EMBO/SourceData huggingface dataset:
Figure legend: "HeLa cells were depleted of Bub1 using RNAi Immunofluorescence images were shown for Bub1 and ZW10 localization. Quantification of Bub1 and ZW10 kinetochore levels normalized to CREST in the indicated conditions. with the indicated Bub1 fusion proteins"
NCBI gene IDs:
"Bub1: 699"
Figure legend: "(A) Cell proliferation for PC3 cells transfected with siSCR or siCLU monitored in real time for 60h with SP Dynamic Xcelligence system (Voltage 2.5 K). Error bars represent mean ± SEM, n=4. Slopes compared by ANCOVA test, p&lt;0.0001."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(B) Left panel: Cell cycle profile of PC3 cells transfected with siSCR or siCLU. Right panel: Percentage quantification of variation in G1, S and G2/M phases. Error bars represent mean ± SEM, n=3, **p&lt;0.01 by paired Student's t-test. (G2/M population p= 0.0058; G1 p=0.008.). Inset: Western blot with CLU antibody in PC3 cells after CLU silencing. Vinculin was used as loading control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(C) Left panel: RNA-Microarray analysis comparing PC3 cells transfected with siCLU versus siSCR and Kinexus phosphokinome microarray analysis comparing LNCaP-transfected with siCLU versus siSCR. Target genes expression is shown as fold change relative to siSCR, p ≤0.05 by an unpaired t-test for all the genes listed. Central panel: Venn diagram to graphically illustrate the overlap of mRNA and proteins involved in the regulation of mitosis in both transcriptome and phosphokinome analysis. The comparison of these 2 experiments shows modification of 4 mitosis regulators. Right panel: Western blot validating the expression levels of target proteins."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(A) Real-time PCR for CLU and Cdc25C in three different prostate cancer cell lines. Target genes expression was calculated relative to GAPDH and normalized to siSCR. Error bars represent mean ± SD, n=3."
NCBI gene IDs:
"GAPDH: 
Cdc25C: 995
CLU: 1191"
Figure legend: "(B) Western blot for CLU and Cdc25C after siSCR or si CLU transfection in the same prostate cancer cell lines as in A. Vinculin was used as loading control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(C) Western blot for CLU and Cdc25C in PC3 cells after transfection with siSCR or siCLU followed by transfection with CLU or empty expression plasmid for additional 48h. Vinculin was used as loading control"
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(B) Western blot with the correspondent quantification of protein levels (left) and real time PCR (right) from untreated LNCaP xenografts harvested at endpoint. The negative correlation between CLU-RNA and Cdc25C-RNA ΔΔCT values was calculated using a spearman test (rho=-0.86; p=0.0107)."
NCBI gene IDs:
"Cdc25C: 995
CLU: 1191"
Figure legend: "(C) Duolink proximity ligation assay between Cdc25C and CLU in PC3 cells. Confocal microscopy was used to detect the interaction (red dots). DNA was counterstained with DAPI (blue). PC3 cells transfected with siCLU were used as a negative control. Scale bar represent 10 µm."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(A) Left panel: Western blot for CLU, Cdc25C, Cdc25C-T48, Cdc25C S216 and Cdc25C S198 in PC-3 cells after siSCR or siCLU transfection, synchronization and nocodazole release at indicated time points. Vinculin was used as loading control. Right panel: Immunofluorescence microscopy for Cdc25C-T48 in PC3 cells after siSCR or siCLU transfection. DNA was counterstained with (DAPI) (blue). Scale bar represents 10 µm."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(B) Western blot for CLU, Cdc25C-T48, Cdk1 and PP2A in PC3 cells after siSCR or siCLU transfection and after synchronization followed by treatment with or without okadaic acid (OA) (100 nM) for 12 hours. Proteins were extracted 15 min after nocodazole release. Vinculin was used as loading control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(D) PP2A phosphatase activity measurement in PC3 cells after siSCR or siCLU transfection, synchronization and nocodazole release at indicated time points. Error bar represent mean ± SEM, n=3, ** p&lt;0.01 (p=0.0025) by ANOVA followed by Bonferroni's post hoc comparisons. Cells treated with no peptide were used as a negative control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(A) Western blot for CLU, Cdk1 Y15, Cdk1 and Wee1 in PC-3 cells after siSCR or siCLU transfection followed by synchronization and nocodazole release at indicated time points. Vinculin was used as loading control"
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(B) Wee1 expression level was evaluated in PC3 cells after CLU silencing by real-time PCR. Error bar represent mean ± SEM, n=3, *** p&lt;0.001 (p=0.0001) by unpaired t-test followed by Welch's correction."
