EMBO/SourceData huggingface dataset:
Figure legend: "(C) MEFs were transfected and treated with CCCP as described in A, followed by an immunoblotting analysis with antibodies for cytochrome c, actin, and parkin. Note that levels of parkin R275W and R42P mutant were lower, as previously reported (Wang et al., 2005)."
NCBI gene IDs:
"parkin: 56816"
Figure legend: "CCCP-induced parkin-mitochondrial aggregate formation is an intermediate step for mitophagy. (A) MEFs expressing WT GFP-parkin were treated with CCCP or CCCP and nocodazole (NOC, 10 µM) for 8, 16, and 24 h as indicated. Cells were immunostained with anti-Tom20 to visualize mitochondria. GFP-parkin-positive cells are marked by dotted lines. Arrows indicate parkin-positive mitochondria or mitochondrial aggregates. Bar, 25 µm. (B) The average percentages of cells with mitochondrial aggregates or without mitochondria from three independent experiments from A are presented with standard deviation as error bar. **, P &lt; 0.01."
NCBI gene IDs:
"parkin: 56816"
Figure legend: "(A) MEFs were transfected with GFP wild-type (WT), A240R, and T415N mutant parkin followed by CCCP treatment for 8 h. Cells were immunostained with antibodies for cytochrome c (red) and ubiquitin (blue). Arrows indicate mitochondrial aggregates. Bar, 10 µm. (B) The average percentages of ubiquitin-positive mitochondrial aggregates from three independent experiments from A are presented with standard deviation as error bar."
NCBI gene IDs:
"parkin: 56816"
Figure legend: "Parkin-mediated ubiquitination recruits p62 and HDAC6. MEFs were transfected with WT, A240R, and T415N mutant GFP-parkin followed by CCCP treatment for 8 h. Cells were double immunostained with cytochrome c (red) and p62 antibody (blue) in A, and cytochrome c (red) and HDAC6 antibody in C. Arrows indicate mitochondrial aggregates. (B and D) The average percentages of p62- or HDAC6-positive mitochondrial aggregates from three independent experiments are presented with standard deviation as error bar."
NCBI gene IDs:
"Parkin: 56816"
Figure legend: "(E) Wild-type and HDAC6 knockout (KO) MEFs were transfected with parkin-GFP or cotransfected with a Flag-tagged HDAC6 followed by CCCP treatment for 16 h as indicated. Cells are immunostained with Tom20 (red) and Flag (blue) antibodies. Bar, 25 µm."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(F) Wild-type MEFs were transfected with control or HDAC6 siRNA and parkin-GFP, and treated with or without CCCP for 16 h. Cell lysates were subjected to immunoblotting analysis using antibodies for Tom20, parkin, HDAC6, and actin."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "MEFs were transfected with control or cortactin RNAi and parkin-GFP, and incubated with or without CCCP for 16 h as indicated. (A) Cells were subjected to immunostaining with antibodies for Tom20 and parkin. Bar, 10 µm."
NCBI gene IDs:
"cortactin: 13043
parkin: 56816"
Figure legend: "MEFs were transfected with control or cortactin RNAi and parkin-GFP, and incubated with or without CCCP for 16 h as indicated.(B) Cell lysates were subjected to immunoblotting with antibodies for Tom20, parkin, cortactin, and actin."
NCBI gene IDs:
"cortactin: 13043
parkin: 56816"
Figure legend: "(D-F) THP-1 cells stably expressing the nucleocapsid gene or control vector were stimulated with 10 μM nigericin for 30 min following pretreatment with 1 μg/ml LPS for 3 h. Supernatants and cell pellets were collected and  immunoblotted  with antibodies against the indicated proteins (D). The percentage of viable cells was determined through checking cellular ATP  levels in cell pellets (E). The percentage of cells undergoing pyroptosis  was calculated through checking released  LDH in the supernatants (F). For E, F, Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; NS, non-significant. Data information: control vector used here was empty vector corresponded to the nucleocapsid-containing vector. Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; NS, non-significant. Similar results were observed for at least three times."
