EMBO/SourceData huggingface dataset:
Figure legend: "(A) Real-time quantitative reverse transcription polymerase chain reaction  (PCR) (qRT-PCR) analysis of the differential abundance of selected bacterial taxa in stool  samples of  ileum  and colon of Villin-Cre and COPS8-lox alleles expressing (COPS8fl/fl ) and COPS8  knockout (KO) (COPS8ΔIEC) mice. Error bars indicate the mean ± SD from three biological replicates per genotype. **P&lt;0.01, NS- nonsignificant using Student's t-test. ("
NCBI gene IDs:
"COPS8: 108679
Cre: 2777477
Villin: 22349"
Figure legend: "Mice  were treated by 2% dextran sodium sulfate  (DSS) in drinking water for 7 days. (F) Representative  hematoxylin and eosin (HE)  stained sections of colon  and cecum from COPS8fl/fl and COPS8ΔIEC mice after DSS-indcued colitis (day 9). Scale bar 100μm, representative data from five biological replicates per genotype. (G) Histopathologically scored sections of distal colon; cecum and colon length were analyzed from COPS8fl/fl and COPS8ΔIEC mice after  DSS-indcued colitis (day 7). Data are represented as mean ± SEM from five biological replicates per genotype. **P&lt;0.01 using Student's t-test. ("
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "Mice  were treated by 2% dextran sodium sulfate (DSS) in drinking water for 7 days. (H) Cytokine levels (interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α)) in the  colon  collected on day 7 in colitis induced COPS8fl/fl and COPS8ΔIEC mice. Data are represented as mean ± SEM from five biological replicates per genotype. *P&lt;0.05, **P&lt;0.01, NS- non-significant using Student's t-test."
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "Mice were treated by 2% dextran sodium sulfate (DSS) in drinking water for 7 days. (I) The frequency of CD11b+Ly6C+ and CD11b+Ly6G+ cells in colonic lamina propria  (cLP) of COPS8fl/fl and or COPS8ΔIEC mice  with DSS-induced colitis. The column graph represents percentage of Ly6C+ and Ly6G+ cells, presented as mean ± SEM from three biological replicates per genotype. *P&lt;0.05, NS- non-significant using Student's t-test. (J) Representative  FACS plots and percentage of intracellular staining of  Forkhead box protein P3 (FOXP3), Interferon gamma (IFN-γ) and IL-17A in CD3+CD4+  T cells from  colonic lamina propria  of COPS8fl/fl or COPS8ΔIEC mice with DSS-induced  colitis. The column graph represents percentage of Treg+ and ratio of Th1/Th17 cells, represented as mean ± SEM from three biological replicates per genotype. *P&lt;0.05 using Student's t-test."
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "(I) Real-time quantitative reverse transcription polymerase chain reaction  (qRT-PCR) analysis of the expression of genes encoding inflammatory cytokines in the ileum. COPS8fl/fl and COPS8ΔIEC mice were either untreated (phosphate buffered saline (PBS)) or treated with the antibiotic cocktail (Abx) for 3 months. Data are mean ± SEM from three biological replicates. **P&lt;0.01, NS- non-significant using one-way ANOVA."
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "(B) Representative image showing changes in  colon morphology and length following DSS-induced  colitis  with concurrent treatment with MBELNs COPS8fl/fl and COPS8ΔIEC mice. Representative data from seven biological replicates per genotype."
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "(F)  Expression of anti-microbial peptide (AMPs) in the  colon  of COPS8fl/fl and  COPS8ΔIEC mice  while being treated with  MBELNs by oral administration. Data are mean ± SEM from three biological replicates per group. *P&lt;0.05, **P&lt;0.01, NS- non-significant using one-way ANOVA."
NCBI gene IDs:
"COPS8: 108679"
Figure legend: "(I-J) Transcriptional expression of  nuclear factor kappa B (NF-κB) (I) and inhibitor of nuclear factor kappa-β kinase (IKK-β) (J) mRNA using Real-time quantitative reverse transcription Polymerase chain reaction (qRT-PCR) in colonic epithelial cells upon treatment with MBELNs  in addition to  DSS. Data are mean ± SEM from three biological replicates per group. **P&lt;0.01, NS- non-significant using one-way ANOVA."
