EMBO/SourceData huggingface dataset:
Figure legend: "D. Effect of LITATS1 knockdown on TGF-β-induced SMAD2 phosphorylation in MDA-MB-231 cells. Cells were serum starved for 16 h and stimulated with TGF-β for the indicated durations. The p-SMAD2 and total SMAD2 (t-SMAD2) levels were analyzed by western blotting. GAPDH, loading control. Representative results from a minimum of three independent experiments are shown. E. Effect of LITATS1 knockdown on E-cadherin, N-cadherin and SNAIL expression in A549 cells. Cells were stimulated with vehicle control (-), SB431542 (SB; 10 μM) or TGF-β (Τβ) for 24 h, and protein expression was analyzed by western blotting. α/β-Tubulin, loading control. Representative results from a minimum of three independent experiments are shown. F"
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "H. In vivo zebrafish extravasation experiments with MDA-MB-231 cells upon LITATS1 knockdown and blockage of TGF-β signaling. Representative zoomed images of the tail fin area are shown in the left panels. Extravasated breast cancer cell clusters are indicated with yellow arrows. Analysis of the extravasated cell cluster numbers in the indicated groups is shown in the right panel. Whole zebrafish image, bar=618.8 μm; zoomed image, scale bar=154.7 μm. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "A, B. Effect of ectopic LITATS1 expression (A) or LITATS knockdown (B) on TβRI expression in MDA-MB-231 cells. Right panel: quantification of relative TβRI protein levels. Vinculin or GAPDH, loading control. Representative blots from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS: 728431
LITATS1: 728431"
Figure legend: "C, D. Analysis of TβRI protein stability (as measured by western blotting) in MDA-MB-231 cells with ectopic LITATS1 expression (C) or LITATS knockdown (D). Cells were treated with CHX (50 µg/mL) for the indicated durations. Quantification of the relative TβRI protein level is shown in the lower panel. GAPDH, loading control. Representative blots from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS: 728431
LITATS1: 728431"
Figure legend: "E. TβRI expression in MDA-MB-231 cells with ectopic LITATS1 expression in the absence or presence of lysosome or proteasome inhibitors. Cells were incubated with vehicle control DMSO (-), the lysosome inhibitor BafA1 (20 nM) or HCQ (20 μM), or the proteasome inhibitor MG132 (5 μM) for 8 h. Vinculin, loading control. Representative results from a minimum of three independent experiments are shown. F. Effect of LITATS1 on TβRI ubiquitination. HEK293T cells were transfected with ectopic expression constructs for HA-Ubiquitin (HA-Ub), caTβRI-FLAG and/or LITATS1. TβRI polyubiquitination was analyzed by western blotting. Representative blots from a minimum of three independent experiments are shown. D"
NCBI gene IDs:
"HA: 
LITATS1: 728431"
Figure legend: "B. Interactions between LITATS1 and TβRI or SMURF2 in MDA-MB-231 cells were detected by the CARPID approach. Cells with stable expression of TurboID-dCasRx were transduced without (Co.) or with (#1 and #2) LITATS1 targeting gRNAs. Cells were stimulated with or without TGF-β (2.5 ng/mL) for 2 h and were then stimulated with biotin (500 μM) for 30 min. Western blotting was performed to detect SMURF2 and TβRI expression in whole-cell lysates (Input) and immunoprecipitates (IP). GAPDH and HA-dCasRx expression levels were measured for equal loading of Input samples and as the negative control or positive control, respectively, for proximity biotinylation in immunoprecipitate (IP) samples. Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"dCasRx: 
TurboID: 
LITATS1: 728431"
Figure legend: "C. Interactions between LITATS1 and TβRI (left) or SMURF2 (right) were analyzed by RIP. MDA-MB-231 cells were stimulated with or without TGF-β (5 ng/mL) for 4 h before RIP. RT‒qPCR was performed to detect LITATS1 expression in immunoprecipitates from MDA-MB-231 cells. IgG was included as the control for immunoprecipitation. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "D. Interactions between LITATS1 and caTβRI or SMURF2 were analyzed by RNA pull-down. Biotinylated 25x poly(A), antisense LITATS1 (LITATS1-AS) or LITATS1 was incubated with lysates from HEK293T cells transfected with the caTβRI-HA or MYC-SMURF2 expression construct. Western blot analysis was performed to detect HA or MYC expression in whole-cell lysates (Input) and immunoprecipitates (IP). Representative blots from a minimum of three independent experiments are shown."
