EMBO/SourceData huggingface dataset:
Figure legend: "C Triple immunofluorescence  for GFP  (green), the cycling cell marker Ki67  (magenta) and TBR2  (yellow), combined with DAPI staining  (white), of a 59 days-old chimpanzee cerebral organoid  4 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+, Ki67+ and TBR2+ triple-positive cells. Scale bar, 50 µ D Quantification of the proportion of GFP+ cells in the SVZ/NL  that are Ki67+ and TBR2+ double-positive in 59 days-old chimpanzee cerebral organoids  4 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 8 control and 8 ARHGAP11B-transfected cerebral organoids  of four independent batches each; error bars indicate SD; *, P &lt;0.05 (one-sided Wilcoxon rank sum test)."
NCBI gene IDs:
"GFP: 
ARHGAP11B: 89839"
Figure legend: "A Double immunofluorescence  for GFP  (green) and the radial glia marker HOPX  (yellow), combined with DAPI staining  (white), of a 59 days-old chimpanzee cerebral organoid   4 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and HOPX+ double-positive cells. Scale bar, 50 µm. B Quantification of the proportion of GFP+ cells in the SVZ/NL  that are HOPX+ in 59 days-old chimpanzee cerebral organoids   4 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 10 control and 10 ARHGAP11B-transfected cerebral organoids  of three independent batches each; error bars indicate SD; **, P &lt;0.01 (one-sided Wilcoxon rank sum test). C Double immunofluorescence for GFP  (green) and HOPX  (yellow), combined with DAPI staining  (white), of a 61 days-old chimpanzee cerebral organoid   10 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and HOPX+ double-positive cells. Scale bar, 50 µm. D Quantification of the proportion of GFP+ cells in the SVZ/NL  that are HOPX+ in 61 days-old chimpanzee cerebral organoids  10 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 5 control and 7 ARHGAP11B-transfected cerebral organoids  of three independent batches each; error bars indicate SD; *, P&lt;0.05 (two-sided Student's t-test)."
NCBI gene IDs:
"GFP: 
ARHGAP11B: 89839"
Figure legend: "A Double immunofluorescence  for GFP (green) and the neuron  marker Hu  (magenta), combined with DAPI staining  (white), of a 61 days-old chimpanzee cerebral organoid  10 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and Hu+ double-positive cells. Scale bar, 50 µm. B Quantification of the proportion of GFP+ cells that are Hu+ in 61 days-old chimpanzee cerebral organoids  10 days after electroporation  with GFP expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 7 control and 11 ARHGAP11B-transfected cerebral organoids of two independent batches each; error bars indicate SD; **, P &lt;0.01 (two-sided Student's t-test)."
NCBI gene IDs:
"GFP: 
ARHGAP11B: 89839"
Figure legend: "C Double immunofluorescence  for GFP  (green) and the  deep-layer neuron  marker CTIP2  (magenta), combined with DAPI staining  (white), of a 59 days-old chimpanzee cerebral organoid  4 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and CTIP2+ double-positive cells. Scale bar, 50 µm. D Quantification of the proportion of GFP+ cells in the SVZ/NL  that are CTIP2+ in 59-days old chimpanzee cerebral organoids  4 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 10 control and 10 ARHGAP11B-transfected cerebral organoids   of two independent batches each; error bars indicate SD; ***, P &lt;0.001 (two-sided Student's t-test)."
NCBI gene IDs:
"GFP: 
ARHGAP11B: 89839"
Figure legend: "E Double immunofluorescence  for GFP  (green) and the upper-layer neuron  marker SATB2  (magenta), combined with DAPI staining  (white), of a 66 days-old chimpanzee cerebral organoid   15 days after electroporation  with GFP  expression plasmid plus either control plasmid (top) or ARHGAP11B  expression plasmid (bottom). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and SATB2+ double-positive cells. Scale bar, 50 µm. F Quantification of the proportion of GFP+ cells in the SVZ/NL  that are SATB2+ in 66 days-old chimpanzee cerebral organoids  15 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark red) or ARHGAP11B  expression plasmid (light red). Data are the mean of 6 control and 4 ARHGAP11B-transfected cerebral organoids   of two independent batches each; error bars indicate SD; **, P &lt;0.01 (one-sided Wilcoxon rank sum test)."
