EMBO/SourceData huggingface dataset:
Figure legend: "Increased interaction between Ddx17 and PCAF by Myoparr. PCAF protein was pulled down by 3×Flag-Ddx17 in the presence or absence of Myoparr using a Flag antibody and then detected by western blot."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "ChIP-qPCR detection of Ddx17 (H) and PCAF (I) occupancies at the myogenin locus in Myoparr-depleted differentiating C2C12 cells. The data were normalized to input values. n = 3, mean ± SD. *p &lt; 0.05 (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "Heatmap displaying expression changes of 754 genes significantly altered either in Myoparr- or Ddx17-depleted cells (log 2 ratio scale)."
NCBI gene IDs:
"Ddx17: 67040
Myoparr: 114004354"
Figure legend: "Genes significantly differentially expressed in both Myoparr- and Ddx17-depleted cells show correlated expression (R = 0.94, log 2 ratio scale)."
NCBI gene IDs:
"Ddx17: 67040
Myoparr: 114004354"
Figure legend: "Myoparr and Ddx17 are required for C2C12 cell cycle withdrawal. C2C12 cells transfected with each siRNA were cultured in growth medium for 24 h. After differentiation induction, cells were maintained in differentiation medium for 40 h and then treated with EdU for 6 h. EdU-positive cells are shown as percent of the control. Nuclei were counterstained with Hoechst 33342. n = 3, mean ± SD. **p &lt; 0.01 (unpaired two-tailed Student's t-test). Bar, 100 μm."
NCBI gene IDs:
"Ddx17: 67040
Myoparr: 114004354"
Figure legend: "qRT-PCR for pri-miR-133b, pri-miR-206, and H19 expression in differentiating C2C12 cells transfected with indicated siRNAs. n = 3, mean ± SD. *p &lt; 0.05. **p &lt; 0.01."
NCBI gene IDs:
"H19: 14955
pri-miR-133b: 723817
pri-miR-206: 387202"
Figure legend: "ChIP-qPCR detection of Pol II occupancy at the indicated promoters in Myoparr-depleted C2C12 cells. The data were normalized to input values. n = 3, mean ± SD. *p &lt; 0.05. Myoparr KD decreased Pol II occupancy at the miR-133b promoter with a marginal trend toward significance (p = 0.052)."
NCBI gene IDs:
"miR-133b: 723817
Myoparr: 114004354"
Figure legend: "ChIP-qPCR detection of Ddx17 (C) occupancies at the indicated promoters in Myoparr-depleted C2C12 cells. n = 3, mean ± SD. *p &lt; 0.05. **p &lt; 0.01."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "ChIP-qPCR detection of PCAF (D) occupancies at the indicated promoters in Myoparr-depleted C2C12 cells. n = 3, mean ± SD. *p &lt; 0.05. **p &lt; 0.01."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "qRT-PCR showing decreased expression of miR-133b and miR-206 in both Myoparr- and Ddx17-depleted C2C12 cells. n = 3, mean ± SD. *p &lt; 0.05. **p &lt; 0.01."
NCBI gene IDs:
"Ddx17: 67040
miR-133b: 723817
miR-206: 387202
Myoparr: 114004354"
Figure legend: "Western blots showing increased ERK1/2 activity (pERK1/2) and Cdc6 expression in differentiating C2C12 cells 48 h after Myoparr and Ddx17 KD. Tubulin expression served as an internal control."
NCBI gene IDs:
"Ddx17: 67040
Myoparr: 114004354"
Figure legend: "Increased Pola1 expression detected by qRT-PCR. n = 3, mean ± SD. **p &lt; 0.01."
NCBI gene IDs:
"Pola1: 18968"
Figure legend: "Expression of myogenin in innervated and denervated TA muscles detected by qRT-PCR 7 d after denervation. n = 4, mean ± SD. ***p &lt; 0.001 (unpaired two-tailed Welch's t-test). qRT-PCR showing increased Myoparr expression in denervated TA muscles 7 d after denervation. n = 3, mean ± SD. ***p &lt; 0.001 (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"myogenin: 17928
Myoparr: 114004354"
Figure legend: "Immunoblot showing decreased expression of myogenin by Myoparr depletion in denervated TA muscle 7 d after denervation. Expression of tubulin served as an internal control."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Weights of innervated and denervated TA muscles electroporated either with control or Myoparr shRNA. Muscle weights were measured 7 d after denervation. n = 5 for each group, mean ± SEM. ***p &lt; 0.001. n.s., not significant (unpaired two-tailed Student's t-test)."
