EMBO/SourceData huggingface dataset:
Figure legend: "Representative gene loci (TLR2 (top) and S100A7A/8/9/12 (bottom) H3K27ac peaks) comparing SC-naïve (blue) and SC-conv (red) monocytes. Red peaks reach higher values than blue peaks (representative examples of four samples for each condition)."
NCBI gene IDs:
"S100A7A: 338324
TLR2: 7097"
Figure legend: "(B)  YTS KIR2DL1 cells were transfected with either N.S siRNA (blue curve) or with SHP-1  and Cbls siRNA (red curve). After 48 hours, cells were loaded with  calcium-sensitive Fluo-3-AM and analyzed for basal intracellular calcium levels for 1 min. The NK cells were then mixed with 721.221 Cw4 target cells at 37°C and monitoring of calcium levels was continued. The data shown are representative of three independent experiments."
NCBI gene IDs:
"Cbls: 868///23624
Cw4: 3107
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(C)  YTS KIR2DL1 cells were incubated with either 721.221 Cw4 or Cw7 target cells for 2 hours and analyzed by flow cytometry to determine the expression of CD107a. Expression of  CD107a  was measured by mean fluorescence Intensity (MFI). Relative expression of CD107a  MFI was normalized to the mock-transfected sample following  Cw4 incubation. Data are means ± SEM of five independent experiments.(n=5). P values were calculated by One-sample t-tests and One way ANOVA following Tukey's post hoc analysis are indicated by asterisks *P &lt; 0.05, ****P&lt;0.0001 ."
NCBI gene IDs:
"Cw4: 3107
Cw7: 3107
KIR2DL1: 3802"
Figure legend: "(E) Killing assay. YTS KIR2DL1 cells were mock-transfected or transfected with N.S siRNA or SHP-1 and Cbls siRNA. After 48 hours, cells were incubated with  [35S] Met-loaded 721.221 Cw4  or  Cw7 target cells. After 5 hours of co-culture, the supernatant was collected, and the radioactive  signal was measured. Data are means ± SEM of three independent experiments. P values were calculated by One-way ANOVA following Tukey's post hoc analysis and are indicated by asterisks *P &lt; 0.05, **P &lt; 0.01 ."
NCBI gene IDs:
"Cbls: 868///23624
Cw4: 3107
Cw7: 3107
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(A) NK92-NKp46low, NK92-NKp46high, YTS KIR2DL1,  K562 and  721.221 HLA-Cw4 cells were  stained with anti-NKp46  monoclonal antibody followed by staining  with Alexa568-Fluor goat anti-mouse IgG secondary antibody. Cells were then analyzed using flow cytometry. (B) NK92-NKp46low, NK92-NKp46+, YTS KIR2DL1, K562, and 721.221 HLA-Cw4 cells were incubated with 50 µg rhodamine-labeled NPs and analyzed using  flow cytometry to confirm the internalization of the coated NPs in a NKp46-specific manner. "
NCBI gene IDs:
"HLA-Cw4: 3107
KIR2DL1: 3802
NKp46: 9437"
Figure legend: "(C-F) (C) NK92-NKp46low, (D) NK92-NKp46high, (E) YTS KIR2DL1 and (F) K562  cells were incubated with 50 µg rhodamine-labeled NPs and analyzed using confocal microscopy. Upper panel shows wide field images. Lower panel shows single-cell images. The data shown are representative of three independent experiments."
NCBI gene IDs:
"KIR2DL1: 3802
NKp46: 9437"
Figure legend: "(A) YTS KIR2DL1 and  primary NK cells were incubated with NPs loaded with  Cbl-b, c-Cbl  and SHP-1 siRNA, or NPs loaded with 1500 pmol N.S siRNA. Following 48 hours of incubation, cells were lysed, and the membrane was  blotted with anti-Cbl-b, anti-c-Cbl or anti-SHP-1 antibodies. GAPDH served as loading control. Densitometric analysis of the bands was performed using ImageJ and normalized to the GAPDH densitometry values. Analysis by ImageJ densitometry  is summarized in the graph below. Data are means ± SEM of four independent experiments. (n=4). P values were calculated by One-sample t-tests and are indicated by asterisks. *P &lt; 0.05, **P &lt; 0.01."
