EMBO/SourceData huggingface dataset:
Figure legend: "(C) In vivo limiting dilution assays. miR-10-OE or control cells were subcutaneously injected in the backs of mice at 3 doses (1,000, 10,000 and 50,000 cells for MCF-7 and 5000, 10 000 and 20 000 for SKBR-3). Mice were sacrificed after eight weeks, and tumor burden was analyzed using the ELDA software. Plots are depicted in Fig EV2. The tables show the number of mice with tumors versus the total number of mice injected. Below the tables, the estimated frequency (freq) of stem cells for each group and p is shown. Stem cell frequency for MCF-7 cells was 1 in 34252 (range 94888-12364) in miR-10b-OE cells versus 1 in 238965 (Range 1817801-31414) in miR-scr cells. Stem cell frequency for SKBR3 cells was 1 in 5013 (Range 11135-2257) in miR-10b-OE cells versus 1 in 18778 (Range 45397-7767) in miR-scr cells."
NCBI gene IDs:
"miR-10: 406902///406903
miR-10b: 406903"
Figure legend: "(D) Stemness and EMT marker mRNA levels following miR-10b modulation. miR-10b, OCT4/3, SNAIL and Vimentin expression was quantified in breast cancer cell lines using transient transfection of miR-10b versus a miR-Scr vector (left) or anti-miR-10b versus anti-miR-ctl (right). Log-scale plots show the mean and SEM for the relative expression of each gene from triplicate independent transient transfections, each performed three times. A Mann-Whitney U test was used to compare expression between groups. SOX2, OCT4 and Vimentin were significantly modulated by up- or down-regulation of miR-10b (p=0.0043, p=0.0054 and p=0.000908, respectively). SEM, standard error of the mean."
NCBI gene IDs:
"miR-10b: 406903
OCT4: 5460
OCT4/3: 5460
SNAIL: 6615
SOX2: 6657
Vimentin: 7431"
Figure legend: "(B) MCF-7, SKBR-3 and T47-D breast cancer cells were transfected with gain- or loss-of-function constructs/shRNAs and their respective controls (a miR-10b-OE plasmid or anti-miR-10b). MREs of the validated target HOXD10 and of the putative targets PTEN and PIK3CA were cloned into pMIR-Report, and dual luciferase reporter assays were performed. A mutated control miR-10b (MRE MUT PTEN) was also included. Bars show the mean ± SEM from 6 independent transfections."
NCBI gene IDs:
"HOXD10: 3236
miR-10b: 406903
PIK3CA: 5290
PTEN: 5728"
Figure legend: "(C) PTEN and PIK3CA expression after miR-10b modulation. Left panel shows miR-10b, PTEN and PIK3CA expression by qRT-PCR in MCF-7 cells with ectopic miR-10b expression. Right panel shows miR-10b, PTEN and PIK3CA expression by qRT-PCR in MDA-MB-231 cells after antagomiR-10b transfection. Data are from three independent transient transfections performed by triplicate."
NCBI gene IDs:
"miR-10b: 406903
PIK3CA: 5290
PTEN: 5728"
Figure legend: "(D) To further confirm PTEN targeting, we performed gain- or loss-of-function experiments in breast cancer cell lines (MCF-7, MCF-7/CD44+, SKBR-3 and T47D) by overexpressing miR-10b or inhibiting it with a miR-10b-OE plasmid or anti-miR-10b shRNAs. PTEN and miR-10b levels were measured by qRT-PCR. The log2-fold change of PTEN (left panel) and PIK3CA (right panel) mRNA levels obtained through the 2-ΔΔCT method were plotted against their respective miR-10b expression levels. Correlation analysis was performed [PTEN (r=-0.80), PI3KCA (r=0.43)]. A two-tailed Student's t test was used to compare the two groups (*p &lt; 0.05). SEM, standard error of the mean."
