EMBO/SourceData huggingface dataset:
Figure legend: "I. Representative images showing mTFP  (cyan) and Venus  (yellow) fluorescence in HBVPs  which have direct contact with control (upper panel) or MCT1  (lower panel) siRNA-treated BMECs. VECAD immunostaining  was used for endothelial staining. J. Quantification of the  mTFP/Venus  fluorescence ratio of laconic  in HBVPs  which have direct contact with control (siCON) or MCT1  (siMCT1) siRNA-treated BMECs. (n=8 from independent experiments. Error bars indicate the standard error of the mean (SEM) from unpaired Student's t test.)"
NCBI gene IDs:
"MCT1: 6566"
Figure legend: "K. Representative images showing mTFP (cyan) and Venus  (yellow) fluorescence in HBVPs  which have direct contact with control (upper panel) or MCT5  (lower panel) siRNA-treated BMECs. VECAD immunostaining  was used for endothelial staining. L. Quantification of the  mTFP/Venus  fluorescence ratio of laconic  in HBVPs  which have direct contact with control (siCON) or MCT5  (siMCT5) siRNA-treated BMECs. (n=8 from independent experiments. Error bars indicate the standard error of the mean (SEM) from unpaired Student's t test.)"
NCBI gene IDs:
"MCT5: 9122"
Figure legend: "D.  Western blot analysis showing expression   level of MCT12  and β-actin in HBVPs and BMECs. E. Western blot analysis for siRNA efficiency of MCT12."
NCBI gene IDs:
"MCT12: 387700"
Figure legend: "G. Representative images showing mTFP (cyan) and Venus  (yellow) fluorescence in control (upper panel) or MCT12  (lower panel) siRNA treated HBVPs  in a 2 well chamber assay system with BMECs. VECAD  (purple) immunostaining  was used for endothelial staining. H. Quantification of the mTFP/Venus  fluorescence ratio of Laconic  in control (blue) or MCT12 (orange) siRNA treated HBVPs. (n=8 from independent experiments. Error bars indicate the standard error of the mean (SEM) from unpaired Student's t test."
NCBI gene IDs:
"MCT12: 387700"
Figure legend: "A. Permeability  assay  using vibratome  section showing the cadaverine 555 dye (red), CD31  (green) and CD13  (purple) in the  brain vasculature of  Glut1iECKO mice  with (bottom) or without (top) lactate  administration. Note the extravascular accumulation of cadaverine dye in control Glut1iECKO mice, but not in lactate  supplemented Glut1iECKO mice."
NCBI gene IDs:
"Glut1: 20525"
Figure legend: "B. Quantification of the CD13-positive pericytes  coverage in the brain vasculature  of  Glut1iECKO mice  with (orange) or without (blue) lactate  administration. (n=16 from 5 independent experiments. Error bars indicate the standard error of the mean (SEM) from 1-way ANOVA.) C. Quantification of the number of cadaverine 555 positive  brain parenchyma  cells  in the brain vasculature  of  Glut1iECKO mice  with (orange) or without (blue) lactate  administration. (n=24 from 5 independent experiments. Error bars indicate the standard error of the mean (SEM) from 1-way ANOVA.) D. Quantification of CSF lactate  level in WT and Glut1iECKO mice  with/without lactate  supplementation. (n=3 from independent experiments. Error bars indicate the standard error of the mean (SEM) from 1-way ANOVA.)"
NCBI gene IDs:
"Glut1: 20525"
Figure legend: "A. Western blot detection of PEX13, ATG7, SMURF1, and FANCC in HeLa/GFP-LC3cells transfected with the indicated siRNA. Asterisk denotes non-specific band."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "B. Representative images of GFP-LC3 colocalization with mCherry-capsid at 10 h after Sindbis virus (strain AO30) infection of HeLa/GFP-LC3 cells treated with the indicated siRNA. siPEX13 oligo #2 is shown; similar results were observed with three other siPEX13oligos. Arrowheads denote representative colocalized GFP-LC3/mCherry-capsid puncta. Scale bars, 10 μm."
NCBI gene IDs:
"PEX13: 5194"
Figure legend: "D. Western blot detection of Pex13 in MEFs of indicated genotype. Asterisks denote non-specific bands."
