EMBO/SourceData huggingface dataset:
Figure legend: "Free mitochondrial NAD+ levels measured using a ratiometric biosensor in HeLa cells co-expressing empty vector control (n = 8 biological replicates), wildtype FlagSLC25A51 (n = 8 biological replicates), and indicated mutants (n = 4 biological replicates). Measurements were taken at 24 hours post-transfection; the dashed line indicates the baseline defined by the empty vector control, and red denotes data equivalent to wildtype. Data are shown in box and whisker format, with hinges at 25th and 75th percentiles, whiskers represent min and max and the line is the median, ANOVA p &lt; 0.0001 with post-hoc Dunnett's test compared to empty vector control ***p &lt; 0.001. (bottom) Protein expression from HeLa cells transiently transfected with empty vector control, wildtype FlagSLC25A51 and the indicated variants using anti-Flag western blot; HSP60, loading control."
NCBI gene IDs:
"Flag: 
SLC25A51: 92014"
Figure legend: "Relative uptake of 32P-NAD+ after 1 hour by E. coli cells expressing wildtype SLC25A51 (amino acids 29-297) or the E132A mutant when competed by 100 μM (n = 3 biological replicates) and 250 μM (n = 4 biological replicates) unlabeled NAD+ (red) or NADH (black). Data is shown as floating bars with central line at mean and the box represents the min and max value, unpaired two-sided t-test *p &lt; 0.05."
NCBI gene IDs:
"SLC25A51: 92014"
Figure legend: "Quantitation of 32P-NAD+ uptake after 1 hour in E. coli cells expressing wildtype SLC25A51 (amino acids 29-297) and indicated mutants; wildtype activity (n = 19 biological replicates) and equivalent, red. Data are shown in box and whisker format, with hinges at 25th and 75th percentiles, whiskers represent min and max and the line is the median, n = 5 - 8 biological replicates, ANOVA p &lt;0.0001 with post-hoc Dunnett's test compared to wildtype ***p &lt; 0.001."
NCBI gene IDs:
"SLC25A51: 92014"
Figure legend: "Free mitochondrial NAD+ levels measured using a ratiometric biosensor in HeLa cells expressing empty vector control (n = 27 biological replicates), wildtype FlagSLC25A51 (n = 27 biological replicates), and indicated mutants (n = 4 - 7 biological replicates). Measurements were taken at 24 hours post-transfection; the dashed line indicates the baseline defined by the empty vector control, and red denotes data equivalent to wildtype. Data are shown in box and whisker format, with hinges at 25th and 75th percentiles, whiskers represent min and max and the line is the median, ANOVA p &lt; 0.0001, post-hoc Dunnett's test compared to empty vector control ***p &lt; 0.001. (bottom) Protein expression from HeLa cells transiently transfected with empty vector control, wildtype FlagSLC25A51 and the indicated variants detected by anti-Flag western blot; HSP60, loading control."
NCBI gene IDs:
"Flag: 
SLC25A51: 92014"
Figure legend: "Free mitochondrial NAD+ levels measured using a ratiometric biosensor in HEK293 SLC25A51 KO cells expressing empty vector control, wildtype FlagSLC25A51, and indicated mutants. Measurements were taken at 48 hours post-transfection; the dashed line indicates the baseline defined by the empty vector control, and red denotes data equivalent to wildtype. Data are shown in box and whisker format, with hinges at 25th and 75th percentiles, whiskers represent min and max and the line is the median, n = 4 - 6 biological replicates, ANOVA p &lt; 0.0001, post-hoc Dunnett's test compared to empty vector control **p &lt; 0.01, ***p &lt; 0.001. (bottom) Protein expression from HEK293 SLC25A51 KO cells transiently transfected with empty vector control, wildtype FlagSLC25A51 and the indicated variants detected with anti-Flag western blot; HSP60, loading control."
NCBI gene IDs:
"Flag: 
SLC25A51: 92014"
Figure legend: "Schistosomal- miRNA was extracted from either EVs that were isolated from schistosomal-growing medium or control pellets that were isolated from unused schistosomal-medium as described in the Materials and Methods (Schistosomal-EVs purification section). Equal aliquots were subjected to qRT-PCR with specific primers to the schistosomal-miR-10, Bantam and miR-125; the expression is presented as ∆Ct from background Ct that was set as 40 cycles."
