EMBO/SourceData huggingface dataset:
Figure legend: "E Measurements of Pst DC3000 (AvrRpm1) and Pst DC3000 (AvrRpt2) growth. Data are shown as means ± SD (n = 3). Data information: Different letters indicate significant differences (Tukey's HSD test; P &lt; 0.05). Experiments were repeated 3 times with similar results. AvrRpm1, Pst DC3000 (AvrRpm1); AvrRpt2, Pst DC3000 (AvrRpt2); dpi, days post-inoculation."
NCBI gene IDs:
"AvrRpt2: 
AvrRpm1: 877422"
Figure legend: "F Colonization patterns of GFP-Pst DC3000 (AvrRpt2) in caspl mutant plants. IS, infected site; UIS, uninfected site. White dash lines indicate the boundary between IS and UIS. Scale bars, 100 μm."
NCBI gene IDs:
"AvrRpt2: 
caspl: 827238"
Figure legend: "A Visualization of CASPL4D1 proteins and lignin by mCherry and CADMAC fluorescence. Data information: pCASPL4D1::CASPL4D1-mCherry plants were pretreated with CADMAC and then inoculated with Pst DC3000 (AvrRpm1). mCh, mCherry; Chl, chlorophyll; hpi, hours post-inoculation. Scale bars, 20 μm (A)"
NCBI gene IDs:
"mCherry: 
CASPL4D1: 818538
AvrRpm1: 877422"
Figure legend: "B Magnified views of CASPL4D1 and lignin localization. Images in boxes were enlarged in the 2nd to 4th columns. Arrows indicate lignin deposition between cells. Data information: pCASPL4D1::CASPL4D1-mCherry plants were pretreated with CADMAC and then inoculated with Pst DC3000 (AvrRpm1). mCh, mCherry; Chl, chlorophyll; hpi, hours post-inoculation. Scale bars 10 μm (B)."
NCBI gene IDs:
"mCh: 
mCherry: 
CASPL4D1: 818538
AvrRpm1: 877422"
Figure legend: "C Relative fluorescence intensity profiles of CASPL1D1 (mCherry) and lignin (CADMAC) across transects indicated by dotted lines Data information: pCASPL4D1::CASPL4D1-mCherry plants were pretreated with CADMAC and then inoculated with Pst DC3000 (AvrRpm1)."
NCBI gene IDs:
"mCherry: 
CASPL4D1: 818538
AvrRpm1: 877422"
Figure legend: "D) Bar chart showing relative DsRed expression  of sensors containing different mutated target sequence of miR-17 Additionally, a sensor containing no target (n.t.) and two wildtype (WT) T17 target sites with 1x and 4x repeats were tested. Two-tailed unpaired t-tests were performed to compare H1  and  HES-2  for each sensor. *P-values &lt; 0.01, were considered very significant (**), &lt; 0.02 significant (*) and &gt; 0.5 not significant.. Representative scatterplots of HES-2 are shown on top. Data information: Each bar in D corresponds to mean +/- s.d. from at least three biological replicates."
NCBI gene IDs:
"miR-17: 406952"
Figure legend: "C) Combinatorial screen. Computational combinatorial screening  of Act1MAX and Act2MAX using the model depicted in A (left). Experimental  combinatorial screening in H1  (middle) with circuit schematic on top. Two different Act1-Act2  circuit configurations are depicted: PIT2-tTA (left) and rtTA-PIT2 (right). Calculated FF4  and Output levels are shown for On, Off and Off/On (FF4) or On/Off (Output). Color plots show normalized mCitrine   (left) and mCherry   (right) expression  levels for each Act1-Act2  combination. Color bars show lowest and highest expression  range on a linear scale with median as the midpoint D) Correlation plots with calculated values on X axis (Model) and experimental data on Y axis (Data). A linear regression model (y = a + b*x) was used to compare experimental data with model predictions. Linear fit and coefficient of determination (R2) as indicated. PIT2-tTA circuit (black), rtTA-PIT2 circuit (red)."
