EMBO/SourceData huggingface dataset:
Figure legend: "A, B. Carfilzomib  and proteasome inhibitor MG132 activate Il-1β luciferase in macrophages. (A) BMDMs were treated with   IL-4 (20 ng/mL) for 24 hours and then stimulated by DMSO, Carfilzomib  (1 μM), or MG132  (5 μM). (B) BMDMs were stimulated by DMSO, Carfilzomib (1 μM) or MG132  (5 μM)) for 1 hour, then induced with  IL-4 (20 ng/mL). Luciferase assays were monitored 12 hours after stimulation. The data are means ± SD of three independent experiments. ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"luciferase: 
Il-1β: 16176"
Figure legend: "E. Carfilzomib, Bortezomib, MLN9708 and MG132 induce ER stress response. BMDMs were pretreated with  IL-4 (20 ng/mL) for 24 hours and then stimulated by  DMSO or  Carfilzomib (1 μM),  Bortezomib  (1 μM), MLN9708  (2 μM), MG132 (5 μM). RNA was extracted from cells 3 hours or 6 hours after stimulation and the expression of ER  stress related genes (Bip, Chop) were detected through  RT-qPCR. The data are means ± SD of three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Chop: 13198
Bip: 14828"
Figure legend: "G. Inhibition of  ER stress impairs the ability of  Carfilzomib, Bortezomib,  MLN9708 and MG132 to reprogram  M2 towards M1-like macrophages. BMDMs were induced by  IL-4 (20 ng/mL) for 24 hours, then pretreated with  4-PBA (5 mM) for 1 hour and stimulated by Carfilzomib  (1μM), Bortezomib (1μM), MLN9708 (2 μM), MG132 (5 μM). RNA was extracted from cells 6 hours after stimulation and the expression of Il-1β,  Il-6 and  Inos were detected through RT-qPCR. The data are means ± SD of three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Il-1β: 16176
Il-6: 16193
Inos: 106736473"
Figure legend: "A, B Deficiency of  Ern1 reduces the expression  of inflammatory related genes. Wild-type and Ern1-/- Raw264.7 cells (A) or BMDMs (B) were pretreated by IL-4  (20 ng/mL) for 24 hours, then stimulated by  DMSO or Carfilzomib  (1 μM). The mRNA of Il-1β and Il-6  were quantified through  RT-qPCR 6 hours after stimulation. The data are means ± SD of three independent experiments. **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Ern1: 78943
Il-1β: 16176
Il-6: 16193"
Figure legend: "E.  Kira6 inhibits the  expression of ER  stress related genes and inflammatory related genes.  BMDMs  was treated by IL-4 (20 ng/mL) for 24 hours, treated with  Kira6  (100 nM) for 1 hour, and then stimulated by  Carfilzomib (1 μM) for 6 hours. RNA was extracted from cells and the expression  of  Bip, Chop,  Il-1β and  Il-6  were quantified through  RT-qPCR. The data are means ± SD of three independent experiments. *p &lt; 0.05, **p &lt; 0.01 (student's t-test). F, G. Carfilzomib has no effect on splicing of XBP1  in M2 macrophages. BMDMs (F) or Raw264.7  cells (G) were pretreated by IL-4 (20 ng/mL) for 24 hours, then stimulated with DMSO, Carfilzomib (1 μM) or TUN  (500 nM). The mRNA expression of sXBP1 and unXBP1 were detected through  RT-qPCR 6 hours after stimulation. The data are means ± SD of three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Chop: 13198
Bip: 14828
Il-1β: 16176
Il-6: 16193
XBP1: 22433"
Figure legend: "L. Carfilzomib activates NF-κB signaling pathway through IRE1α in M2 macrophages. Wild-type and Ern1-/- Raw264.7 cells were pretreated by  IL-4 (20 ng/mL) for 24 hours, then stimulated by  Carfilzomib  (1 μM) for indicated time points. WCL were analyzed by IB  with indicated antibodies."
