EMBO/SourceData huggingface dataset:
Figure legend: "representative images showing robust association of Rheb with depolarized mitochondria within axons treated with CCCP. Rheb-SSVM, a farnesylation deficient mutant of Rheb, fails to be recruited to mitochondria upon dissipating ∆ψm. Mitochondria associated with Rheb were marked by white arrows."
NCBI gene IDs:
"Rheb: 6009"
Figure legend: "F-H Representative dual-channel kymographs (F) and quantitative analysis (G, H) showing that Rheb-mediated mitophagy activation depends on Rheb recruitment to mitochondria through Rheb farnesylation. Note that Rheb, but not Rheb-SSVM, is associated with autophagic vacuoles (AVs) within axons upon ∆ψm dissipation. Rheb-associated AVs move in a predominant retrograde direction along axons. The number of Rheb-associated AVs (Rheb-AVs) with or without CCCP treatment and the relative motilities of AVs and Rheb-AVs in CCCP-treated axons were quantified, respectively"
NCBI gene IDs:
"Rheb: 6009"
Figure legend: "Representative dual-channel kymographs showing that Rheb enhances mitophagy in CCCP-treated axons."
NCBI gene IDs:
"Rheb: 6009"
Figure legend: "quantitative analysis showing that Rheb enhances mitophagy in CCCP-treated axons. The numbers of mitochondria (Mito), AVs, and Mito-AVs/mitophagosomes, the percentage of Mito within AVs, were quantified"
NCBI gene IDs:
"Rheb: 6009"
Figure legend: "quantitative analysis the percentage of Mito within AVs, and the motilities of AVs and Mito-AVs along axons with and without elevated Rheb expression were quantified"
NCBI gene IDs:
"Rheb: 6009"
Figure legend: "D,E Representative images (D) and quantitative analysis (E) showing that mitophagy in axons requires Rheb and Nix, but not Parkin. The number of mitophagosomes indicated by white arrows and the percentage of Mito within AVs in CCCP-treated axons expressing scrambled shRNA, Rheb shRNA, Nix shRNA, or Parkin shRNA were quantified, respectively (E)."
NCBI gene IDs:
"Nix: 12177
Parkin: 50873
Rheb: 19744"
Figure legend: "Quantitative analysis showing that Rheb RNAi augments axonal retention of oxidized mitochondria upon ∆ψm depolarization. The fluorescence of MitoROGFP was emitted at 510 nm and excited at 405 nm or 488 nm, respectively. Mean fluorescence intensity ratios evoked by the two excitation wavelengths at individual mitochondria in the CCCP-treated axons expressing scrambled shRNA or Rheb shRNA were quantified and normalized to those of control neurons transfected with scrambled shRNA (H)."
NCBI gene IDs:
"Rheb: 19744"
Figure legend: "representative images showing that Rheb RNAi augments axonal retention of oxidized mitochondria upon ∆ψm depolarization. The fluorescence of MitoROGFP was emitted at 510 nm and excited at 405 nm or 488 nm, respectively. Ratiometric images were generated from fluorescence excited by 405 nm light relative to that excited by 488 nm light. The ratio has been false colored with the indicated heat map, with high intensity indicative of ROGFP fluorescence in a more oxidative level"
NCBI gene IDs:
"Rheb: 19744"
Figure legend: "Aberrant accumulation of mitophagosomes co-labeled by antibodies against LC3 and cytochrome c (Cyto c) in the hippocampal mossy fibers of mutant hAPP Tg mouse brains. Note that mitophagosomes indicated by white arrows are not readily observed in WT mouse brains"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "Quantitative analysis showing presynaptic retention of mitophagosome-like structures in the hippocampal regions of mutant hAPP mouse brains. Data were quantified from the total number of the imaging fields (n) indicated in parentheses"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "representative TEM images showing presynaptic retention of mitophagosome-like structures in the hippocampal regions of mutant hAPP mouse brains. White arrow-labeled mitophagosomes are not readily detected in WT mouse brains. Black arrows marked amphisomes or degradative autophagic vacuoles (AVd)."
