EMBO/SourceData huggingface dataset:
Figure legend: "A. ReDDM activation in Dronc heterozygous (+/-) intestine 7 days after temperature shift at 29 Co; esg expression (green) labels the intestinal progenitor cells, Histone-RFP (red) is a semi-permanent marker retained in differentiated cells and Pdm-1 (grey) labels differentiated ECs. The red arrows indicates the enlarged areas depicted in the insets in the entire figure. DAPI labels the DNA In the entire Figure (blue). All of the experiments in the entire figure have been made under experimental conditions that protect the epithelial integrity (intestine reared in Oxford Medium and flies transferred every two days into vials with fresh food)."
NCBI gene IDs:
"Dronc: 39173"
Figure legend: "B. ReDDM activation in a Drosophila intestine in which all of the progenitor cells are fully mutant for Dronc (see full genotype in methods); note the increased cell size of esg-expressing cells (GFP+, green), the co-expression of Histone-RFP (red) and the EC marker Pdm-1 (gray), and the coexpression of Pdm-1 and GFP in enlarged cells (compare panels and detailed insets from A with B). The image is a representative example of a Drosophila adult gut 7 days after temperature shift at 29 Co. Genotype: w1118; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry"
NCBI gene IDs:
"w1118: 
APEX: 
Cherry: 
Gal4: 
GFP: 
HA: 
TubG80ts: 
Dronc: 39173
CD8: 5740521"
Figure legend: "C. Relative number of esg-expressing cells normalised to DAPI; notice that the relative percentage of esg-labelled cells is significantly higher in Dronc fully mutant conditions (-/-) at 3 days (***; P = 0.0007) and 7d (****; p &lt;0.0001) post temperature shift at 29 Co (Quantifications were made using N ≥ 2 biological replicates; unpaired two-tailed t-test, +/- n=32, -/- n=25)."
NCBI gene IDs:
"Dronc: 39173"
Figure legend: " D. Average cell size of esg-expressing cells (μm²); note the increased cell size of Dronc fully mutant progenitor cells (-/-) at 3d (**; P = 0.0014) and 7d (***, P = 0.0007) post temperature shift at 29 Co (Quantifications were made using N ≥ 2 biological replicates; unpaired two-tailed parametric t-test, 3d +/- n = 19, 3d -/- n = 28, 7d +/- n = 29, 7d -/- n = 28). "
NCBI gene IDs:
"Dronc: 39173"
Figure legend: "E. Relative number of esg-negative cells expressing Histone-RFP normalised to DAPI; notice that the number of Histone-RFP cells without esg expression is significantly higher in Dronc fully mutant (-/-) conditions at 7d (*; P = 0.0046) (Quantifications were made using N biological replicates ≥ 2; Mann-Whitney test, +/- n = 24, -/- n = 20."
NCBI gene IDs:
"Dronc: 39173"
Figure legend: " . A 7d intestine Dronc heterozygous (+/-) intestine in which intestinal stem cells express GFP under the regulation of Dl-Gal4. All of the experiments described in the figure were performed in Oxford medium following an experimental regime that protects the epithelial integrity (flies transferred every two days into vials with fresh food). DAPI (blue) labels the DNA in the entire Figure. Genotype: w1118; ;delta-Gal4 UAS-GFP TubG80ts UAS-Histone-RFP DroncKO/+ (recombinant chromosome made for this study; the parental line delta-Gal4 UAS-GFP TubG80ts UAS-Histone-RFP was obtained from Maria Dominguez) B. A 7d intestine in which the Intestinal Stem Cells have become Dronc homozygous mutant (-/-) upon expressing UAS-Flp under the regulation of Dl-Gal4. Dl-expressing cells are labelled with UASGFP (green); there are no morphological differences between A and B. Genotype: w1118; ;delta-Gal4 UAS-GFP TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry C. Relative number of GFP+ Delta-expressing cells normalised to DAPI; there is no significant increase in intestinal stem cell number between heterozygous and homozygous Dronc mutant conditions (ns; P = 0.9231) (Quantifications were made using N ≥ 2 biological replicates; unpaired two-tailed t test, +/- n = 22, -/- n = 13). Error bars represent Standard Deviation of the Mean. Genotypes: +/- :w1118; ;delta-Gal4 UAS-GFP TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry -/- : w1118; ;delta-Gal4 UAS-GFP TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry D. Average Delta cell size (μm²); notice that the cell size does not change between heterozygous and homozygous Dronc mutant ISCs (ns; P = 0.9694) (Quantifications were made using N ≥ 2 biological replicates; unpaired two-tailed t test, +/- n = 42, -/- n = 21). Error bars represent Standard Deviation of the mean. Genotypes:"
