EMBO/SourceData huggingface dataset:
Figure legend: "(C) Tumor volumes of untreated (control) and sunitinib-treated NeuT;Apln+/+ and NeuT;Apln-/- mammary tumors, size-matched at 20-70mm3 and followed over time by MRI analysis; NeuT;Apln+/+ Control (n=7), NeuT;Apln-/- Control (n=5), NeuT;Apln+/+ Sunitinib (n=5), NeuT;Apln-/- Sunitinib (n=6) mice per group; Lines represent nonlinear fit of tumor growth. Box and arrow indicate the time point used for analysis in panel D. (D) Mitotic counts (mean ± S.E.M.) and representative H&amp;E images of mammary tumors in untreated (control) ­and sunitinib-treated NeuT;Apln+/+ and NeuT;Apln-/- mice, assessed 6 weeks after tumor onset.; NeuT;Apln+/+ Control (n=7), NeuT;Apln-/- Control (n=6), NeuT;Apln+/+ Sunitinib (n=9), NeuT;Apln-/- Sunitinib (n=4) tumors per group; *P&lt;0.05; one-way ANOVA to sunitinib-treated NeuT;Apln-/-. White arrows indicate mitotic figures. Scale bars = 50 μm"
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(B) Representative MRI images; Scale bar = 5mm and (C) quantification (mean ± S.E.M.) of relative tumor blood volume (rTBV) over time after NeuT+ mammary tumors were size-matched at 20-70mm3 (0 weeks). Treatments and genotypes are indicated. NeuT;Apln+/+ Control (n=4), NeuT;Apln-/- Control (n=4), NeuT;Apln+/+ Sunitinib (n=5), NeuT;Apln-/- Sunitinib (n=5); **P&lt;0.01, and ***P&lt;0.001 compared to untreated NeuT;Apln+/+ mice and #P&lt;0.05 compared to untreated control NeuT;Apln-/- mice; two-way ANOVA. Of note, in NeuT;Apln+/+ mice, only 2 tumors could be analyzed after 6 weeks as some mice had to be sacrificed due to the large tumor sizes following ethical guidelines. Thus, we did not perform any statistical analysis on the 6 weeks timepoints."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(D) Analysis (mean values ± S.E.M.) of CD31+ area (x104 μm2)/field, number of dilated tumor vessels and percentage of alphaSMA+ area per CD31+ blood vessels in mammary tumors of untreated (control) and sunitinib-treated NeuT;Apln+/+ and NeuT;Apln-/- mice, assessed 6 weeks after mammary tumors were size-matched. NeuT;Apln+/+ Control (n=4), NeuT;Apln-/- Control (n=4), NeuT;Apln+/+ Sunitinib (n=5), NeuT;Apln-/- Sunitinib (n=4); *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; one-way ANOVA and Kruskal-Wallis test. (E) Representative immunofluorescence and immunohistochemistry images from Fig. 5D quantification. Dilated blood vessels are marked by a red asterisk. DAPI (blue) is shown as a counterstain to visualize nuclei. Scale bars = 100 μm (upper panels), 50 μm (middel panels) and 20 μm (lower panels)."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(A) Percentages of CA9+ cells adjacent to CD31+ tumor blood vessels in untreated (control) and sunitinib-treated NeuT;Apln+/+ and NeuT;Apln-/- mice, 6 weeks after mammary tumors were size-matched. NeuT;Apln+/+ Control (n=4), NeuT;Apln-/- Control (n=4), NeuT;Apln+/+ Sunitinib (n=4), NeuT;Apln-/- Sunitinib (n=4); 100-200 peri-vascular intra-tumoral regions per group were counted. ***P&lt;0.001; one-way ANOVA. Right panels show representative immunofluorescent images. Areas limited by dotted white lines indicate CA9+ areas. Scale bars = 50 μm."