EMBO/SourceData huggingface dataset:
Figure legend: "A Domain structure of UBQLN2 in upper region. HEK 293 cells were transfected with FLAG-tagged UBQLN2-WT or ALS-linked mutants (∆UBA, ∆UBL, P497H or P509S), along with mCherry-Parkin. After 24 h, the cells were treated with 5 µg/mL A/O for 5 h. The cells were stained with anti-FLAG (green) and anti-TOM20 (gray) antibodies, and then were visualized using confocal microscopy. Scale bars, 10 µm. Insets were higher magnifications of the dashed box area. Scale bars, 2.5 µm."
NCBI gene IDs:
"FLAG: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "C The cells were transfected with mCherry-Parkin and HA-tagged UBQLN2-WT or ALS-mutants as A and subjected to immunofluorescent assay as in A, but were treated with 1 µg/mL A/O for 24 h. Scale bar, 10 µm. Yellow arrow indicates uncleared mitochondria."
NCBI gene IDs:
"HA: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "E HEK 293T cells were transfected with indicated siRNAs. After 24 h, the cells were re-transfected with FLAG-tagged UBQLN2-WT or mutants, along with mCherry-Parkin for 24h. Then the cells were treated with or without 1 µg/mL A/O for 18 h. The cell lysates were subjected to immunoblot with indicated antibodies."
NCBI gene IDs:
"FLAG: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "A HEK 293 cells were transfected with mCherry-tagged Parkin, HA-tagged UBQLN2, FLAG-tagged HSP70 and BFP-mito for 24 h, and were then treated with 5 µg/mL A/O for 4 h. The cells were stained with anti-HA (green) and FLAG (gray) antibodies, and were visualized using confocal microscopy. Scale bar, 10 µm. Right panel were higher magnifications of the dashed box area. Scale bars, 2.5 µm."
NCBI gene IDs:
"FLAG: 
HA: 
mCherry: 
HSP70: 3303
Parkin: 5071
UBQLN2: 29978"
Figure legend: "D HEK 293T cells expressing HA-tagged UBQLN2 and FLAG-tagged HSP70 (WT or ΔC) were incubated with 5 µg/mL A/O for 5 h. The cells were then subjected to immunoprecipitation assay using anti-FLAG antibody."
NCBI gene IDs:
"FLAG: 
HA: 
HSP70: 3303
UBQLN2: 29978"
Figure legend: "E HEK 293T cells expressing HA tagged UBQLN2-WT, ΔUBA or P497H) and FLAG-tagged HSP70 were incubated with 5 µg/mL A/O for 5 h. Immunoprecipitation assay were performed using UBQLN2 antibody."
NCBI gene IDs:
"FLAG: 
HA: 
HSP70: 3303
UBQLN2: 29978"
Figure legend: "G The quantification data of UBQLN2 recruitment to mitochondria after 5 µg/mL A/O treatment. HEK 293T cells were transfected with indicated siRNAs. The cells were re-transfected with HA-tagged UBQLN2-WT, mCherry-Parkin, along with BFP-mito, and treated with 5 µg/mL A/O for 5 h. The cells were subjected to immunofluorescent assay using anti-HA antibody. Data from three independent experiments were represented as means ± SD., Sixty cells were counted for each sample. **, p&lt;0.01, one-way ANOVA."
