EMBO/SourceData huggingface dataset:
Figure legend: "A β-catenin (red) and DDX3 (green) expression in H1299 cells. After overexpressing β-catenin, both DDX3 and β-catenin accumulate in the nucleus. Scale bar is 10 μm."
NCBI gene IDs:
"β-catenin: 1499"
Figure legend: "D, E β-catenin/TCF4 activity was determined by the TOP/FOP reporter assay. Co-transfection with β-catenin is indicated below."
NCBI gene IDs:
"β-catenin: 1499
TCF4: 6925"
Figure legend: "F-I H1299 and A549 cells were treated with RK-33 (0, 1, 2, and 3 μM) and co-transfected with β-catenin in (F, H). Treatment with RK-33 decreased TCF4 activity in both cell lines."
NCBI gene IDs:
"TCF4: 6925"
Figure legend: "J, K Normalized mRNA expression of TCF4-regulated proteins (Axin-2, c-Myc, Cyclin D1) and DDX3 were measured by qRT-PCR in H1299 cells after knockdown of DDX3 (J) and treatment with RK-33 (K). All experiments were repeated three times."
NCBI gene IDs:
"Axin-2: 8313
Cyclin D1: 595
DDX3: 1654
c-Myc: 4609
TCF4: 6925"
Figure legend: "D H1299 cells, containing a stable non-homologous end-joining (NHEJ) reporter construct, were treated with RK-33 and knockdown of DDX3. Reporter construct expressed GFP, which was quantified by flow cytometry. All experiments were repeated three times."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "E Microarray results from MDA-MB-231 cells treated with RK-33 and shDDX3 were validated by qRT-PCR using NHEJ Mechanisms of DSBs Repair PrimePCR plates (Bio-Rad) and performed in biological triplicates."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "(A) GFP time-lapse images of neonatal rat cardiomyocytes expressing EGFP-CLIP-170 WT 0 min and 15 min after treatment with 20 μM Compound C (left side panel) and expressing EGFP-CLIP-170 S311A (right side panel). Higher magniﬁcation of white square (upper row) showing CLIP-170 migrated longitudinally toward the cell-cell junctions."
NCBI gene IDs:
"EGFP: 
CLIP-170: 56430"
Figure legend: "(B) Beeswarm plots of comet length of EGFP-CLIP-170. The comet length from multiple cells in different fields was analyzed. Number of comets analyzed, Pre: n=32, Cpd. C: n=36, CLIP S311A: n=68. Data means ±S.D. Differences among multiple groups were compared by one-way ANOVA, followed by a post hoc comparison using the Tukey method. **, P&lt;0.01 versus Pre."
NCBI gene IDs:
"CLIP: 56430"
Figure legend: "(C) GFP time-lapse images of neonatal rat cardiomyocytes expressing EGFP-CLIP-170 WT treated with control siRNA (siCL, left) or siRNA targeting both AMPKα1 and α2 (siAMPKα1α2, right). White dotted lines in the images showed the connected cardiomyocyte not expressing EGFP-CLIP-170 WT."
NCBI gene IDs:
"AMPKα1: 65248"
Figure legend: "(D) Beeswarm plots of comet length of EGFP-CLIP-170. The comet length from multiple cells from different fields was analyzed. Number of comets analyzed, siCL: n=178, siAMPKα1α2: n=184. Data means ±S.D. Two-tailed Student's t-test was used to analyze differences between two groups. **, P&lt;0.01 versus siCL."
NCBI gene IDs:
"AMPKα1: 65248"
Figure legend: "(C) ﻿Box and whisker plots of the cell size of a cardiomyocyte 2 hours after treatment with or without 2 μM MYK-461, showing the 25th percentile (bottom line of each box), median (middle line of each box), 75th percentile (top line of each box), maximum and minimum (each whisker). Adenovirus expressing EGFP-CLIP-170 (adCLIP) mutant was used (WT, S311A, S311D). Numbers in the graph indicate n number of the cells from 2 independent experiments. Control (-/-): n=784, MYK-461: n=939, CLIP WT: n=656, CLIP WT + MYK-461: n=619, CLIP S311A: n=405, CLIP S311A + MYK-461: n=635, CLIP S311D: n=613, CLIP S311D + MYK-461: n=735. Data means ±S.D. Differences among multiple groups were compared by one-way ANOVA, followed by a post hoc comparison using the Tukey method. **, P&lt;0.01 versus Control, ††, P&lt;0.01 versus CLIP WT, n.s., not significant."
