EMBO/SourceData huggingface dataset:
Figure legend: "(G) Quantification of shortest distance between centroid of DNA and cortical actomyosin  network. While chromatin is able to reach close to the cortex in both siControl and siRACGAP1  treated cells, the distance of chromatin  from cortex  increases upon  ZM treatment and is further enhanced in the double treatment condition. Data represented as mean±SD. siControl - 4.7±0.87, siRACGAP1 - 4.4±0.64,  ZM - 5.47±0.87, siRACGAP1+ZM - 6.35±1.14 microns. Ordinary one-way ANOVA with multiple comparison, siControl vs ZM, ***p=0.002, siControl vs siRACGAP1, p=0.2823, siControl vs siRACGAP1+ZM, ****p&lt;0.0001, siRACGAP1 vs siRACGAP1+ZM, ****p &lt;0.0001, ZM  vs siRACGAP1, ****p&lt;0.0001, ZM  vs siRACGAP1+ZM, ***p=0.0004 ."
NCBI gene IDs:
"RACGAP1: 29127"
Figure legend: "HeLa CCL2 cells stably expressing control vector only (+vector), β-catenin WT (+WTR), S60A mutant (+S60AR), or S60D mutant (+S60DR) were generated using lentiviral infection and then infected with β-catenin knockdown lentivirus (shβ-catenin). A dot density graph with a box plot shows the percentage (%) of cells with cytokinesis-defective phenotypes (C). The box plot represents: central blue band, Median; box, Interquartile range; upper whisker, Maximum observation below upper fence; lower whisker, Minimum observation above lower fence; dot, Outlier. Each symbol in the graph represents a percentage of more than 30 cells from three independent experiments. ***, p&lt;0.001 (one-way ANOVA); Scale bars, 10 μm."
NCBI gene IDs:
"β-catenin: 12387"
Figure legend: "HeLa CCL2 cells transiently transfected with either GFP-β-catenin WT or GFP-β-catenin S60A were monitored during cell cycle progression by live cell imaging (E). The subcellular localizations of GFP-tagged β-catenin WT or S60A mutant construct were confirmed by immunofluorescence with anti-GFP antibody. The cells were additionally stained for Plk1 (see Appendix Figure S5A). The specific localization of GFP-β-catenin WT at the midbody was observed under a confocal laser microscope with high magnification (E, left panel). Cytokinesis-defective cellular phenotypes in each transfected HeLa cell were monitored (E, right panel)"
NCBI gene IDs:
"GFP: 
β-catenin: 12387"
Figure legend: "(G, H) HeLa CCl2 cells infected with either sh-control luciferase (shGL) or shβ-catenin lentiviruses were co-immunostained with anti-β-catenin and anti-Ect2 antibodies. Localization of β-catenin and Ect2 in the midbody of telophase cells was observed using a confocal microscope (G) and the signal intensity of Ect2 was monitored in the midbody of each knockdown telophase cell (H). Arrows indicate total β-catenin and Ect2 located at the midbody in telophase HeLa CCL2 cells (G). A dot density graph with a box plot shows the signal intensity of midbody-localized Ect2 in telophase cells. The box plot represents: central blue band, Median; box, Interquartile range; upper whisker, Maximum observation below upper fence; lower whisker, Minimum observation above lower fence; dot, Outlier. Approximately 70 cells from three independent experiments were measured. ***, p&lt;0.001 (unpaired two-tailed t test); Scale bars, 10 μm. The value of the relative neck thickness is shown in the lower left corner of the merged images (G)."
NCBI gene IDs:
"luciferase: 
β-catenin: 12387"
Figure legend: "(G) HeLa CCL2 cells were transfected with either wild type Flag−β-catenin (+WT), Flag-β-catenin S60A mutant (+S60A), or Flag-β-catenin S60D mutant (+S60D), then treated with 200 ng/mL nocodazole for 18 h (metaphase) and released by incubation in fresh medium for 2 h (telophase). Cell lysates were used for pull-down analysis with rhotekin-RBD beads, and the pulled down beads were separated by 12 % SDS-PAGE followed by immunoblot analysis with the indicated antibodies. The membrane was then stained with Coomassie (CBB) to determine the amount of RBD precipitated."