NCBI gene IDs:
"BFP: 
HA: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "A HEK 293T cells expressing mCherry-Parkin were pre-treated with proteasome inhibitor (20 µM MG132) for 12 h, and treated with 1 µg/mL A/O for 2 h. Then the cells were fractionated and proteins were immunoblotted for relative antibodies. B The level of Mfn2 (OMM protein), TOM20, COXIV and HSP60 was quantified and normalized relative to GAPDH, and was shown in A. The data from three independent experiments were presented as mean± SD., ns, not significantly different; **, p&lt;0.01; ***, p&lt;0.001, one-way ANOVA."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "HEK 293T cells were transfected with indicated siRNAs for 48 h. C The cells were re-transfected with mCherry-Parkin for 12 h and were pre-treated with or without 20 µM MG132 for 12 h and treated with1 µg/mL A/O for 2 h. The cell lysates were subjected to immunoblot with indicated antibodies. The data from three independent experiments were presented as mean± SD., ns, not significantly different; *, p&lt;0.05; **, p&lt;0.01, one-way ANOVA."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "HEK 293T cells were transfected with indicated siRNAs for 48 h. The cells were transfected with HA-tagged UBQLN2-WT or ∆379-462, along with mCherry-Parkin for 12 h. Then the cells were treated with 1 µg/mL A/O for 2 h. The cell lysates were subjected to immunoblot using indicated antibodies. G Relative densities from F were shown in G. The data from three independent experiments were presented as means ± SD., ns, not significantly different; *, p&lt;0.05; **, p&lt;0.01, one-way ANOVA."
NCBI gene IDs:
"HA: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "HEK 293T cells were transfected with indicated siRNAs for 48 h. H The cells were re-transfected with HA-tagged UBQLN2-WT, along with mCherry-Parkin for 12 h. Then the cells were pretreated with 20 µM MG132 for 12 h and treated with 5 µg/mL A/O for 4 h. Mitochondria isolated from cell lysates were treated with or without 10 µg/mL proteinase K (Pro.K) and then were subjected to immunoblot with indicated antibodies. Mito: the isolated mitochondria; Mito (Pro.K): mitochondria after Pro.K treatment. COXIV level in \"Mito (Pro.K)\" group was quantified and normalized relative to HSP60 in \"Mito group\". The data from three independent experiments were presented as mean± SD., ** p&lt;0.01, ***, p&lt;0.001, one-way ANOVA."
NCBI gene IDs:
"HA: 
mCherry: 
Parkin: 5071
UBQLN2: 29978"
Figure legend: "HEK 293T cells were transfected with indicated siRNAs for 48 h. I The cells were re-transfected with HA, HA-UBQLN2-WT or P497H, along with GFP-Parkin and FLAG-PHB2 for 20 h, and then were treated with or without 5 µg/mL A/O for 4 h. Images of situ PLA using rabbit anti-LC3B and mouse anti-FLAG antibodies. Blue: nuclei (DAPI); white dots: PLA positive puncta. Scale bar, 10 µm. J Over 500 cells were counted for each sample in I. The data from three independent experiments were presented as mean± SD., *, p&lt;0.05; ** p&lt;0.01, ***, p&lt;0.001, one-way ANOVA."
NCBI gene IDs:
"FLAG: 
GFP: 
HA: 
PHB2: 11331
Parkin: 5071
UBQLN2: 29978"
Figure legend: "A Mouse cortical neurons (DIV 7) were infected with lentivirus carrying mCherry-Parkin for 45 h, and then the neurons were treated with 5 µg/mL A/O for 5 h and subjected to immunofluorescent assay using anti-UBQLN2 (green) and anti-HSP60 (gray) antibodies. Scale bars, 10 µm. Lower panel were higher magnifications of the dashed box area. Scale bar, 2.5 µm."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "Mouse cortical neurons (DIV 7) were transfected with indicated siRNAs for 24 h. B The neurons were infected with lentivirus carrying mCherry-Parkin for 24 h and then were treated with 1 µg/mL A/O for 48 h. The neurons were stained with DAPI, anti-UBQLN2 (green) and anti-HSP60 (gray) antibodies and visualized. Scale bars, 10 µm. Yellow arrows indicate uncleared mitochondria."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "Mouse cortical neurons (DIV 7) were transfected with indicated siRNAs for 24 h. F The neurons were infected with lentivirus carrying mCherry-Parkin for 36 h and were treated with 100 ng/mL Antimycin A (Antimycin A alone, AA) and 1 µM DFP for 10 h. The neurons were subjected to immunofluorescent assay using anti-TOM20 (magenta) and anti-LAMP 2 (gray) antibodies. Scale bars, 10 µm. Right panel were higher magnifications of the dashed box area. Scale bars, 2.5 µm. Yellow arrows indicate the co-localization between mitochondria and lysosomes."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "Mouse cortical neurons (DIV 7) were transfected with indicated siRNAs for 24 h. H The neurons were infected with lentivirus carrying mCherry-Parkin for 24 h. Then, the neurons were treated with 100 ng/mL Antimycin A and 1 µM DFP for another 10 h and subjected to immunofluorescent assay using anti-MAP2 antibody. Scale bars, 10 µm. I, J The neurite length (I) and cell death (J) in more than 30 neurons were quantified from each group in H. The data from three independent experiments were presented as the means ± SD., *, p&lt;0.05; ***, p&lt;0.001, one-way ANOVA."
