EMBO/SourceData huggingface dataset:
Figure legend: "(I-J) Representative traces showing typical responses of PVH CRH neurons to water spay (arrows) in NachBac mice (I), and comparison of responsiveness to water spray between NachBac and control mice (J).Gray traces showed Ca2+ independent signal for system stability and red ones showed Ca2+ dependent signals for neuron activity, N=5-7 each, males, data presented as mean +/-SEM, **p&lt;0.0001 in both (H and J), unpaired Student's t tests."
NCBI gene IDs:
"NachBac: "
Figure legend: "CRH-Cre male mice received stereotaxic delivery of AAV-EF1a-Flex-EGFP-P2A-mNachBac or control GFP vectors at 7-8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. Weekly body weight gain after viral delivery (A) at 11 weeks after viral delivery."
NCBI gene IDs:
"EF1a: 
EGFP: 
GFP: 
mNachBac: 
P2A: 
Cre: 2777477
CRH: 12918"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of AAV-EF1a-Flex-EGFP-P2A-mNachBac or control GFP vectors at 7-8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. measurements of fat mass (B) and lean mass (C) at 11 weeks after viral delivery."
NCBI gene IDs:
"EF1a: 
EGFP: 
GFP: 
mNachBac: 
P2A: 
Cre: 2777477
CRH: 12918"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of AAV-EF1a-DIO-Kir2.1-P2A-dTomato vectors and AAV-Flex-GFP at 7-8 weeks of age and recording was made at least 4 weeks after viral delivery on neurons with Kir2.1 (one side) or control GFP neurons (contralateral side) of the same animal (A)"
NCBI gene IDs:
"dTomato: 
EF1a: 
GFP: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "typical spontaneous neuron activity recorded in GFP controls (B, top panel) and Kir2.1 neurons (B, bottom); and comparison in resting membrane potentials between the two groups (C, **p&lt;0.0001, unpaired Student's t test)."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "CRH-Cre male mice were injected with either AAV-Flex-mCherry or AAV-EF1a-DIO-Kir2.1-P2A-dTomato to examine c-Fos expression in the PVH in 24hr fasting or refed after fasting. (D) Representative images showing CRH neurons expressing mCherry or Kir2.1 (mCherry in the top 2 rows and Kir2.1 in the bottom 2 rows of the second colomn), c-Fos expression (green in the first colomn), merged of red and green (the third colomn) and the amplied pictures for the boxed areas in the merged images (the forth colomn). The experimental conditions for each group (fasting verus refed) are labelled on the left."
NCBI gene IDs:
"dTomato: 
EF1a: 
mCherry: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "CRH-Cre male mice were injected with either AAV-Flex-mCherry or AAV-EF1a-DIO-Kir2.1-P2A-dTomato to examine c-Fos expression in the PVH in 24hr fasting or refed after fasting. (E-F) Comparison of average neuron numbers from cell counting on total number of c-Fos positive neurons in the PVH (E, **p=0.0001: fasting versus refed; *p=0.0497: refed versus refed/Kir2.1; **p=0.0025: fasting/Kir2.1 versus refed Kir2.1) and total number of c-Fos positive CRH neurons (F, **p&lt;0.0001: fasting versus refed; p=0.9586: fasting/Kir2.1 versus refed/Kir2.1), n=3-4 each, two way ANOVA tests. All data presented as mean +/- SEM."
NCBI gene IDs:
"dTomato: 
EF1a: 
mCherry: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of a mixture of AAV-Flex-GCaMP6m and AAV-EF1a-DIO-Kir2.1-P2A-dTomato vectors at 7-8 weeks of age with optic fiber implantation targeting PVH neurons. (G-H) Representative traces showing typical responses of PVH CRH neurons to water spay (arrows) in Kir2.1 mice (G), and comparison of responsiveness to water spray between Kir2.1 and control mice (H)."
NCBI gene IDs:
"dTomato: 
EF1a: 
GCaMP6m: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of a mixture of AAV-Flex-GCaMP6m and AAV-EF1a-DIO-Kir2.1-P2A-dTomato vectors at 7-8 weeks of age with optic fiber implantation targeting PVH neurons."
NCBI gene IDs:
"dTomato: 
EF1a: 
GCaMP6m: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of AAV-EF1a-DIO-Kir2.1-P2A-dTomato or control AAV-Flex-mCherry vectors at 7-8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. Weekly body weight gain after viral delivery (A, *p&lt;0.0001, unpaired Student's t test), at 15 weeks after viral delivery."
