EMBO/SourceData huggingface dataset:
Figure legend: " D, PGE2 activates MEF2 via EP3 receptor. NRVMs were infected with the 3xMEF2-Luc reporter and serum-starved for 20 hours. The cells were stimulated with DMSO or 1 μM PGE2 for 24 hours in the presence or absence of different EP receptor antagonists: AH6809 (10 μM, EP1- and EP2-antagonist) or 798106 (200 nM, EP3-antagonist) or L161,982 (2 μM, EP4-antagonist) Data information: values are mean±s.e.m In (D), n=3, independent experiments, * represents significant interaction between the two treatments (P&lt;0.05, Two-Way ANOVA, AH6809, P=0.1092; L798106, P=0.0002; L161,982, P=0.5579). The exact n and P values can be also found in the Source Data excel file for Figure 1"
NCBI gene IDs:
"MEF2: 498607///81518///309957///499497"
Figure legend: " B-D (B, D) NRVMs were infected with the 3xMEF2-Luciferase reporter and with recombinant adenoviruses encoding EGFP (AdGFP), RGS16, RGS2, p63ΔN, RGS-LSCII, or Gαt, as indicated. The cells were serum starved for 20 hours and stimulated with DMSO or 1 μM PGE2 for 24 hours. (C) NRVMs were infected with the 3xMEF2-Luciferase reporter and pretreated with the adenylyl cyclase inhibitor SQ22536 (100 μM) for 20 minutes or the Gi/o-protein inhibitor pertussis toxin (PTX, 100 ng/ml) for 20 hours in serum-starved conditions and treated with DMSO or 1 μM PGE2 for 24 hours. Data information: In (B-D), n=3, independent experiments, * represents significant interaction between the two treatments (P&lt;0.05, Two-Way ANOVA, RGS16, P&lt;0.0001; RGS2, P=0.1725; p63ΔN, P=0.0666; RGS-LSCII, P=0.0818, SQ22536, P=0,385; PTX, P&lt;0.0001; Gαt, P&lt;0,0001), values are mean±s.e.m. The exact n and P values can be also found in the Source Data excel file for Figure 2 "
NCBI gene IDs:
"Gαt: 
RGS-LSCII: 
RGS16: 360857
RGS2: 84583
p63: 246334"
Figure legend: " A,B NRVMs were infected with recombinant adenovirus encoding Flag-HDAC5 (A) or not infected (B) and serum starved for 20 hours. The cells were pretreated with the PKC-inhibitor bisindolylmaleimide I (2 μM) or the PKD-inhibitor BPKDi (3 μM) for 20 minutes and stimulated with 100 nM endothelin-1 (ET1) or 100 μM phenylephrine (PE) or 1 μM PGE2 for 4 hours, as indicated. HDAC phosphorylation was detected by immunoblotting with the anti-HDAC5 phospho-Ser-498 antibody, phosphorylation of PKD was assessed using anti-phospho-PKD antibodies (Ser-744/Ser-748 and Ser-916), representative images are shown from 3 independent experiments "
NCBI gene IDs:
"HDAC5: 84580"
Figure legend: " B NRVMs were infected with recombinant adenoviruses encoding EGFP (AdGFP) or RGS16 24 hours prior to stimulation, then Rac1 activity was measure Data information: In (A-C) representative images from at least 3 independent experiments are shown. In (B-D) values are mean±s.e.m., (B,C) n=4 3; *,P&lt;0.05 (Two-Way ANOVA, RGS16, P=0.0415 (B ), NSC effect on Rac1 activation, P=0.0412 (C); NSC effect on MEF2 induction, P&lt;0.0001. PGE2 effect was significant in Rac1 activation, P&lt;0.0001). The exact n and P values can be also found in the Source Data excel file for Figure 4"
NCBI gene IDs:
"RGS16: 360857"
Figure legend: " B After infection with Flag-HDAC5 and serum starvation, NRVMs were treated with 50 μM NSC23766 for 2 hours prior to stimulation. The cells were stimulated with 1 μM PGE2 for 1 hour. HDAC5 phosphorylation at Ser-498 was detected by immunoblot. The phosphorylation of HDAC5 at this site was not prevented by NSC Data information: In (A-C) representative images from 3 independent experiments are shown "
NCBI gene IDs:
"HDAC5: 84580"
Figure legend: " D MEF2 activity was determined in cells infected with the 3xMEF2-Luc reporter after serum starvation. Cells were stimulated with vehicle or 1 μM PGE2 for 24 hours after 1-hour of 30 μM IPA-3 pretreatment. IPA-3 inhibited the MEF2 activation by PGE2 Data information In (D) values are mean±s.e.m., n=3, biological replicates, *,P&lt;0.05 (two-way ANOVA, P&lt;0.0001). The exact n and P values can be also found in the Source Data excel file for Figure 5"
NCBI gene IDs:
"MEF2: 498607///81518///309957///499497"
