EMBO/SourceData huggingface dataset:
Figure legend: "(A) Morphological phenotypes of transgenic plants over-expressing MEKK2-FLAG protein in WT and summ3-17 background. Line 1 and 2 express MEKK2-FLAG in WT background. Line 3-5 express MEKK2-FLAG in the summ3-17 mutant background. The photograph shows four-week-old soil grown plants. Expression of MEKK2-FLAG was detected by western blot using an anti-FLAG antibody."
NCBI gene IDs:
"summ3: 815625
MEKK2: 826407"
Figure legend: "(D-E) Expression levels of PR1 (D) and PR2 (E) in the indicated genotypes as determined by quantitative RT-PCR. Values were normalized to the expression levels of ACTIN1. The data are shown as mean ± SD (n=3) with one-way ANOVA analysis and Tukey test (P &lt; 0.01). Different letters indicate significant differences. The experiments were repeated three times with similar results."
NCBI gene IDs:
"ACTIN1: 
PR2: 824893
PR1: 815949"
Figure legend: "(A) Quantification of luciferase activity in N. benthamiana leaves expressing CRCK3NLuc and MPK4CLuc or MPK6CLuc. Data were collected three days after infiltration. The data are shown as mean ± SD (n=8) with one-way ANOVA analysis and Tukey test (P &lt; 0.01). Different letters indicate significant differences. The experiments were repeated twice with similar results."
NCBI gene IDs:
"CRCK3: 815625
MPK4: 828151
MPK6: 818982"
Figure legend: "(B) Phosphorylation of CRCK3G390R by MPK4. MPK4 was immunoprecipitated from wild type or MPK4::MPK4-FLAG. After incubation with [γ-32P] ATP and the immunoprecipitated MPK4-FLAG protein in protein kinase buffer, CRCK3G390R was separated by SDS-PAGE. The autoradiograph of the gel is shown in the top panel, and immunoblot analysis of MPK4-FLAG levels is shown in the bottom panel. Myelin basic protein (MBP) was used as a positive control. This experiment was repeated three times with similar results."
NCBI gene IDs:
"MPK4: 828151"
Figure legend: "(C) Western blot analysis of CRCK3-FLAG proteins in wild type and mpk4-3. Total protein was extracted from two-week-old CRCK3-FLAG transgenic plants in WT and mpk4 background grown on ½ MS medium plates. The protein extracts were treated with or without λ-PMPase phosphatase. The proteins were separated by Phos-tagTM acrylamide gel electrophoresis and CRCK-FLAG proteins were detected by western blot analysis using an anti-FLAG antibody. Similar results were obtained in two independent experiments. pCRCK3, phosphorylated CRCK3-FLAG protein."
NCBI gene IDs:
"mpk4: 828151"
Figure legend: "(D) Western blot analysis of CRCK3-FLAG and CRCK35S-5A-FLAG proteins in transgenic plants of wild type background. Total protein was extracted from two-week-old plants grown on ½ MS medium plates. The protein extracts were treated with or without λ-PMPase phosphatase. The proteins were separated by Phos-tagTM acrylamide gel electrophoresis and the FLAG-tagged proteins were detected by western blot analysis using an anti-FLAG antibody. Similar results were obtained in two independent experiments. 5S-5A stands for a change in five amino acids (S172A, S181A, S188A, S195A, S202A)."
NCBI gene IDs:
"CRCK3: 815625"
Figure legend: "(A) In vivo pull-down of HA-tagged SUMM2 by FLAG-tagged CRCK3.FLAG-tagged full-length or truncated CRCK3 and HA-tagged SUMM2 proteins were transiently expressed in N. bethamiana by agro-infiltration. FLAG-tagged CRCK3 proteins were immunoprecipitated with anti-FLAG conjugated beads (Sigma) and detected by Western blot using anti-FLAG antibody. HA-tagged SUMM2 protein co-immunoprecipitated with FLAG-tagged CRCK3 was detected by Western blot using anti-HA antibody. Similar results were obtained in two independent experiments."
NCBI gene IDs:
"CRCK3: 815625"
Figure legend: "(B) In vivo pull-down of HA-tagged SUMM2 by FLAG-tagged CRCK3 kinase domain. KD represents a truncation of SUMM3 with only the kinase domain (amino acid 201-510).FLAG-tagged full-length or truncated CRCK3 and HA-tagged SUMM2 proteins were transiently expressed in N. bethamiana by agro-infiltration. FLAG-tagged CRCK3 proteins were immunoprecipitated with anti-FLAG conjugated beads (Sigma) and detected by Western blot using anti-FLAG antibody. HA-tagged SUMM2 protein co-immunoprecipitated with FLAG-tagged CRCK3 was detected by Western blot using anti-HA antibody. Similar results were obtained in two independent experiments."
