EMBO/SourceData huggingface dataset:
Figure legend: "(B) Western blots of F-actin to G-actin ratio with or without cLTP induction from Nedd4-2 WT or Nedd4-2 cKO cultured neurons. Schematic representation of the experimental design for cLTP inductions (top left). Representative western blots (bottom left) and quantification of F-actin to G-actin from Nedd4-2 WT or Nedd4-2 cKO (right) cultures after cLTP induction and recovery in normal aCSF for 10 or 30 min before lysis (n = 11 and 8 for Nedd4-2 WT and Nedd4-2 cKO cultures, respectively). For quantification, data from cLTP induction were normalized to data from control (CTL) condition."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(A) Western blots of Nedd4-2, total cofilin, phospho (p)-cofilin and GAPDH from Nedd4-2 WT or Nedd4-2 cKO forebrains at postnatal day 14. Representative western blots and quantification of total cofilin relative to GAPDH and p-cofilin relative to total cofilin (n = 6 for Nedd4-2 WT and Nedd4-2 cKO brains) were shown."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(B) Immunocytochemistry of cofilin, p-cofilin and dendritic marker MAP2 from Nedd4-2 WT or Nedd4-2 cKO cultured hippocampal neurons at DIV 16. Representative dendrites of Nedd4-2 WT and Nedd4-2 cKO neurons and quantification of total cofilin relative to MAP2 and p-cofilin relative to total cofilin are shown (n = 25 and 26 for Nedd4-2 WT and Nedd4-2 cKO neurons, respectively). Scale bar: 5 µm."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(C) Immunocytochemistry showing cofilin, p-cofilin, and dendritic marker MAP2 from Nedd4-2 WT or Nedd4-2 cKO cultured hippocampal neurons induced with or without cLTP at DIV 16. Representative dendrites and quantification from Nedd4-2 WT or Nedd4-2 cKO cultures are shown (n = 36-39 neurons). Scale bar: 5 µm. For quantification, data from cLTP induction were normalized to data from control (CTL) condition."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(A) Immunocytochemistry showing post-synaptic marker PSD-95 (red), pre-synaptic marker Synapsin-I (green), dendritic marker Map2 (blue) and co-localization of PSD-95 and Synapsin-I from Nedd4-2 WT or Nedd4-2 cKO cultured hippocampal neurons at DIV 16 treated with TAT, TAT-S3, or TAT-pS3 peptide for 12 hrs (10 μM). Representative secondary dendrites of Nedd4-2 WT (top left) and Nedd4-2 cKO (bottom left) neurons are shown. Quantification of colocalized synaptic puncta number and synaptic puncta area for Nedd4-2 WT (top right) and Nedd4-2 cKO (bottom right) neurons is on the right (n = 32-36 neurons). Scale bar: 5 µm."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(B) Patch-clamp recordings from Nedd4-2 WT or Nedd4-2 cKO hippocampal neurons treated with TAT, TAT-S3, or TAT-pS3 peptide for 12 hrs (10 μM) at DIV 14-17. Representative mEPSC traces from Nedd4-2 WT (top left) and Nedd4-2 cKO (bottom left) are shown. Quantification of mEPSC amplitude and frequency from Nedd4-2 WT (top right) and Nedd4-2 cKO (bottom right) neurons are shown (n = 22-24 neurons)."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "(C) Barnes maze test after intraperitoneally injecting TAT (n = 8) or TAT-pS3 (n = 8) peptide in Nedd4-2 WT mice (top), or TAT (n = 9) or TAT-pS3 (n = 8) peptides in Nedd4-2 cKO mice (bottom). Learning curve during 4 training days of Barnes maze task showing the escape latency, number of errors before escaping, and the quadrant occupancy on probe trial at day 5 and at day 12 were assessed."
NCBI gene IDs:
"Nedd4-2: 83814"
Figure legend: "B, Multiplexed activation of Ascl1, Lmx1a, NeuroD1 and of  Ascl1, Lmx1a,  Nr4a2  in  primary astrocytic   cultures. n=3, representative experiment from 2-3 independent experiments, additional data in supplement. Activation levels are depicted as fold change between cells transfected with and without sgRNAs. All levels were normalized to β-Actin. Error bars represent mean ± SD between technical replicates."