NCBI gene IDs:
"Cbl-b: 868
c-Cbl: 23624
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(B) YTS KIR2DL1 cells were incubated with SHP-1 and Cbls siRNAs-loaded NPs (red curve), with NS siRNA loaded NPs (green curve) or with empty NPs  (blue curve). After 48 hours, cells were loaded with calcium-sensitive Fluo-3-AM and analyzed for basal intracellular calcium levels for 1 min. The NK  cells were then mixed with 721.221 Cw4  target cells at 37°C, and monitoring of calcium levels was continued. The data shown are representative of three independent experiments."
NCBI gene IDs:
"Cbls: 868///23624
Cw4: 3107
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(D)  YTS KIR2DL1 cells were treated with SHP-1 and Cbls  siRNAs-loaded NPs  or N.S siRNA-loaded  NPs. After 48 hours, cells were incubated with either  721.221 Cw4  or Cw7 target cells, the supernatant was then collected, and granzyme B levels were evaluated using ELISA sandwich  assay. The levels of granzyme B were quantified based on a standard curve. Data are means ± SEM of five independent experiments. (n=5). P values were calculated by One way ANOVA following Tukey's post hoc analysis, and are indicated by asterisks. **P &lt; 0.01."
NCBI gene IDs:
"Cbls: 868///23624
Cw4: 3107
Cw7: 3107
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(F) pNK cells were pretreated with either SHP-1 and Cbls siRNA-loaded NPs or N.S siRNA-loaded  NPs. After 48 hours, cells were incubated with either 721.221 Cw4 or Cw7 target cells for 2 hours and analyzed by flow cytometry to determine the expression of CD107a. Expression of CD107a was quantitated by mean fluorescence intensity (MFI). The data represent five independent experiments (n=5) Data are shown as mean ± SEM.) (n=5). P values were calculated by One-sample t-tests and One way ANOVA following Tukey's post hoc analysis, and are indicated by asterisks. *P≤0.05."
NCBI gene IDs:
"Cbls: 868///23624
Cw4: 3107
Cw7: 3107
SHP-1: 5777"
Figure legend: "(A)  YTS KIR2DL1 cells were incubated with SHP-1  and Cbls  siRNA-loaded NPs (red curve) or empty NPs (purple curve). After 48 hours, cells were loaded with  Fluo-3-AM and analyzed for intracellular calcium  levels for 20 min at 37°C.  YTS KIR2DL1 cells that were treated with PBS  served as a negative control (green curve). NK cells that were mixed with 721.221 Cw7  target cells served as a positive control (blue curve). Calcium levels were analyzed by spectrofluorometry. (n=3)"
NCBI gene IDs:
"Cbls: 868///23624
Cw7: 3107
KIR2DL1: 3802
SHP-1: 5777"
Figure legend: "(B) Freshly isolated PBMCs were treated with SHP-1  and Cbls siRNA-loaded NPs, empty NPs, PBS as negative control or PHA as a positive control. Following incubation, supernatant from each sample was collected and the levels of  human  cytokines  TNF-α, IL-6, IL-10 and IFN-γ were determined using ELISA. The data represent four independent experiments (n=4). P values were calculated by One way ANOVA following Tukey's post hoc analysis, and are indicated by asterisks. *P≤0.008. Data are shown as mean ± SEM."
NCBI gene IDs:
"Cbls: 868///23624
SHP-1: 5777"
Figure legend: "(E) Following 24 hours after the last treatment, extracted cells from tumors of either SHP-1 and Cbls or N.S siRNA loaded NKp46-NPs treated mice  were analyzed by flow cytometry.  Expression of CD107a was measured by mean fluorescence Intensity (MFI). Relative expression of CD107a MFI was normalized to the sample treated with N.S siRNA loaded  NPs. The data represent three independent experiments. Data are shown as mean ± SEMn=3; P value was calculated by one sample t-test and is indicated within the graph P =0.009."
NCBI gene IDs:
"Cbls: 868///23624
SHP-1: 5777"
Figure legend: "(F)  Survival  analysis in mice treated with NK cells' gene silenced for SHP-1  and Cbls using siRNA-loaded  NPs  (n=18) or N.S siRNA-loaded NPs (n=18) as demonstrated by Kaplan-Meier survival curve. P values were calculated using the log-rank test and is indicated within the graph P =0.00009."
