EMBO/SourceData huggingface dataset:
Figure legend: "E Allele distribution of the SlHAK20 locus at position in PIM, CER, and BIG groups. The n indicates the accession number."
NCBI gene IDs:
"SlHAK20: 101256474"
Figure legend: "The expression pattern of SlHAK20 in tomato. The GUS activity was examined in seedling (left) and root (right) of 21-days-old SlHAK20TS-21pro:GUS transgenic tomato (B). Bars, 2 cm."
NCBI gene IDs:
"SlHAK20: 101256474"
Figure legend: "The expression pattern of SlHAK20 in tomato. Cross-sections of hypocotyl (C) of SlHAK20TS-21pro:GUS transgenic plants. The scale bar is 400 μm in (C)"
NCBI gene IDs:
"SlHAK20: 101256474"
Figure legend: "E Subcellular localization of SlHAK20-YFP in tomato. The SlHAK20Hap1-YFP (left) and SlHAK20Hap2-YFP (right) were localized in the plasma membrane. YFP, yellow florescence protein. FM4-64, a lipophilic styryl compound as a red fluorescent marker of plasma membrane. Bar, 20 μm."
NCBI gene IDs:
"SlHAK20: 
SlHAK20: 101256474"
Figure legend: "F SlHAK20 complements the K+ uptake-defective yeast mutant assayed using a dose-response method. The K+ uptake-defective yeast mutant R5421 transformed with SlHAK20Hap1, SlHAK20Hap2, and vector (p416-GPD) were cultured in AP medium containing indicated concentrations of K+ for 24 h. The data points represent three replicates."
NCBI gene IDs:
"SlHAK20: 
SlHAK20: 101256474"
Figure legend: "G Na+ uptake analysis of yeast cells expressing SlHAK20. Shown are the Na+ concentrations in the medium over the time of culture of the ANT3 yeast cells transformed with SlHAK20Hap1, SlHAK20Hap2, or empty vector. The initial NaCl concentration in the medium was 60 μM. The data are shown as the means ± SD (n = 4)."
NCBI gene IDs:
"SlHAK20: 
SlHAK20: 101256474"
Figure legend: "H Na+ uptake kinetic analysis of SlHAK20 in yeast cells. The curves show concentration- dependent Na+ inﬂux in yeast expressing SlHAK20Hap1 or SlHAK20Hap2 in the presence of 0, 5, 10, 20, 30, 40, 50, 60, 100, 150 or 200 μM NaCl in the medium. The data are expressed as mean ± SD (n = 3)."
NCBI gene IDs:
"SlHAK20: 
SlHAK20: 101256474"
Figure legend: "Na+/K+ ratio contents in roots of TS-21 wild type and slhak20 mutants. Data are shown as means ± SD (n = 4)."
NCBI gene IDs:
"slhak20: "
Figure legend: "Na+ contents in roots of TS-21 wild type and slhak20 mutants. Data are shown as means ± SD (n = 4)."
NCBI gene IDs:
"slhak20: "
Figure legend: "K+ contents in roots of TS-21 wild type and slhak20 mutants. Data are shown as means ± SD (n = 4)."
NCBI gene IDs:
"slhak20: "
Figure legend: "Na+ contents in the xylem sap of TS-21 and slhak20 mutants after 50 mM NaCl treatment for the indicated days. Data represent means ± SD (n = 4)."
NCBI gene IDs:
"slhak20: "
Figure legend: "K+ contents in the xylem sap of TS-21 and slhak20 mutants after 50 mM NaCl treatment for the indicated days. Data represent means ± SD (n = 4)."
NCBI gene IDs:
"slhak20: "
Figure legend: "G Shoot height of TS-21, slhak20-1, and slhak20-2. Plant height of 24-day-old TS-21, slhak20-1, and slhak20-2 were measured after growing under normal conditions for 14 days, followed by salt treatment for 14 days, and recovery for 10 days. Values are means ± SD (n = 10 plants of each genotype)."