NCBI gene IDs:
"CRCK3: 815625"
Figure legend: "B, Complementation analysis of DNA synthesis. Stable cell lines expressing BRPF3 resistant to the BRPF3/a siRNA and control (lacZ-V5) were siRNA transfected and analysed for EdU incorporation as in A. Error-bars, SD; n = 6 biological replicas. Two-tailed t test, ** p&lt;10-2."
NCBI gene IDs:
"lacZ: 
BRPF3: 27154"
Figure legend: "D, Immunoprecipitation analysis of BRPF3 deletion mutants. One representative experiment out of two is shown."
NCBI gene IDs:
"BRPF3: 27154"
Figure legend: "E, Histone acetyltransferase assay (HAT) with purified BRPF1/3 complexes on free histones and mononucleosomes."
NCBI gene IDs:
"BRPF1: 7862"
Figure legend: "F, HAT assay with purified BRPF1/3 complexes on H3 peptides unmodified or acetylated (ac)/methylated (me) at K14 and K27, respectively."
NCBI gene IDs:
"BRPF1: 7862"
Figure legend: "G, Analysis of histone acetylation in BRPF3 depleted cells. (left) Western blot of siRNA treated U-2-OS cells. TSA treatment (1 hr) was included as a positive control. 2x, double amount of extract loaded in 1x. (right) H3K14 acetylation levels quantified relative to total H3. Error bars, SD; n = 3 biological replicates."
NCBI gene IDs:
"BRPF3: 27154"
Figure legend: "H, Complementation analysis of H3K14ac. Total cell extracts of siRNA treated lacZ-V5 and BRPF3-V5 (siBRPF3/a resistant) expressing cells were analysed by western blotting. One representative experiment out of four biological replicas is shown."
NCBI gene IDs:
"lacZ: 
BRPF3: 27154"
Figure legend: "G, Analysis of chromatin-bound proteins in HBO1 depleted cells as in E. One representative experiment out of two biological replicas is shown."
NCBI gene IDs:
"HBO1: 11143"
Figure legend: "A, ChIP-seq analysis of BRPF3 binding on the human genome. BRPF3 enrichment signal obtained by ChIP-seq with asynchronous (AS), synchronized (S) and released into HU (S+HU) RKO cells, on the MCM4 (left) and Lamin B2 (right) replication origins. Signals were compared to the published enrichment signals for ORC1 in HeLa cells ((Dellino et al, 2013), GSE37583), HBO1 in RKO cells (Avvakumov et al., 2012, GSE33221), and H3K14ac in IMR90 cells (GSM521881). The negative control IgG signal shown is from the S+HUchromatin."
NCBI gene IDs:
"H3: 
BRPF3: 27154
HBO1: 11143
Lamin B2: 84823
MCM4: 4173"
Figure legend: "B, Venn diagrams illustrating the overlap of genome binding sites between (top) BRPF3, HBO1 and ORC1, and (bottom) BRPF3, BRPF1/2 and ORC1 in human cells."
NCBI gene IDs:
"BRPF1: 7862
BRPF3: 27154
HBO1: 11143"
Figure legend: "C, Heatmaps of BRPF3, HBO1 and H3K14ac ChIP-seq signal on ± 5kb surrounding the TSS of genes. Genes were sorted by BRPF3 abundance from high to low. A.U. arbitrary unit."
NCBI gene IDs:
"H3: 
BRPF3: 27154
HBO1: 11143"
Figure legend: "D, Average profiles of BRPF3 and H3K14ac ChIP-seq signal on ± 2.5kb around the centre of (top) BRPF3 and (bottom) ORC1 genome binding sites."
NCBI gene IDs:
"H3: 
BRPF3: 27154"
Figure legend: "E, ChIP-qPCR analysis of H3K14ac on well-characterized replication origins. H3K14ac enrichments measured relative to IgG control are shown relative to control siRNA treated cells. Error bars, range; n= 2 biological replicas."
NCBI gene IDs:
"H3: "
Figure legend: "F, Expression levels of DNA replication factors were not altered by the lack of BRPF3. Each point represents the expression levels of a gene in control and BRPF3 depleted cells (GSE65065). Replication factors were classified as described previously (Alabert et al, 2014). Total genes (grey), DNA replication factors (colors) and BRPF3 (black) expression levels are shown. Continuous black lines mark 1.5 fold change in expression levels."
