EMBO/SourceData huggingface dataset:
Figure legend: "Accumulation of intracellular Aβ1−42 in neurons expressing the AP2-α−WT (WT: 1.14±0.18 , WT=28 and Mut=33 neurons, N=4 biological replicates). Scale bars: 20µm, 2µm (inserts)."
NCBI gene IDs:
"AP2-α: 21418"
Figure legend: "Accumulation of intracellular Aβ1−42 in neurons overexpressing the AP2-α-Mut comparing to neurons expressing the AP2-α−WT (WT: 1.14±0.18, Mut: 2.06±0.29, p=0.011, WT=28 and Mut=33 neurons, N=4 biological replicates). Scale bars: 20µm, 2µm (inserts). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP2-α: 21418"
Figure legend: "Accumulation of intracellular Aβ1-42 is rescued upon BACE1 knock-down (WTScr: 2.76±0.20 WTshBACE1: 2.52±0.12 27 to 39 neurons per condition, N=3 biological replicates). Scale bar: 10µm."
NCBI gene IDs:
"BACE1: 23821"
Figure legend: "Accumulation of intracellular Aβ1-42 is rescued upon BACE1 knock-down in AP-2µ KO neurons (WTScr: 2.76±0.20, KOScr: 3.94±0.21; WTshBACE1: 2.52±0.12; KOshBACE1: 2.74±0.21; pWTScr vs pKOScr=0.000, pKOscr vs pKOshBACE1=000; pWTscr vs pWTshBACE1=0.826; pWTshBACE1 vs pKOshBACE1=0.863; 27 to 39 neurons per condition, N=3 biological replicates). Scale bar: 10µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA in (T) n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773
BACE1: 23821"
Figure legend: "(U, V) Levels of intracellular Aβ1−42 are significantly upregulated in neurons overexpressing the HA-BACE1-LL/AA-GFP comparing to neurons expressing the HA-BACE1-GFP (BACE1: 0.78±0.90, BACE1-LL-AA: 1.99±0.33, p=000, 28-29 neurons per condition, N=3 biological replicates). Scale bar, 50µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"GFP: 
HA: 
BACE1: 23621"
Figure legend: "(A, B) AP-2α levels normalized to GAPDH are significantly decreased in lysates of iPSC-derived neurons from late-onset AD patients carrying the TREM2 p.R47H (AD-TREM2-2 and AD-TREM2-4) variant compared to healthy controls (CON8 and CON9) set to 100% (ADAP-2α: 52.42±8.04%, p=0.020, N=3 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"TREM2: 54209"
Figure legend: "(C) Confocal images of either AP-2 WT (AAV-GFPCamkIIα-labelled) or AP-2 KO (AAV-Cre-GFPCamkIIα-labelled) granule neurons in the dentate gyrus of 13-week-old mice. Scale bars: 20µm, 2µm (inserts)."
NCBI gene IDs:
"GFP: 
AP-2: 11773
CamkIIα: 12322
Cre: 2777477"
Figure legend: "(D) Loss of AP-2µ significantly decreases the number of spines on granule neurons (WT: 12.63±0.88, KO: 8.07±0.45, p=0.004, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(E, F) Overlap coefficient between the presynaptic marker Bassoon and the postsynaptic marker PSD95 is significantly decreased in the entorhinal cortex of AP-2µ KO mice compared to the WT (WT: 0.45±0.02, KO: 0.33±0.06, p=0.034, N=3 biological replicates). Scale bar, 750nm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "Lost preference for the novel object (nov) comparing to the familiar object (fam) in AP-2µ KO mice tested 24h after training in NOR Representative heatmaps are shown in (G)."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "Lost preference for the novel object (nov) comparing to the familiar object (fam) in AP-2µ KO mice tested 24h after training in NOR (WTfam: 27.51±6.77, WTnov: 72.49±6.77, pWTfam vs WTnov=0.000; KOfam: 43.9±13.12, KOnov: 59.91±13.12, pKOfam vs KOnov=0.469, N=7 WT and 8 KO mice). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(I, J) AP-2µ KO mice are significantly more active in the open field compared to the WT (WT: 1663±139.08%, KO: 3976±802.30%, p=0.020, N=7 WT and 8 KO mice). Representative heatmaps are shown in (I). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(K, L) Overlap coefficient between Bassoon and PSD95 is rescued upon BACE1 KD in AP-2µ KO neurons (WTscr: 0.39±0.02; KOscr: 0.24±0.01; WTshBACE1: 0.37±0.02; KOshBACE1: 0.33±0.02; pWTscr vs pKOscr&lt;0.000; pKOscr vs pKOshBACE1=0.010; pWTscr vs pWTshBACE1=0.813; pWTshBACE1 vs pKOshBACE1=0.623, 22-24 neurons for each condition, N=3). Scale bars: 10µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773
BACE1: 23821"
Figure legend: "Neuronal loss is rescued in the entorhinal cortex of AP-2µ mice haploinsufficient for BACE1 (AP-2WT/BACEWT: 2.25±0.08 N=9 sections from N=3 for each genotype). Scale bar: 100 μm."
