EMBO/SourceData huggingface dataset:
Figure legend: "I. Time-course of FM4-64 unloading from control, Syt11 KD, and rescued nerve terminals within the same field of view (6 coverslips from at least 3 biological repeats each)."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "A. DRG neurons expressing Myc-Syt11 were immunostained for Myc-Syt11, dynamin (Dyn), AP-2, clathrin (CL), TGN46, synaptobrevin 2 (Syb2), and LAMP1. For dynamin, the box along the plasma membrane was straightened, enlarged, and is shown in the upper right panels with arrows indicating co-localized puncta. An enlarged inset of the inside of the cell (box) is shown in the lower panel. For localization of the transferrin receptor, EGFP-transferrin receptor (TfR) was expressed in DRG neurons. Scale bars, 10 μm."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "A. Transferrin (Tf, red) uptake in Syt11 KD (GFP-positive) and control neurons. Quantitative data are on the right. Scale bars, 20 μm."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "B. Tf uptake in Syt11 over-expressing and control neurons. Quantitative data are on the right. Scale bars, 20 μm."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "C. Normalized ∆Cm induced by 200-ms depolarization of a Syt11 KD neuron in the presence of 100 μM MDC was fitted to a double-exponential decay function (solid black and red, fitted curves)."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "D. Fast and slow time-constants of endocytosis in Syt11 KD neurons recorded as in (C)."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "G, H. Representative z-projected fluorescence images and statistics showing the large dextran uptake in Syt11 KD and control DRG neurons. Neurons were loaded with 50 μM tetramethylrhodamine-dextran (40 kD) by 2-min exposure to 100 mM KCl. Scale bars, 10 μm."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "D, E. Diameters of SVs and DCVs in Syt11 KD and control neurons with (100K, statistics from 6 control cells and 9 KD cells) or without 100 mM K+ stimulation (SE, statistics from 6 cells for each group)."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "Figure 9. Functional domains of Syt11 in endocytosisA. Diagram of the mutant forms of Syt11 used for rescue experiments.B-D. Statistics of FM4-64 (5 min), Tf, and large dextran (40-KD) uptake into KD neurons with or without rescue by indicated forms of Syt11.All data are presented as mean ± s.e.m. of 3 independent experiments. One-way ANOVA, values labeled with different letters (a, b, and c) are significantly different from each other, ***P &lt; 0.001. Student's t-test for the comparison between KD and control cells in (B-D), **P &lt; 0.01, ***P &lt; 0.001."
NCBI gene IDs:
"Syt11: 60568"
Figure legend: "Western blotting in the WT, Trim71 knockout (Trim71_KO), and the Trim71 mutant (R783H) mESCs. The signals from the pluripotency factors Nanog and Oct4 were obtained through short (~10 seconds) exposure."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Distribution of the WT Trim71 and the R783H Trim71 mutant binding regions in the mouse genome. In each CLIP-seq, there are two biological replicates. The binding regions present in both of the biological replicates are used for the analysis."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "CLIP-qRT-PCR for the identified target mRNAs of the Trim71 mutant R783H. The results represent the means (±SD) of three independent experiments."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "UCSC genome browser snapshot for the CLIP-seq data from Trim71 and the Trim71 mutant R783H in the Lsd1 locus. The red box indicates the binding region of the Trim71 mutant R783H. The inputs are from the size-matched input samples in the CLIP-seq analysis."
NCBI gene IDs:
"Lsd1: 99982
Trim71: 636931"
Figure legend: "B. A representative set of Western blots in the WT and the R783H Trim71 mESCs. C. Quantification of the target proteins in the WT and the R783H Trim71 mESCs. Data information: The results represent the means (± SD) of three independent experiments. One-Way ANOVA was used to determine the significance of the difference, *p&lt;0.05. The Western blots listed in B is the Biological Replicate 1"
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Western blotting in the WT and the Trim71 (R783H) mESCs."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Polysome analysis in the WT and the Trim71 (R783H) mESCs."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Quantification of the indicated mRNA distribution in the RNP, 80S, and polysome fractions from the WT and the Trim71 (R783H) mESCs."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Western blotting in the WT mESCs expressing an empty vector, Flag-Trim71, Flag-Trim71(R783H)."
