EMBO/SourceData huggingface dataset:
Figure legend: "(F) Fluorescence microscopy  detection of DHE-derived fluorescence (red) in the SON. (G) Quantification of DHE-derived fluorescence in the SON  [n=8 SON  per group; each group consisted of 4 mice]. Data information: AVP: Vasopressin, d: day, DHE: dihydroethidium  , opt: optic tract, SON: Supraoptic nucleus,. Scale bar=20μm. For panels G data are represented as mean ± SEM. G: unpaired t-test; ; *p&lt;0.05, ***p&gt;0.001."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998"
Figure legend: "(J) Calcium  recordings in AVP magnocellular terminals 10 minutes before and one hour after the i.p injection of insulin  (red and orange) or saline  (black). The vertical arrow corresponds to the i.p. injection (time=0). (K) Quantification of mean signal intensity in 10-minute time  bins. [n=6-8 mice  per group]. Data information: AUC: area under the curve, AVP: Vasopressin INS: insulin, SAL: saline  For panels K, data are represented as mean ± SEM. K: 1-way ANOVA with Tukey's post-hoc test; *p&lt;0.05, ***p&gt;0.001."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998"
Figure legend: "(B) Immunofluorescence microscopy  detection of AVP  in the PVN  and the SON. Data information: 3V: 3rd ventricle, AVP: Vasopressin, KO: AVPTM117KO opt: optic tract, PVN: paraventricular nucleus, SON: Supraoptic nucleus, WT: AVPTM117WT. Scale bar=100μm."
NCBI gene IDs:
"AVP: 11998
TM117: 320709"
Figure legend: "(C) Quantitation of AVP  positive cells in the PVN  [n=3-4 mice  per group]. (D) Quantitation of AVP  positive cells in the SON [n=3-4 mice  per group]. Data information: AVP: Vasopressin, KO: AVPTM117KO WT: AVPTM117WT. For panels C and D lines correspond to the mean value per group and error bars represent ± SEM. For panels C,D: unpaired t-test; ; *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"AVP: 11998
TM117: 320709"
Figure legend: "(E) Glycemia  one hour after saline  (black) or insulin  (red) i.p. injections in AVPTM117WT and AVPTM117KO male mice  [n=8-9 mice  per group]. (F) CPP plasma  levels one hour after saline  or insulin  injection in AVPTM117WT and AVPTM117KO male mice  [n=8 mice  per group]. (G) GCG plasma  levels one hour after saline  or insulin  injection in AVPTM117WT and AVPTM117KO male mice  [n=8-9 mice  per group]. Data information: AVP: Vasopressin, d: day, INS: insulin, KO: AVPTM117KO, SAL: saline, WT: AVPTM117WT. For panels E-G bars correspond to the mean value per group and error bars represent ± SEM. E-G: 2-way ANOVA for each timepoint in comparison to SAL  baseline with Bonferroni post-hoc test; *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998
TM117: 320709"
Figure legend: "Green line: Normalized population expression level averaged over all OxyR, and SoxS-regulated promoters; red line: average over all LexA-regulated promoters as measured with a promoter-GFP plasmid library in a plate reader. Oxidative stress promoters clearly precede SOS response promoters in response to NIT stress, when measured at the population level Error bars show standard deviation over all detected oxidative stress and SOS promoters, respectively It is not clear if this temporal order correctly reflects the temporal order in single cells. B. Schematic showing response of two different genes (green and red) in three different cells (solid, dashed, dotted line). The temporal order observed at the population level correctly reflects the temporal order in each individual cell. C. As B. Here the temporal order at the population level is not the same in every single cell: Although in most cells, the green gene responds before the red one, one cell expresses the green gene before the red one (dashed lines)."
NCBI gene IDs:
"GFP: "
Figure legend: "Single-cell gene expression time traces from the dnaK promoter in response to TET stress, normalized to the median full response. Response times were determined as the time point at which a threshold expression level was reached This threshold was low enough so that most cells exceed it and high enough to avoid false positives due to the low signal-to-noise ratio at time point zero. Brown line: median of all cells. Time traces are from one microcolony. B. Histogram of the response times for the dnaK promoter with mean µt = 1.87 h and standard deviation σt = 0.37 h, and fit of an Erlang distribution with shape parameter n = 37 Response times are from two microcolonies."
