EMBO/SourceData huggingface dataset:
Figure legend: "G. qPCR analysis of Stat1 and Irf1's expression in the indicated depletion of CT26 cells with/without stimulation of IFNγ. Mean ± SD of n=3. * P &lt; 0.05 by Student's t-tests."
NCBI gene IDs:
"Irf1: 16362
Stat1: 20846"
Figure legend: "I, J. qPCR analysis of the mRNA stability of Stat1 and Irf1 in the indicated CT26 cells treated with IFNγ and actinomycin D. Mean ± SD of n=3. * P &lt; 0.05; * * P &lt; 0.01; * * * P &lt; 0.001 by Student's t-tests."
NCBI gene IDs:
"Irf1: 16362
Stat1: 20846"
Figure legend: "Human osteosarcoma U2OS cells (B or neuroglioma H4 cells (D stably expressing GFP-LC3 were treated with a library of chalcones (30 µM) as indicated , or were left untreated for 6 h. The cells were then fixed and GFP-LC3 dots were counted as an indicator for autophagy. Data are means ± SD (* = p &lt; 0.05; ** = p &lt; 0.01;*** = p &lt; 0.001). Representative images are shown in (B,D). Scale bar equals 10 µm. (C,E) Acetylation: U2OS and H4 cells were treated as described above, followed by incubation with specific antibodies to block acetylated tubulin; Thereafter immunofluorescence was conducted with antibodies against acetylated lysine residues and appropriate AlexaFluor conjugated secondary antibodies before the assessment of cytoplasmic fluorescence intensities. Representative images of acetylation are shown in (C,E). Scale bar equals 10 µm."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "Human osteosarcoma U2OS cells F) or neuroglioma H4 cells H) stably expressing GFP-LC3 were treated with a library of chalcones (30 µM) as indicated or rapamycin (10 µM), or were left untreated for 6 h. The cells were then fixed and GFP-LC3 dots were counted as an indicator for autophagy. Data are means ± SD (* = p &lt; 0.05; ** = p &lt; 0.01;*** = p &lt; 0.001). Viability: U2OS and H4 cells were treated with 30 µM of the indicated chalcones for 24 h and then nuclei were stained with Hoechst 33342 and the number of cells harboring normal nuclei (i.e. non pyknotic, &quot;healthy cells&quot;) was determined. All chalcones were hierarchically clustered upon z-scoring the following phenotypes: autophagy (number of GFP-LC3 dots), viability (number of healthy cells) and acetylation (cytoplasmic fluorescence intensity of acetylated lysine residues) in U2OS and H4 cells. Results are reported as a heatmap (G, I)."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "(G,H) U2OS-GFP-LC3 cells were treated with 30 µM 3,4-DC, 10 µM rapamycin, or left untreated for 16 h in the presence or absence of CQ for 4 h. GFP-LC3 dots were measured. Data are means ± SD (*** = p &lt; 0.001 versus untreated; ###=p&lt;0.001 versus CQ)."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "(I,J) U2OS cells stably expressing LC3 fused with tandem fluorescent GFP-RFP proteins (GFP-RFP-LC3) were treated with the indicated raising doses of 3,4-DC or 50 µM chloroquine (CQ) for 16 h. After fixation, GFP-LC3 and RFP-LC3 dots were measured by automated image acquisition and analysis. GFP and RFP double positive LC3 dots indicating autophagosomes (GFP+), and GFP negative but RFP positive LC3 dots indicating autolysosomes (GFP-) were counted in (J). Data are means ± SD (* = p &lt; 0.05;** = p &lt; 0.01;*** = p &lt; 0.001). Representative images are shown in I. Scale bar equals 10 µm."
