EMBO/SourceData huggingface dataset:
Figure legend: "(A) Gene expression level of stromal activation markers COL1A1, ACTA2, SPARC, FN1 and FAP in PS-1 cells which were exposed for 72 hrs to CM from control (CM of PS-1 cells ), epithelial (blue) or mesenchymal (red) PDAC cell lines using qPCR. Values were normalized to control and n=6 per group, 3 biological replicates for each cell line. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"ACTA2: 59
COL1A1: 1277
FAP: 2191
FN1: 2335
SPARC: 6678"
Figure legend: "(B) Heatmap representation of COL1A1 and ACTA2 relative gene expression levels measured with qPCR in PS-1 cells after treatment using a large cohort of PDAC cell lines and primary cell cultures 67 and 53m. Values were Log2 transformed and normalized to Suit-2 expression. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"ACTA2: 59
COL1A1: 1277"
Figure legend: "(D) Gene expression levels of COL1A1 and ACTA2 in PS-1 cells after treatment using qPCR, values were normalized to control. n=3 technical replicates per group. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"ACTA2: 59
COL1A1: 1277"
Figure legend: "(F) Relative gene expression levels of COL1A1 and ACTA2 in primary CAFs after treatment using qPCR, values were normalized to control. n=3 technical replicates per group. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"ACTA2: 59
COL1A1: 1277"
Figure legend: "(B) Gene expression levels of COL1A1 and ACTA2 in PS-1 cells after treatment using qPCR. Data was normalized against control CM. Statistical significance was tested against unfiltered CM samples. Graph shows n=3 technical replicates of an example experiment that was performed three times. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"ACTA2: 59
COL1A1: 1277"
Figure legend: "(E) Gene expression levels of COL1A1 in PS-1 cells after treatment using qPCR. n=3 biological replicates per group. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"COL1A1: 1277"
Figure legend: "(B) Gene expression levels of COL1A1 in PS-1 cells after treatment using qPCR. Graph shows n=3 technical replicates of an example experiment that was performed three times. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"COL1A1: 1277"
Figure legend: "(C) Brightfield images of PS-1 cells after 72 hrs of treatment with PANC-1 CM of pLKOctrl or shCSF1 (#560-562). Scale bar represents 100 µm."
NCBI gene IDs:
"CSF1: 1435"
Figure legend: "(D) Correlation graph of CSF1 expression in PANC-1 pLKOctrl or shCSF1 (#560-562) and how these various CM affect COL1A1 expression in PS-1 cells using qPCR, values were normalized for RPLPO expression. n=3 biological replicates for all individual experiments, error bars indicated SD. R2 and P-value were analyzed with linear regression."
NCBI gene IDs:
"COL1A1: 1277
CSF1: 1435
RPLPO: 6175"
Figure legend: "(E) Brightfield images of PS-1 cells after 72 hrs of treatment with CM of Capan-2 cells with control vector or Capan-2 cells overexpressing CSF1. Scale bar represents 100 µm."
NCBI gene IDs:
"CSF1: 1435"
Figure legend: "(F) Gene expression level of CSF1 in Capan-2 cells after transduction with control vector or overexpressing CSF1. n=3 technical replicates per group. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"CSF1: 1435"
Figure legend: "(G) Gene expression levels of COL1A1 in PS-1 cells after treatment indicated using qPCR. Graph shows n=3 technical replicates of an example experiment that was performed three times. Data information: data are represented as mean±SD, Student's t-test."
NCBI gene IDs:
"COL1A1: 1277"
Figure legend: " Fitting of time courses (filled circles) of the translation of LepB mRNA constructs of increasing length (Appendix Fig S3) by delay-exponential functions (red lines). "
NCBI gene IDs:
"LepB: 947040"
Figure legend: " Stopped-flow time courses for translocon insertion of TM1 of EmrD (black) and EmrD(-) (blue) with mRNA constructs of varying chain length (50 to 135 aa).The FRET acceptor (Atto655) was placed at the N-terminus of the nascent peptide, the donor (Atto488) at position 111 (donor-in) of SecY. The results of global fitting are indicated (red lines). "
NCBI gene IDs:
"EmrD: 948180"
Figure legend: "(A) Schematic showing the tamoxifen  injection paradigm for tdTomato-labelling of all IFE  lineages in tail  and back. Co-expression of markers with the lineage-traced cells at 1-month post-tamoxifen. Dlx1-CrER  is shown as example in tail; Scale bar =50 µm (B) Quantification of images like those in (A) showing % tdTomato+ cells expressing indicated markers. Sox6 (black bar) was not analyzed in K14-Cre/EYFP  lineage. Error bars are SDs. P-values calculated by Student's t-test (mixed effects model) from n=2-3 mice  and 5-6 images per replicate."
