EMBO/SourceData huggingface dataset:
Figure legend: "(D) Western blot analysis of EXO-CD24 exosomes. Tetracycline (Tet) was used to induce CD24 expression in the cell line from which exosomes are derived. HSP70 was used as an exosomal marker."
NCBI gene IDs:
"CD24: 100133941"
Figure legend: "Y-axis, z-score; X-axis, timepoints. A indicates asynchronous  cells, M mitotic  cells and the numbers represent the minutes after release from the mitotic  block). Dots show individual replicates and lines averages (n=3 for A and M; n=2 for each post-mitotic   sample). Pre-mRNA levels are shown for pluripotency genes (Oct4, Klf4, Esrrb) and cell cycle regulators (E2f1, Cdk2, Rb); mRNA levels for individual histone   genes (only averages are shown)."
NCBI gene IDs:
"Cdk2: 12566
E2f1: 13555
Esrrb: 26380
Klf4: 16600
Oct4: 18999
Rb: 19645"
Figure legend: "(B) Heatmaps presented for untreated (-IAA; left) or CTCF-depleted (+IAA; right) CTCF-AID cells."
NCBI gene IDs:
"CTCF: 13018"
Figure legend: "(A) Gene expression changes (z-score) in asynchronous  cells and during  post-mitotic  release of CTCF-AID cells treated or not with IAA  to deplete CTCF  (blue, presence of CTCF; red, absence). Samples are indicated on the X-axis (A: asynchronous  cells; M: mitotic  cells; numbers: minutes since release from mitotic  block). Six gene groups are shown, based on the time  of their response to CTCF  depletion (asy, late, early) and the direction of the transcriptional change (up, down). The number of genes of each group is indicated. The yellow shadow areas indicate times  showing expression  changes. The boxplots show the median (bar), 25-75% percentiles (box) and 1.5-fold inter-quartile range (whiskers)."
NCBI gene IDs:
"CTCF: 13018"
Figure legend: "(C) Average binding  profile (X-axis, reads per million) of CTCF  (top) and the Cohesin subunit SMC1   (bottom), across 8kb centred on the TSS of genes responding to CTCF  depletion exclusively in asynchronous  cells (left) or either late or early upon mitotic  release (middle and right, respectively). Interphase  is shown in blue and mitosis  in red."
NCBI gene IDs:
"CTCF: 13018"
Figure legend: "Presence of TOC proteins in the  central vacuole of protoplasts. Arabidopsis protoplasts  expressing the YFP-tagged chloroplast  proteins were analyzed by confocal microscopy following ConA  treatment for four hours. Representative images are shown (A). Chlorophyll   autofluorescence was used to determine the localization  of the YFP  fluorescence signals relative to the chloroplasts. Signals for SP1-YFP  and FAX1-YFP, which here are control proteins, were only present at the chloroplast envelope. Accumulation of YFP  puncta in the  central vacuole  is indicated by the arrowheads. Scale bar, 10 μm."
NCBI gene IDs:
"YFP: "
Figure legend: "Presence of TOC proteins in the  central vacuole of Arabidopsis leaf  and hypocotyl  cells. Eight-day-old transgenic Arabidopsis  seedlings stably expressing pToc33:YFP-Toc33  or pToc75:YFP-Toc75  were treated with ConA  for two days, and then analyzed by confocal microscopy. Accumulation of YFP  puncta in the central vacuole is indicated by the arrowheads. Scale bar, 10 μm."
NCBI gene IDs:
"YFP: 
Toc33: 839248
Toc75: 823827"
Figure legend: "Lack of TOC proteins in the  central vacuole  of atg7-2 mutant hypocotyl  cells. Eight-day-old transgenic Arabidopsis  seedlings stably expressing pToc33:YFP-Toc33  and pToc75:YFP-Toc75  in the atg7-2 mutant background were treated with ConA  for two days, and then analyzed by confocal microscopy. Note that YFP-Toc33  and YFP-Toc75  bodies (arrowheads) were retained at  plastid  bridges and extensions, and not released into the vacuole, as occurs in the WT background Scale bar, 10 μm."