NCBI gene IDs:
"LC3: 81631"
Figure legend: "(C, D) H4-GFP-LC3 cells were treated with 30 µM 3,4-DC in the presence or absence of CHX or AMD or with CQ for 16 h as controls, as indicated. GFP-LC3 dots were quantified in (D). Data are means ± SD (* = p &lt; 0.05, ** = p &lt; 0.01 versus untreated control; # = p &lt; 0.05 versus untreated with CQ)."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: ",G) U2OS GFP-LC3 cells were enucleated to obtain cytoplasts, which were further treated with 3,4-DC and torin1. GFP-LC3 dots in cytoplasts were counted as shown in (G). Data are means ± SD (** = p &lt; 0.01). Representative images with cytoplast marked by a white square are shown in (F). Scale bar equals 10 µm."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "U2OS cells stably expressing GFP-TFEB fusion protein were treated with indicated chalcones at 30 µM for 6 h. GFP intensities in nuclei and cytoplasm were measured and the ratio of GFP intensities in nuclei and cytoplasm were calculated to indicate TFEB translocation to nuclei (A). Data are means ± SD (* = p &lt; 0.05;** = p &lt; 0.01;*** = p &lt; 0.001)."
NCBI gene IDs:
"GFP: 
TFEB: 7942"
Figure legend: "U2OS cells stably expressing GFP-TFEB fusion protein were treated with indicated chalcones at 30 µM for 6 h. Representative images are shown in (B). Scale bar equals 10 µm."
NCBI gene IDs:
"GFP: 
TFEB: 7942"
Figure legend: "MEF wild type and TSC2 knockout (TSC2 KO) cells were treated with 3,4-DC for 6 h. The cells were fixed and immunofluorescence was performed to detect endogenous TFEB. The ratio of TFEB intensities between nuclei and cytoplasm was calculated in (H). Data are means ± SD (*** = p &lt; 0.001 versus Ctr; ###=p&lt;0.001 versus WT/3,4-DC). Representative images are shown in (G). Scale bar equals 10 µm."
NCBI gene IDs:
"TSC2: 22084"
Figure legend: "MEF wild type and TSC2 knockout (TSC2 KO) cells were treated with 3,4-DC for 6 h. (I) After the treatment, nuclei were isolated and western blot was performed to detect nuclear and cytosol TFEB protein levels."
NCBI gene IDs:
"TSC2: 22084"
Figure legend: "(J-L) MEF wild type and TSC2 KO cells were collected and lysed for western blot after the treatment with 3,4-DC. Antibodies against LC3, p62, TSC2, or GAPDH were administrated to detect protein levels, and phosphorylation of mTOR substrate p70 S6K at Threonine 389 (T389) was measured with the phosphorylation specific antibody (P-p70(T389)) and anti-p70 antibody. The bands intensities of LC3-II, p62, and GAPDH were measured with Image J, and the ratios of LC3-II/GAPDH and p62/GAPDH were calculated in (K) and (L). Data are means ± SEM of at least three independent experiments (** = p &lt; 0.01, *** = p &lt; 0.001 vs Ctr; #=p&lt;0.05, ##=p&lt;0.01 vs WT/3,4-DC)"
NCBI gene IDs:
"TSC2: 22084"
Figure legend: "U2OS-GFP-LC3 cells transfected with 3 individual siRNAs specifically targeting TFEB (siTFEB-#1, siTFEB-#2, siTFEB-#3) or scrambled siRNA (siCtr) were treated with 3,4-DC as indicated for 16 h. TFEB knockdown efficiency by TFEB siRNAs and TFEB deficiency by knockout were checked by SDS-PAGE and immunoblot"
NCBI gene IDs:
"GFP: 
LC3: 81631
TFEB: 7942"
Figure legend: "U2OS-GFP-LC3 cells transfected with 3 individual siRNAs specifically targeting TFEB (siTFEB-#1, siTFEB-#2, siTFEB-#3) or scrambled siRNA (siCtr) were treated with 3,4-DC as indicated for 16 h. GFP-LC3 dots were quantified as indicator of autophagy Data are means ± SD (* = p &lt; 0.05, ** = p &lt; 0.01 versus siCtr or WT cells treated with 3,4-DC). Representative images are shown Scale bar equals 10 µm."
