EMBO/SourceData huggingface dataset:
Figure legend: "G, Immunofluorescence  images of 12 μm skin  section of PD17 induced PD25 CT and Alk1LymphKO mice stained  for Ki67  (red), Itga6  (green) and Dapi  (blue) showing representative hair follicle. Hair follicles were outlined with yellow dotted lines. Note Ki67+ cells in the hair germ (HG) area indicative of HFSC  activation. Telogen hair follicle  is defined as no Ki67  in the hair germ. Anagen I is defined as ≤7 Ki67+ cells in the unenlarged hair germ. Anagen II  is defined as &gt;7 Ki67+ cells in the hair germ or enlarged hair germ  with changed shape that starts to enclose the DP. Experiments were repeated at least 2 times with at least 3 skin  sections/each mouse  analyzed with N = 6/4 mice/group. See Table 1 for quantification detail."
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "B, Immunofluorescence  image of 12 μm skin  section from CT and  Alk1EndoKO mice  used for RNA-seq stained  for Ki67  (red) and Dapi  (blue) show that HFSCs  are in quiescence."
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "C, Images of 20 μm skin  section of PD20 and PD25 BMP4-LacZ; Alk1f/f; Cdh5-CreERT2 negative, injected with tamoxifen TM  at PD17 (labelled as CT), and PD20/PD25 Cdh5CreERT2; Alk1f/f; BMP4-LacZ mice  (labelled as Alk1EndoKO) TM  injected at PD17, all stained  for CD31  (IHC  method, brown) and X-gal  (blue). Hair follicles  (HF) were outlined with black dotted lines. Arrows point to X-Gal+ vascular  structure in HPuHG  area."
NCBI gene IDs:
"LacZ: 
Alk1: 11482
BMP4: 12159
Cdh5: 12562
Cre: 2777477
ERT2: 2099"
Figure legend: "K, Quantification of hair follicle percentage of PD17-induced PD25 CT,  Alk1EndoKO, Alk1 BMP4dKO, and BMP4EndoKO at telogen, anagen I, and  anagen II stages. Telogen hair follicle is defined as no Ki67  in the hair germ. Anagen I is defined as ≤7 Ki67+ cells in the unenlarged  hair germ.  Anagen II is defined as &gt;7 Ki67+ cells in the hair germ or enlarged hair germ with changed shape that starts to enclose the DP. P-value was generated from Chi-square test. See Table 1 for quantification detail."
NCBI gene IDs:
"Alk1: 11482
BMP4: 12159"
Figure legend: "B.  Western blot  analysis of the   dose-dependent  expression of ZBP1  in HT29  / TO-ZBP1WT and HT29  / TO-ZBP1Zα1α2mut cells treated with indicated concentrations of Dox  for 24 h. Blots  are representative of three biological repeats."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "L. Transwell migration of primary neutrophils  towards conditioned media from HT29  / TO-ZBP1WT cells treated with DMSO  or 500 ng/mL Dox  for 24 hours, or HT29  chemotaxis buffer (control buffer) containing equal volume and amount of DMSO  or Dox  as the conditioned media. Data is presented as individual values with grand mean of the migrated percentage induced by control buffer containing DMSO  or Dox  (n = 10 biological replicates), or conditioned media from DMSO-treated cells (n = 11 biological replicates) or Dox-treated cells (n = 12 biological replicates), where the number of biological replicates is defined by the total number of independent cell cultures tested on primary neutrophils  from four donors. An unpaired t-test was used to test for statistical differences between indicated conditions. **** p &lt; 0.0001."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "M. Transwell migration of primary neutrophils towards conditioned media from HT29  / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  in combination with DMSO  or 20 µM zVAD  + 10 μM Nec1s  for 24 h. Data is presented as individual values with mean and S.E.M. (n = 3 biological replicates of conditioned media). One-way ANOVA and Sidak's multiple comparisons test were used to test for statistical differences between indicated conditions. ns = not significant (p = 0.4129); *** p = 0.0004."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "C. Transwell migration of primary neutrophils towards conditioned media from HT29  / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  in combination with indicated inhibitors for 24 h. Data is presented as individual values with grand mean (n = 3 biological replicates of conditioned media from GSK'872  and NSA-treated wells, n = 6 biological replicates of conditioned media from other conditions). Two-way ANOVA and Sidak's multiple comparisons test were used to test for statistical differences between indicated conditions. **** p &lt; 0.0001; ns = not significant (p &gt; 0.96)."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "D. CXCL8  concentration in the culture media of HT29  / TO-ZBP1WT cells transfected with siRNA targeting mismatch sequence (MM) or RIPK3  and treated with Dox  at indicated concentrations for 24 h. Data is presented as mean with S.E.M. (n = 4 biological replicates). Unpaired t-tests were used to test for statistical differences between indicated conditions. ** p = 0.0079. Cell lysates from one biological replicate were analyzed by Western blotting."
