EMBO/SourceData huggingface dataset:
Figure legend: "(G) and (H). Depletion of PARP1 in HT-29 and Capan-1/TP cells resulted in a decrease in DNA damage and thioparib resistance. The upper panel shows the changes in γH2AX and phospho-RPA32 in HT-29 PARP1 knockout (KO) clones (G) or Capan-1/TP PARP1 KO clones (H) and their parental cells after exposure to thioparib for 24 h. The lower panel shows the IC50 values of thioparib in these PARP1 KO clones and parental cells. Cells were treated with thioparib for 5 or 7 d and the IC50 values were determined by SRB assay from three separate experiments. Data are presented as the mean ± SD. ThP: Thioparib, OP: Olaparib, TP: Talazoparib, 391: Cpd 391."
NCBI gene IDs:
"PARP1: 142"
Figure legend: "(A) HR repair assays in U2OS-DR-GFP cells treated with increasing concentrations of thioparib. Cells were incubated with the indicated drugs at the time of I-SceI introduction for 48 h. GFP-positive cells were analyzed after I-SceI transfection for 72 h by flow cytometry. The ATR inhibitor VE-821 was used as a positive control. Three independent experiments were performed and data are expressed as mean ± SD. Statistical analysis was performed by one-way ANOVA, and all groups were compared with control. ***P &lt; 0.0001, *P = 0.0205, 0.0101 (from left to right)."
NCBI gene IDs:
"I-SceI: 854590"
Figure legend: "(H) HR repair assay. U2OS-DR-GFP (WT) and PARP1 KO cells (KO1 and KO2) were treated with the indicated drugs for 48 h and collected after 72 h of I-SceI transfection as in (A). VE-821 served as a positive control. PARP1 knockout efficiency was evaluated by western blotting. Data from three biological replicates are presented as mean ± SD. Statistical analysis was performed by one-way ANOVA, and each group was compared with the control. ***P &lt; 0.0001, *P = 0.0143, 0.0483, ns: P = 0.3061, 0.8269, 0.9997, 0.3906 (from left to right)."
NCBI gene IDs:
"PARP1: 142
I-SceI: 854590"
Figure legend: "(C) Effects of thioparib on CXCL9, CXCL10, and IL15 mRNA in JeKo-1 cells. Data from three independent experiments are shown as the mean ± SD. Statistical analysis was performed by two-way ANOVA. Left panel: *P = 0.0367, **P = 0.0011; median panel: ***P = 0.0002, &lt; 0.0001, &lt; 0.0001; right panel: ***P = 0.0005, 0.0009, 0.0001 (from left to right)."
NCBI gene IDs:
"CXCL10: 3627
CXCL9: 4283
IL15: 3600"
Figure legend: "(D) The protein levels of p-STAT1, p-TBK1, γH2AX, and phospho-RPA32 in HT-29 parent, PARP1−/− (#KO1), and PARP7−/− cell lines after 24 or 48 h thioparib treatment."
NCBI gene IDs:
"PARP1: 142
PARP7: 25976"
Figure legend: "(F) Western blot analysis of p-STAT1, p-TBK1, and γH2AX in HT-29 parent, STING−/−, and TBK1−/−cells after 24 or 48 h thioparib treatment."
NCBI gene IDs:
"STING: 340061
TBK1: 29110"
Figure legend: "THP-1 derived macrophages were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. Viral protein expression for PB1-F2 (A) was detected by immunoblotting at 24 h post-infection (hpi) Equal loading was confirmed by detection of beta actin."
NCBI gene IDs:
"F2: "
Figure legend: "B THP-1 derived macrophages were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. Viral protein expression for NP (B) was detected by immunoblotting at indicated time points, respectively. Equal loading was confirmed by detection of beta actin."
NCBI gene IDs:
"F2: "
Figure legend: "(C) THP-1 derived macrophages were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. Levels of viral M1 mRNA expression were detected by RT q‑PCR at 16 hpi. The mean ± standard deviation of three independent biological samples is shown. Statistical analysis was performed by students t-test."
