EMBO/SourceData huggingface dataset:
Figure legend: "(B) FIP200 binds to exogenously expressed Atg16L1. Myc-Atg16L1 coprecipitations with an empty vector control (lane 1) or One-STrEP-FLAG (OSF)-FIP200 (lane 2)."
NCBI gene IDs:
"FIP200: 9821"
Figure legend: "(C) FIP200 binds endogenous Atg16L1. Atg16L1 coprecipitations with an empty vector control (lane 1) or OSF-FIP200 (lane 2)."
NCBI gene IDs:
"Atg16L1: 55054
FIP200: 9821"
Figure legend: "(D) Wild-type or FIP200-KO MEFs stably expressing the empty vector, full-length Atg16L1, or ΔWD mutant were incubated in growth medium (−) or Earle's balanced salt solution (EBSS) (+) for 1 h and examined by Western blotting using the indicated antibodies. We previously reported that stable expression of an exogenous Atg16L1 construct can be used to replace the endogenous Atg16L1 protein with an exogenous Atg16L1 protein (Fujita et al., 2009) because free Atg16L1 molecules not complexed with the Atg12-Atg5 conjugate are preferentially degraded by the ubiquitin-proteasome system. In control cells (Empty), two Atg16L1 splicing variants, the α- and β-forms, were detected. In full-length β-form-replaced cells (Full), the α-form was not detected, whereas in ΔWD-replaced cells (ΔWD) both the α- and β-forms were minimally detected. Thus, we successfully obtained cells that express only endogenous levels of the full-length or ΔWD mutant."
NCBI gene IDs:
"Atg16L1: 77040
FIP200: 12421"
Figure legend: "(E-L) Atg16L1-replaced wild-type or FIP200-KO MEFs were infected with Salmonella (MOI = 10) for 1 h and subjected to immunocytochemistry for Atg16L1 and Ub (E and F) or LC3 and Ub (I and J). The percentages of Atg16L1-positive (G and H) or LC3-positive per Ub-positive bacteria were enumerated (K and L). At least 50 Salmonella were counted. The average ± SD is shown for three independent experiments. Statistical analysis was performed by Student's t test. *, P &lt; 0.05; NS, not significant. Bar, 5 µm."
NCBI gene IDs:
"Atg16L1: 77040
FIP200: 12421"
Figure legend: "(E) The N-terminal 1-249 fragment of Atg16L1 is sufficient to interact with FIP200. Full-length (lanes 1 and 2) or the N-terminal 1-249 fragments (lanes 3 and 4) of Myc-Atg16L1 coprecipitations with empty vector controls (lanes 1 and 3) or OSF-FIP200 1276-1591 (lanes 2 and 4)."
NCBI gene IDs:
"Atg16L1: 55054
FIP200: 9821"
Figure legend: "(F) Co-precipitation experiments confirming that OSF-FIP200 1276-1591 coprecipitates with wild-type (WT) Myc-Atg16L1 (1-249) fragments, but not with analogous fragments containing a mutant FIP200-binding site (Myc-Atg16L1 ΔWD 239-242A)."
NCBI gene IDs:
"Atg16L1: 55054
FIP200: 9821"
Figure legend: "(B-E) Atg16L1-Δ/Δ MEFs stably expressing the indicated constructs were infected with Salmonella (MOI = 10) for 1 h and then analyzed by immunocytochemistry for LC3 (B) or Atg16L1 (D). Bar, 5 µm. The percentage of LC3- or Atg16L1-positive Salmonella per Ub-positive Salmonella was enumerated by fluorescence microscopy (C and E). At least 50 bacteria were counted. The average ± SD is shown for three independent experiments."
NCBI gene IDs:
"Atg16L1: 77040"
Figure legend: "(F) Atg16L1-Δ/Δ MEFs stably expressing the indicated constructs were cultured in growth medium (Fed) or EBSS (Starved) for 1 h and then subjected to immunocytochemistry using an anti-LC3 antibody. The number of LC3 puncta in each cell was counted for more than 50 cells. The average ± SD is shown for three independent experiments."
