EMBO/SourceData huggingface dataset:
Figure legend: "(E) Fluorescence imaging in the CHT  of cmyb:GFP embryos  injected with control- and slco2b1-MOs  and treated with PGE2. (F) Quantification of GFP  positive cells. Statistical analysis: one-way ANOVA, multiple comparison, *P &lt; .01; ****P &lt; .0001. Centre values denote the mean, and error bars denote s.e.m. Data information: Scale bar 200μm (E)."
NCBI gene IDs:
"slco2b1: 792084"
Figure legend: "(A) Fluorescence imaging of the CHT  of double transgenic cd45:CFP-NTR/runx1:mcherry embryos  injected with control- or slco2b1-morpholinos, after treatment with DMSO. (B) Fluorescence imaging of the CHT  of double transgenic cd45:CFP-NTR/runx1:mcherry embryos  injected with control- or slco2b1-morpholinos, after treatment with DMSO+MTZ. (C) Fluorescence imaging of the CHT  of double transgenic cd45:CFP-NTR/runx1:mcherry embryos  injected with control- or slco2b1-morpholinos, after treatment with DMSO+MTZ+PGH2. (D) Quantification of runx1:mcherry  positive-cells. Each dot represents the number of red cells for each embryo  (biological replicates). Each experiment has been repeated three independent times. (E) Quantification of cd45:CFP  positive-cells. Each dot represents the number of blue cells for each embryo  (biological replicates). Each experiment has been repeated three independent times.Data information for (D) and (E): Statistical analysis was completed using one-way ANOVA, multiple comparison test. ****P&lt;.0001. Centre values denote the mean, and error bars denote s.e.m Scale bar 200μm (A-C)."
NCBI gene IDs:
"slco2b1: 792084"
Figure legend: "(A) WISH  for cmyb  at 60hpf in  kdrl:Gal4+ embryos  injected with control- and slco2b1- morpholinos  and/or co-injected with the Tol2-UAS:slco2b1 vector and tol2  mRNA. The region indicated by the dashed bas been magnified and shows in (B). (C) Quantification and statistical analysis were completed using one-way ANOVA, multiple comparison test. ****P&lt; .0001. Centre values denote the mean, and error bars denote s.e.m. Each dot represents the number of cmyb-expressing cells for each embryo  (biological replicates). Each experiment has been repeat three independent times."
NCBI gene IDs:
"tol2: 
Tol2: 
cmyb: 30519
slco2b1: 792084"
Figure legend: "Automated microinjection was performed on organotypic slices of mouse E14.5 dorsal telencephalon using Dx-A488 along with mRNA for RFP Organotypic slices of mouse E14.5 dorsal telencephalon injected with Dextran-A488 (green) and RFP poly-A+-mRNA. After 24h in culture the progeny expresses RFP (magenta; blue: DAPI, n = 17 cells injected, 8 RFP positive). Percentage of RFP-positive and RFP-negative cells (n = 17 cells injected)."
NCBI gene IDs:
"RFP: "
Figure legend: "(F) qRT-PCR validation of Irf7 and Irf9 downregulation in RM1 cells from bone metastases (RMI BD) compared to parental RM1 cells, lung metastases (RM1 lung) and naïve bone marrow (BM) (n = 3 mice per group). p values represented as ** &lt; 0.005 and *** &lt; 0.0005 (Student's t test)."
NCBI gene IDs:
"Irf7: 54123
Irf9: 16391"
Figure legend: "(G) qRT-PCR of Irf7 and Irf9 downregulation in parental RM1 cells, RM1 cells from bone metastases (RM1 BD) in WT and Ifnar1 deficient (-/-) mice, with naïve BM from WT and Ifnar1 (-/-) animals for reference. (n = 3 mice per group; n = 1 for -/- BM control). p values represented as * &lt; 0.05 and ** &lt; 0.005 (Student's t test)."
