EMBO/SourceData huggingface dataset:
Figure legend: "D. Immunoprecipitation from MDA-MB-231 transiently transfected with control (pcDNA3) or HA‑RASSF1C plasmid. Pull‑down was performed using a SRC or same species IgG antibody and blotted  with indicated antibodies."
NCBI gene IDs:
"HA: 
RASSF1C: 11186"
Figure legend: "H. Quantification of rounded  versus elongated cells in single cell morphology assay in 3D‑collagen indicating the degree of mesenchymal-amoeboid transition of MDA-MB-231  cells when RASSF1C  or the described mutants are expressed. I. Representative confocal images show elongated or  rounded  cells per field of view of MDA‑MB-231 cells transfected with DsRed, DsRASSF1C, DsRASSF1C-R197W or DsRASSF1C-R199F, grown in 3D‑collagen and  stained with Phalloidin-568 (red) for  F‑actin and pMLCII/Alexa 633. Scale bars represent 10 μm. "
NCBI gene IDs:
"RASSF1C: 11186"
Figure legend: "A. Left: Cartoon showing the principle behind the Cre-carrying EVs and how recipient cells switch from DsRed+ to eGFP+ expression upon Cre-mediated excision of the  DsRed  locus. Centre: representative images, and right: quantification of  T47DDsRed cells that converted to  eGFP+ in the bottom wells of a transwell system in the presence of MDA-MB-231CFP;Cre;Control or MDA‑MB‑231CFP;Cre;HA-RASSF1C  seeded in the upper wells. Scale bars represent 100 μm. Data information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001."
NCBI gene IDs:
"CFP: 
HA: 
Cre: 2777477
RASSF1C: 11186"
Figure legend: "C. Western blot  analysis of EVs isolated from  MDA-MB-231 cells transfected with Control (pcDNA3) or FLAG-RASSF1C with indicated antibodies. Equal amounts of protein were loaded after normalization via microBCA assay."
NCBI gene IDs:
"FLAG: 
RASSF1C: 11186"
Figure legend: "D. Representative confocal images (upper row) and quantification of fluorescent intensity of  ALDH1 expression in MDA-MB-231  cells expressing RASSF1C (black bar in the graph) or after siRNA treatment against RASSF1  (grey bar). Lower row shows merged images with DAPI  (blue), ALDH1 (green) and RASSF1C (red). Scale bars represent 20 μm. Data Information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001."
NCBI gene IDs:
"RASSF1: 11186
RASSF1C: 11186"
Figure legend: "J. qRT‑PCR expression of NANOG,  OCT4, SOX2 in MCF7 cells 24 h after exposure to 50 ng/μl  EVs derived from MDA‑MB‑231 cells (treated as indicated). Data Information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. "
NCBI gene IDs:
"NANOG: 79923
OCT4: 5460
SOX2: 6657"
Figure legend: "B. Left: representative confocal images of mammary tumor formed by the co-injection of T47DDsRed reporter cells with either MDA-MB-231CFP;Cre;Control or MDA-MB-231CFP;Cre;HA-RASSF1C. Scale 200 μm. Right: averaged values for all mice for the percent of the  eGFP+ or CFP+ cells as part of the whole tumor. Data information: All data are from n=3 independent experiments. Data are analyzed by Student T-test and represented as mean ± SEM.* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001."
NCBI gene IDs:
"CFP: 
HA: 
Cre: 2777477
RASSF1C: 11186"
Figure legend: "B.  Kaplan-Meier  plots of overall  survival of 496 stage I and II breast cancer patients with different levels of promoter/CpG island  methylation of RASSF1A, previously reported to be associated with outcome in breast cancer patients. P values were obtained from a log-rank test."
NCBI gene IDs:
"RASSF1A: 11186"
Figure legend: "C. Left: heatmap depicting methylation  patterns across the RASSF1  gene. In light green, RASSF1-2γ  CpG island is depicted; in dark green, RASSF1-1α CpG island is depicted. Right: Kaplan‑Meyer survival curves  depicting interaction of RASSF1A    and RASSF1C methylation to predict the outcome of early stage breast cancer patients. P values were calculated from a log-rank test. Color legend: yellow, low RASSF1A   and low RASSF1C; green, low RASSF1A  and high RASSF1C; red, high RASSF1A  and low RASSF1C; grey, high RASSF1A and high RASSF1C."
