EMBO/SourceData huggingface dataset:
Figure legend: "(e) Colocalization of UVRAG-C-Vps in early endosomes. HeLa cells were co-transfected with EGFP-Vps16 and Flag-UVRAG, stained with antibodies to LAMP1, LAMP2, EEA1 and Rab5, followed by confocal microscopy. All images are representative of at least three independent experiments. Scale bars, 5 μm. The raw data for a and b are shown in Supplementary Information, Fig. S6"
NCBI gene IDs:
"UVRAG: 7405
Vps16: 64601"
Figure legend: "(a) The N-terminal C2 domain or the C-terminal region of UVRAG interacts individually with Vps16. 293T cells were co-transfected with HA-Vps16, together with vector, Flag-UVRAG or Flag-UVRAG mutants. WCLs were immunoprecipitated with anti-Flag followed by immunoblotting with an anti-HA antibody."
NCBI gene IDs:
"Vps16: 64601"
Figure legend: "(b) HeLa cells were transfected with EGFP-Vps16 together with Flag-UVRAG mutants as indicated and stained with anti-Flag (red), followed by confocal microscopy. Scale bars, 5 μm."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(c) Interactions of the UVRAG C-terminal truncated mutants with Vps16. At 48 h post-transfection with HA-Vps16 and Flag-UVRAG C-terminal mutants, 293T WCLs were immunoprecipitated with anti-Flag, followed by immunoblotting with anti-HA."
NCBI gene IDs:
"UVRAG: 7405
Vps16: 64601"
Figure legend: "(d) UVRAG C2 domain or C-terminal region deletion abolishes Vps16 binding. At 48 h post-transfection with HA-Vps16 together with Flag-UVRAG or its mutants, 293T WCLs were immunoprecipitated with anti-Flag followed by immunoblotting with an anti-HA antibody."
NCBI gene IDs:
"UVRAG: 7405
Vps16: 64601"
Figure legend: "(a) HeLa.Vec and HeLa.UVRAG cells transfected with HA-Vps16 and GFP-LC3 were treated with 2 μM rapamycin for 2 h, and processed for confocal microscopy (left panel; scale bars, 5 μm). The percentage of GFP-LC3 punctae positive for HA-Vps16 staining was quantified (right panel; data are mean ± s.e.m., n = 60, *P 0.01)."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405
Vps16: 64601"
Figure legend: "(b) Effects of Vps16 on UVRAG-mediated autophagosome formation. Light microscopic quantification of autophagosomes in HeLa.Vec and HeLa.UVRAG cells (upper left panel) or in HCT116.Vec and HCT116.UVRAG cells (bottom left panel) when transfected with GFP-LC3 together with control siRNA or Vps16 siRNA (data are mean ± s.e.m., n = 60 for HeLa cells; n = 450 for HCT116 cells, *P 0.01; **P 0.001). Immunoblotting of Vps16 and tubulin are shown in the right panel."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405
Vps16: 64601"
Figure legend: "(a) UVRAG enhances the colocalization efficiency of GFP-LC3 with LAMP1. HeLa.Vec and HeLa.UVRAG cells transfected with GFP-LC3 were either untreated or treated with rapamycin (2 μM) in the absence or presence of bafilomycin A1 (0.1 μM) and stained for LAMP1. Insets highlight the colocalization. The percentage of LAMP1-positive autophagosomes was calculated in each setting (data are mean ± s.e.m., n = 200; 5 independent experiments, **P 0.01; *P 0.05)."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405"
Figure legend: "(b) UVRAG enhances the acquisition of CD63 by autophagosomes. The GFP-LC3-transfected HeLa.Vec and HeLa.UVRAG cells were treated as described in a and stained for CD63 and the percentage of CD63-positive autophagosomes was calculated (data are mean ± s.e.m., n = 200, **P 0.01; *P 0.05)."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405"
Figure legend: "(c) UVRAG promotes autophagic degradation of long-lived proteins. HeLa.Vec and HeLa.UVRAG cells were incubated for 16 h with L-3H-Leu (1 μCi ml-1). The degradation of long-lived proteins was measured at the indicated time points in complete medium (NC), EBSS alone (Starv.), or EBSS + 0.1 μM bafilomycin A1 (Starv.+BalfA1). Results (data are mean ± s.e.m. of triplicates) are representative of 2 independent experiments. Scale bars, 5 μm."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(a) Colocalization of GFP-LC3 and LAMP1. HeLa cells expressing wild-type or mutant UVRAG were transfected with GFP-LC3 and incubated under normal conditions or treated with rapamycin (2 μM). Autophagosome fusion with LAMP+ structures was analysed by confocal microscopy (left panel; rapamycin-treated) and quantified (right upper panel; data are mean ± s.e.m., n = 100). Arrows and insets show the LAMP1+ autophagosomes. The number of GFP-LC3-positive dots per cell was counted using a fluorescence microscope (right bottom panel; data are mean ± s.e.m., n = 60, *P 0.05; **P 0.0001)."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405"
Figure legend: "(b) Vps16 or Vps18 knockdown inhibits UVRAG-mediated autophagosome maturation. HeLa.Vec (left) and HeLa.UVRAG (right) cells were transfected with GFP-LC3 together with control siRNA, Vps16 siRNA, or Vps18 siRNA, followed by confocal microscopy. Arrows in the top panel denote the autophagosomes with LAMP1 staining. Immunoblotting of Vps16, or Vps18 and tubulin are shown in the middle panel. The percentage of LAMP1+ autophagosomes was calculated (data are mean ± s.e.m., n = 50, *P 0.05). Scale bars, 5 μm. The raw data of the immunoblots are shown in Supplementary Information, Fig. S6."
