EMBO/SourceData huggingface dataset:
Figure legend: "A generalized seizure is depicted in Fig. EV1 D and E: The characteristic EEG features of this model, secondary generalized seizures (bars) and HPDs (lines) were reduced in number and in duration by AAV-pDyn (red; n = 3 (from day 60); 7 (till day 30) per time-interval), but not by AAV-ΔGFP or after sham treatment (blue; n = 4 (day 90); 5 (day 30 and 60); 6 (before day 30), per time-interval). The relatively high variability of seizure frequencies and duration is typical for this model and reflects the findings in human mTLE."
NCBI gene IDs:
"GFP: 
pDyn: 5173"
Figure legend: "Injection of norBNI (20 mg/kg; i.p.) results in a transient reappearance of HPDs immediately and 24 hrs after application. One week after norBNI application (washout) suppression of HPDs was re-established. Data obtained from 4 epileptic animals before (black) and after (red) AAV-pDyn delivery (2x109 gp) are depicted. *"
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "EEG recordings obtained from the ipsilateral dorsal hippocampus of rats after electrical self-sustained status epilepticus (SSSE) before (black) and after (red) AAV-pDyn delivery (4x109 gp) are depicted. Spike trains with a frequency of at least 2.5 Hz induced by SSSE were markedly reduced by AAV-pDyn (n=4)."
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "Spatial learning and memory was tested on the Barnes maze. Quadrant 1 (Q1) contains the target hole (red; A). Unilateral injection of AAV-pDyn (B) or AAV-eGFP (C) into naïve young adult mice (12 weeks age) did not influence the performance as compared to naïve controls (D) when tested 4 weeks after AAV injection. Mice treated two weeks after KA with AAV-pDyn (E,H) performed equally to age-matched naïve controls (D,G) 2 weeks (E) and 5.5 months (H) after treatment. By contrast, animals treated two weeks after KA with AAV-ΔGFP (F,I) gradually lost this ability. Two-way ANOVA revealed significance between AAV-ΔGFP and AAV-pDyn treated groups for interaction 2 weeks (p = 0.0349) and 5.5 months (p = 0.0311) after AAV, respectively, at each time interval and quadrant (p &lt; 0.0001) 2 weeks after AAV. Comparison of AAV-pDyn injected with naïve animals revealed no differences."
NCBI gene IDs:
"eGFP: 
GFP: 
pDyn: 5173"
Figure legend: "epileptic mice, which were not able to learn the Barnes maze task one month after KA (J, M), AAV-pDyn application restored spatial memory one (K) and 2 months (L) after treatment."
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "epileptic mice, which were not able to learn the Barnes maze task one month after Treatment with AAV-ΔGFP did not result in improved memory (N,O)."
NCBI gene IDs:
"GFP: "
Figure legend: "Double-immunofluorescence labeling is depicted for pDyn and NeuN (A-C) or GFAP (D-F) in the ipsilateral dentate gyrus of KA-treated and AAV-pDyn-injected mice. Enlarged view in (F) represents 15 x 30 µm."
NCBI gene IDs:
"pDyn: 5173"
Figure legend: ": mature Dyn B content (measured by a Dyn B specific EIA) in the dorsal hippocampus of mice treated with KA (blue symbols) or KA and AAV-pDyn (red symbols) 1.5 (open symbols; n = 6) and 6 (filled symbols; n = 3) months after vector treatment. Naïve animals were age matched to the 1.5 months after AAV group. iH stands for ipsilateral hippocampus and cH for contralateral hippocampus.* = p&lt;0.05; ** = p&lt;0.01; paired t-test was used for comparison of ipsi- and contralateral hippocampi. Two-way ANOVA was used to compare Dyn levels between the early and late time interval."
