EMBO/SourceData huggingface dataset:
Figure legend: "(E−H) Induction of GOMED by the deletion of Golgi PI(4) kinase. (F) Representative electron microscopy image of pik1-83/atg5Δpep4Δ cells at 3 hr after temperature shift. AP-like structure (arrow) and AB-like structures were generated. Scale bar = 0.2 µm. (G) The number of AP-like and AB-like structures was counted using EM images. Error bars indicate s.e.m. (atg5Δpep4Δ cells at 37 °C: n = 54 and pik1-83/atg5Δpep4Δ cells at 37 °C: n = 63). *p &lt; 0.01 vs the value of atg5Δpep4Δ cells."
NCBI gene IDs:
"atg5: 855954
pep4: 855949
pik1: 855454"
Figure legend: "(E−H) Induction of GOMED by the deletion of Golgi PI(4) kinase. (H) Sec7-HA-expressing pik1-83/atg5Δ cells were incubated at 37 °C (temperature shift) for 3 hr, and freeze replica immunolabelling was performed as described in Methods. Sec7-HA-positive signals (labelled with 15 nm gold particle) were observed on the surface membrane of AP-like structure and Golgi membrane. Scale bar = 0.1 µm."
NCBI gene IDs:
"atg5: 855954
pik1: 855454"
Figure legend: "(I−L) Genetic (I, J) and pharmacological (K, L) alterations in anterograde trafficking from the Golgi are required for GOMED. (I) Indicated cells were incubated at 37 °C (temperature switch) for 3 hr, and the cells containing AB-like structures were counted by phase-contrast microscopy. *p &lt; 0.01 vs the value of atg5Δpep4Δ cells."
NCBI gene IDs:
"atg5: 855954
pep4: 855949"
Figure legend: "Genetic (I, J) and pharmacological (K, L) alterations in anterograde trafficking from the Golgi are required for GOMED. (K, L) atg5Δpep4Δ cells were treated with (CBM) or without (NT) CBM (1 mM, 3 hr), and cells containing AB-like structures were counted by phase-contrast microscopy (K). * p &lt; 0.01 vs the value of NT."
NCBI gene IDs:
"atg5: 855954
pep4: 855949"
Figure legend: "Genetic (I, J) and pharmacological (K, L) alterations in anterograde trafficking from the Golgi are required for GOMED. (K, L) atg5Δpep4Δ cells were treated with (CBM) or without (NT) CBM (1 mM, 3 hr). Cells were also observed using EM (L). AB-like structures were observed. Scale bar = 0.5 µm."
NCBI gene IDs:
"atg5: 855954
pep4: 855949"
Figure legend: "(A−I) Induction of GOMED in Atg5 KO MEFs by CBM treatment. (A, B) EM analysis revealed the formation of AP-like and AL-like structures. Atg5 KO MEFs were treated with CBM (2 mM) for 3 hr. A representative low-magnification image (A) and high-magnification images (B) are shown. Scale bar = 1 µm (A) and 0.2 µm (B). The arrowheads and arrows indicate AL-like and AP-like structures, respectively."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(A−I) Induction of GOMED in Atg5 KO MEFs by CBM treatment. (C-F) Involvement of Golgi membranes in the generation of AL-like structures. (C) CLEM analysis of mCherry-syntaxin 6-expressing Atg5 KO MEFs. Cells were treated with CBM (2 mM) for 24 hr and observed using fluorescence and electron microscopy. mCherry-syntaxin 6puncta merged with the AL-like structures. Scale bar = 5 µm. \"N\" indicates nucleus. A magnified image of the dashed square is shown in the inset. Scale bars = 2 µm."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(A−I) Induction of GOMED in Atg5 KO MEFs by CBM treatment. (C-F) Involvement of Golgi membranes in the generation of AL-like structures. (D, E) Requirement of Grasp65 in the generation of AL-like structures. Atg5 KO and Grasp65-silenced Atg5 KO MEFs were treated without (NT) or with CBM (2 mM) for the indicated times followed by immunostaining with an anti-Lamp2 antibody. Representative images (at 24 h) are shown in (D). Scale bars = 5 µm. A magnified image of the dashed square is shown in the inset. Scale bars = 1 µm. CBM induced large ring-like Lamp2 fluorescence. (E) Percentages of cells with large ring-like Lamp2 immunofluorescence (mean ± s.e.m., n = 4). *p &lt; 0.01 vs the value of Atg5 KO MEF."
