EMBO/SourceData huggingface dataset:
Figure legend: "G: PCR diagnostic for the presence of modified nth alleles in blood stage infections obtained after sporozoite transmission of a NTH∆PP x LAP3/mCherry genetic cross (lane NTH∆PP), amplifying an approximately 4.4kb fragment. NTH/GFP parasites are used as a PCR control, amplifying approximately 4.6kb. Parental parasites provide a negative control."
NCBI gene IDs:
"GFP: 
LAP3: 
mCherry: 
nth: 3424314
NTH: 3424314"
Figure legend: "A: Brightfield and fluorescence image of an unsporulated NTH/GFP oocyst at 15 days post-infection. Scale bar = 20μm. B: Image of a sporulated NTH/GFP oocyst at 15 days post-infection. Scale bar = 20μm. "
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "C: Image of a NTH/GFP sporozoite, showing NTH::GFP localisation in a tubular structure. Scale bar = 10μm."
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "E: Image of sporozoites expressing NTH::GFP (green) and ACP::mCherry (red), showing co-localization in the apicoplast (arrows). Sporozoites expressing only ACP::mCherry or NTH::GFP are also present. Scale bar = 10μm."
NCBI gene IDs:
"GFP: 
mCherry: 
ACP: 3424006
NTH: 3424314"
Figure legend: "Purified CUL3WT and CUL3Δ403-459 analysed by SDS-PAGE and Coomassie blue staining, following expression in SF21 cells, and DAC-tag-affinity purification and tag cleavage (see Materials and Methods). Bands migrating between 62- and 98-kDa protein markers are CUL3WT and CUL3Δ403-459, respectively, while the band below 17 kDa is Rbx1. The immunoblot for RBX1 is shown on the right. The band in CUL3WT denoted with * is co-purified insect cell CAND1, which was later removed by SEC. All protein identities were confirmed by mass spectrometry."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "KLHL3 binding to CUL3WT and CUL3Δ403-459 is comparable as shown by pull-down assays with a KLHL3 resin generated from cross-linking anti-KLHL3 antibody to Protein G sepharose, and then binding saturating amounts of KLHL3 to the anti-KLHL3 resin (denoted KLHL3R), the control resin (ControlR) is Protein G sepharose (see Materials and Methods for further detail). CUL3WT or CUL3Δ403-459 was incubated with either KLHL3R or ControlR at 4°C for 1 h and then washed thrice with 1× PBS, resin volumes were minimised, and SDS-Laemmli buffer was used to elute protein from resin prior to analysis by SDS-PAGE and staining with colloidal blue."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "UBE2D3˜ubiquitin-release assays analysed by non-reducing SDS-PAGE and immunodetection. His-tagged UBE2D3 was charged with ubiquitin by incubation with ATP and UAE, and charging was stopped by ATP depletion with apyrase. No CUL3 (negative control, lane 1), CUL3WT or CUL3Δ403-459 was then added to the charged E2 and the activity of the RING domain, RBX1, monitored by the hydrolysis of ubiquitin from UBE2D3, visualised by immunodetection with anti-His antibody (upper panel, \"˜\" denotes thioester bond). The lower panel is an immunoblot for CUL3, showing equivalent amount of both CUL3WT and CUL3Δ403-459 was added to the reaction mix; over time, the appearance of higher molecular weight bands represents the modification of CUL3 by covalent attachment of ubiquitin. The right two lanes are control lanes incubated for 10 min with E2˜Ubi but then treated with reducing agent prior to SDS-PAGE."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "Time course of in vitro neddylation reactions. Purified NAE, UBE2M and Nedd8 were mixed with either CUL3WT or CUL3Δ403-459 and incubated at 30°C for the time indicated. Due to the covalent attachment of Nedd8 (8.6 kDa), modified proteins have decreased mobility. The band shift observed for CUL3WT (lane 1 versus lane 2) is representative of a single Nedd8 modification, while the multiple bands in the CUL3Δ403-459 reaction reflect the attachment of up to three Nedd8 molecules (lane 5 versus lane 8)."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "HEK-293 cells over-expressing either FLAG-CUL3WT or FLAG-CUL3Δ403-459 were immunoprecipitated with M2 Flag-binding sepharose, in the presence of a deneddylase inhibitor (OPT). Extract, cells were lysed in the presence of OPT and clarified by centrifugation. IP:M2, clarified supernatant samples were incubated for 1 h at 4°C with M2 (anti-Flag) sepharose, then washed with PBS and eluted with SDS-Laemmli buffer, prior to SDS-PAGE and immunodetection with anti-FLAG antibody. Bands are labelled as follows: 1. CUL3WT, 2. CUL3WT-N8, 3. CUL3Δ403-459, 4. CUL3Δ403-459-N8, 5. CUL3Δ403-459-2(N8)."