EMBO/SourceData huggingface dataset:
Figure legend: "A-C. Monitoring of mammary gland development following transplantation of wt or Sharpincpdm mouse mammary epithelium in the mammary fat pads (epithelium-free) of virgin wt mice. A. Representative carmine-alum stained images of mouse mammary glands generated from wt or Sharpincpdm epithelial tissue transplants. B. Quantification of transplant growth take-on-rate (n = 17). C. Quantification of fat pad filling rate of grown transplants (n = 7 - 10) in wt hosts."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "D. Monitoring of mammary gland differentiation during pregnancy following transplantation of wt or Sharpincpdm mouse mammary epithelium into wt hosts. Host animals were subsequently mated and mammary glands isolated at P15. Representative Carmine-alum stained mammary gland whole mounts generated from wt and cdpm mammary epithelial transplants (upper panel), and magnifications of the branched ductal epithelium (lower panel) are shown (n = 5 mice). Scale bars represent 1 mm."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "E. Representative images of carmine-alum stained mouse mammary gland whole mounts generated from 7 weeks old S100a4-Cre; Sharpinfl/fl conditional knockout mice and their littermate controls (S100a4-Cre; Sharpin fl/+) F. Quantification of the normalized Sharpincpdm and S100a4-Cre; Sharpinfl/fl mammary ductal outgrowth area relative to littermate control mice (wt and Sharpincpdm n = 10 glands; S100a4-Cre; Sharpin fl/+ and S100a4-Cre; Sharpinfl/fl n = 8 glands). G. Quantification of the number of TEBs per gland (n = 8 - 9 glands). Mean ± SEM."
NCBI gene IDs:
"Cre: 
S100a4: 20198
Sharpin: 106025"
Figure legend: "A. Second harmonic generation (SHG) and unfiltered multiphoton (MP) imaging of collagen fibres surrounding TEBs (dashed line) in 6-7 weeks old wt and Sharpincpdm mouse mammary glands (n = 6 - 9 mice, 5 - 13 TEBs analysed per mouse; scale bars represent 100 µm). Magnified images of collagen fibres are also shown (scale bars represent 50 µm). B. Frequency of clear collagen bundles around TEBs per mouse (Mean ± SEM, n = 6 - 9 mice)."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "C. AFM indentation analysis of stromal stiffness in 6-7 weeks old wt and Sharpincpdm mouse mammary gland tissue sections (n = 3 mice; 3 - 6 sample sections per mouse and 192 indentation curves per sample)."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "A. Ingenuity Pathway Analysis of differential gene expression (fold-change (FC) &gt; 1.5, p &lt; 0.05) in wt and Sharpincpdm MSFs (passage 0). The differentially expressed genes implicated in the most significantly altered canonical pathways (leukocyte adhesion and transmigration, and tissue fibrosis) are shown. Three independent cell isolation replicates were compared pairwise. FC colour scale represents a range from 0−4."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "C-D. Collagen plug contraction assay using wt and Sharpincpdm MSFs or wt MSFs with SHARPIN siRNA silencing C. Representative light microscopy images of the collagen plugs three days post cell culture. D. Quantification of the average collagen plug area 1−3 days after seeding (n = 3-6). Scale bars represent 2 mm."
NCBI gene IDs:
"SHARPIN: 106025
Sharpin: 106025"
Figure legend: "E. SHARPIN protein level after silencing with negative control siRNA or SHARPIN siRNA in wt MSFs. Tubulin was used as loading control."
NCBI gene IDs:
"SHARPIN: 106025"
Figure legend: "F-I. Traction force microscopy analysis of wt and Sharpincpdm MSFs on collagen-coated matrices. F. Representative images of traction forces observed in wt and Sharpincpdm cells (colour scale units: Pa; cell boundaries are indicated by dashed lines). G. Quantification of strain energy per cell (n = 8 − 11 cells from two independent experiments)."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "F-I. Traction force microscopy analysis of wt and Sharpincpdm MSFs on collagen-coated matrices. H. Representative images of the traction forces and mCherry fluorescence of mCherry-Ctrl transfected wt cells and mCherry-Ctrl or mCherry-SHARPIN transfected Sharpincpdm cells. Scale bars represent 20 µm. I. Quantification of strain energy per cell (wt mCherry control n = 26; Sharpincpdm mCherry-Ctrl n = 21; Sharpincpdm mCherry-SHARPIN n = 24 cells from five independent experiments)."
