EMBO/SourceData huggingface dataset:
Figure legend: "B) Endogenous Ptc subcellular localization after endocytosis inhibition by the dorsal expression of a dominant negative form of Rab5. The top panel is a schematic representation of the localized expression. The left panel is a digital 3D reconstruction and the right panel shows an apical and basal confocal sections. that accumulation of Ptc occurs at both the apical and basal sides of the dorsal part of the disc, compared to the wild type control on the ventral side."
NCBI gene IDs:
"Rab5: 33418"
Figure legend: "accumulation of Ptc occurs at both the apical and basal sides of the dorsal part of the disc, compared to the wild type control on the ventral side. C) Digital 3D reconstruction (left) and apical and basal confocal sections (right) of endogenous Ptc localization after endocytosis inhibition by the dorsal expression of a dominant negative form of shibire."
NCBI gene IDs:
"shibire: 45928"
Figure legend: "A) 3D reconstructions of immuno-labelled endogenous Ptc of wing discs in wild type condition and after RNAi expression for different ESCRT complex components. All RNAis' expression result in Ptc accumulation in large structures, located apically under Hrs (ESCRT-0 component) inhibition, and basally under inhibition of the ESCRT components I (Tsg101), II (Vps22) and III (Shrub). Graphs depicting signal values from apical to basal show a shift of higher intensity curves either towards acute apical or basal accumulation."
NCBI gene IDs:
"Hrs: 33458
Shrub: 35933
Vps22: 11267
Tsg101: 39881"
Figure legend: "B) Detailed confocal imaging of immuno-labelled endogenous Ptc localization after clonal Tsg101 down-regulation. The image shows abnormal accumulation of Ptc clusters in basal cytonemes emanating from receiving cells (arrows), since in wild type conditions Ptc visualization at basal membranes is not possible due to its rapid internalization and processing after Hh reception"
NCBI gene IDs:
"Tsg101: 39881"
Figure legend: "C) Ptc accumulation in these punctated structures (arrows) is also visualized on receiving cytonemes stabilized with the co-receptor Ihog-RFP and after Tsg101 inhibition."
NCBI gene IDs:
"Tsg101: 39881"
Figure legend: "D) 3D reconstruction of Hh producing cytoneme network stabilized by Ihog-RFP shows that Ptc accumulation after Tsg101 treatment localizes above the signal sending cytoneme extensions (arrow)."
NCBI gene IDs:
"Tsg101: 39881"
Figure legend: "A) Confocal image of wing disc expressing the human tetraspanin CD63-GFP expressed in the Hh receiving cells colocalizing with Ptc (Bac.Ptc-cherry) in vesicle-like structures (yellow arrows) along basal cytonemes."
NCBI gene IDs:
"cherry: 
Ptc: 35851"
Figure legend: "B) In vivo confocal frames of abdominal histoblasts showing colocalization of CD63-Cherry and Ptc (Yac.Ptc-GFP) at punctae moving in anterograde direction along cytonemes (white arrowheads show trajectory in time-lapse frames)."
NCBI gene IDs:
"GFP: 
Ptc: 35851"
Figure legend: "G) Confocal imaging of receiving cells expressing the HA tagged Tsp96F construct also shows co-localization with endogenous Ptc at cytonemes (yellow arrows indicate Tsp96F-HA and Ptc co-localizing punctate structures)."
NCBI gene IDs:
"HA: 
Tsp96F: 43127"
Figure legend: "H) Western blot against HA-tag of Immuno-Precipitated sample (IP), Depleted Sample (Dep), High Speed Sample (HSS) Whole Sample and Control sample from a co-immunoprecipitation experiment isolating GFP of Tsp96F-HA and Ptc-GFP expressing larvae or GFP and Tsp96F-HA expressing larvae as negative control. Arrowhead indicates the positive band corresponding to Tsp96F-HA expected molecular weight (30-40 kDa), present in all samples including the immuno-precipitated one and not present in the negative control sample."
NCBI gene IDs:
"GFP: 
HA: 
Tsp96F: 43127"
Figure legend: "E) Effect on EV-associated Ptc secretion after gene silencing on Cl8 cells by dsRNAs against the SNARE proteins Syb and α-Snap, the tetraspanin Tsp96F and the ESCRT components Hrs, Tsg101, Shrub and Vps4. Hsp70 in cells and EVs is detected by different exposition time."