NCBI gene IDs:
"Flag: 
β-catenin: 12387"
Figure legend: "(A) 786-0 or 769-P cells stably expressing shNT or shGDH1  were treated with or without aa starvation for the indicated  times, and then  cell viability was assessed. (B) 786-0  or 769-P  cells stably expressing shNT or shGDH1  were reconstituted with or without various amounts of rGDH1 WT (3*WT indicates 3-fold expression WT) and then treated with aa  starvation for the indicated  time. Cell viability  was subsequently assessed. ( Data information: The data are represented as means ± SEM of three independent experiments and all statistical analyses were conducted using unpaired t-test. (**p &lt; 0.01; ***p &lt; 0.001.)"
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(G) Immunofluorescence (IF) assay was performed to determine the subcellular location of WT or   mitochondrial  signal peptide deleted  (dMSP) GDH1. GDH1 localization was indicated by GDH1 antibody and MitoTracker  (left). Signal intensities and distances were quantified (right). Scale bars: 20 μm."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(H) Cytoplasm-mitochondrial fractionation assay  was performed to determine the subcellular location of WT or   mitochondrial signal peptide deleted (dMSP) GDH1. The fractionation efficiency  and GDH1 location were determined by WB using antibodies against  VDAC1 (mitochondrial membrane marker) and  GDH1. Tubulin was used as loading control."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(D) FLAG-tagged GDH1 (including WT and mutants with different  lysine mutations to  arginine) was co-transfected with  HA-tagged ubiquitin for 48 hours, and then cells were subjected to  aa  starvation for 12 hours before collecting cells.  WB and qPCR separately tested the levels of GDH1  protein and mRNA, respectively. Data information: The data are represented as the mean ± SEM of three independent experiments. The one-way ANOVA test (N.S.: not significant; *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001)."
NCBI gene IDs:
"FLAG: 
HA: 
GDH1: 2746
ubiquitin: 7323///7321///7322"
Figure legend: "(H) 786-0 or 769-P cells stably expressing shGDH1  were reconstituted with rGDH1 WT or 3KR mutant and then treated with or without aa starvation for the indicated time. Cell viability was determined. Unpaired two-tailed t-test was used. Data information: The data are represented as the mean ± SEM of three independent experiments. The unpaired t-test were used (N.S.: not significant; *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001)."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(H) 786-0  or 769-P cells (WT or stably expressing shGDH1) were transfected with siNC or siRNF213 for 48 hours and then were treated with or without aa starvation for the indicated times. Cell viability  was determined. Unpaired t-test was used with ***p &lt; 0.001."
NCBI gene IDs:
"GDH1: 2746
RNF213: 57674"
Figure legend: "786-0  cells expressing WT or enzymatically dead (ED) GDH1 were transfected with siNC or siRNF213 for 48 hours and then were treated with or without aa starvation for 12 hours. In the above cells in (A), qPCR was used to measure the mRNA of the indicated RP genes (C)."
NCBI gene IDs:
"GDH1: 2746
RNF213: 57674"
Figure legend: "Xenogeneic inoculation of 786-0  cells lacking  GDH1 with restored expression of WT, ED or 3KR mutant GDH1 into the left groin of nude mice. The solid tumor formed by the indicated 786-0  cells is shown (B)."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "Xenogeneic inoculation of 786-0  cells lacking GDH1 with restored expression of WT, ED or 3KR mutant GDH1 into the left groin of nude  mice. The staining of  Ki67 (G, upper panel) and TUNEL  (G, lower panel) using tumors from (B) are shown and analyzed (G, right panel). We used 6  mice in each group. Scale bars: 50 μm. Data information: All statistical analyses were conducted using unpaired t-test (n = 6). (N.S.: not significant; *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001)."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(A) WT, Irgm1-/-, Irgm2-/- and Irgm3-/- mice (n = 4 mice/genotype) were injected i.p. with LPS (8 mg/kg). Serum was collected 4 hours post injection (hpi) and concentration of various cytokines determined via a preconfigured Luminex multiplex panel. Relative concentration (fold change relative mean of WT) is shown for the indicated cytokines (absolute cytokine concentrations of same experiment are shown in Fig EV1)."