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "Neddylation in HEK-293 cells over-expressing FLAG-CUL3WT or FLAG-CUL3Δ403-459 was blocked by treatment with a Nedd8-E1-enzyme inhibitor, 3 μM MLN4924 (Millennium Pharmaceuticals), in cells over-expressing FLAG-CUL3WT and FLAG-CUL3Δ403-459 respectively. Cell extracts were sampled over time in the presence of OPT to prevent de-neddylation and subjected to SDS-PAGE and immunodetection of the FLAG tag."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "FLAG-CUL3WT or FLAG-CUL3Δ403-459 was immunoprecipitated with M2-sepharose as described in (F). +MLN4924 indicates cell culture media supplementation with 3 μM MLN4924 for 5 h prior to cell lysis to achieve complete deneddylation of CUL3. Following SDS-PAGE, immunoblotting with COP9 Signalosome (CSN) or CAND1 antibodies determined the interaction between these Cullin regulators and FLAG-CUL3WT and FLAG-CUL3Δ403-459, respectively."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "GST-CAND1 pull-down assays with CUL3 proteins. Purified GST or GST-CAND1 immobilised on glutathione-sepharose was mixed with either purified CUL3WT or CUL3Δ403-459 and incubated at 4°C for 1 h. The sepharose was then washed thrice and eluted from the resin by incubation with SDS-Laemmli buffer prior to SDS-PAGE and Coomassie staining. CUL3WT but not CUL3Δ403-459 interacts with CAND1."
NCBI gene IDs:
"CAND1: 55832
CUL3: 8452"
Figure legend: "A-D In vitro ubiquitylation assays with purified proteins. As ubiquitin is covalently attached to the substrate lysine residue, the appearance of higher molecular weight protein bands reflects the modification of the protein with ubiquitin. All assays contain purified UBE1, UBE2D3, ubiquitin, 0.1 mM ATP, 1 mM MgCl2 and were buffered in 50 mM HEPES, 150 mM NaCl and incubated at 30°C for the time indicated. Reactions were stopped by the addition of SDS-Laemmli buffer to a concentration of 1×. SDS-PAGE, staining with Coomassie blue, or detection with the indicated antibody following immunoblotting enabled the visualisation of ubiquitylation. (A) to determine the relative modification of KLHL3 by CUL3WT and CUL3Δ403-459, KLHL3 was included into the reaction at 2× molar concentration over CUL3WT and CUL3Δ403-459. (B, C) Reactions serve to determine basal auto-ubiquitylation of CUL3WT or CUL3Δ403-459 and do not contain KLHL3 or other potential substrate proteins. Lysine residues on CUL3WT or CUL3Δ403-459 act as the substrate. (B) High molecular weight bands reflect ubiquitin chain linkages on CUL3 or the multiple mono-ubiquitylation of a number of CUL3 lysine residues. (C) Activity assays contain methylated ubiquitin, a form of ubiquitin incapable of forming ubiquitin chains; as such, higher band shifts reflect the attachment of mono-ubiquitin to one more lysine residue on CUL3WT or CUL3Δ403-459, respectively. (D) Activity assay performed as in (C) with methyl-ubiquitin, the boxes shown on the gel are indicative of the gel pieces excised for mass spectrometry analysis in (F)."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "A-C In vitro ubiquitylation assays were performed as described in Figure 2, but with the addition of immunoprecipitated over-expressed FLAG-WNK4 in (B, C) (see Materials and Methods). The WNK kinases are modified by CUL3WT-KLHL3, with the higher molecular weight smear observed in anti-WNK1 and anti-FLAG panels representative of multiple ubiquitin molecules being covalently attached to the WNK protein. CUL3Δ403-459 is unable to modify WNKs. Samples from the same assay reactions were divided to allow immunodetection of the different protein components modified within the same assay reaction. (C) CUL3WT, CUL3Δ403-459 and an equimolar solution CUL3WT:CUL3Δ403-459 (1:1 Mix) were incubated with KLHL3 and immunoprecipitatedFLAG-WNK4 in ubiquitylation reactions to determine the influence of CUL3Δ403-459 on the ubiquitylation activity of CUL3WT. Notably, the presence of CUL3Δ403-459 does not inhibit WNK ubiquitylation by CUL3WT.In vitro ubiquitylation assays with purified proteins. As ubiquitin is covalently attached to the substrate lysine residue, the appearance of higher molecular weight protein bands reflects the modification of the protein with ubiquitin. All assays contain purified UBE1, UBE2D3, ubiquitin, 0.1 mM ATP, 1 mM MgCl2 and were buffered in 50 mM HEPES, 150 mM NaCl and incubated at 30°C for the time indicated. Reactions were stopped by the addition of SDS-Laemmli buffer to a concentration of 1×. SDS-PAGE, staining with Coomassie blue, or detection with the indicated antibody following immunoblotting enabled the visualisation of ubiquitylation."
