EMBO/SourceData huggingface dataset:
Figure legend: "(F) Combined inactivation of endocytosis and kinesin‐mediated intracellular trafficking does not enhance protection of touch receptor neurons against MEC‐4(d)‐induced degeneration. Error bars denote s.e.m. values (n&gt;250 for all populations examined; P0.001, compared with control animals, unpaired t‐test)."
NCBI gene IDs:
"MEC‐4: 181728"
Figure legend: "Genetic interaction between kinesin‐mediated intracellular trafficking and cellular proteolytic pathways mediating necrotic cell death. Dysfunction of either kinesin 1 heavy chain (UNC‐116) or the monomeric kinesin UNC‐104 does not significantly enhance suppression of neurodegeneration in aspartyl protease‐deficient mutant animals (A, B), in animals with compromised lysosomal acidification (C, D), or in calpain protease‐deficient mutants animals (E, F). Error bars denote s.e.m. values (n&gt;250 for all populations examined; P&gt;0.5, compared with single mutant control animals, unpaired t‐test)."
NCBI gene IDs:
"UNC‐104: 174144
UNC‐116: 176179"
Figure legend: "(A) Kinesin 1 heavy chain (UNC‐116) deficiency."
NCBI gene IDs:
"UNC‐116: 176179"
Figure legend: "(B) Monomeric kinesin UNC‐104 deficiency. In both cases, no significant synthetic protection of neurons is observed in LGG‐1/LC3‐depleted animals with impaired autophagy. Error bars denote s.e.m. values (n&gt;250 for all populations examined; P&gt;0.5, compared with single mutant control animals, unpaired t‐test)."
NCBI gene IDs:
"LGG‐1: 174050
LC3: 177989///174050
UNC‐104: 174144"
Figure legend: "Chromogenic WM-ISH of miR-9 using miR-9 LNA 5'-Dig observed at different stages during development; longitudinal view, anterior to the left."
NCBI gene IDs:
"miR-9: 100033553"
Figure legend: "Transverse section of double fluorescent WM-ISH for her6 (green) and mir-9-4 (magenta) imaged in fixed embryo at 31hpf; scale bar 30μm."
NCBI gene IDs:
"mir-9-4: 100033555"
Figure legend: "Representative examples of triple fluorescent whole mount WM-ISH labelling of gfap (green), elavl3 (magenta) and her6 (grey) domains of expression (top panels) in hindbrain rhombomere6 (r6) in wild-type embryo observed at 34hpf; merged images indicate how the her6 expression domain overlaps with the progenitor zone (NP=gfap(+)/elavl3(-)), transition zone (T=gfap(+)/elavl3(+)) and neurogenic zone (N=gfap(-)/elavl3(+)); (bottom-right panel) schematic representation showing the her6 domain spanning the NP, T and N zones with quantification of distances from dorsal; transversal view; annotations denote dorsal (D) and ventral (V); scale bar 30μm."
NCBI gene IDs:
"elavl3: 30732
gfap: 30646
her6: 30288"
Figure legend: "Single molecule fluorescent in situ hybridization (smFISH) showing her6 (green), elavl3 (magenta) and dapi nuclear staining (blue) obtained from hindbrain (r6) sections of wild type embryo at 34hpf; head arrows indicates examples of co-existence of transcriptional active sites for her6 and elavl3; scale bar 3μm."
NCBI gene IDs:
"elavl3: 30732
her6: 30288"
Figure legend: "Triple fluorescent WM-ISH for gfap (green) and elavl3 (magenta) and her6 (grey) in the hindbrain (r6) of CTRL (top) and MBSm (bottom) embryos at 52hpf; dorsal (D); ventral (V); scale bar 30μm. Magnification of inset from merged gfap/elavl3 in (c) showing CTRL (top) and MBSm (bottom) embryos with corresponding NP/T/ N and NP/T zones respectively; dorsal (D); ventral (V); scale bar 30μm. Normalized intensity mean of elavl3 and gfap along the DV axis spanning the NP/T/N zones in CTRL and NP/T zones in MBSm respectively; region of interest (ROI) delineates high elavl3 versus gfap in CTRL (T and N zones) while in MBSm only the T zone is observed, overlap of elavl3 and gfap intensity mean peaks."
