EMBO/SourceData huggingface dataset:
Figure legend: "(C) ROS burst response in Col-0, map4k4-1, bik1 and map4k4-1 bik1 mutants. Values are means ± SEM, n = 16. Results are representative of three independent experiments. Data information: Different letters indicate statistical significant base on one-way ANOVA with Tukey post-test, samples sharing letters are not significantly different, p &lt; 0.05."
NCBI gene IDs:
"map4k4: 831324
bik1: 818549"
Figure legend: "(D) Relative expression level of PR1 in Col-0, map4k4-1, bik1 and map4k4-1 bik1mutants by qRT-PCR analysis. Values are means ± SD, n = 3 (biological replicates). Data information: Different letters indicate statistical significant base on one-way ANOVA with Tukey post-test, samples sharing letters are not significantly different, p &lt; 0.05."
NCBI gene IDs:
"map4k4: 831324
bik1: 818549
PR1: 815949"
Figure legend: "(E) Morphological phenotype of 6-week-old of Col-0, map4k4-1, map4k3 and map4k4-1 map4k3 mutants."
NCBI gene IDs:
"map4k4: 831324
map4k3: 843253"
Figure legend: "(F) Relative expression level of PR1 in Col-0, map4k4-1, map4k3 and map4k4-1 map4k3 mutants by qRT-PCR analysis. Values are means ± SD, n = 3 (biological replicates). Data information: Different letters indicate statistical significant base on one-way ANOVA with Tukey post-test, samples sharing letters are not significantly different, p &lt; 0.05."
NCBI gene IDs:
"map4k4: 831324
PR1: 815949
map4k3: 843253"
Figure legend: "(G) ROS burst response in Col-0, map4k4-1, map4k3 and map4k4-1 map4k3 mutants. Values are means ± SEM, n = 16 (biological replicates). Data information: Different letters indicate statistical significant base on one-way ANOVA with Tukey post-test, samples sharing letters are not significantly different, p &lt; 0.05."
NCBI gene IDs:
"map4k4: 831324
map4k3: 843253"
Figure legend: "C Graph showing gene expression differences (log10-fold changes) of defined genes (X-axis) in Tsc2-deficient relative to wild-type MEFs grown in DMEM 10% FBS. The asterisks indicate significant differences with two-sided t-test (**P &lt; 0.01, ***P &lt; 0.001, and ****P &lt; 0.0001; Aldh1a3 P = 0.006, Aldh2 P = 2x10-5, Aldh3a1 P = 0.004; Aldh3b1 P = 0.006; Aldh3b2, P = 0.17, Maoa P = 7x10-5, and Maob P = 7x10-4; replicates/condition n = 3, assays n = 4). The bars indicate mean ± SD. Dotted horizontal lines indicate 2-fold (top) and 0.5-fold (bottom)."
NCBI gene IDs:
"Aldh1a3: 56847
Aldh2: 11669
Aldh3a1: 11670
Aldh3b1: 67689
Aldh3b2: 621603
Maoa: 17161
Maob: 109731
Tsc2: 22084"
Figure legend: "D Western blot  results (independent experiments n = 4) from Tsc2-deficient and wild-type  MEFs exposed to DMSO or treated with 20 nM everolimus for 16 hours in DMEM 10%  FBS. Loading controls are shown. The inferred  protein expression level is indicated by the ratio between the corresponding signal and loading control, standardized to the basal setting"
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "A No differences of Vdac1/VDAC1 expression between MEF cell lines (replicates/condition n = 3 and independent experiments n = 2). The graph shows fold change expression (mean ± SEM) in Tsc2-deficient relative to wild-type  MEFs."