NCBI gene IDs:
"LC3: 440738///81631///84557
UVRAG: 7405
Vps16: 64601
Vps18: 57617"
Figure legend: "(a) UVRAG expression promotes the recruitment of Rab7 GTPase to autophagosomes. HeLa.Vec and HeLa.UVRAG cells transfected with GFP-Rab7 and RFP-LC3 were maintained either under normal conditions (NC) or treated with rapamycin, followed by confocal microscopy. Insets highlight the Rab7 acquisition of autophagosomes. The percentage of Rab7+-autophagosomes was quantified (right panel; data are mean ± s.e.m., n = 60, ***P 0.001; **P 0.01). Scale bars, 5 μm."
NCBI gene IDs:
"LC3: 440738///81631///84557
Rab7: 7879///338382
UVRAG: 7405"
Figure legend: "(b) Rab7 GTPase activity is increased with UVRAG expression. At 48 h post-transfection with GFP-Rab7 together with vector, UVRAG or UVRAGΔN(1-147) vector, 293T WCLs were used for immunoprecipitation with anti-GFP, followed by the Rab7 GTPase activity assay. Left panel: Autoradiographs of the GTP hydrolysis products analysed by TLC. Right panel: Quantification of the percentage of GTP hydrolyzed by Rab7 (data are mean n = 2 independent experiments)."
NCBI gene IDs:
"Rab7: 7879///338382
UVRAG: 7405"
Figure legend: "(c) Increased Rab7-RILP interaction UVRAG is expressed. HCT116.vector, HCT116.UVRAG and HCT116.UVRAGΔN(1-147) cells were transfected with GFP-Rab7 together with the GST-tagged Rab7 binding domain of RILP (GST-RILPRBD, residues 243-318). WCLs were used for GST pulldown, followed by immunoblotting with an anti-GFP antibody. The raw data of the immunoblots are shown in Supplementary Information, Fig. S6."
NCBI gene IDs:
"Rab7: 7879///338382
RILP: 83547
UVRAG: 7405"
Figure legend: "(a) EGF-stimulated endocytosis and post-endocytic trafficking. Uptake of Alexa Fluor 488-EGF (green) by HCT116.Vec (left panel) and HCT116.UVRAG (right panel) cells was followed by confocal microscopy. At the indicated times, cells were fixed and stained with antibodies to LAMP1 (red), and Topro-3 (blue, to stain the nucleus). Magnified images of the insets (fourth row) highlight trafficking of EGF towards LAMP1-positive structures."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(b) EGFR degradation. HCT116.Vec, HCT116.UVRAG and HCT116.UVRAGΔN(1-147) cells were treated with EGF (200 ng ml−1) at 37 °C for the indicated times. WCLs were subjected to immunoblotting with an anti-EGFR antibody. EGFR at each time point was quantified and indicated as a percentage relative to that at time zero (right panel; data are mean ± s.e.m., n = 3)."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(c) DQ-green BSA dequenching assay. Representative confocal microscopy images of DQ-BSA proteolysis (left panel) and flow cytometry analysis (right panel) of RAW264.7 cells expressing an empty vector (RAW264.7.Vec; dotted black line), wild-type UVRAG (red line) or UVRAGΔN(1-147) mutant (green line) loaded with 10 μg ml−1 DQ green-BSA for the indicated times. Background (grey line) represents samples without the BSA load."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(d) Effect of UVRAG on endosome fusion. RAW264.7.Vec and RAW264.7.UVRAG cells were pulse-labelled with Oregon green 514-avidin and dextran-568 for 10 min and chased for 30 min. Cells were then labelled with biotin-BSA for 10 min, and processed for confocal microscopy (left panel). In the absence of biotin-BSA, Oregon green514-avidin showed very low fluorescence (first row of confocal image). The percentage of dextran-568-labelled endosomes with visible Oregon green staining (denoted by arrows in the left panel) was calculated from approximately 20 different cells in 2 independent experiments (mean, right panel). Scale bars, 5 μm. The raw data of the immunoblots in b are shown in the Supplementary Information, Fig. S6."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "D.  SDH repression during acute phase of ENZA stress increased transcription of different AR-regulated genes without altering AR mRNAs levels in LNCaP cells. Data information: ENZA: enzalutamide.Data shown as mean ± SD of three independent experiments. Statistical analysis was performed using two tailed unpaired Student's t-test *, p&lt;0.05 and **, p&lt;0.01 compared between groups."