NCBI gene IDs:
"HA: 
MYC: 
SMURF2: 64750
TβRI: 7046
LITATS1: 728431"
Figure legend: "E. In vitro RNA pull-down assays were performed to evaluate the interactions between LITATS1 and SMURF1/2. In vitro-transcribed Antisense LITATS1 (LITATS1-AS) or LITATS1 (LITATS1-S) was incubated with recombinant FLAG-tagged SMURF1 or SMURF2 protein. Western blotting analysis was performed to evaluate FLAG expression in input and IP samples. The amounts of RNA probes used for RNA pull-down were evaluated by agarose gel electrophoresis. Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "G. Effect of LITATS1 overexpression on SMURF2-mediated TβRI polyubiquitination. HEK293T cells were transfected with expression constructs for HA-Ubiquitin (HA-Ub) and caTβRI-FLAG and ectopic expression constructs for SMURF2 and/or LITATS1. Polyubiquitination of TβRI was evaluated by western blotting. Representative blots from a minimum of three independent experiments are shown."
NCBI gene IDs:
"FLAG: 
HA: 
SMURF2: 64750
TβRI: 7046
LITATS1: 728431"
Figure legend: "H. Effect of SMURF2 knockdown on LITATS1-mediated TβRI polyubiquitination. MDA-MB-231 cells with stable HA-Ub expression were transduced with expression constructs for LITATS1 and/or two different SMURF2 shRNAs, as indicated. Polyubiquitination of TβRI was evaluated by western blotting. Representative blots from a minimum of three independent experiments are shown."
NCBI gene IDs:
"HA: 
SMURF2: 64750
LITATS1: 728431"
Figure legend: "A. An in vitro RNA pull-down assay was performed to evaluate the interaction between LITATS1 truncation mutants and SMURF2. Recombinant FLAG-SMURF2 protein was incubated with antisense LITATS1 (LITATS1-AS), LITATS1 (LITATS1-S), or LITATS1 truncation mutants (T1-T4). Western blot analysis was performed to evaluate FLAG expression in immunoprecipitates (IP). The amounts of RNA probes used for RNA pull-down were evaluated by agarose gel electrophoresis. Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "E. SMURF2 expression and localization (as measured by immunofluorescence) upon LITATS1 depletion in A549 cells. DAPI staining was performed to visualize nuclei. Scale bar=23.2 μm. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "F. Effect of LITATS1 knockdown on SMURF2 localization in A549 cells. After subcellular protein fractionation, western blotting was performed to detect SMURF2 expression in whole-cell lysates (Total) and the cytoplasmic (Cyto) and nuclear (Nuc) fractions. The levels of the cytoplasmic marker GAPDH and the nuclear marker Lamin A/C are included to demonstrate subcellular protein fractionation. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "G Activity of PBMCs challenged with ORNs and total S. aureus (Sa) RNA (Myd88d/d, mutant MyD88 expression not impairing LPS driven IL-8 production; n = 2)."
NCBI gene IDs:
"Myd88: 4615
MyD88: 4615"
Figure legend: "I Activity of parental and Unc93b1-/--3ddiTHP-1 cells challenged with ORNs (n = 3)."
NCBI gene IDs:
"Unc93b1: 81622"
Figure legend: "B, C Responsiveness of Unc93b1-/--3ddiTHP1 cells treated with T2.5 and challenged with heat inactivated (hi) bacteria (triangle, decreasing doses; n=3)."
NCBI gene IDs:
"Unc93b1: 81622"
Figure legend: "E, F NF-κB driven relative luciferase activity (Rel. luc. act.) of hTLR8 overexpressing HEK293 and murine RAW264.7 cells, respectively, upon challenges (vector, empty plasmid; n = 3)."