NCBI gene IDs:
"FF4: 
mCitrine: 
Act2: 6351
Act1: 10758"
Figure legend: "C) Bar chart showing the performance of circuit 1 - 3 in H1, HES-2 and HEK293. mCitrine   (top) and mCherry   (bottom) expression  was normalized to internal Ef1a-iRFP  control (rel.U.) (left) and further normalized to maximal mCitrine   (circuit 2) (top) or maximal mCherry   (bottom) (rel.U./Ctrl) (right). Samples below detection limit are labelled with b.d. Data information: Each bar show mean ± s.d. of at least three biological replicates."
NCBI gene IDs:
"iRFP: 
mCitrine: 
Ef1a: 1915"
Figure legend: "D) Bar chart showing performance of circuit 1 in response to administration of miRNA mimics in H1  (left) and HEK293  (right). First sample (with profile + + - - - for H1  and - - - - - for HEK292) has no miRNA administered. For the other samples, miRNA dose with highest change in response has been selected from miRNA titration experiments Maximal mCitrine  and mCherry  levels (as calculated in B) are depicted as yellow and red dashed lines, respectively. Chart is shown in a base 10 logarithmic scale. Data information: Each bar show mean ± s.d. of at least three biological replicates."
NCBI gene IDs:
"mCitrine: "
Figure legend: "E) Bar chart showing fine tuning of output using miSFITs library. Shown are FF4  / mCitrine  (top) and Output / mCherry  (bottom) of circuit 1 and 3 for different T17 variants. Data were normalized to control circuits. Data information: Each bar show mean ± s.d. of at least three biological replicates."
NCBI gene IDs:
"FF4: 
mCitrine: "
Figure legend: "A) Circuit schematics (left). Chart shows normalized Output production in response to changing amounts of plasmids encoding Act2, tTA (left) and PIT2 (right). Two transactivator constructs have been tested, onecontains miR302a/b target sites (Off), the other by mock  miRFF3/FF6  target sites (ON). Data information: Charts show mean ± s.d. of at least three biological replicates."
NCBI gene IDs:
"FF6: 
Act2: 6351
miR302a: 407028
miRFF3: 7033"
Figure legend: "Two-photon representative images of wild-type and Nlrp3-/- mice (5 and 15 months old). Scale bar: 20µm. Quantification of morphological parameters for wild-type and Nlpr3-/- mice after LPS injection"
NCBI gene IDs:
"Nlrp3: 216799
Nlpr3: 216799"
Figure legend: "Representative cortical images of MXO4 staining for APP and APP/Nlrp3-/- 15 months old mice. Scale bar: 50µm."
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Cortical amyloid plaque number and size quantification, and Amyloid-beta1-40 and 1-42 ELISA quantification for APP and APP/Nlrp3-/- mice (5 and 15 month old)"
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Two-photon images of microglia (e-GFP) cells clustering around amyloid plaque for APP and APP/Nlrp3-/- (5 and 15 months old). Scale bar: 20µm b) Quantification of morphological parameters in (a) (mean of 5-6±SEM)."
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Flow cytometry plots from APP and APP/Nlrp3-/- mice (15 months old), cells were gated on CD11b and MXO4 after microglia isolation. d) Relative Aβ microglia uptake quantification"
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Two-photon microglia (e-GFP) images from APP and APP/Nlrp3-/- in areas free of plaque (5 and 15 months old mice). Scale bar: 20µm Quantification of APP and APP/Nlrp3-/- morphological parameters for microglia in areas free of plaque"
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Iba-1 (green), CD169 (red) and MXO4 staining in plaque-associated areas (cortex and hippocampus) of 5 and 15 months old of APP and APP/Nlrp3-/-. Note the colocalization between Iba-1 and CD169 (white arrows) in APP 15-month-old mice 2 days post LPS injection. Scale bar: 20µm"
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "Iba-1, Ki67 and DAPI staining in the cortex and hippocampus of wild-type and Nlrp3-/- (5 and 15 months old). Microglia proliferates upon LPS injection (white arrows). Non-microglia cells proliferation was observed as well (yellow arrows) Scale bar: 20µm. Quantification of microglial proliferation in cortex (left panel) and hippocampus (right panel) (mean of 5±SEM; two-way ANOVA followed by Tukey's post hoc test, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001)."