Figure legend: " Top: GFP-HDAC5 infected NRVMs were serum-starved for a day, pretreated for two hours with the indicated compounds (BPKDi, 3 µM, IPA-3, 30 µM) alone or in combination, then were stimulated for 4 hours. Representative images. Scale bar is 10 µm. Bottom : Statistical analysis of cellular localization of HDAC5. GFP-HDAC5 localization (nuclear or cytosolic) was assessed in >100 cells of each sample (n=3, technical replicates). *, P&lt;0,05 (one-way ANOVA with Bonferroni post-hoc test, vehicle vs. ET1 or vs. PGE2, P&lt;0.0001; PGE2 vs. PGE2+BPKDi, P=0.0004; PGE2 vs. PGE2+BPKDi, P=0.0114; PGE2 vs. PGE2+BPKDi+IPA-3, P&lt;0.0001). The exact n and P values can be also found in the Source Data excel file for Figure 6 "
NCBI gene IDs:
"HDAC5: 84580"
Figure legend: " MEF2-lacZ reporter mice (BALB/c-background, 6-12 weeks old) were treated with 7 mg/kg lipopolysaccharide from Escherichia coli (O111:B4) or saline intraperitoneally and were sacrificed after 24 hours A mRNA-levels of different inflammatory cytokines, as indicated. The graphs show relative mRNA-levels, fold increase compared to saline-treated controls, normalized to 18s-content Data information: values are mean±s.e.m. The exact n values are shown in the bottom of the bar graphs. *,P&lt;0.05, Welch's two-tailed unpaired t-test was used for statistical analysis, n.s.=not significant P values: IL6, P=0.0007 (A); TNFα, P&lt;0.0001 (A)"
NCBI gene IDs:
"lacZ: 
IL6: 16193
MEF2: 17876
18s: 19791
TNFα: 21926"
Figure legend: " MEF2-lacZ reporter mice (BALB/c-background, 6-12 weeks old) were treated with 7 mg/kg lipopolysaccharide from Escherichia coli (O111:B4) or saline intraperitoneally and were sacrificed after 24 hours B PGE2 was quantified after mechanical homogenization of deeply frozen hearts using nano-liquid chromatography tandem mass spectrometry Data information: values are mean±s.e.m. The exact n values are shown in the bottom of the bar graphs. *,P&lt;0.05, Welch's two-tailed unpaired t-test was used for statistical analysis, n.s.=not significant P values PGE2, P=0.0016 (B"
NCBI gene IDs:
"lacZ: 
MEF2: 17876"
Figure legend: " MEF2-lacZ reporter mice (BALB/c-background, 6-12 weeks old) were treated with 7 mg/kg lipopolysaccharide from Escherichia coli (O111:B4) or saline intraperitoneally and were sacrificed after 24 hours C Histologic and macroscopic stainings of β-galactosidase-activity in MEF2-lacZ reporter mice show MEF2-activation (blue cells and precipitates, respectively) in the myocardium upon LPS-treatment. Saline-treated littermates served as control. Scale bar of histological stainings is 100 µm. Quantification of whole-heart stainings is shown in the right panel (pixel intensity in blue channel normalized to total intensity) Data information: values are mean±s.e.m. The exact n values are shown in the bottom of the bar graphs. *,P&lt;0.05, Welch's two-tailed unpaired t-test was used for statistical analysis, n.s.=not significant. In (C) representative images of myocardial sections are shown from 3 independent experiments and representative whole hearts of 6 independent experiments. P values blue pixel intensity, P=0.0083 (C)"
NCBI gene IDs:
"lacZ: 
MEF2: 17876"
Figure legend: " MEF2-lacZ reporter mice (BALB/c-background, 6-12 weeks old) were treated with 7 mg/kg lipopolysaccharide from Escherichia coli (O111:B4) or saline intraperitoneally and were sacrificed after 24 hours D Induction of PKD, HDAC5 and PAK2 in myocardial inflammations. Immunoblots were performed on extracts of hearts of saline- and LPS-treated mice. Representative blots are shown in the left panel, the quantification is in the right panel Data information: values are mean±s.e.m The exact n values are shown in the bottom of the bar graphs. *,P&lt;0.05, Welch's two-tailed unpaired t-test was used for statistical analysis, n.s.=not significant P values ); phospho-PKD-Ser-744/Ser-748, P=0.0049 (D); phospho-PKD-Ser-916, P=0.2684 (D); phospho-HDAC5-Ser-498 (D), P=0.0019; phospho-HDAC5-Ser-259, P=0.0003 (D); phospho-PAK2-Ser-192/Ser-197, P=0.1962 (D); phospho-PAK2-Thr-402, P=0.0446 (D). The exact n and P values can be also found in the Source Data excel file for Figure 7"
NCBI gene IDs:
"lacZ: 
MEF2: 17876"
Figure legend: "(C) Relative mRNA expression from real-time PCR analysis of the GnRH transcript in GFP-positive cells, in comparison with GFP-negative cells. The GnRH transcript appears to be selectively expressed in GFP-positive cells. Mann-Whitney U test, n=4 mice from 2 litters."