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(B) Representative MRI images; Scale bar = 5mm and (C) quantification (mean ± S.E.M.) of vessel permeability (K2) in NeuT+ mammary tumors followed over time. Treatments and genotypes are indicated. NeuT;Apln+/+ Control (n=4), NeuT;Apln-/- Control (n=5), NeuT;Apln+/+ Sunitinib (n=4), NeuT;Apln-/- Sunitinib (n=5); ***P&lt;0.001, compared to untreated control NeuT;Apln+/+ mice and §P&lt;0.05 compared to sunitinib-treated NeuT;Apln+/+ mice; two-way ANOVA. Of note, in NeuT;Apln+/+ mice, only 2 tumors could be analysed after 6 weeks as some mice had to be sacrificed due to the large tumor sizes following ethical guidelines. Thus we did not perform any statistical analysis on the 6 weeks timepoints."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(A) Number of metastatic lung foci in untreated (control) and sunitinib-treated (60mg/kg, three times a week from tumor initiation) NeuT;Apln+/+ or NeuT;Apln-/- mice, assessed 6 weeks after mammary tumors were size-matched. Data of individual lung sections and means (black bars) are shown. Right panels show representative H&amp;E images, where black arrows and insets indicate metastatic foci. Scale bars = 1000 μm (large panels) and 50 μm (insets). *P&lt;0.05, **P&lt;0.01; ***P&lt;0.001; Kruskal-Wallis test; n=3 mice per cohort and three sections per lung were analysed."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "A AP and SAP were induced in wild type (WT) and ANGPTL4 -/- mice (n = 15, each group), as described in the material and methods. H&amp;E staining, TUNEL staining for apoptosis, and immunofluorescence staining for ANGPTL4 (red) and amylase (green) were performed in the pancreatic tissues. ANGPTL4 and IL-6 protein expressions were also determined in pancreatic tissues from ANGPTL4 WT and -/- mice by Western blotting. Scale bar represents 100 µm."
NCBI gene IDs:
"ANGPTL4: 57875"
Figure legend: "C Immunofluorescence staining of TNF-α and IL-6, and the levels of the inflammatory cytokines TGF-β, IFN-γ, and IL-1β in the sera from ANGPTL4 WT and -/- mice (n = 10, each group). Scale bar represents 150 µm"
NCBI gene IDs:
"ANGPTL4: 57875"
Figure legend: "C Immunofluorescent staining of the F4/80 macrophage marker and quantification of macrophage-positive cells in the AP and SAP groups from the ANGPTL4 -/-and WT mice (n = 10). Scale bar represents 150 µm"
NCBI gene IDs:
"ANGPTL4: 57875"
Figure legend: "A Cell morphology and the mRNA levels of inflammatory cytokines, including and TNF-α, IL-6, and IL-1β in mouse primary macrophages treated with LPS and ANGPTL4 (100 ng/ml) using qPCR and RT-PCR, and levels of TGF-β and NO in the LPS- and ANGPTL4-treated primary macrophages (n = 5). Scale bar represents 100 µm"
NCBI gene IDs:
"IL-1β: 16176
IL-6: 16193
TNF-α: 21926"
Figure legend: "B ANGPTL4 and C5a expression levels were measured in bone marrow-derived macrophages from pancreatitis-induced ANGPTL4-/- and WT mice."
NCBI gene IDs:
"ANGPTL4: 57875"
Figure legend: "E C5a, C5aR, TNF-α, IL-6, and IL-1β mRNA levels were identified by qPCR in mouse macrophages or primary acinar cells after treatment with ANGPTL4 and LPS (100 ng/ml). In the transwell experiment, mouse primary acinar cells were cultured in the lower well and macrophages were incubated in the upper transwell bucket treated with ANGPTL4 (100 ng/ml). Expression of ANGPTL4, C5aR, and C5a was determined in siANGPTL4-treated THP1-derived macrophage after LPS activation."
