EMBO/SourceData huggingface dataset:
Figure legend: "Representative images of the superficial medulla NB layer or deep medulla neuronal layer in the larval or pupal optic lobe, in which UAS-dpn or control (UAS-luciferase) are driven by eyR16F10-GAL4 and stained with the stem cell marker, Miranda (Mira, magenta), (B-E) Mira+ NBs are recovered in superficial sections of control under eyR16F10-GAL4 expression at 6 hr APF and 10 hr APF but not from16 hr APF onwards, quantified in J. (F-I) Mira+ NBs are recovered in deep sections of UAS-dpn driven by eyR16F10-GAL4 at 6 hr APF, 10 hr APF, and 16 hr APF but not at 24 hr APF, quantified in J. Arrow in H points to the NBs. (J) Quantification of % Mira+ cells within eyR16F10-GAL4 expression domain in control and UAS-dpn (calculated as the ratio of Mira+ cell volume as a percentage of total eyR16F10-GAL4 domain volume). 6 hr APF (Control, n= 3, m= 14.82 ±3.521, UAS-dpn, n= 6, m= 18.13 ±1.46), 10 hr APF (Control n= 4, m= 11.65 ±1.605, UAS-dpn n= 5, m= 19.11 ±4.609), 16 hr APF (Control n= 4, m= 0.8897 ±0.239, UAS-dpn n= 7, m= 16.64 ±3.065), 24 hr APF (Control n= 4 m= 1.174, ±0.7226, UAS-dpn n= 6, m= 0.8518 ±0.4849)."
NCBI gene IDs:
"luciferase: 
dpn: 35800
ey: 43812
GAL4: 855828"
Figure legend: "Representative images of the superficial medulla NB layer or deep medulla neuronal layer in the larval or pupal optic lobe, in which UAS-dpn or control (UAS-luciferase) are driven by ok107-GAL4 (outlined) and stained with the stem cell marker, Miranda (Mira, magenta), and mid-temporal progeny marker, Toy-GFP (grey). (K-L') More Toy+ progeny (grey) are present within the ok107-GAL4 domain (green, outlined) in UAS-dpn compared to control."
NCBI gene IDs:
"luciferase: 
dpn: 35800
GAL4: 855828"
Figure legend: "(O-R') Representative images of the deep medulla neuronal  layer in the larval optic lobe, in which heat shock  induced UAS-dpn or control (UAS-luciferase) clones are stained  with the stem cell marker, Mira  (magenta), late  neuronal marker Tll  (grey) or glial  marker Repo  (grey). Arrow points towards the small band of Tll+ neurons  in control clones. (O-P') At 72 hr after clone induction, there is less Tll expression  within UAS-dpn  clones than control clone, quantified in S. (Q-R') At 72 hr after clone induction, there is less Repo expression  within UAS-dpn clones than control clones, quantified in T."
NCBI gene IDs:
"luciferase: 
dpn: 35800"
Figure legend: "Representative images of deep medulla neuronal layer in the larval optic lobe, in which UAS-luc; UAS-dpn and UAS-D; UAS-dpn are expressed in clones by hs flp, and stained with the stem cell marker, Miranda (Mira, magenta), and/or Slp, Tll or Repo (grey). (A-B'') At 72 hr after clone induction, Mira+ NBs within UAS-luc; UAS-dpn clones express Slp; Mira+ NBs within UAS-D; UAS-dpn clones do not express Slp, quantified in I. (C-D') Representative images of deep medulla neuronal layer in the larval optic lobe, in which UAS-dpn and UAS-D; UAS-dpn are expressed in the ok107-GAL4 expression domain, and stained with the stem cell marker, Mira (magenta), and mid-temporal progeny marker, Toy-GFP (grey). Fewer Toy+ cells were recovered within the ok107-GAL4 domain (outlined) in UAS-D; UAS-dpn compared to UAS-dpn, quantified in E. (E) Quantification of % Toy+ cells within ok107-GAL4 expression domain in UAS-dpn and UAS-D; UAS-dpn (calculated as the ratio of Toy+ cell volume as a percentage of total ok107-GAL4 domain volume). UAS-dpn n= 9, m= 22.78 ±4.174, UAS-D; UAS-dpn n= 5, m= 14.42 ±2.394."