NCBI gene IDs:
"EGFP: 
CLIP: 56430
CLIP-170: 56430"
Figure legend: "(A) Beeswarm plots of the echocardiographic parameter (ejection fraction) of individual Cre control (CLIP-170 S311A +/+; α-MHC-MerCreMer+/−), Control mice (CLIP-170 S311A flox/+; α-MHC-MerCreMer−/−), and CLIP-170 S311A overexpressing mice (CLIP-170 S311A flox/+; α-MHC-MerCreMer+/−), before (Pre), 2 weeks (Tx2w), 8 weeks (Tx8w), 26 weeks (Tx26w) and over 1 year (Tx1y) after tamoxifen induction. Number of mice, (Cre control, Control, S311A), Pre (6, 2, 3), Tx 2w (9, 5, 5), Tx 8w (9, 10, 14), Tx1 2w (7, 10, 14), Tx 26w (3, 7, 8), Tx 52w (3, 7, 9)."
NCBI gene IDs:
"CLIP-170: 56430
Cre: 2777477
Mer: 17289
α-MHC: 17888"
Figure legend: "(B) Beeswarm plots of another line of CLIP-170 S311A overexpressing mice (line 9). Number of mice, (Control, S311A), Pre (7, 3), Tx 2w (4, 7), Tx 8w (6, 4), Tx 12w (5, 4), Tx 52w (3, 3). Data means ±S.D. Statistical significance was determined by two-way ANOVA followed by post hoc Tukey's multiple comparison test. ##: p &lt; 0.01 vs Duration-matched Cre Control, *: p &lt; 0.05, **: p &lt; 0.01 vs Duration-matched Control; †: p &lt; 0.05, ††: p &lt; 0.01, S311A TG mice 12 weeks vs 52 weeks."
NCBI gene IDs:
"CLIP-170: 56430
Cre: 2777477"
Figure legend: "(C) Representative long axis four-chamber cardiac magnetic resonance images of CLIP-170 S311A TG mice and control mice over 1 year after tamoxifen induction. Left column showed systole images and right column represented diastole images. Three mice from each group were analyzed for cardiac MRI. The representative images were shown."
NCBI gene IDs:
"CLIP-170: 56430"
Figure legend: "(E) Beeswarm plots of the echocardiographic parameter (ejection fraction) from CLIP-170 S311D overexpressing mice and the control. Number of mice, (Control, S311D), Tx 2w (3, 4), Tx 8w (5, 11), Tx 26w (4, 11). Data means ±S.D. There was no significant difference."
NCBI gene IDs:
"CLIP-170: 56430"
Figure legend: "(A) Masson's trichrome staining of the heart of CLIP-170 S311A overexpressing mice and the control mice from 3 months and 1 year after tamoxifen treatment."
NCBI gene IDs:
"CLIP-170: 56430"
Figure legend: "(C) Representative immunostained images with an α-tubulin antibody of CLIP-170 S311A overexpressing mice heart and control mice heart over 1 year after tamoxifen treatment. (D) Quantitative analysis of intensity of α-tubulin in (C). Number of mice, (Control: n=7, S311A: n=8). Average of 5 sections per animal were analyzed. **, P&lt;0.01 versus Control. "
NCBI gene IDs:
"CLIP-170: 56430"
Figure legend: "(G) Representative immunostained images of CLIP-170 S311A overexpressing mice heart and the control mice heart over 1 year after tamoxifen treatment. These were stained with WGA (red) and a plakoglobin antibody (green)."
NCBI gene IDs:
"CLIP-170: 56430"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression and BMI Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression , plasma alanine aminotransferase (ALAT) (B) Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression and liver fat content (C). Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "ASK1 mRNA expression in patients with different NASH scores Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D). ***"
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression and ATG5 (E) mRNA expression. Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D). ***"
NCBI gene IDs:
"ATG5: 9474
ASK1: 4217"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression and ATG7 Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D)."
