EMBO/SourceData huggingface dataset:
Figure legend: "Primary adult microglia were cultivated in the presence of TGF-β (50 μg/ml) and M-CSF (10 ng/ml), while neonatal cells were stimulated for 24 hours with TGF-β (50 μg/ml) followed by 6 hours stimulation with LPS (1 ng/mL) or left untreated. Expression levels of microglia homeostatic (Olfml3, Tmem119, Gpr34) and inflammatory (Il1β, Tnf, Ccl2) genes were analysed by qPCR. Bars represent mean of relative expression (Gapdh as housekeeping gene) ± SEM (**p &lt; 0.01 by two-tailed Student's test; n=3)."
NCBI gene IDs:
"Gapdh: 
Ccl2: 20296
Gpr34: 23890
Il1β: 16176
Olfml3: 99543
Tmem119: 231633
Tnf: 21926"
Figure legend: "Expression of specific homeostatic (Tmem119, P2ry12, Siglech) and inflammatory (Ccl2, Gpr84) genes overlaid on the 2D-tSNE space. Bars represent log2 (Count+1)."
NCBI gene IDs:
"Ccl2: 20296
Gpr84: 80910
P2ry12: 70839
Siglech: 233274
Tmem119: 231633"
Figure legend: "Heatmap showing examples of specific genes mainly up-regulated in \"main LPS\" (Manf) or \"subset LPS\" (Ash1l) and down-regulated in \"main LPS\" (Mef2c) or \"subset LPS\" (Lamp1) overlaid on the 2D-tSNE space. Bars represent log2 (Count+1)."
NCBI gene IDs:
"Ash1l: 192195
Lamp1: 16783
Manf: 74840
Mef2c: 17260"
Figure legend: "Pseudotime dynamics of inflammatory (Ccl12, Ccl2, Gpr84) and homeostatic (Mef2c, P2ry12, Siglech) genes in dependence on inferred cell states."
NCBI gene IDs:
"Ccl12: 20293
Ccl2: 20296
Gpr84: 80910
Mef2c: 17260
P2ry12: 70839
Siglech: 233274"
Figure legend: "C-E KRAS and TP53 alterations in the cancer genome atlas (TCGA) pan-cancer MPM dataset (n = 86 patients). Shown are clinical and molecular data plot with alteration frequencies (C) and patients reclassified as KRAS- or TP53-altered (asterisks), copy number variation data summary (D), and segments of the KRAS and TP53 loci (E)."
NCBI gene IDs:
"KRAS: 3845
TP53: 7157"
Figure legend: "Molecular and clinical features of the cancer genome atlas (TCGA) pan-cancer MPM patients (n = 87) stratified by the presence of KRAS standalone (n = 10) and combined KRAS/TP53 (n = 7) alterations. Shown are unsupervised hierarchical clustering of n = 86 patients (gene expression data were not available for one patient) by 40 genes significantly overexpressed in KRAS/TP53-altered over KRAS-altered over KRAS/TP53-normal patients (A) and data summaries of mononucleotide change signatures (B), of indices of genomic instability and mutation burden (C), of clinical features"
NCBI gene IDs:
"KRAS: 3845
TP53: 7157"
Figure legend: "Molecular and clinical features of the cancer genome atlas (TCGA) pan-cancer MPM patients (n = 87) stratified by the presence of KRAS standalone (n = 10) and combined KRAS/TP53 (n = 7) alterations. Shown are unsupervised hierarchical clustering of n = 86 patients (gene expression data were not available for one patient) by 40 genes significantly overexpressed in KRAS/TP53-altered over KRAS-altered over KRAS/TP53-normal patients overall survival (F)."
NCBI gene IDs:
"KRAS: 3845
TP53: 7157"
Figure legend: "H Data summary of mutant allelic frequency of KRAS compared with NF2 and BAP1 in all mutated samples from (A-G)."
