EMBO/SourceData huggingface dataset:
Figure legend: "C. Graph showing deformation of the whole parasite and its nucleus between invading and non-invading parasites. Indicated parasites were analysed. For adf cKD induced and non-induced conditions were compared, as indicated. 30 parasites were counted for each condition in triplicate. One-way ANOVA was used for statistical analysis and Tukey's multiple comparisons test. **** p value &lt;0.0001. *** p value 0.0009. Data information: Error bars represent standard deviation. ; and Scale bars represent 5 µm."
NCBI gene IDs:
"adf: 7893682"
Figure legend: "A. Time-lapse analysis depicting invading RH adfcKD parasites expressing Cb-EmeraldFP. A F-actin ring can be observed at the TJ in the majority of cases (red arrowheads, first panel). Invasion can also occur without formation of an F-actin ring (second panel). During capped invasion, an F-actin ring is formed (third panel). Invasion of adf cKD parasites occurs without formation of an F-actin ring (fourth panel). F-actin accumulation is observed at the posterior pole of the parasite in all cases (orange arrows). Green segmented lines depict the area that was measured for generation of intensity plot profiles shown in (B). Yellow and purple dotted lines indicate the extra- and intracellular part of the parasite respectively. B. Intensity plot profiles of the movies shown in (A). Depicted plots correlate to half-invaded parasites. In the case of F-actin ring formation, two distinct peaks are detected, correlating to the F-actin ring at the TJ (red arrowhead) and the posterior pole of the parasite (orange arrow). In the case of no F-actin ring, no accumulation at the TJ can be observed; only the peak correlating to posterior F-actin can be detected. For adf cKD, only one peak can be seen corresponding to the posterior pole of the parasite. C. Quantification of F-actin accumulation at the TJ for RH Cb-EmeraldFP parasites as detected in time lapse analysis. The values are expressed as percentage. 28 total parasite invasion events were captured across three different biological replicates. Two-tailed unpaired t-test analysis was performed. **** p value &lt;0.0001. Data information: Error bars represent standard deviation. White arrows point to direction of invasion. Scale bars represent 5 µm. ****p value &lt;0.0001."
NCBI gene IDs:
"EmeraldFP: 
adf: 7893682"
Figure legend: "E. Tight Junction assay on fixed samples depicting invasion rates of indicated parasites. While in the majority of control parasites F-actin can be detected at the junction (~80%), no junctional ring can be detected in ~20% of all invasion events. In contrast, adf cKD, act1 cKO or myoA KO only show an overall invasion rate of ~20% compared to controls. In these cases junctional F-actin accumulation could never be detected. Note that the number of total invasion events for adf cKD, act1cKO and myoAKO correlates to the number of invasion events, seen for RH without F-actin ring formation. Data information: Error bars represent standard deviation. One-way ANOVA analysis was performed for each graph ****p value &lt;0.0001."
NCBI gene IDs:
"act1: 7896590
adf: 7893682
myoA: 7900154"
Figure legend: "A. Time-lapse analysis of invading RH Cb-EmeraldFP parasites. During penetration, an F-actin ring is formed at the TJ (orange arrow). The nucleus (purple) is squeezed through the TJ (red arrowhead) and posterior F-actin appears to be directly connected to the nucleus and TJ (blue arrow). B. Analysis of nucleus deformation during live imaging shown in A. The nucleus constricts while its passing through the TJ (red arrowheads). C. The posterior end of the parasite deforms during the invasion process. F-actin is accumulated during invasion, with the posterior end contracting (blue arrow). Data information: Numbers indicate minutes:seconds. Scale bar represents 5 µm."
NCBI gene IDs:
"EmeraldFP: "
Figure legend: "D. Time-lapse stills depicting RH Cb-EmeraldFP parasites invading in absence of detectable F-actin at the junction (orange arrow). The nucleus (purple) is squeezed through the TJ (red arrowhead) during invasion. Actin accumulation at the posterior pole of the parasite is strongly detected in all cases (blue arrow). E. Analysis of nucleus deformation during live imaging shown in (D). The nucleus appears to not show constriction once its passing through the TJ when the F-actin ring is not present. F. The posterior end of the parasite also deforms during the invasion process."
