EMBO/SourceData huggingface dataset:
Figure legend: "(A) HeLa cells were transfected with siRNAs for autophagy-related gene ATG5 (siATG5) or with a non-silencing siRNA (siC). Depletion of ATG5, which decreased the levels of the ATG5-ATG12 covalent complex, was confirmed by Western blot. Loading control: transferrin receptor (TfR)."
NCBI gene IDs:
"ATG5: 9474"
Figure legend: "(B) Control or ATG5‐KD cells transfected with UBQLN1 were starved, and the cell viability was assessed as in Fig 1; the graph represents the mean of three experiments±s.e."
NCBI gene IDs:
"ATG5: 9474
UBQLN1: 29979"
Figure legend: "(C) HeLa cells were stably transfected with control (shC) or ATG7 (shATG7) shRNA; depletion was verified by Western blot (loading control: GAPDH)."
NCBI gene IDs:
"ATG7: 10533"
Figure legend: "(D) Control or ATG7‐KD cells transfected with UBQLN1 were starved for 3 or 6 h and assessed for their viability. The graph represents the mean of three experiments±s.e. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; KD, knockdown; Pl, propidium iodide; shRNA, short hairpin RNA; siRNA, short interfering RNA; UBQLN, ubiquilin."
NCBI gene IDs:
"ATG7: 10533
UBQLN1: 29979"
Figure legend: "(C) Control cells (untransfected, right panel) or cells overexpressing Myc‐UBQLN2 and GFP‐LC3 (left panel) were transferred into starvation medium for 2 h. Localization of Myc‐UBQLN2 and GFP‐LC3 was analysed by immunoelectron microscopy using Myc (arrow) and GFP (arrowhead) antibodies coupled to 10 and 15 nm gold particles, respectively. Double‐membrane vacuoles (autophagosomes) in transfected cells (left panel) show increased staining by both antibodies compared with autophagosomes in untransfected cells (right panel). Scale bar, 0.5 μm. BAF A, bafilomycin A1; GFP, green fluorescent protein; UBQLN, ubiquilin."
NCBI gene IDs:
"LC3: 440738///81631///84557
UBQLN2: 29978"
Figure legend: "(C) Starvation‐induced cell death was assessed in cells expressing UBQLN1 or UBQLN1ΔUBA. The graph represents the mean of three experiments±s.e., with *P⩽0.05 (P=0.0017 by one‐way ANOVA test, comparing control, UBQLN1 and UBQLN1ΔUBA). No difference was found between control and UBQLN1ΔUBA at any time point. ANOVA, analysis of variance; GFP, green fluorescent protein; Pl, propidium iodide; UBA, ubiquitin‐associated; UBQLN, ubiquilin."
NCBI gene IDs:
"UBQLN1: 29979"
Figure legend: "(A) Autophagosome subcellular localization during starvation was analysed by microscopy in UBQLN‐ or autophagy‐related gene ATG5‐KD cells expressing GFP‐tagged microtubule associated protein 1 light chain 3 (GFP‐LC3)."
NCBI gene IDs:
"ATG5: 9474"
Figure legend: "B) Crystal structure of the Ago2-miRNA-target RNA ternary complex. Target RNA is colored blue. D) Close-up view of the Ago2-miRNA-target complex shows helix-7 docks into the minor groove of the guide:target duplex, directly contacting base pairs at positions g6 and g7."
NCBI gene IDs:
"target RNA: 
Target RNA: "
Figure legend: "Figure 2. Helix-7 is not required for target recognition. A) Plot of g2-g8 target RNA bound (0.1 nM) versus Ago2-miR122 concentration for wild type (WT), helix-7 double point mutant (MI-AA), and Ηhelix-7 Ago2. Average values from at least three independent experiments ± standard deviation (SD) are plotted. Top panel shows target RNA paired to seed region of the guide RNA (miRNA-122)."
NCBI gene IDs:
"target RNA: 
miR122: 406906"
Figure legend: "B) Dissociation of a 32P-labeled target RNA (0.1 nM) from the Ago2-miR122 complex (1 nM) was monitored in the presence of unlabeled target RNA (100 nM). Fraction of the target RNA bound to Ago2-miR122 is plotted as a function of time for WT and Ηhelix-7 Ago2. Average values from at least three independent experiments ± SD were fit to single exponential decays."
