EMBO/SourceData huggingface dataset:
Figure legend: "(C) Cell proliferation assay of adenoma-derived non-cancerous SB and cancerous 10C and human CRC cell lines HCT116, DLD1, SW480, HT29 and RKO either untreated (black lines) or following treatment with control siRNA (gray lines) or ANXA1-specific siRNA (green lines). Data are mean values ± SEM. The experiment was performed in triplicates and repeated three times. *p&lt;0.05, ** p&lt;0.01 and *** p&lt;0.001, compared to the untreated cells (two-way ANOVA)."
NCBI gene IDs:
"ANXA1: 301"
Figure legend: "(D) Cell proliferation assay of SB (left panel) and RKO (right panel) cells transfected with ANXA1 (red line), as compared to the control cells (black lines). Data are mean values ± SEM. The experiment was performed in triplicates and repeated three times. *p&lt;0.05, **p&lt;0.01 and ***p&lt;0.001 (two-way ANOVA)."
NCBI gene IDs:
"ANXA1: 301"
Figure legend: "(E) Xenografted tumor growth in nude mice following subcutaneous and bilateral inoculation of HCT116 cells transfected with control siRNA or ANXA1-specific siRNA. The tumor volumes were measured after 7 days post injection (left panel, n=4). For each mouse, the tumor resulting from ANXA1-specific siRNA treated cells was normalized to that from control siRNA-treated cells, which was designated 100%. The line represents the average. *p&lt;0.05 (paired t-test). The individual tumor pairs are shown on the right panel: top, tumors arising from control siRNA treated cells; bottom, tumors arising from ANXA1-specific siRNA treated cells."
NCBI gene IDs:
"ANXA1: 301"
Figure legend: "(A) Real-time qPCR analysis of ANXA1 mRNA levels in SB, 10C, HCT116 and DLD1 cells incubated with wild-type F. nucleatum 12230 (Fn) or fadA-deletion mutant US1 (US1) at MOI of 50:1 for the indicated time periods. The results were normalized to those obtained with untreated cells and were the mean of 3 independent experiments each performed in triplicates. *p&lt;0.05, **p&lt;0.01 and ***p&lt;0.001 (two-way ANOVA)."
NCBI gene IDs:
"ANXA1: 301
fadA: 31730396"
Figure legend: "(C) Flow cytometry analysis of Annexin A1 in 10C cells transfected with control siRNA (dotted black line) or CDH1-specific siRNA (dotted red line) followed by no treatment, or incubation with BSA (1000 µg/ml) or FadAc (1000 µg/ml) for 1 hr. C, untreated control. Data are mean values ± SD. The experiment was performed in triplicates and repeated twice. ***p&lt;0.001 (two-way ANOVA)."
NCBI gene IDs:
"CDH1: 999"
Figure legend: "Flow cytometry analysis of SB (A), 10C (B), DLD1 (C) and HCT116 (D) cells incubated with CFSE-labeled F. nucleatum 12230 (Fn) or its fadA-deletion mutant US1 (US1) at MOI of 10-20:1 for the indicated time and immuno-stained with anti-Annexin A1 antibodies. Shown on the top panels are the density plots. x-axis, CFSE-labeled F. nucleatum or US1 (CFSE-Fn); y-axis, Annexin A1 (ANXA1). Shown on the bottom panels are the percentages of Annexin A1-positive (solid bars) or negative (clear bars) cells bound by F. nucleatum or US1 out of the total number of cells analyzed. Data are mean values ± SD. The experiments were performed in triplicates and repeated 2-3 times. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001 (two-way ANOVA)."
NCBI gene IDs:
"fadA: 31730396"
Figure legend: "(A) Colorectal tumors generated in Apcmin/+ mice following treatment with PBS, E. coli DH5α (E. coli), fadA-deletion mutant US1 (US1) or F. nucleatum 12230 (Fn). Each symbol represents one mouse. Horizontal lines represent mean values. Representative tumors formed in the mouse colon are shown on the right, pointed by blue arrows on the right. *p&lt;0.05, **p&lt;0.01 (one-way ANOVA)."
NCBI gene IDs:
"Apc: 11789
fadA: 31730396"
Figure legend: "(B) ANXA1 mRNA levels in Apcmin/+ mouse colonic tumors and normal colonic tissues as measured by real-time qPCR. Each symbol represents one mouse. Horizontal lines represent mean values. *p&lt;0.05, **p&lt;0.01 (two-way ANOVA)."
