EMBO/SourceData huggingface dataset:
Figure legend: "HEK293 cells were transfected with siCTL or siDDX5. The next day, the cells that received siDDX5 were subsequently transfected with empty pcDNA3 vector (-), expression vectors encoding Flag-DDX5 (WT), or Flag-DDX5-RK (RK). The cells were subjected to Western blotting to visualize Flag-DDX WT and RK expression. Pol II ChIP and DRIP analysis of β-actin gene were performed. For the ChIP analysis, the Y-axis shows the signal-to-noise ratio of RNA Pol II IP relative to control IgG IP. The graphs show the average and S.E.M. from at least three independent experiments. Statistical significance was assessed using Student's t-test. *: p &lt; 0.05; **: p &lt; 0.01 and ****: p &lt; 0.0001."
NCBI gene IDs:
"Flag: 
β-actin: 60
DDX5: 1655"
Figure legend: "HEK293 cells were transfected with siCTL or siPRMT5. The cells were subjected to Western blotting, RNA Pol II ChIP and DRIP analysis of β-actin gene. The graphs show the average and S.E.M. from at least three independent experiments. Statistical significance was assessed using Student's t-test. *: p &lt; 0.05; **: p &lt; 0.01."
NCBI gene IDs:
"β-actin: 60
PRMT5: 10419"
Figure legend: "HEK293 cells were transfected with empty plasmid (-), wild type Flag-DDX5 (WT) or its RK mutant (RK) and subjected to western blot and Flag-DDX5 ChIP-qPCR analysis of β-actin gene. The graphs show the average and S.E.M. from at least three independent experiments. Statistical significance was assessed using Student's t-test. *: p &lt; 0.05; **: p &lt; 0.01 and ****: p &lt; 0.0001; n.s. not significant."
NCBI gene IDs:
"Flag: 
β-actin: 60
DDX5: 1655"
Figure legend: "B Immunoblot  showing CDK5RAP2  levels in Cdk5rap2  wild-type (WT), heterozygous (HET) and null erythroid progenitors (EPs) isolated from fetal livers. Actin  was used as loading control. ** indicates nonspecific band. C Quantification of mean protein levels from (B). Numbers in brackets correspond to number of embryos  analyzed. Data information: Bar graphs display mean ± s.d. Statistical analysis was based on the number of embryos  (C, Statistical significances were determined by One-way ANOVA test with Tukey's (C, test *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001."
NCBI gene IDs:
"Cdk5rap2: 214444"
Figure legend: "D Immunofluorescence  images of Cdk5rap2  WT, HET and null erythroid progenitors  isolated from fetal livers. Progenitors  were stained  for CDK5RAP2 (grey), γ-tubulin (magenta) and DNA   (Hoechst, blue). Images are maximum intensity projections of deconvolved z-stacks. Scale bar, 3 μm. Insets show higher magnification of centrosomes. Scale bar, 1 μm. E Quantification of mean centrosomal  signal intensities of CDK5RAP2  and γ-tubulin from (D). Numbers in brackets correspond to number of embryos  analyzed with a total number of 470 (WT), 406 (HET) and 379 (null) progenitors. Data information: Bar graphs display mean ± s.d. Statistical analysis was based on the number of embryos  E, Statistical significances were determined by One-way ANOVA test with Tukey's E, test *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001."
NCBI gene IDs:
"Cdk5rap2: 214444"
Figure legend: "C Immunofluorescence  images of  Cdk5rap2HET and Cdk5rap2null cells at 36 hours (T36) of ex vivo culture. Representative examples for different mitotic spindle morphologies are shown. Cells were stained  for α-tubulin (magenta),  γ-tubulin (grey), pHH3 (green) and DNA  (Hoechst, blue). Images are maximum intensity projections of deconvolved z-stacks. Scale bar, 2 μm. D Quantification of  mitotic spindle morphology at T24 or T36 of ex vivo culture. Graph depicts percentage of spindle  phenotypes. At T24, 3  Cdk5rap2WT (323 cells), 3 Cdk5rap2HET (296 cells) and 2  Cdk5rap2null (197 cells) embryos  were analyzed. At T36, 5 Cdk5rap2WT (387 cells), 6 Cdk5rap2HET (384 cells) and 3 Cdk5rap2null (202 cells) embryos were analyzed. Data information: Bar graphs display mean ± s.d. Statistical analysis was based on the number of embryos  (D, Statistical significance was determined by One-way ANOVA with Tukey's multiple comparisons test D, *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001."