NCBI gene IDs:
"GFP: 
ARHGAP11B: 89839"
Figure legend: "A Double immunofluorescence  for GFP  (green) and the thymidine  analog BrdU  (magenta, combined with DAPI staining  (white), of 57 days-old human (top panels) and chimpanzee  (bottom panels) cerebral organoids   2 days after electroporation  with GFP  expression plasmid plus either control plasmid (upper panels) or ARHGAP11A220  expression plasmid (lower panels). Tick marks indicate the borders of the VZ   and SVZ/NL; arrowheads indicate examples of GFP+ and BrdU+ double-positive cells. Scale bar, 50 µm. B Quantification of the proportion of GFP+ cells in the SVZ/NL  that are BrdU+ in 57 days-old human  (blue) and chimpanzee  (red) cerebral organoids   2 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark blue and dark red) or ARHGAP11A220 expression plasmid (light blue and light red). Data are the mean of 12 human  control, 12 human ARHGAP11A220-transfected, 9 chimpanzee  control and 8 chimpanzee ARHGAP11A220-transfected  cerebral organoids  (grown from one human  and one chimpanzee iPSC  line, respectively) of six (human) and four (chimpanzee) independent batches each; error bars indicate SD; n.s., not significant; **, P &lt;0.01 (Kruskal Wallis test)."
NCBI gene IDs:
"GFP: 
ARHGAP11A: 9824"
Figure legend: "C Double immunofluorescence  for GFP  (green) and TBR2  (yellow), combined with DAPI staining  (white), of 57 days-old human  (top panels) and chimpanzee  (bottom panels) cerebral organoids   2 days after electroporation  with GFP   expression plasmid plus either control plasmid (upper panels) or ARHGAP11A220 expression plasmid (lower panels). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and TBR2+ double-positive cells. Scale bar, 50 µm. D Quantification of the proportion of GFP+ cells in the SVZ/NL  that are TBR2+ in 57 days-old human  (blue) and chimpanzee  (red)  cerebral organoids   2 days after electroporation  with GFP  expression plasmid plus either control plasmid (dark blue and dark red) or ARHGAP11A220 expression plasmid (light blue and light red). Data are the mean of 9 human  control, 9 human ARHGAP11A220-transfected, 10 chimpanzee  control and 10 chimpanzee ARHGAP11A220-transfected cerebral organoids   (grown from one human and one chimpanzee iPSC  line, respectively) of four (human) and three (chimpanzee) independent batches each; error bars indicate SD; n.s., not significant; *, P &lt;0.05 (Kruskal Wallis test)."
