EMBO/SourceData huggingface dataset:
Figure legend: "C-F RT-qPCR analysis of mRNA from ribosomal  protein fractions for control (black) and pths KD (gray) transcripts from S2R+ cells. RNA was isolated individually from fractions and pooled into five categories: RNP, 40S/60S, monosome, low polysome (di- and trisome), and high polysome (remaining fractions). The data were plotted relative to the amount present in the monosome fraction for each transcript. The mRNA levels of subunits of mitochondrial complexes (C) I, (D) III and (E) V were reduced in low and high polysome fractions in pths KD cells compared to the control cells, while (F) mRNA levels of GAPDH in the same fractions, used as an internal control, were not changed (n=3 independent biological replicates for control and pths KD). For (C,E-F) low polysome fraction for control vs. pths KD: (C) p=0.057, (D) p=0.0039, (E) p=0.09, (F) p=0.5. For (C,E-F) high polysome fraction for control vs. pths KD: (C) p=0.039, (D) p=0.046, (E) p=0.041, (F) p=0.66. G-I Western blots and their quantifications of protein extracts from control and pths KD S2R+ probed with (G)  MT-ND1 (for mitochondrial complex I) n=4, p&lt;0.0001), (H) ATPsynt-β (for mitochondrial complex V) n=3 p=0.034 and (I)  tubulin β antibodies p=0.56) (n=3). Profilin serves as loading control. N=biological replicates. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. Unpaired t-tests (C-F, G-I)."
NCBI gene IDs:
"GAPDH: 
pths: 35379"
Figure legend: "B The Oxygen Consumption Rate (OCR, pmols  O2/min) assessed as a representative parameter of OxPhos in control and pths-KD S2R+ cells by a Seahorse efflux assay. n≥3 independent biological experiments each with n&gt;6 technical replicate. Data information: Mean±SEM, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Unpaired t-tests"
NCBI gene IDs:
"pths: 35379"
Figure legend: "Confocal images of germband macrophages in control embryos and those expressing different RNAis against mitochondrial OxPhos Complex III (or an RNAi against Complex V. Data information: show Stage 12 embryos.  Complex III RNAis target Cyt-c1, UQCR-cp1-2, for Complex V, ATP synthase F1F0. Scale bars: 50µm"
NCBI gene IDs:
"Cyt-c1: 38612
UQCR-cp1: 41800"
Figure legend: "quantification of germband macrophages in control embryos and those expressing different RNAis against mitochondrial OxPhos Complex III (or an RNAi against Complex V. For (H) control (n=34 embryos) vs Complex III RNAi 1 (n=20) p=0.0001; vs. Complex III RNAi 2 (n=18) p=0.027; vs. Complex III  RNAi 3 (n=16) p&lt;0.0001; vs. Complex V RNAi (n=14) p&lt;0.0001. Data information: show Stage 12 embryos. Complex III  RNAis target Cyt-c1, UQCR-cp1-2, for  Complex V,  ATP synthase F1F0. Mean±SEM, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. one-way ANOVA with Tukey's (H)."
