EMBO/SourceData huggingface dataset:
Figure legend: "(F) IRE1+/+ and IRE1-/- BMDMs were treated with PA (500 µM) for 6 hours and protein lysates were analyzed by western blotting using specific antibodies against pIRE1S742, IRE1, p70S6KT389, p70S6K, pS6S235/236, S6, p4E-BP1S65, 4E-BP1 and β-actin. Western blot quantifications are shown next to the figure (n=3 biological replicates, a representative blot is shown)."
NCBI gene IDs:
"IRE1: 78943"
Figure legend: "IRE1-/- and IRE1+/+ BMDMs were treated with PA (500 µM) or vehicle for 6 hours prior to RNA isolation for miRNA analysis using a micro-array platform. (B) RNA lysates from the same experiment were analyzed by qRT-PCR for miR-2137 expression."
NCBI gene IDs:
"IRE1: 78943
miR-2137: 100316779"
Figure legend: "IRE1-/- and IRE1+/+ BMDMs were treated with PA (500 µM) or vehicle for 6 hours prior to RNA isolation for miRNA analysis using a micro-array platform. (C) Protein lysates were analyzed by western blotting using specific antibodies for pIRE1S742, IRE1 and β-actin (n=3 biological replicates)."
NCBI gene IDs:
"IRE1: 78943"
Figure legend: "IRE1-/- and IRE1+/+ BMDMs were treated with PA (500 µM) or vehicle for 6 hours prior to RNA isolation for miRNA analysis using a micro-array platform. (D) RNA lysates were analyzed by qRT-PCR for sXBP1 expression (n=3 biological replicates)."
NCBI gene IDs:
"IRE1: 78943
XBP1: 22433"
Figure legend: "IRE1-/- and IRE1+/+ BMDMs were treated with PA (500 µM) or vehicle for 6 hours prior to RNA isolation for miRNA analysis using a micro-array platform. (E-F) RNA lysates from the same experiment were analyzed by qRT-PCR for (E) BLOC1S1 and (F) SCARA3 expression (n=3 biological replicates)."
NCBI gene IDs:
"BLOC1S1: 14533
IRE1: 78943
SCARA3: 219151"
Figure legend: "(A-B) BMDMs were treated with PA, steric acid (SA), palmitoleic acid (PAO) or oleic acid (OA) (500 µM) for 16 hours. RNA lysates were analyzed by qRT-PCR for the expression of (A) miR-2137 and (B) sXBP1 (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779
XBP1: 22433"
Figure legend: "(C, D) (C) C57BL/6 (WT) and (D) Apoe-/- mice were fed with chow or WD for 16 weeks followed by peritoneal macrophage isolation. RNA lysates were analyzed by qRT-PCR for miR-2137 expression (WT; n=4-4 mice per group, Apoe-/-; n=5-5 mice per group)."
NCBI gene IDs:
"Apoe: 11816
miR-2137: 100316779"
Figure legend: "(E-F) Apoe-/- mice were fed with chow or WD for 12 weeks and injected with 4µ8c (10 mg/kg/day) or vehicle (DMSO) in the final 6 weeks of the diet (n=5-7 mice per group): (E) RNA lysates from bone marrow were analyzed by qRT-PCR for miR-2137 expression (n=5-7 mice per group). (F) Total RNA isolated from the aortic root plaques was analyzed by qRT-PCR for miR-2137 expression (n=5-7 mice per group)."
NCBI gene IDs:
"Apoe: 11816
miR-2137: 100316779"
Figure legend: "(A) BMDMs were transfected with scrambled (Scr) or IRE1-specific siRNA (40 nM) and treated with palmitate (PA, 500 µM) or vehicle for 6 and 9 hours (hr). RNA lysates were analyzed by qRT-PCR for miR-2137 expression (n=3 biological replicates)."
NCBI gene IDs:
"IRE1: 78943
miR-2137: 100316779"
Figure legend: "(B) BMDMs were treated with PA (500 µM) and 4µ8c (100 µM) or vehicle for 9 hours. RNA lysates were analyzed by qRT-PCR for miR-2137 expression (n=3 biological replicates). (C) BMDM cells were treated with tunicamycin (Tun; 5 µg/ml) and 4µ8c (100 µM) or vehicle (DMSO) for 9 hours. RNA lysates were analyzed by qRT-PCR for miR-2137 expression (n=3 biological replicates). "
NCBI gene IDs:
"miR-2137: 100316779"
Figure legend: "(D) IRE1-/- MEFs were transfected with vector (control), WT-IRE1 or K907A-IRE1 (RNase dead mutant) plasmids for 24 hours, followed by PA (500 µM) treatment for 9 hours. RNA lysates were analyzed by qRT-PCR for miR-2137 expression (n=3 biological replicates)."