NCBI gene IDs:
"YFP: 
atg7: 834630
Toc33: 839248
Toc75: 823827"
Figure legend: "Autophagy inhibition affected accumulation of TOC proteins. Eight-day-old wild-type (WT) and atg7-2 plants were treated respectively with proteasome  inhibitor, bortezomib  (Btz), cysteine protease  inhibitor, E64d, or DMSO  (Mock) for one day. Total protein extracts from these plants were analyzed by immunoblotting  using antibodies as indicated. Actin  acted as a loading control. Typical immunoblotting  results are shown (B). In B, the arrowhead indicates full-length Toc159, and the hollow arrow indicates a truncated form of Toc159. Molecular weight markers (sizes in kD) are indicated to the right of the images."
NCBI gene IDs:
"atg7: 834630"
Figure legend: "Autophagy inhibition affected accumulation of TOC proteins. Eight-day-old wild-type (WT) and atg7-2 plants were treated respectively with proteasome  inhibitor, bortezomib  (Btz), cysteine protease  inhibitor, E64d, or DMSO  (Mock) for one day. Band intensities were quantified and normalized to equivalent data for Actin  (C). Data are means ± SEM from three biological replicates. Asterisks in C indicate significance according to an unpaired two-tailed Student's t-test (*, significant at P &lt; 0.05; **, significant at P &lt; 0.01)."
NCBI gene IDs:
"atg7: 834630"
Figure legend: "Presence of autophagosome  in the chloroplast envelope. Arabidopsis protoplasts  expressing free YFP  and YFP-ATG8  (autophagosome  marker) were analyzed by confocal microscopy. Corresponding chlorophyll  autofluorescence images were employed to orientate the YFP  signal in relation to the chloroplasts. For eliminating interference from cytosolic  signals, intact protoplasts  (upper panels) were ruptured using 10-μm nylon mesh to isolate the chloroplasts. Retention of YFP  signals in association with chloroplasts   upon rupturing the cell (lower panels) clearly indicated that the YFP-ATG8  was associated with chloroplasts. Representative images are shown (A)."
NCBI gene IDs:
"YFP: 
ATG8: 828287"
Figure legend: "Presence of NBR1-HA  in chloroplast  fraction. The protoplasts  were isolated from four-week-old transgenic Arabidopsis plants stably expressing p35S:NBR1-HA  (NBR1-HA) and p35S:YFP-HA  (YFP-HA), followed by chloroplast  purification, respectively. Protein samples of whole protoplasts  and isolated chloroplasts  of NBR1-HA  and YFP-HA  were then analyzed by SDS-PAGE and immunoblotting, using antibodies against the HA  tag and Tic40. Typical immunoblotting  results are shown (G)."
NCBI gene IDs:
"HA: 
YFP: 
NBR1: 828571"
Figure legend: "BiFC  results showed interaction between NBR1  and TOC proteins. cYFP and nYFP  refer to C-terminal YFP  fragment and N-terminal YFP  fragment, respectively. Reconstitution of YFP  fluorescence was assessed after transient coexpression of the indicated pairs of fusion proteins. CDKA1  and OEP7  were used as negative controls for OEM  and cytosolic proteins, respectively. Representative images are shown. Scale bars, 10 μm.­ The frequency of protoplasts  showing a BiFC  signal in C was quantified. All data are means ± SEM from three experiments."
NCBI gene IDs:
"cYFP: 
nYFP: 
YFP: "
Figure legend: "NBR1 coimmunoprecipitated  with TOC proteins. Proteins were extracted from eight­-day-old transgenic Arabidopsis  seedlings stably expressing p35S:NBR1-HA  or p35S: NBR1-ΔUBA-HA, or control seedlings only expressing p35S:YFP-HA, following UVB  treatment for four hours. Obtained protein extract was analyzed by IP  using anti-HA  resin, and analyzed by immunoblotting  of four endogenous proteins or HA-tagged proteins. Tic40  acted as negative controls. The asterisks indicate nonspecific cross-reacting bands. Arrow indicates the NBR1-HA  band. The arrowhead indicates full-length Toc159  protein. Molecular weight markers (sizes in kD) are indicated to the right of the images."
NCBI gene IDs:
"HA: 
YFP: 
NBR1: 828571"
Figure legend: "Phenotypes of seedlings  under UVB  stress. Briefly, eight-day-old WT, atg7-2, nbr1-2 and NBR1-OX (overexpressor) seedlings  grown under normal condition were subjected to UVB  stress, with UVB  treatment for 3 hours and recovery  for 7 days. Representative plant images (A) and total chlorophyll  content values (B) are shown; the latter provide a convenient metric for biomass. The chlorophyll  values for the mutants and overexpressor plants were statistically significantly different from that for the WT (Student's t test; *, significant at P &lt; 0.05; ***, significant at P &lt; 0.001)."
