EMBO/SourceData huggingface dataset:
Figure legend: "D MICAL2PV mutants containing the MO domain are active in depolymerizing F-actin. Following transfection of plasmids expressing different HA-MICAL2PV mutants (#1, #2, or #4) into shM2 cells, confocal microscopy was performed following staining with phalloidin (red) and anti-HA antibody (green).  F-actin signals were reduced in cells expressing different mutants (HA-positive green cells marked by dashed lines). Scale bar: 20 μm. Data information: nuclei were stained with Hoechst 33342 (blue)."
NCBI gene IDs:
"HA: 
M2: 9645
MICAL2PV: 9645"
Figure legend: "G, H The  MICAL2PV mutant containing an inactive MO domain does not inhibited TNT formation. Stable shM2 H1299 (G) or A549 (H) cells were transfected with plasmids expressing the MICAL2PV  mutant in which the MO domain was inactivated by mutations in the FAD binding site (MOmut: amino acid residues 93-98 GxGxxG mutated to WxWxxW) or the control vector (Ctr). Following transfection, Western blotting and quantification of TNT formation were carried out as in panel E. 10 images were captured for quantification in each group. Three independent experiments were performed. Data information: Data were analyzed using a one-way ANOVA with Bonferroni post-test and shown as mean ± SEM; * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001, ns, not significant."
NCBI gene IDs:
"M2: 9645
MICAL2PV: 9645"
Figure legend: "Live cell microscopy  of  U-2 OS-TetO cells expressing rTetR-GFP   (ctrl), or rTetR-GFP-Kin14VIb  (Kin14VIb) (B) Representative stills showing the most frequently observed metaphase localization  and daughter nuclei  distribution of the duplicated TetO locus (white arrowheads). Chromatin  was visualized through expression  of H2B-mCherry, GFP  depicts the TetO locus. Due to the presence of a predicted NES sequence in Kin14VIb, the TetO locus only becomes visible at NEB   in Kin14VIb-expressing cells. Time  = h:min, scale bar = 5 µm. Schemes illustrating the metaphase localization  of the TetO locus and its subsequent distribution over daughter nuclei  are shown on the right."
NCBI gene IDs:
"GFP: 
Kin14VIb: 112281002"
Figure legend: "(C) Quantification of the fraction of ctrl or Kin14VIb-expressing cells with the TetO locus observed outside the metaphase  plate (TetO out). (D) Deconstruction of (C). Fraction of cells with the indicated orientation of the duplicated TetO locus and TetO chromosome. Data information: Bars represent the mean of 2 biological replicates, while dark- and light-colored dots represent the values of each experiment. (C, D): N ≥ 29 cells per condition, per experiment, ****P&lt;0.0001; Fisher's exact test."
NCBI gene IDs:
"Kin14VIb: 112281002"
Figure legend: "E. Quantification of the number of cells with Mad1-positive KTs  nearby the TetO locus. In case the KTs  of the TetO chromosome  were aligned, we scored whether KTs  in the vicinity of the TetO locus were Mad1+. In case the KTs of the TetO chromosome  could be distinguished in rTetR-GFP-Kin14VIb-expressing cells (with either orthogonal or parallel oriented KTs), we scored if at least one of the KTs  was Mad1+. Data information: Bars represent the mean of 2 biological replicates, while dark- and light-colored dots represent the values of each experiment. (E): N ≥27 cells per condition, per experiment, (ns) not significant; Fisher's exact test."
NCBI gene IDs:
"GFP: 
Kin14VIb: 112281002"
Figure legend: "A-D (A, C) Frequencies of the different types of segregation errors observed during anaphase  (A) and late anaphase/telophase  (C). Schemes illustrating the observed types of anaphase  errors are shown on the right of (A). Cells within the main TetO locus distribution categories in control (1-1), and Kin14VIb  (2-0) conditions were scored (see also Fig EV1A). The majority of Kin14VIb-expressing anaphases  display at least one stretched arm (CENP-C in line with TetO locus at the pole) or a bridge in line with the TetO locus at the pole  (\"stretched arm or bridge in line\"), some of them with additional bridges (+). (B, D) IF  images for  CENP-C, GFP, H3S10ph of  U-2 OS TetO cells in anaphase  (B) and late anaphase  or telophase  (D), expressing either rTetR-GFP  or rTetR-GFP-Kin14VIb. Images (maximum intensity projections) represent the most frequently observed anaphase  and telophase  errors in cells with either a 1-1 (majority of events in ctrl), or 2-0 (majority of events in Kin14VIb) TetO locus distribution. Scale bar = 5 µm. Yellow arrowheads indicate TetO locus, red arrowheads CENP-C. Data information: (A, C): Means ± S.E.M. of 3 biological replicates are shown. Dots represent the values of each experiment. N= 41-89 cells per condition, per experiment ****P&lt;0.0001, (ns) not significant; Fisher's exact test. (B, D): for optimal visibility of the TetO foci, the brightness and contrast of all channels were linearly adjusted for individual cells."
