EMBO/SourceData huggingface dataset:
Figure legend: "EUC1 displays negative genetic interactions with genes involved in particular with members of the Rpd3L histone deacetylase complex For , serial dilutions of the indicated strains were spotted and grown on YPD control or selective media plates, or under conditions as indicated. Note that all strains used in (A-D) contained an extra copy of MED11,"
NCBI gene IDs:
"MED11: 855139
EUC1: 855138"
Figure legend: "For serial dilutions of the indicated strains were spotted and grown on YPD control or selective media plates, or under conditions as indicated. Note that all strains used contained an extra copy of MED11 (C) Plasmid-borne EUC1 complements genetic interactions with Rpd3L subunits. Empty vector (-) or a plasmid encoding EUC1 with its endogenous promoter were transformed in the indicated strains and spotted on selective media."
NCBI gene IDs:
"MED11: 855139
EUC1: 855138"
Figure legend: "For serial dilutions of the indicated strains were spotted and grown on YPD control or selective media plates, or under conditions as indicated. Note that all strains used contained an extra copy of MED11 (D) EUC1 and Rpd3S act in a common pathway which is redundant with Rpd3L. rco1∆ and eaf3∆ (Rpd3S) show similar phenotypes when paired with sds3∆ (Rpd3L) as euc1∆ and are epistatic with euc1∆."
NCBI gene IDs:
"eaf3: 856134
MED11: 855139
rco1: 855097
sds3: 854725
EUC1: 855138
euc1: 855138"
Figure legend: "The ability to form ub-hotspots is crucial for endogenous EUC1 function. The genetic interaction of EUC1 with SDS3 (Rpd3L complex) was rescued with plasmid-borne EUC1-alleles and serial dilutions were spotted on selective media. (-) denotes empty vector."
NCBI gene IDs:
"SDS3: 854725
EUC1: 855138"
Figure legend: "SLX5 also shows negative genetic interactions with SDS3 upon heat stress. Serial dilutions of the indicated strains were spotted on YPD and incubated as indicated. See also Appendix Fig. S12C-D. Note that all strains used in (A-C) contained an extra copy of MED11,"
NCBI gene IDs:
"MED11: 855139
SDS3: 854725
SLX5: 851549"
Figure legend: "C-E. Western blot analysis for CCNA, CCNB, Cdk1 and GAPDH (loading control) protein expression in lysates from control (si-GFP) and p53-depleted (si-p53; si-p53_1) (C) MDA-MB-231 and CAL27 cell lines. Representative images are shown."
NCBI gene IDs:
"p53: 7157"
Figure legend: "C-E. Western blot analysis for CCNA, CCNB, Cdk1 and GAPDH (loading control) protein expression in lysates from control (si-GFP) and YAP-depleted (si-YAP; si-YAP_1) (D) MDA-MB-231 and CAL27 cell lines. Representative images are shown."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "C-E. Western blot analysis for CCNA, CCNB, Cdk1 and GAPDH (loading control) protein expression in lysates from control (si-GFP) and TAZ-depleted (si-TAZ) (E) MDA-MB-231 and CAL27 cell lines. Representative images are shown."
NCBI gene IDs:
"TAZ: 6901"
Figure legend: "A. Primary human breast cancers of the METABRIC dataset were stratified according to high or low YAP activity signature [47] and by TP53 mutational status, and then the levels of the cycline signature score were determined in the four groups. Cyclin activity is significantly higher in mut-p53 tumors with high levels of the YAP signature, as visualized by box-plot. Signature scores have been obtained summarizing the standardized expression levels of signature genes into a combined score with zero mean [7]. The values shown in graphs are thus adimensional. The bottom and top of the box are the first and third quartiles, and the band inside the box is the median; whiskers represent 1st and 99th percentiles; values lower and greater are shown as circles (p&lt;0.0001, n=701)."
NCBI gene IDs:
"p53: 7157
TP53: 7157
YAP: 10413"
Figure legend: "B. Same as in A) using the YAP/TAZ activity signature of Zhang et al. (2009) (p&lt;0.0001, n=701)."
