EMBO/SourceData huggingface dataset:
Figure legend: "(D) Colony formation ability after deacetylation of IDH1 K224 or knockout of IDH1. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For statistical significance was assessed with a one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(E) Cell cycle distribution was assessed flow cytometrically after treatment with IDH1 K224R or knockout of IDH1. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For E, statistical significance was assessed with a one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(A) Murine Ntras  enrichment in endothelial cells versus other heart-derived cell types (data acquired from GSE95755 (n = 4 mice). Data information: In (A, data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. (A) One-way ANOVA"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(B) Changes in lncRNA expression  from HUVECs  exposed to hypoxia   (0.2 %  O2 for 12 h or 24 h), determined by ribo-minus RNA deep sequencing  (data acquired from GSE107033 ; NTRAS  is highlighted (n = 2 independent biological replicates)."
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(C) Validation of hypoxia-induced NTRAS expression  at the indicated time points by RT-qPCR  (n = 4 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. two-tailed unpaired t-test"
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(D) Subcellular localization  of NTRAS  and control transcripts, assayed by nuclear-cytoplasmic  fractionation of HUVECs and RT-qPCR (n = 3 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. two-tailed unpaired t-test"
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(E) FITC-dextran-based in vitro permeability  comparing control and NTRAS-silenced HUVECs  (n = 5 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. two-tailed unpaired t-test,"
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(F) Cumulative in vitro sprout  lengths of control and NTRAS-silenced HUVECs  under basal conditions and VEGFA  stimulation (n = 3-10 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001."
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(G) Cell cycle analysis in Ntras-silenced H5V cells (n = 3 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. (G) two-way ANOVA,"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(H) TMR-dextran-based assessment of vascular  permeability in vivo, comparing heart  homogenates from control and Ntras-silenced mice. Data normalized to organ and body weight (n = 11-12 mice  per group). Experimental outline on the left. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001. H) two-tailed unpaired t-test"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(I) Survival  of control and Ntras-silenced mice under basal conditions (black, n = 21-23 mice  per group) and in hind limb ischemia (HLI; red, n = 8 mice  per group). Number of survivors is indicated; experimental outline on the left. Data information: (I) Mantel-Cox-test."
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(A) Separation of mock and proteinase K-treated HeLa nuclear  extracts by  sucrose  density gradient ultracentrifugation followed by NTRAS  detection using  RT-qPCR. Fractions 1 and 14 represent top and bottom of the gradient, respectively (n = 1)."
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(B) Enrichment of NTRAS  by  antisense affinity selection  from HeLa  cell lysate followed by  RT-qPCR, comparing a control and an NTRAS-specific probe (n = 5 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. two-tailed unpaired t-test."
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(D) RT-qPCR-based validation of NTRAS-hnRNPL-interaction  by anti-hnRNPL RIPs  from HUVEC nuclear  fractions (n = 6 independent biological replicates). Representative  western blot  on the right. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. two-tailed unpaired t-test."
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(E)  RT-qPCR analysis of NTRAS expression   in HUVECs  upon silencing of hnRNPL (n = 4 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. two-tailed unpaired t-test."
NCBI gene IDs:
"hnRNPL: 3191
NTRAS: 257194"
Figure legend: "(F) RPLP0-normalized NTRAS-CA16 motif expression  after hnRNPL  silencing determined by  RT-PCR (n = 4 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. two-tailed unpaired t-test."
NCBI gene IDs:
"hnRNPL: 3191
NTRAS: 257194
RPLP0: 6175"
Figure legend: "(H) NTRAS  and hnRNPL  co-regulated alternative splicing  (AS) events. Data points with an FDR &lt; 0.05 are circled (n = 2 independent biological replicates)."
NCBI gene IDs:
"hnRNPL: 3191
NTRAS: 257194"
Figure legend: "(I) RT-PCR-based analysis of  TJP1 exon 20 inclusion  upon silencing of NTRAS  in HUVECs  (n = 7 independent biological replicates). Representative agarose  gels on the right. Data information: , I, data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. I, two-tailed unpaired t-test."
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(J) RT-PCR-based analysis of TJP1 exon 20 inclusion upon silencing of hnRNPL  in HUVECs  (n = 4 independent biological replicates). Representative agarose  gels on the right. Data information: , J) data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, ***p &lt; 0.001. J) two-tailed unpaired t-test."
