EMBO/SourceData huggingface dataset:
Figure legend: "B. Histone mark levels were examined at selected genes repressed in Rev3l-/- MEFs (Hoxb2, Hoxb8, WT1). Histograms represent enrichment of H3K9me3 and  H3K27me3 at indicated loci assessed by  ChIP-qPCR  in Rev3l+/+ and Rev3l-/- MEFs. Error bars indicate standard error of the mean for three independent experiments."
NCBI gene IDs:
"Hoxb2: 103889
Hoxb8: 15416
Rev3l: 19714
WT1: 22431"
Figure legend: "C. H3K9me3 and H3K27me3 mark levels were evaluated at selected genes that localized in genomic loci displaying replication timing delay and downregulated in  Rev3l-/- MEFs (FoxG1, GATA6). Histograms represent enrichment of H3K9me3 and H3K27me3 assessed by ChIP-qPCR at indicated loci in Rev3l+/+ and Rev3l-/- MEFs. Error bars indicate standard error of the mean for three independent experiments."
NCBI gene IDs:
"FoxG1: 15228
GATA6: 14465
Rev3l: 19714"
Figure legend: "G. MEFs cells were transfected with GFP-REV3L761-1029 WT PSVVL or ASAVA mutant and fixed with 4% formaldehyde. The distribution of GFP-REV3L constructs was detected by autofluorescence (green), chromocenters  were visualized by HP1α immunostaining (red) and DNA was  counterstained with DAPI. Scale bar= 10 μm"
NCBI gene IDs:
"GFP: 
REV3L: 5980"
Figure legend: "H. Human 293T cells were transfected with either FH-REV3L700-900, mutant V802D F-H-REV3L700- 900 or empty vector. Forty-eight hours after transfection cell lysates were made and used for immunoprecipitation with HP1 antibody. Western blot  was processed and membranes were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"REV3L: 5980"
Figure legend: "I. 293T  cells were co-transfected with F-H-REV3L700-900 or empty vector and V5-HP1γ, mutant IY/EE V5-HP1γ  or empty vector. Forty-eight hours after transfection cell lysates were made and used for immunoprecipitation  with α-V5 antibody. After  electrophoresis, samples were  immunoblotted  with anti-HA, anti-HP1γ or anti-α-Tubulin  as indicated."
NCBI gene IDs:
"V5: 
HP1γ: 11335
REV3L: 5980"
Figure legend: "B. Asynchronous Rev3l+/+ and Rev3l-/- MEFs  were fixed with PFA then subjected to in situ proximity ligation assay  (PLA) using 53BP1  and HP1α antibodies then PLA foci were counted in both cell lines (more than 150 nuclei  for each condition were counted). p-values were calculated by Mann-Whitney test ( ****: p&lt;0.0001). Red lines indicate the mean values. Error bars: SEM. Controls with a single antibody are also shown. Experiments were repeated three times. Representative images are shown. Scale bar= 5 μm."
NCBI gene IDs:
"Rev3l: 19714"
Figure legend: "C. Asynchronous Rev3l+/+ and Rev3l-/- MEFs were subjected to PLA  as by using γH2AX  and HP1β antibodies and processed as in (B). p-values were calculated by Mann-Whitney test ( *: p&lt;0.05). Red lines indicate the mean values. Error bars: SEM. Controls with a single antibody are also shown. Three independent experiments were performed."
NCBI gene IDs:
"Rev3l: 19714"
Figure legend: "Rev3l+/+ and Rev3l-/- MEFs were incubated with or without 0.23 μM aphidicolin for 24h before metaphase spreading. FISH  was performed using major satellite probe to quantify breaks in pericentromeric regions (F). Representative images of chromosomes showing abnormalities (see arrows) in pericentromeric regions from Rev3l-/- MEFs. Chromosomes were labelled with  DAPI  Error bars indicate standard error of the mean for three independent experiments. Mann-Whitney test (ns: not significant, * p&lt;0.05; *** p&lt; 0.001)."
