EMBO/SourceData huggingface dataset:
Figure legend: " D High levels of p62 bodies detected in the tumors of the MOAP-1 KO mice. Tumor sections from livers dissected from the 8.5-month-old male WT and MOAP-1 KO mice injected with a single dose of DEN (25 µg/g body weight) at 15-day-old were subjected to IHC analysis with anti-p62 antibody (in red). Nuclear were stained with DAPI (in blue). Insets were enlarged in the bottom right. Scale bar: 20 µm. E Quantification of the p62 bodies in the WT and MOAP-1 KO tumors. (Left panel) number of p62 bodies per cell. n = 7 tumors from the livers of WT and MOAP-1 KO mice. (Right panel) size of p62 bodies. n = 456 and 1211 bodies detected in the WT and MOAP-1 KO tumors, respectively, from three independent samples. Error bar represents S.E.M. *P&lt;0.05, ***P&lt;0.001, student's t-test. "
NCBI gene IDs:
"MOAP-1: 64113"
Figure legend: " F Increased levels of Nrf2 and its downstream target protein, HMOX-1, in the MOAP-1 deficient tumors. While p62 protein is upregulated in the tumors compared to the adjacent non-tumor tissues in both WT and MOAP-1 KO livers, no significant difference is observed in the p62 protein levels between the WT and MOAP-1 KO tumors. Lysates of the tumors and non-tumor parts of the livers from mice injected with DEN as described in (D) were prepared in RIPA lysis buffer containing 1% SDS and subjected to Western blotting. Actin as loading control. P, PBS injected control; N, non-tumor; T, tumor. "
NCBI gene IDs:
"MOAP-1: 64113"
Figure legend: " G Upregulation of mRNA levels of HMOX-1 and NQO1 in the MOAP-1 deficient tumors. Livers harvested from the mice injected with PBS or DEN as described in (D) were subjected to RT-PCR analysis. n = 3 WT and 3 MOAP-1 KO mice injected with the PBS control, and 8 WT and 9 MOAP-1 KO mice injected with DEN. Error bar represents S.E.M. **P&lt;0.01, student's t-test. "
NCBI gene IDs:
"HMOX-1: 15368
MOAP-1: 64113
NQO1: 18104"
Figure legend: " H MOAP-1 deficient mice bear higher tumor burden in the DEN-mediated liver cancer model. Representative images of liver tumors dissected from the 8.5-month-old male WT and MOAP-1 KO mice injected with a single dose of DEN (25 µg/g body weight) at 15-day-old. Scale bar: 5 mm. I Measurements of the number, maximum diameter of the tumor nodules and total liver weight from WT and MOAP-1 KO mice (n=13 and 14 respectively) injected with DEN as described in (H). Error bar represents S.E.M. **P&lt;0.01, ***P&lt;0.001, student's t-test. "
NCBI gene IDs:
"MOAP-1: 64113"
Figure legend: " MOAP-1 deficiency promotes tumor initiation. measurements of the number, diameter of the largest nodules and total liver weight of the 4.5-month-old WT and MOAP-1 KO mice (n=18 and 23 respectively) injected with a single dose of DEN (25 µg/g body weight) at 15-day-old. "
NCBI gene IDs:
"MOAP-1: 64113"
Figure legend: "Gene expression level of IL-1β and IL-6 of indicated mouse lung with PAO1 infection (5 × 106 CFU) for 6 h and 24 h. n≥3. Error bars, S.E.M. *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001; ****, P&lt;0.0001."