NCBI gene IDs:
"α-Snap: 40233
Hrs: 33458
Shrub: 35933
Syb: 36080
Tsg101: 39881
tetraspanin: 43127
Tsp96F: 43127
Vps4: 32777"
Figure legend: "A) Projection images of three confocal sections (1 μm) at the basal side of wing discs expressing the Bac constructs for Hh-GFP and Ptc-cherry, used to analyse co-localization between the ligand and the receptor in wild type conditions and after inhibition of vesicle fusion (Syb RNAi), vesicle trafficking (Hrs RNAi) or Ptc protein degradation (Dor RNAi). Boxplot shows co-localization rates using the Manders coefficient for proportion of red covered by green in projected images for wing discs temporally expressing RNAi against ESCRT proteins Hrs and Vps4, SNAREs Syb and α-Snap, and Dor as control for effects after Ptc degradation-blocking, as well as temporally over-expressing the Tsp96F-HA. Central horizontal lines show median values of N=8-12 box shows lower and upper quartiles and the whiskers show the maximum and minimum excluding. Note the significant reduction in co-localization rates (1= total coverage of red by green) after RNAi expression for both ESCRT and SNARE proteins as well as after Tsp96F-HA over-expression, while RNAi expression for Dor presents greater co-localization."
NCBI gene IDs:
"Bac: 
HA: 
α-Snap: 40233
Dor: 38543
Hrs: 33458
Syb: 36080
Tsp96F: 43127
Vps4: 32777"
Figure legend: "B) Projection images of three confocal sections (1 μm) from the apical side of the wing discs. Quantification of co-localization rates using apical projection images; central horizontal lines show median values of N=8-12 , box shows lower and upper quartiles and the whiskers show the maximum and minimum excluding outliers. Note that at the apical side no clear significant reduction in co-localization rates (1= total coverage of red by green) is observed after RNAi expression or in Tsp96F-HA over-expression."
NCBI gene IDs:
"HA: 
Tsp96F: 43127"
Figure legend: "C) Correlation coefficients between the maximum value of quantified integrated density (integrating both fluorescence intensity and signal area) for Ptc-GFP values (Yac construct) and distance from the basal side (-1=basal, +1=apical). Box plot shows quantification data of discs after RNAi expression for the vacuole sorting protein Dor (necessary for Ptc degradation), the ESCRT Hrs, the SNARE Syb as well as after over-expression of the Tsp96F-HA construct and wild type condition. Central horizontal lines show median values of N=8-12, box shows lower and upper quartiles and the whiskers show the maximum and minimum excluding outliers. Note the apical localization of Ptc-GFP highest values after degradation impairment (Dor RNAi) in contrast to the basal shift resulting from exocytosis deficiency (Syb RNAi)."
NCBI gene IDs:
"HA: 
Dor: 38543
Hrs: 33458
Syb: 36080
Tsp96F: 43127"
Figure legend: "A) Confocal images at the lateral plane showing the immunostaining of the medium threshold Hh target Collier (Col) of wild type wing discs and discs after inhibition of vesicle fusion (α-Snap RNAi), vesicle trafficking (Hrs RNAi) or in over-expression of the Tsp96F-HA. Quantification of gradient lengths (μm) using Collier signal and normalized to the wing pouch size, showing the proportion of wing pouch covered by signal (1=total coverage). Quantification was performed for wild type condition as well as in temporally regulated RNAi expression for the ESCRT complex proteins Hrs, Tsg101, Shrub and Vps4 and for the SNARE proteins Syb, Snap24, α-Snap, as well as in transient over-expression of the Tsp96F-HA. Central horizontal lines show median values of N=7-22 , box shows lower and upper quartiles and the whiskers show the maximum and minimum excluding outliers. Note a slight but significant increase in the length of Col signal for all treatment compared to the wild type condition."
NCBI gene IDs:
"HA: 
α-Snap: 40233
Hrs: 33458
Shrub: 35933
Snap24: 41193
Syb: 36080
Tsg101: 39881
Tsp96F: 43127
Vps4: 32777"
Figure legend: "B) Lateral confocal images and quantification of the extension of immuno-labeled Engrailed as the high threshold Hh target. Quantification shows number of cell diameters covered by signal from the posterior/anterior border marked by Hh-GFP (Bac construct); central horizontal lines show median values of N=6-10 box shows lower and upper quartiles and the whiskers show the maximum and minimum excluding outliers. Note a significant reduction after RNAi expression, suggesting Hh reception impairment and flattening of the signal gradient."
