EMBO/SourceData huggingface dataset:
Figure legend: "G HeLa  cells were transfected with GFP-PIKfyve. After 24 hours, cells were were treated with 5 µM YM201636  for 2 hours, then YM201636  was washed out for 1 hour. Cells were fixed and immunostained  with SNX2, GFP  and LAMP2  antibodies, and imaged using super resolution microscopy. Arrows show  co-localization  between GFP-PIKfyve, SNX2  and LAMP2. Data information: The insets at the lower right or bottom of each image are higher power regions of the boxed areas. Scale bar is 10 µm in"
NCBI gene IDs:
"GFP: 
PIKfyve: 200576"
Figure legend: "H  MCF-7 cells expressing LAMP1-mCherry   were transfected with NT or SNX2  siRNA. After 24 hours, cells were imaged by spinning disk microscopy for 20 minutes. Representative timelapse  snapshots of LAMP1-mCherry  from a maximum projection of 3 z-planes taken 0.27 µm apart are shown. Arrow indicates lysosome fission. I Data represent the number of LAMP1+ reformation events per cell per minute (n=21 NT siRNA cells, n=27 SNX2  siRNA cells). Data information: Data is presented as mean ± SD. The insets at the lower right or bottom of each image are higher power regions of the boxed areas. Scale bar is 10 µm in H, p values determined by two-tailed unpaired t test in I."
NCBI gene IDs:
"mCherry: 
LAMP1: 25328
SNX2: 6643"
Figure legend: "A, B HeLa   cells transfected with INPP4B  or NT siRNA were treated for 1 hour with 5 µg/mL puromycin. Cells were fixed and immunostained  with ubiquitin  antibodies, and co-stained  with DAPI  and phalloidin (A). Data represent the percentage of cells with ubiquitinated  protein aggregates (n=3 experiments, &gt;200 cells/experiment) (B). Data information: Data is presented as mean ± SD. The insets at the lower right or bottom of each image are higher power regions of the boxed areas. Scale bar is 10 µm in A p values determined by one-way ANOVA in with Tukey post hoc test in B"
NCBI gene IDs:
"INPP4B: 8821"
Figure legend: "E, F MCF-7  cells expressing GFP-INPP4B  or GFP-vector were treated for 4 hours with 10 µg/mL puromycin, 5 µm MG132  and either 5 µM YM201636  or DMSO  as a vehicle control. Cells were fixed and immunostained  with ubiquitin  antibodies, and co-stained with DAPI  and phalloidin  (E). Data represent the percentage of cells with ubiquitinated  protein aggregates (n=3 experiments, &gt;200 cells/experiment) (F). Data information: Data is presented as mean ± SD. The insets at the lower right or bottom of each image are higher power regions of the boxed areas. Scale bar is 10 µm in E. p values determined by one-way ANOVA in with Tukey post hoc test in"
NCBI gene IDs:
"GFP: 
INPP4B: 8821"
Figure legend: "G HeLa  cells transfected with INPP4B  or NT siRNA were treated with 10 µg/mL puromycin  for 2-6 hours, then cell viability was assessed using CellTiter-Glo® assays. Data represent the relative  cell viability normalized to untreated NT siRNA cells (n=3 experiments). Data information: p values determined by two-tailed unpaired t test of the area under the curve in G"
NCBI gene IDs:
"INPP4B: 8821"
Figure legend: "a, Wild-type (WT) and Park2-/- BMDMs were infected with mCherry-expressing M. tuberculosis (Mtb) for 4 h and immunostained using anti-parkin antibodies."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "d, U937 human macrophages expressing a scrambled shRNA (Control) or one of two different shRNAs targeting PARK2 (shRNA no. 1, shRNA no. 2) were infected with mCherry-expressing M. tuberculosis for 12 h and immunostained for polyubiquitin."
NCBI gene IDs:
"PARK2: 5071"
Figure legend: "d, U937 human macrophages expressing a scrambled shRNA (Control) or one of two different shRNAs targeting PARK2 (shRNA no. 1, shRNA no. 2) were infected with mCherry-expressing M. tuberculosis for 12 h and immunostained for polyubiquitin. e, Quantification of ubiquitin positive M. tuberculosis from d, results are means ± s.e.m. of three independent experiments (**P  0.005, Student's t-test). f, The parkin and actin expression in cells from e was determined by western blotting."