NCBI gene IDs:
"GFP: 
LC3: 81631
TFEB: 7942"
Figure legend: "U2OS wild type (wt) and TFEB knockout (TFEB KO) GFP-LC3 cells were treated with 3,4-DC as indicated for 16 h. TFEB knockdown efficiency by TFEB siRNAs and TFEB deficiency by knockout were checked by SDS-PAGE and immunoblot"
NCBI gene IDs:
"GFP: 
LC3: 81631
TFEB: 7942"
Figure legend: "U2OS wild type (wt) and TFEB knockout (TFEB KO) GFP-LC3 cells were treated with 3,4-DC as indicated for 16 h. GFP-LC3 dots were quantified as indicator of autophagy ata are means ± SD (* = p &lt; 0.05, ** = p &lt; 0.01 versus siCtr or WT cells treated with 3,4-DC). Representative images are shown cale bar equals 10 µm."
NCBI gene IDs:
"GFP: 
LC3: 81631
TFEB: 7942"
Figure legend: "U2OS wild type (WT), TFEB or TFE3 knockout, or double knockout (TF DKO) cells were treated with 30 µM 3,4-DC for 16 h. Following, cells were collected and SDS-PAGE and immunoblots were performed as described before. TFEB, TFE3, LC3, p62, and GAPDH protein levels were measured with specific antibodies (G)."
NCBI gene IDs:
"TFE3: 7030
TFEB: 7942"
Figure legend: "U2OS wild type (WT), TFEB or TFE3 knockout, or double knockout (TF DKO) cells were treated with 30 µM 3,4-DC for 16 h. GFP-LC3 dots were quantified as indicator of autophagy (H). Data are means ± SD (* = p &lt; 0.05, ** = p &lt; 0.01, *** = p &lt; 0.001 versus Ctr, respectively)."
NCBI gene IDs:
"TFE3: 7030
TFEB: 7942"
Figure legend: "(I) U2OS WT, knockout for TFEB (TFEB-/-) or TFE3 (TFE3-/-), double knockout (DKO) were treated with 3,4-DC for 24 h and stained with LysoTracker Red for 30 min. Thereafter, the red (positive) dots were measured. Data are means ± SD (* = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001)."
NCBI gene IDs:
"TFE3: 7030
TFEB: 7942"
Figure legend: "(J) Relative mRNA expression levels of key autophagy genes such as Lamp1, Lc3b and p62 were detected in controls and 3,4-DC treated U2OS cells. Data is depicted as a heatmap showing means of at least three independent experiments (*** = p &lt; 0.001 versus WT/Ctr and # = p &lt; 0.05, ## = p &lt; 0.01, as compared to WT treated with 3,4-DC)."
NCBI gene IDs:
"Lamp1: 3916
Lc3b: 81631
p62: 8878"
Figure legend: "GFP-LC3 expressing mice were i.p. injected with 3,4-DC for 24 h. Leupeptin (Leu) was used to test autophagic flux in vivo and GFP-LC3 dots were measured in liver tissue. Data are means ± SEM of at least three mice (*= p &lt; 0.05 versus ctr without Leu; #= p &lt; 0.05 versus ctr with Leu)."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "GFP-LC3 expressing mice were i.p. injected with 3,4-DC for 24 h. Leupeptin (Leu) was used to test autophagic flux in vivo and GFP-LC3 dots were measured in heart (I, J) tissue. Data are means ± SEM of at least three mice (*= p &lt; 0.05 versus ctr without Leu; #= p &lt; 0.05 versus ctr with Leu)."
NCBI gene IDs:
"GFP: 
LC3: 81631"
Figure legend: "(A) Twelve weeks-old wild-type and cardiac specific Atg7 knockout (Atg7cKO) mice were injected with corn oil (vehicle control, Ctr) or 3,4-DC 24 h before surgery and subjected to 3 h prolonged ischemia. Representative images of left ventricular (LV) myocardial sections after alcian blue and triphenyltetrazolium chloride (TTC) staining are depicted. Scale bar equals 1 mm."
