EMBO/SourceData huggingface dataset:
Figure legend: "D-J. MCU silencing reduces mRNA levels of HIF-1α, HIF-2α, and HIF-1α target genes. Cells were transfected with siMCU or siControl. mRNA expression was measured by RealTime-PCR (n=3). P-values: (HIF-1α) **p=0.0031, **p=0.009, (HIF-2α)**p=0.01, (LOX)***p=0.001, ***p=0.0005, (PDK1)*p=0.02, **p=0.009, (G6PI)*p=0.02, **p=0.005, (CA IX)**p=0.0026, **p=0.0022, (HK2) **p=0.0024, *p=0.03."
NCBI gene IDs:
"CA IX: 768
HIF-2α: 2034
G6PI: 2821
HIF-1α: 3091
HK2: 3099
LOX: 4015
MCU: 90550
PDK1: 5163"
Figure legend: "K. HIF-1α overexpression rescues siMCU-mediated migration impairment. Cells were transfected with siMCU or siControl. Wild type (wt) and constitutively active (ca) HIF-1α were expressed by retroviral infection (pBABE was used as control). The day after transduction, cells were scratched (time-point 0h). Cell migration into the wounded area was monitored at 48 hour time-point and the covered area was measured (n=12). P-values: ***p&lt;0.0001, *p=0.04."
NCBI gene IDs:
"HIF-1α: 3091
MCU: 90550"
Figure legend: "L-M. MCU expression levels correlate with HIF1α (L) and HIF1α-regulated genes (M). A linear model (lm) to test the power of MCU expression levels predicting the expression of HIF1α and HIF1α-regulated genes was calculated and plotted for each of the 532 samples of the TCGA database (see Figure 1). Equation and R2 values of the linear regression and significance indicating deviation from 0 are shown. The area of 95% prediction limit is shaded below and above the linear regression line. The HIF1α-regulated gene set was compiled from Broad Institute GSEA database (http://www.broadinstitute.org/gsea/msigdb/cards/V$HIF1_Q5.html, merged sets of V$HIF1_Q3 and V$HIF1_Q5)."
NCBI gene IDs:
"HIF1α: 3091
MCU: 90550"
Figure legend: "B HeLa cells expressing the indicated GFP-tagged Nups were semi-permeabilized and incubated with mitotic HeLa cell extract at 30ºC. NPC disassembly was followed by monitoring the fluorescence intensity of the respective Nups at the nuclear rim and influx of TRITC-dextran. Scale bar, 30 μm."
NCBI gene IDs:
"GFP: "
Figure legend: "D, E Detection of FLAIL and asFLAIL gene expression in Col-0 and flail mutants by strand-specific RT-qPCR. Transcript levels were normalized to UBQ expression levels. Y-axis showed relative values compared to the expression level of Col-0."
NCBI gene IDs:
"FLAIL: 
flail: 
UBQ: 830705"
Figure legend: "B, C Detection of sense FLAIL and asFLAIL transcript expression in Col-0, flail3 mutant and complementation lines by strand-specific RT-qPCR. pFLAIL:gFLAIL18/88 and pasFLAIL:gasFLAIL18/39 represented sense and antisense FLAIL complementation lines, respectively with flail3 35S:GUS transgenic line as a control. Positions of primers used for strand-specific RT-qPCR analyses were the same as those in Figure 1A. Transcript levels were normalized to UBQ expression levels. Y-axis showed relative values compared to the expression level of Col-0."
NCBI gene IDs:
"FLAIL: 
flail: 
GUS: 
UBQ: 830705"
Figure legend: "D Representative morphological phenotypes of 4-week-old plants of Col-0, flail3 mutant, transgenic lines. Scale bar: 2 cm. E Violin graph showed number of rosette leaves after appearance of the first flower bud in Col-0."
NCBI gene IDs:
"flail: "
Figure legend: "A Heatmap of 13 flowering genes and sense FLAIL was cropped with systematic gene names illustrating that the expression of dysregulated flowering genes in flail3 was fully (AGL24, CO, COOLAIR, CIR1, FBH4, LAC8, PLL25, PRE1) or partially (AGL20, DDF1, MAF4, NMT3, PHYA) rescued in complementation line. DEGs analyzed by DESeq2 with |log2 fold change| &gt; 1 and adjusted p value &lt; 0.05 were considered significant differential expression. Three biological replicates for Col-0, flail3, and two for flail3 pFLAIL:gFLAIL18 (complement). Flowering genes were highlighted in red. The color scale reflected the log 2-fold change in gene expression, ranging from down-regulated (blue) to up-regulated (red)."
