EMBO/SourceData huggingface dataset:
Figure legend: "E4.5 chimeric embryos stained for tdTomato and DAPI.   Quantification of donor cells' contribution in E4.5 blastocysts, Mann-Whitney test, data represent mean ± SD; Ronin fl/del n embryos = 26, Ronin del/del n embryos = 27.   Diapause embryos stained for tdTomato and DAPI.   Quantification of donor cells' contribution in diapause embryos, unpaired Student's t test, data represent mean ± SD, Ronin fl/del n embryos = 4, Ronin del/del n embryos = 12.   E5.5 chimeric embryos stained for tdTomato and DAPI.   Quantification of donor cells' contribution in E5.5 embryos, unpaired Student's t test, data represent mean ± SD, Ronin fl/del n embryos = 21, Ronin del/del n embryos = 15.   Reactivated E5.5 chimeric embryos stained for tdTomato and DAPI.   Quantification of donor cells' contribution in reactivated E5.5 embryos, Mann-Whitney test, data represent mean ± SD, Ronin fl/del n embryos = 17, Ronin del/del n embryos = 12.   "
NCBI gene IDs:
"Ronin: 59016"
Figure legend: "A Immunoblot analysis of the knockdown of exogenous USP19 in 293T cells expressing Flag-USP19 (top) or endogenous USP19 in HeLa cells (bottom) treated with USP19-specific siRNA or scrambled (Scr) siRNA."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "B Representative images of GFP-LC3B puncta in HeLa-GFP-LC3B cells transfected with USP19-specific siRNA or scrambled siRNA during growth in normal medium (DMEM) or EBSS medium for 3 h. Arrows denote representative autophagosomes. Scale bar, 200 μm."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "C Quantification of GFP-LC3B puncta in HeLa-GFP-LC3B cells transfected with USP19-specific siRNA or scrambled siRNA. Bars represented mean ± standard error of the mean (SEM) of triplicate samples (20 cells per sample). *p &lt; 0.05, **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "D Immunoblot detection of the relative accumulation of LC3 I and LC3 II in HeLa cells transfected with USP19-specific siRNA or scrambled siRNA with rapamycin (250 nM) treatment for 12 h."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "E HeLa cells were transfected with USP19-specific siRNA or scrambled siRNA and treated with indicated concentration of rapamycin for 12 h, p62 levels were detected by immunoblot."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "F Human peripheral blood mononuclear cells (PBMCs) transfected with control or USP19-specific siRNA were treated with rapamycin (250 nM) for 18 h, the lysates were analyzed with each antibody."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "H Flag-USP19 inducible HeLa cells were treated with 200 ng/ml doxycycline (Doxy) for overnight to induce the expression of Flag-USP19. The protein levels of p62 were analyzed after rapamycin treatment for 12 h at indicated concentrations."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "I GFP-LC3B stably expressing HeLa cells were transfected with pcDNA3.1 empty vector or plasmid expressing the wild-type and the mutant forms of USP19 (CS or CS/HA). Pictures in (B) and (I) were taken using Leica DMI3000 B microscope with a×100 oil-immersion objective. Scale bar, 200 μm.J Average GFP-LC3B puncta per cell were calculated. Bars represented mean ± SEM of triplicate samples (20 cells per sample). *p &lt; 0.05, **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "A 293T cells were transfected with plasmids encoding HA-USP19 and Flag-tagged Beclin-1 complex members (Flag-ATG14L, Flag-VPS15, Flag-Beclin-1, Flag-VPS34, Flag-UVRAG and Flag-AMBRA1), followed by immunoprecipitation (IP) with anti-Flag beads and immunoblot analysis with anti-HA. Throughout was the immunoblot analysis of whole-cell lysates (WCL) without immunoprecipitation."
NCBI gene IDs:
"AMBRA1: 55626
ATG14L: 22863
Beclin-1: 8678
VPS34: 5289
VPS15: 30849
UVRAG: 7405"
Figure legend: "B, C Extracts of A549 cells (B) or human PBMCs (C) incubated with EBSS for various time points (above lanes) were subjected to immunoprecipitation with anti-Beclin-1 and immunoblot analysis with indicated antibodies (shown on the left)."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "F Co-immunoprecipitation and immunoblot analysis of 293T cells transfected with deletion mutants of USP19 plasmid along with vector encoding Flag-Beclin-1."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "G Co-immunoprecipitation and immunoblot analysis of 293T cells transfected with deletion mutants of Beclin-1 plasmid along with vector encoding HA-USP19."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "C BECN1 mRNA levels of the same sample (B) were detected by real-time PCR. Bars represented mean ± SEM of triplicate samples."
