EMBO/SourceData huggingface dataset:
Figure legend: "Quantification of SiR-tubulin fluorescence from 69-127 salivary gland derived sporozoites. Note the decrease in the fluorescence intensity of sporozoites expressing α1-tubulin without introns as well as the decreased intensity and length of the chimeras. Linear correlation of sporozoite length and microtubule length reveals a R2=0.94. *, **, and **** indicate p&lt;0.05, p&lt;0.01, and p&lt;0.0001, respectively; ns: not significant; Kruskal-Wallis-test."
NCBI gene IDs:
"α1-tubulin: 3424816"
Figure legend: "Sporozoites with fewest microtubules (α2+++) show a motility defect. n indicates numbers of investigated sporozoites; Numbers on grey bars indicate percentage of partially and persistently moving sporozoites."
NCBI gene IDs:
"α2: 3421492"
Figure legend: "Salivary gland sporozoites contain at least 10 microtubules as revealed by TEM of α2++ infected salivary glands. Green arrowheads point to microtubules. Scale bar: 0.2 µm. Error bars show the full range and boxes 50% of values. Red crosses indicate the mean, red lines the median. Fisher's exact test (two-tailed)."
NCBI gene IDs:
"α2: 3421492"
Figure legend: "MDM were transduced with non-specific scrambled shRNA (shNS) or Beclin-1 shRNA (shBCLN1) and selected using puromycin resistance. Five days later, cells were incubated with 100 pmol/L 1,25D3 or vehicle control for 4 h before infection with HIV and/or M. tuberculosis (TB) for 3 h. Cells were then washed and incubated with 100 pmol/L 1,25D3 or vehicle control for 7 days. (A) Immunoblot analysis performed using antibodies raised to Beclin-1 or β-actin after initial pathogen exposure (Day 0) or after 7 days."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "Inhibition of HIV and M. tuberculosis by 1,25D3 is CAMP and autophagy dependent.MDM were transduced with non-specific scrambled shRNA (shNS) or CAMP shRNA (shCAMP) and selected using puromycin resistance. Five days later, cells were incubated with 100 pmol/L 1,25D3 or vehicle control for 4 h before infection with HIV and/or M. tuberculosis (TB) for 3 h. Cells were then washed and incubated with 100 pmol/L 1,25D3 or vehicle control for 7 days. (A) Immunoblot analysis performed using antibodies raised to CAMP or β-actin after initial pathogen exposure (Day 0) or after 7 days."
NCBI gene IDs:
"CAMP: 820"
Figure legend: "(C-D) Snapshots from confocal live imaging movies of a representative embryo (N=7) during cellularisation co-expressing opto-Notch, Mito-Zdk, MCP::GFP and sim-MS2. Continuous photo-activation from the onset of cellularisation with a stack size of z= 25μm (λ=488 nm) was alternated with mCherry excitation (λ=561 nm) at 1 min intervals. Single confocal z-slices of the mCherry channel are shown to record opto-Notch localization before, and 10 minutes after sustained photo-activation. Magnified insets show opto-Notch bound to the mitochondrial anchor in the dark (C) and in the nucleus upon photo-activation (D). Scale bar, 10 μm."
NCBI gene IDs:
"GFP: 
MCP: 
MS2: 
Zdk: 
Notch: 31293
sim: 41612"
Figure legend: "(D-E) Results of continuous activation experiments in a Hairless heterozygous hypomorphic mutant (Hairless H2) and under overexpression of Twist (Twist OE) The observed outcomes are most consistent with the predictions from the state-dependent inactivation model."
NCBI gene IDs:
"Hairless: 42445
Twist: 37655"
Figure legend: "In situ hybridization of HAND2-AS1 in HCC tumor tissues. HAND2-AS1 staining is shown in tumor tissues (left panel). Quantitation of HAND2-AS1+ cells in liver tissues was examined in 10 high-power fields (HPF) of sections from ten different HCC patients (right panel). Results are shown as means ± SEM. Scale bar, 100 μm."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Macroscopic appearance of livers in 12-month-old DEN-treated lncHand2+/+ and lncHand2 knockout (lncHand2-/-) mice (left panel). Black arrows indicate liver tumors. Quantitation of tumor foci numbers and areas in DEN-treated livers (right panel). Results are shown as means ± SD (n=12)."
