EMBO/SourceData huggingface dataset:
Figure legend: "A, B Detection of γb palmitoylation through the  biotin-switch assay in  N. benthamiana. The γb-3xFlag and γb3CS-3xFlag proteins were treated with (Hyd+) or without (Hyd-) hydroxylamine, the thioester  cleavage reagent. Lanes labelled ' Palmitoylation ' show γb-3xFlag or  γb3CS-3xFlag  amounts recovered from the neutravidin  beads. The loading controls indicate sample loading onto the neutravidin  beads. Data information: In (A, representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"γb: 962674"
Figure legend: "A Effects of γb palmitoylation on BSMV  replication. Movement-deficient BSMV  (RNAα  + RNAγ/RNAγ3CS) was agroinfiltrated into N. benthamiana leaves. At 3 dpi, total protein and RNA were extracted for Western and Northern blot analyses. Antibodies and probes used for molecular analyses are indicated on the right of each panel. Actin  was used to monitor equal protein loading, and methylene blue-stained rRNAs  served as RNA loading controls. Data information: representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"rRNAs: 
RNAα: 962673
RNAγ: 962674"
Figure legend: "B  Western blot  analyses of chloroplast localization  of γb  and  γb3CS. Transiently expressed αa-GFP  in intact chloroplasts  isolated from BSMVγb-3xFlag- or  BSMV3CS-3xFlag-infected N. benthamiana leaves at 4 dpi. Detection of γb-3xFlag and γb3CS-3xFlag with an anti-Flag  antibody; TGB1  analysis with an anti-TGB1  antibody; αa-GFP  detection with an anti-GFP  antibody. NTRC  was a loading control for intact chloroplasts Phosphoenolpyruvate carboxylase (PEPC) was used to assess cytosolic  contamination of isolated chloroplasts. Data information: , representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"Flag: 
GFP: 
αa: 962673
γb: 962674"
Figure legend: "C Subcellular localization   of γb-GFP  and γb3CS-GFP  in N. benthamiana epidermal leaf  cells. The first and second rows show  subcellular localization  of γb-GFP  and γb3CS-GFP  transient expression in leaves. The third and fourth rows show localization  of γb-GFP  and γb3CS-GFP  in BSMVγb-GFP- and BSMV3CS-GFP-infected leaves  at 3 dpi. Figures on the right indicate the normalized fluorescence intensities of the GFP  (green) or the chloroplast  autofluorescence (red) channels along the dashed white lines shown in the merged images of the left panel. Scale bars, 20 μm. Data information: representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"GFP: 
γb: 962674"
Figure legend: "D Subcellular localization  of γb-GFP  or γb3CS-GFP  in isolated N. benthamiana protoplasts   harvested at 3 dpi. The fifth and sixth columns show γb localization  in BSMVγb-GFP- (movement-deficient) N. benthamiana protoplasts  after treatment with 200 μM 2-BP inhibitor. The released protoplasts  were collected after 48 h incubation in DMSO  or 2-BP followed by confocal microscopy visualization. Figures at the bottom indicate the normalized fluorescence intensities of the GFP  (green) and the chloroplast   autofluorescence (red) channels along the dashed white lines in the merged images above. Scale bars, 20 μm. Data information: representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"GFP: 
γb: 962674"
Figure legend: "A Effects of γb palmitoylation on  virus cell-to-cell movement    at 3 dpi with the BSMV  duplex fluorescence (dfBSMV) reporter system The dfBSMVmγb mutant control contains an ATG substitution of the γb  initiation codon Red fluorescence produced from the mCherry  expression cassette illustrates the primary infection foci, whereas GFP  fluorescence outside the red region shows invaded cells surrounding the primary infection foci. Fluorescence was evaluated by confocal microscopy. Scale bars, 100 μm. B Quantification of virus movement  in Panel A. To determine the movement capacity of the viruses depicted in each panel, the mCherry  and GFP  fluorescent intensities were first determined by ImageJ software. Then, the intensities within the secondarily invaded cells were divided by the fluorescent mCherry  plus the GFP  intensities in the primary infection foci (n = 10). The Y-axis indicates the relative sizes of the green areas in comparison to that of the red-colored areas. Data information: In (A, representative data are shown and three biological replicates had similar results. In (B), the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles, whiskers indicate minimum and maximum values, central band indicates the median. Letters in the chart denote statistically significant differences among groups according to the Duncan's multiple range test (p &lt; 0.05)."