NCBI gene IDs:
"IL-1β: 16176
IL-6: 16193"
Figure legend: "Histogram of selected differentially expressed genes of LL-37+/+ mouse lung versus wild type FVB mouse lung prior to infection. Blue bars indicated genes up-regulated in wild type FVB mouse lungs, red bars indicated genes up-regulated in LL-37+/+ mouse lungs."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Detection of LL-37 expression in the engineered mDASCs by real-time quantitative PCR"
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Anti-Krt5 (red) and anti-P63 (green) immunostaining of WT- and LL-37-mDASC colonies. Scale bar, 70 μm."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Stem cell colony-forming efficiency of WT- and LL-37-mDASCs during 5 serial passages. n=6."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Bright field and direct fluorescence images of mouse lungs following transplantation of 1 × 106 GFP-labeled WT-mDASCs (WT-Lung) or LL-37-mDASCs (LL-37-Lung) on indicated days. Scale bar, 1000 μm"
NCBI gene IDs:
"GFP: 
LL-37: 820"
Figure legend: "Anti-Ki67 immunofluorescence of engrafted GFP-labeled WT- and LL-37-mDASCs in lung parenchyma 21 days after transplantation. Scale bar, 50 μm."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Distribution of engrafted GFP-labeled cells in lung parenchyma by immunofluorescence 7 days after transplantation. WT-Lung: WT-mDASCs engrafted; LL-37-Lung: LL-37-mDASCs engrafted. Scale bar, 200 μm. Arrows showed the representative cells with overlapping fluorescence of GFP and LL-37."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Intratracheal instillation of equal amount of PAO1(5 ×106 CFU per mouse) into WT-Lung (WT-mDASCs engrafted) and LL-37-Lung (LL-37-mDASCs engrafted) followed by bacterial CFU analysis in whole lung homogenates 6, 24 and 48 hours after infection. n=3. Error bars, S.E.M. Intratracheal instillation of equal amount of PAO1 into WT-Lung and LL-37-Lung followed by bacterial CFU analysis in BAL fluid 6, 24 and 48 hours after infection. n=3. Error bars, S.E.M."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Arterial blood gas analysis of mice with WT-Lung and LL-37-Lung following PAO1 infection. n=6. Error bars, S.E.M. *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001; ****, P&lt;0.0001."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "H&amp;E staining showing histology of injured lung infected by PAO1 with WT-mDASCs or LL-37-mDASCs transplantation. Scale bar, 100 μm. Histopathological examination according to the lung injury scoring system based on blinded expert judgement. n=5 mice per group. Error bars, S.E.M."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "CD68 immunochemistry (brown) in infected lung with WT-mDASCs or LL-37-mDASCs transplantation. Scale bar, 50 μm. Quantification of brown-stained (CD68+) area by Image J software. n=5. Error bars, S.E.M."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Gene expression level of indicated pro-inflammatory cytokines of lung infected by PAO1 with WT-mDASCs or LL-37-mDASCs transplantation. n=3. Error bars, S.E.M. *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001; ****, P&lt;0.0001."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "The mRNA expression levels of LL-37 was measured by qPCR for WT- and LL-37-hDASCs. n=5. Error bars, S.E.M."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "Ex vivo biomimetic culture of LL-37-hDASCs recellularized lungs with constant media perfusion."
NCBI gene IDs:
"LL-37: "
Figure legend: "The recellularized lung by LL-37-hDASCs displayed growth inhibitory effect on PAO1 and E.coli. Initial dose of bacteria was 2× 104 CFU. Culture duration, 24h. n=6. Error bars, S.E.M. *, P&lt;0.05; **, P&lt;0.01; ***, P&lt;0.001; ****, P&lt;0.0001."
NCBI gene IDs:
"LL-37: 820"
Figure legend: "(A) Levels of Piwil1  (Miwi), Piwil2  (Mili) and Piwil4  (Miwi2) transcripts in RNA seq. data from undifferentiated aNPCs  (Days of differentiation -DIF- 0) and differentiating neuroblasts  (DIF 4-14); insets in left and right panels show the same data with smaller scales in the ordinate axes. Data information: data are expressed as mean ± SEM, n = 3 biological replicates."
NCBI gene IDs:
"Miwi: 57749
Piwil1: 57749
Mili: 57746
Piwil2: 57746
Miwi2: 330890
Piwil4: 330890"
Figure legend: "(G) Scheme of the experiment (top) and Mili  mRNA expression  in sorted Td+ and Td- cells after in vivo transduction with split-Cre  viruses in the hippocampus  (bottom). Data information: data are expressed as mean ± SEM, n = 3 biological replicates. *p &lt; 0.05, **p &lt; 0.01, **** p &lt; 0.0001 as assessed by one-way ANOVA with Bonferroni test G)"
NCBI gene IDs:
"Cre: 2777477
Mili: 57746"
Figure legend: "(E) Mili  mRNA expression  (left bar graph); western blot (inset) and quantification (right bar graph) of Mili  protein abundance in aNPCs upon viral transduction of scrambled shRNA (Control) or shRNA targeting Mili  (Mili  KD). Data information: data are expressed as mean ± SEM unless differently indicated, n = 3 biological replicates ∗p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test."
