EMBO/SourceData huggingface dataset:
Figure legend: "(C) Quantification of area covered by cells 24 h after a scratch was made through confluent cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA. N = 5 wells/group. Right, representative images of the scratch assay."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(D,E) MDA-MB-231 cells transduced with PTPRN2, PTPRN2C945A, or control vector were subjected to the Matrigel invasion (D) and migration assays (E). N = 5 inserts/group."
NCBI gene IDs:
"PTPRN2: 5799"
Figure legend: "(F) Bioluminescence imaging quantification of lung colonization by 40,000 MDA-MB-231 cells overexpressing PTPRN2, PTPRN2C945A, or control vector. N = 5 mice/group. Right, H&amp;amp;amp;E staining of representative lung sections."
NCBI gene IDs:
"PTPRN2: 5799"
Figure legend: "(G,H) MDA-MB-231 cells transduced with PLCβ1, PLCβ1H331Q, or control vector were subjected to the Matrigel invasion (G) and migration assays (H). N = 5 inserts/group."
NCBI gene IDs:
"PLCβ1: 23236"
Figure legend: "(I) Bioluminescence imaging quantification of lung colonization by 40,000 MDA-MB-231 cells overexpressing PLCβ1, PLCβ1H331Q, or control vector. For Cntrl and PLCβ1H331Q OE, N = 6 mice/group. For PLCβ1 OE, N = 5 mice. Right, H&amp;amp;amp;E staining of representative lung sections."
NCBI gene IDs:
"PLCβ1: 23236"
Figure legend: "(C,D) MDA-MB-231 cells overexpressing PTPRN2, PLCβ1, or a control vector (C) or LM2 cells transfected with siRNA targeting PTPRN2, PLCβ1, or a control siRNA (D) were immunostained for PI(4,5)P2 levels and analyzed by fluorescence microscopy. Mean fluorescence intensity of plasma membrane levels of the lipid were quantified. N = 50 cells/group. Left, representative immunofluorescence images of cells stained with anti-PI(4,5)P2 antibody (red) and 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 10 μm."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(A,B) LM2 cells transfected with siRNA targeting PTPRN2 (A), PLCβ1 (B) or a control siRNA were transfected with Lyn11-FRB and INPP5E-FKBP, treated with either DMSO or 100 nm rapamycin and subjected to the migration assay. N = 5 inserts/group."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(C,D) MDA-MB-231 cells overexpressing PTPRN2 (C), PLCβ1 (D) or control vector were treated with carrier alone or carrier incubated with PI(4,5)P2 for one hour and then immediately subjected to the migration assay. N = 5 inserts/group."
NCBI gene IDs:
"PTPRN2: 5799"
Figure legend: "(C,D) MDA-MB-231 cells overexpressing PTPRN2 (C), PLCβ1 (D) or control vector were treated with carrier alone or carrier incubated with PI(4,5)P2 for one hour and then immediately subjected to the migration assay. N = 5 inserts/group."
NCBI gene IDs:
"PLCβ1: 23236"
Figure legend: "(E) Kaplan-Meier curve representing distal metastasis-free survival of a cohort of breast cancer patients (N = 1609) as a function of their primary tumor's mean PIP5K1A, PIP5KB, and PIP5KC expression levels (Data from KMPlot (Gyorffy et al., 2010)). Patients' primary tumors' combined PIP5K expression levels were classified as low (blue) or high (red) expression."
NCBI gene IDs:
"PIP5K1A: 8394
PIP5KB: 8395
PIP5KC: 23396"
Figure legend: "(F,G) Migration (F) and Matrigel invasion (G) of LM2 cells transduced with a retroviral vector overexpressing PIP5K1A or control vector. Data normalized to control values. N = 5 inserts/group."