NCBI gene IDs:
"RASSF1: 11186
RASSF1A: 11186
RASSF1C: 11186"
Figure legend: "Cytokines and innate immune mediators' profile in the brain of mice from different experimental groups: (F) IFN-β1, (G) ISG15, (H) Mx1, detected in one brain hemisphere of mice from different experimental groups. The expression of the genes of interest was normalized to the GAPDH housekeeping gene."
NCBI gene IDs:
"GAPDH: 14433
IFN-β1: 15977
ISG15: 100038882
Mx1: 17857"
Figure legend: "Cytokines and innate immune mediators' profile in the brain of mice from different experimental groups: , (I) IFN-γ, (J) CXCL10, (K) CCL5, (L) IL-6, (M) TNF-α and (N) IL-1β, detected in one brain hemisphere of mice from different experimental groups. The expression of the genes of interest was normalized to the GAPDH housekeeping gene."
NCBI gene IDs:
"CCL5: 20304
CXCL10: 15945
GAPDH: 14433
IFN-γ: 15978
IL-1β: 16176
IL-6: 16193
TNF-α: 21926"
Figure legend: "A. Representative FISH images showing RAB13 RNA distribution in MDA-MB-231 cells. Nuclei and cell outlines are shown in blue and green respectively. Arrows points to RAB13 RNA concentrated at protrusive regions. Boxed regions are magnified in the insets."
NCBI gene IDs:
"RAB13: 5872"
Figure legend: "B. Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Calibration bar shows intensity values. C. Ratios of peripheral/perinuclear intensity calculated from images as shown in A and B. Bars: mean ± s.e.m..Values within each bar represent number of cells observed in 3 independent experiments. "
NCBI gene IDs:
"RAB13: 5872"
Figure legend: "B. FISH images of mouse fibroblasts expressing the β-globin coding sequence followed by the indicated UTRs. β-globin RNA is shown in yellow. Nuclei and cell outlines are shown in blue. Arrows points to β-globin RNA concentrated at protrusive regions. ∆1 and ∆1+2 indicate deletions of the regions shown in (A). Scale bars: 10 μm. C. Distribution of β-globin RNA, or of Ddr2 RNA detected in the same cells, quantified by measuring a Peripheral Distribution Index (PDI). N=35-55 cells observed in 3 independent experiments. Bars: mean ± 95% CI. ****:p&lt;0.0001 by analysis of variance with Dunnett's multiple comparisons test. "
NCBI gene IDs:
"Ddr2: 18214
β-globin: 3043"
Figure legend: "B. FISH images and corresponding PDI measurements of mouse fibroblast cells treated with the indicated PMOs. Cyb5r3 is an APC-dependent RNA also enriched at protrusions. Arrows: peripheral Rab13 RNA. Arrowheads: perinuclear Rab13 RNA. Boxed regions are magnified in the insets. Note that Rab13 RNA becomes perinuclear in cells treated with PMOs against the GA-rich region. Scale bars: 10 μm. (4 μm in insets). ****: p&lt;0.0001 by analysis of variance with Dunnett's multiple comparisons test. N=40-90 cells observed in 3-6 independent experiments. Bars: mean ± 95% CI."
NCBI gene IDs:
"Cyb5r3: 109754
Rab13: 68328"
Figure legend: "C. Protrusion (Ps) and cell body (CB) fractions were isolated from cells treated with control-PMO or Rab13-PMO #2. The indicated RNAs were detected through nanoString analysis to calculate Ps/CB enrichment ratios (n=3; bars: mean ± s.e.m.). Note that only the distribution of Rab13 RNA is affected. **: p=0.01 by two-way ANOVA with Bonferroni's multiple comparisons test against the corresponding control. D. Levels of the indicated RNAs were determined using nanoString analysis from control- or Rab13 PMO #2-treated cells. (n=4; bars: mean ± s.e.m.). No significant differences were detected by two-way ANOVA against the corresponding controls. "
NCBI gene IDs:
"Rab13: 5872"
Figure legend: "A. Schematic showing %GA content and positions along the human RAB13 3'UTR targeted by the indicated PMOs. Red rectangles indicate the location of GA-rich motifs. Graphs present PDI measurements of RAB13 RNA (upper panel) or NET1 RNA (another APC-dependent RNA; bottom panel) detected in MDA-MB-231 cells treated with the indicated PMOs. PDI=1 indicates a diffuse distribution. p-values: **&lt;0.01, ***&lt;0.001, ****&lt;0.0001 by analysis of variance with Dunnett's multiple comparisons test. n.s.: non-significant. N=30-73 cells observed in 3-5 independent experiments. Bars: mean ± s.e.m.."