NCBI gene IDs:
"target RNA: 
miR122: 406906"
Figure legend: "Figure 4. Helix-7 discourages non-Watson Crick pairing at g6 and g7. A-C) Plots of target RNA (0.1 nM) bound to Ago2 versus Ago2-miR122 (wild type and Ηhelix-7) concentration for targets with an increasing number of G:U wobble pairs in the seed 3' end. D) Plot of target RNA (0.1 nM) bound to Ago2 versus Ago2-miR122 concentration for a target with mismatches at positions g6-g8. For A-E, average values from at least three independent experiments ± SD are plotted."
NCBI gene IDs:
"target RNA: 
miR122: 406906"
Figure legend: "E) Bar plots of the Kd of wild type (left) Ηhelix-7 (right) Ago2-miR122 for indicated target RNAs. For A-E, average values from at least three independent experiments ± SD are plotted."
NCBI gene IDs:
"target RNAs: 
miR122: 406906"
Figure legend: "Figure 5. ΗHelix-7 Ago2 is sensitive to inhibition by off-target RNAs. A) Ago2-miRNA122 (1 nM) was incubated with a 32P-labeled, seed-matched target RNA (0.1 nM) in the presence of increasing concentrations of unlabeled a competitor RNA (top panel). Fraction target RNA bound to Ago2 is plotted as a function of off-target competitor RNA concentration. B) Fraction of target RNA bound to Ago2 in the presence of increasing concentrations of unlabeled total cellular RNA. For A-B, average values from at least three independent experiments ± SD are plotted."
NCBI gene IDs:
"target RNA: 
target RNAs: 
miRNA122: 406906"
Figure legend: "Figure 6. Helix-7 mutants display reduced target-binding rates. A) Ago2-miR122 complexes were mixed with a 32P-labeled seed matched target RNA; bound and free RNAs were then separated using a filter-binding apparatus at various times. Representative time course for target RNA (0.1 nM) binding to wild type, MI-AA, and Δhelix-7 Ago2 (1 nM). Average values from at least three independent experiments ± SD are plotted."
NCBI gene IDs:
"target RNA: 
miR122: 406906"
Figure legend: "B) Observed binding rates (kon, obs) plotted as a function of Ago2-miR122 concentration. Average values ± SD are plotted."
NCBI gene IDs:
"miR122: 406906"
Figure legend: "C) Arrival time distribution of single Ago2-miRNA complexes binding immobilized targets in a microfluidic chamber. The value is the average of three independent measurements."
NCBI gene IDs:
"miRNA: "
Figure legend: "Figure 7. Structure of a helix-7 Ago2 mutant. A) Superposition of wild type (white) and MI-AA (gray) Ago2. Helix-7 colored yellow; ordered region of the guide RNAs colored in red."
NCBI gene IDs:
"guide RNAs: "
Figure legend: "X-ray analysis of 6-months-old H2-c-fosLTR/Egfrwa/+ and H2-c-fosLTR/Egfrwa/wa littermates. Scale bars: 1cm"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "PET summation images (0-90 min) in horizontal (upper panel) and coronal view (lower panel) depicting [11C]Erlotinib distribution in one H2-c-fosLTR/Egfrwt mouse (M125). Anatomical structures are labelled with arrows (T, tumor; L, liver; H, heart; B, brain). Scale bars: 1cm Concentration-time curves of [11C]Erlotinib in bone tumors in right scapula of three H2-c-fosLTR/Egfrwt mice measured with PET. Broken line indicates threshold for in vitro effect of Erlotinib (1 µM)"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "X-ray analysis of 7-months-old H2-c-fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob littermates. Scale bars: 1cm"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "µPET/CT analysis of 7-months-old H2-c-fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob littermates Standardized uptake values (SUV) of the µPET-tracer Na[18F]F in the pelvic osteosarcoma of 4- and 7-months-old H2-c-fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob mice. n=6 wt, 4 ∆Ob for 4 month time-point, n=6 wt, 3 ∆Ob for 7 month time-point"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "PCNA and cleaved Caspase 3 IHC staining and quantification shown as % positive cells (for PCNA) and as positive cells per mm² (for cleaved Caspase 3) in OS from H2-c-fosLTR/Egfrwt (n=6) and H2-c-fosLTR/Egfr∆Ob (n=5) mice. Scale bars: 100µm"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "Egfr, Ccnd1, c-fos and transgenic c-fos (c-fostg) mRNA expression levels in tumors of H2-c-fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob mice normalized to Tbp. n=17 wt, 14 ∆Ob (Egfr, Ccnd1), n=16 wt, 13 ∆Ob (c-fos), n=16 wt, 14 ∆Ob (c-fostg"
