EMBO/SourceData huggingface dataset:
Figure legend: "F Comparison of TAZ gene expression, normalized to S12, in heart, liver, and kidney, analyzed in triplicates by quantitative PCR. n= 5 per genotype.G TAZ gene expression in percent. Wild-type is set to 100%."
NCBI gene IDs:
"TAZ: 66826"
Figure legend: "D Respiratory chain complexes in cardiac mitochondria were separated on BN-PAGE and stained for activity of complex I, II, IV and F1FO- ATPase. sh, shTAZ; Q, ubiquinone."
NCBI gene IDs:
"TAZ: 66826"
Figure legend: "C Steady state protein levels analyzed by SDS gel separation of indicated amounts of mitochondrial protein and subsequent Western- blotting with antibodies against SDHA and VDAC3 or visualizing of Flavin fluorescence after excitation with 488 nm. Lower panel: quantification of signal ratio SDHA/VDAC3 in shTAZ and control (set to 100%)."
NCBI gene IDs:
"TAZ: 66826"
Figure legend: "D Analysis of gene expression in cardiac tissue analyzed in (C) by qPCR using primers against indicated mRNA. Data of one representative measurement, normalized to b-actin with WT set to 100% are shown. Identical results were obtained in further experiments with two different pairs of animals."
NCBI gene IDs:
"b-actin: "
Figure legend: "B RT-PCR showing the expression of indicated genes in undifferentiated iPSCs and differentiated iPSC-CMs of BTHS patient TAZ10 and a healthy control."
NCBI gene IDs:
"TAZ: 6901"
Figure legend: "D Quantitation of sarcomere organization. Left panel: Immunostaining of cTNT, MLC2a (myosin light chain) and a-actinin at day 60 post cardiac differentiation. Right panel: The bar graphs at the right panel represent the sarcomere regularity from 3 independent experiments. Error bars represent standard deviation for the comparison between control and BTHS patient TAZ10. Significance was analyzed using Student's T-TEST **P = 0.002 and ***P = 0.0006. Scale bars: 50 µm."
NCBI gene IDs:
"TAZ: 6901"
Figure legend: "A Oxygen consumption rate (OCR) of cardiomyocytes from patient TAZ10 and control at basal conditions and after the administration of the indicated compounds."
NCBI gene IDs:
"TAZ: 6901"
Figure legend: "D Analysis of SDHA gene expression in triplicates by qPCR in cardiomyocytes, analyzed in (C)."
NCBI gene IDs:
"SDHA: 6389"
Figure legend: "A Expression  of PTPN11 (gene encoding SHP2) in skin lesions in psoriatic patients  compared with skin  from healthy donors based on microarray data (No. GSE14905). B Expression of ptpn11  in human PBMCs  of psoriatic patients (n=14) and normal controls (n=16). Data information: Data are represented mean ± SEM. P values are determined by two-tailed Mann-Whitney U test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"ptpn11: 5781
PTPN11: 5781
SHP2: 5781"
Figure legend: "G  Quantitative PCR  analysis of Ptpn11 mRNA levels in the  IMQ-treated or non-treated  dorsal  back of C57BL/6  mice  at day 5 (n=6/group). Data were normalized to Gapdh expression. Data information: Data are represented mean ± SEM. P values are determined by two-tailed unpaired Student's t-test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"Gapdh: 14433
Ptpn11: 19247"
Figure legend: "Quantitative PCR analysis of mRNA encoding  IL-23/IL-17A axis cytokines in the dorsal skin of C57BL/6 mice treated with indicated dose of SHP099  or vehicle for 4 days. Results were normalized to  Gapdh expression. Data information: Data are represented mean ± SEM. P values are determined by two-tailed unpaired Student's t-test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"Gapdh: 14433
IL-17A: 16171
IL-23: 83430"
Figure legend: "ELISA  quantification of protein levels of cytokines in mouse serum. Quantitative PCR analysis of mRNA encoding  psoriasis-related cytokines in the dorsal skin of C57BL/6 mice treated with indicated dose of SHP099  or vehicle for 4 days. Results were normalized to Gapdh  expression. Data information: Data are represented mean ± SEM. P values are determined by two-tailed unpaired Student's t-test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"Gapdh: 14433"
Figure legend: "Representative images (A and E), histological  sections of dorsal back (B and F) and clinical scores (C and G) and quantitative PCR  analysis of mRNA levels in dorsal skin  (D and H) from wild-type (n=6) and M-Shp2-/- mice  or DC-Shp2-/- mice (n=6) treated with IMQ for 4 days. Scale bar: 100 μm. Left: H&amp;E  (hematoxylin and eosin) staining data; right: statistical data (mean ± SEM). Results were normalized to Gapdh expression. Data information: Data are represented mean ± SEM. P values were calculated by Tukey multiple comparison test (B, D, F, H) or Bonferroni multiple comparison test (C, G). *P&lt;0.05, **P&lt;0.01, ns, not significant."
