EMBO/SourceData huggingface dataset:
Figure legend: "(B). USP36 is required for rRNA processing. HeLa cells transfected with scr or USP36 siRNA (left panels) or infected with scr or USP36 shRNA lentiviruses (right panels) were assayed for rRNA processing by Northern blot using 5'- ETS, ITS1 and ITS2 probes as indicated."
NCBI gene IDs:
"USP36: 57602"
Figure legend: "Knockdown of USP36 inhibits translation. HeLa cells infected with scr or USP36 shRNA lentiviruses were incubated with O-propargyl-puromycin (OPP) followed by Click-iT OPP protein synthesis assays. Representative images (C) and quantification from three independent experiments (D). Data were presented as mean ± SD, n=3 biological replicates. ***P&lt;0.001, compared to scr control (Student's t-test). Scale bar = 50 μM."
NCBI gene IDs:
"USP36: 57602"
Figure legend: "(A) DIV 3 cortical neurons immunolabelled for PIP3. Spectrum heatmap shows fluorescence intensity. (B) DIV 8 cortical neuron transfected with GFP and immunolabelled for PIP3. Spectrum heatmap shows fluorescence intensity. (C) DIV 16 cortical neuron transfected GFP and immunolabelled for PIP3. Spectrum heatmap shows fluorescence intensity. (D) Somatic PIP3 quantification in the soma at increasing days in vitro. Data are shown as the mean +/- SEM. P values are significance measured by ANOVA with Tukey's post-hoc analysis. N=3 experiments, 60 neurons. (E) Growth cone PIP3 quantification at increasing days in vitro. Data are shown as the mean +/- SEM. P values indicate significance measured by ANOVA with Tukey's post-hoc analysis. N=3 experiments, 60 growth cones. "
NCBI gene IDs:
"GFP: "
Figure legend: "(A) PIP3 immunofluorescence in the soma of DIV 16 cortical neurons expressing p110 isoforms and GFP. Spectrum heatmap shows fluorescence intensity. (B) PIP3 immunofluorescence at the axonal growth cone of DIV 16 neurons expressing p110 isoforms and GFP. Spectrum heatmap shows fluorescence intensity. (C) Quantification of PIP3 immunofluorescence in the soma. Data are shown as the mean +/- SEM. P values indicate significance as measured by ANOVA with Tukey's post-hoc analysis. N=3 experiments, 60 neurons. (D) Graph showing PIP3 quantification of immunofluorescence at the axon growth cone. Data are shown as the mean +/- SEM. P values indicate significance measured by ANOVA with Tukey's post-hoc analysis. N=3 experiments, 60 growth cones. "
NCBI gene IDs:
"GFP: 
GFP.: 
p110: 5293///5290"
Figure legend: "(A) DIV 4 cortical neurons expressing p110 isoforms and GFP. Arrow marks the axon tip. (B) Quantification of axon length. n = 3 experiments, 45 neurons per group. (C) Quantification of dendrite length. n = 3 experiments, 45 neurons per group. (D) Quantification of the axon:dendrite length ratio. n = 3 experiments, 45 neurons per group. "
NCBI gene IDs:
"GFP: 
p110: 5293///5290"
Figure legend: "(E) DIV 14 cortical neurons expressing p110 isoforms and GFP, immunolabelled for pS6. (F) Sholl analysis of branches. n = 60 neurons per group. Data are shown as the mean +/- SEM. P values indicate significance measured by repeated measure ANOVA with Bonferonni's post-hoc test. (G) Quantification of the total neurite length. n = 3 experiments, 60 neurons per group. (H) Quantification of soma area. n = 3 experiments, 60 neurons per group. (I) Quantification of the pS6 immunofluorescence. n = 3 experiments, 60 neurons per group. "
NCBI gene IDs:
"GFP: 
p110: 5293///5290"
Figure legend: "(A) Axotomised DIV 14-16 rat cortical neurons expressing p110δ and GFP or GFP alone. Axons were cut &gt;1000 μm from the soma imaged for 14 h. Black arrows mark the cut site, white arrows marks the axon tip. See also Movies EV7 and EV8. (B) Percentage of regenerating axons 14 h after laser axotomy. Numbers on bars are injured axons per group, from 4 experiments. (C) Quantification of axon regeneration length 14 h post-axotomy. n=9 axons for GFP, 9 for p110α, 25 for p110αH1047R and 27 for p110δ from 4 experiments. (D) Quantification of time to start of regeneration. n=9 axons for GFP, 9 for p110α, 25 for p110αH1047R and 27 for p110δ from 4 experiments. "
