EMBO/SourceData huggingface dataset:
Figure legend: "A and B, ChIP-qPCR assay of  HDA15's (A) and NF-YC9's (B) residence at target promoters in presence or absence of TOC1  and PRR5. GFP-HDA15 and GFP-NF-YC9 driven by 35S promoter were transiently expressed in protoplasts isolated from  Col-0, toc1  single and prr5 toc1 double mutant grown in SD condition. Results were normalized by % input of PIL1 coding region (PIL1 CDS). Data from at least 3 independent experiments were averaged, error bars indicate SEM. Asterisks indicate significant differences (* p&lt;0.05, ** p&lt;0.001, *** p&lt;0.0001, Student's t test)."
NCBI gene IDs:
"PIL1: 819311
PRR5: 832518
prr5: 832518
toc1: 836259
TOC1: 836259"
Figure legend: "C and D, Dual  luciferase assay   of the repression activity of  GFP-TOC1 and  GFP-5X on promoter of AT5G02580 (C) and CDF5 (D) in protoplasts of toc1 and hda15toc1 (h15t1) mutants.  AT5G02580:LUC,  CDF5:LUC reporters and the effectors (empty GFP, GFP-TOC1, and GFP-5X) were co-transfected to toc1 and hda15 toc1 protoplasts with 35S:Renilla-LUC as internal control. Data are means ± SEM (n = 5 biological trials) relative to empty  GFP which is set as 1. Different letters indicate statistically significant differences (student's t test, p&lt;0.05)."
NCBI gene IDs:
"GFP: 
LUC: 
CDF5: 843293
AT5G02580: 831095
hda15: 821382
TOC1: 836259
toc1: 836259"
Figure legend: "C, ChIP-qPCR assay of native promoter TOC1-GFP and 5X-GFP binding to the target promoters at ZT14 in toc1  vs. prr5 toc1 (p5t1) mutant. 3-d-old SD-grown  seedlings were harvested at ZT14, TOC1  and 5X were  immunoprecipitated by α-GFP. Data from 3 independent trials were averaged, error bars indicate SEM. Asterisks indicate significant differences (Student's t test; * p&lt;0.05), n.s= not significant."
NCBI gene IDs:
"prr5: 832518
toc1: 836259"
Figure legend: "D, Expression of PIL1, AT5G02580 and CDF5 in 3-d-old SD-grown  seedlings at ZT18. Data from 3 biological trials were averaged. Error bars indicate SEM. Different letters denote statistically significant differences based on Wilcoxon test (p&lt;0.05)."
NCBI gene IDs:
"CDF5: 843293
AT5G02580: 831095
PIL1: 819311"
Figure legend: "A Detection of the RBPJ/L3MBTL3 interaction using the yeast two-hybrid (Y2H) assay. In this Y2H experiment, RBPJ is fused to the GAL4 DNA-binding (DB) domain and L3MBTL3 is fused to the GAL4 activation domain (AD). The DB-RBPJ and AD-L3MBTL3 fusion proteins interact with each other, leading to the activation of the ADE2 and HIS3 reporter genes and allowing yeast cells to grow on selective media lacking adenine or histidine. The six Y2H controls were previously described (Dreze et al, 2010). The experiment was independently replicated thrice."
NCBI gene IDs:
"ADE2: 854295
HIS3: 854377"
Figure legend: "B/C NOTCH1 ICD outcompetes L3MBTL3 for binding to RBPJ in a dose-dependent manner. IPs were performed in CRISPR/Cas9-mediated L3MBTL3 knockout (KO) HEK293T cells. (B) SBP-FLAG-RBPJ and HA-L3MBTL3-Δ(SAM) in the presence of an increasing amount of HA-NOTCH1 ICD. The L3MBTL3-Δ(SAM) mutant construct was used instead of the L3MBTL3 WT construct in order to allow the analysis of both NOTCH1 ICD and L3MBTL3 proteins in the same Western blot. CRISPR/Cas9 sg-L3MBTL3-resistant plasmids were used to express HA-L3MBTL3-Δ(SAM). The experiment was independently replicated thrice. WB: Western blot; IP: immuno-precipitation."
