EMBO/SourceData huggingface dataset:
Figure legend: "B Effect of LT treatment on NLRP1B protein level. 293T cells were transfected with Flag-NLRP1B expression plasmid for 24 h, and cells were then treated with WT LT (+) or its E687C mutant (-) for 4 h. Shown is the anti-Flag immunoblot of the total cell lysates."
NCBI gene IDs:
"Flag: 
NLRP1B: 637515"
Figure legend: "C RFP-ASC specks formation in 293T cells expressing an indicated NLRP1B variant. Cells were treated with LT for 3 h. Scale bar, 20 μm. 1-983 and 984-C (residues 984 to the C terminus) are the FIIND-mediated autocleaved fragments of NLRP1B."
NCBI gene IDs:
"NLRP1B: 637515"
Figure legend: "Effect of MG132 on LT-induced NLRP1B degradation. 293T cells expressing NLRP1B (1-983)-HA were treated with LT and MG132 for 8 h. Shown are the anti-HA and anti-tubulin immunoblots of the total cell lysates."
NCBI gene IDs:
"NLRP1B: 637515"
Figure legend: "E Effect of MG132 and Leu on LT-induced NLRP1B degradation. 293T cells expressing NLRP1B (1-983)-HA were treated with LT and MG132 for 8 h. 10 mM Leu was added to cells 30 min prior to LT treatment. Shown are the anti-HA and anti-tubulin immunoblots of the total cell lysates."
NCBI gene IDs:
"HA: 
NLRP1B: 637515"
Figure legend: "F LT-induced ubiquitination of NLRP1B. 293T cells expressing NLRP1B (1-983)-HA were treated with LT and/or MG132 for 6 h. Cells were then harvested for anti-HA immunoprecipitation and subjected to immunoblotting analyses using indicated antibodies."
NCBI gene IDs:
"HA: 
NLRP1B: 637515"
Figure legend: "(A) In situ proximity ligation assay (PLA) of ZEB1 with JUN, FOSL1 and TAZ shows close proximity of ZEB1 with JUN and FOSL1 but not TAZ in the nucleus of MDA-MB-231 cells indicated by red fluorescent dots. As negative control ZEB1 was transiently knocked down by siRNA. In the left panel representative microscopic images are shown. Scale bar = 20 µm. Quantification of the PLA is shown on the right."
NCBI gene IDs:
"ZEB1: 6935"
Figure legend: "(A) Definition of two different ZEB1 peak subsets based on the presence or absence of overlapping YAP and JUN peaks. The two illustrative genome browser images show one example of a ZEB1-only peak and one example of a ZEB1/YAP/JUN peak (at the EFEMP1 gene and within the SHROOM3 gene, respectively). Control (ctrl) is the input for ZEB1 and IgG for the YAP and JUN ChIP-seqs. ChIP-seqs and respective controls were scaled accordingly."
NCBI gene IDs:
"EFEMP1: 2202
SHROOM3: 57619"
Figure legend: "(A) qRT-PCR in TGFß treated MCF10A cells after siRNA mediated knockdown of ZEB1, FOSL1 or YAP compared to control knockdown cells. Knockdown efficiency of all three factors is shown on the left. The right panel shows their effects on 11 ZEB1/YAP/AP-1 activated target genes."
NCBI gene IDs:
"TGFß: 
FOSL1: 8061
AP-1: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(B) Overlap of ZEB1, YAP and JUN peaks at the promoter of CYR61, CTGF and ANKRD1 and in a known enhancer region of the DOCK9 gene."
NCBI gene IDs:
"ANKRD1: 27063
CYR61: 3491
CTGF: 1490
DOCK9: 23348"
Figure legend: "(C) ZEB1-only peak at the promoter of LLGL2, a well-known ZEB1 repressed target."
NCBI gene IDs:
"LLGL2: 3993"
Figure legend: "(D) Luciferase reporter assay in MDA-MB-231 cells comparing control and transient siRNA-mediated knockdown of ZEB1. Reporter constructs of regulatory regions of genes For reporter assays, firefly luciferase activity was normalized to co-transfected renilla luciferase."