NCBI gene IDs:
"F2: 
M1: "
Figure legend: "(D) THP-1 derived macrophages were infected with VN and PR8 Wt and ΔF2 strains for 24 h at MOI 10. Levels of IL-1β were quantified by ELISA from the supernatants. The mean ± standard deviation of seven independent experiments is shown. Statistical analysis was performed by one-way ANOVA, p-values are indicated."
NCBI gene IDs:
"F2: "
Figure legend: "(E) THP-1 control and knockout cells for NLRP3 were mock treated or infected with the VN Wt and ΔF2 strains for 24 h at MOI 10 and IL-1β levels were assessed by ELISA from the supernatants. The mean ± standard deviation of five independent experiments is shown. Statistical analysis was performed by one-way ANOVA, p-values are indicated."
NCBI gene IDs:
"F2: 
NLRP3: 114548"
Figure legend: "(F) Immunoblot detection for NLRP3 on control and NLRP3 knockout THP-1 cells. Equal loading was confirmed by specific blotting against beta actin. Representative blot of three independent repeats is shown."
NCBI gene IDs:
"beta actin: 
NLRP3: 114548"
Figure legend: "(G) Human primary macrophages differentiated from PBMCs from two healthy donors were infected with H5N1 Wt and ΔF2 for 5 h at MOI 10. Levels of IL-1β were measured by ELISA in technical replicates. The mean of two independent experiments is shown. Statistical analysis was performed by one-way ANOVA, p-values are indicated."
NCBI gene IDs:
"F2: "
Figure legend: "(H) THP-1 derived macrophages were infected with a human H3N2 IAV strain (Wyo) Wt and ΔF2 for 24 h at MOI 4. Levels of IL-1β were measured by ELISA from the supernatants. The mean ± standard deviation of four independent experiments is shown. Statistical analysis was performed by one-way ANOVA, p-values are indicated."
NCBI gene IDs:
"F2: "
Figure legend: "(I) Detection of viral matrix protein M1 by immunoblotting in cells infected with the H3N2 Wt and ΔF2 strains."
NCBI gene IDs:
"F2: "
Figure legend: "(J) Human primary macrophages isolated and differentiated from PBMCs from three healthy donors were infected with H3N2 Wt and ΔF2 for 24 h at MOI 4. Levels of IL-1β were measured by ELISA. The mean ± standard deviation of three independent experiments is shown. Statistical analysis was performed by one-way ANOVA, p-values are indicated."
NCBI gene IDs:
"F2: "
Figure legend: "Primary human alveolar macrophages from BALF of five pediatric patients (patients are color coded) were infected with the human H3N2 strain (Wyo) Wt and ΔF2 for 16 h at MOI 1. (K) Levels of IL-1β were measured by ELISA from the supernatants. The mean ± standard deviation is shown."
NCBI gene IDs:
"F2: "
Figure legend: "Primary human alveolar macrophages from BALF of five pediatric patients (patients are color coded) were infected with the human H3N2 strain (Wyo) Wt and ΔF2 for 16 h at MOI 1. Levels of viral M1 mRNA (L), Mx1 mRNA (M) and IP10 mRNA (N) expression were detected by RT q‑PCR. The mean ± standard deviation for cells from four patients are shown. Individiual patients are color coded."
NCBI gene IDs:
"F2: 
M1: 
IP10: 3627
Mx1: 4599"
Figure legend: "(A-D) THP-1 cells were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. Flow cytometry results for annexin V single positive (A and C) and annexin V and zombie dye double positive (B and D) cells. Percentage of positive cells are depicted at 12 hpi or 24hpi."
NCBI gene IDs:
"F2: "
Figure legend: "(E and F) THP-1 cells were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. LDH release measured from supernatants of mock treated or infected cells at 12 hpi (E) or 24hpi (F)."