NCBI gene IDs:
"Cdk5rap2: 214444"
Figure legend: "A Immunoblot  analysis of TP53, P27  and P21  levels in ex vivo cultured Cdk5rap2 wild-type (WT), heterozygous (HET) and null EBs  after 24 (T24) and 48 (T48) hours. GAPDH  was used as loading control. ** indicates unspecific band. B Quantification of mean protein levels from (A). Numbers in brackets represent number of embryos  analyzed. Data information: Box plots show 5th,95th (whiskers) and 25th, 50th and 75th percentile (boxes). All statistical analysis were based on number of embryos. All statistical significances were determined by One-way ANOVA with Tukey's multiple comparisons test.*P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001."
NCBI gene IDs:
"Cdk5rap2: 214444"
Figure legend: "B-E (B, D) Representative microscopy images of the mitochondrial network morphology in neurons of the amygdala (B) and the CA1 region of the hippocampus (D) in mitoCFP+/P301L- mice and mitoCFP+/P301L+ mice. MitoCFP signal is displayed in gray on the images. Gray scale bars: 5 µm. (C, E) Mitochondrial length in neurons of the amygdala (C) and in Ca1 (E) of mitoCFP+/P301L- and mitoCFP+/P301L+ mice. Data information: Values represent the mean ±SEM of n=11 MitoCFP+/P301L- mice and n= 13 MitoCFP+/P301L+ mice. Each gray open circle represents the mean of 6 technical replicates (data from 6 images) per animal. * P&lt;0.05, ** P&lt;0.01, Student unpaired t-test."
NCBI gene IDs:
"CFP: "
Figure legend: "F-I (F, H) Representative microscopy images of the endoplasmic reticulum stained with calnexin (in red) and mitochondria (mitoCFP) in neurons of the amygdala (F) and Ca1 (H) in mitoCFP+/P301L- and mitoCFP+/P301L+ mice. (G, I) Colocalization analysis between calnexin and mitoCFP in neurons of the amygdala (G) and Ca1 (I) of mitoCFP+/P301L- and mitoCFP+/P301L+ mice. The Manders' overlap coefficients M2 is represented on the graphs, as it indicates the proportion of overlaps between the mitoCFP signal (channel 2) and calnexin signal (channel 1) relative to the total pixel intensity of channel 2 (i.e. only in neurons, as mitoCFP is expressed only in these cells). Data information: Values represent the mean ±SEM of n=11 MitoCFP+/P301L- mice and n= 13 MitoCFP+/P301L+ mice. Each gray open circle represents the mean of 6 technical replicates (data from 6 images) per animal. * P&lt;0.05, ** P&lt;0.01, Student unpaired t-test."
NCBI gene IDs:
"CFP: "
Figure legend: "A Representative microscopy images (z-projections) of the mitochondrial network stained with TOMM20 in SH-SY5Y cells stably expressing a green fluorescent protein (GFP)-tagged wild-type form of tau protein (wtTau-GFP), a GFP-tagged P301L mutant form of tau protein (P301L-GFP) or cell expression the GFP only (control cells = WT-GFP). GFP signal is displayed in gray on the images, TOMM20 signal is in red, and the nuclei are delimited by white dotted lines. B-C Mitochondrial length (B) and percentage of mitochondria in the perinuclear area (C) in WT-GFP, wtTau-GFP and P301L-GFP expressing cells. On average 1,000-2,500 mitochondrial organelles were analyzed per group (n = 20-25 images per group, 3 independent experiments). Data information: Data are presented as mean ±SEM. White scale bars: 20 µm B-C * P&lt;0.05, ** P&lt;0.01, ***P&lt;0.001; One-way ANOVA + Tukey's post hoc test. TOMM20: translocase of the outer mitochondrial membrane complex subunit 20"
NCBI gene IDs:
"GFP: 
green fluorescent protein: 
Tau: 4137
tau: 4137"
Figure legend: "Visualization of the IP3R-VDAC (D), IP3R-GRP75 (E) interactions by in situ proximity ligation assay (PLA) WT-GFP, wtTau-GFP and P301L-GFP expressing cells. Interactions between the two targeted proteins are depicted in red (63X magnification) and cell surfaces are delimited by a white line. Data information: Data are presented as mean ±SEM. White scale bars: 20 µm GRP75: glucose-regulated protein 75 IP3R: inositol trisphosphate receptor VDAC: voltage-dependent anion channel."
