EMBO/SourceData huggingface dataset:
Figure legend: "splenocytes from 1-year-old WT and Ythdf2 cKO mice were analyzed by flow cytometry (n=4). C, Representative plots (left) and the percentage (right) of naïve (CD62LhiCD44lo), central memory (cMem, CD62LhiCD44hi), and effector memory (eMem, CD62LloCD44hi) T cells. Data are presented as means ± s.d. and were analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "splenocytes from 1-year-old WT and Ythdf2 cKO mice were analyzed by flow cytometry (n=4). D, Representative plot (left) of FOXP3 expression level and the FOXP3 expression MFI (right) in splenic Treg cells. Data are presented as means ± s.d. and were analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "splenocytes from 1-year-old WT and Ythdf2 cKO mice were analyzed by flow cytometry (n=4). E, Representative plots (left) and percentage (right) of FOXP3 expressing Treg cells in CD4 T cells. Data are presented as means ± s.d. and were analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "splenocytes from 1-year-old WT and Ythdf2 cKO mice were analyzed by flow cytometry (n=4). F, Representative plot (left) of FOXP3 Treg cells PD-1 expression and the MFI value (right) in WT and Ythdf2 cKO Treg cells. Data are presented as means ± s.d. and were analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "B, Functional assay with WT (n=5) or Ythdf2 cKO Treg (n=5) cells isolated from B16F10 tumors on day 16. B, Percentage of IFNγ producing CD8+ T cells after coculture. Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "G, Representative plots (left) and ratio (right) of GFP+/GFP- Treg cells in B16F10 tumors from Foxp3CreGFP/+Ythdf2fl/+ or Foxp3CreGFP/+Ythdf2fl/fl mice."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Foxp3: 20371
Ythdf2: 213541"
Figure legend: "E, IGV visualization showing m6A and YTHDF2 binding at Nlrc3, Nfkbie, and Traf3 loci in CD4+ T cells (GSE188853)."
NCBI gene IDs:
"Nfkbie: 18037
Nlrc3: 268857
Traf3: 22031"
Figure legend: "E, Relative mRNA levels of indicated genes in WT or Ythdf2 cKO Treg cells before or after 24 hours TNFα stimulation. Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "F, WT or Ythdf2 cKO Treg cells from the spleen were stimulated with 10 ng/mL TNFα in vitro. 24 hours post TNFα stimulation, cells were treated with Actinomycin D. Decay of Nlrc3, Nfkbie, and Traf3 was monitored by RT-qPCR (n=3, biological replicates). Data are presented as means ± s.d. and analyzed two-way ANOVA."
NCBI gene IDs:
"Nfkbie: 18037
Nlrc3: 268857
Traf3: 22031
Ythdf2: 213541"
Figure legend: "H, Functional assay with WT (n=4) or Ythdf2 cKO Treg (n=5) cells isolated from B16F10 tumors on day 16. H, Percentage of IFNγ producing CD8 T cells after coculture. Each data point represents one mouse. Data are presented as means ± s.d. and analyzed by two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Ythdf2: 213541"
Figure legend: "B Concentration-dependent avoidance of saponin in control, Gr28bMi and Gr28b Df/ Gr28bMi (n ≥ 4)."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "D, E PER assay for control and Gr28bMi flies in the (D) legs or (E) proboscis. Flies were initially given 2% sucrose, then 2% sucrose in combination with 5% saponin (n = 4). The error bars represent SEMs. The asterisks indicate significant differences from that of the control detected by a single-factor ANOVA with Scheffe's analysis as a post hoc test to compare two sets of data (*P &lt; 0.05, **P &lt; 0.01). "
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "A Mapping analysis of all the sensilla following stimulation with 5% saponin in control and Gr28bMi flies (we followed Tanimura's nomenclature) (n ≥ 8). The error bars represent SEMs. The asterisks indicate significant differences from that of the control detected by a single-factor ANOVA with Scheffe's analysis to compare two sets of data (*P &lt; 0.05, **P &lt; 0.01)."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "C Tip recording analysis of S6 sensilla from control and Gr28bMi flies. Stimuli used are 0.2% DEET, 1.0 mM coumarin, 0.5 mM quinine, 10 mM umbelliferone, 10 mM caffeine, 0.3 mM strychnine, 0.3 mM lobeline, 0.1 mM chloroquine, 0.2 mM denatonium, 0.5 mM papaverine, 0.1 mM berberine and 0.1% SDS (n ≥ 7). The error bars represent SEMs."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "D Binary food choice assays were conducted after GRNs were ablated by expressing the cell death gene (hid), under Gr33a-GAL4 or Gr66a-GAL4. All heterozygote controls (UAS-hid/+, Gr33a-GAL4/+, and Gr66a-GAL4/+) are shown (n ≥ 4). The error bars represent SEMs. The asterisks indicate significant differences from that of the control detected by a single-factor ANOVA with Scheffe's analysis to compare two sets of data (*P &lt; 0.05, **P &lt; 0.01)."