NCBI gene IDs:
"GFP: 
p110α: 5290
p110δ: 5293"
Figure legend: "(E) Axotomised human embryonic stem cell neurons p110δ and GFP or GFP alone. Black arrow marks the cut site, white arrows mark the axon tip. (F) Percentage of regenerating axons of human hESC neurons, Numbers on bars are injured axons per group, from 5 experiments. (G) Quantification of axon regeneration length. n=22 axons for GFP, 44 for p110δ, from 5 experiments. Data are shown as the mean +/- SEM. Data were analysed by two tailed Student's T-test. (H) Quantification of time to start of regeneration. n=22 axons for GFP, 44 for p110δ, from 5 experiments. Data are shown as the mean +/- SEM. Data were analysed by two tailed Student's T-test. "
NCBI gene IDs:
"GFP: 
p110δ: 5293"
Figure legend: "(A) Percentage of regenerating axons after laser axotomy of DIV 14-17 rat cortical neurons expressing either GFP (control) or GFP and p110δ in the presence of the indicated inhibitors, 14h after laser injury. Numbers on bars are injured axons per group."
NCBI gene IDs:
"GFP: 
p110δ: 5293"
Figure legend: "(B) Single confocal section through a DIV 16 neuron expressing p110δ and GFP (upper panels). Green colour is GFP, red is overexpressed p110δ, detected with anti-p110δ. Lower panels show p110δ and GFP in a distal axon section, and at the growth cone. Arrow indicates the axon."
NCBI gene IDs:
"GFP: 
p110δ: 5293"
Figure legend: "(C) Active ARF and total ARF6 (red) in axons of DIV 16 rat cortical neurons expressing GFP or GFP and p110δ, as indicated. Arrows indicate axons. (D) Quantification of mean axonal ARF activity and total (mean) ARF6 in DIV 16 neurons expressing GFP or GFP and p110δ n=60 axons for each condition. "
NCBI gene IDs:
"GFP: 
p110δ: 5293"
Figure legend: "(E) Kymographs showing dynamics of α9 integrin-GFP in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. (F) Quantification of α9 integrin-GFP dynamics in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. n numbers are axon sections analysed for each condition. (G) Quantification of total α9 integrin-GFP number in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. n=43 for control, 47 for co-transfected with p110δ. n numbers are axon sections analysed for each condition."
NCBI gene IDs:
"GFP: 
α9 integrin: 3680
p110δ: 5293"
Figure legend: "(H) Kymographs showing dynamics of Rab11-GFP in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. (I) Quantification of Rab11-GFP dynamics in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. n numbers are axon sections analysed for each condition. (J) Quantification of total Rab11-GFP in the distal axons of DIV 14-16 neurons, control or co-transfected with p110δ. n=43 for control, 53 for co-transfected with p110δ. n numbers are axon sections analysed for each condition. "
NCBI gene IDs:
"GFP: 
p110δ: 5293
Rab11: 8766"
Figure legend: "(D) Percentage of phospho-S6 positive cells in the RGC layer 2 weeks after delivery of AAV-Cre-GFP or AAV-GFP."
NCBI gene IDs:
"GFP: 
Cre: 2777477"
Figure legend: "G) Representative images of CTB-labelled RGC axons 4 weeks after optic nerve crush in wild type (c57bl/6), Rosa26-p110αH1047R, and Rosa26-p110δ transgenic mice injected with AAV2.Cre.GFP. Dashed line indicates crush site."
NCBI gene IDs:
"GFP: 
Rosa26: 
Cre: 2777477
p110α: 5290
p110δ: 5293"
Figure legend: "(A) Retinal sections from mice injected with AAV2 viruses as indicated, immunolabeled for phospho-S6. Blue colour is DAPI to indicate nuclei. Inset images show individual colours at the same scale as the full image. (B) Percentage of phospho S6 positive cells in the retinal ganglion layer of mice injected with AAV2.shScramble.GFP or AAV2.shPTEN.GFP. (C) Number of pS6-postive RGCs of mice injected with AAV2.GFP or AAV2.p110δ."