NCBI gene IDs:
"BAP1: 8314
KRAS: 3845
NF2: 4771"
Figure legend: "Pleural fluid cell pellets and supernatants from 10 patients (called CRCINA #) with pleural effusion and pleural tumor samples from 17 patients (called TR#) with MPM were subjected to digital droplet polymerase chain reaction (ddPCR) for the detection of mutant (MUT) copies of KRAS codon 12/13 (KRASG12/13) and KRAS codon 61 (KRASQ61), as well as copies of TP53 and TERT. Diagnoses were lung adenocarcinoma (LUAD; n = 4) and MPM (n = 23). The assays were designed for detection of down to 1:20000 copies using EKVX (KRASWTTP53G610T), A549 (KRASG12STP53WT), NCI-H460 (KRASQ61HTP53WT), NCI-H3122 (KRASWTTP53E285V), and NCI-H3255 (KRASWTTP53G560-1A) human LUAD cells as controls. Shown are individual patient (KRAS plot) and individual sample (TP53 plot) allelic frequencies with color code and limits of normal TP53 allelic frequency as vertical dashed lines in the TP53 plot (A) Any number of KRAS-mutant droplets detected in any sample (KRAS plot in A) and any patient that failed to achieve normal TP53 ploidy by any sample (TP53 plot in A) was deemed altered."
NCBI gene IDs:
"KRAS: 3845
TERT: 7015
TP53: 7157"
Figure legend: "F Representative May-Gruenwald-Giemsa-stained pleural fluid cytocentrifugal specimen from a KRASG12D;Trp53f/f mouse showing macrophages (MΦ, black arrow), lymphocytes (LΦ, purple arrow), and neutrophils (NΦ, green arrow) and summary of cellular and biochemical features of effusions of KRASG12D;Trp53f/f mice (n = 10)."
NCBI gene IDs:
"KRAS: 16653
Trp53f: 22059"
Figure legend: "Wild-type (Wt), KRASG12D, and Trp53f/f mice were intercrossed, and all possible offspring genotypes received 5 x 108 PFU intrapleural or intratracheal Ad-Cre and were sacrificed when moribund. In parallel, C57BL/6 mice received ten consecutive weekly intraperitoneal injections of 1 g/Kg urethane and were sacrificed after six months. Data summary (heatmap) and representative images of immunoreactivity of tissue sections of pleural and pulmonary tissues and tumors from these mice for different markers of human malignant pleural mesothelioma (MPM) and lung adenocarcinoma (LUAD). n = 10 mice/group were analyzed for each marker. Brown color indicates immunoreactivity and blue color nuclear hematoxylin counterstaining. Note the ubiquitous strong expression of Wilms' tumor 1 (WT1), patchy moderate expression of vimentin (VIM), ubiquitous moderate expression of mesothelin (MSLN), ubiquitous strong expression of calretinin (CALB2), podoplanin (PDPN), and osteopontin (SPP1), patchy moderate expression of cytokeratin 5/6 (CK5/6), and the absence of expression of surfactant protein C (SFTPC) in murine KRAS-driven mesotheliomas. Note also the ubiquitous strong expression of WT1, the patchy moderate expression of VIM, the ubiquitous low-level expression of MSLN, the ubiquitous strong expression of CALB2 and SPP1, the ubiquitous low-level expression of PDPN, the variable moderate expression of CK5/6, and the ubiquitous moderate expression of SFTPC in murine KRASG12D-driven and urethane-induced LUAD."
NCBI gene IDs:
"Cre: 2777477
KRAS: 16653
Trp53: 22059"
Figure legend: "E, F RT-PCR (E) and qPCR (F) of KPM cells and parental tumors show Trp53f/f allele deletion (Δ) and Bap1 and Cdkn2a overexpression compared with PMC."
