EMBO/SourceData huggingface dataset:
Figure legend: "(A) The formation of secondary ParBF-DNA complexes requires the box II motif. EMSA were performed with a 144-bp 32P-labelled DNA fragments (C144) carrying a single 16-bp parS binding motif. Reaction mixtures containing 100 µg.ml-1 sonicated salmon sperm DNA were incubated in the absence (-) or the presence of increasing concentrations (grey triangle; 10, 30, 100, 300, and 1000 nM) of ParBF or ParBF-3R*. Positions of free and bound probes are indicated on the left. B1 represents complexes involving the specific interaction on the 16-bp binding site, while B\"2 and B\"3 complexes represent secondary complexes involving the parSF site with one or two additional nsDNA-binding interactions, respectively (Sanchez et al,"
NCBI gene IDs:
"parS: 
parSF: "
Figure legend: "(C) ParBF in vivo DNA binding in the vicinity of parSF sites requires the box II motif. ChIP-seq was performed on DLT3726 carrying parSF in the xylE chromosomal locus and expressing ParBF-3R* variant. ParBF-3R* DNA binding profile displayed the number of nucleotide reads as a function of the E. coli genomic coordinates. The peak at parSF covered approximately 950-bp, which corresponds to the 402-bp between the 1st and 10th specific binding sites and ~280-bp on each sides (representing the average size of the DNA library; see Appendix Fig. S3E). No ParBF-3R* enrichment was found on parSF-flanking DNA and elsewhere on the chromosome. Inset, zoom in on the right side of parSF over 5-Kbp with the ParB density, normalized to 1 at the first bp after the last parS binding repeat, plotted as a function of the distance from parSF. Note that a highly similar DNA binding pattern is obtained with ParBF -3R*-mVenus (strain DLT3566; Appendix Fig. S3C)"
NCBI gene IDs:
"parS: 
parSF: 
xylE: 948529"
Figure legend: "(A) Schematic representation of the genomic locus of the chromosome 1 of V. cholerae with the three parS sites, named parS1-3. The rRNA operon (blue rectangle) spans the genomic coordinates 53823 to 59123 (B) ChIP-seq performed on strain N16961 is displayed as the number of nucleotide reads in function of the genomic coordinates. Correspondence to the parS1-3 location represented in (A) is indicated by grey dotted lines. The number of reads in the Input sample (grey line) is normalized to the total number of reads in the IP sample. (C) We modeled the ChIP-Seq data as in Fig.1C-E by means of MC simulations with a Freely jointed chain of size N=2000 monomers of size a=16-bp. Data are normalized after background subtraction to the read value at parS1 (genomic coordinate 62438). The best fit was achieved with σ=25nm and an amplitude κ=0.15 leading to Nt~50 ParB on the chromosome. In the MC simulation, we accounted for the finite width of the distribution around parS sites by including the average fragment size of the DNA librar"
NCBI gene IDs:
"parS: "
Figure legend: "ChIP-sequencing assays were performed on DLT2075 (xylE::parSF) expressing ParBF grown in exponential (expo) or stationary (stat) phases with addition of rifampicin when indicated (+Rif). The assays have been performed in duplicate for the +Rif and once for the stationary phase experiments. (A) ParBF DNA binding around parSF is independent of active transcription. The color-coded ParBF profiles are represented over 50-Kbp as the relative ParB density normalized to 1 at the first bp after the last parSF binding site. Loci A, C, E and F are defined in Appendix Fig. S1A. (B) The dips and peaks are highly similar in the three indicated conditions. Same as in (A) with zoom in on the right side of parSF up to 9-Kpb and normalization to 1 at genomic coordinate 230. The dotted line corresponds to the analytics description of &quot;Nucleation and caging&quot; (see details in Fig. 1C-E). (C) ParBF binding profile upstream of the locus A. Same as in (A) with zoom in from -6.5 to -16.5-Kbp by normalization to 1 at genomic coordinate -6.5-Kbp (upstream of the dip at the locus A). The ParBF DNA binding profile remains compatible to a power-law, represented by the analytics description (dotted line), upstream of the locus A in stationary phase (black) and in exponential phase (blue). Also, the dips and peaks are highly similar in both conditions. These data are not in favor of the &quot;1D-spreading&quot; or the &quot;Spreading and bridging&quot; models that predicts a basal uniform distribution or a linear decrease after a barrier, respectively (Broedersz et al, 2014). (D) The promoter region at locus A prevents ParBF DNA binding. Chip-seq assays were performed in isogenic xylE::parSF strains (DLT2075; black curve) in which the locus A is replaced by a kanamycin gene (DLT3651; red curve). The assay in the ∆(locus A) genomic context has been performed once. The relative ParB density as a function of the distance from parSF is"
NCBI gene IDs:
"parSF: 
xylE: 948529"
Figure legend: "(a) Densitometric analysis relative to actin of A53T α-synuclein clearance in stable inducible PC12 cell line expressing A53T α-synuclein. Transgene expression was induced with doxycycline for 48 h, and then switched off (by removing doxycycline), with drug (all 1 μM) or DMSO (control) treatment for 24 h. Control condition is set to 100%. Error bars show s.e.m."