NCBI gene IDs:
"CLU: 1191
Wee1: 7465"
Figure legend: "(C) FACS analysis showing percentage of the PC3 cells positive for phosphohistone H3 after transfection with siSCR or siCLU in presence and absence of Wee1 inhibitor, MK-1775. Error bar represent mean ± SEM, n=3, ****p&lt;0.0001 by Mann-Whitney"
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(D) Western blot for CLU, Wee1, Cdk1 Y15, Cdk1 and Cyclin B1 in PC3 cells after transfection with siSCR or siCLU followed by synchronization and treatment with or without MK-1775, a specific Wee1 inhibitor, for 12 hours. Cells were harvested at different time points after nocodazole release. Vinculin was used as loading control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(A) Cabazitaxel IC50 calculation using WST1 assay in PC3 cells after transfection with siSCR or siCLU (left), and SCR or OGX-011 (right) followed by 72h of cabazitaxel treatment. Percentage of surviving cells was calculated relative to control. Error bar represent mean ± SEM, n=3. Inset: Western blot was performed to verify CLU knockdown in cells."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(B) Western blot for CLU, Cdc25C, Cdc25C-T48, Cdk1, Cdk1-Y15 and Wee1 in IGRCaP-1 and PC3 parental (WT) or cabazitaxel-resistant (R-caba) cells as well as for PC3-R-caba cells transfected with siSCR or siCLU. Vinculin was used as loading control."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "(D) Cell survival measured using WST1 in PC3 resistant cells after transfection with siSCR or siCLU followed by treatment with cabazitaxel (10 nM; C10), MK-1775 (10 nM; MK10) or both for 72 hours. .Error bar represent the mean ± SEM, n=3, *** p&lt;0.001 by one way ANOVA followed by Bonferroni's post hoc correction."
NCBI gene IDs:
"CLU: 1191"
Figure legend: "A. Ovaries from control heterozygous Rpp3018.2 flies (left), homozygous Rpp3018.2 or transheterozygous Rpp3018.2/Rpp30PE flies (middle) and homozygous Rpp3018.2 carrying a copy of ubiRpp30GFP transgene (right). Scale bar, 100µm."
NCBI gene IDs:
"GFP: 
Rpp30: 44392"
Figure legend: "E. Rpp30-dependent early oogenesis arrest is restored by expression of Rpp30GFP transgene in two different genetic backgrounds: Left. Homozygous Rpp3018.2 ovariole expressing ubiquitous ubiRpp30GFP (st, stage). Right. Transheterozygous Rpp3018.2/Rpp30PE ovarioles overexpressing germline specific nanosGal4,UASRpp30GFP. Note follicular somatic cells with no Rpp30GFP expression (arrowhead). Magnifications show nuclear Rpp30 localization, Rpp30 perinuclear foci (arrows), and Orb (a specific oocyte marker). Scale bar, 10µm."
NCBI gene IDs:
"Gal4,UAS: 
Rpp30: 44392"
Figure legend: "F. flp/FRT clones mutant for Rpp3018.2specifically in the germline (star) and/or the follicular cells (arrows) are detected by the absence of GFP. Magnifications show mutant oocytes with Orb mislocalization next to the karyosome (dotted circle) instead of being localized to the posterior, as seen in stage 3 (st.3) wild-type clones. Scale bar, 10µm."
NCBI gene IDs:
"Rpp30: 44392"
Figure legend: "Figure 2. Rpp30 is required for pre-tRNA processing.Whole fly RNA extracts were used to study tRNA processing by Northern Blot. Top box : the three different probes used are depicted : 5' and 3' probes (red and green, respectively) can detect full pre-tRNAs and tRNAs intermediates, but will not detect mature tRNAs. An Internal Probe (IP, blue) corresponding to the anti-codon region was used to detect all non-mature and mature tRNAs. The genotypes used in this experiment are numbered at the bottom (1-6). Northern Blot panels: tRNA-his 5' probe (top), tRNA-his 3' probe (middle) and tRNA-his IP (bottom). U6 probe was used as loading control."
