EMBO/SourceData huggingface dataset:
Figure legend: "A Effect of KDM6A knockdown on the expression of nephrin and WT-1 in immortalized mouse podocytes cultured under high glucose conditions. Western blot analyses were performed using protein lysates of podocytes that were transfected with scrambled siRNAs or KDM6A-specific siRNAs, and cultured in high glucose (HG) or high mannitol (Mann) for 48 hours. *P &lt; 0.05 versus normal controls, #P &lt; 0.05 versus control siRNA with HG incubation (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "B Effect of KDM6A overexpression on expression levels of nephrin and WT-1. Immortalized podocytes were transfected with the KDM6A expression plasmid or the empty vector control for 48 hours, and the expression levels of KDM6A, nephrin and WT-1 in these transfected podoytes were analyzed by immunoblotting. *P &lt; 0.05 versus vector controls (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "C Changes in H3K27me3 levels in podocytes after treatment with high glucose, the combination of high glucose and KDM6A knockdown, or KDM6A overexpression for 48 hours. *P &lt; 0.05 versus normal controls, #P &lt; 0.05 versus control siRNA with HG incubation (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "D Changes in DNA methylation at nephrin gene promoter by high glucose, by the combination of high glucose plus KDM6A knockdown, or by KDM6A overexpression in immortalized podocytes. U, unmethylated-specific primers; M, methylated-specific primers. Experiments were repeated three times and a representative gel from one experiment is shown."
NCBI gene IDs:
"KDM6A: 22289
nephrin: 54631"
Figure legend: "F Changes in DNA methylation at nephrin gene promoter by GSK-J4 (40 μM) in immortalized podocytes. U, unmethylated-specific primers; M, methylated-specific primers."
NCBI gene IDs:
"nephrin: 54631"
Figure legend: "Relative mRNA levels of KDM6A, nephrin and WT-1, normalized to β-actin, expressed in kidney glomeruli of normal and diabetic mice at 4, 8 and 12 weeks after diabetic induction. *Significant differences (P &lt; 0.05) compared with normal controls (Wilcoxon two-sample test; n =8 each)."
NCBI gene IDs:
"β-actin: 
KDM6A: 22289
nephrin: 54631
WT-1: 22431"
Figure legend: "A Quantitative RT-PCR evaluation of KDM6A mRNA expression in kidney glomeruli isolated from wild-type and KDM6A-knockout (KO) mice. *P &lt; 0.05, significant difference versus wild-type controls (Wilcoxon two-sample test; n = 8)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "B Western blot analysis of KDM6A expression in glomeruli and podocytes isolated from wild-type and KDM6A-KO mice. Presented experiments were performed at least three times independently."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "C Changes in body weights and levels of blood glucose in wild-type or KDM6A-KO mice with or without STZ treatment. No significant differences in body weights or blood glucose levels between wild-type and KDM6A-KO mice with diabetes were observed during the 8-week experimental period (Wilcoxon two-sample test; n = 8)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "D Levels of urinary protein excretion, weights of kidney and levels of HbA1c in wild-type and KDM6A-KO mice with or without STZ treatment (at 8 weeks after the onset of diabetes). The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 8)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "E Immunofluorescence images of kidney sections stained with KDM6A and nephrin in wild-type (WT-NC), KDM6A-KO (KO-NC), STZ-treated wild-type (WT-DM), or STZ-treated KDM6A-KO (KO-DM) mice. Scale bars, 20 μm. *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "F Immunofluorescence images of kidney sections stained with KDM6A and H3K27me3 in wild-type (WT-NC), KDM6A-KO (KO-NC), STZ-treated wild-type (WT-DM), or STZ-treated KDM6A-KO (KO-DM) mice. Scale bars, 20 μm. *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "I Effect of high glucose on the expression of podocyte-related markers in primary cultured podocytes isolated from wild-type or KDM6A-KO mice. The primary podocytes isolated from wild-type or KDM6A-KO mice were cultured in normal or high glucose (30 mM) for 48 hours. Protein lysates from the cultured podocytes were subjected to Western blot analysis with the indicated antibodies. *P &lt; 0.05 versus wild-type podocytes in normal glucose, #P &lt; 0.05 versus wild-type podocytes in high glucose (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "A Screening of the potential KDM6A-regulated transcriptional factors involved in repression of nephrin expression. After transduction with a control lentiviral vector or a lentiviral vector expressing KDM6A into primary podocytes for 48 hours, intracellular RNAs were isolated and used for RNA sequencing (RNA-Seq) analysis. The transcript expression patterns of 86 selected transcriptional factors from three independent RNA-Seq experiments are presented in a heat map. Notably, among these 86 selected genes, KLF10 is the most upregulated gene."