NCBI gene IDs:
"RIPK3: 56532
ZBP1: 81030"
Figure legend: "E. Cytokine concentration  in the culture media of HT29  / TO-ZBP1WT or HT29  / RIPK1-KO clone aA3 / TO-ZBP1WT cells treated with 0, 50 or 500 ng/mL Dox. data is plotted as mean with S.E.M. (n = 6 biological replicates). Brown-Forsythe and Welch ANOVA tests and Dunnet's T3 multiple comparisons test were used to test for statistical differences between indicated conditions. Cell lysates were analyzed by Western blotting for ZBP1  levels. Blots  are representative of six biological replicates. ns = not significant (p &gt; 0.2); *** p = 0.0003 for CXCL8, p = 0.0008 for CXCL1; **** p &lt; 0.0001."
NCBI gene IDs:
"RIPK1: 19766
ZBP1: 81030"
Figure legend: "G, NF-κB  activity in HEK293FT  cells with and without stable expression of full-length (FL) RIPK3-2xFV or RIPK3∆C-2xFV (∆C) transfected with dual luciferase  reporters and ZBP1  or empty vector (EV) as indicated. Reporter activities were measured 24 h after transfection. Data is presented as mean with S.E.M (n = 3 biological replicates). One-way ANOVA and Sidak's multiple comparisons test were used to test for statistical differences between indicated conditions. ns = not significant (p &gt; 0.05); **** p&lt;0.0001. (G) Cell lysates were analyzed by Western blotting. Blots  are representative of three biological replicates. Asterisk indicates background signals of the antibody."
NCBI gene IDs:
"luciferase: 
RIPK3: 11035
ZBP1: 81030"
Figure legend: "A. Western blot  analysis of anti-FLAG  (ZBP1) immunoprecipitation  from HT29  / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  for 16 h. Blots  are representative of three biological replicates. Immunoprecipitated  material was treated or not with USP21  (1 μM) for deubiquitination. Input loaded was 5% for ZBP1  and 1% for co-immunoprecipitants. Asterisk indicates antibody heavy chain signal in IP  samples."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "B. Enrichment of Ub-conjugates by GST-1xUBA   for analysis of ubiquitination  status of RIPK1, ZBP1  and RIPK3  in HT29  / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  for 16h. Blots  are representative of at least three biological replicates. After enrichment, samples were treated or not with USP21  (1 μM) for deubiquitination. Asterisk indicates unspecific bands. Arrows indicate RIPK3  signal."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "C. Enrichment of Ub-conjugates for analysis of the ubiquitination  status of RIPK1  and ZBP1  using GST-1xUBA or linkage-specific SUBs  in HT29  / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  for 16 h."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "D. Enrichment of Ub-conjugates by GST-1xUBA for analysis of ubiquitination  status of RIPK1  and ZBP1  in HT29, HT29  / TO-ZBP1WT and HT29  / RIPK1-KO aA3 / TO-ZBP1WT cells treated with 500 ng/mL Dox  for 16 h. Blots  are representative of four biological replicates. Asterisk indicates antibody background signal."
NCBI gene IDs:
"RIPK1: 19766
ZBP1: 81030"
Figure legend: "E.  NF-κB activity in HEK293FT  / RIPK3-2xFV cells transfected with dual luciferase  reporters, ZBP1  or EV, and GFP  (Ctrl) or GFP-tagged SUBs  as indicated. Luciferase reporter activities  were measured 24 h after transfection and normalized to GFP+EV-transfected condition. Data is plotted as mean with S.E.M. (n = 4 biological replicates). One-way ANOVA and Sidak's multiple comparisons tests were used to test for statistical differences between the indicated condition and ZBP1/GFP-transfected condition. **** p &lt; 0.0001. Cell lysates were analyzed by Western blotting  to determine expression of ZBP1, GFP  or GFP-tagged SUBs  and Actin. Blots  are representative of three biological replicates."
