EMBO/SourceData huggingface dataset:
Figure legend: "(A) Degradation of Orm2 depends on Dfm1 and not Der1. The indicated strains expressing Orm2-RFP were grown into log phase and degradation was measured by cycloheximide chase (CHX). After CHX addition, cells were lysed at the indicated times, and analyzed by SDS-PAGE and immunoblotted for Orm2-RFP with α-RFP (3 biological replicates (n=3). (B) Same as (A) except degradation of Orm2-RFP was measured in WT and der1∆ cells (3 biological replicates; n=3)."
NCBI gene IDs:
"der1: 852500
Der1: 852500
Dfm1: 852020"
Figure legend: "(D) Dfm1's SHP box is not required for degradation of Orm2-RFP. In the indicated strains, degradation of Orm2-RFP was measured by CHX-chase assay. Cells were analyzed by SDS-PAGE and immunoblotted for Orm2-RFP with α-RFP (3 biological replicates; n=3)."
NCBI gene IDs:
"Dfm1: 852020"
Figure legend: "(G) Dfm1's WR motif, GxxxG motif, substrate binding and lipid thinning function are required for degradation of Orm2-RFP. In the indicated strains, degradation of Orm2-RFP was measured by CHX-chase assay. Cells were analyzed by SDS-PAGE and immunoblotted for Orm2-RFP with α-RFP (3 biological replicates; n=3)."
NCBI gene IDs:
"Dfm1: 852020"
Figure legend: "(H) Dfm1 L1 residues are required for binding to Orm2. Orm2-RFP and binding to retrotranslocation-deficient Dfm1 L1 mutants was analyzed by co-IP. The IP was analyzed for presence of Dfm1-HA. As a negative control, cells not expressing Orm2-RFP were used (3 biological replicates; n=3). Band intensities for all western blots were normalized to PGK1 loading control and quantified by ImageJ."
NCBI gene IDs:
"Dfm1: 852020
Orm2: 851064"
Figure legend: "(A) E3 ligase Tul1 is required for Orm2 degradation. In the indicated strains, degradation of Orm2-RFP was measured by CHX-chase assay. Cells were analyzed by SDS-PAGE and immunoblotted for Orm2-RFP with α-RFP (3 biological replicates; n=3). t=0 was taken as 100% and data is represented as mean ± SEM."
NCBI gene IDs:
"Tul1: 853832"
Figure legend: "(B) Cdc48 and the proteasome are required for Orm2 degradation. Same as (A), except cdc48-2 and hrd2-1 were analyzed for Orm2-RFP degradation (3 biological replicates; n=3). t=0 was taken as 100% and data is represented as mean ± SEM."
NCBI gene IDs:
"cdc48: 851431
hrd2: 856422"
Figure legend: "(C) Dfm1 does not function in the post-ubiquitination step of Orm2 degradation pathway. Indicated strains expressing Orm2-RFP were grown into log phase. Cells were lysed, and microsomes were collected and immunoprecipitated with α-RFP conjugated to agarose beads. Samples were then subjected to SDS-PAGE and immunoblot by α-Ubiquitin and α-RFP (3 biological replicates; n=3)."
NCBI gene IDs:
"Dfm1: 852020"
Figure legend: "(D) ERAD mutants do not rescue temperature-sensitive lethality of tsc3∆. Indicated strains were grown to log-phase in SC and serially diluted cultures were plated on SC plates and incubated at 37°C and imaged at Day 2 (3 biological replicates, 2 technical replicates; n=5)."
NCBI gene IDs:
"tsc3: 852350"
Figure legend: "(A) Phosphorylated Orm2 accumulates in the absence of Dfm1. Phos-tag western blot analysis shows that there is an accumulation of phosphorylated Orm2 in dfm1∆ cells. The indicated strains were grown to log phase, treated with vehicle or 1.5 μM myriocin for 1 hour and subjected to SDS-PAGE or Phos-tag western blot analysis via blotting for Orm2 with α-RFP and PGK1 with α-PGK1 antibodies (2 biological replicates; n=2)."
