EMBO/SourceData huggingface dataset:
Figure legend: "(O) quantification of the ratio of the dorsal surface peak of ATP5 intensity to the second highest peak for the genotypes indicated. ***, p&lt;0.001; **p&lt;0.01; *p&lt;0.05; ns, not significant by Mann-Whitney test. n=9 (btz/+), n=11 (Df/+, btz/Df), n=6 (btz/Df; G14&gt;btz, C57-GAL4/+), n=4 (btz/Df; Btz-WT, C57&gt;eIF4AIII RNAi), n=5 (btz/Df; Btz-HD), n=10 (C57&gt;btz RNAi), or n=3 (C57&gt;mago RNAi). Error bars show mean ± SEM."
NCBI gene IDs:
"HD: 
btz: 43331
Btz: 43331
eIF4AIII: 40987
Df: 248315
GAL4: 855828"
Figure legend: "(A-J) confocal images of the NMJ on larval muscles 6 and 7 in segment A3, stained with anti-HRP (magenta) to label the nerve and anti-Dlg (green) as a postsynaptic marker of synaptic boutons. (A) w1118 control; (B) btz2/+; (C) btz2/Df(3R)BSC497; (D) UAS-btz/+; da-GAL4, btz2/Df(3R)BSC497; (E) BG380-GAL4/+; UAS-btz/+; btz2/Df(3R)BSC497; (F) 24B-GAL4/UAS-btz; btz2/Df(3R)BSC497; (G) BG380-GAL4/+; 24B-GAL4/UAS-btz; btz2/Df(3R)BSC497; (H) BG380-GAL4/+; UAS-btz RNAi/+; (I) mhc-GAL4/UAS-btz RNAi; (J) repo-GAL4/UAS-btz RNAi. Scale bar, 30 μm. Insets in (A, C) show enlargements of the boxed regions, illustrating the closer spacing of boutons in btz mutants."
NCBI gene IDs:
"btz: 43331
da: 34413
Df: 248315
GAL4: 855828
mhc: 35007
repo: 47285"
Figure legend: "(K, L) quantifications of the number of boutons normalized to muscle surface area (x1000) in the indicated genotypes. ****, p&lt;0.0001; ***, p&lt;0.001; **, p&lt;0.01; ns, not significant by unpaired t-test with Welch's correction. n=23 (w; da-GAL4, btz/Df), n=6 (btz/+), n=10 (Df/+, UAS-BtzGFP/+; btz/Df), n=8 (btz/Df), n=22 (da&gt;btz-GFP; btz/Df), n=13 (BG380-GAL4; btz/Df; BG380+24B&gt;Btz-GFP; btz/Df; da-GAL4/+), n=5 (BG380&gt;Btz-GFP; btz/Df), n=11 (24B-GAL4; btz/Df; da&gt;btz RNAi), n=12 (24B&gt;Btz-GFP; btz/Df; btz RNAi/+), n=9 (BG380+24B-GAL4; btz/Df), n=15 (BG380-GAL4/+), n=14 (BG380&gt;btz RNAi; repo&gt;btz RNAi), n=16 (mhc-GAL4/+), n=28 (mhc&gt;btz RNAi), or n=21 (repo-GAL4/+). Error bars show mean ± SEM. NMJ size is reduced in btz mutants and rescued by expressing UAS-btz with da-GAL4 or with the combination of the BG380-GAL4 and 24B-GAL4 drivers, but not with any single driver. Expressing UAS-btz RNAi with da-GAL4 or in motor neurons with BG380-GAL4, muscles with mhc-GAL4 or glia with repo-GAL4 also reduces NMJ size."