NCBI gene IDs:
"GFP: 
luciferase: 
RIPK3: 11035
ZBP1: 81030"
Figure legend: "F, NF-κB activity in HEK293FT  / RIPK3-2xFV cells transfected with dual luciferase  reporters, ZBP1  or EV, and variants of OTULIN.  Luciferase reporter activities  were measured 24 h after transfection and normalized to ZBP1/EV-transfected cells. Data is plotted as mean with S.E.M. Multiple Welch t-tests were used to test for statistical differences between the indicated condition and the ZBP1/EV-transfected condition. (F) n = 4 biological replicates. ** p = 0.0012, ns = not significant (p = 0.2342). Cell lysates were analyzed by Western blotting to determine expression of ZBP1, or OTULIN, and Actin. Blots  are representative of (F) four biological replicates."
NCBI gene IDs:
"luciferase: 
OTULIN: 90268
RIPK3: 11035
ZBP1: 81030"
Figure legend: "G. NF-κB activity in HEK293FT  / RIPK3-2xFV cells transfected with dual luciferase  reporters, ZBP1  or EV, and variants of CYLD Luciferase reporter activities were measured 24 h after transfection and normalized to ZBP1/EV-transfected cells. Data is plotted as mean with S.E.M. Multiple Welch t-tests were used to test for statistical differences between the indicated condition and the ZBP1/EV-transfected condition. (G) n = 3 biological replicates. * p = 0.0137; ns = not significant (p = 0.8374). Cell lysates were analyzed by  Western blotting to determine expression of ZBP1, CYLD  and Actin. Blots  are representative of (G) three biological replicates."
NCBI gene IDs:
"luciferase: 
CYLD: 1540
RIPK3: 11035
ZBP1: 81030"
Figure legend: "A. NF-κB  activity in HEK293FT  / RIPK3-2xFV cells transfected with siRNA targeting HOIP  (siHOIP) or a mismatch sequence (siMM), and 48 h later transfected with dual luciferase  reporters and ZBP1  or GFP  (control). Luciferase reporter activities  were measured 24 h after transfection and normalized to the siMM+ZBP1  samples. Data is plotted as mean with S.E.M. (n = 4 biological replicates). A Welch's t-test was used to test for the statistical difference between indicated conditions. **** p &lt; 0.0001. HOIP   knockdown and ZBP1  expression levels were analyzed by Western blotting. Blots  are representative of three biological replicates."
NCBI gene IDs:
"GFP: 
luciferase: 
RIPK3: 11035
HOIP: 55072
ZBP1: 81030"
Figure legend: "D-F. Western blot  analysis of anti-FLAG  (ZBP1) immunoprecipitation  from HT29  or HT29  / TO-ZBP1WT or HT29  / RIPK1-KO clone aA3 / TO-ZBP1WT cells treated with 0 or 500 ng/mL Dox  for 16 h. Asterisk indicates antibody heavy chain signal in IP  samples. Blots  are representative of at least (D) two and (E-F) three biological replicates."
NCBI gene IDs:
"RIPK1: 19766
ZBP1: 81030"
Figure legend: "B. Western blot analysis of HCT116  / RIPK3-2xFV cells (FL and ∆C) treated with 100 nM dimerizer. Blots  are representative of two biological replicates."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "D. CXCL8  concentration in the culture media of HCT116  / RIPK3-2xFV cells (WT and RHIMmut) treated with 100 nM dimerizer  for 24 h. In RIPK3-RHIMmut three key residues, VQV, of the RHIM region was changed to AAAA. Data is plotted as mean with S.E.M. (n = 5 biological replicates). One-way ANOVA and Sidak's multiple comparisons tests were used to test for statistical differences between indicated time points. ** p = 0.0011; ns = not significant (p = 0.9301). Inset: Western blot analysis to determine RIPK3  expression levels in one biological replicate. Line indicates that image was cut and spliced to remove non-relevant lanes from the scanned blot."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "H. Relative mRNA levels of CXCL8 in HCT116  / RIPK3-2xFV cells pretreated with 100 nM CpA  for 0.5 h before treatment with 0 or 100 nM dimerizer  for 3 h. Data is presented as mean with S.E.M. (n = 3 biological replicates). One-way ANOVA and Sidak's multiple comparisons tests were used to test for statistical differences between indicated conditions and DMSO+dimerizer-treated condition. **** p &lt; 0.0001. (H) Cell lysates were analyzed by Western blotting for cIAP1  levels. Blots  are representative of two biological replicates."