NCBI gene IDs:
"Dfm1: 852020
dfm1: 852020
Orm2: 851064"
Figure legend: "(C) dfm1∆ cells block the degradation of phosphorylated mimic of Orm2 (Orm2-3D). In the indicated strains, degradation of Orm2-3A-GFP and Orm2-3D-GFP was measured by CHX-chase assay. Cells were analyzed by SDS-PAGE and immunoblotted α-GFP (3 biological replicates; n=3). t=0 was taken as 100% and data is represented as mean ± SEM."
NCBI gene IDs:
"dfm1: 852020"
Figure legend: "(A) Dfm1-HA binding to Orm2-GFP, Orm2-3A-GFP, Orm2-3D-GFP, Orm1, Lcb1, and Lcb2 were analyzed by co-IP. As negative control, cells not expressing Dfm1-HA were used. Also, Sec61 was also included to test for non-specific binding (3 biological replicates; n=3). (B) Same as (A), except co-IP was performed on WT and orm2∆ cells and Dfm1-HA binding to Orm1, Lcb1, and Lcb2 was analyzed (3 biological replicates; n=3). (C) Same as (A), except Orm2-GFP binding to Dfm1-HA, Orm1, Lcb1, and Lcb2 was analyzed by co-IP (3 biological replicates; n=3)."
NCBI gene IDs:
"GFP: 
HA: 
Dfm1: 852020
orm2: 851064
Orm2: 851064"
Figure legend: "(a) Fold change in gene expressions of CXCL10, CXCL11, CXCL12, CCL20 and CX3CL1 in NAFLD (n = 10) BOECs, normalized to healthy (n = 12) BOECs, with 2 technical replicates per donor. (c) Fold change in gene expressions of CXCL10, CXCL11, CXCL12, CCL20 and CX3CL1 in LPS+FFA-treated or non-treated NAFLD (n = 5) and healthy (n = 5) BOECs, normalized to non-treated healthy BOECs, with 3 technical replicates per donor."
NCBI gene IDs:
"CCL20: 6364
CX3CL1: 6376
CXCL10: 3627
CXCL11: 6373
CXCL12: 6387"
Figure legend: "(a) Upper panel: Single-cell UMAP of sequenced PBMCs from 2 NAFLD patients and 4 healthy subjects; n = 10,786 cells. We identified 15 distinct cell types by DICE annotation. Lower panel: Expression patterns of CXCR3, CXCR4, ACKR3, CX3CR1, CCR6 across different cell types in NAFLD and healthy PBMC samples."
NCBI gene IDs:
"ACKR3: 57007
CCR6: 1235
CX3CR1: 1524
CXCR3: 2833
CXCR4: 7852"
Figure legend: "A) IFNγ  cytokine measured in the amniotic fluid  (AF)  and fetal liver supernatant  (FL) of IFNγKO (IFNγ-/-) mice  one day following injection of cytokine on E14.5. ND = not detectable. Data represent mean of 3 fetuses/condition and error bars represent + SD."
NCBI gene IDs:
"IFNγ: 15978"
Figure legend: "C-H) Total cellularity of C) HSPCs, D) LT-HSCs, E) ST-HSCs, F) MPP2, G) MPP3, and H) MPP4  in saline  or IFNγ  exposed littermates of each genotype (IFNγ  +/- or -/-), as derived from the cross (IFNγR +/- dam). n = 19-23 fetuses  from 3 litters/condition."
NCBI gene IDs:
"IFNγ: 15979
IFNγR: 15979"
Figure legend: "J-O) Total cellularity of J) HSPCs, K) LT-HSCs, L) ST-HSCs, M) MPP2, N) MPP3, and O) MPP4  in saline  or IFNγ  exposed littermates of each genotype (IFNγ  +/- or -/-), (IFNγR  -/- dam). n = 19-21 fetuses  from 3 litters/condition. For all analysis bars represent mean. Statistical significance was determined by unpaired student's t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001."
