EMBO/SourceData huggingface dataset:
Figure legend: "(L and M) HeLa cells transfected with GFP-PI5P4K2A, 2B, 2C, and RFP-LC3 for 30 hr were loaded with 10 μM PI(3)P for 1 hr in complete medium, and then fixed and imaged on confocal microscope. Bar, 10 μm. Quantification of cells (percentage of total) showing more than 10 RFP-LC3 vesicles in the different conditions from (J) is shown in (M); n = 200 cells (mean ± SEM). See also Figure S5."
NCBI gene IDs:
"PI5P4K2A: 5305"
Figure legend: "(A) HeLa cells transfected with GFP-WIPI2B or GFP-DFCP1 preloaded with indicated concentrations of PI(5)P for 1 hr, starved in HBSS for 1 hr, and then incubated with Wm in HBSS were tracked by time-lapse microscopy for 10 min after the addition of Wm. Quantification of WIPI2B or DFCP1vesicles (percentage of those at the starting time [T0]) during the treatments are shown in the graphs."
NCBI gene IDs:
"WIPI2B: 26100
DFCP1: 53349"
Figure legend: "(B-D) HeLa cells treated with control, PI5P4K2A, 2B, and 2C siRNA were transfected with GFP-WIPI2B (B), GFP-WIPI2D (C), or GFP-DFCP1 (D); starved in HBSS (1 hr); and then incubated with Wm in HBSS. WIPI2 or DFCP1 structures were tracked and quantified as in (A)."
NCBI gene IDs:
"PI5P4K2A: 5305
WIPI2B: 26100
DFCP1: 53349"
Figure legend: "(F-H) Cell extracts from HeLa cells stably expressing GFP-WIPI2B were incubated for 3 hr with PI(5)P-containing liposomes (F and H) or PI(3)P-containing liposomes (G and H) before a pull-down experiment using the indicated beads. PS-containing liposomes were used as internal controls for both competition assays in (F and G)."
NCBI gene IDs:
"WIPI2B: 26100"
Figure legend: "(I) Lysates from HeLa cells stably expressing GFP-WIPI2B WT and GFP-WIPI2B FTTG mutant were incubated with agarose beads coated with PI(5)P and PI(3)P, eluted with SDS-PAGE sample buffer, and recovered proteins were assessed by western blotting using antibodies against GFP. See also Figure S6."
NCBI gene IDs:
"WIPI2B: 26100"
Figure legend: "(D) HeLa cells transfected with GFP-PHD3X and Strawberry-ATG16L1 for 16 hr were left in complete media (basal) or glucose-free media (glucose free) for 4 hr in the presence or absence of 100 nM YM-201636, then fixed and imaged on a confocal microscope."
NCBI gene IDs:
"ATG16L1: 55054
PHD3X: 3622"
Figure legend: "(E and F) HeLa cells transfected with GFP-PI5P4K2A, 2B, 2C, or PI5P4K2C catalytic dead and RFP-LC3 for 30 hr (E) were starved for glucose for 4 hr, then fixed and imaged on a confocal microscope. Bar, 10 μm. (F) Quantification of cells (percentage of total) showing more than ten autophagic vesicles (RFP-LC3 vesicles) in the different conditions from (F) is shown in the graph; n = 200 cells (mean ± SEM)."
NCBI gene IDs:
"LC3: 84557
PI5P4K2A: 5305
PI5P4K2C: 79837"
Figure legend: "(G) HeLa cells treated with CTR or MTMR3 siRNA for 5 days were transfected for the last 16 hr with GFP-PHD3X, Strb-ATG16L1, and myc-MTMR3 WT. Cells were starved for glucose for 4 hr, then fixed, stained with anti-myc antibodies, and imaged on a confocal microscope. Bar, 10 μm."
NCBI gene IDs:
"ATG16L1: 55054
PHD3X: 3622
MTMR3: 8897"
Figure legend: "(H) HeLa cells transfected for 16 hr with GFP-PHD3X and Strb-ATG16L1 were starved for 4 hr in HBSS or glucose-free media, then fixed, stained for PI(3)P antibodies, and imaged on a confocal microscope. Bar, 10 μm. See also Figure S7."