NCBI gene IDs:
"Tsc2: 22084
Vdac1: 22333"
Figure legend: "B  Flow cytometry  results (independent experiments n = 3) showing a higher percentage of MitoSOX red-positive cells in Tsc2-deficient MEFs. C Flow cytometry results (independent experiments n = 3) showing higher intensity (X-axis, single channel intensity FL3 670 nm) of MitoTracker red-positive cells in  Tsc2-deficient MEFs. "
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "D Higher  basal cell respiration (as measured by oxygen consumption, Y-axis) in Tsc2-deficient MEFs. The asterisks indicate significant difference with two-sided t-test (P = 5x10-4; replicates/condition n = 5, and independent experiments n = 3)."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "F Left panel, western blot results showing overexpression of catalase (CAT) in Tsc2-deficient MEFs, and unaffected by exposure to everolimus (independent experiments n = 2). The CAT expression level is indicated by the ratio of the corresponding signal relative to loading control and basal setting (noted as 1(ref)). Right panel, Cat overexpression (log10-fold change) in  Tsc2-deficient MEFs. The asterisks indicate significant difference with two-sided t-test (P = 1x10-2; replicates/condition n = 3, and independent experiments n = 2). The bars indicate mean ± SD. Dotted horizontal lines indicate 2-fold (top) and 0.5-fold (bottom)."
NCBI gene IDs:
"Cat: 12359
Tsc2: 22084"
Figure legend: "A Overabundance of histamine in Tsc2 wild-type relative to Tsc2-deficient MEFs, both growth in DMEM 10% FBS. The asterisks indicate significant difference with two-sided t-test (P = 0.017); replicates/condition n = 5, and independent experiments n = 2). Average values are indicated with lilac-colored lines."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "D  Western blot results of HRH1, SLC22A3, and loading control from Tsc2-deficient and wild-type MEFs exposed to DMSO or treated with 20 nM everolimus for 16 hours in DMEM 10% or 0.5% FBS  (independent experiments n = 2). The expression levels are indicated by the ratio of the corresponding signal relative to loading control and basal setting"
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "F Evaluation of cell viability inhibition by  loratadine alone (concentrations shown on X-axis, from 0 to 100 μM) or combined with everolimus (fixed to 20 nM) in Tsc2-deficient and wild-type MEF cultures grown in DMEM 10% FBS for 72 hours. The synergistic combination index (CIx &lt; 1) is shown (replicates/condition n = 3, independent experiments n = 3). Each data point represents the mean and SD."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "H Percentages of viability of Tsc2-dificient and wild-type MEFs exposed to DMEM 10% or 0.5%  FBS with or without L-histidine for 72 hours. The asterisks indicate significant differences with one-sided t-test (10%  FBS, P = 0.017; 0.5%  FBS, P = 5x10-4; replicates/condition n = 4-6, independent experiments n = 2). The bars indicate mean ± SD."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "B Inhibition of Tsc2-deficient 105K tumor growth with different monotherapies, as indicated in the inset. Asterisks indicate significant reductions relative to vehicle (two-way ANOVA; clorgyline P = 0.015, loratadine P = 0.018, and rasagiline P = 0.049). Each data point represents the mean and SEM."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "C Further reduction of Tsc2-deficient 105K tumor growth by  rapamycin  combined with clorgyline or loratadine, relative to rapamycin alone. Asterisks indicate significant reductions relative to  rapamycin alone (two-way ANOVA; clorgyline P = 0.035, loratadine P = 0.045). Each data point represents the mean and SEM."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "E Inhibition of Tsc2-deficient 105K tumor growth with Maoa expression depletion using shRNAs. Asterisks indicate significant reductions relative to control pLKO (two-way ANOVA; P = 1x10-3). Each data point represents the mean and SEM."
NCBI gene IDs:
"Maoa: 17161
Tsc2: 22084"
Figure legend: "F Inhibition of Tsc2-deficient 105K tumor growth with Hrh1 expression depletion. Asterisk indicates significant reduction relative to control pLKO (two-way ANOVA; P = 0.035) . Each data point represents the mean and SEM. G Inhibition of Tsc2-deficient 105K tumor growth with administration of α-methyl-DL-histidine. Asterisk indicates significant reduction relative to vehicle (two-way ANOVA; P = 0.028). Each data point represents the mean and SEM. "
NCBI gene IDs:
"Hrh1: 15465
Tsc2: 22084"
Figure legend: "E Representative images of  hematoxylin-eosin-stained Tsc2-deficient 105K tumors treated with vehicle or monotherapies. Tumors treated with rapamycin tend to have a fascicular growth pattern with bundles of spindle cells and foci of fibrosis, whereas most of the tumors of the other treatment groups showed extensive epithelioid morphology. Scale bars are shown."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "F Representative images of hematoxylin-eosin-stained Tsc2-deficient 105K tumors treated with rapamycin alone or rapamycin combinations. With the addition of a second drug to rapamycin, tumors more frequently tended to show glandular differentiation and less atypia. Scale bars are shown."
