EMBO/SourceData huggingface dataset:
Figure legend: "WT primary hepatocytes were infected with an adenovirus harboring FERM-Myc (Ad-FERM-Myc) or LacZ (Ad-LacZ) for 48 hours. (F) Ad-FERM-Myc- or Ad-LacZ-expressing WT hepatocytes were serum-starved for 16 hours and stimulated with glucagon (10 nM) for 10 minutes. Levels of p-PKA substrates, p-CREB, CREB, FERM-Myc and GAPDH were determined by immunoblotting."
NCBI gene IDs:
"LacZ: 
Myc: "
Figure legend: "Twelve-week-old male wild type mice were infected with an adenovirus harboring GFP or FERM for 3 days, fasted for 4 hours and intraperitoneally injected with glucagon. (G) Blood glucose levels (mg/dL) were measured 0, 15, 30, 60 and 120 minutes after glucagon stimulation (0.2 mg/kg body weight, n = 10 mice/ group)."
NCBI gene IDs:
"GFP: "
Figure legend: "Twelve-week-old male wild type mice were infected with an adenovirus harboring GFP or FERM for 3 days, fasted for 4 hours and intraperitoneally injected with glucagon. (H) Levels of p-CREB, CREB, p-PKA substrates, and FERM in the liver 10 minutes after glucagon stimulation (2 mg/kg body weight) were determined by immunoblotting (n = 3 mice/ group)."
NCBI gene IDs:
"GFP: "
Figure legend: "Twelve-week-old male wild type mice were infected with an adenovirus harboring GFP or FERM for 3 days, fasted for 4 hours and intraperitoneally injected with glucagon. (I) Glucose disposal rates in GFP- or FERM-overexpressing mice were measured using glucose, pyruvate and insulin tolerance tests (n = 10 mice/group)."
NCBI gene IDs:
"GFP: "
Figure legend: "(A) Wild type primary hepatocytes were infected with an adenovirus harboring sh-control (sh-Con), sh-radixin (sh-Rdx), GFP (Ad-GFP), and/or sh-resistant wildtype radixin (Ad-RdxR) or AKAP-dead LPTD mutant of radixin (Ad-LPTDR) for 24 hours."
NCBI gene IDs:
"AKAP: 
GFP: 
radixin: 19684
Rdx: 19684"
Figure legend: "(B) Increasing amount of Ad-RdxR or Ad-LPTDR were infected to WT primary hepatocytes for 24 hours. Hepatocytes were further serum-starved for 18 hours and stimulated with glucagon (10 nM) for 10 minutes. Levels of p-PKA substrates, p-CREB, CREB, radixin, Myc-radixin and GAPDH were determined by immunoblotting."
NCBI gene IDs:
"Rdx: 19684"
Figure legend: "Four-week-old male WT (Prom1+/+) and KO (Prom1-/-) mice were fed a high-fat diet for 8 weeks (Diet-Induced Obesity; DIO), and fasted for 4 hours before experiments. (C) DIO WT (Prom1+/+) and KO (Prom1-/-) mice were intraperitoneally injected with glucagon. Blood glucose levels (mg/dL) were measured 0, 15, 30, 60 and 120 minutes after glucagon (100 µg/kg body weight) stimulation (n = 10 mice/ group)."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Four-week-old male WT (Prom1+/+) and KO (Prom1-/-) mice were fed a high-fat diet for 8 weeks (Diet-Induced Obesity; DIO), and fasted for 4 hours before experiments. (D) Levels of p-PKA substrates, p-CREB, CREB, Prom1 and GAPDH were determined by immunoblotting 10 minutes after glucagon (2 mg/kg body weight) stimulation (n = 3 mice/group)."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Four-week-old male WT (Prom1+/+) and KO (Prom1-/-) mice were fed a high-fat diet for 8 weeks (Diet-Induced Obesity; DIO), and fasted for 4 hours before experiments. (E) Glucose disposal rates in DIO WT (Prom1+/+) and KO (Prom1-/-) mice were measured using glucose and insulin tolerance tests (n = 10 mice/group)."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Four-week-old male WT (Prom1+/+) and KO (Prom1-/-) mice were fed a high-fat diet for 8 weeks (Diet-Induced Obesity; DIO), and fasted for 4 hours before experiments. (F) DIO KO (Prom1-/-) mice were infected with an adenovirus harboring LacZ or Prom1 for 24 hours, fasted for 4 hours and intraperitoneally injected with glucagon (100 µg/kg body weight). Blood glucose levels (mg/dL) were measured 0, 15, 30, 60 and 120 minutes after glucagon stimulation (GST). Glucose disposal rates were measured using the pyruvate tolerance test (PTT) (n = 10 mice/group)."