NCBI gene IDs:
"PARK2: 5071"
Figure legend: "g, Park2−/− BMDMs were transduced with lentivirus expressing BFP (−), wild-type parkin, or two separate mutant parkin alleles (T240R, P437L). Cells were infected with M. tuberculosis for 4 h and ubiquitin and M. tuberculosis co-localization was quantified and expressed relative to control BMDMs. Results are means ± s.e.m. of three independent experiments (**P  0.005, paired Student's t-test). h, The parkin and actin expression in cells from g was determined by western blotting."
NCBI gene IDs:
"Park2: 50873
parkin: 50873"
Figure legend: "g, RAW264.7 macrophages transduced with lentivirus expressing green fluorescent protein (GFP) or parkin were infected with M. tuberculosis and c.f.u. were determined after 16 h. Results were normalized to GFP-expressing cells (n = 3 per group, **P &lt; 0.0076)."
NCBI gene IDs:
"parkin: 50873"
Figure legend: "h, U937 human macrophages expressing either scrambled or PARK2 shRNAs were infected with M. tuberculosis for 36 h and c.f.u were determined. Results were normalized to t = 0 (N = 3 per group, *P&lt; 0.02). All errors, s.e.m."
NCBI gene IDs:
"PARK2: 5071"
Figure legend: "a, WT and Park2-/- mice were infected with wild-type M. tuberculosis by aerosol and lung bacterial burdens were determined by plating (means ± s.d., n = 5 per group, *P &lt; 0.02 by Student's t-test)."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "c, Survival of M. tuberculosis-infected wild-type and Park2-/- mice (n = 10, **P &lt; 0.001 by log-rank test)."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "a, Wild-type and two independent parkin-deficient D. melanogaster lines (parkc00062, parkf01950) were infected with L. monocytogenes by anterior abdomen injection. Atg8 processing was monitored in whole-fly protein lysates by western blotting."
NCBI gene IDs:
"parkin: 40336"
Figure legend: "Single plane confocal fluorescent microscopy images of 5-day-old animals harboring GFP-tagged endogenous HSP-110 (HSP-110::GFP). Muscle cells are outlined. M: muscle, H: hypodermis. In animals that co-express hairpin constructs targeting hsp-110 in muscle cells (HSP-110;HPI and HSP-110;HPII), GFP fluorescence is strongly reduced in muscle cells in comparison to control animals, indicating HSP-110::GFP depletion specifically in muscle cells. Scale bar: 10 µm."
NCBI gene IDs:
"GFP: 
hsp-110: 176195"
Figure legend: "Experimental set-up: Age-synchronized 4-day-old animals were subjected to heat stress for 3 h at 33°C (+HS) then returned to 20°C or left at 20°C (-HS), respectively. Arrows indicate imaging time points. The -HS controls were imaged at the same time point as the +HS animals after 24h at 20°C. Maximum intensity projections of fluorescent microscopy z-stacks of animals expressing the indicated transgenes. HS-induced FLUCSM::GFP foci are cleared after a recovery for 24h at 20°C in the WT background, but persist in the presence of the hsp-110 hairpin construct. Scale bar: 20 µm. C Quantification of FLUCSM::GFP foci disaggregation. % disaggregation is calculated as 100 - ratio of FLUCSM::GFP foci area relative to total muscle area at the +HS 24h time point to the +HS 2h time point. Data are displayed as mean ± SEM (in %) (N = 3). Statistical analysis was done using one-way ANOVA with Dunnett's adjustment for multiple comparisons. ** p ≤ 0.01. Data information: All strains harbor the sid-1(pk3321) allele. "
NCBI gene IDs:
"hsp-110: 176195
sid-1: 178900"
Figure legend: "A Maximum intensity projections of fluorescent microscopy z-stacks of 5-day-old nematodes expressing the indicated transgenes are shown. White dashed lines outline the borders of muscle cells. Scale bar: 10 µm. The co-expression of HPI and HPII reduces the amount of α-Syn::YFP inclusions. B Quantification of α-Syn::YFP foci in 3- to 6-day-old animals. Product of mean foci fluorescence and foci area relative to muscle area is displayed (relFluoFoci). Data are shown as mean ± SEM (in %) (N = 3). Statistical analysis was done using two-way ANOVA with Dunnett's multiple comparison test. ** = p ≤ 0.01, *** p ≤ 0.001. "
NCBI gene IDs:
"α-Syn: 6622"
Figure legend: "D Maximum intensity projections of fluorescent microscopy z-stacks of 6-day-old animals expressing the indicated transgenes. Scale bar: 100 µm. The co-expression of HPI and HPII reduces the amount of Q35::YFP inclusions."
