EMBO/SourceData huggingface dataset:
Figure legend: "C Quantitative RT-PCR analysis of BMP2-induce Sp7 transcription. Prx1-Cre;Usp34f/f MSCs were treated with Smurf1 or control siRNAs, and depleted from serum overnight before BMP2 stimulation. Results are shown as mean ± SEM; n=3; *: p&lt;0.05 and **: p&lt;0.01 by ANOVA with Tukey's post hoc test"
NCBI gene IDs:
"Prx1: 
Smurf1: 75788
Sp7: 170574
Usp34: 17847"
Figure legend: " F Representative images of ALP and ARS staining. Mouse MSCs were treated with Smurf1 or control siRNAs, and cultured in osteogenic medium for 5 and 10 days, respectively"
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "I, J Quantitative RT-PC analyses of the expression of osteogenic markers. Cells were treated with Smurf1 or control siRNAs, and cultured in osteogenic medium for 5 days. Results are shown as mean ± SEM; n=3; *: p&lt;0.05, **: p&lt;0.01 and ***: p&lt;0.001 by ANOVA with Tukey's post hoc test"
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "western blot analyses of the expression of osteogenic markers. Cells were treated with Smurf1 or control siRNAs, and cultured in osteogenic medium for 5 days. Results are shown as mean ± SEM; n=3; *: p&lt;0.05, **: p&lt;0.01 and ***: p&lt;0.001 by ANOVA with Tukey's post hoc test"
NCBI gene IDs:
"Smurf1: 75788"
Figure legend: "(F) SCD1 overexpression affects RNF145/ADIPOR2. HEK-293T cells containing an empty control vector or expressing SCD1-HA were cultured in FCS-free DMEM for 24h and whole-cell lysates analysed. * non-specific bands."
NCBI gene IDs:
"HA: 
SCD1: 6319"
Figure legend: "(A) Endogenous ADIPOR2 is stabilised in RNF145 knockout cells. Representative 35S pulse-chase analysis in HeLa WT, RNF145 knockout (∆RNF145) or ADIPOR2 knockout (∆AR2) cells. Quantification of 35S-labelled ADIPOR2 normalised to t = 0 h is shown below."
NCBI gene IDs:
"ADIPOR2: 79602
AR2: 79602
RNF145: 153830"
Figure legend: "(B) ADIPOR2 regulation requires the E3 ligase activity of RNF145. HEK-293T RNF145 knockout cells (∆RNF145) were stably complemented with WT RNF145 (RNF145-V5) or an inactive RNF145C552A,H554A RING domain mutant (mRNF145-V5). * non-specific bands."
NCBI gene IDs:
"V5: 
RNF145: 153830"
Figure legend: "(C) RNF145 binds ADIPOR2. RNF145 was immunoprecipitated from HeLa RNF145 knockout cells complemented with WT RNF145 (RNF145-V5) or an inactive RNF145C552A,H554A RING domain mutant (mRNF145-V5). Cells were treated with VCP inhibitor (NMS-873, 10 µM, 90 min) prior to immunoprecipitation with an anti-V5 antibody."
NCBI gene IDs:
"V5: 
RNF145: 153830"
Figure legend: "(D) The E2 ubiquitin-conjugating enzyme UBE2G2 is involved in RNF145-mediated ADIPOR2 degradation. Immunoblot analysis of HEK-293T cells depleted of RNF145 (gRNF145), UBE2G2 (gUBE2G2) or B2M (control; gCTR)."
NCBI gene IDs:
"B2M: 567
RNF145: 153830
UBE2G2: 7327"
Figure legend: "(E) ADIPOR2 degradation depends on UBXD8. HEK-293T cells were depleted of B2M (gCTR) or UBXD8 (gUBXD8) and analysed by immunoblotting."
NCBI gene IDs:
"B2M: 567
UBXD8: 23197"
Figure legend: "(B) UBXD8 participates in PA-modulated RNF145 degradation. CRISPR/Cas9-mediated depletion of UBXD8 (gUBXD8) or B2M (gB2M, control) in HEK-293T cells, followed by exposure to PA (400 µM, 6h) or BSA (6h, vehicle control)."