NCBI gene IDs:
"FLAIL: 
flail: 
AGL20: 819174
AGL24: 828556
NMT3: 843694
PLL25: 827005
COOLAIR: 28720403
CO: 831441
DDF1: 837817
FBH4: 818829
LAC8: 831877
MAF4: 836631
PHYA: 837483
PRE1: 833982
CIR1: 833700"
Figure legend: "Genome browser screenshots illustrating the expression of sense FLAIL and dysregulated flowering genes in flail3 were rescued in complementation line (top panel). Normalized read counts (TPM from RNA-seq) for differentially expressed flowering genes in Col-0, flail3, and flail3 pFLAIL:gFLAIL18 plants (bottom panel)."
NCBI gene IDs:
"FLAIL: 
flail: "
Figure legend: "E, F Genome browser screenshots illustrating two of FLAIL bound targets CIR1 (E) and LAC8 (F) in ChIRP-seq."
NCBI gene IDs:
"FLAIL: 
LAC8: 831877
CIR1: 833700"
Figure legend: "FLAIL affects AS of LAC8. Light blue arrows indicate probes used for gel electrophoresis in C; LAC8 isoforms were detected by RT-PCR with black arrow indicating LAC8.1, grey arrow indicating LAC8.2, and brown arrow indicating LAC8.3. -RT, negative control without adding reverse transcriptase. Isoform bands were quantified using Image Lab software relative to the respective UBQ band of each sample."
NCBI gene IDs:
"FLAIL: 
UBQ: 830705
LAC8: 831877
LAC8.1: 831877
LAC8.2: 831877
LAC8.3: 831877"
Figure legend: "F-H ChIRP-qPCR using probe pools FLAIL-asDNA (Odd and Even) identified FLAIL binding snRNAs U1, U2 and U5. Luc-asDNA probes retrieved RNAs as a negative control. Retrieved FLAIL transcript was served as a positive control; UBQ and a non-coding, unspliced, conserved snoRNA lncCOBRA1 (AT1G05913) as negative controls"
NCBI gene IDs:
"FLAIL: 
Luc: 
AT1G05913: 28716398
COBRA1: 28716398
UBQ: 830705
U5: 836235
U2: 829822
U1: 824230"
Figure legend: "I Morphological phenotypes of 4-week-old plants of Col-0, flail3, lac8-52-34, lac8 flail3-55-1 mutants. Scale bar: 2 cm. J Violin graph showed number of rosette leaves after appearance of the first flower bud in Col-0. Data represented the mean of at least seven independent experiments in Col-0, flail3, lac8-52-34, lac8 flail3-55-1."
NCBI gene IDs:
"flail: 
lac8: 831877"
Figure legend: "Volcano plots of piliation, aggregation and adhesion mutation scores for all pilE mutations. Stop and synonymous mutations are highlighted in blue and orange respectively. Adhesion and aggregation mutation scores relative to piliation. The correlation appears as a red dotted line."
NCBI gene IDs:
"pilE: "
Figure legend: "Early adhesion of pilE mutants as a function of aggregation. Values were normalized to that of pilESB. Each dot represents a pilE mutant and the pilV, pilD, pilX and pilC1 reference mutants. Mutants in the region surrounding A131 are in green and those surrounding Y50 in purple. A blown-up view of the zone of interest (lower left quarter) is presented on the right panel. The shaded area highlights the absence of significant adhesion."
NCBI gene IDs:
"pilE: "
Figure legend: "Early adhesion of PilE mutants as a function of piliation per bacterium normalized to that of pilESB."
NCBI gene IDs:
"PilE: "
Figure legend: "C-terminally GFP-tagged NRC2EEE and C-terminally RFP-tagged Rx were co-expressed either with an EV-4xMyc construct or a CP-4xMyc construct in leaves of nrc2/3/4 N. benthamiana CRISPR mutant lines. (A-B) Single-plane confocal micrographs show the localization of both components of the inactive and active Rx-NRC2 system, together with PM marker RPW8.2-BFP. Scale bars represent 10 µm. (A) NRC2EEE-GFP and Rx-RFP co-localize in the cytoplasm prior to activation. (B) Upon co-expression of CP and activation of the system, NRC2EEE forms puncta associated with the PM while Rx remains in the cytoplasm."
