EMBO/SourceData huggingface dataset:
Figure legend: "Representative kinetics (K) and analysis (L) of aequorin-based [Ca2+]m measurements in intact MICU1-KO cells generated using the CRISPR/Cas9 technique, transfected with the indicated constructs and challenged with 20 μM TBHQ in the absence of extracellular Ca2+ (n=3 independent experiments.)"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Representative kinetics (M) and analysis (N) of aequorin-based [Ca2+]m measurements in intact MICU1-KO cells generated using the CRISPR/Cas9 technique, transfected with the indicated constructs and challenged with 10 μM CPA in the presence of 100 μM EGTA (n=3 independent experiments)."
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Hek293T cells were transfected with the GFP-tagged wild-type (WT) MICU1 and then treated with 1 μM rapamycin (Rapa.) alone for 4 h or in combination with the Akt inhibitor triciribine (10 μM). GFP-MICU1 immunocomplexes were precipitated with a GFP antibody and analyzed with PAS (phospho-Akt substrate) and phosphorylated (S473) Akt (p-Akt) antibodies by western blotting"
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Hek293T cells were transfected with either the GFP-tagged wild-type (WT) MICU1 or MICU1 S124A-GFP mutant. MICU1-GFP immunocomplexes were precipitated with a GFP antibody and analyzed with PAS (phospho-Akt substrate) antibody by western blotting."
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Mitochondrial preparations of both Hek293T (left) and HeLa (right) cells transiently transfected with an HA-tagged constitutively active Akt T308D/S473D (Akt D/D) construct were analyzed by western blotting. GAPDH: cytosolic marker; TIM23: mitochondrial marker"
NCBI gene IDs:
"Akt: 11651"
Figure legend: "Resting mitochondrial calcium levels in the control (ctrl) and Akt D/D-expressing HeLa cells evaluated by ratiometric imaging of the mitochondrial-targeted GCaMP6m (n=4 independent experiments; 44-49 cells)"
NCBI gene IDs:
"Akt: 11651"
Figure legend: "Schematic model of the HA-tagged constitutively active Akt form targeted to the mitochondrial intermembrane space (mt-Akt D/D). Both the expression and the mitochondrial localization of the chimera were assessed by immunofluorescence. TOM20 was used as a mitochondrial marker. Merged images are indicated (merge). Scale bar 10 μm"
NCBI gene IDs:
"Akt: 11651"
Figure legend: "Resting mitochondrial calcium levels in control (ctrl) and mitochondrial-targeted Akt D/D (mt-Akt D/D)-expressing HeLa cells, evaluated through ratiometric imaging of the mitochondrial-targeted GCaMP6m (n=4 independent experiments; 43-47 cells)"
NCBI gene IDs:
"Akt: 11651"
Figure legend: "Resting mitochondrial calcium levels in ShRNA MICU1 HeLa stable cells transfected with the indicated constructs and evaluated through ratiometric imaging of the mitochondrial targeted GCaMP6m (n=3 independent experiments; 38-50 cells)"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Representative kinetics (H) and analysis (I) of aequorin-based [Ca2+]m measurements in permeabilized ShRNA MICU1 HeLa stable cells transfected with the indicated constructs and challenged with 400 nM buffered [Ca2+] (n=3 independent experiments)."
