EMBO/SourceData huggingface dataset:
Figure legend: "A. Left panel: Injection and analysis protocol. Right panel: muscle strength analysis demonstrating that hEN1 injection at 1 month prevents muscular strength decrease observed in 3-month-old En1-Het mice. One-way ANOVA followed by two-sided t-test. (*p&lt;0.05, ****p&lt;0.0001). N=6-10. Data information: This experiment was performed once. Values are mean ± SD."
NCBI gene IDs:
"En1: 13798"
Figure legend: "B. Left panel: Increased p62/SQTSM1 staining  in 3-month-old ChAT+ MNs  from control En1-Het is abolished by hEN1 injection at 1 month. Right panel: quantification of p62/SQTSM1 staining  in γMNs  and αMNs  of control and hEN1-injected 3-month-old En1-het mice. One-way ANOVA followed by two-sided t-test. (**p&lt;0.01, ****p&lt;0.0001). Between XXX neurons  in YY mice  were analyzed in this experiment. Data information: This experiment was performed once. Values are mean ± SD. Between 111 and 303 neurons  and 4-5 mice  were analyzed for each condition."
NCBI gene IDs:
"En1: 13798"
Figure legend: "(A) HeLa cells were treated with control or indicated siRNA for 72 hours and SL biosynthesis measured by [3H] - sphingosine pulse-chase assay. GSL levels are expressed as fold changes with respect to control. CERT and FAPP2 knockdowns (blue bars) were used as controls."
NCBI gene IDs:
"CERT: 10087
FAPP2: 84725"
Figure legend: "(D) Control and GRASP55KO cells were processed for Cy3-conjugated Shiga Toxin (ShTxB) and Alexa488-conjugated Cholera Toxin (ChTxB) staining followed by flow cytometry analysis. Mean fluorescence intensity was measured and represented."
NCBI gene IDs:
"GRASP55: 26003"
Figure legend: "(C) Control and GRASP55 KO cells were transfected for 16 hours with GCS-HA or LCS-HA and processed for cryoimmunolabeling with anti-HA antibody (10-nm gold particles) and anti-GM130 antibody (15-nm gold particles) in case of GCS-HA and anti-HA antibody (15-nm gold particles) and anti-GM130 antibody (10-nm gold particles) in case of LCS-HA. Representative images of the distribution of GCS-HA and LCS-HA are shown. Scale bar 200nm. Red asterisk marks C4 cisterna and blue circles indicate GM130 labelling."
NCBI gene IDs:
"GRASP55: 26003"
Figure legend: "(A-C) Control and GRASP55 KO (#2) cells were transfected for 16 hours with the indicated HA-tagged enzymes and processed for cryo-immunolabeling with anti-HA antibody (10-nm gold particles) and anti-GM130 antibody (15-nm gold particles). Representative images of the distribution of HA-tagged enzymes are shown. Red circles indicate GM130 labelling. Arrow heads represent the peri-Golgi vesicles. Scale bar 150 nm. Quantification of the distribution of enzymes in vesicles represented as normalized linear density"
NCBI gene IDs:
"GRASP55: 26003"
Figure legend: "(A Control and GRASP55 KO clones were pre-treated with DMSO or BFA (5μg/ml) for 30 min and SL output monitored by [3H] - sphingosine pulse-chase assay (8 h chase). Total GSL levels were quantified and expressed as fold changes with respect to control (A)."
NCBI gene IDs:
"GRASP55: 26003"
Figure legend: "B) Control and GRASP55 KO clones were pre-treated with DMSO or BFA (5μg/ml) for 30 min and SL output monitored by [3H] - sphingosine pulse-chase assay (8 h chase). The GM/Gb ratio was calculated and represented (B)."
NCBI gene IDs:
"GRASP55: 26003"
Figure legend: "(F) CERT was silenced using siRNAs for 72 hours in control and GRASP55 KO (#2) clone before subjecting to [3H] -sphingosine pulse chase assay. GSLs were quantified and represented as fold change with respect to control."