NCBI gene IDs:
"AP-2: 11773
AP-2µ: 11773
BACE: 23821
BACE1: 23821"
Figure legend: "Neuronal loss is rescued in the entorhinal cortex of AP-2µ KO mice haploinsufficient for BACE1 AP-2KO/BACEWT: 1.51±0.08, AP-2WT/BACEHET: 1.80±0.07, AP-2KO/BACEHET: 2.00±0.07; pAP-2WT/BACE1WT vs pAP-2KO/BACE1WT&lt;0.000; pAP-2WT/BACE1WT vs pAP-2WT/BACE1HET=0.001; pAP-2KO/BACE1WT vs pAP-2KO/BACE1HET=0.000; p AP-2WT/BACE1HET vs AP-2KO/BACE1HET=0.268, N=9 sections from N=3 for each genotype). Scale bar: 100 μm. Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2: 11773
AP-2µ: 11773
BACE: 23821
BACE1: 23821"
Figure legend: "(P, Q) APP-CTF levels are rescued in the entorhinal cortex of AP-2µ KO mice haploinsufficient for BACE1 (AP-2WT/BACEWT:427574±24021, AP-2KO/BACEWT: 538895±34662, AP-2WT/BACEHET: 389870±19842, AP-2KO/BACEHET: 395389±21853; pAP-2WT/BACE1WT vs pAP-2KO/BACE1WT=0.017; pAP-2KO/BACE1WT vs pAP-2KO/BACE1HET=0.001; p AP-2WT/BACE1HET vs AP-2KO/BACE1HET=0.979; 13-15 sections, N=3 mice for each genotype). Scale bars: 40µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA n.s.-non-significant.* indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2: 11773
AP-2µ: 11773
BACE: 23821
BACE1: 23821"
Figure legend: "(A, B) Flies form well-organized compound eyes when they carry GMR-GAL4 without a target gene (A) but develop rough eyes when GMR-GAL4 drives expression of UAS-Foxn1 (B). Panel B shows the typical severity of the rough-eye phenotype. Data information: Representative phenotypes are shown in all panels. Scale bars, 100 μm."
NCBI gene IDs:
"Foxn1: 15218
GAL4: 855828"
Figure legend: "(C, D) Bristles are pigmented and properly shaped when flies carry hs-GAL4 without a target gene (C) but are often white, bent, and shortened when hs-GAL4 drives expression of UAS-Foxn1 (D). Flies in panels C and D were raised at 25°C. Arrows denote examples of white bristles. Data information: Representative phenotypes are shown in all panels. Scale bars, 100 μm."
NCBI gene IDs:
"Foxn1: 15218
GAL4: 855828"
Figure legend: "Representative skin phenotypes of Foxn1nu/nu (Nude), or KRT14-cre; Aff4flox/flox (Aff4 cko) mice are shown macroscopically or histologically after hematoxylin and eosin staining (H-N). The phenotypes compared are as follows: hair coats, 4-month-old adults; (E-G) vibrissae, P7; (H) hair follicles with growing hairs, juveniles; (I-K) regressing hair follicles, juveniles; (L-N) epidermis, P9. In panel H, micrographs from left to right are WT, Nude, and Aff4 cko. In panels I-K, HF marks examples of hair follicles. In panels L-M, arrowheads indicate the dermal/epidermal border; arrows mark examples of bent hair shafts. Scale bars: H, L-N, 20 µm; I-K, 40 µm."
