EMBO/SourceData huggingface dataset:
Figure legend: "E and F, Immunohistochemical staining  of ARG1  and ASS1  in fetal lungs  of WT and Src-1/-2 dKO mice  at 18.5 dpc. PBS  was used instead of the primary antibody as a negative control (NC). Scale bars: 50 μm in E and F."
NCBI gene IDs:
"Src-1: 17977"
Figure legend: "A and C, mRNA expression  of Arg1  and Ass1  in the lungs  of WT fetuses  at gestational ages  from 15.5 dpc to labor. n=6 biological replicates for each gestational age  in panel A; n=6 biological replicates for 15.5dpc, 17.5dpc, and labor group, n=5 biological replicates for 16.5dpc and 18.5dpc group in panel C. Kruskal-Wallis test followed by Dunn's multiple comparison test was used to analyze the data. The data shown are the mean ± s.e.m. **P&lt;0.01, ****P&lt;0.0001."
NCBI gene IDs:
"Arg1: 11846
Ass1: 11898"
Figure legend: "D and E, Histograms showing the content of arginine, ornithine, ADMA  and the ratio of arginine  to ADMA  in the fetal lungs  of WT mice  and Src-1/-2 dKO mice  at 18.5 dpc. Mann Whitney test was used to analyze the data in panel D, and unpaired t test was used to analyze the data in panel E. n=5 biological replicates for each group, and the data are shown as the mean ± s.e.m. The central band indicates the median, the boxes indicate the upper quartile and lower quartile, and the whiskers indicate maximum value and minimum value of the data. *P&lt;0.05, **P&lt;0.01, compared with the WT fetal lungs."
NCBI gene IDs:
"Src-1: 17977"
Figure legend: "B, Representative immunoblots  of ARG1 expression  in the fetal lungs  from the AAV-shArg1-injected group and the AAV-2/9-injected group. Densitometric  quantitation of scans of immunoblots  (normalized to tubulin) is also shown as a bar graph. Unpaired t test was used to analyze the data and n=3 biological replicates for each group. The data are shown as the mean ± s.e.m. **P&lt;0.01 compared with the AAV-2/9-injected group."
NCBI gene IDs:
"Arg1: 11846"
Figure legend: "C, Representative immunoblots  and densitometric  quantitation of Ki67, caspase3  precursor (pro-caspase3) and cleaved caspase3 expression  in the fetal lungs  from the AAV-shArg1-injected group and AAV-2/9-injected group. Unpaired t test was used to analyze the data and n=3 biological replicates for each group. The data are shown as the mean ± s.e.m. **P&lt;0.01 compared with the AAV-2/9-injected group; n.s indicates non-significance."
NCBI gene IDs:
"Arg1: 11846"
Figure legend: "E, Multiple immunofluorescence staining  and densitometric  quantitation of TUNEL  with PDPN  antibody (type I alveolar epithelial cells  marker) on sections of fetal lungs  from AAV-shArg1-injected mice  vs. AAV-2/9-injected mice. Blue: DAPI  (nuclei); Green: PDPN  (type I cells); Red: TUNEL  positive. Unpaired t test was used to analyze the quantitation data and n=3 biological replicates generated from the mean intensity of 3 randomly selected fields of view for each group. The data are shown as the mean ± s.e.m. ***P&lt;0.001 compared with the AAV-2/9-injected group. F, Multiple immunofluorescence staining  and densitometric  quantitation of TUNEL  with LPCAT1  antibody (type II alveolar epithelial cells  marker) on sections of fetal lungs  from AAV-shArg1-injected mice  vs. AAV-2/9-injected mice. Blue: DAPI  (nuclei); Green: LPCAT1  (type II cells); Red: TUNEL  positive. Unpaired t test was used to analyze the quantitation data and n=3 biological replicates generated from the mean intensity of 3 randomly selected fields of view for each group. The data are shown as the mean ± s.e.m. ***P&lt;0.001 compared with the AAV-2/9-injected group. Data information: Scale bars in E and F: 50 μm."
NCBI gene IDs:
"Arg1: 11846"
Figure legend: "C, Representative western blots  and quantitative analysis of CAP  expression  in the myometrium  of pregnant mice injected intraplacentally  with AAV-shArg1 or AAV  control viruses. Mann Whitney test was used to analyze CX43  and PTGFR  data and unpaired t test was used to analyze COX2  and OXTR data. n=4 biological replicates for CX43, COX2  and PTGFR  groups, and n=5 biological replicates for OXTR  group. The data are shown as the mean ± s.e.m. *P &lt; 0.05."
