EMBO/SourceData huggingface dataset:
Figure legend: "(E) Volcano plots of proteins in close proximity to Apl5-TurboID versus control cells in a GAL1pr-Vps41-GFP-Fis1 background, from a label-free proteomics analysis of streptavidin-biotin pulldowns with cells grown in YPG. The logarithmic ratios of protein intensities are plotted against negative logarithmic P values of two tailed Student's t-test, equal variance, performed from n = 3 independent experiments. The red dashed line (significance, 0.001) separates specifically identified proteins (top right portion of plot) from background. Selected top hits are indicated with black dots"
NCBI gene IDs:
"TurboID: 
Apl5: 855906"
Figure legend: "(F) Co-localization of C-terminally GFP-tagged Apl5 and C-terminally mCherry-tagged Sec7 in wt and age2Δ background. Cells were grown at 30°C and kept in logarithmic phase. Scale bar, 5 µm. Scale bar in inset, 2 µm. (G) Quantification of co-localization from (F) normalized per cell. Number of co-localizing dots was determined and normalized to the number of co-localization events per cell. Bars show the mean number of co-localizing dots per cell ± standard deviation (n ≥ 60). "
NCBI gene IDs:
"age2: 854767"
Figure legend: "(A, B) AP-3 sorting defects in strains overexpressing Gcs1 (A) or Age2 (B). The AP-3 reporter GNS was localized relative to FM4-64 stained vacuoles in the indicated strains. Scale bar, 5 µm."
NCBI gene IDs:
"Age2: 854767
Gcs1: 851372"
Figure legend: "(E, F) AP-3 sorting defects in GAP-dead mutants of Gcs1 (E) and Age2 (F). The AP-3 reporter GNS was localized relative to FM4-64 stained vacuoles in the indicated strains. Scale bar, 5 µm."
NCBI gene IDs:
"Age2: 854767
Gcs1: 851372"
Figure legend: "(G) Localization of AP-3 in GAP-dead mutants of Gcs1 and Age2. Localization of C-terminally GFP-tagged Apl5 relative to FM4-64 stained vacuoles in the indicated strains. Scale bar, 5 µm. (H) Quantification of Apl5 dots in (G). Apl5-positve dots were counted and averaged to the number of dots per cell. Box boundaries indicate 25% and 75% of the dataset. The middle line indicates the median and the small square the mean. "
NCBI gene IDs:
"Age2: 854767
Gcs1: 851372"
Figure legend: "(A-C) The relative levels of (A) canalicular membrane (BA) transporter Bsep, Mrp2, Mdr1b, Mdr2, Abcg5 and Abcg8 mRNA transcripts, (B) basolateral membrane BA transporter Mrp3, Mrp4, Ostβ, Ntcp and Oatp1a1 mRNA transcripts, and (C) BA synthetic enzyme Cyp7a1, Cyp7b1, Cyp8b1, Cyp27a1, and Cyp2c70 mRNA transcripts. Wild type, wild type mice, n=6; Heterozygote, Sema7aR145W heterozygous mice, n=5; Homozygote, Sema7aR145W homozygous mice, n=7. Data are shown as means ± SD. *P &lt; 0.05 versus the WT mice, #P &lt; 0.05 versus the heterozygous mutant mice. The data were analyzed by the independent-samples Student's t-test and the Mann-Whitney U test."