NCBI gene IDs:
"GAL4: 855828
Gr33a: 34641
Gr66a: 38935
hid: 40009"
Figure legend: "B RT-PCR analysis with the indicated primers in A for control and Gr28bMi flies with tubulin as a control. From left to right, the band of tubulin of wild-type, tubulin of Gr28bMi, DNA ladder, Gr28b of wild-type, and Gr28b of Gr28bMi."
NCBI gene IDs:
"tubulin: 
Gr28b: 117496"
Figure legend: "Each isoform from Gr28b.a to Gr28b.e was driven by Gr28b.a-GAL4 or Gr28b.c-GAL4 in a Gr28bMi background. The asterisks indicate significant differences from that of the Gr28b mutant detected by a single-factor ANOVA with Scheffe's analysis (**P &lt; 0.01). C Binary food choice assay for recovery experiments with each of the five alternative Gr28b isoforms using the GAL4/UAS systems. (n ≥ 4)."
NCBI gene IDs:
"GAL4: 855828
Gr28b.a: 117496
Gr28b: 117496
Gr28b.c: 117496
Gr28b.e: 117496"
Figure legend: "D Each isoform from Gr28b.a to Gr28b.e was driven by Gr28b.a-GAL4 or Gr28b.c-GAL4 in a Gr28bMi background. The asterisks indicate significant differences from that of the Gr28b mutant detected by a single-factor ANOVA with Scheffe's analysis (**P &lt; 0.01). D Saponin-induced action potential frequencies from the indicated transgenic flies on S6 sensilla (n = 7)."
NCBI gene IDs:
"GAL4: 855828
Gr28b.a: 117496
Gr28b: 117496
Gr28b.c: 117496
Gr28b.e: 117496"
Figure legend: "A Binary food choice assays were conducted after GRNs were ablated by expressing the cell death gene (hid), under Gr28b.c-GAL4 (n = 4)."
NCBI gene IDs:
"GAL4: 855828
Gr28b.c: 117496
hid: 40009"
Figure legend: "A, B Survival rate of (A) control and (B) Gr28bMi adult raised on control and the indicated concentrations of saponin-containing cornmeal diet (n = 4)."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "C, D Survival rate based on binary food choice. control and Gr28bMi were allowed to feed 100 mM fructose versus 100 mM (C) or 200 mM fructose (D) mixed with 5% or 7.5% saponin."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "E, F Cumulative pupation rate of (E) control and (F) Gr28bMi larvae raised on the indicated saponin-containing food as well as control food (n = 4). The error bars represent SEMs. The asterisks indicate significant differences from normal diet fed which were detected by a single-factor ANOVA with Scheffe's analysis (*P &lt; 0.05, **P &lt; 0.01)."
NCBI gene IDs:
"Gr28b: 117496"
Figure legend: "C.  Western blot analysis of CTRL#1 or BRD9-KO#1 lysates from cells treated for the indicated  times  with 1000 IU/mL of IFN-α2. The indicated proteins were detected with specific antibodies. Data are representative of at least two biological replicates."
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "D. Immunofluorescence  analysis of CTRL#1 or  BRD9-KO#1 cells treated for the indicated  times  with 1000 IU/mL of IFN-α2. The indicated proteins were detected with specific antibodies. Data are representative of at least two biological replicates. Scale bars indicate 10μm."
