EMBO/SourceData huggingface dataset:
Figure legend: "C. Cumulative distribution showing fold changes (FCs) in transcript abundance in control morphants (blue curves) and maternal zRbm14 morphants (red curves) between the indicated stages. Transcript abundances were averaged from two independent experiments."
NCBI gene IDs:
"Rbm14: 323721///402858"
Figure legend: "E. Cumulative distribution showing FCs of transcript abundance of the indicated clusters between control and maternal zRbm14 morphants."
NCBI gene IDs:
"Rbm14: 402858///323721"
Figure legend: "F. Relative transcript levels of three typical maternal genes. FCs were calculated relatively to the levels in 2.5-hpf ctrl-MO embryos. org, oogenesis-related; trip10a, thyroid hormone receptor interactor 10a; dnajc5ga, DnaJ (Hsp40) homolog, subfamily C, member 5 gamma a."
NCBI gene IDs:
"DnaJ (Hsp40) homolog: 322863
dnajc5ga: 322863
member 5 gamma a: 322863
subfamily C: 322863
oogenesis-related: 100001110
org: 100001110
thyroid hormone receptor interactor 10a: 492762
trip10a: 492762"
Figure legend: "G. Whole-mount in situ RNA hybridization of org, trip10a, and dnajc5ga. A representative embryo is shown for each group of at least 26 embryos."
NCBI gene IDs:
"dnajc5ga: 322863
org: 100001110
trip10a: 492762"
Figure legend: "Depletion of zRbm14 resulted in accumulation of poly(A)-containing maternal mRNAs. Total RNAs purified from the indicated zebrafish embryos were subjected to poly(A) length assays. One set of representative PCR results for org and trip10a (B) As the PCR products of the poly(A)-transcripts mainly emerged as a single band, only intensities of this major band were measured."
NCBI gene IDs:
"org: 100001110
Rbm14: 402858///323721
trip10a: 492762"
Figure legend: "Depletion of zRbm14 resulted in accumulation of poly(A)-containing maternal mRNAs. Total RNAs purified from the indicated zebrafish embryos were subjected to poly(A) length assays. quantification results from three independent experiments (C) are presented. As the PCR products of the poly(A)-transcripts mainly emerged as a single band, only intensities of this major band were measured."
NCBI gene IDs:
"Rbm14: 402858///323721"
Figure legend: "D. zParn associated with zRbm14b. GFP-zRbm14b was co-expressed respectively with the indicated RFP fusion proteins in HEK293T cells. Co-immunoprecipitations (co-IP) were performed using anti-GFP beads. Luciferase (Luc)-RFP served as a negative control."
NCBI gene IDs:
"Luc: 
Luciferase: 
RFP: "
Figure legend: "J. zRbm14b and zParn efficiently co-phase separated in deadenylation experiments. The addition of SDS disrupted the condensates to terminate the deadenylation reaction."
NCBI gene IDs:
"Parn: 791461
Rbm14b: 402858"
Figure legend: "Co-phase separation with zRbm14b markedly enhanced the deadenylation activity of zParn. RNAs extracted from the indicated samples were subjected to immuno-northern blotting using an antibody against m6A (K). were quantified from five sets of independent results."
NCBI gene IDs:
"Parn: 791461
Rbm14b: 402858"
Figure legend: "Co-phase separation with zRbm14b markedly enhanced the deadenylation activity of zParn. Relatively proportions of Cap-10m6A-A0 in total RNAs (L) were quantified from five sets of independent results."
NCBI gene IDs:
"Parn: 791461
Rbm14b: 402858"
Figure legend: "D. Relative transcript levels of Rbm14 in the indicated E4.5 blastocysts based on RNA deep Sequencing results, normalized to those of Gapdh. Six blastocysts were considered as Rbm14-/- embryos because they contained only trace amount of Rbm14 transcript reminiscent of maternal mRNA."