NCBI gene IDs:
"Hh: 42737"
Figure legend: "C) Lateral confocal images and quantification of Ptc transcription as a Hh target through immunolabeling of the reporter β−galactosidase (Ptc-LacZ). Middle graph shows the extension covered by signal normalized to wing pouch size, in wild type condition (N=6) or after ectopic expression of the Tsp96F-HA construct (N=9). Green shaded areas show data variability and dotted line gives the average values. Note the greater distance covered after Tsp96F-HA transient expression. Boxplot (right) shows distance quantifications with central horizontal lines showing median values of N=6-9 , box showing lower and upper quartiles and whiskers showing the maximum and minimum excluding outliers. Note a significant extension under Tsp96F-HA expression, suggesting again flattening of the signal gradient."
NCBI gene IDs:
"HA: 
LacZ: 
β−galactosidase: 
Ptc: 35851
Tsp96F: 43127"
Figure legend: "(B, C) (B) Growth inhibition  assay of  E. coli DH10B  cells harbouring pTrc200 vector or each of its derivatives expressing Tde1  variants with IPTG  induction. (C) Growth inhibition assay of E. coli  DH10B   cells co-expressing the Tde1 variants expressed from pTrc200 plasmid and Tdi1 immunity gene expressed from pRL662 plasmid. Growth  curve was determined at OD600. Graphs of panels B and C show mean ± SD of three biological replicates (n=3), each averaged with 3 technical repeats. One way ANOVA was used for the analysis of statistical significance followed by the Tukey's multiple comparison. Different letters indicate statistically different groups of strains (p value, 4.6x10-5 and 5.19x10-8 for panels B and C respectively)."
NCBI gene IDs:
"Tde1: 
Tdi1: "
Figure legend: "(A) Growth inhibition assay of E. coli DH10B  cells harbouring pTrc200 vector or each of its derivatives expressing Tde1  variants with IPTG  inducible expression. The growth  of  E. coli  was monitored by CFU counting every 1 hr. Data information: Graphs of panels A, show mean ± SD of three biological replicates (n=3), each averaged with 3 technical repeats. One way ANOVA was used for the analysis of statistical significance followed by the Tukey's multiple comparison."
NCBI gene IDs:
"Tde1: "
Figure legend: "For  membrane permeabilization  assays, BW25113  WT alone or ∆lacY(pYTA-lacZ) cells harbouring pTrc200 vector or each of its derivatives expressing Tde1  variants were carried out for (C) propidium iodide permeability   with cells treated with Propidium iodide  and Hoechst  for detection by fluorescence microscope (Scale bar = 5 μm). For quantification of cells with PI  signals, a total of 6 randomly selected images obtained from two biological repeats were used to quantify the number of PI-stained  cells / number of Hoechst-stained  cells as indicated."
NCBI gene IDs:
"lacY: 
Tde1: 
lacZ: 945006"
Figure legend: "(D) Bacteriostatic activity assay. E. coli DH10B  cells harbouring pTrc200 vector or each of its derivatives expressing Tde1  variants were cultured with or without IPTG  induction for 1 hr. The IPTG-induced cells were further centrifuged and resuspended in the fresh medium with or without IPTG. Cell density was measured again before continuous growth  for additional 1 hr. Data information: Graphs of panels show mean ± SD of three biological replicates (n=3), each averaged with 3 technical repeats. One way ANOVA was used for the analysis of statistical significance followed by the Tukey's multiple comparison. Different letters indicate statistically different groups of strains (p value, 1×10-16 and 2×10-16 for panels D respectively)."
NCBI gene IDs:
"Tde1: "
Figure legend: "(A) Co-immunoprecipitation (Co-IP) in A. tumefaciens. A. tumefaciens C58 ∆tde1 harbouring pTrc200 vector or its derivatives expressing HA-tagged Tde1  variants. Anti-HA  resin was used to coprecipitate the Tde1  variants and Tap1."
NCBI gene IDs:
"HA: 
tde1: 
Tde1: "
Figure legend: "(B) Secretion assay for HA-tagged Tde1  variants. Western blot  for the cellular  and extracellular  fractions of A. tumefaciens C58 ∆tdei and ∆tdei∆tssK expressing the HA-tagged Tde1  variants. Hcp  secretion was detected as a positive control for active T6SS  secretion. Representative western blot results of three biological repeats were shown with antibody against HA, Hcp, or EF-Tu where EF-Tu serve as a loading and non-secreted protein control. Protein markers are indicated in kDa."