NCBI gene IDs:
"elavl3: 30732
gfap: 30646
her6: 30288"
Figure legend: "Normalized mean of elavl3 and gfap intensities in ROI observed in: (f) uninjected (UI) (5 embryos, 62 slices), (g) control (CTRL) (5 embryos, 35 slices) and (h) MBSm (8 embryos, 62 slices) conditions; bars represent mean and SEM; multiple t-test with Benjamini, Krieger and Yekuteli discovery, significance: p&lt;0.05*, p&lt;0.001***, p&lt;0.0001****."
NCBI gene IDs:
"elavl3: 30732
gfap: 30646"
Figure legend: "Transverse sections of double fluorescent WM-ISH for gfap (green) and neuroD4 (blue) in the hindbrain (r6) of CTRL (top) and MBSm (bottom) embryos at 52hpf; dorsal (D); ventral (V); scale bar 30μm."
NCBI gene IDs:
"gfap: 30646
neuroD4: 266958"
Figure legend: "Chromogenic WM-ISH showing neuroD4 expression intensity in: uninjected (UI) 51/56 embryos (91%), CTRL 50/54 embryos (93%) and mutant (MBSm); 36/65 embryos (55 %); longitudinal view, scale bar 100μm."
NCBI gene IDs:
"neuroD4: 266958"
Figure legend: "C Representative images and quantitative analyses of ACE2 immunohistochemical staining in lungs of age-matched wild type and G3 Terc-/- mice (n = 3-4 mice per group). Scale bar, 200 µm."
NCBI gene IDs:
"Terc: 21748"
Figure legend: "D Double-marker immunofluorescence and quantitative analyses of ACE2 intensity level in type II pneumocytic pro-SP-C-positive and CD31-positive endothelia in lungs of age-matched wild type and G3 Terc-/- mice (n = 3-4 mice per group). Scale bar, 100 µm. a.u. = arbitrary units."
NCBI gene IDs:
"Terc: 21748"
Figure legend: "A Immunofluorescence showing gammaH2AX foci in MEFs Trf2F/F at the indicated time points following 4OHT treatment and consequent TRF2 knockout. Scale bar, 25 µm. B RT-qPCR detection of Ace2 mRNA expression levels in MEFs Trf2F/F treated as in A (n = 3 independent experiments)."
NCBI gene IDs:
"Ace2: 70008
Trf2: 21750
TRF2: 21750"
Figure legend: "C Immunofluorescence showing gammaH2AX foci in HeLa shTRF2 cells at the indicated time points following doxycycline treatment and consequent TRF2 knockdown. Scale bar, 25 µm. D RT-qPCR detection of ACE2 mRNA expression levels in HeLa shTRF2 cells treated as in C (n = 6 independent experiments)."
NCBI gene IDs:
"ACE2: 59272
TRF2: 7014"
Figure legend: "E Representative immunofluorescence images of 53BP1 staining in liver from Trf2F/F mice treated with tamoxifen (to induce TRF2 loss and telomere uncapping) or vehicle. The animals as been injected also with PBS. Scale bar, 10 µm. F RT-qPCR detection of Ace2 mRNA expression levels in livers of mice treated as in E (n = 5-7 mice per group)."
NCBI gene IDs:
"Ace2: 70008
Trf2: 21750
TRF2: 21750"
Figure legend: "B Relative luciferase activity in HeLa shTRF2 following ionizing radiation (5 Gy) or TRF2 knockdown upon doxycycline-induced shTRF2 expression (n=3 independent experiments). Error bars represent the s.e.m. ﻿*P&lt;0.05. Two-way paired ANOVA."
NCBI gene IDs:
"TRF2: 7014"
Figure legend: "A Immunofluorescence showing gammaH2AX foci in Trf2F/F MEFs at the indicated time points following 4OHT treatment and consequent TRF2 knockout and treated with DMSO or ATMi. Scale bar, 25 µm. B RT-qPCR detection of Ace2 mRNA expression levels in MEFs Trf2F/F treated as in A (n = 3 independent experiments)."
NCBI gene IDs:
"Ace2: 70008
Trf2: 21750
TRF2: 21750"
Figure legend: "C Representative immunofluorescence images of 53BP1 staining in liver from Trf2F/F mice treated with tamoxifen (to induce telomere uncapping) or vehicle and injected with the indicated ASOs or PBS as control. Scale bar, 10 µm. D RT-qPCR detection of Ace2 mRNA levels in livers of mice treated as in C (n = 5-8 mice per group)."
