EMBO/SourceData huggingface dataset:
Figure legend: "A-D Adult wing phenotype of knock-down of Gapdh (C765&gt;GapdhRNAi), over-expression of full-length Ecd (C765&gt;ecd), over-expression of C-terminally truncated form of Ecd (C765&gt;ecdDN), and knock-down of ecd (C765&gt;ecdRNAi) in the whole wing disc (see Appendix Fig S3A for the expression pattern of C765-Gal4). Images of adult wings of control (A), C765&gt;GapdhRNAi (A'), C765&gt;ecd (B), C765&gt;ecdDN (C), and C765&gt;ecdRNAi (D) male flies. (A'') Overlay of the wings shown in A and A'. (C') Overlay of control wing and wing shown in C and C'. Loss of Ecd throughout the wing discs produces adults with vestigal wings (D). In contrast, the effects of ecdDN over-expression or Gapdh knock-down are less severe on tissue survival, giving rise to smaller wings with altered proportions. Images consist of a series of tiles that were stitched together by the microscope software. Scale bar= 500 μm. E, F Tukey box-and-whiskers plot showing quantifications of the area (E) and shape (aspect ratio: major/minor axis, F) of control, C765&gt;GapdhRNAi, C765&gt;ecd, and C765&gt;ecdDN wings. Lower and upper hinges correspond to the first and third quartiles, vertical lines extend to ± 1.5 times the interquartile range. Sample size is indicated for each perturbation. Loss of Gapdh or over-expression of EcdDN leads to smaller wings that are increased along the anterior-posterior (A/P) axis compared to the proximal-distal (P/D) axis. Statistical analysis was performed using one-way ANOVA, followed by Dunnett's multiple comparisons test. ***p≤0.001. "
NCBI gene IDs:
"Gal4: 
ecd: 38291
Ecd: 38291
Gapdh: 35728///32545
Gapdh: 32545///35728"
Figure legend: " A-B' Time-controlled knock-down of ecd in the dorsal compartment of the wing discs (see Fig 1C for the expression pattern of apGalts). IF of control (A, A') and apGalts&gt;ecdRNAi (B, B') wing discs, stained for the Hedgehog (Hh) pathway components Smoothened (Smo, A, B), and Ci155 (A', B'). Next to the images are quantifications of the respective staining in the dorsal versus ventral compartment of control (n=5) and apGalts&gt;ecdRNAi (n=5) wing discs. Graphs show mean (thick line) ± SD (thin lines). Dashed yellow lines indicate the position of the A/P boundary. Statistical analyses (t-test) revealed significant differences in Smo and Ci155 expression in the dorsal compartment between control and apGalts&gt;ecdRNAi (Smo: p≤0.001, Ci155: p≤0.001) wing discs. Thus, loss of Ecd elevates Smo levels on the plasma membrane and increases the range of Ci155 stabilization in the anterior compartment. Wing discs were analyzed 48 h after RNAi induction. Scale bars= 50 μm. "
NCBI gene IDs:
"apGal: 
Ecd: 38291
ecd: 38291"
Figure legend: "C-E' Time-controlled knock-down of metabolic enzymes in the dorsal compartment of the wing disc. IF of apGalts&gt;PfkRNAi, apGalts&gt;GapdhRNAi, and apGalts&gt;Glo1RNAi wing discs, stained for Smo (C, D, E) and Ci155 (C', D', E'). Next to the images are quantifications of the respective staining in the dorsal versus ventral compartment of apGalts&gt;PfkRNAi (n=5), apGalts&gt;GapdhRNAi (n=5), and apGalts&gt;Glo1RNAi (n=6) wing discs. Graphs show mean (thick line) ± SD (thin lines). Dashed yellow lines indicate the position of the A/P boundary. Statistical analyses (t-test) revealed significant differences in Smo and Ci155 expression in the dorsal compartment between control and apGalts&gt;PfkRNAi (Smo: p≤0.001, Ci155: p≤0.01), apGalts&gt;GapdhRNAi (Smo, Ci155: p≤0.05), or apGalts&gt;Glo1RNAi (Smo: p≤0.001, Ci155: p≤0.05) wing discs. Thus, loss of Pfk, Gapdh or Glo1 elevates Smo levels on the plasma membrane and increases the range of Ci155 stabilization in the anterior compartment. Wing discs were analyzed 120 h after RNAi induction. Scale bars= 50 μm."