NCBI gene IDs:
"Ifnar1: 15975
Irf7: 54123
Irf9: 16391"
Figure legend: "(A) Stability of Irf7 and Irf9 mRNA suppression by qRT-PCR in ex vivo bone-derived cells (RM1 BD Irf-; n = 7) in culture compared to a bone-derived line that showed initial loss during early passage (RM1 BD REV (EP); n = 3) then reverted to parental expression levels at late passage (RM1 BD REV (LP); n = 4) compared to parental RM1 (n = 3)."
NCBI gene IDs:
"Irf7: 54123
Irf9: 16391"
Figure legend: "(C) qRT-PCR analysis of Irf7 and Irf9 expression in RM1 BD Irf- cells ± 48 h treatment with MS275 (1 μM) (n = 7-9)."
NCBI gene IDs:
"Irf7: 54123
Irf9: 16391"
Figure legend: "(e) qRT-PCR analysis of Irf7 and Irf9 expression in parental RM1 cells (n = 4) 48 h post-contact culture with naïve BM (n = 6)."
NCBI gene IDs:
"Irf7: 54123
Irf9: 16391"
Figure legend: "(F) qRT-PCR analysis of Irf9 expression in parental RM1 cells ± 48 h co-culture with naïve BM under contact (non-transwell; NT) and transwell (0.4 μm filters that prevent cell contact) conditions (n = 6-8 per condition)."
NCBI gene IDs:
"Irf9: 16391"
Figure legend: "(G) qRT-PCR analysis of Irf9 expression in parental RM1 cells ± 48 h contact co-culture with naïve BM ± MS275 (1 μM) (n = 3-6 per condition)."
NCBI gene IDs:
"Irf9: 16391"
Figure legend: "(C) ELISA of IFN-α production by RM1 parental, RM1 BD Irf- base vector (BV) and RM1 BD Irf7 over-expressing (OE) with 24 h poly I:C stimulation (n = 4)."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(D) Bone metastasis-free survival in WT C57BL/6 mice subsequent to IC inoculation of RM1 BD Irf- BV (n = 9) and RM1 BD Irf7 OE (n = 7) cells (**p = 0.0028 by log-rank (Mantel-Cox) test)."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(E) Bone metastasis-free survival in Ifnar1 -/- mice harboring RM1 BD Irf- BV and RM1 BD Irf7 OE tumors (n = 4 per group) and representative bioluminescent imaging of tumor burden in leg bones (also shown in Appendix Fig S4F)."
NCBI gene IDs:
"Ifnar1: 15975
Irf7: 54123"
Figure legend: "(F) Immunohistochemical (IHC) staining for cerulean (anti-eCFP; green) on naïve WT bone, RM1 parental, RM1 BD Irf- BV and RM1 BD Irf7 OE tumor-bearing bones derived from WT animals; and RM1 BD Irf7 OE tumor-bearing bones derived from Ifnar1 -/- animals at survival assay endpoints. Blue represents DAPI nuclear staining. Scale bar, 100 μm."
NCBI gene IDs:
"Ifnar1: 15975
Irf7: 54123"
Figure legend: "(G-I) FACS analysis of dormant (claret+) RM1 BD Irf- and RM1 BD Irf7 OE cells from bone at (G) day 11 post-IC injection (n = 4 per group) and (h) day 17 (n = 4-6 mice group/time point) with (I) D17 active (claret-) colony quantitation (mean +1) determined by multiphoton imaging and IMARIS interrogation (n = 9 bones from 3 mice per condition). Median shown. Upper and low box hinges denote first and third quartiles. Whiskers mark value limits."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(J) FACS analysis of IFN-γ+ and TNF-α+ CD8+ T cells (%) post-ICS induction of T cell (spleen-derived from tumor-bearing mice) activation by RM1 parental, RM1 BD Irf- BV and RM1 BD Irf7 OE cells (n = 6 per condition/group)."