NCBI gene IDs:
"BRPF3: 27154"
Figure legend: "A, Western blot of DNA damage signalling in BRPF3 depleted cells. siRNA transfected U-2-OS cells were treated with HU for 2 h or 24 h. One representative experiment out of two biological experiments is shown."
NCBI gene IDs:
"BRPF3: 27154"
Figure legend: "(A) Assay of Tcf7l1 mRNA by RT-PCR for the first 4 days of differentiation in the presence or absence of 3 μM CH. Average and SD of three independent experiments. Day2 *p= 0.048, Day3 *p=0.036."
NCBI gene IDs:
"Tcf7l1: 21415"
Figure legend: "(C) Assay of FoxA2 mRNA by RT-PCR for the first 3 days of differentiation in the presence or absence of 3 µM CH. Average and SD of three independent experiments. **p=5x10-7."
NCBI gene IDs:
"FoxA2: 15376"
Figure legend: "(D) ChiP-seq data from a compendium of transcription factor ChIP-seq analyses (Sánchez-Castillo et al, 2015). Gene tracks represent binding of Tcf7l1 at the FoxA2, Nodal and Eomesodermin (Eomes) gene locus. Red lines indicate the regions analyzed by ChIP PCR for Tcf7l1."
NCBI gene IDs:
"Eomes: 13813
Eomesodermin: 13813
FoxA2: 15376
Tcf7l1: 21415"
Figure legend: "(E) ChIP for Tcf7l1 followed by qPCR for the regions indicated in Fig. 2D. Analysis was performed in wild-type ES cells in 2i culture conditions. Average and SD of three independent experiments. Klf2 *p=0.025, FoxA2 *p=0.050, Eomes *p=0.023."
NCBI gene IDs:
"Eomes: 13813
FoxA2: 15376
Klf2: 16598
Tcf7l1: 21415"
Figure legend: "(F) ChIP for Tcf7l1 followed by qPCR. Analysis was performed in wild-type ES cells on day 3 of differentiation in the presence or absence of 3 µM CH. Average and SD of three independent experiments. FoxA2 *p = 0.049."
NCBI gene IDs:
"FoxA2: 15376
Tcf7l1: 21415"
Figure legend: "(A) Assay of FoxA2 mRNA by RT-PCR in Tcf7l1 null cells on day 1 to 4 of differentiation without CH. Average and SD of three independent experiments."
NCBI gene IDs:
"FoxA2: 15376
Tcf7l1: 21415"
Figure legend: "(B) Gene expression analysis of endodermal associated genes by RT-PCR in control wild-type and Tcf7l1 null cells on day 4 of differentiation in the presence and absence of 3 µM CH. Average and SD of three independent experiments."
NCBI gene IDs:
"Tcf7l1: 21415"
Figure legend: "(C) FoxA2 (green) and Oct4 (red) immunostaining of Tcf7l1 null ES cells on day 4 of differentiation in the presence or absence of 3 µM CH. DAPI staining in blue."
NCBI gene IDs:
"Tcf7l1: 21415"
Figure legend: "(D) Assay of CXCR4, Hex and Sox17 mRNA by RT-PCR in control wild-type and Tcf7l1 null cells on day 6 of differentiation in the presence and absence of 3 µM CH. Average and SD of three independent experiments."
NCBI gene IDs:
"CXCR4: 12767
Hex: 15242
Sox17: 20671
Tcf7l1: 21415"
Figure legend: "(A) Assay of FoxA2 mRNA by RT-PCR in FoxA2-Tet ES cell lines 48 hr after treatment with the indicated dose of DOX. Average and SD of two independent experiments from 2 independent lines."
NCBI gene IDs:
"FoxA2: 15376"
Figure legend: "B) FoxA2 immunostaining of FoxA2-Tet ES cells 48 hr after DOX treatment. Left, no DOX control. Middle and right, 10ng/ml DOX. DAPI staining shown in blue. Right panel shows middle image without DAPI signal."
NCBI gene IDs:
"FoxA2: 15376"
Figure legend: "(C) Assay of FoxA2 and CXCR4 mRNA by RT-PCR on day 3 of differentiation with DOX at the indicated dose. Average and SD of two experiments from two independent cell lines."
