EMBO/SourceData huggingface dataset:
Figure legend: "C. Interaction between GPx8 and caspase-4 in THP-1 cells in the presence or absence of priming LPS was demonstrated by the in situ proximity ligation assay (PLA) (red dots, labeled by arrowheads). GPx8-Myc stably expressing THP-1 were used to detect the interaction between GPx8-Myc and endogenous caspase-4. Untreated cells (Ctrl) or cells primed with LPS (primed LPS) were incubated with anti-Myc and anti-caspase-4 Abs, according to the manufacturer's instructions, and the nucleus was stained with DAPI (blue). Results were quantified by counting at least 5 different fields with an average of 300 cells. Reactions without primary Abs were used as the control for the PLA assay (PLA Ctrl). Data information: All data are representative of at least 3 independent experiments. *P &lt; 0.05; **P &lt; 0.01; *** P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 
Myc: "
Figure legend: "293T cells were co-transfected with vectors expressing FLAG tagged caspase-4 (WT or C258S) in addition to GPx8 expressing vectors. Cell lysate was immunoprecipitated by anti-FLAG Ab and subsequently analyzed by Western blots using anti-GPx8 or anti-FLAG Ab. (D) Complexes of GPx8 and caspase-4 were demonstrated by co-IP assays. Cysteine-dependent covalent interactions of GPx8 and caspase-4 (C258S) were analyzed under non-reducing and reducing conditions. Disulfide-linked complexes of Gpx8 and caspase-4 are indicated."
NCBI gene IDs:
"FLAG: 
GPx8: 
caspase-4: 12363"
Figure legend: "293T cells were co-transfected with vectors expressing FLAG tagged caspase-4 (WT or C258S) in addition to GPx8 expressing vectors. Cell lysate was immunoprecipitated by anti-FLAG Ab and subsequently analyzed by Western blots using anti-GPx8 or anti-FLAG Ab. (E) Interactions of GPx8 and caspase-4 in response to H2O2 treatment were demonstrated by co-IP assays of lysates prepared from 293T cells treated with H2O2 (200 μM)."
NCBI gene IDs:
"FLAG: 
GPx8: 
caspase-4: 12363"
Figure legend: "293T cells were co-transfected with vectors expressing FLAG tagged caspase-4 (WT or C258S) in addition to GPx8 expressing vectors. Cell lysate was immunoprecipitated by anti-FLAG Ab and subsequently analyzed by Western blots using anti-GPx8 or anti-FLAG Ab. (F) C79S, C108S, and C2S2 mutants of GPx8 abolished most of the binding to caspase-4 by co-IP assays."
NCBI gene IDs:
"FLAG: 
GPx8: 
caspase-4: 12363"
Figure legend: "C79 of GPx8 was essential for its inhibitory effect on noncanonical inflammasome activation. THP-1 cells stably expressing empty vector (EV), WT, or mutant GPx8 proteins were confirmed by immunoblotting (G)."
NCBI gene IDs:
"GPx8: "
Figure legend: "C79 of GPx8 was essential for its inhibitory effect on noncanonical inflammasome activation. (H and I) Cells were treated with 12-O-tetradecanoylphorbol 13-actate (PMA) (0.1 μM) and differentiated for 3 days then primed and transfected with LPS. Activation of the noncanonical inflammasome by different amounts of cytoplasmic LPS was verified by IL-1β (H) and LDH release (I). Data information: All data are representative of at least 3 independent experiments. In H and I, data are presented as the mean ± SD, n=4, technical repeats. *P &lt; 0.05; **P &lt; 0.01; *** P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: "
Figure legend: "A. Casp4C118S mutant partially abolished the covalent disulfide-link with GPx8. Interacting GPx8 were co-immunoprecipitated with FLAG-tagged caspase-4 (WT, C118S, C329S mutants) and analyzed under reducing (+DTT) or non-reducing (-DTT) conditions. Disulfide-linked complexes of GPx8 and caspase-4 are indicated. Fold change of complexes was normalized with internal controls and indicated below blots."
