EMBO/SourceData huggingface dataset:
Figure legend: "(F) Changes in secondary dendrite GluA2 expression  in neurons  (DIV18) upon cLTP/cLTD  induction. Tau-/- neurons  were transfected with tau  at DIV12 (= tau-/-.tau) or mock transfected (tau-/-). Cells were stained  for GluA2, dendritic  marker Map2, and NSF  at 2 h post-cLTP/cLTD  induction or treatment with control artificial cerebrospinal fluid  (aCSF). Dendritic GluA2  cluster analysis was based on GluA2-Map2  binary intensity map. Representative insets from n = 10-20 dendrites  per condition from 2 mice  per genotype. Scale bar, 3 μm. (G) Quantification of GluA2  mean intensity relative to Map2  area and normalised to aCSF controls. Secondary dendrite GluA2  increased in tau-expressing (tau+/+ and tau-/-.tau) neurons  upon cLTP  induction. (n = 10-19 dendrites  from 3 biological replicates). Data information: Values are means ± S.E.M (normalized to control). Adjusted p-values: ****p &lt; 0.0001, ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test. "
NCBI gene IDs:
"Tau: 17762
tau: 4137
tau: 17762"
Figure legend: "(H) Representative immunostaining for whole-cell NSF  and GluA2  in tau+/+, tau-/- and tau-transfected tau-/- (tau-/-.tau) neurons  at 2 h post-cLTP/cLTD  induction or treatment with control artificial cerebrospinal fluid  (aCSF). (n = 3-6 biological replicates per condition with 10-20 cells each). Scale bar, 10 μm. Arrows indicate areas of marked co-distribution. (I) Co-localisation analysis of NSF  and GluA2  upon chemical LTP/LTD  induction. Note significantly higher proportional co-distribution of NSF  and GluA2  in tau-expressing (tau+/+ and tau-/-.tau) cells upon cLTP  induction. (n = 3-6 cells from 3 biological replicates). Data information: Values are means ± S.E.M (normalized to control). Adjusted p-values: ****p &lt; 0.0001, ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test. "
NCBI gene IDs:
"tau: 17762
tau: 4137"
Figure legend: "(B) Examples of sagittal sections of tau-/- and AAV.syn-hTau-injected tau-/- (tau-/-.AAVtau) mice  showing localized AAV-mediated expression of eGFP  or tau  and position of infusion cannulas  ending in the hippocampus  (dashed line). GluA1, immunolabelling  for GluA1 AMPA receptor subunit. Scale bar, 50 μm."
NCBI gene IDs:
"tau: 17762
Tau: 4137
tau: 4137"
Figure legend: "(C) Cued fear conditioning memory in tau+/+, tau-/- and tau-/-.AAVtau mice  upon bilateral infusion of TAT-NSF   or TAT-Scr. Due to infusion of Tat-fusion peptides occurring under brief isoflurane anaesthesia, a general anaesthesia (GA) control is included. Note that infusion of  TAT-NSF lowers memory performance to comparable levels in tau+/+, tau-/- and tau-/-.AAVtau mice. (n = 2-5 biological replicates) Data information: Values are mean ± 95% confidence intervals. Adjusted p-values: ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test."
NCBI gene IDs:
"tau: 17762
tau: 4137"
Figure legend: "(D) Novel object preference  (fold preference over familiar object) in tau+/+, tau-/- and tau-/-.AAVtau mice  upon bilateral infusion of TAT-NSF  or TAT-Scr (n = 2-5 biological replicates). Due to infusion of Tat-fusion peptides occurring under brief isoflurane  anaesthesia, a general anaesthesia (GA) control is included (n = 2 for GA). Note that infusion of TAT-NSF  lowers object discrimination to comparable levels in tau+/+, tau-/- and tau-/-.AAVtau mice. Data information: Values are mean ± 95% confidence intervals. Adjusted p-values: ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test."