NCBI gene IDs:
"miR-10b: 406903
PIK3CA: 5290
PTEN: 5728"
Figure legend: "(E) PTEN protein modulation by miR-10b. Upper panel: PTEN protein levels in SKBR-3, and MCF-7 cells were analyzed by Western blot. GAPDH was used as a loading control with cells cultured as in (B). Lower panel: Densitometric analysis of Western blot assays performed with three biological replicates."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "(F) PTEN immunohistochemistry analysis of MCF-7-miR-10b-OE or control MCF-7-miR-Scr-OE tumorxenografts."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "(G) miR-10b overexpression does not increase mammosphere formation in PTEN-depleted cells. MCF-7-miR-Scr-OE cells (left panels) and MCF-7-miR-10b-OE (right panels) were transfected with a Dicer-substrate RNA directed toward PTEN, and after 24 h, quantitative RT-PCR assays for PTEN, SOX2, OCT4, SNAIL and Vimentin were performed. Additionally, the same cells were analyzed for mammosphere formation. The graph shows the mean and standard error of the relative increase of each marker or number of mammospheres in PTEN-depleted cells (black bars) and in cells transfected with a scrambled control (white bars) in three independent biological experiments performed by triplicate."
NCBI gene IDs:
"Dicer: 23405
miR-10b: 406903
OCT4: 5460
PTEN: 5728
SNAIL: 6615
SOX2: 6657
Vimentin: 7431"
Figure legend: "(H) T47-D cells were transfected with antagomiR-10b, a dsi-RNA for PTEN or both and mammospheres produced as described in material and methods. Bars show mean and SEM from three independent experiments. * p=0.003 by Student´s T test, after ANOVA showing statistical differences in groups."
NCBI gene IDs:
"dsi: 23405
miR-10b: 406903
PTEN: 5728"
Figure legend: "(I) PTEN overexpression compensates for miR-10b overexpression. Left panel: MDA-468 cells were transfected with a plasmid overexpressing wild-type PTEN or a version containing a mutated MRE in its 3'-UTR, with or without a plasmid containing miR10b and PTEN mRNA expression was measured by end-point RT-PCR. Middle panel: Relative expression of stemness factor SNAIL. The plots show the relative expression from biological triplicate analyses (mean and SE shown). Right panel: Relative expression of stemness factor SOX2. The plots show the relative expression from biological triplicate analyses (mean and SE shown)."
NCBI gene IDs:
"miR-10b: 406903
miR10b: 406903
PTEN: 5728
SNAIL: 6615
SOX2: 6657"
Figure legend: "(B) Upper panel. Cultures were serum-starved for 18 h and then stimulated with insulin or exposed to the PI3K pathway inhibitors Wortmannin or LY294002. PTEN, PI3KCA, GSK3 and phospho-AKT Ser-473 protein levels were analyzed by Western blot. GAPDH was used as a loading control. AKT kinase activity was analyzed in these cells by immunoprecipitating phosphorylated AKT proteins with a p-AKT-Ser-473 antibody and using chimeric GSK-3 as a substrate. A two-tailed Student's t test was used to compare the two groups (p&lt;0.05). SEM, standard error of the mean. Lower panel. SKBR3 cells were transiently transfected with a plasmid containing an open reading frame of PTEN, miR-10b or both and phospho-AKT, AKT or tubulin levels analyzed by Western Blot assays. Control is empty vector-transfected cells."
NCBI gene IDs:
"miR-10b: 406903
PTEN: 5728"
Figure legend: "(C) Clonogenic ability of miR-10b-depleted CD44+ MCF-7 cells after PI3K pathway inhibition. Cultures were serum-starved for 18 h and then stimulated with insulin or exposed to the PI3K pathway inhibitors wortmannin or LY294002. Cells transfected with anti-miR-10b or a scrambled control (anti-miR-ctl) were seeded in soft agar at clonal density and exposed to insulin or PIK3CA inhibitors. After 3 weeks, colonies were quantified. Bars show the mean ± SEM for 3 independent experiments."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "(D) miR-10b overexpression does not induce additional changes in cell colony numbers in response to insulin or PI3K inactivation. MCF-7 stable miR-Scr-OE or miR-10b-OE cells were seeded in soft agar at clonal density and exposed to insulin or PIK3CA inhibitors. After 3 weeks, colonies were quantified. Bars show the mean ± SEM for 3 independent experiments."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "HIF-1α ChIP-seq signal in the HIF-2α KO cells was plotted against that in the wild-type cells for all canonical HIF-1 binding sites in either cell type"
