EMBO/SourceData huggingface dataset:
Figure legend: "D. Time-lapse of confocal microscope pictures of root meristematic cells tagged with GFP-GIP1 in Col-0 (top panel) and cycb1;1 cycb1;2 (bottom panel). GIP1 localizes at the nuclear polar caps, followed by co-localization with microtubules at the spindle and phragmoplast arrays. In cycb1;1 cycb1;2 double mutants, GIP1 exhibited an abnormal localization, being found at the spindle (magenta arrowheads) and phragmoplast (white arrowheads) midzones, which are normally devoid of the protein. Scale bars: 5 μm."
NCBI gene IDs:
"cycb1;2: 818444
cycb1;1: 829904"
Figure legend: "(A) BMDMs  were treated with E. coli  (MOI=100) for 30 minutes and further cultured for various length of time  as indicated. The dynamic of ENT3  expression was determined by measuring Slc29a3  transcripts via RT-qPCR. (B) Phagocytosis inhibitor, Cytochalasin D (CytoD), pretreated BMDMs  were incubated with E. coli (MOI=100) and subjected for Slc29a3  expression analysis via RT-qPCR  6 hours post-infection. Data information: The expression level was calculated relative to Rpl19  and normalized to untreated group as 1. The results shown are combined from three biological replicates (n=3), unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Rpl19: 19921
Slc29a3: 71279
ENT3: 71279"
Figure legend: "(D) Different bacterial components were applied as stimulants to BMDMs. Slc29a3  expression was analyzed after 30 minutes of stimulation and a total of 6 hours incubation. (E) BMDMs  were treated with different doses of LPS  for 30 minutes, harvested 6 hours post-stimulation, and subjected for RT-qPCR  analysis. Data information: The expression level was calculated relative to Rpl19  and normalized to untreated group as 1. The results shown are combined from three biological replicates (n=3), unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Rpl19: 19921
Slc29a3: 71279"
Figure legend: "(C) WT or (D) MyD88-/- BMDMs  were stimulated with 10 ng/ml LPS  or live  E. coli  (MOI=100) in the presence of 10 µM TBK  inhibitor, MRT 67307, and analyzed for Slc29a3 expression. Data information: The results shown are combined from three biological replicates (n=3), unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"MyD88: 17874
Slc29a3: 71279"
Figure legend: "(E) The 10 ng/ml LPS-treated BMDMs  were examined for Ifnb1 expression  after 30 minutes of stimulation and a total of 6 hours incubation. Data information: The results shown are combined from three biological replicates (n=3), unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Ifnb1: 15977"
Figure legend: "(F) BMDMs  were treated with 100 U/ml of IFN-β  for various length of time  and subjected for Slc29a3 expression. Data information: The results shown are combined from three biological replicates (n=3), unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Slc29a3: 71279"
Figure legend: "(B-E) BMDMs  from WT, IFNAR1-/- (B), or STAT1-/- (C) mice  were treated with 10 ng/ml LPS, live E. coli (MOI=100), or 100 U/ml IFN-β, harvested and examined for Slc29a3  induction. BMDMs  from WT, IFNAR1-/- (D) or STAT1-/- (E) mice  were treated with 100 U/ml  IFN-β, 50 µg/ml poly(I:C), or EMCV  (MOI=10), harvested and examined for Slc29a3  induction. Data information: The expression  level was calculated relative to Rpl19  and normalized to untreated group as 1. The results shown in B to E are from three biological replicates (n=3) Unpaired two-tailed Student's t-test was used for statistical analysis, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"IFNAR1: 15975
Rpl19: 19921
Slc29a3: 71279
STAT1: 20846"
