EMBO/SourceData huggingface dataset:
Figure legend: "E Left: x-ray  3D images obtained from mice  8 weeks after intracardiac injections with control (PC-3 Luc) and cells stably overexpressing NALCN  (+hNALCN). Right: box plots compare the incidence and total volume of bone lesions between the two groups of 11 mice: central band shows the mean, the boxes show lower and upper quartiles and whiskers show maximum data values. Data information: Red numbers and/or arrows in the images depict tumour take incidence/bone lesions. *P&lt;0.05, **P&lt;0.01, and n.s., not significant, two-tailed Mann-Whitney U test."
NCBI gene IDs:
"Luc: 
NALCN: 259232"
Figure legend: "B) Western blots showing absence of mitochondrial proteins from the different mitochondrial complexes: NDUFB8 (complex I), SDHA (complex II), UQCRC2 (complex III) and MTCO-1 (complex IV), in senescent (10 days after 20Gy X-ray) Control and Parkin-expressing MRC5 fibroblasts. Data is representative of 3 independent experiments;"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "C) Cellular oxygen consumption rates (OCR) in senescent (10 days after 20Gy X-ray) Control and Parkin-expressing MRC5 fibroblasts. Data were obtained using Seahorse XF24 analyzer and shows mean±S.D. n=4 technical repeats (representative of 2 independent experiments);"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "D) Representative 3D EM pictures of senescent (20 days after 20Gy X-ray) Parkin-expressing MRC5 fibroblasts with or without CCCP. Mitochondria are in red, the nucleus in blue;"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "E) Representative images of cell size, Sen-β-Gal activity (blue cytoplasmic staining), macro-H2A foci and SDHA immunofluorescence in proliferating and senescent (10 days after 20Gy X-ray) Parkin- expressing MRC5 fibroblasts;"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "F) Quantification of ROS levels (DHE fluorescence), Sen-β-Gal positive cells and Senescence-associated heterochromatin foci (SAHF) observed by DAPI in proliferating and senescent (10 days after 20Gy X-ray) Control and Parkin-expressing MRC5 fibroblasts. Data are mean±S.E.M of n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "G) Representative western blots showing p21 and p16 expression in proliferating and senescent (10 days after 20Gy X-ray) Control and Parkin-expressing MRC5 fibroblasts. Data are representative of 3 independent experiments;"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "H) Secreted protein measured in a cytokine array (RayBiotech) of a variety of inflammatory proteins in proliferating and senescent (10 days after 20Gy X-ray) Parkin-expressing MRC5 fibroblasts. Data are mean±S.E.M of n=3 independent experiments. Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "A) Representative images of colony assays of wild-type and PGC-1β -/- MEFs grown at 3% or 21% O2 (10 days after seeding 5,000 cells/well). Data are representative of 3 independent experiments;"
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "B) Effect of 3% or 21% O2 and X-ray irradiation (at 3% O2) on the percentage of Ki67 (at day 6) and Sen-β-Gal positive cells (at day 10) and number (N) of 53BP1 foci (at day 6) in wild-type and PGC-1β -/- MEFs. Data are mean±S.E.M of n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "C) Representative images of Sen-β-Gal activity (blue cytoplasmatic staining), Ki-67 and 53BP1 foci in proliferating and senescent wild-type and PGC-1β-/- MEFs (scale bar=10µm);"
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "D) mRNA expression of PGC-1β CXCL-1 and p16 in proliferating and senescent (10 days after 10Gy X-ray) wild type and PGC-1β-/- MEFs. Data are mean±S.E.M. of n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"p16: 12578
CXCL-1: 14825
PGC-1β: 170826"
