EMBO/SourceData huggingface dataset:
Figure legend: "C qRT-PCR detection of Il6, IL-1β and Tnfα mRNA levels in Ccdc50+/+ and Ccdc50-/- BMDMs primed with LPS for 3 h and then induced with ATP and Nigericin (A),  L.m and  S.t (B), or MSU  and  SiO2 (C) for indicated time points (n = 3 biological replicates). D-F Ccdc50+/+ and  Ccdc50-/- BMDCs treated as BMDMs  were collected and subjected to qRT-PCR analysis (n = 3 biological replicates). Data information: mRNA data are normalized to GAPDH  and relative to untreated wild-type cells; Data are representative of three biological replicates and are shown as the mean with SEM *P &lt;0.05, **P &lt; 0.01, ***P &lt; 0.001; two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ccdc50: 67501
GAPDH: 14433
IL-1β: 16176
Il6: 16193
Tnfα: 21926"
Figure legend: "G ELISA  analysis of the protein level of secreted  IL-1β in supernatants of Ccdc50+/+ and Ccdc50-/- BMDMs  (top panel) and BMDCs  (bottom panel) treated as in (A-C) (n = 3 biological replicates). Data information: Data are representative of three biological replicates and are shown as the mean with SEM *P &lt;0.05, **P &lt; 0.01, ***P &lt; 0.001; two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ccdc50: 67501"
Figure legend: "H,I Ccdc50+/+ and  Ccdc50-/- BMDMs (H) and  BMDCs (I) were treated as in (A-C). The cells were lysed for western blotting analysis of  pro-caspase-1  and the supernatant was collected for  immunoblots of cleaved-caspase-1. The expression levels of cleaved-caspase-1 were quantitated using ImageJ software."
NCBI gene IDs:
"Ccdc50: 67501"
Figure legend: "F Immunoblot analysis of lysates from HEK293  cells cotransfected with Flag- AIM2, -NLRP3, -ASC and  HA-CCDC50 or empty control vector. The expression  levels of Flag-tagged proteins and Actin were quantitated using ImageJ software. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"Flag: 
HA: 
AIM2: 9447
CCDC50: 152137
NLRP3: 114548
ASC: 29108"
Figure legend: "G  Immunoblot analysis of  HEK293 cells transfected with FLAG-NLRP3 and an increasing amount of  HA-CCDC50 plasmids. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"FLAG: 
HA: 
CCDC50: 152137
NLRP3: 114548"
Figure legend: "H Immunoblot  analysis of lysates from CCDC50-KO and CCDC50-WT  THP-1  cells induced with  LPS  plus  ATP and then treated with cycloheximide (CHX) for indicated  time points. The expression  levels of NLRP3 and  Actin were quantitated using ImageJ software. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "I  HEK293  cells were co-transfected with Flag-NLRP3 and HA-CCDC50 or empty control vector for 24 h and then treated with MG132, NH4Cl, 3-MA, CQ or DMSO  for another 6 h. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"Flag: 
HA: 
CCDC50: 152137
NLRP3: 114548"
Figure legend: "J Immunoblot analysis of NLRP3 in CCDC50-WT and CCDC50-KO THP-1 cells stimulated with LPS  in combination with  ATP. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "K Western blotting analysis of ATG5 wild-type or knockout  HEK293T cells co-transfected with Flag-NLRP3 and  HA-CCDC50  or empty control vector for 24 h. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"Flag: 
HA: 
ATG5: 9474
CCDC50: 152137
NLRP3: 114548"
Figure legend: "L Immunoblot  analyses of NLRP3 in HEK293  cells in the presence of CCDC50  or its mutant  CCDC50 with the deletion of MIUs. Data information: actin was used as a loading control. All the experiments were repeated at least once."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "A Denature-IP (with anti-Flag) and immunoblot analysis of polyubiquitinated NLRP3 in HEK293  cells transfected with Flag-NLRP3, HA-Ub (ubiquitin) as well as HA- CCDC50 or empty vector for 24 h."
