EMBO/SourceData huggingface dataset:
Figure legend: " D Kaplan-Meier survival analysis of NSG mice inoculated i.v. with SUM159PT gNFIB shCTRL (n = 9), SUM159PT gNFIB sh1 ERO1A (n = 7), or SUM159PT gNFIB sh2 ERO1A (n = 7) cells. Two-tailed log-rank test. "
NCBI gene IDs:
"ERO1A: 30001
NFIB: 4781"
Figure legend: " E Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases 8 (SUM159PT gNFIB shCTRL) and 20 (SUM159PT gNFIB sh1 ERO1A or SUM159PT gNFIB sh2 ERO1A) days after i.v. injection of the respective cells. n = 4, means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"ERO1A: 30001
NFIB: 4781"
Figure legend: " F Kaplan-Meier survival analysis of overall survival of mice injected i.v. with SUM159PT gNFIB shCTRL (n = 10), SUM159PT gNFIB sh1 ERO1A (n = 12), or SUM159PT gNFIB sh2 ERO1A (n = 13) cells, two-tailed log-rank test. "
NCBI gene IDs:
"ERO1A: 30001
NFIB: 4781"
Figure legend: " A Bar graph showing the ROS levels in the NFIB models. The fluorescence signal was normalized to the blank. n = 2 biological replicates and n = 2 technical replicates, means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"NFIB: 4781"
Figure legend: " C Left panel: Bar graph representing mean VEGFA mRNA expression in SUM159PT gCTRL and SUM159PT gNFIB breast cancer cell lines upon downregulation of ERO1A with two independent shRNAs (sh1 and sh2). Right panel: Bar graph representing mean VEGFA mRNA expression in lung extracts from animals inoculated i.v. with SUM159PT gNFIB shCTRL, SUM159PT sh1 ERO1A, or SUM159PT sh2 ERO1A cells. In both graphs n = 3 biological replicates and n = 2 technical replicates, means ± s.d., two-tailed Student's t-test, FC= fold change. "
NCBI gene IDs:
"ERO1A: 30001
NFIB: 4781
VEGFA: 7422
VEGFA: 22339"
Figure legend: " D Left panel: Representative images of CD31-positive endothelial structures in lungs. Scale bar, 1 mm. Right panel: Bar graphs showing lung metastases quantified as percentage of metastatic area per lung area and quantification of CD31 staining in the metastatic area. Means ± s.d., n = 8 shCTRL, n = 5 per sh1 ERO1A, and n = 6 sh2 ERO1A, two-tailed Student's t-test."
NCBI gene IDs:
"ERO1A: 30001"
Figure legend: " E Left panel: Bar graph showing average of master segments counted after incubation of HUVEC cells with conditioned medium from LM1, HR1, 4T1 (Nfib high-expression and the respective Nfib low-expression or KO controls) and SUM159PT gNFIB shCTRL, SUM159PT gNFIB sh1 ERO1A, SUM159PT gNFIB sh2 ERO1A cells. Human VEGF121(0.4 ng/μl) was added to SUM159PT gNFIB sh1 and sh2 ERO1A cells, n = 2 technical replicates, means ± s.d., two-tailed Student's t-test. Right panel: Representative images showing tube formation four hours after addition of conditioned medium from HR1 gCtrl or g4Nfib.Scale bar 100 μm. "
NCBI gene IDs:
"ERO1A: 30001
Nfib: 18028
NFIB: 4781"
Figure legend: "
 C Higher NFIB expression in iC10 and basal breast cancer subtype compared with the other subtypes using intClust (Dawson et al, 2013) and PAM50 classifications in the METABRIC cohort (n = 1980 across the 10 Intclust, iC). Boxplot represents NFIB expression values comprised between the 1st and the 3rd quartiles with the central band representing median expression value, and whiskers indicating the farthest data points that are still within the distance of 1.5 times the interquartile range (IQR). 