NCBI gene IDs:
"L3MBTL3: 84456"
Figure legend: "B/C NOTCH1 ICD outcompetes L3MBTL3 for binding to RBPJ in a dose-dependent manner. IPs were performed in CRISPR/Cas9-mediated L3MBTL3 knockout (KO) HEK293T cells. (C) SBP-FLAG-RBPJ and HA-NOTCH1 ICD in the presence of an increasing amount of HA-L3MBTL3-Δ(SAM). The L3MBTL3-Δ(SAM) mutant construct was used instead of the L3MBTL3 WT construct in order to allow the analysis of both NOTCH1 ICD and L3MBTL3 proteins in the same Western blot. CRISPR/Cas9 sg-L3MBTL3-resistant plasmids were used to express HA-L3MBTL3-Δ(SAM). The experiment was independently replicated thrice. WB: Western blot; IP: immuno-precipitation."
NCBI gene IDs:
"L3MBTL3: 84456"
Figure legend: "A De-repression of Notch target genes upon RBPJ knockdown. Shown are means ±s.d. of quadruplicate experiments. [*] P&lt;0.05, [**] P&lt;0.01, NS: Not Significant; one-way ANOVA model on log-transformed data. Inset: Western blot analysis validates the shRNA-mediated depletion of RBPJ."
NCBI gene IDs:
"RBPJ: 3516"
Figure legend: "B De-repression of Notch target genes in L3MBTL3 KO U87-MG cells. Shown are means ±s.d. of quadruplicate experiments. [**] P&lt;0.01, NS: Not Significant; two-sample T-test on log-transformed data. Inset: Western blot analysis validates the CRISPR/Cas9-mediated KO of L3MBTL3."
NCBI gene IDs:
"L3MBTL3: 84456"
Figure legend: "C RBPJ and L3MBTL3 co-localize at the proximal Notch-responsive elements of Notch target genes. Shown are means ±s.d. of triplicate ChIP experiments.In panels C, D and F: distance in base pairs (bp) relative to the transcriptional start site (TSS) is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"Chrom8: "
Figure legend: "D L3MBTL3 occupancy at the proximal Notch-responsive elements of Notch target genes decreases upon RBPJ knockdown. Shown are means ±s.d. of triplicate ChIP experiments.In panels C, D and F: distance in base pairs (bp) relative to the transcriptional start site (TSS) is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"Chrom8: 
RBPJ: 3516"
Figure legend: "E The repressive activity of L3MBTL3 at Notch target genes is RBPJ-dependent. Expression analysis of Notch target genes upon RBPJ knockdown and/or overexpression of L3MBTL3. Shown are means ±s.d. of triplicate experiments. P values were estimated via a one-way ANOVA model on log-transformed data where the difference of differences was tested, which is equivalent to testing the interaction in a two-way ANOVA model. Western blot analysis validates the overexpression of L3MBTL3 and the shRNA-mediated depletion of RBPJ (Appendix Fig S3E). Gene expression analyses of OCT4 was performed as control (Appendix Fig S3F)."
NCBI gene IDs:
"RBPJ: 3516"
Figure legend: "F L3MBTL3 occupancy at the proximal Notch-responsive elements of Notch target genes is dependent on its RBPJ interaction domain. ChIP analyses of HA-L3MBTL3 WT and HA-L3MBTL3-Δ(1-64) occupancy at the proximal Notch-responsive elements of Notch target genes. Shown are means ±s.d. of duplicate experiments measured twice each.In panels C, D and F: distance in base pairs (bp) relative to the transcriptional start site (TSS) is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"Chrom8: "
Figure legend: "G The L3MBTL3-(1-64) domain is required for the downregulation of HES1 and HEY2 in U87-MG cells. Expression analysis of Notch target genes upon overexpression of L3MBTL3 WT, L3MBTL3-Δ(1-64) or LacZ control (Control). Shown are means ±s.d. of triplicate experiments. [*] P&lt;0.05, [**] P&lt;0.01, NS: Not Significant; one-way ANOVA model on log-transformed data."
NCBI gene IDs:
"HES1: 3280
HEY2: 23493"
Figure legend: "A The RBPJ/KDM1A interaction is indirect and occurs via L3MBTL3. IP of HA-KDM1A in the presence of overexpressed V5-L3MBTL3 or V5-L3MBTL3-Δ(1-64) in L3MBTL3 KO U87-MG cells. CRISPR/Cas9 sg-L3MBTL3-resistant plasmids were used to overexpress the L3MBTL3 proteins. The experiment was independently replicated twice."