NCBI gene IDs:
"luciferase: 
ZEB1: 6935"
Figure legend: "(E) Luciferase reporter assays with the ANKRD1 promoter and DOCK9 enhancer constructs in MCF7 cells upon overexpression of ZEB1, YAP and JUN/FOSL1 (AP-1) or different combinations of the factors. n = 5 (ANKRD1) and n = 3 (DOCK9); shown is mean ± s.e.m.; *P ≤ 0.05; ratio paired t-test. For reporter assays, firefly luciferase activity was normalized to co-transfected renilla luciferase."
NCBI gene IDs:
"luciferase: 
ANKRD1: 27063
DOCK9: 23348
FOSL1: 8061
AP-1: 3725
JUN: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(C) The upper panel shows a schematic representation of the ANKRD1 luciferase reporter constructs. The lower panel shows luciferase reporter assays with these different ANKRD1 promoter constructs in MCF7 cells upon overexpression of ZEB1, YAP and JUN/FOSL1 or different combinations of the factors. n = 4-5; shown is mean ± s.e.m.; *P ≤ 0.05; **P ≤ 0.01; ratio paired t-test. Firefly luciferase activity was normalized to co-transfected renilla luciferase."
NCBI gene IDs:
"luciferase: 
ANKRD1: 27063
FOSL1: 8061
JUN: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(D) Left panel: EMSA using nuclear extracts from MDA-MB-231 and labelled TEAD binding side as probe (black arrow). Competition of specific binding complex (read arrow) with cold probe (c), anti-JUN, -panTEAD, and -ZEB1 antisera. Right panel: recombinant GST-ZEB1, but not GST-Ctrl, only binds to Z-box."
NCBI gene IDs:
"GST: 
ZEB1: "
Figure legend: "(B) Comparison of ZEB1 ChIP-seq peaks with ZEB1-dependent changes in chromatin activation status identified by ATAC-seq on control and ZEB1 knock-down MDA-MB-231 cells. Percentage of ZEB1-only (n=5,963) and ZEB1/YAP/JUN peaks (n=1,993) falling into ZEB1 repressed or activated genomic regions are shown."
NCBI gene IDs:
"ZEB1: 6935"
Figure legend: "(C) Genome browser tracks of ZEB1, YAP and JUN ChIP-seq signal intensity in wild type cells overlaid to ATAC-seq signal intensity in wild type, control (shCtrl) and ZEB1 (shZEB1) knock-down MDA-MB-231 cells. One known ZEB1 repressed target (CDH1) and one activated target (ANKRD1) are shown."
NCBI gene IDs:
"ANKRD1: 27063
CDH1: 999
ZEB1: 6935"
Figure legend: "(A) Correlation of ZEB1 mRNA expression with the expression of YAP, FOSL1 and JUN across all breast cancer cell lines included in the cancer cell line encyclopedia (CCLE). rs: Spearman's rank correlation coefficient."
NCBI gene IDs:
"FOSL1: 8061
JUN: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(C) Heatmap of mRNA expression data of ZEB1, YAP, JUN and 9 selected activated ZEB1/YAP/AP-1 target genes in breast cancer patient samples (GSE18229). Genes were selected for known roles in tumour promoting properties like cell migration. Tumours in which ≥ 6 out of 9 genes of the selected ZEB1/YAP/AP-1 target gene set were expressed higher than the 60th percentile of expression were defined as &quot;tumour promoting gene set high&quot;; when expression of ≥ 6 out of the 9 genes was below the 40th percentile, tumours were defined as &quot;tumour promoting gene set low&quot;. Tumours were annotated according to their molecular subtype."
NCBI gene IDs:
"AP-1: 3725
JUN: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(E) Kaplan Meier plots from survival analyses of human breast cancers patient samples (GSE18229) showing relapse free survival (RFS) based on the combined expression of the 9 selected ZEB1/YAP/AP-1 activated targets HR: hazard ratio. p-value from log-rank test. (F) Kaplan Meier plots from survival analyses of human ER-/PR- breast cancers showing distant metastasis free survival (DMFS) based on the combined expression of the 9 selected ZEB1/YAP/AP-1 activated targets HR: hazard ratio. p-value from log-rank test."
