EMBO/SourceData huggingface dataset:
Figure legend: "(c, d) Drp1 rescues PINK1KO induced mitochondrial defects in dATG7KO Drosophila. Representative TEM images of indirect flight muscle from PINK1WT (upper panels) and PINK1KO (lower panels) flies combined with dATG7KO or their controls (dATG7WT), followed by expressing either mhc-gal4 (Mhc/+) alone, or Mhc-gal4 driven expression of human Drp1(Mhc&gt;hDrp1WT), mhc-gal4 driven expression of human Drp1S616A(Mhc&gt;hDrp1S616A) or mhc-gal4 driven expression of human Drp1S616D(Mhc&gt;hDrp1S616D) are shown (c). Scale bar=1 μm. Normal and abnormal mitochondrial number per mm2 from each experimental group were and quantified. &gt;3 flies/group and 3-6 pictures of different microscopic fields from each fly were analyzed per repeat. Bars represent the mean ± SEM of three independent experiments (d)."
NCBI gene IDs:
"ATG7: 37141
Drp1: 10059
Drp1: 74006
gal4: 855828
PINK1: 31607"
Figure legend: "(e). Drp1 rescues PINK1KO induced ATP reduction in Drosophila. ATP contents of thorax muscle tissues from the indicated genotypes were measured and normalized against the protein levels One-way ANOVA followed with Tukey's test. ***p&lt;0.001, ns: no significance. Data was presented as mean ± SEM of three independent experiments."
NCBI gene IDs:
"Drp1: 10059
PINK1: 31607"
Figure legend: "(g, h). Reduction of Drp1S616 phosphorylation in dermal fibroblasts derived from PD patients with PINK1 mutations. Lysates of cells derived from three unrelated normal control individuals (Control, C) and four familial PD patients with PINK1 mutations (PINK1mutant) were immunoblotted with antibodies recognizing either phosphor-Drp1S616 (pS616) or Drp1 protein. β-actin was detected as a loading control (g). A quantitative analysis of phospho-Drp1S616 (pS616/Drp1) is shown (h). Student's test. **p&lt;0.01, ns: no significance. Data was presented as mean ± SEM of three independent experiments."
NCBI gene IDs:
"PINK1: 65018"
Figure legend: "Human bronchial epithelial BEAS-2B cells were infected with the influenza A/Scotland/20/74 (H3N2) virus (IAV) at MOI=1 for 4 h, and subsequently treated or not with 4 mg/mL of succinate  (Suc) for 20 h. (e) Human bronchial epithelial BEAS-2B  cells were infected with a A/Scotland/20/74 (H3N2) virus  (IAV) carrying a wild-type NP  (NP  WT) or with the corresponding mutated virus bearing a NP  with a K87R substitution (NP  K87R). Cells were infected by either virus at MOI=1 for 4 h, then washed and treated or not with 4 mg/mL of succinate  for 20 h. Localization  of NP  proteins was analyzed by confocal immunofluorescence microscopy. Scale bar: 10 µM. Pictures are representative of three independent experiments. (f) Relative nuclear  intensity of NP  was determined by using the Intensity Ratio Nuclei Cytoplasm  Tool. Data information: Data are represented as the mean ± SEM of 3 biological replicates without 2 technical replicates each. at least 10 (f) random images were analysed per treatment with a minimum of 5 cells per field. Statistical analysis was performed using the Mann-Whitney test (*P &lt; 0.05)."
NCBI gene IDs:
"NP: 3655155"
Figure legend: "A. Levels of ROW protein in rowRNAi lines were decreased in both male and female fly heads. Western blot for the two rowRNAi lines compared to controls was performed using rat polyclonal αROW and rat αTUBULIN (TUB). Genotype description: rowRNAi-1(Act-GAL4/+; UAS-rowRNAi-1/+), rowRNAi-2(Act-GAL4/+; UAS-rowRNAi-2/+), rowRNAi-1 Control (CyO/+; UAS-rowRNAi-1/+), rowRNAi-2 Control (CyO/+; UAS- rowRNAi-2) and Act-GAL4 Control (Act-GAL4 /+)."
NCBI gene IDs:
"Act: 5656844
GAL4: 855828
row: 36685
CyO: 34350"
Figure legend: "B. Decrease in the viability of rowRNAi lines. To examine the viability of rowRNAi flies, we counted the offspring generated from the cross between the heterozygous Act-GAL4/CyO driver line with the homozygotes rowRNAi, or WT (w1118) flies as control. Values are the percentages from the total progeny ± standard error of the mean (SEM)."