NCBI gene IDs:
"β-Actin: 11461
Ascl1: 17172
Lmx1a: 110648
NeuroD1: 18012
Nr4a2: 18227"
Figure legend: "A, Representative photomicrographs taken from the dorsal striatum 13 weeks after AAV-injection. In  mice injected with GFP  control virus, virtually all GFP positive cells depict an astrocytic morphology, many  GFP positive cells in ALNe-218 and ALN-treated animals show a neuron-like morphology. Data information: Scale bars indicate 20 µm."
NCBI gene IDs:
"GFP: "
Figure legend: "B, Immunohistochemical  analysis showing GFP+/GFAP+ double positive cells 13 wpi. Arrows indicate double positive GFP+/GFAP+ cells, arrowheads indicate GFP+/GFAP- cells. Quantification of GFAP+/GFP+ cells shows a significant decrease upon ALNe-218  and ALN-treatment (GFP  vs. ALNe-218 P=0.0064, GFP  vs. ALN  P=0.0002 and ALN  vs. ALNe-218  P=0.0127). Multiple comparison ANOVA F(2,7)=32.06. C,  Double immunostaining  for GFP  and the neuronal  marker NeuN. Arrowheads indicate double positive GFP+/NeuN+ cell 13 wpi. Quantification demonstrate a significant increase in NeuN+/GFP+ cells upon ALNe-218 and ALN-induction (GFP vs.  ALNe-218  P&lt;0.0001, GFP  vs. ALN  P&lt;0.0001 and ALN  vs. ALNe-218 P=0.0012). Multiple comparison ANOVA F(2,6)=170.3. Data information: Scale bars indicate 20 µm. Tukey's multiple comparisons test * P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001. n = 3-4 mice  per condition. Error bars represent mean ± SD."
NCBI gene IDs:
"GFP: "
Figure legend: "D, Double immunostaining  for GFP  and TH  as a marker for dopaminergic neurons in the  dorsal striatum of GFP-control,  ALNe-218 and ALN-treated animals. Arrowheads indicate GFP+/TH- cells with  neuronal morphology. No GFP+/TH+ cell could be detected in any of the experimental groups. Scale bars indicate 20 µm. Data information: Scale bars indicate 20 µm."
NCBI gene IDs:
"GFP: "
Figure legend: "B, RT-qPCR analysis of Ascl1 induction comparing the activation capacity of full-length versus split-dCas9 in Neuro2A cells (data in fold change normalized to non-activated control; dCas9 6116 ± 847.3, split-dCas9 4415 ± 748.8, n=3 biological replicates, box-and-whiskers plots indicate median, 25th-75th percentile and min/max whiskers)."
NCBI gene IDs:
"Ascl1: 17172
dCas9: 69900935"
Figure legend: "C, Immunocytochemistry  analysis of reprogrammed  primary astrocytes   cells 16 days after lentiviral  transduction revealed successful in vitro reprogramming into neurons  using CRISPRa. Astrocytes  are infected with two lentiviruses  expressing dCas9-VPR  in a intein-split version similar to the AAV-dCAS system but driven by a Tet-O promoter. Tet-O driven dsRed  and Ascl1 cDNA expressing construct were used as negative and positive controls respectively. Arrows indicate single MAP2 positive background neurons, arrowheads indicate double positive induced neurons. Scale bar indicates 20 µm. Data information: Abbreviations: OE - overexpression"
NCBI gene IDs:
"CRISPRa: 
dsRed: 
Tet-O: 
Ascl1: 17172
dCas9: 69900935"
Figure legend: "E, Multiplexed activation of  Ascl1/Lmx1a/NeuroD1, Ascl1/Lmx1a/Nr4a2 and Ascl1/ Lmx1a/ Nr4a2/Pitx3/FoxA2 in primary astrocytic  cells. n=2-3 biological replicates, one representative run is shown, additional data in supplement. Activation levels are depicted as fold change between cells transfected with and without sgRNAs. All levels were normalized to β-Actin. Error bars represent mean ± SD between technical replicates."
NCBI gene IDs:
"β-Actin: 11461
Ascl1: 17172
FoxA2: 15376
Lmx1a: 110648
NeuroD1: 18012
Nr4a2: 18227
Pitx3: 18742"
Figure legend: "A, Representative photomicrographs  taken from the dorsal striatum  13 weeks after AAV-injection. In the GFP  control condition, virtually all GFP  positive cells depict an astrocytic morphology, many GFP  positive cells in ALNe-218 and ALN-treated animals show a neuron-like morphology. Data information: Scale bars indicate 20 µm."