NCBI gene IDs:
"Irgm3: 16145
Irgm1: 15944
Irgm2: 54396"
Figure legend: "(B) WT (n = 9), Irgm1-/- (n = 7), Irgm2-/- (n = 9) and Casp1-/-Casp11-/- (n = 7) mice were injected i.p. with LPS (8 mg/kg). Serum was collected 4 hpi and concentration of IL-1β, IL-18, and TNFα was measured via ELISA."
NCBI gene IDs:
"Casp1: 12362
Casp11: 12363
Irgm1: 15944
Irgm2: 54396"
Figure legend: "(A) qPCR measurement of IL-1β, and TNF-α mRNA levels in WT and Irgm2-/- BMMs following 8-hour stimulation with LPS (1 µg/ml)."
NCBI gene IDs:
"IL-1β: 16176
Irgm2: 54396
TNF-α: 21926"
Figure legend: "(B) WT, Irgm2-/- and Casp1-/-Casp11-/- BMMs were treated for 24 hours with LPS at indicated doses and supernatant TNFα, IL-1β, and IL-18 was measured by ELISA."
NCBI gene IDs:
"Casp1: 12362
Casp11: 12363
Irgm2: 54396"
Figure legend: "(C) IFNγ-primed WT and Irgm2-/- BMMs were treated with LPS, Pam3CSK4, poly(I:C) or a combination of Pam3CSK4 and poly(I:C) (1 µg/ml for all treatments) for 24 hours and cell supernatant IL-1β and IL-18 concentrations were assessed by ELISA."
NCBI gene IDs:
"Irgm2: 54396"
Figure legend: "(D) WT, Irgm2-/-, Casp1-/-Casp11-/- and Irgm2-/-Casp1-/-Casp11-/- BMMs were treated with LPS (1 µg/ml) and IL-1β, IL-18, and LDH release were assessed at 24 hours post treatment (hpt)."
NCBI gene IDs:
"Casp1: 12362
Casp11: 12363
Irgm2: 54396"
Figure legend: "(E) IFNγ-primed WT, Irgm2-/-, Casp1-/-Casp11-/- and Irgm2-/-Casp1-/-Casp11-/- BMMs were treated with LPS (5 µg/ml) for 4 hours and subsequently stained with anti-ASC antibody and Hoechst stain (DNA/nuclei). Representative images are shown with white arrows pointing at ASC specks. Number of ASC specks per nuclei was quantified. Scale bars: 20 µm."
NCBI gene IDs:
"Casp1: 12362
Casp11: 12363
Irgm2: 54396"
Figure legend: "(F) IFNγ-primed WT, Irgm2-/-, Casp1-/-Casp11-/- and Irgm2-/-Casp1-/-Casp11-/- BMMs were treated with LPS (1 µg/ml) for 24 h and cell lysates and supernatants collected. Protein levels in cell lysates (caspase-1, and actin) and supernatants (caspase-1 p20) were visualized via immunoblotting."
NCBI gene IDs:
"Casp1: 12362
Casp11: 12363
Irgm2: 54396"
Figure legend: "(A) IFNγ primed WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were treated with LPS (5 µg/ml) for 4 h. Following treatment, cells were stained with anti-ASC antibody and Hoechst (DNA/nuclei). Representative images of ASC specks (white arrows point at specks) are shown and number of ASC specks per nuclei quantified. Scale bars: 20 µm."
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: "(B) WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were treated with LPS (1 µg/ml). IL-1β, IL-18, and LDH release were assessed at 24 hours-post treatment (hpt)."