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "C, AltFUS (arrow) endogenous expression in Human tissues (brain, muscles and kidney - 100 μg), in HEK293 and HeLa cultured cells (100 μg) and using the over-expression of altFUS CDS in HEK293 cells (50 μg) as positive control (representative image from n=3). The asterisk indicates a protein species detected with the anti-altFUS antibody specifically in the brain."
NCBI gene IDs:
"altFUS: "
Figure legend: "I, Endogenous altFUS mitochondrial expression in HEK293 cells following fractionation (representative image from n=3), with Tubulin as a marker of the cytosolic fraction and VDAC as a marker of the mitochondrial fraction. We used siFUS transfected cells as a negative control and altFUS transfected cells as a positive control for altFUS expression (WCL = Whole Cell Lysate, Cyto = Cytosol fraction, Mito = mitochondrial fraction)."
NCBI gene IDs:
"altFUS: 
FUS: 2521"
Figure legend: "J, Representative images of the mitochondrial network (Tomm20 in red) in mock and altFUS-Flag (green) transfected HeLa cells (n=3). The white scale bar corresponds to 10μm. K, Proportion of tubules and globules in the mitochondrial network of mock HeLa cells and HeLa cells transfected with altFUS-Flag Quantification was done over a minimum of 100 cells across a technical duplicate per independent experiments (n=3, i.e. a minimum of 300 cells per biological conditions, p-value &lt; 0.001, Mann-Whitney U test). The boxes extend to the 25th and 75th percentiles, with the median marked. The whiskers correspond to the 5th and 95th percentiles."
NCBI gene IDs:
"altFUS: 
Flag: "
Figure legend: "E, Scatter plot of the proteins identified by AP-MS indicating their enrichment (Fold-change over control) and their SAINT probability score. Proteins above the 0.8 threshold (grey line) are indicated in black, others in grey. AltFUS is indicated in red (bait) and preys known to regulate the autophagy or the cellular stress response are indicated in green."
NCBI gene IDs:
"AltFUS: "
Figure legend: "B, LC3-II accumulation after bafilomycin treatment from mock, altFUS, FUS, FUS(Ø), FUS-R495x, FUS(Ø)-R495x transfected cells and FUS(Ø)-R495x and altFUS co-transfected cells across 3 independent experiments (n=3, mean ± SD). The quantification corresponds to the treated/untreated ratio of LC3-II abundance Statistical significance is relative to the mock condition unless otherwise indicated (**** = p value &lt; 0.0001, *** = p value &lt; 0.001, ** = p value &lt; 0.01, n.s. = non-significant, two-way ANOVA with Tukey's multiple comparison correction)."
NCBI gene IDs:
"altFUS: 
FUS: 2521"
Figure legend: "D-E, Quantification of FUS cytoplasmic granules, number (D) and area (μm2) (E) in cells over-expressing the bicistronic (+) or monocistronic (-) construct for FUS, FUS-G156E, FUS-R495x, FUS-K510E, FUS-Q519x, FUS-Q519I-fs527x, FUS-R521C, and FUS-P525L. Statistical comparisons are made between bicistronic and monocistronic versions of each construct (n=3 - biological replicates, mean ± SD, **** = p value &lt; 0.0001, *** = p value &lt; 0.001, ** = p value &lt; 0.01, * = p value &lt; 0.05, n.s. = non-significant, one-way ANOVA test with Sidak's multiple comparison)."
NCBI gene IDs:
"FUS: 2521"
Figure legend: "C-E, Locomotion assay represented by the percentage of climbing success in control and RU-486-treated transgenic Drosophila expressing mCherry or altFUS (C), the bicistronic FUS or the monocistronic FUS(Ø) (D), and the bicistronic FUS-R495x or the monocistronic FUS(Ø)-R495x (E) at day 1, 10 and 20 post-induction. Statistical comparisons were made between each population (n=4 - biological replicates). Indicated significance is between the monocistronic and the bicistronic transgenic flies of the RU-486-treated population (mean ± SD, n.s. = non-significant, * = p value &lt; 0.05, *** = p value &lt; 0.001, two-way ANOVA with Tukey's multiple comparison correction)."
