EMBO/SourceData huggingface dataset:
Figure legend: "(C) Graph relative to Notch3 expression in MuSCs isolated from 1.5 month old mdx mice treated either with vehicle (CTR) or TSA (TSA), and in EVs derived from FAPs (EVs-FAPs) isolated from 1.5 month old mdx mice treated either with vehicle (CTR) or TSA (TSA) (n=3). Star (*) indicates statistical analysis by Tukey test relative to mdx vehicle treated mdx mice (CTR); *p&lt;0,05;. hash (#) means significance compared to EVs-FAPs CTR, ##p&lt;0,01. § indicates significance by Anova test; §§ p&gt;0,01."
NCBI gene IDs:
"Notch3: 18131"
Figure legend: "(D) Graph relative to Notch1 expression in MuSCs isolated from 1.5 month old mdx mice treated either with vehicle (CTR) or TSA (TSA), and in EVs derived from FAPs (EVs-FAPs) isolated from 1.5 month old mdx mice treated either with vehicle (CTR) or TSA (TSA) n=3. Star (*) indicates statistical analysis by Tukey test relative to mdx vehicle treated mdx mice (CTR); *p&lt;0,05;. hash (#) means significance compared to EVs-FAPs CTR; *p &lt; 0.05. § indicates significance by Anova test; §§ p&gt;0,01."
NCBI gene IDs:
"Notch1: 18128"
Figure legend: "(F) From left to right: representative images of myofibers cultured for 24 hours alone (-) or with conditioned media of FAPs that were previously exposed or not to TSA and GW4869 (MEDIA-FAPs) or extracellular vesicles (EVs-FAPs) collected from FAPs exposed or not to TSA in vivo (CTR and TSA) and transfected with antagomiR-206 (TSA A-206). Myofibers have been stained for EdU(Cyan), immunofluorescence with Pax7 (green), MyoD (red). Nuclei were counterstained with DAPI (blue). (n=3). Scale bar = 25 μm. (G) Graph showing the average of nuclei positive for Pax7 (green), MyoD (red) in the conditions indicated in (F). Star (*) indicates statistical analysis by Tukey test relative to myofibers cultured alone (-), (n=3, biological replicates). *p&lt;0,05, **p&lt; 0.01; ***p&lt;0,001; ***p&lt;0,0001; hash (#) means significance compared to MEDIA FAPs CTR, #p&lt;0,05; (†) means significance to EV-FAPs CTR, † p&lt;0,05; while ¶ means significance compare to EVs-FAPs TSA, ¶¶¶¶ p&lt;0,0001. § means significance by Anova test; §§ p &lt; 0.01."
NCBI gene IDs:
"miR-206: 387202"
Figure legend: "The expression of Il1b was measured in whole brain using quantitative PCR 1 hour, 2 hours and 6 hours after I.P. challenge (n=6 mice per group). Data are expressed as mean ± STDEV and were tested by two-way ANOVA with Bonferroni's post-test; *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001. Symbols above bars are versus PBS at the relevant time-point."
NCBI gene IDs:
"Il1b: 16176"
Figure legend: "WT (E-G) and Nlrp3-/- (H-J) mixed glia were primed with MPSIIIA GAG or LPS. ATP (5 mM), cholesterol crystals (1 mg/ml) or amyloid-β fibrils (Aβ1-40 4.3 μM and Aβ1-42 5 μM) were added 4 hours post-priming stimulus, and the secretion of IL-1β into the media measured via ELISA after a further 20 hours (n=3 independent experiments each with 3 inter-experimental replicates). Data are expressed as mean ± STDEV and were tested by one-way ANOVA with Tukey's post-test; *P&lt;0.05, ***P&lt;0.001"
NCBI gene IDs:
"Nlrp3: 216799"
Figure legend: "The biological activity of LV-mediated IL-1Ra was assessed in vitro. RAW264.7 macrophages were transduced with LV.IL1RN or LV.GFP vectors at a MOI of 10. After 72 hours cells were stimulated with recombinant hIL-1β and the expression of TNF-α assessed after a further 48 hours. (n=3 independent experiments each with 3 inter-experimental replicates). Data are expressed as mean ± STDEV and were tested by two-way ANOVA with Bonferroni's post-test; *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001. Symbols above bars are versus NTC within a treatment group."