NCBI gene IDs:
"Flag: 
Trim71: 636931"
Figure legend: "Quantification of Lsd1 protein and mRNA in the WT mESCs expressing an empty vector, Flag-Trim71, Flag-Trim71(R783H)."
NCBI gene IDs:
"Flag: 
Trim71: 636931"
Figure legend: "Polysome analysis in the WT mESCs expressing an empty vector, Flag-Trim71, Flag-Trim71(R783H). Quantification of the indicated mRNA distribution in the RNP, 80S, and polysome fractions from the WT mESCs expressing an empty vector, Flag-Trim71, Flag-Trim71(R783H)."
NCBI gene IDs:
"Flag: 
Trim71: 636931"
Figure legend: "Deletion of the Trim71 mutant R783H binding site in Lsd1 mRNA's 3'UTR."
NCBI gene IDs:
"Lsd1: 99982
Trim71: 636931"
Figure legend: "CLIP-RIP followed by qRT-PCR to examine mRNAs associated with the Trim71 and the Trim71 mutant R783H in the WT, Trim71(R783H), CLIP∆, and Trim71(R783H)/CLIP∆ mESCs. The mRNA signals from the E14 mESCs were set as 1 for relative comparison."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Western blotting in the WT, Trim71(R783H), CLIP∆, and Trim71(R783H)/CLIP∆ mESCs."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Quantification of Lsd1 protein and mRNA in the WT, Trim71(R783H), CLIP∆, and Trim71(R783H)/CLIP∆ mESCs. Beta-Tubulin and 18S rRNA were used for normalization in the protein and mRNA quantification, respectively."
NCBI gene IDs:
"Trim71: 636931"
Figure legend: "Western blotting in the WT and the Trim71(R783H) mESCs with dox-inducible expression of Lsd1-GFP."
NCBI gene IDs:
"GFP: 
Lsd1: 99982
Trim71: 636931"
Figure legend: "Western blotting in the WT and the R783H/+ mESCs with dox-inducible expression of Lsd1-GFP."
NCBI gene IDs:
"GFP: 
Lsd1: 99982"
Figure legend: "Exit pluripotency assay for the WT and the R783H/+ mESCs with dox-inducible expression of Lsd1-GFP."
NCBI gene IDs:
"GFP: 
Lsd1: 99982"
Figure legend: "Representative Western blotting and quantification of pluripotency factors during the differentiation of the WT and the R783H/+ mESCs with dox-inducible expression of Lsd1-GFP."
NCBI gene IDs:
"GFP: 
Lsd1: 99982"
Figure legend: "Representative Western blotting and quantification of neural lineage markers during the neural differentiation of the WT and the R783H/+ mESCs with dox-inducible expression of Lsd1-GFP."
NCBI gene IDs:
"GFP: 
Lsd1: 99982"
Figure legend: "Lysis  of different target cells in co-culture with NK  cells was quantified in various  cytotoxicity assays. (B) Left panel: Representative contour plots showing the percentage of  .221-DR4/5KO (control) and .221-Cas9  cells (target) in the presence or absence of NK  cells. Middle panel: Ratio between .221-DR4/5KO and .221-Cas9  cells (y-axis) in the presence or absence of NK  cells. Right panel: Specific lysis  of .221-Cas9  cells displayed as % (n = 12 different donors). Each data point represents the mean of at least 3 technical replicates. Data information: Wilcoxon signed-rank test. Experiments were performed in four batches with three different donors each. \"No NK\" control samples served as a reference for all donors in each batch. Lines connect each data value of the NK  cell condition with their designated \"No NK\" control. Box plots represent the median and 25%/75% percentile. Whiskers indicate minimum and maximum data points."
NCBI gene IDs:
"Cas9: 69900935
DR4: 8797"
Figure legend: "(A-B) qRT-PCR analysis of TGF ligands, TGF receptors, and TGF target expression in control and FRS2 knockdown HASMCs. (NS: not significant compared to control, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001 compared to control; unpaired two-tailed Student's t test). -actin was used for sample loading normalization. Histogram of qRT-PCR results are representative of three independent experiments."
NCBI gene IDs:
"actin: 
FRS2: 10818"
Figure legend: "(C) Left: Immunoblot analysis of TGFRs, phosphorylated Smad2 (p-Smad2), and phosphorylated Smad3 (p-Smad3) in control and FRS2 knockdown HASMCs. Blots are representative of four independent experiments. Right: Band intensities of p-Smad2, p-Smad3, TGFR1, and TGFR2 were normalized to Smad2/3 or GAPDH and expressed as a fraction of a control value. Results are expressed as means ± SD (*p&lt;0.05; ***p&lt;0.001 compared to control; unpaired two-tailed Student's t test)."