NCBI gene IDs:
"BECN1: 8678"
Figure legend: "D Human PBMCs were transfected with USP19-specific or scramble siRNA and treated with rapamycin (250 nM) for 18 h. The extracts were analyzed by immunoblot with indicated antibodies."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "E Wild-type (WT) and USP19 KO A549 cells were cultured in EBSS for indicated time points, and the protein expression levels of Beclin-1 and ULK1 were detected by immunoblot."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "F USP13 KO HEK293T cells were transfected with plasmids encoding Myc-USP5, Myc-USP19 and Myc-USP38 and the cellular extracts were analyzed by immunoblot."
NCBI gene IDs:
"USP13: 8975
USP19: 10869
USP38: 84640
USP5: 8078"
Figure legend: "G 293T cells transfected with plasmid expressing Flag-Beclin-1 and empty vector or Myc-tagged USP19 (WT, CS, CS/HA or ΔTMD), the lysates were analyzed by immunoblot."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "H 293T cells were transfected with plasmid encoding Flag-Beclin-1 and treated with CHX (100 μg/ml) alone or with MG132 (10 μM), 3MA (10 mM), CQ (50 μM) as combination for 6 h or transfected with the HA-USP19 plasmid. The cell lysates were analyzed by immunoblot."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "A HeLa cells were transfected with USP19 siRNA or scrambled siRNA and protein extracts were harvested after MG132 (10 μM) treatment for 3 h. Protein extracts were immunoprecipitated using anti-Beclin-1 antibody or with IgG as a negative control and analyzed by immunoblot using anti-ubiquitin and anti-Beclin-1 antibodies."
NCBI gene IDs:
"Beclin-1: 8678
USP19: 10869"
Figure legend: "B Lysates of 293T cells transfected with plasmid expressing Flag-Beclin-1 and HA-tagged ubiquitin (HA-Ub (wild type), K6-linked-Ub, K11-linked-Ub, K27-linked-Ub, K29-linked-Ub, K33-linked-Ub, K48-linked-Ub or K63-linked-Ub), together with the empty vector or expression vector of Myc-USP19 and treated with MG132 (10 μM) for 3 h were immunoprecipitated with anti-Flag and immunoblotted with anti-HA."
NCBI gene IDs:
"ubiquitin: 7311///6233///7316///7314
USP19: 10869"
Figure legend: "C HeLa cells were transfected with USP19-specific siRNA and plasmid encoding Flag-Beclin-1, plus K11-linked-Ub. Protein was harvested after MG132 (10 μM) treatment for 3 h and immunoprecipitated with anti-Flag and immunoblotted with anti-HA."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "D 293T cells were transfected with plasmid for Flag-Beclin-1 and HA-K11-Ub, together with the empty vector or expression vector for Myc-USP19 (WT, CS or CS/HA) and treated with MG132 (10 μM). Cell lysates were subjected to immunoprecipitation with anti-Flag and immunoblot analysis with anti-HA."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "E Purified ubiquitinated Beclin-1 was incubated with immunopurified Flag-USP19 in vitro in deubiquitinating buffer. The immunoblot was probed with anti-HA."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "A Lysates of 293T cells transfected with plasmid for Flag-Beclin-1 and HA-K11-linked-Ub, together with empty vector or expression vector for Myc-USP5 or Myc-USP19 were immunoprecipitated with anti-Flag and immunoblotted with anti-HA."
NCBI gene IDs:
"USP19: 10869
USP5: 8078"
Figure legend: "B Immunoprecipitation and immunoblot analysis of 293T cells transfected with vectors expressing Flag-Beclin-1 and HA-K11-linked ubiquitin."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "C 293T cells were transfected with Flag-Beclin-1 (WT or K437R) and treated with CHX (100 μg/ml) for indicated time points. The expression levels of WT and K437R Flag-Beclin-1 were analyzed by immunoblot.D Quantification of the expression levels of WT and K437R Flag-Beclin-1."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "E Immunoblot analysis of protein extracts of 293T cells transfected with empty vector or vector for HA-USP19, together with plasmid expressing WT or K427R, K430R or K437R mutant of Flag-Beclin-1."