NCBI gene IDs:
"AR: 367"
Figure legend: "E-F. Overexpression of SDHA and SDHB subunits decreased AR protein levels (E) and AR transactivation (F) in LNCaP cells. Data information: ENZA: enzalutamide.Data shown as mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey's test *, p&lt;0.05 and **, p&lt;0.01 compared between groups."
NCBI gene IDs:
"SDHA: 6389
SDHB: 6390"
Figure legend: "C.  SDH-repressed LNCaP cells were able to maintain higher proliferation rate particularly during ENZA stress with or without Hsp27 silencing in real time cell proliferation assay. Data information: ENZA: enzalutamide. Data shown as mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA following Tukey's test for panels C (end points) . *, p&lt;0.05, **, p&lt;0.01 and ***, p&lt;0.001 compared between groups"
NCBI gene IDs:
"Hsp27: 3315"
Figure legend: "F.. Treatment with 5 µM STO-609 for 24 h in LNCaP cells inhibited AR upregulation induced by SDHA/SDHB subunit silencing."
NCBI gene IDs:
"SDHA: 6389
SDHB: 6390"
Figure legend: "(L) Histogram confirming the decreased number of Tuj1+ neurons in the ventral, but not dorsal, retina of Nr2f1 mutants. data are represented as mean ± SEM; N=3-4 Student t-test (*P&lt;0.05, **P&lt;0.01, ***P&lt;0.001). Scale bars: 50µm."
NCBI gene IDs:
"Nr2f1: 13865"
Figure legend: "(A) GSCs contain the higher levels of stemness markers (Nestin, SOX2, Musashi-1, NANOG, and OCT4) compared with their DFCs. Total RNAs from GSCs and DFCs were subjected to real-time PCR. The average relative amounts of GSCs were normalized with those of DFCs. Shown are means +S.D. of the normalized mRNA levels of GSCs obtained from the three independent experiments."
NCBI gene IDs:
"Musashi-1: 4440
NANOG: 79923
Nestin: 10763
OCT4: 5460
SOX2: 6657"
Figure legend: "(A) Real-time PCR analysis of the proteasomal subunits PSMB5, PSMB6, and PSMB7 in XO8 GSCs and XO8 DFCs. Shown are means +S.D. values from the three independent experiments following normalization to those of GSCs. For statistical analysis, student t-test (two-sided, one type) were used. ** P value=PSMB5: 0.053, PSMB6: 0.034, PSMB7: 0.067. (B) Similar to (A) except that the cells were treated with 50 nM MG132 for 17 hrs. Shown are means +S.D. values from the three independent experiments following normalization to those of control cells treated with DMSO. For statistical analysis, student t-test (two-sided, one type) were used. ** P value=PSMB5: 0.022, PSMB6: 0.08, PSMB7: 0.603."
NCBI gene IDs:
"PSMB5: 5693
PSMB6: 5694
PSMB7: 5695"
Figure legend: "(E) XO8 cells were treated with 50 nM MG132 in the absence or presence of 5μM actinomycin D (ActD) for 6 h. The level of ATF3 mRNA was measured using semi-quantitative real-time PCR in comparison with β-actin."