NCBI gene IDs:
"ATG16L1: 55054
PHD3X: 3622"
Figure legend: "(A and B) Tau increased β-catenin acetylation (Ace-β-cat) measured by immunoprecipitation using anti-β-cat, or Western blotting using anti-β-cat, anti-acetylated lysine (Ace-lys) and HT7 (probes specifically human total Tau). HEK293 cells transiently transfected with wildtype Tau40 (Tau) or the empty vector (Vec) (n = 3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Vec Data were analyzed by Student's t test"
NCBI gene IDs:
"Tau: 4137
Tau40: 4137"
Figure legend: "(C and D) β-cat mutation at K49 (K49R) but not K19 (K19R) abolished Tau-induced acetylation. HEK293 cells were co-transfected with eGFP-β-cat (WT, or K19R, or K49R, or K19R/K49R) and Tau or the empty vector for 48 h, and then immunoprecipitated using anti-GFP and Western blotting using anti-β-cat, anti-Ace-lys and HT7 (n = 3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs β-cat WT (D) ; #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs β-cat WT plus Tau (D) ; &amp;&amp;, P&lt;0.01, &amp;&amp;&amp;, P&lt;0.001 vs β-cat K19R plus Tau (D); the absence of asterisk indicates that the difference is not significant. Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"eGFP: 
β-cat: 1499
Tau: 4137"
Figure legend: "(E and F) The increased β-cat (K49) acetylation was detected in the hippocampal extracts of human Tau transgenic mice (Tau+) compared with the endogenous Tau knockout mice (Tau-), measured by Western blotting using acetylation site-specific antibody (Ace-β-cat-K49); arrowhead in panel E denoted nonspecific band identified by molecular weight. The remarkably increased levels of phosphorylated Tau at Ser199 (pS199) and AT8 epitopes were detected in Tau+ mice (at least 3 mice per group were used). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs vs Tau- (F) Data were analyzed by Student's t test"
NCBI gene IDs:
"Tau: 4137
Tau: 17762"
Figure legend: "(G and H) K18 deletion of Tau (Tau-k18(-)) attenuated Tau-induced β-cat acetylation. HEK293 cells were transfected with empty Vec, or Tau40 or Tau-k18(-) for 48 h, and the ace-β-cat was measured by immunoprecipitation using anti-β-cat and Western blotting using anti-ace-lys, anti-β-cat, and Tau5 (detecting total Tau; n = 3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Tau (H) Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"Tau: 4137
Tau40: 4137"
Figure legend: "(M and N) Expressing pseudo-phosphorylated Tau (TauS199E) further increased the K49-acetylation of β-catenin. N2a cells were transiently transfected with Vec, Tau40 or TauS199E for 48 h, levels of K49-acetylated β-cat, total β-cat and total Tau were detected by Western blotting (n=3 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Vec ; #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs β-cat WT plus vs Tau (N) Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"Tau: 4137
Tau40: 4137"
Figure legend: "(O and P) Inhibiting acetyltransferases CBP/P300 by TPOP146 (134 nM) or PCAF by L-45 (126 nM) for 24 h did not significantly decrease the K49-acetylated β-cat level in Tau-overexpressing N2a cells (n=3 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Vec Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"Tau: 4137"
Figure legend: "(D and E) Mutation of β-cat K49 abolishes its acetylation by Tau. Both myc-Tau40 and eGFP-β-cat were purified from HEK293 cells (using anti-GFP or anti-Myc beads) (D), or both from E. coli (E) (n = 3 from three independent experiments)."
NCBI gene IDs:
"eGFP: "
Figure legend: "(A and B) Expressing Tau inhibited β-cat ubiquitination in HEK293 cells, measured by immunoprecipitation using antibody against β-cat and Western blotting using anti- ubiquitination (anti-Ub) and anti-β-cat, respectively (n = 3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Vec (B) Data were analyzed by Student's t test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "(C and D) Overexpressing Tau inhibited β-cat ubiquitination in hippocampi of Tau transgenic mice, measured by immunoprecipitation using anti-Ub and Western blotting using anti-β-cat, respectively (n = 3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Tau- (D) Data were analyzed by Student's t test."
