EMBO/SourceData huggingface dataset:
Figure legend: "A Heat map from RNA-Seq data from FACS-isolated muscle stem cells and single myofibers showing relative expression of Pax7, Myf5, MyoD, Myog and Myf6."
NCBI gene IDs:
"Myf5: 17877
Myf6: 17878
MyoD: 17927
Myog: 17928
Pax7: 18509"
Figure legend: "C-E Snapshots of the UCSC genome browser showing enrichment of Myf6 ChIP-Seq reads compared to control around EGF (C), LIF and OSM (D), and VEGFA (E) loci superimposed on reads for Histone H3 lysine 4 mono methyl (H3K4me1), Histone H3 lysine 4 tri methyl (H3K4me3) and total H3 in myoblasts (pink) and myotubes (green)."
NCBI gene IDs:
"EGF: 13645
LIF: 16878
OSM: 18413
VEGFA: 22339"
Figure legend: "F Snapshots of representative IGV tracks displaying RNA-Seq data from WT whole muscle (WM), single myofibers (SF) and satellite cells (SC) showing the expression of EGF, EGFR and VEGFA. Ckm and Myf6 are used as positive controls for whole muscle and single fibers. PDGFRA, a marker of fibro/adipogenic progenitor cells, is used as a control to show the lack of niche cells in the single myofiber samples."
NCBI gene IDs:
"PDGFRA: 
Ckm: 12715
EGF: 13645
EGFR: 13649
Myf6: 17878
VEGFA: 22339"
Figure legend: "G-H RNA-Sequencing Reads Per Million (RPM) for EGF (G) and EGFR (H) in satellite cells and single myofibers. RNA-Sequencing libraries were prepared from 1000 satellite cells freshly-isolated by FACS or from a single myofiber as described in the Material and Methods. (n=3 biological replicates per group) bars=mean +/- SD, two-tailed t-test)."
NCBI gene IDs:
"EGF: 13645
EGFR: 13649"
Figure legend: "K Depletion of Myf6 transcript by siRNAs in differentiated primary myotubes (5DM). (n=3 biological replicates), two-tailed t-test, bars = mean +/- SD L Depletion of Myf6 expression by siRNAs leads to a significant reduction in the gene expression output of Epidermal Growth Factor (EGF). (n=3 biological replicates), two-tailed t-test, bars = mean +/- SD"
NCBI gene IDs:
"EGF: 13645
Epidermal Growth Factor: 13645
Myf6: 17878"
Figure legend: "B Picture of 8 week-old Myf6-KO and WT mice."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "C Relative expression of Myf6 transcript in the hindlimb skeletal muscles of Myf6-KO and WT mice measured by Quantitative Real-Time PCR (RT-qPCR) (n=4 mice). Boxes represent 1st and 3rd quartile, central line represents the median, whiskers represent the range, two-tailed t-test."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "D Color map of genes that are up/down regulated in the whole muscle transcriptome (RNA-Seq) of injured and uninjured hindlimb muscles from Myf6-KO and WT counterparts. Differential expression is Log2 transformed and truncated at +/-3. Color scale goes from blue (-3=lower than mean), to yellow (+3=higher than mean). E Heat map showing the distribution of Myf6 peaks within 100kb of the TSS of the myokine genes ranging from zero (black) to six ChIP-Seq peaks (red) occupancy."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "F Expression of EGF protein in the blood serum from Myf6-KO and heterozygous counterparts as measured by Enzyme-Linked Immunosorbent Assay (ELISA). (n=5 heterozygous biological replicates, n=3 Myf6-KO biological replicates), two-tailed t-test, error bars = +/- SD G Expression of VEGFA protein in the blood serum from Myf6-KO and heterozygous counterparts as measured by Enzyme-Linked Immunosorbent Assay (ELISA). (n=5 heterozygous biological replicates, n=3 Myf6-KO biological replicates), two-tailed t-test, error bars = +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "H Expression of VEGFA protein in the secretome of Myf6-KO and WT myotubes as measured by enzyme-linked immunosorbent assay (ELISA). Primary myotubes were derived from MuSCs isolated by Fluorescence Activated Cell Sorting (FACS, see the Extended Material and Methods) from Myf6-KO and WT mice. Myotubes were obtained by feeding &gt;90% confluent primary myoblasts with differentiation media (DMEM supplemented with 5% horse