EMBO/SourceData huggingface dataset:
Figure legend: "(B,C) Parvalbumin immunoreactivity in PV+ interneurons was significantly decreased in the somatosensory cortex of Cox6a2-/- mice compared to wild-types (n=4 WT, 4 Cox6a2-/-; 2-month-old). C: two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(D) In Cox6a2-/- mice, cortical PV+ interneurons showed a fewer number of synapses innervating principal neurons (n=3 WT, 3 Cox6a2-/-; 2-month-old). Two-tailed unpaired Student's t-test."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(E) To analyze changes in oxidative stress in PV+ interneurons, the somatosensory and motor cortices of WT and Cox6a2-/- mice were stained with an antibody against 8-OHdG, a marker of oxidative DNA damage. (F) 8-OHdG immunoreactivity was significantly higher in PV+ null in both cortices in Cox6a2-/- mice (n=4 WT, 4 Cox6a2-/-; 2-month-old). Two-tailed unpaired Mann-Whitney tests. "
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(G) The density of PNNs around PV+ interneurons residing in the somatosensory and motor cortices was analyzed by measuring the immunoreactivity of WFA that stains PNNs. (H) WFA immunoreactivity around PV+ cells was lower in both cortices in Cox6a2-/- mice (n=4 WT, 4 Cox6a2-/-; 2-month-old). Two-tailed unpaired Mann-Whitney tests. "
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(A) PV+ interneurons were sorted from cortices of PV-EGFP WT and Cox6a2-/- mice at P15 and processed for bulk RNA sequencing (n= 4 WT, 6 Cox6a2-/-; P15). Heatmap shows expression for top down- (red) and upregulated (blue) genes in Cox6a2-/- PV+ interneurons relative to their WT counterparts."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(B) Cox6a2 expression was effectively abolished in Cox6a2-/- PV+ interneurons. In addition, Cox6a2 knockout led to a decreased expression of Gad1 and Gad2 genes (n= 4 WT, 6 Cox6a2-/-). Two-tailed unpaired Mann-Whitney tests."
NCBI gene IDs:
"Cox6a2: 12862
Gad1: 14415
Gad2: 14417"
Figure legend: "(D) Representative images of PV+ interneurons from PV-CRE and PV-CRE;Cox6a2fl/fl mice showing PercevalHR fluorescence after excitation at 930 nm and 840 nm. White arrowheads indicate PercevalHR expressing cells. Scale bar: 20 μm."
NCBI gene IDs:
"PercevalHR: 
Cox6a2: 12862
CRE: 2777477
PV: 19293"
Figure legend: "(E) PV+ interneurons from PV-CRE;Cox6a2fl/fl mice exhibited significantly lower ATP-to-ADP ratios than PV+ interneurons from PV-CRE mice throughout the entire duration of the recordings (23 cells from 4 PV-CRE mice; 15 cells from 6 PV-CRE;Cox6a2-/- mice)."
NCBI gene IDs:
"Cox6a2: 12862
CRE: 2777477
PV: 19293"
Figure legend: "(A,B) The resting membrane potential and amplitude of action potentials in WT and Cox6a2-/- PV+ interneurons, respectively. Two-tailed unpaired Student's t-tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(C,D) The threshold for action potentials increased over maturation in WT but not in Cox6a2-/- PV+ interneurons. As a result, the threshold for action potentials was significantly lower in P34-45 Cox6a2-/- PV+ interneurons when compared to their WT counterparts. D: two-tailed unpaired Student's t-tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(E,F) Trains of action potentials were evoked by 2 sec depolarizing current pulses. The amplitude difference (difference between the mean amplitude of 1st-10th action potentials and 190th-200th action potentials) was significantly higher in P34-45 Cox6a2-/- compared to WT PV+ interneurons. In addition, the amplitude difference decreased with maturation in WT but not in Cox6a2-/- PV+ interneurons. F: two-tailed unpaired Mann-Whitney tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(G) While in WT PV+ interneurons the maximal firing frequency increased during maturation, such increase was abolished by Cox6a2 knockout. Thus, maximal firing frequency reached during 2 sec depolarizing current pulses was lower in P34-45 Cox6a2-/- compared to WT PV+ interneurons. Two-tailed unpaired Student's t-tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(I,J) Cox6a2-/- PV+ interneurons showed a negative correlation between age and dendritic length and between age and full branch depth (maximal number of branches between the soma and a terminal dendritic point), which contrasted with the expected positive correlation between these parameters in WT PV+ interneurons. Linear regression of Pearson correlation."