NCBI gene IDs:
"ANGPTL4: 51129
C5aR: 12273
C5a: 15139
IL-1β: 16176
IL-6: 16193
TNF-α: 21926"
Figure legend: "B Immunofluorescence staining of C5a in pancreas tissues from ANGPTL4 -/- and WT animals and macrophage depletion/WT models with pancreatitis. Scale bar represents 150 µm. Levels of C5a were measured in serum (n = 7)."
NCBI gene IDs:
"ANGPTL4: 57875"
Figure legend: "d, ratio between the CPD (copy per  droplets, normalized to γ−actin and Hprt reference genes relative expression) of structural [N,  nucleocapsid] and non-structural [IP4:  RdRp,  RNA-dependent RNA polymerase] viral gene expression  determined by digital droplet PCR  (ddPCR) in the nasal turbinates and in the  lungs at 4 dpi. Data information: Horizontal lines indicate medians. The p value is indicated in bold when significant at a 0.05 threshold. Mann-Whitney test M: male hamsters; F: female hamsters. Data were obtained from two independent experiments for each sex."
NCBI gene IDs:
"Hprt: 
IP4: 
γ−actin: 101839375
nucleocapsid: 43740575
RdRp: 43740578
RNA-dependent RNA polymerase: 43740578"
Figure legend: "E Rate of protein synthesis as measured by the amount of HPG incorporation after 20 mins of differing time intervals (0 - 60 mins) of 5 µM Torin1 for wild type (green) and tor1∆ (yellow). Values are normalised to T = 0 minutes. Exponential one-phase decay non-linear regressions were fitted using the curve fitting function on Prism9. Data aggregated from 3 independent experiments with error bars representing the standard deviations (SD)."
NCBI gene IDs:
"tor1: 2540473"
Figure legend: "B Relative phosphorylation level of the T632 phosphosite identified on Sck1 in the 40-minute time course study. Phosphorylation levels of both wild type (green) and tor1∆ (yellow) are presented relative to the value for wild type cells at T = 0 minutes. Grey dashed line indicates 2-fold threshold."
NCBI gene IDs:
"tor1: 2540473"
Figure legend: "A, B Phosphosites identified on (A) Tif212 and (B) Erf1 respectively showing 2-fold phosphorylation change within 20 minutes of Torin1 (5 µM) addition. Both wild type (green) and tor1∆ (yellow) phosphorylation values are relative to the starting phosphorylation levels before Torin1 addition in wild type cells (T=0 minutes). Grey dashed lines indicate 2-fold threshold."
NCBI gene IDs:
"tor1: 2540473"
Figure legend: "E, F Changes in rates of protein synthesis of the tif471_18A mutant compared to the wild type control (tif471+) upon Torin1 (5 µM) treatment. Residual rates of protein synthesis after 60 minutes of Torin1 treatment was 17% for the tif471_18A phosphomutant and 4.6% for the tif471+ control relative to starting levels in the respective strains. The two graphs represent the untransformed (E) and log2 transformed (F) rates respectively. Error bars represent the standard deviation (SD) of data aggregated from 3 independent experiments."
NCBI gene IDs:
"tif471: 2542290"
Figure legend: "D Distributions of pairwise Ka/Ks (nonsynonymous to synonymous nucleotide substitution rates) ratios between PIWI duplicates in arthropods (n = 17 in Drosophila and n = 37 in others, Mann-Whitney U test, *P&lt;0.05). The center line of the boxplots represents median value, the bounds of the box represent 75th and 25th percentile, and the whiskers represent maximum and minimum value."
NCBI gene IDs:
"PIWI: 34521"
Figure legend: "A PIWI  genes are present in the locust brain. Rp49 served as a loading control (n=4 biological replicates). Data information: data are presented as mean ± SEM."
NCBI gene IDs:
"PIWI: 
Rp49: "
Figure legend: "G Food  intake  by dsPiwi1-treated locusts  and dsGFP-treated control  locusts  within 1 h (n=5 biological replicates) , ***P&lt;0.001 (Student's t-test). Data information: All assays were performed on fourth-instar locusts."
