EMBO/SourceData huggingface dataset:
Figure legend: "A-B. Representative 2D projections of confocal  images showing the expression  of  Ci-155 (blue and grey channels), ptc-GFP  (green and grey; ptc-GFP  is a bona-fide transcriptional read out of Hh-pathway and weak hypomorph allele and Castor  (red and grey) in germaria  with either normal (A) or deficient Dronc expression  (B). Genotypes: 109-30-Gal4  (BL7023)/ptc-GFPCB02030 (a gift from Isabel Guerrero) (A) and 109-30-Gal4  (BL7023)/ptc-GFPCB02030; DroncKOTub-G80ts (BL7019)/ UAS-flippase DroncKO-FRT-Dronc-GFP-APEX-FRT-QF. Data information: Scale bars represents 10 µm in the entire figure. Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels)."
NCBI gene IDs:
"flippase: 
GFP: 
Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "D. Western blot showing  Caspase-9 expression (upper lane) and actin  (bottom lane, loading control) in either control or Caspase-9 deficient  OVCAR-3 cells (24h and 72h post-transfection of an shRNA against Caspase-9). Notice the strong downregulation of Caspase-9 72h after siRNA treatment."
NCBI gene IDs:
"Caspase-9: 842"
Figure legend: "H. Quantification of total number of follicular cells (left) or Castor-expressing cells (right) within the FasIII  cellular domain in the following genotypes from left to right: Dronc  -/- = CTRL= 109-30-Gal4  (BL7023)/; DroncKOTub-G80ts (BL7019)/ UAS-flippase  (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=16). CTRL= 109-30-Gal4  (BL7023)/UAS-smoAct  (BL44621); DroncKOTub-G80ts (BL7019)/+ (n=11). Dronc  -/- = 109-30-Gal4  (BL7023)/UAS-smoAct (BL44621); DroncKOTub-G80ts (BL7019)/ UAS-flippase  (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=8). CTRL= 109-30-Gal4  (BL7023)/UAS-Ci  (BL28984); DroncKOTub-G80ts (BL7019)/+. (n=7). Dronc  -/- = 109-30-Gal4  (BL7023)/UAS- UAS-Ci (BL28984); DroncKOTub-G80ts (BL7019)/ UAS-flippase  (BL8209)  DroncKO-FRT-Dronc-GFP-APEX-FRT-QF (n=21). A Kruskal-Wallis test and Dunn's multiple comparison post-test were used to determine statistical significance (n.s.= not significant; ****p≤0.0001;). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). Unless otherwise indicated, all experimental data presented were obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of  germline cells wrapped by follicular cells."
NCBI gene IDs:
"flippase: 
GFP: 
Ci: 43767
Dronc: 39173
Gal4: 855828
smo: 33196
Tub: 40554"
Figure legend: "J. Quantification of total number of follicular cells (left) or Castor-expressing cells (right) within the FasIII  cellular domain in the following genotypes from left to right: Dronc  +/+ = ptc-Gal4  (BL2017)/+; Tub-G80ts (BL7019) (n=19). Dronc  +/- = ptc-Gal4  (BL2017)/+; DroncKOTub-G80ts (BL7019)/+ (n=23). Dronc  +/- UAS-Dronc  = ptc-Gal4  (BL2017)/+; DroncKOTub-G80ts (BL7019)/UAS-Dronc  (BL56198) (n=14). A one-way ordinary ANOVA and Welch's correction were used to establish statistical significance (n.s.= not significant; ****p≤0.0001). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). Unless otherwise indicated, all experimental data presented were obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of germline cells wrapped by follicular cells."
NCBI gene IDs:
"Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "B. Relative number of Ptc-positive puncta per germaria  of the following genotypes, from left to right: DroncKO Tub-G80ts /+ (n=10). ptc-Gal4  /+; Tub-G80ts/+ (n=9). ptc-Gal4  /+; DroncKO Tub-G80ts /+ (n=10). A Kruskal-Wallis test and Dunn's multiple comparison post-test were used to determine statistical significance (***p≤0.001 Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of germline cells wrapped by follicular cells."
NCBI gene IDs:
"Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "C. Western blot  showing Ptc  (upper lane) and actin  (bottom lane, loading control) expression in ovaries  of the genotypes shown in A. Notice the Ptc  accumulation in double heterozygous germaria (ptc-Gal4  /+; DroncKO Tub-G80ts /+)."
