EMBO/SourceData huggingface dataset:
Figure legend: "(E) H&amp;E staining of the skin in Sirt7f/f and Sirt7f/f;K15-Cre mice at P100."
NCBI gene IDs:
"Cre: 2777477
K15: 16665
Sirt7: 209011"
Figure legend: "(F) Representative immunofluorescence images showing Ki67/Pcad expression (upper) and Cd34/Ki67 expression (lower) in Sirt7fl/fl and Sirt7f/f;K15-Cre HFs on P56, P74 and P85. The white dashed line indicates bulge of hair follicle."
NCBI gene IDs:
"Cre: 2777477
K15: 16665
Sirt7: 209011"
Figure legend: "(A) Representative colonies formed by Sirt7+/+, Sirt7-/- and Sirt7-TG bulge SCs that were puriﬁed from telogen-phase HFs and allowed to grow for 2 weeks. (B-C) Quantiﬁcations of colony number and size in (A); n = 3 mice per genotype. "
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "Immunoblot analysis of the indicated proteins in HFSC colonies from Sirt7+/+, Sirt7-/- and Sirt7-TG mice (D)"
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "proteins in HFSC colonies from Sirt7+/+, Sirt7-/- and Sirt7-TG mice Nfatc1 protein levels were quantified using Image J Plus 6.0 (E, n = 3 mice for each group)."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(F) Immunoblot analysis of the NFATc1 expression level in human keratinocyte HaCaT cells treated with SIRT7 siRNAs."
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "(G) Immunoblot analysis of the NFATc1 expression in the presence of ectopic SIRT7 expression in HaCaT cells."
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "(H) NFATc1 luciferase activity was monitored in HEK293T cells after the indicated treatments (n=3). The relative luciferase activity was determined by dual luciferase reporter assay."
NCBI gene IDs:
"NFATc1: 4772"
Figure legend: "(I-J) IHC analyses of Nfatc1 expression in hair follicles of Sirt7f/f, Sirt7f/f ;K15-Cre and Sirt7-TG mice at the indicted time points. The black dashed line indicates the bulge (Bu) of hair follicle."
NCBI gene IDs:
"Cre: 2777477
K15: 16665
Sirt7: 209011"
Figure legend: "(K-L) qPCR analysis of Nfatc1-targeted genes in HFSCs colonies cultured from Sirt7+/+, Sirt7-/-and Sirt7-TG mice, n=3 for each genotypes."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(A) Representative immunoblots showing NFATc1 degradation in HEK293T cells stably overexpressing NFATc1 in the presence of cycloheximide (CHX), with or without SIRT7 overexpression. (B) Quantification of NFATc1 levels in (A) from 3 independent experiments. "
NCBI gene IDs:
"NFATc1: 4772
SIRT7: 51547"
Figure legend: "(C) Immunoblots of Nfatc1 protein levels in Sirt7+/+ and Sirt7-/- keratinocytes. (D) Quantification of Nfatc1 levels in (C) from 3 independent experiments. "
NCBI gene IDs:
"Sirt7: 51547"
Figure legend: "(E-F) Immunoblots (E) and related quantitative data (F, n=3) showing NFATc1 protein levels in HEK293T cells overexpressing wildtype HA-SIRT7 or a SIRT7 enzyme-dead mutant (H187Y)."
NCBI gene IDs:
"HA: 
SIRT7: 51547"
Figure legend: "(G-H) Immunoblots (G) and quantitative data (H, n=3) showing NFATc1 levels in HEK293T cells transfected with NFATc1-CA, with or without ectopic SIRT7, treated with MG132."
NCBI gene IDs:
"NFATc1: 4772
SIRT7: 51547"
Figure legend: "Immunoblots showing the interaction (by co-immunoprecipitation) between ectopic NFATc1/PA28γ in HEK293T cells (K) (L) after manipulating SIRT7 levels."
NCBI gene IDs:
"NFATc1: 4772
PA28γ: 10197
SIRT7: 51547"
Figure legend: "Immunoblots showing the interaction (by co-immunoprecipitation) between endogenous NFATc1/PA28γ in HaCaT cells (L) after manipulating SIRT7 levels."
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "(M-N) Immunoblot (M) and quantitative data (N, n=3) analysis of NFATc1 levels upon overexpression of HA-SIRT7 with or without PA28γ oligo siRNAs treatment."
