EMBO/SourceData huggingface dataset:
Figure legend: " (C) IL-6 released from primary MoMacs (n=5) treated with non-targeting (NT), TLR2- or MyD88-specific siRNA relative to NT condition one-sample t-tes"
NCBI gene IDs:
"MyD88: 4615
TLR2: 7097"
Figure legend: " (E) IL-6 protein released from mock or TLR2 Cas9-CRISPR-edited THP-1 cells (n=3) I one representative of 'n' independent experiments is shown (mean+SD) ), Student's t-tes"
NCBI gene IDs:
"TLR2: 7097"
Figure legend: " (F) NF-κB activity in empty vector (EV) or TLR2-transfected HEK293T cells (n=3) I one representative of 'n' independent experiments is shown (mean+SD) "
NCBI gene IDs:
"TLR2: 7097"
Figure legend: " (G) MPO release from intradermally injected WT or Tlr2 KO mice (n=5-15 biopsies each) Data information I represent data (mean+SEM) combined from 'n' technical or biological replicate mic ), Student's t-tes"
NCBI gene IDs:
"Tlr2: 24088"
Figure legend: " (H) BALF IL-6 and TNF from intratracheally treated WT or Tlr2 KO mice (n=4-5 mice each) one-way ANOVA with Dunnett's multiple compariso "
NCBI gene IDs:
"Tlr2: 24088"
Figure legend: " (I) Relative IL10 mRNA in whole blood of WT or heterozygous TLR2 R753Q carriers (n=5 each) Data information: I represent data (mean+SEM) combined from 'n' technical or biological replicate (human donor * p&lt;0.05 according to Mann-Whitney"
NCBI gene IDs:
"IL10: 3586
TLR2: 7097"
Figure legend: " (A) NF-κB activity in TLR2-transfected HEK293T cells (n=3) Data information I one representative of 'n' independent experiments is shown (mean+SD) Student's t-tes"
NCBI gene IDs:
"TLR2: 7097"
Figure legend: " (G) Relative cytokine mRNAs in whole blood stimulation (n=5-13) Data information represent data (mean+SEM) from 'n' technical or biological replicates (donors) * p&lt;0.05 according to Wilcoxon signed rank su"
NCBI gene IDs:
"cytokine: 3586"
Figure legend: "(H) NF-κB activation in TLR2-transfected HEK293T cells (n=3 Data information I one representative of 'n' independent experiments is shown (for asmean+SD) ), Student's t-tes"
NCBI gene IDs:
"TLR2: 7097"
Figure legend: "(B) ZNF366 expression across clusters."
NCBI gene IDs:
"ZNF366: 167465"
Figure legend: "MAFF expression was silenced using a lentivirus containing shRNA. (H) Protein quantification by Western Blot after 5 days. Actin was used as loading control. Representative results are shown (n=4 biological replicates). Quantification was performed by densitometry. Each symbol represents an individual donor. Paired one-way Anova."
NCBI gene IDs:
"MAFF: 23764"
Figure legend: "MAFF expression was silenced using a lentivirus containing shRNA. (I) Mo-mac and mo-DC differentiation after 5 days was assessed by flow cytometry. One representative donor is shown (n=7 biological replicates). DN = double negative. Proportion of mo-DC, mo-Mac and DN cells after 5 days. Median is shown (n=7 biological replicates). Paired one-way Anova."
NCBI gene IDs:
"MAFF: 23764"
Figure legend: "YTHDC1 accumulation within nSBs was arrested by METTL3 knockdown. HeLa cells were stained as described in Figure 1C. Scale bar: 10 µm. Box plot of relative intensities of YTHDC1 to HSATIII within nSBs in individual nuclei (n=30). The mean is indicated with X. The first and third quartiles are the ends of the box, the median is indicated with a vertical line in the box, and the minimum and maximum except for the outliers are the ends of the whiskers. The outliers are indicated with open circles. P-values (Kruskal-Wallis test, followed by Dunn's multiple comparison test) are shown above."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "Scatter plot of LogFC of whole introns (grey) and HSATIII-target introns (blue) upon SRSF9 KD (X-axis) and HSATIII KD (Y-axis). 51,469 and 548 introns are plotted as whole introns and previously-reported HSATIII-target introns, respectively. Pearson's correlation (r) is indicated above."