NCBI gene IDs:
"Ace2: 70008
Trf2: 21750"
Figure legend: "E Representative microphotographs and quantitative analyses of ACE2 immunohistochemical staining in lungs of age-matched wild type and G3 Terc-/- mice, treated with the indicated ASOs (n = 4-9 mice per group). Scale bar, 200 µm."
NCBI gene IDs:
"Terc: 21748"
Figure legend: "F Double-marker immunofluorescence and quantitative analyses of ACE2 intensity level in type II pneumocytic pro-SP-C-positive cells in lungs of age-matched wild type and G3 Terc-/- mice, treated with the indicated ASOs (n = 4-9 mice per group). Scale bar, 100 µm. a.u. = arbitrary units."
NCBI gene IDs:
"Terc: 21748"
Figure legend: "(d) Parkin was overexpressed in young and senescent MEFs using adenovirus-mediated transduction for the in vitro cell-based bioassay to assess the ability to clear damaged mitochondria. Mitochondrial content after 24 h of treatment with 20 μM CCCP was assessed by flow cytometry for MitoTracker Green FM. Results from four independent experiments performed in duplicate are shown. MEFs at passage 3 and passage 9 were used as young and senescent MEFs, respectively."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "(g) Representative images of GFP-LC3 expressing MEFs treated with 20 μM CCCP for 10 h before the immunostaining of mitochondria with anti-TOM20 (red); original magnification, × 400 and × 1,000; scale bar, 100 μm and scale bar, 15 μm. Line scans below the images indicate colocalization between LC3 (green) and mitochondria (red) and correlate to the arrows drawn in the images. WHL indicates whole-heart lysate. Data are shown as the means±s.d. *P0.05; **P0.01 (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"LC3: 67443///66734"
Figure legend: "(a) The senescence-associated molecules p53, p21, p16, H-Ras 12V and Rb were overexpressed in MEFs by retrovirus-mediated transduction. After puromycin selection, mitophagy induction was performed with treatment of 20 μM CCCP 24 h after adenovirus-mediated Parkin expression. Mitochondrial content 24 h after mitophagy induction was assessed by flow cytometry for MitoTracker Green FM. Results are shown from four independent experiments performed in duplicate."
NCBI gene IDs:
"p21: 12575
p16: 12578
H-Ras: 15461
Parkin: 50873
Rb: 19645
p53: 22059"
Figure legend: "(c) Mitochondrial content of H-Ras 12 V expressing WT, p53−/− and p21−/− MEFs was assessed by flow cytometry 24 h after mitophagy induction. Results are shown from four independent experiments performed in duplicate."
NCBI gene IDs:
"p21: 12575
H-Ras: 15461
p53: 22059"
Figure legend: "(d) Mitochondrial content of WT and p53−/− MEFs was assessed by flow cytometry 24 h after mitophagy induction. WT and p53−/− MEFs were transfected with control or p53-specific siRNAs or cultured in complete medium for 24 h in the presence or absence of 20 μM pifithrin-α before mitophagy induction. Results are shown from four independent experiments performed in duplicate."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(e) Murine HL-1 cardiac myocytes were infected with retrovirus vectors encoding p53, p21 or p16 and after puromycin selection, underwent mitophagy induction. Mitochondrial content is assessed by flow cytometry from four independent experiments performed in duplicate are shown."
NCBI gene IDs:
"p21: 12575
p16: 12578
p53: 22059"
Figure legend: "(f) HL-1 cells were transfected with control, p53, p21 or p16-specific siRNAs, followed by treatment with 0.02 μM DOX and mitophagy induction. Mitochondrial content is assessed by flow cytometry from four independent experiments performed in duplicate."
NCBI gene IDs:
"p21: 12575
p16: 12578
p53: 22059"
Figure legend: "(g) CCCP-induced recruitment of Parkin and expression of Mfn1 and Drp1 in DOX-treated WT, p53−/− and p21−/− mice were determined by immunoblotting of the heartmitochondria-rich fraction. Representative immunoblots are shown from four independent experiments. Data are shown as the means±s.d. *P0.05; **P0.01 (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"p21: 12575
p53: 22059"
Figure legend: "(a) Whole-cell lysates of HL-1 cells treated with 0.02 μM DOX and transfected with FLAG-tagged Parkin or the FLAG-empty vector were immunoprecipitated with the anti-Flag-M2 antibody and blotted with anti-p53 and Flag antibodies."