NCBI gene IDs:
"CUL3: 8452"
Figure legend: "Western blot of whole kidney lysates from mice culled after a minimum 4-h fast. Following exsanguination after surgery, mouse tissues were rapidly harvested and stored, samples were then homogenised, clarified and quantified prior to SDS-PAGE. Immunodetection with the antibodies shown highlights elevated signalling through the WNK kinase pathway in CUL3WT/Δ403-459 versus CUL3WT mice. The lowest panel is an anti-CUL3 immunoprecipitation of the kidney lysate samples, whereby the CUL3 antibody was cross-linked to Protein G sepharose and used to affinity purify CUL3WT and CUL3Δ403-459 from kidney lysates. The samples were incubated together overnight to allow deneddylation of CUL3 proteins to occur and maximise CUL3 binding to the anti-CUL3 resin. Samples were thoroughly washed and eluted from the resin prior to SDS-PAGE and immunodetection for CUL3. The IP highlights that CUL3Δ403-459 is indeed present within the kidney lysate."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Plasma aldosterone after a minimum 4-h fast was calculated by HTRF (homogeneous time-resolved fluorescence) aldosterone assay. The average aldosterone level per mouse was calculated from duplicate samples run in parallel on the assay. Blood was rapidly harvested in heparin-coated plasma extraction tubes following exsanguination after surgery, and samples were snap-frozen for storage. A 58% increase in aldosterone was detected in CUL3WT/Δ403-459 versus CUL3WT mice (*P = 0.0245). Two-tailed unpaired Student's t-test; data are mean ± SEM."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Arterial blood biochemistries after a minimum 4-h fast. Under anaesthesia, the right carotid artery was cannulated to minimise atmospheric exposure of samples collected for iSTATbloodgas and electrolyte measurements. CUL3WT/Δ403-459mice present with abnormal electrolyte homoeostasis compared to CUL3WTmice, exhibiting hyperkalaemia (***P = 0.0004) and hyperchloraemia (***P = 9.5 × 10−5) with a compensated metabolic acidosis (P = 0.7766), marked by a decrease in bicarbonate () (***P = 3.4 × 10−5), base excess (BE) (***P = 9.1 × 10−5) and partial pressure of carbon dioxide (pCO2) (***P = 0.0038). Two-tailed unpaired Student's t-test; data are mean ± SEM."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Representative pseudocoloured maximum-intensity z projections of immunofluorescence stained kidney sections showing the distribution of the WNK/SPAK pathway components between CUL3WT/Δ403-459 and CUL3WTmice at a minimum 4-h fasting baseline (n = 4 per genotype). CUL3 and KLHL3 are comparable between genotypes, with significantly higher levels of CUL3 in the proximal convoluted tubule (PCT) compared to the weak staining in distal convoluted tubule (DCT) and thick ascending limb of the loop of Henle (TAL), while KLHL3 shows higher expression in the DCT/TAL cytosol with staining of the PCT confined to the apical membrane. Total (t) and phospho (p) NCC Thr44 and SPAK Thr243 show increased apical membrane expression in the parvalbumin (PVALB)-positive early and late (PVALB-negative) DCT of CUL3WT/Δ403-459mice. Unexpectedly, the increased levels of WNK4 and SPAK resulted in the formation of discrete puncta specifically in the DCT of CUL3WT/Δ403-459mice. It is possible that the autophagy-lysosomal system may attempt to compensate and degrade these excess proteins although the WNK4 puncta do not colocalise with the lysosomal marker LAMP1. Scale bar, 20 μm."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Continuous blood pressure measurements were taken in mice under general anaesthesia with thermostatically controlled body temperature, right carotid artery pressure transducer catheterisation and ECG Lead II heart rate monitoring. CUL3WT/Δ403-459 mice have elevated systolic (SBP) (***P = 1 × 10−12) and diastolic (DBP) blood pressure (***P = 8.5 × 10−8), although they also present with 7% lower heart rates (*P = 0.0485) when compared to CUL3WT mice. Two-tailed unpaired Student's t-test; data are mean ± SEM."