EMBO/SourceData huggingface dataset:
Figure legend: "  Fig. 7: Loss of VE-cadherin from lymphatic endothelial cells results in increased circumferential actin and a reduced number of focal adhesions. A, B Primary dermal LECs were isolated as described in appendix Fig. S8 A. PdLECs were used for up to four passages and control and VE-cadherin-deleted pdLECs had been cultured for the same number of passages. Cells were seeded at a density of 2.5 x 104 cells on gelatine-coated 15mm glass coverslips and co-stained after 3 days of culture for the actin cytoskeleton (Phalloidin) and either Vinculin (A) or FAK (B) for the visualization of focal adhesions. PROX1 staining in (A) identifies LECs, nuclei in (B) were counterstained with Hoechst. Scale bars correspond to 50 µm. (C, D) Bar diagrams show the number of focal adhesions/cell, which were enumerated using Image J from the Vinculin and FAK stainings shown in (A and B). Data are mean values + S.E.M. derived from three cell lines and 9 viewfields of 256 µm x 256 µm per biological group. *** p ≤ 0.001. Two-tailed unpaired Student's t test.  "
NCBI gene IDs:
"VE-cadherin: 12562"
Figure legend: "  Fig. 8: Enhanced collective cell migration in VE-cadherin-deficient lymphatic endothelial cells. (A) Collective cell migration of VE-cadherin-deficient and control LECs was assessed in wound scratch assays. LECs were grown up to four passages and seeded at a density of 5x104 cells on fibronectin-coated 8 well µ-chamber slides (growth area 0.22 cm2); scale bar 500 µm (B) The area of the zone denuded from cells is depicted in the curve diagram showing mean values ± SD of three individual experiments conducted in quadruplets. * p ≤ 0.01. Two-tailed unpaired Student's t test. Expanded View Figures - Legends  "
NCBI gene IDs:
"VE-cadherin: 12562"
Figure legend: "Micronuclei (indicated by arrow head) in GFP-NLS- or GFP-hcGAS expressing HEK293 cells before (0 h) or 24 h after γ-irradiation (IR; 10 Gy). Scale bar: 10 μm (A)."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "Micronuclei (indicated by arrow head) in GFP-NLS- or GFP-hcGAS expressing HEK293 cells before (0 h) or 24 h after γ-irradiation (IR; 10 Gy). The average MNs/cell. Graphs show Mean ± SEM (n=3 independent experiments) representing six different microscopic fields with over 200 cells."
NCBI gene IDs:
"GFP: 
cGAS: 214763"
Figure legend: "IFNB1 response in HEK293 cells stimulated with transfected plasmid DNA. Mean ± SEM of n=3 independent experiments."
NCBI gene IDs:
"IFNB1: 3456"
Figure legend: "Micronuclei (indicated by arrow head) and cGAS staining in WT and cGAS-/- BMDMos exposed to γ-irradiation (10 Gy). Scale bar: 10 μm."
NCBI gene IDs:
"cGAS: 214763"
Figure legend: "Cell death in WT and cGAS-/- BMDMos that were first synchronized at G2/M, then γ-irradiated (10 Gy) followed by release and analysis at indicated time points. Mean ±SD, x biological triplicates (n=3) per treatment group are shown."
NCBI gene IDs:
"cGAS: 214763"
Figure legend: "BMDMos from cGAS-/- mice exhibit enhanced DNA repair efficiency than those from WT and Sting-/- mice. (A) Representative comet tails of WT, cGAS-/- and Sting-/-BMDMos exposed to γ-irradiation (IR: 10 Gy) on ice then incubated at 37 ̊C for indicated duration. (B) Corresponding quantification of the comet tail moments from 20 different fields with n &gt; 200 comets of 3 independent experiments."
NCBI gene IDs:
"cGAS: 214763
Sting: 72512"
Figure legend: "cGAS promotes micronuclei generation in BMDMos independently of STING. Confocal microscopic visualization of micronuclei (indicated by arrow head) and cGAS staining in WT, cGAS-/- and Sting-/- BMDMos exposed to γ-irradiation (10Gy). Scale bar: 10 μm."
NCBI gene IDs:
"cGAS: 214763
Sting: 72512"
Figure legend: "γ-irradiation-induced cell death in WT, cGAS-/- BMDMos"
NCBI gene IDs:
"cGAS: 214763"
Figure legend: "γ-irradiation-induced cell death in Sting-/- and cGAS-/- Sting-/- BMDMos (F)."