NCBI gene IDs:
"luc: 
dpn: 35800
GAL4: 855828"
Figure legend: "(J-K) Quantification of % Tll+ or Repo+ cells within UAS-dpn clones compared to UAS-D; UAS-dpn clones (calculated as the ratio of Tll+ or Repo+ cell volume as a percentage of total clone volume). Tll (UAS-dpn n= 7, m= 0.7198 ±0.2389, UAS-D; UAS-dpn n= 9, m= 35.22 ±4.917), Repo (UAS-dpn n= 7 m= 1.488 ±0.2368, UAS-D; UAS-dpn n= 9, m= 14.13 ±2.672)."
NCBI gene IDs:
"dpn: 35800"
Figure legend: "(L-M) Mira+ NBs are present in deep sections of UAS-dpn driven by eyR16F10-GAL4 at 126h but are absent in UAS-D; UAS-dpn, quantified in N. (N) Quantification of % Mira cells within eyR16F10-GAL4 expression domain in UAS-dpn and UAS-D; UAS-dpn (calculated as the ratio of Mira+ cell volume as a percentage of total eyR16F10-GAL4 domain volume). UAS-dpn n= 6, m= 18.13 ±1.46, UAS-D; UAS-dpn n= 8, m= 1.859 ±0.4215. D"
NCBI gene IDs:
"dpn: 35800
ey: 43812
GAL4: 855828"
Figure legend: "(A-C'') Representative images of the deep medulla neuronal layer in the larval optic lobe, in which control (UAS-luc) and UAS-dpn and UAS-D; UAS-dpn are expressed in hs flp clones (72 hr after clone induction). Miranda (Mira, magenta) and EdU (grey). Despite increased number of Mira+ cells in UAS-dpn compared to control, each NB underwent slower cell cycle progression, as indicated by EdU+ incorporation. Quantified in E. This slow cell cycle speed was not rescued in UAS-D; UAS-dpn clones. Quantified with UAS-luc; UAS-dpn in F."
NCBI gene IDs:
"luc: 
dpn: 35800"
Figure legend: "(G-H''') Representative images of the deep medulla neuronal layer in the larval optic lobe, where UAS- E2f1; UAS- CycE; UAS-dpn or UAS-dpn are expressed in the ok107-GAL4 domain (outlined), and stained with the stem cell marker, Mira (magenta), mid-tTF, Slp (grey) and late progeny marker, Repo (Cyan). Quantified in I-K. UAS-dpn NBs express Slp and do not create Repo+ progeny, whereas UAS- E2f1; UAS- CycE; UAS-dpn NBs do not express Slp and create Repo+ progeny."
NCBI gene IDs:
"CycE: 34924
dpn: 35800
E2f1: 42550
GAL4: 855828"
Figure legend: "(B-D''') Representative images of the deep medulla neuronal layer in the larval optic lobe, in which control (UAS-luc), UAS-NACT, and UAS-D; UAS-NACT are driven by eyR16F10-GAL4 (outlined). Miranda (Mira, magenta), Sloppy paired (Slp, grey), Tailless (Tll, cyan). UAS-NACT NBs express Slp and not Tll. UAS-D; UAS-NACT NBs express Slp and Tll, quantified in E-H."
NCBI gene IDs:
"luc: 
ey: 43812
GAL4: 855828"
Figure legend: "(J-K') Representative images of the deep medulla neuronal layer in the larval optic lobe, in which UAS-NACT and UAS-NACT; UAS-dpn are driven by ok107-GAL4 (marked by GFP and outlined) and stained with the stem cell marker, Mira (magenta), and mid-temporal progeny marker, Toy-GFP (grey). Fewer cells express Toy+ in UAS-NACT; UAS-dpn compared to UAS-NACT, quantified in I."
NCBI gene IDs:
"GFP: 
dpn: 35800
GAL4: 855828"
Figure legend: "(A-C''') Representative images of the deep medulla neuronal layer in the larval optic lobe, in which control (UAS-lacz), lola-Ri, and UAS-D; lola-Ri are driven by eyR16F10-GAL4 (outlined). Miranda (Mira, magenta), Sloppy-paired (Slp, grey), Twin of eyeless (Toy, cyan). lola-Ri induces ectopic Mira+ NBs in the deep medulla layers, compared to control, quantified in E."