NCBI gene IDs:
"GFP: 
IL-1Ra: 3557
IL1RN: 3557"
Figure legend: "Human IL-1Ra expression was quantified in the plasma of mice at 6 months of age (4 months post-transplant) (n=10 mice per group). Data are expressed as mean ± STDEV and were tested by one-way ANOVA with Tukey's post-test; WT vs. LV.IL1RN P&lt;0.0001; MPSIIIA vs. LV.IL1RN P&lt;0.0001. Symbols above bars are versus WT."
NCBI gene IDs:
"IL1RN: 3557"
Figure legend: "Quantification of human IL-1Ra levels in the brain of transplanted MPSIIIA mice (n=10 mice per group). Data are expressed as mean ± STDEV and were tested by one-way ANOVA with Tukey's post-test; WT vs. LV.IL1RN P&lt;0.0001; MPSIIIA vs. LV.IL1RN P&lt;0.0001. Symbols above bars are versus WT."
NCBI gene IDs:
"IL1RN: 3557"
Figure legend: "Representative sections from 6-month old MPSIIIA and LV.IL1RN transplanted MPSIIIA mice. Sections show the CA3 region of the hippocampus. Cells expressing high levels of human IL-1Ra are indicated with black arrows. 40x, scale bar, 50 μm (n=4 mice per group)."
NCBI gene IDs:
"IL1RN: 3557"
Figure legend: "(B) Immunoblot  of WT, cGAS  KO, and MAVS  KO THP1 cells Data information: Panel (B) is representative of two independent biological experiments."
NCBI gene IDs:
"cGAS: 115004
MAVS: 57506"
Figure legend: "(D)  RT-qPCR analysis of VZV ORF63  (immediate early (IE) gene), ORF54  (early (E) gene), and ORF40  (late (L) gene) transcripts in cells infected as in (C). Graphs show expression relative to GAPDH. Data information: Panel (D) show pooled data from three repeats, where each data point represents an independent biological experiment (n=3 ± SEM). Statistical analysis in panels (D), was paired t-tests hpi: hours post infection"
NCBI gene IDs:
"GAPDH: 
ORF40: 1487708
ORF54: 1487723
ORF63: 1487711"
Figure legend: "(E) RT-qPCR analysis of IFNB1, IFI44, and IFIT1  expression in cells infected as in (C). Graphs show expression relative to GAPDH. Data information: Panel (E) show pooled data from three repeats, where each data point represents an independent biological experiment (n=3 ± SEM). Statistical analysis in panel (E) paired ratio t-tests. hpi: hours post infection"
NCBI gene IDs:
"GAPDH: 2597
IFI44: 10561
IFIT1: 3434
IFNB1: 3456"
Figure legend: "(F) Activity of  Lucia luciferase (secreted under an IRF3-dependent promotor) was determined in supernatants of cells infected as in (C) by  QUANTI-Luc assay. Data information: Panel , (F), show pooled data from four repeats, where each data point represents an independent biological experiment (n=4 ± SEM). Statistical analysis in panels (F), was paired t-tests hpi: hours post infection, WT: wild type, KO: knockout."
NCBI gene IDs:
"Lucia luciferase: 
IRF3: 3661"
Figure legend: "(A) HEK293T  cells were transfected with pRL-TK, p125-F-Luc, expression plasmids for human cGAS  and human STING, as well as expression plasmids for individual ORFs. The next day luciferase activity  was determined. For each ORF a z-value was calculated and ORFs sorted by descending values. (B) The experiment shown in panel (A) was repeated three times and for each ORF in each experiment a rank was determined according to z-value (highest z-value = rank 1). Ranks are displayed as a heatmap. The first row shows results displayed in panel (A). Data information: Panel (A) is representative of three independent biological experiments, where each data point represents a technical replicate (mean ± SD). The three independent experiments are summarised in panel (B), where the data from (A) forms the first row."
