EMBO/SourceData huggingface dataset:
Figure legend: "(A) Immunofluorescence (IF) staining showing Siglec-F and Iba1 localization in hippocampus CA3 region of 3wk induced CK (top) or CK-p25 (bottom) mice. Scale bars = 50 μm. Colors are: Magenta = Iba1, Cyan = Siglec-F. Images are 60x super-resolution max z-stack projections taken from coronal slices. (B-C) Percent area coverage of (B) Iba1 and (C) Iba1-proximal Siglec-F between CK and CK-p25 mice across hippocampal regions. Bars indicate mean ± 95% CI; n = 3-4 animals; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided. "
NCBI gene IDs:
"p25: 12569"
Figure legend: " (A) Confluency doubling times quantified from BV-2 cells with stable retroviral expression of Siglec-F and related human Siglec constructs. Plots indicating doubling time (normalized to 2xY-&gt;F constructs) estimated from Incucyte bright-field images. Cells grown in media alone or + 50 mU sialidase (+SA). Bars indicate mean ± 95% CI; n = 3-12 replicates; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided. Dotted line indicates mean of 2xY-&gt;F groups. "
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "(B) Flow cytometry quantification of cell live / dead markers Annexin-V and propidium iodide on BV-2 cells with stable retrovirus expression of Siglec-F constructs. % parent values are shown for early apoptotic (Annexin-V+;PI‑), late apoptotic (Annexin-V+;PI‑), and necrotic (Annexin-V-;PI+) populations. Bars indicate mean ± SD; n = 8 replicates."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: " (C) Confluency doubling times quantified from BV-2 cells with dox-inducible expression of Siglec-F constructs. Plots indicating doubling time (normalized to 2xY-&gt;F constructs) estimated from Incucyte bright-field images. Bars indicate mean ± SD; n = 8 replicates; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided. Dotted line indicates mean of 2xY-&gt;F group. "
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "(F) Expression levels of C1qa, IL-1β, and β-actin by qPCR analysis in BV-2 cells with inducible Siglec-F expression after 48 hours of treatment with 500 ng/mL doxycycline. Fold Change values are equal to 2ΔΔCq normalized relative to GADPH mRNA levels. Bars indicate mean ± 95% CI; n = 6 replicates; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided. Dotted line indicates mean of 2xY-&gt;F group."
NCBI gene IDs:
"β-actin: 11461
C1qa: 12259
GADPH: 14433
IL-1β: 16176
Siglec-F: 233186"
Figure legend: "(G) Western blot showing pro-IL-1β and β-tubulin in BV-2 cells induced to express Siglec-F at 0 and 48 hours."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "(B-C) Volcano plots showing phosphotyrosine peptides that were (B) increased or (C) decreased after Siglec-F expression. Plots show Siglec-F compared to 2xY-&gt;F mutant data integrated across all three runs. Protein names are shown for altered peptides. Labels are only shown for peptides with maximum directional change from each protein. Labels colored according to GO terms: magenta = signal transduction, red = endosome, cyan = cell adhesion, yellow = cytoskeleton, green = metabolism."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: " (D) Heatmap showing phosphosites in CK-p25 which overlapped in their directional change with BV-2 Siglec-F-associated sites. Colors indicate fold change relative to 2xY-&gt;F controls. "
NCBI gene IDs:
"p25: 12569
Siglec-F: 233186"
Figure legend: " Heatmap showing phosphosites from CD33, Siglec-5, and Siglec-8 overexpression that were increased with Siglec expression and shared their directional change with Siglec-F. Siglec-F column indicates average of all untreated Siglec-F replicates. Color scale is same as (D). "
NCBI gene IDs:
"CD33: 945
Siglec-5: 8778
Siglec-8: 27181
Siglec-F: 233186"
Figure legend: " Heatmap showing phosphosites from CD33, Siglec-5, and Siglec-8 overexpression that were decreased with Siglec expression and shared their directional change with Siglec-F. Siglec-F column indicates average of all untreated Siglec-F replicates. Color scale is same as (D). "
NCBI gene IDs:
"CD33: 945
Siglec-5: 8778
Siglec-8: 27181
Siglec-F: 233186"
Figure legend: "(G) Western blot quantification of myc, SHP-2, and SHP-1 from myc co-IP eluates. Co-IP lysates were prepared from BV-2 cells stably expressing Siglec-F and optionally treated with 1 μM SHP099."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "(B-C) Flow cytometry quantification of fluorescent monomeric Aβ uptake and Siglec-F expression for (B) Siglec-F and (C) Siglec-F 2xY-&gt;F. Siglec-expressing cells were plated with empty vector control cells in the same well alongside fluorescent substrates."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "(G) pHrodo Dextran uptake in BV-2 cells with stable expression of Siglec-F estimated by Incucyte measurements. Bars indicate mean ± 95% CI; n = 6 replicates; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided."
