EMBO/SourceData huggingface dataset:
Figure legend: "D/E. Effect of Rab7 GTPase activity status on its association with the peripheral SKIP compartment. D. Left panels: Representative confocal images of fixed HeLa cells expressing GFP-Rab7 or its mutants Q67L or T22N (green) together with HA-RILP (red) and Myc-SKIP (blue), immunolabeled against the indicated epitope tags. Right panels: Schematic overview per condition. E. Colocalization (Mander's overlap) between the indicated protein pairs, n Rab7=5, n QL=5, n TN=7 images (2≥ cells per image) analysed from 2 independent experiments. F. Graphical summary of Rab7 removal from the SKIP compartment. "
NCBI gene IDs:
"GFP: 
Rab7: 478945"
Figure legend: "B. Representative confocal images of fixed HeLa cells depleted of TBC1D2, 1D5, or 1D15 using siRNA oligo pools and immunolabeled against CD63 (white), scale bar: 10µm. C. Plot of CD63 pixel distribution as a function of TBC1D2/1D5/1D15 depletion expressed as fractional distance along a straight line from center of nucleus (0) to the plasma membrane (1.0), number of (pixels) plotted given above each scatter, n≥8 cells per condition analysed from 2 independent experiments."
NCBI gene IDs:
"1D15: 64786
TBC1D2: 55357
1D5: 9779"
Figure legend: "D/E. Rescue of TBC1D15 depletion phenotype. D. Representative confocal images of fixed HeLa cells transfected with either control siRNA (siC) or oligo #3 targeting TBC1D15 (siTBC1D15) and ectopically expressing either GFP-EV, GFP-TBC1D5 or siRNA-resistant GFP-TBC1D15res (green), immunolabeled for CD63 (magenta), scale bar: 10µm. E. Quantification of rescue expressed as % cells (average) in the population exhibiting one of 3 phenotypes: clustered, dispersed or intermediate; total numbers of cells analysed per condition appear above each bar, n=3 independent experiments."
NCBI gene IDs:
"GFP: 
TBC1D15: 64786
TBC1D5: 9779"
Figure legend: "Effects of TBC1D15 depletion on the morphology and dynamics of late organelles. F. Representative electron micrographs of fixed HeLa cells transfected with either control siRNA (siC) or a pool of oligos targeting TBC1D15 (siTBC1D15) are shown, scale bars as indicated."
NCBI gene IDs:
"TBC1D15: 64786"
Figure legend: "G. Analysis of late compartment dynamics as a function of TBC1D15 depletion. Tracks followed by SiR-lysosome-positive vesicles during a time-lapse lasting 255 sec (5 s per frame), with highest displacement rates for each track depicted on a rainbow colour scale (blue: immobile; red: maximum mobility per time interval). Zoom insets (3x) highlight select peripheral (PP) and perinuclear (PN) regions, scale bar: 10µm EV10). H. Quantification of high motility fraction (% tracks with displacement rates above 0.9µm/sec), n siC=27, n si1D15=37 images (2≥ cells per image) analysed from 2 independent experiments. Effectiveness of TBC1D15 (si1D15) depletion is confirmed by immunoblot against endogenous TBC1D15 (e1D15) following its immunoprecipitation (IP), TL: total lysate."
NCBI gene IDs:
"1D15: 64786
TBC1D15: 64786"
Figure legend: "I. FRAP of GFP-Rab7 in live HeLa cells transfected with either control siRNA (siC, black line) or a pool of oligos targeting TBC1D15 (si1D15, red line). Plotted is average GFP-Rab7 signal recovery during 200 s following bleaching, expressed as % of pre-bleach signal, n=3 bleach regions per sample."