NCBI gene IDs:
"IRE1: 78943
miR-2137: 100316779"
Figure legend: "(E) BMDMs were transfected with scrambled or XBP-1 specific siRNA (70 nM) for 24 hours, followed by treatment with PA (500 µM) for 16 hours. RNA lysates were analyzed by qRT-PCR for miR-2137 expression (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779
XBP-1: 22433"
Figure legend: "(F) XBP1-/- MEFs were transfected with empty vector or sXBP1 plasmid for 24 hours, followed by PA (500 µM) treatment for 9 hours. RNA lysates were analyzed for miR-2137 expression by qRT-PCR (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779
XBP1: 22433"
Figure legend: "(G) IRE1 cleavage assay performed using synthetic pre-miR-2137 (50 nM) as substrate with active, recombinant IRE1 (100 ng) at 37℃ for 2 hours, followed by sample separation in Urea-PAGE and detection with SYBR gold staining. M indicates micro RNA marker."
NCBI gene IDs:
"miR-2137: 100316779"
Figure legend: "(H-I) BMDMs isolated from WT (C57BL6) and DICER-/- mice were treated with PA (500 µM) for 12 hours. RNA lysates were analyzed by qRT-PCR for (H) miR-2137 and (I) sXBP1 expression (n=3 biological replicates)."
NCBI gene IDs:
"DICER: 192119
miR-2137: 100316779
XBP1: 22433"
Figure legend: "(B) HEK293 cells were co-transfected with an INPPL1 3` UTR-luciferase plasmid and scrambled miRNA mimic (40 nM), miR-2137 mimic (40 nM) or miR-205 mimic (40 nM) for 24 hours. Protein lysates were analyzed for luciferase activity and normalized to secreted embryonic alkaline phosphatase (SEAP) reporter activity (n=3 biological replicates)."
NCBI gene IDs:
"alkaline phosphatase: 
luciferase: 
INPPL1: 16332
miR-205: 406988
miR-2137: 100316779"
Figure legend: "(C) BMDMs were transfected with scrambled miRNA mimic (40 nM), miR-2137 mimic (40 nM) and miR-205 mimic (40 nM) for 48 hours and protein lysates were analyzed by western blotting using specific antibodies for INPPL1 and β-actin. Protein quantifications are displayed in graph next to the blots (n=3 biological replicates)."
NCBI gene IDs:
"miR-205: 406988
miR-2137: 100316779"
Figure legend: "(D) BMDMs were transfected with negative control antagomir (200 nM) or miR-2137 antagomir (200 nM) for 24 hours, followed by PA (500 µM) treatment for 9 hours. Protein lysates were analyzed by western blotting using specific antibodies for INPPL1 and β-actin. Protein quantifications shown in the graph next to blots (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779"
Figure legend: "(A-C) BMDMs were transfected with scrambled miRNA mimic (40 nM), miR-2137 mimic (40 nM) or miR-205 mimic (40 nM) for 24 hours and cells were processed for lipidomics analysis as described in materials and methods section. The ratio of lipids from this analysis is shown for (A) PI(3,4,5)P3 to PIP2.; (B) PI(3,4,5)P3 to PI; (C) PI(3,4,5)P3 to PIP. Data from LC/MS/MS using a Waters Xevo TQ-S MS/MS in MRM mode represent mean values ± SEM of peak areas normalized to internal standards as described in Methods. (n=3 biological replicates)"
NCBI gene IDs:
"miR-205: 406988
miR-2137: 100316779"
Figure legend: "BMDMs were transfected with (D) scrambled miRNA mimic (40 nM) or miR-2137 mimic (40 nM) for 48 hours and protein lysates were analyzed by western blotting using specific antibodies against INPPL1, pAKTS473, AKT, p70S6KT389, p70S6K, pS6S235/236, S6, p4E-BP1S65, 4E-BP1, pmTORS2448, mTOR and β-actin. Protein quantifications are shown next to the western blots (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779"
Figure legend: "BMDMs were transfected (E) with control antagomir (200 nM) or miR-2137 antagomir for 24 hours and protein lysates were analyzed by western blotting using specific antibodies against INPPL1, pAKTS473, AKT, p70S6KT389, p70S6K, pS6S235/236, S6, p4E-BP1S65, 4E-BP1, pmTORS2448, mTOR and β-actin. Protein quantifications are shown next to the western blots (n=3 biological replicates)."
