EMBO/SourceData huggingface dataset:
Figure legend: "F, G. Consequences of RNF26, UBE2J1, and vimentin loss on induction of PERK-mediated UPR signaling by tunicamycin. F. U2OS cells, transfected or genetically modified as indicated, were treated in the absence (-) or presence (+) of tunicamycin (2.5µg/mL, 5hrs). Immunoblots of whole cell lysates against endogenous PERK, phospho-eIF2a, ATF4, HERP1, and Vinculin (loading control) are shown, representative of three independent experiments. G. CHOP transcripts isolated from U2OS cells transfected or genetically modified as indicated and treated in the absence (-) or presence (+) of tunicamycin (2.5ug/mL, O/N) were detected by qPCR and normalized to GAPDH; n siC (-)=4, n siC (+)=4, n siRNF26#1 (+)=3, n siRNF26#2 (+)=4, n PARENTAL (+)=4, n Vim KO (+)=4 independent experiments. Data information: Graphs report mean or median (red line) of sample values (open circles). Statistical analyses were performed using Students' T-test G error bars indicate mean -/+ SEM) p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001."
NCBI gene IDs:
"CHOP: 1649
PERK: 9451
GAPDH: 2597
RNF26: 79102
UBE2J1: 51465
Vim: 7431
vimentin: 7431"
Figure legend: "A, Analysis of localization of RNF26 sequence variants and HERP1 or HERP2 in the ER. A. Representative confocal images of U2OS cells transfected with GFP-RNF26 WT, RFP-RNF26 I382R, GFP-RNF26 ΔRING and HERP1 or GFP-RNF26 WT with HA-HERP2 that were stained for HA and endogenous VAP-A. Indicated single channels and their corresponding color overlays are shown. Zoom-ins highlight PN regions. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm."
NCBI gene IDs:
"GFP: 
HA: 
RFP: 
HERP1: 9709
HERP2: 64224
RNF26: 79102"
Figure legend: "C. Analysis of determinants for RNF26 complex formation with ERQC proteins HERP1 and HRD1. U2OS cells were transfected with RFP-RNF26 variants (WT, I382R, ΔRING) or EV with either HA-HERP1 or HA-HRD1 before lysis in 1% DMNG detergent, pulldown with RFP-TRAP beads and analysis by SDS-PAGE and WB. Representative images of three independent replicates."
NCBI gene IDs:
"HA: 
HRD1: 
RFP: 
HERP1: 9709
RNF26: 79102"
Figure legend: "D, E. Analysis of localization of ERQC domains with vimentin. D. Representative confocal images of U2OS cells transfected with HA-HERP1 that were stained for endogenous vimentin. Indicated single channels and their corresponding color overlays are shown. Zoom-ins highlight PN regions where vimentin surrounds HERP1-labeled ERQC domains. E. Line graph analysis of HA-HERP1 (magenta) and vimentin (green) signals from E (bottom zoom image). Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm."
NCBI gene IDs:
"HA: 
HERP1: 9709"
Figure legend: "F, G. Analysis of HERP1-induced ERQC domain localization as a function of vimentin and RNF26. F. Representative confocal fluorescence images of control cells, RNF26 KO cells, or vimentin KO#2 cells, transfected with HA-HERP1 and immunostained for HA. Zoom-ins highlight PN regions. G. Perinuclearity analysis of HA-HERP1 in the presence or absence of RNF26 or vimentin. Plot reports median (red line) and 95% confidence interval (error bar) of sample values (open circle), n Parental=79, n R26 KO=51, n Vim KO=45 technical replicates from three independent experiments (Mann-Whitney U test; ** p&lt;0.01, *** p&lt;0.001). Graphs report median (red line) and 95% confidence interval (error bars) of sample values (open circles). Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm."
