EMBO/SourceData huggingface dataset:
Figure legend: "B. Double immunofluorescence staining of retinas from RBEWT/WT and RBEKO/KO mice for PSD95 (green, to label postsynaptic specializations) and mGluR6 (red, to label specifically postsynaptic sites formed by bipolar neurons in photoreceptorsynapses). Abbreviations are as in A. Scale bars: 20 µm (left and right overview), 5 µm (left panel magnification), 2 µm (right panel magnification).C. Same as B, except that retinas were stained for PKCα (green, to label bipolar neurons) and PSD95 (red, to label amacrine cells)."
NCBI gene IDs:
"RBE: "
Figure legend: "A and B. Double immunofluorescence staining of retinas from RBEWT/WT and RBEKO/KO mice, respectively, for PSD95 (green, to label presynaptic photoreceptor terminals) and Cav1.4 (red, to label presynaptic Ca2+-channel). Scale bar: 20 µm; abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. (A1 to A4) Magnification of Cav1.4 staining in the outer plexiform layer (OPL) in the retina of RBEWT/WT and (B1 to B4) RBEKO/KO mice. White arrow heads indicate Ca2+-channel distribution at selected synapses. Images represent 2 µm z-stack projections. Scale bar: 2 µm."
NCBI gene IDs:
"RBE: "
Figure legend: "A. Transmission EM demonstrates the absence of synaptic ribbons in presynaptic terminals of photoreceptorribbon synapses in RBEKO/KOmice, while synaptic ribbons are readily visible in the terminals of RBEWT/WTmice. Asterisks in A2 and A3 denote photoreceptor active zones of RBEKO/KOmice in which the synaptic ribbon is absent. Except for the absence of synaptic ribbons, the ultrastructural appearance of the presynaptic terminals is comparable to that of RBEWT/WTmice. Abbreviations: sr, synaptic ribbon; pre, presynaptic; post, postsynaptic. Scale bars: 500 nm."
NCBI gene IDs:
"RBE: "
Figure legend: "B. Transmission EM demonstrates absence of synaptic ribbons in presynaptic terminals of bipolar cellribbon synapses in RBEKO/KOmice, while synaptic ribbons are readily visible in the terminals of RBEWT/WTmice. Except for the absence of synaptic ribbons, the ultrastructural appearance of the presynaptic terminals is comparable to RBEWT/WTmice. Abbreviations are the same as in A. Scale bars: 1 µm (B1, B2, B4, B5); 300 nm (B3, B6)."
NCBI gene IDs:
"RBE: "
Figure legend: "A and B. Representative transmission EM pictures of RBEWT/WT (A) and RBEKO/KO (B) photoreceptor ribbon synapses (blue rectangles, area adjacent to synaptic junction used for measuring junction-associated vesicle density [size = 200 nm x 300 nm]; black squares, are in the presynaptic cytosol used to measure cytosolic synaptic vesicles [size = 200 nm x 200 nm]).C. Average vesicle ratio (expressed as vesicle numbers in the area adjacent to synaptic junctions (blue rectangle) or the cytosol (black square) normalized to RBEWT/WT.D. Average number of docked vesicles within a 150 nm range in the vicinity of the active zone."
NCBI gene IDs:
"RBE: "
Figure legend: "A. Experimental design and representative traces for analysis of synaptic transmission in retinas from wild-type (black) and RIBEYE KO mice (red). Presynaptic bipolar neurons were depolarized in voltage-clamp mode from -70 mV to -10 mV for 0.5 s as indicated on top to open Ca2+-channels and trigger release. Presynaptic Ca2+-currents and postsynaptic EPSCs were measured simultaneously as indicated in the traces; dotted box displays an expansion of the initial Ca2+-currents and EPSCs.B. RIBEYE KO does not alter presynaptic Ca2+-currents. Summary graphs show average peak Ca2+-currents and Ca2+-current.C. RIBEYE KO severely impairs EPSCs triggered by presynaptic depolarization. Plot shows integrated EPSC charge as a function of time; both initial and sustained neurotransmitter release are impaired.D. RIBEYE KO strongly reduces initial synchronous release induced by presynaptic depolarization as indicated by the summary graph of the peak EPSC amplitude.E. RIBEYE KO suppresses both the initial synchronous and the sustained phase of release induced by presynaptic depolarization. Summary graphs display integrated EPSC charges for the initial phase (0-50 ms) and the sustained phase (50-500 ms).F. RIBEYE KO does not alter the kinetics of EPSCs. Summary graphs depict the mean synaptic delays (left), rise times (center) and decay time constants (right) of EPSCs."