NCBI gene IDs:
"Act: 5656844
GAL4: 855828
row: 36685
CyO: 34350"
Figure legend: "C. Decrease in pupal eclosion of rowRNAi line. Pupae were collected from the cross between the heterozygous Act-GAL4/CyO driver line with the homozygotes rowRNAi-1 or WT (w1118) flies as control. Values are the percentages for each genotype of pupal eclosion ± SEM."
NCBI gene IDs:
"Act: 5656844
GAL4: 855828
row: 36685
CyO: 34350"
Figure legend: "D. Survival curve for rowRNAi flies showing reduced life span compared to controls. n=3 (biological replicates). Significance was tested using the Kaplan-Meier estimate of survival and log-rank test."
NCBI gene IDs:
"row: 36685"
Figure legend: "E. Crossing of females/males rowRNAi flies with WT males/females (w1118) flies resulted in a strong reduction in offspring number. Values are the number of offspring from crosses between WT (w1118) flies and flies with different genotypes."
NCBI gene IDs:
"row: 36685"
Figure legend: "A. Western blot for flies expressing endogenous FLAG-tagged ROW (ROW-FLAG), with αFlag tag antibody to validate the tagging. αTubulin (TUB) antibody was used as a reference. Het, Heterozygotes; Hom, Homozygotes for tagged ROW."
NCBI gene IDs:
"ROW: 36685"
Figure legend: "D. BEAF-32 coimmunoprecipitate with ROW. Lysates from S2 cells not transfected (-) or transfected (+) with ROW-FLAG tagged plasmid were subjected to immunoprecipitation with αFlag tag beads. The immunoprecipitates and input (5%) were analyzed by western blot with αFlag tag and αBEAF-32 antibodies."
NCBI gene IDs:
"FLAG: 
ROW: 36685"
Figure legend: "D. ChIP-qPCR results using FLAG-tag antibody for S2 cells transfected with WT or AT-hook mutant ROW-FLAG tagged plasmids (n = 3, technical and biological replicates). As a control, cells not transfected with ROW-FLAG tagged plasmid were used. Input percentages for 3 biological replicates are shown for three promoters containing AT-rich sequences (Hcf, Phax, and Hcs)."
NCBI gene IDs:
"FLAG: 
Hcf: 43788
Hcs: 40659
Phax: 35926
ROW: 36685"
Figure legend: "E. Co-IP results using αFlag antibody in S2 cells transfected with WT or AT-hook mutant ROW-FLAG tagged plasmids or with a plasmid containing only the ZNF domains of ROW (with FLAG-tag). As a control, cells not transfected with ROW-FLAG tagged plasmid were used. Western blot using αFLAG, αWOC, αBEAF-32, αHP1c, and αTUB (loading control) antibodies is shown for input and IP samples."
NCBI gene IDs:
"FLAG: 
ROW: 36685"
Figure legend: "(B) Quantitative analysis of AEP positive cells and C/EBPβ positive cells, respectively. The density of both AEP positive cells and C/EBPβ positive cells were significantly decreased through knocking down C/EBPβ or knocking out AEP in mice. Representative data of five samples, data are shown as mean± SEM. *P =0.0390 (*P &lt; 0.05), ***P =0.0002 (***P &lt; 0.001), ****P &lt; 0.0001, one-way ANOVA. (C) Quantitative analysis of AT8 positive cells (left) and TauN368 positive cells (right), respectively. The density of both AT8 positive cells and TauN368 positive cells were significantly decreased through knocking down C/EBPβ or knocking out AEP in mice. n=5 in each group, data are shown as mean± SEM. *P =0.0194 (*P&lt; 0.05), ***P =0.0001 (***P &lt; 0.001), ****P &lt; 0.0001, one-way ANOVA. (D) Quantitative analysis of Aβ positive cells (left) and APPC586 positive cells (right), respectively. The fluorescence intensity of Aβ positive cells and the density APPC586 positive cells were significantly decreased through knocking down C/EBPβ or knocking out AEP in mice. Representative data of five samples, data are shown as mean± SEM. *P=0.0107 (*P &lt; 0.05), **P=0.0048 (3xTg vs 3xTg AEP KO), **P=0.0044 (3xTg vs WT) (**P&lt; 0.01), ***P=0.0006 (***P&lt; 0.001), ****P &lt; 0.0001, one-way ANOVA. (E) Quantitative analysis of AT8 positive cells (upper) and T22 positive cells (lower), respectively. The fluorescence intensity of both AT8 positive cells and T22 positive cells were significantly decreased through knocking down C/EBPβ or knocking out AEP in mice. Representative data of five samples, data are shown as mean± SEM. **P=0.0014 (3xTg vs 3xTg C/EBPβ+/-), **P=0.0030 (3xTg vs 3xTg AEP KO) (**P&lt;0.01), ***P=0.001, ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"C/EBPβ: 12608
AEP: 19141"
Figure legend: "(C) Detection of phosphorylation PIN2 by Phos-tag Western blotting. Arabidopsis plants (Col-0) were growth in ammonium as only nitrogen source, and treated with 5 mM KNO3 or 5mM KCl as control. Total protein from roots were analyzed in SDS PAGE using Phos-tag to detect changes in phosphorylation status. Immunoblotting was performed with PIN2 antibody. Total proteins isolated from eir1.1 roots were used as a negative control. White and red asterisks indicate a slow- or fast-mobility band corresponding to a more or less phosphorylated PIN2, respectively."
