EMBO/SourceData huggingface dataset:
Figure legend: "K. Compilation of representative compressed Z-stack confocal images of FITC-dextran-labeled CNV and IB4-stained laser impact area from LysM-Cre/Nrp1+/+ and LysM-Cre/Nrp1fl/fl mice at D14. Scale bar: 20μm. L-N. Quantification of area of FITC-dextran-labeled CNV (L), isolectin B4 (IB4 )-stained laser impact area (M) and the ratio of FITC/IB4 per laser-burn (N) relative to LysM-Cre/Nrp1+/+ at D14; n = 23 burns (LysM-Cre/Nrp1+/+), n = 27 burns ( LysM-Cre/Nrp1fl/fl)."
NCBI gene IDs:
"Cre: 2777477
LysM: 4069
Nrp1: 8829"
Figure legend: "A-D. mRNA expression of inflammation markers relative to LysM-Cre/Nrp1+/+ in mouse RPE-choroid-sclera complexes at D3 for Il1b (A); n = 10 (LysM-Cre/Nrp1+/+ and LysM-Cre/Nrp1fl/fl), Il6 (B); n = 7 (LysM-Cre/Nrp1+/+ and LysM-Cre/Nrp1fl/fl), Vegfa (C); n = 10 (LysM-Cre/Nrp1+/+ and LysM-Cre/Nrp1fl/fl), Tnf (D); n = 5 (LysM-Cre/Nrp1+/+), n = 10 (LysM-Cre/Nrp1fl/fl)."
NCBI gene IDs:
"Cre: 2777477
Il1b: 3553
Il6: 3569
LysM: 4069
Nrp1: 8829
Tnf: 7124
Vegfa: 7422"
Figure legend: "K, L. mRNA expression relative to LysM-Cre/Nrp1+/+ of inflammation markers in mouse BMDMs for Il1b (K); n = 4 (LysM-Cre/Nrp1+/+), n = 5 (LysM-Cre/Nrp1fl/fl) and Il6 (L); n = 3 (LysM-Cre/Nrp1+/+), n = 2 (LysM-Cre/Nrp1fl/fl)"
NCBI gene IDs:
"Cre: 2777477
Il1b: 16176
Il6: 16193
LysM: 4069
Nrp1: 8829"
Figure legend: "P. Heatmap (left) and enrichment plot (right) of GO Angiogenesis gene set enrichment analysis (GSEA) of wildtype and LysM-Cre/Nrp1fl/fl peritoneal macrophages; n = 2. NES, normalized enrichment score; FDR, false discovery rate."
NCBI gene IDs:
"Cre: 2777477
LysMbutton type=\"button\" class=\"btn btn-success btn-xs\" ng-click=\"confirmPretag()\" ng-show=\"!showA: 4069
Nrp1: 8829"
Figure legend: "J-L. Quantification of area of FITC-dextran-labeled CNV (J), isolectin B4 (IB4)-stained laser impact area (K) and the ratio of FITC/IB4 per laser-burn (L) relative to LysM-Cre/Nrp1+/+ + Vehicle in Vehicle and NRP1-derived trap treated LysM-Cre/Nrp1+/+ and LysM-Cre/Nrp1fl/fl mice at D14; n = 16 burns (LysM-Cre/Nrp1+/+ + Vehicle), n = 16 burns (LysM-Cre/Nrp1+/+ + Trap), n = 13 burns (LysM-null/Nrp1fl/fl + Vehicle) , n = 19 burns (LysM-Cre/Nrp1fl/fl + Trap)."