Figure legend: "(F-H) The expression  dynamic of Slc29a3  was profiled in BMDMs  treated with two different doses of IFN-α (F),  IFN-β (G), IFN-γ (H) for various length of time  as indicated. Data information: The expression  level was calculated relative to Rpl19  and normalized to untreated group as 1. The results shown in F to H from two biological replicates (n=2). Unpaired two-tailed Student's t-test was used for statistical analysis, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Rpl19: 19921
Slc29a3: 71279"
Figure legend: "(A) The induction fold change (treated/untreated) of Slc29a3  and selected ISG transcripts were curated from INTERFEROME database. Shown is data extracted from 250 IU/ml  IFN-β  treated BMDMs. Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Slc29a3: 71279"
Figure legend: "(A-C) (A) BMDMs  were infected with EMCV  (MOI=10) and harvested at indicated time points. (B) THP-1 derived macrophages  were challenged with EV71  (MOI=5) and collected at different time points. (C) BMDMs  were co-cultured with 50 µg/ml  poly (I:C)  with various length of time  and harvested. The mRNA expression  of Slc29a3  or SLC29A3  was measured by RT-qPCR. The expression level was calculated relative to Rpl19  or RPLP0  and normalized to untreated group at time  0 as 1. Results are combined from three biological replicates (n=3) Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"Rpl19: 19921
RPLP0: 6175
Slc29a3: 71279
SLC29A3: 55315"
Figure legend: "(D, E) ENT3fl/fl and Cx3cr1cre/+ENT3fl/fl mice  (n=12) were challenged with EMCV  (104 PFU, i.p.) and monitored for the survival (D) and disease severity (E) for two weeks. Survival  results were analyzed by log-rank (Mantel-Cox) test, *p &lt; 0.05. Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"cre: 2777477
Cx3cr1: 13051
ENT3: 71279"
Figure legend: "(F, G) ENT3fl/fl and  Cx3cr1cre/+ENT3fl/fl mice  were challenged with 107 PFU EMCV  and measured the viral titer in the brain  at post-infection  day 3 by plaque assay (n=6). Shown are representative  plaque assay  images (F) and the quantification (G). Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"cre: 2777477
Cx3cr1: 13051
ENT3: 71279"
Figure legend: "(A) WT and ENT3-/- BMDMs  were infected with EMCV  (MOI=10), and the culture supernatants were harvested 24 hours post-infection to measure the production of IFN-α,  IFN-β, and  IFN-γ by ELISA. Shown are combined results from three biological replicates (n=3). Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, ***p &lt; 0.001. n.s. no significance. The combined data are shown as mean ± SEM."
NCBI gene IDs:
"ENT3: 71279"
Figure legend: "(D, E) The mitochondrial  respiratory capacity (D) and glycolysis capacity (E) of WT and ENT3-/- BMDMs  treated with 1000 U/ml IFN-β for 6 hours were measured by  extracellular flux analysis. Shown are the representative results of OCR  and ECAR  kinetics from six biological replicates (n=6). Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, ***p &lt; 0.001. n.s. no significance. The combined data are shown as mean ± SEM."
NCBI gene IDs:
"ENT3: 71279"
Figure legend: "(G, H) The replication of EMCV  in WT (G) and ENT3-/- BMDMs (H) were evaluated in the presence of exogenous 1 or 10 µM of ribonucleosides  (A, U, C, G) 9 hours post-infection  (MOI=10). The expression of EMCV-2A2B   was determined by RT-qPCR. Shown are results from three to four biological replicates (n=3-4). Data information: In G, H) the EMCV-2A2B  expression level was calculated relative to Rpl19  and normalized to untreated group at time  0 as 1. Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, ***p &lt; 0.001. n.s. no significance. The combined data are shown as mean ± SEM."