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Western blot analysis of ShRNA MICU1 HeLa stable cells transfected with the indicated constructs. pre-MICU1: precursor form of MICU1; m-MICU1: mature form of MICU1"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Western blot analysis of ShRNA MICU1 HeLa stable cells transfected with the indicated constructs. Where indicated, the cells were treated with 5 μM MG132 for 4 h. pre: precursor; int: intermediate; m: mature form"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Western blot analysis of the supernatant (S) and insoluble pellet (P) fractions of ShRNA MICU1 HeLa cell mitochondria transfected with the indicated constructs, following carbonate extraction at pH 10. The established integral membrane protein TIM23 and the soluble protein cytochrome c (Cyt. c) were analyzed as markers. The cells were treated with 5 μM MG132 for 4 h before fractionation"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Western blot analysis of the supernatant (S) and insoluble pellet (P) fractions of ShRNA MICU1 HeLa cell mitochondria transfected with MICU1 WT-HA or MICU1 SD-HA, together with MCU-flag and EMRE constructs, following carbonate extraction at pH 7.4, pH 10.5, pH 11.5 or pH 12.5. The cells were treated with 5 μM MG132 for 4 h before fractionation"
NCBI gene IDs:
"MCU: 215999
MICU1: 10367
MICU1: 216001
EMRE: 69029"
Figure legend: "ShRNA MICU1 HeLa stable cells were transfected with the indicated constructs. Cells were incubated with 50 μg/ml cycloheximide (CHX) for the indicated times, collected and subjected to western blotting analysis as indicated. In the graph, the amount of HA (MICU1), both WT and mutants, is represented relative to the amount at time 0. (n=3 independent experiments)"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "ShRNA MICU1 HeLa stable cells were transfected with the indicated constructs. Cells were harvested, and total protein was extracted and subjected to western blotting analysis with the indicated antibodies. Immunoblotting was performed under reducing (with the presence of DTT) or non-reducing (non-red) conditions (without DTT). Cells were treated with 5 μM MG132 for 4 h before lysis"
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Resting mitochondrial calcium levels, evaluated through ratiometric imaging of the mitochondrial targeted GCaMP6m, in ShRNA MICU1 HeLa stable clone cells transfected with the indicated constructs (n=3 independent experiments; 46-54 cells)."
NCBI gene IDs:
"MICU1: 10367"
Figure legend: "Western blot analysis of endogenous MICU1 levels in HeLa cells upon silencing of IMMP1L and/or IMMP2L. int: intermediate; m: mature form"
NCBI gene IDs:
"IMMP1L: 196294
IMMP2L: 83943"
Figure legend: "Western blot analysis of HeLa cells transfected with the indicated constructs and silenced with IMMP1L and/or IMMP2L. int: intermediate; m: mature form. Cells were treated with 5 μM MG132 for 4 h before lysis"
NCBI gene IDs:
"IMMP1L: 196294
IMMP2L: 83943"
Figure legend: "Western blot analysis of the supernatant (S) and insoluble pellet (P) fractions of control or IMMP1L-silenced HeLa cell mitochondria following carbonate extraction at pH 10. The established integral membrane protein TIM23 and the soluble protein cytochrome c (Cyt. c) were analyzed as markers"
NCBI gene IDs:
"IMMP1L: 196294"
Figure legend: "Western blot analysis of the supernatant (S) and insoluble pellet (P) fractions of control or IMMP1L-silenced HeLa cell mitochondria transfected with HA-tagged MICU1 WT following carbonate extraction at pH 10. The established integral membrane protein TIM23 and the soluble protein cytochrome c (Cyt. c) were analyzed as markers. pre: precursor; int: intermediate; m: mature form. Cells were treated with 5 μM MG132 for 4 h before lysis"
NCBI gene IDs:
"IMMP1L: 196294
MICU1: 216001"
Figure legend: "Control or IMMP1L-silenced HeLa cells were transfected with HA-tagged MICU1 WT. Cells were incubated with 50 μg/ml cycloheximide (CHX) for the indicated times, collected and subjected to western blotting analysis as indicated. In the graph, the amount of HA (MICU1) is represented relative to the amount at time 0. (n=3 independent experiments)"
NCBI gene IDs:
"IMMP1L: 196294
MICU1: 216001"
Figure legend: "Representative kinetics (F) and analysis (G) of aequorin-based [Ca2+]m measurements in control or IMMP1L-silenced HeLa cells challenged with 10 μM cyclopiazonic acid (CPA) in the presence of 100 μM EGTA (n=3 independent experiments)"
NCBI gene IDs:
"IMMP1L: 196294"
Figure legend: "Resting mitochondrial calcium levels, evaluated through ratiometric imaging of the mitochondrial targeted GCaMP6m, in control or IMMP1L-silenced HeLa cells (n=3 independent experiments; 37-55 cells)."