NCBI gene IDs:
"eir1.1: 835813"
Figure legend: "(D) Western blot against PIN2 protein comparing nitrate treated (KNO3) and control (KCl) condition in Arabidopsis roots for all genotypes (eir1-1 mutant background was complemented with PIN2::PIN2wt-GFP (PIN2wt), PIN2::PIN2S439D-GFP (PIN2S439D phospho-mimic point mutation) or PIN2::PIN2S439A-GFP (PIN2S439A, phospho-null point mutation)."
NCBI gene IDs:
"eir1-1: 835813
PIN2: 835813"
Figure legend: "(A) Primary root length of Col-0 wild type plants or eir1-1 mutant plants was measured using the ImageJ program 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10-15 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Asterisk indicates statistically significant difference between analyzed by unpaired, two-tailed and assuming equal variance t-test (** p &lt; 0.01)."
NCBI gene IDs:
"eir1-1: 835813"
Figure legend: "(B) Number of initiating and emerging lateral roots of Col-0 or eir1-1 mutant plants were counted using light microscopy 3 days after 5 mM KNO3 or KCl treatments. Tukey box plot show results from 3 independent biological replicates per experimental condition (n = 10-15 roots each replicate). The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Asterisk indicates statistically significant difference between analyzed by unpaired, two-tailed and assuming equal variance t-test (** p &lt; 0.01)."
NCBI gene IDs:
"eir1-1: 835813"
Figure legend: "(A) Confocal microscopy using Zeiss Airyscan microscope and Zeiss-blue3.1 software was used to visualize PIN2 in Arabidopsis roots. \"e\" denotes epidermis and \"c\" cortex. Scale bar = 20 µm. (B-D) Tuckey box plots show PIN2-GFP fluorescence intensity quantification (number of roots analyzed per experimental conditions = 8-10; arbitrary units, a.u.) at the total cell membrane in roots (B), and from epidermal (C) and cortex (D) plasma membrane in the different genotypes. The box plot shows the data within the interquartile range (25th and 75th percentiles) and a solid black line represents the median. Whiskers show maximum and minimum values no further than 1.5x IQR (interquartile range). Outlier data are plotted individually. Letters denote statistically significant difference between means as determined by one-way ANOVA analysis followed by Tukey HSD post hoc test (p &lt; 0.05). "
NCBI gene IDs:
"GFP: 
PIN2: 835813"
Figure legend: "(A) Immunoblot  analysis of SP  in lysates from COS-7  cells which were untransfected, transfected with RFP-SP  + empty vector or co-transfected with SP-shRNA-GFP  + RFP-SP  (knock-down) or shRNA-resistant RFP-SP  (rescue). β-actin  and GFP  signals illustrate equal protein loading and SP-shRNA expression level, respectively. (B) Quantification of SP  expression from 6 different experiments, normalized to the  β-actin signal. *P &lt; 0.05, ns, not significant, P &gt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). Data information: Data represent mean ± SEM."