NCBI gene IDs:
"AP-1: 3725
YAP: 10413
ZEB1: 6935"
Figure legend: "(A) TNF concentrations in supernatants of WT and Tnf-/- BMDMs unstimulated or stimulated with 10 ng/ml Pam3CSK4, quantified by ELISA. (Left) 0 h, 4 h and 8 h time points. (Right) Close-up of 0 h time point. Dashed line indicates mean of blank measurements. Data are represented as mean ± SD of 3 (4 h, 8 h) or 5 (0 h) biological replicates using cells from 2 (4 h, 8 h) or 3 (0 h) mice. Statistical significance was determined by two-tailed paired-sample t-test."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(E - J) Nuclear localization of mVenus-RelA upon stimulation with 10 ng/ml TNF (E - G) or 10 ng/ml Pam3CSK4 (H - J), measured over time in WT and Tnf-/- BMDMs by fluorescence microscopy and quantified by automated image analysis. (E, H) Each row of the heatmap represents the NFκB signaling trajectory of one cell. Trajectories are sorted by maximum amplitude. Example trajectories are shown below. Data from a single representative experiment is depicted. (F, I) Indicated dynamic features were calculated from signaling trajectories. Violin plots depict pooled data from 8 (F) or 6 (I) biological replicates (total # of cells: 5769/5238 (F), 3843/3899 (I)). Marker indicates median of distribution. Statistical significance was determined by Wilcoxon rank sum test. Number indicates log10(p-value). (G, J) Percentage of cells with NFκB trajectories categorized as oscillatory based on a frequency threshold among all responder cells in each experiment. Data are represented as mean ± SD of 8 (G) or 6 (J) biological replicates. Statistical significance was determined by two-tailed paired-sample t-test. (F, G) Some replicates for Tnf-/- BMDMs overlap with data"
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(B) IκBα mRNA expression in WT and Tnf-/- BMDMs stimulated with 10 ng/ml TNF over 2 h, determined by RT-qPCR. IκBα expression was normalized to GAPDH expression and then to maximum WT value in each time course replicate. Data are represented as mean ± SD of 5 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point. Some replicates for Tnf-/- BMDMs overlap with data"
NCBI gene IDs:
"GAPDH: 14433
IκBα: 18035
Tnf: 21926"
Figure legend: "Phosphorylation of IKK, JNK, ERK, and p38 and total IKK levels in WT and Tnf-/- BMDMs stimulated with 10 ng/ml TNF over 2 h, determined by SDS-PAGE followed by Western Blotting. (C) One of three representative experiments is shown. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "Phosphorylation of IKK levels in WT and Tnf-/- BMDMs stimulated with 10 ng/ml TNF over 2 h For quantification of p-IKK (D) levels, basal levels (0 min) were deducted per genotype before normalizing to maximum WT value in each experiment. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "Phosphorylation of JNK levels in WT and Tnf-/- BMDMs stimulated with 10 ng/ml TNF over 2 h, p-JNK (E) levels, basal levels (0 min) were deducted per genotype before normalizing to maximum WT value in each experiment. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(E) TNF receptor 1 (TNFR1, left), TNF receptor 2 (TNFR2, center), and CD11b (right) cell surface expression in Tnf-/- compared to WT BMDMs unstimulated or stimulated for 30 min with 10 ng/ml TNF, measured by flow cytometry. Per experiment, median fluorescence intensity average of 3 culture replicates in Tnf-/- cells is expressed as percent of expression in WT cells. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed one-sample t-test using a hypothesis mean of 100."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(F) (Left) TNF receptor 1 (TNFR1) cell surface expression at baseline in BMDMs pre-treated with TNFR1 siRNA, control (ctrl) siRNA, or untreated (-). Median fluorescence intensity is expressed as percent of expression in untreated WT cells. (Right) Percentage of cells with oscillatory NFκB activity (measured by fluorescence microscopy) among all responder cells upon indicated siRNA pre-treatment and stimulation with 10 ng/ml TNF. Per condition, 1032 - 1484 responding cells were analyzed. (Left, right) Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by one-tailed paired-sample t-test."