NCBI gene IDs:
"NF-κB: 4790
TLR8: 51311"
Figure legend: "G Transfection of TLR8 siRNA impairs 3ddiTHP1 cell responsiveness (n = 3)."
NCBI gene IDs:
"TLR8: 51311"
Figure legend: "C Responsiveness of Tlr8-/--3ddiTHP1 cells challenged with heat inactivated (hi) bacteria (triangle, decreasing doses; *, p ≤ 0.05; **, p ≤ 0.01) upon TLR2 blockade (T2.5)."
NCBI gene IDs:
"Tlr8: 51311"
Figure legend: "E-H NF-B driven relative luciferase activity (Rel. luc. act.) of hTLR8+ HEK293 cells or cytokine release by PBMCs all transfected with RNAs indicated and additional uridine (U) if indicated (n.p., not performed; vector, empty plasmid)."
NCBI gene IDs:
"NF-B: 4790
TLR8: 51311"
Figure legend: "Figure 2. SPOP localizes to nuclear speckles or other nuclear bodies. Confocal microscopy images of fixed NIH 3T3 cells transiently expressing (A) full length HA-SPOP, (B) GFP-Gli31455, and (C) HA-SPOP+GFP-Gli31-455 are shown. DAPI was used to stain the nucleus, SPOP localization was identified using a anti-HA antibody, nuclear speckle localization was identified using a anti-SC-35 antibody, and Gli31-455 localization was identified via GFP fluorescence. SPOP co-localizes with a marker for nuclear speckles or with the substrate Gli31455. The areas with overlapping HA- and GFP signal contain 75% of the punctate HA signal, and 100% of the punctate GFP signal."
NCBI gene IDs:
"Gli3: 14634
SPOP: 20747"
Figure legend: "(A-B) NIH 3T3 cells were (A) co-transfected with constructs expressing full length SPOP and GFP-Gli31455 or (B) transfected with a construct expressing SC-35-GFP, and GFP fluorescence was monitored in live cells. (A) Snapshots were taken from individual timepoints as noted in the Figure to show photobleaching (at 0 s), recovery, and nuclear body fusion events. The arrow in each panel points to the body that was photobleached and subsequently fuses with another body. These data were not used to calculate the average recovery time as the fusion event precludes accurate measurement of fluorescence recovery. (B) Snapshots at indicated timepoints show a nuclear speckle fusion event. Additional images are shown in Fig EV1."
NCBI gene IDs:
"Gli3: 14634
SPOP: 20747
SC-35: 20382"
Figure legend: "(D-E) Nuclear bodies are close to spherical. (D) Aspect ratios for speckles observed in NIH 3T3 cells transfected with constructs expressing HA-SPOP (151 cells) (D), or GFP-Gli31-455 and HA-SPOP (155 cells) (E) were calculated as described previously (Brangwynne et al, 2011). Representative individual images of cells are shown as insets in each panel."
NCBI gene IDs:
"Gli3: 14634
SPOP: 20747"
Figure legend: "(A) SEC chromatograms of given loading concentrations of SPOP28-359 are shown. The concentrations were normalized to the monomer molecular weight, i.e., identical concentrations of dimeric SPOP ΔBACK (see Fig EV2) and oligomeric SPOP28-359 contain identical numbers of protomers. (B) SEC chromatograms for SPOP constructs defective in self-association in one or both oligomerization domains are shown. Proteins were injected at the same loading concentration (533 µM) and the elution volume of globular molecular weight standards is noted above the graph. (C) SEC chromatograms of SPOP28-359 (200 µM), SPOP mutBACK (718 µM), and mixtures of the two (ratios given in the Figure, SPOP at 200 µM) are shown. The elution volume of globular molecular weight standards is noted above the graph."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(D) Constructs for expressing full-length HA-SPOP or HA-SPOP mutants capable of oligomerization through only one (or neither) of the self-association domains were transfected into NIH 3T3 cells. DAPI was used to stain the nucleus and SPOP localization was identified using an anti-HA antibody. Experiments were performed at least twice on four biological samples. Multiple cells were examined and representative cells are shown. For additional images see Appendix Fig S3. Scale bar represents 5 µm."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(A) Substrate binding was assayed by anisotropy using a fluorescently labeled substrate peptide. All SPOP proteins bound substrate with an affinity similar to that of SPOP28-359 (4-8 µM, see Table 2). Experimental data are shown as circles; the solid lines are fits to eq. 2 (Roehrl et al, 2004)."