NCBI gene IDs:
"IMMP1L: 196294"
Figure legend: "Lung cancer cells were transfected with the GFP-tagged WT MICU1. MICU1-GFP immunocomplexes were precipitated with a GFP antibody and analyzed with a PAS (phospho-Akt substrate) antibody by western blotting"
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Melanoma cells were transfected with the GFP-tagged WT MICU1. MICU1-GFP immunocomplexes were precipitated with a GFP antibody and analyzed with PAS (phospho-Akt substrate) antibody by western blotting"
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Glioblastoma cells were transfected with the GFP-tagged WT MICU1. MICU1-GFP immunocomplexes were precipitated with GFP antibody and analyzed with PAS (phospho-Akt substrate) antibody by western blotting"
NCBI gene IDs:
"MICU1: 216001"
Figure legend: "Representative images (J) and analysis (K) of MitoSOX-based ROS measurements in ShRNA MICU1 HeLa stable cells stably expressing either MICU1 S124D (SD) or MICU1 S124A (SA) (n=3 independent experiments). Scale bar 10 μm. f.a.u.: fluorescence arbitrary unit"
NCBI gene IDs:
"MICU1: 10367
MICU1: 216001"
Figure legend: "ShRNA MICU1 HeLa stable cells stably expressing either MICU1 S124D (SD) or MICU1 S124A (SA) were treated with 500 μM H2O2 alone or in combination with the ROS scavenger Tempol and analyzed by western blotting as indicated. l. e.: long exposure."
NCBI gene IDs:
"MICU1: 10367
MICU1: 216001"
Figure legend: "(g) Fold change in the  expression  of HBE1 or HBG1-2  transcripts ± SEM normalized to  KLF1 in CFUs obtained from HE  cells treated with UK5099 (10 µM) or  DCA (3 mM) relative to non-treated cells (n=3 biological replicates, paired t-test). Data information: ns, not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001"
NCBI gene IDs:
"HBE1: 3046
HBG1: 3047
KLF1: 10661"
Figure legend: "(d)  HE  cells were transduced with shScrambled (shScr) or shLSD1 with or without UK5099 (10 µM) the day after the sort  and day 3  CD43+/GPA+ cell frequencies ± SEM relative to shScr are presented (n=3 biological replicates 1-2 technical replicates each, one-way ANOVA test). Data information: ns, not significant, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001"
NCBI gene IDs:
"LSD1: 23028"
Figure legend: "(n) Dot plots showing gene expression  levels of NOTCH1, JAG1 and HES1 detected by  scRNAseq and based on percent expressed (size of the dots) and average level of  expression (color intensity)."
NCBI gene IDs:
"HES1: 3280
JAG1: 182
NOTCH1: 4851"
Figure legend: "A. Evaluation of siRNA-mediated depletion. U2OS cells were treated with control, Plk4 or hSAS-6 siRNA and immunoblotting was performed with the indicated antibodies. Asterisk indicates non-specific bands. The positions of molecular weight markers (kDa) are shown on the right."
NCBI gene IDs:
"Plk4: 10733
SAS-6: 163786"
Figure legend: "C, D, E. Quantification of the dispersion of satellite components upon depletion of Plk4 but not hSAS-6 knockdown. The results of PCM1 (C), hMsd1/SSX2IP (D) or BBS4 (E) are shown. Data represent the mean + SD (&gt;200 cells, n=3). Statistical analysis was performed using two-tailed unpaired student's t-tests. **p&lt;0.01, n.s. (not significant)."
NCBI gene IDs:
"Plk4: 10733
SAS-6: 163786"
Figure legend: "F, G. U2OS cells were arrested in G1 and treated with control or Plk4 siRNA, followed by immunofluorescence microscopy by using indicated antibodies. Enlarged images (marked by arrowheads) are shown on the right. Quantification of PCM1 distribution is shown in G. &gt;200 cells were counted and classified into two categories: normal (around and away from the centrosome) or dispersed. Data represent the mean + SD (n=3). Statistical analysis was performed using two-tailed unpaired student's t-tests. **p&lt;0.01. Scale bar, 1 μm (F, right), 10 μm (F)."
NCBI gene IDs:
"Plk4: 10733"
Figure legend: "H, I. siRNA-treated U2OS cells were transfected with empty vectors (EV) or plasmids containing Plk4 or siRNA-resistant Plk4-myc WT* or KD* and immunostained with the indicated antibodies. Cell peripheries are marked with solid lines, in which cells with yellow lines are successfully transfected (positive in myc signals), while those with white lines represent non-transfected cells. Quantification of PCM1 distribution is shown in I. Data represent the mean + SD (&gt;200 cells, n=3). Statistical analysis was performed using two-tailed unpaired student's t-tests. ****p&lt;0.0001, **p&lt;0.01, n.s. (not significant). Scale bar, 5 μm (H)."