NCBI gene IDs:
"IFNγ: 15979
IFNγR: 15979"
Figure legend: "(B) Quantitative PCR analysis of post-natal lungs for mitochondrial DNA (mtDNA). Actin was used as a normalization control. (mean ± SD, : p&lt;0.05;: p&lt;0.01; N=3)."
NCBI gene IDs:
"Actin: "
Figure legend: "(D) Quantitative analysis of the efficiency of NHEJ-repair in a reporter cell line, as described in Methods. Cells were cultured in low glucose (5.5mM) or high glucose (30mM) for 5 days, fructose (30mM), ribose (20mM), for 3 days (graph bar 4). I-SceI, untransfected cells served as a negative control. Shown is the average from three independent experiments (mean ± SEM, : p&lt;0.01 : p&lt;0.001)."
NCBI gene IDs:
"I-SceI: 854590"
Figure legend: "(A) Representative images of γH2AX positive nuclei in lungs of 6-month STZ induced diabetic mice, transduced with respective RAGE virions as described in Methods. The lungs were harvested 6-weeks after viral transduction. The empty vector served as control. Red represents γH2AX foci green cytoplasmic staining represents α-tubulin; blue nuclear staining represents DAPI (Scale 10µm). No primary antibody control served as a negative control (from control lung) of the staining."
NCBI gene IDs:
"RAGE: 11596"
Figure legend: "(C) Representative images of lungs from 6-month STZ induced diabetic mice, transduced with respective RAGE virions as described in Methods. Lungs were stained for cellular senescence-associated β-galactosidase as described in Figure 5a and visualized by bright field and polarized light, where the accumulated senescent areas are recognized by its blue staining (Scale 40µm)."
NCBI gene IDs:
"RAGE: 11596"
Figure legend: "(E) Quantitative analysis of Masson's Trichrome stain for lungs of 6-month STZ induced diabetic mice with respective RAGE virions as described in Figure 5F (mean ± SD, : p&lt;0.001; N=8)."
NCBI gene IDs:
"RAGE: 11596"
Figure legend: "(F) Representative images of Masson's Trichrome stain for lungs transduced with the respective RAGE expressing virions as described in Figure 5a and visualized by bright field and polarized light, where the accumulated ECM areas are recognized by its blue staining (Scale 40µm). The dotted lines represent the respective zoomed window."
NCBI gene IDs:
"RAGE: 11596"
Figure legend: "(G) Pressure-volume curves of diabetic and non-diabetic mice were determined using the FlexiVent system 6 weeks after transduction using respective the RAGE virions, as described in Figure 5a. The curves represent group averages (N=8). The lungs studied in Figure 5a, b, c and d are identical (mean ± SD; Vector alone vs. RAGE-EE; : p&lt;0.05; N=8)."
NCBI gene IDs:
"RAGE: 11596"
Figure legend: "J-L) mRNA expression of p21 (j), IL-6 (k) and IL-8 (l) 20 days after neutrophil co-culture; Data are mean±S.E.M. of 2-3 independent experiments."
NCBI gene IDs:
"p21: 1026
IL-8: 3576
IL-6: 3569"
Figure legend: "i) Comparison between p16Ink4a mRNA levels of young (3 months old) and old INK-ATTAC mice (28-29 month old) treated with vehicle or AP20187. Data are mean ± SEM of n = 5-8 mice per age group; j) % of TAF positive hepatocytes in old INK-ATTAC mice (28-29 months old) treated with vehicle or AP20187. Data are mean ± SEM of n = 5-6 mice per group; k) Liver neutrophils (Ly6G) in old INK-ATTAC mice (28-29 months old) treated with vehicle or AP20187. Data are mean ± SEM of n = 5-7 mice per group; "
NCBI gene IDs:
"p16Ink4a: 12578"
Figure legend: "A Lysates from RK13 cells transfected with WT, S1, S3 and S3.F88W PrP were probed with antibody Sha31, 9A2 and 12B2 after digestion with PNGaseF."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "B, C Additional PrP mutants were examined by Western blot with Sha31 after PNGaseF digestion of RK13 cell lysates. A schematic of the different lengths of C2 caused by single mutations in the OR is shown in the lower portion of (C)."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "D Lysates of N2a, HEK, SMB-PS and SH-SY5Y cells not transfected (−) and transiently transfected with WT PrP or S3 PrP plasmids were digested with PNGaseF and analysed by Western blot using Sha31."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "E Analysis of lysates from RK13 cells transfected with plasmids incorporating Phe substitutions into the hydrophobic domain of WT PrP, S1 PrP and S3 PrP. Lysates were PNGaseF-digested before Western blot using the Sha31 antibody. F1: A114F, F2: G118F, F3: G122F, F4: G126F"
NCBI gene IDs:
"PrP: 19122"
Figure legend: "A Brain homogenates of TgPrP(WT), TgPrP(S1)-17 and TgPrP(S3.F88W)-35 mice were analysed by Western blot before and after PNGaseF digestion using the PrP antibodies Sha31, 12B2 and 9A2. Prnp0/0, and WT brain homogenates were used as controls."