NCBI gene IDs:
"GFP: 
Kin14VIb: 112281002"
Figure legend: "A. Representative images of U-2 OS TetO anaphases/telophases  expressing rTetR-GFP  or rTetR-GFP-Kin14VIb  with FISH-marked centromeres  of chromosome 1 (chr1-CEN). White and yellow arrowheads: chr1-CEN  segregated into the main masses of segregating chromosomes; red arrowheads: chr1-CEN FISH  signal fragmented or splitted in a bridge  (bridging); chr1-CEN  lagging  between the two main masses of segregating chromosomes  (lagging); chr1-CEN  late arrival (late-equal: one CEN  focus in but near the edge of a separated chromosome  mass and connected to a bridge). For clarity, the maximum intensity projection of a subset of z stacks (6-12/64) is shown. Scale bar = 5 µm. B. Frequency of the different types of errors observed in A. The mean (bars) and individual values (dots) of 2 independent experiments are shown. Data information: In (A), for optimal visibility of the chr1-CEN FISH  foci, the brightness and contrast were linearly adjusted for individual cells. (B): n ≥ 45 cells per condition, per experiment. Bars represent the mean of 2 biological replicates, while dark- and light-colored dots represent the values of each experiment. * P&lt;0.05, (ns) not significant. Fisher's exact test."
NCBI gene IDs:
"GFP: 
Kin14VIb: 112281002"
Figure legend: "C. IF  for Cas9  and CENP-C of metaphases  of RPE1-dCas9-Kin14VIb  cells transduced with Chr1-telo  sgRNA in the presence or absence of 500 nM rapalog. The Chr1-telo  loci (Cas9  foci, white arrowheads) are aligned on the metaphase  plate in control condition (- rapalog), while the loci are facing either a single or opposite poles  after rapalog  addition to induce Kin14VIb binding  to dCas9. Scale bar = 5 µm. For clarity, the maximum intensity projection of a subset of z stacks (18-50/100) is shown. Data information: (C for optimal visibility of the Chr1-telo  foci, the brightness and contrast of all channels were linearly adjusted for individual cells."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "D,  Live cell microscopy   of asynchronously growing RPE1-dCas9-Kin14VIb  cells transduced with Chr1-telo  sgRNA in the presence or absence of 500 nM rapalog  to induce kinesin binding  to the subtelomeric  locus. (D) Stills showing the most frequently observed metaphase localization  and daughter cell  distribution (white arrowheads) of the duplicated Chr1-telo  loci. SiR-DNA  was used to visualize the DNA, GFP  depicts the Chr1-telo  loci. Time  (h:min). Scale bar = 5 µm. Note that in the upper panel, the cell was already in mitosis when imaging started (t =0:00). Data information: (D, Cells were derived from three independent imaging experiments. N = 23 cells (- rapalog), n = 41 cells (+ rapalog). Sp = same pole, op = opposite pole, ss = same sisters. D): for optimal visibility of the Chr1-telo  foci, the brightness and contrast of all channels were linearly adjusted for individual cells."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "B,  Live cell microscopy  of asynchronously growing RPE1-dCas9-Kin14VIb  cells transduced with Chr9-cen  sgRNA and plus/minus rapalog  to induce kinesin binding  to the pericentromeric  locus (Movies S4-S8). (B) Stills of the most frequently observed metaphase  orientations and daughter cell distributions of the duplicated Chr9-cen  loci (white arrowheads) are shown.  SiR-DNA was used to visualize the DNA, GFP  depicts the Chr9-cen  loci. Time  (h:min). Scale bar = 5 µm. Data information: Cells were derived from two independent imaging experiments.. In (B), for optimal visibility of the Chr9-cen  foci, the brightness and contrast of all channels were linearly adjusted for individual cells. See Movies EV4- S8 for equally adjusted corresponding examples."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "D. Time  between  nuclear envelop break down (NEB) and anaphase  onset in the presence (Kin14VIb  bound to dCas9) or absence (Kin14VIb  expressed, but not bound to dCas9) of rapalog. Data information: Cells were derived from two independent imaging  experiments.. (D): Mean and S.D. are shown."