NCBI gene IDs:
"apGal: 
Gapdh: 35728///32545
Gapdh: 32545///35728
Glo1: 35656
Pfk: 36060"
Figure legend: " A-B Time-controlled knock-down of ecd in the dorsal compartment of the wing discs (see Fig 1C for the expression pattern of apGalts). IF of control (A) and apGalts&gt;ecdRNAi (B) wing discs, stained for Hh. Next to the images are quantifications of the respective staining in the dorsal versus ventral compartment of control (n=4) and apGalts&gt;ecdRNAi (n=5) wing discs. Graphs show mean (thick line) ± SD (thin lines). Dashed yellow lines indicate the position of the A/P boundary. Statistical analysis (t-test) revealed no significant differences in Hh expression in the dorsal compartment between control and apGalts&gt;ecdRNAi wing discs. Thus, loss of Ecd does not affect Hh production and spreading. Wing discs were analyzed 48 h after RNAi induction. Scale bars= 50 μm. "
NCBI gene IDs:
"apGal: 
Ecd: 38291
ecd: 38291"
Figure legend: "C-F Time-controlled knock-down of ecd in the dorsal compartment of dispatched mutant wing discs (dispS037707, apGalts&gt;ecdRNAi, see Fig 1C for the expression pattern of apGalts). IF of control (C), apGalts&gt;ecdRNAi (D), dispS037707 (E) and dispS037707, apGalts&gt;ecdRNAi (F) wing discs expressing the decapentaplegic (dpp) reporter gene dpp-lacZ (dppZ), stained for dppZ. Next to the images are quantifications of the respective stainings (n=4 (C, F), n=5 (D), n=3 (E)). Graphs show mean (thick line) ± SD (thin lines). Dashed yellow lines indicate the position of the A/P boundary. Statistical analyses (t-test) revealed a significant difference in dppZ expression in the dorsal compartment between control and apGalts&gt;ecdRNAi (p≤0.01) (C vs D), and dispS037707 and dispS037707, apGalts&gt;ecdRNAi (p≤0.01) wing discs (E vs F). Loss of Ecd elevates the expression of dppZ in a broader than normal stripe. However, lack of Ecd function alone is not sufficient to trigger dpp expression, which instead depends on the presence of Hh. Wing discs were analyzed 48 h after RNAi induction. Scale bars= 50 μm."
NCBI gene IDs:
"apGal: 
disp: 44274
ecd: 38291
Ecd: 38291"
Figure legend: " G-J IF of dispS037707 (G, I) and dispS037707, apGalts&gt;ecdRNAi (H, J) wing discs, stained for Smo (G, H) and Ci155 (I, J). Next to the images are quantifications of the respective stainings (n=2 (G, H), n=3 (I), n=4 (J)). Graphs show mean (thick line) ± SD (thin lines). Dashed yellow lines indicate the position of the A/P boundary. Although statistical analyses (t-test) revealed no significant differences in Smo and Ci155 expression in the dorsal compartment between control and dispS037707, apGalts&gt;ecdRNAi wing discs, loss of Ecd slightly induces an increased Smo accumulation and Ci155 stabilization independently of Hh. Wing discs were analyzed 48 h after RNAi induction. Scale bars= 50 μm. "
NCBI gene IDs:
"apGal: 
disp: 44274
Ecd: 38291
ecd: 38291"
Figure legend: " A-A''' Time-controlled knock-down of ecd or Gapdh in the dorsal compartment of the wing discs (see Fig 1C for the expression pattern of apGalts). The plasma membrane potential was detected using DiBAC4(3). The fluorescence intensity of DiBAC4(3) increases upon plasma membrane depolarization. (A) The change in plasma membrane potential upon genetic perturbation was quantified as the fold change in the ratio of DiBAC4(3) signal intensity in the dorsal to ventral compartment (control (n=19), apGalts&gt;ecdRNAi (n=36) and apGalts&gt;GapdhRNAi (n=18) wing discs). Error bars indicate ± SD. t-test, *p≤0.05, ***p≤0.001. (A'-A''') Representative images of DiBAC4(3) assay in control (A'), apGalts&gt;ecdRNAi (A''), and apGalts&gt;GapdhRNAi (A''') wing discs 48 h or 120 h after RNAi induction, respectively. V/D indicates the boundary between the ventral and dorsal compartments. Next to the maximal projections are shown the sum projections of cross-sections along the A/P axis. Loss of Ecd or Gapdh alters the plasma membrane potential. Scale bars= 50 μm. B Structure of the N-acylethanolamide analogue PAC-NAE. PAC-NAE contains a C15 fatty acid featuring diazirine and alkyne (red) modifications. C-C''' Time-controlled knock-down of ecd or Gapdh in the dorsal compartment of the wing discs (see Fig 1C for the expression pattern of apGalts). PAC-NAE uptake was quantified as the ratio of PAC-NAE signal intensity in the dorsal to ventral compartments. Shown is the fold change from control in apGalts&gt;ecdRNAi and apGalts&gt;GapdhRNAi wing discs (C). For each condition, dorsal/ventral PAC-NAE signal intensity was quantified for 5 wing discs. Error bars indicate ± SD. test-test, **p≤0.01, ***p≤0.001. (C'-C''') Representative images of PAC-NAE uptake in control (C'), apGalts&gt;ecdRNAi (C'') and apGalts&gt;GapdhRNAi (C''') wing discs at 48 h or 120 h after RNAi induction, respectively. Loss of Ecd or Gapdh blocks the uptake of PAC-NAE. Dashed green lines mark the D/V boundary. Scale bars= 50 μm."