serum) for seven days. Media from the last 48 hours of differentiation (days 5 to 7) was collected for ELISA secretome analysis. Protein readouts were normalized to the total number of nuclei in each plate. (n=4 WT biological replicates, n=3 Myf6-KO biological replicates), two-tailed t-test, error bars = +/- SD I Expression of EGF protein in the secretome of Myf6-KO and WT myotubes as measured by enzyme-linked immunosorbent assay (ELISA). Primary myotubes were derived from MuSCs isolated by Fluorescence Activated Cell Sorting (FACS, see the Extended Material and Methods) from Myf6-KO and WT mice. Myotubes were obtained by feeding &gt;90% confluent primary myoblasts with differentiation media (DMEM supplemented with 5% horse serum) for seven days. Media from the last 48 hours of differentiation (days 5 to 7) was collected for ELISA secretome analysis. Protein readouts were normalized to the total number of nuclei in each plate. (n=4 WT biological replicates, n=3 Myf6-KO biological replicates), two-tailed t-test, error bars = +/- SD J Expression of EGF protein in the secretome of Myf6-HF expressing myotubes derived from Myf6-KO myoblasts (rescue) compared to Myf6-KO myotubes as measured by enzyme-linked immunosorbent assay (ELISA). Primary myotubes were derived from MuSCs isolated by Fluorescence Activated Cell Sorting (FACS, see the Extended Material and Methods) from Myf6-KO mice. Myotubes were obtained by feeding &gt;90% confluent primary myoblasts with differentiation media (DMEM supplemented with 5% horse serum) for five days. Media from the last 48 hours of differentiation (days 3 to 5) was collected for ELISA secretome analysis. Protein readouts were normalized to the total number of nuclei in each plate. (n=3 Myf6-KO technical replicates, n=4 rescue technical replicates), two-tailed t-test, error bars = +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "K RT-qPCR of Myf6 expression in Myf6-KO (n=2 technical replicates), WT (n=3 biological replicates) and Myf6-HF (rescue) myotubes (n=3 technical replicates) (5DM). &quot;Rescue&quot; myotubes were generated from Myf6-KO myoblasts infected with a retrovirus containing Myf6-HF. two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "L-M RT-qPCR of EGF (L) and VEGFA (M) expression in Myf6-KO (n=5) and rescue (n=3-4) primary myotubes (5DM). two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"EGF: 13645
Myf6: 17878
VEGFA: 22339"
Figure legend: "Immunofluorescence staining of WT (N) and Myf6-KO EDL (O) myofibers stained with PAX7, EGFR, and phospho-EGFR antibodies. Live fibers were maintained in culture media for 44 hours, followed by 4 hours of serum starving in non-supplemented DMEM. For treated samples, 40ng/ml of recombinant EGF was added to the non-supplemented DMEM for 10 minutes before fixation. Activation status of EGFR was assessed using antibodies against phospho-EGFR (pEGFR-Y1068). Scale bar=30μm P Quantification of the percentage of all pEGFR+/PAX7+ cells (defined as PAX7+ cells showing low or high pEGFR signal) on WT (n=24 fibers from n=3 mice) , Myf6-KO (n=22 fibers from n=3 mice) and Myf6-KO EDL myofibers treated with EGF (n=14 fibers from n=3 mice). two-tailed t-test, error bars = +/- SD Q Quantification of the percentage of EGFRhigh/PAX7+ cells (defined as PAX7+ cells showing high pEGFR signal) on WT (n=37 fibers from n=3 mice), Myf6-KO (n=34 fibers from n=3 mice) and Myf6-KO EDL myofibers treated with EGF (n=11 fibers from n=3 mice). two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "A Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and Laminin (LAMA1) antibodies at postnatal day 7. Arrowheads indicate PAX7+ satellite cells. Scale bar=200μm B Quantification of the number of PAX7+ cells per unit area (mm2) in TA muscle cross-sections from Myf6-KO and WT counterparts at postnatal day 7. (n=30 fields of view from n=3 animals), two-tailed t-test, error bars = +/- SD. C Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and Laminin (LAMA1) antibodies at