NCBI gene IDs:
"miR-2137: 100316779"
Figure legend: "The presence of GFP-PSF-1 on mitotic chromatin (indicated by white arrows) was monitored by spinning disk confocal microscopy (see Materials and Methods), in GFP-psf-1 mCherry-Histone H2B worms that were fed on bacteria containing a single plasmid expressing the indicated RNAi treatments ('Control' = empty vector). NEB = nuclear envelope breakdown. The scalebars correspond to 5 µm."
NCBI gene IDs:
"mCherry: 
Histone H2B: 175029
psf-1: 187885"
Figure legend: "Similar experiment comparing the impact of RNAi inactivation of lrr-1, tim-1 and tipn-1 upon CMG-MCM-7 ubiquitylation and the association of CUL-2LRR-1 with the worm replisome. Asterisks indicate non-specific bands in the immunoblots."
NCBI gene IDs:
"lrr-1: 173869
tim-1: 176652
tipn-1: 171803"
Figure legend: "Analogous experiment to that in (A), but with worms fed on the indicated proportions of bacteria expressing ubxn-3 RNAi, in combination with 20% bacteria expressing tim-1 RNAi specific for tim-1, plus the required remainder of bacteria containing empty vector (to make a total of 100%)."
NCBI gene IDs:
"tim-1: 176652
ubxn-3: 173407"
Figure legend: "Correlation between IKBKE mRNA levels (log2 ratio) and immune signature (Yoshihara et al, 2013) in the METABRIC transcriptomic dataset from 1981 breast cancer patients (Curtis et al, 2012). Pearson's correlation rho coefficient and significance of difference from slope = 0 (p) are shown. IKBKE gene copy number and mRNA levels (log2 ratio) from the METABRIC transcriptomic dataset (see D) are plotted. Estimated +1 copy number values are shown by the dotted vertical line. No significant correlation by Pearson's correlation rho coefficient and significance of difference from slope = 0 (p) was detected."
NCBI gene IDs:
"IKBKE: 9641"
Figure legend: "IKBKE gene (encoding for IKKε) was deleted in MCF10A cells via CRISPR-Cas9 technology. 3 knockout clones were combined for each cell line. IKKε CRISPR-Cas9 knockout MCF10A cells form less colonies when grown in soft agar with macrophage-conditioned medium for 5 weeks compared to CRISPR-Cas9 control (nontargeting crRNA) cells. Colonies ≥ 50 μm were counted. Error bars represent mean ± SEM from 3 independent experiments (n=3 per condition). Media from two macrophage donors were combined in 1:1 ratio in each experiment. Macrophage donors are indicated as M1A - M1 activated, M2A - M2 activated macrophages."
NCBI gene IDs:
"IKBKE: 9641"
Figure legend: "IKBKE gene (encoding for IKKε) was deleted in MCF10A cells via CRISPR-Cas9 technology. 3 knockout clones were combined for each cell line. 16-days old MCF10A spheroids grown in matrigel/collagen mix were stimulated for 24h with either macrophage-conditioned or control medium. IKKε CRISPR-Cas9 knockout MCF10A cells are less invasive compared CRISPR Control (nontargeting crRNA) cells. Error bars represent mean ± SEM from 3 independent experiments (n=3 per condition). Media from two macrophage donors were combined in 1:1 ratio in each experiment. Macrophage donors are indicated as M1A - M1 activated, M2A - M2 activated macrophages."
NCBI gene IDs:
"IKBKE: 9641"
Figure legend: "Correlation of mRNA levels of the SBP enzyme genes PSAT1 (E), PHGDH (F), PSPH (G) with the immune signature (Yoshihara et al, 2013) in the METABRIC transcriptomic dataset from 1981 breast cancer patient (Curtis et al, 2012)."