NCBI gene IDs:
"HA: 
HERP1: 9709
R26: 79102
RNF26: 79102
Vim: 7431
vimentin: 7431"
Figure legend: "C, D. Lysosomal processing of Sec62-HALO-GFP over time in the presence or absence of RNF26 or vimentin. C. Parental, Vim KO#2 or RNF26 KO U2OS cells were transfected with Sec62-HALO-GFP overnight, incubated with 6-chlorohexanol for 15 minutes, washed 3 times with PBS, pulsed with TAMRA HALOtag ligand (200nM) for 1hr, and incubated with 6-chlorohexanol with or without Bafilomycin A1 (100nM) for the indicated times before cell lysis and analysis by SDS-PAGE. Shown is a representative in-gel fluorescence scan with indication of full-length Sec62, protease-resistant HALO fragment and molecular weight markers (MW), as well as a Vinculin blot (loading control) D. Quantification of Sec62 accumulation in LEs from (B). Unprocessed and protease-resistant (lysosomal) band intensities in (B) were calculated with ImageJ and ratios of lysosomal Sec62 versus unprocessed Sec62 were normalized to transfer in parental cells after 10 hours. Error bars show mean +/- SD of n Parental=6, n R26 KO=4, n Vim KO =6 independent experiments. Scale bar = 10µm. Graphs report mean (in D of sample values (open circles). Statistical analyses were performed using Students' T-test (D (paired) ; * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001)."
NCBI gene IDs:
"R26: 79102
RNF26: 79102
Vim: 7431
vimentin: 7431"
Figure legend: "E, F. Sec62-mediated transfer of ER to lysosomes as a function of RNF26 or vimentin. E. Representative Airyscan images of Parental, RNF26 KO or Vim KO#2 U2OS cells that were transfected with Sec62-HALO-GFP, incubated with 6-chlorohexanol for 15 minutes, washed 3 times with PBS, pulsed with JF646 HALOtag ligand for 1hr, and incubated with 6-chlorohexanol and Bafilomycin A1 for 8hrs before fixation and immunostaining for CD63 to visualize LEs. F. Colocalization (Manders') analysis of CD63 over HALO signals from (D). Plot reports n Parental=106, n R26 KO=69, n Vim KO =68 technical replicates from three independent experiments. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, respectively. Scale bar = 10µm. Graphs report mean (in F) of sample values (open circles). Statistical analyses were performed using Students' T-test F (unpaired) ; * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001)."
NCBI gene IDs:
"GFP: 
HALO: 
R26: 79102
RNF26: 79102
Sec62: 7095
Vim: 7431
vimentin: 7431"
Figure legend: "G, H. Sec62-mediated transfer of ER to lysosomes as a function of RNF26 overexpression and activity. G. Representative spinning disc live cell images of RNF26 WT and I382R inactive mutant with Sec62. U2OS cells were transfected overnight with Sec62-HALO-GFP and RFP-RNF26 WT or I382R, incubated for 5hrs with JF646 and imaged by spinning disc microscopy. Images show overlay of GFP (green), JF646 (magenta) and RFP signals, zoom-ins of overlay and single channels that show transfer of RNF26 into LEs (WT RNF26). H. Analysis of Sec62 transfer to proteolytic compartments as a function of RNF26 WT or I382R overexpression. Number of GFP-negative/HALO-positive signals was manually counted from cells in (B) and (G). Plot reports n EV=86, n R26 WT=35, n R26 IR =40 technical replicates from three independent experiments. Data information: Cell and nuclear boundaries are demarcated using dashed and continuous lines, median (in H) of sample values (open circles). Mann-Whitney U test (H); * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001)."
NCBI gene IDs:
"GFP: 
HALO: 
RFP: 
R26: 79102
RNF26: 79102
Sec62: 7095"
Figure legend: "ALP assay. The cells expressing Pho8Δ60p were cultured in SD+CA medium (open bars) and shifted to SD(−N) medium for three hours (filled bars). The ALP activity of the lysates was measured to estimate autophagic activity. Wild-type cells, TN124; Δapg8 mutant harboring vector, TK303; Δapg8 mutant harboring APG8 on a centromeric vector, TK301; Δapg8 mutant harboring 3 × HA-tagged APG8 plasmid, TK307."
