EMBO/SourceData huggingface dataset:
Figure legend: "Ubiquitination analysis of immunoprecipitated TSC2 (E) from activated (anti-CD3 plus anti-CD28 for 2 h) wildtype or Peli1-KO CD8 T cells by immunobloting using anti-ubiquitin Abs detecting total (Ub), K48 (Ub-K48), or K63 (Ub-K63) polyubiquitin chains."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Ubiquitination analysis of immunoprecipitated TSC1 (F) from activated (anti-CD3 plus anti-CD28 for 2 h) wildtype or Peli1-KO CD8 T cells by immunobloting using anti-ubiquitin Abs detecting total (Ub), K48 (Ub-K48), or K63 (Ub-K63) polyubiquitin chains."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "L Co-immunoprecipitation analysis of endogenous TSC1-TSC2 interaction in wildtype and Peli1-KO CD8 T cells that were either not treated (NT) or stimulated for 2 h with anti-CD3 plus anti-CD28. The right panel is a summary graph of TSC2/TSC1 ratio based on quantification of the blot from anti-TSC1 IP (the right four lanes of the top two panels)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "A Analysis of ubiquitin conjugation to wildtype (WT) TSC1 and the indicated TSC1 mutants in 293T cells transiently transfected Flag-tagged TSC1 and its mutants along with (+) or without (-) the indicated expression vectors."
NCBI gene IDs:
"Flag: 
TSC1: 7248"
Figure legend: "B Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in lysates of TSC1-KO MEFs transfected with (+) or without (-) TSC1 wildtype or K30A expression vectors. The cells were serum starved and then left either untreated (NT) or stimulated with 10% FBS for 30 mins. Data are presented as a representative blot (left panel) and a summary graph of quantified TSC2 (right panel) protein bands relative to the level of β-Actin."
NCBI gene IDs:
"TSC1: 64930
TSC1: 7248"
Figure legend: "D Co-immunoprecipitation analysis of the interaction between endogenous TSC2 and exogenous TSC1 wildtype and K30A in TSC1-KO MEFs transfected (for 48 h) with (+) or without (-) the indicated expression vectors. KO MEF cells were transfected with indicated expression plasmids. The right panel is a summary graph of TSC2/TSC1 ratio based on quantification of the last four lanes of the anti-Flag IP blot in left panel."
NCBI gene IDs:
"TSC1: 64930"
Figure legend: "E Analysis of ubiquitin conjugation to endogenous TSC2 in TSC1-KO MEFs transfected (for 48 h) with (+) or without (-) TSC1 WT or K30A and treated with MG132 for 2 h before lysis."
NCBI gene IDs:
"TSC1: 64930
TSC1: 7248"
Figure legend: "F Immunoblot analysis of endogenous TSC2 in TSC1-KO MEFs transfected (for 48 h) with Myc-TSC1 wildtype or K30A and subsequently treated with cycloheximide (10 μg/ml) for the indicated time periods before lysis. The right panel is a summary graph of quantified TSC2 WT and K30A protein bands presented as percentages of the level in the untreated (0 min) lane."
NCBI gene IDs:
"Myc: 
TSC1: 64930
TSC1: 7248"
Figure legend: "A. The unfertilized oocytes were injected with spermatozoa with a variant in IQCN at 15-18 h post-injection. The white arrowheads represent the polar body (PB). PN represents pronucleus."