NCBI gene IDs:
"Atg16L1: 77040"
Figure legend: "(B-K') The Drosophila posterior midguts containing Su(H)-Gal4&gt;UAS-CD8:GFP (Su(H)&gt;GFP) with sucrose (Suc, control) (B-C'), P.e for 36 h (D-F'), Ecc15 for 36 h (G-I') and DSS for 36 h (J-K') were immunostained for GFP (green), PH3 (red) and DAPI (blue). (C-C', E-E', H-H' and K-K') Magnification of selected areas containing PH3+GFP─ cells from B, D, G and J. The EB cell close to the PH3+ cell (white arrow) in C-C' is out of focus. (F-F' and I-I') Magnification of selected areas containing PH3+ EBs (GFP+) from D and G. White arrowheads and red arrows indicate PH3 in GFP─ and GFP+ cells, respectively. (L and M) Quantification of PH3 GFP─ ISCs (L) and PH3+GFP+ EBs (M) with given treatments. n = 21 guts for each treatment."
NCBI gene IDs:
"GFP: 
CD8: 12526///12525
Gal4: 855828
Su(H): 34881"
Figure legend: "(D-F'') When the ISCs were marked by esgGal4, su(H)-Gal80&gt;UAS-GFP, PH3 expression was found in GFP+ ISCs with feeding of Suc (control) (D-D''), P.e (E-E'') and Dss (F-F''). However, PH3 expression is present in EBs with GFP─Pros─ and small nuclei upon P.e infection (E-E'', red arrows). The white arrows indicate GFP+ cells with PH3 expression. (G) Quantification of PH3+GFP─Pros─ EBs with given treatments. n = 11 guts for each treatment. Data information: Three independent experiments were performed, and error bars are ± SEM. ***, P &lt; 0.001 (Student's t-test) (G)."
NCBI gene IDs:
"GFP: 
esg: 34903
Gal4: 855828
Gal80: 854954
su(H): 34881"
Figure legend: "(D-N') The Drosophila midguts expressing Su(H)ts&gt;GFP with control (D-E'), UAS-RasV12 for 6 days (F-H'), EGFRA887T for 4 days (I-K') and UAS−λTop for 5 days (L-N') were immunostained for GFP (green), PH3 (red) and DAPI (blue). (E-E', G-G', J-J' and M-M') Magnification of selected areas containing PH3+GFP─ cells from D, F, I and L. (H-H', K-K' and N-N') Magnification of selected areas containing PH3+GFP+ cells from F, I and L. White arrowheads and red arrows indicate PH3 in GFP─ ISCs and GFP+ EBs, respectively. (O, P) Quantification of PH3+GFP─ (P) and PH3+GFP+ (O) in midguts with indicated genotypes. n = 13 guts for each genotype. (Q) Quantification of PH3+ EBs (GFP+) with the given genotypes in response to P. e infection. n = 11 guts for each genotype."
NCBI gene IDs:
"GFP: 
λTop: 39564
EGFR: 37455
Ras: 43873
Su(H): 34881"
Figure legend: "(B-C''') Flies expressing UAS-Flp; Su(H)ts UAS-CD8:GFP; ActP&gt;Stop&gt;LacZ were fed with Suc (B-B''') or P.e (C-C''') for 36h and their midguts were dissected and immunostained with antibodies against GFP, β-gal and Dl. Blue arrowheads indicate Dl+ ISCs; white arrows indicate GFP+ EBs; and red arrowheads indicate EB progeny cells (LacZ+GFP─). (D) Quantification of Dl+ ISC-like cells from EBs (GFP─LacZ+Dl+) in the control, P. e infected midguts, n=50. (E) Quantification of Dl+ ISC-like cells from EBs (GFP─LacZ+Dl+) in the control with Suc or P. e, or knockdown of EGFR with Suc or P. e infection, n=50."
NCBI gene IDs:
"Flp: 
GFP: 
LacZ: 
ActP: 31521
CD8: 12526///12525
EGFR: 37455
Stop: 252732
Su(H): 34881"
Figure legend: "(A-C''') The midguts from adult female flies expressing UAS-Flp; Su(H)ts UAS-CD8:GFP; ActP&gt;Stop&gt;LacZ with (B-B''') or without (A-A''') UAS-RasV12 or with UAS-RasV12 and UAS-stg-RNAi (C-C''') were dissected and immunostained with antibodies against GFP, β-gal and Dl. The bigger GFP─ nucleus with LacZ expression (only red arrowheads) indicate differentiating or mature ECs. Blue arrowheads indicate Dl+ ISCs; white arrows indicate GFP+ EBs; and red arrowheads indicate EB progeny cells (LacZ+GFP─). (D) Quantification of Dl+ ISC-like cells from EBs (GFP─LacZ+Dl+) in the indicated genotypes, n=50."