NCBI gene IDs:
"Gapdh: 14433
Shp2: 19247"
Figure legend: "A Heat map showing mRNA expression  in peritoneal macrophages derived from wild-type and M-Shp2-/- mice after IMQ treatment based on RNA sequencing (n=3/group). Colors represent high (red) and low (blue) intensity. Genes labeled in green indicate they are in the NF-κB signaling."
NCBI gene IDs:
"Shp2: 19247"
Figure legend: "D BMDMs and PMs derived from wild-type and M-Shp2-/- mice were stimulated by IMQ  (10 µg/ml) for indicated times. Whole cell lysates were subjected to Western blotting."
NCBI gene IDs:
"Shp2: 19247"
Figure legend: "E  Immunoblotting  of HEK293T cells co-transfected for 48 h with GFP-TLR7, plus HA-SHP2, HA-SHP2 mutant vectors, followed by immunoprecipitation with anti-GFP beads."
NCBI gene IDs:
"GFP: 
HA: 
SHP2: 5781
TLR7: 51284"
Figure legend: "F PMA-differentiated THP-1 cells were infected with vector or SHP2 lentivirus  and then treated with or without R848  (10 µg/ml). Subcellular fractionation was performed, and cytoplasmic and cell membrane proteins were probed by respective antibodies."
NCBI gene IDs:
"SHP2: 5781"
Figure legend: "G, H  Immunoblot  analysis of TLR7  and SHP2 in the isolated ER, Golgi and endosome from PMA-differentiated THP-1  cells with either SHP2 overexpressed (G) and SHP2  deficiency(H)."
NCBI gene IDs:
"SHP2: 5781"
Figure legend: "B,  Immunoblotting of TLR7  in the endosome isolated from HEK293T cells that were transfected with GFP-TLR7  or GFP-TLR7 mutant vectors."
NCBI gene IDs:
"GFP: 
TLR7: 51284"
Figure legend: "G HEK293T  cells transfected with NF-κB-luciferase  reporter and indicated TLR7  mutant vectors and then treated with  IMQ (10 µg/ml) for 24 h were harvested for luciferase assay. Data information: Data are represented mean ± SEM. P values are determined by two-tailed unpaired Student's t-test **P&lt;0.01, ns, not significant."
NCBI gene IDs:
"luciferase: 
NF-κB: 4794///84807///4793///64332///4792
TLR7: 51284"
Figure legend: "Tlr7wt/wt female mice (n=6) and Tlr7-Y1025D mutant Tlr7ki/wt female  mice (n=6) and  Tlr7ki/ki female  mice (n=5) were treated with indicated dose of IMQ for 4 days. A Phenotypic presentation (top) and H&amp;E staining (bottom) of dorsal skin. Scale bar: 100 μm. Left: H&amp;E staining data; right: statistical data (mean ± SEM). Data information: Data are represented mean ± SEM. P values are determined by two-tailed Mann-Whitney U test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"Tlr7: 170743"
Figure legend: "Tlr7wt/wt female mice  (n=6) and Tlr7-Y1025D mutant Tlr7ki/wt female mice (n=6) and Tlr7ki/ki female mice (n=5) were treated with indicated dose of IMQ  for 4 days. D Quantitative PCR analysis of mRNA encoding IL-23/IL-17A axis cytokines and other psoriasis-related cytokines in the dorsal skin. Results were normalized to  Gapdh expression. Data information: Data are represented mean ± SEM. P values are determined by two-tailed Mann-Whitney U test *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"Gapdh: 14433
Tlr7: 170743"
Figure legend: "Tlr7wt/wt female  mice (n=6) and Tlr7-Y1025D mutant Tlr7ki/wt female  mice (n=6) and Tlr7ki/ki female mice (n=5) were treated with indicated dose of IMQ  for 4 days. E Representative p-p65 staining of the dorsal skin. Scale bars: 100 μm."