NCBI gene IDs:
"Ern1: 78943"
Figure legend: "K, L. Carfilzomib shrinks tumor through modulating tumor microenvironment. EG7 cells were inoculated in wild-type or  LyzM-cre;lsl-dTA to allow tumor xenograft  to reach a volume of around 80 mm3. Mice were randomized for treatment (n=5) by saline or Carfilzomib  for two weeks. The  xenografts were dissected to photograph (K) or weigh (L) after treatment. Data are shown as mean ±SD and n indicates the number of biological replicates. *p &lt; 0.05, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"dTA: 
cre: 2777477
LyzM: 17105"
Figure legend: "A. Carfilzomib, Bortezomib and MLN9708 promote the  expression of M1  macrophage markers (Il-1β,  Il-6,  Tnfα) and reduce the expression  of M2 macrophage  markers (Il-10, Tgfβ) in  macrophages  derived from PBMCs. After induced by  IL-4 (20 ng/ml) for 24 hours to differentiate into mature M2 macrophages,  PBMCs derived macrophages  were stimulated by Carfilzomib  (1 μM), Bortezomib (1 μM) or MLN9708 (2 μM). RNA was extracted from cells 6 hours after stimulation and the expression of mRNA were quantified through RT-qPCR. Data from three experiments are presented as the mean ± SD. T test was used for statistical analysis of differences between groups. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Il-10: 3586
Il-1β: 3553
Il-6: 3569
Tgfβ: 7040
Tnfα: 7124"
Figure legend: "(C) Representative FACS plot showing loss of hCD2 marker expression of Ch14 ICL MEFs after treatment with 4-hydroxy tamoxifen (4OHT) for 14 days to induce Cre, compared to Ethanol treated control cells (See also Appendix figure S1A)."
NCBI gene IDs:
"Ch14: "
Figure legend: "(B) Tumor growth curve after early passage ICL cells were injected into flanks of athymic nude mice (n = 5 per group and error bars denote SEM, p&lt;0.05 for Ch9 and Ch14)."
NCBI gene IDs:
"Ch14: 
Ch9: "
Figure legend: "Representative karyotypes of late passage, large T antigen immortalized MEFs after exposure to Cre recombinase and serially sorted for control (hCD2 plus) and ICL (hCD2 Minus) cells for chromosome lines (A) Ch12 Boxed panels denote targeted chromosome loss; arrows and asterisks denote shared and unshared, non-targeted chromosomal copy number variations respectively."
NCBI gene IDs:
"Ch12: "
Figure legend: "Representative karyotypes of late passage, large T antigen immortalized MEFs after exposure to Cre recombinase and serially sorted for control (hCD2 plus) and ICL (hCD2 Minus) cells for chromosome lines (B) Ch14. Boxed panels denote targeted chromosome loss; arrows and asterisks denote shared and unshared, non-targeted chromosomal copy number variations respectively."
NCBI gene IDs:
"Ch14: "
Figure legend: "Representative karyotypes of late passage, large T antigen immortalized MEFs after exposure to Cre recombinase and serially sorted for control (hCD2 plus) and ICL (hCD2 Minus) cells for chromosome lines (C) Ch9 Boxed panels denote targeted chromosome loss; arrows and asterisks denote shared and unshared, non-targeted chromosomal copy number variations respectively."
NCBI gene IDs:
"Ch9: "
Figure legend: "Representative karyotypes of late passage, large T antigen immortalized MEFs after exposure to Cre recombinase and serially sorted for control (hCD2 plus) and ICL (hCD2 Minus) cells for chromosome lines (D) Ch10. Boxed panels denote targeted chromosome loss; arrows and asterisks denote shared and unshared, non-targeted chromosomal copy number variations respectively."
NCBI gene IDs:
"Ch10: "
Figure legend: "Karyotypic analysis of later passage, control and ICL of a replicate (#1) of Ch12 (A). All chromosomes are shown for 20 metaphases. Target chromosomes are boxed in red."
