EMBO/SourceData huggingface dataset:
Figure legend: "(F) Formation of puncta in primary B cells complemented with AID-FUS-GFP  or AID-FUSm-GFP. FUSm contains 13 mutations on either Y or R residue. Left: Representative images showing cellular localization  of AID-FUS-GFP  and AID-FUSm-GFP  with and without CRM1  inhibitor (CRM1i) treatment in Aicda-/- primary B cells. Scale bar, 5 µm. Right: Quantification of the number of cells with condensed puncta (3 biological replicates). Unpaired two-tailed Student's t-test was performed. ****, p&lt;0.0001."
NCBI gene IDs:
"GFP: 
Aicda: 11628
AID: 11628
FUS: 233908"
Figure legend: "(G) CSR  levels to IgG1   in activated Aicda-/- primary B cells complemented with the indicated protein. Left, representative flow cytometry plots measuring CSR  to IgG1. Co-expressed GFP  was used to indicate the infected fractions. Right, the mean with SD of three biological replicates is shown in the bar plot. Unpaired two-tailed Student's t-test was performed. ****, p&lt;0.0001."
NCBI gene IDs:
"Aicda: 11628"
Figure legend: "(A) Representative immunofluorescence  images of endogenous AID  in the presence of ectopically-expressed EV, AID, AIDR190X, or AIDcry in cytokine-stimulated CH12F3  cells. EV, empty vector control. An antibody against AID   C terminus (185-198 aa) detected both endogenous and ectopically-expressed full-length AID. The nucleolus  is indicated by co-nucleofected mCherry-FBL. The white dashed line depicts the shape of the nucleus  based on Hoechst staining. Arrows indicate the location  of puncta formed by endogenous AID. The images were taken by the N-SIM microscope and processed via N-SIM software. A middle slice of the images was shown. Scale bar, 5 µm."
NCBI gene IDs:
"AID: 11628"
Figure legend: "(B)  Co-condensation  of AID  and AIDR190X in the nucleus  of CH12F3  cells in the early G1 phase. Top: experimental layout. GFP-tagged wild-type AID  (green) and mCherry-tagged AID  variants (red) were co-nucleofected in Aicda-/- CH12F3  cells. The resulting cells were arrested with nocodazole  for 12 hours after transfection. Six hours later, nocodazole  was removed from the system for 1.5 hours before imaging. Bottom left: representative images showing subcellular localization  of indicated AID  variants. The white dashed line depicts the shape of the nucleus  based on Hoechst staining. The white arrow indicates the location  of the co-condensed puncta. Scale bar, 5 µm. Bottom right: plot profiles of the images. Cyto: cytoplasm, Nuc: nucleus."
NCBI gene IDs:
"GFP: 
mCherry: 
Aicda: 11628
AID: 11628"
Figure legend: "(D) Dynamics of trapping of wild-type AID  in optoAIDR190X condensates. Top, representative fluorescence images at different time points. Dashed circles indicate the co-condensed  puncta formation of AID-GFP. Scale bar, 5 µm. Bottom, a summary of 12 observed cells is shown as mean ±SD in a bar graph. Percentages of  optoAIDR190X puncta with trapped wild-type AID  are plotted for the second and third blue-light activations, and the colored dot indicates one cell."
NCBI gene IDs:
"AID: 11628"
Figure legend: "(B) The optoDroplet  formation of indicated AID  mutants along with light  stimulation. Top, the procedure of optoDroplet  assay is illustrated with indicated light  stimulation (blue arrow) and image acquisition (black arrow) time points. Bottom, line plots show quantification of cells with optoDroplet  formation as mean ± SD at each acquisition  time point. The total acquired cell numbers of each genotype are indicated in parentheses. Significance assessment between AIDR190X and AIDR190X-4R was shown at each  time point. Unpaired two-tailed Student's t-test was performed. ****, p&lt;0.0001."
NCBI gene IDs:
"AID: 11628"
Figure legend: "(C) CSR  levels of ectopically-expressed AID variants in AID-proficient CH12F3  cells. Left, representative flow cytometry plots of CSR  to IgA. Co-expressed GFP  indicates the infected cell fractions. Right, the CSR  mean with SD of three biological replicates is shown in the bar plot. (D) CSR  levels of ectopically-expressed AID   variants in CSR-activated B cells. Left, representative flow cytometry plots of CSR  to IgG1. Right, the bar plot shows the CSR  mean with SD of four biological replicates. Data information: One-way ANOVA followed by Dunnett's multiple comparisons test was performed for (C) and (D). ***, p&lt;0.001; **, p&lt;0.01; *, p&lt;0.05."
