EMBO/SourceData huggingface dataset:
Figure legend: "B Western blot analysis of mtDNA-encoded proteins in PLC cells stably expressing NTC or shPHGDH and empty vector (EV) or PLVX-PHGDH. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: 
PHGDH: 26227"
Figure legend: "C PLC cells with endogenous PHGDH knockdown were further infected with viruses expressing EV or PLVX-PHGDH prior to western blot analysis of mtDNA-encoded proteins. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: 
PHGDH: 26227"
Figure legend: "D PLC cells expressing NTC or shPHGDH were treated with 100 μg/ml cycloheximide (CHX) for 20 min prior to puromycin treatment for 15 min. Purified mitochondrial lysates were subjected to a SUnSET assay with an anti-puromycin antibody to measure the actively translated polypeptide chains. Western blot analysis of PHGDH confirmed the knockdown efficiency of the PHGDH shRNAs. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: 26227"
Figure legend: "F PLC cells with or without PLVX-Flag-PHGDH overexpression were treated with DMSO or 50 μM chloramphenicol (CAP) in combination with 50 μM thiamphenicol (TAP) for 24 h prior to SUnSET assays. The assays were performed using whole-cell lysate."
NCBI gene IDs:
"Flag: 
PHGDH: "
Figure legend: "G PLC cells with or without PLVX-PHGDH overexpression were treated with DMSO or 50 μM CAP in combination with 50 μM TAP for 24 h prior to western blot analysis of mtDNA-encoded proteins. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: "
Figure legend: "H The translation efficiency of mtDNA-encoded proteins was determined by a SUnSET assay in PLC cells with endogenous PHGDH knockdown that were further infected with viruses expressing EV, wild-type PHGDH, or the catalytically inactive PHGDH mutant PHGDHR236E as indicated. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: 
PHGDH: 26227"
Figure legend: "I mtDNA-encoded proteins were analysed by western blotting in PLC cells overexpressing EV, wild-type PHGDH, or PHGDHR236E. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: "
Figure legend: "A HEK293T cells were transfected with Flag-tagged PHGDH in combination with HA-tagged EV, mtIF2, mtIF3, mtEFTu, mtEFG1, mtEFG2, or mtRRF. Co-immunoprecipitation (Co-IP) was performed with an anti-Flag antibody prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
mtEFG1: 
mtEFG2: 
mtEFTu: 
mtRRF: 
PHGDH: 
mtIF2: 4528
mtIF3: 219402"
Figure legend: "B HEK293T cells were transfected with HA-tagged PHGDHWT or PHGDHR236E in combination with Flag-EV or Flag-mtEFG2, and Co-IP was performed with an anti-Flag antibody prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
mtEFG2: 
PHGDH: "
Figure legend: "C HEK293T cells were transfected with HA-tagged PHGDHWT or PHGDHR236E in combination with Flag-EV or Flag-ANT2, and Co-IP was performed with an anti-Flag antibody prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
PHGDH: 
ANT2: 54978"
Figure legend: "D HEK293T cells were transfected with HA-mtEFG2 in combination with Flag-EV or Flag-ANT2, and Co-IP was performed with an anti-Flag antibody prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
mtEFG2: 
ANT2: 54978"
Figure legend: "E GST pull-down of His-PHGDHWT and His-PHGDHR236E by GST-ANT2 using proteins purified in E. coli, followed by western blot analysis with anti-His and anti-GST antibodies."
NCBI gene IDs:
"GST: 
His: 
PHGDH: 
ANT2: 54978"
Figure legend: "F GST pull-down of His-mtEFG2 by GST-ANT2 using proteins purified in E. coli, followed by western blot analysis with anti-His and anti-GST antibodies."