NCBI gene IDs:
"cGAS: 214763
Sting: 72512"
Figure legend: "Bone marrow cells in WT (n=3) and cGAS-/- (n=3) mice 10 hours post γ-irradiation."
NCBI gene IDs:
"cGAS: 214763"
Figure legend: "Bone marrow cells in Sting-/- (n=3) and cGAS-/- Sting-/- (n=3) mice 10 hours post γ-irradiation."
NCBI gene IDs:
"cGAS: 214763
Sting: 72512"
Figure legend: "Obtained results showing enhanced HR efficiency upon knockdown of endogenous cGAS in U2OS cells. Immunoblot inserts in depict knockdown efficiency of cGAS and Histone H1.2."
NCBI gene IDs:
"cGAS: 115004
Histone H1.2: 3006"
Figure legend: "Results showing the effect of hcGAS, hcGAS△cGAMP, hcGAS△DNA or hcGAS△Oligo on HR in HEK293 cells. Corresponding immunoblot inserts depict cGAS expression."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "Results showing the effect of hcGAS, hcGAS△cGAMP, hcGAS△DNA or hcGAS△Oligo on NHEJ in HEK293 cells. Corresponding immunoblot inserts depict cGAS expression."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "cGAS is not recruited to DSB sites: Confocal microscopic images of GFP-NLS- or GFP-hcGAS-expressing U2OS-DSB reporter cells incubated (or not) with Shield-1 and 4-OHT to induce the expression and translocation of mCherry-LacI-FokI (red) to specific DSB sites. Scale bar: 10μm. The arrowheads indicate DSB sites."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "cGAS does not colocalize with γ-H2AX at DSB sites: GFP-NLS- or GFP-hcGAS- expressing HEK293 cells exposed (or not) to γ-irradiation (IR: 10 Gy) then stained for γ-H2AX. Scale bar: 10 μm."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "cGAS co-isolates with DNA repair proteins because of bound chromatin bridges. Nuclease digestion abrogates the co-isolation of cGAS and DNA repair proteins: Lysates of control (-IR) and γ-irradiated (+IR, 10 Gy, 30 min) GFP-hcGAS expressing HEK293 cells were treated (or not) with Benzonase before cGAS immunoprecipitation and analysis for indicated proteins. Agarose gel analysis of DNA in corresponding cell lysates in (E)."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "cGAS co-isolates with DNA repair proteins because of bound chromatin bridges. Co-isolation of cGAS and DNA repair proteins depends on its binding to DNA: cGAS pulldowns along with lysate inputs of control and γ-irradiated HEK293 cells expressing GFP-hcGAS or GFP-hcGASΔDNA probed for indicated proteins."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "Confocal images of γ-irradiated GFP-NLS-, GFP-hcGAS-expressing HEK293 cells stained for RAD51 (red) with or without γ-irradiation. Scale bar: 10 μm."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "Effect of indicated hcGAS variants on D-loop formation when pre-incubated with dsDNA. Percentage of D-loop formed in each reaction (left) graphed as the average of triplicates± SD."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "DR-GFP assay showing that hcGASΔDNA-Y215E is impaired in HR inhibition."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "hcGASΔDNA-Y215E but not hcGASΔcGAMP has a decreased affinity to dsDNA24."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "hcGASΔDNA-Y215E and hcGASΔcGAMP are defective in synthase activity."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "Negative-stain electron micrographs showing that hcGAScat-ΔDNA-Y215E is defective in inducing cGAS-dsDNA complexes. Scale bar: 100 nm."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "Effect of indicated hcGAS variants on D-loop formation."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "C. Representative images of DIV3 DRG neurons  nucleofected with a non-targeting siRNA (NT siRNA) or a siRNA against DLK  (DLK  siRNA), and stained for endogenous DLK  and β3-tubulin (TUBB3). On the right, quantification of the DLK  mean intensity in neurons  expressing control NT or DLK  siRNAs (n = 25 neurons/condition; Unpaired t test). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. Scale bar represents 10 μm in (C) 2 μm in zooms in (C)."
