EMBO/SourceData huggingface dataset:
Figure legend: "(E) Forcing the interaction of Smaug with activated FU leads to a SMO-independent phosphorylation of Smaug. Mean values of the percentage of phosphorylated Smaug in Cl8 cells coexpressing λN-SNAP-Smaug with GFP-FU, FU-SBR, FUEE-SBR or FUDANA-SBR, with or without HH, as indicated. N=3 (biological triplicates). See also Fig EV4C and D. Note that the levels of Smaug phosphorylation observed in presence of FUDANA-SBR are not lower than those seen with GFP-FU. This suggests an incomplete inhibition of endogenous FU, which may be due to the trapping of FUDANA-SBR by Smaug. See a representative blot in Fig EV3C and a phosphatase assay in Fig EV4D."
NCBI gene IDs:
"GFP: 
SNAP: 
FU: 32855
λN: 2703540
Smaug: 39034"
Figure legend: "(A and C) Effect of FUEE-SBR on Smaug phosphorylation and levels. Extracts of Cl8 cells transfected with λN-SNAP-smaug (25 ng) and SNAP-GUS-5BoxB (300 ng) plasmids in absence or with different amounts of FUEE expressing plasmid (ranging from 15 to 60 μg, as indicated) were analyzed by electrophoresis before quantification as above. See a reprenstive gel in Fig EV5A. The % of phosphorylated SNAP-Smaug protein (determined as in Fig 3) (A) are represented as a function of the levels of FUEE-SBR. The mean values and (SD) for independent biological triplicates (N=3) are shown in the graph at the bottom. The statistical analysis was done by one tailed bivariate Wilcoxon rank test with a p value= 0.05 (*). Here CLIP-GUS was used as an internal control."
NCBI gene IDs:
"5BoxB: 
GUS: 
SNAP: 
FU: 32855
λN: 2703540
smaug: 39034"
Figure legend: "(B) Effect of FUEE-SBR on Smaug activity. The relative levels of reporter expression obtained in the same experiment as in (A-C) are represented as a function of the % of phosphorylated Smaug. Color of data points correspond to ng of transfected FUEE-SBR in A-C."
NCBI gene IDs:
"FU: 32855"
Figure legend: "(A and C) Effect of FUEE-SBR on Smaug phosphorylation and levels. Extracts of Cl8 cells transfected with λN-SNAP-smaug (25 ng) and SNAP-GUS-5BoxB (300 ng) plasmids in absence or with different amounts of FUEE expressing plasmid (ranging from 15 to 60 μg, as indicated) were analyzed by electrophoresis before quantification as above. See a reprenstive gel in Fig EV5A. The relative levels of of λN-SNAP-Smaug protein (C) (determined as in Fig 2) are represented as a function of the levels of FUEE-SBR. The mean values and (SD) for independent biological triplicates (N=3) are shown in the graph at the bottom. The statistical analysis was done by one tailed bivariate Wilcoxon rank test with a p value= 0.05 (*). Here CLIP-GUS was used as an internal control."
