EMBO/SourceData huggingface dataset:
Figure legend: "MITOL KO facilitated IRE1α oligomerization. MEFs were transfected with IRE1α-GFP expression vector for 24 hours prior to Tu treatment for indicated periods (A). The right panels show 5-fold magnification images of the boxed regions. Percentages of cells with IRE1α-foci were calculated from 100 cells by visual inspection in each independent experiment (A). To evaluate the oligomerization level of endogenous IRE1α, cells without any transfection were solubilized and separated by sucrose density-gradient centrifugation, followed by immunoblotting with anti-IRE1α antibody (B)."
NCBI gene IDs:
"GFP: 
IRE1α: 2081
MITOL: 69104"
Figure legend: "IRE1α oligomers were stabilized by MITOL KO. MEFs transfected with IRE1α-GFP were treated with Tu. After 4 hours, these cells were washed with PBS and subsequently re-fed with fresh media for indicated periods. The right panels show 5-fold magnification images of the boxed regions. Percentages of cells with IRE1α-foci were calculated from 100 cells by visual inspection at indicated periods after wash out of Tu in each independent experiment. Scale bar represents 10 µm."
NCBI gene IDs:
"GFP: 
IRE1α: 2081
MITOL: 69104"
Figure legend: "Sustained IRE1α autophosphorylation after wash out of Tu in MITOL-KO MEFs. MEFs were treated with Tu for 4 hours and washed with PBS and re-fed with fresh media for indicated periods, following by immunoblotting anti-IRE1α antibody after Phos-tag SDS-PAGE. Error bars represent SD (n=3)."
NCBI gene IDs:
"MITOL: 69104"
Figure legend: "MITOL deletion promoted the interaction between IRE1α and BIM under ER stress. MITOL-depleted HEK293 cells (KO) were generated using CRISPR-Cas9 system. Cells were co-transfected with indicated vectors 24 hours prior to Tu treatment for 12 hours. Cells were immunoprecipitated with anti-FLAG antibody, followed by immunoblotting with indicated antibodies."
NCBI gene IDs:
"CRISPR: 
MITOL: 54708"
Figure legend: "Overexpression of IRE1α K481R induced apoptosis. MEFs were transfected with indicated vectors. After 24 hours, these cells were stained with Annexin V-FITC"
NCBI gene IDs:
"IRE1α: 2081"
Figure legend: "Overexpression of IRE1α K481R induced apoptosis. or subjected by immunoblotting with indicated antibodies (D)."
NCBI gene IDs:
"IRE1α: 2081"
Figure legend: "Enhanced RIDD activity in IRE1α K481R. MEFs were transfected with indicated vectors 24 hours prior to analysis (E, F). The RIDD activity was measured by qRT-PCR."
NCBI gene IDs:
"IRE1α: 2081"
Figure legend: "Hyper-oligomerization of IRE1α K481R. MEFs were transfected with IRE1α-GFP or IRE1α K481R-GFP. After 24 hours, these cells were exposed with Tu for indicated periods and GFP signals were observed. The right panels show 5.5-fold magnification images of the boxed regions. Percentages of cells with IRE1α-foci were calculated from 100 cells by visual inspection in each independent experiment."
NCBI gene IDs:
"GFP: 
IRE1α: 2081"
Figure legend: "IRE1α regulation by MITOL occurred in the MAM-rich mitochondrial fraction. Crude mitochondrial fraction containing MAM (Mito plus MAM) and the cytosolic fraction containing ER (Cyto plus ER) were isolated from HEK293 cells transfected with indicated vector and subsequently immunoprecipitation assay was performed to detect the level of IRE1α ubiquitylation (D)."
NCBI gene IDs:
"MITOL: 54708"
Figure legend: "Knockdown of PACS2 impaired the interaction of MITOL with IRE1α. HEK293 cells were transfected with either scramble RNA or siPACS2 and indicated vectors for 24 hours prior to immunoprecipitation assay."