NCBI gene IDs:
"miR-125: 
Bantam: RF00727
miR-10: RF00104"
Figure legend: "The trans-well system was performed as in Fig. 1. Schistosome-exposed Th cells or control Th cells were subjected to qRT-PCR with specific schistosomal-Taqman primers to (A) miR-10-5p, (B) Bantam, and (C) miR-125b."
NCBI gene IDs:
"miR-125b: 
Bantam: RF00727
miR-10-5p: RF00104"
Figure legend: "The trans-well system was performed as in Fig. 1. The Control wells contained only unused schistosomal-medium; Supernatant wells contained 0.1 μm of the filtered schistosomal-growing medium; Th wells contained sorted CD4+CD11c- from spleens; Th+APCs contained sorted CD4+CD11c- and CD4-CD11c+ in 5:1 ratio, respectively. In Data information: In (A-E) the expression is presented as arbitrary units calculated as 2∆∆CT of miRNA relative to snRNA U6 and in (F) mRNA relative to β2 microglobulin. The mean +/- SEM was calculated from at least 3 independent experiments (except in F of 2 independent experiments)."
NCBI gene IDs:
"U6: 
β2 microglobulin: "
Figure legend: "Th cells were isolated from the indicated lymph nodes and spleen. In the case of uninfected mice, the Th cells from different lymph nodes and the spleen were mixed. qRT-PCR was performed with specific Taqman primers to either schistosomal-miR-10 or -Bantam. The presence of the miRNAs is presented as arbitrary units calculated as 2∆∆CT of the miRNA divided by snRNA U6. The mean +/- SEM was calculated from 4 independent experiments."
NCBI gene IDs:
"U6: 
Bantam: RF00727
miR-10: RF00104"
Figure legend: "HEK-293 cells were transfected with a plasmid expressing schistosomal-miR-10 at the indicated concentration together with either psiCHECK-II vector, psiCHECK-WT-MAP3K7-3'UTR or psiCHECK-mut-MAP3K7. After 24h, the cells lysates were subjected to luciferase assay. The results are presented as the ratio of renilla/luciferase expression that was normalized relative to cells transfected only with psiCHECK-II. Values are expressed as the mean+/-SD of at least 3 independent experiments."
NCBI gene IDs:
"luciferase: 
MAP3K7: 6885
miR-10: RF00104"
Figure legend: "A representative experiment in which protein extracted from Jurkat-T cells stably over expressing stably control vector or miR-10 expressing vector were subjected to WB analysis with anti-MAP3K7 or anti-GAPDH antibodies. The graph presents densitometry analysis of 3 WBs analyses performed as in (C). The mean +/- SD was calculated from 4 independent experiments. Statistics were performed using t-test (*p&lt;0.05)."
NCBI gene IDs:
"miR-10: RF00104"
Figure legend: "Jurkat-T cells stably expressing either control vector or a vector containing schistosomal-miR-10 were co-transfected with a control Luciferase plasmid or NF-κB luciferase reporter vector. In all transfections, a plasmid expressing Renilla vector as an internal control was added. 48h post-transfection, cells were harvested and were analyzed by luciferase reporter assay. Values were normalized to the Renilla activity. Data are represented as mean +/-SEM from 5 independent experiments. Statistics were performed using t-test, **p&lt;0.01."
NCBI gene IDs:
"Luciferase: 
luciferase: 
miR-10: RF00104"
Figure legend: "(C) LacZ staining (blue) to detect gene expression of the knocked-in LacZ cassette from the Abca12 locus in islets (islet outlined, scale bar = 25μm)."
NCBI gene IDs:
"LacZ: 
Abca12: 74591"
Figure legend: "(D,E) ABCA12 immunostaining and the development of hyperkeratotic skin disease in E18.5 Abca12tm1a/tm1a mouse embryos (brackets indicate thickened epidermis, dashed line indicates epidermal basement membrane, scale bar = 50μm)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(H) Detection of recombination of the Abca12 locus by PCR, indicating different alleles (tm1c, wild type and tm1d)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(A-F) Oral and intraperitoneal glucose tolerance testing in Abca12tm1d mice at 6 (A,B), 8 (C, D) and 12 weeks (E,F) (mean±SEM, n=4-9 mice per group, * p=&lt;0.05, **p&lt;0.01 Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(H) Whole blood Hb1Ac levels in 16 week old Abca12tm1d mice (mean±SEM, n=4-9 mice per group, **p&lt;0.01 Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(I) Acute phase insulin secretion after intraperitoneal injection of glucose (mean±SEM, n=4-9 animals per group, white/grey/black = Abca12+/+, cre/+ and Abca12tm1d respectively, mice at 8 weeks of age, **p&lt;0.01 Students t-test)."