NCBI gene IDs:
"EmeraldFP: "
Figure legend: "G. Z-stack gallery depicting mid-invading myoA KO parasites. Note that the nucleus is located located at the back during invasion events. Data information: Scale bar represents 5 µm. White arrow points to direction of invasion."
NCBI gene IDs:
"myoA: 7900154"
Figure legend: "A. SR-SIM images depicting invading parasites with and without the presence of the F-actin ring at the tight junction and invading adf cKD Cb-EmeraldFP parasites. SAG1 staining (in red) was performed prior to permeabilisation to specifically label extracellular part of the parasite. Note the accumulation of actin (blue arrow) around the nucleus and the posterior pole of the parasite, irrespective of F-actin ring formation at the TJ (orange arrowhead). 3D rendering is shown for each presented case (inlet). Data information: Scale bar represents 5 µm for SR-SIM images White arrow points to direction of invasion."
NCBI gene IDs:
"EmeraldFP: 
adf: 7893682"
Figure legend: "D. Quantification of perinuclear actin in indicated parasites. Parasites were counted by assessing Cb EmeraldFP signal as an actin marker around or in the vicinity of the nucleus. Still images to the right represent what was considered as F-actin - nucleus association. The orange arrowhead points to F-actin surrounding the nucleus in the posterior end of the parasite, the red and yellow arrowhead shows F-actin in the vicinity of the nucleus, hinting at a close interaction between the two. Three biological replicates were analysed with a minimum of 25 parasites per replicate. Data information: Error bars represent standard deviation. Scale bar represents 5 µm for SR-SIM images White arrow points to direction of invasion."
NCBI gene IDs:
"EmeraldFP: "
Figure legend: "E-G. SR-SIM images showing three stages of invading wt parasites. SiR-tubulin staining for microtubules (in red) was performed prior to fixation to specifically label microtubules in the parasite. During invasion, the microtubules (MT) are deformed at the TJ area (white arrowhead). Accumulation of actin (green arrow) at the posterior forms a F-actin meshwork. The nucleus is deformed when it enters the TJ area (orange arrowhead). H-J. In the case of the myoA KO strain, the MTs also deform when passing through the TJ (white arrowhead). However, F-actin is not concentrated at the posterior pole or the TJ, but evenly distributed within the cytosol of the parasite, with some peripheral location. K-M. In case of adfKD, MTs are deformed at the TJ area (white arrowhead). F-actin is accumulated at both ends of the parasite (green arrowheads), with no accumulation at the TJ. Data information: Scale bar represents 5 µm for SR-SIM images White arrow points to direction of invasion."
NCBI gene IDs:
"adf: 7893682
myoA: 7900154"
Figure legend: "A. Different invasion stages of P. falciparum merozoites expressing Cb-EmeraldFP. DAPI labels the nucleus (DNA), Cb-EmeraldFP labels actin filaments (CB-EME, green) and anti-RON4 labels the junction (red). Left panel: onset of invasion. Middle panel: A merozoite in the middle of invasion; note the constriction of the nucleus (blue) as it passes through the RON4 junction and F-actin colocalising with it (green). Right panel: A merozoite completing invasion; note the F-actin ring beyond the RON4-junction and actin filaments surrounding the nucleus. Data information: Scale bar represents 1 µm for Plasmodium falciparum"
NCBI gene IDs:
"EME: 
EmeraldFP: "
Figure legend: "C. Super-resolution images showing various stages of invasion of erythrocytes by P. falciparum merozoites expressing Cb-EmeraldFP. Left panel: DAPI labels the nucleus (blue), Cb-EmeraldFP labels actin filaments (CB-EME, green) and the invasion junction is marked with an anti-RON4 antibody (red). Brightfield images have been marked with red dots depicting the junction, black dots depicting merozoite boundary still outside the erythrocyte and white dots depicting a merozoite that has penetrated the host cell. Top panel shows an attached merozoite beginning the process of invasion, the second panel during the process of invasion and the bottom two panels show merozoites completing the process of invasion. Right panel: Schematic depicting merozoite invasion into red blood cells. Letters depict the corresponding event in the model (left) and microscopy pictures (right). White arrows depict actin filaments that colocalise with the nucleus. D. Quantification of Plasmodium falciparum invasion events categorising actin-TJ and actin-nucleus association events. 121 events were counted in total. Data information: Error bars represent standard deviation. Scale bar represents 1 µm for Plasmodium falciparum"
NCBI gene IDs:
"EME: 
EmeraldFP: "
Figure legend: "(A) Representative flow cytometry plots (left) and pooled data (right) of competition assays with sgControl and sgRnf31 B16-F10 ova tumor cells after culture with the indicated recombinant cytokine(s) (10 ng/mL) for 48 hours; n.s.: no significance, ****p &lt; 0.0001, Unpaired t-test for n = 3 biological replicates. Pooled data are represented as mean ± SEM."