NCBI gene IDs:
"dTomato: 
EF1a: 
mCherry: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "CRH-Cre male mice received stereotaxic delivery of AAV-EF1a-DIO-Kir2.1-P2A-dTomato or control AAV-Flex-mCherry vectors at 7-8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. measurements of fat mass (B, **p=0.0006, unpaired Student's t test) and lean mass (C, p=0.3728, unpaired Student's t test) at 15 weeks after viral delivery."
NCBI gene IDs:
"dTomato: 
EF1a: 
mCherry: 
P2A: 
Cre: 2777477
CRH: 12918
Kir2.1: 16518"
Figure legend: "Quantitative expression of Lif mRNA in the colon of DSS-challenged and control mice (n=6 per group)."
NCBI gene IDs:
"Lif: 16878"
Figure legend: "Quantitative expression of Lif mRNA in LPLs and IECs from DSS-challenged and control mice (n=4 per group)."
NCBI gene IDs:
"Lif: 16878"
Figure legend: "Quantitative expression of Lif mRNA in the colon of SPF mice treated with or without antibiotics (Abx) at the indicated time points during colitis induction (n=3 per group)."
NCBI gene IDs:
"Lif: 16878"
Figure legend: "Quantitative expression of Lif mRNA in LPLs and IECs from mice treated with or without Abx at the indicated time points during colitis induction (n=3 per group)."
NCBI gene IDs:
"Lif: 16878"
Figure legend: "Stat4 mRNA expression in LPLs and IECs (n=6)."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Weight loss of wild-type (WT) (n=6) or Stat4 knockout(Stat4-KO) (n=6) mice receiving intraperitoneal injection of PBS or LIF during challenge with 3% DSS."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "wild-type (WT) (n=6) or Stat4 knockout(Stat4-KO) (n=6) mice receiving intraperitoneal injection of PBS or LIF during challenge with 3% DSS. Macroscopic changes in the colon of the mice on day 10 of colitis induction Comparison of the colon length in the mice on day 10 of colitis induction"
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "wild-type (WT) (n=6) or Stat4 knockout(Stat4-KO) (n=6) mice receiving intraperitoneal injection of PBS or LIF during challenge with 3% DSS. DAI (stool consistency and bleeding score) of colitis mice treated"
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "wild-type (WT) (n=6) or Stat4 knockout(Stat4-KO) (n=6) mice receiving intraperitoneal injection of PBS or LIF during challenge with 3% DSS. H&amp;E histology of representative colons from colitis mice. Scale bar, 20μm."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Weight loss of Rag1-/- (n=6) mice transferred with CD45RBhi CD4+ T cells and intraperitoneally injected with PBS or LIF for 8 weeks."
NCBI gene IDs:
"Rag1: 19373"
Figure legend: "Rag1-/- (n=6) mice transferred with CD45RBhi CD4+ T cells and intraperitoneally injected with PBS or LIF for 8 weeks. Macroscopic changes in the colon of Rag1-/- mice treated Comparison of colon lengths in the Rag1-/- mice at week 8 (n=5)."
NCBI gene IDs:
"Rag1: 19373"
Figure legend: "Rag1-/- (n=6) mice transferred with CD45RBhi CD4+ T cells and intraperitoneally injected with PBS or LIF for 8 weeks. H&amp;E histology of representative mouse colons on day 54 of colitis induction"
NCBI gene IDs:
"Rag1: 19373"
Figure legend: "Colons obtained from WT or Stat4-KO mice on day10 of the colitis model induced as in Figure 2A were immunostained with an antibody against phosphorylated STAT4. Scale bar, 50μm."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "FACS staining of LPLs isolated on day10 from the colon of WT or Stat4-KO colitis mice receiving PBS or LIF (n=4 per group). The percentage of IL-17A+ and/or IFNγ+ CD4+ T cells in vivo was analyzed."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Quantitative expression of Il17a mRNA in MLNs and LNs from colitis mice treated"
NCBI gene IDs:
"Il17a: 16171"
Figure legend: "FACS staining of WT and Stat4-KO naïve CD4+ T cells treated with LIF (50 ng/ml) on day 4 of induction into Th17 cell subsets."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "qPCR analysis of Th17-related genes in WT or Stat4-KO naïve CD4+ T cells on day 4 of induction into Th17 cell subsets in the absence or presence of LIF (n=3 per group)."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "FACS staining of WT or Stat4-KO naïve CD4+ T cells treated with LIF (50 ng/ml) on day 4 of induction into nonpathogenic or pathogenic Th17 cells."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Cultured HeLa cells transfected with GFP-tagged STAT constructs were exposed to LIF and imaged 30 minutes later. Scale bar, 50μm."