NCBI gene IDs:
"Gapdh: 14433
Rbm14: 56275"
Figure legend: "Severe down-regulation of Gata6 and Nanog in Rbm14-depleted mouse blastocysts. Representative immunofluorescent images (H) were from single option sections. DAPI stained nuclear DNA. As Gata6, Nanog, and Rbm14 localize to the nucleus in blastocysts"
NCBI gene IDs:
"Rbm14: 56275"
Figure legend: "Severe down-regulation of Gata6 and Nanog in Rbm14-depleted mouse blastocysts. As Gata6, Nanog, and Rbm14 localize to the nucleus in blastocysts, their relative intensity in a cell (I) was quantified as their nuclear immunofluorescent intensity normalized with fluorescent intensity of the nuclear DNA. Five brightest cells were quantified for each embryo."
NCBI gene IDs:
"Rbm14: 56275"
Figure legend: "CSF PGRN levels do not differ among MC participants carrying a PSEN1, PSEN2 or APP mutation."
NCBI gene IDs:
"APP: 351
PSEN1: 5663
PSEN2: 5664"
Figure legend: "(A) Serum levels of miR-483-3p/-5p in IPAH patients (n = 139) and HC (n = 95) measured by qPCR. The data are fold change normalized to the averaged level of HC. Data information: Values are expressed as median ± interquartile range. Statistical test: Mann Whitney U test (A)"
NCBI gene IDs:
"-5p: 619552
miR-483-3p: 619552"
Figure legend: "(B) ROC curve with sensitivity and specificity of serum levels of miR-483-3p/-5p for differentiating IPAH patients from HCs at diagnosis (IPAH, n = 139; HC, n = 95). Data information: Values are expressed as median ± interquartile range."
NCBI gene IDs:
"-5p: 619552
miR-483-3p: 619552"
Figure legend: "(C) Levels of miR-483-3p/-5p associated with PAH risk in 3 groups. IPAH patients were divided into a low risk group (Low) and an intermediate plus high risk group (Inter&amp;high) according to the World Symposium on Pulmonary Hypertension 2018 [14]. Data information: Values are expressed as median ± interquartile range. Statistical test: Kruskal-Wallis U test (C)."
NCBI gene IDs:
"-5p: 619552
miR-483-3p: 619552"
Figure legend: "(D) Levels of miR-483-3p were inversely correlated with serum levels of ET-1 (IPAH, n = 118; HC, n = 95) and IL-6 (IPAH, n = 112; HC, n = 93). Data information: Values are expressed as median ± interquartile range."
NCBI gene IDs:
"miR-483-3p: 619552"
Figure legend: "(A) CD144-enriched EVs were isolated from serum of IPAH patients (n = 37) and HCs (n = 34). The levels of miR-483-3p/-5p were measured by qPCR. Data information: Values are expressed as median ± interquartile range. Statistical test: Mann Whitney U test."
NCBI gene IDs:
"-5p: 619552
miR-483-3p: 619552"
Figure legend: "PAECs were transfected with miR-483-3p/-5p mimic or scramble RNA for 36 hr before RNA isolation and then analyzed by RNA-seq. Data are results from two biological repeats. (C) Heat map comparison of log2 fold changes of the indicated genes. Data information: Values are expressed as median ± interquartile range. Statistical test: Mann Whitney U test."