NCBI gene IDs:
"Flippase: 
Flp: 
APEX: 
Cherry: 
Gal4: 
GFP: 
HA: 
RFP: 
TubG80ts: 
w1118: 
Dronc: 39173"
Figure legend: ". A 7d intestine Dronc heterozygous (+/-) intestine in which intestinal stem cells express GFP under the regulation of Su(H)-Gal4. Genotype: w1118; Su(H)-Gal4 UAS-GFP; TubG80ts UAS-Histone-RFP DroncKO/+ A 7d intestine in which the Enteroblasts have become Dronc homozygous mutant (-/-) upon expressing UAS-Flp under the regulation of Su(H)-Gal4. Su(H)-Gal4-expressing cells are labelled with UASGFP (green) and UAS-Histone-RFP; note the increase in cell size when compared to E and the absence of Histone-RFP cells without GFP signal. Genotype: w1118; Su(H)-Gal4 UAS-GFP; TubG80ts UAS-Histone-RFP DroncKO/ UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry Relative number of Su(H)-Gal4-expressing cells normalised to DAPI; there is no significant increase in intestinal stem cell number between heterozygous and homozygous Dronc mutant conditions (n.s. ; P = 0.5099) (Quantifications were made using N ≥ 2 biological replicates; Mann-Whitney test, +/- n = 30, -/- n = 29). Error bars represent Standard Deviation of the mean. Genotypes: +/- : w1118; Su(H)-Gal4 UAS-GFP; TubG80ts UAS-Histone-RFP DroncKO/+ -/- : w1118; Su(H)-Gal4 UAS-GFP; TubG80ts UAS-Histone-RFP DroncKO/ UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry H. Average Su(H) cell size (μm²), there is a significant increase in Su(H) Dronc null cells (-/-) when compared to the heterozygous control condition (**; P = 0.0064) (Quantifications were made using N ≥ 2 biological replicates; Mann-Whitney test, +/- n = 49, -/- n = 33). Error bars represent Standard Deviation of the mean. Genotypes: "
NCBI gene IDs:
"Flippase: 
w1118: 
APEX: 
Cherry: 
Flp: 
Gal4: 
GFP: 
HA: 
TubG80: 
TubG80t: 
Dronc: 39173"
Figure legend: "A. Representative ReDDM labeling of a Drosophila Dronc heterozygous (+/-) intestine overexpressing an RNAi against Notch for 3 days; note the lack of fully differentiated Histone-RFP cells as EC (red) without esg expression (green, GFP). DAPI staining labels cell nuclei in panels All of the experiments described in the figure were performed in Oxford medium following an experimental regime that protects the epithelial integrity. Genotype: w1118 UAS-Notch-RNAi (Joaquin Navascués); esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / + B. Representative ReDDM labelling of a Drosophila Dronc mutant homozygous (-/-) intestine overexpressing an RNAi against Notch for 3 post temperature shift at 29 oC; note the lack of differentiated Histone-RFP cells as EC (red) without esg expression (green, GFP), as well as the increase of GFP-positive cells (compare A to B). Genotype: w1118 UAS-Notch-RNAi (Joaquin Navascués); esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry C. Quantification of the number of Histone-RFP cells normalised to DAPI (proxy of progenitor cell proliferation obtained from the experiments shown in A and B panels); note the statically significant increase in Histone-RFP positive cells in Dronc homozygous mutant intestines compared to controls (**; P =0.0080) (Quantifications were made using N ≥ 2 biological replicates; unpaired two-tailed t test, +/- n = 8, -/- n = 15). Error bars represent Standard Deviation of the mean. Genotypes: +/-: w1118 UAS-Notch-RNAi; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / + -/-: w1118 UAS-Notch-RNAi (Joaquin Navascués); esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry "
NCBI gene IDs:
"Cherry: 
Flippase: 
TubG80ts: 