postnatal day 21. Arrowheads indicate PAX7+ satellite cells. Scale bar=200μm D Quantification of the number of PAX7+ cells per unit area (mm2) in TA muscle cross-sections from Myf6-KO and WT counterparts at postnatal day 21. (n=27 fields of view from n=3 animals) , two tailed t-test, error bars = +/- SD). E Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and Laminin (LAMA1) antibodies at 3 months of age. Arrowheads indicate PAX7+ satellite cells. Scale bar=200μm F Quantification of the number of PAX7+ cells per unit area (mm2) in TA muscle cross-sections from Myf6-KO and WT counterparts at 3 months of age. (n=52 fields of view from n=5 animals), two tailed t-test, error bars = +/- SD. G Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and Laminin (LAMA1) antibodies at 10 months of age. Arrowheads indicate PAX7+ satellite cells. Scale bar=200μm H Quantification of the number of PAX7+ cells per unit area (mm2) in TA muscle cross-sections from Myf6-KO and WT counterparts at 10 months of age. (n=60 fields of view from n=3 animals), two tailed t-test, error bars = +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "Immunofluorescent analysis of myofiber-associated MuSCs isolated from the EDL muscles of 3 month-old Myf6-KO and WT counterparts. Fibers were stained with PAX7 antibody and counterstained with DAPI at time T=0 hours post fiber isolation. Scale bar=25μm J Quantification of the number of MuSCs per fiber between 3 month-old Myf6-KO (n=41 fibers from n=3 mice) and WT (n=31 fibers from n=3 mice) counterparts. Two-tailed t-test, error bars = +/- SD K Immunofluorescent analysis of myofiber-associated MuSCs isolated from the EDL muscles of 10 month-old Myf6-KO and WT counterparts. Fibers were stained with PAX7 antibody and counterstained with DAPI at time T=0 hours post fiber isolation. Scale bar=25μm L Quantification of the number of MuSCs per fiber between 10 month-old Myf6-KO (n=38 fibers from n=2 mice) and WT (n=67 fibers from n=3 mice) counterparts. Two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and KI67 antibodies at postnatal day 7. Scale bar=100μm, arrowheads highlight select MuSC B Quantification of the percentage of KI67+ over PAX7+ MuSCs between Myf6-KO and WT mice at postnatal day 7 (n=15 fields of view from n=3 animals) Two-tailed t-test, error bars = +/- SD. C Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and KI67 antibodies at postnatal day 21. Scale bar=100μm, arrowheads highlight select MuSC D Quantification of the percentage of KI67+ over PAX7+ MuSCs between Myf6-KO (n=28 fields of view from n=3 animals) and WT mice (n=31 fields of view from n=3 animals) at postnatal day 21. Two-tailed t-test, error bars = +/- SD E Immunofluorescent analysis of TA muscle cross-sections from Myf6-KO and WT mice with PAX7 and KI67 antibodies at 3 months of age. Scale bar=100μm, arrowheads highlight select MuSC F Quantification of the percentage of KI67+ over PAX7+ MuSCs between Myf6-KO and WT mice at 3 months of age (n=30 fields of view from n=5 mice). Two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "H (Left) Heat Map of up and down-regulated genes in the p38 MAPK pathway (WP400) in Myf6-KO and WT satellite cells. (Right) Gene set enrichment analysis of p38 MAPK pathway genes. In each plot, the blue curve represents the gene set enrichment score profile (Subramanian et al., 2005) across p38 MAPK pathway genes that are ranked by their differential expression. The grey curve represents the null expectation. P-values are based on permutation test (10,000 permutations)."