NCBI gene IDs:
"LacZ: 
Prom1: 19126"
Figure legend: "(B) Western blot  of RNAi efficiency and specificity for oligos used in (C-G). HeLa Kyoto cells were incubated for 72 h with control oligos or RNAi-oligos depleting CUL4A, CUL4B  or DDB1. Specific downregulation of the indicated proteins was analyzed by immunoblotting. GAPDH  controls for equal loading. (C) Quantification of  western blot bands and the ratio between neddylated  vs unneddylated CUL4 with the paralog downregulated is calculated. Graphs displays mean with SEM of N=3 biological replicates. **P = 0.001."
NCBI gene IDs:
"CUL4A: 8451
CUL4B: 8450
DDB1: 1642"
Figure legend: "(A)  Western blot  analysis to monitor CUL4A  or CUL4B  levels in extracts prepared from HeLaFLP  control cells or HeLaFLP  cells deleted for CUL4B by CRISPR/Cas9  (∆CUL4BFLP) but stably expressing no CUL4B  (-), CUL4B  or the indicated NP1 or P50L mutants from the doxycycline-inducible promoter. GAPDH  was included to control equal loading. One of three biological replicates is shown."
NCBI gene IDs:
"CRISPR: 
Cas9: 69900935
CUL4B: 8450"
Figure legend: "(C) To control RNAi efficiency and specificity, HeLa Kyoto cells were incubated for 72 h with control oligos (control) or RNAi-oligos depleting CUL4B, LIS1  or WDR1. Specific downregulation of the indicated proteins was analyzed by immunoblotting. GAPDH  controls for equal loading. One of three biological replicates is shown."
NCBI gene IDs:
"CUL4B: 8450
LIS1: 5048
WDR1: 9948"
Figure legend: "(E) HA-immunoprecipitation  (IP) of extracts (input) prepared from unedited (-) or HA-CUL4B  endogenously expressing HeLa Kyoto  cell lines arrested in S-phase (thymidine) or mitosis  (nocodazole) were probed with antibodies against LIS1, WDR1  and DDB1. The antibody recognizing phospho-H3(S19) controls mitotic  arrest and specificity of the immunoprecipitation."
NCBI gene IDs:
"HA: 
CUL4B: 8450"
Figure legend: "(F, G)  HeLa Kyoto  endogenously expressing HA-CUL4B  or the indicated NP1 or P50L mutants were arrested in mitosis with nocodazole. Extracts (input) and HA-immunoprecipitants  were analyzed for the presence of HA-CUL4B  for control and bound DDB1  and LIS1  (F) or DDB1  and WDR1  (G), respectively. One of 3 biological replicates is shown."
NCBI gene IDs:
"HA: 
CUL4B: 8450"
Figure legend: "(C) Extracts (input) prepared from S-phase (S; thymidine) or mitosis  (M; nocodazole)-arrested  HeLa Kyoto  cells (-) and endogenously expressing HA-tagged WT CUL4B, or the NP1 or P50L mutants were immunoprecipitated  with HA.II-antibodies (IP). Bound ACTR3  or DDB1  was detected using specific antibodies. One of three biological replicates is shown. Note that in contrast to DDB1, ACTR3 binds CUL4B  in a phosphorylation-dependent manner."
NCBI gene IDs:
"HA: 
CUL4B: 8450"
Figure legend: "(D) HA-immunoprecipitants   (IP) of extracts (input) prepared from unedited (-) controls or endogenously expressing HA-CUL4B HeLa Kyoto  cells arrested in mitosis  with nocodazole  were probed with antibodies against DDB1, DCTN1  (dynactin) and actin. One of two biological replicates is depicted."
NCBI gene IDs:
"HA: 
CUL4B: 8450"
Figure legend: "(F) Live cell imaging of HeLa  cells expressing H2B-mcherry   and lifeAct-GFP, treated for 72 h with control oligos (siControl) or RNAi-oligos depleting CUL4B, LIS1  or WDR1. Representative cells are shown in time  intervals of 6 min during metaphase with identical contrast settings. The unanchored spindle  in CUL4B- and LIS1  deleted cells is marked with a white arrow. Scale bar: 10 μm."