"
NCBI gene IDs:
"NFIB: 4781"
Figure legend: " D, E Distant metastasis-free survival (D, n = 232) and overall survival (E, n = 241) plots generated using the Kaplan-Meier Plotter based on the mean expression using the signal intensity of the NFIB (213029_at) combined with the ERO1A (218498_s_at) probes. The cut-off was automatically set to split patients into two groups (median), high and low. The plots were generated using the signal intensity of the different probes in Affymetrix microarray gene expression data from TNBC patients of The Cancer Genome Atlas. Number of patients (n) and P values (two tailed log-rank test) are presented in the panels. "
NCBI gene IDs:
"ERO1A: 30001
NFIB: 4781"
Figure legend: "Whole-mount fluorescence view of E14.5-15.5 hearts from Npr3-sCreER;R26-tdTomato and Npr3-CreER;R26-tdTomato mice. Immunostaining for tdTomato, ESR and CDH5 on heart sections. C.M., compact myocardium; T.M., trabecular myocardium. Arrowheads, tdTomato+ESR- endocardial cells."
NCBI gene IDs:
"tdTomato: 
Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "Isolation of tdTomato- or tdTomato+ cells from E14.5 Npr3-sCreER;R26-tdTomato and Npr3-CreER;R26-tdTomato mice by FACS."
NCBI gene IDs:
"tdTomato: 
Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "qRT-PCR of Cre, ESR, Npr3 and Cdh5 from tdTomato+ cells. Data are mean ± s.e.m.; n = 5; *P &lt; 0.05."
NCBI gene IDs:
"Cdh5: 12562
Cre: 2777477
ESR: 13982
Npr3: 18162"
Figure legend: "Whole-mount fluorescence images of E14.5 Npr3-CreER;R26-Confetti and Npr3-sCreER;R26-Confetti mouse hearts. No Tam is used as control for leakiness of Npr3-sCreER."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "Immunostaining for CDH5 on heart sections shows a significantly increase of endothelial cell labeling in Npr3-sCreER;R26-Confetti heart compared with Npr3-CreER;R26-Confetti heart (Tam). *P &lt; 0.05; Data are mean ± s.e.m.; n = 5. Scale bars, 500 µm in c; 100 µm in d. Each image is representative of 5 individual biological samples."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "Whole mount fluorescent images of E14.5 or E15.5 Npr3-sCreER;R26-LZLT and Npr3-CreER;R26-LZLT mouse hearts. No tam is as control for detecting leakiness of sCreER or CreER. Representative Immunostaining images for tdTomato, ESR and CDH5 on heart sections."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "Quantification of the percentage of endocardial cells expressing tdTomato in Npr3-sCreER;R26-LZLT (sCreER) or Npr3-CreER;R26-LZLT (CreER) hearts. Data are mean ± s.e.m.; n = 5; *P &lt; 0.05. Scale bars, 500 µm. Each figure is a representative of 5 individual biological samples."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
R26: 14910
Npr3: 18162"
Figure legend: "Immunostaining for VEGFR2 on Npr3-sCreER;Kdrflox/flox heart section from mice without tamoxifen treatment (No Tam)."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
Kdr: 16542
Npr3: 18162"
Figure legend: "Whole-mount fluorescence images of Col1a2-sCreER;R26-tdTomato or Col1a2-CreER;R26-tdTomato hearts. Inserts indicate bright-field images."
NCBI gene IDs:
"tdTomato: 
Col1a2: 12843
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "Immunostaining for tdTomato and ESR on heart sections. Arrowheads indicate valve mesenchyme. Quantification of the percentage of PDGFRa+ cells expressing tdTomato in Col1a2-sCreER;R26-tdTomato (sCreER) or Col1a2-CreER;R26-tdTomato (CreER) hearts. n.s., non-significant; Data are mean ± s.e.m.; n = 6; n.s., non-significant. Quantification of the percentage of tdTomato+ cells expressing ESR. *P &lt; 0.05; Data are mean ± s.e.m.; n = 6; *P &lt; 0.05."
NCBI gene IDs:
"tdTomato: 
Col1a2: 12843
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "Immunostaining for PDGFRa in heart sections shows a significant increase of fibroblast labeling in valves of Col1a2-sCreER;R26-Confetti heart compared with Col1a2-CreER;R26-Confetti heart (Tam). Quantification of the percentage of fibroblasts expressing fluorescent reporters in cardiac valves. Data are mean ± s.e.m.; n = 5; *P &lt; 0.05. Scale bars, 500 µm in c-e; 100 µm in h. Each figure is a representative of 5-6 individual biological samples."
