EMBO/SourceData huggingface dataset:
Figure legend: " (A) Depletion of KLF4 significantly enhances the efficacy of Olaparib in killing TNBC cells (4T1). 4T1 cells with stable expression of shKLF4 were implanted into mammary fat pad of BALB/c Nude mice. Drug treatment was started at 10th day. Placebo or Olaparib at the dose of 100 mg/kg was administrated daily for twelve days. Tumor volumes were measured every other day. Tumor volumes were measured every other day. n=9 per group, p values were labeled in figure, one-way ANOVA assay. "
NCBI gene IDs:
"KLF4: 16600"
Figure legend: "C, Dusp1 mRNA levels on P74. Two-tailed unpaired t test. t = 0.3691, p = 0.7307. n = 3 biological replicates per group. Data information: ASE, adolescent saline-exposed mice during adulthood; ACE, adolescent cocaine-exposed mice during adulthood The data were presented as the mean ± SEM. Statistical analyses were performed using unpaired t-tests. N.S., p &gt; 0.05, *, p &lt; 0.05, **, p &lt; 0.01 versus ASE mice"
NCBI gene IDs:
"Dusp1: 19252"
Figure legend: "B, Dusp1 mRNA levels on P78. Two-tailed unpaired t test. t = 4.413, p = 0.0116. n = 3 biological replicates per group. Data information: Ctrl, control virus-treated mice; OE, overexpression virus-treated mice. The data were presented as the mean ± SEM. Statistical analyses were performed using Two-way ANOVA or unpaired t-tests. N.S., p &gt; 0.05, #, p &lt; 0.05, versus baseline (pre-test CPP score); N.S., p &gt; 0.05, *, p &lt; 0.05, **, p &lt; 0.01 versus Ctrl virus-treated mice."
NCBI gene IDs:
"Dusp1: 19252"
Figure legend: "B, Dusp1 mRNA levels on P78. Two-tailed unpaired t test. t = 9.250, p &lt; 0.0001. n = 4 biological replicates per group. Data information: Ctrl, control virus-treated mice; KD, knocking-down virus-treated mice. The data were presented as the mean ± SEM. Statistical analyses were performed using Two-way ANOVA or unpaired t-tests. N.S., p > 0.05, ##, p &lt; 0.01 versus baseline (pre-test CPP score); N.S., p > 0.05, *, p &lt; 0.05, **, p &lt; 0.01 versus Ctrl virus-treated mice."
NCBI gene IDs:
"Dusp1: 19252"
Figure legend: "(C) Cultured neurons and (D) DRG section from Avil-Cre::Ret+/eGFPmice displaying native eGFPfluorescence and stained with IB4. Different levels of eGFP intensity (high -indicated by arrows and low -indicated by arrowheads) are detected across IB4+ and IB4- cells. Scale bar, 50 μm."
NCBI gene IDs:
"Cre: "
Figure legend: "(A) Ret-eGFPHi:IB4Neg and Ret-eGFPLo:IB4Negneurons display distinct expression profiles. A volcano plot of fold change expression in Ret-eGFPLo:IB4Neg versus Ret-eGFPHi:IB4Neg against probability. Ret has higher expression in the Ret-eGFPHi:IB4Neg subset, which also shows an up regulation of the Ret co-receptor Gfra2 and Fam38b, encoding for the mechanosensitive ion channel Piezo2. The Ret-eGFPLo:IB4Neg subset, displays an array of molecules previously associated with itch (marked in red)."
NCBI gene IDs:
"Gfra2: 14586
Fam38b: 667742
Piezo2: 667742"
Figure legend: "(B) Itch-associated transcripts are enriched in the Ret-eGFPLo:IB4Neg population compared to all DRGneurons. Volcano plot of fold change expression in Ret-eGFPLo:IB4Negneurons versus sorted neurons from Avil-Cre::R26tdRFPmice against probability. Molecules linked to itch perception are significantly upregulated in the Ret-eGFPLo:IB4Neg subset."
NCBI gene IDs:
"Cre: 
R26: 14910
Ret: 19713"
Figure legend: "(C) Gene expression within the Ret-eGFPLo:IB4Neg population is confirmed by triple comparison between Ret-eGFPLo:IB4Neg, Ret-eGFPHi:IB4Neg and Avil-Cre::R26tdRFPdatasets."