NCBI gene IDs:
"apg8: 852200
APG8: 852200
Pho8: 852092"
Figure legend: "Expression of APG8. A, The lysates prepared from the growing cells were subjected to immunoblotting with the anti-Apg8p antibody. Lane 1, wild-type cells (YW5-1B); lane 2, Δapg8 cells harboring APG8 on a multicopy vector (TK201); lane 3, Δapg8 cells (TK404)."
NCBI gene IDs:
"apg8: 852200
APG8: 852200"
Figure legend: "C, YW5-1B cells were cultured in YEPD medium until 1-2 × 107 cells/ml (0 h) and shifted to SD(−N) medium for 0.5, 1, 2, and 6 h at 30°C, and mRNA was prepared from each culture as described in Materials and Methods. APG8 mRNA and ACT1 mRNA were detected by Northern blotting with each specific probe. Each lane has 4 μg of total mRNA."
NCBI gene IDs:
"ACT1: 
APG8: 852200"
Figure legend: "Immunofluorescent staining of Δapg8 cells expressing 3 × HA-Apg8p. A, The Δapg8 cells harboring 3 × HA-tagged APG8 plasmid, TK114 cells, were grown until logarithmic phase. The cells were fixed by formaldehyde and were treated with Zymolyase 100T to generate spheroplasts. The spheroplasts were permeabilized by 0.5% Triton X-100, and incubated with the anti-HA antibody, 16B12, followed with the FITC-conjugated anti-mouse IgG. Left, Fluorescence image of 3 × HA-Apg8p; right, Nomarski image of the cells. Arrows show punctate signals that are a little larger than tiny dot signals. Bars, 10 μm. B, Immunofluorescent staining of YW5-1B cells before (0 h) and after (3 h) shift to starvation (negative control). Left, Fluorescence image; right, Nomarski image. Bars, 10 μm. C, The cells were shifted to starvation for 0, 0.5, and 3 h. Upper panels, Fluorescence images of 3 × HA-Apg8p; lower panels, Nomarski images of the cell. Bars, 5 μm."
NCBI gene IDs:
"apg8: 852200
APG8: 852200"
Figure legend: "Change of Apg8p localization in Δpep4 cells after shift to starvation. The Δapg8Δpep4 cells harboring 3 × HA-tagged APG8 plasmid, TK116 cells, were shifted to starvation for 0, 0.5, 1, 3, and 6 h. Immunofluorescence microscopy was performed as described in Fig. 5. A-E, FITC staining of 3 × HA-Apg8p. F-J, Nomarski images of the cells. Arrows show an autophagic body. Bars, 5 μm."
NCBI gene IDs:
"apg8: 852200
APG8: 852200
pep4: 855949"
Figure legend: "Immunostaining image of a cell containing autophagic bodies in the vacuole. The Δapg8Δpep4 cells expressing 3 × HA-Apg8p, TK116 cells, were shifted to SD(−N) medium for two hours. Cells were immunolabeled with anti-N mAb, 16B12, followed by 10-nm gold-conjugated goat anti-mouse IgG. AB, vacuole; N, nucleus; V, vacuole."
NCBI gene IDs:
"apg8: 852200
pep4: 855949"
Figure legend: "Fine morphology of Δypt7 and Δapg8 cells and proteinase-K accession to proAPI in these mutants. A, EM image of Δypt7 cell starved in SD(−N) medium for four hours. Autophagosomes (AP, arrows) were accumulated. B, EM image of the starved Δapg8 cell. C-E, The representatives of the membrane structures detected in the starved Δapg8 cells. C, Autophagosome-like structure indistinguishable from autophagosome. D and E, Aberrant multivesicular structures. N, nucleus; V, vacuole. F, Proteinase K-sensitivity of proAPI. Cell lysates were prepared from Δypt7 and Δapg8 cells starved in SD(−N) medium for 4.5 h. The lysates were treated with 100 μg/ml proteinase K in the presence or absence of Triton X-100. Asterisk shows mature API and digestion product of proAPI by proteinase K."