NCBI gene IDs:
"NET1: 10276
RAB13: 5872"
Figure legend: "B. Representative FISH images of cells treated with the indicated PMOs. Arrows point to peripheral RNA. Arrowheads point to perinuclear RNA. Boxed regions are magnified in the insets. Note that RAB13 RNA becomes perinuclear in cells treated with PMOs against the GA-rich region, while NET1 remains localized at protrusions. Scale bars: 10 μm (4 μm in insets)."
NCBI gene IDs:
"NET1: 10276
RAB13: 5872"
Figure legend: "C. RAB13 protein levels were measured by quantitative Western blot and normalized to total α-Tubulin or GAPDH levels (for representative blot, see Figure 5E). Relative levels in RAB13 PMO-treated compared to control are shown. No significant differences were detected by Kruskal-Wallis test with Dunn's multiple comparisons test. N=3-8. Bars: mean ± SD."
NCBI gene IDs:
"RAB13: 5872"
Figure legend: "A. Transwell migration of MDA-MB-231 cells treated with control PMOs or RAB13 PMOs (191+230). Cells reaching the bottom surface after 4hrs were counted. n=25 fields of view in each of 6 independent experiments. Bars: mean ± s.e.m.."
NCBI gene IDs:
"RAB13: 5872"
Figure legend: "B. Cells expressing Cherry-NLS, and treated with the indicated PMOs, were tracked every 5min for 10hrs to derive average migration speed. n=65 cells. Bars: mean ± s.e.m.."
NCBI gene IDs:
"Cherry-NLS: "
Figure legend: "D. Lifeact-GFP-expressing cells were treated with the indicated PMOs and imaged every minute over 1 hr. Sequential image frames, from a control cell, highlight edge retraction (red arrowheads) or protrusion (yellow arrowheads). Corresponding edge velocity is shown, with negative values indicating retraction and positive values indicating extension. Average protrusion and retraction speeds were calculated from n=11-13 cells. Bars: mean ± s.e.m..See also movies EV2 and EV3."
NCBI gene IDs:
"GFP: "
Figure legend: "G. Migration speed of RAB13 knockdown cells re-expressing GFP-RAB13 or GFP-RAB13 with a frameshift (fs) mutation at the beginning of the RAB13 coding region. 28-52 cells were analyzed. Bars: mean ± s.e.m.. Similar results were obtained in two additional independent experiments. Re-expressing cells were identified through GFP fluorescence and cells with similar, low GFP signal were tracked. All cells are expressing cherry-NLS for accurate tracking."
NCBI gene IDs:
"cherry-NLS: 
GFP: 
RAB13: 5872
RAB13: 68328"
Figure legend: "A. Widefield images of RAB13 immunofluorescence in MDA-MB-231 cells treated with control or RAB13 (191+230) PMOs and ratios of peripheral/perinuclear intensity. Scale bars: 10 μm. n=45-50 cells. Bars: mean ± s.e.m.. Similar results were observed in two additional independent experiments."
NCBI gene IDs:
"RAB13: 5872"
Figure legend: "B. Fluorescence images (projections of confocal slices throughout the cell height) of cells expressing GFP-RAB13 with the indicated UTRs. Note that in both cases the protein assumes indistinguishable distribution. Scale bars: 10 μm."