NCBI gene IDs:
"Hand2: 15111"
Figure legend: "Representative H&amp;E and immunohistological staining with Ki67 antibody of liver sections of 12-month-old DEN-treated lncHand2+/+ and lncHand2-/- mice. Scale bar, 100 μm."
NCBI gene IDs:
"Hand2: 15111"
Figure legend: "Representative immunofluorescence staining of livers in 5.5-month-old DEN-treated and untreated lncHand2RFP reporter mice for indicated molecules (lower panel). Scale bar, 100 μm. Upper panel: scheme of targeting strategy for IRES-RFP knockin allele."
NCBI gene IDs:
"RFP: 
Hand2: 15111"
Figure legend: "HAND2-AS1 knockout causes a declined oncosphere-forming capacity in HCC cells. The right panel represents statistical results as means ± SD (n=3 per group). Overexpression of HAND2-AS1 (oelnc) rescued the sphere formation reduced by HAND2-AS1 deletion. Scale bar, 100 μm."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "CD13+CD133+ (CSC) subpopulations were detected in HAND2-AS1 knockout cells by FACS analysis. Results are shown as means ± SD (n=4 per group)."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Limiting diluted HAND2-AS1 depletion or Ctrl HCC cells were subcutaneously implanted into BALB/c nude mice. n=12 for each group."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Estimated frequency of TICs in HAND2-AS1 deficiency and control cells after the first transplantation using the extreme limiting dilution analysis. Data are shown as the mean and 95% confidence interval. (n=12 grafted tumors per dilution)."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Estimated frequency of TICs in HAND2-AS1 deficiency and control HCC cells during serial transplantations. Grey area indicates the 95% CI."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Representative whole-body imaging of Huh7-Luc cells transduced with control or HAND2-AS1 KO #1 or KO #2 vectors. Quantification of tumor numbers of 6 weeks after tumor cells orthotopically implanted to B-NSG mice. Data are shown as means ± SD. One-way ANOVA with Dunnett's correction for multiple comparisons."
NCBI gene IDs:
"Luc: 
HAND2-AS1: 79804"
Figure legend: "HAND2-AS1 expression levels (normalized to 18S rRNA) in livers from BALB/c nude mice subcutaneously transplanted xenograft primary HCC cells then intraperitoneally administered 25 mg kg−1 ASOs on days 15, 20, 25, 30 and 35 (n = 5 per group). Three ASOs (1, 2, 3) against HAND2-AS1 were used to treat mice and showed similar depletion effects. Results are shown as means ± SD. n=6 mice per group."
NCBI gene IDs:
"18S rRNA: 
HAND2-AS1: 79804"
Figure legend: "HAND2-AS1 overexpression enhances the capacity of oncosphere formation. Scale bar, 100 μm. oe: overexpression. Right panel: Data are shown as means ± SD (n = 3 per group)."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Estimated frequency of TICs in HAND2-AS1 overexpression and control HCC cells during serial transplantations."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Representative whole-body imaging of Huh7-Luc cells transduced with control or HAND2-AS1 overexpression vectors. Quantification for tumor numbers one month after tumor cells orthotopically implanted to B-NSG mice. (n = 5 mice per group)."
NCBI gene IDs:
"Luc: 
HAND2-AS1: 79804"
Figure legend: "Biotin-RNA pulldowns were performed with nuclear extracts of oncosphere cells using full-length HAND2-AS1 transcript (sense), antisense, and one HAND2-AS1 intron control, followed by mass spectrometry. Band 1: INO80, band 2: RUVBL2."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "HAND2-AS1 was visualized by RNA FISH, followed by immunofluorescence staining of INO80 in HCC primary oncosphere cells. Scale bar, 100 μm."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Mapping analysis of INO80-binding domains of HAND2-AS1. Schematic diagram of HAND2-AS1 full-length and truncated fragments (top panel); Western blot of INO80 and RUVBL2 in RNA pulldown samples by different HAND2-AS1 fragments (middle panel); Different HAND2-AS1 fragments (bottom panel)."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "EMSA of biotin-labeled HAND2-AS1 (nt 83-242) probes incubated with INO80 protein."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Binding affinity of HAND2-AS1 with INO80 was determined by five independent EMSA assays. Non-linear regression curves were generated by GraphPad Prism. Results are shown as means ± SD."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Interaction regions of INO80 with HAND2-AS1 mutations at nt 83-242 of exon 2 were detected using RNA pulldown assays. LR, loop region."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "Representative whole-body imaging of Huh7-Luc cells transduced with control or INO80 shRNA #1 or shRNA #2 vectors. Quantification of tumor numbers of 4 weeks after tumor cells orthotopically implanted to B-NSG mice. Data are shown as means ± SD (n = 5 mice per group). One-way ANOVA with Dunnett's correction for multiple comparisons."