NCBI gene IDs:
"Atg7: 74244"
Figure legend: "Growth kinetic of MCA205 fibrosarcomas that were either wild type or Atg5KD (H-I) and were evolving in immunocompetent C57Bl/6 mice or immunodeficient nu/nu mice (G), treated as indicated"
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(J-L) Immunocompetent C57Bl/6 mice were subcutaneously inoculated with TFEB/TFE3 double knockdown MCA205 cells or its scramble control cells (K). When tumors became palpable, mice were treated as indicated Tumor growth curves from mice subjected to 3,4-DC administration alone or in combination with MTX are shown (J,L). Asterisks indicate significant effect of MTX with respect to untreated controls (mean value ±SEM, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001), while hash symbols refer to the comparison of the effects of MTX plus 3,4-DC to MTX alone (#p&lt;0.05, ##p&lt;0.01, ###p&lt;0.001)(n=6-14)."
NCBI gene IDs:
"TFE3: 209446
TFEB: 21425"
Figure legend: "WB analysis of smc5-AID clones in the absence or presence of Auxin using FLAG. Tubulin is used as loading control and wt cells is used as a negative control for the FLAG tag."
NCBI gene IDs:
"smc5: 431608"
Figure legend: "Proliferation curve of wt and smc5-AID cells in the presence or absence of Auxin. The data represents means ± SD of 3 independent experiments."
NCBI gene IDs:
"smc5: 431608"
Figure legend: "Proliferation curves of wt and 2 independent smc5 clones grown at 39.5ºC, with the estimated doubling time on the right. Data represents means ± SD of 4 experiments. Asterisks indicate p value ≤ 0.05, as derived from unpaired t-test type 3, two-sample unequal variance, carried out to check statistical significance between smc5 clones and wt. smc5 clone 1 versus wt, p=0.016, smc5 clone 2 versus wt, p=0.04."
NCBI gene IDs:
"smc5: 431608"
Figure legend: "Bidimensional FACS analysis shows significant accumulation in G2/M and decrease in the S phase in smc5 mutants. The data represents means ± SD of 3 independent experiments for smc5 clones 2 and 3, and 4 independent experiments for wt and smc5 clone 1. Asterisks indicate p value ≤ 0.05, detailed below, using paired t-test. S-phase (smc5 clones 1, 2, 3 versus wt: p= 0.0031; p=0.0002; p=0.004, respectively). G2/M (smc5 clones 1, 2, 3 versus wt: p= 0.029; p=0.0014; p=0.02, respectively)."
NCBI gene IDs:
"smc5: 431608"
Figure legend: "Survival curve of smc5 cells treated with different concentrations of cisplatin (CDDP). Data represents means ± SD of 3 experiments."
NCBI gene IDs:
"smc5: 431608"
Figure legend: "Survival curve of smc5 cells complemented or not with chicken SMC5 cDNA and treated with different concentrations of cisplatin. The experiment is carried out at 39.5ºC. Data represents means ± SD of at least 3 experiments."
NCBI gene IDs:
"smc5: 431608
SMC5: 431608"
Figure legend: "Survival curve of smc5 ku70 cells treated with different concentrations of cisplatin. Data represents means ± SD of the 3 experiments."
NCBI gene IDs:
"smc5: 431608
ku70: 395767"
Figure legend: "Survival curve of smc5 fancc cells treated with different concentrations of cisplatin. Data represents means ± SD of 3 experiments."
NCBI gene IDs:
"fancc: 427468
smc5: 431608"
Figure legend: "Left panel: Growth curve was carried out to test smc5 fancm cell proliferation. Data represents means ± SD of 3 experiments. Right panel: Survival curve of smc5 fancm cells treated with different concentrations of cisplatin. Data represents means ± SD of the 3 experiments."
NCBI gene IDs:
"fancm: 100857997
smc5: 431608"
Figure legend: "Clonogenic assay to test cisplatin sensitivity of smc5 fancm cells in long term assays. Data represents means ± SD of 2 independent experiments with two independent clones analyzed for smc5 fancm."