NCBI gene IDs:
"Pex13: 72129"
Figure legend: "A. Representative images of Parkin-mediated clearance of mitochondrial outer membrane protein TOMM20 in HeLa/Parkin cells treated with indicated siRNA 16 h after treatment with 10 μM CCCP or DMSO vehicle control. siPEX13 oligo #2 is shown; similar results were observed with three other siPEX13 oligos. Scale bars, 20 μm.B. Quantification of TOMM20 clearance in the experiment shown in (A). Results represent mean ± SEM of triplicate samples (~100 cells analyzed per sample). Similar results were observed in more than three independent experiments. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001; one-way ANOVA with adjustment for multiple comparisons."
NCBI gene IDs:
"PEX13: 5194"
Figure legend: "C. Representative images of Parkin-mediated clearance of mitochondrial double-stranded DNA (mtDNA) in HeLa/HA-Parkin cells treated with indicated siRNA 8 h after treatment with 2.5 μM oligomycin and 250 nM antimycin A (OA) or DMSO vehicle control. siPEX13 oligo #1 is shown; similar results were observed with three other siPEX13 oligos. Nuclear DNA staining was masked using DAPI. Scale bars, 20 μm.D. Quantification of mtDNA clearance in the experiment shown in (C). Results represent box plots of ~300 cells analyzed per sample. Whiskers represent 5%-95% range, and each outlier is represented by a dot. Similar results were observed in three independent experiments. ****p&lt;0.0001; Kruskal-Wallis H-test."
NCBI gene IDs:
"Parkin: 5071
PEX13: 5194"
Figure legend: "A. Western blot detection of PEX13 in HeLa/Parkin cells transfected with indicated siRNA and siRNA-resistant PEX13 plasmid. To achieve similar PEX13 protein expression levels, 0.75 μg PEX13 I326T and PEX13 W313G plasmids were transfected compared to 0.25 μg WT PEX13. Total plasmid level was adjusted using empty vector. Asterisk denotes non-specific band."
NCBI gene IDs:
"Parkin: 5071
PEX13: 5194"
Figure legend: "B. Representative images of Parkin-mediated clearance of TOMM20 in HeLa/Parkin cells treated with PEX13 siRNA and transfected with indicated PEX13 siRNA-resistant plasmid and then treated with CCCP (10 μM, 16 h). Scale bars, 20 μm. See Fig EV3B for representative images of mitochondrial morphology (TOMM20 staining) in control cells treated with DMSO."
NCBI gene IDs:
"Parkin: 5071
PEX13: 5194"
Figure legend: "D. Western blot detection of PEX13 expression in wild-type (WT) and PEX13 W313G (W313G) mutant primary human fibroblasts."
NCBI gene IDs:
"PEX13: 5194"
Figure legend: "E. Representative images of Parkin-mediated clearance of TOMM20 in wild-type (WT) and PEX13 W313G (W313G) mutant primary human fibroblasts treated with Oligomycin (2.5 μM) + Antimycin A (250 nM) for 24 h. Scale bars, 20 μm. W313G cells varied in morphology; outlined cell in upper right panel shows a representative cell with larger size and abnormal mitochondrial morphology (see Fig EV3C for higher resolution imaging) and outlined cell in lower right panel shows a representative cell with accumulation of fragmented mitochondria that would be scored as positive in (F).F. Quantification of experiment shown in (E). Results represent mean ± SEM of 5 groups of 10 images of random fields of cells (&gt;350 cells analyzed per sample). Similar results were observed in two independent experiments. ***p&lt;0.0001; two-tailed unpaired t-test."
NCBI gene IDs:
"PEX13: 5194"
Figure legend: "A-C Representative images of PEX13 and PMP70 (A), PEX13 and TOMM20 (B), or PEX13-Flag and WIPI2 (C) colocalization in HeLa/Parkin cells transfected with PEX13 after 4 h DMSO or CCCP treatment. Scale bars, 20 μm (A-B) and 5 μm (C)."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "D Western blot detection of endogenous PEX13 in HeLa/Parkin cells treated with 10 μM CCCP for the indicated time."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "A. Quantitative real-time PCR detection of mRNA levels for PEX3, PEX13, PEX14, and PEX19 in HeLa cells transfected with the indicated siRNA. ***p&lt;0.001; two-tailed unpaired t-test."