NCBI gene IDs:
"β-actin: 
ATF3: 467"
Figure legend: "(A) HEK293 cells were transfected with ATF3 shRNA or vector (pLKO1), treated with 50 nM MG132, and then subjected to immunoblotting."
NCBI gene IDs:
"ATF3: 467"
Figure legend: "(B) Individualized XO8 GSCs were transduced with lentiviral shRNAs against ATF3 or control as described in Materials and Methods. After four days, individualized GSCs were treated with 50 nM MG132, and subjected to immunoblotting."
NCBI gene IDs:
"ATF3: 467"
Figure legend: "(C) XO8 GSCs were transfected with control or CHOP siRNA for 48 hrs and then treated with 50 nM MG132 for 24 hrs. Cell viability was determined using the Trypan blue exclusion assay. Error bars represent the mean ± SD from three independent experiments (*P &lt; 0.05, n=3). For statistical analysis, student t-test (two-sided, one type) were used."
NCBI gene IDs:
"CHOP: 1649"
Figure legend: "(E) Wild-type and CHOP-/- MEF cells were treated with MG132 for 24 hrs. Cell viability was determined using trypan blue exclusion assay. Error bars represent the mean ± SD from three separate experiments (*P &lt; 0.05, n=3). For statistical analysis, student t-test (two-sided, one type) were used."
NCBI gene IDs:
"CHOP: 13198"
Figure legend: "(G) The expression of NOXA was assessed with real-time PCR using total RNA extracted from XO8 GSCs treated with 50 nM MG132 alone or in combination with each inhibitor of ER stress-driven apoptosis (salubrinal; 15 µM, STK064652; 20 µM, STK047915; 20 µM). The mean +S.D. values from the three independent experiments are shown after normalization to that of control cells treated with DMSO. For statistical analysis, student t-test (two-sided, one type) was used. * P value: salubrinal, 0.0003; STK064650, 0.0036; STK047915, 0.0019."
NCBI gene IDs:
"NOXA: 5366"
Figure legend: "(B) Measurements of Tie2-pY992 staining intensity at intercellular junctions in aortic arch endothelial cells showing lower values in the inner curvature than in the upper curvature of VE-PTPfl/fl mice but equally high values in both regions of VE-PTPiECKO mice. Normalized to mean value for outer curvature of VE-PTPfl/fl mice scaled to 1.0. Mean ± SEM, n = 6 mice/group."
NCBI gene IDs:
"VE-PTP: 19263"
Figure legend: "(C) HUVEC transfected with control siRNA or VE-PTP siRNA and exposed to static or laminar shear force at 15 dyn/cm2 for 30 min, followed by immunoprecipitation of Tie2. Left. Blot showing immunoprecipitates (top) or cell lysates (bottom) immunoblotted for pan-phosphotyrosine (antibody 4G10), Tie2, or VE-PTP. Ratio of staining for phosphorylated Tie2 and total Tie2 (Tie2-pY to Tie2, n = 4 experiments). Right. Relative Tie2-pY levels in HUVEC after control siRNA or VE-PTP siRNA. Normalized to static control = 1."
NCBI gene IDs:
"VE-PTP: 5787"
Figure legend: "(D) Effect of shear stress on the phosphorylation levels of Tie2 Y992 at cellular junctions. HUVECs treated with either control siRNA (left) or Tie2 siRNA (right), were exposed to 15 dyn/cm2 shear stress for 5 min. The resulting cells were fixed and stained for Tie2-pY992 (red) and VE-cadherin (green)."
NCBI gene IDs:
"Tie2: 7010"
Figure legend: "(E) HUVECs cultured on biotinylated gelatin were treated either with control siRNA, VE-PTP siRNA, Tie2 siRNA or VE-cadherin siRNA, then exposed to 15 dyn/cm2 shear stress for 30 minutes followed by 3 min incubation with Streptavidin-Alexa647 (red), washing, fixation and staining for VE-cadherin (green). (F and G) Quantification of Streptavidin leakage area per image. Mean ± SEM, n = 19 pooled in ten independent experiments (F), and n = 6 pooled in three independent experiments (G). D"
NCBI gene IDs:
"VE-cadherin: 1003
VE-PTP: 5787
Tie2: 7010"
Figure legend: "(D-E) Fluorescence intensity distributions in D and mean intensities in E of extravasated anti-fibrinogen antibody in inner curvature of ApoE-/-/VE-PTPfl/fl mice and ApoE-/-/VE-PTPiECKO mice on high-fat diet for 7 wk. Mean ± SEM, n = 7 mice/group."