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Pulse waveform analysis of blood pressure traces obtained in (A) reveals that CUL3WT/Δ403-459 mice have an increased pulse pressure (PP) [SBP - DBP] (***P = 2.1 × 10−10), augmentation pressure (AP) [SBP - anacrotic notch (AN) pressure] (***P = 3.5 × 10−6), dicrotic notch (DN) pressure (***P = 4.7 × 10−13) and mean arterial pressure (MAP) [1/3 × SBP + 2/3 × DBP] (***P = 1.3 × 10−9). This hypertensive phenotype is in part due to changes in vascular contractility in CUL3WT/Δ403-459 mice as evidenced by their higher augmentation index (AIx) [AP/PP] (**P = 0.0096), a marker of arterial stiffness, and is further supported by an increase in their diastolic pressure decay time constant (τbourgeois) [1/slope of diastolic pressure decay; measured 30 ms after DN and 20 ms before end DBP] (**P = 0.0083), a surrogate marker of increased vascular resistance. Two-tailed unpaired Student's t-test; data are mean ± SEM"
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "In vivo dose-responses to phenylephrine and angiotensin II. After baseline measurements were obtained from mice in (A), the right external jugular vein was cannulated for administration of bolus doses of vasopressors in increasing half-log steps. Dose-response curves were generated and data analysed using a logistical function to determine the estimated dose producing half-maximal response (ED50) and maximum response (Emax). The fitted Emax for phenylephrine was increased in CUL3WT/Δ403-459 versus CUL3WT (183.9 ± 2.5 versus 164.9 ± 1.4 mmHg) (***P = 1 × 10−6) indicating an increased vasoconstrictor response to adrenergic stimulation elevating systolic blood pressure substantially above the CUL3WT maximum. Similarly, the fitted Emax for angiotensin II stimulation was higher in CUL3WT/Δ403-459 versus CUL3WT (155.5 ± 1.8 versus 138.3 ± 2.2 mmHg) (***P = 1.7 × 10−6). However, there was no change between CUL3WT/Δ403-459 versus CUL3WT sensitivity (as measured by ED50) to phenylephrine (36.6 ± 5.1 versus 31.4 ± 2.8 μg/kg bw) (P = 0.3778) or angiotensin II (0.77 ± 0.16 versus 0.93 ± 0.13 μg/kg bw) (P = 0.4401). Two-tailed unpaired Student's t-test; data are mean ± SEM."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Western blot of tunica media-intimathoracic aorta lysates from mice culled after a minimum 4-h fast. Following exsanguination after surgery, mouse tissues were rapidly harvested and the tunica adventitia removed before storage, and later, the samples were homogenised, clarified and quantified prior to SDS-PAGE. Western blot analysis confirmed the expression of KLHL3 and CUL3. Similar to the kidney, the aorta of CUL3WT/Δ403-459 showed slightly lower levels of CUL3 compared to CUL3WT without any change in KLHL3 levels."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Representative maximum-intensity z projections of immunofluorescence stained thoracic aorta sections showing the distribution of CUL3 and KLHL3 between CUL3WT/Δ403-459 and CUL3WT mice at a minimum 4-h fasting baseline (n = 4 per genotype). CUL3 and KLHL3 localisation is comparable between genotypes. The highest levels were detected in the vascular smooth muscle cells and endothelium, with a minimal expression in the perivascular adipose tissue of the adventitia. Scale bar, 50 μm."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "Morphometric analysis of thoracic aortae reveals vascular remodelling in CUL3WT/Δ403-459 mice. There is an increase of ˜21% in the vessel wall intima-media thickness (demarcated by arrows) of CUL3WT/Δ403-459 compared to CUL3WT mice (***P = 0.0003). However, there is no change in the number of elastin laminae (P = 0.1458) and therefore no increase in the number of medial muscle layers between genotypes. Two-tailed unpaired Student's t-test; data are mean ± SEM."
