EMBO/SourceData huggingface dataset:
Figure legend: "F. Defective cilia  formation occurs in ccdc108 CRISPR  mutants. Embryos  at one cell stage were injected with Cas9 protein with or without the sgRNA against  ccdc108 (108sg) and fixed at stage 27. Representative 3D-SIM images and plot show significantly reduced cilia  number in  ccdc108 CRISPR  mutants. &gt; 70  MCCs  from 6 embryos for each condition. Cell membranes (mGFP, purple), cilia (Ac-tub, green) and basal bodies  (Cep164, yellow) were labeled with indicated antibodies. Data information: Quantification data were collected from three independent experiments. Unpaired two-tailed t-test was performed (*** p&lt;0.001). Mean ± s.d. values are presented."
NCBI gene IDs:
"ccdc108: 
CRISPR: "
Figure legend: "G.  In situ hybridization chain reaction (HCR) reveals that ccdc108  mRNA is specially detected in  MCCs. Embryos  at stage 35 were fixed and subjected to in situ HCR (red). Embryos were then incubated with the  Ac-tub   antibody (cyan) and DAPI (gray)."
NCBI gene IDs:
"ccdc108: "
Figure legend: "C, D. Representative confocal  images and plots show that  ccdc108 morphants displayed  ciliogenesis defects in neuromasts (C) and olfactory placodes (D). Zebrafish embryos were fixed and stained  with the Ac-tub  antibody (green),  phalloidin (purple) and Hoechst  (blue). Data information: Quantitative data were from three independent repeats. Unpaired two-tailed t-test was performed (*** p&lt;0.001; * p&lt;0.05). Mean ± s.e.m values are presented."
NCBI gene IDs:
"ccdc108: 100333283"
Figure legend: "E.  Scanning electron microscope images of zebrafish embryos display a failure in cilia  formation in Ccdc108 depleted olfactory placodes."
NCBI gene IDs:
"Ccdc108: 100333283"
Figure legend: "H. Representative 3D-SIM images and plot reveal that depletion of CCDC108 leads to a significant reduction in multiciliogenesis in mEPCs. mEPCs infected with  lentivirus  expressing indicated shRNA were  serum starved for five days and subjected to immunostaining. GFP-CETN1  (blue) marked mEPCs infected with  lentivirus. Basal bodies (CEP164, purple) and cilia (Ac-tub, green) were labeled with indicated antibodies. &gt; 60 MCCs from three independent repeats for each condition were counted. Data information: Quantitative data were from three independent repeats. Unpaired two-tailed t-test was performed (*** p&lt;0.001; * p&lt;0.05). mean ± s.d. values are presented."
NCBI gene IDs:
"CCDC108: 241116"
Figure legend: "A. Depletion of  Ccdc108  causes disorganized basal body distribution in MCCs. Representative 3D-SIM images (x-y) and 3D-reconstructions (x-z) of MCCs of embryos  show a failure of apical trafficking of basal bodies  in Ccdc108  depleted cells. Cell membranes (mGFP, blue), basal bodies (Cetn1, green) and distal appendages  (Cep164, purple) were labeled with indicated antibodies. Select area of  Cep164 channel was zoomed to show centriolar Cep164  accumulations. Orange arrowheads and numbers mark individual centriole. &gt; 70 MCCs  from 6 embryos for each condition were counted. Data information: Quantitative data were from three independent experiments. Unpaired two-tailed t-test was performed (*** p&lt;0.001 ; ** p&lt;0.01; * p&lt;0.05). Mean ± s.d. values are presented."
NCBI gene IDs:
"Ccdc108: "
Figure legend: "B.  Transmission electron microscopy images of Xenopus embryos displaying aberrant apical  trafficking and docking of centrioles in Ccdc108 depleted MCCs. Orange arrowheads mark centrioles. Percentage of  centrioles  docked to plasma membrane  in each field were scored. At least 7 cells for each sample were counted."
NCBI gene IDs:
"Ccdc108: "
Figure legend: "C. Representative  3D-SIM images reveal that depletion of Ccdc108 has no effect on apical expansion in MCCs. Embryos were fixed and stained with phalloidin (purple). Cilia (Ac-tub, gray) were also presented.  Apical  area of MCCs in each condition were measured ; 80  MCCs from 6 embryos for each condition. D. Representative confocal  images show a significant reduction in apical actin following depletion of    Ccdc108. Cell boundaries and apical actin   network were marked with mGFP (green) and phalloidin (purple) and phalloidin intensity levels measured and plotted. &gt; 80 MCCs from 6 embryos  for each condition. Data information: Quantitative data were from three independent experiments. Unpaired two-tailed t-test was performed (*** p&lt;0.001 ; ** p&lt;0.01; * p&lt;0.05). Mean ± s.d. values are presented."