NCBI gene IDs:
"GST: 
His: 
mtEFG2: 
ANT2: 54978"
Figure legend: "G PLC cells stably expressing Flag-mtEFG2 and HA-PHGDH were further infected with viruses expressing NTC or shANT2, and then cell lysates were immunoprecipitated with an anti-Flag antibody or IgG prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
mtEFG2: 
PHGDH: 
ANT2: 54978"
Figure legend: "H PLC cells overexpressing PHGDH were further infected with viruses expressing NTC or shANT2. The translation efficiency was measured by a SUnSET assay."
NCBI gene IDs:
"PHGDH: 
ANT2: 54978"
Figure legend: "I PLC cells overexpressing PLVX-PHGDH were further infected with viruses expressing NTC or shANT2. The expression of mtDNA-encoded proteins was determined by western blotting. β-Actin was used as the loading control."
NCBI gene IDs:
"PHGDH: 
ANT2: 54978"
Figure legend: "PLC cells overexpressing PHGDH were further infected with viruses expressing NTC or shmtEFG2. The translation efficiency was measured by a SUnSET assay."
NCBI gene IDs:
"mtEFG2: 
PHGDH: "
Figure legend: "K PLC cells overexpressing pSin-3xFlag-PHGDH were further infected with viruses expressing NTC or shmtEFG2. The expression of mtDNA-encoded proteins was determined by western blotting. β-Actin was used as the loading control."
NCBI gene IDs:
"Flag: 
mtEFG2: 
PHGDH: "
Figure legend: "A HEK293T cells were transfected with HA-mtEFG2 in combination with Flag-EV or Flag-mtRRF, and Co-IP was performed with an anti-Flag antibody prior to western blot analysis."
NCBI gene IDs:
"Flag: 
HA: 
mtEFG2: 
mtRRF: "
Figure legend: "B PLC cells stably expressing Flag-mtEFG2 and HA-mtRRF were further infected with viruses expressing NTC or shPHGDH, and then cell lysates were immunoprecipitated with anti-Flag antibody or IgG prior to western blot analysis."
NCBI gene IDs:
"PHGDH: 26227"
Figure legend: "C PLC cells stably overexpressing mtEFG2 were further infected with viruses expressing NTC or shPHGDH. The translation efficiency was measured by a SUnSET assay."
NCBI gene IDs:
"mtEFG2: 
PHGDH: 26227"
Figure legend: "D PLC cells stably overexpressing mtEFG2 were further infected with viruses expressing NTC or shPHGDH. mtDNA-encoded proteins were analysed by western blot. β-Actin was used as the loading control."
NCBI gene IDs:
"mtEFG2: 
PHGDH: 26227"
Figure legend: "F Binding of mtDNA-encoded mRNA by endogenous MRPL44 (representing mitochondrial ribosomes) was evaluated by RNA immunoprecipitation (RIP) in PLC cells with PHGDH knockdown."
NCBI gene IDs:
"PHGDH: 26227"
Figure legend: "G The same amount of mitochondria from PLC cells expressing NTC or shPHGDH treated with CAP and TAP were loaded onto the sucrose density gradient. After centrifugation, all fractions were subjected to western blot analysis of MRPS35, MRPL44, and MRPL48 proteins. RNA concentrations of ND6 were measured."
NCBI gene IDs:
"ND6: 4541
PHGDH: 26227"
Figure legend: "A PLC cells overexpressing EV, PHGDHWT, or PHGDHR236E were further infected with viruses expressing NTC or shmtEFG2. The OCR was measured after successive injections of oligomycin (oligo), FCCP, and antimycin A/rotenone (AA/Rot). Basal respiration, ATP-linked respiration, and maximal respiration were analysed with the OCR curve."
NCBI gene IDs:
"mtEFG2: 
PHGDH: "
Figure legend: "B PLC cells overexpressing EV, PHGDHWT, or PHGDHR236E were further infected with viruses expressing NTC or shmtEFG2 prior to western blot analysis. β-Actin was used as the loading control (left). Cell growth curves were constructed by cell counting with trypan blue exclusion (right)."