NCBI gene IDs:
"atg7: 834630
nbr1: 828571
NBR1: 828571"
Figure legend: "TOC protein levels were regulated by autophagy in response to short-term UVB   stress. Eight-day-old plants of indicated genotypes were subjected to UVB  stress for the indicated times, and then analyzed by immunoblotting  using 10 μg total protein extract loaded per lane (C, upper). Coomassie staining indicated that the treatments did not change the overall protein profile (C, lower). Band intensities were quantified and normalized to equivalent Actin  data (D) Time  0 was taken as 100%. Unpaired two-tailed Student's t-tests revealed that the Toc159  protein level in nbr1-2 (P &lt; 0.05) and NBR1-OX (P &lt; 0.05), the Toc75  protein level in atg7-2 (P &lt; 0.05), nbr1-2 (P &lt; 0.01), and NBR1-OX (P &lt; 0.05), and the Toc33  protein level in atg7-2 (P &lt; 0.05), nbr1-2 (P &lt; 0.01), and NBR1-OX (P &lt; 0.05), were significantly different from levels in the WT at the time  of 1 d recovery   from UVB  stress."
NCBI gene IDs:
"atg7: 834630
nbr1: 828571
NBR1: 828571"
Figure legend: "TOC protein levels were regulated by autophagy in response to short-term heat  stress. Eight-day-old plants of indicated genotypes grown in vitro were subjected to short term heat  stress, recovered for the indicated times, and then analyzed by immunoblotting  using 10 μg total protein extract loaded per lane (B, upper). Coomassie staining indicated that the treatments did not change the overall protein profile (B, lower). Band intensities were quantified and normalized to equivalent Actin  data (C). Time  0 was taken as 100%. Data are means ± SEM from three biological replicates. Unpaired two-tailed Student's t-tests revealed that the Toc159  protein level in nbr1-2 (P &lt; 0.05) and NBR1-OX (P &lt; 0.01), and the Toc75  protein level in NBR1-OX (P &lt; 0.05), were significantly different from levels in the WT at Time  12 h; and that the Toc159  protein level in atg7-2 (P &lt; 0.001), nbr1-2 (P &lt; 0.01), and NBR1-OX (P &lt; 0.001), and the Toc75  protein level in atg7-2 (P &lt; 0.01), nbr1-2 (P &lt; 0.01), and NBR1-OX (P &lt; 0.01), were significantly different from levels in the WT at Time  24 h."
NCBI gene IDs:
"atg7: 834630
nbr1: 828571
NBR1: 828571"
Figure legend: "Analysis of  protein import efficiency  in vitro. Chloroplasts  were isolated from 12-day-old WT, nbr1-2 and NBR1-OX seedlings  grown in vitro under normal condition, and were subjected to in vitro protein import assay by using 35S-labeled Rubisco small subunit  (SSU) precursor protein as the import substrate, followed by corresponding quantification of the maturation of the radiolabeled    precursor protein. A representative phosphor  screen image is shown (upper left), and Coomassie staining  of Rubisco large subunit (LSU) indicated equal loading (lower left); Times  indicate minutes after the start of each import reaction. IVT, in vitro translation product used as a control. Together with similar images from two additional experiments, this was used to conduct the quantitative analysis shown (right); the amount of imported, mature protein in each sample was expressed as a percentage of that present at the final  time point for the WT. Data are means ± SEM from three biological replicates."
NCBI gene IDs:
"nbr1: 828571
NBR1: 828571"
Figure legend: "Chloroplast  development in WT, nbr1-2 and NBR1-OX seedlings  under UVB  stress was studied. Cotyledons  of eight-day-old plants grown in vitro under normal condition (D) or UVB  stress (E) were analyzed by transmission electron microscopy, and representative images are shown. UVB  stress was conducted in the same procedures as in C. In D and E, the upper images are at the same magnification (scale bar, 2 μm); and below these are high magnification (5×) images corresponding to the boxed regions above. These and similar micrographs of chloroplasts were used to determine chloroplast  shape (F), the number of   thylakoid lamellae per granum  (G), and the number of starch granules  per chloroplast  (H) in each genotype, with or without UVB  stress. Asterisks indicate significance according to an unpaired two-tailed Student's t-test (*, significant at P &lt; 0.05; ***, significant at P &lt; 0.001; ****, significant at P &lt; 0.0001). Values are means ± SEM from 30-50 chloroplasts."