NCBI gene IDs:
"Cre: 
R26: 14910"
Figure legend: "(A−H) SstCre mediated recombination of the Ret locus drives eGFP expression in sensory neurons that do not bind to IB4 or co-express NF200 or TH. Triple immunostaining of DRG from Sst-Cre::ReteGFP/+mice with RetGFP (A), IB4 (B) and NF200 (C), and RetGFP (E), IB4 (F) and TH (G)."
NCBI gene IDs:
"Cre: 
Ret: 19713
Sst: 20604"
Figure legend: "(I) Quantification of SstCre::Ret-eGFP expression in DRG. (J) Quantification of co-expression of SstCre::Ret-eGFP with neuronal markers (n=8827 cells from 3 mice)."
NCBI gene IDs:
"Sst: 20604"
Figure legend: "(K−M) Peripheral projections of sensory neurons from Sst-Cre::Rosa26SnapCaaXmice terminate in the hairy skin as free nerve endings. Non-innervated hair follicles are indicated by arrows. Double labelling using TMRStar to label SNAP-tag (K) and DAPI (L). Scale bars, 50 μm."
NCBI gene IDs:
"Cre: 
Rosa26: 14910
Sst: 20604"
Figure legend: "(A-B) Contour diagram of dissociated sensory neurons from Sst-Cre::ReteGFP/+ (A) and Avil-Cre::ReteGFP/+neurons (B), plotted according to their native eGFP expression and IB4 binding. Only one subset of Ret-eGFP cells can be defined within the Sst-Cre::ReteGFP/+ dissociated DRGneurons. This displays the same range of eGFPfluorescence as the Ret-eGFPLo:IB4Neg population and doesn't bind to IB4."
NCBI gene IDs:
"Cre: 
Sst: 20604"
Figure legend: "(C) Quantitative RT-PCR analysis of selected transcripts in the Sst-Cre::ReteGFP (darker colors) and Ret-eGFPHi:IB4Neg (lighter colors) subsets displayed as differential expression compared to the Ret-eGFPHi:IB4Neg subset. Almost all the genes that are up-regulated in the Ret-eGFPLo:IB4Neg compared to the Ret-eGFPHi:IB4Neg subset are also enriched in the Sst-Cre::ReteGFP population (n=3)"
NCBI gene IDs:
"Cre: 
Sst: 20604"
Figure legend: "(A-G) Ablation of Sst-Cre::AviliDTR positive sensory neurons by injection of diphtheria toxin (DTX). Immunostaining of DRG from Sst-Cre::AviliDTRmice with an antibody against the diphtheria toxin receptor and IB4 in the absence of diphtheria toxin (A-C) and after systemic injection of diphtheria toxin (D-F)."
NCBI gene IDs:
"Sst: 20604"
Figure legend: "(A-B) Nociceptive withdrawal thresholds in control (AviliDTR without the Cre) and Sst-Cre::AviliDTRmice after systemic diphtheria toxin injection. No significant differences in thermal (A) or mechanical (B) withdrawal reflexes after ablation of SstCre positive neurons."
NCBI gene IDs:
"Sst: 20604"
Figure legend: "(C-F) Scratching behavior after a single intradermal injection of the indicated pruritogen observed for 1 hour. All compounds evoked a significant increase in the number of scratching bouts compared to vehicle alone in control (AviliDTR) or Sst-Cre::AviliDTRmice. Diphtheria toxin injection in control mice (AviliDTR) had no effect on scratching behavior evoked by histamine (C), chloroquine (D), IL-31 (E) or 5HT1F agonist Ly344864 (F) but significantly reduced responses to IL31, or Ly344864 in Sst-Cre::AviliDTRmice. n-numbers indicated in brackets, asterisk denotes p&lt;0.05, two way RM ANOVA, Holm-Sidak multiple comparison. Error bars indicate SEM."
NCBI gene IDs:
"Sst: 20604"
Figure legend: "qRT-PCR gene expression analysis showing the fold change of Pi3K/mTOR associated gene panel in KSL cells sorted from: (A) Molm-14-xenografted mice"
NCBI gene IDs:
"mTOR: 56717
Pi3K: 18706"
Figure legend: "qRT-PCR gene expression analysis showing the fold change of Pi3K/mTOR associated gene panel in KSL cells sorted from: IF injected mice with Molm-14-EV relative to their controls and normalized to Gapdh endogenous control."