NCBI gene IDs:
"Luc: 
INO80: 54617"
Figure legend: "Macroscopic appearance of livers in 10.5-month-old DEN-treated Ino80flox/flox (Ino80+/+) and Ino80 knockout (Ino80-/-) (left panel). Black arrows indicate tumors. Quantitation of tumor foci numbers and areas in DEN-treated livers. Results are shown as means ± SD (n=10)."
NCBI gene IDs:
"Ino80: 68142"
Figure legend: "Heatmap results for HAND2-AS1 or INO80 deficiency in HCC cells."
NCBI gene IDs:
"HAND2-AS1: 79804
INO80: 54617"
Figure legend: "Based on GSEA results, significantly changed genes due to depletion of HAND2-AS1 and INO80 were attributed to the BMP signaling in HCC primary cells. NES, normalized enrichment score; FWER, familywise error rate."
NCBI gene IDs:
"HAND2-AS1: 79804
INO80: 54617"
Figure legend: "ChIRP-seq analysis of HAND2-AS1 genomic binding at target sites in liver CSCs, using LacZ probes as negative control. A 10-kb interval centered on the called HAND2-AS1 peak is shown."
NCBI gene IDs:
"LacZ: 
HAND2-AS1: 79804"
Figure legend: "Representative ChIRP-seq of HAND2-AS1 binding of BMPR1A."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "Gel analysis of HAND2-AS1 RNA binding BMPR1A promoter in liver CSCs. LacZ and PVT1 served as negative controls."
NCBI gene IDs:
"PVT1: 
LacZ: 
BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "HAND2-AS1 deletion in HCC cells decreases BMPR1A expression and inactivates BMP signaling."
NCBI gene IDs:
"HAND2-AS1: 79804"
Figure legend: "ChIRP-immunoblotting analysis of BMPR1A interaction with the INO80 complex in control and HAND2-AS1-knockout liver CSCs."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "EMSA analysis of the interaction of HAND2-AS1, INO80 and RUVBL2 with the BMPR1A promoter region."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "BMPR1A promoter binding region to HAND2-AS1/INO80 was deleted (BMPR1A-p-KO) in liver CSCs using lenti-Cas9, followed by HAND2-AS1 and INO80 overexpression (oeHAND2-AS1, oeINO80) for 36 h. BMPR1A mRNA levels were examined by realtime PCR. Data are shown as means ± SD. n=5. ** P &lt; 0.01 by two-tailed Student's t test."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804
INO80: 54617
Cas9: 901176"
Figure legend: "BMP signaling was examined in INO80-silenced liver CSCs by Western blot."
NCBI gene IDs:
"INO80: 54617"
Figure legend: "BMPR1A is highly expressed in HCC tumor tissues provided by Wang's cohort (GSE14520)."
NCBI gene IDs:
"BMPR1A: 657"
Figure legend: "BMPR1A overexpression rescues sphere formation ability reduced by HAND2-AS1 depletion in HCC samples. Representative sphere formation is shown on the left panel. Scale bar, 100 μm. Percentages of sphere-forming cells were calculated as means ± SD (right panel). n=4."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "BMPR1A depletion reduces tumor-initiating capacity. n=12 mice per group."
NCBI gene IDs:
"BMPR1A: 657"
Figure legend: "Representative whole-body imaging of Huh7-Luc cells transduced with scramble (Ctrl) or BMPR1A shRNAs. Quantification for tumor numbers of 7 weeks after tumor cells orthotopically implanted to B-NSG mice. Calculated data are shown as means ± SD (n = 5 mice per group)."
NCBI gene IDs:
"Luc: 
BMPR1A: 657"
Figure legend: "Representative H&amp;E and immunohistological staining with Ki67 antibody in liver sections of 10-month-old DEN-treated WT and Bmpr1a-/- mice. Scale bar, 100 μm. Right: Quantitation of Ki67-positive cells in DEN-treated mice. Data are shown as means ± SD. n=5."
NCBI gene IDs:
"Bmpr1a: 12166"
Figure legend: "Representative whole-body imaging of Huh7-Luc cells in different groups. Mice treated with scramble ASOs and/or siRNAs obtained similar results to vehicle treatment. n≥5 mice in each group."