NCBI gene IDs:
"ATG7: 10533
ASK1: 4217"
Figure legend: "Scatter plot and correlation coefficient (r) of ASK1 mRNA expression and ATG12 mRNA expression. Data were collected in lean subjects (n=14), obese subjects (n=23) and obese subjects with type 2 diabetes (n=19). Values are expressed as mean ± SEM (D). ***"
NCBI gene IDs:
"ATG12: 9140
ASK1: 4217"
Figure legend: "Shown is one representative Western blot from two independent experiments of hepatocytes transfected with siRNA targeting ASK1 (siASK1) or non-targeting siRNA control (siCtrl)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Lipid accumulation was quantified using automated image-based analysis in ASK1 knockdown (siASK1; grey bars) or control (siCtrl; black bars) cells treated with BSA, Palm, Palm+Rap and stained as mentioned above (n=4 biological replicates)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Shown is one representative Western blot from two independent experiments and quantification of LC3-II/I and p62 protein levels in siCtrl (n=4 biological replicates) or siASK1 (n=4 biological replicates) cells treated with Palm."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Colocalization of LC3-II punctate (red; arrow) with lipid (BODIPY 493/503, green) and nuclei (Hoechst, blue) in siCtrl or siASK1 cells treated with BSA, Palm or Palm+Rap. Scale bar represents 100 µm."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Colocalization of LC3-II punctate with lipid droplets (BODIPY 493/503, green) was quantified in hepatocytes transfected with siRNA targeting ASK1 (siASK1; grey bars) or non-targeting siRNA control (siCtrl; black bars) and treated with BSA or BSA+Baf for 24 hours (n=4 biological replicates)."
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Lipid accumulation was quantified using automated image-based analysis in ASK1 knockdown (siASK1; grey bars) or control (siCtrl; black bars) cells treated with BSA, Palm or Palm+Baf and stained as mentioned above (n=4 biological replicates). Values are expressed as mean ± SEM. *"
NCBI gene IDs:
"ASK1: 4217"
Figure legend: "Liver triglyceride (TG) content of mice fed a chow (ASK1F/F n=6 mice; ASK1Δhep n=6 mice) or HFD (ASK1F/F n=14 mice; ASK1Δhep n=15 mice)."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Relative mRNA expression of perilipin (Plin) and very low-density lipoprotein receptor (Vldlr) in liver of mice fed a HFD for 20 weeks (ASK1F/F n=6 mice; ASK1Δhep n=7 mice (Vldlr) or n=8 mice (Plin))."
NCBI gene IDs:
"ASK1: 26408
perilipin: 103968
Plin: 103968
very low-density lipoprotein receptor: 22359
Vldlr: 22359"
Figure legend: "Relative mRNA expression of genes involved in inflammation in liver of mice fed a HFD for 20 weeks (ASK1F/F n=6 mice; ASK1Δhep n=7 mice (F4/80, Mcp1) or ASK1Δhep n=8 mice (Il-6))."
NCBI gene IDs:
"F4/80: 13733
Mcp1: 20296
Il-6: 16193
ASK1: 26408"
Figure legend: "Relative mRNA expression of collagen, type I, alpha 1 (Col1a1) (ASK1F/F n=11 mice; ASK1Δhep n=15 mice) and transforming growth factor beta 1 (Tgfβ1) (ASK1F/F n=6 mice; ASK1Δhep n=8 mice) in liver of HFD-fed mice."
NCBI gene IDs:
"Col1a1: 12842
collagen, type I, alpha 1: 12842
ASK1: 26408
Tgfβ1: 21803
transforming growth factor beta 1: 21803"
Figure legend: "quantification of Sirius Red positive area in liver of mice fed a HFD (ASK1F/F n=8 mice; ASK1Δhep n=8 mice)."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Shown is one representative Western blot from two independent experiments and quantification of hepatic COL1A1 protein levels in mice fed a HFD for 20 weeks (ASK1F/F n=8 mice; ASK1Δhep n=8 mice)."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Liver triglyceride (TG) content (ASK1F/F n=7 mice; ASK1Δhep n=7 mice) and quantification of Sirius Red positive area in livers (ASK1F/F n=7 mice; ASK1Δhep n=7 mice) of 15 months old chow-fed mice. Values are expressed as mean ± SEM. #"
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Shown is one representative Western blots from two independent experiments and quantification of respective protein levels in mice fed a HFD for 20 weeks (ASK1F/F n=8 mice; ASK1Δhep n=8 mice)."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Primary hepatocytes isolated from chow-fed ASK1F/F () and ASK1Δhep () mice were treated with BSA, Palm, Palm+Rap or Palm+Rap+Baf for 24 h. Cells were stained for lipid droplet accumulation (Bodipy 493/503, green) and nuclei (Hoechst, blue). Lipid accumulation was quantified using automated image-based analysis (n=4 biological replicates)."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Liver triglyceride (TG) content (ASK1F/F n=11 mice; ASK1+hep n=10 mice) in livers of mice fed a HFD for 20 weeks."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Quantification of Sirius Red positive area (ASK1F/F n=11 mice; ASK1+hep n=9 mice) in livers of CCl4-injected mice."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Relative mRNA expression (ASK1F/F n=13 mice; ASK1+hep n=14 mice) of respective genes involved in inflammation and fibrosis in liver of CCl4-injected mice. Values are expressed as mean ± SEM. #"
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "Relative mRNA expression (ASK1F/F n=13 mice; ASK1+hep n=14 mice) of respective genes involved in inflammation and fibrosis in liver of CCl4-injected mice. Values are expressed as mean ± SEM."