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(B) In vitro crosslinking assays were performed for SPOP28-359 and each mutant at 30 μM protein with the amide-specific BS3 crosslinker. Crosslinking for SPOP ΔBACK and MATH domain are shown to demonstrate that crosslinking conditions do not lead to non-specific crosslinking of protein species. (C) Crosslinking reactions were performed on whole cell lysates from cells expressing wild type SPOP, SPOP mutBACK, SPOP mutBTB, or SPOP mutBTB-BACK. SPOP1, SPOP2 and SPOPn identify SPOP monomers, dimers and larger species, respectively. For loading levels see Appendix Fig S4."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(A) SV-AUC data for 0.2 nM AF488-SPOP ΔBACK. Fluorescence scans were collected for 12 h and are plotted against distance from the axis of rotation (circles). The data were subjected to standard c(s) analysis in SEDFIT (Schuck, 2000) and the results of this fit are shown (solid lines, rainbow color scheme)."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(B) Experimental weight-average molar mass (Mw) from CG-MALS for SPOP28-359 (orange circles) was fitted to an isodesmic self-association model in which SPOP dimers are the self-associating unit (orange line). The largest SPOP oligomer taken into account was an undecamer of SPOP dimers ((SPOP2)11). The fits from three independent experiments yielded a KD2 of 2.4 ± 0.4 µM. Lines for fits of the data to self-association models that assume formation of individual oligomeric species instead of isodesmic self-association are shown for reference (gray lines)."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(C) Experimental weight-average molar mass (Mw) from CG-MALS data (circles) and fits (solid lines) are depicted for each SPOP construct assayed, showing that a progressive increase in SPOP oligomer size is observed only when both self-association domains are interaction-competent. This Figure is comprised of data shown in Figures 6D, 7B, and Appendix Fig S5A for direct comparison."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "(A) Native MS spectrum of 30 µM SPOP28-359 confirms that SPOP self-associates through addition of dimers. The increase in mass indicates that the dimer is the stable building block within the assemblies."
NCBI gene IDs:
"SPOP: 20747"
Figure legend: "Figure 9. The self-association deficient SPOP mutant mutBTB has a substrate degradation defect in vivo. UAS transgenes were expressed under the control of an epithelial driver C765-Gal4 in Drosophila melanogaster. (A) UAS vector served as a control and yielded a normal wing. Longitudinal veins are denoted by numbers 1-5. Expression of (B) SPOP WT resulted in a modest Hh loss-of function phenotype with fusion of LV3 and LV4 (arrow). (C) mutBTB acts in a dominant-negative manner, as evidenced by a modest Hh gain-of function phenotype with expansion of the LV3-LV4 intervein space. Expression of (D) mutBACK and (E) mutBTB-BACK does not induce a wing patterning defect. ~50 animals each were analyzed from two independent crosses."
NCBI gene IDs:
"UAS: 
Hh: 42737
SPOP: 41704"
Figure legend: "(G) RT-PCR assay showing the efficiency of the atg4daSMO."
NCBI gene IDs:
"atg4da: 492528"
Figure legend: "Immunostaining with (A-C) anti-Pvalb7 and (D-F) anti-zebrinII antibody show loss of cerebellarPurkinje cells in atg4da morphants. (A,D) Control embryos at 4.5 dpf. (B,E) Morphants with mild phenotype show partial loss of cerebellarPurkinje cells. (C,F) Morphants with strong phenotype show either total loss of Purkinje cells or presence of few differentiated neurons, which are laterally located in the cerebellum. (G-I) Labeling of cerebellar granule cells with anti-Vglut1 antibody in control and morphant embryos. (H) Mildly affected morphants show reduced expression of Vglut1 in the cerebellum. (I) Vglut1 expression is strongly reduced in embryos showing severe phenotype. White arrowheads indicate the region of cerebellum. Abbreviations: hb, hindbrain."