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(K) Mean quantitation of TRAP-stained osteoclasts differentiated with M-CSF ± RANKL in co-culture with RM1 BD Irf- BV and RM1 BD Irf7 OE cells with representative wells shown (n = 3 per condition M-CSF; n = 6 per condition M-CSF + RANKL; also shown in Appendix Fig S4J). Scale bar represents 100 μm."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(C) FACS analysis of IFN-γ+ CD8+ T cells (%) post-ICS induction of T cell (spleen-derived from RM1 BD Irf- tumor-bearing mice) activation upon re-stimulation with RM1 cells and MS275, poly I:C or combination treatment, with NAC (no antigen-presenting cells) and RM1 BD Irf7 OE controls (n = 3; Irf7 OE control (C), n = 1)"
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "FACS analysis of IFN-γ+ CD8+ T cells (%) post-ICS induction of T cell (spleen-derived from RM1 BD Irf- tumor-bearing mice) activation upon re-stimulation with RM1 cells and MS275, poly I:C or combination treatment, with NAC (no antigen-presenting cells) and RM1 BD Irf7 OE controls (n = 3; Irf7 OE control (C), n = 1) with (D) representative FACS plots shown."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "D) post-IC inoculation with associated specific CD8+ memory T cell response. This is represented by FACS analysis of IFN-γ+ CD8+ T cells (%) post-ICS induction of T cell (spleen-derived from tumor-bearing mice) activation upon re-stimulation with RM1 BD Irf- cells and MS275, poly I:C or combination treatments in vitro, with NAC and RM1 BD Irf7 OE controls (n = 3; Irf7 OE control (C), n = 1). Representative FACS plots shown on right."
NCBI gene IDs:
"Irf7: 54123"
Figure legend: "(C). The table summarises the number and the sex of the embryos of the indicated genotypes, recovered from timed matings of Rif1+/- x Rif1+/- mice, in a C57BL/6J genetic background. The day of gestation (E) is indicated."
NCBI gene IDs:
"Rif1: 51869"
Figure legend: "(C). Time course analysis of Xist RNA expression by RT-qPCR during EB differentiation of Rif1+/+ (Rif1+/+ +OHT) and Rif1-/- (Rif1F/F +OHT) cells at the indicated timepoints. Rif1+/+ (solid line) and Rif1-/- (dashed line), female (black) and male (grey). Data are presented as mean ± standard deviation from three (female lines) or two (male lines) independent experiments. Statistical significance was determined using 2-way ANOVA comparing female Rif1+/+ to female Rif1-/- cell lines (****p ≤ 0.0001). Xist RT-primers Xist ex7 F and R were used. Values are normalised to a geometric mean consisting of the expression of Gapdh, Ubiquitin and β-Actin."
NCBI gene IDs:
"β-Actin: 11461
Gapdh: 14433
Rif1: 51869
Xist: 213742"
Figure legend: "RIF1 association with the Xist promoter assessed by ChIP-qPCR in two independent Rif1+/+ (Rif1+/+ +OHT, black) and two Rif1-/- (Rif1F/F +OHT, grey) female cell lines, in ESCs (A) P1 and P2 indicate the two Xist promoters, 5' indicates a region 2 kb upstream of Xist TSS. Inter1 and 2 are two intergenic regions that serve as negative controls. Peak and cRAD represent two previously identified regions of RIF1 association (positive control). See Appendix Fig. S2C for primer positions within Xist. Mean ±standard deviation from 3 independent experiments (A) and 2 independent experiments (B). p calculated by Student's two-tailed, paired t test comparing RIF1 association in Rif1+/+ cells on Xist P2 and P1 versus 5'. *p ≤ 0.05, ****p ≤ 0.0001 and ns=not significant."
