EMBO/SourceData huggingface dataset:
Figure legend: "H PLTP protein levels in the culture conditioned medium from primary differentiated inguinal adipocytes of wild type (WT) and aP2-PRDM16 mice [24]. Peptide levels was determined by quantitative proteomics. n=2 for both groups."
NCBI gene IDs:
"PRDM16: 70673"
Figure legend: "I Transcriptional regulation of the Pltp gene by PRDM16 and PPARγ. ChIP-seq data for PRDM16 and PPARγ were obtained from [31,32], respectively."
NCBI gene IDs:
"Pltp: 18830"
Figure legend: "B Changes in body weight of mice in (A) during 10 weeks of HFD. n=9 for GFP and n=8 for PLTP (cohort 1). *P&lt;0.05, **P&lt;0.01, ***P&lt;0.01 relative to GFP by two-way ANOVA repeated measures followed by Bonferroni's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "C Changes in food intake of mice in (A) during 10 weeks of HFD (cohort 1). n=9 for GFP (cage 1 = 5 mice; cage 2 = 4 mice) and n=8 for PLTP (cage 1 = 4 mice; cage 2 = 4 mice)"
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "D Body composition of mice in (A) at 10 weeks of HFD determined by EchoMRI. n=9 for GFP and n=8 for PLTP (cohort 1). *P&lt;0.05 relative to GFP by two-way ANOVA followed by Tukey's test. N.S., not significant."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "E Whole-body energy expenditure of mice at 3 weeks of HFD. Area under the curve (AUC) of VO2 is shown in the right graph. n=7 for GFP and n=8 for PLTP (cohort 2). *P&lt;0.05 relative to GFP by Student's t-test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "F Physical movement of mice at 3 weeks of HFD as determined by metabolic cage analysis. n=7 for GFP and n=8 for PLTP (cohort 2). N.S., not significant by Student's t-test"
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "G OCR in the interscapular BAT and iWAT of control and PLTP-treated mice. One week after the treatment, BAT and iWAT were isolated from mice (7 weeks old) that received adenovirus for GFP or PLTP. n=5 for both groups. *P&lt;0.05 relative to GFP by Student's t-test. N.S., not significant."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "H Gene expression of indicated thermogenic genes in (A) from interscapular BAT for GFP (n=8) and PLTP (n=8) groups (cohort 1) except n=7 for Ucp1 of the PLTP group. Tbp was used as an internal control. *P&lt;0.05, **P&lt;0.01 relative to GFP by Student's t-test. N.S., not significant."
NCBI gene IDs:
"Tbp: 
GFP: 
PLTP: 18830
Ucp1: 22227"
Figure legend: "A Glucose tolerance test of mice that received AAV control (GFP) or PLTP at 10 weeks of HFD. After eight hours of fasting, 2 g/kg body weight of glucose was administered. n=9 for GFP and n=7 for PLTP. *P&lt;0.05, **P&lt;0.01 relative to GFP by two-way ANOVA repeated measures followed by Bonferroni's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "B Insulin tolerance test of mice in (A) at 10 weeks of HFD. Mice were fasted for four hours and administered insulin (0.75 U/kg body weight). n=9 for GFP control and n=7 for PLTP. *P&lt;0.05, **P&lt;0.01 relative to GFP by two-way ANOVA repeated measures followed by Bonferroni's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "C Fasting insulin levels of mice in (A) at 10 weeks of HFD. Blood samples were harvested after eight hours of fasting. n=5 for GFP and n=4 for PLTP. *P&lt;0.05 relative to GFP by Student's t-test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "D Glucose tolerance test of mice that received AAV control (GFP) or PLTP at 6 weeks of HFD. Note that no difference was found in body weight between the groups by two-way ANOVA repeated measures followed by Bonferroni's test.. After eight hours of fasting, 2 g/kg body weight of glucose was administered. n=7 for GFP control and n=10 for PLTP. AUC of glucose is shown in the right graph. *P&lt;0.05 by Student's t-test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "E 18F-FDG PET/CT scan images of mice that received adenovirus GFP (control) or PLTP at 14 weeks of HFD. Mice received CL316,243 at dose of 1 mg/kg of body weight 30 min before the PET/CT images. Representative images of n=5 for both groups. Yellow arrows indicate anatomical references as bladder, heart and interscapular BAT."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "F Quantification of 18F-FDG uptake in indicated tissues from mice in (E). After the 18F-FDG PET/CT imaging, indicated tissues were harvested and the radioactivity was measured by scintillation counter. n=5 for both groups. *P&lt;0.05 relative to GFP by Student's t-test. N.S., not significant."