NCBI gene IDs:
"hAPP: 351"
Figure legend: "F,G Mitophagic accumulation at Aβ-enriched synapses in AD mouse brains. Note that the levels of mitophagic/autophagic proteins (red box) along with Aβ (green box) are increased in synaptic fractions isolated from AD mouse brains. Equal amounts (10 μg) of synaptosomal preparations (Syn) and post-nuclear supernatants (PNS) from WT and mutant hAPP mouse brains were sequentially immunoblotted with antibodies against hAPP/Aβ, Rheb, LC3, p62, and Rab7, as well as mitochondrial proteins TOM20 and SOD2 on the same membrane after stripping between each antibody application. The purity of synaptosomal fractions was confirmed by relative enrichment of SYP and less abundance of p115 and GAPDH (F). Relative protein levels in the synaptosomal preparations from mutant hAPP mice were compared to those in WT littermate controls. Data were quantified from five repeats (G)."
NCBI gene IDs:
"hAPP: 351"
Figure legend: "Representative images showing increased mitochondrial association with Rheb in AD axons, relative to that of WT littermate controls. Note that CCCP treatment enhances Rheb localization to mitochondria. The percentages of white arrow-marked mitochondria that were targeted by Rheb in WT and mutant hAPP axons in the absence and presence of CCCP"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "quantitative analysis The percentages of white arrow-marked mitochondria that were targeted by Rheb in WT and mutant hAPP axons in the absence and presence of CCCP were quantified, respectively"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "C- Representative dual-channel kymographs showing defective retrograde transport and mitophagic accumulation within AD axons. mitochondria within AVs in WT or mutant hAPP axons with and without CCCP were quantified"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "quantitative analysis , the numbers of Mito, AVs, and Mito-AVs, and the percentage of mitochondria within AVs in WT or mutant hAPP axons with and without CCCP were quantified, respectively"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "quantitative analysis Relative motility of AVs and Mito-AVs/mitophagosomes in WT or mutant hAPP axons with and without CCCP were quantified"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "F,G Quantitative analysis (F) and representative blots (G) showing that ∆ψm depolarization augments mitophagy activation in cortical neurons cultured from AD mouse brains. Note increased localization of Rheb along with LC3-II and Rab7 to mitochondria in mutant hAPP Tg neurons after CCCP treatment (red box). Data were quantified from three independent repeats. P: post-nuclear supernatants; M: mitochondria-enriched membrane fractions; S: cytosolic supernatants."
NCBI gene IDs:
"hAPP: 351"
Figure legend: "Quantitative analysis Mean fluorescence intensity ratios evoked by the two excitation wavelengths (405 nm and 488 nm) at individual mitochondria in mutant hAPP axons with and without CCCP treatment were quantified and normalized to those of WT controls"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "representative images showing abnormal accumulation of oxidatively damaged mitochondria in AD axons, which is exacerbated after dissipating ∆ψm. Mean fluorescence intensity ratios evoked by the two excitation wavelengths (405 nm and 488 nm) at individual mitochondria in mutant hAPP axons with and without CCCP treatment were quantified and normalized to those of WT controls"
NCBI gene IDs:
"hAPP: 351"
Figure legend: "A,B Representative images (A) and quantitative analysis (B) showing aberrant accumulation of mitophagosomes labeled by LC3 and Cyto c antibodies in the hippocampal mossy fibers of snapin-cKO mouse brains. Note that white arrow-labeled mitophagosomes are not readily observed in WT mouse brains. The average number of mitophagosomes per section (320 μm × 320 μm) was quantified."