NCBI gene IDs:
"fancm: 100857997
smc5: 431608"
Figure legend: "Left panel: Growth curve was carried out to test smc5 rad17 (C) cell proliferation. Data represents means ± SD of 3 experiments. Right panel: Survival curve of smc5 rad17 (C) cells treated with different concentrations of cisplatin. Data represents means ± SD of the 3 experiments."
NCBI gene IDs:
"rad17: 404778
smc5: 431608"
Figure legend: "Left panel: Growth curve was carried out to test smc5 ddx11 (D) cell proliferation. Data represents means ± SD of 3 experiments. Right panel: Survival curve of smc5 ddx11 (D) cells treated with different concentrations of cisplatin. Data represents means ± SD of the 3 experiments."
NCBI gene IDs:
"ddx11: 418144
smc5: 431608"
Figure legend: "Left panel: Representative Western blots illustrating the expression of SMC6, SMC5 and FANCD2 in protein extracts from HeLa cells 48 hours after transfection with the indicated siRNAs. Right panel: Relative expression of SMC5, SMC6 and FANCD2. We performed individual experiments for each cell line with technical replicates. In each individual experiment, we first normalized SMC5, SMC6 or FANCD2 expression to that of Vinculin (internal control) and calculated its relative level in each cell line in relation to the target protein/Vinculin ratio of the siLacZ transfected cells, which was set as one in each experiment. Data present the mean+/-S.E.M. and were analyzed by unpaired t-test. SMC5 in siSMC6: n=7 and p=0.0002; SMC5 in siFANCD2: n=7 and p=0.0016; SMC6 in siSMC5: n=8 and p&lt;0.0001; SMC6 in siFANCD2: n=24 and p&lt;0.0001; FANCD2 in siSMC6: n=30 and p&lt;0.0001; FANCD2 in siSMC5: n=10 and p=0.0008."
NCBI gene IDs:
"LacZ: 
FANCD2: 2177
SMC5: 23137
SMC6: 79677"
Figure legend: "Top. Representative images of nuclei (DAPI stained, blue) with RAD51 (green) foci in HeLa cells transfected with the indicated siRNAs and treated with HU or MMC, as indicated in panel A. The scale bar represents 10 μm. Bottom. Histograms presenting the percentage of RAD51 foci-positive cells as evaluated in the different conditions of above. Data represent the means+/-S.E.M. and were analyzed by unpaired t-test. siSMC6 vs siLacZ: n=3, NT, MMC or HU p=n.s.; siFANCD2 vs siLacZ: n=4, NT p=n.s., MMC p=0.0003, HU p=0.0479; siSMC6+siFANCD2 vs siLacZ: n=3, NT p=n.s., MMC p=0.0439, HU p=0.0458."
NCBI gene IDs:
"LacZ: 
FANCD2: 2177
SMC6: 79677"
Figure legend: "Left panel: Representative images of anaphase HeLa cells with micronuclei (white arrows). 48 hours after transfection with indicated siRNAs cells were incubated 18 hours with 2 μg/ml cytochalasyn B to block cytokinesis. The scale bar represents 20 μm. Right panel: Histograms presenting the percentage of binucleate cells with micronuclei. Data represent the mean +/- SEM of 3 independent experiments, t-test, unpaired. siSMC6 vs siLacZ, p=0.0085; siFANCD2 vs siLacZ, p=0.0057; siSMC6+siFANCD2 vs siLacZ, p=0.0019."