NCBI gene IDs:
"Plk4: 10733"
Figure legend: "A, B. Either empty vector plasmids (EV) or those producing EGFP-PCM1 (A) or myc-Plk4 (B) were transfected into U2OS cells. Immunoprecipitation (IP) was performed with anti-GFP (A) or anti-myc antibodies (B), followed by immunoblotting with the indicated antibodies. DIC, the dynein intermediate chain."
NCBI gene IDs:
"PCM1: 5108
Plk4: 10733"
Figure legend: "C. Bacterially expressed and purified His-PCM1 (a.a. 1-1128) was incubated with MBP alone or MBP-Plk4 (WT or KD) in vitro, and pulled down by magnetic beads coupled with an anti-MBP antibody, followed by staining with Coomassie Blue (left) or immunoblotting with an anti-His antibody (right). Asterisks show non-specific bands. The mobility difference between Plk4-WT and Plk4-KD was reported previously [43, 48]. We reproducibly observed the upward mobility shift of His-PCM1 upon binding to MBP-Plk4-WT, but not Plk4-KD."
NCBI gene IDs:
"Plk4: 10733"
Figure legend: "E. Total cell extracts were prepared from U2OS cells treated with PCM1 siRNA and transfected with RNAi-resistant EGFP-PCM1-WT*. Cells were cultured asynchronously (As) or arrested in G1, G2 or M phase, followed by immunoprecipitation with an anti-GFP antibodies. Pulled down samples were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "A, B, C, D. siRNA-treated U2OS cells (PCM1 for A and B; Plk4 for C and D) were transfected with plasmids producing various EGFP-PCM1 constructs (WT*, S372A*, S372D* or S372E*), fixed and stained with the indicated antibodies (arrowheads point to the centrosome) (A and C). Quantification data are shown in B and D. &gt;200 cells were counted and classified into three categories: normal (around and away from the centrosome), aggregated or dispersed. Data represent the mean + SD (n=3). Statistical analysis was performed using two-tailed unpaired student's t-tests. ***p&lt;0.001, **p&lt;0.01, *p&lt;0.05, n.s. (not significant). Note that the percentage of cells displaying \"dispersed\" is analysed. Scale bars, 5 μm (A and C)."
NCBI gene IDs:
"PCM1: 5108
Plk4: 10733"
Figure legend: "E. PCM1 siRNA-treated U2OS cells were transfected with two types of plasmids (EGFP- and mCherry-tagged) that produce PCM1-WT* and PCM1-S372A*. Immunoprecipitation and subsequent immunoblotting were performed with the indicated antibodies. Quantification data is shown on the right. Statistical analysis was performed using two-tailed unpaired student's t-tests. *p&lt;0.05, **p&lt;0.01, n.s. not significant."
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "F. U2OS cells were treated with PCM1 siRNA and transfected with empty vectors (EV) or EGFP plasmids that produce PCM1-WT* or PCM1-S372A*. Immunoprecipitation was performed with an anti-GFP antibody, followed by immunoblotting with the indicated antibodies."
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "A. Immunofluorescence microscopy was performed using the indicated antibodies in hTERT-RPE-1 cells treated with control, PCM1 or Plk4 siRNA. Regions around the basal body (insets) are enlarged on the right hand side. Scale bars, 5 μm (left) and 1 μm (right).B. Quantification. At least 150 cells were counted (n=3). Statistical analysis was performed using two-tailed unpaired student's t-tests. ***p&lt;0.001, ****p&lt;0.0001."
NCBI gene IDs:
"PCM1: 5108
Plk4: 10733"
Figure legend: "C. hTERT-RPE-1 cells treated with PCM1 siRNA were transfected by an empty vector (EV) or plasmids producing PCM1-WT*, -S327A* or -S372E*, and immunofluorescence microscopy performed. Enlarged images of the regions around the basal body are shown in insets. Scale bars, 5 μm and 1 μm (inset)."