NCBI gene IDs:
"Myoparr: 114004354"
Figure legend: "Representative immunostaining images for laminin (red) and EmGFP (green) of innervated or denervated TA muscles electroporated with control or Myoparr shRNA, both containing EmGFP. Bar, 100 μm."
NCBI gene IDs:
"EmGFP: 
Myoparr: 114004354"
Figure legend: "B-C. (B) Micronuclei frequency formation by live cell imaging in untreated (NT) and Auxin-treated (IAA) condition. (C) Bar graph represents the type of chromosome mis-segregation (independently of the dCas9 signal) observed in the indicated conditions, with (IAA) or without (NT) auxin, in the cell lines expressing dCas9 mScarlet-I and sgRNA targeting chromosome 1 or 3. Error bars represent the SEM of four independent experiments in which cells labeled for chromosome 1 and 3 were analyzed together (n = 45 - 149 cells)."
NCBI gene IDs:
"mScarlet-I: 
Cas9: 901176"
Figure legend: "D. Representative live cell imaging of RPE-1 cells dCas9 mScarlet-I with sgRNA targeting chromosome 1 (red dots) starting from metaphase and showing example of correct (untreated) or mis-aligned chromosome (auxin) leading to the formation of a micronucleus. Yellow arrows mark mis-aligned- and micronucleus-containing chromosome 1. Cells were imaged every five minutes. The numbers indicate the time point at which the presented images were taken. Cells were fixed at the end of the movie to detect Cas9 with an antibody. Scale bar represents 10 µm."
NCBI gene IDs:
"mScarlet-I: 
Cas9: 901176"
Figure legend: "E. Bar graph representing the proportion of chromosome mis-segregation observed in both cell lines after auxin addition. Only movies in which the dCas9 mScarlet-I dots were clearly visible during the whole division were taken into consideration for the analysis (N = 6 - 10 cells)."
NCBI gene IDs:
"mScarlet-I: 
Cas9: 901176"
Figure legend: "B. Representative image of centromere 11 (red) and centromere X (green) FISH on RPE-1 CENP-AAID CENP-B KO mutant in control (top) or IAA treated condition (bottom). Scale bar represents 5 µm. Bar graphs show automatic FISH quantification of chromosome mis-segregation in the indicated cell lines with and without IAA addition for 24 h. Error bars represent the SEM of three independent experiments (n&gt; 200 cells per experiment). Dunnett's multiple comparisons test was used to compare conditions. Grey lines separate independent experiments. Fold changes between grey and red bars are also indicated."
NCBI gene IDs:
"CENP-A: 1058
CENP-B: 1059"
Figure legend: "K. (Left) Representative image of CENP-B box (red) staining after siRNA against CENP-A/HJURP for 48 h. CENP-B box signals at individual micronuclei are magnified in insets. Scale bar represent 5 µm. (Right) Bar graph represents the mean of the CENP-B box FISH intensity contained in the main nucleus versus the one present in the micronucleus ± SEM. Color dots represent independent experiments. (n &gt; 43 micronuclei and 840 main nucleus) Mann-Whitney U test: ***p = 0.0001."
NCBI gene IDs:
"CENP-A: 12615
HJURP: 381280"
Figure legend: "(c) Western blot showing typical efficiency of FLCN knockdown in HeLa cells using a pool consisting of 4 oligonucleotides and 2 independent oligonucleotides from the pool. Graph shows relative FLCN expression (n=3, error bars show S.E.M.)"
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(d) Representative confocal fluorescence microscopy images showing LAMP1 (magenta) distribution in HeLa cells under normal growth or starvation conditions in cells depleted of FLCN or control cells transfected with a non-targeting siRNA. The location of the Golgi (green) is highlighted by giantin staining.(e) Schematic illustrating application of the cumulative intensity distribution method for quantification of lysosome distribution.(f) Graph showing cumulative distribution of LAMP1 intensity in normal growth and starvation conditions in HeLa cells. P-value is determined by the extra sum of F-squares test following non-linear regression and curve fitting. Error bars show S.E.M. from 30 cells in 3 replicates.(g) Graph showing cumulative distribution of LAMP1 intensity in starvation conditions for control cells transfected with non-targeting siRNA or cells depleted of FLCN. Error bars show S.E.M. from 30 cells in 3 replicates."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(a) Graphs showing cumulative distribution of LAMP1 intensity in control GFP (black), GFP-Rab34 (green) or GFP-Rab7 (blue) transfected cells. Note black line and symbols partially obscure blue."