NCBI gene IDs:
"altFUS: 
mCherry: 
FUS: 2521"
Figure legend: "A, Graphical representation of FUS synonymous mutations (yellow) found in ALS patients. The canonical FUS mRNA is represented in dark blue (ENST00000254108 or NM_004960). The FUS protein coding sequence is indicated in light blue, and the altFUS protein coding sequence is indicated in green. B, Images by confocal microscopy of TDP-43 (white), FUS (GFP tagged - green) and altFUS (Flag tagged - red) in HeLa cells over-expressing GFP-FUS(Flag), GFP-FUS(P31L-Flag)-S44=, GFP-FUS(A38V-Flag)-G51=, GFP-FUS(A46V-Flag)-G59=, GFP-FUS(R64P-Flag)-S77= (representative images from n=3). White arrows indicate some TDP-43 aggregates. The white scale bar corresponds to 10 μm. C, Quantification of cells with TDP-43 aggregates in HeLa cells (see panel B). The data are represented as the fold-change compared to the GFP-FUS(Flag) expressing cells. Statistical significance is indicated above the bars (n=3 - biological replicates, mean ± SD, **** = p value &lt; 0.0001, two-way ANOVA with Tukey's multiple comparison correction). "
NCBI gene IDs:
"Flag: 
GFP: 
FUS: 2521"
Figure legend: "A, B. Comparisons of RNAseq and ChIPseq data. Wt strain (A) and ∆rnjA (B). The x-axis shows the relative RNAP occupancy at a given gene (normalized ChIPseq coverage). The y-axis shows the relative abundance of the transcript (normalized RNAseq coverage; each dot is one gene). The genes are color-coded, ranging from yellow (low RNA abundance) to red (high RNA abundance) in wt. The ∆rnjA strain has higher RNAP occupancy mainly among the genes with less abundant transcripts. The color coding in (B) reveals no dramatic overall changes in the vertical direction (RNA abundance). If anything, some of the low abundance transcripts decreased further in level in the mutant strain. The main difference (mostly among the low abundance transcripts) is their shift in the horizontal direction to the right (towards higher occupancy with RNAP). Data represent mean values of three independent experiments."
NCBI gene IDs:
"rnjA: 939483"
Figure legend: "C. Relative expression of all sigma factors in wt (LK1371) versus ΔrnjA (LK1381). Wt levels of each sigma factor were set as 1 (indicated with the horizontal line)."
NCBI gene IDs:
"rnjA: 939483"
Figure legend: "D. Sigma-dependent genes and correlation with expression of sigma factors (significantly changed) in ΔrnjA (LK1381). The most down-regulated sigma factor was sigD and almost all sigD-dependent genes were downregulated. A similar trend is visible for the rest of sigma factors and their respective dependent genes. The x-axis shows expression of each sigma factor, the y-axis shows expression of genes for each sigma regulon. The violet (dark blue) line: regression line."
NCBI gene IDs:
"rnjA: 939483
sigD: 938482"
Figure legend: "Average gene profiles of normalized RNA-seq and RNAP ChIPseq coverages from wt (solid lines) and ∆rnjA (dashed lines) strains were plotted for gene classes I-IV (n = 762, 315, 553 and 1654 genes, respectively). Open reading frames were rescaled to 1 kb, upstream and downstream regions of 0.2 kb were also included in the plots. Data represent mean values of 3 independent experiments. The pie-chart shows the overall distribution of class I-IV and other genes."
NCBI gene IDs:
"rnjA: 939483"
Figure legend: "B. Relative expression (mRNA) of helD, mfd, rho, greA, nusA, nusB, and nusG in the ∆rnjA strain (normalized to wt [set as 1])."