NCBI gene IDs:
"Flp: 
GFP: 
LacZ: 
ActP: 31521
CD8: 12526///12525
Ras: 43873
stg: 43466
Stop: 252732
Su(H): 34881"
Figure legend: "(C-E'') The representative twin clones from midguts when EGFRA887T is overexpressed in EBs (D-D'', red and green arrows) or adult flies were fed with P.e for 36h (E-E'', red and green arrows), but no twin clone is found in the control (C-C''). (F) Quantification of different types of twin clones in the control midguts, or in the midguts with EGFRA887T overexpression or P.e infection. n = 10 guts for each genotype."
NCBI gene IDs:
"EGFR: 37455"
Figure legend: "(A-C'') ISCs from EBs upon RasV12 expression or P. e infection could differentiate to generate EE or pre-EE cells (B-C'', LacZ+Pros+GFP─, blue arrowheads), but no EE or pre-EE cells from EBs are found in the control (A-A'', white arrowheads). (D) Quantification of EE cells from progenies of EBs with indicated genotypes or treatments. n = 9 guts for each genotype or treatment."
NCBI gene IDs:
"Ras: 43873"
Figure legend: "(A) Example regions exhibiting the IPOD-HR occupancy  where EPOD calls are made. Loose EPODs (pink) and Strict EPODs (teal) represent spans of high  protein occupancy at lower and higher thresholds, respectively. Neighboring areas showing lower robust z scores  are not EPODs. Note that following the definitions in [12], the presence of an EPOD requires a sustained region of high average occupancy, without any regions in which occupancy drops to background levels. Thus, breaks in EPOD calls represent cases where a rolling average occupancy drops below the calling threshold (e.g., the breakpoint in the middle of pinQ  in the top panel), and regions of apparent occupancy may nevertheless not be called due to being below the minimum length threshold (as is the case for nohA)."
NCBI gene IDs:
"nohA: 946021
pinQ: 946088"
Figure legend: "(E) Specific locations across all conditions remain occupied by protein where EPODs across a representative region, the waa operon, were called (left) and unoccupied by RNA polymerase (right). The quantile normalized robust z scores of the protein occupancy at each 5 bp are represented by the IPOD-HR occupancy."
NCBI gene IDs:
"waa: 948146///948145///948147///948151///948155///948150///948143///948148///948142///948140///948149///948135///948136///948144"
Figure legend: "(E) Protein occupancy  over the waa  operon. The quantile normalized  robust z scores of the  protein occupancy  at each 5 bp are represented by the  IPOD-HR occupancy. The EPOD over the waa  operon is lost in the ∆stpA∆hns  condition which results in increased accessibility and  RNA polymerase occupancy"
NCBI gene IDs:
"hns: 945829
stpA: 947130
waa: 949048///948144///948136///948135///948149///948140///948142///948148///948143///948150///948155///948151///948147///948145///948146
waa: 948146///948145///948147///948151///948155///948150///948143///948148///948142///948140///948149///948135///948136///948144///949048"
Figure legend: "(G) RNA-seq  analysis shows log fold change compared to WT of waa operon expression  upon deletion of hns  and stpA. (*) indicate q value &lt; 0.05 = *, &lt; 0.005 = **, &lt; 0.0005 = ***"
NCBI gene IDs:
"hns: 945829
stpA: 947130"
Figure legend: "(B) Protein occupancy over the idn  operon for each condition (colors are denoted in (A)). The quantile normalized robust z scores of the  protein occupancy at each 5 bp are represented by the  IPOD-HR occupancy. There is a large loss in protein occupancy when cells are shifted to 5KDG, leading to the loss of the call ed EPOD. Protein occupancy is restored once cells are grown in the second glucose  condition. The 500bp normalized average of previously published H-NS ChIP-seq [20] exhibits high  H-NS binding  on the idnD  promoter region."
NCBI gene IDs:
"idnD: 944769"
Figure legend: "(B) Difference in Log2 ratio of fitnesses of the KDG-exposed cells with unexposed lacZ::cat  relative to that observed prior to KDG  exposure, given as a function of time  since exposure to KDG. Day -1 corresponds to the day before KDG  exposure, Day 0 is cells taken immediately after growth  in KDG, and subsequent timepoints  reflect different durations of growth  in M9 Min Glu  prior to competition (See (A)). Points show medians across replicates (3 biological replicates for 0.5 days, 6 biological replicates for all other timepoints); error bars show 95% confidence intervals for the difference relative to the Day -1 timepoint, calculated using the R wilcox.test function. Significance was assessed using a Wilcoxon rank sum test comparing the distribution at each timepoint  to the -1 day (naive) timepoint: *, p&lt;0.05; **, p&lt;0.005."
