EMBO/SourceData huggingface dataset:
Figure legend: "Western blot for cleaved parp and vinculin (vinc) in PHD1-silenced HCT116 treated for 20 h with 300 μM 5-FU alone or in combination with 3 μg/ml α-amanitin."
NCBI gene IDs:
"PHD1: 112398"
Figure legend: "PHD1 mRNA levels in p53R248/− HCT116 silenced for PHD1. *P = 0.0005 toward scrambled control with a two-tailed unpaired t-test and n = 3/group."
NCBI gene IDs:
"PHD1: 112398
p53: 7157"
Figure legend: "Western blot for p53 pS15, p53, and vinculin in PHD1-silenced p53R248/− HCT116 upon 8-h treatment with 300 μM 5-FU."
NCBI gene IDs:
"PHD1: 112398
p53: 7157"
Figure legend: "Western blot for cleaved casp3 and vinculin in p53R248/− HCT116 silenced for PHD1 and treated with 300 μM 5-FU for 24 h."
NCBI gene IDs:
"PHD1: 112398
p53: 7157"
Figure legend: "Western blot for pH2AX and vinculin (vinc) in p53wt/wt and p53−/− HCT116 cells after 24-h treatment with 300 μM 5-FU."
NCBI gene IDs:
"p53: 7157"
Figure legend: "Detection of pH2AX and vinc in p53S15A→p53−/− HCT116 cells silenced for PHD1 and treated with 300 μM 5-FU for 24 h."
NCBI gene IDs:
"PHD1: 112398
p53: 7157"
Figure legend: "Detection of pH2AX and vinc in HCT116 silenced with a second silencing construct of PHD1 and treated for 24 h with 300 μM 5-FU."
NCBI gene IDs:
"PHD1: 112398"
Figure legend: "Western blot for pH2AX and vinc in p53R248/− HCT116 cells silenced for PHD1 upon treatment with 300 μM 5-FU for 24 h."
NCBI gene IDs:
"PHD1: 112398
p53: 7157"
Figure legend: "Detection by Western blot of p53 and XPB from whole cell extracts (WCE) or after immunoprecipitation of p53 from cells silenced for Scr control or siPHD1 and treated for 4 or 8 h with 300 μM 5-FU."
NCBI gene IDs:
"PHD1: 112398"
Figure legend: "Western blot for pH2AX and vinc in HCT116 silenced for PHD1 alone or in combination with silencing for XPB upon treatment with 300 μM 5-FU for 20 h."
NCBI gene IDs:
"PHD1: 112398
XPB: 2071"
Figure legend: "B Western blots and ligand-overlay (O/L) of wheat-germ agglutinin-enriched muscle and fibroblasts lysates from PII.2, PII.5, the healthy sibling (HII.3) and healthy controls (Ctr, Ctr1, and Ctr2) (Muscle: 250μg protein/lane, Fibroblasts: 800μg/lane). In muscle, expression of αDG-IIH6 is reduced but the αDG-Core shows similar expression as controls, with a slight reduction in molecular weight; laminin overlay assay detected some binding activity but was diminished compared to controls, whereas agrin-binding activity showed no difference with controls. In fibroblasts, both αDG-IIH6 and αDG-Core expression and laminin-binding activity were normal, suggesting that unlike other muscular dystrophies, α-dystroglycan glycosylation defect is muscle-specific in POGLUT1 patients."
NCBI gene IDs:
"POGLUT1: 56983"
Figure legend: "A Protein O-glucosyltransferase activity of wild-type (WT) or D233E mutant POGLUT1 protein toward human factor IX EGF repeat (hFIX-EGF). Wild-type shows higher O-glucosyltransferase activity than D233E, and this activity is dependent on the concentration of the acceptor substrate hFIX-EGF repeat (left) and on the concentration of donor substrate UDP-glucose (UDP-Glc) (right). Values indicate mean ± SEM from three independent assays."