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "F. Western blot  analysis of CTRL#1 or BRD9-KO#1 lysates from cells treated or not for 16h with 1000 IU/mL of IFN-α2. The indicated proteins were detected with specific antibodies. Data are representative of at least two biological replicates."
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "I. CTRL#1 or BRD9-KO#1 cells were stably-transduced with  BRD9-expressing, or control (EV, empty vector),  lentiviruses  and treated with 1000 IU/mL of IFN-α2  for 16h prior to infection with  IAV (WSN/33) at an MOI of 0.01 PFU/cell. Viral titers  were determined after 24h by  plaque assay. Data information: data represent means and standard deviations from n=3 biological experiments (individual data points shown). Statistical significance was determined by 1-way ANOVA on log-transformed plaque counts (**p-value &lt; 0.001; ****p-value &lt; 0.0001). Dotted lines are a visual guide for maximum and minimum virus replication in control cells in the absence and presence of  IFN-α2, respectively. Numbers above  IFN-α2-treated bars indicate their approximate difference to the respective untreated conditions."
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "F.  RT-qPCR confirmation of transcriptomics results for selected transcripts (MX1,  MX2, IFITM1, ISG15 and IFIT1) in A549 cells treated as outlined in (A). GAPDH transcript levels were used for normalization. Data represent means and standard deviations from n=3 biological experiments (individual data points shown). Statistical significance was determined by unpaired 2-tailed t-test on ΔCt values (*p-value &lt; 0.05; n.s. not significant)."
NCBI gene IDs:
"GAPDH: 2597
IFIT1: 3434
IFITM1: 8519
ISG15: 9636
MX1: 4599
MX2: 4600"
Figure legend: "G.  Western blot  analysis of CTRL#3 or BRD9-KO#3 cells stably transduced with lentiviruses expressing empty vector (EV), or wt and chimeric constructs"
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "H.  Western blot  analysis of input lysates and anti-BRD9 immunoprecipitations (IPs) from cells described in (G) expressing wt BRD9 or the chimeric BRD7DUF. The indicated proteins were detected with specific antibodies. Data are representative of two biological replicates."
NCBI gene IDs:
"BRD7: 29117
BRD9: 65980"
Figure legend: "I. The various CTRL#3 or  BRD9-KO#3 cells described in (G) were stimulated and infected as described in (B). Data information: data represent means and standard deviations from n=3 biological experiments (individual data points shown). Numbers above IFN-α2-treated bars indicate their approximate difference to the respective untreated conditions. Dotted lines are a visual guide for minimum virus replication in control cells in the presence of IFN-α2. Statistical significance was determined by 1-way ANOVA on log-transformed intensity values (***p-value &lt; 0.001; ****p-value &lt; 0.0001; n.s. not significant)."
NCBI gene IDs:
"BRD9: 65980"
Figure legend: "A) (left) t-sne plot from scRNAseq data of human organoids and cluster assignment of colonic cell populations. Red color corresponds to undifferentiated cells, light green corresponds to surface colonocytes (enterocytes). t-sne plot for selected genes from single cell RNAseq of human colon epithelial cells revealing expression of ACE2 specifically in differentiated surface colonocytes expressing KRT20 and AQP8, which also express the IFN-γ receptor IFNGR2. B) Immunofluorescence staining for ACE2 (red) and KRT20 (green) of human colon tissue section indicating ACE2 expression in surface colonocytes. Scale bar: 50 µm."