NCBI gene IDs:
"Gapdh: 
mTOR: 56717
Pi3K: 18706"
Figure legend: "Dual Luciferase reporter assay. NIH-3T3 was transfected with the miRNA mimics. Three hours later the cells were transfected with the Raptor 3′UTR cloned into the psiCheck-2 vector for a total of 48 hours. Data are presented as %RLU (relative luciferase units) of the miR-scramble control as means ± SEM from at least three independent experiments, performed in technical replicates."
NCBI gene IDs:
"Raptor: 74370"
Figure legend: "Cell-cycle flow cytometric analysis using Ki67/Hoechst-33342 staining of the percentage of LT-HSC in the G0 phase after nucleofection of cKit+ cells using the AmaxaTM P3 Primary Cell 4D-Nucleoeftcor kit, (J) Cells were nucleofected with miR-Scramble (CTRL) or miR-1246 mimic (n=3) for 72 hours,"
NCBI gene IDs:
"miR-1246: "
Figure legend: "Cell-cycle flow cytometric analysis using Ki67/Hoechst-33342 staining of the percentage of LT-HSC in the G0 phase after nucleofection of cKit+ cells using the AmaxaTM P3 Primary Cell 4D-Nucleoeftcor kit, Cells were nucleofected with Anti-miR-Scramble (CTRL) or Anti-miR-1246 (n=5) and 1-hour later co-treatment with Molm-14 EVs for 72 hours."
NCBI gene IDs:
"miR-1246: "
Figure legend: "Evaluation of the in vivo long-term repopulation capacity. For secondary transplantation (n=6 per group), PB samples were collected twice weekly for 12 weeks (WK) and the donor chimerism was measured by qPCR of Sex-determining Region Y (SRY) normalized to Gapdh gatekeeper as shown in the left panel. Sixteen weeks later, mice were sacrificed and BM male chimerism percentage was assessed as shown in the right panel. Tertiary transplantation, unfractionated BM cells from the secondary recipients (n=6 per group, 106 cells per mouse) were injected into tertiary 150cGy sub-lethally irradiated female recipients and blood SRY levels were assessed for 20 weeks."
NCBI gene IDs:
"Gapdh: 
SRY: 6736
Sex-determining Region Y: 6736"
Figure legend: "qRT-PCR gene expression analysis showing the fold-change of select DNA-damage response-genes panel in Molm-14-xenograft-derived: (E) Primary recipient KSL, n=6 Data indicated by bars of fold-change calculated relative to matching controls, normalized to the endogenous control Gapdh and presented as means ± SEM."
NCBI gene IDs:
"Gapdh: "
Figure legend: "qRT-PCR gene expression analysis showing the fold-change of select DNA-damage response-genes panel in Molm-14-xenograft-derived: Cells from W1 plating, n=4, Data indicated by bars of fold-change calculated relative to matching controls, normalized to the endogenous control Gapdh and presented as means ± SEM."
NCBI gene IDs:
"Gapdh: "
Figure legend: "qRT-PCR gene expression analysis showing the fold-change of select DNA-damage response-genes panel in Molm-14-xenograft-derived: Cells from W2 plating n=4. Data indicated by bars of fold-change calculated relative to matching controls, normalized to the endogenous control Gapdh and presented as means ± SEM."
NCBI gene IDs:
"Gapdh: "
Figure legend: "qRT-PCR analysis of the DNA-damage-genes panel in Molm-14-xenograft-derived: (M) KSL, and (N) LT-HSC, relative to matched controls. Data depicted as fold-change relative to matching controls, normalized to the endogenous control Gapdh and presented as mean ± SEM."
NCBI gene IDs:
"Gapdh: "
Figure legend: "(C) Immunohistochemistry for C. burnetii in the lung on d7 after i.t. infection. Representative images and quantitation of lung tissue positive for C. burnetii (n=12-13 mice, pooled from three experiments, each normalized to the mean of WT or Acod1+/- infected mice). Each dot represents one mouse."