NCBI gene IDs:
"Beclin-1: 8678
USP19: 10869"
Figure legend: "F HeLa cells transfected with BECN1 sgRNA and restored with WT and K437R Flag-Beclin-1 were stained by anti-LC3 antibody. Scale bar, 200 μm.G Quantification of LC3 puncta shown in (F). Bars represented mean ± SEM of triplicate samples. *p &lt; 0.05, **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"Beclin-1: 8678
BECN1: 8678"
Figure legend: "A Luciferase activity in 293T cells transfected with an ISRE (left) or IFN-β (right) promoter-driven luciferase reporter and together with plasmid encoding RIG-I (N) or cGAS and STING."
NCBI gene IDs:
"Luciferase: 
luciferase: 
IFN-β: 3456"
Figure legend: "B Protein lysates of WT and USP19 KO A549 cells infected with SeV at indicated time points were immunoblotted with indicated antibodies."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "C RT-PCR analysis of gene transcription in WT and USP19 KO A549 cells after infection with SeV at indicated time points."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "D Phase-contrast microscopy (PH) and fluorescence microscopy images of WT and USP19 KO A549 cells infected with VSV-eGFP at an MOI of 0.01 for the indicated time length. Scale bar, 50 μm."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "E Human PBMCs were transfected with control or USP19-specific siRNA, followed by treatment with influenza A/Puerto Rico/8/34 (H1N1) (PR8) (MOI = 5) at different time points, the lysates were analyzed with each antibody."
NCBI gene IDs:
"USP19: 10869"
Figure legend: "F Human PBMCs were transfected with control or USP19-specific siRNA were infected with H1N1 virus (MOI = 5) at different time points. Relative expression levels of IFNB, ISG15, ISG54, ISG56 mRNA and H1N1 nucleoprotein (NP) RNA were measured by real-time PCR."
NCBI gene IDs:
"ISG56: 3434
ISG54: 3433
IFNB: 3456
ISG15: 9636
NP: 956531
nucleoprotein: 956531
USP19: 10869"
Figure legend: "G Relative expression levels of IFNB mRNA and interferon-stimulated genes, including ISG54 and ISG56 in WT, BECN1, ATG5 and SQSTM1 KO cells with or without USP19."
NCBI gene IDs:
"ATG5: 9474
BECN1: 8678
ISG56: 3434
ISG54: 3433
IFNB: 3456
SQSTM1: 8878
USP19: 10869"
Figure legend: "H, I Luciferase activity in WT and BECN1 KO 293T cells transfected with an ISRE (H) or IFN-β (I) promoter-driven luciferase reporter after infection with SeV for indicated time points."
NCBI gene IDs:
"Luciferase: 
luciferase: 
BECN1: 8678
IFN-β: 3456"
Figure legend: "A Luciferase activity in WT and BECN1 KO 293T cells transfected with an ISRE (left) or IFN-β (right) promoter-driven luciferase reporter after infection with SeV for 12 h."
NCBI gene IDs:
"Luciferase: 
luciferase: 
BECN1: 8678
IFN-β: 3456"
Figure legend: "B WT and BECN1 KO 293T cells were infected with SeV for indicated time points, then protein extracts were analyzed by immunoblot using the indicated antibodies."
NCBI gene IDs:
"BECN1: 8678"
Figure legend: "C Protein extracts of THP-1 cells infected with SeV for various time points (upper lanes) were subjected to immunoprecipitation with anti-Beclin-1 and immunoblot analysis with antibodies shown on the left."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "E 293T cells were transfected with vectors encoding HA-Beclin-1 and Flag-MAVS or Flag-MAVSΔCARD, followed by IP with anti-Flag beads and immunoblot analysis with anti-HA."
NCBI gene IDs:
"MAVS: 57506"
Figure legend: "F Co-immunoprecipitation and immunoblot analysis of WT and BECN1 KO 293T cells transfected with HA-MAVS plasmid along with vector encoding Flag-RIG-I (N) in the absence or presence of USP19."
