EMBO/SourceData huggingface dataset:
Figure legend: "A, TIRF  images of live KIF2A-GFP   or KIF2C-GFP  knock-in HeLa  cells transiently transfected with mCherry-KANK1  (A) Insets show enlargements of boxed areas. Data information: Scale bars: 5 μm."
NCBI gene IDs:
"GFP: 
mCherry: 
KANK1: 23189
KIF2A: 3796
KIF2C: 11004"
Figure legend: "Treatment of HeLa cells with DFP (1mM for 24 h) decreases levels of CHIP (n=8, biological replicates; ***p&lt;0.001; paired t-test).   Treatment of HeLa cells with DFP (1mM for 24 h) leads to decreased levels of CHIP mRNA (n=3, biological replicates; *p&lt;0.05; unpaired t-test).  "
NCBI gene IDs:
"CHIP: 10273"
Figure legend: "CHIP knockdown increases levels of SENP3 but not SENP5 in HeLa cells. Nsi or CHIPi (Nsi, non-specific siRNA; CHIPi, CHIP siRNA; concentration, 20 nM) was transfected into HeLa cells for 48 h. Lysate samples were blotted as indicated.   CHIP knockdown prevents the DFP-induced increase in SENP3 levels. Nsi, SENP3i or CHIPi (Nsi, non-specific siRNA; SENP3i, SENP3 siRNA; CHIPi, CHIP siRNA; siRNA concentration, 20nM) was transfected into HeLa cells for 48 h. Two days post-transfection the cells were treated with DFP for a further 24 h.  "
NCBI gene IDs:
"SENP3: 26168
CHIP: 10273"
Figure legend: "SENP3 knockdown abolishes the DFP-induced increase in LC3-II in HeLa cells. Nsi or SENP3i (I) was transfected into HeLa cells. Two days post-transfection the cells were treated with DFP for a further 24 h. Whole cell lysate samples were blotted as indicated (Nsi, non-specific siRNA; SENP3i, SENP3 siRNA; siRNA concentration, 20nM; DFP, 1mM for 24 h; values normalized to the control value; n=3, biological replicates; ***, p&lt;0.001; N.S., not significant, p&gt;0.05; paired t-test)."
NCBI gene IDs:
"SENP3: 26168"
Figure legend: "Nsi or SENP3i (II) was transfected into HeLa cells with stable expression of mito-Keima grown on 6-well plates. Two days post-transfection the cells were treated with DFP (1mM; 24 h). Cells transfected with Nsi and treated with CQ (50μM; 24 h) in the absence or presence of DFP (1mM; 24 h) were included as a negative control for mitophagy induction. The images show overlaid fluorescence and transmitted light images of unfixed, live cells obtained using an EVOS-fl inverted LED fluorescence microscope (Red: mitophagic puncta; Scale bar, 100 μm). Histogram shows the percentages of mitophagic cells under 6 different conditions (n=3, biological replicates; ****, p&lt;0.0001; Ordinary one-way ANOVA followed by Tukey's multiple comparisons test)."
NCBI gene IDs:
"SENP3: 26168"
Figure legend: "SENP3 knockdown abolishes DFP-induced mitophagic autolysosomes. Nsi or SENP3i (I) together with mito-pHfluorin were transfected into HeLa cells in the absence or presence of DFP (24 h), and were analyzed using the mito-pHfluorin construct 72 h post-transfection (Red: mCherry-A or puncta indicating occurrences of mitophagy marked by white arrows; Green: SEP-A; Yellow: mitochondria labelled by mCherry-SEP-A; Magenta: SENP3; Scale bar, 10 μm; n=28 cells per condition from two individual experiments; **** p&lt;0.0001; unpaired t-test)."
NCBI gene IDs:
"SENP3: 26168"
Figure legend: "Fis1 knockdown abolishes DFP-induced LC3-II. Fis1 siRNA was introduced into HeLa cells for 48 h, and the cells were treated with DFP for further 24 h (Nsi, non-specific siRNA; Fis1i, Fis1 siRNA; siRNA concentration, 20nM; DFP, 1mM). Whole cell lysate samples were blotted as indicated. Values are presented as mean ± SEM and are normalized to the control value (n=3, biological replicates; **, p&lt;0.01; paired t-test)."