NCBI gene IDs:
"GFP: 
p110δ: 5293
PTEN: 19211"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I) or control followed by western blot analysis (A) The basal levels of p53 normalized to GAPDH were arbitrarily set at 1.0 and the fold change is shown in (ii) (n=4)."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 or control (A-I) combined or not with a treatment with doxorubicin (Doxo) (positive control for phosphorylation of ATM and p53) (B) followed by western blot analysis."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), ASF (C) (positive control for y-H2AX activation [29])or control (A-I) followed by western blot analysis."
NCBI gene IDs:
"ASF: 6426
Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), or control (A-I) combined or not with a treatment with wortmannin (WTN) (D) followed by western blot analysis."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), or control (A-I) combined or not with a treatment with NU-7441 (E,F) followed by western blot analysis."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), XRCC4 (F) or control (A-I) combined or not with a treatment with NU-7441 (E,F), followed by western blot analysis."
NCBI gene IDs:
"XRCC4: 7518
Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), or control (A-I) followed by RT-qPCR analysis (G). In RT-qPCR analysis (G), mRNA levels were standardized against HPRT mRNA (n=3). The two panels of Fig. 1C are partion of the same gel. Statistical analysis by unpaired t-test (*P&lt;0.05; **P&lt;0.01). All error bars reflect SEM."
NCBI gene IDs:
"HPRT: 
Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), or control (A-I) combined or not with a treatment with MG132 (H) followed by western blot analysis (A-F, H-I)."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "Treatment of HCT116 cells with siRNA targeting Ku70 (A-I), or control (A-I) combined or not with a treatment with cycloheximide (CHX) (I) followed by western blot analysis (A-F, H-I)."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "(A) RNA affinity chromatography using the p53 5'UTR (5'p53) or a portion of the p47 5'UTR (5'p47) (depicted in (i)) and HeLa cytoplasmic extracts (CE), followed by western blot analysis (ii)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(B) IP of in cellulo RNA-protein complexes (RIP) in HCT116 cells, followed either by RT-qPCR analysis. Bar plots represent the mean and the data of two independent experiments (i) or by western blot analysis (ii). The relative p53 mRNA levels for each IP sample were normalized to the corresponding IP IgG and to the corresponding input sample and were plotted relatively to the HPRT mRNA."
NCBI gene IDs:
"HPRT: 
p53: 7157"
Figure legend: "(C) UV cross-linking of recombinant (Ku70/Ku80) with the in vitro transcribed 5'p53 with alpha 32P-GTP or alpha 32P-UTP (i). UV cross-linking of recombinant Ku70/Ku80 with the in vitro transcribed 5'p53 and the Ctrl (5'p53 antisense) with alpha 32PGTP (ii)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(A) Non polysomal (NP) and polysomal (P) fractions were extracted from HCT116 cells transfected with the siRNA Ku70 and quantitative RT-qPCR was performed using specific primers for p53 and HPRT mRNAs. The p53 mRNA levels in P and NP fractions were normalized to the input (n=3)"
NCBI gene IDs:
"HPRT: 3251
p53: 7157
Ku70: 2547"
Figure legend: "(B) Western blot from H1299 (p53-null) cells treated with siRNA Ku70 or control (ii), followed by transfections of RNA constructs depicted in (i). p53 protein levels from the WT and the ΔB2 reporters depicted in (i) normalized to firefly luciferase (transfection control reporter) (iii, n=3)."
NCBI gene IDs:
"firefly: 
p53: 7157
Ku70: 2547"
Figure legend: "(D) RT-qPCR analysis of Bax, TP53I3, BTG2, GADD45α and p21 after siRNA-mediated Ku or control depletion in HCT116p53+/+ or HCT116p53-/- (n=3). Relative mRNA levels were standardized against GAPDH mRNA. Statistical analysis by unpaired t-test (*P&lt;0.05; **P&lt;0.01; *** P&lt;0.001 NS, non significant). All error bars reflect SEM."
NCBI gene IDs:
"GAPDH: 
Bax: 581
BTG2: 7832
p21: 1026
GADD45α: 1647
p53: 7157
TP53I3: 9540
Ku: 7520///2547"
Figure legend: "(A) Western blot analysis after treatment with Ku70 or control siRNA, followed by incubation with DMSO, etoposide (ETO) or bleomycin (BLEO) for 16 h (i). p53 levels after Ku70 depletion normalized to siRNA control for each condition (ii, n=3). All the experiments were performed with HCT116 cells. GAPDH: loading control in A, B, C. Statistical analysis by unpaired t-test (*P&lt;0.05; **P&lt;0.01; *** P&lt;0.001; NS, non significant). All error bars reflect SEM."
