EMBO/SourceData huggingface dataset:
Figure legend: "(D) Quantification of futsch mRNA levels obtained from isolated 3rd instar larvae brains of the indicated genotypes via real-time PCR analysis. Bars show average ± SD of biological triplicates. Bars are labelled with the number of replicates."
NCBI gene IDs:
"futsch: 5740544"
Figure legend: "E-G) Representative Western blot analysis of the protein level (E) and quantification of the translation efficiency of S2R+ cells transfected with the wildtype or mutated futsch reporter constructs upon control or Mettl3 KD (F) or Ythdf KD (G). LacZ dsRNA was used for the control KD. The translation efficiency was calculated as the ratio of the relative GFP protein level and GFP mRNA level. mCherry served as a normalization control for both the protein and mRNA level. Bars show mean ± s.e.m of biological triplicates. P values were determined with a Student's t-test. (n.s. = not significant; p&lt;0.05 = *; p&lt;0.01 = **)."
NCBI gene IDs:
"LacZ: 
futsch: 5740544
Mettl3: 42844
Ythdf: 42995"
Figure legend: "(b) Decreasing autophagy levels by deletion of the Atg5 gene (left) or depletion of ATG7 by siRNA (right) significantly increases the percentage of MBd+ cells (P=0.0019 and P=0.021, respectively, n=3). Immunoblots confirm loss of the Atg5-Atg12 conjugation in mutant cells and depletion of ATG7 (asterisk). GAPDH, glyceraldehyde 3-phosphate dehydrogenase."
NCBI gene IDs:
"Atg5: 11793
ATG7: 10533"
Figure legend: "(c) Rapamycin (Rapa) and LiCl co-treatment induces autophagy and decreases the percentage of MBd+ cells (left, HeLa; P=0.0056, n=3). Immunoblots showing increased LC3-II levels confirm autophagy induction. Induction of autophagy by overexpression of Flag-tagged BECN1 reduces the percentage of MBd+ cells (right, MCF-7; P=0.0008, n=4). Ctrl, control."
NCBI gene IDs:
"BECN1: 8678"
Figure legend: "(a) Single-plane confocal microscopy images showing co-localization of the MBds and the autophagic receptor NBR1 in U2OS cells and p62-deleted MEFs. MBd markers: MKLP1 or Cep55. Scale bar, 2 μm."
NCBI gene IDs:
"p62: 18412"
Figure legend: "(b) The percentage of MBd+ cells is significantly increased following the depletion of NBR1 (P=0.022, n=3), but not another autophagic receptor, p62. Co-depletion of NBR1 and p62 does not further increase MBd levels over NBR1 depletion alone."
NCBI gene IDs:
"NBR1: Q14596///4077"
Figure legend: "(c) Deletion of the p62 gene does not affect the percentage of MBd+ cells. For b and c, immunoblots verify protein loss."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(a-c) Reprogramming is more efficient after MBd enrichment. Differentiated cells (dH1f) and embryonic fibroblasts (IMR90) are reprogrammed after stable expression of either NBR1 shRNA or shNT. Emerging iPSC colonies are scored on the basis of TRA-1-60 expression. Cells depleted of NBR1 to increase MBd levels show an increase in iPSC colony formation (a,b, dH1f, 3.1±0.5-fold, n=15, P=0.00035; IMR90, 3.4±0.8-fold, n=3, P=0.02; data are mean±s.e.m.) but insignificant changes in autophagic activity (c) over shNT control. (b) Representative plates with TRA-1-60-immunostained iPSC colonies. The immunoblot (c, top) and densitometry (c, bottom; percentage of autophagic flux) show representative results (n=3); α-tubulin, loading control."
NCBI gene IDs:
"NBR1: 4077"
Figure legend: "(e,f) MBd enrichment in cancer cells leads to increased anchorage-independent growth. MKLP1-GFP-expressing HeLa cells are separated into 'MBd-high' and 'MBd-low' subpopulations. An increase in the 'MBd high' over 'MBd low' ratio is associated with an increase in soft-agar colony formation (e). No significant difference was observed when the enrichment of the MBd-high subpopulation was less than threefold. More soft-agar colonies are formed when MBds are enriched by NBR1 depletion (NBR1 shRNA) in HeLa (f, left; P=0.0012, n=3) and mouse 134-4 cells (f, right; P=0.0086, n=3); control, shNT. Data are mean±s.d., and the colony number (e,f) is the sum of INT-violet-stained colonies from ten random fields."
