EMBO/SourceData huggingface dataset:
Figure legend: "A. Representative images of  Sir3-mCherry and Sae2-GFP signal in WT and SIR3 overexpressing cells. Scale bars are 2 μm."
NCBI gene IDs:
"SIR3: 851163"
Figure legend: "B.   Sir3-binding at TEL6R in untagged, WT, sir3Δ cells or in cells overexpressing Sir3 (oeSir3). Binding is probed by  ChIP-qPCR 0.2 (red arrows) and 1kb (blue arrows) from  telomeres and at the OGG1  control locus using antibodies against Sae2-GFP. The mean of three independent biological replicates is shown and error bars correspond to the variation between replicates (SEM)."
NCBI gene IDs:
"OGG1: 854942
sir3: 851163
Sir3: 851163"
Figure legend: "C. Co-immunoprecipitation between  Sir3 and Sae2-GFP from cells overexpressing  Sir3, analysed by Western blot with anti-GFP and anti-Sir3 antibodies. D. Co-immunoprecipitation between Sir3  and Sae2-GFP from WT cells using antibodies against Sae2-GFP, analysed by Western blot."
NCBI gene IDs:
"Sir3: 851163"
Figure legend: "D.  Yeast two-hybrid interaction  analysis  between Sae2C and Sir3SaID domains in WT or sir4Δ cells. Growth on -His + 3AT  and blue coloration on X-gal indicate an  interaction."
NCBI gene IDs:
"sir4: 851813"
Figure legend: "B. Representative images of two hybrid assays in the yKD1991 strain testing the interaction of the WT or the mutant SIR3SaID fragment isolated from the screen with SAE2C or SIR4C. C. Representative images of two hybrid assays  testing the interaction of the WT or the mutant SIR3SaIDT557I fragment with SAE2C or SIR4C."
NCBI gene IDs:
"SIR3: 851163"
Figure legend: "A. Survival frequencies after    DSB induction at  LYS2 locus in WT or sae2Δ strains where the Sir3SaID or Sir3SaIDT557I domains are overexpressed from a GPD promoter at the SIR3 locus. Error bars indicate  survival  standard error (SEM) of at least three independent experiments. Data information: Significance was determined using 2-tailed, unpaired Student's t test. *P-value 0.01 to 0.05, significant; **P-value 0.001 to 0.01, very significant; ****P &lt; 0.0001, extremely significant; P ≥ 0.05, not significant (ns)."
NCBI gene IDs:
"LYS2: 852412
sae2: 852700
Sir3: 851163
SIR3: 851163
GPD: 853106"
Figure legend: "B. Representative images of Sae2-GFP  in WT cells and in cells overexpressing either full-length  Sir3 or the sir3SaID domain. Scale bars are 2 μm."
NCBI gene IDs:
"Sir3: 851163
sir3: 851163"
Figure legend: "G. Co-immunoprecipitation between Sae2-GFP and  Sir3 from untagged, Sae2-GFP WT cells, and Sae2-GFP cells overexpressing WT Sir3  (oeSir3, WT), Sae2-GFP sir3Δ or Sae2-GFP overexpressing the  sir3-T557I mutant (oeSir3, T557I) using antibodies against  Sae2-GFP, analysed by Western blot with anti-GFP and anti-Sir3 antibodies."
NCBI gene IDs:
"GFP: 
Sae2: 852700
Sir3: 851163
sir3: 851163"
Figure legend: "A. Survival  frequencies after  DSB induction at LYS2 locus in the indicated strains. Error bars indicate survival  standard error (SEM) of at least three independent experiments. Data information: Significance was determined using 2-tailed, unpaired Student's t test. *P-value 0.01 to 0.05, significant; **P-value 0.001 to 0.01, very significant; ***P-value 0.0001 to 0.001, extremely significant; ****P &lt; 0.0001, extremely significant; P ≥ 0.05, not significant (ns)."
