EMBO/SourceData huggingface dataset:
Figure legend: "(B) iMEFgt/gt were co-transfected with expression plasmids for V5-tagged m152 together with either Cherry-STING or ev in combination with cGAS-GFP (stimulated) or ev (unstimulated). 24 hours post transfection cells were fixed for immunolabeling with an anti-V5 antibody. White boxes indicate the region shown at a higher magnification. Scale bar represents 10 µm."
NCBI gene IDs:
"Cherry: 
GFP: 
m152: 
V5: 
cGAS: 115004
STING: 72512"
Figure legend: "(C) Lysates of Cherry-STING and either CD4-V5 or m152-V5 expressing 293T cells were subjected to immunoprecipitation (IP) with an anti-V5 antibody. Input and IP samples were analyzed by IB with the indicated antibodies. Data information: IB shown are representative of three (C) or two (D) independent experiments."
NCBI gene IDs:
"Cherry: 
m152: 
V5: 
CD4: 12504
STING: 72512"
Figure legend: "(D) iMEF stably expressing ev or m152-V5 were left unstimulated or stimulated with 10 µg/ml ISD and lysed 90 minutes later. m152 was immunoprecipitated with an anti-V5 antibody and samples were analyzed by IB with V5, STING and phospho-TBK1 (pTBK1) specific antibodies. Data information: IB shown are representative of three (C) or two (D) independent experiments."
NCBI gene IDs:
"m152: 
V5: "
Figure legend: "(B) Cherry-STING, IFNβ-Luc, pRL-TK, cGAS-GFP (stimulated) or IRES-GFP (unstimulated) and indicated expression plasmids as shown in (A) were transiently expressed in 293T cells and a dual luciferase assay was performed. Data is combined from two out of three independent experiments and shown as mean ± SD. Data information: Student's t-test (unpaired, two-tailed), n.s. not significant, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001"
NCBI gene IDs:
"Cherry: 
GFP: 
Luc: 
pRL: 
TK: 
cGAS: 115004
IFNβ: 15977
STING: 72512"
Figure legend: "(C) 293T cells were co-transfected with expression plasmids for Cherry-STING and indicated chimeras as shown in (A). An anti-V5 IP was performed and samples were analyzed by IB with indicated antibodies. IB shown is representative of three independent experiments."
NCBI gene IDs:
"Cherry: 
STING: 72512"
Figure legend: "(D) Cherry-STING, IFNβ-Luc, pRL-TK and either CD4, m152, m152-Δstalk or m152-GSstalk were transiently expressed in 293T cells. For stimulation, samples were co-transfected with cGAS-GFP, and unstimulated samples with IRES-GFP. Lysates were analyzed as described in (B). Data is combined from two out of three independent experiments and shown as mean ± SD. Data information: Student's t-test (unpaired, two-tailed), n.s. not significant, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001"
NCBI gene IDs:
"Cherry: 
GFP: 
Luc: 
m152: 
pRL: 
TK: 
CD4: 12504
cGAS: 115004
IFNβ: 15977
STING: 72512"
Figure legend: "(E) 293T cells were co-transfected with expression plasmids for Cherry-STING and either CD4, m152, m152-Δstalk or m152-GSstalk. An anti-V5 IP was performed and samples were analyzed by IB with indicated antibodies. Immunoblot shown is representative of three independent experiments."
NCBI gene IDs:
"Cherry: 
m152: 
CD4: 12504
STING: 72512"
Figure legend: "(A) 293T cells were co-transfected with expression plasmids for IFNβ-Luc, pRL-TK, cGAS-GFP (stimulated) or IRES-GFP (unstimulated) and indicated expression plasmids together with murine Cherry-STING (left panel) or human STING (right panel). Lysates were analyzed by dual-luciferase assay. Data is combined from two out of three independent experiments and shown as mean ± SD."