NCBI gene IDs:
"Arg1: 11846"
Figure legend: "D, Representative  western blots  and quantitative analysis of   NF-κB  p-p65   (phospho  S536) and NF-κB p65 expression  in the myometrium  of pregnant mice injected intraplacentally  with AAV-shArg1 or AAV  control viruses. Unpaired t test was used and n=4 biological replicates for each group. The data are shown as the mean ± s.e.m. **P &lt; 0.01."
NCBI gene IDs:
"Arg1: 11846"
Figure legend: "Chromatin immunoprecipitation  (ChIP) was performed with WT or Src-1/-2 dKO fetal lung  tissues using antibodies against GR  (B) Enrichment of these transcription factors at the GRE   or  C/EBPβ binding  sites within the Arg1  promoter were determined by quantitative PCR. Unpaired t test was used for panel B and n=6 biological replicates for each group. The data are presented as the mean ± s.e.m. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001."
NCBI gene IDs:
"Arg1: 11846
Src-1: 17977"
Figure legend: "Chromatin immunoprecipitation (ChIP) was performed with WT or  Src-1/-2 dKO fetal lung  tissues using antibodies against SRC-1  (C), SRC-2 (D), or C/EBPβ (E). Enrichment of these transcription factors at the GRE   or C/EBPβ binding  sites within the Arg1  promoter were determined by quantitative PCR. Mann Whitney test was used for Panel C-E. n=6 biological replicates for each group. The data are presented as the mean ± s.e.m. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001. F, Quantitative PCR results for GRE   and C/EBP binding  site using \"input\" (positive control) DNA without being immunoprecipitated  with any specific antibody. n=6 biological replicates for each group. The data are presented as the mean ± s.e.m."
NCBI gene IDs:
"Arg1: 11846
Src-1: 17977"
Figure legend: "p53 ChIPseq in MEFs of the indicated genotype treated with or without 10 µM nutlin-3a (Nutlin) for 16 hours. Shown are 2 kb regions surrounding the summit of the 468 p53 binding peaks called in Nutlin-treated p53+/+, but not p53-/- or p53EE/EE MEFs. For p53EE/EE MEFs three independent replicates are shown."
NCBI gene IDs:
"p53: 22059"
Figure legend: "RNAseq was performed with MEFs of the indicated genotype untreated or treated with 10 µM Nutlin for 16 hours. Shown are all Nutlin-regulated genes from the MSigDB gene set Hallmarks_P53_Pathway with a mean log2FC≥1 in p53+/+ cells. Shown are the z-transformed RNA expression values (FPKM)."
NCBI gene IDs:
"p53: 22059"
Figure legend: "RNAseq data were subjected to gene set enrichment analysis (GSEA). Shown are enrichment plots for the indicated set of p53 downstream genes in pairwise comparisons of Nutlin-treated MEFs with the indicated p53 genotypes."
NCBI gene IDs:
"p53: 22059"
Figure legend: "Reverse transcription quantitative PCR (RTqPCR) analysis of p53 target genes in MEFs treated for 24 hours with 1 µg/ml doxorubicin (Doxo). Shown are expression values normalized to β-actin as mean ± SD (n=6)."
NCBI gene IDs:
"β-actin: "
Figure legend: "MEFs were immortalized with the adenoviral oncogene E1A.12S (E1A MEF) and treated with 0.4 µg/ml Doxo for 17 hours. Apoptosis (Annexin V) was quantified by flow cytometry. +/+ and -/-: n=3; EE/EE: n=6. Western blots show expression of E1A and β-actin as loading control."
NCBI gene IDs:
"E1A: 
E1A.12S: "
Figure legend: "mRNA expression analysis (RTqPCR) of p53 target genes in thymocytes 6 hours after 6 Gy irradiation. Shown are expression values normalized to β-actin."
NCBI gene IDs:
"β-actin: "
Figure legend: "Observed and expected genotype distribution of newborn offspring from mating Mdm2+/-Trp53EE/+ mice (total number of pups n=185; contingency test P=0.0029). Observed and expected genotype distribution of newborn offspring from mating Mdm2+/-Trp53+/- mice (total number of pups n=435; contingency test P=0.912)."