NCBI gene IDs:
"AID: 11628"
Figure legend: "(B) CSR  to IgA  induced by AID  variants in CH12F3  cells. Left, representative  flow cytometry plots. Right, the CSR  mean with SD of three biological replicates is shown in the bar plot. One-way ANOVA followed by Dunnett's multiple comparisons test was performed. ***, p&lt;0.001; **, p&lt;0.01."
NCBI gene IDs:
"AID: 11628"
Figure legend: "A. Anterior and lateral photographs of two patients  with ELP6  variants showing dysmorphic features at 20 (Patient  2) and 19 (Patient  3) years of age."
NCBI gene IDs:
"ELP6: 54859"
Figure legend: "B. Mid-sagittal (I), coronal  (II) and transversal  (III) MRI  scans of Patient  1 with newly identified ELP4  variants and Patient  2 and Patient  3 with ELP6  variants. Atrophy  of the cerebellum  with prominence of the cerebellar folia is marked by circles. Arrows indicate thin corpus callosum, red arrowheads point to enlarged extra-axial CSF  spaces and black arrowheads to cerebral sulci in keeping with cerebral  and cerebellar  volume loss."
NCBI gene IDs:
"ELP4: 26610
ELP6: 54859"
Figure legend: "D. Co-immunoprecipitation  analyses of Elongator complexes in patient-derived fibroblast  cell. Whole cell lysates (left) and eluates after  immune-precipitation (right) are shown for control fibroblasts  (ctrl) and cultured patient fibroblasts  (Elp4Y91C/L296I (patient  1); Elp6L118W/L118W (patient  2),  Elp6L118W/L118W (patient  3). Used antibodies for immobilization (ELP1) and detection (ELP1, ELP3, ELP4  , ELP5  and α-tubulin) are indicated. n = 4 independent experiments."
NCBI gene IDs:
"Elp4: 26610
Elp6: 54859"
Figure legend: "F. Differential expression  (DE) of peptides in fibroblasts  obtained from the individuals with ELP4  and ELP6  variants relative to control (n = 3 technical repeats per genotype). Down- (red) and upregulated (blue) peptides are depicted on volcano plots."
NCBI gene IDs:
"ELP4: 26610
ELP6: 54859"
Figure legend: "The relative abundance of CggR (normalized to the abundance measured on glucose and the same promoter) is almost constant with PTEFmut7 across multiple growth rates in WT and TM6 cells, but not with PTEFmut2 and PCMV. The CggR intracellular levels were quantified by proteomics in steady-state cultures grown in minimal media with glucose, galactose, maltose or pyruvate as carbon sources at a final concentration of 10 gL-1. WT data includes all three promoters, whereas TM6 only includes the PTEFmut2 and PTEFmut7 data. Error bars represent the standard deviation of at least three replicate experiments."
NCBI gene IDs:
"CMV: 
TEF: 856195"
Figure legend: "The four reporter plasmids were transferred to the wildtype strain containing the CggR (R250A) under the control of the PTEFmut7. The strength of the four promoters was assessed by quantifying YFP (FL1) and mCherry (FL3) fluorescence in exponentially growing wildtype cells in minimal medium with 10 g l-1 glucose. The FL1 and FL3 fluorescence shown is the non-background corrected median of 100 000 cell events. The non-FL control is the signal from a wildtype strain grown under the same conditions. The background fluorescence, assessed by the wildtype harboring the YCplac33 plasmid, was substracted from FL1 and FL3. The final reporter activity is the ratio of the background-corrected YFP and mCherry values. Error bars represent the standard deviation of three independently determined ratios from three replicate experiments."
NCBI gene IDs:
"mCherry: 
YFP: 
CggR: 938570
TEF: 856195"
Figure legend: "Reporter activity of the sensor across (D) multiple FBP levels Reporter activity is given by the YFP/mCherry ratio, calculated through the quantification of YFP and mCherry fluorescence along culture time using flow cytometry. Both YFP and mCherry fluorescence levels were first corrected for background using the same strains harboring the YCplac33 plasmid (Appendix Table S8). The control is the wildtype and TM6 strains expressing only the reporter plasmid without CggR. Error bars represent the standard deviation of at least three replicate experiments."