NCBI gene IDs:
"Ccdc108: "
Figure legend: "A-C. The IFT-interaction domain is required for Ccdc108 multiciliogenesis function in Xenopus embryos: (A) impaired  multiciliogenesis, (B) defective apical migration/docking of centrioles, and (C) reduced enrichment of apical F-actin. Embryos at stage 27 were fixed and stained with the acetylated tubulin (A; Ac-tub; green), Cep164 (B; purple) and  Cetn1 (B; green) antibodies or phalloidin (purple). mRNA of a membrane-bound form of GFP was co-injected with each morpholino to indicate targeted cells (A,B). Typical D-SIM images (A,B) and confocal  images (C) are present. Embryos were treated as described in Figure 1C and expressed mGFP or  GFP-Ccdc108 mutants. Plots show results from three independent experiments. (A) &gt; 70 MCCs from 6 embryos, and (C) &gt; 70 MCCs from 6  embryos for each condition. Unpaired two-tailed t-test was performed (*** p&lt;0.001; **p&lt;0.01). Mean ± s.d. values are presented."
NCBI gene IDs:
"Ccdc108: 
GFP: 
mGFP: 
Ccdc108: 100144742"
Figure legend: "A, ift74 CRISPR  mutants display defective cilia formation. Embryos  at  one cell stage were injected and fixed at stage 27 with two independent sgRNA targeting  ift74. Representative confocal images of the  Xenopus epidermis, and plots display defective ciliation. ;60 MCCs from 6  embryos Cell membranes (mGFP), cilia (Ac-tub, green) were labeled with indicated antibodies. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"CRISPR: 
ift74: 734317"
Figure legend: "B. ift74 CRISPR  mutants display defective  cilia  formation. Embryos at one cell stage were injected and fixed at stage 27 with two independent sgRNA targeting ift74. Representative  3D-SIM  images of the  Xenopus epidermis, and plots display defective ciliation. ;70 MCCs from 6 embryos for each condition. Cell membranes (mGFP), cilia (Ac-tub, green) and  basal bodies (Cep164) were labeled with indicated antibodies. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"CRISPR: 
ift74: 734317"
Figure legend: "C. Ift74  depletion affects centriole apical migration in MCCs. Representative 3D-SIM images (x-y) and 3D-reconstructions (x-z) of MCCs show failure of apical trafficking of basal bodies. Basal bodies (Cetn1, green) and distal appendages  (Cep164, purple) were labeled with indicated antibodies. Quantitative plot shows a slight significant reduction in  basal bodies by the depletion of Ift74. &gt;70  MCCs from 6 embryos for each condition. D. Representative 3D-SIM images and plots reveal that apical  expansion of MCCs is slightly affected by Ift74  depletion. &gt;70 MCCs  from 6 embryos  for each condition. Embryos  were fixed and stained with phalloidin (purple). Cilia (Ac-tub, gray) were also presented. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"Ift74: 734317"
Figure legend: "E. Representative confocal  images and plot show a significant reduction in  apical F-actin  enrichment upon Ift74 depletion. &gt;70 MCCs  from 6  embryos for each condition.  Cell boundaries and apical actin network were marked with mGFP (green) and phalloidin (purple). Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"Ift74: 734317"
Figure legend: "C-E. Ccdc108 depletion affects  centriolar distribution of IFT-B proteins during  centriole migration in MCCs. Embryos were co-injected with GFP-IFTs (C: Ift74; D:  Ift80; E:  Ift88; green), Cetn4-RFP (purple) and each  morpholino, and imaged at stage 18.  HA  tagged Ccdc108  proteins were co-expressed to test for the rescue of phenotypic defects. Xenopus embryos were subjected to  immunoblotting to evaluate the protein expression levels. α-tubulin (α-Tub) served as loading control. Quantitative plots show that  Ccdc108-depleted MCCs showed a significant decrease of centriolar  distribution of IFT-B proteins (Ift74, Ift80 and Ift88), and Ccdc108-IFT  interaction deficient mutants are unable to rescue the decreased centriolar accumulation of IFT-B proteins. &gt;40 MCCs from 6 embryos for each condition. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001). Mean ± s.d. values are also presented."
