EMBO/SourceData huggingface dataset:
Figure legend: "Quantitative analysis of capillary numbers. GLS-positive capillaries were counted in wild-type (wt) and Dnmt2−/− (D2-) mice, in three independent high-power fields (HPF) per mouse (n = 7)."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Representative FACS plots of wild-type and Dnmt2−/− BM stained for Lin− Sca1+ and cKit+ cells."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Quantification of bone marrow LSK cells in wild-type (three independent experiments, each with pooled BM samples from 4 to 6 mice) and Dnmt2−/−bone marrow (three independent experiments, each with pooled BM samples of 5-9 mice)."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Restoration of the LSK cell population in adult Dnmt2−/− mice. Frequencies of LSK cells were normalized to the frequencies of LSK cells from the bone marrow of wild-type littermates at the indicated age (2-3 independent experiments, each with pooled bone marrow samples from 3 to 9 mice)."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Increased LSK CD34− and reduced LSK CD34+cell populations in 8-day- and 1-year-old Dnmt2−/− mice compared to wild-type (two independent experiments, each with pooled bone marrow samples from 3-9 mice)."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "qPCR analysis of Dnmt2 mRNA expression in haematopoietic tissues, normalized to GAPDH."
NCBI gene IDs:
"GAPDH: 
Dnmt2: 13434"
Figure legend: "Proliferation analysis of primary bone marrow suspension cells. Primary bone marrow suspension cells isolated from the femurs of 8-day-old mice were grown together with the attached stromal cells. Dnmt2−/− population doubling levels of three biological replicates, each plated three times, were calculated relative to wild-type culture."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Reduced replating abilities of primary Dnmt2−/−bone marrow cells in a myeloid colony formation assay. For primary (1°) colony formation, 1.25 × 104 total bone marrow cells were plated in semisolid medium allowing myeloid differentiation. For secondary (2°) colony formation, cells were harvested and 0.1% of primary colonies were replated into semisolid medium. Results are from two independent experiments analyzing 9 wild-type and 13 Dnmt2−/−mice in total."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Reduced secondary CFU-preB colony formation of Dnmt2−/− cells. Numbers of CFU-preB-cell colonies are indicated per 5 × 104 wild-type or Dnmt2−/− total bone marrow cells. 10% of primary colonies were replated into secondary CFU-preB assays."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Stable engraftment (upper panel) and multi-lineage reconstitution (lower panel) of wild-type and Dnmt2−/− bone marrow transplants. CD11b: macrophages; Ly6G: granulocytes; CD45R: B cells; CD3: T cells."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Dnmt2 expression after 3 days of mesenchymal cell differentiation."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Alizarin Red staining, indicating enhanced osteoblast differentiation of primary stromal cells from Dnmt2−/− mice. Scale bar: 50 μm. Quantification was performed with three biological replicates."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "qPCR analysis of the osteoblast markers Osterix and Osteocalcin."
NCBI gene IDs:
"Osteocalcin: 12096
Osterix: 170574"
Figure legend: "Oil Red O/hematoxylin staining, showing reduced adipocyte differentiation of primary stromal cells from Dnmt2−/− mice. Scale bar: 50 μm. Quantification was performed with three biological replicates."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "qPCR analysis of the adipocyte markers CEBp alpha and Glut4."