NCBI gene IDs:
"HIF-2α: 2034"
Figure legend: "HIF-2α ChIP-seq signal in the HIF-1α KO cells was plotted against that in the wild-type cells for all canonical HIF-2 binding sites in either cell type. No significant increase (Wilcoxon rank sum test) in binding of either isoform was observed following deletion of the other"
NCBI gene IDs:
"HIF-1α: 3091"
Figure legend: "Box-and-whisker plots showing: (D) The effect of HIF-2α inactivation on HIF-1α ChIP-seq signal at sites that specifically bound HIF-2α, but not HIF-1α in wild-type cells showing a slight, but significant (p = 4 x 10-5, Wilcoxon signed rank test) decrease rather than increase (compare red boxes) consistent with the reduction in HIF-1α protein observed in panel A"
NCBI gene IDs:
"HIF-2α: 2034"
Figure legend: "The effect of HIF-1α inactivation on HIF-2α ChIP-seq signal (compare blue boxes) at sites that specifically bound HIF-1α, but not HIF-2α in wild-type cells showing no significant effect (p = 0.5, Wilcoxon signed rank test)"
NCBI gene IDs:
"HIF-1α: 3091"
Figure legend: "The effect, at sites that bind both isoforms in wild-type cells, of (F) HIF-2α inactivation on HIF-1α binding intensity showing a similar small but significant reduction rather than increase (p = 2 x 10-16, Wilcoxon Signed rank test) as abov"
NCBI gene IDs:
"HIF-2α: 2034"
Figure legend: "The effect, at sites that bind both isoforms in wild-type cells, o HIF-1α inactivation on HIF-2α binding intensity showing no significant effect (p = 0.4, Wilcoxon signed rank test)"
NCBI gene IDs:
"HIF-1α: 3091"
Figure legend: "Parental and TGF-β isogenic cell lines were treated with 50nM volasertib for 72 hours. Cells were then harvested, and lysates were immunoblotted for cleaved PARP and γH2AX proteins were subsequently quantitated and normalized with β-actin (B)."
NCBI gene IDs:
"TGF-β: 7040"
Figure legend: "Calu6, H157, and H1355 TPR-Met-expressing or vector control (pBABE)-expressing cells were treated as indicated for 24 hours and apoptosis was measured using the Apo-BrdU assay."
NCBI gene IDs:
"Met: 4233
TPR: 7175"
Figure legend: "Parental and TPR-Met-expressing cell lines were treated with the indicated drugs for 24 hours. Cells were then harvested, and lysates were immunoblotted for the indicated proteins. β-actin was used as a loading control."
NCBI gene IDs:
"Met: 4233
TPR: 7175"
Figure legend: "Parental and TPR-Met-expressing cell lines were treated as indicated for 24 hours and allowed to grow in drug-free medium for 15-20 days to form colonies, which were counted using ImageJ. Data are means ± standard error of the mean from three independent experiments. Significant differences using two-way analysis of variance with Bonferroni or Benjamini-Hochberg correction for multiple comparisons are indicated."
NCBI gene IDs:
"Met: 4233
TPR: 7175"
Figure legend: "Mesenchymal NSCLC cell lines with the noted MET alterations were incubated with 50nM volasertib for four hours and subjected to immunoblotting with the indicated antibodies. *p &lt; 0.05. **p &lt; 0.01."
NCBI gene IDs:
"MET: 4233"
Figure legend: "Basal protein and mRNA expression in parental and VAR cells for the indicated proteins was measured using immunoblotting (upper) and reverse-transcriptase PCR (lower), with GAPDH expression used for normalization."
NCBI gene IDs:
"GAPDH: "
Figure legend: "Epithelial and mesenchymal NSCLC cell lines after Plk1 silencing with siRNA for 48 hours. Cells were then harvested, and lysates were immunoblotted for the indicated proteins."
NCBI gene IDs:
"Plk1: 5347"
Figure legend: "Calu6 and H1792 mesenchymal NSCLC cell lines after VIM silencing with siRNA for 48 hours. Cells were then harvested, and lysates were immunoblotted for the indicated proteins."
NCBI gene IDs:
"VIM: 7431"
Figure legend: "Calu6 (mesenchymal) and H1975 (epithelial) NSCLC cell lines after Plk1 and INTB1 silencing with siRNA for 48 hours. Cells were then harvested, and lysates were immunoblotted for the indicated proteins."