NCBI gene IDs:
"slhak20: "
Figure legend: "H Salt resistance of TS-21 wild type and slhak20 mutants. TS-21, slhak20-1, and slhak20-2 grown under normal growth conditions for 24 days, followed by a treatment with 200 mM NaCl for 14 days, and then recovered for 10 days. Control plants grown under normal growth conditions for 38 days. Scale bars, 5 cm."
NCBI gene IDs:
"slhak20: 101256474"
Figure legend: " Salt-tolerance assay of TS-670 and slhak20 mutant plants. TS-670, slhak20-3, and slhak20-4 grown under normal growth conditions for 23 days, and then treated with 150 mM NaCl for 5 days. Control plants grown under normal growth conditions for 28 days. "
NCBI gene IDs:
"slhak20: 101256474"
Figure legend: " Plant height and fresh weight of TS-670, slhak20-3, and slhak20-4 under normal and salt stress conditions. Shoot height f 23-day-old TS-670, slhak20-3, and slhak20-4 were quantified after growing under normal conditions for 5 days, or salt treatment with 150 mM NaCl for 5 days. Values are means ± SD (n = 10 plants of each genotype)."
NCBI gene IDs:
"slhak20: 101256474"
Figure legend: " Plant height and fresh weight of TS-670, slhak20-3, and slhak20-4 under normal and salt stress conditions. resh weight f 23-day-old TS-670, slhak20-3, and slhak20-4 were quantified after growing under normal conditions for 5 days, or salt treatment with 150 mM NaCl for 5 days. Values are means ± SD (n = 10 plants of each genotype). "
NCBI gene IDs:
"slhak20: 101256474"
Figure legend: " Phenotype of NIP and oshak4/17 double mutant plants under normal and salt stress conditions. 20-day-old seedlings of NIP and oshak4/17 mutants were treated with 100 mM NaCl for 8 days. The pictures show representative plants ere analyzed among these genotypes. ontrol plants were grown under normal growth conditions for 28 days "
NCBI gene IDs:
"oshak4: 4345789"
Figure legend: " Phenotype of NIP and oshak4/17 double mutant plants under normal and salt stress conditions. 20-day-old seedlings of NIP and oshak4/17 mutants were treated with 100 mM NaCl for 8 days. The pictures show representative fresh weight of shoots were analyzed among these genotypes. Control plants were grown under normal growth conditions for 28 days and the fresh weights of shoots were measured. Values are means ± SD (n = 12 plants of each genotype for fresh weight "
NCBI gene IDs:
"oshak4: 4345789"
Figure legend: " Phenotype of NIP and oshak4/17 double mutant plants under normal and salt stress conditions. 20-day-old seedlings of NIP and oshak4/17 mutants were treated with 100 mM NaCl for 8 days. The pictures show representative survival rate were analyzed among these genotypes. "
NCBI gene IDs:
"oshak4: 4345789"
Figure legend: "A The transcript levels of SlHAK20 in TS-670 and three independent SlHAK20Hap1-YFP transgenic lines grown under normal growth conditions."
NCBI gene IDs:
"YFP: 
SlHAK20: 101256474"
Figure legend: "The Na+ in shoots and roots of TS-670 and three independent SlHAK20Hap1-YFP transgenic lines during salt stress. The 21-day-old TS-670, Hap1OE-1, Hap1OE-2 and Hap1OE-3 plants grown in 0.25× Hoagland which were then treated with 50 mM NaCl for additional 0 and 7 days."
NCBI gene IDs:
"YFP: 
SlHAK20: 101256474"
Figure legend: "The K+ in shoots and roots of TS-670 and three independent SlHAK20Hap1-YFP transgenic lines during salt stress. The 21-day-old TS-670, Hap1OE-1, Hap1OE-2 and Hap1OE-3 plants grown in 0.25× Hoagland which were then treated with 50 mM NaCl for additional 0 and 7 days."