NCBI gene IDs:
"GFP: 
RFP: 
SP: 
SP: 104027"
Figure legend: "(C) Homer-DsRed  (green) and immunostained  endogenous SP  (red) in dendrites  from neurons  transfected with either empty vector, scramble shRNA or SP-shRNA with GFP  reporter (blue). Scale bar: 10 µm. (D) Average SP  intensity for same conditions (EV: n = 47; Scr-shRNA: n = 53; SP-shRNA: n = 62, n indicates the number of cells, from 3 cultures; normalized to empty vector condition). **P &lt; 0.01, ****P &lt; 0.0001 (Kruskal-Wallis test followed by Dunn's multiple comparison test). (E) Fraction of SP+ synapses  for same conditions. (EV: n = 47; Scr-shRNA: n = 53; SP-shRNA: n = 62, n indicates the number of cells from 3 cultures). *P &lt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). Data information: Data represent mean ± SEM."
NCBI gene IDs:
"GFP: 
SP: 60324"
Figure legend: "(F) Homer1c-DsRed (red) and immunostained  surface AMPARs  (green) in dendrites  from neurons  transfected with either SP-shRNA-GFP  or empty vector (EV) with GFP   reporter (blue) and treated with TTX  or left untreated (UT). Scale bar: 5 µm. (G) AMPAR synaptic  fluorescence intensity normalized to untreated empty vector (EV) condition (EV: UT, n = 42, TTX, n = 33; SP-shRNA: UT, n = 30, TTX, n = 31, n indicates the number of cells, from 3 cultures). *P &lt; 0.05, **P &lt; 0.01, ns, not significant, P &gt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). Data information: Data represent mean ± SEM."
NCBI gene IDs:
"GFP: 
SP: 60324"
Figure legend: "(A) Micrographs showing Homer1c-GFP  (green) and immunostaining  for surface AMPARs  (blue) and endogenous SP  (red) in neurons  transfected with miR-124  or control miR-67 (miR-Ctrl), and treated with TTX, or left untreated (UT). Scale bar: 5 µ (B) Percentage of SP+ synapses  for same conditions as in (A) (miR-Ctrl: UT, n = 25, TTX, n = 26; miR-124: UT, n = 30, TTX, n = 30, n indicates the number of cells, from 3 cultures). ****P &lt; 0.0001, ns, not significant, P &gt; 0.05 (two-way ANOVA test followed by Tukey's multiple comparison test). (C) Homer1c-GFP  intensity for same condition as in (A), normalized to untreated miR-Ctrl (miR-Ctrl: UT, n = 25, TTX, n = 26; miR-124: UT, n = 30, TTX, n = 30, n indicates the number of cells, from 3 cultures). ns, not significant, P &gt; 0.05 (two-way ANOVA test followed by Tukey's multiple comparison test). (D) AMPAR synaptic  fluorescence intensity for same condition as in (A), normalized to untreated miR-Ctrl (miR-Ctrl: UT, n = 25, TTX, n = 26, miR-124: UT, n = 30, TTX, n = 30, n indicates the number of cells, from 3 cultures). ***P &lt; 0.001, *P &lt; 0.01, ns, not significant, P &gt; 0.05 (two-way ANOVA test followed by Tukey's multiple comparison test). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"miR-67: 259740
miR-124: 70441///268755"
Figure legend: "(A) Micrographs showing dendrites  from neurons  transfected with Homer1c-DsRed  (magenta) and SEP-GluA2  constructs containing wild-type (WT) or mutated (MUT) 3'UTR (green) and treated with TTX  for 48 h, or left untreated (UT). Scale bar: 10 µm. (B) SEP-GluA2 synaptic  fluorescence intensity for each condition, normalized to GluA2-3'UTR-WT untreated neurons  (3'UTR-WT: UT, n = 29, TTX, n = 37; 3'UTR-MUT: UT, n = 18, TTX, n = 14; n indicates the number of cells, from 3 cultures). *P &lt; 0.05, ns, not significant, P &gt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"SEP: 
GluA2: 29627"
Figure legend: "(C) Micrographs showing dendrites  from neurons  transfected with Homer1c-BFP  (magenta), GFP-SP-shRNA (gray) and a rescue RFP-SP  construct containing wild-type (WT) or mutated (MUT) 3'UTR (geen) and treated with TTX, or left untreated (UT). Scale bar: 10 µm. (D) Percentage of SP+ synapses  for each condition (3'UTR-WT: UT, n = 26, TTX, n = 28; 3'UTR-MUT: UT, n = 26, TTX, n = 24; n indicates the number of cells, from 3 cultures). **P &lt; 0.01, ***P &lt; 0.001, ns, not significant, P &gt; 0.05 (two-way ANOVA test followed by Tukey's multiple comparison test). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"GFP: 