NCBI gene IDs:
"PACS2: 23241"
Figure legend: "Knockdown of PACS2 inhibited IRE1α ubiquitylation by MITOL. HEK293 cells were transfected with either scramble RNA, siPACS2 and indicated vectors 24 hours prior to immunoprecipitation assay."
NCBI gene IDs:
"PACS2: 23241"
Figure legend: "Knockdown of Mfn2 inhibited IRE1α ubiquitylation by MITOL. HEK293 cells were transfected with either scramble RNA, siMfn2 and indicated vectors 24 hours prior to immunoprecipitation assay."
NCBI gene IDs:
"Mfn2: 9927"
Figure legend: "MITOL ubiquitylated IRE1α in the spinal cord of mice. Lysates of the spinal cord in mice were immunoprecipitated with anti-IRE1α antibody, followed by immunoblotting with indicated antibodies. WT: MITOLF/F mice, MITOLnestin: MITOLF/F, Nestin-Cre mice."
NCBI gene IDs:
"Cre: 2777477
MITOL: 69104"
Figure legend: "Loss of MITOL led to hyper-oligomerization of IRE1α in the spinal cord under ER stress. 3 month-old WT or MITOLnestin mice were treated with 1 mg/kg Tu for 24 hours. Spinal cord was solubilized with NP-40 lysis buffer and separated by sucrose-density gradient centrifugation."
NCBI gene IDs:
"MITOL: 69104"
Figure legend: "MITOL deletion resulted in hyper-activation of IRE1α RNase in ER stress. WT or MITOLnestin mice were injected with Tu for 24 hours and spinal cord was analyzed by qRT-PCR."
NCBI gene IDs:
"MITOL: 69104"
Figure legend: "Increased ER stress-induced apoptosis in the MITOL-KO spinal cord. 3 month-old WT or MITOLnestin mice were treated with Tu for 24 hours and each spinal cord was stained using TUNEL and hoechst (F) Arrowheads indicate the representative neurons."
NCBI gene IDs:
"MITOL: 69104"
Figure legend: "Increased ER stress-induced apoptosis in the MITOL-KO spinal cord. 3 month-old WT or MITOLnestin mice were treated with Tu for 24 hours and each spinal cord was stained using cresyl violet (G). Arrowheads indicate the representative neurons."
NCBI gene IDs:
"MITOL: 69104"
Figure legend: "(A) Cells in early G1 stably expressing nAC-mCherry and doxycycline-inducible ACTN4-SNAP were analyzed by time-lapse microscopy. ACTN4-SNAP was labeled by SNAP-Cell 647SiR dye (green). Different arrows mark dynamic ACTN4 clusters. The white square 1 is shown as a timeseries on the right. The white square 2 is shown as a magnification below. The white square 3 shows an actin filament decorated with ACTN4 that was analyzed by linescan in Fig 2B. Scale bar overview (nAC) represents 10 µm. Scale bar time series and magnification 5 µm."
NCBI gene IDs:
"SNAP: 
ACTN4: 81"
Figure legend: " Cells in early G1 stably expressing nAC-mCherry and doxycycline-inducible ACTN4-SNAP were analyzed by time-lapse microscopy. ACTN4-SNAP was labeled by SNAP-Cell 647SiR dye (green). The white square 3 shows an actin filament decorated with ACTN4 that was analyzed by linescan in Fig 2B. B) Linescan of an actin filament with associated ACTN4 from square 3 in A. "
NCBI gene IDs:
"SNAP: 
ACTN4: 81"
Figure legend: "(B) NIH3T3 cells stably expressing H2B-mCherry were transfected with siCtrl or siACTN1 + siACTN4. After mitotic exit z-stacks (30 planes) were acquired every 5 min. Nuclei were 3D-reconstructed and volume was measured by Imaris. Data are mean ± SEM from 3 biological replicates with 22-27 (siCtrl) and 32-42 (siACTN1+4) analyzed nuclei; ***p &lt; 0.001 by Wilcoxon test and ****p &lt; 0.0001 by t-test at 90 min."