NCBI gene IDs:
"U6: 
Rpp30: 44392"
Figure legend: "A. Rpp3018.2 homozygous early oogenesis arrest is partially rescued by p53 mutation. Scale bar,100μm."
NCBI gene IDs:
"p53: 2768677
Rpp30: 44392"
Figure legend: "B. Early oogenesis arrest found in Rpp3018.2 homozygous ovaries is rescued by chk2 mutation (Rpp3018.2 , mnkp6 and Rpp3018.2/ Rpp30PE, mnkp6). Scale bar, 100μm. Star: example of one rescued stage 9 egg chamber. Arrow: example of non-rescued egg chambers."
NCBI gene IDs:
"chk2: 35288
Rpp30: 44392"
Figure legend: "C. Germline clones mutant for Rpp3018.2 were immunostained for PCNA (red). DAPI is in blue. Magnifications: PCNA signal in a control or mutant nurse cell. Scale bar, 10μm."
NCBI gene IDs:
"Rpp30: 44392"
Figure legend: "D. Germline clones mutant for Rpp3018.2 were immunostained for Brf (pol-III) (red). DAPI is in blue. A Z-projection of Brf staining is shown. The arrow points Brf aggregates in a mutant chamber. Scale bar, 10μm."
NCBI gene IDs:
"Rpp30: 44392"
Figure legend: "E. Early oogenesis arrest found in Rpp3018.2 homozygous ovaries is partially rescued by claspin mutation (Rpp3018.2; claspinEP/TM3). Scale bar, 100μm."
NCBI gene IDs:
"claspin: 326205
Rpp30: 44392"
Figure legend: "G. Top panel. Early oogenesis arrest found in Rpp30182./PEtransheterozygous ovaries is rescued by sqd-S-HAoverexpression. Scale bars: 100µm. Low panel. Rpp3018.2/PE ; sqd-S-HA/sqd-S-HA ovaries were dissected and stained for Orb (green), HA (squid, red) and DAPI (blue). Scale bar, 10µm."
NCBI gene IDs:
"Rpp30: 44392
sqd: 41666"
Figure legend: ". D. Nuage specific markers (Aub, Ago3 and Mael) are shown in stage 3 wild-type and germline mutant clones for Rpp3018.2. Scale bar, 10µm."
NCBI gene IDs:
"Rpp30: 44392"
Figure legend: "E-H. Double mutant ovaries for Rpp30 and chk2 (Rpp3018.2, mnkp6) rescued the specific and general piRNA populations, nuage morphology and the characteristic ping-pong signal. Scale bar, 10µm."
NCBI gene IDs:
"chk2: 35288
Rpp30: 44392"
Figure legend: "D. Testes from control heterozygous, transheterozygous Rpp3018.2/Rpp30PE or aubHN/QC42 were fixed and stained for Stellate crystal formation. Z-projections are shown."
NCBI gene IDs:
"aub: 34524
Rpp30: 44392"
Figure legend: "  Quantification of mRNAs in ASPC-1 cells untreated or treated with OMP 80 µg/ml, 5-FU 5 µg/ml or the combination of both was performed at different time points throughout 24 hours, and the means of three replicates are shown for every time point. The stars indicate significant differences after 24 hours compared to control. The pro-apoptotic bad-mRNA was upregulated after 5-FU- and 5-FU+OMP-treatment. The antiapoptotic bcl-2 mRNA was downregulated by 5-FU and bcl-XL by 5-FU+OMP. OMP led to downregulation of bad and survivin and to upregulation of the mdr-1mRNA.  "
NCBI gene IDs:
"mdr-1: 5243
bad: 572
bcl-2: 596
bcl-XL: 598
survivin: 332"
Figure legend: "  mRNA quantification in MiaPaCa-2 cells untreated or treated with OMP 80 µg/ml, 5-FU 5 µg/ml or the combination of both was performed at different time points throughout 24 hours, and the means of three replicates are shown for every time point. While the mdr-1 mRNA is not signicantly changed, the vATPase mRNA is upregulated in the 5-FU+OMP group after 24 hours compared to control.  "
NCBI gene IDs:
"vATPase: 
mdr-1: 5243"
Figure legend: "E Levels of LMs and PGE2 released by macrophages in presence of CVF, CLys and CSN Additionally, macrophages were lysed and gene expression of PTGS2, PTGES, ALOX5, ALOX15 and MaR1 quantified by RT-PCR."
