EMBO/SourceData huggingface dataset:
Figure legend: "Rescue of stxd77 by over expressing Octβ2R in ellipsoid body. From left to right mean±SEM: 399.6±11.49, n=50; 400.3±10.48, n=60; 349.9±14.77, n=48; 495.8±16.43, n=28; 470.1±13.04, n=44; 423.6±14.32, n=47; 548.6±8.707, n=50; 499.0±8.225, n=60; 490.7±16.26, n=48; 494.7±11.99, n=28; 480.2±14.64, n=44; 437.4±14.85, n=47."
NCBI gene IDs:
"Octβ2R: 41549
stx: 32005"
Figure legend: "Rescue of stxd77 by Pc RNAi in ellipsoid body. From left to right mean±SEM: 399.9±11.88, n=48; 409.8±11.50, n=49; 348.7±20.19, n=32; 495.8±16.43, n=28; 459.3±14.78, n=61; 407.5±15.66, n=40; 550.7±8.881, n=48; 516.0±11.76, n=49; 502.3±15.08, n=32; 494.7±11.99, n=28; 546.3±11.35, n=61; 514.2±18.94, n=40. Note that the same data is used for genotype stxd77;; EB1-Gal4 in panel F and G."
NCBI gene IDs:
"EB1: 35584
Gal4: 855828
Pc: 40358
stx: 32005"
Figure legend: "Quantitative RT-PCR of stx after OA treatment in w1118 control and Octβ1R, Octβ2R mutants, Octβ3R mutants. (From left to right mean±SEM: 1.000±0.0288, 0.781±0.0176, 1.477±0.043，1.403±0.0578, 1.157±0.0617, 0.881±0.0689, 1.149±0.0398, 0.953±0.0543, 1.017±0.0600, 1.090±0.0378, N=3)."
NCBI gene IDs:
"Octβ1R: 42652
Octβ2R: 41549
Octβ3R: 3885573
stx: 32005"
Figure legend: "Ellipsoid body knock down of Octβ2R results in sleep rebound increase. In response to OA, ellipsoid knock down of Octβ2R rescues control phenotype. Data represent mean±SEM: 13.80±2.439, n=31; 5.465±2.036, n=25; 19.55±2.275, n=27; 12.47±2.684, n=24; 30.03±3.428, n=25; 43.67±4.616, n=25."
NCBI gene IDs:
"Octβ2R: 41549"
Figure legend: "Sleep profile of PcXT109 mutant with or without OA treatment. In both control and PcXT109 mutant flies, the treatment of OA results in sleep decrease, n=16 (G). Quantifications of the decreasing amount showed that PcXT109 mutant fly loss significantly more sleep that control flies (From left to right mean±SEM: 13.90±1.397; 21.25±1.493, N=4) (H)."
NCBI gene IDs:
"Pc: 40358"
Figure legend: "Total sleep for Tβh mutant and control flies in video-based method (From left to right mean±SEM: 688.2±32.71, n=48; 123.2±11.68, n=46)."
NCBI gene IDs:
"Tβh: 31718"
Figure legend: "B-D'') Representative images showing that Hh accumulates on the surface of LDs in glial cells of the CB (yellow arrows) Glial cells are marked by repo-GAL4&gt;GFP and CB is circled in (B)."
NCBI gene IDs:
"GFP: 
GAL4: 855828
repo: 47285"
Figure legend: "K-L'') Hh-LD associations are observed in the cortex glia (yellow arrows, NP2222-GAL4&gt;mGFP)."