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(K,L) WT and Cox6a2-/- PV+ interneurons showed similar values for dendritic length and full branch depth at P22-P33. However, at P34-P45, Cox6a2-/- PV+ interneurons showed lower dendritic length and full branch depth than WT PV+ interneurons. Two-way ANOVA followed by Tukey's multiple comparisons tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(M,N) While younger WT and Cox6a2-/- PV+ interneurons have similar dendritic tree complexities (M), at the later stage of maturation Cox6a2-/- PV+ interneurons show a reduction in dendritic tree complexity in comparison to WT PV+ interneurons (N). Multiple t-tests, two-stage step-up method of Benjamini, Krieger and Yekutieli."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(B) In the OFT, Cox6a2-/- mice travelled a longer distance and moved faster than WT mice. Cox6a2-/- mice also moved more often from the outer zone to the inner zone of the arena. Two-tailed unpaired Student's t-tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(D) In the SIT, Cox6a2-/- mice also travelled a longer distance with higher mean velocity than WT mice. Additionally, both Cox6a2-/- and WT mice spent more time exploring the container with the stranger mouse than the empty container. Total distance moved, mean velocity, and f [number of crossings] outer to inner zone: two-tailed unpaired Student's t-test. Social interaction: two-way ANOVA followed by Tukey's multiple comparisons test."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(F) The startle responses were significantly lower in Cox6a2-/- mice when the loud stimulus (120 dB) was presented alone the first 5 times. Two-way ANOVA followed by Tukey's multiple comparisons tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "(G) The PPI of the acoustic startle response was similar at all pre-pulses for Cox6a2-/- and WT mice. Two-way ANOVA followed by Sidak's multiple comparisons tests."
NCBI gene IDs:
"Cox6a2: 12862"
Figure legend: "B. Relative miR-34c-5p and miR-155-5p expression levels (2deltaCt normalized to RNU48) in unstimulated and stimulated naive CD4 T cells of individual samples pooled to generate the sequencing libraries. mean ± SEM and p-value for differences in miR-155-5p expression (paired two tailed t-test) are shown. Comparison could not be performed for miR-34c-5p as it is undetermined in unstimulated samples."
NCBI gene IDs:
"RNU48: 
miR-155-5p: 406947
miR-34c-5p: 407042"
Figure legend: "C. Time course quantification of miR-34c-5p expression (top panel, 2deltaCt values) in three different donors."
NCBI gene IDs:
"miR-34c-5p: 407042"
Figure legend: "A, B. Comparison of the mean miR expression level by in TCR-stimulated naive CD4 T cell small RNA-seq libraries from uninfected and HIV-1NL4-3 (A) or HIV-2ROD (B) infected samples (24h). Only miRNAs with a minimum of 10 normalized read counts. Library normalization was performed using the mean normalization method available in Deseq package of Bioconductor (Anders & Huber, 2010). Lines indicate changes of +/- 1 log2 fold between samples (n=3, paired pools of three individual samples per library). miR-34c-5p is highlighted in black."
NCBI gene IDs:
"miR-34c-5p: 407042"
Figure legend: "C. Quantification of miR-34c-5p expression levels of the individual samples pooled to generate the sequencing libraries by qPCR (mean±SEM 2deltaCt values and p value from a paired two tailed t test; n=5)."
NCBI gene IDs:
"miR-34c-5p: 407042"
Figure legend: "A. Cell-associated viral DNA levels (left), Tat mRNA levels (center), and RT activity levels (right) in cell culture supernatants in infected J34c cells vs. infected JØ cells at 24 and 48 hours post-infection. * p0.05 (paired two tailed t test)."
NCBI gene IDs:
"Tat: 155871"
Figure legend: "C. Fold change levels for miR-34c-5p, cell-associated viral DNA level, Tat mRNA, and RT activity levels in J34c cells treated with miR-34c-5p antagomiR versus control antagomiR, 48 hours post-infection. Differences to control were found to be significant with a paired two tailed t test (p0.05)."