NCBI gene IDs:
"ACTN1: 109711
ACTN4: 60595
H2B: 68024"
Figure legend: "(C) RPE-1 cells were analyzed as in B. Data are mean ± SEM from 5 biological replicates with 32-37 (siCtrl) and 31-39 (siACTN1+4) analyzed nuclei; ***p &lt; 0.001 by Wilcoxon test and ****p &lt; 0.0001 by Mann Whitney test at 90 min."
NCBI gene IDs:
"ACTN1: 87"
Figure legend: "(E) NIH3T3 cells stably expressing H2B-mCherry and siRNA-resistant ACTN4-NES were transfected with the indicated siRNAs and analyzed as described in B. Data are mean ± SEM from 5 biological replicates with 63-71 (siCtrl), 37-41 (siACTN1+4), 52-58 (siACTN1) and 52-55 (siACTN4) analyzed nuclei; ****p(siACTN4) &lt; 0.0001; ****p(siACTN1+4) &lt; 0.0001 by One Way ANOVA at 90 min."
NCBI gene IDs:
"ACTN1: 109711
ACTN4: 81
ACTN4: 60595
H2B: 68024"
Figure legend: "(F) NIH3T3 cells stably expressing H2B-mCherry and the indicated ACTN4 mutants were transfected with the indicated siRNAs and analyzed as in B. Data are mean ± SEM from 3 biological replicates with 63-69 (ACTN4 wt + siACTN4), 63-71 (ACTN4-NES + siCtrl) and 54-58 (ACTN4-NES + siACTN4) analyzed nuclei; ****p(ACTN4-NES + siACTN4) &lt; 0.0001 by One Way ANOVA at 90 min."
NCBI gene IDs:
"ACTN4: 60595
ACTN4: 81
H2B: 68024"
Figure legend: "(G) NIH3T3 cells expressing siRNA-resistant ACTN4-NES were transfected with siCtrl or siACTN4; chromatin density was calculated from H2B-mCherry fluorescence intensities by total nuclear volumes (intensity/μm3) and normalized to t=0 (mitotic exit); Data are mean ± SEM from 5 biological replicates with 44-48 (siCtrl) and 44-47 (siACTN4) analyzed nuclei; ***p &lt; 0.001 by Wilcoxon test and *p &lt; 0.05 by t-test at 90 min."
NCBI gene IDs:
"ACTN4: 81
H2B: 68024"
Figure legend: "(B) Upper panel shows expression of ACTN4 constructs (green, confocal image) and phalloidin-AF647 staining in the same cells (orange, dSTORM image) scale bar 10 µm; lower panel represents zoom of the indicated areas (white boxes); scale bars 5 µm."
NCBI gene IDs:
"ACTN4: 60595"
Figure legend: "(C) Phalloidin-AF647 localizations per post-mitotic nuclei; data shown as mean ± 2.5-97.5 percentile: 52 ACTN4-wt nuclei, 55 ACTN4ΔCH1-NLS nuclei and 40 nuclei of non-dividing cells (ACTN4-wt) from 5 biological replicates. ***p(ACTN4ΔCH1-NLS post-mitotic) &lt; 0.001, ****p(ACTN4-wt non-dividing) &lt; 0.0001, *p (ACTN4ΔCH1-NLS post-mitotic vs. ACTN4-wt non-dividing) &lt; 0.05 by One Way ANOVA."
NCBI gene IDs:
"ACTN4: 60595"
Figure legend: "(D) Number of filaments in the post mitotic nuclei; as in (C); data shown as mean ± 2.5-97.5 percentile: 52 ACTN4-wt nuclei, 55 ACTN4ΔCH1-NLS nuclei from 5 biological replicates, ***p &lt; 0.001 by t-test."
NCBI gene IDs:
"ACTN4: 60595"
Figure legend: " (E) Actin filament widths for ACTN4-wt and ACTN4ΔCH1-NLS in early G1 nuclei; ); data shown as mean ± 2.5-97.5 percentile with 96 filaments (ACTN4-wt) and 36 filaments (ACTN4ΔCH1-NLS) from 5 biological replicates, **p &lt; 0.01 by t-test. "
NCBI gene IDs:
"ACTN4: 60595"
Figure legend: "RT-qPCR analysis of METTL3 and METTL14 expression (A) in shRNA knockdown METTL3 cells."