NCBI gene IDs:
"CEBp alpha: 12606
Glut4: 20528"
Figure legend: "Representative Northern blot showing tRNA(Gly) and tRNA(Asp) fragmentation in bone marrow from 8-day-old and adult mice. tRNA(Ser) was included as a Dnmt2 non-substrate tRNA, and 5S rRNA was included as a loading control."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "tRNA fragment coverage for the Dnmt2 substrates tRNA(Gly) and tRNA(Asp), and the non-substrate tRNA(Ser) in bone marrow, as determined by deep sequencing. Wild-type coverage is represented in red, and Dnmt2−/− coverage is indicated in blue. The plots illustrate the specific increase of 5′-halves from tRNA-Gly and 3′-halves from tRNA-Asp fragments in Dnmt2 mutants."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Analysis of protein synthesis by dynamic SILAC. The top 10% of proteins that are translated slower in Dnmt2−/− versus wild-type cells are indicated in orange. The top 10% of proteins that are translated faster in Dnmt2−/− versus wild-type cells are shown in blue. A change of &gt;  twofold is indicated by the black lines."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Dimethyl-labeling analysis of 8-day-old mouse bone marrow tissue. Proteins that were down-regulated (&lt; twofold change) in Dnmt2-deficient cells are indicated in red, and up-regulated proteins (&gt; twofold change) are shown in green. Nestin and periostin are marked with arrows."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Correlation between protein and mRNA expression for differentially expressed proteins. Proteins that were down-regulated (&lt; twofold change) in Dnmt2-deficient cells are indicated in red, and up-regulated proteins (&gt; twofold change) are shown in green. The Pearson correlation coefficient is −0.03."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Representative staining of nestin and periostin in femur sections from 8-day-old wild-type and Dnmt2−/− mice. The anti-periostin staining is comparable in the periosteum of wild-type and Dnmt2−/− mice, but is reduced in the bone marrow cells (compare the highlighted areas). Scale bar: 100 μm. Nuclei are counterstained with DAPI (blue)."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "Quantitative differences in the length of the nestin-positive trabecular zone (upper panel) between wild-type (30 measurements, three mice) and Dnmt2−/− (21 measurements, two mice) and in bone periostin-positive marrow areas (lower panel) between wild-type (26 measurements, three mice) and Dnmt2−/− (45 measurements, three mice) in femur sections from 8-day-old mice. Data are presented as mean ± SD."
NCBI gene IDs:
"Dnmt2: 13434"
Figure legend: "(E) Immunoblot (IB) analysis of expression of IFT74, IFT81, RabL2B and α-tubulin in control (sgSafe) or IFT74 KO RPE cells stably expressing either empty vector, FLAG-IFT74, or FLAG-IFT74 T438R. Representative images from three experimental replicates are shown. The cells were grown to confluence (without serum starvation), lysed and analyzed by Immunoblot. Molecular weights (kDa) estimated from a protein marker are indicated. Red asterisk: endogenous IFT74; black asterisks: FLAG tagged IFT74."
NCBI gene IDs:
"FLAG: 
IFT74: 80173"
Figure legend: "(G-I) Box plots showing centrosomal signal intensity of FLAG (G), IFT81 (H), or RabL2 (I) in the cells described in (D) serum starved for 24 hours. The relative fluorescence signal intensity compared with the average of the control (IFT74 KO + FLAG-IFT74 WT for (G) and sgSafe + empty for (H) and (I)) are shown. Data are combined from three experimental replicates. Statistics obtained through nested one-way ANOVA with Šídák's multiple comparison test. (G) The number of mother centrioles analyzed in each sample per replicate are: 78, 77, 78 (sgSafe + empty); 102, 95, 101 (IFT74 KO + empty); 54, 77, 85 (IFT74 KO + FLAG-IFT74 WT); 49, 83, 99 (IFT74 KO + FLAG-IFT74 T438R), (H) The number of mother centrioles analyzed in each sample per replicate are: 93, 137, 138 (sgSafe + empty); 109, 133, 193 (IFT74 KO + empty); 62, 146, 131 (IFT74 KO + FLAG-IFT74 WT); 41, 143, 133 (IFT74 KO + FLAG-IFT74 T438R), (I) The number of mother centrioles analyzed in each sample per replicate are: 64, 101, 106 (sgSafe + empty); 84, 105, 129 (IFT74 KO + empty); 52, 96, 121 (IFT74 KO + FLAG-IFT74 WT); 73, 92, 102 (IFT74 KO + FLAG-IFT74 T438R)."