NCBI gene IDs:
"apg8: 852200
ypt7: 855012"
Figure legend: "Colorectal tumor outcomes in WT (n=7), IL-33KO (n=4) and ST2KO (n=3) mice at the completion of the AOM/DSS carcinogenesis protocol. (A) Tumor volumes at the endpoint (each dot represents a tumor, mice with no tumor are marked as zero), Skin tumor outcomes in WT (n=7), IL-33KO (n=6) and ST2KO (n=7) mice at the completion of the DMBA/DNFB carcinogenesis protocol. (D) Tumor volumes at the endpoint (each dot represents a tumor, mice with no tumor are marked as zero),"
NCBI gene IDs:
"IL-33: 77125
ST2: 17083"
Figure legend: "H) Quantification of nuclear IL-33+ epidermal and dermal cells in the DNFB- versus acetone-treated WT skin. Dots represent cell counts from three randomly selected high power field (HPF) images per sample (n=5 per group). J) Quantification of nuclear IL-33+ epithelial and stromal cells of colon treated with AOM/DSS versus no treatment. Dots represent cell counts from three randomly selected HPF images per sample (n=4 per group). Data information: Graphs show mean + SD, unpaired t-test. "
NCBI gene IDs:
"IL-33: 77125"
Figure legend: "(H) ChIP-qPCR assay for Smad6 in the presence of IL-33 full length or cytokine domain using an anti-RUNX2 antibody. After Pam212 cells were transfected with IL-33 full length or cytokine domain for 24 hours, cell lysates were subjected to chromatin-immunoprecipitation with anti-RUNX2 antibody and eluted RUNX2-bound chromatin was used for qPCR with primers against Smad6 promoter region. Anti-IgG ChIP-qPCR results are shown as negative controls (n=4 per group)."
NCBI gene IDs:
"IL-33: 77125
Smad6: 17130"
Figure legend: "(D) Immunoblot of p-SMAD2/3 and SMAD2/3 proteins in response to TGF-β and poly (I:C) treatment. Pam212 cells were treated with poly (I:C) or PBS followed by incubation with and without TGF-β (5nM). Data represent three independent experiments with similar results. (E) Immunoblot of p-SMAD2/3 and SMAD2/3 proteins in response to TGF-β + poly (I:C) after knocking down of IL-33. Pam212 cells were transfected with siIl33 knockdown construct or siRNA control (siCon) for 30 hours followed by incubation with poly (I:C) and TGF-β. Data represent three independent experiments with similar results. (F) Immunoblot of p-SMAD2/3 and SMAD2/3 proteins upon the expression of IL-33 full length or cytokine domain. Pam212 cells were transfected with IL-33 full length or cytokine domain for 24 hours followed by incubation with and without TGF-β. Data represent three independent experiments with similar results. Data information: GAPDH is used as the control housekeeping protein in D-F). Graphs show mean + SD, NS: not significant, unpaired t-test."
NCBI gene IDs:
"IL-33: 77125
siIl33: 77125"
Figure legend: "(G) Impact of IL-33 knockdown on cell proliferation in response to TGF-β. Pam212 cells were treated with siIl33 or siCon followed by TGF-β treatment (n=7 in each group). (H) Impact of IL-33 knockdown and SB431542 treatment on cell proliferation. Pam212 cells were treated with siIl33 or siCon in combination with SB431542 or DMSO (carrier control, n=7 in each group). "
NCBI gene IDs:
"IL-33: 77125
siIl33: 77125"
Figure legend: "(I) SMAD signaling proteins levels in WT and K14-IL33tg,ST2KO skin treated with acetone (control) at the completion of the DMBA/TPA carcinogenesis protocol. Tissue lysates prepared from the whole back skin of the animals were subjected to immunoblot with p-SMAD2/3, p-SMAD1/5, SMAD6, SMAD2/3 and SMAD1 antibodies. GAPDH is used as the control housekeeping protein (n=5 in each group)."