NCBI gene IDs:
"METTL14: 57721
METTL3: 56339"
Figure legend: "I HA-METTL14 Co-IP with FLAG-tagged METTL3 domain-deletion mutants. β-actin was used as the loading control."
NCBI gene IDs:
"FLAG: 
METTL3: 56339"
Figure legend: "L Immunoblots showing CHX assay in HEK293T cells. Negative control (NC; no transfected cells) and STUB1-FLAG plasmid-transfected HEK293T cells were exposed to 100 μg/ml CHX for 0, 4, 8, or 12 hours. β-actin was used as the loading control. The METTL14/β-actin densitometric ratio was recorded by ImageJ."
NCBI gene IDs:
"FLAG: 
STUB1: 10273"
Figure legend: "M, N Dot blot assays showing that enforced expression of wild-type STUB1, but not the STUB1H260Q mutant, significantly decreases total m6A levels."
NCBI gene IDs:
"STUB1: 10273"
Figure legend: "B Immunoblots showing the abundance of METTL14 truncated mutants with or without 10 µM MG132 treatment. Histone H4 was used as the loading control. The HA-METTL14/β-actin densitometric ratio was recorded by ImageJ."
NCBI gene IDs:
"METTL14: 57721"
Figure legend: "F Immunoblots showing the levels of METTL14 protein after transfection with METTL14-3KR mutant plasmids with or without 10 µM MG132. β-actin was used as the loading control. The HA-METTL14/β-actin densitometric ratio was recorded by ImageJ. G Immunoblots showing the protein level of METTL14 after transfection with METTL14-3KR mutant plasmids under STUB1 knockdown. β-actin was used as the loading control. The HA-METTL14/β-actin densitometric ratio was recorded by ImageJ."
NCBI gene IDs:
"METTL14: 57721
STUB1: 10273"
Figure legend: "B METTL14 ubiquitination, as detected by IP of METTL3 truncated mutants with anti-HA antibody. The accumulation of Ub and METTL14 was confirmed in whole-cell lysates. β-actin was used as the loading control."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "C Immunoblots showing METTL14 protein levels, as detected by IP in response to METTL3 truncated mutants and STUB1-FLAG. β-actin was used as the loading control."
NCBI gene IDs:
"FLAG: 
METTL3: 56339
STUB1: 10273"
Figure legend: "E-G Following the subcutaneous inoculation of SK-Cha-1-NC (left) and SK-Cha-1-STUB1-FLAG (right) cells into the flanks of male NOD-SCID mice (E), overexpression of STUB1-FLAG inhibited malignant cell proliferation (F) and reduced subsequent tumor size and growth (G) in vivo. Data are means ± SEM. Statistical significance was analyzed by two-way ANOVA (F) or two-tailed unpaired t-test (G) (Five mice, *p &lt; 0.05; **p &lt; 0.01). H-J Following the subcutaneous inoculation of SK-Cha-1-sh-NC (left) and SK-Cha-1-sh-STUB1-1 (right) cells into the flanks of male nude mice (H), STUB1 knockdown promoted malignant cell proliferation (I) and increased subsequent tumor size and growth (J). Data are means ± SEM. Statistical significance was analyzed by two-way ANOVA (I) or two-tailed unpaired t-test (J). (Six mice, *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001)."
NCBI gene IDs:
"FLAG: 
STUB1: 10273"
Figure legend: "Immunoblots (K) showing METTL14 protein levels STUB1-FLAG overexpression in five pairs of mouse tumors. β-actin was used as the loading control. The METTL14 or STUB1-FLAG /β-actin densitometric ratio was recorded by ImageJ."
