EMBO/SourceData huggingface dataset:
Figure legend: "A. Example of electrophysiological fEPSP recording in stratum pyramidale of area CA3 in a control slice (DIV30) during wash-in of 50 µm picrotoxin. This leads to blockage of GABAergic transmission followed by epileptiform discharges (burst) occurring approximately after 3 min. The zoom-in reveals that such an epileptiform discharge (burst) consists of repetitive firings.B. Similar example trace as in (A) recorded in a hTauAT expressing slice (red) demonstrating an increase in burst frequency as well as in firings per burst due to hTauAT expression.C. Quantification of bursts per minute in DIV 10 slices from control littermates and hTauAT slices demonstrating a ~2-fold increase in hTauAT slices (n=8 slices per group).D. Firings per burst are increased ~1.5-fold already at the early time point DIV10 due to hTauAT expression.E. Analysis of burst frequency in slices at DIV 30 demonstrates the progression of the pathological effect beyond that observed at DIV 10. Burst frequency is enhanced in hTauAT expressing slices (n = 10 slices). If Ceftriaxone (CEF) is applied to hTauAT slices the pathological burst frequency enhancement is partly prevented (n = 5 slices).F. Firings per burst are strongly enhanced due to hTauAT expression in slices at DIV 30. Ceftriaxone prevents this pathological phenotype if applied during the entire period of cultivation (n = 6 slices)."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "G. Example of a control littermate organotypic slice culture at DIV 24 stained against NeuN (red) and pan-Tau (K9JA antibody, green). Axonal projections (green) of granule cells (red) in the dentate gyrus are depicted.H. Hippocampal slice culture expressing hTauAT stained against NeuN (red) and pan-Tau (green) showing mossy fiber sprouting in the hilar region, CA3 and dentate gyrus (white arrows)."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "A. Quantification of glutamate concentrations in the medium of control and hTauAT cultures in the presence of either 50 nM tetanus neurotoxin (TeNT) or 1 µM tetrodotoxin (TTX) at DIV5. Under both conditions, glutamate levels in the culture medium of hTauAT slices decreased to control levels (n=6 culture inserts containing 6 slices each / group and condition).B. Quantification of extracellular glutamate concentrations in culture medium of either control or hTauAT slices at DIV 20 in the presence or absence of 50 µM benzyloxy-aspartic acid (TBOA) from DIV1-DIV20 demonstrating a possible contribution of astrocytic glutamate dysregulation to increased extracellular glutamate in hTauAT slices (n=8-9 culture inserts containing 6 slices each / group and condition). One-Way ANOVA F(3,30)=14,89; p&lt;0.001"
NCBI gene IDs:
"Tau: 4137"
Figure legend: "C. Quantification of LDH release into the culture medium of either control or hTauAT slices at DIV 20 in the presence or absence of 50 µM benzyloxy-aspartic acid (TBOA) from DIV 1 to DIV 20 (n=6-12 culture inserts containing 6 slices each / group and condition). One-Way ANOVA followed by Tukey's post-hoc test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "D. Quantification of extracellular glutamate concentrations in either control or hTauAT cultures treated (n=7-8 culture inserts containing 6 slices each / group and condition) with ceftriaxone from DIV 1 to DIV 25 demonstrating the preventative effect of CEF on the increase in extracellular glutamate in hTauAT slices. One-Way ANOVA F(3,25)=9.075; p=0.0003)."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "E. LDH release at DIV 25 is strongly reduced due to long term treatment with ceftriaxone in hTauAT slice cultures (n=8-10 culture inserts containing 6 slices each / group and condition). One Way ANOVA F(3,32)=7.685; p=0.0005)."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "F. Intracellular calcium rise after KCl stimulation is not distinguishable from control slices in hTauAT slices treated with ceftriaxone for 15 days (n=5-9 slices; prepared from at least three animals). One way ANOVA F((3,25)=7.317; p=0.0011)."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "Confirmation of RNA-seq data via qPCR on pure hepatocytes isolated via flow cytometry-based sorting (n = 3/group). (E) Ppara and (F) Hmgcs2 mRNA expression is shown as relative expression, normalized to housekeeping genes Hprt and Rpl. P-values were calculated using 2-way ANOVA analysis."