NCBI gene IDs:
"PCM1: 5108"
Figure legend: "B. Representative 2D projection of confocal  images showing caspase  activation at the dissection  time point  (red channel) and in the recent past (green channel, arrows) within putative escort somatic cells via DBS-S-QF  sensor; TUNEL staining  indicates apoptosis  (grey, arrowhead); DAPI  labels the nuclei  in the entire Figure. Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection. Genotype: Actin DBS-S-QF, UAS-mCD8-GFP, QUAS-tomato-HA/+; ;QUAS-Gal4  /+ (BL83123). C. Representative 2D projection of confocal  images showing escort  and follicular somatic cells permanently labelled with DBS-S-QF sensor (green channel, arrows); the arrowhead indicates the presence of  germline cells  positive for TUNEL staining  (red, arrowhead). Notice the lack of TUNEL  signal and therefore apoptosis  in somatic cells  permanently labelled with  DBS-S-QF sensor (green). Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection. Genotype: Actin DBS-S-QF, UAS-mCD8-GFP, QUAS-tomato-HA/+; QUAS-flippase  (BL30126)/+; Actin5C  FRT-stop-FRT lacZ-nls/+ (BL6355) Data information: Scale bars represents 10 µm in the entire Figure."
NCBI gene IDs:
"Actin: 
Actin5C: 
flippase: 
GFP: 
HA: 
lacZ: 
tomato: 
CD8: 12525
Gal4: 855828"
Figure legend: "D. Graph showing the percentage of germaria  permanently labelled  with DBS-S-QF overtime at 25 °C (N=4 biological replicates; n=520 and n=536 germaria  were inspected at 7 and 14 days post adult eclosion, respectively) and 29 °C (N=7, n=799 at 7 days and n=588 at 14 days). A two-way ANOVA and Tukey's multiple comparison tests were used to determine statistical significance (n.s = not significant; **** p&lt;0.0001). Genotype: Actin DBS-S-QF, UAS-mCD8-GFP, QUAS-tomato-HA/+; QUAS-flippase  (BL30126)/+; Actin5C  FRT-stop-FRT lacZ-nls/+ (BL6355). Data information: The box plots show the median, first quartile, and third quartile of datasets. The whiskers illustrate the range between the maximum and minimum values of datasets."
NCBI gene IDs:
"Actin: 
Actin5C: 
flippase: 
GFP: 
HA: 
lacZ: 
tomato: 
CD8: 12525"
Figure legend: "A-B. Representative 2D projection of confocal  images showing the expression  of the cell identity Castor  in follicular cells  control with Dronc expression  (A: 109-30-Gal4  (BL7023)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/+) and without Dronc  (B: 109-30-Gal4  (BL7023)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/ UAS-flippase (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF). Notice the reduction in the number of Castor-expressing cells in the FCD  and the reduction in size of this region in B. Nuclei  are labelled with DAPI  (blue); Castor  (green) and FasIII  (grey) label the follicular cells; UAS-Histone-RFP  labels the nuclei  of Gal4-expressing cells (red). Data information: Scale bars represents 10 µm. Experimental flies  were kept after eclosion from the pupae for 14 days at 29°C prior to dissection (applicable to all panels)."
NCBI gene IDs:
"flippase: 
GFP: 
Histone: 
RFP: 
Dronc: 39173
Gal4: 855828
Tub: 40554"
Figure legend: "C. Quantification of total number of follicular cells  (left) and Castor-expressing cells (right) within the FCD  (FasIII  positive region) in either heterozygous or homozygous Dronc  mutant conditions: Genotypes: CTRL= 109-30-Gal4  (BL7023)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/+; Dronc -/- = 109-30-Gal4  (BL7023)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/ UAS-flippase  (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF.CTRL=ptc-Gal4  (BL2017)/+; UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/+. Dronc  -/- = ptc-Gal4  (BL2017)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/ UAS-flippase  (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-QF. N=2; the n number for each column in order of appearance n=16, n=14, n=20, n=17, n=19, n=18. Statistical significance was determined by using an unpaired parametric Welch's t-test (****p≤0.001). The median and quartiles are indicated in the violin plots. Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels)."