NCBI gene IDs:
"Prnp: 19122
PrP: 19122"
Figure legend: "B The ratio of C2 to full-length PrP in TgPrP(S3.F88W)-35 and WT mice (left panel, n = 7, P = 1.46E-06) and in RK13 cells expressing WT and S3 PrP (right panel, n = 5, P = 4.01E-05) is shown. Values are presented as mean ± SEM. Unpaired, two-tailed t-test, ****P &lt; 0.0001."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "C Different tissue homogenates from a TgPrP(S3.F88W)-35 mouse were PNGaseF-digested and analysed for PrP using the antibody 1A6. FL, full-length PrP; ns, non-specific signal detected in the immunoblotting procedure."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "A Sections of sciatic nerve from TgPrP(S3.F88W)-14 female mice (all 610 days old) and TgPrP(S3.F88W)-35 female mice (614, 570 and 614 days old, respectively) were analysed using toluidine blue staining (upper row), with the third panels for each line showing a hypermyelinated phenotype. Scale bar in left panel represents 50 μm. The lower rows show EM analyses of mice of the indicated genotypes. Scale bar is 10 μm for panels 1-4, with the fifth panel showing a high power view to illustrate hypermyelinated fibres in a mouse expressing S3.F88W PrP (scale bar, 5 μm)."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "B Western blot analysis of sciatic nerve protein extracts (10 μg) from female mice after PNGaseF treatment shows a similar hierarchy of PrP expression in Tg lines to that of brain (see Fig3). The asterisk indicates a greater than full-length fragment present in TgPrP(S3.F88W) mice, possibly corresponding to incomplete removal of the N-terminal signal peptide."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "C Quantification of fibre morphology in aged animals (492-608 days old), representing the percentage of the nerve occupied by fibres versus the total cross-sectional area of the nerve. The S1 and S3.F88W alleles rescue Prnp0/0 nerves to a similar percentage as the WT allele, but the variance and P-value is greater for the two Tg lines expressing the TgPrP(S3.F88W) allele. Unpaired, two-tailed t-test, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001. Sample sizes and exact P-values compared with Prnp0/0 (n = 9) were as follows: TgPrP(WT) n = 6, P = 3.63E-05; Prnp0/0, n = 9; TgPrP(S1)-17 n = 5, P = 3.54E-05; TgPrP(S1)-19 n = 3, P = 6.35E-06; TgPrP(S3.F88W)-14 n = 3, P = 3.70E-03; and TgPrP(S3.F88W)-35 n = 7, P = 7.18E-03."