NCBI gene IDs:
"CRISPR: 
B2M: 567
Cas9: 69900935
UBXD8: 23197"
Figure legend: "(C) PA and OA treatment alter RNF145 ubiquitination. Immunoprecipitation of RNF145 from cells treated with BSA (control), PA or OA (each 200 µM, 3h). During FA treatments, VCP inhibitor (NMS-873, 10 µM) was present to stabilise ubiquitinated proteins. RNF145 ubiquitination (ubi) was detected using an anti-ubiquitin antibody (VU-1)."
NCBI gene IDs:
"RNF145: 153830"
Figure legend: "(E) Lysine-less RNF145 is stabilised under PA treatment. RNF145 knockout HEK-293T cells (∆RNF145) were complemented with RNF145-V5 or a lysine-less variant of RNF145 (RNF145-V5 K &gt; R) and treated BSA or PA (400 µM, 6h). Dotted lines demarcate the different cell lines."
NCBI gene IDs:
"V5: 
RNF145: 153830"
Figure legend: "(F) Adjustment of RNF145 levels to PA requires its ubiquitin ligase activity. Immunoblot of whole-cell lysates from RNF145 knockout HEK-293T (∆RNF145) cells stably complemented with RNF145-V5 or RNF145C552A, H554A-V5 (mRNF145-V5) and treated with BSA or PA (400 µM, 6h). LE, long exposure."
NCBI gene IDs:
"V5: 
RNF145: 153830"
Figure legend: "(A) ADIPOR2 regulation by FAs is completely dependent on RNF145. WT or RNF145 knockout HEK-293T cells (∆RNF145) were treated with BSA, PA or OA (400 µM, 6h) and analysed by immunoblotting. Non-specific bands are indicated (*)."
NCBI gene IDs:
"RNF145: 153830"
Figure legend: "(B) RNF145 - ADIPOR2 interaction is FA-modulated. Immunoprecipitation of endogenous RNF145 HEK-293T cells depleted of UBE2G2 (gUBE2G2) and/or RNF145 (gRNF145) and treated with 400 µM of the indicated PA, OA or BSA (control) for 3h. LE, long exposure. * non-specific bands."
NCBI gene IDs:
"RNF145: 153830
UBE2G2: 7327"
Figure legend: "(C) Immunoprecipitation (IP) of endogenous ADIPOR2 from WT or ∆RNF145 cells (see (C)) treated with MG132 (5 µg/ml, 6h) ± OA (200 µM, 6h). Ubiquitinated ADIPOR2 was detected using a specific anti-ubiquitin antibody (clone VU-1). Note that ADIPOR2 levels in RNF145 knockout HEK-293T cells are increased."
NCBI gene IDs:
"RNF145: 153830"
Figure legend: "(D) ADIPOR2 knockout HEK-293T cells were stably complemented with ADIPOR2-HA, an ADIPOR2 mutant in which all lysines were mutated to arginines (K-less ADIPOR2-HA), or an empty vector control and exposed to OA (200 µM, 6h) or BSA (control). ADIPOR2 variants were visualised by immunoblotting using a specific anti-HA antibody. LE, long exposure."
NCBI gene IDs:
"HA: 
ADIPOR2: 79602"
Figure legend: "(A) Loss of ADIPOR2 sensitises cells to PA-induced cytotoxicity. HEK-293T cells were depleted of RNF145 (purple), ADIPOR2 (blue), B2M (CTR; grey), or both ADIPOR2 and RNF145 (blue + purple), exposed to PA (200 µM, 96h) and stained with crystal violet. Quantification of crystal-violet stained cells is shown alongside a representative micrograph (below). The response of each cell line to PA was normalised to its BSA-treated condition (dotted line). Statistically significant differences to cells containing gCTR cells are indicated (robust one-way ANOVA with Benjamini-Hochberg multiple testing correction). Mean ± SD are shown. Boxplots represent the median, first and third quantiles. Upper and lower whiskers extend to values up to 1.5-fold interquartile range. N = 3 biological replicates, ** q ≤ 0.01. Double knockdowns were generated by sequential constitutive CRISPR/Cas9-mediated depletion of RNF145 or ADIPOR2. Knockdown efficiencies are shown in Appendix Fig S1A."