NCBI gene IDs:
"Ccdc108: 
GFP: 
Ift74: 734317
Ift80: 394395
Ift88: 8100"
Figure legend: "A, B. Centriolar distribution of ciliary adhesion complex related proteins  Fak and Cp110  are not reduced following Ccdc108  depletion.  Embryos  were co-injected with GFP-Cp110 (A) GFP-Fak (B),  Cetn4-RFP (purple) and each morpholino, and imaged at stage 18. &gt;30  MCCs from 6 embryos  for each condition. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"Ccdc108: 
GFP: 
Cp110: 108701549
Fak: 432072"
Figure legend: "C, D.  Ccdc108   depletion affects  centriolar distribution of PCP associated actin cytoskeleton regulators important for centriole migration in MCCs. Embryos were co-injected with GFP-Drg1 (C) GFP-RBD (D), Cetn4-RFP (purple) and each morpholino, and imaged at stage 18.  HA tagged Ccdc108 proteins were co-expressed to test for the rescue of phenotypic defects. Xenopus embryos  were subjected to immunoblotting to evaluate the protein expression  levels.  α-tubulin (α-Tub) served as loading control. Quantitative plots show that Ccdc108-depleted MCCs showed a significant decrease of  centriolar  distribution of Drg1 and  RBD, and neither of Ccdc108-IFT interaction deficient mutants can rescue the  centriolar phenotypic defects. &gt;30 MCCs  from 6 embryos for each condition. Data information: Quantitative data from three independent experiments were scored. Unpaired two-tailed t-test was performed (*** p&lt;0.001; ** p&lt;0.01). Mean ± s.d. values are also presented."
NCBI gene IDs:
"Ccdc108: 
GFP: 
RBD: 
Drg1: 399253"
Figure legend: "I. EATR assay. Chondrocytes with indicated genotypes (ctrl=wild type) treated with FGF18 (50 ng/mL; 16 hours) where indicated. ER acidification was measured by FACS. Mean +/- standard error of the mean (sem) of N= 17 (veh), N=17 (FGF18), N=4 (FGFR3;4KO); N=4 (FGFR3;4KO FGF18) biological replicates. Analysis of variance one way (ANOVA) p&lt;0.0001; Tukey's post hoc test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"FGFR3: 84489"
Figure legend: "E. Co-immunofluorescence of p-IRS1 S307 mouse (green) and p-S6 S240/S242 (red) ribosomal protein in IRS1-overexpressing RCS chondrocytes treated with FGF18 (50 ng/mL) for 12h. Nuclei (N) were stained with DAPI (blue). Scale bar 15 μm. Quantification analysis of p-S6 fluorescence intensity in IRS1-overexpressing vs non expressing RCS chondrocytes. Mean +/- standard error of the mean (sem) of N=3 biological replicates. n=35 cells analyzed. Student's paired T-test *p&lt;0.05."
NCBI gene IDs:
"IRS1: 16367"
Figure legend: "E. qRT-PCR analysis of ER-phagy receptors in RCS chondrocytes. Gene expression was analyzed after FGF18 (50 ng/mL) treatment for 16 hours. Fold change values were relative to vehicle (5% ABS) and normalized to Cyclophilin gene. Mean +/- standard error of the mean (sem) of N=3 biological replicates/treatment. Student's paired T-test **p&lt;0.005; *p&lt;0.05."
NCBI gene IDs:
"Cyclophilin: "
Figure legend: "F. (top) Representative model of Fam134b∆LIR protein. LIR= LC3-Interacting-Region. (bottom) Western blot analysis of Fam134b in chondrocytes with indicated genotypes (ctrl=wild type) treated with FGF18 (50 ng/mL) for 16 hours. Representative images of N=3 biological replicates/treatment. β-actin was used as loading control. Bar graph showed quantification of Fam134b band intensity normalized to β-actin. Mean +/- standard error of the mean (sem). Student's paired T-test, *p&lt;0.05."
NCBI gene IDs:
"Fam134b: 619558"
Figure legend: "B. Western blot analysis of TFEB, and phospo-TFEB (Serine 142) in RCS chondrocytes stably expressing human TFEB-3XFlag protein. Cells were treated with vehicle (5% ABS) or FGF18 (50 ng/mL) for 16h. β-actin was used as loading control. Representative of three independent experiments."