NCBI gene IDs:
"F2: "
Figure legend: "G THP-1 Caspase 1 -/- cells were infected with the wildtype (Wt) and ΔF2 H5N1 (VN) virus at MOI 10. LDH release measured from supernatants of mock treated or infected cells at 12 hpi or 24hpi. n=3 for 12hpi, n=5 for 24hpi. Medians are indicated, statistical analysis was performed by one-way ANOVA."
NCBI gene IDs:
"F2: 
Caspase 1: 834"
Figure legend: "(A) THP-1 cells infected with VN Wt and ΔF2 virus at a MOI 10 for 24 h or treated with 6.7 μM of nigericin (nig) for 45 min were analyzed by immunoblotting. Detection of the full-length gasdermin D. Equal loading was confirmed by beta actin. One representative blot of five independent repeats is shown. (B) Quantification of band intensity for the gasdermin D full-length isoform. The mean ± standard deviation five independent experiments is shown. Statistical analysis was performed by the paired two‑tailed Student's t test., The asterisk (*) marks a significant difference when compared to the mock group ** p &lt; 0.01. "
NCBI gene IDs:
"F2: "
Figure legend: "(C) THP-1 cells infected with VN Wt and ΔF2 virus at a MOI 10 for 24 h or treated with 6.7 μM of nigericin (nig) for 45 min were analyzed by immunoblotting. Detection of cleaved gasdermin D N-terminal and C-terminal isoforms by immunoblot in supernatants; one representative experiment of three independent repeats is shown. (D) Quantification of band intensity for N-terminal gasdermin D isoform band. Three independent experiments are shown. Statistical analysis was performed by the paired two‑tailed Student's t test., * p &lt; 0.05. (E) Quantification of band intensity for C-terminal gasdermin D isoform band. Three independent experiments are shown. Statistical analysis was performed by the paired two‑tailed Student's t test., * p &lt; 0.05. "
NCBI gene IDs:
"F2: "
Figure legend: "(F) THP-1 cells infected with VN Wt and ΔF2 virus at a MOI 10 for 24 h or treated with 6.7 μM of nigericin (nig) for 45 min were analyzed by immunoblotting. Pro-caspase-1 and active caspase-1 were detected by immunoblot in cell lysates (cell, upper panels) and supernatants (sup, lower panels) from the same experiment. One representative experiment of three independent repeats is shown. (G) Quantification of band intensity for active caspase-1 band. Five independent experiments are shown. Statistical analysis was performed by the paired two‑tailed Student's t test. * p &lt; 0.05. (H) Levels of total pro-caspase-1 were quantified on pellets and respective supernatants. Three independent experiments are shown. Statistical analysis was performed by the paired two‑tailed Student's t test. "
NCBI gene IDs:
"F2: "
Figure legend: "(A) Western blot analysis of whole cell lysate (input) and Flag-specific immunoprecipitation (IP) from 293T cells co-transfected with expression plasmids encoding V5-NLRP3 and Flag ZsGreen-PB1-F2 from indicated viral strains for 24 h Representative blot of three independent repeats is shown. (B) Quantification of the NLRP3 IP band was normalized to the respective NLRP3 input band (n=3 independent biological replicates, normalized to the respective input). Mean values +- SD are indicated. "
NCBI gene IDs:
"Flag: 
PB1-F2: 
V5: 
ZsGreen: 
NLRP3: 114548"
Figure legend: "(E) 293T cells co transfected with expression plasmids encoding different truncation mutants for Flag Zsgreen-PB1-F2 from VN (H5N1) and V5-NLRP3 were processed as described in (A). Representative blot of three independent repeats is shown."