NCBI gene IDs:
"NBR1: 17966
NBR1: 4077"
Figure legend: "Quantitative RT-PCR analysis for IFN-β mRNA (B) levels in wild type BMMs 5 and 24 hr post-treatment with EVs respectively (1 μg/ml equals approximately 3 x 108 vesicles/ml). Data shown are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"IFN-β: 15977"
Figure legend: "(D) IFN-β mRNA level was quantified in wild type BMMs treated with EVs at a concentration of 10μg/ml for the times indicated. Data shown are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"IFN-β: 15977"
Figure legend: "Quantitative RT-PCR analysis for IFN-β mRNA (E) levels in either WT, MAVS- and/or RIG-I-knockdown BMMs treated for 4 hr (E) with 10µg/ml EVs from uninfected or M.tb-infected macrophages. (Control) negative control siRNA. Data shown are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"RIG-I: 230073
IFN-β: 15977
MAVS: 228607"
Figure legend: "IFN-β protein (F) levels in either WT, MAVS- and/or RIG-I-knockdown BMMs treated for 24 hr (F) with 10µg/ml EVs from uninfected or M.tb-infected macrophages. (Control) negative control siRNA. Data shown are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"RIG-I: 230073
MAVS: 228607"
Figure legend: "(G) Similar to above except using WT and Mavs -/- BMMs. Data shown are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "(H) Similar to above except using WT and Mavs -/- BMMs. Scale bar, 20 μM. Data shown in (H) are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "(J) Top chamber: WT BMMs; Bottom chamber: WT or Mavs -/- BMMs. Data shown in (J) are the mean ± SD (n = 3 wells per group) and all data shown are representative of three independent experiments (biological replicates). * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "(K) Western blot analysis for phospho-TBK1 (Ser172) in WT and MAVS-knockdown BMMs treated for 4 hr with EVs from uninfected or M.tb-infected macrophages. β-actin served as a loading control. Densitometry of the western blots for (K) are shown."
NCBI gene IDs:
"MAVS: 228607"
Figure legend: "(N) IRF3 nuclear translocation was analyzed in RIG-I- or MAVS-knockdown BMMs. Scale bar, 20 μM."
NCBI gene IDs:
"RIG-I: 230073
MAVS: 228607"
Figure legend: "(B) EVs from cells infected with WT, ∆esxA or esxA-complemented (∆esxA-C) M.tb Erdman strains."
NCBI gene IDs:
"esxA: 886209"
Figure legend: "(D) EVs from cells infected with WT, ∆esxA or esxA-complemented (∆esxA-C) M.tb Erdman strains. Data shown are the mean ± SD (n = 3 per group) and all data shown are representative of three independent experiments."
NCBI gene IDs:
"esxA: 886209"
Figure legend: "qRT-PCR analysis for IFN-β mRNA (G) in WT BMMs treated with EVs for 4 hr and 24 hr respectively. The EVs were isolated from BMMs that were infected with the different M.tb CDC 1551 strains. Data shown are the mean ± SD (n = 3 per group) and all data shown are representative of three independent experiments. * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"IFN-β: 15977"
Figure legend: "qRT-PCR analysis for IFN-β mRNA (J) in WT BMMs treated with EVs for 4 and 24 hr respectively. The EVs were isolated from BMMs that were infected with the different M.tb Erdman strains. Data shown are the mean ± SD (n = 3 per group) and all data shown are representative of three independent experiments."
