EMBO/SourceData huggingface dataset:
Figure legend: "(H) Bar graph of qPCR  data measuring the relative levels of CRACR2B  mRNA in MCF10A MECs  ligated with rBM  in 2D  and 3D  12h post-plating (mean ± SEM; n=4 independent biological replicates). *P = 0.0371 (Student's t test)."
NCBI gene IDs:
"CRACR2B: 283229"
Figure legend: "(C) Representative fluorescence microscopy images of MCF10A MECs  co-expressing the ER-plasma membrane contact site reporter GFP-MAPPER  and shRNA targeting filamin  (FLMN) or luciferase (Luc) ligated to rBM  in 2D  for 18h. Scale bar (whole cell), 10 μm; Scale bar (magnified), 2 μm."
NCBI gene IDs:
"Luc: 
luciferase: 
filamin: 2316
FLMN: 2316"
Figure legend: "(D) Quantification of the levels of GFP-MAPPER  at the  plasma membrane versus total cellular fluorescence in MCF10A MECs  stably expressing an shRNA targeting filamin  (FLMN) or luciferase  (Luc) ligated to rBM  in 2D (mean ± SEM; 2D + shLuc, n=38; 2D + shFLMN , n=39 cells from three independent experiments). **P &lt; 0.0021 (Student's t test)."
NCBI gene IDs:
"Luc: 
luciferase: 
filamin: 2316
FLMN: 2316"
Figure legend: "(E) Representative immunoblots  of filamin, phosphorylated EIF2a  (pEIF2a), total EIF2a  and  alpha-tubulin in cell lysate from MCF10A MECs  that express shRNA targeting filamin  (shFLMN) or luciferase  (shLuc) and ligated to a rBM  in 2D  or 3D  for 18h and corresponding quantification data (mean ± SEM; n=4 independent biological replicates). Statistical analysis by one-way ANOVA followed by Uncorrected Fisher's LSD. 2D-shLuc versus 3D-shLuc, ***P = 0.0003. 2D-shLuc versus 2D-shFLMN, ***P = 0.0004."
NCBI gene IDs:
"Luc: 
luciferase: 
filamin: 2316
FLMN: 2316"
Figure legend: "(F) Bar graph of the relative levels of ATF3  mRNA as measured by qPCR  in MCF10A MECs  expressing shRNA targeting filamin  (shFLMN) or luciferase  (shLuc) and ligated with rBM  in 2D  or 3D  for 18h (mean ± SEM; n=4 independent biological replicates). Statistical analysis by one-way ANOVA followed by Uncorrected Fisher's LSD. 2D-shLuc versus 3D-shLuc, ***P = 0.0005. 2D-shLuc versus 2D-shFLMN, *P = 0.0359."
NCBI gene IDs:
"Luc: 
luciferase: 
ATF3: 467
filamin: 2316
FLMN: 2316"
Figure legend: "(G) Representative immunoblots  of filamin, phosphorylated EIF2a  (pEIF2a), total EIF2a  and alpha-tubulin in cell lysate from MCF10A MECs  ligated to rBM  in 3D and treated with ethanol  (mock) or doxycycline  (FLMN) for 18h to induce filamin   expression and corresponding quantification data (mean ± SEM; n=5 independent biological replicates). **P = 0.0018 (Student's t test)."
NCBI gene IDs:
"FLMN: 2316"
Figure legend: "(A) Schematic showing the principles behind  Atomic Force Microscopy  (AFM) (left). A cantilever at the end of the microscope probe is deflected when it is in contact with the cell surface. Cell cortex-mediated resistance to indentation alters the path of the laser beam focused on the cantilever that is then reflected onto a photodetector to enable measurement of cellular  cortical actin tension. AFM  was used to measure the  cortical actin tension in MCF10A MECs  ligated to a laminin-111  substrate in 2D  or 3D  (right) and treated with blebbistatin  (Bleb) to reduce cortical  actin  tension, induced to overexpress filamin  expression (FLMN), or activated ROCK  (ROCK) to increase  cortical actin tension. MECs  were indented using a 2-µm beaded tip on the AFM  cantilever and the Hertz model was used to fit each indentation curve to extract the Young's modulus of the cell cortex (mean ± SEM; 2D, n=123; 3D, n=184; 3D+FLMN, n=83; 2D+Bleb, n=66; 3D  + Bleb, n= 65; 3D+ROCK, n=212; n=AFM  indentation from &gt;30 cells from three independent experiments). Statistical analysis by one-way ANOVA followed by Tukey's multiple comparisons test. 2D  versus 3D, ****P &lt; 0.0001; 3D  versus 3D  + FLMN, ****P &lt; 0.0001; 3D  versus 3D  + ROCK, ****P &lt; 0.0001; 2D  versus 2D  + Bleb, ***P=0.0004."
