EMBO/SourceData huggingface dataset:
Figure legend: "(A) Old and new H4   distribution in prophase  and prometaphase ISCs  (esg-Gal4&gt;UASp-FRT-H4-eGFP-FRT-H4-mCherry). Separable domains of H4-eGFP  (old) and H4-mCherry  (new) were observed (B) Old and new H2A   distribution in prophase  and prometaphase ISCs  (esg-Gal4&gt;UASp-FRT-H2A-eGFP-FRT-H2A-mCherry), showing largely overlapping signals between H2A-eGFP  (old) and H2A-mCherry  (new). Data information: n for individual ISCs. Scale bar in (A), (B) 5 µm."
NCBI gene IDs:
"eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
H2A: 3772345
H4: 318846"
Figure legend: "(A) Old and new H3   distribution in a post-mitotic pair of cells with asymmetric Dl-nLacZ   labeling, showing that H3-eGFP  (old) is asymmetrically   inherited by the Delta-high cell, while H3-mCherry  (new) is enriched in the Delta-low cell (esg-Gal4&gt;UASp-FRT-H3-eGFP-FRT-H3-mCherry). (B) Old and new H3   distribution in a post-mitotic pair of cells with symmetric Dl-nLacZ  labeling, showing that both H3-eGFP  (old) and H3-mCherry  (new) are more symmetrically  distributed between the two cells. Scale bar in (A), (B), 5 µm; asterisk, ISC  side."
NCBI gene IDs:
"Dl-nLacZ: 
eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
H3: 3771723"
Figure legend: "(E) Old and new H4   distribution in a post-mitotic pair of cells with asymmetric Dl-nLacZ   labeling, showing that H4-eGFP  (old) is asymmetrically  inherited by the Delta-high cell, while H4-mCherry  (new) is enriched toward the Delta-low cell (esg-Gal4&gt;UASp-FRT-H4-eGFP-FRT-H4-mCherry). (F) Old and new H4   distribution in a post-mitotic pair with symmetric Dl-nLacZ  labeling, showing that H4-eGFP  (old) and H4-mCherry  (new) are symmetrically  distributed between the two cellss. Scale bar in (E), (F), 5 µm; asterisk, ISC  side."
NCBI gene IDs:
"Dl-nLacZ: 
eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
H4: 318846"
Figure legend: "(I) Old and new H2A   distribution in a post-mitotic pair of cells with asymmetric Dl-nLacZ   labeling, showing that H2A-eGFP  (old) and H2A-mCherry (new) are symmetrically  inherited by the two cells (esg-Gal4&gt;UASp-FRT-H2A-eGFP-FRT-H2A-mCherry). (J) Old and new H2A   distribution in a post-mitotic pair of cells with symmetric Dl-nLacZ  labeling, showing that H2A-eGFP  (old) and H2A-mCherry (new) are symmetrically  distributed between the two cells. Scale bar in (I), (J), 5 µm; asterisk, ISC  side."
NCBI gene IDs:
"Dl-nLacZ: 
eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
H2A: 3772345"
Figure legend: "(M) The ISC-specific combination of drivers esg-Gal4, Su(H)-Gal80  drive H3   transgene [esg-Gal4, Su(H)-Gal80&gt;UASp-FRT-H3-eGFP-FRT-H3-mCherry] with  heat shock treatment and recovery (see Materials and Methods) in a Delta-asymmetric  pair of cells. Old and new H3   distribution in a post-mitotic pair of cells with asymmetric Dl-nLacZ   labeling, showing that H3-eGFP  (old) is asymmetrically  inherited by the Delta-high cell, while H3-mCherry  (new) is enriched in the Delta-low cell. (N) ISC  specific drivers express the H3   transgene in a Delta-symmetric  pair of cells. Old and new H3   distribution in a post-mitotic pair of cells with symmetric Dl-nLacZ  labeling, showing that both H3-eGFP  (old) and H3-mCherry  (new) are more symmetrically  distributed between the two cells. Scale bar in (M), and (N): 5 µm; asterisk, ISC  side."