NCBI gene IDs:
"mCherry: 
Parkin: 5071"
Figure legend: "B HeLa cells stably expressing SEPT6-GFP were infected with S. flexneri AfaI for 4 h, harvested, and then tested with Co-IP. Empty vector and/or uninfected HeLa cells were used in parallel as control. GFP-trap magnetic agarose beads were used to isolate SEPT6-GFP from cells, and extracts were immunoblotted for SEPT6, GFP, SEPT7, or p62. The lysates were immunoblotted for GAPDH as control for cellular protein levels."
NCBI gene IDs:
"SEPT6: 23157"
Figure legend: "E HeLa cells were treated with control (CTRL), Drp1, or Mfn1 siRNA for 72 h. Whole cell lysates were immunoblotted for Drp1 or Mfn1 to show the efficiency of depletion. GAPDH was used as loading control. siRNA-treated cells were infected with S. flexneri for 4 h 40 min, then fixed for microscopy and stained for endogenous SEPT7 for quantitative confocal microscopy. Graph represents the mean % ± SEM of Shigella inside SEPT7 cages from at least 4 independent experiments per treatment. Student's t-test, ** = p &lt;0.01; *** = p&lt;0.001."
NCBI gene IDs:
"Drp1: 10059
Mfn1: 55669"
Figure legend: "A HeLa cells were treated with control (CTRL), SEPT7, or Drp1 siRNA for 72 h. Whole cell lysate of siRNA-treated cells were immunoblotted for GAPDH, SEPT7, or Drp1 to show the efficiency of siRNA depletion. GAPDH was used as loading control. siRNA-treated cells were labelled with MitoTracker Red CMXRos, and fixed for confocal microscopy. The scale bar represents 5 μm."
NCBI gene IDs:
"Drp1: 10059
SEPT7: 989"
Figure legend: "B HeLa cells were treated with control (CTRL), SEPT2, SEPT7, SEPT9, Drp1, or SEPT7+ Drp1 siRNA for 72 h, labelled with MitoTracker Red CMXRos, and fixed for quantitative confocal microscopy. Graph represents the distribution in length (μm; whiskers from min to max) of mitochondria in siRNA-treated cells from 3 independent experiments (analysis of at least 100 measurements per biological replicate). Student's t-test, *** = p&lt;0.001."
NCBI gene IDs:
"Drp1: 10059
SEPT2: 4735
SEPT7: 989
SEPT9: 10801"
Figure legend: "C HeLa cells stably expressing SEPT6-GFP were harvested, and then tested with Co-IP. HeLa cells expressing GFP were used in parallel as control. GFP-trap magnetic agarose beads were used to isolate SEPT6-GFP from cells, and extracts were immunoblotted for Drp1, SEPT2, SEPT7, or GFP. The lysates were immunoblotted for GAPDH as control for cellular protein levels."
NCBI gene IDs:
"SEPT6: 23157"
Figure legend: "D HeLa cells were treated with control (CTRL) or SEPT7 siRNA for 72 h, and whole cell lysates were immunoblotted for SEPT7, Drp1, phosphorylated Drp1 (P-Drp1), or Mfn1. GAPDH was used as loading control. Graph represents the mean % ± SEM of the relative amount of Drp1, P-Drp1, Mfn1, or SEPT7 proteins quantified by densitometry from 4 independent experiments. Student's t-test, ns = non-significant; ** = p&lt;0.01, *** = p&lt;0.001."