NCBI gene IDs:
"GFP: 
Tau: 4137"
Figure legend: "G-I Quantitative analysis of the PLA signal represented as number of contact points between IP3R­VDAC (G), IP3R-GRP75 (H) and VAPB-PTPIP51 (I) per cell in percentage of the WT-GFP cells. In total, 60-75 cells were analyzed per group (5 independent experiments). Data information: Data are presented as mean ±SEM. White scale bars: 20 µm G-I * P&lt;0.05, ** P&lt;0.01, ***P&lt;0.001; One-way ANOVA + Tukey's post hoc test. GRP75: glucose-regulated protein 75 PTPIP51: protein tyrosine phosphatase-interacting protein 51 VDAC: voltage-dependent anion channel."
NCBI gene IDs:
"GFP: "
Figure legend: "K-L Mitochondrial length (K) and percentage of mitochondria in the perinuclear area (L) in WT cells and Tau KO cells. On average 1,000-2,500 mitochondrial organelles were analyzed per group (n = 20-25 images per group, 3 independent experiments). Data information: Data are presented as mean ±SEM. White scale bars: 20 µm * P&lt;0.05, ***P&lt;0.001; Student unpaired t-test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "P-R Visualization of the IP3R-VDAC (P), IP3R-GRP75 (Q) and VAPB-PTPIP51 (R) interactions by in situ PLA in WT and Tau KO cells. Interactions between the two targeted proteins are depicted in red (63X magnification) and cell surfaces are delimited by a white line. Data information: Data are presented as mean ±SEM. White scale bars: 20 µm * P&lt;0.05, ***P&lt;0.001; Student unpaired t-test. GRP75: glucose-regulated protein 75; IP3R: inositol trisphosphate receptor; PTPIP51: protein tyrosine phosphatase-interacting protein 51 VAPB: vesicle-associated membrane protein-associated protein B VDAC: voltage-dependent anion channel."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "G Quantification of total cholesterol levels in cell lysates from WT, P301L and Tau KO cells. Data information: (G Data are presented as mean ±SEM of n = 12-18 replicates per group (4 independent experiments) in percentage of the WT cells. * P&lt;0.05, ***P&lt;0.001; One-way ANOVA + Tukey's post hoc test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "K-N Mitochondrial cholesterol levels (K, L) and pregnenolone levels (M, N) in WT, P301L and Tau KO cells (K, M) and in brain preparations from WT and pR5 mice (L, N). Data information: K, M) Data are presented as mean ±SEM of n = 12-18 replicates per group (4 independent experiments) in percentage of the WT cells. * P&lt;0.05, ***P&lt;0.001; One-way ANOVA + Tukey's post hoc test. L, N) Data are presented as mean ±SEM of n = 6 WT and n = 5 pR5 mice with 2 technical replicates per animal. * P&lt;0.05, ** P&lt;0.01, Student unpaired t-test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "O-Q Quantification of total cholesterol levels (O), mitochondrial cholesterol levels (P) and pregnenolone levels (Q) in patient-derived induced pluripotent stem cells (iPSC) bearing a P301L tau mutation (iPSC-P301L) and the corresponding isogenic wild-type cells (iPSC-WT). (O-Q) Data are presented as mean ±SEM of n = 27 replicates/group (O), n = 9 replicates/group (P) and n = 18 replicates/group (Q). * P&lt;0.05, *** P&lt;0.001, Student unpaired t-test."