NCBI gene IDs:
"-5p: 619552
miR-483-3p: 619552"
Figure legend: "PAECs were infected with Lenti-pre-miR-483 or Lenti-null for 24 hr. Expression levels of miR-483-3p/-5p measured by qPCR Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"-5p: 
miR-483-3p: 
pre-miR-483: 619552"
Figure legend: "PAECs were infected with Lenti-pre-miR-483 or Lenti-null for 24 hr. Expression levels of TGF-β, TGFBR2, β-catenin, CTGF, IL-1β, and ET-1 mRNA were measured by qPCR Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"CTGF: 1490
β-catenin: 1499
ET-1: 1906
IL-1β: 3553
pre-miR-483: 619552
TGF-β: 7040
TGFBR2: 7048"
Figure legend: "PAECs were infected with Lenti-pre-miR-483 or Lenti-null for 24 hr. Expression levels of TGF-β, TGFBR2, β-catenin, CTGF, IL-1β, and ET-1 protein were measured by Western blot Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"pre-miR-483: 619552"
Figure legend: "(E) Bovine aortic ECs were transfected with a luciferase reporter fused with the 3'UTR of TGF-β (Luc-TGF-β WT), TGFBR2 (Luc-TGFBR2 WT), IL-1β (Luc-IL-1β WT) or ET-1 (Luc-ET-1 WT) or a binding site mutation (Luc-TGF-β Mut, Luc-TGFBR2 Mut, Luc-IL-1β Mut, ET-1-Mut), then infected with Lenti-pre-miR-483 for additional 36 hr. Luciferase activity was measured. Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"Luc: 
luciferase: 
ET-1: 1906
IL-1β: 3553
pre-miR-483: 619552
TGF-β: 7040
TGFBR2: 7048"
Figure legend: "PAECs were infected with Lenti-pre-miR-483 or Lenti-null for 36 hr. The Ago1- or Ago2-associated miRNAs and mRNAs were enriched by immunoprecipitation with anti-Ago1 or anti-Ago2. Levels of miR-483-3p/-5p mRNA were detected by qPCR and normalized to those of Ago1 or Ago2 protein. Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"-5p: 
miR-483-3p: 
pre-miR-483: 619552"
Figure legend: "PAECs were infected with Lenti-pre-miR-483 or Lenti-null for 36 hr. The Ago1- or Ago2-associated miRNAs and mRNAs were enriched by immunoprecipitation with anti-Ago1 or anti-Ago2. Levels of TGF-β, TGFBR2, β-catenin, CTGF, IL-1β, and ET-1 mRNA were detected by qPCR and normalized to those of Ago1 or Ago2 protein. Data information: Values are expressed as mean ± SEM from 3 independent experiments. Statistical test: t-test (*P &lt; 0.05 between the indicated groups"
NCBI gene IDs:
"CTGF: 1490
β-catenin: 1499
ET-1: 1906
IL-1β: 3553
pre-miR-483: 619552
TGF-β: 7040
TGFBR2: 7048"
Figure legend: "(A) Lung ECs were isolated from EC- miR-483 transgenic rats (Tg) and their wild-type littermates (WT), n = 3 in each group. MiR-483-3p/-5p levels were measured by qPCR. Data information: Values are expressed as mean ± SEM."
NCBI gene IDs:
"-5p: 100314198
miR-483: 100314198
MiR-483-3p: 100314198"
Figure legend: "(B) Serum from WT and Tg rats (n = 6) were collected. MiR-483-3p and -5p associated with CD144 were enriched by immunoprecipitation with anti-CD144. Levels of miR-483-3p/-5p were measured by qPCR. Data information: Values are expressed as mean ± SEM. "
NCBI gene IDs:
"-5p: 100314198
MiR-483-3p: 100314198
miR-483-3p: 100314198"
Figure legend: "Lung ECs isolated from miR-483-Tg rat and WT littermates were exposed to normoxia or hypoxia (0.2% O2) for 24 hr. Proliferation of ECs were measured by flow cytometry Data information: Values are expressed as mean ± SEM. Data are representative of three independent experiments Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups"
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "Lung ECs isolated from miR-483-Tg rat and WT littermates were exposed to normoxia or hypoxia (0.2% O2) for 24 hr. migration of ECs were measured by wound healing assay Data information: Values are expressed as mean ± SEM. Data are representative of three independent experiments Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups"
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "Tg and WT rats were injected with saline or MCT. Expression levels of TGF-β, TGFBR2, β-catenin, CTGF, IL-1β, and ET-1 mRNA and protein in lung tissues were measured by qPCR and Western blot, respectively (E). Data information: Values are expressed as mean ± SEM."
NCBI gene IDs:
"CTGF: 29576
β-catenin: 84353
ET-1: 24323
IL-1β: 24494
TGF-β: 59086
TGFBR2: 81810"
Figure legend: "Tg and WT rats were injected with saline or MCT. Levels of miR-483-3p/-5p in serum and lung tissues were measured by qPCR (F). Data information: Values are expressed as mean ± SEM."