NCBI gene IDs:
"IQCN: 80726"
Figure legend: "A. Fertility assessment experiments in WT and Iqcn-/- male mice after mating with WT female mice (n = 18)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "B. Size of the testes and epididymides in WT and Iqcn-/- male mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "C. Quantification ratio of the testis weight/body weight in WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "D. Sperm counts in the caudal epididymis from WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "E. H&amp;E staining of sperm from WT and Iqcn-/- male mice. Red arrowheads represent sperm with no sickle shape heads."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "F. Percentage of sperm without sickle shape heads from WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "G. Ultrastructure of spermatozoa from WT and Iqcn-/- male mice. Ac represents the acrosome."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "H. Immunostaining of lectin (green) in spermatozoa from WT and Iqcn-/- male mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "I. Percentage of abnormal acrosomes in spermatozoa from WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "J. Percentage of spontaneous and induced acrosome reactions in spermatozoa from WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "A. Representative two pronucleus (2PN) zygotes, two-cell embryos, and blastocysts after ICSI in WT and Iqcn-/- male mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "B. Percentage of 2PN zygotes, two-cell embryos and blastocysts after ICSI in WT and Iqcn-/- male mice (n = 3)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "C. Profile of Ca2+ responses induced by oocyte injection with spermatozoa from WT and Iqcn-/- male mice (n = 5). Spermatozoa from WT male mice induced 8-9 Ca2+ spikes over a 3-h period (blue line), while spermatozoa from Iqcn-/- male mice failed to induce spikes (red line)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "D. Immunostaining of oocytes injected with spermatozoa from WT and Iqcn-/- male mice. The expression of PLCζ (red) and α-tubulin (green) was detected in oocytes. M represents male nucleus and F represents the female nucleus."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "E. Expression and localization of PLCζ in sperm from WT and Iqcn-/- male mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "F. Expression and localization of PLCζ during acrosomal biogenesis from WT and Iqcn-/- male mice. PLCζ was localized in the acroplaxome in WT spermatids. PLCζ was only localized in the tip of the head in the cap and acrosome phases (white arrowheads) and was lost during the maturation phase in Iqcn-/- mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "B. Ultrastructure of the testes from WT and Iqcn-/- male mice from steps 8-16. MA represents manchette. PR represents perinuclear ring. CD represents cytoplasmic droplets. Scale bars, 2 μm (steps 8-12; 15-16) and 5 μm (steps 13-14)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "D. Length of the manchette structure in spermatids at steps 13-14 from WT and Iqcn-/- male mice (n = 10)."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "A. Expression and localization of calmodulin during spermiogenesis in the testes of WT and Iqcn-/- male mice. Calmodulin (red) was co-localized with α-tubulin (green) from steps 8-14 in WT mice, but failed to co-localize in steps 13-14 in Iqcn-/- male mice."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "B. Interaction between IQCN and calmodulin (CaM) following co-IP by IQCN antibody in testes from WT and Iqcn-/- male mice (n = 3). IQCN interacts with calmodulin in WT mice, the interaction of which is completely lost in Iqcn-/- male mice. GAPDH was used as the internal control."
NCBI gene IDs:
"Iqcn: 637079"
Figure legend: "C. Immunostaining of calmodulin (red) and α-tubulin (green) in HeLa cells. The normal (WT) and mutant (c.910C&gt;T and c.2453_2454del) plasmids of IQCN were transfected into HeLa cells. HeLa cells were treated with an antagonist of calmodulin W-7. Multipolar spindles were observed in HeLa cells transfected with mutant plasmids and W-7 treatment."
NCBI gene IDs:
"IQCN: 80726"
Figure legend: "D. Percentage of multipolar spindles in WT and mutant IQCN transfected HeLa cells. (n = 3 biological replicates)"
NCBI gene IDs:
"IQCN: 80726"
Figure legend: "(B-F) Basal views through living SCs, with dashed white circles approximating the outline of a single SC, and acidic compartments marked by the vital dye LysoTracker® Red (magenta). In merge images, a single non-acidic (C, D, F) and acidic (E) compartment containing intraluminal vesicles (ILVs) is boxed and magnified in the right panel (Zoom). ILVs appear as membrane-delineated vesicles, using super-resolution 3D-structured illumination (3D-SIM) microscopy for the brighter overexpressed GFP-tagged constructs (yellow; C and F). However, ILVs appear only as puncta, using lower resolution wide-field microscopy for the fainter endogenously expressed YFP-tagged Rab GTPases (yellow; D and E). (B) Wide-field fluorescence image, including differential interference contrast (DIC), of SC expressing a GFP-tagged version of human CD63 (CD63-GFP). CD63-GFP expression is apparent on the limiting membranes of non-acidic compartments and their ILVs, and also on the limiting membranes of the enlarged acidic compartments. Most large non-acidic compartments are Rab11-positive (D) and contain dense-core granules, which have a 'fried egg' appearance with DIC (arrowheads) (Corrigan et al., 2014; Redhai et al., 2016). (C) 3D-SIM image of CD63-GFP-expressing SC. Arrow highlights CD63-GFP-marked ILVs (Zoom). Many more ILVs are apparent in non-acidic compartments in a complete Z-stack of a non-acidic compartment (Movie EV1). (D) Wide-field fluorescence image of an SC expressing a YFP-Rab11 gene trap. YFP-Rab11 marks the limiting membranes of most non-acidic compartments and internal puncta (arrow in Zoom), but not the surface of acidic compartments (Appendix Fig S1B). (E) Wide-field fluorescence image of SC expressing a YFP-Rab7 gene trap. YFP-Rab7 marks the limiting membranes of acidic compartments and internal puncta (arrow in Zoom). Enlarged acidic compartments are also present in adjacent main cells. (F) 3D-SIM image of SC expressing a GFP-tagged version of Breathless (Btl-GFP). Btl-GFP marks the limiting membranes of non-acidic compartments and their ILVs (arrow in Zoom), but not the surface of acidic compartments (Appendix Fig S1C). Images from six-day-old male flies shifted to 29°C at eclosion. This induces GAL4/UAS-dependent SC transgene expression in (B), (C) and (F). The genotypes of flies carrying multiple transgenes are: w; P[w+, UAS-CD63-GFP] P[w+, tub-GAL80ts]/+; dsx-GAL4/+ (B, C); w; P[w+, tub-GAL80ts]/+; dsx-GAL4/P[w+, UAS-btl-GFP] (F)."