NCBI gene IDs:
"tau: 4137"
Figure legend: "A Visualization of the VAPB-PTPIP51 interactions by in situ proximity ligation assays (PLAs) in wild-type (WT) cells and P301L cells transfected with the control (Ctrl) siRNA, as well as in MFN2 siRNA transfected cells. Interactions between the two targeted proteins are depicted in red (63X magnification) and cell surfaces are delimited by a white line. White scale bars: 20 µm B Quantitative analysis of the PLA signal represented as number of contact points between VAPB-PTPIP51 per cell in percentage of the WT cells. In total, 60 cells were analyzed per group (4 independent experiments). Data information: Data are presented as mean ± SEM. * P&lt;0.05, ** P&lt;0.01, ***P&lt;0.001; One-way ANOVA + Tukey's post hoc test. PTPIP51: protein tyrosine phosphatase-interacting protein 51 VAPB: vesicle-associated membrane protein-associated protein B."
NCBI gene IDs:
"MFN2: 9927"
Figure legend: "A-D (A, C) Representative microscopy images (z-projections) of tau phosphorylation using (A) AT8 and (C) AT180 antibodies (in red) in GFP-tagged P301L cells (GFP signal in gray) treated for 24 h with CHIR99021 (CHIR) (right) and untreated cells (left). White scale bars: 25 µm. (B, D) Quantification of (B) AT8 and (D) AT180 staining fluorescence intensity. Data are presented as mean ±SEM of n = 50-60 cells per group (4 independent experiments). *** P&lt;0.001; Student unpaired t-test. Data information: Data are presented as mean ±SEM of n = 50 replicates per group (5 independent experiments) in percentage of the WT cells."
NCBI gene IDs:
"GFP: "
Figure legend: "E qRT-PCR of proliferation, stem cell and differentiation markers (CCND1, PTEN, SOX2, NOTCH1, NESTIN, MYC) in BT168FO cells after 48 h Dox treatment, compared to uninduced cells (n = 3; mean ± SD). Expression levels in non-induced cells were set as 1."
NCBI gene IDs:
"CCND1: 595
MYC: 4609
NESTIN: 10763
NOTCH1: 4851
PTEN: 5728
SOX2: 6657"
Figure legend: "A-B Seqminer heatmaps of MYC and Omomyc levels around TSSs of all MYC promoter-target genes in BT168FO (A) cells, treated or not with Dox for 24 h. TSS regions are ranked by decreasing MYC occupancy in untreated cells. Each row shows the ± 5 kb region centred on TSSs. Colour scaled intensities are in units of tags per 50bp. The plots adjacent to each heatmap depict MYC and Omomyc binding patterns at genes in the two gene clusters denoted by arrows, in cells treated or not with Dox."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "A-B Seqminer heatmaps of MYC and Omomyc levels around TSSs of all MYC promoter-target genes in U87FO (B) cells, treated or not with Dox for 24 h. TSS regions are ranked by decreasing MYC occupancy in untreated cells. Each row shows the ± 5 kb region centred on TSSs. Colour scaled intensities are in units of tags per 50bp. The plots adjacent to each heatmap depict MYC and Omomyc binding patterns at genes in the two gene clusters denoted by arrows, in cells treated or not with Dox."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "A Heatmap of RNA Pol II levels around TSSs of MYC promoter-target genes in BT168FO cells, treated or not with Dox for 24 h. TSS regions are ranked by decreasing MYC occupancy in untreated cells. Each row shows the ± 5 kb region centred on TSSs. Colour scaled intensities are in tags/50bp."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "B Tracks of Pol II binding signals (ChIP-seq) at NCL, miR-17-92, OLIG2, HDAC1, and DUSP10 genes in BT168FO cells treated or not with Dox for 24 h. The y-axis shows Pol II binding signals as tags/500bp/million reads. The x-axis displays genomic positions; introns as lines, exons as boxes. Arrowheads denote direction of transcription. Blue and green bars denote the presence of MYC or Omomyc peaks. Grey boxes are TSS regions."