NCBI gene IDs:
"snapin: 20615"
Figure legend: "Representative blots showing aberrant mitophagic retention at synaptic terminals in snapin-mutant mouse brains. The levels of Rheb along with LC3-II, p62, and Rab7 are significantly elevated in the synaptosomal fractions (Syn) purified from snapin-deficient mouse brains, relative to those of WT littermate controls (red box). Equal amounts (10 μg) of Syn and post-nuclear supernatants (PNS ) from WT and snapin-mutant mouse brains were sequentially immunoblotted with antibodies against Snapin, mitophagic/autophagic proteins, and mitochondrial proteins VDAC and TOM20 on the same membrane after stripping between each antibody application. The purity of synaptosomal fractions was confirmed by relative enrichment of SYP and PSD95 and less abundance of GAPDH."
NCBI gene IDs:
"snapin: 20615"
Figure legend: "quantitative analysis The levels of Rheb along with LC3-II, p62, and Rab7 are significantly elevated in the synaptosomal fractions (Syn) purified from snapin-deficient mouse brains, relative to those of WT littermate controls WT and snapin-mutant mouse brains were sequentially immunoblotted with antibodies against TOM20 on the same membrane after stripping between each antibody application. The purity of synaptosomal fractions was confirmed by relative enrichment of SYP and PSD95 and less abundance of GAPDH. Data were quantified from five independent repeats."
NCBI gene IDs:
"snapin: 20615"
Figure legend: "E,F Representative blots (E) and quantitative analysis (F) showing mitophagic accumulation in the brains of snapin conditional KO (cKO) mice by crossing with Thy1-cre Tg mice. Note increased levels of Rheb along with LC3-II, p62, and Rab7 in mitochondrial (Mito) fractions isolated from snapin-deficient mouse brains, relative to those of WT littermate controls (red box). Data were quantified from five independent repeats. PNS: post-nuclear supernatants."
NCBI gene IDs:
"cre: 2777477
snapin: 20615
Thy1: 21838"
Figure legend: "G,H Representative TEM images (G) and quantitative analysis (H) showing abnormal presynaptic retention of mitophagosome-like structures in the hippocampal regions of snapin-deficient mouse brains. Mitophagosomes, indicated by black arrows, are not readily detected in WT mouse brains. Data were quantified from the total number of the imaging fields (n) indicated in parentheses (H)."
NCBI gene IDs:
"snapin: 20615"
Figure legend: "I,J Representative images (I) and quantitative analysis (J) showing that deletion of snapin in mouse brains induces synaptic damage. Note that the density of presynaptic terminals in the hippocampal mossy fibers revealed by SYP immunostaining is significantly reduced in snapin-mutant mice crossed with CamKII-cre Tg mice at the age of 8 months, relative to that of WT littermate controls."
NCBI gene IDs:
"CamKII: 12322
cre: 2777477
snapin: 20615"
Figure legend: "Representative dual-channel kymographs (A) and quantitative analysis (B showing that overexpression of Snapin, but not Snapin-L99K, a Snapin mutant deficient in dynein motor binding, reduces mitophagic accumulation within AD axons incubated with CCCP."
NCBI gene IDs:
"Snapin: 20615"
Figure legend: "Representative quantitative analysis showing that overexpression of Snapin, but not Snapin-L99K, a Snapin mutant deficient in dynein motor binding, enhances retrograde transport of mitophagosomes within AD axons incubated with CCCP."
NCBI gene IDs:
"Snapin: 20615"
Figure legend: "D Mean fluorescence intensity ratios evoked by 405 nm and 488 nm excitation wavelengths at individual mitochondria in mutant hAPP axons expressing Snapin or Snapin-L99K were quantified and normalized to those of mutant hAPP controls. Note that oxidatively stressed mitochondria are reduced within AD axons expressing Snapin after CCCP treatment."