NCBI gene IDs:
"atg4da: 492528"
Figure legend: "(D) ACE2 levels were characterized using RNAscope. A representative 3D view of a 232 x 232 μm2 field of view of the AT  layer of an uninfected control at 3 days at air-liquid interface (3C) is shown. The nuclear labelling is indicated in electric indigo and ACE2  mRNA is labelled in spring green LUT. (G) Same as in (D) with infected LoC at 3 dpi (G) shown."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "(A The kinetics of release of SARS-CoV-2  progeny was assessed from samples obtained from a wash of the apical  face of the chip and the vascular effluent (A) collected at each day-post infection. (A) The  number of viral genomes in each sample was quantified using a one-step  qRT-PCR assay for the SARS-CoV-2 N gene. The starting inoculum introduced to the apical side (labelled 'Inn') corresponded to 200-300 PFU. Lines join datapoints from the same  LoC, reconstituted with (green squares) and without (dark blue circles) macrophages. Viral genome numbers within the apical wash showed a declining trend over 3 days of infection. Viral genomes are not detected ('nd') in the vascular effluent for all the chips shown. Data information: In all plots, the bars represent the mean value, and the error bars represent the standard deviation, the solid black line in (A) represents the median."
NCBI gene IDs:
"N gene: 43740575"
Figure legend: "(M) Plot of the fold change in expression of TJP1  (AT and endothelial  layers) and CDH5, CD31, and CD146 (endothelial layers only) in infected LoCs at 1 and 3 dpi (n=3 biological replicates in each case) vs, uninfected controls (n=3 biological replicates). (N) Plot of the fold change in gene expression of  endothelial cell specific coagulation markers - Tissue Factor (F3), Tissue Factor Pathway Inhibitor (TFPI), Thrombomodulin (THBD), Plasminogen Activator Inhibitor (SERPINE1) and von Willebrand Factor (VWF) in infected LoCs  at 1 and 3 dpi (n=3 biological replicates in each case) vs. uninfected controls (n=3 biological replicates). Data information: In all plots, bars represent the mean value and error bars represent the standard deviation. Scale bar = 20 μm. P-values are calculated using a one-way Kruskal-Wallis ANOVA test, * represents p≤0.05, ** represents p≤0.01, ** represents p≤0.001. "
NCBI gene IDs:
"CDH5: 1003
F3: 2152
Tissue Factor: 2152
CD146: 4162
CD31: 5175
Plasminogen Activator Inhibitor: 5054
SERPINE1: 5054
TFPI: 7035
Tissue Factor Pathway Inhibitor: 7035
THBD: 7056
Thrombomodulin: 7056
TJP1: 7082
von Willebrand Factor: 7450
VWF: 7450"
Figure legend: "(A-H) 3D views of 232 x 232 μm2 fields of view of the AT  (A, G) and endothelial  layer (B, H) from infected LoCs reconstituted without macrophages at 1 (A, B) and 3 dpi (G, H). Orf1ab  antisense RNA, S RNA, ACE2  mRNA, and nuclear staining with DAPI  are false-colored pink, amber, spring green, and electric indigo, respectively. (C, D) Zooms corresponding to the region in (A) highlighted with white and yellow boxes, respectively. (C) An example of infection and intracellular replication in a cell with no detectable ACE2 expression (white arrow) as well as nuclear localization of viral RNA (white arrowhead). (D) An example of an uninfected cell with ACE2 mRNA expression (yellow arrow). (E, F) Zooms corresponding to the regions in (B) representing increased nucleic acid staining (hyperplasic) and normal levels of nucleic acid staining highlighted with white and yellow boxes, respectively. (E) Examples of infection of hyperplasic endothelial cells both with and without ACE2 expression (F) Examples of endothelial cell  infection with no ACE2 expression, nuclear localization of viral RNA in an infected endothelial cell is indicated (white arrow)."
