EMBO/SourceData huggingface dataset:
Figure legend: "(F) Representative immunohistochemistry (IHC) images detecting FNTA, p-ERK1/2 and p-MEK1/2 in PLKO tumurs as compared to shcircFNTA xenografted tumors. Scale bars: 100 μm (upper panels) and 25 μm (lower panels). (G) Quantification of relative IHC staining intensity revealed that targeting circFNTA led to decreased expression of FNTA, p-ERK1/2 and p-MEK1/2. "
NCBI gene IDs:
"circFNTA: "
Figure legend: "(H) The tumor tail vein injection model was established to observe lung metastasis (N=6). Representative images of lung metastasis foci in gross and microscopic HE examination. Scale bars: 400 μm (upper panels) and 100 μm (lower panels). (I) Knocking down circFNTA significantly decreased metastatic foci numbers as compared to the PLKO group. (J) Targeting circFNTA significantly reduced lung metastatic foci as compared to the PLKO group. "
NCBI gene IDs:
"circFNTA: "
Figure legend: "C. Interaction  between PIF7  and ASF1A/ASF1B  was detected using a semi-in vivo pull-down assay. GST-ASF1A/ASF1B  were purified from E. coli. PIF7-Flash  protein from overexpressing PIF7-Flash seedlings  grown under white light  or 1 h of shade  treatment was detected using an anti-MYC  antibody."
NCBI gene IDs:
"PIF7: 836248"
Figure legend: "F. Interactions  between ASF1A  and PIF7/HIRA  were detected in tobacco leaf  cells. The PIF7-Flash, HIRA-HA, and GFP-ASF1A  (GFP  as control) proteins were co-expressed in tobacco leaf  cells. Anti-GFP Sepharose  beads were used for Co-IP assay."
NCBI gene IDs:
"Flash: 
GFP: 
HA: 
HIRA: 823578
PIF7: 836248
ASF1A: 842992"
Figure legend: "B, Growth  analysis of  Col-0, pif7-1, asf1ab, and hira-1 seedlings  treated with shade. New hypocotyl growth  and real-time growth  rates were recorded at 15-min intervals after shade  treatment."
NCBI gene IDs:
"hira: 823578
pif7: 836248
asf1a: 842992"
Figure legend: "D. Hypocotyl  lengths of Col-0, phyB-9, asf1ab, phyB-9  *   asf1ab,  hira-1, and  phyB-9 * hira-1  grown under white light. The scale bar represents 2 mm. Different letters indicate significant differences (P &lt; 0.01) calculated using one-way ANOVA with Tukey's HSD test. At least 20 seedlings  were used for each treatment or genotype. E. Hypocotyl  lengths of Col-0, pif7-1, asf1ab,  pif7-1 *  asf1ab, hira-1, and  pif7-1 * hira-1 under white light and shade  conditions. The scale bar represents 2 mm. Different letters indicate significant differences (P &lt; 0.01) calculated using one-way ANOVA with Tukey's HSD test. At least 20 seedlings were used for each treatment or genotype."
NCBI gene IDs:
"hira: 823578
phyB: 816394
pif7: 836248
asf1a: 842992"
Figure legend: "B. Boxplot displays the fold changes in shade-induced genes in Col-0, pif7-1, asf1ab, and hira-1 by comparing the transcript levels between  white light  and shade  conditions. Different letters indicate significant differences (P &lt; 0.05) as determined by one-way ANOVA."
NCBI gene IDs:
"hira: 823578
pif7: 836248
asf1a: 842992"
Figure legend: "D. Integrative genomics viewer (IGV) screenshots showing the distribution of GFP-ASF1A  enrichment at the ATHB2, PIN7, CSLC4, BIM1, ARL, and NPY8  loci."
NCBI gene IDs:
"NPY8: 
ARL: 819014
BIM1: 830708
CSLC4: 822444
HB2: 820216
PIN7: 838916"
Figure legend: "G. ChIP-PCR  analysis of ASF1  enrichment using anti-ASF1A  antibodies at the ATHB2, PIN7, CSLC4, BIM1, ARL, and NPY8  loci. Col-0 and pif7-1 seedlings  were grown under continuous  white light or transferred to the shade  for 1 h. Top panels show a schematic representation of the gene structures. The bottom panels represent the effects of shade  on ASF1A  enrichment. Shade-increased enrichment of ASF1A  was calculated as input% SH  minus input% WL. The difference at the P2 locus in Col-0 was normalized to one for each gene. Different letters indicate statistically significant differences (P &lt; 0.05) by one-way ANOVA with Tukey's HSD test. The data shown are the mean ± SDs (n = 3, where n refers to technical replicates)."