NCBI gene IDs:
"greA: 937565
helD: 936037
mfd: 936653
nusA: 939628
nusB: 938606
nusG: 936851
rho: 937042
rnjA: 939483"
Figure legend: "(D) Representative fluorescence images of SCG neurons from neonatal mice (age P5-7) electroporated with plasmids coding for mCherry and for siRNA targeting STIM1. Neurons were cooled from 35°C to 7°C in the presence of 100μM verapamil. Electroporation was indicated by expression of mCherry (# in left-hand panel); in these neurons a cold response was absent (# in right-hand panel) while in neurons not expressing mCherry (*) cold responses were still observed. Scale bar 20μm."
NCBI gene IDs:
"mCherry: 
STIM1: 20866"
Figure legend: "(A) Representative TIRF microscopy images (imaging depth 100nm) showing that cooling from 35°C to 13°C increases ORAI1 puncta formation and colocalization of ORAI1 with STIM1 in HEK293 cells transfected with ORAI1-CFP and STIM1-YFP. Cooling to 13°C causes the appearance of new ORAI1 puncta (examples shown by *) that are not present in the 35°C image and are colocalised with STIM1. Cooling also causes STIM1 to move towards the cell surface in edge-on views (arrows). To reduce cell motility, the experiment was conducted in nominal 0[Ca]e, which potentially caused partial formation of STIM1 puncta before the images shown. Scale bar = 5μm. Temperature trace shown below. Mean fluorescence intensity adjusted to compensate for increase in intrinsic fluorescence of CFP and YFP caused by cooling (see Fig. S12)."
NCBI gene IDs:
"CFP: 
YFP: 
ORAI1: 84876
STIM1: 6786"
Figure legend: "Schematic representation of the upstream region of myogenin and amplified regions by RT-PCR (top). RT-PCR for the novel transcripts at the upstream region of myogenin in C2C12 myotubes (bottom). The presence or absence of reverse transcriptase (RT) is shown by (+) or (-), respectively. The templates (cDNA or genomic DNA) are indicated by C or G, respectively."
NCBI gene IDs:
"myogenin: 17928"
Figure legend: "The primers used for RT-PCR (top). Strand-specific RT-PCR for the novel transcripts at the upstream region of myogenin in C2C12 myotubes using total RNA (middle and bottom) and poly(A)+ RNA (bottom)."
NCBI gene IDs:
"myogenin: 17928"
Figure legend: "Chromatin pellet extract (CPE)/soluble nuclear extract (SNE) ratio of SRA1, Malat1, and Myoparr in C2C12 myotubes on a log scale. n = 3, mean ± SD."
NCBI gene IDs:
"Malat1: 72289
Myoparr: 114004354
SRA1: 24068"
Figure legend: "Quantitative RT-PCR for myogenin (A) and Myoparr (B) during myogenesis of C2C12 cells, primary mouse myoblasts (growth medium, GM), and mouse embryonic skeletal muscle (S.K.). The x-axis shows days after differentiation induction or embryonic days. n = 3, mean ± SD."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "qRT-PCR showing increased Myoparr expression by MyoD in C3H10T1/2 fibroblasts. n = 4, mean ± SD. **p &lt; 0.01 (unpaired two-tailed Welch's t-test)."
NCBI gene IDs:
"MyoD: 17927
Myoparr: 114004354"
Figure legend: "Treatment of recombinant TGF-β for 24 h decreased Myoparr expression in differentiating C2C12 cells. n = 3, mean ± SD. *p &lt; 0.05 (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Relative luciferase activities of the indicated promoter in differentiating C2C12 cells by exogenous MyoD. n = 3, mean ± SD. **p &lt; 0.01. (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"MyoD: 17927"
Figure legend: "C2C12 cells were transfected with 50 nM of indicated siRNAs in growth medium. After additional 24-h incubation in growth medium, cells were transferred to differentiation medium. The levels of Myoparr and myogenin expression were quantified by qRT-PCR 24 h after differentiation induction. n = 3, mean ± SD. *p &lt; 0.05. n.s., not significant. Statistical analyses were performed using unpaired two-tailed Student's t-test. In cases of unequal variances, unpaired two-tailed Welch's t-test was used."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "Western blot showing decreased expression of myogenin in differentiating C2C12 cells 48 h after Myoparr KD. Tubulin expression served as an internal control."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "The expression levels of myogenin and Myoparr evaluated by qRT-PCR in myogenin-depleted C2C12 cells. n = 3, mean ± SD. **p &lt; 0.01. ***p &lt; 0.001 (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "ChIP-qPCR detection of Pol II occupancy and histone modification status at the myogenin locus in Myoparr-depleted differentiating C2C12 cells. The data were normalized to input values. n = 3, mean ± SD. *p &lt; 0.05. **p &lt; 0.01 (unpaired two-tailed Student's t-test or unpaired two-tailed Welch's t-test)."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "Retrieval rate of Myoparr using Myoparr-ChIRP probes from C2C12 cells quantified by qPCR is shown as percent of input values. GAPDH was amplified as a negative control. n = 3, mean ± SD. ND, not detected."
