EMBO/SourceData huggingface dataset:
Figure legend: "B. Analysis of relative TNFSF2 protein release in human control and SIGLEC11/16 knockout THP1-macrophages. The released protein levels were reduced after 24 hours of co-treatment with LPS (1 µg/ml) and concentrations of 0.15 µM and 1.5 µM of polySia avDP20 in the human wild type macrophages. No response to polySia avDP20 was detectable in the knockout line. Data show mean +/- SEM. ** p &lt; 0.01, *** p &lt; 0.001, ANOVA followed by Bonferroni. Statistical analysis was done in relation to the LPS control. WT: no treatment n=8 and p&lt;0.0001, PolySia avDP20 1.5 µM n=5 and p&lt;0.001, LPS n=7, LPS/PolySia avDP20 0.15µM n=5 and p=0.002, LPS/PolySia avDP20 1.5µM n=4 and p=0.0003. Siglec11/16 KO: no treatment n=6 and p&lt;0.001, PolySia avDP20 1.5 µM n=5 and p&lt;0.001, LPS n=7, LPS/PolySia avDP20 0.15µM n=5 and p=1.0, LPS/PolySia avDP20 1.5µM n=5 and p=1.0."
NCBI gene IDs:
"SIGLEC11: 114132"
Figure legend: "C. Analysis of relative VEGFA gene transcription in human control and SIGLEC11/16 knockout THP1-macrophages. Gene transcripts were reduced after 24 hours of co-treatment with LPS (1 µg/ml) and polySia avDP20 (0.15 µM and 1.5 µM) in the human wild type macrophages. No response to polySia avDP20 was detectable in the knockout macrophages. Data show mean +/- SEM. *** p &lt; 0.001, ANOVA followed by Bonferroni. Statistical analysis was done in relation to the LPS control. WT: no treatment n=6 and p&lt;0.0001, PolySia avDP20 1.5 µM n=5 and p&lt;0.0001, LPS n=5, LPS/PolySia avDP20 0.15µM n=5 and p=0.0002, LPS/PolySia avDP20 1.5µM n=5 and p&lt;0.0001. Siglec11/16 KO: no treatment n=5 and p=0.022, PolySia avDP20 n=3 and p=0.063, LPS n=4, LPS/PolySia avDP20 0.15µM n=3 and p=1.0, LPS/PolySia avDP20 1.5µM n=4 and p=1.0."
NCBI gene IDs:
"SIGLEC11: 114132
VEGFA: 7422"
Figure legend: "D. Analysis of relative VEGFA protein release in human control and SIGLEC11/16 knockout THP1-macrophages. Released protein levels were reduced after 24 hours of co-treatment with LPS (1 µg/ml) and polySia avDP20 (0.15 µM and 1.5 µM) in the human macrophages. Data show mean +/- SEM. * p &lt; 0.05, *** p &lt; 0.001, ANOVA followed by Bonferroni. Statistical analysis was done in relation to the LPS control. WT: no treatment n=9 and p&lt;0.0001, PolySia avDP20 1.5 µM n=6 and p=0.009, LPS n=9, LPS/PolySia avDP20 0.15µM n=6 and p=0.043, LPS/PolySia avDP20 1.5µM n=7 and p=0.0001. Siglec11/16 KO: no treatment n=9 and p=0.349, PolySia avDP20 1.5 µM n= 6 and p=0.249, LPS n=7, LPS/PolySia avDP20 0.15µM n=6 and p=1.0, LPS/PolySia avDP20 1.5µM n=7 and p=1.0."
NCBI gene IDs:
"SIGLEC11: 114132"
Figure legend: "E. Quantification of human control and SIGLEC11/16 knockout THP1-macrophages having ingested cellular debris. PolySia avDP20 (1.5 µM) reduced the percentage of phagocytic cells having ingested drusen-like debris. No response to polySia avDP20 was detectable in the knockout macrophages. Data are presented as mean +/- SEM. n=6. *p ≤ 0.05, *** p ≤ 0.001, ANOVA followed by Bonferroni. Debris treated WT macrophages vs debris plus polySia avDP20 treated WT macrophages p = 0.028, debris plus polySia avDP20 treated WT macrophages vs KO macrophages p = 0.00012."