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "Mature Dyn B content in the CSF of mice treated with KA (blue symbols) or KA and AAV-pDyn (red symbols) 1.5 (open symbols; n= 6) and 7 (filled symbols; n = 4) months after vector treatment. ** = p&lt;0.01; one-way ANOVA with Dunnett post hoc test"
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "In-situ hybridization for AAV-derived pDyn mRNA at 10 weeks post AAV-pDyn delivery in the left dorsal hippocampus is depicted for a single brain at five levels between 1.1 (left) and 2.3 (right) mm from bregma. The probe specifically detects the codon-optimized sequence, therefore the contralateral side represents a control devoid of the respective mRNA."
NCBI gene IDs:
"pDyn: 5173"
Figure legend: "At 10 weeks post AAV-pDyn delivery, markers of inflammation were analyzed as follows: Relative optical densities (ROD) were evaluated from film autoradiographs after in-situ hybridization for mRNAs of inflammatory markers (Interleukin-1β (B), Interleukin-6 (C), Interleukin1a receptor agonist (D), Tumor Necrosis Factor-α (E) and Nitric Oxide Synthase-1 (F)) in 3 principal sub-regions of the hippocampus in AAV-pDyn (n=7; red) or AAV-eGFP (n=5; blue) injected animals. GC = granule cell layer; CA = cornu ammonis."
NCBI gene IDs:
"eGFP: 
Interleukin-1β: 16176
Interleukin1a receptor agonist: 16181
Interleukin-6: 16193
Nitric Oxide Synthase-1: 18125
pDyn: 5173
Tumor Necrosis Factor-α: 21926"
Figure legend: "Male C57BL/6J mice were fed with chow diet supplemented with 0, 0.5%, 1% SUC for 8 weeks. mRNA expression of MyHC I, MyHC IIa, PGC-1α, myoglobin, TnnT1 MyHC IIb, MyHC IIx and TnnT3 in the gastrocnemius muscle (n=5-6)."
NCBI gene IDs:
"myoglobin: 17189
MyHC I: 17879
MyHC IIx: 17879
MyHC IIa: 17882
MyHC IIb: 17884
PGC-1α: 19017
TnnT1: 21955
TnnT3: 21957"
Figure legend: "The mRNA level of SUNCR1 in gastrocnemius and soleus (n=7-8)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "SUNCR1 protein expression in C2C12 cells transfected with vector or siSUNCR1 (n=3)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "[Ca2+]i, in vector or siSUNCR1 transfected C2C12 cells treated with 0 or 2 mM SUC (n=5-6)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "enzymes activity (n=9-10)of (J) HK, (K) LDH, and (L) SDH in vector or siSUNCR1 transfected C2C12 cells treated with 0 or 2 mM SUC (n=5-6)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. The O2 consumption (VO2), RER"
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. muscle grip strength"
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. four-limb handing time"
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. low speed running time."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. The enzymes activity of (H) HK, (I) LDH, and (J) SDH in gastrocnemius."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. Immunoblots and quantification of MyHC-I, IIb, NFAT, and PGC-1α protein in gastrocnemius."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J or SUNCR1 KO mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. Representative images and quantification of Laminin (green), or MyHC-I and IIb (red) immunofluorescent staining in gastrocnemius muscle (n=3). Scale bar in (M) represents 100 µm."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. SUNCR1 protein expression in gastrocnemius from mice transfected with shSUNCR1 lentivirus or LV-shScrambled (n=3)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. The running time in low speed"
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. four-limb handing time"
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. muscle grip strength of both control and gastrocnemius-specific SUNCR1 knockdown mice."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. The enzymes activity of (F) HK, (G) LDH, (H) SDH in gastrocnemius."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "Male C57BL/6J mice were injected with LV-shScrambled or shSUNCR1 lentivirus specifically into the gastrocnemius at 6 weeks of age. After two weeks of recovery, mice were fed with chow diet supplemented with 0 or 1% SUC for 6 weeks. Immunoblots and quantification of MyHC-I, IIb, NFAT, and PGC-1α protein in gastrocnemius (n=3)."