NCBI gene IDs:
"INTB1: 3688
Plk1: 5347"
Figure legend: "A, B, and C. Immunostaining of Tom20 (mitochondria) in cells positive for β3-tubulin in DIV6 Ifnb+/+ and Ifnb-/- CNs with or without rIFN-β for 24 h. A. Projection of 3D image of Tom20. Scale bars equal 20 microns. B. Quantification of total mitochondrial mass per neuron. C. Mean individual mitochondria volume in Ifnb+/+ and Ifnb-/- CNs. "
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "D, E, F, and G. 3D electron microscopy of thalamus in 12-month-old Ifnb+/+ and Ifnb-/- mice. D. Volume rendering of mitochondria (left panel) and corresponding mitochondria in 2D electron microscopy image (right panel) in cell bodies. Each individual mitochondrion is colored differently. Scale bars equal 2 microns. Voxel size equals 0.00878908x0.00878908x0.03 microns for the Ifnb+/+ neuron and 0.00439453x0.00438453x0.04 microns for Ifnb-/-. Nuclei are in grey and marked with 'N.' E. Reconstituted axonal mitochondria. Images extracted from Movies EV1 and 2. F. Size of individual mitochondria in cell bodies and non-myelinated and myelinated axons. N&gt;30; error bars are SD. G. Number of branches per mitochondrion. N&gt;30; error bars are SD. "
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "D-E. Respiration of isolated mitochondria from Ifnb+/+ and Ifnb-/- whole brains. OCR measurements under Complex 1 substrates (pyruvate/malate) were obtained at baseline (B) and on addition of ADP, oligomycin, FCCP and rotenone/antimycin A to capture S3, S4o, S3u, and nonmitochondrial respiration. N=3-4 mice per group; bar graphs show mean + SE."
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "K. Immunofluorescence of 8OHdG in CN cultures. Neurons were labeled with Nissl. Scale bars equal 50 microns. L. Quantification of 8OHdG by ELISA in brain tissues in young (aged 1.5-3 months) and old (aged 6-12 months) Ifnb+/+ and Ifnb-/- mice. "
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "M. Immunoblot for optineurin in 6-week-old midbrains of Ifnb+/+ and Ifnb-/- mice. N. Immunoblot for optineurin in DIV6 Ifnb+/+ and Ifnb-/- CNs with or without 30 U/mL rIFN-β."
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "I. MFN2 and OPA1 immunoblots and quantification in Ifnb+/+ and Ifnb-/- CN cultures and quantification. Vinculin was used as a loading control. Error bars are SEM from 3 independent experiments."
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "J. Optineurin and Tom20 immunofluorescence in Ifnb+/+ and Ifnb-/- MEFs transfected with Drp1 or phosphomimetic Drp1."
NCBI gene IDs:
"Drp1: 74006
Ifnb: 15977"
Figure legend: "E. Volume rendering from 3D TEM images of Ifnb+/+ and Ifnb-/- thalami. Mitochondria are blue, the ER is yellow, and nuclei are grey. Mitochondria undergoing fission are cyan and indicated with a white arrow. 2D pictures are shown in Fig 2."
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "H. Projections of 3D images from immunostaining of Drp1 (blue), Hsp60 (mitochondria, green), INF2 (yellow), and calnexin (ER, magenta) in Ifnb-/- MEFs with or without rIFN-β treatment for 6 h. Scale bar equal 2 microns. Arrows indicate fission foci. I. Quantifications of triple-positive MT-DR+Drp1+Cnx+ in MEFs. Images are shown in Fig EV3. "
NCBI gene IDs:
"Ifnb: 15977"
Figure legend: "D. Projections of 3D images from immunostaining for phospho-S622 Drp1 (yellow), Hsp60 (mitochondria, green), and calnexin (ER, magenta) in N2A cells depleted of STAT5 A and B (δStat5) or IFN-β (δIfnb) or with nontargeting control (NTC). Cells with or without rIFN-β treatment for 6 h. Scale bar equals 5 microns. Arrows indicate triple-positive foci. E. Quantification of D. "
NCBI gene IDs:
"δIfnb: 15977
STAT5 A: 20850"
Figure legend: "M. Optineurin immunostaining in Ifnb+/+ and Ifnb-/- MEFs overexpressing PGAM5 and quantification. Empty vector was used as control. Additional controls are shown in Fig EV5L. Error bars are SD from N=30 cells."
NCBI gene IDs:
"Ifnb: 15977
PGAM5: 72542"
Figure legend: "B) Immunoblot of NDC80 levels following depletion of the Ndc80 complex by RNAi."
NCBI gene IDs:
"Ndc80: 10403"
Figure legend: "B. Representative immunoblot of transfected missense ROS1 TKD mutations in transfected HEK-293A lysates."