NCBI gene IDs:
"Ch12: "
Figure legend: "Karyotypic analysis of later passage, control and ICL of a replicate (#1) of Ch10 (B) All chromosomes are shown for 20 metaphases. Target chromosomes are boxed in red."
NCBI gene IDs:
"Ch10: "
Figure legend: "Karyotypic analysis of later passage, control and ICL of a replicate (#1) of Ch14 (C) All chromosomes are shown for 20 metaphases. Target chromosomes are boxed in red."
NCBI gene IDs:
"Ch14: "
Figure legend: "Karyotypic analysis of later passage, control and ICL of a replicate (#1) of Ch9 (D) lines. All chromosomes are shown for 20 metaphases. Target chromosomes are boxed in red."
NCBI gene IDs:
"Ch9: "
Figure legend: "(A) Tumor growth curve after later passage ICL cells were injected into flanks of athymic nude mice (n = 5 per group and error bars denote SD, p&lt;0.0005 for Ch9, p&lt;0.05 for Ch10 and p&lt;0.005 for Ch14)."
NCBI gene IDs:
"Ch10: 
Ch14: 
Ch9: "
Figure legend: "(C) Tumor growth curve after later passage ICL cells were injected into flanks of NOD/SCID mice (n = 5 per group and error bars denote SD p&lt;0.0005 for Ch10 and p&lt;0.005 for Ch14)."
NCBI gene IDs:
"Ch10: 
Ch14: 
NOD: 
SCID: "
Figure legend: "(A) Growth curve of the later passage ICL lines Ch10 and Ch14 under adherent in vitro culture conditions (n=3 for each data set and error bars denote SD, p&lt;0.005 for Ch10 and p&lt;0.0001 for Ch14). (See also Appendix Figure S3A)"
NCBI gene IDs:
"Ch10: 
Ch14: "
Figure legend: "(B) Colony formation in ICL and control MEFs. 5000 cells were seeded in 10 cm plates and stained with Methylene blue after three days. Representative colony formation images in Ch10 and Ch14 are shown (left) and quantification of colonies formed (right) in the 4 ICL lines (n=3 for each data set and error bars denote SD, p&lt;0.005 for Ch10, Ch12 and Ch14 and ns for Ch9)."
NCBI gene IDs:
"Ch10: 
Ch12: 
Ch14: 
Ch9: "
Figure legend: "(C) Quantification of growth in ultra-low adherent, sphere forming conditions for the 4 ICL lines (n=3 for Ch10 and Ch14, n=6 for Ch9 and Ch12 and error bars denote SD, p&lt;0.0001 for Ch9, p&lt;0.001 for Ch10 and p&lt;0.05 for Ch12 and Ch14)."
NCBI gene IDs:
"Ch10: 
Ch12: 
Ch14: 
Ch9: "
Figure legend: "(A) Flow cytometry analysis of tail blood from Ch10/+ (control) at 30 days post tamoxifen administration, Ch10/+; Id1 Cre/+ at 30 days post tamoxifen administration, and Ch10/+; Id1Cre/+ at 80 days post tamoxifen administration."
NCBI gene IDs:
"Ch10: 
Id1: 15901"
Figure legend: "(B) Determination of number of γH2AX foci in ICL and control nuclei and quantification (scale bars denote 10μm, p&lt;0.0001 for Ch9, Ch10 and Ch12, p&lt;0.05 for Ch14; n=25 for each dataset and error bars denote SD). (See also Figures S5A and S5B)"
NCBI gene IDs:
"Ch10: 
Ch12: 
Ch14: 
Ch9: "
Figure legend: "(C) Number of γH2AX positive cells in formalin fixed ICL and control tumor sections by immunofluorescence staining and quantification (Scale bars denote 20μm, p&lt;0.0001 for Ch10 and p&lt;0.05 for Ch14)."