NCBI gene IDs:
"flippase: 
GFP: 
Histone: 
RFP: 
Dronc: 39173
Gal4: 855828
Tub: 40554"
Figure legend: "E. Percentage of germaria  showing TUNEL staining  in different cell types of the germarium  (somatic cells  and germline cells) upon altering Dronc  expression in all of the  escort cells  and follicular stem cells. N=3 biological replicates; the n number of inspected germaria  per condition is indicated close to the genotype. The genotypes in the graph are: White triangle (n=378 germaria) = ptc-Gal4  (BL2017)/+; +/+. Yellow circle (n=381) = ptc-Gal4  (BL2017)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/ +. Red square (n=196) = ptc-Gal4  (BL2017)/+; DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/ UAS-flippase  (BL8209) DroncKO-FRT-Dronc-GFP-APEX-FRT-suntag-HA. Blue circle (n=184)= ptc-Gal4  (BL2017)/UAS-Dronc  (BL56198); DroncKO UAS-Histone-RFP  (BL56555) Tub-G80ts (BL7019)/+. An ordinary one-way ANOVA and Holm-Sidak's multiple comparison test was used to determine statistical significance (n.s = not significant; ** p≤0.01; *** p≤0.001; ****p≤0.0001). The graph shows the mean and standard deviations. Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels)."
NCBI gene IDs:
"flippase: 
GFP: 
HA: 
Histone: 
RFP: 
suntag: 
Dronc: 39173
Gal4: 855828
Tub: 40554"
Figure legend: "A. Quantification of total number of follicular cells in germaria  with  follicular stem cells expressing normal levels of Dronc  (DroncKO/+), without Dronc  expression (DroncKO/DroncKO- Suntag-HA-Cherry), and expressing a catalytically inactive form of Dronc  (DroncKO/DroncKO- FL-CAEA-Suntag-HA-Cherry ). The genotypes are as follows: 109-30-Gal4  (BL7023)/+ (n=13). 109-30-Gal4  (BL7023)/+; DroncKO Tub-G80ts (BL7019) / UAS-flippase  (BL8209) DroncKO-FRT  Dronc-GFP-Apex  FRT-Suntag-HA-Cherry    (n=15). 109-30-Gal4  (BL7023)/+;  DroncKO Tub-G80ts (BL7019) / UAS-flippase  (BL8209) DroncKO-FRT Dronc-GFP-Apex  FRT-Dronc  FL-CAEA-Suntag-HA-Cherry  (n=14). A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (***p≤0.001; ****p≤0.0001). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria  containing one single group of germline cells wrapped by follicular cells."
NCBI gene IDs:
"Cherry: 
flippase: 
GFP: 
HA: 
Suntag: 
Dronc: 39173
Gal4: 855828
Tub: 40554"
Figure legend: "D. Quantification of total number of  follicular cells  within the FasIII  cellular domain in the following genotypes: 109-30-Gal4/+; Tub-G80ts (BL7019)/+ (n=16). 109-30-Gal4  (BL7023)/UAS-DriceRNAi UAS-DecayRNAi (a gift from Pascal Meier); UAS-DammRNAi, UAS-Dcp1RNAi (a gift from Pascal Meier) (n=15). 109-30-Gal4  (BL7023)/+; Tub-G80ts (BL7019)/ UAS-Dcp1RNAi (BL28909) (n=25) 109-30-Gal4  (BL7023)/+; Tub-G80ts (BL7019)/ UAS-DriceRNAi (BL32403) (n=16) 109-30-Gal4  (BL7023)/UAS-Dark-sh; Tub-G80ts (BL7019)/+ (a gift from M. Miura) (n=18). 109-30-Gal4  (BL7023)/UAS-microRNA-RHG; Tub-G80ts (BL7019)/+ (a gift from Iswar Hariharan) (n=10). A one-way ANOVA and Dunnett's multiple comparison post-test was used to determine statistical significance (***p≤0.001; ****p≤0.0001). Data information: Experimental flies  were kept after eclosion from the pupae  for 14 days at 29°C prior to dissection (applicable to all panels). All the experimental data shown have been obtained from N ≥ 2 biological replicates. The median and quartiles are indicated in the violin plots. All the quantifications were made in germaria containing one single group of  germline cells wrapped by follicular cells."
NCBI gene IDs:
"Damm: 36266
Dark: 36914
Dcp1: 37790
Decay: 42008
Drice: 43514
Gal4: 855828
RHG: 37038
Tub: 40554"