EMBO/SourceData huggingface dataset:
Figure legend: "B A plasmid encoding the truncated mutant composed of Smad6 amino acids 422-441 (Myc-Smad6(422-441)) or wild-type Smad6 MH2 domain (Myc-Smad6-MH2) was co-transfected with the HA-tagged full-length Pellino-1 plasmid into HEK293 cells. Cell lysates were immunoprecipitated (IP) with anti-Myc or anti-HA antibody, and immunoblotted (IB) with anti-HA or anti-Myc antibody, respectively. The vector, CS3MTBXA-6xMyc, was transfected as a negative control. IP, immunoprecipitation; IB, immunoblot; TCL, total cell lysates. Data are representative of at least three independent experiments."
NCBI gene IDs:
"Smad6: 4091"
Figure legend: "C The Myc-Smad6(422-441) plasmid was co-transfected with full-length Flag-IRAK1, Flag-TRAF6, Flag-MyD88, or HA-Pellino-1 plasmid into HEK293 cells. Cell lysates were immunoprecipitated with the indicated antibodies and immunoblotted with anti-Myc antibody. IgG was added as a negative control for IP. Data are representative of at least three independent experiments."
NCBI gene IDs:
"IRAK1: 3654
MyD88: 4615
Pellino-1: 57162
TRAF6: 7189"
Figure legend: "D, E The SBE-Luc or 5xNF-κB-Luc reporter plasmids were co-transfected with an empty vector or the Myc-Smad6(422-441) plasmid into CMT-93 cells, respectively. After 24 h, cells were treated with TGF-β1 for 6 h or LPS for 2 h, and luciferase activities were measured and normalized."
NCBI gene IDs:
"luciferase: 
Smad6: 4091"
Figure legend: "F The BRE-Luc reporter plasmid was co-transfected with an empty vector or the Myc-Smad6(422-441) plasmid or full-length Smad6 into RAW264.7 cells, respectively. After 24 h, cells were treated with BMP4 for 6 h and luciferase activity was measured and normalized."
NCBI gene IDs:
"luciferase: 
Smad6: 4091
Smad6: 17130"
Figure legend: "G A plasmid encoding the truncated mutant composed of Smad6 amino acids 422-441 (Myc-Smad6(422-441)) or wild-type Smad6 MH2 domain (Myc-Smad6-MH2) or full-length Smad6 (Myc-Smad6) was co-transfected with HA-tagged full-length Smad4 or HA-tagged full-length Pellino-1 plasmid into HEK293 cells, respectively. Cell lysates were immunoprecipitated (IP) with anti-Myc and immunoblotted (IB) with anti-HA or anti-Myc antibody, respectively. The vector, pCS3MTBXA-6xMyc, was co-transfected with HA-Smad4 or HA-Pellino-1 as a negative control. Data are representative of at least three independent experiments."
NCBI gene IDs:
"Smad6: 4091"
Figure legend: "A Pre-treatment of Smaducin-6 reduces LPS-induced interleukin-6 (Il6) gene expression in a dose-dependent manner in RAW264.7 cells. Il6 gene expression was analyzed by qRT-PCR."
NCBI gene IDs:
"Il6: 16193
interleukin-6: 16193"
Figure legend: "B, C Pre-treatment of 100 nM Smaducin-6 or scrambled peptide (Pal-Scram #1) for 30 min inhibits (B) NF-κB-mediated luciferase gene expression when RAW264.7 cells are treated with LPS for 2 h. Luciferase activity in (B) was normalized to β-galactosidase activity."
NCBI gene IDs:
"luciferase: 
β-galactosidase: "
Figure legend: "D Peli1 knockdown or wild-type humanTHP1 cells were pre-treated with 100 nM Pal-Scram peptide and Smaducin-6 for 30 min and subsequently treated with LPS for 2 h. Il6 and Peli1 gene expression were analyzed by qRT-PCR. Data show the mean ± SD of three independent experiments."