NCBI gene IDs:
"HA: 
Tde1: 
tssK: "
Figure legend: "(A)  Fluorescence microscopy for Tde1 translocation.  A. tumefaciens C58 ∆tdei  expressing Tde1  variants fused with sfGFP  (in green) and  E. coli DH10B  carrying mCherry  (false coloured in blue) were co-cultured for 20 hrs. A cyan fluorescence with merged blue and green signals represented the translocation  of Tde1  variants from  A. tumefaciens  to  E. coli (Scale bar = 5 μm)."
NCBI gene IDs:
"mCherry: 
sfGFP: 
Tde1: "
Figure legend: "(A) Interbacterial competition of A. tumefaciens C58 ∆tdei and ∆tdei∆tssK expressing the Tde1  variants against E. coli cells was carried out on LB medium and E. coli survival rate was quantified by CFU counting. (B) Interbacterial competition  between various A. tumefaciens C58  strains and A. tumefaciens 1D1609 on AK medium and the competition outcome was shown by competitive index. Data information: Data in panel A are mean ± SD of four biological repeats of two independent experiments (n=4). Panels B show mean ± SD of three biological repeats (n=3). One way ANOVA was used for the analysis of statistical significance followed by the Fishers Least Significant Difference (LSD) test for panel A and B Different letters indicate statistically different groups of strains (p value, 3.63×10-4, 2.70 × 10-3, 2.3 × 10-15 for panels A, B respectively)."
NCBI gene IDs:
"Tde1: 
tssK: "
Figure legend: "(C) Secretion assay for Tde1  and its variants co-expressed with its immunity protein Tdi1  in  A. tumefaciens C58 ∆tdei and ∆tdei∆tssK. Data information:  Western blots were detected with specific antibody against Tde1, Hcp, or EF-Tu serving as a loading and non-secreted protein control. Protein markers are indicated in kDa. Results in panel C are representative of three biological repeats."
NCBI gene IDs:
"Tde1: 
tssK: "
Figure legend: "(C) The upregulation of NOD2 gene expression was assessed by quantitative PCR. The results are depicted as the mean fold change in NOD2 gene expression relative to unstimulated cells (n = 11)."
NCBI gene IDs:
"NOD2: 64127"
Figure legend: "LL37 and IRGM gene expression is upregulated following NOD2 activation (A‐D). Alveolar macrophages were incubated in the presence of 10 μg/mL of MDP or 100 ng/mL of LPS for 24 h. Upregulation of (A) LL37 and (D) IRGM gene expression was assessed after specific ligand recognition by quantitative PCR using the Taqman system and the ΔΔCT method for relative quantification. The fold change in gene expression relative to unstimulated cells is depicted. Data are representative of two independent experiments (n = 7-12). Bold lines indicate median values."
NCBI gene IDs:
"LL37: 820
IRGM: 345611
NOD2: 64127"
Figure legend: "(E) LL37 and (F) IRGM gene expression was assessed by quantitative PCR using the Taqman system and the ΔΔCT method for relative quantification. Fold changes in gene expression relative to unstimulated cells are reported. Bold lines indicate median values of n = 9. *p 0.05 and **p 0.01 using the two‐tailed Wilcoxon signed‐rank test."
NCBI gene IDs:
"LL37: 820
IRGM: 345611"
Figure legend: "mRNA expression of individual patients normalized to 18S-rRNA and relative to a pooled organ donor sample. Lines between dots connect samples from the same patient. CP: chronic pancreatitis; PDAC: pancreatic ductal adenocarcinoma; significant difference (P &lt; 0.05) between tumor and normal tissue P = 9.39 × 10−5 determined by paired two-tailed Student's t-test, n = 17 patients."
NCBI gene IDs:
"18S: "
Figure legend: "Association of microarray-based TBL1 expression and survival in 105 patients with PDAC: High TBL1 expression is significantly associated with shorter post-resection survival. P-value determined by log-rank test as described in Materials and Methods."