NCBI gene IDs:
"L3MBTL3: 84456"
Figure legend: "B KDM1A occupancy at the proximal Notch-responsive elements of Notch target genes is L3MBTL3-dependent. ChIP analysis of endogenous KDM1A in L3MBTL3 KO U87-MG cells. Shown are means ±s.d. of duplicate experiments measured twice each.Panels B, C and D: distance in bp relative to the TSS is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"Chrom8: 
L3MBTL3: 84456"
Figure legend: "C KDM1A occupancy at the proximal Notch-responsive elements of Notch target genes is dependent on L3MBTL3 and both its RBPJ interaction and KDM1A interaction domains. ChIP analysis of endogenous KDM1A in L3MBTL3 KO U87-MG cells upon overexpression of L3MBTL3, L3MBTL3-Δ(1-64) or L3MBTL3-Δ(SAM). Control: empty vector. Shown are means ±s.d. of duplicate experiments measured twice each.Panels B, C and D: distance in bp relative to the TSS is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"Chrom8: 
L3MBTL3: 84456"
Figure legend: "D L3MBTL3, but neither L3MBTL3-Δ(1-64) nor L3MBTL3-Δ(SAM), leads to decreasing H3K4me2 at the proximal Notch-responsive element of HES1. ChIP analysis of H3K4me2 at the proximal Notch-responsive element of HES1 upon overexpression of LacZ control (Control), L3MBTL3, L3MBTL3-Δ(1-64) or L3MBTL3-Δ(SAM) in L3MBTL3 KO U87-MG cells. Shown are means ±s.d. of duplicate experiments measured twice each. P values were estimated via a one-way ANOVA on log-transformed data.Panels B, C and D: distance in bp relative to the TSS is indicated below the gene names. Chrom8 was used as negative control (NEG)."
NCBI gene IDs:
"L3MBTL3: 
Chrom8: 
HES1: 3280
L3MBTL3: 84456"
Figure legend: "E L3MBTL3, but neither L3MBTL3-Δ(1-64) nor L3MBTL3-Δ(SAM), represses HES1. Expression analysis of HES1 upon overexpression of LacZ control (Control), L3MBTL3, L3MBTL3-Δ(1-64) or L3MBTL3-Δ(SAM) mutants in L3MBTL3 KO U87-MG cells. Shown are means ±s.d. of triplicate experiments. P values were estimated via a one-way ANOVA on log-transformed data. NS: Not Significant. WB: Western blot; IP: immuno-precipitation. We note that in the context of this experiment, i.e., in the absence of endogenous L3MBTL3, the overexpression of L3MBTL3-Δ(1-64) does not result in the increased expression of HES1, contrasting with the result obtained in Fig 4G, i.e., in the presence of endogenous L3MBTL3. Indeed, as expected, the dominant negative effect of L3MBTL3-(1-64) on endogenous WT L3MBTL3&apos;s ability to repress the expression of Notch target genes can only be observed when WT L3MBTL3 is expressed."
NCBI gene IDs:
"HES1: 3280
L3MBTL3: 84456"
Figure legend: "C Snapshot showing the co-localization of dL(3)mbt and Su(H) at the dNotch (N) locus."
NCBI gene IDs:
"dNotch: 31293"
Figure legend: "F Functional interaction between lag-1/RBPJ and lin-61/L3MBTL3 during C. elegans vulva development. Proportion of animals (n ≥100) displaying a protruding vulva (Pvl) phenotype after RNAi treatment for two generations. Worms were grown at 25°C. Shown are means ±s.d of duplicate experiments. EV: Empty Vector control."
NCBI gene IDs:
"lag-1: 177373
lin-61: 172467"
Figure legend: "H Fluorescence microscopy of the SARS-CoV-2 GFP/ΔN or VSV-GFP  viruses in  Caco-2-N cells pretreated with VitC  (5 mM and 10 mM) for 24 hrs, and then infected with SARS-CoV-2 GFP/ΔN (MOI = 0.1) or VSV-GFP  (MOI = 0.1) viruses for 24 hrs. Data are representative of three biological replicates. Scale bar: 100 µm."
NCBI gene IDs:
"GFP: "
Figure legend: "A  RT-qPCR  analysis of Ace2  mRNA in 2fTGH  cells treated with VitC  (5 mM) as indicated. Data are shown as mean and s.d. of three biological replicates (n = 3). N.S, not significant (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"Ace2: 59272"
Figure legend: "F IP-IB  analysis of ubiquitination  types of Myc-ACE2  in HEK293T  cells cotransfected with Myc-ACE2  and different types of HA-Ub, and then treated with VitC  (5 mM) for 12 hrs. The red dashed contour represents the group of K48-linked ubiquitination  we focused on studying here. Data are representative of three biological replicates."