NCBI gene IDs:
"apGal: 
ecd: 38291
Ecd: 38291
Gapdh: 32545///35728"
Figure legend: "MoLC from six different donors (n=6) were left untreated (NT) or incubated with LPS for 48h and analysed for S100A9 expression by RT-qPCR (left graph). MoLC treated as before were then transduced with HIV-GFP vectors for 48h and GFP+ cells were analysed by flow cytometry and data were graphically represented as fold relative to non-treated MoLC (right graph). Black horizontal line correspond to the mean. When applicable, student t-test was performed to determine statistical significance (***P&lt;0.001)."
NCBI gene IDs:
"GFP: 
S100A9: 6280"
Figure legend: "Cells transfected as above were pre-treated or not with nevirapine and transduced with HIV-GFP vectors for 48h. The percentage of GFP+ MoLC was analysed by flow cytometry. Data represent means values ± SD from two different donors."
NCBI gene IDs:
"GFP: "
Figure legend: "Human epidermal cells treated as before were challenged for 48h with 20 and 200ng of p24 of HIV-Luc. When indicated cells were pre-treated with 10μM Nevirapine for 30 min before viral challenge. Luciferase activities measured in duplicates for each condition from two (infection with 20 ng P24) or three (infection with 200 ng P24) different skin samples were represented on a graph as means of relative luminescence units (RLU) ± SD."
NCBI gene IDs:
"Luc: "
Figure legend: "The experiment shown is representative of four donors for HIV-1-R5 (n=4) and three donors for HIV-1 primary isolates (n=3). Data collected from all donors were compiled and represented as bar charts for cells challenged with HIV-1-R5 and HIV-1 primary isolates. Nevirapine (Nev) (10μM) was used as control when indicated and data were represented as means ± SD of HIV-Gag+ cells from at least 2 (for siS100A9-MoLC infected by HIV-1 primary isolates) to 3 donors. When applicable, student t-test was performed to determine statistical significance (*P&lt;0.1;**P&lt;0.01;***P&lt;0.001)."
NCBI gene IDs:
"S100A9: 6280"
Figure legend: "E2C- and S100A9-expressing HEK293T cells were infected with the indicated MOI of HIV-GFP for 48h and GFP+ cells were analysed by flow cytometry. Mean values ± SD from four independent experiments (n=4) were normalized to the E2C-HEK293T control and represented in graph."
NCBI gene IDs:
"E2C: 
GFP: 
S100A9: 6280"
Figure legend: "E2C- and S100A9-expressing CHO cells were challenged with indicated MOI of MMLV-YFP vectors for 24h or 48h. The percentages of YFP+ CHO infected cells analysed by flow cytometry and data were represented on graphs."
NCBI gene IDs:
"E2C: 
S100A9: 6280"
Figure legend: "E2C- or S100A9-expressing THP1 were left uninfected (/) or infected for 3 h with the indicated p24 amounts of HIV-1 NL4-3/Nef-IRES-Renilla viruses containing BlaM-VPR. Cells were processed for Blam-Vpr assay and the percentage of cleaved CCF2 was analysed by flow cytometry. The mock condition corresponds to unstained E2C-expressing cells. Dot-plots from a representative experiment are shown."