NCBI gene IDs:
"2A: 
2B: 
Rpl19: 19921
ENT3: 71279"
Figure legend: "(A) Illustration of strategy to track the viral uncoating upon infection. (B-D) (B) WT and ENT3-/- BMDMs  were infected with Syto82-labeled EMCV  (MOI=1) and subjected to  live imagining 30, 60 and 90 minutes post-infection. The arrowheads indicated the Syto82+ virus puncta in the cell. Scale bars represent 10 µm. (C) Quantification of Syto82  signal in BMDMs  after 60 minutes was performed by enumerating Syto82+ cells (%) and (D) the mean fluorescent intensity (MFI) per Syto82+ cell (D). Results were generated from images of three biological replicates (n=3), each with 4 fields analyzed. Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"ENT3: 71279"
Figure legend: "(E) WT and ENT3-/- BMDMs  were infected with Syto82-labeled EMCV  (MOI=1) and co-stained  with 100 nM Lysotracker Green, subjected to live imagining 60 minutes post-infection. Data were the representative images of two biological replicates (n=2). Scale bars represent 5 µm. (F) WT and ENT3-/- BMDMs   were infected with EMCV  (MOI=10) for 2 hours and then enriched the endosomes  and lysosomes. EMCV  particles in endosome- or lysosome-enriched fractions were quantified by  plaque assay. Shown are combined results from three biological replicates (n=3). Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"ENT3: 71279"
Figure legend: "(G-K) (G) Calu-3  cells were infected with  SARS-CoV-2  original strain or delta  variant at MOI=0.1 for 48 hours, harvested, and examined for SLC29A3  induction. (H) ENT3  knockdown in Calu-3  cells was performed by lentiviral  shRNA system. The knockdown efficiency was confirmed by RT-qPCR. ENT3  knockdown Calu-3 cells were infected with SARS-CoV-2 delta  variant and subjected for the viral replication analysis  24 hours post-infection. The virus released out of cells was measured by plaque assay (I), and the viral titer in the cells was measured by RT-qPCR (J). (K) The replication of SARS-CoV-2 delta  variant in scramble and ENT3  knockdown  Calu-3 cells were evaluated in the presence of exogenous 1 or 10 µM of ribonucleosides  (A, U, C, G) 24 hours post-infection  (MOI=0.1). The SLC29A3  expression level was calculated relative to RPLP0  and normalized to control as 1. The SARS-CoV-2 E gene expression level was calculated relative to RPLP0  to normalize the cell number. Shown are combined results from three biological replicates (n=3). Data information: Unpaired two-tailed Student's t-test was used for statistical analysis, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s. no significance. Data are shown as mean ± SEM."
NCBI gene IDs:
"E gene: 43740570
RPLP0: 6175
SLC29A3: 55315
ENT3: 55315"
Figure legend: "B Top, diagram of ASOs tested below, mapped to their position of complementarity on ApoER2. Bottom, RT-PCR of RNA isolated from HeLa cells transfected with the indicated ASO at a final concentration of 80 nM. RNA spliced forms are labeled. Quantification of the percentage inclusion is shown below the image and calculated as: Inclusion (%)=[exon 19 included/(exon 19 included+exon 19 skipped)] x 100."
NCBI gene IDs:
"ApoER2: 7804"
Figure legend: "D Top, RT-PCR analysis of ApoER2 exon 19 splicing in HeLa cells depleted of SRSF1 using an SRSF1-specific siRNA. Control is a non-specific siRNA. Bottom, Immunoblot analysis of SRSF1 from HeLa lysates depleted of SRSF1. β-actin is included as a control.E Quantification of exon 19 splicing in (D). Error bars show s.e.m. P-value was determined using Student's two-tailed t-test."
NCBI gene IDs:
"ApoER2: 7804
SRSF1: 6426"
Figure legend: "F Top, RT-PCR analysis of exon 19 splicing in a primary mouse kidney cell line depleted of SRSF1 by RNAi. Control is a non-specific siRNA. Bottom, Immunoblot analysis of SRSF1 from HeLa lysates depleted of SRSF1. β-actin is included as a control.G Quantification of exon 19 splicing in E. Error bars show s.e.m. P-value was calculated using Student's two-tailed t-test."
NCBI gene IDs:
"SRSF1: 110809
SRSF1: 6426"
Figure legend: "A Top, ASOs mapped onto the mouse ApoER2 exon 19 gene region. Bottom, RT-PCR of RNA isolated from mouse cells transfected with different ASOs. Quantification of percent exon 19 inclusion is shown below the gel image."
NCBI gene IDs:
"ApoER2: 16975"
Figure legend: "D RT-PCR analysis of RNA isolated from the hippocampus of mice treated as adults by ICV injection of indicated ASOs. C refers to a non-specific control ASO.E Quantitation of ApoER2 exon 19 inclusion from D (mean s.e.m., one-way ANOVA with Bonferroni multiple comparison test."