NCBI gene IDs:
"Aff4: 93736
cre: 2777477
Foxn1: 15218
KRT14: 16664"
Figure legend: "(A-C) Thymi from E18.5 embryos are shown: (A) intact with genotypes indicated, or (B, C) after sectioning, immunofluorescent staining for DLL4 (red), and counterstaining of DNA (blue). Sectioned samples are wild-type (B) or Aff4ko/ko; Foxn1+/- (C). Scale bars: A, 1 mm; B-C, 50 µm."
NCBI gene IDs:
"Aff4: 93736
Foxn1: 15218"
Figure legend: "(C-E) KRT86 (red) is shown by immunofluorescence in skin sections from wild-type (WT), Foxn1 null, or Aff4 cko mice at P9. DNA is stained blue. HS marks examples of hair shafts. Scale bar, 40 µm."
NCBI gene IDs:
"Aff4: 93736
Foxn1: 15218"
Figure legend: "Graphs present pre-mRNA (B, C) assays of Krt86 using skin from mutant mice (Foxn1 null, Aff4 cko) or corresponding wild-type controls at P6 (B, C) All values are derived from 3 independent experiments and presented as mean ± S.D. Pre-mRNA measurements were analyzed statistically using two-way ANOVA with post-hoc Sidak's multiple comparison test at a 95% confidence interval."
NCBI gene IDs:
"Aff4: 93736
Foxn1: 15218
Krt86: 16679"
Figure legend: "Graphs present ChIP (D-G) assays of Krt86 using skin from mutant mice (Foxn1 null, Aff4 cko) or corresponding wild-type controls at P7 (D-G). ChIP assays were performed with antibodies to FOXN1 or AFF4 as indicated. All values are derived from 3 independent experiments and presented as mean ± S.D. ChIP assays were analyzed statistically using one-way ANOVA with post-hoc Tukey's multiple comparison test at a 95% confidence interval. ****, p ≤ 0.0001."
NCBI gene IDs:
"Aff4: 93736
Foxn1: 15218
Krt86: 16679"
Figure legend: "(B-G) Graphs present ChIP assays of Krt86 using antibodies to total (B, C), P-Ser5 (D, E), or P-Ser2 (F, G) Pol II and skin from mutant mice (Foxn1 null, Aff4 cko) or corresponding wild-type controls at P7. All values are derived from at least 3 independent experiments and presented as mean ± S.D. Assay results were analyzed statistically using one-way ANOVA with post-hoc Tukey's multiple comparison test at a 95% confidence interval. ****, p ≤ 0.0001."
NCBI gene IDs:
"Aff4: 93736
Foxn1: 15218
Krt86: 16679"
Figure legend: "A. qRT-PCR analysis of RNA samples from normal human skin (NS) and AK for activin βA (INHBA), normalized to hypoxanthine phosphoribosyltransferase (HPRT). Expression levels in one of the normal skin samples were set to 1. Scatter plots are shown. NS: N=4; AK: N=21. ns = non-significant (Mann-Whitney test)."
NCBI gene IDs:
"HPRT: 
INHBA: 3624"
Figure legend: "B. qRT-PCR analysis of RNA samples from normal back skin of wild-type mice (N=4, n=4), non-tumorigenic back skin from HPV8/wt mice (N=7, n=9) and papillomas from HPV8/wt transgenic mice (N=8, n=11) for Inhba, normalized to glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Expression levels in one of the wild-type back skin samples were set to 1. Statistical significance was determined using 1-way ANOVA and Bonferroni`s Multiple Comparisons Test of log-transformed data. N, number of mice; n, number of biopsies."
NCBI gene IDs:
"Gapdh: 
Inhba: 16323"
Figure legend: "F. Kinetics of tumor incidence in HPV8/wt and HPV8/Act mice; N=20 mice per genotype. Statistical significance was determined using Log-rank (Mantel-Cox) test."
NCBI gene IDs:
"Act: 16323"
Figure legend: "A,B. Representative pictures of ear sections from 10 week-old mice stained for δTcR (green); counterstained with Hoechst (blue) (A) and quantification of δTcR+ cells in the ear epidermis (B). Dotted lines indicate the epidermal-dermal border. Scale bar: 20μm. N=6 wt/wt mice, N=7 HPV8/wt mice, N=6 wt/Act mice and (N=8) HPV8/Act mice."
NCBI gene IDs:
"Act: 16323"
Figure legend: "C. Quantification of βTcR+ cells in ear skin cryosections from 10 week-old wt/wt (N=5); HPV8/wt (N=5); wt/Act (N=8) or HPV8/Act (N=8) mice."