NCBI gene IDs:
"Sharpin: 106025
SHARPIN: 106025"
Figure legend: "J-K. Focal adhesion dynamics. J. Visualization of GFP-Paxillin dynamics in a wt and a Sharpincpdm cell over time. Colour scale represents a range from early to late occurring focal adhesions. K. Quantification of focal adhesion average size, assembly rate and disassembly rate (wt n =17527, 5250, 5311 FAs; and Sharpincpdm n= 9052, 2790, 3264 FAs, respectively from two independent experiments). Mean ± SEM."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "A. Quantification of soluble collagen levels in wt and Sharpincpdm MSF cultures on days 2, 5 and 7 post serum removal (n = 6 from two independent experiments)."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "B-C. Analysis of the collagen network in cell-derived matrices (CDMs) produced by wt and Sharpincpdm MSFs using COL1A1 immunolabelling. B. Maximum intensity projections of confocal images are shown. Scale bars represent 50 μm. C. Quantification of distinct collagen fibre bundles in wt and Sharpincpdm CDM samples (n = 5). Mean ± SEM."
NCBI gene IDs:
"Sharpin: 106025"
Figure legend: "Representative confocal images of cell-type-specific promoter::GFP:AGO1 translational fusions."
NCBI gene IDs:
"AGO1: 
GFP: "
Figure legend: "Left: input and IPed AGO1 following post-lysis addition of a radiolabeled siRNA duplex (siGFP). Right: northern analysis of AGO1-bound sRNAs."
NCBI gene IDs:
"GFP: "
Figure legend: "Representative confocal images of cell-type-specific promoter::GFP transcriptional fusions in hyl1 vs WT roots."
NCBI gene IDs:
"hyl1: 837498"
Figure legend: "Hierarchical clustering of validated miRNA targets between different cell layers in either WT or hyl1 roots."
NCBI gene IDs:
"hyl1: 837498"
Figure legend: "qRT-PCR validation of three distinct classes of direct miRNA target transcripts from total root-, total root polysome- or epidermis-specific polysome-associated RNA. The AT2G37050 kinase is positively regulated by LAC17. Scale bars: 50μM. All qPCR values were normalized to the internal control ACTIN2."
NCBI gene IDs:
"ACTIN2: 
AT2G37050 kinase: 818281
LAC17: 836124"
Figure legend: "miRoot snapshots of whole-root (left) and layer-specific (right) LAC17 mRNA accumulation in WT versus hyl1, as assessed by polysome profiling."
NCBI gene IDs:
"hyl1: 837498
LAC17: 836124"
Figure legend: "miRoot snapshot of cell-type specific miRNAs showing, in this case, a sum group of the miR156 family. The right panel shows the gradient of expression revealed upon removal of the stele (blue box)."
NCBI gene IDs:
"miR156: 28718130///28716731///5008308///5007886///28721198///3770614///28721147///28720200///28720191///28718303"
Figure legend: "Snapshot of the SPL6 miR156 translatome in WT and hyl1 backgrounds. Removal of the stele (blue box) reveals subtleties of miR156-mediated gene regulation in the other layers, resulting in a near-mirror image of (A)."
NCBI gene IDs:
"SPL6: 843248
hyl1: 837498
miR156: 28718130///28716731///5008308///5007886///28721198///3770614///28721147///28720200///28720191///28718303"
Figure legend: "Confocal image of pmiR396a::H2B:GFP. Normalized read counts from AGO1-IP-Seq and independent northern analysis of AGO1-loaded miR396."
NCBI gene IDs:
"GFP: 
H2B: 
miR396: 5007870
miR396a: 5007870"
Figure legend: "Representative confocal image of the pGRF2::H2B:GFP translational fusion."
