EMBO/SourceData huggingface dataset:
Figure legend: "(B) Enhanced resistance of Gmlmm1-1 and Gmlmm1-2 to Psg infection. Leaves from 2-week-old soybean plants were infected with Psg and the bacteria number was determined at 0 and 4 dpi (Mean ± SD, n = 8, n represents sample number, ***, p &lt; 0.001, Student's t-test). Data information: The experiments were performed three times , as biological replicates, with similar results."
NCBI gene IDs:
"Gmlmm1: 100499640"
Figure legend: "(E) Subcellular localization of GmLMM1-GFP and GmLMM1L407H-GFP in N. benthamiana. PI4P indicates the membrane localization. Scale bar, 10 μm. GFP, green fluorescent protein. Data information: The experiments were performed three times as biological replicates, with similar results."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: "(A) ROS accumulation in Gmlmm1-1 and Gmlmm1-2 mutant plants. Soybean leaves (aged 3 weeks) were subjected to ROS accumulation examination by DAB staining. Scale bar, 1 cm. Data information: The experiments were performed three times as biological replicates, with similar results. The exact number (n) and p values are shown in the source data."
NCBI gene IDs:
"Gmlmm1: 100499640"
Figure legend: "(A) Flg22-induced ROS burst in Gmlmm1-1, Gmlmm1-2 and Williams 82. The indicated leaves (aged 2 weeks) were subjected to flg22-induced ROS examination, and the peak relative luminescence unit (RLU) value was recorded (Mean ± SEM, n ≥ 12, n represents sample number). Data information: All the experiments were performed three times (biological replicates) with similar results. The exact n, SEM values and p values are shown in the source data."
NCBI gene IDs:
"Gmlmm1: 100499640"
Figure legend: "(B) Flg22-induced ROS burst in the CRISPR lines C9-24-13 and DN50. The indicated leaves (aged 2 weeks) were subjected to flg22-induced ROS examination, and the relative RLU value was recorded (Mean ± SEM, n ≥ 9, n represents sample number). Data information: All the experiments were performed three times (biological replicates) with similar results. The exact n, SEM values and p values are shown in the source data."
NCBI gene IDs:
"CRISPR: "
Figure legend: "(C) Suppression of flg22-induced ROS production in Arabidopsis by GmLMM1. The GmLMM1 gene was introduced into Arabidopsis WT (Col-0) plants by Agrobacterium-mediated transformation. Two independent T2 lines were subjected to flg22-induced ROS examination (Mean ± SD, n ≥8, n represents sample number, ***, p &lt; 0.001, Student's t-test). The protein expression is shown. Data information: All the experiments were performed three times (biological replicates) with similar results. The exact n, SEM values and p values are shown in the source data."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: "Suppression of flg22-induced (D) ROS production in N. benthamiana by GmLMM1. The indicated constructs were transiently expressed by Agrobacterium-mediated transient expression for 2 days and subjected to flg22-induced ROS examination. GmLMM1K564E contains a mutation at the predicted ATP binding site without kinase activity. GFP was used as a control (Mean ± SD, n = 6, n represents sample number, **, p &lt; 0.01, ***, p &lt; 0.001, Student's t-test). The protein expression is shown in the lower panel. Data information: All the experiments were performed three times (biological replicates) with similar results. The exact n, SEM values and p values are shown in the source data."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: "E) Suppression of chitin-induced (E) ROS production in N. benthamiana by GmLMM1. The indicated constructs were transiently expressed by Agrobacterium-mediated transient expression for 2 days and subjected to ROS examination. GmLMM1K564E contains a mutation at the predicted ATP binding site without kinase activity. GFP was used as a control (Mean ± SD, n = 6, n represents sample number, **, p &lt; 0.01, ***, p &lt; 0.001, Student's t-test). The protein expression is shown in the lower panel. Data information: All the experiments were performed three times (biological replicates) with similar results. The exact n, SEM values and p values are shown in the source data."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: "(F) Inhibition of XEG1-induced cell death in N. benthamiana by GmLMM1. The indicated constructs were transiently expressed in N. benthamiana 1 day before infiltration of Agrobacterium containing the XEG1 or GFP vector. The cell death phenotypes were visualized 5 days later. Protein expression of GmLMM1-HA and GmLMM1K564E-HA is shown in the lower panel."