NCBI gene IDs:
"Tau: 4137"
Figure legend: "(E and F) Expressing Tau-k18(-) attenuated Tau-induced β-cat elevation. HEK293 cells were transfected with Vec, or Tau-40, or Tau-k18(-) for 24 h, and then were treated with Chx for 12 h or 24 h, followed by Western blotting. β-cat protein level was normalized to β-actin (n=4 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Tau (F) Data were analyzed by Two-way repeated-measures ANOVA"
NCBI gene IDs:
"Tau: 4137"
Figure legend: "(G and H) K49 acetylation of β-cat inhibited its ubiquitination. HEK293 cells were co-transfected with HA-ubiquitin and GFP-β-cat or pseudo-acetylated K49-β-catenin (K49Q), and then β-cat was immunoprecipitated by anti-GFP and blotted by anti-Ub and anti-β-cat (n=3 from three independent experiments). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs β-cat WT Data were analyzed by Student's t test."
NCBI gene IDs:
"GFP: 
HA: 
β-cat: 1499
β-catenin: 1499"
Figure legend: "(I and J) K49 acetylation of β-cat inhibited its degradation. HEK293 cells were transfected with β-cat WT or K49Q for 24 h, and then treated with cycloheximide (Chx, 100 μg/ml) for 12 h or 24 h (n=4 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs β-cat WT Data were analyzed by Two-way repeated-measures ANOVA"
NCBI gene IDs:
"β-cat: 1499"
Figure legend: "β-cat mutation at K49 (K49R) restored the degradation of β-catenin. HEK293 cells were transfected with β-cat WT or co-transfected with β-cat WT and Tau40, or co-transfected with β-cat K49R and Tau40 for 24 h, and then treated with cycloheximide (Chx, 100 μg/ml) for 12 h or 24 h (n=4 biological replicates each group)."
NCBI gene IDs:
"β-cat: 1499
Tau40: 4137"
Figure legend: "β-cat mutation at K49 (K49R) restored the degradation of β-catenin. HEK293 cells were transfected with β-cat WT or co-transfected with β-cat WT and Tau40, or co-transfected with β-cat K49R and Tau40 for 24 h, and then treated with cycloheximide (Chx, 100 μg/ml) for 12 h or 24 h (n=4 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs Tau+β-cat WT (L) Data were analyzed by Two-way repeated-measures ANOVA"
NCBI gene IDs:
"β-cat: 1499
Tau: 4137
Tau40: 4137"
Figure legend: "(M and N) K49 acetylation of β-cat inhibited its phosphorylation at N-terminal domain. HEK293 cells were transfected with GFP-vec, or eGFP-β-cat (WT) or K49Q for 48 h, then treated with wortmannin (WO, 1 μM) plus GF109203X (GFX, 1 μM) to activate GSK-3β for 1 h followed by Western blotting (n=3 biological replicates each group). Data information: Data are presented as mean ± SEM; *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001 vs β-cat WT plus WO/GFX (N). data in (N) was analyzed by one-way ANOVA."
NCBI gene IDs:
"eGFP: 
GFP: 
β-cat: 1499"
Figure legend: "(A and B) Expressing Tau-k18(-) abolished its effect on β-cat K49-acetylation and c-cas-3 (cleaved caspase-3). N2a cells were transfected with Vec, or Tau40, or Tau-k18(-) for 48 h, and then treated with 0.5 μM staurosporine (STP, an apoptotic inducer) for 4 h, followed by Western blotting (n=3 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs vec (B) #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs Vec plus STP (B) ; $$$, P&lt;0.001 vs Tau plus STP (B) Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"Tau: 4137
Tau40: 4137"
Figure legend: "Expressing Tau-k18(-) or Tau-2CA abolished its anti-apoptotic function. ; N2a cells, transfected with myc-Vec, or myc-Tau40, or myc-Tau-k18(-), or myc-Tau-2CA for 48 h, were then treated with 0.5 μM STP for 4 h followed by flow cytometry using Annexin-FITC (C) Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Tau plus STP Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"myc: 
Tau: 4137
Tau40: 4137"
Figure legend: "Expressing Tau-k18(-) or Tau-2CA abolished its anti-apoptotic function. N2a cells, transfected with eGFP-Vec, or eGFP-Tau40, or eGFP-Tau-k18(-), or Tau-2CA for 48 h, were then treated with 0.5 μM STP for 4 h followed by co-staining with c-cas-3 (red) (D In panel D (middle row), the Tau expression (white arrowheads) was largely not co-localized with c-casp-3 (yellow arrowheads) in the same visual field (n=4~8 biological replicates each group; scale bar, 10 μm)."