NCBI gene IDs:
"hnRNPL: 3191
TJP1: 7082"
Figure legend: "(B) In vitro splicing  efficiency of the TJP1  splice substrate, comparing mock, NTRAS-depleted, and NTRAS-CA16 motif add-back conditions (n = 7-12 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. (B two-tailed unpaired t-test,"
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(C) Co-precipitation of TJP1  pre-mRNA in anti-hnRNPL RIPs, using nuclear  lysates from control and NTRAS-silenced HUVECs  (n = 6 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed paired t-test"
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(D) Co-precipitation of TJP1  pre-mRNA in anti-hnRNPL RIPs, using nuclear  lysates from control and NTRAS-overexpressing cells (n = 5 independent biological replicates). Representative western blot  on the right. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed paired t-test,"
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(E) Co-precipitation of TJP1  pre-mRNA in anti-hnRNPL RIPs, using nuclear  lysates from control and NTRAS-CA16 motif overexpressing cells (n = 5 independent biological replicates). Representative western blot on the right. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed paired t-test,"
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(F) RT-PCR-based analysis of TJP1 exon 20 inclusion upon NTRAS  overexpression and overexpression of the NTRAS-CA16 motif (n = 8 independent biological replicates). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. one-way ANOVA."
NCBI gene IDs:
"NTRAS: 257194
TJP1: 7082"
Figure legend: "(G) Endothelial   resistance of NTRAS-silenced HUVECs  (n = 3-5 independent biological replicates), analyzed by electrical cell-substrate impedance sensing (ECIS). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed unpaired t-test"
NCBI gene IDs:
"NTRAS: 257194"
Figure legend: "(H) Endothelial  resistance of hnRNPL-silenced HUVECs  (n = 3 independent biological replicates), analyzed by ECIS. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed unpaired t-test,"
NCBI gene IDs:
"hnRNPL: 3191"
Figure legend: "(I) Analysis of TJP1 exon 20 inclusion by RT-PCR  upon transfection of HUVECs  with a control SSO or an SSO masking the exon 20 - intron 20 boundary (E20  SSO) (n = 9 independent biological replicates). Representative agarose  gel on the right. Schematic outline at the top right. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01. two-tailed unpaired t-test,"
NCBI gene IDs:
"TJP1: 7082"
Figure legend: "(A) Validation of Ntras-hnRNPL-interaction  by anti-hnRNPL RIPs  from H5V lysates, followed by RT-qPCR  (n = 4 independent biological replicates). Representative western blot on the right. Data information: In (A, data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. (A) two-tailed paired t-test"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(B)  RT-PCR-based analysis of murine Tjp1 exon 20 inclusion in cardiac  tissue from control and Ntras-silenced mice  (n = 8 mice  per group). Experimental outline on the left. Data information: In data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. (B, two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840
Tjp1: 21872"
Figure legend: "(D) Co-precipitation of Ntras  in anti-hnRNPL RIPs  using whole heart  lysates from NtrasCA/CA and Ntras∆CA/∆CA mice  (n = 3-4 mice  per group). Representative  western blot  on the right. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(E) RT-PCR-based analysis of TJP1 exon 20 inclusion in hearts  from NtrasCA/CA and Ntras∆CA/∆CA mice  (n = 9-14 mice  per group). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840
TJP1: 21872"
Figure legend: "(F) Retinal  angiogenesis  assessed in P7 NtrasCA/CA and Ntras∆CA/∆CA pups  by immunostaining  of isolectin B4. Vascularized areas were normalized to total retinal  area (n = 4-8 mice  per group). Representative micrographs  are shown. Scale bars are 200 µm. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(G) FTSC  and TMR-dextran in vivo permeability  assays, comparing homogenates of hearts from NtrasCA/CA and Ntras∆CA/∆CA mice. Data normalized to organ and body weight (n = 11-18 mice  per group). Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(H) Quantification of CD45+ cell (red) infiltration into cardiac  tissue from NtrasCA/CA and Ntras∆CA/∆CA mice  normalized to DAPI.  Isolectin B4 (green) was used to label endothelial cells  (n = 4-5 mice  per group). Representative micrographs  are shown. Scale bars are 50 µm. Insets and arrows indicate sites of CD45+ cell infiltration. Data information: data are represented as mean ± S.E.M. n.s.: non-significant, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. two-tailed unpaired t-test,"
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "(I) Percentage of control and Ntras-silenced mice  showing cardiac pathologies  (n = 15-17 mice  per group). Prevalence is indicated above the bars. H&amp;E micrographs  of murine heart  sections from control and Ntras-silenced mice  are shown. Insets and arrows indicate sites of lymphocytic  infiltration. Scale bars are 100 µm. Data information: (I) Chi-square test."