NCBI gene IDs:
"miR-34c-5p: 407042
Tat: 155871"
Figure legend: "A. Hierarchical clustering of the 82 differentially expressed (DE) genes identified in Jurkat cell lines overexpressing miR-34c-5p (J34c-2 and J34c-5) vs control (JØ-1, 2 and 3 clones). Color scale represents mean centered log2 fold expression level across samples. Pie charts represent the relative proportion of predicted miR-34c-5p binding sites by the Miranda or PITA algorithms (or both) in down-regulated (top) and up-regulated (bottom) gene sets."
NCBI gene IDs:
"miR-34c-5p: 407042"
Figure legend: "B. qRT-PCR validation of selected genes. Plot represents the average percentage of target gene mRNA (normalized to GAPDH) in J34c vs JØ cells (all differences were found to be significant with a paired two tailed t test, P&lt;0.05; n=5)."
NCBI gene IDs:
"GAPDH: "
Figure legend: "B. Bottom: antagomiR to miR-34c-5p results in increased KAT2B mRNA levels compared to control-treated cells. Plot presents relative expression values for mRNA in 3 biological replicates; bars represent mean and SEM. Differences were found to be significant with p&lt;0.05 (paired two tailed t test)."
NCBI gene IDs:
"KAT2B: 8850
miR-34c-5p: 407042"
Figure legend: "C. Predicted target sites for miR-34c-5p in the PCAF/KAT2B mRNA(black bars) and miR-target hybridization for the two strongest sites (top); Luciferase assay with the pEZX renilla/firefly-PCAF/KAT2B 3'UTR reporter in HEK293 cells co-transfected with either the miR-34c-5p overexpression vector or pcDNA3 empty control (bottom). Differences were found to be significant with p&lt;0.05 (paired two tailed t test, n=5)."
NCBI gene IDs:
"KAT2B: 8850
PCAF: 8850
miR-34c-5p: 407042"
Figure legend: "a, Cells (wt, ∆atg18 or ∆atg18/∆atg21/∆hsv2 ()), with and without a plasmid expressing wt or a mutant form of Atg18) were grown in YPD, vacuoles were stained with FM4-64 and imaged by confocal microscopy. At least 10 confocal sections spaced at 300 nm were assembled into maximum projections. Images show cells before (0 min) and 15, 30 and 60 min after induction of vacuolar fragmentation by addition of 0.4 M NaCl. Red: FM4-64. Gray: DIC. Scale bar 5 µm."
NCBI gene IDs:
"atg18: 850577
Atg18: 850577
atg21: 856004
hsv2: 853138"
Figure legend: "atg18 cells expressing plasmids carrying Atg18-GFP or Atg18FGGG-GFP, a mutant in the lipid binding sites, were stained with FM4-64. a, Confocal z-stacks were taken before and 2, 6 and 10 min after addition of 0.4 M NaCl. Stacks were processed into maximum projections using ImageJ. Scale bar: 5 µm."
NCBI gene IDs:
"atg18: 850577"
Figure legend: "b, Same experiment as in a, but in a fab1 or vps34 background."
NCBI gene IDs:
"fab1: 850574
vps34: 850941"
Figure legend: "a. Autophagy measured with the pho8∆60 assay in atg18 cells complemented with wt, FGGG, SLoop or DLoop alleles of ATG18."
NCBI gene IDs:
"atg18: 850577"
Figure legend: "b. Same assay as in a, but in atg18 atg21 cells complemented with wt or SLoop versions of ATG18 and ATG21. Atg21SLoop contains K426F and F429K substitutions. Helical wheel projections for both Atg21 helices are given. The arrows indicate the magnitude of the hydrophobic moment."
NCBI gene IDs:
"atg18: 850577
atg21: 856004"
Figure legend: "c. Vacuole fragmentation in atg2 cells, assayed 15 min after a moderate hypertonic shock as in Fig. 1."
NCBI gene IDs:
"atg2: 855479"
Figure legend: "(a) Knockdown of RARα in NIH3T3 mouse fibroblasts was conducted using two different shRNAs, sh1 and sh2, compared to control (Ctr). Left, representative immunoblot. Actin is shown as loading control and full-length blots are shown in Supplementary Figure 21. Right, amounts of RARα in control and knockdown cells determined by densitometric quantification of immunoblots represented by the one shown on the left. Values are normalized for actin and expressed as multiples of control (None) values; n = 3."