NCBI gene IDs:
"RIBEYE: "
Figure legend: "A. Representative mEPSCs recorded from AII cells in wild-type (black) and RIBEYE KO mice (red). The insets in dashed boxes show mEPSCs on expanded scales, while the inset in a continuous box on the right shows the averaged mEPSC waveforms (average of 100-200 isolated mini events) of the example cells. Calibration bars apply to wild-type and mutant EPSCs.B. RIBEYE KO does not alter fundamental parameters of mEPSCs. Summary graphs show mean mEPSC frequency, amplitude, integrated charge, rise time, and decay time constant. Note that the only excitatory inputs into AII amacrine cells is provided by bipolar cell synapses."
NCBI gene IDs:
"RIBEYE: "
Figure legend: "C and D. RIBEYE KO renders mEPSCs sensitive to the slow Ca2+-buffer EGTA (C, representative mEPSCs traces recorded from AII cells after 30 minutes incubation in DMSO or EGTA-AM [0.2 mM]; D, summary graphs of the mean mEPSC frequency (left) and amplitude (right) after incubation in DMSO or EGTA-AM)."
NCBI gene IDs:
"RIBEYE: "
Figure legend: "Representative immunofluorescence  images of SCOTIN-KO cells expressing Str-Ii-SCOTIN-SBP-EGFP  with 40 μM D-biotin treatment for the indicated times. B Upper, localization  of RUSH reporters retained on the ER membrane with Sec61β  (red). Bottom, localization  of released RUSH reporters after 240 min of biotin  treatment. The arrow indicates the EGFP  signal on the ER. The arrowhead indicates the EGFP  signal on vesicles. The empty arrowhead indicates the EGFP  signal on the plasma membrane. Scale bar: 20 μm. Scale bars in the magnified images: 5 μm."
NCBI gene IDs:
"EGFP: 
SBP: 
SCOTIN: 51246"
Figure legend: "Representative immunofluorescence  images of SCOTIN-KO cells expressing Str-Ii-VSVG-SBP-EGFP  (green) with 40 μM D-biotin treatment for the indicated times. , Upper, localization  of RUSH reporters retained on the ER membrane with Sec61β  (red). Bottom, localization  of released RUSH reporters after 240 min of biotin  treatment. The arrow indicates the EGFP  signal on the ER. The arrowhead indicates the EGFP  signal on vesicles. The empty arrowhead indicates the EGFP  signal on the plasma membrane. Scale bar: 20 μm. Scale bars in the magnified images: 5 μm."
NCBI gene IDs:
"EGFP: 
SBP: 
SCOTIN: 51246
VSVG: 1489834"
Figure legend: "(A) Immunoblot  analysis of proteinase K protection assay. HeLa  cells were transfected with SCOTIN-Myc  (Total) and digitonin-permeabilized. The supernatants  (Cytosol) and pellet  (PNSs) were separated by centrifugation. The PNSs  were incubated with PBS  or with proteinase K (+ PK) in the presence or absence of  Triton X-100 (+PK  +Triton X)."
NCBI gene IDs:
"Myc: 
SCOTIN: 51246"
Figure legend: "(B) Representative high-resolution microscopy  images of Rab5  Q79L-EGFP- and SCOTIN-DsRed-expressing HeLa  cells. Magnified images show enlarged endosomes. Ⅰ: SCOTIN  on the endosomal membrane, Ⅱ: SCOTIN  on the endosomal membrane and luminal  side. Scale bar: 10 μm. Scale bars in the magnified images: 5 μm. (C) Plot analysis of SCOTIN  relative to the Rab5  Q79L signal shown. The graph indicates the fluorescence intensity of each channel on lines Ⅰ and Ⅱ from Figure 2B. The arrow indicates the boundary of the endosomal membrane."
