EMBO/SourceData huggingface dataset:
Figure legend: "C In-gel kinase assays of OST1 in Col-0 and ost1-3 mutant under cold stress. Twelve-day-old Col-0 and ost1-3 mutant were treated at 4°C for 2 h. Total protein extracts were prepared and separated on a SDS-PAGE gel containing 0.2 mg/mL GST-AtANN1 as a substrate, and incubated with 70 µCi [γ-32P] ATP. Top, autoradiograph; bottom, CBB staining."
NCBI gene IDs:
"ost1: 829541"
Figure legend: "E OST1 phosphorylates AtANN1 at S289 in vivo in LC/MS analysis. Total proteins were extracted from 12-d-old AtANN1-Myc overexpressing plants treated at 4°C for 0, 10, 30 and 120 min, followed by trypsin digestion. Phosphopeptides were enriched for mass spectrometry analysis. Representative picture for 10 min was shown."
NCBI gene IDs:
"Myc: 
AtANN1: 840476"
Figure legend: "Freezing phenotype (A) of Col-0, atann1, and atann1 AtANN1S289E (#5) plants. 12-d-old plants grown on MS medium at 22°C were exposed to freezing temperatures (NA, −5°C, 30 min; CA, −8°C, 40 min). Representative pictures are shown in (A)."
NCBI gene IDs:
"atann1: 840476
AtANN1: 840476"
Figure legend: "survival rate (B), and ion leakage (C) of Col-0, atann1, and atann1 AtANN1S289E (#5) plants. 12-d-old plants grown on MS medium at 22°C were exposed to freezing temperatures (NA, −5°C, 30 min; CA, −8°C, 40 min)."
NCBI gene IDs:
"atann1: 840476
AtANN1: 840476"
Figure legend: "Freezing phenotype (D) of Col-0, Atann1, and atann1 AtANN1S289A (#16) plants. 12-d-old plants grown on MS medium at 22°C were exposed to freezing temperatures (NA, −5°C, 30 min; CA, −8°C, 40 min). Representative pictures are shown in (D)."
NCBI gene IDs:
"Atann1: 840476
atann1: 840476
AtANN1: 840476"
Figure legend: "survival rate (E), and ion leakage (F) of Col-0, Atann1, and atann1 AtANN1S289A (#16) plants. 12-d-old plants grown on MS medium at 22°C were exposed to freezing temperatures (NA, −5°C, 30 min; CA, −8°C, 40 min)."
NCBI gene IDs:
"Atann1: 840476
atann1: 840476
AtANN1: 840476"
Figure legend: "A AtANN1 current recordings in Xenopus oocytes. Whole-cell currents were recorded in Xenopus oocytes injected with ddH2O or with AtANN1, OST1, or AtANN1 + OST1 cRNA. Bath solution was described in Materials and Methods. The voltage protocol as well as time and current scale bars for the recordings are shown. B Current-voltage (I-V) relationship of the steady-state whole-cell currents in Xenopus oocytes described in (A). Negative current is influx of cations into the oocytes. "
NCBI gene IDs:
"AtANN1: 840476
OST1: 829541"
Figure legend: "D AtANN1 current recordings in oocytes. Whole-cell currents were recorded in Xenopus oocytes injected with ddH2O or with AtANN1, AtANN1S289A, OST1, AtANN1 + OST1, and AtANN1S289A + OST1 cRNA. E Current-voltage (I-V) relationship of the steady-state whole-cell currents in Xenopus oocytes described in (D). "
NCBI gene IDs:
"AtANN1: 840476
OST1: 829541"
Figure legend: "A Time-course analysis of [Ca2+]cyt dynamics between 10-day-old wild type (Col-0) and ost1-3 mutant after treatment with ice-cold water or ice-cold water containing 10 mM LaCl3 (arrow shows the time point of treatment). Luminescence was recorded at 1-s intervals. B Quantification of the cold-induced [Ca2+]cyt changes shown in (A). Peak [Ca2+]cyt indicates the highest [Ca2+]cyt after treatment. "
NCBI gene IDs:
"ost1: 829541"
Figure legend: "Micrographs of maximum intensity projections of forebrain sections from 3 mo old GLASTCreERT2/eGFP mice  21 days after tamoxifen treatment Double-positive cells are indicated by arrowheads. CTX: cerebral cortex; DIE: diencephalon; HC: hippocampus. Scale bars: 300 µm (A), 20 µm (B), 75 µm (C), 30 µm (D), Histograms showing quantifications (B, n = 6 animals; 5 region of interest (ROI) covering the diencephalon on a total of 3 slides analyzed per animal Data information: data are presented as mean ± SEM. Each data point represents one animal."