NCBI gene IDs:
"FLAG: 
IFT74: 80173"
Figure legend: "(K) Box plots showing the quantification of number of IFT88 particles per cilium in the experiment described in (J). Data are combined from three experimental replicates: The number of cilia analyzed in each sample per replicate are: 20, 20, 18 (sgSafe + empty); 20, 20, 18 (IFT74 KO + WT); 20, 20, 19 (IFT74 KO + FLAG-IFT74 WT). The number of IFT particles in individual cells in each experiment are available from Figures S7A, D, and G. Statistics obtained through nested one-way ANOVA with Tukey's multiple comparison test."
NCBI gene IDs:
"FLAG: 
IFT74: 80173"
Figure legend: "Time-lapse fluorescence microscopy recordings showing localization and dynamics of the sfGFPTssM fusion protein in the parental (WT) and TssJ mutated strains (TssJ D97K). Individual images were taken every 60 seconds. The corresponding time of each micrograph is indicated in the lower right part of the image. Stable foci in the WT strain and less stable foci in the TssJ D97K strain are respectively indicated by white and blue arrowheads. Scale bars, 1 µm."
NCBI gene IDs:
"TssJ: "
Figure legend: "Fluorescence microscopy recordings showing sfGFPTssM foci in the parental (WT) and TssM mutated strains (TssM Q779C/N780C, TssM ∆777-783). TssM foci containing cells are indicated by arrowheads. Microscopy analyses were performed independently three times, each in technical triplicate, and a representative experiment is shown. Scale bars, 1 µm."
NCBI gene IDs:
"TssM: "
Figure legend: "Fluorescence microscopy recordings showing TssBsfGFP sheath in the parental (WT) and TssM mutated strains (TssM Q779C/N780C, TssM ∆777-783). Fluorescent sheath containing cells are indicated by arrowheads. Microscopy analyses were performed independently three times, each in technical triplicate, and a representative experiment is shown. Scale bars, 1 µm."
NCBI gene IDs:
"TssM: "
Figure legend: "(A) RT-qPCR quantification of relative miR-21-3p and miR-21-5p levels in mouse brain, heart, kidney and epidermis. N=4 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"miR-21-3p: 102466637///387140///102465212
miR-21-5p: 102465212///102466637///387140"
Figure legend: "(B) Fluorescent miR-21-3p in situ hybridization (pink) in dorsal skin of acutely irradiated (Ac-UV) and non-irradiated (no UV) Ppard +/+ and -/- mice. E: epidermis; D: dermis; HF: hair follicle. Magnification bar: 100m"
NCBI gene IDs:
"miR-21-3p: 102466637///387140///102465212
Ppard: 19015"
Figure legend: "(C) RT-qPCR quantification of relative pri-miR-21 and miR-21-3p levels in the epidermis (left and middle), and of relative miR-21-3p in the dermis (right) of acutely irradiated (Ac-UV; +) and non-irradiated (-) Ppard +/+ and -/- mice. Pri-miR-21: Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.022; miR-21-3p: Ppard +/+ no UV vs Ppard +/+ Ac-UV P = 0.017, Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.008. N=3-4 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"miR-21: 102465212///102466637///387140
miR-21-3p: 102465212///102466637///387140
Ppard: 19015"
Figure legend: "(D) RT-qPCR quantification of relative miR-21-3p levels in total skin of chronically irradiated (Chr-UV; +) and non-irradiated (-) Ppard +/+ and -/- mice. miR-21-3p: Ppard +/+ no UV vs Ppard +/+ Chr-UV P = 0.008, Ppard +/+ Chr-UV vs Ppard -/- Chr-UV P = 0.002, ns: non significant. N=4 animals per groups, one representative experiment is shown out of two independent replicates."