NCBI gene IDs:
"CXCR4: 12767
FoxA2: 15376"
Figure legend: "(D) Assay of Sox17 mRNA by RT-PCR on day 7 of differentiation with addition of 3 µM CH and/or 10 ng/ml DOX at the indicated time points (in days). Average and SD of two independent experiments from two independent cell lines."
NCBI gene IDs:
"Sox17: 20671"
Figure legend: "(B) Assay of Tcf7l1 and FoxA2 mRNA by RT-PCR at day 2 of differentiation in the presence or absence of 3 µM CH or recombinant Wnt3a (200 ng/ml plus 500 ng/ml RSpondin1(WR)). Average and SD of three independent experiments. ** p&lt;0.01."
NCBI gene IDs:
"FoxA2: 15376
Tcf7l1: 21415"
Figure legend: "(C) Assay of Tcf7l1 and FoxA2 mRNA by RT-PCR in Ctnnb1 null cells at day 1 and 3 of differentiation in the absence or presence of 3 µM CH. Average and SD of three independent experiments."
NCBI gene IDs:
"Ctnnb1: 12387
FoxA2: 15376
Tcf7l1: 21415"
Figure legend: "(D) Immunoblot for Tcf7l1 in Ctnnb1 null cells during the first 3 days of differentiation in the absence (-) or presence (+) of 3 µM CH. Results from two independent, clonally-derived Ctnnb1 null ES cell lines are shown. Tubulin was used as a loading control."
NCBI gene IDs:
"Ctnnb1: 12387"
Figure legend: "(A) Assay of cMyc mRNA by RT-PCR in wild-type ES cells at day 1 to 3 of differentiation. Average and SD of three independent experiments."
NCBI gene IDs:
"cMyc: 17869"
Figure legend: "C,D) Immunoblot for the destabilized form of cMyc protein (pThr58) during the first 3 days of differentiation in the absence (-) or presence (+) of 3 µM CH in wild-type and Ctnnb1 null cells respectively."
NCBI gene IDs:
"Ctnnb1: 12387"
Figure legend: "(E) Assay of Tcf7l1 mRNA by RT-PCR in wild-type cells at day 2 of differentiation in the presence or absence of a small molecule inhibitor of cMyc (cMyc Inhibitor II; Myci). Treatment with Myc inhibitor 10058-F4 (Sigma) generated similar results (data not shown). Average and SD of three independent experiments. **p=0.0002."
NCBI gene IDs:
"cMyc: 17869
Tcf7l1: 21415"
Figure legend: "(F) Assay of Tcf7l1 and FoxA2 mRNA by RT-PCR in cMyc null/nMyc heterozygous cells (C-/-;N+/-) and floxed parental cells (cMycfl/fl;nMycfl/fl (control)) at day 3 of differentiation in the presence or absence of 3 µM CH. Average and SD of four or six independent experiments. Tcf7l1 *p=0.01, FoxA2 *p=0.034."
NCBI gene IDs:
"FoxA2: 15376
cMyc: 17869
nMyc: 18109
Tcf7l1: 21415"
Figure legend: "(G) FoxA2 immunostaining of control and cMyc null/nMyc heterozygous (cMyc-/-;nMyc+/-) cells at day 3 of differentiation in the presence of 3 µM CH."
NCBI gene IDs:
"cMyc: 17869
nMyc: 18109"
Figure legend: "(H) Assay of cMyc and Tcf7l1 mRNA by RT-PCR in cMycT58A inducible cell lines (icMycT58A) 24 hr after DOX treatment. Average of 2 independent experiments."
NCBI gene IDs:
"cMyc: 17869
Tcf7l1: 21415"
Figure legend: "(I) Assay of Tcf7l1 mRNA by RT-PCR in icMycT58A clone 1 cell line at day 3 of differentiation in the absence of 3 µM CH following cMyc induction with 10 ng/ml DOX. Average and SD of 3 independent experiments. **p=0.007."
NCBI gene IDs:
"cMyc: 17869
Tcf7l1: 21415"
Figure legend: "(J) Immunoblot for cMyc and Tcf7l1 in icMycT58A clonal cell line 1 48 hr after DOX induction at 10ng/ml."
NCBI gene IDs:
"cMyc: 17869"
Figure legend: "(B) ChIP for cMyc performed in wild-type ES cells differentiated for 2 days in the absence or presence of 3 µM CH. qPCR was carried out for the regions indicated in Fig. 7a. Average and SD for five independent experiments, *p=0.028."
NCBI gene IDs:
"cMyc: 17869"
Figure legend: "(C) ChIP for cMyc performed in wild-type ES cells differentiated for 2 days in the presence of 3 µM CH plus or minus Myc inhibitor 10058-F4. Average and SD for three independent experiments, *p=0.026."