NCBI gene IDs:
"Flag: 
HA: 
CCDC50: 152137
NLRP3: 114548"
Figure legend: "F The colocalization  analysis of NLRP3 and LC3B  in CCDC50-WT and CCDC50- KO  HeLa cells stimulated with LPS plus ATP; scale bars, 5 μM. Data information: The nuclei were  stained with DAPI; data are representative of three individual experiments."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "G The  oligomerization analysis  of  NLRP3 in CCDC50 wild-type and knockout THP-1  cells stimulated with  LPS as well as ATP. LPS-primed (1 μg/ml, 3 h)  THP-1 cells were stimulated with  ATP (5 mM) for another 30 min and then were collected and lysed. The cell lysates were centrifuged and separated into soluble  and insoluble fractions. The soluble fraction was analyzed by SDS-PAGE. The insoluble fraction was analyzed by  native-PAGE."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "H The  oligomerization analysis  of NLRP3 in HEK293  cells transfected with plasmids expressing Flag-NLRP3, HA-CCDC50  or empty vector for 24 h, and then treated with  DMSO or CQ for 6 h. After treatment, the cells were collected and lysed. The cell lysates were centrifuged and separated into soluble and insoluble fractions. The soluble fraction was analyzed by SDS-PAGE. The  insoluble fraction was analyzed by  native-PAGE."
NCBI gene IDs:
"Flag: 
HA: 
CCDC50: 152137
NLRP3: 114548"
Figure legend: "J Confocal microscopy analysis of colocalization  between NLRP3  and ASC  in CCDC50-WT and CCDC50-KO HeLa cells stimulated with LPS plus  ATP; scale bars, 5 μM. Data information: The  nuclei  were stained  with DAPI; data are representative of three individual experiments."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "K Confocal microscopy  analysis of ASC speck formation in CCDC50-WT and CCDC50-KO HeLa cells stimulated with LPS plus ATP; scale bars, 5 μM. Data information: The nuclei  were stained with DAPI; data are representative of three individual experiments."
NCBI gene IDs:
"CCDC50: 152137"
Figure legend: "(E) CSF1R, MRC1, TLR2 and FOS relative expression measured by qRT-PCR for LGCs (upper), FBGCs (middle) and osteoclasts (lower), following the cell membrane fusion regulator DC-STAMP knockdown. si-Ctrl, scrambled siRNA; si-DC-STAMP, DC-STAMP siRNA; n=7 donors."
NCBI gene IDs:
"CSF1R: 1436
DC-STAMP: 81501
FOS: 2353
MRC1: 4360
TLR2: 7097"
Figure legend: "(A) Real-time quantitative PCR (RT-qPCR) of transcripts changing differentially between compartments using the compartmentalized cultures after 48 hours PTX-treatment. n=8 independent biological replicates (except for Plk2 (n=7), where one measurement couldn't be performed due to insufficient cDNA); PTX-effect was assessed by three-way ANOVA followed by Tukey's post-hoc multiple comparison test; *p &lt; 0.05; **p &lt; 0.01."
NCBI gene IDs:
"Plk2: 83722"
Figure legend: "(B) Representative images of single-molecule FISH (smFISH) in either control or 48 h PTX-treated rat hippocampal neurons (DIV20) using probes specific for Add2, Dnajc6 and Sort1 (green). MAP2 immunostaining (red) was used to visualize neuronal somata and dendrites. Inserts at higher magnification illustrate PTX-dependent changes in dendritic RNA puncta. Scale bar = 10 μm. (C) Quantification of B. (n=3-4 independet biological replicates with 8-10 cells averaged per condition and replicate; two-sample Student's t-test; *p &lt; 0.05; **p &lt; 0.01). B"
NCBI gene IDs:
"Add2: 24171
Dnajc6: 313409
Sort1: 83576"
Figure legend: "A, B, Western blotting analysis of proteins co-precipitated with anti-FLAG antibody from testis extracts of E15.5 wild-type embryos and transgenic embryos expressing FLAG-tagged NANOS2 with or without RNase (A) or from extracts of HeLa cells transfected with FLAG-tagged DND1 and with or without HA-tagged NANOS2 (B)."