NCBI gene IDs:
"E2C: 
Nef: 
BlaM: 59687626
S100A9: 6280
VPR: 155807"
Figure legend: "Schematic description of fate-of-capsid assay is displayed above. HEK293T expressing E2C or S100A9 were transduced with HIV-Luc (10ng p24) for 4 h. Cells were then subjected to the fate of capsid assay (see methods) in order to pull-down intact cores (CA cores) (recovered in the pellet). Equal volumes from the soluble (top fraction) and pelleted (down fraction) materials were analyzed by western blotting in order to evaluate the presence of p24 (CA), CypA, S100A9 and E2C proteins."
NCBI gene IDs:
"E2C: 
Luc: 
S100A9: 6280"
Figure legend: "Colorimetric HIV-1 RTase activity was performed with 10µg of immunoprecipitated and eluted E2C or S100A9 in presence of increasing MgCl2 concentrations. Means ± SD colorimetric data obtained from at least three independent experiments were normalized to E2C-containing conditions and shown on graphs."
NCBI gene IDs:
"E2C: 
S100A9: 6280"
Figure legend: "(A) The time courses of normalized FRET ratio (Mean ± SEM) of calcium biosensor before and after shockwave stimulation in HBSS Ca2+ medium with (yellow line, n = 6, N = 3) or without the Piezo1 expression (black line, n = 6, N = 3). (B) The representative FRET/ECFP ratio images of calcium biosensor in Piezo1-expressed HEK cells before (top left) and after (top right) LIS stimulation. Phase image with laser initiating point (left) and fluorescent image indicating Piezo1 expression (right) were also shown on the bottom panels (Scale bar, 40 μm). Color scale bars in the figures are to show the FRET/ECFP ratios, with cold and hot colors representing low and high ratios, respectively. (C) The time course of normalized FRET ratio (Mean ± SEM) of calcium biosensor in the Piezo1-expressing HEK cells before and after multiple shockwave stimulations in HBSS Ca2+ medium (n = 6, N = 3)."
NCBI gene IDs:
"Piezo1: 234839"
Figure legend: "(A) The representative ECFP/FRET ratio images of FAK biosensor in Piezo1 overexpressed HEK cells before (left) and after (right) shockwave stimulation (Scale bar, 40 μm). Color scale bars in the figures are to show the ECFP/FRET ratios, with cold and hot colors representing low and high ratios, respectively. (B) The time courses of normalized ECFP/FRET ratio (Mean ± SEM) of FAK FRET biosensor before and after shockwave stimulation in HBSS Ca2+ media with (green line, n = 16, N = 3) or without Piezo1 (black line, n = 10, N = 3). (C) The percentage change of FRET ratio of the FAK FRET biosensor in HEK cells with or without Piezo1 after shockwave stimulation in HBSS Ca2+ medium. *P &lt; 0.05 from student two-tailed t-test."
NCBI gene IDs:
"Piezo1: 234839"
Figure legend: "(A) The representative ECFP/FRET ratio images of FAK biosensor in Piezo1 expressing HEK cells before and after 25 μM Yoda1 stimulation (Scale bar, 10 μm). Color scale bars in the figures indicate ECFP/FRET ratios, with cold and hot colors representing low and high ratios, respectively. (B) The time courses of normalized FRET ratio (Mean ± SEM) of FAK FRET biosensor before and after Yoda1 stimulation with (pink line, n = 10, N = 3) or without Piezo1 (black line, n = 11, N = 3). The grey line represents the DMSO control group (n = 8, N = 3)."
NCBI gene IDs:
"Piezo1: 234839"
Figure legend: "(C) The representative FRET/ECFP ratio images of the calcium biosensor in the Piezo1-expressing HEK cells before and after 25 μM Yoda1 stimulation (Scale bar, 10 μm). Color scale bars in the figures indicate FRET/ECFP ratios, with cold and hot colors representing low and high ratios, respectively. (D) The time courses of normalized FRET ratio (Mean ± SEM) of calcium biosensor in the Piezo1-expressing HEK cells (purple line, n = 9, N = 3) before and after 25 μM Yoda1 stimulation in culture medium."
NCBI gene IDs:
"Piezo1: 234839"
Figure legend: "(A)&amp;(B), The time courses (Mean ± SEM) (A) and representative images (Scale bar, 10 μm) of FRET ratio of SHP2 biosensor (Y542F) before and after 25 μM or 0.5 μM Yoda1 stimulation in cells with or without Piezo1 overexpression (red bar, n = 5, N=3; blue bar, n = 5, N=3; purple bar, n = 11, N=3; green bar, n = 5, N=3)."
