EMBO/SourceData huggingface dataset:
Figure legend: "WT, and Riplet   KO HEK293  cells were stimulated with 200 ng/ml of short poly I:C. The expression of SP100, PML, and ANKRD1  mRNA was determined by RT-qPCR  and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt;0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"ANKRD1: 27063
GAPDH: 2597
PML: 5371
Riplet: 84282
SP100: 6672"
Figure legend: "B) WT, LGP2  KO, HEK293  cells were stimulated with 200 ng/ml of short poly I:C. The expression of SP100, PML, and ANKRD1  mRNA was determined by  RT-qPCR  and normalized to GAPDH  (n = 3, two-way ANOVA, *p &lt;0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"ANKRD1: 27063
LGP2: 79132
GAPDH: 2597
PML: 5371
SP100: 6672"
Figure legend: "C, WT and Riplet  KO A549  (C) cells were stimulated with 200 ng/ml of short poly I:C. The expression of SP100  was determined by RT-qPCR (n = 3, two-way ANOVA, *p &lt; 005). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"Riplet: 84282
SP100: 6672"
Figure legend: "D) WT and Riplet  KO MEF  (D) cells were stimulated with 200 ng/ml of short poly I:C. The expression of SP100  was determined by RT-qPCR (n = 3, two-way ANOVA, *p &lt; 005). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"Riplet: 71956
SP100: 20684"
Figure legend: "E) WT and Riplet  KO BMM  were infected with influenza A virus (Flu) at MOI = 10. PML  and SP100  mRNA expression was determined by  RT-qPCR (n = 3, two-way ANOVA, *p &lt; 0.05). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"PML: 5371
Riplet: 84282
SP100: 6672"
Figure legend: "F) WT and Riplet  KO A549  cells were infected with  influenza A virus infection at MOI = 10. WCE was prepared at the indicated time points  and subjected to SDS-PAGE. Proteins were detected by  western blotting  with the indicated antibody."
NCBI gene IDs:
"Riplet: 84282"
Figure legend: "G, LGP2  or LGP2-4KR stably expressing WT or Riplet  KO HEK293  cells were infected with SeV  (G) IFN-β mRNA levels at indicated  time points were determined by  RT-qPCR. (n = 3, two-way ANOVA, *p &lt; 0.05, ns: not significant (p &gt; 0.05)). Right graph in panel G is an enlargement of Y-axis of the left graph (dotted line). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
IFN-β: 3456
Riplet: 84282"
Figure legend: "H) LGP2  or LGP2-4KR stably expressing WT or Riplet  KO HEK293  cells were stimulated with 200 ng/ml of short poly I:C (H).  IFN-β mRNA levels at indicated time points were determined by  RT-qPCR. (n = 3, two-way ANOVA, *p &lt; 0.05, ns: not significant (p &gt; 0.05)). Data information: All data are representative at least two independent experiments, and \"n\" represents the number of samples (biological replicates). Error bars represent standard deviation from the mean."