NCBI gene IDs:
"GFP: "
Figure legend: "B,  Immunohistochemical  analysis showing GFP+/GFAP+ double positive cells 13 wpi. Arrows indicate double positive GFP+/GFAP+ cells, arrowheads indicate GFP+/GFAP- cells. Quantification of GFAP+/GFP+ cells shows a significant decrease upon ALNe-218 and ALN-treatment (GFP  vs. ALNe-218 P=0.0083, GFP  vs. ALN  P&lt;0.0001 and ALN vs. ALNe-218 P=0.0006, multiple comparison ANOVA F(2,6)=79.76). C, Photomicrographs  showing GFP+/NeuN+ neurons  13 wpi. Arrowheads indicating GFP+/NeuN+ induced neurons. Quantification demonstrate a significant increase in NeuN+/GFP+ cells upon ALNe-218 and ALN-induction (GFP  vs. ALN P=0.0008, ALN vs.  ALNe-218  P=0.0092, multiple comparison ANOVA F(2,7)=21.74). Data information: Scale bars indicate 20 µm. Error bars represent mean ± SD. n = 3-4 mice  (b,c) per condition. Tukey's multiple comparisons test * P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001."
NCBI gene IDs:
"GFP: "
Figure legend: "A, Gait analysis using the CatWalk XT system reveals motor defects in 6-OHDA lesion model. Both dCAM and AAV-dCAS  animals transduce with AAV containing specific gRNAs show a significant improvement in different aspects of voluntary movement like the average speed of tread. Naive vs. 6-OHDA P=0.0063. AAV-dCAS: GFP  vs.  ALN P=0.015 multiple comparison ANOVA F(2,17)=12.81) Data information: Statistics: naive vs. 6-OHDA unpaired t-test (two-tailed) * P&lt;0.05, ** P&lt;0.01. GFP  vs ALN, GFP  vs  ALNe-218 and ALN vs ALNe-218 Tukey's multiple comparisons test * P&lt;0.05, ** P&lt;0.01. n = 4-8 mice  per condition."
NCBI gene IDs:
"GFP: "
Figure legend: "the stride length of  front paws: B, Naive vs. 6-OHDA P=0.0164. AAV-dCAS: GFP  vs. ALN  P=0.005, ALN  vs. ALNe-218  P=0.0042, multiple comparison ANOVA F(4,22)=9.9) Data information: Statistics: naive vs. 6-OHDA unpaired t-test (two-tailed) * P&lt;0.05, ** P&lt;0.01. GFP  vs ALN, GFP  vs  ALNe-218 and ALN vs ALNe-218 Tukey's multiple comparisons test * P&lt;0.05, ** P&lt;0.01. n = 4-8 mice  per condition."
NCBI gene IDs:
"GFP: "
Figure legend: "the duty cycle of left front paws. C, Naive vs. 6-OHDA P=0.0096. dCAM: GFP  vs. ALN P=0.036 and ALN vs. ALNe-218 P=0.0252, multiple comparison ANOVA F(2,20)=5.199). Data information: Statistics: naive vs. 6-OHDA  unpaired t-test (two-tailed) * P&lt;0.05, ** P&lt;0.01. GFP  vs ALN, GFP  vs ALNe-218 and  ALN  vs ALNe-218  Tukey's multiple comparisons test * P&lt;0.05, ** P&lt;0.01. n = 4-8 mice  per condition."
NCBI gene IDs:
"GFP: "
Figure legend: "D, In contrast to this, dopamine  dependent drug-induced behavior does not show rescue effects: amphetamine-induced  rotation analysis: change in rotational behavior in lesioned animals upon treatment with dopamine  releaser substance. Net rotation = ipsilateral rotation-contralateral rotation (naïve vs. 6-OHDA P=0.09). Data information: Statistics: naive vs. 6-OHDA unpaired t-test (two-tailed) * P&lt;0.05, ** P&lt;0.01. GFP  vs ALN, GFP  vs  ALNe-218 and ALN vs ALNe-218 Tukey's multiple comparisons test * P&lt;0.05, ** P&lt;0.01. n = 4-8 mice  per condition. CatWalk error bars represent mean ± SD. Rotation analysis error bars represent mean ± SEM."
NCBI gene IDs:
"GFP: "
Figure legend: "T cells were collected from age- and gender-matched WT, EGR1KO and EGR4KO mice. (A) CD4+ T cells were isolated from the spleen by negative selection before incubating with anti-CD3/CD28 antibodies for the indicated time periods. Levels of expression of EGR1, EGR2, EGR3 and EGR4 were measured by qPCR."