NCBI gene IDs:
"1D15: 64786
TBC1D15: 64786"
Figure legend: "Effects of depleting known GAPs for Rab7 on late compartment segregation mediated by RILP and SKIP. A. Representative confocal images of fixed HeLa cells transfected with either control siRNA (siC), two different siRNA oligos targeting TBC1D15 (#1 and #3) or oligo pool targeting TBC1D17 and ectopically expressing GFP-Arl8b (green) in combination with HA-RILP (cyan) and Myc-SKIP (red), immunolabeled against endogenous Rab7 (eRab7, magenta) and the indicated epitope tags. Cell and nuclear boundaries are demarcated with solid and dashed lines respectively, zoom insets (3.5x) highlight select peripheral (PP) and perinuclear (PN) cell regions, scale bar: 10µm. "
NCBI gene IDs:
"TBC1D15: 64786
TBC1D17: 79735"
Figure legend: "E. Effect of TBC1D15 depletion on Rab7-dependent MHC-II receptor trafficking to the SKIP-positive compartment, n siC=21, n si1D15=21 cells analysed from 3 independent experiments"
NCBI gene IDs:
"1D15: 64786
TBC1D15: 64786"
Figure legend: "Representative confocal images of either control (siC) Hela cells harbouring endogenous TBC1D15 tagged with GFP (Ge1D15, green) or those depleted of FIS1 using a pool of siRNA oligos, fixed and immunolabeled for the mitochondrial marker TOM20 (blue) in combination with either endogenous A. Rab7 Zoom insets (2.5x) highlight select cell regions, arrowheads point to vesicles positive for TBC1D15 and negative for TOM20 (yellow)."
NCBI gene IDs:
"FIS1: 51024"
Figure legend: "Representative confocal images of either control (siC) Hela cells harbouring endogenous TBC1D15 tagged with GFP (Ge1D15, green) or those depleted of FIS1 using a pool of siRNA oligos, fixed and immunolabeled for the mitochondrial marker TOM20 (blue) in combination with either endogenous B. CD63 (red). Zoom insets (2.5x) highlight select cell regions, arrowheads point to vesicles positive for TBC1D15 and negative for TOM20 (yellow)."
NCBI gene IDs:
"FIS1: 51024"
Figure legend: "Effect of FIS1 on late compartment distribution. C. Representative confocal images of fixed HeLa cells depleted of FIS1 using a siRNA oligo pool and immunolabeled against CD63 (white)."
NCBI gene IDs:
"FIS1: 51024"
Figure legend: "D. Plot of CD63 pixel distribution as a function of FIS1 depletion expressed as fractional distance along a straight line from center of nucleus (0) to the plasma membrane (1.0), number of (pixels) plotted given above each scatter, n siC=14, n siFis1=17 cells analysed from 2 independent experiments."
NCBI gene IDs:
"FIS1: 51024
Fis1: 51024"
Figure legend: "E. Colocalization of SKIP with endogenous Rab7 (Mander's overlap) in response to FIS1 depletion, n siC=13, n siFis1=9 images (3≥ cells per image) analysed from 2 independent experiments"
NCBI gene IDs:
"FIS1: 51024
Fis1: 51024"
Figure legend: "F. Validation of FIS1 depletion efficiency assayed by qPCR and expressed as fraction FIS1 mRNA remaining relative to siC, n=3 independent experiments."
NCBI gene IDs:
"FIS1: 51024"
Figure legend: "G-J. Recruitment of endogenous TBC1D15 to the SKIP compartment. G. Left panels: representative confocal images of fixed HeLa cells harbouring endogenous TBC1D15 tagged with GFP (G-e1D15, green) and ectopically expressing either empty vector, HA-SKIP or PLEKHM1-HA (red), immunolabeled against HA. Zoom insets (2.7x) highlight select peripheral (PP) and perinuclear (PN) cell regions. Right panels: pixel plots of endogenous GFP-TBC1D15 (green line) and HA signals (red line) corresponding to the dashed white lines in G. H-J. Colocalization (Mander's overlap) between the indicated pairs of proteins, n≥5 images, 3≥ cells per image, analysed per condition from 2 independent experiments"
NCBI gene IDs:
"HA: 
PLEKHM1: 9842
SKIP: 23207"
Figure legend: "Effects of SKIP KMI motif on HOPS complex recruitment. A. Analysis of SKIP/HOPS interactions by co-IP of RFP/mCherry-HOPS subunits with Myc-SKIP versus the KMI motif mutant AAA from HEK293T cells using RFP-trap beads. Representative immunoblots against RFP and Myc are shown, IP: immunoprecipitation, TL: total lysate. B. Quantification of SKIP/HOPS interactions, n=3 independent experiments. Top graph: fold change in binding of HOPS subunits to AAA mutant relative to wild type (WT) SKIP (1.0), significance: one-way ANOVA test (relative to VPS41). Bottom graph: Co-IP per HOPS subunit relative to VPS39, significance: one-way ANOVA test (relative to VPS39), * p&lt;0.05, *** p&lt;0.001, ns: not significant."