NCBI gene IDs:
"SEPT7: 989"
Figure legend: "E HeLa cells were treated with control (CTRL) or SEPT7 siRNA for 72 h, labelled with MitoTracker Red CMXRos, then fixed and labelled for endogenous P-Drp1 for quantitative confocal microscopy. Representative images shown here. The scale bar represents 1 μm. The recruitment of P-Drp1 to mitochondria was significantly decreased as compared to control cells as measured by Pearson's correlation coefficient from at least 5 independent experiments (mean ± SEM, analysis of at least 250 cells per biological replicate). Student's t-test, * = p&lt;0.05."
NCBI gene IDs:
"SEPT7: 989"
Figure legend: "F HeLa cells were treated with control (CTRL) or SEPT7 siRNA for 72 h, labelled with MitoTracker Red CMXRos, then fixed and labelled for endogenous Mfn1 for quantitative confocal microscopy. Representative images shown here. The scale bar represents 1 μm. The recruitment of Mfn1 to mitochondria was significantly increased as compared to control cells as measured by Pearson's correlation coefficient from at least 5 independent experiments (mean ± SEM, analysis of at least 250 cells per biological replicate). Student's t-test, * = p&lt;0.05."
NCBI gene IDs:
"SEPT7: 989"
Figure legend: "A HeLa cells stably expressing SEPT6-GFP were treated with Drp1 siRNA for 72 h, transfected with mito-BFP 24 h, and then infected with S. flexneri-mCherry for 2 h for live confocal microscopy. Representative image shows interplay between two Shigella-septin cages and fused mitochondria. The scale bar represents 1 μm. See also Movie EV3."
NCBI gene IDs:
"Drp1: 10059"
Figure legend: "B HeLa cells were treated with Drp1 siRNA for 72 h, and then infected with x-light Shigella mCherry for 4 h 40 min for quantitative confocal microscopy. IPTG was added 30 min prior to fixation, and then samples were labelled with antibody for SEPT7. Graph represents mean % ± SEM of Shigella responding to IPTG outside (-) or inside (+) SEPT7 cages from 3 independent experiments. Student's t-test, *** = p&lt;0.001."
NCBI gene IDs:
"Drp1: 10059"
Figure legend: "D HeLa cells were either kept uninfected as a control or infected with S. flexneri M90T or S. flexneri ΔIcsA for 4 h 40 min. Samples were labelled with MitoTracker Red CMXRos, and fixed for quantitative confocal microscopy. Boxplots show length of mitochondria (μm; whiskers from min to max) in uninfected cells (CTRL), surrounding S. flexneri M90T (+M90T) or S. flexneri ΔIcsA (+ΔIcsA) from 3 independent experiments (analysis of at least 250 measurements per biological replicate). Student's t-test; *** = p&lt;0.001."
NCBI gene IDs:
"IcsA: 876473"
Figure legend: "Quantitative RT-PCR shows that Lpin1 expression is depleted in the skeletal muscles Gastrocnemius (GC) and Tibialis Anterior (TA), but not in White Adipose Tissue (WAT) from 3-month-old HSACre/+/Lpin1fEx2-3/fEx2-3 mice (HSA-Cre) compared to control mice. Expression levels are corrected for expression of the control gene Pinin and presented in the graph as fold change relative to control mice."