NCBI gene IDs:
"γb: 962674"
Figure legend: "D Biotin-switch assay  to access palmitoylation of γb  in BSMV-infected N. benthamiana leaves  at different timepoints. BSMVγb-3xFlag was treated with the hydroxylamine  (Hyd+) thioester  cleavage reagent. Equal sample amounts used for palmitoylation analyses were monitored by Western blot  analysis with an anti-Flag  antibody. Data information: In representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"Flag: 
γb: 962674"
Figure legend: "E Subcellular localization  of BSMVγb-GFP and BSMV3CS-GFP in  N. benthamiana leaf epidermal cells. The mCherry-HDEL    and mTalin-RFP  reporters were used as markers to indicate the  ER and actin localization  Figures at the bottom indicate the normalized fluorescence intensities of the GFP  and mCherry  channels along the white dashed lines in the merged images above. Scale bars, 10 μm. Data information: In E), representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"GFP: 
γb: 962674"
Figure legend: "A BiFC co-localization analyses of αa  and TGB1  in N. benthamiana leaf  cells  at 3 dpi.  YFPn  and YFPc was fused to the TGB1  and the αa  proteins, respectively. Combinations of γb, γb3CS and the TBSV P19  control are shown above the micrographs. YFP  signals were visualized by confocal microscopy at 3 dpi and depicted as a false-green color. Scale bars, 30 μm. B Quantification of the numbers of YFP  foci per visual field (n = 10). Data information: In (A, , representative data are shown and three biological replicates had similar results. In (B, the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles, whiskers indicate minimum and maximum values, central band indicates the median. Letters in the chart denote statistically significant differences among groups according to the Duncan's multiple range test (p &lt; 0.05)."
NCBI gene IDs:
"γb: 962674
P19: 1493957"
Figure legend: "C Co-IP assays to determine in vivo interactions  between TGB1 and γb  or γb3CS. N. benthamiana leaf  tissues co-agroinfiltrated with RNAα, RNAγγb-GFP  or RNAγ3CS-GFP, and TGB1-3xFlag plasmids were harvested at 3 dpi. Total proteins were immunoprecipitated  with anti-Flag  beads. Input and immunoprecipitated  proteins (IP) were analyzed by Western blotting  with anti-GFP  or anti-Flag  antibodies."
NCBI gene IDs:
"Flag: 
GFP: 
RNAα: 962673
TGB1: 962677
RNAγ: 962674
γb: 962674"
Figure legend: "D Confocal microscopy  analyses showing RFP-TGB1 localization  at the  chloroplast periphery  in N. benthamiana leaves  agroinfiltrated with αa-GFP  and CFP-γb plasmids. CFP-γb  or CFP-γb3CS were co-expressed with RFP-TGB1  and αa-GFP  in  N. benthamiana leaves. Chloroplast  autofluorescence was displayed in false pink color. The third and fourth rows show leaves treated with 400 μM 2-BP inhibitor or the DMSO  control at 1 dpi. Two days later, confocal microscopy  observations of RFP-TGB1, αa-GFP, and CFP-γb  were performed. Figures on the right indicate the normalized fluorescence intensity of GFP, RFP, and CFP  channels along the white dashed line in the merged confocal images. Scale bars, 10 μm. Data information: In , representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"CFP: 
GFP: 
RFP: 
αa: 962673
TGB1: 962677
γb: 962674"
Figure legend: "G Chloroplast  extraction experiment for detection of TGB1 chloroplast localization. Plasmids harboring αa-GFP  and RFP-TGB1  were co-infiltrated into  N. benthamiana along with CFP-γb  or CFP-γb3CS. Intact chloroplast  preparations isolated from infiltrated  N. benthamiana leaves  at 4 dpi were subjected to Western blot  analyses. RFP-TGB1  was analyzed with an anti-TGB1  antibody. The αa-GFP, CFP-γb, and CFP-γb3CS proteins were detected with an anti-GFP  antibody. NTRC  was the loading control for the chloroplast  preparations Phosphoenolpyruvate carboxylase (PEPC) served as a control to assess cytosolic  contamination of isolated chloroplasts. Data information: In G), representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"CFP: 
