EMBO/SourceData huggingface dataset:
Figure legend: "(J-K) Confocal  images (J) and statistical analysis (K) of NPC1 patient fibroblasts  treated with 50 nM mock siRNA (siSCR) or siRNA targeting TPCN2  (siTPC2) for 72 hours. Cells were then treated with DMSO  or  TPC2-A1-P (30 µM). Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells); one-way ANOVA, Tukey's (K) multiple comparisons test. *p-value &lt; 0.05; **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"TPC2: 219931
TPCN2: 219931"
Figure legend: "Confocal  images (E-F) of MLIV patient fibroblasts  mock-electroporated and treated with DMSO  or electroporated with a gain-of-function hTPC2(M484L/G734E):mCherry  TOPO 3.1 vector (white arrow heads) and treated with either DMSO  or TPC2-A1-P (30 µM, 48h)."
NCBI gene IDs:
"mCherry: 
TPC2: 219931"
Figure legend: "statistical analysis (G) of MLIV patient fibroblasts  mock-electroporated and treated with DMSO  or electroporated with a gain-of-function hTPC2(M484L/G734E):mCherry  TOPO 3.1 vector (white arrow heads) and treated with either DMSO  or TPC2-A1-P  (30 µM, 48h). Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells); one-way ANOVA, post hoc Bonferroni's multiple comparisons test *p-value &lt; 0.05; **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"mCherry: 
TPC2: 219931"
Figure legend: "Confocal  images and statistical analysis of human  CTR patient fibroblasts  treated with 50 nM mock siRNA (siSCR) or siRNA targeting TPCN2  (siTPC2) for 72 hours. Cells were then treated with DMSO  Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells) two-tailed Student's t-test *p-value &lt; 0.05; **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"TPC2: 219931
TPCN2: 219931"
Figure legend: "Confocal  images and statistical analysis of human NPC1 patient fibroblasts  treated with 50 nM mock siRNA (siSCR) or siRNA targeting TPCN2  (siTPC2) for 72 hours. Cells were then treated with DMSO  or TPC2-A1-P (30 µM). Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells); two-tailed Student's t-test K). *p-value &lt; 0.05; **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"TPC2: 219931
TPCN2: 219931"
Figure legend: "Cortical neurons were differentiated from iPSCs, generating  lysosomal storage disease neurons  and isogenic controls. (C-D) Western blot  analysis of  cathepsin B (CtsB) in CTR and MCOLN1IVS3-2A&gt;G neurons  treated with TPC2-A1-P (30 µM) or DMSO. Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells, obtained from at least three distinct neuronal  differentiations); two-tailed Student's t-test (C). **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"MCOLN1: 57192"
Figure legend: "Cortical neurons  were differentiated from iPSCs, generating  lysosomal storage disease neurons  and isogenic controls. Cortical neurons were treated with compounds and acidic compartments stained  with LysoTracker (LyTr). Endolysosomal  expansion was observed in MCOLN1IVS3-2A&gt;G, CLN3D416G and CLN3ΔEx4-7 neurons, which was ameliorated by TPC2-A1-P  (30 µM) treatment. Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells, obtained from at least three distinct neuronal  differentiations); one-way ANOVA, post hoc Tukey's multiple comparisons test **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"CLN3: 1201
MCOLN1: 57192"
Figure legend: "Cortical neurons were differentiated from iPSCs, generating lysosomal storage disease neurons  and isogenic controls. (H-I)  Electron microscopy analysis of neuronal rosettes (neuronal progenitor cells, NPC) treated with DMSO or TPC2-A1-P. TPC2-A1-P treatment significantly decreased the number of inclusion bodies (black arrow heads) in MCOLN1IVS3-2A&gt;G. CLN3D416G and CLN3ΔEx4-7 lacked an appropriate assay window and showed no significant accumulation of  inclusion bodies. However, CLN3ΔEx4-7 NPC  showed significantly more mitochondria  with aberrant cristae  numbers (white arrow heads), a phenotype which was rescued by TPC2-A1-P (30 µM) treatment. Statistics: Shown are mean values ± SEM. n &gt; 3 technical and biological replicates for each tested condition (each dot represents an imaged frame containing several cells, obtained from at least three distinct neuronal  differentiations); one-way ANOVA, post hoc Tukey's multiple comparisons test **p-value &lt; 0.01; ***p-value &lt; 0.001; ****p-value &lt; 0.0001."