NCBI gene IDs:
"PEX13: 5194
PEX14: 5195
PEX19: 5824
PEX3: 8504"
Figure legend: "B. Quantification of Parkin-mediated TOMM20 clearance in HeLa/Parkin cells transfected with the indicated siRNA 16 h after treatment with 10 µM CCCP. Results represent mean ± SEM of triplicate samples (~100 cells analyzed per sample). Similar results were observed in three independent experiments. **p&lt;0.01, ***p&lt;0.001; one-way ANOVA with adjustment for multiple comparisons."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "C. Quantification of mtDNA clearance in HeLa/HA-Parkin cells transfected with the indicated siRNA 8 h after treatment with 2.5 μM oligomycin and 250 nM antimycin A (OA). Results represent box plots of ~300 cells analyzed per sample. Whiskers represent 5%-95% range, and each outlier is represented by a dot. Similar results were observed in three independent experiments. ****p&lt;0.0001, NS= not significant; Kruskal-Wallis H-test."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "(c) RNA expression changes for TFs related to 8-mer groups bZIP, CTCF, HIC1, and EGR. Significant changes relative to control samples are shown with asterisks (Wilcoxon rank sums test, two-sided)."
NCBI gene IDs:
"CTCF: 13018
EGR: 13653///13655///13654///13656
HIC1: 15248"
Figure legend: "(c) Chromosome 15 genome tracks neighboring Arc, displaying ATAC-seq read counts per million (CPM); RNA-seq CPM; H3K27ac normalized signal upon KCl stimulation, H3K4me1(Malik et al, 2014); CTCF(Sams et al, 2016); and Cortical neurons Hi-C data(Bonev et al, 2017). Red bars in ATAC-seq tracks indicate gained DA-peaks in BDNF, and red bars in RNA-seq tracks indicate Arc differential expression in BDNF and KCl 1h. Green blocks in Hi-C tracks indicate anchor points for the calculation of contact scores, using shaman(Cohen et al, 2017). Line and Spearman's rho value indicate counts rank-based correlation between highlighted peaks across all samples (Appendix Figure S4c). TF module names indicate the presence of 8-mers in those peaks."
NCBI gene IDs:
"Arc: 11838"
Figure legend: "G) Western blot  analysis of siRNA knockdown of p53  expression in three biological replicates (n=3). Β-actin serves as an internal loading control."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(c) ATG14L-containing VPS34 complexes were immunopurified from WT, ULK1−/− 2KD (ULK def.) and FIP200−/− MEFs, and measured for lipid kinase activity as in a."
NCBI gene IDs:
"FIP200: 12421
ULK1: 22241"
Figure legend: "(d) VPS34 was immunopurified from WT, ULK-deficient and FIP200−/− MEFs. A kinase assay was performed as in a."
NCBI gene IDs:
"FIP200: 12421
ULK: 71742///29869///22241"
Figure legend: "(e) Immunoprecipitation of Beclin-1-containing VPS34 complexes from WT, ULK-deficient and FIP200−/− MEFs. A kinase assay was performed as in a."
NCBI gene IDs:
"FIP200: 12421
ULK: 71742///29869///22241"
Figure legend: "(j) VPS34 activity with or without ULK1 overexpression was assayed. A representative experiment of four repeats is shown."
NCBI gene IDs:
"ULK1: 22241"
Figure legend: "(a) HEK293 cells were transfected with ATG14L, VPS34 and Beclin-1. ATG14L-containing VPS34 complexes were immunopurified and subjected to an in vitro ULK1 kinase assay in the presence of γ-32P[ATP]. Bound ATG14L complexes and soluble ULK1 were separated and phosphorylation was detected by autoradiography (AR, left panels). Western blotting was performed (right panels). Results are representative of two unique experiments."
NCBI gene IDs:
"ATG14L: 22863
ULK1: 8408"
Figure legend: "(b) Full-length murine GST-Beclin-1 and various truncations (as labelled) were subjected to an in vitro HA-ULK1 kinase assay. GST-Beclin-1 6(S/T)A has serine/threonine residues 4, 7, 10, 14, 29 and 42 mutated to alanine. ULK1 inputs were determined by western blotting, Beclin-1 inputs by Coomassie (Coom) and target phosphorylation by autoradiography."