NCBI gene IDs:
"TNFR1: 21938"
Figure legend: "(D) IκBα mRNA expression in Tnf-/- and Tnf-/-p100-/- BMDMs stimulated with 10 ng/ml TNF over 2 h, determined by RT-qPCR. IκBα expression was normalized to GAPDH expression and then to maximum WT value also obtained with each replicate. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point. Data for Tnf-/- BMDMs is also included"
NCBI gene IDs:
"GAPDH: 14433
p100: 18034
IκBα: 18035
Tnf: 21926"
Figure legend: "(E) Levels of nuclear NFκB DNA binding activity in Tnf-/- and Tnf-/-p100-/- BMDMs stimulated with 10 ng/ml TNF over 2 h, determined by EMSA. Quantification of the NFκB-DNA complex was normalized to the quantified NFY-DNA complex, and then to maximum WT value also obtained with each replicate. Data are represented as mean ± SD of 3 biological replicates. Statistical significance was determined by two-tailed paired-sample t-test for each time point. Data for Tnf-/- BMDMs is also shown"
NCBI gene IDs:
"p100: 18034
Tnf: 21926"
Figure legend: "(F - H) Nuclear localization of mVenus-RelA upon stimulation with 10 ng/ml TNF was measured over time by fluorescence microscopy in Tnf-/- and Tnf-/-p100-/- BMDMs and quantified by automated image analysis. (F) Each row of the heatmap represents the NFκB signaling trajectory of one cell. Trajectories are sorted by maximum amplitude. Example trajectories are shown below. Data from a single representative experiment are depicted. (G) Indicated dynamic features were calculated from signaling trajectories. Violin plots depict pooled data from 6 biological replicates (total # of cells: 4030/4133). Marker indicates median of distribution. Statistical significance was determined by Wilcoxon rank sum test. Number indicates log10(p-value). (H) Percentage of cells with NFκB trajectories categorized as oscillatory based on a frequency threshold among all responder cells in each experiment. Data are represented as mean ± SD of 6 biological replicates. Statistical significance was determined by one-tailed paired-sample t-test. (G, H) Data for Tnf-/- BMDMs is also included"
NCBI gene IDs:
"p100: 18034
Tnf: 21926"
Figure legend: "(E) Volcano plot of single-cell gene expression in Tnf-/- vs. WT BMDMs upon TNF stimulation. Dashed lines indicate p-value threshold of 0.01 and absolute log2(FC) threshold of 0.5. Only genes induced by P3C4 or TNF stimulation over mock in WT cells are included (p-value threshold of 0.05 and log2(FC) threshold of 0.25)."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(F) Violin plots of single-cell gene expression in WT and Tnf-/- BMDMs upon mock, TNF, or Pam3CSK4 stimulation. The 10 genes shown are significantly upregulated in Tnf-/- vs. WT cells upon TNF stimulation (see (E))."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(D) Violin plot of log­2(FC) of normalized H3K4me1 ChIP-seq signal in TNF- or Pam3CSK4-stimulated over unstimulated WT or Tnf-/- BMDMs within Cluster 1 regions (533 regions). Statistical significance was determined by Kolmogorov-Smirnov test. Data are represented as mean of two biological replicates for each genomic region. In the boxplot, central band indicates the median, boxes the interquartile range (IQR), whiskers values within 1.5 IQR from the median."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(E) Examples of genomic regions with differential H3K4me1 signal in TNF stimulation in Cluster 1. Tracks for WT and Tnf-/- BMDMs unstimulated or stimulated with TNF or Pam3CSK4 in two biological replicates (R1, R2) are shown."
NCBI gene IDs:
"Tnf: 21926"
Figure legend: "(A-B) Intraphagosomal proteolysis and acidification assays show that loss of RNF115 increases phagosome maturation in BMA cells. (C) Complementing the RNF115 KO with WT RNF115 or a W259A mutant which is unable to bind to E2 enzymes, shows that RNF115 ligase activity is required for the increase of phagosome maturation."
NCBI gene IDs:
"RNF115: 67845"
Figure legend: "(C) Immunofluorescence micrographs of VAMP8 (green) and Syntaxin-7 (STX7; red) show strong colocation around 3 µm silica bead phagosomes (white) in BMA macrophages. Nuclei are stained with Dapi. Scale bar is 5 µm. To account for people with red-green colour-blindness, (D) Colocation of VAMP8 (green) and STX7 (red) on individual phagosomes between WT and RNF115 KO cells represented by intersection, Manders' M1 and overlap coefficients. Data is represented as a box and whisker plot of total values across biological duplicate experiments, where the whiskers represent the minimum to maximum values and the central band indicates the median."