NCBI gene IDs:
"eGFP: 
Tau: 4137
Tau40: 4137"
Figure legend: "Expressing Tau-k18(-) or Tau-2CA abolished its anti-apoptotic function. N2a cells, transfected with eGFP-Vec, or eGFP-Tau40, or eGFP-Tau-k18(-), or Tau-2CA for 48 h, were then treated with 0.5 μM STP for 4 h followed by co-staining with c-cas-3 (red) The apoptotic rate (E) was presented as the ratio of yellow (c-casp-3 and GFP co-stained) to green (the total GFP-expressing cells) staining. Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Tau plus STP Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"eGFP: 
Tau: 4137
Tau40: 4137"
Figure legend: "Expressing Tau-k18(-) or Tau-2CA abolished its anti-apoptotic function. N2a cells, transfected with myc-Vec, or myc-Tau40, or myc-Tau-k18(-), or myc-Tau-2CA for 48 h, were then treated with 0.5 μM STP for 4 h followed by CCK8 assay (F). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Tau plus STP ♧♧♧, P&lt;0.001 vs vec Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"myc: 
Tau: 4137
Tau40: 4137"
Figure legend: "(G) Non-acetylation modifiable mutation of β-cat at K49 (K49R), not K19 (K19R), abolished the anti-apoptotic effect of Tau. N2a cells were co-transfected with Tau40 and β-cat WT, or K49R, or K19R for 48 h, and then treated with 0.5 μM STP for 4 h. The cell viability was measured by CCK8 assay (n=8 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Vec plus STP ; #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs vs β-cat + Vec plus STP (G) ; $$$, P&lt;0.001 vs β-cat + Tau plus STP (G) ; &amp;&amp;&amp;, P&lt;0.001 vs β-cat K49R+ Tau plus STP (G). ♧♧♧, P&lt;0.001 vs vec Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"β-cat: 12387
Tau: 4137
Tau40: 4137"
Figure legend: "(H) Expressing β-cat K49Q most significantly increased cell viability. N2a cells were transfected with β-cat WT or K49R or K49Q or the empty Vec for 48 h, and then treated with 0.5 μM STP for 4 h. The cell viability was measured by CCK8 assay (n=8 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Vec plus STP ; #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs β-cat plus STP (H), ; $$$, P&lt;0.001 vs β-cat K49Q plus STP (H) ♧♧♧, P&lt;0.001 vs vec Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"β-cat: 12387"
Figure legend: "(I) Overexpressing Ser199-phosphorylation mimic Tau (TauS199E) further augmented cell viability. N2a cells were transfected with empty vec, Tau40 or TauS199E for 48h and then treated with 0.5 μM STP for 4 h. The cell viability was measured by CCK8 assay (n=8 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Vec plus STP ; #, P&lt;0.05, ##, P&lt;0.01, ###, P&lt;0.001 vs vs Tau plus STP (I) Data were analyzed by one-way ANOVA."
NCBI gene IDs:
"Tau: 4137
Tau40: 4137"
Figure legend: "Expressing β-cat K49Q increased its nuclear translocation. HEK293 cells were transfected with eGFP-β-cat WT or K49Q for 48 h, and then β-cat protein level in different fractions was measured by Western blotting"
NCBI gene IDs:
"eGFP: 
β-cat: 1499"
Figure legend: "Expressing β-cat K49Q increased its nuclear translocation. HEK293 cells were transfected with eGFP-β-cat WT or K49Q for 48 h, and then β-cat protein level in different fractions was measured by Western blotting Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs β-cat WT (C) Data were analyzed by Student's t test"
NCBI gene IDs:
"eGFP: 
β-cat: 1499"
Figure legend: "Expressing β-cat K49Q increased its nuclear translocation. HEK293 cells were transfected with eGFP-β-cat WT or K49Q for 48 h and immunofluorescent labeling using a laser scanning confocal microscope (D). The nuclei (blue) was stained by DAPI (n=3 from three independent experiments, scale bar, 2 μm)."
