EMBO/SourceData huggingface dataset:
Figure legend: "IFM images of KIF13A-YFP transfected control and Rab22A-, BLOC-1Mu-, BLOC-2HPS6- knockdown HeLa cells. Cells were stained for AP-1 (γ) or internalized with Tf-Alexa fluor 594 arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs respectively. Scale bars: 10 μm"
NCBI gene IDs:
"YFP: 
BLOC-1Mu: 63915
BLOC-2HPS6: 79803
KIF13A: 63971
Rab22A: 57403"
Figure legend: "Immunoblotting analysis of proteins in control and Rab22A-, BLOC-1-, BLOC-2-depleted HeLa cells. *, non-specific bands. Protein band intensities were quantified and indicated on the gels."
NCBI gene IDs:
"BLOC-2: 79803
BLOC-1: 63915
Rab22A: 57403"
Figure legend: "IFM images of mCherry-Rab22A and KIF13A-YFP cotransfected control and BLOC-1Mu-, BLOC-2HPS6- knockdown HeLa cells ), arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs respectively. Scale bars: 10 μm"
NCBI gene IDs:
"mCherry: 
YFP: 
BLOC-1Mu: 63915
BLOC-2HPS6: 79803
KIF13A: 63971
Rab22A: 19334"
Figure legend: "BF and IFM images of control and Rab22A-depleted melanocytes. The colocalization coefficient (r, in mean±SEM) between the two markers indicated separately. Nuclei are stained with Hoechst33258 ), arrows indicate the melanocyte pigmentation and arrowheads point to the localization of TYRP1/GFP-Rab22A. Scale bars: 10 μm"
NCBI gene IDs:
"Rab22A: 19334"
Figure legend: "Graph represents the quantified melanin content in control sh, Rab22A sh and BLOC-1- melanocytes. n=3. The fold-change in melanin content (mean±SEM) indicated separately"
NCBI gene IDs:
"BLOC-1: 17828
Rab22A: 19334"
Figure legend: "Immunoblotting analysis of proteins in Control sh and Rab22A sh melanocytes ), protein band intensities were quantified and indicated on the gels"
NCBI gene IDs:
"Rab22A: 19334"
Figure legend: "EM of control and Rab22A-knockdown MNT-1 melanocytes. Arrowheads indicate PMEL fibrils. Scale bars: 1 μm. I, II, III, IV: stages of melanosomes. M: mitochondria. Magnified view of insets are shown separately and they emphasize the stage I melanosomes in both conditions. Note: the presence of hybrid stage I/II melanosomes in Rab22A-knockdown cells. Insets (b)-(d) and (g)-(h) are from different cells of respective condition. Scale bars: 200 nm."
NCBI gene IDs:
"Rab22A: 57403"
Figure legend: "BF and IFM images of GFP-Rab22A transfected BLOC-1-(Mu) melanocytes ), arrows indicate the melanocyte pigmentation and arrowheads point to the localization of TYRP1/GFP-Rab22A. Scale bars: 10 μm"
NCBI gene IDs:
"GFP: 
BLOC-1-(Mu): 17828
Rab22A: 19334"
Figure legend: "Immunoblotting analysis of proteins in BLOC-1- and BLOC-1R melanocytes protein band intensities were quantified and indicated on the gels"
NCBI gene IDs:
"BLOC-1: 17828"
Figure legend: "Subcellular membrane fractionation of control and Rab22A-, BLOC-1-, BLOC-2-knockdown HeLa cells and probed the fractions for pallidin, dysbindin and muted. Red colored box emphasizes BLOC-1 membrane association in the respective cell types."