NCBI gene IDs:
"Cherry: 
GFP: 
Luc: 
pRL: 
TK: 
cGAS: 115004
IFNβ: 15977
STING: 72512
STING: 340061"
Figure legend: "(C) IFNβ-Luc, pRL-TK, cGAS-GFP (stimulated) or IRES-GFP (unstimulated) and either LacZ or m152 together with the indicated murine STING mutants described in (B) were transiently expressed in 293T cells. Luciferase activity was measured as described in (A). Data information: Data in (C) is normalized to LacZ, combined from three independent experiments and shown as mean ± SD."
NCBI gene IDs:
"GFP: 
LacZ: 
Luc: 
m152: 
pRL: 
TK: 
cGAS: 115004
IFNβ: 15977
STING: 72512"
Figure legend: "(D) 293T cells were co-transfected with expression plasmids for either LacZ or m152 together with the indicated murine STING mutants described in (B). An anti-V5 IP was performed and samples were analyzed by IB with the indicated antibodies. IB shown in (D) representative of three independent experiments."
NCBI gene IDs:
"LacZ: 
m152: 
V5: 
STING: 72512"
Figure legend: "(E-F) Luciferase assay (E) were performed as described in (C) and (D), respectively, using the indicated human STING mutants in place of the murine STING mutants described in (B). Data information: Data in (E) is normalized to LacZ, combined from three independent experiments and shown as mean ± SD."
NCBI gene IDs:
"LacZ: 
STING: 340061
STING: 72512"
Figure legend: "(E-F) co-IP (F) were performed as described in (C) and (D), respectively, using the indicated human STING mutants in place of the murine STING mutants described in (B). Data information: IB shown in (F) are representative of three independent experiments."
NCBI gene IDs:
"STING: 340061
STING: 72512"
Figure legend: "(B) 293T cells were co-transfected with expression plasmids for IFNβ-Luc, pRL-TK and either ev or m152. Cells were further co-transfected with either Cherry-STING WT or the constitutively active Cherry-STING V154M (stimulated) or with IRES-GFP (unstimulated). Data is combined from three independent experiments and shown as mean ± SD."
NCBI gene IDs:
"Cherry: 
GFP: 
Luc: 
m152: 
pRL: 
TK: 
IFNβ: 15977
STING: 72512"
Figure legend: "(C) iMEF stably expressing ev or m152-V5 were stimulated with 10 µg/ml ISD for the indicated times or left unstimulated (mock). Cell lysates were analyzed by SDS-PAGE under non-reducing conditions and subjected to IB with the specified antibodies. Data information: IB shown in (C) are representative of three and two independent experiments, respectively. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, ****p&lt;0.0001"
NCBI gene IDs:
"m152: 
V5: "
Figure legend: "(D) Two representative still images from live cell imaging experiments with iMEFgt/gt stably expressing Cherry-STING transfected with ISD (right panel) or left unstimulated (left panel). Red circles highlight representative translocated STING in ISD stimulated cells, which is used as an indicator of activation."
NCBI gene IDs:
"Cherry: 
STING: 72512"
Figure legend: "(E) iMEFgt/gt stably expressing Cherry-STING and either ev or m152-V5 were stimulated with ISD. Live cell imaging was performed and STING translocation quantified 120 min and 180 min post stimulation. Data shown is one representative of two independent experiments."
NCBI gene IDs:
"Cherry: 
m152: 
V5: 
STING: 72512"
Figure legend: "(F) iMEFgt/gt stably expressing Cherry-STING and V5-tagged m152 or corresponding ev were stimulated with 5 µg/ml ISD for the indicated time or left unstimulated (mock). Lysates were subjected to IB with the specified antibodies. Data information: IB shown in (C) and (F) are representative of three and two independent experiments, respectively. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, ****p&lt;0.0001"
NCBI gene IDs:
"Cherry: 
m152: 
V5: 
STING: 72512"
Figure legend: "(B) iMEF were infected by centrifugal enhancement with either parental MCMV (par.) or MCMV m152stop at an MOI of 0.5 or mock infected. 3 hours post infection (hpi) cells were lysed and subjected to immunoblotting (IB) with the specified antibodies."