NCBI gene IDs:
"GmLMM1: 100499640
XEG1: 20663425"
Figure legend: "(A) Identification of GmLMM1-interacting proteins by IP-MS analysis. GmLMM1-FLAG was transiently expressed in Arabidopsis protoplasts, purified by anti-FLAG M2 agarose, and subjected to mass spectrometry analysis. The complete list is shown in the source data."
NCBI gene IDs:
"FLAG: 
GmLMM1: 100499640"
Figure legend: "(B) Interactions of GmLMM1 with PRR receptors. The indicated constructs were transiently expressed in N. benthamiana leaves and subjected to luciferase complementation assay. GmFLS2 (Gm08G083300) is the closest homolog of FLS2 in soybean. GmILPA1 was used as a negative control. The protein interaction intensity is shown by the relative luminescence unit (RLU) (Mean±SD, n ≥ 6, n represents sample number, p&lt;0.05, Student's t-test, different letters indicate signiﬁcant difference). Immunoblots show the levels of protein expression. Data information: The experiments were performed three times as biological replicates, with similar results."
NCBI gene IDs:
"FLS2: 
Gm08G083300: 
GmILPA1: 
GmFLS2: 100817960"
Figure legend: "(C) Suppression of flg22-induced NbFLS2-NbBAK1 interaction by GmLMM1. NbFLS2 and NbBAK1 were co-expressed in N. benthamiana along with GmLMM1 or GmLMM1L407H, treated with H2O or flg22 and subjected to co-IP assays. Data information: The experiments were performed three times as biological replicates, with similar results."
NCBI gene IDs:
"BAK1: 
FLS2: 
GmLMM1: 100499640"
Figure legend: "(D Suppression effect of GmLMM1 on the flg22-induced interaction of GmFLS2 with GmBAK1a (D) Data information: The experiments were performed two times (D as biological replicates, with similar results."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: "E) Suppression effect of GmLMM1 on the flg22-induced interaction of GmFLS2 with GmBAK1b (E). Data information: The experiments were performed two times E), as biological replicates, with similar results."
NCBI gene IDs:
"GmLMM1: 100499640"
Figure legend: " In ThP1 cells co-infected with a HIV1-GFP virus and a HIV1-LUC virus, nuclear foci contain mixtures of both viruses. (D) Dual-color image shows RNA-FISH against GFP (red) and RNA-FISH against LUC (green). Arrows show foci containing both GFP vRNA and LUC vRNA. The bottom row shows the boxed region magnified, with GFP vRNA and LUC vRNA displayed separately and in combination. See Appendix Fig. S7. "
NCBI gene IDs:
"GFP: 
LUC: "
Figure legend: " (F) Scatter plot shows intensities of GFP RNA and LUC RNA in colocalizing foci with the Spearman correlation r and associated p-value. "
NCBI gene IDs:
"GFP: 
LUC: "
Figure legend: "(B) Green image shows RNA-FISH against NEAT1."