NCBI gene IDs:
"BLOC-2: 79803
BLOC-1: 63915
Rab22A: 57403"
Figure legend: "Pull-down of His-Rab22AWT using HeL lysate the bead bound His-Rab22A/His-KIF13A domains were shown as coomassie stained gel separatel"
NCBI gene IDs:
"Rab22A: 19334"
Figure legend: "Pull-down of His-Rab22AWT usin melanocyte (E) lysate. In E, the beads were preloaded with GTPγS or GDP the bead bound His-Rab22A/His-KIF13A domains were shown as coomassie stained gel separatel"
NCBI gene IDs:
"Rab22A: 19334"
Figure legend: "Pull-down of different His-KIF13A domains using HeLa cell lysate the bead bound His-Rab22A/His-KIF13A domains were shown as coomassie stained gel separatel"
NCBI gene IDs:
"KIF13A: 63971"
Figure legend: "(A) Abnormal distribution of Cux1-positive neurons in CAMDI-KO mice. Expression of Cux1 in the somatosensory cortex was compared between P2 wild-type (WT) and CAMDI-KO (KO) mice. Scale bar, 100 μm.(B) Quantification of the number of Cux1-positive neurons. Note the abnormal distribution of neurons in deep cortical layers of CAMDI-KO mice. n = 3 mice/genotype (WT = 788 cells, KO = 2,139 cells). *, p&lt;0.05, **, p&lt;0.01, ***, p&lt;0.001, Two-way ANOVA followed by Scheffe's post-hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(C) Delay in neuronal migration by CAMDI deletion. Coronal sections through the somatosensory cortex of P21 WT and CAMDI-KO mice were analyzed following in utero electroporation of EGFP plasmid at E14.5. Scale bar, 100 μm.(D) Quantification of the number of EGFP-positive neurons. Note the abnormal distribution of neurons in lower cortical layers of CAMDI-KO mice. n = 3 for WT mice (546 cells), n = 4 for KO mice (680 cells). *, p&lt;0.05, ***, p&lt;0.001. Two-way ANOVA followed by Scheffe's post-hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(E) Aberrant axonal projection and terminal arbors by CAMDI deletion. Coronal sections through the somatosensory cortex of P21 WT and CAMDI-KO mice were analyzed following in utero electroporation of EGFP plasmid at E14.5. Representative pictures show an aberrant axonal projection of corticostriatal neurons in CAMDI-KO mice. Sections were stained with Hoechst 33258 and anti-EGFP antibody. Scale bar, 1 mm.(F) Quantification of the striatal intensity. n = 3 mice/genotype. ***, p&lt;0.001, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(A, B) Representative traces from adult control (WT) and CAMDI-KO (KO) in open field test. Locomotor activity (A) and distance traveled (B) for 15 min. n = 8 for WT mice, n = 7 for KO mice. *, p&lt;0.05, One-way ANOVA with Bonferroni's post hoc test (F(1, 13) = 5.53). Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(C, D) N-terminal region of CAMDI recognizes the second HDAC domain of HDAC6. SH-SY5Y cells were co-transfected with HDAC6-HA and each FLAG-CAMDI fragment (C) (fragment 1, 1-250; 2, 251-500; 3, 501-750; 4, 751-1000; 5, 1001-1451 amino acids) or co-transfected with FLAG-CAMDI and each HDAC6-HA fragment (D) (fragment 1 (DD1), 1-440; 2 (DD2), 441-840; 3, 841-1215 amino acids). Co-IP assay was performed with the indicated antibodies. n=3 independent experiments. **, p&lt;0.01, ***, p&lt;0.001, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134
HDAC6: 15185"
Figure legend: "(A) CAMDI and CAMDI Fragment 1 overexpression enhanced α-tubulin acetylation. HeLa cells overexpressing FLAG-CAMDI and FLAG-CAMDI frag.1 were immunostained with anti-FLAG or anti-Ac-tubulin antibodies. Ratio line-scans along the line (from x- to y-axis) from images. Scale bar, 10 μm."