NCBI gene IDs:
"Rnf31: 268749"
Figure legend: "(B) CRISPR/Cas9 screen showing depleted B16-F10 genes after one week culture with TNF + IFN-γ (both 10 ng/mL); blue genes indicate overall genes with p &lt; 0.001, black genes indicate genes of interest with p &lt; 0.05. Significance assessed using MAGeCK test."
NCBI gene IDs:
"CRISPR: 
Cas9: 57852564"
Figure legend: "(H) Representative flow cytometry plots (left) and pooled data (right) showing PI staining of sgControl or sgRNF31 A375 tumor cells after culture with the indicated recombinant cytokine(s) (10 ng/mL) for 48 hours; n.s.: no significance, *p &lt; 0.05, **p &lt; 0.01, Unpaired t-test for n = 3 biological replicates. Pooled data are represented as mean ± SEM."
NCBI gene IDs:
"RNF31: 55072"
Figure legend: "(A) PI staining of sgControl or sgRnf31 B16-F10 tumor cells treated with combined TNF/IFN-γ (both 10 ng/mL) for 24 hours in the absence and presence of QVD-OPh (10 μM); n.s.: no significance, ***p &lt; 0.001, ****p &lt; 0.0001, Unpaired t-test for n = 3 biological replicates. Pooled data are represented as mean ± SEM."
NCBI gene IDs:
"Rnf31: 268749"
Figure legend: "(B) Western blot analysis of the indicated proteins on whole cell lysates for sgControl or sgRnf31 B16-F10 tumor cells cultured with the indicated cytokine(s) (10 ng/mL for all) for 0, 3, 6, or 9 hours; β-actin was used as a loading control."
NCBI gene IDs:
"Rnf31: 268749"
Figure legend: "(D) CRISPR/Cas9 screen showing enriched genes from sgRnf31 B16-F10 tumor cells after successive treatment with combined TNF/IFN-γ, as described in (C); red genes indicate overall genes with p &lt; 0.0001. Significance assessed using MAGeCK test."
NCBI gene IDs:
"CRISPR: 
Cas9: 57852564
Rnf31: 268749"
Figure legend: "(G) PI staining of sgControl, sgRnf31, sgRnf31/Stat1, sgRnf31/Casp8, sgRnf31/Bid, or sgRnf31/Rela B16-F10 tumor cells treated with combined TNF/IFN-γ for 24 hours; *p &lt; 0.05, ****p &lt; 0.0001, asterisk(s) indicated group vs TNF/IFN-γ-treated sgRnf31 group, Unpaired t-test for n &gt; 3 biological replicates. Pooled data are represented as mean ± SEM."
NCBI gene IDs:
"Bid: 12122
Casp8: 12370
Rela: 19697
Rnf31: 268749
Stat1: 20846"
Figure legend: "(B) Individual (left) and average (right) in vivo growth of subcutaneous wild-type or sgRnf31 B16-F10 ova tumors left untreated or treated with adoptively transferred OT-I CD8+ T cells (on days indicated by black arrows); *p &lt; 0.05, **p &lt; 0.01, Unpaired t-test for OT-I-treated wild-type vs OT-I-treated sgRnf31 with n = 5/6 mice per group. Pooled data are represented as mean ± SEM."