RFP: 
SP: 104027
SP: 60324"
Figure legend: "(E) Micrographs showing Homer1c-GFP  (magenta) and immunostaining  for surface AMPARs  (cyan) and endogenous SP  (green) in neurons  transfected with or without 50 nM SP TSB-LNA and treated with TTX, or left untreated (UT). Scale bar: 10 µm. (F) Percentage of SP+ synapses  for each condition (Control: UT, n = 36, TTX, n = 52; SP TSB-LNA: UT, n = 62, TTX, n = 29; n indicates the number of cells from 3 cultures). *P &lt; 0.05, **P &lt; 0.01, ns, not significant, P &gt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). (G) AMPAR synaptic  fluorescence intensity for each condition, normalized to control untreated neurons  (Control: UT, n = 30, TTX, n = 36; SP TSB-LNA: UT, n = 37, TTX, n = 20; n indicates the number of cells from 2 cultures). *P &lt; 0.05, ns, not significant, P &gt; 0.05 (Kruskal-Wallis test followed by Dunn's multiple comparison test). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"SP: 60324"
Figure legend: "(A) Confocal  images showing dendrites  from CA3 pyramidal cells  transfected with RFP-SP  (green) containing wild-type (WT) or mutated (MUT) 3'UTR + SP-shRNA-BFP  (magenta). Arrowheads indicate SP+ spines. Scale bar: 10 µm. (B) Graph plot showing spine head volume vs SP  integrated intensity (n = 5 cells). Each plot represents a single spine  for which SP  integrated intensity has been normalized to average SP  intensity of SP+ spines  in the same neuron. Plots for SP- and SP+ spines  appear in grey and magenta, respectively. The black dotted line represents linear regression (R2 = 0.25, P = 0.0004). (C) Paired data showing spine head volume for SP- vs SP+ spines. Each pair of plot represents average values for SP- vs SP+ spines  for a given neuron. *P = 0.0031 (two-tailed Student t test). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"BFP: 
SP: 60324"
Figure legend: "(F) Higher magnification of dendritic spines from neurons  transfected with rescue RFP-SP  (magenta) containing wild-type (WT) or mutated (MUT) 3'UTR and BFP-shRNA-SP   (grey) and contacted by GFP-Syp+ terminals  (green). Arrowheads indicate putative synaptic  contacts. Scale bar: 5 μm. (G) Percentage of SP+ spines  in neurons  expressing RFP-SP-3'UTR-WT vs -MUT and depending on the apposition or not to a GFP-Syp+ terminal  (SP-3'UTR-WT: TetTx-, n = 220, TetTx+, n = 26; SP-3'UTR-MUT: TetTx-, n = 616, TetTx+, n = 25; n indicates the number of spines  from 5-7 pairs). (H) Spine head volume in same conditions as in (G) (SP-3'UTR-WT: TetTx-, n = 26, TetTx+, n = 26 spines  from 5 pairs, 4 slices; SP-3'UTR-MUT: TetTx-, n = 25, TetTx+, n = 25; n indicates the number of spines  from 7 pairs, 7 slices). ***P &lt; 0.001, *P &lt; 0.05, ns, not significant (Kruskall-Wallis test followed by Dunn's multiple test). (I) Cumulative probability distributions of spine head  volumes for TetTx- and TetTx+ spines  from neurons expressing RFP-SP-3'UTR-WT (SP-WT, light gray and light green) or RFP-SP-3'UTR-MUT (SP-MUT, dark gray and dark green). Data information: Data are represented as mean ± SEM."
NCBI gene IDs:
"RFP: 
SP: 104027
TetTx: 24255210"
Figure legend: "D. HeLa cells were transfected with Nup358 siRNA (siNup358) or Nup214 siRNA (siNup214). Cells were fixed and stained for endogenous Nup358 (green, upper panel) or Nup214 (green, lower panel) and endogenous P body marker (Dcp1a, red). Graph represents the quantitative data as mean ± SD. The data was obtained from three independent experiments, and in each experiment, 100 cells were counted from different fields for the presence of intact microscopically distinct P bodies and expressed as percentage."
NCBI gene IDs:
"Nup214: 8021
Nup358: 5903"
Figure legend: "D. HeLa cells were transfected with Nup358 siRNA (siNup358) or Nup214 siRNA (siNup214). Western blot (WB) indicates the extent of Nup358 depletion and the level of Dcp1a."
