EMBO/SourceData huggingface dataset:
Figure legend: "F.  Co-immunoprecipitation assays showing binding  of BRCA2  and DPP9-SWT. HEK293 DPP9  KO + DPP9WT cells, were treated with 1µg/mL Dox  (24 h) to induce the expression of DPP9-FLAG. DNA damage was induced with 300 nM MMC  treatment for 24h. Control cells do not express DPP9  (- Dox). Bound proteins were eluted with a FLAG  peptide and analysed by Western Blotting (anti-BRCA2: RRID:AB_2259370, anti-DPP9: RRID:AB_731947, anti-FLAG  RRID:AB_262044)."
NCBI gene IDs:
"FLAG: 
DPP9: 91039"
Figure legend: "B.  Pull-down  assay showing direct binding  of purified recombinant DPP9  to a BRCA2  N-terminal1-40 fragment immobilized on HA-beads. The DPP9  inhibitors 1G244  or SLRFLYEG  compete with BRCA21-40HA for interaction  with DPP9. Representative data of three technical replicates is shown. Anti-HA: RRID:AB_2565334, anti-DPP9: RRID:AB_2889071."
NCBI gene IDs:
"HA: 
BRCA2: 675"
Figure legend: "A-B. Representative  Western Blots  and accompanying graph from more than three biological replicates show that MMC  (300 nM, 24 h) induces a rapid turnover  of endogenous BRCA2  in HeLa  WT cells, which is less pronounced in HeLa  WT cells treated with the proteasome  inhibitor MG132  (100µM), in HeLa DPP9  KD cells and in cells transiently silenced for DPP9  (siDPP9). RAD51  stability is not altered in HeLa DPP9  KD cells. Vinculin  is a loading control for BRCA2, Tubulin  is a loading control for RAD51. Shown images originate from one representative cycloheximide  (CHX) chase assay. The ratios of BRCA2  to Vinculin  and RAD51 to Tubulin  are defined as 100% at time  0 h. Mean ± SEM, data were analysed by a paired two-tailed t-test. (*= p ≤ 0.03). Anti-BRCA2: RRID:AB_2259370, anti-DPP9: RRID:AB_731947, anti-Vinculin: RRID:AB_477629, anti-Tubulin: RRID:AB_628412, anti-RAD51: RRID:AB_1142428."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "C-D. Representative  Western Blots and graph summarizing results of more than three biological replicates. CHX  chase assays show that the MMC-induced degradation   of BRCA2  is less pronounced in HEK293 DPP9  KO cells as well as in HEK293 DPP9  KO + DPP9-SS729A cells overexpressing an inactive DPP9-S mutant. Expression of DPP9  was induced (+ Dox, 1µg/ml) simultaneously with MMC  (300 nM), 24 h. Cells that were overexpressing the active variant (DPP9-SWT) show similar levels of MMC-induced BRCA2 degradation. RAD51 stability  is not altered by MMC  and is similar in all cell lines. Vinculin  is a loading control for BRCA2, Tubulin  is a loading control for RAD51. Shown images originate from one representative cycloheximide  (CHX) chase assay. The ratios of BRCA2  to Vinculin  are defined as 100% at time  0 h. Mean ± SEM, data were analysed by a paired two-tailed t-test (*= p ≤ 0.03, **= p ≤ 0.002). Antibodies as described in (A-B)."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "F-G. Representative Western Blots  and graphs summarizing CHX  assays from three biological replicates showing that the BRCA2∆MP3-1000 truncation mutant is degraded at a higher rate compared to the untruncated BRCA21-1000. Cells were co-transfected with a GFP  expressing plasmid, as transfection and loading control. Control cells were transfected with GFP  only (U.T). Image originates from one representative experiment. BRCA2-FLAG  signals are related to the transfection and loading control GFP. BRCA2  levels in relation to GFP  were defined as 100 % at time  0 h. Mean ± SEM, data were analysed by a paired two-tailed t-test (*= p ≤ 0.03). Anti-FLAG: RRID:AB_262044, anti-GFP: RRID:AB_641123."