NCBI gene IDs:
"LGP2: 79132
IFN-β: 3456
Riplet: 84282"
Figure legend: "(D) Western blot of whole zebrafish larvae, Smyhc1 stained with the F59 antibody. smyhc1+/+ larvae express Smyhc1 at 24hpf, but not at 48hpf. smyhc1-/- larvae do not express Smyhc1."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(E) Smyhc1 immunohistochemistry stain of 24 hpf (hour post fertilization) zebrafish larvae. Filamentous actin is stained with phalloidin (red). Nuclei are stained with DAPI (blue). Smyhc1 is stained with the F59 antibody (green) (Elworthy et al, 2008). smyhc1+/+, smyhc1R673H/+, and smyhc1R673H/R673H larvae display Smyhc1 in the muscle fibers at 24hpf. smyhc1-/- larvae at 24 do not stain positively for Smyhc1. Scale bar represents 50 µm."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(A) Morphologies of smyhc1+/+, smyhc1-/+ and smyhc1-/- embryos are grossly normal at 2 days post fertilization (dpf), despite complete paralysis of smyhc1-/- embryos. In contrast, the smyhc1R673H/+ embryos have notochord kinks in larval stages (arrow head), while smyhc1R673H/R673H embryos have much more severe notochord kinks that compress and distort the body axis (arrow heads). The smyhc1R673H/- embryos completely phenocopy smyhc1R673H/R673H embryos. (B) Quantification of smyhc1R673H embryonic phenotypes at 2 dpf. "
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(B) The predicted mendelian (1:2:1) and experimental ratio of genotypes of progeny from smyhc1R673H/+ in-crosses demonstrates survival at the indicated ages. This ratio quickly skewed toward an overrepresentation of smyhc1+/+ fish with no representation of smyhc1R673H/R673H after 5 dpf. There is also dropout of smyhc1+/R673H fish into adulthood. Number of fish counted per group is indicated."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(A) Gross morphology of smyhc1 mutant adults. Most smyhc1-/- adult fish display dorsal tail curvature, while smyhc1R673H/+ adults have a shortened body axis and variable spinal curves."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(B) Alizarin red staining of bone shows spinal curvature but no bony fusions in the skeleton of smyhc1-/- adults, in contrast to the compression and fusion of vertebrae seen in smyhc1R673H/+ adults. Distal tail regions are highlighted and enlarged."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(A) Time course of light-triggered larval movements were manually counted at indicated times between 24 and 48 hpf. The smyhc1-/- larvae (n = 36) were paralyzed up to 48 hpf. smyhc1R673H/+ mutants (n = 15) twitched slightly less than their smyhc1+/+ siblings (n = 21) at 34hpf. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(B) Distance travelled during 5 minutes of spontaneous swimming at 6 dpf quantified by motion tracking software (Noldus Ethovision). At 6 dpf, both smyhc1-/- (n = 24) and smyhc1R673H/+ (n = 11) larvae travelled significantly less than smyhc1+/+ larvae (n = 13). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(D) Time spent swimming in swim tunnel before fatigue in adults at 6 months post fertilization (mpf). smyhc1+/+ (n = 6), smyhc1-/- (n = 5), smyhc1R673H/+ (n = 5)."
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(E) Myoseptal intervals of slow skeletal muscle at 1 dpf in smyhc1+/+ (n = 54), smyhc1-/- (n = 31), smyhc1R673H/+ (n = 45), and smyhc1R673H/R673H (n = 49). Distance between myosepta was measured perpendicular to rostral-caudal body axis at defined mid-body regions in phalloidin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range. (F) Myoseptal intervals of slow skeletal muscle at 3 dpf in smyhc1+/+ (n = 51), smyhc1-/- (n = 32), smyhc1R673H/+ (n = 64), and smyhc1R673H/R673H (n = 38). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range. "
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(G) Z-disc intervals (sarcomere length) of slow skeletal muscle at 1 dpf in smyhc1+/+ (n = 380), smyhc1R673H/+ (n = 293), and smyhc1R673H/R673H (n = 268). Distance between z-discs was measured in anti-α-actinin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range. (H) Z-disc intervals (sarcomere length) of slow skeletal muscle at 3 dpf in smyhc1+/+ (n = 249), smyhc1-/- (n = 278), smyhc1R673H/+ (n = 246), and smyhc1R673H/R673H (n = 252). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range. "
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "(A) Representative morphologies of smyhc1+/+, smyhc1R673H/+, and smyhc1R673H/R673H larvae at 2 dpf after 24 hour treatment with 0.25% DMSO (control) or 25 μM para-aminoblebbistatin. All larvae treated with para-aminoblebbistatin develop a slight dorsal tail curve and pericardial edema regardless of genotype. All para-aminoblebbistatin-treated smyhc1R673H/+ (n=9) and smyhc1R673H/R673H (n=3) larvae are indistinguishable from smyhc1+/+ unlike the untreated larvae which have markedly curved or distorted body axis. (B) Representative images of smyhc1+/+, smyhc1R673H/+, and smyhc1R673H/R673H larvae at 3dpf after 48 hour treatment with 0.25% DMSO (control) or 25 μM para-aminoblebbistatin. All larvae treated with para-aminoblebbistatin develop a slight dorsal tail curve and pericardial edema regardless of genotype. Para-aminoblebbistatin-treated smyhc1R673H/+ (n=9) and smyhc1R673H/R673H (n=3) larvae are indistinguishable from smyhc1+/+ unlike the untreated larvae which have markedly curved or distorted body axis. "
NCBI gene IDs:
"smyhc1: 321552"
Figure legend: "A. In response to a 1 s light pulse, cac alleles lack on transients at day 3 and day 33."