NCBI gene IDs:
"NPY8: 
ARL: 819014
BIM1: 830708
CSLC4: 822444
HB2: 820216
pif7: 836248
PIN7: 838916"
Figure legend: "G. IGV screenshots show the distribution of H3.3  and H3.1  enrichment levels at the ATHB2, PIN7, CSLC4, BIM1, ARL, and NPY8  loci."
NCBI gene IDs:
"NPY8: 
ARL: 819014
BIM1: 830708
CSLC4: 822444
HB2: 820216
PIN7: 838916"
Figure legend: "H. Hypocotyl  lengths of Col-0, pif7-1, and h3.3kd under white light and shade  conditions. The scale bar represents 2 mm. Different letters indicate significant differences (P &lt; 0.01) calculated using one-way ANOVA with Tukey's HSD test. At least 20 seedlings  were used for each treatment or genotype."
NCBI gene IDs:
"h3.3: 830164
pif7: 836248"
Figure legend: "I. Relative expression  levels of ATHB2, PIN7, CSLC4, BIM1, ARL, and NPY8  in Col-0, pif7-1, and  h3.3kd seedlings  grown under white light  or shade  conditions. The seedlings  were grown under  white light  for 6 d, and then maintained under  white light, or transferred to shade  for 1 h. Different letters indicate statistically significant differences (P &lt; 0.05) by one-way ANOVA with Tukey's HSD test. The data shown are the mean ± SDs (n = 3, n refers to biological replicates)."
NCBI gene IDs:
"NPY8: 
ARL: 819014
h3.3: 830164
BIM1: 830708
CSLC4: 822444
HB2: 820216
pif7: 836248
PIN7: 838916"
Figure legend: "E. ChIP-PCR  analysis of HTR5  (H3.3) levels at the ATHB2, PIN7, CSLC4, BIM1, ARL, and NPY8  loci. Top panels show a schematic representation of the gene structures. The bottom panels represent the effects of shade  on H3.3 enrichment. Shade-increased enrichment of H3.3  was calculated as the input% SH  minus input% WL. The difference at the P2 locus in Col-0 was normalized to one for each gene. Different letters indicate statistically significant differences (P &lt; 0.05) by one-way ANOVA with Tukey's HSD test. The data shown are the means ± SDs (n = 3, where n refers to biological replicates)."
NCBI gene IDs:
"NPY8: 
ARL: 819014
BIM1: 830708
CSLC4: 822444
HB2: 820216
PIN7: 838916"
Figure legend: "(E) Top, Representative images of live HeLa  cells in cytokinesis transiently transfected with GFP-CENP-E2605-2701 and mutants (green), incubated with SiR-tubulin  (red). Scale bar, 10μm. Bottom, linescans showing the fluorescence intensity average and standard error of the mean (SEM) for the GFP-CENP-E2605-2701 and mutants and tubulin  across the cell midbody. For GFP-CENP-E2605-2701, n is the number of cells. n=11 and for the GFP-CENP-E2605-2701 mutants FDN2661LTT, YF2660AA, FF2644AA, n= 15, 16 and 25 respectively. Biological independent replicates were respectively 2, 3, 2 and 1."
NCBI gene IDs:
"GFP: 
CENP-E: 1062"
Figure legend: "(B) Representative images of live HeLa  cells in mitosis transiently transfected with either GFP-Kif4A1133-1165 or GFP-Kif4A1133-1232 incubated with SiR-Tubulin. Scale bar, 10μm. Quantification of cells with GFP-Kif4A1133-1165 (n=12) or GFP-Kif4A1133-1232 (n=13) localization  to the  central spindle. Data represented from 2 independent experiments."
NCBI gene IDs:
"GFP: 
Kif4A: 24137"
Figure legend: "(D) Live-cell imaging of  metaphase spindles in HeLa  cells transiently transfected wild-type, phosphomimetic and non-phosphorylatable mutants of GFP-CENP-E2605-2701 (monomeric) and GFP-GST-CENP-E2605-2701 (dimeric) and stained  for tubulin  using SiR-Tubulin. The fraction of cells localizing  to the overlapping microtubules  is represented as a percentage. Scalebar, 10 μm."