NCBI gene IDs:
"Flag: 
TFEB: 7942"
Figure legend: "C. GFP immuno-EM staining in FGFR3/4KO chondrocytes infected with a constitutive nuclear (and active) mutant TFEB fused to GFP tag (TFEB- S142A:S211A-GFP). GFP positive gold immune-particles showed presence of GFP-puncta into the nucleus (N, stained in green for visualization). Lysosomes were stained in blue for visualization. Insets showed magnification of lysosomes. Quantification of lysosome diameter (nm) in control (wild type) and FGFR3;4KO chondrocytes infected with empty or with TFEB- S142A:S211A-GFP vector. Mean +/- standard error of the mean (sem) of N=3 biological replicates. n=40 (veh) n=78 (FGFR3;4KO) n=57 (FGFR3;4KO +TFEB-S142A:S211A-GFP) cells were analyzed. Scale bar 500 nm. Kruskal Wallis test p=1.43e-13; Nemenyi post hoc test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"GFP: 
FGFR3: 84489
TFEB: 7942"
Figure legend: "D. Co-immunofluorescence of p-IRS1 (S307 mouse) and TFEB in IRS1-overexpressing RCS chondrocytes treated with FGF18 (50 ng/mL) for 12h. Nuclei (N) were stained with DAPI (blue) and delimited with dashed line. Scale bar 15 μm. Quantification of TFEB nuclear localization in IRS1-overexpressing vs non-expressing RCS. Mean +/- standard error of the mean (sem) of N=3 biological replicates. n= 124 cells analyzed; Student's un-paired T-test *p&lt;0.05."
NCBI gene IDs:
"IRS1: 16367"
Figure legend: "A. qRT-PCR analysis of Fam134b gene expression in chondrocytes with indicated genotypes (ctrl=wild type) treated with vehicle (5% ABS) or with FGF18 (50 ng/mL; 16 hours). Fold change values were relative to vehicle and normalized to Cyclophilin gene. Mean +/- standard error of the mean (sem) of N=3 biological replicates. Analysis of variance one way (ANOVA) p&lt;0.0001: Tukey's post hoc test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"Cyclophilin: 
Fam134b: 619558"
Figure legend: "C. Co-staining of CLIMP63 (green) and TMEM192-HA (lysosomes, red) in control and TFEB;3KO RCS treated with FGF18 (50 ng/mL for 16 hours). BaFA1 was used at 100 nM for 3h. Scale bar 15 μm and 2 μm (higher magnification boxes)."
NCBI gene IDs:
"TFEB: 316214"
Figure legend: "F,G Data plots show quantification of mCherry+ vesicles/cell (autolysosomes) (F) and mCherry+/GFP+ vesicles/cell (autophagosomes) (G) in wild type (ctrl) and TFEB;3KO cells treated with vehicle (veh) or FGF18. N=3 independent experiments. Mean +/- standard error of the mean (sem) of N=24 (wild type treated with 5%ABS, veh), N=30 (wild type treated with FGF18), N=27 (TFEB;3KO veh), N= 33 (TFEB;3KO FGF18) cells. Student un-paired T-Test ***p&lt;0.0005; **p&lt;0.005."
NCBI gene IDs:
"TFEB: 316214"
Figure legend: "H. ChIP analysis of TFEB binding to Fam134b DNA in RCS cells transfected with TFEB-3XFLAG. Numbers in the CLEAR site (yellow box) refer to the distance [in base pairs] from the transcriptional start site (+1) of Fam134b-2 gene. Immunoprecipitated DNA was normalized to the input and plotted as relative enrichment over a mock control. Bar graph shows fold change enrichment; mean +/- standard error of the mean (sem) of N=3 independent experiments. Student un-paired T-Test **p&lt;0.005."
NCBI gene IDs:
"FLAG: 
Fam134b: 619558
TFEB: 7942"
Figure legend: "I-J. Luciferase assays in RCS chondrocytes using as promoter a 0,7kb genomic Fam134b DNA fragment containing a wild type (FAM134B-WT) or a deleted (FAM134B-mut) version of the CLEAR site. TFEB plasmid transfection amount and FGF18 (50 ng/mL for 16h) treatments are indicated. Mean +/- standard error of the mean (sem) of N=3 biological replicates. Student paired T-test *p&lt;0.05; **p&lt;0.005."