NCBI gene IDs:
"ANXA1: 301
Apc: 11789"
Figure legend: "(C) Positive correlation between fadA gene copy numbers (x-axis) and ANXA1 mRNA levels (y- axis) in F. nucleatum-induced Apcmin/+ mouse colonic tumors (n=34; Pearson's correlation). Each dot represents the average of qPCR results performed in duplicates"
NCBI gene IDs:
"ANXA1: 301
Apc: 11789
fadA: 31730396"
Figure legend: "(D) Abundance of fadA in the paired normal and adenocarcinoma tissues from human CRC patients (n=18) expressed as ratio of fadA over total 16S rRNA genes determined by qPCR. Each symbol shows the average of duplicate qPCR results from one patient. Horizontal lines represent mean values. **p&lt;0.01 (paired t-test)."
NCBI gene IDs:
"16S rRNA: 
fadA: 31730396"
Figure legend: "(E) ANXA1 mRNA levels in paired normal and adenocarcinoma tissues from CRC patients (n=18). Each symbol shows the average of duplicate qPCR results from one patient. Horizontal lines represent mean values. **p&lt;0.01 (paired t-test)."
NCBI gene IDs:
"ANXA1: 301"
Figure legend: "(F) Positive correlation between fadA gene copy numbers (x-axis) and ANXA1 mRNA levels (y-axis) in human colorectal adenocarcinoma tissues (n=18; Pearson's correlation). Each dot represents the average of qPCR results performed in duplicates."
NCBI gene IDs:
"ANXA1: 301
fadA: 31730396"
Figure legend: "(A) Flow cytometry analysis of β-catenin expression in 10C, HCT116 and DLD1 cells following transfection with control siRNA (clear bars) or ANXA1-specific siRNA (solid bars) and incubation with F. nucleatum 12230 at MOI of ~20:1 for indicated time periods. The geometric means of cells treated with control siRNA at time 0 was designated as 1. Data are mean values ± SD. The experiment was performed in duplicates or triplicates and repeated 1-3 times. **p&lt;0.01, *** p&lt;0.001 (two-way ANOVA)."
NCBI gene IDs:
"ANXA1: 301
β-catenin: 1499"
Figure legend: "(B) Immuno-staining of β-catenin in 10C cells following transfection with control siRNA or ANXA1-specific siRNA and incubation with F. nucleatum 12230 (Fn) at MOI of ~100:1 for 2 hrs. β-catenin was stained with Alexa Fluor®680 (red) and the nuclei with DAPI (blue). The images were captured with confocal microscope at 800x magnification. -, no bacteria added. Note the increased expression of β-catenin and its nucleus translocation in response to F. nucleatum in control siRNA-treated cells, but not in ANXA1 siRNA-treated cells. The experiment was repeated twice. Scale bar, 200 nm."
NCBI gene IDs:
"ANXA1: 301"
Figure legend: "(C) Real-time qPCR analysis of CCND1 expression using mRNA extracted from the cancerous 10C, and human CRC cells HCT116 and RKO, each grown to 50% or 100% confluency. Data are mean values ± SEM. The experiment was performed in triplicates and repeated twice. *p&lt;0.05, ***p&lt;0.001 (student's t-test)."
NCBI gene IDs:
"CCND1: 595"
Figure legend: "(D) Western-blot analysis of Cyclin D1, Annexin A1, and β-actin in RKO cells transfected with control vector or ANXA1. Induction of Cyclin D1 was observed in response to increased Annexin A1. The experiment was repeated twice."
NCBI gene IDs:
"ANXA1: "
Figure legend: "(F) Movements of KIF1A-EGFP and KIF1A(E239K)-EGFP expressed in KIF1A knockdown DRG and the scrambled control. White scale bar, 1 μm. (G) Anterograde velocities of the indicated genotypes calculated from the kymograph slopes. Data are shown as mean and SD. KD+KIF1A-EGFP, n = 29; KD+KIF1A(E239K)-EGFP, n = 32; scrambled+KIF1A-EGFP, n = 44; scrambled+KIF1A(E239K)-EGFP, n = 42 from three independent cultures. ns, p = 0.3329; **, p &lt; 0.01; ****, p &lt; 0.0001; Unpaired t test."