NCBI gene IDs:
"IKK-β: 16150
inhibitor of nuclear factor kappa-β kinase: 16150"
Figure legend: "Proliferation curves of parental and AGO2KO HeLaS3 cells are plotted (n=6 experimental replicates)."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "Colony-forming activity of HeLaS3 and AGO2KO cells was determined by colony-formation assay (n=3 experimental replicates)."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "Tumour growth curves of xenografts derived from HeLaS3 or AGO2KO cells are shown as tumour diameter at different time points (n=5 mice for each group)."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "HeLaS3 cells were transduced with a lentiviral vector coding for human TERC (HeLaS3_TERC) or for GFP, as a control (HeLaS3_GFP). The relative expression of TERC was measured by RT-qPCR. HPRT1 was used as internal control (n = 3 experimental replicates)."
NCBI gene IDs:
"GFP: 
HPRT1: 
TERC: 7012"
Figure legend: "The relative expression of terc-sRNA was measured in HeLaS3_TERC and HeLaS3_GFP by RT-qPCR. RNU44 was used as internal control (n = 3 experimental replicates)."
NCBI gene IDs:
"GFP: 
RNU44: 
terc: 7012
TERC: 7012"
Figure legend: "Average telomere length was measured from genomic DNA of parental and AGO2KO HeLaS3, by qPCR amplification of telomere repeats (T) and multicopy gene Alu (R), as a reference. The relative telomere length (T/R) was plotted (n=3 experimental replicates)"
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "Representative images of metaphase spreads obtained from HeLaS3 and AGO2KO cells stained for telomeric sequences and centromere 2 alphoid DNA."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "Representative telomere length distributions in parental and AGO2KO HeLaS3. Average telomere length and the standard deviation, as well as the total number of telomeres analyzed, are indicated. Notice the marked shift towards short telomeres in AGO2KO cells compared with the parental HeLa S3 cell line."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "Telomerase activity was detected by TRAP in parental HeLaS3 and AGO2KO cells. 293T cells were used as a positive control. As negative controls, telomerase was heat- inactivated in cell extracts, in the last lane no cells were added in lysis buffer. Intensity of the telomerase products (6 bp-ladder) was determined by ImageLab Software (Biorad) and normalized with the intensity of the Internal Control (IC) (n=3 experimental replicates)."
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "AGO2KO cells were transduced with a lentiviral vector coding for FLAG-HA-tagged AGO2 (AGO2KO_FH AGO2) or for GFP as a control (AGO2KO_GFP). Quantification of telomerase activity in AGO2KO_FH AGO2 and AGO2KO_GFP, as assessed by TRAP, was plotted (n=3 experimental replicates)."
NCBI gene IDs:
"FLAG: 
GFP: 
HA: 
AGO2: 27161"
Figure legend: "HeLaS3 cells were transduced with a lentiviral vector coding for FLAG-HA-tagged AGO2 (HeLaS3_FH AGO2) or for GFP as a control (HeLaS3_GFP). Quantification of telomerase activity in HeLaS3_FH AGO2 and HeLaS3_GFP, as assessed by TRAP, was plotted (n=3 experimental replicates)."
NCBI gene IDs:
"FLAG: 
GFP: 
HA: 
AGO2: 27161"
Figure legend: "The relative expression of TERC was measured by RT-qPCR from total RNA of HeLaS3 and AGO2KO cells. HPRT1 was used as internal control (n = 3 experimental replicates). qPCR of 3'-extended TERC transcript in cDNA from HeLaS3 and AGO2KO cells generated using random hexamers. HPRT1 was used for normalization (n=3 experimental replicates). qPCR of TERC transcript in cDNA from HeLaS3 and AGO2KO cells generated using oligo(dT)10 priming. HPRT1 was used for normalization (n=3 experimental replicates)."
NCBI gene IDs:
"HPRT1: 
AGO2: 27161
TERC: 7012"
Figure legend: "Whole cell extract of HeLaS3 and AGO2KO was analyzed by western blot for the presence of TERT protein. GAPDH was used as loading control. Data are representative of 4 independent experiments. TERT protein level was quantified by ImageLab Software (Biorad). GAPDH was use as internal control (n=4 experimental replicates)"
NCBI gene IDs:
"AGO2: 27161"
Figure legend: "HeLaS3 and AGO2KO cell extracts (n=3), were immunoprecipitated using an anti-TERT antibody or IgG as mock IP. TERC and HOTAIR (as a negative control) abundance was assessed by RT-qPCR. HPRT1 was used for normalization and enrichment in TERT-RIP as compared to IgG RIP was plotted."