NCBI gene IDs:
"POGLUT1: 56983"
Figure legend: "B O-glucosyltransferase activity towards five different single EGF repeats from mouse Notch1. Wild-type POGLUT1 shows higher activity toward all EGF repeats tested than POGLUT1D233E. The Y-axis shows the normalized activity relative to wild-type POGLUT1. EGF repeats were at 10 μM. Values indicate mean ± SEM from three independent assays."
NCBI gene IDs:
"POGLUT1: 56983"
Figure legend: "C Elution profiles of the POGLUT1 reaction products on reverse phase HPLC. hFIX-EGF repeat was incubated with wild-type or D233E mutant POGLUT1 and donor substrate, UDP-Glc or UDP-xylose (UDP-Xyl), at 37ºC overnight. Values on top of the peaks indicate the measured masses. Addition of Glc (162 Da) or xylose (132 Da) to hFIX-EGF (5696.2 Da) by wild-type POGLUT1 caused a shift to an earlier retention time (left). Similarly, the products exhibited a similar shift after incubation with POGLUT1D233E (right). These results indicate that POGLUT1D233E can add a single glucose or xylose to hFIX-EGF repeats and thus has residual enzymatic activity."
NCBI gene IDs:
"POGLUT1: 56983"
Figure legend: "B-C qRT-PCR on RNA extracted from skeletal-muscle shows that expression of HES1 (B) and PAX7 (C) was significantly lower in D233E patients compared to healthy controls. Mean ± SEM, Student´s t-test (B) and Mann-Whitney U test (C)."
NCBI gene IDs:
"HES1: 3280
PAX7: 5081"
Figure legend: "A-A\" Staining of Drosophila indirect flight muscles at 25% pupal development with 22C10 antibody (myotube marker) and anti-Twist antibody (myoblast marker) indicates that in wild-type animals, indirect flight muscles are formed from 6 myotubes, and a large number of Twist+myoblasts are present.B-B\" rumi79/79 mutants raised at 18C show no obvious defects in myoblast number or myotube morphology.C-D\" rumi79/79 mutants exhibit decreased number of myoblasts at 25°-30°C and either an incomplete set of short myotubes when raised at 25C or aberrant morphology of myotubes when raised at 30C.E-E\" rumi79/79 animals overexpressing FLAG-tagged POGLUT1WT in the muscle compartment using Mef2-GAL4 and raised at 25C during the pupal stage show a rescue of the rumi79/79 muscle phenotype.F-G\" Overexpression of FLAG-tagged POGLUT1D233E only partially rescues the rumi79/79 muscle phenotype, with some variation in the myotube length and the number of restored Twist+cells (compare G and F). Note that the number of Twist+cells restored by POGLUT1D233E is much smaller than that restored by POGLUT1WT. Scale bar in A' is 50 µm and applies to A-G\".H Quantification of myotube lengths in rumi79/79 mutants with or without POGLUT1 expression indicates a weak rescue of the phenotypes by POGLUT1D233E compared to POGLUT1WT. Mean ± standard deviation is shown. The differences in myotube lengths are statistically significant (n = 22-54). Mean ± standard deviation is shown; One-way Anova with Bonferroni´s multiple comparisons test."
NCBI gene IDs:
"GAL4: 855828
Mef2: 36032
POGLUT1: 56983
rumi: 326122"
Figure legend: "C-D C2C12 myogenic cells showed a reduced level of glycosylated α-dystroglycan when the Notch signaling inhibitor DAPT or LY3039478 was added to the differentiation medium. This indicates that reducing Notch signaling can affect α-dystroglycan glycosylation in wild-type C2C12 myoblasts cells, which do not harbor any known mutations in α-dystroglycan glycosyltransferases or Poglut1."
NCBI gene IDs:
"Poglut1: 56983"
Figure legend: "E-F qRT-PCR experiments show that upon DAPT or LY3039478 treatment, a remarkable decrease in Hes1 mRNA is induced in C2C12 cells during differentiation."