NCBI gene IDs:
"HA: 
PA28γ: 10197
SIRT7: 51547"
Figure legend: "(O) Co-immunoprecipitation between NFATc1 and PA28γ in HEK293T cells cotransfected with WT HA-SIRT7 or an enzyme-dead mutant (H187Y)."
NCBI gene IDs:
"HA: 
SIRT7: 51547"
Figure legend: "(A) IHC staining of Nfatc1 expression in HFs on P84 in Sirt7+/+ and Sirt7-/- mice with or without Cyclosporin A (CsA) treatment. The black dashed line indicates bulge (Bu) of hair follicle. (B) Quantitative analysis of Nfatc1 levels in (A, n=10 hair follicles) using ImageJ plus 6.0. "
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(C) Immunofluorescent staining of Ki67 and Pcad at P84 in Sirt7+/+ and Sirt7-/- HFs with or without CsA treatment."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(E) Images were captured of the hair coats of Sirt7+/+ and Sirt7-/- mice at 15 months. The yellow dashed lines indicate hair thinning or hair loss."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(F) Immunofluorescence and IHC staining of Nfatc1 and Sirt7 in HFs of 15-month-old Sirt7+/+ and Sirt7-/- mice. The white/black dashed line indicates bulge (Bu) of hair follicle."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(H) Images captured of the skin in the plucked regions (blue dotted lines) of WT (3 months), aged WT (20 months) and aged Sirt7-TG mice (20 months), 14 days after depilation. Pigmentation of the skin indicates hair regrowth. n = 3 mice per genotype."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(I) Hair re-growth scores were analyzed for young WT, old WT and old Sirt7-TG mice in (H), n=3 mice for each group."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(J) The hair coat recovery score (described in methods) after the back hair of old WT and old Sirt7-TG mice was shaved. n = 3 mice per genotype."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(K) Images captured of the hair coats of WT and Sirt7-TG mice at 28 months-of-age. The yellow dashed lines indicate the hair thinning or hair loss regions."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "C. qPCR analysis of SATB2 mRNA expression in GSCs and matched non-stem tumor cells (NSTCs) (n=5)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "F. qPCR analysis of SATB2 mRNA expression in GSCs and neural progenitor cells (NPCs) (n=3)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "A. Immunoblot analysis of SATB2 expression in GSCs transduced with lentiviral-mediated non-targeting shRNA (shNT) or SATB2 shRNA (shSATB2)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "B. Cell viability of GSCs transduced with shNT or shSATB2 (n=5)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "C. EdU incorporation assay of GSCs transduced with shNT or shSATB2. Scale bar: 50 μm. D. Quantification of (C) showing the percentage of EdU+ cells (n=5). "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "E. Tumorsphere images of GSCs transduced with shNTor shSATB2. Scale bar: 100 μm. Quantification of the diameter of tumorspheres formed by GSCs expressing shNT or shSATB2 (F: n=9; G: n=5)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "G. Quantification of the number of tumorspheres formed by GSCs expressing shNT or shSATB2 (F: n=9; G: n=5)."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "H. In vitro limiting dilution analysis of the tumorsphere formations of GSCs expressing shNT or shSATB2. Silencing SATB2 attenuated the self-renewal capacity of GSCs."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " A. Bioluminescent images of the GBM xenografts derived from the luciferase-labeled T3359 GSCs expressing NT or SATB2 shRNA. Representative images on day 21 posttransplantation are shown (n=5 mice per group). Silencing SATB2 significantly delayed GBM growth . B. Quantification of the bioluminescence of xenografts derived from the luciferase-labeled T3359 GSCs expressing shNT or shSATB2 on day 21 posttransplantation (n=5 mice per group)."