NCBI gene IDs:
"LacZ: 
FANCD2: 2177
SMC6: 79677"
Figure legend: "Top: Examples of HeLa mitotic catastrophes and anaphase bridges as observed 48 hours after siRNA transfection. The scale bar represents 20 μm. Bottom: Histograms presenting the percentage of cells with mitotic abnormalities. Data represent the mean +/- S.E.M. of 3 independent experiments. t-test, unpaired. siSMC6 vs siLacZ: Aberrant mitosis p=0.0125, Nuclear bridges p=0.0198, Mitotic catastrophes p=0.0145; siFANCD2 vs siLacZ: Aberrant mitosis p=0.0005, Nuclear bridges p=0.0002, Mitotic catastrophes p=0.0011; siSMC6+siFANCD2 vs siLacZ: Aberrant mitosis p=0.0012, Nuclear bridges p=0.0116, Mitotic catastrophes p=0.0033."
NCBI gene IDs:
"LacZ: 
FANCD2: 2177
SMC6: 79677"
Figure legend: "Immunoprecipitation of FLAG-SMC6 and FLAG-NSMCE2 with FANCI in HEK293T cells overexpressing the SMC6 or NSMCE2, respectively, in the presence or absence of Ethidium bromide (EtBr). The experiment was performed 3 times, and the blot shows a representative experiment. The band under the one indicated as FANCI may represent a degradation product."
NCBI gene IDs:
"NSMCE2: 286053
SMC6: 79677"
Figure legend: "B. BJ ESCs stably co-expressing H2B-GFP (green) and mCherry-H2B (red), hereafter named BJH2B-2FPs. C. ChIP-qPCR analysis of GFP enrichment inside different repetitive elements (ERV, MajSat, IAPs) used as H3K9me3 domain controls, or at the Hoxa11 gene used as a H3K27me3 domain control, or on transcribed genes (Gapdh, Pou5f1, Actb) or actively transcribed gene promoter (Pou5f1-promoter) in BJ WT and BJH2B-GFP ESCs. Gene names are color-coded in red whether they are associated to heterochromatic domains (enriched in H3K9me3); in black to Polycomb domains (enriched in H3K27me3); in blue to active chromatin domains (enriched in H3K4me3). Data are represented as relative enrichments of H2B-GFP versus histone H2B. Data are means ± s.d (n=3 biological replicates for GFP and histone H2B)."
NCBI gene IDs:
"GFP: 
H2B: 
mCherry: 
Actb: 60
Gapdh: 2597
Hoxa11: 3207
Pou5f1: 5460"
Figure legend: "E. Left panel, in vivo FLIM-FRET assay in BJH2B-2FPs stably co-expressing H2B-GFP (green) and mCherry-H2B (red). Mean FRET efficiency is displayed using a continuous pseudo-colour scale from 0 to 40%. Magnification of the FRET map, with discrete high FRET regions indicated by white arrowheads. Scale bars, 5 µm. Right panel, mean distribution of the FRET efficiency related to the pixel fraction from BJH2B-2FPs cells (n = 384 cells)."
NCBI gene IDs:
"H2B: "
Figure legend: "A. Top and bottom panels depict a representative nucleus of BJH2B-2FPs ESC by GFP intensity (nucleosome concentration) and FRET efficiency, respectively. Yellow arrowheads: regions with high nucleosome density but low FRET efficiency; Orange arrowheads: regions with low nucleosome density but high FRET efficiency. Scale bars,10 μm. The right panel correlates FRET efficiency with GFP fluorescence intensity (Pearson correlation coefficient, r = -0.07, n = 106 cells)."
NCBI gene IDs:
"H2B: "
Figure legend: "F. Top and bottom panels depict a representative nucleus of BJH2B-2FPs ESC after 15 min of ATP depletion by GFP intensity (i.e., nucleosome concentration) and FRET efficiency, respectively. Yellow arrows indicate nucleosome-rich foci associated with high levels of FRET. Scale bars,10 µm. The FRET efficiencies (%) from nucleosome-rich foci in untreated and ATP-depleted cells are depicted as Box-and-Whisker plots. The thick line represents median, the boxes correspond to the FRET efficiency (%) values from the 25-75th percentiles of the median, and the whiskers cover the Minimum to Maximum value range. ****, p &lt; 0.0001 (Mann-Whitney test, n = 61 cells). Mean distribution of the FRET efficiency of BJH2B-2FPs from nucleosome-rich foci in untreated (black, n = 162) and ATP-depleted cells (red, n = 61 cells). ****, p = 2.2e-16; K-S test."