NCBI gene IDs:
"EGFP: 
KIF1A: 16560"
Figure legend: "(F) Rate constants (kobs) determined for the reaction of mant-ATP with nucleotide-free KIF1A(WT) and KIF1A(E239K) were plotted against concentration of mant-ATP. Data are represented as the mean ± SD. The data from three independent measurements were analysed."
NCBI gene IDs:
"KIF1A: 16560"
Figure legend: "(A) Western blot of Beclin‐1 and FACS profiles following siRNA treatment in HeLa cells and cells stably expressing a HA‐Beclin‐1 construct resistant to the siRNA targeting endogenous Beclin‐1."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(C,D) Quantification of cells in mitosis (C) and quantification of each step of mitosis (D) by H3Ser10P staining for Control and Beclin‐1 siRNA‐treated cells. Error bars represent standard deviations (s.d.) (n=number of counted cells) calculated on three independent experiments. Two and three asterisks indicate significant results (P=0.0028 and P=0.0006, respectively)."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(E) Knockdown of Beclin‐1 increases the number of mitotic cells with misaligned chromosomes. siRNA‐treated HeLa cells were fixed in PFA, immunolabelled for α‐tubulin (green) and stained with DAPI (blue). White arrows indicate misaligned chromosomes. Quantification of cells with misaligned chromosomes is shown at the right (n=number of counted cells). Three asterisks indicate significant results (P=0.0001). Scale bar, 10 μm. DAPI, 4′,6‐diamidino‐2‐phenylindole; HA, haemagglutinin; PFA, paraformaldehyde; siRNA, small interfering RNA."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "Beclin‐1 depletion prolongs prometaphase by delaying chromosome congression. (A-H) Time‐lapse films of HeLa cells stably expressing YFP‐α‐tubulin and H2B‐Cherry and treated with the indicated siRNA. The pictures are acquired every 10 min. Time lapse starts at nuclear envelope breakdown. (A) Mitosis in control cells. (B) Western blot of whole‐cell lysates from Control, Beclin‐1 and Beclin‐1+Mad2 siRNA‐treated cells. (C-E) Three different classes of mitotic progression were observed upon Beclin‐1 depletion. Higher magnification of lagging chromatids in the anaphase of Beclin‐1‐depleted cells is shown on the right of (D)."
NCBI gene IDs:
"Beclin‐1: 8678
Mad2: 10459///4085"
Figure legend: "(F) The prometaphase delay is dependent on MAD2 and the spindle checkpoint. Cells treated simultaneously with siRNA targeting MAD2 and Beclin‐1 rapidly exit mitosis. (G) Average duration of mitosis in different classes of Beclin‐1‐depleted cells, compared to control and MAD2 depletions. n=number of counted cells in three independent experiments. Error bars on graphs represent s.d."
NCBI gene IDs:
"Beclin‐1: 8678
MAD2: 10459///4085"
Figure legend: "(H) The frequency of the different Beclin‐1 phenotypes depended on the efficiency of Beclin‐1 knockdown. Mitotic progression in cells treated with increasing concentrations of Beclin‐1 siRNA was followed by video‐microscopy. Left panel: western blot of Beclin‐1 upon RNAi treatment; right panel: quantification of the appearance of the three different mitotic progression patterns, that is, prolonged mitosis with normal anaphase (as C), with lagging chromatids (as D) and mitotic exit without clear anaphase (as E) upon increased Beclin‐1 knockdown experiments. NEB, nuclear envelop breakdown; RNAi, RNA interference; siRNA, small interfering RNA; YFP, yellow fluorescent protein."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(A) Inter‐kinetochore distance is reduced in unaligned chromosomes of Beclin‐1‐depleted cells. The distance between each kinetochore pair was quantified in siRNA‐treated and control cells treated with nocodazole for 3 h before fixation. Cells were stained with anti‐CREST antibody and DAPI. Quantification of the distance between kinetochore pairs (indicated by coloured bars) is reported at the bottom and done on sister kinetochores present in the same focal plane. One asterisk indicates significant result (P=0.0365). Graphs represent three independent experiments (error bars=s.d.)."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(B) Depletion of Beclin‐1 leads to defects in K‐MT attachment. siRNA‐treated cells were cooled on ice before fixation to reveal stable k‐fibres and then stained with anti‐α‐tubulin (red) and anti‐CREST (green) antibodies. Numbers point to magnified areas and indicate the mode of attachment of k‐fibres to kinetochores (1, bi‐oriented; 2, unattached; 3, mono‐oriented kinetochores). Scale bar, 10 μm. DAPI, 4′,6‐diamidino‐2‐phenylindole; NS, not significant; siRNA, small interfering RNA."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(G) Western blot of whole‐cell lysates from control and Beclin‐1 siRNA‐treated cells. siRNA, small interfering RNA."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(C) Zwint‐1 levels do not change in Beclin‐1‐depleted cells. Cells were treated with nocodazole before fixation. On the right, magnified individual kinetochore pairs stained for Zwint‐1 (red) and CREST (green), and quantification of Zwint‐1 levels normalized to CREST signal from three independent experiments (error bars=s.d.)."