NCBI gene IDs:
"luciferase: 
SATB2: 23314"
Figure legend: " C. Kaplan-Meier survival curves of mice intracranially implanted with T3359 GSCs expressing shNT or shSATB2 (shNT: n=7 mice; shSATB2-1 or shSATB2-2: n=5 mice). Median survival: shNT, 28 days; shSATB2-1, 48 days; shSATB2-2, 45 Days. Animals bearing GSC-derived xenografts expressing SATB2 shRNA survived longer than the control animals. "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " D. Immunofluorescence of SATB2 (Green) in xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=5 tumors per group). Scale bar: 40 μm. E. Quantification of SATB2 intensity in xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=5 tumors per group). "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " F. Immunofluorescence of Ki67 (Green) in tumor xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=5 tumors per group). Scale bar: 40 μm. G. Quantification of Ki67 positive cells in xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=5 tumors per group). "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " H. Immunofluorescence of SOX2 (Red) in xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=4 tumors per group). Scale bar: 40 μm. I. Quantification of SOX2 positive cells in xenografts derived from T3359 GSCs expressing shNT or shSATB2 (n=4 tumors per group). "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " A. Heatmap analysis of differentially expressed genes between SATB2 silencing H2S GSCs (shSATB2) and control H2S GSCs (shNT). Differentially expressed genes had a 1.8-fold or greater expression difference. Among differentially expressed genes, 160 are upregulated and 185 are downregulated. "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "C. qPCR analysis of FOXM1 and SATB2 mRNA expression in GSCs transduced with shNT or shSATB2 (n=3)."
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: "D. Immunoblot analysis of FOXM1 and SATB2 expression in GSCs transduced with shNT or shSATB2."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: "E. Immunoblot analysis of FOXM1 and SATB2 expression in tumor xenografts-derived from T3359 GSCs expressing shNT or shSATB2."
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " F. Heatmap analysis of FOXM1 downstream targets between SATB2 silencing H2S GSCs (shSATB2) and control H2S GSCs (shNT) from microarray analysis. Differentially expressed genes had a 1.5-fold or greater expression difference. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " G. qPCR analysis of FOXM1 downstream targets in H2S GSCs transduced with shNT or shSATB2 (n=3). "
NCBI gene IDs:
"SATB2: 23314"
Figure legend: " A. Immunoblot analysis of FOXM1 and SATB2 expression in T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " B. Cell viability assay of T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (n=5). Ectopic expression of FOXM1 restored the cell proliferation impaired by SATB2 silencing. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " C. Tumorsphere number of T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (n=5). Ectopic expression of FOXM1 restored the tumorsphere formation of GSCs impaired by SATB2 silencing. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " In vivo bioluminescent images of the tumor xenografts derived from luciferase-labeled T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=5 mice; shNT+FOXM1: n=4 mice; shSATB2: n=5 mice; shSATB2+ FOXM1: n=5 mice). Representative images on day 21 posttransplantation are shown. Ectopic expression of FOXM1 in GSCs expressing shSATB2 markedly restored GBM tumor growth. "
NCBI gene IDs:
"luciferase: 
FOXM1: 2305
SATB2: 23314"
Figure legend: " E. In vivo bioluminescent images or quantification of the tumor xenografts derived from luciferase-labeled T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=5 mice; shNT+FOXM1: n=4 mice; shSATB2: n=5 mice; shSATB2+ FOXM1: n=5 mice). Representative images on day 21 posttransplantation are shown. Ectopic expression of FOXM1 in GSCs expressing shSATB2 markedly restored GBM tumor growth. "
NCBI gene IDs:
"luciferase: 
FOXM1: 2305
SATB2: 23314"