NCBI gene IDs:
"Cbls: 868///23624
SHP-1: 5777"
Figure legend: "d, STX17 knockdown COS7 cells expressing GFP-ATG14 and RFP-SEC61b; were immunostained for TOMM20. Error bars denote s.d."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "e, Immuoblot of subcellular fractions of STX17 knockdown (using two STX17 siRNAs, STX17-1 and STX17-2) HEK293 cells expressing GFP-DFCP1."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "b, Electron micrographs of STX17 knockdown cells. White arrows denote autolysosomes, black arrows denote autophagosome, white arrowheads mark isolation membranes."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "c, Immunogold electron micrographs of STX17 knockdown HeLa cells stained for GFP-LC3."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "d, GFP-ATG5 dots (arrows) in STX17 knockdown HeLa cells. e, Quantification of staining in d."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "f, Immunoblot of p62 in STX17 knockdown cells. Tubulin was used as a loading control."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "g, Degradation of long-lived proteins in STX17 knockdown cells. St, starved; wor, wortmannin. All error bars denote s.d. Scale bars, 1 µm (a, b), 200 nm (c) and 5 µm (d)."
NCBI gene IDs:
"STX17: 55014"
Figure legend: "C. Average dynamics of nucleoid separation and cell constriction for WT and the ΔmatP mutant strain. The cumulative distributions of the fraction of cells with two nucleoids (blue) and of the fraction of cells with a constriction degree above 0.15 (red) were plotted against cell age. Cell age was calculated according to the rank of each cell based on their cell length with the formula $\text{age}_{i}\left( F \right) = \ - \frac{ln(1 - \frac{F}{2})}{ln(2)}$, where F represents the fraction of cells with a cell length equal or below the length of cell i (Wold et al, 1994). D. Same plots as in C for three strains clustering in island 8 with the ΔmatP strain. The WT curves shown in C were plotted in gray for comparison. E. Same plots as in C for two island-8 strains, ΔycjV and ΔhslU, and the ΔhslV strain, which does not partition in island 8. "
NCBI gene IDs:
"hslU: 948430
hslV: 948429
matP: 945570
ycjV: 945890"
Figure legend: "B. The cumulative distribution of the proportion of constricting cells for the ftsA* mutant and its parent were plotted against cell age. We measured the degree of constriction of all cells using Oufti. For each strain, we had two independently acquired image sets (n = 496 and 1,168 cells for the WT replicates and n = 692 and 1,506 cells for the ftsA* replicates). The distributions of the degree of constriction were not significantly different among the four datasets at a threshold p-value of 0.01 using a Kruskal-Wallis multi-comparison test. Moreover, Bonferroni-corrected post-hoc pairwise tests did not allow a distinction between WT and ftsA* samples."
NCBI gene IDs:
"ftsA: 944778"
Figure legend: "C. Scatter plot of the CV of the cell length for the whole population versus the CV of the cell length for constricting cells only. The contour lines represent the 0.5, 075 and 0.95 probability envelopes of the 240 independent WT replicates. The gray color levels indicate the density of points in the vicinity of each strain. The ∆mraZ strain discussed in the text is highlighted in red."
NCBI gene IDs:
"mraZ: 944810"
Figure legend: "(a) Differential interference contrast micrographs of root epidermal cells incubated for 8 h with concanamycin A, in nutrient starvation conditions. Autophagic bodies accumulation in the vacuole is clearly visible in wild-type seedlings, absent in the atg5-1 mutant and restored in atg5-1 seedlings expressing ATG5-GFP. (b) Confocal microscopy. The ATG5-GFP fluorescence pattern in the atg5-1 background is identical to that observed in wild-type seedling, including ring-like structures. Scale bar, 15 μm."
NCBI gene IDs:
"atg5: 831594"
Figure legend: "(A) Phenotypes of wild type (Col), kup9 mutants (kup9-1 and kup9-2) and the kup9-1/ProKUP9:KUP9 complementation lines (COM1 and COM2). Seeds were germinated and grown on low-K+ (LK, 50 μM) or high-K+ (HK, 5 mM) medium for 7 d. Scale bar, 1 cm."
NCBI gene IDs:
"kup9: 827740
KUP9: 827740"
Figure legend: "(D) RT-qPCR analysis of KUP9 transcript levels in various materials. Data are means ± SE (n = 3, biological replicates; each replicate contains 120 individual plants)."
NCBI gene IDs:
"KUP9: 827740"
Figure legend: "(A) Root meristem zones of wild type (Col), kup9 mutants (kup9-1 and kup9-2) and the kup9-1/ProKUP9:KUP9 complementation lines (COM1 and COM2). Seeds were germinated and grown on LK or HK medium for 7 d. The meristem zone lengths are marked with red lines. Scale bars, 50 μm."