NCBI gene IDs:
"SIGLEC11: 114132"
Figure legend: "F. Prevention of superoxide release in activated human control and SIGLEC11/16 knockout THP1-macrophages by polySia avDP20. Cultured human THP1-macrophages were stimulated with RPE cell debris or co-stimulated with debris and polySia avDP20. Addition of debris stimulated the production of superoxide. 1.5 µM polySia avDP20 completely prevented the release of superoxide induced by debris challenge. No response to polySia avDP20 was detectable in the knockout macrophages. Data are presented as mean +/- SEM. n=6. p* ≤ 0.05, *** p ≤ 0.001, ANOVA followed by Bonferroni. Untreated WT macrophages vs debris treated WT macrophages p = 0.037, debris treated WT macrophage vs debris plus polySia avDP20 1.5 µM treated WT macrophages p &lt; 0.001, debris plus polySia avDP20 WT macrophages vs KO macrophages p = 0.001,"
NCBI gene IDs:
"SIGLEC11: 114132"
Figure legend: "Transgenic ESZscan4_Emerald cells were sorted and total RNAs were extracted and subjected to RT-qPCR. The graph shows the mRNA expression level of Arg2 in ES subpopulations."
NCBI gene IDs:
"Emerald: 
Arg2: 11847
Zscan4: 665913///100043042///545912"
Figure legend: "(B)Transgenic ESZscan4_Emerald cells were sorted and total proteins were extracted and subjected to immunoblotting. The image is a representative of three independent biological western blotting analysis on arg2 expression in sorted ESZscan4_Emerald and control cells."
NCBI gene IDs:
"Emerald: 
Zscan4: 665913///100043042///545912"
Figure legend: "Transcript (left panel) and protein expression (right panel) levels of Arg2 in ESZscan4_LNGFR cells after transfection with a non-silencing siRNA (siCtrl) or siRNA against Arg2 (siArg2)."
NCBI gene IDs:
"LNGFR: 
Arg2: 11847
Zscan4: 665913///100043042///545912"
Figure legend: "Imaging flow cytometry analysis on Zscan4+ siArg2 and Zscan4+ siCtrl cells. Samples were stained by labelling histone H3 protein. The intensity of H3 fluorescent signal was measured in the nuclear region by creating a mask defined by DAPI staining. The texture feature of fluorescent signal was analysed by using the modulation method (for details see Materials and Methods). Two cell populations (R1 and R2) correspond to two different patterns of fluorescent signal: homogenous and clustered distribution, respectively."
NCBI gene IDs:
"Arg2: 11847
Zscan4: 665913///100043042///545912"
Figure legend: "Electron microscopy images of sorted ESZscan4_Emerald and control cells. Scale bar 1μm"
NCBI gene IDs:
"Emerald: 
Zscan4: 665913///100043042///545912"
Figure legend: "(B) HEK293T  cells transiently expressing FLAG  and FLAG-tagged TEX264  were subjected to immunoprecipitation  (IP). Inputs (20% of the lysates) and immunoprecipitates  (from 80% of the lysates) were analyzed by immunoblotting  using the indicated antibodies."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: "(C) HEK293T  cells transiently expressing WT or mutated TEX264-FLAG  were subjected to immunoprecipitation  with an anti-FLAG  antibody and detected with the indicated antibodies."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: "(D and E) MEFs  stably expressing TEX264-GFP  or its mutant were cultured in starvation  media and immunostained  with an anti-LC3  antibody. Bars: 10 µm and 1 µm (insets) (D). Quantification of the number of TEX264  puncta per cell. Solid bars indicate the medians, boxes the interquartile range (25th to 75th percentile), and whiskers the 0th to 100th percentile. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test. Data were collected from 48 cells for each cell type (E)."