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: " (H) pHrodo dextran uptake in BV-2 cells stably expressing Siglec-F with 0 - 2.5 μM SHP099 treatment. Data is from the same experiment as (G). "
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: " (I) Confluency doubling times for BV-2 cells with stable expression of Siglec-F with 0 - 2.5 μM SHP099 treatment. Data is from the same experiment as (G). Bars indicate mean ± 95% CI; n = 4 replicates; *: p &lt; 5e-2, **: p &lt; 1e-2, ***: p &lt; 1e-3, ****: p &lt; 1e-4; ns: not significant, using unpaired Student's t-test, two-sided. Dotted line indicates mean of untreated 2xY-&gt;F group. "
NCBI gene IDs:
"Siglec-F: 233186"
Figure legend: "A. The expression of EP subtypes in bone marrow myeloid cells and colon tumor myeloid cells. The short black lines indicate individual expressions, the blue and yellow lines indicate the average expression level in each condition. The grey dotted lines represent the overall average between Ptger1 and Ptger3 or Ptger2 and Ptger4."
NCBI gene IDs:
"Ptger1: 19216
Ptger2: 19217
Ptger3: 19218
Ptger4: 19219"
Figure legend: "Relative expression of selected M1, M2 markers on flow-sorted M1 macrophages (D), M2 macrophages (E) from CT26 tumors. Data are normalized to β-actin expression levels (n = 5)."
NCBI gene IDs:
"β-actin: "
Figure legend: "M-CSF induced mouse bone marrow derived macrophages (BMMs) were stimulated with 50 ng/mL mouse recombinant IFN-γ (A) in the absence or presence of PGE2 ± varying concentrations of TP-16 for 12 hr. The mRNA levels of selected M1 markers (Cxcl10 and Tnfa) were measured by q-PCR. Data are normalized to β-actin expression levels (n=3)."
NCBI gene IDs:
"β-actin: 
Cxcl10: 15945
Tnfa: 21926"
Figure legend: "B. M-CSF induced mouse bone marrow derived macrophages (BMMs) were stimulated with 20 ng/mL mouse recombinant IL-4 (B) in the absence or presence of PGE2 ± varying concentrations of TP-16 for 12 hr. The mRNA levels of selected M2 markers (Arg-1 and Ym-1) were measured by q-PCR. Data are normalized to β-actin expression levels (n=3)."
NCBI gene IDs:
"β-actin: 
Arg-1: 11846
Ym-1: 12655"
Figure legend: "D. Bone marrow cells were treated with GM-CSF/IL-6 for 6 days to induce MDSCs and thereafter, with PGE2 ± varying concentrations of TP-16 for 12 hr. The mRNA levels of selected MDSC markers (Arg-1, Ptgs2, Il-4, and Il-10) were measured by qPCR. Data are normalized to β-actin expression levels (n=3)."
NCBI gene IDs:
"β-actin: 
Arg-1: 11846
Il-10: 16153
Ptgs2: 19225"
Figure legend: "D Percentage of GFP+ cells within T cell subpopulations 17 days after CD40LG editing of healthy male donor (HD; n=11) or patient (Pt; n=1) derived CD4+ T cells, measured by FACS analysis. Median ± IQR."
NCBI gene IDs:
"CD40LG: 959"
Figure legend: "G Percentage of TCRBV families detected by spectratyping. UT, sorted edited (GFP+), sorted non-edited (GFP-) CD4+ T cells derived from male HD (n=1) or patient (Pt; n=1) were analyzed at 17 days after CD40LG editing."
NCBI gene IDs:
"CD40LG: 959"
Figure legend: "B Percentage of NGFR+ cells or HDR at 17 days after CD40LG editing of male HD bulk edited, sorted NGFR+ or sorted NGFR- CD4+ T cells (n=7 for each group). Median ± IQR."