NCBI gene IDs:
"YFP: 
SlHAK20: 101256474"
Figure legend: "The Na+/K+ ratio in shoots and roots of TS-670 and three independent SlHAK20Hap1-YFP transgenic lines during salt stress. The 21-day-old TS-670, Hap1OE-1, Hap1OE-2 and Hap1OE-3 plants grown in 0.25× Hoagland which were then treated with 50 mM NaCl for additional 0 and 7 days."
NCBI gene IDs:
"YFP: 
SlHAK20: 101256474"
Figure legend: "Formalin-fixed paraffin-embedded (FFPE) sections from control or Tgif1KO mice were immunostained with anti-pSmad2 antibody and revealed by immunofluorescence (IF) and DAPI. Representative pictures at 40x are shown (n=30). Scale bars, 100 μM."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "FFPE pancreatic sections from control or Tgif1KO mice were stained with hematoxylin and eosin (H&amp;E) or immunostained with antibodies to CK19 or Amylase and revealed by IHC. Representative pictures at 20x are shown (n=30). Scale bars, 200 μM."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "Kaplan-Meyer survival analysis of control, Tgif1KO, KC and KTC mice. A regular mosaic two-colors line was used to discriminate between control and Tgif1KO mice."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "Pictures of whole pancreas and spleen tissues from control, Tgif1KO, KC or KTC mice (left). Weight of pancreas was measured and presented as dot plot (n=30)."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "FFPE pancreatic sections from control, Tgif1KO, KC or KTC were stained with H&amp;E or subjected to IHC analysis using antibodies to CK19 or α-SMA. Yellow arrows indicate PanINs in KC mice. Representative pictures at 20x are shown (n=30). Scale bars, 200 μM."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "Tumor volume and metastasis in live KCLuc and KTCLuc mice were analyzed by IVIS bioluminescence. Following imaging live animals, pancreas, brain, liver and lung were dissected and analyzed again by IVIS bioluminescence (n= 6, 5 out of 6 mice developed metastasis to liver and lung)."
NCBI gene IDs:
"Luc: "
Figure legend: "Expression of JunB in pancreas from control, KC or KTC mice (same as in D) was analyzed by qRT-PCR (n=6). Data are expressed as mean ± SEM. **p&lt; 0.01; based on a two-tailed Student's test."
NCBI gene IDs:
"JunB: 16477"
Figure legend: "Protein extracts from MIAPaCa-2 cells (left) and control or Tgif1KO mice (right) were immunoprecipitated using normal IgG, anti-TGIF1 or anti-Twist1 antibody and analyzed by immunoblotting using antibodies to Twist1 or TGIF1 (A-C)."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "Protein extracts from control or Tgif1KO mice (right) were immunoprecipitated using normal IgG, anti-TGIF1 or anti-Twist1 antibody and analyzed by immunoblotting using antibodies to Twist1 or TGIF1 To control for loading, total cell lysates were analyzed by immunoblotting using antibodies to Twist1, TGIF1 or β-Actin."
NCBI gene IDs:
"Tgif1: 21815"
Figure legend: "PL45 stably expressing Dox-inducible control or sh-RNA targeting TGIF were treated with vehicle or Dox for 48 hr and analyzed for Twist1 expression by immunoblotting or qRT-PCR (n=6). In left panel, the intensity of the bands was assessed by densitometry, and the result was presented as a ratio of Twist1/TGIF1."
NCBI gene IDs:
"TGIF: 7050"
Figure legend: "PL45 stably expressing Dox-inducible TGIF1 were treated with vehicle or Dox for 48 hr and analyzed for binding of TGIF1 to the Twist1 promoter by ChIP and agarose gel."
NCBI gene IDs:
"TGIF1: 7050
Twist1: 7291"
Figure legend: "Pancreatic chromatin from control or Tgif1KO mice was analyzed for binding of TGIF1 to the Twist1 promoter by ChIP and agarose gel."
