EMBO/SourceData huggingface dataset:
Figure legend: "(c) HCT116 cells were transfected with SIRT2 RNAi plasmid or an empty control plasmid. At 48 h after transfection, the cytosolic lysate was extracted for probing with SIRT2 or FoxO1 (upper panels), or for co-immunoprecipitation with anti-FoxO1, followed by probing with anti-acetylated lysine (lower panels). IB, immunoblot."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(a, b) H1299 cells were transfected with an empty control plasmid or with the Flag-FoxO1(WT) plasmid. At 24 h after transfection, the cytosolic lysate was extracted for co-immunoprecipitation with anti-FoxO1, followed by probing with anti-mTOR, Beclin 1, Vps34 or Atg1 (a) and with Atg3, Atg4, Atg10, Atg7 or LC3 (b)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(c) H1299 cells were co-transfected with Myc-Atg7, with Flag-FoxO1(WT) or with an empty control plasmid. At 24 h after transfection, the cytosolic lysate was extracted for Flag with anti-Flag, followed by probing with anti-Myc."
NCBI gene IDs:
"Atg7: 10533
FoxO1: 2308"
Figure legend: "(e) Different Myc-tagged FoxO1 fragments and Flag-tagged Atg7 were co-transfected into H1299 cells, and the cytosolic lysate was extracted for co-immunoprecipitation with anti-Myc, followed by probing with anti-Flag."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(f) HCT116 cells were transfected with an empty control plasmid, an SIRT2(WT)-expressing plasmid or an SIRT2(168A)-expressing plasmid. At 24 h after transfection, cells were serum-starved for 24 h, and the cytosolic lysate was extracted for co-immunoprecipitation with anti-FoxO1, followed by probing with anti-Atg7."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(a) HCT116 cells or stable FoxO1-RNAi HCT116 cells were treated with a non-specific RNAi control or SIRT2-RNAi, and LC3-II accumulation or p62 degradation was detected in the presence or absence of E64 and pepstatin A."
NCBI gene IDs:
"FoxO1: 2308
SIRT2: 22933"
Figure legend: "(b) HCT116 cells or stable FoxO1-RNAi HCT116 cells were treated with 10 μM AGK2 for 24 h, in the presence or absence of E64 and pepstatin A. Changes in FoxO1, p62 and LC3-II were determined by western blotting (upper panels), or the formation of LC3 punctate signals was observed (lower panels). HCT116 cells were treated with 10 μM AGK2 for 24 h and the formation of LC3 punctate signals was observed. Quantification of the ratio of LC3 punctate cells to overall HCT116 cells is shown in the lower right panel. Data are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(c) H1299 cells were transfected with Flag-FoxO1(WT), SIRT2(WT) or SIRT2(168A). At 24 h after transfection, the cell lysate was extracted and immunoblotted with anti-Flag, anti-SIRT2, anti-LC3, anti-p62 or anti-β-actin.."
NCBI gene IDs:
"FoxO1: 2308
SIRT2: 22933"
Figure legend: "(d) HCT116 cells were transfected with a control plasmid, a SIRT2(WT)-expressing plasmid or a SIRT2(168A)-expressing plasmid. At 24 h after transfection, the cells were subjected to serum starvation for 24 h. The cell lysate was extracted and immunoblotted with anti-SIRT2, anti-LC3, anti-p62 or anti-β-actin."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(e) H1299 cells were transfected with Myc-Atg7, Flag-FoxO1(WT) or mutated Flag-FoxO1(KR). At 24 h after transfection, the cytosolic lysate was extracted for co-immunoprecipitation with anti-Myc, followed by probing with anti-Flag."