NCBI gene IDs:
"tau: 17762
tau: 4137"
Figure legend: "(E) ATPase assay of recombinant NSF  upon incubation with tau coimmunoprecipitates  (tau5) from cortical   lysates of indicated genotypes, including from tau-transgenic mice  (ALZ17, Tau58/2) (n = 5-8 biological replicates). IgG, control immunoprecipitate  with non-specific IgG from tau+/+ lysate. Data information: Values are mean ± 95% confidence intervals. Adjusted p-values: ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test."
NCBI gene IDs:
"tau: 4137
tau: 17762"
Figure legend: "C-E. Luciferase reporter assay in HeLa  cells using the synthetic miR-Control or miR-182-3p in combination with the wild type (C) or the mutant 3'UTR of TRF2  construct (D) or the wild type 3'UTR of TRF1  (E). Data information: data are shown as mean ± SD. Three independent experiments were performed (n=3). P values are determined by Mann-Whitney test."
NCBI gene IDs:
"miR-182-3p: 406958
TRF1: 7013
TRF2: 54386"
Figure legend: "F,G. Western blotting for TRF2  expression in Telomerase   Positive (HeLa, HCT116, MDA-MB-231, MDA-MB-436) and ALT  positive (U2-OS, Saos-2) cells transiently transfected with miR-Control or miR-182-3p. Upper panel shows the quantification of TRF2  expression. Bottom panel, representative images are shown, actin  was used as loading control. Data information: data are shown as mean ± SD. Three independent experiments were performed (n=3). P values are determined by Mann-Whitney test."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "H. U2-OS cells transiently transfected with the miR-Control, miR-182-3p or miR-182-3p inhibitor were assayed by quantitative immunofluorescence  for TRF2  three days post-transfection. Left panel, representative images. Scale bar: 10µm. Right panel, quantification of TRF2  fluorescence intensity. a.f.u. arbitrary fluorescence units. N=number of analysed nuclei. Red bar indicates mean value. Data information: Three independent experiments were performed (n=3). P values are determined by Student's t-test; for (H)"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "E. Quantification of TIFs in MDA-MB-231 cells over-expressing TRF2  or an empty vector (pBabe), transfected with indicated miRNAs. The mean number of TIFs per nucleus  was quantified. F. Representative images and enlargements relative to the experiment described in E. White arrowheads indicate  co-localizations events. Scale bar: 10µm. Data information: data are shown as mean ± SD. Three independent experiments were performed (n=3). P values are determined by unpaired two-tailed t test. At least 60 nuclei  were analysed for each experimental condition. All the experiments were performed three days post-transfection with the indicated miRNAs"
NCBI gene IDs:
"TRF2: 54386"
Figure legend: "G. Quantification of PIFs in  MDA-MB-231  cells over-expressing TRF2  or an empty vector (pBabe), transfected with indicated miRNAs. The γH2AX  positive cells with ≥1 PIFs per nucleus  were analysed. H. Representative images relative to the experiment described in G. White arrowheads indicate  co-localizations  events. Scale bar: 10µm. Data information: For G data are shown as mean ± SD. Three independent experiments were performed (n=3). P values are determined by unpaired two-tailed t test. At least 60 nuclei  were analysed for each experimental condition. All the experiments were performed three days post-transfection with the indicated miRNAs"
NCBI gene IDs:
"TRF2: 54386"
Figure legend: "A,B. MDA-MB-436  and  MDA-MB-231  cells underwent two rounds of transfection with miR-Control, miR-182-3p or miR-182-3p inhibitor. Starting from the day of the second transfection, cell confluence was monitored by Incucyte every 24h up to a maximum of 3 days. The percentage of  cell confluence  was analysed. Data information: For A,B data are shown as mean ± SEM. three independent experiments were performed (n=3). P values are determined by unpaired two-tailed t test."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "E. Two-dimensional scatter plots of Annexin V  analysis performed in MDA-MB-436  at the end of the second cycle of transfection with miR-Control, miR-182-3p or miR-182-3p inhibitor. Red boxes indicate early and late apoptotic cells. F. Quantification of Annexin V positive cells (%) of experiment described in E. G. Two-dimensional scatter plots of  Annexin V analysis performed in MDA-MB-231 as described in E. H. Quantification of  Annexin V  positive cells (%) of experiment described in G. Data information: For F,H data are shown as mean ± SD. P values are determined by unpaired two-tailed t test. For F, H two different biological replicates were performed."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "I,J. MDA-MB-436 cells over-expressing TRF2  or an empty vector (pBabe) were transiently transfected with indicated miRNAs and  cell count (I) or apoptosis  (J) analysis was performed 72h post-transfection. Data information: For ,I,J, data are shown as mean ± SD. For I, three independent experiments were performed (n=3). P values are determined by unpaired two-tailed t test. For J, two different biological replicates were performed."