NCBI gene IDs:
"Tgif1: 21815
Twist1: 22160"
Figure legend: "PL45 stably expressing both Dox-inducible TGIF1 and Tam-inducible Twist1 (Twist1ER) were treated with Tam and/or Dox for 48 hr and analyzed for Twist1 expression by immunoblotting or qRT-PCR (n=6)."
NCBI gene IDs:
"TGIF1: 7050
Twist1: 7291"
Figure legend: "MIAPaCa-2 cells were transfected with wild-type or mutated (Twist1 binding site) Twist1 luciferase reporters (TGTLuc) together with increasing amounts of Twist1 expression vector in the presence of empty vector (EV) or TGIF1 expression vector. Luciferase activity was measured 48 hr following transfection and normalized on the basis of co-transfected Renilla luciferase (n=6)."
NCBI gene IDs:
"Luc: 
luciferase: 
TGIF1: 7050
Twist1: 7291"
Figure legend: "Expression of Cdh1, Vimentin, and p16Ink4A in pancreas from control, KC or KTC mice (same as in C) was analyzed by qRT-PCR (n=6)."
NCBI gene IDs:
"Cdh1: 12550
p16Ink4A: 12578
Vimentin: 22352"
Figure legend: "Expression of Cdh1, Vimentin and p16Ink4A in pancreas from control, KTC or KTWC mice was analyzed by qRT-PCR (n=6)."
NCBI gene IDs:
"Cdh1: 12550
p16Ink4A: 12578
Vimentin: 22352"
Figure legend: "Specificity of anti-phospho-TGIF1 (pTGIF1) antibody. Cell extracts from Tgif1-/- MEFs cells reconstituted with empty vector (EV), wild-type (TGIF1) or non-phosphorylated form (TGIF1.2TA) of Flag-TGIF1 were immunoblotted with anti-phospho-TGIF1 or β-Actin as a loading control."
NCBI gene IDs:
"Tgif1: 21815
TGIF1: 21815"
Figure legend: "MIAPaCa-2 cells were transfected with TGIF1 or TGIF1.2TD expression vectors, selected with G418 and resistance colonies were pooled (n=6). Expression of Twist1 was examined by qRT-PCR (D)."
NCBI gene IDs:
"TGIF1: 7050
Twist1: 7291"
Figure legend: "MIAPaCa-2 cells were transfected with TGIF1 or TGIF1.2TD expression vectors, selected with G418 and resistance colonies were pooled (n=6). Binding of TGIF1 to the Twist1 promoter was examined by ChIP and agarose gel."
NCBI gene IDs:
"TGIF1: 7050
Twist1: 7291"
Figure legend: "BxPC3 cells were transfected with TGTLuc together with TGIF1 or TGIF1.2TA in the presence or absence of KrasG12D. Luciferase activity was measured 48 hr following transfection and normalized on the basis of co-transfected Renilla luciferase (n=6)."
NCBI gene IDs:
"Luc: 
luciferase: 
Kras: 3845
TGIF1: 7050"
Figure legend: "BxPC3 cells were transfected with CDH1Luc or p16Luc reporter together with TGIF1 or TGIF1.2TA in the presence or absence of KrasG12D and luciferase activity was measured 48 hr following transfection as described in (E) (n=6)."
NCBI gene IDs:
"Luc: 
CDH1: 999
p16: 1029
Kras: 3845
TGIF1: 7050"
Figure legend: "MIAPaCa-2, Suit-2, Capan-2 and Panc-1 cells were transfected with empty vector (EV), wild-type (TGIF1) or phosphorylation mimic mutant (TGIF1.2TD) of TGIF1. Cell extracts were analyzed 48 hr following transfection by immunoblotting using anti-Flag antibody (C)."
NCBI gene IDs:
"TGIF1: 7050"
Figure legend: "MIAPaCa-2, Suit-2, Capan-2 and Panc-1 cells were transfected with empty vector (EV), wild-type (TGIF1) or phosphorylation mimic mutant (TGIF1.2TD) of TGIF1. Cells were selected with neomycin for 2-3 weeks, and counted using an automatic cell counter (D) (n=6)."
