EMBO/SourceData huggingface dataset:
Figure legend: "(A) Viral DNA polymerase inhibitor  phosphonoacetic acid (PAA, 300 μg/ml) treatment suppressed Gadd45b mRNA expression. Latently-infected SCG neurons were treated with LY294002 for 18 hrs to induce reactivation then treated with or without PAA  until the termination time-points  indicated. RNA was then collected and levels of Gadd45b mRNA quantified by RT-qPCR (n=3 biological replicates, the bars and error bars are mean ± SD). Data information: P values equal to or less than 0.05 were considered significant, asterisks denote statistical significance (*, p&lt;0.05; ***, p&lt;0.001; ****, p&lt;0.0001). P values are calculated using two-tailed unpaired Student's t test."
NCBI gene IDs:
"Gadd45b: 299626"
Figure legend: "(G) Latent SCG neurons  treated with  LY294002 and WAY-150138 for 72 hrs, the Gadd45b nuclear  puncta-presenting neurons  were ICP4  negative (none observed in over 60   Gadd45b nuclear  puncta-positive  neurons), but a small population (~5%) in Gadd45b nuclear-enriched neurons were  ICP4 positive. Scale bar, 20 μm."
NCBI gene IDs:
"ICP4: 2703392"
Figure legend: "(A) Depletion of  Gadd45b  expression using lentiviral-transduced shRNAs in latently-infected  SCG neurons  case cause HSV-1  reactivation. HSV1-GFP-Us11 latently-infected cultures were infected with lentiviruses expressing either two different shRNAs against Gadd45b mRNA or a non-silencing shRNA (NS). Reactivation was quantified by scoring the percentage of wells expressing GFP  after 5 days. Knockdown efficiencies for individual shRNAs in latently-infected SCG neurons were confirmed by RT-qPCR using RNA collected in parallel. Reactivation rates were quantified from 30 wells for each condition, 3 biological replicates. RT-qPCR were quantified from 3 biological replicates. The bars and error bars are mean ± SD. Data information: P values equal to or less than 0.05 were considered significant, asterisks denote statistical significance (*, p&lt;0.05; ***, p&lt;0.001; ****, p&lt;0.0001). P values are calculated using two-tailed unpaired Student's t test. P values &gt; 0.05 were not significant (ns)."
NCBI gene IDs:
"Gadd45b: 299626"
Figure legend: "(B) smFISH shows that depletion of Gadd45b leads to increased UL36 expression. smFISH was performed 3 days post-lentiviral transduction. smFISH  signals were quantified and plotted using ImageJ and Prism. Left: 3 biological replicates, 30 cells were quantified for each replicate. The horizonal lines are mean. Right: 3 biological replicates. The horizonal lines and error bars are mean ± SD."
NCBI gene IDs:
"Gadd45b: 299626
UL36: 2703357"
Figure legend: "(C) Ectopic expression of Gadd45b-Myc-Flag  inhibits viral mRNA  expression. Lentivirus-delivered ectopic expression of Gadd45b-Myc-Flag in SCG neurons  was confirmed by immunoblotting. Latently-infected SCG neurons  were transduced with Gadd45b-Myc-Flag for 3 days and then treated with LY294002 and WAY-150138 to induce reactivation. RNA was collected at the indicated time-points (LY treatment for 18 or 72 hours) and levels of viral  UL36 mRNA quantified by RT-qPCR. 3 biological replicates. The bars and error bars are mean ± SD. Data information: P values equal to or less than 0.05 were considered significant, asterisks denote statistical significance (*, p&lt;0.05; ***, p&lt;0.001; ****, p&lt;0.0001). P values are calculated using two-tailed unpaired Student's t test. P values &gt; 0.05 were not significant (ns)."