NCBI gene IDs:
"SKIP: 23207"
Figure legend: "D. Representative confocal images of fixed HeLa cells ectopically expressing Myc-SKIP, KMI motif mutant (AAA) or RUN domain truncation mutant (∆RUN) (red) together with RFP-VPS39 (green), immunolabeled for endogenous VPS11 (eVPS11, blue)."
NCBI gene IDs:
"Myc: 
SKIP: 23207"
Figure legend: "Effect of HOPS over-expression on recruitment of TBC1D15 to the SKIP compartment. E. Representative confocal images of fixed HeLa cells harbouring endogenous GFP-TBC1D15 (Ge1D15, green) and ectopically expressing HA-SKIP (red) together with mCh-VPS18 or RFP-VPS39 (white), immunolabeled for endogenous VPS18 or VPS11 (blue), as indicated"
NCBI gene IDs:
"mCh: 
RFP: 
VPS18: 57617
VPS39: 23339"
Figure legend: "F-I. Colocalization of SKIP and HOPS complex with TBC1D15 as a function of HOPS subunit over-expression. F/H. Mander's overlap between F. SKIP or H. eVPS18 and endogenous TBC1D15, n≥5 images (2≥ cells per image) analysed per condition from 2 independent experiments, significance: one-way ANOVA test (relative to EV), ** p&lt;0.01, *** p&lt;0.001, ns: not significant. G/I. Mander's overlap between G. SKIP or I. eVPS11 and endogenous TBC1D15 as a function VPS18 overabundance, with or without FIS1 depletion (siFIS1), n≥3 images (2≥ cells per image) analysed per condition from 2 or more independent experiments."
NCBI gene IDs:
"FIS1: 51024
VPS18: 57617"
Figure legend: "A/B. In situ SKIP/TBC1D15 complex formation assayed using proximity-based biotin ligation (BioID). A. Neutravidin precipitates (PD) from biotin-treated HEK293T cells ectopically expressing GFP-TBC1D15 (GFP-1D15) together with HA-BioID-SKIP or HA-BioID-EV. Representative immunoblots against GFP, HA and VPS18 , TL: total lysate. B. Quantification of biotinylation of GFP-TBC1D15 (top graph) and endogenous VPS18 (eVPS18, bottom graph) by BioID-SKIP, expressed relative to BioID-EV (1.0), n=4 independent experiments."
NCBI gene IDs:
"GFP: 
HA: 
SKIP: 23207
1D15: 66687
TBC1D15: 64786"
Figure legend: "C. Analysis of TBC1D15/HOPS interactions by co-IP of RFP/mCherry-HOPS subunits with GFP-TBC1D15 from HEK293T cells using GFP-trap beads. Left panel: schematic of HOPS complex composition, with subunits tested marked in yellow. Right panel: representative immunoblots against GFP and RFP; IP: immunoprecipitation, TL: total lysate."