NCBI gene IDs:
"m152: "
Figure legend: "(C) iMEF and iMEFgt/gt were infected by centrifugal enhancement with parental MCMV or MCMV m152stop, or parental MCMV alone, respectively (MOI 0.5). 3 hpi lysates were subjected to an anti-m152 IP and samples were analyzed by IB with indicated antibodies. IB shown is representative of two independent experiments."
NCBI gene IDs:
"m152: "
Figure legend: "(D) Primary BMDM were infected with parental MCMV (par.) or MCMV m152stop at an MOI of 0.1 or mock infected. 16 hpi secreted levels of IFNα or IFNβ were quantified by ELISA. Data is representative of two independent experiments."
NCBI gene IDs:
"m152: "
Figure legend: "(E) iMEFgt/gt stably expressing Cherry-STING were infected with parental MCMV or MCMV m152stop (MOI 0.5). Live cell imaging was performed and STING translocation quantified 120 min and 180 min post stimulation. Data shown is one representative of two independent experiments."
NCBI gene IDs:
"Cherry: 
m152: 
STING: 72512"
Figure legend: "(F-G) iMEF or iMEFgt/gt were infected by centrifugal enhancement with parental MCMV or MCMV m152stop (MOI 0.01). At 6 hpi total RNA was extracted to determine MCMV IE1 and MCMV E1 (F), transcripts by qRT-PCR. Data shown is combined from two out of three independent experiments."
NCBI gene IDs:
"E1: 
IE1: 
m152: "
Figure legend: "(F-G) iMEF or iMEFgt/gt were infected by centrifugal enhancement with parental MCMV or MCMV m152stop (MOI 0.01). At 6 hpi total RNA was extracted to determine , IFNb1 and IL6 (G) transcripts by qRT-PCR. Data shown is combined from two out of three independent experiments."
NCBI gene IDs:
"m152: 
IFNb1: 15977
IL6: 16193"
Figure legend: "(H) 293T cells were co-transfected with Cherry-STING, the pNF-κB luciferase reporter, pRL-TK, cGAS-GFP (stimulated) or IRES-GFP (unstimulated) and either ev or m152. Cells were lysed and analyzed as described in Figure 1. Data is combined from three independent experiments."
NCBI gene IDs:
"Cherry: 
GFP: 
luciferase: 
m152: 
pRL: 
TK: 
cGAS: 115004
pNF-κB: 18033
STING: 72512"
Figure legend: "(A-C) Type I IFN levels in spleen homogenates (A) and serum (B) of C57BL/6J (B6J) or STING-/- mice following i.v. infection with 4 x 105 PFU parental MCMV or MCMV m152stop were analyzed 6 hpi by ELISA. IFNβ levels of STING-/- mice were below the detection limit (n.d.)."
NCBI gene IDs:
"m152: 
STING: 72512"
Figure legend: "IL6 levels in spleen homogenates (C) of C57BL/6J (B6J) or STING-/- mice following i.v. infection with 4 x 105 PFU parental MCMV or MCMV m152stop were analyzed 6 hpi by ELISA."
NCBI gene IDs:
"m152: 
STING: 72512"
Figure legend: "(D) B6J or STING-/- mice were i.v. infected with 4 x 105 PFU parental MCMV or MCMV m152stop. 6 hpi, RNA was extracted from spleen homogenates and expression of MCMV IE1 and E1 transcripts was determined by qRT-PCR. Data were normalized to 107 cellular β-actin transcripts (D-F)."
NCBI gene IDs:
"E1: 
IE1: 
m152: 
β-actin: 
STING: 72512"
Figure legend: "(E) B6J or STING-/- mice were i.v. infected with 4 x 105 PFU parental MCMV. 6 hpi, RNA was extracted from spleen homogenates and m152 transcript levels were determined by qRT-PCR. Data were normalized to 107 cellular β-actin transcripts (D-F)."