NCBI gene IDs:
"Cyt-c1: 38612
UQCR-cp1: 41800"
Figure legend: "I Single plane  confocal microscope  image during germband entry from control or atosPBG embryos, or those expressing  pths-RNAi or CV-DN in macrophages. Antibodies against S293-phosphorylated inactivated  Pyruvate Dehydrogenase (pPDH, green) or total PDH (magenta) in  macrophages (red). Higher pPDH levels are usually found when  ATP/ADP  levels are high and input into the TCA cycle is being downregulated Data information: show Stage 12 embryos. Scale bars: 10µm"
NCBI gene IDs:
"atos: 36243
pths: 35379"
Figure legend: "J Quantification of normalized pPDH/PDH levels calculated from fluorescence intensities in macrophages from the genotypes in (I) during initial germband  invasion. The pPDH/PDH ratio is significantly reduced, arguing that decreased function of CV, Atos  or Pths in macrophages results in lower cellular ATP/ADP ratios compared to the control. N=3 independent experiments. Ctrl 1 (n=10 embryos) vs CV-DN  (n=9) and vs.  atos  mutant (n=13) both p=0.0002; Ctrl 2 (n=7 embryos) vs pths RNAi (n=8) p=0.0001.*** shown above columns in J are for comparison to relevant control. Data information: Mean±SEM, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001. Unpaired t-tests"
NCBI gene IDs:
"atos: 36243
Atos: 36243
Pths: 35379
pths: 35379"
Figure legend: "A Single plane confocal microscope  image during germband entry from control or atosPBG embryos, or those expressing pths, GR/HPR, and LKR/SDH in macrophages (mac&gt;). Embryos were stained  for antibodies against S293-phosphorylated inactivated Pyruvate Dehydrogenase (pPDH, green) or total PDH (magenta) in macrophages (red). Higher pPDH/PDH ratios are consistent with higher  ATP/ADP  levels. Data information: Scale bars: 10µm"
NCBI gene IDs:
"atos: 36243
pths: 35379
GR/HPR: 35347
LKR/SDH: 34064"
Figure legend: "B Quantification of normalized pPDH/PDH levels calculated from fluorescence intensities in macrophages from the genotypes in (A) during initial germband invasion. The pPDH/PDH ratio is significantly increased in  atosPBG embryos expressing either  pths, GR/HPR, or LKR/SDH in macrophages compared to the atosPBG embryos. This argues that the decreased Atos  function in atosPBG macrophages, resulting in lower cellular ATP/ADP ratios, was restored by expressing either its targets or murine orthologs. N=3 independent experiments. Control (n=9 embryos) vs.  atos mutant (n=10) p&lt;0.0001. Control vs. atos mutant rescued with mac&gt;pths (n=10) p=0.77; rescued with  mac&gt;GRHPR (n=9) p=0.48; rescued with mac&gt;LKRSDH (n=10) p=0.012. For  atos mutant vs. atos rescued with  mac&gt;pths or  mac&gt;GRHPR p&lt;0.0001. vs. atos rescued with  mac&gt;LKRSDH p=0.0014. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. One-way ANOVA with Tukey"
NCBI gene IDs:
"atos: 36243
Atos: 36243
pths: 35379
GR/HPR: 35347
GRHPR: 35347
LKR/SDH: 34064
LKRSDH: 34064"
Figure legend: "C-D (C) Confocal images or (D) quantification of the  macrophages in germband  in Stage 12 embryos from the control, atosPBG, and atosPBG expressing atos itself or  GR/HPR, or LKR/SDH in macrophages. Germband edge: dotted white line. For (D) control (n=29 embryos) vs. atos mutant (n=19) p&lt;0.0001. Control vs. atos  mutant rescued with  mac&gt;atos (n=27 embryos) p=0.99; rescued with  mac&gt;GRHPR (n=28) p=0.29; rescued with mac&gt;LKRSDH (n=20) p=0.036. atos  mutant vs. atos  rescued with   mac&gt;atos or mac&gt;GRHPR p&lt;0.0001; rescued with mac&gt;LKRSDH p=0.0134. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. One-way ANOVA with Tukey Scale bars: 50µm"
NCBI gene IDs:
"atos: 36243
GR/HPR: 35347
GRHPR: 35347
LKR/SDH: 34064
LKRSDH: 34064"
Figure legend: "E Single plane confocal microscope images during germband entry from control or atosPBG embryos, or those expressing mammalian orthologs mFAM214A or B (FAMA-B) in  macrophages  (mac&gt;). Embryos were stained for antibodies against S293-phosphorylated inactivated Pyruvate Dehydrogenase (pPDH, green) or total PDH (magenta) in macrophages  (red). Data information: Scale bars: 10µm"