NCBI gene IDs:
"CRISPR: 
ADIPOR2: 79602
B2M: 567
Cas9: 69900935
RNF145: 153830"
Figure legend: "(C) HEK-293T cells depleted of B2M (gCTR) or ADIPOR2 (gADIPOR2) were complemented with empty vector (blue), full-length ADIPOR2-HA (green), or a catalytically inactive variant of ADIPOR2-HA (dead ADIPOR2; red) and treated with PA (200 µM, 96h) or BSA (vehicle control, 96h). Measurements from PA-treated cells were normalised to their BSA-treated condition (dotted line). Cell growth was quantified, and representative crystal violet staining is shown. Boxplots represent the median, first and third quantiles. Upper and lower whiskers extend to values up to 1.5-fold interquartile range. ** q ≤ 0.01 (robust one-way ANOVA with Benjamini-Hochberg multiple testing correction)."
NCBI gene IDs:
"HA: 
ADIPOR2: 79602
B2M: 567"
Figure legend: "(E) Reduced glutathione (GSH) partially blocks mitochondrial fission. Neurons were transfected with Mito pretreated for 2 h with 2 mM GSH monoethyl ester, washed once and then exposed to 35 μM SNOC. Mitochondrial fission was scored after 1 h. Data are means±s.e.m. of quintuplicate samples from one representative experiment of a total of three (†††significance at P0.001 or n.s., †not significant compared to control; *significance at P0.05)."
NCBI gene IDs:
"Mito: "
Figure legend: "(B) Percentage of mitochondrial fission and cell death at 18 h († and ††† significance at P0.05 and 0.001 as compared to control pcDNA3 transfection; *,** and *** significance at P0.05 , 0.01 and 0.001, respectively, compared to SNOC treated, pcDNA3 transfected neurons; n=3 independent experiments). Dying neurons were recognized by their shrunken and condensed nuclei after Hoechst 33342 staining."
NCBI gene IDs:
"pcDNA3: "
Figure legend: "(C) Enforced Drp1 or Fis1 expression (60 h) evokes fission and neuronal cell death (*,** and *** significance at P0.05, 0.01 and 0.001, respectively, compared to pcDNA3 transfected neurons; n=3 independent experiments)."
NCBI gene IDs:
"pcDNA3: 
Drp1: 114114
Fis1: 288584"
Figure legend: "(E) Mfn1 or Drp1K38A inhibits 100 nM rotenone‐induced mitochondrial fission (4 h) and cell death (48 h). Data indicate means±s.e.m. of triplicate measurements (†† and ††† significance at P0.01 and 0.001 as compared to untreated control; ***significance at P0.001 as compared to rotenone treatment with pcDNA3 transfection; n=3)."
NCBI gene IDs:
"pcDNA3: 
Drp1: 114114
Mfn1: 192647"
Figure legend: "(F) Mitochondrial fission by Aβ35-25 or Aβ25-35 exposure. The fraction of neurons exhibiting fragmented mitochondria is shown as the mean±s.e.m. (††significance at P0.01 compared to pcDNA3 transfected, Aβ35−25 treated control; * and ** significance at P0.05 and 0.001, respectively, compared to pcDNA3 transfected, Aβ25-35 treated neurons; n=3)."
NCBI gene IDs:
"pcDNA3: "
Figure legend: "(B) qPCR  analysis of Nrf1, Gabpa  and Ppargc1a  mRNA in mouse PMs  infected with VSV  or HSV-1 or treated with poly(I:C) or ISD  for the indicated time points (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"Gabpa: 14390
Nrf1: 18181
Ppargc1a: 19017"
Figure legend: "(C) Immunoblot  analysis of NRF1  expression in mouse PMs  transfected with p65-specific siRNA (si-p65) or control siRNA (si-NC) for 48 h, followed by infection with VSV  or HSV-1 for 8 h. GAPDH  was used as a loading control. Ratios of target proteins versus loading control normalized to the untreated sample of each condition."