NCBI gene IDs:
"ApoER2: 16975"
Figure legend: "A RT-PCR analysis of ApoER2 exon 19 splicing in RNA isolated from hippocampus of 4 month old AD and WT mice treated with ASO-21 or ASO-C at P1-2.B Quantitation of exon 19 splicing. (mean s.e.m.; Kruskal-Wallis test with Dunn's multiple comparison test."
NCBI gene IDs:
"ApoER2: 16975"
Figure legend: "C Immunoblot for exon 19 containing ApoER2 (top) and total ApoER2 (bottom). β-actin was used as loading control.D Quantification of ApoER2 protein isoforms shown in C (mean s.e.m., student's t-test)."
NCBI gene IDs:
"ApoER2: 16975"
Figure legend: "(b) Immuno-gold EM micrographs of HeLa cells transfected with GFP-PLD1 and subjected to 1 h of nutrient deprivation in the presence (St+B) or absence (St) of bafilomycin (50 nM). Insets denote high magnification of the selected area. Scale bar, 500 nm. Right panel: Histogram showing morphometric analysis of the relative distribution of GFP-PLD1-positive gold particles on the outer (arrows) and inner (arrowheads) membranes of the autophagosomal-like structures. Values denote means±s.e.m. (n=14). **P0.01."
NCBI gene IDs:
"PLD1: 5337"
Figure legend: "(a) Confocal analysis of HeLa cells subjected to nutrient deprivation for 2 h. Cells were transfected with HA-PLD1 and GFP-ATG14L (upper panels) or with HA-PLD1 alone (lower panels). Upper panels: HA-PLD1 (red), GFP-ATG14L (green), endogenous LC3 (blue). Lower panels: HA-PLD1 (green), endogenous ATG16L (red) and LC3 (blue). Insets show high magnification of the selected areas. The triple insets on the right indicate high magnification of the selected areas for two channels in various combinations. Scale bar, 5 μm."
NCBI gene IDs:
"ATG14L: 22863
PLD1: 5337"
Figure legend: "(c) EM analysis showing that GFP-PLD1 does not localize to the isolation membrane (arrows) in cells expressing the strawberry-ATG4-C74A mutant. Immuno-gold EM micrographs of HeLa cells transfected with GFP-PLD1 and pStrawberry-ATG4-C74A and subjected to 1 h of nutrient deprivation. Inset denotes high magnification of the selected area. Scale bar, 250 nm."
NCBI gene IDs:
"ATG4: 23192
PLD1: 5337"
Figure legend: "(b) Western blot analysis of HA-PLD1 and endogenous LC3 levels in post-nuclear supernatant (PNS, upper panel), as well as in cytosolic/soluble (S) and particulate (P) fractions prepared from PNS after high-speed centrifugation (middle panel). GAPDH was used as loading control in PNS fractions. HeLa cells were transfected with expression vectors encoding HA-PLD1 for 24 h and subjected to 2 h of nutrient deprivation in the presence or absence of the PI3K inhibitor wortmannin (100 nM). Lower panel: quantitative analysis of HA-PLD1 immunoreactivity expressed as P/S ratio. Conditions were as follows: normal medium (Nm); normal medium with wortmannin (Nm+W); starvation condition (St); starvation condition+wortmannin (St+W). Values denote means±s.e.m. (n=3)."
NCBI gene IDs:
"PLD1: 5337"
Figure legend: "(a) Western blot analysis showing the effects of siRNA-mediated Vps34 (siVps34) downregulation on the expression levels of HA-PLD1 and Vps34 compared with control cells (siCtrl). Cells were incubated in normal medium (Nm) or subjected to 2 h of nutrient deprivation in the presence of 50 nM bafilomycin (St+B). HeLa cells were transfected for 48 h with siRNA directed to Vps34 (siVps34) or a control sequence (siCtrl) and then transfected for 24 h with a plasmid encoding HA-PLD1."