NCBI gene IDs:
"ACE2: 59272
AQP8: 343
IFN-γ: 3458
IFNGR2: 3460
KRT20: 54474"
Figure legend: "Comparison of E) KRT20, F) MUC2, G) CHGA, H) LGR5 mRNA expression in organoids grown in FM or in basic medium and either untreated or treated with IFN-γ (n=3). Data are presented as mean +/- SD ,*: p≤0.05, **: p≤0.001, ***: p≤0.0001, as determined by one-way ANOVA, followed by Tukey's multiple comparisons test"
NCBI gene IDs:
"CHGA: 1113
KRT20: 54474
LGR5: 8549
MUC2: 4583"
Figure legend: "ACE2 mRNA expression in organoids grown in FM or in basic medium and either untreated or treated with IFN-γ (n=3). Data are presented as mean +/- SD ,*: p≤0.05, **: p≤0.001, ***: p≤0.0001, as determined by one-way ANOVA, followed by Tukey's multiple comparisons test"
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "B) qPCR data displaying the relative virus load of SARS-CoV-2 measured by viral genome quantity in full medium vs. basic medium organoids 48 h after infection and normalized to GAPDH (n=6). C) qPCR data displaying the relative virus load of SARS-CoV-2 measured by viral genome quantity in full medium vs. full medium + IFN-γ treated organoids 48 h after infection and normalized to GAPDH (n=9). D) qPCR data displaying the relative virus load of SARS-CoV-2 measured by viral genome quantity in basic medium vs. basic medium + IFN-γ treated organoids 48 h after infection and normalized to GAPDH (n=4). E) qPCR data displaying the relative virus load of SARS-CoV-2 measured by viral genome quantity in full medium + IFN-γ vs. basic medium+ IFN-γ treated organoids 48 h after infection and normalized to GAPDH (n=4). (B-E) Data are presented as mean +/- SD ,*: p≤0.05, **: p≤0.001, ***: p≤0.0001, as determined by Student's t-test "
NCBI gene IDs:
"GAPDH: "
Figure legend: "F) qPCR data comparing the relative virus load of SARS-CoV-2 measured by viral genome quantity normalized to GAPDH at 24 and 48 h after infection in full medium (left) and basic medium (right) untreated or treated with IFN-γ, indicating increase in virus load in differentiated and IFN-γ treated conditions (n=8). Data are presented as mean +/- SD ,*: p≤0.05, **: p≤0.001, ***: p≤0.0001, as determined by one-way ANOVA, followed by Tukey's multiple comparisons test"
NCBI gene IDs:
"GAPDH: "
Figure legend: "D) Relative expression of selected enterocyte-specific genes upon SARS-CoV-2 infection, normalized to GAPDH. Differences in gene expression were assessed using the linear model 'lmFit' and 'makeContrasts' in limma."
NCBI gene IDs:
"GAPDH: "
Figure legend: "F) qPCR data displaying the increase of INF-γ target genes (CXCL11, IFIT3) (upper) and enterocyte marker genes (KRT20, ACE2) (lower) after SARS-CoV-2 infection, validating the results from the microarray analysis (n=3). Data are presented as mean +/- SD ,****: p≤0.0001, as determined by Student's t-test"
NCBI gene IDs:
"ACE2: 59272
CXCL11: 6373
IFIT3: 3437
KRT20: 54474"
Figure legend: "(c) Midguts from control Atg18KG03090/wild-type (Atg18/+), n = 14, and Atg18KG03090/Df(3L)6112 mutant (Atg18/Df), n = 11, animals at puparium formation analysed by differential interference contrast microscopy. Representative images are shown. (d) Wild-type, control (Atg18KG03090/+) and Atg18 mutant (Atg18/Df) midgut cell size quantification (μm2) at indicated stages; n = 10 animal intestines per genotype with 5 cells measured per intestine per stage."
NCBI gene IDs:
"Atg18: 38913"
Figure legend: "(e) Differential interference contrast images of midgut cells from Atg2EP3697/wild-type control (Atg2/+) and Atg2EP3697/Df(3L)6091 mutant (Atg2/Df) animals at puparium formation. Representative images are shown. (f) Cell size quantification (μm2) from e, n = 12 (Atg2/+) and n = 7 (Atg2/Df) animal intestines per genotype with 5 cells measured per intestine."
NCBI gene IDs:
"Atg2: 38344"
Figure legend: "(g-j) Representative TEM images of intestine cells 2 h after puparium formation. (g,i) Control Atg18KG03090/wild-type (Atg18/+, g) and control Atg2EP3697/wild-type (Atg2/+, i) cells with an enlarged image showing a double-membrane structure (arrowhead) that surrounds a mitochondrion and endoplasmic reticulum. Arrows indicate autolysosomes. (h,j) Atg18KG03090/Df(3L)6112mutant (Atg18/Df, h) and Atg2EP3697/Df (Atg2/Df, j) mutant cells lacking autophagic structures. Quantification is shown as mean ± s.d. Scale bars, 20 μm (a-c and e) and 1 μm (g-j)."