NCBI gene IDs:
"H2B: "
Figure legend: "G. Left panel, representative image of in vivo FLIM-FRET measurements from differentiated 3T3 cells co-expressing H2B-GFP and mCherry-H2B. Yellow arrows indicate high FRET efficiencies at H2B-GFP bright chromocenters. Scale bars, 10 µm. Right panel, comparison of the FRET efficiency (%) from ESCs (blue) and 3T3 cells (white) in the GFP-brightest foci (nucleosome-rich foci). FRET efficiency (%) was represented as Box-and-Whisker plots. The thick line represents median, the boxes correspond to the FRET % values from the 25-75th percentiles of the median, and the whiskers cover the Minimum to Maximum value range. ****, p &lt; 0.0001 (Mann-Whitney test, n = 42 cells)."
NCBI gene IDs:
"GFP: 
H2B: 
mCherry: "
Figure legend: "A. Expression of mTagBFP-tagged HP1α in living BJH2B-2FPs and segmentation of HP1α positive foci. Yellow arrowheads: chromocenters enriched in H2B-GFP (left panel), in mTagBFP-HP1α (middle panel), and following segmentation (right panel). B. Box plot of the mean FRET efficiencies of all nucleosome-rich foci (black, n = 68 cells) and foci enriched in HP1α (blue, n = 68 cells). FRET efficiency (%) was represented as Box-and-Whisker plots. The thick line represents median, the boxes correspond to the FRET % values from the 25-75th percentiles of the median, and the whiskers cover the 10-90 percentiles value range. **** p &lt; 0.0001, Mann-Whitney test."
NCBI gene IDs:
"H2B: "
Figure legend: "A. Total cell extracts from BJH2B-2FPs cells incubated during 24h with untargeted siRNA or an siRNA targeting HP1α, analyzed by western blotting with an antiserum against HP1α. Loading control was performed by red ponceau staining."
NCBI gene IDs:
"H2B: 
HP1α: 23468"
Figure legend: "C. Left panel, mean distribution of the FRET efficiency from nucleosome-rich foci of BJH2B-2FPs cells incubated with a control siRNA (black, n = 101 cells) or an siRNA against HP1β (green, n = 136 cells); *, p: 0.02651; K-S test. Right panel, box plot of the mean FRET efficiency from nucleosome-rich foci of living of BJH2B-2FPs cells incubated with a control siRNA (grey, n = 101 cells) or an siRNA against HP1β (green, n = 136 cells). Box-and-Whisker plots represent the FRET efficiency (%). The thick line represents median, the boxes correspond to the FRET % values from the 25-75th percentiles of the median, and the whiskers cover the Minimum to Maximum value range."
NCBI gene IDs:
"H2B: 
HP1β: 10951"
Figure legend: "F. Total cell extracts from untreated, DMSO treated or A-196 treated BJH2B-2FPs during 24h or 48h, analysed by western blotting with antibody against H4K20me3. Arrowhead indicates the H4K20me3-specific bands. The asterisk indicates unspecific bands."
NCBI gene IDs:
"H2B: "
Figure legend: "A. Representative images of immunostainings for Ki-67 in mitotic WT, control (CTRL) and Mki67-/- ESCs. Scale bar, 10 µm."
NCBI gene IDs:
"Mki67: 4288"
Figure legend: "B. Box-and-Whisker plots represent the FRET efficiency (%) from control mitotic ESCs (n = 12 cells) and from three different Mki67-/- ESCs clones named #1 (n = 10 cells), #2 (n = 9 cells) and #3 (n = 8 cells). The thick line represents median, the boxes correspond to the FRET % values from the 25-75th percentiles of the median, and the whiskers cover the 10-90 percentiles value range; ***, p = 0.0007; ****, p &lt; 0.0001, Mann-Whitney test."
NCBI gene IDs:
"Mki67: 4288"