NCBI gene IDs:
"cat: 
lacZ: 945006"
Figure legend: "(A) Example prophage  region that is annotated with the Fis- and Hfq-associated HMM class 5 in our genome-wide HMM classification The quantile normalized robust z scores of the  protein occupancy  at each 5 bp are represented by the  IPOD-HR occupancy. Prophage  genes are highlighted with a red box. The major peak associated with WT IPOD-HR occupancy is represented in a grey dashed line as a reference to compare  IPOD-HR occupancy in the other genotypes. Modest loss of  protein occupancy was observed at the same prophage-containing EPOD for ∆fis  (light purple; see color key in (B)) and ∆hfq  (dark purple; see color key in (B)) conditions compared to WT (gold)."
NCBI gene IDs:
"fis: 947697
Fis: 947697
hfq: 948689
Hfq: 948689"
Figure legend: "(B)  RNA-seq  of WT, ∆fis, and ∆hfq  were performed. The log fold change compared to WT was calculated at prophage  genes contained in the dashed box in (A). Induction of prophages  across the region where loss in occupancy is observed. (*) indicate the adjusted p-value: &lt;0.10 = +, &lt; 0.05 = *, &lt;0.005=**, &lt;0.0005=***"
NCBI gene IDs:
"fis: 947697
hfq: 948689"
Figure legend: "(F) Rate ratios of all genes (grey) and prophage  genes (red) in each quadrant outline in (E), showing a higher rate of genes that resided in the +/+ category, indicating that many prophages  are de-repressed in both ∆fis  and ∆hfq. (*) indicate the p-value calculated from testing the null that the rate ratios are the same. P-value &lt; 0.05 = *, &lt;0.005=**, &lt;0.0005=***"
NCBI gene IDs:
"fis: 947697
hfq: 948689"
Figure legend: "(G) P1 vir transduction  experiment to test the viability  of ∆fis  and ∆hfq. -Hfq  indicates deleting Hfq  and -CspE  indicates deleting -CspE  as a control. Strain identities are indicated in the box. Number of transductions  were counted on LB + Kan  plates; all efficiencies are relative to WT, and thus the log-scaled relative transduction rate for the WT itself is 0 by definition. -R1 indicates that the prophage region in (A) was deleted to test whether the loss of prophages silenced by Fis  and Hfq  restored viability  of a ∆fis  ∆hfq  genotype. R2 and R3 were other regions in the genome that contained prophages that appeared to have Fis/Hfq  dependent EPODs. Plotted values are mean efficiencies across replicates, with error bars showing a 95% credible interval obtained via Bayesian inference, assuming the replicate-level colony counts are Poisson distributed with a (conjugate) Gamma(0,0) prior; all log ratios (including error bounds) are plotted relative to the mean WT value. Data obtained from 5 biological replicates for hfq::kan  transductions  and 4 biological replicates for cspE::kan  transductions."
NCBI gene IDs:
"CspE: 947024
cspE: 947024
fis: 947697
Fis: 947697
hfq: 948689
Hfq: 948689"
Figure legend: "(C) Effects of deletion of rok  in the vicinity of an nEPOD; Rok ChIP  data from[70] shows a strong overlap with the nEPOD boundary, whereas that occupancy region is lost in Δrok cells."