NCBI gene IDs:
"Bap1: 104416
Cdkn2a: 12578
Trp53: 22059"
Figure legend: "I-K) Representaitve pictures showing that adult nerfin-1 clonal growth was significantly reduced on -his diet compared to CDD (measured at day 0, and day 9) quantified in (K) (n= 27, 110, 136). Scale bar = 75μm. ***p&lt;0.001 L-N) Representaitve pictures showing that larval nerfin-1 clonal growth was significantly reduced on 25% his diet compared to CDD (after 6 days), quantified in (N) (n=53,36). Scale bar = 50 μm. "
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "O-Q) Representaitve pictures showing that adult pros clonal growth was not significantly altered on -his diet compared to CDD (after 7 days), quantified in (Q) (n = 12, 23,16). Scale bar = 75μm. R-T) Representaitve pictures showing that larval pros clonal growth was not significantly altered by dietary histidine reduction (25% his) compared to CDD (after 6 days), quantified in (T) (n=19,13). Scale bar = 50 μm. "
NCBI gene IDs:
"pros: 41363"
Figure legend: "U-W) Representative pictures showing that the growth of larval type II lineages overexpressing NACT was significantly reduced dietary histidine reduction (25% his) compared to CDD, quantified in (W) (n=27,19). Scale bar = 100 μm."
NCBI gene IDs:
"NACT: 31293"
Figure legend: "C) Dietary supplementation of 3-methyl-L-his for 4 days significantly increased adult nerfin-1 clonal growth, and rescued nerfin-1 growth inhibition due to his dietary withdrawal (n =18,14, 7,9)."
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "D) Dietary supplementation of histamine does not significantly increase adult nerfin-1 clone size, but can rescue nerfin-1 clonal growth inhibition due to his dietary withdrawal (n =42,14,23,25)."
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "E) Larval nerfin-1 clonal growth is significantly decreased upon overexpression of HDC RNAi (n=23,36)."
NCBI gene IDs:
"HDC: 36076
nerfin-1: 44786"
Figure legend: "G) Hdc inhibition significantly reduced the percentage of Dpn+ NBs (n=16,17), and significantly increased the percentage of Elav+ neurons per clone (n=16,17)."
NCBI gene IDs:
"Hdc: 36076"
Figure legend: "H) HdcRi overexpression reduced cellular growth of nerfin-1 NBs (n=17,19) and neurons (n=36,19) measured as the ratio of nucleolus/nuclear volume."
NCBI gene IDs:
"Hdc: 36076
nerfin-1: 44786"
Figure legend: "A-E) Representative images of nerfin-1 clones in the adult VNC after 6 days of feeding on CDD or -his diet, scale bar = 100μm. Histidine dietary withdrawal resulted in clones significantly altering the amount of cell death per clone quantified in (C) (n = 6, 7)."
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "F-G) Larval nerfin-1 clones consisted of significantly greater proportion of differentiated neurons (n=10,7) and significantly reduced proportion of NBs per clone upon his dietary depletion (25% his, n=9,11)."
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "Representative images of adult nerfin-1 (I-J', green ) and pros clones (K-L', green), scale bar = 5μm. In nerfin-1 clones, the size of the NBs (Ase+, blue) was significantly reduced upon 6 days of histidine dietary withdrawal, quantified in (H) (n=33,20). In pros clones, the size of the NBs (Ase+ blue), and cellular growth measured as the ratio of nucleolus (Fib, white)/nuclear (Ase, blue) volume was not significantly altered upon 6 days of histidine dietary withdrawal, quantified in (H) (n=9,8)"
NCBI gene IDs:
"nerfin-1: 44786
pros: 41363"
Figure legend: "Cellular growth measured as the ratio of nucleolus /nuclear volume was significantly reduced in larval nerfin-1 NBs after 6 days his dietary reduction (25% his compared to CDD, n=14,6) quantified in (N)."
NCBI gene IDs:
"nerfin-1: 44786"
Figure legend: "In pros clones, the size of the NBs (Ase+ blue), and cellular growth measured as the ratio of nucleolus (Fib, white)/nuclear (Ase, blue) volume was not significantly altered upon 6 days of histidine dietary withdrawal, quantified in (O) (n=9,7)."
