EMBO/SourceData huggingface dataset:
Figure legend: "One-cell stage embryos were injected animally with mRNA encoding Zic5 dominant-negative (0.5 ng) protein. Total RNA was isolated from five control embryos at neurula stage 18 (lane 1) and five embryos from the injected group (lanes 2). Neural AP markers were examined by sqRT-PCR: hindbrain (krox20) and spinal cord (cdx1-4). Ef1α is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 3)."
NCBI gene IDs:
"Ef1α: 
cdx1: 397695///108710769
krox20: 378520
Zic5: 108708473///373661"
Figure legend: "One-cell stage embryos were injected animally with the Zic1-MO (25 ng). Embryos underwent in situ hybridization. Expression of the panneural sox2 marker was normal, the forebrain marker xanf1 was expanded in 80% of the embryos, the hindbrain representative marker krox20 was reduced in 80% of the embryos and the representative spinal cord marker cdx4 was expanded in 90% of the embryos. N = 23 embryos were per marker."
NCBI gene IDs:
"Zic1: 399124"
Figure legend: "One-cell stage embryos were injected animally with mRNAs encoding the noggin (5/20 pg) and/or meis3 (0.5 ng) proteins. AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to neurula stage 17. Total RNA was isolated from seven control embryos (lane 2), and from eighteen ACs from each group (lanes 3-8). Neural AP markers were examined by sqRT-PCR: forebrain (xanf1, otx2), hindbrain (krox20, hoxb1, hoxb3), spinal cord (hoxb9, cdx1-4) and panneural (soxd). Ef1α is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"Ef1α: 
cdx1: 397695///108710769
krox20: 378520
xanf1: 397950
hoxb1: 100301962///108702519
hoxb3: 373719
hoxb9: 100381079///108700809
meis3: 379219///398093
noggin: 108704141///373646
otx2: 399342///432013
soxd: 397708"
Figure legend: "One-cell stage embryos were injected animally with mRNA encoding bmp4 (0.2 ng) protein. Total RNA was isolated from five control embryos at neurula stage 17 (lane 1) and five embryos from the injected group (lanes 2). Neural and mesodermal markers were examined by sqRT-PCR: forebrain (otx2), hindbrain (krox20, hoxb3), spinal cord (hoxb9, hoxc10, cdx4), panneural (nrp1, ncam) and mesodermal (muscle actin - MA). ODC is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"ODC: 
bmp4: 397874///399322
cdx4: 108700082///380412
krox20: 378520
hoxb3: 373719
hoxb9: 100381079///108700809
hoxc10: 399206
MA: 108706299
muscle actin: 108706299
ncam: 397761///397762
nrp1: 397804
otx2: 399342///432013"
Figure legend: "One-cell stage embryos were injected animally with mRNA encoding bmp4 (0.2 ng) protein Embryos underwent in situ hybridization. Expression of the hindbrain marker hoxb3 was reduced/eliminated by bmp4 (0.2 ng) in 68% of the embryos (n=19). Expression of the spinal cord marker cdx4 was slightly increased/normal, in 100% of the embryos (n=19)."
NCBI gene IDs:
"bmp4: 397874///399322"
Figure legend: "One-cell stage embryos were injected animally with increasing concentrations of bmp4 encoding mRNA (5-50 pg). AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to neurula stage 16. Total RNA was isolated from five control embryos (lane 2), and from eighteen ACs from each group (lanes 3-7). Neural, epidermal and mesodermal markers were examined by sqRT-PCR: hindbrain (krox20), spinal cord (hoxb9, hoxc10/d10, cdx1-2-4), epidermal cytokeratin (E13), lateral mesoderm (osr1-2) and dorsal-lateral mesoderm muscle actin (MA). ODC is the control for quantitating the RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"ODC: 
bmp4: 397874///399322
cdx1: 397695///108710769
krox20: 378520
hoxb9: 108700809///100381079
hoxc10: 399206
d10: 399032
cytokeratin (E13): 379143
MA: 108706299
muscle actin: 108706299
osr1: 100313967"
Figure legend: "One-cell stage embryos were injected animally with mRNAs encoding the bmp4 (50/150 pg) and/or meis3 (0.7 ng) proteins. AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to neurula stage 16. Total RNA was isolated from five control embryos (lane 2), and from eighteen ACs from each group (lanes 3-8). Neural and mesodermal markers were examined by sqRT-PCR: hindbrain (krox20), spinal cord (hoxb9, hoxc10, cdx2), and lateral mesoderm (osr1). Ef1α is the control for quantitating the RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"Ef1α: 
bmp4: 397874///399322
cdx2: 735025///397753
krox20: 378520
hoxb9: 108700809///100381079
hoxc10: 399206
meis3: 379219///398093
osr1: 100313967"
Figure legend: "One-cell stage embryos were injected animally with increasing concentrations of bmp4 encoding mRNA (75-200 pg). AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to gastrula stage 11-11.5. Total RNA was isolated from five control embryos (lane 2), and from eighteen ACs from each group (lanes 3-6). Marker expression was determined by sqRT-PCR. Xenopus brachyury (xbra) expression was induced by BMP4 in a dose-dependent manner. Marker expression was determined by sqRT-PCR. Neither dorsal (chd, goosecoid-gsc) or ventral-lateral (osr1) mesoderm markers were induced by BMP4. Sox2/3 neural markers are expressed in the ACs and are unchanged by BMP expression. EF1α is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"EF1α: 