NCBI gene IDs:
"Tlr7: 170743"
Figure legend: "(G) Tumour growth (in mm3) of FBXW7+/+ and FBXW7-/- xenografts in nude mice (n=10 animals per group). Treatment with either vehicle, paclitaxel (1,5mg/kg) or tigecycline (50mg/kg) started at day 6 post-tumour-injection, and was administered three times per week. Error bars indicate SEM. (H) Representative images of the xenografts defined in (G) at day 15."
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "A, The indicated cells co-transfected with p5HRE-Luc and either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (empty vector: EV) were cultured under the indicated oxygen conditions for 24 hours for the luciferase assay. B, U2OS cells co-transfected with p5HRE-Luc and the indicated siRNA were cultured under the indicated oxygen conditions for 24 hours for the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
ZBTB2: 57621"
Figure legend: "C, HCT116 p53-/- cells co-transfected with p5HRE-Luc, either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV), and either pcDNA3/p53 (at two concentrations: + and ++) or pcDNA3 (EV) were cultured under the indicated oxygen conditions for 24 hours for the luciferase assay and Western blotting using the indicated antibodies."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
p53: 7157
ZBTB2: 57621"
Figure legend: "D, HCT116 p53+/+ cells co-transfected with p5HRE-Luc, either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV), and either pcDNA3/p53 R175H, R248W, R273H, or pcDNA3 (EV) were cultured under the indicated oxygen conditions for 24 hours for the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
p53: 7157
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV) either pcDNA3/p53 (p53) or pcDNA3 (EV) (C) with the indicated siRNA were cultured under &lt; 0.1% O2 conditions for 24 hours and subjected to qRT-PCR for the indicated mRNA"
NCBI gene IDs:
"His B: 
myc: 
p53: 7157
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV) to the transwell invasion assay"
NCBI gene IDs:
"His B: 
myc: 
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with either pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV) with the indicated siRNA or to the transwell invasion assay"
NCBI gene IDs:
"His B: 
myc: 
ZBTB2: 57621"
Figure legend: "G, The number of metastatic colonies in lungs in the pulmonary metastasis model using the indicated cells stably transfected with ZBTB2 expression vector (ZBTB2) or its empty vector (EV) (left) and the representative images of lungs (right)."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "The indicated cells stably transfected with the ZBTB2 expression vector (ZBTB2) or its empty vector (EV) (H) were cultured under &lt; 0.1% O2 (H) for the indicated periods and subjected to the in vitro cell proliferation assay."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "The indicated cells stably transfected with the ZBTB2 expression vector (ZBTB2) transiently transfected with siScr or siZBTB2 (I) the indicated oxygen conditions (I) for the indicated periods and subjected to the in vitro cell proliferation assay."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "J,K, Growth of the indicated tumor xenografts with or without ZBTB2 overexpression was analyzed."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "C, Kaplan-Meier analysis of the disease-free survival of the lung cancer patients stratified by the expression levels of ZBTB2 and p53 status."
NCBI gene IDs:
"p53: 7157
ZBTB2: 57621"
Figure legend: "D-F, TCGA-based Kaplan-Meier analysis of overall survival of lung adenocarcinoma patients stratified by the expression levels of ZBTB2 and those of BAX (D), ZMAT3 (E), and CEACAM1 (F)."