NCBI gene IDs:
"TBC1D15: 66687"
Figure legend: "Effects of VPS18 depletion on the recruitment of TBC1D15 to the SKIP complex. D. Representative confocal images of fixed HeLa cells harbouring endogenous GFP-TBC1D15 (G-e1D15, green), transfected with either control siRNA (siC) or oligo pool targeting VPS18 (siVPS18) and ectopically expressing HA-SKIP (red), immunolabeled against endogenous VPS18 (eVPS18, blue) and HA, scale bar: 10µm. Cell and nuclear boundaries are demarcated with solid and dashed lines respectively, zoom insets (3.5x) highlight select peripheral (PP) and perinuclear (PN) cell regions. E. Colocalization (Mander's overlap) between SKIP and endogenous TBC1D15 as a function of VPS18 depletion, n siC=9, n siVPS18=11 images (2≥ cells per image) analysed from 2 independent experiments."
NCBI gene IDs:
"VPS18: 57617"
Figure legend: "F. Neutravidin precipitates (PD) from biotin-treated HeLa cells ectopically expressing GFP-TBC1D15 (GFP-1D15) together with BioID-SKIP or BioID-EV. Representative immunoblots against GFP, HA and VPS18 are shown, TL: total lysate. G. Quantification of GFP-TBC1D15 biotinylation by BioID-SKIP over BioID-EV in response to VPS18 depletion expressed as % of control (siC), n=4 independent experiments performed in HeLa or HEK293T cells. H. Quantification of VPS18 protein abundance from experiments in G, n=4."
NCBI gene IDs:
"SKIP: 23207
VPS18: 57617"
Figure legend: ". Effect of TBC1D15 activity on Rab7 displacement from the SKIP compartment. Colocalization (Mander's overlap) between Rab7 and TBC1D15 (WT or R417A mutant) in the presence of either SKIP or PLEKHM1 combined with the indicated VPS proteins, n≥ 5 images (2≥ cells per image) analysed from 2 independent experiments"
NCBI gene IDs:
"PLEKHM1: 9842
SKIP: 23207
TBC1D15: 64786"
Figure legend: "B HCT116  and SW480  cells with GDH1  knockdown were incubated under normoxia  and hypoxia, respectively. Then, cell viability  was assessed by trypan blue staining. (n=3). Data information: Data are mean ± SD from the biological replicates (B Statistics: two-way ANOVA with Tukey's HSD post hoc test (B)"
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "F HIF1A  gene transcription was slightly impaired after GDH1  depletion. The cells in Figure 1B were collected after incubation under normoxia  or hypoxia, and the HIF1A  mRNA level was analysed via quantitative real-time PCR (qRT‒PCR). (n=3; ns, not significant). Data information: Data are mean ± SD from the biological replicates Statistics: unpaired two-tailed student's t-test"
NCBI gene IDs:
"GDH1: 2746
HIF1A: 3091"
Figure legend: "H Tumour formation study. HCT116-shNT and HCT116-shGDH1 cells were injected into the  left groin  of nude mice. Solid tumours were stained  with an antibody against Ki67  (Scale bars: 50 μm). (n=3) (H). Data information: Data are mean ± SD from the biological replicates Statistics: unpaired two-tailed student's t-test"
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "(E) H&amp;E staining  of colon cancer tumours from GDH1IEC+/+ and GDH1IEC-/- mice  (scale bar, 200 μm). F F4/80 staining  in a noninflamed portion of the colon  after AMO/DSS  treatment (scale bar, 50 μm). G ZO-1 staining  in colon  sections of mice  treated with sequential AMO/DSS  treatment (scale bar, 200 μm)."
NCBI gene IDs:
"GDH1: 14661"
Figure legend: "I Survival  percentages of  GDH1IEC+/+ mice  (n=11) and GDH1IEC-/- mice  (n=9) during progression of the inflammatory cancer  switch. Data information: Statistics: log-rank test (I)."
NCBI gene IDs:
"GDH1: 14661"
Figure legend: "K Both HIF1α  protein levels and AMPK  activity were assessed in the intestine  of  GDH1IEC-/- mice  after AOM/DSS  treatment using the indicated antibodies."