NCBI gene IDs:
"CXCL8: 3576
RIPK3: 11035"
Figure legend: "J. Relative CXCL8  mRNA levels in WT or HOIP-knockout HCT116  / RIPK3-2xFV cells treated with 0 or 100 nM dimerizer  for 3 h. Data is plotted as mean with S.E.M. (n = 3 biological replicates). A Welch's t-test was used to test for the statistical difference as indicated. *** p = 0.0010. Cell lysates were loaded for Western blot  analysis."
NCBI gene IDs:
"CXCL8: 3576
RIPK3: 11035
HOIP: 55072"
Figure legend: "K. Relative CXCL8  mRNA levels in HCT116  / Tet-On-GFP-K63-SUB  / RIPK3-2xFV, HCT116  / Tet-On-GFP-M1-SUB  / RIPK3-2xFV and HCT116  / Tet-On-GFP  / RIPK3-2xFV cells treated with 0 or 100 ng/mL Dox  for 48 h before stimulated with 0 or 100 nM dimerizer  for 3 h. Data is presented as mean with S.E.M. (n = 4 biological replicates). Brown-Forsythe and Welch ANOVA tests and Dunnet's T3 multiple comparisons test were used to test for statistical differences between indicated conditions. ns = not significant (p = 0.9997); ** p = 0.0030; * p = 0.0366. Cells were analyzed by Western blotting  for the inducible-expression levels of GFP  (control) and GFP-SUBs. Blots are representative of three biological replicates."
NCBI gene IDs:
"GFP: 
CXCL8: 3576
RIPK3: 11035"
Figure legend: "M. Relative CXCL8  mRNA levels in HCT116  / RIPK3-2xFV cells stably knocked down against mismatch (shMM) or two different sites of  caspase-8 (shCASP8-A and shCASP8-B), pretreated with DMSO, 10 µM Nec1s, 10 µM GSK'872 or 1 µM NSA  for 1h followed by treatment with 0 or 100 nM dimerizer  for 3h. Data is plotted as mean with S.E.M. (n = 3 biological replicates). Two-way ANOVA and Tukey's multiple comparison tests were used to test for statistical differences between indicated conditions. *** p = 0.0001; ** p = 0.0035. Cell lysates from one biological replicate were analyzed by Western blotting to determine caspase-8 levels."
NCBI gene IDs:
"CASP8: 841
caspase-8: 841
CXCL8: 3576
RIPK3: 11035"
Figure legend: "A. Western blot analysis of ZBP1, RIPK1, RIPK3, MLKL  levels in Calu-3  cell lines with stable expression of shRNAs targeting ZBP1  or a mismatch sequence (shMM), untreated (NT) or treated with 10 ng/mL IFNβ  for 48 h. Blots  are representative of two biological replicates."
NCBI gene IDs:
"ZBP1: 81030"
Figure legend: "somatic hypermutation (SHM) count (E) in IGHV3-21 and IGHV3-33 genes from MBCs"
NCBI gene IDs:
"IGHV3-21: 28444
IGHV3-33: 28434"
Figure legend: "(C) VH SHM load of VH3-21 and non-VH3-21 mAbs. mAbs with unmutated VH are highlighted in green."
NCBI gene IDs:
"non-VH3-21: 
VH3-21: 28444"
Figure legend: "(D) Amino acid (aa) VH replacement (red bars) and silent (black bars) SHM in VH3-21 mAbs (n=113). FWR, framework region; CDR, complementarity-determining region."
NCBI gene IDs:
"VH3-21: 28444"
Figure legend: "(D) C-CSP reactivity of the α-TSR-specific mAb 1710 in a blocking ELISA with C-CSP-specific VH3-21 (n=21; upper panel) or non-VH3-21 (n=17; lower panel) mAbs with the indicated SPR affinities. mAb 1710 binding without blocking (blue) and after self-blocking (gold) is shown for comparison."
NCBI gene IDs:
"non-VH3-21: 
VH3-21: 28444"
Figure legend: "(B) The Western blot analysis of GFP, Spd2 and αTub (loading control) in the brain extracts from wild type (Oregon R, OR), Spd2WT-OE and Spd2DK-OE third instar larva. Representative immune blot images are shown on the left. The signal intensities of Spd2 bands were quantified and normalised against αTub values. The values relative to control of each biological replicate (n = 2) and their means are shown as dots and bars, respectively, in a scatted dot plot on the right. The numbers above the bars indicate means ± Range. GFP-Spd2DK proteins in Spd2DK-OE larval brain extracts showed approximately 2.04-fold higher accumulation than GFP-Spd2WT proteins in Spd2WT-OE brain extracts. (C) The Western blot analysis of Spd2, CycB and αTub in OR, fzrRNAi, Spd2WT-RES and Spd2DK-RES brain extracts. The signal intensities of Spd2 bands were quantified and normalised against αTub values. The values relative to control and their means are presented in a scatted dot plot on the right (biological replicate n = 2). Means ± Range are shown above the bars. RFP-Spd2DK proteins in Spd2DK-RES larval brain extracts showed approximately 2.08-fold higher accumulation than RFP-Spd2WT proteins in Spd2WT-RES brain extracts."