NCBI gene IDs:
"Piezo1: 234839"
Figure legend: "D-E) Immunofluorescence  against CITRINE  (electroporated  cells), SOX10   (Schwann cell precursors and Schwann cells) and ISL1  (sensory  and sympathetic neurons) on embryos electroporated  with either control CRISPR  plasmid (D) or a CRISPR  plasmid containing a guide-RNA against Sox8  (E). CITRINE+ cells populate the developing dorsal root ganglia (DRG) and peripheral nerves. Examples of CITRINE+/SOX10+ cells shown by arrowheads. Asterisks show ventral boundary cap glia. Scale bar is 50 µm in overviews and 10 µm in insets. F) Quantification of the fate distribution of CITRINE+ cells as a % between glial  (SOX10+) cells, sensory neurons (ISL1+) or neither (SOX10-/ISL1-) in the DRG  of control and  Sox8KD chick embryos. Biological replicates - N=3 embryos  per condition (wild type versus Sox8  KD). Data represented as mean±SEM. Statistical significance determined using the Holm-Sidak method (alpha = 0.05) (multiple t-tests, unpaired). SOX10+ cells: p=0.7901, ISL1+ cells: p=0.9206, SOX10-/ISL1- cells: p=0.9206. For statistical significance: non-significant - p-value≥0.05. G) Quantification of (left) the % of SOX10+ cells in the peripheral nerves  that are CITRINE+ in control and Sox8KD chick embryos  and (right) the % of PH3+ CITRINE+ cells corresponding to proliferative  cells. Biological replicates - N=4 embryos  per condition for SOX10+ distribution and N=2 for PH3 quantification (wild type versus Sox8  KD). Data represented as mean±SEM. Statistical significance determined unpaired t-test with two-tailed p value. SOX10+ cells: p=0.0044, PH3+ cells: p=0.4929. For statistical significance: non-significant - p-value≥0.05, ** - p-value&lt;0.01. Data information: In total, 6 embryos  were analyzed for the control electroporation  and 7 embryos  were analyzed for the Sox8  knock down electroporation  and data depicted in graphs correspond to mean±SEM per embryo  (corresponding to biological replicates): 3-4 electroporated embryos  were analyzed per condition (control and SOX8 knock down) with 4-5 sections stained  and analyzed per embryo  per region of interest (DRG, DRG: dorsal root ganglia, sg: sympathetic ganglion"
NCBI gene IDs:
"CRISPR: 
Sox8: 395483
SOX8: 395483"
Figure legend: "I) Immunofluorescence  against CITRINE  (electroporated  cells), SOX10 (Schwann cell precursors  and Schwann cells) and TH  (sympathetic neurons and chromaffin cells) of the  sympathoadrenal domain on control and  Sox8KD embryos. CITRINE+/SOX10+ cells shown by empty arrowheads while CITRINE+/TH+ cells are shown by filled arrowheads. Scale bar = 50 µm. J) Quantification of the fate distribution of CITRINE+ cells as a % between glial  (SOX10+) cells, chromaffin cells  (TH+) or neither (SOX10-/TH-) in the proximity of the  dorsal aorta and  visceral nerve of wild type and Sox8  KD chick embryos. Biological replicates - N=4 embryos  per condition. Data represented as mean±SEM. Statistical significance determined using the Holm-Sidak method (alpha = 0.05) (multiple t-tests, unpaired). SOX10+ cells: p=0.0067, TH+ cells: p=0.0067, SOX10-/TH- cells: p=0.8819. For statistical significance: non-significant - p-value≥0.05, * - p-value&lt;0.05. Data information: In total, 6 embryos  were analyzed for the control electroporation  and 7 embryos  were analyzed for the Sox8  knock down electroporation  and data depicted in graphs correspond to mean±SEM per embryo  (corresponding to biological replicates): 3-4 electroporated embryos  were analyzed per condition (control and SOX8  knock down) with 4-5 sections stained  and analyzed per embryo  per region of interest Sympathoadrenal domain)."