NCBI gene IDs:
"TRF2: 54386"
Figure legend: "A. Western blotting  for TRF2  expression in BJ  cells transiently transfected with miR-Control or miR-182-3p. The graph represents the quantification of three independent experiments. Representative images are shown, actin  was used as loading control. Unspecific bands are indicated with (*)."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "E.  Cells number  of BJ  cells was analysed by automatic cell count at the end of the second round of transfection with miR-Control or miR-182-3p. Data information: a student t test was used to calculate statistical significance. P values are indicated."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "G. β-Galactosidase assay  in BJ  cells after two rounds of transfection with mimic miR-Control or miR-182-3p. Left panel: analysis of β-Galactosidase positive cells. Right panel: representative images. Data information: a student t test was used to calculate statistical significance. P values are indicated."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "A,B. MDA-MB-231 (A) and MDA-MB-436  (B) tumor xenografts  were treated with LNPs-empty, LNPs-miR-Control or by LNPs-miR-182-3p when the tumors became palpable. Mice  were treated 6 times  by intravenous  tail vein injections with 20µg of LNPs-miR-Control, LNPs-miR-182-3p or equivalent volume of LNPs-empty as indicated in the scheduling. The mean of tumor volumes  (n=5 per group) is shown. Data information: For A, B data are shown as mean ± SD. P values are determined by unpaired two-tailed t test"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "C,D. Tumors from mice  treated in A and B were processed to measure miR-182-3p expression by  TaqMan qPCR. Data information: For C, D the line in the middle of the box plot denote a median value, the limits of box represent the interquartile range (25th to 75th percentiles), while, the whiskers denote the minimum to maximum values. , P values are determined by unpaired two-tailed t test;"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "G, H. Luminescent MDA-MB-436 cells were injected into the brain  and monitored by IVIS imaging system. After one week from implant, treatment with LNPs-miR-Control and LNPs-miR-182-3p was performed as indicated in A and B. Representative images from in vivo (upper panel) or ex-vivo (bottom panel) brain tumors are shown in G. Boxplots (H) show the measurement of photons for each  brain tumor (n=5 per group) acquired at the indicated times. Data information: For H, the line in the middle of the box plot denote a median value, the limits of box represent the interquartile range (25th to 75th percentiles), while, the whiskers denote the minimum to maximum values. For H, P values are determined by unpaired two-tailed t test;"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "A,B. PDTCs  #1 and #2 underwent two rounds of transfection with miR-Control or miR-182-3p. Three days after the second transfection, miR-182-3p  and TRF2  expression were analysed by TaqMan qPCR   and western blotting, respectively. Actin  was used as loading control. Data information: data are shown as mean ± SD. P values are determined by unpaired two-tailed t test For the experiments showed in A, B two or three biological replicates were performed, respectively."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "E. NSG mice  implanted  with breast  PDTX (#2) were treated with LNPs-empty, LNPs-miR-Control or LNPs-miR-182-3p  as indicated in the scheduling. Caliper measurement of tumors were taken at the indicated days. The mean of  tumor volumes (n=5 per group) is shown. Data information: data are shown as mean ± SD. P values are determined by unpaired two-tailed t test;"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "F.  miR-182-3p expression  of tumors from mice  treated in (E) was assayed by TaqMan qPCR. Data information: data are shown as mean ± SD. P values are determined by unpaired two-tailed t test"
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "A. Representative images of intestine  sections from mice  previously treated with LNPs-Empty or LNPs-miR-182-3p. H&amp;E staining  (scale bar: 200μm) and IHC  analysis with TRF2  or γH2AX  antibodies are shown (scale bar: 50μm). B,C. Quantification of TRF2 expression  as immunoreactivity score (IRS) (B) and of γH2AX  positive cells (%) (C) on intestine  samples. D. Representative H&amp;E (scale bar: 200μm), TRF2  and γH2AX  images of skin  samples corresponding to LNPs-Empty or LNPs-miR-182-3p treated animals (scale bar: 50μm). E,F. Quantification of TRF2 expression as immunoreactivity score (IRS) (E) and of γH2AX  positive cells (%) (F) on skin  samples. G. Representative H&amp;E (scale bar: 200μm), TRF2  and γH2AX  images of  bone marrow samples corresponding to LNPs-Empty or LNPs-miR-182-3p treated animals (scale bar: 50μm). H,I. Quantification of TRF2 expression  as immunoreactivity score (IRS) (H) and of γH2AX  positive cells (%) (I) on  bone marrow samples. Data information: For B,C,E,F,H,I, data are shown as mean ± SD. A Mann-Whitney test t test was used to calculate statistical significance. Four mice  per group were analysed the points represent the number of field analysed for each condition."