NCBI gene IDs:
"GFP: 
Piwi1: "
Figure legend: "C Representative images of Nile red staining  of lipid droplets in the fat bodies  of dsPiwi1-treated locusts and dsGFP-treated control locusts (n=5 biological replicates). The scale bars represent 50 µm. D Quantification of  lipid droplet staining   in dsPiwi1-treated locusts  and dsGFP-treated control  locusts (n=5 biological replicates). E Data information: All data are presented as the mean ± SEM (Student's t-test, **P&lt;0.01, ***P&lt;0.001, ns: not significant). All assays were performed on four-day-old fourth-instar locusts."
NCBI gene IDs:
"GFP: 
Piwi1: "
Figure legend: "B  Food intake within 1 h following treatment with candidate genes (dsNPF1-treated, n=4 biological replicates; dsCG10621-treated, n=5 biological replicates; dsMFS3-treated, n=5 biological replicates), the data are shown as the mean ± SEM (Student's t-test, **P&lt;0.01)."
NCBI gene IDs:
"CG10621: 
MFS3: 
NPF1: "
Figure legend: "D, E Representative images (D) and quantification (E) of Nile red staining  of fat body lipid droplets in dsNPF1-treated locusts  and dsGFP-treated controls (n=5 biological replicates). The scale bars represent 50 µm, the data are shown as the mean ± SEM (Student's t-test, **P&lt;0.01)."
NCBI gene IDs:
"GFP: 
NPF1: "
Figure legend: "B mRNA levels of differentially  expressed piRNAs located within the flanking region of the NPF1  gene in dsPiwi1-treated locusts and dsGFP-treated control  locusts (n=5 biological replicates). Data information: The  qPCR data are shown as the mean ± SEM (Student's t-test, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001)."
NCBI gene IDs:
"NPF1: 
GFP: 
Piwi1: "
Figure legend: "C The mRNA expression  of NPF1 was measured by qPCR after a mixture of inhibitors or mimics of piRs-3-I2 and piRs-3-I3 was injected (n=5 biological replicates). Data information: The qPCR  data are shown as the mean ± SEM (Student's t-test, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001)."
NCBI gene IDs:
"NPF1: 
piRs-3-I2: 
piRs-3-I3: "
Figure legend: "E  Body weight  measurement of locusts administered inhibitors (NC, n=25  locusts; Inhibitors- piRs-3-I2+I3, n=23 locusts) and mimics (NC, n=37 locusts; Inhibitors-piRs-3-I2+I3, n=39 locusts) of  piRs-3-I2 and piRs-3-I3. The center line of the boxplots represents median value, the bounds of the box represent 75th and 25th percentile, and the whiskers represent maximum and minimum value."
NCBI gene IDs:
"piRs-3-I2 : 
piRs-3-I2: 
piRs-3-I3: "
Figure legend: "F  RNA-binding protein  immunoprecipitation assay of piRs-3-I2 and piRs-3-I3 in Piwi1 immunoprecipitates from brain tissue extracts (n=6 biological replicates). Normal mouse IgG was used as a negative control."
NCBI gene IDs:
"piRs-3-I2: 
piRs-3-I3: "
Figure legend: "A U6 RNA was used as a nuclear RNA control (relative value set to 1 in the nuclear  fraction), and glycine tRNA was used as the cytoplasmic  RNA control (relative value set to 1 in the  cytoplasmic fraction) (n=4 biological replicates), U6 and   tRNA-Gly  was used as an endogenous control."
NCBI gene IDs:
"U6: "
Figure legend: "B Relative expressions of piRs-3-I2 and piRs-3-I3 determined by qPCR and high-throughput sequencing in the  nucleus (n=3 biological replicates). The brain tissues were homogenized and then divided into two parts of equal volume; one was prepared for high-throughput sequencing  and the other was used for piRNA quantification in nuclear and cytoplasmic fractions after subcellular RNA fractionation. The relative piRNA expression in  nucleus was determined by multiplying the relative nuclear piRNA fraction estimate by  qPCR quantification by the normalized count of piRNA in high-throughput sequencing libraries. Data information: The  qPCR data are shown as the mean ± SEM (Student's t-test, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001)."