NCBI gene IDs:
"Flag: 
PB1-F2: 
V5: 
Zsgreen: 
NLRP3: 114548"
Figure legend: "(B) 293T cells were co-transfected with expression plasmids encoding for the indicated V5-tagged domains of NLRP3 for 24h. Whole cell lysate (left blot) and flag specific IP (right blot) were analyzed by immunoblotting. Equal loading was confirmed by blotting against tubulin. Representative blots of two independent experiments are shown."
NCBI gene IDs:
"flag: 
V5: 
NLRP3: 114548"
Figure legend: "(C) 293T cells were co‑transfected with expression plasmids encoding Flag-NLRP3, V5‑PB1-F2 and V5‑ASC for 24h. Whole cell lysate and flag specific IP were analyzed by immunoblotting for indicated antigens. Equal loading of whole cell lysate was assured by blotting against beta actin. Representative blot of two independent repeats is shown."
NCBI gene IDs:
"Flag: 
PB1-F2: 
V5: 
NLRP3: 114548
ASC: 29108"
Figure legend: "(D) 293T cells were co-transfected with expression plasmids encoding for Flag-NLRP3, PB1-F2 or NEK7 or combinations thereof as indicated. Whole cell lysate and Flag IP were analyzed by immunoblotting. Equal loading was confirmed by blotting against beta actin. Representative blots of three independent experiments are shown. Right panel: Quantification of IP bands' intensity relative to their input. The mean ± standard deviation of three independent experiments is shown. Statistical analysis was performed by paired two‑tailed Student's t test (*p&lt; 0.05)."
NCBI gene IDs:
"Flag: 
PB1-F2: 
NEK7: 140609
NLRP3: 114548"
Figure legend: "(E) As in D: 293T cells were co-transfected with expression plasmids encoding for Flag-NLRP3-LRR, PB1-F2 or NEK7 or combinations thereof as indicated. Whole cell lysate and NLRP3-LRR IP were analyzed by immunoblotting. Equal loading was confirmed by blotting against beta actin. Representative blots of three independent experiments are shown. (F) Quantification of IP bands' intensity relative to their input. The mean ± standard deviation of three independent experiments is shown. Statistical analysis was performed by paired two‑tailed Student's t test (*p&lt; 0.05). "
NCBI gene IDs:
"Flag: 
PB1-F2: 
NEK7: 140609
NLRP3: 114548"
Figure legend: "G) As in D and E: 293T cells were co-transfected with expression plasmids encoding for Flag-NLRP3-LRR, PB1-F2 or V5-NLRP3-Pyrin or combinations thereof as indicated. Whole cell lysate and NLRP3-LRR IP were analyzed by immunoblotting. Equal loading was confirmed by blotting against tubulin. Representative blots of three independent experiments are shown."
NCBI gene IDs:
"Flag: 
PB1-F2: 
V5: 
NLRP3: 114548"
Figure legend: "Colony formation assay of miRNA mimic transfected-Dgcr8-/- ESCs. There were 17 miRNAs that decreased the colony forming ability, 15 miRNAs that did not affect colony formation and 8 miRNAs enhanced colony formation of ESCs. Representative pictures are shown. Full data are shown in Supplementary Fig S1."
NCBI gene IDs:
"Dgcr8: 94223"
Figure legend: "Cell cycle distribution in representative miRNAs evoking G1 phase accumulation in miRNA mimic transfected Dgcr8-/- ESCs. Error bars indicate s. d. (n=3). Full data are shown in Supplementary Fig S3A, B."
NCBI gene IDs:
"Dgcr8: 94223"
Figure legend: "B,C. Northern blot analysis of the expression of miR-23a, miR-27a and miR-24 during EB formation (B) and RA induced differentiation of V6.5 ESCs (C). The heatmap was representive of the results of quantitative analysis."
NCBI gene IDs:
"miR-23a: RF00642
miR-24: RF00178
miR-27a: 407018///RF00644"
Figure legend: "D,E. The expression of primary miR-23a~27a~24-2 transcript during EB formation (D) and RA induced differentiation (E). Error bars indicate s.d. (n=3)."