NCBI gene IDs:
"m152: 
β-actin: 
STING: 72512"
Figure legend: "(F) B6J or STING-/- mice were depleted of NK cells via treatment with anti-NK1.1 (right panel) or left untreated (left panel). One day after NK cell depletion, B6J or STING-/- mice were i.v. infected with 4 x 105 PFU parental MCMV or MCMV m152stop. 16 hpi, RNA was extracted from spleen homogenates and MCMV E1 transcript levels were determined by qRT-PCR. Data were normalized to 107 cellular β-actin transcripts (D-F)."
NCBI gene IDs:
"E1: 
m152: 
β-actin: 
STING: 72512"
Figure legend: "(A) 293T cells were co-transfected with Cherry-STING, pRL-TK, cGAS-GFP (stimulated) or IRES-GFP (unstimulated) and either the pNF-κB, p55-CIB, p125 or p125AA luciferase reporter. Data is representative of two independent experiments."
NCBI gene IDs:
"Cherry: 
GFP: 
luciferase: 
pRL: 
TK: 
cGAS: 115004
STING: 72512"
Figure legend: "(B) 293T cells were co-transfected with expression plasmids for cGAS-GFP (stimulated) or ev (unstimulated) together with either cherry-tagged WT STING or cherry-tagged K288R STING. Cells were fixed for imaging 24 hours post transfection. Scale bar represents 10 µm."
NCBI gene IDs:
"cherry: 
GFP: 
cGAS: 115004
STING: 72512"
Figure legend: "(C-D) iMEFgt/gt stably expressing cherry-tagged WT STING or K288R STING and either ev or V5-tagged m152 were stimulated with ISD (10 µg/ml) or mock stimulated. At 4 hpi total RNA was extracted to determine IFNb1 (C) and IL6 (D) transcripts by qRT-PCR. Data shown is combined from three (C) or two (D) out of three independent experiments."
NCBI gene IDs:
"cherry: 
m152: 
V5: 
IFNb1: 15977
IL6: 16193
STING: 72512"
Figure legend: "(E) iMEFgt/gt (-) and iMEFgt/gt stably expressing either cherry-tagged WT STING or K288R STING were infected by centrifugal enhancement with parental MCMV or MCMV m152stop (MOI 0.01). 6 hpi total RNA was extracted to determine MCMV E1 transcripts by qRT-PCR. Data shown is combined from three independent experiments."
NCBI gene IDs:
"E1: 
cherry: 
m152: 
STING: 72512"
Figure legend: "b, Starved NRK cells expressing LAMP1-YFP show tubules (arrows). Box is expanded on the right."
NCBI gene IDs:
"LAMP1: 25328"
Figure legend: "d, LAMP1-YFP expressing NRK-LC3 cells as in a. Arrows show reformation tubules. e, Three-dimensional reconstruction of a cell from d."
NCBI gene IDs:
"LC3: 362245///64862"
Figure legend: "a, TEM (bottom; scale bar, 5 µm) and fluorescence (top; scale bar, 10 µm) of NRK-LC3 cells starved for times shown with enlargements (boxes)."
NCBI gene IDs:
"LC3: 362245///64862"
Figure legend: "b, d, Immunoblots of cells shown in a with indicated antibodies (d, after transfection with non-specific (NS) or atg5 RNAi). c, Autophagy (black lines  show  independent experiments) and phospho-S6K percentage (densitometry of data shown in b, red line)."
NCBI gene IDs:
"atg5: 365601"
Figure legend: "e-g, LAMP1-YFP NRK-LC3 cells starved for 2 h and then treated with 100 nM rapamycin for another 4 h (total, 6 h) or 6 h (total, 8 h) analysed by blotting (e) as in b or microscopy (f; scale bar, 5 µm) with quantification (g). Error bars, s.e.m.; n = 3."
NCBI gene IDs:
"LC3: 362245///64862"
Figure legend: "a, LAMP1/LC3NRK cells were starved for 4 h and treated with GTPγS for 6 h of further starvation."