NCBI gene IDs:
"piRs-3-I2: 
piRs-3-I3: "
Figure legend: "C  Expression level of pre-NPF1 in the nucleus after a mixture of inhibitors (piRs-3-I2  and piRs-3-I3) was injected (n=4 biological replicates). D qPCR expression analyses of  pre-NPF1. The precursor mRNA expression  level was measured by  qPCR  after the dsPiwi1 treatments (n=6 biological replicates). E Data information: The qPCR data are shown as the mean ± SEM (Student's t-test, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001)."
NCBI gene IDs:
"pre-NPF1: 
piRs-3-I2: 
piRs-3-I3: 
Piwi1: "
Figure legend: "F Analysis was performed to amplify  pre-NPF1 and piRs-3-I3 in hnRNP F/H  immunoprecipitates from extracts of brain tissue treated with  piRs-3-I3 mimics compared to the negative controls (pre-NPF1  n=3 biological replicates; piRs-3-I3  n=4 biological replicates). G Analysis was performed to amplify pre-NPF1 in U2AF65 immunoprecipitates from extracts of brain tissue treated with  piRs-3-I3  mimics compared to the negative controls (pre-NPF1 n=3 biological replicates). H"
NCBI gene IDs:
"pre-NPF1: 
piRs-3-I3: "
Figure legend: "J Expression level of the mature  NPF1 gene and pre-NPF1 in 293T  cells transfected with pre-NPF1 and mutated pre-NPF1 (n=5 biological replicates). NC means negative control. The center line of the boxplots represents median value, the bounds of the box represent 75th and 25th percentile, and the whiskers represent maximum and minimum value. Data information: The qPCR  data are shown as the mean ± SEM (Student's t-test, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001)."
NCBI gene IDs:
"NPF1: 
pre-NPF1: "
Figure legend: "ChEP analysis using EGFP (control)- or Piwi-KD OSCs. Signals with an adjusted p-value (Bonferroni test) of less than 0.05 are highlighted in red. The signals for Lam, LamC, and H1 are labelled within the plots."
NCBI gene IDs:
"EGFP: 
Piwi: 34521"
Figure legend: "Lam- or LamC-KD followed by qRT-PCR of RNA levels for piRNA target (mdg1 and gypsy) or non-target (roo) TEs. Error bars indicate SD (n=3). * = p-value&lt;0.05 (t-test). Note that the complete depletion of Lam and LamC is impossible since their KD causes severe damage to cells."
NCBI gene IDs:
"mdg1: 
roo: 
Lam: 33782
LamC: 36615
gypsy: 41740"
Figure legend: "Boxplots showing fold changes in the expression of piRNA target or non-target TEs based on RNA-seq upon Piwi-KD (left), and the frequency of piRNAs, which target piRNA target TEs or non-piRNA target TEs (right). Definition of piRNA target and non-target TEs is in Fig EV1E. Boxplot whiskers, box, and central band show maximum, third quartile, median, first quartile, and minimum, respectively (n=10 for piRNA target TEs, n= 80 for non-piRNA target TEs). P-values were calculated using the Wilcoxon rank-sum test."
NCBI gene IDs:
"Piwi: 34521"
Figure legend: "Boxplot showing DamID-seq signals of piRNA target or non-target TEs upon EGFP (control)- or Piwi-KD. Boxplot whiskers, box, and central band show maximum, third quartile, median, first quartile, and minimum, respectively (n=10 for piRNA target TEs, n= 80 for non-piRNA target TEs). P-values were calculated using the Wilcoxon rank-sum test."