NCBI gene IDs:
"miR-23a: RF00642"
Figure legend: "D,E. The expression of primary miR-23a~27a~24-2 transcript during EB formation (D) and RA induced differentiation (E). Error bars indicate s.d. (n=3)."
NCBI gene IDs:
"miR-23a: 387216"
Figure legend: "F. Q-PCR analysis of miR-23a, miR-27a and miR-24 expression in ESCs (JM8A3) and a variety of C57BL/6J adult tissues. Data are shown as mean + s.d. (n=3)."
NCBI gene IDs:
"miR-23a: RF00642
miR-24: RF00178
miR-27a: RF00644"
Figure legend: "G. Relative protein levels of Oct4, Sox2 and Nanog in miRNA mimic transfected Dgcr8-/- ESCs and wild- type ESCs."
NCBI gene IDs:
"Dgcr8: 94223"
Figure legend: "H,I. Oct4, Nanog, Foxa2, Gata2 and Nestin immunofluorescence staining of mimic transfected Dgcr8-/- (H) and wild- type ESCs(I)."
NCBI gene IDs:
"Dgcr8: 94223"
Figure legend: "Western blot analysis of the expression of target genes in miR-27a or miR-24 over-expressed V6.5 ESCs. Candidate target genes whose expression was decreased were framed."
NCBI gene IDs:
"miR-24: RF00178
miR-27a: RF00644"
Figure legend: "The relative luciferase activity of the reporter constructs co-transfected with scramble or miR-27a and miR-24 mimics. Error bars indicate s.d. (n=3); * indicates P-value &lt; 0.05. Candidate target genes whose luciferase activity was decreased were framed. PC group was positive reporter control which harbored reverse complementary sequence of miR-27a or miR-24."
NCBI gene IDs:
"miR-24: RF00178
miR-27a: 407018"
Figure legend: "The relative luciferase activity of the wild-type 3′ UTR or mutated 3′ UTR reporter co-transfected with scramble or miR-27a and miR-24 mimics. Data are shown as mean + s.d. (n=3); * indicates P-value &lt; 0.05."
NCBI gene IDs:
"miR-24: RF00178
miR-27a: 407018"
Figure legend: "I. Q-PCR analysis of primary-23a~27a~24-2, miR-23a, miR-27a and miR-24 expression in c-Myc knocked down ESCs. Error bars indicate s.d. (n=3); * indicates P-value &lt; 0.05; ** indicates P-value &lt; 0.01; *** indicates P-value &lt; 0.001."
NCBI gene IDs:
"miR-23a: RF00642
miR-24: RF00178
miR-27a: RF00644
c-Myc: 17869"
Figure legend: "L. Relative luciferase activity of the wild-type and -15 binding site mutated promoter in NIH3T3 cells co-transfected with c-Myc or the blank vector (NC). Error bars indicate s.d. (n=3); * indicates P-value &lt; 0.05."
NCBI gene IDs:
"c-Myc: 17869"
Figure legend: "AP staining of the iPSC colonies in reprogramming with OSK combined with scramble control, inhibitors of miR-23a, miR-27a, miR-24 and let-7c respectively."
NCBI gene IDs:
"let-7c: RF00027
miR-23a: RF00642
miR-24: RF00178
miR-27a: RF00644"
Figure legend: "Detection of endogenous and exogenous Oct4, Sox2, Klf4 and Nanog expression in iPSCs, ESCs and MEFs by RT-PCR."
NCBI gene IDs:
"Klf4: 16600
Nanog: 71950
Oct4: 18999
Sox2: 20674"
Figure legend: "Q-PCR analysis of the expression of self-renewal (Oct4, Sox2, Nanog) and differentiation (Cdx2, Foxa2, Brachyury, Hand1, Nestin, FGF5) relative markers in EBs derived from the iPSCs. Error bars indicate s.d. (n=3)."