NCBI gene IDs:
"PIP5K1A: 8394"
Figure legend: "(A) Kaplan-Meier curve representing distal metastasis-free survival of a cohort of breast cancer patients (N = 1609) as a function of their primary tumor's CFL1 expression levels (Data from KMPlot (Gyorffy et al., 2010)). Patients' primary tumors' CFL1 expression levels were classified as low (blue) or high (red) expression."
NCBI gene IDs:
"CFL1: 1072"
Figure legend: "(B,C) Membrane and membrane-associated proteins were purified from cells transfected with siRNA targeting PTPRN2 (B) or PLCβ1 (C) or control siRNA. Fractions were subjected to Western blot analysis for CFL1 and EGFR levels. Right, densitometry analysis of CFL1 levels normalized to EGFR levels."
NCBI gene IDs:
"PTPRN2: 5799"
Figure legend: "(B,C) Membrane and membrane-associated proteins were purified from cells transfected with siRNA targeting PTPRN2 (B) or PLCβ1 (C) or control siRNA. Fractions were subjected to Western blot analysis for CFL1 and EGFR levels. Right, densitometry analysis of CFL1 levels normalized to EGFR levels."
NCBI gene IDs:
"PLCβ1: 23236"
Figure legend: "(D,E) Membrane and membrane-associated proteins were purified from cells overexpressing PTPRN2 (D), PLCβ1 (E) or a control vector. Fractions were subjected to Western blot analysis for CFL1 and EGFR levels. Right, densitometry analysis of CFL1 levels normalized to EGFR levels."
NCBI gene IDs:
"PTPRN2: 5799"
Figure legend: "(D,E) Membrane and membrane-associated proteins were purified from cells overexpressing PTPRN2 (D), PLCβ1 (E) or a control vector. Fractions were subjected to Western blot analysis for CFL1 and EGFR levels. Right, densitometry analysis of CFL1 levels normalized to EGFR levels."
NCBI gene IDs:
"PLCβ1: 23236"
Figure legend: "(F) LM2 cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA were partially permeabilized and incubated with biotin-actin monomers. Cells were stained for incorporated biotin-actin monomers using Streptavidin-555 (red) and DAPI (blue). Right, quantification of mean fluorescence intensity of incorporated biotin-actin monomers. N = 100 cells/group. Scale bar, 20 μm."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(A) LM2 cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA were stained with phalloidin (red) and DAPI (blue) and analyzed using fluorescence microscopy. Right, mean fluorescence intensity quantification of whole cell phalloidin signal. N = 40 cells/group. Scale bar, 10 μm."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(B) Mean fluorescence intensity quantification of whole cell phalloidin signal in MDA-MB-231 cells overexpressing PTPRN2, PLCβ1, or control vector. N = 40 cells/group."
NCBI gene IDs:
"PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "(C,D) MDA-MB-231 cells were transfected with siRNAs targeting CFL1 or a control siRNA in the setting of control or PTPRN2 overexpression and subjected to the migration (C) or invasion (D) assays. N = 5 inserts/group."
NCBI gene IDs:
"CFL1: 1072
PTPRN2: 5799"
Figure legend: "(E) MDA-MB-231 cells were transfected with siRNAs targeting CFL1 or a control siRNA in the setting of control or PLCβ1 overexpression were subjected to the migration assay. N = 5 inserts/group."
NCBI gene IDs:
"CFL1: 1072
PLCβ1: 23236"
Figure legend: "(F) Bioluminescence imaging quantification of 40,000 MDA-MB-231 cells overexpressing PTPRN2 or control vector and transfected with control siRNA or siRNA targeting CFL1. Right, H&amp;amp;amp;E staining of representative lung sections. N = 5 mice/group."
NCBI gene IDs:
"CFL1: 1072
PTPRN2: 5799"
Figure legend: "(G,H) MDA-MB-231 cells overexpressing PTPRN2 (G) or PLCβ1 (H) were transfected with siRNA targeting the 3'UTR of CFL1 to deplete endogenous CFL1. Cells were further transfected with plasmids encoding either GFP-CFL1-WT or GFP-CFL1-Lck and subjected to the migration assay. N = 5 inserts/group."