NCBI gene IDs:
"Ch10: 
Ch14: "
Figure legend: "(B) Total lysates from LN229 cells in which Merlin was or was not knocked out (KO) were subjected to western blotting."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(E) LN229 cells transduced with a Merlin or scrambled shRNA were treated with DMSO, ionomycin (Iono) or thapsigargin (TG). Total lysates of these cells were subjected to western blotting. The major Merlin band is indicated by an asterisk. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot. Ubiquitinated Merlin is marked with an arrow."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(G) LN229 cells stably transduced with 6x-Histidine-tagged ubiquitin (His-Ubi) were treated with DMSO or thapsigargin (TG). These cells were lysed and subjected to nickel-charged affinity purification followed by western blotting for endogenous Merlin. Both arrowhead and arrow point to the mono-ubiquitinated Merlin. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Ubi: 850620
ubiquitin: 850620"
Figure legend: "(H) LN229 cells stably transduced with 6x-Histidine-tagged ubiquitin (His-Ubi) were detached or reseeded as in (C). These cells were lysed and subjected to nickel-charged affinity purification followed by western blotting for endogenous Merlin. Arrowheads and arrows point to the mono- or di-ubiquitinated Merlin (see a longer exposed blotting results in Figure EV1D). The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Ubi: 850620
ubiquitin: 850620"
Figure legend: "LN229 cells transduced with vector or EGFP-RAC1(Q61L) (D) were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"EGFP: 
RAC1: 5879"
Figure legend: "(E) LN229 cells transduced with vector or Myc-PAK1(T423E) (E) were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Myc: 
PAK1: 5058"
Figure legend: "(F) LN229 cells stably transduced with wild-type Merlin or the indicated mutants were treated with DMSO or thapsigargin (TG) and analyzed by western blot. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot. Native Merlin (asterisk); ubiquitinated Merlin (arrow)."
NCBI gene IDs:
"Merlin: 18016"
Figure legend: "(G) Merlin-depleted LN229 cells stably transduced with Flag-tagged wild-type Merlin or the indicated mutants were treated with DMSO or thapsigargin (TG). The cells were lysed and subjected to immunoprecipitation with a Flag antibody. The lysate and immunoprecipitated products were subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Flag: 
Merlin: 4771"
Figure legend: "(B) LN229 cells transduced with a pool of four siRNAs against NEDD4L (+) or a scrambled siRNA (-) were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane of the short exposure blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(C) LN229 cells stably transduced with indicated shRNAs targeting NEDD4L or a scrambled shRNA were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(D) LN229 cells stably transduced with indicated shRNAs targeting NEDD4L or a scrambled shRNA were detached by trypsinization (see Methods) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(E) LN229 cells stably transduced with 6x-Histidine-tagged Ubiquitin (His-Ubi) were then stably transduced with indicated shRNAs targeting NEDD4L or a scrambled shRNA. These cells were detached as in (D), lysed and subjected to nickel-charged affinity purification followed by western blotting for endogenous Merlin. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"NEDD4L: 23327
Ubi: 850620
Ubiquitin: 850620"
Figure legend: "(A) LN229 cells transduced with a pool of four siRNAs against AMOTL1 (+) or a scrambled siRNA (-) were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"AMOTL1: 154810"
Figure legend: "(B) LN229 cells stably transduced with 6x-Histidine-tagged Ubiquitin (His-Ubi) were then stably transduced with indicated shRNAs against AMOTL1 or a scrambled shRNA control (sh-Co.). These cells were detached, lysed and subjected to nickel-charged affinity purification followed by western blotting for endogenous Merlin. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"AMOTL1: 154810
Ubi: 850620
Ubiquitin: 850620"
Figure legend: "(D) Merlin-depleted LN229 cells stably expressing Flag-HA-tagged Merlin were transduced with scrambled shRNA (sh-Co.) or two distinct shRNAs targeting AMOTL1, treated with DMSO or thapsigargin (TG) and subjected to Flag immunoprecipitation followed by western blotting."