NCBI gene IDs:
"SUNCR1: 84112"
Figure legend: "E Effect of H2A.Z.2.2 ROF on facilitating histone replacement. Incorporations of yeast H2A.Z-H2B dimers containing either the wild type H2A.Z or Z.2.2-like H2A.Z were analyzed by SWR1-catalyzed histone replacement assay. H2A.Z and Z.2.2-like H2A.Z incorporated into nucleosome were resolved by 6% Native-PAGE and detected by SYBR Green staining and western blotting."
NCBI gene IDs:
"H2B: 852284///851810"
Figure legend: "b, TG levels in vector-infected (VEC) and siAtg5 cells in RM, OL or in MCDM (*P  0.001, n = 5). OL values are in mM."
NCBI gene IDs:
"Atg5: 365601"
Figure legend: "c, TG levels in wild-type (WT) or Atg5 knockout mice embryonic fibroblasts (Atg5-/-) (*P 0.01 or **P 0.0001, n = 5)."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "a, b, VEC and siAtg5 cells cultured with oleate (OL) or in MCDM were examined for their rates of TG synthesis (a) and β-oxidation (b) as compared to cells in regular medium alone (*P  0.03, **P  0.004 with VEC cells in the same medium, n = 34)."
NCBI gene IDs:
"Atg5: 365601"
Figure legend: "D Cardiac mRNA expression levels normalized to HPRT. One-way ANOVA; n = 5 mice per ZT; ZT5 vs. ZT17: *P = 0.0064 (CXCL1), *P = 0.0007 (CXCL2), *P = 0.0007 (CXCL5), *P = 0.0001 (ICAM-1), *P = 0.0009 (VCAM-1), *P = 0.0181 (CCL3), *P = 0.0360 (CCL5)."
NCBI gene IDs:
"HPRT: 
CCL3: 20302
CCL5: 20304
CXCL1: 14825
CXCL2: 20310
CXCL5: 20311
ICAM-1: 15894
VCAM-1: 22329"
Figure legend: "F Mean fluorescence intensity (MFI) of CXCX2 expression at ZT5 in the blood 24h after MI in wildtype (WT) and CXCR2 KO mice. Student`s t-test. N = 6 mice in both groups; *P = 0.0001."
NCBI gene IDs:
"CXCR2: 12765"
Figure legend: "G Percentage of CXCR2hi bloodneutrophils 24h after ZT5 or ZT13 MI in WT and CXCR2 KO mice. Two-way ANOVA followed by Bonferroni post-hoc test; for ZT5 n = 6 mice in both groups, for ZT13 n = 7 WT mice and n = 5 CXCR2 KO mice; WT vs. CXCR2 KO: *P = 0.0001 (ZT13)."
NCBI gene IDs:
"CXCR2: 12765"
Figure legend: "H Flow cytometry quantification of neutrophils in hearts 24h after MI in ZT5 and ZT13 operated WT and CXCR2 KO mice. Two-way ANOVA followed by Bonferroni post-hoc test; for ZT5 n= n = 6 mice in both groups, for ZT13 n = 7 WT mice and n = 5 CXCR2 KO mice; WT vs. CXCR2 KO: *P = 0.0461 (ZT13), ns = not significant."
NCBI gene IDs:
"CXCR2: 12765"
Figure legend: "a, Electron microscopy analysis of wild-type (WT) and Smurf1-/- (KO) MEFs grown in normal media or EBSS (starvation) for 4 h. Arrowheads, representative autophagosomes; arrows, representative autolysosomes. Scale bars, 500 nm."
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "a, Representative mitochondrial morphology in Smurf1+/+ (wild-type) and Smurf1−/− (KO) MEFs transfected with indicated construct and treated with DMSO or 10 µM CCCP for 24 h. b, Quantification of percentage of total cells with a diffuse accumulation of abnormal fragmented mitochondria and lack of mitochondrial clearance. Results shown represent combined data from 3-5 experiments per condition with triplicate wells (of at least 100 cells per well) analysed for each condition per experiment. Shown are mean ± s.e.m. for average values from each experiment. Similar results were observed in each independent experiment. *P  0.001, Students t-test. c, Measurement of mitochondrial fractional area (percentage of total cellular area) in MEFs treated as in a. Results shown represent mean ± s.e.m. for 50 cells per condition."