NCBI gene IDs:
"GFP: "
Figure legend: "A Lipidomics-based quantification of indicated lipid species in mice that received tail-vein injection of adenovirus expressing PLTP or GFP (control). Blood and tissues were harvested after 10 weeks of HFD. n=4 for GFP and n=5 for PLTP in mice on HFD. LPC = (lyso)phosphatidylcholine, LPE = (lyso)phosphatidylethalonamine, PA = phosphatidic acid, SM = sphingomyelin, TAG = triglycerides, FFA = free fatty acid. Statistical analysis is demonstrated (P value) relative to GFP by Student's t-test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "B Plasma total cholesterol levels in mice that received adenovirus GFP (control) or PLTP. Blood and tissues were harvested seven days after adenovirus infection. n=8 in mice on RD and n=7 in mice on HFD for 14 weeks. ***P&lt;0.001 relative to GFP by Student's t-test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "C Relative mRNA levels of indicated genes in the liver of mice on HFD. Tbp was used as an internal control. n=4 for GFP and n=6 for PLTP. *P&lt;0.05, **P&lt;0.01 relative to GFP by Student's t-test."
NCBI gene IDs:
"Tbp: 
GFP: 
PLTP: 18830"
Figure legend: "D Bile acid levels in the liver of mice in (B). n=8 in mice on RD and n=6 in mice on HFD. *P&lt;0.05, **P&lt;0.01 relative to GFP by Student's t-test."
NCBI gene IDs:
"GFP: "
Figure legend: "E Bile acid levels in the plasma of mice in (B). n=8 in mice on RD and n=7 in mice on HFD. ***P&lt;0.001 relative to GFP by Student's t-test. F Bile acid levels in the feces of mice in (B). Feces were collected from mice on RD and HFD after seven days of treatment. n=7 in mice on RD and HFD. **P&lt;0.01, ***P&lt;0.001 relative to GFP by Student's t-test. "
NCBI gene IDs:
"GFP: "
Figure legend: "H Relative expression of Ucp1 in differentiated brown adipocytes treated with cholic acid (CA). Cells were treated with CA at indicated concentrations for 24 h. 0.1% ETOH was used as vehicle control (Ctr). n=3. Ucp1 mRNA expression was normalized by Tbp as an internal control. **P&lt;0.01 relative to Ctr by one-way ANOVA followed by Tukey's test. N.S., not significant."
NCBI gene IDs:
"Tbp: 
Ucp1: 22227"
Figure legend: "B Relative expression of Pltp in liver of mice that received adenovirus GFP (control) or PLTP. n=4 for GFP, n=6 for PLTP and n=6 for PLTPM159E. **P&lt;0.01 relative to GFP by ANOVA one-way followed by Tukey's test. N.S., not significant."
NCBI gene IDs:
"GFP: 
Pltp: 18830
PLTP: 18830"
Figure legend: "C Plasma PLTP concentration in mice that received adenovirus GFP or PLTP as determined by quantitative mass spectrometry. n=4 for GFP, n=6 for PLTP and n=6 for PLTPM159E. ***P&lt;0.001 relative to GFP by ANOVA one-way followed by Tukey's test. N.S., not significant."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "D Plasma PLTP activity in mice that received adenovirus GFP or PLTP. n=4 for GFP, n=6 for PLTP and n=6 for PLTPM159E. ***P&lt;0.001 PLTP relative to GFP and PLTPM159E; *P&lt;0.05 PLTPM159E relative to GFP by ANOVA one-way followed by Tukey's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "E Changes in body weight of mice that received AAV control or PLTP during 10 weeks of HFD. n=8 for GFP, n=10 for PLTP and n=10 for PLTPM159E. *P&lt;0.05, **P&lt;0.01 relative to PLTPM159E by two-way ANOVA repeated measures followed by Bonferroni's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "F Relative Ucp1 expression in BAT of mice that received AAV GFP (control) or PLTP at 10 weeks of HFD. n=4 for GFP, n=5 for PLTP and n=4 for PLTPM159E. Tbp was used as an internal control. *P&lt;0.05, **P&lt;0.01 by ANOVA one-way followed by Tukey's test."