NCBI gene IDs:
"dnaK: 944750"
Figure legend: "C. Standard deviation σt versus mean response time µt for 23 different promoters in three antibiotic stress conditions (TMP, TET, and NIT). The standard deviation of the response time σt grows with the mean response time µt and does not fall below a 'precision limit' (dashed line) that increases linearly with a slope ~0.165. The dotted line indicates the upper bound to timing variability where σt = µt, see text. The promoter dnaK under TET has low timing variability, whereas the promoters recA, fpr, osmC, and wrbA under TMP stress have high timing variability. The response time mean and standard deviation are from subsampling of descendants of single cells that were present at the time of stress addition Subsampling for each promoter was done from at least 2 microcolonies and the descendants of at least 17 individual cells present at the time of stress addition."
NCBI gene IDs:
"dnaK: 944750
fpr: 948414
osmC: 946043
recA: 947170
wrbA: 947263"
Figure legend: "E. Standard deviation σt versus mean response time µt for the LlacO-1 promoter induced with different IPTG concentrations."
NCBI gene IDs:
"LlacO-1: "
Figure legend: "D. Response times of the LlacO-1 promoter (Lutz &amp; Bujard, 1997), induced with 1 mM IPTG at time zero, measured with YFP and CFP constructs in the same cell (r = 0.81 ± 0.04, p = 6 · 10-12)."
NCBI gene IDs:
"LlacO-1: 
CFP: 
YFP: "
Figure legend: "A. Normalized expression from the promoters ybjC and recA in response to NIT stress, measured at the population level using GFP reporters 4 μg/mL NIT was added at time zero. The oxidative stress promoter ybjC clearly precedes the SOS response promoter recA, when measured at the population level Error bars show standard deviation of three replicate experiments measured on different days. B. Normalized ybjC and recA expression over time in response to the addition of 4 μg/mL NIT at time zero in single cells from one microcolony. Dashed line: threshold used to determine response times Lower panels, upper row: Dual-color images of the oxidative stress reporter ybjC (green) and the SOS reporter recA (red) in single cells at 4 different time points after NIT addition in one microcolony. Note that the ybjC signal (green) is stronger at t = 2h, whereas the recA signal (red) is only apparent at t = 4h and 6h. Lower row: Constitutively expressed mCherry used for segmentation. C. Response times for ybjC and recA from individual cells with dual reporters (Figure 3), combined from two microcolonies. All response times for recA are higher than for ybjC and response times for both reporters are strongly correlated (r = 0.74 ± 0.04, p = 3.8 · 10-6). The error of the correlation coefficient is from subsampling of descendants of single cells that were present at the time of NIT addition"
NCBI gene IDs:
"GFP: 
mCherry: 
recA: 947170
ybjC: 945481"
Figure legend: "A. Expression from the gadB and recA promoters, measured at the population level using GFP reporters over time; 0.5 μg/mL TMP was added at time zero. The acid stress response (gadB) clearly precedes the SOS response (recA). Error bars show standard deviation of three replicate experiments measured on different days. B. Normalized expression of gadB and recA expression over time in individual cells responding to the addition of 0.5 μg/mL TMP at time zero shown for one microcolony. Dashed line: threshold used to determine response times (Methods). Lower panels, upper row: Dual-color images of gadB (blue) and recA (red) in single cells at 4 different time points. Most cells are predominantly either red or blue, i.e. acid stress and SOS response are typically not both strongly expressed in the same cells. Lower row: Constitutively expressed mCherry used for segmentation. C. Scatter plot of response times for gadB and recA in single cells; there is no clear temporal order and no significant correlation (r = 0.08 ± 0.08, p = 0.68); data is combined from three microcolonies. Error is from subsampling of descendants of single cells present at the time point of TMP addition"
NCBI gene IDs:
"GFP: 
mCherry: 
gadB: 946058
recA: 947170"
Figure legend: "D. Expression of gadB and recA in inosine-supplemented medium, averaged over 13 single cells in a microcolony. Insets: Dual color image of gadB and recA and segmentation image at t = 6h."