NCBI gene IDs:
"AGO2: 27161
HOTAIR: 100124700
TERC: 7012"
Figure legend: "AGO2KO_FH AGO2 and AGO2KO_GFP cell extracts (n=3 experimental replicates) were immunoprecipitated using an anti-TERT antibody or IgG as mock IP. TERC and HOTAIR (as a negative control) abundance was assessed by RT-qPCR. HPRT1 was used for normalization and enrichment in TERT-RIP as compared to IgG RIP was plotted."
NCBI gene IDs:
"GFP: 
AGO2: 27161
HOTAIR: 100124700
TERC: 7012"
Figure legend: "HeLaS3 whole-cell extract was immunoprecipitated using anti- AGO2 antibody or IgG, as mock IP. TERC RNA enrichment in AGO2 RIP as compared to IgG RIP was assessed by RT-qPCR . 7SK RNA was used for normalization (n=4 experimental replicates)."
NCBI gene IDs:
"TERC: 7012"
Figure legend: "RIP assay was performed from HeLaS3_FH AGO2 and HeLaS3_GFP cell extract using anti-HA antibody or IgG, as negative control, followed by TERC detection by RT-qPCR. 7SK RNA was used for normalization (n=3 experimental replicates)."
NCBI gene IDs:
"GFP: 
AGO2: 27161
TERC: 7012"
Figure legend: "HeLaS3 cells were transduced with a lentiviral vector coding for wild-type TERC (HeLaS3_TERCwt), TERC mutated in position 313-340 (HeLaS3_TERC (313-340mut)) or TERC delated in position 12-31 (HeLaS3_TERC (Δ 12-31)). Whole-cell lysates from HeLaS3_TERCwt, HeLaS3_TERC(313-340mut) and HeLaS3_TERC (Δ 12-31) was immunoprecipitated using an anti-AGO2 antibody in order to assessed the impact of TERC mutations on AGO2 binding. For each experiment the enrichment of ectoTERC as compared to 7SK RNA in RIP samples was normalized on the amount of ectoTERC and 7SK RNA in input samples. Association of AGO2 with TERC(313-340mut and TERC (Δ 12-31) was compared to the association with TERCwt (n=3 experimental replicates)."
NCBI gene IDs:
"TERC: 7012"
Figure legend: "HeLaS3 and AGO2KO cells were transfected with a synthetic terc-sRNA or an siRNA with no target in humans (siCtl), as a control. Four days after transfection, telomerase activity in 100- and 50 cell-lysate was assessed by TRAP. Intensity of the telomerase products (6 bp-ladder) was determined by ImageLab Software (Biorad) and normalized with the intensity of the Internal Control (IC), (n=4 experimental replicates)"
NCBI gene IDs:
"AGO2: 27161
terc: 7012"
Figure legend: "D. Control and  biotin-treated  HEK293T cells stably expressing V5-BirA* and V5-BirA*-AURKA were lysed and biotinylated proteins were precipitated  by   streptavidin beads. The initial sample (initial) and captured biotinylated  proteins (pulldown) were run on an SDS gel and  immunoblotted with the fluorescent streptavidin and antibodies against  V5 and vinculin (loading control)."