NCBI gene IDs:
"mtEFG2: 
PHGDH: "
Figure legend: "C PLC cells overexpressing EV, PHGDHWT, or PHGDHR236E were further treated with H2O or tigecycline prior to western blot analysis. β-Actin was used as the loading control (left). Cell growth curves were determined by cell counting with trypan blue exclusion (right)."
NCBI gene IDs:
"PHGDH: "
Figure legend: "D-F PLC cells overexpressing EV or PHGDH were further infected with viruses expressing NTC or shmtEFG2. Then, these cells were subcutaneously injected into male nude mice (n = 5 in each group). Tumour sizes were measured beginning 14 days after inoculation (D). At the end of the experiment, the tumours were excised (E), and PHGDH and mtEFG2 expression in tumour lysates was analysed by western blotting (F)."
NCBI gene IDs:
"mtEFG2: 
PHGDH: "
Figure legend: "G-I PLC cells overexpressing EV or PHGDH were subcutaneously injected into male nude mice (n = 5 in each group). Mice were intraperitoneally injected with 100 mg/kg tigecycline daily beginning 14 days after inoculation. Tumour sizes were measured beginning 14 days after inoculation (G). At the end of the experiment, the tumours were excised (H), and PHGDH expression in tumour lysates was analysed by western blotting (I)."
NCBI gene IDs:
"PHGDH: "
Figure legend: "WT or SLC15A4feeble BMDCs unstimulated or infected with flagellin-expressing STm  Cell pellets collected at the indicated time points after STm  infection were lysed, fractionated by SDS-PAGE and immunoblotted  for caspase-1 (E, F) (E Representative immunoblots  showing pro-caspase-1  (pro-casp.-1) and cleaved p20 (casp.-1 p20) bands (E) (F, Quantification of band intensities for caspase-1 p20 normalized to pro-caspase-1 and actin  (E) from three independent experiments are shown as fold change (F Data information: Data represent mean ± SD. * p&lt;0.05; **p&lt;0.01; ***p&lt;0.001. Two-tailed Student's t-test."
NCBI gene IDs:
"SLC15A4: 100561"
Figure legend: "WT or SLC15A4feeble BMDCs  unstimulated or infected with flagellin-expressing STm  Cell pellets collected at the indicated time points  after STm  infection were lysed, fractionated by SDS-PAGE and immunoblotted  for GSDMD  or LC-3 G). Representative immunoblots  showing GSDMD  full length (GSDMD  FL), cleaved GSDMD  N-terminal fragment (GSDMD  N-ter), LC3-II and LC3-I  bands in cell lysates (G). H, Quantification of band intensities for GSDMD  N-ter normalized to actin  (G) and LC3-II  normalized to LC3-I and actin  (H) from three independent experiments are shown as fold change , H) Data information: Data represent mean ± SD. * p&lt;0.05; **p&lt;0.01; ***p&lt;0.001. Two-tailed Student's t-test."
NCBI gene IDs:
"SLC15A4: 100561"
Figure legend: "Intestinal CD11c+ DCs  were isolated from colons of WT and SLC15A4feeble mice on day 16 and cultured overnight. B. Cell pellets from three independent experiments were immunoblotted for LC3 and actin. Upper panel. Representative immunoblot. Lower panel. Quantification of band intensities for LC3-II normalized to LC3-I  and actin  are shown as fold induction relative to samples from untreated mice. Note that LC3-I  signal from ex vivo samples is almost undetectable. Data information: Mean is indicated. **p&lt;0.01. Mann-Whitney non-parametric statistical test."
NCBI gene IDs:
"SLC15A4: 100561"
Figure legend: "WT or  SLC15A4feeble BMDCs expressing ASC-GFP  and mCherry-LC3  (A, B were infected with flagellin-expressing STm and fixed A. Cells were analyzed by  fluorescence microscopy. Representative images showing ASC  speck (green) relative to mCherry-LC3  (red) in two infected WT and SLC15A4feeble DCs  each. B. Quantification of LC3  fluorescence per unit area in a radius of 1 μm surrounding the ASC  speck in 20 cells per cell type in each of 3 independent experiments. N, nucleus. Corresponding DIC images show nuclear  position. Scale bar, 8 μm. Data information: Data represent mean ± SD. **p&lt;0.01. Two-tailed Student's t-test."