NCBI gene IDs:
"Rif1: 51869
Xist: 213742"
Figure legend: "(D). Association of RIF1 with Xist P2 in the Fa2L cells (black) and a wild type female mESC line (grey), also harbouring one castaneus and one 129 X chromosome. Allele-specific ChIP-qPCR primers were used, cast indicates association with the castaneus Xist P2 and 129 indicates association with the 129 Xist P2. Enrichments are presented relative to input DNA. Mean ±standard deviation from 3 independent experiments. p calculated by Student's two-tailed, paired t test comparing RIF1 association with the castaneus and with the 129 X chromosome Xist P2, ***p ≤ 0.001. Below, schematic of the Xist/Tsix alleles in the Fa2L undifferentiated cells. (E). Association of RIF1 with Xist P2 in the Fa2L cells (black) upon differentiation. The analysis was performed as in (D). Mean ±standard deviation from 3 independent experiments. p calculated by Student's two-tailed, paired t test comparing RIF1 association with the castaneus and with the 129 X chromosome Xist P2. *p ≤ 0.05. Below, schematic of the Xist/Tsix alleles in the Fa2L differentiated cells. ("
NCBI gene IDs:
"Xist: 
Tsix: 22097
Xist: 213742"
Figure legend: "(A). Time course analysis of Xist expression by RT-qPCR during EB differentiation of female mESCs following knock down of Luciferase (Control, black) and Kap1 (Kap1 KD, grey), at the indicated timepoints. Data are presented as mean ±standard deviation from three independent experiments. Statistical significance was determined using 2-way ANOVA. Xist primers Xist ex3 F and Xist ex4 R were used. Normalisation was performed using a geometric mean consisting of the expression of Rplp0, Ubiquitin and Sdha. (*p ≤ 0.05). (B). RT-qPCR analysis of Xist expression levels during differentiation of the Fa2L cells, following expression of shRNA against Luciferase (Control, black) and Kap1 (Kap1 KD, grey), at the indicated timepoints. Mean ±standard deviation from a minimum of three independent experiments is presented. 2-way ANOVA was used to determine statistical significance. ns=not significant. ("
NCBI gene IDs:
"Luciferase: 
Kap1: 16483
Rplp0: 11837
Sdha: 66945
Xist: 213742"
Figure legend: "(C). Tsix RNA levels in female mESCs infected with shRNA directed against Luciferase (Control, black) and KAP1 (Kap1 KD, grey), during differentiation. Mean ±standard deviation from four independent experiments are shown. Statistical significance was determined using 2-way ANOVA. (**p ≤ 0.01). Values have first been normalised to a geometric mean consisting of the expression of Rplp0, Ubiquitin and Sdha."
NCBI gene IDs:
"Luciferase: 
KAP1: 16483
Kap1: 16483
Rplp0: 11837
Sdha: 66945
Tsix: 22097"
Figure legend: "(D). RNA FISH analysis of Tsix expression during differentiation of female mESCs expressing an shRNA directed against Luciferase (control) or against Kap1 (Kap1 KD). Left: Cells with no (0, red), one (1, grey) and two or more (2, black) Tsix foci were counted in two independent experiments, shown averaged. Statistical significance was determined by χ2. A minimum of 110 cells were counted per each time point for each line (**p ≤ 0.01, ****p ≤ 0.0001). Right: examples of cells with one (top) or two (central and bottom) Tsix FISH signals. Scale bars: 5μm."
NCBI gene IDs:
"Luciferase: 
Kap1: 16483
Tsix: 22097"
Figure legend: "(A). ChIP-qPCR analysis of KAP1 association with the indicated sites in wild type female mESCs (Rif1+/+, same as used in Fig. 3B but without OHT) and during early differentiation. ZFP629 is a well-characterised KAP1 associated region (positive control). Xite A and C indicate two regions within the Tsix enhancer Xite, Tsix region 1 indicates Tsix major promoter, Tsix region 2 indicates the Dxpas34 region, Tsix region 3 indicates a region slightly downstream of the Dxpas34 region. P1 and P2 indicate the two Xist promoters, 5' indicates a region 2 kb upstream of Xist TSS. Inter1 is an intergenic region. See Appendix Fig. S2C for the positions of the primers within Xist and Tsix. The data are presented as mean ±standard deviation from three (2d EB and 1d EB) and two (ESCs) independent experiments. Statistical significance was calculated by Student's two-tailed unpaired t test comparing RIF1 association to Xist P2 and P1 in 2d EB versus 1d EB (*p ≤ 0.05 and ns=not significant). In the inset, Tsix RNA levels were quantified by RT-qPCR during the differentiation of wild type female ESCs shown in Fig. 4F. The average of two experiments is shown. Tsix values are normalised to a geometric mean consisting of the expression of Rplp0, Ubiquitin and Sdha. Error bars indicate standard deviations."
