EMBO/SourceData huggingface dataset:
Figure legend: "(D) Western blot of test translations using the (CGA-CCG) dicodon stalling mRNA reporter shown in (A). The mRNA reporter was added to a yeast in vitro translation extract obtained from a ski2∆uS10-3HA strain. Expression of the translation products (free His- and V5 tagged truncated uL4 protein and the same protein attached to tRNA) was visualized with an anti-V5 antibody."
NCBI gene IDs:
"uS10: 856371
ski2: 851114"
Figure legend: "(A) Western blot analysis after overexpression of Hel2 in strains expressing HA-tagged ribosomal proteins using an anti-HA antibody. Note the increase of the polyubiquitinated eS7 as well as uS10 and uS3."
NCBI gene IDs:
"Hel2: 851859"
Figure legend: "(C) Western blot analysis showing the role of Hel2 and Not4 in eS7A ubiquitination: Both copies of eS7 (eS7A and eS7B) were deleted and transformed with a plasmid containing HA-tagged eS7A (eS7-WT) or eS7A mutated in the four potential ubiquitination sites (4KR). Whole protein extracts we obtained from eS7AΔeS7BΔ cells or cells with an additional deletion in E3 ligases Hel2 and Not4 (hel2Δ and not4Δ). Note that the four mutated lysine residues were responsible for Not4-dependent monoubiquitination."
NCBI gene IDs:
"HA: 
hel2: 851859
Hel2: 851859
not4: 856799
Not4: 856799
eS7A: 854263
eS7: 854263
eS7B: 855628"
Figure legend: "(D) Western blot showing that Not4 was required for Hel2-mediated polyubiquitination of eS7 in Hel2-bound ribosomal complexes: Cells expressing HA-tagged eS7A or the eS7-4KR mutant, as well as PTH-tagged Hel2 in eS7AΔeS7BΔ and eS7AΔeS7BΔnot4Δ background were probed for eS7-ubiquitination. Either cell lysates or affinity-purified Hel2-ribosome complexes were used. The levels of the ubiquitinated eS7A in the lysates and in the affinity-purified samples with PTH-Hel2 were determined by Western blot analysis using an anti-HA antibody to detect eS7."
NCBI gene IDs:
"not4: 856799
Not4: 856799
eS7A: 854263
eS7: 854263///855628
eS7B: 855628"
Figure legend: "(E) Western blotting of in vitro ubiquitination assays of eS7A. The reactions were performed with the indicated components including His-tagged ubiquitin. Ribosomes were purified from Hel2Δ cells expressing HA-tagged eS7A. Polyubiquitinated HA-tagged eS7A (eS7A-3HA) was detected by Western blot analysis using an anti-HA antibody. We observed Hel2-mediated polyubiquitination of the monoubiquitinated eS7A."
NCBI gene IDs:
"Hel2: 851859
eS7A: 854263"
Figure legend: "(F) Western blot analysis of eS7A in vitro ubiquitination assays showing that Hel2-mediated polyubiquitination of eS7 was mainly K63-linked and required Not4-dependent monoubiquitination: These assays were performed similarly as described in (E) except yeast strains were lacking Not4 (not4Δ). Reactions were performed with the indicated components including His-tagged ubiquitin and several ubiquitin mutants and eS7A-ubiquitination was monitored using an anti-HA antibody."
NCBI gene IDs:
"not4: 856799
Not4: 856799"
Figure legend: "(A-C) Northern blots probing for the 5' NGD-IM in mutant cells expressing the R(CGN)12 reporter. (A) 5' NGD-IM detection in not4∆ski2∆, hel2∆ski2∆, slh1∆ski2∆, not4∆ slh1∆ski2∆. Quantification of full length and the 5'NGD-IM relative to the loading control (SCR1) is given below. Note the size difference of 5'-NGD IMs resulting from NGDRQC+ and NGDRQC- and that the shorter 5'-NGD intermediate (representing intermediates of the NGDRQC- pathway) was reduced in not4∆slh1∆ski2∆ mutant cells."
NCBI gene IDs:
"hel2: 851859
not4: 856799
SCR1: 9164887
ski2: 851114
slh1: 853187"
Figure legend: "Northern blots probing for the 5' NGD-IM in mutant cells expressing the R(CGN)12 reporter. (B) Northern blot as in (A) except uS10-WTski2∆ or uS10-K6/8Rski2∆ mutant cells with or without a deletion of Not4 (not4∆) were used. Note that the shorter 5'-NGD intermediates are also reduced in ski2∆not4∆uS10-K6/8R cells."