NCBI gene IDs:
"CLN3: 1201
MCOLN1: 57192"
Figure legend: "(D) A cDNA array was used to map TPC2  transcripts in the human brain."
NCBI gene IDs:
"TPC2: 219931"
Figure legend: "Electropherograms of the XRCC4 genomic region encompassing the nucleotide substitutions in available members of the family."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "Quantitative real-time PCR of XRCC4 relative to GAPDH mRNA in affected (II-1 and II-2) and control (CT1 and CT2) fibroblasts. Each value refers to the mean of three independent experiments performed in duplicate. Error bars indicate the standard deviation."
NCBI gene IDs:
"GAPDH: 
XRCC4: 7518"
Figure legend: "γ-H2AX nuclear foci in XRCC4wt and mutant fibroblasts (XRCC4m/m1 and XRCC4m/m2) were determined at different times after irradiation with 0.5 Gy of γ-rays. Values, subtracted of their non-irradiated control (1.3 foci/nucleus in XRCC4wt cells and 1.4 and 0.9 foci/nucleus in XRCC4m/m1 and XRCC4m/m2 cells, respectively), are the mean ± SD of three independent experiments.Percentages of γ-H2AX foci in XRCC4wt and XRCC4m/m fibroblasts (pooled values) remaining at the indicated time-points.Immunofluorescence micrographs of γ-H2AX foci in XRCC4wt and XRCC4m/m fibroblasts.Data information: ***P &lt; 0.001; **P &lt; 0.01; *P &lt; 0.05 (XRCC4m/m vs. XRCC4wt cells), two-way ANOVA, Bonferroni post hoc test."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "A, B Percentages of γ-H2AX nuclear foci remaining in CENP-F-negative (A) and CENP-F-positive (B) cells at the indicated time-points after irradiation with 0.5 Gy of γ-rays. Values, subtracted of their non-irradiated controls, are the mean ± SD of three independent experiments. ***P &lt; 0.001; *P &lt; 0.05 (XRCC4m/m versus XRCC4wt cells), two-way ANOVA, Bonferroni post hoc test.C Immunofluorescence micrographs of γ-H2AX foci in CENP-F-positive and negative fibroblasts at 2 h after irradiation. Arrow heads indicate the CENP-F-positive cells."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "RAD51 foci in XRCC4wt and XRCC4m/m fibroblasts irradiated with 0.5 Gy of γ-rays. Values are the mean ± SD of three independent experiments. ***P &lt; 0.001; **P &lt; 0.01 (XRCC4m/m versus XRCC4wt cells), two-way ANOVA, Bonferroni post hoc test.Immunofluorescence micrographs of RAD51 foci in XRCC4wt and XRCC4m/mfibroblasts. Arrowheads indicate the CENP-F-positive cells."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "XRCC4wt and XRCC4m/m cells irradiated with 0.5 Gy of γ-rays were exposed to the PARP1 inhibitor 3′-AB or to solvent (DMSO). The γ-H2AX foci number is the mean ± SD of three independent experiments. *P &lt; 0.05 (XRCC4m/m + 3′-AB versus XRCC4m/m + DMSO), ***P &lt; 0.001 (XRCC4m/m + 3′-AB versus XRCC4wt + 3′-AB), two-way ANOVA, Bonferroni post hoc test."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "A, B Percentages of DSBs repaired in increasing time intervals (0.5-2 h, 0.5-6 h, 0.5-24 h) were calculated in XRCC4wt (A) and XRCC4m/m (B) cells from the number of γ-H2AX foci in presence or absence of a PARP-1 inhibitor, 3′-AB. **P &lt; 0.01 (XRCC4m/m + 3′-AB versus XRCC4m/m + DMSO), two-way ANOVA, Bonferroni post hoc test."
NCBI gene IDs:
"XRCC4: 7518"
Figure legend: "D) Immunofluorescent images of crypts from CAG-LC3-GFP-RFP and Lgr5-GFP mice 6h post-irradiation. Sections were stained for γH2AX, E-cadherin, and either LC3-RFP or Lgr5-GFP respectively. E) Quantification of the number of γH2AX foci in cells with RFP+ puncta, without autophagic puncta, or in Lgr5+ CBCs. N=3 mice/group, 40-80 cells quantified/mouse."
NCBI gene IDs:
"GFP: 
RFP: 
Lgr5: 14160
LC3: 67443///66734"
Figure legend: "A LC3 punctum formation in WT and GCN5 KO HeLa cells (Scale bars, 10 µm)."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "Immunoblot showing LC3-II formation in WT or GCN5 KO HeLa cells in the presence or absence of the lysosome inhibitor Baf."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "D Formation of LC3 puncta in GCN5 KO HeLa cells overexpressing Myc-tagged GCN5 or GCN5-E575Q (Scale bars, 10 µm)."