NCBI gene IDs:
"hAPP: 351
Snapin: 20615"
Figure legend: "A Representative images showing gene delivery into the hippocampus of AD mice injected with AAV-mCherry or AAV-mCherry-Snapin. mCherry fluorescence indicated by white arrows is present in the soma and processes of transduced neurons in the hippocampal CA3 and dentate gyrus (DG) of mutant hAPP mouse brains. NeuN, a neuronal nuclear marker, indicates neurons."
NCBI gene IDs:
"mCherry: 
hAPP: 351
Snapin: 20615"
Figure legend: "Attenuation of mitophagic accumulation in the hippocampal mossy fibers of AD mice transduced with AAV-mCherry-Snapin. Note a marked reduction of mitophagosomes (white arrows) in mutant hAPP Tg mouse brains with elevated Snapin expression Cyto c*: color is converted from blue to red for better contrast"
NCBI gene IDs:
"mCherry: 
hAPP: 351
Snapin: 20615"
Figure legend: "Attenuation of mitophagic accumulation in the hippocampal mossy fibers of AD mice transduced with AAV-mCherry-Snapin. Note a marked reduction of mitophagosomes (white arrows) in mutant hAPP Tg mouse brains with elevated Snapin expression"
NCBI gene IDs:
"mCherry: 
hAPP: 351
Snapin: 20615"
Figure legend: "D,E Quantitative analysis (D) and representative images (E) showing increased density of presynaptic terminals in the hippocampal mossy fibers of AD mice infected with AAV-mCherry-Snapin. Blue indicates the signal of DAPI staining. The percentage area of SYP-labeled presynaptic terminals was quantified and normalized to that of control AD mice injected with AAV-mCherry (D). Data information: Data were quantified from a total number of 40-44 imaging slice sections of three pairs of mutant hAPP Tg mice with AAV injection."
NCBI gene IDs:
"mCherry: 
hAPP: 351
Snapin: 20615"
Figure legend: "mRNA expression by qRT-PCR of Rab5, Rab7a, Rab7b, and Rab9. Significance determined by one-way ANOVA, Tukey post hoc. Each data point represents the mean of triplicate technical replicates from a single mouse, n=3-4 mice in independent experiments. Error bars denote standard deviation."
NCBI gene IDs:
"Rab5: 271457
Rab7a: 19349
Rab7b: 226421
Rab9: 56382"
Figure legend: "ChIP-PCR on mouse stomach tissue at vehicle and 6 hour HDT. Chromatin probed with ATF3, Histone H3 antibodies, or a Rabbit IgG control. Also included is the 2% chromatin input and water to control for DNA contamination. Rab7b amplicon is from a conserved, putative ATF3-binding site in the first intron. Amplicon from Atf3 is a positive control from a previously characterized ATF3-binding site; negative control is from a site in Rab9a lacking putative ATF3 binding motifs."
NCBI gene IDs:
"Atf3: 11910
Rab7b: 226421
Rab9a: 56382"
Figure legend: "mRNA expression of ATF3 by qRT-PCR in control, ATF3 knockdown (KD), and ATF3 overexpression (OE) AGS cell lines generated using a PiggybacTM transposon system. Significance by one-way ANOVA, Dunnett post-hoc. Datapoint = mean of technical triplicates in a single mouse. n=3 mice. Error bars denote standard deviation. mRNA expression of RAB7B by qRT-PCR in AGS cell lines from 3A. Statistics and plotting as for panel A."
NCBI gene IDs:
"ATF3: 11910
RAB7B: 226421"
Figure legend: "Top - Immunofluorescence for RAB7 (red) and DAPI (blue, nuclei) in control, ATF3 knockdown (KD), and ATF3 overexpression (OE) AGS cells. Arrowhead indicates aggregated RAB7 vesicular formation similar to those observed in tissue. Bottom - Immunofluorescence for ATF3 (purple) and Cathepsin D (CTSD, green, lysosomes) in AGS cells. Scale bar, 20µm."