NCBI gene IDs:
"KDM6A: 22289
KLF10: 21847"
Figure legend: "B Validation of increased KDM6A and KLF10 expression in primary podocytes that were infected with an empty lentiviral vector or KDM6A-expressing lentiviral vector for 48 hours. *P &lt; 0.05 versus the empty vector control (Wilcoxon two-sample test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "D Effect of KLF10 knockdown on high glucose-mediated reduction of nephrin in primary podocytes. As noted, knockdown of KLF10 prevented downregulation of nephrin and upregulation of KDM6A in high glucose-treated podocytes. *P &lt; 0.05 versus normal controls, #P &lt; 0.05 versus control siRNA with HG incubation (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "F Immunofluorescence images of KLF10 and nephrin in renal sections of wild-type or KDM6A-KO mice with or without STZ treatment. Scale bars, 20 μm. *P &lt; 0.05 versus the untreated wild-type group, #P &lt; 0.05 versus the STZ-treated wild-type group (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "H Western blot analysis of KDM6A, KLF10 and nephrin expression in primary podocytes isolated from wild-type or KDM6A-KO mice with or without STZ treatment. *P &lt; 0.05 versus the untreated wild-type group, #P &lt; 0.05 versus the STZ-treated wild-type group (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KDM6A: 22289"
Figure legend: "ChIP analysis of KLF10, acetyl-Histone H4 (H4-Ac), Dnmt1 and Dnmt3 binding to nephrin gene promoter. ChIP assays were carried out using cross-linked chromatin from primary podocytes that were cultured in normal or high glucose conditions. *P &lt; 0.05, significant difference versus the normal control group (Wilcoxon two-sample test; n = 3)."
NCBI gene IDs:
"nephrin: 54631"
Figure legend: "J Modulation of podocyte-specific marker expression in primary podocytes by KLF10 overexpression. Ectopic overexpression of KLF10 in primary podocytes significantly repressed various podocyte-specific markers, but conversely increased KDM6A expression. *P &lt; 0.05 versus the empty vector control (Wilcoxon two-sample test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "A Quantitative RT-PCR evaluation of KLF10 mRNA expression in kidney samples of wild-type and KLF10-knockout (KO) mice. **P &lt; 0.0001, significant difference versus wild-type controls (Wilcoxon two-sample test; n = 8)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "B Western blot analysis of KLF10 expression in kidney samples of wild-type or KLF10-KO mice treated with or without STZ at 8 weeks after the onset of diabetes."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "C Changes in body weight and blood glucose levels in wild-type or KLF10-KO mice with or without STZ treatment. No significant differences in body weights or blood glucose levels between diabetic wild-type mice and diabetic KLF10-KO mice were detected (Wilcoxon two-sample test; n = 8)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "D Levels of urinary protein excretion, weights of kidney and levels of HbA1c in wild-type or KLF10-KO mice with or without STZ treatment. The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 8)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "E Immunofluorescence images of kidney sections stained with KLF10 and nephrin in wild-type or KLF10-KO mice untreated or treated with STZ. Scale bars, 20 μm. *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "F Immunofluorescence images of kidney sections stained with KLF10 and KDM6A in wild-type or KLF10-KO mice untreated or treated with STZ. Scale bars, 20 μm. *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "G Immunofluorescence staining of F-actin and KLF10 in primary podocytes isolated from the wild-type or KLF10-KO mice untreated or treated with STZ. Presented experiments were performed at least three times independently. Scale bars, 20 μm."