NCBI gene IDs:
"Cdx2: 12591
FGF5: 14176
Foxa2: 15376
Hand1: 15110
Nanog: 71950
Nestin: 18008
Oct4: 18999
Sox2: 20674
Brachyury: 20997"
Figure legend: "Genomic PCR of the specific fragments spanning miR-23~27~24 clusters. WT indicate wild-type ESCs; DKO indicate miR-23a~27a~24-2-/-, miR-23b~27b~24-1-/- ESCs; KO indicate miR-23a~27a~24-2-/- ESCs."
NCBI gene IDs:
"miR-23a: RF00642"
Figure legend: "Real-time PCR analysis of mature miR-23a/b, miR-27a/b and miR-24 expression in wild-type, DKO and KO ESCs. Data are shown as mean + s.d. (n=3)."
NCBI gene IDs:
"miR-23a: RF00642
miR-24: RF00178
miR-27a: RF00644"
Figure legend: "Real-time PCR analysis of Oct4, Sox2 and Nanog expression in wild-type and miR-23~27~24 cluster knockout ESCs. Data are shown as mean + s.d. (n=3)."
NCBI gene IDs:
"Nanog: 71950
Oct4: 18999
Sox2: 20674"
Figure legend: "Real-time PCR analysis of Oct4 and several lineage differentiation markers expression during EB formation of wild-type and knockout ESCs. Data are shown as mean ± s.d. (n=3)."
NCBI gene IDs:
"Oct4: 18999"
Figure legend: "A The mRNA expression of FAM189A2/ENTREP in the primary human mammary epithelium HMEC and human breast cancer  cell lines. In  qRT-PCR analyses, the  expression was normalized to the immortalized normal human mammary epithelium HMEC4tertshp16. Relative expression ratios shown as mean + SD from three biological replicates."
NCBI gene IDs:
"ENTREP: 9413
FAM189A2: 9413"
Figure legend: "B Relative expression of FAM189A2/ENTREP in the normal (white) and cancer (green) tissues of breast (Curtis  Breast), lung (Okayama Lung), colorectal (TCGA Colorectal) and  head  and neck (Estilo Head-Neck). Data were downloaded from the Oncomine database P-values obtained by Student's t-tests and the number of cases are listed on the top and bottom of figures, respectively. P &lt; 0.05 was considered as statistically significant. Box-whisker plot represents the interquartile range (25th and 75th percentiles) as a box and the median as a line. The maximn and minimun values within 1.5 x interquartile range are shown as whiskers. Outlier data are plotted as dots. C Relative  expression of FAM189A2/ENTREP in the primary (green) and the metastatic sites (blue) including lymph node, bone, liver,  lung, soft tissues of prostatic cancer (Grasso  Prostate) (left) and in the normal (white), primary (green) and metastatic sites (three cases of sentinel lymph node) (blue) of breast cancer (TCGA Breast) (right). Note that only three cases of the  metastatic breast cancer were available in the dataset. Data were downloaded from the Oncomine database P-values obtained by Student's t-tests and the number of cases are listed on the top and bottom of figures, respectively. P &lt; 0.05 was considered as statistically significant. Box-whisker plot represents the interquartile range (25th and 75th percentiles) as a box and the median as a line. The maximn and minimun values within 1.5 x interquartile range are shown as whiskers. Outlier data are plotted as dots."
NCBI gene IDs:
"ENTREP: 9413
FAM189A2: 9413"
Figure legend: "B, C The immunoprecipitation analysis. Co-precipitation was impaired by either the WW domain deletion of ITCH or the mutation in the PPxY motifs of ENTREP. Data shown are representative of at least two independent experiments. IP, immunoprecipitation; IB, immunoblot."
NCBI gene IDs:
"ENTREP: 9413
ITCH: 83737"
Figure legend: "A The  nickel-pull down assay using  myc/HIS-tagged ENTREP along with HA-tagged ubiquitin. Arrows indicate  HA-ubiquitin-incorporating ENTREP. Arrowhead indicates ENTREP without ubiquitination. Data shown are representative of three independent experiments."