NCBI gene IDs:
"Q35: "
Figure legend: "E Quantification of Q35::YFP foci in WT and hsp-110 hairpin background. Shown is the mean ± SEM (N = 3). Statistical analysis was done using two-way ANOVA with Dunnett's multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01."
NCBI gene IDs:
"hsp-110: 176195"
Figure legend: "G Maximum intensity projections of fluorescent microscopy z-stacks of of 5-day-old nematodes expressing the indicated transgenes. White dashed lines outline the borders of muscle cells. Scale bar: 10 µm. M: muscle, H: hypodermis. Signal outside of muscle cells reveals spreading of α-Syn. H Quantification of animals showing α-Syn transmission at indicated ages. Displayed is the mean ± SEM (in %) (N = 4). Statistical analysis was done using two-way ANOVA with Dunnett's multiple comparison test. *** p ≤ 0.001. "
NCBI gene IDs:
"α-Syn: 6622"
Figure legend: "A Maximum intensity projections of confocal microscopy z-stacks of 5-day-old animals expressing the indicated transgenes. White dashed lines outline the borders of muscle cells. Scale bar: 10 µm. The expression of the yeast SSE1N575Y E575A mutant cannot reverse the reduction of α-Syn::YFP foci in the hsp-110 hairpin expressing animals."
NCBI gene IDs:
"hsp-110: 176195
SSE1: 855998"
Figure legend: "B Quantification of motility as a measure for transgene toxicity. Displayed is the mean ± SEM (N = 3) amount of body bends/30s of animals expressing the indicated transgenes. Statistical analysis was done using two-way ANOVA and with Dunnett's multiple comparison test. n.s. = not significant, ** p ≤ 0.01, *** p ≤ 0.001. The presence of the yeast SSE1N575Y E575A mutant was not able to suppress the rescue of α-Syn::YFP toxicity in hsp-110 hairpin expressing animals."
NCBI gene IDs:
"hsp-110: 176195
SSE1: 855998"
Figure legend: "E Experimental set-up: Age-synchronized 4-day-old animals were subjected to heat stress for 3 h at 33°C (+HS) then returned to 20°C or left at 20°C (-HS), respectively. Arrows indicate imaging time points. The -HS controls were imaged at the same time point as the +HS animals after 24h at 20°C. Maximum intensity projections of fluorescent microscopy z-stacks of animals expressing the indicated transgenes. Scale bar: 20 µm. The impaired clearance of HS-induced FLUCSM::GFP foci in the hsp-110 hairpin expressing cells is rescued by co-expression of WT but not by the HSP-70 binding deficient SSE1N575Y E575A mutant."
NCBI gene IDs:
"hsp-110: 176195
SSE1: 855998"
Figure legend: "F Quantification of FLUCSM::GFP foci disaggregation. % disaggregation is calculated as 100 - ratio of FLUCSM::GFP foci area relative to total muscle area at the +HS 24h time point to the +HS 2h time point. Data are displayed as mean ± SEM (in %) (N = 3). Statistical analysis was done using one-way ANOVA with Dunnett's multiple comparison test. n.s. = not significant, ** p ≤ 0.01. All strains harbor the sid-1(pk3321) allele. "
NCBI gene IDs:
"sid-1: 178900"
Figure legend: "A Maximum intensity projections of confocal z-stacks of 4-day-old animals expressing the indicated transgenes or harboring the indicated temperature-sensitive (ts) mutations. Muscle cells were stained with anti-myosin (green) and Alexa Flour 647-phalloidin (purple). Scale bar: 5 µm. Myosin misfolding reflects the exposure of the ts mutant phenotype of myosin(ts) [unc-54(e1301)] at the permissive temperate of 15°C by co-expression of the hsp-110 hairpins."
NCBI gene IDs:
"hsp-110: 176195"
Figure legend: "B Quantification of motility as a measure for toxicity. Displayed is the mean ± SEM (N = 4) of body bends/30s of animals expressing the indicated transgenes or harboring the indicated mutations. Statistical analysis was done using one-way ANOVA with Dunnett's multiple comparison test. *** p ≤ 0.001. Co-expression of the hsp-110 hairpins exposed the ts mutant phenotype at the permissive temperate of 15°C, resulting in a significant increase in movement defects. All strains contain the sid-1(pk3321) allele."