NCBI gene IDs:
"BAX: 581
CEACAM1: 634
ZBTB2: 57621
ZMAT3: 64393"
Figure legend: "A, The indicated cells transiently transfected with pEF6/ZBTB2 (ZBTB2) or pEF6/myc-His B (EV) were cultured under the indicated oxygen conditions for 24 hours and subjected to Western blotting using the indicated antibodies."
NCBI gene IDs:
"His B: 
myc: 
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with both pG5H1bLuc and either pcDNA6/Gal4 DBD-HIF-1α TAD P564A pcDNA6/Gal4 DBD-HIF-1α TAD P564A and N803A (E) were additionally co-transfected with either pEF6/myc-His B (EV), pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 del[1-23] (del[1-23]), pEF6/ZBTB2 del[BTB/POZ] (del[BTB]), pEF6/ZBTB2 del[ZF1] (del[ZF1]), pEF6/ZBTB2 del[ZF2] (del[ZF2]), pEF6/ZBTB2 del[ZF3] (del[ZF3]), or pEF6/ZBTB2 del[ZF4]- (del[ZF4]), as indicated with the indicated siRNA The cells were then cultured under the indicated oxygen conditions for 24 hours and subjected to the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
Gal4: 855828
HIF-1α: 3091
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with both pG5H1bLuc and either pcDNA6/Gal4 DBD-HIF-1α TAD P564A were additionally co-transfected with either pEF6/myc-His B (EV), pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 del[1-23] (del[1-23]), pEF6/ZBTB2 del[BTB/POZ] (del[BTB]), pEF6/ZBTB2 del[ZF1] (del[ZF1]), pEF6/ZBTB2 del[ZF2] (del[ZF2]), pEF6/ZBTB2 del[ZF3] (del[ZF3]), or pEF6/ZBTB2 del[ZF4]- (del[ZF4]), as indicated with the indicated siRNA The cells were then cultured under the indicated oxygen conditions for 24 hours and subjected to the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
Gal4: 855828
HIF-1α: 3091
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with pcDNA6/Gal4 DBD-HIF-1α TAD P564A and N803A (E) were additionally co-transfected with either pEF6/myc-His B (EV), pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 del[1-23] (del[1-23]), pEF6/ZBTB2 del[BTB/POZ] (del[BTB]), pEF6/ZBTB2 del[ZF1] (del[ZF1]), pEF6/ZBTB2 del[ZF2] (del[ZF2]), pEF6/ZBTB2 del[ZF3] (del[ZF3]), or pEF6/ZBTB2 del[ZF4]- (del[ZF4]), as indicated (B,D,E,F) The cells were then cultured under the indicated oxygen conditions for 24 hours and subjected to the luciferase assay."
NCBI gene IDs:
"His B: 
myc: 
Gal4: 855828
HIF-1α: 3091
ZBTB2: 57621"
Figure legend: "The indicated cells transiently transfected with both pG5H1bLuc and either pcDNA6/Gal4 DBD-HIF-1α TAD P564A were additionally co-transfected with either pEF6/myc-His B (EV), pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 del[1-23] (del[1-23]), pEF6/ZBTB2 del[BTB/POZ] (del[BTB]), pEF6/ZBTB2 del[ZF1] (del[ZF1]), pEF6/ZBTB2 del[ZF2] (del[ZF2]), pEF6/ZBTB2 del[ZF3] (del[ZF3]), or pEF6/ZBTB2 del[ZF4]- (del[ZF4]), as indicated The cells were then cultured under the indicated oxygen conditions for 24 hours and subjected to the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
Gal4: 855828
HIF-1α: 3091
ZBTB2: 57621"
Figure legend: "H, After transient transfection with the expression vectors for ZBTB2-V5, ZBTB2-myc, or their empty vector (-), ZBTB2-myc protein was immunoprecipitated using the anti-myc antibody and co-precipitated ZBTB2-V5 was detected using the anti-V5 antibody (upper). One-tenth of the whole cell lysate (WCL) was subjected to immunoblotting with the indicated antibodies (lower)."