NCBI gene IDs:
"GFP: 
BRCA2: 675"
Figure legend: "A. Representative immunofluorescence  images of γH2AX  signals (white, RRID:AB_309864) in gki MEF DPP9S729A, showing more γH2AX  in gki MEF DPP9S729A cells following removal of Neocarzinostatin  (NCS), quantification in (B). MEF DPP9S729A and control MEF  cells were treated with 250 ng/mL NCS  for 30 min and allowed to recover for the indicated time-points. γH2AX  signals at time  0, reflect 30 min of NCS  and no recovery time. Nuclei  are shown in blue (DAPI). Scale bar 10 µm. B. Quantification of γH2AX  in gki MEF DPP9S729A and control MEF  cells as described in (A). Signals from more than 1700 cells were quantified per condition per experiment. Mean ± SEM from six biological replicates, each in technical duplicates. Data were analysed by an unpaired two-way ANOVA with Sidak's multiple comparison test (* = p ≤ 0.05, ** = p ≤ 0.01)."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "C. Representative PLA  images of BRCA2-PALB2 PLA  experiments showing more PLA  events (white) in HeLa  cells silenced for DPP9  (siDPP9) with respect to cells treated with non-targeting siRNA (siNT). Phalloidin  (green) stains actin filaments, DAPI  (blue) stains  the nucleus. Scale bar 10 µm. Anti-PALB2: RRID:AB_890607, anti-BRCA2: RRID:AB_2259370."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "D. Quantification of PLAs  experiments showing more BRCA2-PALB2 PLA events in cells silenced for DPP9  (siDPP9) in comparison to non-targeting controls (siNT). Each dot represents the number of PLA  events in a single cell, from four biological replicates. The technical control samples (NgtCntrl) omitted the BRCA2  antibody. The number of foci is shown based on their cellular localisation. Data were analysed by a two-way ANOVA, with Tukey's Multiple Comparison test. Shown are Mean ± SEM (**** = p ≤ 0.0001)."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "E. Representative PLA  images of γH2Ax-BRCA2 PLA  experiment showing a reduction in the number of PLA  events (white) between γH2Ax  and BRCA2  in HeLa DPP9  KD cells. Phalloidin  (green) stains actin filaments, DAPI  (blue) stains  the nucleus. Scale bar 10 µm. Anti-γH2Ax: RRID:AB_2118009, anti-BRCA2: RRID:AB_2259370 F. Quantification of PLAs  showing fewer MMC-induced γH2Ax-BRCA2 PLA  events in HeLa DPP9  KD cells, in comparison to HeLa  WT cells. Each dot represents the number of PLA  events in a single cell, from three biological replicates. The technical control samples (NgtCntrl) omitted the γH2Ax  antibody. Data were analysed by a two-way ANOVA, with Tukey's Multiple Comparison test. Shown are Mean ± SEM (**** = p ≤ 0.0001)."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "A. Representative immunofluorescence  images showing that re-expression of DPP9-SWT leads to an increase in the number of RAD51  foci formed following exposure to MMC  in HEK293 DPP9  KO + DPP9-SWT cells. Expression of DPP9  was induced (+ Dox, 1µg/ml) simultaneously with MMC  (300 nM), 24 h. RAD51  foci are shown in white, nuclei  (DAPI) are shown in blue. Scale bar 10 µm. Anti-RAD51: RRID:AB_1142428. B. Graph showing the number of RAD51  foci following induction of DPP9-SWT expression, compared to uninduced HEK293 DPP9  KO + DPP9WT  cells (-Dox). Induction of HEK293 DPP9  KO + DPP9S729A for expression of DPP9-SS729A did not result in more RAD51  foci. Each dot represents the number of RAD51  foci in a single cell, from three biological replicates. Data were analysed by a two-way ANOVA, with Tukey's Multiple Comparison test. Shown are Mean ± SEM (**** = p ≤ 0.0001)."
NCBI gene IDs:
"DPP9: 91039"
Figure legend: "Representative images (C, and summarizing graph (D showing the number of RAD51  foci per nucleus  in HeLa  WT cells (C, D) Where stated, cells were treated with control siRNA (siNT) or silenced for BRCA2, and transiently transfected with the BRCA21‑3418 or BRCA23-3418 constructs. Both BRCA2  constructs can rescue the RAD51  foci formation phenotype to the control levels in HeLa  WT cells (C, D). Each dot represents the number of RAD51  foci in a single cell, from three (D) biological replicates. Data were analysed by a two-way ANOVA, with Tukey's Multiple Comparison test. Shown are Mean ± SEM (**** = p ≤ 0.0001). RAD51  foci are shown in white, nuclei  (DAPI) are shown in blue. Scale bar 10 µm. Anti-RAD51: RRID:AB_1142428; ; anti-BRCA2: RRID:AB_2259370."