NCBI gene IDs:
"DND1: 373863
NANOS2: 378430
NANOS2: 339345"
Figure legend: "Precipitates were analyzed with the indicated antibodies. C, GST pull-down assay using E. coli extracts expressing GST, GST-fused NANOS2, or NANOS3 mixed with MBP-tagged LacZ or DND1. Precipitates were analyzed by Coomassie Brilliant Blue (CBB) staining or western blotting with an anti-MBP antibody. An arrowhead indicates MBP-DND1 co-precipitated with GST-tagged NANOS2 or NANOS3."
NCBI gene IDs:
"LacZ: 
DND1: 373863
NANOS2: 339345
NANOS3: 342977"
Figure legend: "D, E, Western blotting analyses of proteins co-precipitated with anti-FLAG antibody from extracts of HeLa cells transfected with FLAG-tagged NANOS2, NANOS2 (ΔN10), NANOS2 (ΔC10), NANOS2 (C61A, C96A) or NANOS3, and HA-tagged DND1 (D)"
NCBI gene IDs:
"DND1: 373863
NANOS2: 339345
NANOS3: 342977"
Figure legend: "D, E, Western blotting analyses of proteins co-precipitated with anti-FLAG antibody from extracts of HeLa cells transfected with FLAG-tagged NANOS2, NANOS2 (ΔN10), NANOS2 (ΔC10), NANOS2 (C61A, C96A) or NANOS3, and HA-tagged DND1 (D) or with FLAG-tagged NANOS2, NANOS2 (C61A), NANOS2 (C96A) or NANOS2 (C61A, C96A), and HA-tagged DND1 (E)."
NCBI gene IDs:
"DND1: 373863
NANOS2: 339345"
Figure legend: "Precipitates were analyzed with the indicated antibodies. F, GST pull-down assay using E. coli extracts expressing GST-fused DND1 mixed with MBP-tagged LacZ, NANOS2, NANOS2 (C61A, C96A) or NANOS2 (60-114). Arrowheads indicate MBP-tagged NANOS2 (lane 2) or NANOS2 (60-114) (lane 4)."
NCBI gene IDs:
"LacZ: 
DND1: 373863
NANOS2: 339345"
Figure legend: "Western blotting analyses of proteins co-precipitated with anti-FLAG antibody from extracts of HeLa cells transfected with HA-tagged NANOS1, NANOS2 or NANOS3, and FLAG-tagged DND1 (A)"
NCBI gene IDs:
"DND1: 373863
NANOS1: 340719
NANOS2: 339345
NANOS3: 342977"
Figure legend: "Western blotting analyses of proteins co-precipitated with anti-FLAG antibody from extracts of HeLa cells transfected with HA-tagged NANOS1, NANOS2 or NANOS3, and FLAG-tagged DND1 (A) or with FLAG-tagged PUMILIO1 or PUMILIO2, and HA-tagged NANOS1, NANOS2 or NANOS3 with or without MYC-tagged DND1 (B)."
NCBI gene IDs:
"DND1: 373863
NANOS1: 340719
NANOS2: 339345
NANOS3: 342977
PUMILIO1: 9698
PUMILIO2: 23369"
Figure legend: "C, Characterization of antibodies against PUMILIO1 and PUMILIO2. Western blotting analyses of Flag-tagged PUMILIO1 and PUMILIO2 in HeLa cells transfected with FLAG-tagged PUMILIO1 or PUMILIO2 using antibodies against FLAG, PUMILIO1 or PUMILIO2. Note that the antibodies against PUMILIO1 specifically recognized FLAG-tagged PUMILIO1 while the anti-PUMILIO2 antibody could detect both Flag-tagged PUMILIO1 and PUMILIO2 but less actively."