NCBI gene IDs:
"GFP: 
H2B: 
GRF2: 844165"
Figure legend: "Focused miRoot analysis of the GRF2 translatome signal in WT versus hyl1 columella. Graph: GRF2 normalized read counts."
NCBI gene IDs:
"GRF2: 844165
hyl1: 837498"
Figure legend: "Representative confocal images of the pGRF2::GRF2:GFP translational fusion in WT or hyl1 backgrounds."
NCBI gene IDs:
"GFP: 
GRF2: 844165
hyl1: 837498"
Figure legend: "Focused miRoot analysis of the PLT1 translatome signal in WT versus hyl1 columella."
NCBI gene IDs:
"hyl1: 837498
PLT1: 821632"
Figure legend: "Layer-specific normalized read counts of miR169 paralogs (separated into 4 different groups based on sequence identity: a, b-c, d-g, h-n) from AGO1-IP-deep-Seq and independent northern analysis, as in Fig.1F."
NCBI gene IDs:
"miR169: 5008036///5008035///5008034///5008033///5008032///5008031///5007706///5008152///5008003///5007800///5007801///5008285///5008226///28719269"
Figure legend: "Cell-type specific AGO1-IP signals for each miR169 sequence-group with (upper) or without (lower panel) the miRoot scale normalization option."
NCBI gene IDs:
"miR169: 5008033///5008032///5008031///5008152///5008003///5008285///5008036///5008035///5008034///5007706///5007800///5007801///5008226///28719269"
Figure legend: "Predicted base-pairing of indicated miR169 sequence-group to four NF-YA target transcripts. Pairing with expectation value greater than 3 was not considered. The color gradient (pale: low, dark: strong) qualitatively approximates the pairing contribution of each miR169 sequence-group according to the upper panel of (B). miRoot snapshots of layer-specific translatome signals of four out-of-seven miR169 NF-YA targets in WT versus hyl1 backgrounds."
NCBI gene IDs:
"hyl1: 837498
miR169: 5007800///5008035///5008033///5008003///5008226///5007706///5008034///5008036///5008032///5008031///5008152///5007801///5008285///28719269
NF-YA: 821640///841856///843614///819738"
Figure legend: "Browser representation and independent northern analysis and AGO1-loaded miR165/166."
NCBI gene IDs:
"miR165: 3766638
166: 3771553///3771422///3771398///3770612///3770611///3769765///28718357"
Figure legend: "miRoot snapshot of PHB translatome signals in WT versus hyl1 backgrounds."
NCBI gene IDs:
"hyl1: 837498
PHB: 818036"
Figure legend: "Representative confocal images of pmiR395(a/c/e)::H2B:GFP transcriptional fusions under SO4--deficient or SO4--proficient growth conditions. Northern analysis of AGO1-loaded miR395 under the conditions in (C)."
NCBI gene IDs:
"GFP: 
H2B: 
miR395: 5007844///5007729///5007727"
Figure legend: "Representative confocal images of the pAPS4::APS4:GFP translational fusion under the conditions in (C)."
NCBI gene IDs:
"GFP: 
APS4: 834400"
Figure legend: "Representative confocal image of the pSHR::GFP:P19 translational fusion and (B) principle of P19-based miRNA duplex sequestration."
NCBI gene IDs:
"GFP: 
P19: 
SHR: 829919"
Figure legend: "Binding of miR395 by SHR::HA:P19 under SO4--sufficient (+) and -deficient (-) growth conditions."
NCBI gene IDs:
"HA: 
P19: 
miR395: 5007727///5007729///5007844
SHR: 829919"
Figure legend: "qRT-PCR analysis of polysome-associated APS4 in the cortex or epidermis in the presence (+) or absence (-) of SHR::HA:P19. Signals are relative to SHR::HA:P19 (-) lines given a value of 1."
NCBI gene IDs:
"HA: 
P19: 
APS4: 834400
SHR: 829919"
Figure legend: "Representative confocal image of the pmiR397b::H2B:GFP transcriptional fusion (left) and the browser AGO1-loaded signal for miR397b (right)."
NCBI gene IDs:
"GFP: 
H2B: 
miR397b: 5008141"
Figure legend: "miR160 northern analysis in whole-leaf, vasculature and epidermis. U6: loading control."