NCBI gene IDs:
"ACE2: 59272
Orf1ab: 43740578
S RNA: 100861536"
Figure legend: "(A)  Expression relative to GAPDH  of pro-inflammatory cytokines  (TNFA, IL1B, IL6), interferon genes (IFNB, IFNL1, IFNL3) and interferon stimulating genes (IP10, ISG15) in the AT and endothelial  layer of infected LoCs at 1 dpi reconstituted without macrophages (n=3 biological replicates for  IFNB, IFNL1 and  IFNL3 in the AT layer, n=4 biological replicates for all other data). (B) Fold-change in the markers in (A) relative to uninfected controls (n=3 biological replicates) (data in Appendix Fig. S12) at the same timepoint. The bar represents the mean, and the error bars the standard deviation. ( Data information: The bar represents the mean, and the error bars the standard deviation. P-values are calculated using a Kruskal-Wallis One-Way ANOVA Test, * represents p≤0.05, ** represents p≤0.01, ** represents p≤0.001."
NCBI gene IDs:
"IP10: 3627
GAPDH: 2597
IFNB: 3456
IFNL1: 282618
IFNL3: 282617
IL1B: 3553
IL6: 3569
ISG15: 9636
TNFA: 7124"
Figure legend: "(D) Expression of IL6R  and ADAM17 relative to GAPDH in the  AT  and endothelial layer of infected LoCs at 1 dpi (n=3 biological replicates for the AT layer and n=4 biological replicates for  endothelial layer). (E) Fold-change in expression  of IL6 and ADAM17 in the epithelial and endothelial layers of infected LoCs at 1 dpi relative to uninfected controls (n=3 biological replicates for both layers). ( Data information: The bar represents the mean, and the error bars the standard deviation. P-values are calculated using a Kruskal-Wallis One-Way ANOVA Test, * represents p≤0.05, ** represents p≤0.01, ** represents p≤0.001."
NCBI gene IDs:
"ADAM17: 6868
GAPDH: 2597
IL6R: 3570
IL6: 3570"
Figure legend: "(F-I) 3D views of representative 232 x 232 μm2 fields of view of the AT (F, H) and endothelial layer (G, I) from infected LoCs reconstituted with macrophages at 1 (F, G) and 3 dpi (H, I). IL6 mRNA, and nuclear staining are indicated with the chartreuse and electric indigo LUTs, respectively."
NCBI gene IDs:
"IL6: 3569"
Figure legend: "Immunoblot with anti-HA Abs of lysates from parental (Ctrl) and tagged lines (TgCRMPa-HA3 and TgCRMPb-HA3) together with inducible-knockdown lines (TgCRMPa-HA3_iKD and TgCRMPb-HA3_iKD) treated with ATc for 0, 24 or 48h. TgROP5 was used as loading control. Two close bands around 300kDa were detected for TgCRMPa. A ~820kDa protein, corresponding to the predicted size for TgCRMPb, was observed together with a ~130kDa band."
NCBI gene IDs:
"HA3: 
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "Quantification of microneme secretion in TgCRMPa- and TgCRMPb-depleted tachyzoites was measured by detecting the processed form (arrowhead) of TgMIC2 (arrow) in the media. Control, TgCRMPa_iKD and TgCRMPb_iKD parasites, ATc-treated (+) and untreated (-), were stimulated with propranolol to release microneme contents. Blots were probed with anti-MIC2 (secretion of micronemes) and anti-GRA3 (constitutive secretion of dense granules). P: Parasites pellet. Sup: Supernatant from untreated parasites. Sup+Prop: Supernatant from parasites treated with propranolol. The results are representative of two independent experiments."
NCBI gene IDs:
"MIC2: 7894517
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "Co-immunoprecipitation of TgCRMPa and TgCRMPb. Lysates from parasites co-expressing TgCRMPa-FLAG3 and TgCRMPb-HA3 were split and incubated with either anti-FLAG (left panels) or anti-HA beads (right panels). Eluates were subjected to SDS-PAGE and immunoblotted with anti-HA and anti-FLAG Abs. Untagged, TgCRMPa-FLAG3 and TgCRMPb-HA3 parasites were used as controls. TgCRMPs robustly associate with each other."
NCBI gene IDs:
"FLAG3: 
HA3: 
CRMPa: 7894778
CRMPb: 7897818"