GFP: 
RFP: 
αa: 962673
TGB1: 962677
γb: 962674"
Figure legend: "B Analysis of γb palmitoylation in  NbPAT15- or NbPAT21-silenced N. benthamiana  plants. NbPAT15 and NbPAT21 were silenced by intron-spliced hairpin RNA-mediated RNAi approaches The γb-3xFlag   plasmid was agroinfiltrated into the leaves, followed by  biotin-switch assays  at 3 dpi. Data information: In (B representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"Flag: 
γb: 962674
PAT15: 109211026
PAT21: 109240893"
Figure legend: "C Effects of γb palmitoylation by NbPAT15  or NbPAT21  on BSMV cell-to-cell movement. Infiltrated leaves  were observed by confocal microscopy at 3 dpi. Red fluorescence produced from the mCherry  expression cassette shows the primary infection foci, and GFP  fluorescence outside the red regions shows virus spread into secondary infected cells. Scale bars, 100 μm. Data information: In C), representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"PAT15: 109211026
PAT21: 109240893"
Figure legend: "A, B BiFC assays  to identify interactions  of γb  with NbPAT15  and NbPAT21 during BSMV  infection in  N. benthamiana at 3 dpi. PAT15-YFPn and PAT21-YFPn were co-expressed with BSMVγb-YFPc. Chloroplast  autofluorescence is depicted as a false red color and mCherry-HDEL   provides the ER  marker. Figures on the right show the normalized fluorescence intensities of GFP, mCherry  and the chloroplast  autofluorescence channels along the white dashed lines in the merged images. Scale bars, 20 μm. Data information: In (A, B, representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"YFP: 
γb: 962674
PAT15: 109211026
PAT21: 109240893"
Figure legend: "C Co-localization  analyses of αa-GFP, RFP-TGB1, and CFP-γb  in N. benthamiana leaves  at 3 dpi. Mixtures of A. tumefaciens containing αa-GFP, RFP-TGB1, and CFP-γb  were infiltrated into  NbPATs-silenced N. benthamiana leaves  or the non-silenced TRV:GUS control. Chloroplast  autofluorescence is displayed as a false pink color. Figures on the right show the normalized fluorescence intensities of GFP, RFP, CFP  and the chloroplast  autofluorescence channels along the white dashed lines in the merged images. Scale bars, 10 μm. Data information: In , representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"CFP: 
GFP: 
RFP: 
αa: 962673
TGB1: 962677
γb: 962674
GUS: 946149"
Figure legend: "D Co-IP assays of interactions between γb  and TGB1  in NbPAT15- and NbPAT21-silenced N. benthamiana leaves, TRV:GUS  and TRV:GUS combined with the  2-BP inhibitor provided positive and negative controls, respectively. The silenced leaves  were inoculated with BSMV3xFlag-TGB1/γb-GFP mixtures (RNAα + RNAβ3xFlag-TGB1 + RNAγγb-GFP) and harvested at 3 dpi. Total proteins were immunoprecipitated  with anti-Flag  beads. Input and IP  proteins were analyzed by Western blotting with anti-GFP or anti-Flag  antibodies. E Average amounts of RFP-TGB1  foci co-localizing with αa-GFP and CFP-γb  at the  chloroplast peripheries  shown in panel C (n = 12). Data information: In E), representative data are shown and three biological replicates had similar results. In (E), the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles, whiskers indicate minimum and maximum values, central band indicates the median. Letters in the chart denote statistically significant differences among groups according to the Duncan's multiple range test (p &lt; 0.05)."
NCBI gene IDs:
"Flag: 
GFP: 
RNAα: 962673
TGB1: 962677
RNAβ: 962679///962678///962677
RNAγ: 962674
γb: 962674
PAT15: 109211026
PAT21: 109240893
GUS: 946149"
Figure legend: "C Aqueous two-phase partitioning of γb PM localization. The microsomal  pellet from  BSMVγb-GFP-infected leaves  was obtained by ultracentrifugation at 100,000 g, and the  two-phase fractionation procedure was used to separate the plasma membrane (upper phase) from other  cellular membranes (lower phase). H+-ATPase is a PM  marker."