NCBI gene IDs:
"F-Luc: 
cGAS: 115004
STING: 340061
TK: 24271467"
Figure legend: "(C) HEK293T  cells were transfected as in (A,B) with expression constructs for VZV ORF9  or empty vector. In parallel, reporter expression was stimulated by co-transfection of the RIG-I-CARD plasmid instead of hcGAS  and hSTING. Data information Panel (C) is representing two independent biological experiments, where each data point represents a technical replicate (mean ± SD)."
NCBI gene IDs:
"cGAS: 115004
RIG-I: 23586
ORF9: 1487674
STING: 340061"
Figure legend: "(D) Western blot  analysis of PMA-differentiated THP1 monocytes  stably transduced with either V5-tagged VZV ORF9  or GFP  using the indicated antibodies. Data information: Panels (D) are representing two independent biological experiments. In (E) each data point represents a technical replicate."
NCBI gene IDs:
"GFP: 
V5: 
ORF9: 1487674"
Figure legend: "(E) THP1 monocytes  stably transduced with either VZV ORF9  or GFP were PMA-differentiated and transfected with indicated doses of dsDNA. Expression of IFNB1  and IFNL1  was assessed by RT-qPCR. Graphs show expression relative to GAPDH. Data information: Panels (E) are representing two independent biological experiments. In (E) each data point represents a technical replicate."
NCBI gene IDs:
"GFP: 
GAPDH: 2597
IFNB1: 3456
IFNL1: 282618
ORF9: 1487674"
Figure legend: "(F) THP1 monocytes  stably transduced with either VZV ORF9  or GFP  were treated with the STING  agonist diABZi  (0.005 μM). Expression of IFNB1, IFIT1  and IFI44  was assessed by RT-qPCR. Graphs show expression relative to GAPDH. Data information: Panel (F) shows pooled data from three and two repeats, respectively, where each data point represents an independent biological experiment (horizontal bars show means). Statistical analysis in (F) were paired t-tests."
NCBI gene IDs:
"GFP: 
GAPDH: 2597
IFI44: 10561
IFIT1: 3434
IFNB1: 3456
ORF9: 1487674"
Figure legend: "(I) PMA-differentiated THP1  cells were co-cultured with VZV-ORF9ecDHFR-infected MeWo  cells for 1h in the presence of TMP, washed, and incubated for another 24h in the presence of TMP. Cells were then washed extensively and fresh medium was added that was supplemented or not with TMP. Expression levels of IFNB1, IFIT1, and IFI44  were measured by  RT-qPCR after a further 24h incubation. Graphs show expression relative to GAPDH. Mean fold changes are indicated. Data information: Panel (I) shows pooled data from three repeats, where each data point represents an independent biological experiment. Statistical analysis in (I) were paired t-tests."
NCBI gene IDs:
"DHFR: 944790
GAPDH: 2597
IFI44: 10561
IFIT1: 3434
IFNB1: 3456
ORF9: 1487674"
Figure legend: "(B) THP1 monocytes  stably transduced with either VZV FLAG-ORF9  or FLAG-GFP  were PMA-differentiated overnight. The next day, cells were lysed and ectopically expressed proteins were immunoprecipitated  using α-FLAG  antibody. Input, unbound and IP  fractions were subjected to immunoblotting  using the indicated antibodies. Data information: Panels (B), are representative of three independent experiments."
NCBI gene IDs:
"FLAG: 
GFP: 
ORF9: 1487674"
Figure legend: "(C) HaCaT  cells and PMA-differentiated THP1  cells were infected with WT VZV  or VZVORF9-V5 through co-culture with infected MeWo  cells for 48 hours. Cells were lysed and ORF9  was immunoprecipitated  using α-V5 antibody. Input and IP  fractions were subjected to immunoblotting  using the indicated antibodies. Data information: Panel (C) is representative of two (HaCaT) and three (THP1) independent experiments."