NCBI gene IDs:
"Pinin: 
Cre: 2777477
Lpin1: 14245"
Figure legend: "PAP1 and PAP2 activities were respectively substantially decreased and increased in TA, of HSA-Cre mice as compared to control mice, respectively (n = 6; *P&lt;0.05)."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Measurements of muscle/body weight ratio of GC and TA of 3-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Hematoxylin and Eosin (HE) staining of representative transverse sections of GC muscles from 3-month-old HSA-Cre and control mice. The black arrows indicate fibers with centrally located nuclei and yellow arrows indicate mononuclear cell infiltration. Scale bars: 40 μm"
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Relative force measurements after normalization of absolute tetanic force to GC muscle weight from 3-month-old HSA-Cre and control male mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Phosphatidic Acid (PA) levels in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Phospholipidome in HSA-Cre and control GC muscles. Proportions of indicated phospholipid classes in total phospholipids are represented. PC; Phosphatidylcholine, PE; Phosphatidylethanolamine, PI; Phosphatidylinositol, PS; Phoshpatidylserine, PA; Phosphatidic Acid, PG; Phosphatidylglycerol."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Amounts of indicated phospholipid classes normalized by protein content in HSA-Cre and control GC muscles. (D) Total phospholipid (PL) amount normalized by protein content in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Distribution of the total carbon number in acyl chains of PC in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Distribution of the number of double bonds in acyl chains of PC in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Distribution of the total carbon number in acyl chains of PE in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Distribution of the number of double bonds in acyl chains of PE in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "HE, Oil Red O (ORO), Cytochrome c Oxidase (COX) and Succinate Deshydrogenase (SDH) staining of representative frozen sections of TA muscle from 3-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantification of triacylglycerol (TAG) levels in GC muscles from 3-month-old HSA-Cre and and control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Content of TAG assessed by MS in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Cholesterol, (E) Cholesteryl ester (CE), (F) DAG was assessed by MS in HSA-Cre and control GC muscles."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantitative RT-PCR detection of the level of expression of Fatty acid synthase (Fasn), ATP citrate lyase (Acly), Thyroid hormone-inducible hepatic protein 1 (Thrsp1), Angiopoietin-like protein 4/ fast-induced adipose factor (ANGPTL4/Fiaf), Insulin-induced gene 1 (Insig1) and Peroxisome proliferator-activated receptor gamma (PPARγ) in TA from 3-month-old HSA-Cre and control mice. Expression levels are corrected for expression of the control gene Pinin and presented in the graph as fold change relative to control mice."
NCBI gene IDs:
"Pinin: 
Acly: 104112
ATP citrate lyase: 104112
Angiopoietin-like protein 4: 57875
ANGPTL4: 57875
fast-induced adipose factor: 57875
Fiaf: 57875
Cre: 2777477
Fasn: 14104
Fatty acid synthase: 14104
Insig1: 231070
Insulin-induced gene 1: 231070
Peroxisome proliferator-activated receptor gamma: 19016
PPARγ: 19016
Thrsp1: 21835
Thyroid hormone-inducible hepatic protein 1: 21835"
Figure legend: "Immunoblot analysis of Fasn in TA muscle of 3-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantitative RT-PCR measurement of Carnitine palmitoyltransferase 1B (Cpt1b) and Medium-chain acyl-CoA dehydrogenase (Mcad) in TA from 3-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Mcad: 11364
Medium-chain acyl-CoA dehydrogenase: 11364
Cpt1b: 12895
Carnitine palmitoyltransferase 1B: 12895
Cre: 2777477"
Figure legend: "Immunoblot analysis of full length and N-terminal SREBP1c and SREBP2 protein levels in GC muscle of 4-month-old HSA-Cre and control mice (n=7-4). The ratio of the densitometric assay of the N-term and Full length forms is presented."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Metabolomics profile of GC muscle of 4-month-old HSA-Cre and control mice presented as heatmap visualization and hierarchical clustering analysis of compounds"