NCBI gene IDs:
"GFP: 
γb: 962674"
Figure legend: "D PM  associations of γb-GFP  and  γb3CS-GFP  in N. benthamiana protoplasts. The first and second columns show localization  of γb  at 3 dpi after infiltrations for transient expression of γb-GFP  and γb3CS-GFP. The third and fourth columns show γb localization in BSMVγb-GFP and BSMV3CS-GFP-infected leaves. PIP2A  provided a PM  marker (Nelson et al., 2007). Figures below the confocal  images show the normalized fluorescence intensities of the GFP  and mCherry  proteins along the white dashed lines in the merged images. Scale bars, 10 μm. Data information: In D, , representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"GFP: 
γb: 962674"
Figure legend: "F Western blot  detection of BSMV  accumulation in  N. benthamiana plants. A. tumefaciens  mixtures harboring RNAα  and RNAβmTGB2, along with RNAγ,  RNAγγb-ROP, or RNAγγb-mROP, were agroinfiltrated into  N. benthamiana leaves. Western blotting to detect virus accumulation at 3 dpi used anti-TGB1  or anti-CP  antibodies. Actin  provided loading controls."
NCBI gene IDs:
"ROP: 
RNAα: 962673
TGB2: 962678
RNAβ: 962679///962677///962678
RNAγ: 962674
γb: 962674"
Figure legend: "G Analysis of the effects of enhanced PM  association of γb   on  cell-to-cell virus movement   with the BSMV   duplex fluorescence (dfBSMV) reporter system. Mixtures of A. tumefaciens  containing dfBSMV, dfBSMVγb-GFP-ROP, and dfBSMVγb-GFP-mROP were infiltrated into N. benthamiana leaves. Infiltrated leaves  were observed by confocal microscope at 3 dpi. Scale bars, 100 μm. (n = 6). Data information: In G), representative data are shown and three biological replicates had similar results. In (G), the box boundaries indicate the upper (25th percentile) and lower (75th percentile) quartiles, whiskers indicate minimum and maximum values, central band indicates the median. Letters in the chart denote statistically significant differences among groups according to the Duncan's multiple range test (p &lt; 0.05)."
NCBI gene IDs:
"GFP: 
ROP: 
γb: 962674"
Figure legend: "B Co-IP  analyses to analyze interactions  of NbREM1 with γb  or γb3CS in  N. benthamiana leaf  tissues infiltrated with A. tumefaciens  mixtures containing movement-deficient BSMV plasmids (RNAα  + RNAγγb-GFP  or RNAγ3CS-GFP) and NbREM1-3xFlag. Total proteins were extracted at 3 dpi and immunoprecipitated with anti-Flag  beads. Input and immunoprecipitated  proteins (IP) were analyzed by Western blot  analyses with an anti-GFP  or anti-Flag  antibodies. Data information: In B, , representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"Flag: 
GFP: 
REM1: 
RNAα: 962673
RNAγ: 962674
γb: 962674"
Figure legend: "D Analysis of the cleaved 5(6)-carboxyfluorescein (CF) movement in N. benthamiana leaves. RNAα  + RNAγ/RNAγ3CS were co-infiltrated into transiently overexpressed  NbREM1 N. benthamiana leaves, and 1 μL CFDA was loaded onto the adaxial leaf  blade surface and incubated for 5 min followed by confocal  analysis at 3 dpi. The areas of dye diffusion are marked by dashed white circles. Scale bars, 100 μm. Data information: In D, representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"REM1: 
RNAα: 962673
RNAγ: 962674"
Figure legend: "H Aniline blue staining  of callose  in leaves with different treatments above the panel in NbPAT15 and NbPAT21 RNAi plants. Scale bars, 20 μm. Data information: In H), representative data are shown and three biological replicates had similar results."