NCBI gene IDs:
"Abca12: 74591
cre: 2777477"
Figure legend: "(J) Silencing of ABCA12 in MIN6 cells as assessed by Western blot of ABCA12 immuno-precipitate (top panel) or confocal microscopy in cells transfected with Dy547-siRNAABCA12 (lower panel, scale bar = 10μm)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(K) Glucose stimulated insulin secretion (GSIS) from MIN6 cells after silencing of ABCA12 (mean±SEM, n=4 replicates from assays in quadruplicate, ***p&lt;0.0001, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(L) Cellular insulin content in MIN6 cells after silencing of ABCA12 (mean±SEM, n=4 replicates from assays in quadruplicate)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(D) Western blot of cell lysates of MIN6 cells after silencing of Abca12, probed with antibodies against ABCA1, ABCG1, SR-B1 and LXRβ."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(E) Total cholesterol (COH) or cholesteryl esters (CE) levels in isolated pancreatic islets of mice at 8 weeks of age with deletion of Abca12 in β-cells (mean±SEM n=9, 5 and 5 mice per genotype respectively)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(F) Ceramides levels in isolated pancreatic islets of mice at 8 weeks of age with deletion of Abca12 in β-cells (mean±SEM n=10, 4 and 5 mice per genotype respectively)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(G) Lipid levels in MIN6 cells in which ABCA12 had been depleted by siRNA treatment (mean±SEM, n=5 (biological replicates assayed in triplicate), FC = free cholesterol, CE = cholesterol ester, Cer = ceramide, SM = sphingomyelin, TG = triacylglycerol, PC = phosphatidylcholine, *p&lt;0.05, **p=&lt;0.01, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(H) Cholesterol efflux to lipid-free apoA-I (30 μg/ml, 2 h) from MIN6 cells transfected with siRNAscr or siRNAABCA12 and activated or not with TO901317 (4 μM)(mean±SEM, n=4, (biological replicates assayed in triplicate), *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(I) Cholesterol efflux to HDL (30 μg/ml, 2 h) from MIN6 cells transfected with siRNAscr or siRNAABCA12 and activated or not with TO901317 (4 μM); (mean±SEM, n=4 (biological replicates assayed in triplicate))."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(J) The effect of overexpression of ABCA1 on GSIS from MIN6 cells with ABCA12 deficiency (mean±SEM, n=4 (biological replicates assayed in triplicate), *p=&lt;0.05, Students t-test), inset shows a Western blot of cell lysates probed with anti-ABCA1 antibody in which the order of bands corresponds to the order of bars)."
NCBI gene IDs:
"ABCA1: 11303
ABCA12: 74591"
Figure legend: "(K) The effect of overexpression of ABCG1 on GSIS from MIN6 cells with ABCA12 deficiency (mean±SEM, n=4 (biological replicates assayed in triplicate), *p=&lt;0.05, Students t-test, inset shows a Western blot of cell lysate probed with anti-ABCG1 anitbodies in which the order of bands corresponds to the order of bars)."
NCBI gene IDs:
"ABCA12: 74591
ABCG1: 11307"
Figure legend: "(L) The effect of overexpression of ABCA1 and ABCG1 on GSIS from MIN6 cells with ABCA12 deficiency (mean±SEM, n=4 (biological replicates assayed in triplicate), *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA1: 11303
ABCA12: 74591
ABCG1: 11307"
Figure legend: "(M) The effect of activation of cells with TO901317 (4 μM) on GSIS from MIN6 cells with ABCA12 deficiency (mean±SEM, n=4 (biological replicates assayed in triplicate), *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(N) The effect of overexpression of LXRβ on GSIS from MIN6 cells with ABCA12 deficiency (mean±SEM, n=4 (biological replicates), *p=&lt;0.05, Students t-test, inset shows a Western blot of cell lysates probed with anti-LXRβ antibody in which the order of bands corresponds to the order of bars)."