NCBI gene IDs:
"Atg18: 38913
Atg2: 38344"
Figure legend: "(a) Midguts dissected from animals expressing Atg18IR specifically in DsRed-marked clones of cells at puparium formation and analysed by fluorescence and differential interference contrast (DIC) microscopy. Representative images are shown. (b) Quantification (μm2) from a, n = 12 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Atg18: 38913"
Figure legend: "(c) Midguts expressing mCherry-Atg8a in all cells, and expressing Atg18IR specifically in GFP-marked cell clones dissected from animals at 2 h after puparium formation. Representative images are shown."
NCBI gene IDs:
"Atg18: 38913"
Figure legend: "(d) Midguts dissected from animals at puparium formation that contain an Atg1Δ3D loss-of-function mutant cell clone (lacking GFP) and analysed by fluorescence and differential interference contrast microscopy. Wild-type (+/+) control cell possesses stronger GFP and heterozygous Atg1Δ3D/wild-type (Δ3D/+) cells have weaker GFP. Representative images are shown. (e) Quantification (μm2) from d, n = 7 (+/+), n = 8 (Δ3D/+) and n = 8 (Δ3D/Δ3D) animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Atg1: 39454"
Figure legend: "(f) Midguts dissected from animals expressing mCherry-Atg8a in all cells, and expressing Atg1IR specifically in GFP-marked clones of cells at 2 h after puparium formation. Representative images are shown."
NCBI gene IDs:
"Atg1: 39454"
Figure legend: "(g-j) Representative cut views from three-dimensional reconstitution images from wild-type (g,h) and Atg1 knockdown (i,j) cells at early third instar larval stage (g,i) and at puparium formation (h,j). (k) Quantification of cell volume (μm3) from g-j, n = 5 animal intestines per genotype with 1-5 cells measured per intestine per stage. Black columns: early third instar larval stage. White columns: puparium formation stage. Quantification is shown as mean ± s.d. NS, not significant. Scale bars, 20 μm."
NCBI gene IDs:
"Atg1: 39454"
Figure legend: "(a) Midguts dissected from animals at puparium formation that contain Vps34Δm22 loss-of-function mutant cell clones (lacking GFP) and analysed by fluorescence and differential interference contrast (DIC) microscopy. Wild-type (+/+) control cells possess stronger GFP and heterozygous Vps34Δm22/wild-type (Δm22/+) cells have weaker GFP. Representative images are shown. (b) Quantification (μm2) from a, n = 14 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Vps34: 37733"
Figure legend: "(c) Midguts dissected from animals expressing Atg8aIR specifically in GFP-marked clones of cells at puparium formation and analysed by fluorescence and differential interference contrast microscopy. Representative images are shown. (d) Cell size quantification (μm2) from c, n = 10 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Atg8a: 32001"
Figure legend: "(e) Midguts dissected from animals expressing Atg12IR specifically in DsRed-marked clones of cells at puparium formation and analysed by fluorescence and differential interference contrast microscopy. Representative images are shown. (f) Cell size quantification (μm2) from e, n = 14 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Atg12: 39383"
Figure legend: "(g-i) Midguts dissected from early third instar larvae that mis-express Atg1GS10797 (Atg1; g, GFP in nucleus and cytoplasm), both Atg1 +Atg8aIR (h), or both Atg1 +Atg7IR (i) and stained to detect Discs large (green) on the cortex of all cells. Representative images are shown. (g' and i') DIC images of midguts expressing mCherry-Atg8a in all cells, Atg1 (g'), and Atg1+Atg7IR (i') in GFP-marked clones of cells. mCherry-Atg8a puncta are shown in insets. Representative images are shown. Quantification is shown as mean ± s.d. Scale bars, 20 μm."