NCBI gene IDs:
"ATF3: 11910"
Figure legend: "Representative immunofluorescence images of gastric chief cells after vehicle or 24h HDT treatment stained for Pepsinogen C (PGC, red, granules) and MAP1LC3B (green, autophagy marker). Top row is wild-type, bottom row is Atf3−/−. Scale bar, 50μm. Stomach unit base outlined by dashed white line."
NCBI gene IDs:
"Atf3: 11910"
Figure legend: "Transmission electron micrograph images of zymogenic chief cells (ZCs) after 24h vehicle or HDT treatment in WT (top) and Atf3−/− mice (bottom). Scale bar, 1μm. Arrowheads indicate cell components being degraded. Quantification of degradation events per cell counted from EM images of ZCs after 24h HDT treatment in WT and Atf3−/−mice as shown in panel 3D. Each data point=total granules in a cell in a single TEM section with cells from 2 separate mice plotted together (data point colors indicate cells from a single mouse). Red line indicates mean across all the mice."
NCBI gene IDs:
"Atf3: 11910"
Figure legend: "Representative immunofluorescence images of gastric units after vehicle or 72h high-dose tamoxifen (HDT) treatment stained for GIF (red, granules), GSII (green, neck cells and SPEM marker), BRDU (white, proliferation) and DAPI (blue, nuclei). Top row is wild-type, bottom row is Atf3−/−. Yellow arrowheads point to areas of base dropout where ZCs have been lost. Scale bar, 50μm. Stomach units outlined by dashed white line."
NCBI gene IDs:
"Atf3: 11910"
Figure legend: "Representative immunohistochemistry of Cleaved-Caspase 3 in pancreatic acinar cells marking apoptosis in vehicle or 24h cerulein (CER) treatment. Top row is WT, bottom row is Atf3−/−. Scale bar, 50μm. Apoptosis quantified following 24h CER injury by the number of Cleaved-Caspase 3+ cells from a 40x field. Each data point is mean of positive cells in 4-5 40x fields in an individual mouse, n=3 mice per treatment group. Significance by unpaired two-tailed t-test. Error bars denote standard deviation."
NCBI gene IDs:
"Atf3: 11910"
Figure legend: "Representative immunohistochemistry of BrdU in pancreatic acinar cells marking proliferation in vehicle or 7 days of repeated cerulein injury. Top row is WT, bottom row is Atf3−/−. Scale bar, 50μm. Quantification of proliferation at peak ADM as counted by the number of BrdU+ cells from a 20x field following 7D cerulein treatment. Plotting and statistical analysis as per panel F."
NCBI gene IDs:
"Atf3: 11910"
Figure legend: "(A) mRNA expressions  of PINK1  in CSCs  and non-CSCs. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"PINK1: 65018"
Figure legend: "(Q) CSCs  transfected with PINK1  shRNA or the control were analysed for  sphere formation  and tumorgenicity. Mean±SEM from 6 experiments. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"PINK1: 65018"
Figure legend: "(S) CSCs   were transfected with PINK1  shRNA or control, and analysed for activated Notch1. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"PINK1: 65018"
Figure legend: "(T) CSCs  with or without transfection of Notch1  ICD were treated with Mdivi-1 (50 μM) and analyzed for sphere formation plus tumorgenicity. Mean±SEM from 6 experiments. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Notch1: 4851"
Figure legend: "(E) CSCs  with or without transfection of PINK1  shRNA were analysed for lysosomal mtDNA. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"PINK1: 65018"
Figure legend: "(L) CSCs  transfected with DNase II  expression vector or the control were analyzed for lysosomal DNA  plus MyD88  protein. Mean±SEM from 6 patients. (M) CSCs  transfected with  DNase II shRNA or the control were analyzed for lysosomal DNA  plus MyD88  protein. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"DNase II: 1777"
Figure legend: "(D) CSCs  were transfected with 2 different TLR9  shRNAs or the control, and analysed for  sphere formation and tumorgenicity. Mean±SEM from 6 experiments. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"TLR9: 54106"