NCBI gene IDs:
"Nlrp3: 216799"
Figure legend: "Iba-1, Ki67 and MXO4 staining in the cortex and hippocampus of APP and APP/Nlrp3-/- (5 and 15 months old). Microglia proliferates upon LPS injection (white arrows). Non-microglia cells proliferation was observed as well (yellow arrows). Scale bar: 20µm Quantification of microglial proliferation in cortex (left panel) and hippocampus (right panel)"
NCBI gene IDs:
"APP: 351
Nlrp3: 216799"
Figure legend: "A Representative images of GFP-gephyrin and GFP-G375D, expressed in hippocampal neurons at 9 + 4 days in vitro. Inset shows higher magnification. Scale bar = 20 µm.B Relative distribution of cluster density of gephyrin variants on dendrites from transfected neurons. Number of gephyrin clusters in each dendrite is normalized to 20 µm segments."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "C Representative segments of dendrites expressing GFP-gephyrin or GFP-G375D and immunostained with the presynaptic marker VGAT. Arrowheads show co-localization of gephyrin and VGAT clusters. Scale bar = 10 µm.D Quantification of co-localization between gephyrin and VGAT clusters (gephyrin-VGAT 77.4±2.9 %, G375D-VGAT 30±4.4 %). 31 GFP-gephyrin and 30 GFP-G375D neurons from three independent cultures were used for quantifications. Results are expressed as mean ± SEM"
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "A Representative dendritic segment of a neuron expressing GFP-G375D and 3B11-immunostained endogenous gephyrin (red).B Quantification of density and (C) size of endogenous gephyrin clusters in neurons expressing GFP, GFP-G375D or non-transfected neighboring neurons. 20 GFP-G375 and non-transfected neurons and 15 GFP-expressing neurons from three independent cultures were used for quantifications. Results are expressed as mean ± SEM and data were analyzed by t-test."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "A Representative neurons expressing myc-tagged gephyrin or G375D and pHluorin-tagged GABAAR γ2. Surface-expressed γ2-subunits were immunolabelled with GFP-antibodies in red, and the pHluorin-fluorescence (total γ2 levels) are shown in green.B Size and C fluorescence (fl.) intensity of surface-expressed γ2 clusters in myc-gephyrin and myc-G375D-expressing dendrites (n = 20 myc-gephyrin and 21 myc-G375D neurons in B and 18 myc-gephyrin and myc-G375D neurons in C)D Mean γ2-pHluorin fluorescence in myc-gephyrin and myc-G375D-expressing dendrites (n = 20 and 18 myc-gephyrin and myc-G375D expressing neurons).E Number of surface γ2 clusters on dendrites normalized to 20 µm segments (n = 18 myc-gephyrin and myc-G375D expressing neurons). At least three independent cultures were used for each experiment. Results are expressed as mean ± SEM; Data were analyzed by t-test."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "F Representative traces of miniature recordings on cultured hippocampal neurons transfected either with GFP-gephyrin or GFP-G375D.G mIPSC amplitudes from neurons expressing GFP-gephyrin or GFP-G375D. Numbers show analyzed cell-numbers. Data were analyzed by one-way ANOVA and post-hoc Tukey test.H Frequency of the mIPSCs from neurons expressing GFP-gephyrin or GFP-G375D. The horizontal line within the boxes indicates the median frequency for the represented condition (bottom/top boundary indicates the 25th /75th percentile). Top and bottom error bars indicate the 90th and 10th percentiles, respectively. Analysis by Kruskal-Wallis test followed by post-hoc Mann-Whitney test."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "A Western blot of HEK293 cells expressing GFP-tagged gephyrin or G375D using GFP-specific and polyclonal gephyrin antibodies. Note that both gephyrin variants are stably expressed and show no degradation in the cells. Asterisk in the poly-gephyrin blot shows endogenous gephyrin."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "B SDS-PAGE of E. coli expressed and purified 6His-gephyrins and CD spectra. Mean residue ellipticity of gephyrin and G375D show profiles with minima at 208 nm and 222 nm."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "C Size-exclusion chromatography of purified 6His-gephyrin and isolated E-domain variants. Full-length gephyrins and isolated E-domains were loaded onto a Superose 6 gel filtration column and elution of the protein was monitored by measuring absorbance at 280 nm (mAU, milliabsorbance units)."