NCBI gene IDs:
"Fis1: 51024"
Figure legend: "Fis1 knockout abolishes DFP-induced LC3-II. Fis1+/+ or Fis1-/- cells were treated with DFP for 24 h. Whole cell lysate samples were blotted as indicated. Values are presented as mean ± SEM and are normalized to the control value (n=6, biological replicates; *, p&lt;0.05; paired t-test)."
NCBI gene IDs:
"Fis1: 51024"
Figure legend: "Fis1 knockdown prevents DFP-induced mitophagic autolysosomes. Nsi or Fis1i together with mito-pHfluorin were transfected into HeLa cells in the absence or presence of DFP (24 h), which were analysed using the mito-pHfluorin construct 72 h post-transfection (Red: mCherry-A or puncta indicating occurrences of mitophagy marked by white arrows; Green: SEP-A; Yellow: mitochondria labelled by mCherry-SEP-A; Blue: Fis1; Scale bar, 10 μm; n=25 cells per condition from three individual experiments; *** p&lt;0.001; unpaired-t test)."
NCBI gene IDs:
"Fis1: 51024"
Figure legend: "Flag-Fis1 is deSUMOylated by SENP3. SENP3 knockdown enhances Fis1 SUMOylation. Nsi or SENP3i was transfected into HEK293 cells expressing Flag-Fis1, His-SUMO-2 and Ubc9 for 48 h. His-pulldown and lysate samples were detected by immunoblotting for Flag, SENP3 or β-actin. Values are presented as mean ± SEM (n=3, biological replicates; * p&lt;0.05; paired-t test)."
NCBI gene IDs:
"Flag: 
His: 
Fis1: 66437
SUMO-2: 59343
SENP3: 26168
Ubc9: 7329"
Figure legend: "The SUMOylation status of Fis1 influences its mitochondrial localization. Flag-Fis1 WT, Flag-Fis1 SUMOylation deficient mutant K149R or Flag-Fis1-SUMO-2ΔGG was expressed in HeLa cells for 48 h. Whole cell lysates and cytosolic and mitochondrial fractions were prepared and blotted as indicated (the upper panel). Histograms (the right panel) show normalized levels of Flag-Fis1 WT or Flag-Fis1 K149R mutant associated with the cytosolic or mitochondrial fraction (Value are presented as mean ± SEM and are normalized to the control value; n=7, biological replicates; *, p&lt;0.05; paired t-test)."
NCBI gene IDs:
"Flag: 
Fis1: 66437
SUMO-2: 59343"
Figure legend: "The SUMOylation status of Fis1 may influence its association with the Endoplasmic Reticulum (ER). Flag-Fis1 WT, Flag-Fis1 SUMOylation deficient mutant K149R or Flag-Fis1-SUMO-2ΔGG along with GFP-Cb5 as an ER marker were expressed in HeLa cells for 48 h. Immunocytochemistry was then performed (Green: ER labelled by GFP-Cb5; Blue: different Flag-Fis1 forms; Scale bar, 10 μm)"
NCBI gene IDs:
"Flag: 
Fis1: 66437
SUMO-2: 59343"
Figure legend: "Expressing a SUMOylation-deficient Fis1 mutant reverses the effect of SENP3 knockdown on DFP-induced LC3-II induction. pcDNA3, Flag-Fis1 WT or Flag-Fis1 K149R mutant was transfected into HeLa cells in which SENP3 was depleted using siRNA for 48 h (Nsi, non-specific siRNA; SENP3i, SENP3 siRNA (I); siRNA concentration, 20nM), and the cells were treated with DFP for a further 24 h (DFP, 1mM). Lysate samples were blotted as indicated."
NCBI gene IDs:
"Flag: 
Fis1: 66437
SENP3: 26168"
Figure legend: "SENP3 depletion does not abolish mitophagic puncta detected in HeLa cells expressing SUMOylation deficient CFP-Fis1 K149R in the presence of DFP. CFP-Fis1 WT or CFP-Fis1 K149R mutant were transfected into HeLa cells in which SENP3 was depleted using siRNA for 48 h, and the cells were treated with DFP for a further 24 h (Red: mCherry-A or puncta indicating occurrences of mitophagy marked by white arrows; Green: SEP-A; Cyan/blue: CFP-Fis1; Magenta, SENP3; Scale bar, 10 μm); Values are presented as mean ± SEM (n=21 cells per condition from two individual experiments; **** p&lt;0.0001; unpaired t-test). Knockdown of SENP3 was further confirmed by immunoblotting (the lower right panel)."