NCBI gene IDs:
"GDH1: 14661"
Figure legend: "E HCT116  or SW480  cells containing WT or GDH1  with K503/K527R mutations were incubated under normoxia  and hypoxia, respectively. GDH1-FLAG  was enriched, and an antibody against pan-lysine acetylation  was used to test GDH1-lysine acetylation."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "H GDH1-depleted cell lines were constructed and then rescued with the expression of wild-type GDH1  or K503/K527R mutant GDH1."
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "F HCT116  or SW480  cells were depleted of endogenous GDH1  and rescued with SFB-tagged rGDH1 WT, K503Q or K503R. rGDH1-SFB  proteins were enriched using M2-FLAG  beads to determine the Vmax  of αKG  production (F). (n=3). Data information: Data are mean ± SD from the biological replicates Statistics: one-way ANOVA with Tukey's HSD post hoc test"
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "A, B Immunoblot  of the HIF1α   protein level under hypoxia  for 6 hours when enzymes related to the αKG  level were overexpressed in cells, including GDH1  (A). Relative αKG  levels in contrast to the control (B). (n=3). Data information: Data are mean ± SD from the biological replicates (B Statistics: one-way ANOVA with Tukey's HSD post hoc test (B"
NCBI gene IDs:
"GDH1: 2746"
Figure legend: "I EGLN1  activity test. His-GDH1  with K503 or K527 acetylation  (AcK503 or AcK527) and mimic acetylation  at K503 or K527 (K503Q or K527Q) were purified from  E. coli. Then, His-GDH1  was incubated for 1 hour with HA-EGLN1(I). EGLN1  activity was tested by converting αKG  to succinate. (n=3). Data information: Data are mean ± SD from the biological replicates Statistics: one-way ANOVA with Tukey's HSD post hoc test"
NCBI gene IDs:
"His: "
Figure legend: "the mRNA expression  levels of the HIF1α  target genes GLUT1, HK1, PGK1  and CCND1  (M) in the indicated cell lines under hypoxia. (n=3). Data information: Data are mean ± SD from the biological replicates Statistics: one-way ANOVA with Tukey's HSD post hoc test"
NCBI gene IDs:
"CCND1: 595
HK1: 3098
PGK1: 5230
GLUT1: 6513"
Figure legend: "P HIF1α  transactivation was examined in the indicated cell lines under hypoxia  with or without treatment with the HIF1α  transactivation inhibitor 2-MeOE2 (1 μM) for 12 hours. (n=3). Data information: Data are mean ± SD from the biological replicates P). Statistics: one-way ANOVA with Tukey's HSD post hoc test , P)"
NCBI gene IDs:
"HIF1α: 3091"
Figure legend: "D, The levels of both AcK503 and AcK527 were analysed when cells were transfected with small interfering RNA (siRNA) targeting TIP60, PCAF, CBP  or p300  (D)"
NCBI gene IDs:
"CBP: 1387
p300: 2033
PCAF: 8850
TIP60: 10524"
Figure legend: "F HCT116  and SW480  cells were transfected with or without FLAG-p300. The p300-associated proteins were enriched with M2-FLAG  beads and subjected to immunoblot  analysis using antibodies against GDH1, GDH1-K503 or GDH1-K527 acetylation."
NCBI gene IDs:
"FLAG: 
p300: 2033"
Figure legend: "K The HIF1A  gene was transiently knocked down, and HCT116  cells were cotransfected with FLAG-p300  and HA-GDH1  for a Co-IP assay."
NCBI gene IDs:
"FLAG: 
HA: 
p300: 2033
GDH1: 2746
HIF1A: 3091"
Figure legend: "L EDC-3- and NTL-2-foci lose their specific localization in the vicinity of mitochondria upon genetic inhibition of mrps-5, top: EDC-3-foci, bottom: NTL-2-foci (n=3 independent experiments)."
NCBI gene IDs:
"mrps-5: 179721"
Figure legend: "A Confocal images of young adult animals expressing mitochondria-targeted GFP in body wall muscle cells upon dcap-2 or ntl-2 RNAi treatment (right panels indicate higher magnification images of inlays in left panels; right panel scale bar, 10μm). Images were acquired using a X40 objective lens (n=3 independent experiments)."