NCBI gene IDs:
"V5: 
ORF9: 1487674"
Figure legend: "(D) HEK293T  cells were seeded onto glass coverslips and were transfected with human cGAS-V5, FLAG-ORF9, or both together. The next day, cells were fixed, permeabilised and stained  using α-V5-FITC, rabbit-α-FLAG, and goat-α-rabbit-AF647  antibodies, and DAPI. Mounted coverslips were imaged using  confocal microscopy. Scale bars: 15 µm. Data information: Panels (D), are representative of three independent experiments."
NCBI gene IDs:
"FLAG: 
V5: 
cGAS: 115004
ORF9: 1487674"
Figure legend: "(B) HEK293T  cells were transfected with expression plasmids for human cGAS   and ORF9  truncation mutants. The next day cells were lysed and ORF9  proteins were immunoprecipitated  using α-FLAG  antibody. IP  fractions were subjected to immunoblotting  using the indicated antibodies. Data information: Results shown in panels (B) are representative of two independent experiments."
NCBI gene IDs:
"ORF9: 1487674"
Figure legend: "(E) HEK293T  cells were transfected with expression plasmids for FLAG-GFP  or FLAG-ORF9. The next day cells were lysed, and the lysate was spiked with biotinylated VACV70mer dsDNA. DNA  was precipitated using streptavidin  beads and fractions were subjected to immunoblotting  using the indicated antibodies. Data information: Results shown are representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
GFP: 
ORF9: 1487674"
Figure legend: "(C) HEK293T  cells were transfected with expression plasmids for FLAG-GFP  or FLAG-ORF9  as indicated. The next day cells were lysed, and the lysate was spiked with biotinylated VACV70mer dsDNA. DNA  was precipitated using streptavidin  beads and proteins were analysed by immunoblotting  using the indicated antibodies. Data information: Data shown in panels (C) are representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
GFP: 
ORF9: 1487674"
Figure legend: "(D) HEK293T  cells were transfected with expression plasmids for human cGAS  and FLAG-GFP  or FLAG-ORF9. The next day cells were lysed, and proteins were immunoprecipitated  using α-FLAG  antibody and analysed by immunoblotting  using the indicated antibodies. Data information: Data shown in panels (D) are representative of two independent experiments."
NCBI gene IDs:
"FLAG: 
GFP: 
ORF9: 1487674"
Figure legend: "(E) THP1  cells stably transduced with GFP, ORF9, or ORF9-DM were transfected with the indicated amounts of E.coli dsDNA. The next day, IFNB1  expression was assessed by RT-qPCR. Data information: Panel (E) shows pooled data from two repeats, where each data point represents an independent biological experiment (n=2 +/- range)."
NCBI gene IDs:
"GFP: 
IFNB1: 3456
ORF9: 1487674"
Figure legend: "(D) ATG5 and ATG7 mRNA levels in indicated tissues (n=4)"
NCBI gene IDs:
"ATG5: 11793
ATG7: 74244"
Figure legend: "(J) Adβ3 mRNA in eWAT from 10‐mo‐old RD‐fed Con and KO mice (n=4), and in (K) inguinal (ingWAT) from 4‐mo‐oldcold‐challenged RD‐fed Con and KO mice (n=3)."
NCBI gene IDs:
"Adβ3: 11556"
Figure legend: "(A,B) Tmem26 and tbx1 mRNA levels in epididymal eWAT, and (C,D) ingWAT from Atg7Flox/Floxmice (4 months (mo)) injected in BAT with empty (Con AdV) or Cre‐expressing (Cre AdV) adenoviruses and cold‐exposed or not (basal) for 75 min (n=4-5)."
NCBI gene IDs:
"Cre: 
Atg7: 74244
tbx1: 21380
Tmem26: 327766"
Figure legend: "(E,F) hematoxylin and eosin (HE)‐stained ingWAT section from Atg7Flox/Flox mice (4 months (mo)) injected in BAT with empty (Con AdV) or Cre‐expressing (Cre AdV) adenoviruses and cold‐exposed or not (basal) for 75 min (n=4-5)."