NCBI gene IDs:
"CFP: 
Fis1: 66437
SENP3: 26168"
Figure legend: "A, B: Quantitative RT-PCR results showing relative expression levels of mouse Cnot6 and Cnot6l in oocytes (GV and MII), somatic tissues, ES cells (A) and preimplantation embryos (B). n = 3 biological replicates"
NCBI gene IDs:
"Cnot6: 104625
Cnot6l: 231464"
Figure legend: "C: Cumulative numbers of pups per female showing fertility of WT and Cnot6l-/- female mice. n = 5 females for each genotype"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "D: Representative images results of oocytes collected from oviducts of WT and Cnot6l-/- mice at 16 h after hCG injection. scale bar, 100 μm. Arrows indicate polar body-1 (PB1)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "E: Confocal microscopy results of oocytes collected from oviducts of WT and Cnot6l-/- mice at 16 h after hCG injection. scale bar, 20 μm. Arrows indicate spindle poles"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "F: Rates of PB1 emission and normal spindle formation in oocytes ovulated by WT and Cnot6l-/- female mice. The numbers of analyzed oocytes were indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "G: Quantification of preimplantation embryos derived from WT and Cnot6l-/- females that develop to the indicated stages after hCG administration and mated with adult WT males. The numbers of analyzed embryos are indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "H: Representative images of embryos collected from the oviducts or uteri of WT and Cnot6l-/- female at indicated time points after hCG administration. Scale bar, 100 μm. Data information: Error bars, s.e.m. **P &lt; 0.01; ***P &lt; 0.001 by two-tailed Student&quot;s t test. n.s.: non-signif"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "A: Rates of germinal vesicle breakdown (GVBD) and PB1 emission in oocytes cultured in vitro. Fully-grown GV oocytes were collected from PMSG-primed (44 h) WT and Cnot6l-/- mice. PB1: polar body-1. Error bars, s.e.m. The numbers of analyzed oocytes were indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "B: Representative images of WT, Cnot6l-/- and Btg4-/- oocytes showing PB1 emission at 16 h after culture. Arrows indicate PB1. Scale bar, 100 μm"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "C: Representative images of chromosome spreads made from WT and Cnot6l-/- oocytes after 16 h of in vitro maturation culture. Immunofluorescent staining of topoisomerase II (TOP2) and the centromere antigen CREST were performed to indicate chromosome arms and centromeres, respectively. Numbers of paired sister chromatids are indicated. Scale bar, 5 μm"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "D: Percentage (%) of aneuploidy among in vitro cultured WT and Cnot6l-/- oocytes that have released PB1s. Error bars, s.e.m. *P &lt; 0.05; ***P &lt; 0.001 by two-tailed Student\"s t test. n.s.: non-significant. The numbers of analyzed oocytes were indicate"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "E: Rates of GVBD, PB1 emission, and normal spindle assembly in WT, Cnot6l-/- and Btg4-/- oocytes cultured in vitro. Error bars, s.e.m. ***P &lt; 0.001 by two-tailed Student\"s t test. n.s.: non-significant. The numbers of analyzed oocytes were indicate"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "F: Confocal microscopy results showing spindle assembly in WT, Cnot6l-/- and Btg4-/- oocytes at metaphase I (MI) and metaphase II (MII). Scale bar, 20 μm"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "G: Pericentrin immunofluorescence showing MTOCs in cultured WT, Cnot6l-/- and Btg4-/- oocytes at MI and MII stages. Spindle and DNA were labeled by microtubule nucleation factor (TPX2) and DAPI, respectively. Scale bar, 20 μm"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "A: Live cell imaging results showing in vitro meiotic division of WT and Cnot6l-/- oocytes. Abbreviations: PM, prometaphase; MI, metaphase I; AI, anaphase I; TI, anaphase I-metaphase II transition; MII, metaphase II; PB1: polar body-1. Hours after released from GV arrest were indicated. Scale bar, 20 μm"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "B: Data represent the mean and standard deviations of mCherry-Securin fluorescence intensity in WT and Cnot6l-/- oocytes at each time point. Values from individual oocytes are normalized relative to that at 0 h after released from GV arrest. The numbers of analyzed oocytes were indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "C: SMC3 immunofluorescence showing cohesin on chromosomes of WT and Cnot6l-/- oocytes at 16 h after culture. Centromeres and DNA were labeled by CREST and DAPI, respectively. Scale bar, 5 μm"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "D: BUB3 immunofluorescence on chromosome spreads made from WT and Cnot6l-/- oocytes at 9.5 h after culture. More than 8 oocytes were observed for each genotype with similar results. Scale bar, 5 μm"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "E: PB1 emission rates in WT and Cnot6l-/- oocytes cultured with or without MPS1 inhibitor reversin (5 μm). Reversin is added at 9 hours after culture. Error bars, s.e.m. The numbers of analyzed oocytes were indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "A: Relative mRNA copy number dynamics in WT and Cnot6l-/- samples at the indicated stages. Error bars indicate values of the two biological replicates"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "B: Box plot showing gene expression levels of WT, Cnot6l-/- and Btg4-/- oocytes at the MII stage. Genes were divided into 10 bins according to their expression levels in the WT MII oocytes. The box indicates upper and lower quantiles, the thick line in the box indicate the median, and the whiskers represent 2.5th and 97.5th percentiles. ***P &lt; 0.001 by two-tailed Student\"s t test. n.s.: non-signif"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "C: qRT-PCR results showing the relative expression levels of Cnot6l in oocytes collected from WT and Cnot6l-/- mice (Cnot6l-/-/WT). Error bars, s.e.m. ***P &lt; 0.001 by two-tailed Student\"s t test. n = 3 biological repli"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "D: Scatter plot comparing transcripts between WT and Cnot6l-/- oocytes (at GV, MI and MII stages) and zygotes derived from these oocytes. Transcripts decreased or increased more than five folds in Cnot6l-/- oocyte samples were high-lighted with blue or red, respectively"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "F: Relative mRNA copy number dynamics of the three gene clusters in WT, Cnot6l-/- and Btg4-/- samples"
NCBI gene IDs:
"Btg4: 56057
Cnot6l: 231464"
Figure legend: "Western blot results showing the levels of CPEB1 in WT and Cnot6l-/- oocytes at the indicated time points after meiotic resumption. Total proteins from 50 (A oocytes are loaded in each lane. DDB1 (A is blotted as a loading control. Experiments were performed three times with reproducible results; a representative result is shown"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "Western blot results showing the levels of CPEB1 in WT and Cnot6l-/- oocytes at the indicated time points after meiotic resumption. Total proteins fro 100 (B) oocytes are loaded in each lane α-tubulin (B) is blotted as a loading control. Experiments were performed three times with reproducible results; a representative result is shown"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "Immunofluorescenc of L-homopropargylglycine (HPG) showing the overall translation levels of MI and MII oocytes collected from WT and Cnot6l-/- mice. Scale bar, 20 μm. Error bars, standard deviations (n = 6 oocytes for each genotype). **P &lt; 0.01, ***P &lt; 0.001"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "quantification (D) of L-homopropargylglycine (HPG) showing the overall translation levels of MI and MII oocytes collected from WT and Cnot6l-/- mice. Scale bar, 20 μm. Error bars, standard deviations (n = 6 oocytes for each genotype). **P &lt; 0.01, ***P &lt; 0.001"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "E: Relative mRNA copy numbers of polysome-bound transcripts in WT and Cnot6l-/- oocytes at indicated stages. Error bars indicate values of the replicates"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "F: Scatter plot comparing polysome-bound transcripts between WT and Cnot6l-/- oocytes at GV, MI and MII stages, respectively. Transcripts decreased or increased more than five folds in Cnot6l-/- oocyte samples were high-lighted with blue or red, respectively"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "I: Quantitative RT-PCR results showing the relative levels of indicated transcripts in association with polysomes in WT and Cnot6l-/- oocytes at GV, MI and MII stages. Error bars, standard deviations (n = 3 biologica repeats)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "A: Presence of putative AREs (AUUUA) in transcripts of WT oocyte. Transcripts containing no ARE or transcripts containing 1 or more AREs were subdivided into frequency groups according to the log2 fold change (MII/GV) in WT oocytes"