NCBI gene IDs:
"Bsep: 27413
Mdr1b: 18669
Mdr2: 18670
Mrp2: 12780
Mrp3: 76408
Mrp4: 239273
Abcg5: 27409
Abcg8: 67470
Cyp27a1: 104086
Cyp2c70: 226105
Cyp7a1: 13122
Cyp7b1: 13123
Cyp8b1: 13124
Sema7a: 20361
Ntcp: 20493
Ostβ: 330962
Oatp1a1: 28248"
Figure legend: "(D) Western blot analysis of the relative levels of Bsep, Mrp2, Mdr2, Mrp3, Mrp4, Ostα/β, Ntcp, Cyp7a1 and Cyp8b1 protein expression in mouse livers. Contrary to the mRNA alterations, the levels of canalicular membrane Bsep and Mrp2 proteins were dramatically reduced in homozygous mouse livers, suggesting that the decreased these proteins were might be mediated by post-translational regulation in the livers of Sema7aR145W mutant mice. Wild type, wild type mice, n=4; Heterozygote, Sema7aR145W heterozygous mice, n=5; Homozygote, Sema7aR145W homozygous mice, n=5. *P &lt; 0.05 versus the WT mice, #P &lt; 0.05 versus the heterozygous mutant mice. The data were analyzed by the independent-samples Student's t-test and the Mann-Whitney U test."
NCBI gene IDs:
"Sema7a: 20361"
Figure legend: "(G) Multiplex IF analyses revealed that transfection with the SEMA7A_WT or SEMA7A_R148W construct decreased the levels of canalicular membrane Bsep and Mrp2 expression in mouse primary hepatocytes in collagen sandwich cultures in a dose-dependent manner (0, 0.2, 1.0 μg per well of 6-well plate; 1.5 ml culture medium per well). Scale bar, 50 µm. Together, the Sema7aR145W mutation can reduce hepatic Bsep and Mrp2 protein expression and lead to intrahepatic BA accumulation and cholestasis (Appendix Table S16). Subsequently, cholestasis triggered an adaptive response in the liver by down-regulating the expression of BA synthetic enzymes of Cyp7a1 and Cyp8b1 and up-regulating the expression of BA efflux transporters Mrp3, Mrp4 and Ostα/β."
NCBI gene IDs:
"SEMA7A: 20361
Sema7a: 20361"
Figure legend: "A Confocal microscopy images representing the localization of PCDH7. U2OS cells expressing full‐length (WT) PCDH7 at mitosis (top) and interphase (middle). Cytoplasmic deleted (CytΔ) PCDH7‐expressing cells at mitosis (middle) and interphase (bottom). PCDH7, green; DNA, blue. Scale bar, 20 μm.B Quantification of PCDH7::GFP (WT and CytΔ) protein localization on the cell surface in mitosis [WT (n = 11), CytΔ (n = 6), red] and interphase [WT (n = 25), CytΔ (n = 20), blue]. Interphase cytΔ PCDH7 localizes to the cell surface more than WT (full length) PCDH7 (insets). Bars show mean ± SEM."
NCBI gene IDs:
"PCDH7: 5099"
Figure legend: "A PCDH7 antibody detects a single band of ˜110 kDa in luciferase RNAi (luc), PCDH1 RNAi HeLa cell lysates, but this band is not detectable in PCDH7 and PCDH1 and PCDH7 RNAi cells. α‐tubulin was used as a loading control (left). HeLa Kyoto cells stably expressing PCDH7‐LAP‐tagged BAC transgenes treated with luciferase RNAi (control) and 150 nM (1×) and 300 nM (2×) PCDH1 esiRNA and blotted against anti‐GFP, anti‐PCDH1, and anti‐actin antibodies. PCDH1 bands were reduced in PCDH1 RNAi cells, whereas mouse GFP::PCDH1 was not affected by the esiRNA treatment (right)."
NCBI gene IDs:
"luciferase: 
PCDH1: 75599
PCDH1: 5097
PCDH7: 5099"
Figure legend: "B Rounding pressure plots of MYH9 (positive control, n = 7), luc (negative control, n = 12), PCDH7 (n = 11), PCDH1 (n = 12), and PCDH1 and PCDH7 (n = 11) RNAi cells. Bars show mean ± SEM (left). Rounding pressure plots HeLa Kyoto cells stably expressing PCDH7‐LAP‐tagged BAC transgenes treated with MYH9 (positive control, n = 7), luc (negative control, n = 14), PCDH1 (n = 13) esiRNA. *P 0.05; **P 0.01; ***P 0.001; ns, P &gt; 0.05 (non‐significant)."