NCBI gene IDs:
"EGR1: 13653
EGR2: 13654
EGR3: 13655
EGR4: 13656"
Figure legend: "T cells were collected from age- and gender-matched WT, EGR1KO and EGR4KO mice. (B) WBC were collected from WT, EGR1KO and EGR4KO mice before staining and FACS analysis to determine composition (gating strategies in Appendix Fig S1). Subclasses of CD8+, CD4+ and Treg populations were determined as shown."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "WT and EGR4-/- CD4+ and CD8+ T cells were isolated from the spleen by cell sorting and stained with CFSE. A Cells were incubated under unstimulated conditions or stimulated with either anti-TCRβ or anti-CD3/CD28 antibodies for 0 to 5 days (mean±SEM; a minimum of 3 biological replicates were examined; each biological replicate includes 2 technical replicates)."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "WT and EGR4-/- CD4+ and CD8+ T cells were isolated from the spleen by cell sorting and stained with CFSE. B Dose dependence of EGR4-dependent differences in proliferation were also determined in both CD4+ and CD8+ cells aft er treatment with anti-TCRβ (E,F) or anti-CD3/CD28 (G,H) at the indicated concentrations before FACS analysis (Appendix Fig S2) (mean±SEM; a minimum of 3 biological replicates were examined; each biological replicate includes 2 technical replicates)."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "WT and EGR4-/- CD4+ and CD8+ T cells were isolated from the spleen by cell sorting and stained with CFSE. C,D Generation analysis was then assessed in WT, EGR1-/- and EGR4-/- CD4+ (C) or CD8+ (D) T cells after a 4 day incubation, under unstimulated conditions, or stimulated with either anti-TCRβ or anti-CD3/CD28 antibodies. Generation analysis was determined using FlowJo as depicted in Appendix figure S3 (mean±SEM; a minimum of 3 biological replicates were examined; each biological replicate includes 2 technical replicates).. EGR4 deletion significantly shifted the number of generations as determined by two-way ANOVA; loss of either EGR1 or EGR4 significantly changed the number of generations under resting or stimulated conditions (p &lt; 0.0001)."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "CD4+ and CD8+ T cells were isolated from the spleens of WT, EGR1KO and EGR4KO mice by cell sorting. (A-F) Cytokine secretion was measured by ELISA after incubating CD4+ (A,C,E) and CD8+ (B,D,F) T cells for 48 hrs in resting conditions (A,B) or stimulated with either anti-TCRβ (C,D) or anti-CD3/CD28 antibodies (E,F)."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "CD4+ and CD8+ T cells were isolated from the spleens of WT, EGR4KO mice by cell sorting. (G) Representative FACS plots depicting IFNγ levels after intracellular staining in WT and EGR4KO CD4+ and CD8+ T cells."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ and CD8+ T cells were isolated from the spleens of WT, and EGR4KO mice by cell sorting. (H) Comparison of IFNγ levels in WT vs. EGR4-/- T cells."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ T cells were isolated from the spleens of WT, EGR1KO and EGR4KO mice by cell sorting. (I) Representative FACS plots of CD4+ T cells incubated for 24 hrs under Th1 polarizing conditions before intracellular staining with IFNγ and IL-17 antibodies."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "CD4+ T cells were isolated from the spleens of WT, EGR1KO and EGR4KO mice by cell sorting. (J) Comparison of the number of Th1 polarized cells between WT, EGR1-/- and EGR4-/- CD4+ T cells."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "CD4+ T cells were isolated from the spleens of WT, EGR1KO and EGR4KO mice by cell sorting. (K) CD4+ T cells were incubated for 5 days under polarizing conditions before analysis of cytokine production by ELISA."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "(A) NFAT nuclear localization was measured was measured by immunocytochemistry in CD4+ and CD8+ T cells isolated by negative selection from WT, EGR1KO and EGR4KO mice. Cells were stimulated with anti-CD3/CD28 antibodies for the indicated time periods before fixation and staining. Nuclear localization of NFATc1 in CD4+ and CD8+ T cells was determined based on colocalization of NFATc1 and DAPI measured by Pearson analysis (mean±SEM; data are based upon 13 to 20 cells/data point from one experiment; experiment was repeated 3 times). EGR4-mediated differences in NFATc1 localization were determined by two-way ANOVA. NFAT localization was dependent upon EGR4 expression in both CD4+ (p=0.025) and CD8+ (p=0.039) T cells. Post-hoc analysis revealed EGR4-dependent differences at specific timepoints * p &lt; 0.05; *** p &lt; 0.001."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "(B) WT, EGR1KO and EGR4KO CD4+ T cells were isolated from the spleen by negative selection before plating on poly-lysine (control) or CD3/CD28 and loading with fura2. Ca2+ levels shown are from representative single cells measured from multiple experiments. (C) Scatter plots showing area under the curve (AUC) and variance for each cell measured under all conditions."