NCBI gene IDs:
"mGFP: 
NP2222: 
GAL4: 855828"
Figure legend: "A-D) Representative images showing that upon knockdown of Hh in cortex glial cells (NP2222-GAL4&gt;mGFP with UAS-dcr2), cortex glial membrane and overall Repo+ glial cell number are significantly reduced, quantified in (C) (n=7, 8 brain lobes) and (D) (n=7, 8 brain lobes), respectively. E-G) Hh knockdown in glia (repo-GAL4&gt;GFP) results in niche disruption and clustering of NBs (circled with yellow dashed line), as well as an increase in the percentage of NBs in M phase (pH3+), quantified in (E) (n=12, 10 brain lobes). "
NCBI gene IDs:
"mGFP: 
GFP: 
NP2222: 
dcr2: 36993
GAL4: 855828
Hh: 42737
repo: 47285"
Figure legend: "H-K) Representative images showing that Hh overexpression using pan-glial (repo-GAL4) and cortex-glial (NP2222-GAL4) drivers both result in a decrease in NB EdU index, quantified in (J) (n=16, 20 brain lobes) and (K) (n=11, 14 brain lobes), respectively."
NCBI gene IDs:
"GAL4: 855828
Hh: 42737
repo: 47285"
Figure legend: "L-M'') Representative images showing ci-lacZ is expressed in NBs (yellow arrows). (M-M'') are zoomed in images of (L)."
NCBI gene IDs:
"lacZ: 
ci: 43767"
Figure legend: "N-P) Overexpression of ciACT in NBs (dnab-GAL4) reduces EdU index, quantified in (P) (n=12, 10 brain lobes)."
NCBI gene IDs:
"ci: 43767
GAL4: 855828
dnab: 3346237"
Figure legend: "E-G) Lsd-2 knockdown in cortex glial cells (NP2222-GAL4) where hh is overexpressed effectively reduces LD number in CB (outlined in yellow dashed lines). H-K) Representative images showing that NB EdU index is rescued upon Lsd-2 knockdown in cortex glial cells (NP2222-GAL4) where hh is overexpressed, quantified in (K) (n=11, 14; 16, 14; 6, 14 brain lobes). The NP2222-GAL4&gt;w1118 vs hhOE columns depict the same data as Fig 2K. EdU+ NBs are circled with yellow, dashed lines. "
NCBI gene IDs:
"NP2222: 
GAL4: 855828
hh: 42737
Lsd-2: 32437"
Figure legend: "A-D') Representative images showing that pan-glial overexpression of htlACT, but not InRwt or EgfrACT causes an expansion of cortex glia that enwraps NBs. Glial cells are marked with repo-GAL4 &gt;GFP, and NBs are marked with Mira. (A', B', C' and D') are zoomed in images of (A, B, C and D), respectively."
NCBI gene IDs:
"GFP: 
Egfr: 37455
GAL4: 855828
htl: 42160
InR: 42549
repo: 47285"
Figure legend: "Representative images showing that cortex glial overexpression of htlACT but not InRwt or EgfrACT causes an increase in cortex glial membrane size (H) (n=10, 10 brain lobes) NP2222-GAL4&gt;mGFP is used to mark cortex glial membrane in (E-G)"
NCBI gene IDs:
"mGFP: 
NP2222: 
Egfr: 37455
GAL4: 855828
htl: 42160
InR: 42549"
Figure legend: "Representative images showing that cortex glial overexpression of EgfrACT to mark cortex glial membrane wrapper-GAL4&gt; is used in (I, J)."
NCBI gene IDs:
"Egfr: 37455
GAL4: 855828
wrapper: 37555"
Figure legend: "cortex glial overexpression of htlACT but not InRwt or EgfrACT causes an increase in glial cell (Repo+) numbers quantified in (K) (n=10, 10, 10; 7, 12 brain lobes)"
NCBI gene IDs:
"Egfr: 37455
htl: 42160
InR: 42549
Repo: 47285"
Figure legend: "L) Glial (repo-GAL4&gt;) overexpression of InRwt but not htlACT or EgfrACT significantly reduces the number of CB NBs (n= 10, 10, 8, 11 brain lobes)."