NCBI gene IDs:
"DsRed: 
EGFP: 
Rab5: 5868
SCOTIN: 51246"
Figure legend: "(F) Left: Representative images of Rab5   Q79L-mCherry-expressing SCOTIN-FP11 knock-in cells. Right: Plot analysis of SCOTIN-FP11 relative to the Rab5    Q79L signal shown. The graph indicates the fluorescence intensity of each channel on the lines. The arrow indicates the boundary of the  endosomal membrane. Scale bars : 10 μm. Scale bars in the magnified images: 5 μm."
NCBI gene IDs:
"mCherry: 
Rab5: 5868
SCOTIN: 51246"
Figure legend: "(B) Representative images from a PLA  with VAPA   and Rab7   in WT and SCOTIN-Dsred-overexpressing HeLa  cells. Only one primary antibody (anti-Rab7  antibody alone) served as a negative control. The PLA  color legends are inverted. Asterisks indicate transfected cells. All scale bars: 20 μm. (C) The number of PLA  signals in each cell was quantified by particle analysis with ImageJ. Data information: n: number of cells analyzed. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, *&lt;0.05 and ****P&lt;0.0001."
NCBI gene IDs:
"Dsred: 
SCOTIN: 51246"
Figure legend: "(F) Immunoblot  analysis of WT and SCOTIN-KO HeLa  cell lysates for VAPA, Rab7, SCOTIN, and α-tubulin."
NCBI gene IDs:
"SCOTIN: 51246"
Figure legend: "(G) Representative images from a PLA  with VAPA  and Rab7  in SCOTIN-, TMPRD-, or PRD-Myc-transfected SCOTIN-KO HeLa  cells. The PLA  color legends are inverted. All scale bars: 20 μm. (H) The number of PLA  signals in each cell was quantified by particle analysis with ImageJ. Data information: n: number of cells analyzed. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, *&lt;0.05 and ****P&lt;0.0001."
NCBI gene IDs:
"Myc: 
SCOTIN: 51246"
Figure legend: "(B) Representative images of VAPA-Rab7 PLA  signals taken from Str-Ii-SCOTIN-SBP-EGFP  transfected SCOTIN-KO cells after untreated (-Biotin) or biotin  treatment (+ Biotin  4 h). For the PLA  signal, the color legends are inverted. Asterisks indicate transfected cells. All scale bars: 20 μm. (C) The PLA  signals in the indicated samples were quantified by particle analysis with ImageJ. Data information: n: number of cells analyzed. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, **&lt;0.01, ***&lt;0.001, and ****P&lt;0.0001."
NCBI gene IDs:
"EGFP: 
SBP: 
SCOTIN: 51246"
Figure legend: "(D) Representative images of Sec61β-Emerald- or Sec61β-PRD-Emerald-transfected SCOTIN-KO cells. Cells were stained  with RTN4  (red). Arrowheads indicate the accumulated ER membrane. All scale bars: 20 μm."
NCBI gene IDs:
"Emerald: 
Sec61β: 10952
SCOTIN: 51246"
Figure legend: "(C) HEK293  cells were transfected with SCOTIN-GST  along with empty (pcDNA3.1-Myc), SCOTIN-Myc, or SCOTIN(Δ150-177)-Myc  plasmids for 48 h. SCOTIN-GST  was  pulled down from total cell lysates using glutathione-Sepharose  beads, and the interacting  proteins were analyzed by immunoblotting."
NCBI gene IDs:
"Myc: 
SCOTIN: 51246"
Figure legend: "(D) Representative images from a PLA  with VAPA  and Rab7  in SCOTIN-KO HeLa cells reconstituted with FL SCOTIN, SCOTIN(ΔPRD), or SCOTIN(Δ150-177)-DsRed. The PLA  color legends are inverted. Asterisks indicate transfected cells. All scale bars: 20 μm."
NCBI gene IDs:
"DsRed: 
SCOTIN: 51246"
Figure legend: "(G) Representative images of VAPA-VAPA PLA  signals taken from Str-Ii-SCOTIN(Δ150-177)-SBP-EGFP  transfected SCOTIN-KO cells after untreated (-Biotin) or biotin-treated (+Bition  4 h). For the PLA  signal, the color legends are inverted. Asterisks indicate transfected cells. All scale bars: 20 μm. (H) The PLA  signals in the indicated samples were quantified by particle analysis with ImageJ. Data information: n: number of cells analyzed. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, ****P&lt;0.0001."