NCBI gene IDs:
"Atg7: 10533
FoxO1: 2308"
Figure legend: "(f) H1299 cells were transfected with a control plasmid, Flag-FoxO1(WT), Flag-FoxO1(K262R), Flag-FoxO1(K265R), Flag-FoxO1(K274R) or Flag-FoxO1(KR). At 24h after transfection in the presence or absence of E64 and pepstatin A and immunoblotted, the cell lysate was extracted and immunoblotted with anti-Flag, anti-LC3, anti-p62 or anti-β-actin."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(g) Left: H1299 cells were co-transfected with GFP-LC3, Flag-FoxO1(WT) or Flag-FoxO1(KR). The formation of GFP-LC3-labelled punctate signals was observed 24 h after transfection. Right: quantification of the ratio of GFP-LC3-labelled punctate cells to overall survived H1299 cells. Data in g are means ± s.d. (n = 3). Scale bars, 25 μm (b), 10 μm (g)."
NCBI gene IDs:
"FoxO1: 2308
LC3: 440738///81631///84557"
Figure legend: "(a) H1299 cells were transfected with a control plasmid, Flag-FoxO1(WT), Flag-FoxO1(3A), Flag-FoxO1(ΔDB) or Flag-FoxO1(ΔDB3A), in the presence or absence of 10 mM 3-MA (upper panel) or in the presence or absence of 50 μM Z-VAD-fmk (lower panel); cells were stained with propidium iodide (PI) 48 h after transfection. PI-stained (dead) cells were counted by flow cytometry and the percentage cell loss was calculated."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(b) FoxO1(WT) or Flag-FoxO1(ΔDB) was transfected into H1299 cells; 48 h later the autophagic vesicles were observed under an electron microscope. Enlarged images (right) show clearer autophagic vesicles indicated by arrows. Scale bars, 2 μm."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(c) 48 h cells with or without Atg5 RNAi were transfected with FoxO1(WT), and cell death was determined by staining with PI at 48 h after transfection. Data in c are means ± s.d. (n = 3)."
NCBI gene IDs:
"Atg5: 9474
FoxO1: 2308"
Figure legend: "(d) The stable Atg5 knockdown H1299 cells was further transfected with a control plasmid, Flag-FoxO1(WT), or Flag-FoxO1(ΔDB), and the percentage cell loss was quantified by flow cytometry at 48 h after transfection. Data in d are means ± s.d. (n = 3)."
NCBI gene IDs:
"Atg5: 9474
FoxO1: 2308"
Figure legend: "(a) Stable H1299 cells with or without Atg7 knockdown were transfected with a control plasmid (group 1), Flag-FoxO1(ΔDB) (group 2) or Flag-FoxO1(ΔDB-3A) (group 3); 107 cells were then injected into 18 nude mice, with 6 mice per group. Seven weeks later the mice were killed and the tumours were weighed and analysed (bottom graph). Group 1: tumour weights were 1.22 ± 0.78 g (without Atg7 RNAi) and 1.31 ± 0.43 g (with Atg7 RNAi), P = 0.426. Group 2: tumour weights were 0.49 ± 0.18 g (without Atg7 RNAi) and 0.80 ± 0.20 g (with Atg7 RNAi), P = 0.017. Group 3: tumour weights were 1.00 ± 0.40 g (without Atg7 RNAi) and 1.10 ± 0.36 g (with Atg7 RNAi), P = 0.500. Data are means ± s.d. (n = 6)."
NCBI gene IDs:
"Atg7: 10533
FoxO1: 2308"
Figure legend: "(c) HCT116 cells (107) or stable FoxO1-knockdown HCT116 cells (107) were injected into the left or right hind legs of three nude mice. Six weeks later the mice were killed and the tumours were weighed and analysed (bottom graph). Tumour weights were 0.08 ± 0.10 g (without FoxO1 RNAi) and 0.48 ± 0.06 g (with FoxO1 RNAi), P = 0.020. Data are means ± sd. n = 3."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(A) Gross morphology of control (Ctrl), Sas-4−/−, Sas-4−/− 53bp1−/−, and Sas-4−/− Usp28−/− embryos at E9.5. At least five embryos were considered per genotype and all the double mutant embryos exhibited the rescue criteria mentioned in the text. Scale bar = 500 μm."