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (C) WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were infected with E. coli K-12 at indicated MOIs and cell viability assessed via CellTiter-Glo. Cell death was calculated as a function of relative viability to uninfected cells. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (D) WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were infected with E. coli K-12 (MOI 25) and 24 hpi supernatant IL-1β and IL-18 levels were measured by ELISA. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (E) WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were treated with OMVs at indicated concentrations for 24 h. Cell viability was assessed via CellTiter-Glo and cell death was calculated as a function of relative viability to untreated cells. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (F) WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were treated with OMVs (1 µg/ml) and 24 hpt cell supernatant IL-1β and IL-18 levels were measured via ELISA. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (A) WT, and Irgm2-/- BMMs were stimulated overnight with IFNγ or left untreated and cell lysates were collected. Lysates were assessed for Casp11, Gbp2, and actin protein levels via immunoblotting. "
NCBI gene IDs:
"Irgm2: 54396"
Figure legend: " (B) IFNγ-primed and unprimed WT, Irgm2-/-, Gbpchr3-/- and Aim2-/- BMMs were infected with Francisella novicida (MOI 10) and LDH release measured at 4 hpi. "
NCBI gene IDs:
"Aim2: 383619
Gbp: 14468
Irgm2: 54396"
Figure legend: " (C) WT, Irgm2-/-, Gbpchr3-/- and Irgm2-/- Gbpchr3-/- BMMs were treated with LPS (1 µg/ml). IL-1β, IL-18, and LDH release were assessed at 24 hpt. "
NCBI gene IDs:
"Gbp: 14468
Irgm2: 54396"
Figure legend: " (D) IFNγ-primed WT, Irgm2-/-, Gbpchr3-/- and Irgm2-/- Gbpchr3-/- BMMs were treated with LPS (1 µg/ml) for 24 hours and cell lysates and supernatants collected. Protein levels in cell lysates (Caspase-1, and actin) and supernatants (Caspase-1 p20) were visualized via immunoblotting. "
NCBI gene IDs:
"Gbp: 14468
Irgm2: 54396"
Figure legend: " (A) WT, Irgm2-/-, and Nlrp3-/- BMMs were treated with LPS (0.1 µg/ml) for 3 h followed by nigericin for 1 h and IL-1β/LDH release was measured. "
NCBI gene IDs:
"Irgm2: 54396
Nlrp3: 216799"
Figure legend: " (B) WT, Irgm2-/-, Casp11-/- and Irgm2-/-Casp11-/- BMMs were treated with LPS (0.1 µg/ml) for 3 h followed by nigericin and/or MCC950 for 1 h and IL-1β/LDH release was measured. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (C) WT, Irgm2-/-, Casp11-/- and Irgm2-/-Casp11-/- BMMs were treated with LPS (1 µg/ml) and/or MCC950 for 24 h and IL-1β/LDH release was measured. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (D) IFNγ-primed WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were transfected with LPS using lipofectamine LTX and LDH release was measured at 2 hpt. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (E) IFNγ-primed WT, Irgm2-/-, Casp11-/- and Irgm2-/- Casp11-/- BMMs were co-treated with LPS (indicated doses) and Listeria monocytogenes (MOI 10) and LDH release measured at 4 hpt. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (A) WT (n = 9), Irgm2-/- (n = 10), Casp11-/- (n = 7) and Irgm2-/-Casp11-/- (n = 8) mice were injected i.p. with LPS (8 mg/kg). Morbidity and mortality were observed for 48 h at 3 hour intervals. "
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (B) WT (n = 9), Irgm2-/- (n = 8), Casp11-/- (n = 7) and Irgm2-/-Casp11-/- (n = 10) mice were injected i.p. with LPS (8 mg/kg). Serum was collected 4 hpi and concentration of IL-1β, and IL-18 was measured by ELISA. Data shown are from 2 pooled experiments."