NCBI gene IDs:
"Hes1: 15205"
Figure legend: "A panel of selected breast cancer and near normal cell lines was reverse-transfected with 5 nM pooled CEP55 siRNAs and cell viability determined after 6 days. Cell viability relative to its own respective control transfected with scramble siRNA was calculated. Graph represents the mean± SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Immunoblot analysis of CEP55 expression in CEP55 knockdown MDA-MB-231 cells. Two isogenic lines (sh#2 and sh#8) were obtained using two different shRNA sequences as described in method section. COX-IV served as loading control."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Effect of CEP55 knockdown on cell proliferation in MDA-MB-231 cells assessed using the IncuCyte ZOOM® live cell imager (phase-only processing module). The percentage of cell confluence was determined using an IncuCyte mask analyzer. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Representative images of colony forming capacity at 14 days determined using crystal violet staining in control and CEP55 knockdown MDA-MB-231 cells."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Six-week-old female NOD/SCID cohorts of mice were injected in the 4th inguinal mammary fat-pad with the control and CEP55 knockdown cells. Growth rate (area, mm2) of the tumors was measured using digital calliper. Differences in growth were determined using Student's t test. Graph represents the mean tumor area±SEM, n=6 mice/group."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Immunoblots analysis of CEP55 protein expression in MCF10A cells stably transfected with a Flag-CEP55 expression construct and two isogenic lines were obtained (CEP55-#16 and CEP55-#17) with its respective empty vector (EV) transfected parental cells. COX-IV served as a loading control."
NCBI gene IDs:
"Flag: 
CEP55: 55165"
Figure legend: "Effect of CEP55 overexpression on cell proliferation in MCF10A assessed using the IncuCyte ZOOM® live cell imager as described in panel E. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Representative images of colony forming capacity at 14 days determined using crystal violet staining in empty vector (EV) and CEP55-overexpressing (CEP55#17 and CEP55#18) lines."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Representative images of single plane phase-contrast and Z-stacked immunofluorescence of empty vector (EV) and CEP55-overexpressing MCF10A cells grown on Matrigel for 14 days. Red: Cytokeratin-19; Green: F-actin stained by Phallodin and Blue: DAPI. Z-stack images were acquired through Zeiss LSM 780 confocal microscope-ZMBH."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(A) Polyploidy analysis (&gt;4N DNA contents) determined using FACS in control and CEP55 knockdown MDA-MB-231 cells (left panel). Representative corresponding cytogram showing different phases of cell cycle and the polyploidy subpopulation analyzed using ModFit LT 4.0 software (right panel). Yellow peaks represent subpopulation of polyploidy/aneuploidy. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(B) Analysis of chromosome number in control and CEP55 knockdown MDA-MB-231 cells (sh#8) by Giemsa staining of metaphase spreads (left panel). Representative images showing chromosome spreads assessed by Zeiss AxioScope2 microscopy (right panel). n= number of metaphase spreads counted (mean ±95% confidence interval of two independent experiments)."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(C) Representative SNP array plots showing chromosomal alterations following CEP55-knockdown in MDA-MB-231 cells. BAF is B allele frequency, value between 0 and 1 and represents the proportion contributed by one SNP allele (B) to the total copy number: BAF is an estimate of NB/(NA+ NB), where NA and NB are the number of A and B alleles, respectively."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(D) Percentage of genome altered evaluated using amplified copies (6-8 copies across genome) in both parental and CEP55 knockdown cells determined using data from SNP arrays. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(E) SNP array plots showing chromosomal alterations following CEP55 overexpression in MCF10A cells. Red box indicates an extra chromosome 20 in CEP55-overexpressing cells."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(G) Kaplan-Meier survival analysis of METABRIC data showing the relationship between CEP55 expression and survival. Cases were subdivided into tertiles. Log-rank (Mantel-Cox) test, P&lt; 0.0001."