NCBI gene IDs:
"RNF115: 67845"
Figure legend: "(D) Box and whisker plot represents the quantification of the percentage (%) of the hepatic lesion area in the livers from six wild-type (WT) and six RNF115 KO (KO) mice after 48 h infection with S. aureus. Haematoxylin and eosin (H&amp;E)-stained liver sections of one representative animal per genotype are shown on the right, dotted line depicts the damaged area."
NCBI gene IDs:
"RNF115: 67845"
Figure legend: "(E-F) Box and whisker plots represent the average cell counts per high power field (HPF) of CD3 positive T-cells (E), and CD68 positive macrophages (F) in the livers from six wild-type (WT) and six RNF115 KO (KO) mice after 48 h infection with S. aureus. Representative immunohistochemistry sections are shown on the right."
NCBI gene IDs:
"RNF115: 67845"
Figure legend: "A, B Representative transmission electron microscopy (TEM) images showing keratinocyte mitochondria (red arrow) in patient 1 (A) and in the Mbtps1-conditional knockout (cKO) mouse model (B). Scale bars: 2 µm."
NCBI gene IDs:
"Mbtps1: 56453"
Figure legend: "C, D Quantification of the mitochondrial number and morphology in patient 1 (C) and the Mbtps1-cKO mouse model (D). (C) Left panel: n = 13 biological replicates of normal individuals, n = 10 biological replicates of patient 1. Middle and right panel: n = 121 biological samples of normal individuals and patient 1. (D) Left panel: n = 23 biological replicates of Mbtps1-loxP mice; n = 15 biological replicates of Mbtps1-cKO mice. Middle panel and right panel: n =113 biological replicates of Mbtps1-loxP mice and Mbtps1-cKO."
NCBI gene IDs:
"Mbtps1: 56453"
Figure legend: "E Immunofluorescence experiments showed that mutant S1P (p.Val355Gly and p.Ter1053Arg) was diffusely localized in the cytosol and showed lower mitochondrial localization compared with wild-type S1P, which suggested that these variants disrupt its mitochondrial import. The white arrowheads indicate the colocation (yellow) of S1P (green) and the mitochondrial tracer (red). Scale bars: 20 μm (panels 1-4); scale bars: 2 μm (panel 5)."
NCBI gene IDs:
"S1P: 8720"
Figure legend: "G A component separation assay revealed a lower expression of mutant S1P (p.Val355Gly and p.Ter1053Arg) than wild-type S1P in mitochondria."
NCBI gene IDs:
"S1P: 8720"
Figure legend: "H The impaired binding of mutant S1P (p.Val355Gly and p.Ter1053Arg) to translocase of the outer membrane (TOM) 70 and translocase of the inner membrane (TIM) 23 was detected by a coimmunoprecipitation (Co-IP) assay."
NCBI gene IDs:
"S1P: 8720"
Figure legend: "B Unchanged mRNA levels and decreased protein levels of ETFA and ETFB were observed in the skin biopsy of patient 1. Quantitative RT-PCR analysis (upper panel, n = 6 biological replicates of normal controls; n = 6 technical replicates of patient 1) and immunohistochemistry (lower panel, n = 6 biological replicates; n = 3 technical replicates) of the S1P, ETFA and ETFB levels."
NCBI gene IDs:
"ETFA: 2108
ETFB: 2109"
Figure legend: "C Representative immunohistochemistry images of S1P, ETFA and ETFB in both Mbtps1-conditional knockout (cKO) and Mbtps1-loxP mice. Scale bars: 100 μm."
NCBI gene IDs:
"Mbtps1: 56453"
Figure legend: "D MBTPS1 gene knockout led to decreases in the ETFA and ETFB protein levels in vivo. Quantitative RT-PCR analysis (upper panel, n = 6 biological replicates) and immunohistochemistry (lower panel, n = 6 biological replicates) of the S1P, ETFA and ETFB levels in Mbtps1-cKO mice and Mbtps1-loxP mice."