NCBI gene IDs:
"V5: 
AURKA: 6790
BirA: 948469"
Figure legend: "F. AURKA co-localize with PCM1  at the  peripheral  satellite clusters upon chemical dimerization of satellites with Kif5b motor domain.  U2OS  cells were co-transfected with  GFP-PCM1-FKBP and HA-Kif5b(1-269 a.a.)-FRB and treated with 500 nM rapamycin for 1 hour followed by fixation at 6 hours. Cells that were not treated with rapamycin  were processed in parallel as a control. Fixed cells were  stained  with antibodies against GFP, AURKA and gamma-tubulin. DNA was stained with DAPI. Cell edges are outlined. Scale bar, 10 μm. G. Quantification of  pericentrosomal levels of AURKA and gamma tubulin for F. n&gt;25 cells per experiment. Data represent mean value from two experiments per condition ± SEM (****p &lt; 0.0001; **p &lt; 0.01; Unpaired Student's t-test.). "
NCBI gene IDs:
"GFP: 
HA: 
FKBP: 11328///23307///23770///2288///2289
Kif5b: 3799
PCM1: 5108"
Figure legend: "A, B. Representative  images and quantification of basal body  levels of (A) AURKA and (B) phospho-AURKA (p-AURKA) in control and  PCM1-depleted cells. RPE1 cells were transfected with control or PCM1 siRNA #1 or siRNA #2 for 48 h. Following 24 h serum  starvation, cells were fixed and stained with the indicated antibodies.  Centrosomal AURKA and p-AURKA fluorescence intensities were measured from maximum projections, and average means of the levels in control cells were normalized to 1. n&gt;500 cells per experiment. Data for (A) AURKA and (B) p-AURKA represent mean value from three experiments per condition ± SEM (****p &lt; 0.0001, Unpaired Student's t-test.) Scale bar, 10 μm"
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "D. Cycloheximide chase experiment for quantification of AURKA half-life. Cells were transfected with control or PCM1 siRNA#1 for 48 h, then treated with 200 nM cycloheximide along with serum starvation for indicated time points. AURKA intensities were quantified by immunoblotting for AURKA and vinculin  (loading control) and AURKA levels were normalized to vinculin levels. Data represent mean value from three experiments per condition ± SEM (**p &lt; 0.01, Unpaired Student's t-test)"
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "A. Representative  immunofluorescence images of  cilium assembly experiments in control and PCM1-depleted RPE1  cells treated with DMSO  or MLN8237. Cells were transfected with control or PCM1  siRNA#1 for 48 h and treated with  DMSO (vehicle control) or 0.5 μM MLN8237  in  serum starvation medium for 24 h. Cells were fixed and immunostained for the primary cilium with acetylated tubulin antibody (Acet-tub) and the centrosome with gamma-tubulin  antibody. DNA was stained  with DAPI. Scale bar, 10 μm. B. Quantification of  ciliogenesis  efficiency for A. n&gt;100 cells per experiment. Data represent mean value from three experiments per condition ± SEM (***p &lt; 0.001; **p &lt; 0.01; ns, non-significant, Unpaired Student's t-test.). C. Quantification of cilium length for A. n&gt;100 cells per experiment. Data represent mean value from three experiments per condition ± SEM (****p &lt; 0.0001; ns, non-significant, Unpaired Student's t-test.). "
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "D. Representative  immunofluorescence images of cilium assembly experiments in control and PCM1-depleted  RPE1  cells treated with DMSO or tubacin. Cells were transfected with control or  PCM1  siRNA#1 for 48 h and treated with DMSO or 2 μM tubacin in serum starvation medium for 24 h. Cells were fixed and immunostained for the  primary cilium  with Arl13b  and acetylated tubulin antibody (Acet-tub) and the centrosome with gamma-tubulin antibody. DNA was stained with  DAPI. Scale bar, 10 μm E. Quantification of ciliogenesis efficiency for D. n&gt;100 cells per experiment. Data represent mean value from three experiments per condition ± SEM (*p &lt; 0.05; ns, non-significant, Unpaired Student's t-test). "
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "F. Representative immunofluorescence images and quantification of IFT88 levels at the centrosome and primary cilium. RPE1 cells were transfected with control or  PCM1 siRNA#1 for 48 h,  serum starved for 24 h and treated with DMSO or 0.5 μM MLN8237. Cells were fixed and immunostained with antibodies against  IFT88, acetylated tubulin and  gamma tubulin.  DNA was stained with DAPI. Scale bar, 10 μm. G. Quantification of  IFT88 centrosomal intensity and  ciliary concentration for F.  IFT88 fluorescence intensities at the centrosome and axoneme were measured from maximum projections, and average means of the levels in control cells were normalized to 1. Ciliary  concentration was calculated by dividing ciliary fluorescence intensity to the cilium length. n&gt;100 cells per experiment. Data represent mean value from three experiments per condition ± SEM (***p &lt; 0.001; ****p &lt; 0.0001, Unpaired Student's t-test) "
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "H, I. Quantification of cilium  disassembly and centrosomal AURKA levels after  serum stimulation. RPE1 cells were transfected with control or PCM1 siRNA#1 for 48 h, serum starved for 24 h and treated with  DMSO or 0.5 μM MLN8237 in  serum stimulation medium for 2 h and 24 h. Cells were fixed and  immunostained with antibodies against   acetylated tubulin  and gamma tubulin, and percentage of ciliated cells were quantified. x-axis indicates the hours after serum stimulation. (H) n&gt;100 cells per experiment. Data represent mean value from three experiments per condition ± SEM. (I) n&gt;95 cells per experiment. Data represent mean value from three experiments per condition ± SEM (***p &lt; 0.001; ****p &lt; 0.0001, Unpaired Student's t-test)."