NCBI gene IDs:
"Fam134b: 619558
FAM134B: 619558
TFEB: 7942"
Figure legend: "K. Luciferase activity in wild type (ctrl) and TFEB;3KO RCS chondrocytes with indicated genotypes expressing the indicated Fam134b luciferase report plasmids and treated with FGF18 overnight (50 ng/mL) where indicated. Mean +/- standard error of the mean (sem) of N=5 biological replicates. Analysis of variance one way (ANOVA) p&lt;0.0001: Sidak's multiple comparison test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"Fam134b: 619558
TFEB: 316214"
Figure legend: "A. qRT-PCR analysis of Fam134b isoforms in mock, TFEB S142A:S211A and TFE3 S246A:S312A over-expressing U2OS cells. Values were normalized to Hprt gene and expressed as fold change values relative to mock. Mean +/- standard error of the mean (sem) of N=3 biological replicates. Student's un-paired T-test *p&lt;0.05; NS not significant."
NCBI gene IDs:
"Hprt: 3251
Fam134b: 54463
TFE3: 7030
TFEB: 7942"
Figure legend: "B. Western blot analysis of Fam134b isoforms in mock, TFEB S142A:S211A and TFE3 S246A:S312A over-expressing U2OS cells showing induction of FAM134B-2, but not of FAM134B-1 isoform by TFEB/TFE3. Filamin A was used as loading control. Blot is representative of N=3 independent experiments."
NCBI gene IDs:
"TFE3: 7030
TFEB: 7942"
Figure legend: "C,D. Co-immunofluorescence staining of ER (CLIMP-63, green) and lysosomes (TMEM192-HA, red) in control and Sh-FAM134B U2OS cells over-expressing TFEB S142A:S211A-GFP (purple). BaFA1 was used at 100 nM for 4h. Scale bar 15 μm and 2 μm (higher magnification boxes) (C). In (D), Quantification of CLIMP63 fluorescence in TMEM192-HA decorated lysosomes. Mean +/- standard error of the mean (sem) of N=3 biological replicates. N= 21 (vehicle ctrl), N=33 (TFEB S142A:S211A-GFP ctrl), N=21 (vehicle shFAM134B), N= 30 (TFEB S142A:S211A-GFP shFAM134B) cells were analysed. Student's un-paired T-test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"GFP: 
FAM134B: 54463
TFEB: 7942"
Figure legend: "E,F. Co-immunofluorescence staining of ER (CLIMP-63, green) and lysosomes (Lamp1, red) in WT and Fam134bKO MEF cells over-expressing TFEB S142A:S211A-GFP (purple). BaFA1 was used at 100 nM for 4h. Scale bar 15 μm and 2 μm (higher magnification boxes) (E). In (F), Quantification of CLIMP63 fluorescence in Lamp1 decorated lysosomes. Mean +/- standard error of the mean (sem) of N=3 biological replicates. N= 20 (vehicle ctrl), N=22 (TFEB S142A:S211A-GFP ctrl), N=21 (vehicle Fam134bKO), N= 20 (TFEB S142A:S211A-GFP Fam134bKO) cells were analysed. Student's un-paired T-test ***p&lt;0.0005; NS not significant."
NCBI gene IDs:
"GFP: 
Fam134b: 66270
TFEB: 7942"
Figure legend: "A,B. Representative images of alcian blue (cartilage) and Alizarin red (bone) skeletal staining showing growth retardation in Fgfr3/4 dKO mice compared to age/sex wild type littermate at post-natal day 30. B. Femur, tibia and tail details."
NCBI gene IDs:
"Fgfr3: 14184"
Figure legend: "C. Haematoxylin/Eosin staining of femoral growth plate sections from wild type and Fgfr3/4 dKO mice. Higher magnification insets showed a disorganized hypertrophic chondrocyte layer, in Fgfr3/4 dKO mice. Scale bar 60 μm."
NCBI gene IDs:
"Fgfr3: 14184"
Figure legend: "F. qRT-PCR analysis of Fam134b-2 expression from growth plate of mice with indicated genotypes. N=8 (wt mice) and N=9 (Fgfr3/4 dKO mice) were analyzed. Values were normalized to Hprt gene and expressed as fold change relative to control. Mean +/- standard error of the mean (sem). Student's un-paired T-test *p&lt;0.05."