NCBI gene IDs:
"miR-182-3p: 406958"
Figure legend: "Representative Ca2+ signals in sperm from a patient with deafness-infertility syndrome lacking functional CatSper channels (CATSPER2-/-) (red) and a healthy donor (black), evoked by progesterone, PGE1, NH4Cl, or ionomycin. NH4Cl increases the intracellular pH. Bar graph: Amplitudes (n = 4; mean ± SD) of Ca2+ signals in CATSPER2-/- sperm"
NCBI gene IDs:
"CatSper: 117155
CATSPER2: 117155"
Figure legend: "Representative monovalent CatSper currents in CATSPER2-/- sperm (blue, green, orange, purple, brown) and in sperm from a healthy donor (black) and corresponding current-voltage relationship (right). The membrane voltage was stepped from -100 mV to +100 mV in increments of 10 mV from a holding potential of -80 mV. Outward and inward current amplitudes (mean ± SD) at +100 mV and -100 mV, respectively, in CATSPER2-/- sperm (color code: panel B) and sperm from healthy donors (black)."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "Representative immunocytochemical staining of control sperm from healthy donors and CATSPER2-/- sperm form DIS patients using antibodies directed against CatSper 3 (D) or CatSper 4 (E); DNA was labelled with DAPI (blue). Scale bars represent 10 µm."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "3D-STORM images in xy-projection of CATSPER2-/- sperm (left) labelled with the anti-CatSper 3 (upper panel) and anti-CatSper 4 (lower panel) antibody. Axial projection of the boxed regions (right). Scale bars represent 5 µm in xy-projections and 200 nm in axial projections."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "Representative distribution of rotation frequencies of freely swimming CatSper-deficient sperm incubated under non-capacitating (0 mM bicarbonate; black; n = 73) and capacitating conditions (25 mM bicarbonate; red; n = 272)."
NCBI gene IDs:
"CatSper: 117155"
Figure legend: "Rolling frequency (mean ± SD) of freely swimming CATSPER2-/- sperm incubated under non-capacitating (0 mM bicarbonate, n = 1009) and capacitating (25 mM bicarbonate, n = 946) conditions."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "Rolling frequency (mean ± SD) of freely swimming CATSPER2-/- sperm in 0 (n = 457), 0.2 (n = 389), and 1 % (w/v) (n = 187) methyl cellulose."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "Image series of a CATSPER2-/- sperm cell optically trapped perpendicular to the optical axis; images were obtained at t = 0, 75, 150, and 225 ms. Scale bar represents 10 µm. Image series of a CATSPER2-/- sperm cell optically trapped parallel to the optical axis; images were obtained at t = 0, 60, 195, and 320 ms. The red bar indicates the 360° rotation of the tip of the head. Scale bar represents 10 µm. Representative time courses of the rotation frequencies of optically trapped CATSPER2-/- sperm (each sperm is represented in a different color). Error bars indicate the full width at half prominence of the frequency peaks determined by the fast Fourier analysis."