NCBI gene IDs:
"Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "E. Pearson colocalization  index between Ptc  and Atg8  in germaria  of the following genotypes: ptc-Gal4  (BL2017)/ UAS-GFP-mCherry-Atg8  (BL37749) (n=12). ptc-Gal4  (BL2017)/UAS-GFP-mCherry-Atg8  (BL37749); DroncKOTub-G80ts (BL7019)/+ (n=18). An unpaired parametric Welch's t-test was used to establish the statistical significance (** p≤0.01). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of germline cells wrapped by follicular cells."
NCBI gene IDs:
"Atg8: 
GFP: 
mCherry: 
Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "B. Total number of Atg8+ autophagosomes  in germaria  of the following genotypes ptc-Gal4  (BL2017)/ UAS-GFP-mCherry-Atg8  (BL37749) (n=21). ptc-Gal4  (BL2017)/ UAS-GFP-mCherry-Atg8  (BL37749); DroncKOTub-G80ts (BL7019)/+ (n=28). An unpaired parametric Welch's t-test was used to establish the statistical significance (* p≤0.05). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of germline cells wrapped by follicular cells."
NCBI gene IDs:
"Atg8: 
GFP: 
mCherry: 
Dronc: 39173
Gal4: 855828
ptc: 35851
Tub: 40554"
Figure legend: "H. Western blot showing the expression levels of the autophagy marker p62  (upper lane), Caspase-9 (middle lane) and Actin  (bottom lane, loading control) in either scrambled or Caspase-9  deficient  OVCAR-3 cells; the protein levels of the different read outs were measured at 72h after siRNA treatment in cells grown during the last 4 h before sample processing in our standard cell culture conditions, in cell culture media containing EtOH  (0.2%), and in cell culture media containing EtOH  (0.2%) +  bafilomycin A1  (400nM)."
NCBI gene IDs:
"Caspase-9: 842"
Figure legend: "E. Liposomes were treated with Dln1M5 or Dln1 of different concentrations at a fixed pH value of 5.5."
NCBI gene IDs:
"Dln1: 503710"
Figure legend: "C&amp;amp;amp;D. Transmission electron microscope micrograph of dispersed ring-like structures of Dln1M5 or Dln1D135A oligomers on lipid monolayer. The inset represents a few 2D class averages showing the octamer pattern from some individual rings."
NCBI gene IDs:
"Dln1: 503710"
Figure legend: "(A-D) Primary cultures from D. melanogaster embryos expressing GFP-Atg8a under the control of Dmef2-Gal4, stained with Phalloidin (actin) in red. Inset are magnifications of areas denoted by dotted line. (A) In untreated control cultures, GFP-Atg8a localizes throughout the muscle cytoplasm and nuclei. (B) Addition of rapamycin (Rap) for 12 hrs results in the formation of small GFP-Atg8a labeled punctae. (C) Addition of chloroquine (CQ) for 12 hrs results in the accumulation of large GFP-Atg8a labeled vesicles that localize around the nucleus and between the myofibers. (D) Addition of both CQ and Rap for 12hrs, triggers the formation of greater numbers of enlarged vesicles in the muscles than either drug alone. (E) Knockdown of the core autophagy gene, Atg1, prevents the formation of GFP-Atg8a vesicles in CQ+Rap treated cultures. (F) Quantification of the mean ratio of GFP-Atg8a punctae area to total muscle area per well (n = 4). Error bars indicate SEM with **p&lt;.01; ***p&lt;.001."
NCBI gene IDs:
"Gal4: 
Atg1: 39454
Dmef2: 36032"
Figure legend: "(A-D) Primary cultures from D. melanogaster embryos expressing GFP-Atg8a under the control of Dmef2-Gal4, stained with Phalloidin (actin) in red. Inset are magnifications of areas denoted by dotted line. (A) In untreated control cultures, GFP-Atg8a localizes throughout the musclecytoplasm and nuclei. (B) Addition of rapamycin (Rap) for 12 hrs results in the formation of small GFP-Atg8a labeled punctae. (C) Addition of chloroquine (CQ) for 12 hrs results in the accumulation of large GFP-Atg8a labeled vesicles that localize around the nucleus and between the myofibers. (D) Addition of both CQ and Rap for 12hrs, triggers the formation of greater numbers of enlarged vesicles in the musclesthan either drug alone. (E) Knockdown of the core autophagy gene, Atg1, prevents the formation of GFP-Atg8a vesicles in CQ+Rap treated cultures. (F) Quantification of the mean ratio of GFP-Atg8a punctae area to total muscle area per well (n = 4). Error bars indicate SEM with **p&lt;.01; ***p&lt;.001."