NCBI gene IDs:
"Flag: 
Myc: 
Gadd45b: 299626
UL36: 2703357"
Figure legend: "(D) Ectopic expression of Gadd45b-Myc-Flag antagonizes LY294002-induced HSV-1 reactivation. Latently-infected SCG neurons were either transduced with vector control (Ctrl) or Gadd45b-Myc-Flag  from  lentivirus for 3 days prior to addition of LY294002. Reactivation was quantified by scoring the percentage of wells expressing  GFP fluorescence at the indicated days. The percentage of wells expressing GFP-positive was scored as the mean ± SEM (n=3 biological replicates). Reactivation rates were quantified from 30 wells for each condition, 3 biological replicates."
NCBI gene IDs:
"Flag: 
Myc: 
Gadd45b: 299626"
Figure legend: "(F) Ectopic expression of Gadd45b-Myc-Flag  suppressed viral  ICP4 mRNA expression. Latently-infected SCG neurons were transduced with Gadd45b for 3 days and then treated with LY294002 and WAY-150138 for 72 hrs. Viral ICP4 mRNA was quantified by qRT-PCR. 3 biological replicates. The bars and error bars are mean ± SD. Data information: P values equal to or less than 0.05 were considered significant, asterisks denote statistical significance (*, p&lt;0.05; ***, p&lt;0.001; ****, p&lt;0.0001). P values are calculated using two-tailed unpaired Student's t test. P values &gt; 0.05 were not significant (ns)."
NCBI gene IDs:
"Flag: 
Myc: 
Gadd45b: 299626
ICP4: 2703392"
Figure legend: "(G)  Immunoblots for viral  ICP4  protein in latent SCG neurons treated with LY294002  and WAY-150138 in the presence or absence of  Gadd45b-Myc-Flag was quantified by ImageJ (n=3 biological replicates). Data information: P values equal to or less than 0.05 were considered significant, asterisks denote statistical significance (*, p&lt;0.05; ***, p&lt;0.001; ****, p&lt;0.0001). P values are calculated using two-tailed unpaired Student's t test. P values &gt; 0.05 were not significant (ns)."
NCBI gene IDs:
"Flag: 
Myc: 
Gadd45b: 299626"
Figure legend: "c, Knockdown of NetB  in the  eye disc  of GMR-RasV12 flies  enhances survival. d, NetB  heterozygous mutant flies survive  better over  Rasv12-induced oncogenic stress. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test (c) and two-tailed unpaired t-test d, *P &lt; 0.05, **P &lt;0.01, ****P &lt;0.0001."
NCBI gene IDs:
"NetB: 32400
Ras: 41140"
Figure legend: "f, qRT-PCR  with mRNA from the  eye disc confirms higher expression of NetB  in GMR-RasV12 flies  Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by two-tailed unpaired t-test *P &lt; 0.05, **P &lt;0.01, ****P &lt;0.0001."
NCBI gene IDs:
"NetB: 32400
Ras: 41140"
Figure legend: "g-h, Oncogenic Ras  expression in the eye disc  induces NetB  protein, which was detected by GFP  signal using CPTI-000748, a protein trap of NetB. Single confocal  z-section images are shown. i, Quantification of GFP  signals in g and h. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by two-tailed unpaired t-test *P &lt; 0.05, **P &lt;0.01, ****P &lt;0.0001. Scale bar, 50 μm."