NCBI gene IDs:
"GFP: 
mCherry: 
LC3: 440738///84557///81631
ASC: 29108
SLC15A4: 100561"
Figure legend: "WT or SLC15A4feeble BMDCs non-transduced (C) were infected with flagellin-expressing STm  and fixed C. Cells were stained  for endogenous ASC  and p62, labeled with DAPI  and analyzed by fluorescence microscopy. Representative images showing ASC  speck (red) relative to p62  (green) in two infected WT and SLC15A4feeble DCs  each. Note p62 staining  surrounding ASC  specks in SLC15A4feeble DCs. D. Quantification of perinuclear  (within a radius of 3 μm from the nucleus) ASC  specks in at least 30 cells per cell type in each of 3 independent experiments. N, nucleus. Corresponding DIC  images show nuclear  position. Scale bar, 8 μm. Data information: Data represent mean ± SD. **p&lt;0.01. Two-tailed Student's t-test."
NCBI gene IDs:
"SLC15A4: 100561"
Figure legend: "SLC15A4feeble BMDCs  transduced with SLC15A4-GFP  (E were infected with flagellin-expressing STm  and fixed (E, Cells were stained  for endogenous ASC, labeled with DAPI  and analyzed by fluorescence microscopy. Representative images showing ASC  speck (red) and SLC15A4-GFP  (green) in SLC15A4feeble DCs  together with non-transduced SLC15A4feeble DCs. Note the perinuclear  positioning of ASC  specks in the transduced cells (E). N, nucleus. Dotted white lines, cell outlines. Corresponding DIC  images show nuclear  position. Scale bar, 8 μm."
NCBI gene IDs:
"GFP: 
SLC15A4: 100561
SLC15A4: 121260"
Figure legend: "SLC15A4feeble BMDCs   transduced with SLC15A4-GFP   F, were infected with flagellin-expressing STm   and fixed Quantification of perinuclear   (within a radius of 3 μm from the nucleus) and non-perinuclear ASC   specks in at least 30 cells per cell type in each of three independent experiments (F). Data information: Data represent mean ± SD. **p&lt;0.01. Two-tailed Student's t-test."
NCBI gene IDs:
"GFP: 
SLC15A4: 100561
SLC15A4: 121260"
Figure legend: "SLC15A4feeble BMDCs  transduced with SLC15A4-GFP  G) were infected with flagellin-expressing STm  and lysed (G) 1 h after infection. G. Representative immunoblots  showing pro-caspase-1 (pro-casp.-1) and cleaved p20 (casp.-1 p20), LC3-II and LC3-I, and actin  bands."
NCBI gene IDs:
"GFP: 
SLC15A4: 100561
SLC15A4: 121260"
Figure legend: "WT or SLC15A4feeble BMDCs transduced with constitutively active RagB  (RagBQ99L) or metap2  (control) were infected with flagellin-expressing STm. (A, B). Cell pellets collected 1 h after STm  infection were lysed, fractionated by SDS-PAGE and immunoblotted  for phospho  (P) and total mTOR, ULK1  kinase, p70  kinase and S6, FLAG  and actin. Representative immunoblots. Non-specific band right above FLAG-RagBQ99L is indicated with an asterisk (A). Quantification of band intensities for P-mTOR  normalized to total mTOR, P-ULK1  normalized to total ULK1, P-p70  normalized to total p70  and P-S6  normalized to S6, in transduced cells from 3 independent experiments are shown as fold change relative to WT control (B). Data information: Data represent mean ± SD. **p&lt;0.01; ***p&lt;0.001. Two-tailed Student's t-test."