NCBI gene IDs:
"PHGDH: 26227
PSAT1: 29968
PSPH: 5723"
Figure legend: "PHGDH gene was deleted in MCF10A cells via CRISPR-Cas9 technology. 3 knockout clones were combined for each cell line. PHGDH CRISPR-Cas9 knockout MCF10A cells form less colonies when grown in soft agar with macrophage-conditioned medium for 5 weeks compared to CRISPR-Cas9 control (nontargeting crRNA) cells. Colonies ≥ 50 μm were counted. Error bars represent mean ± SEM from 3 independent experiments (n=3 per condition). Media from two macrophage donors were combined in 1:1 ratio in each experiment. Macrophage donors are indicated as , M1A - M1 activated, M2A - M2 activated macrophages."
NCBI gene IDs:
"PHGDH: 26227"
Figure legend: "PHGDH gene was deleted in MCF10A cells via CRISPR-Cas9 technology. 3 knockout clones were combined for each cell line. 16-days old MCF10A spheroids grown in matrigel/collagen mix were stimulated for 24h with either macrophage-conditioned or control medium. PHGDH CRISPR-Cas9 knockout MCF10A cells are less invasive compared CRISPR Control (nontargeting crRNA) cells. Error bars represent mean ± SEM from 3 independent experiments (n=3 per condition). Media from two macrophage donors were combined in 1:1 ratio in each experiment. Macrophage donors are indicated as M1A - M1 activated, M2A - M2 activated macrophages."
NCBI gene IDs:
"PHGDH: 26227"
Figure legend: "A-J)Ten per group female C57Bl/6 mice heterozygous for the PyMT-MMTV started high fat diet (HFD) when 6-7 weeks old (prior of mammary tumour development) together with control mice on normal diet (ND; n=10). Wild-type mice on HFD gain more weight compared to ND mice. *p &lt; 0.05 by one-tailed students t-test."
NCBI gene IDs:
"PyMT: "
Figure legend: "A-J)Ten per group female C57Bl/6 mice heterozygous for the PyMT-MMTV started high fat diet (HFD) when 6-7 weeks old (prior of mammary tumour development) together with control mice on normal diet (ND; n=10). Average adipocytes size (major diameter) in mammary fat pads is larger in HFD mice compared to ND mice based on the analysis of at least 100 adipocytes per mice on H&amp;E stained sections. *p &lt; 0.05 by two-tailed students t-test."
NCBI gene IDs:
"PyMT: "
Figure legend: "A-J)Ten per group female C57Bl/6 mice heterozygous for the PyMT-MMTV started high fat diet (HFD) when 6-7 weeks old (prior of mammary tumour development) together with control mice on normal diet (ND; n=10). The number of F4/80+ macrophages in mammary fat pads is increased in HFD mice compared to ND mice. The average of F4/80+ macrophages in at least 10 fields of view per mouse is shown. *p &lt; 0.05 by two-tailed students t-test."
NCBI gene IDs:
"PyMT: "
Figure legend: "A-J)Ten per group female C57Bl/6 mice heterozygous for the PyMT-MMTV started high fat diet (HFD) when 6-7 weeks old (prior of mammary tumour development) together with control mice on normal diet (ND; n=10). Mice were administered amlexanox (17 mM) or vehicle control daily by oral gavage (Reilly et al, 2013). A control group (wild-type mice) was also included to confirm the efficacy of the diet and to control for possible unpredicted interactions of the MMTV-PyMT gene. PyMT-MMTV+/- mice on HFD gain more weight compared to ND mice. *p &lt; 0.05 by one-tailed students t-test. Amlexanox does not affect weight gain either in mice on F) ND or G) HFD."
NCBI gene IDs:
"PyMT: "
Figure legend: "A-J)Ten per group female C57Bl/6 mice heterozygous for the PyMT-MMTV started high fat diet (HFD) when 6-7 weeks old (prior of mammary tumour development) together with control mice on normal diet (ND; n=10). Mice were administered amlexanox (17 mM) or vehicle control daily by oral gavage (Reilly et al, 2013). A control group (wild-type mice) was also included to confirm the efficacy of the diet and to control for possible unpredicted interactions of the MMTV-PyMT gene. Mammary tumours are developing earlier in PyMT-MMTV+/- mice on HFD compared to ND mice. Amlexanox does not affect tumour development in PyMT-MMTV+/- mice on ND, but delays tumour formation in J) HFD mice."