NCBI gene IDs:
"cMyc: 17869"
Figure legend: "(D) Assay for Miz1 mRNA by RT-PCR for the first three days of differentiation in the presence or absence of 3 µM CH. Average and SD of three independent experiments."
NCBI gene IDs:
"Miz1: 22642"
Figure legend: "(E) ChIP for Miz1 or IgG control performed in E14 ES cells differentiated for 2 days in presence of 3 µM CH. qPCR was carried out for the regions indicated in Fig. 7a. Average and SD for five independent experiments, **p=0.0015."
NCBI gene IDs:
"IgG: "
Figure legend: "(C) Survival analysis of mice of the indicated genotypes; number as indicated for each genotype. No statistical difference between genotypes by Log-rank (Mantel-Cox) test. R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4)."
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(E) Peripheral blood leukocyte populations (by lineage) between genotypes at day 0 and day 28. ; R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4). All BM, spleen and thymic analysis at day 28 post tamoxifen treatment"
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(G) Differential analysis of leukocyte populations in the BM. R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4). All BM analysis at day 28 post tamoxifen treatment"
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(H) BM erythroid cells. R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4). All BM, analysis at day 28 post tamoxifen treatment"
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(J) Differential analysis of leukocyte populations in the spleen. R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4). spleen analysis at day 28 post tamoxifen treatment;"
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(K) Thymic cellularity. (L) Differential analysis of thymocyte populations in the thymus. ( R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4). thymic analysis at day 28 post tamoxifen treatment"
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(N) qPCR (SYBR green) based analysis of indicated gene expression in BM. Data expressed as mean +/- SEM gene expression related to Ppia expression. R26-CreERki/+ Adar1fl/+ (control; n=3) and R26-CreERki/+ Adar1fl/P195A (P195A; n=4)."
NCBI gene IDs:
"Adar1: 56417
Cre: 2777477
ER: 2099
R26: 14910
Ppia: 268373"
Figure legend: "(H) survival of pups derived from breeding of a Adar1P195A/P195A to a Adar1+/- animal; number as indicated for each genotype."
NCBI gene IDs:
"Adar1: 56417"
Figure legend: "(K) survival of pups derived from breeding an Adar1P195A/- to an Adar1P195A/+ genotype; number indicated for each genotype. Inset photo: 35-day-old Adar1P195A/- male bred from Adar1P195A/P195A x Adar1+/- parents (data in Panel F); sibling 29-day-old Adar1P195A/+ and Adar1P195A/- male bred from an Adar1P195A/- x Adar1P195A/+ (data in Panel I)."
NCBI gene IDs:
"Adar1: 56417"
Figure legend: "(B) survival of Adar1P195A/E861AIfih1-/- and littermates (all Ifih1-/-)."
NCBI gene IDs:
"Adar1: 56417
Ifih1: 71586"
Figure legend: "(D) survival of Adar1P195A/-Ifih1-/- and littermates (all Ifih1-/-)."
NCBI gene IDs:
"Adar1: 56417
Ifih1: 71586"
Figure legend: "(F) qPCR (SYBR green) analysis of Ifit1 (left) and Irf7 (right) expression on day 14 of analysis."
NCBI gene IDs:
"Ifit1: 15957
Irf7: 54123"
Figure legend: "(H) qPCR (SYBR green) of Ifit1 (left) and Irf7 (right) expression in the cell lines collected at 3 or 24 hours post-treatment with 50 or 1000 U/mL of IFNβ. No statistical difference between genotypes in Ifit1 (left) and Irf7 (right) expression."
NCBI gene IDs:
"Ifit1: 15957
Irf7: 54123"
Figure legend: "(D) Posterior confocal sections of UAS-mCD8-GFP; Ddc-Gal4 adult male brain in combination with UAS-LacZ or UAS-Myc.RNAi-1 stained with antibody to nc82 (pan-neuropil marker; magenta). Scale bars represent 50 μm."
NCBI gene IDs:
"Gal4: 
LacZ: 
Ddc: 35190
Myc: 31310"
Figure legend: "(B) Drosophila S2 cells transfected with the pUAST-HA empty vector or the pUAST-HA-Myc plasmid were co-transfected with each of the firefly luciferase reporter vector bearing the fragment shown in (A). After 48 hours, the luciferase activity was detected in each cell lysate (n = 5 times)."