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "Immunofluorescent analysis of WT and Myf6-KO primary myotubes cultured in differentiation media for 7 days and stained with MF20 and DAPI. Scale bar=200μm J Quantification of the fusion index between Myf6-KO (n=54 fields of view from n=3 mice) and WT (n=81 fields of view from n=4 mice) 7DM myotubes. Fusion index is calculated as the percentage of myonuclei out of the total number of nuclei per field of view. Two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "EDL myofibers isolated from WT and Myf6-KO mice fixed at time T=0 and stained with PAX7, MYOD and DAPI. Scale bar=30μm L Quantification of the percentage of MYOD+/PAX7+ satellite cells per fiber in Myf6-KO (n= 88 myofibers) vs WT (n= 86 myofibers) myofibers. Two-tailed t-test, error bars = +/- SD M Quantification of the average percentage of MYOD+/PAX7+ satellite cells per mouse in Myf6-KO and WT myofibers (n=3 mice). Two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "Staining for phospho-p38 MAPK on EDL myofibers cultured for 48 hours from Myf6-KO and WT mice. Scale bar=30μm O Quantification of the percentage of phospho-p38+/PAX7+ satellite cells per fiber in Myf6-KO (n=56 myofibers) vs WT (n=67 myofibers) myofibers. Two-tailed t-test, error bars = +/- SD P Quantification of the average percentage of phospho-p38+/PAX7+ satellite cells per mouse in Myf6-KO vs WT myofibers (n=3 mice). Two-tailed t-test, error bars = +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "B Representative images of bioluminescent signal from hindlimbs of Myf6-KO and WT counterparts at day 1, 7, 14, and 21 post transplantation. Scale bar=1cm"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "C Time course of average bioluminescent signal (total flux in radiance) in Myf6-KO and WT mice at 1, 7, 14 and 21 days post MuSC transplantation showing engraftment dynamics of donor MuSCs (mean across 4-5 animals per group ± SEM at each time point, two-tailed t-test)."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "D Bioluminescent signal (radiance) in Myf6-KO and WT mice at each time point normalized to the bioluminescent signal at day 1 post MuSC transplantation. (n=4-5 animals per group ± SD) two-tailed t-test, bars represent mean +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "E-F Immunostaining of TA cross-sections of injured muscle from 3 month-old WT (E) and Myf6-KO (F) mice stained with laminin (LAMA1) and PAX7 antibodies. Acute muscle injury was performed by injection of Cardiotoxin (CTX) into the TA muscle of Myf6-KO and WT mice (n=5 animals per group). Arrowheads indicate PAX7+ MuSCs. Scale bar=100μm G Quantification of the number of PAX7+ cells per unit area of TA muscle cross sections between Myf6-KO and WT mice (n= 5 animals per group) two-tailed t-test, bars represent mean +/- SD"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "H Immunofluorescent analysis of Myf6-KO and WT EDL myofibers cultured in growth media for 48 hours and stained with PAX7 and MYOD antibodies. Scale bar=20μm IQuantification of the number of PAX7+ MuSCs per fiber in Myf6-KO (n=27 fibers from n=3 mice) and WT (n=29 fibers from n=3 mice) mice after 48 hours in culture. Two-tailed t-test, error bars = +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "EdU staining of cultured primary myoblasts isolated by FACS (see materials and methods) from Myf6-KO and WT mice. Staining was analyzed after 6, 12 and 24 hours of EdU incorporation. Scale bar=100μm M Quantification of the percentage of EdU+ myoblasts after 6 hours (WT n=75 fields of view from n=3 mice, Myf6-KO n=72 fields of view from n=3 mice), 12 hours (WT n=82 fields of view from n=3 mice, Myf6-KOn=82 fields of view from n=3 mice), and 24 hours (WT n=70 fields of view from n=3 mice, Myf6-KO n=65 fields of view from n=3 mice) of EdU incorporation. Scale bar=100μm"
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "Representative images of a co-culture assay between WT myotubes and either WT or Myf6-KO myoblasts. Briefly, WT myoblasts were differentiated for 2 days before adding equal numbers of either WT or Myf6-KO myoblasts for an additional 2 days, followed by immunostaining for MF20 and DAPI. Scale bar=400μm O Quantification of the average number of myoblasts per mm2 that have remained mononuclear after their addition to 2DM WT myotubes, for 2 additional days (n=3 biological replicates). Two-tailed t-test, bars represent mean +/- SD."