NCBI gene IDs:
"Ntras: 320840"
Figure legend: "Plasma corticosterone levels at baseline, 30 minutes after induction of stress by placing mice in a clean cage, and after 90 minutes upon return in the home cage. DEX mice received an injection of dexamethasone at 0.1 mg/kg body weight 150 minutes before collecting the first blood sample (n = 5-6 animals per group). Flox and GRBATKO mice were injected with 0.02 mg/kg dexamethasone and the procedure described in (A) was followed to induce stress in order to detect potential differences between Flox and GRBATKO mice which could have been obscured by the higher concentration used in (A) (n = 6-7 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "GR mRNA levels in BAT of Flox and GRBATKO mice. Organs were harvested in the stressed condition after placing mice for 30 minutes in a clean cage. Half of the animals received a dexamethasone injection 180 minutes before onset of the procedure, controls received an injection with saline (n = 5-6 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Serum corticosterone levels under stressed conditions in Flox and GRBATKO mice (n = 5-6 animals per group). Blood glucose levels (n = 5-6 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Knockdown of GR in BAT assessed by immunoblotting. Β-Tubulin was used as loading control. Quantification of immunoblot in (A) using Image Lab (n = 6 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Representative Hematoxilin and eosin staining image of BAT of cold-exposed Flox and GRBATKO mice. Scale bar 50 µm."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Triglyceride content in BAT from Flox and GRBATKO animals (n = 5-6 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Heatmap of mean gene expression fold changes in thermogenic marker genes based on BAT microarray analysis comparing GRBATKO vs Flox control animals, indicating unchanged thermogenic marker gene expression. Color gradient ranges from 2-fold higher expression in GRBATKO over Flox controls (red) to 0.1-fold (lower) expression (blue) (n = 4 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Serum NEFA and triglycerides concentration in Flox and GRBATKO mice after fasting and refeeding (n = 6 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Total oxygen consumption plotted against body weight indicating that lower energy expenditure in GRBATKO animals is independent of body weight (n = 6-8 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Body weight in Flox and GRBATKO after 24 weeks of high-fat feeding (n = 10-13 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "BAT and iWAT weight in Flox and GRBATKO mice fed HFD for 24 weeks (n = 10-13 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "GR levels in BAT after 24 weeks high-fat feeding (n=10-13 mice per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Representative H&amp;E stained sections of BAT from Flox and GRBATKO animals. Scale bar 100 µm."
NCBI gene IDs:
"GR: 14815"
Figure legend: "qRT-PCR analysis of thermogenic marker gene expression in BAT of Flox and GRBATKO mice after high-fat feeding (n = 9-13 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "Blood glucose in Flox and GRBATKO animals after 24 weeks HFD (n = 10-13 animals per group)."
NCBI gene IDs:
"GR: 14815"
Figure legend: "A. Spinning disk time-lapse microscopy of WT and UBAP2L KO HeLa cells synchronized with double thymidine block and release (DTBR) in mitosis. The selected frames of the movies are depicted and the corresponding time is indicated in minutes. SiR-DNA was used for DNA staining."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "I, J. The time of mitotic entry (I) and from prophase to anaphase (J) was quantified. At least 50 cells per condition were analyzed for each experiment (n=3). Red bars represent the mean. K-M. The percentages of cells with abnormal mitosis (chromosome misalignments and/or DNA bridges) (K), polylobed or multinucleated daughter cells (L) and mitotic cell death (M) were quantified. At least 50 cells per condition were analyzed. N. The nuclear size of at least 1000 control and UBAP2L-downregulated interphasic cells was measured. Red bars represent the mean."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "A-C. Immunofluorescence (IF) representative pictures of WT or UBAP2L KO cells synchronized in G1/S with DTB or in early mitosis with DTBR (A) and quantification of the percentage of cells with normal (2) or abnormal number of centrosomes in interphase (B) or in early mitosis (C). At least 50 cells per condition were analyzed for each experiment. Graphs represent the mean of three biological replicates ± SD (two sample two-tailed t-test ns=non-significant)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "D-F. IF representative pictures of HeLa cells transfected with siCTL or siUBAP2L for 48h and synchronized in G1/S with DTB or in early mitosis with DTBR (D) and quantification of the percentage of cells with normal (2) or abnormal number of centrosomes in interphase (E) or in early mitosis (F). At least 50 cells per condition were analyzed for each experiment. Graphs represent the mean of three biological replicates ± SD (two sample two-tailed t-test ns=non-significant)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "G, H. IF representative pictures of WT or UBAP2L KO cells synchronized in mitosis with DTBR (G) and quantification of the percentage of cells with bipolar or non-bipolar mitotic spindle (H). At least 50 cells per condition were analyzed for each experiment. The graph represents the mean of three biological replicates ± SD (two sample two-tailed t-test ns=non-significant)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "D. WB analysis of WT or UBAP2L KO HeLa cell lysates from G1/S synchronized cells using DTB. Proteins MW is indicated in kDa. WB is representative of three independent replicates."