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "d, Representative confocal micrographs of KO MEFs transfected with YFP-SMURF1 wild-type or YFP-SMURF1Dgr;C2 (Dgr;C2) and treated for 4 h with DMSO or CCCP."
NCBI gene IDs:
"SMURF1: 75788"
Figure legend: "e, Representative confocal micrographs of KO MEFs transfected with GFP-LC3 and wild-type mCherry-SMURF1 (WT) or mCherry-SMURF1Dgr;C2 (Dgr;C2) and treated for 4 h with CCCP. Inset, upper right, formation of completed autophagosome around a damaged mitochondrion associated with wild-type SMURF1; insets, lower right, incomplete autophagosomes or absence of LC3 signal around mitochondria associated with SMURF1Dgr;C2. See also Supplementary Figs 10 and 11 for enlarged images."
NCBI gene IDs:
"LC3: 66734
SMURF1: 75788
SMURF1: Q9CUN6///75788"
Figure legend: "f, Representative electron microscopy images of indicated tissues from 10-month-oldSmurf1+/+ (WT) or Smurf1-/- (KO) mice. Arrows, representative abnormal mitochondria. Scale bars, 1 µm. Similar abnormalities were observed throughout entire electron microscopy tissue section for each mouse and in tissue samples from three different mice for each genotype."
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "A Germination of dog1-3 (knock-out), WT, and dog1-5 (DOG1 upregulation) seeds in media supplemented with NaCl for the indicated number of days after stratification (DAS). Scale bar represents 5 mm. B Germination rate in 100 mM NaCl 7 days after stratification for seeds of different genotypes. Lines represent the fitted curves with a 95% confidence interval (grey area). ∗ p-value &lt; 0.05 from two-tailed Student's t-test. Data for WT is the same as plotted in Fig 3C. C"
NCBI gene IDs:
"DOG1: 834623
dog1: 834623"
Figure legend: "C shDOG1 expression levels normalized to UBC21 (AT5G25760) in dog1-3 and dog1-5 relative to WT. D shDOG1 and lgDOG1 expression levels normalized to UBC21 in seeds treated with 100 mM NaCl relative to mock. E"
NCBI gene IDs:
"DOG1: 834623
dog1: 834623
UBC21: 832645"
Figure legend: "A 3'RNA-seq read coverage on DOG1 locus. Black colour is used for coverage within the scale on the right-hand side (0 to 500), and red is used for coverage within the scale on the left-hand side (0 to 50). Above is a schematic representation of the annotated transcripts from the DOG1 locus and the chromosome coordinates."
NCBI gene IDs:
"DOG1: 834623"
Figure legend: "Germination time-course in 100 mM NaCl after stratification for puppies-1 (C) relative to WT. Lines represent fitted curves with a 95% confidence interval (grey area), dots represent data points, ∗ p-value &lt; 0.05 from the two-tailed Student's t-test. (C) Data for WT is the same as plotted"
NCBI gene IDs:
"puppies-1: 823785"
Figure legend: "F RT-qPCR fold-change induction of PUPPIES-uns, PUPPIES-prom, PUPPIES-fusion, shDOG1, and lgDOG1 in puppies-ox relative to WT (blue dashed line), in dry (light grey) and imbibed seeds in the presence of 100 mM NaCl (red). Significance from the two-tailed Student's t-test for comparing dry seeds of WT and puppies-ox is represented with light grey asterisks, and salt-imbibed seeds of WT and puppies-ox are represented with red asterisks. Black asterisks represent significance from two-way ANOVA with Tukey's multiple comparisons test for dry seeds vs imbibed seeds. ns p-value &gt; 0.05, ∗ p-value &lt; 0.05, ∗∗ p-value &lt; 0.01, ∗∗∗ p-value &lt; 0.001. Error bars represent the mean ± SD. n = 4 biological replicates."
