EMBO/SourceData huggingface dataset:
Figure legend: "Alizarin Red (bone) (J) staining of scramble and Fam134bmo medaka fish. Graph shows quantification of Ethmoid plate (EP), Palatoquadrate (PQ), Ceratohyal (CH), Paired Prootics (PO), Ceretobranchials 1 to 5 (CB1 to CB5) bone mineralization (J) in Fam134bmo and scramble fish. Values were expressed as % relative to the scramble (100% red dotted line). Mean +/- standard error of the mean (sem) of n=9 fish/genotype. Student unpaired T-Test *p&lt;0.05; **p&lt;0.005; ***p&lt;0.0005. NS not significant."
NCBI gene IDs:
"Fam134b: 101158885"
Figure legend: " (A) Bcl-xfl/fl;RosaCreERT2+/Ki (n=12) or, as controls, Bcl-xfl/fl (n=6) and RosaCreERT2+/Ki (n=16) mice (age 9-12 weeks, males and females) were treated with tamoxifen (200 mg/kg/body weight administered in 3 daily doses by oral gavage) to induce CreERT2-mediated deletion of the floxed Bcl-x alleles. Mice were monitored for up to 200 days post-treatment with tamoxifen. Data are presented as % survival post-treatment with tamoxifen and statistical significance was assessed using the Mantel-Cox (Log-rank) test; ****p&lt;0.0001. "
NCBI gene IDs:
"Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (B) Bcl-xfl/fl;RosaCreERT2+/Ki (n=24) or, as controls, Bcl-xfl/fl (n=9) and RosaCreERT2+/Ki (n=6) mice (males and females, aged 8-14 weeks, numbers also indicated in the figure legend) were lethally γ-irradiated (2 x 5.5 Gy, 3 h apart) and reconstituted with bone marrow from UBC-GFP mice (referred to as GFP-Chimeras). After 8 weeks, reconstituted mice were treated with tamoxifen (200 mg/kg body weight administered in 3 daily doses oral gavage) and monitored for up to 200 days (termination of the experiment). Data are presented as % survival post-treatment with tamoxifen and statistical significance was assessed using the Mantel-Cox (Log-rank) test; ****p&lt;0.0001. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417
UBC: 22190"
Figure legend: " (C-F) Total counts of (C) platelets, (D) red blood cells (RBC), (E) hemoglobin (HGB) content and (F) hematocrit (HCT) of tamoxifen-treated Bcl-xfl/fl;RosaCreERT2+/Ki (n=8) or, as controls, Bcl-xfl/fl (n=6) and RosaCreERT2+/Ki (n=12) and Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimera (n=21) or, as controls, Bcl-xfl/fl;GFP-Chimera (n=6) and RosaCreERT2+/Ki;GFP-Chimera (n=6) mice were determined by ADVIA. Data are presented as mean ±SEM. Each data point represents an individual mouse. Statistical significance was assessed using the Student's t-test; ****p&lt;0.0001. (G) Spleen weights were measured in sick mice (at sacrifice) or, for the healthy control mice, at the termination of the experiment. Data are presented as mean ±SEM. Each data point represents an individual mouse and numbers are indicated. Statistical significance was assessed using the Student's t-test; ****p&lt;0.0001. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (H) Representative image of enlarged spleens from two Bcl-xfl/fl;RosaCreERT2Ki/+ mice and age-matched control Bcl-xfl/fl and RosaCreERT2+/Ki mice (34 days after treatment). "
NCBI gene IDs:
"Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: "Bcl-xfl/fl;RosaCreERT2+/Ki (n=8) or, as controls, Bcl-xfl/fl (n=6) and RosaCreERT2+/Ki (n=12) mice as well as Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=21), or as controls, Bcl-xfl/fl;GFP-Chimeras (n=6) and RosaCreERT2+/Ki;GFP-Chimeras (n=6) (age 8-14 weeks, males and females) were treated with tamoxifen (200 mg/kg/body weight administered in 3 daily doses by oral gavage) to induce CreERT2-mediated deletion of the floxed Bcl-x alleles. (A) Total white blood cell counts (WBC) were analyzed by ADVIA in sick mice or at the termination of the experiment (healthy control mice). Data are presented as mean ±SEM. Each data point represents an individual mouse and n numbers are indicated above. Statistical significance was assessed using the Student's t-test. No statistically significant differences were observed. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (B) The neutrophil/lymphocyte ratio (NLR) is presented as mean ±SEM for sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=21) or healthy control RosaCreERT2+/Ki;GFP-Chimeras (n=6) at the termination of the experiment. Data are presented as mean ±SEM. Each data point represents an individual mouse. Statistical significance was assessed using the Student's t-test; *p&lt;0.05. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (C) Histological analysis of H&amp;E-stained sections of the sternum of sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras or healthy wild-type and RosaCreERT2+/Ki;GFP-Chimera control mice at the indicated time points post-treatment with tamoxifen (dpt=days post-treatment). "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (D) Histological analysis of H&amp;E-stained sections of the livers of sick mice or age-matched healthy control wild-type mice or healthy RosaCreERT2+/Ki;GFP-Chimeras at the termination of the experiment (dpt=days post-treatment). "