NCBI gene IDs:
"CRISPR: 
Myc: 
nrc2: 
CP: 7065758"
Figure legend: "(C) Membrane enrichment assays are consistent with microscopy, showing that inactive NRC2EEE-GFP is mostly present in the soluble (cytoplasmic) fraction, whereas activated NRC2EEE-GFP exhibits equal distribution across soluble and membrane fractions. Rx is mostly present in the soluble fraction and exhibits no change upon activation of the system with CP. T = total, S = soluble, M = membrane. ATPase was used as a membrane marker. Rubisco was used as a marker for total and soluble fractions and visualized by Ponceau staining (PS). Red asterisks indicate bands matching the expected MW for each protein. The experiment was repeated two times."
NCBI gene IDs:
"CP: 7065758"
Figure legend: "(A-B) Single-plane confocal micrographs show the localization of NRC2 together with Rx or free RFP during PVX infection. C-terminally GFP-tagged NRC2EEE was co-infiltrated with either EV-RFP or Rx-RFP in leaves of nrc2/3/4 N. benthamiana CRISPR mutant lines and infected with PVX by agroinfection. Scale bars represent 10 µm. (A) In the absence of Rx, NRC2EEE-GFP is localized to the cytoplasm during PVX infection. (B) When Rx is present, NRC2EEE forms puncta associated with the PM during PVX infection."
NCBI gene IDs:
"CRISPR: 
nrc2: "
Figure legend: "A-C 10x106 ZsGreen- and luciferase-expressing U-2932, RC-K8 and RIVA cells were intravenously injected into six-week-old male (M) and female (F) MISTRG mice and monitored weekly using IVIS for at least four and up to six weeks. The color scales on the right indicate the radiance, i.e. the sum of the photons per second from each pixel inside the ROI/number of pixels (photons/sec/cm2/sr)."
NCBI gene IDs:
"luciferase: 
ZsGreen: "
Figure legend: "10x106 ZsGreen- and luciferase-expressing U-2932, RC-K8 and RIVA cells were intravenously injected into six-week-old male (M) and female (F) MISTRG mice and monitored weekly using IVIS for at least four and up to six weeks. D The frequency of involvement of the indicated tissues is shown for one cohort of mice and is representative of two independently analyzed cohorts per cell line."
NCBI gene IDs:
"luciferase: 
ZsGreen: "
Figure legend: "E Gating strategy for the identification of U-2932 cells residing in spleen and bone marrow, among all leukocytes in these organs. Cells were stained for human and mouse CD45. Very few hCD45+ U-2932 cells were detectable in the spleen, whereas their frequencies were much higher in the bone marrow (BM). In BM, &gt;90% of U-2932 cells were positive for ZsGreen. The green curve shows U-2932 cells recovered from BM, overlayed with unlabelled U-2932 cells grown in vitro (grey curve)."
NCBI gene IDs:
"ZsGreen: "
Figure legend: "MISTRG mice were reconstituted or not at birth with human CD34+ cord blood HSPCs, and intravenously injected with RC-K8 cells at six weeks of age. The lymphoma burden at the study endpoint was quantified by ZsGreen expression and in the case of RC-K8 cells, also over time by IVIS (H)."
NCBI gene IDs:
"ZsGreen: "
Figure legend: "The expression of the IL-6Rα (CD126) and signaling chains (gp130) was assessed by SYBR or Taqman qRT-PCR (B, C) respectively, on a panel of DLBCL cell lines. ABC- and GCB-DLBCL cell lines are color-coded in red and green; RC-K8 are depicted in grey, as their subtype is a matter of debate"
NCBI gene IDs:
"CD126: 3570
IL-6Rα: 3570
gp130: 3572"
Figure legend: "E STAT3 phosphorylation as assessed by Western blotting of CD126-proficient (WT) or -deficient (ko) RC-K8 cells, with and without 1h of exposure to 50ng/ml hIL-6."
NCBI gene IDs:
"CD126: 3570"
Figure legend: "F Kinetics of STAT3 phosphorylation as assessed by Western blotting, of the IL-6-expressing SOCS1wt and SOCS1mut cell lines OCI-Ly3 and SU-DHL-2, under 1, 6 and 24h exposure to an IL-6-neutralizing antibody (α-IL-6, anti-IL-6 neutralizing antibody)."