NCBI gene IDs:
"pros: 41363"
Figure legend: "Panel (P) shows that the larval nerfin-1 neuron to NB reversion requires 15-fold increase in cellular volume (n=10) and pros GMC to NB reversion requires approximately 3.5-fold increase in cellular volume (n=31)."
NCBI gene IDs:
"nerfin-1: 44786
pros: 41363"
Figure legend: "A-D) Representative pictures showing that pros NBs and GMCs are smaller than wildtype (NBs and GMCs are distinguished at telophase, as a doublet of unequal size), quantified in C (n=25,31,21,16). pros NBs exhibit similar nucleolus /nuclear ratio (white arrows, nucleolus marked by Fib) compared to wild type and pros;TorDN NBs, quantified in D (n=10,18,15)."
NCBI gene IDs:
"pros: 41363
Tor: 47396"
Figure legend: "E-F) Representative pictures showing pros clones (green) generated in myc hypomorphic background did not significantly alter pros nucleolus/nuclear ratio (E-F)"
NCBI gene IDs:
"myc: 31310
pros: 41363"
Figure legend: "pros clones (green) generated in myc hypomorphic background did not significantly alter pros nucleolus/nuclear ratio (E-F), nucleolus labelled with Fib (red) [quantified in G (n=44,43)], clone size [quantified in H (n=41,34)], or the proportion of Dpn+ NBs and Elav+ neurons per clone [quantified in I (n=15,15,15,15)]."
NCBI gene IDs:
"myc: 31310
pros: 41363"
Figure legend: "J-K) Representative pictures showing that nerfin-1 clones (green) generated in myc hypomorphic background (J-K)"
NCBI gene IDs:
"myc: 31310
nerfin-1: 44786"
Figure legend: "nerfin-1 clones (green) generated in myc hypomorphic background (J-K) exhibited significantly reduced nucleolus/nuclear ratio, [nucleolus labelled with Fib (red)] and reduced clone size, quantified in L (n=34,27) and M (n= 106,75), and displayed an increased proportion of differentiated Elav+ neurons, and reduced proportion of Dpn+ NBs per clone, quantified in N (n=20,20,20,20). scale bar =10μm"
NCBI gene IDs:
"myc: 31310
nerfin-1: 44786"
Figure legend: "O) Reducing Myc dosage with the hypomorphic Myc allele, mycP0, abolished the increase in nerfin-1 clone size mediated by 2x histidine dietary intake, 2x his (n=41,18,10,57)."
NCBI gene IDs:
"myc: 31310
Myc: 31310
nerfin-1: 44786"
Figure legend: "P) Overexpression of myc restored the growth of nerfin-1 clones inhibited by histidine dietary reduction, 25% his (n=51,36,39,29)."
NCBI gene IDs:
"myc: 31310
nerfin-1: 44786"
Figure legend: "Q, R) pros (Q) and nerfin-1 (R) clones are reduced upon Tor inhibition (n=22,63) compared to control (n=51,65), but their growth differentially depend on amino acid transporter Slif ( n=12,32) and downstream growth regulators eIF4E (n= 21,47) and S6K (n=39,37)."
NCBI gene IDs:
"eIF4E: 45525
nerfin-1: 44786
pros: 41363
S6K: 38654
Slif: 40510
Tor: 47396"
Figure legend: "(A) The mRNA level of ATG-5 was detected by real time PCR after treatment with siRNAs. GAPDH was used as internal controls."
NCBI gene IDs:
"ATG-5: 
GAPDH: 2597"
Figure legend: "C. MDCK cells stably expressing human CD63 were cultured sEVs were isolated from the pre-cleared medium by direct immunoaffinity capture using anti-CD63 antibody."
NCBI gene IDs:
"CD63: 967"
Figure legend: "F. MDCK cells stably expressing human CD63 were cultured sEVs were isolated from the pre-cleared medium by direct immunoaffinity capture using anti-CD63 antibody."