NCBI gene IDs:
"Rosa: 
GFP: 
Cre: 2777477
ERT2: 26417"
Figure legend: " (E) ALT (left panel), AST (middle panel) and bilirubin levels (right panel) in the serum were determined in sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=18) or in healthy RosaCreERT2+/Ki;GFP-Chimeras (n=4) at the termination of the experiment. Data are presented as mean ±SEM. Each data point represents one individual mouse. Statistical significance was assessed using Student's t-test; **p&lt;0.01. nd=not detected. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " Bcl-xfl/fl;RosaCreERT2+/Ki (n=7) or as controls, Bcl-xfl/fl (n=8) and RosaCreERT2+/Ki (n=16) mice as well as Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=5), Bcl-xfl/fl;GFP-Chimeras (n=8) and RosaCreERT2+/Ki;GFP-Chimeras (n=6) (age 8-14 weeks, males and females) were treated with tamoxifen (200 mg/kg/body weight administered in 3 daily doses by oral gavage) to induce CreERT2-mediated deletion of the floxed Bcl-x alleles. (A) Kidney weights were measured in sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (at sacrifice) or, for the healthy control mice, at the termination of the experiment. Data are presented as mean ±SEM. Each data point represents one individual mouse. Statistical significance was assessed using Student's t-test; *p&lt;0.05, ***p&lt;0.001. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (B) Picture showing both kidneys from age-matched healthy control wild-type mice (left) and sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (right). "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (C) Histological analysis of H&amp;E-stained sections of the kidneys of age-matched healthy control mice or sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras as indicated, showing from left to right, shrunken scarred kidney, segmental chronic tubulo-interstitial disease, accumulation of cellular debris and amorphous material within the collecting ducts of the papilla. The higher magnification shows tubular epithelial degeneration, apoptosis and segmental secondary glomerular sclerosis. Pictures are representative of at least 5 mice for each genotype and treatment. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (D) Multiphoton analysis of fixed sections of the kidneys of age-matched healthy RosaCreERT2+/Ki;GFP-Chimeras (left) or sick Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (right). Pictures are representative of at least 3 mice for each genotype and treatment. green=second harmony, blue=short wavelength autofluorescence. "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (A) TUNEL staining of kidney sections from control RosaCreERT2+/Ki, Bcl-xfl/fl;RosaCreERT2+/Ki, RosaCreERT2+/Ki;GFP-Chimeras or Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras at the indicated time points post-tamoxifen treatment (200 mg/kg/body weight administered in 3 daily doses by oral gavage) or γ-irradiation (2 x 550 Rad), as indicated. Slides were counterstained with hematoxylin. Arrow heads indicate examples of TUNEL+ (apoptotic) cells. (B) TUNEL+ cells were quantified using a personalized script for the Image J software. Kidney sections were divided into ~40 microscopic images and the total numbers of blue and brown (TUNEL+) nuclei were determined. The percentages of TUNEL+ nuclei were calculated for each picture. The average value for each kidney section was then determined and plotted as a single data point. Error bars represent SEM from at least 3 independent samples for each genotype and treatment. Statistical significance was assessed using one-way ANOVA analysis with Tukey's multiple comparisons test (comparing control RosaCreERT2+/Ki (24 h post-tamoxifen n=2, 24 h post-γ-irradiation n=9) with Bcl-xfl/fl;RosaCreERT2+/Ki (24 h post-tamoxifen n=6, 24 h post-γ-irradiation n=5) and control RosaCreERT2+/Ki;GFP-Chimeras (24 h post-tamoxifen n=7, End point n=8) with Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (24 h post-tamoxifen n=8, End point n=6) (***p&lt;0.001). "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Cre: 2777477