NCBI gene IDs:
"FRS2: 10818"
Figure legend: "(A) Left: Immunoblot analysis of smooth muscle marker gene expression in control and FRS2 knockdown HASMCs. Blots are representative of four independent experiments. Right: Band intensities of SM -actin, SM22, and SM-calponin were normalized to -tubulin and expressed as a fraction of a control value. Results are expressed as means ± SD (**p&lt;0.01; ***p&lt;0.001 compared to control; unpaired two-tailed Student's t test)."
NCBI gene IDs:
"FRS2: 10818"
Figure legend: "(B) qRT-PCR analysis of SMC transcription factor gene expression in control and FRS2 knockdown HASMCs. (*p&lt;0.05; **p&lt;0.01; ***p&lt;0.001 compared to control; unpaired two-tailed Student's t test. N=3). -actin was used for sample loading normalization."
NCBI gene IDs:
"actin: 
FRS2: 10818"
Figure legend: "(C) Collagen gel contraction assays were used to determine the contractile ability of control or FRS2 knockdown HASMCs (*p&lt;0.05 compared to control; unpaired two-tailed Student's t test. N=3)."
NCBI gene IDs:
"FRS2: 10818"
Figure legend: "(D-F) Upper panels: Immunoblots of smooth muscle markers, phosphorylated Smad2 (p-Smad2), and TGFR1 expression in control and FRS2 knockdown HASMCs treated with SB431542 (10 m), TGFR2 or Smad2 shRNA lentiviruses. Blots are representative of three independent experiments. Bottom panels: Band intensities of SM-calponin and p-Smad2 were normalized to -tubulin, HSP90, or Smad2 and expressed as a fraction of a control value."
NCBI gene IDs:
"FRS2: 10818
Smad2: 4087
TGFR2: 7048"
Figure legend: "(A) Quantitative real-time PCR analysis of mature let-7 family in control and FRS2 knockdown HASMCs. SNORD47 was used to normalize the variability in template loading. Histogram of qRT-PCR results are three independent experiments."
NCBI gene IDs:
"FRS2: 10818
let-7: RF00027
SNORD47: 26802"
Figure legend: "(B) Upper panel: Immunoblots of SM-calponin, phosphorylated Smad2 (p-Smad2), and TGFR1 expression in control and FRS2 knockdown HASMCs transduced with or without let-7b lentiviruses. Blots are representative of three independent experiments. Bottom panels: Band intensities of SM-calponin and p-Smad2 were normalized to GAPDH or Smad2 and expressed as a fraction of a control value."
NCBI gene IDs:
"FRS2: 10818
let-7: RF00027"
Figure legend: "(D) HASMCs were cultured in the growth medium (M231 + SMGS) at day 0 then switched from growth conditions to differentiation medium (M231 + SMDS) for 8 days. Quantitative real-time PCR analysis of mature let-7 family in HASMCs. SNORD47 was used to normalize the variability in template loading. Histogram of qRT-PCR results are three independent experiments."
NCBI gene IDs:
"let-7: RF00027
SNORD47: 26802"
Figure legend: "(E) Control and FRS2 knockdown HASMCs were cultured in the growth medium (M231 + SMGS) at day 0 then switched from growth conditions to differentiation medium (M231 + SMDS) for 6 days with or without let-7b lentiviruses. Immunoblots of smooth muscle markers, phosphorylated Smad2 (p-Smad2), and TGFR1 expression in control and FRS2 knockdown HASMCs with or without let-7b lentiviruses. Blots are representative of three independent experiments."
NCBI gene IDs:
"FRS2: 10818
let-7: RF00027"
Figure legend: "(A) Dissected mouse aorta demonstrating lipid-rich plaques in brachiocephalic artery after 4 months of high fat diet compared to the normal diet in Apoe-/- mice. (b) &amp; (d) Cross-section of brachiocephalic artery from (a) &amp; (c) stained with Oil Red O. L: lumen. Scale bar: 4 mm. 3 mice per group."