NCBI gene IDs:
"GFP: 
GST: 
CENP-E: 1062"
Figure legend: "(G) Bar graph showing mean and standard error for GFP  fluorescence intensity at peak fluorescence for GFP-GST-CENP-E2605-2701 and mutants at 9.7μm, quantified in F. Asterisks indicate ordinary One-way Anova test significance value. ****P&lt;0.0001."
NCBI gene IDs:
"GFP: 
GST: 
CENP-E: 1062"
Figure legend: "(A) Representative immunofluorescence  images of HeLa  cells in  late anaphase and telophase, expressing GFP-PRC1-WT or -MEE and depleted for endogenous PRC1  using a PRC1  siRNA and a PRC1  sgRNA after doxycycline-induced Cas 9 expression. Microtubules, GFP  and CENP-E are in white, green and red respectively. DNA  is in blue. (B) Graph showing the percentage of cells with a PRC1-localized  at the site of abscission for cells treated in (A). Mean and standard deviation are presented, n= 156 and 141 for cells expressing PRC1-WT and -MEE respectively. Data from 4 biological replicates and 1 technical replicate. Asterisks indicate a T-test significance value. ****P&lt;0.0001. (C) Graph showing the percentage of cells with CENP-E localized  at the abscission site for cells treated in (A), mean and standard deviation are presented, n= 58 and 66 for cells expressing PRC1-WT and -MEE respectively. Data from 2 biological replicates. p-value (p=0.11) calculated for an unpaired T-test."
NCBI gene IDs:
"GFP: 
Cas 9: 69900935
PRC1: 9055"
Figure legend: "(G) Representative live-cell images of HeLa  cells expressing GFP-PRC1-WT or -MEE and depleted for endogenous PRC1  using a PRC1  siRNA and a PRC1  sgRNA after doxycycline-induced Cas 9  expression. Microtubules  (SiR-Tubulin) and DNA  (SPY650) are shown in red. The cell membrane (CellMask) is highlighted in white and GFP-PRC1  is in green. Scalebar, 10 μm."
NCBI gene IDs:
"GFP: 
Cas 9: 69900935
PRC1: 9055"
Figure legend: "(J) Representative immunofluorescence  images of cells expressing GFP-PRC1-WT or -MEE after 72 hours siRNA depletion and doxycycline-induced knockout of endogenous PRC1. DNA, microtubules  and GFP-PRC1/GFP-PRC1-MEE are in blue, red and green respectively. Scalebar, 10μm."
NCBI gene IDs:
"GFP: 
PRC1: 9055"
Figure legend: "c, Log normalised expression of markers for epithelial (EPCAM), mature luminal epithelial (ESR1), myoepithelial (KRT5, KRT14 and ACTA2) and proliferating cancer cells (MKI67)."
NCBI gene IDs:
"ACTA2: 59
EPCAM: 4072
ESR1: 2099
KRT14: 3861
KRT5: 3852
MKI67: 4288"
Figure legend: "e, Log normalised expression of markers for fibroblasts (PDGFRB, THY1, COL1A1, ITGB1 and S100A4), endothelial cells (PECAM1), T-cells (CD3D), CD8 T cells (CD8A), T-regulatory cells (FOXP3), B-cells (MS4A1), myeloid cells (CD68) and plasma cells (JCHAIN)."
NCBI gene IDs:
"CD3D: 915
CD68: 968
CD8A: 925
COL1A1: 1277
FOXP3: 50943
ITGB1: 3688
JCHAIN: 3512
MS4A1: 931
PDGFRB: 5159
PECAM1: 5175
S100A4: 6275
THY1: 7070"
Figure legend: "b, Candidate transcriptional drivers of each CAF and PVL subset. Violin plots showing the log normalised gene expression (left) of the TF and its respective AUC regulon activity (right). TFs ZEB1 and FOXP1 enriched in myofibroblast-like CAFs, EGR2 and TCF7L2 enriched in inflammatory-like CAFs, MEF2C enriched in PVL cells and NR2F2 enriched in immature-PVL cells."
NCBI gene IDs:
"EGR2: 1959
FOXP1: 27086
MEF2C: 4208
NR2F2: 7026
TCF7L2: 6934
ZEB1: 6935"
Figure legend: "q-RT-PCR reveals that miR-181a/b silencing leads to upregulation of miR-181a/b predicted targets in SH-SY5Y cells. N=3 independent experiments."