NCBI gene IDs:
"cac: 32158"
Figure legend: "H. The level of p62 is increased in cacJ brain. I. Quantification of H. Data are presented as means ± SEM. (*: p &lt; 0.05)."
NCBI gene IDs:
"cac: 32158"
Figure legend: "Autophagy defects observed in cac mutants are not due to defects in neurotransmitter release, but due to defects in lysosomal fusion.TEM sections of 1-day-old and 30-days-old photoreceptor terminals of flies raised in 12 h light/dark conditions. A, B, D, and E. stj and Vha100-1 (V0) mutant terminals accumulate AVs upon aging. G, H. n-Syb mutant photoreceptor terminals show increased SVs, but not a significant accumulation of AVs and fusion-primed AVs. J, K, M and N. Vamp7 and fab1 mutant photoreceptor terminals show the highest increase in AV accumulation. C, F, I, L, O. Quantification of the different AVs for each genotype (**: p &lt; 0.01). Eyes from three animals for each genotype at each age were analyzed and the counted cartridge number were listed as \"n = \" in the figure. Data are presented as means ± standard deviation (SD). Scale bars, 1 μm."
NCBI gene IDs:
"cac: 32158
fab1: 37033
n-Syb: 38196
stj: 36526
Vamp7: 36015
Vha100–1: 43442"
Figure legend: "A. Calbindin and hematoxylin staining of mice cerebella indicates that Cacna1atg-la mice have progressive PC and granule cell loss upon aging. Scale bar, 200 μm."
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: ". B, D. Calbindin (green) and 4',6-diamidino-2-phenylindole (DAPI)(blue) staining of micecerebellum indicates that there are swollen PC axons (arrows) in the granular layers of the Cacna1atg-lamicecerebellum at day 25 (B) and the Cacna2d2du-2Jmice at day 50 (D). Scale bar, 20 μm"
NCBI gene IDs:
"Cacna1a: 12286
Cacna2d2: 56808"
Figure legend: "C, E. The TEM of the axons at the granular layers of the cerebellum showing that many PC axons in Cacna1atg-la and Cacna2d2du-2J mice are swollen and accumulated with numerous mitochondria, autophagosome/MVBs-like structures (red arrows), and various cytoplasmic vesicles or aberrant membrane structures, including stacks of cisternal membranes (blue arrows), and folded ER (yellow arrows). Scale bars for right two panels are 2 μm. Other scale bars are 500 nm"
NCBI gene IDs:
"Cacna1a: 12286
Cacna2d2: 56808"
Figure legend: "F, G. LC3II and p62 are slightly increased in 15-days-old Cacna1atg-la mice and 50-days-old Cacna2d2du-2J mice. b, d are quantification of a and c. Data are presented as means ± SEM. (*: p &lt; 0.05)."
NCBI gene IDs:
"Cacna1a: 12286
Cacna2d2: 56808"
Figure legend: "A. CACNA1A co-localizes with LAMP1 in primary cerebellar cultures of both CTL and Cacna1atg-la mutants. Scale bars, 20 μm."