NCBI gene IDs:
"GFP: 
TEX264: 51368"
Figure legend: "(F and G) WT and TEX264-KO HeLa  cells (with or without the indicated TEX264  mutants with a C-terminal FLAG  tag) stably expressing the ER-phagy reporter (ss-RFP-GFP-KDEL) were cultured in the presence of doxycycline  for 24 h to induce the reporter. After doxycycline  was removed, cells were cultured in  starvation  medium  lacking amino acids and serum   for 9 h (F). The band intensities of RFP  and RFP-GFP  were quantified and the ratio of RFP:RFP-GFP  (normalized to WT) is shown. Data represent the mean ± standard error of the mean (SEM) of three independent experiments. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test (G)."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: "(A and B) MEFs stably expressing TEX264-GFP  were cultured in  starvation  media  with or without  bafilomycin A1 (Baf A1) and immunostained  with anti-pTEX264 and anti-LC3  antibodies. Bars: 10 µm and 1 µm (insets) (A). Quantification of the number of pTEX264  puncta per cell. Solid bars indicate the medians, boxes the interquartile range (25th to 75th percentile), and whiskers the 0th to 100th percentile. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test. Data were collected from 35 cells for each cell type (B)."
NCBI gene IDs:
"GFP: 
TEX264: 51368"
Figure legend: "(D, E and F) WT, ATG3-KO, and FIP200-KO HeLa  cells were cultured in  starvation  media  lacking  amino acids and serum  with or without Baf A1  for 1 or 5 h. Cell lysates were analyzed by immunoblotting using the indicated antibodies (D). Relative changes of the ratio of band intensities of phosphorylated TEX264  to HSP90  and total TEX264  during starvation   are shown (E and F). Data represent the mean ± SEM of three independent experiments."
NCBI gene IDs:
"ATG3: 64422
FIP200: 9821"
Figure legend: "(C) HEK293T  cells transiently expressing TEX264-FLAG  and one of the three Halo-CK2A isoforms or Halo-CK2B  were subjected to immunoprecipitation  with an anti-FLAG  antibody and detected with anti-Halo  and anti-FLAG  antibodies."
NCBI gene IDs:
"FLAG: 
Halo: 
CK2A: 283106///1459///1457
CK2B: 1460
TEX264: 51368"
Figure legend: "(D and E) HEK293T  cells transiently expressing WT or TEX264-FLAG  mutants were subjected to immunoprecipitation  (IP). Inputs (20% of the lysates) and immunoprecipitates  (from 80% of the lysates) were analyzed by immunoblotting  using the indicated antibodies (D). The band intensities in the IP  fractions were quantified, and the phospho-serine:FLAG  ratio was calculated. Data represent the mean ± SEM of five independent experiments. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test (E)."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: "(B) HEK293T cells transiently expressing WT or mutated TEX264-FLAG  were subjected to immunoprecipitation  with an anti-FLAG  antibody and detected with the indicated antibodies."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: "(C and D) MEFs  stably expressing TEX264-GFP  or its mutants were cultured in starvation  media and immunostained  with an anti-LC3  antibody. Bars: 10 µm and 5 µm (insets) (C). The intensity of the GFP  signal under starvation  conditions was quantified at TEX264-GFP puncta and the ER. The puncta:ER signal ratio was calculated. Solid bars indicate the medians, boxes the interquartile range (25th to 75th percentile), and whiskers the 0th to 100th percentile. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test. Data were collected from 70 puncta for each cell type (D)."
NCBI gene IDs:
"GFP: 
TEX264: 51368"
Figure legend: "(E and F) WT and TEX264-KO (expressing WT or TEX264-FLAG  mutants) HeLa  cells stably expressing the ER-phagy reporter were cultured in the presence of doxycycline  for 24 h to induce the reporter. After doxycycline  was removed, the cells were cultured in starvation  medium lacking amino acids and serum  for 9 h (E). The band intensities of RFP and RFP-GFP  were quantified and the ratio of RFP:RFP-GFP  (normalized to WT) is shown. Data represent the mean ± SEM of four independent experiments. Differences were statistically analyzed by one-way ANOVA and Tukey's multiple comparison test (F)."