NCBI gene IDs:
"nucleocapsid: 43740575"
Figure legend: "(D) GSDMD+/+ and GSDMD-/- THP-1  cells were transfected with plasmids encoding nucleocapsid for 24 h, followed by cell fixation, permeabilization and stain  with antibodies against nucleocapsid  and GSDMD. The nucleus was counterstained  with DAPI. Scale bar: 5 μm."
NCBI gene IDs:
"GSDMD: 79792
nucleocapsid: 43740575"
Figure legend: "(E, F) THP-1  (E) or   human CD14+ monocytes  (F) were transfected with plasmids encoding  FLAG-tagged nucleocapsid  for 36 h, followed by pretreatment of 1 μg/ml  LPS  for 3 h and 10 μM nigericin stimulation for 30 min. Cells were lysed and  immunoprecipitated with antibody against FLAG or isotype IgG. Input and precipitates were immunoblotted  as indicated."
NCBI gene IDs:
"FLAG: 
nucleocapsid: 43740575"
Figure legend: "(A, B) THP-1  cells stably expressing  nucleocapsid or control vector were stimulated with 10 μM  nigericin for 30 min following pretreatment with 1 μg/ml LPS for 3 h. Cells were either immunoblotted  with antibodies against the indicated proteins (A) or stained with propidium iodide (PI) (B, left). Scale bar: 20 μm. The percentage of PI positive cells was calculated (B, right). Student's t-test was used and data were shown as means±SD of three technical replicates. ***, P&lt;0.001; NS, non-significant. Results were repeated for three times with similar results. Data information: control vector used here was empty vector corresponded to the  nucleocapsid-containing vector. Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; ***, P&lt;0.001; NS, non-significant. Similar results were observed for at least three times."
NCBI gene IDs:
"nucleocapsid: 43740575"
Figure legend: "(H, I) THP-1  cells stably expressing full-length  nucleocapsid  or mutant lacking amino acids 70-160 and 290-360 were stimulated with 10 μM nigericin  for 30 min following pretreatment with 1 μg/ml LPS for 3 h. Cells were either immunoblotted  with antibodies against the indicated proteins (H). Cells were also stained with  PI  and the percentage of  PI  positive cells was calculated (I). Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; NS, non-significant. Results were repeated for three times with similar results. Data information: control vector used here was empty vector corresponded to the  nucleocapsid-containing vector. Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; ***, P&lt;0.001; NS, non-significant. Similar results were observed for at least three times."
NCBI gene IDs:
"nucleocapsid: 43740575"
Figure legend: "GSDMD-/- THP-1  cells rescued with wild-type or linker substituted GSDMD were transfected with plasmids encoding  nucleocapsid  or control vector and then stimulated with 10 μM nigericin  for 30 min following pretreatment with 1 μg/ml  LPS for 3 h. Cells were  immunoblotted  with antibodies against the indicated proteins (A)"
NCBI gene IDs:
"GSDMD: 79792
nucleocapsid: 43740575"
Figure legend: "(E, F) GSDMD-/- THP-1 cells rescued with wild-type or linker substituted GSDMD  were infected with SARS-CoV-2 at an MOI of 1 for 1h, followed by washing away extracellular viruses and further culture for 3 h. Otherwise, cells were stimulated with 10 μM nigericin  for 30 min following pretreatment with 1 μg/ml LPS for 3 h. Supernatants were subjected to an ELISA assay to determine  IL-1β levels (E). Otherwise, cell viability in cell pellets was examined using a cell viability assay  through checking  ATP levels inside cells (F). For E, F, Student's t-test was used and data were shown as means±SD of three technical replicates. **, P&lt;0.01; ***, P&lt;0.001; NS, non-significant. Results were repeated for three times with similar results."
NCBI gene IDs:
"GSDMD: 79792"
Figure legend: "C Quantitative RT-PCR detection of glycolytic gene expression levels in HaCaT cells upon miR-31 overexpression. β-actin was used as a house-keeping control gene (n=3 biological replicates)"
NCBI gene IDs:
"β-actin: 60
miR-31: 407035"
Figure legend: "G and H Glycolysis stress test of HaCaT cells upon UK5099 (G, n=4 biological replicates) or PC siRNA (si-PC, H, n=5 biological replicates) in 1mM glucose condition. ECAR and OCR were normalized to the amount of protein."