NCBI gene IDs:
"LYS2: 852412"
Figure legend: "C.  Survival frequencies after  DSB induction at LYS2 locus in the indicated strains. Insertion of the strong TEF1p promoter upstream of the SIR4 ORF leads to  Sir4 overexpression. Insertion of the ADH1p promoter upstream of SIR3  leads to mild Sir3 overexpression. Error bars indicate survival  standard error (SEM) of at least three independent experiments. Data information: Significance was determined using 2-tailed, unpaired Student's t test. *P-value 0.01 to 0.05, significant; **P-value 0.001 to 0.01, very significant; ***P-value 0.0001 to 0.001, extremely significant; ****P &lt; 0.0001, extremely significant; P ≥ 0.05, not significant (ns)."
NCBI gene IDs:
"ADH1: 854068
LYS2: 852412
SIR3: 851163
Sir3: 851163
SIR4: 851813
Sir4: 851813
TEF1: 856195"
Figure legend: "E. Representative images of two hybrid assays testing the interaction  between the full-length Sir3 and full-length Sae2 proteins in WT cells expressing or not high levels of Sir4  (oeSir4 and WT respectively)."
NCBI gene IDs:
"Sir4: 851813"
Figure legend: "A. Relative fold enrichment of Sae2 at 0.2 kb from I-SceI site was evaluated by qPCR after ChIP with anti-GFP antibodies. The error bars indicate the variation between at least three biological replicas (SEM). B. Relative fold enrichment of  Sae2 at 0.2 kb from TEL6R after  DSB  induction at the LYS2 I-SceI site was evaluated by qPCR after ChIP with anti-GFP  antibodies. The error bars indicate the variation between at least three biological replicas (SEM). Data information: Significance was determined using 2-tailed, unpaired Student's t test. *P-value 0.01 to 0.05, significant; **P-value 0.001 to 0.01, very significant; ***P-value 0.0001 to 0.001, extremely significant; ****P &lt; 0.0001, extremely significant; P ≥ 0.05, not significant (ns)."
NCBI gene IDs:
"LYS2: 852412
I-SceI: 854590"
Figure legend: "D. Survival frequencies after  DSB induction at LYS2  locus in  sae2-E267E mutant cells overexpressing or not full-length Sir3  (oeSir3). Error bars indicate survival  standard error (SEM) of at least three independent experiments. Data information: Significance was determined using 2-tailed, unpaired Student's t test. *P-value 0.01 to 0.05, significant; **P-value 0.001 to 0.01, very significant; ***P-value 0.0001 to 0.001, extremely significant; ****P &lt; 0.0001, extremely significant; P ≥ 0.05, not significant (ns)."
NCBI gene IDs:
"LYS2: 852412
sae2: 852700
Sir3: 851163"
Figure legend: "A TRAF6 expression in differentiating CD4+ T cells. Naïve CD4+ T cells were obtained from wild type C57BL/6 mice by FACS and activated with anti-CD3/CD28 (1µg, and 2µg/ml) for the indicated times in the presence of distinct Thelper lineage-directing cytokines or under neutral activation conditions (Th0). After total RNA extraction and cDNA conversion, RT-PCR determined of Traf6 mRNA in differentiating Th0, Th1, Th17 and iTregs was determined."
NCBI gene IDs:
"Traf6: 22034
TRAF6: 22034"
Figure legend: "B TRAF6 mRNA expression by human Tregs and non-Treg CD4+ T cell. Human Tregs (CD3+/CD4+/CD8-/CD25HIGH/CD127low/CD39+) and non-Treg CD4+ T cells (CD3+/CD4+/CD8-/CD25-) were obtained from the peripheral blood of healthy donors by FACS after Ficoll-Paque PLUS gradient centrifugation and magnetic bead enrichment of CD4+ T cells. TRAF6 mRNA was measured by qRT-PCR."