NCBI gene IDs:
"CAMDI: 311134"
Figure legend: "(B-C) CAMDI blocked HDAC6-mediated α-tubulin deacetylation. (C) CAMDI N-terminal domain alone increased Ac-tubulin level. n=3 independent experiments. **, p&lt;0.01, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134"
Figure legend: "(D) Deletion of CAMDI N-terminal domain (1-250 amino acids) failed to inhibit HDAC6. n = 3 independent experiments. N.S., not significant. Two-way ANOVA followed by Scheffe's post-hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134
HDAC6: 15185"
Figure legend: "(A) Effect of HDAC6 over-expression or CAMDI co-expression on the level of acetylated α-tubulin in the centrosome. HeLa cells co-transfected with Centrin2-EGFP with/without HDAC6-HA and FLAG-CAMDI were immunostained with anti-EGFP (green) and anti-acetylated α-tubulin antibodies (red). Scale bar, 1 μm.(B) Quantification of intensity of acetylated α-tubulin at centrosome. n = 25, 30, 30 cells. *, p&lt;0.05, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134
HDAC6: 15185"
Figure legend: "(C) Effects of HDAC6 overexpression or CAMDI co-expression on the centrosomal localization of γ-tubulin. HeLa cells co-transfected with Centrin2-EGFP with/without HDAC6-HA and FLAG-CAMDI were immunostained with anti-EGFP (green) and anti-γ-tubulin antibodies (red). DNA was stained with Hoechst 33258. Scale bar, 1 μm.(D) Quantification of intensity of γ-tubulin at centrosome. n = 39, 16, 17 cells. *, p&lt;0.05, **, p&lt;0.01, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134
HDAC6: 15185"
Figure legend: "(E) CAMDI stabilizes centrosomal γ-tubulin through HDAC6 inhibition. Centrosomal fraction was isolated from cell lysates of SH-SY5Y cells expressing each construct, and γ-tubulin level was assessed by immunoblot analysis. n = 3 independent experiments. **, p&lt;0.01, One-way ANOVA followed with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 311134
HDAC6: 15185"
Figure legend: "(F) Effects of CAMDI knockdown on the centrosomal localization of γ-tubulin in vivo.Coronal sections through the somatosensory cortex of E17.5 were analyzed following in utero electroporation of EGFP and CAMDI-sh plasmid at E14.5. The sections were immunostained with anti-EGFP (green) and anti-γ-tubulin antibodies (red). Representative images of EGFP at Cortical Plateneuron expressing CAMDI-sh stained for endogenous γ-tubulin and with Hoechst (blue). Z-stack number 6 indicates centrosome of EGFP-positive CAMDI-sh-expressing neuron. Z-stack number 19 indicates centrosome of a nearby non-electroporated neuron. Quantification of γ-tubulin intensity. EGFP (-) = 12 cells, EGFP (+) (CAMDI-sh) = 12 cells. *, p&lt;0.05, One-way ANOVA with Bonferroni's post hoc test. Data are presented as mean ± SEM. Scale bar, 5 μm."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(G) Reduced γ-tubulin in the centrosome fraction of CAMDI-KO brain. The centrosomal fraction was isolated from brain lysates of WT and CAMDI-KO mice and γ-tubulin level was assessed by immunoblot analysis. n = 3 independent experiments. **, p&lt;0.01, One-way ANOVA followed with Bonferroni's post hoc test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(A) Rescue of a delayed cortical migration in CAMDI-KO mice by Tubastatin A. Immunohistochemical analysis of Cux1 expression in the somatosensory cortex at P2 after intraperitoneal injections of Tubastatin A into pregnant CAMDI-KO mice from E12.5-17.5. Scale bar, 100 μm.(B) Quantification of the number of Cux1-positive neurons. Note the abnormal distribution of neurons in deep cortical layers of CAMDI-KO mice. n = 3 mice/genotype (WT (vehicle) = 3,628 cells, KO (vehicle) = 2,816 cells, WT (Tubastatin A) = 1,847 cells, KO (Tubastatin A) = 2,364 cells). *, p&lt;0.05, **, p&lt;0.01, Two-way ANOVA followed by Tukey HSD test. Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "(A) Representative traces from control (WT) and CAMDI-KO (KO) mice in the open field test at P21. Sample traces of locomotor activity were shown.(B) Distance traveled for 10 min. n = 13 for WT (vehicle) mice, n = 13 for KO (vehicle) mice, n = 5 for WT (Tubastatin A) mice, n = 16 for KO (Tubastatin A) mice. **, p&lt;0.01, ***, p&lt;0.001. Two-way ANOVA followed by Scheffe's post-hoc test (main effect of genotype F(1, 43)=6.25, main effect of drug F(1, 43)=2.14, interaction F(1, 43)=12.68). Data are presented as mean ± SEM."