NCBI gene IDs:
"CERT: 10087
GRASP55: 26003"
Figure legend: " t-SNE maps highlighting the expression of SARS-CoV-2 entry receptors ACE2 Transcript counts are given in a Log2 scale. "
NCBI gene IDs:
"ACE2: 59272"
Figure legend: " O) t-SNE maps highlighting the expression of SARS-CoV-2 entry receptors TMPRSS2 Transcript counts are given in a Log2 scale. "
NCBI gene IDs:
"TMPRSS2: 7113"
Figure legend: " (G)  CHO-K1 Tet-On cells expressing  Z α1-antitrypsin were induced with  doxycycline (0.5μg/ml) for 48 h. Cells were incubated with 10μM GSK716  (or 0.1% v/v DMSO for the control) during the induction. Culture media containing either the experimental compound or DMSO  were changed every 24 h. After the induction, cells were labelled for 10 minutes with 35S Met/Cys and chased at the indicated times. Culture media were collected and cells lysed in 1% v/v NP-40 buffer. Intracellular fractions and culture media from cells expressing Z α1-antitrypsin  were immunoprecipitated either with a mAb against total α1-antitrypsin  (3C11) or with a polymer-specific mAb (2C1). Samples were resolved by 4-12% w/v acrylamide SDS-PAGE  and detected by autoradiography. (H) The graphs show the effect of GSK716 on intracellular and extracellular Z α1-antitrypsin (mean±SEM, n=2) "
NCBI gene IDs:
"Z α1-antitrypsin: 5265"
Figure legend: "Representative calcium signature enriched in P-EMT tumors (comparing ECAD- vs. ECAD+ tumor cells). NES, nominal P-value, and FDR are shown. Statistical analysis performed in GSEA."
NCBI gene IDs:
"ECAD: 12550"
Figure legend: "Representative image of tumor cells transduced with GCaMP6 (Left). Yellow arrows (mesenchymal cells), red arrows (epithelial cells), scale bar = 50μm. GCaMP6 mean fluorescent intensity (MFI) in ECADHIGH or ECADLOW cells cultured in indicated medium (0 Ca2+ or 2 Ca2+) for 1h (Right). Representative of 3 independent murine PDA cell lines run in triplicate. Statistical analysis by Student's unpaired t-test (*, p&lt;0.05; non-significant (NS); ±SD)."
NCBI gene IDs:
"ECAD: 12550"
Figure legend: "Flow cytometry analysis of ECADHIGH or ECADLOW cells loaded with Indo-1 and cultured in indicated medium (0 Ca2+ or 2 Ca2+) for 1h. Representative of 2 independent cell lines (n indicates the number of cells analyzed in each group, ECADHIGH 0 Ca2+ n=4963, ECADLOW 0 Ca2+ n=14, ECADHIGH 2 Ca2+ n=6575, ECADLOW 2 Ca2+ n=29). Representative of 2 independent murine PDA cell lines run in triplicate. Statistical analysis by Student's unpaired t-test (****, p&lt;0.0001; non-significant (NS); ±SD)."
NCBI gene IDs:
"ECAD: 12550"
Figure legend: "Top: Relative mRNA expression of Ecad after treatment with 2.5μM ionomycin or 10ng/ml TGFβ for denoted time. Bottom: Western blot analysis of total E-cadherin and Vimentin protein for denoted time. Experiment was run in duplicate with three different murine PDA cell lines. Statistical analysis by ANOVA (***, p&lt;0.001; non-significant (NS); ±SEM)."
NCBI gene IDs:
"Ecad: 12550"
Figure legend: "MFI of surface E-cadherin in CnB knockout lines after 48h treatment with DMSO, 2.5μM ionomycin, or 10ng/ml TGFβ. Experiment was repeated in two different murine PDA cell lines in triplicate. Statistical analysis by ANOVA (***, p&lt;0.001; ****, p&lt;0.00001; ±SD)."