NCBI gene IDs:
"miR-21-3p: 102465212///102466637///387140
Ppard: 19015"
Figure legend: "(E) RT-qPCR quantification of relative miR-21-3p level in the epidermis of Ppard +/+ and -/-mice, acutely irradiated (Ac-UV; +) or non-irradiated (-), treated with the PPAR/ antagonist GSK0660 (+) or vehicle (-), as indicated. miR-21-3p: Ppard +/+ no UV vs Ppard +/+ Ac-UV P = 0.005, Ppard +/+ Ac-UV vs Ppard +/+ Ac-UV/GSK0660 P = 0.04, N=5 (Ppard +/+) to 3 (Ppard -/-) animals per group. One representative experiment is shown out of two independent replicates"
NCBI gene IDs:
"miR-21-3p: 102465212///102466637///387140
Ppard: 19015"
Figure legend: "(F) RT-qPCR quantification of relative miR-21-3p levels in HaCat cells treated with the PPAR/ agonists GW501516, GW0742 (+) or vehicle (-) as indicated. miR-21-3p: Veh vs GW501516 P = 4E-04, Veh vs GW0742 P = 2E-04. N=2-3 biological replicates, one representative experiment is shown out of two independent replicates."
NCBI gene IDs:
"miR-21-3p: 406991
miR-21-3p: 406991///387140"
Figure legend: "(A) RT-qPCR quantification of relative pri-miR-21 level in HaCaT human keratinocytes treated with the PPAR/ agonist GW0742 (+) or vehicle (-), with (+) or without (-) cycloheximide (Cyclo) as indicated. Pri-miR-21: Veh vs GW0742 P = 0.009. N=3 biological replicates, one representative experiment is shown out of two independent replicates."
NCBI gene IDs:
"miR-21: 406991"
Figure legend: "(B) RT-qPCR quantification of relative pri-miR-21, pre-miR-21, miR-21-5p and miR-21-3p levels in HaCaT cells treated for 24h with 2 or 5ng/ml of recombinant human TGFβ-1 (+) or vehicle (-) as indicated. Pri-miR-21: Veh vs TGF-1 5ng/ml P = 0.029; Pre-miR-21: Veh vs TGF-1 5ng/ml P = 0.001; miR-21-5p: Veh vs TGF-1 5ng/ml P = 0.002; miR-21-3p: Veh vs TGF-1 5ng/ml P = 1.7E-05. N=3 biological replicates, one representative experiment is shown out of two independent replicates ."
NCBI gene IDs:
"miR-21: 406991
miR-21-3p: 406991
miR-21-5p: 387140///102465212///102466637///406991"
Figure legend: "(C) RT-qPCR quantification of pri-miR-21, pre-miR-21, miR-21-5p and miR-21-3p levels in HaCat cells treated for 24h with the PPAR/ agonist GW0742 (+), TGFβ receptor inhibitor SB431542 (+), or vehicle (-) as indicated. Pri-miR-21: GW0742 vs SB431542 P = 0.004, GW0742 vs GW0742/SB431542 P = 0.015; Pre-miR-21: Veh vs GW0742 P = 0.022, GW0742 vs SB431542 P = 0.036, GW0742 vs GW0742/SB431542 P = 0.024; miR-21-5p: GW0742 vs SB431542 P = 0.034, GW0742 vs GW0742/SB431542 P = 0.024; miR-21-3p: Veh vs GW0742 P = 0.036, GW0742 vs SB431542 P = 0.011, GW0742 vs GW0742/SB431542 P = 0.008. N=3 biological replicates, one representative experiment is shown out of two independent replicates."