NCBI gene IDs:
"Apoe: 11816"
Figure legend: "(C-F) Analysis of brachiocephalic artery of Apoe-/- mice maintained for 4 months on either normal or high fat diet using anti-CD31 (green), anti-p-FGFR1 (red), anti-FGFR1 (red), anti-p-Smad2 (red), and anti-p-Smad3 (red) antibodies. Nuclei counterstained with DAPI (blue). Scale bar: 62 m. L: lumen. M: Media. 6 mice per group."
NCBI gene IDs:
"Apoe: 11816"
Figure legend: "(A) Representative photomicrographs of Oil Red O-stained atherosclerotic lesions in the aortic arch, of Apoe-/- or Frs2SMCKO/Apoe-/- mice after 16 weeks of high fat diet. 3 mice per group. Scale bar: 5 mm."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(B) (Left) Microphotographs of aortas (en face) from Apoe-/- and Frs2SMCKO/Apoe-/- mice after 16 weeks of high fat diet after staining with Oil Red O. (Right) Lesion area quantification. All data shown as mean ± SD. (***p&lt;0.001 compared to Apoe-/-; unpaired two-tailed Student's t test). 12 mice per group."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(C-D) Representative cross-sections of brachiocephalic arteries Apoe-/- and Frs2SMCKO/Apoe-/- mice stained with hematoxylin and eosin (H&amp;amp;E) (C) and Movat (D). (b)&amp;amp;(d) are high-magnification view from (a)&amp;amp;(c). NC: necrotic core. Apoe-/- mice N=9, Frs2SMCKO/Apoe-/- mice N=12."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(E) Histological analysis of atheosclerotic plaque with anti-Ki67 antibody. Nuclei were counterstained with DAPI (blue). (b)&amp;(d) are high-magnification images from (a)&amp;(c). Scale bar: 62 m (low-magnification images); 10 m (high-magnification images). Apoe-/- mice N=9, Frs2SMCKO/Apoe-/- mice N=12."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(F) Quantification of plaque cellularity; Apoe-/- mice N=9, Frs2SMCKO/Apoe-/- mice N=12 (***p&lt;0.001 compared to Apoe-/-; unpaired two-tailed Student's t test)."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(G) Quantifications of the extent of fibrous cap and necrotic areas in brachiocephalic artery of Apoe-/- and Frs2SMCKO/Apoe-/- mice. (*p&lt;0.05, **p&lt;0.01 compared to Apoe-/-; unpaired two-tailed Student's t test)."
NCBI gene IDs:
"Apoe: 11816
Frs2: 327826"
Figure legend: "(H) Measurement of Ki67+ cells (*p&lt;0.05, ***p&lt;0.001 compared to Apoe-/-; unpaired two-tailed Student's t test)."
NCBI gene IDs:
"Apoe: 11816"
Figure legend: "(C) The degradation of 3HA-Pgc1 was analyzed as in (A) in cells bearing the CDC48 temperature-sensitive allele cdc48-6. Cells were grown 2,5 hours at 37C prior addition of cycloheximide. Inactivation of Cdc48 mutant protein was confirmed by stabilization of the ERAD substrate Erg1 in the same cells."
NCBI gene IDs:
"CDC48: 851431
cdc48: 851431
Cdc48: 851431"
Figure legend: "(A) Localization of chromosomally expressed GFP-Pgc1 from the constitutive ADH1 promoter in wt and doa10Δ cells. Arrowheads indicate GFP-Pgc1 labeling at the ER, which is stained by Sec63-Cherry. LDs were visualized upon staining with the neutral lipid dye MDH. On the bottom, box plot with quantitation of GFP-Pgc1 fluorescence intensity at the nuclear envelope in wt and doa10Δ. GFP-Pgc1 measurements were performed as described in the Materials and Methods section. Scale bar: 5 µm."
NCBI gene IDs:
"ADH1: 854068
doa10: 854781"
Figure legend: "(C) Crude membranes from wt and doa10Δ cells expressing 3HA-Pgc1 were subjected to the indicated treatments and subsequently fractionated into membrane pellet (P) and supernatant (S).(D) Crude membranes from wt and doa10Δ cells expressing 3HA-GFP-Pgc1275-321 were analyzed as in (C)."