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "RNA was extracted from young fertile adults fed ad libitum or fasted for 6 h. ddCts (delta delta cycle thresholds) were calculated normalizing to ama-1 and the efficiency of the primer sets as previously described. Means+s.e.m. are depicted. lipl-1 to lipl-5, n = 6 independent experiments. lipl-6 to lipl-8, n = 3independent experiments. Significant t-test derived P-values are indicated. WT, wild type."
NCBI gene IDs:
"ama-1: 
lipl-1: 179771
lipl-5: 178563
lipl-6: 179081
lipl-8: 190108"
Figure legend: "(b) Acid lipase activity, measured in 1-day adult whole lysates, shows that lipl-1lipl-3 double mutant animals have reduced acidic lipolytic capacity. Mean±s.d. are presented relative to wild type (WT); significant differences are indicated; n = 4 independent experiments."
NCBI gene IDs:
"lipl-1: 179771
lipl-3: 178572"
Figure legend: "(c) Oil Red O (ORO) staining and fatty acid methyl ester (FAME) analyses of wild-type and lipl-1(tm1954) lipl-3(tm4498) double mutant L3 larvae show that lysosomal lipases regulate cytosolic fat stores (see Fig. 2d for adult measurements). Means±s.e.m. are presented relative to wild type; significant differences are indicated; n = 3 independent experiments. (d) ORO quantification of wild-type and lipl-1(tm1954) lipl-3(tm4498) double mutant young adults fasted for 4 h shows that LIPL-1 and LIPL-3 contribute to fat mobilization following fasting. (Mean percentage ± s.e.m. relative to fed wild type). Wild-type fasted worms show 20% less ORO signal than animals fed ad libitum (P≤0.001), whereas lipl-1(tm1954) lipl-3(tm4498) double mutant worms show 7% reduction in ORO signal (a difference that is not significant to well-fed fat levels at P≤0.01 but it is significant at P≤0.05). n = 3 independent experiments."
NCBI gene IDs:
"lipl-1: 179771
LIPL-1: 179771
lipl-3: 178572
LIPL-3: 178572"
Figure legend: "(e) Representative transmission electron microscopy images of well-fed and 6 h-fasted wild-type and lipl-1lipl-3 mutant animals (11,500×) show that the lipl mutants have more lipid-droplet stores in ad libitum fed and fasted conditions. Quantification of vesicle number and size is depicted as mean+s.e.m., n = 4 independent experiments."
NCBI gene IDs:
"lipl-1: 179771
lipl-3: 178572"
Figure legend: "(g) ORO staining and quantification of wild-type and lipl-1(tm1954) lipl-3(tm4498) double mutant young adults treated post-developmentally (from L4) with RNAi against the essential autophagy genes lgg-1 and lgg-2 or vector control show that lipl-1 lipl-3 and the autophagy genes lgg-1 lgg-2 are in the same fat regulatory pathway. (Mean percentage+s.e.m. relative to wild type on vector control is depicted.) n = 4 independent experiments."
NCBI gene IDs:
"lgg-1: 174050
lgg-2: 177989
lipl-1: 179771
lipl-3: 178572"
Figure legend: "(a) Expression of lipl genes in well-fed mxl-3(ok1947) and mxl-3(tm2580) young adults normalized to same age well-fed wild-type (WT) worms shows that mxl-3 loss of function is sufficient to induce lipl-1 to 3 and lipl-5. Mean ddCts+s.e.m. are depicted. n = 6 independent experiments for lipl-1 to lipl-5, and n = 4 independent experiments for lipl-6 to lipl-8."
NCBI gene IDs:
"lipl-1: 179771
lipl-5: 178563
lipl-6: 179081
lipl-8: 190108
mxl-3: 181457"
Figure legend: "(b) L4 larvae were fasted and RNA was extracted at the indicated times. Mean ddCts+s.e.m. show that the response mxl-3 orchestrates is transient. Time 0, n = 6 independent experiments; time 3-12 h, n = 4 independent experiments; time 18-24 h, n = 2 independent experiments."