NCBI gene IDs:
"Sas-4: 219103
53bp1: 27223
Usp28: 57646"
Figure legend: "(B, C) Immunostaining for p53 (B) and Cleaved-Caspase3 (Cl-CASP3, C) on transverse sections of Ctrl, Sas-4−/−, Sas-4−/− 53bp1−/−, and Sas-4−/− Usp28−/− embryos at E9.5. The area shown encompasses the ventral neural tube and surrounding mesenchyme. Asterisks in (B) denote non-specific staining of blood cells. Scale bar = 50 μm. (D, E) Quantifications of the p53 (D) and Cl-CASP3 (E) fluorescence signals shown in (B) and (C), respectively. Three embryos per genotype were used for the quantifications. *** p &lt; 0.001, *p&lt;0.05 (two-tailed student's T-test, one-way ANOVA with Tukey's multiple comparisons tests gave similar results). Bars represent mean ± s.d. (D) Ctrl: 1.00 ± 0.06 (n=1770 cells); Sas-4−/−: 6.84 ± 0.34 (n=275), Sas-4−/− 53bp1−/−: 1.85 ± 0.75 (n=690), Sas-4−/− Usp28−/−: 1.50 ± 0.48 (n=975). (E) Ctrl: 1.00 ± 0.70 (n=1690); Sas-4−/−: 7.88 ± 2.62 (n=175); Sas-4−/− 53bp1−/−: 1.43 ± 0.71 (n=1030); Sas-4−/− Usp28−/−: 1.57 ± 0.44 (n=950). "
NCBI gene IDs:
"Sas-4: 219103
53bp1: 27223
Usp28: 235323"
Figure legend: "(A, B) Whole-mount immunostaining and quantification for p53 on Ctrl and Sas-4−/− embryos at E7.5 (A) and E6.5 (B). Representative sagittal planes are shown and the dotted lines demarcate the epiblast. Quantification of nuclear p53 fluorescence intensity in the epiblast is shown below, normalized to control embryos in the same batch at E7.5 (4 embryos) and at E6.5 (9 embryos). *** p &lt; 0.001 (two-tailed student's T-test). Bars represent mean ± s.d. Scale bars = 100 μm. (A) Ctrl: 1.04 ± 0.00 (n=495 cells); Sas-4-/-: 1.65 ± 0.19 (n=850). (B) Ctrl: 1.00 ± 0.18 (n=1665); Sas-4-/-: 1.16 ± 0.44 (n=1330)"
NCBI gene IDs:
"Sas-4: 219103"
Figure legend: "(A) Immunostaining for the centrosome marker γ-tubulin (TUBG) on Ctrl and Sas-4−/− primary mESCs. The bottom panels are magnifications of the areas marked in the top panels. Scale bars = 20 μm (top) and 10 μm (bottom)."
NCBI gene IDs:
"Sas-4: 219103"
Figure legend: "(B) Immunostaining and quantification for p53 on Ctrl and Sas-4−/− primary mESCs in pluripotent and partially differentiated (diff.) conditions. The quantification of p53 fluorescence intensities was normalized to Ctrl (Four independent experiments). ** p &lt; 0.01, * p &lt; 0.05 (two-tailed student's T-test, the comparison of the genotypes in the &quot;Pluripotent&quot; condition was not significant using the one-way ANOVA with Tukey's multiple comparisons tests). Bars represent mean ± s.d. Scale bar = 50 μm. Pluripotent Ctrl: 1.00 ± 0.02 (n=2030 cells); pluripotent Sas-4-/-: 1.17 ± 0.10 (n=2170); partially diff. Ctrl: 0.62 ± 0.13 (n=2745); partially diff. Sas-4-/-: 1.12 ± 0.24 (n=2000)."
NCBI gene IDs:
"Sas-4: 219103"
Figure legend: "(C, D) Three-day growth curves of WT and Sas-4−/− (C) or p53−/− and Sas-4−/− p53−/− (D) primary mESCs in the indicated conditions starting with 105 cells on Day 0 (Four independent experiments). ** p &lt; 0.01, * p &lt; 0.05 (two-tailed student's T-test). Bars represent mean ± s.d. For details of the measurements, see Materials and Methods (Tables 3 and 4)."