NCBI gene IDs:
"HA: 
HIS: 
myc: 
ENTREP: 9413"
Figure legend: "A The  immunoprecipitation  analysis. The cytoplasmic ENTREP (wild or mut1+2 mutant)  co-precipitated with EPN1myc in the presence of ITCH wild or C830A. The  blot  bands were semi-quantified using ImageJ software. ENTREP ratio was caluculated as a ratio of  ENTREP  in the myc-coprecipitaed sample to that of the total lysate. The ratios shown as mean ± SD from three biological replicates. P-values were obtained by Student's t-tests and P &lt; 0.005 was considered as statistically significant. No significant difference was observed."
NCBI gene IDs:
"ITCH: 83737"
Figure legend: "F The nickel-pull down assay using indicated vectors along with HISx6-tagged wild type ubiquitin vector. HA-tagged DsRed was used as a control for HA-CXCR4DD-DsRed. Arrowhead,  FLAG-tagged ITCH (wild or C830A); arrow, FLAG-ENTREP  wild cyt. Data shown are representative of at least two independent experiments. G Co-localization of ENTREP  and CXCR4 in the  endosome.  Cos7  cells were transfected with ENTREP-FLAG, CXCR4-DsRed and Halo-ITCH wild vectors and treated with  CXCL12. ENTREP-FLAG and CXCR4-DsRed co-localized in the RAB7-positive  endosome. Inserts, the high-magnification images of the marked area indicating the  colocalizaion of ENTREP-FLAG, CXCR4-DsRed and RAB7. Scale bar 10mm. The image is representative of at least two independent experiments."
NCBI gene IDs:
"DsRed: 
FLAG: 
HA: 
Halo: 
HIS: 
CXCR4: 7852
ENTREP: 9413
ITCH: 83737"
Figure legend: "C The expression of endogenous CXCR4  in  lentivirally-transduced MCF-7 ENTREP-KO cells. CXCL12 (+) and (-) indicate the presence and absence of CXCL12  treatment. In CXCL12-treated ENTREP-EGFP expressing cells, a non-negligible amount of CXCR4 was observed in the  cytoplasm, in which CXCR4 was overlapped with ENTREP-EGFP. CXCR4 expression on the cell surface still remained (arrows). Scale bar 10 mm. The images are representative of at least three independet experiments."
NCBI gene IDs:
"EGFP: 
ENTREP: 9413"
Figure legend: "A Dot plot presentation of the chemotaxis  analyses using the Boyden chamber. CXCL12 (+) and (-) indicate the presence and absence of CXCL12  as an attractant in the bottom chambers. ENTREP-knockout enhanced the chemotaxis toward CXCL12, which was blocked by pre-treatment with AMD3100 (upper panel). The doxycyclin (DOX)-induced  ENTREP expression suppressed the chemotaxis  of mouse 4T1-Luc  cells toward  CXCL12 (lower panel). Ratio of  migrated cells into the bottom chamber against the applied cells on the top chambers was normalized by the results of non-treated cells (n=7, upper panel, and n=9, lower panel, from at least two independent experiments, respectively). P-values were obtained by Student's t-tests and P &lt; 0.005 was considered as statistically significant. Insert, the  immunoblot  analysis of the DOX-induced ENTREP expression in 4T1-Luc cells. NS, not significant."
NCBI gene IDs:
"ENTREP: 9413
ENTREP: 381217"
Figure legend: "(B) Body weight comparison over time of Ndufs3 smKO male mice (filled blue squares; n=14) and age matched wild-type (WTFlx; n=15) males (empty blue squares). Ndufs3 smKO females (filled red circles) showed similar results (n=10 for smKO, and n=12 for WTFlx group). P values were calculated by Student's t test;"
NCBI gene IDs:
"Ndufs3: 68349"