NCBI gene IDs:
"CAMDI: 545428"
Figure legend: "Immunoprecipitation  of SATB2  from mouse neonatal cortical  lysates. LEMD2  was detected by Western blotting in the SATB2-immunoprecipitate  from control but not SATB2-deficient cortical  lysate. The equal input of total protein from control and SATB2-deficient cortical  lysates was controlled by ERK2  detection. Representative images of the immunoblots  are shown; I (Input), F (Flow-through), B (Beads)."
NCBI gene IDs:
"SATB2: 212712"
Figure legend: "Immunoprecipitations  using anti-V5-tag antibody from lysates of HeLa cells transfected with expression plasmids encoding V5-tagged full-length SATB2  (Satb21-733) and EGFP  as control. The equal input of total protein was controlled by GAPDH  detection. Representative images of the immunoblots  are shown; I (Input), F (Flow‐through), B (Beads), M (Molecular weight marker)."
NCBI gene IDs:
"EGFP: 
SATB2: 
Satb2: 
V5: "
Figure legend: "The CUT-like domain of SATB2  is required for the interaction  with LEMD2. Immunoprecipitations  using anti-V5-tag antibody from lysates of HeLa  cells transfected with  V5-tagged Satb21-247 and Satb21-157 deletion mutants. LEMD2  and BAF  were detected only in immunoprecipitates  from HeLa  cells transfected with the deletion mutant containing the CUT-like domain (Satb21-247). The equal input of total protein was controlled by GAPDH  detection. Representative images of the immunoblots  are shown; I (Input), F (Flow‐through), B (Beads), M (Molecular weight marker)."
NCBI gene IDs:
"Satb2: 
V5: "
Figure legend: "CUT1 and CUT2 domains are required for the interaction  with LEMD2. Immunoprecipitations  using anti-V5-tag antibody from HeLa  cells transfected with  V5-tagged Satb2346-733 and  Satb2616-733 deletion mutants. LEMD2  and BAF  were detected only in immunoprecipitates  from HeLa  cells expressing the CUT1 and CUT2 containing deletion mutant (Satb2346-733)."
NCBI gene IDs:
"Satb2: 
V5: "
Figure legend: "Activity-induced formation of nuclear  infoldings is abolished in SATB2-defficient hippocampal neurons. Bic-induced AP bursting for 1 h caused a significant increase in the percentage of infolded nuclei  in DIV10 hippocampal  cultures derived from Satb2flx/flx mice  but not from  Satb2flx/flx::Nes-Cre mice  (Satb2NesCre) (n = 6 independent primary cultures, two-way ANOVA, F1,20 = 9.51, significant interaction p = 0.0058, simple main effects analysis, Satb2flx/flx cultures, Bic-treated vs untreated p = 0.0000043, Satb2NesCre cultures, Bic-treated vs untreated p &gt; 0.05, adjustment for multiple comparisons: Bonferroni, number of analyzed nuclei: Satb2flx/flx cultures, untreated - 680, Satb2flx/flx cultures, Bic-treated - 711, Satb2NesCre, untreated - 755, Satb2NesCre, Bic-treated - 720). Data are presented as mean ± SEM, ***p &lt; 0.001."