NCBI gene IDs:
"Rev3l: 19714"
Figure legend: "A. Asynchronous Rev3l+/+ and Rev3l-/- MEFs were UV-irradiated  at the indicated doses and harvested 6 h or 24 h later. Cell lysates were analyzed by immunoblotting with indicated antibodies. B. Asynchronous Rev3l-/- MEFs  complemented with empty vector (EV) or REV3L were UV-irradiated  at the indicated doses and harvested 6 h or 24 h later. Cell lysates were analyzed by immunoblotting  with indicated antibodies. C "
NCBI gene IDs:
"Rev3l: 19714
REV3L: 19714"
Figure legend: "Asynchronous Rev3l+/+ and Rev3l-/- MEFs were pulse labelled for 15 min using 10 μM  EdU then UV-irradiated  at 4 J/m2. Cells pre-treated with CSK buffer were fixed and 53BP1, RPA  and RAD51 were detected by immunofluorescence. Representative images in non-irradiated  cells (NI) and quantification of 53BP1  foci in  EdU positive cells at different time points  are shown Scale bar= 10 μm The  number  of cells analyzed is indicated in (n) on each graph. ***p &lt; 0.001 (Kruskal-Wallis test). The presented data are representative of 2 repeats). Bars represent the median ± interquartile range"
NCBI gene IDs:
"Rev3l: 19714"
Figure legend: "F-H. Asynchronous Rev3l-/- MEFs complemented with empty vector (EV) or  REV3L  were processed as in (C). Quantification of 53BP1 foci in  EdU positive cells at different time points is shown (F). Quantification of the intensity of chromatin-bound RPA2 (G) and  RAD51  foci (H) 24 hours after UV-irradiation  was performed. The number of cells analyzed is indicated in (n) on each graph. ***p &lt; 0.001 (Kruskal-Wallis test). The presented data are representative of 2 repeats. Bars represent the median ± interquartile range."
NCBI gene IDs:
"Rev3l: 19714
REV3L: 19714"
Figure legend: "The violin plot of ACE2 gene expression of all major cell types."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "Heatmap of marker genes of 16 cell types identified. The red box highlights the cell type specific expression of ACE2."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "The violin plot of ACE2 gene expression across all cell types."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "Volcano plot highlighting proteins significantly enriched (open circles, FDR&lt;0.05) in eNOS-FLAG immunoprecipitates from growth factor-stimulated human endothelial cells; n=3 independent cell batches."
NCBI gene IDs:
"FLAG: 
eNOS: 4846"
Figure legend: "Nitrite in the cell supernatant of HEK293 cells expressing wild-type (WT), Y657F (YF) or Y657D (YD) eNOS and treated with solvent (Sol) or ionomycin (Io, 1 µmol/L, 15 min); n=6 independent experiments (2-way ANOVA and Bonferroni)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "The FMN/FAD ratio measured in eNOS immunoprecipitates from cells expressing WT, YF or YD eNOS; n=6 independent experiments (1-way ANOVA and Newman-Keuls)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "Pyruvate kinase (PK) activity in FLAG immunoprecipitates from human endothelial cells expressing FLAG-tagged wild-type (WT), Y657F (YF) or Y657D (YD) eNOS; n=6 independent cell batches (1-way ANOVA and Tukey)."
NCBI gene IDs:
"FLAG: 
eNOS: 4846"
Figure legend: "PK activity in eNOS immunoprecipitates from HEK293 cells expressing WT, YF, YD or S633D (SD) eNOS; n=5-9 independent experiments (1-way ANOVA and Bonferroni)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "S-nitrosation of PKM2 in human endothelial cells expressing FLAG-tagged WT or YF eNOS and treated with H2O2 (30 µmol/L) for 15 min; n=8 independent cell batches (Unpaired Student's t test). Dithiothreitol (DTT) was included to demonstrate specificity of the S-NO signal."