NCBI gene IDs:
"hsp-110: 176195
sid-1: 178900"
Figure legend: "C Quantification of motility as a measure for transgene toxicity. Displayed is the mean ± SEM (N = 4) amount of body bends/30s of animals expressing the indicated transgenes or harboring the indicated mutations. Statistical analysis was done using two-way ANOVA with Tukey's multiple comparison test. * p ≤ 0.05. Expression of the hsp-110 hairpins caused movement defects with increasing age."
NCBI gene IDs:
"hsp-110: 176195"
Figure legend: "D Maximum intensity projections of confocal z-stacks of 12-day-old animals expressing the indicated transgenes. Scale bar: 10 µm. FLUCSM forms foci with increasing age, which is markedly accelerated upon hsp-110 KD."
NCBI gene IDs:
"hsp-110: 176195"
Figure legend: "F Quantification of motility as a measure for transgene toxicity. Displayed is the mean ± SEM (N = 3) amount of body bends/30s of animals expressing the indicated transgenes or harboring the indicated mutations. Statistical analysis was done using two-way ANOVA with Dunnett's multiple comparison test. n.s. = not significant, *** p ≤ 0.001. Expression of the hsp-110 hairpins caused movement defects with increasing age. The beneficial effects of HSP-110 KD on amyloid toxicity is masked in older animals."
NCBI gene IDs:
"hsp-110: 176195
HSP-110: 176195"
Figure legend: "G Quantification of motility as a measure for transgene toxicity. Displayed is the mean ± SEM (N = 3) amount of body bends/30s of animals expressing the indicated transgenes or harboring the indicated mutations. Statistical analysis was done using two-way ANOVA with Dunnett's multiple comparison test. n.s. = not significant, , ** p ≤ 0.01, *** p ≤ 0.001. Expression of the hsp-110 hairpins caused movement defects with increasing age. The beneficial effects of HSP-110 KD on amyloid toxicity is masked in older animals. Data information: All strains harbor the sid-1(pk3321) allele. "
NCBI gene IDs:
"hsp-110: 176195
HSP-110: 176195
sid-1: 178900"
Figure legend: "C Representative electron micrographs of VAP double knockout HeLa cells that were either left untreated or incubated with 250 µM LLOMe for 5 min or 10 min before chemical fixation. Cells were incubated with 15 nm gold nanoparticles conjugated to bovine serum albumin for 4 h to visualize lysosomes. After 4 h, gold nanoparticles were washed off and cells were incubated overnight before treatment with LLOMe and processing samples for electron microscopy (same as in A). Quantification of electron micrographs showing no increase in membrane contact sites between ER and lysosomes following 250 µM LLOMe treatment for 5 or 10 minutes in VAP double knockout cells. Error bars denote +/- SD from n=3 independent experiments, &gt;40 lysosomes were quantified per experiment for each condition."
NCBI gene IDs:
"VAP: 9217///9218"
Figure legend: "B HeLa cells were transfected with control siRNA or siRNA targeting PI4K2A or PI4K2B for 48 h and transiently transfected with the 2xSidM-eGFP probe to monitor PtdIns4P recruitment after LLOMe-treatment by live-cell imaging. Representative movie stills show the dynamics of 2xSidM-eGFP recruitment in control, PI4K2A and PI4K2B depleted cells. The graph represents the quantification of 2xSidM-eGFP foci per cell after LLOMe-treatment. Error bars denote +/- SEM from n=3 independent live-cell imaging experiments, &gt;25 cells were analyzed per experiment for each condition."
NCBI gene IDs:
"PI4K2A: 55361
PI4K2B: 55300"
Figure legend: "C CRISPR-Cas9 mediated knockout of PI4K2A abolishes recruitment of the 2xSidM-eGFP probe (PtdIns4P) to damaged lysosomes. Two different clones (clone 2 and clone 3) were tested. The graph represents the quantification of 2xSidM-eGFP foci per cell after LLOMe-treatment. Error bars denote +/- SD from n=2 independent live-cell imaging experiments, &gt;20 cells were analyzed per experiment for each condition."
NCBI gene IDs:
"CRISPR: 
Cas9: 69900935
PI4K2A: 55361"
Figure legend: "D Representative movie stills from live fluorescence microscopy showing reduced eGFP-ORP1L recruitment in PI4K2A knockout cells compared to wild type HeLa cells upon treatment with 250 µM LLOMe. The graph represents the quantification of eGFP-ORP1L foci per cell after LLOMe treatment. Error bars denote +/- SEM from n=4 independent live-cell imaging experiments, &gt;80 cells were analyzed per experiment for each condition."