NCBI gene IDs:
"RARα: 19401"
Figure legend: "(b) Rates of degradation of long-lived proteins in control and RARα(-) cells maintained in the presence or absence of serum for 12 h. Values are expressed as percentage of proteolysis; n = 3."
NCBI gene IDs:
"RARα: 19401"
Figure legend: "(a) Immunoblot for LC3-II in control mouse fibroblasts (Ctr) or those knocked down for RARα (RARα(−)) maintained in the presence or absence of serum for the indicated times. Where indicated, protease inhibitors (PI) against lysosomal proteolysis were added. Actin is shown as a loading control. 'I' and 'II' designate different forms of LC3, as described in the text. (b) Amounts of LC3-II determined by densitometric quantification of immunoblots. Values are expressed as fold change in value relative to serum-supplemented control cells; n = 4. (c) Ratio of the amounts of LC3-II in cells treated with protease inhibitors compared to untreated cells (None). Values are expressed as fold change in value relative to untreated cells; n = 4."
NCBI gene IDs:
"RARα: 19401"
Figure legend: "(f) Control and RARα(-) cells were transfected with the KFERQ-mCherry1 photoactivatable reporter, and after photoactivation they were maintained in medium with or without serum. Left, representative images. Graph shows quantification of the number of puncta per cell in &gt;50 cells using the CMA reporter in at least four different fields. Nuclei are labeled with DAPI. Scale bars, 10 μm. In b, c, e and f, all values are mean ± s.e.m. Differences with control (marked with asterisk) or with serum-supplemented cells (marked with §) are significant for P 0.05. Full-field fluorescence images and full-length blots are shown in Supplementary Figures 2 and 21, respectively."
NCBI gene IDs:
"RARα: 19401"
Figure legend: "(c) Mouse fibroblasts were cotransfected with the human RARα (hRARα) receptor, a relevant reporter luciferase plasmid and the non-retinoid-regulated Renilla reporter to control for transfection. Values show relative luciferase units (RLU) detected in cells subjected to the indicated concentrations of ATRA or the three retinoid derivatives for 12h ."
NCBI gene IDs:
"luciferase: 
Renilla: 
RARα: 5914"
Figure legend: "(d) Mouse fibroblasts were cotransfected with the human RARα (hRARα) receptor, a relevant reporter luciferase plasmid and the non-retinoid-regulated Renilla reporter to control for transfection.Cells transfected as in c were treated with 100 nM of ATRA alone or in the presence of the indicated concentrations of the three retinoid derivatives or the antagonist BMS614 for 12 h. Values are shown as RLU."
NCBI gene IDs:
"luciferase: 
Renilla: 
RARα: 5914"
Figure legend: "(e) Mouse fibroblasts were cotransfected with the human RXR receptor, a relevant reporter luciferase plasmid and the non-retinoid-regulated Renilla reporter to control for transfection. Values show relative luciferase units detected in cells subjected to the indicated concentrations of ATRA or the three retinoid derivatives for 12 h."
NCBI gene IDs:
"luciferase: 
Renilla: "
Figure legend: "(f) Mouse fibroblasts were cotransfected with the human RXR receptor, a relevant reporter luciferase plasmid and the non-retinoid-regulated Renilla reporter to control for transfection. Values show relative luciferase units detected in cells subjected to the indicated concentrations of ATRA or the three retinoid derivatives for 12 h. Cells transfected as in e were treated with 10 μM of ATRA alone or in the presence of the indicated concentrations of the three retinoid derivatives or the antagonist BMS614 for 12 h. Values are shown as RLU. Values in c-f are mean ± s.e.m.; n = 4-6."
NCBI gene IDs:
"luciferase: 
Renilla: "
Figure legend: "(a) Rates of degradation of long-lived proteins in control mouse fibroblasts or RARα(−) or LAMP-2A(−) (L-2A(−)) cells left untreated (None) or treated with 20 μM of the indicated compounds. Values are expressed as the fold change in proteolytic rate relative to the rate in untreated cells for each group; n = 3."