NCBI gene IDs:
"mxl-3: 181457"
Figure legend: "(d) ChIP-quantitative PCR (ChIP-qPCR) analysis of well-fed and 6 h fasted mixed-stage worms expressing MXL-3::GFP presented as Ct in αGFP immunoprecipitated DNA normalized to input DNA and relative to a mock promoter region (CACTAT site −88 of ama-1 gene) shows that MXL-3 vacates the lipl promoters during early fasting. Three sets of primers surrounding CACGTG target sites found up to 500 bp of the ATG of the lipl-1 and lipl-3 genes were used. A representative experiment is presented; see raw data of two independent experiments in Supplementary Table S9."
NCBI gene IDs:
"ama-1: 
lipl-1: 179771
lipl-3: 178572"
Figure legend: "(e) Wild-type or lipl-1(tm1954) lipl-3(tm4498) double mutant animals grown on control RNAi plates were transferred as L4 larvae to control or mxl-3 RNAi plates, incubated for 12 h at 20 °C, and processed for total FAMEs; as a reference, an aliquot of wild-type young adults on control RNAi bacteria was fasted for 6 h. FAME quantification (mean percentage of fed wild type ±s.e.m.) shows that acute mxl-3 inactivation, as fasting, reduces fat stores in a lipl-dependent manner. n = 3 independent experiments. NSD, P-value&gt;0.05."
NCBI gene IDs:
"lipl-1: 179771
lipl-3: 178572
mxl-3: 181457"
Figure legend: "(a) Transcriptional levels of hlh-30 and the mxl-3-dependent lipl genes measured in 5 h fasted wild-type (WT) or hlh-30(tm1978) young-adult worms depicted as mean ddCt+s.e.m. relative to wild-type ad libitum-fed worms show that hlh-30 is induced following fasting and HLH-30 induces lysosomal lipolysis. N = 4 independent experiments for lipl data, n = 2 independent experiments for hlh-30 mRNA. HLH-30 deficiency fully abrogates lipl-2, 3 and 5 (P0.0001), and impairs lipl-1 (P0.01) transcriptional activation following fasting."
NCBI gene IDs:
"hlh-30: 177157
HLH-30: 177157
lipl-1: 179771
lipl-2: 185840
mxl-3: 181457"
Figure legend: "(b) Animals treated from late L3 stage with RNAi against TOR or vector control were harvested as young adults. Mean+s.e.m. of three independent experiments shows that inhibition of TOR is sufficient to induce hlh-30 transcription."
NCBI gene IDs:
"hlh-30: 177157
TOR: 189606///189605"
Figure legend: "(c) L3 animals carrying the rescuing construct hlh-30P::HLH-30::mGFP::hlh-30 3′UTR (mGFP, monomeric GFP, and UTR, untranslated region), well fed or fasted for 8 h show that HLH-30 is enriched in intestinal nuclei of fasted worms (exposure time: well fed, 500 ms; fasted, 100 ms)."
NCBI gene IDs:
"hlh-30: 177157"
Figure legend: "(d) ORO staining of wild-type or hlh-30(tm1978) young adults fasted for 8 h reveals that hlh-30 is required for optimal lipid mobilization on fasting. Mean percentages+s.e.m. are shown below relative to well-fed worms treated in parallel, n = 3 independent experiments."
NCBI gene IDs:
"hlh-30: 177157"
Figure legend: "(e) mxl-3 and hlh-30 transcriptional levels in wild-type, hlh-30(tm1978) and mxl-3(ok1947) mutants in the basal and fasted states show that mxl-3 transcription is hlh-30 independent, and hlh-30 transcription is mxl-3 independent. Expression is presented relative to wild-type fed animals as mean ddCt+s.e.m. No significant differences relative to wild type in the same feeding state were observed, n = 3 independent experiments."
NCBI gene IDs:
"hlh-30: 177157
mxl-3: 181457"
Figure legend: "(g) Transcriptional levels of the mxl-3-dependent lipl genes in wild-type, mxl-3(ok1947), hlh-30(tm1978) or mxl-3(ok1947);hlh-30(tm1978) double mutant young-adult worms show that hlh-30 suppresses the constitutive-induction-of-the-lipl-genes phenotype of mxl-3 mutant animals (mean ddCt+s.e.m. relative to wild-type ad libitum-fed worms). n = 3 independent experiments."
NCBI gene IDs:
"hlh-30: 177157
mxl-3: 181457"