NCBI gene IDs:
"GFP: 
ARHGAP11A: 9824"
Figure legend: "A Double immunofluorescence  for GFP  (green) and the bRG  marker PTPRZ1  (magenta), combined with DAPI staining  (white), of 60 days-old human forebrain organoids, generated from ARHGAP11A  plus ARHGAP11B  double-knockout clone 16. Images were obtained 10 days after electroporation  with GFP  expression plasmid plus either ARHGAP11A  plus ARHGAP11B  expression plasmids (ARHGAP11A+B-rescue, referred to as Control), ARHGAP11B  expression plasmid only (ARHGAP11B-rescue, referred to as ARHGAP11A-KO), or ARHGAP11A  expression plasmid only (ARHGAP11A-rescue, referred to as ARHGAP11B-KO). Tick marks indicate the borders of the VZ  and SVZ/NL; arrowheads indicate examples of GFP+ and PTPRZ1+ double-positive cells. Scale bar, 50 µm B Quantification of the proportion of GFP+ cells in the SVZ/NL  that are PTPRZ1+ in 60 days-old ARHGAP11A  plus ARHGAP11B  double-knockout (11A+11B KO) human forebrain organoids. Organoids  were generated from clone 16 (left) and clone j (right), and data were obtained 10 days after the indicated electroporation  with GFP  expression plasmid plus either ARHGAP11A  (11A) plus ARHGAP11B  (11B) expression plasmids (referred to as Control, as this constitutes an ARHGAP11A+B double-rescue in the 11A+11B KO organoids; dark blue), ARHGAP11B  (11B) expression plasmid only (referred to as 11A-KO, as this constitutes a selective ARHGAP11B  rescue in the 11A+11B KO organoids; light blue), or ARHGAP11A  (11A) expression plasmid only (referred to as 11B-KO, as this constitues a selective ARHGAP11A  rescue in the 11A+11B KO organoids; dark red). For clone 16, data are the mean of 8 control, 8 11A-KO and 8 11B-KO human forebrain organoids  of two independent batches each; for clone j, data are the mean of 9 control, 10 11A-KO and 8 11B-KO human forebrain organoids   of three independent batches each; error bars indicate SD; n.s., not significant; *, P &lt;0.05; **, P &lt;0.01; ***, P &lt;0.001 (Kruskal Wallis test)"
NCBI gene IDs:
"GFP: 
ARHGAP11A: 9824
ARHGAP11B: 89839"
Figure legend: "Images and comparison of VEGFR3 expression (presented as relative fluorescent intensity (FI)) in CD31+/LYVE-1+ lacteals of jejunum between WT and VR3iΔLEC mice. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). AU, arbitrary unit. Scale bars, 100 μm."
NCBI gene IDs:
"VR3: 14257"
Figure legend: "Images and comparisons of absolute and relative lacteal lengths in duodenum (DD), jejunum (JJ) and ileum (IL) of WT and VR3iΔLEC mice. Note that, in addition to the alterations in the lacteal lengths in VR3iΔLEC mice, diameters of the lacteal also shrank by VEGFR3 ablation. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). Scale bars, 100 μm."
NCBI gene IDs:
"VEGFR3: 14257
VR3: 14257"
Figure legend: "Images and comparisons of number of Prox1+ LECs within initial 100 μm portion in CD31+/LYVE-1+ lacteals of jejunum from WT and VR3iΔLEC mice. Dotted lines outline villi. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). Scale bars, 100 μm."
NCBI gene IDs:
"VR3: 14257"
Figure legend: "Images and comparison of proportions of each VE-cadherin LEC junctions in CD31+/LYVE-1+ lacteals in jejunum from WT and VR3iΔLEC mice. Button-like (red arrowheads) and zipper-like (open arrowheads) junctions are indicated in each magnified box in below (n = 6 mice/group, 5-10 villi/mouse). Scale bars, 25 μm."
NCBI gene IDs:
"VR3: 14257"
Figure legend: "Comparisons of serum triglyceride (TG) at indicated time points in WT and VR3iΔLEC mice (n = 6 mice/group)."
NCBI gene IDs:
"VR3: 14257"
Figure legend: "Comparison of mRNA levels of VEGF-C in the whole tissue of jejunum and ileum from SPF, GF, and CONV mice (n = 3-5 mice/group)."
NCBI gene IDs:
"VEGF-C: 22341"
Figure legend: "Comparison of mRNA levels of VEGF-C in sorted CD45- stromal cells and CD45+ MHCII+ F4/80+ macrophages from jejunum and ileum of vehicle-treated mice. The mean of transcription levels of VEGF-C in CD45- stromal cells was normalized to 1, and the relative levels in macrophages were presented as fold change (n = 4 mice/group)."
NCBI gene IDs:
"VEGF-C: 22341"
Figure legend: "Comparisons of VEGF-C transcription level in CD45- stromal cells (left) and CD45+ MHCII+ F4/80+ macrophages (right) in jejunum and ileum of vehicle- and ABX-treated mice. The mean of transcription levels of VEGF-C in vehicle-treated mice was normalized to 1, and the relative levels in ABX-treated mice were presented as fold change (n = 5 -6 mice/group)."