NCBI gene IDs:
"Rab34: 19376
Rab7: 7879///338382"
Figure legend: "(c) Representative confocal fluorescence microscopy images of FLCN depleted or control cells, transfected with GFP-Rab34. Scale bar = 10μm."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(d) (left) Graphs showing mean GFP-Rab34 expression (grey values) and cumulative distribution of LAMP1 intensity (right) in FLCN depleted or control siRNA cells transfected HeLa cells expressing GFP-Rab34. Error bars show S.E.M.. from 30 cells in 3 replicates. Scale bar = 10μm."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(e) Western blot and graph showing typical efficiency of Rab34 siRNA knockdown after 48 hours in HeLa cells. Error bars show S.E.M from 3 experiments, *** = p&lt;0.001."
NCBI gene IDs:
"Rab34: 19376"
Figure legend: "(f) Representative confocal fluorescence microscopy images showing LAMP1 (magenta) distribution in HeLa cells under normal growth or starvation conditions in cells depleted of Rab34 or control cells transfected with a non-targeting siRNA. Giantin is shown in green. Scale bar = 10μm.(g) Graphs showing cumulative distribution of LAMP1 intensity in normal growth or starvation conditions for control cells or cells depleted of Rab34 using siRNA.(h) Graph showing percentage of LAMP1 in cis-medial Golgi-region (giantin overlap) in the indicated conditions. Error bars show S.E.M from 30 cells in 3 replicates, *** = p&lt;0.001."
NCBI gene IDs:
"Rab34: 19376"
Figure legend: "(d) Analysis of Lysotracker-Red dynamics using PCC decay in HeLa cells transfected with either GFP or GFP-Rab34. Data is from 10 cells per condition. Error bars show S.E.M."
NCBI gene IDs:
"Rab34: 19376"
Figure legend: "(d) Western blot analysis of GST-RILP pull down from UOK257 cells showing DENN domain dependent association of FLCN with RILP N-terminal, Rab binding domain (RBD) and C-terminal fragments."
NCBI gene IDs:
"FLCN: 201163
RILP: 83547"
Figure legend: "(f) Western blot analysis of GST-RILP pull down showing direct interaction between RILP and GTP hydrolysis deficient (Q111L) Rab34 (at 200nM, in the presence of 1mM GTPγS) and FLCN-DENN domain (1μM), but not low nucleotide affinity (T66N) Rab34. FLCN-DENN enhances association of RILP with Q111L Rab34. Graph shows quantification of relative RILP / Rab34 Q111L binding. Error bars show SEM. ** = p&lt;0.01."
NCBI gene IDs:
"FLCN: 201163
Rab34: 19376"
Figure legend: "(d) Western blot showing FLCN and FLCNΔDENN expression in stable UOK257 derived cell lines."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(e) Representative widefield fluorescence microscopy images showing intracellular distribution of LAMP1 in UOK257, UOK257-FLCN and UOK257-FLCNΔDENN cells. Scale bar = 10μm. Graphs showing cumulative distribution of LAMP1 intensity in UOK257, UOK257-FLCN and UOK257-FLCNΔDENN cells. Error bars show S.E.M from 30 cells in 3 replicates."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "(f) (left) Western blot showing endogenous Rab7 and Rab34 expression in UOK257, UOK257-FLCN and UOK257-FLCNΔDENN cells and relative levels of activity as measured by GST-RILP pull down. HSC70 is used as a loading control. (right) Graphs show quantification of bound Rab7/Rab34 from 3 independent experiments (p&lt;0.05, *** = p&lt;0.001)."