NCBI gene IDs:
"-5p: 100314198
miR-483-3p: 100314198"
Figure legend: "EC-miR-483-Tg and WT rats were injected with saline or MCT. Analysis of mPAP for the indicated groups (7 rats per group) Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups)."
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "EC-miR-483-Tg and WT rats were injected with saline or MCT. Analysis of RV hypertrophy [RV/(LV+S)] for the indicated groups (7 rats per group) Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups)."
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "EC-miR-483-Tg rats and WT littermates were injected with SU5416 and then exposed to hypoxia for 3 weeks and had reoxygenation for 2 weeks or injected with DMSO and exposed to normoxia for 5 weeks. Analysis of mPAP (E) for the indicated groups. Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups)."
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "EC-miR-483-Tg rats and WT littermates were injected with SU5416 and then exposed to hypoxia for 3 weeks and had reoxygenation for 2 weeks or injected with DMSO and exposed to normoxia for 5 weeks. Analysis of RV hypertrophy [RV/(LV+S)] (F) for the indicated groups. Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups)."
NCBI gene IDs:
"miR-483: 100314198"
Figure legend: "qPCR analysis of miR-483-3p/-5p in serum from PH (MCT-treated) rats. Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups"
NCBI gene IDs:
"-5p: 
miR-483-3p: "
Figure legend: "qPCR analysis of miR-483-3p/-5p in lung tissue from PH (MCT-treated) rats. Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups"
NCBI gene IDs:
"-5p: 
miR-483-3p: "
Figure legend: "(D) Protein and mRNA levels of TGF-β, TGFBR2, β-catenin, CTGF, IL-1β, and ET-1 measured by Western blot and qPCR, respectively (n = 3×3, samples were pooled from three animals for each assay, and 3 independent experiments [a total of 9 animals] were performed). Data information: Values are expressed as mean ± SEM. Statistical test: t-test (*P &lt; 0.05 vs. respective controls or between the indicated groups"
NCBI gene IDs:
"CTGF: 29576
β-catenin: 84353
ET-1: 24323
IL-1β: 24494
TGF-β: 59086
TGFBR2: 81810"
Figure legend: "(A) U2OS  (left panel), RPE1-hTERT (middle panel), and  HeLa  cells (right panel) were monitored for their sensitivity to the radiomimetic drug NCS using the SRB assay. For each cell line, the following conditions were used: U2OS cells were transfected with a nontargeting siRNA (siCtrl) or an siRNA targeting human POGZ  (siPOGZ-1 or -2); RPE1-hTERT cells were transduced a control shRNA (shCtrl) or a shRNA directed against  human POGZ (shPOGZ-1 or -2); HeLa cells expressing a non-targeting sgRNA (sgCtrl) or a sgRNA targeting human POGZ and sub-cloned (POGZ∆-1 or -2). Cells were pulsed with NCS at the indicated concentrations for 1 hour, replenished with fresh medium and incubated for 4 days before being processed for SRB assays. Data are represented as a bar graph showing the relative mean ± SEM, each replicate being representing as a round symbol (3 biological replicates). Significance was determined by two-way ANOVA followed by a Bonferroni's test. *P&lt;0.01."
NCBI gene IDs:
"POGZ: 23126"
Figure legend: "(D) U2OS (left panel) and HeLa (right panel) cells containing the DR-GFP reporter construct were transfected with the indicated siRNA. 24h post-transfection. Cells were transfected with the I-SceI  expression plasmid or an empty vector (EV), and the GFP+ population was analyzed 48h post-plasmid transfection. The percentage of  GFP+ cells was determined for each individual condition and subsequently normalized to the non-targeting condition provided with  I-SceI  (siCtrl, I-SceI). Data are represented as the mean ± SEM, each replicate being representing as a round symbol (n=3 biological replicates). Significance was determined by one-way ANOVA followed by a Dunnett's test. *P≤0.0001."