NCBI gene IDs:
"TRAF6: 22034"
Figure legend: "C, D Evidence of lymphoproliferative disease in Traf6fl/flFoxp3Cre+ mice. (C) Spleens and lymph nodes were recovered from Traf6fl/flFoxp3Cre+ mice and Traf6fl/fl littermates at 8 weeks of age (Scale bars: 5 mm). (D) The cellularity of the lymphoid tissues of Traf6fl/flFoxp3Cre+ mice and their Traf6fl/fl Foxp3Cre- littermates was determined (8 mice/group)."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Traf6: 22034"
Figure legend: "E, H Effect of Treg-specific TRAF6-deficiency on baseline T cell activation. The frequencies of effector cells (CD44high/CD62Llow), memory cells (CD44high/CD62Lhigh) and naïve cells (CD44low/CD62Lhigh) in the CD4+ T cell compartments of Traf6fl/fl and Traf6fl/flFoxp3Cre+ mice were determined by flow cytometry (5 mice/group)."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
TRAF6: 22034
Traf6: 22034"
Figure legend: "F, I Effect of Treg-specific TRAF6-deficiency on baseline T cell activation. The frequencies of effector cells (CD44high/CD62Llow), memory cells (CD44high/CD62Lhigh) and naïve cells (CD44low/CD62Lhigh) in the CD8+ T cell compartments of Traf6fl/fl Foxp3Cre- (wild type) and Traf6fl/flFoxp3Cre+ mice were determined by flow cytometry (5 mice/group)."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
TRAF6: 22034
Traf6: 22034"
Figure legend: "G, J Impact of TRAF6-expression on in vitro Treg differentiation. naïve CD4+ T cells were isolated from Traf6fl/flFoxp3Cre+ and Traf6fl/fl mice and differentiated into iTregs. Conditions of sub-optimal TGFβ concentrations (0.5ng/ml, 0.05ng/ml) were tested as well and intracellular FOXP3 was measured after 4 days."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
TRAF6: 22034
Traf6: 22034"
Figure legend: "A In vivo suppressive function of WT and Traf6fl/flFoxp3Cre+ Tregs. Tregs from the indicated mice (CD45.2+) were isolated by FACS, as were naïve (CD62Lhigh/CD25-) CD4+ responder T cells from congenically distinct (CD45.1+) donor mice. Tregs and Tresponders were mixed at a 1:5 (2x105:10x105) ratio before injection into Rag2-/- mice. 7 days later, spleens were harvested, and the relative frequencies and absolute numbers of each transferred cell populations were determined by flow cytometry."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Rag2: 19374
Traf6: 22034"
Figure legend: "B Implanted B16 melanoma growth in Traf6fl/flFoxp3Cre+ and wild type (Traf6fl/flFoxp3Cre-) mice. 1x105 B16F10 cells were injected subcutaneously (s.c.) into the shaved flanks of the indicated mice (n= 5/group). Tumor volumes were monitored every 3 days."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Traf6: 22034"
Figure legend: "C, D Proinflammatory cytokine production in tumor-bearing mice with and without Treg-specific TRAF6 expression. Cell suspensions of the tumor-draining lymph node and tumor-infiltrating leukocytes (TILs) were recovered after 21 days of tumor growth. Ex vivo stimulation with PMA and ionomycin in the presence of Golgistop for 5 hour preceded intracellular staining for IFNγ and IL-17 and flow cytometry analysis."
NCBI gene IDs:
"TRAF6: 22034"
Figure legend: "E, F FOXP3 expression by CD4+ T cells in tumor bearing Traf6fl/flFoxp3Cre+ and wild type (Traf6fl/flFoxp3Cre-) mice. The frequency of FOXP3+ cells in the CD4+ cells of tumor-draining lymph nodes (dLN), peripheral, non-tumor draining lymph nodes (pLN), spleens, and TILs were determined by flow cytometry."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Traf6: 22034"
Figure legend: "A Assessment of K63-type ubiquitination of FOXP3 upon expression of wild type and catalytically deficient TRAF6 mutants. 293T cells were transfected with the given combinations of vectors encoding wild type TRAF6, the enzymatically deficient L74H or C70A TRAF6 mutants, HA-FOXP3, and FLAG-labeled ubiquitin molecules possessing a single lysine residue (K63) that restrain the possible ubiquitin monomer linkages on polyubiquitinated proteins to the K63-type only. Cells were lysed and proteins modified by K63-ubiquitin chains were recovered from lysates by pulling down using a bead-immobilized K63-specific TUBE reagent. The presence of FOXP3 among these modified proteins was determined by immunoblotting as were levels of FOXP3 and TRAF6 in the pre-IP whole cell lysate (WCL)."