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
SATB2: 212712
Satb2: 212712"
Figure legend: "Activity-induced formation of nuclear  infoldings is impaired in  SATB2-deficient  cortical neurons. DIV14 cortical  cultures form Satb2flx/flx and Satb2NesCre mice  were silenced with NBQX  for 1 h followed by stimulation with Bic  for 1 h. The percentage of infolded nuclei  following AP bursting was increased in control Satb2flx/flx cultures but not in SATB2-deficient cultures (n = 3-4 independent primary cultures, two-way ANOVA, F1,10 = 39.124, significant interaction p = 0.000094, simple main effects analysis, Satb2flx/flx cultures: Bic-treated vs NBQX-treated p = 7.66E-07, Satb2NesCre cultures: Bic-treated vs NBQX-treated p &gt; 0.05, adjustment for multiple comparisons: Bonferroni, number of analyzed nuclei: Satb2flx/flx cultures, NBQX  - 606, Satb2flx/flx cultures, Bic  - 910; Satb2NesCre cultures, NBQX -604, Satb2NesCre cultures, Bic  - 835). Data are presented as mean ± SEM, ***p &lt; 0.001."
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
SATB2: 212712
Satb2: 212712"
Figure legend: "Confocal  images (z-axis-projected stacks) of Lamin B2 stained neuronal nuclei from the CA1 pyramidal cell layer of adult Satb2flx/flx and Satb2flx/flx::CamK2a-Cre (Satb2CamkCre) mice. Scale bars: 10 μm."
NCBI gene IDs:
"Camk: 12322
CamK2a: 12322
Cre: 2777477
Satb2: 212712"
Figure legend: "Analysis of the percentage of infolded nuclei in the hippocampus  of  Satb2flx/flx mice vs Satb2CamkCre mice. In Satb2flx/flx mice, the number of infolded nuclei  was significantly higher in the CA1 area compared to the CA3  area (p = 0.000001) and DG  (p = 1.8 E-12). In Satb2CamkCre mice, the number of infolded nuclei in the CA1 area was significantly reduced compared to the corresponding number in littermate controls (p = 1.9 E-09). No significant differences were observed in this number in the CA3 and DG between Satb2CamkCre mice and floxed controls (n = 4 mice, two-way ANOVA, significant interaction, F2,18 = 47.81, p = 0.00001, simple main effects analysis, CA1 area, Satb2flx/flx vs CKO mice p = 1.9 E-09, CA3  area, Satb2flx/flx vs CKO mice  p = 0.909,  DG, Satb2flx/flx vs CKO mice  p = 0.262, adjustment for multiple comparisons: Bonferroni). Number of analyzed nuclei: Satb2flx/flx mice: 469 (CA1), 225 (CA3), 665 (DG); Satb2CamkCre: 508 (CA1), 224 (CA3), 575 (DG). Data are presented as mean ± SEM, ***p &lt; 0.001."
NCBI gene IDs:
"Camk: 12322
Cre: 2777477
Satb2: 212712"
Figure legend: "Analysis of the percentage of infolded nuclei  in the CA1 pyramidal cell layer of  Satb2CamkCre mice  vs. littermate controls after stereotaxic injections of AAV-SATB2-V5  or AAV8-EGFP. Viral delivery of  SATB2  resulted in restoration of the number of infolded nuclei  in the CA1 pyramidal cell  layer of Satb2CamkCre mice  (n = 3-5 mice, one-way ANOVA followed by Hochberg post hoc test, F2,9 = 33.1, Satb2CamkCre::AAV-SATB2 vs. Satb2flx/flx::AAV-EGFP, p = 0.0012; Satb2flx/flx::AAV-EGFP vs. Satb2CamkCre::AAV-EGFP, p = 0.00008, Satb2flx/flx::AAV-EGFP vs. Satb2CamkCre::AAV-SATB2, p = 0.072). Number of analyzed  nuclei: 388 (Satb2flx/flx::AAV-EGFP), 560 (Satb2CamkCre::AAV-EGFP), 546 (Satb2CamkCre::AAV-SATB2). Data are presented as mean ± SEM, ***p &lt; 0.001 compared to  Satb2flx/flx::AAV-EGFP, ##p &lt; 0.01, compared to Satb2CamkCre::AAV-EGFP."