NCBI gene IDs:
"eGFP: 
β-cat: 1499"
Figure legend: "(E) Expressing β-cat K49Q further increased its transcription activity measured by dual Topflash/Fopflash reporter luciferase assay after transient transfection of β-cat WT, or K49Q, or Vec in HEK293 cells for 48 h, and the luciferase activity was normalized by β-cat WT or K49Q protein level (n=7 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Vec ; ##, P&lt;0.01, ###, P&lt;0.001 vs β-cat WT Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"β-cat: 1499"
Figure legend: "(F) Expressing Tau-k18(-) abolished Tau-induced upregulation of mRNA levels of survival genes (bcl2 and survivin) and dkk1 (downstream of Wnt signaling) in N2a cells measured by Q-PCR (n=6~9 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Tau (F Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"bcl2: 12043
survivin: 11799
dkk1: 13380
Tau: 4137"
Figure legend: "(G) Expressing Tau-k18(-) or Tau-2CA abolished Tau-induced Wnt/β-catenin transcriptional activation measured by dual reporter luciferase activity assay in HEK293 cells (n=7 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs vs Tau Data were analyzed by one-way ANOVA"
NCBI gene IDs:
"Tau: 4137"
Figure legend: "(H) Expressing phosphor-mimic TauS199E (TauS199E) further increased the transcription activity of Wnt/TCF/LEF1 by Tau in HEK293 cells (n=7 biological replicates each group). Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Vec ; ##, P&lt;0.01, ###, P&lt;0.001 vs Tau (H). Data were analyzed by , one-way ANOVA"
NCBI gene IDs:
"Tau: 4137"
Figure legend: "The increased mRNA levels of bcl2, survivin and dkk1 in the AD brains compared with the age-/sex-matched controls detected by Q-PCR (n=7 each group) Data information: Data are expressed as mean ± SEM, *, P&lt;0.05; **, P&lt;0.01, ***, P&lt;0.001 vs Ctrl (I) Data were analyzed by Student's t test"
NCBI gene IDs:
"bcl2: 596
survivin: 332
dkk1: 22943"
Figure legend: "The increased mRNA levels of bcl2, survivin and dkk1 in the AD brains compared with the age-/sex-matched controls detected by Q-PCR (n=7 each group), and the positive correlations with the increased K49-acetylated β-catenin (see Fig. 1I, J) analyzed by Pearson analysis."
NCBI gene IDs:
"bcl2: 596
survivin: 332
dkk1: 22943"
Figure legend: "D, Western blotting of HSP70, tsg 101, CD81, CD63, and CD9 in EVs, whole blood, and blood clot derived from suckling mice infected with DMEM or NoVΔB2. Staining of β-actin was used as a loading control."
NCBI gene IDs:
"B2: 962118"
Figure legend: "Immunoblot of lysates prepared from the indicated SAMHD1-proficient (+/+), deficient (-/-) and rescue (WT, H233A) cell line pairs with the indicated antibodies. Representative of 2 independent experiments."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Proliferation inhibition analysis of ara-C and RNRi combination treatment in SAMHD1+/+ or -/- THP-1 cells. Error bars indicate s.e.m. of two (HU and dF-dC) or three (3-AP) independent experiments, each performed in duplicate."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Ara-C EC50 values plotted as a function of RNRi concentration in SAMHD1+/+, -/- and rescue (WT, H233A) THP-1 cell line pairs. EC50 values in the absence of RNRi are indicated by the black and red dotted line. Error bars indicate s.e.m. of two (HU and dF-dC) or three (3-AP) independent experiments, each performed in duplicate."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Drug synergy plots for ara-C and the indicated RNRi in SAMHD1+/+, -/- and rescue (WT, H233A) cell line pairs. Each data point indicates an average delta score from a single dose-response matrix experiment performed in duplicate. Zero, &gt;0, or &lt;0 corresponds to additive, synergy, or antagonism, respectively, whilst &gt;5 indicates strong synergy. The horizontal line and the error bars indicate the mean and s.d., respectively, statistical significance was determined using a two-tailed unpaired t-test: ns, not significant, P ≥ 0.05; *, P &lt; 0.05; **, P &lt; 0.01; ***, P &lt; 0.001; ****, P &lt; 0.0001."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Immunoblot analysis of lysates prepared from SAMHD1+/+ or -/- THP-1 cells treated for 24 h with ara-C and HU, as indicated. Representative of 3 independent experiments."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Kaplan-Meier analysis of NOD/SCID mice injected i.v. with luciferase-expressing SAMHD1+/+ or -/- THP-1 cell clones (day 0) and treated with ara-C and/or HU as indicated (day 6). n = 6 per treatment group."