NCBI gene IDs:
"CD63: 967"
Figure legend: "B. Splicing of XBP1 mRNA was assessed by RT-PCR. Products from unspliced (XBP1u) and spliced (XBP1s) transcripts were resolved on a nondenaturing polyacrylamide gel and stained with SYBR green."
NCBI gene IDs:
"XBP1: 7494"
Figure legend: "C. Relative mRNA expression in proximal hind limb muscle (mean +/− SEM). Mice evaluated were littermate WT (n = 6), AR113Q (n = 6), castrated WT (C-WT, n = 6) and castrated AR113Q males (C-AR113Q, n = 5) on a mixed C57BL/6J-129 genetic background. *p&lt;0.05 by ANOVA."
NCBI gene IDs:
"AR: 11835"
Figure legend: "E. Relative mRNA expression in proximal hind limb muscle of AR21Q (n = 5) and AR113Q (n = 3) males backcrossed to C57BL/6J. ** p&lt;0.01, ***p&lt;0.001 by Student's t test. F. Relative mRNA expression in spinal cord of AR21Q (n = 5) and AR113Q (n = 3) males (mean +/− SEM). n. s. = not significant by Student's t test."
NCBI gene IDs:
"AR: 11835"
Figure legend: "A. Muscle fiber size (100 fibers/mouse) was quantified from proximal hind limb muscle of AR113Q (black) or AR113Q, CHOP −/− mice (white) at 12 wks. Left panel shows fiber size distribution, middle panel shows cumulative percent of fibers as a function of fiber area, and right panel shows relative fiber cross sectional area (mean +/− SEM). Left, middle panels, p&lt;0.0001 by Mann-Whitney test. Right panel, p&lt;0.001 by Student's t test."
NCBI gene IDs:
"AR: 11835
CHOP: 13198"
Figure legend: "C. Distribution of proximal hind limbmuscle fiber size from wt (black) and CHOP −/− (white) mice at 12 wks. Difference not significant by Mann-Whitney test."
NCBI gene IDs:
"CHOP: 13198"
Figure legend: "A. Relative expression of MurRF1 and MAFbx mRNAs in proximal hind limbmuscle of 12 wk mice (n = 5-6/genotype). *p&lt;0.05 by ANOVA, n. s. = not significant."
NCBI gene IDs:
"MAFbx: 67731
MurRF1: 433766"
Figure legend: "Denervated gastrocnemius muscles or contralateral intact controls were harvested at the indicated times following unilateral sciatic nerve transection in 6 wk male mice. A. Western blot shows enhanced eIF2 alpha phosphorylation (top) and RT-PCR demonstrates increased XBP1 mRNA splicing (bottom) in denervated muscle. Right panels show relative quantification of signal intensity. *p&lt;0.05 by Student's t test."
NCBI gene IDs:
"XBP1: 22433"
Figure legend: ". B. Relative expression of BiP, ATF4 and CHOP mRNA (n = 3). *p&lt;0.05 by Student's t test."
NCBI gene IDs:
"ATF4: 11911
CHOP: 13198
BiP: 14828"
Figure legend: "C. Following surgical denervation of wild type or CHOP−/− mice, LC3 and p62 expression was assessed by western blot. Right panels show quantification of signal relative to GAPDH. ***p&lt;0.001 by ANOVA, n. s. = not significant."
NCBI gene IDs:
"CHOP: 13198"
Figure legend: "D. Muscle fiber size (100 fibers/mouse) was quantified from wild type (black, n = 5), CHOP −/− (white, n = 3) and Beclin-1 +/− (grey, n = 3) mice 7 days post sciatic nerve transection. Shown is relative fiber cross sectional area (mean +/− SEM). ***p&lt;0.001 by ANOVA."