NCBI gene IDs:
"Rnf31: 268749"
Figure legend: "(a) RV-infected   MA-NSP5-EGFP cells at 6 HPI. NSP5-EGFP-tagged viral factories (green) are dissolved in the presence of 4.7% (v/v) propylene glycol (PG, middle). Viral RNA-protein condensates rapidly reform (&lt;10 min) after replacing the PG-containing cell culture medium (PG removed, right). Seg3 (magenta) and Seg4 (cyan) transcripts are detected by smFISH, and colocalising Seg3 and Seg4 RNA signals (white). Scale bars: 50 µm, zoomed-in regions: 10 µm."
NCBI gene IDs:
"EGFP: 
MA: 
NSP5: 
Seg3: 
Seg4: "
Figure legend: "(e) 3D DNA-PAINT analysis of Seg3 RNA foci in RV-infected cells at 4 and 6 HPI. Scale bars, 2 µm (left) and 200 nm (zoomed-in, right). (f-g) Distribution of calculated volumes and sphericities of the Seg3  RNA-containing granules in  RV-infected cells at 4 HPI (N=704) and 6 hpi (N=698), shown in (e). Box plots represent the 25th/75th interquartile range, with whiskers representing the 5th/95th percentile values. Medians shown as central bands, and means shown as squares. Symbol 'x' denotes 1% and 99% percentile values, and min and max values are shown as '-'. At the 0,001 level, the two distributions are significantly different between 4 and 6 hpi, assessed by the two-sample Kolmogorov-Smirnov test."
NCBI gene IDs:
"Seg3: "
Figure legend: " [a] Sagittal  sections obtained from mice (p15) of the indicated genotypes were immunostained  for ADP-ribose using the pan-ADP-ribose detection reagent MABE1016. Representative  images showing levels of ADP-ribose in the hippocampal regions CA1, CA3, and dentate gyrus (DG), and in the cerebral cortex. Red dotted boxes highlight the elevated ADP-ribose staining in Xrcc1Nes-Cre cerebellum (left dotted box) and hippocampus (right doted box). Scale bars: 5 mm, 50 μm. WT (n = 4 mice), Xrcc1Nes-Cre (n = 4), Parp1+/- /Xrcc1Nes-Cre (n = 4), Parp1-/-/Xrcc1Nes-Cre (n = 3) and Parp1-/- (n = 3). Summary histograms show mean ± SEM. Pairwise comparisons between WT versus Xrcc1Nes-Cre mice and WT versus Parp1-/-/Xrcc1Nes-Cre mice  were conducted by Kruskal-Wallis ANOVA with Dunn's post-hoc test and statistically significant differences (*p&lt;0.05) are shown. "
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: " [b] Protein extracts from wild type (WT), Xrcc1Nes-Cre, and Parp1-/- forebrain tissue, containing hippocampus and cortex, were incubated with 1 mM NAD+ for 45 min in the presence or absence of PARP inhibitor as indicated, and ADP-ribosylation  detected by western blotting using the poly(ADP-ribose)-specific detection reagent MABE1031. Representative images from two or more independent experiments are shown. A western blot showing the level of Parp1 and Xrcc1  in the  forebrain tissue extracts is also shown, Inset. "
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: " [b] Video monitoring and recording of generalised running/bouncing seizures in mice of the indicated genotypes from P15-P19. The point of death of Xrcc1Nes-Cre mice  by fatal seizure is indicated (cross). Note no  seizures from mice of other genotypes were detected. WT (n = 9 mice), Xrcc1Nes-cre (n = 3 mice), Parp1+/- /Xrcc1Nes-Cre (n = 3 mice), and Parp1-/-/Xrcc1Nes-Cre  (n = 3). "
NCBI gene IDs:
"Cre: 2777477
cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: " [b] Heatplots of cumulative SLEs: WT (n=11 slices from 3 mice), Xrcc1Nes-Cre (n=12 slices from 4 mice), Parp1+/-/Xrcc1Nes-Cre (n=12  slices from 3 mice) and Parp1-/-/Xrcc1Nes-Cre  (n=10 slices from 3 mice). Each horizontal bar corresponds to one  slice with the colour code indicating cumulative SLEs. "