NCBI gene IDs:
"DLK: 26404"
Figure legend: "I-J. Representative  Western blots  of p-cJun and GAPDH  (I), and quantification of relative c-Jun phosphorylation  levels (J) from DIV6 cultured embryonic DRG neurons  overexpressing GFP  or NtermDLK-GFP, and subjected to NGF  withdrawal for 3h (n = 7-8 biological replicates; series of unpaired t tests /Mann-Whitney U tests followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "L. Representative images of HeLa  cells expressing the different HA-tagged wild-type (WT) and palmitoyl-mutant (CS) DLK  constructs (DLK-WT-ER, DLK-CS-ER, DLK-WT-CAAX, DLK-CS-CAAX, DLK-WT-MTS, DLK-CS-MTS), and stained  with markers of the  endoplasmic reticulum (KDEL), plasma membrane  (CellMask) and mitochondria  (cytochrome C). Data information: Scale bar represents 5 μm in (L)"
NCBI gene IDs:
"HA: 
DLK: 26404"
Figure legend: "M-N. Representative Western blots of DLK, p-cJun and GAPDH  (M) and quantification of relative  c-Jun phosphorylation  levels (N) from HeLa  cells transfected with HA  alone (HA) and the different HA-tagged wild-type (WT) and palmitoyl-mutant (CS) DLK  constructs (DLK-WT-ER, DLK-CS-ER, DLK-WT-CAAX, DLK-CS-CAAX, DLK-WT-MTS, DLK-CS-MTS) (n = 7-8 biological replicates; series of unpaired t tests /Mann-Whitney U tests with DLK-WT or DLK-CS followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"HA: 
DLK: 26404"
Figure legend: "Representative Western blots  of GFP, p-cJun and GAPDH  (B) from HeLa  cells non transfected (NT) or transfected with GFP  alone (GFP), GFP-tagged DLK  wild-type (DLK-WT-GFP), kinase dead mutant (DLK-KD-GFP), leucine zipper domain mutant (DLK-LZ-GFP) and a palmitoyl-site mutant (DLK-CS-GFP)"
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "quantification of relative c-Jun phosphorylation  levels (C) from HeLa  cells non transfected (NT) or transfected with GFP  alone (GFP), GFP-tagged DLK  wild-type (DLK-WT-GFP), kinase dead mutant (DLK-KD-GFP), leucine zipper domain mutant (DLK-LZ-GFP) and a palmitoyl-site mutant (DLK-CS-GFP) (n = 4-7 biological replicates; series of unpaired t tests /Mann-Whitney U tests with DLK-WT-GFP  followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 and ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "D. Representative images and zooms of HeLa cells expressing GFP  alone or the different GFP-tagged DLK  mutants. Data information: Scale bar represents 100 μm in (D) zooms in (D)."
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "E-F. Representative  Western blots of GFP, α-tubulin and Na+/K+-ATPase  from cytosolic  and membrane  fractions (E) and quantification of the percentage of DLK localized  in the membrane  and cytosolic  fraction (F) from HeLa  cells expressing GFP  alone or the different GFP-tagged DLK  mutants (n = 4-5 biological replicates; series of unpaired t tests /Mann-Whitney U tests with DLK-WT-GFP  followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 and ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "G-I. Representative stills (G), representative kymographs (H) and quantification of the number of GFP-positive particles (I) from time-lapse recordings of the different GFP-tagged DLK  mutants in DIV6 cultured embryonic DRG neurons (n = 31-65 neurons/condition; series of Mann-Whitney U tests with DLK-WT-GFP  followed by a Holm-Sidak correction). Data information: Arrowheads point to individual DLK-GFP  positive structures in G All graphs represent mean ± SEM. *p &lt; 0.05 and ***p &lt; 0.001. Scale bar represents 10 μm in (G) , 5 μm in (H)"
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "C. Representative images of HeLa  cells expressing HA   alone and the different HA-tagged DLK  constructs: a wild-type form of DLK  (DLK-WT-HA) and the palmitoyl-mutant DLK  (DLK-CS-HA). Data information: Scale bar represents 20 μm in (C)"
NCBI gene IDs:
"HA: 
DLK: 26404"
Figure legend: "D. Western blot analysis from total lysate (Input) and immunoprecipitates  (IP) of HA-tag protein immunoprecipitations  in HeLa  cells overexpressing HA  alone and the different HA-tagged DLK  constructs, DLK-WT-HA  and DLK-CS-HA  in the absence of any detergent."