NCBI gene IDs:
"HA: 
Myc: 
Ub: 
ACE2: 59272"
Figure legend: "G  Western blot  analysis of ACE2  in HEK293T  cells transfected with Flag-USP50  and then treated with CHX  (50 μM) as indicated. Data are representative of three biological replicates."
NCBI gene IDs:
"Flag: 
USP50: 373509"
Figure legend: "K Fluorescence microscopy  of the VSV  virus with a SARS-CoV-2-S gene and a GFP  gene (VSV-Spike) in Usp50+/+ and Usp50-/- HEK293T   cells pretreated with or without VitC  (5 mM) for 12 hrs, followed by infection  with the VSV-Spike  virus (MOI = 0.1) for 24 hrs. Scale bar: 100 µm. The average intensity each unit area was shown as mean and s.d. of three-unit areas (n = 3). N.S, not significant, ***p &lt; 0.001 (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"Usp50: 373509"
Figure legend: "D IP-IB  analysis of ubiquitination  types of Myc-ACE2  in HEK293T  cells cotransfected with Myc-ACE2, Flag-USP50  and different types of HA-Ub. The red dashed contour represents the group of K48-linked ubiquitination  we focused on studying here. Data are representative of three biological replicates."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
Ub: 
ACE2: 59272
USP50: 373509"
Figure legend: "E IP-IB  analysis of K48-Ub  of endogenous ACE2  in HEK293T  cells transfected with shCtrl (-) or shUSP50 (#1, #2). Data are representative of three biological replicates."
NCBI gene IDs:
"USP50: 373509"
Figure legend: "B IP-IB  analysis of Myc-ACE2  K48-linked ubiquitination  in HEK293T  cells cotransfected with Myc-ACE2  (WT or K788R) and HA-K48, together with shCtrl or shUSP50. Data are representative of three biological replicates."
NCBI gene IDs:
"HA: 
Myc: 
ACE2: 59272
USP50: 373509"
Figure legend: "C Western blot analysis of Myc-ACE2  in HEK293T  cells transfected with Myc-ACE2  (WT or K788R) and then treated with CHX  (50 μM) as indicated. Data are representative of three biological replicates."
NCBI gene IDs:
"Myc: 
ACE2: 59272"
Figure legend: "D IP-IB  analysis of K48-Ub  of Myc-ACE2  in HEK293T  cells transfected with Myc-ACE2  (WT or K788R) and then treated with VitC  (5 mM) for 12 hrs, by a specific anti-K48-Ub  antibody. Data are representative of three biological replicates."
NCBI gene IDs:
"Myc: 
ACE2: 59272"
Figure legend: "G ACE2-KO  Caco-2 cells transfected with ACE2  (WT or K788R) were treated with VitC  (5 mM) for 12 hrs, and then were infected with SARS-CoV-2 GFP/ΔN (MOI = 0.1) for 2 hrs. RT-qPCR was used to analyze SARS-CoV-2 RNA  levels. Data are shown as mean and s.d. of three biological replicates (n = 3). N.S, not significant, ***p &lt; 0.001 (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"GFP: 
ACE2: 59272"
Figure legend: "D Western blot analysis of hACE2  levels in lung  tissues of hACE2 mice  (n = 6) administrated with VitC  as (B). Data are representative of three biological replicates."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "G  RT-qPCR  analysis of the SARS-CoV-2-Spike  mRNA levels in lung, kidney, liver  and spleen  tissues of hACE2 mice  treated with VitC  and the VSV-Spike  viruses as (F). All graphs show the mean ± SEM for five individual mice  (n = 5). ***p &lt; 0.001 (two-tailed unpaired Student's t-test)."