NCBI gene IDs:
"gadB: 946058
recA: 947170"
Figure legend: "(A) Srcap expression in murine different tissues was examined by Northern blot. A 277 nt probe of Srcap (81-358nt) was labeled for Northern blot analysis. 18S RNA was used as a loading control."
NCBI gene IDs:
"18S RNA: 
Srcap: 100043597"
Figure legend: "(B) Two-week-old SRCAP-C-HA tag mice were sacrificed for longitudinal sections followed by immunofluorescence staining. A global look of the section was shown. Scale bar: 500 μm. Green: EpCAM, Red: HA tag, Nuclei were counterstained by DAPI."
NCBI gene IDs:
"HA: 
SRCAP: 100043597"
Figure legend: "(D) Duodenum region of intestinal tissues were obtained from Lgr5GFP-CreERT2 (Lgr5GFP) mice for immunofluorescence staining by indicated antibodies with OpalTM 7-color fIHC kit. Nuclei were counterstained with DAPI. Scale bar: 50 μm."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Lgr5: 14160"
Figure legend: "(E) ISCs from Lgr5GFP mice were collected for organoid formation. Organoids were fixed for immunofluorescence staining with indicated antibodies. Scale bar: 50 μm. Data information: Green: EpCAM, Red: SRCAP, Purple: PCNA, Nuclei were counterstained by DAPI."
NCBI gene IDs:
"GFP: 
Lgr5: 14160"
Figure legend: "(A) Representative images of intestines from 8-week-old Srcap+/+ and Srcap-/- mice (n=6 per group). Length of 120 villi and 120 crypts (20 villi and 20 crypts per mouse) was measured and shown in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. Scale bar, 100 μm. Dotted lines indicate the boundary between crypts and villi. Arrows indicate the length of crypts or villi."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(B) Intestinal tissues from 8-week-old Srcap+/+ and Srcap-/- mice (n=6 per group) were collected for immunofluorescence staining with indicated antibodies. Typical images were shown in left panel and statistical ISC numbers from 120 crypts (20 crypts per mouse) were shown in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. Scale bar, 50 μm."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(D) Srcap+/+ and Srcap-/- mice were treated with 10 Gy's radiation and sacrificed at different time points. Intestinal tissues were collected for H&amp;E staining. Numbers of intact crypts were shown in lower panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. 50 fields (10 fields per mouse) were taken for each group (n=5). Scale bars, 100 μm."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(E) Lgr5GFP-CreERT2;Rosa26lsl-YFP (LRYFP) were crossed with Srcapflox/flox mice, followed by administration of tamoxifen (TAM) for lineage tracing analysis. Mice were sacrificed 7 days after TAM induction and typical jejunum sections were stained. Numbers of traced crypt-villus units were shown in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. 50 fields (10 fields per mouse) were taken for each group (n=5). Scale bars, 50 μm."
NCBI gene IDs:
"YFP: 
GFP: 
Cre: 2777477
Rosa26: 14910
Lgr5: 14160
Srcap: 100043597"
Figure legend: "(F) Proliferation of Srcap+/+ and Srcap-/- ISCs were examined by Ki67 staining followed by flow cytometry analysis. Percentages of proliferated cells were counted as means ± S.D (lower panel). ** P &lt; 0.01 by two-tailed Student's t-test. n=5 per group."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(G) Multiple color staining was performed in Srcap+/+ and Srcap-/- intestine sections. AP-Red, DAB and AP-Blue were used for immunohistochemistray coloration. Numbers of each kind of epithelial cells from 50 crypts or 50 villi (10 crypts and 10 villi per mouse) were shown in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. Scale bars, 50 μm."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(D) Flag-tagged REST fragments were expressed in 293T and purified by immunoprecipitation with anti-Flag antibody. REST proteins were incubated with crypt lysates for pulldown followed by immunoblotting."
