EMBO/SourceData huggingface dataset:
Figure legend: "A HeLa cells were transfected with nontargeting siRNA (siCtr) or siRNAs silencing Rabaptin5 (siRbpt5) or FIP200 (siFIP200) for 72 h. The cells were infected with Salmonella by centrifugation at 500×g for 5min at 37°C and incubation for 10 min at 37°C, washed three times, and incubated in fresh culture medium containing gentamicin to prevent growth of extracellular bacteria for 0, 1, 3, or 6 h before lysis of the host cells and plating of the bacteria on LB agar plates at various dilutions to determine the number of live bacteria at the different time points, shown as a percentage of internalized cells after infection (mean ± S.D. of three independent experiments). The fractions of internalized bacteria alive 1 h after infection are shown separately in the middle (mean ± S.D. of three independent experiments; ANOVA: *P&lt;0.05). On the right, the fraction of infected cells was determined for HeLa cells transfected with siRNAs and infected as above with Salmonella expressing GFP, washed, and immediately fixed for fluorescence microscopy and stained with anti-transferrin receptor and anti-LC3B as cellular markers. Z-stacks for &gt;5'000cells/sample were acquired and analyzed in Fiji to determine the fraction of infected cells (mean ± S.D. of three independent experiments). The average number of bacteria per infected cell was identical (2.16, 2.11, and 2.12 bacteria per cell transfected with siCtr, siRbpt5, and siFIP200, respectively)."
NCBI gene IDs:
"Rabaptin5: 9135
Rbpt5: 9135
FIP200: 9821"
Figure legend: "B Wild-type HEK293A, HEK+Rbpt5, Rbpt5-KO, Rbpt5-KO+wt, Rbpt5-KO+AAA, and FIP200-KO cells were infected with Salmonella and treated and analyzed as in panels A (mean ± S.D. of three independent experiments; ANOVA: *P&lt;0.05, ****P&lt;0.0001)."
NCBI gene IDs:
"Rbpt5: 9135
FIP200: 9821"
Figure legend: "C Wild-type HEK293A, HEK+Rbpt5, Rbpt5-KO, Rbpt5-KO+AAA, and FIP200-KO cells were infected with Salmonella expressing GFP as in panel B, incubated in fresh culture medium containing gentamicin for 0, 5, 15, 30, and 60 min, fixed with methanol and immunostained for transferrin receptor (TfR) as a marker of early endosomes and for LC3B as a marker of autophagy. Salmonella were classified according to their association with a TfR- and/or LC3B-positive compartment - as illustrated on the top left (scale bar, 2 µm) - during the first hour after infection. In the absence of Rabaptin5, LC3-positive SCVs with early endosomal characteristics (containing TfR) were strongly reduced. (Mean ± S.D. of three independent experiments, analyzing &gt;50 bacteria for each time point.)"
NCBI gene IDs:
"Rabaptin5: 9135
Rbpt5: 9135
FIP200: 9821"
Figure legend: "(D-F) Quantification of the Rango-3 E gradients (top panels) and average cellular Rango-3 E (bottom) in involving untreated oocytes (controls) or oocytes injected with RanT24N mRNA, importin β(71-876) protein or treated with 20 µM IPZ. Data for each panel are from at least 2 separate experiments. Means ± SDs, t-test, oocyte numbers indicated in brackets."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(A) Quantification of chromosomal cargo gradients (top) and average Rango-3 E in oocytes injected with increasing concentrations of Ran mutant mRNA concentrations. Data from oocytes imaged side-by-side within one experiment. Means ± SDs, one-side ANOVA, Tukey's test. Numbers of analyzed oocytes are indicated in the brackets in the top panel and are identical for both panels."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(D) Quantification of the Rango-3 E gradients (top panels) and average cellular Rango-3 E (bottom) oocytes that were treated side-by-side by the injection of mRNA for Rango-3, alongside with buffer (controls) or 2000ng/µl mRNA for RanT24N or RanT24N, T42A. Means ± SDs, one-side ANOVA, Tukey's test, oocyte numbers indicated in brackets."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(D-F) Quantification of the RBP-3 gradients (top panels) and average cellular RBP-3 E (bottom) in separate experiments involving untreated oocytes (controls) or oocytes injected with RanT24N mRNA, importin β(71-876) protein or treated with 20µM IPZ. Means ± SDs, t-test, oocyte numbers indicated in brackets."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(A) Dose-dependent effects of IPZ on MI spindle assembly are shown by maximum z-projection images from confocal time-lapse imaging of oocytes obtained from transgenic histone H2B-EGFP mice stained with SiR-tubulin. Dashed circles indicate oocyte edges; scale bars, 10 µm."