NCBI gene IDs:
"U6: 
miR160: 3771452///3770127///3768181"
Figure legend: "Western analysis of the promoter::H2B:GFP translational fusions shown in (5C) in the cell types isolated in (G)."
NCBI gene IDs:
"GFP: 
H2B: "
Figure legend: "qRT-PCR analysis of ARF17 in whole-leaves or in separated cell types in WT versus hyl1 backgrounds."
NCBI gene IDs:
"ARF17: 844120
hyl1: 837498"
Figure legend: "A Immunoblot of PPAR-α and Tubulin (control) (top) in WAT from WT and FADD-D mice with relative quantification (bottom). Data are expressed as mean ± SEM from three independent experiments. *P = 0.0035 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B Immunostaining of PPAR-α expression in WAT from WT and FADD-D mice (Scale bars = 50 μm). Shown are typical results from four different fields and three different experiments."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "C Quantitative real time PCR for PPAR-α and PPAR-α target genes, using RNA from epididymalfat from 10-week-old male WT and FADD-D mice fed a SD. Data are expressed as mean ± SEM from two independent experiments (n = 6 total for each genotype). *P = 0.0022, **P = 0.0083, ***P = 0.0107, #P = 0.0011, ##P = 0.0037 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082
PPAR-α: 19013"
Figure legend: "D ChIP assay of CPT1b promoter in adipocytes isolated from WT, FADD-D and ad-FADDmice. Soluble chromatin from adipocytes was immunoprecipitated with control mouse IgG or antibodies against PPAR-α. The experiments were performed in triplicate."
NCBI gene IDs:
"CPT1b: 12895
FADD: 14082"
Figure legend: "E ChIP assay of CPT1b promoter in adipocytes isolated from WT and FADD-D mice. Soluble chromatin from adipocytes was immunoprecipitated with control mouse IgG or antibodies against FADD. The experiments were performed in triplicate."
NCBI gene IDs:
"CPT1b: 12895
FADD: 14082"
Figure legend: "F PPAR-α and RIP140coimmunoprecipitate in WT but not FADD knockout or FADD-D MEFs. PPAR-α was immunoprecipitated (IP) from the cell lysates of WT or FADD knockout MEFs. Data shown are representative of three independent experiments having similar results."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "G PPAR-α and RIP140coimmunoprecipitate in WT but not FADD knockout MEFs upon WY-14,643 stimulation. Ligand-dependent interactions were examined in the presence of 100 μM WY-14,643 where indicated. Data shown are representative of three independent experiments having similar results."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "H Coimmunoprecipitation analysis of FADD and RIP140 in 293T cells. As indicated, lysates from the cells transfected with plasmids encoding Flag-RIP140, HA-FADD-A or HA-FADD-D were subjected to immunoprecipitation with an anti-Flag antibody. FADD-A and FADD-D were detected using an anti-HA antibody. Data shown are representative of three independent experiments having similar results."
NCBI gene IDs:
"FADD: 8772"
Figure legend: "I Coimmunoprecipitation analysis of FADD and PPAR-α in 293T cells. As indicated, lysates from the cells transfected with plasmids encoding Flag-FADD-A, Flag-FADD-D or HA-PPAR-α were subjected to immunoprecipitation with an anti-Flag antibody. PPAR-α was visualized by anti-HA. Data shown are representative of three independent experiments having similar results."