NCBI gene IDs:
"LC3: 362245///64862"
Figure legend: "b, Rab7Q67L-GFP-transfected LC3-CFP/Lamp1-RFP NRK cells starved for 10 h."
NCBI gene IDs:
"Lamp1: 25328
LC3: 362245///64862
Rab7: 501854///29448"
Figure legend: "c, Rab7-CFP NRK cells starved for 2 h and treated with 100 nM rapamycin for 8 h of further starvation."
NCBI gene IDs:
"Rab7: 501854///29448"
Figure legend: "d, LAMP1-YFP NRK-LC3 cells starved for times shown with 1 µg ml-1 leupeptin and blotted with indicated antibodies. e, Cells in d imaged.."
NCBI gene IDs:
"LC3: 362245///64862"
Figure legend: "(B) HCT116 cells in which eIF3k was homozygously modified with the mAID domain to trigger auxin-dependent degradation were exposed to 500 μM indole-3-acetic acid (IAA) for the periods shown, and cell lysate was subjected to immunoblotting with the indicated antibodies"
NCBI gene IDs:
"eIF3k: 27335"
Figure legend: "(A) eIF3k-mAID cells were maintained in media containing different concentrations of FBS and DMSO or IAA as indicated, and cell numbers were determined at various time points. Data represent means ± SD, n = 3 biological replicates. Numbers indicate p values"
NCBI gene IDs:
"eIF3k: 27335"
Figure legend: "(B) eIF3k-mAID cells were serum starved by maintaining in media containing 0% FBS for 24 h. During the last 12 h of starvation, DMSO or IAA was added as indicated. Cells were re-stimulated with media containing 10% FBS, and the expression of the indicated proteins was followed of a period of 120 minutes. The data from triplicate experiments were quantified and plotted as relative ratios of phosphorylated to unphosphorylated species. Bars represent means ± SD, n = 3 Numbers indicate p values (unpaired Student's t-test)."
NCBI gene IDs:
"eIF3k: 27335"
Figure legend: "(C) eIF3k-mAID cells were exposed to IAA or DMSO for 12 hours, followed by the addition of increasing concentrations of rapamycin for 1 h. The expression of the indicated proteins was determined by immunoblotting. The data from triplicate experiments were quantified and plotted as relative ratios of phosphorylated to unphosphorylated species. Bars represent means ± SD, n = 3 Numbers indicate p values (unpaired Student's t-test)."
NCBI gene IDs:
"eIF3k: 27335"
Figure legend: "(C) Total ribosome occupancy of the indicated mRNAs in eIF3k-mAID cells exposed to DMSO or IAA for 12 hours was determined by RT-qPCR of RNA across a sucrose density gradient Bars represent means ± SD, n = 3; numbers indicate p values (unpaired Student's t-test). Triplicate RT-qPCR data across the sucrose gradient are shown below the bar graphs."
NCBI gene IDs:
"eIF3k: 27335"
Figure legend: "(D) Equal numbers of eIF3k-mAID cells stably expressing ectopic RPS15A (pCDH-RPS15A) or empty vector (pCDH) were plated and counted over a period of 6 days. Graphs represent means ± SD, n = 3. Numbers indicate p values"
NCBI gene IDs:
"eIF3k: 27335
RPS15A: 6210"
Figure legend: "(G) Equal numbers of eIF3k-mAID cells stably expressing ectopic RPS4X (pCDH-RPS4X) or empty vector (pCDH) were plated and counted over a period of 6 days. Graphs represent means ± SD, n = 3. Numbers indicate p values Total ribosome content of these cells was determined as described in (A)."