NCBI gene IDs:
"Dl-nLacZ: 
eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
Gal80: 854954
H3: 3771723
Su(H): 34881"
Figure legend: "(A) Old and new H3T3A  distribution in prophase ISC, showing separable domains between H3T3A-GFP   (old) and H3T3A-mKO  (new) (esg-Gal4&gt;UASp-FRT-H3T3A-eGFP-FRT-H3T3A-mCherry). Data information: Scale bar in (A), (C), (E), (F): 5 µm,"
NCBI gene IDs:
"eGFP: 
mCherry: 
esg: 34903
Gal4: 855828
H3: 3771723"
Figure legend: "(E) In a post-mitotic pair of cells with comparable  Dl-nLacZ, both H3T3A-GFP  (old) and H3T3A-mKO  (new) are symmetrically  distributed between the two cells. (F) In a post-mitotic pair of cells with asymmetric Dl-nLacZ expression, H3T3A-GFP  (old) is enriched in the Delta-high cell, while H3T3A-mKO  (new) is more toward the Delta-low cell. Data information: Scale bar in (E), (F): 5 µm, asterisk, ISC  side."
NCBI gene IDs:
"Dl-nLacZ: "
Figure legend: "(H) Distribution of the  Dl-nLacZ-positive (magenta) cells in an H3-expressing midgut, which are well spaced and interspersed within the  intestinal epithelium. (I) Distribution of the Dl-nLacZ-positive (magenta) cells in an H3T3A-expressing midgut, which are unevenly distributed in clusters with two or more Delta-positive cells. DAPI: white in (H-I). Data information: Scale bar in (H) and (I): 50 µm"
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(K) Percentages of clusters with 1-, 2-, or &gt;3-Delta-positive cell clusters for H3-expressing midguts   (avg. percentage of 1-Dl+ cells = 87.7% + 1.1%, avg. percentage of 2-Dl+ cells = 11.9% + 0.9%, avg. percentage of &gt;3-Dl+ cells = 0.4% + 0.2%, N = 4 midguts, n = 1,482 clusters) and H3T3A-expressing midguts  (avg. percentage of 1-Dl+ cells = 68.2% + 1.2%, avg. percentage of 2-Dl+ cells = 28.5% + 0.9%, avg. percentage of &gt;3-Dl+ cells = 3.3% + 0.4%, N = 5 midguts, n = 1,862 clusters). Bars represent mean proportions of cell clusters with 1, 2, or 3+ Dl+ cells out of all cell clusters counted. Error bars represent SEM. Comparing H3-expressing midguts  with H3T3A-expressing midguts, there was a statistically significant difference in the distributions of Delta-positive cells per cluster, determined by a Chi-Square test (X2(2, n = 1862) = 908.75, **** p &lt; 0.0001). As shown, there are significant increases in 2 and &gt;3-Delta-positive cells per cluster in the H3T3A-expressing intestines  when compared to the H3-expressing intestines."
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(L) Percentages of single Delta-positive cells out of total Delta-positive cells for H3-expressing midguts  (avg. single Dl+ cells = 77.9% + 1.9%, N = 4 midguts, n = 1,674 Dl+ cells) and H3T3A-expressing midguts  (avg. single Dl+ cells = 50.4% + 1.5%, N = 5 midguts, n = 2,522 Dl+ cells). Bars represent mean proportion of single Dl+ ISCs  out of all ISCs  counted. Error bars represent SEM. There are fewer single Delta-positive cells in H3T3A-expressing midguts  compared to H3-expressing midguts  (two sample t test, *** p &lt; 0.001)."
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(M) Comparison of the  mitotic index in H3-expressing (N = 8 midguts) and  H3T3A-expressing (N = 8 midguts) midguts. Total number of mitotic ISCs  were quantified per midgut  by the presence of H3S10 phosphorylation  mark. H3T3A-expressing midguts had a significantly higher mitotic index  when compared to H3-expressing midguts  (two sample t test, * p &lt; 0.05). Bars represent mean number of mitotic  cells per midgut. Error bars represent SEM."