NCBI gene IDs:
"Col1a2: 12843
Cre: 2777477
ER: 2099
R26: 14910"
Figure legend: "(D-F) Box plots showing diastolic (D) and systolic (E) diameters and cardiac  contractility analyses (percent fractional shortening) (F) performed using SOHA   approach for controls (Hand-Gal4  and UAS-dmiR-1 sponge) and Hand&gt;sponge  dmiR-1 contexts at ages  one and five weeks. n = 20 hearts. Central band corresponds to median. Whiskers correspond to Min to Max. Box corresponds to interquartile range from 25th to 75th percentile. Data information: Scale bar = 20 µm. p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (**) p-value =0.021, (***) p-value =0.0002. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Gal4: 855828
Hand: 34379
miR-1: 12798026
sponge: 43404"
Figure legend: "(G,G') Representative spot views generated using Imaris from  in situ hybridization with miRCURY  LNA probe for dmiR-1  and used for quantification of dmiR-1  levels. Spot views of dmiR-1 in hearts  of one-week-old control (UAS-mblRNAi) (G) and DM1 flies  (Hand&gt;mblRNAi) (G') are shown. Each spot represents a pool of dmiR-1 transcripts detected in the same area. The zoom region in G corresponds to area in which FISH  signal was quantified. Data information: Scale bar = 40 µm."
NCBI gene IDs:
"Hand: 34379
mbl: 36945
miR-1: 12798026"
Figure legend: "(H, I) Scatter plot graph showing the signal intensity quantified in cardioblasts  of one- and five-week-old flies  for controls (UAS-Bru3, UAS-mblRNAi) and DM1  contexts (Hand&gt;Bru3, Hand&gt;mblRNAi). n = 9 hearts. Bars corresponds to mean ± SD. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (**) p-value =0.021, (***) p-value =0.0002. (****) p-value&lt;0.0001. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Bru3: 39527
Hand: 34379
mbl: 36945"
Figure legend: "(C-F') dmiR-1 binding site in 3'UTR-Mp  region is required to negatively regulate Mp expression in vivo. Adult hearts  from transgenic GFP-sensor lines carrying 3'UTRMp region with (UAS-GFP  3'UTRMp) or without dmiR-1 seed site (UAS-GFP  ∆3'UTRMp). These lines were combined with the UAS-dmiR1 line to generate double transgenic lines and tested for GFP expression  in non-targeted context (crossed with w1118) (C,E) or in heart-targeted context (crossed with heart  specific driver Hand-Gal4) (D,F). When crossed with Hand-Gal4, GFP expression  in Hand&gt;GFP  3'UTRMp; dmiR1 line (carrying dmiR-1 seed site) (D') is attenuated in cardiac tube and  pericardial cells compared to Hand&gt;GFP  ∆3'UTRMp; dmiR-1 line (lacking dmiR-1 seed site) (F'). Scale bar = 40 µm."
NCBI gene IDs:
"GFP: 
Gal4: 855828
Hand: 34379
miR-1: 12798026
miR1: 12798026
Mp: 38769"
Figure legend: "(K,L) Graphs of Mp  signal quantification in cardioblasts  from adult flies aged  one and five weeks for controls (UAS-mblRNAi, UAS-Bru3) and DM1  contexts (Hand&gt;mblRNAi, Hand&gt;Bru3) using CTCF  method. For each genotype 9 hearts  were analyzed and fluorescence intensity was measured in two regions from segment A3 and two regions from segment A4. Bars correspond to mean ± SD. Data information: p-value&lt;0.05 considered statistically significant; (*) p-value = 0.033; (**) p-value = 0.021; (***) p-value = 0.0002; (****) p-value&lt;0.0001. Non-parametric test Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Bru3: 39527
Hand: 34379
mbl: 36945"
Figure legend: "(E-G) Box plots showing cardiac  variables (diastolic (E) and systolic (F) diameters) and percent fractional shortening (G) for controls (Hand-Gal4  and UAS-Mp) and Mp  overexpression (Hand&gt;Mp) conditions at one and five weeks. Notice an increase in diastolic  and systolic  diameters  with reduced cardiac  contractility in Hand&gt;Mp  in comparison to controls. n = 20 hearts. Central band corresponds to median. Whiskers correspond to Min to Max. Box corresponds to interquartile range from 25th to 75th percentile. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (**) p-value =0.021, (***) p-value =0.0002. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Gal4: 855828
Hand: 34379
Mp: 38769"
Figure legend: "(A-B') Adult heart  of 1 week of age labeled for Mp  (green) and actin  (red) for control (UAS-Mp  RNAi) (A,A') and mutant (Hand&gt;Mp  RNAi) contexts (B,B'). Data information: Scale bar = 20µm."