w1118: 
APEX: 
GFP: 
HA: 
Dronc: 39173
Notch: 31293
CD8: 5740521"
Figure legend: " D. Drosophila Dronc heterozygous intestine overexpressing the Notch intracellular domain for 7 days post temperature shift at 29 oC; intestinal esg-positive progenitor cells (green (GFP) and red (Histone-RFP). Genotype: w1118; esg-Gal4 UAS-CD8-GFP / UAS-Notchintra (Joaquin Navascués); TubG80ts UAS-Histone-RFP DroncKO / + E. Drosophila Dronc homozygous intestine overexpressing the Notch intracellular domain for 7d post temperature shift at 29 oC; notice that the Dronc deficiency accelerates the elimination of intestinal progenitor cells induced by Notch overactivation (compare D and E). The white arrows indicate the position of insets 500μm from the posterior region. Note the complete loss of esg labelled cells in this region. Genotype: w1118; esg-Gal4 UAS-CD8-GFP / UAS-Notchintra; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry F. Relative number of esg-positive cells to DAPI in either heterozygous or homozygous Dronc mutant esg cells overexpressing Notch-Intra; note the significant reduction of esg-expressing cells (****; P &lt; 0.0001) (Quantifications were made using N ≥ 2 biological replicates; Mann-Whitney test, +/- n = 17, -/- n = 17). Error bars represent Standard Deviation of the mean. Genotypes: +/-: w1118; esg-Gal4 UAS-CD8-GFP / UAS-Notchintra ; TubG80ts UAS-Histone-RFP DroncKO / + -/-: w1118; esg-Gal4 UAS-CD8 -GFP / UAS-Notchintra; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry"
NCBI gene IDs:
"Flippase: 
TubG80ts: 
w1118: 
APEX: 
Cherry: 
GFP: 
HA: 
Dronc: 39173
Notch: 31293
CD8: 5740521"
Figure legend: "G. Representative image of a Drosophila Dronc heterozygous intestines 7 days after transfer to 29 oC. esg expression in green (GFP) labels all intestinal progenitors cells, whilst Su(H)-lacZ (red) distinguishes a subpopulation of EBs within the esg-expressing cells. Genotype: w1118, Su(H)GBE-LacZ; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / +"
NCBI gene IDs:
"w1118: 
GFP: 
TubG80ts: 
Dronc: 39173
CD8: 5740521"
Figure legend: "H. Representative image of a Drosophila Dronc KO intestine 7 days after transfer to 29 oC. Note, the transcription of Su(H) in large cells GFP (-), suggestive of an aberrant upregulation of Notch-signalling within fully differentiated ECs. Genotype: w1118, Su(H)GBE-LacZ; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry"
NCBI gene IDs:
"Flippase: 
w1118: 
APEX: 
Cherry: 
GFP: 
HA: 
TubG80ts: 
Dronc: 39173
CD8: 5740521"
Figure legend: "I. Representative image of a 7d Drosophila Dronc heterozygous intestines following a 16hr treatment with paraquat. Note the absence of large Su(H) positive, GFP (-) cells compared to (H). The white arrows indicate the enlarged area depicted in the insets. Genotypes: +/-: w1118, Su(H)GBE-LacZ; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / + -/-: w1118, Su(H)GBE-LacZ ; esg-Gal4 UAS-CD8-GFP / + ; TubG80ts UAS-Histone-RFP DroncKO / UAS-Flippase (BL8209) FRT Dronc-GFP-APEX FRT suntag-HA-Cherry"
NCBI gene IDs:
"Flippase: 
w1118: 
APEX: 
Cherry: 
GFP: 
HA: 
TubG80ts: 
Dronc: 39173
CD8: 5740521"
Figure legend: "B. Growth comparisons of selected kinases with the kinase‑dead mutant match. 24h growth curves (OD595) from liquid culture of yeast strains (W303) expressing human kinase in the presence or absence of 3.2% (v/v) DMSO. Upper panel: BUB1: BUB1 wild type, BUB1-KD: BUB1(K821M); upper middle panel: NEK6: NEK6 wild type, NEK6-KD: NEK6(K74M&amp;K75M); lower middle panel: PKCα-ac: PKCα(A25E), PKCα-KD: PKCα(K368R); lower panel: Control with empty plasmid. Error bars represent SD from three biological replicates."
NCBI gene IDs:
"BUB1: 699
NEK6: 10783
PKCα: 5578"
Figure legend: "C. Y2H interaction experiment with human AAR2 (FL) as bait and PRP8 (aa1755-2335) as prey. Growth on selective media is abolished when AAR2 carries a S284E phospho-mimicry amino acid substitution. Mutation of second candidate phospho-site, AAR2(T356), does not influence the AAR2-PRP8 protein interaction."