NCBI gene IDs:
"Rif1: 51869
RIF1: 51869
Rplp0: 11837
Xite: 619209
Sdha: 66945
Tsix: 22097
Xist: 213742
ZFP629: 320683"
Figure legend: "(C). KAP1 association with Xist promoter in two independent Rif1+/+ (Rif1+/+ +OHT, black) and two Rif1-/- (Rif1F/F +OHT, grey) female mESC cell lines. Ezr is an additional region known to be associated with KAP1 in mESCs. Enrichments are presented relative to input DNA. Mean ±standard deviation from a minimum of three independent experiments per cell line are displayed. Statistical significance was determined using Student's two-tailed, unpaired t test comparing the KAP1 association with Xist P2 and P1 in Rif1+/+ versus Rif1-/- cells (**p ≤ 0.01)."
NCBI gene IDs:
"Ezr: 22350
Rif1: 51869
Xist: 213742"
Figure legend: "(C). HEXIM1 association with the Xist promoter (P1 and P2) in two independent Rif1+/+ and two Rif1-/- mESC lines, analysed by ChIP-qPCR. Tkbp1 and Myf5 are two control regions. As in the case of KAP1 association, deletion of Rif1 induces accumulation of HEXIM1 on Xist P1 and P2. Average ±standard deviation of two independent experiments. p values were calculated by two-tailed, unpaired, equal variance t-test. (**p ≤ 0.01, ***p ≤ 0.001)."
NCBI gene IDs:
"Tkbp1: 
KAP1: 16483
Myf5: 17877
Rif1: 51869
Xist: 213742"
Figure legend: "(E). Upon infection of Fa2L cells with shRNA against Kap1 or control, against Luciferase, HEXIM1 association with P2 was analysed, on both alleles, by ChIP-qPCR. As in the case of KAP1, HEXIM1 shows preferential association with Xist P2 on the Cast allele (future Xa, Control). The association is lost upon knock down of Kap1 (Kap1 KD). Myf5 serves as a negative and Neat1 as a positive control regions."
NCBI gene IDs:
"Luciferase: 
Kap1: 16483
KAP1: 16483
Myf5: 17877
Xist: 213742"
Figure legend: "(A) Quantification of AURKB transcripts in non-IPF lung derived resident-fibroblasts treated with indicated mitogens for 16hrs. *P &lt; 0.05, ***P &lt; 0.0005, and ****P &lt; 0.00005, 1-way ANOVA, (n=4)."
NCBI gene IDs:
"AURKB: 9212"
Figure legend: "(B) Quantification of AURKB gene transcripts in isolated fibroblasts from non-IPF and IPF lung stromal cell cultures. **P &lt; 0.005, unpaired t-test, (n=6)."
NCBI gene IDs:
"AURKB: 9212"
Figure legend: "(D) Immunoblots of Aurkb and Aurka in total lung lysates of normal (CCSP/-) and fibrotic (CCSP/TGFα) mice fed with Dox for 6wks. Quantification was performed using phosphor imager software and normalization was done using loading control GAPDH. *P &lt; 0.05, unpaired t-test, (n=4)."
NCBI gene IDs:
"CCSP: 22287
TGFα: 21802"
Figure legend: "(E) Immunostaining was performed using AURKB antibody in lung sections of control (CCSP/-) and TGFα (CCSP/TGFα) mice on Dox for 6wks (n=6). Representative images were obtained at 20X (low; Scale bar: 100µm) and 63X (high; Scale bar: 30µm) magnification. Dashed box indicates area of the section showing in high magnification."
NCBI gene IDs:
"CCSP: 22287
TGFα: 21802"
Figure legend: "(A) Human IPF lung fibroblasts were transiently transfected with control or WT1 siRNA for 72hrs and AURKB transcripts were quantified. ****P &lt; 0.00005, unpaired t-test, (n=4)."
NCBI gene IDs:
"AURKB: 9212
WT1: 7490"
Figure legend: "(B) Lung-resident fibroblasts from TGFα mice on Dox for 4 wks were transiently transfected with control or WT1 siRNA for 72hrs and AURKB transcripts were quantified. ****P &lt; 0.00005, unpaired t-test, (n=4)."