NCBI gene IDs:
"Mdm2: 17246
Trp53: 22059"
Figure legend: "Representative picture of an E9.5 Mdm2-/-Trp53EE/EE embryo (right) displays characteristic phenotypic abnormalities compared to an Mdm2+/-;Trp53EE/EE embryo (left)."
NCBI gene IDs:
"Mdm2: 17246
Trp53: 22059"
Figure legend: "IHC for p53 and cleaved caspase-3 shows strong accumulation of p53EE protein and massive apoptosis in Mdm2-/-Trp53EE/EE compared to Mdm2+/-;Trp53EE/EE control embryos."
NCBI gene IDs:
"Mdm2: 17246
Trp53: 22059"
Figure legend: "Mdm2-/-Trp53-/- (double knock-out, DKO) MEFs were transduced with pInducer20-p53EE lentivirus to enable tetracyclin (Tet)-inducible expression of mouse p53EE. Induction of p53EE sensitized cells to apoptosis induced by 24 hour treatment with 1 µg/ml doxorubicin (Doxo) as detected by flow cytometry for Annexin V."
NCBI gene IDs:
"Mdm2: 17246
Trp53: 22059
p53: 22059"
Figure legend: "mRNA expression of p53 target genes was measured in cells from (A) relative to β-actin by RTqPCR following treatment with Tet ± 1 µg/ml Doxo. Expression of the pro-apoptotic p53 target genes Bbc3 (Puma) and Bax is not significantly induced. n=6."
NCBI gene IDs:
"β-actin: 
Bax: 12028
Bbc3: 170770
Puma: 170770"
Figure legend: "Western blot of E1A-MEFs with indicated genotypes treated with 10 µM Nutlin ± 0.4 µg/ml Doxo for 18 hours reveals induction of apoptosis (cCasp-3, cleaved caspase-3; cParp, cleaved Parp) in double-treated p53EE/EE MEFs in the absence of p53 target gene (p21, Mdm2) activation. *, 10 hours 0.4 µg/ml Doxo; **, 10 hours 0.2 µg/ml Doxo."
NCBI gene IDs:
"E1A: 
p53: 22059"
Figure legend: "mRNA expression of p53 target genes was measured in E1A MEFs of indicated genotype relative to β-actin by RTqPCR following combined treatment with 10 µM Nutlin and 1 µg/ml Doxo. n=12."
NCBI gene IDs:
"E1A: 
β-actin: "
Figure legend: "Western blot of p53EE/EE E1A-MEFs with indicated genotypes treated for 18 hours with 10 µM Nutlin, 0.4 µg/ml Doxo and 30-100-500 nM Ganetespib as indicated. Comb, combined treatment with 3 drugs for 18 hours. Pre, pre-treatment with ganetespib for 24 hours followed by combined treatment with Nutlin and Doxorubicin for 18 hours."
NCBI gene IDs:
"E1A: 
p53: 22059"
Figure legend: "Cell death measured by flow cytometry using propidium iodide exclusion in human lung adenocarcinoma H1299 cells with tet-inducible expression of human p53EE. Cells with overexpression of human p53EE mutant display a trend of increased cell death after treatment with 0.5 µg/ml Doxo."
NCBI gene IDs:
"p53: 7157"
Figure legend: "Cell death measured by flow cytometry using propidium iodide exclusion in human lung adenocarcinoma H1299 cells with tet-inducible expression of human p53EE. Cells with overexpression of human p53EE mutant display a trend of increased cell death after treatment with 0.5 µg/ml Doxo. This effect is enhanced by addition of Nutlin and blocked by the pan-caspase inhibitor QVDOph, but not the ferroptosis inhibitor Ferrostatin-1 (Ferr) as control. (A) n=3; (B) n=2."
NCBI gene IDs:
"p53: 7157"
Figure legend: "mRNA expression analysis (RTqPCR) of p53 target genes following treatment with 0.5 µg/ml Doxo ± 10 µM Nutlin. KO, tet-induced H1299-pInd cells; EE, tet-induced H1299-pInd-p53EE cells; WT, H460 cells. mRNA expression was normalized to β-actin; n=3."
NCBI gene IDs:
"β-actin: 
p53: 7157"
Figure legend: "Mitochondrial membrane potential of control and p53EE expressing H1299 cells in the absence and presence of increasing concentrations of BID BH3 peptide. Treatment with the mitochondrial depolarizer CCCP is shown as a positive control; n=3."