NCBI gene IDs:
"AAR2: 25980"
Figure legend: "D. Interaction of AAR2 and PRP8 in human cells. HA‑tagged PRP8 (aa1755-2335) was co-expressed in HEK293 cells with either wildtype AAR2, AAR2(S284A) or AAR2(S284E) mutant versions. Immunoprecipitation of PRP8 led to a co-precipitation of wild typeAAR2 and phospho-null AAR2(S284A). In contrast, the phospho-mimicry AAR2(S284E) version did not bind PRP8."
NCBI gene IDs:
"AAR2: 25980"
Figure legend: "E. Results of the phospho-Y2H screen to identify kinases modulating the interaction of AAR2 and PRP8. Growth on selective media (SD5), of strains carrying AAR2 bait and PRP8 prey plasmids is reduced through co-expression of either SGK2 or CK2α1(CSNK2A1). Growth reduction is visible in a 1:10 dilution series on selective agar (SD5) in comparison to the control media (SD3). Ctrl: non-related kinases."
NCBI gene IDs:
"CK2α1: 1457
CSNK2A1: 1457
SGK2: 10110"
Figure legend: "F. Interaction of AAR2 and PRP8 in human cells in the presences of SGK and CK2α1 kinases. Experiment as in D. Co-expression of wild type SGK2 and CK2α1 reduces the amount of AAR2 co-immunoprecipitated with RPR8."
NCBI gene IDs:
"AAR2: 25980
CK2α1: 1457
SGK: 10110
SGK2: 10110"
Figure legend: "B. Results of the phospho-Y2H screen to identify kinases modulating the interaction of the ERα with 14‑3‑3β. The interaction of ERα (prey) with 14‑3‑3β (bait) is promoted by the co-expression of wildtype kinases of PAK5, CDK17, BUB1 and NEK6, visible by the yeast growth on selective media in comparison to the control (three biological replicas are shown). The coexpression of the kinase‑dead mutants of PAK5 and CDK17 also promoted the interaction of ERα and 14‑3‑3β. In contrast the kinase‑dead mutant versions of BUB1(K821M) and NEK6(K74M&amp;K75M) did not promote the Y2H interaction."
NCBI gene IDs:
"BUB1: 699
CDK17: 5128
NEK6: 10783
PAK5: 57144"
Figure legend: "D. Interaction of ERα and 14‑3‑3β in a GST pull-down assay. GST‑tagged 14‑3‑3β was expressed in E. coli and immobilized on beads. PA‑tagged ERα was co-expressed with either wildtype PAK5 or NEK6, the kinase‑dead (KD) version or without a kinase in HEK293 cells. Immobilized 14‑3‑3β was used to pull PA‑tagged ERα from the HEK lysates. Co-precipitation of ERα was successful in the presence of wild kinases, but not in their absence or with kinase‑dead mutant versions."
NCBI gene IDs:
"NEK6: 10783
PAK5: 57144"
Figure legend: "E. Effect of NEK6 co-expression on ERα gene activation. Relative luciferase activity of ERα in the presence and absence of 1 nM E2 (17β-estradiol) was measured. A dose‑dependent reduction (ng plasmid transfected) in E2-Erα‑dependent luciferase expression was observed in HEK293T, MCF-7 and US-OS cancer cell lines. This effect was not observed with the NEK6(K74M&amp;K75M) kinase‑dead version. Graphs represent ≥ 5 biological replicates, each derived from averaging 6 technical replicates. Error bars indicate SD across the biological replicates."