NCBI gene IDs:
"Acod1: 16365"
Figure legend: "representative images of liver sections and quantification of immunohistochemistry for C. burnetii in liver sections (G). n=6-11 mice (for NMII infections), pooled from two experiments. Each dot represents one mouse. (Mann-Whitney test comparing treatment and control group of NMII infected Acod1-/- mice in specified organs, *"
NCBI gene IDs:
"Acod1: 16365"
Figure legend: "D. qRT-PCR  analysis of PCIF1  mRNA in the indicated cell lines. Levels were normalized to GAPDH  expression. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"GAPDH: 2597
PCIF1: 63935"
Figure legend: "A. Western blot analysis of PCIF1  in HT29  and HCT116 cells depleted of PCIF1  using four different sgRNAs. GAPDH  served as a loading control."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "Matrigel invasion  assay  (B), of the indicated control and PCIF1-depleted CRC  cell lines. Representative images and quantification of cells are shown at the left and right, respectively, of each panel. Data are the mean ± SD of n=3 replicates/condition. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "fibronectin adhesion  assay (C), of the indicated control and PCIF1-depleted CRC  cell lines. Representative images and quantification of cells are shown at the left and right, respectively, of each panel. Data are the mean ± SD of n=3 replicates/condition. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "colony formation assay (D) of the indicated control and PCIF1-depleted CRC cell lines. Representative images and quantification of cells are shown at the left and right, respectively, of each panel. Data are the mean ± SD of n=3 replicates/condition. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "wound healing migration assay  (E) of the indicated control and PCIF1-depleted CRC  cell lines. Representative images and quantification of cells are shown at the left and right, respectively, of each panel. Data are the mean ± SD of n=3 replicates/condition. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "A. LC-MS/MS  identification and quantification of m6Am  or m6A  levels in mRNA from control and PCIF1-depleted HCT116  cells. Data are presented as the enrichment of methylated  vs unmethylated mRNA. Data are the mean ± SD of n=3 biological replicates. **P &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "B. Metagene plots analysis of   m6Am enrichment around the transcription start site (TSS) of all expressed genes in control and PCIF1-depleted HCT116  cells."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "E. Western blot  analysis of FOS  and PCIF1  in control and PCIF1-depleted HCT116  and HT29  cells. GAPDH  served as a loading control."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "F. Genome browser views of FOS   with m6Am  sites. Read coverage of input sample and IP  sample are shown in blue and red, respectively, and green rectangle indicates the m6Am  peaks located near the TSS."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "G.  m6Am-exo-qPCR  analysis of m6Am enrichment in FOS  mRNAs from the indicated HCT116  cells. GAPDH  served as a negative control. Data are the mean ± SD of n=3 biological replicates. **P &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"FOS: 2353
GAPDH: 2597"
Figure legend: "H. qRT-PCR analysis of FOS  mRNA stability in control and PCIF1-depleted HCT116  and HT29  cells treated with actinomycin D for the indicated times. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"FOS: 2353
PCIF1: 63935"
Figure legend: "A. Western blot  analysis of FOS  in control and FOS-depleted HCT116  and HT29  cells. GAPDH  served as a loading control."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "MTS cell proliferation assay (B), of control and FOS-depleted HCT116   cells. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "wound healing migration assay (C), of control and FOS-depleted HCT116  cells. Representative images and quantification of colonies are shown to the left and right, respectively. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "colony formation assay (D) of control and FOS-depleted HCT116  cells. Representative images and quantification of colonies are shown to the left and right, respectively. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "E. Growth  of control and FOS-depleted HCT116  tumors after subcutaneous injection into athymic nude mice. Tumor volume was recorded on the indicated days. Data are the mean ± SEM of n=10 mice/group. **P &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "F. Survival  analysis of mice  bearing control and FOS-depleted HCT116  tumors. Data are the mean ± SEM of n= 5 mice/group. *P &lt; 0.05 by Student's t-test."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "B. mRNA expression of PCIF1  was quantified by qRT-PCR in the HCT116  cells transfected with gradient amount of control and PCIF1  siRNAs. Data are the mean ± SD of n=3 biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "C.  Western blot analysis of PCIF1  in HCT116  cells with control and PCIF1  siRNAs. GAPDH  served as a loading control."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "D. Cell viability assays were performed in in the HCT116  cells transfected with 100pmol control and PCIF1 siRNAs. Data are the mean ± SD of n=3 biological replicates. ***P &lt; 0.001 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "F. Tumor growth  of HCT116  cells treated with 1mg/kg control and PCIF1 LNP-siRNAs starting on day 9 and mice  were intratumorally treated twice a week. Data are the mean ± SEM of n=5 mice/group. *P &lt; 0.05, **P &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"PCIF1: 63935"
Figure legend: "A. Confocal images show Iba1-immunoreactive microglia and macrophages in RPE/choroid whole mount preparations 48 hours after laser coagulation and intravitreal application of low (0.2 µg) and high (3 µg) dose polySia avDP20 or PBS-vehicle. Laser spots of vehicle-injected SIGLEC11 transgenic (tg) and wild type control animals revealed strong accumulation of activated microglia and macrophages, which was effectively decreased in polySia avDP20-injected SIGLEC11 tg animals and to a lower extent in wild type controls. Representative images out of at least three independent experiments are shown. Scale bar: 50µm.B. Quantification of the average pixel intensity of Iba1-positive area in laser spots reflects accumulation of reactive microglia/macrophages on RPE/choroid whole mounts. In comparison to the accumulation of reactive microglia/macrophages in control animals, there is a reduced pixel intensity found in polySia avDP20-treated SIGLEC11 tg animals. Notably, treatment with a high polySia avDP20 dose (3 µg) also reduced accumulation of microglia/macrophages in laser spots of wild type animals. Data show mean +/- SD. * p&lt; 0.05, ** p&lt; 0.01, *** p&lt; 0.001, One-way ANOVA followed by Fisher's LSD. WT PBS (n = 24 spots), WT 0.2 µg Sia (n = 37 spots), WT 3 µg Sia (n = 20 spots), SIGLEC11 tg PBS (n = 25 spots), SIGLEC11 tg 0.2 µg Sia (n = 35 spots), SIGLEC11 tg 3 µg Sia (n = 31 spots), WT PBS vs WT 3 µg Sia p = 0.0147, WT 0.2 µg Sia vs SIGLEC11 tg 0.2 µg Sia p = 0.0046, SIGLEC11 tg PBS vs SIGLEC11 tg 0.2 µg Sia p = 0.0071, SIGLEC11 tg PBS vs SIGLEC11 tg 3 µg Sia p &lt; 0.0001."
NCBI gene IDs:
"SIGLEC11: "
Figure legend: "C. Confocal images of retinal whole mounts show Iba1-immunoreactive microglial cells. Retinal microglia of polySia avDP20-treated SIGLEC11 tg mice had a more ramified microglial morphology in the laser spot compared to PBS-vehicle treated mice. Interestingly, high polySia avDP20 dose (3 µg) also exerted a weak therapeutic effect in wild type animals. Representative images out of at least three independent experiments are shown. Scale bar: 20 µm.D. Percentage of retina showing activated microglial cells within the laser spots was quantified. PolySia avDP20 reduced the percentage of laser spots with activated microglia in SIGLEC11 tg animals and at high dose (3 µg) also in wild type controls. Data show mean +/- SEM. * p&lt; 0.05, ** p&lt; 0.01, ***p&lt;0.001, One-way ANOVA followed by Fisher's LSD. WT PBS (n = 6 retinas), WT 0.2 µg Sia (n = 8 retinas), WT 3 µg Sia (n = 6 retinas), SIGLEC11 tg PBS (n = 8 retinas), SIGLEC11 tg 0.2 µg Sia (n = 7 retinas), SIGLEC11 tg 3 µg Sia (n = 6 retinas),WT PBS vs WT 3 µg Sia p = 0.0052, WT 0.2 µg Sia vs SIGLEC11 tg 0.2 µg Sia p = 0.0005, SIGLEC11 tg PBS vs SIGLEC11 tg 0.2 µg Sia p = 0.0159, SIGLEC11 tg PBS vs SIGLEC11 tg 3 µg Sia p = 0.001."