NCBI gene IDs:
"eIF3k: 27335
RPS4X: 6191"
Figure legend: "(B) Parental eIF3k-mAID cells or S15A-eIF3KO cells (clones #45 and # 361) were maintained in standard media, and cell numbers were determined at various time points. Data represent means ± SD, n = 3. Asterisks denote: * p &lt; 0.005, ** p &lt; 0.00005, *** p &lt; 0.000005"
NCBI gene IDs:
"eIF3k: 27335
S15A: 6210"
Figure legend: "(D) Relative translational efficiencies (TEs) of mRNAs encoding RPS15A, RPS4X, and RPL7A before and after depletion of eIF3k were determined in the indicated cell lines. RT-qPCR was performed on total RNA and on RNA contained within polysomal fractions &gt; 2 ribosomes, and TE was calculated according to the formula TE = polysomal mRNA / total mRNA. Bars represent means ± SD, n = 3; numbers indicate p values (unpaired Student's t-test)."
NCBI gene IDs:
"eIF3k: 27335
RPL7A: 6130
RPS15A: 6210
RPS4X: 6191"
Figure legend: "(A) Parental eIF3k-mAID cells or S15A-eIF3KO cells (clones #45 and # 361) were maintained in media with DMSO or IAA, and cell numbers were determined at various time points. Data represent means ± SD (too small to be visible), n = 3. All p values were &gt; 0.45 except at the single time point where indicated otherwise."
NCBI gene IDs:
"eIF3k: 27335
S15A: 6210"
Figure legend: "(D) Total ribosome occupancy of the indicated mRNAs in S15A-eIF3KO (clone #361) cells exposed to DMSO or IAA for 12 hours was determined by RT-qPCR of RNA across a sucrose density gradient Bars represent means ± SD, n = 3; numbers indicate p values (unpaired Student's t-test). Triplicate RT-qPCR data across the sucrose gradient are shown below the bar graphs."
NCBI gene IDs:
"S15A: 6210"
Figure legend: "(E) RNA immunoprecipitation. eIF3k-mAID and S15A-eIF3KO (clone #361) cells were exposed to DMSO or IAA for 12 hours. Cell lysates were employed in immunoprecipitation with eIF3c antibodies and co-precipitated mRNAs were quantified by qPCR. Bars represent means ± SD, n = 4; numbers indicate p values (unpaired Student's t-test)."
NCBI gene IDs:
"eIF3k: 27335
S15A: 6210"
Figure legend: "(A) Comparative permissiveness of various organs from B6.K18-hACE2IP-THV and B6.K18-ACE22prlmn/Jax transgenic mice (n = 5-6/group) to SARS-CoV-2 replication, as determined at 3 dpi by conventional E-specific or sub-genomic Esg-specific  qRT-PCR. Red lines indicate the  qRT-PCR  limits of detection. (B) Comparative quantitation of  hACE-2 mRNA in the  lungs  and  brain of B6.K18-hACE2IP-THV and B6.K18-ACE22prlmn/Jax transgenic mice (n = 5-6/group). Data information: Statistical significance was evaluated by Mann-Whitney test (*= p &lt; 0.05, **= p &lt;0.01, ns = not significant)."
NCBI gene IDs:
"hACE-2: 59272
ACE2: 59272
hACE2: 59272
Esg: 43740570
K18: 100775105"
Figure legend: "(C) Heatmaps represent log2 fold change in cytokine and chemokine mRNA expression in the lungs or brain  of B6.K18-hACE2IP-THV and B6.K18-ACE22prlmn/Jax mice at 3 dpi (n = 5-6/group). Data were normalized versus untreated controls."
NCBI gene IDs:
"ACE2: 59272
hACE2: 59272
K18: 100775105"
Figure legend: "(C)  Viral RNA  content as determined in various organs at 3 dpi (n = 6/group) by use of conventional E-specific or sub-genomic  Esg-specific  qRT-PCR. Red lines indicate the qRT-PCR detection limits. Data information: Statistical significance was evaluated by Mann-Whitney test (* = p&lt;0.05, ** = p&lt;0.01)."