NCBI gene IDs:
"Vps34: 5289
PLD1: 5337"
Figure legend: "(b) Confocal imaging showing the effects of Vps34 (siVps34) downregulation on HA-PLD1 (green), endogenous LC3 (blue) and Lamp1 (red) on 2 h of nutrient deprivation compared with control treatment (siCtrl). Scale bar, 5 μm. (c) Confocal analysis showing the subcellular localization of HA-PLD1 mutated in its PX domain (F120A/R179Q), so as to abolish phosphoinositide binding (HA-PXmut). HeLa cells were transiently transfected with a plasmid encoding HA-PXmut and fixed after 2 h of nutrient deprivation. HA-PXmut (green), endogenous LC3 (blue) and Lamp1 (red). Scale bar, 10 μm. In b and c, insets show high magnification of the selected areas. The triple insets on the right indicate high magnification of the selected areas for two channels in various combinations."
NCBI gene IDs:
"Vps34: 5289
PLD1: 5337"
Figure legend: "(c) Immunostaining of endogenous LC3 in Pld1 WT and KO MEFs showing a reduced number and size of the LC3-positive compartment in KO MEFs. The DAPI staining is shown in blue. Cells were deprived of nutrients for 2 h in the absence (St, upper panels) or presence of bafilomycin (St+B, lower panels). Scale bar, 5 μm. (d) Quantification of the average size (top panel) and number of LC3-positive compartments per cell (bottom panel) in Pld1 WT and KO MEFs starved for 2 h. Values denote means±s.e.m. (n=6, number of cells=26-45 for all the conditions). **P0.01; *P0.05."
NCBI gene IDs:
"Pld1: 18805"
Figure legend: "(a) Immunofluorescent detection of endogenous LC3 (red) in liver sections from fed or starved Pld1 WT and KO mice. Sections were counterstained with DAPI. Scale bar, 5 μm. (b) Quantitative analysis of endogenous LC3 immunofluorescence in liver sections derived from fed or starved Pld1 WT and KO mice. Histograms show the average number, size and surface area of LC3-positive compartments. Values denote means±s.e.m. ((n=25-28), 4 mice per genotype). **P0.01."
NCBI gene IDs:
"Pld1: 18805"
Figure legend: "(c) Electron microscopic analysis showing a reduction in the size and number of autophagosome/amphisome-like structures (AP) in PLD1-deficient mice after food restriction. Representative electron micrographs from animals analysed in a showing APs (arrows) and autophagolysosomes/lysosomes (AL, arrowheads) in liver sections from starved WT and KO mice. Insets show high magnification of the selected areas. Scale bar, 1 μm. (d) Morphometric analysis of the number (per 100 μm2 cytoplasmic area) and relative surface area (% cytoplasmic area) of APs and ALs. Values denote means±s.e.m. (n=36 electron micrograms, from a total of 3,600 μm2 cytoplasmic area per genotype). **P0.01. (c) Morphometric analysis of the diameter of APs and ALs in the liver of PLD1-deficient mice after food restriction. Values denote means±s.e.m. (n=60). **P0.01."
NCBI gene IDs:
"PLD1: 5337"
Figure legend: "(D) FIBCD1 expression  in various human   visceral tissues and brain  regions (inset). Expression  is plotted relative to the tissue with lowest detectable expression  (trachea; inset, choroid plexus). n represents technical replicates (n = 2)."
NCBI gene IDs:
"FIBCD1: 84929"
Figure legend: "(A) Immunofluorescent  images of control and neuronal  (Nsyb) CG10359  (dFibcd1) RNAi-mediated knockdown D. melanogaster, 3rd instar larvae NMJ  (NMJ6/7) stained  with anti-horseradish peroxidase  antibodies. Empty control and RNAi-mediated knockdown of CG10359  (dFibcd1-i) lines #1, 2 and 3 shown. Scale bar = 20µm. Representative images of 3 independent experiments. Quantification of (A), control and CG10359  knockdown lines NMJ neuron bouton  number (B) n(empty) = 12; n(line #1) = 20; n(line #2) = 11; n(line #3) = 14. Data information: each data point represents an individual NMJ  Data is represented as mean and error bars represent SEM. p values were calculated using 2-way ANOVA (panels B * = p≤0.05; ** =p≤0.01; *** =p≤0.001; **** p≤0.0001."