NCBI gene IDs:
"SOCS1: 8651"
Figure legend: "(F, G) Ribo-seq and RNA-seq  expression data of Cand2  in sham and TAC operated animals (CPM - count per million). One-way ANOVA, ** - P ≤ 0.01, n=2-4 biological replicates per group. Data information: Error bars indicate means ± SEM"
NCBI gene IDs:
"Cand2: 192226"
Figure legend: "(J) Schematic representation of reporter consisting of human Cand2 5'UTR and downstream  Renilla luciferase coding sequence. A potential TOP-like motif is highlighted in a grey box. Black arrows indicate transcription start sites in the adult heart  according to the Database of Transcriptional Start Sites (Release 10.1). The effect of Cand2  5'UTR on translation measured by luciferase activity in normal conditions and after  mTORC1  block with Torin 1. eEF2 - positive control of reporter with defined 5'TOP motif and non-TOP 5'UTR of  β-actin used as a negative control. Total protein content in lysates used for  luciferase assay. t-test, n=4-5 biological replicates per group, ** - P ≤ 0.01, *** - P ≤ 0.001."
NCBI gene IDs:
"luciferase: 
β-actin: 60
Cand2: 23066
mTORC1: 56717"
Figure legend: "(C)  Immunofluorescent staining of paraffin  sections from hearts  from WT and Cand2 KO mice. CAND2 (stained in red), ACTIN (stained in green) and nuclei (stained with  DAPI, blue) in. Scale bar 20μm"
NCBI gene IDs:
"Cand2: 67088"
Figure legend: "(E) NRCMs size measurement after Cand2 OE and 24h PE stimulation. Analyzed by One-way ANOVA. n&gt;150 cells from n=3 independent experiments. Violin plot: the middle horizontal lane - median, upper lane - 3rd quartile, lower lane - 1st quartile. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Cand2: 192226"
Figure legend: "(F) Nppa mRNA levels in vitro after  Cand2  OE and PE treatment measured by RT-qPCR. Analysed by One-way ANOVA. n=5-9 biological replicates per conditions. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Cand2: 192226
Nppa: 24602"
Figure legend: "(J) Nppa and Nppb  mRNA levels 4 weeks after TAC in WT and Cand2  KO  mice measured by RT-qPCR. Analyzed by One-way ANOVA. n=6-13 mice per group. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001 "
NCBI gene IDs:
"Cand2: 67088
Nppa: 230899
Nppb: 18158"
Figure legend: "(B)  Immunoblots  and quantification of Grk5 protein levels in vitro after Cand2 KD with siRNA and after Cand2  OE with AAV6. t-test, n=4-5 biological replicates per group. Data information: Error bars indicate means ± SEM. * - P ≤ 0.05, *** - P ≤ 0.001."
NCBI gene IDs:
"Cand2: 192226"
Figure legend: "(F) Representative  immunoblot  of Grk5 protein levels and quantification in cytoplasmic  and  nuclear fractions from left ventricle lysates of WT and Cand2 KO mice after TAC surgery. Lamin B - nuclear  marker, Gapdh  - cytoplasmic marker. Analyzed by One-way ANOVA, n=3 biological replicates. Data information: Error bars indicate means ± SEM. * - P ≤ 0.05, *** - P ≤ 0.001. "
NCBI gene IDs:
"Cand2: 67088"
Figure legend: "(B) mRNA levels of MEF2-dependent genes Nr4a1  and Xirp2  in NRCMs with Cand2 KD for indicated groups. PE treatment for 24h (50uM). Analyzed by One-way ANOVA, n=6 biological replicates. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Cand2: 192226
Nr4a1: 79240
Xirp2: 311098"
Figure legend: "(I) Grk5, Nppa, and MEF2  targets  Nr4a1  and Xirp2  mRNA levels in NRCMs  after  Raptor  KD. Analyzed by t-test, n=3 biological replicates per conditions. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Grk5: 59075
Nppa: 24602
Nr4a1: 79240
Raptor: 287871
Xirp2: 311098"
Figure legend: "(B)  Cul1 neddylation  analyzed by  Western blot in NRCMs  depleted of  Cand2. NRCMs were treated with MLN4924 (100 nM) to block Cul1 neddylation. Quantification of neddylated  and unnedylated  Cul1  protein levels after MLN4924  treatment for 24h. t-test, n=4 biological replicates. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Cand2: 192226"
Figure legend: "(D) Grk5  expression in NRCMs upon Cand2 KD and Cul1 neddylation inhibition (MLN) analysed by  immunoblot  and RT-qPCR. Analyzed by One-way ANOVA, n=4-5 biological replicates. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Cand2: 192226"
Figure legend: "(H) mRNA levels of MEF2-dependent genes Nr4a1 and Xirp2 in  NRCMs for indicated groups. Analyzed by One-way ANOVA, n= 6 biological replicates. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001, **** - P ≤ 0.0001"
NCBI gene IDs:
"Nr4a1: 79240
Xirp2: 311098"
Figure legend: "(C) Grk5 protein levels in vivo in LVs of WT and Cand2  KO mice 2 days after sham and TAC analyzed by  immunoblotting as well as quantification of mRNA levels analyzed by RT-qPCR. Analyzed by One-way ANOVA. n=3-5 biological replicates per group. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001."