NCBI gene IDs:
"SRSF9: 8683"
Figure legend: "Read maps represent RNA-seq data of nuclear poly (A)+ RNAs (upper panel, biological triplicate) and m6A-RIP RNAs (lower panel, biological duplicate) in control and HSATIII KD cells. The exon numbers of the FAM214A gene are indicated below. The retaining intron and the m6A peak are marked by a red line and arrow, respectively. For the entire FAM214A region, see also Figure EV4B."
NCBI gene IDs:
"FAM214A: 56204"
Figure legend: "(A) Wild type (left and middle panels) and p53-/- (right panel) HCT116 cells were transfected with control (mixture of control DS scrambledNeg, siLuci, and siGFP) or GRWD1-targeting (mixture of siGRWD1-3 and 4) siRNAs for 36 h, treated with 5 nM actinomycin D, and harvested at the indicated times. Whole cell extracts were analyzed by immunoblotting with the indicated antibodies. Coomassie Brilliant Blue (CBB) staining serves as a loading control."
NCBI gene IDs:
"GRWD1: 83743
p53: 7157"
Figure legend: "(B) Control U2OS cells infected with control retroviruses with the empty vector and U2OS cells overexpressing the siRNA-resistant HA-GRWD1 were treated and analyzed as above."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(C) HCT116 cells were transfected with control (mixture of control DS scrambledNeg, siLuci, and siGFP) or GRWD1-targeting (mixture of siGRWD1-3 and 4) siRNAs for 24 h, treated with 50 µg/ml cycloheximide, and harvested at the indicated times. Whole cell extracts were analyzed by immunoblotting with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(D) HFF2/T cells stably overexpressing HA-GRWD1 were established by retroviral infection. Cells were cultured in the presence or absence of actinomycin D (5 nM) for 12h and then treated with or without MG132 (20uM) for 6 h as indicated. Whole cell extracts were analyzed as above."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(E) Control and HA-GRWD1-overexpressing HFF2/T cells were first treated with actinomycin D (5nM) for 12 h, then further treated with 50 ug/ml cycloheximide, and harvested at the indicated times. Whole cell extracts were analyzed by immunoblotting with the indicated antibodies. The means and SDs from two independent experiments are shown."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(A) U2OS cells were transfected with control (mixture of control DS scrambledNeg, siLuci, and siGFP) or GRWD1-targeting (mixture of siGRWD1-3 and 4 or siGRWD1-1) siRNAs for 24 h. Whole cell extracts were then analyzed by immunoblotting with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(D) HCT116 cells were transfected with control (mixture of control siLuci and siGFP) or GRWD1-targeting (mixture of siGRWD1-3 and 4 or siGRWD1-1) siRNAs for 24 h and then immunostained as above. Scale bar, 20 um."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(A) 293T cells were co-transfected with HA-GRWD1 (1.6 ug) and RPL5-FLAG (2.4 ug) or RPL11-FLAG (2.4 ug) as indicated for 42h and subjected to immunoprecipitation with anti-FLAG antibody. Immunoprecipitates (IPs) and 3.125% of inputs were immunoblotted with the indicated antibodies. *, immunoglobulin light chains."
NCBI gene IDs:
"RPL11: 6135
RPL5: 6125"
Figure legend: "(E) Cell lysates were prepared from 293T cells transfected with wild-type (WT) HA-GRWD1-FLAG (12 µg) or various N-terminal truncated mutants of HA-GRWD1-FLAG (12 µg) for 42h, and then subjected to GST pull-down assay with purified GST-RPL11 or GST. Bound proteins were analyzed by CBB staining (GST and GSR-RPL11) and immunoblotting with the indicated antibodies. *, non-specific bands."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(G) HCT116 cells were transfected with RPL11-FLAG expression vector (0.56 ug) or empty vector (0.56 ug) for 24 h and then treated with 5 nM actinomycinD or vehicle (PBS) for 12 h. The cells were first extracted with Triton X-100 to remove nucleoplasmic proteins, double-immunostained with anti-GRWD1 antibody (green) and anti-FLAG M2 antibody (red), and counterstained with DAPI. Scale bar, 20 um."