NCBI gene IDs:
"ey: 43812
GAL4: 855828
lacz: 945006
lola: 44548"
Figure legend: "(H-J''') Representative images of the deep medulla neuronal layer in the larval optic lobe, in which control (UAS-lacz), lola-Ri, and UAS-D; lola-Ri are driven by eyR16F10-GAL4 (outlined). Miranda (Mira, magenta), Tailless (Tll, grey), Reversed polarity (Repo, cyan). lola-Ri NBs express less Tll compared to control. There are fewer Tll+ and Repo+ progeny in the eyR16F10-GAL4 domain in lola-Ri compared to control. UAS-D; lola-Ri NBs express more Tll as well as more Tll+ and Repo+ progeny in eyR16F10-GAL4 domain compared to lola-Ri alone, quantified in K-L."
NCBI gene IDs:
"ey: 43812
GAL4: 855828
lacz: 945006
lola: 44548"
Figure legend: "Images of representative individuals (F) of 4-day-old seedlings grown with or without supplemental UV-B. The scale bar for all lines represent 5 mm except for cop1-5 where the scale bars represent 1 mm. Data Information , lines used: wild-type (Ws), uvr8-7, cop1-4, cop1-5/Pro35S:YFP-COP1 (WT), cop1-5/Pro35S:YFP-COP1Lys422Ala, cop1-5/Pro35S:YFP-COP1Tyr441Ala, cop1-5/Pro35S:YFP-COP1Trp467Ala and cop1-5. #1 and #2: independent transgenic lines."
NCBI gene IDs:
"YFP: 
cop1-5: 817857
COP1: 817857
cop1-4: 817857
uvr8: 836506"
Figure legend: "quantification of hypocotyl lengths (G) of 4-day-old seedlings grown with or without supplemental UV-B. The scale bar for all lines represent 5 mm except for cop1-5 where the scale bars represent 1 mm. Violin and box plots are shown for n &gt; 60 seedlings; upper and lower hinges correspond to the first and third quartiles, the horizontal line in the interior of the box indicates the median. Data Information: lines used: wild-type (Ws), uvr8-7, cop1-4, cop1-5/Pro35S:YFP-COP1 (WT), cop1-5/Pro35S:YFP-COP1Lys422Ala, cop1-5/Pro35S:YFP-COP1Tyr441Ala, cop1-5/Pro35S:YFP-COP1Trp467Ala and cop1-5. #1 and #2: independent transgenic lines."
NCBI gene IDs:
"YFP: 
cop1-5: 817857
COP1: 817857
cop1-4: 817857
uvr8: 836506"
Figure legend: "Quantitative real-time PCR analysis of (H) HY5 expression. Four-day-old seedlings grown in white light were exposed to narrowband UV-B for 2 hours (+UV-B), or not (-UV-B). Error bars represent SEM of 3 biological replicates. Data Information: lines used: wild-type (Ws), uvr8-7, cop1-4, cop1-5/Pro35S:YFP-COP1 (WT), cop1-5/Pro35S:YFP-COP1Lys422Ala, cop1-5/Pro35S:YFP-COP1Tyr441Ala, cop1-5/Pro35S:YFP-COP1Trp467Ala and cop1-5. #1 and #2: independent transgenic lines."
NCBI gene IDs:
"YFP: 
cop1-4: 817857
cop1-5: 817857
COP1: 817857
HY5: 830996
uvr8: 836506"
Figure legend: "Quantitative real-time PCR analysis of (I) RUP2 expression. Four-day-old seedlings grown in white light were exposed to narrowband UV-B for 2 hours (+UV-B), or not (-UV-B). Error bars represent SEM of 3 biological replicates. Data Information lines used: wild-type (Ws), uvr8-7, cop1-4, cop1-5/Pro35S:YFP-COP1 (WT), cop1-5/Pro35S:YFP-COP1Lys422Ala, cop1-5/Pro35S:YFP-COP1Tyr441Ala, cop1-5/Pro35S:YFP-COP1Trp467Ala and cop1-5. #1 and #2: independent transgenic lines."