NCBI gene IDs:
"Ku70: 2547"
Figure legend: "(B) Western blot analysis as in A, except that tricostatin A (TSA) was used after Ku/control knockdown (n=4). All the experiments were performed with HCT116 cells. GAPDH: loading control in A, B, C. Statistical analysis by unpaired t-test (*P&lt;0.05; **P&lt;0.01; *** P&lt;0.001; NS, non significant). All error bars reflect SEM."
NCBI gene IDs:
"Ku: 2547"
Figure legend: "(D) RNA affinity chromatography using the 5'p53RNA and total extracts from cells treated with TSA, ETO or BLEO for 24 h, followed by western blot analysis of Ku70 quantified and normalized to the input (ii, n=3). All the experiments were performed with HCT116 cells. Statistical analysis by unpaired t-test (*P&lt;0.05; **P&lt;0.01; *** P&lt;0.001; NS, non significant). All error bars reflect SEM."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(E) RNA affinity chromatography using the 5'p53RNA and total extracts from cells transfected or not with PCAF encoding plasmid for 48 h, followed by western blot analysis. All the experiments were performed with HCT116 cells."
NCBI gene IDs:
"PCAF: 8850
p53: 7157"
Figure legend: "(F) RNA affinity chromatography using the 5'p53RNA or a dsDNA and total extracts from cells transfected with HA-tagged Ku70 wild-type (WT) or mutated (Mut6E) Ku70 [25] for 48 h, followed by western blot analysis. All the experiments were performed with HCT116 cells."
NCBI gene IDs:
"p53: 7157
Ku70: 2547"
Figure legend: "(G) RNA affinity chromatography as in F, but with two additional Ku mutants (K282/338Q and K282/338R), followed by western blot of Ku70 quantified and normalized to the input and to the loading control, PTB (ii, n=3) All the experiments were performed with HCT116 cells."
NCBI gene IDs:
"Ku: 2547
Ku70: 2547"
Figure legend: "F. Segregation of c.2241C&gt; G, p.Y747X in PPP1R13L in the studied family: The affected girl VI10, is homozygous for the causative SV p.Y747X, as are the four fetuses that were aborted. The parents are obligate carriers as shown and, of the two healthy sisters, one carries the wild allele only, and the other is heterozygous for the causative SV."
NCBI gene IDs:
"PPP1R13L: 10848"
Figure legend: "A. PPP1R13L mRNA expression level is lower in VI10 PDFs (set as 1) than in CFs."
NCBI gene IDs:
"PPP1R13L: 10848"
Figure legend: "D. WB analysis demonstrated that PPP1R13L-shRNA downregulated the putative iASPP band."
NCBI gene IDs:
"PPP1R13L: 10848"
Figure legend: "C. knockdown of PPP1R13L in CFs resulted in increased mRNA expression levels of inflammatory mediators. Differences between knockdown and control with p values (Student's t test) are indicated with asterisks in the graph."
NCBI gene IDs:
"PPP1R13L: 10848"
Figure legend: "B. ChIP assay shows that p65 is associated more strongly with inflammatory cytokine gene promoters in LPS-stimulated PDFs than in LPS-stimulated-CFs. The binding activity at the IL1B promoter was set as 1. Differences between knockdown and control with p value= 0.00436 (Student's t test) are indicated with an asterisk."
NCBI gene IDs:
"IL1B: 3553"
Figure legend: "A. WB analysis confirmed the reduced expression of iASPP in ppp1r13l knocked down cardiomyocytes."
NCBI gene IDs:
"ppp1r13l: 333654"
Figure legend: "B. qPCR showed that ppp1r13l-knocked down murine cardiomyocytes (using two alternative shRNA sequences) expressed higher level of pro-inflammatory cytokine mRNAs after 4 hrs of LPS stimulation. The expression in ppp1r13l-knocked down murine cardiomyocytes was set as 1. Genome wide expression analysis is presented in Figure 1S. Differences between knockdown and control with p values (Student's t test) are indicated with asterisks and exact values."