NCBI gene IDs:
"Hprt: 
Rpl: 
Hmgcs2: 15360
Ppara: 19013"
Figure legend: "Mice (n = 4/group, data are representative of 2 experiments) underwent a sham (with or without starvation) or CLP operation and liver was isolated on several timepoints post-surgery for RNA preparation and qPCR. Ppara mRNA expression is shown as relative expression, normalized to housekeeping genes Hprt and Rpl. P-values were calculated using 1-way ANOVA analysis."
NCBI gene IDs:
"Hprt: 
Rpl: 
Ppara: 19013"
Figure legend: "Pearson correlation between log fold change (LFC) Ppara expression levels and body temperature 24h post-sepsis (n= 38, r = 0.6875, combined data of 4 independent experiments)."
NCBI gene IDs:
"Ppara: 19013"
Figure legend: "Liver Hmgcs2 mRNA expression at different timepoints post-sepsis, expression is shown as relative expression, normalized to housekeeping genes Hprt and Rpl. P-values were calculated using 1-way ANOVA analysis (n = 4/group, data are representative of 2 experiments)."
NCBI gene IDs:
"Hprt: 
Rpl: 
Hmgcs2: 15360"
Figure legend: "Mice were pre-treated with Pemafibrate (1mg/kg) or vehicle (0,9% NaCl) for 1 week before being subjected to CLP. Liver samples were isolated 24h after CLP (n= 5-7/group, combined data of 2 independent experiments), mRNA was prepared and gene expression levels of Ppara was analyzed via qPCR. Gene expression values are shown as relative expression, normalized to housekeeping genes Hprt and Rpl. P-values were calculated via 2-way ANOVA test."
NCBI gene IDs:
"Hprt: 
Rpl: 
Ppara: 19013"
Figure legend: "Dynamic fluorescence patterns according to cell density in 8×GTIIC-EmGFP 293T cells."
NCBI gene IDs:
"EmGFP: "
Figure legend: "EmGFP intensity in LATS2 knockdown, or forced expression of a constitutively active form of YAP (YAP-S127A). Scale bars indicate 100 μm."
NCBI gene IDs:
"LATS2: 26524
YAP: 10413"
Figure legend: "Scatter plots of mean frequency of each shRNA per gene. Average of v HOXA4 is indicated as a red dot."
NCBI gene IDs:
"HOXA4: 3201"
Figure legend: "Augmented 8×GTIIC-luciferase reporter activity by loss of HOXA4. ShSAV1 was used as an experimental control. **P&lt;0.01 vs shCtrl, by ANOVA with Sidak's correction."
NCBI gene IDs:
"HOXA4: 3201
SAV1: 60485"
Figure legend: "Quantitative real-time PCR analysis of ANKRD1 expression in HEK 293T cells transfected with shHOXA4"
NCBI gene IDs:
"ANKRD1: 27063
HOXA4: 3201"
Figure legend: "Quantitative real-time PCR analysis of ANKRD1 expression in HEK 293T cells transfected with HOXA4 expression vector"
NCBI gene IDs:
"ANKRD1: 27063
HOXA4: 3201"
Figure legend: "Quantitative real-time PCR analysis of CTGF, CYR61, and BIRC5 in HEK 293T cells with knockdown of endogenous HOXA4. ShLATS1/2 and shYAP were used as an experimental control. *P&lt;0.05, **P&lt;0.01 vs shCtrl, by ANOVA with Sidak's correction."
NCBI gene IDs:
"BIRC5: 332
CYR61: 3491
CTGF: 1490
HOXA4: 3201
LATS1: 9113
YAP: 10413"
Figure legend: "8×GTIIC-luciferase reporter assays in HEK 293T cells transfected HOXA4 and/or shLATS1/2. **P&lt;0.01 vs shCtrl, by two-way ANOVA with Sidak's correction."
NCBI gene IDs:
"HOXA4: 3201
LATS1: 9113"
Figure legend: "Quantitative real-time PCR analysis of HOXA4, YAP, CTGF, and CYR61 in HEK 293T cells with overexpression of HOXA4 and/or constitutive active form of YAP (YAP -5SA). *P&lt;0.05, **P&lt;0.01 vs YAP-5SA with LacZ, by ANOVA with Sidak's correction."