NCBI gene IDs:
"luciferase: 
ERα: 2099
Erα: 2099
NEK6: 10783"
Figure legend: "B-J: Representative immunofluorescences of the cell types composing the WT and Mecp2 null cultures differentiated from NPCs. Antibodies against Tuj1, Gfap and Olig2 were used to recognize neurons, astrocytes and oligodendrocytes. In D, G and J the percentage of cells positive (mean ± SEM) for each marker was counted over the total of DAPI positive cells (100%). No difference between genotypes was highlighted (Two-way ANOVA); the variation through time resulted significant for the levels of Tuj1 and Gfap (Two-way ANOVA: F(1,16)=9.542, p-value&lt;0.05 and F(1,10)=68.95, p-value&lt;0.0001 respectively). Sample size: n ≥3 wells obtained from two independent preparations; for each well 10 random fields were acquired. Scale bars: 30 µm."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "P: Scatter plots describing the transcriptional levels of selected differentially expressed genes in WT and Mecp2 null cultures differentiated from NPCs and analysed at DIV3 and DIV14. For each gene Two-way ANOVA and Šidák multiple comparison test were used to assess difference between genotypes at the two selected time points (*p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001). Values are represented as mean ± SEM. Sample size: ≥5 samples obtained from two independent preparations."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "Bar graphs represent the expression values (mean ± SEM) of 27 genes, selected for their downregulation in null samples at DIV 14 (p-value &lt;0,07; see Appendix Figure S2), after CX546 administration in the two different time windows (early in G and late in H). One-way ANOVA followed by Dunnett's multiple comparison test was used to compare the expression of each gene between Mecp2 null early and late treated samples and their corresponding untreated controls (set to 1, red dotted line). *: p-value&lt;0.05; **: p-value&lt;0.01, ***: p-value&lt;0.001, ****: p-value&lt;0.0001. Sample size: n≥8 wells deriving from two independent preparations. Each point is representative of a single well."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "I,J: In I One-way ANOVA was used to compare the expression of 9 neuronal genes between Mecp2 null primary neurons (set to 100), null neurons treated with CX546 from DIV1 to DIV4 and null neurons treated with CX546 and Nifedipine from DIV1 to DIV4. * refers to differences between Mecp2 null untreated neurons and Mecp2 null neurons treated with CX546 (*: p-value&lt;0.05; **: p-value&lt;0.01; ***: p-value&lt;0.001); # refers to differences between Mecp2 null neurons treated with CX546 and null neurons treated with both CX546 and Nifedipine (#: p-value&lt;0.05; ##: p-value&lt;0.01). Panel J shows the ratio between the expression level of Kcc2 and Nkcc1 (expressed as 2-∆ct). Box plot shows the median (central band), the lower and upper quartiles (boxes) and the max and min values (whiskers) from at least 3 wells obtained from four different embryos. One-way ANOVA was used to compare the effects of the different treatments on null neurons compared to the untreated ones. **: p-value&lt;0.01. Sample size: n≥3 wells from four different embryos."
NCBI gene IDs:
"Mecp2: 17257
Nkcc1: 20496
Kcc2: 57138"
Figure legend: "B: Kaplan Mayer test shows an improved lifespan of Mecp2 null CX546 treated animals compared to Mecp2 null control animals. Sample size: wt=11, ko=16, ko treated with CX546=11. Gehan-Breslow-Wilcoxon test was used to compare the two groups. **: p-value&lt;0.01."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "(A, B) qRT-PCR analysis of IL6 expression upon indicated stimulations in presence or absence of NOD1 (A) or NOD2 (B). HeLa inducible NOD1 cells (A) or NOD2 cells (B) were induced in absence or presence of doxycycline overnight and afterwards stimulated with various stimuli for 4 h."
NCBI gene IDs:
"IL6: 3569
NOD1: 10392
NOD2: 64127"
Figure legend: "(C) qRT-PCR analysis of Cxcl2 expression upon indicated stimulations in WT, Nod1/2 KO and Rip2 KO bone marrow derived macrophages (BMDMs)."
NCBI gene IDs:
"Cxcl2: 2920
Nod1: 10392
Rip2: 8767"
Figure legend: "(H) qRT-PCR analysis of Cxcl2 expression in WT, Sphk1 KO or Sphk2 KO BMDMs upon indicated stimulations for 4 h."
NCBI gene IDs:
"Cxcl2: 20310
Sphk1: 20698
Sphk2: 56632"
Figure legend: "(D) Western blot analysis of MAPKs and NF-κB activation upon S1P stimulation. HeLa NOD1 or NOD2 cells were treated with cytosolic S1P (20 µM), iE-DAP (20 µM) or MDP (20 µM) for indicated times."
NCBI gene IDs:
"NOD1: 10392
NOD2: 64127"
Figure legend: "Multiplex analysis of CXCL2 (I), CCL5 (J) and IL16 (K) production in supernatants of WT, Nod1/2 and Rip2 KO BMDMs upon S1P (10 µM) or MDP (10 µM) stimulations together with digitonin (2.5 µg/ml) for 20 h."
NCBI gene IDs:
"Nod1: 10392
Rip2: 8767"
Figure legend: "(A, B) Immunoprecipitation with various lipid-coated beads using HeLa NOD1-GFP (A) and NOD2-GFP cells (B). Cell lysates of HeLa NOD1 or HeLa NOD2 cells were incubated with lipid-coated beads at room temperature for 2 h."