NCBI gene IDs:
"ppp1r13l: 333654"
Figure legend: "A wa3 hearts express higher level of Il1 than littermate WT hearts without significant changes in selected structural and regulatory genes as myosin binding protein C (Mybpc3), phospholamban (Pln), ryanodine receptor 2 (Ryr2), and sarcoglycan gamma (Sgcg). Hearts removed from perfused mice and mRNA was subjected for q PCR. Difference between knockdown and control with p values = 0.03 (Student's t test) is indicated with an asterisk."
NCBI gene IDs:
"Mybpc3: 17868
Pln: 18821
Ryr2: 20191
Sgcg: 24053"
Figure legend: "B. A scatter plot showing the deviation from normal expression by highlighting the differentially expressed genes of newborn, 7-week- and 12-week- hearts (p&lt;0.05 threshold, calculated by Deseq algorithm \"R\"). The expression level of s100a9 was higher in wa3 mice than in WT by 2.8 times. For the full list of genes see Tables S1 and S2, and functional analyses in Figures S3-S6."
NCBI gene IDs:
"s100a9: 20202"
Figure legend: "A. LPS (5mg/Kg for 2 hrs) induced higher expression levels of cytokine genes in wa3 hearts than in littermate WT hearts. The results are presented as fold induction of untreated controls. The mRNA expression levels of Pln and Ryr2 was reduced in both wa3 and WT hearts, while of Mybpc3 and Sgcg was unchanged. For the full list see Dataset EV3 and for functional clustering in Appendix Figure S7. Differences between knockdown and control with p values =0.04998 (Student's t test) are indicated with an asterisk."
NCBI gene IDs:
"Mybpc3: 17868
Pln: 18821
Ryr2: 20191
Sgcg: 24053"
Figure legend: "E. Influence of cy3 label on the 5' and 3' end of oligo#5. Using a phosphate linker, Cy3 was added to the 5' or 3' phosphate, respectively and the oligonucleotides assayed by RT-PCR. Oligo#5 with cy3 at the 5' end showed 86% inclusion, with Cy3 at the 3' end 17% exon inclusion.F. Statistical analysis of the effect of oligonucleotide modification. The effect is significant, p=0.009, n=4."
NCBI gene IDs:
"cy3: 
Cy3: "
Figure legend: "C. Effect of oligo#5 on the truncated 5HT2C protein. Stable cell lines expressing the construct shown in panel (A) were treated with oligo#3 and oligo#5. After 48 hrs cells were separated in membrane containing fractions and soluble cytosolic supernatant. The fractions were analyzed by Western Blot using the anti-RNA1 antiserum (Figure EV1). The protein samples were on the same membrane, but the supernatant fractions were exposed about ten times longer.D. Effect of oligo#5 on the full-length 5HT2C protein. The protein was prepared as in panel (C) and analyzed using a polyclonal anti-GFP antiserum.E. Quantification of panels C and D from three independent experiments. The ratio of protein signal after oligo#3 and oligo#5 treatment is shown for RNA1 (p=0.002) and RNA2 (p=0.0003), n=3, respectively."
NCBI gene IDs:
"5HT2C: 3358"
Figure legend: "G. Effect of oligo#5 on POMC expression. The RNA from panel D was analyzed using qPCR with primers against POMC, normalized to GAPDH (3 hrs: p=0.0009; 6 hrs: p=0.02; 9 hrs: p=0.1; 12 hrs: p=0.3, n=3)."
NCBI gene IDs:
"GAPDH: 
POMC: 18976"
Figure legend: "H. Effect of oligo#5 on food uptake in wild-type mice. C57BL/6 wild-type mice received a guide canulae and did not have access to food for 16 hours. After injection of 2 µg oligonucleotides, they could freely access food. The food consumption was measured by weighing the food used. A total of 52 animals were used, p=0.001 for the difference between oligo#5 and control oligonucleotides. Each of the six individual experiments showed statistically significant differences between oligo#5 and control (p&lt;0.01 - 0.001). Control oligo: an oligonucleotide against human SMN2."
NCBI gene IDs:
"SMN2: 6607"
Figure legend: "I. Effect of oligo#5 on food uptake in ob/ob mice. Ob/obmice received oligo#5 through ICV injection, similar to panel F. However, the mice could freely access food at all times (p=0.04, n=3)."