NCBI gene IDs:
"FLAG: 
STUB1: 10273"
Figure legend: "(b) RNAscope analysis of Lgr5 mRNA in DSS colitis demonstrating loss of Lgr5 transcript (N=3 per condition). Data information: Nuclei are counterstained with Dapi (blue); white dashed lines indicate basement membrane. Scale bars =50µm"
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "(d-e) Compared to untreated controls, DSS treatment ablates Lgr5-GFP+ stem cells and results in functional loss of tdTomato+ lineage tracing as early as d5. Lgr5-GFP+ stem cells reappear by d12 (N=3-4 per condition). Data information: Nuclei are counterstained with Dapi (blue); white dashed lines indicate basement membrane. Scale bars =50µm Data are represented as mean ± SD (e) analyzed using Student's t-test. *P≤0.05, ** P≤0.01."
NCBI gene IDs:
"GFP: 
tdTomato: 
Lgr5: 14160"
Figure legend: "(f) Effects of DSS colitis injury on RNA expression levels of stem (Lgr5 and Krt19) and progenitor (Atoh1 and Notch1) cell markers. Lgr5 mRNA expression is significantly decreased after exposure to DSS (N=6-7 per condition). Data are represented as mean ± SEM analyzed using Student's t-test. *P≤0.05, ** P≤0.01."
NCBI gene IDs:
"Atoh1: 11921
Krt19: 16669
Lgr5: 14160
Notch1: 18128"
Figure legend: "(k) The extent of epithelial damage is similar in mice treated with DSS alone versus DSS+DT (to ablate Lgr5+ stem cells) (N=3 DSS; N=6 DSS+DT). Data are represented as mean ± SEM analyzed using Student's t-test. *P≤0.05, ** P≤0.01."
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "(l) RNAscope for Lgr5 in Lgr5DTR-GFP mice showing loss of Lgr5 transcripts after DT treatment (N=3-4 per condition). Data information: Nuclei are counterstained with Dapi (blue); white dashed lines indicate basement membrane. Scale bars =50µm"
NCBI gene IDs:
"GFP: 
DTR: 15200
Lgr5: 14160"
Figure legend: "DT ablation of Krt19+ cells significantly reduced the number of lineage labeled crypts (N≥3 per condition). Data are represented as mean ± SEM using Student's t-test *P≤0.05, **P≤0.01."
NCBI gene IDs:
"Krt19: 16669"
Figure legend: "Images of tissue sections Notch1CreERT2; ROSA26tdTomato;Lgr5DTR-GFP mice (f) showing Notch1 lineage labeled crypts. No partially labeled crypts were found after Lgr5+ stem cell ablation. Data information: Nuclei are counterstained with Dapi (blue); white dashed lines indicate basement membrane. Scale bars=50μm."
NCBI gene IDs:
"GFP: 
Cre: 2777477
ROSA26: 14910
DTR: 15200
Lgr5: 14160
ERT2: 26417
Notch1: 18128"
Figure legend: "(d-e) Flow cytometry for tdTomato+ cells in crypt epithelium from DT treated Atoh1CreERT2;Lgr5DTR-GFP;ROSA26tdTomato mice."
NCBI gene IDs:
"GFP: 
tdTomato: 
Atoh1: 11921
Cre: 2777477
ROSA26: 14910
DTR: 15200
Lgr5: 14160
ERT2: 26417"
Figure legend: "(f) tdTomato+ colonic organoids from single Atoh1-tdTomato+/Lgr5-negative cells (N=1; n=3 technical replicates per condition). Data information: Scale bars =100μm"
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "(h) tdTomato+ colonic organoids established from mice in which Lgr5+ stem cells have been ablated show higher growth efficiency compared to their colonic organoid counterparts (N=1; n=3 technical replicates per condition). Data are represented as mean ± SD analyzed using two-way ANOVA with Sidak's multiple comparisons test (h). *P≤0.05, **P≤0.01, ***P≤0.001."
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "C: The infectivity  of PIPLC  and PK-treated cell lysates was determined by infecting CAD5  cells with lysates as indicated. The signal intensity of the highest dilution was measured and compared to untreated RML CAD5  cell lysate. PIPLC-treated cells and PK-treated lysates showed similar infectivity  titers. Prion-infected and NBH, as well as RML on CAD5ΔPrnp were used for control."