NCBI gene IDs:
"PUMILIO1: 9698
PUMILIO2: 23369"
Figure legend: "D, FLAG-tagged NANOS2 was immunoprecipitated with an anti-FLAG antibody from E15.5 testicular extracts from transgenic mice expressing FLAG-tagged NANOS2. Precipitates were analyzed by western blotting with the indicated antibodies. Note that co-precipitation of both PUMILIO1 and PUMILIO2 were not detected even though both CNOT1 and DND1 were clearly co-precipitated."
NCBI gene IDs:
"NANOS2: 378430"
Figure legend: "Figure 3 | DND1 co-localizes with NANOS2 in P-bodies. A−C, Sections of male gonads from E15.5embryos were immunostained with antibodies against DND1 (green) and DCP1a (red). D−F, Squash preparation of a male gonocyte from E16.5embryoimmunostained with antibodies against DND1 (red) and DCP1a (green). Arrowheads indicate co-localization of DND1 and DCP1a. G−I, Sections of male gonads from E15.5embryos were immunostained with antibodies against DND1 (red) and NANOS2 (green). Arrowheads indicate co-localization of DND1 and NANOS2. J−M, NIH3T3 cells transfected with HA-tagged Dnd1 and FLAG-tagged Nanos2 were then immunostained with antibodies against DND1 (J) (magenta), NANOS2 (K) (red), and DCP1a (L) (green). N−Y, Biomolecular fluorescence complementation assay. NIH3T3 cells transfected with VENUS-C-fused Dnd1 and VENUS-N-fused Nanos2 (N−U) or Nanos2 (C61A, C96A) (V-γ) were immunostained with antibodies against DND1 (N, S, V), NANOS2 (O, W, α) or DDX6 (R, Z). Then, the signals of VENUS fusion protein were visualized (P, T, X, β). Arrowheads in Z indicate P-bodies. DNA was labeled with DAPI (blue). Scale bars: 50 m in A for A−C; 10 m in D for D-F, G for G−I, J for J−M, N for N−γ. Insets in A-C and J-γ show an enlarged vision of each picture to better depict localization of each protein. See also Fig. EV2T-Y."
NCBI gene IDs:
"Dnd1: 213236
Nanos2: 378430"
Figure legend: "A, Western blotting analyses of proteins in testes from E13.5 to E16.5 embryos of Dnd1flox/flox or Dnd1flox/flox_Tg(Oct4PE-CreERT2) each administered with tamoxifen at E13.5 and E15.5 embryos of Nanos2+/- or Nanos2-/- with the indicated antibodies."
NCBI gene IDs:
"CreERT2: 
Oct4PE: 
Dnd1: 213236
Nanos2: 378430"
Figure legend: "B-P, Sections of testes from Dnd1flox/flox (B, E, H, K, N), Dnd1flox/flox_Tg(Oct4PE-CreERT2) (C, F, I, L, O) or Nanos2-/- (D, G, J, M, P) embryos were prepared at E16.5 and then immunostained with antibodies against pH3 (B-D), STRA8 (E-G), SYCP3 (H-J), activated-Caspase3 (K-M) or LAMININ (N-P) (green). Germ cells were immunostained with TRA98 (B-D) or DAZL (N-P) (red), and DNA was labeled with DAPI (blue). Tamoxifen was administered at E13.5. Scale bars: 50 m in B for B−D and K-P, 50 m in E for E−J. See also Fig. EV3."
NCBI gene IDs:
"Dnd1: 213236
Nanos2: 378430"
Figure legend: "A-L, Sections of testes from Dnd1flox/flox (A, B, C, G, H, I, M, N, O) and Dnd1flox/flox_Tg(Oct4PE-CreERT2) (D, E, F, J, K, L, P, Q, R) embryos were prepared at E16.5 and then immunostained with antibodies against NANOS2 (A, D) (green), DCP1a (G, J) (green), DDX6 (M, P) (green) and TRA98 (B, E, H, K, N, Q). DNA was labeled with DAPI (blue). Tamoxifen was administered at E13.5. Scale bars: 50 m in A for A−F; 50 m in G for G-L; 50 m in M for M-R. Insets show an enlarged vision of each picture to better depict localization of NANOS2, DCP1a and DDX6."