NCBI gene IDs:
"Parkin: 50873
p53: 22059"
Figure legend: "(d) Schematic representation of N-terminally FLAG-tagged WT Parkin and various mutants of Parkin. (e) The association of endogenous p53 with various Parkin mutants in DOX-treated HeLa cells. The immunoprecipitation assay revealed that the RING0 domain was essential for the interaction with p53."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "(f) Schematic representation of the GST-p53 fusion proteins. (g) GST pull-down assays using GST-p53 fusion proteins and in vitro transcribed/translated FLAG-Parkin. Residues 81-160 of p53 were sufficient to bind Parkin."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(a) HL-1 cells transfected with control or p53-specific siRNAs no.1 and cultured in complete medium for 24 h in the presence or absence of 0.02 μM DOX. After treatment with 20 μM CCCP for 6 h, the fractionation experiment was performed. Endogenous Parkin translocation to mitochondria and ubiquitination were assessed by immunoblotting. Representative immunoblots are shown from three independent experiments."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(c,d) Representative images of YFP-Parkin overexpressing p53−/− HCT116 cells re-transfected with WT p53 and various mutants of p53 and treated with 60 μM CCCP for 8h (c) and 36 h (d) before the immunostaining of mitochondria with anti-TOM20 (red) and endogenous poly-ubiquitin with a specific antibody FK-2 (white); original magnification, × 630; scale bar, 20 μm."
NCBI gene IDs:
"Parkin: 5071
p53: 7157"
Figure legend: "(g) Maximum dP/dt was examined using cardiac catheterization with graded dobutamine infusion in WT and Parkin Tg aged littermates (n=8 per group)."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "ivMCs induce differentiation of co-cultured intestinal organoids as determined by qRT-PCR for stem cell (Lgr5, Olfm4) and proliferation (Ki67, Pcna) and differentiation markers (Muc2-goblet cells, Krt20-differentiated epithelial cells, Sim and Alpi-enterocytes). Enhanced terminal enterocytic differentiation was checked by villus center markers (Fabp1, Fabp2, G6pc, Leap2) and villus tip markers (Ada, Lama3, Lgals3, Cdh1). (Expression levels normalized to Ubiquitin B (Ubb), ENR parallel set as 1, error bars show sd, each dot represents one technical replicate of one representative experiment). If n = 2 technical replicates, error bars, and mean are not shown."
NCBI gene IDs:
"Ada: 11486
Alpi: 76768
Cdh1: 12550
Fabp1: 14080
Fabp2: 14079
G6pc: 14377
Krt20: 66809
Lama3: 16774
Leap2: 259301
Lgals3: 16854
Lgr5: 14160
Ki67: 17345
Muc2: 17831
Olfm4: 380924
Pcna: 18538
Sim: 69983
Ubb: 22187
Ubiquitin B: 22187"
Figure legend: "Relative expression of differentiation (Muc2-goblet cells, Krt20-differentiated epithelial cells, Sim and Alpi-enterocytes) and stem (Lgr5, Olfm4) markers in intestinal crypts upon 96 hours cultivation with indicated Bmp (500 ng/ml). (qRT-PCR, expression levels normalized to Ubiquitin B (Ubb), error bars show sd, Untreated parallel set as 1, each dot represents one technical replicate of one representative experiment.)."
NCBI gene IDs:
"Alpi: 76768
Krt20: 66809
Lgr5: 14160
Muc2: 17831
Olfm4: 380924
Sim: 69983
Ubb: 22187
Ubiquitin B: 22187"
Figure legend: "Bmp2 treatment induces the expression of the villus tip markers Nt5e (Cd73 protein). (Graph shows normalized counts determined by RNAseq, n= at least 3 for each treatment, timepoint: 24 hours, error bars denote sd)."
NCBI gene IDs:
"Cd73: 23959
Nt5e: 23959"
Figure legend: "Changes in the expression of villus tip and villus center genes after LDN-193189-4HCl (150 mg/kg) treatment determined by qRT-PCR normalized to β-actin (β-act). (Each dot represents one technical replicate of one representative experiment, error bars show sd.)."
NCBI gene IDs:
"β-act: 11461
β-actin: 11461"
Figure legend: "Induction of shRNA against Smad4 upon the doxycycline (+Dox) treatment (3 days) followed by Bmp2 treatment for additional 24 hours. shRNA activation is visualised by the expression of coupled TdTomato (red), scale bar, 200 μm."