NCBI gene IDs:
"FLAG: 
HA: 
ubiquitin: 
FOXP3: 20371
TRAF6: 22034"
Figure legend: "B Co-immunoprecipitation (co-IP) of FOXP3 with TRAF6 and its facilitation of K63-linked ubiquitination. 293T cells expressing the indicated constructs encoding HA-FOXP3 and FLAG-TRAF6 were lysed and incubated with bead immobilized anti-FLAG antibodies. FOXP3 molecules co-IPed in this manner were resolved by SDS PAGE and detected by immunoblotting with anti-HA antibodies."
NCBI gene IDs:
"FLAG: 
HA: 
FOXP3: 20371
TRAF6: 22034"
Figure legend: "C　293T cells were transfected with the indicated combinations of expression constructs encoding HA-FOXP3, Myc-TRAF6, and FLAG-tagged ubiquitin molecules - either wild type (FLAG-Ub), or a variant with a K-to-R mutation at either lysine residue 48 (K48R) or 63 (K63R) responsible for preventing K48- and K63-type polyubiquitination, respectively. ."
NCBI gene IDs:
"FLAG: 
HA: 
Myc: 
ubiquitin: 
FOXP3: 20371
TRAF6: 22034"
Figure legend: "E Degree of K63 ubiquitination in the cellular FOXP3 pools of Traf6fl/flFoxp3Cre+ and Traf6wt/wtFoxp3Cre+mice. CD4+/YFP+ cells from Traf6fl/flFoxp3Cre+ (&quot;Traf6-/-&quot;) or wild type Traf6wt/wtFoxp3Cre+mice (&quot;Traf6+/+&quot;) were isolated by FACS, lysed and either total FOXP3 (right) or K63-ubiquitin-modified proteins (left) were immunoprecipitated. Levels of polyubiquitinated FOXP3 were observed in each by probing with antibodies specific for anti-K63-ubiquitin and FOXP3, respectively, and levels of total FOXP3 and actin were also measured by immunoblot analysis of whole cell lysate."
NCBI gene IDs:
"Cre: 2777477
FOXP3: 20371
Foxp3: 20371
Traf6: 22034"
Figure legend: "A Immunoblot analysis of TRAF6-mediated ubiquitination of wild type, and mutant FOXP3 molecules. 293T cell lines were transfected with a normal HA-tagged Foxp3 construct, another encoding a ubiquitination resistant mutant in which all lysine residues were replaced by arginines (20R), or one of 20 single lysine-containing constructs with only the indicated lysine residue available for modification. These cell lines also received expression vectors encoding TRAF6 and ubiquitin molecules labeled with Myc and FLAG tags, respectively. Negative controls did not receive TRAF6 or expressed FOXP3 alone. Labeled FOXP3 proteins were pulled down from cell lysates (anti-HA) and ubiquitinated species were visualized by immunoblotting for FLAG."
NCBI gene IDs:
"FLAG: 
HA: 
Myc: 
ubiquitin: 
Foxp3: 20371
TRAF6: 22034"
Figure legend: "B Assessing the impact of K262 mutation on FOXP3 ubiquitination by TRAF6. 293T cells were transfected with combinations of expression constructs encoding Myc-TRAF6, FLAG-ubiquitin, and an HA-tagged FOXP3 molecule that was either normal (HA-FOXP3) or possessed a K-to-R mutation at residue 262 (K262R). FOXP3 proteins were pulled down with anti-HA beads and either ubiquitinated proteins or total FOXP3 proteins were detected by probing for FLAG and HA, respectively."