NCBI gene IDs:
"CD40LG: 959"
Figure legend: "I Dot plot depicting the ratio between expression of CD40LG edited mRNA (codon-usage optimized, CO) and CD40LG wild-type mRNA (WT) in cells edited with three different donor templates from Fig EV2H. Three independent experiments (n=3 EF1a.GFP, 1 IRES.NGFR, 3 IRES.GFP). Median ± IQR."
NCBI gene IDs:
"GFP: 
CD40LG: 959
EF1a: 1915
NGFR: 4804"
Figure legend: "J Time course of expression of WT-CD40LG mRNA or CO-CD40LG mRNA, measured as fold change (FC) on IPO8 housekeeping gene and normalized to T0. Sorted edited (+) and sorted unedited (-) CD4+ T cells were analyzed before and 3, 6, 12 hr after Actinomycin D treatment (n=1 for each group)."
NCBI gene IDs:
"IPO8: 
CD40LG: 959"
Figure legend: "D Bar plot showing percentage of EGFR+ T cells at 3 days after treatment with 5 nM or 1 nM of immunotoxin, antibody or toxin, measured by FACS Analysis. Friedman test with Dunn's multiple comparisons. P-values were adjusted with Bonferroni's correction to account for multiple comparisons (**p=0.0052 and **p=0.0024 for immunotoxin vs antibody and **p=0.0010 and **p=0.0024 for immunotoxin vs toxin, referring to EGFRmod1 and EGFRmod2 respectively). Different dose-conditions were used as a unified group for statistical analysis (n=10 for each group). Median ± IQR."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "B Percentage of HDR in HSPC subpopulations at 4 days after CD40LG editing, measured by molecular analysis (ddPCR). Cells were edited with donor template either comprising only the corrective cDNA (NR; n=4), or carrying the selector cassette IRES.NGFR (NGFR; n=5) and sorted according to surface markers: CD34-; CD34+CD133-CD90-; CD34+CD133+CD90-; CD34+CD133+CD90+. CD34+CD133+CD90+/CD90- and CD34+CD133-/CD34- conditions were used as unified groups for statistical analysis. The comparisons between the groups were performed with LME models accounting for the different subpopulations included in the analysis and with random effects defined to account for the same donor. In comparison about CD34+CD133+CD90+/CD90-, the percentages were used in square root scale to meet the assumption of normality of the residuals of the model. For CD34+CD133+CD90+/CD90-, **p= 0.0035, for CD34+CD133-/CD34- p=0.2051. Median ± IQR."
NCBI gene IDs:
"CD40LG: 959
NGFR: 4804"
Figure legend: "C Population composition in UT or bulk edited HSPC from (B) (n=8 for each group, except UT cells from NGFR group, n=7). Paired Wilcoxon's test, to account for the same donor, with p-values adjusted with Bonferroni's correction to account for multiple testing. Analysis was performed separately for NR and NGFR groups. Mean ± SEM."
NCBI gene IDs:
"NGFR: 4804"
Figure legend: "F TNP-KLH specific IgG in sera of transplanted mice collected at the times indicated in (A) (n=7 CPA 300, 13 NC for first boost and 4 CPA 300, 9 NC for second boost). Sera from vaccinated WT (n=5) and CD40lg-/- (n=5) mice were used as positive and negative controls, respectively. Two independent experiments. Comparisons of first boost data were performed with an LME model, accounting for multiple experiments, followed by appropriate post-hoc analysis (see Appendix Supplementary Statistical Methods). The reported statistical comparisons refer only to the overall difference among groups (****p&lt;0.0001 in all comparisons). Median ± IQR."
NCBI gene IDs:
"CD40lg: 21947"
Figure legend: "G Average area of PNA+ foci in splenic sections of experimental mice from (B), calculated as ratio between total PNA+ area and number of PNA+ foci/cells. Kruskal-Wallis test followed by post-hoc analysis with Dunn's test (n=9 CD40LG, 6 WT, 18 NC, 8 CPA300). P-values were adjusted with Bonferroni's correction to account for multiple comparisons (*p=0.0149, **p=0.0065 and ****p&lt;0.0001). Mean ± SEM."