NCBI gene IDs:
"miR-181a: 406995"
Figure legend: "miR-181-mimic transfection specifically inhibits Luciferase activity of constructs containing WT 3'-UTR predicted target sequences. Point mutations (mut) in miR-181a/b binding sites abolish Luciferase repression in all cases apart from PPARGC1A. Data are normalized to negative mimic transfection (dashed line). N=6 independent experiments."
NCBI gene IDs:
"miR-181a: 406995
miR-181: 406955
PPARGC1A: 10891"
Figure legend: "q-RT-PCR reveals upregulation of miR-181a/b targets in the eyes of miR-181a/b-1-/- vs. miR-181a/b-1+/+ animals. n≥5 animals/genotype."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "miR-181a/b-1-/- mice show increased mtDNA content vs. miR-181a/b-1+/+ mice as measured by q-PCR. N=4 animals/genotype."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "WB analysis (left panel) reveals increased levels of mitochondrial proteins in the eyes of miR-181a/b-1-/- (-/-) vs. miR-181a/b-1+/+ (+/+) mice (quantified in the right panel). Data are normalized to either p115 or Gapdh. N≥3 animals/genotype. Please note that all compared bands from +/+ and -/- samples are from the same blots which were cropped and shown split for the sake of data presentation clarity."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "Cell death analysis shows that miR-181a/b silencing protects SH-SY5Y cells from FCCP treatment. N≥7 independent experiments."
NCBI gene IDs:
"miR-181a: 406995"
Figure legend: "TUNEL assays on eye and brain sections of stage (st)30 medakafish control and MO-injected embryos reveal a decrease in the number of apoptotic cells in both hccs-MO/miR-181a/b-MO- and cox7b-MO/miR-181a/b-MO-injected compared to hccs-MO- and cox7b-MO-injected embryos. Scale bars are 20μm."
NCBI gene IDs:
"hccs: 101166931
cox7b: 101167565
miR-181a: 102466480"
Figure legend: "Caspase assays show restored levels of caspase-3 and caspase-9 activities in hccs-MO/miR-181a/b-MO- and cox7b-MO/miR-181a/b-MO-injected embryos with respect to hccs-MO- and cox7b-MO-injected embryos. n≥5 embryos for each model."
NCBI gene IDs:
"hccs: 101166931
cox7b: 101167565
miR-181a: 102466480"
Figure legend: "Representative images of st30 medaka embryos injected with hccs-MO (B) alone or co-injected with miR-181a/b-MOs Co-injection of miR-181a/b-MOs rescues microphthalmia and microcephaly in both hccs-MO embryos. hccs-MO/miR-181a/b-MO- b-MO-injected embryos were treated with Baf-A1, PD98059 or HA14-1. Baf-A1 treatment counteracts the protective effect of miR-181a/b downregulation in hccs-MO/miR-181a/b-MO embryos PD98059 treatment does not interfere with the modulation of the MLS phenotype mediated by miR-181a/b downregulation HA14-1 treatment counteracts the effect of miR-181a/b downregulation in the hccs-MO/miR-181a/b-MO model Scale bars are 100μm."
NCBI gene IDs:
"hccs: 101166931
miR-181a: 102466480"
Figure legend: "Representative images of st30 medaka embryos injected with cox7b-MO (H) alone or co-injected with miR-181a/b-MOs Co-injection of miR-181a/b-MOs rescues microphthalmia and microcephaly in cox7b-MO embryos. cox7b-MO/miR-181a/b-MO-injected embryos were treated with Baf-A1, PD98059 or HA14-1. Baf-A1 treatment counteracts the protective effect of miR-181a/b downregulation in cox7b-MO/miR-181a/b-MO embryos PD98059 treatment does not interfere with the modulation of the MLS phenotype mediated by miR-181a/b downregulation Scale bars are 100μm."
NCBI gene IDs:
"cox7b: 101167565
miR-181a: 102466480"
Figure legend: "Percentage of embryos with or without MLS phenotype in the different conditions in hccs-MO embryos. N≥300 embryos/conditions."
NCBI gene IDs:
"hccs: 101166931"
Figure legend: "Percentage of embryos with or without MLS phenotype in the different conditions in cox7b-MO (N) embryos. N≥300 embryos/conditions."