NCBI gene IDs:
"NEAT1: 283131"
Figure legend: " (D) Boxplots show Pearson correlations r between vDNA (EdU) and CPSF6, NEAT1 or SC35 in 17-19 regions of interest (ROIs). Central lines in boxes define medians, top and bottom limits define upper and lower quartiles, respectively. Whiskers show the full data range, except for outliers. Grey dots are individual data points. Significance of positive or negative correlations was assessed using the Costes method of random ROI shifts. Highly significant (p&lt;0.01) positive correlations between vDNA and CPSF6 intensities are found in 16 out of 19 ROIs; highly significant negative correlations between vDNA and NEAT1 are found in 19/19 ROIs, and highly significant positive correlations between vDNA and SC35 are found for 16/17 ROIs. Data are from one experiment. For CPSF6, the experiment was repeated with similar results. "
NCBI gene IDs:
"NEAT1: 283131"
Figure legend: " The left panel shows the timeline of infection and/or drug exposure experiments. (A-D) Percentage of GFP positive cells at 3 and 7 days post-infection analyzed by FACS in absence of EdU. (A) Uninfected control cells. (B) Untreated, infected ThP1 cells fixed at 3 d (72 h) p.i. (C) Infected cells were exposed to NVP for 72 h, then NVP was washed out and cells were cultured for another 96 h and fixed at 7 d p.i. (D) Infected cells were cultured for 72 h p.i., then NVP was washed out and cells were exposed to the nuclear import inhibitor PF74 for another 96 h and fixed at 7 d p.i. (E-H) Multicolor images of ThP1 cells infected with a GFP-reporter virus. vRNA (RNA-FISH) is labeled in red, GFP in green, and nuclei (DAPI) in blue. (E) Uninfected control cells. (F) Untreated, infected ThP1 cells fixed at 3 d (72 h) p.i. (G) Infected cells were exposed to NVP for 72 h, then NVP was washed out and cells were cultured for another 96 h and fixed at 7 d p.i. (H) Infected cells were cultured for 72 h p.i., then NVP was washed out and cells were exposed to the nuclear import inhibitor PF74 for another 96 h and fixed at 7 d p.i. The experiment was repeated three times. "
NCBI gene IDs:
"GFP: "
Figure legend: " The left panel shows the timeline of infection and/or drug exposure experiments. (A-D) Multicolor images of vDNA, vRNA, DAPI and/or GFP in monocyte derived macrophages (MDMs) from two different donors, infected with HIV1. Cells from donor 1 were infected with VSV-G-pseudotyped HIV-1ΔEnv Vpx carrying a GFP reporter (A) and cells from donor 2 were infected with a VSV-G-pseudotyped HIV-1ΔEnv without Vpx (B-D). (A,B) Infected cells were left untreated and fixed at 6 d p.i. (C) Infected cells were treated with 10 µM Nevirapine (NVP) throughout the experiment and fixed at 6 d p.i. (D) Infected cells were exposed to NVP for 3 d p.i., then NVP was washed out and cells were cultured for another 4 d and fixed at 7 d p.i. Arrows in (A-D) show the position of selected vRNA foci. (E) Boxplot shows the size of DNA foci in untreated infected cells from donor 2 imaged at 7 d p.i. (B), measured by the FWHM of n=24 foci. The red line defines the median, top and bottom limits define upper and lower quartiles, respectively. Whiskers show the full data range, except for outliers. Grey dots are individual data points. (F) Boxplot shows Pearson's r, measuring colocalization of DNA with RNA foci in the same cells (n=20). Center line defines the median, top and bottom limits define upper and lower quartiles, respectively. Whiskers show the full data range, except for outliers. Grey dots are individual data points. "
NCBI gene IDs:
"GFP: 
Env: 155971
Vpx: 1490006"
Figure legend: "Boxplots (central band = median, boxes = Quartiles, whiskers = 1.5 * interquartile range), illustrating the differences in GE180 PET (A) compared between wild type C57BL/6 mice (n=43) and APPNL-G-G (n=15)."
NCBI gene IDs:
"APP: 11820"
Figure legend: "B Boxplots (central band = median, boxes = Quartiles, whiskers = 1.5 * interquartile range), illustrating the differences in the rate of change in Florbetaben PET (B) compared between wild type C57BL/6 mice (n=43) and APPNL-G-G (n=15)."
NCBI gene IDs:
"APP: 11820"
Figure legend: "A-C Coronal (top row) and axial (bottom row) slices of average 18-F GE-180 TSPO (microglia) tracer and 18F-florbetaben amyloid-PET split up by group (A - C): C57BL/6 (A), APPNL-G-F mice subgroup with a low (&lt; median) GE-180 PET (B), and the APPNL-G-F mice subgroup with a high (&gt; median) GE-180 PET (C). APPNL-G-F mice with low baseline GE180 PET levels showed faster increase in Florbetaben PET between 5 and 10 months (B) compared to those at high baseline level of GE-180 PET (C)."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(B-D) Kaplan-Meier survival  plots comparing the survival  probability of all melanoma patients  (B), BRAF-mutated patients  (C) and BRAF  wild-type (WT) patients  (D) with high- (magenta) and low (black) MCUA mRNA expression."