NCBI gene IDs:
"ApoE: 11816
VE-PTP: 19263"
Figure legend: "(C) The ~110KD fragment (asterisk) is related to AMOT. Wild-type (WT) and AMOT knockout (KO) HEK293A cells were treated with 10 μM proteasome inhibitor bortezomib (BTZ) for 6 h and subjected to Western blotting analysis using the indicated antibodies."
NCBI gene IDs:
"AMOT: 154796"
Figure legend: "(F) Deletion of DDI2 blocks AMOT cleavage. Wild-type (WT) and DDI2 KO cells were serum-starved overnight and then treated with 1 μM LPA for 2 h."
NCBI gene IDs:
"DDI2: 84301"
Figure legend: "(G) Both DDI1 and DDI2 mediate AMOT cleavage. Ectopically expressed FLAG-tagged DDI1 or DDI2 rescues AMOT cleavage in DDI2 KO HEK293A cells."
NCBI gene IDs:
"FLAG: 
DDI1: 414301
DDI2: 84301"
Figure legend: "K) The aspartic catalytic-site mutant DDI2 exhibits a dominant negative effect through inactivation of wild-type DDI2 in HEK293A cells. Wild-type HEK293A cells were transfected with the indicated plasmids and treated with 1 μM LPA (K) before harvest."
NCBI gene IDs:
"DDI2: 84301"
Figure legend: "(B and C) The TNKS1/2-RNF146 axis is crucial for AMOT cleavage. Wild-type (WT), TNKS1/2 double KO (dKO) and RNF146 KO HEK293A cells were treated with the proteasome inhibitor MG132 for 6 h before harvest. Cell lysates were subjected to Western blotting analysis using the indicated antibodies."
NCBI gene IDs:
"RNF146: 81847
TNKS1: 8658"
Figure legend: "(H and I) AMOT cleavage states in different cell lines with NF2-wild-type or NF2-deficiency."
NCBI gene IDs:
"NF2: 4771"
Figure legend: "(F RNF146 are indispensable for AMOT cleavage in HUVECs. siRNAs were transfected into HUVECs, the cells were treated with 10 μM BTZ for 6 h before harvest. Protein quantifications are shown below."
NCBI gene IDs:
"RNF146: 81847"
Figure legend: "(I and J) Quantification of the number of sprouts per spheroid and the cumulative sprout length of HUVECs. Vector (n=20), AMOT-WT (n=18), AMOT-ΔTBD (n=14), AMOT-Δcut (n=15), AMOTp80 (n=16), AMOT-NT (n=17) and AMOT-CT (n=21)."
NCBI gene IDs:
"AMOT: 154796"
Figure legend: "(A and B) Knockdown of AMOT induces vascular development defects in zebrafish embryos. Lateral views of ISV and DLAV formation in Tg(flk:GFP) control and amot MO-injected zebrafish embryos at 30 h post-fertilization (hpf) are shown. Histogram depicts the quantification of normal and angiogenesis-defective embryos at 30 hpf (B). n: number of embryos analyzed. Dorsolateral anastomose vessels (DLAV), intersegmental vessels (ISV), dorsal aorta (DA) and posterior cardinal vein (PCV) are shown. Asterisks: defective ISV; dotted lines: dorsal edge of the embryo. Scale bar: 50 μm."
NCBI gene IDs:
"GFP: 
AMOT: 563421
amot: 563421
flk: 796537"
Figure legend: "(E and F) Non-cleavable AMOT mutant fails to support angiogenesis. Embryos were injected with amot MO together with mRNA encoding wild-type (WT) or mutant (ΔTBD, NT, CT, and Δcut) AMOT. Scale bar: 50 μm."
NCBI gene IDs:
"amot: 563421
AMOT: 154796"
Figure legend: "(G and H) AMOT-CT but not full-length AMOT could rescue impaired angiogenesis in ddi2-deficient embryos. Embryos were injected with ddi2 MO together with mRNA encoding wild-type AMOT (AMOT-WT) or AMOT-CT. Scale bar: 50 μm. (I and J) Nelfinavir (NFV) and XAV939 repress ISV development. Embryos were treated with 5 μM NFV, 10 μM XAV939 at 6 hpf for 24 h. Number of ISV defects was counted at 30 hpf. Scale bar: 50 μm."