NCBI gene IDs:
"CUL4B: 8450
LIS1: 5048
WDR1: 9948"
Figure legend: "hESCs  were differentiated to generate human forebrain  specific regionalised organoids. Sections were stained  as indicated with antibodies against SOX2  or CTIP2  to visualize neural stem cell  and neuronal cell populations, respectively. (A) Example images of day 35 organoids. The stained  sections are also shown as merged images at different maginifications, with scale bars of 200 μm and 50 μm, respectively. (B) The number of cortical  units per mm2 total area was quantified in unedited and ΔCUL4B brain  organoids at day 35. Ventricles  were counted and the number divided by the total organoid area on 20 sections from one biological replicate."
NCBI gene IDs:
"CUL4B: 8450"
Figure legend: "A Time course images of the hippocampal CA1 region of Siglechdtr/dtr mice after DT administration. Sections were stained with an anti-Iba1 antibody (green) and DAPI (cyan). Arrow: live microglia with normal nucleus. Double arrow: apoptotic microglia with pyknotic nucleus. Arrowhead: microglial debris with no nucleus. Scale bar, 50 µm. B Number of apoptotic microglia with pyknotic or fragmented nucleus (n = 5 animals per group, Kruskal-Wallis test with post hoc Dunn's test). C Number of live microglia with normal nucleus (n = 5 animals per group, one-way ANOVA with post hoc Tukey's test). D Number of pieces of microglial debris (Iba1+ spheres with a diameter &gt; 2 µm and no nucleus) (n = 5 animals per group, Kruskal-Wallis test with post hoc Dunn's test). "
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "E A representative image of a survived microglial cell in the hippocampal CA1 region of Siglechdtr/dtr mice 2 days after DT administration. Sections were stained with an anti-Iba1 antibody (green) and DAPI (cyan). A 3D image (rightmost panel) was reconstructed using Imaris software. Arrow: live microglia. Arrowhead: microglial debris. Scale bar, 10 µm."
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "A Histological analysis of RFP+ cells in Siglechdtr/dtr:Ccr2RFP/+ mice. Lumber spinal cord after EAE induction, and hippocampal CA1 2 or 4 days after PBS or DT injection were analyzed. Iba1 immunoreactivity (green) and RFP fluorescence (red) are shown. Images were acquired using the same laser power and sensitivity, and image processing was the same for all RFP signals (red). Scale bar, 50 µm."
NCBI gene IDs:
"RFP: 
Ccr2: 12772
dtr: 15200
Siglech: 233274"
Figure legend: "B Flow cytometric analysis of RFP+Ly6Chigh inflammatory monocytes in peripheral blood of Siglechdtr/dtr:Ccr2RFP/+ mice 2 days after PBS or DT administration. Representative data and a quantification graph (n = 5 animals per group, two-tailed unpaired Student's t-test) are shown."
NCBI gene IDs:
"RFP: 
Ccr2: 12772
dtr: 15200
Siglech: 233274"
Figure legend: "C Immunohistochemical localization of CD206+ perivascular macrophages (arrows) in hippocampal CA1 of Siglechdtr/dtr mice. Sections prepared 2 days after administration of PBS or DT were stained with anti-Iba1 (green), anti-CD206 (red) and anti-CD31 (blue) antibodies. Scale bar, 50 µm. D Number of perivascular macrophages in hippocampal CA1 of Siglechdtr/dtr mice 2 days after PBS or DT administration (n = 5 animals per group, two-tailed unpaired Student's t-test). "
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "A qPCR analysis of marker molecules for CNS cell types. The hippocampus of WT and Siglechdtr/dtr mice 2 days after PBS or DT administration was analyzed (n = 3 animals per group, one-way ANOVA with post hoc Tukey's test). Results are normalized to Gapdh, and are shown as ratios to the value of WT mice injected with PBS."