NCBI gene IDs:
"BRAF: 673
MCU: 90550"
Figure legend: "(E-G) Kaplan-Meier survival  plots comparing the survival  probability of patients  of indicated cancer types that are divided into either high- (magenta) or low (black) MCUA mRNA expression. P-values were calculated by using a log-rank test. Abbreviations: KICH  = kidney chromophobe; KIRC  =  kidney renal clear cell carcinoma; KIRP  = kidney renal papillary cell carcinoma; LUAD  = lung adenocarcinoma; LUSC  =  lung squamous cell carcinoma; PAAD  = pancreatic adenocarcinoma."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(C) Representative images of the migrated stained 1205Lu  shCTRL (black frame), shMCU_1 (darker blue frame) and shMCU_2 (lighter blue frame) cells on the lower side of the insert. (D) Quantification of the transwell migration in 1205Lu  stable MCUA_KD lines, based on the number of stained  cells (n=4 biological replicates/condition, shown also by individual data points). Data information: Data are presented as mean ± SEM. Statistical significance was determined using unpaired, two‐tailed Student's t‐test (shMCU cells were compared to their respective control, shCTRL), (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(E) Representative images of 1205Lu  shCTRL (black frame), shMCU_1 (darker blue frame) and shMCU_2 (lighter blue frame) melanoma spheroids  after 72 h invasion  in collagen. Live cells   are shown in green. Scale bar: 100 µm. (F) Quantification of 1205Lu  stable  MCUA_KD spheroid core size (n=6 biological replicates/condition, shown also by individual data points). Data information: Data are presented as mean ± SEM. Statistical significance was determined using unpaired, two‐tailed Student's t‐test (shMCU  cells were compared to their respective control, shCTRL), (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(G) Representative images of WM3734  shCTRL (black frame), shMCU_1 (darker orange frame) and shMCU_2 (lighter orange frame) melanoma spheroids. Live cells are shown in green. Scale bar: 100 µm. (H) Quantification of WM3734  stable MCUA_KD spheroid core size  (n=5 biological replicates/condition, shown also by individual data points). Data information: Data are presented as mean ± SEM. Statistical significance was determined using unpaired, two‐tailed Student's t‐test (shMCU cells were compared to their respective control, shCTRL), (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(L) Representative images of 451Lu  wild-type (grey frame), 451Lu-BR3 (lighter green frame), WM983B  wild-type (grey frame) and WM983B-BR (darker green frame) melanoma spheroids   after 72 h invasion  in collagen. Live cells  are shown in green. Scale bar: 100 µm. (M-N) Quantification of 451Lu  versus 451Lu-BR3 (M) and WM983B  versus  WM983B-BR (N)  spheroid core size   (n≥9 biological replicates/condition, shown also by individual data points). Data information: Data are presented as mean ± SEM. Statistical significance was determined using unpaired, two‐tailed Student's t‐test (shMCU  cells were compared to their respective control, shCTRL), (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(A-C) Mitochondrial hydrogen peroxide  (H2O2) measurement in 1205Lu  and WM3734  with and without stable  MCUA_KD using mito-HyPer. Exemplary ratiometric images (F505 nm/F420 nm) are shown for all conditions (A). Scale bar: 10 µm. Quantification of mito-HyPer  ratio in 1205Lu  (B) and WM3734  (C) under resting state (1205Lu  ─ shCTRL: n=217 cells from 6 biological replicates; shMCU_1: n=197 cells from 7 biological replicates; shMCU_2: n=212 cells from 7 biological replicates; WM3734  ─ shCTRL: n=104 cells from 6 biological replicates; shMCU_1: n=107 cells from 7 biological replicates; shMCU_2: n=114 cells from 7 biological replicates). Data information: All data are presented as mean ± SEM. and were measured in Ringer's buffer containing 0.5 mM Ca2+  statistical significance was determined using unpaired, two‐tailed Student's t‐test, (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005. In all statistical analyses, KD cells were compared to their respective control."