NCBI gene IDs:
"Myc: 
GCN5: 2648"
Figure legend: "Formation of LC3 puncta in HeLa cells overexpressing GFP-GCN5 with or without Baf treatment"
NCBI gene IDs:
"GFP: 
GCN5: 2648"
Figure legend: "Formation of LC3 puncta in HeLa cells overexpressing GFP-GCN5 with or without Baf treatment (Graph represents data from three independent experiments with ≥ 30 cells per condition; mean ± SEM; *p &lt; 0.05, **p &lt; 0.01, Student's t-test; Scale bar, 10 µm)."
NCBI gene IDs:
"GFP: 
GCN5: 2648"
Figure legend: "H LC3-II formation in GFP-GCN5-overexpressing HeLa cells."
NCBI gene IDs:
"GFP: 
GCN5: 2648"
Figure legend: "I GFP-p62 levels in HEK293 cells stably expressing GFP-p62. The cells were cultured with GCN5 siRNA with or without CQ."
NCBI gene IDs:
"GFP: 
GCN5: 2648
p62: 8878"
Figure legend: "J PDLIM1 and IFT20 protein levels in GCN5 KO HEK293 cells with or without transfection of GFP-GCN5 and addition of CQ."
NCBI gene IDs:
"GFP: 
GCN5: 2648"
Figure legend: "K Representative images of mCherry-Atg8a (red) and DAPI (blue) in Drosophila larval fat body in which dGcn5 is overexpressed (OE) or silenced (KD) using the pan-fat body driver (cg-GAL4). Drosophila (cg-GAL4/+) was used as the control (Graph represents data from three independent experiments with ≥ 30 cells per condition; mean ± SEM; *p &lt; 0.05, ***p &lt; 0.001, Student's t-test; Scale bars, 10 µm)."
NCBI gene IDs:
"GAL4: 855828
dGcn5: 39431"
Figure legend: "A LAMP1 puncta (green) and DAPI (blue) in WT and GCN5 KO HEK293 cells (Scale bars, 10 µm)."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "B Fluorescence-activated cell sorting analysis of WT and GCN5 KO HEK293 cells stained with LysoTracker. Fluorescence intensity of 10,000 cells per sample was measured."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "C Immunoblot showing lysosomal protein levels in three independent clones of GCN5 KO HEK293 cells. CTSD HC, cathepsin D heavy chain."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "D LAMP1 puncta in GCN5 KO HEK293 cells overexpressing Myc-tagged GCN5 or GCN5-E575Q (Scale bars, 10 µm)."
NCBI gene IDs:
"Myc: 
GCN5: 2648"
Figure legend: "F Hexosaminidase activity in GCN5 KO HEK293 cells (mean ± SEM; n = 3 independent experiments; ***p &lt; 0.001, Student's t-test)."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "G Degradation of EGFR in WT and GCN5 KO HEK293 cells in the presence or absence of CQ."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "H Representative images of LysoTracker staining (red) and DAPI (blue) in Drosophila larval fat body in which dGcn5 is overexpressed (OE) or silenced (KD). Drosophila (cg-GAL4/+) was used as the control (Graph represents data from three independent experiments with ≥ 30 cells per condition; mean ± SEM; *p &lt; 0.05, **p &lt; 0.01, Student's t-test; Scale bars, 10 µm)."
NCBI gene IDs:
"GAL4: 855828
dGcn5: 39431"
Figure legend: "A Quantification of LC3 puncta in WT, GCN5 KO and GCN5/TFEB DKO HeLa cells in the presence or absence of Baf (Graph represents data from three independent experiments with ≥ 30 cells per condition; mean ± SEM; ***p &lt; 0.001, Student's t-test)."
NCBI gene IDs:
"GCN5: 2648
TFEB: 7942"
Figure legend: "B Immunoblot showing LC3-II formation in WT, GCN5 KO and GCN5/TFEB DKO HeLa cells in the presence or absence of Baf."
NCBI gene IDs:
"GCN5: 2648
TFEB: 7942"
Figure legend: "C Quantification of LAMP1 puncta in WT, GCN5 KO and GCN5/TFEB DKO HEK293 cells (Graph represents data from three independent experiments with ≥ 30 cells per condition; mean ± SEM; ***p &lt; 0.001, Student's t-test)."