NCBI gene IDs:
"Cre: 2777477
LysM: 4069
Nrp1: 8829"
Figure legend: "(C) Representative images of P90 SC cross sections of SOD1G93A and WT mice. Red: denotes  NeuN, Green: denotes CRMP4. Scale bar: 10 μm. (D) Quantification of the percentage of CRMP4 positive SC neurons in 3 WT VS. 3 SOD1G93A mice. We monitored CRMP4 expression in total of 108 cells in WT condition and 123 cells in  SOD1G93A, an average of 36 or 41 cells in each repeat respectively. Student's t-test, n = 3, Data presented as mean ±SE, *p = 0.0161. "
NCBI gene IDs:
"SOD1: 6647"
Figure legend: "(C) Representative images of SOD1G93A/ChAT::tdTomato  or WTChAT::tdTomato neuromuscular junctions at P90. White: denotes  BTX, Red: denotes direct ChAT, Green: denotes CRMP4, Yellow: denotes Z projection of 3D Imaris  co-localization of CRMP4 and ChAT. Scale bar: 10 µm. (D) Quantification of CRMP4 positive NMJs in gastrocnemius muscles from 3 WT or 3 SOD1G93A P90  mice. Total of 44 NMJ's in WT condition and 60 NMJ's in  SOD1G93A condition. Student's t-test, n=3, Data presented as mean ±SE, *p = 0.0157. (E) Quantification of the percent of partially denervated  NMJ's  in the presence or absence of CRMP4 immunostaining  in 3 different SOD1G93A mice. We counted 24 NMJ's in SOD1G93A CRMP4 negatives and 67 NMJ's in SOD1G93A CRMP4  positives. Student's t-test, n=3, Data presented as mean ±SE, *p = 0.0352. "
NCBI gene IDs:
"tdTomato: 
ChAT: 12647
SOD1: 6647"
Figure legend: "(F) Representative  images  of P90  SOD1G93A and WT sciatic nerves. Red: denotes  NFH, Blue: denotes  GFAP  and green denotes CRMP4, Yellow: denotes the Z projection of 3D Imaris co-localization of CRMP4  and NFH. Scale bar: 5 μm. (G) Quantification of the co-localization  area of CRMP4 with NFH in the sciatic nerve in 3 SOD1G93A mice compared to 3 WT mice using Imaris analysis. 14 WT sciatic nerve sections and 11 SOD1G93A sections were monitored. Data presented as mean ±SE. Student's t-test, n=3, ****p&lt;0.0001. "
NCBI gene IDs:
"SOD1: 6647"
Figure legend: "(A) Representative images of healthy or C9orf72 iPSC-derived  MNs treated with Sema3A  or untreated in the distal compartment, 6 hours post treatment. Red: denotes Tubulin, Green: denotes CRMP4. Scale bar: 5 μm. (B) Quantification of CRMP4 intensity levels in healthy or C9orf72 iPSC-derived MNs with Sema3A treatment or untreated. 14 untreated healthy axons, 12 healthy axons with Sema3A treatment, 49 untreated C9orf72 axons  and 31 C9orf72 axons with Sema3A treatment were monitored from 3 different chambers. One-way ANOVA, Tukey's multiple comparisons test, n = 3, Data presented as mean ±SE, *p = 0.0338; ****p&lt;0.0001. "
NCBI gene IDs:
"C9orf72: 203228"
Figure legend: "(D) Representative images  of healthy or C9orf72 human-derived MN cell somata with Sema3A  treatment, Sema3A + dynein inhibitor treatment, or untreated. Gray: denotes CTX, Green: denotes CRMP4. Scale bar: 5μm. (E) Quantification of  CRMP4 intensity (normalized to GAPDH+mCherry/area) levels at the somata of healthy or C9orf72 human-derived MN  after Sema3A  treatment, Sema3A + dynein inhibitor treatment, or untreated. Analysis performed in 3 independent chambers per condition. 19 healthy untreated cell somata, 26 healthy cell somata with Sema3A treatment, 20 healthy cell somata with Sema3A  + dynein  inhibitor treatment and 14 C9orf72 cell somata from each condition were monitored. One-way ANOVA, Newman-Keuls multiple comparisons test, n=3, Data presented as mean ±SE, *p=0.0207; ***p=0.0004. "
NCBI gene IDs:
"C9orf72: 203228"
Figure legend: "(F) Representative images of healthy or C9orf72 human-derived MN proximal axons with Sema3A treatment, Sema3A + dynein inhibitor treatment or untreated control. Green: denotes CRMP4, Red: denotes GAPDH. Scale bar: 5μm. (G) Quantification of CRMP4 intensity levels (normalized to GAPDH+mCherry/area) at the proximal axons in healthy or C9orf72 human-derived MN after Sema3A treatment, Sema3A + dynein  inhibitor treatment, or untreated control. Analysis performed from 3 independent chambers in each condition. 21 healthy untreated proximal axons, 24 healthy proximal  axons  with Sema3A treatment, 16 healthy proximal  axons with Sema3A + dynein inhibitor treatment, 12 C9orf72  untreated proximal axons, 8 C9orf72 proximal axons with  Sema3A treatment and 13 C9orf72 proximal axons with Sema3A +  dynein  inhibitor treatment were monitored. One-way ANOVA, Newman-Keuls multiple comparisons test, n = 3, Data presented as mean ±SE, *p =0.0334, ****p&lt;0.0001. "
NCBI gene IDs:
"C9orf72: 203228"
Figure legend: "(F) Upper panel - Immunoprecipitation assay with anti‐Flag antibody followed by Western blot analysis of dynactin (p150) in COS7  cells overexpressing Flag-CRMP4 (size of ~65 KDa). Lower panel - Total input (size of ~150 KDa). (G) Quantification of the blot in F. The dynactin intensity  band was normalized to the Flag-CRMP4 intensity band in each repeat. Student's t-test, n=3, Data presented as mean ±SE, *p=0.0299. "
NCBI gene IDs:
"Flag: 
CRMP4: 22240"
Figure legend: "(H)  Immunoprecipitation of  DIC (size of ~75 KDa) followed by Western blot analysis of CRMP4  (size of ~64 KDa) in COS7 cells that were transfected with CRMP4 and AAV9-50aa or its control.  IgG antibody was used as a control. (I) Quantification of the blot  in H from 3 independent repeats. The CRMP4 intensity  band was normalized to the DIC intensity band in each repeat. Student's t-test, n=3, Data presented as mean ±SE, *p=0.0479. "
NCBI gene IDs:
"CRMP4: 22240"
Figure legend: "(E) Representative images from the proximity ligation assay (For explanation of  PLA  technique; please refer to method section) for CRMP4 and dynein in   SODG93A and WT primary  MNs axons that were exposed to either control or Sema3A 8h post treatment. Scale bar: 5µm. (F) Quantification of the  CRMP4-DIC puncta number per primary  motor neuron axon in each condition. We analyzed ~20 axons per condition from 3 independent chambers per group (One-way ANOVA, Tukey's multiple comparisons test, n=3, Data presented as mean ±SE, **p=0.01 *p=0.04) "
NCBI gene IDs:
"SOD: 6647"
Figure legend: "(G) Representative images of proximity ligation assay for  CRMP4 and dynein in healthy and C9orf72 human-derived proximal  axons post peptides treatment,  Sema3A treatment, Sema3A + peptides treatment or untreated controls. Scale bar: 5µm. (H) Quantification of the  CRMP4-DIC puncta number per axon in healthy or C9orf72 human-derived MN proximal  axons after  Sema3A treatment, Sema3A + peptides  treatment or untreated controls. Data collected from 3 independent chambers in each condition. Total of 37 healthy untreated proximal axons, 61 healthy proximal  axons with  peptides treatment, 59 healthy proximal  axons  with Sema3A treatment and 52 healthy proximal axons with Sema3A  + peptides treatment. 67 C9orf72  untreated proximal axons, 63  C9orf72 proximal  axons with peptides treatment, 41 C9orf72 proximal axons with Sema3A treatment and 50 C9orf72 proximal axons with Sema3A + peptides treatment monitored. Data presented as mean ±SE. One-way ANOVA, Tukey's multiple comparisons test, n=3, ****p&lt;0.0001. "