NCBI gene IDs:
"Ccnd1: 12443
Egfr: 13649
c-fos: 14281
Tbp: 21374"
Figure legend: "IHC staining and quantification showing pRSK2 (n=4), pCREB (n=7 wt, 6∆Ob) and c-Fos (n=5) positive cells (%) in OS from H2-c-fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob littermates. Scale bars: 100µm"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "Western Blot analysis of primary OS cells isolated from H2‑c‑fosLTR/Egfrwt and H2-c-fosLTR/Egfr∆Ob mice"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281
c‑fos: 14281"
Figure legend: "c-fos and transgenic c-fos (c fostg) mRNA expression levels in primary H2-c-fosLTR OS cells after 4x in vitro passages, cultured under standard conditions (n=3 independent cell lines)"
NCBI gene IDs:
"c fos: 14281
c-fos: 14281"
Figure legend: "Western Blot analysis of H2-c-fosLTR/Egfrwt OS cells treated for 24h with Erlotinib"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "c-fos and c-fostg mRNA expression levels in H2-c-fosLTR/Egfrwt OS cells treated for 24h with Erlotinib (10µM) or DMSO as control (n=4 independent cell lines)"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "Western Blot analysis of starved H2-c-fosLTR/Egfrwt OS cells, pre-treated with DMSO (1:1000), Afatinib (5µM), GSK2233470 (10µM), Rapamycin (10nM) or U0126 (10µM) for 30 minutes and stimulated with EGF (50ng/ml) as indicated"
NCBI gene IDs:
"Egfr: 13649
c-fos: 14281"
Figure legend: "Western Blot analysis of primary OS cells isolated from a p53f/f Rb1f/f Osx-Cre mouse after 24h Erlotinib treatment"
NCBI gene IDs:
"Cre: 2777477
Rb1: 19645
Osx: 170574
p53: 22059"
Figure legend: "c-fos mRNA expression levels in p53f/f Rb1f/f Osx-Cre OS cells treated for 24h with Erlotinib (10µM) (n=3)"
NCBI gene IDs:
"Cre: 2777477
c-fos: 14281
Rb1: 19645
Osx: 170574
p53: 22059"
Figure legend: "Western Blot analysis of starved p53f/f Rb1f/f Osx-Cre OS cells, pre-treated with DMSO (1:1000) or Afatinib (5µM) for 30 minutes and stimulated with EGF (50ng/ml) as indicated"
NCBI gene IDs:
"Cre: 2777477
Rb1: 19645
Osx: 170574
p53: 22059"
Figure legend: "X-ray analysis of 6-months-old H2-c-fosLTR and H2-c-fosLTR/ColAREG littermates. Scale bars: 1cm"
NCBI gene IDs:
"AREG: 11839
Col: 12842
c-fos: 14281"
Figure legend: "Bone tumor number per mouse at 5-6 months of age (n=22 wt, 13 ColAREG)"
NCBI gene IDs:
"AREG: 11839
Col: 12842"
Figure legend: "Quantification of tumor size in tibiae at 5-6 months of age (n=22 wt, 13 ColAREG)"
NCBI gene IDs:
"AREG: 11839
Col: 12842"
Figure legend: "Alkaline Phosphatase (ALP) levels in the serum at endpoint (age = 5-6 months; n=29 wt, 19 ColAREG)"
NCBI gene IDs:
"AREG: 11839
Col: 12842"
Figure legend: "c-fos mRNA expression levels in osteosarcomas of H2-c-fosLTR (n=11) and H2-c-fosLTR/ColAREG mice (n=14)"
NCBI gene IDs:
"AREG: 11839
Col: 12842
c-fos: 14281"
Figure legend: "Western Blot analysis of bone tumor protein lysates from H2-c-fosLTR and H2-c-fosLTR/ColAREG mice"
NCBI gene IDs:
"AREG: 11839
Col: 12842
c-fos: 14281"
Figure legend: "(A, B) Actively dividing cells labelled by BrdU and Ki67 are reduced in the SGZ of DSCR1 mutants compared to that of wild type mice. Brain sections were prepared 1 day after BrdU injection. The white box area is magnified in the lower panels: DAPI (blue), Ki67 (green), and BrdU (red). Arrow heads indicate BrdU and Ki-67 double positive cells in the SGZ."