NCBI gene IDs:
"Gal4: 
Atg1: 39454
Dmef2: 36032"
Figure legend: "(E) Control white RNAi muscles starved and treated with CQ accumulate GFP-Atg8a punctae, but very few colocalize with ubiquitin (Ubi). (F) Knockdown of Rpn1 in starved + CQ muscle strongly inhibits the formation of GFP-Atg8a punctae. The remainder colocalize with ubiquitin. (G) Quantification of the mean GFP-Atg8a punctae area and Mander's overlap coefficient for GFP-Atg8a and Ubi for genotypes shown in E and F. Error bars indicate SEM for n = 8 ventral longitudinal muscles from individual animals, with p-values for Rpn1 RNAi relative to white RNAi control (***p&lt;.001)."
NCBI gene IDs:
"Rpn1: 40174
white: 31271"
Figure legend: "(H, I) Autophagosome number is reduced, but autophagic flux is not significantly altered in starved Rpn1 vs. white RNAi muscles expressing GFP-mCherry-Atg8. (J) Flux measurement is shown on the right as the ratio of mCherry punctae to GFP-mCherry (n = 6). SEM is indicated."
NCBI gene IDs:
"Rpn1: 40174
white: 31271"
Figure legend: "(K) Anti-Atg8a Western blot of third instar muscle lysate from Dmef2-Gal4; UAS-RNAi animals. In fed white RNAi control, endogenous Atg8a appears as a single band (Atg8a-I). 6 hrs starvation induces lipid modification of Atg8a and the appearance of a second band (Atg8a-II). Knockdown of Atg1 or Rpn1 inhibits Atg8a modification, indicating reduced autophagy levels."
NCBI gene IDs:
"Gal4: 
Atg1: 39454
Dmef2: 36032
Rpn1: 40174
white: 31271"
Figure legend: "(A-D) Inhibition of the proteasome by overexpression of a temperature sensitive proteasome mutant (DTS). Dmef2-Gal4, UAS-GFP-Atg8a control and UAS-DTS; Dmef2-Gal4, UAS-GFP-Atg8a and animals were shifted to the restrictive temperature then starved and CQ treated 6 hrs prior to dissection. 18 hrs post-shift (PS), both control (A) and DTS muscles (B) accumulate GFP-Atg8a punctae, but the latter also accumulate ubiquitin (Ubi) aggregates. 48 hrs PS, control muscles (C) maintain strong autophagy induction, but DTS mutant muscles (D) have reduced GFP-Atg8a punctae accumulation, similar to the Rpn1 knockdown phenotype. (E) Quantification of the mean GFP-Atg8a punctae and Mander's overlap coefficient for GFP-Atg8a and Ubi for genotypes shown in A-D (SEM is indicated for n = 8 ventral longitudinal muscles from individual animals with p-values of DTS relative to control). Dark green = Dmef2-Gal4, UAS-GFP-Atg8a; Light green = UAS-DTS; Dmef2-Gal4, UAS-GFP-Atg8a."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
DTS: 39855"
Figure legend: "(F) Western blot analysis of endogenous Atg8a from third instar muscle lysate. 6 hrs starvation induces lipidated Atg8a-II in Dmef2-Gal4 control at both 18 hrs and 48 hrs PS, but not in DTS at 48 hrs PS."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
DTS: 39855"
Figure legend: "(A-C) Time course of effect of temperature shift on aggregates, autophagosomes, and larval locomotion in UAS-DTS; Dmef2-Gal4, UAS-GFP-Atg8a animals starved 6 hrs prior to analysis. (A) The number of ubiquitin aggregates peaks at 18-24 hrs PS, and decreases significantly from 18 to 48 hrs PS (n = 10 and p-values shown relative to 18 hrs PS). (B) The number of GFP-Atg8a punctae peaks at 12-24 hrs PS and decreases significantly from 18 to 48 hrs PS (n = 10 and p-values shown relative to 18 hrs PS). (C) Larval locomotion, measured as the time to crawl 3cm, gradually and significantly worsens from 18 to 48 hrs PS (n = 6 and p-values shown relative to 0 hrs PS."