NCBI gene IDs:
"PyMT: "
Figure legend: "B RIPK1 is dispensable for apoptosis  induction by LBW242  and VCR. LOUCY  cells were transfected with a non-targeting siRNA (neg-siRNA) or a RIPK1-specific siRNA. 6h post transfection, cells were treated with the indicated drugs and apoptosis  was measured 40h thereafter. Mean ± SD of 3 independent experiments is shown; *** p&lt;0.001 by unpaired Student's t-test. Knockdown (KD) was verified by Western blot at the time of apoptosis  measurement and KD efficiency calculated compared to the control. One representative immunoblot  out of 3 biological replicates is shown."
NCBI gene IDs:
"RIPK1: 8737"
Figure legend: "D KD of NEMO  does not affect the SM-mediated sensitization to chemotherapy-induced apoptosis. Experiment was performed as described in B, except that an siRNA targeting NEMO  was used. Mean ± SD of 3 independent experiments is shown; * p&lt;0.05 by unpaired Student's t-test."
NCBI gene IDs:
"NEMO: 8517"
Figure legend: "F KD of RelB does not inhibit apoptosis. Experiment was performed as described in B, except that an siRNA targeting RelB was used. Mean ± SD of 3 independent experiments is shown; differences are not significant, by unpaired Student's t-test."
NCBI gene IDs:
"RelB: 5971"
Figure legend: "A Expression  of XIAP  mRNA in samples obtained from BM  of healthy individuals (Ctrl, n=10) or patients  with AML  (n=517) or ALL  (n=306). Data are expressed as median, 25th and 75th percentile, whiskers indicate min/max; ** p&lt;0.01; **** p&lt;0.0001 by Games-Howell post-hoc test."
NCBI gene IDs:
"XIAP: 331"
Figure legend: "Parental and XIAP  over-expressing  NALM-6 cells (C) were treated with VCR apoptosis  (Annexin-V  / PI) was measured at the indicated  time points (C) by  flow cytometry. Mean ± SEM of 3 independent experiments is shown; **** p ≤0.0001 by unpaired Student's t-test. XIAP   protein level was analyzed in parental and XIAP-overexpressing NALM-6 cells by protein immunoassay (Simple Western); Actin  was used as loading control. One representative immunoassay out of 3 independent experiments is shown."
NCBI gene IDs:
"XIAP: 331"
Figure legend: "D Parental and XIAP  over-expressing REH  cells (D) were treated with VCR  or LBW242; apoptosis  (Annexin-V / PI) was measured after 72h (D) by  flow cytometry. Mean ± SEM of 3 independent experiments is shown; **** p ≤0.0001 by unpaired Student's t-test. XIAP  protein level was analyzed by protein immunoassay (Simple Western); Actin  was used as loading control. One representative immunoassay out of 3 independent experiments is shown."
NCBI gene IDs:
"XIAP: 331"
Figure legend: "B shRNA expression control. Equal expression levels of  ds-RED in the sh-CTRL/mTagBFP and sh-XIAP/eGFP  subpopulation indicates identical expression levels of control and XIAP  shRNA. One representative histogram out of 3 independent experiments is shown."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "C Expression of IAP. Western blot analysis of XIAP  in NALM-6 cells expressing sh-CTRL/mTagBFP or sh-XIAP/eGFP  under the SFFV  promoter; GAPDH  was used as loading control. One representative immunoblot  out of 3 independent experiments is shown."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "D  Western blot analysis of NALM-6  cells with moderate (69%, EF1α  promoter) or strong (92%, SFFV   promoter) KD of XIAP; GAPDH  was used as loading control. The XIAP  KD levels (% KD) were quantified relative to sh-CTRL. One representative immunoblot  out of 3 independent experiments is shown."
NCBI gene IDs:
"EF1α: 1915
XIAP: 331"
Figure legend: "F Moderate knockdown of XIAP  sensitizes towards chemotherapy. NALM-6 cells expressing sh-CTRL/mTagBFP or sh-XIAP/eGFP were mixed at a 1:1 ratio and injected into groups of mice. 4 days after injection, mice  were either treated with PBS  (n=4), or with Vincristin  (VCR, n=8), 0.3mg/kg i.v. once a week for 3 consecutive weeks. 7 days after last treatment, mice  were sacrificed, and relative proportion of sh-CTRL and sh-XIAP  subpopulations re-isolated from the BM  was quantified by flow cytometry. Data are depicted as mean ± SEM of all mice analyzed with **** p ≤0.0001 by unpaired Student's t-test with Welch's correction."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "G Strong knockdown of XIAP  impairs  NALM-6 growth in vivo. NALM-6  cells expressing sh-CTRL/mTagBFP or sh-XIAP/eGFP were mixed at a 1:1 ratio and injected into groups of mice  (n=8). 38 or 46 days after injection, mice  were sacrificed, and relative proportion of sh-CTRL and sh-XIAP subpopulations re-isolated from the BM was quantified by flow cytometry. Data are depicted as mean ± SEM of all mice analyzed with **** p ≤0.0001 by unpaired Student's t-test."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "H PARP  cleavage in NALM-6 cells with strong XIAP  inhibition. NALM-6 cells were lentivirally  transduces with sh-CTRL or sh-XIAP  (SFFV). 4 days later, cells were lysed and relative cleavage level of PARP  (%) was analyzed by protein immunoassay (Simple Western). Mean ± SD of 3 independent experiments is shown; ** p &lt;0.01 by unpaired Student's t-test."