NCBI gene IDs:
"GCN5: 2648
TFEB: 7942"
Figure legend: "D Immunoblot showing LAMP1 levels in WT, GCN5 KO and GCN5/TFEB DKO HEK293 cells."
NCBI gene IDs:
"GCN5: 2648
TFEB: 7942"
Figure legend: "E Acetylation of TFEB-Flag in stable TFEB-Flag-expressing HEK293 cells with overexpression of individual histone acetyltransferases as indicated. TFEB-Flag was immunoprecipitated with anti-Flag beads and analyzed by immunoblot using anti-acetyl-lysine (Ace-lys)."
NCBI gene IDs:
"Flag: 
TFEB: 7942"
Figure legend: "F TFEB-Flag acetylation in the stable TFEB-Flag-expressing HEK293 cells transfected with Myc-tagged GCN5 or GCN5-E575Q after incubation with GCN5 siRNA for 48 h."
NCBI gene IDs:
"Flag: 
Myc: 
GCN5: 2648
TFEB: 7942"
Figure legend: "J Acetylation of Flag-tagged TFEB or TFEB mutants expressed in HEK293 cells. 3KR: Lys 116, Lys 274, and Lys 279 were replaced by Arg."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "A RT-qPCR analysis of the expression of TFEB target genes in WT, GCN5 KO and GCN5/TFEB DKO HEK293 cells. Data information: In this Figure, data are presented as mean ± SEM; n = 3 independent experiments; *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Student's t-test."
NCBI gene IDs:
"GCN5: 2648
TFEB: 7942"
Figure legend: "B RT-qPCR analysis of dMitf target gene expression in Drosophila larval fat body with silencing of dGcn5. Data information: In this Figure, data are presented as mean ± SEM; n = 3 independent experiments; *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Student's t-test."
NCBI gene IDs:
"dGcn5: 39431
dMitf: 3885647"
Figure legend: "C Expression of TFEB target genes in HEK293 cells transfected with or without TFEB-WT or TFEB-3KR. Data information: In this Figure, data are presented as mean ± SEM; n = 3 independent experiments; *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Student's t-test."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "D, E Luciferase activity measured in WT, GCN5 KO and GCN5/TFEB DKO HEK293 cells (D), and in HEK293 cells with TFEB-WT or TFEB-3KR transfection (E). The cells were transfected or co-transfected with a TFEB-luciferase reporter. 3KR: Lys 116, Lys 274, and Lys 279 were replaced by Arg. Data information: In this Figure, data are presented as mean ± SEM; n = 3 independent experiments; *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Student's t-test."
NCBI gene IDs:
"luciferase: 
GCN5: 2648
TFEB: 7942"
Figure legend: "A Subcellular localization of TFEB in WT or GCN5 KO HEK293 cells treated with or without Torin1 (Scale bars, 10 µm)."
NCBI gene IDs:
"GCN5: 2648"
Figure legend: "C ChIP-qPCR analysis of TFEB binding to the promoter of its target genes CLCN7, GLA and CTSD. Normal mouse IgG and primers against the upstream region lacking CLEAR sites (up) were used as negative controls (mean ± SEM; n = 3 independent experiments; *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Student's t-test)."
NCBI gene IDs:
"CLCN7: 1186
CTSD: 1509
GLA: 2717
TFEB: 7942"
Figure legend: "D Electrophoresis mobility shift assay of TFEB binding to the promoter of GLA. A DNA fragment from the GLA promoter containing the TFEB binding site was incubated with purified recombinant TFEB or each of the TFEB mutants, and was subjected to electrophoresis. Asterisk indicates the DNA fragment from the GAPDH promoter which was used as a TFEB non-binding DNA control."
NCBI gene IDs:
"GAPDH: 2597
GLA: 2717
TFEB: 7942"
Figure legend: "I TFEB homodimer formation detected by glutaraldehyde (GA) crosslinking. Purified recombinant GST-tagged TFEB or TFEB mutants was incubated with or without glutaraldehyde, then the products were analyzed by immunoblotting using anti-TFEB."
NCBI gene IDs:
"GST: 
TFEB: 7942"
Figure legend: "E LC3-II formation in HeLa cells transfected with Flag-tagged TFEB or TFEB mutants after TFEB RNAi. The cells were treated with or without amino acid starvation."