NCBI gene IDs:
"BRCA2: 675"
Figure legend: "Representative images , E) and summarizing graph F) showing the number of RAD51  foci per nucleus  in HeLa DPP9  KD cells (E, F). In HeLa DPP9  KD cells significantly more RAD51  foci were present in cells transfected with BRCA2∆MP3-3418 compared to BRCA2  silenced cells and cells expressing the untruncated BRCA21-3418 (E, F). Each dot represents the number of RAD51  foci in a single cell, from four (F) biological replicates. Data were analysed by a two-way ANOVA, with Tukey's Multiple Comparison test. Shown are Mean ± SEM (**** = p ≤ 0.0001). RAD51  foci are shown in white, nuclei  (DAPI) are shown in blue. Scale bar 10 µm. Anti-RAD51: RRID:AB_1142428; anti-BRCA2: RRID:AB_2259370."
NCBI gene IDs:
"BRCA2: 675
DPP9: 91039"
Figure legend: "(I) Representative images showing the phospho-AMPK immunolabeling (Pseudo-colored) in low-density cortical neurons on the basal condition and on DHPG treatment for 5 minutes in the presence of scrambled siRNA and in the presence of α-SNAP siRNA. MAP2B immunolabeling was used for identifying neurons and intensity was used for normalization. Scale bar 10μm."
NCBI gene IDs:
"α-SNAP: 140673"
Figure legend: "(J) Box plot showing the normalized phospho-AMPK intensity distribution across multiple neurons on the basal condition in the presence of scrambled siRNA, on DHPG treatment for 5 minutes in the presence of scrambled siRNA, on the basal condition in the presence of α-SNAP siRNA, on DHPG treatment for 5 minutes in the presence of α-SNAP siRNA. Data points in all groups were normalized to the average of the scrambled siRNA basal group. The box extends from 25th to 75th percentile with the middlemost line representing the median of the dataset. Whiskers range from minimum to maximum data point. *p&lt;0.05, n=29-35 cells per group from 3 independent platings."
NCBI gene IDs:
"α-SNAP: 140673"
Figure legend: "A Expression patterns of pSEU::SEU-GFP and pWOX5::GFP in the embryos of the indicated genotypes at dermatogen, early globular, and heart stages. White arrows indicate the QC precursor cell, and white dashed lines indicate embryos. Scale bars: 10 µm."
NCBI gene IDs:
"GFP: 
SEU: 840981
WOX5: 820297"
Figure legend: "B Quantification of pWOX5::GFP GFP fluorescence in wild-type (WT) and seu-3 mutant embryos. Fluorescence intensity at the early globular stage of WT embryos was set to 1. Data Information: In (B) data represent mean ± SD of three independent replicates. n denotes the total number of scored samples. Individual values (black dots) are shown. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"GFP: 
seu: 840981
WOX5: 820297"
Figure legend: "C, D Expression pattern of pWOX5::GFP in WT (C) and seu-3 (D) embryos at 5 days after germination (DAG). Scale bars: 20 µm. E Quantification of GFP fluorescence in the QC of pWOX5::GFP transgenic plants, as shown in (C) and (D). Fluorescence intensity was normalized to the WT. Data Information: (E), data represent mean ± SD of three independent replicates. n denotes the total number of scored samples. Individual values (black dots) are shown. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"GFP: 
seu: 840981
WOX5: 820297"
Figure legend: "F RT-qPCR analysis of the relative expression levels of WOX5 in WT and seu-3 roots. Total RNA was extracted from 5 mm root tip sections of seedlings at 5 DAG. Data Information: (F) data represent mean ± SD of three independent replicates. n denotes the total number of scored samples. Individual values (black dots) are shown. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"seu: 840981
WOX5: 820297"
Figure legend: "L, M Double staining of the QC184 β-glucuronidase (GUS) marker (light blue) and starch granules (dark brown) in WT (L) and seu-3 (M) seedlings at 5 DAG. In white arrows indicate the CSCs. Scale bars: 20 μm."
NCBI gene IDs:
"seu: 840981"
Figure legend: "N, O Expression pattern of J2341 in WT (N) and seu-3 (O) seedlings at 5 DAG. P, Q Expression pattern of CS9227 in WT (P) and seu-3 (Q) seedlings at 5 DAG. In white arrows indicate the CSCs. Scale bars: 20 μm."