NCBI gene IDs:
"VEGF-C: 22341"
Figure legend: "Representative flow cytometric analysis and comparison of MHCII+ F4/80+ macrophage gated on DAPI- CD45+ cells from jejunum and ileum of WT and DTR mice (n = 5 mice/group)."
NCBI gene IDs:
"DTR: 735979"
Figure legend: "Comparison of mRNA levels of VEGF-C in the whole tissue of jejunum and ileum from WT and DTR mice (n = 5 mice/group)."
NCBI gene IDs:
"DTR: 735979
VEGF-C: 22341"
Figure legend: "Images and comparisons of absolute and relative lacteal lengths in duodenum (DD), jejunum (JJ) and ileum (IL) of WT and DTR mice. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). Scale bars, 100 μm."
NCBI gene IDs:
"DTR: 735979"
Figure legend: "Images and comparisons of number of Prox1+ LECs within initial 100 μm portion (upper) and VEGFR3 expression (lower, presented as relative fluorescent intensity (FI)) in CD31+/LYVE-1+ lacteals of jejunum from WT and DTR mice. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). AU, arbitrary unit. Scale bars, 100 μm."
NCBI gene IDs:
"DTR: 735979"
Figure legend: "Images and comparison of proportions of each VE-cadherin LEC junctions in CD31+/LYVE-1+ lacteals in jejunum between WT and DTR mice. Button-like (red arrowheads) and zipper-like (open arrowheads) junctions are indicated in each magnified box in below (n = 6 mice/group, 5-10 villi/mouse). Scale bars, 25 μm."
NCBI gene IDs:
"DTR: 735979"
Figure legend: "Representative flow cytometric analysis and comparison of MHCII+ F4/80+ macrophage gated on DAPI- CD45+ cells from jejunum and ileum of WT and MyD88ΔMP mice (n = 5 mice/group)."
NCBI gene IDs:
"MyD88: 17874"
Figure legend: "Comparisons of mRNA levels of VEGF-C in sorted MHCII+ F4/80+ macrophages from jejunum and ileum of WT and MyD88ΔMP mice. The mean of transcription levels of VEGF-C in WT mice was normalized to 1, and the relative level in MyD88ΔMP mice were presented as fold change (n = 5 mice/group)."
NCBI gene IDs:
"MyD88: 17874
VEGF-C: 22341"
Figure legend: "Images and comparisons of absolute and relative lacteal lengths in duodenum (DD), jejunum (JJ) and ileum (IL) of WT and MyD88ΔMP mice. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). Scale bars, 100 μm."
NCBI gene IDs:
"MyD88: 17874"
Figure legend: "Images and comparisons of number of Prox1+ LECs within initial 100 μm portion (upper) and VEGFR3 expression (lower, presented as relative fluorescent intensity (FI)) in CD31+/LYVE-1+ lacteals of jejunum from WT and MyD88ΔMP mice. Each dot indicates mean value of 5-10 villi. Each dot indicates mean value of 5-10 villi in a mouse (n = 6 mice/group). AU, arbitrary unit. Scale bars, 100 μm."
NCBI gene IDs:
"MyD88: 17874"
Figure legend: "Images and comparison of proportions of each VE-cadherin LEC junctions in CD31+/LYVE-1+ lacteals in jejunum between WT and MyD88ΔMP mice. Button-like (red arrowheads) and zipper-like (open arrowheads) junctions are indicated in each magnified box in below (n = 6 mice/group, 5-10 villi/mouse). Scale bars, 25 μm."