NCBI gene IDs:
"PUPPIES: 
puppies: 
DOG1: 834623"
Figure legend: "G RT-qPCR with primers for shDOG1 and lgDOG1 on nascent RNA from seeds imbibed in 100 mM NaCl from puppies-ox relative to WT. Nascent RNA levels were normalized to UBC21. Bars and error bars represent the mean ± SD. Points represent biological replicates. Statistical significance from two-tailed Student's t-test. ∗ p-value &lt; 0.05."
NCBI gene IDs:
"puppies: 
DOG1: 834623
UBC21: 832645"
Figure legend: "A z-stack max-projection image of smFISH for DOG1 RNA. The \"Inferno\" colour scale is used for the intensity of fluorescence from Quasar670 fluorophore (DOG1). The blue colour shows fluorescence from DAPI (nuclei staining). Arrowheads point to foci corresponding to transcription sites (TS). The scale bar is 20 μm. B Distribution of cytoplasmic DOG1 foci per cell in WT. The blue vertical dashed line indicates the average. C"
NCBI gene IDs:
"DOG1: 834623"
Figure legend: "G z-stack max-projection images from seeds imbibed in 100 mM NaCl of WT (left) versus puppies-ox (right). Arrowheads point to foci corresponding to TS."
NCBI gene IDs:
"puppies: "
Figure legend: "A-E The profile of Pol II during DOG1 transcription. Plots show the fraction of unique reads from targeted NET-Seq using rolling median (11 nt). Targeted libraries for sequencing of DOG1 locus were obtained by usage of 5 primers across the gene. Pol II dynamics is analyzed separately for each region sequenced in exon 1 (A), exon 2 (B), exon 3 (C), intron 1 (D), and intron 2 (E). The x-axis shows the distance in bp from DOG1. A light red colour fill highlights the regions where Pol II density is higher in puppies-ox. A light blue colour fill highlights the regions where Pol II density is higher in WT. For each plot, coloured tiles represent the fold-change (puppies-ox / WT) on a logarithmic scale. Greyscale tiles represent the result of a statistical test for the difference between genotypes from the two-tailed Student's t-test after Bonferroni correction. Tiles correspond to a 25 bp bin each. Vertical dashed lines connect the exon boundaries on the plots with the schematics of the DOG1 gene with grey boxes representing exons and black lines representing introns."
NCBI gene IDs:
"puppies: 
DOG1: 834623"
Figure legend: "F, G RT-qPCR measurement of alternative splicing of lgDOG1 alpha and beta isoforms and unspliced intron 1 DOG1 isoforms levels in puppies-ox, and puppies-1 relative to WT, in seeds treated with 100 mM NaCl."