NCBI gene IDs:
"BECN1: 8678
RIG-I: 23586
USP19: 10869"
Figure legend: "G Protein extracts of scramble or USP19-specific siRNA treated 293T cells transfected with HA-MAVS plasmid along with vector encoding for Flag-RIG-I (N) in the absence or presence of Beclin-1 were subjected to immunoprecipitation and immunoblot analysis."
NCBI gene IDs:
"Beclin-1: 8678
RIG-I: 23586
USP19: 10869"
Figure legend: "H Luciferase activity in 293T cells transfected with scramble or USP19-specific siRNA and ISRE luciferase reporter after SeV infection for indicated time points with or without Beclin-1."
NCBI gene IDs:
"Luciferase: 
luciferase: 
Beclin-1: 8678
USP19: 10869"
Figure legend: "I Luciferase activity in 293T cells transfected with scramble or USP19-specific siRNA and IFN-β luciferase reporter after SeV infection for indicated time points with or without Beclin-1."
NCBI gene IDs:
"Luciferase: 
luciferase: 
Beclin-1: 8678
IFN-β: 3456
USP19: 10869"
Figure legend: "A, B Immunofluorescence  of PAR-3  (A) and proportions of mESC  clusters with a strong PAR-3 centre (B) in wild-type (W4) and E-cadherin  knock-out (Cdh1  KO) mESCs  at 24 hrs in Matrigel. Data information: Data are presented as means ± SEM in (B) n = 3 experiments in (B) Two-way ANOVA analysis in (B), P values were listed in the graphs. scale bars: 10 µm."
NCBI gene IDs:
"Cdh1: 12550
E-cadherin: 12550"
Figure legend: "C Line-scan profiles of PAR-3   at the cell-cell interface in wild-type control, mitomycin C  treated and Cdh1  KO control, mitomycin C treated 2-cell doublets. D Ratio of  PAR-3  pixel intensity values at central and surrounding regions in &gt;2-cell mESC  clusters. Data information: individual and mean-valued line-scans in (C) individual cell cluster values (small dots), mean experimental values (large dots) and means of 3 experiments (bars) ± SEM in (D) 17-50 cell clusters were analysed for each column in every experiment; 15 doublets for each condition in (C); 17-30 cell clusters were analysed for each condition in every experiment in (D); Two-way ANOVA analysis in (D), P values were listed in the graphs. scale bars: 10 µm."
NCBI gene IDs:
"Cdh1: 12550"
Figure legend: "E, F Representative images of  PAR-3 immunofluorescence  (E) and line-scan profiles of PAR-3 at the cell-cell interface (F) in WT homogeneous or WT (ES-E14) /Cdh1  KO chimeric mESC  2-cell doublets. *, WT mESCs. Data information: individual and mean-valued line-scans in (F); 15 doublets for each condition in (F)"
NCBI gene IDs:
"Cdh1: 12550"
Figure legend: "G, H Representative images of ZO-1 puncta and Golgi apparatus  (G) and proportions of mESC  doublets with central ZO-1 puncta or polarised Golgi apparatus  (H) in WT and Cdh1  KO mESC  doublets. Data information: Data are presented as means ± SEM in (H); student's t-test analysis in (H); P values were listed in the graphs. scale bars: 10 µm."
NCBI gene IDs:
"Cdh1: 12550"
Figure legend: "A-C Immunofluorescence of PAR-3 and P-cadherin (A), proportions of mESC   clusters with a positive PAR-3  centre (B) and line-scans of PAR-3 at the cell-cell interface (C) in control, P-cadherin  knock-down by RNAi, mitomycin  treated and P-cadherin knock-down mitomycin  treated W4 mESC  doublets cultured for 24 hours in Matrigel. Data information: Data are presented as means ± SEM in (B) ; individual and mean-valued line-scans in (C) n = 3 experiments in (B), 15-21 clusters were analysed for each column in every experiment; 15-21 line-scans in (C) Two-way ANOVA analysis in (B) P values were listed in the graphs. scale bars: 5 µm."
NCBI gene IDs:
"P-cadherin: 12560"
Figure legend: "D-F Immunofluorescence  of PAR-3  and JAM-A (D), proportions of mESC  clusters with a positive PAR-3 centre (E) and line-scans of PAR-3 at the cell-cell interface (F) in control, JAM-A knock-down by RNAi, mitomycin  treated and JAM-A knock-down mitomycin  treated W4 mESC  doublets cultured for 24 hours in Matrigel. Data information: Data are presented as means ± SEM in (E), individual and mean-valued line-scans in (F) n = 3 experiments in (E), 15-21 clusters were analysed for each column in every experiment; 15-21 line-scans in (F), Two-way ANOVA analysis in (E), P values were listed in the graphs. scale bars: 5 µm."