NCBI gene IDs:
"ETFA: 110842
ETFB: 110826
Mbtps1: 56453
MBTPS1: 56453"
Figure legend: "E Cycloheximide (CHX) chase analysis showed that MBTPS1 knockout induced rapid degradation of ETFA and ETFB proteins in HaCaT cells. Left panel: Representative western blotting images of the ETFA and ETFB protein levels during CHX chase. Right panel: Quantification of the immunoblotting results corresponding to the left panel (n = 3 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "F Mutant S1P (p.Val355Gly and p.Ter1053Arg) only weakly interacted with ETFA and ETFB. We constructed Flag-tagged wild-type and mutant S1P HaCaT cell lines and then performed a Co-IP assay with Flag antibody, and the interaction between S1P and ETFA/ETFB was detected by immunoblotting."
NCBI gene IDs:
"S1P: 8720"
Figure legend: "G Wild-type S1P, but not mutant S1P (p.Val355Gly and p.Ter1053Arg), decreased the association between ETFA and ETFB. We performed a Co-IP assay with an ETFA antibody, and the interaction between ETFA and ETFB was detected by immunoblotting."
NCBI gene IDs:
"S1P: 8720"
Figure legend: "Mitochondrial respiration (OCR) in control (Ctrl) and MBTPS1-knockout (KO) HaCaT cells was quantified in real time using a Seahorse extracellular flux analyser. Right subpanels: Quantiﬁcation of basal respiration, maximal respiration, and OCR-coupled ATP production in mitochondrial respiration (n = 5 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "B Quantiﬁcation of relevant metabolites in mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis in Ctrl and MBTPS1-KO HaCaT cells (n = 4 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "C Nonmitochondrial respiration (ECAR) was quantified in real time using a Seahorse extracellular flux analyser. Quantification of non-glycolytic acidification, glycolysis, glycolytic capacity, and glycolytic reserve in Ctrl and MBTPS1-KO HaCaT cells (n = 4 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "D MBTPS1 knockout led to a decrease in global ATP production in HaCaT cells. Control and MBTPS1-KO HaCaT cells were cultured in 6-well dishes. A standard curve was generated to calculate the sample ATP concentrations using an ATP Lite Luminescence Assay Kit (n = 4 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "E MBTPS1 knockout significantly increased the generation of mitochondrial reactive oxygen species (Mito SOX) in HaCaT cells. Left panel: Representative immunofluorescence images of Mito SOX in HaCaT cells. Scale bars: 100 µm. Right panel: The immunofluorescence intensity was quantified to calculate the relative Mito ROS level in HaCaT cells (n = 4 biological replicates)."
NCBI gene IDs:
"MBTPS1: 8720"
Figure legend: "A The S1P deficiency-induced decrease in ETFA and ETFB was significantly reversed by riboflavin (Rib) in a concentration-dependent manner in MBTPS1-KO HaCaT cells. GAPDH was used as a loading control. MBTPS1-KO cells were initially cultured in DMEM for 24 h and supplemented with 0, 2.5, 5, or 10 µM riboflavin for 3 days. The expression of ETFA and ETFB was then detected by immunoblotting."
NCBI gene IDs:
"MBTPS1: 8720
S1P: 8720"
Figure legend: "B The S1P deficiency-induced abnormalities in mitochondrial respiration can be significantly reversed by riboflavin supplementation and ETFA/B overexpression in HaCaT cells. Left panel: Mitochondrial respiration (OCR) was quantified in real time using a Seahorse extracellular flux analyser."
NCBI gene IDs:
"ETFA: 2108
S1P: 8720"
Figure legend: "C The decreased global ATP production caused by S1P deficiency could be significantly reversed by riboflavin supplementation and ETFA/B overexpression in HaCaT cells (n = 4 biological replicates)."
NCBI gene IDs:
"ETFA: 2108
S1P: 8720"
Figure legend: "(A) H1299 cells were transfected with Myc-β-catenin, CK1α and MDMX mutants. Phosphorylation of β-catenin S45 was determined by western blot. Co-expression of MDMX blocked the increase of pS45 level. MDMXSG (W200S/W201G) and MDMX-1-300 were defective for CK1α binding."
NCBI gene IDs:
"Myc: 
CK1α: 1452
β-catenin: 1499
MDMX: 4194"
Figure legend: "(A, B) H1299 cells and human foreskin fibroblasts HFF were stably infected with lentivirus expressing MDMX and analyzed by western blot."
NCBI gene IDs:
"MDMX: 4194"