NCBI gene IDs:
"GFP: 
btz: 43331
Btz: 43331
da: 34413
Df: 248315
GAL4: 855828
mhc: 35007
repo: 47285"
Figure legend: "(I) quantifications of the number of boutons normalized to muscle surface area (x1000) in the indicated genotypes. ****, p&lt;0.0001; ***, p&lt;0.001 by unpaired t-test or Mann-Whitney test. n=15 (BG380-GAL4), n=12 (BG380&gt;btz RNAi; BG380&gt;mago RNAi; BG380&gt;eIF4AIII RNAi), n=16 (btz/+), n=13 (btz/Df), or n=10 (Btz-WT; btz/Df; Btz-HD; btz/Df). Error bars show mean ± SEM. NMJ size is reduced by knocking down btz in motor neurons, but slightly increased by knocking down mago or eIF4AIII. The size deficit in btz mutants is equally well rescued by the wild type or EJC interaction-defective btz transgenes."
NCBI gene IDs:
"HD: 
btz: 43331
Btz: 43331
eIF4AIII: 40987
Df: 248315
GAL4: 855828
mago: 37402"
Figure legend: "(L) quantifications of the number of boutons normalized to muscle surface area (x1000) in the indicated genotypes. ****, p&lt;0.0001; ns, not significant by unpaired t-test or Mann-Whitney test. n=19 (UAS-daw RNAi/+), n=9 (BG380&gt;daw RNAi; btz/Df; tub&gt;daw), n=23 (mhc&gt;daw RNAi), n=10 (repo&gt;daw RNAi; tub&gt;daw), n=12 (btz/Df; UAS-daw), or n=14 (btz/Df; tub&gt;btz). Error bars show mean ± SEM. Knocking down daw in motor neurons, muscles or glia reduces NMJ size. Expressing UAS-daw with tub-GAL4 rescues NMJ size in btz mutants as efficiently as expressing UAS-btz, but has no significant effect on NMJ size in a wild-type background."
NCBI gene IDs:
"btz: 43331
daw: 33474
Df: 248315
GAL4: 855828
mhc: 35007
repo: 47285
tub: 40554"
Figure legend: "mRNA expression  of Ifng, Tnfa, Il17f, and Il22. Colonic  transcription levels were normalized by Hprt, which was the most stable among the three housekeeping genes tested (Hprt, Hsp90, Gapdh). Longitudinal data of mean ± SEM is shown for 14SM FF, 14SM FR, and 13SM FR pups  at age   10-20 days (circles) with statistical significance calculated using two-way ANOVA factored on group and time point  compared to the 14SM FR  control. To the right of each longitudinal plot, we also report colonic  transcript levels for pups  born to germ-free (GF) FF  or FR   dams at age  15 days (squares) with statistical significance based on a one-way ANOVA with false-discovery adjustment using the Benjamini and Hochberg method. Outliers removed using ROUT method with Q = 10%. Data are from two independent experiments and represent biological replicates (n = 3-20/group)."
NCBI gene IDs:
"Gapdh: 14433
Hprt: 15452
Hsp90: 15516
Ifng: 15978
Il17f: 257630
Il22: 50929
Tnfa: 21926"
Figure legend: "A) We identified previously unknown telomere regulators in fission yeast using the haploid S. pombe whole-genome gene deletion library including ssu72+ (SPAC3G9.04). B) Telomere length of wt, ssu72Δ and ssu72-C13S (point mutation at the phosphatase active site) strains were measured by Southern blot in ApaI digested genomic DNA using a telomeric probe. "
NCBI gene IDs:
"ssu72: 2543452"
Figure legend: "C) Ssu72 recruitment to telomeres is cell cycle regulated. Ssu72 was myc-tagged at the 3' end and ChIP analysis was carried out in cell cycle synchronized populations of cdc25ts strains. Septa formation was used proxy for S-phase. n ≥ 3; *p ≤0.05 based on a two-tailed Student's t-test to ssu72+ control samples. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"myc: 
cdc25: 2542632
Ssu72: 2543452
ssu72: 2543452"
Figure legend: "D) Telomere length of ssu72Δ is dependent on telomerase. Diploid strains with the appropriate genotypes were sporulated and trt1∆ ssu72∆ double mutants were streaked for multiple passages (triangle indicates increased number of generations)."