NCBI gene IDs:
"ALOX15: 246
ALOX5: 240
PTGES: 9536
PTGS2: 5743
MaR1: 388015"
Figure legend: "(E) ASS1 expression from the MILE study. The dotted line revers for average of Healthy Bone Marrow. Data information: For statistical analysis, an ordinary one-way ANOVA with Dunnett's correction for multiple comparisons was performed a Kruskal-Wallis test was performed on E with **** referring to P&lt;.0001 in E."
NCBI gene IDs:
"ASS1: 445"
Figure legend: "(B) Immunogold electron micrograph of GFP  or ORF6-GFP  expressed HeLa  cells. Blue arrows mark ORF6-GFP  dots are enriched on the surface of LDs. Green arrows mark GFP  dots are distributed in the cytoplasm. Scale bar represents 500 nm."
NCBI gene IDs:
"GFP: 
ORF6: 43740572"
Figure legend: "(C, D and E) HeLa  cells stable expressing ORF6-Flag  were treated with or without 200 μM OA  for 12 h, then fixed and stained  with anti-Flag  (red). Initial LDs  were labeled with GFP-GPAT4152-208 (green), mature LDs  were labeled with BODIPY-493/503 (green) or GFP-Plin2  (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(B) Analysis of the homodimerization  of ORF6  by cross-linking with DSS. HEK293T  cells expressing ORF6-Flag  were treated with 0, 0.1, 0.2 and 0.3 mM DSS  for 30 min. Cell lysates were analyzed via WB."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(D) The indicated Flag  tagged ORF6  or its mutants were co-expressed in HEK293T  cells with HA  tagged ORF6. Protein interactions   were analyzed by immunoprecipitation  with anti-Flag  beads and immunoblotting  analysis."
NCBI gene IDs:
"Flag: 
HA: 
ORF6: 43740572"
Figure legend: "(F) HEK293T  cells were transfected with ORF6-Flag  or ΔNTD-Flag  or ΔAH1-Flag  or ΔAH2-Flag  and were treated with 0.1 mM DSS  for 30 min. Cell lysates were analyzed via WB."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(G) HeLa  cells were transfected with ORF6-Flag  or indicated mutants and were treated with 200 μM OA  for 12 h, and then fixed and stained  with anti-Flag  (red). LDs  were labeled with BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(A) HeLa  cells were transfected with vector or ORF6-Flag  for 24 h, and then treated with 200 μM OA  for indicated times. Cells were fixed and stained  with anti-Flag  (green). LDs  were labeled with LipidTox Deep Red  (red). The nuclei  were stained  with DAPI. Cells were imaged by  confocal microscopy. Scale bar represents 10 μm. White ROIs indicate cells expressing ORF6  and yellow ROIs indicate the cells without ORF6 expression."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(D) HeLa  cells stable expressing vector or ORF6-Flag  were treated or untreated with 50 μM ATGL  inhibitor (ATGLi) for 24 h, and then were fixed and stained  with anti-Flag  (red). LDs  were labeled with  BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm. (E) Mean number of LDs  in each cell in (D) was counted from 25 cells of three independent experiments. Two-tailed Unpaired Student's t-test, ***p &lt; 0.001, ****p &lt; 0.0001. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(H) HeLa  cells stable expressing vector or ORF6-Flag  were treated or untreated with 50 μM ATGL  inhibitor, along with DMSO  or 1 μM DGAT1  inhibitor (DGAT1i) or/and 2 μM DGAT2  inhibitor (DGAT2i) for 24 h. LDs  were labeled with BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm. (I) Mean number of LDs in each cell in (H) was counted from 35 cells of three independent experiments. Two-tailed Unpaired Student's t-test, ***p &lt; 0.001, ****p &lt; 0.0001, ns means no significance. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(A) Subcellular fractions were isolated from GFP  or ORF6-GFP  over-expressed cells. Calnexin  represent the ER, Plin2  represent LDs, Rab11  represent endosomes, VDAC  represent mitochondria, and Tubulin  represents cytoplasm, respectively."