NCBI gene IDs:
"CATSPER2: 117155"
Figure legend: "Left: Bright-field image series of a freely swimming wild-type sperm cell at t = 0, 151, and 303 ms. Scale bar represents 10 µm. Right: Rotation frequency (mean ± SD) of 24 freely swimming wild-type sperm. Left: Bright-field image series of a freely swimming Catsper1-/- sperm cell at t = 0, 151, and 294 ms. Scale bar represents 10 µm. Right: Rotation frequency (mean ± SD) of 24 freely swimming Catsper1-/- sperm."
NCBI gene IDs:
"Catsper1: 117144"
Figure legend: "A, B Bottom panels: gene annotation track (mm10); the activated gene is marked in green and the location of the TALE-VP64 target sequence is shown by the vertical dashed green line. Middle panels: DamID tracks of NL interactions in control cells (&quot;control&quot;, blue line) and cells expressing TALE-VP64 (&quot;experimental&quot;, red line). n indicates the number of independent biological replicates that were combined. Noise was suppressed by a running mean filter of indicated window size. Shading between the lines corresponds to the color of the sample with the highest value. Arrowheads on the right-hand side mark the 5th and 95th percentiles of genome-wide DamID values. Top panels: domainograms; for every window of indicated size (vertical axis) and centered on a genomic position (horizontal axis) the pixel shade indicates the ranking of the change in DamID score (experimental minus control) in this window compared to the genome-wide changes in DamID scores across all possible windows of the same size. Blue: DamID score is highest in control samples; red: DamID score is highest in experimental samples (color key on the right of panel (a)). In (a) activation of Sox6 was the experimental perturbation, activation of Nrp1 (which is located on a different chromosome) served as control; in (b) activation of Nrp1 was the experimental condition and activation of Sox6 served as control."
NCBI gene IDs:
"Nrp1: 18186
Sox6: 20679"
Figure legend: "(a-b) Plots as in Figure 1, showing changes in Lamin B1 DamID signals upon CRISPRa activation of NLGN1 (a) and SOX6 (b). Control cells were treated either without sgRNA or with one of various sgRNAs targeting promoters on different chromosomes. Vertical green dotted lines mark the position of the sgRNA target sequence."
NCBI gene IDs:
"NLGN1: 22871
SOX6: 55553"
Figure legend: "A-C CRISPRa activation of ADAM22 (a), ABCB1 (b) and PTN (c) in human RPE-1 cells. Top panels visualize DamID data similar to Figure 2a-b. Middle panels show maps of replication timing at the same resolution and in the same plotting style as panels, except that different colors are used as indicated. Bottom panels show gene track, with activated gene highlighted in green."
NCBI gene IDs:
"ABCB1: 5243
ADAM22: 53616
PTN: 5764"
Figure legend: "Changes in NL interactions after CRISPRa activation of MLK4 in human RPE-1 cells. Visualization of DamID data as in Figure 2a-b."
NCBI gene IDs:
"MLK4: 84451"
Figure legend: "(a) DamID domainograms of NL interactions after activation of CCSER1, GRID2, or both. All data are from human RPE-1 cells."
NCBI gene IDs:
"CCSER1: 401145
GRID2: 2895"
Figure legend: "(b) DamID domainograms after activation of ADAM22, ABCB1, or the genes ABCB4, ABCB1, ADAM22 and RUNC3B simultaneously. All data are from human RPE-1 cells. DamID data of activation of ADAM22 and ABCB1 alone are same as in Figure 4a, b."
NCBI gene IDs:
"ABCB1: 5243
ABCB4: 5244
ADAM22: 53616
RUNC3B: 154661"
Figure legend: "(a) DamID profiles of the 129Sv allele of the Morc1 locus with deletions of the promoters of Morc, Morc1 and DppA2 (deletions marked by yellow vertical boxes), and in control cells. (b) Same as (a), but for the non-mutated Cast alleles. "
NCBI gene IDs:
"DppA2: 73703
Morc1: 17450
Morc: 17450"
Figure legend: "(c-d) Same design as (a-b), but with only a single mono-allelic deletion of the Morc promoter (vertical yellow box)."