NCBI gene IDs:
"V5: 
EGFP: 
Camk: 12322
Cre: 2777477
Satb2: 212712
SATB2: 212712"
Figure legend: "Nuclear  infolding triggered by SATB2  overexpression requires LEMD2. Analysis of the percentage of infolded nuclei  in DIV10 hippocampal neurons  upon Satb2  overexpression and Lemd2  knockdown. AAV-SATB2-transduced neurons (both non-transfected and scrambled siRNA-transfected, Scr) exhibited higher percentage of infolded nuclei  compared to AAV-EGFP-transduced neurons. In contrast, LEMD2-depleted AAV-SATB2-transduced cultures showed similar percentage of infolded nuclei  to  AAV-EGFP-transduced neurons (n = 3-4 independent primary cultures; one-way ANOVA F3,11 = 30.982, p = 0.000011; Hochberg post hoc test, AAV-EGFP vs. AAV-SATB2 p = 0.0001, AAV-EGFP vs. AAV-SATB2 + Scr p = 0.0001,  AAV-EGFP vs.  AAV-SATB2 + siLemd2 p= 0.9999, AAV-SATB2 + Scr vs. AAV-SATB2  + siLemd2 p = 0.0001). Number of nuclei  analyzed: 460 (AAV-EGFP), 466 (AAV-SATB2), 537 (AAV-SATB2 + Scr), 597 (AAV-SATB2+siLem2). Data are presented as mean ± SEM, ***p &lt; 0.001 compared to AAV-EGFP; ###p &lt; 0.001 compared to AAV-SATB2 + Scr."
NCBI gene IDs:
"EGFP: 
Lemd2: 224640
Lem2: 224640
LEMD2: 224640
Satb2: 212712
SATB2: 212712"
Figure legend: "Activity-induced formation of nuclear  infoldings is impaired in LEMD2-depleted cortical neurons. Bic-triggered AP bursting for 1 h caused a significant increase in the percentage of infolded nuclei  in  cortical neurons transduced with AAV-scrambled but not with AAV-shLemd2  virus (n = 5 independent primary cultures, two-way ANOVA, F1,16 = 8.35, significant interaction p = 0.0107, simple main effects analysis: AAV-scrambled-transduced cultures, Bic-treated vs untreated p = 0.0049, AAV-shLemd2-transduced cultures, Bic-treated vs untreated p &gt; 0.99, adjustment for multiple comparisons: Bonferroni). Data are presented as mean ± SEM, **p &lt; 0.01 compared to NBQX-treated cultures."
NCBI gene IDs:
"Lemd2: 224640
LEMD2: 224640"
Figure legend: "LEMD2  is required for the nuclear envelope infoldings of CA1 pyramidal neurons in vivo. Top panel: representative confocal  images (z-axis-projected stack) of Lamin B2/DAPI-stained coronal brain   sections from wild-type mice after stereotaxic injection of AAV-shRNA viruses (AAV-shLemd2-mCherry or  AAV-scrambled-mCherry) into the dorsal hippocampus. Merged,  colocalization of the three signals. Scale bars: 50 μm. Middle panel: higher magnification images of AAV-shLemd2-transduced CA1 pyramidal neurons. Arrows show examples of mCherry-positive nuclei that are devoid of nuclear infoldings, whereas stars denote non-transduced infolded nuclei. Lower panel: higher magnification images of AAV-scrambled siRNA-transduced  CA1 pyramidal neurons. Arrows show examples of infolded, mCherry-positive nuclei; stars denote infolded, non-transduced neurons. Scale bars (middle and lower panels): 10 μm."