NCBI gene IDs:
"PC: 5091"
Figure legend: "B Western blot analysis of GLS, GS, GOT1, GOT2, AGC1 in HaCaT cells upon miR-31 overexpression, Tubulin α was used as loading control."
NCBI gene IDs:
"miR-31: 407035"
Figure legend: "C Quantitative RT-PCR detection of Has-miR-31-5p expression levels in HaCaT cells exposed to a range of glucose (n=4 biological replicates). RNU6-1 was used as house control gene."
NCBI gene IDs:
"Has-miR-31-5p: 407035
RNU6-1: 26827"
Figure legend: "D Western blot analysis of GS in HaCaT cells with miR-31 overexpression under different concentrations of glucose or 2-DG treatment, and Tubulin α was used as a loading control."
NCBI gene IDs:
"miR-31: 407035"
Figure legend: "A Representative picture of in situ hybridization (ISH) staining of miR-31 in skin biopsies of healthy individuals and psoriasis patients (n=5 individuals in each group). Quantification of results showing the average level of miR-31 in skin epidermis (right panel). B, basal layer; S+G, spinous and granular layers. Scale bars: 100 μm."
NCBI gene IDs:
"miR-31: 407035"
Figure legend: "J Quantitative RT-PCR detection of mmu-miR-31-5p expression levels in skin of mice (n=5 mice in each group)."
NCBI gene IDs:
"mmu-miR-31-5p: 723895"
Figure legend: "• A Assessment of proteolytic activity present in culture media of human embryonic kidney 293T cells transfected with cDNA encoding PAPP-A2, PAPP-A2(D643fs) (family 1), or PAPP-A2(A1033V) (family 2). Radiolabeled substrates tested include IGFBP-3, IGFBP-4, and IGFBP-5. Cleavage was visualized by autoradiography following SDS-PAGE. Positions of intact IGFBPs (i) and cleavage products (c) are indicated."
NCBI gene IDs:
"PAPP-A2: 60676"
Figure legend: "•C Comparison of proteolytic activity similar to the experiment of panel A, except that equimolar concentrations of PAPP-A2 and PAPP-A2 (A1033V) were used. The variant from Family 1, carrying a frameshift mutation N-terminal to the proteolytic domain of PAPP-A2, did not show any detectable expression. All gels and blots are representative of three independent experiments."
NCBI gene IDs:
"PAPP-A2: 60676"
Figure legend: "(d) An FP assay using FITC-labelled Galbi peptides, including mutants (L374A, Y380F and Y380A) against an increasing concentration of GAL8. (e) An FP assay using a FITC-labelled Galbi peptide against an increasing concentration of GAL8 mutants (E268A, I270A, Y272A and Trp317). (d,e) The KD values were calculated from at least three independent experiments. ND, not determined."
NCBI gene IDs:
"Galbi: 10241
GAL8: 3964"
Figure legend: "Immunoblotting and (D) quantification of the stability of four STOP target proteins in both G1S and natural mitotic cells treated with BCL9 siGenome pools and CHX (n=3)."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "Representative pictures (left panel) and the normalised biosensor intensity based on live cell imaging of the cells with a GSK3-GFP biosensor following different treatments before and after metaphase (n=10). Scale bars represent 10 μm."
NCBI gene IDs:
"GFP: 
GSK3: "
Figure legend: "Myc and β-catenin target gene enrichment analysis based on the transcriptome targets regulated by BCL9."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "The representative xenograft tumours (top panel) and tumour growth analysis (bottom panel) of scramble and BCL9 stable knockdown cells. LF: left flank of mouse, RF: right flank. n=6 for shS1 vs. shB1, n=5 for shS1 vs. shB2."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "Confocal (left panel) and live cell imaging (right panel) analysis of chromosome segregation in H2BGFP-cells following siRNA treatment; yellow arrows indicate the typical abnormal chromosomes by BCL9 knockdown. 23 H2BGFP-mitotic cells were monitored for each time experiment; data are shown from three independent experiments. Scale bars represent 5 μm. Quantification of aberrant chromosome segregation rate by live cell imaging of H2BGFP cells upon Wnt3a or DKK1 treatment. 23 H2BGFP-mitotic cells were monitored for each time experiment; data are shown from three independent experiments."