NCBI gene IDs:
"p65: 20979"
Figure legend: "(E) Immunoblot  analysis of mitochondrial proteins and autophagy marker LC3  in mouse PMs  transfected with NRF1-specific siRNA (si-NRF1) or control siRNA (si-NC) for 48 h, followed by infection with VSV  or HSV-1 for the indicated time points. ACTIN was used as a loading control. Ratios of target proteins versus loading control normalized to the untreated sample of each condition."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(F) Mitochondrial mass  determined by mtDNA/nuclear DNA (nDNA) ratio in mouse PMs  transfected with NRF1-specific siRNA (si-NRF1) or control siRNA (si-NC) for 48 h, followed by infection with VSV  or HSV-1  for the indicated time points (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "RNA-sequencing  (RNA-seq) analysis of  gene expression in wild-type and NRF1-KO mouse PMs  (n=3 biological replicates per group) infected with VSV  for 8 h. (C) Pheatmap analysis showing downregulated DEGs encoding  mitochondrial inner membrane  proteins. TPM, transcripts per kilobase of exon model per million mapped reads."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(D) Mitochondrial mass  determined by mtDNA/nuclear DNA (nDNA) ratio in wild-type and NRF1-KO mouse PMs  infected with VSV  or HSV-1  for the indicated  time points (n=3 biological replicates per group). Data information: Data are from three independent experiments (D) and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(F and G) The representative curves of oxygen consumption rate  (OCR) in wild-type and NRF1-KO mouse PMs  infected with VSV  (F) or HSV-1 (G) for 8 h (n=5 biological replicates per group). Oligomycin: 1 µM, FCCP: 4 µM, Rotenone: 1 µM. Quantification of basal and maximal OCR. Data information: Data are from at least three biological replicates and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(H and I) Electron micrographs  of mitochondria  in wild-type and NRF1-KO mouse PMs  infected with VSV or HSV-1 for 8 h. Scale bars, 500 nm (H). (I) Quantitative analysis of mitochondrial cristae (the ratio of cristae  number to mitochondrial  area) in (H) (20-30 mitochondria  per group, biological replicates). Data information: Data are from at least three biological replicates and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(A) Luciferase activity of IFN-β, NF-κB and ISRE  promoter reporter in HEK293T  cells transfected with empty vector or plasmids encoding incremental NRF1-Myc  for 24 h followed by infection with VSV  for 8 h (n=4 biological replicates per group)."
NCBI gene IDs:
"Myc: 
IFN-β: 3456
NRF1: 4899"
Figure legend: "(B and C) Immunoblot  analysis of phosphorylated  and total TBK1, p65  and STAT1  in wild-type and NRF1-KO mouse PMs  infected with VSV  or HSV-1  (B) or stimulated with poly(I:C) or ISD  (C) for the indicated time points. GAPDH  was used as a loading control. Ratios of phosphorylated  proteins versus total proteins normalized to the 0-h or 4-h  time point of each wild-type sample."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(D) qPCR  analysis of Ifnb1, Il6, Tnfa  and Cxcl10  mRNA in wild-type and NRF1-KO mouse PMs  infected with VSV  or HSV-1  or stimulated with  poly(I:C)  or ISD  for the indicated time points (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ND, not detectable, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"Cxcl10: 15945
Ifnb1: 15977
Il6: 16193
NRF1: 18181
Tnfa: 21926"
Figure legend: "(E) ELISA  quantification of  IFN-β, IL6  and TNF-α secretion in wild-type and NRF1-KO mouse PMs  treated as in (D) (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ND, not detectable, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(A) 8-week-old Nrf1F/F and Nrf1MyeKO mice  (n=3 for PBS  treatment and n=6 for virus treatment) were intravenously infected with VSV  (4×107 PFU/mouse) or HSV-1 (1×107 PFU/mouse). After 24 h, ELISA  quantification of IFN-β, IL6  and TNF-α in sera. Data information: Data are presented as mean ± s.e.m. Two-tailed, unpaired Student's t test were used to measure significance. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Nrf1: 18181"