NCBI gene IDs:
"cox7b: 101167565"
Figure legend: "Immunofluorescence analysis with anti-NeuN antibody in the retina of miR-181a/b-1+/+ and miR-181a/b-1-/- mice intravitreally injected with Rotenone or DMSO. RGCs are preserved in miR-181a/b-1-/- rotenone-injected mice with respect to controls at both one and two weeks after injection. Scale bars are 50μm."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "NADH dehydrogenase histochemical reaction on retinal sections of miR-181a/b-1+/+ and miR-181a/b-1-/- mice intravitreally injected with Rotenone or DMSO. At one week post-injection, NADH dehydrogenase activity is lost in RGCs (GCL, areas within dashed lines) of miR-181a/b-1+/+ Rotenone-injected eyes, while it is preserved in those of miR-181a/b-1-/- Rotenone-injected eyes. GCL, Ganglion Cells Layer ONL; INL, Inner Nuclear Layer; Outer Nuclear Layer. Scale bars are 50μm."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "Immunofluorescence analysis, with an anti-CoxIV antibody, in the retina of miR-181a/b-1+/+ and miR-181a/b-1-/- mice, injected with either Rotenone or DMSO, showed preserved mitochondria in miR-181a/b-1-/- rotenone-injected eyes at one-week post-injection. Dashed boxes indicate the area of magnifications shown in right panels. Scale bars are 10μm."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "Graphical representation of the results of the optokinetic tracking assays reported as fold change of cycles/degree. Visual acuity is preserved in miR-181a/b-1-/- rotenone-injected mice with respect to controls at both one and two weeks after injection. N=10."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "Immunofluorescence analysis with anti-NeuN antibody in the retina of WT, Ndufs4-/-, Ndufs4-/-/miR-181a/b-1-/- and miR-181a/b-1-/- mice. RGCs are preserved in Ndufs4-/-/miR-181a/b-1-/- with respect to Ndufs4-/- mice. Scale bars are 50μm."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "Graphical representation of the results of the optokinetic tracking assays reported as fold change of cycles/degree. Visual acuity is preserved in Ndufs4-/-/miR-181a/b-1-/- with respect to Ndufs4-/- mice. N≥8."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "Electroretinographic analysis reveals amelioration of b-wave patterns in Ndufs4-/-/miR-181a/b-1-/- with respect to Ndufs4-/-mice. N≥5."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "Electron microscopy analysis shows amelioration of mitochondria morphology and increase of mitochondrial number in Ndufs4-/-/miR-181a/b-1-/- vs. Ndufs4-/- mice. Scale bars are 1μm. The quantitative increase of mitochondria is reported in C as number of mitochondria/RGC, at p30 and p55. N≥2 animals/genotype."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "Ndufs4-/-/miR-181a/b-1-/- mice show increased mtDNA content vs. Ndufs4-/- mice as measured by q-PCR. N≥4 animals/genotype."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "Biochemical activity of MRC complexes I, II and IV normalized by the percentage of citrate synthase (CS) activity in Ndufs4-/-/miR-181a/b-1-/- vs. Ndufs4-/- mice. N≥3 animals/genotype. p‐values were calculated by two tailed Student's t-test; Error bars are SEM."
NCBI gene IDs:
"miR-181a: 735252
Ndufs4: 17993"
Figure legend: "(A) Graph derived from two published data available in the PubMed GEO database (GSE6919 and GSE35988). The box charts depict the relative expression of ESM1 in benign, primary, and metastatic prostate cancer patients. The centre line donates the median value while the box contains the 25th to 75th percentiles of dataset. The whiskers mark the 5th and 95th percentiles, and values beyond these upper and lower bounds are considered outliers."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(B) Relative ESM1 mRNA expression and ESM1 protein expression in two sets of human metastatic PCa cell lines. P: parental cells, M: metastatic cells."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(C) Cell invasion assays of 22Rv1-M and PC3-M cells transfected with either Scramble-shRNA or ESM1-shRNA. The data are shown as the relative fold change of invasive cells compared with the shScramble group. Scale bar: 100 μm."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(D) Representative images of lungs, livers, intestines, and kidneys from mice 8 weeks after orthotopical injection of 22Rv1-M cells transfected with either Scramble-shRNA or ESM1-shRNA. Scale bar: 0.5 cm. Tumors were removed and weighed. Quantitative summary of tumor luminescence in different metastases measured in photons/second are shown."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(D) Elicitation of ESM1 overexpression using HA-tag antibody results in detectable ESM1 expression in the nucleus and cytoplasm of 22Rv1-P cells. Lamin A/C and α-tubulin were used as loading control in the nucleus and cytoplasm, respectively."