NCBI gene IDs:
"EGFP: 
SBP: 
SCOTIN: 51246"
Figure legend: "(A-D) TEM  images of the ER  and endosomes  of HeLa  (A), SCOTIN-KO HeLa  (B), SCOTIN-DsRed  transfected SCOTIN-KO HeLa  (C), and SCOTIN  Δ150-177-DsRed  transfected SCOTIN-KO HeLa  (D). DsRed-positive cells were processed for EM   quantification."
NCBI gene IDs:
"DsRed: 
SCOTIN: 51246"
Figure legend: "(A) Distribution of  late endosomes  (CD63) in WT or SCOTIN-KO HeLa  cells. Representative Z projections of confocal  images are shown. The color legends are inverted. Scale bars: 20 μm. (B) Spatial distribution of late endosomes  (CD63) expressed as the fractional distance of fluorescent pixels along a straight line drawn through the endolysosomal  cloud from the nuclear  center  (0) to the  plasma membrane (1.0) from the experimental data in Figure 8A. (C) Quantification of the number of CD63-positive vesicles  per cell from the experimental data in Figure 8A. Data information: n: number of cells analyzed, with # of data points analyzed per condition given in parentheses. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, ****P&lt;0.0001."
NCBI gene IDs:
"SCOTIN: 51246"
Figure legend: "(D) Live-image analysis of WT and SCOTIN-KO HeLa  cells expressing Rab7-EGFP. Left: confocal  images taken at time  0. Right: time-lapse  images of Rab7-EGFP  in WT and SCOTIN-KO HeLa  cells, as shown by temporal color-coded representations. Each confocal  image of late endosomes was color coded according to its acquisition time point, with images taken at 12-sec intervals over 5 min. A total of 24 color-coded images were projected to form a single image that demonstrates the dynamics of Rab7-labeled  late endosomes. White = static late endosomes. Multicolored = motile late endosomes. Boxed, magnified images highlight selected perinuclear  and peripheral  regions. Scale bar: 30 μm. Scale bars in the magnified images: 3 μm. (E) Quantification of the fractional distance of Rab7-EGFP-positive  late endosomes in WT and SCOTIN-KO HeLa  cells from the experimental data in Figure 8D. Error bars indicate the SEMs. (F) Quantification of Rab7-EGFP-positive   late endosome vesicles   per cell from the experimental data in Figure 8D. Data information: n: number of cells analyzed, with # of data points analyzed per condition given in parentheses. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, ****P&lt;0.0001."
NCBI gene IDs:
"SCOTIN: 51246"
Figure legend: "(G, H) Live-image analysis  of Rab7-EGFP  in SCOTIN-KO HeLa  cells reconstituted with SCOTIN-DsRed  (G) or SCOTIN(Δ150-177)-DsRed  (H) taken at time  0 or for 5 min (color-coded images). Lower, boxed, magnified images of selected perinuclear  and peripheral  regions. Scale bar: 20 μm. Scale bars in the magnified images: 2 μm. (I) Quantification of the fractional distance of Rab7-EGFP  in SCOTIN-KO cells reconstituted with SCOTIN-DsRed  or SCOTIN(Δ150-177)-DsRed  from the experimental data in Figure 8G and 8H. Data information: n: number of cells analyzed, with # of data points analyzed per condition given in parentheses. Data are shown as bars with dots and represent the means±SEMs. P values were determined using a two-tailed unpaired t test. Significant differences are labeled, ****P&lt;0.0001."
NCBI gene IDs:
"DsRed: 
SCOTIN: 51246"
Figure legend: "Representative images of seed  fluorescence segregation in 420/++ in wild type (Col) and hcr2. Scatterplots to the right show red (dsRed) and green (eGFP) fluorescence values in 420/++ Col (top) and hcr2  (bottom). Scale bars: 2 mm."