bmp4: 397874///399322
BMP4: 397874///399322
BMP: 399322
chd: 733207
goosecoid: 397748///397752
gsc: 397748///397752
osr1: 100313967
Sox2: 398000
Xenopus brachyury: 399275
xbra: 399275"
Figure legend: "One-cell stage embryos were injected animally with increasing concentrations of bmp4 encoding mRNA (5-50 pg). AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to neurula stage 16. Total RNA was isolated from five control embryos (lane 2), and from eighteen ACs from each group (lanes 3-6). FGF3-4-8 expression was determined by sqRT-PCR. ODC is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"bmp4: 
ODC: 
FGF3: 108715251///373669"
Figure legend: "One-cell stage embryos were injected animally with mRNAs encoding the bmp4 (50 pg) and/or FGF dominant-negative receptor (FGF-DNR, 1.5 ng) proteins. AC explants were removed from control and injected embryos at blastula stage 9, and explants were cultured to neurula stage 16. Total RNA was isolated from five control embryos (lane 2), and from eighteen ACs from each group (lanes 3-6). In addition to fgf3, neural, epidermal and mesodermal markers were examined by sqRT-PCR: hindbrain (krox20, hoxb3), spinal cord (hoxb9, hoxc10, cdx4), lateral mesoderm (osr1), dorsal-lateral mesoderm muscle actin (MA), and epidermal cytokeratin (E13). ODC is the control for quantitating RNA levels. -RT-PCR was performed on total RNA isolated from control embryos (lane 1)."
NCBI gene IDs:
"bmp4: 
ODC: 
cdx4: 108700082///380412
krox20: 378520
fgf3: 108715251///373669
FGF: 394418
hoxb3: 373719
hoxb9: 100381079///108700809
hoxc10: 399206
cytokeratin (E13): 379143
MA: 108706299
muscle actin: 108706299
osr1: 100313967"
Figure legend: "Representative BLI traces showing decreased binding of Nxph1 to the Nrxn1 LNS2SS2- (I401Q) mutant compared to that of wild-type Nrxn1 LNS2SS2-. Replicate numbers are shown in E."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "Representative BLI traces showing increased binding of Nxph1 to Nrxn1 LNS2SS2A+ and Nrxn1 LNS2SS2AB+ compared to that of Nrxn1 LNS2SS2-. Replicate numbers are shown in E."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "Comparison of binding affinities for Nxph1 binding to mouse Nrxn1 LNS2 splice variants and splice-insert containing Nrxn1 LNS2 with the I401Q mutation; error bars represent SEM and replicate numbers are indicated in or above bars. Significance values were calculated using Welch's t-test, *P &lt; 0.05, **P &lt; 0.01, ****P &lt; 0.0001."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "Comparison of binding affinities of Nxph1 for Nrxn1 LNS2SS2- and Nrxn1 LNS2SS2A+ with or without Ca2+ added; replicate numbers are indicated in bars. Error bars represent SEM, and significance values were calculated using Welch's t-test. ns, not significant."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "C. Representative images of calyx synapses from littermate control and Nrxn123 TKO mice. Brainstem sections were labeled with antibodies to vGluT1 (red) and Syt2 (green). Scale bar, 10 μm. D. Summary graphs of the synaptic vGluT1 and Syt2 immunostaining intensity (normalized to control). "
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "E-H. Representative traces of spontaneous EPSCs (sEPSCs)(E), and summary graphs of the sEPSC frequency (F), amplitude (G), and kinetics (H) recorded from littermate control and Nrxn123 TKO mice."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "A. Representative traces of EPSCs evoked by paired stimuli separated by 10 ms and repeated every 20 s, recorded in a standard bath solution containing 2 mM Ca2+. B. Pan-neurexin deletion suppresses synaptic transmission. Summary graphs show the amplitude (left) and charge transfer (right) of the first EPSC recorded in response to the paired stimuli. C. Pan-neurexin deletion more than doubles the paired-pulse ratio (PPR), suggesting a large decrease in release probability. D. Pan-neurexin deletion decelerates the EPSC time course. Summary graphs show the rise time (left) and decay time constants (right) of the first EPSC in response to the paired stimuli. "
NCBI gene IDs:
"neurexin: 18191///18190///18189"
Figure legend: "E-F. Pan-neurexin deletion does not significantly alter the cumulative EPSC amplitude during a high-frequency stimulus train (100 Hz for 0.5 sec). Left, representative ESPC traces; right, cumulative summary plot of the EPSC amplitudes during the train (dotted lines show linear regression fits for estimating the cumulative EPSC amplitude by back-extrapolation to zero time, which is used to correct for vesicle replenishment during the train)."