NCBI gene IDs:
"metap2: 10988
RagB: 10325
SLC15A4: 100561"
Figure legend: "WT or SLC15A4feeble BMDCs  transduced with constitutively active RagB  (RagBQ99L) or metap2  (control) (E, Cell pellets were probed for caspase-1, GSDMD, actin  (E, Representative immunoblots  showing pro-caspase-1  (pro-casp.-1) and cleaved p20 (casp.-1  p20), GSDMD  full length (GSDMD  FL) and cleaved GSDMD  N-terminal fragment (GSDMD  N-ter) bands."
NCBI gene IDs:
"metap2: 10988
RagB: 10325
SLC15A4: 100561"
Figure legend: "WT or SLC15A4feeble BMDCs  transduced with non-target control, ATG5  or ATG7  shRNAs G) G). Cell pellets were probed for caspase-1, GSDMD, actin  , ATG5  and ATG 7 (G). Representative immunoblots  showing pro-caspase-1 (pro-casp.-1) and cleaved p20 (casp.-1 p20), GSDMD  full length (GSDMD  FL) and cleaved GSDMD  N-terminal fragment (GSDMD  N-ter) bands."
NCBI gene IDs:
"ATG5: 11793
ATG7: 74244
SLC15A4: 100561"
Figure legend: "WT or SLC15A4feeble BMDCs  transduced with constitutively active RagB  (RagBQ99L) or metap2  (control) H, I) and mcherry-LC3  (H, I), (H, I). Cells were stained  for endogenous ASC, labeled with DAPI  and analyzed by fluorescence microscopy. Representative images showing ASC  speck (green) and LC3  (red) in SLC15A4feeble DCs  (H). Quantification of perinuclear  (within a radius of 3 μm from the nucleus) in at least 30 cells per cell type in each of three independent experiments (I). N, nucleus. Corresponding DIC  images show nuclear  position. Scale bar, 8 μ Data information: Data represent mean ± SD. **p&lt;0.01; ***p&lt;0.001. Two-tailed Student's t-test."
NCBI gene IDs:
"mcherry: 
LC3: 440738///84557///81631
metap2: 10988
RagB: 10325
SLC15A4: 100561"
Figure legend: "B Western blot analysis (probing with an anti-FLAG mAb) to detect cleavage of the artificial substrate by wild-type (WT)/inactive (S201A) GlpG encoded on pBAD33 with/without arabinose (Ara.). rhomboid substrates that are uncleaved, cleaved by GlpG are marked by black, red, arrows, respectively. Controls, empty pBAD33 (empty)"
NCBI gene IDs:
"GlpG: "
Figure legend: "C Western blot analysis to detect cleavage of the artificial substrate by wild-type (WT)/modified (S133A or H187A) Rhom7 encoded on pBAD33 with/without arabinose (Ara.). , rhomboid substrates that are uncleaved, cleaved by Rhom7, are marked by black, and blue arrows, respectively. Controls, empty pBAD33 (empty) and wild-type S. sonnei (Ss)."
NCBI gene IDs:
"Rhom7: "
Figure legend: "D Activity of Rhom7 with/without its 7th TMD and/or C-terminal domain. rhomboid substrates that are uncleaved, cleaved by Rhom7, are marked by black, , and blue arrows, respectively. Controls, empty pBAD33 (empty) and wild-type S. sonnei (Ss)."
NCBI gene IDs:
"Rhom7: "
Figure legend: "C Western blot analysis of candidate substrates reproducibly cleaved by GlpG from the screen. Data information: In (C rhomboid substrates that are uncleaved, cleaved by GlpG are marked by black, red, arrows, respectively. Controls, inactive enzymes and wild-type S. sonnei (Ss)."
NCBI gene IDs:
"GlpG: "
Figure legend: "D Western blot analysis of candidate substrates reproducibly cleaved by Rhom7 from the screen. Data information: In D) rhomboid substrates that are uncleaved, cleaved by Rhom7, are marked by black, red, and blue arrows, respectively. Controls, inactive enzymes and wild-type S. sonnei (Ss)."