NCBI gene IDs:
"luc: 
MYH9: 4627
PCDH1: 75599
PCDH1: 5097
PCDH7: 5099"
Figure legend: "(B) Cells expressing fluorescently-tagged α2-tubulin (atb2) were imaged every 5 seconds and the dwell time of ~100 individual iMTs recorded within the final 1.1 µm of the cell for each strain. Red bars signify the mean"
NCBI gene IDs:
"atb2: 2540936
α2-tubulin: 2540936"
Figure legend: "(C) Kymographs showing fluorescently-tagged Klp5/Klp6 (Kinesin) co-imaged with fluorescently-tagged microtubules (MT) in the presence (top panels) and absence (bottom panels) of Mcp1. Banding on MT, caused by unequal incorporation of fluorescence, illustrates the force exerted on the MT by continued growth into the cell cortex. Dashed yellow line indicates the cell end"
NCBI gene IDs:
"Mcp1: 2542755"
Figure legend: "(D) MT growth speed was calculated from kymographs from control (n=16) and ∆mcp1 cells (n=11), Klp5/Klp6 walk speed was calculated from multiple individual runs on the MT lattice in control (n=44) and ∆mcp1 cells (n=32)"
NCBI gene IDs:
"mcp1: 2542755"
Figure legend: "(E) Average intensity of Klp5/Klp6 at the plus ends of iMTs from multiple kymographs of control (n=19) or ∆mcp1 cells (n=14)"
NCBI gene IDs:
"mcp1: 2542755"
Figure legend: "(F) Mixing experiment to compare fluorescently-tagged Klp5/Klp6 levels between cells either expressing (blue, closed arrowheads) or deleted (red, open arrowhead) for Mcp1 distinguished by the absence of fluorescently-tagged nuclear envelope protein Cut11 (left panel). Scale bar, 5µm. Box plot (right panel) shows quantitated fluorescence values for nuclear levels of Klp5/Klp6 in control (n=44) and ∆mcp1 cells (n=45) and at the MT plus end in control (n=64) and ∆mcp1  cells (n=35) prior to shrinkage"
NCBI gene IDs:
"mcp1: 2542755
Mcp1: 2542755"
Figure legend: "(B) Kymographs showing fluorescently-tagged MTs co-imaged with fluorescently-tagged Tea2 kinesin in control cells (top panels) or in the absence of Klp6 (middle panels) or Mcp1 (bottom panels). Dashed yellow line indicates the cell end"
NCBI gene IDs:
"Klp6: 2539700
Mcp1: 2542755"
Figure legend: " (C) Intensity of Tea2 at plus ends quantitated from multiple kymographs of the type in (B) for control (n=16), ∆klp6 (n=13) or ∆mcp1 (n=14) cells "
NCBI gene IDs:
"klp6: 2539700
mcp1: 2542755"
Figure legend: "(D) Kymographs showing fluorescently-tagged MTs co-imaged with fluorescently-tagged Klp5/Klp6 in control (top panels, repeated from 1C) or ∆tea2 cells (bottom panels). Dashed yellow line indicates the cell end"
NCBI gene IDs:
"tea2: 2539857"
Figure legend: "(b) Total RNA sequencing of cenRNAs in yeasts. Left; read counts per million tracks for CEN16 in wildtype (black, top) and DAD1E50D humanized (red, bottom) yeasts. Right; heatmap of cenRNAs transcripts per million counts across all chromosomes in wildtype and DAD1E50D humanized strains. Centromere VI is marked with an asterisk to indicate that the plasmid encoding the histone plasmid also encodes a CEN6 sequence, which reads likely emanant"
NCBI gene IDs:
"histone: 
DAD1: 851579"
Figure legend: "(c) RT-PCRs of the indicated RNAs. Lane 1 is genomic DNA, lane 2 is wildtype shuffle strain (yDT67), lane 3 is BY4742, lane 4 is DAD1E50D humanized, lane 5 is scc4D65Y humanized, lanes 6-9 are the same as 2-5, but without reverse transcriptase added, lane 10 is no DNA."