NCBI gene IDs:
"Tbp: 
GFP: 
PLTP: 18830
Ucp1: 22227"
Figure legend: "G Glucose tolerance test of mice that received AAV GFP or PLTP at 10 weeks of HFD. n=5 for GFP and n=6 for both PLTP and PLTPM159E. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 PLTP relative to GFP by two-way ANOVA repeated measures followed by Bonferroni's test. AUC of glucose, shown in the right graph, was analyzed by one-way ANOVA followed by Tukey's test. N.S., not significant."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "H Plasma total cholesterol of mice that received AAV control or PLTP at 10 weeks of HFD. n=7 for all groups. *P=0.05 by ANOVA one-way followed by Fisher's LSD test. N.S., not significant."
NCBI gene IDs:
"PLTP: 18830"
Figure legend: "I Plasma bile acids in (H). n=7 for all groups. ***P&lt;0.001 PLTP relative to GFP and PLTPM159E by ANOVA one-way followed by Tukey's test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "J Plasma phospholipid in (H). n=7 for all groups. ***P&lt;0.001 PLTP and PLTPM159E relative to GFP; *P&lt;0.05 PLTP relative to PLTPM159E by ANOVA one-way followed by Fisher's LSD test."
NCBI gene IDs:
"GFP: 
PLTP: 18830"
Figure legend: "Microtubule organization in wild type stamen filament cells. (D-F) Microtubule organization in the stamen filament cells of lue1 and ktn80.1234. Arrows indicate microtubule crossovers. Dotted lines indicate abnormal microtubules."
NCBI gene IDs:
"ktn80.1: 837657
lue1: 844375"
Figure legend: "Time-lapse images showing that KTN1 is associated with microtubule severing at crossover sites in wild type filament cells. GFP-labeled KTN1 fluorescent particles are pseudo-colored in red, and microtubules (labeled with mCherry-TUB6) are shown in green. Yellow arrows indicate KTN1 at microtubule crossover sites. Asterisks indicate the location at which a microtubule has been severed at a crossover site. Time-lapse images showing that KTN1 cannot be recruited to crossover sites and no severing was observed in ktn80.1234 filament cells. Yellow open arrows indicate microtubule crossovers, which remain stable for a long time."
NCBI gene IDs:
"ktn80.1: 837657"
Figure legend: "Comparison of the morphologies of the stamen filament cells in wild type and qui-2."
NCBI gene IDs:
"qui-2: 844206///827623///824239///843845///838518"
Figure legend: "EMSA showing that BZR1 binds to the E-box of the KTN80.4 promoter regions in vitro. Nonlabelled (free) native probe in 10- to 100-fold molar excess relative to the biotin-labeled native probe was used as a cold competitor. The mutant probe was used as a negative control."
NCBI gene IDs:
"KTN80.4: "
Figure legend: "Time-lapse images showing that KTN1 is associated with microtubule severing at crossover sites in (A) wild type (B qui-2 filament cells. GFP-labeled KTN1 fluorescent particles are pseudo-colored in red, and microtubules (labeled with mCherry-TUB6) are shown in green. Yellow arrows indicate KTN1 at the microtubule crossover sites. Asterisks indicate the location at which a microtubule has been severed at a crossover site in A and B"
NCBI gene IDs:
"qui-2: 844206///827623///824239///843845///838518"
Figure legend: "Time-lapse images showing that KTN1 is associated with microtubule severing at crossover sites C) qui-2 filament cells. GFP-labeled KTN1 fluorescent particles are pseudo-colored in red, and microtubules (labeled with mCherry-TUB6) are shown in green. Yellow arrows indicate KTN1 at the microtubule crossover sites. or the location at which a microtubule has not been severed at a crossover site in C."