NCBI gene IDs:
"Atg5: 11793
Grasp65: 74498"
Figure legend: "(A−I) Induction of GOMED in Atg5 KO MEFs by CBM treatment. (C-F) Involvement of Golgi membranes in the generation of AL-like structures. (F) Colocalization of Lamp2 and GFP-syntaxin 6 in CBM-treated Atg5 KO MEFs. GFP-syntaxin 6-expressing Atg5 KO MEFs were treated with (CBM) or without (NT) CBM (2 mM) for 6 hr and immunostained with an anti-Lamp2 antibody. The ring-like Lamp2 fluorescence merged with the signal for syntaxin 6. Scale bar = 5 µm. A magnified image of the dashed square is shown in the inset. Scale bars = 2 µm."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(G) The monomeric red fluorescent protein (mRFP)-green fluorescent protein (GFP) tandem protein assay revealed the induction of GOMED. Atg5 KO MEFs stably expressing tandem mRFP-GFP were incubated without (no treatment) or with CBM (2 mM) for 24 hr. Red signals indicate acidic compartments. Lysosomes were counterstained with an anti-Lamp2 antibody (cyan). Scale bars = 5 µm. Regions of interest (ROI) are indicated by dashed squares. Arrowheads indicate GOMED structure. Scale bars = 2 µm."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(A−I) Induction of GOMED in Atg5 KO MEFs by CBM treatment. (H, I) Degradation of GFP-fused proteins indicated the induction of GOMED. Atg5 KO MEFs stably expressing VSVG-GFP (H) or M6PR-GFP (I) were incubated with CBM (5 mM) for the indicated times. Cell lysates were subjected to immunoblotting with the anti-GFP antibody."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(J−M) Genetic induction of GOMED in Atg5 KO MEFs. Atg5 KO MEFs were treated with the indicated small interfering (si)RNAs for 24 hr and GOMED was assessed by EM (J-L). Representative images are shown in (J, K). Arrows indicate AL-like structures. Scale bars = 2 µm. (L) The number of AP-like and AL-like structures was calculated from the EM images of cells treated with the indicated siRNAs. Error bars indicate s.e.m. (siControl: n = 23, siPI4k2α + siPI4k3β: n = 20, siArfaptin1: n = 20). *p &lt; 0.01 vs the value of siControl."
NCBI gene IDs:
"PI4k3β: 
Arfaptin1: 99889
Atg5: 11793
PI4k2α: 84095"
Figure legend: "(J−M) Genetic induction of GOMED in Atg5 KO MEFs. Atg5 KO MEFs were treated with the indicated small interfering (si)RNAs for 24 hr and GOMED was assessed by the mRFP-GFP tandem protein assay (M). (M) The same experiments as in (G) were performed using cells treated with the indicated siRNAs. Representative images are shown. Arrowheads indicate GOMED structure. Scale bars = 5 µm."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(A) Suppression of insulin secretion in Atg5 knockout (KO) MIN6 cells after glucose deprivation. Atg5 KO MIN6 cells were incubated with normal culture medium (NT) or glucose-depleted medium for 1 hr. The amount of secreted insulin is expressed as the percentage of that of the untreated control (NT) (mean ± s.e.m, n = 3). *p &lt; 0.01 vs the value of NT."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(B, C) EM analysis revealed the induction of GOMED structures. Atg5 KO MIN6 cells were subjected to glucose deprivation for 1 hr. Representative images are shown. The white arrow and black arrows indicate the AP-like structure and insulinvesicles, respectively. Scale bars = 0.2 µm. (D) The number of AP-like and AL-like structures was calculated from the EM images of cells left untreated (NT) or subjected to glucose deprivation. Error bars indicate s.e.m. (NT: n = 17, glucose deprivation: n = 84). *p &lt; 0.01 and #p &lt; 0.05 vs the value of NT."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(E) Involvement of Golgi membrane in the generation of GOMED. Lamp1-GFP/mCherry-Syntaxin 6-expressing Atg5 KO MIN6 cells were subjected to glucose deprivation, and analysed by TEM and confocal microscopy. CLEM analysis revealed that AL-like structures (arrows) merged with Lamp1-GFP fluorescence and Syntaxin 6 fluorescence. Scale bars = 5 µm. A magnified image of the dashed square is shown in the inset. Scale bars = 2 µm."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(F) GOMED-mediated degradation of (pro)insulin by glucose deprivation of Atg5 KO MIN6 cells. Atg5 KO MIN6 cells were incubated in glucose-depleted medium in the absence or presence of bafilomycin A1 (10 nM) for the indicated times. The cell lysates were then subjected to immunoblot analysis using an anti-(pro)insulin antibody."