NCBI gene IDs:
"GnRH: 14714"
Figure legend: "(D) Relative mRNA expression from real-time PCR analysis of Neuropilin-1 (Nrp1), Nrp2, Plexin A1 (PlxnA1), and PlxnA4 transcripts in FACS-isolated GFP-positive GnRH neurons from control (Nrp1loxP/loxP; grey) and mutant (Gnrh::cre; Nrp1loxP/loxP; red) P0 mice. Unpaired t-test, n=5 to 6 mice per group from 3 litters."
NCBI gene IDs:
"GFP: 
cre: 2777477
Gnrh: 14714
Neuropilin-1: 18186
Nrp1: 18186
Nrp2: 18187
Plexin A1: 18844
PlxnA1: 18844
PlxnA4: 243743"
Figure legend: "(E) Representative immunofluorescence images showing GnRH neurons migrating along Nrp1-immunoreactive vomeronasal/terminal nerve fibers (empty arrowheads) at the nose-brain junction in sagittal slices from E14.5 control and mutant embryos. GnRH neurons (green) themselves express Nrp1 (red, white arrows) in control Nrp1loxP/loxP mice whereas Nrp1 is undetectable in GnRH neurons from mutant Gnrh::cre; Nrp1loxP/loxP littermates (black arrows). Scale bar: 50μm."
NCBI gene IDs:
"cre: 2777477
Gnrh: 14714
Nrp1: 18186"
Figure legend: "(D) Representative image of cleared brains and immunolabeling for GnRH at the level of the olfactory bulbs, in a control (left panel) and a mutant (right panel) postnatal brain. Knock-out animals show an accumulation of GnRH neurons in the accessory olfactory bulb (circle formed by the GnRH labelling, right panel). AOB, accessory olfactory bulb. Scale bar: 300μm."
NCBI gene IDs:
"GnRH: 14714"
Figure legend: "Wild-type and LGP2-KO HEK293  cells in 24-well plates were transfected with 200 ng/ml of short poly I:C IFN-β  and IP-10  mRNA expression was determined by RT-qPCR. The data represent the mean ± SD (n = 3, t-test, *p &lt; 0.05). LGP2  KO2 and KO3 are independently isolated LGP2  KO clones. Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"IP-10: 3627
LGP2: 79132
IFN-β: 3456"
Figure legend: "Wild-type and LGP2-KO HEK293  cells in 24-well plates were infected with SeV. IFN-β and IP-10 mRNA expression was determined by RT-qPCR. The data represent the mean ± SD (n = 3, t-test, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"IP-10: 3627
LGP2: 79132
IFN-β: 3456"
Figure legend: "(F) HEK293   cells in 24-well plates were transfected with LGP2    (0.1, 0.3, or 0.6 μg/well) and RIG-I (0.1 μg/well) expression vectors as indicated. 24 h after transfection, cells were stimulated with 200 ng of short poly I:C  for 4 hr. Total RNAs were extracted, and the expression of  IFN-β mRNA was determined by RT-qPCR   and normalized to GAPDH   (n = 3, t-test, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
GAPDH: 2597
IFN-β: 3456
RIG-I: 23586"
Figure legend: "(G) HEK293FT  cells were transfected with FLAG-tagged LGP2  and HA-tagged ubiquitin    expression vectors. Whole cell extracts (WCE) were prepared 24 h after transfection. Immunoprecipitation  was performed with anti-FLAG  antibody (IP: FLAG) and the proteins were detected by western blot analysis with the indicated antibodies."
NCBI gene IDs:
"FLAG: 
HA: 
ubiquitin: 
LGP2: 79132"
Figure legend: "(H) FLAG-tagged LGP2  was transfected into HEK293  cells. Cells were then infected with SeV  at MOI = 10 for 24 h. Immunoprecipitation  was performed with anti-FLAG  antibody, and the proteins were detected with the indicated antibodies."