NCBI gene IDs:
"CnB: 19058"
Figure legend: "MFI of surface E-cadherin of shRNA non-targeting (shNT) (Left) or shCAMK2B (Right) after 48h treatment with DMSO, 2.5μM ionomycin, or 10ng/ml TGFβ. Experiment was repeated in two different murine PDA cell lines. Statistical analysis by ANOVA (***, p&lt;0.001; ****, p&lt;0.00001; ±SD)."
NCBI gene IDs:
"CAMK2B: 12323"
Figure legend: "A-C ARR1/10/12 protein levels were examined by western blot when five-day-old seedlings of 35S:ARR1:MYC, 35S:ARR10:YFP, and 35S:ARR12:YFP were treated with or without 200 mM NaCl for the indicated time points. Actin was used as the internal reference. The relative intensity of band detected by anti-MYC or anti-GFP antibody to that by anti-Actin antibody without treatment was set to 1.0. D-F Quantification analysis of the protein levels of ARR1/10/12 in (A-C). "
NCBI gene IDs:
"MYC: 
YFP: "
Figure legend: "C In vivo Coimmunoprecipitation assay of MPK3/4/6 and ARR1/10/12. MPK3/4/6-YFP and ARR1/10/12-MYC were co-expressed in Arabidopsis protoplast cells. Protein extracts (Input) were immunoprecipitated with GFP Trap magnetic agarose beads. Immunoblots were performed with anti-MYC antibody to detect ARR1/10/12 and with anti-GFP antibody to detect MPK3/4/6."
NCBI gene IDs:
"MYC: 
YFP: 
MPK3: 823706
ARR1: 820940"
Figure legend: "A-C Immunoblot analysis the effects of MPK3/6 on salt stress-induced ARR1/10/12 protein degradation. Seven-day-old seedlings of 35S:ARR1:MYC, 35S:ARR10:YFP, and 35S:ARR12:YFP in Col-0 and MPK3SR backgrounds were treated with or without 200 mM NaCl for indicated time points. Actin was used as the internal reference. The relative intensity of band detected by anti-MYC or anti-GFP antibody to that by anti-Actin antibody without treatment was set to 1.0. D-I Quantification analysis of MPK3/6 effect on salt stress-induced ARR1/10/12 protein degradation in (A-C). "
NCBI gene IDs:
"MPK3: 823706"
Figure legend: "A-C Immunoblot analysis stability of ARR1/10/12 and ARR1T553A/10S383A/12S323A proteins under salt stress. Seven-day-old seedlings of 35S:ARR1:MYC, 35S:ARR10:YFP, 35S:ARR12:YFP, 35S:ARR1T553A:MYC, 35S:ARR10S383A:YFP, and 35S:ARR12S323A:YFP were treated with or without 200 mM NaCl for indicated time points. Actin was used as the internal reference. The relative intensity of band detected by anti-MYC or anti-GFP antibody to that by anti-Actin antibody without treatment was set to 1.0. D-I Quantification analysis of stability of ARR1/10/12 and ARR1T553A/10S383A/12S323A proteins under salt stress in (A-C). "
NCBI gene IDs:
"MYC: 
YFP: 
ARR1: 820940
ARR10: 829322
ARR12: 817056"
Figure legend: "Four-day-old seedlings of 35S:ARR1:MYC, 35S:ARR1T553A:MYC, and 35S:ARR1T553D:MYC were transferred to 1/2 MS medium supplemented with or without 50 mM or 75 mM NaCl. After three days of salt treatment, plant phenotypes (A) were determined."
NCBI gene IDs:
"MYC: 
ARR1: 820940"
Figure legend: "Four-day-old seedlings of 35S:ARR10:YFP, 35S:ARR10S383A:YFP, and 35S:ARR10S383D:YFP were transferred to 1/2 MS medium supplemented with or without 50 mM or 75 mM NaCl. After three days of salt treatment, plant phenotypes (A) were determined."