NCBI gene IDs:
"GFP: 
NOD1: 10392
NOD2: 64127"
Figure legend: "(F) ELISA analysis of IL8 in supernatants of HEK293T NOD1/2 KO cells transfected with indicated NOD1 or NOD2 mutants. After 24 h transfection, cells were stimulated with S1P (20 µM), iE-DAP (20 µM) or MDP (20 µM) together with digitonin for 20 h."
NCBI gene IDs:
"NOD1: 10392
NOD2: 64127"
Figure legend: "E, Kaplan-Meier  plot depicting metastasis   onset after tumor removal in mice  injected orthotopically with SUM159PT  WT (n = 10), KO (KOd: n = 5; KOs: n = 5) or KO-NNMT  (KOd-NNMT: n= 5; KOs-NNMT: n= 5) cells. *P &lt; 0.05; Log-rank test."
NCBI gene IDs:
"NNMT: 4837"
Figure legend: "F, Bar graph quantification (left panel) and representative bioluminescence images (right panel) of metastases  at day 75 after cancer cell injection of  MDA-MB-231 KO1- RFP  or KO1- NNMT  cells and post-primary tumor removal. n = 7 to 10 animals per group. *P &lt; 0.05; Mann-Whitney U-test. All data are means ± SD. G, Bar graph quantification (left panel) and representative bioluminescence images (right panel) of metastases  at day 34 after injection of MDA-MB-231 KO1- RFP  or KO1- NNMT  cells into the tail veins of mice. n = 10 animals per group. ***P &lt; 0.001; Mann-Whitney U-test. All data are means ± SD."
NCBI gene IDs:
"RFP: 
NNMT: 4837"
Figure legend: "D, Dot plots depicting the overlap of H3K9me3 ChIP-sequencing  and mRNA-sequencing data. In the y axis: differential H3K9me3 enrichment at the gene promoter in NNMT  KO compared to WT cells. In the x axis: differential mRNA expression  of NNMT  KO compared to WT cells. n = 3 experimental replicates. Cut-off: adjusted P &lt; 0.05. Bold dots highlight collagen genes and PRDM5."
NCBI gene IDs:
"NNMT: 4837
PRDM5: 11107"
Figure legend: "F, Bar graph representing average PRDM5  mRNA expression  in  SUM159PT KOs, KOd, and WT cells. n = 3 experimental replicates with 2 technical replicates each. ****P &lt; 0.0001; Mann-Whitney U-test. All data are means ± SD."
NCBI gene IDs:
"PRDM5: 11107"
Figure legend: "G, H, Bar graphs representing average collagen gene mRNA expression  upon overexpression (OE) of PRDM5  in SUM159PT  KOd and KOs cells. n = 3 to 5 experimental replicates with 2 technical replicates each. *P &lt; 0.05, **P &lt; 0.01, n.s., not significant; Kruskal-Wallis test. All data are means ± SD."
NCBI gene IDs:
"PRDM5: 11107"
Figure legend: "E, Bar graphs representing average PRDM5  and collagen gene mRNA expression  upon 5- aza  treatment in SUM159PT  WT, Kos, and KOd cells. n = 3 experimental replicates with 2 technical replicates each. *P &lt; 0.05, n.s., not significant; Kruskal-Wallis test. Data are means ± SEM."
NCBI gene IDs:
"PRDM5: 11107"
Figure legend: "A, Bar graph quantification (left panel) and representative bioluminescence images (right panel) of metastases  at day 27 after injection of  MDA-MB-231 cells into the  tail veins  of mice  bearing short hairpin sh NT (non-targeting), sh1 or sh2 targeting PRDM5. Mice  were fed with doxycycline  food to sustain shRNA expression during the whole experiment. n = 7 to 8 animals per group. **P &lt; 0.01; One-way ANOVA. All data are means ± SD."
NCBI gene IDs:
"PRDM5: 70779"
Figure legend: "B, Box plots depicting bioluminescence quantification (left panel) and representative images (right panel) of metastases  at day 42 and day 62 after intravenous injection of  MDA-MB-231 KO1_RFP, KO1_NNMT1 and KO1_COL1A1 (COL1A1  over-expression) cells. n = 4 to 8 animals per group. **P &lt; 0.01, ***P &lt; 0.001; Day 42: One-way ANOVA, Day 62: Student t-test. Boxes define the upper and lower quartiles; central band indicate the median; whiskers define max to min values."
NCBI gene IDs:
"RFP: 
COL1A1: 1277
NNMT1: 4837"