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Fold change of phospholipid precursors in GC muscles of 4-month-old HSA-Cre and control mice measured by liquid-chromatography (LC) tandem mass spectrometry (MS/MS) (n=7-4, *P&lt;0.05)."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Fold change of Lactate/Pyruvate, Pyruvate/AcetylCoA, Pyruvate/Citrate, ATP/AMP, and ATP/ADP ratios in GC muscles of 4 month-old HSA-Cre and control mice (n=7-4, *P&lt;0.05)."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "UPR activation was analysed by immunoblotting with indicated antibodies in GC muscles of 4-month-old HSA-Cre and control mice (n=7-4). Quantification by densitometric analyses of Fgf21, Bip, sXbp1 Atf6 cleaved form, p-Eif2a and Chop protein levels is presented as a graph. Data are normalized to Gapdh and expressed as a fold change relative to the control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantitative RT-PCR measurement of Fgf21, Gdf15, Bip/Grp78, hsp90b1, and Edem1 in GC muscles from 4-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477
Edem1: 192193
Fgf21: 56636
Gdf15: 23886
hsp90b1: 22027
Bip: 14828
Grp78: 14828"
Figure legend: "EM analysis of EDL fibers of HSA-Cre mice revealed the presence of areas in which the non-junctional SR is more vesiculated and fragmented compared to control (black arrows). Remodeling of the SR is also accompanied by modification in the positioning of mitochondria (white arrows). A representative Calcium Release Unit (CRU) is indicated (empty arrow). Scale bars: 0.50 μm."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "The relative mtDNA copy number was determined by the ratio of mitochondrial DNA-encoded Cytochrome B (CytB) and nuclear-encoded NADH dehydrogenase (ubiquinone) flavoprotein 1 (Ndufv1) genes in GC and TA from HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477
CytB: 17711
Cytochrome B: 17711
Ndufv1: 17995
NADH dehydrogenase (ubiquinone) flavoprotein 1: 17995"
Figure legend: "Mitochondria-encoded gene and Nuclear-encoded gene expression levels by quantitative RT-PCR in GC muscles from 4-month-old HSA-Cre and control mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Electron micrographs of HSA-Cre EDL muscles of 3-month-old mice showed that mitochondria are almost exclusively positioned at the two sides of Z lines in EDL fibers from control mice (black arrows). On the other hand, in Lipin1 deficient EDL fibers mitochondria are more often clustered and display electron-dense contact sites (white arrows). Scale bars: 0.20 μm."
NCBI gene IDs:
"Cre: 2777477
Lipin1: 14245"
Figure legend: "Ultrastructural analysis of HSA-Cre EDL muscles revealed the presence of numerous autophagic vacuoles of different morphologies (autophagosomes and lysosomes, black arrows; multilamellar bodies, white arrow) containing amorphous material and possibly degraded mitochondria (M). Scale bars: 0.50 μm"
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Representative immunoblots from protein extract of GC muscles of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice, with the indicated antibodies."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantitative RT-PCR measurement of Fgf21 and GDF15 expression in GC muscle of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice."
NCBI gene IDs:
"Cre: 2777477
Fgf21: 56636
GDF15: 23886"
Figure legend: "Histological analysis of TA muscles of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice by Oil Red O staining to detect the neutral lipids. Scale bars: 40 μm."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "TAG level measurement in GC muscles of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "In vivo force measurements performed on GC muscles of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Representative staining of IgG positive fibers on frozen sections of TA of 6-month-old control, HSA-Cre untreated and HSA-Cre TUDCA-treated mice. The sections were stained with a secondary fluorescent anti-mouse IgG antibody. Quantification of the number of necrotic fibers over the total number of fibers."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Representative immunoblots from protein extract of GC muscles of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice, with the indicated antibodies."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Quantitative RT-PCR measurement of Fgf21 and GDF15 expression in GC muscle of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice."
NCBI gene IDs:
"Cre: 2777477
Fgf21: 56636
GDF15: 23886"
Figure legend: "Histological analysis of TA muscles of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice by Oil Red O staining to detect the neutral lipids."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "TAG level measurement in GC muscles of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "In vivo force measurements performed on GC muscles of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Representative staining of IgG positive fibers on frozen sections of TA of 4-month-old control, HSA-Cre untreated and HSA-Cre Bezafibrate-treated mice. The sections were stained with a secondary fluorescent anti-mouse IgG antibody. Quantification of the number of necrotic fibers over the total number of fibers."
NCBI gene IDs:
"Cre: 2777477"