NCBI gene IDs:
"HA: 
DLK: 26404"
Figure legend: "E-F. Lipidomic analysis of the immunoprecipitates  obtained from the HA-tagged protein immunoprecipitations  from extracts of HeLa  cells overexpressing HA  alone, DLK-WT-HA  and DLK-CS-HA. Quantification of lipid  classes (E) and sphingomyelin  fatty acid species (F) measured in the immunoprecipitates  (n = 3-4 independent experiments; series of unpaired t tests /Mann-Whitney U tests followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. CE, cholesteryl ester; CER, ceramide; DAG, diacylglycerol; DCER, dihydroceramides; HCER, hexosylceramides, LCER, lactosylceramides; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; SM, sphingomyelin; TAG, triacylglycerol."
NCBI gene IDs:
"HA: 
DLK: 26404"
Figure legend: "Representative stills (J) from  time-lapse recordings from 6DIV cultured embryonic DRG neurons  overexpressing GFP-tagged DLK  and treated with D609  for 3h (n = 29-46 neurons  Arrowheads in J point to individual DLK-GFP  positive structures. Data information: Scale bar represents 10 μm in (J)"
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "kymographs  and quantification of the number of DLK-GFP  positive particles (K) from  time-lapse recordings from 6DIV cultured embryonic DRG neurons  overexpressing GFP-tagged DLK  and treated with D609  for 3h (n = 29-46 neurons; Mann-Whitney U test). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. Scale bar represents , 5 μm in (K)"
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "Representative stills (C), kymographs (D) and quantification of the orientation (E) of DLK-GFP  positive particles from time-lapse recordings of DIV6 DRG neurons  overexpressing GFP-tagged DLK  and treated with dynasore  (DYN) or brefeldin A (BFA) for 3h (n = 34-101 neurons, Chi-square test in E Arrowheads in C point to individual DLK-GFP  positive structures. Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. Scale bar represents 10 μm in (C) and , 5 μm in (D)"
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "quantification of number of DLK-GFP  positive particles (F) from  time-lapse recordings of DIV6 DRG neurons  overexpressing GFP-tagged DLK  and treated with dynasore  (DYN) or  brefeldin A (BFA) for 3h (n = 34-101 neurons, series of Mann-Whitney tests with control followed by a Holm-Sidak correction in F). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
DLK: 26404"
Figure legend: "C. Representative images of DRG neurons  expressing GFP, Dnm-1-WT-HA  or Dnm1-DN-HA  form, cultured in the absence of NGF  for 8h, and stained  with antibodies against cCasp3  (magenta) and HA  tag (green). On the right, quantification of the ratio of cCasp3 positive cells over GFP  or HA  positive cells (n = 10-15 images from 3 independent cultures, series of unpaired t-test/Mann-Whitney U tests with control NGF  withdrawal followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. Scale bar represents 20 μm in (C)"
NCBI gene IDs:
"GFP: 
HA: 
Dnm-1: 13429
Dnm1: 13429"
Figure legend: "F. Representative images of axons  from cultured embryonic DRG neurons  expressing GFP, Dnm-1-WT-HA  or Dnm1-DN-HA  form and deprived of NGF   for 24h, and visualized with an antibody against neurofilament heavy chain (NFH). On the right, quantification of fragmented axons  using the  degeneration index in (n = 8-13 images from 3 independent cultures, series of unpaired t-test tests with control NGF   withdrawal followed by a Holm-Sidak correction). Data information: All graphs represent mean ± SEM. *p &lt; 0.05 **p &lt; 0.01 and ***p &lt; 0.001. Scale bar represents 40 μm in (F)"
NCBI gene IDs:
"GFP: 
HA: 
Dnm-1: 13429
Dnm1: 13429"
Figure legend: "(A) qRT-PCR analysis for Islr, Chd1 and Vim in total intestinal tissues, intestinal epithelium and lamina propria. n = 4."
NCBI gene IDs:
"Chd1: 12648
Islr: 26968
Vim: 22352"
Figure legend: "(C) qRT-PCR analysis for Islr in CD45+, CD45-Epcam+, CD45-Epcam-CD90+ and CD45-Epcam-CD90- cells sorted from intestinal tissues. n = 3 technical replicates."