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: " [e] Summary histograms (mean ± SEM) of cumulative seizure-like activity at 5, 10, and 15 min timepoints in cortex, CA1, and CA3  regions of wild type and Xrcc1Nes-Cre brain slices from mice treated or not for 5-8 days ad libitum with PARP1 inhibitor (ABT-888) prior to analysis. WT (n = 8 slices from 4 mice), Xrcc1Nes-Cre (n = 10 from 5 mice), WT +  PARPi (n = 6 from 3 mice), Xrcc1Nes-Cre + PARPi  (n = 9 from 3 mice). Pairwise comparisons at the 15 min time point between WT versus Xrcc1Nes-Cre mice and WT versus Xrcc1Nes-Cre mice  +  PARPi were conducted by Kruskal-Wallis with Dunn's post-hoc tests and statistically significant differences (*p&lt;0.05) are shown. "
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Xrcc1: 22594"
Figure legend: " [a] Representative  images of indirect immunofluorescence of DIV6 hippocampal neurons cultured from P1 WT and Xrcc1Nes-Cre mouse pups, immunostained for ADP-ribose  (red), NeuN  to identify neurons (green), and counterstained with DAPI  (blue). Cells were pretreated with PARP inhibitor (10 µM) or vehicle for 2 h prior to fixation, with PARG  inhibitor (10 μM) additionally present for the final hour. Scale bar 10 µm. [b] Histogram of mean (± SEM) relative pan-ADP-ribose fluorescence in NeuN-positive hippocampal neurons pretreated with PARP inhibitor (5 µM) or vehicle for 5 h prior to fixation, with PARG inhibitor (10 μM) additionally present for the final hour. Neurons were cultured from WT (n = 6 mice, &gt; 180 cells per condition), Xrcc1Nes-Cre (n = 6, &gt; 180), Parp1+/-/Xrcc1Nes-Cre (n = 3, &gt; 90), and Parp1-/-/Xrcc1Nes-Cre (n = 3, &gt; 90). * indicates significant differences from WT (Kruskal-Wallis ANOVA, p = 0.0013 and Dunn's post-hoc tests). "
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: "Summary histogram of the mean (± SEM) of the integrated fluorescence responses for all individual synapses for each condition. Synapse number is the same as in (D-F). Responses are significantly higher in Xrcc1Nes-Cre synapses  versus both WT and Parp1-/-/Xrcc1Nes-Cre (one-way ANOVA, *p &lt; 0.0033, and pairwise student's t-tests)."
NCBI gene IDs:
"Cre: 2777477
Nes: 18008
Parp1: 11545
Xrcc1: 22594"
Figure legend: "C-D: Confocal  images (C) and quantification (D) of immunofluorescence staining  of STAT5b,  actin filaments (phalloidin) and nuclei  (DAPI) in cardiomyocytes  of cryosections from the hearts  of mice  transduced with either control- or ERBB4ECD-AAV. Panel D depicts quantification of colocalization  of STAT5b- and DAPI-specific signals. One dot in the boxplot corresponds to the median value in 20-50 images of stained  sections from one heart  (control AAV: n = 4, ERBB4ECD-AAV: n=5, biological replicates). Two-tailed unpaired T-test was used for statistics. Scale bar 10µm. White arrows point to cardiomyocyte nuclei. Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"ERBB4: 13869"
Figure legend: "E: Real-time RT-PCR  analysis of expression of the indicated STAT5b  target genes in the heart  of mice  transduced with either control- or ERBB4ECD-AAV. One dot corresponds to analysis of one heart  (n = 10; biological replicates combined from two replicate experiments). Two-tailed unpaired T-test was used for statistics. Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"ERBB4: 13869
STAT5b: 20851"