NCBI gene IDs:
"AURKB: 20877
TGFα: 21802
WT1: 22431"
Figure legend: "(C) Immunoblot analysis of AURKB and WT1 in the lysates of non-IPF fibroblasts transduced with control or WT1-adenoviral particles for 72hrs. **P &lt; 0.005, unpaired t-test, (n=3)."
NCBI gene IDs:
"WT1: 7490"
Figure legend: "(D) Quantification of AURKB transcripts in primary fibroblasts from IPF lung treated with either control or WT1 siRNA and stimulated with media or TGFα (100ng/mL) for 16hrs. ****P &lt; 0.00005, unpaired t-test, (n=4)."
NCBI gene IDs:
"AURKB: 9212
WT1: 7490"
Figure legend: "(E) Primary lung-resident fibroblasts were isolated from stromal cultures of TGFα mice placed on Dox for 8 wks. Cell lysates were prepared, and the ChIP assay was performed with anti-WT1 antibody or normal rabbit IgG as a negative control using AURKB gene promoter-specific PCR primers. Non-immunoprecipitated DNA is represented as input DNA (product size, 104 bp). ***P &lt; 0.0005, unpaired t-test, (n=2)."
NCBI gene IDs:
"AURKB: 20877
TGFα: 21802"
Figure legend: "(F) AURKB-promoter luciferase activity was measured in HEK293 cells transfected with control or WT1 overexpressing (OE) vector. ****P &lt; 0.00005, one-way-ANOVA, (n=6)."
NCBI gene IDs:
"AURKB: 9212
WT1: 7490"
Figure legend: "(C) Lung sections from control and TGFα mice fed with Dox food for 4 wks were stained for AURKB (green) and Ki-67(red). Merged image shows cells that co-express AURKB and Ki-67 (yellow)."
NCBI gene IDs:
"TGFα: 21802"
Figure legend: "(D) Quantification of Ki-67+ and AURKB+ double positive cells was performed using five confocal images per mice in control and TGFα mice. Images were obtained at 40X magnification. Scale bar: 50µm. ****P &lt; 0.00005, unpaired t-test, (n=4)."
NCBI gene IDs:
"TGFα: 21802"
Figure legend: "(E) Proliferation was measured in primary lung resident fibroblast isolated from either IPF lung or TGFα mice and treated with either control or AURKB siRNA. ****P &lt; 0.00005, unpaired t-test, (n=9-11)."
NCBI gene IDs:
"AURKB: 9212
TGFα: 21802"
Figure legend: "(F) Proliferation was measured in primary lung-resident fibroblasts isolated from stromal cultures of TGFα mice on Dox for 2 wks and transiently transfected with control or AURKB siRNA and stimulated with TGFα (20ng/mL) for 24hrs. ****P &lt; 0.00005, 1-way ANOVA, (n=9-11)."
NCBI gene IDs:
"AURKB: 20877
TGFα: 21802"
Figure legend: "(G) Quantification of CCNA2 and PLK1 transcripts in human IPF fibroblasts transiently transfected with control or AURKB siRNA for 72hrs. **P &lt; 0.005, ***P &lt; 0.0005, unpaired t-test, (n=3)."
NCBI gene IDs:
"AURKB: 9212
CCNA2: 890
PLK1: 5347"
Figure legend: "(H) Lung sections from control and TGFα mice fed with Dox food for 6 wks were stained for AURKB (green) and αSMA (red). Images were obtained at 40X magnification. Scale bar: 50µm. (n=4)."
NCBI gene IDs:
"TGFα: 21802"
Figure legend: "(I) Quantification of apoptotic cells using Incucyte ZOOM (Caspase-3/7- positive cells) in lung-resident fibroblasts isolated from IPF and TGFα mice on Dox for 6 wks and treated with control or AURKB siRNA for 72h. **P &lt; 0.005, ****P &lt; 0.00005, 2-way ANOVA, (n=4)."