NCBI gene IDs:
"Oct4PE: 
CreERT2: 
Dnd1: 213236"
Figure legend: "S-V, Immunoprecipitation with an anti-FLAG antibody from E15.5 male gonadal extracts of wild-type and the transgenic mouse line expressing FLAG-tagged DND1 (S, T), or with an anti-NANOS2 antibody from E15.5 male gonadal extracts of Dnd1flox/flox mice with or without Oct4PE-CreERT2 (U, V). Precipitates were analyzed by western blotting (S, U) or by RT-qPCR (T, V). The RT-qPCR data are shown as average relative mRNA levels ± SEs (n=3)."
NCBI gene IDs:
"Oct4PE: 
CreERT2: 
DND1: 213236
Dnd1: 213236"
Figure legend: "W, X, Western blotting analyses of proteins in NANOS2-depleted (W) or DND1-depleted (X) testis extracts from E15.5 embryos. Proteins were analyzed with the indicated antibodies."
NCBI gene IDs:
"DND1: 213236
NANOS2: 378430"
Figure legend: "C Comparison of meiotic chromosome segregation in cells lacking H3K9me3 depending on Swi6's affinity towards H3K9me2. Exemplary IF microscopy images of the indicated fission yeast strains are shown. Homothallic strains were incubated on SPAS plates. DNA and tubulin were stained with DAPI and anti-TAT-1, respectively. Missegregating chromosome is indicated with a yellow arrow; D Quantification of meiotic chromosome segregation phenotypes. The data for clr4+ and clr4F449Y is the same as in Fig 3B. P values were calculated using Fisher's exact test. Number of total cells counted is indicated in each bar."
NCBI gene IDs:
"clr4: 2540825
Swi6: 2541633"
Figure legend: "E Comparison of co-segregation of centromere I (CenI) during MI in cells lacking H3K9me3 depending on Swi6's affinity towards H3K9me2. Exemplary live cell microscopy images of the indicated fission yeast strains during MI and MII are shown. Heterothallic strains were crossed and dyads as well as tetrads were subjected to the analysis. Heterozygous CenI-GFP was used to follow the segregation pattern during meiosis. The GFP-signal is additionally displayed in greyscale for better visibility F Quantification of CenI-GFP segregation during meiosis. The data for clr4+ and clr4F449Y is the same as in Fig 3D. P values were calculated using Fisher's exact test. Number of total cells counted is indicated in each bar."
NCBI gene IDs:
"clr4: 2540825
Swi6: 2541633"
Figure legend: "G Tetrad dissection to asses spore viability in clr4F449Y swi6Chp1-like-CD cells. Exemplary image (left) and quantification of the spore viability (right) are shown. Meiosis was induced in homothallic cells and the resulting spores were dissected. The data for clr4+ and clr4F449Y is the same as in Fig 3F. P values were calculated using Fisher's exact test. Number of dissected tetrads is indicated in each bar."
NCBI gene IDs:
"clr4: 2540825
swi6: 2541633"
Figure legend: "B. Violin plots depicting the distribution of the quantified average cellular expression of Prok2, ProkR2, Vip, Vipr2, Avp, Avpr1a, Grp and Grpr in each sub-cluster in CT7.5 (left) and CT15.5 (right) datasets."