NCBI gene IDs:
"LAMP-2A: 16784
RARα: 19401"
Figure legend: "(b-d) Control mouse fibroblasts or RARα(−), LAMP-2A(−) or LAMP-2B(−) (L-2B(−)) cells were transfected with the KFERQ-mCherry1 photoactivatable reporter with or without the indicated compounds (20 μM). In b are shown the representative fields and high-magnification images (insets) for GR1. Nuclei are labeled with DAPI. Representative fields for GR2 and AR7 are shown in Supplementary Figure 13. Scale bars, 10 μm. (c,d) Average number of fluorescent puncta per cell quantified in &gt;50 cells in at least four different fields in control cells and RAR(−) (c) or LAMP-2B(−) (d) cells. No puncta were detected in LAMP-2A(−) cells."
NCBI gene IDs:
"LAMP-2A: 16784
LAMP-2B: 16784
RARα: 19401"
Figure legend: "(b) mRNA levels of LAMP-2A in control mouse fibroblasts (left) or RARα(−) cells (right) treated with AR7 or paraquat (PQ) as in a; n = 4-5. Values are presented as fold change in mRNA relative to untreated cells."
NCBI gene IDs:
"LAMP-2A: 16784
RARα: 19401"
Figure legend: "(c) Cellular viability of control (left) or LAMP-2A(−) (right) fibroblasts exposed to 2 mM or 0.5 mM paraquat, respectively, and treated with the indicated compounds for 12 h before or after the paraquat treatment; n = 3."
NCBI gene IDs:
"LAMP-2A: 16784"
Figure legend: "(d) Viability of mouse fibroblasts transfected with the indicated concentrations of a plasmid encoding α-synuclein and left untreated (None) or treated with 1 mM paraquat alone or in the presence of 20 μM AR7; n = 3. In b-d, all values are mean ± s.e.m. Differences with cells that are untreated (marked with asterisk) or those treated only with paraquat (§) were significant for P 0.001. Full-length blots are shown in Supplementary Figure 21."
NCBI gene IDs:
"α-synuclein: 20617"
Figure legend: "(e) Immunoblot for α-synuclein (α-syn) in the same cells as in d. Top, higher-exposure blot to highlight oligomeric (oligo) species. Asterisk denotes nonspecific band. M, monomer; MW, molecular weight."
NCBI gene IDs:
"α-synuclein: 20617"
Figure legend: "H. Internalization (cytoplasm) and recycling (plasma membrane) of FGF10-stimulated HA-FGFR2b (green) transfected in BT549 stimulated for 0, 40 and 120 min.. Cells were depleted or not of TTP or RCP by a pool of siRNAs. TRITC-Tf is a marker of recycling (red). Nuclei are stained in blue. *, cells with receptor recycled to the plasma membrane. Scale bar, 5 μm."
NCBI gene IDs:
"RCP: 80223
TTP: 23677"
Figure legend: "(J) control or 40 min. FGF10- or TGFα-stimulated T47D left untreated or depleted of TTP, followed or not by transfection with siRNA resistant Flag-TTP (Flag-TTP*) or depleted of RCP followed or not by transfection with siRNA resistant RCP-GFP (RCP-GFP*) were immunoblotted with the indicated antibodies. **, non-specific band (J)."
NCBI gene IDs:
"Flag: 
GFP: 
RCP: 80223
TTP: 23677"
Figure legend: "E. Co-localization of FGFR2 (red), EGFR (green), and the recycling marker Tf (blue) in T47D depleted of EGFR by siRNA followed by transfection with wt or T693A and stimulated or not with either FGF10 or TGFα for the indicated time periods. Scale bar, 5 μm."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "E, F Lysates from T47D (E) and BT20 (F) depleted of EGFR, transfected with wt or T693A, and stimulated or not with either FGF10 or TGFα for the indicated time intervals were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "A-D Percentage of EdU incorporation in T47D (A, C) or BT20 (B, D) depleted of EGFR or not, transfected with wt or T693A, and stimulated or not with FGF10 (A, B) or with TGFα (C, D) and pre-treated (C, D) or not (A, B) with FGF10 for 40 min. Values represent the median ± SD of N= 4. P value =&lt;0.05 *, &lt;0.01 **, &lt;0.001*** (one-way ANOVA with Tukey test)."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "(D) Immunofluorescence  of neurons  expressing BioID2-tau   or BioID2  (myc) or non-transduced control cells. Cells were incubated in biotin  medium (50 μM) or vehicle medium for 30 h prior to fixation and probed for biotin  (streptavidin, SA) and cell nuclei  (DAPI) (n = 3 biological replicates). BioID2-tau   and incorporated biotin co-localise in axons  and somata. Scale bar, 25 μm."