NCBI gene IDs:
"Beclin-1: 56208"
Figure legend: "(c) GST-Beclin-1 (1-85) was subjected to an in vitro ULK1 kinase reaction and analysed by mass spectrometry. Ser 14, Ser 15 in humans (outlined), is the main phosphorylation site identified (for mass spectrometry data, see Supplementary Fig. S2b,c). Mass spectrometry was performed on a single experiment."
NCBI gene IDs:
"Beclin-1: 56208"
Figure legend: "(d) Beclin-1 Ser 14 is the main in vitro ULK1 phosphorylation site. Beclin-1 WT, and S4A and S14A mutants were subjected to an in vitro ULK1 kinase assay. The reaction was developed by autoradiography and stained for Beclin-1 input levels by Coomassie blue. Results are representative of two unique experiments."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "(e) HEK293 cells were transfected with the indicated plasmids under nutrient-rich conditions. Beclin-1 was immunoprecipitated and immunoblotted with pBeclin-1(Ser 14), or anti-Beclin-1 as a loading control. ULK1 inputs are included below immunoprecipitated samples."
NCBI gene IDs:
"Beclin-1: 8678
ULK1: 8408"
Figure legend: "(f) Purified GST-Beclin-1 (1-85) was subjected to in vitro phosphorylation by GST-ULK1 (top panel) and GST-ULK2 (bottom panel). Reactions were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"ULK1: 8408
ULK2: 9706"
Figure legend: "(g) HEK293 cells were transfected with ATG14L, VPS34 and Beclin-1 and grown under nutrient-rich conditions. ATG14L-containing VPS34 complexes were immunoprecipitated and lipid kinase activity was assayed as described in Fig. 1j. Inputs were immunoblotted with the indicated antibodies. Representative of four unique experiments."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678
VPS34: 5289"
Figure legend: "(h) Stable lines containing Beclin-1(WT or S14A) were used for Beclin-1 immunoprecipitation. Binding partners were determined by SDS-PAGE analysis and western blot using the indicated antibodies. Uncropped images of blots are shown in Supplementary Fig. S4."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "(c) WT or FIP200−/− MEFs were incubated under nutrient-rich, amino-acid-deprived or Torin-1 (+T, an mTOR inhibitor) conditions. Beclin-1 was purified and immunoblotted as in Fig. 3b."
NCBI gene IDs:
"FIP200: 12421"
Figure legend: "(d) WT or ULK-deficient MEFs were incubated with or without amino acids. Beclin-1 was purified and immunoblotted as in a. Two unique experiments were performed. Uncropped images of blots are shown in Supplementary Fig. S4."
NCBI gene IDs:
"ULK: 71742///29869///22241"
Figure legend: "(a) Beclin-1 alone (lanes 1-4) or Beclin-1 and ATG14L (lanes 5-8) were overexpressed in HEK293 cells. Beclin-1 was purified either by direct immunoprecipitation (lanes 1-4) or by ATG14L immunoprecipitation (lanes 5-8). Immunoprecipitated samples were subjected to an in vitro ULK1 kinase assay with increasing amounts of ULK1. Reactions were immunoblotted with the indicated antibodies. Black line denotes discontinuous lanes from the same gel. Two unique experiments were performed."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678
ULK1: 8408"
Figure legend: "(c) An ATG14L-FLAG-6His-inducible U2OS cell line was induced for 16 h in the presence of amino acids. Endogenous Beclin-1 was immunoprecipitated and immunoblotted as in Fig. 3a. ATG14L input levels were detected by immunoblotting. Two unique experiments were performed."
NCBI gene IDs:
"ATG14L: 22863"
Figure legend: "(d) HEK293 cells transfected with either ATG14L or Beclin-1, or both, in conjunction with ULK1, were immunoprecipitated as indicated and blotted with the indicated antibodies."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678
ULK1: 8408"
Figure legend: "(e) HEK293 cells were transfected with Beclin-1 and ULK1 in the presence of ATG14L WT or ATG14LΔCCD, which is defective in Beclin-1 binding, under nutrient-rich conditions. Lysates were resolved by SDS-PAGE and blotted with the indicated antibodies."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678
ULK1: 8408"
Figure legend: "(f) HEK293 cells were transfected with ULK1 and Beclin-1 in conjunction with either ATG14L WT, or one of two mutants (ΔBATS,ΔN) that are defective in phagophore localization. Samples were handled as in e. Uncropped images of blots are shown in Supplementary Fig. S4. Lysates were resolved by SDS-PAGE and blotted with the indicated antibodies."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678
ULK1: 8408"
Figure legend: "(a) HEK293 cells were transfected with Beclin-1, with or without UVRAG, in conjunction with ULK1 as indicated in the presence of amino acids. Lysates were immunoblotted with the indicated antibodies. A representative experiment of three repeats is shown."