NCBI gene IDs:
"ASK1: 26408"
Figure legend: "C. Imaging of  SPBs  (Cnm67-RFP) and Spc72-GFP  or  Mpc70-GFP in control and PHSL1-CDC20 cells. Top, time-lapse series. Bottom, meiotic events were quantified in cells synchronized  in silico  to SPB separation at entry into metaphase I  (t = 0). Graphs show overlays of SPC72-GFP and MPC70-GFP strains. Data information: data are representative of four independent experiments."
NCBI gene IDs:
"CDC20: 852762
HSL1: 853760"
Figure legend: "D. Imaging of SPBs (Cnm67-RFP) and Spc72-GFP or  Mpc70-GFP in PHSL1-CDC20  strains containing the indicated mutations. Meiotic events were analyzed as in (C). Data information: data are representative of two independent experiments."
NCBI gene IDs:
"CDC20: 852762
HSL1: 853760"
Figure legend: "A. Imaging of  SPBs (Cnm67-RFP) and Spc72-GFP or Mpc70-GFP in PHSL1-CDC20 ama1∆ control cells and spo13  mutants. Top, time-lapse series. Bottom, meiotic  events were quantified in cells synchronized  in silico to SPB  separation at entry into metaphase I (t = 0). Graphs show overlays of SPC72-GFP and MPC70-GFP strains. Data information: data are representative of two independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
HSL1: 853760
spo13: 856405"
Figure legend: "C. Imaging of SPBs (Cnm67-RFP) and Spc72-GFP  or Mpc70-GFP in PHSL1-CDC20 ama1∆ control cells and cells containing clb1∆ or cdc28-as1, which have been treated with 1NM-PP1 at metaphase I (8 h in SPM). Meiotic events (bottom) were analyzed as in (A). Data information: data are representative of two independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
clb1: 853002
HSL1: 853760"
Figure legend: "A. Imaging of  SPBs  (Cnm67-RFP) and Mpc70-GFP in PHSL1-CDC20 ama1∆ cells containing SPC72, spc72-7, or spc72-7 plus     hrr25-as. Cells were shifted from 24 to 36°C at 4 h in  SPM and treated with 1NM-PP1 to inhibit  Hrr25-as. Top, time-lapse series. Bottom,  Mpc70 loading to SPBs was quantified in cells synchronized  in silico to SPB  separation at entry into  metaphase I  (t = 0). Data information: data are representative of three independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
hrr25: 855897
HSL1: 853760
SPC72: 851250
spc72: 851250"
Figure legend: "C. Imaging of  SPBs (Cnm67-RFP) and Mpc70-GFP in PHSL1-CDC20 ama1∆  spo13∆ control cells and cells expressing an additional copy of SPC72 from the PEST promoter at 4 h in SPM. Mpc70  loading (bottom) was analyzed as in (A). PEST-SPC72 expression delays the  time of Mpc70 loading by 98 min (95%  CI, 67-129; P &lt; 0.0001; Welch's t-test). Data information: data are representative of three independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
HSL1: 853760
SPC72: 851250
spo13: 856405"
Figure legend: "(B) Analysis of cells containing wild-type   Spo13, Spo13-mD, or Spo13-mD-m2.  Meiotic events  were quantified in cells synchronized  in silico to SPB reduplication at entry into metaphase II  (t = 0). Graphs show overlays of SPC72-GFP and  MPC70-GFP strains.  spo13-mD delays Spc72 removal and Mpc70  loading by 30 min (95%  CI, 26-34; P &lt; 0.0001) and 60 min (95% CI, 55-66; P &lt; 0.0001; Welch's t-test), respectively. (C) Analysis of cells expressing Clb1-mDK and different versions of  Spo13. Meiotic events were quantified as in (B). spo13-mD plus PEST-clb1-mDK delays Spc72 removal and  Mpc70  loading by 72 min (95% CI, 63-80; P &lt; 0.0001) and 114 min (95%  CI, 99-129; P &lt; 0.0001; Welch's t-test), respectively."