NCBI gene IDs:
"Rhom7: "
Figure legend: "Western blot analysis (probing with an anti-FLAG mAb) to detect cleavage of HybO with (+)/without (-) chromosomal (native) or pBAD33-encoded rhomboids (plasmid). homboid substrates that are uncleaved, are marked by black, rrows, ild-type S. sonnei, Ss. Wild-type (WT)/inactive (SAHA: alanine substitution of the catalytic serine and histidine residues) enzymes were pBAD33-encoded (plasmid) in S. sonneiΔglpGΔrhom7."
NCBI gene IDs:
"glpG: 
rhom7: "
Figure legend: "Western blot analysis to detect cleavage of HybA with (+)/without (-) chromosomal (native) or pBAD33-encoded rhomboids (plasmid). rhomboid substrates that are uncleaved, cleaved by GlpG, or by Rhom7, are marked by black, red, and blue arrows, respectively. Wild-type S. sonnei, Ss. Wild-type (WT)/inactive (SAHA: alanine substitution of the catalytic serine and histidine residues) enzymes were pBAD33-encoded (plasmid) in S. sonneiΔglpGΔrhom7."
NCBI gene IDs:
"glpG: 
rhom7: 
GlpG: 
Rhom7: "
Figure legend: "Western blot analysis to detect cleavage of HybAG296F by endogenous or pBAD33-encoded rhomboid. Wild-type S. sonnei, Ss. rhomboid substrates that are uncleaved, cleaved by GlpG, or by Rhom7, re marked by black, red, and blue arrows, espectively. ild-type S. sonnei, Ss. Wild-type (WT)/inactive (SAHA: alanine substitution of the catalytic serine and histidine residues) enzymes were pBAD33-encoded (plasmid) in S. sonneiΔglpGΔrhom7."
NCBI gene IDs:
"glpG: 
rhom7: 
GlpG: 
Rhom7: "
Figure legend: "Western blot analysis to detect cleavage of HybAP300A by endogenous or pBAD33-encoded rhomboid. rhomboid substrates that are uncleaved, cleaved by GlpG, or by Rhom7, are marked by black, red, and blue arrows, respectively. Wild-type S. sonnei, Ss. Wild-type (WT)/inactive (SAHA: alanine substitution of the catalytic serine and histidine residues) enzymes were pBAD33-encoded (plasmid) in S. sonneiΔglpGΔrhom7."
NCBI gene IDs:
"glpG: 
rhom7: 
GlpG: 
Rhom7: "
Figure legend: "A Bacterial two-hybrid analysis with HybA and/or HybB fused chromosomally with the T25 and T18 domains of B. pertussis CyaA, respectively in the absence of endogenous CyaA. Bacteria were grown on LB agar containing 20 µg/ml X-gal at 37°C for 14 hours in the presence/absence of O2. Scale bar, 1 cm."
NCBI gene IDs:
"CyaA: 
CyaA: 2664492"
Figure legend: "B Western blot analysis to detect HybA cleavage in S. sonneiΔrhom7 by wild-type (+) or inactive (S201A) GlpG expressed chromosomally (native) or from pUC19 (plasmid) with (+)/without (-) HybB. HybA was expressed from its native locus or a plasmid (pHybA). HybA that is uncleaved or cleaved by GlpG is marked by black and red arrows, respectively."
NCBI gene IDs:
"rhom7: 
GlpG: 
HybA: 
HybB: "
Figure legend: "C Quantification of the ratio of cleaved/uncleaved HybA in strains with (+) or without (-) HybB. mean ± S.D. of three experiments. *, p&lt;0.05; ****, p&lt;0.0001 (One-way ANOVA)."
NCBI gene IDs:
"HybB: "
Figure legend: "D Western blot analysis to detect GlpG-mediated cleavage of native tagged HybA at indicated times after blocking protein translation by the addition of chloramphenicol at T0 in the absence of HybB (-). , HybA that is uncleaved or cleaved by GlpG is marked by black and red arrows, respectively."