NCBI gene IDs:
"qui-2: 844206///827623///824239///843845///838518"
Figure legend: "(A) GAPC enzymatic activity was measured in wild-type crude extracts treated with H2O (mock), and 100 µM of Tyr-Asp, Tyr, Asp, and  Ile-Glu. (B-D) The enzymatic activities  of GAPC (B), GAPA/B (C), and GAPN  (D) were measured in the wild type and double k.o. mutant gapc1gapc2 treated with  H2O (mock), and 100 µM of Tyr-Asp. Data information: Data are mean ± SEM of n=4 (A-D; four technical replicates) , significance was assessed using unpaired two-tailed Student t-test."
NCBI gene IDs:
"gapc1: 819567
gapc2: 837904"
Figure legend: "(A-C) Fresh weight  measurements of Arabidopsis wild-type and gapc1/gapc2  double k.o. plants subjected to the oxidative stress (e.g.  catechin) and Tyr-Asp treatments. Each treatment was compared to its respective control, corresponding to plants grown in one 24-well plate (represented by the adjoined bars). (D-F) Representative Arabidopsis wild type and gapc1/gapc2  double k.o. seedlings from the different treatments. All plants were 10-day-old at the stress onset. The seedlings were exposed to  catechin for three days. Data information: Data are mean ± SEM of n=10-12 (seedlings). Unpaired two-tailed Student t-test was performed to compare treatments with control. Scale bar: 10 cm. "
NCBI gene IDs:
"gapc1: 819567
gapc2: 837904"
Figure legend: "A. Images of HeLa cells stably expressing tubulin-GFP (green) and transiently mRFP-CENP-B (magenta) treated with control siRNA (left images) and siRNA targeting PRC1 (right images). Enlargements of the boxed region, shown to the right of the image of the whole spindle, are focused on kinetochores and bridging fiber (top: merge; middle: GFP, bottom: scheme)."
NCBI gene IDs:
"PRC1: 9055"
Figure legend: "B. Western blot showing PRC1 protein from unlabeled cells, tubulin-GFP HeLa cell line and PRC1-GFP HeLa cell line. Detailed measurements are in Expanded View 4."
NCBI gene IDs:
"PRC1: 9055"
Figure legend: "C. Mean ratio of signal intensities of the bridging fiber (Ib, measured at the position of a blue line as in scheme) and sum of the bridging and k-fiber (Ibk, measured at the position of the orange line as in scheme), measured in control cells (grey bar) and PRC1 siRNA treated cells (black bar) (p=0.0003).D. Mean interkinetochore distance (dk, see scheme) measured in control cells (grey bar) and PRC1 siRNA treated cells (black bar) (p=0.0001)."
NCBI gene IDs:
"PRC1: 9055"
Figure legend: "F. Mean signal intensity I of the immunostained PRC1 in control cells (grey bar) and PRC1 siRNA treated cells (black bar) in HeLa cells expressing tubulin-GFP and immunostained for PRC1 (p=0.0001)."
NCBI gene IDs:
"PRC1: 9055"
Figure legend: "G. Images of HeLa cells stably expressing PRC1-GFP treated with control (left image) and siRNA targeting PRC1 (right image) immunostained for PRC1 (magenta). Graph showing the length of the immunostained PRC1 signal, LPRC1 (p=0.5167), signal intensity, I (p=0.0003) and Icross (p=0.0001) in siRNA targeting PRC1 in comparison to control HeLa cells expressing PRC1-GFP and immunostained for PRC1.H. Images of HeLa cells stably expressing PRC1-GFP (green) treated with control (left image) and siRNA targeting PRC1 (right image) immunostained for PRC1. Graph showing the length of the PRC1-GFP signal, LPRC1 (p=0.3407), signal intensity, I (p=0.0001), and Icross (p=0.0001) in siRNA targeting PRC1 in comparison to control HeLa cells expressing PRC1-GFP and immunostained for PRC1."