NCBI gene IDs:
"ob: 16846"
Figure legend: "(A) Western blot analysis of the immunoproteasome subunits LMP2, LMP7 and β-actin (loading control) in three PolgA+/+ and three PolgAmut/mut cell lines."
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(B) RT-qPCR analysis of immunoproteasome subunits Psmb8, Psmb9, Psmb10 in three different PolgA+/+ and three PolgAmut/mut cell lines."
NCBI gene IDs:
"PolgA: 18975
Psmb10: 19171
Psmb8: 16913
Psmb9: 16912"
Figure legend: "(C) Activity assay for the catalytic subunits of the proteasome using the pan activity-based probe (ABP) MV151, the β1/LMP2 specific LW124, and the β5/LMP7 specific MVB127 ABPs in three PolgA+/+ and three PolgAmut/mut cell lines. Resolution of β5/LMP7 in the SDS gel is hampered as both subunits are of similar molecular weight and thus do not separate in our gels. Total protein staining was used as a loading control."
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(D) representative Western blots for the analysis of the cGAS/STING signaling pathway in PolgA+/+ and PolgAmut/mut cells with β-actin serving as loading control."
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(E) Secreted IFNβ in supernatants from three distinct PolgA+/+ and PolgAmut/mut cell lines. The 15.81 pg/ml detected by ELISA in PolgAmut/mut cell supernatants corresponds to 18.97 IU/ml recombinant mouse (rm) IFN-β according to the R&amp;D Systems cytokine conversion table"
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(F) RT-qPCR analysis of Stat1 in three different PolgA+/+ and three PolgAmut/mut cell lines."
NCBI gene IDs:
"PolgA: 18975
Stat1: 20846"
Figure legend: "(A) Western blot analysis of LMP2, LMP7, STAT1 and β-actin (loading control) in PolgA+/+ cells after incubation with either PolgA+/+ or PolgAmut/mut cell-conditioned medium for 48 h. Densitometric analysis of LMP2, LMP7 and STAT1 expression normalized to β-actin loading control set to 1 in PolgA+/+ incubated with either PolgA+/+ or PolgAmut/mut cell-conditioned medium from three different cell lines."
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(B) Western blot analysis of LMP2, LMP7, STAT1, and β-actin (loading control) upon siRNA-mediated silencing of cGAS or STING in PolgAmut/mut cells compared to untransfected (Ctrl) and non-sense scrambled transfection control (NC) in three independent experiments."
NCBI gene IDs:
"cGAS: 214763
PolgA: 18975
STING: 72512"
Figure legend: "(E) Western blot analysis of LMP2, LMP7, STAT1, and β-actin (loading control) in PolgA+/+ cells transfected with mouse wildtype mtDNA, mtDNA pretreated with DNase I or only DNase I for 48 h. Densitometric analysis of LMP2, LMP7 and STAT1 expression normalized to β-actin loading control in PolgA+/+ cells. Values are given as fold over PolgA+/+ control with the mean of normalized PolgA+/+ signal normalized to 1. (F) Western blot analysis of LMP2, LMP7, STAT1 and β-actin (loading control) in PolgA+/+ cells transfected with cGAMP for 24 h. Densitometric analysis of LMP2, LMP7 and STAT1 expression normalized to β-actin loading control (Ctrl) set to 1."
NCBI gene IDs:
"PolgA: 18975
cGAMP: 72512"
Figure legend: "(G) Protein expression of LMP2, LMP7 and β-actin (loading control) in wildtype (WT), cGAS KO or STINGgt/gt losss of function MEFs that had been transfected with 200 ng/ml mtDNA for 48 h."
NCBI gene IDs:
"cGAS: 214763
STING: 72512"
Figure legend: "(B) UTY-assay of CD8+ reporter T cells upon co-culture with different ratios of PolgA+/+ or PolgAmut/mut cells. Data are combined results of three independent experiments and normalized to the signal of maximum induction of PolgA+/+ cells in co-culture with CD8+ reporter T cells."
NCBI gene IDs:
"PolgA: 18975"
Figure legend: "(E) UTY-assay of CD8+ reporter T cells upon 1:2 co-culture (splenocytes:T cells) with splenocytes isolated from either WT or LMP7 KO mice and transfected with mtDNA. Splenocytes were isolated from 4 mice each in two different preparations."
NCBI gene IDs:
"LMP7: 16913"