NCBI gene IDs:
"myc: 
tau: 4137"
Figure legend: "(B) Immunoblot  of combined brain  lysates from mice  (P35) expressing BioID2-tau   or BioID2  control (n = 4 biological replicates) probed for myc-tagged BioID2  and BioID2-tau. Controls: lysates from (1) BioID2- or (2) BioID2-tau-expressing and (3) non-transduced mouse brain. Loading, glyceraldehyde-3-phosphate dehydrogenase (Gapdh). *, unspecific band. Relative quantification confirmed similar expression levels of BioID2  and BioID2-tau. Means ± S.E.M. ns, not significant (Student's t-test)"
NCBI gene IDs:
"tau: 4137"
Figure legend: "(B-C)  Co-immunoprecipitation (Co-IP) of tau  and NSF   from mouse brain. (B) Endogenous and transgenic tau  were immunoprecipitated  from mouse brain  lysates of tau-/-, ALZ17tg or ALZ17non-tg mice  using anti-tau  antibody followed by immunoblot  probed for NSF, tau  and α-actin. Endogenous NSF  was co-immunoprecipitated  with transgenic and endogenous tau  from ALZ17tg or ALZ17non-tg but not from tau-/- with. GAPDH, input loading control (n = 3 biological replicates). Recombinant tau  and NSF  served as controls. (C) Densitometric  quantification of co-IP results in (B). Data information: Values are means ± S.E.M (normalized to control). Adjusted p-values: ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test."
NCBI gene IDs:
"tau: 17762
tau: 4137"
Figure legend: "Co-IP  of HA-tagged tau  with NSF  from 293T  cells. (E) Co-IP of NSF  with haemagglutinin  (HA)-tagged tau  variants from cultured cells. HA-tagged tau  variants and NSF  were expressed in 293T  cells followed by immunoprecipitation  using HA  antibody and immunoblot  probed for NSf, tau  and GAPDH. (n = 3 biological replicates)."
NCBI gene IDs:
"HA: 
tau: 4137
NSF: 4905"
Figure legend: "(A) Impaired AMPA-type glutamate receptor  surface expression  in cultured tau-/- neurons. Cortical tau+/+ or tau-/- neurons  were surface biotinylated after incubation with non-competitive NMDAR  antagonist MK-801  (50 μM, 3 min) and/or GABAAR antagonist bicuculline (30 μM, 10 min) at 37°C. Surface GluA1  levels were detected by immunoblot  after with streptavidin  (SA) enrichment. Input was probed for total cellular GluA1, NSF, tau   and  βIII-tubulin. (n = 3 biological replicates) Input and SA-enrichment from non-surface biotinylated neurons  served as negative controls. (B) Reduced neuronal  surface GluA1  in tau-/- neurons. Quantification of tau+/+ and tau-/- surface GluA1  upon treatment and SA-enrichment shown in (A). Data information: Values are means ± S.E.M (normalized to control). Adjusted p-values: ****p &lt; 0.0001, ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test. "
NCBI gene IDs:
"tau: 17762"
Figure legend: "(C) Cell surface GluA1  and total NSF immunostaining  in primary mouse hippocampal neurons (DIV15). Tau+/+, tau-/- neurons  and tau-/- expressing human tau  (tau-/-.AAVtau) were treated with either vehicle (Veh; DMSO) or MK-801 (50 μM) for 3 min. Representative confocal  images of whole cell (left) and neurites  (right) are shown (n = 3 biological replicates). NSF, surface GluA1  and cell nuclei  (DAPI) were visualized. Scale bar, 25 μm. (D-E) Quantification of neurite  surface GluA1  and neurite NSF  relative to tau+/+ Veh control. Reduced surface GluA1  (D) and total NSF  (n = 10-12 neurites  from 3 biological replicates) (E) localized  to neurites  in tau-/- neurons  treated with MK801  (n = 21-23 neurites  from 3 biological replicates). Data information: Values are means ± S.E.M (normalized to control). Adjusted p-values: ****p &lt; 0.0001, ***p &lt; 0.001, **p &lt; 0.01, *p &lt; 0.05, ns, not significant, ANOVA with Sidak's test. "
NCBI gene IDs:
"Tau: 17762
tau: 17762
tau: 4137"