NCBI gene IDs:
"filamin: 2316///2317///2318
FLMN: 2316///2318///2317
ROCK: 282041"
Figure legend: "(D) Schematic depicting strategy used to measure cortical  tension using  laser ablation. (Top) Cells with high cortical  tension exhibit plasma membrane blebbing  when cortical actin is severed by a pulsed laser, whereas cells with lower cortical  tension do not. (Bottom left) Bar graph of the laser ablation response of MCF10A MECs  ligated to a rBM  in 2D  or 3D  (mean ± SD; 2D, n= 35; 3D, n=40 cells from three independent experiments). (Bottom right) Bar graph showing the laser ablation response of MCF10A MECs  ligated to a rBM  in 2D or 3D  and treated in the absence or presence of blebbistatin  (2D+Bleb  and 3D+Bleb) or expressing constitutively active ROCK  (3D+ROCK) (mean; 2D, n= 14; 3D, n=15; 2D+Bleb, n=17; 3D+Bleb, n=14; 3D+ ROCK, n=10 cells from one experiment)."
NCBI gene IDs:
"ROCK: 282041"
Figure legend: "(D) Bar graph of qPCR  data measuring the relative levels of ATF3  mRNA in MECs  ligated with rBM  in 2D and treated in the absence or presence of blebbistatin  (Bleb) (mean ± SEM; n=4 independent biological replicates). *P = 0.0158 (Student's t test)."
NCBI gene IDs:
"ATF3: 467"
Figure legend: "(I) Representative line graph showing the changes in cytosolic  [Ca2+] levels of MCF10A MECs  in response to treatment. Fura-2-loaded MCF10A MECs  ligated with rBM  in 2D and expressing filamin  repeats 21-23 (2D+FLMNIg21-Ig23) or vector control (2D) were pre-incubated with EGTA  to chelate extracellular Ca2+ in the bath solution, challenged with 2 μM thapsigargin  to induce ER Ca2+ release, and replenished with 4 mM Ca2+ in bath solution at the indicated time. F340/F380 values of individual cells were quantified over the course of imaging (mean ± SD (2D, n=8; 2D + FLMNIg21-Ig23, n=7 cells from one experiment). (J) Quantification of the amplitude of the Ca2+ response (∆F=F-F0) induced by thapsigargin, where F0 is the basal fluorescence before thapsigargin  treatment. The data shown indicated mean ± SEM (2D, n=21; 2D + FLMNIg21-Ig23, n=19 cells from three independent experiments). ****P &lt; 0.0001 (Student's t test)."
NCBI gene IDs:
"FLMN: 2317///2316///2318
FLMN: 2318///2316///2317
filamin: 2318///2317///2316"
Figure legend: "(K) Bar graph of qPCR  data measuring the relative levels of ATF3  mRNA in MCF10A MECs  expressing luciferase  control or filamin  repeats 21-23 (FLMNIg21-Ig23) ligated with rBM  in 2D for 18h (mean ± SEM; n=3 independent biological replicates). *P = 0.032 (Student's t test)."
NCBI gene IDs:
"luciferase: 
ATF3: 467
filamin: 2316///2317///2318
FLMN: 2318///2316///2317"
Figure legend: "(L) Representative immunoblots  of phosphorylated EIF2a  (pEIF2a), total EIF2a  and alpha-tubulin  in cell lysate from MCF10A MECs  expressing luciferase  (control) or filamin  repeats 21-23 (FLMNIg21-Ig23-myc) that were ligated with rBM  in 2D. Corresponding quantification data are shown at bottom (mean ± SEM; n=5 independent biological replicates). *P = 0.0112 (Student's t test)."
NCBI gene IDs:
"luciferase: 
myc: 
filamin: 2316///2317///2318
FLMN: 2317///2316///2318"
Figure legend: "(A) Bar graph of qPCR  data measuring the levels of SEC61B  mRNA in MECs  ligated with rBM  in 2D and treated in the absence and presence of blebbistatin  (Bleb) (mean ± SEM; n=3 independent biological replicates). **P = 0.0046 (Student's t test)."