NCBI gene IDs:
"GFP: 
STAT: 20848"
Figure legend: "Heatmap showing Th17 signature genes (left) or Th17 regulatory genes (right) up- or downregulated in WT or Stat4-KO LPLs by LIF treatment (n=2)."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Quantitative mRNA expression analysis of the indicated genes in WT or Stat4-KO naïve CD4+ T cells on day 4 of induction into Th17 cell subsets in the absence or presence of LIF."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "The GAS-luciferase reporter activity assay was performed in Stat3-/- MEFs transfected with empty vector (EV) or STAT4 constructs and then treated with LIF for 8 hours."
NCBI gene IDs:
"Stat3: 20848
STAT4: 20849"
Figure legend: "Relative SIE-(I) activities in Stat3-/- MEFs transfected with EV or the indicated STAT constructs and treated with LIF for 8 hours."
NCBI gene IDs:
"STAT: 20848
Stat3: 20848"
Figure legend: "Relative AGG-luciferase reporter activities in Stat3-/- MEFs transfected with EV or the indicated STAT constructs and treated with LIF for 8 hours."
NCBI gene IDs:
"STAT: 20848
Stat3: 20848"
Figure legend: "ChIP analysis of naïve CD4+ T cells obtained from IL-17A-eGFP mice cultured under Th0 or Th17 conditions in the absence or presence of LIF for 3 days. GFP+ cells isolated by flow cytometry were restimulated with IL-6 in the presence or absence of LIF, crosslinked with formaldehyde and immunoprecipitated with anti-STAT3 (top) or anti-STAT4 (bottom) antibodies, followed by the amplification of the immunoprecipitated DNA by quantitative PCR with the p1-p10 primer pairs. The results are presented relative to the amount of input DNA."
NCBI gene IDs:
"eGFP: 
IL-17A: 16171"
Figure legend: "ChIP analysis of naïve WT or Stat4-KO CD4+ T cells cultured under Th0 or Th17 conditions in the absence or presence of LIF for 3 days. The cells were restimulated with IL-6 or LIF for 30 minutes, crosslinked with formaldehyde and immunoprecipitated with the anti-STAT3 antibody, followed by the amplification of the immunoprecipitated DNA by quantitative PCR with the p1-p10 primer pairs. The results are presented relative to the amount of input DNA."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Relative Il17a-SIE- (left) or AGG-luciferase reporter (right) activities in Stat3-/- MEFs transfected with EV, STAT3 or STAT4 or cotransfected with STAT3 and STAT4 and then treated with IL-6 or LIF for 8 hours."
NCBI gene IDs:
"Il17a: 16171
Stat3: 20848
STAT3: 20848
STAT4: 20849"
Figure legend: "Relative Il17a promoter-luciferase reporter activities in Stat3-/- MEFs transfected with the indicated constructs and then treated with LIF for 8 hours."
NCBI gene IDs:
"Il17a: 16171
Stat3: 20848"
Figure legend: "Quantitative mRNA expression analysis of the indicated genes in colon tissues obtained from WT or Stat4-KO colitis mice treated"
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Immunoblot analysis of YAP expression in WT or Stat4-KO IECs treated with LIF (20 ng/ml) for different durations; β-actin was used as a loading control (top). The statistical analysis of YAP protein intensity is shown in the bottom panel."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "Relative YAP promoter-luciferase reporter activities in HEK293T cells transfected with EV, STAT3 or STAT4 and then treated with IL-6 or LIF for 8 hours."
NCBI gene IDs:
"STAT3: 20848
STAT4: 20849
YAP: 10413"
Figure legend: "16S rRNA sequencing analyses of the Proteobacteria distribution in feces from WT or Stat4-KO colitis mice. (n=5 per group)."
NCBI gene IDs:
"Stat4: 20849"
Figure legend: "qPCR of the 16S rRNA genes of microbes in the phylum Proteobacteria, family Enterobacteriaceae, and genera Escherichia-Shigella in the feces of the indicated mice on day0 and day10 (n=6 per group) of colitis induction."