NCBI gene IDs:
"Smad4: 17128"
Figure legend: "Smad4 knock-down (shRNA-ON) alleviates Bmp2-induced differentiation marked by relative expression of villus tip genes. (qRT-PCR, expression levels normalized to Ubiquitin B (Ubb), each dot represents one technical replicate of one representative experiment, error bars show sd, untreated parallel set as 1, treatment as in 6A)."
NCBI gene IDs:
"Smad4: 17128
Ubb: 22187
Ubiquitin B: 22187"
Figure legend: "Long term (4 weeks) organoid cultivation in the presence of Bmp2 (10 ng/ml) enriches the expression of villus tip genes. (qRT-PCR, expression levels normalized to Ubiquitin B (Ubb), each dot represents one technical replicate of one representative experiment, error bars show sd, untreated parallel set as 1, P2 - collected 4 days after 2nd passage, P4 -collected 4 days after 4th passage)."
NCBI gene IDs:
"Ubb: 22187
Ubiquitin B: 22187"
Figure legend: "qRT-PCR for villus tip genes and villus center genes in freshly established organoids cultivated 24 or 96 hours with Bmp2 (500 ng/ml), Wnt5a (500 ng/ml) or both Bmp2 + Wnt5a (each 500 ng/ml). Whereas Bmp2 induces villus tip genes by its own, it can act synergistically with Wnt5a. For the short-term cultivation, the effect is specific for villus tip genes (notice the different scale in left graphs). (Expression levels normalized to Hmbs, untreated parallel set as 1, each dot represents one technical replicate of one representative experiment, error bars show sd). If n = 2 technical replicates, error bars and mean are not shown."
NCBI gene IDs:
"Hmbs: 15288"
Figure legend: "Relative  mRNA levels of the  sAPX, 2-Cys Prx, GPXL, and PrxQ genes in response to  BSMV infections at 1, 3 and 5 dpi. The empty vector (EV) was used as a negative control, NbEF1α was used as an internal control. Data information: In (D), error bars indicate mean ± SEM from three independent experiments; * p-value &lt; 0.05, ** p-value &lt; 0.01, *** p-value &lt; 0.001, **** p-value &lt; 0.0001 from Student's t test."
NCBI gene IDs:
"sAPX: 
2-Cys Prx: 
EF1α: 
GPXL: 
PrxQ: "
Figure legend: "BSMV  accumulation in  2-Cys Prx     or GPXL-silenced  N. benthamiana plants using anti-TGB1  antibody. Empty vector TRV: 00 was used as negative control. Data information: representative data are shown, at least three independent replicates were performed with similar results."
NCBI gene IDs:
"2-Cys Prx: 
GPXL: "
Figure legend: "Co-localization of γb and NbNTRC during BSMV infection. NbNTRC-mCherry was co-expressed with BSMVγb-GFP (Jiang et al., 2020) in N. benthamiana, and confocal analyses were conducted at 3 dpi, and  chloroplast autofluorescence is displayed as a false-blue color. Scale bars, 20 μm."
NCBI gene IDs:
"GFP: 
mCherry: 
NTRC: 
γb: 962674"
Figure legend: "Analysis of VSR  activities of  γb  mutants. Infiltrated N. benthamiana leaves co-expressing sGFP  plus γbH85A-3xFlag,  γbH85C-3xFlag or the pGD empty vector (EV) were observed at 5 dpi under long-wave UV illumination for GFP fluorescence. The γb-3xFlag protein served as a positive control. The white dashed circles indicate the infiltrated zone.   Western blot  analysis of the  GFP protein in co-infiltrated  leaves at 5 dpi with anti-GFP  antibody. RbcL  was used as loading control.  Data information:  representative data were shown, and three independent experiments showed a similar result."
NCBI gene IDs:
"Flag: 
sGFP: 
γb: 962674"
Figure legend: "Effects of  NbNTRC overexpression on BSMV protein accumulation. N. benthamiana leaves  were first infiltrated with Agrobacterium containing various contructs indicated above the panel. After 1.5 dpi, BSMV was inoculated onto the leaves by agroinfiltration. Three days later, total protein was extracted for Western blotting with anti-TGB1 and anti-Flag  antibodies. The uncleaved NTRC with cTP and cleaved NTRC without cTP are marked with black and gray arrows RbcL was a loading control. Data information: representative data are shown and three eplicates similar results."