NCBI gene IDs:
"neurexin: 18191///18190///18189"
Figure legend: "G. Pan-neurexin deletion does not decrease the readily-releasable pool of vesicles, but lowers the initial release probability during a high-frequency stimulus train. Summary graphs show the cumulative EPSC amplitudes extrapolated to time zero as an estimate of the readily-releasable pool size (left), and the ratio of the first EPSC amplitude divided by the cumulative EPSC amplitudes extrapolated to time zero as an estimate of the initial release probability (right)."
NCBI gene IDs:
"neurexin: 18191///18190///18189"
Figure legend: "F. Summary graph of the relative contribution of P/Q-type Ca2+ channels to the total presynaptic Ca2+ currents, as quantified by the relative reduction in Ca2+ currents evoked by step depolarization to +10 mV, in control and Nrxn123 TKO synapses."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "A. Representative traces of Ca2+-currents and 4-AP insensitive K+-currents evoked by step depolarizations (from −50 mV to +10 mV in 10 mV increments), recorded from the calyx terminals in acute slices from littermate control and Nrxn123 TKO mice at P12-P14. B. Same as (A) but after addition of 200 nM iberiotoxin (IbTx) by perfusion. C. Representative traces of presynaptic BK currents , which are calculated by subtracting currents shown in (B) from currents shown in (A). D. Summary graphs of the capacitance, Ca2+-current density, and BK-current density. "
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "A. Representative images of brainstem cryosections stained by triple immunofluorescence labeling for vGluT1 (green), CaV2.1-type Ca2+-channels (red), and the active zone protein Bassoon (purple). Cryosections were obtained from littermate control and Nrxn123 TKO mice at P12-14. Scale bar, 10 μm."
NCBI gene IDs:
"Nrxn1: 18189"
Figure legend: "(B, C) Body weight of the WT and L-HRD KO mice at the age of 12 weeks. (n=5 for each group) Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD: 74126"
Figure legend: "(D) Body heights and tibia lengths of the WT and L-HRD KO mice. (n=5 for each group) Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD: 74126"
Figure legend: "(E) Phosphorylation of STAT5 in WT and HRD1 LKO liver."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(E) Hepatic growth hormone signaling target genes mRNA levels in the WT and L-HRD1 KO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.)."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "Hepatic growth hormone signaling target genes mRNA levels in the WT and L-HRD1 KO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(A) Volcano plot of differential genes between WT and HRD1 LKO livers."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(B) Hepatic Fgf21 mRNA in the WT and L-HRD1 KO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"Fgf21: 56636
HRD1: 74126"
Figure legend: "(C) Serum FGF21 protein levels in the WT and L-HRD1 KO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(D) Correlation analysis of the gene expression (Red: Upregulated genes and Blue: Downrelated genes) between HRD1 LKO mice and FGF21 transgenic mice."
NCBI gene IDs:
"FGF21: 56636
HRD1: 74126"
Figure legend: "(E) Percentage of mated with proven stud males and age at onset of puberty in the female WT and L-HRD KO mice. (n=10 for each group) Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD: 74126"
Figure legend: "(F) Examples of ovarian histology from WT and HRD1 LKO mice."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(G) Representative examples of estrus cycles in WT and L-HRD1 KO mice as determined by vaginal cytology."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(I) H&amp;E stain of white adipose tissue WT and HRD1 LKO mice 14 weeks after HFD feeding."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(J) Ucp1 and Dio2 mRNA levels of adipose tissue from (I) (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"Dio2: 13371
Ucp1: 22227"
Figure legend: "(B) Volcano plot of differential proteins between WT and HRD1 LKO livers."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(F) Protein levels of IRE1α, NRF1, RXRβ, NOTCH1, SIRT3 and GAPDH in the liver of WT and HRD1 LKO mice under the refed condition. (n=6 for each group)."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "Hepatic CREBH protein levels in the WT and L-HRD1 KO mice. (n=6 for each group)."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "Hepatic CREBH mRNA (B) levels in the WT and L-HRD1 KO mice. (n=6 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.)."