NCBI gene IDs:
"Il6: 3569
Peli1: 57162"
Figure legend: "(A) Relative mRNA expression of Foxc1 and Foxc2 in isolated blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) from Foxc1fl/fl;Foxc2fl/fl mouse distal jejuna in sham and I/R-4h groups. Isolated epithelial cells (Epis) were used as a relative control, Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 5~9, *P&lt;0.05, **P&lt;0.01, n.s. = not significant. The box-and-whisker plots in (A) display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc1: 17300
Foxc2: 14234"
Figure legend: "(B-C) Representative confocal images of the whole-mount intestine stained with CD31/LYVE1 (B), FOXC1/CD31/LYVE1 (C, left) and FOXC1/LYVE1 (C, right) in Foxc1fl/fl;Foxc2fl/fl mice. Images of maximum intensity projections (B) show the intestinal blood (green) and lymphatic (white) vasculatures from villous to submucosa. The regions in pink box 1~8 in Figure 1B are chosen for the specific images in Figure 1C. Scale bars = 50 μm. (C) Images of optical sections with high magnification chosen from specific regions shown in Figure 1B (B1~B8), with an additional channel of FOXC1 staining (red), show the up-regulation of FOXC1 in BECs (white arrows in blood vessels) and LECs (violet arrows in lymphatic vessels) at the level of villus and submucosa 4 h after I/R. Scale bars = 20 μm."
NCBI gene IDs:
"Foxc1: 17300
Foxc2: 14234"
Figure legend: "(F) Relative mRNA expression  of Foxc1  and Foxc2  in isolated CD45-CD31+ ECs  from distal jejuna 12d after Tm  treatment. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 4~5, *P&lt;0.05. Data information: The box-and-whisker plots in (F) display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc1: 17300
Foxc2: 14234"
Figure legend: "(C-E) Quantification of Chiu Score for Control and EC-Foxc-DKO (C), Control and EC-Foxc1-KO (D), and Control and EC-Foxc2-KO groups (E) at I/R-24h. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 3 in C sham groups, N = 7~12 in C, D, E I/R-24h groups, *P&lt;0.05, ****P&lt;0.0001. The box-and-whisker plots in display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc1: 17300
Foxc2: 14234
Foxc: 14234///17300"
Figure legend: "(I) Representative images of intestinal crypts immunostained with β-catenin and the intestinal epithelial stem cell (ISC) marker OLFM4. At I/R-48h, the nuclear translocation of β-catenin in ISCs (dotted circles) was found in the control, whereas it was seldom found in EC-Foxc-DKO mice. Paraffin sections (4 µm), (J, K) (J) Quantification of relative fluorescent intensity (FI) of β-catenin immunostaining within ISC and (K) quantification of number of OLFM4+ ISCs per crypt were performed based on Figure 2I. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 4~5, *P&lt;0.05, n.s. = not significant. The box-and-whisker plots in display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "(L) Representative images of intestinal mucosa labeled with Cyclin D1 (CCND1) in EC-Foxc-DKO mice compared with Control group in sham, 24h and 48h after I/R. Paraffin sections (4 µm), (M) Quantification of the number of CCND1+ epithelial cells per crypt based on Figure 2L. Data are box-and-whisker plots, Kruskal-Wallis One-way ANOVA test, each symbol represents one mouse, N = 4~6, **P&lt;0.01, ****P&lt;0.0001, n.s.=not significant. The box-and-whisker plots in display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "Chiu Score analysis from H&amp;E stained distal jejunum 24h after I/R for Control (Foxc1f/f;Foxc2f/f) and LEC-Foxc-DKO (Vegfr3-CreERT2;Foxc1f/f;Foxc2f/f) mice (B), Control (Foxc1f/f) and LEC-Foxc1-KO (Vegfr3-CreERT2;Foxc1f/f) mice (C) Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N=9~13, *P&lt;0.05, **P&lt;0.01. display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Cre: 2777477
Vegfr3: 14257
Foxc1: 17300
Foxc2: 14234
Foxc: 14234///17300
ERT2: 26417"
Figure legend: "(F) Representative images of crypts immunostained with OLFM4 and β-catenin in control and LEC-Foxc-DKO mice 24h after I/R. The accumulation of β-catenin in the nuclei of ISCs (dotted circles) was found in Control mice but inhibited in LEC-Foxc-DKO mice. Paraffin sections (4 µm), scale bars = 20 μm."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "(A) Representative images of intestinal whole-mount VEGFR2/CD31 (green/red) immunostaining show increased VEGFR2 (green) expression at the angiogenic front of villous blood capillaries in control mice at I/R-18.5h. In EC-Foxc-DKO mice, the increase of VEGFR2 is inhibited in villous blood vessels and the blood vasculatures are damaged (arrow). (B) Representative images of intestinal whole-mount VEGFR3/LYVE1 (red/green) immunostaining. 18.5h after I/R, VEGFR3 (red) is increased in lacteals especially at the lacteal tips in the control but is inhibited in the EC-Foxc-DKO lacteals (arrow)."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "(D) Violin plots of the Rspo3 expression in trophocytes and LECs in intestine at I/R-18.5h. Mann-Whitney U test, each symbol represents one cell; N = 12 and 12 in control and EC-Foxc-DKO trophocytes, respectively; N=16 and 5 in control and EC-Foxc-DKO LECs."