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(G) Immunofluorescence analysis of (pro)insulin and Lamp2. Atg5 KO MIN6 cells were left untreated (NT) or subjected to glucose deprivation for 1 hr in the presence of E64d (25 µM) or bafilomycin A1 (10 nM), and were stained with anti-(pro)insulin and anti-Lamp2 (lysosomal marker) antibodies and observed by fluorescence microscopy. Scale bar = 1 µm. Arrowheads indicate the colocalization of (pro)insulin with GOMED structures."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(A−D) EM analysis demonstrated the induction of GOMED. Primary Atg7 KO β-cells were subjected to glucose deprivation for 1 hr. A representative AP-like structure containing insulingranule is shown in (A). An AL-like structure containing insulingranules is shown in (B). Scale bars = 0.2 µm."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(A−D) EM analysis demonstrated the induction of GOMED. Primary Atg7 KO β-cells were subjected to glucose deprivation for 1 hr. (C, D) GOMED structures containing Golgi vesicles. An AP-like structure containing multiple Golgi vesicles (arrows) (C) and an AL-like structure fusing with a lysosome (arrowhead) (D) are shown. Scale bar = 0.5 µm. A magnified image of the dashed rectangle is shown in the inset. Scale bar = 0.2 µm. AP-like structure contained double-membrane."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(E) The total number of AL-like structure was calculated from the EM images of WT and Atg7 KO β-cells after glucose deprivation. Error bars indicate s.e.m. (n = 46−163). *p &lt; 0.01 and #p &lt; 0.05 vs the value of 0 min."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(F) The total number of GOMED structure was calculated from the EM images of Atg7 KO β-cells after glucose deprivation in the absence or presence of bafilomycin A1 (10 nM). Error bars indicate s.e.m. (n = 109−116). *p &lt; 0.01 vs the value of 60 min."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(G−J) EM analysis showed the induction of mitophagy. WT and Atg7 KO β-cells were subjected to glucose deprivation for 1 hr. Representative images showing mitophagy in Atg7 KO β-cells are shown in (G). Arrowhead (G) indicates mitophagy. Scale bars = 0.5 µm. (H, J) The number of structures representative of mitophagy (H) per cell was calculated from the EM images of β-cells after glucose depletion. Error bars indicate s.e.m. (n = 46−163). *p &lt; 0.01 and #p &lt; 0.05 vs the value of 0 min."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(G−J) EM analysis showed the induction of crinophagy/SINGD. WT and Atg7 KO β-cells were subjected to glucose deprivation for 1 hr. Representative images showing crinophagy/SINGD in WT β-cells are shown in (I). White arrow (I) indicates crinophagy/SINGD. Arrows indicate insulingranules (I). Scale bars = 0.5 µm. (H, J) The number of structures representative of crinophagy/SINGD (J) per cell was calculated from the EM images of β-cells after glucose depletion. Error bars indicate s.e.m. (n = 46−163). *p &lt; 0.01 and #p &lt; 0.05 vs the value of 0 min."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(K) The number of (pro)insulingranule-containing AL-like structure was calculated from the EM images of wild-type (WT) and Atg7 KO β-cells after glucose deprivation. Error bars indicate s.e.m. (n = 46−163). *p &lt; 0.01 and #p &lt; 0.05 vs the value of 0 min."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "Overexpression of WT OGT, but not catalytic-inactive OGT mutant, increased the O-GlcNAc levels of TDP-43. OGT protein levels were examined by immunoblotting.   Quantification of (G).   "
NCBI gene IDs:
"OGT: 8473"
Figure legend: "SDS-soluble and insoluble fractions were isolated from SH-SY5Y cells expressing WT or catalytic-inactive mutant OGT treated with 20 μM EA, and the fractions were analyzed by immunoblotting. Quantification of (F)."
NCBI gene IDs:
"OGT: 8473"
Figure legend: "Cells expressing the indicated forms of GFP-TDP-43 were subjected to immunoprecipitation, and the O-GlcNAcylated levels of TDP-43 were examined.   Quantification of (G).   "
NCBI gene IDs:
"GFP: 
TDP-43: 23435"
Figure legend: "CFTR splicing assay was performed in cells with knockdown of either OGT, TDP-43, or combination. ACTB levels served as loading controls (top panel). The indicated protein levels were examined by immunoblotting (bottom panel)."