NCBI gene IDs:
"α-synuclein: 6622"
Figure legend: "(e) The proportions of EGFP-positive cells with EGFP-HDQ74 aggregates in wild-type (Atg5+/+) and knockout (Atg5−/−) Atg5 MEFs, transfected with EGFP-HDQ74 for 4 h and then treated for 48 h with drugs (all 1 μM) or DMSO (control). Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05; NS, nonsignificant."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(a) A53T α-synuclein clearance in stable PC12 cells as in Figure 1a, treated with or without 1 μM rilmenidine, 1 mM dibutyl-cAMP (db-cAMP), 24 μM forskolin or 500 μM 2′5′-dideoxyadenosine (2′5′ddA) for 24 h. Note that control clearance levels for a given substrate may vary from figure to figure, due to different exposures of blots, which aid illustration of agents that either increase or retard clearance."
NCBI gene IDs:
"α-synuclein: 6622"
Figure legend: "(f) COS-7 cells, transfected for 4 h with EGFP-HDQ74 along with empty vector (pcDNA3.1), dominant-negative S17N Rap2B (DN Rap2B) or wild-type PLC-ε (1:3 ratio), were assessed for the proportions of EGFP-positive cells with aggregates after 48 h. Error bars show 95% confidence interval."
NCBI gene IDs:
"PLC-ε: 51196
Rap2B: 5912"
Figure legend: "(g) Endogenous LC3-II levels in COS-7 cells transfected with a dominant-negative Rap2B (DN Rap2B) for 4 h were assessed after 48 h expression."
NCBI gene IDs:
"Rap2B: 5912"
Figure legend: "(h) The proportions of EGFP-positive COS-7 cells with aggregates, transfected with EGFP-HDQ74 along with empty vector (pcDNA3.1) or dominant-negative Rap2B (1:3 ratio) for 4 h, were treated with or without 10 μM 8-CPT-2Me-cAMP for 48 h. Error bars show 95% confidence interval."
NCBI gene IDs:
"Rap2B: 5912"
Figure legend: "(i) COS-7 cells, transfected with EGFP-HDQ74 and dsRed2 or cytosolic dsRed2-IP3 kinase A (1:3 ratio) for 4 h, were assessed at 48 h post-transfection for aggregation and cell death (as in Fig. 1c). Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05; NS, nonsignificant."
NCBI gene IDs:
"IP3 kinase A: 3706"
Figure legend: "(e) COS-7 cells transfected with empty vector (pcDNA3.1) or wild-type PLC-ε for 4 h and treated with or without 50 μM calpeptin for 48 h post-transfection were assessed for the proportion of EGFP-positive cells with EGFP-HDQ74 aggregates. Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05; NS, nonsignificant."