NCBI gene IDs:
"atos: 36243
FAM214A: 235493
FAMA-B: 230088"
Figure legend: "F Quantification of normalized pPDH/PDH levels measured from fluorescence intensities in  macrophages from the genotypes in (E) during initial germband invasion. The pPDH/PDH ratio is significantly increased in  atosPBG embryos  expressing either mammalian ortholog mFAM214A or B in macrophages compared to the  atosPBG embryos. Results from three independent experiments. Control (n=10 embryos) vs. atos mutant (n=11) p&lt;0.0001. Control vs. atos  mutant rescued with mac-mFAMA (n=14 embryos) p=0.06; rescued with mac-mFAMB (n=12) p=0.13. atos mutant vs. atos mutant rescued with mac-mFAMA p=0.0025 or  mac-mFAMB p=0.0019. Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. One-way ANOVA with Tukey"
NCBI gene IDs:
"atos: 36243
FAM214A: 235493
FAMA: 235493
FAMB: 230088"
Figure legend: "Cellular metabolites were measured by  LC-MS-based untargeted metabolomics from extracts of Stage 11 embryos; biological replicates for control n=5, for atosPBG n=7. (B) Normalized ATP/ADP ratio values are decreased in atosPBG compared to control embryos  (p=0.028). Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. unpaired t-test"
NCBI gene IDs:
"atos: 36243"
Figure legend: "Cellular metabolites were measured by LC-MS-based untargeted metabolomics from extracts of Stage 11 embryos; biological replicates for control n=5, for atosPBG n=7. Heatmap of non-targeted metabolites in atosPBG compared to wild-type embryos shown with average log2fold change (FC) in the non-targeted  LC-MS/MS analysis reveals (C) an increase in atosPBG in substrates of the dGR/HPR  enzyme, including 4-hydroxy α-ketoglutarate and hydroxyproline (HLP) and a smaller decrease in its products, glycolate and glycerate, (D) a significant increase in some dipeptides including those containing  hydroxyproline. Data information: Values in heat maps are obtained from untargeted  metabolomic analysis, unpaired t-test"
NCBI gene IDs:
"atos: 36243"
Figure legend: "Cellular metabolites were measured by LC-MS-based untargeted metabolomics  from extracts of Stage 11 embryos; biological replicates for control n=5, for  atosPBG n=7. (E) Quantification shows an increase in atosPBG in the pyruvate/glucose ratio (p=0.035), but none for the  lactate/glucose ratio (p=0.65). Data information: Mean±SEM, ns=p&gt;0.05, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001. unpaired t-test"
NCBI gene IDs:
"atos: 36243"
Figure legend: "Cellular metabolites were measured by LC-MS-based untargeted metabolomics  from extracts of Stage 11 embryos; biological replicates for control n=5, for  atosPBG n=7. Heatmap of non-targeted metabolites in atosPBG compared to wild-type embryos  shown with average log2fold change (FC) in the non-targeted  LC-MS/MS analysis (F) We observe increases in atosPBG in intermediates of mitochondrial fatty acid β-oxidation (FAO), including different carnitine-conjugated lipids, and (F-G) a significant increase in ketone body substituents compared to the control. Data information: Values in heat maps are obtained from untargeted metabolomic analysis, unpaired t-test"
NCBI gene IDs:
"atos: 36243"
Figure legend: "WM983 and UACC257 human melanoma cells were transfected with Perfringolysin O (PFO)-mCherry D4H cholesterol sensor and plated on glass coverslips. Cells were exposed to UV (0 or 175 J/m2) and incubated for 0, 2 or 5 weeks as indicated. (H) Representative cell images. (I) Fluorescence intensity from cells analyzed in (H) was measured and quantified using LASX analysis software (N≥8 biological replicates); data displayed are mean±SEM. Data was analyzed using 1-way ANOVA with multiple comparisons (WM983, p &lt; 0.001; UACC257 p &gt; 0.05). **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001."
NCBI gene IDs:
"mCherry: 
Perfringolysin O: 69447922
PFO: 69447922"
Figure legend: "O1C78 WM983 cells expressing the PFO-mCherry D4H cholesterol sensor were examined as in panels H and I (N≥8 biological replicates); data displayed are mean±SEM."