NCBI gene IDs:
"Flag: 
REST: 19712"
Figure legend: "(B) Transcriptome microarray analysis between Srcap-/- ISCs and WT ISCs. Top ten downregulated TFs in Srcap-/- ISCs were listed."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(C) Prdm16 expression was tested in Srcap+/+ and Srcap-/- ISCs by Western blotting."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(E) Prdm16 promoter (-875~-854) region was used as probe to perform DNA-pulldown assay with murine intestinal crypt lysates followed by immunoblotting."
NCBI gene IDs:
"Prdm16: 70673"
Figure legend: "(F, G) Indicated promoter activation was analyzed with luciferase assay. DKK1 was used as a control. Results of relative fold changes are shown as means ± S.D. Data represent five independent replicates. . ** p&lt;0.01 by two-tailed Student's t-test. ."
NCBI gene IDs:
"DKK1: "
Figure legend: "(A) WT and Srcap-/- ISCs were conducted for transcriptome microarray, followed by Gene Set Enrichment Analysis (GSEA) analysis for PPARδ target genes."
NCBI gene IDs:
"Srcap: 100043597"
Figure legend: "(B) Expression levels of PPARs and PPAR target genes were assessed in sorted ISCs by quantitative RT-PCR. Primers were listed in Table EV1. Relative gene expression folds were normalized to endogenous β-actin and shown as means ± S.D. Data represent five independent replicates. ** p&lt;0.01 and *** p&lt;0.001 by two-tailed Student's t-test."
NCBI gene IDs:
"β-actin: 11461"
Figure legend: "(D) ISCs were collected for organoid formation followed by quantitative RT-PCR. For Prdm16 overexpression, ISCs were infected with lentivirus carrying Prdm16 overexpressing plasmids. Primers were listed in Table EV1. Relative gene expression folds were normalized to endogenous β-actin and shown as means ± S.D. Data represent five independent replicates. ** p&lt;0.01 by two-tailed Student's t-test."
NCBI gene IDs:
"β-actin: 11461
Prdm16: 70673"
Figure legend: "(E) mRNA levels of ISC signature genes were examined by quantitative RT-PCR. Primers were listed in Table EV1. Relative gene expression folds were normalized to endogenous β-actin and shown as means ± S.D. Data represent five independent replicates."
NCBI gene IDs:
"β-actin: 11461"
Figure legend: "(A) Representative images of intestines from 8-week-old Rest+/+ and Rest-/- mice (n=6 per group). Length of 120 villi and 120 crypts (20 villi and 20 crypts per mouse) were measured in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. Scale bar, 100 μm. Dotted lines indicate the boundary between crypts and villi. Arrows indicate the length of crypts or villi."
NCBI gene IDs:
"Rest: 19712"
Figure legend: "(C) Intestine tissues from 8-week-old Rest+/+ and Rest-/- mice (n=6 per group) were collected for immunohistochemistry staining. Typical images were shown in left panel and statistical ISC numbers from 120 crypts (20 crypts per mouse) were shown in right panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. Scale bar, 50 μm."
NCBI gene IDs:
"Rest: 19712"
Figure legend: "(D) Immunofluorescence staining of intestine tissues from Srcapfl/fl; Lgr5GFP-CreERT2 and Restfl/fl; Lgr5GFP-CreERT2 mice. Scale bar, 50 μm."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Lgr5: 14160
Rest: 19712
Srcap: 100043597"
Figure legend: "(E) Lgr5GFP-CreERT2;Rosa26lsl-lacZ (LRlacZ) were crossed with Rest-/- mice, followed by TAM administration for lineage tracing for intestinal whole-mount staining for β-gal (upper panel) . Mice were sacrificed after one week and typical sections were shown (middle panel). Scale bar, 200 μm. Numbers of traced crypt-villus units were calculated as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test in right panel. 50 fields (10 fields per mouse) were calculated for each group (n=5) (lower panel)."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Rosa26: 14910
lacZ: 945006
Lgr5: 14160
Rest: 19712"
Figure legend: "(F) Rest+/+ and Rest-/- mice were treated with 10 Gy's radiation and sacrificed at the indicated time points. Intestinal tissues were collected for H&amp;E staining. Scale bars, 100 μm. Numbers of intact crypts were shown in lower panel as means± S.D. ** P &lt; 0.01 by two-tailed Student's t-test. 50 fields (10 fields per mouse) were taken for each group (n=5)."