NCBI gene IDs:
"Mili: 57746"
Figure legend: "(C) Mili  mRNA expression  (left bar graph); western blot (inset) and quantification (right bar graph) of Mili  protein abundance in lysates from the DG  of mouse hippocampi  48 hours after the injection of scrambled (Control) or GapmeR1 against Mili  (Mili  KD). Data information: data are expressed as mean ± SEM, n = 3 (C, biological replicates. ∗p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test."
NCBI gene IDs:
"Mili: 57746"
Figure legend: "(E) Representative immunofluorescence  micrograph of postnatal hippocampal  sections immunostained  for GFAP  at 30 dpi of scrambled (Control) and GapmeR1 against Mili  (Mili  KD); Right panels: Fold change in GFAP  fluorescence intensity level (upper graph) in a hippocampal  region of interest (ROI) of 500µm2 in brain  slices upon Mili  KD compared to Control; Gfap  mRNA levels (lower graph) in the DG  from mouse hippocampi  48 hours after the injection of scrambled (Control) or GapmeR1 (Mili  KD). Data information: data are expressed as mean ± SEM, n = 3 E mRNA and 5 (E biological replicates. ∗p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test. SGZ, subgranular zone. The scale bars represent 100 µm (E,"
NCBI gene IDs:
"Gfap: 14580
Mili: 57746"
Figure legend: "(F) (Left) representative immunofluorescence  micrograph of postnatal hippocampal  sections immunostained  for GFAP  (green), BrdU  (red), NeuN  (white) and nuclear DNA  (blue) at 30 dpi of scrambled (Control) or GapmeR1 against Mili  (Mili  KD); (right graphs) percentages of NeuN+BrdU+ (white arrowheads in the images), or GFAP+BrdU+ (yellow arrowheads in the images) double-positive cells over total BrdU+ cells. Data information: data are expressed as mean ± SEM, n = F) biological replicates. ∗p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test. GCL, granular cell layer, SGZ, subgranular zone. The scale bars represent 100 µm F)."
NCBI gene IDs:
"Mili: 57746"
Figure legend: "(B) Representative bright-field microscopy images (left) and quantification (right) of  ß-galactosidase+ aNPCs as percent of total cells upon Mili  KD, or control 48 h after induction of spontaneous differentiation. Data information: data are expressed as mean ± SEM, n = 5 (B biological replicates p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test. The scale bars represent 50 µm (B,"
NCBI gene IDs:
"Mili: 57746"
Figure legend: "(C) Representative  fluorescence microscopy images (left) and quantification (right) of control or Mili  KD neuroblasts  48h after spontaneous differentiation, immunostained  with anti-BrdU  (white) and Ki67  (purple) antibodies. (Right) Percentage of BrdU+ and Ki67- cells over BrdU+ cells. Data information: data are expressed as mean ± SEM, n = C) biological replicates p &lt; 0.05, ∗∗p &lt; 0.01, *** p &lt; 0.001, **** p &lt; 0.0001, as assessed by two-tailed Student's t-test. The scale bars represent 50 µm C)"
NCBI gene IDs:
"Mili: 57746"
Figure legend: "(C) Expression  levels of human GRα   and LRH-1 mRNA in human T-ALL  cell lines were determined by probe-based real-time quantitative PCR, calculated as relative expression  compared to  beta-actin and used to determine the GR:LRH-1 ratio (right). Individual and mean values of four biological replicates ± SD are shown and statistically significant differences between CEM-C7  and GC-resistant cell lines were determined by one-way ANOVA."