Figure legend: "E) Effects of overexpression of PGC-1β on the percentage of Ki67 and Sen-β-Gal positive cells, number (N) of 53BP1 foci and percentage change of mitochondrial mass (NAO intensity) in proliferating and senescent (2 days after 10Gy X-ray) MEFs cultured at 3% O2. Data are mean±S.E.M of n=3 independent experiments. Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "C) Effect of 100nM rapamycin on mitochondrial mass (measured by NAO fluorescence) 2-4 days following replication exhaustion (RS) or genotoxic stress (generated by X-ray irradiation, Etoposide, Neocarcinostatin (NCS), H2O2 or telomere dysfunction (TRF2ΔBΔM)) in a variety of cell lines. Data are mean from 3 independent experiments per cell line or treatment;"
NCBI gene IDs:
"TRF2: 7014"
Figure legend: "A) ROS levels (DHE intensity) and mean number (N) of yH2A.X foci after mTOR knockdown (72 hours) in proliferating and senescent (2 days after 20Gy X-ray) MRC5 fibroblasts. Data are mean±S.E.M of n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"mTOR: 2475"
Figure legend: "D) ROS levels (DHE intensity) in proliferating and senescent (3 days after 10Gy X-ray) wild-type and PGC-1β-/- MEFs (top) and PGC-1β overexpressing MEFs (bottom). Data are mean±S.E.M. of n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "E) (top) Representative images and quantification of immunofluorescence staining against FLAG-PGC-1β (red) and 53BP1 (green) in proliferating and senescent (2 days after 10Gy X-ray) PGC-1β overexpressing MEFs cultured at 3% O2, with or without 250µM of the antioxidant Trolox (scale bar=10µm). Data are mean±S.E.M, n=3 independent experiments; Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"PGC-1β: 170826"
Figure legend: "F) (top) Effect of overexpression of mutated Rheb (N153T) and (bottom) effect of 100nM rapamycin on the mean number (N) of 53BP1 foci in proliferating and senescent (3 days after 10Gy X-ray) wild-type and PGC-1β -/- MEFs. Data are mean±S.E.M of n=3 independent experiments (at least 125 cells were analyzed per condition). Asterisks denote statistical significant P&lt;0.05 One-way ANOVA."
NCBI gene IDs:
"PGC-1β: 170826
Rheb: 19744"
Figure legend: "j) Effect of 4 months rapamycin feeding on mRNA expression of the SASP components CXCL1, CXCL5 and Inhibin A in liver tissue of 16 months old mice with or without 4 months rapamycin treatment. Data are from n=5 mice per condition; Asterisks denote statistical significant P&lt;0.05 using Two-tailed t-test."
NCBI gene IDs:
"CXCL1: 14825
CXCL5: 20311
Inhibin A: 16322"
Figure legend: "K) Mean number of TAF in hepatocytes of wild type and PGC-1-β-/- mice with 18 months of age. Data are mean±S.E.M of n=4 mice per group; Asterisks denote statistical significant P&lt;0.05 using Two-tailed t-test."
NCBI gene IDs:
"PGC-1-β: 170826"
Figure legend: "A. Eight-day-old seedlings of WT, bri1 and pRPS5a:BRI1:YFP;bri1. Scale bar 1 cm."
NCBI gene IDs:
"bri1: 830095"
Figure legend: "B. Root-length measurements of 10-day-old bri1-116, bes1-D and pRP5SA:BRI:YFP;bri1-116 seedlings compared to the wild type. Symbols represent the mean of more than 20 plants, from 3 independent experiments. Bars show standard deviation. Straight lines represent the linear regression applied to each curve starting with day 4 post germination. In this way the root growth rate can be extracted for each phenotype (See Table EV7)."
NCBI gene IDs:
"BRI: 830095
bri1: 830095"
Figure legend: "C. Confocal images of 8-day-old wild type WT, bri1-116 and pRP5SA:BRI:YFP;bri1-116 roots stained with PI. Green lines label the end of the transition zone and the yellow lines label the first root hair (end of elongation zone). The inset shows pRP5SA expression domain of an 8-day-old pRP5SA:BRI:YFP;bri1-116 seedling. Green line labels the end of the meristematic zone, which coincides with the end of pRP5SA expression domain. Scale bars correspond to 100 microns."