NCBI gene IDs:
"kup9: 827740
KUP9: 827740"
Figure legend: "(A) to (F) Expression of KUP9 as determined in the ProKUP9:GUS line. GUS staining of 5-day-old seedling (A), root maturation zone (B), transverse section of maturation zone (C) and (D), primary root tip (E), and root apex (F). Ep, epidermis; Co, cortex; En, endodermis; Pe, pericyle; Xy, xylem; Ph, phloem. Scale bars, 5 mm in (A), 1 mm in (B), 10 μm in (C), 5 μm in (D), 1 mm in (E), and 20 μm in (F)."
NCBI gene IDs:
"GUS: 
KUP9: 827740"
Figure legend: "(G) and (H) Expression of KUP9 protein as determined in the kup9-1/ProKUP9:KUP9-GFP transgenic plants. Green fluorescence shows the subcellular localization of KUP9-GFP. PI (propidium iodide) and ER tracker were used to indicate the positions of the plasma membrane (PM) and endoplasmic reticulum (ER) and are shown as red and cyan fluorescence, respectively. Scale bars for root tip, 10 μm in (G) and 5 μm in (H). Scale bars for EZ and MAZ, 20 μm."
NCBI gene IDs:
"GFP: 
kup9: 827740
KUP9: 827740"
Figure legend: "(A) QC cell observation in wild type and the kup9 mutants using the QC marker lines ProWOX5:GFP and ProQC25:GUS. Seeds were germinated on HK medium for 4 d, then the seedlings were transferred to LK or HK medium for the indicated times. Scale bars, 10 μm."
NCBI gene IDs:
"GUS: 
QC25: 
kup9: 827740
WOX5: 820297"
Figure legend: "(B) QC division rates of wild type and the kup9 mutants after LK treatment for 96 h. The statistical method is described in Materials and Methods. Data are means ± SE (n = 150, individual plants). Student's t-test (**P &lt; 0.01) was used to analyze statistical significance, and &quot;#&quot; represents control."
NCBI gene IDs:
"kup9: 827740"
Figure legend: "(A) Auxin levels in root tips, as indicated by the ProDR5:GFP reporter line. Seeds were germinated on HK medium for 4 d, then the seedlings were transferred to LK or HK medium for the indicated times. Scale bar, 10 μm. (B) Quantification of GFP fluorescence in the QC cells shown in (A). Data are means ± SE (n = 20, individual plants). Student's t-test (**P &lt; 0.01) was used to analyze statistical significance. "
NCBI gene IDs:
"DR5: 
GFP: "
Figure legend: "(C) QC cell observation using the QC marker line ProWOX5:GFP. Seeds were germinated on HK medium for 4 d, then the seedlings were transferred to LK or HK medium with or without NAA (100 nM) for 96 h. Scale bar, 10 μm."
NCBI gene IDs:
"GFP: 
WOX5: 820297"
Figure legend: "Phenotypes of the kup9-1/ProWOX5:AMI1 (A) transgenic lines. Seeds were germinated and grown on LK or HK medium with or without IAM for 7 d. Scale bars, 1 cm."
NCBI gene IDs:
"AMI1: 837418
kup9: 827740
WOX5: 820297"
Figure legend: "Phenotypes of the kup9-1/ProKUP9:AMI1 (C) transgenic lines. Seeds were germinated and grown on LK or HK medium with or without IAM for 7 d. Scale bars, 1 cm."
NCBI gene IDs:
"AMI1: 837418
kup9: 827740
KUP9: 827740"
Figure legend: "(B) 3H-IAA accumulation in the BY-2 cells expressing the indicated proteins. PIN4-GFP and KUP10-GFP were used as positive control and negative control, respectively. Data are means ± SE (n = 3, biological replicates; each replicate contains 3-4 mL suspension cells). Student's t-test (*P &lt; 0.05, **P &lt; 0.01) was used to analyze statistical significance, and &quot;#&quot; represent control."
NCBI gene IDs:
"GFP: 
KUP10: 839997
PIN4: 814670"
Figure legend: "(D) 3H-IAA accumulation in the oocytes expressing KUP9-GFP and PIN4 at the indicated times. PIN4 was used as a positive control. The oocytes were incubated in the bath solution containing 3H-IAA for the indicated times. Data are means ± SE (n = 6, biological replicates; each replicate contains 6 oocytes)."