NCBI gene IDs:
"eGFP: 
Cre: 2777477
ERT2: 26417
GLAST: 20512"
Figure legend: "A-E Micrographs of maximum intensity projections (A - D) or single optical section (E) of forebrain sections stained  as indicated for fluorescent proteins in astrocytes labelled in 8 mo (A, B, E) and 3 mo (C, D) GLASTCreERT2/Confetti mice  at 21 dpt. Note that clusters of duplets (D) and triplets (C) labelled in the same color can be found only in the DIE. Also note that astrocyte somata are often very close together, as also observed after live in vivo imaging of dividing astrocytes Somata  of single cells are highlighted in circles. Data information: CTX: cerebral cortex; DIE: diencephalon; HC: hippocampus. Scale bars: 300 µm (A), 100 µm (B), 50 µm (C, D), 20 µm (E)."
NCBI gene IDs:
"Confetti: 
Cre: 2777477
ERT2: 26417
GLAST: 20512"
Figure legend: "B Micrographs of forebrain  sections of 3 mo old GLASTCreERT2/RPL22HA/eGFP 21 dpt mice stained for HA  show maximum intensity projections of HA+ expressing cells with  astroglial morphology. CTX GM: cerebral cortex grey matter; DIE: diencephalon. Scale bars: 100 µm. C Micrographs of forebrain sections of 3 mo old GLASTCreERT2/RPL22HA/eGFP mice 21dpt  stained  for HA and S100β  showing the specificity of the mouse line with HA  detected only in S100β+ astrocytes. DIE: diencephalon. Scale bar: 70 μm. Data information: CTX GM: cerebral cortex grey matter; DIE: diencephalon; n=3 biological replicates, tissue from 3 mice was pooled"
NCBI gene IDs:
"eGFP: 
HA: 
Cre: 2777477
ERT2: 26417
RPL22: 19934
GLAST: 20512"
Figure legend: "C Example of a Smad4/GFP double-positive astrocyte (indicated by arrowhead) in a single optical section of DIE  from 3 mo old GLASTCreERT2/eGFP mice 21 dpt immunostained  as indicated on top of the panel. Orthogonal projections of the area highlighted in left panel of C shown at higher magnification in the right panel. Scale bar: 15 µm."
NCBI gene IDs:
"eGFP: 
Cre: 2777477
ERT2: 26417
GLAST: 20512"
Figure legend: "D Example of a proliferating  (PCNA+, arrowhead) astrocyte in a single optical section of DIE  from 3 mo old  GLASTCreERT2/eGFP mice  21 dpt immunostained as indicated on top with single channels shown in middle and right panels. Scale bars: 15 µm."