NCBI gene IDs:
"FLAG: 
Casp4: 12363
caspase-4: 12363"
Figure legend: "C118 of caspase-4 was required for the GPx8-mediated inhibitory effect. THP-1 cells stably expressing empty vector (EV), FLAG-tagged caspase-4 (WT, C258S, C118S mutants), or GPx8 (WT and C79S mutants) were confirmed by immunoblotting (B). Data information: All data shown are representative of at least 3 independent experiments."
NCBI gene IDs:
"FLAG: 
GPx8: 
caspase-4: 12363"
Figure legend: "C118 of caspase-4 was required for the GPx8-mediated inhibitory effect. (C-D) Casp4C118S completely abolished the inhibition of noncanonical inflammasome signaling in response to intracellular LPS. THP-1 cells were differentiated, primed, and transfected with LPS. Activation of the noncanonical inflammasome by different quantities of cytoplasmic LPS is confirmed by the production of IL-1β (C) and LDH (D). In C and D, data are presented as the mean ± SD, n=4, technical repeats. ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 
Casp4: 12363
caspase-4: 12363"
Figure legend: "(b) PC12 cells transfected with either 60Q or 60Q-HSC70bm. The presence of two HSC70bm motifs in 60Q-HSC70bm reduced the number of inclusions as compared to 60Q (scale bar, 20 μm). (c) Quantification of the PC12 cells with inclusions 24 h after transfection with either 60Q or 60Q-HSC70bm. 10 mM 3-methyladenine (3-MA), 10 μM pepstatin A and 10 μM leupeptin were added to inhibit macroautophagy, the proteases, cathepsins D and E, and cathepsin A, respectively. Bars in c represent relative mean values ± s.e.m. from four independent experiments, with levels of aggregation observed for 60Q normalized to a value of 1. Quantifications were performed by ArrayScan."
NCBI gene IDs:
"HSC70bm: 6622"
Figure legend: "(b) Confocal images of the 150Q Neuro2a cells transfected with R, RH, RS, RHS, RQ and RHQ. Molecules containing QBP1 co-localized with the tNHTT-150Q-EGFP inclusions (bar, 5 μm). Cells with inclusions were chosen to illustrate the co-localization of QBP1-containing molecules with tNHTT-150Q-EGFP."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(c) The expression of RQ and RHQ in 150Q Neuro2a cells decreased the accumulation of insoluble tNHTT-150Q-EGFP on the gel top. Note the shift of tNHTT-150Q-EGFP from an insoluble to a soluble form when RQ was co-expressed. The levels of tNHTT-16Q-EGFP were not affected. Full-length western blots are presented in Supplementary Figure 13."
NCBI gene IDs:
"150Q: 3064
HTT: 3064"
Figure legend: "(d) RHQ reduced the levels of aggregated and soluble forms of tNHTT-150Q-EGFP by 81.8% and 33.6%, respectively. WB, western blot. (e) Quantification of tNHTT-16Q-EGFP protein levels."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(a,b) Constructs identical to those described in Figure 2a, but lacking mRFP (representations of S, HS, Q, HQ are shown in Supplementary Fig. 3a) were co-transfected with tNHTT-16Q-EGFP (a) and tNHTT-150Q-EGFP (b) constructs into Neuro2a cells (bar, 4 μm)."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(c) Dot blot analysis of the lumenal fraction of purified lysosomes from Neuro2a cells co-transfected with tNHTT-60Q-EGFP and R, RS, RHS, RQ or RHQ. PNS, post-nuclear supernatant."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(d) Quantification of HTT translocation into lysosomes. Levels of HTT detected by EM48 (anti-huntingtin antibody) were normalized to levels of β-tubulin (in PNS) and levels of cathepsin D (in lysosomes)."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(e) Compound images generated by ArrayScan illustrating inclusion formation in 150Q Neuro2a cells transfected with R, RS, RHS, RQ and RHQ and shRNA specific for HSC70 and/or Lamp2a. (blue, Hoechst 33258; green, tNHTT-150Q-EGFP; red, tested molecule)."