ERT2: 26417"
Figure legend: " (A, B) Data are presented as % survival and statistical significance was assessed using the Mantel-Cox (Log-rank) test comparing tamoxifen-treated Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=25) with Bcl-xfl/fl;RosaCreERT2+/Ki;Bim+/-;GFP-Chimeras (n=25), Bcl-xfl/fl;RosaCreERT2+/Ki;Bim-/-;GFP-Chimeras (n=18), Bcl-xfl/fl;RosaCreERT2+/Ki;Puma+/-;GFP-Chimeras (n=21), Bcl-xfl/fl;RosaCreERT2+/Ki;Puma-/-;GFP-Chimeras (n=15) and Bcl-xfl/fl;RosaCreERT2+/Ki; Bim+/-;Puma+/-;GFP-Chimeras (n=22). *p&lt;0.05, ****p&lt;0.0001. The survival curves for the Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Bim: 12125
Cre: 2777477
Puma: 80885
ERT2: 26417"
Figure legend: " (D-F) (D) Total red blood cell (RBC) count, (E) hematocrit (HCT) and (F) hemoglobin (HGB) content were determined by ADVIA in the blood of sick mice of the indicated genotypes or at the termination of the experiment for healthy controls. Data are presented as mean ±SEM. Each data point represents one individual mouse. Statistical significance was assessed using Student's t-test comparing tamoxifen-treated RosaCreERT2+/Ki;GFP-Chimeras (n=6) with Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras (n=21), Bcl-xfl/fl;RosaCreERT2+/Ki;Bim+/-;GFP-Chimeras (n=20), Bcl-xfl/fl;RosaCreERT2+/Ki;Bim-/-;GFP-Chimeras (n=12), Bcl-xfl/fl;RosaCreERT2+/Ki;Puma+/-;GFP-Chimeras (n=15), Bcl-xfl/fl;RosaCreERT2+/Ki;Puma-/-;GFP-Chimeras (n=10) and Bcl-xfl/fl;RosaCreERT2+/Ki; Bim+/-;Puma+/-;GFP-Chimeras (n=17) ; ***p&lt;0.001; ****p&lt;0.0001. Data for the Bcl-xfl/fl;RosaCreERT2+/Ki;GFP-Chimeras and RosaCreERT2+/Ki;GFP-Chimeras "
NCBI gene IDs:
"GFP: 
Rosa: 
Bcl-x: 12048
Bim: 12125
Cre: 2777477
Puma: 80885
ERT2: 26417"
Figure legend: " (A) GFP-Chimeras were treated with the BCL-XL inhibitor A1331852 (n=8, 5 doses by oral gavage, 25 mg/kg body weight each dose). Control groups (n=8 each group) include untreated GFP-Chimeras and un-irradiated C57BL/6-Ly5.1 (wild-type) mice treated with the BCL-XL inhibitor A1331852 or left untreated. Mice were monitored for up to 200 days post-treatment (dpt). Data are presented as % survival. "
NCBI gene IDs:
"GFP: "
Figure legend: " Total red blood cell count (RBC), platelet count, hemoglobin (HGB) content and hematocrit (HCT) were determined by ADVIA as indicated in the blood of drug-treated GFP-Chimeras or control mice (n=8 each group) at the termination of the experiment Data are presented as mean ±SEM. Each data point represents one individual mouse. Statistical significance was assessed using one-way ANOVA analysis with Tukey's multiple comparisons test comparing untreated and drug-treated mice within each group (*p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&gt;0.0001). "
NCBI gene IDs:
"GFP: "
Figure legend: " (A) GFP-Chimeras were treated with the BCL-XL inhibitor A1331852 (n=8, 5 doses by oral gavage, 25 mg/kg body weight each dose). Control groups (n=8 each group) include untreated GFP-Chimera and un-irradiated C57BL/6-Ly5.1 (wild-type) mice treated with the BCL-XL inhibitor A1331852 or left untreated. Kidney (left panel) and spleen weights (right panel) were measured in drug-treated GFP-Chimeras or control mice at the termination of the experiment (n=8 each group). Data are presented as mean ±SEM. Each data point represents one individual mouse. Statistical significance was assessed using one-way ANOVA analysis with Tukey's multiple comparisons test. No statistically significant differences were observed. "
NCBI gene IDs:
"GFP: "
Figure legend: " (C) Histological analysis of H&amp;E-stained sections of the kidneys of drug-treated GFP-Chimeras or control mice at the termination of the experiment. Pictures are representative of at least 3 mice for each treatment group. "
NCBI gene IDs:
"GFP: "
Figure legend: "B Fluorescence images of cells expressing GPD promoter-driven GFP-Pho8 and the ER marker Sec63-mCherry and stained with CMAC to label the vacuole. The upper and lower panel show vacuole-associated and perinuclear GFP-Pho8 stretches and an intravacuolar GFP-Pho8 ring, respectively. Scale bars: 2 µm."