NCBI gene IDs:
"mCherry: 
YFP: 
CggR: 938570"
Figure legend: "Reporter activity of the sensor across glycolytic fluxes. The glycolytic flux is reported as the flux between the metabolites fructose 6-phosphate (F6P) and fructose-1,6-bisphosphate (FBP). Glycolytic fluxes were here estimated on the basis of physiological and metabolome data and a novel method to estimate intracellular fluxes (Niebel et al, 2019). Reporter activity is given by the YFP/mCherry ratio, calculated through the quantification of YFP and mCherry fluorescence along culture time using flow cytometry. Both YFP and mCherry fluorescence levels were first corrected for background using the same strains harboring the YCplac33 plasmid (Appendix Table S8). The control is the wildtype and TM6 strains expressing only the reporter plasmid without CggR. Error bars represent the standard deviation of at least three replicate experiments."
NCBI gene IDs:
"mCherry: 
YFP: 
CggR: 938570"
Figure legend: "Sub-populations of WT and TM6 cells, grown separately and mixed in different fractions as indicated in percentages, can easily be distinguished by flow cytometry, FL1 YFP channel, FL3 mCherry channel. Histogram of single-cell ratios of FL1/FL3 fluorescence intensities of mixed WT and TM6 populations analyzed by flow cytometry. Here, a sub-population of minimally 5% can be distinguished."
NCBI gene IDs:
"mCherry: 
YFP: "
Figure legend: "Tukey boxplots showing the YFP/mCherry ratio of individual cells measured by microscopy as a function of glycolytic flux. At least 35 cells were analyzed in each condition. The glycolytic flux is here reported as the flux between the metabolites fructose 6-phosphate (F6P) and FBP. Glycolytic fluxes were estimated on the basis of physiological and metabolome data and a novel method to estimate intracellular fluxes (Niebel et al, 2019)."
NCBI gene IDs:
"mCherry: 
YFP: "
Figure legend: "YFP/mCherry ratio measured by microscopy in co-existing dividing (high-flux) versus non-dividing (low-flux) isogenic TM6 cells on 10 gL-1 glucose."
NCBI gene IDs:
"mCherry: 
YFP: "
Figure legend: "The production rates of YFP and mCherry are uncoupled during the cell cycle in the biosensor-expressing strain (F), which reflects the cell-cycle dynamics of intracellular FBP concentration and glycolytic flux. In a control strain, lacking CggR, the production rates of YFP and mCherry are coupled (G). The uncoupling was calculated for individual cell-cycle trajectories as the difference between the YFP and mCherry production rates normalised to have the same scale (see more details in Materials and methods; Appendix Figure S10). Each curve represents the mean across the indicated number of cell cycles in a replicate experiment. The corresponding shaded areas denote the 95% confidence intervals of the means (bootstrapping with 5 000 iterations). We smoothed the single-cell-cycle trajectories of YFP and mCherry signals as well as cell volume via the Gaussian process regression, and used these trajectories to derive the YFP and mCherry production rates, accounting for fluorescent-protein maturation in a first-order kinetics model. To align the cell-cycle trajectories and to calculate the phase, we used the array of three cell cycle events E {cytokinesis (cyt), budding, next cyt} as reference points. The cell cycles used for the analysis had the duration in the interval between 150 and 300 minutes, with the mean duration presented in parentheses for each replicate experiment. The cells belonged to the TM6 strain and were cultivated on 20 gL-1 glucose in the microfluidic device."
NCBI gene IDs:
"mCherry: 
YFP: 
CggR: 938570"
Figure legend: "Bisulfite sequence analysis of DMRs of the imprinted genes (PEG1, KvDMR1, PEG10, and NESP55) in iPSCs (top) and d6 TRA‐1‐81+/cKIT+ PGCLCs (bottom). White and black circles represent unmethylated and methylated CpG sequences, respectively."