NCBI gene IDs:
"YFP: 
COP1: 817857
cop1-4: 817857
cop1-5: 817857
RUP2: 832438
uvr8: 836506"
Figure legend: "F. Yeast 3-hybrid analysis of the COP1 - HY5 interaction in the presence of UVR8. (Top) Normalized Miller Units were calculated as a ratio of β-galactosidase activity in yeast grown under UV-B (+ UV-B) versus yeast grown without UV-B (- UV-B). Additionally, normalized Miller Units are reported separately here for yeast grown on media without or with 1 mM methionine, corresponding to induction (- Met) or repression (+ Met) of Met25 promoter-driven UVR8 expression, respectively. Means and SEM for 3 biological repetitions are shown. (Bottom) representative filter lift assays. AD, activation domain; BD, DNA binding domain; Met, methionine."
NCBI gene IDs:
"UVR8: 836506"
Figure legend: "B. Representative image showing the phenotype of wild-type (Ws), uvr8-7 and uvr8-7/Pro35S:UVR8HY5C44, uvr8-7/Pro35S:UVR8HY5VP and uvr8-7/Pro35S:UVR8TRIB1 seedlings grown for 4 days under white light (- UV-B) or white light supplemented with UV-B (+ UV-B). The scale bar represents 5 mm."
NCBI gene IDs:
"TRIB1: 10221
uvr8: 836506
UVR8: 836506"
Figure legend: "E. Quantitative real-time PCR analysis of RUP2, CHS, HY5 and ELIP2 expression in wild-type (Ws), uvr8-7 and uvr8-7/Pro35S:UVR8HY5C44, uvr8-7/Pro35S:UVR8HY5VP and uvr8-7/Pro35S:UVR8TRIB1 4-day old seedlings grown under white light in response to 2h of UV-B (+UV-B vs. -UV-B). Error bars represent SEM of 3 biological replicates. Note: the primers used to detect the HY5 transcript abundance also bind to an identical region present in UVR8HY5C44 chimera."
NCBI gene IDs:
"ELIP2: 827119
HY5: 830996
RUP2: 832438
TRIB1: 10221
CHS: 831241
uvr8: 836506
UVR8: 836506"
Figure legend: "F. Yeast 3-hybrid analysis of the COP1-HY5 interaction in the presence of the UVR8HY5C44 chimera. (Left pane) Normalized Miller Units were calculated as a ratio of β-galactosidase activity in yeast grown under UV-B (+ UV-B) versus yeast grown without UV-B (- UV-B). Additionally, normalized Miller Units are reported separately here for yeast grown on media without or with 1 mM methionine, corresponding to induction (- Met) or repression (+ Met) of Met25 promoter-driven UVR8 expression, respectively. Means and SEM for 3 biological repetitions are shown. (Right panel) Representative filter lift assays of the yeast analyzed in left panel. AD, activation domain; BD, DNA binding domain; Met, methionine."
NCBI gene IDs:
"UVR8: 836506"
Figure legend: "A. Representative image of wild-type (Ws), cop1-5/Pro35S:YFP-COP1, cop1-5/Pro35S:YFP-COP1Lys422Ala, cop1-5/Pro35S:YFP-COP1Tyr441Ala and cop1-5/Pro35S:YFP-COP1Trp467Ala transgenic lines grown for 39 days in long day conditions."
NCBI gene IDs:
"YFP: 
cop1-5: 817857
COP1: 817857"
Figure legend: "B. Representative image of individual wild-type (Ws), co-11, cop1-5/Pro35S:YFP-COP1 and cop1-5/Pro35S:YFP-COP1Lys422Ala plants grown for 39 days in long days conditions."
NCBI gene IDs:
"YFP: 
co: 831441
COP1: 817857
cop1-5: 817857"
Figure legend: "E. Immunoblot analysis of CRY2 and actin (loading control) protein levels in wild-type (Col), cry2-1, wild-type (Ws), cop1-5/Pro35S:YFP-COP1 and cop1-5/Pro35S:YFP-COP1Lys422Ala seedlings grown for 4 days in darkness."