NCBI gene IDs:
"Sas-4: 219103
p53: 22059"
Figure legend: "(E) Northern blot  analysis of circBtnl1  in different tissues of two-month-old mice, 18S RNA  served as a loading control."
NCBI gene IDs:
"18S RNA: 
Btnl1: 100038862"
Figure legend: "(F， G)  RNA fluorescence in situ hybridization   of circBtnl1 in small intestine tissues (F) and organoid (G). Green region shows the distribution of circBtnl1  using antisense probe, gray region shows the nuclei staining  by DAPI. For (F)，scale bars，5um; for (G)，scale bars，20 um."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(C) Representative images of Ki67 staining  of in  small intestinal  tissues from circBtnl1+/+ and circBtnl1-/- mice. For all representative images, at least three independent experiments were performed with similar results. For every microscope field, average Ki67+ ISC  numbers per crypt  are shown as means ± SD. **P &lt; 0.01 by two-tailed Student's t-test in right panel. Scale bar, 50 μm."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(G) Confocal microscopy  of ISCs  makers Olmf4  in organoids from circBtnl1+/+ and circBtnl1-/- mice. Scale bars, 30 μm. Three independent experiments were performed with similar results, and representative experiments are shown. For every microscope field, average Olmf4+ ISC  numbers per crypt  are shown as means ± SD. **P &lt; 0.01 by two-tailed Student's t-test in right panel in right panel."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(B, C) CircBtnl1 was co-localized with Ddx3y  protein in small intestine tissue (B) and organoid  (C) by RNA FISH, followed by immunofluorescence staining. Scale bars, 5 μm."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(G)  Electrophoretic mobility shift assay (EMSA) using biotin-labeled circBtnl1 and recombinant Ddx3y."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(H) Truncated fragments of biotinylated circBtnl1  were incubated with small intestine crypts lysates, followed by RNA pulldown assay  and  Western blot."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(B)  Small intestine crypt  cells were isolated from circBtnl1+/+ and circBtnl1-/- two-month-old mice, followed by RNA extraction and  q-PCR  analysis for top ten upregulated transcription factors. n = 3 independent samples. Results of relative fold changes are shown as means ± SD. **P &lt; 0.01 by two-tailed Student's t-test."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(D) Immunoblotting  analysis of Ddx3y  in RNA pull-down  samples by Atf4  sense, antisense and control probes in mice ISCs. (E) Immunoblotting  analysis of Ddx3y  in RNA  pull-down samples by Atf4  and control probes in circBtnl1+/+ and circBtnl1-/- mice ISCs."
NCBI gene IDs:
"Atf4: 11911
Btnl1: 100038862"
Figure legend: "(J) Confocal microscopy  of ATF4  in Ddx3y  knockdown and overexpression organoids from circBtnl1+/+ and circBtnl1-/- mice. Scale bars, 20 μm."
NCBI gene IDs:
"Btnl1: 100038862
Ddx3y: 26900"
Figure legend: "(K, L) Expression levels of Ddx3y  and Atf4  in circBtnl1+/+ and circBtnl1-/- ISCs  were examined by  real-time PCR (K) and Western blot  (L), three independent experiments were performed. Results of relative fold changes are shown as means ± SD. ** P &lt; 0.01, NS, not significant, by two-tailed Student's t-test."
NCBI gene IDs:
"Atf4: 11911
Btnl1: 100038862
Ddx3y: 26900"
Figure legend: "(D, E) ChIP  assay was performed using anti-H3K4me3 (D) or anti-H3K27me3 (E) with circBtnl1+/+ and circBtnl1-/- ISCs  lysates. **P &lt; 0.01 by two-tailed Student's t-test. Data are representative of at least three independent experiments."
NCBI gene IDs:
"Btnl1: 100038862"
Figure legend: "(H) Confocal microscopy of Sox9  in Atf4  knockdown (Atf4  KD) organoids from circBtnl1+/+ and circBtnl1-/- mice. Scale bars, 30 μm."