NCBI gene IDs:
"DAD1: 851579
scc4: 856890"
Figure legend: "(e) DASH mutants increase histone-humanization in the scc4D65Y background. Humanization rates of the scc4D65Y mutant with and without DAD1E50D or DAM1N80Y. Significance of the mean difference in 5-FOAR frequency was determined with the Mann-Whitney test. Each dot represents one biological replicate (n = 8), central band represents the median, box extends from the 25th to 75th percentiles, and whiskers represent the minimum and maximum."
NCBI gene IDs:
"DAD1: 851579
DAM1: 853010
scc4: 856890"
Figure legend: "(f) Ploidy analysis by flow cytometry in the scc4D65Y humanized mutants from panel d. Flow cytometry data from humanized lineages yHs5 and yHs7 are shown for aneuploid and euploid controls, respectively."
NCBI gene IDs:
"scc4: 856890"
Figure legend: "(a) Growth assays of wild type, ipl1-2 mutants, and ipl1-2 kinetochore double mutants at permissive (24ºC) and non-permissive temperature (32ºC). ﻿Ten-fold serial dilutions of ~1.0 OD600 yeast cultures were spotted onto YPD solid media and grown at the indicated temperatures for four and three days, respectively."
NCBI gene IDs:
"ipl1: 855892"
Figure legend: "(b) Flow cytometry analysis of wild type, ipl1-2 mutants, and ipl1-2 kinetochore double mutants at the permissive (24˚C) and non-permissive temperature (30ºC). Yeast cultures were grown to logarithmic phase at 24˚C and divided and grown at 24˚C and 30˚C for 6 hours before cells were collected and processed for DNA content analysis using Sytox Green stain."
NCBI gene IDs:
"ipl1: 855892"
Figure legend: "(c) Growth assays of wild type, mad3∆ mutants, and mad3∆ DAD1E50D double mutants on YPD with and without 30 µg/ml benomyl. Ten-fold serial dilutions of ~1.0 OD600 yeast cultures were spotted onto the indicated solid media and grown at 30˚C for three days."
NCBI gene IDs:
"DAD1: 851579
mad3: 853439"
Figure legend: "(b) Histone-humanization assay for various dam1N80X mutants. 5-FOA is used to counter-select the yeast histone plasmid, forcing growth with the human histone plasmid. Yeast were serially diluted from a starting culture of 1.0 OD600."
NCBI gene IDs:
"dam1: 853010
histone: 126961///8358///391769///440093///340096///8350///8968
histone: 851811///855976///852284///854150///851810///855701///852294///855700///852295///852283"
Figure legend: "(a) Sporulation efficiency after five days in sporulation medium at 25 ˚C. Example micrographs are shown to the left for both wildtype and DAD1E50D SK1 strains. Right, quantification of average sporulation efficiency from all micrographs. Two tailed unpaired t test of the mean difference in sporulation efficiency. Each dot represents the fraction of cells sporulated in a single field of view (technical replicates; n ≥15), central band represents the median, box extends from the 25th to 75th percentiles, and whiskers represent the minimum and maximum."
NCBI gene IDs:
"DAD1: 851579"
Figure legend: "(b) Spore viability assay. Individual tetrads were micromanipulated on a agar surface and spores were dissected and arrayed. Genotype was determined by PCR genotyping of the DAD1 locus. Quantification from all tetrad dissections performed, total are shown below. The one-tailed probability of observing 51% spore viability in DAD1E50D SK1 over a total of 139 tetrads is p &lt; 0.000001, assuming a spore viability of ~90% from wild type SK1."