NCBI gene IDs:
"JAM-A: 16456"
Figure legend: "G-I Immunofluorescence  of PAR-3 and Nectin-2 (G), proportions of mESC  clusters with a positive PAR-3 centre (H) and line-scans of PAR-3  at the cell-cell interface (I) in control, Nectin-2 knock-down by RNAi, mitomycin  treated and Nectin-2  knock-down mitomycin  treated W4 mESC  doublets cultured for 24 hours in Matrigel. Data information: Data are presented as means ± SEM in (H); individual and mean-valued line-scans in (I). n = 3 experiments in (H), 15-21 clusters were analysed for each column in every experiment; 15-21 line-scans in (I). Two-way ANOVA analysis in (H); P values were listed in the graphs. scale bars: 5 µm."
NCBI gene IDs:
"Nectin-2: 19294"
Figure legend: "A, B Immunofluorescence  of PAR-3 (A) and proportions of mESC  doublets with polarised  PAR-3 (B) in wild-type (ES-E14) and E-cadherin knock-out (Cdh1  KO) cells at 30 hours in 0.5% agarose. Data information: Data are presented as means ± SEM in (B); n = 3 experiments in (B) Two-way ANOVA analysis in (B) P values were listed in the graphs. scale bars: 5 µm."
NCBI gene IDs:
"Cdh1: 12550
E-cadherin: 12550"
Figure legend: "D Percentage of cell clusters with different cavities relative to total cell clusters at different  time points. The analysis was compared between the closed/rosette category among the conditions. E Percentage of cell clusters with closed cavities relative to total cell clusters with cavities calculated from (D). Data information: Data are presented as means ± SEM in (D) &amp; (E). n = 20 (48 hours WT &amp; Cdh1  KO), 30 (72 hours WT) and 32 (72 hours Cdh1  KO) images in (A); 3 experiments in (D) &amp; (E), at least 25 clusters were analysed for each column in every experiment. Two-way ANOVA analysis in (D) &amp; (E); P values were listed in the graphs."
NCBI gene IDs:
"Cdh1: 12550"
Figure legend: "Human  first-trimester trophoblast cell line Sw.71 (left panel) and human primary culture (right panel) trophoblast  cells were infected with ZIKV  (MOI=2) for 1 h and refreshed with regular media over  time. RNA was collected for measuring gene expression by qRT-PCR. Data represent as mean ± SEM; n=4 biological replicates. NT, no treatment group; HPC, human primary culture. (B) IFNα  is not highly induced in response to ZIKV  infection in trophoblast cells.  IFNα mRNA expression was inhibited in the first 24 h.p.i. and maintained at low level during  ZIKV infection. Difference not significant (n.s.) by One-way ANOVA."
NCBI gene IDs:
"IFNα: 3439"
Figure legend: "Human first-trimester trophoblast  cell line Sw.71 (left panel) and human primary culture  (right panel) trophoblast  cells were infected with ZIKV  (MOI=2) for 1 h and refreshed with regular media over  time. RNA was collected for measuring gene expression  by qRT-PCR. Data represent as mean ± SEM; n=4 biological replicates. NT, no treatment group; HPC, human primary culture. (C) ZIKV infection induced a  time-dependent increase in IFNβ mRNA expression in Sw.71  and HPC. *p &lt; 0.05, **p &lt; 0.01 by One-way ANOVA."
NCBI gene IDs:
"IFNβ: 3456"
Figure legend: "Sw.71 (left panel) and HPC cells (right panel) were treated with  IFNβ to detect ISG20 mRNA expression. Data represent as mean ± SEM; n=3 biological replicates. NT, no treatment group; HPC, human primary culture. (A) Sw.71 and HPC cells were treated with different  doses of IFNβ (3, 30, 300 ng/ml) for 8 h and RNA were collected for determining ISG20 mRNA expressions  by qRT-PCR. Note the increase of ISG20 mRNA expression in a dose-dependent manner. *p &lt; 0.05, **p &lt; 0.01 by One-way ANOVA. (B) Sw.71  and HPC cells were treated with 300ng/ml IFNβ over  time and RNA was collected for determining ISG20 mRNA expressions by qRT-PCR. Note the increase of ISG20 mRNA expression in a time-dependent manner. *p &lt; 0.05 by One-way ANOVA. (C) "
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(B) No  ISG20 protein expression in ISG20-/- Sw.71  in ZIKV infection. Sw.71 and ISG20-/- Sw.71 cells were infected with  ZIKV  (MOI=2) for 1 h and refreshed with regular media for 48 h, and proteins were collected for western blot  analysis.  β-actin served as a loading control."