NCBI gene IDs:
"ACE2: 59272
Spike: 43740568"
Figure legend: "A.Volcano plot showing the fold change (y-axis) versus adjusted (adj.) p-value (x-axis) of the beta cell  transcriptomes between   chow and HFD-fed mice (16 weeks). Genes highlighted in red or green are based on the thresholds of Log2 fold change &gt; 1 and adj. P value &lt; 0.01 (two-tailed paired student's t-test). Genes with &gt;10 fold upregulated expression are labeled with their name. Pbk gene expression is indicated by the arrow. RNAseq  data was available from https://doi.org/10.1371/journal.pone.0213299.t004."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "L. Pbk KD in INS-1 cells suppresses cell growth. Western blot data showed the decreased Pbk expression level in INS-1 cells with Pbk-targeted shRNA transduction. Cell growth curve was from three independent experimets (n=3). ***P &lt; 0.0001 (Vector:shRNA-#1), *P = 0.0181 (Vector:shRNA-#5) , (Two way ANOVA). M. The role of ectopic expression of Pbk-WT and Pbk-Mut on INS-1 cell growth. Western blot data showed the overexpression of V5-flaged wild type and mutant Pbk in INS-1 cells. Cell growth curve was from three independent experimets (n=3). **P = 0.0069 (Two way ANOVA). ns, not statistically significant difference. "
NCBI gene IDs:
"Pbk: 290326"
Figure legend: "F.  Islet number in each pancreas section on 11-week-old male PbkKI/KI and PbkWT/WT mice (n=4 for each group). *P = 0.0443 (two-tailed unpaired student's t-test). G. Beta cell mass comparison between 11-week-old male PbkKI/KI and  PbkWT/WT mice  (n=4 for each group). **P = 0.0015 (two-tailed unpaired student's t-test). "
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "K, L. Representative images of double  immuno-staining  for insulin (green) and  BrdU  (red) of pancreas from PbkWT/WT (K) or PbkKI/KI (L) mice with 5 weeks HFD  feeding. Islet area was circled with white dashed line. Nuclei  were labeled by DAPI (blue). Scale bar: 50 μm."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "E, F. JunD KD in  INS-1 cells using shRNA  upregulated Pbk protein levels (E) and mRNA levels (F).  qPCR data was from three independent experimets (n=3).***P = 0.0001 (shRNA-#1), ***P = 0.0007 (shRNA-#2) (two-tailed unpaired student's t-test). G, H. JunD  KD in PIME cells using shRNA increased  Pbk protein levels (G) and mRNA levels (H). qPCR data was from three independent experimets (n=3).*P = 0.0236 (shRNA-#1), *P = 0.0175 (shRNA-#2) (two-tailed unpaired student's t-test). "
NCBI gene IDs:
"JunD: 16478
Pbk: 52033"
Figure legend: "N. JunD overexpression in PIME cells suppressed Pbk protein levels (M, Lane 2 vs Lane 1), but did not affect Pbk expression in menin-null PIME cells (M, Lane 4 vs Lane 3)."
NCBI gene IDs:
"JunD: 16478
menin: 17283"
Figure legend: "J, K. The effect of T5224 on the binding of JunD,  menin, HDAC3, and acetylated histone3 at the Pbk  locus in PIME  cells (J) or  INS-1 cells (K). Three independent experimets (n=3). *P = 0.0490 (J, JunD), *P = 0.0479 (J, Menin), *P = 0.0154 (J, HDAC3), **P = 0.0029 (J, Ac-H3), *P = 0.0104 (K, JunD), **P = 0.0024 (K, Menin), ***P = 0.0002 (K, HDAC3), ***P = 0.0001 (K, Ac-H3), (two-tailed unpaired student's t-test). ns, not statistically significant difference."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "F.  Mouse primary  islets treated with MI-503 for 3 days were subjected ChIP assay to detect the  binding of menin, HDAC3, JunD, as well as histone acetylation  level at the  Pbk locus. Three independent experimets (n=3). *P = 0.0218 (Menin), *P = 0.0145 (HDAC3), *P = 0.0184 (Ac-H3) (two-tailed unpaired student's t-test). ns, not statistically significant difference."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "G. MI-503 increases  proliferation  of  menin-expressing PIME cells but not menin-null PIME cells. Three independent experimets (n=3). ***P &lt; 0.0001 (Two-way ANOVA)."
NCBI gene IDs:
"menin: 17283"
Figure legend: "K-M.  5 days of MI-503 treatment impacts the expression of Pbk at protein level (K) and mRNA level (L), as well as ki67 mRNA levels (M) in human primary islets  (donor number is four). qPCR data was from three independent experimets (n=3). **P = 0.0036 (L, 10 nM), **P = 0.0024 (L, 50 nM), **P = 0.0073 (L, 100 nM), *P = 0.0237 (M, 50 nM) (two-tailed unpaired student's t-test). ns, not statistically significant difference. "
NCBI gene IDs:
"ki67: 4288"
Figure legend: "E-L. Representative  images  of insulin (green) and Ki67 (red) double staining on mouse islets with 8 wks MI  treatment (E-H), and insulin  (green) or Pbk  (red)  double staining  on mouse islets (I-L).  Nuclei were labeled by  DAPI (blue). Islet area was circled by white dashed line.  Ki67 and Pbk staining are denoted by white arrows. Scale bars: 50 μm."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "O, P. GTT data for MI-463 or vehicle treated PbkKI/KI or PbkWT/WT mice (n=3 for each group) on 8 weeks HFD  (O) (*P = 0.0133 (Two way ANOVA), ns, not statistically significant difference.) and AUC of GTT data (P) (*P = 0.0442 (two-tailed unpaired student's t-test), ns, not statistically significant difference.)."