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(D-F) Cytosolic hydrogen peroxide (H2O2) measurement in 1205Lu  and WM3734  with and without stable MCUA_KD using HyPer. Exemplary ratiometric images (F505 nm/F420 nm) are shown for all conditions (D). Scale bar: 10 µm. Quantification of HyPer  ratio in 1205Lu  (E) and WM3734  (F) under resting state (1205Lu  ─ shCTRL: n=232 cells from 6 biological replicates; shMCU_1: n=290 cells from 6 biological replicates; shMCU_2: n=290 cells from 6 biological replicates; WM3734 ─ shCTRL: n=443 cells from 9 biological replicates; shMCU_1: n=297 cells from 9 biological replicates; shMCU_2: n=233 cells from 9 biological replicates). Data information: All data are presented as mean ± SEM. and were measured in Ringer's buffer containing 0.5 mM  Ca2+ statistical significance was determined using unpaired, two‐tailed Student's t‐test, (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005. In all statistical analyses, KD cells were compared to their respective control."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(I-K) Mitochondrial glutathione redox potential, measured with mito-Grx1-roGFP2 in 1205Lu  and WM3734  with and without stable MCUA_KD. Representative ratiometric images (F385 nm/F475 nm) are shown for all conditions (I). Scale bar: 10 µm. Quantification in 1205Lu  (J) and WM3734  (K) under resting state (1205Lu ─ shCTRL: n=231 cells from 10 biological replicates; shMCU_1: n=176 cells from 7 biological replicates; shMCU_2: n=164 cells from 7 biological replicates; WM3734  ─ shCTRL: n=127 cells from 7 biological replicates; shMCU_1: n=142 cells from 7 biological replicates; shMCU_2: n=116 cells from 7 biological replicates). Data information: All data are presented as mean ± SEM. and were measured in Ringer's buffer containing 0.5 mM Ca2+ statistical significance was determined using unpaired, two‐tailed Student's t‐test, (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005. In all statistical analyses, KD cells were compared to their respective control."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(G-I) Mitochondrial ATP, measured using mito-ATEAM  in 1205Lu  and WM3734  with and without stable MCUA_KD. Exemplary ratiometric images (FRET/CFP) are shown for all conditions (G). Scale bar: 10 µm. Quantification of basal levels in 1205Lu  (H) and WM3734  (I) (1205Lu  ─ shCTRL: n=141 cells from 14 biological replicates; shMCU_1: n=143 cells from 12 biological replicates; shMCU_2: n=143 cells from 13 biological replicates; WM3734  ─ shCTRL: n=122 cells from 12 biological replicates; shMCU_1: n=103 cells from 12 biological replicates; shMCU_2: n=99 cells from 13 biological replicates). Data information: Statistical significance was determined using unpaired, two‐tailed Student's t‐test, (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "(J) Exemplary confocal microscope  images of the   mitochondrial network  (blue: DAPI staining  of the nucleus; green: TOMM20 staining  of mitochondria); scale bar: 50 µm. (K-O)  Electron microscopy of mitochondria  of stable  MCUA_KD cell lines. (K) Exemplary images of stable  MCUA_KD cells' mitochondria. Scale bar: 500 nm. (L-O) Quantification of mitochondrial  diameter (L and N) and intercristae  distance (M and O) of 1205Lu  (L and M) and WM3734  (N and O) cells with and without stable MCUA_KD, presented as boxplot. The box presents the 25 %- quartile, median and 75 %-quartile, the X represents the mean and the whiskers the minimum and maximum, outliers are represented as dots (mitochondrial  diameter: 1205Lu  ─ shCTRL: n=47 technical replicates; shMCU_1: n=44 technical replicates; shMCU_2: n=40 technical replicates; WM3734  ─ shCTRL: n=35 technical replicates; shMCU_1: n=52 technical replicates; shMCU_2: n=38 technical replicates; intercristace  distance: 1205Lu  ─ shCTRL: n=330 technical replicates; shMCU_1: n=285 technical replicates; shMCU_2: n=255 technical replicates; WM3734  ─ shCTRL: n=352 technical replicates; shMCU_1: n=433 technical replicates; shMCU_2: n=357 technical replicates). Data information: Statistical significance was determined using unpaired, two‐tailed Student's t‐test, (*) p &lt; 0.05; (**) p &lt; 0.01; (***) p &lt; 0.005; no asterisk means no statistical significance (p &gt; 0.05)."