NCBI gene IDs:
"hcr2: 827261"
Figure legend: "End-point RT-PCR analysis of HSBP   in Col, hsbp-3, hsbp-2  and  hsbp-1. Hash and asterisk indicate aberrant long and short splicing variants of HSBP  in  hsbp-3, respectively. Image J was used to measure relative PCR  band intensity for hsbp-3 (53%), hsbp-2 (9%) and hsbp-1 (70%). TUB2  was used as an internal control. As in E, but showing immunoblot  analysis of HSBP. hsbp-3, hsbp-2 and hsbp-1 accumulate about 58%, 17% and 77% of HSBP  levels, respectively. Coomassie-stained  membrane was used as a loading control."
NCBI gene IDs:
"HSBP: 827261
hsbp: 827261
TUB2: 836390"
Figure legend: "Representative images of ASY1  (green) and RAD51  (red) immunostaining  in Col and hsbp-3. Nuclei  spreads were stained  with DAPI  (blue). Scale bars: 10 μm. Quantification of RAD51  foci numbers per cell in Col (blue) and hsbp-3 (red). n = 20 cells of biological replicates."
NCBI gene IDs:
"hsbp: 827261"
Figure legend: "Representative images of MLH1  (red) immunostaining  in Col, hsbp-3, hsbp-2, Col/Ler, and meiMIGS-HSBP  Col/Ler. Nuclear  DNA  was stained  with DAPI. Scale bar: 5 μm. Quantification of the number of MLH1  foci per cell shown in (G). n ≥ 32 cells of biological replicates."
NCBI gene IDs:
"HSBP: 827261
hsbp: 827261"
Figure legend: "Representative images of HEI10  (red) and ASY1  (green) immunostaining  in Col and hsbp-3. Scale bar: 2 μm."
NCBI gene IDs:
"hsbp: 827261"
Figure legend: "Immunoblot  analysis of HEI10  and HEI10-Myc in Col, hsbp-3, HEI10-myc  and HEI10-myc  plants. Experiments were performed at least three times."
NCBI gene IDs:
"myc: 
HEI10: 841784
hsbp: 827261"
Figure legend: "Summary of Hsp12-GFP dynamics single mutant screen. 68 mutants were divided into three groups: increased/decreased/did not change the total GFP produced. Each group was independently hierarchically clustered. The values shown are the Z-Scores of each parameter: ton- response onset time; production rate; total open time - length of transcriptional window (toff − ton); Basal GFP - GFP levels measured before exposure to stress; Delta GFP - amount of GFP produced throughout the experiment (Max GFP - Basal GFP)."
NCBI gene IDs:
"GFP: 
Hsp12: 850532"
Figure legend: "mRNA levels of HSP12 (Y axis) as a function of time (X axis) in six representative strains."
NCBI gene IDs:
"HSP12: 850532"
Figure legend: "Comparison of RNA levels to protein levels. X axis is the sum over the HSP12 mRNA counts (see D). Y axis is the maximum over the Hsp12-GFP values. The red line indicates the best linear fit."
NCBI gene IDs:
"HSP12: 850532"
Figure legend: "Illustration of the interactions of Δhog1 in the production rate parameter. Shown the production rate of the single mutant (X axis) versus the production rate of the double mutant with Δhog1 (Y axis). The gray line marks the Hog1 single mutant levels. Points close to the gray line are ones where the value of the double mutant is close to the value of Δhog1 and defined as epistasis of Δhog1 (pink dots)."
NCBI gene IDs:
"hog1: 850803
Hog1: 850803"
Figure legend: "Illustration of the interactions of Δhog1 for the total open time parameter."
NCBI gene IDs:
"hog1: 850803"
Figure legend: "Epistatic interactions of Δmck1 in the production rate parameter"
NCBI gene IDs:
"mck1: 855409"
Figure legend: "Epistatic interactions of Δmck1 in the total open time parameter"
NCBI gene IDs:
"mck1: 855409"
Figure legend: "Upper: HSP12 mRNA in response to osmotic stress (0.4M KCl). Lower: Estimate of Msn2/4 dependent production of HSP12 mRNA over time (difference between WT and Δmsn2Δmsn4 production profiles, Figure EV4A, Methods)."
NCBI gene IDs:
"HSP12: 850532
Msn2: 855053
msn2: 855053
msn4: 853803"
Figure legend: "   Δmck1 effect on the response of all stress genes. The integral over mRNA values in WT strain (X axis) vs. the integral in the Δmck1 strain (Y values). The inset shows the integral for the HSP12 gene.   "
NCBI gene IDs:
"HSP12: 850532
mck1: 855409"
Figure legend: "addition of the Δmsn5 strain."