NCBI gene IDs:
"Cre: 
Atg7: 74244"
Figure legend: "(H) Expression of MuRF‐1 and Atrogin‐1 in GA and TA from 10‐mo‐old Con and KO mice (n=4)"
NCBI gene IDs:
"Atrogin‐1: 67731
MuRF‐1: 433766"
Figure legend: "(K) mRNA for IRS1, (L) IRS2 in epididymal white adipose tissue (eWAT) and GA from 10‐mo‐old Con and KO mice (n=6)."
NCBI gene IDs:
"IRS1: 16367
IRS2: 16368"
Figure legend: "Fold enrichment of γH2AX at telomere repeats by real-time PCR. Graph represents fold enrichment of γH2AX at telomeric repeats between IgG control, 3 and 30 month old whole mouse hearts. Data are mean ± SEM of n=3 mice per age group."
NCBI gene IDs:
"γH2AX: 15270"
Figure legend: "% of telomere FISH signal loss in 30 months old wild-type mice and late generation Terc-/- mice (6 months old) in comparison to 4 months old wild-type mice. Data are from n=3 mice. >100 CMs were analysed per mouse."
NCBI gene IDs:
"Terc: 21748"
Figure legend: "Representative images of rat neonatal CMs 4 days following transfection with a FLAG-tagged TRF1-FokI-D450A (top row) or TRF1-FokI (middle and bottom row) fusion protein (cell treatments the same for all subsequent panels in Figure) (red - telo-FISH; green - γH2A.X). Images are z-projections of 0.1µm stacks taken with 100X objective. White arrows indicate co-localisation between telomeres and γH2A.X, with co-localising foci amplified in the right panels (taken from single z-planes where co-localisation was found). Scale bar represents 3.5μm. Scale bar in magnified images showing individual co-localisation 500nm."
NCBI gene IDs:
"FLAG: 
FokI: 
TRF1: "
Figure legend: "% of γH2A.X foci co-localising with telomeres (B) in FLAG-tagged TRF1-FokI-D450A- and TRF1-FokI-expressing CMs. Data are mean ± SEM of n=4 independent experiments. &gt;50 cells were analysed per condition. Statistical analysis was performed by two-tailed t-test ***P&lt;0.001."
NCBI gene IDs:
"FLAG: 
FokI: 
TRF1: "
Figure legend: "mean number of Telomere-associated Foci (TAF) (C) in FLAG-tagged TRF1-FokI-D450A- and TRF1-FokI-expressing CMs. Data are mean ± SEM of n=4 independent experiments. &gt;50 cells were analysed per condition. Statistical analysis was performed by two-tailed t-test ***P&lt;0.001."
NCBI gene IDs:
"FLAG: 
FokI: 
TRF1: "
Figure legend: "Mean % of FLAG-labelled CMs positive for SA-β-Gal activity. Data are mean ± SEM of n=3 independent experiments. &gt;100 cells were quantified per condition. Statistical analysis performed using two tailed t test; **P&lt;0.01."
NCBI gene IDs:
"FLAG: "
Figure legend: "Expression of p21 mRNA (as a function of β-actin and Gapdh) by real-time PCR in TRF1-FokI-D450A and TRF1-FokI expressing CMs. Data are mean ± SEM of n=6 independent experiments. Statistical analysis performed using two tailed t test; ***P&lt;0.001."
NCBI gene IDs:
"FokI: 
Gapdh: 
TRF1: 
β-actin: 
p21: 114851"
Figure legend: "Mean± SEM cell surface area (μm2) of FLAG-labelled CMs expressing TRF1-FokI-D450A and TRF1-FokI. Statistical analysis performed using two tailed t test; ***P&lt;0.001. Scale bar represents 50μm."
NCBI gene IDs:
"FLAG: 
FokI: 
TRF1: "
Figure legend: "(Above) RNA-sequencing of purified CMs from 4 mice per age group reveal age-dependent increased expression of 3 secreted proteins Edn3, Tgfb2, and Gdf15; the colour intensity represents column Z-score, where red indicates highly and blue lowly expressed. (Below) mRNA expression of Edn3, Tgfb2, and Gdf15 was independently validated by RT-PCR in young and old isolated adult CMs. Data are mean ±S.E.M of n=8 mice per group."