NCBI gene IDs:
"Prnp: 19122
PrP: 19122"
Figure legend: "Pathology of RML-infected miceA-H The pattern of PrPSc deposition (A-D) and GFAP immunostaining (E-H) is presented.I-P A higher power view of H&amp;amp;amp;E stained hippocampus (I-L) and thalamus (M-P) to reveal vacuolation is presented.Data information: Animals of the same genotype are shown within a column, and incubation times of the presented animals with the RML prion isolate are shown (days). Mice expressing PrP S1 and S3 alleles (central columns) exhibit attenuated PrPSc deposition, gliosis and spongy change. Pathological features of the RML isolate are not altered upon sequential passage through TgPrP(S3.88W)-35 mice (see schematic at bottom of the diagram indicating sequential passages and finally, passage back into WT mice, compare the data in first and fourth columns)."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "A Spot counts in the scrapie cell assay using WT, TgPrP(S1)-17 and TgPrP(S3.F88W)-14 brain homogenates (0.1% or 0.01%) from mice infected with RML are shown in the upper panel (WT vs S1 P = 2.09E-05 and vs S3 P = 4.62E-08) with numbers corrected for PrPC expression levels as per Table1 presented in the lower panel (WT vs S1 P = 1.04E-13 and vs S3 P = 3.39E-20; two-tailed t-test, n = 3)."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "B A comparison of incubation time vs PrPC:PrPSc ratio for mice expressing different levels of WT PrP (WT, TgPrP(WT), tga20), S1 PrP (TgPrP(S1)-17, TgPrP(S1)-19, TgPrP(S1)-39) and S3 PrP (TgPrP(S3.F88W)-14, TgPrP(S3.F88W)-35)."
NCBI gene IDs:
"PrP: 19122"
Figure legend: "A: Relative expression of ELDR in OSCC patient samples compared to adjacent non-tumor tissues (N=20) analyzed by qRT-PCR. Technical triplicates were used from each sample. 18S rRNA was used as an internal control. Data were analyzed by Student's t test. Small bars indicate standard error (*, p&lt;0.05, **, p&lt;0.01; *** p&lt;0.001)."
NCBI gene IDs:
"18S rRNA: 
ELDR: 102725541"
Figure legend: "B: OSCC grade wise upregulation of ELDR expression was noted in the patient samples (N=20). Correlation analysis showed signification positive correlation of ELDR expression with tumor grade (R2= 0.926, significant P value = 0.01). Pearson correlation coefficient and p value was calculated using socscistatistics.com. Well: well differentiated, Mod: moderately differentiated, Poor: poorly differentiated carcinoma. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "C: Representative images of FISH analysis with an RNA probe of ELDR (red) and DAPI staining (blue) in formalin fixed paraffin embedded OSCC tissue. An unrelated probe was used as negative control. Magnification: 40X; inset magnification 100X and scale bar 10 µm."
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "D: Relative expression of ELDR in OSCC cell lines compared to normal oral keratinocytes (NOK) analyzed by qRT-PCR. 18S was used as internal control. Data were analyzed by Student's t test. Small bars indicate standard error (**, p&lt;0.01; *** p&lt;0.001). n = 4 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"18S: 
ELDR: 102725541"
Figure legend: "A: Control or ELDR plasmid DNA stably transfected NOKs were analyzed for cell proliferation using Trypan blue exclusion at the indicated time points. Live cell numbers are presented. n = 4 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "B: Control or ELDR plasmid DNA overexpressed NOK (3×102) were seeded and allowed to form colonies. After two weeks, colonies were stained with crystal violet and counted. Representative images of colonies in control and ELDR overexpressed cells are presented. The right panel shows quantitation. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "C: Cal27, JHU029 and JHU022 cells were transfected with control or ELDR siRNA (50nM), and cell proliferation was analyzed at the indicated time points. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "D: Cell lysates from control or ELDR overexpressing NOKs, control or Cal27 and JHU029 cells treated with two different ELDR siRNAs were subjected to Western blot analysis for PCNA expression using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control for normalization. The bottom panel shows a quantitative representation of Western blot band intensities. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "E: Cal27 and JHU029 cells were transfected with siELDR, ELDR plasmid DNA or both siELDR and ELDR plasmid DNA. Cell lysates were analyzed by Western blot for PCNA expression using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. The right panel shows quantitative representation of Western blot band intensities. n = 2 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "A: Cal27 or JHU029 cells were transfected with control or ELDR siRNA, and EGFR mRNA expression was examined by qRT-PCR. 18S gene was used as internal control. n = 3 biological replicates and 3 technical repeates."