NCBI gene IDs:
"CFL1: 1072
PLCβ1: 23236
PTPRN2: 5799"
Figure legend: "C Subcellular fractionation of the WT and NLS-deficient FolSrpk1-GFP  proteins. N, nucleus; C, cytosol. The amount of nuclear FolSrpk1-GFP  was set as 1. Data information: For C, each gel shown is a representative experiment carried out at least three times. The fractionation controls were Fol histone H1 (nucleus), Actin  (cytosol) and Coomassie brilliant blue (CBB) staining."
NCBI gene IDs:
"GFP: 
Srpk1: 28948139"
Figure legend: "D Quantitative measurement of H2O2  in tomato roots  after infection by the WT and FolSrpk1p::FolSrpk1-GFP  strains at 0-5 days post incubation (dpi). Data are presented as mean ± SD. The presence of different letters above the mean values of three replicates indicates a significant difference between different samples (P &lt; 0.05, ANOVA)."
NCBI gene IDs:
"GFP: 
Srpk1: 28948139"
Figure legend: "G Fluorescence microscopy analysis of FolSrpk1-GFP  in mycelia   of the FolSrpk1p::FolSrpk1-GFP  strain treated with 10 mM H2O2  for 0, 1 and 2 h. Arrows indicate the nucleus. Scale bars = 5 µm."
NCBI gene IDs:
"GFP: 
Srpk1: 28948139"
Figure legend: "I Subcellular fractionation analysis of  FolSrpk1-GFP  in mycelia  of the  FolSrpk1p::FolSrpk1-GFP  strain treated with 10 mM H2O2  for 0, 1 and 2 h. The amount of nuclear FolSrpk1-GFP  at 0 h was set as 1. Data information: For I, each gel shown is a representative experiment carried out at least three times. The fractionation controls were Fol histone H1 (nucleus), Actin  (cytosol) and Coomassie brilliant blue (CBB) staining."
NCBI gene IDs:
"GFP: 
Srpk1: 28948139"
Figure legend: "H Subcellular fractionation of FolSrpk1-GFP  and its mutant forms. The fractionation controls were Fol histone H1 (nucleus), Actin  (cytosol) and CBB staining. The amount of nuclear FolSrpk1-GFP  was set as 1. Data information: For H, , each gel shown is a representative experiment carried out at least three times."
NCBI gene IDs:
"GFP: 
Srpk1: 28948139"
Figure legend: "A K304 acetylation  (top) and amount (middle) of FolSrpk1-GFP  and FolSrpk1K304Q-GFP  in WT and FolArd1-OE strains treated with 10 mM H2O2  for 2 h. The over-expressed FolArd1-Flag  was detected by anti-Flag  (bottom). The amount of acetylated  and total FolSrpk1 in the WT was each set as 1. Data information: each gel shown is a representative experiment carried out three times."
NCBI gene IDs:
"Ard1: 28947107"
Figure legend: "E Subcellular fractionation analysis of FolSrpk1-GFP  in WT, ΔFolSir2, and FolSir2-OE strains. The amount of nuclear FolSrpk1-GFP  in the WT was set as 1. Data information: each gel shown is a representative experiment carried out three times. The fractionation controls were Fol histone H1 (nucleus), Actin  (cytosol) and CBB staining."
NCBI gene IDs:
"Sir2: 28948117"
Figure legend: "G, H Expression  profile of  FolArd1 (G) and FolSir2  (H) during the infection stage. Total RNA extracted from host roots  at 0 (conidia) and 1-5 dpi was reverse transcribed. The expression  levels were normalized to that of the Fol actin  gene. Data are presented as mean ± SD. The presence of different letters above the mean values of three replicates indicates a significant difference between different samples (P &lt; 0.05, ANOVA)."