Figure legend: "(B) qPCR  analysis of Ifnb1, Il6  and Tnfa  mRNA in the lung  of Nrf1F/F and  Nrf1MyeKO mice  infected with VSV  as in A (n=6 per group). Data information: Data are presented as mean ± s.e.m. Two-tailed, unpaired Student's t test were used to measure significance. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Ifnb1: 15977
Il6: 16193
Nrf1: 18181
Tnfa: 21926"
Figure legend: "(C) qPCR  analysis of VSV  mRNA and  plaque assay of VSV  titers in the lungs  of  Nrf1F/F and Nrf1MyeKO mice  infected with VSV  as in A (n=6 per group). Data information: Data are presented as mean ± s.e.m. Two-tailed, unpaired Student's t test were used to measure significance. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Nrf1: 18181"
Figure legend: "(E) qPCR  analysis of Ifnb1, Il6  and Tnfa  mRNA in the brains  of Nrf1F/F and Nrf1MyeKO mice  infected with HSV-1 as in (A) (n=6 per group). (F) qPCR  analysis of HSV-1 UL30  mRNA and plaque assay  of HSV-1 titers in the brain  of Nrf1F/F and Nrf1MyeKO mice  infected with HSV-1 as in (A) (n=6 per group). Data information: Data are presented as mean ± s.e.m. Two-tailed, unpaired Student's t test were used to measure significance. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Ifnb1: 15977
Il6: 16193
Nrf1: 18181
Tnfa: 21926
UL30: 2703462"
Figure legend: "(G) Survival  of Nrf1F/F and Nrf1MyeKO mice  (n=10 per group) at various times  after intraperitoneal  (i.p.) infection with HSV-1 (2×107 PFU per mouse). Data information: Data are presented as mean ± s.e.m. log-rank (Mantel-Cox) test (G) were used to measure significance. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"Nrf1: 18181"
Figure legend: "(A) mtROS levels measured by MitoSOX staining  and  flow cytometry in wild-type and NRF1-KO mouse PMs  infected with VSV  (left) or HSV-1  (right) for the indicated time points. MFI, mean fluorescence intensity (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(B) DNA was isolated from digitonin  extracts of wild-type and NRF1-KO mouse PMs  infected with VSV  (left) or HSV-1 (right) for the indicated  time points. Cytosolic  translocation of mtDNA  was quantified via qPCR  using the indicated primer sets. 18S rRNA served as a control (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"18S rRNA: 
NRF1: 18181"
Figure legend: "(C and D) Wild-type and NRF1-KO mouse PMs  infected with VSV  (8 h) or HSV-1 (4 h) were imaged by confocal microscope after staining  with anti-DNA  (DNA) and anti-Prohibitin  (Mito) antibodies. Scale bars, 5 µm (C). (D) The number of cytosolic mtDNA  puncta per cell was quantitated by ImageJ (n=30 cells per group). Data information: Data are from three independent experiments or representative data (C) and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(E) ELISA  quantification of IFN-β (left) and IL6  (right) in wild-type and NRF1-KO mouse PMs  pretreated with or without EtBr  (450 ng/ml, 72 h), followed by infection with VSV  or HSV-1 for 8 h (n=3 biological replicates per group). (F) ELISA  quantification of IFN-β (left) and IL6  (right) in wild-type and NRF1-KO mouse PMs  pretreated with or without MitoQ  (100 nM, 2 h), followed by infection with VSV  or HSV-1 for 8 h (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(H) qPCR  analysis of Ifnb1  and Il6  mRNA in the lungs  of Nrf1F/F and Nrf1MyeKO mice  pretreated with MitoQ  as in (G) and 1 h later infected i.p. with VSV  (5×108 PFU/mouse) for 24 h (n=6 per group). (I) qPCR  analysis of Ifnb1  and Il6  mRNA in the brains  of Nrf1F/F and Nrf1MyeKO mice  pretreated with MitoQ  as in (G) and 1 h later infected i.p. with HSV-1 (1×107 PFU/mouse) for 24 h (n=6 per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, using a two-tailed, unpaired Student's t test."