NCBI gene IDs:
"RPL11: 6135"
Figure legend: "(A) H1299 cells were co-transfected with the indicated expression vectors (p53, 7.5 ng; HA-Ub, 0.5 ug; His-Xpress-MDM2, 2 ug; RPL11-FLAG, 1 ug; HA-GRWD1-FLAG, 1.5 ug; GFP, 0.04 ug) for 48 h and analyzed by immunoblotting with the indicated antibodies. GFP serves as a control protein to show equal transfection efficiencies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135
p53: 7157
Ub: 7314///7311///6233///7316"
Figure legend: "(B) Lysates were prepared from 293T cells co-transfected with His-Xpress-MDM2 (1.2 ug), FLAG-RPL11 (1.8 ug), and HA-GRWD1 (1 ug) as indicated for 42 h and then immunoprecipitated with anti-FLAG antibody. Immunoprecipitates (IPs) and 1.5% of inputs were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135"
Figure legend: "(C) In vivo ubiquitination assay to detect MDM2 autoubiquitination in H1299 cells. Lysates were prepared from H1299 cells transfected with the indicated expression vectors (His-Xpress-MDM2, 2 ug; HA-Ub, 0.5 ug; RPL11-FLAG, 1 ug; HA-GRWD1-FLAG, 1.5 ug) for 48 h, treated with proteasome inhibitors for 6 h before harvest, and then immunoprecipitated with anti-MDM2 antibody. Immunoprecipitates (IPs) and inputs were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135
Ub: 7314///7311///6233///7316"
Figure legend: "(D) In vitro ubiquitination of p53 by immunopurified MDM2. His-Xpress-MDM2 was immunopurified from transfected 293T cells with anti-Omni probe antibody. Recombinant p53 was incubated with E1, E2 (UbcH5a), His-ubiquitin, ATP, GST-RPL11, GRWD1-His, and immunopurified His-Xpress-MDM2 or control immunoprecipitates at 30℃ for 120 min as indicated. The samples were resolved by SDS-PAGE followed by immunoblotting with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135"
Figure legend: "(A) Expression of the introduced proteins and the effect on p53. HFF2/T cells stably expressing HA-GRWD1, E7, and KRAS G12V were established with retroviral vectors and analyzed by immunoblotting with the indicated antibodies. *, endogenous RAS proteins."
NCBI gene IDs:
"E7: 1489079
GRWD1: 83743
KRAS: 3845"
Figure legend: "(B and C) Anchorage-independent growth of HFF2/T cells overexpressing E7 with or without HA-GRWD1. Three weeks after seeding, images were acquired and colonies &gt;100 µm in diameter were counted. Representative images are shown in (B). Scale bars, 500 um. Average numbers of colonies per field, with SDs, are shown in (C). Six randomly selected areas were counted per sample."
NCBI gene IDs:
"E7: 1489079
GRWD1: 83743"
Figure legend: "(D and E) Anchorage-independent growth of HFF2/T cells overexpressing E7 and activated KRAS G12V with or without HA-GRWD1. At 9 days after seeding, the colonies were analyzed as above. Representative images are shown in (D). Scale bars, 500 um. The average numbers of colonies (&gt;200 um in diameter) per field, with SDs, are shown in (E). Twenty randomly selected areas were counted."
NCBI gene IDs:
"E7: 1489079
GRWD1: 83743
KRAS: 3845"
Figure legend: "(F) Tumorigenesis of HFF2/T cells expressing E7 and KRAS G12V with or without HA-GRWD1. Cells were subcutaneously injected into nude mice (1 x 106 cells/mouse), and tumor size was measured. Average tumor volumes, with SDs, are shown (n=5)."
NCBI gene IDs:
"E7: 1489079
GRWD1: 83743
KRAS: 3845"
Figure legend: "(G) High expression of GRWD1 is associated with poor prognosis of brain lower grade glioma patients with wild type p53 but not with mutated p53. The data were obtained from the TCGA databases (the cBioPortal for Cancer Genomics; http://www.cbioportal.org). For detail, see Materials and Methods."
NCBI gene IDs:
"GRWD1: 83743
p53: 7157"
Figure legend: "(A) 293T cells were co-transfected with HA-GRWD1 wild-type (WT) (0.5 ug) or HA-GRWD1 Δ1-136 (3.5 ug) and RPL11-FLAG (1.5 ug) as indicated for 42h, and then immunoprecipitated with an anti-FLAG antibody. Immunoprecipitates (IPs) and 1.6% of inputs were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743
RPL11: 6135"
Figure legend: "(B) 293T cells were co-transfected with His-Xpress-MDM2 (0.6 ug), RPL11-FLAG (0.9 ug), and HA-GRWD1 WT (0.5 ug) or HA-GRWD1 Δ1-136 (4.5 ug) as indicated for 42h and subjected to immunoprecipitation with an anti-FLAG antibody. Immunoprecipitates (IPs) and 1.6% of inputs were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135"
Figure legend: "(C) H1299 cells were co-transfected with the indicated expression vectors (p53, 7.5 ng; HA-Ub, 0.5 ug; His-Xpress-MDM2, 2 ug; RPL11-FLAG, 1 ug; HA-GRWD1-FLAG WT, 1.5 ug; HA-GRWD1-FLAG Δ1-136, 1.5 ug; GFP, 0.04 ug) for 48 h, and then analyzed by immunoblotting with the indicated antibodies. GFP serves as a control protein to show equal transfection efficiencies."