NCBI gene IDs:
"FLAG: 
eNOS: 4846"
Figure legend: "PKM2 S-nitrosation in HEK293 cells expressing YF or YD eNOS and either FLAG-tagged WT PKM2 or a C358S (CS) PKM2 mutant. SNO-FLAG-PKM2 was detected with an anti-FLAG antibody after biotin switch technique; n=4 independent experiments (2-way ANOVA &amp; Bonferroni). DTT treatment was included to demonstrate specificity of the SNO signal."
NCBI gene IDs:
"FLAG: 
eNOS: 4846
PKM2: 5315"
Figure legend: "PK activity in eNOS immunoprecipitates from HEK293 cells co-expressing YF or YD eNOS and either WT PKM2 or the CS PKM2 mutant; n=6 independent experiments (2-way ANOVA &amp; Bonferroni)."
NCBI gene IDs:
"eNOS: 4846
PKM2: 5315"
Figure legend: "PKM2 enzyme kinetics measured with increasing concentrations of phosphoenolpyruvate (PEP) in eNOS immunoprecipitates from HEK293 cells expressing YF or YD eNOS. The data were fit with the Michaelis-Menten equation to determine Vmax and Km; n=4 independent experiments (2-way ANOVA &amp; Bonferroni)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "Quantification of pentose phosphate pathway (PPP) intermediates; gluconate-6-P (G6P), ribulose-5-P (Rl5P), ribose-5-P (R5P), sedoheptulose-7-P (S7P), fructose-6-P (F6P) and erythrose-4-P (E4P) in human endothelial cells expressing YF or YD eNOS; n=6-8 independent cell batches (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "NADPH/NADP+ and GSH/GSSG ratios in human endothelial cells expressing YF or YD eNOS; n=6-8 independent cell batches (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 4846"
Figure legend: "Systolic blood pressure (SBP) in wild-type (WT) and YF-eNOS (YF) mice; n=9 animals per group (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. Phosphorylation of eNOS on Tyr656 in lung lysates from animals treated for 7 days, n=6-7 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. Systolic blood pressure (SBP) in WT and YF mice treated for 28 days, n=10 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. Acetylcholine (ACh)-induced relaxation of aortic rings from the same animals as in panel B, n=8 mice per group (2-way ANOVA and Bonferroni)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. PKM2 S-nitrosation in lungs from WT and YF mice administered AII for 28 days, n=7-8 mice per group (Unpaired Student's t test). DTT treatment was included to demonstrate specificity of the SNO signal. The blots show non adjacent lanes from the same membranes."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. NADPH/NADP+ and GSH/GSSG ratios in lungs from WT and YF mice treated with AII for 28 days; n=8-9 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. 3-Nitrotyrosine (3-NT) levels in lungs from WT and YF mice treated with vehicle (Veh) or AII (AII) for 28 days; n=7-9 mice per group (2-way ANOVA and Bonferroni)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. NADPH/NADP+ and GSH/GSSG ratios in pulmonary endothelial cells from WT and YF mice treated with AII for 30 min; n=8 cell batches (2-way ANOVA and Tukey)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "Wild-type (WT) and YF-eNOS (YF) mice were treated with vehicle (Veh) or angiotensin II (AII, 1.44 mg/kg/day) for up to 28 days. H2O2 levels in pulmonary endothelial cells from WT and YF mice after treatment with AII (1 µmol/L) for 30 min; n=8 cell batches (2-way ANOVA and Tukey)."