NCBI gene IDs:
"HSC70: 15481"
Figure legend: "(f) RNAi of HSC70, Lamp2a or both alleviated the inhibitory effect of RHQ on the formation of inclusions containing tNHTT-150Q-EGFP inclusions."
NCBI gene IDs:
"HSC70: 15481
Lamp2a: 16784"
Figure legend: "(g) Western blot analysis of soluble polyQ protein in 150Q Neuro2a cells transfected with tested constructs and shRNA for HSC70 and/or Lamp2a. Full-length western blots are presented in Supplementary Figure 13."
NCBI gene IDs:
"HSC70: 15481
Lamp2a: 16784"
Figure legend: "(h) Silencing of HSC70, Lamp2a or both alleviated the inhibitory effect of RHQ on levels of soluble tNHTT-150Q-EGFP. Bars in d and f represent mean values ± s.e.m. from three independent experiments. Bars in h represent relative mean values ± s.e.m. from three independent experiments. For h, values for the R construct were arbitrarily set to 1."
NCBI gene IDs:
"HSC70: 15481
Lamp2a: 16784"
Figure legend: "(c) Western blot analysis of HTT aggregation (gel top, EM48 antibody), soluble HTT (1C2 antibody), and higher molecular polyQ complexes by agarose gel electrophoresis for resolving aggregates (AGERA). Full-length western blots are presented in Supplementary Figure 13. (d) Quantification of the gel top-detected aggregation (*, P = 0.093; **, P = 0.0027; ***, P = 0.0045). (e) Quantification of soluble HTT-polyQ (*, P = 0.034; **, P = 0.00003; ***, P = 0.0001)."
NCBI gene IDs:
"HTT: 3064"
Figure legend: "(B) Representative live-cell fluorescent images of HeLa cells co-transfected with EGFP-TECPR1 and LAMP1-mCherry and treated with 1 mM LLOMe for the indicated time. Scale bar = 10 µM."
NCBI gene IDs:
"EGFP: 
mCherry: 
LAMP1: 3916
TECPR1: 25851"
Figure legend: "(D) Representative live-cell fluorescent images of HeLa cells co-transfected with EGFP-TECPR1 and LAMP1-mCherry and treated as indicated. Scale bars = 10 µM. (E) Quantification of the fold change in EGFP-TECPR1 lysosomal fluorescence from D. Grey points represent individual cells from three independent experiments. Red points represent the means of individual experiments (n &gt; 25 cells per experiment). Bars represent the mean ± SD from the three experiments. Significance was determined from biological replicates using a one-way ANOVA with Tukey's multiple comparisons tests. ** p = &lt;0.005, **** p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"EGFP: 
mCherry: 
LAMP1: 3916
TECPR1: 25851"
Figure legend: "(B) Representative live-cell fluorescent images of HeLa cells co-transfected with the indicated EGFP-TECPR1 deletion mutant and LAMP1-mCherry before and after treatment with 1 mM LLOMe. Scale bars = 10 µM. To the right is the corresponding quantification of the fold change in EGFP-TECPR1 lysosomal fluorescence intensity after a 15-minute treatment with vehicle or 1 mM LLOMe. Grey points represent individual cells from three independent experiments."
NCBI gene IDs:
"EGFP: 
mCherry: 
LAMP1: 3916
TECPR1: 25851"
Figure legend: "(A,B) Representative live-cell fluorescent images of HeLa cells co-transfected with EGFP-TECPR1 and mCherry-Gal3 and treated with 1 mM LLOMe for the indicated time. Arrowheads in B indicate the appearance of TECPR1 (green arrow) and Gal3 (magenta arrow). Scale bars = 10 µM for A and 1 µM for B. (C) Quantification of TECPR1 and Gal3 recruitment to damaged lysosomes. Data are presented as mean ± SD from 5 independent experiments (each experiment represents a single cell with at least 3 individual lysosomes quantified)."