NCBI gene IDs:
"GFP: 
RES: 
RFP: 
fzr: 45922
Spd2: 39850"
Figure legend: "(D) Larval brains of OR, Spd2WT-RES, fzrRNAi and Spd2WT-OE flies were stained by an anti-Spd2 antibody. Representative images of NBs are shown on the top. Scale bar: 10 µm. The local signal intensities of Spd2 immunofluorescence at the centrosomes and in the cytoplasm of individual NBs in each brain were measured, and the normalised Spd2 signal intensities in individual NBs were presented as scattered dot plots (see Materials and Methods for detail). The lines indicate means ± SD. The numbers of NBs (n) and the numbers of brains analysed (N) are control: n= 29 (N = 6), Spd2WT-RES: n = 30 (N = 4), fzrRNA: n = 30 (N = 6), and Spd2WT-OE: n = 27 (N = 10). P values were calculated using non-parametric two-tailed Mann-Whitney tests. Based on these results, the relative centrosomal Spd2 protein levels in NBs can be estimated as wildtype (or control) : Spd2WT-RES: fzrRNAi: Spd2DK-RES: Spd2WT-OE: Spd2DK-OE ­= 1: 1.00~1.18: 1.06~1.29: 2.08~2.45 (= 1.00~1.18 x 2.08): 1.93~3.62: 3.94 ~ 7.38 (= 1.93~3.62 x 2.04"
NCBI gene IDs:
"RES: 
fzr: 45922
Spd2: 39850"
Figure legend: "(C) In time lapse movies, NBs that showed an active centrosome that was attached to the cortex (&quot;Attached&quot;) and NBs with both two centrosomes being inactivated and detached from the cortex (&quot;Detached&quot;) in interphase were counted in third instar larval brains of the indicated fly lines and their proportions are presented in bar graphs. The numbers of NBs analysed from at least four brains (n) are Control: 29, fzrRNAi: 80, Spd2WT-RES: 45, Spd2DK-RES: 52, Spd2WT-OE: 43, Spd2DK-OE: 40, Spd2WT-OE + fzrRNAi: 72. p-values are fzrRNAi: 0.0069, Spd2WT-RES: 0.2479, Spd2DK-RES: 0.0116, Spd2WT-OE: &lt;0.0001, Spd2DK-OE: &lt;0.0001, Spd2WT-OE + fzrRNAi: 0.0008 (Pearson's chi-squared tests were performed between control and each condition)."
NCBI gene IDs:
"RES: 
fzr: 45922
Spd2: 39850"
Figure legend: "(E) The signal intensities of the indicated centriolar and PCM proteins at two centrosomes were quantified in individual interphase NBs in Spd2WT-RES and Spd2DK-RES larval brains, and their asymmetric distributions of their centrosomal signals between the two centrosomes are presented as Asymmetric Indexes (see Materials and Method) in a scatted dot plot. Red bars represent means ± SD. The numbers of NBs analysed from at least 3 different brains of Spd2WT-RES and Spd2DK-RES (n) were 40 and 40 for Asl, 40 and 41 for RFP-Spd2, 42 and 37 for Cnn, 40 and 41 for γTub, and 40 and 40 for AurA."
NCBI gene IDs:
"RES: 
Spd2: 39850"
Figure legend: "(D) Division axis deviations were separately analysed in NBs with apically attached interphase centrosomes (&quot;attached&quot;) and those with centrosomes being detached from the cortex (&quot;detached&quot;) for each line, using the same dataset as in Fig. 3C. The same control data as in Fig. 3C is shown for reference. fzrRNA detached (mean ± SD: 34.62° ± 23.39), Spd2DK-OE detached (64.01° ± 33.93), and Spd2WT-OE+fzrRNAi attached (39.55° ± 27.08) and detached (59.08° ± 34.88) brains showed statistically significant differences from control, while NBs with cortically attached centrosomes in any of the lines did not show significant differences. n: the number of NB divisions analysed from at least four brains for each line. p-values were calculated using Mann-Whitney U tests comparing with control and are shown above the dots."