NCBI gene IDs:
"ACTB: 
CFTR: 12638
OGT: 108155
TDP-43: 230908"
Figure legend: "CFTR splicing assay was examined in siTDP-43 cells overexpressing the WT or mutant OGT by PCR analysis. ACTB levels served as loading controls (top panel). Various protein levels were examined by immunoblotting (bottom panel)."
NCBI gene IDs:
"ACTB: 
CFTR: 12638
OGT: 8473
TDP-43: 230908"
Figure legend: "Representative PCR analysis to examine expression levels of the spliced mRNA isoform containing exon 2a of STMN2 in SH-SY5Y cells with the indicated constructs (top panel). TDP-43 protein levels were examined by immunoblotting (bottom panel)."
NCBI gene IDs:
"STMN2: 11075"
Figure legend: "C Fraction of expressed genes (genes with non-zero counts in DESeq2) were calculated which exhibit individual or combinations of differential gene expression (DGE), differential transcript usage (DTU) and/or  alternative splicing   (AS) events in HEK293  and HeLa  WT cells overexpressing UPF3A  using the respective computational analysis (cutoffs are indicated). AS and DTU events were collapsed on the gene level. For DGE, p-values were calculated by DESeq2 using a two-sided Wald test and corrected for multiple testing using the Benjamini-Hochberg method. For DTU, p-values were calculated by IsoformSwitchAnalyzeR (ISAR) using a DEXSeq-based test and corrected for multiple testing using the Benjamini-Hochberg method. For AS, p-values were calculated by LeafCutter using an asymptotic Chi-squared distribution and corrected for multiple testing using the Benjamini-Hochberg method."
NCBI gene IDs:
"UPF3A: 65110"
Figure legend: "E Read coverage of SMG5 from WT HEK293  and HeLa RNA-seq data with or without induced UPF3A  overexpression shown as Integrative Genomics Viewer (IGV) snapshot. Differential gene expression (from DESeq2) is indicated as log2 fold change (log2FC) on the right. Schematic representation of the protein coding transcript below."
NCBI gene IDs:
"SMG5: 23381
UPF3A: 65110"
Figure legend: "A Western blot  analysis of WT and UPF3A KO cells (clones 4, 14 and 20) with the indicated siRNA treatments (n=1). UPF3A and UPF3B protein levels were detected, Tubulin  serves as control. The asterisk indicates unspecific bands."
NCBI gene IDs:
"UPF3A: 65110"
Figure legend: "B Quantitative RT-PCR of the indicated cell lines treated with the indicated siRNAs for 2 or 6 days. For RSRC2  and SRSF2  the ratio of NMD isoform to canonical isoform was calculated. ZFAS1 expression  was normalized to C1orf43 reference. Data points and means are plotted as log2 fold change (log2FC) (n=3 for RSRC2  and SRSF2, n=4 for ZFAS1)."
NCBI gene IDs:
"C1orf43: 25912
RSRC2: 65117
SRSF2: 6427
ZFAS1: 441951"
Figure legend: "D,E Volcano plots showing the differential gene expression analyses from the indicated  RNA-Seq datasets (D UPF3A  KO clone 14, E UPF3A  KO clone 20). The log2 fold change is plotted against the -log10 adjusted p-value (adj. p-value). P-values were calculated by DESeq2 using a two-sided Wald test and corrected for multiple testing using the Benjamini-Hochberg method"
NCBI gene IDs:
"UPF3A: 65110"
Figure legend: "A  Western blot  analysis of WT and UPF3B  KO cells (clones 90 and 91) combined with the indicated knockdowns (n=1). UPF3A  and UPF3B  (AK-141) protein levels were detected, Tubulin  serves as control. Protein levels of UPF3A  were quantified, normalized to tubulin  expression, and shown as datapoints and mean. Fold-changes of relevant conditions are shown. The asterisk indicates unspecific bands."
NCBI gene IDs:
"UPF3B: 65109"
Figure legend: "B Quantitative RT-PCR of the indicated cell lines with the indicated knockdowns. For RSRC2  and SRSF2  the ratio of NMD isoform to canonical isoform was calculated. Data points and means are plotted as log2 fold change (log2FC, n=3)."
NCBI gene IDs:
"RSRC2: 65117
SRSF2: 6427"
Figure legend: "A Western blot  analysis of WT, UPF3B  KO and UPF3A-UPF3B  double KO cells (clones 1 and 2) (n=1). UPF3A  and UPF3B protein levels were detected, Tubulin  serves as control. The asterisk indicates unspecific bands."