NCBI gene IDs:
"CD63: 
GAL4: 
GAL80: 
GFP: 
tub: 
YFP: 
Breathless: 39564
Btl: 39564
btl: 39564
CD63: 967
dsx: 40940
Rab11: 42501
Rab7: 42841"
Figure legend: "(A-D) Wide-field fluorescence images of basal views through living SCs expressing a GFP-tagged form of Btl-GFP (yellow) in non-acidic compartments, with SC outline approximated by dashed white circles. Acidic compartments are marked by LysoTracker Red® (magenta). Boxed non-acidic compartments in merge images are magnified in A-D, Zoom. On the right, lower magnification confocal transverse images of fixed accessory gland (AG) lumens are shown with dotted lines indicating the basal side of the AG epithelial layer, which contains several fluorescent SCs (highlighted by arrows). (A) SC with no RNAi construct expressed (control) and AG lumen from same genotype. Btl-GFP-positive compartments containing fluorescent ILVs (in Btl-GFP panel), ILV membranes inside compartments (in Zoom panel) and secreted fluorescent puncta (AG lumen panel) are marked by arrowheads. (B) SC also expressing RNAi construct targeting ESCRT-0 subunit, Stam, and AG lumen from same genotype. Btl-GFP-positive ILVs (Zoom) and secreted puncta (AG lumen) are strongly reduced. (C) SC also expressing RNAi construct targeting ESCRT-I subunit, Vps28, and AG lumen from same genotype. Btl-GFP-positive ILVs (Zoom) and secreted puncta (AG lumen) are strongly reduced. (D) SC also expressing RNAi construct targeting ESCRT-III subunit, shrb, and AG lumen from same genotype. Btl-GFP-positive ILVs (Zoom) and secreted puncta are strongly reduced. Genotypes are: w; P[w+, tub-GAL80ts]/+; dsx-GAL4/P[w+, UAS-btl-GFP] with no knockdown construct (A), UAS-Stam-RNAi (HMS01429; B), UAS-Vps28-RNAi (v31894; C) or UAS-shrb-RNAi (v106823; D)."
NCBI gene IDs:
"GAL4: 
GAL80: 
GFP: 
tub: 
Btl: 39564
btl: 39564
dsx: 40940
shrb: 35933
Stam: 34505
Vps28: 47408"
Figure legend: "(E) Super-resolution 3D-SIM image of fixed HCT116 cell expressing GFP-Rab11a (yellow), and stained with CD63 (magenta). DAPI (grey) marks nucleus. Boxed Rab11a-positive compartments, which frequently cluster, are magnified in Merge Zoom. This panel is further magnified in Merge Zoom x 2, revealing GFP-Rab11a (arrows in right panel) inside compartments."
NCBI gene IDs:
"GFP: 
Rab11a: 8766"
Figure legend: "(F) Wide-field fluorescence image of fixed HCT116 cells, stained with Rab11a (yellow) and Rab7 (magenta), expressing constitutively active GFP-tagged Rab5 (GFP-Rab5CA; cyan), which stalls endosomal maturation and produces enlarged Rab5-positive endosomes. One of these is boxed in the Merge and magnified in Merge Zoom, revealing internal puncta marked by Rab11a (arrows) and Rab7 (arrowheads) and limiting membrane subdomains of Rab11a (yellow arrowhead) and Rab7 (magenta arrowhead). DAPI (grey) marks nuclei."