NCBI gene IDs:
"not4: 856799
Not4: 856799
uS10: 856371
ski2: 851114"
Figure legend: "Northern blots probing for the 5' NGD-IM in mutant cells expressing the R(CGN)12 reporter. (C) Cells expressing HA-tagged eS7A or the eS7A-4KR mutant in a ski2ΔeS7AΔeS7BΔ or ski2ΔeS7AΔeS7BΔslh1Δ backgrounds were probed for 5'-NGD intermediates by Northern blotting. Note that the four mutated lysine residues in eS7 were responsible for NGDRQC- in the R(CGN)12 reporter."
NCBI gene IDs:
"HA: 
eS7A: 854263
eS7: 854263
eS7B: 855628
ski2: 851114
slh1: 853187"
Figure legend: "(D) Western blot analysis showing that K83 and K84 of eS7A are main target sites for Hel2 and Not4-mediated ubiquitination: Similarly, as in Fig 6C, eS7AΔeS7BΔ mutant cells were used and wild type and single mutants of HA-tagged eS7A were expressed. Ubiquitinated eS7-HA was monitored using an anti-HA antibody."
NCBI gene IDs:
"eS7A: 854263
eS7B: 855628"
Figure legend: "(E) Northern Blot analysis probing for the levels of 5' NGD-IM derived from the R(CGN)12 reporter expressed in eS7AΔeS7BΔski2Δ cells and HA-tagged eS7 or eS7 mutants. We observed, that ubiquitination at K83 or K84 of eS7A is mainly responsible for NGDRQC-."
NCBI gene IDs:
"HA: 
eS7A: 854263
eS7: 854263
eS7B: 855628
ski2: 851114"
Figure legend: "(F) The levels of polyubiquitinated eS7A were increased by the overproduction of wild-type Hel2 (FL) but neither Hel2ΔRING nor Hel2(1-315) mutants. eS7A-3HAhel2Δ mutant cells harbouring the indicated plasmids expressing wild-type Hel2 (FL), Hel2ΔRING or Hel2(1-315) mutant proteins were harvested. Protein samples were analysed by Western blotting with an anti-HA (Top panel) or anti-FLAG antibody (Bottom panel)."
NCBI gene IDs:
"HA: 
Hel2: 851859
hel2: 851859
eS7A: 854263"
Figure legend: "F. RT-PCR analysis of RNA isolated from mouse liver for Birc5, FoxM1B and cyclin B1. Data are presented as log2 fold change and were calculated using non-operated mouse liver tissue as a control. Unpaired, two-tailed Student t-test was used to determine the significance between the log2 values of livers 6, 24 and 48 h post PH compared to the sham operated controls. Log2 values are plotted on a linear scale as mean ± SD."
NCBI gene IDs:
"Birc5: 11799
cyclin B1: 268697
FoxM1B: 14235"
Figure legend: "B. RT-PCR analysis of RNA isolated from mouse liver for FoxM1B, Birc5 and cyclin B1, Cyclin A2, Cyclin D1 and p21. RT-PCR data are presented as log2 fold change and each value post PH was calculated for each mouse by comparison to its resected liver lobe. Circle, with red outline marks the aged non-regenerating livers shown in Figure 2B. Each dot is representing an independent animal."
NCBI gene IDs:
"Birc5: 11799
Cyclin A2: 12428
cyclin B1: 268697
Cyclin D1: 12443
p21: 12575
FoxM1B: 14235"
Figure legend: "A. Eight weeks old mice were injected with liposomes coupled to scrambled siRNA (siScr) or sequences targeting MST1 & MST2 (siMST). Each dot is representing an independent animal. The % of Mst1 & Mst2 mRNA remaining in the liver was tested by RT-PCR, 1, 3 and 6 days post injection and calculated compared to control non-transfected livers. Unpaired, two-tailed Student t-test was used to calculate the significance of mRNA remaining in comparison to non-transfected control livers at each time point."