NCBI gene IDs:
"Beclin-1: 8678
ULK1: 8408
UVRAG: 7405"
Figure legend: "(b) UVRAG bridges the interaction between Beclin-1 and ULK1. HEK293 cells were transfected with Beclin-1, with or without UVRAG, in conjunction with ULK1 as indicated. Lysates were immunoprecipitated with anti-HA(Beclin-1) antibody and blotted with the indicated antibodies. A representative experiment of three repeats is shown. Uncropped images of blots are shown in Supplementary Fig. S4."
NCBI gene IDs:
"Beclin-1: 8678
ULK1: 8408
UVRAG: 7405"
Figure legend: "(b) HEK293 cells transfected with Beclin-1 ATG14L were grown in the presence or absence of amino acids. Lysates were immunoblotted with the indicated antibodies (left panel) and quantified by densitometry (right panel). Data represent mean+s.d. of three unique experiments."
NCBI gene IDs:
"ATG14L: 22863
Beclin-1: 8678"
Figure legend: "(c) Beclin-1-shRNA reconstituted lines (WT or mutant) and controls (scramble shRNA or Beclin-1 shRNA) were grown with or without amino acids and assessed for autophagy (left panel) and Beclin-1 levels (right panel)."
NCBI gene IDs:
"Beclin-1: 8678"
Figure legend: "(f) HA-Beclin-1 WT or S14D was transiently expressed in FIP200−/− MEFs grown under nutrient-rich conditions. Indirect immunofluorescence was performed using antibodies against endogenous LC3B and HA-Beclin-1. Scale bars, 20 μm. (g) Quantification of LC3B puncta from confocal in f. In the HA-Beclin-1- or HA-Beclin-1-S14D-transfected samples, only the HA-positive cells were counted for LC3B puncta. Error bars were processed as in e. Mean value shown; P values determined by Student's t-test using 10 unique fields of view from f. Uncropped images of blots are shown in Supplementary Fig. S4."
NCBI gene IDs:
"Beclin-1: 56208
FIP200: 12421"
Figure legend: "(a) Bec-1C. elegans were reconstituted with either WT or mutant GFP-BEC-1. Stable worm lines with Bec-1 rescue were obtained and embryos were stained with anti- PGL-1 antibody. Arrowheads indicate normal PGL-1 staining in germline cells. (b) Quantification of PGL-1 puncta outside germline cells (left panel). Error bars represents s.d. between 3 unique embryos in a representative experiment. Reconstituted Bec-1 (WT and mutant) levels in Bec−/− stable worms were compared by western blotting. Mean value presented."
NCBI gene IDs:
"Bec-1: 177345"
Figure legend: "(c) Spectrum of defects in PGL granule degradation in bec-1 mutant rescue embryos. Mutant embryos exhibited either high levels of diffuse PGL-1 staining (middle-left panel; one-third of the embryos), or large punctuate PGL-1 structures in somatic cells (bottom-left panel; two-thirds of the embryos). Both diffuse or punctuate PGL-1 staining in somatic cells have been described in autophagy-deficient embryos."
NCBI gene IDs:
"bec-1: 177345"
Figure legend: "(d) Embryos from the lines described in a were labelled with anti-LGG-1, along with WT and unc-51 worms. Representative embryos at ∼ 100 cell stage are shown. (e) Quantification of LGG-1 per embryo from labelling in d. Error bars generated as in b. Mean value presented. Uncropped images of blots are shown in Supplementary Fig. S4. Scale bar, 10 μm."