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "H Immunofluorescence staining of F-actin and KDM6A in primary podocytes isolated from the wild-type or KLF10-KO mice untreated or treated with STZ. Presented experiments were performed at least three times independently. Scale bars, 20 μm."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "I Western blot analysis of KLF10, KDM6A, nephrin and WT-1 expression in primary podocytes isolated from the wild-type or KLF10-KO mice that were untreated or treated with STZ. *P &lt; 0.05 versus untreated wild-type controls, #P &lt; 0.05 versus STZ-treated wild-type mice (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "J Western blot analysis of KLF10, KDM6A, nephrin and WT-1 expressed in podocytes treated with TGF-β1 or the combination of TGF-β1 and KLF10 knockdown. *P &lt; 0.05 versus normal controls, #P &lt; 0.05 versus control siRNA with TGF-β1 treatment (Parametric ANOVA and a Bonferroni post hoc test; n = 3)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "C Levels of KDM6A and KLF10, nephrin mRNAs in urine exosomes of diabetic nephropathy patients and non-diabetic controls. Relative mRNA levels in human urinary exosomes were normalized to 18S rRNA. Horizontal lines are medians. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 by Wilcoxon two-sample test (n = 12 for each group)."
NCBI gene IDs:
"18S rRNA: 
KDM6A: 7403
KLF10: 7071
nephrin: 4868"
Figure legend: "(C) HelaWT, HeLaHexaKO, HeLaLC3TKO, HeLaGABATKO stably expressing HT-LC3B were starved with EBSS for 90min or 6h. Cell were incubated with ± HaloTag ligand fluorescently labeled with TMR. In-gel fluoresce (TMR) and Western blots (anti-HaloTag antibody) of freed HaloTagTMR were imaged and band intensity quantified. (D) Quantification of in-gel fluorescence band intensity and Western blot with anti-Halo antibody."
NCBI gene IDs:
"GABA: 11337
LC3: 81631///440738///84557"
Figure legend: "(C) SolVit complementation/rescue analysis (quantification) of acceptor HeLaLC3TKO-HT-LC3B PNS with donor PNS from HeLaWT, HeLaLC3TKO or HelaGABATKO cells ±ATP, 1 h, 37˚C. (D) SolVit complementation/rescue analysis (quantification) of acceptor HeLaGABATKO-HT-LC3B PNS with donor PNS from HeLaWT, HeLaLC3TKO and HelaGABATKO cells ±ATP, 1 h, 37˚C."
NCBI gene IDs:
"GABA: 11337
LC3: 440738///81631///84557"
Figure legend: "(G) Co-IP analyses in HEK293T cells expressing FLAG-VPS37AFL, FLAG-VPS37AdeltaN (1-90), VPS37AdeltaN(1-20) and FLAG-Nter-EGFP and co-transfected with GFP-GABA. 5% of input was loaded. (H) Co-IP analyses in HEK293T cells expressing FLAG-VPS37AFL, FLAG-VPS37AdeltaN (1-90), VPS37AdeltaN(1-20) and FLAG-Nter-EGFP and co-transfected with GFP-LC3A. 5% of input was loaded."
NCBI gene IDs:
"EGFP: 
FLAG: 
GFP: 
GABA: 11337
LC3A: 84557
VPS37A: 137492"
Figure legend: "(I) Complementation of VPS37A KO with VPS37A constructs that have or lack mATG8-binding region. Quantification of MIL+ puncta in Huh7WT HT-LC3B (circles) and Huh7VPS37AKO HT-LC3B cells (squares), transfected with empty FLAG, full length VPS37A (FLAG-VPS37AFL) or mutant VPS37A (FLAG-VPS37AdeltaN(1-20)). Data information: Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparison test. All values are means ± SD, n = 3 biologically independent replicates, each HCM experiment: 1,000 valid primary objects/well, 6 wells/sample. (J) HCM images, examples corresponding to Fig 5I, scale bar, 10 μm."