NCBI gene IDs:
"5BoxB: 
GUS: 
SNAP: 
FU: 32855
λN: 2703540
smaug: 39034"
Figure legend: "(D) Effect of FUEE-SBR on Smaug subcellular localization.Representative fluorescent images of Cl8 cells expressing GFP-SmaugWT (1), FUEE-SBR-mCherry (2), FUDANA-SBR-mCh (3) or expressing GFP-SmaugWT together with FUEE-SBR-mCherry (4-4'') or GFP-SmaugWT together with FUDANA-SBR-mCh (5-5''). The merge images in 4&quot; and 5&quot; show GFP-SmaugWT in green and FUEE-SBR-mCh or FUDANA-SBR-mCh in red. (6) In presence of FUEE-SBR, 274 of a total of 294 cotransfected cells show a diffuse localization of Smaug and FU and no foci. In presence of FUDANA-SBR, 100% of the cotransfected cells (n=241) show Smaug and FU distributed in bodies and colocalized. 250 ng of plasmid were used for each construct. Scale bar (shown in D1, identical for all panels): 10μ"
NCBI gene IDs:
"GFP: 
mCh: 
mCherry: 
FU: 32855
Smaug: 39034"
Figure legend: "(A) Effect of FUEE-SBR and HH/SMO on endogeneous Smaug target mRNAs in Cl8 cells. Relative mRNA levels estimated using semi-quantitative RT-PCR for various Smaug's targets in Cl8 cells expressing FUEE-SBR (black) , FUEE (dark grey) , or FUDANA-SBR (light grey) were reported with respect to levels of a GFP control. mRNA levels were set to 1 for the GFP control. The list of genes and the sequences of the primers used are shown in Appendix Table S2 and S3. See also Fig EV5E. Here and in Fig EV5D: RNA encoding mitochondrial proteins are written in blue. N=3 (biological triplicates) and the quantifications were repeated 3 times on each sample.The results are presented as the means ± standard error. The differences between groups were assessed using Student's t-tests with Prism V8.2.1 software. A p value &lt;0.05 was considered to indicate a statistically significant difference"
NCBI gene IDs:
"FU: 32855
SMO: 33196"
Figure legend: "(B) Effect of HH on endogeneous targets of Smaug in the wing imaginal disc. Relative levels of Smaug's targets mRNAs in MS1096; UAS-hh or hhts and their respective controls (as in Fig EV5X). N=3, with xx disc for each phenotype."
NCBI gene IDs:
"MS1096: 
hh: 42737
UAS: 5658215///5658214
Smaug: 39034"
Figure legend: "(C) Effect of smaug mutation on [fu1] wing class distribution. Percent distribution of phenotypic classes (defined in Appendix Figure S4) in fu1 males in presence of zero (fu1), one (fu1;;smaug47/+) or two copies (fu1;; smaug47/smaug47) of the smaug47 allele. The wings were double-blindly classified in &quot;weak&quot; and &quot;strong&quot; according to the LV3-V4 defects (see Fig EV6B). *Chi2 statistical analysis gave a p of 0,015 for fu1 and fu1;;smaug47/+ distributions and of 0,0024 when comparing fu1;;smaug47/+ to fu1;; smaug47/smaug47."
NCBI gene IDs:
"fu: 32855
smaug: 39034"
Figure legend: "Flow cytometry analysis of PD-L1 after gemcitabine and RT (as described above) in KPC (E) and Pan02 (F) following Stat1 downregulation by siRNA (mean ± SD, n=3, Student's t-test). MFI, mean fluorescence index;"
NCBI gene IDs:
"Stat1: 20846"
Figure legend: "B. Representative two-dimensional IFT-tracks from WT, kinesin-II (klp-11), and osm-3 null animals. Red and violet circles indicate the start and end of the tracks, respectively. Proteins used for tracking experiments are CHE-3 tagged with 7x GFP, or DYF-11 tagged with 3x GFP."
NCBI gene IDs:
"kinesin-II: 177685
klp-11: 177685
osm-3: 177141"
Figure legend: "B. Representative two-dimensional IFT tracks from WT and OSM-3(1 or 3xGS) animals. Red and violet circles indicate the start and end of the tracks, respectively."
NCBI gene IDs:
"OSM-3: 177141"
Figure legend: "A. Histogram of anterograde IFT speeds of 3xGFP::CHE-3 at the middle (top) and distal (bottom) ciliary segments (top). Comparisons were performed between the WT and osm-3 mutants. ***"
NCBI gene IDs:
"osm-3: 177141"
Figure legend: "B. Schematic depiction of the C. elegans amphid and phasmid cilia. Each cilium contains a ciliary base, a middle segment (m.s.), and a distal segment (d.s.). C. Amphid (top) and phasmid (bottom) cilia in the WT and osm-3 GS knock-in animals. Cilia are marked with 7xGFP::CHE-3. Arrowheads indicate the ciliary base. Arrows indicate the junctions between the middle and distal segments."