NCBI gene IDs:
"mCherry: 
PFO: 69447922"
Figure legend: "(D) SOCE in primary patient samples exhibiting NRAS (8 of 27 samples), BRAF (6 of 27 samples), TERT promoter (6 of 27 samples), NF1 (5 of 27 samples), and CDKN2A (5 of 27 samples) alterations were compared to SOCE in samples that expressed WT or the unaltered gene. Data displayed as mean±SEM."
NCBI gene IDs:
"BRAF: 673
CDKN2A: 1029
NF1: 4763
NRAS: 4893
TERT: 7015"
Figure legend: "Expression of ERα in control (shCtrl) and knockdown (shERα) BM67 cells was determined by western blotting (β-actin served as a loading control). Gene expression was determined using polysome-profiling which quantifies both total mRNA and efficiently translated polysome-associated mRNA (n = 3)."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Distributions (quantified by kernel density estimation) of p-values for differential expression (shERα vs. shCtrl BM67 cells) using data from polysome-associated mRNA (orange), cytosolic mRNA (purple) or from analysis of changes in translational efficiency (i.e. changes in polysome-associated mRNA not paralleled by changes in cytosolic mRNA) leading to altered protein levels (red; a higher density of low p-values indicates a higher frequency of changes in gene expression)."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Scatterplot of polysome-associated mRNA vs. cytosolic mRNA log2 fold-changes (shERα vs. shCtrl). Areas of the plot are colored according to the density of data points (genes; dark blue corresponds to areas with many genes"
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Targets from each mode of regulation (cf. Fig 1E) were selected for validation by Nanostring. Shown is a scatterplot of Nanostring quantification of polysome-associated mRNA vs. cytosolic mRNA log2 fold-change (shERα vs. shCtrl; n = 3). Genes are colored according to their identified mode of regulation (i.e. from Fig 1E). Confidence ellipses (based on Nanostring data, level 0.7) are overlaid for each mode of regulation. Selected genes from each mode of regulation are indicated."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Levels of indicated proteins from shERα and control BM67 cells were determined by western blotting with their quantifications. β-actin served as loading control. Graphs represent mean ± standard deviation (n = 3-5 as indicated). Protein expression between groups were compared using two-sided paired Student's t tests."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "nanoCAGE sequencing was applied to determine transcription start sites in shERα BM67 cells. The number of detected transcription start sites (peaks) and RefSeq transcripts when sampling increasing number of RNAseq reads are indicated to evaluate the complexity of nanoCAGE RNAseq libraries."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Volcano plot of ERα dependent miRNA expression in BM67 cells quantified by small RNAseq. Down- and up-regulated miRNAs (FDR&lt;0.2 and fold-change &gt; 1.25) are colored in blue and red, respectively."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Validation of miRNA expression using qPCR. Normalized expression (mean ± standard deviation; n = 3) (B) and expression fold change (shERα vs. shCtrl) obtained from small RNAseq vs. qPCR (C)."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Percentage of miRNA among small RNAs in shERα and shCtrl BM67 cells (mean ± standard deviation; n = 3)."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Assessment of the relationship between presence of miRNA target sites and translational offsetting. (E) For visualization, mRNAs were assigned angles describing their mode of regulation such that mRNAs with congruently modulated total and polysome-associated mRNA obtain an angle of ~45 degrees, whereas offset mRNAs are assigned an angle of ~0 degrees. (F) The distributions (quantified by kernel density estimation) of such angles were then compared between mRNAs whose levels were induced upon ERα depletion, which were also targets of each suppressed miRNAs separately (blue lines); and the set of background transcripts (mRNAs induced upon ERα depletion but not targets of suppressed miRNAs; black). (G) For such subsets of miRNA targets (i.e. from F), the relative number of offset and non-offset mRNAs (as determined by anota2seq analysis) was also compared to the background (same as in F) using Fisher's exact test; and the resulting adjusted p-values (FDRs; across all assessed miRNAs) were visualized as a histogram. (H-I) A similar assessment as in (F-G) but for suppressed mRNAs and induced miRNAs. (J) A similar assessment as in (H) but for randomly selected groups of 10 miRNAs (gray lines) compared to background (black) and targets of all induced miRNA (red; i.e. from H)."