NCBI gene IDs:
"CG10359: 38434
Fibcd1: 38434
syb: 36080"
Figure legend: "(E) Representative coronal section images of Golgi-Cox staining  of Fibcd1  WT and KO hippocampi  (left), Neurolucida tracing of  hippocampal CA1 pyramidal neurons (middle) and apical dendrites with spines  (right). Scale bars as indicated."
NCBI gene IDs:
"Fibcd1: 98970"
Figure legend: "(B) Immunoblot  analysis (left) and quantification of signal intensity (right) of Fibcd1  WT (blue) versus Fibcd1  KO littermates (red) adult hippocampi  with antibodies against CS-0S, CS-4S, CS-6S and actin  as a loading control. Each lane represents an independent animal (n = 3). Protein marker sizes are indicated. Data information: For panel B, each data point represent hippocampal  protein isolates from an individual mouse, Data are shown as mean values +/- SEM. p values were calculated using paired Student's t-test (panels B * = p≤0.05; ** =p≤0.01; *** =p≤0.001; **** p≤0.0001."
NCBI gene IDs:
"Fibcd1: 98970"
Figure legend: "(F) Confocal  images depicting internalisation  of FITC-tagged CS-4S  by FIBCD1-overexpressing HEK293T  lines compared to untransduced cells and unstained cells. Left, representative images; right, quantification. Data is plotted as total puncta per condition (n = 5). Cells are further stained  with CellMask Orange (cellular membrane) and Hoechst  (nuclei). Scale bar = 50µm. Data information: for panels F, each data point represents an individual cell preparation. Data are shown as mean values +/- SEM. p values were calculated using 1-way ANOVA (panels F, * = p≤0.05; ** =p≤0.01; *** =p≤0.001; **** p≤0.0001."
NCBI gene IDs:
"FIBCD1: 84929"
Figure legend: "(A) Left, representative image of immunofluorescent staining  (MAP2, red; DAPI, blue) of primary hippocampal  cultures at 2 days in vitro (DIV), plated on +/- CSPG  coating with and without prior digestion with ChABC, as indicated. Right, quantification of DIV2 images, showing the number of protruding cells per field normalised to untreated condition. n(Fibcd1  WT) = 4; n(Fibcd1  KO) = 3. Scale bar = 250µm. (B) Left, representative images of DIV14 neurons, same conditions as in (A). Right, quantification of DIV14 images, representing the percentage of cells per field that are clumped. n(Fibcd1  WT) = 3; n(Fibcd1  KO) = 2. Scale bar = 250µm. Data information: For panels A and B each data point represents an individual preparations of primary cell culture. Data are represented as mean and error bars represent SEM. p values were calculated by 1-way ANOVA. * = p≤0.05; ** =p≤0.01; *** =p≤0.001."
NCBI gene IDs:
"Fibcd1: 98970"
Figure legend: "A αSMA mRNA levels in TGFβ-treated HFL1 fibroblasts and the effect of the IRE1α inhibitor 4μ8C. *P = 0,001."
NCBI gene IDs:
"αSMA: 59"
Figure legend: "B Collagen1α2 mRNA levels in TGFβ-treated HFL1 fibroblasts ± the IRE1α inhibitor 4μ8C. *P = 0,01."
NCBI gene IDs:
"Collagen1α2: 1278"
Figure legend: "C mRNA levels of ER-stress related genes in TGFβ-treated HFL1 fibroblasts. *P CHOP = 0,045, *P Bip = 0,023, *P spliced XBP-1 P = 0,001, compared to corresponding control."
NCBI gene IDs:
"CHOP: 1649
Bip: 3309
XBP-1: 7494"
Figure legend: "E Agarose gel showing the presence of spliced and unspliced XBP-1 mRNA in TGFβ-treated HFL1 fibroblasts ± the IRE1α inhibitor 4μ8C."
NCBI gene IDs:
"XBP-1: 7494"