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(F) Left, representative images of 22Rv1-P cells in the transwell invasion assay. Right, statistic data of invasive ability of ESM1 expressing cells. Scale bar: 100 μm."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(G) Left, representative bioluminescence images of lung metastasis in mice after tail vein injection of 22Rv1-Luc cells with different ESM1 expression patterns. Scale bar: 0.5 cm. Right, quantification of the lung bioluminescence by photons/ seconds."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(C) 22Rv1-P cells stably expressing ESM1 were treated with docetaxel at the indicated concentrations for 48 h. **"
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(D) Cells with or without ESM1-NLS overexpression were subcutaneously injected (10, 100, 1000 cells per mouse) into NOD/SCID mice. Tumor formation ability and tumor weight were analyzed. Scale bar: 1 cm. Bars are the mean ± SD of indicated (n) independent experiments."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(E) Flow cytometry analysis of the ratio of CD44+ and CD133+ cells in the indicated cells with different subcellular localization types of ESM1 overexpression were performed."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(F) qRT-PCR analysis of the mRNA levels of the indicated genes in cells with different types of ESM1 overexpression. Differences in mRNA levels compared with vector cells are shown as fold changes presented as the mean ± SD of three independent experiments. *"
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(E) The transcriptional activity of β-catenin in 22Rv1-P cells with different patterns of ESM1 overexpression. Bars are the mean ± SD of three independent experiments."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(F) Manipulating ESM1 expression impacts transcriptional activity of NFκB. Bars are the mean ± SD of three independent experiments."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(C) Immunoblotting analysis of 22Rv1-P cells with different ESM1 overexpression in the nucleus and cytoplasm were performed with the antibodies indicated."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(D) Suppression of β-catenin or NFκB in 22Rv1-P cells stably expressing ESM1-NLS were treated with docetaxel at the indicated concentrations for 48 h. ** P &lt; 0.01 when compared to vector-shScramble cells by two-tailed Student's t test and error bars represent the standard deviation of three independent experiments."
NCBI gene IDs:
"β-catenin: 1499
ESM1: 11082
NFκB: 4790"
Figure legend: "(E) Tumor spheres formed by 22Rv1-P cells stably expressing ESM1-NLS with either β-catenin or NFκB shRNAs. Scale bar: 100 μm. Bars are the mean ± SD of three independent experiments. ## P&lt; 0.01 when compared to vector-shScramble cells, ** P&lt; 0.01 when compared to ESM1-NLS-shScramble cells by two-tailed Student's t test."
NCBI gene IDs:
"β-catenin: 1499
ESM1: 11082
NFκB: 4790"
Figure legend: "(F) Representative images and quantitative data comparing cell invasion of the indicated cells. Scale bar: 100 μm. Bars are the mean ± SD of three independent experiments. ## P&lt; 0.01 when compared to vector-shScramble cells, ** P&lt; 0.01 when compared to ESM1-NLS-shScramble cells by two-tailed Student's t test."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(A) Nuclear and cytosolic extracts of 22Rv1-M cells transfected with either shScramble or shCTNNB1 were prepared and the levels of indicated proteins were detected by immunoblotting."
NCBI gene IDs:
"CTNNB1: 1499"
Figure legend: "(E) HEK293T cells were co-transfected with the indicated β-catenin-Flag and ESM1-HA constructs. Cell extracts were immunoprecipitated with Flag-M2 agarose beads. Light chain was labeled as l.c."
NCBI gene IDs:
"Flag: 
HA: 
β-catenin: 1499
ESM1: 11082"
Figure legend: "(F) HEK293T cells were transfected with ESM1-HA constructs and pull-down was carried out with different purified GST-ARM fragments."
NCBI gene IDs:
"GST: 
HA: 
ESM1: 11082"
Figure legend: "(G) HEK293T cells were transfected with the indicated ESM1-GFP constructs. Cell extracts were pulled down with purified GST-ARM."
NCBI gene IDs:
"GFP: 
GST: 
ESM1: 11082"
Figure legend: "(B) HEK293T cells were transfected with the indicated ESM1 point mutant constructs. Cell extracts were pulled down with purified GST-ARM."
NCBI gene IDs:
"ESM1: 11082"
Figure legend: "(C) Cell extracts of 22Rv1-M transfected with either Scramble-shRNA or ESM1-shRNA were immunoprecipitated with an β-catenin antibody and blotted with anti-TCF4 and anti-BCL9 antibody."
NCBI gene IDs:
"ESM1: 11082"