NCBI gene IDs:
"seu: 840981"
Figure legend: "A-C Expression pattern of pWOX5::GFP in WT (A), scr-3 (B) and plt1-4 plt2-2 (C) at 5 DAG. D Quantification of GFP fluorescence in the QC of pWOX5::GFP transgenic seedlings, as shown in (A-C). GFP signal intensity in each genotype was normalized relative to that in the WT. Data information: In (D) n denotes the total number of scored samples. Individual values (black dots) are shown. Data represent mean ± SD of three independent replicates. In (D) different lowercase letters indicate significant differences by one-way ANOVA followed by Tukey's multiple comparison test (P &lt; 0.01)."
NCBI gene IDs:
"GFP: 
plt1: 821632
plt2: 841542
scr: 824589
WOX5: 820297"
Figure legend: "E RT-qPCR analysis of the relative expression levels of WOX5 in WT, scr-3, and plt1-4 plt2-2 roots. Total RNA was extracted from 5 mm root tip sections of seedlings at 5 DAG. Data information: In (E) , **P &lt; 0.01 (Student's t-test). Scale bars: 20 µm."
NCBI gene IDs:
"plt1: 821632
plt2: 841542
scr: 824589
WOX5: 820297"
Figure legend: "K Quantification of GFP fluorescence in the QC of pSCR::GFP and pSCR::GFP-SCR transgenic seedlings, as shown GFP signal intensity of each genotype was normalized relative to that of the WT. Data information (K), n denotes the total number of scored samples. Individual values (black dots) are shown. Data represent mean ± SD of three independent replicates. (K), **P &lt; 0.01 (Student's t-test). Scale bars: 20 µm."
NCBI gene IDs:
"GFP: 
SCR: 824589"
Figure legend: "A Yeast two-hybrid (Y2H) assays showing the interaction between SEU and SCR. The yeast transformants were plated on synthetic defined (SD) media lacking Leu and Trp (SD/-2) or lacking Ade, His, Leu, and Trp (SD/-4) to assess protein-protein interactions. AD, GAL4 activation domain; BD, GAL4 DNA-binding domain."
NCBI gene IDs:
"GAL4: 855828"
Figure legend: "C Verification of in vivo interactions between SCR and SEU in N. benthamiana leaves via Co-IP assays. SCR-GFP and SEU-myc were transiently coexpressed in N. benthamiana leaves. Protein samples were immunoprecipitated using anti-myc antibody."
NCBI gene IDs:
"GFP: 
myc: 
SCR: 824589
SEU: 840981"
Figure legend: "D Co-IP assays of SEU with SCR in Arabidopsis. Protein extracts from WT and SCR-GFP roots were isolated at 5 DAG and immunoprecipitated with anti-GFP antibody."
NCBI gene IDs:
"GFP: 
SCR: 824589"
Figure legend: "F, G SCR and SEU physically bind to the WOX5 promoter, as shown by ChIP-qPCR analysis. Chromatin was isolated from 5 mm root tip sections of seedlings at 5 DAG, sonicated, and immunoprecipitated using anti-GFP antibody. The precipitated DNA was used as a template for qPCR analysis. A, B, and I indicated the PCR amplicons ACT7, control. Data information: In (F), (G) data represent mean ± SD of three independent replicates. **P &lt; 0.01 (Student's t-test)."
NCBI gene IDs:
"ACT7: 830841
WOX5: 820297"
Figure legend: "H Mutation of the SCR gene impairs the recruitment of SEU to the WOX5 promoter, as shown by ChIP-qPCR analysis. Chromatin was extracted from SEU-GFP and SEU-GFP scr-3 seedlings at 5 DAG and precipitated with anti-GFP antibody. ChIP signals were quantified by qPCR as a percentage of total input DNA. Data information: In (H) data represent mean ± SD of three independent replicates. **P &lt; 0.01 (Student's t-test)."
NCBI gene IDs:
"GFP: 
SCR: 824589
scr: 824589
SEU: 840981
WOX5: 820297"
Figure legend: "I SEU stimulated SCR-mediated WOX5 promoter activation in transient expression assays in N. benthamiana leaves. The pWOX5::LUC reporter was cotransformed with the indicated effector constructs. The pWOX5::LUC activity was normalized relative to the internal control (LUC/Renilla luciferase [REN]). The schematic diagram shows the construct used in the transient expression assays. Arrows indicate promoter regions, and boxes indicate coding sequences. Different lowercase letters indicate significant differences by one-way ANOVA followed by Tukey's multiple comparison test (P &lt; 0.01). Data information: In (I) data represent mean ± SD of three independent replicates. **P &lt; 0.01 (Student's t-test)."