NCBI gene IDs:
"osm-3: 177141"
Figure legend: "D. Cilium length (mean ± SD) in WT and osm-3 mutant animals. Comparisons were performed between the WT and osm-3 mutants."
NCBI gene IDs:
"osm-3: 177141"
Figure legend: "E. Quantifications of dye-filling defects from WT and osm-3 mutant animals."
NCBI gene IDs:
"osm-3: 177141"
Figure legend: "F. Representative TEM images of the middle ciliary segments from osm-3(1xGS) and osm-3(3xGS) worms. Red arrowheads indicate ectopic singlet microtubules. Blue arrowheads indicate ectopic doublet microtubules. The schematics below depict the phenotype of each image."
NCBI gene IDs:
"osm-3: 177141"
Figure legend: "Parkin expression in iBAT from mice exposed to cold (4ºC) for 1 day (1d) or 21 days (21d) compared with control mice (ctl) maintained at 22ºC. Left: Relative transcript levels. Right: representative immunoblot and Parkin protein quantification. Ponceau staining (PS) was used as loading control. (n=6; transcript levels) (n=3; protein levels). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, cold vs. control and ##p&lt;0.01, 1d vs. 21d. One-way ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Relative transcript levels of Park2 in sWAT and eWAT. (n= 6). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, cold vs. control and ###p&lt;0.001, 1d vs. 21d. One-way ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "Relative transcript levels of Parkin in iBAT, sWAT and eWAT of mice injected with the β3-AR agonist CL316,243, every day for 1 week. (n=6). Data are presented as means ±s.e.m. *p&lt;0.05, ***p&lt;0.001, CL316,243 treatment vs. saline. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Relative transcript levels of Park2 and Ucp1 in mature brown adipocytes compared with the stromal vascular fraction (SVF) in iBAT. (n=3). Data are presented as means ±s.e.m. **p&lt;0.01, ***p&lt;0.001. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "mRNA expression profiles of Park2 and Ucp1 during differentiation. (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001 compared to day (d) 4. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "Relative transcript levels of Park2 and Ucp1 in brown adipocytes in culture with fetal bovine serum (FBS, to trigger in vitro differentiation) or charcoal-stripped serum (CSS, non-permissive for differentiation). (n=3). Data are presented as means ±s.e.m. **p&lt;0.01, ***p&lt;0.001. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "Relative transcript levels of Park2 and Ucp1 in brown adipocytes in culture treated with norepinephrine (NE) or cAMP for indicated times. (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001 compared to non-treated cells. One-way ANOVA with Dunnett's post hoc test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "Relative transcript levels of Park2 in brown adipocytes treated with NE in the presence or absence of cycloheximide (CHX). (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "Relative mRNA expression levels of Parkin in brown adipocytes treated with NE in the presence or absence of actinomycin D (n=3). Data are presented as means ±s.e.m. One-way ANOVA with Tukey's post hoc test comparing treatments."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Park2 gene promoter activity in HIB-1B cells transfected with a pGL4-Park2-Luc reporter construct treated with or without NE or cAMP. Data was normalized using Renilla luciferase activity and are expressed as the relative firefly luciferase activity compared to that in untreated cells. (n=6). Data are presented as means ±s.e.m. *p&lt;0.05 compared to non-treated cells. ANOVA with Dunnett's post hoc test"
NCBI gene IDs:
"Luc: 
Park2: 50873"