NCBI gene IDs:
"Beclin-1: 56208
CHOP: 13198"
Figure legend: "E. Muscle fiber size (100 fibers/mouse) was quantified from CHOP −/− (black, n = 6) or CHOP −/−, Beclin-1 +/− mice (white, n = 6) 7 days post sciatic nerve transection. Left panel shows fiber size distribution, middle panel shows cumulative percent of fibers as a function of fiber area, and right panel shows relative fiber cross sectional area (mean +/− SEM). Left, middle panels, p&lt;0.0001 by Mann-Whitney test. Right panel, p&lt;0.001 by Student's t test."
NCBI gene IDs:
"Beclin-1: 56208
CHOP: 13198"
Figure legend: "A. Muscle fiber size (100 fibers/mouse) was quantified from proximal hind limbmuscle of AR113Q (red, n = 6) or AR113Q, Beclin-1 +/− (blue, n = 6) mice at 16 wks. Left panel shows fiber size distribution, and right panel shows relative fiber cross sectional area (mean +/− SEM). Left panel, p&lt;0.0001 by Mann-Whitney test. Right panel, p&lt;0.0001 by Student's t test."
NCBI gene IDs:
"AR: 11835
Beclin-1: 56208"
Figure legend: "A. Left panel, Kaplan-Meyer survival curve of AR113Q males (red line, n = 12) and AR113Q, Beclin-1 +/− males (blue line, n = 15). *p&lt;0.05 by log-rank analysis. Right panel, mean survival +/− SEM. *p&lt;0.05 by Student's t-test."
NCBI gene IDs:
"AR: 11835
Beclin-1: 56208"
Figure legend: "B, C. Body weight (panel B) and grip strength (panel C) at different ages for wild type (wt, green line, n = 7), Beclin-1 +/− (yellow line n = 9), AR113Q (red line, n = 12), and AR113Q, Beclin-1 +/− (blue line, n = 15) male mice."
NCBI gene IDs:
"AR: 11835
Beclin-1: 56208"
Figure legend: "A. Western blot analysis of supernatants and whole cell lysates collected from WT and IQGAP1 KO YAMC cells with and without LPS stimulation for 4 hours or LPS plus ATP (4 hours plus 30 minutes) treatment as indicated."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "B. Nanoparticle tracking analysis of supernatant of WT and IQGAP1 KO YAMC cells treated with LPS plus ATP. **, P&lt;0.01 by unpaired two-tailed t test (n=3 biological repeats in one experiments)."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "D. Western blot analysis of enriched extracellular vesicles from supernatants WT and IQGAP1 KO YAMC cells."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "F. Flow cytometry analysis for CD63+ exosomes from supernatants of LPS plus ATP treated WT and IQGAP1 KO supernatants. Quantification was done by mean fluorescence intensity. **, P&lt;0.01 by unpaired two-tailed t test (n=4)."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "B. Co-immunoprecipitation of GSDMD with full-length and deletion mutants of IQGAP1 tested in HEK293 cells."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "C. Co-immunoprecipitation of GSDMD cleavage mutant D276A with IQGAP1 overexpressed in HEK293 cells."