NCBI gene IDs:
"EYFP: 
tdTomato: 
Cr: 2777477
Cre: 2777477
Dlx1: 13390
ER: 2099
K14: 16664"
Figure legend: "(G) Immunofluorescence  images of Sox6 staining  in noUVB (0hr) or UVB-exposed (6hrs- or 24hrs-post) mouse back skin. (H) Boxplot shows % Sox6  expressing  basal cells  quantified from images like those in (G). Central band is the mean value, the top of box indicates 25th percentile while the bottom indicates 75th percentile. Whiskers indicate minimum and maximum values in the data, with dots indicating potential outliers. Linear mixed model statistical test was used with data from 18 images from 3 biological replicates."
NCBI gene IDs:
"Sox6: 20679"
Figure legend: "A Representative fluorescent images of centrosomes  in RPE1  WT, CEP250  KO and CROCC  KO cells, without and with MT  depolymerization using Nocodazole. PCNT  was used to mark centrosomes  and DNA was stained  with DAPI. The boxes in the right-hand corner show enlargement of the centrosome  signals of the cell in the main panel. White scale bars, 5 μm. Yellow scale bars, 1 μm. B Quantification of cells with separated centrosomes (centrosome  distance &gt; 2 μm) from RPE1  samples (A). N = 50 cells per experiment, n = 3 independent experiments. For WT with Nocodazole, N = 50 cells, n = 2 independent experiments. Bar and error represent mean and SD. Unpaired t-tests. n.s.: not significant p &gt; 0.05, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"CEP250: 11190
CROCC: 9696"
Figure legend: "C Representative fluorescent images of centrosomes  in HCT116  WT, CEP250  KO, and CROCC  KO, without and with MT  depolymerization using Nocodazole. γ-tubulin was used to mark centrosomes  and DNA was stained  with DAPI. The boxes in the right-hand corner show enlargements of the centrosome  signals of the cell in the main panel. White scale bars, 10 μm. Yellow scale bars, 2 μm. D Quantification of cells with separated centrosomes  (centrosome  distance &gt; 1 μm) from HCT116  samples (C). N = 50 cells per experiment, n = 3 independent experiments. Bar and error represent mean and SD. Unpaired t-tests. ** p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001."
NCBI gene IDs:
"CEP250: 11190
CROCC: 9696"
Figure legend: "A-H (A, C, E, G)  Time-lapse images (4 min interval) of RPE1  WT, CEP250  KO, NIN  KO and CROCC  KO cells expressing  γ-tubulin-mRuby2  (red) as marker for the centrosomes  with and without Nocodazole  treatment. Representative still images (top rows) and kymographs  of centrosomes  (bottom rows) are shown. The DNA was marked with SPY-DNA650 (grey). White scale bars, 5 μm. Yellow scale bars, 2 μm. (B, D, F, H) Intercentrosomal distances (μm) from (A), (C), (E) and (G) were plotted over time  (n = 15 cells)."
NCBI gene IDs:
"CEP250: 11190
CROCC: 9696
NIN: 51199"
Figure legend: "A Indirect immunofluorescence  analysis of Ninein  at the centrosomes  in RPE1  WT, CEP250  KO and CEP128  KO cells. CEP164  marks the mother centriole, γ-tubulin  was used as centrosome  marker. The model panel illustrates the position of the centrioles  and  centriole appendages. Scale bars: 1 μm. B STED super resolution image and model of Ninein  at the centrosome  in RPE1  WT, CEP250  KO and CEP128  KO cells. CEP164, C-Nap1  and GT335   were used to mark the mother centriole, the proximal end of centrioles, and the centriole wall, respectively. The model panel illustrates the position of the centrioles  and appendages  (colours as indicated). For WT and CEP250  KO a blue centriole  model was used for better illustration of the different positions. Scale bars: 1 μm."