NCBI gene IDs:
"IL33: 77125
K14: 3861
ST2: 17083"
Figure legend: "(B) Il33 and Smad6 mRNA levels in caerulein-treated WT pancreas compared with PBS-treated controls at the completion of chronic pancreatitis protocol (n=8 in caerulein and n=9 in PBS group for Il33, n=9 in caerulein and n=10 in PBS group for Smad6, unpaired t-test)."
NCBI gene IDs:
"Il33: 77125
Smad6: 17130"
Figure legend: "(D) Pancreatic tumor-free survival of WT (n=4), IL-33KO (n=5) and ST2KO (n=6) mice at the completion of the DMBA/caerulein carcinogenesis protocol (log-rank test)."
NCBI gene IDs:
"IL-33: 77125
ST2: 17083"
Figure legend: "B Mitochondrial transcript levels measured by qRT-PCR in RKO cells after 96 hours of 1 μM  IMT1 treatment. Data are relative to  DMSO-treated controls and are expressed as mean ± SD of n=4 independent experiments. Ordinary one-way ANOVA was used for comparisons to DMSO-treated controls (RNR1, RNR2,  MT-COX2, MT-ATP6, MT-ND1: ***p&lt;0,0001)."
NCBI gene IDs:
"MT-ATP6: 4508
MT-COX2: 4513
MT-ND1: 4535
RNR1: 6052
RNR2: 4550"
Figure legend: "G Relative mtDNA copy number measured in RKO and  IMT1-resistant cells treated with  IMT1  for 96 hours. Data are expressed as average from n=3 experiments ± SEM. Statistical significance was calculated with one-way ANOVA test.  RKO + IMT1 vs Resistant + IMT1: MT-ND1 **p=0,0035, MT-ATP6 ***p=0,0002, MT-CYTB **p=0,0028."
NCBI gene IDs:
"MT-ATP6: 4508
MT-CYTB: 4519
MT-ND1: 4535"
Figure legend: "G Viable cells counts of IMT1-resistant RKO cells treated for one-week with either DMSO or IMT1 in presence of controls (control #1, #2) or TFAM (TFAM  #1, TFAM #2) siRNAs. Data are expressed as mean values ± SD of n=5 independent experiments each including six technical intra-plate replicates. Statistical significance was calculated with one-way ANOVA test. Controls + IMT1 vs TFAM #1 + IMT1: p= 0,2148; Controls + IMT1  vs TFAM #2 + IMT1: *p=0,0415."
NCBI gene IDs:
"TFAM: 7019"
Figure legend: "293T cells were transfected with a plasmid encoding FLAG-Stx17 wild type (WT) or the indicated constructs. At 24 h after transfection, cell lysates were immunoprecipitated (IP) with anti-FLAG M2 beads, and analyzed by IB using antibodies against PGAM5 and FLAG. Five percent of lysates was analyzed as input."
NCBI gene IDs:
"FLAG: 
Stx17: 55014"
Figure legend: "HeLa cells stably expressing FLAG-Stx17 WT or the K254C mutant were fixed and subjected to PLA using antibodies against FLAG and PGAM5. Scale bar, 5 μm. Values are means ± SEM (n = 3). ***P&lt;0.001 as compared with WT (paired Student's t-test)."
NCBI gene IDs:
"FLAG: 
Stx17: 55014"
Figure legend: "MBP or the MBP-Stx17 constructs attached to amylose resin were mixed with GST-PGAM5, and the proteins bound to the resin were separated by SDS-PAGE and blotted onto PVDF membranes. The blots were detected by an anti-GST antibody (upper panels) or stained with Coomassie Brilliant Blue R-250 (lower panels). Ten percent of the proteins used for each experiment was analyzed as input. Asterisks and double asterisk may represent MBP dimers and degradation products."