NCBI gene IDs:
"FLAG: 
LGP2: 79132"
Figure legend: "A) HEK293  cells were transfected with FLAG-RIG-I  or FLAG-LGP2  expressing vector. Cells were then infected with SeV, and whole cell extracts were collected at indicated time points. Immunoprecipitation  was performed with anti-FLAG  antibody. The proteins were subjected to SDS-PAGE and detected by western blotting."
NCBI gene IDs:
"FLAG: 
LGP2: 79132
RIG-I: 23586"
Figure legend: "B) A549  cells transfected with HA-tagged ubiquitin  were stimulated with 200 ng/ml of short poly I:C for 6 h. PLA  was performed with anti-HA, anti-RIG-I, and anti-LGP2  antibodies. The number of PLA  signals were counted (t-test, *p &lt; 0.05, n = 20). Each dot represents the number of PLA  signals of each cell. Data information: Scale bars represent 10 μm. All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"HA: "
Figure legend: "C) HEK293  cells were transfected with empty and LGP2  expression vectors for 24 hr. PLA  was performed with anti-LGP2  and anti-K63-Ub  antibodies. The number of PLA  signals were counted (t-test, *p &lt; 0.05, n = 33). Each dot represents the number of PLA  signals of each cell. Data information: Scale bars represent 10 μm. All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132"
Figure legend: "A) HEK293FT  cells were transfected with FLAG-tagged LGP2  and HA-tagged Riplet  expression vectors. 24 h after transfection, cells were lysed, and WCE were prepared. Immunoprecipitation  was performed with anti-FLAG  antibody, and the proteins were detected by  western blotting  with the indicated antibodies."
NCBI gene IDs:
"FLAG: 
HA: 
LGP2: 79132
Riplet: 84282"
Figure legend: "B) HEK293FT  cells were transfected with FLAG-tagged LGP2  expression vector. 24 h after transfection, immunoprecipitation  was performed with anti-FLAG  antibody and control IgG. Immunoprecipitates  were subjected to  SDS-PAGE, and the proteins were detected by western blotting  with indicated antibodies."
NCBI gene IDs:
"FLAG: 
LGP2: 79132"
Figure legend: "C) HEK293FT  cells were transfected with FLAG-tagged LGP2, Riplet, and HA-tagged ubiquitin  expression vectors, and immunoprecipitation  was performed with anti-FLAG  antibody."
NCBI gene IDs:
"FLAG: 
HA: 
LGP2: 79132
Riplet: 84282"
Figure legend: "D) HEK293FT  cells were transfected with FLAG-tagged LGP2, Riplet, and myc-tagged K63-only-Ub  expression vectors. 24 h after transfection, immunoprecipitation  was performed with anti-FLAG  antibody."
NCBI gene IDs:
"FLAG: 
myc: 
LGP2: 79132
Riplet: 84282"
Figure legend: "E) WT and Riplet  KO HEK293  cells were transfected with FLAG-tagged LGP2  and myc-tagged K63-only-Ub  expression vectors, and immunoprecipitation  was performed with anti-FLAG  antibody."
NCBI gene IDs:
"FLAG: 
myc: 
LGP2: 79132
Riplet: 84282"
Figure legend: "(F, G) WT and Riplet  KO HEK293  cells were transfected with 200 ng/ml of short poly I:C for 6 h, and PLA  was performed with anti-LGP2  and anti-K63-Ub  antibodies. The number of PLA  signals were counted (t-test, *p &lt; 0.05, n = 100). Each dot represents the number of PLA  signals in each cell. Scale bars represent 10 μm. Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"Riplet: 84282"
Figure legend: "(A) Liquid chromatography-tandem mass spectrometry (LC-MS/MS) of LGP2  in Riplet-expressing cells. LC-MS/MS data was quantified using LFQ method. The chromatographic profiles for each ubiquitination  site are shown in the right panels. Schematic representation of ubiquitination  sites of RIG-I  and LGP2  are shown in the upper panel."