NCBI gene IDs:
"Dmef2: 36032
DTS: 39855"
Figure legend: "(D-E) tyramine (Tyr) treatment reduces GFP-Atg8a punctae in larval muscle. UAS-DTS; Dmef2-Gal4, UAS-GFP-Atg8a animals were placed on 100mg/ml Tyr (D) or control food (E), shifted to restrictive temperature for 18hrs, and starved 6 hrs prior to dissection."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
DTS: 39855"
Figure legend: "(F-H) Effect of Tyr treatment on aggregates, autophagosomes, and larval locomotion in UAS-DTS; Dmef2-Gal4, UAS-GFP-Atg8a and Dmef2-Gal4, UAS-GFP-Atg8a animals, shifted to restrictive temperature for 18hrs, and starved 6 hrs prior to dissection. Aggregate (F) and GFP-Atg8a punctae (G) numbers in muscles are significantly reduced with 100 mg/ml Tyr treatment in DTS but not control animals 18 hrs post-shift (PS). (H) 100 mg/ml Tyr treatment significantly worsens larval locomotion in both control and DTS animals 18 hrs PS. n = 12 and p-values shown relative to non-Tyr treated animals. For all measurements, SEM is shown with *p&lt;.05; **p&lt;.01; ***p&lt;.001."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
DTS: 39855"
Figure legend: "(I) Western blot analysis of endogenous Atg8a from third instar muscle lysate 18 hrs PS. 6 hrs starvation induces lipidated Atg8a-II in Dmef2-G4 control with or without 100 mg/ml Tyr. After 6hrs starvation, Atg8a-II levels are reduced in DTS + 100 mg/ml Tyr relative to untreated DTS."
NCBI gene IDs:
"Dmef2: 36032
DTS: 39855"
Figure legend: "(D-H)Dmef2-Gal4 drives expression of UAS-GFP-Atg8a in animals starved on low nutrient food for 6 hrs + 2.5 mg/ml CQ for 6 hrs. RNAi knockdown of CG41099 (E), CG7112 (F), CG31935 (G), and Rab3-GAP (H), blocks the formation of GFP-Atg8a labeled autophagosomes compared to knockdown of white (D)."
NCBI gene IDs:
"Gal4: 
CG31935: 33338
CG41099: 3355072
CG7112: 38945
Dmef2: 36032
Rab3-GAP: 34626
white: 31271"
Figure legend: "(I-M) Dmef2-Gal4 drives expression of UAS-myc-Atg8a in animals starved on low nutrient food for 6 hrs + 2.5 mg/ml CQ for 6 hrs. The effect of the RAB3GAPs is not via Rab3 or Rab5 function, as a Rab3 null mutation (J), expression of a constitutive active form of Rab3 (Q80L) (K) or Rab5 (Q88L) (L) has no effect on muscle autophagy compared to control (I). Expression of a constitutive active form of Rab39 (Q69L) (M) reduces myc-Atg8a labeled vesicles. (N) Quantification of autophagy phenotypes from panels D-M. SEM is indicated, with n = 8 ventral longitudinal muscles from individual animals and **p&lt;.01; ***p&lt;.001."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
Rab3: 36127
RAB3GAP: 34626
Rab39: 31684
Rab5: 33418"
Figure legend: "(O) Atg8a and Rab3-GAP colocalize to autophagosomes. Dmef2-Gal4 drives expression of UAS-myc-Atg8a and UAS-CFP-Rab3-GAP in animals starved and treated with CQ for 6 hrs. In all panels green = GFP-Atg8a and red = myc-Atg8a, and blue = CFP-Rab3Gap2."
NCBI gene IDs:
"Gal4: 
Dmef2: 36032
Rab3-GAP: 34626"
Figure legend: "(P) Anti-Atg8a Western blot of third instar muscle lysate from Dmef2-Gal4 driving the RNAi or overexpression constructs shown in D-N. Total levels of endogenous Atg8a are variable, but only knockdown of CG41099, CG7112, CG31935, Rab3-GAP, or overexpression of Rab39 (Q69L) block induction of lipidated Atg8a-II following 6 hrs starvation."