NCBI gene IDs:
"Gapdh: 14433
dtr: 15200
Siglech: 233274"
Figure legend: "B Immunohistochemical detection of astrocytes in Siglechdtr/dtr mice. Hippocampal CA1 sections were prepared 2 days after PBS or DT administration, and stained with an anti-GFAP antibody. Images were acquired using the same laser power and sensitivity, and image processing was the same. Scale bar, 30 µm. C Astrocyte number in hippocampal CA1 of Siglechdtr/dtr mice 2 days after PBS or DT administration (n = 5 animals, two-tailed unpaired Student's t-test). "
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "A Representative images of microglial debris surrounded by astrocyte processes in hippocampal CA1. Sections were prepared from Siglechdtr/dtr mice 2 days after PBS or DT administration, and stained with anti-GFAP (green) and anti-CD11b (red) antibodies. 3D images (lower row) were reconstructed from confocal images (upper row) using Imaris software. Scale bar, 10 µm."
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "B CLEM analysis of a phagocytic astrocyte in hippocampal CA1 of Siglechdtr/dtr mice 2 days after DT administration. A phagocytic astrocyte, which engulfed degenerated microglial components, was identified by immunohistochemistry using anti-S100β (green) and anti-CD11b (red) antibodies, and DAPI (cyan) (upper and middle panels). Then, a phagocytic cup of the same cell was analyzed by electron microscopy (lower panel). Degenerated microglial cytoplasm/plasma membrane (arrow) and nucleus (double arrow) in a phagocytic cup of the astrocyte, and a piece of microglial debris internalized in astrocytic cytoplasm (arrowhead) are indicated. Scale bar, 50 µm (upper panel), 10 µm (upper panel [inset] and middle panel), and 2 µm (lower panel)."
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "C A representative image of a phagocytic astrocyte in hippocampal CA1 of Siglechdtr/dtr mice 2 days after DT administration. Sections were stained with anti-S100β (green) and anti-CD11b (red) antibodies, and confocal images were reconstructed using Imaris software. The lower two panels are higher magnification images of contacted microglial debris. Arrow: microglial debris contacted by astrocyte processes (coverage: approximately 30 or 50%). Arrowhead: microglial debris internalized in astrocyte cytoplasm. Scale bar, 10 µm (upper panel) and 2 µm (lower panels). D, E Quantification of the pieces of debris (CD11b+ spheres with &gt; 0.5 µm diameter) contacted with (coverage &gt; 30%) (D) and internalized by (E) a single astrocyte within 30 µm-thick sections (n = 75 cells from five animals per group). The number of debris pieces was counted in 3D images reconstructed using Imaris software. All confocal images were acquired using the same laser power and sensitivity, and image processing was the same."
NCBI gene IDs:
"dtr: 15200
Siglech: 233274"
Figure legend: "Cultured astrocytes engulf microglial debris. CAG-EGFP mouse-derived primary astrocytes were co-cultured with or without fluorescence-labelled apoptotic microglia. (A) Fluorescence images of GFP (green), microglial debris (red) and GFAP immunoreactivity (blue). Images were acquired using the same laser power and sensitivity, and image processing was the same in the two images (red). Scale bar, 50 µm."
NCBI gene IDs:
"EGFP: "
Figure legend: "D Expression of TAM family members and their knockdown by siRNA in cultured astrocytes. Tyro3, Axl and Metk in cultured astrocytes or hippocampal tissue were examined by western blotting. Arrows indicate the corresponding protein band(s). The secondary antibody against goat IgG generates a ~115 kDa non-specific band. The ~115 kDa band in the Axl image is the Axl-specific band overlapping the non-specific band. The ~115 kDa band in the Mertk image is the non-specific band. Axl and Mertk expression was knocked down by two siRNAs. GAPDH expression is shown as an internal control. E Quantification of siRNA knockdown in cultured astrocytes (n = 3 independent experiments, one-way ANOVA with repeated measures). Knockdown of Tyro3 mRNA was examined by qPCR because of the very low expression level even in control astrocytes. Knockdown efficiency of Axl and Mertk was quantified from western blots. Results are normalized to Gapdh or GAPDH, and are shown as ratios to the value of non-transfected astrocytes."