NCBI gene IDs:
"CEP128: 145508
CEP250: 11190"
Figure legend: "E-H (E, G) Fluorescent images of centrosomes  in RPE1 CEP250  KO and CEP128  KO cells with the indicated siRNA depletions, without and with MT  depolymerization using Nocodazole. PCNT  or γ-tubulin was used to mark centrosomes  and DNA was stained  with DAPI. The box in the right-hand corner shows the centrosome  signals of the cell in the main panel. Scale bars in white: 5 μm. Scale bars in yellow: 1 μm. (F, H) Quantification of cells with separated centrosomes (centrosome  distance &gt; 2 μm) for RPE1 CEP250  KO (E) and CEP128  KO (G) samples. N = 50 cells per experiment, n = 3 independent experiments. Bar and error represent mean and SD. Unpaired t-test. n.s.: not significant p &gt; 0.05, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"CEP128: 145508
CEP250: 11190"
Figure legend: "C Representative fluorescent images of RPE1 CEP250  KO and CEP250  KO expressing C-Nap1-HA-mNeonGreen  (CEP250  KO + CEP250). The indicated antibodies were used for indirect fluorescent analysis. Scale bars in white: 5 μm; scale bars in yellow: 1 μm. D Quantification of centrosome  separation from (C). N = 50 cells per experiment, n = 3 independent experiments. Bar and error represent mean and SD Unpaired t-test. n.s.: not significant p &gt; 0.05, **** p &lt; 0.0001. E Quantification of the average number (left) and length (right) of MTs  re-nucleated from the centrosomes  in (A) from RPE1  WT and CEP250  KO cells with PLK4   OE. N = 50 cells per experiment, n = 3 independent experiments. Bar and error represent mean and SD Unpaired t-test. ** p &lt; 0.01."
NCBI gene IDs:
"C-Nap1: 
HA: 
mNeonGreen: 
CEP250: 11190
PLK4: 10733"
Figure legend: "B Fluorescent images of centrosomes  in Dox-inducible Flag-PLK4 RPE1  WT, CEP250  KO, NIN  KO and CROCC  KO cells, with and without Dox  induction. Flag-PLK4  was stained  with Flag  antibodies, PCNT  was used as centrosome  marker and DNA was stained with DAPI. The boxes on top show enlargement of the centrosome  signals of the cell in the main panel. Scale bars in white: 5 μm; scale bars in yellow: 1 μm."
NCBI gene IDs:
"Flag: 
CEP250: 11190
CROCC: 9696
NIN: 51199
PLK4: 10733"
Figure legend: "D Fluorescent images of centrosomes  in Dox-inducible Flag-PLK4 RPE1  WT, CEP250  KO, NIN  KO and CROCC  KO cells upon 48 h Dox  induction, without and with MT  depolymerization using Nocodazole. DNA was stained  with DAPI. Scale bars: 5 μm. E Quantification of the diameter of amplified centrosomes  from (D) as outlined in (A). A representative dataset from six replicates (three replicates per experiment) is shown. N = 50 cells. Dot represents the diameter of amplified centrosomes  in each cell with amplified centrosomes. The red lines represent the median. Unpaired t-test. n.s.: not significant p &gt; 0.05, * p &lt; 0.05, ** p &lt; 0.01, **** p &lt; 0.0001."
NCBI gene IDs:
"Flag: 
CEP250: 11190
CROCC: 9696
NIN: 51199
PLK4: 10733"
Figure legend: "A Representative images of live cell imaging  analysis of RPE1  WT, CEP250  KO, WT PLK4  OE and CEP250  KO PLK4  OE cells expressing LaminB1-mNeonGreen  (magenta) and  γ-tubulin-mRuby2  (green) as markers for the NE  and centrosomes, respectively. Yellow arrows indicate first NE fold. White arrows indicate first NE  discontinuation. The inlet boxes show enlargement of first NE  discontinuation. Scale bars: 5 μm."