NCBI gene IDs:
"YFP: 
ARR10: 829322"
Figure legend: "Four-day-old seedlings of 35S:ARR12:YFP, 35S:ARR12S323A:YFP, and 35S:ARR12S323D:YFP were transferred to 1/2 MS medium supplemented with or without 50 mM or 75 mM NaCl. After three days of salt treatment, plant phenotypes were determined."
NCBI gene IDs:
"YFP: 
ARR12: 817056"
Figure legend: "Three-day-old seedlings of Col-0, MPK3SR, MPK3SRarr1/12, and arr1/12 were transferred to 0.5 μM NA-PP1-containing 1/2 MS medium for 2 d to inhibit MPK3TG in MPK3SR and MPK3SRarr1/12 and then the plants were transferred to 0.2 μM NA-PP1-containing 1/2 MS medium supplemented with or without 50 mM or 75 mM NaCl. Pictures were taken and the elongated root length was determined 3 d later"
NCBI gene IDs:
"MPK3: 823706
arr1: 820940"
Figure legend: "A: Morphology  (PAS staining, upper panels) and quantification of glomerular lesions (lower panel) of kidneys from 28-month-old p16 INK-ATTAC mice treated with either vehicle (AP-) or AP20187 (AP+). Original magnification X400. Scale bar = 20 μm. n = 5. B: WT1 immunostaining  (upper panels) and quantification of WT1-positive glomerular cells  (lower panel) in 28-month-old p16 INK-ATTAC mice treated with either vehicle (AP-) or AP20187 (AP+). Original magnification X400. Scale bar = 20 μm. n = 5. C: PAI-1 immunostaining  (upper panels) and quantification of PAI-1-positive glomeruli (lower panel) in 28-month-old  p16 INK-ATTAC mice treated with either vehicle (AP-) or AP20187 (AP+). Original magnification X400. Scale bar = 20 μm. n = 5. Data information: Data are means ± SEM. Statistical analysis: t-student test: AP+ versus AP- mice."
NCBI gene IDs:
"p16 INK: 12578"
Figure legend: "A: PAI-1 immunohistochemistry  (upper panels) and quantification of PAI-1-positive glomeruli (lower panel) in kidneys  of PAI-1flox and PAI-1∆endo mice at 22 months of age. Original magnification X400. Scale bar = 20 μm. n = 4 for PAI-1flox and PAI-1∆endo mice, respectively. B: Morphology  (PAS staining, upper panels) and quantification of glomerular lesions (lower panel) of  kidneys from PAI-1flox and PAI-1∆endo mice at 22 months of age. Original magnification X400. Scale bar = 20 μm. n = 12 for  PAI-1flox and PAI-1∆endo mice, respectively. C: TUNEL assay (upper panels) and quantification of TUNEL-positive glomeruli (lower panel) in kidneys from PAI-1flox and PAI-1∆endo mice at 22 months of age. Original magnification X400. Scale bar = 20 μm. n = 6 for PAI-1flox and PAI-1∆endo mice, respectively. D: WT1 immunostaining  (upper panels) and quantification of WT1-positive  glomerular cells  (lower panel) in kidneys from  PAI-1flox and PAI-1∆endo mice at 22 months of age. Original magnification X400. Scale bar = 20 μm. n = 4 and n = 6 for PAI-1flox and PAI-1∆endo mice, respectively. Data information: Data are means ± SEM. Statistical analysis: t-student test: PAI-1∆endo versus  PAI-1flox mice."
NCBI gene IDs:
"PAI-1: 18787"
Figure legend: "G: p21/griffonia simplicifolia/nephrin coimmunostaining in kidneys (upper panels) from  PAI-1flox and PAI-1∆endo mice at 22 months of age and quantification (lower panel) of p21-positive endothelial cells per  glomeruli. Original magnification X630. Scale bar = 10 μm. n = 4 and n = 6 for PAI-1flox and PAI-1∆endo mice, respectively. H: 53BP1/CD34 coimmunostaining (upper panels) and quantification of glomerular 53BP1 foci in  glomerular endothelial cells  from PAI-1flox and PAI-1∆endo mice  at 22 months of age. Original magnification X630. Scale bar = 10 μm. n=4 and n=6 for PAI-1flox and PAI-1∆endo mice, respectively. Arrows show 53BP1 positive foci. Data information: Data are means ± SEM. Statistical analysis: t-student test: PAI-1∆endo versus  PAI-1flox mice."