NCBI gene IDs:
"MyD88: 17874"
Figure legend: "A-C: JurkatT cells were transfected with control, FIP3.1 or FIP3.2 siRNA oligonucleotides. A: Intracellular distribution of endogenous Rab11 and Rac1 was assessed by immunofluorescence."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "Jurkat T cells were transfected with control, FIP3.1 or FIP3.2 siRNA oligonucleotides. A: Intracellular distribution of endogenous Rab11 and Rac1 was assessed by immunofluorescence.A, E, G: Three-dimensional (3D) confocal images were post-treated by deconvolution. A 0.4-µ-thick medial stack is shown. The pericentrosomal vesicular compartment is zoomed at the bottom right-hand corner.B, F, H: Population analysis of the co-localization volume between Rab11 and Rac1 within the percentriolar vesicular compartment. Each dot represents one cell. Horizontal bars represent the mean ± SEM, Mann-Whitney test was used. Images are representative of three experiments. Bar, 5 µm"
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "E, F: Jurkat cells were transfected with expression vectors encoding GFP, GFP-FIP3-WT or GFP-FIP3-I738E. Endogenous Rac1 was detected by immunofluorescence."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "G: Jurkat cells were transfected with expression vectors encoding GFP, Rab11WT-GFP, or Rab11Q70L-GFP (constitutively active) or Rab11S25N-GFP (dominant negative) mutants. Endogenous Rac1 was detected by immunofluorescence.A, E, G: Three-dimensional (3D) confocal images were post-treated by deconvolution. A 0.4-µ-thick medial stack is shown. The pericentrosomal vesicular compartment is zoomed at the bottom right-hand corner.B, F, H: Population analysis of the co-localization volume between Rab11 and Rac1 within the percentriolar vesicular compartment. Each dot represents one cell. Horizontal bars represent the mean ± SEM, Mann-Whitney test was used. Images are representative of three experiments. Bar, 5 µm"
NCBI gene IDs:
"Rab11: 9230///8766"
Figure legend: "A, B: Effect of FIP3 silencing on Rac1 expression. Jurkat cells were transfected with control, FIP3.1 or FIP3.2 siRNA. Three days later cells were lysed and the expression of FIP3, Rac1 and β-tubulin analyzed by western blot. The intensity of bands corresponding to FIP3 and to Rac1 was quantified by ImageJ and normalized to the β-tubulin band used as loading control. The mean ± SD of the percentage of band intensity in silenced cells with respect to controls of three independent experiments is shown."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "B: Rac1 interaction with FIP3: HEK293T cells were transfected with GFP, GFP-FIP3 WT or GFP-FIP3I738E expression vectors. 24 h later, cells were lysed and immunoprecipitated with anti-GFP Ab. Immunoprecipitates were analyzed by electrophoresis and western blot using anti-GFP Ab (top panel) or anti-Rac1 Ab (bottom panel). Total cell lysates were analyzed (100,000 cells). The position of Rac1 (20 kDa) and Ig light chain (25 kDa) polypeptides is depicted on the right side. Arrowheads at the bottom point the bands corresponding to Rac. Quantification of bands was performed with ImageJ as the percentage of intensity with respect to the total lysate bands on the left, shown below. A representative experiment out of three carried out is shown."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "JurkatT cells were transfected with expression vectors encoding: A, C: GFP, GFP-FIP3WT, or GFP-FIP3I738E. B, C: Rab11WT-GFP, Rab11Q70L-GFP (constitutively active), or Rab11S25N-GFP (dominant negative)."
NCBI gene IDs:
"FIP3: 8517
Rab11: 9230///8766"
Figure legend: "C: Rab11WT-GFP, Rab11Q70L-GFP (constitutively active), or Rab11S25N-GFP (dominant negative)."