NCBI gene IDs:
"GFP: 
BCL9: 607
H2B: 128312///114483833///255626///8339///85236///158983///8340///3017///8341///8344///8342///8345///8348///8343///3018///8346///440689///100101478///337874///100288742///100820735///102724334///767811///54145///10340///337872///338391///337875///337873///286436///8970///8347///8349
H2B: 114483833///255626///128312///8339///85236///158983///8340///3017///8341///8344///8342///8345///8348///8343///3018///440689///8346///337874///100288742///100101478///100820735///767811///54145///10340///102724334///337872///338391///337875///337873///286436///8970///8347///8349
H2B: 114483833///255626///128312///8339///85236///158983///8340///8341///3017///8344///8342///8345///8348///8343///3018///440689///8346///337874///100288742///100101478///54145///100820735///767811///10340///102724334///338391///337872///337875///337873///286436///8970///8347///8349"
Figure legend: "Quantification of the duration of NEB to ANA (NEB: nuclear envelope breakdown, ANA: anaphase), NEB to cell death in interphase after cell division (blue colour bar) or in mitosis (dark red bar), or growth well in 30 hours (pink bar) by live cell imaging analysis in monitored H2BGFP cells with different treatments."
NCBI gene IDs:
"GFP: 
H2B: 114483833///255626///128312///8339///85236///158983///8340///3017///8341///8344///8342///8345///8348///8343///3018///440689///8346///337874///100288742///100101478///767811///100820735///54145///10340///102724334///337872///338391///337875///337873///286436///8970///8347///8349"
Figure legend: "Duolink analysis (A) and its quantification (B) of the interaction by assessing the PLA complex in mitotic cells after BCL9 or Axin1 knockdown (n=20). Scale bars represent 5 μm."
NCBI gene IDs:
"Axin1: 8312
BCL9: 607"
Figure legend: "Immunoprecipitation analysis of the BCL9-MF interaction in CLTC knockdown mitotic cells."
NCBI gene IDs:
"CLTC: 1213"
Figure legend: "Immunoprecipitation analysis of the endogenous clathrin interaction with LRP6 signalosome components in BCL9 MF-overexpressing mitotic cells."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "Immunoblotting analysis of signalosome components by immunoblotting in BCL9, CLTC or in both BCL9 and CLTC knockdown mitotic cells with CHX. Quantification of the stability of several LRP6 signalosome components by immunoblotting of mitotic cells with CHX."
NCBI gene IDs:
"BCL9: 607
CLTC: 1213"
Figure legend: "Immunoprecipitation analysis of transiently-expressed BCL9-MF or T172A-MF interactors in synchronised cells, from G1S to G2 M phase. The relative level of BCL9-MF or T172A-MF immunoprecipitation is determined; red numbers indicate up-regulation, and green numbers indicate down-regulation compared to the starting time point."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "Immunoprecipitation analysis of the transiently-expressed BCL9-MF interaction in mitotic cells treated with RO3306."
NCBI gene IDs:
"BCL9: 607"
Figure legend: "Log-rank (Mantel-Cox) survival analysis based on high and low p-T172-BCL9 expression scored by immunohistochemical staining in two tissue arrays with (G) 75 lung cancer samples or (H) 145 breast cancer samples."