NCBI gene IDs:
"NESP55: 2778
KvDMR1: 10984
PEG1: 4232
PEG10: 23089"
Figure legend: "Effect of knockdown of BLIMP1 and PRDM14 on PGCLC induction. Knockdown of BLIMP1 (F) and PRDM14 (G) was done using the lentiviral system with two individual shRNA vectors for each gene. The knockdown efficiencies were assessed by qPCR (left panel, each). The induction of d4 PGCLCs analyzed by FACS, gated for TRA‐1‐81 and cKIT (right panel, each). Data are presented as means ± SD (n = 3)."
NCBI gene IDs:
"BLIMP1: 639
PRDM14: 63978"
Figure legend: "Spleen, mLN, sdLN, liver, adipose, and lung lymphocytes from Jα18-/- or Vα14 mice were stimulated in vitro with RAW-CD1d cells and 1μg α-GalCer. An additional sample of Vα14 lymphocytes from each organ was plated with RAW-CD1d cells but no α-GalCer. Supernatants were collected after 24 hours and cytokine concentration determined by cytokine bead array. Error bars are SD of mean values from three different mice per group. Results shown are representative of two independent experiments where n=3 biological replicates."
NCBI gene IDs:
"CD1d: 12479///12480
Jα18: 100124371
Vα14: 100126466"
Figure legend: "Lymphocytes from the indicated tissues of C57BL/6 and Vα14 mice were stained with anti-CD3 and CD1d (PBS57) tetramer, before they were fixed, permeabilized, and stained with antibodies to T-bet, RORγt, and PLZF. Results shown are gated on CD3+CD1d-tetramer+ cells."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Lymphocytes from the Peyer's patches of C57BL/6 and Vα14 mice were stained with anti-CD3 and CD1d (PBS57) tetramer. Percentage of lymphocytes that were CD3+CD1d-tetramer+ iNKT cells among the Peyer's patches of C57BL/6, Vα14, and Jα18-/- mice are shown. Mann-Whitney test. Error bars are SD. C57BL/6 n=22; Vα14 n=16; Jα18-/- n=8."
NCBI gene IDs:
"Jα18: 100124371
Vα14: 100126466"
Figure legend: "(C-E)Mice were analyzed for total fecal IgA by ELISA. Mann-Whitney test. Error bars are SD. C57BL/6 n=14; Vα14 n=11; Jα18-/- n=13."
NCBI gene IDs:
"Jα18: 100124371
Vα14: 100126466"
Figure legend: "(C-E)Mice were analyzed for total fecal IgG1 (D) or total serum IgG1 (E) by ELISA. Mann-Whitney test. Error bars are SD. C57BL/6 n=14; Vα14 n=11; Jα18-/- n=13."
NCBI gene IDs:
"Jα18: 100124371
Vα14: 100126466"
Figure legend: "Percentages of total B cells that were IgG1+ in the spleen, mLN, and Peyer's patches of C57BL/6, Vα14, and Jα18-/- mice are shown. Mann-Whitney test. Error bars are SD. C57BL/6 n=15; Vα14 n=15; Jα18-/- n=5"
NCBI gene IDs:
"Jα18: 100124371
Vα14: 100126466"
Figure legend: "CD1d (PBS57) tetramer+ CD3+ cells were sorted from spleens or PP of 3 different Vα14 TN mice along with CD4+CD3+CD1d tetramer- cells from PP (PP CD4). RNAseq was performed. Heatmap of FPKM values for the indicated Tfh genes across each RNAseq sample."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Spleen, mLN, and PP lymphocytes from Vα14 and C57BL/6 mice were stimulated with PMA and ionomycin. Lymphocytes were stained with anti-CD3, CD1d (PBS57) tetramer, anti-IL-4, and anti-IFNγ. Percentages of iNKT cells and non-iNKT T cells within the population of CD3+IL-4+ cells and CD3+IFNγ+ cells, n = 5 mice for C57BL/6 group and n = 4 mice for Vα14 group. Error bars are SD."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Vα14 TN mice were administered α-GalCer either 2μg intravenously or 5μg by oral gavage. Mice were given Brefeldin A intraperitoneally after 30 min, and tissues were harvested 3 hours later. Cells from spleen, mesenteric lymph node, and Peyer's patches of Vα14 TN mice were permeabilized, fixed, and stained with antibodies to IL-4, IFNγ, and IL-17. Plots shown are gated on CD1d-(PBS57)-tetramer+ CD3+ iNKT cells."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Pooled spleen and LN cells from a Vα14 TN mouse were cocultured with wild type B cells with or without 1μg α-GalCer and with or without agnostic anti-CD40. IgM was measured by ELISA of culture supernatants 4 days later."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Pooled spleen and LN cells from a Vα14 TN mouse were cocultured with anti-CD40 activated wild type B cells with or without 1μg α-GalCer and with blocking antibody to CD1d (1μg/mL, clone 1B1) or isotype control as indicated. IgM, IgG1, and IgA were measured by ELISA of 4 day culture supernatants."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Spleen, mLN or PP cells from Vα14 TN iNKT mice and PP cells from an IL-4-/- mouse were cocultured with anti-CD40 activated wild type or CD1d-/- B cells. 1μg α-GalCer was added to the cultures to specifically activate iNKT cells. IL-4 were measured by ELISA of culture supernatants."