NCBI gene IDs:
"TAZ: 6901
YAP: 10413"
Figure legend: "C. Kaplan-Meier analysis representing the probability of disease-specific survival in mutant and wild-type p53 breast cancer patients from the METABRIC dataset stratified according high or low YAP/TAZ signature score. The log-rank test p-value reflects the significance of the association between high levels of the YAP/TAZ signature score and shorter survival in in mutant-p53 as compared to wild-type p53 patients (p&lt;0.0001, n=251). All p-values were calculated with two-tailed Student's t-test."
NCBI gene IDs:
"TAZ: 6901
p53: 7157
YAP: 10413"
Figure legend: "A. SKBr3 and MDA-MB-468 cells knocked-down as indicated in the figure, were transiently transfected with pCCAAT-B2LUC (100 ng) or pmutCCAAT-B2LUC (100 ng) luciferase reporter vectors. Values are means + s.d. of three replicates from three independent experiments."
NCBI gene IDs:
"LUC: "
Figure legend: "B. RT-qPCR of CCNA, CCNB2 and cdk1 mRNA levels in SKBr3 cells upon transduction with si-GFP and si-YAP (left graph) or si-p53 (right graph). Values are means + s.d. of three replicates from three independent experiments."
NCBI gene IDs:
"GFP: 
CCNA: 8900///890
CCNB2: 9133
cdk1: 983
p53: 7157
YAP: 10413"
Figure legend: "C. H1299 transfected with the indicated siRNAs, were transiently transfected with pCCAAT-B2LUC (100 ng) luciferase reporter vector together with empty pcDNA3 or mutant p53R175H. Representative western blotting to control the transfections presented in the right panel. Values are means + s.d. of three replicates from three independent experiments."
NCBI gene IDs:
"LUC: 
p53: 7157"
Figure legend: "D. RT-qPCR of CCNA and cdk1 mRNA levels in H1299 cells upon transfection with si-GFP or si-YAP oligos and empty pCDNA3 or mutp53R280H expression vectors as indicated in the figure. Error bars represent mean + s.d., from three biological replicates. P-values are indicated in the figures; two-tailed Student's t-test was applied throughout."
NCBI gene IDs:
"GFP: 
CCNA: 8900///890
cdk1: 983
p53: 7157
YAP: 10413"
Figure legend: "A and B. Coimmunoprecipitation and western blot analysis from H1299 cell lysates showing endogenous YAP (A) and overexpressed GFP-YAP (B) bound to mutp53R273H (A) or to mutp53R175H (B). In (A) YAP protein was immunoprecipitated with a rabbit polyclonal antibody and rabbit IgG was used as negative control of IP. In (B) p53 protein was immunoprecipitated with a sheep polyclonal antibody and the total lysate from H1299 transfected with empty pCDNA3 vector was used in as negative control of IP."
NCBI gene IDs:
"p53: 7157
YAP: 10413"
Figure legend: "C and D. Immunoprecipitations of YAP protein and western blot analysis for p53 binding are performed from lysates of cancer cell lines expressing different mutant p53 proteins (described in Materials and Methods) (C) YAP protein was immunoprecipitated with a rabbit polyclonal antibody and the same amount of rabbit IgG was used as negative control of IP."
NCBI gene IDs:
"p53: 7157"
Figure legend: "G. Cell lysates from CAL27 cells transfected with si-GFP and si-NF-YB oligonucleotides were immunoprecipitated with rabbit polyclonal YAP. As specificity control, immunoprecipitations were performed with rabbit IgG."
NCBI gene IDs:
"GFP: 
NF-YB: 4801"
Figure legend: "H and I. ChIP analysis of mutant p53, YAP and H4 acetylated histone-bound chromatin from MDA-MB-468 cells on transduction with siRNAs oligos targeting endogenous YAP (siYAP) (H), NF-Y (siNF-YB) (I), or siGFP as a negative control (H and I). The experiment was performed in biological triplicates. The CCNA, CCNB2 and Cdk1 promoter occupancy was analyzed by RT-qPCR. Normalization was performed to the amount of input chromatin. The ChIP results were further normalised on the RT-qPCR of a region resulted negative for the recruitment of mutant p53 (Appendix Table S5). P-values were calculated with two tailed Student's t-test. Statistically significant results were referred with p-value&lt;0.05."