NCBI gene IDs:
"EGR1: 13653
EGR4: 13656"
Figure legend: "CD4+ and CD8+ T cells were isolated by negative selection from WT or EGR4-/-mice. (A) CD4+ and CD8+ T cells were loaded with Fura-2AM and plated on poly-D-lysine (2 hrs) before treating with biotinylated anti-CD3ε, followed by streptavidin where indicated. The average response of the majority of CD4+ (WT 74.2±3.2%; EGR4-/- 77.8±2.9%) and CD8+ (WT 79.4±3.5%; EGR4-/- 78.2±2.5%) cells that responded to streptavidin crosslinking of CD3 (mean±SEM; 40-60 cells/coverslip; 7 to 8 separate experiments)."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ and CD8+ T cells were isolated by negative selection from WT or EGR4-/-mice. (B) Cells were plated on anti-CD3/CD28 antibodies for 2 hrs; BTP2 (10 μM) was added where marked by the arrow."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ T cells were isolated by negative selection from WT or EGR4-/-mice. (C) CD4+ T cells were incubated with anti-CD3/CD28 antibodies for 6 hrs before measuring expression of IFNγ, IL-2, IL-17 and IL-4 by FACS analysis. Data are mean±SEM; a minimum of 3 biological replicates were examined; each biological replicate includes 2 technical replicates."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ T cells were isolated by negative selection from WT, EGR4-/- mice. (A) WT or EGR4-/- cells were loaded with Fura-2AM and plated on anti-CD3/CD28 antibodies for 2 hrs. Senicapoc (4 μM) or Tram-34 (5 μM) were added where marked by the arrow."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ T cells were isolated by negative selection from WT, EGR4-/- mice. (B) CD4+ T cells were isolated by negative selection from WT or EGR4-/- mice and incubated with anti-CD3/CD28 antibodies for 6 hrs in the presence or absence of either senicapoc, TRAM-34 or Shk-Dap22 before measuring expression of IFNγ levels by FACS analysis. Data are mean±SEM; 3 biological replicates were examined; each biological replicate includes 2 technical replicates. *** p &lt; 0.001; **** p &lt; 0.0001."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "CD4+ T cells were isolated by negative selection from WT, EGR4-/- mice. (C) To measure the effect of KCa3.1 inhibition of cell proliferation, WT and EGR4-/- CD4+ T cells were isolated from the spleen by cell sorting and stained with CFSE. Cells were incubated with anti-TCRβ antibodies in the presence of vehicle, Senicapoc (5 µM) or TRAM-34 (5 µM) for 4 days and then collected for FACS analysis. (D) Generation analysis was completed for all data using FlowJo software. Data are mean±SEM; 3 biological replicates were examined; each biological replicate includes 2 technical replicates."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "B16N melanoma cells stably expressing GFP-luciferase were injected into syngeneic WT and EGR4-/- mice by tail vein injection. (A) Luciferase expression was monitored by IVIS imaging at the indicated timepoints after luciferin injections. "
NCBI gene IDs:
"GFP: 
luciferase: 
EGR4: 13656"
Figure legend: "B16N melanoma cells stably expressing GFP-luciferase were injected into syngeneic WT and EGR4-/- mice by tail vein injection. (B-D) Mice were sacrificed on day 20 and tumors were counted. Data is presented as total/mouse (B), N=9 for each gender and genotype. Differences in luciferase activity measured by IVIS in Panel A were determined by two-way ANOVA. Changes in luciferase activity were significantly altered in EGR4 knockout mice (P &lt; 0.0112)."
NCBI gene IDs:
"GFP: 
luciferase: 
EGR4: 13656"
Figure legend: "B16N melanoma cells stably expressing GFP-luciferase were injected into syngeneic WT and EGR4-/- mice by tail vein injection. Mice were sacrificed on day 20 and tumors were counted. (B), lung tumors (C) and metastatic tumors (all tumors found outside of the lungs; D). N=9 for each gender and genotype. Differences in luciferase activity measured by IVIS in Panel A were determined by two-way ANOVA. Changes in luciferase activity were significantly altered in EGR4 knockout mice (P &lt; 0.0112)."