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(A) A view of the midgut  of an UAS-H3-mCherry  transgene driven by esg-Gal4, Su(H)-Gal80, tub-Gal80ts at the Gal80ts permissive temperature  (18°C), showing no expression  of the histone  transgene or the UAS-eYFP   reporter that marks ISCs. (B) A view of the midgut  of an UAS-H3-mCherry  transgene driven by esg-Gal4, SuH-Gal80, tub-Gal80ts at the  Gal80ts restrictive temperature  (29°C), showing expression  of the H3-mCherry  transgene (red) and the UAS-eYFP  reporter (green). (C) A view of the midgut  of an UAS-H3T3A-mCherry  transgene driven by esg-Gal4, SuH-Gal80, tub-Gal80ts at the Gal80ts permissive temperature  (18°C), showing no expression  of the histone  transgene or the UAS-eYFP  reporter. (D) A view of the midgut  of an UAS-H3T3A-mCherry  transgene driven by esg-Gal4, SuH-Gal80, tub-Gal80ts at the Gal80ts restrictive temperature  (29°C), showing expression  of the H3T3A-mCherry  transgene (red) and the UAS-eYFP  reporter (green). Data information: Scale bars in (A), (B), (C), and (D): 50 µm"
NCBI gene IDs:
"mCherry: 
esg: 34903
Gal4: 855828
Gal80: 854954
H3: 3771723
Su(H): 34881
SuH: 34881
tub: 40554"
Figure legend: "(I) Comparison of the mitotic index  in H3-expressing (N = 11 midguts) and H3T3A-expressing (N = 11 midguts) midguts. Total number of mitotic ISCs  were quantified per midgut  by the presence of H3S10 phosphorylation  mark. Bars represent mean number of mitotic  cells per midgut. Error bars represent SEM. H3T3A-expressing midguts  had a significantly higher mitotic index when compared to H3-expressing midguts  (two sample t test, * p &lt; 0.05)."
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(J) Qualitative analysis of midgut  phenotypes. No phenotype is called when the ISCs  appear normally distributed based on eYFP  reporter signal. A mild phenotype is called when there is a small region of eYFP-positive cells clustered within a region, showing mild disorganization. A strong phenotype is called when there is a large region of eYFP-positive cells clustered, showing significant disorganization. (K) Percentage of H3- expressing midguts  (N = 11 midguts) and H3T3A- expressing midguts  (N = 11 midguts) with no phenotype, a mild phenotype, or a strong phenotype. Data information: Scale bars in (J): 500 µm."
NCBI gene IDs:
"H3: 3771723"
Figure legend: "(A) A view of the midgut  of an UAS-H3-mCherry  transgene driven by esg-Gal4, SuH-Gal80, tub-Gal80ts at the Gal80ts restrictive temperature (29°C), showing expression  of the H3-mCherry  transgene (grey) and the UAS-eYFP  reporter (green) from the driver as well as expression  of the Su(H)-LacZ  reporter line (magenta). eYFP  and Su(H)-LacZ  signals are organized and separable. (B) A view of the midgut  of an UAS-H3T3A-mCherry  transgene driven by esg-Gal4, SuH-Gal80, tub-Gal80ts at the Gal80ts restrictive temperature (29°C), showing expression  of the H3T3A-mCherry  transgene (grey) and the UAS-eYFP  reporter (green) from the driver as well as expression  of the Su(H)-LacZ  reporter line (magenta). eYFP  and  Su(H)-LacZ  signals are disorganized and overlapping. Data information: Scale bars in (A) and (B): 50 µm."