NCBI gene IDs:
"Myf6: 17878"
Figure legend: "(A) Detection of SARS-CoV-2 in nasopharyngeal swabs and peripheral blood using the GeneFinderTM COVID-19 Plus RealAmp Kit assay (ELITechGroup S.p.A., Turin, Italy). Three viral target genes, RdRp, N, and E, together with the housekeeping gene GAPDH were simultaneously amplified. Here the most sensitive target gene, N, is shown. Samples with Ct values &gt; 40 were defined as negative. A dashed horizontal line indicates the cut-off. IL-6 values are shown in the same graph. Time of baricitinib treatment is highlighted (Patients A, B, and D for 10 days, Patient C for 12 days) with total time of evaluation on the X-axis. Fever and cough symptoms are indicated. The bars in grey indicate that cough has improved but has not resolved."
NCBI gene IDs:
"GAPDH: 
RdRp: 
E: 43740570
N: 43740575"
Figure legend: "A, B Relative gene expression is significantly increased for SARS-CoV-2 MA30 viral E gene (A; p &lt; 0.0017) and interferon response ISG15  (B; p &lt; 0.0044) in Saline  treated infected lung  extracts at 4 days follow up, when compared to uninfected mouse lungs. Data information:  Gene expression is normalized to GAPDH expression. Y axes vary dependent upon the detected relative expression  levels after normalizing data to the internal control. (ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1 treatments, green circle uninfected mice)."
NCBI gene IDs:
"E gene: 43740570
GAPDH: 14433
ISG15: 100038882"
Figure legend: "Relative changes in gene expression for SARS-CoV-2  infections and associated modulation in inflammatory and coagulation responses are increased after   SARS-CoV-2 MA30 infection in lung  and heart  tissues (SARSCoV-2 MA30 infection - N = 18 mice  4 days follow up; N = 20 mice  7 days follow up; Uninfected mice  - N = 2). PEGSerp-1  treatment significantly modified gene expression for uPAR and complement pathways. C-L Inflammatory cytokines and growth factors are significantly increased with infection; IL-1 (C; p &lt; 0.0003), IL-6 (D p &lt; 0.0001),  IL-10 (E; p &lt; 0.0001) ), TNF  (F; p &lt; 0.0044), TGFb  (G; p &lt; 0.0022) are increased with SARS-CoV-2 infection at 4 days follow up; with VEGF  the exception (H; p = 0.4695 ) Factors in the coagulation pathway, fX  (I; p &lt; 0.0001), tPA  (J; p &lt; 0.0382), and neuroserpin  (K; p = 0.1883 ) are also increased. None of these markers are modified by PEGSerp-1 treatments. Data information: Gene expression  is normalized to GAPDH expression. Y axes vary dependent upon the detected relative expression  levels after normalizing data to the internal control. (ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1 treatments, green circle uninfected mice)."
NCBI gene IDs:
"fX: 14058
GAPDH: 14433
IL-10: 16153
IL-1: 16176
IL-6: 16193
tPA: 18791
neuroserpin: 18787
TGFb: 21803
TNF: 21926
VEGF: 22339///22340"
Figure legend: "Relative changes in  gene expression for SARS-CoV-2 infections and associated modulation in coagulation and immune responses are increased after  SARS-CoV-2 MA30 infection in lung  tissues (SARSCoV-2 MA30 infection - N = 18 mice  4 days follow up; N = 20 mice  7 days follow up; Uninfected mice  - N = 2). PEGSerp-1 treatment significantly modified gene expression  for uPAR  A-C Gene expression for  PEGserp-1 targets are increased after infection and modified by PEGSerp-1 treatments. uPA  (A; p = 0.3263), PAI-1 (B; p &lt; 0.0001) and uPAR  (C; p &lt; 0.0001) were increased in infected mouse lung  extracts at 4 days follow up when compared to uninfected lungs  with SARS-CoV-2  MA30 infection. PEG Serp-1 treatment produced a significant increase in uPAR  (C; p &lt; 0.0157). Data information: N = 19 mice  at 4 days follow up; N = 20 mice  at 7 days follow up. ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1  treatments, green circle uninfected mice)."