NCBI gene IDs:
"FADD: 8772"
Figure legend: "A Left, Body weights of male WT and FADD-D mice on either a SD (n = 8 for each genotype) or a HFD (n = 6 for each genotype). Right, Food intake per mouse fed a HFD measured over 20 days normalized by body weight (n = 10 for each genotype). Results are means ± SEM. *P = 0.0007 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B Representative photographs of fat pads and organs of 10-week-old male FADD-D and WT mice."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "C Left, fat pad weights as a percentage of body weight (BW) and in absolute amounts (inset) from male FADD-D and WT mice. Mice were fed a SD or a HFD until 15 weeks of age (n = 6 for each genotype). Results are means ± SEM. *P = 0.0012, **P = 0.0002, ***P = 0.0027, ****P = 0.0014, ^P = 0.0038, ^^P = 0.0019, #P = 0.0045, ##P = 0.0007, *^P = 0.0252, *^^P = 0.0082, ###P = 0.0275, ####P = 0.0071 (one-way ANOVA). Middle, fat pad weights of male FADD-D and WT mice on a HFD at 30 weeks of age (n = 6 for each genotype). Inset, triacylglycerol (TAG) content in epididymal WAT. Data are expressed as means ± SEM. *P = 0.0003, **P = 0.0005, ***P = 0.0019, ****P = 0.0011 (Student's t-test). Right, weight of liver normalized by body weight for male FADD-D and WT littermates at the age of 15 weeks (n = 6 for each genotype). Data are expressed as means ± SEM. NS, not statistically significant (Student's t-test). Epi, epididymal fat; Ren, renal fat; Ing, inguinal fat."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "F Paraffin-embedded sections of WAT and BAT from WT and FADD-D mice were stained with H&amp;amp;amp;E. Scale bar = 50 μm. Shown are typical results from four different fields and three different experiments."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "G Left, relative amounts of liver and muscle triacylglycerol contents in 10-month-old WT and FADD-D mice (n = 6 for each genotype). Right, relative amounts of cholesterol in the liver and muscle of the same group of WT and FADD-D mice (n = 6 for each genotype). Data are expressed as means ± SEM. *P = 0.0015, **P = 0.0027, ***P = 0.0318 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "H Left, plasma triacylglycerol levels in 10-month-old WT and FADD-D mice following fasting for 20 h (n = 5 for each genotype). Right, plasma cholesterol levels in the same group of WT and FADD-D mice (n = 5 for each genotype). Data are expressed as means ± SEM. *P = 0.0124 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A Serum free fatty acid (FFA) levels and triacylglycerol levels in WT and FADD-D mice fed a HFD for 20 days (n = 5 for each genotype). Data are expressed as means ± SEM. *P = 0.0059, **P = 0.0012 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B Serum levels of leptin in WT and FADD-D mice (n = 4 for each genotype) fed a SD or a HFD. Data are expressed as means ± SEM. *P = 0.0032, **P = 0.0003, ***P = 0.0038 (one-way ANOVA)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "C Morphology of inguinal WAT, BAT, and liver from WT and FADD-D mice fed a HFD for 20 days. WAT and BAT were stained with hematoxylin and eosin. Liver tissues were stained with Oil red O to demonstrate lipid accumulation and counterstained with hematoxylin. Scale bars = 50 μm. Shown are typical results from four different fields and three different experiments."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "D Comparison of cell size in WAT of WT and FADD-D mice maintained on SD and HFD (n = 5 for each genotype)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "E FADD-D mice lost more weight as compared with WT on 20-h fasting (n = 4 for each genotype). Data are expressed as means ± SEM. *P = 0.0085 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "F Whole-body oxygen consumption rate (VO2) during 24 hr in WT and FADD-D mice fed a SD (n = 4 for each genotype). Data are expressed as means ± SEM. *P = 0.0055, **P = 0.0081 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "G Average respiratory exchange ratio (VCO2/VO2) (RER) for the dark and light period in WT and FADD-D mice (n = 5 for each genotype). Data are expressed as means ± SEM. *P = 0.0042, **P = 0.0079 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "I Average values of energy expenditure (EE) for the 24 hr period in WT and FADD-D mice (n = 5 for each genotype). Data are expressed as means ± SEM. *P = 0.0054 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "J Physical activity for the dark and light period in WT and FADD-D mice (n = 5 for each genotype). Data are expressed as means ± SEM. NS, not statistically significant (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A Left, transmission electron microscopy analysis of adipocytes from WAT and BAT of WT and FADD-D mice. Arrowheads indicate mitochondria and lipid droplets (L) (× 5,000 magnification). Right, quantification of mitochondria in WAT and BAT of WT and FADD-D mice, respectively. Data are expressed as mean ± SEM from three independent experiments (n = 6 total for each genotype). *P = 0.0007, **P = 0.0083 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B Quantitative real time PCR for UCP1, Dio2, PPARd, UCP3, CPT1 and PGC1-α, using RNA from epididymalfat from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from three independent experiments (n = 6 total for each genotype). *P = 0.0014, **P = 0.0025, ***P = 0.0006, ****P = 0.0007, #P = 0.0003, ##P = 0.0008 (Student's t-test)."