NCBI gene IDs:
"MCU: 90550"
Figure legend: "B, qRT-PCR of piwi-1 relative to GAPDH in RNAi animals as indicated, 2dpi. Circles represent individual biological replicates averaged from 3 technical replicates."
NCBI gene IDs:
"GAPDH: 
piwi-1: "
Figure legend: "D, Percentage of X1 cells from control and atm(RNAi) animals, 1dpi or unirradiated."
NCBI gene IDs:
"atm: "
Figure legend: "E, qRT-PCR of piwi-1 relative to GAPDH in RNAi animals as indicated, 2dpi. Data are averages ± SD; circles represent biological replicates; statistics represent ordinary one-way ANOVA compared to controls, ****P&lt;0.0001, ns=not significant."
NCBI gene IDs:
"piwi-1: 
GAPDH: "
Figure legend: "F, piwi-1 fluorescent in situ hybridization (FISH) of RNAi animals as indicated, 2 dpi."
NCBI gene IDs:
"piwi-1: "
Figure legend: "A, Top: Strategy for EdU pulse and animal fixation. Bottom: Confocal images of EdU-stained atm and control RNAi animals. B, Quantification by area of EdU+ cells from A. Circles represent individual animals. Inset, expanded scale showing compressed data below. Data includes two independent experiments."
NCBI gene IDs:
"atm: "
Figure legend: "D, Confocal images of phosphohistone H3 (pH3)-stained RNAi animals after radiation exposure. Blue, DAPI. E and F, Quantification by area of pH3 cells in radiated (E) or unirradiated (F) animals after atm (solid line) and control (dashed line) RNAi."
NCBI gene IDs:
"atm: "
Figure legend: "A, Confocal images of piwi-1 FISH in RNAi animals after radiation exposure. Scale bars=250µm. B and C, Percentage of animal area occupied by piwi-1 FISH divided by total animal area, in radiated (B) or unirradiated (C) RNAi animals. D, Total number of piwi-1+ cells in RNAi animals, 7 dpi. Experiments in (A) - (D) were repeated twice."
NCBI gene IDs:
"piwi-1: "
Figure legend: "A - C, Kaplan-Meier survival curves of intact RNAi animals after radiation, n = 50 animals, repeated twice. Experiments in A and B were performed simultaneously but split into two graphs for clarity; control and atm(RNAi) values are replicated in both panels."
NCBI gene IDs:
"atm: "
Figure legend: "D, Confocal images of piwi-1 FISH in RNAi animals after exposure to 20Gy, 2dpi. Scale bars=250µm. E, Quantification by area of piwi-1+ cells in RNAi animals after 20Gy exposure, 7dpi."
NCBI gene IDs:
"piwi-1: "
Figure legend: "Western blotting for  mTORC1 and mTORC2 activity in  mESCs transduced with Rptor  and Rictor  shRNAs."
NCBI gene IDs:
"Rictor: 78757
Rptor: 74370"
Figure legend: "Images of  mESCs  transduced with Rptor and Rictor  shRNAs in 2iL. Scale bar, 400 µm.   Relative  proliferation of mESCs as in (E) (mean values ± SD, P value was calculated using two-tailed unpaired Student's t test, *P &lt; 0.05, also hereafter in similar experiments). n = 3 biological replicates.  "
NCBI gene IDs:
"Rictor: 78757
Rptor: 74370"
Figure legend: "Images of mESCs transduced with Rptor  siRNA on different days in 2iL. siNC, transduced with a negative control; day 0 was the day of siRNA transfection, also hereafter in similar experiments. Scale bar, 400 µm."
NCBI gene IDs:
"Rptor: 74370"
Figure legend: "RNA-seq  heatmaps of the indicated pluripotency gene sets in control and INK128 (200 nM) treated mESCs in  2iL.   RNA-seq heatmaps of indicated pluripotency gene sets in Rptor WT and KO mESCs  in 2iL.  "
NCBI gene IDs:
"Rptor: 74370"