NCBI gene IDs:
"16S rRNA: "
Figure legend: "B. Semi-quantitative PCR of miR-132 and negative control miRNAs in the hippocampus of antagomiR-132-injected mice (ant-132) in comparison to control-injected animals (aCSF and scramble) at six months of age. Sample size, n=9 per group."
NCBI gene IDs:
"miR-132: 387150"
Figure legend: "C. FISH of miR-132 and negative control miR-124 in the hippocampus of ant-132- and scramble-injected mice. Scramble probes were used as FISH negative controls. Scale bar, 500μm."
NCBI gene IDs:
"miR-124: 70441///268755///12797995
miR-132: 387150"
Figure legend: "D. Semi-quantitative PCR of miR-132 and miR-212 in the hippocampus of 3-month old miR-132 mimic-injected mice (miR-132) compared to animals injected with a negative control oligonucleotide (Ctr). Sample size, n=6 per group."
NCBI gene IDs:
"miR-132: 387150
miR-212: 387208"
Figure legend: "A. Amyloid staining (6E10) combined with miR-132FISH in ant-132- and scramble-injected mice at six months of age. Scale bar, 500 μm. B. Quantification of amyloid burden in hippocampus and cortex of ant-132-injected and control animals (see Materials and Methods). Sample size, n=4 per group."
NCBI gene IDs:
"miR-132: 387150"
Figure legend: "C, D. ELISA of soluble (TBS) and insoluble (Formic acid) Aβ40 and Aβ42 levels in the hippocampus of ant-132- (C) and miR-132-injected (D) animals at six and three months of age, respectively. Sample size, n=6 per group."
NCBI gene IDs:
"miR-132: 387150"
Figure legend: "Western blot analysis of total TAU and pTAU (AT8, AT270) levels upon miR-132 knockdown (A) or overexpression (B) at six and three months of age, respectively. Sample size, n=9 per group (A) and n=6 per group (B). Values were normalized to the respective control groups and presented as mean ± SEM. Student's t-test was used."
NCBI gene IDs:
"miR-132: 387150"
Figure legend: "A. Luciferasereporter assay of wild-type (wt) and mutant (mut) ITPKB 3'UTR in HEK293 cells co-transfected with a synthetic miR-132 (miR-132) or a negative control (Neg Ctr) oligonucleotide."
NCBI gene IDs:
"Luciferase: 
ITPKB: 3707
miR-132: 406921"
Figure legend: "B. ELISA of Aβ40 and Aβ42 levels in HEK293-APPswecells transfected with a miR-132 antisense oligonucleotide (miR-132 inh), an siRNA against ITPKB (ITPKB siRNA) or both. The assays in A and B were performed in three independent experiments, each in triplicates."
NCBI gene IDs:
"APP: 351
ITPKB: 3707
miR-132: 406921"
Figure legend: "C. Western blot analysis of ITPKB knock down in APPPS1hippocampus using an siRNA oligonucleotide against ITPKB. Sample size, n=6 per group. Values were normalized to control-injected group (Ctr) and presented as mean ± SEM."
NCBI gene IDs:
"APP: 351
ITPKB: 320404
PS1: 5663"
Figure legend: "D. ELISA of insoluble (Formic acid-soluble) Aβ40 and Aβ42 levels in the hippocampus of ITPKB siRNA- and control-injected animals at three months of age. Sample size, n=6 per group. Values were normalized to control group and presented as mean ± SEM."
NCBI gene IDs:
"ITPKB: 320404"
Figure legend: "E, F. Western blot analysis of ITPKB levels upon miR-132 down- (E) or up-regulation (F) at six and three months of age, respectively. Sample size, n=9 per group for miR-132 down regulation and n=6 per group for miR-132 overexpression. Values were normalized to control groups and presented as mean ± SEM."
NCBI gene IDs:
"miR-132: 387150"
Figure legend: "Western blot analysis of CTFβ (A) levels upon miR-132 down- (ant-132) (left panel) or upregulation (miR-132) (right panel) in APPPS1hippocampus at six and three months of age, respectively. Sample size, n=9 per group for miR-132 down regulation and n=6 per group for miR-132 overexpression. Values were normalized to respective control groups and presented as mean ± SEM. The Student's t-test was used."