NCBI gene IDs:
"myc: 
V5: 
ZBTB2: 57621"
Figure legend: "I,J, The indicated cells transiently transfected with or without either pcDNA4/ZBTB2-LgBiT or pcDNA4/LgBiT and either pcDNA4/ZBTB2-SmBiT or pcDNA4/SmBiT, as indicated, were cultured under &lt; 0.1% oxygen (I) or the indicated oxygen conditions (J) and subjected to the split luciferase complementation assay."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "K, HeLa cells overexpressing wildtype or the indicated mutant of ZBTB2 (ZF2 or ZF3-deletion) were cultured under &lt; 0.1% oxygen conditions, and subjected to immunoprecipitation with anti-myc antibody. Coprecipitated DNA was subjected to the qRT-PCR experiment using primers against HRE regions of the STC1 promoter."
NCBI gene IDs:
"STC1: 6781
ZBTB2: 57621"
Figure legend: "L, The same experiment as I was conducted using the indicated combination of pcDNA4/ZBTB2-LgBiT (WT), pcDNA4/ZBTB2ΔZF2-LgBiT (ΔZF2), or pcDNA4/ZBTB2ΔZF3-LgBiT (ΔZF3), and pcDNA4/ZBTB2-SmBiT (WT), pcDNA4/ZBTB2ΔZF2-SmBiT (ΔZF2), or pcDNA4/ZBTB2ΔZF3-SmBiT (ΔZF3)."
NCBI gene IDs:
"ZBTB2: 57621"
Figure legend: "B, HCT116 p53-/- cells co-transfected with pG5H1bLuc, pcDNA6/Gal4 DBD-HIF-1α TAD P564A, and either pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 del[8-11] (ZBTB2 del[8-11]), pEF6/ZBTB2 del[51-54] (ZBTB2 del[51-54]), or pEF6/myc-His B (EV), as indicated, were cultured under &lt; 0.1% oxygen conditions for 24 hours and subjected to the luciferase assay."
NCBI gene IDs:
"His B: 
Luc: 
myc: 
Gal4: 855828
HIF-1α: 3091
p53: 7157
ZBTB2: 57621"
Figure legend: "C, After transient transfection with or without the expression vectors for ZBTB2-V5, ZBTB2 4A-V5, ZBTB2-myc, ZBTB2 4A-myc, or their empty vector, as indicated, the myc-fused proteins were immunoprecipitated using an anti-myc antibody and co-precipitated ZBTB2-V5 or ZBTB2 4A-V5 was detected (upper). One-tenth of the whole cell lysate (WCL) was subjected to immunoblotting with the indicated antibodies (lower)."
NCBI gene IDs:
"myc: 
V5: 
ZBTB2: 57621"
Figure legend: "D, HCT116 p53-/- (left) cells transiently transfected with or without either pcDNA4/ZBTB2-LgBiT (ZBTB2), pcDNA4/ZBTB2 4A-LgBiT 4A (4A), or pcDNA4/LgBiT (-) and either pcDNA4/ZBTB2-SmBiT (ZBTB2), pcDNA4/ZBTB2 4A-SmBiT (4A), or pcDNA4/SmBiT (-), as indicated, were cultured under &lt; 0.1% oxygen and subjected to the split luciferase complementation assay."
NCBI gene IDs:
"p53: 7157
ZBTB2: 57621"
Figure legend: "G, The same kind of luciferase assay to quantify the transactivation activity as B was carried out using either pEF6/ZBTB2 (ZBTB2), pEF6/ZBTB2 4A (4A), or pEF6/myc-His B (EV)."
NCBI gene IDs:
"His B: 
myc: 
ZBTB2: 57621"
Figure legend: "C. Capillary sequencing for Rnaseh2b+/+, Rnaseh2bA174T/+, and Rnaseh2bA174T/A174T DNA confirmed the presence of the introduced missense mutation."