NCBI gene IDs:
"DSCR1: 54720"
Figure legend: "(A) miR-124 expression is altered in DSCR1 mutants. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 animals for each condition, *P&lt; 0.01."
NCBI gene IDs:
"DSCR1: 54720"
Figure legend: "(B) Promoter activity of miR-124 in Neuro2 A cells with DSCR1 reduction or overexpression. Firefly luciferase reporter under the control of miR-124 promoter is measured. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 for each condition, *P&lt; 0.05, **P&lt; 0.01."
NCBI gene IDs:
"luciferase: 
miR-124: 70441///268755
DSCR1: 54720"
Figure legend: "(C) Bisulfite sequencing analyses of the miR-124 promoter. Each CpG site is indicated, and the methylation status of two different regions are shown. Open and filled circles represent unmethylated and methylated CpG sites, respectively. The percentage of methylated CpGs among total number of CpGs is also shown."
NCBI gene IDs:
"miR-124: 70441///268755"
Figure legend: "(D) Neuro2A cells containing DSCR1shRNA show increased activity of miR-124 promoter measured. The miR-124 promoter was inserted in front of the luciferase reporter. Site-directed mutation of two methylation sites (31 and 58) in the promoter shows the luciferase activity similar to that of DSCR1 reduction. Values are shown as mean ± SEM, and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 for each condition, *P&lt; 0.05."
NCBI gene IDs:
"luciferase: 
miR-124: 70441///268755
DSCR1: 54720"
Figure legend: "(E) Overexpression of DSCR1 reduced the luciferase activity, while removing all methylation sites in the miR-124 promoter increases it. Values are shown as mean ± SEM, and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 for each condition, *P&lt; 0.05."
NCBI gene IDs:
"miR-124: 70441///268755
DSCR1: 54720"
Figure legend: "(A) DSCR1 binds to TET1 introns. The binding affinity of DSCR1 to the intron 8 of TET1 decreased with increased dosage of U1 snRNA and U2 snRNA."
NCBI gene IDs:
"TET1: 52463"
Figure legend: "(B) DSCR1 missing RNA recognition motif (△RRM) does not bind to the TET1 intron 8."
NCBI gene IDs:
"DSCR1: 54720
TET1: 52463"
Figure legend: "(C, D) Schematic diagram of the luciferase reporter separated by TET1 intron 8 and 9 or GAPDH intron 3 and 4. DSCR1 reduction increases the activity of this luciferase construct, while DSCR1 overexpression decreases its activity. In contrast, altering DSCR1 levels did not affect luciferase activity of the construct containing GAPDH introns. Values are shown as mean ± SEM, and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 for each condition, *P&lt; 0.05, **P&lt; 0.01. ***P&lt; 0.001."
NCBI gene IDs:
"luciferase: 
GAPDH: 14433
DSCR1: 54720
TET1: 52463"
Figure legend: "(E) TET1 protein expression in the dentate gyrus region of hippocampus of DSCR1 mutants. TET1 is increased in DSCR1 knockout, while it is decreased in DSCR1 transgenic mice. Values are shown as mean ± SEM, and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 3 animal for each condition, *P&lt; 0.05, **P&lt; 0.01."
NCBI gene IDs:
"DSCR1: 54720"
Figure legend: "(A) Genotype of Ts65Dn/DSCR1+/- mouse was confirmed by detecting markers for Ts65Dn and DSCR1 KO"
NCBI gene IDs:
"DSCR1: 54720
Ts65Dn: 21101"
Figure legend: "(B) Expression of the DSCR1 mRNA in Ts65Dn mouse was increased about 2.5 folds compared to that of wild type. However, Ts65Dn/DSCR1+/- mouse showed normal level of DSCR1 mRNA transcript. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N=3 for each condition, *P&lt; 0.05."