NCBI gene IDs:
"FLAG: 
VPS37A: 137492"
Figure legend: "(B) MPL-MIL HCM assay in Huh7WT and Huh7VPS37AKO stably expressing HT-LC3B. Quantification: (B i) membrane-permeant HT ligand (MPL) staining of LC3B-II sequestered within sealed membranes. (B ii) MIL staining of membrane-bound HT-LC3B-II accessible to the cytosol and (B iii) MIL/MPL puncta ratio."
NCBI gene IDs:
"VPS37A: 137492"
Figure legend: "(F-H) MPL-MIL HCM assay in Huh7WT (control or CHMP2A siRNA-treated cells; immunoblot in F) stably expressing HT-LC3B during CCCP-induced mitophagy in ± BafA1 (100 nM). (G) Quantification: (G i) MPL+ sealed membranes; (G ii) MIL+ unsealed membranes; (G iii) MIL/MPL puncta ratio. (H) HCM images corresponding to panel G."
NCBI gene IDs:
"CHMP2A: 27243"
Figure legend: "(C) Quantification of MIL+ puncta (unsealed/ligand-accessible LC3B+ membranes) in Huh7WT (circles) or Huh7VPS37AKO (squares) cells stably expressing HT-LC3B. Cells were transfected with empty FLAG vector, FLAG-VPS37A full length (VPS37AFL), FLAG-VPS37AdeltaN(1-90), or FLAG-VPS37AdeltaN(1-20) constructs. CCCP, 6 h treatment. (D) HCM images of Huh7VPS37AKO HT-LC3B with VPS37A constructs (VPS37AFull, FLAG-VPS37AdeltaN(1-90), FLAG-VPS37AdeltaN(1-20) and FLAG-VPS37ANter-GFP). FLAG-Tag VPS37A constructs, expression detected as red; and MIL+ puncta, pseudo color green. Data information: Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparison test. All values are mean ± SD, n = 3 biologically independent experiments, each HCM experiment: 1,000 valid primary objects/cells per well, 6 wells/sample, Scale bar, 10μm. "
NCBI gene IDs:
"FLAG: 
GFP: 
VPS37A: 137492"
Figure legend: "Preadipocytes  bearing the shRNA targeting  Pgc1α were induced differentiation with or without 1mM  asparagine. Protein expression was analyzed on day 6. n=3 biological replicates."
NCBI gene IDs:
"Pgc1α: 19017"
Figure legend: "Brown preadipocytes were infected by lentiviral shRNAs against  Asns. Cells were differentiated in culture media with or without 1mM asparagine. Western blot  was performed on day 6."
NCBI gene IDs:
"Asns: 27053"
Figure legend: "Brown preadipocytes were knocked down Raptor following standard induction to mature adipocytes with or without asparagine supplementation. Protein expression analysis was performed on day 6."