NCBI gene IDs:
"FLAG: 
HA: 
Myc: 
ubiquitin: 
FOXP3: 20371
TRAF6: 22034"
Figure legend: "A Impact of K63-ubiquitination loss on FOXP3's gene silencing capacity. Jurkat T cells transfected with a wild type Foxp3 expression vector, one encoding the K262R mutant, or an empty (control) vector also received a duel luciferase reporter construct where luciferase expression was under the control of the Il2 promoter. After activation with PMA and ionomycin for 8 hours, luciferase activity was assayed."
NCBI gene IDs:
"luciferase: 
Foxp3: 20371
FOXP3: 20371
Il2: 3558"
Figure legend: "B IL-2 production in the presence of wild type and K63-ubiquitination resistant FOXP3. Naïve CD4+ T cells isolated from Traf6fl/flCD4Cre+ or WT mice were activated overnight by anti-CD3/CD28 antibodies, then transduced by retroviruses carrying normal Foxp3, the K262R mutant, or an empty vector. Ires-GFP in the retroviral vector serves as an internal control. GFP+ cells were sorted out 48 hours post-transduction. The sorted cells were cultured for one additional day. Culture supernatants were collected for measuring the levels of IL-2 by ELISA."
NCBI gene IDs:
"CD4: 12504
Cre: 2777477
Foxp3: 20371
FOXP3: 20371
Traf6: 22034"
Figure legend: "C Cellular distribution of wild type and K262R FOXP3. Hela cells were transfected with expression vectors encoding either wild type FOXP3 or the K262R mutant. The relative overlap of FOXP3 protein signal and DAPI-stained nuclei was observed by fluorescence microscopy. (blue: DAPI, green: FOXP3, Scale bars: 50 μm)."
NCBI gene IDs:
"FOXP3: 20371"
Figure legend: "D Immunostaining of FOXP3 in Traf6fl/flCD4Cre+ and wild type (Traf6fl/flCD4Cre-) derived suspensions of lymph node and spleen cells. (blue: DAPI, green: FOXP3, Scale bars: 50 μm)."
NCBI gene IDs:
"CD4: 12504
Cre: 2777477
Traf6: 22034"
Figure legend: "E Quantification of FOXP3 distribution in murine Tregs. The degree of FOXP3-nuclear coIocalization in CD4+/FOXP3+ cells isolated from the lymph nodes and spleens of Traf6fl/flCD4Cre+ and wild type (Traf6fl/flCD4Cre-) mice was determined by Imagestream analysis, and the frequencies of cells with either a low or high probability of nuclear FOXP3 distribution are shown (red: nuclear stain, green:FOXP3, Scale bars: 10 um)."
NCBI gene IDs:
"CD4: 12504
Cre: 2777477
Traf6: 22034"
Figure legend: "The in vitro suppressive potency of wild type- and K262R Foxp3-expressing T cells. Naïve CD4+ T cells were purified from Thy1.1+ BALB/c mice and subjected to retroviral transduction to express either wild type FOXP3 or a K262R mutant resistant to ubiquitination that lysine residue 262. These engineered &quot;Tregs&quot; were then co-cultured with naïve responder CD4+ T cells stained with CFSE at the indicated ratios and activated with anti-CD3/CD28 antibodies. Dilution of CFSE signal by the responder populations (i.e. proliferation) was assessed by flow cytometry."
NCBI gene IDs:
"Foxp3: 20371
FOXP3: 20371"
Figure legend: "The in vitro suppressive potency of wild type- and K262R Foxp3-expressing T cells. Naïve CD4+ T cells were purified from Thy1.1+ BALB/c mice and subjected to retroviral transduction to express either wild type FOXP3 or a K262R mutant resistant to ubiquitination that lysine residue 262. These engineered \"Tregs\" were then co-cultured with naïve responder CD4+ T cells stained with CFSE at the indicated ratios and activated with anti-CD3/CD28 antibodies. Dilution of CFSE signal by the responder populations (i.e. proliferation) was assessed by flow cytometry."