Figure legend: "Relative transcript levels of Park2 and Ucp1 in brown adipocytes treated with cAMP (12 h) plus H89 (a PKA inhibitor) or SB202190 (a p38 MAPK inhibitor). (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01 with vs. without inhibitor and #p&lt;0.05, and ##p&lt;0.01, ###p&lt;0.001 with vs. without cAMP. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "Relative transcript levels of Park2, and concentration of glycerol in the culture medium of brown adipocytes treated with cAMP (24h) plus Atglistatin (an adipose triglyceride lipase inhibitor) or CAY10499 (a hormone sensitive lipase inhibitor). (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01 ***p&lt;0.001 with vs. without inhibitor and #p&lt;0.05, and ##p&lt;0.01, ###p&lt;0.001 with vs. without cAMP. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "Relative transcript levels of Park2 and Ucp1 in brown adipocytes treated for 24h with a PPARα activator (GW7647), a PPARα inhibitor (GW6471) with or without norepinephrine (NE), or all-trans-retinoic acid (RA). (n=3). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01 ***p&lt;0.001. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Ucp1: 22227"
Figure legend: "Relative transcript levels of Park2 in wild-type and PPARα-KO brown adipocytes treated for 24h with NE (n=3). Data are presented as means ±s.e.m. *p&lt;0.05. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"PPARα: 19013
Park2: 50873"
Figure legend: "Park2 gene promoter activity in HIB-1B cells transfected with a pGL4-Park2-Luc reporter construct and, when indicated, with a pSG5-Ppara expression vector. Cells were treated with the PPARα activator GW7647 or NE. Data was normalized using Renilla luciferase activity and are expressed as the relative firefly luciferase activity compared to that only transfected with the pGL4-Park2-Luc construct (n=6). Data are presented as means ± s.e.m. *p&lt;0.05 compared to non-treated cells. ANOVA with Dunnett's post hoc test."
NCBI gene IDs:
"Luc: 
Ppara: 19013
Park2: 50873"
Figure legend: "Left: Representative optical microscopic images of H&amp;E-stained iBAT (scale bars, 110µm) and electron microscopic images of iBAT (scale bars, 20µm) from wild-type (WT) and Parkin-KO (KO) mice. Right: Quantification of lipid droplet area from electron microscopic images (10 images per condition were used, n =3 WT, n=3 Parkin-KO). Data are presented as means ±s.e.m. *p&lt;0.05. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Relative transcript levels of thermogenesis-related genes of iBAT from WT and Parkin-KO mice exposed to acute cold (4ºC, 24h) compared to those maintained at the control temperature (22ºC). (n=8; WT) (n=6; Parkin-KO). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, CTL vs. cold; #p&lt;0.05, WT vs. Parkin-KO. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Weight gain from WT and Parkin-KO mice fed with chow (CD) or high fat diet (HFD). (n=8; WT) (n=6; Parkin-KO)."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: ", blood glucose levels and plasma insulin levels from WT and Parkin-KO mice fed with chow (CD) or high fat diet (HFD). (n=8; WT) (n=6; Parkin-KO)."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "insulin tolerance test (ITT) from WT and Parkin-KO mice fed with chow (CD) or high fat diet (HFD). (n=8; WT) (n=6; Parkin-KO)."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Relative transcript levels of thermogenesis-related genes in iBAT from WT and Parkin-KO mice given HFD. (n=8; WT) (n=6; Parkin-KO). Data are presented as means ±s.e.m. #p&lt;0.05. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Relative transcript levels of Park2 in iBAT from WT mice fed CD or HFD. (n=8; WT) (n=6; Parkin-KO). Data are presented as means ±s.e.m. Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Park2: 50873
Parkin: 50873"
Figure legend: "WT mice were acclimated to cold for 21 days (21d, 4ºC) and then deacclimated at thermoneutrality (29ºC) for 1 day (1d) or 7 days (7d). Relative transcript levels of thermogenesis-related genes and Park2 in iBAT. (n=6). Data are presented as means ±s.e.m. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001 compared to 21d. ANOVA with Dunnett's post hoc test."