Figure legend: "(E-F) CSCs  transfected with  DNase II expression vector, DNase II shRNA, or the control was analyzed for sphere formation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"DNase II: 1777"
Figure legend: "(A) CSCs  transfected with TLR9  shRNA or control were analysed for protein expressions  of activated Notch1  plus HES1. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"TLR9: 54106"
Figure legend: "(C) CSCs  transfected with  DNase II  expression vector or control were analyzed for activated Notch1 expression. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"DNase II: 1777"
Figure legend: "(F) CSCs  were transfected with 2 different Notch1  shRNAs or control, treated with CpG ODN  (10 μg/ml), and analyzed for sphere formation  plus tumorgenicity. Mean±SEM from 6 experiments. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Notch1: 4851"
Figure legend: "(D) CSCs  transfected with Notch1  shRNA or control shRNA were analysed for mitochondrial OCR. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Notch1: 4851"
Figure legend: "(F) CSCs  transfected with DNase II expression vector or control were analysed for mitochondrial OCR. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"DNase II: 1777"
Figure legend: "(C) CSCs  transfected with Notch1  shRNA or control were stimulated with  CpG ODN (10 μg/ml) and analyzed for phosphor-AMPKα. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method , **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001."
NCBI gene IDs:
"Notch1: 4851"
Figure legend: "(G) CSCs  were transfected with 2 different AMPKα  shRNA or the control, stimulated with  CpG ODN (10 μg/ml), and analysed for mitochondrial OCR. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method , **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001."
NCBI gene IDs:
"AMPKα: 5562///5563"
Figure legend: "(A) CSCs  transfected with or without Notch1  ICD were analyzed for AMP/ATP  ratio plus LKB1 phosphorylation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Notch1: 4851"
Figure legend: "(D) CSCs  transfected with LKB1  shRNA（right）or the control (left) were treated with CpG ODN (10 μg/ml) and analysed for AMPK phosphorylation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"LKB1: 6794"
Figure legend: "(E) CSCs  transfected with TAK1  shRNA or the control were treated with CpG ODN  (10 μg/ml) and analysed for AMPK phosphorylation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"TAK1: 7182"
Figure legend: "(F) CSCs  transfected with LAMTOR1  shRNA or the control were treated with CpG ODN (10 μg/ml) and analysed for AMPK phosphorylation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"LAMTOR1: 55004"
Figure legend: "(G) CSCs  transfected with AXIN1  shRNA or the control were treated with CpG ODN  (10 μg/ml) and analysed for AMPK phosphorylation. Mean±SEM from 6 patients. Data information: Paired t-test with Bonferroni method, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"AXIN1: 8312"
Figure legend: "G. RT-qPCR analyses of FACS-purified CD34+α6+ and CD34-α6+ cells from either 3C cultures or from freshly isolated epidermis show upregulation of HFSC identity in both freshly isolated and 3C-CD34+α6+ cells compared to freshly isolated CD34-α6+ progenitors. 3C- CD34-α6+ cells show intermediated expression (mean ±SEM; n=4; *p ≤ 0.05, ** p ≤ 0.01, Kruskal-Wallis)."
NCBI gene IDs:
"CD34: 12490"
Figure legend: "Labelling scheme and representative DNA fibers from U2OS cells transfected with siLuc or siChk1."
NCBI gene IDs:
"Luc: 
Chk1: 1111"
Figure legend: "Control (siLuc) and Chk1-depleted (siChk1) U20S cells were labeled with CldU and IdU for 10 and 30 min, respectively. Mean (+SEM) track lengths (top) and CldU/IdU ratios (bottom) are shown. Data come from 6 independent experiments and a total of 579 (siLuc) and 590 (siChk1) fibers were scored."
NCBI gene IDs:
"Luc: 
Chk1: 1111"