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "A. Representative IF  images of RPE1-dCas9-Kin14VIb  cells, showing the localization  of the Chr9-cen  loci in metaphase  in the absence or presence or rapalog. Magnifications of the white boxed regions (each region showing one Chr9-cen  locus) are shown in the corners (scale bars = 2 µm). The fraction of cells with Chr9  mis-aligned and CENP-C near the locus (Cas9) is indicated below the image. For clarity, the maximum intensity projection of a subset of z stacks in which the Chr9-cen  loci are in focus (2-10/100) is shown. B. Frequency of the observed metaphase localizations  of the Chr9-cen  loci as shown in (A). Data information: In all cases, bars represent the mean of 2 independent experiments, while dark- and light-colored dots represent the values of each experiment. (B): **** P&lt;0.0001, Fisher's exact test. N ≥ 31 cells per condition, per experiment. (A, For optimal visibility of the Chr9-cen  foci the brightness and contrast of all channels were linearly adjusted for individual cells."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "C. Representative IF   images of RPE1-dCas9-Kin14VIb  cells, showing the segregation and distribution of Chr9-cen  loci in anaphase  (white arrowheads). Red arrowheads indicate H3S10ph-positive chromatin bridges. For clarity, the maximum intensity projection of a subset of z stacks in which the chr9-cen  loci are in focus (30-62/100) is shown. Scale bar = 2 µm. Data information: C): For optimal visibility of the Chr9-cen   foci H3S10ph-positive  chromatin bridges, the brightness and contrast of all channels were linearly adjusted for individual cells."
NCBI gene IDs:
"Cas9: 69900935
Kin14VIb: 112281002"
Figure legend: "(B) The region of FYCO1 between aa 1,276 and 1,294 is essential and sufficient for the interaction with LC3B. GST or GST-LC3B were incubated with [S35]methionine-labeled deletion mutants of FYCO1 and processed as in Fig. 1 C."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(C) LIR is essential for colocalization of FYCO1 with LC3B in HeLa cells. HeLa cells expressing the indicated constructs were imaged by confocal microscopy. Insets show an enlarged field of interest."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(D) Both N- and C-terminal domains of LC3B are needed for efficient interaction with FYCO1. GST, GST-LC3B, or its deletion mutants were incubated with [S35]methionine-labeled myc-FYCO1 and processed as in Fig. 1 C. ARG, autoradiography; CB, Coomassie blue."
NCBI gene IDs:
"LC3B: 81631"
Figure legend: "(C) FYCO1 can self-interact in a CC-dependent manner. Full-length GFP-FYCO1 was cotranscribed and cotranslated with the indicated deletion mutants of myc-tagged FYCO1 in rabbit reticulocyte lysate. S35-labeled FYCO1 complexes were immunoprecipitated with anti-myc antibody, separated by SDS-PAGE, and visualized by autoradiography. IP, immunoprecipitation."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(B) FYCO1990-1,233 is the smallest FYVE domain-containing deletion mutant of FYCO1 that can efficiently interact with the full-length FYCO1. Full-length myc-FYCO1 was cotranscribed and cotranslated with the indicated deletion mutants of GFP-tagged FYCO1 in rabbitreticulocyte lysate. S35-labeled FYCO1 complexes were immunoprecipitated with anti-SDS-PAGE antibody, separated by SDS-PAGE, and visualized by autoradiography. IP, immunoprecipitation."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(A) Rab7Q67L recruits FYCO11,038-1,233 and FYCO11,091-1,233 but not FYCO11,156-1,233 to the cytosolic vesicles. HeLa cells transfected with GFP-FYCO11,038-1,233 (top), GFP-FYCO11,091-1,233 (bottom), or GFP-FYCO11,156-1,233 (middle right) with or without mCherry-Rab7Q67L were imaged 24 h after transfection. The graph shows the relative number of cells ± SD with GFP-FYCO11,038-1,233 recruited to intracellular membranes without and with cotransfected mCherry-Rab7Q67L."