NCBI gene IDs:
"Rnaseh2b: 67153"
Figure legend: "D. Mouse genotyping by multiplex PCR. Top: A 221 bp PCR product is present in wild-type mice (+/+); the Rnaseh2bA174T allele (also) give a 460 bp product. Bottom: Position of forward (x) and reverse primers (y, z)."
NCBI gene IDs:
"Rnaseh2b: 67153"
Figure legend: "E. Immunoblotting demonstrates depletion of all three RNase H2 protein subunits in Rnaseh2bA174T/A174T MEFs and RNASEH2BA177T/A177T LCLs. Representative of three independent experiments."
NCBI gene IDs:
"RNASEH2B: 79621
Rnaseh2b: 67153"
Figure legend: "G, H. RNase H2 enzyme activity is reduced in mouse and patient cells. (G) Enzyme activity for Rnaseh2bA174T/A174T MEFs and passage-matched Rnaseh2b+/+ controls, against RNase H substrate (RNA:DNA heteroduplex) and RNase H2-specific substrate, double stranded DNA with a single embedded ribonucleotide (DRD:DNA). Mean activity for three independent cell lines, error bars, SEM. Enzymatic activity expressed relative to the average value of control MEFs. *** = p&lt;0.001, two-tailed t-test (n=3 Rnaseh2bA174T/A174T and n=3 Rnaseh2b+/+ control MEF lines). (H) RNase H2 activity in LCLs from two independent healthy controls and an AGS patient homozygous for the RNASEH2B-A177T mutation. Enzyme activity normalised to average activity of control lines. Three independent experiments, error bars SEM. *** = p&lt;0.001 versus either control, two-tailed t-test."
NCBI gene IDs:
"Rnaseh2b: 67153
RNASEH2B: 79621"
Figure legend: "Figure 2. Increased ISG expression in tissues from Rnaseh2bA174T/A174T miceA. Transcript levels of multiple ISGs are significantly elevated in heart.B. Transcript levels of a subset of ISGs are significantly increased in kidney.C. No ISG upregulation is evident in the brain. ISG transcript levels determined by RT-qPCR normalised to transcript levels of the housekeeping gene HPRT (Oas1a was undetectable in brain). Each data point represents the mean of technical replicates of tissue RNA from a single mouse. n=9 nine-month old Rnaseh2bA174T/A174T mice, n=4 age-matched control wildtype C57BL/6Jmice. Horizontal line, mean; error bars, SEM. * = p&lt;0.05, ** = p&lt;0.01, two-tailed t-test."
NCBI gene IDs:
"HPRT: 15452
Oas1a: 246730
Rnaseh2b: 67153"
Figure legend: "A. Validation of Rnaseh2b-/- MEF lines. RER activity (DRD:DNA) is undetectable in a Rnaseh2b-/- line consistent with complete inactivation of the Rnaseh2b gene (Reijns et al, 2012). Mean of three independent experiments; error bars, SEM, **** = p&lt;0.0001, two-tailed t-test. Rnaseh2b-/- and Rnaseh2b+/+ control MEFs on a C57BL/6 p53-/- background."
NCBI gene IDs:
"Rnaseh2b: 67153
p53: 22059"
Figure legend: "B. ISG transcript levels are increased in Rnaseh2b-/- MEFs."
NCBI gene IDs:
"Rnaseh2b: 67153"
Figure legend: "C. BeadArray transcript analysis (Illumina) detected induction of multiple ISGs, including the cytokines CXCL10 and CCL5, but not other cytokine transcripts in Rnaseh2b-/- MEFs. Plotted, average fold enrichment versus p-value of significantly upregulated transcripts (p&lt;0.05, after multiple testing correction) comparing two Rnaseh2b-/- MEF lines versus 4 Rnaseh2b+/+ MEF lines. 17 out of 29 transcripts are ISGs."
NCBI gene IDs:
"CCL5: 20304
CXCL10: 15945
Rnaseh2b: 67153"
Figure legend: "D. CXCL10 and CCL5 transcripts (detected by RT-qPCR) are significantly elevated."