NCBI gene IDs:
"unc-51: 180311"
Figure legend: "(A) 293T cells were co-transfected with expression plasmids for Cherry-STING, the murine IFNβ-luciferase reporter (IFNβ-Luc), a Renilla luciferase normalization control (pRL-TK) and the indicated expression plasmids or empty vector (ev). Cells were additionally co-transfected with expression plasmids for cGAS-GFP (stimulated) or IRES-GFP (unstimulated). 20 hours post transfection, cells were lysed and a dual-luciferase assay was performed. Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"Cherry: 
GFP: 
Luc: 
luciferase: 
pRL: 
TK: 
cGAS: 115004
IFNβ: 15977
STING: 72512"
Figure legend: "(B) An expression plasmid for RIG-I N (stimulated) or ev (unstimulated) was co-transfected with IFNβ-Luc, pRL-TK and the indicated expression plasmids in 293T cells and analyzed as in (A). Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"Luc: 
pRL: 
RIG-I: 
TK: 
IFNβ: 15977"
Figure legend: "(C) An expression plasmid for TBK1 (stimulated) or ev (unstimulated) was co-transfected together with IFNβ-Luc, pRL-TK and the indicated expression plasmids and analyzed as in (A). Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"Luc: 
pRL: 
TK: 
IFNβ: 15977
TBK1: 56480"
Figure legend: "(D) 293T cells were co-transfected with a plasmid expressing constitutively active IRF3 (IRF3-5D; stimulated) or IRES-GFP (unstimulated) together with IFNβ-Luc, pRL-TK and the indicated expression plasmids and analyzed as in (A). Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"GFP: 
Luc: 
pRL: 
TK: 
IFNβ: 15977
IRF3: 3661"
Figure legend: "(E) The ISG56-luciferase reporter, pRL-TK and the indicated expression plasmids were co-transfected in 293T cells. 24 hours post transfection, cells were stimulated with 0.1 ng/µl human IFNβ or mock stimulated and analyzed 16 hours later as described in (A). Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"ISG56: 
luciferase: 
pRL: 
TK: "
Figure legend: "(F-G) iMEFgt/gt stably expressing Cherry-STING and either ev or V5-tagged m152 were stimulated with 5 µg/ml ISD (F), 10 µg/ml poly(I:C) (G) or mock stimulated with Lipofectamine. 4 hours post stimulation, RNA was extracted to determine IFNβ mRNA transcripts by qRT-PCR. Data information: (A-G) Data is combined from three independent experiments."
NCBI gene IDs:
"Cherry: 
m152: 
V5: 
IFNβ: 15977
STING: 72512"
Figure legend: "(H-K) iBMDM stably expressing ev or m152-V5 were stimulated in duplicates with 10 µg/ml cGAMP (H), 6 (H) or 16 (I-K) hours later, secreted IFNβ (H-J) levels were determined by ELISA. (H-K) Experiments were performed three (H, I, K) or two (J) times independently and one representative experiment is shown. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Data are shown as mean ± SD. "
NCBI gene IDs:
"m152: 
V5: "
Figure legend: "(H-K) iBMDM stably expressing ev or m152-V5 were stimulated in duplicates with 5 µg/ml ISD (I), 6 (H) or 16 (I-K) hours later, secreted IFNβ (H-J) levels were determined by ELISA. (H-K) Experiments were performed three (H, I, K) or two (J) times independently and one representative experiment is shown. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Data are shown as mean ± SD. "
NCBI gene IDs:
"m152: 
V5: "
Figure legend: "(H-K) iBMDM stably expressing ev or m152-V5 were stimulated in duplicates with Newcastle's Disease Virus (NDV) infection (J) 6 (H) or 16 (I-K) hours later, secreted IFNβ (H-J) levels were determined by ELISA. (H-K) Experiments were performed three (H, I, K) or two (J) times independently and one representative experiment is shown. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Data are shown as mean ± SD. "
NCBI gene IDs:
"m152: 
V5: "
Figure legend: "(H-K) iBMDM stably expressing ev or m152-V5 were stimulated in duplicates with µM CpG DNA (K). 6 (H) or 16 (I-K) hours later, TNFα (K) levels were determined by ELISA. (H-K) Experiments were performed three (H, I, K) or two (J) times independently and one representative experiment is shown. Student's t-test (unpaired, two-tailed), n.s. not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Data are shown as mean ± SD. "
NCBI gene IDs:
"CpG DNA: 
m152: 
V5: "
Figure legend: "(A) HeLa cells were co-transfected with expression plasmids for Cherry-STING, V5-tagged m152 and either ev (unstimulated) or cGAS-GFP (stimulated)."
NCBI gene IDs:
"Cherry: 
GFP: 
m152: 
V5: 
cGAS: 115004
STING: 72512"