NCBI gene IDs:
"Morc: 17450"
Figure legend: "(e-f) Effect of PAS insertion on NL interactions of Cobl gene locus. Same design as (a-b) but with insertion of a PAS (located at red triangle and vertical dotted line) that truncates the 129Sv allele of the Cobl transcription unit. 129Sv allele is shown in (e), non-mutated Cast allele in (f). Clones with Morc1 locus mutations (each assayed in four independent biological replicates) served collectively as control in (e-f)"
NCBI gene IDs:
"Cobl: 12808
Morc1: 17450"
Figure legend: "Relative 2-hydroxyglutarate (2-HG) production level based on targeted CE-MS metabolomics comparing among WT and GR cells (left panel: T24, right panel: UMUC3). The data are shown as the means ± SDs (n=3, biological replicates) and were analyzed by Student's t test. *p&lt;0.05, **p&lt;0.01. Relative mRNA expression levels of IDH2 and EGLN1 genes compared between WT and GR cells (left panel: T24, right panel: UMUC3). The expression levels were analyzed by Student's t test and plotted relative to expression levels in WT cells. The data are shown as the means ± SEs (n=3, biological replicates. *p&lt;0.05, ***p&lt;0.001."
NCBI gene IDs:
"EGLN1: 54583
IDH2: 3418"
Figure legend: "Western blot analysis of Hif-1α, GLUT1, G6PD, TIGAR, TKT, CTPS1, PKM2, LDHA, and β-actin after transfection with NTC and IDH2-targeting (siHIF1A#1 and #2) siRNAs (left: T24GR, right: UMUC3GR)."
NCBI gene IDs:
"HIF1A: 3091
IDH2: 3418"
Figure legend: "Relative mRNA expression levels of IDH2, GLUT1, G6PD, TIGAR, TKT, CTPS1, PKM2, and LDHA in cells transfected with siIDH2#1 and #2 compared with cells transfected with siNTC (upper: T24GR, lower: UMUC3GR). The data are shown as the means ± SEs (n=3, biological replicates) and were analyzed by Student's t test, and plotted relative to expression levels in GR cells transfected with siNTC. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001.   Western blot analysis of IDH2, PHD2, HIF-1α, G6PD, GLUT1, TIGAR, TKT, CTPS1, PKM2, LDHA, and β-actin after transfection with NTC and IDH2-targeting (siIDH2#1 and #2) siRNA (left: T24GR, right: UMUC3GR).  "
NCBI gene IDs:
"CTPS1: 1503
G6PD: 2539
GLUT1: 10755
IDH2: 3418
LDHA: 3939
PKM2: 5315
TIGAR: 57103
TKT: 7086"
Figure legend: "Graph showing the viability of GR cells exposed to various concentrations of GEM for 48 hr after transfection with NTC and IDH2-targeting (siIDH2#1 and #2) siRNA (left: T24GR, right: UMUC3GR) The data are shown as the means ± SDs (n=3, biological replicates)."
NCBI gene IDs:
"IDH2: 3418"
Figure legend: "Relative mRNA expression levels of IDH2, EGLN1, Hif-1α, G6PD, TIGAR, TKT, and CTPS1 between J82 wild-type cells (J82WT) and J82 cells with IDH2 overexpression (J82-IDH2ox cells). The data are shown as the means ± SEs (n=3, biological replicates). The expression levels were analyzed by Student's t test and are plotted relative to the expression levels in WT cells. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CTPS1: 1503
EGLN1: 54583
G6PD: 2539
Hif-1α: 3091
IDH2: 3418
TIGAR: 57103
TKT: 7086"
Figure legend: "Western blot analysis of IDH2, PHD2, Hif-1α, G6PD, TIGAR, TKT, CTPS1, and β-actin in J82WT and J82-IDH2ox cells."
NCBI gene IDs:
"IDH2: 3418"
Figure legend: "Graph showing the viability of J82WT and J82-IDH2ox cells exposed to various concentrations of GEM for 48 hr. The data are shown as the means ± SDs (n=3, biological replicates) and were analyzed by Student's t test. ***p&lt;0.001."
NCBI gene IDs:
"IDH2: 3418"