NCBI gene IDs:
"DAD1: 851579"
Figure legend: "RT-qPCR assay on nuclear  RNA for assessing the uncleaved/total ratio of p53  and TATA-binding protein (TBP) pre-mRNAs in A549  cells (n=3) transfected for 48 hours with two different siRNA targeting PCF11  prior to exposure (UV+) or not (UV-) to  UV irradiation  (40 J/m2). \"n\" indicates the number of biological replicates for each experiment. All data are presented as the mean ± s.e.m. P values were calculated using two-sided unpaired t-test."
NCBI gene IDs:
"PCF11: 51585
TATA-binding protein: 6908
TBP: 6908
p53: 7157"
Figure legend: "RT-qPCR  analysis on RNA extracted from nucleoplasm  and chromatin  fractions. The ratio of uncleaved pre-mRNA (nucleoplasm/chromatin) was calculated to quantify the level of unprocessed p53, TBP, WDR13  and GAPDH  pre-mRNA (n=3 biological replicates) released in the nucleoplasm  compared to the chromatin-bound unprocessed pre-mRNA. WDR13  and GAPDH  were included as controls as they have been previously reported to be processed post- and co-transcriptionally, respectively. Data are presented as the mean ± s.e.m."
NCBI gene IDs:
"GAPDH: 2597
TBP: 6908
p53: 7157
WDR13: 64743"
Figure legend: "RT-PCR  analysis of p53  3' flanking region using the same primers employed in the data panel above (n=3). Lane 1 is a control PCR  amplification of cDNA derived from the reverse transcription  of a control mRNA using  oligo (dT). Lanes 2-7 are PCR  amplification of reverse transcribed p53 chromatin-associated pre-mRNA using oligo oligo (dT)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "RT-qPCR  assay on nuclear  RNA for assessing the uncleaved/total ratio of p53  pre-mRNA in wild type (WT) and CoTC  deleted (ΔCoTC) A549  cells (n=3) treated with or without UV irradiation (40 J/m2). \"n\" indicates the number of biological replicates for each experiment. All data are presented as the mean ± s.e.m. P values were calculated using two-sided unpaired t-test."
NCBI gene IDs:
"p53: 7157"
Figure legend: "RT-qPCR measuring relative p53  and p21  mRNA levels in wild type (WT) and CoTC-deleted (ΔCoTC) cells (n=3) in response to UV  treatment (40 J/m2). The expression was normalized to HPRT. \"n\" indicates the number of biological replicates for each experiment. All data are presented as the mean ± s.e.m. P values were calculated using two-sided unpaired t-test."
NCBI gene IDs:
"p21: 1026
HPRT: 3251
p53: 7157"
Figure legend: "Real-time qPCR analysis of fetal organoids cultured for one month showing decrease in relative expression of indicated neonatal markers A Ass1, B Blimp-1, C FcRn and D Lct and increase of the adult markers E Sis, F Treh and G Arg2 (n=3 individual wells from single organoid culture (see material and methods); experiment was repeated in four to eight independent organoid cultures with similar results)."
NCBI gene IDs:
"Arg2: 11847
Ass1: 11898
FcRn: 14132
Lct: 226413
Blimp-1: 12142
Sis: 69983
Treh: 58866"
Figure legend: "Relative expression detected by Real-time qPCR in fetal organoids ( ) and adult organoids ( ). Neonatal markers A Ass1, B Blimp-1, C FcRn and D Lct in fetal organoids cultured for one month compare to levels adult organoids. Mature markers E Sis, F Treh and G Arg2 increase in fetal organoids to adult levels (n=3 individual wells from single organoid culture (see material and methods); experiment was repeated in four independent organoid cultures with similar results)."