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "C. Densitometry analysis of immunoblots (N = 3, biological replicates) as represented in (B). Fold-change in relative ROS1 phosphorylation is calculated by first calculating the ratio of phospho-ROS1 to total ROS1 protein for each variant and then normalizing to ROS1WT. One-way ANOVA and Sidak's test were used to control for multiple comparisons in C Error bars in figure represent mean ± SEM."
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "D. Representative immunoblot of ROS1 D2113 position substitutions (E, G, Y, and Q as indicated) in HEK-293A cells. E. Densitometry of immunoblots (N = 3, biological replicates) as represented in (D). pROS1- phospho-ROS1, tROS1- total-ROS1. One-way ANOVA and Sidak's test were used to control for multiple comparisons in E. Error bars in figure represent mean ± SEM."
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "A. Immunoblot analysis of phospho-ROS1 and total ROS1 protein expression of NIH-3T3 cells stably transduced with empty vector (EV) or indicated ROS1 TKD mutants"
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "C. Soft-agar assay of NIH-3T3 ROS1D2113N compared with NIH-3T3 SLC-ROS1 fusion cells (positive control) or ROS1K1980E (kinase-dead, negative control). +/- lorlatinib treated samples were compared for significance using two-way RM ANOVA (matching across rows, N = 3, biological replicates) with Šídák's multiple comparisons test (P values are indicated in figure)"
NCBI gene IDs:
"SLC: 
ROS1: 6098"
Figure legend: "D. Immunoblot analysis of phospho-ROS1 and total ROS1 protein expression in MCF10A cells stably transduced with indicated ROS1 variants"
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "Cell proliferation data showing effects of treatment with DMSO (Vehicle), crizotinib (250nM), lorlatinib (250 nM), and NVL-520 (250 nM) in MCF10A ROS1 wildtype (WT) (G), D2113N (H), D2113G (I) and SLC34A2-ROS1 (SLC-ROS1) (J) as indicated. Ordinary One-way ANOVA with Dunnett's multiple comparisons test was used to determine significant differences. N = 6 wells, biological replicates (H-I). Error bars in figure represent mean ± SEM."
NCBI gene IDs:
"SLC: 
SLC34A2: 
ROS1: 6098"
Figure legend: "A-B. Volcano plots of statistical discoveries from IMAC-enriched (A) and phospho-tyrosine-enriched (B) phosphoproteomic spectral counts comparing ROS1D2113N data to ROS1WT. Select phosphosites linked to indicated cellular functions are annotated within the graph. Unpaired t tests of log2 transformed counts from ROS1WT and ROS1D2113N cells were conducted using Graphpad Prism with variance assumption of individual variance for each role (N=3, biological replicates). Multiple Comparison tests used False Discovery Rate (FDR) with two-stage step-up (Benjamini, Krieger, and Yekutieli) with desired FDR of 5%. X axis features log2-transformed ratio of normalized intensities of ROS1D2113N relative to ROS1WT while y axis features corresponding log10(q) values."
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "A. Immunoblotting was performed on cell lysates generated via transient transfection of the following plasmids: pCX4 ROS1 wildtype (WT), ROS1D2113G (D2113G), ROS1D2113N (D2113N) and the ROS1 fusions, CD74-ROS1, EZR-ROS1 and SLC34A2-ROS1 (SLC-ROS1). The targets interrogated are indicated in the labels on the left side of the image panels and the catalog numbers as well as dilution for antibodies are reported in Methods. B. Immunoblot densitometry graphs to quantitatively compare activation of ROS1, SHP2, ERK1/2 and STAT3 (N = 2, biological replicates). Fusions are listed only by fusion partner: CD74-ROS1 (CD74), EZR-ROS1 (EZR), SLC34A2-ROS1 (SLC)."