NCBI gene IDs:
"Foxp3: 20371
FOXP3: 20371"
Figure legend: "C In vivo suppression of colitis by wild type- and K262R mutant-Foxp3 expressing T cells. 2x105 naïve CD4+ transductants from A were mixed with 1x106 colitogenic naïve CD4+ T cells from wild type mice and transferred i.v. into Rag2-/- mice. Rag2-/- mice receiving no cell transfers, naïve CD4+ T cells alone, or purified wild type Tregs served as controls (n= 6/group). Recipient mouse body weights were monitored weekly for each group."
NCBI gene IDs:
"Foxp3: 20371
Rag2: 19374"
Figure legend: "PKA activity after overexpression of SPATC1L or SPATC1LΔC. Graphs show the average PKA activity of Spatc1l-transfected cells (white bar) and Spatc1lΔC-transfected cells (black bar) in the presence or absence of cAMP, expressed relative to control cells (mock) as a percentage. Representative signals observed in the assay are shown above. The overexpression of SPATC1L was confirmed in every experiment by immunoblotting (data not shown)."
NCBI gene IDs:
"Spatc1l: 76573
SPATC1L: 76573"
Figure legend: "The amount of Cα associated with RIα in the presence or absence of SPATC1LΔC. After IP of RIα in cells overexpressing SPATC1LΔC or control, immunoblot analysis was performed using anti-Cα antibody. Graph shows densitometric analysis of Cα in relation to immunoprecipitated RIα in immunoblot analysis . In, input; IgG, immunoglobulin G from normal rabbit serum."
NCBI gene IDs:
"SPATC1L: 76573"
Figure legend: "PCR genotyping analysis of WT (Spatc1l+/+), heterozygous (Spatc1l+/-) and homozygous (Spatc1l-/-) mice."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "SPATC1L expression levels in testes obtained from WT, Spatc1l+/-, and Spatc1l-/- mice. An anti-GAPDH antibody was used as a control. Graph shows densitometric analysis of SPATC1L in relation to GAPDH in immunoblot analysis."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Percentage of females that became pregnant after mating with WT (white bar), Spatc1l+/- (gray bar), and Spatc1l-/- (black bar) male mice. Male mice used: WT, n = 5; Spatc1l+/-, n = 6; Spatc1l-/-, n = 7. WT female mice used for mating with the indicated genotype: WT, n = 30; Spatc1l+/-, n = 36; Spatc1l-/-, n = 42. Vaginal plugs were observed in all mated females."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Average litter size of pups produced by females mated with WT (white bar), Spatc1l+/- (gray bar), and Spatc1l-/- (black bar) male mice."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Macroscopic appearance of adult testes from an 8-week-old WT (Spatc1l+/+), heterozygous (Spatc1l+/-) and homozygous (Spatc1l-/-) mice. Scale bar = 2 mm."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Comparison of testis weight in WT (white bar), Spatc1l+/- (gray bar), and Spatc1l-/- (black bar) mice. The organs were trimmed of fat and weighed. The average testis weight/body weight ratios for WT, Spatc1l+/-, and Spatc1l-/- males were 0.0034±0.0001, 0.0038±0.0003, and 0.0031±0.0002, respectively. Data are presented as means ± s.d. (n = 7)."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Macroscopic appearance of the adult epididymis from WT, Spatc1l+/-, and Spatc1l-/- mice. Scale bar = 2 mm."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Epididymis weight in WT (white bar), Spatc1l+/- (gray bar), and Spatc1l-/- (black bar) mice. The average epididymis weight/body weight ratios for WT, Spatc1l+/-, and Spatc1l-/- males were 0.001±0.0001, 0.001±0.0001, and 0.001±0.00008, respectively. Data are presented as means ± s.d. (n = 7)."