NCBI gene IDs:
"Sox8: 395483
SOX8: 395483"
Figure legend: "(A-C) Measurement of autophagy during nutrient starvation in HT29 cells stably expressing YFP-LC3. (A) Increased LC3 puncta per cell following β‐catenin knockdown with siRNA compared with a non‐targeting (NT) control siRNA. Columns show autophagosome numbers (mean±s.e.m., n&gt;200 cells in &gt;20 fields of view per experiment of three independent treatments) assessed under normal and starved (2 h) conditions with β‐catenin or non‐targeting (NT) siRNA. Representative images (B) of YFP-LC3 puncta (green) and DAPI nuclei staining (blue) are shown. (C) Upper panel: western blotting showed increased LC3‐II expression in HT29 cells with β‐catenin knockdown compared to control cells after 2 h starvation. Lower panel: quantification of the LC3‐II/β‐actin ratio by densitometry (mean±s.e.m. of four independent treatments, *P=0.011 in nutrient conditions, *P=0.035 under starvation)."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(D and E) HCT116 β‐cateninWT/− cells overexpressing a control or β‐cateninS33Y plasmid nutrient starved for 24 h. (D) Immunostaining of LC3 (green) with DAPI nuclei staining (blue) showed decreased LC3 puncta with β‐cateninS33Y overexpression. (E) By western blotting, LC3‐II decreased with β‐cateninS33Y overexpression (upper panel). Lower panel: quantification of the LC3‐II/β‐actin ratio by densitometry (mean±s.e.m. of four independent treatments, *P=0.015)."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(F) Comparison of isogenic β‐cateninWT/− and β‐catenin−/ΔS45HCT116 cells. Upper panel: western blotting showed decreased LC3‐II in HCT116β‐catenin−/ΔS45 cells. Lower panel: western blotting confirmed by quantification using densitometry of the LC3‐II/β‐actin ratio (mean±s.e.m. of three independent treatments, *P=0.046).Source data for this figure is available on the online supplementary information page."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(A) Protein expression of p62 increased following β‐catenin knockdown under normal and starved conditions in HT29 cells."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(B) Comparison of HCT116 expression in isogenic HCT116β‐cateninWT/− and HCT116β‐catenin−/ΔS45 cells by western blotting showed decreased p62 expression in β‐catenin−/ΔS45 cells."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(C) LC3 puncta number per cell during starvation and nutrient addback post starvation. Left panel: LC3 puncta number reduces in non‐targeting (NT) siRNA control with nutrient addback. Right panel: LC3 puncta number reduces in β‐catenin knockdown cells with nutrient addback (mean±s.e.m. of three independent treatments; n&gt;200 cells in &gt;20 fields of view per experiment). Western blotting for β‐catenin confirmed knockdown."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(D) Western blotting of LC3 and p62 in HT29 cells following β‐catenin knockdown and starvation with lysosomal inhibitors. LC3‐II and p62 protein expression showed an increase with β‐catenin siRNA in the presence of autophagy flux inhibitors chloroquine (10 μM) or bafilomycin A1 (100 nM; applied for the final 30 min of the 8 h) compared to β‐catenin knockdown alone. Quantification by densitometry of the LC3‐II/β‐actin ratio (mean±s.e.m. of four independent experiments). See also Supplementary Figure S1A.Source data for this figure is available on the online supplementary information page."
NCBI gene IDs:
"β‐catenin: 1499"
Figure legend: "(A-D) LC3 staining (red) in mouse intestinal crypt and villus epithelium increased (white arrowheads) 2 days post β‐catenin deletion in β‐catenin−/lox‐villin‐creERT2 mice (C and D) compared to control β‐catenin+/lox‐villin‐creERT2 mice (A and B). Further magnification of the areas marked by white squares is shown (lower panels)."
NCBI gene IDs:
"cre: 
β‐catenin: 12387
ERT2: 13983"
Figure legend: "(E-H)p62 staining (green) increased in the crypt and villus epithelium2 days after β‐catenin deletion in β‐catenin−/lox‐villin‐creERT2 mice (G and H) compared to control in β‐catenin+/lox‐villin‐creERT2 mice (E and F). DAPI staining (blue) identifies nuclei. β‐Catenin deletion was confirmed by immunofluorescence ( Supplementary Figure S1B). Red and green channel levels were adjusted post acquisition for clarity (equal level changes applied across the entire figure). Further magnification of the areas marked by white squares is shown (lower panels)."
NCBI gene IDs:
"cre: 
β‐Catenin: 12387
β‐catenin: 12387
ERT2: 13983"