NCBI gene IDs:
"actin: 28942483
Ard1: 28947107
Sir2: 28948117"
Figure legend: "B Secretion of the  FOXG_17180-GFP, FOXG_15294-GFP  and FOXG_13788-GFP  proteins. The proteins extracted from mycelia  (M) and culture supernatants  (S) were analyzed by  Western blotting with anti-GFP  and anti-Actin. Each gel shown is a representative experiment carried out three times. The loading controls were Fol Actin  and  CBB staining."
NCBI gene IDs:
"GFP: 
FOXG_13788: 28955025
FOXG_15294: 28956366
FOXG_17180: 28957973"
Figure legend: "F qRT-PCR analysis of Fol EF-1α transcript levels in tomato roots  14 days after infection by the indicated strains. The expression of tomato RCE1  was used as a control to ensure the use of equal amounts of RNA for  RT-PCR. Data information: For F, the presence of different letters above the mean values of four replicates indicates a significant difference between different samples (P &lt; 0.05, ANOVA). The whiskers of the boxplots indicate the upper and lower quartiles, the boxes indicate the interquartile range, and the plus sign indicates the mean."
NCBI gene IDs:
"RCE1: 
EF-1α: 28945635"
Figure legend: "F qRT-PCR analysis of Fol EF-1α transcript levels in tomato roots  14 days after infection by the indicated strains. The experiments (four biological replicates) Data information: For F, the presence of different letters above the mean values of four replicates indicates a significant difference between different samples (P &lt; 0.05, ANOVA). The whiskers of the boxplots indicate the upper and lower quartiles, the boxes indicate the interquartile range, and the plus sign indicates the mean."
NCBI gene IDs:
"EF-1α: 28945635"
Figure legend: "A Subcellular fractionation of BcSrpk1-GFP  in WT and BcSrpk1mutant strains. The amount of nuclear BcSrpk1-GFP  was set as 1. Data information: , each gel shown is a representative experiment carried out three times. The fractionation controls were histone H1 (nucleus), Actin  (cytosol) and CBB staining."
NCBI gene IDs:
"GFP: 
Srpk1: "
Figure legend: "C. BiFC assay to verify the interaction between FREE1 and CPL1. YC-FREE1 and YN-CPL1, were co-expressed with mCherry-HYL1, SE-mCherry, or DCL1-mCherry in Arabidopsis protoplasts, respectively, followed by confocal observation."
NCBI gene IDs:
"mCherry: 
DCL1: 839574
HYL1: 837498
SE: 817252"
Figure legend: "D. Co-immunoprecipitation analysis of interactions between FREE1 and CPL1 and CPL2. Arabidopsis protoplasts co-expressing mCherry-FREE1 with YFP, YFP-CPL1 or YFP-CPL2 were subjected to protein extraction and IP with GFP-trap followed by immunoblotting with mCherry and GFP antibodies."
NCBI gene IDs:
"mCherry: 
YFP: 
FREE1: 838600
CPL1: 828254
CPL2: 831743"
Figure legend: "E. Co-immunoprecipitation analysis of the associations between FREE1 and microprocessor components. Transgenic plants expressing GFP-FREE1 were subjected to protein extraction and IP with GFP-trap followed by immunoblotting with indicated antibodies."
NCBI gene IDs:
"GFP: 
FREE1: 838600"
Figure legend: "B. Small RNA gel blots for 7-day-old seedlings Col-0 and free1-ctmut plants. U6 was used as a loading control. The numbers indicate the relative abundance of miR168, miR166, miR162, siRNA1003, tasiRNA255, IR71, miR156 and miR172 in the plants (the intensity in WT was arbitrarily annotated as 1.00)."