NCBI gene IDs:
"GRWD1: 83743
MDM2: 4193
RPL11: 6135
p53: 7157
Ub: 7311///6233///7316///7314"
Figure legend: "(D) HFF2/T cells stably expressing HA-GRWD1 wild-type (WT) or Δ1-136, E7, and KRAS G12V were established using retroviral vectors and analyzed by immunoblotting with the indicated antibodies. *, endogenous RAS proteins."
NCBI gene IDs:
"GRWD1: 83743"
Figure legend: "(E and F) Anchorage-independent growth of HFF2/T cells overexpressing E7 and activated KRAS G12V with HA-GRWD1 WT or Δ1-136 (or without HA-GRWD1). At 9 days after seeding, colonies were analyzed as above. Representative images are shown in (E). Scale bars, 500 um. Average numbers of colonies (&gt;200 um in diameter) per photographed area with SDs are shown in (F). Seventy randomly selected areas were counted."
NCBI gene IDs:
"E7: 1489079
GRWD1: 83743
KRAS: 3845"
Figure legend: "C,D,E Examples of embryos expressing the tFT-Bcd reporter with C: mCherry-sfGFP-Bcd, D: fmCherry-sfGFP-Bcd, and E: mCherry-sfGFP. For all, (i) Images of \"shells\" of embryos in early n.c. 14. 3D images of the embryos were generated, and then the interior of the embryo was erased, leaving only the embryo cortex to improve clarity (ii) Mean AP intensity profile of each color for the embryo in i. Shade region represents ±1 s.d. Inset shows same profiles after multiplication of red intensity by constant factor. Data binned into 10 µm bins (n=4 embryos). (iii) Ratio of green over red signal, reflecting protein age. The thin lines represent individual embryos, while the thick solid line is the mean. The solid dashed line depicts the mean green/red ratio for a line with the tFT-Bcd reporter but lacking endogenous Bcd (BcdE1 mutant, n=4)."
NCBI gene IDs:
"Bcd: 40830"
Figure legend: "D The age of Bcd and its gradient are unaltered in fsd mutant. Comparison of a representative y/w embryo (left) and loss-of-function mutation fsdKG02393 (right) are shown. Both embryos have the tFT-Bcd construct in the same locus, are in early n.c. 14, were imaged side-by-side under identical conditions, and are displayed with the same settings. Embryo \"shells\" are displayed."
NCBI gene IDs:
"fsd: 36463"
Figure legend: "E Quantification of embryos Fluorescence intensity from sfGFP (green) and mCherry (magenta) for wt (N=8, filled circles) and fsd (N=6, open squares) at early cycle 14. Inset: tFT-Bcd ratio for wt (black) and FSD (gray) embryos."
NCBI gene IDs:
"fsd: 36463
FSD: 36463"
Figure legend: "G, Fluorescent in situ hybridization for cd74a, tnfa, and ctsc at 7 dpi, respectively. mpeg1 was used to label all MC cells. The blue boxed region is highlighted in the zoom-in image to the right. Scale bar = 50 μm. A stands for arium, V stand for ventricle and OFT stands for outflow tract. White dashed lines outline the heart and the yellow dashed lines indicate approximate resection plane."
NCBI gene IDs:
"cd74a: 58113
ctsc: 368704
mpeg1: 335407
tnfa: 405785"
Figure legend: "F, In situ hybridization showing temporal spatial expression patterns of postnb. The white boxed region is shown in zoom-in images at the bottom. Scale bar = 25 μm. White dashed lines outline the heart and the yellow dashed lines indicate approximate resection plane. A stands for arium, V stand for ventricle and OFT stands for outflow tract."
NCBI gene IDs:
"postnb: 337176"
Figure legend: "H, Fluorescent in situ hybridization for postnb, tnfα, and tnfrsf1a in the injury area at 7 dpf, respectively. The white boxed regions are shown in their respective zoom-in images to the right. White dashed lines outline cardiac apex and the yellow dashed lines indicate approximate resection plane. Scale bar = 25 μm."