NCBI gene IDs:
"Egfr: 37455
GAL4: 855828
htl: 42160
InR: 42549
repo: 47285"
Figure legend: "M) Glial (repo-GAL4&gt;) overexpression of htlACT but not InRwt or EgfrACT significantly reduces the pH3 index of CB NBs"
NCBI gene IDs:
"Egfr: 37455
GAL4: 855828
htl: 42160
InR: 42549
repo: 47285"
Figure legend: "A-E) Representative images showing that both pan-glial (repo-GAL4&gt;) and cortex glial (NP2222-GAL4&gt;) htlACT overexpression significantly reduce NB EdU index, quantified in (E) (n=15 ,14; 10, 16 brain lobes). (A, B, C, D) are single sections with Mira and EdU staining, and (A', B' C', D') are Z-projection of the EdU staining."
NCBI gene IDs:
"NP2222: 
GAL4: 855828
htl: 42160
repo: 47285"
Figure legend: "pan-glial (repo-GAL4) htlACT overexpression lengthens NB cell cycle, quantified in (F) The cell cycle length is measured as the length between consecutive divisions."
NCBI gene IDs:
"GAL4: 855828
htl: 42160
repo: 47285"
Figure legend: "Representative still images from ex vivo CNS live imaging at 72ALH showing that pan-glial (repo-GAL4) htlACT overexpression lengthens NB cell cycle, (n= 25, 6 NBs imaged from 3 brains per genotype). NBs (Dpn::GFP, red; Histone RFP, grey) are circled with blue dashed lines."
NCBI gene IDs:
"GAL4: 855828
htl: 42160
repo: 47285"
Figure legend: "M-O) Representative images showing that the number of EdU+ neurons generated per NB is significantly reduced upon pan-glial overexpression of FGF (repo-LexA &gt; LexAop-htlACT; yellow arrows), quantified in (O) n= 94, 127 NB lineages imaged from 5 and 7 brain lobes, respectively). NB lineages are marked with dnab-gal4 &gt; GFP."
NCBI gene IDs:
"FGF: 
GFP: 
LexA: 
gal4: 855828
htl: 42160
dnab: 3346237
repo: 47285"
Figure legend: "A) Pan-glial (repo-GAL4) htlACT overexpression causes upregulation of hh, fasn1 and lsd2 transcripts (n = 3 biological replicates pooled from 20 brains for each genotype; for each biological replicate, we ran 3 technical replicates for each PCR reaction). The lipogenesis genes (acc, lipin), and lipolysis gene bmm transcripts are not significantly altered. We utilised rpl32 as a reference gene in these experiments, as it is not altered by htlACT overexpression. The data are represented by log2 fold change relative to the control (repo-GAL4&gt; w1118)."
NCBI gene IDs:
"rpl32: 
acc: 35761
bmm: 39611
fasn1: 33524
GAL4: 855828
hh: 42737
htl: 42160
lipin: 35790
lsd2: 32437
repo: 47285"
Figure legend: "C-G) Representative images showing that Hh staining normally localised to a ring like structure (yellow arrows), becomes delocalises to the glial cytoplasm upon htlACT overexpression, quantified in (E) (n=4, 6 brain lobes). Glial cells are marked with repo-GAL4&gt;GFP. (E-F') are zoomed in images of (C-D')."
NCBI gene IDs:
"GFP.: 
GAL4: 855828
htl: 42160
repo: 47285"
Figure legend: "H) Cortex glial (NP2222-GAL4&gt;) overexpression of two independent hh RNAis significantly rescue EdU incorporation defects caused by htlACT overexpression (n=10, 16; 7, 12; 14, 11 brain lobes)."
NCBI gene IDs:
"NP2222: 
GAL4: 855828
hh: 42737
htl: 42160"
Figure legend: "I) Knockdown of NB Hh signalling pathway (dnab-GAL4&gt; UAS-ciRNAi) rescues NB EdU incorporation defects induced by glial htlACT overexpression (repo-LexA&gt; LexAop-htlACT). Induction of ciRNAi in NBs alone increases NB EdU incorporation (n=8, 13; 10, 10; 30, 26; 12, 7 brain lobes)."