NCBI gene IDs:
"CD40LG: 21947"
Figure legend: "H Detection of splenic TNP-KLH specific IgM (left) and IgG (right) -secreting cells by ELISPOT assay (n=7 NC, 4 CPA300, 3 WT, 2 CD40LG). Spots were counted by an ELISPOT Reader using a size range of 0.005-1 mm. Median ± IQR."
NCBI gene IDs:
"CD40LG: 21947"
Figure legend: "E TNP-KLH specific IgG concentration in sera of mice from (B) collected before (day 35; pre) and after (day 49; post) the first boost (n=13 NC, 19 CPA 300, 4 CPA 200, 5 ALS, 6 ANTI-CD4; three independent experiments) and at day 81 (pre) and 89 (post) for the second boost (n=4 NC, 4 CPA 300, 4 CPA 200, 5 ALS, 6 ANTI-CD4). Sera from vaccinated WT (n=5) and Cd40lg-/- (n=5) mice were used as positive and negative controls, respectively. For early challenge data, comparisons were performed with an LME model, accounting for multiple experiments, followed by appropriate post-hoc analysis (see Appendix Supplementary Statistical Methods). The reported statistical comparisons refer only to the overall difference among groups (***p=0.0002 and ****p&lt;0.0001 in all comparisons). CPA200 was not included in the analysis because n=4. WT group, not indicated in the figure, is significantly different from all groups (p&lt;0.0001 in all comparisons). Of note, regarding the comparisons between time-points, the groups CPA 300 and WT show a significant increase between pre and post values (p&lt;0.0001 for both), while all other groups show a significant decrease between pre and post values (p&lt;0.0001 for all except p=0.0022 for ANTI-CD4). Median ± IQR."
NCBI gene IDs:
"Cd40lg: 21947"
Figure legend: "H TNP-KLH specific IgG concentration in sera of mice from (F) collected before (day 36, pre) and after (day 50, post) the first boost (n=6 NC, 11 CPA 300, 11 CPA 200) and at day 212 (pre) and 219 (post) for the second boost (n=6 NC, 11 CPA 300, 9 CPA 200). Sera from vaccinated WT (n=11) and Cd40lg-/- (n=5) mice were used as positive and negative controls, respectively. Comparisons were performed with an LME model followed by an appropriate post-hoc analysis, separately for each challenge data (Appendix Supplementary Statistical Methods). Reported statistical comparisons refer to the overall difference among groups, for early challenge data (*p=0.0282 and **p=0.0037), while to differences between time-points within each group, for late challenge data (**p=0.0018 and ****p&lt;0.0001 in both comparisons). Of note, in the late challenge data, no significant overall differences were observed among the groups. Median ± IQR."
NCBI gene IDs:
"Cd40lg: 959"
Figure legend: "E Quantitation of P. murina rRNA in lung homogenate of mice from (A) transplanted with different ratios of WT HSPC cells (n=8 100% WT, 7 25% WT, 7 10% WT, 8 0% WT) and infected with the pathogen. Results are expressed in P.murina/HPRT RNA copies. Kruskal-Wallis test followed by post-hoc analysis with Dunn's test. P-values were adjusted with Bonferroni's correction to account for multiple comparisons. Only the groups 100% WT and 0% WT resulted to be significantly different (**p=0.0028). Median ± IQR."
NCBI gene IDs:
"HPRT: "
Figure legend: "H Quantitation of P. Murina rRNA in lung homogenate of mice adoptively transferred (n=9 CD40LG + T cells) or not (n=8 CD40LG) with in vivo primed CD4+ T cells in absence of conditioning and infected with the pathogen. Results are expressed in P.Murina/HPRT RNA copies. Mann-Whitney test. ***P-value of the comparison = 0.0003. Median ± IQR."
NCBI gene IDs:
"HPRT: 
CD40LG: 21947"
Figure legend: "(A) Netrin-1 (NTN1) gene expression profiling from 6 adults control brains using microarray data from the Allen Human Brain Atlas."
NCBI gene IDs:
"Netrin-1: 9423
NTN1: 9423"
Figure legend: "(B) Reporter gene expression in the midbrain (substantia nigra (SN) and ventral tegmental area (VTA)) of netrin-1+/lacZ+ adult (P60) mice incubated with S-Gal. (Scale bar, 2000 µm). Two coronal sections of the mice midbrain are shown from the more anterior is shown in the upper panel."