NCBI gene IDs:
"Edn3: 13616
Gdf15: 23886
Tgfb2: 21808"
Figure legend: "Real-time PCR gene expression analysis of MAO-A, MnSOD and catalase in isolated mouse CMs from young (3 months) and old (20 months) mice."
NCBI gene IDs:
"catalase: 12359
MAO-A: 17161
MnSOD: 20656"
Figure legend: "Mean % of TAF-positive nuclei (left graphs) or mean % of TAF (right graphs) in wild-type (control) compared to MAO-A transgenic mice with or without drinking water supplemented with 1.5g/kg/day NAC from the age of 4 to 24 weeks Data are mean ±S.E.M of n=3-4 per group. &gt;100 CMs were quantified per age group."
NCBI gene IDs:
"MAO-A: 29253"
Figure legend: "Mean % of TAF-positive nuclei (left graphs) or mean % of TAF (right graphs) in MnSOD+/+ vs MnSOD-/+, Data are mean ±S.E.M of n=3-4 per group. &gt;100 CMs were quantified per age group."
NCBI gene IDs:
"MnSOD: 20656"
Figure legend: "Mean % of TAF-positive nuclei (left graphs) or mean % of TAF (right graphs) in Catalase+/+ vs. Catalase-/-, Data are mean ±S.E.M of n=3-4 per group. &gt;100 CMs were quantified per age group."
NCBI gene IDs:
"Catalase: 12359"
Figure legend: "Mean % of TAF-positive nuclei (left graphs) or mean % of TAF (right graphs) in WT vs. POLG double mutant mice. Data are mean ±S.E.M of n=3-4 per group. &gt;100 CMs were quantified per age group."
NCBI gene IDs:
"POLG: 18975"
Figure legend: "Comparison between the % of p16- or eGFP-positive CMs by RNA-in situ hybridization per plane in INK-ATTAC mice (28-29 month old) treated with vehicle or AP20187. Data are mean ± SEM of n=5 per age group. 100 CMs were analysed per mouse."
NCBI gene IDs:
"eGFP: 
p16: 12578"
Figure legend: "BACE1 or APP was immunoprecipitated from mouse brains and blotted with E4-PHA lectin (lower) or anti-BACE1 or APP antibodies (upper). hAPP indicates the APP23 transgenic mouse model for AD."
NCBI gene IDs:
"APP: 351"
Figure legend: "APP metabolites from 3-month-old mouse brain membrane (A) or soluble (B) fractions were immunoblotted. The signal intensity was quantified (n = 4-5). All graphs show means ± SEM (*P &lt; 0.05; Student's t-test for (A) βCTF and (B) sAPPα, and Mann-Whitney U-test for the others. P = 0.386 for APP, P = 0.602 for αCTF, P = 0.045 for βCTF, P = 0.218 for sAPPα, P = 0.022 for sAPPβ)."
NCBI gene IDs:
"APP: 351"
Figure legend: "Amounts of Aβ40 or Aβ42 in the TBS-soluble or Gu-HCl-extractable fraction from (A) 3-month-old or (B) 12-month-old hAPP/Mgat3+/+, hAPP/Mgat3+/−, or hAPP/Mgat3−/− brains (n = 5). P = 0.015 for TBS Aβ40, P = 0.034 for TBS Aβ42, P = 0.001 and 0.012 for Gn-HCl Aβ40, P = 0.024 for Gn-HCl Aβ42 in (A), P = 0.040 for Gn-HCl Aβ40, P = 0.047 for Gn-HCl Aβ42 in (B)."
NCBI gene IDs:
"APP: 351
Mgat3: 17309"
Figure legend: "Amounts of Aβ40 or Aβ42 in the TBS-soluble or Gu-HCl-extractable fraction from 3- to 4-month-old Mgat3+/+ or Mgat3−/− brains (n = 7-8). An outlier value was rejected by the Smirnov-Grubbs' test (P &lt; 0.05). P = 0.045 for Gn-HCl Aβ40."