NCBI gene IDs:
"IR71: 
siRNA1003: 
tasiRNA255: 
free1: 838600
miR156: 28721198///3770614///28721147///28720200///28720191///28718303
miR162: 28721190///28720955
miR166: 3771553///3771422///3771398///3770612///3770611///3769765///28718357
miR168: 28721252///28720148
miR172: 5008315///5008089///5007991///3770659///28718310
U6: 28719273"
Figure legend: "A. Detection of HYL1 phosphorylation in Col-0, free1-ctmut or cpl1-8. Proteins extracted from 7-day-old Arabidopsis seedlings were separated by Phos-tag polyacrylamide gels or SDS polyacrylamide gels followed by immunoblotting with indicated antibodies. The numbers in the first row indicate the relative grayscale intensities of total HYL1, the numbers in the second row indicate the relative intensities of dephosphorylated HYL1 and phosphorylated HYL1, and the intensity in WT was arbitrarily set to 1.00."
NCBI gene IDs:
"free1: 838600
cpl1: 828254"
Figure legend: "B. Transgenic plants expressing pHYL1:: YFP-HYL1 in Col-0, free1-ctmut or cpl1-8 backgrounds were subjected to protein extraction and IP with GFP-trap, followed by detection with phos-tag BTL-111 or GFP antibody. The numbers indicate the relative intensities of each band detected with phos-tag BTL-111 and anti-GFP, and the first lane was arbitrarily set to 1.00."
NCBI gene IDs:
"YFP: 
free1: 838600
cpl1: 828254
HYL1: 837498"
Figure legend: "C. Confocal observation of the YFP-HYL1-containing D-bodies in Col-0 and free1-ctmut roots. Arrows indicated the YFP-HYL1-containing D-bodies. D. Statistically analysis of the number of YFP-HYL1-containing D-bodies as shown in Fig 4C. The X-axis represents the number of D-bodies per cell, and the Y-axis represents the percentage of cells with the corresponding numbers. The quantification was performed by observing more than 400 cells from 20 roots for each genotype"
NCBI gene IDs:
"free1: 838600"
Figure legend: "E. Protein abundance of core components of miRNA processing complex in Col-0 and free1-ctmut. Proteins extracted from 7-day-old Arabidopsis seedlings were used for immunoblotting with indicated antibodies. The numbers represent the intensities of each band detected with indicated antibody relative to anti-UGPase, and the first lane was arbitrarily set to 1.00."
NCBI gene IDs:
"free1: 838600"
Figure legend: "A. Proteins extracted from Arabidopsis protoplasts co-expressing YFP-CPL1 and different amount of 3myc-FREE1 were used for immunoprecipitation with GFP-Trap followed by detection with indicated antibodies."
NCBI gene IDs:
"3myc: 
YFP: 
FREE1: 838600
CPL1: 828254"
Figure legend: "B. Proteins extracted from transgenic plants expressing YFP-CPL1 in Col-0 or free1-ctmut background were used for IP with GFP-trap followed by immunoblotting analysis with indicated antibodies."
NCBI gene IDs:
"YFP: 
free1: 838600
CPL1: 828254"
Figure legend: "C. Statistically analysis of the relative intensity of FREE1, HYL1 and SE in Col-0 or free1-ctmut background (IP/Input). Data are presented as means ± s.d. (3 biological replicates), the asterisk indicates significant difference between the samples (**P &lt; 0.01 by Student's t-test, compared with WT)."
NCBI gene IDs:
"free1: 838600"
Figure legend: "D. Transgenic plants of YFP-HYL1 / WT, YFP-HYL1 / free1-ctmut, YFP-HYL1 / cpl1-8 and GFP / WT were subjected to protein extraction and IP with GFP-trap followed by immunoblotting analysis with indicated antibodies."
NCBI gene IDs:
"YFP: 
free1: 838600
cpl1: 828254
HYL1: 837498"
Figure legend: "Sample recordings from 7-week-old  hESC-derived Cerebral Organoids (COs). Sample traces show visible differences in local field potential, with WWOX-KO  COs (Red) showing increased activity compared to WT (Blue) in baseline condition (Left) and in the presence of 100µM 4AP (Right)."