NCBI gene IDs:
"BCL9: 607"
Figure legend: " (B) HeLa-GFP-Parkin cells were transfected with siNDP52 or scrambled siRNA. Seventy-two hours after transfection, cells were incubated with 1 µM valinomycin for the indicated times. Western blotting was performed using the indicated antibodies. Data are representative of three independent experiments "
NCBI gene IDs:
"NDP52: 10241"
Figure legend: " (D) Fluorescence images of NDP52 KD and control cells expressing mCherry-LC3 and MTS-TagBFP. Arrows indicate mCherry-LC3 puncta colocalized with MTS-TagBFP. Scale bars, 10 µm. Boxed areas in images are shown in the next panels on the right. Images are representative of four independent experiments "
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(E) Mitophagic flux in NDP52 KD cells expressing siRNA-resistant NDP52 or NDP52 mutants. Cells were incubated with valinomycin for 3 h. Results are the summary of three independent experiments. Values are the means ± SEM. *p &lt; 0.05, **p &lt; 0.01 vs scrambled oligo-transfected cells; †p &lt; 0.05, ††p &lt; 0.01 vs empty vector-transfected cells, determined with one-way ANOVA followed by the Tukey-Kramer post hoc test"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(F) HeLa-GFP-Parkin cells expressing mCherry-NDP52 mutants were incubated with 1 µM valinomycin and 100 nM bafilomycin A1 for 3 h. Cell lysates were subjected to immunoprecipitation using anti-RFP magnetic beads. Immunoprecipitates were analyzed by western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(G) Images of HeLa GFP-Parkin cells expressing mCherry-NDP52∆LIM-L or mCherry-NDP52∆SKICH treated with valinomycin for 2h. The images are from Movie EV 4. Cyan, GFP-Parkin; magenta, mCherry-NDP52 mutant. Scale bars, 10 µm. Images are representative of three independent experiments"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(A) Mitophagic flux in cells transfected with siNDP52, siTBK1, siOPTN or a combination of siRNAs. Results are the summary of three independent experiments. Values are the means ± SEM. **p &lt; 0.01 vs scrambled oligo-transfected cells; †p &lt; 0.05 vs siNDP52-transfected cells; §p &lt; 0.05, §§p &lt; 0.01 vs siTBK1; NS: not significant, determined with one-way ANOVA followed by the Tukey-Kramer post hoc test"
NCBI gene IDs:
"NDP52: 10241
OPTN: 10133
TBK1: 29110"
Figure legend: "(B) HeLa-GFP-Parkin cells transfected with siNDP52, siOPTN, siTBK1 or scrambled siRNA were incubated with 1 µM valinomycin for the indicated times. Cell lysates were subjected to SDS-PAGE or Phos-tag-PAGE prior to western blotting using the indicated antibodies. Data are representative of two independent experiments. The position of phosphorylated forms of NDP52 or OPTN are indicated by arrowheads"
NCBI gene IDs:
"NDP52: 10241
OPTN: 10133
TBK1: 29110"
Figure legend: "(C) Images of TBK1 KD cells expressing mCherry-NDP52 or mCherry-OPTN under valinomycin treatment from Movie EV 5. Cyan, GFP-Parkin; magenta, mCherry-NDP52. Images are representative of two independent experiments. Scale bars, 10 µm"
NCBI gene IDs:
"TBK1: 29110"
Figure legend: "(D) TBK1 KD and control cells expressed mCherry-NDP52. Lysates were subjected to immunoprecipitation using anti-RFP beads. Immunoprecipitates were analyzed by western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"TBK1: 29110"
Figure legend: "Anti-RFP immunoprecipitates from mCherry-NDP52 and mCherry-NDP52 ∆SKICH-transfected cells were subjected to SDS-PAGE followed by silver staining. Four bands specific for mCherry-NDP52 are numbered and were identified by mass spectrometric analysis. Asterisk (*) and double asterisk (**) indicate mCherry-NDP52 and mCherry-NDP52∆SKICH bands, respectively"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(D) HeLa-GFP-Parkin cells transfected with siMTPAP were incubated with 1 µM valinomycin for the indicated times. Western blotting was performed using the indicated antibodies. Data are representative of three independent experiments"
NCBI gene IDs:
"MTPAP: 55149"
Figure legend: "(E) Mitophagic flux in cells transfected with siNDP52, siMTPAP and combinations of siRNAs. Cells were incubated with valinomycin for 3 h. Results are the summary of three independent experiments. Values are the means ± SEM. **p &lt; 0.01 vs scrambled oligo-transfected cells; NS: not significant, determined with one-way ANOVA followed by the Tukey-Kramer post hoc test"