NCBI gene IDs:
"Atf4: 11911
Btnl1: 100038862"
Figure legend: "(F) Representative organoid  images of circBtnl1+/+; sgSox9 and circBtnl1-/-; sgSox9 mice, sgCtrl served as control. Organoid  formation  ratios (n = 3) are shown. Organoid  formation  ratios are shown as mean ± s. d. *P &lt; 0.05 by two-tailed Student's t-test. Scale bars, 200 μm."
NCBI gene IDs:
"Btnl1: 100038862
Sox9: 20682"
Figure legend: "okp1-R164C suppressed the temperature-sensitive growth defect of cbf1∆ cse4-R37A. Serial dilutions of strains with the indicated genotypes were spotted on full medium and grown for three days at 30ºC or 37ºC. The original okp1-R164C isolate from the suppressor screen is indicated as \"UV mutagenesis\"."
NCBI gene IDs:
"cbf1: 853523
cse4: 853817
okp1: 853090"
Figure legend: "Serial dilutions of strains with the indicated genotypes were spotted on full medium and grown for three days at 30ºC or 37ºC. The original okp1-R164C isolate from the suppressor screen is indicated as \"UV mutagenesis\". okp1-R164C suppressed the growth defect of cse4-R37A with chl4∆."
NCBI gene IDs:
"chl4: 851841
cse4: 853817
okp1: 853090"
Figure legend: "okp1-R164C was unable to suppress the lethality of cse4-R37A with ame1-4. Tetrad dissection of a genetic cross between a cse4-R37A okp1-R164C and an ame1-4 strain is shown. The four spores from individual asci are aligned in vertical rows."
NCBI gene IDs:
"ame1: 852512
cse4: 853817
okp1: 853090"
Figure legend: "okp1-R164C suppressed the maintenance defect of cse4-R37A for plasmids lacking the CDEI sequence of CEN6 (CEN ∆CDEI, at 37ºC). Error bars give SD of at least three independent transformants. * p = 0.03."
NCBI gene IDs:
"cse4: 853817
okp1: 853090"
Figure legend: "The okp1 mutations I45T, S94T and E208V suppressed the temperature-sensitive growth defect of cbf1∆ cse4-R37A. Strains with the indicated okp1 alleles on a plasmid (derivatives of AEY5584)"
NCBI gene IDs:
"cbf1: 853523
cse4: 853817
okp1: 853090"
Figure legend: "okp1-R164C restored binding of Okp1 to centromeric sequences. ChIP of 9xmyc-tagged Okp1 was performed in strains with the indicated genotypes that were grown at 37ºC for 4 hours before ChIP. Enrichment of CEN4 and POL1 (as a control) relative to input is given. Below, Western blot analysis of the amounts of 9xmyc-Okp1 and histone H2B (loading control) in whole cell extracts."
NCBI gene IDs:
"POL1: 
okp1: 853090"
Figure legend: "okp1-R164C restored binding of Ame1 to centromeric sequences. ChIP of 9xmyc-tagged Ame1 was performed as in (A). Below, Western blot analysis of the amounts of 9xmyc-Ame1 and H2B as in (A)."
NCBI gene IDs:
"okp1: 853090"
Figure legend: "okp1-R164C restored binding of Mtw1 to centromeric sequences. ChIP of 9xmyc-Mtw1 and Western blots (right)"
NCBI gene IDs:
"okp1: 853090"
Figure legend: "okp1-R164C improved in vivo association of Okp1 with Cse4-R37A. Co-immunoprecipitation of Cse4-R37A and Okp1 was carried out in cells carrying cse4-R37A with (+) or without (-) 3xHA-tag and OKP1 or okp1-R164C with (+) or without (-) 9xmyc-tag (AEY5040, AEY5972, AEY5973, AEY6589, AEY6592). Cells were grown at 23ºC or were shifted to 37ºC for 5 hours prior to harvesting. Cse4-R37A was immunoprecipitated using an α-HA antibody, and the presence of Cse4-R37A and Okp1 or Okp1-R164C in the immunoprecipitate was tested by Western blotting with α-HA and α-myc antibody, respectively. Left, inputs (the two bands indicate Okp1 and a shorter degradation product); right, α-HA immunoprecipitates."