NCBI gene IDs:
"Arg2: 11847
Ass1: 11898
FcRn: 14132
Lct: 226413
Blimp-1: 12142
Sis: 69983
Treh: 58866"
Figure legend: "Maturation is accelerated in dexamethasone treated fetal organoids ( ) compared to control ( ), as revealed by real-time qPCR. Decrease in relative expression of neonatal marker A Blimp-1 and increase in expression of adult marker B Sis occur earlier in dexamethasone treated organoids"
NCBI gene IDs:
"Blimp-1: 12142
Sis: 69983"
Figure legend: "Maturation is accelerated in dexamethasone treated fetal organoids ( ) compared to control ( ), as revealed by real-time qPCR. expression of adult marker E Treh and F Arg2 remains unchanged (n=3 individual wells from single organoid culture"
NCBI gene IDs:
"Arg2: 11847
Treh: 58866"
Figure legend: "(F) Representative ET reconstructions of mitochondria from a WT cell (left), a Mic10-KO cell (center) and a Mic60-KO cell (right). The OM is displayed in clear grey, the side of the IM that faces the matrix is shown in dark blue. The IM side that faces the IMS is shown in light blue."
NCBI gene IDs:
"Mic60: 10989
Mic10: 440574"
Figure legend: "(A Nanoscopy of mitochondria of WT and Mic10-KO cells, as indicated. (A) Mitochondria were immunolabeled for Mic60 and ATPB and visualized by 2D STED nanoscopy."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(C) 3D MINFLUX nanoscopy. Mic10-KO cells were immunolabeled for Mic60 using a directly labeled antibody. Colors encode depth information."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(D) 3D SIM of living WT and Mic10-KO cells. Cells were labeled with Mitotracker Green. Images show maximum intensity projections."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(E) FIB-SEM of Mic10-KO cells. Shown is a representative reconstructed mitochondrion. Dashed box: Orthoslice view on the smaller mitochondrion. The OM and IBM are displayed together in clear grey, the CM in is shown in blue."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(F) 2D STED nanoscopy of mitochondria. Mic19-KO cells were transfected with a plasmid for Mic10-FLAG expression under the control of a tetracycline-inducible promoter. Left panel: Mitochondria from noninduced cell. Right: Mitochondria from induced cell expressing Mic10-FLAG."
NCBI gene IDs:
"FLAG: 
Mic19: 54927
Mic10: 440574"
Figure legend: "(A) Cells were immunolabeled for Mic60 and DNA and visualized by confocal fluorescence microscopy before and after induction of Mic60 expression for 48 h."
NCBI gene IDs:
"Mic60: 10989"
Figure legend: "(B) Protein levels of MICOS proteins after Mic60 induction. Cell lysates were analyzed by western blotting at the indicated time points."
NCBI gene IDs:
"Mic60: 10989"
Figure legend: "(C) Recovery of mitochondrial networks upon Mic60 re-expression. Cells were induced with doxycycline hyclate for 72 h, immunolabeled for TOM20 and visualized by confocal microscopy."
NCBI gene IDs:
"Mic60: 10989"
Figure legend: "(D-G) TEM of Mic60-TO cells before and after induction. (D) TEM recordings of Mic60-TO cells at the indicated time points. (E) Quantification of the cristae morphology. (F) CJ frequency. The number of CJs on TEM recordings was normalized to the length of the OM. At least 90 mitochondrial sections were analyzed for each sample. (G) Quantification of the cristae morphology of Mic60-TO cells. Only mitochondria exhibiting at least one CJ were analyzed. Data information: n: Number of mitochondrial sections. Scale bars: 20 µm (A), 10 µm (C), 500 nm (D)."