NCBI gene IDs:
"PRC1: 9055"
Figure legend: " C To determine whether autophosphorylation occurs in cis or trans, autophosphorylation was measured for WT or a kinase dead form of FAK (KD, contains K454R mutation) in presence or absence of a substrate deficient FAK mutant (Y397F) and/or PI(4,5)P2 vesicles. PI(4,5)P2 negative experiments contain control PC vesicles instead. Note that the scales of the two plots are different, i.e. autophosphorylation is increased in presence of PI(4,5)P2. Plotted are mean values from two independent phosphorylation reactions, each determined in duplicates (n=4). Left: Schematic of possible cis- and trans-autophosphorylation (red arrows) in mixtures of FAK-WT/FAK-Y397F and FAK-KD/FAK-Y397F. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " D Src phosphorylation of Y577 in FAK in presence of PC or PI(4,5)P2 vesicles as determined by an ELISA method. The plot on the left is obtained in presence Src containing only the kinase domain (Src254-536) and the plot on the right with Src containing additionally the SH3 and SH2 domains (Src84-536). Plotted are mean values from two independent phosphorylation reactions, each determined in duplicates (n=4). "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " Interactions between WT or interface mutant FAK with a PI(4,5)P2 containing membrane was probed using a pulldown assay with membrane coated silica beads. Plotted are bound protein relative to the binding for WT at the highest PI(4,5)P2 concentration. Mutant naming is as in Table 2. For WT the non-specific binding to a PC membrane is shown for comparison. Plotted are mean values from at least three independent experiments. Error bars: s.d. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " A FAK phosphorylation of WT or interface mutant FAK in SCC cells. Whole cell lysates were subjected to western blot analysis with anti-pFAK Y397, anti-pFAK Y576/577, anti-pFAK Y861, anti-pFAK Y925 and anti-FAK. Anti-GAPDH served as a loading control. The graph shows densitometric analysis of relative pFAK/FAK ratios (mean) from three independent experiments. Student's t-test was carried out to calculate the statistical significance. Error bars: s.d. * = p &lt; 0.01, # = p &lt; 0.05. For full blots see source data. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " B Downstream signaling in SCC cells expressing WT or interface mutant FAK. Whole cell lysates are analyzed by western blot analysis with the indicated antibodies. The graph shows densitometric analysis of relative phospho/total protein ratios (mean) from three independent experiments. Student's t-test was carried out to calculate the statistical significance. Error bars: s.d. * = p &lt; 0.01, # = p &lt; 0.05. For full blots see source data. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: "FAK-expressing SCC cells were grown on glass coverslips, fixed and stained with anti-FAK anti-Paxillin and DAPI Representative immunofluorescence images are shown. Scale bars, 20 μm."
NCBI gene IDs:
"FAK: 396416"
Figure legend: " B FAK-expressing SCC cells were grown on glass coverslips, fixed and stained with anti-pSrc Y416 , anti-Paxillin and DAPI Representative immunofluorescence images are shown. Scale bars, 20 μm. The graph (B) shows the quantification of internalized active Src from three independent experiments. Student's t-test was carried out to calculate the statistical significance. Error bars, s.d. * = p &lt; 0.01. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " C Subcellular fractionation of SCC cells expressing FAK-WT or interface mutant FAK. Whole cell lysates (WCL) and subcellular fractions were subjected to western blot analysis with anti-FAK, anti-Tubulin (marker for cytoplasmic fraction; C), anti-RCAS (marker for perinuclear fraction; PN) and anti-H4 (marker for nuclear fraction; N). The graph shows densitometric analysis of relative FAK amount of the FAK mutants in the different fractions from three independent experiments. Student's t-test was carried out to calculate the statistical significance. Error bars: s.d. * = p &lt; 0.01, # = p &lt; 0.05. For full blots see source data. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " A FAK-WT, interface mutant FAK or FAK -/- SCC cells were resuspended in methylcellulose solution in growth medium on a layer of agarose and observed for 3D sphere formation. Images were taken from 10 random fields after nine days (representative images are shown) and the relative colony size (top graph) as well as the number of colonies (bottom graph) were assessed from three independent experiments. Scale bars, 200 μm. Student's t-test was carried out to calculate the statistical significance. Error bars s.d. * = p &lt; 0.01, # = p &lt; 0.05. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: " B FAK-WT, interface mutant FAK or FAK -/- SCC cells were seeded on growth factor-reduced Matrigel in serum-free media. After 72 h, invasion towards a serum gradient was analyzed by staining the cells with calcein and taking images at 10 μm increments through the matrigel. Representative images of the invasion assay are shown. Scale bars, 200 μm. The graph shows relative invasion from five independent experiments. Student's t-test was carried out to calculate the statistical significance. Error bars, s.e.m. * = p &lt; 0.01, # = p &lt; 0.05. "
NCBI gene IDs:
"FAK: 396416"
Figure legend: "Example images showing the localization of SOX2-SNAP and OCT4-HALO by fluorescence microscopy of SBROS cells. Scale bar: 50µm."