NCBI gene IDs:
"GFP: 
ORF6: 43740572"
Figure legend: "(E) ORF6-Flag  or the mutants were co-expressed with mCheey-RAMP4. Cells were treated with 200 μM OA  for 12 h, and then fixed and stained  with anti-Flag  (red). The ER  was visualized with mCheey-RAMP4  (red). LDs  were labeled with LipidTOX Deep Red (white). Cells were imaged by confocal microscopy. Scale bar represents 10 μm."
NCBI gene IDs:
"Flag: 
mCheey: 
ORF6: 43740572
RAMP4: 27230"
Figure legend: "(J) HeLa  cells stable expressing ORF6-Flag  were transfected with si-BAP31  and/or si-USE1  for 24 h, and were further treated with 50 μM ATGL  inhibitor for 24h. Cells were fixed and stained  with anti-Flag  (red). LDs  were labeled with BODIPY-493/503 (green). The nuclei  were stained  with DAPI. Cells were imaged by  confocal microscopy. Scale bar represents 10 μm. (K) Mean number of LDs  in each cell in (J) was counted from 25 cells of three independent experiments. Two-tailed Unpaired Student's t-test, *p &lt; 0.05, **p &lt; 0.01. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
BAP31: 10134
ORF6: 43740572
USE1: 55850"
Figure legend: "(L)  SARS-CoV-2-infected  Vero-E6  cells were transfected with control, or si-BAP31  and/or si-USE1  for 48 h. Cell lysates were analyzed via WB. Supernatants were determined by  plaque assays. Virus titer values represent mean ± SD for three independent replicates. Two-tailed Unpaired Student's t-test, ****p &lt; 0.0001, ns means no significance. Error bars represent the mean ± SD."
NCBI gene IDs:
"BAP31: 103232989
USE1: 103234122"
Figure legend: "(A) HeLa  cells were transfected with vector or ORF6-Flag  for 12 h and treated with 200 μM OA  for another 12 h, the medium was removed and replaced with fresh complete culture containing 1 μM DGAT1  inhibitor and 2 μM DGAT2  inhibitor for 24 h. Meanwhile, 50 μM ATGL  inhibitor or 100 μM Chloroquine  (CQ) was added and allowed to incubate for 24 h or 4 h to block lipolysis  or lipophagy, respectively. Cells were fixed and stained  with anti-Flag  (red). LDs  were labeled with BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm. White ROIs indicate cells expressing ORF6  and yellow ROIs indicate the cells without ORF6 expression."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(C) HEK293T  cells were transfected with ORF6-Flag. Protein interactions  were detected by immunoprecipitation  with anti-Flag  beads and immunoblotting  analysis with ATGL, HSL, and CGI58 antibodies."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(H and I) The effect of ORF6-Flag  or the ΔNTD mutant on the interactions  of Flag-ATGL  with GFP-Plin2  or GFP-UBXD8  was analyzed by immunoprecipitation."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(K and L)  Vero-E6  cells were transfected with Flag-ATGL  and GFP-Plin2  or GFP-UBXD8  for 24 h and infected or non-infected with SARS-CoV-2 for 24 h. Protein interactions  were analyzed by immunoprecipitation  with anti-GFP beads and immunoblotting  analysis."
NCBI gene IDs:
"ATGL: 
Flag: 
GFP: 
UBXD8: 23197
Plin2: 123"
Figure legend: "(C) Representative transmission electron micrograph  of ORF6-Flag  stable expressing or vector HeLa  cells upon OA  treatment. Red arrows mark the contact sites between LDs  and mitochondria. M, mitochondria. LD,  lipid droplets. Scale bar represents 1 μm. (D) The number of mitochondria-LD  contacts per cell was counted from 20 cells. The number of mitochondrial  engaged in mitochondrial-LD  contact per LD  in (C) was counted from 20 LDs. Two independent experiments. Two-tailed Unpaired Student's t-test, ****p &lt; 0.0001. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(H) Cos7  cells expressing ORF6-Flag  and vector were treated with 200 μM OA  for 12 h, and then were fixed and stained  with anti-Flag  (white) and anti-Tom20  (red). Tom20  represents mitochondria  marker. LDs  were labeled with BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(L) Seahorse FAO assays  to examine the oxidation of endogenous FAs  in vector, or ORF6-Flag  or ORF64Q-Flag  over-expressed HeLa  cells. Three biological replicates were performed with similar results. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
ORF6: 43740572"
Figure legend: "(N) Vero-E6 cells stable expressing GFP  or GFP-Plin5  or GFP-Plin5Δ443-463 were infected with SARS-CoV-2 for 24 h. Cell viability was analyzed. Cell lysates were analyzed via WB. Supernatants were determined by plaque assays. Virus titer  values represent mean ± SD for three independent replicates. Two-tailed Unpaired Student's t-test, **p &lt; 0.01, ns means no significance. Error bars represent the mean ± SD."