NCBI gene IDs:
"LacZ: 
CYR61: 3491
CTGF: 1490
HOXA4: 3201
YAP: 10413"
Figure legend: "Quantitative real-time PCR analysis of HOXA4, YAP, CTGF, and CYR61 in HEK 293T cells with knockdown of endogenous HOXA4 and/or overexpression of YAP-5SA. *P&lt;0.05, **P&lt;0.01 vs YAP-5SA with shCtrl, by ANOVA with Sidak's correction."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490
HOXA4: 3201
YAP: 10413"
Figure legend: "Association of HOXA4 with TEAD1 indicated by co-IP experiments. HEK 293T cells were co-transfected with FLAG-HOXA4 and Myc-TEAD1"
NCBI gene IDs:
"FLAG: 
Myc: 
HOXA4: 3201
TEAD1: 7003"
Figure legend: "Association of HOXA4 but not YAP indicated by co-IP experiments. HEK 293T cells were co-transfected with FLAG-HOXA4 and Myc-YAP."
NCBI gene IDs:
"FLAG: 
HOXA4: 3201
Myc: 17869
YAP: 10413"
Figure legend: "Immunoprecipitation using FLAG antibody in lysates from HEK 293T cells transfected with plasmids expressing HOXA4 truncation mutants and TEAD1."
NCBI gene IDs:
"HOXA4: 3201
TEAD1: 7003"
Figure legend: "Immunoprecipitation using FLAG antibody in lysates from HEK 293T cells transfected with plasmids expressing TEAD1 truncation mutants and HOXA4. Arrowheads indicate IgG heavy chain. Arrows indicate TEAD1 (a.a. 1-426)."
NCBI gene IDs:
"HOXA4: 3201
TEAD1: 7003"
Figure legend: "ChIP assays of CTGF and CYR61 promoters using TEAD4 or YAP antibodies in HEK 293T cells transfected with plasmids expressing HOXA4. *P&lt;0.05, **P&lt;0.01, by unpaired two-tailed Student's t-test. Data are expressed as the means ± SEM of three independent experiments."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490
HOXA4: 3201"
Figure legend: "Competitive co-IP assay and densitometry showing significantly reduced TEAD-YAP interaction by overexpression of HOXA4 in a dose-dependent manner in HEK 293T cells. *P&lt;0.05, **P&lt;0.01, by ANOVA with Sidak's correction. Data are presented as mean ± SEM of three independent experiments."
NCBI gene IDs:
"HOXA4: 3201"
Figure legend: "Expression of Hoxa4 in various mouse tissues using qRT-PCR. Expression of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) mRNA was used as an internal control. WAT; white adipose tissue, BAT; brown adipose tissue, Skm; skeletal muscle."
NCBI gene IDs:
"Gapdh: 
glyceraldehyde-3-phosphate dehydrogenase: 
Hoxa4: 15401"
Figure legend: "Quantitative real-time PCR analysis of smooth muscle specific genes in primary human VSMCs transduced with lentiviral vectors encoding overexpression or knockdown of HOXA4 and YAP. Cells infected with lentiviral vectors encoding LacZ or shCtrl were used as controls. *P&lt;0.05, **P&lt;0.01, by unpaired two-tailed Student's t-test."
NCBI gene IDs:
"LacZ: 
HOXA4: 3201
YAP: 10413"
Figure legend: "Representative western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding knockdown of YAP/TAZ Representative western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding knockdown of HOXA4."
NCBI gene IDs:
"HOXA4: 3201
TAZ: 6901
YAP: 10413"
Figure legend: "Representative western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding knockdown of HOXA4 Representative western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding knockdown of HOXA4."
NCBI gene IDs:
"HOXA4: 3201"
Figure legend: "Quantitative real-time PCR analysis of YAP/TEAD target genes in primary human VSMCs transduced with lentiviral vectors encoding overexpression or knockdown of HOXA4 and YAP. The cells infected with lentiviral vectors encoding LacZ or shCtrl were used as controls. *P&lt;0.05, **P&lt;0.01, by unpaired two-tailed Student's t-test."
NCBI gene IDs:
"LacZ: 
HOXA4: 3201
TEAD: 21676
YAP: 10413"
Figure legend: "Proliferation assay in VSMCs transduced with the indicated genes. Virus-transfected human VSMCs were plated at equal density in growth medium, and then cells were collected and counted at each time point as indicated. **P&lt;0.01 vs LacZ, by ANOVA with Sidak's correction."
NCBI gene IDs:
"LacZ: "
Figure legend: "BrdU incorporation assays in VSMCs transduced with the indicated genes. The percentage of BrdU positive cells in each group is shown. Nine to twelve images were randomly acquired and analyzed. **P&lt;0.01 vs LacZ, by ANOVA with Sidak's correction."