NCBI gene IDs:
"eNOS: 18127"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. Proximity ligation assays showing S-nitrosated PKM2 (yellow) in the RCA and LCA 2 days after ligation. Phalloidin staining (blue) was included to highlight the vessel wall; n=4-5 mice per group (2-way ANOVA and Bonferroni). Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. GSH (green) levels in the RCA and LCA 2 days after ligation. CD31 (red) was included to label endothelial cells. n=4-5 mice per group (2-way ANOVA and Bonferroni). Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. 3-nitrotyrosine (3NT, green) levels in the RCA and LCA 2 days after ligation. CD31 (red) was included to label endothelial cells. n=5 mice per group (2-way ANOVA and Bonferroni). Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. Acetylcholine (ACh)-induced relaxation of the phenylephrine contracted LCA 7 days after ligation, n=7-8 mice per group (2-way ANOVA and Bonferroni). Acetylcholine (ACh)-induced relaxation of the phenylephrine contracted aorta 7 days after ligation, n=7-8 mice per group (2-way ANOVA and Bonferroni)."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- mice were treated with vehicle (Veh) or the PKM2 inhibitor shikonin (SKN, 1.2 mg/kg), subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. GSH (green) levels in the RCA and LCA 3 days after ligation. CD31 (red) was included to label endothelial cells. n=5 mice per group (2-way ANOVA and Bonferroni). Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- mice were treated with vehicle (Veh) or the PKM2 inhibitor shikonin (SKN, 1.2 mg/kg), subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. The right, non-ligated artery (RCA) was used as a control. 3-Nitrotyrosine (3NT, green) levels in the RCA and LCA 3 days after ligation. CD31 (red) was included to label endothelial cells. n=5 mice per group (2-way ANOVA and Bonferroni). Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. ICAM1 and VCAM1 (green) expression in the LCA 2 days after ligation. CD31 (red) was included to label endothelial cells and nuclei are highlighted with DAPI (gray). Comparable images were obtained in 4 additional animals in each group. Scale bars: 50 µm."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "ApoE-/- (-/-) and ApoEYF (YF) mice were subjected to partial ligation of the left carotid artery (LCA) and given a cholesterol-rich diet. Percentage of neutrophils and monocytes infiltrated into the LCA 7 days after ligation and high cholesterol diet feeding; n=9-10 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "Percentage of neutrophils and macrophages infiltrated into the LCA from ApoE-/- mice treated with Veh or SKN (1.2 mg/kg) 7 days after ligation and high cholesterol diet feeding; n=4-6 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "Plaque burden in ligated carotid arteries from ApoE-/- (-/-) and ApoEYF (YF) mice fed a high cholesterol diet for 21 days; scale bars: 2 mm. Intimal and medial thickness was determined at four specific locations (proximal, middle 1, middle 2, distal) in cryo-sectioned arteries stained with Oil Red O; scale bars: 200 µm. n=7 mice per group (Unpaired Student's t test)."
NCBI gene IDs:
"ApoE: 11816"
Figure legend: "C. Example of dissected brains (upper panel) and coronal sections (left and right panels) stained with H&amp;amp;amp;E of the indicated genotypes. Note the severe hydrocephaly and ventricular dilation of the lateral ventricle (LV) and third ventricle (TV) in a Gemc1-/- mouse (bottom right panel) compared to a Gemc1+/+ littermate animal (bottom left panel). Periventricular malacia (asterisks) and hippocampal atrophy (arrow) is also evident in the Gemc1-/- sample. Scale bars = 500 µm."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "D. Kaplan Meyer survival curve of a cohort of mice of the indicated genotypes and numbers (n). The study was terminated at 16 months and mice were examined for histology. 97% (31/32) of the Gemc1-/- mice examined to date exhibited hydrocephaly (additional histological examples in Appendix Fig S2)."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "E. RT-QPCR analysis of Gemc1 expression in murine tissues from Gemc1+/+ mice using a Taqman probe. Data is compiled from duplicate samples of 3 male mice per tissue and mean and standard deviation are plotted."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "F. RT-QPCR analysis of Gemc1 in trachea, oviduct and ovary tissue from Gemc1+/+ and Gemc1-/- mice shows that the knockout reduces Gemc1 mRNA to undetectable levels. Mean and standard deviation of duplicate samples from 2 female mice are plotted. Additional examples from other Gemc1-/- tissues are shown in Appendix Fig S1."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "B. Testes from Gemc1-/- mice have strongly reduced cellularity compared to Gemc1+/+ or Gemc1+/- animals, as measured by disaggregation of the tissue and cell counting with a Neubauer Chamber (n=3). Mean and standard deviation are indicated."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "C. Histological analysis of tubules from 6-week-old Gemc1-/- mice revealed thinning of the spermatogenic cell layer and decreased numbers of elongated spermatids. Scale bars = 100 µm (left panels) and 50 µm (right panels)."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "D. Sperm counts from the cauda epididymis of wild type and Gemc1-/- mice revealed no sperm in mice lacking GEMC1 (n=3). Mean and standard deviation are indicated. E. Examples of sperm morphology from spermatocyte spreads from 2 month old mice. Scale bars = 10 µm."