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "Representative movie stills from live-cell imaging experiments monitoring Lysotracker recovery after induced lysosomal damage using 250 µM LLOMe. Cells were pre-treated with 75 nM Lysotracker Deep Red for 30 min before addition of LLOMe. The graph represents the quantification of bright Lysotracker positive foci per cell. While the decrease in the number of Lysotracker spots is quickly recovering in HeLa cells, there is a severe impairment in lysosomal repair in HeLa-PI4K2A-KO cells."
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "A HeLa cells were co-transfected with siRNAs targeting ESCRT proteins TSG101 and ALIX (siTSG101+siALIX) or with non-targeting siRNA control (siControl), and incubated for 24 h before transfection with the PtdIns4P binding probe 2xSidM-eGFP. 24 h post-transfection lysosomes were damaged with 250 µM LLOMe and PtdIns4P recruitment to damaged lysosomes was monitored using the 2xSidM-eGFP probe in live-cell imaging experiments. The graph shows quantification of 2xSidM-eGFP foci per cell in cells co-depleted of TSG101+ALIX and control (siControl) cells."
NCBI gene IDs:
"ALIX: 10015
TSG101: 7251"
Figure legend: "D HeLa cells expressing an eGFP-tagged ORP1L mutant incapable of binding to VAP (mFFAT) show no accumulation of the cholesterol reporter mCherry-D4 upon lysosomal damage induced with 250 µM LLOMe when compared to wildtype (wt) eGFP-ORP1L. The quantification graph shows D4-mCherry foci per cell. Error bars denote +/- SEM from n=4 independent live-cell imaging experiments, &gt;50 cells were analyzed per experiment for each condition."
NCBI gene IDs:
"eGFP: 
ORP1L: 114876
VAP: 9217///9218"
Figure legend: "D Knock down efficiency of siRNA depletion of OSBP in HeLa cells as detected by Western blot. β-actin used as a loading control."
NCBI gene IDs:
"OSBP: 5007"
Figure legend: "Conditioned medium was collected from pericytes 48 h after adenoviral transduction. Western blotting verified the secretion of Sema3CΔ13 (81 kDa) and Sema3Cp60 (60 kDa) isoforms."
NCBI gene IDs:
"Sema3C: 10512"
Figure legend: "HUVECs were suspended in conditioned medium with VEGF (25 ng/ml) and plated on Matrigel. Branch points of the capillary-like network were counted after 18 h. n = 4 independent experiments; scale bar, 200 μm. Control vs. Sema3C, *P = 0.0375 and control vs. Sema3CΔ13, *P = 0.0162."
NCBI gene IDs:
"Sema3C: 10512"
Figure legend: "HUVEC spheroids in collagen were treated with conditioned medium with or without VEGF (25 ng/ml). After 24 h, the cumulative sprout length per spheroid was quantified. Data are representative of three independent experiments with ten spheroids per group. Scale bar, 100 μm. Control vs. Sema3C (− VEGF), *P = 0.0276; control vs. Sema3CΔ13 (− VEGF), *P = 0.0443; control vs. Sema3C (+ VEGF), *P = 0.028 and control vs. Sema3CΔ13 (+ VEGF), *P = 0.032."
NCBI gene IDs:
"Sema3C: 10512"
Figure legend: "Microvessels within the plugs stained against human-specific CD34. Cell nuclei were stained with DAPI. Scale bar, 100 μm.The area of CD34-positive microvessels was quantified and normalized to the total area of plug. Results represent the means ± s.e.m of 5 plugs (control) and 7 plugs (Sema3C), *P = 0.018."
NCBI gene IDs:
"Sema3C: 10512"
Figure legend: "Vessel coverage was assessed by αSMA staining. Scale bar, 100 μm.Vessel coverage was quantified as the ratio of endothelial cell-associated mural cells to total amount of vessels (n = 5 (control) and n = 7 (Sema3C)).Vessel perfusion was evaluated by i.v. injection of FITC-lectin (0.15 mg) 30 min before mice were sacrificed. The data show the percentage of perfused vessels in the plug and normalized to total vessel area (n = 5 (control) and n = 7 (Sema3C))."