NCBI gene IDs:
"AMOTL1: 154810"
Figure legend: "(F) Merlin-depleted LN229 cells stably transduced with Flag-tagged wild-type Merlin or the indicated mutants were treated with DMSO or thapsigargin (TG). The cells were lysed and subjected to immunoprecipitation with a Flag antibody. The lysate and immunoprecipitated products were subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Flag: 
Merlin: 4771
Merlin: 18016"
Figure legend: "(A) LN229 cells stably transduced with Flag-tagged wild-type Merlin or the S518D mutant were treated with thapsigargin and subjected to immunoprecipitation with a Flag antibody and followed by SDS-PAGE. Outlined gel pieces were analyzed by mass spectrometry. (B) Numbers of unique peptides of Merlin or Ubiquitin from each outlined gel pieces in (A) are shown. "
NCBI gene IDs:
"Flag: 
Merlin: 4771"
Figure legend: "(F) Merlin-depleted LN229 cells expressing His-Ubiquitin were stably transduced with the indicated HA-tagged Merlin forms or an empty vector. These cells treated with DMSO or thapsigargin were subjected to nickel affinity purification in a denaturing step followed by western blotting for HA. Arrows indicate mono-ubiquitinated Merlin. The red asterisk indicates native Merlin and black asterisks indicate non-specific signals. The ratio of mono-ubiquitinated to native Merlin in each lane of the input blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"HA: 
Merlin: 4771
Ubiquitin: 850620"
Figure legend: "(G) Merlin-depleted LN229 cells were stably transduced with an empty vector, Flag-tagged wild-type (WT) Merlin or its K396R mutant. These cells were detached (S, suspension) by trypsinization using the procedure described in the Methods section and reseeded (A, attached) for two hours. Cell lysates were subjected to immunoprecipitation with a Flag antibody followed by western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane of the lysate blot was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Flag: 
Merlin: 4771"
Figure legend: "(H) Merlin-depleted LN229 cells stably transduced with wild-type (WT) Merlin or its K396R mutant were treated with DMSO or thapsigargin (TG) and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(I) Merlin-depleted LN229 cells stably transduced with HA-tagged wild-type (WT) Merlin or its K396R mutant were treated as described in (G) and subjected to western blotting. The ratio of mono-ubiquitinated to native null in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"HA: 
Merlin: 4771"
Figure legend: "(B) Merlin-depleted LN229 cells were reconstituted with wild type (WT) Merlin or its mutants, treated with DMSO or thapsigargin (TG), and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(C) Merlin-depleted LN229 cells were reconstituted with wild type (WT) Merlin or its mutants, treated with DMSO or thapsigargin (TG), and subjected to western blotting. The ratio of mono-ubiquitinated to native Merlin in each lane was quantified by Image J and is shown under the blot."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(D) LN229 cells transfected with a pool of four siRNAs targeting NEDD4L (+) or a scrambled siRNA (-) were treated with DMSO or thapsigargin (TG) and subjected to western blotting."
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(E) Merlin-depleted LN229 cells were stably transduced with empty vector, Flag-tagged wild-type (WT) Merlin, or its K396R mutant. These cells were detached (S, suspension) by trypsinization using the procedure described in Methods and reseeded (A, attached) for two hours. Cell lysates were subjected to western blotting."
NCBI gene IDs:
"Flag: 
Merlin: 4771"
Figure legend: "(F) LN229 cells transfected with a pool of four siRNAs targeting NEDD4L (+) or a scrambled siRNA (-) were detached (Susp., suspension) by trypsinization using the procedure described in Methods and reseeded (Attach, attached) for two hours. Total lysates from these cells were subjected to western blotting."