NCBI gene IDs:
"PLC-ε: 51196"
Figure legend: "(b) HeLa cells were transfected with EGFP-HDQ74 construct along with siGLO (control siRNA) in presence or absence of calpain 1 or calpain 2 siRNA (1:3 ratio) for 96 h. EGFP- and siGLO-positive cells were assessed for aggregation and cell death as in Figure 1c. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain 1: 823
calpain 2: 824"
Figure legend: "(c) HeLa cells, transfected with siGLO or siRNAs for calpain 1 or calpain 2 for 96 h, were analyzed with anti-calpain 1 or anti-calpain 2 domain III antibodies."
NCBI gene IDs:
"calpain 1: 823
calpain 2: 824"
Figure legend: "(f) COS-7 cells, transfected with EGFP-HDQ74 along with empty vector (pcDNA3.1) or constitutive active (CA) m-calpain (1:3 ratio) for 48 h, were assessed for aggregation and cell death as in Figure 1c. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(g) SK-N-SH cells, transfected with EGFP-HDQ74 along with empty vector (pcDNA3.1) or constitutive active m-calpain (1:3 ratio) for 4 h, were subsequently treated with DMSO (control), 1 μM verapamil or 1 μM clonidine for 48 h. The proportions of EGFP-positive cells with aggregates were assessed as in Figure 1c. Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(a) The proportions of EGFP-positive MEFs with aggregates, transfected with EGFP-HDQ74 and pcDNA3.1 (empty vector) in Atg5+/+ cells, or transfected with EGFP-HDQ74 and pcDNA3.1, WT Atg5 or K130R Atg5 constructs in Atg5-/- cells for 4 h (1:3 ratios), were treated for next 48 h with or without 10 μM calpastatin. All data are from the same experiment, but odds ratios for reference conditions in specific experiments have been set to 1, to facilitate comparisons. Atg5-/- cells had increased EGFP-HDQ74 aggregates compared to Atg5+/+ cells. Error bars show 95% confidence interval."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(b) HeLa cells were transfected with siGLO (control) with or without calpain 1 siRNA or calpain 2 siRNA (1:3 ratio) for 96 h, followed by transfection with EGFP-LC3 construct for 4 h. Cells were fixed after a further 2 h. The proportions of transfected cells with &gt;5 EGFP-LC3 vesicles were assessed. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain 1: 823
calpain 2: 824"
Figure legend: "(e) COS-7 cells, transfected with myc-LC3 and EGFP-C1 with pcDNA3.1 (empty vector) or constitutively active (CA) m-calpain (1:1:3 ratio) for 4 h, were analyzed after 24 h post-transfection for LC3-II levels by immunoblotting with anti-myc antibody. Error bars show s.e.m."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(f) COS-7 cells, transfected with EGFP-LC3 and pcDNA3.1 (empty vector) or CA m-calpain (1:3 ratio) for 4 h and fixed 24 h post-transfection, were assessed for proportions of EGFP-positive cells with &gt;5 EGFP-LC3 vesicles. Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05; NS, nonsignificant."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(c) Aggregation in COS-7 cells transfected with either pcDNA3:1 (empty vector) or constitutively active (CA) m-calpain and EGFP-HDQ74 (3:1 ratio) for 4 h and then treated with or without 500 μM 2′5′ddA for 48 h. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(d) HeLa cells transfected with control or Gsα siRNA for 72 h were analyzed for Gsα levels by immunoblotting with anti-Gsα antibody. Aggregation was assessed in HeLa cells transfected with control or Gsα siRNA and EGFP-HDQ74 for 72 h. Error bars show 95% confidence interval."
NCBI gene IDs:
"Gsα: 2778"
Figure legend: "(e) Endogenous LC3-II levels in HeLa cells transfected with control or Gsα siRNA for 72 h."
NCBI gene IDs:
"Gsα: 2778"
Figure legend: "(g) Endogenous LC3-II levels in HeLa cells transfected with control or Gsα siRNA for 72 h and treated with or without 400 nM bafilomycin A1 for the last 4 h. Densitometric analysis is relative to actin. Error bars show s.e.m."