NCBI gene IDs:
"Hand: 34379
Mp: 38769"
Figure legend: "(C) Fluorescence signal intensity quantification for Mp expression  in cardioblasts  in adult heart  of 1 week for control (UAS-Mp  RNAi) and mutant (Hand&gt;Mp  RNAi) contexts using CTCF  method. For each genotype 9 hearts  were analyzed and fluorescence intensity was measured in two regions from segment A3 and two regions from segment A4. Bars correspond to mean ± SD. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (**) p-value =0.021, (***) p-value =0.0002. (****) p-value&lt;0.0001. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Hand: 34379
Mp: 38769"
Figure legend: "(D-F) Box plots showing cardiac  size analyzes (diastolic  (D) and  systolic  diameters (E)) and percent fractional shortening (F) performed by SOHA   approach for controls (Hand-Gal4  and UAS-Mp  RNAi) and mutant (Hand&gt;Mp  RNAi) contexts at 1 and 5 weeks of age, showing reduced size of cardiac tube in Hand&gt;Mp  RNAi context in comparison to the Hand-Gal4  control. n = 20 hearts. Central band corresponds to median. Whiskers correspond to Min to Max. Box corresponds to interquartile range from 25th to 75th percentile. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (**) p-value =0.021, (***) p-value =0.0002. (****) p-value&lt;0.0001. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest."
NCBI gene IDs:
"Gal4: 855828
Hand: 34379
Mp: 38769"
Figure legend: "(A, B) (A) Col15A1   transcript levels tested by  RT-qPCR and (B) Col15A1  protein levels tested by Western blot in cardiac  samples from healthy donors and from DM1 patients  with (DCM+) and without DCM   (DCM-). Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (***) p-value =0.0002. (****) p-value&lt;0.0001. Parametric t-test was performed to compare control samples and samples of interest in A, B"
NCBI gene IDs:
"Col15A1: 1306"
Figure legend: "(C) miR-1 levels tested by RT-qPCR in cardiac  samples from healthy donors and from DM1 patients. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (***) p-value =0.0002. (****) p-value&lt;0.0001. Parametric t-test was performed to compare control samples and samples of interest in C."
NCBI gene IDs:
"miR-1: 406904"
Figure legend: "(D, F) Box plots showing cardiac  variables (diastolic (D) and systolic (E) diameters and percent fractional shortening (F)) assessed by SOHA  approach for controls (UAS-Bru3; Mp  RNAi and UAS-UPRT; Bru3), Mp  rescue (Hand&gt;Bru3; Mp  RANi) and DM1  context (Hand&gt;UPRT; Bru3) at five weeks of age  showing rescue of diastolic   and systolic  diameters in Hand&gt;Bru3; Mp  RNAi in comparison to controls (D,E) and rescue of cardiac  contractility (F) n = 20 hearts. Central band corresponds to median. Whiskers correspond to Min to Max. Box corresponds to interquartile range from 25th to 75th percentile. Data information: p-value&lt;0.05 considered statistically significant. (*) p-value = 0.033, (***) p-value =0.0002. (****) p-value&lt;0.0001. Non-parametric Mann Whitney test was performed to compare control samples and samples of interest in D,E,F."