NCBI gene IDs:
"Rab11: 9230///8766"
Figure legend: "D, E: or transfected with control, or FIP3.1 siRNA. Cells were allowed to form immunological synapses with SEE-pulsed Raji cells for 30 min. Intracellular distribution of endogenous Rac1 and surface distribution of CD3 were detected by immunofluorescence. 3D confocalimages were post-treated by deconvolution. A 0.4-µm-thick medial stack is shown. C, E: Population analyses of the amount of Rac1 at the immunological synapse relative to the total cellular Rac1. Each dot corresponds to one cell. Horizontal bars represent the mean ± SEM. Mann-Whitney test. Images are representative of three experiments. Bar, 5 µm."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "JurkatT cells were transfected with either control or FIP3.1 siRNA. A: Cells were allowed to form immunological synapses with SEE-pulsed Raji cells for 30 min and endogenous Lck was detected by immunofluorescence. 3D confocalimages were post-treated by deconvolution. A 0.4-µm-thick medial stack is shown."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "A, B, C: JurkatT cells transfected with siRNA control, FIP3.1 or FIP3.2 were allowed to spread on poly-lysine-coated coverslips for the indicated times. Intracellular actin was detected with FITC-coupled Phalloidin. A: A single optical section at the level of the contact surface is shown. Bar, 5 µm. B: Population analyses of the spreading area of cells at the level of the contact surface. Each dot represents one cell. Horizontal bars represent the mean ± SEM. C:Time course representation of B, showing mean ± SEM."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "D-F: Primary humanCD4T cells were transfected with control or FIP3.1 siRNA. D, E: Cells were allowed to spread on poly-lysine-coated coverslips for 5 min. Intracellular Lck was detected by immunofluoresecence. D: A single optical section at the level of the contact surface is shown. Bar, 10 µm. E: Population analyses of the spreading area of cells at the level of the contact surface."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "Jurkat T cells were transfected with control or FIP3.1 siRNA, then transfected again with empty vector or with expression vectors encoding Myc-tagged-Rac1WT, -Rac1G12V (constitutively active mutant), or -Rac1Rac1T17N (dominant negative mutant). Cells were allowed to spread on poly-lysine-coated coverslips for the indicated time and the spreading area of cells measured. A-C, H: Population analyses of the spreading area of cells at the level of the contact surface. A-C: Time course plots representing the mean ± SEM of the data shown as in I. D-G: Staining for nucleus (blue = Dapi), F-actin (green = phalloidin) and Rac1-Myc (red = anti-Myc) is shown. A single optical section at the level of the contact surface is shown. Bar, 5 µm."
NCBI gene IDs:
"FIP3: 8517
Rac1: 5879"
Figure legend: "A: Jurkat T cells transfected with control or FIP3.1 were treated 1 h with the Rac1 inhibitor NSC23766 at 100 µM and allowed to spread on poly-lysine-coated coverslips for 15 min. F-actin was detected with FITC-Phalloidin and the spreading area of cells at the contact surface was measured."
NCBI gene IDs:
"FIP3: 8517
Rac1: 5879"
Figure legend: "A: Jurkat T cells transfected with control or FIP3.1 were activated on anti-CD3-coated plastic plates in the presence of soluble anti-CD28 during 16 h. Concentration of IL2 released in the supernatant was measured by ELISA. Histograms represent the mean ± SD of three experiments. Mann-Whitney test."
NCBI gene IDs:
"FIP3: 8517"
Figure legend: "Cyclin E-expressing BJ cells were grown for 7 days with and without folate.Fork rate (Kb/min) distribution. White bars: BJ cells expressing an empty vector (n = 145); dark gray bars: BJ cells expressing the cyclin E oncogene (n = 147); light gray bars: BJ cells cultured for 7 days in a folate-free medium (n = 135); black bars: BJ cells expressing the cyclin E oncogene cultured for 7 days in a folate-free medium (n = 138)."
NCBI gene IDs:
"Cyclin E: 9134///898
cyclin E: 9134///898"
Figure legend: "Cyclin E-expressing BJ cells were grown for 7 days with and without folate. Fork distance distribution (Kb). The color code is as in (A). Empty vector (n = 78); CycE (n = 79); empty vector −folate (n = 71); CycE −folate (n = 80)."
NCBI gene IDs:
"CycE: 9134///898
Cyclin E: 9134///898"
Figure legend: "Cyclin E-expressing BJ cells were grown for 7 days with and without folate. Percent of origins with the indicated progression ratio between sister forks. Empty vector (n = 158); CycE (n = 155); empty vector −folate (n = 160); CycE −folate (n = 154). *P &lt; 0.05."
NCBI gene IDs:
"CycE: 9134///898
Cyclin E: 9134///898"