NCBI gene IDs:
"YFP: 
COP1: 817857
cop1-5: 817857
cry2-1: 839529"
Figure legend: "E Representative fluorescent stereo-micrographs of glands injected with MM134:RFP-luc2  cells (left, top and bottom) infected with either GFP:sh-scramble (top) or GFP+ shLOXL1  (bottom), n=5 and 3. Scale bar, 1000 μm. F Barplot showing the ratio of GFP/RFP signal from fluorescent stereo-micrographs  of glands engrafted with MM134  cells, n=8. Statistical analysis by paired Student t-test, two-tailed. *p &lt; 0.05. "
NCBI gene IDs:
"GFP: 
luc2: 
RFP: 
LOXL1: 4016"
Figure legend: "H Representative  fluorescent stereo-micrographs  of glands engrafted with SUM44:RFPluc2 cells infected with either GFP:sh-scramble (top) or GFP+ shLOXL1, n=6. Scale bar, 1000 μm. I Barplot (mean ± SEM) showing quantification of the ratio GFP/RFP  from fluorescent stereo-micrographs of SUM44. Statistical analysis by unpaired Student's t-test, two-tailed, n=7 sh-scramble and n=6 for sh-LOXL1, ****p &lt; 0.0001. "
NCBI gene IDs:
"GFP: 
luc2: 
RFP: 
LOXL1: 4016"
Figure legend: "J, K Representative photomicrographs of picrosirius red stained  histological sections for sh-scramble or sh-LOXL1 MM134 (J) and SUM44 (K) xenografts. Scale bars, 100 μm."
NCBI gene IDs:
"LOXL1: 4016"
Figure legend: "(K) HEK-293T cells were transfected with Flag-NLRP3 or Flag-vector and then treated with LicoB (40 μM). Immunoprecipitation was performed with anti-DYKDDDDK (Flag) affinity gel agarose beads; Western blot analysis has been shown."
NCBI gene IDs:
"Flag: 
NLRP3: "
Figure legend: "Under the same growth conditions (permitted water, free food, and a 12 h/12 h dark/light cycle at 20±2°C), eight-week-old male C57BL/6 mice were continuously fed with methionine- and choline-supplemented (MCS) or methionine- and choline-deficient (MCD) diets for 6 weeks, and at the same time, gavaged with LicoB, MCC950 n=6 mice per group Real-time quantitative PCR was used to detect the mRNA levels of Col1a1 (E), TNF-α (F) in the mice livers, as described in (A) (n=6 mice per group)."
NCBI gene IDs:
"Col1a1: 12842
TNF-α: 21926"
Figure legend: "Under the same growth conditions (permitted water, free food, and a 12 h/12 h dark/light cycle at 20±2°C), eight-week-old male C57BL/6 mice were continuously fed with methionine- and choline-supplemented (MCS) or methionine- and choline-deficient (MCD) diets for 6 weeks, and at the same time, gavaged with LicoB, MCC950 n=6 mice per group Real-time quantitative PCR was used to detect the mRNA levels of IL-1β (G), and IL-18 (H) in the mice livers, as described in (A) (n=6 mice per group)."
NCBI gene IDs:
"IL-18: 16173
IL-1β: 16176"
Figure legend: "A-C qPCR of selected transcripts at day 31 to validate (A) enrichment of cardiac markers in GFP+ fractions against GFP− fractions, (B) upregulation of atrial and (C) downregulation of ventricular genes in RA+ compared to CT+ (n = 3).Data information: Data are presented as mean ± SEM. In (A-C), *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by unpaired t-test. In (A), for TNNT2, P = 0.0001 for CT− against CT+ and P = 0.0002 for RA− against RA+; for NKX2.5, P = 0.00005 for CT− against CT+ and P = 0.00007 for RA− against RA+. In (B), P = 0.0006 for NPPA and P = 0.0002 for PITX2. In (C), P = 0.02 for HEY2 and P = 0.007 for IRX4."
NCBI gene IDs:
"HEY2: 23493
IRX4: 50805
NKX2.5: 1482
NPPA: 4878
PITX2: 5308
TNNT2: 7139"
Figure legend: "A, B Line plot illustrating relative mRNA levels of (A) COUP-TFI and (B) COUP-TFII in VM and AM differentiations from day 5 through day 9 (left) and in GFP+CMs at day 31 (right); n = 3.Data information: Data are presented as mean ± SEM. In (A, B), *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by unpaired t-test. In (A), left panel, P = 0.03, 0.02, 0.03, 0.005 and 0.0004 for comparison of COUP-TFI expression between AM and VM at days 5, 6, 7, 8 and 9 of differentiation. In (A), right panel, P = 0.0002 for comparison of COUP-TFI expression at day 31 between AM and VM. In (B), left panel, P = 0.02, 0.03, 0.006, 0.004 and 0.003 for comparison of COUP-TFII expression between AM and VM at days 5, 6, 7, 8 and 9 of differentiation. In (B), right panel, P = 0.0001 for comparison of COUP-TFII expression at day 31 between AM and VM. CT = control differentiation; hESC-atrial (AM); hESC-ventricular (VM)."