NCBI gene IDs:
"bri1: 830095"
Figure legend: "C-H. Main relationships as in Fig. 2C-E between experimental values of the phenotypic traits used to assess the mechanism of differentiation in bri1-116 mutant (C,E,G) and pRP5SA:BRI:YFP;bri1-116 line (D,F,H) for the epidermis (left large plot) and cortex (right inset plot). Each circle denotes data extracted from a single root (n=126 (epidermis) and n=25 (cortex) for bri1-116 and n=90 (epidermis) and n=17 (cortex) for pRP5SA:BRI:YFP;bri1-116, see Table EV1, pooled from 6, 8 and 10 day-old seedlings for epidermis and from day 6 for cortex (data in Table EV5)). In G, H, the slope of the linear regression is 2.13±0.04 for bri1-116 and 2.53±0.04 for pRP5SA:BRI:YFP;bri1-116. Pearson correlation coefficient r is indicated and p-value (p, using standard Person correlation test). In E, bri1-116 epidermal data do not conform to normal distributions."
NCBI gene IDs:
"bri1: 830095"
Figure legend: "(c) Knockdown of LC3 or Beclin 1 potentiated Wnt signalling. (n = 3; asterisk, P 0.05)."
NCBI gene IDs:
"Beclin 1: 8678
LC3: 440738///81631///84557"
Figure legend: "(d) Atg5 knockout enhanced Wnt signalling. TopFlash reporter activity induced by Wnt3a CM in Atg5−/− or wild-type MEFs. Results are means ± s.d. after normalization to Renilla luciferase activity. (n = 3; asterisk, P 0.05)."
NCBI gene IDs:
"Wnt: 
Atg5: 11793"
Figure legend: "(b) The half-life of Dvl2 was prolonged in Atg5−/− cells. MEFs were subjected to starvation (starv.) for the indicated durations and harvested for immunoblotting with the indicated antibodies. Dvl2 levels were quantified (right panel). Autophagy induction is indicated by the levels of LC3 II and p62 degradation."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(c) Reintroduction of Atg5 in Atg5−/− MEFs restored starvation-induced Dvl2 degradation. MEFs infected with recombinant adenovirus expressing GFP or Atg5 were starved for the indicated durations, and the cell lysates were subjected to immunoblotting with the indicated antibodies."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(d) Dvl2 degradation was slowed down in Atg7−/− MEFs. MEFs were subjected to starvation for the indicated durations and harvested for immunoblotting with the indicated antibodies. Dvl2 levels were quantified (right panel)."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "(e) The continued degradation of Dvl2 in Atg5−/− cells is proteasome-dependent. MEFs were starved for 2, 4 or 8 h with MG132 (1 μM) or BFA1 (0.1 μM) for different durations, followed by anti-Dvl2 immunoblotting to detect endogenous Dvl2 protein levels. Tubulin served as a loading control. Uncropped images of blots are shown in Supplementary Information, Fig. S8."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(a) Immunoelectron microscopy analysis of HeLa cells transfected with Flag-Dvl2 under nutrient-rich or starvation conditions. The arrowheads indicate Flag-Dvl2. Scale bars, 1 μm (left); 250 nm (right)."
NCBI gene IDs:
"Dvl2: 1856"
Figure legend: "(b) Localization of endogenous Dvl proteins (red) and GFP-LC3 (green) in GFP-LC3 stable HeLa cells in nutrient-rich medium or under starvation (starv.) conditions with BFA1 for 4 h. The right panel shows the percentage co-localization between Dvls and GFP-LC3-positive dots. Scale bar, 5 μm."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "(g) The LC-3-binding motif is important for the Dvl2-LC3 interaction. GST-LC3-immobilized beads were incubated with the lysates from the cells expressing wild-type (WT) Dvl2 or the W444A, I447A mutant (MUT); the pulldowns were then subjected to anti-haemagglutinin (anti-HA) immunoblotting. Uncropped images of blots are shown in Supplementary Information, Fig. S8."
NCBI gene IDs:
"Dvl2: 1856"
Figure legend: "(a) Interaction between LC3 and wild-type Dvl2 or its point mutants."