NCBI gene IDs:
"Ras: 41140"
Figure legend: "a-b, The amount of NetB  protein increases in the fat body GMR-RasV12 (a) compared to control flies  (b).  CPTI-000748, a protein trap of NetB, labels endogenous NetB. Data information: Scale bar, 20 μm (a-b"
NCBI gene IDs:
"Ras: 41140"
Figure legend: "e-h, Ectopic expression of GFP-tagged NetB  in the  eye disc induces high levels GFP-NetB  protein in the fat body. Single confocal z-section are shown images (e and g). Data information: Scale bar, 20 μm f-h 50 μm"
NCBI gene IDs:
"GFP: 
NetB: 32400"
Figure legend: "j-k, Ectopic expression of GFP-tagged NetB  in the eye disc leads to detection of GFP  signals in the hemolymph. Data information: Scale bar, 20 μm j-k)"
NCBI gene IDs:
"GFP: 
NetB: 32400"
Figure legend: "a, Knockdown of InR  (InR) or expressing a dominant-negative form of InR  (InR-DN) in the  fat body using CG-Gal4  increases survival  over oncogenic Ras  expression in the imaginal disc. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test (a NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"Gal4: 855828
InR: 42549
Ras: 41140"
Figure legend: "c, qRT-PCR  demonstrates that TMLHE  expression in the fat body  is reduced by Ras  expression in the eye disc and reversed by insulin  inhibition in the fat body. d, qRT-PCR demonstrates that NetB  knockdown in the eye disc increases TMLHE  expression in the fat body. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparison test (c and d) NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"TMLHE: 42421
insulin: 42549
NetB: 32400
Ras: 41140"
Figure legend: "e, Knockdown of unc-5, but not other NetB  receptors (Fra  or Dscam1), in the  fat body increases TMLHE expression. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test e). NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"TMLHE: 42421
Dscam1: 35652
Fra: 36377
unc-5: 36703"
Figure legend: "b, Local expression of oncogenic Ras  in the eye disc  decreases the amount of carnitine  in the fat body. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by two-tailed unpaired t-test NS, not significant, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"Ras: 41140"
Figure legend: "c, TMLHE  knockdown in the  fat body  aggravates organismal survival  over the oncogenic stress. d, In the absence of GMR-RasV12, inhibition of TMLHE  in the fat body is not sufficient to induce organismal death. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by two-tailed unpaired t-test NS, not significant, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"TMLHE: 42421
Ras: 41140"
Figure legend: "g, Feeding of  acetyl-CoA precursor (acetate) makes GMR-RasV12 flies survive  even with TMLHE  knockdown in the fat body. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and the statistical significance was determined by two-tailed unpaired t-test NS, not significant, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"TMLHE: 42421
Ras: 41140"
Figure legend: "e, qRT-PCR with mRNA from the adult gut  confirms higher expression of NetB  in esg ts&gt;RasV12 flies  after oncogenic Ras  induction for 10 days. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and n represents the number of flies  that were analyzed. The statistical significance was determined by two-tailed unpaired t-test NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"esg: 34903
NetB: 32400
Ras: 41140"
Figure legend: "f-g, Oncogenic Ras  expression in the adult gut  induces NetB  protein, which was detected by GFP  signal using CPTI-000748, a protein trap of NetB. Ras  was induced for 10 days. Single confocal  z-section images are shown. h, Quantification of GFP  signals in f and g. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and n represents the number of flies  that were analyzed. The statistical significance was determined by two-tailed unpaired t-test NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001. Scale bar, 50 μm."
NCBI gene IDs:
"Ras: 41140"
Figure legend: "i, Knockdown of NetB  in the adult gut  of esg ts&gt;RasV12 flies  enhances survival. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and n represents the number of flies  that were analyzed. The experiments were repeated independently at least twice with similar results The statistical significance was determined by log-rank (Mantel-Cox) test NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"esg: 34903
NetB: 32400
Ras: 41140"
Figure legend: "l, Knockdown of TMLHE  in the fat body  of adult flies  shortened lifespan of esgts&gt;RasV12 flies  but not of control flies. Data information: Data points indicate biological replicates. Data are mean ± s.e.m. and n represents the number of flies  that were analyzed. The experiments were repeated independently at least twice with similar results The statistical significance was determined by log-rank (Mantel-Cox) test l). NS, not significant, *P &lt; 0.05, **P &lt;0.01, ***P &lt;0.001, ****P &lt;0.0001."