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(A) Representative cytogram of control and CEP55 knockdown MDA-MB-231 cells showing cell cycle profiles following treatment with and without the B12536 (5 nM)."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Percentage of polyploidy (&gt;4N) following B12536 (5 nM) in control and CEP55 knockdown MDA-MB-231 cells. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "Percentage of polyploidy (&gt;4N) following nocodazole (0.5 µM) in control and CEP55 knockdown MDA-MB-231 cells. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(F) Percentage of sub-G1 fraction following B12536 (10 nM) or nocodazole (0.5 µM) in control and CEP55 knockdown Hs578T cells. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(G) Representative cytogram of empty vector (EV) and CEP55-overexpressing MCF10A cells showing cell cycle profile following treatment with and without the B12536 (10 nM)."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(H) Percentage of polyploidy following treatment with and without B12536 (10 nM) in empty vector (EV) and CEP55-overepressing MCF10A cells. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(A) Box and whiskers plot showing average time spent in mitosis and (B) mitotic outcomes in control and CEP55 knockdown MDA-MB-231 cells following treatment with the BI2536 (5 nM). Time taken to complete mitosis was defined as the time from nuclear envelope breakdown until two daughter cells were observed whereas mitotic slippage or death was defined as cells that prematurely exited mitosis with a flattened and a multinucleated phenotype or died during mitosis, characterized by membrane blebbing. Graph represents the mean±SEM of two independent experiments. For each experiments n=50 mitotic cells were counted per condition using Olympus Xcellence IX81 time-lapse microscopy."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(G) Representative mCerulean lifetimes maps of Hs578T control and CEP55 knockdown cells upon nocodazole (0.5 µM) treatment at indicated time points. Cells were synchronized using double thymidine for 16 hours prior to nocodazole treatment."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(H) Quantification of mCerulean lifetimes of control and Hs578T CEP55 knockdow ncells and in response to treatment with nocodazole at indicated time points as shown in G. Blue: 1 ns; red: 3 ns. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(I) Control and CEP55 knockdown MDA-MB-231 cells were synchronized as above, released into culture medium for six hours and then blocked with nocodazole (0.5 µM) for an additional four hours prior to treatment with the Cdk1 inhibitor RO-3306 for an additional 16 hours. Sub-G1 cells were then identified by propidium iodide staining and quantified by FACS. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(J) Control and CEP55 knockdown MDA-MB-231 cells were transfected with MAD2 siRNA (5 nM) for 48 hours followed by nocodazole (0.5 µM) treatment for an additional 12 hours. Cells were collected, fixed and sub-G1 analysis was performed as described in methods. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165
MAD2: 10459///4085"
Figure legend: "(C) Relative CEP55 promoter luciferase activity in MDA-MB-231 cells either treated with AZD6244 (1 µM) for 6 hours or ERK1/2 knockdown for 24 hours. PGL basic reporter plasmid was used to normalize basal CEP55 promoter activity. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165
ERK1: 5595"
Figure legend: "(E) Relative CEP55 promoter luciferase activity upon MYC siRNA was determined using DualGlo assay in MDA-MB-231 cells similar to experiment in panel C. Graph represents the mean±SEM of two independent experiments."
NCBI gene IDs:
"CEP55: 55165
MYC: 4609"
Figure legend: "(F) Immunoblots analysis showing MYC and CEP55 expression in MDA-MB-231 cells upon transfection with siRNA against MYC, and ETS1 (10 nM), with and without EGF (100 ng/mL) stimulation as indicated time points. COX-IV served as a loading control."
NCBI gene IDs:
"ETS1: 2113
MYC: 4609"
Figure legend: "(G) Immunoblots analysis showing CEP55 and MYC levels following 4-hydroxytamoxifen (4OHT) (0.5 µM) induction in MCF10A MYCER cells cultured in 0.1% fetal bovine serum contained media at indicated time points. COX-IV served as a loading control."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(I) Analysis of Pearson correlation coefficients (PCC) of CEP55 vs. MYC transcriptional activity in breast cancer TCGA tumor data with R=0.4157, p&lt;0.0001."