NCBI gene IDs:
"GAPDH: 
Myoparr: 114004354"
Figure legend: "ChIRP-qPCR detection of the interaction between endogenous Myoparr and the myogenin promoter. The myogenin 3´UTR and GAPDH promoter were amplified as negative controls. The data were normalized to input values. n = 3, mean ± SD. *p &lt; 0.05. ** p &lt; 0.01 (unpaired two-tailed Welch's t-test). ND, not detected."
NCBI gene IDs:
"GAPDH: 
myogenin: 17928
Myoparr: 114004354"
Figure legend: "Immunocytochemistry for the detection of myogenin at 24 h (G) or MHC at 72 h (H) in Myoparr-depleted C2C12 cells after differentiation induction. Nuclei were counterstained with DAPI. Bar, 100 μm. The percentage of myogenin-positive cells or fusion index is shown as percent of the control. n = 3, mean ± SD. **p &lt; 0.01. ***p &lt; 0.001 (unpaired two-tailed Student's t-test or unpaired two-tailed Welch's t-test)."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Genes significantly differentially expressed in both Myoparr- and myogenin-depleted cells show correlated expression (R = 0.63, log 2 ratio scale)."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "Following immunoprecipitation, the interaction between Myoparr and endogenous Ddx17 was confirmed by immunoblotting using a Ddx17-specific antibody. Two different Ddx17 isoforms (p72 and p82) were observed."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Determination of the Ddx17-binding region of Myoparr by RNA pull-down analyses. Schematic diagram of full-length or truncated Myoparr used for RNA pull-down (top). In vitro transcribed/translated Ddx17 protein was pulled down by indicated Myoparr and then detected by western blot using a Ddx17 antibody (bottom)."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Relative luciferase activities of indicated constructs in differentiating C2C12 cells transfected with control or Myoparr shRNA (Myoparr KD)."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Significantly decreased MHC expression in Ddx17-depleted C2C12 cells is shown by immunocytochemistry. Nuclei were counterstained with DAPI. Bar, 100 μm. Fusion index is shown as percent of the control. n = 3, mean ± SD. **p &lt; 0.01 (unpaired two-tailed Student's t-test). Immunocytochemistry for myogenin in Ddx17-depleted C2C12 cells. Bar, 100 μm. Myogenin-positive cells are shown as percent of the control. n = 4, mean ± SD. **p &lt; 0.01 (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"Ddx17: 67040"
Figure legend: "Relative luciferase activities of the -1650-Luc by the combination of MyoD and Ddx17 or Ddx17 mutant (K142R). Bars indicate the average of two independent experiments, and open circles represent the values of each experiment."
NCBI gene IDs:
"Ddx17: 67040
MyoD: 17927"
Figure legend: "Ddx17 or Ddx17 mutant (K142R) was pulled down by full-length Myoparr and then detected by western blot."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Reduced interaction between endogenous Ddx17 and PCAF in Myoparr-depleted C2C12 cells. After Myoparr KD, the cell lysates were subjected to immunoprecipitation (IP) with a Ddx17-specific antibody 36 h after differentiation induction (right panel). Each lysate was loaded as an input (left panel). Relative quantification of (E) is shown as IP/input ratio. n = 3, mean ± SD. *p &lt; 0.05. n.s., not significant (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"Myoparr: 114004354"