NCBI gene IDs:
"GFP: 
RAB13: 5872"
Figure legend: "A. Immunoprecipitation and Western blot analysis to detect proteins bound to GFP-RAB13 expressed from constructs carrying wt or ∆PMO RAB13 3'UTR. GFP-RAB13 (T22N) expresses a nucleotide-free mutant which binds tightly to putative GEFs. RABIF panel is also shown with adjusted contrast to reveal lower binding to wtGFP-RAB13. B. Quantification of RABIF binding to RAB13 from experiments as in (A). n=6. Bars: mean ± s.e.m.. "
NCBI gene IDs:
"GFP: 
RAB13: 5872"
Figure legend: "C. Active RAB13 pulldown assay from cells with CRISPR knockdown of RABIF using the indicated sgRNAs. n=3. Bars: mean ± s.e.m.."
NCBI gene IDs:
"CRISPR: 
RABIF: 5877"
Figure legend: "F. Representative RABIF-GFP PLA images and quantitations from cells expressing GFP-RAB13(T22N) carrying wt or ∆PMO RAB13 3'UTR. N&gt;70 cells. Bars: mean ± s.e.m.. Arrows indicate PLA signal at the cell periphery. Arrowheads indicate PLA signal within the cell body. Boxed regions are magnified in the insets. Scale bars: 10 μm; 4 μm in insets."
NCBI gene IDs:
"GFP: 
RAB13: 5872"
Figure legend: "B. Quantification of GFP RNA levels associating with RABIF in immunoprecipitation assays from the indicated cell lines. Note that even though RABIF binds several-fold more to RAB13(T22N) (see Figure 7A, B) it binds similarly to the wild-type and T22N RAB13 RNA, indicating that RABIF binds similarly to nascent RAB13. After translation, it is likely displaced upon GTP-loading of wild type RAB13, while it remains more stably bound to the nucleotide-free (T22N) form. N=6. Bars: mean ± s.e.m.. p-values: *&lt;0.05 by analysis of variance with Dunn's multiple comparisons test."
NCBI gene IDs:
"GFP: 
RAB13: 5872"
Figure legend: "Analysis of ER architecture and dynamics as a function of RNF26 in U2OS cells ectopically expressing mCherry-KDEL and transfected with either control siRNA (siC) or siRNAs targeting RNF26 (siRNF26-1 or siRNF26-2). B. Time-lapse imaging. Top panels: representative confocal fluorescence images of Cherry-KDEL (white) at the start of time-lapse t=0 (top). Middle panels: time color coded overlays of 60x1s frames, each assigned a different color LUT (white - same ER location across all frames; color - ER movement between frames). Bottom panels: overlays of twice eroded ER sheet masks (magenta) with mCherry-KDEL signal (green). Masks were generated using ImageJ software. Zoom-ins show select PN and PP regions. Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively."
NCBI gene IDs:
"mCherry: 
RNF26: 79102"
Figure legend: "I. CLIMP63 abundance as a function of RNF26. Top: representative immunoblot of U2OS cells transfected with the indicated siRNAs stained against endogenous CLIMP63 and Vinculin (loading control). Bottom: quantification of CLIMP63 abundance, normalized to Vinculin and expressed relative to control (siC=1.0), n=4 independent experiments. Data information: Graphs report mean or median (red line) of sample values (open circles). Significance was assessed using Student's t-test with error bars reflecting +/- SD)"
NCBI gene IDs:
"RNF26: 79102"
Figure legend: "J, K. Effect of RNF26 depletion on basal ER stress using IRE1-mediated XBP1 splicing as a readout. J. Relative abundance of RNF26 transcript normalized to GAPDH; n=3 independent experiments. All transcripts were detected by qPCR. Plots report values relative to control (siC=1.0). K. Quantification of IRE1-dependent XBP1 splicing. XBP-s and XBP1 transcripts isolated from U2OS cells transfected with the indicated siRNAs were detected by qPCR, normalized to GAPDH, and expressed as ratio, n=3 independent experiments. Data information: Graphs report mean or median (red line) of sample values (open circles). Significance was assessed using Student's t-test J and K with error bars reflecting +/- SD) p&lt;0.05; *** p&lt;0.001, ns: not significant."