NCBI gene IDs:
"TGIF1: 7050"
Figure legend: "(a,b) Dual positive cells are more prevalent in epithelial organs and cells. (a) Proportion of ACE2+TMPRSS2+ cells (y axis) per dataset (dots) from 21 tissues and organs (rows)."
NCBI gene IDs:
"ACE2: 59272
TMPRSS2: 7113"
Figure legend: "(e-i) In situ validation of dual positive cells in the lung, airways, and submucosal gland. (e-g) PLISH and immunostaining in human adult lung alveoli for (e) ACE2 (white), TMPRSS2 (green) and CTSL (red), (f) ACE2 (white), TMPRSS2 (green) and HTII-280 (red), (g) ACE2 (red) and Pro-SFTPC (green). (h) PLISH and immunostaining in human large airway submucosal glands. ACE2 (red), ACTA2 (green) and DAPI (blue). (i) PLISH in human adult large airway. ACE2 (green) and DAPI (blue)."
NCBI gene IDs:
"HTII-280: 
Pro-SFTPC: 
ACE2: 59272
CTSL: 1514
TMPRSS2: 7113"
Figure legend: "(a,c) Single-cell ATAC-seq of lung samples from primary carina (1C) and subpleural parenchyme (RPL) (n=1 patient, k=3 samples, 3,366 cells from 1C, 8,340 cells from RPL). Uniform Manifold Approximation and Projection (UMAP) embedding of scATAC-seq profiles (dots) colored by (a, left) cell types, (a, right) cells with at least 1 fragment (indicating accessibility, open chromatin) mapping to the ACE2 gene locus (defined as −2kb upstream the Transcription Start Site to Transcription End Site), or by sample location"
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "(g) Low expression in pediatric samples. Mean expression level (log CPM, y axis) of ACE2 (blue), TMPRSS2 (orange), and CTSL (green) across age bins (x axis) in AT2 (left) and ciliated (right) cells. Pediatric samples: 0-10 years. Samples from past or current smokers were removed from this plot to avoid smoking confounders. Error bars are omitted due to y-axis limitations. They are typically 10-fold the mean value (Supplementary Table 5). Multiciliated and AT2 cells are shown as these cell types are present in fetal data, and show significant age associations with ACE2 expression."
NCBI gene IDs:
"ACE2: 59272
CTSL: 1514
TMPRSS2: 7113"
Figure legend: "(a) Gradual increase in Ace2 expression by airway epithelial cell type with age. Mean expression (y axis) of Ace2 in different airway epithelial cells (x axis) of mice of three consecutive ages (color legend, upper right). Shown are replicate mice (dots), mean (bar), and error bars (standard error of the mean (SEM))."
NCBI gene IDs:
"Ace2: 70008"
Figure legend: "(b) Increase in proportion of Ace2+Ctsl+ goblet and club cells with age. Percent of Ace2+Ctsl+ cells (x axis) in different airway epithelial cell types (y axis) of mice of three consecutive ages (color legend, upper right). Shown are replicate mice (dots), mean (bar), and error bars (SEM)."
NCBI gene IDs:
"Ace2: 70008
Ctsl: 13039"
Figure legend: "(c-j) Increase in Ace2 expression in secretory cells with smoking. Mice were daily exposed to cigarette smoke or filtered air as control for two months after which cells from whole lung suspensions were analyzed by scRNA-seq (Drop-Seq). (c,d) UMAP of scRNA-seq profiles (dots) colored by experimental group (c) or by Ace2+ cells and indicated double positive cells (d). Alveolar epithelial cells (AT1 and AT2) and airway epithelial secretory and ciliated cells are marked."