NCBI gene IDs:
"CREBH: 208677
HRD1: 74126"
Figure legend: "(C) Western Blot analysis CREBH protein stability after HRD1 over-expression. Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD1: "
Figure legend: "(D) Western Blot analysis of hepatic CREBH and HRD1 protein levels in WT and HRD1 LKO mice. The mice were fasted 16 hours and refed for another 4 hours."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(H) Hepatic CREBH ubiquitination level in the WT and L-HRD1 KO mice"
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(J) Primary hepatocytes were isolated from WT and HRD1 LKO mice and Western Blot analysis CREBH protein stability."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(C, D) Ubiquitination of CREBH and CREBH K294R were measured after co-transfected C-terminal of HRD1."
NCBI gene IDs:
"HRD1: 
CREBH: 84699"
Figure legend: "(E) Western Blot analysis CREBH or CREBH-K294R protein stability after HRD1 protein over-expression. Data information: The data are representative of three independent experiments (mean ± s.d."
NCBI gene IDs:
"CREBH: 84699"
Figure legend: "(F) Western Blot analysis of the CREBH WT or K48 only ubiquitination level after HRD1 C-terminal co-expression."
NCBI gene IDs:
"HRD1: 
CREBH: 84699"
Figure legend: "(H) Western Blot analysis of the CREBH K6, K11, K27, K29 and K33 only ubiquitination level after HRD1 co-expression."
NCBI gene IDs:
"HRD1: 
CREBH: 84699"
Figure legend: "(I) Western Blot analysis of the CREBH WT, K27R ubiquitination level after HRD1 full-length co-expression."
NCBI gene IDs:
"HRD1: 
CREBH: 84699"
Figure legend: "(J) Western Blot analysis of the CREBH WT, K27 and K48 only ubiquitination level after HRD1 C-terminal co-expression."
NCBI gene IDs:
"HRD1: 
CREBH: 84699"
Figure legend: "(C) Hepatic Fgf21 mRNA in the WT and L-HRD1 KO mice 5 weeks after AAV-shCrebh injection. (n=5 for each group)."
NCBI gene IDs:
"Fgf21: 56636
HRD1: 74126"
Figure legend: "(D) Serum FGF21 protein levels in the WT and L-HRD1 KO mice 5 weeks after AAV-shCrebh injection. (n=5 for each group)."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(E) Body height and tibia length of the WT and L-HRD1 KO mice 5 weeks after AAV-shCrebh injection (n=5 for each group)."
NCBI gene IDs:
"HRD1: 74126"
Figure legend: "(F) Hepatic Igf1, Cyp2d9 and Cyp4a12 mRNA in the WT and L-HRD1 KO mice 5 weeks after AAV-shCrebh injection. (n=5 for each group)."
NCBI gene IDs:
"Cyp2d9: 13105
Cyp4a12: 277753///13118
Igf1: 16000
HRD1: 74126"
Figure legend: "(G, H) Percentage of the mated and estrus cycle of the WT and L-HRD KO 5 weeks after AAV-shCrebh injection. Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"HRD: 74126"
Figure legend: "(A) Examples of the body sizes of the WT, L-HRD KO and HRD1/FGF21 DKO mice. (B, C) Body weight and body height of the WT, L-HRD KO and HRD1/FGF21 DKO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"FGF21: 56636
HRD: 74126
HRD1: 74126"
Figure legend: "(D) Tibia lengths of the WT, L-HRD KO and HRD1/FGF21 DKO mice fed with normal chow diet. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"FGF21: 56636
HRD: 74126
HRD1: 74126"
Figure legend: "(E) Hepatic Hsd3b5, Cyp2d9 and Cyp4a12 mRNA in the WT, L-HRD1 KO and HRD1/FGF21 DKO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"Cyp2d9: 13105
Cyp4a12: 277753///13118
FGF21: 56636
Hsd3b5: 15496
HRD1: 74126"
Figure legend: "(F) Estrus cycle of L-HRD KO and HRD1/FGF21 DKO mice at the age of 4 months. Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"FGF21: 56636
HRD: 74126
HRD1: 74126"
Figure legend: "(G) Percentage of the Activity, feeding and water intake of the WT, L-HRD KO and HRD1/FGF21 DKO mice. (n=5 for each group). Data information: The data are representative of three independent experiments (mean ± s.d.). *: P&lt;0.05. **: P&lt;0.01 by unpaired student's t test."