NCBI gene IDs:
"miR-21: 406991
miR-21-3p: 406991
miR-21-5p: 387140///102465212///102466637///406991"
Figure legend: "(D) RT-qPCR quantification of relative Tgfb1 level in the epidermis of acutely irradiated (Ac-UV) and non-irradiated Ppard +/+ and -/- mice. Tgfb1: Ppard +/+ no UV vs Ac-UV P= 0.034, Ppard +/+ Ac-UV vs Ppard Ac-UV P= 0.030. N=2-3 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"Ppard: 19015
Tgfb1: 21803"
Figure legend: "(E) RT-qPCR quantification of relative miR-21-5p and miR-21-3p levels in the skin of Ppard +/+ mice treated with the TGFβ receptor inhibitor SB431542 (+) or vehicle (-), with (+) or without (-) acute UV exposure (Ac-UV). miR-21-5p: no UV vs Ac-UV P = 0.038, Ac UV vs Ac UV/SB431542 P = 0.028; miR-21-3p: no UV vs Ac-UV P = 0.017; Ac-UV vs Ac-UV/SB431542 P = 0.035. N=3 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"miR-21-3p: 102466637///387140///102465212
miR-21-5p: 102466637///387140///102465212
Ppard: 19015"
Figure legend: "(A) RT-qPCR quantification of relative SMAD7 expression level in the epidermis of acutely irradiated (Ac-UV; +) ex vivo biopsies of human normal skin. SMAD7: no UV vs Ac-UV P = 0.032. N=4 biological replicates, one representative experiment is shown out of three independent experiments performed with the skin of three different donors."
NCBI gene IDs:
"SMAD7: 4092"
Figure legend: "(B) Left panel: wild-type (WT SMAD7 3'UTR) and mutated (MUT SMAD7 3'UTR; *: mutated nucleotides) miR-21-3p binding sequences in the humanSMAD7 3'UTR. Right panel: Luciferase reporter assay with wild-type (WT SMAD7 3'UTR) or mutated (MUT SMAD7 3'UTR) SMAD7 3'UTR in HEK293 cells overexpressing miR-21-3p (miR-21-3p mimic) or a scrambled sequence (Control)."
NCBI gene IDs:
"Luciferase: 
miR-21-3p: 406991
SMAD7: 4092"
Figure legend: "(C) Left panel: normalized expression data of SMAD7 mRNA obtained from genomic microarray analysis of human HaCaT cells treated with a miR-21-3p mimic (miR-21-3p mimic) or a scrambled sequence (control). N=3 biological replicates. Middle panel: western blot quantification of SMAD7 protein level (normalized to GAPDH protein level) in human HaCaT cells treated with a miR-21-3p mimic (miR-21-3p mimic) or a scrambled sequence (control). SMAD7: control vs miR-21-3p mimic P = 0.031. N=3 biological replicates, Right panel: western blot of SMAD7 and GAPDH proteins in human HaCaT cells treated with a miR-21-3p mimic (miR-21-3p mimic) or a scrambled sequence (control), N=3 biological replicates; one representative experiment is shown out of two independent replicates."
NCBI gene IDs:
"miR-21-3p: 406991
SMAD7: 4092"
Figure legend: "(D) Western blot of Smad7 from epidermis of acutely irradiated (Ac-UV; +) Ppard +/+ and -/- mice. Loading control: GAPDH."
NCBI gene IDs:
"Ppard: 19015"
Figure legend: "(E) RT-qPCR quantification of relative TGFB1, SERPINE1, p21, RUNX and SNAI2 levels in human HaCaT cells treated with a miR-21-3p mimic (miR-21-3p mimic) or a scrambled sequence (control) with (+) or without (-) treatment with 2ng/ml recombinant TGFβ-1. SERPINE1: TGF-1 2ng/ml control vs miR-21-3p mimic P = 0.004; p21: TGF-1 2ng/ml control vs miR-21-3p mimic P =0.03; RUNX: TGF-1 2ng/ml control vs miR-21-3p mimic P = 0.04. N=3 biological replicates."