NCBI gene IDs:
"GPD: 853106"
Figure legend: "C Correlative light and electron microscopy of cells expressing GPD promoter-driven GFP-Pho8 and Sec63-mCherry. The lower panel shows the boxed area in the electron micrograph at higher magnification. The ER ring corresponds to a vacuolar whorl and the ER stretch to a perinuclear stack."
NCBI gene IDs:
"GPD: 853106"
Figure legend: "A Individual frames from time-lapse imaging of a cell expressing GAL promoter-driven GFP-Pho8 and stained with the vacuole membrane dye FM4-64. Numbers indicate the time in minutes after the start of the image sequence. Scale bar: 2 µm."
NCBI gene IDs:
"GAL: 852308"
Figure legend: "B Electron micrograph of a cell expressing GPD promoter-driven GFP-Pho8. Arrows indicate the edges of ER cisternae that are part of a bent stack in a vacuole membrane invagination. Black arrowheads point to vacuole membrane that encases the bent stack and the whorl."
NCBI gene IDs:
"GPD: 853106"
Figure legend: "Fluorescence images of ∆atg7 and ∆atg8 cells (A) expressing GAL promoter-driven GFP-Pho8 and the ER marker Sec63-mCherry and stained with CMAC to label the vacuole. Scale bar: 2 µm."
NCBI gene IDs:
"atg7: 856576
atg8: 852200
GAL: 852308"
Figure legend: "Fluorescence images of ∆snf7 and ∆vps4 cells (B) expressing GAL promoter-driven GFP-Pho8 and the ER marker Sec63-mCherry and stained with CMAC to label the vacuole. Scale bar: 2 µm."
NCBI gene IDs:
"GAL: 852308
snf7: 850712
vps4: 856303"
Figure legend: "C Relative activity of the ER-phagy reporter Yop1-Pho8∆60 in tunicamycin-treated cells. The dotted line indicates the activity in ∆atg7 cells. Mean + SEM, n ≥ 3."
NCBI gene IDs:
"atg7: 856576"
Figure legend: "D, E Relative activity of endogenous Pho8 in untreated cells (D) or after tunicamycin treatment (E). Background activity determined in ∆pho8 mutants was subtracted from the activity in all other strains. Mean + SEM, n = 3. WT, wild-type."
NCBI gene IDs:
"pho8: 852092"
Figure legend: "F, G Growth assays of untreated and tunicamycin-treated WT, ∆atg39/40, ∆snf7 and ∆snf7 ∆atg39/40 cells. Numbers indicate growth relative to WT cells based on the areas under the curves. Mean ± SEM, n = 3."
NCBI gene IDs:
"atg39: 851021
snf7: 850712"
Figure legend: "A Fraction of wild-type (WT), ∆vps23, ∆snf7 and ∆vps4 cells with GFP-Pho8 structures. Mean + SEM, n ≥ 3."
NCBI gene IDs:
"snf7: 850712
vps23: 850349
vps4: 856303"
Figure legend: "B Fraction of GFP-Pho8 structures classified as stacks, cytosolic whorls, uptake intermediates or vacuolar whorls. The number of GFP-Pho8 structures in WT, ∆vps23, ∆snf7 and ∆vps4 cells was 562, 345, 367 and 449, respectively. The table shows the results of Chi-Square tests for independence for all pairs of strains. ***, highly significant (P &lt; 0.00001); ns, not significant (P > 0.05)."
NCBI gene IDs:
"snf7: 850712
vps23: 850349
vps4: 856303"
Figure legend: "C Fraction of stacks classified as peripheral, perinuclear or vacuole-associated. The number of stacks in WT and ∆snf7 cells was 252 and 351, respectively. The observed distributions in the two strains were analyzed with a Chi-Square test for independence. ***, highly significant (P &lt; 0.00001)."