NCBI gene IDs:
"EGFP: 
histone H2B: "
Figure legend: "(E) MI spindle formation in oocytes expressing CDK5RAP2-EGFP, histone H2B-mCherry and stained with SiR-tubulin matured in the presence of 15 µM IPZ. Representative still images of maximum z-projections are shown. Time in hh:mm is relative to GVBD. Scale bar, 10 µm."
NCBI gene IDs:
"EGFP: 
histone H2B: 
mCherry: 
CDK5RAP2: 214444"
Figure legend: "(H) Oocytes expressing CDK5RAP2-EGFP, histone H2B-mCherry and stained with SiR-tubulin were treated with 15 µM IPZ after being cultured for 5h in control media. Maximum intensity z-projections from confocal live-cell imaging are shown. Time in hh:mm is relative to GVBD, DMSO or 15 uM IPZ was added at time 5:00. Scale bars, 10 µm."
NCBI gene IDs:
"EGFP: 
histone H2B: 
mCherry: 
CDK5RAP2: 214444"
Figure legend: "(A) The rescue of the effects of 15 µM IPZ on MI spindles was examined in mouse oocytes expressing histone H2B-mCherry from microinjected mRNA, co-stained with SiR-tubulin and noninjected or injected with mRNAs coding for RanQ69L or hTPX2. Maximum intensity z-stack projections from confocal live-cell movies 30 min after GVBD, at metaphase I and anaphase I. Time in hh: mm is relative to GVBD. Scale bars, 10 µm."
NCBI gene IDs:
"histone H2B: 
mCherry: 
Ran: 5901
hTPX2: 22974"
Figure legend: "(A) The effects of RanT24N, T42A, and RanT24N on MI spindle assembly observed in maximum z-projection images from confocal time-lapse imaging of oocytes expressing CDK5RAP2-EGFP, histone H2B-mCherry and stained with SiR-tubulin. Time in hh:mm is relative to GVBD. Scale bar, 10 µm. (B, C) Live-cell imaging of oocytes as in (A) was used to quantify the effects of Ran mutant. Means ± SDs., Mann-Whitney test, oocyte numbers are indicated in brackets. "
NCBI gene IDs:
"EGFP: 
histone H2B: 
mCherry: 
CDK5RAP2: 214444
Ran: 5901"
Figure legend: "(D) MT intensity at MTOCs at the time of GVBD quantified from SiR-tubulin signal that was normalized to mean cytoplasmic SiR-tubulin signal. Mean of MT intensity on MTOCs was arbitrarily centered to 1 in the control group. Means ± SDs, Kruskal-Wallis, and Dunn's tests. 25 control oocytes with 264 MTOCs in total, 10 RanT24N-expression oocytes with 70 MTOCs in total and 19 RanT24N, T42A-expressing oocytes with 96 MTOCs in total were analyzed."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(F) Frequency and severity of MI spindle phenotypes in the oocytes expressing RanT24N or RanT24N, T42A. Oocyte numbers are shown in brackets."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(I) Detection of MTs and pericentrin-containing MTOCs by IF in control and RanT24N, T42A - expressing oocytes. Arrow indicates unaligned chromosome. (J) Quantification of chromosomal spread area in oocytes depicted in (I), Means ± SDs, Mann-Whitney test, oocyte numbers indicated in brackets. "
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(A). Maximum intensity z-stack projections of confocal IF images (DNA stain, indirect IF for tubulin and TPX2) in untreated oocytes (control), oocytes expressing RanT24N or injected with importin β(71-876). The representative of at least 5 experiments is shown."
NCBI gene IDs:
"Ran: 5901"
Figure legend: "(E) Western blot analysis with representative blot including NLRP3, caspase 1, IL-1β and actin levels in heart and liver tissues from wild-type and Zmpste24-/- mice."
NCBI gene IDs:
"Zmpste24: 230709"
Figure legend: "(F) NLRP3 and Caspase 1 transcript expression levels were determined in heart and liver tissues by real-time quantitative RT-PCR. n=5 for Zmpste24+/+ and n=5 for Zmpste24-/- groups respectively. ***P &lt; 0.001, **P &lt; 0.005, wild-type vs Zmpste24-/- mice. Data are showed means ± SD, n = 4 mice per group."