NCBI gene IDs:
"FLCN: 201163"
Figure legend: "A. Representative pseudocolor images of PC6 cells transfected with EYFP-Parkin (green) and mitochondrially targeted CFP (red) with and without PINK1 and Miro1 shRNA and Miro2 shRNA. Zoomed images show translocation of EYFP-Parkin to mitochondria in PINK1-overexpressing PC6 cells"
NCBI gene IDs:
"PINK1: 298575
Parkin: 5071
Miro1: 303351
Miro2: 287156"
Figure legend: "B. Quantification of EYFP-Parkin translocation to mitochondria. The percentage of PC6 cells with EYFP-Parkin translocated to mitochondria in response to PINK1 overexpression is lower in the Miro shRNAs-expressing groups when compared with the scrambled shRNA-expressing group (scr shRNA). ****P &lt; 0.0001, n = 6 dishes per group, 20 fields per dish, one-way ANOVA"
NCBI gene IDs:
"PINK1: 298575
Miro: 303351"
Figure legend: "C. The kinetics of EYFP-Parkin translocation is different in PC6 cells transfected with scrambled or Miro shRNAs. Cells were plated in separate compartments of the same dish, enabling us to visualize Parkin translocation simultaneously over 3 h under similar conditions in response to antimycin with oligomycin treatment (A&amp;O, both 10 µM). The spatial heterogeneity of EYFP-Parkin (coefficient of variation of the intensity of individual pixels) was estimated for individual cells for each time point (n = 39-47 cells; P &lt; 0.0001 difference between the curves P &lt; 0.0001, two-way ANOVA; lines show mean, dashed area shows SEM)"
NCBI gene IDs:
"Miro: 303351"
Figure legend: "D. Miro shRNAs mitigate the effect of A&amp;O on EYFP-Parkin translocation. PC6 cells transfected with EYFP-Parkin with and without Miro shRNAs were treated with DMSO or A&amp;O (both 10 µM) for 3h. The figure demonstrates that the percentage of cells with Parkin translocated to mitochondria was lower in the Miro shRNAs-expressing group when compared with the scrambled shRNA-expressing group. ****P &lt; 0.0001, n = 4 dishes, 20 fields per dish, one-way ANOVA"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "E. Miro shRNAs inhibit PINK1-induced mitophagy in PC6 cells. The figure shows the changes in the excitation spectra of mitochondrially targeted pH-sensitive Keima (ratio of 561 nm/458nm fluorescence intensities). ****P &lt; 0.0001, n = 80 cells from 4 dishes, Kruskal-Wallis test"
NCBI gene IDs:
"Miro: 303351"
Figure legend: "F. Miro shRNAs inhibit basal mitophagy in primary cortical neurons expressing mitochondrially targeted Keima. Mitochondria in lysosomes (acidic pH) were counted manually, by a blinded observer (**P &lt; 0.01, n = 9 dishes, 9 fields per dish, from 2 independent experiments, one-way ANOVA)"
NCBI gene IDs:
"Miro: 303351"
Figure legend: "G. Miro shRNAs decrease the number of mitochondria co-localised with the autophagosome marker EGFP-LC3B in primary cortical neurons (**P &lt; 0.01, n = 5 dishes, 9 fields per dish, one-way ANOVA)."
NCBI gene IDs:
"Miro: 303351"
Figure legend: "A. The pattern of Parkin translocation caused by Miro1 overexpression is different from A&amp;O-induced translocation. The figure illustrates puncta-like EYFP-Parkin on mitochondria in antimycin and oligomycin-treated (both 10 µM for 3h) PC6 cells (middle panels), whereas in Miro1-overexpressing cells, EYFP-Parkin appears along rod-shaped mitochondria visualised with CFP-mito (lower panels). The merged panels present EYFP-Parkin (green) and CFP-mito (red)"
NCBI gene IDs:
"Miro1: 303351"
Figure legend: "B. Overexpression of myc-Miro1 and untagged Miro2, but not myc-Miro2, induce EYFP-Parkin translocation to mitochondria. ****P &lt; 0.0001 vs control group, n = 5-6 dishes, 20 fields per dish, one-way ANOVA or t-test"
NCBI gene IDs:
"Miro1: 55288
Miro2: 89941"
Figure legend: "C. Quantification of EYFP-Parkin signal heterogeneity in PC6 cells demonstrates that Miro1 overexpression induces Parkin translocation from the cytosol to mitochondria, which is significantly weaker than PINK1 overexpression- or A&amp;O (3 h)-induced translocation. **P &lt; 0.01 and ****P &lt; 0.0001 vs control group, n = 18-32 cells from 3 dishes per group, Kruskal-Wallis test"
NCBI gene IDs:
"PINK1: 298575
Miro1: 303351"
Figure legend: "Miro1 overexpression does not cause mitochondrial depolarization (D) Representative image of Miro1 and EYFP-Parkin-transfected cells stained with TMRE. The merged panels present EYFP-Parkin (green) and TMRE (red)"
NCBI gene IDs:
"Parkin: 5071
Miro1: 303351"
Figure legend: "Miro1 overexpression does not cause mitochondrial depolarization (E) Relative TMRE intensity quantified in neurons transfected with Miro1 (detected by CFP-mito co-transfection); the control group is non-transfected cells in the same image. The relative TMRE signal was slightly increased in Miro1 overexpressing neurons but unaffected in Miro shRNAs-expressing neurons. Note that FCCP treatment (3 μM for 15 min) almost completely abolished TMRE fluorescence. ****P &lt; 0.0001 vs control group, n = 94-186 cells from 4 dishes per group, Kruskal-Wallis test"
NCBI gene IDs:
"Miro: 303351
Miro1: 303351"
Figure legend: "F. Co-expression of HA-Parkin with Miro1 does not suppress mitochondrial motility in axons of cortical neurons. Overexpression of Miro1 alone increased the motion time in both directions as well as the relative mitochondrial velocity. Co-transfection of Parkin did not affect significantly the motility parameters. Data are presented as Tukey boxplot. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ****P &lt; 0.0001, n = 689-846 individual mitochondria from 39-44 axons per group pooled from at least from 10 individual dishes from 4 independent experiments, Kruskal-Wallis test"
NCBI gene IDs:
"Parkin: 5071
Miro1: 303351"
Figure legend: "G. Parkin overexpression does not induce degradation of myc-Miro1 in control conditions. Representative Western blot image and analysis of myc-Miro1 expression in HEK cells. Treatment with A&amp;O (both 15 µM) for 3h in the absence of MG132 led to slight degradation of myc-Miro1. Co-expression of EYFP-Parkin did not induce degradation of Miro1 although an additional band above the Miro1 band was present. However, Parkin co-expression enhanced Miro1 degradation when cells were treated with A&amp;O. *P &lt; 0.05 and ****P &lt; 0.0001, ns: not significant, n = 4 independent experiments, one way ANOVA. Note that only the main band of myc-Miro1 at 80 kDa was analysed and that YFP was used to compensate in groups not expressing EYFP-Parkin"
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "A. Miro1 induces EYFP-Parkin translocation in the presence of PINK1 shRNA. PC6 cells were transfected with EYFP-Parkin and Miro1 or treated for 3h with A&amp;O (both 10 µM) in the presence of scrambled shRNA or PINK1 shRNA. Miro1 induced Parkin translocation also in the presence of PINK1 shRNA while A&amp;O treatment did not. ****P &lt; 0.0001 compared with the respective control group, n = 5-6 dishes, 20 fields per dish, one-way ANOVA"
NCBI gene IDs:
"PINK1: 298575
Parkin: 5071
Miro1: 303351"
Figure legend: "B. Miro1 induces EYFP-Parkin translocation in PINK1 -/- MEFs. MEFs obtained from PINK1 +/+ and -/- mice were transfected with EYFP-Parkin and Miro1 or treated with A&amp;O (both 10 µM for 3h). Miro1 induced Parkin translocation in both MEF lines but A&amp;O only in PINK1 +/+ MEFs. ****P &lt; 0.0001 compared with respective control group, n = 6 dishes, one-way ANOVA"
NCBI gene IDs:
"PINK1: 68943
Parkin: 5071
Miro1: 59040"
Figure legend: "C. Mutated EYFP-Parkin is recruited to mitochondria in response to Miro1 overexpression. PC6 cells were transfected with wild type, T240R or C431N EYFP-Parkin and Miro1 or treated for 3 hours with A&amp;O (both 10 µM). Miro1 induced translocation of all EYFP-Parkin mutants whereas A&amp;O treatment was not able to induce equivalent translocation of Parkin mutants. **P &lt; 0.01 and ****P &lt; 0.0001 compared with respective control group, n = 3-6 dishes, 20 fields per dish, one-way ANOVA"
NCBI gene IDs:
"Parkin: 5071
Miro1: 303351"
Figure legend: "D. Parkin mutants T240R and C431N interact with Miro1 both in DMSO- or A&amp;O-treated (both 15 µM for 3h) HEK cells. Note that the higher molecular weight bands of Miro1 were observed only in WT Parkin-expressing groups."