NCBI gene IDs:
"EZR: 
SLC: 
SLC34A2: 
CD74: 972
ROS1: 6098"
Figure legend: "C. Immunoblotting was performed on cell lysates generated from stable HEK293A ROS1D2113N, HEK293A CD74-ROS1 and NIH ROS1D2113N and NIH3T3 CD74-ROS1 after treatment with ROS1 TKI. HEK293A stable cell lines were treated with DMSO, 100 nM entrectinib and 100 nM lorlatinib, and NIH3T3 stable cell lines were treated with 25 nM and 100 nM lorlatinib. The targets interrogated are indicated in the labels on the left side of the image panels in HEK293 images and on the right side of image panels in NIH3T3 images. D. Immunoblot densitometry graphs to quantitatively compare extent of inhibition ROS1, SHP2, ERK1/2 and STAT3 and AKT1 phosphorylation from two independent replicate experiments. Pixel density of phosphorylated protein signal was divded by total protein signal, and this ratio was internally normalized to DMSO (Vehicle) treated cell ratio. Note that only the 100 nM lorlatinib condition that was common to the HEK293A and NIH3T3 experiments is depicted in graphs."
NCBI gene IDs:
"CD74: 972
ROS1: 6098"
Figure legend: "B. Kaplan-Meier survival curve of mice described in (A). Comparison of survival curves using Log-rank (Mantel-Cox) test was used to compare difference between EV (N = 5) and D2113N (N = 5), and EV and SLC34A2-ROS1 (N = 4); significance is indicated as P values within graph."
NCBI gene IDs:
"SLC34A2: 
ROS1: 6098"
Figure legend: "G. Immunoblot analysis of the phosphorylated (p) and total (t) proteins from NIH-3T3 ROS1D2113N P1 cell lysates prepared from cells treated with vehicle (DMSO) or 10, 100, or 500 nM of crizotinib, entrectinib, or lorlatinib for 4 hours."
NCBI gene IDs:
"ROS1: 6098"
Figure legend: "LMS (red) and HMS (black) gene expression quantification in Epi cells by random-primed RT-qPCR relative to RPL11 and represented as log2 fold change to CTR. Each dot corresponds to an independent replicate, bars represent Mean ± Standard Deviation values."
NCBI gene IDs:
"RPL11: 6135"
Figure legend: "Metagene profiling within the 10 kb window around TSS of H3K27ac, LSD1 and H3K4me2 over IgG in Epi cells expressing none (CTR) or different HOTAIR variants (HOT, HOTΔP and HOTΔL). bp stands for base pairs."
NCBI gene IDs:
"HOT: 100124700
HOTAIR: 100124700"
Figure legend: "Metagene profiling of H3K27ac, LSD1 and H3K4me2 over IgG within the 5 kb window of enhancers in Epi cells expressing none (CTR) or different HOTAIR variants (HOT, HOTΔP and HOTΔL). bp stands for base pairs."
NCBI gene IDs:
"HOT: 100124700
HOTAIR: 100124700"
Figure legend: "B) Western blot detection of DOT1L  and TUBULIN  (TUB) in whole testicular  protein extracts from CTL and Dot1l-KO (KO) adult mice."
NCBI gene IDs:
"Dot1l: 208266"
Figure legend: "D) Western blot  detection of DOT1L  and H3K79me2 in Dot1l-KO (KO) and CTL germ cells. Normalization was performed by detecting the membrane with anti-TUBULIN  (TUB). SC  = primary spermatocytes, RS = round spermatids."
NCBI gene IDs:
"Dot1l: 208266"
Figure legend: "B) Histology  of Dot1l-KO and CTL testes  (periodic acid-Schiff staining). Scale bars indicate 200µm."
NCBI gene IDs:
"Dot1l: 208266"
Figure legend: "D) Results from the  tests  of fertility (natural mating) of Dot1l-KO and CTL males  (7 males  in each group mated for ~3 months with wild-type females). Schematic diagrams representing the number of litters  and the litter  size from progeny of Dot1l-KO or CTL males  (mean ± SEM).The p-value obtained with a Student t-test is indicated on each graph."
NCBI gene IDs:
"Dot1l: 208266"
Figure legend: "C) Scatter plots (mean values following angular transformation ± SEM) of the percentage of motile and progressively motile spermatozoa  in Dot1l-KO and CTL adult mice  (N= 12 biological replicates for CTL and 10 biological replicates for Dot1l-KO) obtained following CASA  (computer-assisted sperm analysis). ***p-value &lt;0.0005 (Mann Whitney test performed after angular transformation of the percentages)."
NCBI gene IDs:
"Dot1l: 208266"
Figure legend: "F) Western blot  detection of  transition protein 2  (TNP2) in spermatozoa  from Dot1l-KO and CTL males. The same quantity of material was loaded in each well (i.e. extracts from 2 million spermatozoa)."
NCBI gene IDs:
"Dot1l: 208266"