NCBI gene IDs:
"Caspase 1: 12362
NLRP3: 216799
Zmpste24: 230709"
Figure legend: "(F) Kaplan-Meier graph showing a significant increase in the maximum lifespan in WT mice compared with Zmpste24-/- mice. N=7 per group."
NCBI gene IDs:
"Zmpste24: 230709"
Figure legend: "(H) Analysis of serum concentrations of IL-1β measured by ELISA. N=6 per group. Data are shown as means ± SD. ***P &lt; 0.001, wild-type vs Zmpste24-/- mice; aaaP &lt; 0.001; vehicle vs MCC950."
NCBI gene IDs:
"Zmpste24: 230709"
Figure legend: "Top, average colon  length of WT and QSOX1  KO mice  following indicated treatments. For untreated mice, colon  lengths were calculated for 3 WT and 3 KO. For treated mice, lengths were calculated from 5 mice  except for the DSS-treated WT, for which 3 mice  were analyzed. Error bars are standard deviation. Statistical analysis was performed using Tukey multiple comparison of means (** p&lt;0.01). Administration of QSOX1  inhibitory antibody MAb316.1 (αQSOX1), did not enhance the sensitivity of WT colons  to DSS  (p&gt;0.4 for MAb316.1-treated WT vs. WT control antibody (IgG)-treated; p&gt;0.5 for MAb316.1-treated WT vs. WT without antibody treatment). Bottom, representative images of colons following DSS  treatment. Scale bar is 1 cm."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "Representative TEM  micrographs of about a hundred collected from 3 mice  of each genotype show large and well-packed goblet cell granules   in WT and QSOX1  KO colons. Scale bar is 5 µm."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "Alcian blue and PAS staining  of reduced guanidine-insoluble mucins  from WT and QSOX1  KO mice, separated on a 6% polyacrylamide  gel. Data information: each lane corresponds to a sample from one mouse."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "Muc5b  fluorescent immunoblot  of WT and QSOX1  KO lung  lavage samples separated on agarose  gels. Data information: each lane corresponds to a sample from one mouse."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "Western blot  analysis of blood Vwf  from WT and QSOX1  KO mice  separated on agarose  gels. Data information: each lane corresponds to a sample from one mouse."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "QSOX1 immunofluorescence  (green) in control (siControl) and QSOX1  knockdown (siQSOX1) MDA-MB-231  cells. Blue is DAPI staining  of nuclei. Scale bar is 20 µm. Data information: The results described in are representative of 3 experiments that were performed."
NCBI gene IDs:
"QSOX1: 5768"
Figure legend: "Western blot  analysis of the MUC2  N-terminal region and indicated cysteine mutants in supernatants of transfected cell cultures. No differences in disulfide-mediated dimerization  were observed for any MUC2  variant between siC (siControl) and siQ (siQSOX1). Data information: The results described in G are representative of 3 experiments that were performed."
NCBI gene IDs:
"MUC2: 4583
QSOX1: 5768"
Figure legend: "Western blot analysis of colon epithelial cell  lysates from WT and QSOX1  KO mice. Lysates were treated with   PEG-mal  2  kDa or NEM  as indicated above each blot  and probed using antibodies to St6gal1. As for panel A but using antibodies to St3gal1. As for panel A but using antibodies to B3galt5. Data information: For the experiments in panels A-C, equal amounts of total protein were applied to each gel lane, and no reducing agent was added. Experiments were repeated 3 times with fresh new lysates, and representative blots  are shown."
NCBI gene IDs:
"QSOX1: 104009"
Figure legend: "E WT and C2βD1212A/D1212A mice were subjected to  thromboembolic stroke model. Representatives T2-weighted MRI of coronal section taken 24 hours after the onset of in situ clot formation by α-thrombin were shown. Graph quantification of infarct volume measurements. Data represent mean ± SEM (n=23-25 mice  per group; **p&lt;0.01, Unpaired t-test)."
NCBI gene IDs:
"C2β: 240752"
Figure legend: "WT and C2βD1212A/D1212A mice were subjected to tMCAO followed by 24h of reperfusion. (B) Protein expression of TNFα, IL-1β and IL-6. Data represent mean ± SEM (n=4 mice per group; *p=0.05; Mann-Whitney test). Data represent mean ± SEM (n=5-7 mice per group; **p&lt;0.01; Mann-Whitney test)."