NCBI gene IDs:
"LacZ: "
Figure legend: "Representative western blotting analysis of total protein lysates of primary human VSMCs transduced with lentiviral vectors encoding shRNAs of HOXA4 and TEAD1/3/4. Quantification of alpha smooth muscle actin and calponin protein levels is expressed as the means ± SEM of three independent experiments. *P&lt;0.05, **P&lt;0.01, by ANOVA with Sidak's correction."
NCBI gene IDs:
"HOXA4: 3201
TEAD1: 7003"
Figure legend: "Representative immunoblotting images and quantification of the interaction between endogenous TEAD1 and HOXA4 or YAP using co-IP experiments in human VSMCs with knockdown of HOXA4. *P&lt;0.05 vs shCtrl, by ANOVA with Sidak's correction. Data are presented as mean ± SEM of three independent experiments."
NCBI gene IDs:
"HOXA4: 3201"
Figure legend: "ChIP assays of CTGF, CYR61 and α-SMA promoters using anti-FLAG-tag, anti-Myc-tag, anti-TEAD1 antibodies in human VSMCs transfected with plasmids expressing Myc-tagged YAP-5SA and/or FLAG-tagged HOXA4. *P&lt;0.05, **P&lt;0.01 vs FLAG-GFP, by unpaired two-tailed Student's t-test. Data are expressed as the means ± SEM of three independent experiments."
NCBI gene IDs:
"FLAG: 
GFP: 
Myc: 
α-SMA: 59
CYR61: 3491
CTGF: 1490
HOXA4: 3201
YAP: 10413"
Figure legend: "Quantitative real-time PCR analysis of differentiated smooth muscle marker genes in the left carotid artery of 8-week-old WT and Hoxa4 KO mice (WT: n=7, Hoxa4 KO: n=5)."
NCBI gene IDs:
"Hoxa4: 15401"
Figure legend: "Quantitative real-time PCR analysis of differentiated smooth muscle marker genes in the ligated left carotid artery of WT and Hoxa4 KO mice at 1 week after ligation (n=6). Mean expression level of six WT mice at the baseline was defined as 1.0."
NCBI gene IDs:
"Hoxa4: 15401"
Figure legend: "Hoxa4 expression levels in the ligated left carotid artery of WT mice at 1 week after ligation (n=6)."
NCBI gene IDs:
"Hoxa4: 15401"
Figure legend: "Quantitative real-time PCR analysis of Yap/Tead target genes in the left carotid artery of 8-week-old WT and Hoxa4 KO mice (WT: n=7, Hoxa4 KO: n=5)."
NCBI gene IDs:
"Hoxa4: 15401
Tead: 21676
Yap: 22601"
Figure legend: "Quantitative real-time PCR analysis of Yap/Tead target genes in the ligated left carotid artery of WT and Hoxa4 KO mice at 1 week after ligation (n=6). Mean expression level of six WT mice at the baseline was defined as 1.0."
NCBI gene IDs:
"Hoxa4: 15401
Tead: 21676
Yap: 22601"
Figure legend: "Representative western blotting analysis and densitometry of non-ligated (right; R) and ligated (left; L) carotid arteries from WT and Hoxa4 KO mice at 1 week after ligation (WT: n=6, Hoxa4 KO: n=7). *P&lt;0.05, **P&lt;0.01, by two-way ANOVA with Sidak's correction."
NCBI gene IDs:
"Hoxa4: 15401"
Figure legend: "Representative images showing Elastica van Gieson staining (G), and vessel, neointima and media area (H), and neointima to media layer ratio (I) of carotid arteries of WT and Hoxa4 KO mice at 4 weeks after ligation (WT: n=14, Hoxa4 KO: n=11)."
NCBI gene IDs:
"Hoxa4: 15401"
Figure legend: "(B) RNAs were extracted from the cell culture supernatant, and detected using a commercial kit targeting the ORF 1ab (red) and N (blue) genes of SARS-CoV-2."