NCBI gene IDs:
"p53: 7157"
Figure legend: "Apoptosis measured by flow cytometry using Annexin V in H1299 cells with tet-inducible expression of the indicated human p53 mutants; n=3."
NCBI gene IDs:
"p53: 7157"
Figure legend: "mRNA expression analysis (RTqPCR) of p53 target genes normalized to β-actin; n=3."
NCBI gene IDs:
"β-actin: "
Figure legend: "Kaplan-Meier survival plots for Eµ-Myc transgenic mice with different p53 genotype. Kaplan-Meier survival plots for wild-type recipient mice transplanted with AML1/ETO9a + NrasG12D-transduced fetal liver cells (5 donors/genotype)."
NCBI gene IDs:
"Myc: 
Nras: 18176
AML1: 861
ETO9a: 862
p53: 22059"
Figure legend: "Enrichment of p53 shRNA-expressing Eµ-Myc;p53EE lymphoma cells under MAF treatment. Non-silencing control shRNA served as control. n=3."
NCBI gene IDs:
"Myc: 
p53: 22059"
Figure legend: "mRNA expression of p53 target genes was measured relative to β-actin by RTqPCR following treatment of Eµ-Myc lymphoma cells of indicated genotype with 3 µg/ml MAF. n≥3."
NCBI gene IDs:
"β-actin: 
Myc: "
Figure legend: "Kaplan-Meier survival plots for cyclophosphamide-treated versus untreated control mice transplanted with Eµ-Myc lymphoma cells of indicated genotype at day 0. Treatment started ~10 days later when peripheral lymph nodes became palpable. The time course of the experiment is illustrated in a scheme."
NCBI gene IDs:
"Myc: "
Figure legend: "Residual disease in the spleen of mice from (D) was quantified 24 hours and 7 days after therapy using a quantitative PCR assays with primers specific to the Eµ-Myc transgene (present only in lymphoma cells). Shown is the copy number of the Eµ-Myc transgene relative to a control locus present in all cells. Untreated Eµ-Myc mice (Myc tg) were used as positive control. Non-transgenic mice (no Myc tg) served as negative controls to define the detection limit. Significance was tested by t-test (two-sided, unpaired)."
NCBI gene IDs:
"Myc: "
Figure legend: "Representative bioluminescence imaging (BLI) pictures of mice transplanted with AE9+Nras AML cells. Day 0 indicates the start of combination therapy with cytarabine (AraC) + doxorubicin (Doxo)."
NCBI gene IDs:
"AE9: 
Nras: 18176"
Figure legend: "Kaplan-Meier survival plots for animals from (G). Statistical significance of differences calculated by Log-rank (Mantel-Cox) test between treated Eµ-Myc;p53EE/EE AML and all other groups is indicated in the table. C, untreated control; T, therapy (AraC+Doxo)."
NCBI gene IDs:
"Myc: 
p53: 22059"
Figure legend: "Antibacterial competition assay. E. coli K12 recipient cells (W3110 gfp+, kanR) were mixed with the indicated EAEC attacker cells (1:4 ratio): WT, ∆T6SS-1 and ∆tle1-tli1 carrying pBAD18 and pBAD33 vectors, or producing the indicated proteins, and spotted on SIM 0.02 % arabinose agar plates for 4-h at 37°C. The image of a representative bacterial spot is shown. The number of recovered prey cells is indicated in the upper graph (in log of colony-forming units (cfu)). The black, dark grey and light grey circles indicate values obtained from three different spots, and the average is indicated by the bar. Western blot analysis of the production of Tle1V and Tle1∆1-26 V is shown in the inset. The experiment was performed in triplicate and a representative result is shown."
NCBI gene IDs:
"T6SS-1: 
tle1: 
tli1: "
Figure legend: "(C) Timelapse of ATP sensor FRET efficiency in the apGalts&gt;PtcRNAi wing pouch after 10 μM antimycin A (ant.A) addition. Red dashed line indicates the position of the AP boundary."
NCBI gene IDs:
"ap: 35509
Ptc: 35851"
Figure legend: "(D, F) Mean FRET efficiency analyzed over time in the (D) dorsal anterior (DA) and ventral anterior (VA) sub-compartments or the (F) dorsal anterior (DA) and dorsal posterior (DP) sub-compartments of apGalts&gt;PtcRNAi wing discs. Shaded regions indicate SD; dots represent the mean per timepoint; solid lines illustrate a fit of the mean data."