NCBI gene IDs:
"LexA: 
ci: 43767
GAL4: 855828
htl: 42160
dnab: 3346237
repo: 47285"
Figure legend: "J) The NB EdU incorporation defects due to cortex glial (NP2222-GAL4&gt;) overexpression of htlACT, is significantly rescued by overexpression of RNAis against fasn1 and lsd2, compared to corresponding control RNAis (n=10, 16; 25, 17; 14, 8 brain lobes)."
NCBI gene IDs:
"NP2222: 
fasn1: 33524
GAL4: 855828
htl: 42160
lsd2: 32437"
Figure legend: "B) Inhibition of palmitoylation (via two independent rasp RNAis) rescues NB EdU incorporation defects induced by cortex glial (NP2222-GAL4&gt;) htlACT overexpression, while knockdown of Rasp in cortex glial cells alone does not alter NB EdU index (n=10, 15; 10, 16; 18, 21; 26, 29 brain lobes)."
NCBI gene IDs:
"NP2222: 
GAL4: 855828
htl: 42160
rasp: 44098
Rasp: 44098"
Figure legend: "C-C') Representative images showing that Hh.N.EGFP (which cannot undergo cholesterol modification) are found as puncta on the surface of NBs (yellow arrows) when overexpressed in neighbouring cortex glial cells (NP2222-GAL4&gt;)."
NCBI gene IDs:
"NP2222: 
GAL4: 855828"
Figure legend: "Representative images showing that knockdown of Fasn1 in glial cells (repo-GAL4&gt;), where Hh.N.EGFP is overexpressed, significantly reduces the number of Hh.N.EGFP puncta on the surface of NBs (yellow arrows) n=38, 38, 57 NBs from 4, 4, 8 brain lobes, respectively)."
NCBI gene IDs:
"EGFP: 
Fasn1: 33524
GAL4: 855828
Hh: 42737
repo: 47285"
Figure legend: "knockdown of Fasn1 in glial cells (repo-GAL4&gt;), where Hh.N.EGFP is overexpressed, significantly reduces the number of Hh.N.EGFP puncta on the surface of NBs phenocopying the effect of Rasp knockdown, quantified in (G)"
NCBI gene IDs:
"EGFP: 
Fasn1: 33524
GAL4: 855828
Hh: 42737
Rasp: 44098
repo: 47285"
Figure legend: "H) Cortex glial (NP2222-GAL4&gt;) overexpression of Hh.N.EGFP significantly reduces NB EdU incorporation (n= 13,12 brain lobes)."
NCBI gene IDs:
"EGFP: 
NP2222: 
GAL4: 855828
Hh: 42737"
Figure legend: "I) Knockdown of Fasn1 in glial cells rescues NB EdU incorporation defects, caused by glial Hh.N.EGFP overexpression, quantified in (I) (n=15, 13 brain lobes)."
NCBI gene IDs:
"EGFP: 
Fasn1: 33524
Hh: 42737"
Figure legend: "(A) Representative EPSP and mEPSP electrophysiological traces from larval NMJs at Muscle 6 in which a motoneuron specific driver was used to overexpress either a control transgene (DVGlut-GAL4 &gt; UAS-RFP), dNmnat (DVGlut-Gal4 &gt; UAS-dNmnat), or Wnd (DVGlut-Gal4 &gt; UAS-Wnd)."