NCBI gene IDs:
"IFN-β: 15977"
Figure legend: "Immunofluorescence microscopy analysis for colocalization of M.tb (GFP) with lysosome marker Lamp-1 in Mavs-/- (E and F) mouse BMMs. Cells were pre-treated for 5 hr with EVs from uninfected or M.tb-infected macrophages plus IFN-γ, and then infected with GFP-expressing M.tb for 24 hr prior to immunostaining. quantitative analysis of M.tb colocalization with Lamp-1 in Mavs-/- mouse BMMs Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Immunofluorescence microscopy as described above except using RIG-I siRNA-treated (I and J) BMMs. quantitative analysis of M.tb colocalization with Lamp-1 in RIG-I siRNA-treated mouse BMMs Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"RIG-I: 230073"
Figure legend: "M.tb CFU in infected Mavs-/- (K) mouse BMMs pre-treated with IFN-ɣ plus EVs from uninfected (EVs_Control) or M.tb-infected (EVs_M.tb) BMMs. CFU was determined immediately after the 1 hr infection or 24 and 72 hr post-infection. Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. ; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "M.tb CFU in control siRNA-treated (L) or RIG-I siRNA-treated (M) mouse BMMs pre-treated with IFN-ɣ plus EVs from uninfected (EVs_Control) or M.tb-infected (EVs_M.tb) BMMs. CFU was determined immediately after the 1 hr infection or 24 and 72 hr post-infection. Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"RIG-I: 230073"
Figure legend: "(A) Immunofluorescence microscopy analysis for colocalization of M.tb with Lamp-1 in WT BMMs pre-treated with EVs from macrophages infected with WT, ∆secA2 or secA2-complemented (∆secA2-C) M.tb CDC 1551 strains. The cells were pre-treated with EVs supplemented with recombinant mouse IFN-γ for 5 hr and subsequently infected for 24 hr with GFP-expressing M.tb. (B) quantitative analysis for the colocalization of M.tb with Lamp-1. Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM ; * p &lt; 0.05 and ** p &lt; 0.01 by two-tailed Student's t-test."
NCBI gene IDs:
"secA2: 885594"
Figure legend: "(C) M.tb CFU in WT BMMs pre-pretreated with recombinant mouse IFN-γ and EVs from macrophages infected with WT, ∆secA2 or secA2-complemented (∆secA2-C) M.tb CDC 1551 strains. Data shown are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. n.s., not significant; * p &lt; 0.05 and ** p &lt; 0.01 by two-tailed Student's t-test."
NCBI gene IDs:
"secA2: 885594"
Figure legend: "Immunofluorescence microscopy and quantitative analysis for colocalization of M.tb with autophagosome marker LC3 (C and D) in RIG-I siRNA-treated BMMs. Quantitative data are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM. n.s., not significant; * p &lt; 0.05 , ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"RIG-I: 230073"
Figure legend: "Immunofluorescence microscopy and quantitative analysis for colocalization of M.tb with markers LC3 in Mavs -/- BMMs. Quantitative data are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM. n.s., not significant; * p &lt; 0.05 , ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Immunofluorescence microscopy quantitative analysis for colocalization of M.tb similar to ( I -L), but using Mavs -/- BMMs. NOX2 (M and N) Quantitative data are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM. n.s., not significant; * p &lt; 0.05 , ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Immunofluorescence microscopy quantitative analysis for colocalization of M.tb similar to ( I -L), but using Mavs -/- BMMs. p47phox (O and P) Quantitative data are the mean ± SD of three independent infections and all data shown are representative of at least three independent experiments. Scale bar, 5 μM. n.s., not significant; * p &lt; 0.05 , ** p &lt; 0.01 and *** p &lt; 0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "(A and B) Quantitative analysis of immunofluorescence microscopy images for colocalization of M.tb with Lamp-1 in WT (A) and Mavs -/- (B) BMMs infected with M.tb for 24 hr and then treated for an additional 24 hr with moxifloxacin (MFX) and/or EVs from M.tb-infected BMMs (EVs+MFX) in the presence of recombinant mouse IFN-ɣ. Mock, no EVs or moxifloxacin treatment; EVs_Control, EVs from uninfected BMMs. Data shown are representative of three independent infections. The results are the mean ± SD of three independent experiments. n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Mavs -/- BMMs were used and colocalization of M.tb with LC3 (F) were quantified. Data shown are representative of three independent infections. The results are the mean ± SD of three independent experiments. n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Mavs -/- BMMs were used and colocalization of M.tb with NOX2 (G) were quantified. Data shown