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "For each codon, the average frequency (per thousand) is compared between transcripts induced but offset vs non-offset (i.e. abundance mode of regulation) upon ERα depletion in BM67 cells. Codons for the same amino acid are connected by a gray line. Heatmap of standardized residuals from a chi-squared contingency table test. Shown in red and blue are cells with counts significantly higher and lower, respectively, than expected counts under the null hypothesis (i.e. independence between mode for regulation of gene expression and codon composition). A correspondence analysis for average codon frequency among transcripts annotated to each GO term. Each gray dot corresponds to one GO term. The gene expression modes observed upon ERα depletion (Fig 1E) were then projected into the same dimensions based on the average codon frequencies of included transcripts. A correspondence analysis for codon frequency in each regulated mRNA (from Fig 1E). Each dot represents one mRNA and is colored according to its mode of regulation. Codons identified as over-represented among translationally offset mRNAs are also indicated. Unsupervised clustering of gene level codon usage normalized by amino acid counts. All regulated mRNAs (from Fig 1E) are shown in rows and all codons in columns. Codons identified as over-represented among mRNAs whose levels were induced but offset or suppressed but offset are indicated in light and dark blue, respectively."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "Immunoblotting of BM67 cell extract for ELP3 after stable shRNA-mediated ERα knockdown in BM67 cells. Graphs represent mean ± standard deviation of n=3."
NCBI gene IDs:
"ERα: 13982"
Figure legend: "qPCR analysis and immunoblotting of BM67 cell extract for indicated proteins with its quantitation following deletion of Esr1 gene by CRISPR-Cas9 technology. Graphs represent mean ± standard deviation of n=5."
NCBI gene IDs:
"Esr1: 13982"
Figure legend: "qPCR analysis and immunoblotting of BM67 cell extract for ELP3 and DEK with its quantitation following deletion of Elp3 gene by CRISPR-Cas9 technology. Graphs represent mean ± standard deviation of n=4."
NCBI gene IDs:
"Elp3: 74195"
Figure legend: "Immunoblotting and qPCR analysis comparing cell extracts obtained from BM67 cells transduced with pLentiCRISPRV2 empty vector, Elp3-null BM67 cells and Elp3-null BM67 cells overexpressing ELP3 protein containing silent mutations in the sgRNA binding site. Graphs represent mean ± standard deviation of n=3-4."
NCBI gene IDs:
"Elp3: 74195"
Figure legend: "Boxplots (plotted as in Fig 3B) of gene expression of ELP3, ALKBH8 and CTU2 upon treatment with 17β-E2 and/or ICI-182780 in MCF7 cells (extracted from (Wardell et al, 2012; Data Ref: McDonnell et al, 2012)). Wilcoxon-Mann-Whitney tests (two-sided) were used to assess differences between conditions (n = 10) and a global Bonferroni adjustment was applied on p-values."
NCBI gene IDs:
"ALKBH8: 91801
CTU2: 348180
ELP3: 55140"
Figure legend: "ChIP-seq reveals ER binding sites proximal to the ELP3 gene. Each track represents a distinct ChIP-seq dataset."
NCBI gene IDs:
"ELP3: 55140"
Figure legend: "Immunoblotting of MCF-7 cell extract for indicated proteins following deletion of ELP3 gene by CRISPR-Cas9 technology."
NCBI gene IDs:
"ELP3: 55140"
Figure legend: "Quantification of mcm5S2-U, mcm5U and cm5U levels in ELP3-null MCF-7 cells and in MCF-7 cells treated with 100 nM ICI-182780 for 72 hours versus their respective controls by liquid chromatography-mass spectrometry (LC-MS) analysis. The MCF-7 cells were grown in phenol red-free RPMI media supplemented with 5% charcoal-stripped serum for 24 hours prior to ICI-182780 treatment. Graphs represent mean ± SD of n=4. Data were analyzed using two-sided paired Student's t tests. Vh, Vehicle; ICI, ICI-182780."