NCBI gene IDs:
"DSCR1: 54720
Ts65Dn: 21101"
Figure legend: "As expected, the levels of TET1 are decreased in Ts65Dn mouse, however Ts65Dn/DSCR1+/- mouse restores the TET1 mRNA expression. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. ß-actin was used for normalization. N = 3 (Diploid), 3 (Ts65Dn), 4 (Ts65Dn/DSCR1+/-) mice, *P&lt; 0.05, **P&lt; 0.01."
NCBI gene IDs:
"ß-actin: 11461
DSCR1: 54720
Ts65Dn: 21101
TET1: 52463"
Figure legend: "As expected, the levels of miR-124 are decreased in Ts65Dn mouse, however Ts65Dn/DSCR1+/- mouse restores the miR-124 expression. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. ß-actin was used for normalization. N = 3 (Diploid), 3 (Ts65Dn), 4 (Ts65Dn/DSCR1+/-) mice, *P&lt; 0.05, **P&lt; 0.01."
NCBI gene IDs:
"ß-actin: 11461
miR-124: 70441///268755
DSCR1: 54720
Ts65Dn: 21101"
Figure legend: "(E, F) The number proliferating progenitor neurons identified by BrdU and Ki-67 double staining is restored in the SGZ of Ts65Dn/DSCR1+/- mouse compared to that of Ts65Dn mouse. The white box area is magnified in the lower panels: DAPI (blue), Ki67 (green), and BrdU (red). Arrow heads indicate BrdU and Ki-67 double positive cells in the SGZ. Scale bars: 100 μm in the large image and 10 μm in magnified image. Each hippocampal section was 40 μm in thickness, and total 6 hippocampi were used for analysis. Values are shown as mean ± SEM and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 6 (control), 5 (Ts65Dn), 3 (Ts65Dn/DSCR1+/-) animals, *P&lt; 0.05."
NCBI gene IDs:
"DSCR1: 54720
Ts65Dn: 21101"
Figure legend: "(G-J) Ts65Dn has learning and memory defects, but Ts65Dn/DSCR1+/- mouse clearly rescues learning and memory defects to control levels. Values are shown as mean ± SEM, and tested for statistical significance by one-way ANOVA followed by Bonferroni post hoc test. N = 8 (control), 5 (Ts65Dn), 10 (Ts65Dn/DSCR1+/-) animals, *P&lt; 0.001 (G, H). *P&lt; 0.05 (I), **P&lt; 0.01 (I). (J) Open-field analysis shows no defects in movement of tested animals. Values are shown as mean ± SEM, N = 6 (control), 5 (Ts65Dn), 3 (Ts65Dn/DSCR1+/-) animals."
NCBI gene IDs:
"DSCR1: 54720
Ts65Dn: 21101"
Figure legend: "Binding of recombinant (r)CEACAM1 (CC1)-HIS (10 μg/mL) to (A) a panel of Gram-positive bacteria, namely Enterococcus faecalis, Enterococcus faecium, Group A Streptococcus (GAS), Group B Streptococcus (GBS), Group C Streptococcus (GCS), Group G Streptococcus (GGS), Streptococcus pneumoniae, Staphylococcus aureus, (B) an expanded collection of GBS isolates, with serotype and carriage of bac gene shown. rCC1 binding to live bacteria was quantified by a secondary anti-His-FITC monoclonal antibody (mAb). Mean and standard deviation (SD) values are reported for n = 3 independent experiments. In (B), GBS strains from left to right are A909, BS39, 515, BS22, H36B, BS29, 18RS21, BM110, COH1, SBL3066, NCTC10/84, SB35, SB10, SB20, BS26."
NCBI gene IDs:
"bac: "
Figure legend: "Adhesion of WT, ∆bac and ∆bac + pLZ.bac complemented A909 strains to human CC1- or CC5-expressing CHO transfectants, or empty vector control cells. Mean and SD from n = 5 independent replicates is displayed."