NCBI gene IDs:
"Ace2: 70008"
Figure legend: "(c-j) Increase in Ace2 expression in secretory cells with smoking. Mice were daily exposed to cigarette smoke or filtered air as control for two months after which cells from whole lung suspensions were analyzed by scRNA-seq (Drop-Seq). (e) The relative frequency of Ace2+ cells is increased by smoking in airway secretory cells but not AT2 cells. Relative proportion (y axis) of Ace2+ (red) and Ace2- (grey) cells in smoking and control mice of different cell types (x axis)."
NCBI gene IDs:
"Ace2: 70008"
Figure legend: "(f, g) Expression of Ace2 is increased in airway secretory cells, but not in AT2 cells. Distribution of Ace2 expression (y axis) in secretory (f) and AT2 (g) cells from control and smoking mice (x axis)."
NCBI gene IDs:
"Ace2: 70008"
Figure legend: "(h-j) Re-analysis of published bulk mRNA-Seq101 of lungs exposed to different daily doses of cigarette smoke show increased expression of (h) Ace2, (i) Tmprss2, and (j) Ctsl after five months of chronic exposure."
NCBI gene IDs:
"Ace2: 59272
Ctsl: 1514
Tmprss2: 7113"
Figure legend: "(k) Expression in placenta. UMAP plots showing expression of Ace2, Tmprss2 and Ctsl in single and double positive cells from embryonic days 9.5 to 18 (left) and embryonic day 14.5 (right) of mouse placenta development"
NCBI gene IDs:
"Ace2: 70008
Ctsl: 13039
Tmprss2: 50528"
Figure legend: "(a) Multiple proteases are co-expressed with ACE2 in human lung scRNA-seq. Scatter plot of significance (y axis, -log10(adjusted P value)) and effect size (x axis) of co-expression of each protease gene (dot) with ACE within each indicated epithelial cell type (color). Dashed line: significance threshold. TMPRSS2 and PCSKs that significantly co-expressed with ACE2 are marked."
NCBI gene IDs:
"PCSKs: 
ACE2: 59272
TMPRSS2: 7113"
Figure legend: "A HeLa  cells were transfected with control (Ctr) or three different GCN2-targeting siRNA-s and progression  from prophase  to anaphase  was observed by  live-cell imaging. Averages (lines) and SEM (bands) from five (siCtr) or four (each siGCN2) independent experiments are shown; n= 379 for siCtr, 241 for siGCN2 #1, 170 for siGCN2 #2, 239 for siGCN2 #3. ****p&lt;0.0001 for each of the siRNA-s (Kolmogorov-Smirnov test). t=0 denotes entry into prophase  as judged by the first frame showing chromatin  condensation."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "E, Chromosome  congression is affected by the absence of GCN2. E Immunofluorescence  images of HeLa  cells stained  for a kinetochore  marker, CREST  (grey) and DAPI  for DNA (blue). Arrowheads indicate misaligned chromosomes. Scale bars represent 5 µm."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "G­­ Increased number of mitotic  cells with tripolar spindles  after GCN2  knock-down. HeLa  cells were transfected with control (Ctr) or GCN2-targeting siRNA and fixed for immunofluorescence. The number of spindle poles was determined by using a pericentrin  antibody. Mean and SEM are shown from five independent experiments. n= 61, 27, 19, 24 and 28 for siCtr; n=49, 29, 22, 26 and 26 for siGCN2; p=0.0125, paired two-tailed t-test."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "A, B There is no significant difference in global translation rates in mitosis  after GCN2  knock-down. A HeLa  cells were pulse labeled with OPP  and fixed with 70% ethanol. OPP  was detected by Click-IT (AF 647) and mitotic  cells were identified by staining  for phospho-histone H3. 2D  flow cytometry plots are shown, where AF647  intensity corresponds to OPP  incorporation. Translation levels normalized to siCtr G1. The graph shows averages and SEM from three independent experiments. Paired two-tailed t-tests."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "A Verification of the mutants. HeLa  cells transduced with the indicated GCN2-siR constructs were transfected with control (Ctr) or GCN2-targeting siRNA. Halofuginone  (HFG) was added to 40 nM for 4 hours, and GCN2 autophosphorylation, ATF4  induction and eIF2α phosphorylation  were assessed by immunoblotting. γ-tubulin is shown as loading control. The line in the GCN2  blot indicates that a longer exposure of the same blot  is shown on the left than on the right."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "B GCN2 coimmunoprecipitates  with PP1α  and γ. HeLa  cells stably transfected with GFP-PP1α  or GFP-PP1γ  were transduced to express either GCN2-siR (wild-type) or GCN2-siR-AAXA. Cells were  arrested in prometaphase with Eg5i, GFP-PP1α  and GFP-PP1γ  were immunoprecipitated  using GFP-trap and the immunoprecipitates  were immunoblotted  for GCN2. The vertical line in the PP1α  and PP1γ blots  indicates that different exposures of the same blot  are shown for the inputs and the IP-s."