NCBI gene IDs:
"nbr1: 828571
NBR1: 828571"
Figure legend: "Phenotypes of two-week-old WT, nbr1-2, ppi1  and ppi1 nbr1-2 seedlings  grown on soil. Representative plant images (A) and chlorophyll  contents (B) are shown. The chlorophyll  values for the ppi1  and ppi1 nbr1-2 plants were statistically significantly different (Student's t test; ****, significant at P &lt; 0.0001). Values are means ± SEM from 20 plants."
NCBI gene IDs:
"nbr1: 828571
ppi1: 828859"
Figure legend: "Chloroplast  development in ppi1  and ppi1 nbr1-2 seedlings  was studied. Cotyledons  of two-week-old plants grown under normal condition as in A were analyzed by transmission electron microscopy, and representative images are shown (E). In E, the upper images are at the same magnification (scale bar, 1 μm); and below these are high magnification (3×) images corresponding to the boxed regions above. These and similar micrographs of chloroplasts  were used to determine chloroplast  size (F) and the number of thylakoid lamellae per granum  (G) in each genotype. Asterisks indicate significance according to an unpaired two-tailed Student's t-test (****, significant at P &lt; 0.0001). Values are means ± SEM from 30-40 chloroplasts."
NCBI gene IDs:
"nbr1: 828571
ppi1: 828859"
Figure legend: "(a) Plasma ornithine  concentrations in Oatrhg mice  following intravenous injections of AAV-OAT  at the doses of 1x1013 (n=9) and 3x1013 genome copies (gc)/Kg (n=6), or AAV-GFP  at the dose of 1x1013 gc/Kg (n=9). Normal range was established on wild-type mice  (n=7) and average ± 2SD was calculated. Data are shown as averages ± SEM. ***p&lt;0.001, ****p&lt;0.0001 (Two-way ANOVA test)."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(c) Plasma ornithine  and lysine  concentrations at 12 months of age (10-months post-injection) in  Oatrhg mice injected with AAV-OAT  (n=3) or AAV-GFP  (n=3); WT mouse  controls (n=5) are also shown; each sample was analyzed as triplicates, data are shown as averages ± SEM. One-way ANOVA test with Tukey correction; *p &lt;0.05; ****p&lt;0.0001."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(e) Western blot for OAT  and GFP  in livers  of Oatrhg mice  injected with AAV-GFP  or AAV-OAT. A WT mouse liver  was included as control. Vinculin  was used as loading control."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(g) Liver immunohistochemistry  showing approximately 10% of OAT-positive cells in  Oatrhg mice  injected with AAV-OAT. Livers  of WT and Oatrhg mice  injected with AAV-GFP  are shown as controls. 5x (images on the left side) and 20x (images on the right side) fields of view are shown."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(h) Representative H&amp;E staining  of the retinas  of 11-month-old Oatrhg mice  injected with AAV-GFP  or AVV-OAT  at the doses of 1x1013 or 3x1013 gc/Kg; age-matched WT mice  and uninjected Oatrhg mice  were included as controls (40X). Abbreviations: OS, outer segment; IS, inner segment; RPE,  retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(a) Representative electron micrographs of the mouse retina  of an AAV-GFP  injected Oatrhg mouse  showing retinal pigmented epithelium  (RPE) and adjacent abnormal  outer segment  (OS) disk membranes (black arrows). Phagolysosomes  containing undigested OS disc membranes are present (asterisks). (b) Retina  of AAV-OAT  injected Oatrhg mice  showing  photoreceptor disk membranes  (white arrows) and RPE  ultrastructure comparable to age matched WT mice  (c, white arrows). (c) Control retina  of WT mouse  is shown as comparison."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "(a) Plasma ornithine  concentrations in AAV-GFP- (n=3) and AAV-OAT-injected (n=3) OatΔ mice. Normal range of wild-type mice  is also shown. Each value plotted represents mean ± SEM; Two-way ANOVA test, *p &lt;0.05, **p&lt;0.01."
NCBI gene IDs:
"GFP: 
OAT: 4942
Oat: 18242"
Figure legend: "(c) Retinal  H&amp;E (40X) of  OatΔ mice  injected with either AAV-GFP  or AAV-OAT. Representative of three mice  per groups are shown; WT mice  were included as control. Abbreviations: OS, outer segment; IS,  inner segment; RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer."