NCBI gene IDs:
"C2β: 240752"
Figure legend: "A Gene expression of TNFα (n=8-10 mice for WT and n=6 mice  for  C2βD1212A/D1212A), IL-1β (n=8 mice for WT and n=10-12  mice for C2βD1212A/D1212A), IL-6 (n=8 mice for WT and n=10-12 mice for C2βD1212A/D1212A) and VCAM-1  (n=8-10 mice for WT and n=10 mice for C2βD1212A/D1212A) after 1 hour of ischemia without reperfusion (right panel). Gene expression of IL-6 (n=10-12 mice for WT and n=8 mice for C2βD1212A/D1212A) and  VCAM-1 (n=12 mice for WT and n=10mice for C2βD1212A/D1212A) after 1 hour of ischemia followed by 4 hours of reperfusion (bottom, left panel) on WT and C2βD1212A/D1212A mice. The mRNA levels are given as the fold increase normalized to PGK1  relative to the healthy contralateral hemisphere of WT mice. Data represent mean ± SEM (***p&lt;0.001, **p&lt;0.01, *p&lt;0.05, Mann-Whitney test or unpaired t-test, as accordance)."
NCBI gene IDs:
"IL-1β: 16176
IL-6: 16193
PGK1: 18655
C2β: 240752
TNFα: 21926
VCAM-1: 22329"
Figure legend: "Representative photographs of coronal brain sections and graph quantification of  infarct volume (dashed white line) measurements in bone marrow  (BM) chimeric mice 24 hours after tMCAO. WT&gt;WT: transplantation  of WT BM into WT hosts; C2βD1212A&gt;WT: transplantation  of C2βD1212A/D1212A BM into WT hosts;  C2βD1212A&gt;C2βD1212A: transplantation of C2βD1212A/D1212A BM into C2βD1212A/D1212A hosts; WT&gt; C2βD1212A transplantation of WT BM into kinase-dead hosts. Data represent mean ± SEM; n=13-16 mice per group; *p&lt;0.05 **p&lt;0.01; ***p&lt;0.001 vs WT&gt;WT, Mann-Whitney test or unpaired t-test, as accordance."
NCBI gene IDs:
"C2β: 240752"
Figure legend: "B Analysis of APPL1, EEA1, LAMP1 and Rab11a staining in sh-Control and sh-PI3KC2β hCMEC/D3 cells. White dotted lines delineate cells; Scale bar, 10µm; nuclei are shown in blue (DAPI)."
NCBI gene IDs:
"PI3KC2β: 5287"
Figure legend: "(A) Representative VE-cadherin staining and graph quantification of fluorescence intensity normalized to total number of cells per field (DAPI  labelling) in sh-Control and sh-PI3KC2β hCMEC/D3 in resting condition and after 24 hours activation with 25 ng/ml of TNFα. Control normalized to 100%. Scale bar, 15 µm. Data represent mean ± SEM of cell fields randomly selected (dots n=14-19). Data are collected from 4-5 independent experiments; ***p&lt;0.001, unpaired t-test."
NCBI gene IDs:
"PI3KC2β: 5287"
Figure legend: "A Representative  images of PI3P and PI(3,4)P2 labeling and quantification using mCherry-FYVEHRS and GFP-PH(TAPP1) probes respectively in sh-Control or sh-PI3KC2β hCMEC/D3. Scale bar, 10 µm. Nuclei are shown in blue (DAPI). Staining intensity was quantified by ImageJ  software and normalized to total  number of cells (DAPI labelling) per field. Data represent mean ± SEM of cell fields randomly selected for PI3P (dots n=21) and for PI(3,4)P2 (dots n=14-16). Data were collected from at least 5 independent experiments; ***p&lt;0.001, unpaired t-test with Welch's correction."
NCBI gene IDs:
"PI3KC2β: 5287"
Figure legend: "C Graph quantification of APPL1-, EEA1- and Rab11- endosomes colocalized with mCitrin-VE-cadherin in sh-Control and sh-PI3KC2β hCMEC/D3 cells. Data are shown as percentage of total count  endosomes per cell. Data are expressed as mean ± SEM (n=33-35 cells for APPL1 and EEA1 endosomes and n=16-18 cells for Rab11 endosomes; 3 independent experiments); **p&lt;0.001, Mann-Whitney test or unpaired t-test, as accordance."
NCBI gene IDs:
"PI3KC2β: 5287"
Figure legend: "D Representative confocal microscopic images of VE-cadherin immunostaining in sh-Control cells, sh-PI3KC2β cells or sh-PI3KC2β cells transduced with Rab11S25N (Rab11-DN). Graph quantification of VE-cadherin; control normalized to 100%. Scale bar, 10 µm. Data represent mean ± SEM of cell fields randomly selected (dots n=6-9). Data are collected from at least 3 independent experiments; **p&lt;0.01 Mann-Whitney test."