NCBI gene IDs:
"ABCA12: 74591
LXRβ: 22260"
Figure legend: "(B) Scatter plot and heat map of differential gene expression between cre control and Abca12tm1d mice at 8 weeks of age. Legend indicates Log2 fold change values."
NCBI gene IDs:
"Abca12: 74591
cre: 2777477"
Figure legend: "(C) Circulating glucose levels after administration of intraperitoneal L-arginine in mice at 8 weeks of age, mean±SEM, n =3 mice per genotype, *p=&lt;0.05 of cre relative to tm1d, Students t-test)."
NCBI gene IDs:
"cre: 2777477"
Figure legend: "(D) Representative images of insulin granule fusion events (yellow circles) in mice of indicated genotypes in response to administration of 15mM glucose (scale bar = 10μm). Quantification of the proportion of responding cells and the density of granule fusion events are presented in graphs in the lower panel (mean±SEM, 8-10 islets/mouse, &gt;4 mice/genotype, **p=&lt;0.01, ***p=&lt;0.001 of cre relative to tm1d, Students t-test)."
NCBI gene IDs:
"cre: 2777477"
Figure legend: "(E) Representative images of insulin granule fusion events (yellow circles) in mice of indicated genotypes in response to administration of 20mM KCl (scale bar = 10μm). Quantification of the proportion of responding cells and the density of granule fusion events are presented in graphs in the right panel (mean±SEM, 8-10 islets/mouse, &gt;4 mice/genotype, **p=&lt;0.01 of cre relative to tm1d, Students t-test)."
NCBI gene IDs:
"cre: 2777477"
Figure legend: "(F) Assessment of calcium flux measured by Fluo4 in response to either Glucose or Potassium in isolated islets from 11 week old animals. Fluorescence responses were corrected for basal fill (F/F0). (mean±SEM, each data point represents the average of 10 cells/coverslip for 13-16 coverslips for each of 4 wild type and 5 Abca12tm1d mice)"
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(H) Insulin content of islets of Abca12tm1d mice (mean±SEM, n=3 mice of each genotype, normalised to total protein content)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(A-C) Transmission electron microscopy of β-cells from Abca12tm1d and control mice at 8 weeks of age. Panels A'-C' depict higher resolution images from the boxed regions in A-C (scale bar = 1μm)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(D) Quantitation of the area of 3000 granules from each genotype (mean±SEM, n=4, 3 and 3 animals respectively, *p=&lt;0.05 of cre relative to tm1d, Students t-test). (E) Relative frequency distribution of granule areas from (D). "
NCBI gene IDs:
"cre: 2777477"
Figure legend: "(G, H) Quantification of change in mean granule area in Abca12tm1d on chow diet and Abca12tm1d on high cholesterol diet islets assessed by transmission electron microscopy (n=3000 granules/genotype, mean±SEM, n=4, 3, 3 and 4 animals respectively, *p=&lt;0.05, Students t-test) as an average and as a relative frequency distribution."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(J) Glucose tolerance test results following intraperitoneal glucose challenge in Abca12tm1d and cre control mice fed normal chow or HCD (mean±SEM, n=3 mice per genotype, *p=&lt;0.05, for tm1d versus tm1d+HCD, Students t-test)."