NCBI gene IDs:
"Clb1: 853002
clb1: 853002
spo13: 856405
Spo13: 856405"
Figure legend: "D.  Cdc5's polo-box domain (PBD)  binds to Ime2. Ha3-tagged versions of the wild-type PBD or the phosphopeptide-binding mutant PBD-FAA were expressed at t = 5.5 h in SPM in cells depleted of Cdc20  and Cdc5. PBD-ha3  and Ime2 were detected by immunoblotting in whole-cell extracts and α-Ha immunoprecipitates. Data information: data are representative of two independent experiments."
NCBI gene IDs:
"Cdc20: 852762
Cdc5: 855013"
Figure legend: "E.  Cdc5-dependent modification of Ime2. PHSL1-CDC20 ama1∆ control cells and cells containing  spo13∆ or  cdc5-as were treated with CMK (to inhibit Cdc5-as) at t = 8 h in SPM (arrow heads). The panel shows  immunoblot analysis of whole-cell extracts. C, sample from proliferating cells. Increased gel mobility of Ndt80 at t ≥ 10 h confirms inhibition of Cdc5-as. The arrow marks the modified form of  Ime2. Data information: data are representative of two independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
cdc5: 855013
Cdc5: 855013
HSL1: 853760
spo13: 856405"
Figure legend: "A, B. Mitotic cdc20-3 cells were shifted to 36°C for 80 min. At t = 0, cells were treated with  estradiol  to induce expression from the PEST promoter. Top, time-lapse series from the imaging of SPBs (Cnm67-RFP) and Spc72-GFP. Frame width, 19 μm. Bottom, quantification of cells with  Spc72-GFP at SPBs. (A) Expression  of  Ime2-∆C induces removal of Spc72 from SPBs, which is inhibited by co-expression of Spo13. (B) Expression of Cdc5  plus Ime2-∆C causes rapid removal of Spc72, which is inhibited by co-expression of Spo13. Data information: data are representative of three independent experiments."
NCBI gene IDs:
"cdc20: 852762
Cdc5: 855013
Ime2: 853338
Spo13: 856405"
Figure legend: "C. Mitotic cdc20-3 cells containing PEST-CDC5 plus PEST-IME2-∆C and/or PEST-NDT80 were shifted to 36°C for 30 min and subsequently treated with  estradiol (t = 0). Top, time-lapse  series from the imaging of SPBs  (Cnm67-RFP) and Mpc70-GFP. Frame width, 19 μm. Bottom, quantification of cells with Mpc70-GFP at SPBs. Data information: data are representative of two independent experiments."
NCBI gene IDs:
"cdc20: 852762
CDC5: 855013
IME2: 853338
NDT80: 856524"
Figure legend: "C. PHSL1-CDC20 ama1∆ cdc28-as2 control cells and cells containing  spo13∆ or  cdc5-as were treated with 1Na-PP1 at t = 3 h in  SPM to inhibit Cdc28-as2. At t = 9 h in  SPM, Cdc28-as2 was activated by washing cells with cSPM lacking 1Na-PP1 (washout). cdc5-as cells were washed with cSPM plus  CMK to activate Cdc28-as2 and inhibit  Cdc5-as. Top,  time-lapse series from the imaging of Mpc70-GFP and RFP-tubulin. The weak, nuclear signal originates from Ndt80-GFP. Bottom, quantification of  spindle  formation and Mpc70  loading to  SPBs. D. PHSL1-CDC20 ama1∆ cells containing SPC72, spc72-7, or spc72-7 plus cdc5-as were shifted from 24 to 36°C at t = 4.2 h in  SPM (to inactivate Spc72-7, arrow heads) and treated with CMK at t = 6 h in SPM (to inhibit Cdc5-as). Top, time-lapse  series from the imaging of SPBs (Cnm67-RFP) and Mpc70-GFP. Bottom, quantification of SPB separation and Mpc70 loading to  SPBs. Inhibition of  Cdc5-as in spc72-7 cells advances the  time of Mpc70  loading by 119 min (95% CI, 76-162; P &lt; 0.0001; Welch's t-test). Data are representative of two independent experiments."