NCBI gene IDs:
"DUSP10: 11221
HDAC1: 3065
miR-17-92: 407975
MYC: 4609
NCL: 4691
OLIG2: 10215"
Figure legend: "A Promoter occupancy by MYC correlates with transcript levels in cells untreated with doxycycline (black line). In doxycycline treated cells promoter occupancy by MYC is lower, and no longer increases together with transcript levels. The scatter plot displays MYCChIP-seq reads at promoters (-1000, +100 regions with respect to TSS) in BT168FO cells - untreated (black) or upon 48 h Dox treatment (blue) - versus transcript levels (FPKM from RNA-seq data)."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "D Transcript level distribution of MYC promoter-targets significantly down (red dots) or up (green dots) modulated in cells treated with Dox for 24 h vs. untreated cells. Transcript levels, expressed as FPKM, represent averages of three independent experiments."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "A RNA-seq expression values of select genes in BT168FO GSCs along a 48 h time course of Omomyc induction (n = 3 independent biological replicates). The block on the left represents DUSP (dual specificity phosphatase) family genes. The middle block contains transcription factors (TFs): the upper thirteen (ASCL1-SALL1) are GSC-specific and the remaining ones are oncogenic. The right block contains genes, including well-known MYC targets, with functions in proliferation, neurodifferentiation, and gliomagenesis. The first column of each block represents the average expression (log2 FPKM) in untreated cells (0 h) in the colour scale illustrated by the lower bar: violet indicates low and blue high expression. The other columns depict relative expression versus untreated cells (average log2 FC) at different times (8-48h) of Dox treatment, according to the scale shown by the upper bar: red indicating low and green high expression. The table in the upper left summarizes the GSEA score of the set 19 GSC-specific transcription factors [40], showing a significant downregulation by Omomyc."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "B Enrichment plots obtained by GSEA (Gene Set Enrichment Analysis) of RNA-seq data from BT168FO cells. Left: genes downregulated by Omomyc in BT168FO identify the set of genes targeted by the GSC core TFs [40]. Middle: Omomyc downregulated genes identify a gene set upregulated by MYC in cancer cells [66]. Right: genes upregulated by Omomyc identify genes downregulated in small cell lung cancers carrying MYC amplification [2]. ."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "C RT-qPCR expression analysis of selected miRs in U87FO cells grown 48 h in the presence (+) or absence (-) of Dox (n = 3; mean ± SD). Expression levels were normalized using U6RNA as control. Expression levels in non-induced cells were set as 1."
NCBI gene IDs:
"U6: "
Figure legend: "E EGFR, ZEB1, and GAPDHimmunoblots in uninduced U87FO cells (-) and in cells transfected with LNA oligos targeting miR-200a-200b-429 (LNA 200) and miR-146b (LNA 146b), or with a non-targeting LNA (C), and induced with Dox for 48 h (+Dox). Representative images are shown."
NCBI gene IDs:
"miR-146b: 574447"
Figure legend: "F MTS cell proliferation assay of uninduced or Dox induced U87FO cells, transfected with either a non-targeting LNA (LNA C), or LNA oligos targeting the miR-200a-200b-429 cluster (LNA 200), miR-146b (LNA 146b), or both (LNAmix) and analysed at 24, 48 and 72h (n = 3; mean ± SD)."
NCBI gene IDs:
"miR-146b: 574447"
Figure legend: "(A). Cell growth assessment of ΔIndLon (upper plot) and ΔIndFtsH (lower plot) mutants grown under inducing (blue) or depleting conditions (red). Growth was monitored by measuring DNA and protein biomass over time. The average from two independent biological replicates is shown."
NCBI gene IDs:
"FtsH: 45596573
Lon: 45596210"
Figure legend: "(D). Role of Lon and FtsH under heat shock stress conditions. ΔIndLon and ΔIndFtsH mutants were grown under inducing (Lon+ or FtsH+) or depleting conditions (Lon+ or FtsH+, 60h of depletion for both, Lon and FtsH), and then exposed at 45°C during 0, 5, 10, 15 or 20 min. Then, growth after the heat treatment was monitored over time under inducing conditions by the 430/560 absorbance rate index that shows pH changes in the medium. The average from two independent biological replicates is shown for each condition."