NCBI gene IDs:
"Act: 16323"
Figure legend: "G. Representative pictures of ear cryosections from 10 week-old mice stained with antibodies against Foxp3 (red) and counterstained with Hoechst (blue). Insert shows higher magnification of the Foxp3-positive cells in wt/Act skin. Dotted lines indicate the epidermal-dermal border. Scale bar: 50μm."
NCBI gene IDs:
"Act: 16323"
Figure legend: "H. Kinetics of tumor incidence in HPV8/Act/wt (N=29), HPV8/Act/CD4KO (N=10), HPV8/wt/wt (N=23) and HPV8/wt/CD4KO (N=22) mice per genotype. Statistical significance was determined using the Log-rank (Mantel-Cox) test; ns = non-significant."
NCBI gene IDs:
"CD4: 12504
Act: 16323"
Figure legend: "A,B. Representative pictures of ear skin cryosections from 10-12 week-old mice stained with anti-CD206 antibodies (green); counterstained with Hoechst (blue) (A), and quantification of CD206+ area as a percentage of total dermal area (B) in wt/wt (N=3), HPV8/wt (N=7), wt/Act (N=2) or HPV8/Act (N=8) mice. The basement membrane is indicated with a white dotted line."
NCBI gene IDs:
"Act: 16323"
Figure legend: "D. Quantification of CD206+ area as a percentage of total dermal area in wt (N=7) and Act (N=6) mice in back skin treated 8x with TPA (Antsiferova et al, 2011)."
NCBI gene IDs:
"Act: 16323"
Figure legend: "E. Abundance of MerTK+CD64+ macrophages analyzed by flow cytometry of pre-tumorigenic ear skin from 12 week-old mice expressing the CCR2-CFP-DTR transgene (wt/CCR2) or double-transgenic animals (Act/CCR2), untreated or treated with diphtheria toxin (+DT) 3 times every 48h and analyzed 24h after the last injection. Percentage of CD45+CD11b+MerTK+CD64+ macrophages among live cells is shown. N=3 control wt/CCR2 and Act/CCR2 mice; N=4 DT-injected wt/CCR2 and Act/CCR2 mice. Representative flow cytometry scatter plots are shown in Appendix Fig S1E."
NCBI gene IDs:
"CCR2: 12772
Act: 16323"
Figure legend: "C. Differential expression of genes was analyzed using the edgeR software package. IPA was performed with filtered lists of up- and downregulated genes that exhibited an FDR-value of &lt;0.1 in different genotype comparisons (wt/Act vs wt/wt; HPV8/Act vs HPV8/wt; HPV8/Act vs wt/wt). The number of differentially expressed genes used for analysis is indicated. Data are presented as pseudo-heatmap with magnitudes of Activation Z-scores color-coded as indicated in the legend; all non-grey comparisons are -log10(p-value)&gt;2.5 unless otherwise indicated."
NCBI gene IDs:
"Act: 16323"
Figure legend: "D. Gene Set Enrichment Analysis (GSEA) was performed to compare transcriptomes of genotype comparisons (wt/Act vs wt/wt; HPV8/Act vs HPV8/wt; HPV8/Act vs wt/wt, [all]/Act vs [all]/wt) to 3 published datasets of Tumor-Associated Macrophages (TAM) (Franklin et al. 2014; Ojalvo et al, 2009; Galletti et al, 2016). Gene sets are composed of top genes upregulated (TAM UP) or downregulated (TAM DOWN) in TAM vs normal tissue macrophages. Positive Normalized Enrichment Score (NES) corresponds to enrichment of gene set in activin-upregulated genes; negative NES corresponds to enrichment in activin-downregulated genes. Data are presented as pseudo-heatmap with magnitudes of NES color-coded as indicated in the legend; all non-grey comparisons are FDR&lt;0.10."
NCBI gene IDs:
"Act: 16323"
Figure legend: "E. Differential GSEA was performed on combined genotype comparison [all]/Act vs [all]/wt and two datasets of human AK vs normal skin (GSE2503; Nindl et al, 2006) and GSE63107 to compare these transcriptomes to gene sets derived from top upregulated genes in these datasets (top); and gene sets derived from top upregulated genes in datasets of differential human macrophage activation (IFN-γ, TNF-α, LPS, IL-4 vs non-treated) and selected human myeloid/lymphoid cell type-specific gene expression profiles (macrophage vs monocyte, dendritic cell, and T-cell) (Xue et al, 2014; bottom). Activin target genes enriched in the AK transcriptomes are shown (right). Data are presented as pseudo-heatmaps with magnitudes of Normalized Enrichment Scores (NES) and Log2(Fold Change) color-coded as indicated in the respective legends; all non-grey comparisons are FDR&lt;0.05 for NES and p&lt;0.10 for Fold Change."