NCBI gene IDs:
"UPF3A: 65110
UPF3B: 65109"
Figure legend: "C Quantitative RT-PCR  of the indicated samples with the indicated KDs. For RSRC2  and SRSF2  the ratio of NMD isoform to canonical isoform was calculated. ZFAS1 expression  was normalized to C1orf43  reference. Data points and means are plotted as log2 fold change (log2FC, n=3)."
NCBI gene IDs:
"C1orf43: 25912
RSRC2: 65117
SRSF2: 6427
ZFAS1: 441951"
Figure legend: "E Northern blot  analysis of globin  reporter and xrFrag. Ethidium bromide stained 28S and 18S rRNAs are shown as controls. Quantification results are shown as data points and mean (n=3)."
NCBI gene IDs:
"18S rRNAs: 
28S: 
globin: 3043"
Figure legend: "A-C Volcano plots of label free mass spectrometry-based analysis of the interaction  partners of UPF2  in WT cells treated with control siRNAs and the UPF3B KO clone 90 and dKO clone 1 both treated with siRNAs targeting UPF3B  (n = 4 biologically independent samples). (A) FLAG-UPF2   in WT against FLAG-GST   control in WT cells, (B) UPF2   in 3B KO cells against FLAG   control in WT cells, (C) UPF2   in dKO cells against FLAG   control in WT cells. Points labeled in purple indicate NMD factors; points labeled in turquoise indicate EJC components. Cut offs: Log2 fold change ≥ 0"
NCBI gene IDs:
"FLAG: 
GST: 
UPF2: 26019
UPF3B: 65109"
Figure legend: "E Representative  Western blot  after FLAG co-immunoprecipitation (IP) of FLAG-tagged GST  (control) or UPF3B  WT and single mutant constructs in UPF3B  KO cells (n = 3)."
NCBI gene IDs:
"FLAG: 
GST: 
UPF3B: 65109"
Figure legend: "F Western blot  analysis of WT and UPF3B  KO clone 90 with Luciferase  and UPF3A  KDs respectively. Monitored expression of the FLAG-tagged UPF3B  rescue construct shown in (D). Rescue construct protein levels were detected with anti-FLAG and anti-UPF3B  (AK-141) antibodies. Tubulin serves as control (n=1)."
NCBI gene IDs:
"Luciferase: 
UPF3A: 65110
UPF3B: 65109"
Figure legend: "G  Quantitative RT-PCR of the samples from (F). For RSRC2 and SRSF2  the ratio of NMD isoform to canonical isoform was calculated. ZFAS1 expression  was normalized to C1orf43  reference. Data points and means are plotted as log2 fold change (log2FC, n=3)."
NCBI gene IDs:
"C1orf43: 25912
RSRC2: 65117
SRSF2: 6427
ZFAS1: 441951"
Figure legend: "B Western blot  analysis of WT and UPF3B  KO clone 90 with Luciferase  and UPF3A KDs respectively. Monitored expression of the FLAG-tagged  UPF3A and  UPF3B rescue construct shown in (A). Rescue construct protein levels were detected with anti-FLAG, anti-UPF3A  and anti-UPF3B  (AK-141) antibodies. Tubulin  serves as control (n=1). The asterisk indicates unspecific bands."
NCBI gene IDs:
"FLAG: 
Luciferase: 
UPF3A: 65110
UPF3B: 65109"
Figure legend: "C Quantitative RT-PCR of the samples from (B). For RSRC2  and SRSF2  the ratio of NMD isoform to canonical isoform was calculated. Data points and means are plotted as log2 fold change (log2FC) (n=3)."