Figure legend: " F. Kaplan-Meier survival curves of mice intracranially implanted with T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=8 mice; shNT+FOXM1: n=7 mice; shSATB2: n=6 mice; shSATB2+ FOXM1: n=8 mice). Median survival: shNT, 30 days; shNT+FOXM1, 28 days; shSATB2, 50.5 Days; shSATB2+FOXM1, 34 days. Ectopic expression of FOXM1 in GSCs expressing shSATB2 markedly attenuated the increased survival of mice bearing the GSC-derived GBMs. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " G. Immunofluorescence of Ki67 (Green) in xenografts derived from T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=6 tumors; shNT+FOXM1: n=5 tumors; shSATB2: n=6 tumors; shSATB2+ FOXM1: n=6 tumors). Scale bar: 40 μm. H. Quantification of Ki67 positive cells in xenografts derived from T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=6 tumors; shNT+FOXM1: n=5 tumors; shSATB2: n=6 tumors; shSATB2+ FOXM1: n=6 tumors). "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " I. Immunofluorescence of SOX2 (Red) in xenografts derived from T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=6 tumors; shNT+FOXM1: n=5 tumors; shSATB2: n=6 tumors; shSATB2+ FOXM1: n=6 tumors). Scale bar: 40 μm. J. Quantification of SOX2 positive cells in xenografts derived from T3359 GSCs transduced with FOXM1 or vector control in combination with shNT or shSATB2 (shNT: n=6 tumors; shNT+FOXM1: n=5 tumors; shSATB2: n=6 tumors; shSATB2+ FOXM1: n=6 tumors). "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " B. ChIP assays with the SATB2 antibody or IgG using GSCs, NSTCs and NPCs. PCR primers amplified a fragment flanking the MAR of FOXM1 gene locus. Note that abundant SATB2 binds to the MAR of FOXM1 gene locus in GSCs. "
NCBI gene IDs:
"FOXM1: 2305"
Figure legend: " C. qPCR analysis of ChIP assays with the SATB2 antibody or IgG using GSCs transduced with shNT or shSATB2 (n=3). PCR primers amplified a fragment flanking the MAR of FOXM1 gene locus. Silencing SATB2 decreased its binding amount to the MAR of FOXM1 gene locus. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " E. qPCR analysis of ChIP assays with the CBP antibody or IgG using GSCs transduced with shNT or shSATB2 (n=3). PCR primers amplified a fragment flanking the MAR of FOXM1 gene locus. Silencing SATB2 reduced the binding of CBP to the MAR of FOXM1 gene locus. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " F. qPCR analysis of ChIP assays with the indicated antibody (AcH3K18, AcH3K27, AcH4) using T3359 GSCs transduced with shNT or shSATB2 (n=3). PCR primers amplified a fragment flanking the MAR of FOXM1 gene locus. Silencing SATB2 reduced acetylation of H3K18, H3K27 and H4 levels on the MAR of FOXM1 locus. "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: " G. qPCR analysis of FOXM1 mRNA expression in T3359 GSCs transduced with shSATB2 or shCBP or both (n=3). "
NCBI gene IDs:
"CBP: 1387
FOXM1: 2305
SATB2: 23314"
Figure legend: " H. Immunoblot analysis of FOXM1 expression in T3359 GSCs transduced with shSATB2 or shCBP or both. "
NCBI gene IDs:
"CBP: 1387
SATB2: 23314"
Figure legend: " C. qPCR analysis of SATB2, FOXM1 and FOXM1 downstream targets in T3359 GSCs treated with indicated doses of C646 or the vehicle control for 24 hours (n=3). "
NCBI gene IDs:
"FOXM1: 2305
SATB2: 23314"
Figure legend: "E. Bioluminescent imaging of tumor growth in mice bearing xenografts derived from the luciferase-labeled T3359 GSCs treated with C646 or the vehicle control at indicated days after GSC transplantation (n=5 mice per group). F. Quantification of tumor growth from (E) (n=5 mice per group)."
NCBI gene IDs:
"luciferase: "
Figure legend: "Co-IP from 293T cells 48 h after transfection. RhoJ-PlexinD1 binding was disrupted by deleting the RBD or ECD of PlexinD1 and by 30-min stimulation with Sema3E. Arrows indicate PD1 Full (upper, 250 kDa) and PD1△RBD (lower, 1.7 kDa smaller than PD1 Full)."
NCBI gene IDs:
"PlexinD1: 67784"
Figure legend: "Immunoblots in siRNA-transfected HUVECs. Note the early and transient activation of PLCγ, Erk1/2, and Akt, but not p38 MAPK, in RhoJ-knockdown ECs."
NCBI gene IDs:
"RhoJ: 57381"
Figure legend: "Quantification of the kinetics of EC migration. Values of forward and reverse migration for RhojWT/WT are normalized to 1. n = 33 vessel branches per group."
NCBI gene IDs:
"Rhoj: 80837"
Figure legend: "Quantification of vessel elongation (left graph). n = 33 vessel branches per group. Scatter plot shows relationship between &quot;vessel elongation&quot; and &quot;reverse migration&quot; in RhojGFP/GFP aortic rings as evaluated by a Pearson correlation coefficient."
NCBI gene IDs:
"Rhoj: 80837"
Figure legend: "Labeling for YFP/GFP (green), CD31 (red), and ETS transcription factor ERG (blue) in retinal vascular fronts of P5 CAG-MerCreMer:R26R-EYFPflox/WT and CAG-MerCreMer:Rhojfloxflox mice after intraperitoneal (i.p.) injections of 10 µg of 4-hydroxytamoxifen (4OHT) at P1. Scale bar, 50 µm.   Relative contribution of YFP/GFP-positive ECs to the tip positions. n = 16 per group. Values for CAG-MerCreMer:R26R-EYPflox/WT are normalized to 1.   "
NCBI gene IDs:
"ETS: 
Rhoj: 80837"
Figure legend: "Labeling for GFP (green) and CD31 (red) in retina of P4 RhojGFP/GFP mouse. Scale bar, 200 µm (left); 20 µm (right)."