NCBI gene IDs:
"ama1: 853140
CDC20: 852762
cdc28: 852457
Cdc28: 852457
cdc5: 855013
Cdc5: 855013
HSL1: 853760
SPC72: 851250
spc72: 851250
Spc72: 851250
spo13: 856405"
Figure legend: "A. Inhibition of Cdk1 at anaphase I. CDC20-mAR and CDC20-mAR cdc28-as1 cells were released from the metaphase I-arrest with CuSO4 at 7 h in SPM  (t = 0) and treated with 1NM-PP1  at t = 40 min to inhibit Cdc28-as1 (arrows). Top, time-lapse series from the imaging of Mpc70-GFP, SPBs (Cnm67-RFP), and nuclei  (TetR-RFP). Bottom, quantification of meiotic events."
NCBI gene IDs:
"CDC20: 852762
cdc28: 852457"
Figure legend: "Western blotting of Myc-Egg expressed in OSCs. Anti-Myc (Myc) and anti-β-Tubulin (Tub) antibodies were used. Tub was detected as a loading control. Control: empty vector was used as a negative control."
NCBI gene IDs:
"Myc: 
Egg: 37962"
Figure legend: "Western blotting of Myc-Egg WT and its K1085R and S215/T217 (STAA) mutants expressed in OSCs. Egg modifications identified in this study are summarized on the right. P: phosphorylation. Ub: monoubiquitination."
NCBI gene IDs:
"Myc: 
Egg: 37962"
Figure legend: "Rescue assays. Myc-Egg WT and its K1085R and STAA mutants were expressed in OSCs upon endogenous Egg depletion (siEgg). Western blotting (WB) was performed using anti-Egg and anti-Myc antibodies. RT-qPCR was used to quantify Stalker2 transposon expression level. The act5c gene was used for normalization. siEGFP: siRNA for EGFP was used as a negative control. Error bars represent SD from three independent experiments. *p &lt; 0.05 (unpaired Student's t-test). n.s.: not significant."
NCBI gene IDs:
"act5c: 
EGFP: 
Myc: 
Stalker2: 
Egg: 37962"
Figure legend: "Western blotting of Myc-Egg expressed in OSCs before (siEGFP) and after (siUbc2) Ubc2 depletion. Anti-Myc-antibody was used for western blotting. The ratios of mUb-Egg over non-mUb-Egg band signals were 1.43 (siEGFP) and 0.78 (siUbc2)."
NCBI gene IDs:
"EGFP: 
Ubc2: 34487"
Figure legend: "Immunofluorescence analysis of Myc-Egg WT, Myc-SV40-NLS-Egg, and Myc-PKI-NES-Egg expressed in OSCs using anti-Myc antibody. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Myc: 
Egg: 37962"
Figure legend: "Western blotting of Myc-Egg WT, Myc-SV40-NLS-Egg, and Myc-PKI-NES-Egg expressed in OSCs. Anti-Myc antibody was used. The ratios of mUb-Egg over non-mUb-Egg band signals were 1.50 (WT), 2.29 (SV40-NLS), and 0.95 (PKI-NES)."
NCBI gene IDs:
"Myc: 
Egg: 37962"
Figure legend: "Rescue assays. Myc-Egg WT, Myc-SV40-NLS-Egg, and Myc-PKI-NES-Egg were expressed in OSCs upon endogenous Egg depletion (siEgg). Western blotting was performed using anti-Egg (left) and anti-Myc (right) antibodies. Stalker2 transposon expression was quantified using RT-qPCR. The act5c gene was used for normalization. siEGFP: siRNA for EGFP was used as a negative control. Error bars represent SD from three independent experiments. *p &lt; 0.05 (unpaired Student's t-test)."
NCBI gene IDs:
"act5c: 
Stalker2: 
EGFP: 
Myc: 
Egg: 37962"
Figure legend: "Rescue assays. Myc-Egg WT, Myc-Egg K1085R, and Myc-SV40-NLS-K1085R were expressed in OSCs upon endogenous Egg depletion (siEgg). Western blotting was performed using anti-Egg (left) and anti-Myc (right) antibodies. RT-qPCR quantified the expression level of the Stalker2 transposon. The act5c gene was used for normalization. siEGFP: siRNA for EGFP was used as a negative control. Error bars represent SD from three independent experiments. *p &lt; 0.05 (unpaired Student's t-test)."
NCBI gene IDs:
"act5c: 
Stalker2: 
EGFP: 
Myc: 
Egg: 37962"
Figure legend: "Immunofluorescence analysis of endogenous Egg (red) in OSCs using anti-Egg antibody before (siEGFP) and after (siWde) Wde depletion. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"EGFP: 
Wde: 36133"