NCBI gene IDs:
"bac: 
CC1: 634
CC5: 1048"
Figure legend: "Binding of rCC1-N to dynabeads (DB) coated with β-IgI3 wild type (WT) or mutants. Mutation of β-IgI3 residues 42, 46 and 53 lead to significant changes in CC1-N binding capacity."
NCBI gene IDs:
"β-IgI3: "
Figure legend: "D The mRNA expression level of METTL3 in sorafenib-sensitive (n=3) and sorafenib-resistant (n=3) human liver tumors. Data information: In all relevant panels, ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "E Expression of METTL3 was significantly down-regulated in advanced stage of liver cancer from TCGA."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "F METTL3 RNA levels between naïve HepG-2 cells (n=3) and sorafenib-resistant HepG-2 cells (n=10). Data information: In all relevant panels, ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "A-B Clonogenic survival of METTL3-knockdown (A) and METTL3-overexpression (B) in normal liver cell line WRL68 cells for 7 days in normoxia condition (21% O2) and quantification of clusters in A-1 and B-1, respectively. Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "C-D The IC50 of METTL3-knockdown SMMC-7721 cells (C) and Bel-7402 cells (D) after treated with sorafenib for 24h under hypoxia condition (1%O2). Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "E Overexpression of wild type METTL3 or catalytic mutant METTL3 in sorafenib-resistant HepG-2 cells."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "F The global RNA m6A level in sorafenib-resistant HepG-2 cells with wild type METTL3-overexpression or catalytic mutant METTL3-overexpression by dot-blotting assay."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "G-I Rescue of shRNA-resistant wild type METTL3 but not catalytic mutant METTL3 sensitized METTL3-knockdown SMMC-7721 cells (G), Bel-7402 cells (H) and sorafenib-resistant HepG-2 cells (I) to sorafenib treatment. The IC50 of the cells was measured after treated with sorafenib for 24h under hypoxia condition (1%O2). Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "J Cell survival assay of sorafenib-resistant HepG-2 cells with wild type METTL3 overexpression or catalytic mutant METTL3 overexpression after treated with sorafenib for 24h under hypoxia condition (1%O2). Scale bar, 1mm."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "K Capillary-like structures in HUVECs treated with the tumor-conditioned medium (TCM) from control HCCs and METTL3-knockdown HCCs cultured under hypoxic conditions for 48 h. Quantification of the number of tubes with image J in K-1. Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "L-M The relative mRNA expression levels of angiogenesis genes were detected in SMMC-7721 cells (L) and Bel-7402 cells (M) with METTL3-knockdown by RT-PCR assay. Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "N The protein levels of VEGF-A in METTL3-knockdown HCCs. O The protein levels of PDGF-B in METTL3-knockdown HCCs. "
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "A Electron micrographs of METTL3-knockdown SMMC-7721 cells under hypoxia for 48 h."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "Protein levels of LC3 I/II in METTL3-knockdown SMMC-7721 (B), Bel-7402 (C) cells under hypoxia condition for 48h."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "F-H Overexpression of shRNA-resistant wild type METTL3 but not catalytic mutant METTL3 rescue autophagy phenotype in METTL3-knockdown SMMC-7721 cells (F), Bel-7402 cells (G) and sorafenib-resistant HepG2 cells (H) by representative immunostaining images of LC3 under hypoxia condition for 48h. Scale bar, 200μm. Quantification of the numbers of GFP-LC3 puncta/cell by Imaris in F-1, G-1 and H-1, respectively. Data information: In all relevant panels, *p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001; Two-tailed t-test. Data are presented as mean ± SD and are representative of 3 independent experiments."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "I-K Protein levels of LC3 I/II in METTL3-knockdown SMMC-7721 cells (I), Bel-7402 cells (J) and HepG-2 cells (K) treated with or without 3-MA under hypoxia condition for 24h."
NCBI gene IDs:
"METTL3: 56339"
Figure legend: "A Gene Set Enrichment Analysis (GSEA) output images of cellular response to stress pathways displaying a correlation of differentially regulated genes in METTL3-knockdown HepG2, SMMC-7721 and Bel-7402 cells with the C5. biological process symbol set. (p &lt; 0.001)."
NCBI gene IDs:
"METTL3: 56339"