NCBI gene IDs:
"Gsα: 2778"
Figure legend: "(h) The proportions of EGFP-positive HeLa cells with aggregates, transfected with control or Gsα siRNA for 48 h, and then retransfected together with pcDNA 3.1 (empty vector) or CA m-calpain and EGFP-HDQ74 (3:1 ratio) for a further 48 h. Error bars show 95% confidence interval. ***P 0.001; **P 0.01; *P 0.05; NS, nonsignificant."
NCBI gene IDs:
"calpain: 825///824///823
Gsα: 2778"
Figure legend: "(a) The proportions of EGFP-positive COS-7 cells with aggregates or cell death, after transfection with EGFP-HDQ74 and rheb or pcDNA3.1 (empty vector) (1:3 ratio) for 4 h and treatment with or without 10 μM calpastatin or 50 μM calpeptin for 48 h post-transfection. The control condition for assessing the effect of calpain inhibitors in rheb-transfected cells was taken as 1. Error bars show 95% confidence interval."
NCBI gene IDs:
"rheb: 6009"
Figure legend: "(b) The proportions of EGFP-positive COS-7 cells with &gt;5 EGFP-LC3 vesicles, transfected with EGFP-LC3 and pcDNA3.1 (empty vector) or rheb (1:3 ratio) for 4 h and treated with or without 10 μM calpastatin or 50 μM calpeptin for 24 h post-transfection. Error bars show 95% confidence interval."
NCBI gene IDs:
"rheb: 6009"
Figure legend: "(c) The proportions of EGFP-positive COS-7 cells with aggregates, transfected with EGFP-HDQ74 and constitutively active (CA) m-calpain or pcDNA3.1 (empty vector) (1:3 ratio) for 4 h and treated with or without 0.2 μM rapamycin for 48 h post-transfection. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "(d) The proportions of EGFP-positive COS-7 cells with &gt;5 EGFP-LC3 vesicles, transfected with EGFP-LC3 and CA m-calpain or pcDNA3.1 (empty vector) (1:3 ratio) for 4 h and treated with or without 0.2 μM rapamycin for 24 h post-transfection. Error bars show 95% confidence interval."
NCBI gene IDs:
"calpain: 825///824///823"
Figure legend: "A. PCR analysis of the human H49K variant of the ATPIF1 and rtTA constructs in wild-type (wt), ACTA1-rtTA (T), ATPIF1H49K-TRE (H) or double transgenic (T/H) mice."
NCBI gene IDs:
"ACTA1: 58
ATPIF1: 93974"
Figure legend: "(B WB expression of the human (h) ATPIF1 protein in Skm (B extracts. hATPIF1 is only expressed in Skm from ATPIF1H49K|T/H mice. HSP60 are shown as loading controls. n= 3 mice/genotype."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "(C) Immunofluorescent staining of wt and ATPIF1H49K|T/H hindlimb muscle slices with hATPIF1 and βF1 antibodies. Images are representative of 3 mice/genotype, 5 fields/mouse. hATPIF1 H49K is only expressed in T/H mice."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "D) WB expression of the human + mouse (m+h) ATPIF1 protein in Skm D), brain, liver, and WAT (D) extracts. hATPIF1 is only expressed in Skm from ATPIF1H49K|T/H mice. α-tubulin are shown as loading controls. n= 3 mice/genotype."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "E. Polarographic profiles of isolated mitochondria from wt (lower trace) and ATPIF1H49K|T/H (upper trace) animals. Histograms show a reduction in state 3 respiration consistent with the inhibition of ATP synthase in ATPIF1H49K|T/H mice. Bars are the mean ± s.e.m. of n= 3 mice/genotype, 3 traces/mouse; OL, oligomycin; Ant A, antimycin A."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "A. Representative images of mice and body weight graph following the expression of mitochondrial ATPIF1H49K (days of doxycycline) (wt, n= 12; LowOXPHOS, n= 12)."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "(K, L). 14C(u)-glucose uptake (K) and oxidation to CO2 (L) in myocytes expressing or not the ATP synthase inhibitor ATPIF1H49K. Bars are the mean ± s.e.m. of n= 3 experiments, 6 replicas/condition. Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype *p &lt;0.05 when compared to wt by ANOVA and Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "T. LD formation upon ATP synthase inhibition (ATPIF1H49K) in starved myocytes after 24 hours of palmitate supplementation in the presence or absence of BCAA. Blue: DAPI, nuclei; green: BODIPY-positive LDs. Histograms show the quantification expressed as the number of LDs/nuclei. Bars are the mean ± s.e.m. of n= 3 experiments, 12 fields/condition. U. LD formation upon ATP synthase inhibition (5µM oligomycin) in myocytes after 24 hours of palmitate supplementation. Histograms show the quantification expressed as fold of control of the BODIPY/DAPI fluorescence intensity. 7 fields/condition. Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype *p &lt;0.05 when compared to wt by ANOVA and Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "D, E). oxidation (D) and incorporation into lipids (E) in myocytes expressing or not ATPIF1H49K. Bars are the mean ± s.e.m. of n= 3 experiments, 6 replicas/condition. Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype *p &lt;0.05 when compared to wt by ANOVA or Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "M. Skm relative expression of the GLUT1, GLUT3, PPARβ/δ and PGC1α by qPCR (wt, n= 5; LowOXPHOS, n= 5). Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype *p &lt;0.05 when compared to wt by ANOVA or Student's t-test."
NCBI gene IDs:
"PPARβ/δ: 19015
PGC1α: 19017
GLUT1: 20525
GLUT3: 20527"
Figure legend: "N. qPCR relative expression of myokines (wt, n= 5; LowOXPHOS, n= 5). Red or blue color represents higher or lower % expression, respectively, compared to that in wt. SPARC, osteonectin; OSTN, musclin; LIF, interleukin 6 family cytokine; FKN, fractalkine; FSTL1, follistatin-like protein 1; FNDC5, irisin; ADIPOQ, adiponectin. Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype *p &lt;0.05 when compared to wt by ANOVA or Student's t-test."
NCBI gene IDs:
"adiponectin: 11450
ADIPOQ: 11450
FKN: 13051
fractalkine: 13051
FNDC5: 384061
irisin: 384061
follistatin-like protein 1: 14314
FSTL1: 14314
interleukin 6 family cytokine: 16878
LIF: 16878
musclin: 239790
OSTN: 239790
osteonectin: 20692
SPARC: 20692"
Figure legend: "I. MitoSox staining in myocytes expressing or not ATPIF1H49K. The right histogram shows the quantification of mitochondrial ROS. Bars are the mean ± s.e.m. of n= 3 experiments, 12 replicas/condition. Data information: Bars are the mean ± s.e.m. of the indicated (n) mice/genotype. *p &lt;0.05 when compared to wt by Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "I. MitoSox staining in myocytes expressing or not ATPIF1H49K. The left scheme illustrates where each ETC inhibitor works. The right histogram shows the quantification of mitochondrial ROS. Bars are the mean ± s.e.m. of n= 3 experiments, 12 replicas/condition. Data information: *, # p &lt;0.05 when compared to wt or LowOXPHOS, respectively, by ANOVA and Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "J. MitoSox staining in myocytes expressing or not ATPIF1H49K. The left scheme illustrates where each CII inhibitor works. The right histogram shows the quantification of mitochondrial ROS. Bars are the mean ± s.e.m. of n= 3 experiments, 12 replicas/condition. Data information: *, # p &lt;0.05 when compared to wt or LowOXPHOS, respectively, by ANOVA and Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "(F, G) FFA β-oxidation in C2C12 myocytes transfected with CRL or ATPIF1H49K plasmids (F) and in primary myotubes from wt and LowOXPHOS mice (G), treated (green bars) or not with 2 µM edaravone. Bars are the mean ± s.e.m. of n= 3 experiments, 9 replicas/condition. Data information: *p &lt;0.05 when compared to wt by ANOVA and Student's t-test."