NCBI gene IDs:
"ssu72: 2543452
trt1: 2540601"
Figure legend: "E) Telomerase is recruited to telomeres in the absence of Ssu72. ChIP analysis for Trt1-myc in wt and ssu72∆ was performed as described in material and methods using a non-tagged strain as a control. n ≥ 3; *p ≤0.05 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"Ssu72: 2543452
ssu72: 2543452"
Figure legend: "F) Telomerase activator Ccq1 is hyperphosphorylated in ssu72∆ cells. rap1∆ cells were used as positive control for Ccq1 phosphorylation status. Western blots were performed using Ccq1-flag tagged strains."
NCBI gene IDs:
"flag: 
Ccq1: 2539352
rap1: 2540115
ssu72: 2543452"
Figure legend: "A) ssu72Δ telomeres present longer G-rich overhangs than wt and rif1∆. In-gel hybridization in native and denaturing conditions was labelled with a radiolabelled C-rich telomere probe and quantified for ssDNA at the telomeres. n = 3; *p ≤0.05 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"rif1: 2542144
ssu72: 2543452"
Figure legend: "B) RPA (Rad11-GFP) is enriched at ssu72∆ telomeres. Colocalization of Rad11-GFP with Taz1-mCherry, used as a telomere marker was performed in wt and ssu72∆ cells; n =3; *p ≤0.05 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM). More than 1000 cells were analyzed for each genotype"
NCBI gene IDs:
"ssu72: 2543452"
Figure legend: "C) ssu72+ controls telomere length independently of rif1+. Epistasis analysis of telomere length of ssu72∆ and ssu72-C13S (catalytically inactive mutant) with rif1∆ was performed by Southern blotting of ApaI digested DNA using a telomeric probe. D) ssu72+ and stn1+ regulate telomere length in the same genetic pathway. Epistasis analysis of ssu72∆ and stn1-75 performed by Southern blotting of ApaI digested DNA using a telomeric probe. Two independently generated ssu72∆ stn1-75 double mutants are shown."
NCBI gene IDs:
"rif1: 2542144
ssu72: 2543452
stn1: 2541052"
Figure legend: "A) Ssu72 is required for telomere recruitment of Stn1 in late S phase. ChIP analysis of stn1-myc in wt and ssu72∆ cells was performed in synchronized cdc25ts cells. n ≥ 3; *p ≤0.05 based on a two-tailed Student's t-test to ssu72+ control samples. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"cdc25: 2542632
Ssu72: 2543452
ssu72: 2543452"
Figure legend: "B) 2D-gel analysis of NsiI telomeric fragments of wt and ssu72∆ strains. Smart ladder from Eurogentec was used for DNA size measurement."
NCBI gene IDs:
"ssu72: 2543452"
Figure legend: "C) Serine 74 substitution to a phosphomimetic aspartate amino acid (stn1-S74D) is sufficient to confer ssu72∆ telomere defects. Telomere length epistasis analysis of ssu72∆ and stn1-S74D mutants were performed by Southern blotting of ApaI digested genomic DNA using a telomeric probe."
NCBI gene IDs:
"ssu72: 2543452
stn1: 2541052"
Figure legend: "E) Similar to ssu72∆ mutants, stn1-S74D is defective in telomere recruitment. ChIP analysis of stn1-myc and stn1-S74D-myc using a non-tagged strain as a control. n = 3; *p ≤0.05 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"ssu72: 2543452
stn1: 2541052"
Figure legend: "A) ssu72+ and pol1+ regulate telomere length in the same genetic pathway. Epistasis analysis of ssu72∆ and pol1-13 mutants was performed by Southern blotting of ApaI digested DNA using a telomeric probe."