NCBI gene IDs:
"Pgc1: 855895
doa10: 854781"
Figure legend: "(A) Localization of GFP-Pgc1 in are1Δ are2Δ lro1Δ dga1Δ cells in presence (no LDs) or absence of DOA10 (no LDs doa10Δ). The ER was visualized by expression of Sec63-Cherry. Scale bar: 5 µm."
NCBI gene IDs:
"are1: 850415
are2: 855753
dga1: 854419
lro1: 855742
DOA10: 854781
doa10: 854781"
Figure legend: "(B) GFP-Pgc1 was expressed under the ADH1 promoter; LD formation was stimulated by galactose-induced expression of DGA1 in are1Δare2Δlro1Δ. Fluorescence microscopy was used to follow GFP-Pgc1 localization over time. LDs were visualized upon staining with the neutral lipid dye MDH. Scale bar: 5 µm."
NCBI gene IDs:
"ADH1: 854068
are1: 850415
are2: 855753
DGA1: 854419
lro1: 855742"
Figure legend: "(C) Photo-conversion of tdEOS-Pgc1 expressed under the ADH1 promoter; LD formation was stimulated by galactose-induced expression of DGA1 in are1Δare2Δlro1Δdoa10Δ cells. The red square marks the photo-converted region. The time (in minutes) after photoconversion is indicated. Arrowheads point LDs containing photoconverted tdEOS-Pgc1. Scale bar 5µm."
NCBI gene IDs:
"ADH1: 854068
are1: 850415
are2: 855753
DGA1: 854419
lro1: 855742
doa10: 854781"
Figure legend: "(D) FRAP experiment of GFP-Pgc1 in wt and doa10Δcells. Representative examples are shown. The bleached areas are marked by red squares and include a LD adjacent to ER. The time (in seconds) after photobleaching is indicated. Each graph shows average fluorescence intensities for 10 cells normalized to pre-bleached plotted over time. Error bars indicate standard deviation."
NCBI gene IDs:
"doa10: 854781"
Figure legend: "(A) Localization of GFP-tagged Pgc1 derivatives in which the hydrophobic hairpin (aa275-321) was replaced by membrane anchor of Scs2 (Pgc1Scs2MA) or Bos1 (Pgc1Bos1MA). ER and LDs were visualized by expression of Sec63-Cherry and MDH staining, respectively. Scale bar: 5 µm."
NCBI gene IDs:
"Bos1: 850767
Pgc1: 855895
Scs2: 856856"
Figure legend: "(B) The degradation of 3HA-Pgc1 or the indicated chimeras in wt and doa10Δ cells was analyzed as in Fig. 1A. The blot is representative of at least three independent experiments."
NCBI gene IDs:
"doa10: 854781"
Figure legend: "(C) The degradation of 3HA-GFP-Pgc1275-321 in wt and doa10Δ cells was analyzed as in Fig. 1A. The graph shows the average of four independent experiments; error bars represent the standard deviation."
NCBI gene IDs:
"doa10: 854781"
Figure legend: "(D) Localization of 3HA-GFP-Pgc1275-321 in doa10Δ cells. The ER was visualized by expression of Sec63-Cherry. Scale bar: 5 µm."
NCBI gene IDs:
"doa10: 854781"
Figure legend: "(A) The degradation of 3HA-Pgc1-GPAT160-216, containing GPAT4 hydrophobic hairpin (aa160-216) replacing the one of Pgc1 was analyzed in cells with the indicated genotype as in Fig. 1A. The graph shows the average of three independent experiments; error bars represent the standard deviation."
NCBI gene IDs:
"GPAT4: 37852"
Figure legend: "(B) Localization of GFP-Pgc1-GPAT4160-216 in cells with the indicated genotype. Arrowheads indicate GFP-Pgc1-GPAT4160-216 labeling at the ER, marked with Sec63-Cherry. LDs were visualized upon staining with MDH. Scale bar: 5 µm."
NCBI gene IDs:
"GPAT4: 37852"
Figure legend: "(D) Localization of GFP-Pgc1-GPAT160-216 in are1Δ are2Δ lro1Δ dga1Δ doa10Δ mutant (no LDs doa10Δ). The ER was visualized by expression of Sec63-Cherry. Scale bar: 5 µm."