NCBI gene IDs:
"lacZ: 945006
netrin-1: 18208"
Figure legend: "(A) Representative images of dopamine neurons (TH-positive) in the substantia nigra (SN) (left panel) and in the SN and striatum (right panel top, and bottom respectively) by immunofluorescence, six weeks after the intranigral injection of control (CTL) (AAV6-GFP) or Cre (AAV6-GFP Cre) adenovirus vectors in Netrin-1fl/fl mice (left panel: green-GFP, red-TH; right panel: Cy-5-TH) (Scale bar, 1000 μm). Filled arrows indicate SN TH dopamine neurons."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Netrin-1: 18208"
Figure legend: "(B) Representative images of TH and netrin-1 stainings by immunofluorescence (scale bar, 2000 μm) six weeks after the intranigral injection of CTL adenovirus vector (left panel) or Cre adenovirus vector (right panel), in the midbrain (substantia nigra (SN) and ventral tegmental area (VTA) of Netrin-1fl/fl mice."
NCBI gene IDs:
"Cre: 2777477
Netrin-1: 18208"
Figure legend: "(C) Quantification of fluorescence intensity of TH-positive neurons, in the substantia nigra (SN) (left), and fibres, in the striatum (right), six weeks after adenovirus vector injection, in Netrin-1fl/fl mice. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t-test. *P &lt; 0.05; **P&lt; 0.01. N=3 each group."
NCBI gene IDs:
"Netrin-1: 18208"
Figure legend: "(D) Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labelling (TUNEL) reactivity in the SN after Cre and control adenovirus vector injection, in Netrin-1fl/fl mice (Scale bar, 50μm). The apoptotic index is expressed as a percentage of TUNEL positive neurons out of the total number of TH-positive neurons. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t-test. **P&lt; 0.01. N=3 each group."
NCBI gene IDs:
"Cre: 2777477
Netrin-1: 18208"
Figure legend: "(F) Immunoblot of SN lysates from netrin-1 wild type (WT) and netrin-1 conditional KO mice (left upper panel). Densitometry band quantification of cleaved receptor over full length receptor ratio (lower right panel). We randomly selected 2 mice from each group for immunoblot analysis. N=3 independent experiments. Bars and error bars represent the mean ± SEM. Statistical significance was determined by an unpaired t-test. *P &lt; 0.05; P**&lt; 0.01."
NCBI gene IDs:
"netrin-1: 18208"
Figure legend: "(A) Representative images of TH+ dopamine neurons and fibers staining by immunofluorescence, in the SN and the striatum (Str) 6 weeks after CTL (AAV6-GFP); Cre (AAV6-GFP Cre); sh DCC (AAV6-GFP Cre + Sh-DCC) and shUNC5B (AAV6 GFP Cre + Sh-UNC5B) injection into the SN of Netrin-1fl/fl mice. (Scale bar, 2000 µm). (B) Quantification of TH fluorescent intensity in the SN (upper bar graph) and striatum (Str) of Netrin-1fl/fl mice (lower bar graph). Data are shown as mean + SEM. Statistical significance was determined by an unpaired t-test. N = 3 each group. Tukey post hoc analysis after one-way ANOVA *P &lt; 0.05, **P &lt;0.01, N.S., not significant."
NCBI gene IDs:
"GFP: 
Cre: 2777477
DCC: 13176
Netrin-1: 18208
UNC5B: 107449"
Figure legend: "(C) DCC depletion mitigates netrin-1 depletion-induced apoptosis in Netrin-1fl/fl mice. TH-positive cell loss was assessed by TUNEL assay in the SN. Upper panel, TUNEL (red) and TH (Cy5-white) (Scale bar, 50 µm). Apoptotic index (bar graph, bottom panel) expressed as a percentage of TUNEL positive neurons out of the total number of TH-positive neurons. N = 3 each group. Bars and error bars represent the mean ± SEM. Statistical significance was determined using a one-way ANOVA followed by post hoc Tukey test for multiple group comparison. *P &lt; 0.05; P**&lt; 0.01."
NCBI gene IDs:
"DCC: 13176
Netrin-1: 18208
netrin-1: 18208"
Figure legend: "(F) TH staining by immunohistochemistry (Scale bar, 2000µm) in the SN (left) and striatum (right) of WT and SNCA Tg mice, 6 days after treatment."