NCBI gene IDs:
"luciferase: 
SAMHD1: 25939"
Figure legend: "Kaplan-Meier analysis of NOD/SCID mice injected i.v. with luciferase-expressing SAMHD1+/+ or -/- HL-60/iva cell clones (day 0) and treated with ara-C and/or HU as indicated (day 6). n = 6 per treatment group."
NCBI gene IDs:
"luciferase: 
SAMHD1: 25939"
Figure legend: "Kaplan-Meier analysis of NOD/SCID mice injected i.v. with luciferase-expressing SAMHD1+/+ THP-1 cell clone (day 0) and treated with ara-C and/or dF-dC as indicated (day 6). n = 7 per treatment group."
NCBI gene IDs:
"luciferase: 
SAMHD1: 25939"
Figure legend: "Kaplan-Meier analysis of CD45.2 C57BL/6J mice injected i.v. with murine MLL-AF9-transformed AML blasts (day 0) and treated with ara-C and/or HU days 20-24. n = 5 per treatment group, except for vehicle (n = 4)."
NCBI gene IDs:
"MLL-AF9: 214162"
Figure legend: "Intracellular dNTP measurements using a primer-extension assay in SAMHD1+/+ THP-1 cells treated for 4 or 24 h with either 50 µM HU (middle panel) or 2.5 nM 3-AP (right panel), ratios of dCTP-to-dATP were calculated. Bars indicate mean values of three independent experiments, error bars indicate s.e.m. Statistical analyses were done using unpaired two-tailed t-tests: **, P &lt; 0.01."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Intracellular ara-CTP (upper panel) and dCTP:dATP ratio (lower panel) in the indicated cell lines following the indicated treatment determined using HPLC-MS/MS. SAMHD1-/- THP-1 cells were treated with 500 nM ara-C and SAMHD1+/+ THP-1 cells were treated with either solvent, 500 nM ara-C or a combination of 500 nM ara-C and an RNRi (HU, 50 µM; dF-dC, 10 nM; 3-AP, 150 nM) for 24 h prior. Values relative to mean ara-CTP amounts in ara-C treated SAMHD1+/+ THP-1 cells shown (indicated by dashed line). Circles, columns and error bars correspond to individual values, means and s.e.m. of at least three experiments performed independently."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "Quantificaiton of dCK phosphorylation at serine-74 (S74) with respect to total dCK in SAMHD1+/+ THP-1 cells treated with either solvent, 500 nM ara-C or a combination of 500 nM ara-C and an RNRi (HU, 50 µM; dF-dC, 10 nM; 3-AP, 150 nM) for 24 h. Circles and squares, columns and error bars correspond to individual measurements, means and s.e.m. of one representative out of two independent experiments performed in triplicates."
NCBI gene IDs:
"SAMHD1: 25939"
Figure legend: "(A, B) Knockdown of hnRNPA3 increases poly-GA expression while expressions of EGFP protein levels are not altered upon knockdown of hnRNPs. The control (\"x0\") vector lacks the G4C2 repeats but still contains the 5' flanking region and 3x TAG. ANOVA, Dunnett."
NCBI gene IDs:
"hnRNPA3: 220988"