NCBI gene IDs:
"I-SceI: 854590"
Figure legend: "(F) Quantification of γ-H2AX  foci in  HeLa  cells where POGZ has been targeted by  CRISPR  technology (POGZ∆-1 or -2) and in control HeLa  cells (sgCtrl). Cells were exposed to 1 Gy before being pulsed with  Edu for 1hr and were recovered at the indicated time points. Cells were fixed, stained, and imaged via confocal microscopy. Data are the total number of γ-H2AX foci in EdU+ cells and represented as a bar graph showing the mean ± SD (n = 3 biological replicates, with at least 100 cells analyzed for each  time point). Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.05, **P&lt;0.0005. (G) Representative images used for quantification in (F). Scale bar = 5 μm. "
NCBI gene IDs:
"CRISPR: 
POGZ: 23126"
Figure legend: "(A) U2OS stably expressing mCherry-LacR-FokI were transfected with the indicated siRNA. 24h post-transfection, DNA damage was induced using Shield1 and 4-OHT. Immunofluorescence  against the indicated DNA repair proteins was subsequently performed to monitor their accumulation at sites of DNA damage by confocal microscopy. Data are represented as a box-and-whisker (10-90 percentile) where the fluorescent signal at the mCherry focus was normalized to nuclear  background. At least 100 cells per condition were counted (n=3 biological replicates). Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.05, **P&lt;0.005, **P≤0.0005."
NCBI gene IDs:
"FokI: 
LacR: 
mCherry: "
Figure legend: "(B)  U2OS  (left panel), RPE1-hTERT (middle panel), and HeLa cells (right panel) were monitored for their capacity to form IR-induced BRCA1 foci. For each cell line, the following conditions were used: U2OS cells were transfected with a nontargeting siRNA (siCtrl) or an siRNA targeting human POGZ (siPOGZ-1 or -2); RPE1-hTERT  cells were transduced a control shRNA (shCtrl) or a shRNA directed against  human POGZ (shPOGZ-1 or -2); HeLa cells were expressing a non-targeting sgRNA (sgCtrl) or a sgRNA targeting human POGZ and sub-cloned (POGZ∆-1 or -2). Cells were exposed to 1 Gy before being pulsed with  Edu for 1hr and were recovered 1h post-exposure to IR. Cells were fixed, stained, and imaged via confocal microscopy. Data are the total number of ΒRCA1 foci in EdU+ cells and represented as a bar graph showing the mean ± SD (n = 3 biological replicates, with at least 100 cells analyzed for each time point). Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.005, **P&lt;0.001."
NCBI gene IDs:
"POGZ: 23126"
Figure legend: "(E) U2OS stably expressing  mCherry-LacR-FokI were transduced with the indicated shRNA and subsequently transfected with the indicated siRNA. 24h post-transfection, DNA damage was induced using Shield-1 and  4-OHT. Immunofluorescence against BRCA1 was subsequently performed to monitor its accumulation at sites of DNA damage by confocal microscopy. Data are represented as a box-and-whisker (10-90 percentile) where the fluorescent signal at the mCherry focus was normalized to nuclear  background. At least 50 cells per condition were counted. Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.0001."
NCBI gene IDs:
"FokI: 
LacR: 
mCherry: "
Figure legend: "(G)  U2OS stably expressing mCherry-LacR-FokI were transduced with the indicated siRNA. 24h post-transfection, DNA damage was induced using Shield-1  and 4-OHT. Immunofluorescence  against the indicated  HP1 isoform was subsequently performed to monitor its respective accumulation at sites of DNA damage by  confocal microscopy. Data are represented as a box-and-whisker (10-90 percentile) where the fluorescent signal at the mCherry focus was normalized to nuclear  background. At least 75 cells per condition were counted. Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.005, **P&lt;0.0001."