NCBI gene IDs:
"CEP250: 11190
PLK4: 10733"
Figure legend: "C Quantification of NEBD  preparation time. WT: n = 54 cells from 6 independent experiments, CEP250  KO: n = 56 cells from 6 independent experiments, WT PLK4  OE: n = 48 cells from 7 independent experiments, CEP250  KO PLK4  OE: n = 47 cells from 8 independent experiments. D Quantification of NEBD  duration. WT: n = 54 cells from 6 independent experiments, CEP250  KO: n = 56 cells from 6 independent experiments, WT PLK4  OE: n = 48 cells from 7 independent experiments, CEP250  KO PLK4  OE: n = 47 cells from 8 independent experiments."
NCBI gene IDs:
"CEP250: 11190
PLK4: 10733"
Figure legend: "H Representative images from live cell imaging  analysis of WT, CEP250  KO, WT PLK4  OE and CEP250  KO PLK4  OE cells expressing γ-tubulin-mRuby2  (red) as a marker for the centrosomes. The DNA was marked by  SPY-DNA650 dye (cyan). Gray arrows highlight chromosome  segregation errors. Scale bars: 5 μm."
NCBI gene IDs:
"CEP250: 11190
PLK4: 10733"
Figure legend: "I Fluorescent images of RPE1  WT, CEP250  KO, WT PLK4  OE and CEP250  KO PLK4  OE cells. BUB1  and MAD1  were stained   to analyse the signal distribution or accumulation/persistence. CENP-C was used as centromere  marker, α-tubulin  to visualize the mitotic spindle and DNA was stained  with DAPI. Scale bars: 5 μm."
NCBI gene IDs:
"CEP250: 11190
PLK4: 10733"
Figure legend: "A Fluorescent image of mitotic spindles in Dox-inducible Flag-PLK4 RPE1  WT, CEP250  KO, NIN  KO and CROCC  KO cells upon 48 h Dox  induction (PLK4   OE). CW069  was used to partially inhibit HSET. DMSO  is solvent control. White scale bars: 5 μm. B Quantification of mitotic cells with  multipolar spindle from (A). N = 50 cells per experiment, n = 3 independent experiments. Bar and error represent mean and SD. Unpaired t-test. * p &lt; 0.05, ** p &lt; 0.01, *** p &lt; 0.001. C Quantification of  multipolar spindle/chromosome  mis-segregation of Dox-inducible Flag-PLK4 RPE1  WT and CEP250  KO cells upon 48 h Dox  induction expressing γ-tubulin-mScarlet-I (red) as markers for the centrosomes with or without CW069  treatment. The DNA was marked with SPY-DNA650 (grey). WT PLK4   OE: nDMSO = 28 cells, nCW069 = 40 cells, CEP250  KO PLK4   OE: nDMSO = 26 cells, nCW069 = 31 cells; from 2 independent live cell experiments. Bar represents mean. Unpaired t-test. * p &lt; 0.05."
NCBI gene IDs:
"Flag: 
CEP250: 11190
CROCC: 9696
NIN: 51199
PLK4: 10733"
Figure legend: "D Time-lapse  analysis of RPE1  WT PLK4  OE and CEP250  KO PLK4   OE cells expressing γ-tubulin-mScarlet-I (red) as markers for the centrosomes followed by 30 h siHSET. The DNA was marked with SPY-DNA650 (grey). (D) shows representative still images of live cell imaging  analysis. Scale bars: 10 μm. E Quantification of multipolar spindle/chromosome  mis-segregation from (D) in RPE1  WT PLK4   OE and CEP250  KO PLK4   OE with siControl and siHSET for 30 h. WT PLK4   OE: nsiControl = 59 cells, nsiHSET = 59 cells, CEP250  KO PLK4  OE: nsiControl = 45 cells, nsiHSET = 32 cells; from 3 independent experiments. Bar and error represent mean and SD. Unpaired t-test. * p &lt; 0.05, ** p &lt; 0.01."