NCBI gene IDs:
"EGFP: 
mCherry: 
Gal3: 8484
TECPR1: 25851"
Figure legend: "(D) Representative confocal image of a HeLa cell transfected with EGFP-TECPR1, treated with LLOMe for 5 minutes, and immunostained for CHMP2A and LAMP1. Scale bars = 10 µm for whole image and 1 µm for insets. Fluorescence intensity profiles of the indicated channels across the dotted lines are shown in the lower subpanel. (E) Representative confocal images of a HeLa cells transfected with EGFP-TECPR1 and TagBFP-TMEM192 (a lysosomal/late endosomal protein), treated with 1 mM LLOMe for the indicated time, and immunostained for ALIX and Gal3. Scale bars = 10 µm."
NCBI gene IDs:
"EGFP: 
TECPR1: 25851"
Figure legend: "(A) Western blot analysis of LC3 lipidation status in wild type (WT), FIP200 KO and ATG16L1 KO HeLa cells treated with the indicated concentrations of LLOMe for 30 minutes."
NCBI gene IDs:
"ATG16L1: 55054
FIP200: 9821"
Figure legend: "(B) Quantification of LC3-II levels in HEK, HeLa and MEF WT and ATG16L1-deficient cell lines treated with and without 1 mM LLOMe for 30 minutes. Bars show mean ± SD from three biologically independent experiments represented as data points. Significance was determined from biological replicates using a Student's t tests. *p = 0.0177, ** p = 0.0082, *** p = 0.0001."
NCBI gene IDs:
"ATG16L1: 55054"
Figure legend: "(C) Western blot analysis of LC3 lipidation status in WT, ATG7 KO and ATG16L1 KO HeLa cells (top) and WT, ATG5-/- and ATG16L1Δ/Δ MEFs (bottom) treated with the indicated concentration of LLOMe for 30 minutes."
NCBI gene IDs:
"ATG16L1: 55054
ATG16L1: 77040
ATG5: 11793
ATG7: 10533"
Figure legend: "(F) Western blot analysis of lyso-IP samples collected from HeLa ATG16KO cells treated with or without 1 mM LLOMe for 30 minutes. Bars show mean ± SD from three biologically independent experiments represented as data points."
NCBI gene IDs:
"ATG16: 55054"
Figure legend: "(G) Representative live-cell fluorescent images of HeLa cells co-transfected with TagBFP-TMEM192, EGFP-ATG5 and mCherry-TECPR1, before and after a 15-minute treatment with 1 mM LLOMe. Scale bars = 10 µm for whole images and 2 µm for zoom. To the right is the corresponding quantification of the fold change in ATG5/TECPR1 lysosomal fluorescence intensity after a 15-minute treatment with vehicle or 1 mM LLOMe. Grey points represent individual cells from three independent experiments. Green/magenta points represent the means of individual experiments (n &gt; 25 cells per experiment)."
NCBI gene IDs:
"EGFP: 
mCherry: 
TagBFP: 
ATG5: 9474
TECPR1: 25851
TMEM192: 201931"
Figure legend: "(H) Western blot analysis of LC3 lipidation status in HEK TECPR1/ATG16L1 KO cells treated with 1 mMLLOMe for 30 minutes. To the right is the corresponding quantification of LC3-II protein levels. Bars show mean ± SD from three or four biologically independent experiments which are represented as data points."
NCBI gene IDs:
"ATG16L1: 55054
TECPR1: 25851"
Figure legend: "(A) Subjective assessment of hair depigmentation (score 0-5, with 0 = completely black and 5 = completely white) at 2 doses of either CNP520 or the BACE-1/-2 inhibitor NB-360 (mean ± SEM, n=8/group), statistical comparisons were made for every scoring day, versus vehicle using Kruskal-Wallis with Dunn's post-hoc test."