NCBI gene IDs:
"TBL1: 6907///90665"
Figure legend: "Immunohistochemistrystaining for TBL1 in paraffin-embedded tissue sections of wild-type or p48+/Cre; Kras+/LSL-G12Dmice. 1, 2: healthy tissue with acini, islet of Langerhans, and duct (zoom-in in panel 2) showing occasional weak nuclearstaining. 3, 4: PanIN-1 lesions with nuclearstaining. 5, 6: PanIN-2 lesions with nuclearstaining. 7, 8: PanIN-3 lesion with nuclearstaining. 9-12: invasive pancreatic ductal adenocarcinoma cells with nuclear and occasional faint cytoplasmicstaining. Scale bars: 100 μm. Images are representative of 32 wild-type and 29 p48+/Cre; Kras+/LSL-G12Dmice."
NCBI gene IDs:
"p48: 16391
Kras: 16653"
Figure legend: "Left panel: EdU incorporation in siRNA-transfected Capan-1 cells (72 h post-transfection), n = 6 cell culture wells per group; significantly different (P &lt; 0.05) from control (no siRNA) *, siNC #1 #, siNC #2 $; P-values: siTBL1#1 8.24 × 10−4 (*), 5.43 × 10−4 (#), 7.93 × 10−6 ($); siTBL1 #2 1.66 × 10−11 (*), 1.27 × 10−11 (#), 8.79 × 10−13 ($), one-way ANOVA with Bonferroni post-test. Right panel: EdU incorporation of Panc02 cells with stable expression of shRNA; n = 6 cell culture wells per group; significantly different (P &lt; 0.05) from control (shNC) #P = 0.0398; Welch's t-test."
NCBI gene IDs:
"TBL1: 6907///90665"
Figure legend: "Cellgrowth of siRNA-transfected Capan-1 or Panc8680 cells; n = 3 cell culture wells per group; significantly different (P &lt; 0.05) from control (no siRNA) *, siNC #1 #, siNC #2 $; P-values: Capan-1 cells 48 h time-point: 0.0408 (*) for siTBL1 #1 / 0.0464 for siTBL1 #2, Capan-1 cells 72 h time-point: 8.60 × 10−5 (*), 1.82 × 10−3 (#), 3.24 × 10−5 ($) for siTBL1 #1 / 2.39 × 10−10 (*), 3.34 × 10−9 (#), 1.08 × 10−10 ($) for siTBL1 #2; Panc8680 72 h time-point: 1.55 × 10−4 (*), 2.73 × 10−5 ($); two-way ANOVA with Bonferroni post-test."
NCBI gene IDs:
"TBL1: 21372
TBL1: 6907///90665"
Figure legend: "Chromatin immunoprecipitation from Capan-1 cells with or without siRNA-mediated knockdown of TBL1. IgG and primers for PIK3CA coding region served as a negative control; histone H3 served as a positive control."
NCBI gene IDs:
"PIK3CA: 5290
TBL1: 6907///90665"
Figure legend: "Proliferation time course assay in control and TBL1 shRNA-transfected Panc02 cells overexpressing constitutively active PI3K p100α mutant E545K. Cells were stained with crystal violet, and absorbance was measured at 595 nm. n = 9 cell culture wells per group; statistically significant at P &lt; 0.05 for indicated comparisons: shNC pBabe-puro vs. shNC pBabe-PI3K (*), shTBL1 pBabe-puro vs. sbTBL1 pBabe-PI3K (#), shNC pBabe-puro vs. shTBL1 pBabe-puro ($), shNC pBabe-PI3K vs. shTBL1 pBabe-PI3K (†); P-values: day 2: 2.01 × 10−3 (#), 4.11 × 10−9 ($), 5.77 × 10−3 (†); day 3: 4.50 × 10−5 (*), 1.01 × 10−9 (#), 1.71 × 10−30 ($), 1.22 × 10−6 (†); day 4: 5.61 × 10−22 (*), 1.10 × 10−45 (#), 6.65 × 10−62 ($); two-way ANOVA with Bonferroni post-test."
NCBI gene IDs:
"PI3K: 18708
TBL1: 21372"
Figure legend: "mRNA expression of PIK3CA in human patient samples; significant difference (P &lt; 0.05) between tumor and normal tissue *P = 2.13 × 10−4 determined by paired two-tailed Student's t-test, n = 18 patients."
NCBI gene IDs:
"PIK3CA: 5290"
Figure legend: "Correlation of mRNA expression of TBL1 and PIK3CA in human patient tumor tissue (normalized to 18S rRNA and relative to a pooled organ donor sample); n = 17 patients; P-value determined by testing for slope of regression line ≠ 0."