NCBI gene IDs:
"PAI-1: 18787"
Figure legend: "C: Relative mRNA expression  of senescence markers (p16  and  p21) and SASP molecules (PAI-1, IL6,  IL8) in GEnC at early (p9) or late (p17) passage. n = 5 independent experiments. Data information: Data are means ± SEM. Statistical analysis: t-student test"
NCBI gene IDs:
"p21: 1026
p16: 1029
IL8: 3576
IL6: 3569
PAI-1: 26135"
Figure legend: "D. Representative genotyping  of the c.1192C&gt;A mutation by allele-specific PCR. For each sample, two PCRs  were performed, one with a specific primer for the 1192C allele (PCR  1) and a second with specific primers for the 1192A allele (PCR  2). An 821-bp band indicated the presence of the allele; amplification failure indicated the absence of the allele. The 434 bp amplification product of the hGH control primer is presented in all lanes. In the gel, the first lane (MW) shows the 100 bp DNA ladder marker ; for the other lanes, C and A indicate the product from PCR  1 and PCR  2, respectively, amplified from RIF+/+ (1(CC)), RIF+/- (2(CA)) and RIF-/- (3(AA)) samples."
NCBI gene IDs:
"GH: 2688"
Figure legend: "B. Western blotting shows that the anti-RIF  and anti-VER  antibodies bind  to HuSLC44A2. Western blots  of whole Sf9  lysates (WCL) expressing full-length recombinant human SLC44A2  cDNA (rHuSLC44A2) were probed with RIF  and VER  antisera (1:10). Lanes 1 and 2 show strong binding  to rHuSLC44A2. Titer differences in the sera required longer exposure (10 min) of the filter to the film to develop the image with VER  serum compared to only 30 second exposure for RIF  serum. A similar blot  of immunoprecipitated  rHuSLC44A2  from the Sf9  lysates similarly reacted with RIF  and VER  sera (lanes 3 and 4) showed equivalent exposure times (30 seconds for both sera) when tested against the concentrated rHuSLC44A2 protein."
NCBI gene IDs:
"SLC44A2: 57153"
Figure legend: "D. Western blot  of murine Slc44a2  protein immunoprecipitated  from wild-type and Slc44a2  knockout mice  with rabbit anti CTL2-NT serum was probed with anti CTL2-NT (left panel) and with VER  serum (center Panel) or RIF  serum (right panel). The left panel was developed with goat anti rabbit IgG heavy and light chain ; the center and right panels were developed with rabbit anti human IgG and IgM heavy and light chain specific"
NCBI gene IDs:
"Slc44a2: 68682"
Figure legend: "A. Terminal differentiation  of HSPCs  from healthy control and SLC44A2null proband was monitored via α4-integrin and band 3 levels of GPApos cells at day 6 after EPO  addition."
NCBI gene IDs:
"SLC44A2: 57153"
Figure legend: "A. SLC44A2  transcript variants P1 and P2 were amplified from cDNA samples isolated from control reticulocytes  (CTRL RETIC), neutrophils  (CTRL NEUTRO), and PBMC  of the VER- proband 2 (VER PBMC). The purity of reticulocytes  and neutrophils  was verified by flow cytometry using anti-CD71  and anti-CD16  antibodies, respectively."