NCBI gene IDs:
"SEC61B: 10952"
Figure legend: "(B) Bar graph of the percent cell death measured in MCF10A MECs  stably expressing shRNAs targeting Luciferase  (Luc), filamin (FLMN) or actinin  (ACTN) and ligated to rBM  in 2D. Cell death  was assessed at 48h post-plating via calcein AM  and ethidium homodimer staining  (mean ± SEM; n =3 independent biological replicates). **P = 0.0034; ns= not significant (Student's t test)."
NCBI gene IDs:
"Luc: 
Luciferase: 
actinin: 81
ACTN: 81
filamin: 2316
FLMN: 2316"
Figure legend: "(C) Bar graph of the percent cell death in MCF10A MECs  expressing luciferase  control or filamin  repeats 21-23 (FLMNIg21-Ig23) that were ligated with rBM  in 2D. Cell death was assessed at 48h post-plating via calcein AM and ethidium homodimer staining  (mean ± SEM; n =3 independent biological replicates). *P = 0.0489 (Student's t test)."
NCBI gene IDs:
"luciferase: 
filamin: 2316///2317///2318
FLMN: 2316///2318///2317"
Figure legend: "(D) Bar graph of the levels of endogenous Exo70  in MECs  stably expressing shRNA against Exo70  or luciferase  (Luc) (mean ± SEM; n =3 independent biological replicates). ** p = 0.0086."
NCBI gene IDs:
"Luc: 
luciferase: 
Exo70: 23265"
Figure legend: "(E) Bar graph of the percent cell death measured in MECs  expressing shRNA targeting Exo70  (shExo70) or luciferase  (control) and ligated with rBM  in 2D  or 3D. Cell death was assessed at 48h post-plating via calcein AM  and ethidium homodimer staining  (mean ± SEM; n=3 independent biological replicates). Statistical analysis by one-way ANOVA followed by Uncorrected Fisher's LSD. 2D  versus 3D, ***P = 0.0004. 3D + shLuc versus 3D  + shExo70, **P = 0.0091."
NCBI gene IDs:
"Luc: 
luciferase: 
Exo70: 23265"
Figure legend: "(F) Spheroids were induced to express constitutively active ROCK  or left uninduced (control) and co-stained with antibodies for laminin  or S100A14  (green), phalloidin  (red) and DAPI  (blue). Scale bar, 10 μm."
NCBI gene IDs:
"ROCK: 282041"
Figure legend: "(G) Representative immunoblots  of phosphorylated EIF2a  (pEIF2a), total EIF2a  and alpha-tubulin  in cell lysate from MCF10A MEC  spheroids induced to express constitutively active ROCK  or left uninduced (control). Corresponding quantification data are plotted at bottom (mean ± SEM; n=3 independent biological replicates). *P = 0.0442 (Student's t test)."
NCBI gene IDs:
"ROCK: 282041"
Figure legend: "(H) Spheroids were induced to express constitutively active ROCK  or left uninduced (control), treated with Paclitaxel  (2nM), lysed and immunoblotted  for cleaved caspase-3  and alpha-tubulin  (top). In parallel, spheroids were stained  with antibody and phalloidin  to evaluate the levels of caspase-3 (green) and actin  organization (red), respectively. Scale bar, 10 μm."
NCBI gene IDs:
"ROCK: 282041"
Figure legend: "(I) Bar graph of the relative mRNA levels of ER  stress response genes including ATF3, STC2, PPP1R15A, and PMAIP1  in microarray  analyses of TRAIL  treated HMT-3522 S-1 MECs plated as monolayers  on a rigid rBM  (2D) or as spheroids  (3D) (mean ± SEM; n=3 independent experiments). ATF3, ***P = 0.0006; STC2, *P = 0.0353; PPP1R15A, *P = 0.0254; PMAIP1, *P = 0.0127 (Student's t test)."
NCBI gene IDs:
"ATF3: 467
PMAIP1: 5366
PPP1R15A: 23645
STC2: 8614"
Figure legend: "B Endogenous USP45 was immunoprecipitated (IP) from wild‐type (WT) and USP45 knockout (KO) KBM7 cells lines. The immunoprecipitates were resolved on a polyacrylamide gel and stained with Instant Blue. The gel was divided into the indicated sections, and proteins identified by mass spectrometry are detailed in Supplementary Table S1. The key proteins that we focus on in this study are indicated."