NCBI gene IDs:
"msn5: 851935"
Figure legend: "C. LITATS1 expression upon SMAD4 knockdown (as detected by RT‒qPCR) in MDA-MB-231 cells. Cells were serum starved for 16 h and TGF-β was added for 4 h. Representative results from a minimum of three independent experiments are shown. D. LITATS1 expression (as detected by RT‒qPCR) in HEK293T cells. Cells were transfected without (Co.vec) or with the constitutively active TGF-β type I receptor (caTβRI) ectopic expression construct. Representative results from a minimum of three independent experiments are shown. E"
NCBI gene IDs:
"SMAD4: 4089
TGF-β type I receptor: 7046
TβRI: 7046
LITATS1: 728431"
Figure legend: "J. RNA fluorescence in situ hybridization was performed to evaluate LITATS1 expression and subcellular localization in A549 cells. Cells were treated with or without TGF-β for 2 h. Representative images are shown in the left panel, and signal quantification data are shown in the right panel. Scale bar=10 μm. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "A. LITATS1 expression in different breast cells as measured by RT‒qPCR. Results from epithelial-like and mesenchymal-like cells are labeled in blue and green, respectively. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "C. Quantification of LITATS1 expression levels by in situ hybridization in lung adenocarcinoma tissue microarrays. Representative images (bar=100 μm) and zoomed images (bar=20 μm) of in situ hybridization results in lung adenocarcinoma and matched adjacent normal tissues. The comparison of the LITATS1 staining index between the paired tissues is shown in the right panel. Tissue pairs with higher LITATS1 expression in the normal tissue (normal) than in the lung adenocarcinoma tissue (tumor) are highlighted in red, whereas tissue pairs with lower LITATS1 expression in the normal tissue than in the tumor tissue are highlighted in green."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "D. Kaplan‒Meier survival curves of relapse-free survival in 175 breast cancer patients stratified by LITATS1 expression. LITATS1 expression was measured by in situ hybridization in breast cancer tissue microarrays."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "Kaplan‒Meier survival curves of overall survival (E) in breast cancer patients stratified by LITATS1 expression. The data were generated via Kaplan‒Meier Plotter"
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "Kaplan‒Meier survival curves of distant metastasis-free survival (F) and relapse-free survival (G) in breast cancer patients and overall survival (H) in non-small cell lung cancer patients stratified by LITATS1 expression. The data were generated via Kaplan‒Meier Plotter"
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "Effect of LITATS1 on TGF-β-induced EMT marker expression in MCF10A-M2 upon CRISPRa-mediated LITATS1 overexpression (A) GAPDH loading control. Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"CRISPR: 
LITATS1: 728431"
Figure legend: "Effect of LITATS1 on TGF-β-induced EMT marker expression in MCF10A-M2 upon shRNA-mediated knockdown (B). α/β-Tubulin, loading control. The results of LITATS1 overexpression Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "D, E. An IncuCyte chemotactic migration assay was performed to evaluate the effect of LITATS1 ectopic expression (D) or knockdown (E) on TGF-β-induced MDA-MB-231 cell migration. The results of LITATS1 overexpression and E. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "F, G. In vivo zebrafish extravasation experiments with MDA-MB-231 cells upon ectopic LITATS1 expression (F) or LITATS1 knockdown (G). Representative zoomed images of the tail fin area are shown in the left panels. Extravasated breast cancer cell clusters are indicated with yellow arrows. Analysis of the extravasated cell cluster numbers in the indicated groups is shown in the right panel. Whole zebrafish image, bar=309.4 μm; zoomed image, bar=154.7 μm. Representative results from two independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"
Figure legend: "C. Expression of TGF-β target genes (as measured by RT‒qPCR) in MDA-MB-231 cells without (Co.sh) or with (sh#1 and sh#2) LITATS1 depletion. Cells were serum starved for 16 h and treated with or without TGF-β for 4 h. Representative results from a minimum of three independent experiments are shown."
NCBI gene IDs:
"LITATS1: 728431"