NCBI gene IDs:
"MST1: 15235
Mst1: 15235
MST2: 56274
Mst2: 56274"
Figure legend: "D. Quantitative RT-PCR analysis of RNA isolated from mouse liver for FoxM1B, Birc5 and cyclin B1, 1, 3 and 6 days post injection. Log2 fold change was calculated using non-transfected mouse liver as control. Unpaired two tailed Student t-test was used to calculate the significance of fold change of in comparison to a panel non-transfected control livers."
NCBI gene IDs:
"Birc5: 11799
cyclin B1: 268697
FoxM1B: 14235"
Figure legend: "D. Quantitative RT-PCR analysis of RNA isolated from mouse liver for FoxM1B, Birc5 and cyclin B1 at 0 and 40 h post PH. Log2 fold change was calculated using non-transfected/non-resected mouse liver as a control. Representative results of a single experiment with n = 6 animals per group are shown. Paired, two-tailed Student t-test was used to calculate the significant change in signals between liver tissues at the time of resection (0h) compared to 40 h post PH."
NCBI gene IDs:
"Birc5: 11799
cyclin B1: 268697
FoxM1B: 14235"
Figure legend: "Uncoupled (LEAK) mitochondrial respiration of soleus (SOL) and extensor digitorum longus (EDL) muscle of wild type (WT) vs. Ucp1-TG (TG) mice (WT n=9, TG n=5)."
NCBI gene IDs:
"Ucp1: 22227"
Figure legend: "Multi-tissue transcriptomic profiling of Gdf15 gene expression. Heatmap is shown as raw ct expression values (n=4 per genotype). Quantification of Gdf15 mRNA expression in TG mice is shown as fold change compared to WT littermates (WT n=5, TG n=4)."
NCBI gene IDs:
"Gdf15: 23886"
Figure legend: "Gdf15 gene expression of differentiated C2C12 muscle cells treated with vehicle control (Ctrl) or chemical mitochondrial uncoupler (FCCP, 1µM vs. 5µM) for 5hrs (n=3 biological replicates)"
NCBI gene IDs:
"Gdf15: 23886"
Figure legend: "Genotyping PCR panel of Gdf15 and HSA-Ucp1 loci shown for wild type (WT), Gdf15-KO (KO), Ucp1-TG (TG) and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Relative mRNA expression in quadriceps (Quad) of Ucp1 and Gdf15 in 20wks male WT (n=8), TG (n=7) and TGxKO (n=6) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Body mass (A), body lean mass (B) and body fat mass (C) development. Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Body mass (D), body lean mass (E) and body fat mass (F) during aging at 20wks, 45wks and 95wks of age. Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Subcutaneous white adipose tissue (sWAT) (G) and epididymal white adipose tissue (eWAT) (H) mass development at 10wks, 20wks, 45wks and 95wks of age. Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice at 10wks (WT n=6, TG n=7, TGxKO n=5), 20wks (WT n=10, TG n=10, TGxKO n=10), 45wks (WT n=5, TG n=6, TGxKO n=7) and 95wks (WT n=8, TG n=5, TGxKO n=5)."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Representative H&amp;E histological staining of sWAT at 20wks of age (scale bars represent 50 μm) Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "relative mRNA expression profile in sWAT of male mice (WT n=7, TG n=8, TGxKO n=8) (B). Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Representative immunoblots (C) and quantification (D) of UCP1 protein expression in sWAT of male mice at 20wks of age (WT n=4, TG n=6, TGxKO n=4). Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Relative mRNA expression profile in eWAT (WT n=7, TG n=8, TGxKO n=8). Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Plasma FGF21 levels from male mice at 20wks of age (n=10 per genotype). Plasma Leptin levels from male mice at 20wks of age (n=5 per genotype). Post-absorptive plasma insulin levels at 20wks of age (WT n=8, TG n=9, TG n=9). : Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Blood glucose (I) and insulin levels (J) with total area under the curve (AUC) of insulin (K) during oral glucose tolerance test (oGTT) at 17wks of age (WT n=8, TG n=11, TG n=11). Data are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice."