NCBI gene IDs:
"GFP: 
CCNA: 8900///890
CCNB2: 9133
Cdk1: 983
NF-YB: 4801
YAP: 10413"
Figure legend: "A and B. Proliferation curves of SKBr3 cell line knocked-down for YAP (A) or p53 (B). si-GFP oligos are used as negative control.Cell viability analysis in A and B was determined by trypan blue dye exclusion staining, in C was determined by ATPlite luminescence analysis. All the values are means + s.d. of six replicates from three independent experiments."
NCBI gene IDs:
"GFP: 
p53: 7157
YAP: 10413"
Figure legend: "C. Viability of CAL27 cell line transfected with si-GFP or si-p53 oligos and empty vector or GFP-YAP construct, as indicated in the legend to the graph.Cell viability analysis in A and B was determined by trypan blue dye exclusion staining, in C was determined by ATPlite luminescence analysis. All the values are means + s.d. of six replicates from three independent experiments."
NCBI gene IDs:
"GFP: 
p53: 7157
YAP: 10413"
Figure legend: "A. CCNA and CCNB2 expression transcripts were analyzed by RT-qPCR in cDNA derived from tumours of control (saline) or zoledronic acid (ZA)-treated mice (ref. 31). N=3 mice per group. P-values were calculated with two-tailed t-test."
NCBI gene IDs:
"CCNA: 12427///12428
CCNB2: 12442"
Figure legend: "B. ChIP analysis of mutant p53 -bound chromatin from MDA-MB-231 cells treated or not with 1 µM cerivastatin (Cer) for 24h. The CCNB2 promoter occupancy was analyzed by RT-qPCR. Normalization was performed to the amount of input chromatin. The experiment was performed in biological triplicate. P-values were calculated with two-tailed t-test."
NCBI gene IDs:
"CCNB2: 9133"
Figure legend: "C. MDA-MB-231 cells treated with the indicated concentrations of Cer for 24h were transfected with pCCAAT-B2LUC (100 ng) luciferase reporter vector. Error bars represent mean + s.d., from three biological replicates. P-values are indicated in the figures;p-values were calculated with two-tailed t-test."
NCBI gene IDs:
"LUC: "
Figure legend: "D. CCNA, CCNB2 and Cdk1 expression transcripts were analyzed by RT-qPCR in cDNA derived from MDA-MB-231 (left panel) and SKBr3 (right panel) cell lines treated or not with 1 µM Cer for 48h. Error bars represent mean + s.d., from three biological replicates. P-values were calculated with two-tailed t-test. P-value&lt;0.01."
NCBI gene IDs:
"CCNA: 8900///890
CCNB2: 9133
Cdk1: 983"
Figure legend: "F. CCNA, CCNB2 and Cdk1 expression transcripts were analyzed by RT-qPCR in cDNA derived from MDA-MB-231cell line treated or not with 30µM ZA for 48h. Error bars represent mean + s.d., from three biological replicates. P-values were calculated with two-tailed t-test. P-value&lt;0.01."
NCBI gene IDs:
"CCNA: 8900///890
CCNB2: 9133
Cdk1: 983"
Figure legend: "G. Viability assay of siGFP and sip53 -MDA-MB-231 cells after treatment with increasing amounts of cerivastatin (0, 0.01, 0.1, 1 and 10 µM) for 48 h. Data are normalized to untreated. Error bars represent mean + s.d., from three biological replicates."
NCBI gene IDs:
"GFP: 
p53: 7157"
Figure legend: "(D and E) Growth comparisons of bir1∆-ad2 strains that contain (18 strains) or do not contain (28 strains) confirmed suppressor mutations as measured by area of colony growth after serial dilution. The mean (red line) is shown. 10-fold serial dilutions were made on YPAD plates containing either 0.1% DMSO (D) or 10µg/mL of benomyl (E)."
NCBI gene IDs:
"bir1: 853551
ad2: 853958"
Figure legend: "(A) Serial dilutions on YPAD media at the permissive (25˚C) or restrictive (35˚C) temperatures for the ipl1-321 mutation. The suppressor mutations from the Dam1c (blue) were the only mutants that rescued viability at the restrictive temperature."
NCBI gene IDs:
"ipl1: 855892"
Figure legend: "(B) Serial dilutions of strains engineered with the indicated mutations were tested for rescue of BIR1 deletion. 10-fold serial dilutions on the indicated media are shown. dam1(S20D) rescues loss of CPC activity to a similar extent as a suppressor mutant in Duo1."