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Number of mature epididymal sperm from WT (white bar), Spatc1l+/- (gray bar), and Spatc1l-/- (black bar) mice. Sperm were collected from the vas deferens. The average number of mature sperm in WT, Spatc1l+/-, and Spatc1l-/- males was (4.06 ± 0.40) × 106, (3.7 ± 0.7) × 106, and (3.93 ± 0.90) × 106, respectively. Data are presented as means ± s.d. (n = 5)."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Percentage of headless sperm from cauda epididymides of WT (black), Spatc1l+/- (gray), and Spatc1l-/- (white) mice. The average percentage of headless sperm in WT, Spatc1l+/-, and Spatc1l-/- males was 6.3, 52.9 and 100, respectively. Each dot represents percentage of headless sperm in an individual mouse, and medians are represented as horizontal lines. (n=13, *P &lt; 0.001, Student's t-test)."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Morphological analyses of Spatc1l-KO epididymal sperm by SEM. Sperm were obtained from the vas deferens of 8-week-old WT (Spatc1l+/+) and KO (Spatc1l-/-) mice. Sperm neck regions are magnified in lower panels. AC, acrosomal cap; MP, mid piece; BP, basal plates. Scale bar = 2 μm (upper) and 0.5 μm (lower)."
NCBI gene IDs:
"Spatc1l: 76573"
Figure legend: "Immunoblot analyses of five candidate proteins in testis (E) and sperm (F) of WT and KO mice. Anti-GAPDH, anti-ADAM2, and anti-α-tubulin antibodies were used as controls. Loss of SPATC1L in KO testis was confirmed by immunoblotting with anti-SPATC1L antibody."
NCBI gene IDs:
"SPATC1L: 76573"
Figure legend: "The amount of CAPZB in mock and Spatc1lΔC-transfected cells. Anti-GAPDH antibody was used as control. Overexpression of SPATC1L in TM4 cells was confirmed by immunoblotting with anti-SPATC1L antibody. Graph shows densitometric analysis of CAPZB in pcDNA3.1 vector-transfected control cells (mock; white bar) and SPATC1LΔC-transfected cells (black bar), expressed relative to control cells (mock) as a percentage. The density of CAPZB in immunoblot analysis was calculated in relation to density of GAPDH."
NCBI gene IDs:
"Spatc1l: 76573
SPATC1L: 76573"
Figure legend: "Phosphorylation of CPAZB3 in the presence or absence of SPATC1L. Phosphorylation of purified CAPZB3 was labeled with 32P via PKA in the presence or absence of SPATC1L, and estimated by autoradiography. The amount of CAPZB3 protein was quantified by Coomassie Brilliant Blue (CBB) staining (lower). Graph shows densitometric analysis of phopho-CAPZB3 (p-CAPZB3) in reactions without (control; white bar) or with SPATC1L (black bar), expressed relative to control as a percentage."
NCBI gene IDs:
"SPATC1L: 76573"
Figure legend: "Observation of filamentous actin (F-actin) in the presence (lower) or absence (upper) of SPATC1L. F-actin in pEGFP vector-transfected control cells (mock; upper, green) and cells overexpressing SPATC1LΔC (lower, green) was visualized by staining using Phalloidin (red). White bar shows the length of transfected cell. Scale bar = 50 μm."
NCBI gene IDs:
"EGFP: 
SPATC1L: 76573"
Figure legend: "The length of cell overexpressing SPATC1LΔC compared with control. Graph shows the average of measured cell length in pEGFP vector-transfected control cells (mock; white bar) and Spatc1lΔC-transfected cells (black bar), expressed relative to control cells (mock) as a percentage."
NCBI gene IDs:
"EGFP: 
SPATC1L: 76573
Spatc1l: 76573"
Figure legend: "Cell surface H2-Kb levels of wild type and TAP1-/- BMDC transduced with control vector or a vector expressing hβ2m were analyzed."
NCBI gene IDs:
"β2m: 567
TAP1: 21354"