NCBI gene IDs:
"Luc: "
Figure legend: "Quantification of tumor numbers treated with ASOs and/or siRNAs after Huh7-Luc tumor cells orthotopically implanted to B-NSG mice. Calculated data are shown as means ± SD (n = 5)."
NCBI gene IDs:
"Luc: "
Figure legend: "Representative H&amp;E staining in liver sections from tumor-bearing mice treated with ASO1 and siBMPR1A in orthotopic transplants of tumors from HCC#50 and HCC#51. Scale bar, 100 μm."
NCBI gene IDs:
"BMPR1A: 657"
Figure legend: "Representative immunohistological staining with Ki67 antibody of tumor-bearing mice treated with ASO1 and siBMPR1A. Scale bar, 100 μm. Lower panel: Quantitation of Ki67-positive cells in different groups. Data are shown as means ± SD (n=5 per group)."
NCBI gene IDs:
"BMPR1A: 657"
Figure legend: "Northern blotting of HAND2-AS1 and immunobloting of BMPR1A in tumors from PDX HCC#50 after treatment."
NCBI gene IDs:
"BMPR1A: 657
HAND2-AS1: 79804"
Figure legend: "Kaplan-Meier survival analysis of B-NSG mice orthotopically transplanted with PDXs HCC#50 (I) and HCC#51 (J). Treatment with ASO1 combined siBMPR1A causes synergistic therapeutic effect than each treatment alone (n = 10 per group). P value for Kaplan-Meier curves was determined using a Mantel-Cox log-rank test."
NCBI gene IDs:
"BMPR1A: 657"
Figure legend: "A-C Confocal images of Stage 12 embryos  from control, P{GT1}CG9005BG02278 P-element mutant (CG9005PBG), and CG9005PBG with CG9005 expression restored in macrophages. Macrophage: red. Phalloidin to visualize embryo: green. Germband edge: dotted white line. Data information: Scale bars: 50µm (A-C)"
NCBI gene IDs:
"CG9005: 36243"
Figure legend: "D Quantification of macrophages that have penetrated the germband from genotypes in (A-C) and from  CG9005PBG over two deficiencies (Df) that remove the gene. n=35, 56, 25, 9, 18 embryos respectively, p&lt;0.0001 for control vs CG9005PBG, Df1, or Df2; p=0.98 for control vs mac&gt;CG9005 rescue, p=0.91, 0.90 for CG9005PBG vs Df1 or Df2. Data information: Throughout paper mac&gt; indicates GAL4 driven expression of a UAS construct specifically in macrophages by  srpHemo-GAL4. N for movies represents imaging from different embryos. (D) One-way ANOVA with Tukey."
NCBI gene IDs:
"CG9005: 36243
GAL4: 855828
srp: 41944"
Figure legend: "E Macrophage-specific knockdown of CG9005 by UAS-RNAi lines. n=22, 20, 21, 23, 35, 28 embryos. p&lt;0.0001 for all comparisons. Data information: Throughout paper mac&gt; indicates GAL4 driven expression of a UAS construct specifically in macrophages by srpHemo-GAL4. N for movies represents imaging from different embryos. Unpaired t-tests. Graphs show mean±SEM; ns=p&gt;0.05, *p&lt;0.05, ****p&lt;0.0001."
NCBI gene IDs:
"CG9005: 36243
GAL4: 855828
srp: 41944"
Figure legend: "F Stills from two-photon movies of control and CG9005PBG mutant embryos showing macrophages (nuclei, red) migrating starting at Stage 10 from the head towards the germband and invading the germband tissue. Elapsed time indicated in minutes. Germband edge (white dotted line) detected by yolk autofluorescence. Data information: Scale bars: 30µm (F). Throughout this work, embryos with stomadeal invagination and germband retraction away from the anterior of less than 29% were defined as Stage 10, 29%-31% Stage 11, and 35%-40% Stage 12."
NCBI gene IDs:
"CG9005: 36243"
Figure legend: "G-H Macrophage migration speed (G) in the head or (H) between the  yolk sac  and the germband edge. For (G): control n=8 movies, CG9005PBG mutant n=3; control n=360 tracks, mutant n=450, p=0.65. For (H): control n=7 movies, mutant n=3; control n=46 tracks, mutant n=19, p=0.62 Data information: Unpaired t-tests. Graphs show mean±SEM; ns=p&gt;0.05, *p&lt;0.05, ****p&lt;0.0001."