NCBI gene IDs:
"mCherry: 
esg: 34903
Gal4: 855828
Gal80: 854954
H3: 3771723
SuH: 34881
tub: 40554"
Figure legend: "(I) Wild-type and Cd22-/- primary B cells were treated with 5 µg/mL anti-kappa antibody or 1 µM LatA and intracellular calcium flux was measured by flow cytometry."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(J) Wild-type and Cd22Y2,5,6F primary B cells were treated with 5 µg/mL anti-kappa antibody or 1 µM LatA and intracellular calcium flux was measured by flow cytometry."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(K and L) Wild-type, Cd22-/- and Cd22Y2,5,6F primary B cells were treated with vehicle control (-) or 1 µM LatA for the indicated time. Cells were lysed and analysed by SDS-PAGE followed by immunoblotting with phospho-CD19 (pCD19), phospho-Akt (pAkt), phospho-ERK (pERK) and actin as loading control. The intensity of phosphorylated proteins, normalized to actin, was referred to the unstimulated sample of the wild type cells, set as 1."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(A-B) Wild-type and CD45-deficient primary B cells were stained with Alexa647conjugated antibody against CD22 and settled onto nonstimulatory coverslips. Cells were then fixed, imaged with dSTORM and analysed.(A) 2D (top) and pseudo 3D (bottom) dSTORM images were reconstructed from single molecule localizations. The white dashed square is shown in pseudo 3D (bottom).(B) Error bars (Hopkins index) and thin lines (H function) denote mean ± SEM. Data are pooled from three experiments."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(C-E) Wild-type and CD45-deficient primary B cells were labelled with Atto633-conjugated Fab fragments against CD22 or IgM, settled onto nonstimulatory coverslips and imaged. Single particle trajectories were then analysed.(C) Trajectories of CD22 in wild-type (left) and CD45-deficient cells (right) showing diffusion of single particles over 6 s.(D) Diffusion coefficients of 300 representative CD22 and IgM particles. Bars and numbers indicate the median. Data are pooled from three experiments.(E) Confinement analysis of CD22 and IgM. Bars and numbers indicate the median."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(F) Two-population analysis of CD22 diffusion. Diffusion coefficient were plotted on a logarithmic scale and fitted to two Gaussian-shaped curves to account for slower (light grey) and faster (dark grey) diffusing populations. Wild-type (blue line and circles, left) and CD45-deficient (blue line and squares, left middle) primary B cells were compared in an overlay (right middle) and their proportions plotted (right)."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(G and H) CD45-deficient primary B cells were labelled with Atto633-conjugated Fab fragments against CD22 or IgM, settled onto nonstimulatory coverslips, treated with vehicle control (DMSO) or 1µM LatA and imaged. Single particle trajectories were then analysed and diffusion coefficients calculated.(G) Diffusion coefficients of 300 representative CD22 and IgM particles. Bars and numbers indicate the median.(H) Confinement analysis. Bars and numbers indicate the median."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(I and J) CD45-deficient primary B cells were stained with Alexa 647-conjugated antibody against CD22, settled onto nonstimulatory coverslips and treated with vehicle control or sialidase. Cells were then fixed, imaged with dSTORM and analysed.(I) 2D (top) and pseudo 3D (bottom) dSTORM images were reconstructed from single molecule localizations. The white dashed square is shown in pseudo 3D (bottom).(J) Quantification of the distribution of CD22 with Hopkins index and H function. Error bars (Hopkins index) and thin lines (H function) denote mean ± SEM.Data are pooled from two experiments.*P&lt;0.05, **P&lt;0.001, ***P&lt;0.0001 (Student's t-test)."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(K and L) Wild-type and CD45-deficient primary B cells were treated with vehicle control (-) or 1 µM LatA for the indicated times. Cells were lysed and analysed by SDS-PAGE followed by immunoblotting with phospho-CD22 (p-CD22) and total CD22 as loading control. The intensity of p-CD22, normalized to CD22, was referred to the unstimulated sample of the wild-type cells, set as 1."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "(A-E) Wild-type and Cd22R130E primary B cells were labelled with Atto 633-conjugated Fab fragments against CD22 or IgM, settled onto nonstimulatory coverslips and imaged. Single particle trajectories were then analysed.(A) Trajectories of CD22 in Wild-type (left) and Cd22R130E cells (right) showing diffusion of single particles over 6 s.B) Diffusion coefficients of 300 representative CD22 and IgM particles. Bars and numbers indicate the median.(C) Confinement analysis. Bars and numbers indicate the median.(D and E) Two-population analysis of CD22 diffusion. Analysis of CD22 diffusion plotted on a logarithmic scale and fitted to two Gaussian-shaped curves to account for slower and faster diffusing populations.(D) Wild-type (blue line and circles) and Cd22R130E (blue dotted line and squares middle) primary B cells were compared in an overlay (left) and the proportions of the faster diffusing populations plotted (right).(E) Proportions of faster-diffusing populations."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(F and G) Wild-type and Cd22R130E primary B cells were stained with Alexa 647-conjugated antibody against CD22 and settled onto nonstimulatory coverslips. Cells were then fixed, imaged with dSTORM and analysed.(F) 2D (top) and pseudo 3D (bottom) dSTORM images were reconstructed from single molecule localizations. The white dashed square is shown in pseudo 3D (bottom).(G) Quantification of the distribution of CD22 with Hopkins index and H function. Error bars (Hopkins index) and thin lines (H function) denote mean ± SEM. Data are pooled from three experiments."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(H) Wild-type and Cd22R130E primary B cells were treated with 1 µM LatA and intracellular calcium flux was measured by flow cytometry."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "(I-K) Wild-type and Cd22R130E primary B cells were treated with vehicle control (-) or 1 µM LatA for the indicated time. Cells were lysed and analysed by SDS-PAGE followed by immunoblotting with phospho-CD19 (p-CD19), phospho-Akt (pAkt), phospho-ERK (pERK) and actin and total ERK as loading controls. The intensity of phosphorylated proteins, normalized to actin or ERK, was referred to the unstimulated sample of the wild type cells, set as 1.Data are representative of at least two experiments."