NCBI gene IDs:
"pol1: 2543629
ssu72: 2543452"
Figure legend: "B) Stn1-Pol1 interaction requires Ssu72 phosphatase. Immunoprecipitation experiments of Pol1-Flag with Stn1-Myc was preformed both in wt and ssu72∆ mutants. C) Immunoprecipitation of Ten1-Flag with Stn1-Myc in either wt or ssu72∆ was performed as control for Stn1-Ten1 complex integrity. "
NCBI gene IDs:
"Flag: 
Myc: 
Pol1: 2543629
ssu72: 2543452
Ssu72 phosphatase: 2543452
Stn1: 2541052
Ten1: 9406926"
Figure legend: "D) Overexpression of polymerase alpha partially rescues telomere length defect of ssu72Δ mutants. Multi-copy vector with polymerase α under thiamine promoter were expressed both in wt or ssu72Δ cells."
NCBI gene IDs:
"polymerase alpha: 2543629
polymerase α: 2543629
ssu72: 2543452"
Figure legend: "A) Telomere elongation of SSU72 down-regulated cells is telomerase dependent. Telomere Southern blot was carried out with HT1080 cells infected for 4 weeks with lentiviral particles carrying two independent shRNAs against SSU72 (CDS and UTR regions) and Luciferase (Luc) as control for shRNA. Knockdown efficiencies were determined by RT-qPCR using specific primers against hSSU72."
NCBI gene IDs:
"Luciferase: 
hSSU72: 29101
SSU72: 29101"
Figure legend: "B) Telomere elongation of SSU72 down-regulated cells is epistatic with STN1 downregulation. TRF analysis of HT1080 cells infected with the appropriate retroviral or lentiviral particles carrying either GFP shRNA, SSU72 shRNA (against UTR), STN1 shRNA or double SSU72 shRNA/STN1 shRNA. Cells were grown for 4 weeks and DNA was isolated to carry out Southern Blot analysis."
NCBI gene IDs:
"GFP: 
SSU72: 29101
STN1: 79991"
Figure legend: "C) hSSU72 down-regulation results in multi-telomeric signals (MTS) that are dependent on DNA replication. Visualization of mitotic spreads of HT1080 hSSU72 shRNA cells treated with aphidicolin and colcemid. Telomere FISH was carried out using a PNA-telomere probe. MTS are represented by arrows. Quantification of MTS per metaphase: n=4; **p ≤0.01 ****p ≤0.0001 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"hSSU72: 29101"
Figure legend: "A) hSSU72 downregulation results in telomere DNA damage foci (TIF). Cells with indicated shRNAs were fixed and IF-FISH analysis was performed using a 53BP1 antibody and a PNA-telomere probe. (Right) Quantification of cells with more than 5 telomeric 53BP1 foci: n=3; **p ≤0.01 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"hSSU72: 29101"
Figure legend: "B) hSSU72 is required for efficient loading of hSTN1 at human telomeres. ChIP was preformed using anti-FLAG antibodies and Southern blotting was performed using a human telomeric probe. Quantification of 3 independent ChIP experiments *p ≤0.05 **p ≤0.01 based on a two-tailed Student's t-test to control sample. Error bars represent standard error of the mean (SEM)."
NCBI gene IDs:
"hSSU72: 29101"
Figure legend: "C Representative D-type motor neurons in wild-type and mxl-1 mutant animals 24 h after laser surgery. In wild-type animals, a severed axon has regenerated a growth cone (arrow). In mutants, proximal ends of axons failed to regenerate (arrowhead). Scale bar = 10 μm."
NCBI gene IDs:
"mxl-1: 179557"
Figure legend: "B Induction of Psvh-2::nls::venus expression in D-type motor neurons by laser surgery. Expression of fluorescent proteins in D-type motor neurons of wild-type, mxl-1, tdpt-1 and mxl-1; tdpt-1 animals 3 h after laser surgery are shown. Yellow arrowheads and white arrows indicate axon and cell bodies, respectively, of D-type neurons. White arrows indicate the sites of laser surgery. D neurons are visualized by CFP under control of the unc-25 promoter. Cell bodies of D-type neurons are magnified and shown within the red boxes. Most of the intestinal fluorescence in these photos is from endogenous and variable background autofluorescence. A schematic representation of D-type motor neurons is shown on the bottom. Scale bars = 10 μm."