NCBI gene IDs:
"beta-actin: 60
GR: 2908
GRα: 2908
LRH-1: 2494"
Figure legend: "(F) GR  activity in human T-ALL  cells transfected with a control luciferase  reporter plasmid (pGL3) (F) GR  responsive (GRE) luciferase  reporter and treated with 3d2  and/or Dexa.  β-galactosidase  (bGal) was co-transfected as an internal transfection control. Luciferase reporter activity was normalized to bGal  activity and calculated as relative to the DMSO-treated pGL3 control. Individual and mean values analyzed by two-way ANOVA of three biological replicates ± SD are shown for Jurkat  and CEM-C7  whereas technical triplicates ± SD of a representative experiment (n=3 biological replicates) are shown for MOLT-4. Data information: For all displayed experiments dimethyl sulfoxide  (DMSO; was used as a solvent control. ns = not significant, * p &lt; 0.05, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"luciferase: 
bGal: 2720
β-galactosidase: 2720"
Figure legend: "(G) LRH-1  activity in human T-ALL cells transfected with a control luciferase  reporter plasmid (pGL3) and (G) LRH-1 responsive (5xRE) luciferase  reporter and treated with 3d2 β-galactosidase (bGal) was co-transfected as an internal transfection control.  Luciferase reporter activity was normalized to bGal  activity and calculated as relative to the DMSO-treated pGL3 control. Individual and mean values analyzed by two-way ANOVA of three biological replicates ± SD are shown for Jurkat  and CEM-C7 whereas technical triplicates ± SD of a representative experiment (n=3 biological replicates) are shown for  MOLT-4. Data information: For all displayed experiments dimethyl sulfoxide (DMSO; was used as a solvent control. ns = not significant, * p &lt; 0.05, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"luciferase: 
bGal: 2720
β-galactosidase: 2720"
Figure legend: "(B) Daily cell counting-based comparison of cellular proliferation   of  T-ALL  cells after (B) small hairpin RNA-mediated LRH-1  knockdown (shLRH-1). Individual and mean values of technical triplicates of one representative experiment (n=3 biological replicates) ± SD anlyzed by two-way ANOVA are shown. Data information: ns = not significant, * p &lt; 0.05, *** p &lt; 0.001."
NCBI gene IDs:
"LRH-1: 2494"
Figure legend: "(F)  Flow cytometric  analysis of 3d2-induced cell death by AnnexinV staining  in wild-type (WT), Bcl-2 overexpressing (Bcl-2) and Z-VAD-FMK (zVAD)-treated  CEM-C1. Mean values of technical triplicates of one representative experiment (n=3 biological replicates) ± SD analyzed by two-way ANOVA are shown. Data information: ns = not significant, * p &lt; 0.05, *** p &lt; 0.001."
NCBI gene IDs:
"Bcl-2: 596"
Figure legend: "(C) Flow cytometric  analysis of cell death  by AnnexinV staining  in control or Bim  knockout (Bim  KO) CEM-C7  cells treated with 3d2  and 30 µM Dexa. Mean values of technical triplicates of one representative of n=4 biological replicates ± SD are shown. Data information: Two-way ANOVA C, ns = not significant, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"Bim: 10018"
Figure legend: "(D) Immunoblots  of BimEL, BimL  and BimS, Bcl-2 and Tubulin  from MOLT-4 expressing a non- (shcontrol) or LRH-1- (shLRH-1) targeting small hairpin RNA construct."
NCBI gene IDs:
"LRH-1: 2494"
Figure legend: "(E) Flow cytometric  analysis of  cell death by AnnexinV staining  in control or glucocorticoid receptor knockout (GR  KO) MOLT-4  cells treated with 3d2  and 30 µM Dexa. Mean values of n=3 biological replicates ± SD are shown. Data information: Two-way ANOVA E); ns = not significant, ** p &lt; 0.01, *** p &lt; 0.001."
NCBI gene IDs:
"glucocorticoid receptor: 2908
GR: 2908"
Figure legend: "(F/G) mRNA expression  levels of human GR (F) and Bim (G) in shcontrol and shLRH-1 CEM-C1 cells were determined by  real-time quantitative PCR after treatment with 1 µM Dexa  for indicated times. Fold induction relative to the untreated shcontrol sample (0h) was calculated after normalization to beta-actin. Individual values of technical triplicates of one representative of two biological replicates ± SD are shown. Data information: For all displayed experiments dimethyl sulfoxide (DMSO; represented by 0 was used as a solvent control."
NCBI gene IDs:
"beta-actin: 60
Bim: 10018
GR: 2908
LRH-1: 2494"
Figure legend: "(C) Expression  levels of human GRα, LRH-1 and Bim  mRNA in human  PDX were determined by real-time quantitative PCR, calculated as relative expression  compared to  beta-actin and used to determine the GR:LRH-1 ratio (right). Data information: All displayed experiments display one biological replicate."