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "D Co-immunoprecipitation of HEK293 cell lysates expressing myc-tagged gephyrin together with GFP-tagged gephyrin or G375D using myc-specific antibodies. Control IgG were used to show specificity of the assay."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "B Representative pulldown-experiment and quantification of 6His-tagged gephyrin and G375D using immobilized α3-peptide. 6His-tagged gephyrin variants were expressed in E. coli cells, purified to homogeneity and incubated with α3-peptides immobilized to NeutrAvidin beads. n = 3, normalized to 6His-gephyrin. 38.9±1.5% G375D were pulled down."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "C Representative SPR sensograms of 6His-gephyrin and 6His-G375D binding to immobilized GABAAR α3-peptide. Response units are plotted against time. Injected gephyrin concentrations were 0.5, 2, 10, 25, 40 µM."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "D SPR binding isotherms of a representative experiment as in C. Calculated KD values of 3.2 and 10 µM for gephyrin and G375D, respectively, are indicated by vertical lines. The experiment was repeated three times with two independently purified protein batches and two sensor chips."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "E Representative ITC binding isotherms of the cytoplasmic loop of the GlyR ß-subunit (GlyR-ßL, residue 378-426) and 6His-tagged gephyrin or G375D. The GlyR-ßL-gephyrin binding data (black) were fitted in a two-side model, whereas G375D binding could only be fitted to a single binding site. Dissociation constants KD and stoichiometry (N = number of binding sites) are given for each binding component. n = 5 from two independently purified protein batches. Results are expressed as mean ± SEM."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "A Biosynthesis pathway of Moco with all stable intermediates. Gephyrin G- and E-domain catalyze the last two steps as depicted.B In vitro Moco-synthesis assay using 150 pmol purified 6His-tagged gephyrins. D580A is an activity-deficient gephyrin mutation previously identified in Moco-deficient patients (Reiss et al., 2011). Assays without the addition of gephyrin (-gephyrin) or molybdenum (-Mo) served as internal controls of the assay (see also (Belaidi & Schwarz, 2013)).C Experiment as in B but with pre-incubation of 6His-gephyrin/G375D in a 1:1 ratio to simulate the heterozygous mutation. n = at least 3 with two independently purified protein batches. Results are expressed as mean ± SEM."
NCBI gene IDs:
"gephyrin: 10243"
Figure legend: "IIA1.6 B lymphoma cells were transfected to transiently express centrin1-GFP (red) and EB3-mCherry (green) and video-recorded at the contact site with the glass coverslip (left) and at the centrosome (right). Scale bar: 3 µm."
NCBI gene IDs:
"GFP: 
mCherry: 
centrin1: 1068
EB3: 22924"
Figure legend: "IIA1.6 B lymphoma cells were transfected to transiently express centrin1-VCA-GFP (bottom) or centrin1-GFP (top) as control prior to be fixed and stained for α-tubulin (left column) and F-actin (middle column). The GFP signal of centrin1 or centrin1-VCA is shown in the right column to illustrate the proper centrosome targeting. Scale bar: 3 µm."
NCBI gene IDs:
"GFP: 
centrin1: 1068"
Figure legend: "Histograms show the quantifications of the amount of polymerized fluorescent tubulin (right) and filamentous actin at the centrosome (left). Values correspond to the fraction of fluorescence in a 2-micron-wide area around the centrosome relative to the total fluorescence in the cell. Measurements were pooled from 3 independent experiments; centrin1-GFP: n= 88, centrin1-VCA-GFP: n= 87. Errors bars represent standard deviations. P values were calculated with Mann-Whitney test."
NCBI gene IDs:
"GFP: 
centrin1: 1068"
Figure legend: "Percentage differences of F-actin and microtubule fluorescence intensities at the centrosome were compared in cells transfected either with centrin1-VCA-GFP or with centrin1-GFP. Errors bars represent standard deviations. P values were calculated with one-sample t-test (i.e. comparison to a theoretical mean of \"0\")."
NCBI gene IDs:
"GFP: 
GFP.: 
centrin1: 1068"
Figure legend: "The graph shows the variations of the total amount of tubulin per cell with respect to the content of actin at the centrosome. The two lines correspond to linear regressions of the two sets of data relative to cells transfected with centrin1-VCA-GFP or centrin1-GFP."