NCBI gene IDs:
"Rhoj: 80837"
Figure legend: "Labeling for EdU (green), ERG (red), and CD31 (gray) in retinas of P4 RhojWT/WT and RhojGFP/GFP mice 2 h after i.p. EdU injections. Scale bar, 20 µm. The graph shows EC proliferation index in retinal capillaries behind the angiogenic fronts. n = 12 per group."
NCBI gene IDs:
"GFP: 
Rhoj: 80837"
Figure legend: "Labeling for CD31 in retinas of P4 and P7 RhojWT/WT and RhojGFP/GFP mice. Scale bars, 500 µm."
NCBI gene IDs:
"GFP: 
Rhoj: 80837"
Figure legend: "Morphometric analyses of retinal vessels in RhojWT/WT (P4, n = 18; P7, n = 8) and RhojGFP/GFP (P4, n = 16; P7, n = 11) mice."
NCBI gene IDs:
"GFP: 
Rhoj: 80837"
Figure legend: "Labeling for CD31 in retinas of P4 Rhojflox/flox and Pdgfb-iCreERT2:Rhojflox/flox (RhojiΔEC) mice after single i.p. injection of 100 µg of 4OHT at P1. Scale bar, 500 µm."
NCBI gene IDs:
"iCreERT2: 
Pdgfb: 18591
Rhoj: 80837"
Figure legend: "Morphometric analyses of retinal vessels in P4 Rhojflox/flox (n = 7) and Rhoji∆EC (n = 6) mice."
NCBI gene IDs:
"Rhoj: 80837"
Figure legend: "Labeling for GFP (green) and CD31 (red) in retina of P18 OIR-RhojGFP/WT mouse. Scale bar, 200 µ"
NCBI gene IDs:
"GFP: 
Rhoj: 80837"
Figure legend: "Labeling for CD31 in retinas of P18 OIR-Rhojflox/flox and OIR-Rhoji∆EC mice after daily i.p. injection of 200 µg of 4OHT from P12. Scale bar, 500 µm. Quantification of areas of neovascular tufts in P18 OIR-Rhojflox/flox and OIR-Rhoji∆EC mice. n = 6 per group."
NCBI gene IDs:
"Rhoj: 80837"
Figure legend: "(B-C) HEK293 cells were transfected with empty vector or plasmids encoding HA tagged KDF1 and/or GST-tagged IKKα. Immunoprecipitation (α-HA or α-GST) was carried out to determine their interaction. Immunoprecipitates (IP) and whole cell lysate (WCL) were immunoblotted (IB) with different antibodies as indicated."
NCBI gene IDs:
"GST: 
HA: 
IKKα: 1147
KDF1: 69073"
Figure legend: "(E) HEK293 cells were transfected to co-express HA-tagged KDF1 with GST-tagged IKKα or its kinase dead (KD) mutant. IP and WCL were blotted with different antibodies as indicated."
NCBI gene IDs:
"GST: 
HA: 
IKKα: 1147
KDF1: 69073"
Figure legend: "(F) HEK293 cells were transfected to co-express HA-tagged KDF1 with GST-tagged IKKα or its different truncation mutants (EV Fig. 1B). IP and WCL were blotted with different antibodies as indicated. Arrow denotes antibody heavy chain."
NCBI gene IDs:
"GST: 
HA: 
IKKα: 1147
KDF1: 69073"
Figure legend: "(G) HEK293 cells were transfected to co-express GST-tagged IKKα with HA-tagged KDF1 or its different truncation mutants (EV Fig. 1C). IP and WCL were blotted with different antibodies as indicated."