NCBI gene IDs:
"Rest: 19712"
Figure legend: "(G) Representative images of organoid formation from indicated ISCs were shown (upper panel). Scale bar, 200 μm. n=6 for each group. Organoid number per well was counted as means ± S.D. (lower panel). Lentivirus-carried sgPrdm16 and sgPpard were prepared in 293T cells and infected into ISCs derived from TAM-treated LRCas9 mice to obtain Prdm16-/- and Ppard-/- ISCs. 1×104 ISCs from indicated mice were collected for organoid formation. Scale bar, 200 μm. n=5 for each group."
NCBI gene IDs:
"Cas9: 46806597
Ppard: 19015
Prdm16: 70673"
Figure legend: "f, GFP-LC3-transfected macrophages were infected with wild-type or D actA bacteria. Where indicated, chloramphenicol (CM) was added to the media at 3 h post infection. The number of bacteria per SLAP was quantified. Brackets indicate significant differences, and corresponding P values are shown."
NCBI gene IDs:
"actA: 987035"
Figure legend: "a, GFP-LC3-transfected macrophages were infected for 4 h, and the percentage of infected cells exhibiting SLAPs was quantified. Mean ± s.e.m. for three independent experiments. P values for strains with significant differences from wild-type levels are shown."
NCBI gene IDs:
"LC3: 67443///66734"
Figure legend: "b, Macrophages were infected with IPTG-induced iLLO bacteria. Arrowheads indicate actin- iLLO bacteria within LAMP1+ vacuoles."
NCBI gene IDs:
"LLO: 987033"
Figure legend: "c, Macrophages were infected with wild-type (triangles) or IPTG-induced iLLO (circles) bacteria, and the percentage of LAMP1+ bacteria was quantified. Mean ± s.e.m. for three independent experiments."
NCBI gene IDs:
"LLO: 987033"
Figure legend: "d, Macrophages were infected as in c with or without IPTG induction. Intracellular bacterial replication was determined using a gentamicin-protection assay. Shown is fold replication compared to 2 h post infection. Clear triangles, wild type; filled triangles, wild type + IPTG; clear circles, iLLO; filled circles, iLLO + IPTG. Mean ± s.e.m. or range for three (wild type, wild type + IPTG, iLLO + IPTG) or two (iLLO-IPTG) independent experiments, respectively."
NCBI gene IDs:
"LLO: 987033"
Figure legend: "e, Wild-type or Atg5-/- MEFs were infected with iLLO bacteria, and intracellular bacterial replication was determined as in d. Mean ± s.e.m. for three independent experiments. Clear circles, wild-type MEFs; filled circles, wild-type MEFs + IPTG; clear squares, Atg5-/- MEFs; filled squares, Atg5-/- MEFs + IPTG."
NCBI gene IDs:
"Atg5: 11793
LLO: 987033"
Figure legend: "f, Wild-type (circles) or Atg5-/- (squares) MEFs were infected as in e with IPTG induction. The percentage of LAMP1+ bacteria was quantified as in c. Mean ± s.e.m. for three independent experiments. g"
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "(a) THP-1 cells were transiently infected with BRLF1-expressing or empty control lentivirus. Twenty-four hours later, the control or BRLF1-expressing THP-1 cells were primed with 40 ng/ml TPA overnight and then were either uninfected or infected with HSV-1 (MOI=1) in serum-free medium for 12 h. The supernatants and whole cell lysates were collected and analyzed by western blotting to detect pro-caspase-1 (p45), cleaved caspase-1 (p20), pro-IL-1β, mature IL-1β, pro-IL-18 and mature IL-18 as indicated."