NCBI gene IDs:
"eGFP: 
Cre: 2777477
ERT2: 26417
GLAST: 20512"
Figure legend: "F, G Histograms depicting percentage of the  neurosphere-generating cells obtained from the DIE (F) or SEZ (G) of GLASTCreERT2/Smad4fl/fl/eGFP or GLASTCreERT2/Smad4WT/WT/eGFP, as indicated on the x-axis (n = 3-4 animals for SEZ, 4 to 8 technical replicates analyzed per animal; 4 animals for DIE, 8 technical replicates analyzed per animal). Data information: data are presented as mean ± SEM. F:  GFP  negative and GFP  positive cells comparing wt/wt vs fl/fl animals, adjusted P-value = 0.0408; G: GFP negative and GFP positive cells comparing wt/wt vs fl/fl animals, ns). Each dot represents one n. * P &lt; 0.05, ** P &lt; 0.01 nonparametric 2way ANOVA followed by Sidak's multiple comparisons test"
NCBI gene IDs:
"eGFP: 
Cre: 2777477
ERT2: 26417
GLAST: 20512
Smad4: 17128"
Figure legend: "H Histogram depicting the number  of secondary neurospheres  generated from 10000 Smad4f/f neurosphere cells at 7 days after infection with MLV-based retrovirus containing either CAG-NLS-Cre or only CAG-GFP (n = 11 cultures / animals, 3 technical replicates per condition analyzed; control vs. Cre, P-value = 0.0149). Note the decrease in  DIE astrocyte proliferation in vivo and in  neurosphere formation upon Smad4  deletion Data information: data are presented as mean ± SEM. Each dot represents one n. * P &lt; 0.05, ** P &lt; 0.01 nonparametric Mann-Whitney test)."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Smad4: 17128"
Figure legend: "A-C) Heads of E18.5 foetuses were serially sectioned in coronal orientation and stained with H&amp;E. Representative images of rostro-occipital level-matched sections from age-matched wild-type (n = 2), Bok-/-Bak-/-Bax-/- triple knockout (TKO; n = 4) and QKO foetuses (n = 4) depicting the forebrain, nasal cavity, and tongue are shown. BP, bony palate; Cx, cerebral cortex; ES, epidural space; In, incisor; LV; lateral ventricle; Ma, mandible; MC, mouth cavity; NC, nasal cavity; NS, nasal septum; St, striatum; SVZ, subventricular zone; To, tongue; WP, whisker follicles. Scale bar = 850 µm (A), 1200 µm (B, C)."
NCBI gene IDs:
"Bak: 12018
Bax: 12028
Bok: 51800"
Figure legend: "A-C) Representative H&amp;E-stained coronal serial sections of the eye from E18.5 wild-type (n = 2), Bok-/-Bak-/-Bax-/- triple knockout (n = 3), and Bok-/-Bak-/-Bax-/-Bid-/- quadruple knockout (n = 3) foetuses."
NCBI gene IDs:
"Bak: 12018
Bax: 12028
Bid: 12122
Bok: 51800"
Figure legend: "(A) Estimation of BID protein levels in TKO MEFs. DKO MEFs re-expressing human BAK, TKO MEFs, and two human leukaemia cell lines RS4;11 and MV4;11, were lysed and analysed by Western blot for BID levels, using a rat monoclonal antibody raised against human BID (anti-hBID clone 2D1) that also recognises mouse BID. Also present are samples of the human FL-BID protein added at the equivalent concentrations used in (B) and (C). On the right gel, duplicate samples of DKO and TKO cells and permeabilised DKO and TKO cells (from B and C) showing that endogenous mouse BID is cytosolic in these cells. Data shown are representative of 2 independent experiments."
NCBI gene IDs:
"BAK: 578"
Figure legend: "(B, C) Partial cytochrome c release by FL-BID from mitochondria of TKO MEFs when combined with BIM BH3 peptide or cBID. DKO MEFs re-expressing human BAK or TKO MEFs were permeabilised, and then the cells incubated with the indicated concentrations of Bim BH3 peptide, cBID or FL-BID, alone or in the indicated combinations, at 30oC for 30 min. Cells were then co-stained for cytochrome c and TOM20 and analysed by flow cytometry. Left hand panels show median fluorescence data from replicate experiments (squares denote experiment 1, circles denote experiment 2). Right hand panels show histograms of cytochrome c staining and TOM20 staining from experiment 2 (treatments are as listed in the left-hand panels)."
NCBI gene IDs:
"BAK: 578"
Figure legend: "B. Detection of netrin-1 expression  in the MMTV/neuT  model by immunoblots (Tumor  1 to 3)."