NCBI gene IDs:
"CEP250: 11190
HSET: 3833
PLK4: 10733"
Figure legend: "(B) Quantification of free cholesterol levels in total homogenates of APP-DKO cells transiently expressing C99, C83 or AICD peptides, or treated with 5 µM Aβ42 oligomers for 16h. Cholesterol levels of WT cells are shown as a control. Lipid units are represented as molar mass over total moles of lipids analyzed (mol %). Graphs represent fold change over controls. One-way ANOVA (n = 4; * p&lt;0.05)."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(A) Crude membrane fractions from APP-DKO cells expressing C99WT or C99MUT were treated with 0.2% Triton-X-100, loaded onto continuous density sucrose gradients and centrifuged for 16h. Fractions from these gradients were analyzed by western blot to determine the migration of the indicated proteins [two parallel gels (bold vertical line)]."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(A) Representative confocal images of APP-DKO cells expressing GFP-tagged C99WT or C99MUT (in green) and fluorescent markers of mitochondria (MitoDsRed, in red) and ER (Sec61β-BFP, in blue), and treated with DAPT to prevent C99 cleavage. Note the different distribution of C99WT or C99MUT forms. Scale bar = 10 µm. Insets show 5X amplifications of individual (C99 in green, and mitochondria in red) and merged images. Black and white bottom panels represent the areas where C99 and mitochondria signals colocalize. Upon thresholding each channel, a mask for mitochondria was generated and the C99 channel was superimposed on the mitochondria mask, so the positive pixels found in both channels are shown in black."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(C) Representative confocal images of APP-DKO cells expressing GFP-tagged C99WT or C99MUT and ER and mitochondria markers as in (A) (upper panel). Individual zoomed single channels (middle panel) as well as areas of ER-mitochondria colocalization (bottom panel) are shown for each condition. Scale bar = 10 µm."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(D) Quantification of ER-mitochondria colocalization per cell analyzed. Upon thresholding each channel, a mask for ER was generated and the mitochondria channel was superimposed on the ER mask, so the positive pixels found in both channels were quantified and referenced over the total area of the ER mask. The number of cells analyzed is indicated in white text within each column. Levels of ER-mitochondria colocalization in APP-WT cells are shown for reference as a dashed line. EV, empty vector. One-way ANOVA (n=3 independent experiments and 5-8 images/experiment; * p&lt; 0.05)."
NCBI gene IDs:
"APP: 11820"
Figure legend: "(B) Representative immunoblot to reveal C99 levels in total homogenate (TH) and MAM fractions of APP-DKO cells expressing C99WT or C99MUT before (input) and after cholesterol pull-down. Acsl4 was used as a MAM marker. Streptavidin-HRP was used to detect total biotinylation (biotin conjugated to PhotoClick cholesterol). (C) Quantification of pulled-down C99 levels versus input from the experiment in B. Two-way repeated measures ANOVA (Fraction, mutation) (n=5; * p&lt; 0.05, ** p&lt; 0.01). "
NCBI gene IDs:
"APP: 11820"
Figure legend: "qPCR mRNA levels for YAP target genes connective tissue grow factor (CTGF) and Ankyrin Repeat Domain 1 (ANRKD1), (three experimental replicates)"
NCBI gene IDs:
"Ankyrin Repeat Domain 1: 27063
ANRKD1: 27063
connective tissue grow factor: 1490
CTGF: 1490"
Figure legend: "mRNA expression of ANG in colonic tissues from normal controls (n = 25) and UC (n = 27) or CD (n = 46) patients."
NCBI gene IDs:
"ANG: 283"
Figure legend: "Ang mRNA expression in colonic tissue from 2.5% DSS-treated WT mice at indicated time point (n = 4 mice/time point). DSS: dextran sulfate sodium"
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Kaplan-Meier curve of 3.5% DSS-treated Ang-deficient mice (Ang-/-, n = 14) or littermate controls (WT, n = 13)."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Body weight loss of WT and Ang-/- mice with (n = 9) or without (n = 6) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "disease activity index (DAI) of WT and Ang-/- mice with (n = 9) or without (n = 6) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Colon length of colonic section from the WT and Ang-/- mice on day 9 with (n = 9) or without (n = 6) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "serum FITC-Dextran level of colonic section from the WT and Ang-/- mice on day 9 with (n = 9) or without (n = 6) 2.5% DSS treatment. FITC-Dextran: fluorescein isothiocyanate-labeled dextran."