NCBI gene IDs:
"mCherry: 
LEMD2: 224640
Lemd2: 224640"
Figure legend: "Analysis of the percentage of infolded nuclei  in DIV10 hippocampal neurons upon SATB2 overexpression and  Vps4a/ Vps4b knockdown. AAV-SATB2-transduced neurons (non-transfected or scrambled siRNA (Scr)-transfected) exhibited higher percentage of infolded nuclei  compared to AAV-EGFP-transduced neurons. In contrast, VPS4-depleted  AAV-SATB2-transduced cultures showed similar number of infolded nuclei to AAV-EGFP-transduced neurons (n = 4 independent primary cultures, one-way ANOVA followed by Tuckey post hoc test, F3,11 =15.866, p = 0.00026, AAV-EGFP vs. AAV-SATB2 p = 0.0104, AAV-EGFP vs. AAV-SATB2 + Scr p = 0.0028, AAV-EGFP vs. AAV-SATB2 + siVps4 p = 0.605, AAV-SATB2 + Scr vs. AAV-SATB2 + siVps4 p = 0.0007). Number of  nuclei  analyzed: 594 (AAV-EGFP), 657 (AAV-SATB2), 612 (AAV-SATB2 + Scr), 428 (AAV-SATB2 + siVps4). Data are presented as mean ± SEM, **p &lt; 0.01 compared with AAV-EGFP; ###p &lt; 0.001 compared with AAV-SATB2 + Scr."
NCBI gene IDs:
"EGFP: 
SATB2: 212712
Vps4a: 116733
Vps4: 116733///20479
Vps4b: 20479
VPS4: 20479///116733
Vps4: 20479///116733"
Figure legend: "Activity-induced formation of nuclear  infoldings is impaired in VPS4-depleted cortical neurons. Upon AP bursting, the percentage of infolded nuclei was increased in non-transfected cultures and in cultures transfected with scramble siRNA (Scr) but not with siVps4 (n = 3-4 independent primary cultures, one-way ANOVA followed by Tuckey post hoc test, F3,8 = 90.525, p = 1.63E-06,  Bic-treated vs NBQX-treated p = 0.000012, Scr Bic-treated vs NBQX-treated p = 0.000052, siVps4 Bic-treated vs NBQX-treated p = 0.396, siVps4 Bic-treated vs Scr Bic-treated p = 0.000015). Number of analyzed  nuclei: 474 (NBQX), 456 (Bic), 409 (Bic + Scr), 383 (Bic + siVps4). Data are presented as mean ± SEM, ***p &lt; 0.001 compared to NBQX-treated, ###p &lt; 0.001 compared to Scr Bic-treated."
NCBI gene IDs:
"VPS4: 116733///20479
Vps4: 116733///20479
Vps4: 20479///116733"
Figure legend: "Representative confocal  images (Z-axis projection) of CA1 pyramidal neurons of: (top panel) Satb2 floxed mice  (n = 3) maintained in their home cages (HC), (middle panel) Satb2 floxed mice  (n = 4) subjected to NEE  for 1.5 h, (bottom panel) Satb2CamkCre mice  (n = 5) exposed to NE for 1.5 h. Brain  sections were stained for cFos and Lamin B2. Scale bars: 10 μm. Higher magnification views of boxed areas in A (middle panel). Arrows show examples of infolded, cFos-positive nuclei  of the CA1 field of Satb2 floxed mice  subjected to  NEE. Scale bars: 5 μm."
NCBI gene IDs:
"Camk: 12322
Cre: 2777477
Satb2: 212712"
Figure legend: "(f,g) Northern blot analyses of TbMCU RNAi of BSF (f) and PCF (g) trypanosomes grown in the absence (0) and presence (2-8; 2-10) of tetracycline (top). Tubulin is shown as a loading control (bottom). Markers are shown on the right. The transcript of TbMCU, including the 5′- and 3′-UTR was ~1.5 kb in length."
NCBI gene IDs:
"TbMCU: 
Tubulin: "
Figure legend: "(h,i) Western blot analyses of TbMCU RNAi of BSF (h) and PCF (i) trypanosomes grown in the absence (0) or presence (2-8; 2-10) of tetracycline. Total lysates (30 μg) were subjected to 10% SDS-polyacrylamide gel electrophoresis before transfer to a nitrocellulose membrane and then stained with antibodies against TbMCU (top). One band of ~30 kDa was detected in T. brucei homogenates. Membranes were stripped and re-incubated with antibody against the voltage-dependent anion channel (TbVDAC) as a loading control (bottom)."
NCBI gene IDs:
"TbMCU: "