NCBI gene IDs:
"Axl: 26362
GAPDH: 14433
Mertk: 17289
Tyro3: 22174"
Figure legend: "B Representative images of microglial distribution in the cerebral cortex of WT and mutant mice at 3 w. Sections were stained with anti-Iba1 or anti-CD11b antibodies (red). Scale bar, 100 µm. C Quantification of microglial number (n = 5 animals per group, one-way ANOVA). Note that Irf8-/- mice were analyzed by CD11b immunostaining, while other strains were analyzed by Iba1 staining. "
NCBI gene IDs:
"Irf8: 15900"
Figure legend: " D Representative images of a spontaneous apoptotic cell and surrounding glial cells in the cerebral cortex of WT and Irf8-/- mice at 3 w. Sections were stained with anti-GFAP (green), anti-CD11b (red) and anti-cleaved caspase-3 (blue) antibodies. 3D images (lower row) were reconstructed from confocal images (upper row) using Imaris software. Arrows indicate cleaved caspase-3+ apoptotic cells. Insets are higher magnification images around apoptotic cells. Scale bar, 20 µm and 5 µm (insets). E Percentage of spontaneous apoptotic cells surrounded by microglia in WT and mutant mice. 3D images reconstructed from confocal images were analyzed (n = 36-50 cells from five animals per group, Kruskal-Wallis test with post hoc Dunn's test). F Percentage of spontaneous apoptotic cells surrounded by astrocyte processes in WT and mutant mice (n = 36-50 cells from five animals per group, one-way ANOVA with post hoc Tukey's test). "
NCBI gene IDs:
"Irf8: 15900"
Figure legend: "(C) Western blot analysis of cells transfected with 10 nM siRNA against ERK1, ERK2 or a 5 nM combination of each (ERK1+2)."
NCBI gene IDs:
"ERK2: 26413
ERK1: 26417"
Figure legend: "(a) Lysates extracted from the SVZ of three different Ctrl and FIP200GFAP cKO mice treated with chloroquine, and then analyzed by western blot using antibodies to LC3 (top), p62 (middle) or vinculin (bottom)."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(b) Immunofluorescence for p62 and DAPI in the SVZ and DG of Ctrl and FIP200GFAP cKO mice at P28 (n = 3 p62 for each). Dotted lines indicate boundaries of the SVZ and granular zone (GZ). The arrows and arrowheads mark larger and smaller p62+ aggregates, respectively, in the striatum (ST) and SVZ."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(c) TEM of mitochondria in SVZ tissue from Ctrl and FIP200GFAP cKO mice at P28 and P56 (n = 2 mice for each, &gt;30 cells for each mouse). Arrows mark mitochondria."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(d) TEM of mitochondria in cultured neurospheres from Ctrl and FIP200GFAP cKO mice (n = 2 mice for each, &gt;30 cells for each mouse). Arrows mark mitochondria."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(e,f) DHE and DAPI fluorescence in the dentate gyrus (e) and SVZ (f) of Ctrl and FIP200GFAP cKO mice at P28 (n = 5 mice for each). Dotted lines indicate the boundaries of the GZ (e) and SVZ (f). Arrows mark cells in the SGZ (e) and SVZ (f), and arrowheads mark cells in surround regions (the GZ in e and the ST in f). LV, lateral ventricle; ML, molecular layer. Full-length blots are presented in Supplementary Figure 11."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(a) Hematoxylin and eosin staining of the dentate gyrus (DG) and SVZ from Ctrl and FIP200GFAP cKO mice at P28. Lines indicate the boundaries of the DG and SVZ. Arrows mark cells in the SVZ."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(b-e) Immunofluorescence for GFAP, nestin or SOX2 and DAPI in the DG (b,c) from Ctrl and FIP200GFAP cKO mice at P28. The boxed areas in c-e are shown in more detail in insets (c). Dotted lines indicate boundaries of the granular zone (GZ) and SGZ (b). Arrows mark GFAP+nestin+ and GFAP+SOX2+ NSCs with radial glial morphology (b,c), and arrowheads mark GFAP+nestin− and GFAP+SOX2− astrocytes."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(b-e) Immunofluorescence for GFAP, nestin and DAPI in the SVZ (d,e) from Ctrl and FIP200GFAP cKO mice at P28. The boxed areas in c-e are shown in more detail in panels below (d,e). Dotted lines indicate boundaries of the SVZ (d,e). Arrows mark GFAP+nestin+ and GFAP+SOX2+ NSCs with radial glial morphology (b,c), and arrowheads mark GFAP+nestin− and GFAP+SOX2− astrocytes."