NCBI gene IDs:
"Foxc: 14234///17300
Rspo3: 72780"
Figure legend: "(E) Validation study by qPCR for the detection of relative mRNA expression of Rspo3 in the sorted intestinal LECs at I/R-18.5h. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 5~6, **P&lt;0.01. The box-and-whisker plots display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Rspo3: 72780"
Figure legend: "(D) Violin plots of the Cxcl12 expression in fibroblasts and telocytes/trophocytes. Mann-Whitney U test, each symbol represents one cell, N = 67 and 98 in control and EC-Foxc-DKO mice, respectively; n.s. = not significant."
NCBI gene IDs:
"Cxcl12: 20315
Foxc: 14234///17300"
Figure legend: "(F) Relative mRNA expression of Cxcl12 in Dynabeads-isolated ECs (CD45-CD31+) from distal jejunum. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 6~10, *P&lt;0.05. The box-and-whisker plots display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Cxcl12: 20315"
Figure legend: "FOXC1 and FOXC2 co-occupy as well as the ECRs and promoters of CXCL12 in HUVECs (D). Quantitative-ChIP assay was performed using rabbit (R) anti-FOXC1 and mouse (M) anti-FOXC2 antibodies to analyze the recruitment of FOXC on promoters and/or ECRs in HUVECs respectively. Values were quantified against IgG controls. Data are mean ± SEM, paired t-test, each symbol represents data collected from one experiment, N = 4, * P&lt;0.05, ** P&lt;0.01, n.s.=not significant."
NCBI gene IDs:
"CXCL12: 6387"
Figure legend: "In RSPO3 rescue experiment, each mouse was treated with 5 μg RSPO3 in 100μL PBS by retro-orbital injection 30 min before ischemia. PBS treated mice were used as vehicle control. (C) Representative images of crypts immunostained with OLFM4 and β-catenin in PBS/RSPO3 treated EC-Foxc-DKO mice 24h after I/R. The accumulation of β-catenin in the nuclei of ISCs (dotted circles) was found in RSPO3 rescued mice. Paraffin sections (4 µm), (D) Quantification of relative fluorescent intensity (FI) of β-catenin immunostaining within ISC and (E) quantification of the number of OLFM4+ ISCs were performed based on Figure 9C. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 4~6, *P&lt;0.05."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "In RSPO3 rescue experiment, each mouse was treated with 5 μg RSPO3 in 100μL PBS by retro-orbital injection 30 min before ischemia. PBS treated mice were used as vehicle control. (F) Representative images of immunostaining of CCND1 in intestines in PBS/RSPO3 treated EC-Foxc-DKO mice at I/R-24h. Scale bars = 100 µm. (G) Quantification of the number of CCND1+ epithelial cells per crypt at I/R-24h are based on Figure 9F. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 7, **P&lt;0.01."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "In RSPO3 rescue experiment, each mouse was treated with 5 μg RSPO3 in 100μL PBS by retro-orbital injection 30 min before ischemia. PBS treated mice were used as vehicle control. (H) Relative mRNA expression of Rspo3 in sorted LECs and Cxcl12 in sorted BECs from intestines of PBS/RSPO3 treated EC-Foxc-DKO mice at I/R-18.5h. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 4~6, *P&lt;0.05."