NCBI gene IDs:
"PAT15: 109211026
PAT21: 109240893"
Figure legend: "(A) Immunoblot with anti-TG2 or anti-β-actin as loading control in in whole lysates from small intestine homogenates of CftrF508del/F508del and wild-type (CftrWT) mice (n=5 per group) and densitometric analysis of immunoblots. Mean ± SD of triplicates of independent pooled samples. **p&lt;0.01 (Student's t test). Data information: The blots are representative of one experiment for group of treatment."
NCBI gene IDs:
"Cftr: 12638"
Figure legend: "(B) Detection of NLRP3 expression (top) and caspase-1 cleavage (bottom) by immunoblot of whole lysates from small intestine homogenates of CftrWT and CftrF508del/F508del mice (n= 5) and densitometric analysis of immunoblots. Mean ± SD of triplicates of independent pooled samples. **p&lt;0.01 CftrWT vs CftrF508del/F508del (Student's t test). Data information: The blots are representative of one experiment for group of treatment."
NCBI gene IDs:
"Cftr: 12638"
Figure legend: "(C) Protein levels of IL-17A and IFN-γ from small intestine homogenates of CftrWT and CftrF508del/F508del (n= 10). Mean ± SD of triplicates of independent pooled samples. ***p&lt;0.001 CftrWT vs CftrF508del/F508del (Student's t test)."
NCBI gene IDs:
"Cftr: 12638"
Figure legend: "(D) IL-15 mRNA (left) and protein (right) levels in small intestine homogenates from CftrF508del/F508del, Cftr-/- or their CftrWT littermates (n= 10 per group). Mean ± SD of triplicates of independent pooled samples. **p&lt;0.01 CftrF508del/F508del vs CftrWT(FVB/129), or °°p&lt;0.01 Cftr-/- vs CftrWT(B6.129P2) (ANOVA, Bonferroni post hoc test)."
NCBI gene IDs:
"Cftr: 12638
IL-15: 16168"
Figure legend: "(E) IL-15 mRNA levels in small intestine homogenates from CftrWT mice or CftrF508del/F508del or TG-/-/CftrF508del/F508del or TG-/- mice (n= 10 per group). Mean ± SD of triplicates of independent pooled samples. ***p&lt;0.001 vs CftrWT, °°p&lt;0.01, ##p&lt;0.01 vs CftrF508del/F508del (ANOVA, Bonferroni post hoc test)."
NCBI gene IDs:
"Cftr: 12638
IL-15: 16168
TG: 21817"
Figure legend: "(F) Effects of 4 weeks of oral administration of gliadin on IL-15, IL-17A and IFN-γ protein levels in small intestine homogenates from CftrF508del/F508del and CftrWT mice (n =10 mice per group of treatment). Mean ± SD of triplicates of independent pooled samples. **p&lt;0.01, ***p&lt;0.001 (CftrF508del/F508del vs CftrWT mice prior gliadin challenge), °°°p&lt;0.001 (CftrF508del/F508del mice vs CftrF508del/F508del mice after gliadin challenge), (ANOVA, Bonferroni post hoc test)."
NCBI gene IDs:
"Cftr: 12638"
Figure legend: "(G-I) BALB/c mice (G) fed with a gluten-free diet for at least 3 generations, or (H) NOD or (I) NOD-DQ8 mice orally challenged with vehicle or gliadin for 4 weeks (5 mg/daily for one week and then 5 mg/daily thrice a week for 3 weeks). Representative traces of CFTR-dependent Cl- secretion measured by forskolin (Fsk)-induced increase of chloride current (Isc (μA/cm2)) in small intestines mounted in Ussing chambers; quantification of the peak CFTR inhibitor 172 (CFTRinh172)-sensitive Isc (∆Isc) in tissue samples (n=3 independent experiments). Mean±SD of samples assayed; **p&lt;0.01, ***p&lt;0.001 vs challenged with gliadin (Student's t test)."