NCBI gene IDs:
"TMLHE: 42421
esg: 34903
Ras: 41140"
Figure legend: "A. Relative levels of OMA1 mRNA in colorectal cancer tissues (n=105) compared with adjacent normal tissues (n=43) from colorectal cancer patients were shown (using the GEO dataset GSE21510). The data are presented as mean ± SEM, and statistical significance was determined by a Mann-Whitney test. ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "B. Kaplan-Meier curves were constructed to analyze and compare between patients with high and low levels of OMA1 in colorectal cancer samples from the GEO dataset GSE17537. \"Low\" indicates patients with OMA1 mRNA levels less than the median. \"High\" indicates patients with OMA1 mRNA levels greater than the median. Statistical analysis was performed using log-rank tests, n = 55, p=0.0180."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "D. Control and OMA1 KO HCT116 cells were cultured in hypoxia (1% O2) for 0 (normoxia), 24, or 48 hours. Cell lysates were then assessed by Western blotting with antibodies against OPA1, OMA1, or β-Actin. β-Actin was used as a loading control. Representative immunoblots were from n = 3 independent experiments."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "E. OPA1-null mouse embryonic fibroblasts (MEFs) expressing control (empty vector) or OPA1 isoform 1 were cultured in normoxia or hypoxia (1% O2) for 24 hours. Cell lysates were analyzed by Western blotting with anti-OPA1 or anti-Tubulin antibody. Representative immunoblots were from n = 3 independent experiments."
NCBI gene IDs:
"OPA1: 74143"
Figure legend: "F. WT, OMA1 KO and OMA1 KO HCT116 cells expressing WT-OMA1(OMA1-Flag) or proteolytic inactive OMA1(E324Q-Flag) were cultured in hypoxia (1% O2) for 0, 24, or 48 hours, and the cell lysates were assessed by western blot with antibodies against OMA1, and GAPDH. β-Actin was used as a loading control (representative data from three independent experiments). The asterisk indicates a nonspecific band. G. The relative protein levels were evaluated by densitometry analysis using ImageJ software and were quantified for the ratio of OMA1-Flag/β-Actin or E324Q-Flag/β-Actin in hypoxia for 0, 24 or 48 hours (n = 3 independent experiments). The data are presented as mean ± SD. "
NCBI gene IDs:
"Flag: 
OMA1: 115209"
Figure legend: "B. Body weight changes of WT (n=11) and OMA1-/- (n=12) mice during AOM/DSS treatment. Data are presented as mean ± SEM. Statistical significance was assessed by a two-way ANOVA, **p &lt; 0.01."
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "C. Typical intestine images on day 105 after mice were euthanized. D. Colorectal lengths (C) of WT (n=11 biological replicates) and OMA1-/- (n=12 biological replicates) mice were measured. Data with error bars are presented as mean ± SEM. Statistical significance was assessed by unpaired student's t-test, *p &lt; 0.05. "
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "E. Representative images of colorectal tumors from WT (left) and OMA1-/- (right) mice. F. The number of tumors in WT (n=11) and OMA1-/- (n=12) mice was measured. Each dot represents the tumor number of one individual mouse. Data with error bars are presented as mean ± SEM. Statistical significance was assessed by unpaired student's t-test, ***p &lt; 0.001. "
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "G, H. The number of tumors of different sizes (diameter) in each mouse (G) and the distribution of different tumor sizes (H) were shown (WT n=11 biological replicates, and OMA1-/- n=12 biological replicates). Results are shown as mean ± SEM. p-values were calculated by unpaired student's t-test, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "I. Histological analysis of colorectal tumors of WT and OMA1-/- mice was shown by hematoxylin and eosin (H&amp;E) staining."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "J. Relative mRNA expression levels of pro-inflammatory genes (TNFα, IL-6, COX-2, CCL2) in colorectal homogenates of WT and OMA1_KO mice treated with AOM/DSS (n =4-5 per genotype). The data represent the mean ± SEM., n = 3 independent experiments, and statistical significance was determined by a two-tailed student's t-test. *p &lt; 0.05, **p &lt; 0.01."