NCBI gene IDs:
"p21: 1026
miR-21-3p: 406991
RUNX: 864///860///861
SERPINE1: 5054
SNAI2: 6591
TGFB1: 7040"
Figure legend: "(C) RT-qPCR analysis of IL6, IL1B, IL1RAP, PTGS2, CCL5, CXCL10 and CASP14 levels in HaCat cells transfected with a miR-21-3p mimic or a scrambled sequence (control). IL6: control vs miR-21-3p mimic P = 0.05; IL1B: control vs miR-21-3p mimic P = 0.005; IL1RAP: control vs miR-21-3p mimic P = 0.008; PTGS2: control vs miR-21-3p mimic P = 0.050; CCL5: control vs miR-21-3p mimic P = 0.001; CXCL10: control vs miR-21-3p mimic P = 0.012; CASP14: control vs miR-21-3p mimic P = 9E-05. N=3 biological replicates, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"CASP14: 23581
CCL5: 6352
CXCL10: 3627
IL1B: 3553
IL1RAP: 3556
IL6: 3569
miR-21-3p: 406991
PTGS2: 5743"
Figure legend: "(A) RT-qPCR quantification of Il6, Il1b, Ptgs2 and Ccl5 levels in the epidermis of acutely irradiated (Ac-UV; +) and non-irradiated (-) Ppard +/+ and -/- mice. Il6: Ppard +/+ no UV vs Ac-UV P = 7E-04, Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.008; Il1b: Ppard +/+ no UV vs Ac-UV P = 0.010, Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.016, Ppard -/- no UV vs Ac-UV P = 0.015; Ptgs2: Ppard +/+ no UV vs Ac-UV P = 0.028, Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.028, Ppard -/- no UV vs Ac-UV P = 8E-04; Ccl5: Ppard +/+ no UV vs Ac-UV P = 0.002, Ppard +/+ Ac-UV vs Ppard -/- Ac-UV P = 0.014, Ppard -/- no UV vs Ac-UV P = 0.010; ns: non significant. N=4 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"Ccl5: 20304
Il1b: 16176
Il6: 16193
Ppard: 19015
Ptgs2: 19225"
Figure legend: "(B) Left panel: Immunostaining for infiltrating F4/80-positive macrophages in acutely irradiated (Ac-UV) and non-irradiated (no UV) Ppard +/+ and -/- miceskin sections. Magnification bar: 200m. Right panel: RT-qPCR quantification of relative levels of the macrophage marker Emr1 mRNA in total skin of acutely irradiated (Ac-UV; +) and non-irradiated (-) Ppard +/+ and -/- mice. Emr: Ppard +/+ no UV vs Ac UV P = 0.034; Ppard +/+ Ac UV vs Ppard -/- Ac UV P = 0.033. N=4 animals per group, one representative experiment is shown out of three independent replicates."
NCBI gene IDs:
"Emr1: 13733
Ppard: 19015"
Figure legend: "(C) RT-qPCR quantification of relative levels of Il6, Il1b, Ptgs2 and Tnfa in mouseepidermis acutely irradiated (Ac-UV; +) and non-irradiated (-), treated with miR-21-3p inhibitor (+) or mismatched control (-) as indicated."
NCBI gene IDs:
"Il1b: 16176
Il6: 16193
miR-21-3p: 102465212///102466637///387140
Ptgs2: 19225
Tnfa: 21926"
Figure legend: "(A) RT-qPCR quantification of relative levels of pri-miR-21, pre-miR-21, miR-21-5p and miR-21-3p levels in healthy human skin and human squamous cell carcinomas (SCC). pri-miR-21 Healthy skin vs SCC P = 0.043; pre-miR-21 Healthy skin vs SCC P = 3E-05; miR-21-5p Healthy skin vs SCC P = 7E-05; miR-21-3p Healthy skin vs SCC P = 1.4E-06. ≥ 5 independent biopsies per condition."
NCBI gene IDs:
"miR-21: 406991///102465212///102466637///387140
miR-21-3p: 406991///102465212///102466637///387140
miR-21-5p: 406991///102465212///102466637///387140"
Figure legend: "(B) RT-qPCR quantification of relative levels of pri-miR-21, pre-miR-21, miR-21-5p and miR-21-3p levels in healthy human skin and humanpsoriasis lesions. pri-miR-21 Healthy skin vs Psoriasis P = 0.034; miR-21-5p Healthy skin vs Psoriasis P = 0.034; miR-21-3p Healthy skin vs Psoriasis P = 0.036; ns: non significant, ≥ 4 independent biopsies per condition."