NCBI gene IDs:
"NDP52: 10241
MTPAP: 55149"
Figure legend: "(F) Images of MTPAP KD cells expressing mCherry-NDP52 under valinomycin treatment. The images are from Movie EV 7. Cyan, GFP-Parkin; magenta, mCherry-NDP52. Images are representative of two independent experiments. Scale bars, 10 µm"
NCBI gene IDs:
"MTPAP: 55149"
Figure legend: "(A, B) mCherry-LC3 and MTS-TagBFP were expressed in MTPAP KD and control cells. Cells were incubated with valinomycin for the indicated times and immunostained with anti-NDP52 antibody. Images are representative of two independent experiments. Yellow arrows indicate co-localization of MTS-TagBFP, mCherry-LC3 and NDP52. White arrows indicate co-localization of MTS-TagBFP and NDP52 (not mCherry-LC3). Scale bars, 10 µm. Co-localization of mCherry-LC3 and NDP52 on mitochondria is quantified in (B). At least ten cells from two independent experiments were quantified. Values are the means ± SEM. **p &lt; 0.01 vs control, determined with one-way ANOVA followed by the Student&quot;s t"
NCBI gene IDs:
"MTPAP: 55149"
Figure legend: "MTPAP KD and control cells expressed mCherry-NDP52 or empty vector (C Lysates from cells treated with 1 µM valinomycin and 100 nM bafilomycin A1 for 2 h were subjected to immunoprecipitation using anti-RFP beads. Immunoprecipitates were analyzed by western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"NDP52: 10241
MTPAP: 55149"
Figure legend: "MTPAP KD and control cells expresse mCherry-LC3 (D). Lysates from cells treated with 1 µM valinomycin and 100 nM bafilomycin A1 for 2 h were subjected to immunoprecipitation using anti-RFP beads. Immunoprecipitates were analyzed by western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"MTPAP: 55149"
Figure legend: "(E) MTPAP KD and control cells were incubated with valinomycin and bafilomycin A1 for 2 h. Cell lysates were subjected to immunoprecipitation using an anti-NDP52 antibody or control IgG. Immunoprecipitates were analyzed by western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"MTPAP: 55149"
Figure legend: "(F) GST-NDP52 conjugated with glutathione-Sepharose resin was incubated with purified recombinant LC3 and MTPAP∆MTS∆C. The pulled down complex was subjected to western blotting. Data are representative of three independent experiments"
NCBI gene IDs:
"NDP52: 10241
LC3: 81631///84557
MTPAP: 55149"
Figure legend: " (A) HeLa-GFP-Parkin cells expressing mCherry-NDP52 D439R/C443K mutant or WT NDP52 were incubated with valinomycin and nM bafilomycin A1 for 3 h. Cell lysates were subjected to immunoprecipitation using anti-RFP magnetic beads. Immunoprecipitates were analyzed by western blotting. Data are representative of two independent experiments "
NCBI gene IDs:
"NDP52: 10241"
Figure legend: " (B) Selected frames from time-lapse images of HeLa GFP-Parkin cells expressing mCherry-NDP52 D439R/C443K mutant under valinomycin and bafilomycin A1 treatment. The images are representative of two independent experiments and correspond to the boxed regions of Movie EV 9. Cyan, GFP-Parkin; magenta, mCherry-NDP52 D439R/C443K. The white arrowheads indicate colocalization of GFP-Parkin and mCherry-NDP52. The yellow arrowheads indicate mCherry-NDP existing inside GFP-Parkin ring structures. Scale bars, 1 µm"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "α, β, indicate Atg16L1 isoform α, β; ∆CCD indicates Atg16L1∆CCD; asterisk indicates a non-specific band. Atg16L1∆/∆ indicates mice expressing ∆Atg16L1. The number of endogenous LC3 or GFP-Atg5 dots was counted (c, d). The results shown are mean + s.d. (n &gt; 20)."
NCBI gene IDs:
"Atg16L1: 77040"
Figure legend: "c, IL-1b and IL-6 production by wild-type or Atg7-deficient macrophages stimulated with LPS. Statistical significance was determined by the Student's t-test. *P,0.01."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "e, f, Atg16L1-deficient chimaeric mice given 5% DSS in drinking water were intraperitoneally injected with both anti-IL-1b and anti- IL-18 neutralizing antibodies (squares; n55) or isotype control IgG (circles;n55) at days 1, 3, 5 and 7. The survival (e) and weight loss (f) of each mouse genotype were plotted."
NCBI gene IDs:
"Atg16L1: 77040"