NCBI gene IDs:
"WWOX: 51741"
Figure legend: "Sample recordings from 7-week-old hESC-derived  Cerebral Organoids (COs). WWOX's coding sequence was re-introduced into week 6 WWOX-KO  COs using lentiviral transduction (lenti-WWOX) (F) Immunofluorescent staining  showing WWOX expression in different populations in WWOX-KO  organoids following infection with  lentivirus. NT= non-treated. Scale = 50µm ."
NCBI gene IDs:
"WWOX: 51741"
Figure legend: "(A) Fluorescence profiles of mNG-PilG  in chpA  mutants after 2h on surfaces. Solid lines, mean normalized fluorescence profiles across biological replicates. Shaded area, standard deviation across biological replicates."
NCBI gene IDs:
"chpA: 878176"
Figure legend: "(D) Polar localization  profiles of mNG-PilG  in ΔfimL  and ΔfimL  ΔchpA mutant after 2h on surfaces."
NCBI gene IDs:
"chpA: 878176
fimL: 881776"
Figure legend: "(A) mNG-PilH  fluorescence profiles in chpA  mutants after 2h surface growth. To avoid negative effects of low cAMP  level on localization  of PilH, cpdA  was deleted in all displayed strains to rescue cAMP  level to WT levels Solid lines, mean normalized fluorescence profiles across biological replicates. Shaded area, standard deviation across biological replicates."
NCBI gene IDs:
"chpA: 878176
cpdA: 880028"
Figure legend: "C) Fluorescence profiles of mNG-PilHGOF after 2h on surface in chpA  mutants. C) Solid lines, mean normalized fluorescence profiles across biological replicates. Shaded area, standard deviation across biological replicates."
NCBI gene IDs:
"chpA: 878176"
Figure legend: "(A) mNG-PilH  fluorescence profiles in ΔpilG after 2h surface growth (low cAMP)."
NCBI gene IDs:
"pilG: 878203"
Figure legend: "(C) mNG-PilH  fluorescence profiles in ΔpilG ΔcpdA  after 2h surface growth (rescued cAMP)."
NCBI gene IDs:
"cpdA: 880028
pilG: 878203"
Figure legend: "(A) mNG-PilG  fluorescence profiles in pilH  mutants after 2h surface growth. We included ΔcpdA for comparison since both pilH  mutations result in high cAMP  levels. PilG  is more polar and asymmetric in ΔpilH  and pilHLOF. (A Solid lines, mean normalized fluorescence profiles across biological replicates. Shaded area, standard deviation across biological replicates."
NCBI gene IDs:
"cpdA: 880028
pilH: 878198"
Figure legend: "(D) Time course of PilG localization  in pilHGOF on surface. For clarity only the 2h profile of PilG  in wild type was included. D) Solid lines, mean normalized fluorescence profiles across biological replicates. Shaded area, standard deviation across biological replicates."
NCBI gene IDs:
"pilH: 878198"
Figure legend: "(A, Time course  fluorescence profiles and polar localization  indexes of mNG-PilG  in ΔpilA. PilG  polar localization  index remains unchanged on surfaces in ΔpilA  similar to surface-adapted WT"
NCBI gene IDs:
"pilA: 878423"
Figure legend: "(C Time course fluorescence profiles and polar localization  indexes of mNG-PilH  in ΔpilA. Like in WT, PilH  gets recruited to the poles over time  in ΔpilA, although at a slower speed"
NCBI gene IDs:
"pilA: 878423"
Figure legend: "B. T6SS secretion assay of A. tumefaciens strains: wild type C58, various mutants lacking one, two, or three toxin-immunity gene pairs, and a mutant lacking tssL."