NCBI gene IDs:
"FYCO1: 79443
Rab7: 338382///7879"
Figure legend: "(B) Rab7Q67L recruits the 2xFYVEFYCO1 construct containing a short CC region in front of the first FYVE domain (FYCO11,091-1,233+1,156-1,233) but not a 2xFYVE alone to the perinuclear vesicles. HeLa cells transfected with GFP-FYCO11,091-1,233+1,156-1,233 or GFP-FYCO11,156-1,233+1,156-1,233 with or without mCherry-Rab7Q67L were imaged 24 h after transfection."
NCBI gene IDs:
"FYCO1: 79443
Rab7: 338382///7879"
Figure legend: "(D) Leucine-1151 and tryptophan-1152 are important for the recruitment of FYCO1 to Rab7-containing vesicles. HeLa cells transfected with GFP-FYCO1990-1,233L1151A/W1152A with or without mCherry-Rab7Q67L were imaged 24 h after transfection. (B and D) Insets show an enlarged field of interest."
NCBI gene IDs:
"FYCO1: 79443
Rab7: 338382///7879"
Figure legend: "(D) Full-length FYCO1 but not the FYCO1 lacking aa 555-1,136 can redistribute Rab7 and ORP1L to the cell periphery. HeLa cells were transfected with the indicated constructs and stained with DRAQ5."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(E) The region of FYCO1 between aa 675 and 771 is essential for the MT plus end translocation of FYCO1-decorated structures. HeLa cells were imaged 24 h after transfection with the indicated constructs."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(B) Representative images of HeLa cells with or without the perinuclear clusters of GFP-LC3-positive vesicles (right) and quantification of the number of cells with this phenotype 48 h after transient transfection with siRNAs against FYCO1 (left). Error bars represent SDs based on three independent experiments."
NCBI gene IDs:
"FYCO1: 79443"
Figure legend: "(A) Raji-Env cells were subcloned to obtain cell lines expressing increasing amounts of Env. Env surface levels were determined by the binding of 10-1074 and measured by flow cytometry. One representative experiment is shown."
NCBI gene IDs:
"Env: 155971"
Figure legend: "(D) HIV- or HIV∆Vpu-infected CD4 T cells (strain NLAD8) were subjected to antibody binding with indicated antibodies and surface levels were determined by flow cytometry (left panel). The staining obtained with mGO53 was similar to the background signal observed on unstained cells. Infected cells were cultured with the indicated antibodies and NHS for 24h and the C3 surface levels on infected (Gag+) cells were determined by flow cytometry (right panel). One representative experiment is shown."
NCBI gene IDs:
"Vpu: 155945"
Figure legend: "(A) Primary CD4 T cells were nucleofected with a control (CRISPR Control) or anti-CD59 (CRISPR CD59) guide RNA/Cas9 complex. CD59 surface levels were analyzed by flow cytometry 7 days after nucleofection. Isotype control (grey) and CD59 stainings (blue). One representative experiment is shown on the left. Numbers indicate the % of CD59+ cells (middle) or the MFI of staining in CD59- cells (Right). Each dot represents a single donor of CD4 T cells. n=6 donors of CD4 T cells."
NCBI gene IDs:
"Cas9: 57852564
CD59: 966"
Figure legend: "(B) NLAD8∆Vpu-infected CD59 KO primary CD4 T cells were cultured with indicated antibodies and NHS or HIHS for 24h. Numbers indicate the percentage of infected (Gag+) cells. One representative experiment is shown."
NCBI gene IDs:
"CD59: 966
Vpu: 155945"
Figure legend: "(C) CD59-KO (CRISPR CD59) or control (CRISPR Control) CD4 T cells infected with HIV WT (left) or HIVΔVpu (right) (strain NLAD8) were cultured with the indicated antibody, and NHS or HIHS for 24h. The % of CDC was calculated as the % of disappearance of Gag+ cells after culture with NHS compared to HIHS. Each dot represents a single donor of CD4 T cells. n= at least 6 donors of CD4 T cells."