NCBI gene IDs:
"ap: 35509
Ptc: 35851"
Figure legend: "(I) Timelapse of ATP sensor FRET efficiency in apGalts &gt; CiDN wing discs after 10 μM antimycin A addition."
NCBI gene IDs:
"ap: 35509
Ci: 43767"
Figure legend: "(J) Mean FRET efficiency measured over time in the dorsal and ventral compartments in apGalts &gt; CiDN wings. Shaded regions indicate SD; dots represent the average per timepoint; solid lines illustrate a fit of the mean data."
NCBI gene IDs:
"ap: 35509
Ci: 43767"
Figure legend: "Gene expression of NOTCH3, HES1, HEY1, HEYL and the 17 recently identified targets were established by a real-time PCR panel assay in skeletal muscle of controls (n = 6), proband (II:1), parents (mother I:1, father I:2) and CADASIL patients (n = 3). Graph shows gene expression fold changes relative to controls and normalized on GAPDH (reference gene), expressed as mean of two experiments ± SEM. Among the 17 novel assayed target genes, RCAN2, ANGPT4, HP and SORBS were not detected in any sample and were therefore not reported. Green and red lines indicate 2.0 and 0.5 fold changes, respectively. Statistical significances and P-values are reported in Supplementary Table S1."
NCBI gene IDs:
"GAPDH: 
ANGPT4: 51378
HES1: 3280
HEY1: 23462
HEYL: 26508
HP: 3240
NOTCH3: 4854
RCAN2: 10231
SORBS: 10174///8470///10580"
Figure legend: "(C) Whole-mount immunohistochemistry with anti-neurofilaments antibody for visualization of peripheral nerves. In crmp2-/- embryos, the growth of ophthalmic branch of the trigeminal nerve is increased and axons are defasciculated (first and second row, arrowheads). Similarly, we detected increased growth and branching of lateral branches of spinal nerves in crmp2-/- embryos (third row, arrowheads). Trigeminal nerve (V) and its branches (Op - ophthalmic, Mx - maxillar, Md - mandibular) are indicated. Scale bars: 500 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(D, E) Quantification of areas innervated by ophthalmic branches of trigeminal ganglion and spinal nerve, normalized to WT littermates (each dot depicts one embryo). Area of ophthalmic branch in crmp2-/- was increased by 66% (p&lt;0.05, n=10, 3 litters). For spinal nerves, total area in knockouts was increased by 45% (p&lt;0.05, WT n=8, crmp2-/- n=7, 3 litters). Mean ± SD, * p&lt;0.05, t-test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(F) Growth of motor neurons from E11.5 - E12.5 WT and crmp2-/- spinal cord explants in microfluidic chambers. Excerpts from 14 hour time-lapse imaging are shown. Upon application of Sema3A into distal compartment, WT axons tend to stop or slow down their growth unlike crmp2-/- axons. Purple lines highlight the growth path of individual axons in a distal chamber. Scale bars: 100 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(G) Quantification of the fraction of growing (&gt;50 μm) axons in one imaging field (WT: control 76.8±5.7%, Sema3A 57.7±10.6%, p&lt;0.001, crmp2-/-: control 75.4±10.4%, Sema3A 75.4±6.7%, p=0.99), n=3 experiments per genotype, 7-8 explants per condition. Mean ± SD. *** p&lt;0.001, 2-way ANOVA with Bonferroni's multiple comparisons test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(A) CRMP2 deficiency leads to callosal hypoplasia. Shortening of corpus callosum (arrowheads) is apparent in both sagittal (first row) and coronal sections (second row) of adult brains. Scale bars: 1 mm."