NCBI gene IDs:
"C9orf72: 203228"
Figure legend: "(A) Representative images of CTX signal in healthy and  C9orf72 human IPSC-derived  MNs before and after Sema3A application. Green: denotes CTX-positive cells. Yellow circles are numbered CTX positive cells. Purple circles are cells that are missing post Sema3A treatment. Scale bar: 40 μm. (B-C) Quantification of CTX  signal in healthy and C9orf72 IPSC-derived  MNs before and 3 days after applying Sema3A to distal compartment, compared with untreated control. 3 independent chambers in each condition were analyzed. Average of ~150 neurons per condition monitored. Student's t-test, n=3, Data presented as mean ±SE, *p&lt;0.05. "
NCBI gene IDs:
"C9orf72: 203228"
Figure legend: "(D) Representative images of CTX signal in WT or  SOD1G93A primary  MNs before and 2 days after Sema3A  application to the distal compartment in the presence of either Dynein inhibitor+Sema3A, Dynasore+Sema3A or untreated. Green: denotes  CTX-positive cells. Yellow circles are numbered CTX  positive cells. Purple circles are cells that are missing post Sema3A treatment. Scale bar: 30 μm. (E) Quantification of CTX signal in a SOD1G93A explant before and 2 days after  Sema3A  application to the distal compartment in the presence of either Dynein  inhibitor+Sema3A, Dynasore+Sema3A. 3 independent chambers in each condition were analyzed. ~200 neurons were monitored per each condition. One-way ANOVA, Tukey's multiple comparisons test, n = 3, Data presented as mean ±SE, *p&lt;0.05, **p&lt;0.01.  Dynein inhibitor and Dynasore treatments were used as a negative control. "
NCBI gene IDs:
"SOD1: 6647"
Figure legend: "(A) Representative images and insets of P90 WT and SOD1G93A SC cross sections at P90. Blue: denotes  DAPI, Red: denoted NeuN, and White: denotes activated caspase 3. Scale bar: 20 µm."
NCBI gene IDs:
"SOD1: 6647"
Figure legend: "(C) Representative images of P90 SOD1G93A mice SC  cross sections that were injected with AAV9-GFP/AAV9-50aa-GFP. Blue: denotes DAPI, Red: denoted NeuN, and White: denotes  activated caspase 3. Scale bar: 10 µm."
NCBI gene IDs:
"GFP: 
SOD1: 6647"
Figure legend: "(B) Expression of GFP, lncRNA-c1, GFP-lncRNA-c1, lncRNA-c2 and GFP-lncRNA-c2 (x-axis) in 8 day 4-OHT treated, miRNA depleted cells (KO) relative to ethanol treated (WT) mESC (y-axis) 24h hours post-transfection. Four independent biological replicates were treated, transfected and analyzed by RT-qPCR. Statistical significance represented on the figure based on comparison of KO/WT fold change in expression of GFP, with GFP-lncRNA-c1 and with GFP-lncRNA-c2, (paired two-tailed t-test p-value= 0.034 and 0.032 respectively) and based on comparison of KO/WT fold change in expression of GFP-lncRNAc1 with lncRNA-c1 and GFP-lncRNA-c2 with lncRNA-c2 (paired two-tailed t-test p-value= 0.012 and 0.018 respectively). Data is represented as mean±SD and Each point corresponds to the results of one independent biological replicate. Data information: For all RTqPCR analyses, transcript expression was first normalized by the amount of Act-β and PolII and next by the total amount of transfected vectors per cell estimated based on the levels of relative Neomycin expression. Each point corresponds to the results of one independent biological replicate. Statistics: NS- p-value&gt; 0.05, *-p-value&lt;0.05, **-p-value&lt;0.01 and ***-p-value&lt;0.001."