NCBI gene IDs:
"UPRT: 
Bru3: 39527
Hand: 34379
Mp: 38769"
Figure legend: "B, C Both  mRNA and protein levels of RBM47 were elevated in SeV-infected (B) or IFN-α-stimulated (C) 293T,  HFF, HUVEC, and THP-1  cells. Cells were infected with SeV (MOI = 1) or stimulated with IFN-α for 6 or 12 h, and the expression of RBM47 mRNA  (at 6 and 12 h post treatment) and protein (at 6 and 12 h post treatment) were tested. The PCR  results are represented as the means ± SD of n = 3 biological replicates. Data information: Data are representative of n = 3 independent experiments. NS, non-significant; * P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test)."
NCBI gene IDs:
"RBM47: 54502"
Figure legend: "C, D Levels of  viral genome, protein, and  progeny of VSV (C) or HSV-1 (D) were quantified in virus-infected  293T cells with or without RBM47 ectopic expression at the indicated time points. The viral protein levels were tested 12 h post VSV infection and 24 h post HSV-1  infection.The PCR  and viral titer data are represented as the means ± SD of n = 3 biological replicates. Data information: The data shown are representative of n = 3 independent experiments.**P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test)."
NCBI gene IDs:
"RBM47: 54502"
Figure legend: "D, E mRNA expression of IFIT1, ISG15, and Cig5 in  SeV-infected  293T  cells overexpressing  RBM47 (D) or RBM47  knocked down cells (E). Cells were infected with SeV for 24 h, and  gene expression was determined using qRT-PCR.The PCR results are represented as the means ± SD of n = 3 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; * P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test)."
NCBI gene IDs:
"IFIT1: 3434
ISG15: 9636
RBM47: 54502
Cig5: 91543"
Figure legend: "F The expression of  IFIT1, ISG15, and  Cig5 in RBM47-overexpressing or control  293T  cells stimulated by  IFN-α for 12 h. The PCR results are represented as the means ± SD of n = 3 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; * P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test)."
NCBI gene IDs:
"IFIT1: 3434
ISG15: 9636
RBM47: 54502
Cig5: 91543"
Figure legend: "G The  expression of  IFIT1, ISG15, and Cig5 in WT and RBM47+/- peritoneal  macrophages stimulated by  IFN-α for 12 h. The PCR results are represented as the means ± SD of n = 3 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; * P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test). "
NCBI gene IDs:
"IFIT1: 15957
ISG15: 100038882
RBM47: 245945
Cig5: 58185"
Figure legend: "B IFNAR1 mRNA stability after DRB treatment. 293T cells were transfected with  RBM47-Flag or empty vectors and treated with DRB (50 μg/mL). Total RNA was harvested at 0, 15, 30, 45, and 60 min, and IFNAR1 mRNA levels were measured using qRT-PCR and normalized to  β-actin. The PCR results are represented as the means ± SD of n = 3 biological replicates. Data information: Data are representative of n = 3 independent experiments. NS, non-significant; **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test)."
NCBI gene IDs:
"Flag: 
β-actin: 60
IFNAR1: 3454
RBM47: 54502"
Figure legend: "A mRNA of  IFNAR1, but not IFNAR2  or STAT1, was stabilized by  RBM47 ectopic expression in 293T cells with or without IFN-α  stimulation. Cells were treated with IFN-α or  PBS for 6 h, and the relative gene expression levels were determined using qRT-PCR.The PCR  results are represented as the means ± SD of n = 3 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test). The data shown are representative of three independent experiments."
NCBI gene IDs:
"IFNAR1: 3454
IFNAR2: 3455
RBM47: 54502
STAT1: 6772"
Figure legend: "F, G  Protein levels of IFNAR1 and phosphorylation levels of TYK2, STAT1, and STAT2 in RBM47-overexpressing (F) or knockdown (G) 293T  cells. Cells were treated with PBS or IFN-α for 6 h, and the relative band densities were analyzed using ImageJ software and normalized to GAPDH. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test). The data shown are representative of three independent experiments."