NCBI gene IDs:
"HA: 
myc: 
cse4: 853817
OKP1: 853090
okp1: 853090"
Figure legend: "The ame1 mutations S59P, N159H, S275N and a C-terminal truncation after aa 273 (ame1-273*) suppressed the growth defect of cbf1∆ cse4-R37A. cbf1∆ cse4-R37A ame1∆ strains carrying the indicated plasmid-borne ame1 alleles"
NCBI gene IDs:
"ame1: 852512
cbf1: 853523
cse4: 853817"
Figure legend: "Mimicking the unacetylated state of Cse4-K49 (cse4-K49R) partially suppressed the temperature-sensitivity of cbf1∆ cse4-R37A. cbf1∆ cse4∆ strains carrying the indicated cse4 alleles on a plasmid were spotted on full medium and grown"
NCBI gene IDs:
"cbf1: 853523
cse4: 853817
Cse4: 853817"
Figure legend: "cse4-K49R suppressed the lethality of cse4-R37A with ctf19∆. Tetrad dissection of a ctf19∆ strain crossed with cse4-R37A (left) and cse4-R37A K49R (right). Representation"
NCBI gene IDs:
"cse4: 853817
ctf19: 856089"
Figure legend: "Acetylation of Cse4-K49 was reduced in the absence of Gcn5 and the SAGA complex, but not in the absence of the HAT Sas2. Cse4 was precipitated as in (A) from strains wild-type (wt) or gcn5∆ (top) or sas2∆ strains (bottom) carrying HA-tagged Cse4. w/o, control immunoprecipitation without antibody."
NCBI gene IDs:
"HA: 
Cse4: 853817
Gcn5: 853167
gcn5: 853167
Sas2: 855157
sas2: 855157"
Figure legend: "Cse4-K49Ac depended on the SAGA components Ada2 and Spt7. Cse4-K49Ac was determined as in (A, left) with cells carrying ada2∆ or spt7∆."
NCBI gene IDs:
"Ada2: 852059
ada2: 852059
spt7: 852373
Spt7: 852373"
Figure legend: " WT and hCDC14APD RPE1 cells were serum starved for 48 h for inducing ciliogenesis prior to fixation. Cilia were stained with Arl13B (green) while the basal bodies were marked with C-Nap1 (red). DNA was stained with DAPI. Cilia in the magenta box are shown enlarged on the right picture "
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " WT and hCDC14APD RPE1 cells were serum starved for 48 h for inducing ciliogenesis prior to fixation. Cilia were stained with Arl13B (green) while the basal bodies were marked with C-Nap1 (red). DNA was stained with DAPI Percent of ciliated cells, as well as the length of cilia, was quantified from A. Cilia length was measured by a semi-automated ImageJ macro as described in methods. Data points are the average of three independent experiments with N = 150 cilia for each experiment and condition. Error bars represent the mean ± SD. **** P≤ 0.0001. High means 80-90% and low 40-50% confluency of RPE1 cells"
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " Elongated cilia phenotype was confirmed by siRNA-mediated knockdown of hCDC14A. Three independent experiments, N = 150 cilia for each experiment and condition. Mean ± SD. **** P≤ 0.0001 "
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " hCDC14A-GFP and hCDC14AC278S-GFP under control of the TetON promoter were stably integrated into RPE1 cells. hCDC14A-GFP and hCDC14AC278S-GFP localized to the C-Nap1 marked basal body upon Dox induction. In addition, expression of hCDC14A-GFP but not the phosphatase dead hCDC14AC278S-GFP mutant inhibited cilia formation. Ciliated cells were quantified 48 h after serum starvation with and without Dox addition. Three independent experiments; N = 150 cilia for each experiment and condition. Mean ± SD. *** P≤ 0.001; ns: not significant "
NCBI gene IDs:
"GFP: 
hCDC14A: 8556"
Figure legend: " RPE1 cells over-expressing hCDC14AC278S have longer cilia than those over-expressing hCDC14A. Three independent experiments, N = 100 cilia for hCDC14A expressing cells and 200 for hCDC14AC278S expressing cells. Mean ± SD. *** P≤ 0.001 "