NCBI gene IDs:
"COUP-TFI: 7025
COUP-TFII: 7026"
Figure legend: "A, B mRNA expression of COUP-TFI and COUP-TFII following shRNA-mediated knockdown of (A) COUP-TFI or (B) COUP-TFII in hESC-atrial cardiomyocytes (AM) at day 30."
NCBI gene IDs:
"COUP-TFI: 7025
COUP-TFII: 7026"
Figure legend: "C, D mRNA expression of ion channel genes KCNA5, KCNJ3 and KCNJ5 after knockdown of (C) COUP-TFI or (D) COUP-TFII in AM at day 30."
NCBI gene IDs:
"KCNA5: 3741
KCNJ3: 3760
KCNJ5: 3762
COUP-TFI: 7025
COUP-TFII: 7026"
Figure legend: "E, F Schematic of NR2F binding sites in (E) KCNA5 and (F) KCNJ3 promoters.G, H ChIP-qPCR analysis at day 30 shows enriched binding of COUP-TFI and COUP-TFII to the promoter region of (G) KCNA5 and (H) KCNJ3, compared to IgG in AM."
NCBI gene IDs:
"KCNA5: 3741
KCNJ3: 3760"
Figure legend: "A Expression of KCNA5 (left) and KCNJ3 (right) in GFP+ pools of VM and AM CMs at day 31, as well as in ventricles and atria of human heart."
NCBI gene IDs:
"KCNA5: 3741
KCNJ3: 3760"
Figure legend: "D. Log-normalized expression  z-score of genes up- (top panels) and downregulated (bottom panels) by Ascl1   (left, blue violins) and MyoD1   (right, yellow violins) versus their scaled expression  levels (x-axis) binned into 10% intervals. For each box, the centerline defines the median, the height of the box is given by the interquartile range (IQR), the whiskers are given by 1.5 * IQR and outliers are given as points beyond the minimum or maximum whisker."
NCBI gene IDs:
"Ascl1: 17172
MyoD1: 17927"
Figure legend: "F. Representative immunofluorescence  images of cells 3 days after transgene induction. Cells were stained  for the fluorescent reporter present on the PiggyBac construct of the reprogramming factor. Arrows indicate  multinucleated cells. Scale bar represents 50 µm. mutAscl1 = mutant Ascl1. G. Quantification of the percentage of cells with more than one nucleus  among all transfected cells within a given condition. Data points represent biological replicates (n = 4). For each box, the centerline defines the median, the height of the box is given by the interquartile range (IQR), the whiskers are given by 1.5 * IQR and outliers are given as points beyond the minimum or maximum whisker. Pairwise comparisons performed with Mann-Whitney U test, correction for multiple testing performed with Benjamini-Hochberg correction. *: p &lt; 0.05 (MyoD1     vs Ascl1: p = 0.03, MyoD1     vs mutAscl1: p = 0.03, MyoD1     vs Ascl1     &amp; MyoD1: p = 0.03, MyoD1     vs mutAscl1 &amp; MyoD1: p = 0.03, Ascl1     vs Ascl1     &amp; MyoD1: p = 0.03, Ascl1     vs mutAscl1  &amp; MyoD1: p = 0.03, mutAscl1   vs Ascl1    &amp; MyoD1: p = 0.03, mutAscl1  vs mutAscl1   &amp; MyoD1: p = 0.03"
NCBI gene IDs:
"Ascl1: 17172
MyoD1: 17927"
Figure legend: "(B-C) The phospholipases pla2g4aa  and pla2g4ab  are highly expressed  in neutrophils. Data information: For each panel, data represents biological triplicates plated in technical duplicates. Statistical analysis was completed using one-way ANOVA, multiple comparison test. * P&lt;.05; **P&lt;.01; ***P&lt;.001; ****P&lt;.0001. Center values denote the mean, and error bars denote s.e.m."