NCBI gene IDs:
"CD59: 966
Vpu: 155945"
Figure legend: "(C) Frequency of live HIV-1-infected cells (NLAD8 WT or ΔVpu strain) 6 days after incubation with the indicated antibodies, and with NHS or HIHS. Each donor of CD4 T cells is represented by a different symbol. n=8 donors of CD4 T cells."
NCBI gene IDs:
"Vpu: 155945"
Figure legend: "(D) Elimination of HIV-1-infected cells (strain NLAD8 WT or ΔVpu) over culture with the indicated antibody (E430G), and NHS or HIHS. The frequency of % infected (Gag+) cells is determinined by flow cytometry. The mean relative percentage of infected cells compared to the HIHS condition is depicted. n=6-8 donors of CD4 T cells."
NCBI gene IDs:
"Vpu: 155945"
Figure legend: "(C) CD4 T cells not infected (NI) or infected with WT, ∆Nef, ∆Vpu or ∆Nef∆Vpu HIV-1 (strain CH058) were incubated with the serum of HIV-1-infected ART-treated individuals (sKB104 or sKB105, dilution 1:1000). The levels of IgG binding on infected (Gag+) cells were analyzed by flow cytometry. One representative experiment is shown on the left. On the right, results are expressed as MFI of staining. Each dot represents a single donor of CD4 T cells. n=6 donors of CD4 T cells. Error bars indicate SEM. *P&lt;0.05, Wilcoxon test. Only significant comparisons are depicted."
NCBI gene IDs:
"Nef: 156110
Vpu: 155945"
Figure legend: "(D) Primary CD4 T cells either not infected (NI) or infected with WT, ∆Nef, ∆Vpu or ∆Nef∆Vpu HIV-1 (strain CH058) were incubated with 50% NHS from a healthy donor and serum of HIV-1-infected ART-treated individuals (sKB104 or sKB105, dilution 1:100). The levels of C3 deposition on infected (Gag+) cells were analyzed by flow cytometry. One representative experiment is shown (left panel). Results are expressed as MFI of staining (right panel). Each dot represents a single donor of CD4 T cells. n=6 donors of CD4 T cells."
NCBI gene IDs:
"Nef: 156110
Vpu: 155945"
Figure legend: "(E) Correlation between patients' sera (sK104 and sKB105) antibody binding and C3 deposition on CD4 T cells either mock-infected or infected with WT, ∆Nef, ∆Vpu or ∆Nef∆Vpu HIV-1 (strain CH058). Each dot is the mean of 6 donors of CD4 T cells. Correlation was analyzed by Spearman correlation. Correlation coefficient (r) and p-value are indicated."
NCBI gene IDs:
"Nef: 156110
Vpu: 155945"
Figure legend: "Primary brown adipocytes were transfected with MPC1 siRNA (MPC1 KD) or Scramble RNA (Scramble). (C) Representative Western Blot analysis of MPC1, MPC2 and actin. (D) Quantification of MPC1 and MPC2 expression normalized to actin from Western Blots in (C) from n=3 individual experiments."
NCBI gene IDs:
"MPC1: 55951"
Figure legend: "Primary brown adipocytes were transfected with MPC1 siRNA (MPC1 KD) or Scramble RNA (Scramble). (E) mRNA levels of MPC1 and MPC2 from n=3 individual experiments."
NCBI gene IDs:
"MPC1: 55951
MPC2: 70456"
Figure legend: "Primary brown adipocytes were transfected with MPC1 siRNA (MPC1 KD) or Scramble RNA (Scramble). (F) Representative OCR traces of differentiated primary brown adipocytes averaging 6 technical replicates. OCR were measured in respirometry media supplemented with 5 mM glucose and 3 mM glutamine. Norepinephrine (NE; 1 µM), oligomycin A (Oligo; 4 µM), etomoxir (Eto; 40 µM) and antimycin A (AA; 4 µM) were injected where indicated. (G) Quantification of basal, NE-stimulated and Eto-sensitive OCR as shown in (F) from n=7 individual experiments. Data were normalized to Scramble RNA for each individual experiment. (H) Quantification of basal OCR in response to 100 nM UK5099 treatment in Scramble RNA of MPC1 siRNA transfected cells from n=5 individual experiments. Data were normalized to vehicle for each experiment."