NCBI gene IDs:
"neuT: 24337"
Figure legend: "E. Biodistribution properties of NP137-NODAGA-111In  in MMTV/neuT mice  at 96 h (3 mice) and measured for all organs. Radioactivity  incorporation was quantified by the percentage of the injected dose per gram of organ. Error bars indicate s.d."
NCBI gene IDs:
"neuT: 24337"
Figure legend: "F. NP137-177Lu  enhances MMTV/neuT mouse survival. Mice  were treated at 3.5 months with PBS, DOTA-NP137  or NP137-177Lu. Kaplan-Meier survival curves  of mice  treated or not with NP137  and considered as dead when cumulative tumors volume reached 2,000 mm3. Mantel Cox test; n = 10 animals/group for PBS; n = 14 animals/group for DOTA-NP137; n = 8 animals/group for NP137-177Lu; p &lt; 0.001 between PBS  and NP137-177Lu  and between DOTA-NP137  and NP137-177Lu."
NCBI gene IDs:
"neuT: 24337"
Figure legend: "(A-C) qPCR  and western blot analysis indicated an upregulation of hepatic ZBTB22  in diabetic db/db mice, obese ob/ob mice  and high-fat diet  for 12 weeks mice  (n=6 mice). Data information: Data are means ± SEM. **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D) qPCR  and western blot analysis showed an increase of hepatic ZBTB22  in normal C57 mice  after overnight fasting, and a recovery of ZBTB22  expression after refeeding  (n=6 mice). Data information: Data are means ± SEM. **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(E) qPCR  and immunofluorescence  analysis displayed an enhanced expression of ZBTB22  in MPHs  after FSK  treatment for 24h and a reduction of ZBTB22  in MPHs  after insulin  treatment for 15min (n=4 biological replicates), scale bars represent 10 μm. Data information: Data are means ± SEM. **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(A-B) qPCR  analysis the change of genes involved in gluconeogenesis, lipogenesis and fatty acid oxidation in primary hepatocytes  after ZBTB22  overexpression (n=4 biological replicates). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D-E) ZBTB22  overexpression increase lipid  deposition in primary hepatocytes, as shown by the enhanced lipid TOX probes and TG  content (n=4 biological replicates), scale bars represent 10 μm. Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(F-G) qPCR  analysis the change of genes involved in gluconeogenesis, lipogenesis, and fatty acid oxidation in primary hepatocytes  after ZBTB22  knockdown (n=4 biological replicates). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(I-J) ZBTB22  knockdown suppress lipid  deposition in  primary hepatocytes, as shown by the weakened lipid TOX probes and TG  content (n=4 biological replicates), scale bars represent 10 μm. Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(K) qPCR  analysis of mRNA levels of Pgc1α, PCK1  in  primary hepatocytes infected with Ad-shCtrl or Ad-shZBTB22 with or without forskolin  treatment (n=4 biological replicates). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"PCK1: 18534
Pgc1α: 19017
ZBTB22: 81630"
Figure legend: "ZBTB22  deletion effectively decrease the  fat mass  of mice  fed on HFD (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D) ZBTB22  deletion enhanced the expression  of genes involved in thermogenesis and fatty acid oxidation in brown adipose tissue from mice  fed on HFD (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(E) ZBTB22  deletion increased the  heat production, CO2  generation and O2  consumption in mice  fed on HFD (n=3 mice)."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "ZBTB22  deletion effectively decrease the fasting blood glucose  of mice  fed on HFD (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D) ZBTB22  deletion significantly changed hepatic expression  of genes involved in gluconeogenesis, lipogenesis and fatty acid oxidation in mice  fed on HFD (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(E) ZBTB22  deletion significantly improved the phosphorylation of AKT  and GSK3β  in the liver  of mice  fed on HFD."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(I) Hepatic ZBTB22   overexpression induces glucose   intolerance  and  insulin  resistance in ZBTB22  deficiency mice  fed on HFD (n=6 mice)."