NCBI gene IDs:
"GEMC1: 239789
Gemc1: 239789"
Figure legend: "F and G. Histological evaluation of the caput epididymis in sagittal (F) and coronal (G) orientation from 2 and 4 month old animals, respectively. Note the absence of luminal spermatozoa in the Gemc1-/- mice compared to wild type. Scale bars = 100 µm (F, left panels) and 50 µm (F, right panels and G)."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "A. Examples of ovaries from Gemc1+/+ (top panels) and Gemc1-/- mice (bottom panels) at 6 weeks of age. Ovaries were smaller in Gemc1-/- mice and contained primarily degenerated antral follicles. A histologically normal antral follicle is indicated in the Gemc1+/+ ovary (top right, black arrow) and degenerated antral follicles with high levels of cell death are indicated in the Gemc1-/- ovaries (bottom right, red arrows). Scale bars = 200 µm (left panels)) and 100 µm (right top panels and right bottom panel respectively). B. Morphological abnormalities in the oviduct of 3-week-old Gemc1-/- mice (right panels) compared to Gemc1+/+ (left panels). A clear lack of cilia (bottom panels) in Gemc1-/- oviducts is observed by H&amp;amp;amp;E staining. Scale bars = 50 µm (top panels)."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "C. Staining of frozen sections of oviduct with antibodies for Ac-tubulin (cilia) and γ-tubulin (basal bodies) reveals normal cilia organization in the wild type oviduct (left) while basal bodies and cilia are not detectable in Gemc1-/- oviducts (right). D. FOXJ1 and E. RFX3 staining that is characteristic of MCCs was clearly evident in Gemc1+/+ oviducts and absent in Gemc1-/- mice. Scale bars = 50 µm (C-E)."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "A. H&amp;amp;amp;E staining of the nasal mucosa of E17.5 embryos of the indicated genotype. Normal respiratory pseudostratified columnar ciliated epithelium is shown in Gemc1+/+ embryos compared to the attenuated, flat columnar to squamous epithelium of Gemc1-/- embryos. Scale bars = 20 µm."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "B. PAS staining (purple) of the nasal mucosa of E18.5 embryos of the indicated genotype. Normal PAS positive goblet cells (inset, red arrows) are interspersed between MCCs (inset, black arrows) with their characteristic goblet or cup-like shape in Gemc1+/+ embryos. The Gemc1-/- mucosa has abundant PAS positive materials that accumulate in the apical borders of almost every columnar epithelial cell (inset, red arrows) and lacks typical goblet cells. Scale bars = 20 µm."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "C. Transmission electron micrographs of cross-sections of adult trachea from Gemc1+/+ animals revealed the presence of secretory cells (SC) and multiciliated cells (MCC). The apical membrane of MCCs accumulated basal bodies (BB) nucleating multiple cilia (C) and microvilli (MV). In the Gemc1-/- airway epithelium, no MCCs are identifiable and only secretory-like cells with MV at the apical surface are detectable. Scale bars = 5µm. Higher magnifications of the boxed regions are shown below, scale bars = 0.5µm."