NCBI gene IDs:
"Avp: 11998
Avpr1a: 54140
Grp: 225642
Grpr: 14829
Prok2: 50501
ProkR2: 246313
Vip: 22353
Vipr2: 22355"
Figure legend: " C. The period of Cry1-luc bioluminescence rhythms in SCN slices transfected with either pProk2.Cre.T2A.mCherry or pProkR2.Cre.T2A.Venus does not differ between treatments (p=0.71, n= 10, 9 SCN slices per group, respectively). D. Similarly, the amplitude of Cry1-luc bioluminescence rhythms from Prok2+ SCN cells and ProkR2+ SCN cells, does not differ between treatments (p=0.67). "
NCBI gene IDs:
"luc: 
mCherry: 
Venus: 
Cre: 2777477
Cry1: 12952
Prok2: 50501
ProkR2: 246313"
Figure legend: " H. Left: confocal images of representative SCN slices dual-transfected with pProk2.Cre.T2A.mCherry and Syn.GCaMP6f (top), or pProkR2.Cre.T2A.Venus and Syn.jRCaMP1a (below) (scale bar=500µm). Right, above: representative detrended plots of signal from GCaMP6f (green) traces and pCry1-DIO-Luc, emitted from Prok2+ SCN cells (red). Right, below: representative detrended plots of jRCaMP1a (purple) and pCry1-DIO-Luc emitted from ProkR2+ cells (light green). Orientation bars depict the horizontal axis of the optic chiasm and the vertical axis of the third ventricle for each SCN. "
NCBI gene IDs:
"GCaMP6f: 
jRCaMP1a: 
Luc: 
mCherry: 
Venus: 
Cre: 2777477
Cry1: 12952
Prok2: 50501
ProkR2: 246313
Syn: 20964"
Figure legend: " B. Comparison of the Per2::Luciferase rhythm amplitude of the cycle preceding and the first and second cycles succeeding vehicle (applied CT23-20 dark grey n=12 SCN slices, CT-20-23 light grey n=6 SCN slices) or Prok2RA (applied CT23-20 purple n=13 SCN slices, CT20-23 pink n=11 SCN slices) treatment (interaction, p&lt;0.0001). A significant decline in amplitude occurred when Prok2RA signalling was blocked between CT20-23 (****p&lt;0.0001). "
NCBI gene IDs:
"Luciferase: 
Per2: 18627"
Figure legend: "  H. Period lengthening occurred at the same rate when Prok2.Cre or ProkR2.Cre was the first AAV to be applied (p=0.1; Prok2.Cre - baseline vs. 1 AAV, *p=0.01; baseline vs. both AAVs, *p=0.04; ProkR2.Cre - baseline vs. 1 AAV, *p=0.03; baseline vs. both AAVs, **p=0.01. "
NCBI gene IDs:
"Cre: 2777477
Prok2: 50501
ProkR2: 246313"
Figure legend: " A. Representative Per2::Luciferase traces of Cry1,2-/- SCN slices at baseline (black, arrhythmic), after Cry1 complementation in Prok2+ cells (red) and further complementation in ProkR2+ cells (yellow, rhythmic) (cohort n=8). B. as in A but order of complementation reversed (green =Cry1 complementation in ProkR2+ cells) (cohort n=8). "
NCBI gene IDs:
"Luciferase: 
Cry1: 12952
Per2: 18627"
Figure legend: "AR-dependent luciferase assays were performed on LNCaP  cells transfected with siTRIM33 or negative control (NC) (A), increasing amounts of TRIM33  plasmid (B)  Western blot analysis shows the protein level of TRIM33  in LNCaP  cells after TRIM33  knockdown (A) or overexpression (B). Data information: , data are presented as mean ± SD (n = 3 independent experiments). *P &lt; 0.05; **P &lt; 0.01; ns=not significant (two-way ANOVA)."
NCBI gene IDs:
"TRIM33: 51592"
Figure legend: "A-D, AR  transcript levels were measured by  RT-qPCR on LNCaP  cells (A and C) or VCaP  cells (B and D) transfected with siTRIM33  or the TRIM33  expression construct and treated with EtOH  or DHT. Protein lysates were harvested at 72 h after transfection and subjected to western blot  analysis with the indicated antibodies. Data information: In (A-D, data are presented as mean ± SD (n = 3 independent experiments). * P &lt; 0.05, **P &lt; 0.01, ns=not significant (two-way ANOVA)."