NCBI gene IDs:
"beta-actin: 60
Bim: 10018
GR: 2908
GRα: 2908
LRH-1: 2494"
Figure legend: "(a-c) We used quantitative real-time PCR to determine the expression patterns of ATG5 (a), ATG7 (b) and ATG16L1 (c) in various human immune and epithelial cell lines. ATG5 was broadly expressed in the cell lines studied (a), with between 1.5- and fourfold variations in expression level across the cell lines. ATG7 expression (b) was broadly comparable to that of ATG5, with the exception of THP1 cells, which showed 35-fold higher expression than SW480 cells. ATG16L1 expression (c) was also broadly similar to that of ATG5 and ATG7 (except for ATG7 expression in THP1 cells), with moderate variations across the cell lines tested (1.5- to 3.5-fold)."
NCBI gene IDs:
"ATG16L1: 55054
ATG5: 9474
ATG7: 10533"
Figure legend: "(d) Expression of ATG16L1 in human primary immune cells. In a resting human immune cell RNA panel (Clontech), ATG16L1 showed a clear peak of expression in the T cell compartment, with CD4+ and CD8+ cells expressing high levels of transcript (15- to 20-fold more than control samples). We detected modest levels of ATG16L1 in CD19+ cells and low levels in CD14+ monocytes and unfractionated mononuclear cells. In all cases, we performed real-time quantitative RT-PCR reactions in duplicate and plotted the means; error bars represent 1 s.d. We normalized expression levels by comparison with GAPDH controls and plotted arbitrary relative expression units, where the SW480 or placental RNA expression equals 1 (for cell lines or primary cells, respectively). We isolated all RNAs from resting cells in the absence of stimulation or activation."
NCBI gene IDs:
"ATG16L1: 55054
GAPDH: 2597"
Figure legend: "(a) We achieved specific siRNA knockdown of overexpressed, Flag-tagged ATG16L1 in HEK293 cells within 48 h of transfection with oligonucleotide duplexes. Flag-ATG16L1 was undetectable by protein blotting after treatment with specific siRNA constructs, but expression was maintained with control duplexes."
NCBI gene IDs:
"ATG16L1: 55054"
Figure legend: "(b) Endogenous ATG16L1 mRNA knockdown (mean relative abundance ± s.d.) by siRNA 2 in HeLa cells within 48 h of transfection, as assessed by real-time quantitative RT-PCR normalized to GAPDH. Compared with control duplex, siRNA 2 yielded an 89% reduction in transcripts. RT-PCR was performed in triplicate; results represent two independent experiments."
NCBI gene IDs:
"ATG16L1: 55054
GAPDH: 2597"
Figure legend: "c) Knockdown of ATG16L1 prevented effective autophagy of S. typhimurium in HeLa cells. Forty-eight hours after cotransfection with control siRNA or duplex 2 and LC3-GFP plasmid, HeLa cells were infected for 1 h with Salmonella typhimurium SL1344, fixed and examined microscopically. Mean percentages (± s.e.m.) of bacteria per cell encapsulated by LC3+ membranes (autophagosomes) are shown. Bacterial counts were pooled from two separate experiments; each counted a minimum of 100 infected cells. Significance was assessed by two-tailed Students t-test, assuming unequal variances."
NCBI gene IDs:
"ATG16L1: 55054
LC3: 440738///81631///84557"
Figure legend: "(d) Representative images of intracellular S. typhimurium show normal autophagic encapsulation by LC3+ membranes in control siRNA-treated cells but loss of autophagic targeting after ATG16L1 knockdown. Channels are separated to show both the LC3-GFP fusion protein (autophagosomal marker) and S. typhimurium SL1344 dsRed2 (SL1344), with a merged image in the final column. Rows 2 and 4 show magnified views of boxed areas in rows 1 and 3, respectively. Images were obtained using confocal microscopy and are a flat projection of z stacks taken throughout the plane of the infected cell. Scale bars = 5 μm."