NCBI gene IDs:
"DQ8: "
Figure legend: "Incubation of Caco-2 cells with P31-43, in the presence or absence of pre-treatment with TG2 inhibitor Z-DON, TG2-siRNA Immunoprecipitation in non-reducing and non-denaturing conditions of CFTR protein and immunoblot with anti-isopeptide glutamime-lysine and CFTR"
NCBI gene IDs:
"TG2: 7052"
Figure legend: "Caco-2 cells incubated with P31-43 in the presence or absence of VX-770 (G) IL-15 production (quantified by specific ELISA) in CFTR-WT Caco-2 cells treated or not with P31-43 (left) or in Caco-2CFTR-KO cells (right), in the presence or absence of VX-770. Mean ± SD of triplicates of independent experiments. ***p&lt;0.001 vs untreated and °°°p&lt;0.001 vs CFTR-WT Caco-2 cells treated with P31-43+VX-770 (ANOVA, Bonferroni post hoc test). **p&lt;0.01 vs Caco-2 CFTR WT (Student's t-test)."
NCBI gene IDs:
"CFTR: 1080"
Figure legend: "Caco-2 cells incubated with P31-43 in the presence or absence of VX-770 (H) Caco-2 or Caco-2CFTR-KO cells in the presence or absence of pre-treatment with VX-770. Immunoblot of phERK1\2 or β-actin (left); densitometric analysis of protein levels (right). Mean ± SD of triplicates of independent experiments. *p&lt;0.05 (Student's t-test). Data information: The blots are representative of one experiment for group of treatment."
NCBI gene IDs:
"CFTR: 1080"
Figure legend: "BALB/c mice fed with a gluten-free diet for at least 3 generations, orally challenged with vehicle or gliadin for 4 weeks (5 mg/daily for one week and then 5 mg/daily thrice a week for 3 weeks) in the presence or absence of intraperitoneal VX-770 administered 15 minutes prior gliadin challenge (n=10 mice per group of treatment). Transcript level of Rorc and Tbet (G) from small intestine homogenates. Mean ± SD of triplicates of independent pooled samples. ***p&lt;0.001 or ****p&lt;0.0001 gliadin vs VX-770+gliadin (ANOVA, Bonferroni post hoc test)."
NCBI gene IDs:
"Rorc: 19885
Tbet: 57765"
Figure legend: "(L) IFN-γ release (ELISA) into culture supernatants by PBMC from 3 celiac patients cultured in the lower compartment of a bidimensional co-culture model upon a 24 h challenge of confluent Caco-2CFTR-KO cells in the upper compartment with a combination of P31-43 and P57-68 in the presence or absence of VX-770, as in J and K. *p&lt;0.05, medium vs P57-68/P31-43 combination (n=3) (ANOVA, Bonferroni post hoc test)."
NCBI gene IDs:
"CFTR: 1080"
Figure legend: " (A) Immunofluorescence analysis of HeLa cells transfected with either a non-target dsRNA (NT) or one of the three siRNAs targeting LETM1 (siR1, siR2 or siR3) and labeled with anti-TOM20 antibody. In siR1 treated cells, the mitochondria formed a 'honeycomb' of swollen distinct organelles; siR3 resulted in giant organelles with a central region distinguished by reduced TOM20; siR2, produced relatively little swelling and the mitochondrial network was generally well preserved. The pronounced swelling induced by siR1 significantly increased circularity (p = 2.32E-58) (ImageJ analysis), siR1 circularity = 0.683 ±0.012 compared with 0.414 ±0.008 for the NT; where 1 = a perfect circle. Over 150 mitochondria were quantified in each cell type; data are means ± SEM. Scale bar represents 12 µm in the main images and 4 µm in offset magnification "
NCBI gene IDs:
"LETM1: 3954"
Figure legend: " (D) Representative immunoblots for the OXPHOS proteins NDUFB8 and COII of HeLa cells transfected with NT or siLETM1 (siR1, siR2 or siR3). Vinculin and GAPDH are shown as loading controls. The mean relative abundances for respiratory subunits COII and NDUFB8 are shown beneath the blots. Data are expressed as mean ± SEM of n=8 independent experiments "
NCBI gene IDs:
"LETM1: 3954"
Figure legend: " (E) Mitochondrial oxygen consumption rate (OCR) measured using a Seahorse flux analyzer before (basal) and after the addition (maximal) of the uncoupler FCCP, in HeLa cells treated with NT or siLETM1 (siR1 or siR2). Data are means ± SEM of n=5 experiments "
NCBI gene IDs:
"LETM1: 3954"