NCBI gene IDs:
"rok: "
Figure legend: "(A, Neutrophils  isolated from tamoxifen-treated Nlrp3A350V/wt CreT+ and Nlrp3A350V/wt CreT- mice  were incubated with LPS  (3 hr) before culture media were analysed for secreted IL-1β (A) Cytokine values represent mean ± SEM of n=3 biological repeats. Statistical significance was analysed by two-way ANOVA and P-values were corrected for multiple comparisons (Bonferroni). **, p ≤ 0.01."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799"
Figure legend: "B). Neutrophils  isolated from tamoxifen-treated Nlrp3A350V/wt CreT+ and Nlrp3A350V/wt CreT- mice  were incubated with LPS  and cell lysates  and culture media were immunoblotted  for β-actin, caspase-1  and IL-1β (B)."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799"
Figure legend: "(C) Representative pictures of Nlrp3A350V/wt MRP8-CreTg and Nlrp3A350V/wt MRP8-Cre- mice  on days 4 and 8 after birth. Left side in each picture: Nlrp3A350V/wt MRP8-Cre- mice; right side in each picture: Nlrp3A350V/wt MRP8-CreTg. Abscesses  (day 4) and  scaling erythema (day 8) are observed in Nlrp3A350V/wt MRP8-CreTg mice."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799
MRP8: 20201"
Figure legend: "(D) Survival curves of  Nlrp3A350V/wt MRP8-CreTg (n=22) and littermate Nlrp3A350V/wt MRP8-Cre- (n=21) mice. Statistical significance was analysed by the log-rank Mantel-Cox test. ****, p ≤0.0001."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799
MRP8: 20201"
Figure legend: "(E) Representative H&amp;E-stained  sections of spleen  (top, scale bar: 100 µM) and liver  (bottom, scale bar: 200 µM) of Nlrp3A350V/wt MRP8-CreTg and littermate Nlrp3A350V/wt MRP8-Cre- mice. Spleens  of Nlrp3A350V/wt MRP8-Cre- control mice  (top left) display characteristic white pulp (WP) regions (example shown surrounded by dashed line) composed of  lymphoid follicles and periarteriolar lymphoid sheaths (PALS), which at this magnification are recognized by dark-purple staining, large aggregates of lymphocytes  surrounding the central artery (CA) and their distinctive separation from the red-staining red pulp (RP). WP  was largely absent in spleens of Nlrp3A350V/wt MRP8-CreTg (top middle). Megakaryocytes  (MK) are indicated for easier recognition of areas of extramedullary hematopoiesis (EMH). Most of the spleen parenchyma of Nlrp3A350V/wt MRP8-CreTg animals was replaced by EMH  of mostly myeloid  lineage, which can be recognized by the medium-sized cells with more cytoplasm, giving a lighter appearance (inset). In both the overview picture (top middle picture) and inset (top right) of Nlrp3A350V/wt MRP8-CreTg spleen, a small amount of EMH  of the erythroid  lineage is present that can be recognized by small aggregates of darker-stained, smaller cells (asterix,*). Liver  sections of both  Nlrp3A350V/wtMRP8-Cre- control mice  and diseased Nlrp3A350V/wt MRP8-CreTg pups  displayed comparable EMH  of the erythroid lineage (bottom left picture, indicated with yellow arrows). Liver  sections of Nlrp3A350V/wt MRP8-CreTg pups  also showed a modest increase in EMH  of the myeloid  lineage (bottom middle picture and inset (scale bar: 100 µM) in bottom right, indicated with yellow arrowheads), as well as hepatocyte  enlargement. (F-I) H&amp;E-stained histological  sections of spleen (F, G) or liver (H, I) of Nlrp3A350V/wt MRP8-Cre- (Cre-, n=8) and Nlrp3A350V/wt MRP8-CreTg (CreTg, n=6) mice  were assigned a score of 0-5 for lymphoid  depletion (F) or EMH (G, H) or hepatocyte  enlargement (I) by a board-certified pathologist. Values represent mean ± SEM of n=6-8 biological repeats. Statistical significance was analysed by the Mann-Whitney U-test. ***, p ≤0.001."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799
MRP8: 20201"
Figure legend: "(J-L) Cytokine secretion analysis  (Luminex) of serum  samples obtained at day 7 from Nlrp3A350V/wt MRP8-Cre- (Cre-, n=8) and Nlrp3A350V/wt MRP8-CreTg (CreTg, n=6) pups. Values represent mean ± SEM of n=6-8 biological repeats. Statistical analysis of serum cytokine levels  was performed by the Welch's t-test. *, p ≤0.05; **, p ≤0.01."
NCBI gene IDs:
"Cre: 2777477
Nlrp3: 216799
MRP8: 20201"
Figure legend: "(C) Levels of mucosal vtRNA2-1, vtRNA1-1, vtRNA1-2, and vtRNA1-3 in the colon of patients with active ulcerative colitis (UC). Values are the means ± SEM (n = 6 per group). Unpaired, two-tailed Student's t test was used. * P &lt; 0.05 compared with controls. (D) Levels of tissue vtRNAs in the ileal mucosa of patients with Crohn's disease (CD). Values are the means ± SEM (n = 6 per group). Unpaired, two-tailed Student's t test was used. * P &lt; 0.05 compared with controls."
NCBI gene IDs:
"vtRNA1-1: 56664
vtRNA1-2: 56663
vtRNA1-3: 56662
vtRNA2-1: 100126299"
Figure legend: "(A) Association of vtRNA2-1 with HuR in Caco-2 cells as measured by biotin-labeled RNA pull-down assays. After cytoplasmic lysates were incubated with biotinylated vtRNA2-1, levels of HuR and other proteins in the pull-down material were assessed. Three separate experiments showed similar results."