NCBI gene IDs:
"EGFP: 
Piwi: 34521"
Figure legend: "Oligo-FISH images for the representative piRNA target region described in Fig 4A, upon EGFP (control)- or Piwi-KD. Gray indicates DAPI staining for DNA, magenta indicates FISH signal, and green indicates Lamin immunofluorescence staining. Scale bar, 5μm. Ratio of distance from FISH signal to the nuclear periphery (DAPI surface) is quantified at right, dot in the box plot indicates mean value; Boxplot whiskers, box, and central band show minimum, first quartile, median, third quartile, and maximum, respectively. P-values calculated with Mann-Whitney U test."
NCBI gene IDs:
"EGFP: 
Piwi: 34521"
Figure legend: "Oligo-FISH images for changes in the intra-TAD interaction at the TAD described in (C), upon EGFP (control)- or Piwi-KD. Gray indicates DAPI staining for DNA, green and magenta indicates FISH signal at the different positions of TADs (TAD-1 and TAD-2). Details of TAD probe design is described in Fig EV2C. Scale bar, 5μm. Distance between two FISH signals is quantified at right, dot in the box plot indicates mean value; Boxplot whiskers, box, and central band show minimum, first quartile, median, third quartile, and maximum, respectively. P-values calculated with Mann-Whitney U test."
NCBI gene IDs:
"EGFP: 
Piwi: 34521"
Figure legend: "Boxplots showing fold changes in the histone mark association of piRNA target or non-target TEs based on ChIP-seq upon Piwi-KD (Definition of piRNA target and non-target TEs are described in Fig EV1E). Boxplot whiskers, box, and central band show maximum, third quartile, median, first quartile, and minimum, respectively (n=10 for piRNA target TEs, n= 80 for non-piRNA target TEs). P-values were calculated by Wilcoxon rank-sum test."
NCBI gene IDs:
"Piwi: 34521"
Figure legend: "ChIP‐qPCR analysis of H3K27Ac and H3K4me3 occupancy on the reporter gene upon transfection of λN-Nxf2 expression vector or control EGFP expression vector. Bar graph shows the occupancy relative to that of the sample transfected with a control plasmid. Error bars indicate SD (n = 3), * = p-value&lt;0.01 (t-test).."
NCBI gene IDs:
"EGFP: 
Nxf2: 39843"
Figure legend: "Density plots for DamID-seq signals over consensus sequences from piRNA target (gypsy and mdg1, orange) and non-target TEs (roo, blue) in EGFP (control)- or Nxf2-KD cells."
NCBI gene IDs:
"EGFP: 
mdg1: 
roo: 
Nxf2: 39843
gypsy: 41740"
Figure legend: "Oligo-FISH images for the reporter of the tethering system, at 0h (control), 48h hours, 96 hours after transfection of λN-Nxf2 expression vector. Gray indicates DAPI staining for DNA, magenta indicates FISH signal, and green indicates Lamin staining. Scale bar, 5μm. The ratio of distance from FISH signal to the nuclear periphery (DAPI surface) is quantified at right, dot in the box plot indicates mean value; Boxplot whiskers, box, and central band show minimum, first quartile, median, third quartile, and maximum, respectively. P-values calculated with Mann-Whitney U test."