NCBI gene IDs:
"AURKB: 9212
TGFα: 21802"
Figure legend: "(J) Quantification of BAK, BAX and FAS gene transcripts in human IPF lung resident fibroblasts treated with either control or AURKB siRNA for 72hrs. **P &lt; 0.005, ***P &lt; 0.0005, unpaired t-test, (n=3)."
NCBI gene IDs:
"AURKB: 9212
BAK: 578
BAX: 581
FAS: 355"
Figure legend: "(K) Quantification of Bak, Bax and Fas gene transcripts in lung resident fibroblasts isolated from TGFα mice and treated with either control or AURKB siRNA for 72hrs. *P &lt; 0.005, **P &lt; 0.005, unpaired t-test, (n=3)."
NCBI gene IDs:
"AURKB: 20877
Bak: 12018
Bax: 12028
Fas: 14102
TGFα: 21802"
Figure legend: "(B) Proliferation was assessed using BrdU incorporation assay in fibroblasts isolated from TGFα mice lung and treated with indicated doses of barasertib for total of 48hrs. ***P &lt; 0.0005, 1-way ANOVA, (n=9-11)."
NCBI gene IDs:
"TGFα: 21802"
Figure legend: "(C) Primary lung resident fibroblasts isolated from TGFα mice on Dox for 4 wks were treated with vehicle or 5µM barasertib for 48hrs and immunostained using PCNA antibody. Images were obtained at 40X magnification. Scale bar: 50µm. The number of PCNA-positive cells and total DAPI-positive cells were quantified using MetaMorph image analysis software. Proliferation is indicated as the percentage of proliferating cells in total DAPI-positive cells. ****P &lt; 0.00005, unpaired t-test, (n=3-4)."
NCBI gene IDs:
"TGFα: 21802"
Figure legend: "(E) Quantification of Col1α, Col5α and Fn1 gene transcripts in total lung of mice treated with vehicle or barasertib.***P&lt; 0.0005, ****P &lt; 0.00005, 1-way ANOVA, (n=8 mice/group)."
NCBI gene IDs:
"Col1α: 12843///12842
Col5α: 53867///12832///12831
Fn1: 14268"
Figure legend: "(C) Quantification of Aurkb, Plk1 and CcnA2 gene transcripts in total lungs of mice treated with vehicle or barasertib. **P&lt; 0.005, ***P&lt;0.0005, ****P &lt; 0.00005, 1-way ANOVA, (n=8 mice/group)."
NCBI gene IDs:
"Aurkb: 20877
CcnA2: 12428
Plk1: 18817"
Figure legend: "(D) Quantification of Col1α1, Col3α, Col5α, Col14, Col15, αSma, Ccna2, and Fas gene transcripts in total lungs of mice treated with vehicle or barasertib. *P&lt; 0.05, **P&lt;0.005 ***P&lt;0.0005, ****P &lt; 0.00005, 1-way ANOVA, (n=6 mice/group)."
NCBI gene IDs:
"αSma: 11475
Ccna2: 12428
Col14: 12818
Col15: 12819
Col1α1: 12842
Col3α: 12825
Col5α: 53867///12832///12831
Fas: 14102"
Figure legend: "(C) PTCH1 inhibited the Endo H-resistant glycosylation of SMO. HEK293T cells were co-transfected with the SMO and PTCH1 expression plasmids. After 48 h, the cells were harvested, lysed, and treated with indicated glycosidases. Results shown are representative of three independent experiments."
NCBI gene IDs:
"PTCH1: 19206
SMO: 6608"
Figure legend: "(E) Shh increased the Endo H-resistant form of SMO. Cells were transfected as indicated for 24 h, and incubated with ShhN conditioned medium for an additional 24 h. Cells were harvested and analyzed as in (C). Results shown are representative of three independent experiments."
NCBI gene IDs:
"Shh: 20423
ShhN: 20423"
Figure legend: "(G) Shh increased the Endo H-resistant form of endogenous SMO in MEF cells. Results shown are representative of three independent experiments."
NCBI gene IDs:
"Shh: 20423"