NCBI gene IDs:
"CUL3: 26554"
Figure legend: "A qPCR  analysis of relative SCTR  levels in different tissues [heart  (He), intestine  (Int),  inguinal WAT (Ing), epididymal WAT (Ep), brown fat adipose  (BAT), liver  (Liv), hypothalamus  (Hypo), placenta  (Pla), skeletal muscle (Mus), kidney  (Kid)] from C57BL/6J mice  as indicated (n = 5 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"SCTR: 319229"
Figure legend: "B Change of  body weight  of M-SCT  floxed and M-SCT  KO mice  upon HFD  feeding (n = 8 per group). Right part shows representative photograph of M-SCT  floxed and M-SCT  KO mice  with HFD. Data information: Statistical significance was determined using Two-tailed Student's t test."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "C The weight  of different tissues from M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "D Random blood glucose  levels in M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 8 per group). E Insulin  levels in M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "F Intraperitoneal glucose tolerance test  (GTT) (1.0 g glucose/ kg body weight) in M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 5 per group) and AUCs were calculated. Mice  were fasted for 18h before assay. G Intraperitoneal insulin tolerance test (ITT) (0.75 U insulin/kg body weight) in M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 5 per group) and AUCs were calculated. mice  were fasted for 6h before assays. Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "H Serum TG, TC  and NEFA  levels in M-SCT  floxed and M-SCT  KO mice  with HFD  (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "A, B Time-resolved oxygen  consumption (A) and heat production  (B) in 48-hour light/dark  cycle measured by CLAMS  in M-SCT floxed and M-SCT  KO mice  with HFD  (n = 6 per group), dark  phase is marked as dark background. Regression-based absolute oxygen  consumption  (A) or  heat production (B) analysis of  body weight  are shown in the right part. Data information: Statistical significance was determined using Two-tailed Student's t test. Data in panel A and B have been analyzed using ANCOVA with oxygen consumption or heat production as dependent variable, group as fixed variable and body weight as covariate. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "C Body temperature  of 8-week-old M-SCT  floxed and M-SCT  KO male offspring with chow diet during cold  exposure (4℃; n = 8). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "D Representative HE staining  and UCP1 staining  of Ing  from M-SCT  floxed and M-SCT  KO mice  with chow diet maintained at RT  or 3 days of cold  exposure (4℃). Scale bar, 100 μm."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "E Relative mRNA levels of UCP1, CIDEA, PRDM16, PGC1α  and DIO2  in iWAT  from M-SCT  floxed and M-SCT  KO mice  with chow diet (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"CIDEA: 12683
DIO2: 13371
PGC1α: 19017
PRDM16: 70673
SCT: 319229
UCP1: 22227"
Figure legend: "F Representative western blots  of UCP1, PRDM16, PGC1α and HSP90  in iWAT  from M-SCT  floxed and M-SCT  KO mice  with chow diet  after 3 days of cold  exposure (4℃). G Total UCP1  protein per mg iWAT  of M-SCT  floxed and M-SCT KO mice  with chow diet  maintained at RT  or exposed to cold  for 3 d (4℃) (n = 3). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "H Relative mRNA levels of UCP1, PGC1α, PRDM16, CIDEA  and DIO2  in SVF  isolated from M-SCT  KO and M-SCT  floxed mice with HFD (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"CIDEA: 12683
DIO2: 13371
PGC1α: 19017
PRDM16: 70673
SCT: 319229
UCP1: 22227"
Figure legend: "I, J OCR  of Oligomycin, FCCP  and Antimycin A/Rotenone-treated matured adipocytes  derived from iWAT-SVF  of M-SCT  KO and M-SCT floxed mice  with HFD, and the AUC of OCR  as indicated were calculated (n = 5 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "K Glucose  oxidation in adipocytes  derived from iWAT-SVF  of M-SCT  KO and M-SCT  floxed mice  with HFD  (n = 8 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "B Stacked bars showing the Proportional distribution of hypo- and hypermethylated  DMRs  (M-SCT  KO vs M-SCT  floxed) in different regions. Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "C Heat map of DMR methylation  level in mRNA between M-SCT  floxed and M-SCT  KO. The color bar indicates the normalized tag counts in M-SCT  floxed or M-SCT  KO. Positive z scores, shown in the table, indicate hypermethylated regions, while negative z scores imply hypomethylated regions. D As in (C), for enhancer region."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "E Scatter plot representation of the most significant difference of methylation  in mRNA  (left) and enhancer  (right) regions between M-SCT  floxed and M-SCT  KO (p &lt; 0.005). Data information: Statistical significance was determined using Two-tailed Student's t test."
NCBI gene IDs:
"SCT: 319229"