NCBI gene IDs:
"Beclin‐1: 8678"
Figure legend: "(H) Beclin‐1 localization close to the kinetochore does not depend on the ZW10 protein. Cells treated with Control, Beclin‐1 and ZW10 siRNA were pre‐extracted and fixed before labelling with Beclin‐1 (green), CREST (blue) and ZW10 (red) antibodies. DNA was visualized with DAPI. (C-H) Scale bar, 10 μm. DAPI, 4′,6‐diamidino‐2‐phenylindole; DNA, deoxyribonucleic acid; GFP, green fluorescent protein; GST, glutathione S‐transferase; HA, haemagglutinin."
NCBI gene IDs:
"Beclin‐1: 8678
ZW10: 9183"
Figure legend: "F.  Proliferation  curves of factor-independent TF-1 TpoR CALRdel61 cells cultured with 2, 10 or 20 µg/mL   4D7 or 20 µg/mL of control IgG antibody (n=3 biological replicates). G Proliferation curves of factor-independent TF-1 TpoR CALRdel52 cells cultured with 10 or 20 µg/mL  4D7  or 20 µg/mL of control IgG antibody (n=3 biological replicates). Data information: bars represent standard error of the mean summarizing 3 biological replicates performed in triplicate, with a one-way ANOVA with Bonferroni correction used to determine statistical significance."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "B. Similar experiment using TPO-independent TF-1 TpoR CALRdel61 cells. C. Similar experiment using TPO-independent  TF-1 TpoR CALRdel52 cells at 8 hours. D. Similar experiment using PBMNCs from CALRdel52 PMF  primary cells at 8 hours. Additionally, cells were treated with 280 nM of ruxolitinib as a positive control."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "F. Western blot showing caspase 3  cleavage occurring within 24 hours of TPO  withdrawal in TPO  dependent  TF-1 TpoR cells. An increase in cleaved caspase 3 is observed after 48 hours of treatment with 20 µg/mL  4D7 in TF-1 TpoR CALRdel61 and TF-1 TpoR CALRdel52 cells."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "G. Western blot of  TpoR immunoprecipitation under non-reducing conditions showing associated CALR  50 kDa monomers and 100 kDa dimers (red arrowheads) present only in TF-1 TpoR CALRdel61 disrupted by 8-hour treatment with 20 µg/mL 4D7 but not  PBS  or 20 µg/mL IgG. CALR  monomers &amp; dimers are detectable by polyclonal anti-wild type CALR or anti-mutant CALR  monoclonal antibodies. Red arrowheads, detected mutant CALR  protein; brown arrowheads, detected wildtype CALR protein, asterisk, non-specific bands."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "B. Graph showing the decreased fold change of CD41+CD61+  megakaryocytes cultured in  4D7  compared to IgG. FACS-sorted CD34+ from myelofibrosis patients with  CALR  or JAK2  mutation were cultured over 12 days without TPO in the presence of  SCF, IL-6  and IL-9. Black columns show JAK2V617F mutation positive samples (n= 4 technical replicates of samples from different patients). Data information: Unpaired students t-test used to determine statistical significance Bars represent standard deviations *, P = 0.05 - 0.01, **, P = 0.01 - 0.001, ***, P = 0.001 - 0.0001, ****, P &lt;0.0001, n.s, not significant."
NCBI gene IDs:
"CALR: 811
JAK2: 3717"
Figure legend: "C. Number of CD41+ megakaryocytes  derived from isolated  CD34+ progenitors from myelofibrosis patients. Number of  CD41/CD61+ cells counted on day 12 using  trypan blue  exclusion (n=1 technical replicate). D. Summary of fold change reduction of  CD41/CD61+ megakaryocytes by 4D7 in all tested CALR  mutated patient  samples compared to CALR wild type, normalised to IgG (n=11 samples from different patients, with 4 technical replicates per sample). Data information: Bars represent standard error of means Unpaired students t-test used to determine statistical significance *, P = 0.05 - 0.01, **, P = 0.01 - 0.001, ***, P = 0.001 - 0.0001, ****, P &lt;0.0001, n.s, not significant."