NCBI gene IDs:
"SLC44A2: 57153"
Figure legend: "(A-H) Live wildtype littermate and Slc7a5-null embryos imaged shortly after dissection. (A-D) Wildtype and (E-H) Slc7a5-null E9.75 embryos from lateral (A, D, E, H), frontal (B, F) or dorsal (C, G) views; (e1-h1) higher magnification images of the Slc7a5-null embryo. White arrowheads indicate the smaller limb bud (compare B and F), open/reduced forebrain (compare D and H), open neural tube at posterior (compare C and E, e1) and anterior (compare B and F, f1) regions. Black arrowheads indicate kinked neural tube (compare C and G, g1) and apparently missing optic and smaller otic vesicles (ov) (compare D and h1). Data information: Scale bars 200 µm"
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "mRNA in situ hybridization and immunofluorescence in E9.5 or E10.5 wildtype and Slc7a5-null embryos for key marker genes. (I-L) Fgf8 mRNA transcripts were detected in wildtype (I-j1) and in Slc7a5-null (K-l1) E10.5 embryos (n=4 each) (white arrowheads indicate limb buds in K and the isthmus in L) with (j1, l1) sections through isthmus at midbrain/hindbrain border and (i1, k1) lateral views of forelimb buds. Data information: Scale bars 200 µm, except sections 100 µm."
NCBI gene IDs:
"Fgf8: 14179
Slc7a5: 20539"
Figure legend: "mRNA in situ hybridization and immunofluorescence in E9.5 or E10.5 wildtype and Slc7a5-null embryos for key marker genes. (M-N) Wholemount Tubulin-β-III (Tuj1) immunofluorescence performed on (M, m1) wildtype and (N, n1) Slc7a5-null E9.5-E10.5 embryos (n=5 each condition). Dorsal root ganglia (DRG) and Sympathetic Chain Ganglia (SCG) are indicated with open arrowheads together with cranial ganglia IX, X and XI. Images in (m1, n1) show higher magnification of the cranial ganglia V, VII/VIII (open arrowheads). Data information: Scale bars 200 µm"
NCBI gene IDs:
"Slc7a5: 20539
Tubulin-β-III: 22152"
Figure legend: "mRNA in situ hybridization and immunofluorescence in E9.5 or E10.5 wildtype and Slc7a5-null embryos for key marker genes. (O-P) Neurog2 expression in wildtype (O-o4) and Slc7a5-null embryos (P-p4) (n= 5 and n=6 each), showing reduced expression in forebrain (o2, p2), cranial ganglia (o1, o3, p1, p3, arrowheads) and in spinal cord at level of forelimb (o4, p4, arrowheads indicate position of neural crest). Data information: Scale bars 200 µm, except sections 100 µm."
NCBI gene IDs:
"Neurog2: 11924
Slc7a5: 20539"
Figure legend: "(A-B) Sox10 in situ hybridization in (A) wildtype and (B) Slc7a5-null E9.5 embryos (n=4 each) with TSs of (a1, b1) the hindbrain at level of trigeminal ganglion V, (a2, b2) otic vesicles and the neural tube at more posterior levels (a3, a4, b3, b4). Scale bars 200 µm, except sections a1 - b4, 100 µm. Closed white arrowheads in (a3, a4) indicate neural crest, open white arrowheads indicate depleted neural crest in (b3) and position where neural crest should be in (b4)."
NCBI gene IDs:
"Slc7a5: 20539
Sox10: 20665"
Figure legend: "(C-G) Immunofluorescence on TSs caudal spinal cord of E9.5 wildtype littermates and Slc7a5-null embryos; Pax3 and FoxA2 were used as indicators for dorso-ventral organisation in (C, F) wildtype, n=2 (for FoxA2) and n= 4 (For Pax3) and (D, G) Slc7a5-null, n=3 (for FoxA2 ) and n = 3 for Pax3) neural tube. Arrowheads indicate border of Pax3 expression domain. Scale bars 50 µm. (E) Percentage of Pax3 expressing cells was determined by counting these cells and all DAPI labelled nuclei in the neural tube and comparison made between wildtype (4 embryos, 6 sections each) and Slc7a5-null (3 embryos, 6 sections each), each dot represents the average for one embryo, unpaired t-test, *p = 0.018 (see original source data). Error bars indicate SEM. "
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "(A-D) Proliferation was assessed in the spinal cord (forelimb level) (A, B) using a phospho-Histone3 (phospho-H3) antibody (green) to identify mitotic cells and DAPI (blue) to label nuclei; cells were counted in 3 wildtype (18 sections) and 3 Slc7a5-null embryos (18 sections) and mitotic index calculated; (C, D) Proliferation was also assessed in the forebrain in 3 wildtype embryos (17 sections) and 3 Slc7a5-null embryos (16 sections). White dashed lines indicate midline. Data information All scale bars 50 µm."