NCBI gene IDs:
"Sema3C: 10512"
Figure legend: "HUVECs were transfected with siRNA against PlexinD1 or control NS (non-silence) siRNA. qPCR analyses and Western blotting were performed after 48 h (n = 3; paired Student's t-test, NS siRNA vs. PlexinD1 siRNA, ***P = 0.0004). Cell lysates were subjected to SDS-PAGE and probed with anti-PlexinD1."
NCBI gene IDs:
"PlexinD1: 23129"
Figure legend: "HUVECs were transfected with siRNA against PlexinD1 and plated on gelatin-coated coverslips. Confluent monolayers were treated with Sema3C-, Sema3Cp60- or control-conditioned medium for 30 min. Cells were fixed and stained with Alexa-Fluor 488-conjugated phalloidin to visualize F-actin. Arrows indicate Sema3C-induced cell membrane ruffling. Scale bar, 20 μm. Cell repellence was quantified as the number of cells with membrane ruffling normalized to the number of nuclei (n = 5; mean ± s.e.m., paired Student's t-test, **P = 0.001)."
NCBI gene IDs:
"PlexinD1: 23129"
Figure legend: "Silencing of Nrp-1 by lentiviral particles expressing shRNA. After culturing 72 h in selection medium, RNA was harvested for qPCR analysis. NS shRNA vs. Nrp-1 shRNA: #1, *P = 0.017 and #2, *P = 0.02; n = 3; mean ± s.e.m. Cell lysates were subjected to SDS-PAGE and probed with anti-Nrp-1."
NCBI gene IDs:
"Nrp-1: 8829"
Figure legend: "Depletion of Nrp-1 abolished the response to Sema3C. Sema3Cp60 had no effect on HUVEC adhesion. Arrows indicate membrane ruffling. Scale bar, 20 μm. Cell repellence was quantified as the number of cells with membrane ruffling normalized to the number of nuclei (n = 5; mean ± s.e.m.; paired Student's t-test, ***P = 0.0003)."
NCBI gene IDs:
"Nrp-1: 8829"
Figure legend: "RNA scope analyses showed Sema3C and PlexinD1 expression in pre-retinal tufts (OIR model, P19). The retinae were subsequently stained against collagen IV. Sema3CmRNA was detected in peri-endothelial cells. Scale bar: 20 μm."
NCBI gene IDs:
"PlexinD1: 67784
Sema3C: 20348"
Figure legend: "H. Subcellular localizations of zRbm14b resembled those of zRbm14a. Zebrafish embryos were microinjected with 400 pg of GFP-Rbm14b mRNA per egg at the 1-cell stage and fixed at 4 hpf."
NCBI gene IDs:
"GFP: 
Rbm14b: 402858"
Figure legend: "Phenotypes of zRbm14 morphants. Totally 8 ng of ctrl-MO, 14-tMOs, or 14-MOs were co-injected with 100 pg of in-vitro transcribed GFP mRNA (as a tracer) into 1-cell embryos to generate control, maternal zRbm14, and zygotic zRbm14 morphants, respectively. Representative embryos, imaged under a dissecting microscope, are shown in (A)"
NCBI gene IDs:
"GFP: 
Rbm14: 402858///323721"
Figure legend: "Phenotypes of zRbm14 morphants. Totally 8 ng of ctrl-MO, 14-tMOs, or 14-MOs were co-injected with 100 pg of in-vitro transcribed GFP mRNA (as a tracer) into 1-cell embryos to generate control, maternal zRbm14, and zygotic zRbm14 morphants, respectively. phenotyping results in (B). Total numbers of embryos analyzed are listed over histograms. Sample dots are included for quantification results with no error bars."
NCBI gene IDs:
"GFP: 
Rbm14: 402858///323721"
Figure legend: "Maternal zRbm14 morphants displayed reduced cell proliferation in MZT. Embryos stained with Hoechst 33342 and Pyronin Y to mark the nucleus and the cytoplasm, respectively, were imaged from the animal pole to cover a 10-μm z-depth with a confocal microscope (C). Maximum intensity-projected images (C)"
NCBI gene IDs:
"Rbm14: 402858///323721"
Figure legend: "Maternal zRbm14 morphants displayed reduced cell proliferation in MZT. 30 embryos in each condition were used to quantify cell number (D), cell size (E), and nuclear size (F). Each sample dot represents the average value from each embryo."
NCBI gene IDs:
"Rbm14: 323721///402858"