NCBI gene IDs:
"BRLF1: 3783727"
Figure legend: "Ramos cells were infected with wild type EBV-WT or BRLF1-deficient EBV-ΔBRLF1 virus at a high titer (MOI=100). After being transduced with empty control or BZLF1-expressing lentivirus, the supernatants and cell pellets of EBV-WT- or EBV-ΔBRLF1-harboring Ramos cells were collected after 12 h in serum-free culture, and then the levels of EBNA1, BRLF1, BZLF1, pro-caspase-1 (p45), cleaved caspase-1 (p20), pro-IL-1β, mature IL-1β, pro-IL-18, mature IL-18, GSDMD and cleaved N-GSDMD in the supernatants and whole-cell lysates were analyzed as indicated (d)."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744"
Figure legend: "(g) After being transduced with control or BZLF1-expressing lentivirus for 48 h, EBV-WT- and EBV-ΔBRLF1 infected Ramos cells were stained with PI and measured using fluorescent flow cytometry. Representative images of pyroptotic cells are shown and the percentages of pyroptotic cells were calculated in duplicate."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744"
Figure legend: "(a) GST-tagged BRLF1-expressing plasmid or control vector was transfected into A549 cells. After HSV-1 infection (MOI=1) for 12 h, the cells were collected, and the whole cell extracts were subjected to immunoprecipitation with GST affinity beads. The immunoprecipitated complexes were then separated by SDS-PAGE, and the image obtained from silver staining is shown."
NCBI gene IDs:
"GST: 
BRLF1: 3783727"
Figure legend: "(b) Scramble (sc) or shRNAs against POLR3F and POLR3G with empty control vector (ctr) or BRLF1-expressing plasmid were transfected into EBV-ΔBRLF1-harboring HNE1 cells and A549 cells, followed by HSV-1 infection. Thirty-six hours after the transfection of the HNE1 cells, and 12 h after HSV-1 infection (MOI=1) of the A549 cells, the cell pellets were collected and then the cleavage of caspase-1 and IL-1β were measured as indicated. The release of mature IL-1β and IL-18 from HNE-1 and A549 cells were measured by enzyme-linked immunosorbent assay (ELISA) after ultrafiltration and concentration with Amicon® Ultra-0.5 centrifugal filter devices."
NCBI gene IDs:
"BRLF1: 3783727
POLR3F: 10621
POLR3G: 10622"
Figure legend: "(c) GFP-tagged BRLF1 wild type, BRLF1 L578A or empty vector was transfected into 293T cells with GST-tagged POLR3F or POLR3G expressing plasmids for 48 h. Cells were collected and immunoprecipitation with GST-affinity beads was performed, and then whole cell lysates and immunoprecipitated complexes were analyzed as indicated."
NCBI gene IDs:
"GFP: 
BRLF1: 3783727
POLR3F: 10621
POLR3G: 10622"
Figure legend: "(d) GFP-tagged empty vector or GFP-BRLF1- or GFP-BRLF1 L578A-expressing plasmids were transfected into A549 cells, after which the cells were then infected with HSV-1, or transfected into HNE-1-EBV-ΔBRLF1 cells. The cells were then collected and lysed. Immunoprecipitation with GST-affinity beads was subsequently performed, and then whole-cell lysates and immunoprecipitated complexes were analyzed as indicated."
NCBI gene IDs:
"GFP: 
BRLF1: 3783727"
Figure legend: "(a) After infected with wild type EBV-WT or BRLF1-deficient EBV-ΔBRLF1 virus at a high titer (MOI=100), POLR3-dependent RNA transcripts in Ramos cells were detected by RNA-seq analysis. The relative levels of cellular small RNA transcripts are shown as RNA fold changes in the heatmap in the mock (mock group vs. mock group), EBV-WT (EBV-WT group vs. mock group) and EBV-ΔBRLF1 (EBV-ΔBRLF1 group vs. mock group) comparisons."
NCBI gene IDs:
"BRLF1: 3783727
POLR3: 10621///10622"
Figure legend: "(b) EBV-ΔBRLF1-harboring HNE1 cells were transfected with control vector, BRLF1 or BRLF1 L578A in the absence or presence of BZLF1 for 48 h. The total RNAs were extracted and subjected to real-time PCR analysis."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744"
Figure legend: "(c) A549 cells were transfected with control vector, BRLF1 or BRLF1 L578A for 36 h. After the cells were infected with HSV-1(MOI=1) for 12 h, the total RNAs were extracted and subjected to real-time PCR analysis."
NCBI gene IDs:
"BRLF1: 3783727"