NCBI gene IDs:
"Parkin: 5071"
Figure legend: "A. Miro1 K572R mutant interacts with HA-Parkin similarly to Miro1 WT. HEK cells overexpressing myc-Miro1 WT or K572R mutant were treated with DMSO (control) or A&amp;O (both 15 µM) for 3 hrs. Myc-Miro1 was immunoprecipitated and probed for myc-Miro1 and HA-Parkin. Note the diminished degradation of Miro1 K572R in input and the additional band above Miro1 WT in the IP, which is missing in the case of the K572R mutant"
NCBI gene IDs:
"Miro1: 55288"
Figure legend: "B. Miro1 K572R ubiquitination by Parkin is reduced. HEK cells overexpressing HA-ubiquitin, myc-Miro1 or myc-Miro1 K572R and EYFP-Parkin were treated with DMSO (control) or A&amp;O (both 15 µM) for 3 hrs. HA-ubiquitin was immunoprecipitated and probes were immunoblotted for ubiquitin-HA and myc-Miro1"
NCBI gene IDs:
"Parkin: 5071
Miro1: 55288"
Figure legend: "C. Representative images of PC6 cells overexpressing Miro1 K572R or Miro1 WT together with EYFP-Parkin. K572R mutant induces Parkin translocation similar to WT in basal conditions but not after treatment with A&amp;O (both 7.5 µM) for 3 hours. Note the spatial heterogeneity values shown in the images"
NCBI gene IDs:
"Parkin: 5071
Miro1: 303351"
Figure legend: "D. Quantification of EYFP-Parkin signal heterogeneity in PC6 cells demonstrates that WT Miro1 overexpression amplifies Parkin translocation in response A&amp;O (both 7.5 µM) treatment for 3 hours, but K572R mutation of Miro1 abolishes this effect. ***P &lt; 0.001 and ****P &lt; 0.0001, n = 152-172 cells from 3 dishes per group, Kruskal-Wallis test. Data are presented as means ± SEM."
NCBI gene IDs:
"Miro1: 303351"
Figure legend: "A. A Ca2+-insensitive mutant of Miro1 (Miro1-EF) interacts with Parkin. HEK cells overexpressing myc-Miro1 or myc-Miro1-EF and EYFP-Parkin were treated with DMSO (control) or A&amp;O (both 15 µM) for 3 hrs. Myc-Miro1 was immunoprecipitated and probes were immunoblotted for Miro1 (red) and Parkin (green). The high-contrast grayscale image from the green channel of this blot shows that endogenous Parkin co-immunoprecipitated with Miro1 in all conditions. The bar chart shows the quantification of co-immunoprecipitated Parkin EYFP from 4 different blots normalised to total EYFP-Parkin signal (*P &lt; 0.05 and **P &lt; 0.01, repeated measures ANOVA"
NCBI gene IDs:
"Parkin: 5071
Miro1: 55288"
Figure legend: "B. Miro1-EF is less susceptible to ubiquitination by Parkin. HEK cells overexpressing HA-ubiquitin, myc-Miro1 or myc-Miro1-EF and EYFP-Parkin were treated with DMSO (control) or A&amp;O (both 15 µM) for 3 hrs. HA-ubiquitin was immunoprecipitated and probes were immunoblotted for ubiquitin (red), Miro1 (green) and Parkin (green; representative images are from the same experimental material on separate SDS-PAGE-WB membranes). The high-contrast grayscale shows the green channel of this blot, and the bar chart shows quantification of co-immunoprecipitated Miro from 3 different blots (**P &lt; 0.01 and ***P &lt; 0.001, repeated measures ANOVA). Note that EYFP-Parkin co-immunoprecipitated with ubiquitin only after A&amp;O treatment"
NCBI gene IDs:
"Parkin: 5071
Miro1: 55288"
Figure legend: "C. Mutation of Miro1 EF-hands diminishes Miro1 ubiquitination with S65-phosphorylated ubiquitin. HEK cells were transfected with EYFP-Parkin with either myc-Miro1-WT or myc-Miro1-EF with or without PINK1-V5. Cells were treated with either DMSO or A&amp;O (both 10 µM) for 5 hrs with the exception of the PINK1-V5 group. Myc-Miro1 was immunoprecipitated and probes were immunoblotted for Miro1 (red), S65-Phosphoubiquitin and PINK1-V5 (green; the weak bands marked with the asterisk are nonspecific)."
NCBI gene IDs:
"Parkin: 5071
Miro1: 55288"
Figure legend: "A. Kinetics of EYFP-Parkin translocation in response to A&amp;O is different in control and Miro1-EF-expressing PC6 cells. The inset shows how different compartments of the same dish were transfected with EYFP-Parkin and control plasmid or Miro1-EF and followed over 3 hrs using a confocal microscope after addition of A&amp;O (both 10 µM). The graph shows the average spatial heterogeneity of EYFP-Parkin fluorescence, reflecting the ratio of mitochondrial-to-cytosolic Parkin over time (n = 40-43 cells, difference between the curves P &lt; 0.0001, two-way ANOVA; lines show mean, dashed area shows SEM)"
NCBI gene IDs:
"Parkin: 5071
Miro1: 55288"