NCBI gene IDs:
"Nxf2: 39843"
Figure legend: "Dot plot showing the correlation between the normalized density of indicated TEs per TAD (x-axis) and changes in intra-TAD interactions upon H1(left)-, Egg(middle)-, or HP1a(right)-KD (y-axis). Trend lines are in red. Spearman's rank correlation rho is indicated along with the p-value (algorithm AS 89). n.s.; not significant (p &gt; 0.05) Dot plot showing the correlation between the normalized density of indicated TEs per TAD (x-axis) and changes in inter-TAD interactions upon H1(left)-, Egg(middle)-, or HP1a(right)-KD (y-axis). Trend lines are in red. Spearman's rank correlation rho is indicated along with the p-value (algorithm AS 89). n.s.; not significant (p &gt; 0.05)"
NCBI gene IDs:
"Egg: 37962
H1: 3772225
HP1a: 34119"
Figure legend: "(B) real time qPCR from brain lysates showing higher PTX3 expression in late embryonic and early postnatal brain, and lower in the adult brain. Data are presented as mean±SEM (B) qPCR fold change normalized on E15, E15=1.037±0.154, P0=0.711±0.094, P7=0.472±0.0524, P14=0.135±0.007, P28=0.168±0.020, P90=0.183±0.029. One-way ANOVA analysis of variance followed by post hoc Tukey test: E15 vs P7 ***p=0.0001, E15 vs P14 **** p&lt;0.0001, E15 vs P2 ****p&lt;0.0001, E15 vs P90 ****p&lt;0.0001; P0 vs P14 $$$p=0.0007, P0 vs P28 $$p=0.001, P0 vs P90 $$p=0.002; 4 animals for each time point"
NCBI gene IDs:
"PTX3: 689388"
Figure legend: "Quantitation of PTX3 levels in pure astrocyte and neuronal cultures (D) rea time qPCR performed on astrocyte and neuronal lysates qPCR astro=1.167±0.288; neu=0.148±0.057; Mann-Whitney test **p=0.0095. 2 independent neuronal cultures evaluated. Data are presented as mean±SEM"
NCBI gene IDs:
"PTX3: 689388"
Figure legend: "H) Representative images showing 14DIV WT and TSG6 KO neurons stained for surface AMPARs (GluA, green), the presynaptic protein Bassoon (blue) and tubulin (red) in the different tested conditions. Arrowheads point to postsynaptic GluA clusters. Scale bar: 5µm"
NCBI gene IDs:
"TSG6: 84397"
Figure legend: "I) Synaptic surface GluA quantitation showing no effect of PTX3 treatment in TSG6 KO cultures. On the contrary WT cultures (from littermates) display increased surface GluA&amp;Bsn/Bsn upon PTX3 treatment. A significant enhancement of surface GluA receptors was induced by TTX in both TSG6 KO and WT cultures (WT=1±0.06; WT+PTX3=1.389±0.113; WT+TTX=1.698±0.109; Number of fields examined: 40, 39, 32 respectively; Kruskall-Wallis test p&lt;0.0001 followed by post hoc Dunn&quot;s test. TSG6 KO=1.000±0.047; TSG6 KO+PTX3=0.979±0.048; TSG6 KO+TTX=1.363±0.077. Number of fields examined: 54, 57, 29 respectively; one-way ANOVA analysis of variance, p&lt;0.0001 followed by post hoc Tukey test as indicated in figure. n=3 independent experiments, data are presented as normalized mean values"
NCBI gene IDs:
"TSG6: 84397"
Figure legend: "J) Representative mEPSC traces recorded from WT and TSG6 KO littermates cultures treated or not with PTX3 (1μg/ml for 48hrs). K) On the contrary of WT cultures, mEPSC frequency quantitation shows no increase in frequency in TSG6 KO cultures when treated with PTX3(Normalized Frequency, WT Ctr: 1.000±0.074; Wt+PTX3=1.912±0.289; TSG6 KO Ctr=1±0.079; TSG6 KO+PTX3=1.226±0.096; 22, 20, 30, 24 cells respectively, 6 TSG6 KO mice and 4 WT littermates. One way ANOVA test p=0.002 followed by post hoc Dunn's test, data are presented as normalized mean±SEM plus the distribution). L) Cumulative probability plot of mEPSC amplitude and average mEPSC amplitude quantitation (inset) showing no difference in amplitude (WT Ctr: 1.000±0.034, Wt+PTX3=1.069±0.046, TSG6 KO Ctr =1.000±0.028; TSG6 KO+PTX3=1.074±0.67; 22, 20, 30, 24 cells respectively, 6 TSG6 KO mice and 4 WT littermates. One way ANOVA test p=0.817 followed by post hoc Dunn's test, data are presented as normalized mean±SEM plus the distribution. Cumulative distribution is analyzed by Kolmogorov-Smirnov test). "
NCBI gene IDs:
"TSG6: 84397"