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "E. Cilia (Ac-tubulin staining) are not detectable in the ependymal cells of 2 month old Gemc1-/- mice (right panel). Choroid plexus (CP) and lateral ventricles (LV) are indicated. Scale bars = 20 µm"
NCBI gene IDs:
"Gemc1: 239789"
Figure legend: "E and F. RT-QPCR analysis of individual genes related to MCC formation in the oviduct (E) and trachea (F) of Gemc1-/- mice compared to wild type littermates, using 18S rRNA as a reference (n=2). Genes examined generally fall into 3 classes: centriole duplication (Cep152, Plk4, Cetn2), Deuterosome mediated centriole expansion (Deup1, Ccdc78, Ccno) and transcription of genes required for MCC differentiation (FoxJ1, Myb, Mcidas)."
NCBI gene IDs:
"18S: 
Deup1: 234964
Ccdc78: 381077
Ccno: 218630
Cep152: 99100
Cetn2: 26370
FoxJ1: 15223
Gemc1: 239789
Mcidas: 622408
Myb: 17863
Plk4: 20873"
Figure legend: "A. GEMC1 interacts with E2F4 and DP1. Western blot of HA or FLAG tagged protein expression in the input lysates are shown in the top panel. FLAGimmunoprecipiation (IP) of GEMC1co-IPs DP1 (α-HA, lane 5) and E2F4 (α- HA, lane 6). The interaction is enhanced upon co-expression of HA tagged E2F4 and DP1 (α-HA, lane 7)."
NCBI gene IDs:
"E2F4: 1874
GEMC1: 647309
DP1: 7027"
Figure legend: "B. GEMC1 interacts with E2F5 but not E2F1. Co-IP of E2F5 (α-HA, lane 3) and E2F5-DP1 (α-HA, lane 6) but not E2F1 or E2F1-DP1 (α-HA, lanes 2 and 5). In the FLAG-IP panels, a short (top) and long (second from top) exposure of the Western are shown."
NCBI gene IDs:
"E2F1: 1869
E2F5: 1875
GEMC1: 647309
DP1: 7027"
Figure legend: "C. Identification of the domain required for E2F4 interaction. GFP tagged GEMC1 and mutant forms were expressed and IPs were performed against GFP. Wild type GEMC1 (lane 5) or GEMC1 lacking the CC domain (lane 6) pulled down E2F4-DP1. A GEMC1 protein lacking the TIRT domain or GFP alone did not IP E2F4-DP1 (lanes 7, 8)."
NCBI gene IDs:
"E2F4: 1874
GEMC1: 647309
DP1: 7027"
Figure legend: "D. Mutation of the single amino acid analogous that identified in MCIDAS in human RGMC patients (G313D) impairs the interaction with E2F4-DP1 (compare land 5 and 6)."
NCBI gene IDs:
"MCIDAS: 345643"
Figure legend: "E. GEMC1 interacts with Multicilin through its CC domain. Wild type GEMC1 or a mutant lacking the TIRT domain pull down Myc-tagged Multicilin (anti- Myc blot, lanes 4 and 6) while a CC domain mutant does not (anti-Myc blot, lane 5)."
NCBI gene IDs:
"GEMC1: 647309
Multicilin: 345643"
Figure legend: "A. Transient overexpression of GEMC1 leads to increased levels of FOXJ1 and MCIDAS expression (RT-QPCR) in HEK293T cells. Results for MCIDAS are graphed on a smaller scale in the right panel: V = vector and G = GEMC1. The mean and standard deviation of 3 individual experiments in HEK293T cells are graphed. Similar induction of FOXJ1 was observed in U2OS and T98G (Fig EV3)."
NCBI gene IDs:
"FOXJ1: 2302
GEMC1: 647309
MCIDAS: 345643"