NCBI gene IDs:
"RFP: 
GAL4: 855828
Gal4: 855828
dNmnat: 42987
DVGlut: 33427
Wnd: 40143"
Figure legend: "(B) Quantification of mean (±SEM) EPSP amplitudes, in which 75 consecutive evoked events were averaged per cell, and then cell amplitudes were averaged per genotype. [UAS-RFP n = 13 cells, UAS-dNmnat n = 14 cells, UAS-Wnd n= 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=36, F=12.66, p&lt;0.0001. UAS-RFP vs UAS-dNmnat p &lt; 0.0001 (****), UAS-RFP vs UAS-Wnd p = 0.0093 (**)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987
Wnd: 40143"
Figure legend: "]. (C) Quantification of mean (±SEM) mEPSP amplitudes, in which 75 consecutive spontaneous events were averaged per cell, and then cell amplitudes were averaged per genotype. [UAS-RFP n = 15 cells, UAS-dNmnat n = 14 cells, UAS-Wnd n= 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=38, F=11.66, p = 0.0001. UAS-RFP vs UAS-dNmnat p=0.994 (NS), UAS-RFP vs UAS-Wnd p = 0.0003 (***)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987
Wnd: 40143"
Figure legend: "(D) Quantification of quantal content, which was calculated individually per cell by dividing the mean EPSP amplitude by the mean mEPSP amplitude, and then averaged per genotype. [UAS-RFP n = 15 cells, UAS-dNmnat n = 14 cells, UAS-Wnd n= 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=38, F=9.286, p = 0.0006. UAS-RFP vs UAS-dNmnat p=0.0015 (**), UAS-RFP vs UAS-Wnd p = 0.0003 (***)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987
Wnd: 40143"
Figure legend: "(F) Quantification of the mean (±SEM) number of DVGlut+ boutons per muscle 4 NMJ in each genotype. [UAS-RFP n = 23, UAS-dNmnat n = 21, UAS-Wnd n= 20 NMJs. 1-way ANOVA w/Tukey's multiple comparisons, DF=63, F=68.31, p &lt; 0.0001. UAS-RFP vs UAS-dNmnat p=0.9818 (NS), UAS-RFP vs UAS-Wnd p &lt; 0.0001(****)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987
Wnd: 40143"
Figure legend: "(A) Representative EPSP and mEPSP physiological traces from larval NMJs at Muscle 6. Recordings were taken from WT control larvae (Elav-Gal4&gt;UAS-RFP), hiw mutants driving a control transgene (hiwND8;Elav-Gal4 &gt; UAS-RFP), and hiw mutants expressing RNAis to knockdown either dNmnat (hiwND8;Elav-Gal4 &gt; UAS-dNmnat-RNAi [#29402] ) or Wnd (hiwND8;Elav-Gal4 &gt; UAS-Wnd-RNAi [#25396])."
NCBI gene IDs:
"RFP: 
Elav: 31000
Gal4: 855828
hiw: 32429
dNmnat: 42987
Wnd: 40143"
Figure legend: "(B) Quantification of mean (±SEM) EPSP amplitudes, in which 75 consecutive evoked events were averaged per cell, and then cell amplitudes were averaged per genotype. [WT n = 10 cells, hiw n = 10 cells, hiw &gt; Wnd-RNAi n= 10 cells, hiw &gt; dNmnat-RNAi n = 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=39, F=14.02, p&lt;0.0001. WT vs hiw p = 0.0012 (**), WT vs hiw &gt; Wnd-RNAi p &lt; 0.0001 (****), WT vs hiw &gt; dNmnat-RNAi p = 0.9880 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987
Wnd: 40143"
Figure legend: "(C) Quantification of quantal content (±SEM). [WT n = 9 cells, hiw n = 9 cells, hiw &gt; Wnd-RNAi n= 10 cells, hiw &gt; dNmnat-RNAi n = 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=38, F= 2.508, p&lt;0.0001. WT vs hiw p = 0.0361 (*), WT vs hiw &gt; Wnd-RNAi p = 0.0001 (***), WT vs hiw &gt; dNmnat-RNAi p = 0.9889 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987