NCBI gene IDs:
"postnb: 337176
tnfα: 405785
tnfrsf1a: 406471"
Figure legend: "J, Violin plots showing expression of cxcl18b, sele, atf3, and fosl1a in each eEC subpopulation."
NCBI gene IDs:
"atf3: 393939
cxcl18b: 795785
fosl1a: 564241
sele: 558139"
Figure legend: "K, Fluorescent in situ hybridization for fosl1a at 7 dpi. The white boxed region is shown in a higher magnification image to the right for the injury area. cdh5 was used to label all EC cells and the red boxed region is shown in a higher magnification image to highlight a pan-EC cdh5 expression in green. White dashed lines outline the heart and the yellow dashed lines indicate approximate resection plane. Scale bar = 25 μm. A stands for arium, V stand for ventricle and OFT stands for outflow tract."
NCBI gene IDs:
"cdh5: 445471
fosl1a: 564241"
Figure legend: "A, Wildtype or kit mutant stained with AFOG at 14 days and 30 dpi. Sham-operated zebrafish hearts serve as control. Scale bar = 100 μm."
NCBI gene IDs:
"kit: 30256"
Figure legend: "D, F, Violin plots showing the expression of il1b in MC1 (D), and ctsd expression in MC (F). E, G, Expression of il1b (E) and ctsd (G) in WT and kit mutant hearts determined by qRT-PCR."
NCBI gene IDs:
"ctsd: 65225
il1b: 405770
kit: 30256"
Figure legend: "O, Violin plots showing expression of junba in eEC. P, Expression of junba in WT and kit mutant hearts determined by qRT-PCR."
NCBI gene IDs:
"junba: 407086
kit: 30256"
Figure legend: "C T98G cells were treated with non-targeting (CTRL) or KU70-targeting siRNAs prior to whole cell extract preparation, and pull-down as in B. Inputs and eluates were analyzed by WB using indicated antibodies."
NCBI gene IDs:
"KU70: 2547"
Figure legend: "A T98G cells stably expressing cGAS (T98GcGAS) or not (T98GEmpty) were transfected or not with dsDNA for 6 h prior to whole cell extraction and WB analysis using indicated antibodies."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "C T98GEmpty and T98GcGAS were transfected or not with biotinylated dsDNA prior to whole cell extraction and pull-down using streptavidin-affinity beads. Inputs and eluates were analyzed by WB using indicated antibodies."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "J T98GcGAS were transfected or not with dsDNA in presence or not of NU7441, prior to WB analysis using indicated antibodies."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "F T98GEmpty and T98GcGAS stably expressing a GFP reporter (T98G-GFPEmpty and T98G-GFPcGAS, respectively) were xenotransplanted into the head of tg(mfap4:RFP) zebrafish line at 3 days post fertilization (dpf). Zebrafish embryos were imaged daily over 3 days. The graph represents the mean (±SEM) percentage of tumor growth normalized by the area on the day of transplantation (n=21 T98G-GFPEmpty and n=29 T98G-GFPcGAS embryos)."
NCBI gene IDs:
"GFP: 
RFP: 
cGAS: 115004
mfap4: 405825"
Figure legend: "A Graph represents the mean (± SEM) fold migration of THP-1 cells through a 3 µm transwell insert when conditioned media from T98GEmpty or T98GcGAS was applied to lower chamber for 6 h. CCL2 was used as positive control (n=8 biological replicates)."
NCBI gene IDs:
"cGAS: 115004"
Figure legend: "E Graph presents the quantification of macrophages recruited at tumor site 24 h post xenotransplantation in D (n=21 T98G-GFPEmpty and n=29 T98G-GFPcGAS embryos)."
NCBI gene IDs:
"GFP: 
cGAS: 115004"
Figure legend: "H Violin plots present the expression of PRKDC and MB21D1 in datasets analyzed in A, based on tumor grade (II to IV)."
NCBI gene IDs:
"MB21D1: 115004
PRKDC: 5591"
Figure legend: "F. Relative mRNA expression  of IL-22, IL-17a, IFN-γ, IL-1β, IL-6, IL-10, Muc1  of colon  tumour described in (A) was shown. Data information: All data are mean ± s.e.m. NS, not significant. *P &lt; 0.05, **P &lt; 0.01, ****P &lt; 0.0001 based on two-tailed Student's t test Each dot represents one mouse, n=15. Data are representative of three biological replicates."