NCBI gene IDs:
"Esg: 43740570"
Figure legend: "(D) Percentages of NK cells or neutrophils in the lungs  of LV::S- or sham-vaccinated and SARS-CoV-2-challenged B6.K18-hACE2IP-THV transgenic mice  at 3 dpi (n = 6/group). Percentages were calculated versus total lung live  CD45+ cells. Data information: Statistical significance was evaluated by Mann-Whitney test (* = p&lt;0.05, ** = p&lt;0.01)."
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "(E) Relative log2 fold change in cytokine and chemokine mRNA expression in the  brain of LV::S- or sham-immunized and SARS-CoV-2-challenged B6.K18-hACE2IP-THV transgenic mice at 3 dpi (n = 6/group). Means ± SD are shown. Data were normalized versus untreated controls. Data information: Statistical significance was evaluated by Mann-Whitney test (* = p&lt;0.05, ** = p&lt;0.01). "
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "B6.K18-hACE2IP-THV mice were primed (i.m.) at wk 0 and boosted (i.n.) at wk 5 (n = 5) with non-integrative LV::S. Control mice were injected with an empty LV (sham). (B, C) Cytometric strategy to detect  lung CD8+ T central memory (Tcm, CD44+CD62L+CD69-), T effector memory  (Tem, CD44+CD62L-CD69-) and T resident memory (Trm, CD44+CD62L-CD69+CD103+) and (C) percentages of these subsets among CD8+ T-cells in LV::S-vaccinated (n = 9) or sham (n = 5)  mice. Data information: Statistical significance of the difference between the two groups was evaluated by Mann-Whitney test (*= p &lt; 0.05, **= p &lt;0.01)."
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "B6.K18-hACE2IP-THV mice  were primed (i.m.) at wk 0 and boosted (i.n.) at wk 5 (n = 5) with non-integrative LV::S. Control  mice were injected with an empty LV (sham). (D)  Cytometric strategy to detect  SCoV-2-specific CD8+ T  cells by use of the H-2Db-SCoV-2:538-546 dextramer in the lungs of LV::S or sham-vaccinated mice. Inside  CD8+ dextramer+ T-cell subset, Tcm, Tem and Trm have been distinguished."
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "B6.K18-hACE2IP-THV mice were primed (i.m.) at wk 0 and boosted (i.n.) at wk 5 (n = 5) with non-integrative LV::S. Control mice were injected with an empty LV (sham). (F, G) Anti-SCoV-2 IgG or  IgA  titers (F) and neutralizing activity (EC50) (G) in the  sera or lung homogenates at 3 dpi. Samples from individual mice (n = 4/group) were studied. Data information: Statistical significance of the difference between the two groups was evaluated by Mann-Whitney test (*= p &lt; 0.05, **= p &lt;0.01). "
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "(A) B6.K18-hACE2IP-THV mice were immunized with LV::S via i.m. or i.n. at wk 0 and boosted via i.m. or i.n. at wk 5 (n = 5/group) with non-integrative LV::S. Control mice were injected i.m. i.n. with an empty LV (sham). Viral RNA  contents were determined by conventional E-specific RT-PCR at 3 dpi, in the brain, lung and nasal washes. Data information: Statistical significance was evaluated by Mann-Whitney test (*= p &lt; 0.05, **= p &lt;0.01, ****= p &lt; 0.0001, ns = not significant)."
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"
Figure legend: "(B) Lung histology in B6.K18-hACE2IP-THV mice, vaccinated with LV::S after SARS-CoV-2 inoculation. H&amp;E (rows 1 and 3) (scale bar: 500 μm) and NCoV-2-specific  IHC (rows 2 and 4) (scale bar: 50 μm)  staining of whole lung sections (scale bar: 50 μm) from the primed (i.m.), boosted (i.n.) and challenged B6.K18-hACE2IP-THV mice compared with their sham controls. H&amp;E and NCoV-2-specific  IHC were performed on contiguous sections. The IHC fields correspond to the rectangles in the corresponding H&amp;E images above them. A representative NCoV-2-specific IHC on a lung section from a non-infected (NI) mouse is also shown."
NCBI gene IDs:
"hACE2: 59272
K18: 100775105"