Wnd: 40143"
Figure legend: "(E) Quantification of the mean (±SEM) number of DVGlut+ boutons per muscle 4 NMJ in each genotype. [WT n = 23, hiw n = 21 , hiw &gt; Wnd-RNAi n= 16, hiw &gt; dNmnat-RNAi n=19. 1-way ANOVA w/Tukey's multiple comparisons, DF=78, F=169.1, p &lt; 0.0001. WT vs hiw p &lt; 0.0001 (****), WT vs hiw &gt; Wnd-RNAi p = 0.9988 (NS), WT vs hiw &gt; dNmnat-RNAi p &lt; 0.0001 (****), hiw vs hiw &gt; dNmnat-RNAi p = 0.9695 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987
Wnd: 40143"
Figure legend: "(A) Representative images of immunostained larval NMJs and active zones from WT, hiw mutant, and hiw mutant driving an RNAi against dNmnat [#29402]. In the top 3 panels, greyscale images of entire terminal were acquired by staining for nerve marker HRP. Presynaptic Brp puncta (green) and postsynaptic dGluRIII clusters (magenta) were imaged to quantify N in each genotype."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987"
Figure legend: "(B) Quantification of NMJ synaptic terminal surface area in each genotype. [WT n = 14, hiw n = 12 , hiw &gt; dNmnat-RNAi n=12. 1-way ANOVA w/Tukey's multiple comparisons, DF=37, F=22.427, p &lt; 0.0001. WT vs hiw p &lt; 0.0001 (****), WT vs hiw &gt; dNmnat-RNAi p &lt; 0.0001 (****), hiw vs hiw &gt; dNmnat-RNAi p = 0.2907 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987"
Figure legend: "(C) Quantification of BRP puncta per NMJ [WT n = 14, hiw n = 12, hiw &gt; dNmnat-RNAi n=12. 1-way ANOVA w/Tukey's multiple comparisons, DF=37, F=2.854, p = 0.0711. WT vs hiw p = 0.0573 (NS), WT vs hiw &gt; dNmnat-RNAi p = 0.4529 (NS), hiw vs hiw &gt; dNmnat-RNAi p = 0.5030 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987"
Figure legend: "(D) Quantification of percentage of Brp puncta that were apposed to a dGluRIII clusters at each NMJ [WT n = 14, hiw n = 12, hiw &gt; dNmnat-RNAi n=12. 1-way ANOVA w/Tukey's multiple comparisons, DF=37, F=2.093, p = 0.4716. WT vs hiw p = 0.2168 (NS), WT vs hiw &gt; dNmnat-RNAi p = 0.1882 (NS), hiw vs hiw &gt; dNmnat-RNAi p = 0.9966 (NS)]."
NCBI gene IDs:
"hiw: 32429
dNmnat: 42987"
Figure legend: "(E) Representative electrophysiological traces from a synaptic facilitation experiment in which trains of 5 pulses were used to evoke consecutive action potentials at 100 ms ISI. Genotypes for these experiments were: WT (elav&gt;UAS-RFP), hiw mutant (hiw; elav&gt;UAS-RFP), or hiw depleted of dNmnat (hiw; elav&gt;dNmnat-RNAi). (F) Quantification of the facilitation index, in which the amplitude of the 5th pulse is divided by the amplitude of the 1st pulse. [100 ms ISI: WT n=11, hiw n= 9, hiw&gt;dNmnat-RNAi n= 12. 1-way ANOVA w/Tukey's multiple comparisons, DF = 31, F= 7.546, p =0.0023. WT vs hiw p =0.0020 (**), hiw vs hiw&gt; dNmnat-RNAi p= 0.0229 (*), WT vs hiw&gt; dNmnat-RNAi p= 0.5141 (NS). 50 ms ISI: WT n=11, hiw n= 9, hiw&gt;dNmnat-RNAi n= 12. 1-way ANOVA w/Tukey's multiple comparisons, DF = 31, F= 5.587, p =0.0089. WT vs hiw p =0.0129 (*), hiw vs hiw&gt; dNmnat-RNAi p= 0.0217 (*), WT vs hiw&gt; dNmnat-RNAi p= 0.9546 (NS).]"