NCBI gene IDs:
"Nup214: 8021
Nup358: 5903"
Figure legend: "A. Western analysis of HeLa cells, treated with control (siControl), Dicer (siDicer), Nup214 (siNup214) or Nup358 (siNup358) siRNA, for assessing the extent of protein depletion using indicated antibodies. Vinculin was used as loading control."
NCBI gene IDs:
"Dicer: 23405
Nup214: 8021
Nup358: 5903"
Figure legend: "B. HeLa cells were initially transfected with indicated siRNAs, followed by renilla luciferase (RL) reporter constructs; RL-control (no let-7a binding site in the 3'UTR) or RL-3xBulge (3 imperfect let-7a binding sites in the 3'-UTR). Firefly luciferase (FL) was co-transfected to serve as internal control. RL/FL luminescence ratio was calculated. Data are presented as mean SD (n = 3), P‐value was calculated using Student's t‐test."
NCBI gene IDs:
"Firefly luciferase: 
renilla luciferase: 
let-7a: 406883///406882///406881"
Figure legend: "C. HEK293T cells were transfected with indicated siRNAs, followed by pCMV-FL-miR30 (P) reporter with either pSUPER-control or pSUPER-miR30 constructs. RL was co-transfected as internal control. FL/RL luminescence ratio was calculated. Data are presented as mean SD (n = 3), P‐value was calculated using Student's t‐test."
NCBI gene IDs:
"miR30: "
Figure legend: "D. HeLa cells were initially transfected with control (siControl), Dicer (siDicer) or Nup358 (siNup358) specific siRNAs, followed by FL constructs containing wild type (HMGA2-wt) or mutant (HMGA2-mut) HMGA2 3'-UTR, along with RL as internal control. Graph was plotted using data from three independent experiments. FL/RL luminescence ratio was calculated. Data are presented as mean SD (n = 3), P‐value was calculated using Student's t‐test."
NCBI gene IDs:
"Dicer: 23405
HMGA2: 8091
Nup358: 5903"
Figure legend: "A. HeLa cells were transfected with control, Dicer or Nup358 specific siRNAs, as indicated. Upper panel, total RNA was isolated and analyzed by northern blotting for let-7a using radio-labeled probe. Ethidium bromide (EtBr) stained gel indicates equal loading of RNA samples."
NCBI gene IDs:
"Dicer: 23405
let-7a: 406883///406882///406881
Nup358: 5903"
Figure legend: "A. HeLa cells were transfected with control, Dicer or Nup358 specific siRNAs, as indicated. Bottom panel, total RNA was isolated from control, Dicer or Nup358 siRNA treated cells and was reverse-transcribed using TaqMan microRNA reverse transcription kit. The levels of let-7a were quantified by qPCR following manufacturer's instructions. Graph represents the relative levels of miRNAs as compared to U6 RNA control. The values were further normalized to let-7a levels in control siRNA knockdown condition. Data are presented as mean SD (n = 3)."
NCBI gene IDs:
"Dicer: 23405
let-7a: 406883///406882///406881
Nup358: 5903"
Figure legend: "B. HeLa cells were transfected with indicated siRNAs, followed by RL-3xBulge construct. Immunoprecipitation (IP) was performed with control mouse IgG (IgG IP) or anti-AGO2 antibody (AGO2 IP) using lysates prepared from these cells. Total RNA was isolated from control and AGO2 immunoprecipitates and the levels of AGO2 associated let-7a (top left panel) and miR-17 (top right panel) were quantified by q-PCR. Graph represents the extent of miRNAs associated with AGO2 as compared to U6 RNA (as a negative control) with AGO2."
NCBI gene IDs:
"miR-17: 406952
let-7a: 406883///406882///406881"
Figure legend: "B. HeLa cells were transfected with indicated siRNAs, followed by RL-3xBulge construct. Immunoprecipitation (IP) was performed with control mouse IgG (IgG IP) or anti-AGO2 antibody (AGO2 IP) using lysates prepared from these cells. Bottom left panel; RNA isolated from IgG control or AGO2 immunoprecipitate samples was reverse-transcribed using oligo(dT) primer. RL-3xBulge mRNA association with AGO2 was quantified by q-PCR and normalized to the level of GAPDH mRNA associated with AGO2."
NCBI gene IDs:
"GAPDH: 
AGO2: 27161"