NCBI gene IDs:
"APP: 351
miR-132: 387150
PS1: 5663"
Figure legend: "Western blot analysis of sAPPβ-swe (B) levels upon miR-132 down- (ant-132) (left panel) or upregulation (miR-132) (right panel) in APPPS1hippocampus at six and three months of age, respectively. Sample size, n=9 per group for miR-132 down regulation and n=6 per group for miR-132 overexpression. Values were normalized to respective control groups and presented as mean ± SEM. The Student's t-test was used."
NCBI gene IDs:
"APP: 351
miR-132: 387150
PS1: 5663"
Figure legend: "Western blot analysis of phosphorylated ERK1/2 (pERK1/2) (C) levels upon miR-132 down- (ant-132) (left panel) or upregulation (miR-132) (right panel) in APPPS1hippocampus at six and three months of age, respectively. Sample size, n=9 per group for miR-132 down regulation and n=6 per group for miR-132 overexpression. Values were normalized to respective control groups and presented as mean ± SEM. The Student's t-test was used."
NCBI gene IDs:
"APP: 351
miR-132: 387150
PS1: 5663"
Figure legend: "B. miR-132FISH coupled with double immunofluorescence against hyperphosphorylated TAU (AT8)-containing neurofibrillary tangles (NFTs) and ITPKB in AD prefrontal cortex. miR-124 was used as a control. Scale bar, 50 μm."
NCBI gene IDs:
"miR-124: 406907///406909///406908
miR-132: 406921"
Figure legend: "C. Quantification of miR-132, ITPKB and hyperphosphorylated TAU (pTAU) signal in single neurons. Integrated intensity values of each signal per cell were normalized to the mean integrated density of each signal across all the cells analyzed (see Materials and Methods). Sample size (AD patients), n=3. Values are presented as mean ± SEM. Two-way Anova was used. Quantifications are summarized in the table provided. Arrow directions refer to the comparison of each normalized signal to the same signal in the other group (in the \"low miR-132\" group comparisons are made to the \"high miR-132\" group and vice versa)."
NCBI gene IDs:
"miR-132: 406921"
Figure legend: "Semi-quantitative PCR of miR-132 and western blot analysis of ITPKB, phosphorylated (pERK1/2) and total ERK1/2, phosphorylated (AT8 , AT270) and total TAU, phosphorylated sphingosine kinase 1 (pSphK1), BACE1, full length APP (flAPP), APP CTFs and sAPPβ in human AD hippocampi (AD) compared to non demented control samples (ND). Sample size, n=39 for AD and n=15 for ND. Values were normalized to ND and presented as mean ± SEM. The Student's t-test was used."
NCBI gene IDs:
"ERK1: 
miR-132: 406921"
Figure legend: "GAG content as assessed by Alcian blue staining with spectrophotometric B C28/I2 micromasses treated with GCP-2 siRNA (n=12) or scrambled control siRNA (Scr; n=14); p values were determined by unpaired two-tailed Student's t test."
NCBI gene IDs:
"GCP-2: 10844"
Figure legend: "D qPCR for ACAN (Aggrecan) expression in C3H10T½ micromasses treated for 3 days with recombinant GCP-2 (100 ng/ml) (n=3) or vehicle (n=4); ACAN - Aggrecan;"
NCBI gene IDs:
"ACAN: 11595
Aggrecan: 11595"
Figure legend: "A, B qPCR for (A) Runx2 and (B) Col1A1 expression in HAC micromasses treated for 24 hours with recombinant GCP-2 (100 ng/ml) or vehicle (n=4); Runx2 = Runt-related transcription factor 2, Col1A1 = collagen type 1 alpha 1; n=4"
NCBI gene IDs:
"Col1A1: 1277
collagen type 1 alpha 1: 1277
Runx2: 860"
Figure legend: "E qPCR for Col10A1 (n=3) of HAC 3 weeks after treatment with vehicle, T3 (100 ng/ml) or T3+GCP-2 (100 ng/ml); n=3;"
NCBI gene IDs:
"Col10A1: 1300"
Figure legend: "D, E Maximum migratory activity of WT GCP-2 (50nM) was compared to GCP-2 mutants (50nM) in (D) chemotaxis and (E) trans-endothelial migration assays in CXCR2-expressing 300-19 pre B cells. WT = wildtype GCP-2; K101E = GCP-2_K101E single mutant; K105E = GCP-2_K105E single mutant; D = GCP-2_K101E_K105E double mutant; T = GCP-2_K100E_K101E_K105E triple mutant; n=4; p values were determined by fitting a generalised linear model followed by pairwise comparison of the estimated marginal means."