NCBI gene IDs:
"USP45: 85015
USP45: Q70EL2///85015"
Figure legend: "C Yeast two‐hybrid (Y2H) assays were performed with a GAL4 DNA‐binding domain fusion and/or activation domain for each protein indicated in the table to detect interaction between these proteins. Cells grown on media lacking LEU, TRP and HIS (to select for bait and prey plasmids) or tested for lacZ reporter gene activity."
NCBI gene IDs:
"GAL4: 
lacZ: "
Figure legend: "D, E Endogenous SLX4 and ERCC1 were immunoprecipitated from extracts of WT or ERCC1 KO MEFs (D), or WT or SLX4 KO MEFs (E) and subjected to immunoblotting with the indicated antibodies."
NCBI gene IDs:
"SLX4: 52864"
Figure legend: "C, D HEK293 cells were transiently transfected with constructs expressing the indicated wild‐type or mutant GFP-USP45 [1-62] fragment (C) or GFP full‐length USP45 (D) or empty GFP (C and D) as a control and processed as described in (A). LICOR-Odyssey quantitation of results shown in (C) is shown in Supplementary Fig S4."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "C USP45 KO U2OS cells were transiently transfected with a construct encoding FLAG‐ERCC1. Thirty‐six hours post‐transfection cells were treated with lactacystin (5 μM) for 6 h and lysed with a buffer containing 0.1 M N‐ethylmaleimide. Flag‐ERCC1 was immunoprecipitated and treated in the absence or presence of 0.1 μg of recombinant USP45 wild‐type (WT), non‐ERCC1‐binding USP45 [Asp25Ala, Glu26Ala] (AA) or catalytically inactive USP45 [Cys199Ala] (CA) for 1 h. The reactions were terminated by addition of sample buffer and analysed by immunoblot and Coomassie staining. Similar results were obtained in at least two separate experiments."
NCBI gene IDs:
"ERCC1: 2067
USP45: 85015"
Figure legend: "A Clonogenic survival assays were carried out in wild‐type (WT), USP45 knockout (KO) or USP45 KO cells re‐expressing wild‐type USP45 (RESWT) or catalytically inactive USP45 [Cys199Ala] (RESCA). The cells were treated with the indicated doses of mitomycin C (MMC) 16 h. Medium was changed and number of colonies quantified after 10 days. Each data point is the average of 3 experiments undertaken with 6 replicates ± SD."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "C, D As above, except rescue experiments are undertaken using the non‐ERCC1‐binding USP45 [Asp25Ala, Glu26Ala] mutant."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "A Wild‐type (WT), USP45 knockout (KO) or USP45 KO cells re‐expressing wild‐type USP45 (RESWT) or catalytically inactive USP45 [Cys199Ala] (RESCA) were used to analyse ERCC1 foci formation. Staining and analysis of endogenous ERCC1 foci formation was undertaken before (left panel), or after DNA damage induction by mitomycin C (40 ng/ml, 16 h, middle panel) or UV (20 J/m2 followed by 3‐h recovery, right panel). Three independent experiments were performed in which 500 cells per experiment were analysed. Scale bar, 10 μm.B Proportion of cells displaying more than 10 endogenous ERCC1 foci were quantified. Three independent experiments were performed in which 500 cells per experiment were analysed. Results are the mean of 3 experiments ± SD.C The wild‐type and USP45 knockout U2OS cells were treated with mitomycin C (40 ng/ml, 16 h) and total number of ERCC1 staining foci per cell was quantified in at least 50 independent cells at the indicated times. Similar results were obtained in two separate experiments. The data are presented as the average number of ERCC1 staining foci per cell."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "A Representative immunofluorescence microscopy images of endogenous wild‐type (WT) and USP45 knockout (KO) U2OS cells treated with no drug, BrdU (10 μM, 24 h), psoralen or angelicin (25 μM, 3 h). Cells were fixed 10 min after laser micro‐irradiation and stained for localisation of endogenous USP45, phosphorylated γ‐H2AX and DAPI. The right‐hand panel shows magnification of one micro‐irradiated cell. Similar results were obtained in at least three independent experiments. Scale bar, 10 μm."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "A U2OS cells were transiently transfected with the GFP-USP45 or GFP alone. Thirty‐six hours post‐transfection cells were irradiated with UV‐C (150 J/m2) through a micropore filter (lower panels) or left untreated (upper panels). Five minutes post‐irradiation, cells were fixed and co‐immunostained with antibodies recognising cyclobutane pyrimidine dimer (CPD) DNA lesions and GFP. The white arrows indicate co‐localisation between GFP-USP45 and CPD. Scale bar, 10 μm."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "B U2OS wild‐type (WT) and USP45 knockout (KO) cells were irradiated with UV‐C (20 J/m2). At the indicated post‐irradiation times, genomic DNA was extracted and subjected to Southern dot blot analysis using antibodies recognising CPD and dsDNA total antibody. Data are shown from a triplicate experiment in which each blot is derived from independent cells."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "A Live‐cell fluorescence microscopy analysis of U2OS cells stably expressing either GFPNLS empty, wild‐type (WT) USP45 or catalytically inactive USP45 [Cys199Ala]. Cells were treated with BrdU (10 μM, 24 h), psoralen or angelicin (25 μM, 3 h) and images captured at the indicated times after laser micro‐irradiation. The location of the micro‐irradiation stripe in each cell is indicated with a yellow line. Similar results were obtained in at least two independent experiments. Scale bar, 10 μm."