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(J) Hepatic ZBTB22  overexpression decreased the insulin-induced phosphorylation  of AKT  and GSK3β  in the liver  of ZBTB22  deficiency mice  fed on HFD."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(K) qPCR  analysis of hepatic  genes involved in gluconeogenesis, lipogenesis and fatty acid oxidation in ZBTB22  deficiency mice  fed on HFD (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(A-B) Hepatic ZBTB22  silencing decreases the expression  of gene involved in gluconeogenesis, lipogenesis, and fatty acid oxidation in db/db mice  (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D) Hepatic ZBTB22  silencing improved the  glucose  tolerance,  insulin  sensitivity  and pyruvate  tolerance in db/db mice  (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(J-K) Hepatic ZBTB22  silencing decreases serum  inflammatory cytokines levels and hepatic  inflammatory genes expression (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "Hepatic ZBTB22   overexpression increases the expression  of genes involved in gluconeogenesis in normal C57 mice  (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(C) Hepatic ZBTB22  overexpression significantly induced glucose intolerance and   insulin resistance in normal C57 mice  (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "(D) Hepatic ZBTB22  overexpression significantly decreased insulin-induced phosphorylation  of hepatic AKT  and GSK3β  in normal C57 mice."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "Hepatic ZBTB22  overexpression increase serum  inflammatory cytokines levels Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "Hepatic ZBTB22  overexpression increase hepatic expression  of inflammatory genes (n=6 mice). Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "Western Blot analysis showed that ZBTB22-mediated induced the up-regulated expression of PEPCK1  in Ad-ZBTB22  infected mice primary hepatocytes  and livers  (C)"
NCBI gene IDs:
"ZBTB22: 81630"
Figure legend: "Chip  and Chip-qPCR  displayed increase occupancy of ZBTB22  on the promoter region of PCK1  (n=3 biological replicates)."
NCBI gene IDs:
"PCK1: 18534"
Figure legend: "PCK1  silencing , as well as the stimulatory effects of ZBTB22  on SREBP1C expression  (J) (n=4 biological replicates) and cellular glucose  output (K) (n=4 biological replicates) Data information: Data are means ± SEM. *p&lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"PCK1: 18534
SREBP1C: 20787
ZBTB22: 81630"
Figure legend: "Hepatic ZBTB22  overexpression failed to change  glucose tolerance,  insulin sensitivity  in PCK1-silencing C57 mice  (n=6 mice). Data information: Data are means ± SEM. *p &lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"PCK1: 18534
ZBTB22: 81630"
Figure legend: "(G) Hepatic ZBTB22  overexpression failed to change serum  and hepatic TG  and TC  levels in PCK1-silencing C57 mice  (n=6 mice). Data information: Data are means ± SEM. *p &lt; 0.05, **p&lt; 0.01, ***p&lt; 0.001 by Student's t test."
NCBI gene IDs:
"PCK1: 18534
ZBTB22: 81630"
Figure legend: "(b, c) Stable FoxO1 knockdown HCT116 cells, or cells with an empty RNAi plasmid, were incubated in a serum-free medium for up to 36 h (b) or treated with 0.5 mM H2O2 for up to 12 h (c); western blotting was performed to detect changes in FoxO1, p62, LC3-II or β-actin."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(e) Autophagic vesicles or autophagosomes in HCT116 cells or stable FoxO1-RNAi HCT116 cells, were observed by electron microscopy after 24 h of serum starvation. The bottom picture enlargement showing clearer autophagic vesicles indicated by an arrow. The graph shows a statistical analysis of autophagic vesicles in HCT116 cells with or without knockdown of FoxO1 in response to serum starvation. Data in e are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(f) LC3-immunopositive punctate signals were observed in HCT116 or in stable FoxO1-RNAi HCT116 cells in response to serum starvation for 24 h or 0.5 mM H2O2 treatment. (g) Quantification of LC3-positive punctate cells in f. Data in g are means ± s. d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(h) A rescue experiment using a specific mutated FoxO1 plasmid to validate the activity of FoxO1 in the induction of autophagy in HCT116 cells in the presence or absence of serum, with or without E64 and pepstatin A."