NCBI gene IDs:
"Mgat3: 17309"
Figure legend: "The Y-maze test was performed using 12-month-old male Mgat3+/+, Mgat3−/−, hAPP/Mgat3+/+, or hAPP/Mgat3−/− mice (n = 8-10). **P = 0.004 for Mgat3+/+ versus hAPP/Mgat3+/+, *P = 0.022 for hAPP/Mgat3+/+ versus hAPP/Mgat3−/−."
NCBI gene IDs:
"APP: 351
Mgat3: 17309"
Figure legend: "Immunostaining of 12-month-old mouse cerebral cortex for BACE1 and APP. A typical image in the vicinity of plaque-forming area is shown for hAPP/Mgat3+/+ brain. The area of co-localization was quantified using random images of cerebral cortex (n = 10). *P = 0.015."
NCBI gene IDs:
"APP: 351
Mgat3: 17309"
Figure legend: "MEFs were transfected with control siRNA or GGA3-siRNA and then immunoblotted for BACE1, GGA3, or GAPDH (loading control) (n = 6)."
NCBI gene IDs:
"GGA3: 260302"
Figure legend: "Membrane fractions from 3-week-old Mgat3+/+, Mgat3−/−, and Bace1−/− mice were immunoblotted for CHL1 (upper), contactin-2 (middle), or syntaxin 6 (lower) (n = 4-5). The graphs show means ± SEM (**P &lt; 0.01; two-way ANOVA with post hoc Tukey-Kramer test, P = 0.006 for Mgat3+/+ versus Bace1−/−, P = 0.008 for Mgat3−/− versus Bace1−/− in CHL1/syn6, P = 0.001 for Mgat3+/+ versus Bace1−/−, P = 0.002 for Mgat3−/− versus Bace1−/− in Contactin-2/syn6)."
NCBI gene IDs:
"Bace1: 23821
Mgat3: 17309"
Figure legend: "B mRNA expression of adipogenesis-related factors of SVF cells isolated from iWAT of male mice. D mRNA expression of PPARγ, C/EBPα and FABP4 expression of 3T3-L1 cells treated with curcumin."
NCBI gene IDs:
"C/EBPα: 12606
FABP4: 11770
PPARγ: 19016"
Figure legend: "K Oil Red O staining of Vector or OE-ALKBH5 transfected 3T3-L1 cells. Differentiation was induced with curcumin up to day 8."
NCBI gene IDs:
"ALKBH5: 268420"
Figure legend: "E Oil Red O staining of control, ALKBH5-depleted and ALKBH5+TRAF4 depleted cells after induced for 8 days."
NCBI gene IDs:
"ALKBH5: 268420
TRAF4: 22032"
Figure legend: "Western blot analysis of YTHDF1, ALKBH5 and TRAF4 in control, ALKBH5-depleted and ALKBH5 + YTHDF1 depleted cells and quantification of protein levels normalized to β-actin expression."
NCBI gene IDs:
"ALKBH5: 268420
YTHDF1: 228994"
Figure legend: "L Oil Red O staining of control, ALKBH5-depleted and ALKBH5+YTHDF1 depleted cells on day 8 of differentiation."
NCBI gene IDs:
"ALKBH5: 268420
YTHDF1: 228994"
Figure legend: "M qPCR analysis of PPARγ, C/EBPα and FABP4 expression in cell β-Actin was used as an internal control."
NCBI gene IDs:
"β-Actin: 
C/EBPα: 12606
FABP4: 11770
PPARγ: 19016"
Figure legend: "C, D Western blot analysis of PPARγ protein levels in control and TRAF4 overexpressing cells in the absence or presence of MG132 or 3MA and quantification of protein levels normalized to β-actin expression."
NCBI gene IDs:
"TRAF4: 22032"
Figure legend: "E Ubiquitination of endogenous PPARγ in control and TRAF4 overexpressing cells were differentiated by treatment with DMI. Cells were treated with MG132 for 6 h."
NCBI gene IDs:
"TRAF4: 22032"