NCBI gene IDs:
"GCP-2: 10844"
Figure legend: "C28/I2 micromasses stimulated with WT GCP-2, GCP-2 triple mutant (GCP-2-T) or untreated and assessed for (F) AKT phosphorylation levels by western blotting; V = vehicle treated samples, GCP-2 = WT GCP-2 treated samples, GCP-2-T = triple mutant GCP-2 treated samples, pAKT = phosphorylated AKT, tAKT = total AKT. On the right, quantification of the density of the bands using ImageJ. Five independent experiments were analyzed. The data were scaled and p values were determined by fitting a generalised linear model followed by pairwise comparison of the estimated marginal means."
NCBI gene IDs:
"GCP-2: 10844"
Figure legend: "C28/I2 micromasses stimulated with WT GCP-2, GCP-2 triple mutant (GCP-2-T) or untreated and assessed G spectrophotometric quantification of proteoglycan content by Alcian blue staining. p values were determined by Kruskal-Wallis test with Dunn's post-hoc test; (n=8)."
NCBI gene IDs:
"GCP-2: 10844"
Figure legend: "C28/I2 micromasses stimulated with WT GCP-2, GCP-2 triple mutant (GCP-2-T) or untreated and assessed H ACAN expression levels by qPCR; ACAN = Aggrecan; p values were determined by ANOVA with Tukey's HSD post-hoc test; n=4."
NCBI gene IDs:
"ACAN: 176
Aggrecan: 176
GCP-2: 10844"
Figure legend: "I-J Real time quantitative PCR of COL2A1 (I) and COL10A1 (J) mRNA normalized to β actin. p values were determined by fitting a generalised linear model followed by comparison of the estimated marginal means; n=4 control, 5 GCP-2 and GCP-2-T for COL2A1 and n=6 per group for COL10A1."
NCBI gene IDs:
"β actin: 60
COL10A1: 1300
COL2A1: 1280
GCP-2: 10844"
Figure legend: "K Number of neutrophils counted within the intercondylar space on sections from mice killed 4 days after an intra-articular injection of adenovirus encoding GFP (n= 3), GCP-2 (n=3) and GCP-2-T (n=3); p values were determined by fitting a generalised linear model (family=poisson) followed by pairwise comparison of the estimated marginal means."
NCBI gene IDs:
"GFP: 
GCP-2: 74237"
Figure legend: "B Weekly pain measurement by incapacitance, shown as the percentage of bodyweight loaded on the operated leg in GFP (n= 15) vs GCP-2 (n=14) and GFP vs GCP-2-T (n=15) treated mice. Circles show the mean, and error bars show 95% confidence intervals."
NCBI gene IDs:
"GFP: 
GCP-2: 74237"
Figure legend: "C Incapacitance at week 10 (final time-point); red line indicates 50% loading (no pain); p values were determined by unpaired, two-tailed one sample Student's t test testing the hypothesis that mice loaded 50% body weight on the operated limb (n=13 in the GFP group and n=14 in the GCP-2 and GCP-2-T groups). D The area under the curve (AUC) of incapacitance calculated starting from week 6; p values were determined by ANOVA with Tukey's HSD post-hoc test (n=13 in the GFP group and 14 in the GCP-2 and GCP-2-T group)."
NCBI gene IDs:
"GFP: 
GCP-2: 74237"
Figure legend: "E, F Osteoarthritis severity assessed using (E) OARSI scoring system 10 weeks after MLI; GFP (n= 7), GCP-2 (n=8) and GCP-2-T (n=7); p values were determined by fitting a generalized linear model followed by comparison of the estimated marginal means. F representative image (Safranin O staining) for each treatment; arrows indicate the tidemark;"
NCBI gene IDs:
"GFP: 
GCP-2: 74237"
Figure legend: "G Quantification of osteophytes area; GFP (n= 7), GCP-2 (n=7) and GCP-2-T (n=8); H Representative images with osteophyte highlighted I Osteophyte maturity score (n=7); p values were determined by fitting a generalized linear model followed by pairwise comparison of the estimated marginal means."
NCBI gene IDs:
"GFP: 
GCP-2: 74237"
Figure legend: "Photograph of male patient with FMR1G-ins. allele. Typical physical and behavioral features of FXS were noted in the patient, who shows moderate to severe intellectual disability."
NCBI gene IDs:
"FMR1: 2332"