NCBI gene IDs:
"Abca12: 74591
cre: 2777477"
Figure legend: "(K) Serum insulin in Abca12tm1d +/- HCD mice after i.p glucose challenge (mean±SEM, n=3 mice/genotype, **p=&lt;0.01, Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(C) Confocal images of rafts (using CTB-Alexa Fluor 647) in MIN6 cells transfected with Fluorescein-488-labelled siRNAScr or Dy547-labelled siRNAABCA12 (scale bar=10μm). (D) Quantitation by confocal microscopy of the effect of ABCA12-deficincy on CTB binding to MIN6 cells (mean±SEM, *p=&lt;0.05, Students t-test, n=4 biological replicates, 50-100 cells for each). (E) Quantitation by flow cytometry of the effect of ABCA12-deficiency on CTB binding to MIN6 cells (mean±SEM, n=3 biological replicates, *p=&lt;0.05, Students t-test). "
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(F) The effect 10 mM MβCD on GSIS in MIN6 cells depleted or not for ABCA12 (incubation for 5 or 10 minutes as indicated, mean±SEM, n=4 (biological replicates), *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(G) Western blot of the abundance of CDC42 in ABCA12 deficient MIN6 cells."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(H) The effect of ABCA12 deficiency on activation of CDC42 by bradykinin in MIN6 cells (BDK, 100 ng/ml, 4 min; *p=&lt;0.05, Students t-test, significance versus ABCA12-deficient BDK activated cells is indicated, n=4 biological replicates, mean±SEM)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(I,J) (I) Confocal images of F-actin in ABCA12-deficient MIN6 cells (scale bar = 20μm) and (J) quantification of abundance showing the effect of ABCA12-deficiency (mean±SEM, *p=&lt;0.05, Students t-test, n=4 biological replicates, 50-100 cells for each)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(K) F-actin levels assessed by FACS sorting of labelled MIN6 cells transfected with siRNAABCA12 (mean±SEM, n=3 biological replicates, *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591"
Figure legend: "(L,M) (L) Representative image of F-actin using LifeAct staining of purified islets isolated from Abca12tm1d versus control mice (scale bar = 10μm) and (M) quantitation of fluorescent signals from these samples (n=3 biological replicates, mean±SEM, ***p=&lt;0.001, ****p=&lt;0.0001 Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "O) Effects of overexpression of a constitutively active form of CDC42 on GSIS from MIN6 cells with ABCA12-deficiency (mean±SEM, n=4 biological replicates, *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"ABCA12: 74591
CDC42: 12540"
Figure legend: "(P) The effect of overexpression of consitutively active CDC42 on ABCA1 abundance in MIN6 cells with ABCA12-deficiency. Treatments are defined in the table below."
NCBI gene IDs:
"ABCA12: 74591
CDC42: 12540"
Figure legend: "(R) Effects of Abca12 knockdown and Jasplakinolide treatment on MIN6 cell in low and high glucose (mean±SEM, n=4 biological replicates, *p=&lt;0.05, **p=&lt;0.01, significance relative to untreated cells, Students t-test)"
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(A) Mass spectroscopy analysis of levels of cholesterol and cholesteryl esters in purified islets from Abca12tm1d mice versus control animals at 16 weeks of age (mean±SEM, n=10, 4 and 5 mice per genotype respectively; COH=cholesterol, CE=cholesteryl ester)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(B) Mass spectroscopy analysis of lipids families in purified islets from Abca12tm1d mice versus control animals at 16 weeks of age (mean±SEM, n=10, 4 and 5 mice per genotype respectively, **p=&lt;0.01 Students t-test); CE=cholesteryl ester, Cer=ceramide, DHC (Hex2Cer)=dihexosylceramide, MHC (HexCer)=monohexosylceramide, SM=sphingomyelin, GM3=GM3 ganglioside, dhCer=dihydroceramide, DG=diacylglyerol, TG=triacylglycerol)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(C) Mass spectroscopy analysis of triglyceride species in purified islets from Abca12tm1d mice versus control animals at 16 weeks of age (mean±SEM, n=10, 4 and 5 mice per genotype respectively, *p=&lt;0.05, **p=&lt;0.01, Students t-test)"
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(D) Immunohistochemical staining of levels IL-1β expression in sections of pancreas from Abca12tm1d mice versus control animals at 24 weeks of age (bar = 25μm, islets outlined)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "(E) Quantification of islet specific IL1β staining from sections of Abca12tm1d, Cre and wild type mice (mean±SEM, n=3, 3 and 4 mice of each genotype respectively; *p=&lt;0.05, Students t-test)."
NCBI gene IDs:
"Abca12: 74591
Cre: 2777477"
Figure legend: "Analysis of Abca12tm1d pancreata from mice at 24 weeks of age compared to control mice assessing β-cell mass (F) (I)(mean±SEM, n=3-9 mice per genotype, **p=&lt;0.01, ***p=&lt;0.001, Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "Analysis of Abca12tm1d pancreata from mice at 24 weeks of age compared to control mice assessing cleaved caspase 3 (G) (I)(mean±SEM, n=3-9 mice per genotype, **p=&lt;0.01, ***p=&lt;0.001, Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "Analysis of Abca12tm1d pancreata from mice at 24 weeks of age compared to control mice assessing glucagon (H) (I)(mean±SEM, n=3-9 mice per genotype, **p=&lt;0.01, ***p=&lt;0.001, Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "Analysis of Abca12tm1d pancreata from mice at 24 weeks of age compared to control mice assessing F480+ macrophage populations (I)(mean±SEM, n=3-9 mice per genotype, **p=&lt;0.01, ***p=&lt;0.001, Students t-test)."