NCBI gene IDs:
"Dvl2: 1856"
Figure legend: "(b) Co-localization of GFP-LC3, p62 and Dvl in HeLa GFP-LC3 stable cells on starvation. Proteins visualized by immunofluorescence with anti-GFP (green), anti-p62 (blue) and anti-Dvl (red) antibodies. Scale bar, 10 μm."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "(c) p62 knockdown slows the degradation of Dvl2 on starvation. HEK-293T cells were transfected with p62 siRNA. At 48 h after transfection, cells were subjected to starvation for various times. The cell lysates were subjected to immunoblotting with the antibodies indicated. Uncropped images of blots are shown in Supplementary Information, Fig. S8."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(b) In vitro ubiquitylation of Dvl2 by the pVHL E3 ligase complex including pVHL, Elongin B, Elongin C, Rbx1 and Cul2. In vitro-translated Flag-Dvl2 was incubated with or without purified E1, E2 and E3 ligase complex, Elongin C and pVHL. The reaction was subjected to anti-Flag immunoprecipitation. Dvl2 ubiquitylation was examined by using anti-ubiquitin immunoblotting."
NCBI gene IDs:
"Elongin C: 6921
VHL: 7428"
Figure legend: "(d) Starvation elevates Dvl2-Cul2 interaction. After co-transfection with Myc-Cul2 and Flag-Dvl2 for 36 h, HEK-293T cells were cultured in nutrient-rich medium or starved with BFA1 (0.1 μM) for 4 h. Cells were harvested for anti-Myc immunoprecipitation followed by anti-Flag immunoblotting. Total protein expression was confirmed by immunoblotting with whole-cell lysates."
NCBI gene IDs:
"Cul2: 8453"
Figure legend: "(a, b) Depletion of Beclin 1 by shRNA promotes growth in soft agar. SW480 cells stably expressing Beclin 1 shRNA or control shRNA were seeded in soft agar and treated with or without Dkk (a); the colonies were then scored after 10 days (b). Numbers in bars indicate the numbers of colonies. Results are means ± s.d. (n = 4). (Asterisk, P 0.05). Scale bar, 200 μm."
NCBI gene IDs:
"Beclin 1: 8678"
Figure legend: "(A) Survival graphs of newborn pups from breeding of Rab11aFl/+;Rab11b+/-;Villin-Cre and Rab11aFl/+;Rab11b+/- (or Rab11aFl/+;Rab11b-/-) mice. The total number of pups for each genotype was labeled next to the corresponding curve. The graph represents over 6 independent litters from three different mating pairs."
NCBI gene IDs:
"Cre: 2777477
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(B) Western blots for Rab11a and Rab11b using small intestinal lysates prepared from adult wild type (WT, lane 1), Rab11aFl/Fl;Villin-CreER (aKO, lane 2) 2 days after tamoxifen treatment, Rab11b-/- (bKO, lane 3), and Rab11aFl/Fl;Rab11b-/-;Villin-CreER (DKO) mice 1-day (lane 4), 2-day (lane 5), and 3-day (lane 6) after tamoxifen treatment. All mice were given a single tamoxifen injection. β-actin was used as loading control. Results represent more than 3 independent experiments."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(C) H. &amp; E. staining of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice, before and after tamoxifen injection. Images represent jejunum tissues collected 1, 2, and 3 days following tamoxifen injection. Experiments were repeated over 5 times using independent litters (n&gt;10). Scale bars, 50 μ"
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(D) Alkaline phosphatase (AP) staining was performed on intestinal tissue sections of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice that were treated with corn oil or with tamoxifen. Results represent at least 3 independent experiments. Scale bars, 50 μm."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(F) Alcian blue staining was performed on adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before or 1 to 3 days after tamoxifen injection. Results represent at least 3 independent experiments. Scale bars, 100 μm. (G) Quantification of the number of alcian blue positive cells per crypt-villus axis in mice of various genotypes. Data represent average values of approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(H) Immunofluorescent staining for lysozyme and E-Cad was performed on adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before and after tamoxifen