NCBI gene IDs:
"PI3KC2β: 5287
Rab11: 8766
Rab11S25N: 8766"
Figure legend: "(C) Temporal analysis of antigen-specific CD8+ T cell responses. Peripheral blood was obtained on days 4, 7, 14, 21, 42 and 88 post-immunisation with WT (triangles; n=3-9), CSPSIINFEKL (orange squares; n=4-8) or UIS4SIINFEKL (blue circles; n=4-10) sporozoites, or no parasites (diamonds; n=2-5) and stained for Kb-SIINFEKL+ CD11ahi CD8+ T cells. Line graph shows mean values (±SEM) from representative experiments. (*, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; Welch's t-test comparing CSPSIINFEKL and UIS4SIINFEKL). See Appendix Table S1 for the number of mice used per timepoint and per group. See Appendix Table S4 for exact p-values."
NCBI gene IDs:
"CSP: 55148143
UIS4: 55148315"
Figure legend: "(D-H) C57BL/6 mice (n=4 per group), which received 2x106 CFSE-labelled OT-I splenocytes, were immunised with 10,000 γ-radiation attenuated WT, CSPSIINFEKL or UIS4SIINFEKL sporozoites intravenously. 5 days later, mice were sacrificed, spleens harvested and splenocytes assessed for (D) CFSE dilution of CD8+ T cells, (E) CFSE dilution of antigen experienced Kb-SIINFEKL+ CD11ahi CD8+ T cells and stained ex vivo (F-H) for effector CD8+ T cell surface markers. Shown are flow cytometry plots of Kb-SIINFEKL co-staining with markers of effector phenotypes: (F) CD11ahi, (G) CD62Llo and (H) CD49dhi."
NCBI gene IDs:
"CSP: 55148143
UIS4: 55148315"
Figure legend: "(B- C57BL/6 mice received 10,000 γ-radiation attenuated WT, CSPSIINFEKL or UIS4SIINFEKL sporozoites, either intravenously (n=6-8 mice per group) or (E- intradermally (n=4 mice per group). Additional control mice did not receive sporozoites (n=6-8 intravenously, n=4 intradermally). Spleens and livers were harvested either at day 14 (n=5 livers and n=6 spleens for intravenous immunisation) or day 42 (n=8 for intravenous immunisation), and IFN-γ-secreting lymphocytes following restimulation ex vivo with SIINFEKL peptide were quantified. Flow cytometry plots show representative percentages of CD8+ T cells co-stained with IFN-γ and CD11a (B, E)."
NCBI gene IDs:
"CSP: 55148143
UIS4: 55148315"
Figure legend: "(D) Protective efficacy as measured by quantitative real-time PCR. Groups of mice were vaccinated as described and challenged 19 days later with 10,000 WT (n=7 non-vaccinated, n=9 vaccinated), CSPSIINFEKL (n=6 non-vaccinated, n=4 vaccinated) or UIS4SIINFEKL sporozoites (n=3 non-vaccinated, n=11 vaccinated). 42 hours later livers were removed, and parasite load was assessed by qPCR. Plots show the relative parasite load of mice in each condition (**, p&lt;0.01; Mann-Whitney U test). See Appendix Table S4 for exact p-values. Data information: Data are representative of two experiments performed with scatter plots showing mean values (±SEM)."
NCBI gene IDs:
"CSP: 55148143
UIS4: 55148315"
Figure legend: "(A) Quantification of the amount of LC3‐II protein in mouse Rab‐knockdown cells. Representative blots are shown in supplementary Fig S1C online. Error bars represent the means and s.e.m. of three independent experiments."
NCBI gene IDs:
"Rab: 15463"
Figure legend: "(B) Quantification of the amount of p62 protein in candidates Rab‐knockdown cells. Representative blots are shown in supplementary Fig S1D online. Error bars represent the means and s.e.m. of three independent experiments."