NCBI gene IDs:
"BACE-1: 23821"
Figure legend: "(A) Mouse forebrain Aβ40 levels in homozygous male and female APOE4-TR mice with and without CNP520 treatment, 4 hours after dosing, shown are mean ± SEM, n = 6/group."
NCBI gene IDs:
"APOE4: 348"
Figure legend: "(B) Distribution of APOE4 genotypes in the Phase IIa study, vs their baseline Aβ42/40 ratios. The dashed line at Aβ42/40 ratio of 0.09 represents a cutoff, which separates a normal ratio from an abnormally low ratio."
NCBI gene IDs:
"APOE4: 348"
Figure legend: "(C) Comparison of CSF Aβ40 reduction in APOE4 non-carriers and APOE4 carriers."
NCBI gene IDs:
"APOE4: 348"
Figure legend: "(F) Change of Aβ42/40 ratio for all participants with Aβ42/40 ≤ 0.09 at baseline; bars represent mean ± SD. Full circles: APOE4 carrier, open circles: APOE4 non-carrier."
NCBI gene IDs:
"APOE4: 348"
Figure legend: "Immunofluorescence staining of RANKL during in vitro adipogenesis of BMSCs from AdipoqCre; ranklfl/fl mice (Rankl-/-) and ranklfl/fl mice (Rankl+/+). Scale bar=50 μm."
NCBI gene IDs:
"Adipoq: 11450
Cre: 2777477
rankl: 21943
Rankl: 21943"
Figure legend: "Micro-CT and statistical analyses of BMD, BV/TV, Tb.N and Tb.Sp in trabecular bone from AdipoqCre; ranklfl/fl (Rankl-/-) and ranklfl/fl (Rankl+/+) mice at 4 weeks, 8 weeks and 16 weeks (n=5 independent biological replicates). Scale bar=1 mm. Data were compared using an unpaired t-test (** indicates P &lt; 0.01), error bars are standard deviations."
NCBI gene IDs:
"Adipoq: 11450
Cre: 2777477
rankl: 21943
Rankl: 21943"
Figure legend: "Representative images of TRAP staining of femur from AdipoqCre; ranklfl/fl (Rankl-/-) and ranklfl/fl (Rankl+/+) mice at 8 weeks. Scale bar=200 μm. Panel a1 and a4 indicate trabecular bone, a2 and a5 stand for growth plate, a3 and a6 denote cortical bone."
NCBI gene IDs:
"Adipoq: 11450
Cre: 2777477
rankl: 21943
Rankl: 21943"
Figure legend: "Immunofluorescence staining of osteocalcin from 8-week-old mice (n=6 mice in Rankl-/- group and 4 mice in Rankl+/+ group). Scale bar=50 μm. TB: trabecular bone. Statistical analyses of Ocn+ cells (n=6 independent microscopic vision fields from random mice samples). Data were compared using an unpaired t‐test (** indicates P &lt; 0.01), error bars are standard deviations."
NCBI gene IDs:
"Ocn: 12095
Rankl: 21943"
Figure legend: "Calcein staining of trabecular bone at 8 weeks. Scale bar=50 μm. TB: trabecular bone. Images are representative of 6 independent biological replicates in Rankl-/- and 5 replicates in Rankl+/+ group. Mineral apposition rate (MAR) of trabecular bone at 8 weeks.(n=6 mice in Rankl-/- group and 5 mice in Rankl+/+ group). Data were compared using an unpaired t‐test ( ** indicates P &lt; 0.01), error bars are standard deviations."
NCBI gene IDs:
"Rankl: 21943"
Figure legend: "Serum CTX-1, OCN and bone marrow RANKL levels from Rankl-/- and Rankl+/+ mice at 4 weeks (n=5 and 5), 8 weeks (n=5 and 6) and 16 weeks (n=5 and 5). Data were compared using an unpaired t‐test (* indicates P &lt; 0.05, ** indicates P &lt; 0.01), error bars are standard deviations.."