NCBI gene IDs:
"Mic60: 10989"
Figure legend: "(A,B) Live-cell nanoscopy of Mic10-TO cells. Cells expressing COX8A-SNAP were labeled with SNAP-cell SiR and recorded with STED nanoscopy. (A) Cristae architecture in noninduced cells. (B) Cristae architecture after Mic10 re-expression (24 h induction with doxycycline). STED image data were deconvolved."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(C) STED nanoscopy of fixed Mic10-TO cells. Cells were induced for 24 h and immunolabeled for Mic60 and Mic10-FLAG. Upper row: Cell with weak Mic10 expression level. Lower row: Cell with strong Mic10 expression level."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(D-G) TEM of Mic10-TO cells. (D) Cells induced for the indicated period of time were recorded with TEM. Arrow indicates an intermediately shaped crista. (E) Quantification of the cristae morphology. n: Number of mitochondrial sections. (F) CJ frequency in Mic10-TO cells. Number of CJs was normalized to the length of the OM. The same sections were analyzed as in (E). (G) Diameter of CJs estimated on TEM recordings. Data information: n: Number of analyzed mitochondrial sections (E) or CJs (G).. "
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(A) FIB-SEM of Mic10-TO cells. Cells were induced for 0 h, 16 h and 24 h. The representative reconstructions show mitochondria from noninduced, rescued and intermediate cells, as indicated. The OM is shown together with the IBM in clear grey, the CM in blue."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "(B) ET of Mic10-TO cells. Mic10 expression was induced for 0 h and 16 h. Shown are reconstructions of representative noninduced and almost completely rescued mitochondria. The OM is displayed in clear grey, the side of the IM that faces the matrix is shown in dark blue. The IM side that faces the IMS is shown in light blue."
NCBI gene IDs:
"Mic10: 440574"
Figure legend: "Knockdown (KD) of OPA1 in HeLa cells. WT and Mic60-KO cells were transfected with a scrambled control (Ctrl.) or siRNA pools against OPA1 (OPA1-KD) for 5 d. (A) Cell lysates were analyzed by western blotting. "
NCBI gene IDs:
"Mic60: 10989
OPA1: 4976"
Figure legend: "(B) TEM recordings from OPA1 depleted WT and Mic60-KO cells. Arrows point to a septum."
NCBI gene IDs:
"Mic60: 10989
OPA1: 4976"
Figure legend: "(C,D) Quantification of the cristae morphology of OPA1 deficient WT (C) and Mic60-KO (D) cells. The number of CJs was normalized to the length of the OM (C). The number of septated mitochondria in Mic60-KO cells was estimated (D)."
NCBI gene IDs:
"Mic60: 10989
OPA1: 4976"
Figure legend: "(E,F) ET of mitochondria from OPA1-KD cells. WT cells were transfected with a scrambled control (E) or siRNA pools against OPA1 (F) for 48 h. Reconstructions of representative mitochondria are displayed. The OM is displayed in clear grey, the side of the IM that faces the matrix is shown in dark blue. The IM side that faces the IMS is shown in light blue."
NCBI gene IDs:
"OPA1: 4976"
Figure legend: "WT and Mic10-KO cells were transfected with a scrambled control (Ctrl.) or siRNA pools against OPA1 (OPA1-KD) for 72 h. (A,B) STED nanoscopy of OPA1 depleted WT (A) and Mic10-KO (B) cells. Cells were immunolabeled for Mic60 and ATPB."
NCBI gene IDs:
"Mic10: 440574
OPA1: 4976"
Figure legend: "(C) TEM of OPA1 depleted Mic10-KO cells. Left: Representative TEM recordings showing CJs marked by arrows in a control cell and a similar view for an OPA1-KD cell. The CMs are colored in green and the OM and IBM together in magenta. Right: Quantification of mitochondria containing CJs or septa junctions. Sections that contained CJ-like structures were analyzed. Of these, the number of sections exhibiting septa or cristae structures (other) was quantified."
NCBI gene IDs:
"Mic10: 440574
OPA1: 4976"
Figure legend: "(D Depletion of ATP5ME in HeLa cells. Cells were transfected with a scrambled control or siRNA pools against ATP5ME for 4 d. TEM recording (left) and quantification of the number of cristae per mitochondrial section (right)."
NCBI gene IDs:
"ATP5ME: 521"
Figure legend: "(E) Cells depleted of ATP5ME were immunolabeled for Mic60 and recorded with STED. The arrows point to crossing points of the opposite Mic60 distribution bands."
NCBI gene IDs:
"ATP5ME: 521"