NCBI gene IDs:
"CALR: 811"
Figure legend: "E Number of  CD41+ megakaryocyte  colonies from patient  samples after  4D7  treatment. CD34+ from  patients with myelofibrosis  with  CALRdel52 or CALRins5 were plated on  collagen-based matrix in presence of 20 µg/mL  4D7  or IgG control (n=3 biological replicates). Data information: Unpaired students t-test used to determine statistical significance Bars represent standard deviations *, P = 0.05 - 0.01, **, P = 0.01 - 0.001, ***, P = 0.001 - 0.0001, ****, P &lt;0.0001, n.s, not significant."
NCBI gene IDs:
"CALR: 811"
Figure legend: "F. Representative micrographs showing CD41+ colonies in pink and CD41- colonies in blue after treatment with  4D7 or IgG  at 100 X or 40X magnification. Scale bar indicates 100µm. G. Summary of fold change reduction of CD41+  megakaryocytes in CALR mutated samples cultured MegaCult treated with 4D7  compared to IgG (n= 4 biological replicates). Data information: Bars represent standard error of means Unpaired students t-test used to determine statistical significance *, P = 0.05 - 0.01, **, P = 0.01 - 0.001, ***, P = 0.001 - 0.0001, ****, P &lt;0.0001, n.s, not significant."
NCBI gene IDs:
"CALR: 811"
Figure legend: "H. Number of  megakaryocyte colony forming units grouped according to colony cell number after  4D7   treatment in a mutant CALRins5 sample (n=3 biological replicates). Data information: Unpaired students t-test used to determine statistical significance Bars represent standard deviations *, P = 0.05 - 0.01, **, P = 0.01 - 0.001, ***, P = 0.001 - 0.0001, ****, P &lt;0.0001, n.s, not significant."
NCBI gene IDs:
"CALR: 811"
Figure legend: "F. Western blot  showing signalling in ruxolitinib-resistant TF-1 TpoR CALRdel61 compared to ruxolitinib sensitive TF-1 TpoR CALRdel61 cells after treatment with 100nM  ruxolitinib for 16 hours or 30 minutes and blotted for phospho-STAT5, total STAT5, phospho-ERK, total ERK and  actin  as indicated. Ruxolitinib-sensitive  cells were non-viable after 16 hours of treatment."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "G. Comparison of  cell growth after DMSO, 20 µg/mL   4D7, IgG or 100 nM ruxolitinib  treatment over 4 days of ruxolitinib-resistant  TF-1 TpoR CALRdel61. Cells counted using Trypan Blue  exclusion (n=3 biological replicates with 3 technical replicates). H. Number of colonies observed from cells plated in MethoCult following 72 hours of treatment with DMSO, 20 µg/mL  4D7, IgG or 100nM ruxolitinib performed in ruxolitinib-resistant TF-1 TpoR CALRdel61 cells (n=3 biological replicates with 3 technical replicates). Data information: Error bars on represent standard deviation. All P values are unpaired Student's unpaired t-test."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "D. Kaplan-Meier survival  curve of bone marrow engraftment  model of TF-1 TpoR CALRdel61 cells showing improved survival of mice treated with 4D7   compared with IgG control commencing 1 week after tail vein injection (n=5 mice per treatment). Data information: For all survival  curves the log-rank Mantel-Cox test P value is shown."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "F. Kaplan-Meier survival curve of TF-1 TpoR CALRdel61 chloroma mice  treated with  4D7 or IgG until humane killing due to tumour diameter &gt; 30 mm or ulceration. (n=3  mice  per treatment) G. Kaplan-Meier survival curve of NSG mice engrafted  with  ruxolitinib-resistant TF-1 TpoR CALRdel61 treated with 12.5 mg/kg  4D7  or IgG twice weekly (n=5 and 6 mice  for IgG and   4D7 respectively). Data information For all  survival curves  the log-rank Mantel-Cox test P value is shown."