NCBI gene IDs:
"are1: 850415
are2: 855753
dga1: 854419
lro1: 855742
doa10: 854781"
Figure legend: "D Bar graphs showing the percentage of unique insertions in Nfib and Foxp1 in tumours, LM1, LM8, LM9, and other LM cell lines normalized to the total number of insertions in a given sample. Dots represent individual samples (Tumours n = 16, LM1/8/9 n = 9, Other LM n = 9), means ± s.d., two-tailed Mann-Whitney U-test, n.s. = not significant."
NCBI gene IDs:
"Foxp1: 108655
Nfib: 18028"
Figure legend: " E Bar graphs representing mean Nfib mRNA expression normalized to HR1 cells. n = 2 biological replicates and n = 2 technical replicates, means ± s.d., two-tailed Student's t-test, FC= fold change. F Bar graphs representing mean Foxp1 mRNA expression normalized to HR1 cells. n = 2 biological replicates and n = 2 technical replicates, means ± s.d., two-tailed Student's t-test, FC= fold change. "
NCBI gene IDs:
"Foxp1: 108655
Nfib: 18028"
Figure legend: "A The kinetics of LM1 (n = 10), LM9 (n = 4), LM1 Nfib KO (n = 4), and LM9 Nfib KO (n = 4) tumour growth upon orthotopic injection of 250 x 103 cells into NOD/SCID mice. Curves show means of tumour volume ± s.d., two-way ANOVA group analysis of the times to reach 250 mm3 (dashed line)."
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " B Kaplan-Meier plot depicting metastatic onset after tumour removal in mice injected orthotopically with LM1 (n = 5), LM9 (n = 4), LM1 Nfib KO (n = 3), or LM9 Nfib KO (n = 4) cells, two-tailed log-rank test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " C Nfib knockout abrogates metastasis in the orthotopic LM1 model. Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases at 25 (LM1) and 40 (KO) days after primary tumour removal; n = 3 mice, means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " D Nfib knockout impairs experimental metastases formation in the LM models. Kaplan-Meier plot showing metastatic incidence of animals inoculated i.v. with LM1 (n = 4), LM9 (n = 4), LM1 Nfib KO (n = 3), or LM9 Nfib KO (n = 5) cells, two-tailed log-rank test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " E Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases 16 days after i.v. injection of LM1 or LM1 Nfib KO cells, n = 3 mice, means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " A Kaplan-Meier plot depicting metastasis onset after tumour removal in mice injected orthotopically with HR1 gCtrl (n = 13) or HR1 g4Nfib (n = 14), two-tailed log-rank test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " B Nfib overexpression in HR1 parental cells enhances metastasis in the orthotopic model. Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases at day 20 (HR1 gCtrl) and day 2 (HR1 g4Nfib) after primary tumour removal. n = 4 mice, means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " C Nfib overexpression in the HR1 parental cells enhances experimental metastases. Kaplan-Meier showing metastatic incidence of animals inoculated i.v. with HR1 gCtrl (n = 11) or HR1 g4Nfib (n = 11) cells, two-tailed log-rank test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " D Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases 16 days after i.v. injection of HR1 gCtrl or HR1 g4Nfib cells. n = 4, means ± s.d., two tailed Student's t-test. "
NCBI gene IDs:
"Nfib: 18028"
Figure legend: " E NFIB overexpression enhances experimental metastases formation in the SUM159PT model. Kaplan-Meier survival analysis of animals inoculated i.v. with SUM159PT gCTRL (n = 5) or SUM159PT gNFIB (n = 6) cells, two-tailed log-rank test. "
NCBI gene IDs:
"NFIB: 4781"
Figure legend: " F Representative bioluminescence images (left panel) and bar plot quantification (right panel) of lung metastases 20 days after i.v. injection of SUM159PT gCTRL or SUM159PT gNFIB (n = 5), means ± s.d., two-tailed Student's t-test. "
NCBI gene IDs:
"NFIB: 18028"
Figure legend: " C ChIP was performed in all murine cell lines (Nfib high-expression and the respective Nfib low-expression or KO controls) against Nfib followed by qPCR with primers specific for Ero1l promoter region proximal to transcription starting site (Ero1l_492 and Ero1l_493). Meg3 was used as control. Graph shows qPCR results with % input method as indicated (Nfib high-expression vs. the respective Nfib low-expression or KO controls). n = 2 biological replicates and n = 2 technical replicates, means ± s.d., two-tailed Student's t-test, n.s. = not significant."
NCBI gene IDs:
"Ero1l: 50527
Meg3: 17263
Nfib: 18028"