NCBI gene IDs:
"Gal4: 
CG31935: 33338
CG41099: 3355072
CG7112: 38945
Dmef2: 36032
Rab3-GAP: 34626
Rab39: 31684"
Figure legend: "(A) Primary cultured muscles with control lacZ RNAi, treated for 12 hrs with Rap+CQ. Note the formation of large GFP-Atg8a vesicles throughout the muscles. (B) GFP-Atg8a vesicles are reduced in primary cultured muscles with GlyS RNAi, treated for 12 hrs with Rap+CQ. Knockdown of Pfk (C) or CG9485 (D) causes increased accumulation of GFP-Atg8a vesicles in primary cultured muscles treated for 12 hrs with Rap+CQ. (E) Quantification for A-D of the mean ratio of GFP-Atg8a punctae area to total muscle area per well (n = 4). SEM is indicated with *p&lt;.05; **p&lt;.01."
NCBI gene IDs:
"CG9485: 37435
GlyS: 41823
Pfk: 36060"
Figure legend: "(F) Control white RNAi muscles accumulate large autophagosomes at 1 mg/ml CQ. (G) RNAi knockdown of CG9485 increases autophagosome accumulation in the muscle. (H) RNAi knockdown of AGBE reduces autophagic vesicle accumulation in the muscle. (I) Quantification of the GFP-Atg8a punctae area in muscles from white, CG9485, and AGBE knockdowns.n = 6 larval muscles with SEM indicated and *p&lt;.05."
NCBI gene IDs:
"AGBE: 326264
CG9485: 37435
white: 31271"
Figure legend: "(J) Western blot analysis of endogenous Atg8a from third instar muscle lysate. Dmef2-Gal4 driven knockdown of AGBE but not CG9485 inhibits Atg8a lipidation, indicating reduced autophagy levels."
NCBI gene IDs:
"Gal4: 
AGBE: 326264
CG9485: 37435
Dmef2: 36032"
Figure legend: "RFP+ (G1 phase), GFP+ (G2 phase), and GFP+/RFP+ (S phase)   RPE-FUCCI cells were sorted by flow cytometry, RNA was extracted and analyzed using qRT-PCR (n=3). Expression is normalized to GAPDH  mRNA. Bars show mean ± SD."
NCBI gene IDs:
"GFP: 
RFP: 
GAPDH: 2597"
Figure legend: "RPE cells transfected with control or FAM110A siRNA were fixed with 4 % PFA, probed with  rabbit polyclonal antibody against  FAM110A and mouse monoclonal to  γ-tubulin, and were analyzed by confocal microscopy. Shown is maximal projection of representative mitotic (upper panel) and interphase (lower panel) cells. Scale bars indicate 10 μm."
NCBI gene IDs:
"FAM110A: 83541"
Figure legend: "Quantification of the  mitotic index in three experiments from (A) and in  RPE cells transfected with siRNAs to FAM110B and CSPP1 that were analyzed in parallel. Shown is median ±SD (n=3). Statistical significance was determined by ANOVA (* p&lt;0.05, ** p&lt;0.005)."
NCBI gene IDs:
"CSPP1: 79848
FAM110B: 90362"
Figure legend: "RPE  cells transfected with control or FAM110A  siRNA were grown for 48 h and treated with MG132 30 min prior fixation to trap mitotic cells in metaphase. Impaired  chromosomal alignment and multipolar spindles were scored and representative  images are shown. Scale bars indicate 20 μm, arrowheads point to misaligned chromosomes. More than 30  metaphases were evaluated per condition (n=3). Bars indicate median ±SD. Statistical significance was determined by two-tailed t-test, (*** p&lt;0.001, * p&lt;0.05).   Quantification of lagging chromosomes  in anaphase  cells from (E). More than 30 anaphases were evaluated per condition (n=3). Representative images of abnormal anaphase are shown, scale bars indicate 20 μm, arrowheads point to incorrectly segregating chromosomes. Statistical significance was determined by two-tailed t-test. Bars indicate median ±SD (*** p&lt;0.001).   "
NCBI gene IDs:
"FAM110A: 83541"
Figure legend: "Cell extracts from asynchronously growing or mitotic  RPE-EGFP or RPE-EGFP-FAM110A cells were incubated with  GFP Trap  and bound proteins were analyzed by immunoblotting. Antibody against pS10H3 was used as a marker of mitosis."