NCBI gene IDs:
"Raptor: 74370"
Figure legend: "(A) A431 WT and SKO cells -/+ GFP-fused WT or A212P-seipin were delipidated, treated with OA for 1 h, fixed and stained with LD540. Maximum intensity projections of deconvolved confocal z-stacks. Dashed lines indicate cell boundaries. (B) Analysis of LD areas of cells treated as in (A) and imaged with widefield microscopy. Bars: % of LDs/cell in indicated size category, mean ± SEM, n=57-61 cells/genotype, 2 experiments, p**&lt; 0.005 (unpaired T-test)."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(C) SKO + WT- or A212P-seipin-GFP cells were stained for calreticulin. Maximum intensity projections of deconvolved confocal z-stacks."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(D) SKO + WT- or A212P-seipin-GFP cells were grown in standard growth medium, fixed and stained with anti-GFP antibodies. Widefield and STORM images."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(E) SKO + WT-seipin-GFP cells were delipidated and treated with OA for 1h, fixed and stained with LipidTox Red. Single focal plane of a 3D-SIM z-stack. (F) Analysis of (E), bars: % of LDs/cell with the indicated number of WT-seipin-GFP punctae associated, mean ± SEM, n=12 cells (693 LDs), 2 experiments, p**&lt; 0.005 (Tukey multiple comparisons test)."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(G) SKO + WT- or A212P-seipin-GFP cells were delipidated, incubated with OA for 30 min, and immunolabelled for TEM with anti-GFP antibodies. Black arrowheads: immuno-gold, orange arrowheads: ER-LD junctions. See also Appendix Fig S3. (H) Analysis of (G), bars: relative labeling index in different cellular compartments, n=3456-4512 immuno-gold clusters. Distribution of both WT- and A212P-GFP-seipin immunolabel is non-random (chi square test, **p&lt;0.005), but there is significant enrichment of WT- but not A212P-seipin-GFP at ER-LD contacts relative to the ER (binomial test, **p&lt;0.005). See also Appendix Table S1 and S2."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(I) WT and SKO cells stably expressing HSP47-APEX were delipidated, treated with OA for 1 h and processed for SB-EM. 3D models of LDs and surrounding ER and mitochondria profiles shown on top of a block face image from SB-EM dataset. Insets are representative cross sections from modeled area highlighting the ER-LD association. Red= LD, Yellow= ER, Green=mitochondria. (J) Analysis of LDs from (I). Black lines indicate median, boxes third quartiles, n= 98-227 LDs. (K) Analysis of LDs displaying no ER contacts from (I). Bars: mean % of LDs in ROIs not in proximity to the ER, ± SEM, n= 11-16 ROIs/genotype, **p&lt;0.005 (unpaired T-test)."
NCBI gene IDs:
"HSP47: 871"
Figure legend: "(A) A431 WT cells with seipin tagged at its chromosomal locus with sfGFP and stably expressing ER marker plasmid (BFP-KDEL) were delipidated and treated with OA for 20 h, fixed and stained with LipidTox Red. Single confocal plane of an airyscan z-stack."
NCBI gene IDs:
"seipin: 26580"
Figure legend: "(A) Two control and BSCL2fibroblast cell lines were delipidated and treated with OA for 1 h, fixed and stained with LD540. Maximum intensity projections of deconvolved confocal z-stacks."
NCBI gene IDs:
"BSCL2: 26580"
Figure legend: "(B) Control and BSCL2fibroblasts were incubated with OA for 75-90 min, LDs were stained with LD540 and cells imaged live with widefield microscopy. Insets show overlay of two subsequent frames (400 ms apart). (C) Analysis of (B). Bars = 1 - [Pearson's colocalization coefficient between subsequent frames/ROI], ± SEM, n=120-140 ROIs (60-70 cells), 2 experiments, **p&lt;0.005 (Mann Whitney test)."
NCBI gene IDs:
"BSCL2: 26580"
Figure legend: "(D) Control and BSCL2fibroblasts were transfected with ER marker plasmid (sfGFP-ER-3) for 24 h and treated with OA for 10-60 min. LipidTox Deep Red or LD540 was used to label LDs and cells were imaged live by airyscan microscopy. Examples of LDs with high mobility are shown, blue arrowheads indicate a LD moving along the ER, orange arrowheads indicate LDs moving independent of the ER. (E) Analysis of ER associated movement of LDs from live airyscan microscopy as in (D). Bars: % of LDs/ROI displaying indicated mode of movement, mean ± SEM, n= 50-42 ROIs (218-374 LDs), 2 experiments, **p&lt;0.005 (Mann Whitney test)."