NCBI gene IDs:
"Cd22: 12483"
Figure legend: "Slices through electron cryo-tomograms showing fully-assembled motors without the hook and filament. The dashed-yellow circles indicate the IM sub-complex. Schematic representation of the P. aeruginosa motors lacking the hook and filament shown in (A-C). Slices through electron cryo-tomograms showing fully-assembled motors with the hook and lacking the filament. Schematic representation of the motors with the hook shown in (E-G). Slices through electron cryo-tomograms of intact P. aeruginosa ∆flgI cells showing the presence of the inner-membrane sub-complex with the rod. Schematic representation of the inner-membrane sub-complex with the rod shown in (I-K). Slices through electron cryo-tomogram of lysed P. aeruginosa ∆flgI cells showing the presence of the sub-complex constituting the MS-ring and the rod. Schematic representation of the sub-complex described in (M-O). A slice through an electron cryo-tomogram of a P. aeruginosa ∆flgG cell highlighting the presence of the inner-membrane sub-complex. Schematic representation of the inner-membrane sub-complex shown in (Q)."
NCBI gene IDs:
"flgG: 880935
flgI: 878536"
Figure legend: "Slices through electron cryo-tomograms of wild type cells showing fully-assembled motors without the hook and filament. Schematic representation of the motors lacking the hook and filament shown in (A-C). Sub-tomogram average of the motors of fully-assembled flagella. Slice through an electron cryo-tomogram of a ΔflgH cell showing an IM sub-complex, indicated by the dashed-yellow circle. Schematic representation of the IM sub-complex shown in (F). Slices through electron cryo-tomograms of ΔflgH cells showing the IM sub-complex with the rod and the P-ring, indicated by the dashed-yellow circles. Schematic representation of the structures shown in (H and I). Slices through electron cryo-tomograms of ΔflaA/B cells highlighting the flagellar motor and the hook (without the filament). Sub-tomogram average of the flagellar motor and the hook structure found in ΔflaA/B cells. Schematic representation of the sub-tomogram average shown in (N). Slices through electron cryo-tomograms of ΔflaA/B cells illustrating a disassembly product constituting the PL sub-complex, the rod and the hook. Sub-tomogram average of the disassembly complex shown in (P-R). The dashed-orange arrow indicates the absence of the IM sub-complex in this structure. Schematic representation of the disassembly product shown in (P-S). Slices through electron cryo-tomograms of ΔflaA/B cells highlighting PL sub-complexes (dashed-yellow circles). Sub-tomogram average of the PL sub-complexes in ΔflaA/B cells. Schematic representation of the sub-tomogram average shown in (X)."
NCBI gene IDs:
"flaA: 1170946
flgH: 1170937"
Figure legend: "K) Western blot of Ub-K48 proteins in the insoluble fraction of protein lysates of SOD1-G93A mouse spinal cord plus or minus heat shock. L) Quantification of panel K. Each central line and error bar indicate mean +/- SEM of a biological replicates (n=5 biological replicates per group) Data information: * indicates p&lt;0.05, ** indicates p&lt;0.005, and *** indicates p&lt;0.0005 by unpaired two tailed students t-test comparing male and female samples or samples with or without heat shock."