NCBI gene IDs:
"AMOT: 154796
ddi2: 386644"
Figure legend: "(D) Ddi2iEC-KO mice show delayed retinal angiogenesis. Immunofluorescent images of whole-mount retinas stained for IB4 and ERG of control, Ddi2iEC-KO and Ddi2iEC-KO; CTiEC-TG mice at P6. White dash rectangles indicate regions of interest (ROI) are shown below at higher magnification. Scale bar: 100 μm."
NCBI gene IDs:
"Ddi2: 68817"
Figure legend: "(F) High magnification confocal images of filopodia extension at the leading edge of the P6 retina. Tip cells are labeled with IB4. Scale bar: 10 μm. (G) Quantification of the number and average length of filopodia in control (n=8), Ddi2iEC-KO (n=9) and Ddi2iEC-KO; CTiEC-TG (n=8) P6 retinal sprouts. Each data point represents the average of three measurements from one retina."
NCBI gene IDs:
"Ddi2: 68817"
Figure legend: "(H and I) Representative images and comparisons of Ki67 positive endothelial cells in control (n=8), Ddi2iEC-KO (n=8) and Ddi2iEC-KO; CTiEC-TG (n=7) P6 retinas. Each data point represents the average of three measurements from one retina. Scale bar: 50 μm."
NCBI gene IDs:
"Ddi2: 68817"
Figure legend: "(D) Nf2iEC-KO retinas show endothelial cell accumulation in the sprouting front and sparse vascular plexus in the region close to optic stalk. CT overexpression can partially rescue the phenotype. Immunofluorescent images of whole-mount P6 retinas stained for IB4 and ERG are shown. White rectangles indicate regions of interest (ROI). Scale bar: 100 μm."
NCBI gene IDs:
"Nf2: 18016"
Figure legend: "(F and G) High magnification confocal images and quantification of filopodia extension at the leading edge of the P6 retinas. Tip cells are labeled with IB4. Scale bar: 10 μm. Control (n=10), Nf2iEC-KO (n=10), Nf2iEC-KO; CTiEC-TG (n=10) and Nf2iEC-KO; ΔcutiEC-TG (n=8)."
NCBI gene IDs:
"Nf2: 18016"
Figure legend: "(H and I) Representative images and comparisons of Ki67 positive endothelial cells in control (n=8), Nf2iEC-KO (n=8), Nf2iEC-KO; CTiEC-TG (n=8) and Nf2iEC-KO; ΔcutiEC-TG (n=7) P6 retinas. Each data point represents the average of three measurements from one retina. Scale bar: 50 μm."
NCBI gene IDs:
"Nf2: 18016"
Figure legend: "(D) Blocking AMOT cleavage inhibits revascularization in the OIR model. Upper, IB4 staining of the whole retina of P17 WT and Δcut KI mice after OIR. Lower, higher magnification images of sprouting vessels from veins. Scale bars are shown in the figures."
NCBI gene IDs:
"AMOT: 27494"
Figure legend: "Cell count of Ronin fl/del and Ronin del/del ESC. Data represent mean ± SD, n = three independent experiments."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Annexin V cell death assay of Ronin fl/del and Ronin del/del cultured for 6 days. Two-tailed unpaired Student's t test, data represent mean ± SD, n = three independent experiments."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Ronin fl/del and Ronin del/del ESC stained for Ronin (left panel) or Oct4 (right panel). The nuclei are counterstained with DAPI."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Haematoxylin/eosin staining of paraffin sections of Ronin fl/del and ecto-Ronin teratomas."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "In vitro differentiation of Ronin fl/del, ecto-Ronin (grown in the presence of Dox) and Ronin del/del ESC."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Volcano plot of gene expression in Ronin del/del day 4 vs. Ronin fl/del (left panel) and Ronin del/del day 8 vs. Ronin fl/del ESC (right panel) with annotated naïve and primed pluripotency markers."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Volcano plot of gene expression in Ronin del/del day 8 vs. Ronin del/del day 4 ESC."
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Analysis of nascent protein synthesis using OP-puro assay. Ronin fl/del cells treated with protein synthesis inhibitor (cycloheximide) are used as negative control. The nuclei are counterstained with DAPI.   Quantification of the OP-puro signal, normalised to DAPI. Ordinary one-way Anova data represent mean ± SD; n = three independent experiments for each genotype.   "
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "Transmission electron microscopy analysis of mitochondrial structure in Ronin fl/del and Ronin del/del ESC. Mitochondria analysed, n &gt; 150 for each condition."
NCBI gene IDs:
"Ronin: 59016"