NCBI gene IDs:
"SIGLEC11: "
Figure legend: "A. Fundus fluorescein angiography was performed 48 hours after laser coagulation and intravitreal application of low (0.2 µg) and high (3 µg) dose polySia avDP20 or PBS-vehicle. Late stage (10-11 minutes after fluorescein injection) fundus fluorescein angiography revealed that PBS-treated wild type controls and humanized SIGLEC11 mice showed normal levels of vessel leakage whereas polySia avDP20-treated SIGLEC11 transgenic (tg) mice had lower levels of vessel leakage compared to PBS-injected wild type or SIGLEC11 tg mice. High polySia avDP20 dose reduced vascular leakage also in wild type animals. Representative images out of at least eight independent experiments are shown.B. Fundus fluorescein angiography pictures were exported from Heidelberg Eye Explorer Software and fluorescein leakage was quantified with ImageJ software (NIH). Pixel intensities of 6 regions of interest per picture were quantified and background fluorescence was subtracted. PolySia avDP20-treated SIGLEC11 tg animals showed reduced vascular leakage compared to PBS-injected wild type control and SIGLEC11 tg mice. A reduction in vascular leakage was also observed in high dose polySia avDP20-injected wild type control mice. Data show mean +/- SD. * p&lt; 0.05, *** p&lt; 0.001, One-way ANOVA followed by Fisher's LSD. WT PBS (n = 20 eyes), WT 0.2 µg Sia (n = 17 eyes), WT 3 µg Sia (n = 9 eyes), SIGLEC11 tg PBS (n = 16 eyes), SIGLEC11 tg 0.2 µg Sia (n = 20 eyes), SIGLEC11 tg 3 µg Sia (n = 15 eyes), WT PBS vs WT 3 µg Sia p = 0.0193, WT 0.2 µg Sia vs SIGLEC11 tg 0.2 µg Sia p &lt; 0.0001, SIGLEC11 tg PBS vs SIGLEC11 tg 0.2 µg Sia p &lt; 0.0001, SIGLEC11 tg PBS vs SIGLEC11 tg 3 µg Sia p = 0.0002."
NCBI gene IDs:
"SIGLEC11: "
Figure legend: "C. Anti-C5b-9 immunostaining of RPE/choroid whole mount preparations 48 hours after laser damage showed strong MAC deposition in the laser lesions of vehicle-injected controls. PolySia avDP20 treatment reduced MAC formation in a dose-dependent fashion independent of SIGLEC11 presence. Scale bar: 100 µm.D. Quantification of C5b-9 fluorescence signal intensity in the laser lesions. In comparison to the high amount of MAC deposition in vehicle-injected controls, the pixel intensity is reduced in polySia avDP20-treated eyes in a dose-dependent fashion and regardless of SIGLEC11 presence. Data show mean +/- SD. *** p&lt; 0.001, One-way ANOVA followed by Fisher's LSD. WT PBS (n = 12 laser spots), WT 0.2 µg Sia (n = 12 laser spots), WT 3 µg Sia (n = 7 laser spots), SIGLEC11 tg PBS (n = 6 laser spots), SIGLEC11 tg 0.2 µg Sia (n = 10 laser spots), SIGLEC11 tg 3 µg Sia (n = 12 laser spots); all statistical comparisons p &lt; 0.0001 except SIGLEC11 tg PBS versus SIGLEC11 tg 0.2 µg Sia p = 0.0004."
NCBI gene IDs:
"SIGLEC11: "
Figure legend: "A. Analysis of relative TNFSF2 gene transcription in human control and SIGLEC11/16 knockout THP1-macrophages. The levels of gene transcripts were reduced after 24 hours of co-treatment with LPS (1 µg/ml) and concentrations of 0.15 µM and 1.5 µM of polySia avDP20 in the human wild type macrophages. No response to polySia avDP20 was detectable in the knockout line. Data show mean +/- SEM. ** p &lt; 0.01, ANOVA followed by Bonferroni. Statistical analysis was done in relation to the LPS control. WT: no treatment n=7 and p&lt; 0.0001, PolySia avDP20 1.5 µM n=4 and p=0.0002, LPS n=7, LPS/PolySia avDP20 0.15µM n=3 and p=0.009, LPS/PolySia avDP20 1.5µM n=5 and p=0.002. Siglec11/16 KO: no treatment n=5 and p=0.01, PolySia avDP20 n=4 and p=0.122, LPS n=7, LPS/PolySia avDP20 0.15µM n=3 and p=1.0, LPS/PolySia avDP20 1.5µM n=4 and p=1.0."
NCBI gene IDs:
"SIGLEC11: 114132
TNFSF2: 7124"