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(a) A Flag-tagged FoxO1(WT), FoxO1(ΔDB), FoxO1(ΔDB3A) or an empty plasmid was individually transfected into GFP-LC3 pre-transfected H1299 cells, and the cellular localization of FoxO1 (upper panels) or GFP-LC3 punctate signals (lower panels) was observed with a confocal microscope. Scale bars, 10 μm. (b) The numbers of punctate GFP-LC3 cells are represented by the ratio of GFP-LC3-labelled punctate cells to overall survived H1299 cells (at least 200 cells were counted). Data in b are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308
LC3: 440738///81631///84557"
Figure legend: "(d) H1299 cells were labelled with [3H]leucine for 48 h, then transfected with a control plasmid or various Flag-FoxO1 plasmids, in the presence or absence of 3-MA or E64 and pepstatin A. Degradation of long-lived proteins was measured at the indicated time, 24 h after transfection. Data in d are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(e) A GFP-tagged FoxO1(3A) or an empty plasmid was co-transfected with a FoxO1-promoter luciferase plasmid into H1299 cells. After 24 h, luciferase reporter activity of FoxO1 was measured. RLU, relative luciferase units. Data are means ± s.d. (n = 3)."
NCBI gene IDs:
"luciferase: 
FoxO1: 2308"
Figure legend: "(f) A set of RNAi-resistant rescue forms of FoxO1 plasmids were transfected into stable FoxO1-RNAi HCT116 cells in the presence or absence of E64 and pepstatin A. Western blotting was performed to detect p62 degradation and LC3-II accumulation."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(g) Flag-tagged FoxO1(WT)r, FoxO1(3A)r, FoxO1(3A-NLSm)r, FoxO1(NESm)r or an empty plasmid were individually transfected into GFP-LC3 pre-transfected HCT116 cells (FoxO1-RNAi treated), and the cellular localization of FoxO1 (upper panels) or GFP-LC3 punctate signals (lower panels) were observed with a confocal microscope. (h) The numbers of punctate GFP-LC3 cells are represented by the ratio of GFP-LC3-labelled punctate cells to overall survived HCT116 cells. Data in h are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308
LC3: 440738///81631///84557"
Figure legend: "(a) Different FoxO1-expressing plasmids, namely FoxO1(WT), FoxO1(3A), FoxO1(ΔDB), FoxO1(ΔDB3A), FoxO1(NESm) and FoxO1(3A-NLSm), were transfected into H1299 cells and cell fractionation was performed to analyse cellular localization (N, nucleus; C, cytoplasm) of these FoxO1 mutants. PCAF is a loading control for nuclear protein, and actin is the loading control for cytoplasmic protein."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(b) Quantitative PCR was performed to measure the transactivity of autophagy-related genes in transfected H1299 cells with different FoxO1-expressing plasmids (upper panel) or in stable FoxO1-RNAi or non-RNAi-treated HCT116 cells in response to serum starvation for 24 h or 0.5 mM H2O2 for 12 h (lower panel). All data are means ± s.d. (n = 3)."
NCBI gene IDs:
"FoxO1: 2308"
Figure legend: "(c) Different FoxO1-mutant-expressing plasmids were transfected into H1299 cells and a ChIP assay was performed to measure the binding activity of these mutated FoxO1 to the p27 promoter."
NCBI gene IDs:
"p27: 1027
FoxO1: 2308"
Figure legend: "(b) HCT116 cells were serum-starved for 24 h and the cytosolic proteins were extracted for co-immunoprecipitation with anti-FoxO1 or anti-SIRT2, followed by probing with anti-SIRT2 or anti-FoxO1, respectively (top and middle panels). FoxO1(WT) was also transfected into H1299 cells and the cytosolic protein was extracted, followed by co-immunoprecipitation with anti-FoxO1 and probing with anti-SIRT2 (bottom panels)."
NCBI gene IDs:
"FoxO1: 2308
SIRT2: 22933"