NCBI gene IDs:
"BIR1: 853551
dam1: 853010
Duo1: 852819"
Figure legend: "(A) Serial dilutions of strains engineered with the indicated mutations were tested for rescue of BIR1 deletion. 10-fold serial dilutions on the indicated media are shown. The met30-6 and cdc4-1 alleles are temperature sensitive. Two independent clones of each of these alleles are shown. The serial dilutions were performed at the permissive temperature (20˚C)."
NCBI gene IDs:
"BIR1: 853551
cdc4: 850539
met30: 854765"
Figure legend: "(B) The amount of mNeonGreen-Sli15 (CPC member) located at kinetochores (Nuf2-mCherry) was measured in prometaphase. Sgo1 was depleted by placing it under a galactose-inducible promoter and switching to glucose-containing media (YPAD). Representative images are on the left. Scale bar is 2µm. Averages from 3 independent experiments are shown."
NCBI gene IDs:
"Sgo1: 854240"
Figure legend: "G) Quantification of the percentage of CD11b+CD45low microglia  containing  lipid droplets as Bodipy+ microglia  using flow cytometry in male mice  deficient in Stat1  (Stat1-/-) (n=4) and corresponding Stat1+/+ mice  (n=7) shows ischemia-induced increases in Stat1+/+ mice  (** p=0.0086) but not in Stat1-/- mice  (p=0.927) (Two-way ANOVA and Šídák's multiple comparisons test). Graph shows values of the non-ischemic  (-) and ischemic  (+) brain  hemispheres of individual mice  and the mean±SD."
NCBI gene IDs:
"Stat1: 20846"
Figure legend: "A) Representative zooms of WT MEFs transfected with GFP-tagged Miro1 and Miro2 (GFPMiro1 and GFPMiro2). Control is GFP fused to the first 70 amino acids of Tom70. Tom20 and catalase stain mitochondria and peroxisomes, respectively. Merge is of Miro (green) and catalase (magenta) with colocalisation shown as white. White arrowheads highlight an area where an isolated peroxisome is situated. B) Quantification of the extent of GFP signal on peroxisomes. Data information: B) One-way ANOVA with Newman-Keuls post hoc test was used for all comparisons (n=18 cells per condition over 3 independent experiments). *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001 in comparison to Control, respectively, and ## and ### denote p &lt; 0.01 and p &lt; 0.001 in comparison to GFPMiro1, respectively. Data are represented as mean ± SEM. Scale bar is 5 μm."
NCBI gene IDs:
"GFP: 
Miro1: 55288
Miro2: 89941"
Figure legend: "C) Pulldown of overexpressed Pex19 in Cos7 cells with a Pex19 antibody shows interaction with full-length Miro1, but not Miro1 lacking the transmembrane domain."
NCBI gene IDs:
"Pex19: 19298"
Figure legend: "E) Representative images of Miro1 truncation constructs expressed in DKO MEFs. Mitochondria and peroxisomes are stained with Tom20 and catalase, respectively. White arrowheads highlight an area where an isolated peroxisome is situated.F"
NCBI gene IDs:
"Miro1: 55288"
Figure legend: ".F) Quantification of the extent of peroxisomal localisation of the Miro1 truncation constructs. Data information: F) One-way ANOVA with Newman-Keuls post hoc test was used for all comparisons (n=18 cells per condition over 3 independent experiments). *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001 in comparison to Control, respectively, and ## and ### denote p &lt; 0.01 and p &lt; 0.001 in comparison to GFPMiro1, respectively. Data are represented as mean ± SEM. Scale bar is 5 μm."
NCBI gene IDs:
"Miro1: 55288"
Figure legend: "G) Co-immunoprecipation of Miro1 truncation constructs and mycPex19 following transfection into Cos7 cells. GFP-tagged Miro1 truncation constructs were pulled down with GFP-Trap agarose beads and Pex19 was probed with myc antibody."
NCBI gene IDs:
"myc: 
Pex19: 19298
Miro1: 55288"
Figure legend: "B) Blind scoring of the number of long-range peroxisomal trafficking events in WT, Miro1KO and Miro2KO and DKO MEFs (n=42 cells over six independent experiments. Two different MEFs lines were used for each genotype). Data information: For B) a Kruskall-Wallis with a Dunn's correction posthoc test was used to test for significance. For B) no statistical difference between conditions was observed, unless stated. Data are represented as mean ± SEM."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "E) Representative blots of Miro1, Miro2 and actin from whole cell lysates of wild-type and Miro1-floxed ERT Cre-recombinase MEFs treated with and without 4-OH tamoxifen for 48 hours. Lysates were taken one day after the end of treatment."