NCBI gene IDs:
"Islr: 26968"
Figure legend: "(D) In situ hybridization for Islr with RNAscope probe in intestine and colon from 8-week-old mice, showing that Islr is primarily expressed in stromal cells. Negative and positive controls were shown in Appendix Figure S1A. Scale bar: 25 μm."
NCBI gene IDs:
"Islr: 26968"
Figure legend: "(E) In situ hybridization for ISLR with RNAscope probe in human mucosa, inflamed mucosa from CD and UC patients. Scale bar: 25 μm."
NCBI gene IDs:
"ISLR: 3671"
Figure legend: "(F) qRT-PCR analysis showing the dynamic changes of Islr in colonic tissues from mice upon DSS treatment and after DSS removal. n = 4 at each time points."
NCBI gene IDs:
"Islr: 26968"
Figure legend: "(G) In situ hybridization for Islr with RNAscope probe in mouse colons without or with 3-day or 5-day DSS treatments. Scale bar: 50 μm."
NCBI gene IDs:
"Islr: 26968"
Figure legend: "(H) The cancer genome atlas (TCGA) RNA-seq analysis showing that ISLR is upregulated in human rectal adenocarcinoma relative to normal rectal tissues. The horizontal lines between the box limits represent the median, the box limits indicate the interquartile ranges and the whiskers indicate limit superior and limit inferior respectively. n = 65."
NCBI gene IDs:
"ISLR: 3671"
Figure legend: "(J) ISLR level increased with nodal metastasis in colorectal cancer patients. N0, no regional lymph node metastasis; N1, metastases in 1 to 3 axillary lymph nodes; N2, metastases in 4 to 9 axillary lymph nodes; N3, metastases in 10 or more axillary lymph nodes. The solid horizontal lines represent the median, the box limits indicate the interquartile ranges and the whiskers indicate limit superior and limit inferior respectively."
NCBI gene IDs:
"ISLR: 26968"
Figure legend: "(K In situ hybridization for ISLR/Islr with RNAscope probes in normal human colon, human malignant adenocarcinoma, metastatic adenocarcinoma (K), t indicating tumor; a indicating adjacent tissues of tumor. Scale bar: 50 μm."
NCBI gene IDs:
"ISLR: 26968
Islr: 26968"
Figure legend: "L) In situ hybridization for ISLR/Islr with RNAscope probes in mouse colon tumors from AOM-DSS model (L). t indicating tumor; a indicating adjacent tissues of tumor. Scale bar: 50 μm."
NCBI gene IDs:
"ISLR: 26968
Islr: 26968"
Figure legend: "(B) qRT-PCR analysis for Ets1 in colonic epithelium and lamina propria. n = 3."
NCBI gene IDs:
"Ets1: 23871"
Figure legend: "(D) qRT-PCR for Ets1 in colons with or without 5-day DSS treatment. n = 4."
NCBI gene IDs:
"Ets1: 23871"
Figure legend: "(F) Pearson correlation analysis on ETS1 and ISLR in colorectal adenocarcinoma TCGA RNA-seq (P &lt; 0.001; R = 0.68)."
NCBI gene IDs:
"ETS1: 2113
ISLR: 3671"
Figure legend: "(G) Western blotting for ISLR and HA in lysates of UC-MSCs transfected with pCMV5-HA or pCMV5-ETS1. α-Tubulin was used as a loading control."
NCBI gene IDs:
"HA: 
ETS1: 23871"
Figure legend: "(H) Luciferase activity in lysates of UC-MSCs transfected with luciferase reporter plasmids of pGL3-basic empty vector (basic), wild type ISLR promoter or mutant promoter with mutation of ETS1 binding sites under normal and ETS1 overexpression conditions. n = 3."
NCBI gene IDs:
"luciferase: 
ETS1: 23871
ISLR: 26968"
Figure legend: "(I) Chromatin immunoprecipitation assay was carried out on UC-MSCs cells using antibodies against ETS1 and Histone H3. The antibody against Histone H3 was used as a positive control. IgG was used as a negative control. The enrichment of ETS1 binding to ISLR promoter was quantified using qPCR. n = 3 technical replicates."
NCBI gene IDs:
"ETS1: 23871
ISLR: 26968"
Figure legend: "(J) Chromatin immunoprecipitation assay was carried out on primary intestinal stromal cells isolated from WT mice without DSS treatment or WT mice after 5-day DSS treatment using antibodies against ETS1 and Histone H3. The antibody against Histone H3 was used as a positive control. IgG was used as a negative control. The enrichment of Ets1 binding to Islr promoter was quantified using qPCR. n = 3 technical replicates."