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Physical activity of male mice shown hourly over 24-hrs and as day and night time. Data shown are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice at 17-18wks of age (WT n=12, TG n=11, TG n=10) and shown as means + SEM"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "energy expenditure of male mice shown hourly over 24-hrs and as day and night time. Data shown are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice at 17-18wks of age (WT n=12, TG n=11, TG n=10) and shown as means + SEM"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: ", total assimilated energy of male mice shown hourly over 24-hrs and as day and night time. Table showing mean values of assimilated energy (kJ) at day/night time and per 24-hrs. Energy balance calculated as delta of assimilated energy (AE) and energy expenditure (EE) and energy balance (J) per 24-hrs. Data shown are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice at 17-18wks of age (WT n=12, TG n=11, TG n=10) and shown as means + SEM"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Respiratory quotient (RQ) shown hourly over 24-hrs (J), RQ amplitude (K) and metabolic flexibility via percentage relative cumulative frequency (PRCF) (L). Data shown are from male wild type (WT), Ucp1-TG (TG), and Ucp1-TGxGdf15-KO (TGxKO) mice at 17-18wks of age (WT n=12, TG n=11, TG n=10) and shown as means + SEM"
NCBI gene IDs:
"Gdf15: 23886
Ucp1: 22227"
Figure legend: "Relative quadriceps (Quad) mRNA expression of the ISR components Atf4, Atf5, Atf6 and Chop (WT day n=9, WT night n=5, TG day n=20, TG night n=12). Data shown are from male wild type (WT) vs. Ucp1-TG (TG) mice sacrificed at day (10am) or night (10pm)."
NCBI gene IDs:
"Atf4: 11911
Atf5: 107503
Atf6: 226641
Chop: 13198
Ucp1: 22227"
Figure legend: "Representative immunoblots of ISR component eIF2α (B) and quantification of phospho-eIF2α (p-eIF2aSer51) relative protein expression (C) in skeletal muscle (Gastroc, WT day n=4, WT night n=5, TG day n=9, TG night n=9). Data shown are from male wild type (WT) vs. Ucp1-TG (TG) mice sacrificed at day (10am) or night (10pm)."
NCBI gene IDs:
"Ucp1: 22227"
Figure legend: "Relative mRNA expression of Rev-erba (D) and Gdf15 (E) in quadriceps, liver and eWAT (WT day n=9, WT night n=5, TG day n=20, TG night n=12). Data shown are from male wild type (WT) vs. Ucp1-TG (TG) mice sacrificed at day (10am) or night (10pm)."
NCBI gene IDs:
"Gdf15: 23886
Rev-erba: 217166
Ucp1: 22227"
Figure legend: "Skeletal muscle (Quad) GDF15 protein content normalized to total protein content (WT day n=4, WT night n=5, TG day n=14, TG night n=12) Circulating GDF15 plasma levels (WT day n=9, WT night n=5, TG day n=18, TG night n=12). Data shown are from male wild type (WT) vs. Ucp1-TG (TG) mice sacrificed at day (10am) or night (10pm)."
NCBI gene IDs:
"Ucp1: 22227"
Figure legend: "(e, f) (e) RIP qPCR and (f) PCR analysis performed using an antibody against endogenous ARID1B. for monitoring NEAT1_2 levels. RIP with NONO and IgG was used as a positive and negative control respectively. GAPDH levels were also assessed in the assay. Data are expressed as a percent of Input."
NCBI gene IDs:
"GAPDH: 2597
NEAT1: 283131"
Figure legend: "(c) smFISH analysis was performed to detect NEAT1 signal in the nucleus. The scale bar corresponds to 10 µm. Graph showing the FISH signal quantification across the knockdowns in comparison to scramble control. The number of nuclei (n) used for quantification is mentioned."
NCBI gene IDs:
"NEAT1: 283131"
Figure legend: "A)  Cyclin B2 knock-out and induced degradation by immunoblotting. Indicated cell lines were analysed 24 hours after mock or Dox/IAA/Asv (DIA) treatment using the indicated antibodies to confirm homozygous gene tagging and efficiency of  protein degradation. ( "
NCBI gene IDs:
"Cyclin B2: 9133"
Figure legend: "(D) Cell proliferation of A2dd, CycB1dd and B1dd/B2ko following mock or DIA treatment. 1000 cells were plated in each well (diameter 3.5cm) and incubated for 10 days before methanol fixation and Crystal Violet staining."