NCBI gene IDs:
"OCT4: 18999"
Figure legend: "Correlation between OCT4-HALO and total OCT4 levels determined by immunofluorescence and HALO labelling. R is Pearson's correlation coefficient."
NCBI gene IDs:
"OCT4: 18999"
Figure legend: "Distributions of OCT4 (n=2682), SOX2 (n=1236) and NANOG (n=2416) levels in WT E14 and SBROS cell lines as determined by quantitative immunofluorescence. Dashed lines: mean protein levels; Dotted lines: median protein levels."
NCBI gene IDs:
"OCT4: 18999"
Figure legend: "Protein half-lives of OCT4-HALO and SOX2-SNAP in SBROS cells. Whiskers: minimum and maximum values; Box: lower and upper quartiles; Solid line: median. Black dots: outliers."
NCBI gene IDs:
"OCT4: 18999"
Figure legend: "Endogenous SOX2 levels in single cells (turquoise) normalized to the value at t=0 in SNSF cells upon induction of SOX2-SNAP (n=37, left) or a truncated SOX2 version missing the DNA binding domain (YPET-SOX2-delDBD, n=47, right). Black line: average SOX2-SNAP levels after induction normalized to the maximum level (n=22); Yellow line: average YPET-SOX2-delDBD levels after induction, normalized to the maximum level (n=20)."
NCBI gene IDs:
"SNAP: 
YPET: 
SOX2: 20674"
Figure legend: "Sox2 and Oct4 mRNA levels upon overexpression of YPet-SOX2 or YPET-SOX2-delDBD (n=3). p-values: two-sided t-test with unequal variance."
NCBI gene IDs:
"YPet: 
YPET: 
Oct4: 18999
Sox2: 20674
SOX2: 20674"
Figure legend: "Changes of OCT4 half-life upon SOX2-SNAP or YPET-SOX2-delDBD overexpression (n=20 cells each). p-values: Mann-Whitney U test. SBROS cells harbouring inducible SOX2-SNAP or YPET-SOX2-delDBD were treated with 0 or 500 ng/ml dox for 6-8 hours followed by pulse-labelling of OCT4-HALO and live-cell imaging."
NCBI gene IDs:
"SNAP: 
YPET: 
SOX2: 20674"
Figure legend: "Differentiation outcome of uninduced cells or cells induced 12 hours for OCT4-SNAP overexpression before sorting in G1 phase (n=6). p-values: two-sided t-test with unequal variance."
NCBI gene IDs:
"SNAP: 
OCT4: 18999"
Figure legend: "C. cGAMP-VLPs induce an IFN-I response in target cells. HEK293 cells were infected with decreasing amounts of cGAMP-VLPs and Empty-VLPs (1/5 serial dilutions starting at 2μL of VLP stocks per well) and the infection was monitored 24 hours later by quantifying GFP+ cells by flow cytometry. Supernatants from the same infected cells were then transferred to a reporter cell line expressing firefly luciferase under a promoter induced by IFN-I (ISRE). Luciferase activity measured 24 hours later indicated the presence of IFN-I in the supernatants."
NCBI gene IDs:
"luciferase: "
Figure legend: "C. Enhanced antibody production following immunisation with cGAMP-VLPs relies on STING signalling. WT or Sting-/- mice were immunised with PBS, cGAMP-VLPs, Empty-VLPs or Empty-VLPs + poly(I:C). IgG2b antibodies recognising VLP proteins were assessed by ELISA. The optical density at increasing serum dilutions is shown in the first two graphs from the left and the EC50 is on the right."
NCBI gene IDs:
"Sting: 72512"
Figure legend: "A qRT-PCR analysis of LINC00941 repression during keratinocyte differentiation in primary keratinocyte cultures from three different donors compared to undifferentiated keratinocytes (D0) (n = 6 biological replicates). Data is presented as mean ± SD."
NCBI gene IDs:
"LINC00941: 100287314"