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "E, F. IF representative pictures of G1/S synchronized WT or UBAP2L KO HeLa cells using DTB and quantification of PLK1 nuclear intensity (A.U.) ROIs are shown in the corresponding numbered panels. Scale bar, 5µm. At least 250 cells were quantified per condition for each replicate. Each dot of graphs (F) represents PLK1 nuclear intensity in a single nucleus where PLK1 signal was detectable. Interphasic cells which do not express PLK1 were excluded from the quantification. The measurements of four biological replicates are combined, red bars represent the mean (Kruskal-Wallis test with Dunn's correction **P&lt;0,01, ****P&lt;0,0001)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "B. WB analysis of control or UBAP2L-silenced HeLa lysates from mitotic cells synchronized with 1µM paclitaxel and treated with 100µg/mL CHX for the indicated times. Proteins MW is indicated in kDa. WB is representative of three independent replicates."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "C. WB analysis of WT or UBAP2L KO HeLa lysates from G1/S synchronized (DTB) cells treated with 25µM of the proteasomal inhibitor MG132 for the indicated times. Proteins MW is indicated in kDa. WB is representative of three independent replicates."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "D. Representative IF pictures of WT or UBAP2L KO HeLa cells synchronized in G1/S using DTB, in S using 2mM hydroxyurea (HU) for 20h or in G2 using 10µM of CDK1 inhibitor RO 3306 for 20h."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "IF analysis of G1/S synchronized WT or UBAP2L KO HeLa cells using DTB and transfected with the indicated flag-tagged UBAP2L protein fragments for 48h (A) Scale bar, 5µm. At least 100 cells per condition were quantified for each experiment. Graphs represent the mean of three replicates ± SD (one-way ANOVA with Sidak's correction *P&lt;0,05, **P&lt;0,01, ns=non-significant)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "IF representative pictures of WT or UBAP2L KO HeLa cells transfected with the indicated flag-tagged UBAP2L protein fragments for 48h and synchronized in mitosis using monastrol release (MR) (C) Scale bar, 5µm. At least 50 cells per condition were quantified for each experiment."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "Colony formation assay of WT or UBAP2L KO HeLa cells transiently transfected with the indicated flag-tagged UBAP2L protein fragments after 7 days of culture. Graphs represent the mean of three replicates ± SD (one-way ANOVA with Sidak's correction *P&lt;0,05, ****P&lt;0,0001, ns=non-significant)."
NCBI gene IDs:
"flag: 
UBAP2L: 9898"
Figure legend: "Representative IF images of WT or UBAP2L KO HeLa cells synchronized in G1/S using DTB and transfected with the indicated flag-tagged UBAP2L constructs and control or G3BP1/2 siRNAs for 48h (A)"
NCBI gene IDs:
"flag: 
G3BP1: 10146
UBAP2L: 9898"
Figure legend: "Representative IF images of WT or UBAP2L KO HeLa cells synchronized in G1/S using DTB and treated with vehicle (DMSO) or 25µM of MG132 for 4h (C)"
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "Representative IF images of control or UBAP2L-downregulated cells synchronized in prometaphase using 1mM monastrol and released at the indicated time points."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "E. Spinning disk time-lapse microscopy of PLK1-eGFP Knock-In (KI) HeLa cells synchronized with DTBR in mitosis. The selected frames of the movies are depicted and the corresponding time is indicated in minutes. SiR-DNA was used for DNA staining."
NCBI gene IDs:
"eGFP: 
PLK1: 5347"
Figure legend: "A, B. Representative IF pictures of WT or UBAP2L KO HeLa cells synchronized in mitosis with DTBR (A) and quantification of the relative PLK1 intensity at the inner centromere (A.U.) (B). ROIs are shown in the corresponding numbered panels. Scale bar, 5µm. At least 50 cells were quantified per condition for each experiment. Each dot represents PLK1/INCENP intensity ratio at a single centromere. The measurements of three biological replicates are combined, red bars represent the mean (Mann-Whitney test ****P&lt;0,0001)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "C, D. Representative IF pictures of WT or UBAP2L KO HeLa cells synchronized in mitosis with DTBR (C) and quantification of the relative PLK1 intensity at the centrosomes (A.U.) (D). ROIs are shown in the corresponding numbered panels. Scale bar, 5µm. At least 50 cells were quantified per condition for each experiment. Each dot represents PLK1/Pericentrin intensity ratio at a single centrosome. The measurements of three biological replicates are combined, red bars represent the mean (Mann-Whitney test ****P&lt;0,0001)."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "E, F. Representative IF pictures of WT or UBAP2L KO HeLa cells synchronized in mitosis with DTBR (E) and quantification of the relative PLK1 intensity at the midbody (A.U.) (F). ROIs are shown in the corresponding numbered panels. Scale bar, 4µm. At least 50 cells were quantified per condition for each experiment. Each dot represents PLK1/PRC1 intensity ratio at the midbody. The measurements of three biological replicates are combined, red bars represent the mean (Mann-Whitney test **P&lt;0,01)."
NCBI gene IDs:
"UBAP2L: 9898"