NCBI gene IDs:
"BSLC2: 26580"
Figure legend: "(F) Control and BSCL2fibroblasts were treated with OA for 1 h, fixed and stained with anti-ACSL3 antibodies and LD540. Representative sections of peripheral ER areas, maximum intensity projections of deconvolved confocal z-stacks. (G) Analysis of (F), with insets illustrating assigned categories of ACSL3 LD association. Insets are the same as in Fig 4F. Bars: % of LDs/ROI showing the category indicated, mean ± SEM, n= 11-10 peripheral ROIs from 11-10 cells/group (1200-2019 LDs), 2 experiments, **p&lt;0.005 (unpaired T-test)."
NCBI gene IDs:
"BSCL2: 26580"
Figure legend: "C Hsf1(1-408) with a deleted TAD is dissociated from HSE-DNA by Hsc70, DnaJB1 and ATP with similar rates as Hsf1wt. Fluorescence polarization assays containing Alexa Fluor® 488-HSE-DNA bound Hsf1(1-408) trimers and the indicated components."
NCBI gene IDs:
"Hsf1: 3297"
Figure legend: "A The rate of Hsc70/DnaJB1-mediated dissociation of HSE-DNA bound Hsf1 depends on the number of HR-B proximal Hsc70 binding sites in the Hsf1 trimer. Hsf1wt (wt) and Hsf1∆(202-213) (∆) monomers were mixed in the indicated ratios and heat shocked at 42°C for 10 min to form mixtures of different homo- and heterotrimers as indicated with the cartoons (the red line and the arrow head indicate the Hsc70 binding site). Numbers indicate the relative fraction of the different species."
NCBI gene IDs:
"Hsf1: 3297"
Figure legend: "B Moving the Hsc70 binding site away from the trimerization domain reduces the rate of Hsc70/DnaJB1-mediated dissociation of HSE-DNA-bound Hsf1. Hsf1_t10/20/30, region 202-213 moved by 10, 20, or 30 residues towards the C-terminus."
NCBI gene IDs:
"Hsf1: 3297"
Figure legend: "C Anti-FLAG antibodies are not able to dissociate HSE-DNA-bound Hsf1. Red lines in the cartoon indicate the Hsc70 binding site; blue lines and arrow head indicate the inserted FLAG epitope DYKDDDDK. Hsf1_i201/211/221, FLAG-epitope inserted after residue 201, 211 or 221. Anti-FLAG antibodies were split in halfmers by incubation with 2 mM DTT"
NCBI gene IDs:
"FLAG: 
Hsf1: 3297"
Figure legend: "Anti-FLAG antibody halfmers bound to FLAG-epitope containing Hsf1 variants. Hsf1wt and FLAG-insertion variants were analyzed by blue-native gel electrophoresis in the absence or presence of DTT-treated anti-FLAG antibodies as indicated and Hsf1 detected by immunoblotting; t-bound, FLAG antibody-bound Hsf1 trimers; m-bound, FLAG antibody bound Hsf1 monomer."
NCBI gene IDs:
"FLAG: 
Hsf1: 3297"
Figure legend: "F Trimer-to-monomer ratio of freshly purified Hsf1wt and Hsf1-I190S,I194S in the absence of heat shock, determined by gel filtration and BN-Gel"
NCBI gene IDs:
"Hsf1: 3297"
Figure legend: "G Rate of Hsc70/DnaJB1-mediated dissociation of HSE-DNA bound Hsf1wt and Hsf1-I190S,I194S"
NCBI gene IDs:
"Hsf1: 3297"
Figure legend: "A Schematics of the used system. MEFs, in which HSF1 on chromosome 8 was deleted, were stably transfected with mTag-BFP expressed under the control of the HSPA6 promoter and subsequently transiently transfected with plasmids that expressed HSF1wt or mutant variants in an artificial operon with hrGFP, which is translated from an IRES element. B Exemplary flow cytometry data plotting BFP fluorescence versus GFP fluorescence, indicating the gates used for analysis. Numbers indicate the fraction (%) of cells (forward scatter singlets) in each gate. Color indicates the cell density from dark blue (low) to red (high)."
NCBI gene IDs:
"BFP: 
GFP: 
mTag: 
HSF1: 15499
HSF1: 3297
HSPA6: 3310"