NCBI gene IDs:
"CPT1: 12895///12894
Dio2: 13371
FADD: 14082
PPARd: 19015
PGC1-α: 19017
UCP1: 22227
UCP3: 22229"
Figure legend: "C Quantitative real time PCR for UCP3, CPT1, PPARd and PGC1-α, using RNA from skeletal muscle from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from three independent experiments (n = 6 total for each genotype). *P = 0.0027, **P = 0.0329, ***P = 0.0092, ****P = 0.0085 (Student's t-test)."
NCBI gene IDs:
"CPT1: 12895///12894
FADD: 14082
PPARd: 19015
PGC1-α: 19017
UCP3: 22229"
Figure legend: "D Quantitative real time PCR for UCP1, using RNA from BAT from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from three independent experiments (n = 6 total for each genotype). *P = 0.0018 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082
UCP1: 22227"
Figure legend: "E Immunoblotting of UCP1 and Tubulin (control) (top) with relative quantification (bottom), using total cell lysates of BAT from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from three independent experiments. *P = 0.0217 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "F Oxygen consumption in isolated BAT from 10-week-old male WT and FADD-D mice on a SD (n = 4 for each genotype). Data are expressed as mean ± SEM. *P = 0.0025, **P = 0.0006 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "G Quantitative real time PCR for PGC1α, C/EBPα and PPARγ, using RNA from BAT from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from two independent experiments (n = 4 total for each genotype). *P = 0.0113 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"C/EBPα: 12606
FADD: 14082
PPARγ: 19016
PGC1α: 19017"
Figure legend: "H β-oxidation analysis of white adipocytes isolated from WT (n = 5) and FADD-D mice (n = 4). 1-14C labeled oleic acid was added to the medium containing isolated adipocytes (5×105), and 14CO2 trapped by the filter paper soaked with hyamine hydroxide was measured after a 3 h-incubation by a scintillation counter. Data are expressed as mean ± SEM. *P = 0.0022 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "I Basal and stimulated (+ 100 nM isoproterenol) lipolysis, as measured by glycerol (left) and fatty acids (right) released from explants of epididymal WAT from overnight fasted 10-week-old male WT and FADD-D mice fed a HFD (n = 4 for each genotype). Data are expressed as mean ± SEM. *P = 0.0078, **P = 0.0065, ***P = 0.0008, ^P = 0.0133, ^^P = 0.0053, ^^^P = 0.0006, ^^^^P = 0.0001, #P = 0.0082, ##P = 0.0051, ###P = 0.0009, *^P = 0.0151, *^^P = 0.0019, **^^P = 0.0005, ***^P = 0.0002 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A Quantitative real time PCR for enzymes related to lipid metabolism, using RNA from epididymalfat from 10-week-old male WT and FADD-D mice on a SD. Data are expressed as mean ± SEM from two independent experiments (n = 5 total for each genotype). *P = 0.0162, **P = 0.0105, ***P = 0.0063 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B cAMP abundance in epididymalWAT from 10-week-old male WT and FADD-D mice on a HFD (n = 4 for each genotype). Data are expressed as mean ± SEM. *P = 0.0033 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "C Basal and forskolin-stimulated adenylyl cyclase activity in epididymalWAT from 10-week-old male WT and FADD-D mice on a HFD (n = 4 for each genotype). Data are expressed as mean ± SEM. *P = 0.0177, *P = 0.0091 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "E Basal and stimulated (+200 nM isoproterenol) lipolysis in epididymalWAT of 10-week-old male WT and FADD-D mice (n = 4 for each genotype) treated with or without 10 μM BAY. Data are expressed as mean ± SEM. *P = 0.0019, **P = 00038, ^P = 0.0035, ^^P = 0.0053 (one-way ANOVA). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"