NCBI gene IDs:
"Casp11: 12363
Irgm2: 54396"
Figure legend: " (A) WT, Irgm2-/-, Irgm3-/-, Irgm2-/-Irgm3-/-, panIrgm-/-, and Casp11-/- BMMs were treated with LPS (1 µg/ml) or infected with E. coli K-12 (MOI 25) and IL-1β, and LDH release were assessed at 24 hpt (n = 3 independent experiments). (B) IFNγ-primed WT, Irgm2-/-, Irgm3-/-, Irgm2-/-Irgm3-/-, panIrgm-/-, and Casp11-/- BMMs were treated with LPS (1 µg/ml) or infected with E. coli K-12 (MOI 25) and IL-1β, and LDH release were assessed at 8 hpt/ hpi (n = 3 independent experiments). "
NCBI gene IDs:
"Casp11: 12363
Irgm3: 16145
Irgm: 15944
Irgm2: 54396"
Figure legend: " (C) WT (n = 15), Irgm2-/- (n = 12), and panIrgm-/- (n = 13) mice were injected i.p. with LPS (8 mg/kg). Serum was collected 4 hpi and concentration of IL-1β and TNFα was measured via ELISA. Data shown are from 3 pooled experiments. "
NCBI gene IDs:
"Irgm: 15944
Irgm2: 54396"
Figure legend: " (D) WT, Irgm2-/-, panIrgm-/- and Casp11-/- mice (n = 8 mice/genotype) were injected i.p. with LPS (8 mg/kg body weight). Morbidity and mortality were observed for 48 h at 3-h intervals. "
NCBI gene IDs:
"Casp11: 12363
Irgm: 15944
Irgm2: 54396"
Figure legend: "A,B Tumor growth curve (left) and summary of end-point tumor masses (right) of 6-8 week-old wildtype (WT) and Peli1-KO (KO) mice inoculated s.c. with B16F10 (A; WT, n=7; KO, n=6) or E.G7 (B; WT, n=5; KO, n=5) tumor cells."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "C,D Flow cytometry analysis of CD4+ and CD8+ T cells in CD45.2+ TILs and dLN cells from B16F10 tumor-bearing wildtype and Peli1-KO mice, presented as a representative plot (C) and summary graph based on multiple mice (D; WT, n=6; KO, n=6)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "E,F Flow cytometry analysis of IFNγ-producing CD4+ and CD8+ T cells in TILs, and dLN cells of B16F10 tumor-bearing (day 19) wildtype and Peli1-KO mice, presented as a representative plot (E) and summary graph (F). (WT, n=6; KO, n=6)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "G,H Flow cytometry analysis of IFNγ+CD8+ T cells in TILs of E.G7 tumor-bearing (day 24) wildtype and Peli1-KO mice, presented as a representative plot (G) and summary graph (H). (WT, n=3; KO, n=3)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "A,B Tumor growth curve (left) and summary graph of end-point tumor masses (right) of wildtype (WT) and Peli1-TKO (TKO) mice inoculated s.c. with B16-OVA (A; WT, n=5; KO, n=5) or E.G7 (B; WT, n=6; KO, n=4) tumor cells."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "C Flow cytometry analysis of IFNγ-producing CD4+ and CD8+ T cells in TILs and dLN cells from B16OVA tumor-bearing wildtype and Peli1-TKO mice, presented as a representative plot (upper) and summary graph (lower). (WT, n=5; KO, n=5)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "D Flow cytometry analysis of GZMB+CD8+ T cells in TILs from B16-OVA tumor-bearing wildtype and Peli1-TKO mice, presented as a representative plot (upper) and summary graph (lower). (WT, n=5; KO, n=5)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "E Tumor growth curve (left) and summary graph of end-point tumor masses (right) of B16-OVA tumor-bearing wildtype and the indicated Peli1 conditional KO mice. (Treg KO model: WT, n=5; Treg KO, n=6; MKO model: WT, n=3; MKO, n=5; BKO model: WT, n=9; BKO, n=9)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "A,B Seahorse analysis of basal (glucose injection) and maximal (oligomycin injection) ECAR or basal (no treatment) and maximal (FCCP injection) OCR in WT and Peli1-KO OT-I CD8 T cells that were either untreated (naïve) or activated with plate-bound anti-CD3 (1 μg/ml) plus anti-CD28 (1 μg/ml) for 16 h. Data are presented as a representative plot (A) or summary graph based on 6 wildtype and 6 KO mice (B)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "qRT-PCR analysis of the indicated genes using RNAs isolated from wildtype or Peli1-KO OT-I CD8 T cells stimulated with anti-CD3 and anti-CD28 for 6 h."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "D qRT-PCR analysis of the indicated genes using RNAs isolated from wildtype or Peli1-KO OT-I CD8 T cells stimulated with anti-CD3 and anti-CD28 for 6 h."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "E Immunoblot analysis of HK2 and Glut1 in wildtype or Peli1-KO OT-I CD8 T cells that were stimulated with plate-bound anti-CD3 and anti-CD28 for 24 h."