NCBI gene IDs:
"snf7: 850712"
Figure legend: "A Electron micrographs of wild-type and ∆snf7 cells treated with DTT for 3 h. Cy, cytosol; V, vacuole. Scale bars: 300 nm."
NCBI gene IDs:
"snf7: 850712"
Figure legend: "C, D Electron micrographs of serial thin sections of a ∆snf7 cell (C) or a wild-type cell (D) treated with DTT for 3 h. Note that the whorl appears separated from the cytosol in some sections (e.g. section 300 nm) but is still in contact with the cytosol (see sections 600 nm and 700 nm). In the wild-type cell (D), the vacuolar whorl is completely separated from the cytosol. Scale bar: 300 nm."
NCBI gene IDs:
"snf7: 850712"
Figure legend: "(C, D) qRT-PCR  analysis of Il2  and Ifng  mRNA in  CD4+ T cells from LN  and OB mice (C) or from  aged lean (AL, 17-month-old) and aged obese  (AO, 17-month-old) mice (D), then stimulated with TCR  (1/1 μg/ml) for 6 h. For AL  and AO mice, mice  were fed with a  normal diet  for 17 months, then divided into AL  (≤ 35 g) and AO  (≥ 45 g) mice  based on body weight (n = 3 or 4, biological replicates). Data information: All data are representative of at least 3 individual experiments. Statistics, two-tailed Student's t test; ns: not significant, *p &lt; 0.5, **p &lt; 0.01, ***p &lt; 0.001. Error bars represent SD."
NCBI gene IDs:
"Ifng: 15978
Il2: 16183"
Figure legend: "(S) BM12/SJL mice  (recipient, R) were adoptively transferred with CD4+ T cells from WT and Cpt1a-KO lean  or obese mice  (donors, D) to induce inflammation. The anti-nuclear  antibody (ANA) in serum  (top, scale bar: 50 μm) and the kidney IgG  deposition (bottom, scale bar: 50 μm) were visualized using immunofluorescence  (n = 4 or 5, biological replicates). Data are presented as Immunofluorescent  images (left) and quantification bar graphs (right). Data information: All data are representative of at least 3 individual experiments. Statistics, two-tailed Student's t test; ns: not significant, *p &lt; 0.5, **p &lt; 0.01, ***p &lt; 0.001. Error bars represent SD."
NCBI gene IDs:
"Cpt1a: 12894"
Figure legend: "(R) Endogenous ubiquitination  of Usp9x  in WT and Goliath-deficient CD4+ T cells that were left unstimulated  (-) or stimulated  (+) with α-CD3/28 (5/5 μg/ml)."
NCBI gene IDs:
"Goliath: 59044"
Figure legend: "(S) Co-IP  analysis of the interactions  between CaN  and Usp9x  or NFATc2  and CaN  in WT and Goliath-KO CD4+ T cells that were left unstimulated  (-) or stimulated  (+) with α-CD3/28 (5/5 μg/ml)."
NCBI gene IDs:
"Goliath: 59044"
Figure legend: "(A, B) Immunoblot  analysis of cytoplasmic  and nuclear  NF-AT levels in WT and Goliath-KO naïve  CD4+ T cells from lean  (LN) or obese  (OB) mice  that were stimulated by α-CD3/28 (5/5 μg/ml) or PMA/ionomycin  (P/I, 10/100 ng/ml)."
NCBI gene IDs:
"Goliath: 59044"
Figure legend: "(C-F) ECAR  measurements of glycolysis in WT and Goliath-deficient  naïve  CD4+ T cells treated with DMSO or fenofibrate  (FF, 5 μM) (C, D), or isolated from LN  or OB mice (E, F) that stimulated by α-CD3/28  (1/1 μg/ml) for 24 h (n = 3, biological replicates). The statistical results are presented as bar graphs (D, F). Data information: All data are representative of at least 3 individual experiments. Statistics, two-tailed Student's t test; ns: not significant, *p &lt; 0.5, **p &lt; 0.01, ***p &lt; 0.001. Error bars represent SD."