NCBI gene IDs:
"vtRNA2-1: 100126299"
Figure legend: "(D) Changes in the expression levels of claudin-1, occludin, and E-cadherin proteins 48 h after cells were transfected with HuR expression vector alone or co-transfected with HuR and vtRNA2-1 expression vectors."
NCBI gene IDs:
"HuR: 
vtRNA2-1: 100126299"
Figure legend: "Macrophages  were detected by immunoblotting  with indicated antibodies (n = 3). Data information: vehicles were PBS. For multiple groups, one-way ANOVA was used. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance. EPOR-KO macrophages  were PMs  isolated from EPOR-MKO mice."
NCBI gene IDs:
"EPOR: 13857"
Figure legend: "Flag-Aβ42  was injected into the  lateral ventricles of Lyz2-Cre+/+/Epor+/+ mice  (EPOR-C) (8 w) mice  pretreated with rhEPO  10000 IU/kg/d, i.p.) for 2 days (twice per day) (n = 3). One day later, Flag-Aβ42  in spleens  and brains  was detected by immunoblotting  (A, Data information: Unpaired t-tests were used for the comparison of two groups. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance."
NCBI gene IDs:
"Flag: 
Cre: 2777477
Epor: 13857
EPOR: 13857
Lyz2: 17105"
Figure legend: "Flag-Aβ42  was injected into the  lateral ventricles  of Lyz2-Cre+/+/Epor+/+ mice  (EPOR-C) (8 w) mice  pretreated with ARA290  (0.03 mg/kg/d, i.p.) for 2 days (twice per day) (n = 3). One day later, Flag-Aβ42  in spleens  and brains  was detected by immunoblotting  B, Data information: Unpaired t-tests were used for the comparison of two groups. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance. ARA, ARA290"
NCBI gene IDs:
"Flag: 
Cre: 2777477
Epor: 13857
EPOR: 13857
Lyz2: 17105"
Figure legend: "Flag-Aβ42  was injected into the  lateral ventricles of Lyz2-Cre+/+/Eporloxp/loxp mice  (EPOR-MKO) (8 w) mice  pretreated with rhEPO 10000 IU/kg/d, i.p.) for 2 days (twice per day) (n = 3). One day later, Flag-Aβ42  in spleens  and brains  was detected by immunoblotting  Data information: Unpaired t-tests were used for the comparison of two groups. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance."
NCBI gene IDs:
"Flag: 
Cre: 2777477
Epor: 13857
EPOR: 13857
Lyz2: 17105"
Figure legend: "Flag-Aβ42  was injected into the  lateral ventricles of Lyz2-Cre+/+/Eporloxp/loxp mice  (EPOR-MKO) (8 w) mice  pretreated with ARA290  (0.03 mg/kg/d, i.p.) for 2 days (twice per day) (n = 3). One day later, Flag-Aβ42  in spleens  and brains  was detected by immunoblotting  E). Data information: Unpaired t-tests were used for the comparison of two groups. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance. ARA, ARA290"
NCBI gene IDs:
"Flag: 
Cre: 2777477
Epor: 13857
EPOR: 13857
Lyz2: 17105"
Figure legend: "APP/PS1-21 mice  (5 m) treated with rhEPO (10000 IU/kg/d) or ARA290  (0.03 mg/kg/d) (i.p., once a day, for 14 days) (n=10), and Aβ plaques   in cortex  and hippocampus  (n = 5) were detected by IHC  (I). Data information: For multiple groups, one-way ANOVA was used. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance. ARA, ARA290; IHC, Immunohistochemical staining."
NCBI gene IDs:
"APP: 351
PS1: 5663"
Figure legend: "Proteins were extracted from spleens  of 5- or 11-month-old APP/PS-1-21+/+/Lyz2-Cre+/+/Eporloxp/loxp mice  (APP/PS1-21+EPOR-MKO) or APP/PS-1-21+/+/Lyz2-Cre+/+/Epor+/+ mice  (APP/PS1-21+EPOR-C), and soluble Aβ  levels were measured by ELISA  (n = 5) (J Data information: Unpaired t-tests were used for the comparison of two groups. Results are presented as mean ± s.d., *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001; ns denotes no statistical significance. ELISA, "
NCBI gene IDs:
"APP: 351
Cre: 2777477
Epor: 13857
EPOR: 13857
Lyz2: 17105
PS-1: 5663
PS1: 5663"