NCBI gene IDs:
"CCL2: 20296
COX-2: 17709
IL-6: 16193
OMA1: 67013
TNFα: 21926"
Figure legend: "K. The colorectal tissues of WT and OMA1-/- mice were stained with Ki67."
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "A, B. Control or OMA1 KO HCT116 cells expressing control (empty vector) or OMA1-Flag were maintained in normoxia or hypoxia (1% O2) for 24 hours. The glucose uptake (A) and lactate production (B) were measured by using microplate reader, and the values were normalized to the protein concentration. Error bars indicate the mean ± SD of three independent experiments, statistical significance was assessed by a two-way ANOVA, *P &lt; 0.05. Cells were lysed and assessed by western blot with antibodies against OMA1 and GAPDH (A). The asterisk indicates a nonspecific band."
NCBI gene IDs:
"Flag: 
OMA1: 115209"
Figure legend: "C. Control and OMA1 KO HCT116 cells were treated with DMSO or 1mM DMOG in fresh medium for 12 h. Subsequent injections of glucose, oligomycin (Oligo), and 2-deoxy-glucose (2-DG) were performed as indicated. The extracellular acidification rates (ECAR) were measured with a Seahorse Extracellular Flux Analyzer XF96. The data are presented as mean ± SD (n = 3 independent experiments)."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "E, F. Lactate production (E) and ATP production (F) were measured in control or OPA1 KO HCT116 cells upon DMSO or 2-DG treatment. Error bars are presented as mean ± SD by a two-way ANOVA (n = 3 independent experiments). N.S., not significant, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"OPA1: 74143"
Figure legend: "G, H. Control or OPA1 KO HCT116 cells were maintained in normoxia or hypoxia (1% O2) for 24 hours. The glucose uptake (G) and lactate production (H) were measured, and the values were normalized to the protein concentration. Statistical significance was assessed by a two-way ANOVA; error bars are presented as mean ± SD of three independent experiments; N.S., not significant, *p &lt; 0.05, **p &lt; 0.01."
NCBI gene IDs:
"OPA1: 74143"
Figure legend: "A. Control and OMA1 KO HCT116 cells were cultured in normoxia or in hypoxia (1% O2) for 24 or 48 hours. The whole cell lysates were analyzed by Western blotting with antibodies against HIF-1α, HK2, LDHA, PKM2, GPI, OMA1, or β-Actin. The relative protein levels were further evaluated by densitometry analysis using ImageJ software and were presented as bar graphs in the right panels. Error bars are presented as mean ± SD by a two-way ANOVA (n = 3 independent experiments). *p&lt;0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "B. Control or OMA1 KO Hela cells were untreated (-) or CoCl2-treated (+) for 24 hours with 10 mM MG132 or vehicle added for the last 4 hours. Cell lysates were assessed by Western blotting with antibodies against HIF-1α or β-Actin. Relative protein levels were further evaluated by densitometry analysis. Error bars indicate the mean ± SD by a two-way ANOVA (n = 3 independent experiments). *p&lt;0.05, **p &lt; 0.01."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "C. Dihydroethidium (DHE) staining of optimum cutting temperature (OCT) covered tissues from the colons of WT and OMA1-/- mice; DAPI was used as counterstain. DHE was shown in red color, while DAPI in blue color (representative data from three independent experiments). D. Quantification of DHE staining. Error bars are presented as mean ± SD (n=3 biological replicates), statistical significance was assessed by a two-way ANOVA, **p &lt; 0.01, ***P&lt; 0.001. "
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "E. Control or OMA1 KO HCT116 cells cultured in normoxia or hypoxia (1% O2) for 12 hours were stained with mitoSOX, and then analyzed by confocal microscopy (representative data from three independent experiments). F. The fluorescence intensity of mitoSOX was further analyzed by ImageJ software. Bars indicate the mean ± SD of three independent experiments by a two-way ANOVA, ***p &lt; 0.001. "
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "G-I. Control or OMA1_KO HCT116 cells were cultured in normoxia or hypoxia for 12 hours, and were treated with DMSO, NAC (10 mM) or Rotenone (1 μM) for 12 hours at the same time. The whole cell lysates were analyzed by Western blotting with antibodies against HIF-1α, and Tubulin (G). The glucose uptake (H) and lactate production (I) were measured using microplate reader, and the values were normalized to the protein concentration. Error bars indicate the mean ± SD (n = 3 independent experiments), statistical significance was assessed by two-way ANOVA. N.S., not significant, *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "A. 293T cells were transiently co-transfected with empty vector and NDUFB5-MYC, or OMA1-E324Q-Flag and NDUFB5-MYC. Cell lysates were used for co-immunoprecipitation with anti-Flag M2 affinity gel at 4°C overnight, followed by Western blotting with anti-Flag or anti-MYC antibodies (n = 3 independent experiments)."