NCBI gene IDs:
"Islr: 26968"
Figure legend: "(H) The growth curve of HCT116 colorectal cancer cells cultured in the supernatant from WT or cKO IMCs, concomitantly transfected with PCDH empty vector or PCDH-YAP1-5SA vector. n = 4 technical replicates."
NCBI gene IDs:
"YAP1: 10413"
Figure legend: "(I) Pearson correlation analysis of ISLR and CTGF (P &lt; 0.001; R = 0.69), ISLR and FSTL1 (P &lt; 0.001; R = 0.81), as well as ISLR and CYR61 (P &lt; 0.001; R = 0.65) in human colorectal cancer based on TCGA RNA-seq database."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490
FSTL1: 11167
ISLR: 3671"
Figure legend: "(A) Schematics of culture epithelial cells with supernatant from HEK293FT cells transfected with pcDNA3.1 empty vector or pcDNA3.1-hISLR-HA plasmids. Western blotting for HA in the supernatant from HEK293FT cells transfected with the indicated plasmids."
NCBI gene IDs:
"hISLR: 3671"
Figure legend: "(E) Western blotting for HA and ISLR in the supernatant from NCM460 colonic epithelial cells with pcDNA3.1 empty vector or pcDNA3.1-hISLR-HA plasmids. Western blotting for pMST1/2, MST1, pMOB1 and MOB1, pYAP1 and YAP1 in lysates from NCM460 CRC cells cultured in the supernatants with or without hISLR-HA. β-actin was used as a loading control."
NCBI gene IDs:
"HA: 
hISLR: 3671"
Figure legend: "(H) qRT-PCR for CTGF and CYR61 in HEK293FT cells cultured in the supernatant with or without hISLR-HA. n=3."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490"
Figure legend: "(A) Western blotting for Islr in the supernatant from primary cultured intestinal epithelial cells (IECs) transfected with pcDNA3.1 or pcDNA3.1-mIslr-HA plasmids, and western blotting for pMst1/2, Mst1, pMob1 and Mob1, pYap1 and Yap1 in lysates from primary IECs cultured in the supernatants with or without mIslr-HA. β-actin was used as a loading control."
NCBI gene IDs:
"HA: 
mIslr: 26968"
Figure legend: "(C) qRT-PCR for Ctgf, Cry61 and Fstl1 in primary intestinal epithelial cells cultured in the supernatant with or without mIslr for 24 hours. n=4."
NCBI gene IDs:
"Cry61: 16007
Ctgf: 14219
Fstl1: 14314"
Figure legend: "(D) Activity of TEAD-Luciferase reporter in primary intestinal epithelial cells upon mIslr, Yap1 or mIslr and Yap1. n=4."
NCBI gene IDs:
"Luciferase: 
TEAD: 7004"
Figure legend: "(F) qRT-PCR for Ctgf and Cry61 in primary intestinal epithelial cells 48 hours after treatments of mIslr or/and Yap1 siRNA (siYap1). n=4."
NCBI gene IDs:
"Cry61: 16007
Ctgf: 14219
Yap1: 22601"
Figure legend: "(I) qRT-PCR for Ctgf, Cry61 and Fstl1 in primary intestinal organoids 24 hours after the treatments. n=3."
NCBI gene IDs:
"Cry61: 16007
Ctgf: 14219
Fstl1: 14314"
Figure legend: "(A) Analysis of the single-cell transcriptomics dataset for the mouse visual cortex (Tasic et al, 2016) revealed that the expression of the metabolism-associated gene Cox6a2 is highly restricted to PV+ interneurons. Cox6a2 is expressed in all subtypes of PV+ interneurons (marked in blue color) but rarely detected in other neuronal subtypes from the mouse visual cortex. Cox6a1, which encodes the other protein isoform of COX6A, is expressed in all subtypes."
NCBI gene IDs:
"Cox6a1: 12861
Cox6a2: 12862"
Figure legend: "(B RT-PCR showing expression of Cox6a2 in the brain and heart."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(A) Images showing absence of COX6A2 immunoreactivity in the cortex of Cox6a2-/- mice."
NCBI gene IDs:
"Cox6a2: 12862"