NCBI gene IDs:
"ATPIF1: 93974"
Figure legend: "C. qPCR relative expression of TNFα and PGC1α in myocytes treated for 24 h with 2μM edaravone (green bars), 10nM MitoQ (purple bars) or 1 mM NAC (blue bars). 6 replicas/condition."
NCBI gene IDs:
"PGC1α: 19017
TNFα: 21926"
Figure legend: "A. Survival curves for WT and Spns2-/- CLP  models without antibiotic therapy within 72 hours (n=13 for each group). Data information: Data are presented as percentage (A N represents biological replicates. P values were determined by log-rank test (A *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001; n.s., not significant."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "E. Survival curves for WT (n=12) and Spns2-/- (n=17) CLP  models receiving meropenem  treatment within 7 days. Data information: Data are presented as percentage E) N represents biological replicates. P values were determined by log-rank test E) *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001; n.s., not significant."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "B. Oxygen consumption rates  (OCR) and quantifications of  basal respiration, maximal respiration, ATP  production, and proton leakage in resting WT, Spns2-/-, and 1 μM S1P  pre-treated Spns2-/- PMs  (n=4 for each group). Data information: Data are presented as mean ± s.e.m. (B N represents biological replicates. P values were determined by unpaired t-test. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001; n.s., not significant."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "A. Fragmented mitochondrial morphology  in Spns2-/- PMs  revealed by  ransmission electron microscopy. Zoom-in view of the area in the red frame are shown below. Scale bars: 1 μm. B. The lengths of 38 and 47 mitochondria  from WT and Spns2-/- PMs, respectively (n=1 for each group). Data information: Data are presented as mean ± s.e.m. N represents biological replicates. P values were determined by unpaired t-test. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001; n.s., not significant."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "H. Protein levels of Slc25a12  and Slc25a13  in WT, Spns2-/-, and 1 μM S1P  pre-treated Spns2-/- PMs  (n=3 for each group). Data information: Data are presented as mean ± s.e.m. N represents biological replicates. P values were determined by unpaired t-test. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001; n.s., not significant."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "F. Attenuated activities  of total SOD  and catalase  in oxamate- and S1P-treated Spns2-/- PMs  (n=3 to 4 for each group). Data information: Data are presented as mean ± s.e.m. N represents biological replicates. P values were determined by unpaired t-test. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "A, B. Reduced gene expression  of inflammatory cytokines in Spns2-/- PMs  treated with either oxamate  (A) or mitoquinone  (B) (n=3 per time point  for each group). Data information: Data are presented as mean ± s.e.m. N represents biological replicates. P values were determined by unpaired t-test. **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "C. Activation of Spns2/S1P  signaling and suppression of the lactate-ROS  axis promoted the survival of Spns2-/- sepsis  models induced by heat-killed E. coli through the alleviation of hyerinflammation  (n=5 for Spns2-/- control group; n=4 for each treatment group). Data information: Data are presented as mean ± s.e.m. N represents biological replicates. P values were determined by unpaired t-test. **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "A. S1P  treatment restored inflammatory response in Spns2-/- PMs  after 6 hours post- LPS  challenge (n=3 per time point for each group). Data information: Data are presented as mean ± s.e.m. (A N represents biological replicates. P values were determined by unpaired t-test (A *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "B. Enhancement of Spns2/S1P  signaling significantly improved the survival  of CLP  models without antibiotic therapy (n=8 for Spns2-/- saline  group; n=14 for Spns2+/- group; n=12 for Spns2-/- S1P  treatment group). Data information: Data are presented as percentage (B). N represents biological replicates. P values were determined by log-rank test (B). *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"
Figure legend: "C. Enhancement of Spns2/S1P  signaling restored inflammatory response in Spns2-/- CLP  models at 12 hours post-infection (n=6 for each group). Data information: Data are presented as mean ± s.e.m. C) N represents biological replicates. P values were determined by unpaired t-test C) *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001."
NCBI gene IDs:
"Spns2: 100270678"