NCBI gene IDs:
"Cand2: 67088"
Figure legend: "(D) Representative  immunoblot  and its quantification of protein levels of neddylated  and unnedylated  Cul1 in Cand2 KO  mice  compared to WT mice. Analyzed by t-test. n=5-8  mice per group. Cul1  neddylation was inhibited in C2C12 by MLN4924 (-/+ MLN) for neddylated Cul1 band detection. Data information: Error bars indicate means ± SEM; * - P ≤ 0.05, ** - P ≤ 0.01, *** - P ≤ 0.001."
NCBI gene IDs:
"Cand2: 67088"
Figure legend: "(a) Relative CK2α protein concentration over time following cytoplasmic microinjection of REF52 cells with the HA-tagged semisynthetic proteins bearing different modifications using immunocytochemistry with HA-specific antibody. Data represent mean values ± s.d. (b) Relative CK2α protein concentration following injection of β subunit together with CK2α proteins. Data represent mean values ± s.d. (c) Relative CK2α protein concentration following injection of proteasome inhibitor (1 μM MG132) together with unmodified CK2α protein. Data represent mean values ± s.d."
NCBI gene IDs:
"CK2α: 1457"
Figure legend: "(a) Pull-down experiments with semisynthetic HA-CK2α protein and recombinant Pin1. CK2α was immobilized using HA-specific (anti-HA) antibodies bound to protein G beads. Pin1 was incubated with immobilized CK2α for 20 min at 4 °C."
NCBI gene IDs:
"CK2α: 1457
Pin1: 5300"
Figure legend: "(b) Coimmunoprecipitation of endogenous Pin1 with CK2α. Pfa-containing and unmodified semisynthetic HA-CK2α proteins were spiked into REF52 cell lysates and immobilized using HA-specific antibodies. Input samples are shown for loading control."
NCBI gene IDs:
"CK2α: 1457"
Figure legend: "(c) Pull-down experiments with Pfa344 HA-CK2α protein and recombinant Pin1 in the presence of Pin1-specific or nonspecific IgY."
NCBI gene IDs:
"Pin1: 5300"
Figure legend: "(d) Relative CK2α protein concentration over time following microinjection of Pfa344 CK2α with Pin1-specific IgY or nonspecific IgY (control). Data represent mean values ± s.d. Full gels corresponding to those in panels a-c are in Supplementary Figures 10 and 11."
NCBI gene IDs:
"CK2α: 1457
Pin1: 5300"
Figure legend: "Phosphorimage analysis of in vitro CK2 kinase assays with protein substrates. All reactions were performed at 30 °C. Numbers indicate the concentration (nM) of product for each kinase reaction. (a) AHCYL2 substrate (25 ng μl−1) with 100 μM ATP and 10 nM CK2α ± 11 nM CK2β for 6 min. (b) NAP1L3 substrate (15 ng μl−1) with 100 μM ATP and 10 nM CK2α ± 11 nM CK2β for 6 min. (c) NIPBL substrate (50 ng μl−1) with 100 μM ATP and 5 nM CK2α ± 11 nM Pin1 for 5 min. Full gels corresponding to those in panels a-c are in Supplementary Figures 19 and 25."
NCBI gene IDs:
"CK2α: 1457
CK2β: 1460
Pin1: 5300"
Figure legend: "(s,t) Quantitative real-time PCR of orfs. 19.4325, 19.130, RIC1 on cDNA from Candida SC5314 (s) or the TOR1-1/TOR1 mutant (t) strains exposed to vehicle (open column), 10 ng ml−1 IL-17A (black column) or 15 nM rapamycin (grey column) for 4 h. The actual P-values are indicated (two-tailed t-test) (n=3)."
NCBI gene IDs:
"TOR1: 
TOR1-1: 
19.4325: 3637818
RIC1: 3639409
19.130: 3644040"
Figure legend: "(w) orf. 19.5258 expression after 24-h Candida SC5314 exposure as in (n,o). Data are mean±s.d. (n=3). The actual P-values are indicated (two-tailed t-test)."