NCBI gene IDs:
"ATG16L1: 55054
LC3: 440738///81631///84557"
Figure legend: "A. H3122 cells expressing endogenous EML4-ALK V1 and HEK293 transfected with YFP-EML4-ALK V1 WT and KD were stained for either anti-ALK or anti-GFP (green), anti-α-tubulin (red), and DAPI (blue). Scale bars, 10 μm; magnified views of a selected area are shown. B. Violin plots showing the number of EML4-ALK V1 cytoplasmic foci per cell. Data represents 30-50 counts from three biological replicates. ***P&lt;0.001, ****P&lt;0.0001 in comparison to HEK293 YFP-EML4-ALK-V1 KD by one-way ANOVA analysis. "
NCBI gene IDs:
"YFP: 
ALK: 238
EML4: 27436"
Figure legend: "C. H2228 cells expressing endogenous EML4-ALK V3 and HEK293 transfected with YFP-EML4-ALK V3 WT and KD were stained for either anti-ALK or anti-GFP (green), anti-α-tubulin (red), and DAPI (blue). Scale bars, 10 μm; magnified views of a selected area are shown. D. Violin plots showing the number of EML4-ALK V3 cytoplasmic foci per cell. Data represents 30-50 counts from four biological replicates. ***P&lt;0.001, ****P&lt;0.0001 in comparison to HEK293 YFP-EML4-ALK-V3 KD by one-way ANOVA analysis. "
NCBI gene IDs:
"YFP: 
ALK: 238
EML4: 27436"
Figure legend: "H. Whisker plot showing the calculated velocity of single events in the kymograph. Data represent 20 counts from 10 kymographs. n=2. Error bar represents SD of two biological replicates. *P&lt; 0.05 in comparison to YFP EML4-ALK V3 KD by unpaired t test."
NCBI gene IDs:
"YFP: 
ALK: 238
EML4: 27436"
Figure legend: "A, B, C, D. HEK293 cells were transfected with EML4-ALK V1 WT or V3 WT or Kinase Dead (KD) for 48 hours. Cells were either untreated (DMSO) or treated with ALK inhibitors for 4 hours before fixation and staining with anti-GFP (green), anti-α-tubulin (red), and DAPI (blue). Scale bars, 10 μm; magnified views of a selected area are shown."
NCBI gene IDs:
"ALK: 238
EML4: 27436"
Figure legend: "(B)  Western blot analysis of the generated isogenic cell lines described in A after inducing GFP-TDP-43 expression for 48 h showing the tightness of the doxycycline  (DOX)-modulated expression system. Note also the different protein levels of the expressed variants. endTDP-43: endogenous TDP-43."
NCBI gene IDs:
"GFP: 
TDP-43: 23435"
Figure legend: "(D) GFP-TDP-43 expression was induced with DOX  for 24 h before cycloheximide  (CHX) treatment for the indicated times  and western blot analysis. (E) Quantification of the GFP  signal from D. N=3 independent experiments. Two-way ANOVA with Tukey's multiple comparisons post hoc test."
NCBI gene IDs:
"GFP: 
TDP-43: 23435"
Figure legend: "(B) Representative maximum intensity Z-projections from confocal  fluorescence imaging of human neurons  transduced with TDP-43-HA  variants and immunolabeled  for the HA  tag and the neuron-specific marker MAP2. Scale bar: 10 µm. Cell nuclei  are stained  with DAPI"
NCBI gene IDs:
"HA: 
TDP-43: 23435"
Figure legend: "(D) GFP-TDP-43 expression was induced with doxycycline (DOX) for 4 h before nucleocytoplasmic  fractionation and subsequent analysis of GFP-TDP-43 and endogenous TDP-43 (endTDP-43) levels by  western blot. T: total lysate, C: cytoplasmic  fraction, N: nuclear  fraction. (E) Quantification of the GFP  signal from D. Repeated measures one-way ANOVA with Greenhouse-Geisser correction and Tukey's multiple comparisons post hoc test. Cyt: cytoplasm, Nuc: nucleus."
NCBI gene IDs:
"GFP: 
TDP-43: 23435"
Figure legend: "(C)  Fluorescence microscopy  images of 10 μM purified full-length TDP-43  and its oligomerization-deficient counterpart showing different abilities to undergo LLPS  and its disruption by 1,6-HD treatment for 10 min. Scale bar: 10 μm. (D) Quantification of the number of condensates in the conditions shown in C per 4000 µm2 field. N=10 independent experiments. Kruskal-Wallis test with Dunn's multiple comparisons post hoc test."
NCBI gene IDs:
"TDP-43: 23435"