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: "B. CACNA1A is present as punctae on the Vacuolin-1 enlarged LAMP1 positive lysosomes in primary cerebellar cultures of both CTL and Cacna1atg-la mutants. Scale bars, 20 μm."
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: "A. LysoTracker Red staining of the primary cerebella neurons from Cacna1atg-la mice and WT controls. The control cells show big bright punctae, while the mutant cells have much dimmer and smaller punctae. B, C and D. Quantification of LysoTracker relative intensity (\"n\" represents the counted cell number), LysoTracker positive punctae number and size per cell (\"n\" represents the counted image number)"
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: ". E. Lysosomal marker LAMP1 co-localizes with autophagosome marker LC3 in CTL but not Cacna1atg-la mutant cells. Scale bar, 20 μm. F. Quantification of LC3 and LAMP1 co-localization. Data are presented as means ± SD. (ns: not significant; ***: p &lt; 0.001)."
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: "A. DQ-BSA labeled late endosomes fuse with LAMP1 labeled lysosomes in CTL but not the mutant cells. Scale bar, 20 μm. B. Quantification of DQ-BSA and LAMP1 co-localization in CTL and Cacna1atg-la cells. Data are presented as means ± SD. (ns: not significant; ***: p &lt; 0.001) C. Bepridil but not ω-agatoxin TK (ω-Aga TK) treatment reduces lysosomal fusion in cultured cerebellar cells. DQ-BSA (Green) labeled late endosomes co-localize with LAMP1 (Red) labeled lysosomes with/without ω-Aga TK (1 µM) or Bepridil (10 µM) treatment. Scale bar, 20 μm. D. Quantification of DQ-BSA and LAMP1 co-localization with ω-Aga TK or Bepridil treatment. Data are presented as means ± SD. (ns: not significant; **: p &lt; 0.001)."
NCBI gene IDs:
"Cacna1a: 12286"
Figure legend: "A, B Temporal expression of Snail genes in the intestinal tract in (A) embryonic E12.5 to E18.5 and (B) postnatal P0 to P20 measured by qRT-PCR. Shown are means ± SD."
NCBI gene IDs:
"Snail: 20613"
Figure legend: "C, E, G, I Immunohistochemical staining for Snai1/2 (Abcam ab85931) (C) E14.5midgut, (E) E14.5hind gut, (G) postnatal day 14 SI, (I) postnatal day 14 colon. Snai1immunohistochemistry peptide competition controls are shown as inserts (C', E', G', I'). Scale bars: 20 mm.D, F, H, J, K X‐gal staining for β‐galactosidase activity in tissue from Snai2LacZ mice stained in whole mount, sectioned and counterstained with nuclear fast red (D) E14.5midgut, (F) E14.5hind gut, (H) postnatal day 14 SI, (J) postnatal day 14 colon, (K) adult SI. Controls for X‐gal staining are shown as inserts (D', F', H', J').L, M, N Double immunostaining of small intestinalcrypts for Snai1/2 (red) and (M) Lgr5‐GFP (green); (N) merge shows Snai1 is present in both CBCstem cells and proliferating transit amplifying cells. Scale bars: 10 mm.O, P Immunohistochemistry for Snai1 using a different primary antibody (Genetex GTX125918) shows minimal expression of Snai1 in villi. (P) Expression of Snai1 is detected at the base of crypts (P) with strong nuclear expression in CBCstem cells indicated by arrows. Scale bars: 20 mm (O), 10 mm (P).Q Schematic diagram showing Snai1 expression (red) within different cell types in the crypt. SI = small intestine. See also Supplementary Fig S1."