NCBI gene IDs:
"LUC: 
luciferase: 
SCR: 824589
SEU: 840981
WOX5: 820297"
Figure legend: "J-M Representative confocal images of the indicated genotypes at 5 DAG. Scale bars: 20 μm. Insets show the QC region in which the solid white lines indicate the QC of WT, and the dashed white lines indicate the QC of seu-3. (Insets scale bars: 5 μm). N Quantification of GFP signal intensity in the QC of WT and seu-3 seedlings expressing pWOX5::SEU-GFP and pWOX5::SCR-GFP. GFP signal intensity in seu-3 seedlings was normalized relative to the WT. Individual values (black dots) are shown. n denotes the total number of scored samples. Data information: In (N), data represent mean ± SD of three independent replicates. **P &lt; 0.01 (Student's t-test)."
NCBI gene IDs:
"GFP: 
SCR: 824589
seu: 840981
SEU: 840981
WOX5: 820297"
Figure legend: "C SDG4 physically bind to the WOX5 promoter, as shown by ChIP-qPCR analysis. ACT7, control. Data information Chromatin was isolated from 5 mm root tip sections of seedlings at 5 DAG, sonicated, and immunoprecipitated using anti-GFP or anti-H3K4me3 antibodies. The precipitated DNA was used as a template for qPCR analysis. In (C) data represent mean ± SD of three independent replicates. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"ACT7: 830841
WOX5: 820297"
Figure legend: "D, E ChIP-qPCR analysis indicating that mutations in SDG4 and SEU genes impair H3K4me3 deposition in the WOX5 promoter. ChIP signal was normalized to the WT. Individual values (black dots) are shown. Data information: Chromatin was isolated from 5 mm root tip sections of seedlings at 5 DAG, sonicated, and immunoprecipitated using anti-GFP or anti-H3K4me3 antibodies. The precipitated DNA was used as a template for qPCR analysis. In (D), (E) data represent mean ± SD of three independent replicates. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"SDG4: 829210
SEU: 840981
WOX5: 820297"
Figure legend: "F, G Expression pattern of pWOX5::GFP in the indicated genotypes at 5 DAG. Scale bars: 20 µm. H Quantification of GFP fluorescence in the indicated genotypes expressing pWOX5::GFP. GFP signal intensity in the WT was set to 1. n denotes the total number of scored samples. Data information: In (H) data represent mean ± SD of three independent replicates. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"GFP: 
WOX5: 820297"
Figure legend: "I RT-qPCR analysis of the relative expression levels of WOX5 in WT and ashr3-1 roots. Total RNA was extracted from 5 mm root tip sections of seedlings at 5 DAG. Individual values (black dots) are shown. Data information: In (I) data represent mean ± SD of three independent replicates. **P &lt; 0.01, *P &lt; 0.05 (Student's t-test)."
NCBI gene IDs:
"ashr3: 829210
WOX5: 820297"
Figure legend: "I SEU associates with SCR and SDG4 in N. benthamiana leaves, as shown by Co-IP assays. SDG4-myc, SCR-HA, and SEU-GFP were coinfiltrated into N. benthamiana leaves. Protein samples were immunoprecipitated with anti-GFP antibody and immunoblotted with anti-myc and anti-HA antibody. N. benthamiana leaves coinfiltrated with SDG4-myc and SCR-HA were used as a negative control."
NCBI gene IDs:
"HA: 
myc: 
GFP: 
SCR: 824589
SDG4: 829210
SEU: 840981"
Figure legend: "J Co-IP assays of SCR with SEU and SDG4 in Arabidopsis. Proteins extracted from SDG4-myc and SDG4-myc SCR-GFP plants were immunoprecipitated using anti-GFP antibody, and immunoblotted using anti-myc and anti-SEU antibodies."
NCBI gene IDs:
"GFP: 
myc: 
SCR: 824589
SDG4: 829210"
Figure legend: "D. The morphology of oocytes and embryos from female patients with MOS variants. The day of oocyte retrieval was defined as day 0. Polar bodies were indicated by black arrows. Scale bar=20 µm."
NCBI gene IDs:
"MOS: 4342"
Figure legend: "B. The immunofluorescence images show the pERK1/2 (red) dynamics in immature (GV, n=5), mature (MII, n=10), and fertilized oocytes from unidentified control patients (n=6), as well as in mature oocytes from patient 1 carrying homozygous MOSAsn95Lys variants. pERK1/2 activation was inhibited after U0126 treatment (n=6). FITC-α-tubulin (Green) and DAPI (blue) were used for co-staining. Scale bar=10 µm."