NCBI gene IDs:
"GFP: 
Rab5: 5868"
Figure legend: "Western blot analyses of EV preparations. Gel loading is normalised to cell lysate protein levels. Bar charts present changes in levels of putative exosome proteins relative to cell lysate protein (D) EVs isolated by UC from HCT116 cells cultured for 24 h following three days of raptor or non-targeting (NT) shRNA knockdown."
NCBI gene IDs:
"raptor: 57521"
Figure legend: "(C) Western blot analysis of EVs isolated by UC from HCT116 cells cultured in glutamine-replete and -depleted medium for 24 h, following transduction with a Rab11a or control non-targeting (NT) shRNA knockdown construct over previous two days. Bar chart shows change in putative exosome proteins in EVs secreted from Rab11a knockdown cells relative to NT-treated cells in glutamine-depleted conditions, following initial normalisation to cell lysate protein. (C') Growth curves are for HCT116 recipient cells pre-treated with EVs isolated as in (C) or with vehicle (PBS). ****P colour denotes significant increase relative to EVs from glutamine-replete cells (black) and Rab11a knockdown cells (red)."
NCBI gene IDs:
"Rab11a: 8766"
Figure legend: "(F) Western blot analysis of EVs isolated by UC from HCT116 cells in glutamine-replete medium, transduced with a Rab7 or non-targeting (NT) control shRNA knockdown construct. Bar charts show changes in putative exosome proteins in EVs isolated by ultracentrifugation, following normalisation to cell lysate protein. (F') Growth curves are for HCT116 recipient cells pre-treated with EVs isolated as in (F) or with vehicle (PBS)."
NCBI gene IDs:
"Rab7: 42841"
Figure legend: "(A) A northern blot depicting a time course experiment ranging from five minutes to 24 hours of MCF10A cells in response to oxidative stress. A single probe complementary to pre-tRNATyrGUA, mature tRNATyrGUA, and tRFTyrGUA expression was 32P-labeled and used for detection. (B) Quantification of pre-tRNATyrGUA (left) and mature tRNATyrGUA levels (right) by northern blot analysis from multiple independent experiments (normalized to U6 levels) are shown (n=6). "
NCBI gene IDs:
"U6: "
Figure legend: "(C) MCF10A cells were exposed to oxidative stress (200μM H2O2) once daily for five continuous days. (D) Quantification of mature tRNATyrGUA bands by northern blot after cells were treated once daily for five continuous days (normalized to U6) from multiple independent experiments (n=12). "
NCBI gene IDs:
"U6: "
Figure legend: "(C) Western blot of MCF10A expressing a control short-hairpin RNA or a hairpin targeting the tyrosine-tRNA synthetase, YARS (red arrow). HSC70 was used as a loading control."
NCBI gene IDs:
"tyrosine-tRNA synthetase: 8565"
Figure legend: "(D) Growth curves of MCF10A cells expressing RNAi targeting mature tRNATyrGUA or YARS relative to cells expressing a control hairpin (n=3). Two-way ANOVA was used to test for significance."
NCBI gene IDs:
"YARS: 8565"
Figure legend: "(A) Cells depleted of tRNATyrGUA or YARS were processed for label free quantitation by mass spectrometry to identify proteins that were reduced by a log2-fold change of 0.5 or more. This set was overlapped with proteins containing a higher than median abundance of Tyr codon content to identify candidate mediators of the pleiotropic effects of tRNATyrGUA depletion."
NCBI gene IDs:
"YARS: 8565"
Figure legend: "(E) Growth curves for MCF10A cells were transfected with either control siRNA or two independent siRNA targeting EPCAM, SCD, or USP3. Note that the control cell growth curve is the same in all graphs and were plotted separately for clarity and does not represent independent experiments. Two-way ANOVA was used to test for significance (n=3)."