NCBI gene IDs:
"Goliath: 59044"
Figure legend: "(J-L) BM12/SJL mice  (recipient, R) were adoptively transferred with  CD4+ T cells from WT and Goliath-KO lean  or obese mice  (donors, D) to induce inflammation. The anti-dsDNA, anti-ssDNA  and anti-histone IgG  or IgM  in serum  were examined by ELISA (J). ANA in serum  (top, scale bar: 50 μm) and the kidney IgG  deposition (bottom, scale bar: 50 μm) were visualized using immunofluorescence  (n = 3, biological replicates). Data are presented as Immunofluorescent  images (K) and quantification bar graphs (L). Data information: All data are representative of at least 3 individual experiments. Statistics, two-tailed Student's t test; ns: not significant, *p &lt; 0.5, **p &lt; 0.01, ***p &lt; 0.001. Error bars represent SD."
NCBI gene IDs:
"Goliath: 59044"
Figure legend: "(L) Endogenous K48-linked ubiquitination  of HA-Goliath  in GOLIATH-overexpressed Jurkat T cell with or without Cpt1a  knockdown."
NCBI gene IDs:
"Cpt1a: 1374
GOLIATH: 55819"
Figure legend: "(M) Endogenous ubiquitination  of HA-Goliath  with indicated treatment in GOLIATH-knockdown  Jurkat T cell  reconstituted with HA-tagged GOLIATH  (WT) or Ring-truncated GOLIATH  (ΔR)."
NCBI gene IDs:
"HA: 
GOLIATH: 55819"
Figure legend: "A - F' Upper panels (A - F) show morphology of c4da neurons of the indicated genotypes at the third instar larval stage, lower panels (A' - F') show c4da neuron morphology at 18 h after puparium formation (APF). Neurons were labeled by CD8::GFP expression under ppk-GAL4 or by tdtomato expression in MARCM clones. (A, A') C4da neurons expressing control Orco dsRNA. Scale bars are 100 μm in A (for larval images) and 50 μm in A' (for pupal images). (B, B') C4da neurons expressing PP2A-29B dsRNA. (C, C') Homozygous PP2A-29BGE16781 mutant c4da neuron MARCM clones. (D, D') Homozygous PP2A-29BGE16781 mutant c4da neuron MARCM clones expressing UAS-HAPP2A-29B. (E, E') C4da neurons expressing dominant-negative Mts. (F, F') Homozygous mtss5286 mutant c4da neuron MARCM clones. G Penetrance of pruning defects at 18 h APF in A' - E'. ** P&lt;0.005, *** P&lt;0.0005, Fisher's exact test. Number of neurons for each genotype are given in the figure, the number of animals is given below in parentheses. H Severity of pruning defects at 18 h APF in A' - E' as assessed by number of primary and secondary dendrites attached to soma at 18 h APF. Data are mean +/- s. d., ** P&lt;0.005, *** P&lt;0.0005, Wilcoxon's test. Numbers of neurons (animals) for each genotype are given in the figure. "
NCBI gene IDs:
"HA: 
tdtomato: 
GAL4: 855828
Mts: 45959
mts: 45959
Orco: 40650
PP2A-29B: 2768940
ppk: 34843"
Figure legend: "A-J The indicated Sox14 or Mical constructs were tested for their ability to suppress pruning defects induced by PP2A-29B dsRNA expression. C4da neurons were labeled by CD8::GFP expression under ppk-GAL4, and dendrite pruning defects were assessed at 18 h APF. Panels A - E show effects of Sox14/Mical pathway manipulations alone, panels F - J show neurons coexpressing PP2A-29B dsRNA. The scale bar in A is 50 μm. (A, F) C4da neurons (co-)expressing lacZ. (B, G) C4da neurons (co-)overexpressing Sox14. (C, H) C4da neurons (co-)overexpressing Mical. (D, I) C4da neurons (co-)expressing Mical∆redox. (E, J) C4da neurons (co-)expressing Mical∆CH. K Penetrance of pruning defects in panels A - J. * P&lt;0.05, *** P&lt;0.0005, Fisher's exact test. Numbers of neurons (animals) for each genotype are given in the figure the numbers of animals are given below in parentheses. L Severity of pruning defects in panels A - J as assessed by number of primary and secondary dendrites attached to soma at 18 h APF. Data are mean +/- s. d., * P&lt;0.05, *** P&lt;0.0005, Wilcoxon's test. Numbers of neurons (animals) for each genotype are given in the figure. "
NCBI gene IDs:
"GAL4: 855828
lacZ: 945006
Mical: 41225
PP2A-29B: 2768940
ppk: 34843
Sox14: 37822"