NCBI gene IDs:
"mxl-1: 179557
tdpt-1: 173276
unc-25: 176713"
Figure legend: "B Interaction of MXL-1 with TDPT-1 by yeast two-hybrid assays. The reporter yeast strain L40u was co-transformed with expression vectors encoding LexA DBD-MXL-1 and GAL4 AD-TDPT-1 as indicated. Yeasts carrying the indicated plasmids were grown on a selective plate lacking histidine and containing 10 mM 5-aminotriazole for 4 days."
NCBI gene IDs:
"GAL4: 855828
LexA: 20466968
MXL-1: 179557
TDPT-1: 173276"
Figure legend: "B Interaction between ETS-4 and TDPT-1. COS-7 cells were transfected with plasmids encoding T7-TDPT-1, HA-ETS-4 (WT), HA-ETS-4(S73E) (SE) and HA-ETS-4(S73A) (SA), as indicated. Whole-cell extracts (WCE) and immunoprecipitated complexes obtained with anti-T7 antibody (IP: T7) were analyzed by immunoblotting (IB)."
NCBI gene IDs:
"HA: 
T7: 
ETS-4: 180927
TDPT-1: 173276"
Figure legend: "C In vitro SUMOylation of ETS-4. COS-7 cells were transfected with plasmids encoding HA-ETS-4 (WT) and HA-ETS-4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti-HA antibody. The immunoprecipitates were subjected to in vitro SUMOylation assays. Reaction mixtures were analyzed by immunoblotting (IB) with anti-SUMO-2/3 and anti-HA antibodies. Arrows mark the SUMOylated-ETS-4 bands."
NCBI gene IDs:
"HA: 
ETS-4: 180927"
Figure legend: "E SUMOylation of ETS-4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA-ETS-4 (WT), HA-ETS-4(K32A; K83A) (2KA), T7-TDPT-1, His-SUMO-1, His-SUMO-2 and Myc-MXL-1, as indicated. SUMO-conjugated proteins were isolated by cobalt affinity chromatography. ETS-4 was detected by immunoblotting with anti-HA antibody. Whole-cell extracts (WCE) were analyzed by immunoblotting (IB)."
NCBI gene IDs:
"HA: 
His: 
Myc: 
SUMO-1: 
SUMO-2: 
T7: 
ETS-4: 180927
MXL-1: 179557
TDPT-1: 173276"
Figure legend: "A Effect of phosphorylation of ETS-4 on SUMOylation of ETS-4 in mammalian cells. HEK293 cells were transfected with plasmids encoding HA-ETS-4 (WT), HA-ETS-4(K32A; K83A) (2KA), HA-ETS-4(S73A) (SA), HA-ETS-4(S73E) (SE), T7-TDPT-1 and His-SUMO-2, as indicated. SUMO-conjugated proteins were isolated by cobalt affinity chromatography. ETS-4 was detected by immunoblotting with anti-HA antibody. Whole-cell extracts (WCE) were analyzed by immunoblotting (IB)."
NCBI gene IDs:
"HA: 
His: 
SUMO-2: 
T7: 
ETS-4: 180927
TDPT-1: 173276"
Figure legend: "B Effect of SUMOylation of ETS-4 on PKA phosphorylation of ETS-4 in vitro. COS-7 cells were transfected with plasmids encoding HA-ETS-4 (WT), HA-ETS-4(S73A) (SA) and HA-ETS-4(K32A; K83A) (2KA), as indicated. Cell lysates were immunoprecipitated with anti-HA antibody. The immunoprecipitates were subjected to in vitro PKA kinase assays or in vitro SUMOylation and then in vitro PKA kinase assays, using recombinant GST-fused active PKA, as indicated. Reaction mixtures were analyzed by immunoblotting (IB) with anti-phospho-PKA substrate, anti-SUMO-2/3 and anti-HA antibodies. Arrows mark the SUMOylated-ETS-4 bands. Asterisk indicates the non-SUMOylated- and phosphorylated-ETS-4 bands."