NCBI gene IDs:
"AR: 367
TRIM33: 51592"
Figure legend: "E and F, LNCaP  cells transfected with siTRIM33 or NC were treated with cycloheximide  (CHX). Protein lysates were harvested at the indicated time points for  western blot analysis. AR  protein levels were quantified and normalized to β-actin and then plotted relative to 0 h. Data information: In F, data are presented as mean ± SD (n = 3 independent experiments). * P &lt; 0.05, **P &lt; 0.01, ns=not significant (two-way ANOVA)."
NCBI gene IDs:
"TRIM33: 51592"
Figure legend: "G, LNCaP  cells transfected with siTRIM33  or NC were treated with DMSO  or MG132  for 8 h. Protein lysates were extracted and subjected to western blot analysis with the indicated antibodies."
NCBI gene IDs:
"TRIM33: 51592"
Figure legend: "HA-Ub   was expressed in LNCaP  cells co-transfected with siTRIM33 (H) The cells were treated with MG132  for 4 h before harvesting. Endogenous AR  was immunoprecipitated  from cell lysates, and interacting  proteins were detected by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"HA: 
TRIM33: 51592
Ub: 7311///7316///7314///6233"
Figure legend: "I, HA-Ub  was expressed in LNCaP  cells co-transfected with the TRIM33  expression construct (I). The cells were treated with MG132  for 4 h before harvesting. Endogenous AR  was immunoprecipitated  from cell lysates, and interacting  proteins were detected by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"HA: 
TRIM33: 51592
Ub: 7311///7316///7314///6233"
Figure legend: "LNCaP  cells transiently expressing myc-tagged Skp2  with Flag-TRIM33  were harvested and subject to immunoblot  analysis with the indicated antibodies."
NCBI gene IDs:
"Flag: 
myc: 
Skp2: 6502
TRIM33: 51592"
Figure legend: "K, LNCaP  cells transiently expressing myc-tagged SPOP  with Flag-TRIM33  were harvested and subject to immunoblot analysis with the indicated antibodies."
NCBI gene IDs:
"Flag: 
myc: 
SPOP: 8405
TRIM33: 51592"
Figure legend: "L, shTRIM33- or shCtrl-LNCaP  cell lines transfected with NC or siSkp2 were harvested 72 h after transfection and subjected to immunoblot  analysis with the indicated antibodies."
NCBI gene IDs:
"Skp2: 6502
TRIM33: 51592"
Figure legend: "M, HEK293T  cells were co-transfected with flag-TRIM33   and various myc-tagged AR  constructs.  AR-FL and AR truncated mutants were immunoprecipitated  with an anti-myc  antibody and then subjected to western blot analysis with the indicated antibodies."
NCBI gene IDs:
"myc: 
AR: 367"
Figure legend: "N, HEK293T   cells were co-transfected with myc-AR-N and various flag-tagged TRIM33  constructs. AR-N was immunoprecipitated with an anti-myc  antibody and then subjected to western blot  analysis with the indicated antibodies."
NCBI gene IDs:
"flag: 
myc: 
AR: 367
TRIM33: 51592"
Figure legend: "O, LNCaP  cells were transfected with various flag-tagged TRIM33  constructs. AR-associated proteins were immunoprecipitated  with an anti-AR  antibody and then subjected to western blot  analysis with the indicated antibodies."
NCBI gene IDs:
"flag: 
TRIM33: 51592"
Figure legend: "P, LNCaP  cells were co-transfected with myc-Skp2  and various flag-TRIM33  constructs. AR  and other proteins were detected by western blot  analysis with the indicated antibodies."
NCBI gene IDs:
"flag: 
myc: 
Skp2: 6502
TRIM33: 51592"
Figure legend: "Q, AR pull-down was performed on protein extracts from MG132-treated HEK293T  cells overexpressing myc-AR, HA-Ub, myc-Skp2, and various flag-tagged TRIM33  constructs. AR ubiquitination  and specific protein levels were detected by  western blot  analysis with the indicated antibodies."
NCBI gene IDs:
"flag: 
HA: 
myc: 
AR: 367
Skp2: 6502
TRIM33: 51592
Ub: 7311///7316///7314///6233"