NCBI gene IDs:
"GFP: 
GCN2: 440275
PP1α: 5499
PP1γ: 5501"
Figure legend: "D GCN2   can phosphorylate PP1α  and γ in vitro. HeLa  cells carrying GFP-PP1α  or GFP-PP1γ  were arrested in prometaphase  with an Eg5  inhibitor (Lane 3), then GFP-tagged PP1α  or PP1γ  were immunoprecipitated  using GFP-trap (input, Lane 2). The immunoprecipitates  were incubated in kinase  buffer and ATP  with constitutively active, recombinant GCN2. The samples were run on phos-tag gels and immunoblotted  with PP1γ  or PP1α  antibody."
NCBI gene IDs:
"GFP: 
PP1α: 5499
PP1γ: 5501"
Figure legend: "F PP1α  and PP1γ  bandshifts are diminished in cells depleted for GCN2  and arrested in mitosis. HeLa  cells were transfected with control or GCN2-targeting siRNA and arrested in prometaphase  using a PLK1  inhibitor. Representative quantifications of the ratios of the phospho-PP1/PP1 signal (average and SEM) from four independent experiments are shown. *p=0.0217 (PP1α) and **p=0.0069 (PP1γ) (paired two-tailed t-tests)."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "HeLa  cells were transfected with control or GCN2-targeting siRNA and fixed for immunofluorescence. Representative image of mitotic  cells stained  for A ChTOG  (magenta), tubulin  (green) and DNA  (blue), Scale bars represent 5 µm. Percentage of cells displaying the aberrant localizations  Averages and SEM from three independent experiments are shown. For chTOG  n= 29, 37, 44 for siCtr; n= 14, 41, 28 for siGCN2; **p=0.0063 (paired two-tailed t-tests)."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "HeLa  cells were transfected with control or GCN2-targeting siRNA and fixed for immunofluorescence. Representative image of mitotic  cells stained  for B CENPE  (magenta), P-Aurora B  (yellow), tubulin  (green) and DNA (blue) are shown. Scale bars represent 5 µm. D Percentage of cells displaying the aberrant localizations  Averages and SEM from three independent experiments are shown. For CENPE  n= 15, 35, 43 for siCtr; n= 11, 21, 20 for siGCN2; **p=0.001, for P-Aurora B n=29, 37, 44 for siCtr; n= 14, 41, 28 for siGCN2; **p=0.0053 (paired two-tailed t-tests)."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "HeLa  cells were transfected with control or GCN2-targeting siRNA, synchronized in the cell cycle by thymidine  block-and-release and samples were collected for  flow cytometry  at the indicated time points. A Cell-cycle progression in the cells synchronized by thymidine  block-and-release, three independent experiments, averages and SEM are shown."
NCBI gene IDs:
"GCN2: 440275"
Figure legend: "HeLa  cells were transfected with control or GCN2-targeting siRNA, synchronized in the cell cycle by thymidine  block-and-release and samples were collected for immunoblotting  at the indicated time points. B Representative immunoblots  of TACC3-S558 phosphorylation  and  Aurora A-T288 phosphorylation"
NCBI gene IDs:
"GCN2: 440275"