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "(E, F) Comparison of mitotic index in wildtype and Slc7a5-null (E) spinal cord, p = 0.872 ,and (F) forebrain *p = 0.049, each dot represents the average for one embryo, unpaired t-test Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001"
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "(I) Labelling intensity was measured and plotted relative to DAPI intensity; 6 wildtype embryos (18 sections) and 5 Slc7a5-null embryos (19 sections). Each dot represents the average for one embryo, (*p = 0.0457) unpaired t-test with Welch correction Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001"
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "(J) Western blot of individual E9.5 wildtype (n=3) and Slc7a5-null (n=4) embryo lysates immunoblotted using an antibody against phospho-S6K (Thr389) and total P70S6K for loading control. Band intensities were measured with FIJI and an unpaired t-test was performed for statistical analysis Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001"
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "(K-L) (K) Validation by qPCR of the targets identified by RNAseq; qPCR was performed on individual E8.5 embryos of each genotype (wildtype n=5, heterozygous n=9 and Slc7a5-null n=7). (L) qPCR was performed on individual E9.5 embryos (wildtype n=5, heterozygous n=3 and Slc7a5-null n=4) using primers specific for genes associated with the integrated stress response. A one-way Anova test with a Turkey post-test was performed. Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001 Error bars indicate SEM."
NCBI gene IDs:
"Slc7a5: 20539"
Figure legend: "mRNA in situ hybridization was performed at E9.5 to detect Chac1 in (A-a6) wildtype and (B-b6) Slc7a5-null littermate embryos (n=3 for each condition); Chac1 expression at E10 in emerging limb buds assessed in (C) wildtype and (D) Slc7a5-null littermate embryos (n=2 for each condition) Scale bars 200 µm for whole embryo images and 100 µm for sections."
NCBI gene IDs:
"Chac1: 69065
Slc7a5: 20539"
Figure legend: "mRNA in situ hybridization was performed at E9.5 to detect Trib3 mRNA detected in (E-e6) wildtype and (F-f6) Slc7a5-null littermate embryos (n=3 for each condition) and in emerging limb buds at E10.5 assessed in (G) wildtype and (H) Slc7a5-null littermate embryos (n=2 for each condition). Scale bars 200 µm for whole embryo images and 100 µm for sections."
NCBI gene IDs:
"Slc7a5: 20539
Trib3: 228775"
Figure legend: "mRNA in situ hybridization was performed at E9.5 Chac1 and Trib3 transcripts were also detected in wildtype E9.5 embryos after &gt;3 days in situ hybridisation reaction (n=3 and n=6 respectively). Lateral view (I and K), optic vesicles (white arrowheads), branchial arches (ba) and otic vesicles (ov). Dorsal view (J and L), neural tube (nt) and limb buds (white arrowheads). Scale bars 200 µm for whole embryo images and 100 µm for sections."
NCBI gene IDs:
"Chac1: 69065
Trib3: 228775"
Figure legend: "Western blot of individual E9.5 wildtype (n=4) and Slc7a5-null (n=4) embryo lysates immunoblotted using an antibody against phospho-GCN2 (Thr899) and total GCN2 (loading control), ***p = 0.0001 ; band intensities measured with FIJI and analysed with unpaired t-test Data information: All error bars indicate SEM."
NCBI gene IDs:
"Slc7a5: 20539"