NCBI gene IDs:
"puppies: 
puppies-1: 823785
DOG1: 834623
DOG1 alpha: 834623
beta: 834623"
Figure legend: "D.)  Live epifluorescence microscopy of the PfMev api-EcDPCK-mCherry Δdpck parasite line that is expressing api-SFG (green) and api-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue). E.) Live epifluorescence microscopy of the  PfMev api-EcDPCK-mCherry Δdpck line after treatment with 1x azithromycin for 7 days under supplementation with 50μM mevalonate. This parasite line expresses  api-SFG (green) and api-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue). Data information: All microscopy images represent fields that are 10µm long by 10µm wide."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
dpck: 811997"
Figure legend: "F.) Attempted  PCR detection of the ldh,  sufB, and cox1 genes from the nuclear (N), apicoplast (A), and mitochondrial (M) genomes, respectively. We failed to amplify sufB from the azithromycin-treated (+ Azith) PfMev api-EcDPCK-mCherry Δdpck parasites, indicating the loss of the  apicoplast  organellar genome."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
ldh: 814112
dpck: 811997
sufB: 812100
cox1: 812199///811957"
Figure legend: "B.) Live epifluorescence microscopy of the  PfMev EcDPCK-mCherry line. This parasite line expresses api-SFG (green) and EcDPCK-mCherry (red) with DAPI marking the nuclei  (blue). D.) Live epifluorescence microscopy of the PfMev EcDPCK-mCherry Δdpck line. This parasite line expresses api-SFG (green) and EcDPCK-mCherry (red) with DAPI marking the nuclei  (blue). Data information: All microscopy images represent fields that are 10µm long by 10µm wide."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
dpck: 811997"
Figure legend: "E.) Attempted  PCR detection of the  ldh, sufB, and cox1 genes from the nuclear (N), apicoplast  (A), and mitochondrial (M) genomes, respectively. We were successful in amplifying  sufB from the PfMev EcDPCK-mCherry Δdpck parasite line grown under continuous supplementation with 50μM mevalonate, indicating retention of the  apicoplast  organellar genome."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
ldh: 814112
dpck: 811997
sufB: 812100
cox1: 812199///811957"
Figure legend: "C.)  Live epifluorescence microscopy of the PfMev CLD-EcDPCK-mCherry-apt Δdpck line. This parasite line expresses api-SFG (green) and CLD-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue). This parasite line was grown in the presence of 0.5μM aTc. D.) The  PfMev CLD-EcDPCK-mCherry-apt Δdpck parasite line grown in the absence of aTc for 48 hours. The brightness of the red fluorescence image was increased to demonstrate the lack of mCherry signal associated with the parasite. E.) The PfMev CLD-EcDPCK-mCherry-apt Δdpck parasite line grown in the presence of 0.5μM aTc, and in the presence of 0.5μM Shield1 (Shld) for 48 hours. F.) The PfMev CLD-EcDPCK-mCherry-apt Δdpck parasite line grown in the absence of aTc, and in the presence of 0.5μM Shield1 (Shld) for 48 hours. The brightness of the red fluorescence image was increased to demonstrate the lack of mCherry signal associated with the parasite. Data information: All microscopy images represent fields that are 10µm long by 10µm wide."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
dpck: 811997"
Figure legend: "G.) Growth curve of the  PfMev CLD-EcDPCK-mCherry-apt Δdpck parasite line grown either in the presence of 0.5μM  aTc  and the absence 0.5μM Shield1 (permissive condition, blue), or the absence of 0.5μM  aTc and the presence of 0.5μM Shield1  (non-permissive condition, red). Pantothenate was supplied at 50nM in both conditions and the cultures were diluted 1:5 on day 4. Treatment under non-permissive conditions resulted in reduced parasite growth beginning at day 1 (two-way ANOVA, followed by Bonferroni's correction; *, P &lt; 0.05). Error bars represent the standard error of the mean from two independent experiments, each conducted in quadruplicate."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
dpck: 811997"
Figure legend: "A.) Attempted  PCR detection of the  ldh, sufB, and cox1 genes from the  nuclear  (N), apicoplast (A), and  mitochondrial  (M) genomes, respectively. We were unsuccessful in amplifying sufB  from the  PfMev CLD-EcDPCK-mCherry-apt Δdpck  parasite line after treatment with 100nM azithromycin  (Azith) in the presence of 50μM mevalonate, indicating the disruption of the  apicoplast organelle."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
ldh: 814112
dpck: 811997
sufB: 812100
cox1: 812199///811957"
Figure legend: "B.) Live epifluorescence microscopy of the apicoplast-negative PfMev CLD-EcDPCK-mCherry-apt Δdpck line post treatment with 100nM azithromycin in the presence of 50μM mevalonate. This parasite line expresses  api-SFG (green) and the CLD-EcDPCK-mCherry construct (red) with DAPI  marking the nuclei  (blue).  Images represent fields that are 10µm long by 10µm wide."
NCBI gene IDs:
"mCherry: 
DPCK: 949060
dpck: 811997"