NCBI gene IDs:
"FLAG: 
TEX264: 51368"
Figure legend: " C RNAlow neoblast size in intact animals and at 48 hpa in control or TOR KD animals (n ≥ 55). "
NCBI gene IDs:
"TOR: "
Figure legend: " D Quantification of H3P immunostaining in intact animals and at 48 hpa in control or TOR KD animals (n = 5-10). "
NCBI gene IDs:
"TOR: "
Figure legend: " E RNAlow neoblast size in intact animals and at 48 hpa in control, raptor or rictor KD animals (n ≥ 54). "
NCBI gene IDs:
"raptor: 
rictor: "
Figure legend: " F FACS plots of Hoechst and Pyronin Y stained cells isolated from intact and regenerating animals following 1 day of nocodazole treatment in control or TOR KD animals. Percentages indicate the proportion of events within each gate. "
NCBI gene IDs:
"TOR: "
Figure legend: "A Representative WISH images for Lrig-1."
NCBI gene IDs:
"Lrig-1: "
Figure legend: " B RNAlow neoblast size in intact animals following control or Lrig-1 knockdown (n ≥ 53 technical replicates). "
NCBI gene IDs:
"Lrig-1: "
Figure legend: " C Quantification of H3P immunostaining in intact animals following control or Lrig-1 knockdown (n = 9-10 technical replicates). "
NCBI gene IDs:
"Lrig-1: "
Figure legend: " D Quantification of H3P immunostaining at homeostasis (fixed at 7 days post-RNAi, white region) and at 48 hpa (amputated at 5 days post-RNAi and fixed at 7 days post-RNAi, yellow region) during the first round of regeneration in control or Lrig-1 KD animals (n = 10-17). "
NCBI gene IDs:
"Lrig-1: "
Figure legend: " E Quantification of unpigmented blastema area (as % total tissue area) in regenerating control or Lrig-1 KD animals from 2 dpa to 7 dpa during the first round of regeneration (n = 10 animals per condition per time point). The same animals were used for all timepoints. Representative images from 7 dpa are shown. Scale bars, 500 µm. "
NCBI gene IDs:
"Lrig-1: "
Figure legend: " F Quantification of H3P immunostaining at homeostasis (fixed at 14 days post-RNAi, white region) and at 48 hpa (amputated at 12 days post-RNAi and fixed at 14 days post-RNAi, yellow region) during a second round of regeneration in control or Lrig-1 KD animals (n = 10-18). "
NCBI gene IDs:
"Lrig-1: "
Figure legend: " G Quantification of unpigmented blastema area (as % total tissue area) in regenerating control or Lrig-1 KD animals from 2 dpa to 7 dpa during the second round of regeneration (n = 9-10 animals per condition per time point). The same animals were used for all timepoints. Representative images from 7 dpa are shown. Scale bars, 500 µm. "
NCBI gene IDs:
"Lrig-1: "
Figure legend: "(A-D) EM images of cortical neuropil show an absence of AVs and normal neurite profile in 9-mo-old NTg mouse brains (A, arrowheads outline normal neurites) and a marked accumulation of AVs within enlarged or dystrophic neurites in PS1/APP mice (B, arrowheads outline dystrophic neurite profiles in C) and biopsied brain material from an AD patient (B, inset). At higher magnification (C), AVs include autophagosomes (arrows) and multilamellar bodies (arrowhead). In normal dendrites of PS1/APP mice, multiple AVs are frequently seen (D, arrows)."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "(E and F) LC3 quantification analyzed from immunoblots of LC3-I and LC3-II (top) in prefrontal cortical homogenates from cases of nonaffected (Cont), early stage (preclinical) AD (AD-ES), and moderate AD (AD-MS; E), and from brains of 18-22-mo-old PS1/APP (PA) mice (n = 3; F) compared with nontransgenic (NTg) controls (n = 3; *, P &lt; 0.01). Error bars represent SEM."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "(G-L) LC3 immunofluorescence in 9-mo-old PS1/APP mice can be seen mainly as puncta in dystrophic dendrites of the cortex (G, arrows) and along adjacent dendrites. LC3 (H, arrows) is strong in dystrophic neurites in the periphery (asterisks) of a thioflavin S-labeled plaque core (H, inset) but is less so in neurites closest (H, arrowheads) to the Aβ deposit. LC3 is diffuse and uniform in neurons of NTg mice (I and J) but is predominantly vesicular and distributed more to the dendrites (arrows) than the cell soma (arrowheads) in 9-mo-old PS1/APP cortex (K and L)."