NCBI gene IDs:
"Act: 16323"
Figure legend: "G. Heatmap of 28 differentially expressed genes (FDR≤0.05, |log2Ratio|≥1) overlapping in all 3 comparisons (wt/Act vs wt/wt mice, HPV8/Act vs HPV8/wt and HPV8/Act vs wt/wt). Genes with a published pro-tumorigenic function are highlighted in red."
NCBI gene IDs:
"Act: 16323"
Figure legend: "A,B,F. Expression of Cx3cr1 (A), Spp1 (B) relative to Rps29 was analyzed by qRT-PCR in peritoneal macrophages, resident or inflammatory, treated in vitro with 10 ng/ml of activin A (Act) or untreated (Ctrl, arbitrarily set to 1). Results are combined from 2-5 independent experiments performed with cells pooled from at least 5 wt mice."
NCBI gene IDs:
"Rps29: 
Cx3cr1: 13051
Spp1: 20750"
Figure legend: "E. Ear skin sections from 10 week-old mice stained with anti-CD68 (red) or anti-osteopontin antibody (green); counterstained with Hoechst (blue). Osteopontin-positive macrophages in wt/Act and HPV8/Act mice are indicated with arrows. Representative of N=2 wt/wt and N=3 HPV8/wt, wt/Act and HPV8/Act mice. Boxed areas in top panel are shown at higher magnification in bottom panel. Scale bar top: 100μm, bottom: 20μm."
NCBI gene IDs:
"Act: 16323"
Figure legend: "A,B,F. Expression of Arg1 (F) relative to Rps29 was analyzed by qRT-PCR in peritoneal macrophages, resident or inflammatory, treated in vitro with 10 ng/ml of activin A (Act) or untreated (Ctrl, arbitrarily set to 1). Results are combined from 2-5 independent experiments performed with cells pooled from at least 5 wt mice."
NCBI gene IDs:
"Rps29: 
Arg1: 11846"
Figure legend: "H,I. Gelatin zymography of ear skin lysates from 10 week-old mice. Inverted image of a representative gel with 2 (wt/wt) or 3 (HPV8/wt, wt/Act, HPV8/Act) mice per genotype is shown in (H), quantification of the results obtained in 2 independent experiments is shown in (I). N=5 wt/wt, N=6 HPV8/wt, wt/Act and HPV8/Act mice."
NCBI gene IDs:
"Act: 16323"
Figure legend: "A. Kinetics of tumor incidence in HPV8/Act mice treated for 5 weeks with 50mg/kg anti-CSF1R antibody (AFS98) or with control rat IgG. N=10 mice per treatment group."
NCBI gene IDs:
"Act: 16323"
Figure legend: "F. Expression of Mmp12 relative to Rps29 was analyzed by qRT-PCR in total ear skin from mice treated with anti-CSF1R antibody or with rat IgG. Expression in one of the IgG-injected mice was set to 1. N=10 per group."
NCBI gene IDs:
"Rps29: 
Mmp12: 17381"
Figure legend: "(A) Representative micrographs  of human skin  sections stained with hematoxylin-eosin (H&amp;E) (left) and anti-IL-38 antibody (right) from normal patients  (n = 11) and tumors of cSCC patients  (n = 13). Scale bars represent 100 μm. The graph shows the quantification of mean  IL-38 expression   per high-powered field in tissues. (B) Relative expression   of  IL-38 in human  normal tissues (n = 9) and cSCC  (n = 18) were analyzed using Geo Datasets (GSE98767). Error bars represent the mean ± SD. All data are biological replicates. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; p values were calculated using Student's t test."
NCBI gene IDs:
"IL-38: 84639"
Figure legend: "(C and D) The  dorsal hair of normal C57/BL6 mice  were shaved and treated with DMBA/TPA  twice a week for 32 weeks to induce skin  tumors. (C) Representative micrographs   of mouse normal skin  (n = 6) and tumor (n = 6) sections stained  with anti-IL-38  antibody. The graph shows the quantification of mean  IL-38 expression   per high-powered field in tissues. Scale bars represent 100 μm. (D) Relative expression   levels of Il-38 in normal skin  (n = 5) and tumors (n = 5) of mice  were quantified using qPCR. Error bars represent the mean ± SD. All data are biological replicates. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; p values were calculated using Student's t test."