NCBI gene IDs:
"19.5258: 3642025"
Figure legend: "(p-r) Cytospins of vaginal fluids from IL-17F-deficient (p), IL-17A-deficient (q) and C57BL/6 (r) mice intravaginally infected two days before with C.albicans SC5314 blastospores. Note the presence of hyphae in the vagina of IL-17F-deficient mice after May-Grünwald-Giemsa staining (scale bars, 20 μm). Shown is one representative experiment out of three."
NCBI gene IDs:
"IL-17A: 16171
IL-17F: 257630"
Figure legend: "(c) Failure of IL-17A to bind to wild-type (red square) or mutant C. albicans strains in which the three genes of the CRH family (UTR2, open light blue, CRH1, open black and CRH12, open blue square) had been deleted, and restoration of binding by CRH1 (black square) and CRH12 (blue square), but not UTR2 (light blue square), reconstitution. Data are mean±s.d. (n=3). P0.001 for red square, open light blue, black square and blue square (one-way analysis of variance Bonferroni post-test)."
NCBI gene IDs:
"CRH12: 
CRH1: 3638085
UTR2: 3636591"
Figure legend: "(d,e) Cellular localization of bound IL-17A. Electron micrographs of ultrafine sections of C. albicans MKY 378 wild-type (d) or the crh11Δ mutant (e) incubated with biotinylated IL-17A and streptavidin gold-conjugated particles. Arrows indicate immunogold particles. Scale bars, 200 nm."
NCBI gene IDs:
"crh11: "
Figure legend: "Fungi were harvested from the stomachs of C57BL/6 (a-c), IL-17F-deficient (d-f) or IL-17A-deficient (g-i) mice infected with GFP-Candida for 2 days, and stained using PE-labeled antibody to IL-17A. Cells were green in (a,d and g), red upon staining with anti-IL17A-PE (b,e and h) and double stained in the merged images (c,f and i). Scale bars, 10 μm. Images are from one representative experiment out of two. (j-l) SEM images showing fungal germination in the stomachs of C57BL/6 mice (j), filamentation in IL-17F-deficient mice (k) and neither one in IL-17A-deficient (l) mice intragastrically infected 2 days before. Scale bars, 10 μm. (a-l) Images are from one representative experiment out of two."
NCBI gene IDs:
"IL-17A: 16171
IL-17F: 257630"
Figure legend: "(m-o) C57BL/6 mice were infected intragastrically with C. albicans MKY 378 strain (m) or the crh11Δ (n) and crh12Δ (o) mutants, and were monitored for fungal growth (log10 colony-forming units (CFU) at 2 (open circle) or 8 (black circle) days post infection). Data are mean±s.d. (n=3). The actual P-values are indicated (two-tailed t-test) for 2 and 8 days (m) and for 2 days (o). Fungal cells were untreated (none) or exposed to 10 ng ml−1 IL-17A for 4 h before injection."
NCBI gene IDs:
"crh11: 
crh12: "
Figure legend: "Candida SC5314 (a), TOR1-1/TOR1(b) and atg9Δ/atg9Δ (c) mutant strains were untreated (open column) or pre-incubated with IL-17A (filled column) for 4 h before exposure to neutrophils for additional 2 h before the assessment of adhesion, phagocytosis and killing. Error bars, mean±s.d. (n=3). The actual P-values are indicated (two-tailed t-test)."
NCBI gene IDs:
"TOR1-1: 
TOR1: 
atg9: 3635309"
Figure legend: "(h-j) Log10 colony-forming units (CFU) at 2 days post infection with C. albicans SC5314 (wild-type) or different mutant strains in C57BL/6 (h), IL-17F-deficient (i) or IL-17A-deficient (j) mice. Data are mean±s.d. (n=3). The actual P-values are indicated (two-tailed t-test)."
NCBI gene IDs:
"IL-17A: 16171
IL-17F: 257630"
Figure legend: "(m) ELISA assay showing failure of IL-17A to bind the Aspergillus mutant crf1Δ (blue square) as opposed to the wild-type strain AF293 (red square). Data are mean±s.d. (n =5). P-values ranged from 0.05 to 0.01, wild-type versus mutant strain exposed to 1 or 10 ng ml−1 IL-17A (one-way analysis of variance, Bonferroni post-test)."
NCBI gene IDs:
"crf1: "
Figure legend: "(n-q) IL-17A promotes filamentation in the mutant crf1Δ (o) but not the wild-type AF293 (q) strain after 24-h exposure in liquid culture. Scale bars, 500 μm."
NCBI gene IDs:
"crf1: "