NCBI gene IDs:
"Prnp: 19122"
Figure legend: "D: RML-infected CAD5  and GT-1/7  cells were transfected with non-targeting (NT) or Prnp  targeting siRNAs in a 384-well plate and subjected to PK    or PIPLC   treatment. Z'-factors were calculated for each condition. Shown are mean ± SD, n = 22 individually cultured wells."
NCBI gene IDs:
"Prnp: 19122"
Figure legend: "A: Western blot showing Hnrnpk siRNA transfection (96 hrs.) decreases Hnrnpk protein levels while increases PrPSc in RML prion infected GT-1/7. Prnp siRNAs suppressed both PrPC and PrPSc as expected. ⍺: anti Quantifications are reported as normalized to Actin and in comparison, to NT. PK-western blot is quantified relative to NT."
NCBI gene IDs:
"Hnrnpk: 15387
Prnp: 19122"
Figure legend: "C: mRNA levels of Hnrnpk  and Prnp  following siRNA and PSA  treatment. FPKM: Fragments per kilobase of transcript per million mapped reads. Hnrnpk  siRNAs lead to a decrease in Hnrnpk  mRNA levels as well as an increase in Prnp  mRNA levels. No difference is seen between DMSO  treated and PSA  treated cells for either Hnrnpk  levels of Prnp  levels. n = 2 per treatment group."
NCBI gene IDs:
"Hnrnpk: 15387
Prnp: 19122"
Figure legend: "E: Brightfield microscopy  images of the effect of siRNA mediated HNRNPK  downregulation on prion-induced vacuolation  in PG127 hovS   cells. Downregulation of HNRNPK  leads to an enhanced cytopathological vacuolation  phenotype when compared to NT siRNA. ovPRNP siRNA transfected, as well as uninfected cells were used as controls Downregulation of ovPRNP in the infected hovS   eliminates the vacuoles; Right panel: Quantification of vacuoles  of NT, HNRNPK  and ovPRNP siRNA treated PG127  hovS. Cells from pictures at three different positions in the well were manually counted and the amount of vacuolated cells was normalized to the total amount of cells. Values represent mean ± SD. * p = 0.0113, **** p = &lt;0.0001 (Dunnett's multiple comparisons test). Scale bar = 100 µm. n = 3 technical replicates."
NCBI gene IDs:
"HNRNPK: 3190
PRNP: 493887"
Figure legend: "G: Same as in E, using shRNAs instead of siRNA. Downregulation of HNRNPK  leads to an enhanced cytopathological vacuolation  phenotype when compared to NT shRNA. Uninfected cells were used as controls Right panel: Quantification of vacuoles  of NT and HNRNPK  shRNA treated PG127 hovS. Cells from pictures at three different positions in the well were manually counted and the amount of vacuolated cells was normalized to the total amount of cells. Values represent mean ± SD. *** p = &lt;0.0009 (Dunnett's multiple comparisons test). Scale bar = 100 µm. n = 3 technical replicates."
NCBI gene IDs:
"HNRNPK: 3190"
Figure legend: "H: Quantification of PrPSc  levels in PG127 hovS  cells following treatment with shRNA against HNRNPK  and 1 µM PSA  in comparison to DMSO  and NT. Each dot represents an experiment. *= p-value &lt; 0.027 (Student's t-test). Shown are mean ± SD."
NCBI gene IDs:
"HNRNPK: 3190"
Figure legend: "I: Gene set overrepresentation analysis of differentially expressed genes (log2FC -0.25 ≥ or 0.25 ≤ and FDR ≤ 0.05) for siRNA mediated Hnrnpk  downregulation or PSA  treatment in RML GT-1/7 cells analyzed by  RNAseq. Differentially regulated genes (up in siRNA treatment and down in PSA  or vice versa) were overlapped and used for pathway analysis. No significantly enriched pathways are detected for upregulated genes in Hnrnpk  and downregulated in PSA  treatment. For the opposing direction, an enrichment of genes involved in glucose  metabolism was detected."