NCBI gene IDs:
"NTRC: "
Figure legend: "Effects of  NbNTRC  overexpression on BSMV replication. Movement-deficient  BSMV (RNAα +  RNAγ) was infiltrated into  leaves  that had been agroinfiltrated previously with various constructs indicated above the panel. NbNTRC and viral  γb proteins were detected by  Western blotting with anti-Flag and anti-γb antibodies. Uncleaved  NTRC with cTP and cleaved NTRC without  cTP are marked with black and gray arrows (see Appendix Fig S4).   RT-qPCR analyses of viral RNA accumulation in 4B. NbPP2A was used as an internal control.   Data information: representative data are shown and three eplicates similar results. In (C), error bars indicate mean ± SEM from three independent experiments."
NCBI gene IDs:
"NTRC: 
PP2A: 
RNAα: 
RNAγ: "
Figure legend: "Left panel: systemic symptoms of N. benthamiana  plants inoculated with BSMV and BSMV γb  mutants. Photographs were taken at 7 dpi. Right panel: schematic representation of inoculated (ino.), older systemic  leaves  (o-SL), and younger systemic leaves (y-SL) in N. benthamiana plants. Scale bars, 2 cm."
NCBI gene IDs:
"γb: 962674"
Figure legend: "Western blot analyses of TGB1 and CP accumulation in BSMV and BSMV γb mutant inoculated N. benthamiana leaves. Anti-TGB1 and anti-CP were used for protein detection. Data information: , representative data are shown, and three replicates had similar results. RbcL served as loading control."
NCBI gene IDs:
"γb: 962674"
Figure legend: "Effects of  NbNTRC overexpression on infection of BSMV  and BSMV γb mutants. A. tumefaciens harboring the indicated plasmids were infiltrated into N. benthamiana leaves. Note: Agrobacterium containing NbNTRC constructs were diluted 5-fold compared to normal use (OD600 = 0.3). BSMV and the γb mutants were then agroinfiltrated into the same  leaves  at 1.5 dpi. Three days later, Western blots were conducted with anti-TGB1 and anti-Flag antibodies. Uncleaved NTRC with cTP and cleaved  NTRC  without cTP  are marked with black and gray arrows Data information: representative data are shown, and three replicates had similar results. RbcL served as loading control."
NCBI gene IDs:
"NTRC: 
γb: 962674"
Figure legend: "Left panel: Western blot  detection of  BSMV CP accumulation in N. benthamiana leaves inoculated with  BSMV and γb movement deficient mutants. Leaf  samples were collected from different regions of the same leaf (right panel) Data information: representative data are shown, and three replicates had similar results. RbcL  served as loading control."
NCBI gene IDs:
"γb: 962674"
Figure legend: "Effects of γb and γb  mutants on 2-Cys Prx   in  N. benthamiana. Different combinantions of the pGD empty vector (EV) or γb and γb mutants are indicated above the panel. NbNTRC  and γb proteins were detected by Western blotting with anti-Flag  and anti-γb  antibodies under reducing conditions, Western blot analyses of 2-Cys Prx  abundance with anti-2-Cys Prx  antibody under non-reducing conditions. The uncleaved NTRC  with cTP and cleaved  NTRC without  cTP are marked with black and gray arrows, respectively. Data information: representative data are shown, and three replicates had similar results.  RbcL served as loading control."
NCBI gene IDs:
"γb: 962674"
Figure legend: "Split-LUC assays to determine NbNTRC-2-Cys Prx interactions in N. benthamiana during BSMV infection. NbNTRC-cLUC  and 2-Cys Prx-nLUC  were co-expressed in N. benthamiana leaves  infected with the indicated BSMV derivatives, Bioluminescence  was observed at 3 dpi. The white dashed circles indicate the infiltrated regions.   Quantification of the luciferase signals shown in Figure 6C as assessed by ImageJ software.   Data information: Representative data were shown , and three replicates had similar results. In (D), error bars indicate mean ± SEM (n = 6). c indicate statistically significant differences among different groups (ANOVA, p-value &lt; 0.05)."
NCBI gene IDs:
"2-Cys Prx: 
LUC: 
NTRC: "
Figure legend: "Western blot  analyses to detect LRSV γb in NbNTRC-OE and NbNTRC-KO N. benthamiana  plants challenged with LRSV at 8 dpi. RbcL  was used as the loading control. Data information: representative data are shown, and three replicates had similar results."
NCBI gene IDs:
"NTRC: "