NCBI gene IDs:
"ORF 1ab: 
N: 43740575"
Figure legend: "B Representative images of transduced adult-born neurons at 17dpi. CAG-dnLEF-IRES-GFP (dnLEF, green) and CAG-RFP (control, red) double transduced cells (arrows) and CAG-RFP single transduced cells (arrowheads). Scale bar= 20 µm"
NCBI gene IDs:
"GFP: 
IRES: 
LEF: 16842"
Figure legend: "C Representative reconstructions of control and dnLEF neurons. Scale bar= 20 µm"
NCBI gene IDs:
"LEF: 16842"
Figure legend: "D Analysis of morphology showed a reduction in dendritic length in dnLEF transduced neurons [p&lt;0.0001], while the number of branch points remained comparable [p=0.7914]. Sholl analysis displayed a reduction in dendritic complexity in dnLEF transduced neurons and indicated decreased growth of terminal dendritic branches [p&lt;0.0001] (control: n= 20 cells from 3 animals, dnLEF: n= 38 cells from 6 animals)."
NCBI gene IDs:
"LEF: 16842"
Figure legend: "E DnLEF neurons displayed basal dendrites [p&lt;0.0001] (control: n= 20 cells from 3 animals, dnLEF: n= 38 cells from 6 animals)."
NCBI gene IDs:
"LEF: 16842"
Figure legend: "G Representative images depicting CAG-GFP-IRES-Cre transduced adult-born neurons in control and β-catex3 mice at 17dpi. Scale bar= 20µm"
NCBI gene IDs:
"Cre: 
GFP: 
IRES: 
β-cat: 12387"
Figure legend: "H Representative reconstructions of control and β-catex3 neurons. Scale bar= 20µm"
NCBI gene IDs:
"β-cat: 12387"
Figure legend: "I Quantification showed decreased dendritic length of β-catex3 neurons [p&lt;0.0001]; no difference was apparent in number of branch points [p=0.3256]. Sholl analysis displayed a less complex dendritic tree of β-catex3 neurons [p&lt;0.0001] (control: n= 20 cells from 5 animals, β-catex3: n= 20 cells from 5 animals)."
NCBI gene IDs:
"β-cat: 12387"
Figure legend: "J The number of basal dendrites was increased in β-catex3 neurons [p=0.0003] (control: n= 20 cells from 5 animals, β-catex3: n= 20 cells from 5 animals)."
NCBI gene IDs:
"β-cat: 12387"
Figure legend: "B Representative reconstructions of control and β-catex3 iDCX neurons at 3dpT. Scale bar= 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "C Analysis of morphology showed increased dendritic length in β-catex3 iDCX neurons at 3dpT [p=0.0320]. The number of branch points was unaltered [p=0.0651]. Sholl analysis indicated increased growth of terminal dendrite branches in neurons of β-catex3 iDCX mice [p&lt;0.0001] (control: n = 25 cells from 9 animals; β-catex3 iDCX: n = 25 cells from 8 animals). D Analysis of morphology showed no difference in dendritic length [p=0.1029] and branch point number [p=0.3726] between experimental groups at 13dpT. Sholl analysis indicated a decrease in length of terminal dendrites in neurons of β-catex3 iDCX mice [p&lt;0.01] (control: n= 23 cells from 9 animals, β-catex3 iDCX: n= 26 cells from 8 animals). "
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "E Spine densities were comparable between experimental groups at 3dpT [iML p=0.1512, oML p=0.3841]. 13dpT β-catex3 iDCX neurons showed significantly increased spine density in the inner molecular layer [iML p&lt;0.0001 , oML p=0.0402] (3dpT: control: n= 20 cells from 3 animals, β-catex3 iDCX: n= 20 cells from 4 animals; 13dpT control: n= 20 cells from 5 animals, β-catex3 iDCX: n= 20 cells from 7 animals). Representative images showing dendritic segments in the inner molecular layer of the DG at 13dpT. Scale bars= 1µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "F Representative images of recombined (GFP+) neurons co-expressing Prox1 (grey) as a marker for neuronal fate and the stage specific markers DCX (red) for immature neurons and Calbindin (red) for mature neurons. Arrows and arrowheads indicate marker negative and marker positive cells, respectively. Scale bar = 10µm. G Fraction of DCX expressing neurons is slightly reduced in β-catex3 iDCX at 3dpT [p=0.0338], while fraction of Calbindin expressing cells is slightly increased in β-catex3 iDCX at 3dpT [p=0.0345]. At 13dpT no difference is visible in stage specific marker expression [DCX p=0.8564, Calbindin p=0.0841] (3dpT: control: n= 12 animals, β-catex3 iDCX: n= 12 animals, 13dpT: control: n= 10 animals, β-catex3 iDCX: n= 14 animals). "