NCBI gene IDs:
"Flag: 
MYC: 
NDUFB5: 4711
OMA1: 115209"
Figure legend: "B. 293T cells were transiently co-transfected with empty vector and NDUFB6-GFP, or OMA1-E324Q-Flag and NDUFB6-GFP. Cell lysates were used for co-immunoprecipitation with anti-Flag M2 affinity gel at 4°C overnight, followed by Western blotting with antibodies against Flag or GFP (n = 3 independent experiments)."
NCBI gene IDs:
"Flag: 
GFP: 
GFP.: 
NDUFB6: 4712
OMA1: 115209"
Figure legend: "C. 293T cells were transiently co-transfected with empty vector and COX4L1-GFP, or OMA1-E324Q-Flag and COX4L1-GFP. Cell lysates were used for coimmunoprecipitation with anti-Flag M2 affinity gel at 4°C overnight, followed by Western blotting with anti-Flag or anti-GFP antibodies (n = 3 independent experiments)."
NCBI gene IDs:
"Flag: 
GFP: 
GFP.: 
COX4L1: 1327
OMA1: 115209"
Figure legend: "D. 293T cells were transiently co-transfected with empty vector and NDUFA4-GFP, or OMA1-E324Q-Flag and NDUFA4-GFP. Cell lysates were used for coimmunoprecipitation with anti-Flag M2 affinity gel at 4°C overnight, followed by Western blotting with antibodies against Flag or GFP (n = 3 independent experiments)."
NCBI gene IDs:
"Flag: 
GFP: 
GFP.: 
NDUFA4: 4697
OMA1: 115209"
Figure legend: "E. HCT116 cells stably expressing control (empty vector) or OMA1-Flag, and HCT116 cells infected with control or shOMA1 (OMA1 knockdown) lentiviral particles and further cultured for 5 days, were then treated with DMSO or 20 μm CCCP for 4 hours. Cell lysates were analyzed by Western blotting with antibodies against NDUFA4, COX4L1, NDUFB5, DNUFB6, MT-CO2, OMA1 or Tubulin. The black arrowhead indicated the full-length COX4L1 or NDUFB6, and the red arrowhead pointed to the cleaved band. The asterisk indicates a nonspecific band."
NCBI gene IDs:
"Flag: 
OMA1: 115209"
Figure legend: "F. WT, OMA1 KO and OMA1 KO HCT116 cells expressing WT-OMA1(OMA1-Flag) or proteolytic inactive OMA1(E324Q-Flag) were treated with CHX (100 µg/ml) plus CCCP (20 µM) for 0, 2, 4, 6 hours. The cell lysates were then assessed by western blot with antibodies against NDUFA4, COX4L1, NDUFB5, NDUFB6, OMA1 or HSPD1 (representative data from three independent experiments). The asterisk indicates a nonspecific band."
NCBI gene IDs:
"Flag: 
OMA1: 115209"