NCBI gene IDs:
"Cxcl12: 20315
Foxc: 14234///17300
Rspo3: 72780"
Figure legend: "In RSPO3 rescue experiment, each mouse was treated with 5 μg RSPO3 in 100μL PBS by retro-orbital injection 30 min before ischemia. PBS treated mice were used as vehicle control. Representative images of H&amp;E staining show the rescue effects of RSPO3 in intestinal mucosa LEC-Foxc-DKO (I) mice as well as their control mice 24h after I/R. Red numbers indicate the Chiu scores. Quantification of Chiu Score for the intestines at I/R-24h are based H&amp;E staining as shown in Figure 9A and 9I. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 6~13, **P&lt;0.01."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "In RSPO3 rescue experiment, each mouse was treated with 5 μg RSPO3 in 100μL PBS by retro-orbital injection 30 min before ischemia. PBS treated mice were used as vehicle control. (K) Representative images of CD31/LYVE1 immunostaining in the intestines of PBS/RSPO3 treated LEC-Foxc-DKO mice at I/R-24h. (L, M) Quantification of the vessel density (L) (= vessel area/total intestinal tissue area x 100%) for the blood (B) and/or lymphatic (L) vessels (markers listed below the graph were used to identify B and L) as well as the measurement of lacteal length (M) was performed based on Figure 9K. The Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 7, **P&lt;0.01, n.s.=not significant."
NCBI gene IDs:
"Foxc: 14234///17300"
Figure legend: "In CXCL12 rescue experiments, mice were treated with 50μg/kg CXCL12 in PBS by retro-orbital injection 30 min before ischemia. Mice treated with PBS were used as control. (H) Representative confocal images of CD31 immunostaining of distal jejuna in PBS- and CXCL12- treated EC-Foxc-DKO mice after I/R at 24h. Paraffin sections (15 µm), scale bars = 100 μm. L represents the lacteal length measured in Figure 10J. (I, J) (I) Quantification of CD31+ vessel density (% = total CD31+ vessel area/total intestinal tissue area x 100%) and (J) quantification of lacteal length were performed based on Figure 10H. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 4~6, **P&lt;0.01."
NCBI gene IDs:
"Foxc: 17300///14234"
Figure legend: "In CXCL12 rescue experiments, mice were treated with 50μg/kg CXCL12 in PBS by retro-orbital injection 30 min before ischemia. Mice treated with PBS were used as control. (K) Relative mRNA expression of Rspo3 in sorted intestinal LECs from PBS/CXCL12 treated EC-Foxc-DKO mice at I/R-18.5h. Data are box-and-whisker plots, Mann-Whitney U test, each symbol represents one mouse, N = 5~6, n.s.=not significant. The box-and-whisker plots in display the median value (central band in the box), second and third quartiles (bottom and top ends of the box, respectively) as well as minimum/maximum values (whiskers blow/above the box) of the data sets."
NCBI gene IDs:
"Foxc: 17300///14234
Rspo3: 72780"
Figure legend: "Western blot analysis of total brain lysates of wild type, Tmem106b-/-, Grn-/- and Grn-/-/Tmem106b-/- mice using antibody 6F2 to detect TMEM106B. (4.5-month-old mice with the given genotype; n = 3 biological replicates per genotype)."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Hind limb clasping test wild type, Tmem106b-/-, Grn-/-, Grn+/-/Tmem106b-/- and Grn-/-/Tmem106b-/- mice. Representative pictures are shown of 3 mice analyzed for each genotype at 3 months of age."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Rotarod performance of wild type, Tmem106b-/-, Grn-/-, Grn+/- /Tmem106b-/- and double knockout animals at 3 to 4 months of age. Number of animals used for analysis, 10 wild type, 12 Tmem106b-/-, 5 Grn-/-, 7 Grn+/-/Tmem106b-/-, 3 Grn-/-/Tmem106b-/- mice."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Representative picture of a 4.5-month-old double knockout (Grn-/-/Tmem106b-/- ) mouse after manual flip."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Volcano plot of differentially expressed brain mRNAs of 4.5-month-old mice (n = 3 biological replicates per genotype) detected by the Neuropathology panel of NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for all volcano plots. Gliosis and Myelination related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (A) Comparison of differentially expressed brain mRNAs from Tmem106b-/- and wild type mice. From 680 detected genes 21 are significantly upregulated, while 10 are significantly reduced."
NCBI gene IDs:
"Tmem106b: 71900"
Figure legend: "Volcano plot of differentially expressed brain mRNAs of 4.5-month-old mice (n = 3 biological replicates per genotype) detected by the Neuropathology panel of NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for all volcano plots. Gliosis and Myelination related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (B) Comparison of Grn-/- and wild type mice. From 680 detected genes 12 are significantly upregulated, while 6 are significantly reduced."