NCBI gene IDs:
"MPC1: 55951"
Figure legend: "(C-E) Effect OGC1 downregulation on UK5099-induced energy expenditure. Primary brown adipocytes were transduced with Scramble RNA (Scramble) or shOGC1 (OGC1 KD) adenovirus. Cells were pre-treated for 2 h with vehicle (DMSO) or 100 nM UK5099 before OCR measurements. OCR were measured in respirometry media supplemented with 5 mM glucose and 3 mM glutamine in the presence of vehicle or UK5099. Norepinephrine (NE; 1 µM,) oligomycin A (Oligo; 4 µM), etomoxir (Eto; 40 µM) and antimycin A (AA; 4 µM) were injected where indicated. (C) Representative OCR traces averaging 4 technical replicates. (D) Quantification of non-stimulated OCR as measured in (C) from n=6 individual experiments. (E) Quantification of Eto-sensitive OCR as measured in (C) from n=6 individual experiments. Data were normalized to vehicle for each experiment."
NCBI gene IDs:
"OGC1: 67863"
Figure legend: "(F-H) Effect of Aralar1 downregulation on UK5099-induced energy expenditure. Primary brown adipocytes were transfected with Scramble RNA (Scramble) or siRNA for Aralar1 (Aralar1 KD). Cells were pre-treated for 2 h with vehicle (DMSO) or 100 nM UK5099 before OCR measurements. OCR were measured in respirometry media supplemented with 5 mM glucose and 3 mM glutamine in the presence of vehicle or UK5099. Norepinephrine (NE; 1 µM), oligomycin a (Oligo; 4 µM), etomoxir (Eto; 40 µM) and antimycin a (AA; 4 µM) were injected where indicated. (F) Representative OCR traces averaging 4 technical replicates. (G) Quantification of non-stimulated OCR as measured (G) from n=4 individual experiments. Data were normalized to vehicle for each experiment. (H) Quantification of Eto-sensitive OCR as measured in (F) from n=4 individual experiments."
NCBI gene IDs:
"Aralar1: 78830"
Figure legend: "(I) Brown adipocytes transfected with Scramble RNA or Aralar1 siRNA were treated for 24 hours with vehicle (DMSO) or 10 µM UK5099. Data shows quantification of the ratio of aspartate to glutamate abundance as measured by GC-MS from n=3 individual experiments."
NCBI gene IDs:
"Aralar1: 78830"
Figure legend: "B Plasmids encoding truncated mutants of the Smad6 MH2 domain were co-transfected into HEK293 cells with HA-tagged Pellino-1N plasmid. Cell lysates were immunoprecipitated with anti-HA antibody and immunoblotted with anti-Myc antibody. Data are representative of at least three independent experiments. IP, immunoprecipitation; IB, immunoblot; TCL, total cell lysates."
NCBI gene IDs:
"Smad6: 4091"
Figure legend: "C, D The SBE-Luc reporter plasmid was co-transfected with an empty vector, Myc-Smad6 (400-441), or full-length Smad6-expressing plasmids into CMT-93 cells, respectively. After 24 h, cells were treated with TGF-β1 for 2 h and luciferase activities were measured and normalized. Data were statistically analyzed by a t-test and show the mean ± SD of three independent experiments. **P &lt; 0.05, ***P &lt; 0.001."
NCBI gene IDs:
"luciferase: 
Smad6: 17130
Smad6: 4091"
Figure legend: "C, D The 5xNF-κB-Luc reporter plasmid was co-transfected with an empty vector, Myc-Smad6 (400-441), or full-length Smad6-expressing plasmids into CMT-93 cells, respectively. After 24 h, cells were treated with LPS for 2 h and luciferase activities were measured and normalized. Data were statistically analyzed by a t-test and show the mean ± SD of three independent experiments. **P &lt; 0.05, ***P &lt; 0.001."
NCBI gene IDs:
"luciferase: 
Smad6: 17130
Smad6: 4091"
Figure legend: "E CMT-93 cell lines stably expressing Smad6 amino acids 400-441 were treated with LPS for the indicated time and expression of IκBα, IKKα, and phospho-IKKα/β was monitored by immunoblotting. As a positive control, CMT-93 cells were pre-treated with TGF-β1 for 2 h. CMT-93 cells stably expressing the empty vector pMSCV-puro were used as a negative control. β-actin was used as a loading control. All data are representative of three independent experiments."
NCBI gene IDs:
"Smad6: 4091"