NCBI gene IDs:
"Rankl: 21943"
Figure legend: "Micro-CT analyses of sham and OVX model of AdipoqCre; ranklfl/fl (Rankl-/-) and ranklfl/fl (Rankl+/+) mice (n=5 independent biological replicates). Scale bar=1 mm. Data were compared using an unpaired t‐test (** indicates P &lt; 0.01), error bars are standard deviations."
NCBI gene IDs:
"Adipoq: 11450
Cre: 2777477
rankl: 21943
Rankl: 21943"
Figure legend: "Three-dimensional CT analyses of BMD, BV/TV, Tb.N, and Tb.Sp in trabecular bone from Vehicle (n=5 independent biological replicates) and ROS (n=6 independent biological replicates) model of AdipoqCre; ranklfl/fl (Rankl-/-) and ranklfl/fl (Rankl+/+) mice. Scale bar=1 mm. Data were compared using an unpaired t‐test (** indicates P &lt; 0.01), error bars are standard deviations.  "
NCBI gene IDs:
"Adipoq: 11450
Cre: 2777477
rankl: 21943
Rankl: 21943"
Figure legend: "D. Analysis of spore viability by yeast tetrad dissection. Diploid strains heterozygous for alleles of Nse6 (wt, 86-C, 177-C and 179-C) were sporulated. Isolated  spores were grown on YPD plates. Viable clones were tested for the marker cassette conferring resistance to G418 (marked by circles in green colours). Dead spores were marked by circles in red colours."
NCBI gene IDs:
"Nse6: 856760"
Figure legend: "C. Kaplan-Meier Survival plot showing low survival of chd7 mutants after 12 dpf (N=5)."
NCBI gene IDs:
"chd7: 569471"
Figure legend: "E. Acetylated tubulin staining  in 28 hpf controls (left) and mutants (right) showing severely affected outbranching of the trigeminal nerve (Vth cranial nerve). Notably, chd7-/- display reduced branching of the Vth cranial nerve (arrows) and axonal arborisation in the tectal  area. Graphs showing quantitative analyses of percentage (n=5) and mean total length  of peripheral projections (n=6) per zebrafish in controls and mutants (***p&lt;0.001; **p&lt;0.005, Student's t-test)."
NCBI gene IDs:
"chd7: 569471"
Figure legend: "C-H Total number of GABAergic neurons  (GFP+ cells) in (C, D) the optic tectum (OT) and cerebellum (CB) regions of 5 dpf wild-type and chd7 mutant fish (n=16; ****p&lt;0.0001; Student's unpaired t-test), (E, F) the hypothalamus (hyp) region (n=10; *p=0.0182; Student's t-test) and (G, H) the telencephalon (tel) (n=7; *p=0.0347; Student's t-test)."
NCBI gene IDs:
"chd7: 569471"
Figure legend: "A. qPCR analysis of PAQR3 expression in a CHD7-mutation positive patient compared to parental controls set (N=4; ****p&lt;0.0001; Student's t-test). Fold change was calculated according to the 2(−ΔΔCt) method, using HPRT1 and RPS1  as housekeeping genes for normalization. All data were expressed as mean fold change ± SD across replicates, relative to control parents set to 1 (dotted line), N is the number of experimental repeats."
NCBI gene IDs:
"RPS1: 
CHD7: 55636
HPRT1: 3251
PAQR3: 152559"
Figure legend: "C. ChIP-qPCR assays in LCLs showing decreased occupation of CHD7 on the PAQR3 proximal promoter in a CHD7-mutation positive patient (N=3) compared to parental controls (N=6); *p&lt; 0.05; Student's t-test, All data were expressed as mean fold change ± SD, N is the number of experimental repeats."
NCBI gene IDs:
"CHD7: 55636
PAQR3: 152559"
Figure legend: "C-D. Overexpression of paqr3b mRNA improve the number  of GABAergic neurons in 3dpf chd7  mutant fish (n=7 for chd7-/- and n=11 for chd7-/- + paqr3b  mRNA; ****p&lt;0.0001; **p&lt;0.05; One-way ANOVA). Of note, the rescue experiment was performed at 3 dpf  given the transient nature of mRNA."