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(G) LN229 cells transfected with indicated siRNAs were seeded for 3 hours. CTGF or CYR61 mRNA in these cells was quantified by q-RT-PCR. *P&lt;0.05, **P&lt;0.01, ****P&lt;0.0001. Mean±s.e.m, N=4 biological repeats, unpaired t-test."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490"
Figure legend: "(A) LN229 cells stably transduced withHA-Lats1 (two leftmost panels) or vector only (right panel) were treated with DMSO or thapsigargin (TG) and subjected to PLA using HA and Merlin antibodies. Scale bar = 10 μm. (B) PLA signals (dots) in each cell from the results in (A) were quantified. Mean±s.e.m, two-way ANOVA. *P&lt;0.05, ***P&lt;0.001, ****P&lt;0.0001. Each data point represents an image field containing an average of 10 cells. N=4-9 images for each condition as indicated. All images were collected from one experiment. Two independent experiments were performed and gave similar results. "
NCBI gene IDs:
"HA: 
Lats1: 9113"
Figure legend: "(C) Merlin-depleted LN229 cells were stably transduced with HA-tagged wild-type (WT) Merlin or its mutants. These cells were treated with thapsigargin (TG) for 15 minutes and subjected to PLA using HA and Lats1 antibodies. Scale bar = 20 μm. (D) PLA signals (dots) in each cell from the results in (C) were quantified. Mean±s.e.m, Ordinary one-way ANOVA. ****P&lt;0.0001. Each data point represents an image field containing an average of 10 cells. NVector=8, NWT=16, NK396R=18, NK159R=17 images. All images were collected from one experiment. Two independent experiments were performed and gave similar results. "
NCBI gene IDs:
"HA: 
Merlin: 4771"
Figure legend: "(E) Merlin-depleted LN229 cells stably transduced with empty vector, Flag-tagged wild-type (WT) Merlin, or its K396R mutant were subjected to immunoprecipitation with a Flag antibody followed by western blotting."
NCBI gene IDs:
"Flag: 
Merlin: 4771"
Figure legend: "(G) Merlin-depleted LN229 cells were stably transduced by HA-tagged Merlin. These cells were then transduced with a pool of four siRNAs against NEDD4L or a scrambled control siRNA and treated with DMSO or thapsigargin (TG). PLA was performed in these cells using HA and Lats1 antibodies, and the PLA signals (dots, showed in Figure EV5E) in each cell were quantified. Mean±s.e.m, two-way ANOVA. ****P&lt;0.0001. Each data point represents an image field containing averagely 10 cells. N=12 images in all conditions, except for Nsi-Control:TG=13 images. All images were collected from one experiment. Two independent experiments were performed and showed similar results."
NCBI gene IDs:
"HA: 
NEDD4L: 23327
Merlin: 4771"
Figure legend: "(A) Met5-A cells stably transduced with the indicated shRNAs targeting Merlin or a scrambled shRNA were subjected to the BrdU incorporation assay. Scale bar = 40 μm. (B) The percentage of BrdU positive cells from (A) was quantified. Mean±s.e.m, N=3 biological repeats, paired t-test. **P&lt;0.01, ***P&lt;0.001."
NCBI gene IDs:
"Merlin: 4771"
Figure legend: "(C) Met5-A cells stably transduced with the indicated shRNAs targeting NEDD4L or a scrambled shRNA were subjected to the BrdU incorporation assay. Scale bar = 40 μm. (D) The percentage of BrdU positive cells from (C) was quantified. Mean±s.e.m, N=3 biological repeats, paired t-test. **P&lt;0.01, ***P&lt;0.001. "
NCBI gene IDs:
"NEDD4L: 23327"
Figure legend: "(E) Meso-33 cells transduced with vector or the indicated Merlin forms were subjected to the BrdU incorporation assay. Scale bar = 40 μm. (F) The percentage of BrdU positive cells from (E) was quantified. Mean±s.e.m, N=3 biological repeats, ordinary one-way ANOVA. *P&lt;0.05, **P&lt;0.01. "
NCBI gene IDs:
"Merlin: 4771"