NCBI gene IDs:
"CG9005: 36243"
Figure legend: "I The time required for the first macrophage nucleus to enter into the extended germband. Control n=7 movies, mutant n=5. Time to entry: control=23 min, CG9005PBG=38 min, p&lt;0.0001. Data information: Unpaired t-tests. Graphs show mean±SEM; ns=p&gt;0.05, *p&lt;0.05, ****p&lt;0.0001."
NCBI gene IDs:
"CG9005: 36243"
Figure legend: "C-D Confocal images or (D) quantification of the macrophages in germband in Stage 12 embryos from the control,  atosPBG, and  atosPBG expressing Atos itself or variants lacking particular domains. Transgene expression directly from macrophage specific promoter (mac-). For control (n=32 embryos) vs atosPBG mutant (n=56) p&lt;0.0001; vs rescue with mac-atos (n=18) p&gt;0.99; vs rescue with mac-atosDUF- (n=17) p=0.0003; vs rescue with mac-atosChrSeg-(n=21) p=0.0003; vs rescue with mac-atosDUF-/ChrSeg-(n=19) p=0,00014; vs rescue with mac-atosTAD1-/ TAD2- (n=25) p=0.0009, atosPBG mutant vs. rescue with mac-atos p=0.0031. Data information: Germband edge: dotted white line. Mac- indicates direct expression from the  srpHemo promoter. (C, macrophage nuclei (red), actin by Phalloidin staining (green). (D One-way ANOVA with Tukey. Mean±SEM, ns=p&gt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Scale bars: 50µm (C"
NCBI gene IDs:
"atos: 36243
Atos: 36243
Hemo: 12798465
srp: 41944"
Figure legend: "E Confocal images of atosPBG rescued by expressing Atossa's murine orthologs, mFAM214A or B (mFAMA-B) in macrophages, F Quantification of macrophages in the germband in Stage 12 embryos from the control, atosPBG, and atosPBG embryos expressing mFAM214A or B specifically in macrophages (mac-). For control (n=24 embryos) vs. atosPBG mutant (n=56) p&lt;0.0001; vs mac-atos rescue (n=18) p=0.7; vs mac-mFAMA rescue (n=22) p=0.6; vs mac-mFAMB rescue (n=25) p=0.086. For atosPBG mutant vs mac-atos rescue p=0.0006; vs mac-mFAMA  rescue p=0.0002; vs mac-mFAMB rescue p=0.0043. Data information: Germband edge: dotted white line. Mac- indicates direct expression from the  srpHemo  promoter. E) macrophage nuclei (red), actin by Phalloidin staining (green). F) One-way ANOVA with Tukey. Mean±SEM, ns=p&gt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Scale bars: 50µm E)."
NCBI gene IDs:
"atos: 36243
Atossa's: 36243
FAMA: 235493
FAM214A: 235493
FAMA-B: 230088
FAMB: 230088
srp: 41944"
Figure legend: "B-D Confocal  images of early Stage 12  embryos from the control and lines expressing RNAis against (B)  porthos, (C) GR/HPR or (D) LKR/SDH, specifically in macrophages  (red). Germband edge: dotted white line. Data information: Scale bar: 50µm"
NCBI gene IDs:
"porthos: 35379
GR/HPR: 35347
LKR/SDH: 34064"
Figure legend: "Quantification of the number of germband macrophages in embryos from control and upon RNAi knockdown of the genes In (E) control (n=36 embryos) vs. porthos RNAi (n=28) p&lt;0.0001. In (F) сontrol 1 (n=18 embryos) vs. GR/HPR  RNAi 1 (n=18) and control 2 (n=21) vs. GR/HPR RNAi 2 (n=24), both p&lt;0.0001; control 3 (n=15 embryos) vs. GR/HPR  RNAi 3 (n=23) p=0.08. Data information: Mean±SEM and ns=p&gt;0.05, ****p&lt;0.0001. One-way ANOVA with Tukey"
NCBI gene IDs:
"porthos: 35379
GR/HPR: 35347"
Figure legend: "Quantification of the number of germband macrophages in embryos from control and upon RNAi knockdown of the genes In (G) сontrol 1 (n=18 embryos) vs. LKR/SDH RNAi 1 (n=17) and control 2 (n=21) vs. LKR/SDH  RNAi 2 (n=23), both p&lt;0.0001. Data information: Mean±SEM and ns=p&gt;0.05, ****p&lt;0.0001. One-way ANOVA with Tukey"
NCBI gene IDs:
"LKR/SDH: 34064"