NCBI gene IDs:
"GST: 
MBP: 
PGAM5: 192111
Stx17: 55014"
Figure legend: "293T cells were cotransfected with plasmids encoding FLAG-Stx17 WT and the indicated PGAM5-GFP constructs, and analyzed"
NCBI gene IDs:
"FLAG: 
GFP: 
PGAM5: 192111
Stx17: 55014"
Figure legend: "293T cells were cotransfected with plasmids encoding FLAG-Stx17 or Stx18 and the C-terminally GFP-tagged transmembrane domain of PGAM5（amino acids 1-35）constructs, and analyzed"
NCBI gene IDs:
"FLAG: 
GFP: 
Stx18: 
PGAM5: 192111
Stx17: 55014"
Figure legend: "HeLa cells stably expressing FLAG-Stx17 wild type (WT) were transfected with a plasmid encoding Su9-GFP (mitochondria) or Sec61β-GFP (ER). At 24 h after transfection, the cells were subjected to PLA using antibodies against FLAG and PGAM5. Scale bar, 5 μm. The bar graph on the right shows the Manders' coefficients for the colocalization of PLA dots and Su9-GFP or Sec61β-GFP. Values are means ± SEM (n = 3). ***P&lt;0.001 (paired Student's t-test)."
NCBI gene IDs:
"FLAG: 
GFP: 
Sec61β: 
Su9: 
Stx17: 55014"
Figure legend: "HeLa cells stably expressing FLAG-Stx17 WT were mock-transfected or transfected with siRNA for Mfn1, Mfn2 or PACS-2. At 72 h after transfection, the cells were subjected to PLA using antibodies against FLAG and PGAM5. Scale bar, 5 μm. Values are means ± SEM (n = 3). ***P&lt;0.001 as compared with Mock (paired Student's t-test)."
NCBI gene IDs:
"FLAG: 
Mfn1: 55669
Mfn2: 9927
PACS-2: 23241
Stx17: 55014"
Figure legend: "HeLa cells were mock-transfected or transfected with siRNA for Mfn1, Stx17, Mfn2 or PACS-2. At 72 h after transfection, the cells were subjected to PLA using antibodies against Drp1 and PGAM5. Scale bar, 5 μm. Values are means ± SEM (n = 3). ***P&lt;0.001 as compared with Mock (paired Student's t-test)."
NCBI gene IDs:
"Mfn1: 55669
Mfn2: 9927
PACS-2: 23241
Stx17: 55014"
Figure legend: "HeLa cells with mock treatment (Mock) or depleted of Stx17 (Stx17 KD) were fixed and double immunostained for PGAM5 and Tom20. Scale bar, 5 μm."
NCBI gene IDs:
"Stx17: 55014"
Figure legend: "293T cells with mock treatment or depleted of Stx17 were incubated with ethanol (Vehicle) or 20 μM CCCP (+CCCP) for 2 h, lysed and then analyzed by IB using the indicated antibodies."
NCBI gene IDs:
"Stx17: 55014"
Figure legend: "293T cells transiently expressing FLAG-Stx17 wild type (WT) or the K254C mutant were incubated with ethanol (Vehicle) or 20 μM CCCP (+CCCP) for 2 h, lysed, immunoprecipitated with anti-FLAG M2 beads and then analyzed by IB using the indicated antibodies."
NCBI gene IDs:
"FLAG: 
Stx17: 55014"
Figure legend: "HeLa cells stably expressing FLAG-Stx17 WT were incubated with ethanol (Vehicle) or 20 μM CCCP (+CCCP) for 2 h, and subjected to PLA using antibodies against FLAG and PGAM5. Scale bar, 5 μm. Values are means ± SEM (n = 3). ***P&lt;0.001 as compared with Vehicle (paired Student's t-test)."
NCBI gene IDs:
"FLAG: 
Stx17: 55014"
Figure legend: "HeLa cells stably expressing GFP-Parkin with mock treatment (Control) or depleted (KD) of Stx17, PGAM5 or Drp1 were incubated for 16 h with ethanol (Vehicle) or 10 μM CCCP (+CCCP), and analyzed by IB using the indicated antibodies."