NCBI gene IDs:
"CRMP2: 12934"
Figure legend: "(B) Quantification of callosal length in 30-days old mice (P30, n=3, crmp2-/- is 80.8±5% of WT, p=0.003) and adult mice (n=3, crmp2-/- is 80.5±3% of WT, p=0.002), mean ± SD, * p&lt;0.05, t-test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(C) Labeling of adult corpus callosum with anti-neurofilaments antibody in sagittal sections. Outline depicts missing posterior part of the tract in crmp2-/- mice. Caudal part of the corpus callosum in WTs is located dorsally above the habenular commissure (hbc), while in crmp2-/- mice callosum terminates rostrally before reaching the hbc (arrows). PC indicates posterior commissure. Scale bars: 500 µm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(D) The growth of GFP-labeled callosal axons in the contralateral cortex at P6 (embryos were electroporated at E15.5). Note the disorganized paths of crmp2-/- axons. Scale bars: 200 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(F) Quantification of tortuosity of axons (WT n=6 pups, crmp2-/- n=3 pups) upon their exit from the callosal tract (WT 1.016±0.003 vs. crmp2-/- 1.044±0.012, p&lt;0.001), mean ± SD, ***p&lt;0.001, t-test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(G) DiI-labeled callosal axons from P9 oblique brain sections and their reconstruction in Neurolucida 360 (WT n=6, crmp2-/- n=9). Scale bars: 200 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(H) Polar histograms of callosal axons reconstructed Note the broader range of axon growth angles in crmp2-/- mice."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(I) Left: schematic representation of polar histogram analysis by clustering the traced axons (WT n=6 pups, crmp2-/- n=9 pups) into 3 groups based on the growth angles. Right: proportion of axons growing in selected clusters (WT, 0°-±20°: 67.3±10.3%; ±20°-±40°: 21.2±5.3%; ±40°-±60°: 6.22±3.2%; crmp2-/-, 58.5±5%, p=0.047, 27.7±4.3%, p=0.022, 8.64±2.25%, p=0.11), mean ± SD, *p&lt;0.05, t-test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(A, B) (A) Coronal sections of P14 brains stained for calbindin and VgluT1. Infrapyramidal bundle (IPB) progresses into hippocampal CA3 region in both WT and crmp2-/- mice. Nuclei are counterstained with Hoechst 33342. (B) Details from depicted regions in (A), arrowheads show the course of IPB. Scale bars: 100 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(C, D) (C) Staining for calbindin shows unpruned infrapyramidal bundle in adult crmp2-/- mice. No VGluT2 signal in hippocampal CA3 region is present in WT and crmp2-/- suggesting mature synapses. Scale bars: 500 μm. (D) Details from depicted regions in (C), arrowheads show the course of IPB. Scale bars: 100 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(E, F) Staining for calbindin and VgluT1 shows mature synapses in the main bundle in both genotypes and in the IPB in crmp2-/- mice (arrowheads). Scale bars: 100 μm."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(G) Quantification of IPB index (length of the IPB vs. main bundle, WT 0.43±0.05, crmp2-/- 0.78±0.05, p&lt;0.001) counted from adult coronal sections (WT n=4 mice, crmp2-/- n=5 mice). Mean ± SD, ***p&lt;0.001, t-test."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(C) Quantification of VP (visual pruning) index (fluorescence intensity of pyramidal axons after vs. before the branch point). In WT animals after the pruning period, only a minor part of axons descends towards the pyramidal tract (WT: P9 0.65±0.09, adults 0.17±0.04, p=0.006). However, in crmp2-/- mice, corticospinal axons are still largely present, and their VP indexes are not significantly different between adult and P9 stages (crmp2-/-: P9 0.54±0.18, adults 0.62±0.27, p=0.24). Mean ± SD, **p&lt;0.01, 2-way ANOVA with Bonferroni's multiple comparisons test. P9: n=3 pups/genotype, adults: n=5 mice/genotype)"
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(A) Time-lapse imaging of DIV7 cultured hippocampal neurons before and after Semaphorin stimulation. Upper panel: Axon growth without Semaphorin stimulation. Middle panel: stimulation with Sema3A (0 hours) (1nM, n=669 axons for WT, 761 axons for knockout) causes retraction of both WT and crmp2-/- neurons. Lower panel: stimulation with Sema3F (5 nM, n=602 axons for WT, 955 axons for knockout) causes axon retraction in WT, but not in crmp2-/- neurons. Red triangles depict growing axons, yellow retracting axons and blue asterisks indicate steady non-growing axons. Scale bars: 100 μm (whole image field) and 50 μm (magnified)."
NCBI gene IDs:
"crmp2: 12934"
Figure legend: "(C) Quantification of retracting axons (number of retracting vs. steady axons, 3 experiments). WT: control 13.4±5%, Sema3A 31.2±6% (p&lt;0.001), Sema3F 36.9±8.7% (p&lt;0.001); crmp2-/-: control 19.8±1.8%, Sema3A 28.5±4.4% (p&lt;0.05), Sema3F 22.4±4.1% (p&gt;0.99), mean ± SD are shown. ***p&lt;0.001, *p&lt;0.05, 2-way ANOVA with Bonferroni's multiple comparisons test."
NCBI gene IDs:
"crmp2: 12934"