NCBI gene IDs:
"Flag: 
TFEB: 7942"
Figure legend: "F GFP-p62 levels in HEK293 cells stably expressing GFP-p62. The cells were treated with or without addition of CQ."
NCBI gene IDs:
"GFP: 
p62: 8878"
Figure legend: "Representative images of mCherry-Atg8a (red) (G) in Drosophila larval fat body in which dMitf or dMitf-2KQ (K445Q/K450Q) was overexpressed. Drosophila (cg-GAL4/+) was used as the control and DAPI staining (blue) was used to indicate the cell nucleus (Scale bars, 10 µm)."
NCBI gene IDs:
"GAL4: 855828
dMitf: 3885647"
Figure legend: "Representative images of LysoTracker (red) staining (H) in Drosophila larval fat body in which dMitf or dMitf-2KQ (K445Q/K450Q) was overexpressed. Drosophila (cg-GAL4/+) was used as the control and DAPI staining (blue) was used to indicate the cell nucleus (Scale bars, 10 µm)."
NCBI gene IDs:
"GAL4: 855828
dMitf: 3885647"
Figure legend: "The level of insoluble Tau protein in the head of adult Drosophila with or without CQ feeding. The indicated genes were transgenically expressed in the eyes of the Drosophila using the GMR-Gal4 driver. Drosophila (GMR-Gal4/+) was used as the control."
NCBI gene IDs:
"GMR: 
Gal4: 855828"
Figure legend: "C Representative light micrographs of adult Drosophila eyes expressing the indicated transgenes under the control of the GMR-Gal4 driver. The Drosophila were fed with or without CQ. Drosophila (GMR-Gal4/+) was used as the control."
NCBI gene IDs:
"GMR: 
Gal4: 855828"
Figure legend: "D. Tyrosine phosphorylation of LORE in vivo. Two-week-old 35S:LORE-FLAG complementation plants were subjected to immunoprecipitation with anti-FLAG agarose beads. A23 (40 μM) was used to inhibit the protein phosphorylation, and the treatments were same as in (A). Protein abundance of precipitated LORE-FLAG was detected by immunoblotting. The tyrosine phosphorylation was probed by anti-pTyrosine (α-pY) antibody. Similar results were observed in three biological replicates."
NCBI gene IDs:
"FLAG: 
LORE: 842432"
Figure legend: "B. Y600 mutation reduced LORE kinase activity. MBP-tagged LORE-CD and LORE-Y600F-CD (Y600 tyrosine was substituted with phenylalanine, F) were subjected to kinase reactions. The kinase dead mutant LOREK516E served as a negative control. Autophosphorylated LORE was visualized by autoradiography following SDS-PAGE gel electrophoresis. Protein abundance was stained by CBB."
NCBI gene IDs:
"LORE: 842432"
Figure legend: "C. LORE Y600 is phosphorylated in plants. Two-week-old pLORE:LORE-FLAG complementation plant leaves were subjected to immunoprecipitation (IP) with anti-FLAG agarose beads. Y600 specific phosphorylation antibody was used to detect Y600 phosphorylation in LORE. The experiment was repeated three times with similar results."
NCBI gene IDs:
"FLAG: 
LORE: 842432"
Figure legend: "D. 3-OH-C10:0 induced Y600 phosphorylation. Two-week-old pLORE:LORE-FLAG complementation plants were treated with 1 μM 3-OH-C10:0 at indicated times. Plant leaves were subjected to anti-FLAG IP. Phosphorylation level of Y600 was detected by anti-pY600 antibody. The experiment was repeated three times with similar results."
NCBI gene IDs:
"FLAG: 
LORE: 842432"
Figure legend: "E. Y600 mutation abolished the ROS burst triggered by 3-OH-C10:0. The transgenic plants were treated with 3-OH-C10:0 and ROS burst was measured. WT(6-1) and WT(10-3) are two independent complementation lines with LORE; Y600F(2-6) and Y600F(7-1) are two independent lines complemented with LOREY600F; and kinase-dead LOREK516E was transformed to lore mutant as a negative control. The 2-3 and 5-2 are two independent lines. Values are means ± SD (n=6 biological replicates). (ANOVA, P &lt;0.01)."