NCBI gene IDs:
"GFP: 
Drp1: 10059
PGAM5: 192111
Parkin: 5071
Stx17: 55014"
Figure legend: "HeLa cells stably expressing GFP-Parkin with mock treatment (Mock) or depleted of Stx17, PGAM5 or Drp1 were incubated in the absence (Vehicle) or presence of 20 μM CCCP (+CCCP) for 2 h and analyzed by immunofluorescence microscopy. Scale bar, 5 μm. The bar graph on the right shows the Manders' coefficients for the colocalization of GFP-Parkin and Tom20. Values are means ± SEM (n = 3). ***P&lt;0.001 as compared with Mock (paired Student's t-test)."
NCBI gene IDs:
"GFP: 
Drp1: 10059
PGAM5: 192111
Parkin: 5071
Stx17: 55014"
Figure legend: "PINK1-FLAG and GFP-Parkin stably expressing HeLa cells with mock treatment or depleted of Stx17 or PGAM5 were incubated in the absence (ethanol) or presence of 20 μM CCCP for 2 h and analyzed by IB using the indicated antibodies. F, full-length PINK1; C, cleaved PINK1."
NCBI gene IDs:
"FLAG: 
GFP: 
PGAM5: 192111
PINK1: 65018
Parkin: 5071
Stx17: 55014"
Figure legend: "GFP-Parkin stably expressing HeLa cells were mock-transfected (Mock) or transfected with siRNA (KD) for Stx17 or PGAM5. At 48 h after siRNA transfection, the cells were transfected with a plasmid encoding FLAG-DFCP1, incubated for 24 h, treated with 20 μM CCCP for 2 h and then immunostained for FLAG. Scale bars, 5 μm. The bar graph below shows the Manders' coefficients for the colocalization of GFP-Parkin and FLAG-DFCP1. Values are means ± SEM (n = 3). **P&lt;0.01 as compared with Mock (paired Student's t-test)."
NCBI gene IDs:
"DFCP1: 
FLAG: 
GFP: 
PGAM5: 192111
Parkin: 5071
Stx17: 55014"
Figure legend: "GFP-Parkin stably expressing HeLa cells with mock treatment or depleted of Stx17 or PGAM5 were incubated with 20 μM CCCP for 2 h and immunostained for LC3. Scale bar, 5 μm. The bar graph below shows the Manders' coefficients for the colocalization of GFP-Parkin and LC3. Values are means ± SEM (n = 3). **P&lt;0.01 as compared with Mock (paired Student's t-test)."
NCBI gene IDs:
"GFP: 
PGAM5: 192111
Parkin: 5071
Stx17: 55014"
Figure legend: "GFP-Parkin stably expressing HeLa cells with mock treatment or depleted of Stx17 or PGAM5 were incubated in the absence (Vehicle) or presence of 20 μM CCCP (+CCCP) for 2 h and analyzed by IB using the indicated antibodies."
NCBI gene IDs:
"GFP: 
PGAM5: 192111
Parkin: 5071
Stx17: 55014"
Figure legend: "HeLa cells stably expressing GFP-Parkin with mock treatment (Control) or depleted of FUNDC1 (FUNDC1 KD) were incubated for 16 h with ethanol (Vehicle) or 10 μM CCCP (+CCCP), and analyzed by IB using the indicated antibodies."
NCBI gene IDs:
"GFP: 
FUNDC1: 139341
Parkin: 5071"
Figure legend: "GFP-Parkin stably expressing HeLa cells with mock treatment (Mock) or depleted of FUNDC1 (FUNDC1 KD) were incubated with 20 μM CCCP for 2 h and immunostained for Tom20. Scale bar, 5 μm."
NCBI gene IDs:
"GFP: 
FUNDC1: 139341
Parkin: 5071"
Figure legend: "293T cells with mock treatment (Mock) or depleted of Stx17 (Stx17 KD) were incubated in the absence (Vehicle) or presence of 20 μM CCCP (+CCCP) for 2 h, immunoprecipitated with anti-FLAG M2 beads and then analyzed by IB using the indicated antibodies. Three percent of lysates was analyzed as input. During Stx17 knockdown, cells were transfected with a plasmid encoding FLAG-FUNDC1."
NCBI gene IDs:
"FLAG: 
FUNDC1: 139341
Stx17: 55014"