NCBI gene IDs:
"Abca12: 74591"
Figure legend: "Heatmaps of AIB1-YAP co-activated, YAP activated, AIB1-YAP corepressed, and YAP repressed genes shown in panel (A). Red and blue indicate high and low levels, respectively. RNA-sequencing was performed in triplicate and statistics in Panel (A-B) were calculated by EdgeR with Benjamini-Hochberg multiple test correction in R."
NCBI gene IDs:
"AIB1: 8202
YAP: 10413"
Figure legend: "Select genes are regulated by YAP and AIB1, others are YAP regulated independently of AIB1. Endogenous gene expression changes assessed by RT-qPCR."
NCBI gene IDs:
"AIB1: 8202
YAP: 10413"
Figure legend: "AIB1 modulates TEAD transcriptional activity in a YAP dependent manner. HEK293T cells were transfected with CTGF-promoter reporter and expression vectors of indicated proteins."
NCBI gene IDs:
"CTGF: 1490
YAP: 10413"
Figure legend: "ANCO1 is recruited to YAP-TEAD by AIB1. Positive Proximity Ligation Assay (PLA) signal (white foci, falsely colored 594nm signal) shown between ANCO1-TEAD, ANCO1-YAP, AIB1-TEAD, and AIB1-YAP, in control cells are lost in AIB1 knockdown. Representative images of Proximity Ligation Assay (PLA) between indicated proteins in MCF10A shGFP (control) and shAIB1 cell lines using antibodies (species) as indicated; Scale bars shown indicate 25μm."
NCBI gene IDs:
"GFP: 
AIB1: 8202"
Figure legend: "YAP overexpression represses, AIB1 knockdown dysregulates, and ANCO1 knockdown amplifies genes at the 1q21.3 locus in MCF10A and MCFDCIS cells. Gene set enrichment analysis of RNA-seq data and cDNA array data generated from MCF10A cell lines as indicated."
NCBI gene IDs:
"ANCO1: 29123
AIB1: 8202
YAP: 10413"
Figure legend: "YAP represses 1q21.3 localized genes. Levels of endogenous S100 and SPPR genes in MCF10A cell lines following overexpression of YAP/S94A; levels were assessed by RT-qPCR."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "ANCO1 knockdown increases expression of S100 and SPPR genes in MCF10A and MCFDCIS cells; levels were assessed by RT-qPCR."
NCBI gene IDs:
"ANCO1: 29123"
Figure legend: "YAP driven suppression of 1q21 genes is reverted upon ANCO1 knockdown. Levels of endogenous 1q21.3 genes were assessed in YAP or S94A-expressing, control or shANCO1 MCF10A cells by RT-qPCR."
NCBI gene IDs:
"ANCO1: 29123
YAP: 10413"
Figure legend: "ANCO1 is recruited to superenhancers at 1q21.3 by AIB1 and is significantly reduced at 1q21.3 following AIB1 knockdown. ChIP performed with ANCO1 antibodies in control or shAIB1 cells. Levels of precipitated chromatin assessed by qPCR."
NCBI gene IDs:
"AIB1: 8202"
Figure legend: "YAP driven aberrant sphere formation of MCF10A cell lines is ablated by AIB1 knockdown, but further enhanced by ANCO1 knockdown. Indicated cell lines were plated on Matrigel and allowed to form mammospheres for 7 days. 10x and 40x magnifications shown, Scale bar = 200μm."
NCBI gene IDs:
"ANCO1: 29123
AIB1: 8202
YAP: 10413"
Figure legend: "Quantification of size (arbitrary units) of aberrant spheres per field displayed in panel (A), relative to control YAP S94A shGFP spheres. n= number of spheres."
NCBI gene IDs:
"GFP: 
YAP: 10413"