NCBI gene IDs:
"EPCAM: 4072
SCD: 6319
USP3: 9960"
Figure legend: "(E) A cumulative distribution in control and hnRNPA1 depleted cells of the stability levels for mRNA transcripts with 3' UTR hnRNPA1 CLIP binding (Huelga et al., 2012). Transfection of tRFTyrGUA led to a significant right-shift in the expression levels of 3' UTR bound hnRNPA1 transcripts. Statistical significance was measured using the Kolmogorov-Smirnov test. (F) Cumulative distribution as in (E). Transfection of locked nucleic acid against tRFTyrGUA and treatment with 200µM H2O2 led to a significant left-shift in mRNA stability of 3'UTR bound hnRNPA1 transcripts. Statistical significance was assessed using the Kolmogorov-Smirnov test. "
NCBI gene IDs:
"hnRNPA1: 3178"
Figure legend: "(H, I) Representative H&amp;E (H) and dystrophin immunofluorescence of images of TA muscle cross-sections in WT and Prmt5MKO mice. Scale bar: 100 μm."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(J, Distribution of myofiber size (J) of WT (n=4) and Prmt5MKO TA muscles (n=4)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(L) The total number of myofibers in EDL and Soleus from WT (n=5) and Prmt5MKO mice (n=6)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(B-D) Exercise performance of maximum speed (B), running time (C), running distance (D) of WT (n=11) and Prmt5MKO mice (n=9) measured by treadmill."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(E, F) Absolute (E) and specific force (F) (on the left panel) and maximal absolute and specific force (on right panel) on EDL muscle from WT (n=4) and Prmt5MKO mice (n=3). (G, H) Absolute (G) and specific force (H) (on the left panel) and maximal absolute and specific force (on right panel) on Soleus muscle from WT (n=4) and Prmt5MKO mice(n=3)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(A) Representative immunostaining of Type I, IIA, and IIB myofibers in in EDL and Soleus muscles of 2-month-old WT and Prmt5MKO mice. (B, C) Quantification of abundance of various fiber types in EDL (B) and Soleus (C) muscles of WT and Prmt5MKO mice; (n=5)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(D) Representative histochemical staining image of succinate dehydrogenase (SDH) enzymatic activity in EDL and Soleus muscles. (E, F) Quantification of abundance of SDH-low, -medium and -high myofibers in EDL (E) and Soleus (F) muscles of WT (n=5) and Prmt5MKO mice (n=4)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(G, H) Metabolic rate of O2 consumption (G) and CO2 production (H) normalized to lean mass for a 48-hour cycle of 4-6-month-old WT (n=8) and Prmt5MKO (n=7) mice measured by an indirect calorimetry."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(A, B) Representative images of ORO staining (A) and immunofluorescence (B) in TA muscle from WT and Prmt5MKO mice. Scale bar: 100 μm."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(C) Western blotting analysis for protein markers of lipolysis, lipogenesis, and electron transport chain (ETC) complexes in skeletal muscles of WT (n=3) and Prmt5MKO mice; (n=3)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(A) C2C12 cells overexpressing PRMT5-GFP alone or PRMT5-GFP + SREBP1a-Flag were immunoprecipitated with Flag antibody and blotted with SYM10, GFP, Flag, PRMT5, and Tubulin antibodies. (B) C2C12 cells overexpressing SREBP1a-Flag alone or SREBP1a-Flag + PRMT5-GFP were immunoprecipitated with GFP antibody and blotted with Flag, GFP, PRMT5, and Tubulin antibodies."
NCBI gene IDs:
"Flag: 
GFP: 
PRMT5: 10419
SREBP1a: 6720"
Figure legend: "(E) HEK293 cells were transfected with PRMT5-GFP alone or PRMT5-GFP + SREBP1a-Flag for 24 hours, followed by cycloheximide (30 μg/ml) and protein analysis at 0, 4, 8 hours. Lysates were immunoblotted with Flag, GFP, and tubulin antibodies."
NCBI gene IDs:
"Flag: 
GFP: 
PRMT5: 10419
SREBP1a: 6720"
Figure legend: "(G) Relative expression of lipogenesis genes (Dgat1, Dgat2, Fabp4) in C2C12 transfected with PRMT5 and SREBP1a; (n=4, technical replicates)."
NCBI gene IDs:
"Dgat1: 13350
Dgat2: 67800
Fabp4: 11770
PRMT5: 10419
SREBP1a: 6720"
Figure legend: "(A, B) Immunoblots showing symmetric demethylation of H4R3 in skeletal muscles of WT (n=4) and Prmt5MKO mice (n=4) (A), and quantification of H4R3Me2s normalized to total H4 (B)."