NCBI gene IDs:
"Cre-recombinase: 2777477
ERT: 2100
Miro1: 59040"
Figure legend: "G) Representative blot of Miro1 and Miro2 following knockdown of either Miro1 or Pex14 in HeLa cells."
NCBI gene IDs:
"Pex14: 5195
Miro1: 55288"
Figure legend: "H) Quantification of long-range peroxisomal trafficking events (visualised with pxGFP) in HeLa cells transfected with scrambled, Miro1 and Pex14 siRNA, by blind scoring of a five-minute movie (n=18 cells over three independent experiments). Data information: For H) is a one-way ANOVA with Newman-Keuls post-hoc test. ** denotes p &lt; 0.01. For H), no statistical difference between conditions was observed, unless stated. Data are represented as mean ± SEM."
NCBI gene IDs:
"Pex14: 5195
Miro1: 55288"
Figure legend: "A) Representative images of WT, Miro1KO, Miro2KO and DKO MEFs seeded onto Y-shaped fibronectin micropatterns stained for mitochondria (Tom20 in red) and peroxisomes (Catalase in white). Scale bar represents 10 μm. Data information: n = 60 cells over three independent experiments."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "B) Normalised cumulative distribution of mitochondria in WT, Miro1KO, Miro2KO and DKO MEFs. C) Distance at which 95% of mitochondria are distributed (Mito95) for all four genotypes of MEF. Data information: n = 60 cells over three independent experiments. For C) one-way ANOVA with Newman-Keuls post-hoc test was used to test for statistical significance. *** represents p &lt; 0.001. Data are represented as mean ± SEM."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "D) Normalised cumulative distribution curves comparing the peroxisomal distribution from the centre of the cell to the periphery of WT, Miro1KO, Miro2KO and DKO MEFs. E) Bar graph comparing the average distance at which 95% of the peroxisomal signal is distributed (Perox95). Data information: n = 60 cells over three independent experiments. For E) a one-way ANOVA with Newman-Keuls post-hoc test was used to test for statistical significance. *** represents p &lt; 0.001. Data are represented as mean ± SEM."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "A) Snapshots and tracks of pxDsRed signal in WT, Miro1KO, Miro2KO and DKO MEFs following live imaging at 1.5 seconds per frame for two minutes. B) Quantification of median net displacement of individual peroxisomes (pxDsRed signal) for WT, Miro1KO, Miro2KO and DKO MEFs (n=42 cells over six independent experiments. Two different MEFs lines were used for each genotype). Data information: For multiple comparisons in B) a one-way ANOVA with Newman-Keuls post-hoc test was used to test for statistical significance."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "F) Median net displacement of peroxisomes in DKO MEFs with and without myc-tagged variant 4 of Miro1 (mycv4) (n=12 cells per condition over three independent experiments). Data information: Student's t-test was used to calculate statistical significance. * and *** represent p&lt;0.05 and p&lt;0.001, respectively. Data are represented as mean ± SEM."
NCBI gene IDs:
"myc: 
Miro1: 59040
v4: 59040"
Figure legend: "A) Representative images of catalase staining (peroxisomes) in WT, Miro1KO, Miro2KO and DKO MEFs."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "B) Bar graph comparing average peroxisomal area of WT Miro1KO, Miro2KO and DKO MEFs ( n = 60 cells per condition over three independent experiments). Data information: Calculation of statistical significance in B) was a one-way ANOVA with Newman-Keuls post-hoc. *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001, respectively. Data are represented as mean ± SEM. Scale bar is 10 μm. Scale bar in zooms is 5 μm."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "C) Comparison of total peroxisomal number between WT, Miro1KO, Miro2KO and DKO MEFs. n = 60 cells per condition over three independent experiments. Data information: Calculation of statistical significance in C) was a one-way ANOVA with Newman-Keuls post-hoc. *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001, respectively. Data are represented as mean ± SEM. Scale bar is 10 μm. Scale bar in zooms is 5 μm."