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "A Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of freshly isolated CD8 T cells from wildtype (WT) or Peli1 KO (KO) mice (6 weeks old). Data are presented as a representative blot (left) and summary graphs of densitometric quantifications of the indicated proteins (presented as phosphorylated/total protein ratio) based on three independent experiments."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "B,C Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype or Peli1-KO OT-I CD8 T cells that were first rested on ice for 15 min and then stimulated in low-serum (1% FBS) medium with anti-CD3 and anti-CD28 for the indicated time periods."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "D,E Seahorse analysis of basal and maximal ECAR (D) or OCR (E) using wildtype or Peli1-KO OT-I CD8 T cells that were activated for 16 h with plate-bound anti-CD3 (1 μg/ml) plus anti-CD28 (1 μg/ml) in the presence of solvent control (DMSO), rapamycin (Rapa, 10 nM), or Torin 1 (100 nM). Data are presented as a representative plot (left) and summary graphs (right)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "F,G Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype or Peli1-KO OT-I CD8 T cells that were either not treated (-) or stimulated (+) for 1 h (F) or 24 h (G) with anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) in the presence (+) or absence (-) of rapamycin (10 nM) and Torin 1 (100 nM)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "H qRT-PCR analysis of Ifng gene expression in naïve wildtype or Peli1-KO OT-I CD8 T cells that were either not treated (-) or stimulated for 6 h (+) with anti-CD3 plus anti-CD28 in the presence (+) or absence (-) or rapamycin."
NCBI gene IDs:
"Ifng: 15978
Peli1: 67245"
Figure legend: "I-K Schematic of experimental design (I), tumor growth curve (J), and summary of day 15 tumor weight (K) of B6.SJL mice inoculated with B16-OVA melanoma cells (2 x 105) and adoptively transferred on day 7 with in vitro activated and rapamycin- or DMSO-treated wildtype or Peli1-KO OT-I CD8 T cells (4 x 105)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype (WT) or Peli1-KO (KO) OT-I CD8 T cells that were activated for 16 h with plate-coated anti-CD3 (1 μg/ml) plus anti-CD28, starved for 6 h in serum-free RPMI medium, and then treated for indicated time periods with FBS (10%) (A)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype (WT) or Peli1-KO (KO) OT-I CD8 T cells that were activated for 16 h with plate-coated anti-CD3 (1 μg/ml) plus anti-CD28, starved for 6 h in serum-free RPMI medium, and then treated for indicated time periods with insulin (B)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype (WT) or Peli1-KO (KO) MEFs that were starved for 16 h in serum-free DMEM medium and then treated for the indicated time periods with FBS (C)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype (WT) or Peli1-KO (KO) MEFs that were starved for 16 h in serum-free DMEM medium and then treated for the indicated time periods with insulin (D)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of wildtype (WT) or Peli1-KO (KO) MEFs that were starved for 16 h in serum-free DMEM medium and then treated for the indicated time periods with EGF (E)."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "A Immunoblot analysis of the indicated proteins in whole-cell lysates of wildtype (WT) and Peli1-KO (KO) CD8 T cells that were either not treated (NT) or activated for 16 h with plate-bound anti-CD3 and anti-CD28. Data are presented as a representative blot (left panel) and a summary graph of quantified TSC1 (middle panel) and TSC2 (right panel) protein bands relative to the level of α-Tubulin."
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "Immunoblot analysis of the indicated proteins in whole-cell lysates of wildtype and Peli1-KO CD8 T cells that were activated for 1 h with anti-CD3 and anti-CD28 and then treated with cycloheximide (CHX) for the indicated time periods. Data are presented as a representative blot (B)"
NCBI gene IDs:
"Peli1: 67245"
Figure legend: "D Immunoblot analysis of TSC1 and TSC2 in wildtype or Peli1-KO CD8 T cells stimulated with anti-CD3 plus anti-CD28 for 1 h and then incubated with (+) or without (-) cycloheximide (CHX) and/or MG132 for 2 h. Data are presented as a representative blot (left panel) and a summary graph of quantified TSC1 (middle panel) and TSC2 (right panel) protein bands relative to the level of α-Tubulin."
NCBI gene IDs:
"Peli1: 67245"