NCBI gene IDs:
"CD1d: 12480///12479
IL-4: 16189
Vα14: 100126466"
Figure legend: "Spleen, mLN or PP cells from Vα14 TN iNKT mice and PP cells from an IL-4-/- mouse were cocultured with anti-CD40 activated wild type or CD1d-/- B cells. 1μg α-GalCer was added to the cultures to specifically activate iNKT cells. Blocking antibodies to IL-4 or isotype were added as indicated. IgG1 (D-E) were measured by ELISA of culture supernatants."
NCBI gene IDs:
"CD1d: 12480///12479
IL-4: 16189
Vα14: 100126466"
Figure legend: "Spleen, mLN or PP cells from Vα14 TN iNKT mice and PP cells from an IL-4-/- mouse 1μg α-GalCer was added to the cultures to specifically activate iNKT cells. Blocking antibodies to IL-4 or isotype were added as indicated. IgG1+ and IgA+ class switched B cells were enumerated by flow cytometry after 4 days of coculture"
NCBI gene IDs:
"IL-4: 16189
Vα14: 100126466"
Figure legend: "Spleen, mLN or PP cells from Vα14 TN iNKT mice and PP cells from an IL-4-/- mouse were cocultured with anti-CD40 activated wild type or CD1d-/- B cells. 1μg α-GalCer was added to the cultures to specifically activate iNKT cells. Blocking antibodies to IL-4 or isotype were added as indicated. IgG1+ class switched B cells were enumerated by flow cytometry after 4 days of coculture"
NCBI gene IDs:
"CD1d: 12480///12479
IL-4: 16189
Vα14: 100126466"
Figure legend: "Cells from spleen, mesenteric lymph node, and Peyer's patches of Vα14 TN mice were permeabilized, fixed, and stained with the indicated antibodies. Plots shown are gated on CD1d-(PBS57)-tetramer+ CD3+ iNKT cells. Quantification of data from E, n=3 mice per group. Mann-Whitney test. Error bars are SEM. *p&lt;0.05."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "Spleen and PP cells from Vα14 TN mice were stained with the indicated antibodies, analyzed by flow cytometry and gated on CD1d (PBS57) tetramer+CD3+ cells."