NCBI gene IDs:
"HA: 
firefly luciferase: 116160065
Myc: 31310"
Figure legend: "(C) Drosophila S2 cells transfected with the pUAST-HA-Myc plasmid or the empty vector were used for quantification of ChIP-PCRs (n = 4 times)."
NCBI gene IDs:
"HA: 
Myc: 31310"
Figure legend: "A. Whole mount in situ hybridization (WISH) of evi1at 20 (left) and 32 (middle) hpf. evi1expression is visible in various structures of the brain, neuronal structures, the posterior pronephric duct (ppnd) and the branchial arches (ba), as well as in the VDA (ventral dorsal aorta) region. Additionally, evi1co-localizes with the endothelial marker flk1 (right)."
NCBI gene IDs:
"flk1: 796537
evi1: 497407"
Figure legend: "B, C. WISH of runx1/c-myb ;in HSPCs (B), mpo in neutrophils (left) and l-plastin (right) in monocytes/macrophages (C) at 36 hpf in control (upper) and evi1 MO (lower) injected embryos.D-G. WISH of globinin erythrocytes of 6 dpf old embryos (D), of rag-1in the thymus of 5 dpf embryos (E; red asterisk), of cd41at 52 hpf (F), and gata2bat 32 hpf (G) for both control (upper) and evi1 morpholino (lower) injected embryos.H. Quantitation of results is shown for each gene, displaying the percentages of embryos with normal vs. changed expression in each condition. A Fisher´s exact test was applied to calculate statistical significance. P &lt; 0.001 (***)."
NCBI gene IDs:
"gata2b: 436962
globin: 30601
cd41: 445380
l-plastin: 30583
evi1: 497407
mpo: 337514
c-myb: 30519
rag-1: 30663
runx1: 58126"
Figure legend: "I. WISH of runx1/c-myb in HSPCs of uninjected control and UAS:mEvi1 plasmid DNA injected Tg(-1.5hsp70l:Gal4) embryos with heat-shock induction performed at 14 hpf.J. WISH of runx1/c-myb in HSPCs of uninjected control and UAS:mEvi1 mRNA in Tg(fli.1:Gal4FF;UAS:RFP) embryos, leading to endothelial-specific evi1 overexpression.K. Graph displays quantitation of results from I and J using the same method as above."
NCBI gene IDs:
"fli.1: 30619
Gal4: 855828
hsp70l: 560210
Evi1: 497407
evi1: 497407
c-myb: 30519
runx1: 58126"
Figure legend: "A. WISH of notch1b(upper) and dll4(lower) in both control (left) and evi1 MO injected embryos (right)."
NCBI gene IDs:
"dll4: 563920
evi1: 497407
notch1b: 794892"
Figure legend: "B. WISH of notch1b in uninjected (left) and UAS:mEvi1 plasmid DNA injected (right) Tg(fli.1:Gal4FF;UAS:RFP) embryos resulting in endothelial-specific evi1 induction."
NCBI gene IDs:
"fli.1: 30619
Gal4: 855828
evi1: 497407
Evi1: 497407
notch1b: 794892"
Figure legend: "A. Confocal time-lapse live imaging was performed in Tg(c-myb:GFP;kdrl:mKate-CAAX) embryos from 28 to 42 hpf (Movie EV 1). Shown are three representative time-points in which the transformation from hemogenic endothelial cells to the hematopoietic fate is visible, indicated by the white arrowheads. For each time-point merged images are shown. White arrowheads denote double-positive cells.B. Cell counts of total numbers of kdrl+cmyb1+ cells \"in view\", dividing and respectively emerging HSPCs. n=10 movies from n=3 biological replicates were analyzed. A non-parametric Mann-Whitney test was used to test for statistical significance and error bars are shown as ± s.d."
NCBI gene IDs:
"GFP: 
mKate: 
kdrl: 796537
c-myb: 30519"
Figure legend: "C. Gene expression analysis of endothelial (kdrl, flt1, dab2, cdh5) and blood specific genes (c-myb, CD45, CD41, runx1) in sorted kdrl+c-myb+ cells from 34 hpf Tg(c-myb:GFP;kdrl:mKate-CAAX) embryos. Cells were isolated from 15-25 pooled embryos per sample. Three biological experiments were performed with one representative shown. Error bars indicate s.d.\u2028of three technical replicates for each representative experiment."
NCBI gene IDs:
"GFP: 
mKate: 
cdh5: 445471
dab2: 404040
flt1: 544667
CD41: 445380
kdrl: 796537
c-myb: 30519
CD45: 559154
runx1: 58126"