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(b-e) Immunofluorescence for GFAP, nestin or SOX2 and DAPI in the SVZ (d,e) from Ctrl and FIP200GFAP cKO mice at P28. The boxed areas in c-e are shown in more detail in panels below (d,e). Dotted lines indicate boundaries of the SVZ (d,e). Arrows mark GFAP+nestin+ and GFAP+SOX2+ NSCs with radial glial morphology (b,c), and arrowheads mark GFAP+nestin− and GFAP+SOX2− astrocytes."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(f) Immunofluorescence for PSA-NCAM in the RMS (boxed areas), SVZ and DG of Ctrl and FIP200GFAP cKO mice at P28. Lines indicate the boundaries of the DG and SVZ. Arrows mark PSA-NCAM+ cells in the RMS, SVZ and DG."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(g) Immunofluorescence for NeuN in the olfactory bulb (OB) and DG of Ctrl and FIP200GFAP cKO mice at P31. Lines indicate the layers of the OB and boundaries of the DG. Boxed areas in top panels are shown in more detail for staining of NeuN in insets. CC, corpus callosum; E, ependymal cells; LV, lateral ventricle; ML, molecular layer; ST, striatum; IGL, internal granular layer; MCL, mitral cell layer; EPL, external plexiform layer; GL, glomerular layer."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(a-e) Primary (1st) and secondary (2nd) neurospheres from Ctrl and FIP200GFAP cKO mice at P0 and P28. Representative P0 phase contrast images are shown in a. Mean ± s.e.m. of number (b,d) and size (c,e) of primary and secondary neurospheres from three independent experiments are shown (n = 3 mice for each)."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(f) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DAPI staining of the SVZ and dentate gyrus (DG) from Ctrl and FIP200GFAP cKO mice at P28. Lines indicate the boundaries of the SVZ. Numbered boxed areas are shown in more detail as insets for staining of TUNEL and DAPI in the SVZ and DG."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(g) Short-term BrdU incorporation in the SVZ and DG of Ctrl and FIP200GFAPcKO mice at P28. Dotted lines indicate the boundaries of the SVZ and granular zone (GZ). Boxed areas are shown in more detail in the insets. Lines indicate the boundaries of the SVZ and GZ. Arrows mark BrdU+ cells. CC, corpus callosum; LV, lateral ventricle; ST, striatum. *P 0.05; **P 0.01; ***P 0.001."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(h) Long-term retention of BrdU in the SVZ and dentate gyrus of Ctrl and FIP200GFAP cKO mice at P31. Lines indicate the boundaries of the SVZ and GZ. Arrows mark BrdU+ cells. CC, corpus callosum; LV, lateral ventricle; ST, striatum. *P 0.05; **P 0.01; ***P 0.001."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421"
Figure legend: "(a) Lysates extracted from neurospheres of Ctrl, FIP200GFAP cKO, Trp53GFAP cKO and 2cKO mice and analyzed by western blot using antibodies to p53 and actin."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421
Trp53: 22059"
Figure legend: "(b) Mean ± s.e.m. of relative mRNA level (normalized to Ctrl mice as 1) of Cdkn1a, Bbc3, Fas, Pax6 and Stat3 in neurospheres of Ctrl, FIP200GFAP cKO, 2cKO and Trp53GFAP cKO mice are shown (n = 3 mice for each)."
NCBI gene IDs:
"Bbc3: 170770
Cdkn1a: 12575
Fas: 14102
GFAP: 14580
Pax6: 18508
FIP200: 12421
Stat3: 20848
Trp53: 22059"
Figure legend: "(c,d) Mean ± s.e.m. of the number (c) and size (d) of primary and secondary neurospheres from Ctrl, FIP200GFAP cKO, 2cKO and Trp53GFAP cKO mice at P28 (n = 3 mice for each)."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421
Trp53: 22059"
Figure legend: "(e-h) Analysis of immunofluorescence or staining on frozen-sectioned neurospheres for nestin, Ki67, TUNEL, PAX6, phosphorylated STAT3 (pSTAT3) and DAPI, as indicated. Mean ± s.e.m. of the percentage of Ki67+ (e), BrdU+ (f) and TUNEL+ (g) cells in neurospheres from Ctrl, FIP200GFAP cKO, 2cKO and Trp53GFAP cKO mice are shown (n = 3 mice for each, counting of &gt;500 cells for each mouse). Representative images are shown in h. *P 0.01. Full-length blots are presented in Supplementary Figure 11."
NCBI gene IDs:
"GFAP: 14580
FIP200: 12421
Trp53: 22059"