NCBI gene IDs:
"dcap-2: 178321
ntl-2: 173773"
Figure legend: "D Quantification of intestinal mitochondrial mass upon knockdown of either dcap-2 or ntl-2, in animals shown in Fig 2B &amp; C. (n=3 independent experiments with at least 30 animals/experiment; ****P&lt; 0.0001; one-way analysis of variance (ANOVA)."
NCBI gene IDs:
"dcap-2: 178321
ntl-2: 173773"
Figure legend: "F Representative images showing the effect of dcap-2 and ntl-2 genetic inhibition on the intestinal cell mitochondrial content in 7-day-old nematodes. (n=3 independent experiments; Images were acquired using a X5 objective lens). G Quantification of the mean GFP fluorescence intensity in the matrix of intestinal mitochondria as shown in Fig 2F (n=3 independent experiments with at least 57 animals/experiment; ****P&lt; 0.0001; one-way analysis of variance (ANOVA))."
NCBI gene IDs:
"dcap-2: 178321
ntl-2: 173773"
Figure legend: "H Quantification of the mtDNA/nDNA ratio under control conditions and upon genetic inhibition of either dcap-2 or ntl-2 (n=2 independent experiments; ***P&lt; 0.001, *P&lt; 0.05; one-way analysis of variance (ANOVA))."
NCBI gene IDs:
"dcap-2: 178321
ntl-2: 173773"
Figure legend: "C Measurement of the promoter activity of the SKN-1 target gene gst-4 upon genetic inhibition of either dcap-2 or ntl-2 in wild-type background, daf-2 mutant background and in a mutant background (skn-1(lax120)) where SKN-1 is constitutively active (n=3 independent experiments with at least 256 animals/experiment; ****P&lt; 0.0001; two-way analysis of variance (ANOVA), followed by Turkey's multiple comparison test)."
NCBI gene IDs:
"daf-2: 175410
dcap-2: 178321
gst-4: 177886
ntl-2: 173773
SKN-1: 177343
skn-1: 177343"
Figure legend: "E Relative expression of skn-1 mRNA in wild-type, DCAP-2- and NTL-2- depleted animals (n=4 independent experiments, P(dcap-2(RNAi)) =0.98, P(ntl-2(RNAi))&gt;0.99; one-way analysis of variance (ANOVA))."
NCBI gene IDs:
"DCAP-2: 178321
dcap-2: 178321
NTL-2: 173773
ntl-2: 173773
skn-1: 177343"
Figure legend: "H Relative expression of aak-2 mRNA in wild-type, DCAP-2- and NTL-2- depleted animals (n=2 independent experiments; P(dcap-2(RNAi)) =0.85, P(ntl-2(RNAi))&gt;0.99; one-way analysis of variance (ANOVA))."
NCBI gene IDs:
"aak-2: 181727
DCAP-2: 178321
dcap-2: 178321
NTL-2: 173773
ntl-2: 173773"
Figure legend: "D Representative images showing an increase in NTL-2-foci upon dcap-2 genetic inhibition (n=3 independent experiments)."
NCBI gene IDs:
"dcap-2: 178321"
Figure legend: "E Quantification of NTL-2 foci upon dcap-2 genetic inhibition (n=3 independent experiments with at least 40 animals/experiment; ****P&lt;0.0001; two-tailed unpaired t-test)."
NCBI gene IDs:
"dcap-2: 178321"
Figure legend: "F Representative images showing an increase in the signal of DCAP-2-foci upon ntl-2 genetic inhibition. Images were acquired using a X40 objective lens (n=3 independent experiments). G Respective quantification (n=3 independent experiments with at least 80 animals/experiment; ****P&lt;0.0001; two-tailed unpaired t-test)."