NCBI gene IDs:
"GST: 
HA: 
IKKα: 1147
KDF1: 69073"
Figure legend: "(A) H/E staining of E18.5 skin sections from WT, IKKα KO, and KDF1 KO mice. Dotted lines denote dermal-epidermal boundaries. Epi: epidermis, Der: dermis. Scale bar=50 μm. (B) E18.5 skin sections from WT, IKKα KO, and KDF1 KO mice were immunostained with different antibodies as indicated (Krt14: Keratin 14, Krt10: Keratin 10, Lor: Loricrin, β4: β4-integrin, CD104). Dotted lines denote dermal-epidermal boundaries. Epi: epidermis, Der: dermis. Scale bar=100 μm. "
NCBI gene IDs:
"IKKα: 12675
KDF1: 69073"
Figure legend: "A) Immunoblots of WCL from IKKα KO cells with rescued expression of HA-tagged IKKα or its mutant using different antibodies as indicated."
NCBI gene IDs:
"HA: 
IKKα: 12675"
Figure legend: "B) Immunoblots of WCL from KDF1 KO cells with rescued expression of KDF1 or its mutant using different antibodies as indicated. Expression f exogenous KDF1 was induced by doxycycline (DOX) at different concentration."
NCBI gene IDs:
"KDF1: 69073"
Figure legend: "E) Sections of regenerated skin developed from engrafted IKKα KO, KDF1 KO, and their rescued cells were immunostained with different antibodies as indicated. Dotted lines denote dermal-epidermal boundaries. Epi: epidermis, Der: dermis. Scale bar=50 μm."
NCBI gene IDs:
"IKKα: 12675
KDF1: 69073"
Figure legend: "(A) WCL from WT and KDF1 KO cells before and after calcium shift were immunoblotted with different antibodies as indicated. Lo: low calcium; Hi: high calcium."
NCBI gene IDs:
"KDF1: 69073"
Figure legend: "(B-C) WT and KDF1 KO keratinocyte were treated with 20nM cycloheximide (CHX). WCL was collected at 0, 30, 60, 90, 120 min post CHX treatment and subjected to immunoblotting with IKKα antibody (B). Band intensity is determined by densitometry and the amount of IKKα is calculated and quantified (C). Statistical analysis is conducted using 2-way ANOVA. N=3 (biological replicates). *, p&lt;0.05. Error bar represents S.D. (standard deviation)."
NCBI gene IDs:
"KDF1: 69073"
Figure legend: "(D-E) WT and KDF1 KO keratinocyte (right panels) or cells transfected with plasmid encoding exogenous IKKα (left panels) were treated with MG132 at 10µM for 6 hours, then were subjected to immunoprecipitation using anti-ubiquitin (Ub) antibody. IP and WCL were analyzed by immunoblots with α-IKKα antibody (D). Overall intensity of all ubiquitinated IKKα bands was determined by densitometry. Ratio of ubiquitinated IKKα was quantified and presented as bar graphs (E). Statistical analysis is conducted using unpaired Student's t-test. Error bar represents S.D. (standard deviation). N=3 (biological replicates). *, p&lt;0.05. Kd: kilodalton for molecular weight markers."
NCBI gene IDs:
"IKKα: 1147
KDF1: 69073"
Figure legend: "(F) IKKα protein level in KDF1 KO keratinocyte expressing WT KDF1 or KDF1 mutant, under both low and high calcium conditions, was examined and quantified by western blotting"
NCBI gene IDs:
"KDF1: 69073"
Figure legend: "(G) PiggyBac transposon was used to ectopically express HA-tagged IKKα in KDF1 deficient cells. WCL were collected and analyzed by immunoblots with different antibodies as indicated."
NCBI gene IDs:
"HA: 
IKKα: 1147
KDF1: 69073"
Figure legend: "(H) Overexpression of IKKα in KDF1 KO keratinocyte can restore normal skin stratification. Skin sections from grafted tissue were immunostained with different antibodies as indicated. Dotted lines denote dermal-epidermal boundaries. Epi: epidermis, Der: dermis. Scale bars = 50 µm."
NCBI gene IDs:
"IKKα: 1147
KDF1: 69073"
Figure legend: "A) HEK293 cells were transfected to co-express KDF1 with USP7. IP and WCL were blotted with different antibodies as indicated."
NCBI gene IDs:
"KDF1: 69073
USP7: 252870"
Figure legend: "C) WCL from WT and USP7 CRISPR KO cells were subjected to immunoblotting with different antibodies as indicated."
NCBI gene IDs:
"CRISPR: 
USP7: 252870"
Figure legend: "D) IKKα protein level in WT and USP7 KO keratinocyte were examined by immunoblotting before and after calcium shift."
NCBI gene IDs:
"USP7: 252870"