NCBI gene IDs:
"IRE1: 2081
GAPDH: 2597
RNF26: 79102
XBP-s: 7494
XBP1: 7494"
Figure legend: "C, D. Proximity biotinylation of endogenous vimentin by 2HA-TurboID-RNF26, catalytically inactive point mutant (I382R), or RING domain truncation mutant (∆RING) in HeLa cells following 30 min incubation with biotin prior to lysis. C. Representative immunoblots of neutravidin precipitates and lysate inputs stained against HA and vimentin. D. Quantifications of vimentin biotinylation by 2HA-TurboID-RNF26 and mutants normalized to auto biotinylated HA-tagged RNF26 species and expressed relative to RNF26 WT, n=6 independent experiments performed in U2OS and HeLa cells. Data information: WCL: whole cell lysate. Graphs in D report mean (red line) and SEM (error bars) of sample values (open circles). Significance was assessed using Student's t-test; * p&lt;0.05; ** p&lt;0.01; *** p&lt;0.001."
NCBI gene IDs:
"HA: 
TurboID: 
RNF26: 79102"
Figure legend: "G. Proximity-based biotinylation of endogenous vimentin by 2HA-TurboID-I382R, -∆RING, and -I382R-∆C lacking amino acids 423-433. Immunoblots of neutravidin precipitates and lysate inputs against HA and vimentin are shown and are representative of three independent experiments. Data information: WCL: whole cell lysate."
NCBI gene IDs:
"HA: 
TurboID: "
Figure legend: "A-E. Analysis of RNF26 sequence determinants with respect to the intracellular distribution of RNF26 and its colocalization with vimentin filaments. A. Representative confocal fluorescence images of U2OS cells ectopically expressing RFP-RNF26 (green) wild-type (WT) or mutants (I382R, ∆RING, ∆C), fixed and immunostained against endogenous vimentin (magenta) and VAP-A (magenta). Indicated single channels and their corresponding color overlays are shown. Zoom-ins highlight PN regions. B. Colocalization (Manders') analysis of RFP-RNF26 and mutants overlap with vimentin. Plot reports n WT=38, n I382R=31, n ΔRING=34, n ΔC=22 cells analysed from three independent experiments. C. Colocalization (Manders') analysis of RFP-RNF26 and mutants with VAP-A. Plot reports n WT=29, n I382R=21, n ΔRING=17, n ΔC=16 cells analysed from three independent experiments. D. Perinuclearity of RFP-RNF26 as a function of its RING domain determinants. Plot reports n WT=72, n I382R=48, n ΔRING=37, n ΔC=28 cells analysed from three independent experiments. E. Intracellular distribution of vimentin as a function of RNF26 (mutant) overexpression. Plot reports n WT59, n I382R=58, n ΔRING=36, n ΔC=32 technical replicates from three independent experiments. Values capped at 10. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm. Statistical analyses were performed using Students' T-test (B, D error bars indicate mean -/+ SD) or Mann-Whitney U test (C, E error bars indicate median and 95% confidence interval); *** p&lt;0.001, ns: not significant."
NCBI gene IDs:
"RFP: 
RNF26: 79102"
Figure legend: "F, G. Analysis of vimentin cytoskeleton distribution as a function of RNF26. F. Representative confocal fluorescence images of control cells, cells silenced for RNF26 (si#1), or RNF26 KO cells. Zoom-ins highlight PN regions. G. Perinuclearity of vimentin in the presence or absence of RNF26. Plot reports n control=98, n siRNF26=132, n RNF26 KO=41 technical replicates from three independent experiments. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm. Statistical analyses were performed using Students' T-test Mann-Whitney U test error bars indicate median and 95% confidence interval); *** p&lt;0.001, ns: not significant."
NCBI gene IDs:
"RNF26: 79102"
Figure legend: "H. Vimentin abundance as a function of RNF26. Top: representative immunoblot of U2OS cells transfected with siRNF26#1 stained against endogenous vimentin and Vinculin (loading control). Bottom: quantification of vimentin abundance, normalized to Vinculin and expressed relative to control (siC=1.0), n=4 independent experiments. Statistical analyses were performed using Students' T-test error bars indicate mean -/+ SD) ; *** p&lt;0.001, ns: not significant."