NCBI gene IDs:
"Il-38: 215274"
Figure legend: "(B) Representative photographs  of Il-38f/f (n = 12) and K14Cre/+-Il-38f/f mice  (n = 8) treated with DMBA/TPA for 32 weeks. White triangles indicate the tumors  on the back of mice. (C Average tumor number (C) per mouse  from Il-38f/f (n = 12) and K14Cre/+-Il-38f/f mice  (n = 8) treated with DMBA/TPA.. Error bars represent the mean ± SD (C) All data are biological replicates. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; p values were calculated using Student's t test (C"
NCBI gene IDs:
"Cre: 2777477
Il-38: 215274
K14: 3861"
Figure legend: "(A) Representative immunohistochemical staining micrographs  of γH2AX  in the skin  of Il-38f/f (n = 5) and   K14Cre/+-Il-38f/f (n = 5) mice  after treated with DMBA  for 24h. Red triangles indicate the γH2AX+ positive cells. Scale bars represent 100 μm. The graph shows the number of  γH2AX+ cells per high-powered field. Data information: Error bars represent the mean ± SD. All data are biological replicates. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; p values were calculated using Student's t test."
NCBI gene IDs:
"Cre: 2777477
Il-38: 215274
K14: 3861"
Figure legend: "(A The  dorsal hair  of normal C57/BL6 mice  were shaved and treated with DMBA/TPA  twice a week for 3 weeks to induce the skin inflammation. (A) Representative micrograph  sections stained  with hematoxylin-eosin  (H&amp;E) from the skin of Il-38f/f (n = 5) and  K14Cre/+-Il-38f/f mice (n = 5) Scale bars represent 100 μm. The graph shows average epidermal  thickness."
NCBI gene IDs:
"Cre: 2777477
Il-38: 215274
K14: 3861"
Figure legend: "B) The dorsal hair  of normal C57/BL6 mice  were shaved and treated with DMBA/TPA  twice a week for 3 weeks to induce the skin inflammation. (B) Representative micrograph  sections stained  with anti-Ki67  antibody from the skin  of Il-38f/f (n = 6) and K14Cre/+-Il-38f/f mice  (n = 6). Scale bars represent 100 μm. The graph shows average numbers of Ki67+ cells per high-powered field."
NCBI gene IDs:
"Cre: 2777477
Il-38: 215274
K14: 3861"
Figure legend: "(G and H) Representative images of the  scratch assay  (left) and  wound closure rate (right) of A431  cells (n = 5) after overexpression (G) or knockdown (H) of IL-38. Error bars represent the mean ± SD. All data are biological replicates. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; p values were calculated using Student's t test. NC, Negative control; LV, Lentivirus; Oe, Overexpression."
NCBI gene IDs:
"IL-38: 84639"
Figure legend: "(I and J) Representative  western blot  bands indicating cyclin E and cyclin A  in A431  cells after  IL-38 overexpression (I) or knockdown (J). NC, Negative control; LV, Lentivirus; Oe, Overexpression."
NCBI gene IDs:
"IL-38: 84639"
Figure legend: "(C) Cell extracts prepared from  293T  cells transfected with a mock mammalian expression vector (left) or an expression vector encoding  IL-1Rrp2 (right) were probed using anti-phospho-JNK, anti-JNK, or anti-β-actin antibodies after stimulation with IL-38 (200 ng/ml) or  IL-36β (200 ng/ml) at different times."
NCBI gene IDs:
"IL-1Rrp2: 8808"
Figure legend: "(D) Cell extracts prepared from 293T  cells transfected with an expression vector encoding IL-1Rrp2 were probed using anti-phospho-JNK, anti-JNK, or anti-β-actin antibodies after stimulation with IL-38 at different concentrations."
NCBI gene IDs:
"IL-1Rrp2: 8808"
Figure legend: "(F) Cell extracts prepared from 293T  cells transfected with HA-tagged full-length or deletion mutants of IL-1Rrp2, were probed using anti-HA  or anti-β-actin antibodies. Oe, overexpression."
NCBI gene IDs:
"HA: 
IL-1Rrp2: 8808"