NCBI gene IDs:
"ARE: "
Figure legend: "B: Presence of putative AREs in oocyte transcripts stabilized by Cnot6l-/- knockout. Transcripts containing no ARE or transcripts containing 1 or more AREs were subdivided into frequency groups according to the log2 fold change (Cnot6l-/-/WT) at MII stage"
NCBI gene IDs:
"ARE: 
AREs: 
Cnot6l: 231464"
Figure legend: "C: Co-IP results showing interaction of ZFP36L2 with CNOT6L and CNOT7. HeLa cells were co-transfected with plasmids expressing HA-ZFP36L2 and FLAG-CNOT6L/7 for 48 h before immunoprecipitation. Experiments were performed three times with reproducible results; a representative result is shown"
NCBI gene IDs:
"CNOT6L: 231464
ZFP36L2: 12193"
Figure legend: "D: RNA immunoprecipitation results showing interaction of CNOT6L with indicated transcripts, with or without the presence of ZFP36L2. HeLa cells were co-transfected with plasmids expressing FLAG-ZFP36L2 and HA-CNOT6L for 48 h before immunoprecipitation using an anti-HA antibody. mRNAs recovered from the immunoprecipitates were subjected to qRT-PCR. Error bars, standard deviations (n = 3 biological repeats). ***P &lt; 0.001 by two-tailed Student&quot;s t"
NCBI gene IDs:
"CNOT6L: 231464
ZFP36L2: 12193"
Figure legend: "fluorescence microscopy results (B) showing the translation activities of the Cnot6l 3\"-UTR (WT and CPE mutated) in GV- (maintained by 2 μM milrinone) or MII- (released from milrinone) arrested oocytes. GFP signal indicated translational activation of Cnot6l 3\"-UTR. An in vitro transcribed and polyadenylated mCherry mRNA was co-microinjected as a positive control. Scale b"
NCBI gene IDs:
"Cnot6l: 231464
CPE: 12877"
Figure legend: "GFP signal indicated translational activation of Cnot6l 3\"-UTR. An in vitro transcribed and polyadenylated mCherry mRNA was co-microinjected as a positive co Relative intensity of GFP signa after normalization by mCherry signal in the same oocyte. Error bars, s.e.m. *P &lt; 0.05; ***P &lt; 0.001 by two-tailed Student\"s t test. n.s.: non-significant. The numbers of analyzed oocytes were indicate"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "fluorescence microscopy results (E) showing the expression of GFP-fused Cnot6l 3&quot;-UTR in GV and MII oocytes with or without U0126 treatment (20 μM). Scale bar, 1"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "e expression of GFP-fused Cnot6l 3\"-UTR in GV and MII oocytes with or without U01 F: Relative intensity of GFP signa after normalization by mCherry signal in the same oocyte The numbers of analyzed oocytes were indicated (n)"
NCBI gene IDs:
"Cnot6l: 231464"
Figure legend: "G: Rates of PB2 emission and normal spindle assembly in oocytes cultured with or without U0126. Fully grown GV oocytes were microinjected with mRNAs encoding CNOT6L, CNOT7 and/or BTG4 and are released from milrinone at 12 h after microinjection. Then the oocytes were further culture for 24 h with or without adding U0126 to the medium. Error bars, s.e.m. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 by two-tailed Student&quot;s t test. n.s.: non-significant. The numbers of analyzed oocytes were indicate"
NCBI gene IDs:
"BTG4: 56057
CNOT6L: 231464
CNOT7: 18983"
Figure legend: "A Representative siliques from wild-type (WT; Col-0), herk1, anj and herk1 anj plants prior to dehiscence. Siliques were placed on double-sided sticky tape and carpel walls separated from the replum to expose the developing seeds. Scale bar = 5 mm."
NCBI gene IDs:
"anj: 836091
herk1: 823774"
Figure legend: "B Developing seeds per silique in wild-type, herk1, anj and herk1 anj plants. Fully expanded siliques were dissected and photographed under a stereomicroscope. n = 15 (four independent experiments with at least three plants per line and five siliques per plant). Data presented are means ± SEM. *** p&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"anj: 836091
herk1: 823774"
Figure legend: "C Percentage of pollen tubes with normal reception at the female gametophyte (black bars; representative image middle centre of figure) and with overgrowth (grey bars; representative image lower centre) as assessed by aniline blue staining. 15 self-pollinated stage 16 flowers from wild-type, herk1, anj and herk1 anj were analysed. Legend scale bars = 50 µm. *** p&lt;0.001 (χ-square tests)."