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "representative HE staining image, and (H) histopathological score of colonic section from the WT and Ang-/- mice on day 9 with (n = 9) or without (n = 6) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Quantitative mRNA expression of cytokine genes in colonic tissue from the WT and Ang-/- mice on day 0 (n = 3) or day 9 (n = 9) during 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Soluble cytokine level in supernatant of cultured colonic tissue isolated from WT and Ang-/- mice on day 0 (n = 3) or day 9 (n = 7) during 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Body weight loss of WT→WT, Ang-/-→WT and WT→Ang-/- chimera mice with (n = 7) or without (n = 5) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "DAI of WT→WT, Ang-/-→WT and WT→Ang-/- chimera mice with (n = 7) or without (n = 5) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Ang mRNA expression in mouse lymphoid and myeloid cells; data represent two independent experiments (n = 2)."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Body weight loss of Angfl/fl and LyzMcre;Angfl/fl mice with (n = 7) or without (n = 3) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727
cre: 2777477
Lyz: 17105"
Figure legend: "DAI of Angfl/fl and LyzMcre;Angfl/fl mice with (n = 7) or without (n = 3) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727
cre: 2777477
Lyz: 17105"
Figure legend: "Colon length of colonic section from the Angfl/fl and LyzMcre;Angfl/fl mice on day 8 with (n = 7) or without (n = 3) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727
cre: 2777477
Lyz: 17105"
Figure legend: "serum FITC-Dextran level, of colonic section from the Angfl/fl and LyzMcre;Angfl/fl mice on day 8 with (n = 7) or without (n = 3) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727
Lyz: 17105"
Figure legend: "representative HE staining image, and (M) histopathological score of colonic section from the Angfl/fl and LyzMcre;Angfl/fl mice on day 8 with (n = 7) or without (n = 3) 2.5% DSS treatment."
NCBI gene IDs:
"Ang: 11727
Lyz: 17105"
Figure legend: "Quantitative mRNA expression of ANG and PLXNB2 in isolated IECs or colonic laminar propria (cLP) cells from WT mice (n = 9)."
NCBI gene IDs:
"ANG: 11727
PLXNB2: 140570"
Figure legend: "Representative images showing cleaved-caspase 3 staining in colonic section from WT or Ang-/- colitis mice (n = 3) at indicated time point; arrows indicate cleaved-caspase 3 positive cells; two fields per colon section are quantified."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "the number of apoptotic cells in colonic section from WT or Ang-/- colitis mice (n = 3) at indicated time point"
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Cell apoptotic status of IECs from WT or Ang-/- colitis mice (n = 5) on day 4."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Representative images showing Ki67 staining in colonic section from WT or Ang-/- colitis mice (n = 3) at indicated time point; five representative crypts per colon section are quantified."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "the number of proliferating cells per crypt in colonic section from WT or Ang-/- colitis mice (n = 3) at indicated time point; five representative crypts per colon section are quantified."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Cell-cycle status of IECs from WT or Ang-/- colitis mice (n = 5) on day 4."
NCBI gene IDs:
"Ang: 11727"
Figure legend: "Representative images showing cleaved-caspase 3 staining in colonic section from Angfl/fl and LyzMCre;Angfl/fl, or AAV-shControl- and AAV-shPlxnb2- treated colitis mice on day 4 (n = 3); arrows indicate cleaved-caspase 3 positive cells; two fields per colon section are quantified."
NCBI gene IDs:
"Ang: 11727
Cre: 2777477
Lyz: 17105
Plxnb2: 140570"
Figure legend: "the number of apoptotic cells in colonic section from Angfl/fl and LyzMCre;Angfl/fl, or AAV-shControl- and AAV-shPlxnb2- treated colitis mice on day 4 (n = 3)"
NCBI gene IDs:
"Ang: 11727
Cre: 2777477
Lyz: 17105
Plxnb2: 140570"
Figure legend: "Ki67 staining and (L) the number of proliferating cells per crypt in representative colonic section from Angfl/fl and LyzMCre;Angfl/fl, or AAV-shControl- and AAV-shPlxnb2- treated colitis mice on day 4 (n = 3); five representative crypts per colon section are quantified."
NCBI gene IDs:
"Ang: 11727
Cre: 2777477
Lyz: 17105
Plxnb2: 140570"
Figure legend: "PLXNB2 expressions in HCT116 (including wild type HCT116 (HCT116WT) and ANG knockout HCT116 (HCT116KO)) and THP-1 as detected by flow cytometry analysis. The upper three dash lines represent the intensity of unstained negative control of these cells, while the solid lines represent the intensity of PLXNB2 antibody staining."
NCBI gene IDs:
"ANG: 283"