NCBI gene IDs:
"USP45: 85015"
Figure legend: "(D) Mean current responses to rapid switching from 140 mM CholGluc to 140 mM KGluc of HEK293T cells expressing WT EAAT1 (n=3) or WT EAAT2 (n=3). Cells were intracellularly dialyzed with a 115mM KGluc-based solution and held at 0 mV."
NCBI gene IDs:
"EAAT2: 6506
EAAT1: 6507"
Figure legend: "(D) Representative whole-cell current recordings from cells internally dialyzed with NaNO3 and glutamate in choline-NO3-based external solutions or in NaNO3-based solutions supplemented with 1 mM L-glutamate. Glutamate-induced changes in anion current amplitudes indicate that D399N and D486N EAAT2 (GltPh D312 and D405) are K+-independent, but are functionally expressed in the plasma membrane."
NCBI gene IDs:
"EAAT2: 6506"
Figure legend: "(E) Representative transport current recordings from cells expressing L448A or L448T (GltPh A360) or WT EAAT1 upon voltage jumps to ˗140 mV before and after superfusion with 1 mM l-glutamate. Cells were intracellularly dialyzed with a K+-free solution without permeating anions to isolate K+-independent transport currents. Transient capacitive currents during the first 5 ms after a voltage jump were blanked."
NCBI gene IDs:
"EAAT1: 6507"
Figure legend: "(F) Current-voltage relationship for net transport current amplitudes for WT and L448A/T EAAT1 (GltPh A360) for choline-based pipette solutions. Values are given as mean ± SD with indicated numbers of experiments."
NCBI gene IDs:
"EAAT1: 6507"
Figure legend: "(G) Time-dependent changes in L448A EAAT1 (GltPh A360) uptake currents during two repetitive glutamate applications and subsequent removals. Current amplitudes were measured at the end of voltage jumps to -140mV and plotted versus the time. Glutamate application is indicated by green bars. Values are given as mean ± SD of the last 100 data points in the current trace."
NCBI gene IDs:
"EAAT1: 6507"
Figure legend: "(H) Net transport current amplitudes for WT and L448A/T EAAT1 (GltPh A360) for different pipette solutions, Values are given as mean ± SD with indicated numbers of experiments (***, p&lt;0.001 Student's t-test)."
NCBI gene IDs:
"EAAT1: 6507"
Figure legend: "(E) Representative transport current recordings from a cell expressing R478A EAAT2 (GltPh R397) in the absence of internal K+. Transport currents were elicited by voltage jumps to -140mV before and after superfusion with 5 mM l-serine. Transient capacitive currents during the first 5 ms after a voltage jump were blanked."
NCBI gene IDs:
"EAAT2: 6506"
Figure legend: "(F) Time-dependent changes in steady-state R478A EAAT2 (GltPh R397) uptake currents during two consecutive serine applications and subsequent removals. Current amplitudes were measured at the end of voltage jumps to -140mV and plotted versus time. Serine application is indicated by green bars. Values are given as mean ± SD of the last 100 data points in the current trace."
NCBI gene IDs:
"EAAT2: 6506"
Figure legend: "(G) Current-voltage relationship for mean net transport currents for R478A EAAT2 induced by 5 mM l-serine for different intracellular solutions. Values are given as mean ± SD for the indicated number of experiments."