NCBI gene IDs:
"Ifnb1: 15977
Il6: 16193
Nrf1: 18181"
Figure legend: "(G) Endogenous phospho-NRF1  S318 signal was detected in wild-type and NRF1  S318A mouse PMs  after the infection with different viruses for 8 h. The red arrows indicate phosphorylated  signals for NRF1."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(J) ELISA  quantification of  IFN-β and IL6  secretion in wild-type and NRF1  S318A mouse PMs  treated as in (I) (n=3 biological replicates per group). Data information: Data are from three independent experiments and presented as mean ± s.e.m. ns, not significant (p &gt; 0.05), *p &lt; 0.05, **p &lt; 0.01. Two-tailed, unpaired Student's t test were used to measure significance."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "(L) Survival  of wild-type and NRF1  S318A mice  (n=10 per group) at various times  after intraperitoneal infection with HSV-1 (2×107 PFU per mouse). Data information: log-rank (Mantel-Cox) test (L) were used to measure significance."
NCBI gene IDs:
"NRF1: 18181"
Figure legend: "C. WT and mutant 7SK fragment 5 containing U250G were analyzed as in B."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "E. 7SK RNA, generated by either in vitro transcription (IVT) or purified from HeLa total RNA and then site-specifically labeled at the indicated positions by the procedure in D, was analyzed by 1D-TLC."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "F. Cyber Gold staining of 7SK obtained by either IVT or immunoprecipitation (IP) with the indicated antibodies."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "G. 2D-TLC analysis of 32p-labeled mononucleotides derived from IVT or CDK9-bound 7SK RNA, with the first panel depicting a schematic migration pattern of the indicated 5'-phosphorylated mononucleotides."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "H. 7SK RNA, obtained by either IVT or anti-CDK9 IP and then site-specifically labeled at the indicated positions by the procedure in D, was analyzed by 1D-TLC."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "C-F; H-K; and N-Q. The tagged WT or mutant 7SK RNA was co-transfected with the indicated Flag-tagged proteins into HeLa cells. Anti-Flag or immunoprecipitates (IP) were analyzed by immunoblotting for the indicated proteins and primer extension for the bound 7SK RNA."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "C-F; H-K; and N-Q. The tagged WT or mutant 7SK RNA was co-transfected with the indicated Flag--tagged proteins into HeLa cells. Anti-Flag immunoprecipitates (IP) were analyzed by immunoblotting for the indicated proteins and primer extension for the bound 7SK RNA."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "C-F; H-K; and N-Q. The tagged WT or mutant 7SK RNA was co-transfected with the indicated Flag-tagged proteins into HeLa cells. Anti-Flag immunoprecipitates (IP) were analyzed by immunoblotting for the indicated proteins and primer extension for the bound 7SK RNA."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "C-F; H-K; and N-Q. The tagged WT or mutant 7SK RNA was co-transfected with the indicated HA-tagged proteins into HeLa cells. Anti-HA immunoprecipitates (IP) were analyzed by immunoblotting for the indicated proteins and primer extension for the bound 7SK RNA."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "R. The indicated 7SK were in vitro transcribed in the presence of 32p-UTP and subjected to in vitro modification reactions. The nuclease P1-digested products were analyzed by 1D-TLC with pU and pΨ as controls."
NCBI gene IDs:
"7SK: 125050"
Figure legend: "A. Anti-V5immunoprecipitates (IP) from HeLa cells expressing nothing (-) or V5-DKC1 were analyzed by immunoblotting for V5-DKC1 and qRT-PCR for the bound 7SK RNA. The error bars represent mean ± SD from three independent experiments."
NCBI gene IDs:
"DKC1: 1736
7SK: 125050"
Figure legend: "B. Anti-FlagIP from NE of HeLa or the HeLa-derived F1C2 cells expressing CDK9-F were analyzed by Western blotting (WB) for the indicated proteins and primer extension (PEx) for 7SK RNA."
NCBI gene IDs:
"7SK: 125050"