NCBI gene IDs:
"lore: 842432
LORE: 842432"
Figure legend: "A. LORE interacts with specific RLCKs (PBL34/35/36) in yeast. BD-LORE-CD and AD-PBLs were co-transformed to yeast cells and were screened on synthetic dextrose media lacking leucine and tryptophan (SD/-Leu-Trp). The single yeast colonies were serially diluted onto SD/-Leu-Trp and SD/-Leu-Trp-His (SD media lacking leucine, tryptophan and histidine) to observe yeast cell growth. 3-AT(20 mM) was used to inhibit the autoactivation of LORE. Yeast co-transformed with pGADT7-T+pGBKT7-53(+) served as a positive control, and the yeast co-transformed with pGADT7-T+pGBKT7-lam(-) and AD-PBL39+BD-LORE served as the negative controls. EV, empty vector."
NCBI gene IDs:
"GADT7: 
GBKT7: 
LORE: 842432"
Figure legend: " C. Co-immunoprecipitation (Co-IP) of LORE with PBLs. 35S promoter-driven LORE-T7 and PBLs-FLAG were transiently co-expressed in N. benthamiana by agroinfiltration. Plant leaves were immunoprecipated with anti-FLAG beads and the proteins were immunoblotted by anti-FLAG antibody. Co-IP proteins were immunoblotted by anti-T7 antibody. The experiment was repeated three times with similar results. "
NCBI gene IDs:
"35S: 
PBL: "
Figure legend: " D. LORE interacts with PBLs, revealed by split-luciferase assays. N. benthamiana leaves were co-infiltrated with Agrobacteria carrying 35S:LORE-nLUC and 35S:cLUC-PBLs. Images of chemiluminescence were obtained by applying 0.5 mM luciferin 36 h post infiltration. Similar results were observed in three biological replicates. "
NCBI gene IDs:
"35S: 
PBL: 
LORE: 842432"
Figure legend: " A. ROS burst in pbl34/35/36 triple mutant, induced by 3-OH-C10:0. Two-week-old seedlings were treated with 1 μM 3-OH-C10:0, and the ROS burst was measured. Values are means ± SD (n=6 biological replicates). (ANOVA, P &lt;0.01). "
NCBI gene IDs:
"pbl34: 831360"
Figure legend: " C. pbl34/35/36 triple mutant displayed decreased callose depositions in response to 3-OH-C10:0 treatment. Two-week-old seedlings were sprayed with 1 μM 3-OH-C10:0. The leaves were sampled for callose deposition assays 12 hrs later. The stained callose was counted for 1 mm2 of the leaves. Mock is solvent control. Scale bar=100μm.Values are means ± SD (n=6 leaves). (Student's t-test, **P&lt; 0.01). "
NCBI gene IDs:
"pbl34: 831360"
Figure legend: " D. Root length of pbl34/35/36 triple mutant treated with 3-OH-C10:0. The Arabidopsis seedlings were grown on ½ MS containing 2.5 μM 3-OH-C10:0 for six days, and the root length was measured. Scale bar=0.4cm. Values are means ± SD (n=3 biological replicates). (ANOVA, P &lt;0.01). "
NCBI gene IDs:
"pbl34: 831360"
Figure legend: " E. Disease phenotype of pbl34/35 double, pbl34/35/36 triple mutants and PBL34 overexpression plants. 35S:PBL34-FLAG was introduced to the Col-0 to generate the overexpression lines. The homozygous transgenic plants were used for the assays. The plants were sprayed with Pst DC3000 at a concentration of 1×109 cfu/ml. Plants were subjected to bacterial growth curve analysis at 3 dpi. OE-3# and OE-11# are two independent transgenic lines. Values are means ± SD (n=6 biological replicates). (ANOVA, P &lt;0.01). "
NCBI gene IDs:
"35S: 
FLAG: 
pbl34: 831360
PBL34: 831360"
Figure legend: " F. pbl34/35/36 triple mutant phenotype was rescued by PBL34. PBL34-HA was introduced to the triple mutant driven by its native promoter. Four-week-old homozygous seedlings were inoculated with 5×104 cfu/ml Pst DC3000 , and the leaves were subjected to bacterial growth curve analysis at 3 dpi. CM2-5 and CM7-3 are two independent transgenic lines. Values are means ± SD (n=6 biological replicates). (ANOVA, P &lt;0.01). "
NCBI gene IDs:
"PBL34: 831360
pbl34: 831360"
Figure legend: " B. Mutation of PBL34 T306/T310 significantly reduced its phosphorylation by LORE. Recombinant kinase dead PBL34* and PBL34*-2A (T306/T310 was mutated to A) were incubated with LORE-CD. Phosphorylation of PBL34 was visualized by 32P autoradiography. Protein abundance was revealed by CBB staining. "
NCBI gene IDs:
"PBL34: 831360"