NCBI gene IDs:
"FGF21: 56636
HRD: 74126
HRD1: 74126"
Figure legend: "(D,E) Pharmacological inhibition (for example, 3‐methyladenine (3‐MA), 10 mM) or genetic deletion (for example, ATG5−/−) of the autophagy pathway decreases the protein and mRNA expression of UVRAG and increases apoptosis at 12 h in HL60 cells treated with doxorubicin (Doxo) and UV irradiation (n=3, *P0.05). AU, arbitrary units; C‐PARP, cleaved poly‐ADP ribose polymerase; LC3, light chain 3; mRNA, messenger RNA; PI, propidium iodide; UV, ultraviolet; UVRAG, ultraviolet irradiation resistance‐associated gene."
NCBI gene IDs:
"ATG5: 11793"
Figure legend: "(E) After transfection with UVRAG shRNA or control shRNA for 48 h, ATG5+/+ and ATG5−/− murine embryonic fibroblasts (MEFs) were treated with doxorubicin (1 μg/ml), with or without Z‐VAD‐FMK (20 μM) for 12 h, and then apoptosis was assayed (n=3, *P0.05)."
NCBI gene IDs:
"ATG5: 11793
UVRAG: 78610"
Figure legend: "(F) Nude mice were inoculated with 4 × 106 HL60 tumour cells following transfection of control (shControl) or UVRAG (shUVRAG)‐specific shRNA and treated with doxorubicin (10 mg/kg) beginning at day 7. Tumours were measured twice weekly, and volumes were calculated for 21 days (n=10, *P0.05; shControl+Doxo compared with shUVRAG+Doxo)."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(A) Control shRNA or UVRAG shRNA cells were transfected with BAX plasmid, and apoptosis was assayed (n=3, *P0.05)."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(C) After transfection with UVRAG vector or control vector for 48 h, cells were transected or treated with BAX (1 μg), BAD (1 μg), BID (1 μg), FAS antibody (10 μg/ml) or TRAIL (100 ng/ml) for 24 h, then cell apoptosis was analysed (n=3, *P0.05)."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(D) Knockdown of UVRAG in BAX−/− HCT116 cells restored the sensitivity to UV treatment‐induced apoptosis (n=3, *P0.05). AU, arbitrary units; BAX, BCL2‐associated X protein; shRNA, short‐hairpin RNA; UV, ultraviolet; UVRAG, ultraviolet irradiation resistance associated gene."
NCBI gene IDs:
"BAX: 581
UVRAG: 7405"
Figure legend: "(D) UVRAG C2 domain deletion terminates BAX binding. HCT116 cells were transfected with Flag‐UVRAG or its mutants (ΔC2, ΔCCD), and then assayed for protein expression levels as indicated by IP or western blot."
NCBI gene IDs:
"UVRAG: 7405"
Figure legend: "(E) Apoptosis was assayed in indicated HCT116 cells after treatment with doxorubicin (Doxo, 1 μg/ml), UV irradiation (5 min after 50 mJ/cm2) or transfected with BAX (1 μg) for 24 h. BAX, BCL2‐associated X protein; CCD, coiled‐coil domain; CHAPS, 3‐[(3‐cholamidopropyl)‐dimethylammonio]‐1‐propane sulphonate; GST, glutathione‐S‐transferase; IB, immunoblotting; IgG, immunoglobulin G; IP, immunoprecipitation; RIPA, radioimmunoprecipitation assay; SDS-PAGE, SDS-polyacrylamide gel electrophoresis; UV, ultraviolet; UVRAG, ultraviolet irradiation resistance associated gene."
NCBI gene IDs:
"BAX: 581
UVRAG: 7405"
Figure legend: " UVRAG inhibits mitochondrial translocation of BAX. After transfection with indicated shRNA or cDNA for 48 h, HL60 cells were treated with doxorubicin (Doxo) and UV irradiation for 12 h, and then BAX in cytosol (Cyt) and mitochondria (Mit) was assayed by western blotting (A-C) and activation of BAX (D-F) was assayed by western blotting after IP using BAX monoclonal antibody (clone 6A7) *P0.05. AU, arbitrary units; BAX, BCL2‐associated X protein; CHAPS, 3‐[(3‐cholamidopropyl)‐dimethylammonio]‐1‐propane sulphonate buffer; Cyt c, cytochrome c; IP, immunoprecipitation; shRNA, short‐hairpin RNA; UV, ultraviolet; UVRAG, ultraviolet irradiation resistance associated gene. "
NCBI gene IDs:
"UVRAG: 7405"