NCBI gene IDs:
"anj: 836091
herk1: 823774"
Figure legend: "D Aniline blue staining of pollen tube reception in reciprocal crosses between wild-type and herk1 anj plants with at least two siliques per cross. Legend as per (C). *** p&lt;0.001 (χ-square tests)."
NCBI gene IDs:
"anj: 836091
herk1: 823774"
Figure legend: "E,F Localisation of HERK1-GFP in the synergid cell from the pFER::HERK1-GFP construct in (F) and corresponding differential interference contrast (DIC) image in (E). White and red dotted lines delineate the egg cell and synergid cells, respectively. Data information : Scale bars = 50 µm. M, micropyle. Arrows, filiform apparatus."
NCBI gene IDs:
"GFP: 
FER: 824318
HERK1: 823774"
Figure legend: "G,H Localisation of ANJ-GFP in the synergid cell from the pANJ::ANJ-GFP construct in (H) and corresponding DIC image in (G). White and red dotted lines delineate the egg cell and synergid cells, respectively. Data information : Scale bars = 50 µm. M, micropyle. Arrows, filiform apparatus."
NCBI gene IDs:
"GFP: 
ANJ: 836091"
Figure legend: "A Localisation of HERK1, ANJ, LRE, FER and NTA in the synergid cell of wild-type (Col-0; WT), herk1 anj and lre-5 in unfertilised ovules, as shown by pFER::HERK1-GFP, pANJ::ANJ-GFP, pLRE::LRE-Citrine, pFER::FER-GFP and pMYB98::NTA-GFP. DIC and fluorescence images are shown, left to right, respectively. White and red dotted lines delineate the egg cell and synergid cells, respectively. Scale bars = 25 µm."
NCBI gene IDs:
"Citrine: 
GFP: 
anj: 836091
ANJ: 836091
FER: 824318
herk1: 823774
HERK1: 823774
LRE: 6240393
lre-5: 6240393
NTA: 816249
MYB98: 827611"
Figure legend: "B Localisation of NTA in the synergid cell of wild-type and herk1 anj plants before (upper panels) and after (lower panels) pollen tube arrival. In green, NTA localisation as shown by pMYB98::NTA-GFP fluorescence. In magenta, callose of the filiform apparatus and pollen tube stained with SR2200. From left to right, images shown are DIC, merged fluorescence images, and merged images of DIC and fluorescence. White and red dotted lines delineate the pollen tube and synergid cells, respectively. Scale bars = 25 µm. M, micropyle."
NCBI gene IDs:
"GFP: 
anj: 836091
herk1: 823774
NTA: 816249
MYB98: 827611"
Figure legend: "Profile of relative fluorescence intensity of NTA-GFP along the synergid cells of wild-type and herk1 anj ovules (C) before (virgin) and after (pollinated) pollen arrival. Data shown are means ± SEM, n = 25. *** p&lt;0.001 (Student's t-test). FA, filiform apparatus."
NCBI gene IDs:
"anj: 836091
herk1: 823774"
Figure legend: "D Profile of relative fluorescence intensity of NTA-GFP along the synergid cells of wild-type and lre-5 ovules (D) before (virgin) and after (pollinated) pollen arrival. Data shown are means ± SEM, n = 25. *** p&lt;0.001 (Student's t-test). FA, filiform apparatus."
NCBI gene IDs:
"lre-5: 6240393"
Figure legend: "A Representative images of ovules from wild-type (Col-0), herk1 anj, lre-5 and fer-4 20 hours after emasculation (HAE) displaying the mature female gametophyte structure. Images presented here are maximum intensity projections from confocal microscopy images across several z-planes of ovules stained as per [70]. Scale bars = 50 µm."
NCBI gene IDs:
"anj: 836091
fer-4: 824318
herk1: 823774
lre-5: 6240393"
Figure legend: "B Quantification of H2DCF-DA staining of ROS in ovules from wild-type, herk1 anj, lre-5 and fer-4 plants at 20 HAE. Categories are listed in the legend Ovules dissected from at least five siliques per line. *** p&lt;0.001 (χ-square tests)."
NCBI gene IDs:
"anj: 836091
fer-4: 824318
herk1: 823774
lre-5: 6240393"