NCBI gene IDs:
"GFP: 
lncRNA-c1: 
lncRNA-c2: 
lncRNAc1: 
PolII: 
Act-β: "
Figure legend: "(C) Expression of GFP-lncRNA-c1 ΔMRE relative to GFP-lncRNA-c1 (y-axis) in ethanol treated (WT, circles) or 4-OHT treated, miRNA-depleted cells (KO, triangles, x-axis). Four independent biological replicates were treated, transfected and analyzed by RTqPCR. Data is represented as mean±SD and Each point corresponds to the results of one independent biological replicate. Paired two-tailed t-test p-value= 0.0071 Data information: For all RTqPCR analyses, transcript expression was first normalized by the amount of Act-β and PolII and next by the total amount of transfected vectors per cell estimated based on the levels of relative Neomycin expression. Each point corresponds to the results of one independent biological replicate. Statistics: NS- p-value&gt; 0.05, *-p-value&lt;0.05, **-p-value&lt;0.01 and ***-p-value&lt;0.001."
NCBI gene IDs:
"Act-β: 
lncRNA-c1: 
PolII: 
GFP: "
Figure legend: "(F) Immunoblot analysis of GFP (GFP) in protein extracts from mESCs transfected with mock, BoxB(-30)-GFP and GFP expressing vectors. ACTIN-β (ACT-β) was used as an internal control. One representative blot is depicted (G) Fold-change (FC) in normalized expression of GFP-lncRNA-c1, GFP-lncRNA-c2 (noBoxB, circles) and BoxB(-30)-GFP-lncRNA-c1, BoxB (-30)-GFP-lncRNA-c2 (BoxB(-30), triangles) (x-axis) 4-OHT treated, miRNA depleted mESCs (KO) relative to ethanol treated mESCs (WT) (y-axis). Four independent biological replicates were analyzed. Data is represented as mean±SD and Each point corresponds to the results of one independent biological replicate. Paired two-tailed t-test p-value= 0.0494 for GFP-lncRNA-c1 and p-value=0.0355 for GPF-lncRNA-c2Data information: Uncropped blots used for assembly of panels F are provided in Figure 4 Source Data."
NCBI gene IDs:
"lncRNA-c2: 
BoxB: 
GFP: 
GPF: 
lncRNA-c1: "
Figure legend: "(H) Immunoblot analysis of GFP (GFP) in protein extracts from mESCs transfected with mock, BoxB(+339)-GFP and GFP expressing vectors. ACTIN-β (ACT-β) was used as an internal control. One representative blot is depicted. (I) Fold-change (FC) in normalized expression of GFP-lncRNA-c1, GFP-lncRNA-c2 (noBoxB, circles) and BoxB(+339)-GFP-lncRNA-c1, BoxB (+339)-GFP-lncRNA-c2 (BoxB(+339), triangles) (x-axis) in 8 day 4-OHT treated, miRNA depleted mESCs (KO) relative to ethanol treated mESCs (WT) (y-axis). Four independent biological replicates analyzed.Data information: Uncropped blots used for assembly of panels H are provided in Figure 4 Source Data."