Figure legend: "B Stills starting at Stage 11 from two-photon movies of control embryos and those expressing porthos (pths)-RNAi in  macrophages  (nuclei, red), migrating  from the  head mesoderm towards and into the germband at the indicated  time points. White dotted line: germband edge. Data information: Movies in each analysis set are from independent embryos. Scale bars: 30µm"
NCBI gene IDs:
"porthos: 35379
pths: 35379"
Figure legend: "C-H Quantification of macrophage migration parameters from movies as in (B). Macrophage migration speed (C) in the head or (D) between the yolk sac and the germband  edge. (C) control n=4 movies, pths RNAi n=6; control n=507 tracks,  pths RNAi n=859; p=0.56. (D) control n=5 movies, pths RNAi n=5; control n=40 tracks, pths RNAi n=51; p=0.45. (E) The time required for the first macrophage nucleus to enter into the extended germband: control=23 min, CG9005PBG=38 min, p&lt;0.0001. The migration speed of the (F) 1st, (G) 2nd, or (H) 3rd-5th macrophages  along the first 25-30µm into the germband between the mesoderm and ectoderm: control=21.5 min, n=6, pths RNAi=36.2 min, n=4, p&lt;0.0001. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Unpaired t-test"
NCBI gene IDs:
"CG9005: 36243
pths: 35379"
Figure legend: "I Confocal images of early Stage 12 embryos  from control, atosPBG, and atosPBG expressing atos::FLAG::HA (mac&gt;atos) or  pths::FLAG::HA (mac&gt;pths) in macrophages (red). Embryo detected by phalloidin staining (green). White dotted line: germband  edge.. J Quantification of macrophages  in the  germband  of an atosPBG mutant rescued by expressing pths::FLAG::HA in macrophages. For control (n=15 embryos) vs atosPBG mutant (n=22) and  mac&gt;atos  rescue (n=27) both p&lt;0.0001, vs mac&gt;pths rescue (n=30) p=0.0007. Data information: Movies in each analysis set are from independent embryos. Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. one-way ANOVA with Tukey Scale bars: 50µm"
NCBI gene IDs:
"FLAG: 
HA: 
atos: 36243
pths: 35379"
Figure legend: "E-F Confocal images of early Stage 12 embryos from the controls, from lines expressing  nlacZ  and RNAis against  atos or  pths in macrophages (mac&gt;) as a second control, and lines expressing RNAis targeting atos  or pths along with individual components of the dTORC1  pathway in macrophages, including Nrpl2, Iml1, and TSC1. Germband edge: dotted white line. Data information: Scale bar: 30µm"
NCBI gene IDs:
"lacZ: 
atos: 36243
pths: 35379
Iml1: 38176
Nrpl2: 32677
TSC1: 42862"
Figure legend: "G-H Quantification of the number of germband macrophages in embryos from the controls and upon RNAi knockdown of the genes in E-F. Expression of different RNAis against Nrpl2, Iml1, and TSC1 in atos or pths-depleted macrophages rescues macrophage invasion into the germband while expression of lacZ does not. In (G) control (n=21 embryos) vs. atos RNAi (n=30) p&lt;0.0001; control vs. atos RNAi rescued with Nrpl2 RNAi (n=32) p=0.019, with Iml1 RNAi (n=24) p=0.03, and with TSC1 RNAi (n=15) p=0.04. In (H) control (n=29 embryos) vs pths RNAi (n=26) p&lt;0.0001; control vs. pths RNAi rescued with Nrpl2 RNAi (n=27) p=0.01, with Iml1 RNAi (n=17) p=0.02, and with TSC1 RNAi (n=18) p=0.02. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, ****p&lt;0.0001. one-way ANOVA with Tukey"
NCBI gene IDs:
"lacZ: 
atos: 36243
pths: 35379
Iml1: 38176
Nrpl2: 32677
TSC1: 42862"
Figure legend: "I  Western blot  of protein extracts from control and pths  KD S2R+ probed with p-4EBP1 antibody.  Tubulin serves as a loading control and quantification normalized to loading control. N=3 biological replicates for both control and  pths KD, p=0.69. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, ****p&lt;0.0001. unpaired t-test"
NCBI gene IDs:
"pths: 35379"