NCBI gene IDs:
"GSDMD: 69146
IQGAP1: 29875"
Figure legend: "D. Co-immunoprecipitation of GSDMD with IQGAP1 in WT and cleavage mutant (D276A) restored GSDMD KO YAMC cells. Cells were treated as indicated."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "E. Co-immunoprecipitation of C-terminal GSDMD (CT) with IQGAP1 overexpressed in HEK293 cells."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "A. Imaging analysis for co-localization of CD63 with IQGAP1 in IQGAP1 (FL) and IQ domain truncation (ΔIQ) restored YAMC cell. Cells were treated with LPS plus ATP. The IQGAP1-CD63 co-localization dots (shown by yellow) were counted, and Pearson's correlation coefficient was calculated for the quantification of co-localization."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "B. Imaging analysis for co-localization of CD63 with GSDMD in IQGAP1 full length (FL) and IQ domain truncation restored YAMC cell (ΔIQ) after LPS plus ATP treatment. The GSDMD-CD63 co-localized dots were counted and Pearson's correlation coefficient was calculated for quantification."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "C. Proximity ligation assay for IQGAP1 and GSDMD in IQGAP1 FL restored cells and ΔIQ restored cells."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "D. Western blots analysis for supernatants collected from IQGAP1 KO YAMC cells, IQGAP1 full length and IQ domain truncation restored IQGAP1 KO YAMC cells. Cells were treated as indicated and the supernatants were subjected to IP with anti-IL-1β. E. Western blots analysis for whole cell lysate collected from panel D."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "A. Imaging analysis for early endosome (shown by marker EEA1) change in WT and IQGAP1 KO YAMC cells upon indicated treatments. Quantification was done by counting the EEA1 puncta from each cell. Five field views of cells' puncta were counted. Three independent experiments were repeated"
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "B. Co-localization of CD63 and IQGAP1 in YAMC and GSDMD KO YAMC cells. Yellow dots indicated the co-localization signal."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "C. Co-localization of CD63 and GSDMD in YAMC and IQGAP1 KO YAMC cells."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "E. Co-localization of CD63 and pro-IL-1β in YAMC and IQGAP1 KO YAMC cells."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "F. Western blot analysis of extracellular vesicle secretion from WT and TSG101 knockdown cells upon indicated treatments. Secreted proteins were pulled down by IL-1β in the supernatant and the indicated proteins were probed for."
NCBI gene IDs:
"TSG101: 22088"
Figure legend: "G. Total particle count and size distribution for exosomes collected from WT and TSG101 knockdown cell supernatants with indicated treatment."
NCBI gene IDs:
"TSG101: 22088"
Figure legend: "H. Western blot analysis for interaction of TSG101 with IQGAP1 in WT and GSDMD KO cells by pulling down IQGAP1 under indicated treatments."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "I. Co-immunoprecipitation of GSDMD with TSG101 in WT and IQGAP1 KO cells by pulling down TSG101 under indicated treatments."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "A. Total particle count and size distribution of exosomes secreted in the supernatant from IQGAP1 KO (IQ1 KO), IQGAP1 full length restored (FL) and GRD domain truncation restored (ΔGRD) YAMC cells upon LPS or LPS plus ATP treatment."
NCBI gene IDs:
"IQ1: 29875
IQGAP1: 29875"
Figure legend: "B. PLA assay for IQGAP1 and CDC42 interaction in IQGAP1 FL restored cell and ΔGRD restored cell. The green dots showed the interaction of IQGAP1 and CDC42."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "C. Colocalization of CD63 with IQGAP1-CDC42 complex (shown by PLA signal) in WT and GSDMD KO cells under LPS treatment. Quantification was performed by counting PLA green dots."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "D. Co-immunoprecipitation analysis of endogenous IQGAP1 with CDC42 in wild-type and GSDMD KO cells upon LPS or LPS plus ATP treatment."
NCBI gene IDs:
"GSDMD: 69146"
Figure legend: "F. Co-immunoprecipitation analysis of endogenous GSDMD with CDC42 in wild-type and IQGAP1 KO cells upon LPS or LPS plus ATP treatment."
NCBI gene IDs:
"IQGAP1: 29875"
Figure legend: "B. Total particle count and size distribution for exosomes collected from colon explant of DSS treated IQGAP1 or GSDMD knockout mice and their respective controls."
NCBI gene IDs:
"GSDMD: 69146
IQGAP1: 29875"
Figure legend: "C. Western blot analysis for supernatant collected from colon explants of DSS-treated wild-type (WT), Gsdmd-/-(GSDMD KO) and Iqgap1-/- (IQGAP1 KO) mice. Supernatant was first pulled down by IL-1β antibody and samples were subjected to Western blot analysis. The indicated proteins were probed."
NCBI gene IDs:
"Gsdmd: 69146
Iqgap1: 29875"