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " The dynamics of ciliogenesis was determined by serum starving WT and hCDC14APD cells for different time-points. The cilia from hCDC14APD cells continue to elongate even after 48 h of starvation whereas those from WT cells reached constant length within 12 to 24 h. Three independent experiments; N = 150 cilia for each experiment and condition. Mean ± SEM. * P≤ 0.05, **; P≤ 0.01; **** P≤ 0.0001"
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " In a time course experiment, the cilia disassembly kinetics was measured by first inducing ciliation through 48 h of serum starvation prior to incubation with serum containing media for different time points. Measurement of cilia length indicated the comparable rates of cilia disassembly in RPE1 WT and hCDC14APD cells. Cilia disassembly rates are shown in Fig. EV1D. Three independent experiments, N = 150 cilia for each experiment and condition. Mean ± SEM. **** P&lt;0.0001 "
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: " Images of cilia of WT RPE1 and hCDC14APD RPE1 cells from I, 0, 6 and 24 h after serum addition. Fixed cells were analyzed for cilia length "
NCBI gene IDs:
"hCDC14A: 8556"
Figure legend: "TetON-hCDC14A-GFP RPE1 cells were serum starved for 48 h prior to fixation and immunofluorescence microscopy. hCDC14A-GFP colocalizes with Phalloidin 555 conjugated dye that marks F-actin fibers. The boxed area was 10-fold enlarged and displayed below the corresponding images to indicate co-localization of hCDC14A-GFP and F-actin. Line scan inside the magenta colored box showed in (A)"
NCBI gene IDs:
"GFP: 
hCDC14A: 8556"
Figure legend: "hCDC14A, not the inactive hCDC14AC278S, dephosphorylates the serine residue 142 of DBN1 in vivo. RPE1 cells expressing GFP, hCDC14A-GFP, and hCDC14AC278S-GFP under TetON promoter were serum starved in presence or absence of Dox for 48 h prior to fixation for immunofluorescence. DBN1pS142 was detected with a phospho-specific antibody. DNA was stained with DAPI. Scale Bar = 10 μm"
NCBI gene IDs:
"GFP: 
hCDC14A: 8556"
Figure legend: "DBN1S142 was hyper-phosphorylated in hCDC14APD cell lines during ciliogenesis compared to the WT control. In this experiment we sequentially analyzed DBN1pS142 and DBN1 on the same blot. The specificity of the DBN1pS142 antibody was validated by siRNA mediated knock-down of DBN1 in both hCDC14APD cells and GFP/hCDC14A-GFP/hCDC14AC278S-GFP stable cell lines. Three independent experiments. Mean ± SD. ** P≤ 0.01, *** P≤ 0.001"
NCBI gene IDs:
"GFP: 
hCDC14A: 8556
DBN1: 1627"
Figure legend: "In vitro dephosphorylation of DBN1 by purified hCDC14A. DBN1-GFP was transfected into HEK293T cells and expressed for 48 h. DBN1-GFP was immunoprecipitated from cell lysates with GFP binder. The immunoprecipitated phospho-DBN1 was then incubated with and without recombinant hCDC14A in vitro. The DBN1pS142/DBN1 ratio was densitometrically measured. Three independent experiments. Mean ± SD. **** P≤ 0.0001"
NCBI gene IDs:
"DBN1: 
GFP: "
Figure legend: "One targeted clone from each gRNA (1.16, 2.18, 3.3) including the non-targeted clone 2.19 and WT control were serum starved for 48 h to induce ciliogenesis. Subsequently, cilia length was measured. The cilia from DBN1 KO cells were significantly longer than those from WT or non-targeted controls. Three independent experiments; N = 150 cilia for each experiment and condition. Mean ± SD. **** P≤ 0.0001."
NCBI gene IDs:
"DBN1: 1627"