NCBI gene IDs:
"IFN-γ: 15978
IL-10: 16153
IL-17a: 16171
IL-1β: 16176
IL-22: 50929
IL-6: 16193
Muc1: 17829"
Figure legend: "representative histological  images (B) of wild-type control mice  or BTNL2-KO mice  treated with 2% DSS  for 8 days were shown (n=15)."
NCBI gene IDs:
"BTNL2: 547431"
Figure legend: "E. Relative mRNA expression  of  IL-22, RegⅢγ, RegⅢβ, Muc1, IL-17a,  IFN-γ, IL-1β, IL-6 and  IL-10 of colonic  tissues described in (A) was shown. Data information: All data are mean ± s.e.m. NS, not significant. **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 based on two-tailed Student's t test Each dot represents one mouse, n=15. Data are representative of three biological replicates."
NCBI gene IDs:
"IFN-γ: 15978
IL-10: 16153
IL-17a: 16171
IL-1β: 16176
IL-22: 50929
IL-6: 16193
Muc1: 17829
RegⅢβ: 18489
RegⅢγ: 19695"
Figure legend: "representative H&amp;E images (H) of wild-type control mice  or BTNL2-KO mice  treated with Fc or mIL-22-Fc (ip. 5 μg/mouse) at day 0, 2, 4, 6 during 2% DSS  treatment were shown (n=12). Data information Data are representative of three biological replicates H)."
NCBI gene IDs:
"BTNL2: 547431"
Figure legend: "G. IL-22, IL-17a, RORC  and HIF-1α expression in splenocytes  after cultured in plate-coated Fc or mBTNL2-Fc (30 μg/mL) for 20 h was analyzed by  real-time PCR. Data information: All data are mean ± s.e.m. NS, not significant., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 based on two-tailed Student's t test Each dot represents one repetition, n=6 Data are representative of three biological replicates"
NCBI gene IDs:
"HIF-1α: 15251
IL-17a: 16171
IL-22: 50929
RORC: 19885"
Figure legend: "E. Relative mRNA expression  of IL-22, RegⅢγ, RegⅢβ, Muc1,  IL-17a,  IFN-γ, IL-1β and IL-6 from colonic  tissues described in (A) was shown. Data information: All data are mean ± s.e.m. NS, not significant., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 based on two-tailed Student's t test Each dot represents one repetition, n=15 Pooled data from three biological replicates"
NCBI gene IDs:
"IFN-γ: 15978
IL-17a: 16171
IL-1β: 16176
IL-22: 50929
IL-6: 16193
Muc1: 17829
RegⅢβ: 18489
RegⅢγ: 19695"
Figure legend: "J. Relative mRNA expression  of IL-22, RegⅢγ, RegⅢβ, Muc1,  IL-17a, IFN-γ,  IL-1β and  IL-6 of colonic  tissue described in (F) was shown. Data information: All data are mean ± s.e.m. NS, not significant., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 based on two-tailed Student's t test Each dot represents one repetition, n=12 Pooled data from three biological replicates"
NCBI gene IDs:
"IFN-γ: 15978
IL-17a: 16171
IL-1β: 16176
IL-22: 50929
IL-6: 16193
Muc1: 17829
RegⅢβ: 18489
RegⅢγ: 19695"
Figure legend: "F. Relative mRNA expression  of IL-22,  IL-17a,  IFN-γ, IL-1β, IL-6, IL-10 and Muc1  of colonic  tumour described in (A) was shown. Data information: All data are mean ± s.e.m. NS, not significant., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 based on two-tailed Student's t test for F, Each dot represents one mouse, n=12. Pooled data from three biological replicates"
NCBI gene IDs:
"IFN-γ: 15978
IL-10: 16153
IL-17a: 16171
IL-1β: 16176
IL-22: 50929
IL-6: 16193
Muc1: 17829"
Figure legend: "(A Representative confocal images of HeLa and TRIM16KO cells treated with (A) MG132 (20 µM, 2h), scale bar: 7.5 µm. and the samples were processed for IF analysis with Ub and p62 antibody. (B Representative confocal images of control siRNA and TRIM16 siRNA transfected cells treated with (B) MG132 (20 µM, 2h), where IF analysis was conducted with Ub and p62 antibodies. (C The graph shows the the percentage of cells with Ub-p62 co-localized dots. Data from ≥10 fields (40X), n=3, Mean ±SD, **p &lt; 0.0003, *p &lt; 0.002 (Student's unpaired t-test). Data information: Unless otherwise stated, scale bar: 10 µm."
NCBI gene IDs:
"TRIM16: 10626"