NCBI gene IDs:
"Miro1: 59040
Miro2: 214952"
Figure legend: "D) Representative images of Control (GFP-tagged 1-70 of Tom70), GFPMiro1 and GFPMiro2 in WT MEFs stained with catalase (peroxisomes)."
NCBI gene IDs:
"GFP: 
Miro1: 59040
Miro2: 214952"
Figure legend: "E) Representative images of Pex14 (peroxisomes) in DKO MEFs expressing either GFP (control) or GFP-tagged variant 4 of Miro1 (GFPv4)."
NCBI gene IDs:
"GFP: 
Miro1: 59040
v4: 59040"
Figure legend: "F) Bar graph comparing average peroxisomal area of Control, GFPMiro1 and GFPMiro2 overexpressing WT MEFs (n=60 cells per condition over three independent experiments). Data information: Calculation of statistical significance in F) was a one-way ANOVA with Newman-Keuls post-hoc. *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001, respectively. Data are represented as mean ± SEM. Scale bar is 10 μm. Scale bar in zooms is 5 μm."
NCBI gene IDs:
"GFP: 
Miro1: 55288
Miro2: 89941"
Figure legend: "G) Comparison of average area of individual peroxisomes between DKO MEFs expressing either GFP (control) or GFP-tagged variant 4 of Miro1 (GFPv4) (n=30 cells per condition over three independent experiments). Data information: Statistical test used in G) was a two-tailed Student's t test. *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001, respectively. Data are represented as mean ± SEM. Scale bar is 10 μm. Scale bar in zooms is 5 μm."
NCBI gene IDs:
"GFP: 
Miro1: 59040
v4: 59040"
Figure legend: "C) Representative images of a Fis1-Drp1 PLA in DKO MEFs expressing either GFP, GFPv1 (GFP-tagged variant 1 of Miro1) or GFPv4 (GFP-tagged variant 4 of Miro1) n=30 cells per condition over three independent experiments). Scale bar is 10 μm. Red dots indicate interaction of Fis1 and Drp1. Blue is DAPI. D) Quantification of PLA fluorescent dots per cells between GFP, GFPv1 or GFPv4 transfected DKO MEFs (n=30 cells over three independent experiments). Data information: In D) a one-way ANOVA with a Newman-Keuls post-hoc test was used to calculate statistical significance. *, ** and *** denote p &lt; 0.05, p &lt; 0.01 and p &lt; 0.001, respectively. Data are represented as mean ± SEM."
NCBI gene IDs:
"GFP: 
Miro1: 59040
v1: 59040
v4: 59040"
Figure legend: "B) Western blot analysis of HEL whole cell lysate after transfection with cDNA coding for JAK2WT or JAK2V617F, alone or cotransfected with cDNA coding for Mpl."
NCBI gene IDs:
"JAK2: 3717"
Figure legend: "C) Summary of the ratios of mature:immature Mpl for at least three independent (2 for the JAK2V617F transfection) blotting experiments under the protocol shown in (B). Based on two−tailed Student t−test statistical analysis, there is a significant difference (p = 0.0001) in the ratio of Mpl isoforms in cells transfected with JAK2WT by comparison with control cells. Differences were also statistically significant in HEL cells transfected with either JAK2V617F or JAK2L611V/V617F by comparison to controls (p = 0.0159 and p = 0.0009, respectively). The mature:immature ratio is also significantly higher in cells transfected with JAK2WT than in cells transfected with JAK2V617F or JAK2L611V/V617F (p &lt; 0.01)."
NCBI gene IDs:
"JAK2: 3717"
Figure legend: "D) Western blot analysis of K562 lysates after transfection with cDNA coding for Mpl, alone or cotransfected with cDNA coding for JAK2WT or JAK2V617F."
NCBI gene IDs:
"JAK2: 3717
Mpl: 4352"
Figure legend: "Confocal fluorescent images of HEL cells (JAK2V617F+/+) after immuno‐fluorescence labeling of endogenous Mpl (A), or after transient transfection with cDNA coding for a chimeric Mpl protein fused to mOrange2 (B)."
NCBI gene IDs:
"JAK2: 3717
Mpl: 4352"
Figure legend: "C) Confocal fluorescence image of K562 cells (JAK2WT+/+) transiently transfected with cDNA coding for MplmOrange2. Red arrow in this image points to plasma membrane localization."
NCBI gene IDs:
"JAK2: 3717"