are representative of three independent infections. The results are the mean ± SD of three independent experiments. n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Mavs -/- BMMs were used and colocalization of M.tb with p47phox (H) were quantified. Data shown are representative of three independent infections. The results are the mean ± SD of three independent experiments. n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "(I and J) M.tb CFU analysis in WT (I) and Mavs -/- (J) BMMs infected with M.tb for 24 hr followed by treatment with EVs, moxifloxacin or combination in the presence of IFN-ɣ for 24 and 72 hr. Data shown are representative of three independent infections. The results are the mean ± SD of three independent experiments. n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "Representative histopathological analysis for lung sections of Mavs -/- (D) mice that were infected with M.tb and subsequent left untreated (Mock) or treated with EVs from uninfected BMMs (EVs_Control), EVs from M.tb-infected BMMs (EVs), moxifloxacin (MXF), or combination of EVs and MXF (EVs+MXF). Data shown is representative of two independent experiments. The results are the mean ± SD (n = 4 mice per group). Scale bar, 100 μM"
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "M.tb CFU in the lung and spleen of Mavs -/- (E) mice treated with EVs, MXF or combination of both. Data shown is representative of two independent experiments. The results are the mean ± SD (n = 4 mice per group). n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "ELISA analysis for, IFN-β, TNF-α, IL-1β and IFN-γ protein level in serum isolate from M.tb-infected WT (F) or Mavs -/- (G) mice treated with EVs, MXF or a combination of both. Data shown is representative of two independent experiments. The results are the mean ± SD (n = 4 mice per group). n.s., not significant; * p &lt; 0.05, ** p &lt; 0.01 and *** p &lt; 0.001 by Student's t-test (two tailed)."
NCBI gene IDs:
"Mavs: 228607"
Figure legend: "A Proteins captured by BGL3 and RMRP antisense purifications. Proteins with an absolute log2 (fold change) &gt; 1 and -log10 (p-value) &gt; 1.3 (compared with control purification) were considered as proteins enriched by BGL3 specifically."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "C&amp;D RNA pull-down assay for BGL3-BARD1/PARP1 interactions before and after irradiation. Biotinylated in vitro-transcribed LncRNA-BGL3 sense or antisense transcripts were incubated with HEK-293T cell lysates with or without ionizing radiation (10 Gy) with 1 hr recovery for in vitro streptavidin RNA pull-down assays, followed by Western blots using the indicated antibodies."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "HA-tagged full-length or deletion mutants of BARD1 were transfected into 293T cells. 48 hr later, BGL3 RNA pull-down assay were performed, and arrowheads indicate the bands for HA-tagged full-length or deletion mutants of BARD1"
NCBI gene IDs:
"HA: 
BARD1: 580
BGL3: 103344929"
Figure legend: "HA-tagged full-length or deletion mutants of PARP1(H) were transfected into 293T cells. 48 hr later, BGL3 RNA pull-down assay were performed,"
NCBI gene IDs:
"HA: 
BGL3: 103344929
PARP1: 142"
Figure legend: "A BGL3 is recruited to DNA damage sites. U2OS cells were subjected to laser micro-irradiation to generate DSBs in a line pattern. The relocation kinetics of BGL3 to DSBs was monitored by RNA fluorescence in situ hybridization (FISH) in a time course as indicated. BGL3 intensities at the laser line were normalized into a numerical value using Nikon NIS-Elements AR software (version 4.40.00). Data are presented as mean ±SD of four biological replicates."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "B Chromosome aberrations induced by BGL3 depletion. Wild-type or BGL3 depleted BJ-5ta cells were used in the metaphase spread analysis for spontaneous DNA breaks. Representative image (left panel) and the percentage of cells containing at least one DNA break (right panel). Data are presented as mean ± SD of three biological replicates. Two-tailed Student's t test, ** P&lt;0.01."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "C MCF-7 cells were treated as indicated, and cell response to ionizing radiation were analyzed by colony formation assays (left panel). Data are presented as mean ± SEM of four independent experiments, analyzed by two-way analysis of variance (ANOVA), **P &lt; 0.01. Knock-down efficiency of BGL3 siRNA was examined by qRT-PCR and normalized to β-Actin (middle panel), data are presented as mean ± SD of three biological replicates. Two-tailed Student's t test, **P &lt; 0.01. Knock-down efficiency of CtIP siRNA was examined by blotting (right panel)."