NCBI gene IDs:
"18S: 
PIK3CA: 5290
TBL1: 90665///6907"
Figure legend: "Survival analysis in 105 patients with PDAC: High PIK3CA expression is significantly associated with shorter post-resection survival. P-value determined by log-rank test as described in Materials and Methods."
NCBI gene IDs:
"PIK3CA: 5290"
Figure legend: "A-D Panc02 cells with stable expression of luciferase were implanted subcutaneously into C57Bl6/N mice. Six days later, tumors were injected three times per week with 108 ifu of adenovirus encoding shRNA. (A-C): Data plotted as mean ± SEM; n = 4 animals in control (shNC) group and n = 3 animals in knockdown (shTBL1) group; significantly different (P &lt; 0.05) between control (shNC) and knockdown (shTBL1) *. (A) Tumor volume determined by transcutaneous measurement with digital calipers; P-values: 9.21 × 10−3 at day 15, 6.02 × 10−4 at day 18, 2.49 × 10−4 at day 20; two-way ANOVA with Bonferroni post-test. (B) Luminescence of tumors after intraperitoneal injection of firefly d-luciferin; P = 0.0133 at day 20; two-way ANOVA with Bonferroni post-test. (C) Tumor mass at necropsy; P = 0.0299, one-sided Welch's t-test. (D) Luminescence images of one representative mouse in each group."
NCBI gene IDs:
"TBL1: 21372"
Figure legend: "TBL1-depletion leads to reduction in PI3 kinase p110α and cell cycle-associated proteinsPanc02 cells with stable expression of shRNA were implanted subcutaneously into C57Bl6/Nmice. Seven days later, mice were treated with 20 mg/kg gemcitabine delivered by intraperitoneal injection. After 21 days, mice were sacrificed, tumors were removed and proteins were extracted and immunoblotted."
NCBI gene IDs:
"TBL1: 21372"
Figure legend: "A-D Appearance of autophagic structures (marked with GFP-Atg8a) during wound closure in the epidermis of control third instar larvae and after epidermal knockdown of the autophagy pathway components Atg1, Atg5, Atg6, Atg7 or Atg12. All constructs are expressed in the epidermis under the control of the A58-Gal4 driver. (A) Time points from movies of wounded epidermis. The wounds have closed by 2 h in all cases. (B) Higher magnification of the areas marked by magenta boxes at t=120 min. (C) Quantification of the appearance of GFP-Atg8a puncta in the imaged area (10000 µm2) 3 control larvae, shown for each individual larva. (D) Quantification of number of GFP-Atg8a puncta in different genetic conditions measured in an area of 10000 µm2 at the time of wound closure; n=4-10 larvae each genotype, for the detail see Data analysis."
NCBI gene IDs:
"A58: 2845988
Atg1: 39454
Atg12: 39383
Atg5: 31666
Atg6: 42850
Atg7: 37141
Gal4: 855828"
Figure legend: "Control of epidermal autophagy by TOR signalling. (A) Epidermis of third instar larvae expressing the autophagosome marker GFP-Atg8a together with constructs for up- or down-regulating the autophagy pathway in the epidermis. Healthy epidermis contains few autophagosomes, but artificially activating autophagy through overexpression of Atg1 or blocking TOR signalling leads to accumulation of autophagosomes. (B) Higher magnification of the areas marked by magenta boxes in (A)."
NCBI gene IDs:
"Atg1: 39454
TOR: 47396"
Figure legend: "A-D Fluorescence loss in photobleaching (FLIP) to test free cytoplasmic GFP motility within the epidermis. A small area (magenta circle; 179 or 1098 µm2) was laser-illuminated for the indicated times (3 or 20 min) in control or Atg1-expressing epidermis also expressing free GFP. (A) Snapshots before bleaching and at 3 points of recovery. (B) Kymographs along the broken line in (A) during recovery. (C, D) Quantification of fluorescence recovery after bleaching, shown separately for 3 and 20 min bleaching protocols."
NCBI gene IDs:
"Atg1: 39454"
Figure legend: "B-D Atg1-induced syncytium formation is abolished when Atg5 function is downregulated (compare to rows 2 and 3 in Fig. 5A, B); same representation as shown in Fig. 5A-D."
NCBI gene IDs:
"Atg1: 39454
Atg5: 31666"