NCBI gene IDs:
"FokI: 
LacR: 
mCherry: "
Figure legend: "(C)  HeLa cells transfected with the indicated construct were monitored for their capacity to form  IR-induced BRCA1 foci. HeLa cells where POGZ has been targeted by  CRISPR technology (POGZ∆-1) and in control HeLa cells (sgCtrl) were transfected by an empty Flag vector (EV) or a sgRNA-resistant FLAG-tagged POGZ  cDNA construct corresponding to indicated rescue mutant (full-length, FL; POGZ801-848, HPZ). Cells were exposed to 1 Gy before being pulsed with  Edu for 1hr and were recovered 1h post-exposure to  IR. Cells were fixed, stained, and imaged via confocal microscopy. Data are the total number of ΒRCA1 foci in EdU+ cells and represented as a bar graph showing the mean ± SD (n = 3 biological replicates, with at least 100 cells analyzed for each time point). Significance was determined by two-way ANOVA followed by a Dunnett's test. *P&lt;0.05, **P&lt;0.0001. (D) Representative images used for quantification in (C). Scale bar = 5 μm. "
NCBI gene IDs:
"CRISPR: 
Flag: 
FLAG: 
POGZ: 23126"
Figure legend: "Observed genotypic distribution  of offspring of heterozygous Pogz+/∆ crosses at specified embryonic day. Each litter is considered a biological replicate. Data are represented as a bar graph showing the mean ± SEM (n for each embryonic day is specified). (D)"
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "(E) The body mass of male wild-type (WT) or Pogz+/∆ mice was monitored weekly for 4 weeks post-weaning. Data are represented as a bar graph showing the mean ± SEM and each mouse  is represented by a round dot (WT) or a square (Pogz+/∆ ). At least 4 mice per genotype was monitored. Significance was determined by two-way ANOVA followed by a Sidak's test. *P&lt;0.05."
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "(H) Quantification of the  distance travelled (left panel) and the average speed (right panel) of each mouse (n=6 mice per genotype) in the open field. Data are represented as a bar graph showing the mean ± SEM and each mouse is represented by a a round dot (WT) or a square (Pogz+/∆ ). Significance was determined by unpaired two-tailed t-test. *P&lt;0.005."
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "(L) Plasma was isolated from  cardiac punctures of wild-type (WT) or Pogz+/∆ mice (8 weeks) and assessed for circulating levels of specified immunoglobulin isotypes. Each mouse is represented by a round dot (WT) or a square (Pogz+/∆ ) (n=6 mice per genotype). Significance was determined by two-way ANOVA followed by a Bonferroni's test. *P&lt;0.005."
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "(A) Wild-type (WT) and Pogz+/∆ mice were subjected to a lethal dose of ionizing radiation (8.5 Gy) before recovering in sterile conditions and being assessed for their sensitivity to  IR. Data are represented as a Kaplan-Meier survival curve of each genotype ((n=6 mice per genotype). Significance was determined by log-rank (Mantel-Cox) test. *P&lt;0.005."
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "(D) MEFs  were monitored for their sensitivity to the radiomimetic drug phleomycin using the SRB assay. Immortalized MEFS obtained from the indicated genotype were treated with increasing concentrations of phleomycin for 1hr, replenished with fresh medium and incubated for 4 days before being processed for SRB assays. Data are represented as a bar graph showing the relative mean ± SEM, each replicate being representing as a round dot (WT) or a square (Pogz+/∆ ). Significance was determined by two-way ANOVA followed by a Bonferroni's test. *P&lt;0.005, **P&lt;0.0005."
NCBI gene IDs:
"Pogz: 229584"
Figure legend: "Tfeb expression in the vasculature (mice n=10). (A) Representative images of retinal vessels (p5) (A) (scale bar: 50 µm) of Tfeb-EGFP mice stained with anti-iB4 (A) and anti-GFP"
NCBI gene IDs:
"EGFP: 
Tfeb: 21425"
Figure legend: "Tfeb expression in the vasculature (mice n=10). Representative images of glomerular, arterial (scale bars: 10 µm) and interstitial vessels (scale bar: 50 µm) of kidney (p17) of Tfeb-EGFP mice stained with anti-CD31 (B) and anti-GFP"
NCBI gene IDs:
"EGFP: 
Tfeb: 21425"
Figure legend: "Alterations in the embryonic vasculature in TfebEC-/- mice at E10.5 (mice n=25). Representative images of whole-mount embryos and yolk sacs (i, ii) (scale bars: 0.5 mm). Vessels of the head (iii), ocular (iv) and intrasomitic regions (v) were stained with anti-endomucin Ab (scale bars: 100 µm)."
NCBI gene IDs:
"Tfeb: 21425"