NCBI gene IDs:
"SOD1: 20655"
Figure legend: "M) Chymotrypsin-like proteasome activity in spinal cord lysates from SOD1-G93A mice. Each central line and error bar indicate mean +/- SEM of a biological replicates (n=5 biological replicates per group). Bordered points indicate biological replicates and un-bordered points indicate technical replicates. Data information: * indicates p&lt;0.05, ** indicates p&lt;0.005, and *** indicates p&lt;0.0005 by unpaired two tailed students t-test comparing male and female samples or samples with or without heat shock."
NCBI gene IDs:
"SOD1: 20655"
Figure legend: "N) Filter trap assay of protein lysates from the insoluble fraction of SOD1-G93A spinal cord lysates plus or minus heat shock. O) Quantification of panel N. Each central line and error bar indicate mean +/- SEM of a biological replicates (n=5 biological replicates per group) Data information: * indicates p&lt;0.05, ** indicates p&lt;0.005, and *** indicates p&lt;0.0005 by unpaired two tailed students t-test comparing male and female samples or samples with or without heat shock."
NCBI gene IDs:
"SOD1: 20655"
Figure legend: "P) Representative transmission electron micrographs of protein aggregates in the insoluble fraction of SOD1-G93A spinal cord lysates plus or minus heat shock. Scale bar equals 600nm. Q) Quantification of P. (n=9 technical replicates per group) Data information: * indicates p&lt;0.05, ** indicates p&lt;0.005, and *** indicates p&lt;0.0005 by unpaired two tailed students t-test comparing male and female samples or samples with or without heat shock."
NCBI gene IDs:
"SOD1: 20655"
Figure legend: "FLMycER cells, primed or not with OHT  (48h), were treated with 135 nm  IACS-010759 (IACS) for 48 hours (B) H2O2  quantification, expressed as fold-increase of the 405/488 nm fluorescence ratio in treated vs. untreated  FLMycER cells, expressing either the cytoplasmic  (left) or mitochondrial  (right) roGFP2-ORP1  biosensor. Data information: * p ≤ 0.05 (one-way ANOVA). Each point in the graphs in B from an independent biological replicate, each representing the average of thousands of events (single cells) in a distinct cell population, normalized to the untreated condition."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "FLMycER cells, primed or not with OHT  (48h), were treated with 135 nm IACS-010759 (IACS) for 48 hours (C) Superoxide anion O2•-  production in treated vs. untreated FLMycER cells, based on dihydroethidium staining. Data information: * p ≤ 0.05 (one-way ANOVA). Each point in the graphs in B and C is from an independent biological replicate, each representing the average of thousands of events (single cells) in a distinct cell population, normalized to the untreated condition."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "FLMycER cells were primed with100 nM OHT  (48h) and/or treated with 135nM IACS-010759, as indicated. (B) glutathione  quantification in FLMycER cells, measured after 40 hours of IACS-010759 treatment. Data information: n=3 biological replicates; error bars: SD. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 (one-way ANOVA)."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "(F) Immunoblot  on lysates from FLMycER cells treated as indicated, with the addition of 10 mM NAC  concomitantly with IACS-010759."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "FLMycER  cells were primed with 100 nM OHT  and treated with 135 nM  IACS-010759, as indicated. (B) NADPH/NADP redox state in  FLMycER cells, assayed after 40 hours of IACS-010759 treatment. n=3 biological replicates. ** p ≤ 0.01; **** p ≤ 0.0001 (one-way ANOVA)."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "FLMycER cells were primed with 100 nM OHT  and treated with 135 nM  IACS-010759, as indicated. (E-G) Cell viability after 40 hours of IACS-010759 treatment in the presence of (E) 50 µM dehydroepiandrosterone  (DHEA), (F) 10 µM 6- aminonicotinamide  (6AN) or (G) 1 mM  2-deoxyglucose (2DG). See Supplemental Figure3F for detailed statistical analysis. Data information: In E,G) n=6; in (F) n=9; all biological replicates; error bars: SD."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "(A) Viability  of FLMycER and BaFMycER cells primed or not with 100 nM OHT  and treated with 135 nM IACS-010759 for 48h and/or with ascorbate  at the indicated concentration for 6h. n=3 biological replicates; error bars: SD."
NCBI gene IDs:
"Myc: 17869"