NCBI gene IDs:
"GFP: 
Oat: 18242
OAT: 4942"
Figure legend: "mRNA expression levels of the indicated genes were assayed by real-time PCR and normalized to Actb (C)."
NCBI gene IDs:
"Actb: "
Figure legend: "Flag-PGC-1α was introduced into PGC-1α knockdown brown preadipocytes for 24 h and samples were collected for western blotting analyses of the indicated proteins (D). Expression levels of the indicated proteins were quantified (E) (mean ± SEM; n=3). Scramble, scramble shRNA."
NCBI gene IDs:
"Flag: 
PGC-1α: 19017"
Figure legend: "Samples were collected for real-time PCR analysis. The expression of Fundc1 was normalized (F)."
NCBI gene IDs:
"Fundc1: 72018"
Figure legend: "(G-H) Scramble and PGC-1α knockdown preadipocytes were induced to differentiate into mature adipocytes and samples were collected for western blotting analyses (G) and quantification of the indicated proteins (H)"
NCBI gene IDs:
"PGC-1α: 19017"
Figure legend: "(B) Luciferase activity stimulated by the human Fundc1 promoter. The activity of Fundc1 promoter fragments, either wild type (WT) or with a mutation in the potential NRF1 binding site, was assayed using a dual luciferase reporter system."
NCBI gene IDs:
"Luciferase: 
Fundc1: 139341"
Figure legend: "(C,D) EMSA assay using biotinylated oligonucleotide probes containing the predicted NRF1 binding site or mutant NRF1 binding site in the promoter of Fundc1 (F1 probe or mut F1 probe) ,the confirmed NRF1 binding site in the promoter of VSNL1 (positive probe). The probes were incubated with HeLa nuclear extracts, then run on a gel (C). A super-shifted band (asterisk) is detected in the presence of an anti-NRF1 antibody (D). Labeled probe: biotin labeled wild type Fundc1 probe; Cold probe: wild type Fundc1 probe; Mut probe: NRF1 binding site mutant Fundc1 probe; asterisk indicates the supershifted band generated with antibody to NRF1."
NCBI gene IDs:
"Fundc1: 139341
VSNL1: 139341"
Figure legend: "(E,F) ChIP assay to analyze the interaction of NRF1 (E) or PGC-1α (F) proteins with the Fundc1 promoter."
NCBI gene IDs:
"Fundc1: 139341"
Figure legend: "(G) NRF1-myc was introduced into NRF1 shRNA knockdown cells for 24 h and scramble cells were used as control and samples were collected for western blotting with the indicated antibodies. (H) Scramble and NRF1 shRNA knockdown cells were transfected with vector and PGC-1α-Flag for 24 h and samples were collected for western blotting with the indicated antibodies. "
NCBI gene IDs:
"Flag: 
myc: 
NRF1: 4899
PGC-1α: 10891"
Figure legend: "(I) ChIP analysis of the interaction of PGC-1α protein with the Fundc1 promoter in scramble or NRF1 shRNA knockdown cells."
NCBI gene IDs:
"Fundc1: 139341
NRF1: 4899"
Figure legend: "(A,B) BATs isolated from Fundc1fl/fl/Ucp1cre- (Cre-) and Fundc1fl/fl/Ucp1cre+ (Cre+) mice at 30℃ or following 72 h at 4℃ were analyzed by transmission electron microscopy. Representative images are shown, scale bar, 1μm (A). The number of mitophagosomes per 100 mitochondria were counted and normalized (B)."
NCBI gene IDs:
"cre: 2777477
Cre: 2777477
Fundc1: 72018
Ucp1: 22227"
Figure legend: "(C,D).Representative fluorescence images of BAT sections from Fundc1fl/fl/Adiponectincre- (Cre-) and Fundc1fl/fl/Adiponectincre+ (Cre+) mito-Keima mice at normal conditions (30℃) or 4℃ for 72 h. Scale bar, 50 μm (C). Quantification of the ratio between red and green signals (D)."
NCBI gene IDs:
"Adiponectin: 11450
cre: 2777477
Cre: 2777477
Fundc1: 72018"
Figure legend: "(E) Fundcfl/fl/Ucp1cre- (Cre-) and Fundcfl/fl/Ucpcre+ (Cre+) mice were maintained at 30℃ (Normal) or exposed to 4℃ for 72 h (Cold). Mitochondrial DNA copy numbers were analyzed by null and normalized to nuclear DNA standards."