NCBI gene IDs:
"ntl-2: 173773"
Figure legend: "L Quantification of the percentage of fluorescence recovery after photobleaching (FRAP) to measure the rates of de novo protein synthesis upon genetic inhibition of either ntl-2 or dcap-2 in 6-day-old-adult animals; the protein synthesis inhibitor cycloheximide (500μM) and let-363 genetic inhibition where used as controls. Measurements started after animals have recovered for 2hrs post photobleaching (n=3 independent experiments with at least 50 animals/experiment)."
NCBI gene IDs:
"dcap-2: 178321
let-363: 172167
ntl-2: 173773"
Figure legend: "A Analysis of expression of select MTPTs by real time quantitative PCR (RT-qPCR) following RNA immunoprecipitation (RIP) in anti-GFP isolates from NTL-2::GFP transgenic animals, unc-119(ed3)III (no GFP expression) and pgst-4GFP (used as an additional negative control) counterparts (n=2 independent experiments, ***P&lt; 0.001; one-way analysis of variance (ANOVA)."
NCBI gene IDs:
"GFP: 
gst-4: 177886
NTL-2: 173773
unc-119: 176519"
Figure legend: "B Representative images showing the localization of NTL-2/storage bodies relative to mitochondria upon genetic inhibition of either tomm-20 or akap-1 (green: NTL-2, red: TMRE, a mitochondrial-membrane potential-dependent dye; n=3 independent experiments). Scale bars, 20 µm. Images were acquired using a X63 objective lens. C Quantification of the distances NTL-2/storage bodies acquire from mitochondria upon genetic inhibition of either tomm-20 or akap-1 (n=3 independent experiments with at least 45 animals/experiment; ***P&lt; 0.001; one-way analysis of variance (ANOVA))."
NCBI gene IDs:
"akap-1: 175898
tomm-20: 179691"
Figure legend: "E Immunoblot analysis in mitochondria isolates of whole animal extracts showing the protein levels of NTL-2 contained in the isolate in control conditions and upon genetic inhibition of akap-1, tomm-20, mrps-5 and atp-3; genetic inhibition of ntl-2 is used as a control; NTL-2 protein is detected by an anti-GFP antibody in transgenic animals that contain the NTL-2::GFP protein fusion (n=2 independent experiments). F Quantification of NTL-2 protein bound to mitochondria under control conditions and upon genetic inhibitions shown in E; CTC-1 is used as a loading control for mitochondria (n=2 independent experiments; *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001; one-way analysis of variance (ANOVA) followed by Dunnett's test)."
NCBI gene IDs:
"akap-1: 175898
atp-3: 172195
mrps-5: 179721
ntl-2: 173773
tomm-20: 179691"
Figure legend: "A Percent survival of wild-type, DCAP-2- and NTL-2- depleted animals subjected to heat stress for 5 hrs at 37oC and then counted every 24 hours (n=4 independent experiments with at least 294 animals/experiment). B Percent survival of wild-type, DCAP-2- and NTL-2- depleted animals following paraquat (8mM) administration and counted every 24 hours (n=4 independent experiments with at least 144 animals/experiment). C Percent survival of wild-type, DCAP-2- and NTL-2- depleted animals counted every 24 hours post CCCP (15μM) treatment (n=4 independent experiments with at least 116 animals/experiment)."
NCBI gene IDs:
"DCAP-2: 178321
NTL-2: 173773"
Figure legend: "E Knockdown of ntl-2 shortens the lifespan of wild-type animals, while NTL-2 overexpression extends lifespan."
NCBI gene IDs:
"ntl-2: 173773
NTL-2: 173773"
Figure legend: "A Knockdown of akap-1 extends the lifespan of wild-type animals, while it does not further extend the lifespan of NTL-2 overexpressing animals. B Knockdown of akap-1 reverses the lifespan shortening of ntl-2 mutant worms. C Knockdown of akap-1 ameliorates the lifespan shortening caused by ntl-2 genetic inhibition (double RNAi administered as 1:1 mixture)."
NCBI gene IDs:
"akap-1: 175898
ntl-2: 173773
NTL-2: 173773"