NCBI gene IDs:
"18S: 
EGFR: 1956
ELDR: 102725541"
Figure legend: "B: Cell lysates from control and ELDR depleted Cal27 or JHU029 cells using two different siRNAs to ELDR were subjected to Western blot analysis for the EGFR using specific antibodies. The membrane was reprobed with an antibody against Actin as an internal control. The same actin blot was used in Fig. 2D (for PCNA). The bottom panel shows a quantitative representation of Western blot band intensities. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "C: Cal27 and JHU029 cells were transfected with siELDR, ELDR plasmid or both siELDR and ELDR plasmid. Cell lysates were analyzed by Western blot analysis for EGFR expression using specific antibody. The membrane was reprobed with an antibody against Actin as internal control. The same actin blot was used in Fig. 2E (for PCNA). n = 2 biological replicates."
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "D: Cell lysates from control and ELDR overexpressed NOK were subjected to Western blot analysis for the EGFR using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. The right panel shows a quantitative representation of Western blot band intensities. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "E: Cell lysates from control and ELDR-depleted Cal27 and JHU029 cells were subjected to Western blot analysis for phsospho-STAT3 (pSTAT3 Y-705) and total STAT3 using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. Right panel shows a quantitative representation of Western blot band intensities. n = 2 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "C: Cell lysates from Cal27 and JHU022 cells were incubated with biotinylated ELDR sense and antisense RNA, pulled down and subjected to Western blot analysis for the ILF3 using specific antibodies. Bottom panel shows Western blot analysis of PUM-1 (an unrelated RNA binding protein)."
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "D: Cal27 and JHU022 cell lysates were immunoprecipitated against control or ILF3 antibody and RNA was isolated from the precipitates. Relative expression of ELDR (left penal) and PCAT1 (right panel) was analyzed by qRT-PCR. n = 3 biological replicates and 3 technical replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541
PCAT1: 100750225"
Figure legend: "E: Cell lysates from control and ELDR depleted Cal27 and JHU029 cells were subjected to Western blot analysis for ILF3 using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. Right panel shows the quantitative representation of Western blot band intensities. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "F: Control, ELDR overexpressed and ELDR-depleted Cal27 cells were probed with an antibody against ILF3 (red) and DAPI (blue). Representative confocal microscopic images show nuclear and cytoplasmic expression of ILF3. Arrows indicate cytoplasmic expression. Magnifications 60X, scale bar 20 μ."
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "A: Cal27 and JHU022 cell lysates were immunoprecipitated with control or ILF3 antibodies and RNA was isolated from the precipitates. Relative mRNA expression of EGFR was analyzed by qRT-PCR. n = 3 biological replicates and 3 technical replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "B: Cal27 and JHU029 cells were transfected with control or ILF3 siRNA and after 48hr cell lysates were subjected to Western blot analysis for EGFR and ILF3 using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. n = 3 biological replicates."
NCBI gene IDs:
"ILF3: 3609"
Figure legend: "C: Cal27 and JHU029 cell lysates were transfected with either siELDR or siILF3 and relative mRNA expression of Cyclin E1 was analyzed by qRT-PCR. 18S was used as internal control. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"18S: 
Cyclin E1: 898
ELDR: 102725541
ILF3: 3609"
Figure legend: "D: Control or ELDR depleted Cal27 and JHU029 cell lysates using two different siRNAs to ELDR were subjected to Western blot analysis for Cyclin E1 using specific antibody. The membrane was reprobed with an antibody against Actin as an internal control. Bottom panels show quantitation. n = 4 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "E: Control or ELDR overexpressed NOK lysates were subjected to Western blot analysis for Cyclin E1 using specific antibody. The membrane was reprobed with an antibody against Actin as internal control. Bottom panel shows quantitation. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ELDR: 102725541"
Figure legend: "F: Control or ILF3depleted Cal27 and JHU029 cell lysates were subjected to Western blot analysis for Cyclin E1 using specific antibodies. The membrane was reprobed with an antibody against Actin as internal control. Bottom panels show quantitation. n = 3 biological replicates. Data are represented as the mean ± SD,"
NCBI gene IDs:
"ILF3: 3609"