NCBI gene IDs:
"lncRNA-c2: 
BoxB: 
GFP: 
lncRNA-c1: "
Figure legend: "A-B Representative image of a proliferative cell in the DG labeled with an anti-PH3 antibody (green) (A) and quantification of the number of PH3+ cells in the DG of WT and Tau-/- mice (B) (mean ± SEM; n= 10 mice WT, n= 8 mice Tau-/-; Student´s t-test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "C-D Representative image of an apoptotic cell in the DG labeled with an anti-fractin antibody (red) (C) and quantification of the number of fractin+ cells in the DG of WT and Tau-/- mice (D) (mean ± SEM; n= 10 mice WT, n= 8 mice Tau-/-; Student´s t-test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "E-F Schematic diagram of the cell survival experimental design using thymidine analogs (F) and quantification of the percentage of surviving cells in Tau-/- mice as compared to WT mice at each cell age (1-, 2-, 4-, 6- and 8-week-old cells) (F) (mean ± SEM (normalized data); Student´s t-test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "H Quantification of the percentage of 1-, 4- and 8-week-old newborn neurons that express the DCX neuroblast marker and the NeuN mature neuron marker in WT and Tau-/- mice (χ2 test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "I-J Representative images of progenitor cells in the DG labeled with anti-Sox2 (red) and anti-BLBP (green) antibodies (I), and neuroblasts and immature neurons labeled with anti-DCX (red) and anti-calretinin (green) antibodies, respectively (J).K-N Quantification of the number of Sox2+ (K), BLBP+ (L), DCX+ (M) and calretinin+ (N) cells in the DG of WT and Tau-/- mice (mean ± SEM; n= 10 mice WT, n= 8 mice Tau-/-; Student´s t-test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "A Representative images of 4-week-old newborn granule neurons of WT and Tau-/- mice infected by a PSD95-GFP-expressing retrovirus. A schematic representation of Sholl´s analysis is shown in the WT image.B-C Quantification of total dendritic length (B) and Sholl´s analysis (C) of 4-week-old newborn granule neurons in WT and Tau-/- mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "D Representative images of 8-week-old newborn granule neurons of WT and Tau-/- mice infected by a PSD95-GFP-expressing retrovirus.E-F Quantification of total dendritic length (E) and Sholl´s analysis (F) of 8-week-old newborn granule neurons in WT and Tau-/- mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "G Representative images of 4-week-old newborn granule neurons of WT and Tau-/- mice infected by a PSD95-GFP-expressing retrovirus and their corresponding high-power magnifications showing PSDs (green).H-IQuantification of the number of PSDs/µm (H) and PSD area (I) in each dendritic branching order of 4-week-old newborn granule neurons in WT and Tau-/- mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "J Representative images of 8-week-old newborn granule neurons of WT and Tau-/- mice infected by a PSD95-GFP-expressing retrovirus and their corresponding high-power magnifications showing PSDs (green).K-L Quantification of the number of PSDs/µm (K) and PSD area (L) in each dendritic branching order of 8-week-old newborn granule neurons in WT and Tau-/- mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "A Representative traces of mEPSCs recorded at a holding potential of −65 mV from WT (black traces) and Tau-/- (red traces) mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "B Exemplary traces of mEPSCs from one cell from WT (black trace) and Tau-/- (red trace) mice."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "C Resting membrane potential of WT and Tau-/- mice (mean ± SEM; n= 13 cells WT, n= 10 cells Tau-/-; *0.05 &gt; p ≥ 0.01, Student´s t-test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "D-E mEPSC amplitude (D) and mEPSC frequency (E) of WT and Tau-/- animals (mean ± SEM; n= 13 cells WT, n= 10 cells Tau-/-; Student´s t test or Mann-Whitney´s U test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "F Cumulative probability plot of the total mEPSC amplitudes, small mEPSCs (i) and big mEPSCs (ii) from both WT and Tau-/- mice (black and red traces, respectively) (***p &lt; 0.001; Kolmogorov-Smirnov Z test)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "C Representative images of 1-week-old newborn granule neurons labeled with CldU (green) belonging to WT CNP, WT P, Tau-/- CNP, and Tau-/- P mice. Cell nuclei were labeled with DAPI (blue). Scale bar 50 µm.D-E Quantification of the number of 1-week-old CldU+ cells (D) and apoptotic fractin+ cells (E) in each experimental group."