NCBI gene IDs:
"RBM47: 54502"
Figure legend: "G RBM47+/+ and RBM47+/- mice were infected with HSV-1 (1× 107 pfu) via caudal vein injection. Viral genome DNA and progeny titers were tested using blood samples from infected mice. Data are represented as the means ± SD of n = 6 biological replicates. H qRT-PCR analysis of IFNAR1 and Cig5 mRNA in blood samples from HSV-1-infected  mice. The PCR results are represented as the means ± SD of n = 6 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; * P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test). "
NCBI gene IDs:
"IFNAR1: 15975
RBM47: 245945
Cig5: 58185"
Figure legend: "D The mRNA levels of VSV and RBM47  in blood  samples of  IFNAR1 deficient (IFNAR1-/-) mice (n = 6 per group) infected with VSV. Mice were infected with shmRBM47 or control lentivirus for 7 days and then challenged with VSV  for another 24 h. Each dot represents one  mouse. The  PCR results are represented as the means ± SD of n = 6 biological replicates. Data information: The data shown are representative of n = 3 independent experiments. NS, non-significant; **P ≤ 0.01, and ***P ≤ 0.001 (Student's t-test). "
NCBI gene IDs:
"IFNAR1: 15975
RBM47: 245945"
Figure legend: "Plot showing the average Z-scores of 210 endolysosomal genes plotted against the negative log10 of the P values from the one-sample t-test of whether average Z-score ≠ 0. Green circles represent significantly upregulated genes (Z &gt; 0 and P &lt; 0.05). Magenta circles represent significantly downregulated genes (Z &lt; 0 and P &lt; 0.05). Grey circles represent genes whose expression is not significantly changed (P &gt; 0.05). All comparisons were between cancers and normal cells from the same tissue. Dashed line shows position on Y-axis that corresponds to P = 0.05. Positions of MCOLN1 (red circle), VAC14 (blue circle), and MTM1 (yellow circle) are indicated. Inset, schematic showing that Mtm1 and Vac14 regulate the levels of PI(3,5)P2, and thereby, influence TRPML1 activity."
NCBI gene IDs:
"MCOLN1: 57192
MTM1: 4534
VAC14: 55697"
Figure legend: "Bar graph showing the relative expression of the indicated genes in the indicated cell types. Values were normalized to respective 'HN31+HRAS shRNA' average and represent mean±SEM. Data points represent values from biological replicates. Statistical test employed was Student's t-test."
NCBI gene IDs:
"HRAS: 3265"
Figure legend: "Western blots performed on extracts isolated from the indicated cells probed with antibodies against Tfeb and tubulin. Molecular weights are shown on the left. Bar graph shows relative Tfeb levels in the indicated samples. Values were normalized to 'HN31+HRAS shRNA' and represent mean±SEM. Data points represent values from biological replicates. Statistical test employed was Student's t-test."
NCBI gene IDs:
"HRAS: 3265"
Figure legend: "Bar graph showing the relative expression of MCOLN1 in the indicated cell types. Values were normalized to HT1197 average and represent mean±SEM. Data points represent values from biological replicates. Statistical test employed was Student's t-test."
NCBI gene IDs:
"MCOLN1: 57192"
Figure legend: "Representative images showing Ki67-labeling in xenografts generated from implantation of HN31 cells stably expressing control shRNA (left) or MCOLN1 shRNA (right). Pseudocolored panels show the representative distribution Ki67 positive or negative nuclei. Scale bars shown (0.5 mm) apply to all panels."
NCBI gene IDs:
"MCOLN1: 57192"
Figure legend: "Bar graphs showing the relative cell numbers in UMSCC-22A-HRASWT cells following the indicated perturbations. All values represent mean±SEM. Data points represent values from biological replicates. Statistical test employed was Student's t-test."
NCBI gene IDs:
"HRAS: 3265"
Figure legend: "Kaplan-Meier curves showing the survival of patients stratified on the basis of MCOLN1 expression in BLCA and HNSC patients whose tumors were wild-type for HRAS (right) or carried oncogenic HRAS mutations (left). Statistical test employed was the Mantel-Cox log-rank test."
NCBI gene IDs:
"HRAS: 3265
MCOLN1: 57192"