NCBI gene IDs:
"CCL5: 20304
CXCL10: 15945"
Figure legend: "E. Increased CXCL10 and CCL5 protein (detected by ELISA) is secreted by Rnaseh2b-/- MEFs. (B, D, E) Mean data from three experiments for six independent Rnaseh2b-/- MEF lines versus four independent Rnaseh2b+/+ MEF lines. Error bars, SEM. * = p&lt;0.05, ** = p&lt;0.01, Mann-Whitney U test."
NCBI gene IDs:
"Rnaseh2b: 67153"
Figure legend: "A. ISG activation and cytokine secretion in Rnaseh2b-/- MEFs is markedly impaired by cGAS or STING siRNA depletion. Upper left and lower panels: RT-qPCR of IFIT1, cGAS and STING transcripts, 48 h after siRNA targeting luciferase (control), cGAS or STING. Upper right panel: CCL5 is significantly reduced in culture supernatants 48 h after cGAS or STING depletion. Concentration of CCL5 (ELISA), normalised to luciferase siRNA control levels in each experiment. Mean from three independent experiments using one Rnaseh2b-/- MEF line; error bars, SEM. * = p&lt;0.05, ** = p&lt;0.01, two-tailed t-test for RT-qPCR, one sample t-test for CCL5ELISA."
NCBI gene IDs:
"luciferase: 
IFIT1: 15957
cGAS: 214763
Rnaseh2b: 67153
STING: 72512"
Figure legend: "B-D. ISG induction and cytokine secretion is abolished in Rnaseh2b-/- cGAS-/- MEFs. cGAS was targeted by CRISPR/Cas9 genome editing of a Rnaseh2b-/- MEF line to inactivate cGAS/STING signalling. In addition to sequence validation, functional inactivation of cGAS was confirmed in Rnaseh2b-/- cGas-/- CRISPR lines by the absence of CCL5 secretion in response to dsDNA (Appendix Fig S4). CCL5 (B) and CXCL10 (C) production, as well as ISG expression (D) was abrogated in Rnaseh2b-/- cGAS-/- clones, assessed by ELISA and RT-qPCR respectively. Four independent experiments, n=2 Rnaseh2b-/- cGAS-/- clones, n=4 Rnaseh2b-/- cGAS+/+ clones, error bars, SEM of each experiment * = p&lt;0.05, ** = p&lt;0.01, *** = p&lt;0.001, two-tailed t-test."
NCBI gene IDs:
"Cas9: 
CRISPR: 
cGAS: 214763
cGas: 214763
Rnaseh2b: 67153"
Figure legend: "B-D. ISG induction and cytokine secretion is abolished in Rnaseh2b-/- cGAS-/- MEFs. cGAS was targeted by CRISPR/Cas9 genome editing of a Rnaseh2b-/- MEF line to inactivate cGAS/STING signalling. In addition to sequence validation, functional inactivation of cGAS was confirmed in Rnaseh2b-/- cGas-/- CRISPR lines by the absence of CCL5 secretion in response to dsDNA (Appendix Fig S4). CCL5 (B) and CXCL10 (C) production, as well as ISG expression (D) was abrogated in Rnaseh2b-/- cGAS-/- clones, assessed by ELISA and RT-qPCR respectively. Four independent experiments, n=2 Rnaseh2b-/- cGAS-/- clones, n=4 Rnaseh2b-/- cGAS+/+ clones, error bars, SEM of each experiment * = p&lt;0.05, ** = p&lt;0.01, *** = p&lt;0.001, two-tailed t-test."
NCBI gene IDs:
"Cas9: 
CRISPR: 
CCL5: 20304
CXCL10: 15945
cGAS: 214763
cGas: 214763
Rnaseh2b: 67153
STING: 72512"
Figure legend: "E. ISG induction in Rnaseh2bA174T/A174T mice is STING dependent. RT-qPCR of RNA extracted from hearts from Sting+/+ Rnaseh2bA174T/A174T (n=4) and Sting-/-Rnaseh2bA174T/A174T (n=6) three-month old mice."
NCBI gene IDs:
"Rnaseh2b: 67153
Sting: 72512
STING: 72512"