NCBI gene IDs:
"HA: 
ETS-4: 180927"
Figure legend: "B Expression levels of RBM15 and AS-RBM15 in different blood lineages by meta-analysis of RNA-seq data [38]: HSC (hematopoietic stem cells), MPP (multipotent progenitor cells), CMP (common myeloid progenitor cells), CLP (common lymphoid progenitor cells), GMP (Granulocyte-macrophage progenitor cells), MEP (megakaryocyte- erythrocyte progenitor cells), MK (megakaryocyte), and EB (erythroid body)."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "C Relative expression levels of AS-RBM15 (left) and RBM15 (right) in MEG-01 cells treated with PMA were measured by real-time PCR assays (n=4, mean ± SD). SD: standard deviation."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "D Relative expression levels of AS-RBM15 and RBM15 in CD34+ cells grown in pro-MK medium were measured by real-time PCR. GAPDH was used as an internal control (n=3, mean ± SD). Also see Fig EV 1."
NCBI gene IDs:
"AS-RBM15: 
GAPDH: 
RBM15: 64783"
Figure legend: "A CFU-MK assays with CD34+ cells expressing AS-RBM15. MK colonies staining for acetylcholinesterase activity were counted (right). P value was calculated using Student's t test (n=3, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "B The number of CD41+CD42+ mature MK cells was determined by FACS analysis. CD34+ cells expressing sh-AS-RBM15 were grown in pro-MK medium containing thrombopoietin and stem cell factor (TPO/SCF) for five days (n=4, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "D FACS analysis of CD34+ cells grown in pro-MK medium and expressing AS-RBM15 full length or Δ5 or Δ3. The CD41+CD42+ cell percentages were analyzed using one way ANOVA, ** P&lt;0.01, (n=4, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "E The percentage of CD41+CD42+ cells in CD34+ cells expressing AS-RBM15 Exon 1 were determined by FACS analysis after these cells were grown in pro-MK medium for five days. P value was calculated by t test, p&lt;0.01, (n=3, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "A RBM15 protein levels in leukemia cell lines (MEG-01, K562, HEL) were assessed by Western blotting (WB) (left) (n=3). The mRNA levels of RBM15 in cells overexpressing AS-RBM15 were measured by real-time PCR (right, n=3, mean ± SD). GAPDH was used as an internal control for WB and PCR."
NCBI gene IDs:
"AS-RBM15: 
GAPDH: 
RBM15: 64783"
Figure legend: "B Protein levels (left) and mRNA levels (right) of RBM15 in MEG-01 cells expressing two different shRNAs against AS-RBM15 respectively were measured using WBs and real-time PCR assays, respectively (n=3, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "C Protein levels (left) and mRNA levels (right) of RBM15 in MEG-01 cells expressing truncated AS-RBM15 RNAs (the schematic as shown on Fig 2C) were measured using WB and real-time PCR respectively (n=3, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "D The role of AS-RBM15 in protein translation was assessed by reporter assays. The reporter under control of the CMV promoter contains the 5'UTR of RBM15 mRNA ligated to firefly luciferase followed by an IRES element controlling Renilla luciferase as an internal control. The reporter plasmid was cotransfected with plasmids expressing full-length, exon 1 and Δ5 AS-RBM15 respectively. The vector reporter without 5'UTR is a negative control. (n=3, mean ± SD). P values were calculated by one-way ANOVA test (*p&lt;0.05, **p&lt;0.01 and ***p&lt;0.001)."