NCBI gene IDs:
"uPA: 5328
uPAR: 18793
PAI-1: 18787"
Figure legend: "Relative changes in gene expression for SARS-CoV-2 infections and associated modulation in coagulation and immune responses are increased after SARS-CoV-2 MA30  infection in lung  tissues (SARSCoV-2 MA30 infection - N = 18 mice  4 days follow up; N = 20 mice  7 days follow up; Uninfected mice  - N = 2). PEGSerp-1  treatment significantly modified gene expression  for complement pathways. D-F Complement pathways genes encoding C3  (D; p &lt; 0.0021), C5  (E; p &lt; 0.001) and C1INh  (F; p &lt; 0.0008) were also significantly increased with infection. PEG Serp-1 treatment produced a borderline decrease in C5  (E; p = 0.0623) and a significant increase in C1Inh  (F; p &lt; 0.0309). Data information: N = 19 mice  at 4 days follow up; N = 20 mice  at 7 days follow up. ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1 treatments, green circle uninfected mice)."
NCBI gene IDs:
"C3: 12266
C5: 15139
C1INh: 12258
C1Inh: 12258"
Figure legend: "Relative changes in  gene expression for SARS-CoV-2 infections and associated modulation in coagulation and immune responses are increased after  SARS-CoV-2 MA30 infection in lung and heart  tissues (SARSCoV-2  MA30 infection - N = 18 mice  4 days follow up; N = 20 mice  7 days follow up; Uninfected mice  - N = 2). PEGSerp-1 treatment significantly modified gene expression  for complement pathways. G-I At 7 days follow up, relative gene expression  is no longer increased for C1Inh  with PEGSerp-1  treatment in MA30  infected mouse lungs  (G; p = 0.5956), but C1Inh  was significantly increased in myocardial  extracts with PEGSerp-1 treatment at 7 days (H; p &lt; 0.0419). C5  was not significantly increased with infection (I; p = 0.1601) Data information: N = 19 mice  at 4 days follow up; N = 20 mice  at 7 days follow up. ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1 treatments, green circle uninfected mice)."
NCBI gene IDs:
"C5: 15139
C1Inh: 12258"
Figure legend: "Relative changes in gene expression for SARS-CoV-2 infections and associated modulation in coagulation and immune responses are increased after SARS-CoV-2  MA30 infection in lung  and heart  tissues (SARSCoV-2 MA30  infection - N = 18 mice  4 days follow up; N = 20 mice  7 days follow up; Uninfected mice  - N = 2). PEGSerp-1  treatment significantly modified  gene expression for uPAR  J-L PAI-1 gene expression  was not increased in lung  extracts with  PEGSerp-1 treatment at 7 days follow up in MA30  infected mouse lungs  (J; p = 0.2403), but PAI-1 expression  was significantly increased at 7 days follow up in heart  extracts (K; p &lt; 0.0454). uPAR  was not significantly increased in lung  extracts at 7 days follow up (p = 0.1514). A time course for  gene expression illustrates a peak time for changes in C1Inh gene expression demonstrates greater changes at 4 days when compared to day2 and day15 (L). Data information: N = 19 mice  at 4 days follow up; N = 20 mice  at 7 days follow up. ANOVA indicated by line at top with subgroup analyses below; infected mice  - blue circles Saline, red circle PEGSerp-1 treatments, green circle uninfected mice)."
NCBI gene IDs:
"uPAR: 18793
PAI-1: 18787
C1Inh: 12258"
Figure legend: "(A) Bar graph showing qPCR  quantification of SEC61B  level in MCF10A MECs  ligated with rBM  in 2D  and 3D 12h post-plating (mean ± SEM; n=3 independent biological replicates). Statistical analysis by one-way ANOVA followed by Uncorrected Fisher's LSD, **P = 0.0022."
NCBI gene IDs:
"SEC61B: 10952"
Figure legend: "(F) Bar graph showing qPCR of the relative level of ATF3  mRNA in MCF10A MECs  ligated with rBM  in 2D  and 3D  12h post-plating (mean ± SEM; n=3 independent biological replicates). Statistical analysis by one-way ANOVA followed by Uncorrected Fisher's LSD, **P = 0.0037."
NCBI gene IDs:
"ATF3: 467"