NCBI gene IDs:
"GFP: 
centrin1: 1068"
Figure legend: "Two sets of representative images showing fluorescent microtubules and actin filaments assembled from isolated centrosomes. Centrosomes were isolated from Jurkat cells expressing centrin1-GFP. Upper and lower lines show actin filaments and microtubules radiating from two distinct centrosomes with low (top) and high (bottom) densities of actin filaments. Scale bars: 10 μm."
NCBI gene IDs:
"GFP.: 
centrin1: 1068"
Figure legend: "RPE1 cells stably expressing centrin1-GFP were plated for 3 h on coverslips coated with different ratios (100:0; 50:50 or 1:99) of fibronectin and PLL-PEG prior to fixation and staining for F-actin (top line and magnified views around centrosome below. Scale bars: 10 µm and 2 µm, respectively) and α-tubulin (bottom line. Scale bar: 10 µm)."
NCBI gene IDs:
"GFP: 
centrin1: 1068"
Figure legend: "B) Binding affinity  of CodB  for cytosine  as measured using the thermostability assay. Cytosine  was titrated into detergent solubilised membranes   from cells overexpressing CodB. The  Kd  was estimated to be 51 ± 9 µM. The measurements are the average of 4 independent titrations with error bars of the s.e.m."
NCBI gene IDs:
"CodB: 57310904"
Figure legend: "Western blot of CHIR-treated Hoxb8-FL WT and Bax-/-Bak-/- cells using the indicated antibodies."
NCBI gene IDs:
"Bak: 12018
Bax: 12028"
Figure legend: "WT and Bax-/-Bak-/- Hoxb8-FL cells were treated for up to 8h with 500 nM CHIR and p21 mRNA levels were compared to Hprt in response to CHK1-inhibition by real-time RT-qPCR. Bars represent means ± S.D. of independent experiments (n=4-6), performed in duplicates."
NCBI gene IDs:
"Hprt: 
Bak: 12018
Bax: 12028
p21: 12575"
Figure legend: "CD34+ cells were transduced with a BCL2-encoding lentivirus or a control virus, along with IRES-GFP with a MOI of 10 for two consecutive days, followed by CHK1i application for 48 h. Survival was assessed using Nicoletti staining and flow cytometry."
NCBI gene IDs:
"GFP: 
BCL2: 596"
Figure legend: "Colony formation potential of human CD34+ HSPCs transduced with empty control or BCL2 encoding virus. Colonies were counted 10 days post seeding of 1000 CD34+ cells per plate/sample. \"Myeloid colonies\" include granulocyte/monocyte, monocyte, granulocyte and GEMM (granulocyte, erythroid, monocyte/macrophage and megakaryocyte) colonies."
NCBI gene IDs:
"BCL2: 596"
Figure legend: "Representative pictures of E13.5 and E18.5 embryos of the indicated genotypes. Scale bar E13.5 = 1 mm, E18.5 = 5 mm. Quantification of fetal liver cell number at E13.5. Bars represent means ± S.E.M. (N=4 for Chk1fl/+, N=3 for Chk1fl/+ Vav-Cre and N=4 for Chk1fl/- Vav-Cre)."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Representative H&amp;E-staining of fetal liver sections from E13.5 embryos. Black arrows indicate lack of erythroid cells in the blood vessels of Chk1fl/- Vav-Cre fetal livers. Scale bar = 500 µM."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Single cell suspensions of fetal livers from Chk1fl/+, Chk1fl/+ Vav-Cre and Chk1fl/- Vav-Cre embryos were stained with antibodies recognizing ckit, CD71, Ter119 or CD45 to assess primitive and definitive erythropoiesis by flow cytometry. Large 'EryA' erythroblasts (CD71high Ter119high FSChigh), smaller, more mature 'EryB' erythroblasts (CD71high Ter119high FSClow); most mature erythroblast subset is EryC (CD71low Ter119high FSClow). Bars represent means ± S.E.M. ctrl N=5 (pooled N=4 Chk1fl/+ and N=1 Chk1fl/+ Vav-Cre), N=3 for Chk1fl/- Vav-Cre."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Representative dot-plots of the LSK cell phenotype of Chk1fl/+ and Chk1fl/- Vav-Cre embryos observed in E13.5 fetal livers and relative distribution / absolute numbers of LKhi and LSKhi cells, as well as LKlow and LSKlow cells, found in the fetal liver on E13.5. Bars represent means ± S.E.M. N=4 for Chk1fl/+ and N=4 for Chk1fl/- Vav-Cre."