NCBI gene IDs:
"A2: 890
B1: 891
CycB1: 891
B2: 9133"
Figure legend: "(E) Kinetics  of mitotic entry as measured by time lapse microscopy in A2dd and B1dd/B2ko cells following mock or four-hour DIA treatment of asynchronous cells. The cells were imaged for 16h with 5min intervals using widefield  DIC, mitotic entry was manually scored by detecting cell rounding. Curves display the cumulative mitotic index (data from three repeats, n&gt;500 cells per condition, s. d. indicated by shaded area)."
NCBI gene IDs:
"A2: 890
B1: 891
B2: 9133"
Figure legend: "(I) Cyclin A2  siRNA depletion causes endoreplication. Following 72 hours of siRNA transfection MCF7 and MCF10A cells were analysed by  PI staining and  FACS. The histograms show the changes in  DNA  content (PI Int.) towards &gt;4N following cyclin A2 depletion."
NCBI gene IDs:
"Cyclin A2: 890
cyclin A2: 890"
Figure legend: "(J) Cyclin A2 siRNA and degron depletion in RPE-1  cells. RPE-1 OsTIR1  and RPE-1 A2dd cells were subjected to 72 hours of cyclin A2 siRNA depletion and/or of DIA treatment as indicated and probed for cyclin A2 levels by  immunoblotting. The longer exposure (L.E.) reveals incomplete depletion of cyclin 2  by siRNA."
NCBI gene IDs:
"TIR1: 
A2: 890
Cyclin A2: 890
cyclin A2: 890"
Figure legend: "(C) Representative images from  live-cell imaging of SiR-Tubulin labelled (red) mitotic B1dd/B2ko cells expressing FusionRed-H2B (green), Mis12-GFP (white); time in mins, scale bar = 5µm. Cells were imaged four hours after DIA treatment."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(G) Upper panel, confirmation of  Cdc27  depletion by  immunoblotting 72 hours after siRNA transfection in  B1dd/B2ko cells. Lower panel, following 40-hour siRNA transfection B1dd/B2ko cells were treated for 24 hours with Thymidine, released for 10 hours and treated with proTAME and Apcin for additional four hours. At this point, the cells were fixed and  stained with tubulin and pericentrin for  immunofluorescence  analysis."
NCBI gene IDs:
"B1: 891
B2: 9133
Cdc27: 996"
Figure legend: "(H) Representative images of mitotic spindles in control- and DIA-treated B1dd/B2ko cells following arrest in mitosis by proTAME/Apcin treatment Tubulin staining  is shown in white, pericentrin in green and  DAPI in red, scale bar = 5µm."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(L) Immunofluorescence  images from mitotic Ctr and DIA treated  B1dd/B2ko cells after P/A synchronisation stained with anti-alpha-tubulin (green), anti-gamma-tubulin (red) antibodies and DAPI  (blue), scale bar = 5µm. Cells were exposed to ice-cold medium and either fixed immediately after cold exposure, or incubated for 5,10, or 20 minutes at 37° C before fixation."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(A) Time-lapse  images of PCNA-mRuby tagged  A2dd cells. The imaging sequence was started at the time of Doxycycline addition, and degron activity was triggered three hours later by addition of  IAA  and Asv or PBS (indicated by dashed line). Time is shown as hh:min, scale bar equals 10µm. For further analysis cells were chosen that had dissipated their PCNA foci before the addition of DIA to ensure that cyclin A2 degradation was triggered in G2 phase."
NCBI gene IDs:
"A2: 890
cyclin A2: 890"
Figure legend: "(D) Images from time-lapse sequence of mitosis showing cell division  in controls and after addition of 0.5µM PD-166285 in DIA treated  A2dd labelled with SiR-DNA (time  is indicated as hh:min, the scale bars represent 20µm)."
NCBI gene IDs:
"A2: 890"
Figure legend: "(D) Cyclin A2dd cells with inducible CycB1-YFP (B1-WT) and  CycB1-YFP-NLS (B1-NLS) were analysed for DIA induced cyclin B1  expression / cyclin A2 depletion. Samples were collected at indicated time points and probed by immunoblotting with cyclin B1  and cyclin A2 antibodies"
NCBI gene IDs:
"YFP: 
Cyclin A2: 890
CycB1: 891"
Figure legend: "(I) Immunoprecipitation of YFP from extract from   A2dd following induction of YFP (Y), CycB1-YFP (BY) and CycB1-YFP-NLS (BYN). The samples in the odd lanes (IAA/Asv-) were treated with 1µg/ml  doxycycline alone, while samples in even lanes (IAA/Asv+) were treated with Auxin and  Asv to induce cyclin A2 depletion. Total cell extract and  immune-precipitates  were probed by immunoblotting with the indicated antisera."