Figure legend: "F-G: Confocal  images (F) and quantification (G) of immunofluorescence staining  of myosin heavy chain in primary mouse cardiomyocytes. The cells were treated with either control or Stat5b-targeting shRNAs and stimulated with NRG-1 for two days. Myosin  immunoreactivity was used to quantify  cell area. One dot in the boxplot corresponds to one cell (Control no NRG-1: n = 83, control with NRG1: n=73, Stat5b#1 with NRG-1: n=80, Stat5b#2 with NRG1: n=75; technical replicates combined from four biological replicate experiments). Non-parametric Kruskal-Wallis ANOVA was used. Post hoc analyses were conducted with the Mann Whitney U-test and the resulting P-values corrected with the method of Benjamini, Krieger and Yekutieli. Scale bar 20µm. Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"Stat5b: 20851"
Figure legend: "H-I: Confocal  images (H) and quantification (I) of immunofluorescence staining  of STAT5b  and nuclei  (DAPI) in mouse cardiomyocytes. The cells were treated with either control or Erbb4-targeting shRNAs. Panel I depicts quantification of colocalization  of STAT5b- and DAPI-specific signals (left) and the cell perimeter  (right). One dot in the boxplots corresponds to one cell (Control: n = 24, Erbb4#1: n=26, Erbb4#2: n=35; technical replicates combined from two biological replicate experiments). Non-parametric Kruskal-Wallis ANOVA and Dunn's multicomparison test (colocalization  analysis) and Brown-Forsythe one-way ANOVA and Dunnett's multicomparison test (cardiomyocyte  size analysis) with multiple test correction were used for statistics. Scale bar 20µm. Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"Erbb4: 13869"
Figure legend: "J:  Real-time RT-PCR   analysis of expression of the indicated STAT5b   target genes in mouse cardiomyocytes. The cells were treated with either control, Stat5b- or Erbb4-targeting shRNAs and stimulated with NRG-1 for 30 minutes. One dot corresponds to the mean of technical repeats in one experiment and the whiskers the standard deviation (for each group n = 3; biological replicate experiments). One-way ANOVA and the Dunnett's multicomparison test with multiple test correction was used for statistics. *, P ≤ 0.05; ** P &lt; 0.01; ***, P &lt; 0.001 against control shRNA (Igf-1  panel: Stat5b#1 P = 0.0038, Stat5b#2 P = 0.0153,  Erbb4#1 P = 0.0188, Erbb4#2 P = 0.0288; Myc  panel: Stat5b#1 P = 0.0093, Stat5b#2 P = 0.0119, Erbb4#1 P = 0.0197,  Erbb4#2 P = 0.0512; Cdkn1a  panel: Stat5b#1 P = 0.0091, Stat5b#2 P = 0.0107,  Erbb4#1 P = 0.0235,  Erbb4#2 P = 0.048). Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"Cdkn1a: 12575
Erbb4: 13869
Igf-1: 16000
Myc: 17869
STAT5b: 20851
Stat5b: 20851"
Figure legend: "G: Real-time RT-PCR  analysis of expression of the indicated STAT5b  target genes in primary mouse cardiomyocytes  treated for 1-3 hours with the DMSO  buffer, the ERBB  kinase inhibitor AG1478, or dynasore, and stimulated or not for 30 minutes with  NRG-1. One dot corresponds to the mean of technical repeats in one experiment and the whiskers the standard deviation (for each group in  Igf-1 and Cdkn1a  panel: n = 6, for each group expect the dynasore  group in Myc  panel: n=4, for the dynasore  group in Myc  panel: n=3 ; biological replicate experiments). One-way ANOVA and the Dunnett's multicomparison test with multiple test correction was used for statistics. *, P &lt; 0.05; ** P &lt; 0.01; ***, P &lt; 0.001; against DMSO  + NRG-1 treatment (Igf-1 panel: DMSO  without NRG-1 P = 0.0041, AG1478 P = 0.0082, dynasore P = 6.61e-06; Myc  panel: DMSO  without NRG-1 P = 0.0386, AG1478 P = 0.0037, dynasore P = 0.0064; Cdkn1a  panel: DMSO  without NRG-1 P = 0.0123, AG1478 P = 0.0064, dynasore P = 0.0092). Data information: For all boxplots the central band represents the median, the box the interquartile range and whiskers the whole range of values."
NCBI gene IDs:
"Cdkn1a: 12575
Igf-1: 16000
Myc: 17869
STAT5b: 20851"