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Colony formation potential of FACS-sorted LSK cells (left panel, N=4 for Chk1fl/+ and N=5 for Chk1fl/- Vav-Cre) and total fetal liver cells (right panel, N=3 for Chk1fl/+ and N=6 for Chk1fl/- Vav-Cre) in methyl-cellulose assays. Bars represent means ± S.E.M."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Flow cytometric analysis of LSKhi or LSKlow cells stained with antibodies specific for CD34 and Flt3 (N=4 for Chk1fl/+ and N=4 for Chk1fl/- Vav-Cre), to discriminate LT- and ST-HSC from MPP within the LSK-subset. Bars represent means ± S.E.M."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of fetal liver cryosections of the indicated genotypes at E13.5 and quantification. Scale bar 100x = 200 µM, 400x = 50 µM. The percentage of TUNEL+ area/field was assessed across three random fields from two control (Chk1fl/+) and two knock-out embryos (Chk1fl/- Vav-Cre) using ImageJ, as reported by us before (60). Bars represent means ± S.D."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Total fetal liver cell suspensions of Chk1fl/+ (+/+), Chk1fl/+ Vav-Cre (+/-) and Chk1fl/- Vav-Cre (-/-) E13.5 embryos were processed for Western blot analysis using the indicated antibodies. Each lane represents samples isolated from an individual littermate embryo."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Total E13.5 fetal liver cells were processed for intracellular Ki67 staining in combination with cell surface antibody staining to discriminate LK (top panel) from LSK (lower panel) cells. Shown here are representative dot-plots of Ki67 and DAPI staining in LK and LSK cells, quantified in (E). Bars represent means ± S.E.M. N=4 for Chk1fl/+ and N=5 for Chk1fl/- Vav-Cre."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477"
Figure legend: "Representative dot-plots and quantification of E13.5 fetal liver LSKhi/LSKlow cell phenotypes observed in embryos of the indicated genotypes. N=21 for Chk1fl/+, N=5 for Chk1fl/- Vav-Cre, N=16 for Chk1fl/+ Vav-BCL2, N=5 for Chk1fl/- Vav-Cre Vav-BCL2, N=2 for p53-/- Chk1fl/+ and N=4 for p53-/- Chk1fl/- Vav-Cre."
NCBI gene IDs:
"Chk1: 12649
Cre: 2777477
p53: 22059"
Figure legend: "Flow cytometry-based analysis of LSK cells stained with antibodies specific for CD34 and Flt3, to discriminate LT- and ST-HSC from MPP within the LSK subset in fetal livers of embryos of the indicated genotypes. Left: N=25 for Chk1fl/+, N=7 for Chk1fl/+ Vav-Cre and N=7 for Chk1fl/- Vav-Cre. Right: N=16 for Chk1fl/+ Vav-BCL2, N=6 for Chk1fl/+ Vav-Cre Vav-BCL2 and N=5 for Chk1fl/- Vav-Cre Vav-BCL2."
NCBI gene IDs:
"BCL2: 596
Chk1: 12649
Cre: 2777477"
Figure legend: "Analysis of the colony formation potential of E14.5 total fetal liver cells of the indicated genotypes in methylcellulose. N=3 for controls (pooled; 1 Chk1fl/+, 2 Chk1fl/+ Vav-Cre), N=3 for Chk1fl/- Vav-Cre and N=3 for Chk1fl/- Vav-Cre Vav-BCL2."
NCBI gene IDs:
"BCL2: 596
Chk1: 12649
Cre: 2777477"
Figure legend: "Three independent Chk1fl/fl mT/mG (coloured) and mT/mG (grey) Hoxb8-FL progenitor cell lines were transduced with a retroviral construct carrying Cre (pMIP-CRE-IRES-PURO) and monitored over time for GFP-expression. Shown here are three technical replicates per genotype. Additionally, Chk1fl/fl mT/mG Hoxb8-FL progenitor cells were FACS-sorted for GFP+ and dsRED+ cells 48h after transduction with the Cre-construct. Sorted cells were processed for western blotting using the indicated antibodies."
NCBI gene IDs:
"mG: 
mT: 
Chk1: 12649
Cre: 2777477
CRE: 2777477
pMIP: 17339"