NCBI gene IDs:
"EAAT2: 6506"
Figure legend: "(H) Mean net transport currents for WT and R478A EAAT2 (GltPh R397) induced by 5 mM l-serine for different intracellular solutions. Values are given as mean ± SD for the indicated number of experiments."
NCBI gene IDs:
"EAAT2: 6506"
Figure legend: "High-resolution imaging of mitochondria in live cells using the Airyscan module of Zeiss LSM880 confocal microscope. H1975 cells transduced with matrix-targeted-DsRed and stained with NAO. Matrix-targeted Ds-Red differentiates matrix from cristae stained with NAO. Arrowheads point to cristae. Scale bar = 500 nm. N = 1 independent experiment."
NCBI gene IDs:
"DsRed: "
Figure legend: "High-resolution imaging of mitochondria in live cells using the Airyscan module of Zeiss LSM880 confocal microscope. The structure of the IMM in Mic13-KO cells (HeLa), stained with NAO. The number of cristae is decreased in Mic13-KO compared to control cells shown in panel A, labeled with arrowheads. Scale bar = 500 nm. N = 3 independent experiments."
NCBI gene IDs:
"Mic13: 125988"
Figure legend: "High-resolution imaging of mitochondria in live cells using the Airyscan module of Zeiss LSM880 confocal microscope. Live-cell imaging of the IMM in control and PTPMT1 KD H1975 cells, a model of cardiolipin deficiency. A gallery of mitochondria from various control H1975 cells expressing scrambled shRNA and stained with NAO, showing cristae (arrowheads). Scale bars = 500 nm. N = 3 independent experiments. A gallery of mitochondria from various H1975 cells expressing PTPMT1 shRNA and stained with NAO. Note the derangement of the ultrastructure (arrowheads). Scale bars = 500 nm. N = 3 independent experiments."
NCBI gene IDs:
"PTPMT1: 114971"
Figure legend: "The role of cristae structure as well as CJ formation and sealing on the generation of the difference in ΔΨm between cristae and IBM were studied Representative images of mitochondria in control (top row) vs. Mic13-KO (bottom row) HeLa cells. Note that TMRE FI in Mic13-KO mitochondria is distributed more evenly along the IMM, so that ΔΨCr-IBM is decreased. Scale bar = 500 nm. N = 3 independent experiments. Quantification of A, showing deletion of Mic13 in HeLa cells results in significant decrease in ΔΨCr-IBM. While Mic13-KO cells show a substantial decrease in cristae number, the loss of cristae does not appear absolute, making it feasible to compare TMRE FI at cristae relative to IBM. N = 3 independent experiments."
NCBI gene IDs:
"Mic13: 125988"
Figure legend: "The role of cristae structure as well as CJ formation and sealing on the generation of the difference in ΔΨm between cristae and IBM were studied by disrupting cristae using Mic10- and Mic60-deficient cells. Mic60 support cristae formation. Mic10 is essential for CJ formation, and, in its absence, cristae tend to remain as vesicles detached from IBM. Live-cell Airyscan imaging of TMRE was used in all figure panels and models. Representative images of Hap1 control (top row) vs. Mic60 KO (middle row) and Mic10 KO (bottom row); Mic60-KO mitochondria show very few cristae structures. TMRE staining along the IMM is relatively homogeneous, indicating decreased ΔΨCr-IBM. In general, Mic10 KO in Hap1 cells results in decreased TMRE signal intensity at cristae relative to IBM (arrowheads) as compared to control cells. Deletion of Mic10 induces detachment of cristae from IBM (see Figure 8). Except for the detached cristae vesicles, the TMRE staining is homogeneously distributed n Mic10-KO cells, indicating decreased ΔΨCr-IBM. Scale bar = 500 nm. N = 3 independent experiments. Quantification of C, showing Mic60 KO in Hap1 cells results in significant decrease in ΔΨCr-IBM. While Mic60-KO cells show a marked loss of normal cristae structures, depletion of cristae does not appear absolute, making it possible to compare TMRE FI at cristae relative to IBM. N = 3 independent experiments. Quantification of C, showing Mic10 KO in Hap1 cells results in a significant decrease in ΔΨCr-IBM. Note: quantification of ΔΨm at cristae relative to IBM was performed only on cristae structures that appeared to maintain attachment to IBM. N = 3 independent experiments."
NCBI gene IDs:
"Mic60: 10989
Mic10: 440574"