NCBI gene IDs:
"Riplet: 84282"
Figure legend: "(B) FLAG-tagged LGP2  fragments and HA-tagged Riplet  expression vectors were transfected into HEK293FT  cells. 24 h after transfection WCE were prepared. immunoprecipitation  was performed with anti-FLAG  antibody. Immunoprecipitates  were subjected to  SDS-PAGE, and the proteins were detected by western blotting with indicated antibodies. The data is a representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
HA: 
LGP2: 79132
Riplet: 84282"
Figure legend: "(C) FLAG-tagged LGP2  and FLAG-tagged LGP2-4KR expression vectors were transfected into HEK293FT  cells. 24 h after transfection, WCE were prepared. Immunoprecipitation  was performed with anti-FLAG  antibody. The data is a representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
LGP2: 79132"
Figure legend: "(A) RIG-I, WT LGP2, and KR mutants of LGP2  were transfected into HEK293  cells together with p125luc reporter plasmids.  Luciferase activities were determined 24 h after transfection. The data represent the mean ± SD (n = 3, t-test, *p &lt; 0.05). WCEs were subjected to  SDS-PAGE, and the proteins were detected by  western blotting (lower panels). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"luc: 
LGP2: 79132
RIG-I: 23586"
Figure legend: "(B) HE293  cells stably expressing GFP  (control), LGP2, or LGP2-4KR were transfected with p125luc reporter plasmids and then stimulated with indicated amounts of short poly I:C. WCEs were prepared 24 h after transfection and stimulation, and luciferase activity  was determined. The proteins were subjected to  SDS-PAGE and detected by western blotting with the indicated Abs. The data represent the mean ± SD (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"GFP: 
luc: 
LGP2: 79132"
Figure legend: "HEK293  cells stably expressing GFP, LGP2, or LGP2-4KR were transfected with 200 ng/ml of short poly I:C  (C-E), Total RNA was isolated at the indicated time points. The expression of IFN-β, IP-10, and Ccl5  mRNA was determined by RT-qPCR  and normalized to GAPDH. The data represent the mean ± SD (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"GFP: 
Ccl5: 6352
IP-10: 3627
LGP2: 79132
GAPDH: 2597
IFN-β: 3456"
Figure legend: "HEK293  cells stably expressing LGP2, or LGP2-4KR were infected with SeV  at MOI = 5 (F-H), Total RNA was isolated at the indicated  time points. The expression of IFN-β, IP-10, and Ccl5  mRNA was determined by RT-qPCR  and normalized to GAPDH. The data represent the mean ± SD (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"Ccl5: 6352
IP-10: 3627
LGP2: 79132
GAPDH: 2597
IFN-β: 3456"
Figure legend: "HEK293  cells stably expressing LGP2, or LGP2-4KR were infected with influenza A virus (Flu) at MOI =10 (I, J). Total RNA was isolated at the indicated  time points. The expression of IFN-β, IP-10 mRNA was determined by RT-qPCR and normalized to GAPDH. The data represent the mean ± SD (n = 3, two-way ANOVA, *p &lt; 0.05 Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"IP-10: 3627
LGP2: 79132
GAPDH: 2597
IFN-β: 3456"
Figure legend: "(K, L) HEK293  cell clones stably expressing LGP2  or LGP2-4KR at lower levels were transfected with short poly I:C, and the expression of mRNAs were determined by RT-qPCR. The data represent the mean ± SD (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132"
Figure legend: "A) Microarray  analysis was performed as described in materials and methods. A heatmap of the microarray  data of LGP2  and LGP2-4KR stably expressing cells stimulated with short poly I:C  for 12 h."
NCBI gene IDs:
"LGP2: 79132"
Figure legend: "B, LGP2  and LGP2-4KR stably expressing HEK293  cells were stimulated with 200 ng/ml of short  poly I:C, and the expression of the genes at the indicated  time points were determined by  RT-qPCR and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
GAPDH: 2597"
Figure legend: "C) LGP2  and LGP2-4KR stably expressing HEK293  cells were stimulated with 200 ng/ml of short poly I:C, and the expression of the genes at the indicated time points were determined by RT-qPCR  and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
GAPDH: 2597"
Figure legend: "LGP2  and LGP2-4KR stably expressing A549  cells were infected with influenza A virus (Flu) at MOI = 10, and the expression of the genes at the indicated  time points were determined by RT-qPCR and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
GAPDH: 2597"
Figure legend: "LGP2  and LGP2-4KR stably expressing A549  cells were infected with  influenza A virus  (Flu) at MOI = 10, and the expression of the genes at the indicated  time points were determined by  RT-qPCR and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
GAPDH: 2597"
Figure legend: "J, K) LGP2  and LGP2-4KR expressing vectors were transected into HeLa  cells with Riplet  expressing vector. 18 hr after transfection, cells were fixed, and then the PLA  signals of LGP2  and endogenous MAVS  were detected. Peroxisome  was visualized using CellLight Peroxisome-GFP  reagent. Colocalization  of GFP  (peroxisome) and the PLA  signals (red) were observed by confocal microscopy. Scale bars represent 10 μm (J). The colocalization  area was normalized to the area of the PLA  signals (n = 25, t-test, *p &lt; 0.05) (K). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
Riplet: 84282"