NCBI gene IDs:
"GFP: 
KUP9: 827740
PIN4: 814670"
Figure legend: "(F) K+ efflux activity of the oocytes expressing KUP9 and NRT1.5. The oocytes were not injected with IAA. The oocytes were incubated in K+-free bath solution for 6 h. Data are means ± SE (n = 3, biological replicates; each replicate contains 6 oocytes)."
NCBI gene IDs:
"KUP9: 827740
NRT1.5: 840139"
Figure legend: "(C) to (E) The contents of free IAA (C), IA-Asp (D), and IA-Glu (E) in the root tips of wild type and the kup9-1 mutant. Data are means ± SE (n = 3, biological replicates; each replicate contains 1000-1200 individual plants). Student's t-test (*P &lt; 0.05, **P &lt; 0.01) was used to analyze statistical significance, and &quot;#&quot; represents control."
NCBI gene IDs:
"kup9: 827740"
Figure legend: "(F) Phenotype of the kup9-1/ProKUP9:PIN8 transgenic lines. Seeds were germinated and grown on LK or HK medium for 7 d. Scale bar, 1 cm."
NCBI gene IDs:
"kup9: 827740
KUP9: 827740
PIN8: 831362"
Figure legend: "(B) Interaction between PLD1 and ARL11/14 is stronger than with ARF1/6, RhoA. Flag-tagged PLD1 was co-expressed with HA-tagged GTP-bound mutants of small GTPases in HEK293A cells as indicated. PLD1 was immunoprecipitated with Flag beads, and the co-precipitated small GTPases were detected with an HA antibody. The result is a representative of three biologically independent experiments."
NCBI gene IDs:
"Flag: 
GTP: 
HA: 
PLD1: 5337"
Figure legend: "(C) ARL11/14 co-localize with PLD1. Flag-tagged PLD1 was co-expressed with HA-tagged GTP-bound mutants of small GTPases in HEK293A cells as indicated. After 24 h, cells were fixed with 4% paraformaldehyde, followed by incubation with anti-Flag and anti-HA primary antibodies. The Flag and HA-antibodies were detected by anti-mouse and anti-rabbit secondary antibodies, respectively. DAPI (blue) was used for DNA staining. Scale bars: 10 μm. The result is a representative of two biologically independent experiments."
NCBI gene IDs:
"GTP: 
HA: "
Figure legend: "(E) PLD1 preferentially binds to the GTP-form, not the GDP-form, of ARL11. Flag-tagged PLD1 was co-expressed with HA-tagged ARL11Q67L (GTP-form) or ARL11T26N (GDP-form) mutant in HEK293A cells. The result is a representative of two biologically independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
ARL11: 115761
PLD1: 5337"
Figure legend: "(F) The subcellular co-localization of ARL11 with PLD1 is GTP-dependent. HA-tagged GTP-bound ARL11Q67L, wild-type ARL11 or GDP-bound ARL11T26N was co-expressed with Flag-tagged PLD1. After 24 h, cells were fixed, followed by incubation with anti-Flag and anti-HA primary antibodies. DAPI (blue) was used for DNA staining. Scale bars: 10 μm. The result is a representative of three biologically independent experiments."
NCBI gene IDs:
"Flag: 
ARL11: 115761
PLD1: 5337"
Figure legend: "(B) The loop region of PLD1 is required for the interaction between ARL11 and PLD1. Flag-tagged full-length or truncations of PLD1 was co-expressed with HA-tagged ARL11Q67L, PLD1 was immunoprecipitated with Flag beads, and ARL11 that associated with it was detected with an HA antibody. The result is a representative of two biologically independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
ARL11: 115761
PLD1: 5337"
Figure legend: "(C) Deletion of the loop region disrupts the PLD1 and ARL11 co-localization. Flag-tagged full-length or ∆Loop truncation of PLD1 was co-expressed with HA-tagged ARL11Q67L. After 24 h, cells were fixed with 4% paraformaldehyde, followed by incubation with anti-Flag and anti-HA primary antibodies. DAPI (blue) was used for DNA staining. Scale bars: 10 μm. The result is a representative of three biologically independent experiments. (D) Mander's overlap coefficient of (J). Mander's overlap coefficient calculated using JACoP plugin in ImageJ."
NCBI gene IDs:
"Flag: 
HA: 
ARL11: 115761
PLD1: 5337"
Figure legend: "(B) ARL11 Knockout of THP-1 stable cell pools. ARL11 knockout THP-1 cell pools were generated by the CRISPR/Cas9 technology with three different guide RNAs. Cell lysates were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"CRISPR: 
ARL11: 115761
Cas9: 69900935"
Figure legend: "(D) THP-1 cell pool stably expressing HA-tagged ARL11Q67L or ARL11T26N. Lysates were immunoblotted with indicated antibodies."