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(C) Higher  ZIKA titer was shown in ISG20-/- Sw.71 cells. Sw.71 and  ISG20-/- Sw.71 cells were infected with ZIKV  (MOI=2) for 1 h and refreshed with regular media for 48 h, and  RNA  was collected for determining the viral titer and gene expression by qRT-PCR. Data represent as mean ± SEM; n=4 biological replicates; *p &lt; 0.05 by Student's t-test."
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(D) More viral shedding in  ZIKV-infected  ISG20-/- Sw.71  cell culture supernatant. Supernatant from ZIKV-infected Sw.71  and  ISG20-/- Sw.71 cells were collected and plaque assay was performed using Vero  cells. A representative  plaque assay picture is presented. Note that more plaques were formed in Vero  cells by incubating with ZIKV-infected ISG20-/- Sw.71  cell culture supernatant. Scale bar=130μm. (E) Plaques were counted and  plaque forming-unit (pfu) ratio was calculated for the comparison of the viral titers. Data represent as mean ± SEM; n=3 biological replicates. *p &lt; 0.05 by Student's t-test. "
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(B) ISGs mRNA expression stimulated by IFNβ  in Sw.71 and ISG20-/- Sw.71 trophoblast cells. Sw.71 and ISG20-/- Sw.71 cells were treated with 30 ng/ml IFNβ for 24 h, and RNA was collected for determining the ISGs gene expression  by qRT-PCR. Data represent as mean ± SEM; n=3 biological replicates; *p &lt; 0.05, **p &lt; 0.01 by Student's t-test against control."
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(D)  IFNβ pre-treatment significantly prevented trophoblast  cells from ZIKV infection, however, this protection was evidently attenuated due to lack of ISG20. Sw.71 and ISG20-/- Sw.71  cells were pre-treated with or without 30 ng/ml IFNβ for 24 h, followed by ZIKV infection (MOI=2) for 1 h and refreshed with regular media for 24 h, and  RNA was collected to determine the viral titers by qRT-PCR. Data represent as mean ± SEM; n=4 biological replicates; *p &lt; 0.05 by Student's t-test."
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "(D) ISG20 secretion in the positive clone supernatant significantly decreased ZIKV infection in  trophoblast  cells. Supernatants from negative and positive clones were collected and added to ISG20-/- Sw.71 trophoblast  cells together with ZIKA  virus (MOI=2) for 1 h, followed by refreshing with new growth media for 48 h.  RNA was then collected to determine viral titers by qRT-PCR. Data represent as mean ± SEM; n=3 biological replicates; **p &lt; 0.01 by Student's t-test."
NCBI gene IDs:
"ISG20: 3669"
Figure legend: "A In apoptotic Drp1 knockdown U2OS cells, cytochrome c release is delayed. Confocal overview image of an apoptotic Drp1 knockdown cell labelled with antibodies against Tom20 (red), cytochrome c (blue) and Bax (green). Large image: Overlay of all three channels. Small images: Magnifications of the area indicated by the rectangle in the large image. Shown are the individual channels, as indicated. The arrows point to mitochondria that had not released cytochrome c; the asterisks denote mitochondria that had released cytochrome c. Scale bars: 10 µm (overview) and 2 µm (magnifications)."
NCBI gene IDs:
"Drp1: 10059"
Figure legend: "B In Drp1 knockdown cells Bax-ring formation and cytochrome c release is not correlated. Three-color images of apoptotic mitochondria of Drp1 knockdown cells. From left to right: Tom22, Bax (recorded in the STED-mode), cytochrome c and an overlay of all three channels. The arrows point to Bax-rings. Scale bar: 1 µm."
NCBI gene IDs:
"Drp1: 10059"