NCBI gene IDs:
"GFP: 
Plin5: 440503"
Figure legend: "(C) HeLa  cells were transfected with negative, or si-MTX1, or si-MTX2, or si-SAMM50  for 24 h and further transfected with ORF6-GFP  24 h. Subcellular fractions were isolated from cells. VDAC1  and ATP5A1  represent mitochondria  markers, Tubulin  represents cytosolic  marker."
NCBI gene IDs:
"GFP: 
MTX1: 4580
MTX2: 10651
ORF6: 43740572
SAMM50: 25813"
Figure legend: "(D) HeLa  cells were transfected with negative or si-MTX1  and si-MTX2 for 24 h and further transfected with ORF6-GFP  24 h. Subcellular fractions were isolated from cells. VDAC1  and ATP5A1  represent mitochondria  markers, Tubulin  represents cytosolic  marker."
NCBI gene IDs:
"GFP: 
MTX1: 4580
MTX2: 10651
ORF6: 43740572"
Figure legend: "(F) Cos7  cells expressing ORF6-Flag  were transfected with negative or si-MTX1/si-MTX2/si-SAMM50  (Triple KD) for 36 h and treated with 200 μM OA  for another 12 h. Cells were fixed and stained  with anti-Flag  (white) and anti-Tom20  (red). Tom20  represents mitochondria  marker. LDs  were labeled with BODIPY-493/503 (green). Cells were imaged by confocal microscopy. Scale bar represents 10 μm. (G) Quantification of average number of LD-mitochondria  contacts per LD  from (F). 25 cells (Negative), 50 cells (Triple KD) from three independent experiments were calculated. Two-tailed Unpaired Student's t-test, ****p &lt; 0.0001. Error bars represent the mean ± SD."
NCBI gene IDs:
"Flag: 
MTX1: 4580
MTX2: 10651
ORF6: 43740572
SAMM50: 25813"
Figure legend: "HeLa cells overexpressing the HA-tagged MICU1 WT, MICU1 S124D or MICU1 S124A mutants were stained for HA or HSP60 (mitochondrial marker). Merged images are indicated (merge). Scale bar 10 μm"
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Representative images of the 2mt-GCaMP6m 474/410 ratio of ShMICU1 HeLa stable cells expressing an empty vector (ctrl) or the MICU1 WT, MICU1 SD, and MICU1 SA. Scale bar 10 μm"
NCBI gene IDs:
"MICU1: 10367
MICU1: 216001"
Figure legend: "Resting mitochondrial calcium levels, evaluated through ratiometric imaging of the mitochondrial targeted GCaMP6m, in ShRNA control (plko) or ShRNA MICU1 HeLa stable clone cells transfected with the indicated constructs (n=5 independent experiments; 55-67cells)"
NCBI gene IDs:
"plko: 
MICU1: 10367"
Figure legend: "Representative kinetics (E) and analysis (F) of aequorin-based [Ca2+]m measurements in ShRNA MICU1 HeLa stable clone cells transfected with the indicated constructs and challenged with 20 μM 2,5-di-tert-butylhydroquinone (TBHQ) in the absence of extracellular Ca2+ (n=3 independent experiments)"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Representative kinetics (G) and analysis (H) of aequorin-based [Ca2+]m measurements in intact ShRNA MICU1 HeLa stable clone cells transfected with the indicated constructs and challenged with 10 μM cyclopiazonic acid (CPA) in the presence of 100 μM EGTA (n=3 independent experiments)"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Resting mitochondrial calcium levels, evaluated through ratiometric imaging of the mitochondrial-targeted GCaMP6m, in MICU1-KO cells generated using the CRISPR/Cas9 technique and transfected with the indicated constructs (n=3 independent experiments; 30-56 cells)"
NCBI gene IDs:
"MICU1: 10367"