NCBI gene IDs:
"MOS: 4342"
Figure legend: "C. Western blot analysis of the MEK1/2 and ERK1/2 activation after transfection of FLAG-tagged wild-type MOS and identified MOS variants in HEK 293 cells."
NCBI gene IDs:
"FLAG: 
MOS: 4342"
Figure legend: "F. Immunofluorescence of FLAG (green) and pERK1/2 (grey) in oocytes after injection of different MOS variant mRNAs combined with mCherry mRNAs. The signal of mCherry was directly captured after fixation, with DAPI staining for DNA visualization (blue). (n=over 30 oocytes in each group). Scale bar=10 µm."
NCBI gene IDs:
"mCherry: 
MOS: 4342"
Figure legend: "A. Immunofluorescence of FLAG (red) and F-actin (gray) in oocytes after microinjection of negative control or mouse Mos siRNAs combined with or without wild-type human MOS or MOS variants mRNAs and culture in medium with 2.5 µM milrinone for 24 h, followed by release to maturation (n=15-20 oocytes each group). Scale bar= 10 µm."
NCBI gene IDs:
"Mos: 17451
MOS: 4342"
Figure legend: "C. The immunofluorescence of F-actin (red) and TPX2 (green) in WT and Erk1/2oo-/- oocytes, using DAPI (blue) co-staining (n=15-20 oocytes for each group). The zoomed images of F-actin are displayed in gray. Scale bar=10 µm. D. The box plot summarizing the relative intensities of F-actin between control and ERK1/2oo-/- oocytes (n=7-10 oocytes for each group)."
NCBI gene IDs:
"Erk1: 26417
ERK1: 26417"
Figure legend: "E. Representative bright field images showing the morphology of oocytes and embryos from wildtype and ERK1/2oo-/- mice. The MII oocyte, zygote, 2-cell and blastocyst embryos were harvested in vivo at 14, 24, 48, and 96 h post-hCG, respectively (n=3 mice for each timepoints in two groups). Scale bar=100 µm. F, G. Bar graphs shows the blastocyst percentage (F) and fragment percentage (G) in wild-type (n=51) and maternal ERK1/2 deletion embryos (n=63)."
NCBI gene IDs:
"ERK1: 26417"
Figure legend: "H. The bar graphs show the expression levels of selected MOS-regulated decay genes (n=2 or 3 biological replicates)."
NCBI gene IDs:
"MOS: 4342"
Figure legend: "H. Heatmap showing the expression level of selected maternal genes, mitochondrial translation genes and mitochondrial respiratory chain genes regulated by MOS mutation or ERK1/2 inactivation."
NCBI gene IDs:
"ERK1: 5595
MOS: 4342"
Figure legend: "E. Representative images displaying JC-1 monomers (green), aggregates (red) and bright filed images of GV and MII oocytes from wild-type (WT) and Erk1/2oo-/- oocytes (n=3 mice in each group). Erk1/2oo-/- oocytes exhibited big and multiple polar bodies. Scale bar=100 µm."
NCBI gene IDs:
"Erk1: 26417"
Figure legend: "F. Confocal images of JC-1 monomers (green) and aggregates (red) of GV and MII oocytes from wild-type (WT) and Erk1/2oo-/- oocytes (n=3 mice in each group). Erk1/2oo-/- oocytes exhibited big and multiple polar bodies, indicated by red arrows. Hoechst 33342 (blue) was co-stained to represent nucleus or chromosome. Scale bar=10 µm."
NCBI gene IDs:
"Erk1: 26417"
Figure legend: "(D) PY79 (WT) and ET19 (∆sigX) cells were infected with SPP1 at either high (phages:bacteria 1:1) or low (1:20) MOI, and OD600nm was followed at 2 min intervals. Shown is a representative experiment out of 6 biological repeats, and the average values and SD of 8 technical repeats."
NCBI gene IDs:
"sigX: 938966"
Figure legend: "(E) BDR2637 (Pveg-mCherry) (WT, purple) and ET191 (PrrnE-gfp, ∆sigX) (∆sigX, cyan) cells were mixed, infected with low concentrations (10-8 PFU/ml) of SPP1, placed on an agarose pad and plaque formation was followed by time lapse confocal microscopy. Shown are overlay images from mCherry (purple) and GFP (cyan) signals of the bacterial lawn captured at the indicated time points (hrs). The plaque is seen as a hole formed on the bacterial lawn. Scale bar 100 µm. Corresponds to Movie EV4."
NCBI gene IDs:
"gfp: 
mCherry: 
rrnE: 8303206
sigX: 938966
veg: 936744"