NCBI gene IDs:
"CEP55: 55165
MYC: 4609"
Figure legend: "(J) Both control and CEP55 knockdown MDA-MB-231 cells were exposed with different concentrations of PLK1 (BI2536) alone (i) or in combination with AZD6244 (1 µM) (ii-iv), and cell viability was determined after 6 days. The dose-response curve was generated by calculating cell viability relative to untreated control and plotted against drug concentration. Graph represents the mean±SEM of three independent experiments."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(L) Immunoblots analysis of both control and CEP55 knockdown MDA-MB-231 cells treated with single and combination treatments after 96h and cleaved PARP, Caspase-3 along with MYC, ERK1/2 and CEP55 were determined. COX-IV as a loading control."
NCBI gene IDs:
"CEP55: 55165"
Figure legend: "(A) Left, Six week-old female BALB/c cohorts of mice were injected in the 4th inguinal mammary fat pad with the Cep55-ovexpressing mammary carcinoma cell line 4T1.2. Tumor size (area, mm2) was measured using a digital calliper and mean tumor size of each cohort is presented. Mice were treated with vehicle, AZD6244 (12.5mg/kg BID), BI6727 (12.5mg/kg thrice weekly), or combined AZD6244 and BI2536 treatment. Right, representative excised tumor images are shown. Graph represents the mean tumor area±SEM from six mice/group."
NCBI gene IDs:
"Cep55: 74107"
Figure legend: "E: Colocalization  assays in Kms27  and RPMI8226 MM  cell lines by combining  In Situ Hybridization (FISH) staining  of NEAT1   (Stellaris) with immunofluorescence  of Che-1. Scale bar 10 μm."
NCBI gene IDs:
"NEAT1: 283131"
Figure legend: "F: (Left) RT-qPCR analysis of NEAT1  expression levels in Kms27 MM  cells transiently transfected with LNA GapmeR NEAT1A  or GapmeR Control oligonucleotides. Values were normalized to Actin  expression. Error bars represent the standard error of three different biological experiments performed in technical duplicate (Two Tailed T test ****P&lt;0.001). (Right) Nuclear  extracts from KMS27 MM  cells were immunoprecipitated  with the indicated abs and analyzed by WB with the indicated abs."
NCBI gene IDs:
"Actin: 60
NEAT1: 283131
NEAT1A: 283131"
Figure legend: "A: Quantitative RT-PCR (RT-qPCR) of Kms27 MM  cells transiently transfected with two different LNA GapmeR NEAT1   or GapmeR Control oligonucleotides. Values were normalized to Actin  expression. Error bars represent the standard error of three different experiments performed in duplicate (Two Tailed T test ****P&lt;0.001)."
NCBI gene IDs:
"Actin: 60
NEAT1: 283131"
Figure legend: "C: Representative confocal  images of Kms27 MM  cells transiently transfected with LNA GapmeR NEAT1A, GapmeR NEAT1B  or GapmeR Control oligonucleotides and immunostained  with anti-Che-1 antibody. Scale bar 10 μm."
NCBI gene IDs:
"NEAT1A: 283131
NEAT1B: 283131"
Figure legend: "D: Isolated cell compartments from Kms27 MM  cells transfected with LNA GapmeR NEAT1A  or GapmeR Control oligonucleotides, were subjected to WB  with the indicated abs."
NCBI gene IDs:
"NEAT1A: 283131"
Figure legend: "E: Che-1 ChIP-seq  signal intensity of LNA GapmeR NEAT1A  (2 replicates on right) and GapmeR Control (2 replicates on left) and at  co-localizing Che-1/NEAT1  sites (N=14654 sites). Signal intensity from 1 (weak) to 12 (strongest)."
NCBI gene IDs:
"NEAT1: 283131
NEAT1A: 283131"
Figure legend: "F: FISH  images of Kms27 MM  cells depleted or not for  Che-1  expression (siChe-1A) labeled with Hoechst  (cell nuclei) and Quasar 670 (NEAT1  RNA). Scale bar 10 μm."