NCBI gene IDs:
"GFP: 
luciferase: 
EGR4: 13656"
Figure legend: "B16N melanoma cells stably expressing GFP-luciferase were injected into syngeneic WT and EGR4-/- mice by tail vein injection. All lung tumors were isolated from both WT and EGR4KO mice along with spleens and analysed for CD45+ cells. WT lung tumors were 5.407 ± 1.843% CD45+, while EGR4KO mice were 12.445 ± 2.239% CD45+. WT spleens were 83.92 ± 3.344% CD45+, while EGR4KO mice were 95.13 ± 1.856% CD45+. Relative distributions of T and myeloid cells within the CD45+ populations were determined by flow cytometric analysis. (E,F) Distributions of T and myeloid cells found within lung tumors (E) vs. the spleen (F)."
NCBI gene IDs:
"GFP: 
luciferase: 
EGR4: 13656"
Figure legend: "B16N melanoma cells stably expressing GFP-luciferase were injected into syngeneic WT and EGR4-/- mice by tail vein injection. All lung tumors were isolated from both WT and EGR4KO mice along with spleens and analysed for CD45+ cells. (G) Representative FACS plots depicting gating strategies for defining Treg cell populations in Panels E and F"
NCBI gene IDs:
"GFP: 
luciferase: 
EGR4: 13656"
Figure legend: "Tumors collected and analysed for exhaustion/anergy. Cells were collected and enriched for the immune cell population by Ficoll-Hypaque. WT cells were 36.9 ± 4.68% Thy1.2+, EGR4KO cells were 48.0 ± 0.58% Thy1.2+. (A) CD4+ and CD8+ T cells were stimulated in vitro with ionomycin/PMA before intracellular staining for IFNγ, IL-17 and IL-9 and FACS analysis."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "Tumors collected and analysed for exhaustion/anergy. Cells were collected and enriched for the immune cell population by Ficoll-Hypaque. WT cells were 36.9 ± 4.68% Thy1.2+, EGR4KO cells were 48.0 ± 0.58% Thy1.2+. (B,C) Representative FACS plots depicting gating strategies for defining cytokine expression in panels A to D"
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "Tumors collected and analysed for exhaustion/anergy. Cells were collected and enriched for the immune cell population by Ficoll-Hypaque. WT cells were 36.9 ± 4.68% Thy1.2+, EGR4KO cells were 48.0 ± 0.58% Thy1.2+. CD4+ and CD8+ T cells were stained with CD44, CD73, FR4 and PD1 to monitor the formation of anergic/exhausted T cells. (D) Percentages of each population."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "Tumors collected and analysed for exhaustion/anergy. Cells were collected and enriched for the immune cell population by Ficoll-Hypaque. WT cells were 36.9 ± 4.68% Thy1.2+, EGR4KO cells were 48.0 ± 0.58% Thy1.2+. CD4+ and CD8+ T cells were stained with CD44, CD73, FR4 and PD1 to monitor the formation of anergic/exhausted T cells. (E) Gating strategies."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "Tumors collected and analysed for exhaustion/anergy. Cells were collected and enriched for the immune cell population by Ficoll-Hypaque. WT cells were 36.9 ± 4.68% Thy1.2+, EGR4KO cells were 48.0 ± 0.58% Thy1.2+. (F) FR4 and PD1 levels in WT vs. EGR4-/- T cells."
NCBI gene IDs:
"EGR4: 13656"
Figure legend: "BMDMs  were treated with  palmitic acid  (0, 100 μM, or 200 μM) for 12 h and transfected with HT-DNA  (2 μg/mL) for 6 h before  RT-qPCR analysis of Ifna4  (K), IFNb1  (L) expression. Data information: Data are representative of at least two independent experiments. Mean ± SEM from triplicates of technical replicates, unpaired t test; ns, not significant; **, P˂0.005; ***, P˂0.001"
NCBI gene IDs:
"Ifna4: 15967
IFNb1: 15977"
Figure legend: "BMDMs  were treated with  palmitic acid (0, 100 μM, or 200 μM) for 12 h and transfected with HT-DNA (2 μg/mL) for 6 h before RT-qPCR analysis of , Cxcl10  (M) and Rantes  (N) expression. Data information: Data are representative of at least two independent experiments. Mean ± SEM from triplicates of technical replicates, unpaired t test; ns, not significant; **, P˂0.005; ***, P˂0.001"
NCBI gene IDs:
"Rantes: 20304
Cxcl10: 15945"