NCBI gene IDs:
"ELP3: 55140"
Figure legend: "C Quantitative analysis of lysosomal movement from kymographs. Results were expressed as percent of total lysosomes (N = 44, 49, and 16 neurons for shSc, shLAMTOR1, and shLAMTOR+rLAMTOR1 respectively from 3-10 independent experiments). Data information: Data with error bars are represented as means ± SEM. Statistical significance was assessed by two-way ANOVA with Tukey's *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, as compared to shSc; #P &lt; 0.05, ###P &lt; 0.001, as compared to shLAMTOR1 n.s., not significant."
NCBI gene IDs:
"LAMTOR: 66508
LAMTOR1: 66508"
Figure legend: "G FRAP  analysis of dendritic lysosome movement. Quantitative analysis for F (N = 7, 7, and 9  neurons for shSc, shLAMTOR1, and shLAMTOR+rLAMTOR1, respectively, from 3 independent experiments). Data information: Data with error bars are represented as means ± SEM. Statistical significance was assessed by Dunnett's post-test *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, as compared to shSc; #P &lt; 0.05, ###P &lt; 0.001, as compared to shLAMTOR1 ; n.s., not significant."
NCBI gene IDs:
"LAMTOR: 66508
LAMTOR1: 66508"
Figure legend: "A Representative kymographs of  LysoTracker-labeled  lysosomes in  proximal dendrites  of  hippocampal neurons  infected with scrambled shRNA (shSc), LAMTOR2  shRNA (shLAMTOR2), or Raptor shRNA (shRaptor). Scale bar, 5 µm. B Quantitative analysis of lysosomal movement  from kymographs (N = 44, 24, and 19 neurons for shSc, shLAMTOR2, and shRaptor, respectively, from 3-10 independent experiments). Data information: Data with error bars are represented as means ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak's post-test ***P &lt; 0.001 as compared to shSc; n.s., not significant."
NCBI gene IDs:
"LAMTOR2: 83409
Raptor: 74370"
Figure legend: "C, D FRAP  analysis of dendritic lysosomal movement in Raptor  KD neurons. C Representative images of a  neuron at 0 and 4 min after photobleaching. D Quantification of the  lysosomes in C undergoing retrograde (red) or  anterograde (cyan) transport (N = 7 and 8 neurons for shSc and shRaptor, respectively, from 3 independent experiments). Data information: Data with error bars are represented as means ± SEM. Statistical significance was assessed by Mann-Whitney U test ***P &lt; 0.001 as compared to shSc; n.s., not significant."
NCBI gene IDs:
"Raptor: 74370"
Figure legend: "E LAMTOR1 KD did not affect trafficking of vesicles labeled with Alexa 594-conjugated transferrin (red, Tf  594) in dendrites. Shown are dendritic segments (upper) and kymographs (lower). Scale bar, 5 µm. F Quantitative analysis of  vesicular movement from  kymographs (N = 10 neurons from 3 independent experiments). Data information: Data with error bars are represented as means ± SEM. Statistical significance was assessed by Mann-Whitney U test ***P &lt; 0.001 as compared to shSc; n.s., not significant."
NCBI gene IDs:
"LAMTOR1: 66508"
Figure legend: "A Quantification of the percent of lysosomes moving in the anterograde or retrograde direction in neurons infected with AAV  expressing either LAMTOR1 shRNA (shLAMTOR1) or scrambled shRNA (shSc) and Accell lyspersin siRNA or control siRNA. Neurons were imaged with LysoTracker  to visualize lysosomal  trafficking in dendrites. N = 17-31  neurons  from 3-6 independent experiments. Data information: Data with error bars are represented as means ± SEM. ***P &lt; 0.001 compared with shSc or shSc/Accell siControl; ##P &lt; 0.01, ###P &lt; 0.001 compared with shLAMTOR1 or shLAMTOR1/Accell siControl; &amp;&amp;P &lt; 0.01, &amp;&amp;&amp;P &lt; 0.001 compared with shSc/Accell siLyspersin; n.s., not significant; two-way ANOVA with Tukey's post-test."
NCBI gene IDs:
"lyspersin: 71923
Lyspersin: 71923
LAMTOR1: 66508"