NCBI gene IDs:
"Che-1: 26574
Che-1A: 26574
NEAT1: 283131"
Figure legend: "G: NEAT1 ChIRP-seq signal intensity of Kms27 MM  cells transiently transfected with siChe-1A or siControl at co-localizing Che-1/NEAT1  sites (N=14654 sites). Signal intensity from 1 (weak) to 14 (strongest)."
NCBI gene IDs:
"Che-1A: 26574
NEAT1: 283131"
Figure legend: "D: Chromatin  extracts from Kms27 MM  cells transfected with LNA GapmeR NEAT1A  or GapmeR Control oligonucleotides were immunoprecipitated  with  S9.6 antibody and analyzed by WB  with the indicated antibodies. Input corresponds to 5% of the total extract used for immunoprecipitation."
NCBI gene IDs:
"NEAT1A: 283131"
Figure legend: "E: (Left) Representative confocal  images of Kms27 MM  cells treated or not with RNase H, transiently transfected with siChe-1A or siControl and immunostained  with  S9.6 antibody and. Scale bar 10 μm. (Right) Box plot showing S9.6  signal nuclear  intensity per cell (Arbitrary Unit-AU). siChe-1A  cells are described in green color, while siControl in grey color. 100 cells were counted in each replicate. Each dot represents a cell. Median scores per sample are written at the top of the median red line. P value: &lt;1.02-18. ANOVA test was performed to evaluate statistical significance. The red center line denotes the median value (50th percentile) while the black box contains the 25th to 75th percentiles of data. The whiskers mark the 5th and 95th percentiles."
NCBI gene IDs:
"Che-1A: 26574"
Figure legend: "G: Enrichment density of DRIP-seq  signals at NEAT1/Che1 co-localizing sites in Kms27 MM  cells identified in siRNA control (siControl) (grey) and siRNA Che-1 (siChe-1A) (dark green) samples. Enrichment density was calculated on the relative size of each peak (start to end of each fragment) extended 2000 bp upstream and downstream. Right frame represents the signal obtained from 14654 randomly selected sites from the same experiments."
NCBI gene IDs:
"Che-1: 26574
Che-1A: 26574
NEAT1: 283131"
Figure legend: "D: (Top) Representative confocal   images of Kms27 MM   cells transfected and treated as in C and immunostained   with S9.6  antibody. Scale bar 10 μm. (Bottom) Box plot showing S9.6  signal nuclear   intensity per cell (Arbitrary Unit-AU). siChe-1A cells are described in dark grey, siChe1A plus CX5461   in green color, while siControl in grey color. Median scores per sample are written at the top of the median red line. P value: &lt;1.30-54. ANOVA test was performed to evaluate statistical significance. The red center line denotes the median value (50th percentile) while the black box contains the 25th to 75th percentiles of data. The whiskers mark the 5th and 95th percentiles."
NCBI gene IDs:
"Che-1A: 26574
Che1A: 26574"
Figure legend: "E: (Top) Dot blot  analysis of decreasing amounts of genomic DNA   extracts from Kms27 MM  cells depleted or not for Che-1 expression (siChe-1A) and treated with CX5461, were probed using abs directed against RNA:DNA hybrids. (Bottom) Quantification of S9.6 levels by  densitometric analysis. Values were normalized to a Methylene blue  loading control. Error bars represent the standard error of three different biological experiments (Two Tailed T test ****P&lt;0.001)."
NCBI gene IDs:
"Che-1: 26574
Che-1A: 26574"
Figure legend: "A: Differential analysis of siControl vs. siChe1A transcriptome in Kms27 MM  cells. Volcano plot shows 135 significantly upregulated (dark green) and 392 downregulated genes (dark grey). x-axis reports the base-2 logarithm of fold change which is approximated to the B value coming from the statistical Wald test. y-axis reports the base-10 logarithm of Q value of significant genes."
NCBI gene IDs:
"Che1A: 26574"
Figure legend: "D: WB  analysis of total extracts of Kms27 MM  cells depleted or not for Che-1 (siChe-1A) and probed for the indicated abs."
NCBI gene IDs:
"Che-1: 26574
Che-1A: 26574"