NCBI gene IDs:
"RNF26: 79102"
Figure legend: "A-D. Effect of vimentin ablation on intracellular distribution of RNF26. A. Representative confocal images of parental and vimentin knockout (Vim KO) U2OS cells ectopically expressing GFP-RNF26 (green), fixed and immunostained against endogenous VAP-A (magenta). B. Validation of vimentin knockout. Immunoblots against endogenous vimentin and Vinculin (loading control) of a Vim KO clonal cell line and their parental U2OS cells are shown, representative of three independent experiments. Molecular weight markers are as indicated. C. Quantification of GFP-RNF26 signal distribution expressed as perinuclearity ratio. Graph reports n Parental = 73, n Vim KO = 43 technical replicates from three independent experiments. D. Colocalization (Manders') analysis of VAP-A overlapping with GFP-RNF26 (left plot) and vice versa (right plot). Plots report on subset of cells from data set in (B), same as in (C). Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Zoom-ins designate regions encompassing perinuclear and peripheral areas. All scale bars = 10µm. Graphs report mean (red line) of sample values (open circles). Statistical analyses were performed using Students' T-test (D Mann-Whitney U test (C error bars indicate median and 95% confidence interval); * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001."
NCBI gene IDs:
"Vim: 7431
vimentin: 7431"
Figure legend: "E, F. Effect of vimentin or RNF26 ablation on the intracellular distribution of endolysosomes. E. Representative confocal images of parental, RNF26 KO, vimentin KO #1 U2OS cells, vimentin KO#1 cells ectopically complemented with untagged wild type vimentin (Vim KO + Rescue), fixed and immunostained against endogenous LAMP1. F. Quantification of LAMP1 signal distribution expressed as perinuclearity ratio. Graph reports on n Parental = 145, n R26 KO = 64, n Vim KO#1 = 119, n Vim KO#1/Rescue= 32 technical replicates from three independent experiments.Values capped at 6. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Zoom-ins designate regions encompassing perinuclear and peripheral areas. All scale bars = 10µm. Graphs report mean (red line) of sample values (open circles). Mann-Whitney U test F error bars indicate median and 95% confidence interval); * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001."
NCBI gene IDs:
"R26: 79102
RNF26: 79102
Vim: 7431
vimentin: 7431"
Figure legend: "G, H. Effects of vimentin ablation on trafficking of extracellular materials to lysosomes. G. Time-lapse imaging. Representative confocal overlays of parental and vimentin KO#2 U2OS cells, stained with SiR-Lysosome (magenta) to mark late endocytic compartments and incubated with the cell impermeable dye SR101 (green), taken soon after the start (t=5 min) and at the end (t=120 min) of time-lapse are shown. Zoom-ins highlight select PN regions in overlay and single channels. H. Colocalization (Manders') of SiR-Lysosome overlapping SR101 at t=120 minutes following SR101 addition. Graph reports on n Parental = 71, n Vim KO = 69 technical replicates from 3 independent experiments. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Zoom-ins designate regions encompassing perinuclear and peripheral areas. All scale bars = 10µm. Graphs report mean (red line) of sample values (open circles). Statistical analyses were performed using Students' T-test H error bars indicate mean -/+ SD) ; * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001."
NCBI gene IDs:
"Vim: 7431
vimentin: 7431"
Figure legend: "Effect of vimentin ablation on ER organization and dynamics. Analysis of parental versus vimentin KO#1 U2OS cells ectopically expressing mCherry-KDEL. D. Time-lapse imaging. Left panels: representative confocal fluorescence images of Cherry-KDEL (white) at the start of time-lapse t=0 (top). Middle panels: time color coded (TCC) overlays of 60x1s frames. Right panels: overlays of twice eroded ER sheet masks (magenta) with mCherry-KDEL signal (green). Masks were generated using ImageJ software. Zoom-ins show select PN and PP regions. Scale bar = 10µm."
NCBI gene IDs:
"mCherry: 
vimentin: 7431"
Figure legend: "D, Consequences of RNF26, UBE2J1, and vimentin loss on ERQC formation. D. Representative confocal images of fixed U2OS cells transfected or genetically modified as indicated, treated in the absence (-) or presence (+) of tunicamycin (5ug/mL, O/N), fixed and immunostained against endogenous calnexin (white)."
NCBI gene IDs:
"RNF26: 79102
UBE2J1: 51465
vimentin: 7431"