NCBI gene IDs:
"fzr: 45922
Spd2: 39850"
Figure legend: "(B) Selected images from time-lapse microscopy of NBs in control (wor&gt;mCh-Tub, pUbq-GFP-Fzr, top panels) and Spd2DK-OE (lower panels) larval brains (Movies EV1, EV10). In the control NB, the daughter centrosomes (blue arrowheads) first matured (-5.0 min) and was segregated into the daughter NB (12.0 min), while the mother centrosome (yellow arrowheads) matured a little later (0.0 min) and segregated into the GMC. In contrast, in the Spd2DK-OE NB, both centrosomes lost microtubule nucleation activity in interphase (red arrowheads). The centrosome that matured earlier (blue arrowheads, -6.0 min) was incorrectly segregated into the GMC (12.0 min) while the centrosome that matured later (yellow arrowheads, 0.0 min) was segregated into the NB (12.0 min). Dotted white lines outline the dividing NBs and the newly formed GMC, which are also marked by asterisks."
NCBI gene IDs:
"GFP: 
mCh: 
Ubq: 
Fzr: 45922
Spd2: 39850
Tub: 7846
wor: 34906"
Figure legend: "(C, D) Representative images of interphase NBs in fixed wor&gt;lacZ (control, top panels) and HA-Spd2WT-OE larval brains expressing Polo-GFP from the native promoter and stained for DAPI and Spd2 (C). Asymmetric indexes of Polo-GFP in interphase NBs wor&gt;lacZ (control, top panels) and HA-Spd2WT-OE larval brains (D). 38 and 39 NBs (n) from at least three brains were analysed in each line. The asymmetric distribution of Polo-GFP at interphase centrosomes was unaffected by HA-Spd2WT overexpression."
NCBI gene IDs:
"HA: 
lacZ: 945006
Spd2: 39850
wor: 34906"
Figure legend: "(E) Representative images of interphase NBs in fixed mCh-Spd2WT-RES (top panels) and mCH-Spd2DK-RES larval brains and stained for DAPI, γTub and Cnn. Both multichannel and single-channel images of mCh-Spd2, γTub and Cnn are shown. Dotted yellow squares highlight centrosomes and their magnified images are shown in insets. Similar to Spd2DK-RES NBs, in mCh-Spd2CONS-RES NBs, Spd2 and γTub were more symmetrically accumulated at the two centrosomes than in mCh-Spd2WT-RES NBs, but Cnn was still asymmetrically distributed."
NCBI gene IDs:
"mCh: 
mCH: 
RES: 
Spd2: 39850"
Figure legend: "(G, H) FRAP analyses of the centrosomal Spd2 fluorescent signals in Spd2WT-RES and Spd2DK-OE NBs. (G) Representative images from time-lapse movies (Movies EV13, EV14) of Spd2 fluorescent signals in Spd2WT-RES NBs (top panels) and Spd2DK-OE NBs (lower panels) upon photobleaching. (H) The recoveries of the fluorescent intensities of centrosomal Spd2 signals after photobleaching were measured in Spd2WT-RES (n = 7) and Spd2DK-OE NBs (n = 5). Means ± SEM of the normalised fluorescent intensities are shown in a line graph. Centrosomal Spd2 signals recovered more fully after photobleaching in Spd2DK-OE NBs than in Spd2WT-RES NBs."
NCBI gene IDs:
"RES: 
Spd2: 39850"
Figure legend: "(B) Kaplan-Meier survival curves for colon cancer patients with or without dysfunction of iron homeostasis in the GSE dataset (n = 590 samples, P = 0.016, Log-rank (Mantel-Cox) test). \"Normal\" represents the patients with unaltered expression of iron homoeostasis-related genes, including LTF, CP, FTH1, SLC30A1 and TFRC; \"Dysfunctional iron homoeostasis\" represents the patients with altered expression of iron homoeostasis-related genes."
NCBI gene IDs:
"CP: 1356
FTH1: 2495
LTF: 4057
SLC30A1: 7779
TFRC: 7037"
Figure legend: " (E) Mass spectrum analysis of IREB2-associated proteins. Mock or IREB2-FLAG was transfected into HEK293T cells and FLAG-tagged proteins were enriched by anti-FLAG M2 beads and incubated with lysates from normal mice colon tissues. Five matched peptides corresponding to OTUD1 were shown on the right panel. "
NCBI gene IDs:
"FLAG: 
IREB2: 3658"