NCBI gene IDs:
"Park2: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Representative immunoblot of LC3B in iBAT, and the loading control (Ponceau staining, PS)."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Immunoblot for p62 and OPTN (left) and its quantification (right) in iBAT. PS was used as the loading control. Arrowhead indicates p62 band and asterisk indicates a non-specific band. (n=4; cold) (n=3; deacclimation). Data are presented as means ±s.e.m. **p&lt;0.01. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Quantification of the total protein amounts of the indicated OXPHOS protein subunits and UCP1 in iBAT. (n=4; cold) (n=3; deacclimation). Data are presented as means ±s.e.m. *p&lt;0.05. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Citrate synthase activity in iBAT protein extracts. (n=6; WT) (n=8; Parkin-KO). Data are presented as means ±s.e.m. *p&lt;0.05. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Mitochondrial DNA (mtDNA) content in iBAT relative to nuclear DNA (nDNA). (n=6; WT) (n=8; Parkin-KO). Data are presented as means ±s.e.m. *p&lt;0.05. ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "Wild-type (WT) and Parkin-KO (KO) mice were acclimated to cold for 21 days (c) and then deacclimated at thermoneutrality (29ºC) for 1 day (d). Representative electron microscopic images of mitochondrial integrity and autophagic/mitophagic events in iBAT after 1 day of cold-deacclimation. Yellow arrowheads indicate mitochondria cristae and blue arrowheads indicate double-membrane autophagosomes. Left panels scale bars, 2 µm. Right panels scale bars, 1 µm."
NCBI gene IDs:
"Parkin: 50873"
Figure legend: "B. Significant increase of survival in Ppt1-/- animals transplanted with HSCPs or BM isolated from Ppt1+/+ mice; Log-rank: HSPCs/BM+/+ tx vs BM-/- tx p&lt;0.001; BM-/- tx vs Ppt1-/- UT p &lt; 0.001."
NCBI gene IDs:
"Ppt1: 19063"
Figure legend: "D. Representative fluorescence microscope photomicrographs showing the distribution of donor-derived cells (transduced with D.NGFR reporter gene, red) and autofluorescent storage material (AF, green) in the brain of a Ppt1-/- mouse euthanized at 240 days of age, after transplantation with HSPCs isolated from Ppt1+/+ mice. Insets in D show high magnification representative confocal scanning photomicrographs of DNGFR+AF+ cells in different regions of the CNS. Arrows show DNGFR+ cells engulfed with autofluorescent (AF) storage material. Scale bar (main figure) = 1mm; scale bar (insets) = 25μm."
NCBI gene IDs:
"D.NGFR: 
Ppt1: 19063"
Figure legend: "G. Representative brightfield photomicrographs of Nissl-stained brain sections from wild type Ppt1+/+ mice and untreated or transplanted Ppt1-/- mice. Black dashed box highlights a portion of the cortex, shown in the high magnification insets. Yellow lines represent the segments applied to measure cortical thickness (see Materials and Methods for details on the quantification). Scale bar (main figure) = 1mm; scale bar (insets) = 25μm. H. Histograms showing the quantification of cortical thickness. SM = somatomotor; SS = somatosensory; V = visual cortex. ** = p &lt; 0.01; Kruskal Wallis followed by Dunn's post-hoc test (for SM) or ANOVA followed by Tukey's post-hoc test (for SS and V). D"
NCBI gene IDs:
"Ppt1: 19063"
Figure legend: "C. Significant increase of the survival in Ppt1-/- mice receiving the administration of hPPT1-LV transduced HSPCs."
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "E, F. Significant prevention of pathological symptoms (DSS higher than 4 and limbs stiffness) in Ppt1-/- mice transplanted with hPPT1-LV transduced HSPCs."
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "E. Representative fluorescence microscope photomicrographs showing autofluorescent storage material (AF, green) in different brain regions of Ppt1-/- mice transplanted with hPPT1-LV transduced HSPCs at 400-420 days of age, when the study was terminated. Mock transplanted and untreated Ppt1 -/- mice at about 250 days, i.e. humane end point, are shown as reference; 450 days-old untreated wild type Ppt1+/+ are shown as control. Neurotrace fluorescent Nissl staining (NT, red) is used to highlight neurons in different brain regions. Arrows highlight the surviving Purkinje cells in the cerebellum of IV and ICV+IV transplanted mice; arrowheads highlight the high number of AF+ cells in the Purkinje cell layer of ICV transplanted or Ppt1-/- untreated (UT) mice. Scale bar = 100μm."