NCBI gene IDs:
"GlpG: "
Figure legend: "E Western blot analysis to detect GlpG-mediated cleavage of C-terminally sfCherry-3xFLAG tagged HybA at times after blocking protein translation by the addition of chloramphenicol at T0 in the presence of HybB (+). , HybA that is uncleaved or cleaved by GlpG is marked by black and red arrows, respectively."
NCBI gene IDs:
"GlpG: 
HybB: "
Figure legend: "F Growth of bacteria lacking hydrogenases (ΔhyaA-F ΔhycE +/- ΔhybO-G) or GlpG (ΔglpG), or expressing uncleavable HybB (hybAG296F) in 5% H2"
NCBI gene IDs:
"glpG: 
GlpG: 
hyaA-F: 
hybA: 
HybB: 
hybO: 
hycE: "
Figure legend: "H S. sonnei growth in 10% H2 with plasmid expressed wild-type or non-functional (pglpGSAHA) GlpG."
NCBI gene IDs:
"glpG: 
GlpG: "
Figure legend: "B Western blot analysis (probing with an anti-FLAG mAb) to detect cleavage of FdoH cleavage in S. sonneiΔrhom7 with (+)/without (-) FdoI. rhomboid substrates that are uncleaved, cleaved by GlpG are marked by black, red arrows, respectively."
NCBI gene IDs:
"rhom7: 
FdoI: 
GlpG: "
Figure legend: "C Western blot analysis to detect cleavage of FdnH in S. sonneiΔglpG with (+) or without (-) chromosomal Rhom7 (native), or wild-type (+)/inactive (SAHA) Rhom7 expressed from pBAD33 in S. sonneiΔglpGΔrhom7 with (+) or without (-) FdnI. rhomboid substrates that are uncleaved, cleaved by Rhom7, are marked by black, and blue arrows, respectively."
NCBI gene IDs:
"glpG: 
rhom7: 
FdnI: 
Rhom7: "
Figure legend: "E Western blot analysis to detect cleavage of wild-type (WT) or uncleavable (P300A) HybA by active (+) or inactive (S201A) chromosomal GlpG in the presence (+) or absence (-) of HybB. rhomboid substrates that are uncleaved, cleaved by GlpG are marked by black, red arrows, respectively."
NCBI gene IDs:
"GlpG: 
HybB: "
Figure legend: "F Western blot analysis to detect cleavage of wild-type (WT) or modified (P259A) FdoH by active (+) or inactive (S201A) chromosomal GlpG in the presence (+) or absence (-) of FdoI. rhomboid substrates that are uncleaved, cleaved by GlpG are marked by black, red arrows, respectively."
NCBI gene IDs:
"FdoI: 
GlpG: "
Figure legend: "G Western blot analysis to detect cleavage of wild-type (WT) or modified (P259A) FdnH with active (+) or inactive (SAHA) Rhom7 expressed from pBAD33 with (+) or without (-) FdnI. rhomboid substrates that are uncleaved, cleaved by Rhom7, are marked by black, blue arrows, respectively."
NCBI gene IDs:
"FdnI: 
Rhom7: "
Figure legend: "A Western blot analysis (probing with an anti-V5 mAb) to detect degradation of N-terminally V5-tagged wild-type (WT) or modified (P300A) HybA in S. sonneiΔrhom7 chromosomally expressing wild-type (+) or inactive (S201A) GlpG with (+) or without (-) HybB. Rhomboid substrates that are uncleaved, cleaved by GlpG, , are marked by black, red, arrows, respectively. Degradation products post rhomboid cleavage are marked by green arrows."
NCBI gene IDs:
"rhom7: 
GlpG: 
HybB: "
Figure legend: "B Degradation of V5-tagged HybA at times after blocking protein translation at T0 in the presence (+) or absence (-) of HybB. Rhomboid substrates that are uncleaved, cleaved by GlpG, black, red, arrows, respectively. Degradation products post rhomboid cleavage are marked by green arrows."
NCBI gene IDs:
"GlpG: 
HybB: "