NCBI gene IDs:
"Hnrnpk: 15387"
Figure legend: "(F) Stills from time-lapse  sequence of representative  Hela cells expressing  mNeon-Green Aurora B  and Cell Mask to label cell membrane, exiting mitosis, showing the dynamic  re-localization of Aurora B  from DNA  to the overlapping  microtubules and cleavage furrow with control siRNA treatment (arrows top). With knock-down of RACGAP1, Aurora B still re-localises from the DNA to the microtubules and furrow  in the midzone in early anaphase (arrows bottom), but the microtubule localization is lost at later stages (asterisk bottom). Scale bar - 10 µm."
NCBI gene IDs:
"RACGAP1: 29127"
Figure legend: "(E) Stills of representative Hela  cells expressing  Lifeact-GFP and  H2B-mCherry   at 10 mins following forced mitotic exit with siControl, siRACGAP1, DMSO  and 2µM ZM447439 treatment. Control siRNA, RACGAP1  siRNA and DMSO  treated cells show clearance  of  actin from the cortex  close to the DNA, while ZM447439 treated cells fail to clear  actin, quantified in (F). Note, failure in  actin  accumulation in RACGAP1 depleted cell, asterisk. Scale bar - 10 µm"
NCBI gene IDs:
"RACGAP1: 29127"
Figure legend: "(F) Quantification of  actin    intensity across the perimeter  of the cells based on distance from DNA, with 5 and 6 being closest to DNA and 1 and 10 furthest away, as in cartoon (B). Graph shows decrease in  actin intensity closer to the DNA in siControl (n=26), siRACGAP1 (n=37) and Control DMSO  treated cells (n=38 cells), seen by levels lower than 1 (dotted red line), while this decrease is absent in  ZM447439 treated cells (n=42 cells). Data presented as mean ± SD."
NCBI gene IDs:
"RACGAP1: 29127"
Figure legend: "(D) Still images of representative  Hela  cells expressing Lifeact-GFP and H2B-mCherry captured at early anaphase  under different conditions i.e. Control siRNA, knock-down of centralspindlin  protein RACGAP1, in the presence and absence of  Aurora B kinase inhibitor (2µM ZM). Scale bar - 10 µm (E) Quantification of aspect ratio of cells treated with different conditions as in (D) at 6 mins after anaphase onset, before furrow formation. Aurora B inhibition and siRACGAP1 individually have a moderate effect on aspect ratio, while together have a stronger impact during mitotic exit. In combined treatment, mitotic cells hardly undergo any elongation and remain spherical before re-spreading. Ordinary one-way ANOVA with multiple comparison. Each treatment is compared with siControl, ****p&lt;0.0001 for all comparisons. Data represented as box-whisker plot, with box showing 25th-75th percentile values, whiskers-min to max value and line representing median. Mean±SD for different treatments- siControl (n=38) - 1.455±0.19, ZM  (n=32) - 1.219±0.16, siRACGAP1  (n=25) -1.144±0.078 and siRACGAP1+ZM (n=16) - 1.013±0.03. Cells for different treatment pooled from n&gt;3 independent experiments. "
NCBI gene IDs:
"RACGAP1: 29127"
Figure legend: "(C) Quantification of actin  intensity at the poles of cells at 6 mins post anaphase onset, before furrow  formation under different conditions as in (A). Actin fails to be cleared from the poles upon Aurora B kinase inhibition during mitotic exit and is stronger when both centralspindlin  and Aurora B kinase  activity are affected, while it is cleared upon RACGAP1 depletion. Data represented as mean±SD for treatments - siControl- 0.98±0.05, siRACGAP1  - 0.99±0.04, ZM - 1±0.039, siRACGAP1+ZM - 1±0.037. Ordinary one-way ANOVA with multiple comparison- siControl vs siRACGAP1 - n.s., p=0.896, siControl vs  ZM - **p=0.0018, siControl vs siRACGAP1+ZM - ****p&lt;0.0001 and ZM  vs siRACGAP1+ZM - p=0.1528."
NCBI gene IDs:
"RACGAP1: 29127"