NCBI gene IDs:
"RFP: 
elav: 31000
hiw: 32429
dNmnat: 42987"
Figure legend: "(A) Representative images of NMJ synaptic terminals in control (DVGlut-Gal4 &gt; UAS-HA-dNmnat) and hiw mutant (hiwND9,DVGlut-Gal4 &gt; UAS-HA-dNmnat) larvae. NMJs at larval muscle 4 were identified using HRP (blue in top panel) immunostaining. Insets highlight active zones within single boutons, which were identified by staining for the presynaptic active zone protein Brp (red). An anti-HA antibody was used to stain for an HA-tagged dNmnat (green) transgene to measure protein levels in control and hiw mutant active zones."
NCBI gene IDs:
"HA: 
Gal4: 855828
hiw: 32429
dNmnat: 42987
DVGlut: 33427"
Figure legend: "(B) Quantification of HA-dNmnat levels across the entire NMJ synaptic terminal, normalized to total terminal area and presented as fold change over WT. [WT > HA-dNmnat n = 10 terminals, hiw > HA-dNmnat n = 10 terminals. Unpaired T-test, DF = 18, p = 0.0028 (**)]."
NCBI gene IDs:
"HA: 
hiw: 32429
dNmnat: 42987"
Figure legend: "(C) Quantification of HA-dNmnat levels per unit area in WT and hiw for synaptic terminals excluding active zones (herein referred to as \"terminals\") versus active zones (AZs) only. [WT n = 10 terminals, hiw n = 9 terminals, each parsed into terminal minus AZs and AZs only. 2-way ANOVA with Tukey's multiple comparisons, DF = 34, p = 0.0005 for row factor (terminal versus AZs) and p &lt; 0.0001 for column factor (WT vs hiw). WT terminal vs hiw terminal p = 0.9830 (NS). Hiw terminal vs hiw AZs p &lt; 0.0001 (****). WT AZs vs hiw AZs p &lt; 0.0001 (****).]"
NCBI gene IDs:
"hiw: 32429
Hiw: 32429"
Figure legend: "A) Representative electron micrographs of entire boutons in wild type, hiw;wnd, and hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi NMJs."
NCBI gene IDs:
"Elav: 31000
GAL4: 855828
hiw: 32429
dNmnat: 42987
wnd: 40143"
Figure legend: "B) High magnification electron micrographs showing representative &quot;T-bar&quot; structures at active zones in NMJs of the indicated genotypes. Red arrows denote the beginning and end of the T-bar top, demonstrating changes in T-bar width in the genotypes tested. As illustrated, a significant reduction in T-bar size is observed in hiw;wnd, and this defect is suppressed in hiw;wnd, Elav-GAL4 > dNmnat RNAi."
NCBI gene IDs:
"Elav: 31000
GAL4: 855828
hiw: 32429
dNmnat: 42987
wnd: 40143"
Figure legend: "C) Quantification of mean T-bar width [WT n = 90, hiw;wnd n = 55, hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi n=81. 1-way ANOVA w/Tukey's multiple comparisons, DF=225, F=13.829, p &lt; 0.0001. WT vs hiw;wnd p &lt; 0.0001 (****), WT vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi p = 0.9318 (NS), hiw;wnd vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi p &lt; 0.0001 (****)]. (D) Analysis of cumulative frequency distribution of T-bar width shows a left shift in distribution in hiw;wnd but no change in hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi compared with WT NMJs [WT vs hiw;wnd K-S test, D=0.5636, p &lt; 0.0001 (****), WT vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi K-S test, D=0.1815, p = 0.1240 (NS), hiw;wnd vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi K-S test, D=0.4121, p &lt; 0.0001 (****)]."