NCBI gene IDs:
"BGL3: 103344929
CtIP: 5932"
Figure legend: "E BGL3 deficiency inhibits DNA damage repair. Quantification of γ-H2AX foci at indicated times after irradiation (2Gy) are presented. Data shown are results of three independent experiments (100 cells for each experiment), presented as mean ± SD, two-tailed Student's t test, *P &lt; 0.05, **P &lt; 0.01, NS: no significant difference."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "C-F Wild-type (CTRL) or BGL3 knockdown U2OS cells were subjected to micro-irradiation; 1 hr later, cells were fixed and processed for immunostaining with indicated antibodies. Representative images of BRCA1 (C), BARD1 (D), RAD51 (E), and RPA70 (F) accumulation at sites of laser-induced DNA damage are shown."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "Parental or BGL3-deficient U2OS cells were subjected to laser micro-irradiation to generate DSBs in a line pattern. DDR factor recruitment were examined 2 hr later. Data shown are the average of three independent experiments, and 100 cells were counted for each experiment. Data are presented as mean ± SD, analyzed by two-tailed Student's t test, **P &lt; 0.01."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "E U2OS cells were transfected with the indicated siRNA, and RAD51 accumulation at sites of laser-induced DNA damage were examined. Data shown are the average of three independent experiments, and 100 cells were counted for each experiment. Data are presented as mean ± SD, analyzed by two-tailed Student's t test, NS: no significant difference. Knock-down efficiency of BARD1 siRNA was examined by blotting (bottom left panel), and knock-down efficiency of BGL3 siRNA was examined by qRT-PCR and normalized to β-actin (bottom right panel), data are presented as mean ± SD of three biological replicates. Two-tailed Student's t test, **P &lt; 0.01."
NCBI gene IDs:
"β-actin: 
BARD1: 580
BGL3: 103344929"
Figure legend: "A BGL3 promotes BARD1-HP1γ interactions. 293T cells expressing the indicated siRNAs were irradiated (10 Gy), and BARD1-HP1γ interactions examined at the indicated time points."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "B BGL3 promotes RAP80 binding to K63 ubiquitin chain in cells. 293T cells expressing the indicated siRNAs and plasmids were irradiated (10 Gy). Cell lysates were immunoprecipitated with HA beads and subjected to immunoblot with the indicated antibodies."
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "C BGL3 promotes BARD1-HP1γ (up panel) and BARD1-RAD51 (middle panel) interactions in vitro. GST-HP1γ, GST-RAD51, and FLAG-BARD1 were overexpressed and purified from cells as indicated in Methods. FLAG-BARD1 was divided into two equal parts, one part was used for BARD1-HP1γ interaction analysis (up panel), and another part was used for BARD1-RAD51 interaction analysis (middle panel). BARD1-HP1γ (up panel) and BARD1-RAD51 (middle panel) interactions with or without BGL3 were respectively detected by GST pull-down assays, here GST was used as a pull-down control."
NCBI gene IDs:
"FLAG: 
GST: 
BGL3: 103344929"
Figure legend: "A PARP1 inhibitor treatment abolished BGL3 recruitment to DSBs. U2OS cells were treated with DMSO, PARP inhibitor (olaparib), ATM inhibitor (KU-55933), ATR inhibitor (VE-821) or caffeine. BGL3 recruitment to DNA damage sites was assessed by RNA-FISH assays. B Quantification of the positive cells. For each group, 100 randomly selected cells were counted. Data are presented as mean ±SD of three biological replicates. Two-tailed Student's t test, **P &lt; 0.01, N: no significant difference. "
NCBI gene IDs:
"BGL3: 103344929"
Figure legend: "C PARP inhibitor mainly affected BGL3 recruitment at an early time point. U2OS cells were treated with DMSO or olaparib, and BGL3 recruitment was examined following laser micro-irradiation at indicated time point. Data presented are the average of three independent experiments, and 100 cells were counted for each experiment. Two-tailed Student's t test, **P &lt; 0.01, N: no significant difference."
NCBI gene IDs:
"BGL3: 103344929"