NCBI gene IDs:
"cre: 2777477
Cre: 2777477
Fundc: 72018
Ucp: 22227
Ucp1: 22227"
Figure legend: "(F) The relative total mito-keima signal (green signal ) in Fundc1 deficiency and control mito-keima mice under normal condition or cold exposure were quantified and plotted."
NCBI gene IDs:
"Fundc1: 72018"
Figure legend: "(G) BATs isolated from Fundc1fl/fl/Ucp1cre- (Cre-) and Fundc1fl/fl/Ucp1cre+ (Cre+) at 30℃ or following 72 h at 4℃ were analyzed by transmission electron microscopy. The percent of mitochondria without cristae is shown in the histogram."
NCBI gene IDs:
"cre: 2777477
Cre: 2777477
Fundc1: 72018
Ucp1: 22227"
Figure legend: "Oxygen consumption rate (OCR) (H) were measured in BAT from Fundc1fl/fl/Ucp1cre- (Cre-) and Fundc1fl/fl/Ucpcre+ (Cre+) mice kept at 30℃ or at 4℃ for 72 h."
NCBI gene IDs:
"Cre: 2777477
cre: 2777477
Fundc1: 72018
Ucp: 22227
Ucp1: 22227"
Figure legend: "mitochondrial superoxide (I) were measured in BAT from Fundc1fl/fl/Ucp1cre- (Cre-) and Fundc1fl/fl/Ucpcre+ (Cre+) mice kept at 30℃ or at 4℃ for 72 h."
NCBI gene IDs:
"cre: 2777477
Cre: 2777477
Fundc1: 72018
Ucp: 22227
Ucp1: 22227"
Figure legend: "(A) Body temperature measurements in Fundc1fl/fl/Ucp1cre- (Cre-) and Fundc1fl/fl/Ucp1cre+ (Cre+) fasting male mice before and during cold exposure (4°C) for the indicated time. Body temperature (B) before and after 72 h cold exposure (4°C) of Cre- and Cre+ male mice."
NCBI gene IDs:
"cre: 2777477
Cre: 2777477
Fundc1: 72018
Ucp1: 22227"
Figure legend: "BAT tissue weight (C) measurements before and after 72 h cold exposure (4°C) of Cre- and Cre+ male mice."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Fundc1fl/fl/Ucpcre- and Fundc1fl/fl/Ucp1cre+ mice were exposed to 4℃ for 72 h (Cold), and control mice were kept at 30℃ (Normal). Western blotting analysis of BAT protein levels (D). Protein expression levels were normalized to Actin (E)."
NCBI gene IDs:
"cre: 2777477
Fundc1: 72018
Ucp: 22227
Ucp1: 22227"
Figure legend: "Fundc1fl/fl/Ucpcre- and Fundc1fl/fl/Ucp1cre+ mice were exposed to 4℃ for 72 h (Cold), and control mice were kept at 30℃ (Normal). Levels of mRNAs encoding Fundc1 and Ucp1 were measured by real-time PCR and normalized to Actb (F)."
NCBI gene IDs:
"Actb: 
cre: 2777477
Fundc1: 72018
Ucp: 22227
Ucp1: 22227"
Figure legend: "(G) Representative images of UCP1 immunohistochemistry of BAT sections from Cre- or Cre+ mice in normal conditions (30℃) or following 72 h at 4℃. Scale bar, 50 μm."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Sanger sequencing of the germline NF1 mutation c.350T&gt;A present in the patient that developed two different tumors (MPNST-NF1-001 and MPNST-NF1-002). Sequencing results from normal tissue, primary tumor (PT), and orthotopic xenograft tumors (OT) are shown. The sequence of the NF1 region revealed WT NF1 alleles in primary tumor samples, and not in the corresponding derived orthoxenografts, indicating probable loss of the human stromal cells."
NCBI gene IDs:
"NF1: 4763"
Figure legend: "SNP array of primary tumor (PT) and orthotopic xenograft passages 1 (OT P1) and 4 (OT P4) for MPNST-NF1-001 and MPNST-NF1-002 tumors. Results correspond to chromosome 17. Images show the B-allele frequency (BAF) for the different samples as scatter plots and the copy number callings below them represented by thick horizontal lines: 2n regions are shown in gray, gained regions in orange, lost regions in green, and LOH regions are represented in blue. The vertical red line indicates the location of the NF1 locus. In addition, the percentage of tumor versus stromal cells for each sample is represented in a pie chart (blue, stromal cells; red, tumor cells)."
NCBI gene IDs:
"NF1: 4763"