NCBI gene IDs:
"Elav: 31000
GAL4: 855828
hiw: 32429
dNmnat: 42987
wnd: 40143"
Figure legend: "]. (E) Quantification of mean T-bar height [WT n = 90, hiw;wnd n = 55, hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi n=81. 1-way ANOVA w/Tukey's multiple comparisons, DF=225, F=10.472, p &lt; 0.0001. WT vs hiw;wnd p = 0.0008 (***), WT vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi p = 0.6694 (NS), hiw;wnd vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi p &lt; 0.0001 (****)] (F) Analysis of cumulative frequency distribution of T-bar height shows a left shift in distribution in hiw;wnd but no change in hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi compared with WT NMJs [WT vs hiw;wnd K-S test, D=0.3343, p = 0.001 (***), WT vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi K-S test, D=0.1012, p = 0.7748 (NS), hiw;wnd vs hiw;wnd, Elav-GAL4 &gt; dNmnat RNAi K-S test, D=0.3484, p = 0.0008 (***)]."
NCBI gene IDs:
"Elav: 31000
GAL4: 855828
hiw: 32429
dNmnat: 42987
wnd: 40143"
Figure legend: "(B) Representative EPSP and mEPSP electrophysiological traces from larval NMJs at Muscle 6 in which a motoneuron specific driver was used to overexpress either a control transgene (DVGlut-GAL4 &gt; UAS-RFP), or one of the catalytic dNmnat mutants (DVGlut-Gal4 &gt; UAS-WR-dNmnat, DVGlut-Gal4 &gt; UAS-H30A-dNmnat)."
NCBI gene IDs:
"RFP: 
GAL4: 855828
Gal4: 855828
dNmnat: 42987
DVGlut: 33427"
Figure legend: "(C) Quantification of mean (±SEM) EPSP amplitudes, in which 75 consecutive evoked events were averaged per cell, and then cell amplitudes were averaged per genotype. [UAS-RFP n = 8 cells, UAS-WR-dNmnat n = 7 cells, UAS-H30A-dNmnat n= 7 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=21, F=0.8596, p = 0.4383. UAS-RFP vs UAS-WR-dNmnat p = 0.8810 (NS), UAS-RFP vs UAS-H30A-dNmnat p = 0.9997 (NS)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987"
Figure legend: "(D) Quantification of quantal content (±SEM) [UAS-RFP n = 8 cells, UAS-WR-dNmnat n = 7 cells, UAS-H30A-dNmnat n= 7 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=21, F=1.154, p = 0.3365. UAS-RFP vs UAS-WR-dNmnat p = 0.8696 (NS), UAS-RFP vs UAS-H30A-dNmnat p = 0.9697 (NS)]."
NCBI gene IDs:
"RFP: 
dNmnat: 42987"
Figure legend: "]. (E) Representative EPSP and mEPSP electrophysiological traces from larval NMJs at Muscle 6. Recordings were taken from WT control larvae (Elav-Gal4>UAS-RFP), hiw mutants driving a control transgene (hiwND8;Elav-Gal4 > UAS-RFP), and hiw mutants expressing an RNAi to knockdown NAD+syn (hiwND8;Elav-Gal4 > UAS-NAD+syn-RNAi)."
NCBI gene IDs:
"RFP: 
Elav: 31000
Gal4: 855828
hiw: 32429
NAD+syn: 32328"
Figure legend: "(F) Quantification of mean (±SEM) EPSP amplitudes, in which 75 consecutive evoked events were averaged per cell, and then cell amplitudes were averaged per genotype. [WT n = 10 cells, hiw n = 10 cells, hiw &gt; NADsyn-RNAi n = 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=29, F=14.02, p&lt;0.0001. WT vs hiw p &lt;0.0001 (****), WT vs hiw &gt; NADsyn-RNAi p = 0.5147 (NS)]."
NCBI gene IDs:
"hiw: 32429
NADsyn: 32328"
Figure legend: "]. (G) Quantification of quantal content. [WT n = 10 cells, hiw n = 10 cells, hiw &gt; NADsyn-RNAi n = 10 cells. 1-way ANOVA w/Tukey's multiple comparisons, DF=29, F= 6.635, p = 0.0047. WT vs hiw p = 0.0063 (**), WT vs hiw &gt; NADsyn-RNAi p = 0.8942 (NS)]."
NCBI gene IDs:
"hiw: 32429
NADsyn: 32328"