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "B Representative images of 8-week-old newborn granule neurons of WT CNP, WT P, Tau-/- CNP, and Tau-/- P mice infected with a PSD95-GFP-expressing retrovirus. In the WT CNP image a schematic representation of Sholl´s analysis is shown.C-D Quantification of total dendritic length (C) and Sholl´s analysis (D) of 8-week-old newborn granule neurons (n= 3 mice per experimental condition)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "E-F Representative images of an 8-week-old newborn granule neuron, in which proximal dendrites are highlighted (E), and of PSDs (green) in proximal dendrites belonging to WT CNP, WT P, Tau-/- CNP, and Tau-/- P mice (F).G-H Quantification of the number of PSDs/µm (G) and PSD area (H) in each dendritic branching order of 8-week-old newborn granule neurons (n= 3 mice per experimental condition)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "I-J Representative Western Blot (I) and quantification (J) of the levels of GluR1 subunit of AMPA receptors in hippocampi of WT and Tau-/- mice subjected or not to the Porsolt test (n= 6 mice per experimental condition)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "L-M Representative images (L) and quantification of the area (M) of MFTs of 8-week-old newborn neurons in the CA3 region of WT CNP, WT P, Tau-/- CNP, and Tau-/- P mice (n= 3 mice per experimental condition). Arrows indicate MFTs."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "C-D Representative images of DCX+ cells in the DG of CH and EE animals (C), and quantification of the number of DCX+ cells of WT and Tau-/- animals in CH or EE conditions (D)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "E Representative images of 8-week-old newborn granule neurons labeled with IdU (red) belonging to WT CH, WT EE, Tau-/- CH and Tau-/- EE mice.F-G Quantification of the number of 8-week-old IdU+ cells (F) and apoptotic fractin+ cells (G)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "A Representative images of the whole hippocampus showing GABAergic innervation labeled with GAD-65 antibody (red), and high-power magnifications of the DG molecular layer showing GAD-65+ terminals in WT CH, Tau-/- CH, WT EE and Tau-/- EE mice. Cell nuclei were labeled with DAPI (blue). White scale bar 300 µm. Red scale bar 50 µm. Green scale bar 5 µm.B-C Quantification of the density (B) and area (C) of GAD-65+ terminals in the molecular layer of mice in each experimental condition (n= 10 mice WT CH, n= 8 miceTau-/- CH, n= 6 mice WT EE, n= 10 miceTau-/- EE)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "B Representative images of 8-week-old newborn granule neurons of WT CH, WT EE, Tau-/- CH, and Tau-/- EE mice infected with a PSD95-GFP-expressing retrovirus. In the WT CH image a schematic representation of Sholl´s analysis is shown.C-D Quantification of total dendritic length (C) and Sholl´s analysis (D) of 8-week-old newborn granule neurons (n= 3 mice per experimental condition)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "E-F Representative images of an 8-week-old newborn granule neuron in which proximal and distal dendrites are highlighted (E) and of PSDs (green) in distal and proximal dendrites of WT and Tau-/- mice housed under either CH or EE conditions (F).G-H Quantification of the number of PSDs/µm (G) and PSD area (H) in each dendritic branching order of 8-week-old newborn granule neurons (n= 3 mice per experimental condition)."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "J-K Representative images (J) and quantification of the area (K) of MFTs of 8-week-old newborn neurons in the CA3 region of WT CH, WT EE, Tau-/- CH, and Tau-/- EE mice (n= 3 mice per experimental condition). Arrows indicate MFTs."
NCBI gene IDs:
"Tau: 17762"
Figure legend: "GFP-ATG8a expressing seedlings in Murashige &amp; Skoog (MS) growth medium or 30 min after treatment with MS containing ACC, ABA, ATP, BL, 6-BA, Flg22, NAA or PEP1. (A) Representative maximum intensity projection images of 10 Z-stacks per image. Scale bar: 10 µm."
NCBI gene IDs:
"GFP: 
ATG8a: 828287"
Figure legend: "GFP-ATG8a expressing seedlings in Murashige &amp; Skoog (MS) growth medium or 30 min after treatment with MS containing ACC, ABA, ATP, BL, 6-BA, Flg22, NAA or PEP1. (B) Quantification of GFP foci per 0.0025 mm2. Values are presented as mean ± standard deviation of the mean and were calculated from at least three independent experiments with 3 individuals per replicate. Bars marked with an asterisk (*) are statistically significant (P&lt;0.05) according to the T -test."
NCBI gene IDs:
"GFP: 
ATG8a: 828287"