NCBI gene IDs:
"SNCA: 6622"
Figure legend: "(A) Netrin-1 gene expression profiling by array of substantiae nigrae from PD and non-PD (Control) patients using the GEO dataset GSE7621 that has a total of 25 samples (n=9 control and n=16 PD cases). Unpaired t-test, ***P &lt; 0.0005, Mean ± SD are shown."
NCBI gene IDs:
"Netrin-1: 9423"
Figure legend: "(D)  EI24  knockout promotes the activation of IRE1, but not  PERK or ATF6. Control (sgGFP) and EI24 knockout (sgEI24) HeLa cells were treated with 4 μM  TM for the indicated  times. Protein expression  was analyzed by immunoblotting."
NCBI gene IDs:
"GFP: 
EI24: 9538"
Figure legend: "(G) Expression of  Flag-EI24 in EI24 knockout cells inhibits IRE1 activity. Flag-Metap2 served as the control. Cells transfected with the indicated cDNAs were treated with or without 4 μM TM for 2 hr.  Protein expression  was analyzed by  immunoblotting."
NCBI gene IDs:
"EI24: 9538"
Figure legend: "(B)  EI24 persistently  localizes on the ER regardless of the presence or absence of ER stress. HeLa cells stably expressing  Flag-EI24 were treated with or without 4 μM  tunicamycin  (TM) for 2 hr. Colocalization  of EI24 with ER was analyzed. KDEL-mCherry, ER marker. Scale bar, 10 μm."
NCBI gene IDs:
"Flag: 
EI24: 9538"
Figure legend: "(D) The C-terminus of EI24 is required for IRE1 binding. HeLa cells transfected with the indicated EI24 mutants were treated as in (B), after which protein-protein interactions  were analyzed by immunoprecipitation (IP). The star represents IgG light chains. (E) The C-terminus of  EI24 binds  to IRE1 in a persistent fashion. HeLa cells transfected with the indicated EI24 domains were treated as in (B). Protein-protein interactions  were analyzed by  IP. The star represents IgG light chains. ("
NCBI gene IDs:
"EI24: 9538"
Figure legend: "(I) EI24  overexpression cannot prevent the activation of the phospho-mimicking (3SE)  IRE1 mutant. Wild-type and IRE1 3SE knock-in HeLa  cells were transfected with empty vector or Flag-EI24. Transfected cells were treated as in (B). Protein expressions were analyzed by immunoblotting."
NCBI gene IDs:
"Flag: 
EI24: 9538
IRE1: 2081"
Figure legend: "(L) Expression of full-length  EI24 or the △TM34 or △C mutant in EI24  knockout  HeLa  cells for comparison of their functions. HeLa cells transfected with the indicated EI24 mutants were treated as in (B). Protein expressions were analyzed by immunoblotting."
NCBI gene IDs:
"EI24: 9538"
Figure legend: "(B, C)  EI24 knockout causes resting-state  ER calcium  reduction (B) and cytosolic calcium elevation (C) under ER  stress. Control (sgGFP) and EI24 knockout (sgEI24)  HeLa cells were first perfused in  TM-free buffer, and switched to the buffer containing 4 μM  TM to induce ER stress where indicated. Resting state calcium levels in the  ER (B) and cytosol (C) with or without  TM are displayed in columns. Data information: N = 20 cells for one replicate. Data are mean ± SEM (n = 3 technical replicates). ns, no significant difference; ***P &lt; 0.001; two-tailed Student's t-test."
NCBI gene IDs:
"GFP: 
EI24: 9538"
Figure legend: "(G) Knockdown of IP3R1 prevents  calcium mobilization  from the  ER to the  cytosol. The measurements were similarly performed as in (E), and were from the same batch of cells. Data information: N = 20 cells for one replicate. Data are mean ± SEM (n = 3 technical replicates). ns, no significant difference; ***P &lt; 0.001; two-tailed Student's t-test."
NCBI gene IDs:
"IP3R1: 3708"
Figure legend: "(I) Wild-type  EI24, but not the TM34- or C-terminus-deleted mutant, preserves  ER calcium  stores in EI24 knockout cells. The measurements were similarly performed as in (E), and were from the same batch of cells. Data information: N = 20 cells for one replicate. Data are mean ± SEM (n = 3 technical replicates). ns, no significant difference; ***P &lt; 0.001; two-tailed Student's t-test."
NCBI gene IDs:
"EI24: 9538"