NCBI gene IDs:
"miR-21: 406991
miR-21-3p: 406991///102465212///102466637///387140
miR-21-5p: 406991///102465212///102466637///387140"
Figure legend: "(C) RT-qPCR quantification of relative levels of IL6, IL1B, PTGS2 and TNFA levels in epidermis of abdominal healthy humanskin biopsies exposed to acute UV ex vivo, with or without topical treatment with miR-21-3p inhibitor (+) or mismatched control (-) as indicated. ns: not significant. IL6: no UV vs Ac-UV P = 0.036, Ac-UV vs Ac-UV/miR-21-3p inhibitor P = 0.019; IL1B: no UV vs Ac-UV P = 0.002, Ac-UV vs Ac-UV/miR-21-3p inhibitor P = 0.023; PTGS2: no UV vs Ac-UV P = 0.008, Ac-UV vs Ac-UV/miR-21-3p inhibitor P = 0.022; TNFA: no UV vs Ac-UV P = 0.032, ns = non significant. ≥ 7 replicates, a pool of three independent experiments performed with the skin of three different donors is shown."
NCBI gene IDs:
"IL1B: 3553
IL6: 3569
miR-21-3p: 406991///102465212///102466637///387140
PTGS2: 5743
TNFA: 7124"
Figure legend: "Western blot analysis showing increased RNF8 protein level in HeLa cells after siRNA-mediated p97 depletion under physiological conditions and after IR (10 Gy).   Graph represents the quantifications of (A) (***P&lt;0.001; unpaired t-test, n=3, mean +SEM).   "
NCBI gene IDs:
"p97: 7415"
Figure legend: "Western blot analysis showing increased RNF8 protein level in HEK293 cells after doxycycline-induced mild expression of the p97EQ variant under physiological conditions and after IR (10 Gy).   Graph represents the quantifications of (E) (**P&lt;0.01, ****P&lt;0.0001; unpaired t-test, n=4, mean +SEM).   "
NCBI gene IDs:
"p97: 7415"
Figure legend: "Western blot analysis of CHX chase kinetics showing reduced RNF8 degradation rate in HEK293 cells after siRNA-mediated p97 depletion.   Graph represents the quantifications of (H) (***P&lt;0.001, ****P&lt;0.0001; two-way ANOVA, n=3, mean +SEM) and Western blot for efficacy of siRNA depletion of p97 (right).   "
NCBI gene IDs:
"p97: 7415"
Figure legend: "Western blot analysis of Flag-RNF8 denaturing-IP in HEK293 cells showing K48-linked hyper-ubiquitination of RNF8 after siRNA-mediated p97 depletion."
NCBI gene IDs:
"p97: 7415"
Figure legend: "Western blot analysis of Strep-p97 Co-IP in HEK293 cells showing increased RNF8 interaction with p97EQ variant as compared to p97WT under physiological conditions and after IR (10 Gy).   Graph represents the quantifications of (K) (*P&lt;0.05; unpaired t-test, n=2, mean +SEM).   "
NCBI gene IDs:
"p97: 7415"
Figure legend: "Western blot analysis of Flag-RNF8 denaturing-IP in HEK293 cells showing the ubiquitination pattern of RNF8-WT and RNF8-RING* variant, under siRNA-mediated luciferase (siLuc) or p97-depleted conditions (sip97)."
NCBI gene IDs:
"Luc: 
luciferase: 
RNF8: 9025
p97: 7415"
Figure legend: "Western blot analysis of CHX chase kinetics in U2OS cells, comparing the degradation rate of Flag-RNF8-WT and Flag-RNF8-RING*. Endogenous RNF8 was depleted by shRNF8 targeting only endogenous RNF8.   Graph represents the quantifications of (E) (ns P&gt;0.05, ****P&lt;0.0001; two-way ANOVA, n=3, mean +SEM).   "
NCBI gene IDs:
"Flag: 
RNF8: 9025"