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "A. Quantification of cortical thickness in the somatosensory (SS), somatomotor (SM) and visual (V) area, in untreated Ppt1-/- or Ppt1+/+ mice and in mice transplanted (tx) with hPPT1-LV transduced HSPCs IV, ICV or ICV+IV or mock-transplanted, analyzed at study termination. * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001; **** = p &lt; 0.0001; Kruskal Wallis followed by Dunn's post-hoc test."
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "B. Quantification of CD68 immunoreactivity in the cortex, hippocampus, thalamus and cerebellum of untreated (UT) or gene therapy treated Ppt1-/- mice. * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001; **** = p &lt; 0.00001; Kruskal Wallis followed by Dunn's post-hoc test."
NCBI gene IDs:
"Ppt1: 19063"
Figure legend: "A. Significant increase of the survival in Ppt1-/- mice transplanted at the symptomatic stage with hPPT1-LV transduced HSPCs administered IV+ICV."
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "E. Representative fluorescence microscope photomicrographs showing autofluorescent storage material (AF, green) in different brain regions of Ppt1-/- mice transplanted with hPPT1-LV transduced HSPCs at 18-20 weeks of age, analyzed at study termination. Ppt1 -/- mice mock transplanted at 18-20 weeks of age and analyzed at about 250 days, i.e. humane end point, are shown as reference. Neurotrace fluorescent Nissl staining (NT, red) is used to highlight neurons in different brain regions. Scale bar = 100 μm. F. Top panel: quantification of autofluorescent (AF) storage material in different brain regions of untreated or transplanted mice analyzed at study termination. ** = p&lt;0.01; *** = p&lt; 0.001; **** = p&lt; 0.0001; 2-way ANOVA followed by Tukey's post-hoc test. Bottom panel: histograms showing the quantification of cortical thickness. SM = somatomotor; SS = somatosensory; V = visual cortex. * = p &lt;0.05; ** = p&lt;0.01; **** = p&lt;0.0001; Kruskal Wallis followed by Dunn's post-hoc test (for SM and V area) or ANOVA followed by Tukey's post-hoc test (for SS area). S"
NCBI gene IDs:
"Ppt1: 19063
PPT1: 5538"
Figure legend: "IFM images of KIF13A-YFP transfected control and Rab-knockdown HeLa cells. TN: average tubule number (mean±SEM, n=3). B, C Graphs represent the measurement of KIF13A-positive TN (B) and TL (C) in HeLa cells of Fig 1A (mean± SEM). n=3. nc: total number of cells. nt: total number of tubules.arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs respectively. Scale bars: 10 μm."
NCBI gene IDs:
"Rab: 
KIF13A: 63971"
Figure legend: "IFM images of KIF13A-YFP and mCherry-Rab7A/11A/22A transfected HeLa cells arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs respectively. Scale bars: 10 μm"
NCBI gene IDs:
"mCherry: 
YFP: 
KIF13A: 63971
11A: 8766
22A: 19334
Rab7A: 7879"
Figure legend: "Live cell imaging of GFP/mCherry-Rab22A with respect to mCherry-Rab11A or GFP-Rab7A in HeLa cells. Magnified view of insets (at 0, 20, 40 seconds) are shown separately ), arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs respectively. Scale bars: 10 μm"
NCBI gene IDs:
"GFP: 
mCherry: 
Rab11A: 8766
Rab22A: 19334
Rab7A: 7879"
Figure legend: "IFM images of KIF13A-YFP and mCherry-Rab22AWT/Q64L/S19N cotransfected HeLa cells. Arrowheads and arrows point to the KIF13A-positive tubular REs and E/SEs respectively. The colocalization coefficient (r, in mean±SEM) between two markers was indicated separately. Scale bars: 10 μm Graphs represent the measurement of KIF13A-positive TN (B) and TL (C) in HeLa cells of Fig 2A. n=3. nc: total number of cells. nt: total number of tubules"
NCBI gene IDs:
"mCherry: 
YFP: 
KIF13A: 63971
Rab22A: 19334"