NCBI gene IDs:
"Prmt5: 27374"
Figure legend: "(E) ChIP-sequencing results showing the Prmt5 binding peak on the Pnpla2 promoter of 3T3-L1 cells."
NCBI gene IDs:
"Pnpla2: 66853"
Figure legend: "(H, I) Enrichment of PRMT5 (H) and H4R3Me2s (I) on the proximal promoter region of the Pnpla2 gene in skeletal muscles of WT and Prmt5MKO mice (n=4 for H, and I, biological replicates)."
NCBI gene IDs:
"Pnpla2: 66853
Prmt5: 27374"
Figure legend: "(D) Immunoblotting analysis showing PRMT5, ATGL and ETCs in muscle tissues (upper panel) and quantification of its relative protein levels in muscles of WT, Prmt5MKO, and Prmt5/Pnpla2MKO mice (bottom panel) (n=2, biological replicates)."
NCBI gene IDs:
"Pnpla2: 66853
Prmt5: 27374"
Figure legend: "(E, F) Exercise performance in running distance (E) and griping strength test (F) in WT (n=11 for E, n=8 for F), Prmt5MKO (n=10 for E, n=9 for F) and Prmt5/Pnpla2MKO mice (n=9 for E, n=8 for F)."
NCBI gene IDs:
"Pnpla2: 66853
Prmt5: 27374"
Figure legend: "(I) Representative images of ORO staining in TA muscles under brightfield (upper panel) and fluorescent imaging (bottom panel) from WT, Prmt5MKO, and Prmt5/Pnpla2MKO mice. Scale bar: 50 μm."
NCBI gene IDs:
"Pnpla2: 66853
Prmt5: 27374"
Figure legend: "(b) A53T α-synuclein (α-syn) expression was induced in stable PC12 cells for 48 h, then switched off for 24 h. Cells were treated with DMSO (control), or with 0.94 μM, 4.7 μM or 47 μM of SMER10; 0.86 μM, 4.3 μM or 43 μM of SMER18; or 0.9 μM, 4.7 μM or 47 μM of SMER28 added in the switch-off period. A53T α-syn levels were analyzed by immunoblotting with antibody against HA (left) and densitometry analysis relative to actin (right). Error bars denote s.e.m. P = 0.0917, P = 0.009, P = 0.0001 (for increasing concentrations of SMER10); P = 0.0068, P = 0.0023, P = 0.0002 (for increasing concentrations of SMER18); P = 0.0016, P 0.0001, P 0.0001 (for increasing concentrations of SMER28)."
NCBI gene IDs:
"α-synuclein: 6622"
Figure legend: "(d) Wild-type (Atg5+/+) and knockout (Atg5−/−) Atg5 MEFs were transfected with EGFP-HDQ74 and treated with DMSO (control), 47 μM SMER10, 43 μM SMER18 or 47 μM SMER28 for 24 h, and we assessed the percentages of aggregate-containing transfected cells. The control (DMSO-treated) values were fixed at 1 for both cell lines. Error bars: 95% confidence interval. P = 0.039 (SMER10), P 0.0001 (SMER18 and SMER28) (in Atg5+/+ cells); P = 0.092 (SMER10), P = 0.271 (SMER18), P = 0.358 (SMER28) (in Atg5−/− cells). Note that EGFP-HDQ74 aggregation was higher in Atg5−/− than in Atg5+/+ cells (Supplementary Fig. 3a)."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(a) COS-7 cells transfected with EGFP-LC3 construct for 4 h were treated with DMSO (control), 0.2 μM rapamycin (positive control), 47 μM SMER10, 43 μM SMER18 or 47 μM SMER28 for 16 h, and analyzed by fluorescence microscopy. The effects of treatment on the percentage of EGFP-positive cells with &gt;5 EGFP-LC3 vesicles are shown. Error bars denote s.e.m. P 0.0001 (all SMERs)."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "(b) HeLa cells stably expressing EGFP-LC3 were treated with DMSO (control), 47 μM SMER10, 43 μM SMER18 or 47 μM SMER28 for 24 h. Confocal sections show cells containing EGFP-positive autophagic vesicles. Nuclei are stained with DAPI. Bar, 20 μM."
NCBI gene IDs:
"LC3: 440738///81631///84557"