NCBI gene IDs:
"Rab: 15463"
Figure legend: "(C,D) Control and Rab12‐knockdown MEFs were cultured under N or S conditions, fixed and then immunostained with the antibodies indicated. Representative images are shown in supplementary Fig S2 online. The mean numbers of LC3‐positive (C) or Atg16L1‐positive (D) dots per cell are shown. Error bars represent the means and s.e.m. of representative data (n≥80) from three independent experiments."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(E) MEFs that had been transfected with the control siRNA or Rab12 siRNA were cultured as in (C,D), and their lysates were analysed by immunoblotting with the antibodies indicated."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(G) Control and Rab12‐knockdown MEFs were cultured under N or S conditions in the absence or presence of 100 μM bafilomycin A1 for 1 h. Cell lysates were analysed by immunoblotting with the antibodies indicated, and the intensity of the LC3‐II band was quantified. The normalized amount (arbitrary units) of LC3‐II in lanes 1, 2, 3, 4, 5 and 6 is 1.0, 2.3, 4.3, 0.9, 1.6 and 3.8, respectively."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(H) MEFs transiently expressing mStr‐Rab12 were cultured under starved conditions and immunostained with the antibodies indicated. Scale bar, 20 μm. MEFs, mouse embryonic fibroblasts; N, nutrient‐rich; S, starved; siRNA, short interfering RNA. *P0.05; **P0.01; ***P0.005."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(A) Control and Rab12‐knockdown MEFs were cultured under nutrient‐rich or starved conditions, fixed and then immunostained with the antibodies indicated. Scale bar, 20 μm."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(B) Control and Rab12‐knockdown MEFs were cultured as in (A), and their lysates were analysed by immunoblotting with the antibodies indicated."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(C) Lysates of MEFs that had been transfected with control or Rab12 siRNA were analysed by immunoblotting with the antibodies indicated."
NCBI gene IDs:
"Rab12: 19328"
Figure legend: "(A) MEFs transiently co‐expressing Myc‐PAT4 and mStr‐Rab12 were immunostained with anti‐Myc antibody."
NCBI gene IDs:
"Rab12: 19328
PAT4: 234967"
Figure legend: "(B) MEFs transiently expressing Myc‐PAT4 were immunostained with the antibodies indicated. Scale bars, 20 μm."
NCBI gene IDs:
"PAT4: 234967"
Figure legend: "(C) Total cell lysates and surface biotinylated proteins from MEFs stably expressing HA‐PAT4 were analysed by immunoblotting with the antibodies indicated."
NCBI gene IDs:
"PAT4: 234967"
Figure legend: "(D) Lysates of MEFs stably expressing HA‐PAT4 that had been transfected with control or Rab12 siRNAs were analysed by immunoblotting with the antibodies indicated."
NCBI gene IDs:
"Rab12: 19328
PAT4: 234967"
Figure legend: "(F) PAT4 mRNA levels from MEFs transfected with control or Rab12 siRNA as revealed by reverse‐transcription PCR analyses. Gapdh was used as an internal control."
NCBI gene IDs:
"Rab12: 19328
PAT4: 234967"
Figure legend: "(G) Total cell lysates and surface biotinylated proteins from MEFs stably expressing HA‐PAT4 that had been transfected with control or Rab12 siRNA were analysed by immunoblotting with the antibodies indicated. Note that the amounts of plasma‐membrane‐localized PAT4 protein and TfR protein were higher in Rab12‐knockdown MEFs, whereas the total amounts of EGFR protein and plasma membrane‐localized EGFR protein in these cells were slightly lower, but the mechanisms responsible for these changes are unknown. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; MEFs, mouse embryonic fibroblasts; PAT4, proton‐coupled amino‐acid transporter 4; siRNA, short interfering RNA."
NCBI gene IDs:
"Rab12: 19328
PAT4: 234967"
Figure legend: "B. ERK71 mutants have elevated triacylglycerol (TAG) levels (N=4 replicates of ≥10 larvae/ replicate for each genotype). C. Fat body specific depletion of ERK7 by RNAi (BDSC 56939) leads to increased TAG levels (N=4 replicates of 10 larvae/replicate for each genotype). "
NCBI gene IDs:
"ERK7: 31877"
Figure legend: "D. Representative immunofluorescent images of lipid droplet (LipidTOX) and nuclear (DAPI) staining in control and ERK71 mutant fat bodies of third instar larvae. Scale bar: 50 µm. E. ERK71 mutant fat body cells contain more lipid droplets than control cells (N=30 cells for each genotype). "
NCBI gene IDs:
"ERK7: 31877"
Figure legend: "F. After fed [13C]glucose, the ERK71 mutant fat bodies display elevated [13C]TAG/PE and unlabeled TAG/PE molar ratios (measured by mass spectrometry-based lipidomics, N=3 replicates of 15 fat bodies/replicate, TAG=triacylglycerol, PE=phosphatidylethanolamine)"
NCBI gene IDs:
"ERK7: 31877"