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "Ultrastructural inspection of brain tissue from PS1/APP mice (A-D) shows that AVs (A and B, arrows) are five times more frequent in the dendrites of 8-wk-old PS1/APP than in those of age-matched NTg mice. The frequency of AVs per EM field (C) and mean number of AVs per EM field (D) within the hippocampal molecular layer (n = 3) are shown."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "LC3immunoblot and analysis (E) and immunofluorescent labeling (F-K) of the hippocampaldendrites (brackets) in 8-9-wk-old PS1/APP and NTg mice show LC3-II elevation (P &lt; 0.05) in 8-wk-old PS1/APP compared with NTg mice (E). (D) *, P &lt; 0.001. (E) *, P &lt; 0.05. Error bars represent SEM. LC3 immunoreactivity in pyramidal celldendrites is increased in 9-mo-old (F-H) and 9-wk-old (I-K) PS1/APPmice and frequently exhibits a punctate labeling pattern, which is more evident at 9 mo than at 9 wk (H and K, arrows) and is uncommon in NTg mice (F and I). Bars (F, G, I, and J), 20 μm; (H and K), 10 μm."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "Immunolocalization of PS1 in plaques and AVs within dystrophic neurites in AD and PS1/APP mice. Cingulate cortex from 9-mo-old PS1/APP mice immunolabeled with PS1 antibody and NT1 showed that PS1 localized to plaques (A). At higher magnification, anti-PS1 antibodies strongly labeled neuritic profiles that were distributed within the plaque corona (B)."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "PS1 immunoreactivity is identified by IEM in AVs within dystrophic neurites of PS1/APP animals (C and D)Arrowheads identify tubulovesicular membrane labeling. PS1 (C-F, arrows) was localized on the outer limiting membrane of the AV but not in mitochondria (Mito) or on plasma membranes (PM)."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "Induction of macroautophagy in L/APP, SH-SY5Y, and N2a cells. (A and B) EM images showing changes in the number of AVs (arrows) in L/APP-overexpressing APP695 (L/APP cells) grown in complete medium (A, top left) or in medium lacking Leu and His (A, top right) for 6 h and in SH-SY5Y cells grown in the presence (A, bottom left) or absence (A, bottom right) of serum. At higher magnification, early and late AVs with typical morphologies are seen in a Leu/His-deprived L/APP cell (B)."
NCBI gene IDs:
"APP: 249"
Figure legend: "(D) Western blots confirm the cytochemical evidence for increased LC3-II levels as well as phospho-mTOR (P-2481) but not total mTOR after macroautophagy induction by Leu and His deprivation or 10 nM rapamycin (Rap) and macroautophagy inhibition by 5 mM 3MA in L/APP, N2a, and SH-SY5Y cells. Immunoblots for LC3 in SH-SY5Y cells and P-2481 mTOR in L/APP cells have been spliced but are derived from the same blot."
NCBI gene IDs:
"APP: 249"
Figure legend: "Immunolocalization of Aβ in AVs from L/APP cells and PS1/APPbrains and γ-secretase components (PS1 and nicastrin) in L/APP cells. Immunogold localization of Aβ40 (A), Aβ42 (B), PS1 (C), nicastrin (D), and in the absence of primary antibody (E) in L/APP cells grown for 6 h in the absence of Leu and His. (F) Quantification of gold particle frequency in AV or tubulovesicular compartments (TBV), which comprise 27.0 ± 11.0 and 19.2 ± 7.2%, respectively, of the total cell area. Error bars represent SEM."
NCBI gene IDs:
"APP: 249"
Figure legend: "(G and H) IEM followed by silver stain enhancement for Aβ40 was performed in 9-mo-old PS1/APP mice."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "(A and B) Ultrastructure of AVs (A1 and A2) and lysosomes in subcellular fractions from serum-deprived L/APP cells (A) and Western blot analysis (B) for LC3-II, rab24, APP, βCTF, PS1 (PS1 amino-terminal fragment), and nicastrin (NCT) in L/APP subcellular fractions. AVs, A1 and A2; L, lysosomes; E, tubulovesicular compartments (Golgi/ER/endosomes); P, postnuclear pellet; C, cytosol; M, mitochondria. Empty lanes in the original blot have been removed from the figure and noted with a white line."