NCBI gene IDs:
"Snai2: 20583"
Figure legend: "Periodic Acid Schiff (PAS) staining was used to detect goblet cells in sections of small intestine from control and Snai1 KO mice. Graph shows number of goblet cells per crypt-villus axes (n = 3).Immunohistochemistry for synaptophysin was used to detect enteroendocrine cells (marked by arrows). Quantification of enteroendocrine cells per crypt-villus axes in control and Snai1 KO tissue revealed a significant increase in the number of enteroendocrine cells following Snai1 loss (n = 3, P = 0.004).Immunohistochemistry for lysozyme was used to detect Paneth cells. Quantification of Paneth cells in control and Snai1 KO indicates a significant increase in Paneth cell numbers following knockout of Snai1 (n = 4, P = 0.002)."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "Measurement of villus length (μm) (n = 3, P = 0.013) and quantification of enterocytes per crypt-villus unit demonstrate a significant reduction in absorptive enterocytes following Snai1 loss (n = 3, P = 0.036).Measurement of villus length (μm) (n = 3, P = 0.003) 8 days after β‐NF induction demonstrates a significant shortening of villi. Bars represent mean ± SD. Two‐tailed Student's t‐test was used to assess significance. Scale bars: 20 μm."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "Sections from control and Snai1 KO small intestine were stained for PCNA to detect proliferating cells (arrows depicting proliferating CBC stem cells at the base of crypts). Quantification of total PCNA‐positive cells per crypt and PCNA‐positive cells in the base of crypts both revealed a significant reduction in the number of proliferating cells after conditional depletion of Snai1 (n = 4, P = 0.049, 0.026). Scale bars: 20 μm."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "The expression of 2 CBCstem cell markers Olfm4 and Lgr5 were examined by in situ hybridisation in control and Snai1KO small intestine. Lgr5 and Olfm4in situ hybridisation staining shows a loss of CBCstem cell marker expression after loss of Snai1 (high magnification inserts depict a representative crypt demarcated by a dotted line). Loss of Snai1 was confirmed in knockout tissue using immunohistochemistry. Scale bars: 20 μm."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "The analysis of sorted and EGFP‐positive cells from Lgr5‐EGFP‐IRES‐creERT2AhCreSnai1fl/fl and Lgr5‐EGFP‐IRES‐creERT2AhCreSnai1+/+mice 5 days after βNF induction show an ˜tenfold reduction in Lgr5GFP‐positive CBCstem cells."
NCBI gene IDs:
"AhCre: 
creERT2: 
IRES: 
Snai1: 20613"
Figure legend: "Immunohistochemistry for active caspase‐3 to detect apoptotic cells revealed a significant increase in the number of apoptotic cells in both crypt (n = 5, P = 0.0001) and villus (n = 5, P = 0.005) regions in Snai1KO tissue (arrows mark active caspase‐3‐positive cells). Scale bars: 20 μm."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "A lineage tracing strategy was employed to determine the fate of CBCcells that lack Snai1. Tissue from Lgr5‐EGFP‐IRES‐creERT2Snai1+/+ (control) and Lgr5‐EGFP‐IRES‐creERT2Snai1fl/fl (Snai1KO) that also contained the ROSA26LacZCre reporter allele was stained with X‐Gal 5 days after tamoxifen injection. Quantification of X‐Gal‐positive foci per cm shows Snai1 knockout tissue failed to lineage trace indicating that no progeny are produced from Snai1 knockout cells. Bars represent mean ± SD (n = 3, P = 0.0001). Scale bars: 0.50 mm. Two‐tailed Student's t‐test was used to assess significance. See also Supplementary Figure S2."
NCBI gene IDs:
"Cre: 
creERT2: 
IRES: 
ROSA26LacZ: 
Snai1: 20613"
Figure legend: "VillinCreERT2Snai1fl/florganoids treated with tamoxifen show arrested growth after 2 and 4 days of treatment compared to controls. Scale bars: 50 μm.Quantification of viable cell growth in VillinCreERT2Snai1fl/florganoids treated with tamoxifen compared to controls over 4 days. Bars represent mean ± SD. n = 3 experiments per group, **P = 0.002."