NCBI gene IDs:
"pla2g4aa: 30554
pla2g4ab: 559087"
Figure legend: "(D-E-F) The expression  of cyclooxygenases cox1, cox2a  and cox2b  is enriched in macrophages. (G-H) The prostaglandin synthases ptges3a  and ptges3b  are only expressed  by ECs. (I-O) The prostaglandin receptors ptger1a, ptger1b, ptger2a, ptger3, ptger4a  are mostly expressed  in HSPCs. (P-Q) The prostaglandin transporters slco2b1  and abcc4  are specifically expressed  in ECs. Data information: For each panel, data represents biological triplicates plated in technical duplicates. Statistical analysis was completed using one-way ANOVA, multiple comparison test. * P&lt;.05; **P&lt;.01; ***P&lt;.001; ****P&lt;.0001. Center values denote the mean, and error bars denote s.e.m."
NCBI gene IDs:
"abcc4: 368620
cox1: 140539
ptger1a: 559315
ptger1b: 564945
ptger2a: 393608
ptger3: 100000544
ptger4a: 562469
ptges3a: 406449
ptges3b: 415227
cox2a: 246227
cox2b: 559020
slco2b1: 792084"
Figure legend: "(A) Schematic indicating the imaging area in the tail  at 72hpf, as indicated by the black box; fluorescence imaging of the CHT  in double transgenic cd45:CFP-NTR/runx1:mcherry embryos  in DMSO  and after treatment with MTZ  and PGH2  or PGE2. (B) Quantification of runx1:mcherry  positive-cells (C) Quantification of cd45:CFP  positive-cells in double transgenic cd45:CFP-NTR/runx1:mcherry embryos  in DMSO  and after treatment with MTZ  and PGH2 or PGE2. Data information: Statistical analysis was completed using one-way ANOVA, multiple comparison test. * P&lt;.05; **P&lt;.01; ***P&lt;.001 ****P&lt;.0001. Scale bar 200μm (A)."
NCBI gene IDs:
"mcherry: 
CFP: 
NTR: 945778
cd45: 559154
runx1: 12394"
Figure legend: "(A) WISH for cmyb expression  at 60hpf in wild type and slco2b1-/- embryos. (B) Quantification of cmyb-expressing cells. Each n represents the number of cmyb-expressing cells for each embryo  (biological replicates). Each experiment has been repeated three independent times. Data information: Centre values denote the mean, and error values denote s.e.m. The statistical analysis was completed using an unpaired two tailed t test. ***P &lt; .001 Scale bar 500μm (A)"
NCBI gene IDs:
"cmyb: 30519
slco2b1: 792084"
Figure legend: "(C) Schematic indicating the imaging area in the tail  at 60hpf, as indicated by the black box; fluorescence imaging in the CHT  of kdrl:mCherry;cmyb:GFP embryos  injected with control- and slco2b1-Mos. (D) Quantification of HSPCs  associated to ECs. Each n represents the number of yellow spots for each embryo  (biological replicates). Each experiment has been repeated three independent times. Data information: Centre values denote the mean, and error values denote s.e.m. The statistical analysis was completed using an unpaired two tailed t test. ***P &lt; .001 Scale bar 200μm (C)"
NCBI gene IDs:
"slco2b1: 792084"
Figure legend: "(E) Experimental outline and quantification of time-lapse live imaging  in controls and slco2b1-morphants  cmyb:GFP+ cells, using Imaris software. Data information: The statistical analysis was completed using an unpaired two tailed t test. ***P &lt; .001"
NCBI gene IDs:
"slco2b1: 792084"
Figure legend: "(A) Anti-GFP  and pH3 immunostainings  of either controls (Ctrl-Mo) or slco2b1-morphants (slco2b1-MO) cmyb:GFP embryos. (B) Quantification of the number of the pH3+ HSCs  in controls or slco2b1-morphants. Centre values denote the mean, and error values denote s.e.m, statistical analysis was completed using an unpaired two tailed t test. ***P &lt; .001. Data information: Scale bar 50μm (A)"
NCBI gene IDs:
"slco2b1: 792084"
Figure legend: "(D) Quantification of PGE2   concentration in control and slco2b1   morphants. The statistical analysis was completed using an unpaired two tailed t test *P &lt; .01. Centre values denote the mean, and error bars denote s.e.m."
NCBI gene IDs:
"slco2b1: 792084"