NCBI gene IDs:
"chd7: 569471
paqr3b: 560764"
Figure legend: "B. Lifespan analyses of chd-7(gk290) mutants (n=339; red) compared to WT (n=444; black). Log-rank test was performed for statistical analyses. ***p &lt; 0.001."
NCBI gene IDs:
"chd-7: 172079"
Figure legend: "D-F. Example pictures of the GABAergic nervous system cell bodies, commissures, axonal gaps and axonal breaks larger than 50 µm per worm in chd-7(gk290) mutants expressing unc-47p::mCherry."
NCBI gene IDs:
"chd-7: 172079"
Figure legend: "O. Representative images  of GABAergic neurons and total number of GABAergic neurons (GFP+ cells) in the optic tectum  (OT) and cerebellum (CB) regions of wild-type, chd7 mutant and ephedrine-treated fish (n=9; ****p&lt;0.0001; One-way ANOVA). Treatment with ephedrine ameliorated the number of GABAergic neurons."
NCBI gene IDs:
"chd7: 569471"
Figure legend: "(A) The hair coats of Sirt7−/− and Sirt7+/+ mice were clipped on P60 in the mid second telogen phase. Images were captured at P60, P120 and P150."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(B) The postnatal day when the hair coat recovered by 25%, 50%, 75% and 100% in shaved Sirt7−/− and Sirt7+/+ mice. n = 6 mice/genotype. Box-and-whisker plots: mid-line, median; box, 25th and 75th percentiles; whiskers, minimum and maximum."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(C) H&amp;E staining of the skin in unshaved Sirt7−/− and Sirt7+/+ mice on P90 and P130."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(D) Representative immunofluorescence images showing Ki67 and p-cadherin (Pcad, labeling HG cells) expression in Sirt7−/− and Sirt7+/+ HFs on P74 and P90. The HF boundary and dermal papilla (DP) is denoted by the dotted and dashed lines, respectively."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(F) Telogen duration in Sirt7−/− and Sirt7+/+ mice. n = 6 mice per genotype. Box-and-whisker plots: mid-line, median; box, 25th and 75th percentiles; whiskers, minimum and maximum."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(G) The hair coats of Sirt7-TG and WT mice were clipped on P45 and images were captured on P56 and P90."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(H) The postnatal day when the hair coat recovered by 25%, 50%, 75% and 100% in shaved Sirt7-TG and WT mice. n = 6 mice per genotype. Box-and-whisker plots: mid-line, median; box, 25th and 75th percentiles; whiskers, minimum and maximum."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(I) Representative immunofluorescence images showing Ki67 and Pcad expression in Sirt7-TG and WT HFs at P60 and P65. The HF boundary and DP is noted by the dotted and dashed lines, respectively."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(K) H&amp;E staining of skin tissues in unshaved Sirt7-TG and WT mice on P65."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(L) Telogen duration in Sirt7-TG and WT mice. n = 6 mice per genotype. Box-and-whisker plots: mid-line, median; box, 25th and 75th percentiles; whiskers, minimum and maximum."
NCBI gene IDs:
"Sirt7: 209011"
Figure legend: "(C) The hair coats of Sirt7f/f and Sirt7f/f;K15-Cre mice were clipped at P60 and images were captured on P60, P125 and P150."
NCBI gene IDs:
"Cre: 2777477
K15: 16665
Sirt7: 209011"
Figure legend: "(D) The postnatal day of hair coat recovery by 25%, 50% 75% and 100% in shaved Sirt7f/f and Sirt7f/f;K15-Cre mice. n = 6 mice per genotype. Box-and-whisker plots: mid-line, median; box, 25th and 75th percentiles; whiskers, minimum and maximum."
NCBI gene IDs:
"Cre: 2777477
K15: 16665
Sirt7: 209011"