NCBI gene IDs:
"EGFP: 
FAM110A: 83541"
Figure legend: "RPE cells or RPE cells stably expressing indicated EGFP-FAM110A variants were transfected with control (siNC), FAM110A  or CSNK1D siRNA and grown for 48 h. Alternatively, cells were incubated with PF670462  for the last 12 h. Cells were treated with MG132 for 30 min prior fixation. Impaired chromosomal alignment was scored in &gt;30 metaphase cells per condition. Error bars indicate median ±SD. Statistical significance was determined by ANOVA (n=3) (**** p&lt;0.0001, *** p&lt;0.001)."
NCBI gene IDs:
"EGFP: 
CSNK1D: 1453
FAM110A: 83541"
Figure legend: "Representative immunoblot analysis of samples from (D). Full arrow indicates migration of the EGFP-FAM110A-WT, empty arrow of endogenous FAM110A."
NCBI gene IDs:
"EGFP: 
FAM110A: 83541"
Figure legend: "RPE1 were transfected with control siRNA or CSNK1D  siRNA or were treated with PF670462  prior fixation. Cells were probed with rabbit polyclonal to FAM110A  and mouse monoclonal to γ-Tubulin. Representative  images are shown, scale bars indicate 10 μm.   Quantification of (A). Plotted is median FAM110A intensity at  spindle poles positive for  γ-tubulin ± SD. Each dot represents a single  spindle pole. Statistical significance was determined by ANOVA (n=3) (**** p&lt;0.0001).   "
NCBI gene IDs:
"CSNK1D: 1453"
Figure legend: "Cells expressing the wild-type or mutant EGFP-FAM110A were fixed and stained for γ-tubulin and α-tubulin and analyzed by  confocal microscopy (z-stack 0.16 µm). Representative images of maximal projections of the metaphase cells are shown, scale bars indicate 10 μm. Merged images show signal of DAPI,  γ-tubulin and  EGFP. Insets show a single Z plane at the spindle pole."
NCBI gene IDs:
"EGFP: 
FAM110A: 83541"
Figure legend: "A, RT-qPCR (n = 3-12 biological replicates) analyses of CGN  expression in different mouse  organs at P21."
NCBI gene IDs:
"CGN: 70737"
Figure legend: "B. MDCK  cells expressing WT or mutant CGN  were immunolabeled  with CGN  or Flag  antibodies."
NCBI gene IDs:
"CGN: 57530"
Figure legend: "D. RT-qPCR  of CGN  expression in transfected MDCK  cells (n = 3 biological replicates). Data information: Data are presented as mean ± SEM; unpaired Student's t-test was used in (D). ns, not significant, P &gt; 0.05."
NCBI gene IDs:
"CGN: 57530"
Figure legend: "A-C. DPOAE  thresholds (A), ABR  thresholds (B) and ABR  P1 amplitudes  (C) of the 2-month old control (Cgnfl/fl) and Cgn-cKO (Cgnfl/fl;Pou4f3creER/+) mice. Control mice  (circle), n = 16 mice  (female  = 8, male  = 8); Cgn-cKO mice  (square), n = 19 mice  (female  = 11, male  = 8). Data information: Data are presented as mean ± SEM; two-way ANOVA was used * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001 and **** P &lt; 0.0001."
NCBI gene IDs:
"Cgn: 70737
cre: 2777477
ER: 13983
Pou4f3: 18998"
Figure legend: "D-I. Growth curves of ABR  P1 amplitudes  at different cochlear  frequencies (5.6 - 32 kHz) of the 2-month old control and Cgn-cKO mice. Control mice  (circle), n = 16 mice  (female  = 8, male  = 8); Cgn-cKO mice  (square), n = 19 mice  (female  = 11, male  = 8). Data information: Data are presented as mean ± SEM; two-way ANOVA was used * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001 and **** P &lt; 0.0001."
NCBI gene IDs:
"Cgn: 70737"
Figure legend: "J. Whole mount immunofluorescence    of the organ of Corti   from 2-month old control and Cgn-cKO mice. Phalloidin  (F-actin, magenta); Myosin7a  (hair cells, green). White arrows label the lost OHCs."
NCBI gene IDs:
"Cgn: 70737"
Figure legend: "C. Whole mount immunofluorescence  of CGN expression  in organ of Corti   from P21 CgndelG mouse."
NCBI gene IDs:
"Cgn: 57530"