NCBI gene IDs:
"HA: 
ARL11: 115761"
Figure legend: "(E) ARL11Q67L, but not ARL11T26N, promotes phagocytosis of macrophages. Macrophages were differentiated from indicated THP1 cell pool, followed by incubated with green zymosan particles for 1 hour. Scale bars: 50 μm. The result is a representative of two biologically independent experiments."
NCBI gene IDs:
"ARL11: 115761"
Figure legend: "(G) PLD1 knockout efficiency and HA-tagged ARL11Q67L expression of THP-1 stable cell pools. Lysates were immunoblotted with indicated antibodies."
NCBI gene IDs:
"HA: 
ARL11: 115761
PLD1: 5337"
Figure legend: "(H) PLD1 knockout blocks the ARL11Q67L-stimulated phagocytosis. Macrophages were differentiated from indicated THP1 cell groups and then incubated with green zymosan particles for 1.5 hours. Scale bars: 50 μm. The result is a representative of two biologically independent experiments. (I) PLD1 is required for ARL11 to stimulate phagocytosis. Data are quantification of panel (H)."
NCBI gene IDs:
"ARL11: 115761
PLD1: 5337"
Figure legend: "(B) Interaction of PI4KB with ARL5A/5B is stronger than ARF1, RAB11A/11B, and RAB14. Flag-tagged PI4KB was co-expressed with HA-tagged GTP-bound mutant of GTPases in HEK293A cells as indicated. PI4KB was immunoprecipitated with Flag beads, and the co-precipitated GTPases were detected by HA Western."
NCBI gene IDs:
"Flag: 
HA: 
PI4KB: 5298"
Figure legend: "(C) PI4KB preferentially binds to the GTP-form of ARL5B. Flag-tagged PI4KB was co-expressed with HA-tagged ARL5BQ70L (GTP-form) or ARL5BT30N (GDP-form) mutant in HEK293A cells. Interaction was measured similar to panel B. The result is a representative of two biologically independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
ARL5B: 221079
PI4KB: 5298"
Figure legend: "(D) The ARL5A colocalization with PI4KB is GTP-dependent. HA-tagged GTP-bound ARL5AQ70L, wild-type ARL5A or GDP-bound ARL5AT30N was co-expressed with Flag-tagged PI4KB. After 24 h, cells were fixed, followed by incubation with anti-Flag and anti-HA primary antibodies. DAPI (blue) was used for DNA staining. Scale bars: 10 μm. Shown is a representative of two biologically independent experiments. The left panel is the quantification of overlap coefficient. Mean± SD; n= 6 technically independent samples."
NCBI gene IDs:
"HA: 
ARL5A: 26225"
Figure legend: "(E) ARL5A/5B double knockout diminishes the colocalization of PI4KB and TGN46. Flag-tagged PI4KB was expressed in HEK293A wild-type or two ARL5A/5B double knockout clones as indicated. After 24 hours, cells were fixed, followed by incubation with anti-Flag and anti-TGN46 primary antibodies. DAPI (blue) was used for DNA staining. Scale bars: 5 μm. The result is a representative of two biologically independent experiments. The left panel is the quantification of overlap coefficient."
NCBI gene IDs:
"ARL5A: 26225"
Figure legend: "(G) The N1 region of PI4KB is required for the interaction with ARL5B. Flag-tagged full-length or truncations of PI4KB were co-expressed with HA-tagged ARL5BQ70L. PI4KB was immunoprecipitated with Flag beads, and the co-precipitated ARL5B was detected by HA Western. The result is a representative of three biologically independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
ARL5B: 221079
PI4KB: 5298"
Figure legend: "(H) The N1 region of PI4KB is required for the co-localization of ARL5A/B and PI4P. P4Mx2-mEGFP was co-expressed with HA-tagged ARL5AQ70L in HEK293A wild-type or PI4KB knockout cells (clone#2-5). HEK293A cells were co-transfected with indicated constructs. After 24 h, cells were fixed with 4% paraformaldehyde, followed by incubation with anti-HA primary antibodies. DAPI (blue) was used for nuclei staining. Scale bars: 10 μm. The result is a representative of two biologically independent experiments. (I) Mander's overlap coefficient of (H)."
NCBI gene IDs:
"HA: 
ARL5A: 26225
PI4KB: 5298"