NCBI gene IDs:
"tssL: 1136207"
Figure legend: "C. T6SS secretion assay of various A. tumefaciens strains: wild type C58, ΔtssL, the tde double deletion mutant (Δtdei) containing pRL662 and pTrc200 empty vector (V) only or expression of pTdei1 (tde1-tdi1 expressed from pTrc200), pTde2* (catalytic site mutated tde2 expressed on pRL662), or pTdei1+ pTde2*."
NCBI gene IDs:
"Tde2: 1135514
tde2: 1135514
tde1: 1136224
tdi1: 1136225
tssL: 1136207"
Figure legend: "A. T6SS secretion assay of A. tumefaciens strains: wild type C58, ΔtssL, Δtap-1, and Δatu3641 harboring a pTrc200 vector (V) or derivatives pTap-1 (tap-1 expressed from pTrc200), or p3641 (atu3641 expressed from pTrc200)."
NCBI gene IDs:
"atu3641: 1135515
tap-1: 1136223
Tap-1: 1136223
tssL: 1136207"
Figure legend: "B. T6SS secretion assay of A. tumefaciens C58 Δtap-1Δatu3641 containing an empty pTrc200 vector (V) or its derivatives expressing tap-1, atu3641, tde1-tdi1, or tde2-tdi2."
NCBI gene IDs:
"tdi2: 1135513
tde2: 1135514
atu3641: 1135515
tap-1: 1136223
tde1: 1136224
tdi1: 1136225"
Figure legend: "A. Representative images of cells of A. tumefaciens C58 strains ΔtssB, ΔtssLΔtssB, and ΔtdeiΔtssB cells each expressing TssB-GFP from pTrc200. Upper panel: phase contrast images; Lower panel: green fluorescence images. Examples of GFP streaks were indicated by white arrowheads. Scale bar: 2 μm."
NCBI gene IDs:
"tssB: 1136216
tssL: 1136207"
Figure legend: "C. Western blots of the isolated sheaths, which were prepared via ultracentrifugation of cell lysates from A. tumefaciens C58 wild type, ΔtssL, and Δtdei. T: total proteins from the cell lysate. P: pellet samples containing TssBC sheaths after ultracentrifugation. S: supernatant after ultracentrifugation. Proteins were analyzed in western blots probed with indicated antibodies. Fha serves as a cytoplasmic control. Molecular weight markers (in kDa) are indicated on the left. Similar results were obtained from at least two independent experiments. Data are from one independent experiment and reproduced in at least two independent experiments."
NCBI gene IDs:
"tssL: 1136207"
Figure legend: "D. Secretion assay of wild type 12D1, ΔtssL, Δv1, Δv3-4 containing pRL662 empty vector (V) or its derivatives with v1 or v3-4 were analyzed for secretion."
NCBI gene IDs:
"tssL: 
v1: 
v3: "
Figure legend: "E. Secretion assay of wild type 1D1108, ΔtssL, Δv4, Δv6 containing pRL662 empty vector (V) or its derivatives with v4 or v6."
NCBI gene IDs:
"v4: 
v6: 
tssL: 1136207"
Figure legend: "A. T6SS secretion assay of A. tumefaciens wild type C58, ΔtssL, and various single, double, and triple toxin-immunity deletion strains."
NCBI gene IDs:
"tssL: 1136207"
Figure legend: "B. T6SS secretion assay of A. tumefaciens wild type C58, ΔtssL, Δtdei, and Δ3TIs harboring the indicated plasmids."
NCBI gene IDs:
"tssL: 1136207"
Figure legend: "A. T6SS secretion assay of A. tumefaciens 12D1 wild type, ΔtssL, and various toxin-immunity deletion strains."
NCBI gene IDs:
"tssL: "
Figure legend: "B. T6SS secretion assay of A. tumefaciens 12D1 wild type, ΔtssL, and various toxin-immunity deletion strains harboring the indicated plasmids."
NCBI gene IDs:
"tssL: "