NCBI gene IDs:
"A2: 890"
Figure legend: "(F) Representative images of  Ki-67 immunofluorescent staining of chromosome  spreads from control (Ctr) and DIA treated P/A synchronised  B1dd/B2ko cells and quantification of Ki-67 staining  intensity using cross-sections of chromosomes"
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(G) Representative images from  live-cell imaging  of DIA-treated SiR-Tubulin labelled (red) B1dd/B2ko cells expressing FusionRed-H2B (green) and AurB-GFP  (white); time is indicated in minutes, scale bar = 5µm. Bottom panel shows frequencies of aberrant AurB localisation in P/A synchronised B1dd/B2ko cells."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(J) Tpx2 shows reduced spread on mitotic chromosomes in DIA treated B1dd/B2ko cells. The top panel shows immunofluorescence images, for quantification we measured the mean intensity of Tpx2 staining on the centrosomes and spindle and plotted the ratio of these values per cell. We also analysed the centrosome/spindle distribution of  AurA  that does not change following DIA treatment."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(B) Confirmation of ENSA/ARPP19 S67 phosphorylation in mitotic extracts from Ctr and DIA treated cells, before and after ENSA/ARPP19 siRNA depletion."
NCBI gene IDs:
"ARPP19: 10776
ENSA: 2029"
Figure legend: "A) Representative images from live-cell imaging of siRNA transfected B1dd/B2ko cells (FusionRed-H2B in red, SiR-Tubulin  in white, scale bar = 10µm). Bar-plot panels on the right show single-cell analysis of 10 cells manually scored for length mitosis pre-NEBD and post-NEBD. Entry into  prophase was scored by cell rounding and NEBD was identified by influx of Tubulin in the  nucleus."
NCBI gene IDs:
"B1: 891
B2: 9133"
Figure legend: "(B) Widefield Imaging. DIC (Grey), SiR-DNA  (b/w), of ENSA/ARPP19 siRNA transfected DIA treated B1dd/B2ko cells. Time is indicated in hh:min, scale bar = 10µm."
NCBI gene IDs:
"ARPP19: 10776
B1: 891
B2: 9133
ENSA: 2029"
Figure legend: "(A) Mettl14 cKO leads to perinatal lethality. Newborn cKO pups are smaller with tight and shiny skin."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(C) H/E staining of newborn skin sections from WT and Mettl14 cKO mice."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(A)  EdU staining of WT or Mettl14 cKO skin  after pulse-chase labeling. Skin samples were counterstained with antibody against β4-integrin. Note reduced EdU label-retaining cells in cKO  skin epidermis. Arrows indicate  Edu-positive cells. (B) Label-retaining cells in WT or Mettl14 cKO skin were quantified and shown as box plots. The plot indicates the mean (solid diamond within the box), 25th percentile (bottom line of the box), median (middle line of the box), 75th percentile (top line of the box), 5th and 95th percentile (whiskers), 1st and 99th percentile (solid triangles) and minimum and maximum measurements (solid squares). n=6 (biological repeats), P&lt;0.05 (Student's t-test). n=19, P&lt;0.01 (Student's t-test). "
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(C) Number of holoclones derived from WT and Mettl14 cKO skin was quantified and shown as box and whisker plots. The plot indicates the mean (solid diamond within the box), 25th percentile (bottom line of the box), median (middle line of the box), 75th percentile (top line of the box), 5th and 95th percentile (whiskers), 1st and 99th percentile (solid triangles) and minimum and maximum measurements (solid squares). n=6 (biological repeats), P&lt;0.05 (Student's t-test). n=24, P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(D) Morphology  of primary keratinocytes isolated from WT or Mettl14 cKO skin."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(F) Proliferation of WT and Mettl14  null cells in vitro was quantified and shown as dot plots. n=3, P&lt;0.01 (Student's t-test) for Day 6, 9, and 12, and P&lt;0.05 (Student's t-test) for  Day 3. Error bar represents S.D."
NCBI gene IDs:
"Mettl14: 210529"