NCBI gene IDs:
"CALR: 811
TpoR: 4352"
Figure legend: "DRiP labelling in GFP-NCL HeLa Kyoto cells that were either left untreated or treated with OP-puro (25 µM) for 2 h. Where indicated cells were allowed to recover in drug-free medium (control) or in presence of MG132 (10 µM) for 5 h. Scale bars: 5 µm. GFP-NCL HeLa Kyoto cells were treated as described in A, with the exception that a lower concentration of OP-puro was used (5 µM). The distribution of DRiPs and GFP-NCL was analyzed by STED super-resolution microscopy. Scale bars: 5 µm."
NCBI gene IDs:
"GFP: 
NCL: 4691"
Figure legend: "DRiP labelling in GFP-NCL HeLa Kyoto cells that were either left untreated or treated with HS at 42˚C and OP-puro (25 µM) for 2 h. Where indicated, cells were cotreated with CHX (50 μg/ml)."
NCBI gene IDs:
"GFP: 
NCL: 4691"
Figure legend: "HSPA8 subcellular distribution and recruitment inside nucleoli following exposure to HS at 42˚C for 2 h, alone or combined with CHX, in GFP-NCL HeLa Kyoto cells."
NCBI gene IDs:
"GFP: 
NCL: 4691"
Figure legend: "HeLa cells were transfected with vectors encoding for GFP, GFP-GR50 or RPL23a-GFP. 24 hrs post-transfection cells were fixed and stained with Amylo-glo. The percentage of transfected cells with Amylo-Glo positive nucleoli is shown. Number of cells counted/condition: GFP, 412; RPL23a-GFP, 482; GFP-GR50, 359, in three independent experiments; statistical significance via One-way ANOVA; p &lt; 10-7, ± s.e.m."
NCBI gene IDs:
"GFP: 
GR50: 
RPL23a: 6147"
Figure legend: "DRiPs, polyUb proteins (FK1) and PML labelling in HeLa cells treated with OP-puro (25 µM) and heat shock (HS) at 42˚C for 2 h; where indicated cycloheximide was also added (CHX; 50 μg/ml). Scale bars: 5 µm. Lower panel: STED super-resolution microscopy showing colocalization of DRiPs with PML-NBs in PML-GFP HeLa cells treated with a low concentration of OP-puro (5 µM) and heat shock (HS) at 42˚C for 2 h. PML-NBs were visualized using a PML specific antibody, followed by incubation with alexa fluor 647. For convenience the alexa fluor 647 signal is shown in green. Scale bars: 5 µm."
NCBI gene IDs:
"GFP: 
PML: 5371"
Figure legend: "STED super-resolution microscopy showing colocalization of endogenous polyUb proteins (FK1) and PML-NBs in PML-GFP HeLa cells treated with HS at 42˚C for 2 h. Scale bars: 5 µm."
NCBI gene IDs:
"GFP: 
PML: 5371"
Figure legend: "DRiPs, polyUb proteins (FK1) and PML labelling in HeLa cells treated with MG132 (10 µM) and OP-puro (25 µM) for 4 h; where indicated, cycloheximide was also added (CHX; 50 μg/ml). Asterisks (*) indicate nucleoli. Scale bars: 10 µm. Lower panel: STED super-resolution microscopy showing colocalization of DRiPs with PML-NBs in PML-GFP HeLa cells treated with a low concentration of OP-puro (5 µM) and MG132 (10 µM) for 4 hrs. Scale bars: 5 µm. Quantitation of cells shown in E. Number of cells counted/condition: 298 - 1713 in three independent experiments; statistical significance via One-way ANOVA; p ≤ 0.001, ± s.e.m."
NCBI gene IDs:
"GFP: 
PML: 5371"
Figure legend: "HeLa cells were lipofected with non-targeting siRNA control or a specific siRNA against PML. 72 h post-transfection, total proteins were extracted and expression levels of PML were analyzed by immunoblotting. TUBA4A was used as loading control."
NCBI gene IDs:
"PML: 5371"
Figure legend: "HeLa cells were lipofected with non-targeting siRNA control or a specific siRNA against PML. 72 h post-transfection, cells were treated with MG132 (10 µM) and OP-puro (25 µM) for 4 h. Left panel: automated quantitation of the number of PML-NBs/nucleus is reported. Statistical significance via 2‐tailed Student's t‐test. Cell number analyzed: 334 - 364; p &lt; 10-10. Right panel: automated quantitation of the number of DRiP foci/nucleus. Cell number analyzed: 334 - 364; p &lt; 10-10. Automated DRiP segmentation is based on azide signal."
NCBI gene IDs:
"PML: 5371"