NCBI gene IDs:
"Atg1: 39454
Atg7: 37141
Atg8a: 32001"
Figure legend: "(a) Midguts dissected from animals at puparium formation that contain Atg7d4 loss-of-function mutant cell clones (lacking GFP) and analysed by fluorescence and differential interference contrast (DIC) microscopy. Wild-type (+/+) control cells possess stronger GFP and heterozygous Atg7d4/wild-type (d4/+) cells have weaker GFP. Representative images are shown. (b) Cell size quantification (μm2) from a, n = 8 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Atg7: 37141"
Figure legend: "(c) Midguts expressing mCherry-Atg8a in all cells, and with Atg7d4 loss-of-function clone cells (lacking GFP) at 2 h after puparium formation. Representative images are shown."
NCBI gene IDs:
"Atg7: 37141"
Figure legend: "(f) Midguts expressing GFP-Atg5 in enterocytes (larger nuclei) with an Atg7d4 loss-of-function clone cell (lacking RFP) at puparium formation. Representative images are shown."
NCBI gene IDs:
"Atg7: 37141"
Figure legend: "(g,h) Representative TEM images of intestine cells 2 h after puparium formation. Arrows indicate autolysosomes. (g,h) Control (Atg7d30/d14; g) and Atg7 mutant (Atg7d77/d14; h) cells both contain autophagic structures. (h, right) Enlarged Atg7 mutant cell image of a double-membrane autophagosome (arrowhead) surrounding a mitochondrion. Quantification is shown as mean ± s.d. NS, not significant. Scale bars, 20 μm (a,c,d and f) and 1 μm (g,h)."
NCBI gene IDs:
"Atg7: 37141"
Figure legend: "(a) Quantification of mitochondria numbers from Atg18 control (Atg18KG03090/wild type) and mutant (Atg18KG03090/Df(3L)6112) or Atg7 control (Atg7d30/d14) and mutant (Atg7d77/d14) TEM images. Mitochondria were quantified from 2 distinct 25 μm2 regions per cell from 2 cells per animal from at least 3 different animals per genotype."
NCBI gene IDs:
"Atg18: 38913
Atg7: 37141"
Figure legend: "(b,c) Midguts dissected from either early third instar larvae (b) or at puparium formation (c) that express GFP-labelled mitochondria in all cells and contain an Atg1Δ3D loss-of-function mutant cell clone (lacking RFP). Wild-type control cells possess stronger RFP and heterozygous cells have weaker RFP. Representative images are shown. Quantification is shown as mean ± s.d. NS, not significant. Scale bars, 0 μm."
NCBI gene IDs:
"Atg1: 39454"
Figure legend: "(a) Midguts dissected from animals expressing Uba1IR specifically in GFP-marked clones of cells at puparium formation and analysed by fluorescence and differential interference contrast (DIC) microscopy. Representative images are shown. (b) Cell size quantification (μm2) from a; n = 16 animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Uba1: 35998"
Figure legend: "(c) Midguts dissected from animals at puparium formation that contain Uba1H33 loss-of-function mutant cell clones (lacking RFP) and analysed by fluorescence and differential interference contrast microscopy. Wild-type (+/+) control cells possess stronger RFP and heterozygous Uba1H33/wild-type (H33/+) cells have weaker RFP. Representative images are shown. (d) Quantification (μm2) from c, n = 8 (+/+), n = 11 (H33/+) and n = 11 (H33/H33) animal intestines per genotype with 1-5 cells measured per intestine."
NCBI gene IDs:
"Uba1: 35998"
Figure legend: "(e) Midguts dissected from animals at puparium formation that contain Uba1H33 loss-of-function MARCM mutant cell clones (GFP-positive) that also have mCherry-Atg8a expressed in all cells and analysed by fluorescence microscopy. Control wild-type and heterozygous cells have no GFP. Representative images are shown."
NCBI gene IDs:
"Uba1: 35998"
Figure legend: "(f) Midguts expressing GFP-Atg5 in enterocytes (larger nuclei), and with an Uba1H33 loss-of-function clone cell (lacking RFP) at puparium formation analysed by fluorescence microscopy. Representative images are shown."
NCBI gene IDs:
"Uba1: 35998"
Figure legend: "(g) Midguts dissected from animals at puparium formation that contain Uba1H33 loss-of-function MARCM mutant cell clones (GFP-positive) that are stained with p62 antibody and analysed by fluorescence microscopy. Control wild-type and heterozygous cells have no GFP. Representative images are shown."
NCBI gene IDs:
"Uba1: 35998"