NCBI gene IDs:
"Vα14: 100126466"
Figure legend: "(A) Immunoblot  showing the activation of PI3K/AKT  pathway (by assessing the levels of p-AKT Ser473) of ECs 24h after induction of Pik3caH1047R expression."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(B) Volcano plot of genes analysed by RNAseq in ECs  upon Pik3caH1047R expression. Differentially expressed genes (DEGs) (p adj &lt; 0.05) in Pik3caH1047R over Pik3caWT ECs  at 24h (grey, unchanged; orange, downregulated; purple, upregulated). n=4 biological replicates per genotype."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(D) Immunoblot  showing the activation of PI3K/AKT  pathway of  Pik3caWT and Pik3caH1047R ECs  after 2h starvation   (-) and 30 min stimulation with 20%  fetal bovine serum (FBS) (+)."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(E, F) (E)  Cell cycle analysis  by flow cytometry  of  Pik3caWT and Pik3caH1047R ECs  after cultured either without serum  (-) or 20% serum  (+) for 24h. (F) Percentage of proliferative ECs  (in S and  G2M  phases) from Fig 1E. Error bars are s.e.m. n ≥ 3 biological replicates per genotype. Statistical analysis was performed by nonparametric Mann-Whitney test. **p &lt; 0.01 and ****p &lt; 0.0001 were considered statistically significant."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(B) Representative images of control and EC-Pik3caH1047R/WT mouse retinas  isolated at indicated  time points stained  with IB4  for blood vessels. Scale bars: 150 μm."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(A, B) Representative images of EC-Pik3caH1047R/WT (A) and EC-mTmG (B) P6 retinas from mice  treated with decreasing doses of 4-OHT on P1. Retinas  were stained  for  blood vessels  (IB4) and GFP  as indicated. Scale bars: 150 μm."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(D) Quantification of IB4-positive area per retina  in Pik3caH1047R/WT retinas. Data presented as a percentage of the control for each  4-OHT dose. Error bars are s.e.m. n = 4 retinas  per genotype. Data information: Statistical analysis was performed by nonparametric Mann-Whitney test. *p &lt; 0.05 was considered statistically significant."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(A) Representative images of EC-Pik3caH1047R/WT P6 retinas  isolated from mice  treated with 0.125 mg/kg of  4-OHT  on P1. Retinas  were immunostained  for blood vessels (IB4). Red asterisks show arteries  and arterioles  and yellow arrowheads veins. Scale bars: 150 μm (left panel) and 30 μm (right, high magnification panels). (B) Representative images of P6 retinas  from control and EC-Pik3caH1047R/WT mice  treated with 0.125 mg/kg 4-OHT on P1, following immunostaining  for p-S6  (S235/236) and blood vessels  (IB4). Scale bars: 150 μm (left panels) and 30 μm (right panels, high magnification). (C) Representative control and EC-Pik3caH1047R/WT P6 retinas immunostained  for blood vessels (IB4), EC nuclei  (Erg) and EdU. Scale bars: 150 μm (left panels) and 30 μm (right panels, high magnification). (D-G) Quantification of (D) p-S6  (S235/236) intensity (presented as a fold change of vehicle-treated control), (E) EC proliferation by EdU staining, (F) EC  number by Erg  positive cells and (G) retinal vascularity  by IB4-positive area in control and EC-Pik3caH1047R/WT retinas. Error bars are s.e.m. n &gt; 5 retinas  per genotype. Statistical analysis was performed by nonparametric Mann-Whitney test. **p &lt; 0.01 was considered statistically significant."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(B, C) Representative images of P6 retinas  isolated from control and EC-Pik3caH1047R/WT mouse  littermates. Blood vessels  were stained  with IB4. Lower panels showing high magnification images of the representative areas showing (B) blood vessels (IB4), EC nuclei  (Erg), EdU  incorporation and (C) pS6 (S235/236). Scale bars: 150 μm (upper panel) and 30 μm (lower panels). (D-G) Quantification of (D) retinal vascularity  by IB4 staining, (E) EC  number by Erg immunostaining, (F) EC proliferation  by EdU staining, and (G) pS6 (S235/236) intensity (presented as a fold change of vehicle-treated control). Bars represent the mean ± s.e.m. n ≥ 4 retinas  per genotype. Statistical analysis was performed by nonparametric Mann-Whitney test. *p &lt; 0.05 and **p &lt; 0.01 were considered statistically significant."
NCBI gene IDs:
"Pik3ca: 18706"
Figure legend: "(B, C) Representative confocal  images of P6 retinas  isolated from control and EC-Pik3caH1047R/WT mouse  littermates. Blood vessels were stained  with IB4. Lower panels showing high magnification images of the representative areas showing (B) blood vessels (IB4), EC nuclei  (Erg), EdU  incorporation and (C) pS6 (S235/236). Scale bars: 150 μm (upper panels) and 30 μm (lower panels). (D-G) Quantification of (D) retinal vascularity  by IB4 staining, (E) EC  number by Erg immunostaining, (F) EC proliferation  by EdU staining, and (G) pS6 (S235/236) intensity (presented as a fold change of vehicle-treated control). Bars represent the mean ± s.e.m. n ≥ 3 retinas  per genotype. Statistical analysis was performed by nonparametric Mann-Whitney test. *p &lt; 0.05 and **p &lt; 0.01 were considered statistically significant."
NCBI gene IDs:
"Pik3ca: 18706"