NCBI gene IDs:
"FtsH: 45596573
Lon: 45596210"
Figure legend: "(C) In vivo degradation assays of FtsA, FtsZ and DnaB. ΔIndLon mutants expressing the different N-terminal FLAG-tagged derivatives were grown in depleting conditions for 36h. Then, Lon expression was transiently induced for 3h before translation was blocked with gentamicin (Gm). Samples were taken at the indicated time points after gentamicin treatment, and processed for immunoblot analysis using anti-FLAG antibodies. LC, loading control. As controls, non-induced cells were also treated with gentamicin and processed at the same time points. Immunoblots are representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
Lon: 45596210"
Figure legend: "(I) Similar to panel C, but for FtsH candidate substrates. In this case, C-terminal FLAG-tagged derivatives were grown in depleting conditions for 60h before transient expression of FtsH for 3h and gentamicin treatment."
NCBI gene IDs:
"FLAG: 
FtsH: 45596573"
Figure legend: "(B) Protein stability assessment of Lon substrates containing multiple or single mutations in putative Lon degrons. N-terminal FLAG-tagged derivatives with mutations shown in panel A were expressed in the ΔIndLon mutant. Protein levels were then determined by immunoblot using anti-Lon and anti-FLAG antibodies comparing inducing (+) or depleting (-) conditions (48h of depletion). LC, loading control."
NCBI gene IDs:
"Lon: 45596210"
Figure legend: "(B) Lon surveillance of truncated protein variants. As an example, an N-terminal FLAG-tagged derivative of MPN304, which encodes a truncated variant of ArcA (ArcA-Nt), was expressed in the ΔIndLon mutant, and its accumulation monitored over time under non-inducing conditions using anti-FLAG antibodies. Anti-Lon antibodies were used to monitor Lon depletion along the time course experiment (western blot figure on left). MPN304 stability was also assessed by in vivo degradation experiments in Lon depleted mutants (36h of depletion), in which protein expression was blocked with gentamicin (Gm) during the indicated time points after transient induction of Lon for 3h. As control, samples after Gm treatment were also taken from non-induced samples. LC, loading control (western blot figure on right). Immunoblots are representative of two independent experiments. A schematic representation of the arginine deiminase pathway, which is inactive in M. pneumoniae, and the estimated protein copy number of its components are also shown for the ΔIndLon mutant grown in inducing (+) or depleting (-) conditions."
NCBI gene IDs:
"MPN304: 
Lon: 45596210"
Figure legend: "(E) Protein quality control of terminal organelle assembly mediated by Lon. Top of panel E, a schematic representation of the genetic organization at the P65 operon after deleting the N-terminal region of the hmw2 gene in the ΔIndLon mutant. The expected PCR products for the deletion are also indicated. Below, an agarose electrophoresis gel showing the PCR validation of the intended deletion, and SDS-PAGE and immunoblot analyses monitoring the expression of specific terminal organelle proteins, whose stability depend on HMW2, for ΔIndLon or ΔIndLon_Δhmw2Nt cell lysates grown in inducing (+) or depleting (-) conditions (48h of depletion). LC, loading control."
NCBI gene IDs:
"hmw2: 45596187
HMW2: 45596187
Lon: 45596210"
Figure legend: "16 days after tumor inoculation, B16F10 tumors from WT and Ythdf2 cKO mice were digested for flow cytometry analysis. D, Percentage (left) and absolute numbers (right) of CD8+ T cells in tumors (n=4). Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "16 days after tumor inoculation, B16F10 tumors from WT and Ythdf2 cKO mice were digested for flow cytometry analysis. E, Representative plots (left) and the percentage (right) of IFNγ producing CD8+ T cells (n=5). Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "16 days after tumor inoculation, B16F10 tumors from WT and Ythdf2 cKO mice were digested for flow cytometry analysis. G, Percentage (left) and absolute numbers (right) of Treg cells (n=4). Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "16 days after tumor inoculation, B16F10 tumors from WT and Ythdf2 cKO mice were digested for flow cytometry analysis. I, The proportion of Treg cells expressing the indicated surface marker (n=5). Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"