NCBI gene IDs:
"AS-RBM15: 
firefly: 
Renilla: 
RBM15: 64783"
Figure legend: "A Cytoplasmic extracts from MEG-01 cell lines stably expressing AS-RBM15, Exon 1, or shRNA-02 against AS-RBM15 were fractionated through sucrose gradients (15%-60%) (n=4). Global polyribosome profiles of these cell lines were generated by UV absorbance at 254 nm (A254). The arrow indicates increasing polyribosome weights. Low molecular weight, LMW; high molecular weight, HMW."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "B The distributions of RBM15 mRNA (top), GAPDH mRNA (middle), and AS-RBM15 (bottom) were measured by real-time PCR and represented as percentages of total RNA in the fractions (n=3)."
NCBI gene IDs:
"AS-RBM15: 
GAPDH: 2597
RBM15: 64783"
Figure legend: "C MS2 pull-down assays: AS-RBM15 full-length (FL) and exon 1 were fused to MS2RNA as baits. Plasmids expressing MS2 binding protein ( HA-tagged) and fusion baits were co-transfected into 293T cells. HA antibody was used to immunoprecipitateHA-MS2 binding protein as shown by a WB on the bottom panel. RBM15 mRNA and MS2 fusions were assessed by real-time PCR (n=3, mean ± SD). Fold enrichment was calculated as 2 to the power of [(CI.R-Cb.R)-(CI.MS2-Cb.MS2)]. I: input i.e. the RNA in solution before immunoprecipitation, R: RBM15 mRNA, b: bead-bound mRNA, MS2: RNA levels of MS2 fusions, C: number of cycles in real-time PCR. One-way ANONA for p value: p&lt;0.05."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "D WB analysis of the endogenous RBM15 protein levels in 293T cells overexpressing two different doses of AS-RBM15-MS2 and MS2 (n=3)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "E RBM15 mRNA interacts with AS-RBM15 via RNA-RNA interaction. AS-RBM15-MS2-associated RNAs were purified by phenol extraction and measured by reverse transcription and PCR for detection of RBM15, AS-RBM15 and MS2 under denaturing (heat) and non-denaturing conditions (n =3)."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "F RNase A protection assays for detecting endogenous association of AS-RBM15 and RBM15 mRNA (n=4). RNA was extracted from MEG-01 cells with phenol and digested with RNase A. cDNA was synthesized after RNase A treatment and detected using AS-RBM15 exon-specific primers as shown in the schematic diagram on top. RT: reverse transcriptase."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "G MEG-01 cells expressing AS-RBM15 or vector control were treated with DMSO or rapamycin for 45 minutes. Cells were harvested for WB using antibodies against GAPDH and RBM15 (n=2)."
NCBI gene IDs:
"AS-RBM15: "
Figure legend: "H The RBM15 5'UTR reporter remains sensitive to rapamycin treatment with or without overexpression of antisense AS-RBM15 (n=4, mean ± SD). P values were calculated by one-way ANOVA test (**p&lt;0.01 and ***p&lt;0.001)."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783"
Figure legend: "The subcellular distribution of RBM15 and AS-RBM15 in CD34+ cells grown in TPO-containing medium for 5 days. Cytoplasmic and nuclear RNA levels of AS-RBM15 and RBM15 were normalized to GAPDH (control for cytoplasmic fraction) and MALAT1 (control for nuclear fraction), respectively, using the ΔΔCt method (n=4, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: 
GAPDH: 
MALAT1: 
RBM15: 64783"
Figure legend: "A RUNX1 expression levels in human CD34+ cells grown in pro-MK medium in a time course were measured by real-time PCR (n=4, mean ± SD)."
NCBI gene IDs:
"RUNX1: 861"
Figure legend: "C The mRNA expression levels of RUNX1, RBM15, and AS-RBM15 in RUNX1 knockdown MEG-01 cells were measured by real-time PCR (n=4, mean ± SD)."
NCBI gene IDs:
"AS-RBM15: 
RBM15: 64783
RUNX1: 861"
Figure legend: "D Chromatin immunoprecipitation using anti-RUNX1 antibody. Immunoprecipitated DNA fragments were measured by real-time PCR using HEL cells expressing shRUNX1 and control (n=4, means ± SD)."
NCBI gene IDs:
"RUNX1: 861"