injection. Experiments represent 3 independent replicates. Scale bars, 50 μm. (I) Quantification of lysozyme fluorescent signal abundance per crypt in mice of various genotypes. Data represent approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(B) Immunohistochemistry for Ki67 staining was performed on intestinal sections of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before and after tamoxifen injection. Experiments represent 3 independent replicates. Scale bars, 50 μm. (C) Quantification of ratio of Ki67+ cells over total epithelial cells per crypt-villus axis in DKO mice before and after tamoxifen treatment. Data represent approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(D) Immunofluorescent staining for pHH3 and E-Cad was performed on intestinal sections of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before and after tamoxifen injection. Experiments represent 3 independent replicates. Scale bars, 50 μm. (E) Quantification of ratio of pHH3+ cells over total IECs per crypt in DKO mice before and after tamoxifen treatment. Data represent approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(H) Immunohistochemistry for Olfm4 was performed on intestinal sections of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before and after tamoxifen injection. Experiments represent 3 independent replicates. Scale bars, 50 μm. (I) Quantification of Olfm4+ cell number per crypt in DKO mice before and after tamoxifen treatment. Data represent approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(L) Immunohistochemistry for c-Myc was performed on intestinal sections of adult Rab11aFl/Fl;Rab11b-/-;Villin-CreER mice before and after tamoxifen injection. Experiments represent 3 independent replicates. Scale bars, 50 μm. (M) Quantification of c-Myc+ cell number per crypt-villus axis in DKO mice before and after tamoxifen treatment. Data represent approximately 10-15 different microscopic fields taken from 3 mice per condition."
NCBI gene IDs:
"Cre: 2777477
ER: 13982
Rab11a: 53869
Rab11b: 19326
Villin: 22349"
Figure legend: "(F) Co-IP assays were performed in HEK293T cells to validate the association between 3×Flag-Rab11a, 3×Flag-Rab11b and endogenous KIF11. Lane 1: input lysate; lane 2: flow through after co-IP; lane 3-5: 3 washes; lane 6: on beads; and lane 7: immunoprecipitation elutes. Cells transfected with 3×Flag empty vector were uses as a negative control. Experiments repeated over 6 times."
NCBI gene IDs:
"Flag: "
Figure legend: "(G) Cells were transfected with mCherry-tagged WT, Rab11-S25N, and Rab11-S20V, fixed and stained for endogenous KIF11 (grey) and pericentrin (green). The mCherry signal (red) represents direct fluorescent signal visualized under confocal microscope. Arrows denote position of spindle poles defined by pericentrin. Images represent three independent experiments. Scale bars, 10 μ (H) Quantification of the percentage of bipolar, monopolar, or multipolar (3 or more) spindles observed in mitotic cells identified from each microscopic field. Data represents 10-20 independent fields per condition from 3 experiments."
NCBI gene IDs:
"mCherry: 
Rab11: "
Figure legend: "(B-C) HEK293T cells were transiently transfected with 3×Flag-Rab11a (or 3×Flag-Rab11b) and V5-tagged KIF11 truncates. Lysates were immunoprecipitated by anti-Flag antibody, and probed by an anti-V5 antibody to assess intracellular associations. Cells transfected by V5 empty vector were used as a negative control."
NCBI gene IDs:
"Flag: 
Rab11a: 
Rab11b: 
V5: 
KIF11: 3832"
Figure legend: "(H) HEK293T cells were transiently transfected with 3×Flag-Rab11a, treated with nocodazole (100 ng/ml) or vehicle overnight, and lysates were used for Flag immunoprecipitation followed by immunoblot for KIF11. Quantification of co-immunoprecipitated KIF11 was normalized to input KIF11 from control (n=6) and nocodazole-treated (n=9) cells."
NCBI gene IDs:
"Flag: 
Rab11a: "
Figure legend: "(L) KIF11 co-IP analysis were performed using WT and RAB11-KD CaCo2 cell lysates. The immunoprecipitates were probed for CLIP1, ZW10, CCAR1, USO1, and CCNB1. Experiments represent 2-4 replicates for each target."