NCBI gene IDs:
"RSRC2: 65117
SRSF2: 6427"
Figure legend: "Immunohistochemistry performed on 12 month-old THY-Tau22 mice showing AT100-positive neurons (green), CBP-positive nuclei (red) and DAPI-stained nuclei (blue) in the CA1 region of the dorsal hippocampus. A representative image is shown with a focus. Scale bars: 40µm and 20µm as noted. (n=5 mice). Arrows depict neurons bearing neurofibrillary tangles and do not display CBP immunostaining. The star depicts a ghost tangle (AT100-positive neuron and no nucleus)"
NCBI gene IDs:
"Tau22: 4137
THY: 21838"
Figure legend: "Western blot analyses perfomed in the dorsal hippocampus of 12 month-old THY-Tau22 mice compared to age-matched controls. NeuN, actin, tubulin and total H2B levels are not changed. Phosphorylated tau on serine 404 (Tau-Ph404) attest for samples from tauopathic mice. Quantification represents the ration of the protein level detected on the total amount of proteins on the membranes, with WT arbitrarily set at 1 (fold induction). Bar graphs are mean ± SEM. n=5-7/group as noted, multiple t tests, CBP * p=0.003 and GFAP **p=0.0001 for THY-Tau vs. WT mice"
NCBI gene IDs:
"Tau: 4137
Tau22: 4137
THY: 21838"
Figure legend: "RNA-sequencing data performed in the hippocampus of 8 month-old mice. Functional analyses (DAVID GOTERM) performed on significantly deregulated genes in THY-Tau22 mice compared to WT mice. Representative genes are presented below. |log2 Fold Change| &gt; 0.2; * indicates FDR&lt;0.05. IEG, Immediate Early Genes. Predicted promoter motif (right) analyses performed with GREAT. Groups: WT mice (n=3), THY-Tau22 mice (n=2)"
NCBI gene IDs:
"Tau22: 4137
THY: 21838"
Figure legend: "Long-term spatial memory testing: Mice were injected 3 times (1 per week) with Vehicle (WT mice saline, WT VEH, n=17), Vehicle (THY-Tau22 mice CSP, 500 μg /mouse, TAU VEH, n=10) or Molecule (THY-Tau22 mice CSP-TTK21, 500 μg/mice TAU MOL, n=13) before training of spatial memory in the Morris water maze (MWM); retention (Probe test) was tested 10 days after the last training session. Acquisition (Escape latencies, seconds) and retention performances (Time in target quadrant, seconds) are shown for the 3 groups of mice. All groups of mice displayed significant acquisition of the platform location (Day effect, F(4,148)= 26.45, p&lt;0.001; Group and Group X Day effects, ns), but only TAU VEH exhibited impaired retention. CSP-TTK21 treatment fully restored the ability of THY-Tau22 mice to remember the platform location. Bar graphs are mean ± SEM. Student's t test to a constant value, # when compared to random (dotted line, 15sec) WT VEH, t(16)=4.6323, #p=0.0002; TAU VEH, t(9)=0.3606, p=0.7267; TAU MOL, t(12)=3.945, #p=0.0019. One-way ANOVA; F(2,37)= 3.55; p= 0.03; * in the different comparisons: TAU MOL vs. TAU VEH, *p=0.0166; WT VEH vs. TAU VEH, *p= 0.040; TAU MOL vs WT VEH, non significant p=0.437). TQ, Target Quadrant; O, Other, corresponds to the mean of the 3 other quadrants"
NCBI gene IDs:
"TAU: 4137
Tau22: 4137
THY: 21838"
Figure legend: "The timeline of GFP-lentivirus and CSP or CSP-TTK21 (one injection, 500 μg/mouse) injection is shown. (Left) The total number of spines was significantly decreased in TAU VEH compared to WT VEH mice. TAU MOL hippocampi showed a significant increase in the total number of spines compared to TAU VEH (One-way ANOVA p=0.0011, post hoc Holm Sidak's multiple comparisons test F(2,243) = 7.03; WT VEH vs. TAU VEH, p &lt; 0.0009; TAU MOL vs. TAU VEH, p=0.0455). (Middle) Based on spine type, the number of head spines (Mushrooms, thins) was significantly lower in both TAU VEH and TAU MOL than in WT VEH controls (One-way ANOVA p=0.0018, post hoc Holm Sidak's multiple comparisons test F(2,243) = 6.467; WT VEH vs. TAU VEH, p=0.0013; WT VEH vs. TAU MOL, p=0.0272. Stubby spine density was significantly increased in TAU MOL compared to TAU VEH mice (One-way ANOVA F(2,243) = 4.311, p = 0.0145, post hoc Holm Sidak's multiple comparisons test: TAU MOL vs. TAU VEH, p=0.0109), as the number of filopodia (One-way ANOVA F(2,243) = 3.845, p = 0.0227, post hoc Holm Sidak's multiple comparisons test TAU MOL vs. TAU VEH, p=0.0179). (Right) Typical images are presented showing a dendrite fragment for each condition. White arrowhead depicts stubby spines. Scale bar, 2 μm. Number of dendritc segments: WT VEH, n=67; TAU VEH, n=93, TAU MOL, n=87; Number of neurons: WT VEH, n=20; TAU VEH, n=28, TAU MOL, n=16; Number of mice: WT VEH, n=2; TAU VEH, n=3, TAU MOL, n=3 "
NCBI gene IDs:
"TAU: 4137"