NCBI gene IDs:
"Fgfr3: 14184
Hprt: 15452
Fam134b-2: 66270"
Figure legend: "G. Western blot analysis of Fam134b-2 protein from growth plate lysates of mice with indicated genotypes. β-actin was used as loading control. N=6 (wt mice) and N=4 (Fgfr3/4 dKO mice) were analyzed. Bar graph showed quantification of Fam134b-2 normalized to β-actin. Mean +/- standard error of the mean (sem). Student's un-paired T-test *p&lt;0.05."
NCBI gene IDs:
"Fgfr3: 14184"
Figure legend: "H. Representative immunofluorescence staining of CLIMP-63 of femur growth plate sections from mice with indicated genotypes. Scale bar 10μm. Insets showed increased CLIMP-63 staining in Fgfr3/4 dKO mice. Scale bar 5 μm. Hypert= hypertrophic chondrocytes; pre-hypert= pre-hypertrophic chondrocytes; prolif = proliferating chondrocytes."
NCBI gene IDs:
"Fgfr3: 14184"
Figure legend: "A. Scatter plot of cellular compartments and biological processes regulated by FGF18 in a Fam134b-dependent manner. Score: Student's T-test difference between wild type and Fam134b∆LIR cells. N=3 biological replicates/treatment/genotype were analysed. FDR &lt;0.05."
NCBI gene IDs:
"Fam134b: 66270"
Figure legend: "B. Tandem mass tag secretome analysis of chondrocytes with indicated genotypes and treatments. Fam134b-dependent secreted proteins are shown N=3 biological replicates/treatment/genotype were analysed. FDR &lt;0.05."
NCBI gene IDs:
"Fam134b: 66270"
Figure legend: "C. qRT-PCR analysis of Fam134b and Lamp1 from medaka fish embryos treated with FGF18 (50 ng/mL) for 24h. Values were normalized to Hprt gene and expressed as fold change relative to untreated fish. N= 5 biological replicates. Mean +/- standard error of the mean (sem). Paired Student's T-test **p&lt;0.005; *p&lt;0.05."
NCBI gene IDs:
"Hprt: 
Lamp1: 101175550
Fam134b: 101158885"
Figure legend: "F. Western blot analysis of type II procollagen from a pool of medaka fish embryos with indicated genotypes, showing procollagen accumulation in Fam134bmo fish. β-actin was used as loading control. Bar graph shows quantification of type II procollagen normalized to β-actin. N=3 biological replicates. Mean +/- standard error of the mean (sem). Student's un-paired T-test *p&lt;0.05."
NCBI gene IDs:
"Fam134b: 101158885"
Figure legend: "G. Representative TEM images of medaka fish chondrocytes. Arrows indicated the ER. Insets show ER enlargement in Fam134bmo chondrocyte. N=3 fish/genotype were analyzed. Bar graph shows number of ER enlargement per cell in medaka fish with indicated genotypes. N=29 cells (Fam134bwt) and N=37 cells (Fam134bmo) were analyzed. Mean +/- standard error of the mean (sem). Student's un-paired T-test ***p&lt;0.0005. Scale bar 500 nm. N=nucleus, ER= Endoplasmic Reticulum."
NCBI gene IDs:
"Fam134b: 101158885"
Figure legend: "H. Lateral (top) and ventral (bottom) projections of medaka fish embryos with indicated genotypes. Scale bar 1mm. Bar graphs show quantification of total length and head size of medaka fish model of Fam134bmo expressed as % relative to the scramble. Mean +/- standard error of the mean (sem) of at least n=8 fish/genotype. Student un-paired T-Test *p&lt;0.05."
NCBI gene IDs:
"Fam134b: 101158885"
Figure legend: "Alcian blue (cartilage) (I) staining of scramble and Fam134bmo medaka fish. Graph shows quantification of Ethmoid plate (EP), Palatoquadrate (PQ), Ceratohyal (CH), Paired Prootics (PO), Ceretobranchials 1 to 5 (CB1 to CB5) cartilage length (I) in Fam134bmo and scramble fish. Values were expressed as % relative to the scramble (100% red dotted line). Mean +/- standard error of the mean (sem) of n=9 fish/genotype. Student unpaired T-Test *p&lt;0.05; **p&lt;0.005; ***p&lt;0.0005. NS not significant."
NCBI gene IDs:
"Fam134b: 101158885"