NCBI gene IDs:
"XIAP: 331"
Figure legend: "I XIAP  reconstitution in NALM-6 cells. NALM-6 cells were transduced with shCTRL, shXIAP.1 alone or shXIAP.1 together with the XIAP  overexpression vector. At day 0, transduced cells were mixed in a 1:1 ratio with parental (untransduced) NALM-6  cells. Distribution of the fluorochrome marker was used as a readout to determine growth  variations of the respective subpopulation over time. Mean ± SD of 3 independent experiments is shown; ** p &lt;0.01 by unpaired Student's t-test."
NCBI gene IDs:
"XIAP: 331"
Figure legend: "XIAP  plays an essential role for  ALL-265 growing in mice. Experimental scheme and stability of XIAP  knockdown over passaging. ALL-265 PDX cells produced as described in A were mixed at a 1:1 ratio and injected into mice  (P1, n=4). At clinical signs of illness, cells were isolated and re-injected into next recipient mice  (P2, n=4). XIAP  expression levels of P1 and P2 PDX cells expressing sh-CTRL/mTagBFP or sh-XIAP/eGFP  were analyzed by Western blot  at the end of each passage; GAPDH  was used as loading control (B)."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "XIAP  plays an essential role for ALL-265 growing in mice.  ALL-265 PDX cells produced as described in A were mixed at a 1:1 ratio and injected into mice  (P1, n=4). At clinical signs of illness, cells were isolated and re-injected into next recipient mice  (P2, n=4). Relative proportion of ALL-265 GEPDX cells expressing sh-CTRL/mTagBFP or sh-XIAP/eGFP  re-isolated from the BM  at the end of each passage was quantified by  flow cytometry  (C). Data are depicted as mean ± SEM of all mice  analyzed (n=4, each passage) with **** p&lt;0.0001 by unpaired Student's t-test."
NCBI gene IDs:
"eGFP: 
mTagBFP: 
XIAP: 331"
Figure legend: "D Dose-response  relationship between strength of XIAP  knockdown and growth  disadvantage;  ALL-265 PDX cells of a representative mouse  were analyzed separately for dsRED/shRNA high and low expressing cells."
NCBI gene IDs:
"dsRED: 
XIAP: 331"
Figure legend: "One-week-old mosquitoes  were thoracically  infected with DENV2-Asian I  (A), DENV2-Asian American (B), DENV3-I mutants, respectively. A. aegypti mosquitoes  were infected intrathoracically with 10 p.f.u. of DENV. The DENV  viral loads were assessed at 3 days postinfection via  qRT-PCR and were normalized against  A. aegypti actin. Data information: one dot represents one mosquito, and the horizontal lines represent the median of the results. data are presented as mean ± SEM. The data were analyzed statistically using the Mann-Whitney test The P value represents a comparison between the mutants and corresponding WT within genotypes. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s., not significant (p &gt; 0.05)."
NCBI gene IDs:
"actin: 5572986"
Figure legend: "One-week-old mosquitoes  were thoracically  infected with DENV4-I  (D) mutants, respectively. A. aegypti mosquitoes  were infected intrathoracically with 10 p.f.u. of DENV. The DENV viral loads  were assessed at 3 days postinfection via qRT-PCR  and were normalized against  A. aegypti actin. Data information: one dot represents one mosquito, and the horizontal lines represent the median of the results. data are presented as mean ± SEM. The data were analyzed statistically using the Mann-Whitney test The P value represents a comparison between the mutants and corresponding WT within genotypes. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, n.s., not significant (p &gt; 0.05)."
NCBI gene IDs:
"actin: 5572986"