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "B Expression of DCX and Calbindin in BrdU+ neurons was comparable between experimental groups at 3dpT and 13dpT (control: n= 5 animals, β-catex3 iDCX: n= 5 animals)."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "C Representative reconstructions of control and β-catex3 iDCX neurons expressing RFP at 3dpT. Scale bar= 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "D Quantification of dendritic length [p=0.0003], branch points [p=0.0433], and Sholl analysis [p&lt;0.0001] showed a higher dendritic complexity of β-catex3 iDCX neurons at 3dpT (control: n= 22 cells from 4 animals, β-catex3 iDCX: n= 19 cells from 5 animals)."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "E Representative reconstructions of control and β-catex3 iDCX neurons expressing RFP at 13dpT. Scale bar= 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "F Quantification of dendritic length [p&lt;0.0001], and branch points [p=0.0495], and Sholl analysis [p&lt;0.0001] showed a lower dendritic complexity of β-catex3 iDCX neurons at 13dpT (control: n= 20 cells from 4 animals, β-catex3 iDCX: n= 19 cells from 5 animals)."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "B Representative reconstructions of control and β-catex3 iDCX neurons in 24-week old mice at 3dpT. Scale bar = 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "C Dendritic length [p&lt;0.0001] and number of branch points [p&lt;0.0001] was increased in 24-week-old β-catex3 iDCX compared to 24-week-old controls at 3dpT (control: n= 21 cells from 5 animals, β-catex3 iDCX: n= 20 cells from 6 animals). At 13dpT dendritic length [p=0.5788] and number of branch points [p=0.4978] were comparable (control: n= 21 cells from 5 animals, β-catex3 iDCX: n= 20 cells from 8 animals)."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "D Representative images showing dendritic segment of the inner molecular layer of the DG in 24-week old mice at 13dpT. Quantification of the number of spines showed increased spine density in inner molecular and outer molecular layer in 24-week old β-catex3 iDCX mice at 13dpT [iML p=0.0047, oML p&lt;0.0001] (control n=19 cells from 4 animals, β-catex3 iDCX: n= 20 cells from 4 animals). Scale bars = 1µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "E Representative images of recombined (GFP+, green) neurons in 24-week old animals at 13dpT co-expressing Prox1 (grey) as a marker for neuronal fate and the stage specific markers DCX (red) for immature neurons and Calbindin (red) for mature neurons. Arrows and arrowheads indicate marker negative and marker positive cells, respectively. Scale bar= 10µm. F Quantification of marker expression. The fraction of Calbindin expressing cells was increased in the recombined population in β-catex3 iDCX at 3dpT [p=0.0081] and 13dpT [p=0.0259] (3dpT: control: n= 4 animals, β-catex3 iDCX: n= 9 animals, 13dpT: control: n= 8 animals, β-catex3 iDCX: n= 10 animals). "
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "B Representative images of recombined (GFP+, green) BrdU+ (grey) neurons in 24-week old control and β-catex3 iDCX animals at 13dpT expressing the stage specific markers DCX (red) for immature neurons and Calbindin (red) for mature neurons. Arrows and arrowheads indicate marker negative and marker positive cells, respectively. Scale bar= 10µm. C Quantification of DCX and Calbindin expression in BrdU+ recombined cells. At 13dpT, labeled neurons in β-catex3 iDCX animals show a shift towards a more mature marker profile [DCX p=0.0238, Calbindin p=0.0714] (3dpT: control: n= 4 animals, β-catex3 iDCX: n= 8 animals; 13dpT control: n= 5 animals, β-catex3 iDCX: n= 5 animals). "
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "D Representative reconstructions of RFP-birthdated control and β-catex3 iDCX neurons in 24-week old mice at 3dpT. Scale bar= 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "E Quantification of dendritic length [p=0.0007], branch points [p=0.3909], and Sholl analysis [p=0.0001] showed increased complexity of birthdated β-catex3 iDCX cells at 3dpT (control: n= 20 cells from 4 animals, β-catex3 iDCX: n= 19 cells from 5 animals)."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "F Representative reconstructions of RFP-birthdated control and β-catex3 iDCX neurons in 24-week old mice at 13dpT. Scale bar= 20µm."
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"
Figure legend: "G Quantification of dendritic length [p=0.1857], branch points [p=0.1331], and Sholl analysis [p=0.1501] showed no difference between β-catex3 iDCX and control cells at 13dpT (control: n= 18 cells from 4 animals, β-catex3 iDCX: n= 20 cells from 4 animals)"
NCBI gene IDs:
"β-cat: 12387
DCX: 13193"