NCBI gene IDs:
"Cre: 
ERT2: 
Villin: 
Snai1: 20613"
Figure legend: "Assessment of apoptosis in control and VillinCreERT2 Snai1fl/fl organoids after 4 days of growth. Photographs show an overlay of bright‐field and fluorescent images with propidium iodide and Hoechst staining. Quantification of apoptotic organoids. Bars represent mean ± SD. n = 3 experiments per group, **P = 0.00001. Two‐tailed Student's t‐test was used to assess significance. Scale bars: 50 μm. See also Supplementary Figure S3."
NCBI gene IDs:
"Cre: 
ERT2: 
Villin: 
Snai1: 20613"
Figure legend: "The consequences of Snai1 knockout were analysed over a time course. Immunohistochemistry for Snai1 revealed significant loss of expression at day 5 following induction compared to control. The epithelium is re‐populated by wild‐type cells as shown on day 60. Scale bars: 20 μm."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "B Immunohistochemistry for synaptophysin was used to detect enteroendocrine cells. Quantification of the number of enteroendocrine cells per crypt-villus axes revealed a significant decrease in enteroendocrine cell numbers when Snai1 levels are elevated (n = 4, P = 0.003)."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "C Immunohistochemistry for lysozyme was used to detect Paneth cells. Quantification of Paneth cell numbers indicated a significant decrease in Paneth cells in the base of crypts in mice with elevated Snai1 levels (n = 5, P = 0.027)."
NCBI gene IDs:
"Snai1: 20613"
Figure legend: "D, E Quantification of total PCNA‐positive cells per crypt revealed a significant increase when Snai1 levels were elevated (n = 4, P = 0.009). This was confirmed in VillinCreERT2 ROSA26Snai1 mice 5 days after induction with tamoxifen where analysis of (E) PCNA‐positive cells in the base of crypts revealed a significant increase in proliferating cells in crypts with elevated Snai1 levels compared to control tissue (n = 4, P = 0.0005). Bars represent mean ± SD."
NCBI gene IDs:
"Cre: 
ERT2: 
ROSA26: 
Villin: 
Snai1: 20613"
Figure legend: "F PCNAimmunostaining (green) and DAPIstaining (blue) of VillinCreERT2ROSA26Snai1 compared to control VillinCreERT2 littermates. An expansion of proliferating CBCcells (indicated by arrows) is observed at the base of crypts. Scale bars: 10 μm."
NCBI gene IDs:
"Cre: 
ERT2: 
ROSA26: 
Villin: 
Snai1: 20613"
Figure legend: "G qRT-PCR analysis of stem cell marker expression in the small intestine from VillinCreERT2 ROSA26Snai1 compared to control VillinCreER2T littermates. Bars represent mean ± SD. n = 3 experiments per group, Olfm4 P = 0.013, Cdh1/E‐cadherin P = 0.003, Lgr5 P = 0.013."
NCBI gene IDs:
"Cre: 
ERT2: 
ROSA26: 
Villin: 
Cdh1: 12550
E‐cadherin: 12550
Lgr5: 14160
Olfm4: 380924
Snai1: 20613"
Figure legend: "H CD44v6 immunostaining of tissue from AhCre control compared to AhCre RosaSnai1 tissue. A significant increase in CD44v6‐positive cells localised at the base of crypts (depicted by arrows) was observed. Bars represent mean ± SD. n = 3 mice per group, *P = 0.00023. Two‐tailed Student's t‐test was used to assess significance. Scale bars: 10 μm."
NCBI gene IDs:
"AhCre: 
Rosa: 
Snai1: 20613"
Figure legend: "VillinCreERT2 RosaSnai1 organoids treated with tamoxifen show slower growth after 2 and 4 days of treatment compared to controls. Scale bars: 50 μm.Quantification of viable cells in VillinCreERT RosaSnai1 organoids treated with tamoxifen compared to control growth over 4 days. Bars represent mean ± SD. n = 3 experiments per group, **P = 0.002."
NCBI gene IDs:
"Cre: 
ERT2: 
Rosa: 
Villin: 
Snai1: 20613"