NCBI gene IDs:
"Grn: 14824"
Figure legend: "Volcano plot of differentially expressed brain mRNAs of 4.5-month-old mice (n = 3 biological replicates per genotype) detected by the Neuropathology panel of NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for all volcano plots. Gliosis and Myelination related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (C) Volcano plot for Grn-/-/Tmem106b-/- and wild type mice. From 680 detected genes 79 are significantly upregulated, while 24 are significantly reduced."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "D. Display of differently expressed genes overlapping between the analyzed genotypes from (A-C). Overlap was considered if a gene was significantly changed in the respective mouse models with more than 20% change in at least one mouse model. Note that 9 genes are significantly altered in all 3 genotypes in comparison to the wild type. 7 genes are exclusively overlapping between the Grn-/- and the Grn-/-/Tmem106b-/- mice. Tmem106b-/- and Grn-/-/Tmem106b-/- mice share 15 significantly altered genes. Tmem106b-/- and Grn-/- mice share one significant altered gene. Data represent the mean ± SD."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Analysis of brain mRNA expression of 4.5-month-old mice (5 wild type 4 Tmem106b-/- mice) detected by the Neuroinflammation panel by NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for in all volcano plots. Gliosis related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (A) Comparison of differentially expressed mRNA of Tmem106b-/- mice in comparison to wild type mice. From 622 detected genes 21 are significantly upregulated, while 12 are significantly reduced."
NCBI gene IDs:
"Tmem106b: 71900"
Figure legend: "Analysis of brain mRNA expression of 4.5-month-old mice (5 wild type, 4 Grn-/ mice) detected by the Neuroinflammation panel by NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for in all volcano plots. Gliosis related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (B) Comparison of differentially expressed mRNA in Grn-/- and wild type mice. From 622 detected genes 32 are significantly upregulated, while only 1 is significantly reduced."
NCBI gene IDs:
"Grn: 14824"
Figure legend: "Analysis of brain mRNA expression of 4.5-month-old mice (5 wild type 5 Grn-/-/Tmem106b-/- mice) detected by the Neuroinflammation panel by NanoString. Expression changes were only taken into consideration when the extent of change was above 20% as indicated by vertical lines for in all volcano plots. Gliosis related genes are highlighted if changes are above 20%. Fold changes are displayed after log2 transformation. (C) Volcano plot of differentially expressed brain mRNAs from Grn-/-/Tmem106b-/- mice in comparison to wild type mice. From 622 detected genes 110 are significantly upregulated, while 16 are significantly reduced."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "D. Display of differently expressed genes overlapping between the analyzed genotypes from (A-C). Overlap was considered if a gene was significantly changed in the respective mouse models with more than 20% change in at least one mouse model. Note that 19 genes are significantly altered in all 3 genotypes in comparison to the wild type. 17 genes are exclusively overlapping between the Grn-/- and the Grn-/-/Tmem106b-/- mice. Tmem106b-/- and Grn-/-/Tmem106b-/- mice share 17 significantly altered genes. Data represent the mean ± SD."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "In vivo 18F-GE180 TSPO μPET imaging. Two female Grn-/-/Tmem106b-/- and five female wild type mice underwent longitudinal TSPO μPET at 2.0 and 3.5 months of age. All analyses were performed by PMOD (V3.5, PMOD technologies). Normalization of injected activity was performed by the previously validated myocardium correction method."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "Representative confocal images of control (CTL-1) and OPA1S545R patient fibroblasts treated with OPA1, DNM1L, or non-targeting (NT) siRNAs for 72 hours and imaged as described in A. Scale bar=20μm. Passage number between P12-14. Mitochondrial morphology quantification of D. Data represent mean ± SD of three independent experiments, (3219-5857 cells per cell line), One-way ANOVA; ****p &lt; 0.0001, ns; not significant."
NCBI gene IDs:
"DNM1L: 10059
OPA1: 4976"
Figure legend: "Representative confocal images of control (CTL-1) and OPA1S545R patient fibroblasts treated with 50μM cycloheximide (CHX) where indicated for 6 hours. Imaging as described in A. Scale bar=20μm. Passage number between P14-P15. Mitochondrial morphology quantification of F. Data represent mean ± SD of two independent experiments, (879-4154 cells per cell line), One-way ANOVA; ****p &lt; 0.0001, ns; not significant."
NCBI gene IDs:
"OPA1: 4976"