NCBI gene IDs:
"RAB11: 9230///8766"
Figure legend: "Representative apoptotic genes, Anxa1, Anxa5, Bcl2l1 which were highly elevated in DKO intestines. n=3 mice for each genotype."
NCBI gene IDs:
"Anxa1: 16952
Anxa5: 11747
Bcl2l1: 12048"
Figure legend: "(B) In vitro kinase assays showing Munc18-1 phosphorylation by ERK. Radioactive phosphoblot (32P) and immunoblot (IB) of in vitro ERK kinase assay using immunoprecipitatedMunc18WT, Munc184A (M18T78A,S158A,S241A,T574A), Munc183A (M18S158A,S241A,T574A), and Munc18AA (M18S241A,T574A) from HEK293T cells incubated with recombinant ERK2. The phosphoblot shows phosphorylated Munc18-1 (pMunc18-1) and auto-phosphorylated ERK2 (pERK2).(C) Quantification of phospho-Munc18-1 intensity in wild-type and Munc18AA mutant expressed as the amount of 32P incorporation in Munc18-1 normalized to total Munc18-1 levels. Phosphorylation of Munc18WT was set to 100%. (Munc18WT: 100±3%; Munc18AA: 24±2%, n=3, * p = 0.01)."
NCBI gene IDs:
"Munc18: 20910"
Figure legend: "Hippocampal autaptic neurons of munc18-1null mutant mice were rescued with lentiviruses expressing M18WT, M18S241A or M18S241D. Electrophysiology recordings were performed between DIV14-17.(A) Amplitude of excitatory synaptic response (EPSC) (M18WT: 1.37±0.21 nA, n=35; M18S241A: 2.53±0.31 nA, n=45; M18S241D: 0.24±0.05 nA, n=17, ***p&lt;0.001, **p&lt;0.01, *p&lt;0.05)."
NCBI gene IDs:
"munc18-1: 20910"
Figure legend: "Hippocampal autaptic neurons of munc18-1null mutant mice were rescued with lentiviruses expressing M18WT, M18S241A or M18S241D. Electrophysiology recordings were performed between DIV14-17.(B) Percentage of cells that do not respond to electrical stimulation (M18WT: 15.5%; M18S241A: 0%; M18S241D: 56.9%). These cells are not included in (A). Insert: representative recording of single EPSCs. Scale bar represents 10 ms and 0.5 nA."
NCBI gene IDs:
"munc18-1: 20910"
Figure legend: "Hippocampal autaptic neurons of munc18-1null mutant mice were rescued with lentiviruses expressing M18WT, M18S241A or M18S241D. Electrophysiology recordings were performed between DIV14-17.(C) Typical examples of spontaneous vesicle release. Scale bar represents 0.1 s and 25 pA.(D) Spontaneous release frequency (M18WT: 7.05 ± 1.28 Hz, n=32; M18S241A: 14.26 ± 2.04 Hz, n=37; M18S241D: 0.64 ± 0.29 Hz, n=11; ***p&lt;0.001, **p&lt;0.01).(E) Amplitude of spontaneous release events (M18WT: 15.40 ± 1.06 pA, n=17; M18S241A: 15.03 ± 0.61 pA, n=20; M18S241D: 10.98 ± 0.72 pA, n=10; **p&lt;0.01)."
NCBI gene IDs:
"munc18-1: 20910"
Figure legend: "Hippocampal autaptic neurons of munc18-1null mutant mice were rescued with lentiviruses expressing M18WT, M18S241A or M18S241D. Electrophysiology recordings were performed between DIV14-17.(F) Size of the readily releasable pool (RRP) assessed with single hyperosmotic sucrose application (500 mM, 3.5 s, M18WT: 0.74 ± 0.15 nC, n=7; M18S241A: 1.6 ± 0.33 nC, n=10; M18S241D: could not be determined; * p &lt; 0.05)."
NCBI gene IDs:
"munc18-1: 20910"