NCBI gene IDs:
"Pbk: 52033"
Figure legend: "Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones or control cells (Ctrl).Gene expression of Pdgfb,Ang1,Ang2,Tgfb1, and Sema3a was quantified by qRT-PCR and expressed as fold-change versus control muscles. Data represent individual values, with mean ± SEM (n = 3 or 4, as indicated); *P &lt; 0.05 and **P &lt; 0.01 by one-way ANOVA with Bonferroni multiple comparisons test, after data normalization by logarithmic transformation. TGF-β1: V Low versus V Med P = 0.0047; V Low versus V High P = 0.0379; Sema3A: V Low versus V High P = 0.0042; V Med versus V High P = 0.0179."
NCBI gene IDs:
"Ang1: 11727
Ang2: 11731
Pdgfb: 18591
Sema3a: 20346
Sema3A: 20346
TGF-β1: 21803
Tgfb1: 21803"
Figure legend: "Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones or control cells (Ctrl).In situ hybridization for Sema3A (green) and CD31 (red) mRNA, with nuclei staining by DAPI, on frozen muscle sections. The three lower panels represent a higher-magnification of the area in the white square in the top panel, as a merged image and as individual channels: arrows indicate cells expressing both transcripts (yellow), only Sema3A (green), or only CD31 (red). Scale bar = 30 μm.Quantification of the percentage of endothelial cells (CD31+) that express Sema3A (left group) and of Sema3A-expressing cells that are endothelial (CD31+, right group). Data represent the mean ± SEM of 12 individual fields of view from three independent muscles (15-94 nuclei/image, 642 total nuclei). No statistics was applied, as data represent complementary sets."
NCBI gene IDs:
"CD31: 18613
Sema3A: 20346"
Figure legend: "Relative gene expression was quantified in the ex vivo FACS-purified endothelial cells (CD31+) and NEM (CD11b+) and expressed as fold-change versus the V High group. Data represent individual values, with mean ± SEM (n = 3-7, as indicated); **P &lt; 0.01 by one-way ANOVA with Bonferroni multiple comparisons test, after data normalization by logarithmic transformation; Sema3A: V Low versus V High P = 0.0157."
NCBI gene IDs:
"Sema3A: 20346"
Figure legend: "Mice, implanted with control cells (Ctrl) or myoblasts expressing low (V Low) or medium (V Med) VEGF levels, were treated with the anti-NRP1A antibody blocking Sema3A/NRP1 binding (AbαNRP1A) or with control IgG2a. Analyses were performed after 1 week.D Tgfb1 gene expression in total skeletal muscles after the different treatments, expressed as fold-change versus the Ctrl IgG2a group. Data represent individual values, with mean ± SEM (n = 3 or 4, as indicated); *P &lt; 0.05 and **P &lt; 0.01 by one-way ANOVA with Bonferroni multiple comparisons test, after data normalization by logarithmic transformation; V Low IgG2a versus Ctrl IgG2a P = 0.0083; V Low IgG2a versus V Low AbαNRP1A P = 0.0315."
NCBI gene IDs:
"Tgfb1: 21803"
Figure legend: "Relative gene expression of Id1, specifically induced by activated SMAD1/5, and Pai1, specifically induced by activated SMAD2/3, in total muscle tissues 1 week after implantation with V Low-, V Med- and V High-expressing myoblast clones or control cells (Ctrl), expressed as fold-change versus the Ctrl group. Data represent individual values, with mean ± SEM (n = 3 or 4, as indicated); **P &lt; 0.01 and ***P &lt; 0.001 by one-way ANOVA with Bonferroni multiple comparisons test, after data normalization by logarithmic transformation; V Low versus Ctrl P = 0.0002; V Low versus V Med P = 0.005; V Low versus V High P = 0.0016."
NCBI gene IDs:
"Id1: 15901
Pai1: 18787"