NCBI gene IDs:
"APP: 249"
Figure legend: "(G) γ-Secretase activity in AV and lysosome fractions from mice blastocysts in which the PS1 and PS2 genes were deleted (PS KO; BD8) or in which human PS1 was stably transfected into the PS KO blastocysts (hPS1; BD8/hPS1). Numbers on x axis are in minutes."
NCBI gene IDs:
"hPS1: 5663
PS1: 19164
PS2: 19165"
Figure legend: "C) Immunofluorescence image of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP targeted cells stained with antibodies against CENP-T. Images in C maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "E) Quantification of CENP-T signal intensity at MUC4 ectopic foci vs. endogenous centromeres, normalised to CENP-T signal intensity at endogenous centromeres (=1, red line). LinesLines = Mean ± SD, 3 experiments, each with ≥ 10 metaphases analysed per condition. Each point = 1 focus or centromere. ns = p&gt;0.05, *** = p&lt;0.01, **** = p&lt;0.001 (Kruskal Wallis test with Dunn's multiple comparison correction)."
NCBI gene IDs:
"MUC4: 4585"
Figure legend: "H) Quantification of KNL‑1 signal intensity at MUC4 foci, normalised to KNL‑1 signal intensity at centromeres (=1, red line). LinesLines = Mean ± SD, 3 experiments, each with ≥ 10 metaphases analysed per condition. Each point = 1 focus or centromere. ns = p&gt;0.05, *** = p&lt;0.01, **** = p&lt;0.001 (Kruskal Wallis test with Dunn's multiple comparison correction)."
NCBI gene IDs:
"MUC4: 4585"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. C) Immunofluorescence images of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP targeted cells stained with antibodies against CENP-T. Images in are maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. F) Immunofluorescence images of CENP‑T∆C‑dCas9‑EGFP targeted cells stained with antibodies against KNL-1. Images in ,F are maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. I) Immunofluorescence images of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP targeted cells stained with antibodies against Ndc80. Images in I) are maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. A) Immunofluorescence images of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP targeted metaphase cells stained with antibodies against alpha-tubulin. B) Immunofluorescence images of mitotic cells ­­­with CENP‑T∆C‑dCas9‑EGFP targeting after nocodazole treatment. C) Length measurements of individual EGFP foci in µm. Example measurements are indicated on zooms in A-B) in orange. Data is ≥20 cells per condition, from 1 experiment. Lines = Mean ± SEM. * = p&lt;0.05, ** = p&lt;0.01,**** = p&lt;0.001 (Kruskal-Wallis with multiple comparison correction). Images in A,B are maximum intensity projections taken across the depth of the EGFP foci. In A,B) Orange bars = measured lengths in C."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. D) Immunofluorescence images of metaphase cells with CENP-T∆C-dCas9‑EGFP targeting after cold treatment, stained with antibodies against alpha-tubulin. E) Percentage of metaphase cells showing microtubule (MT) recruitment. ≥50 metaphases per condition, from 1 experiment.. are maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP-T: 80152
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN in HEK293T cells. F) Frames from live cell imaging of cells with dCas9 targeted to Chr9-CEN, or an EGFP negative cell. are maximum intensity projections taken across the depth of the EGFP foci."
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"
Figure legend: "Targeting of dCas9-EGFP or CENP‑T∆C‑dCas9‑EGFP to Chr9-CEN and Chr1‑TELO in HEK293T cells. A) Immunofluorescence images of paired EGFP foci for each attachment status category. Images were chosen where both the EGFP foci and any of their attachments lay in the same Z-plane for clarity of presentation. B) Percentage of paired EGFP foci showing each attachment status. are from ≥75 EGFP foci pairs from n≥15 metaphases per condition 1 experiment for"
NCBI gene IDs:
"EGFP: 
Cas9: 69900935
CENP‑T: 80152"