EMBO/SourceData huggingface dataset:
Figure legend: "(c) HeLa cells stably expressing EGFP-LC3 were treated for 4 h with DMSO (control) or 200 nM bafilomycin A1 (Baf), or with 200 nM bafilomycin A1 and 47 μM SMER10, 43 μM SMER18 or 47 μM SMER28. Cells were left untreated or pretreated with SMERs for 24 h before adding bafilomycin A1. Levels of EGFP-LC3-II were determined by immunoblotting with antibody against EGFP (above) and densitometry analysis relative to actin (below). Error bars denote s.e.m. P = 0.0259 (baf), P 0.0001 (SMER10), P = 0.0003 (SMER18 and SMER28) versus control; P = 0.0025 (SMER10), P = 0.0218 (SMER18), P = 0.0195 (SMER28) versus bafilomycin A1. ***P 0.001; **P 0.01; *P 0.05."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "(A)  RT-qPCR measurement of intracellular level of the SARS-CoV-2 RNA  in Huh7  cells after 72 hours and 96 hours of infection. Data information: In A values were normalized to that of the GAPDH  and represented as mean±SD of samples from five independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "(C-J) RT-qPCR analysis of the intracellular mRNA levels of HERV-T (C), HERV-E (D), HERV-R (E), HERV-V (F), HERV-FRD  (G), HERV-MER34  (H), HERV-W (I) and HERV-K-HML-2 (J) in the SARS-CoV-2 infected Huh7  cells, as mentioned in (A). Data information: In C-J, values were normalized to that of the GAPDH  and represented as mean±SD of samples from five independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"HERV-E: 
HERV-MER34: 
GAPDH: 2597"
Figure legend: "(A) RT-qPCR  measurement of intracellular level of the SARS-CoV-2 RNA  (normalized to that of the GAPDH) in  Calu 3 cells after 48 hours and 72 hours of infection. Data information: In A values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "(C-J) RT-qPCR analysis of the intracellular mRNA levels of HERV-T  (C), HERV-E (D), HERV-R (E), HERV-V (F), HERV-FRD (G), HERV-MER34 (H), HERV-W (I) and HERV-K-HML-2 (J) in the SARS-CoV-2 infected  Calu 3  cells, as mentioned in (A). Data information: In C-J, values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"HERV-E: 
HERV-MER34: 
GAPDH: 2597"
Figure legend: "(B)  RT-qPCR measurement of exon 1 region [shown in (A)] transcript in Huh7  cells infected with SARS-CoV-2 for 72hours. (C) RT-qPCR  measurement of exon 1 region transcript in  Calu 3  cells infected with SARS-CoV-2 for 72hours. Data information: In B and C, values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "(E) RT-qPCR measurement of  HERV-R-Env  transcript level in PBMCs  isolated from 19 healthy individuals and 16 COVID-19 patients. (F) RT-qPCR measurement of Exon1 region transcript level in PBMCs  isolated from 19 Healthy individuals and 16 COVID-19 patients. Data information: values were normalized to that of the GAPDH  and represented as mean±SD of samples from 19 healthy and 16 COVID-19 patients, assayed in duplicate."
NCBI gene IDs:
"Env: 
GAPDH: 2597"
Figure legend: "(G) Quantitation of HERV-R-Env  RNA level in transcriptome dataset of indicated human lung  tissues Data information: In G, HERV-R-Env  copy number in two biological replicates (Healthy lung) and two technical replicates (COVID-19 lung) of the transcriptome dataset are represented as mean±SD."
NCBI gene IDs:
"Env: "
Figure legend: "(H) Quantitation of HERV-R-Env  RNA level in transcriptome dataset of Calu 3 cells, analyzed at 4, 8, 12 and 24 hours of SARS-CoV-2  infection, as indicated. Data information: In H, HERV-R-Env  copy number in three biological replicates of the transcriptome dataset are represented as mean±SD. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"Env: "
Figure legend: "(A) RT-qPCR measurement of intracellular level of the  SARS-CoV-2 delta variant  RNA  in Huh7  and Calu 3  cells after 24, 48 and 72 hours of infection. (B) RT-qPCR measurement of intracellular level of the SARS-CoV-2 omicron variant RNA  in Huh7  and  Calu 3  cells after 24, 48 and 72 hours of infection. Data information: values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "(C) RT-qPCR analysis of the HERV-R-Env  RNA level in the SARS-CoV-2 delta variant infected Huh7  and Calu 3  cells, as mentioned in (A). (D)  RT-qPCR analysis of the HERV-R-Env  RNA level in the SARS-CoV-2 omicron variant infected Huh7  and Calu 3 cells, as mentioned in (B). Data information: values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"Env: 
GAPDH: 2597"
Figure legend: "(F) RT-qPCR  measurement of intracellular level of the SARS-CoV-2 RNA  (normalized to that of the GAPDH) in Huh7  cells transfected with NT-siRNA (Non target siRNA) or HERV-R-Env  siRNA and infected with the SARS-CoV-2 for 24, 48 or 72 hrs, as indicated. Values are mean±SD of 3 independent experiments. Data information: In F, values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments."
NCBI gene IDs:
"Env: 
GAPDH: 2597"
Figure legend: "(G) Dual luciferase assay showing activation of IFNγ, ISRE, ISG56  and NFκB  promoters in  HEK 293T cells expressing the empty vector (V), constitutively active mutant of RIG-I, R-C, 25 and 50 ng of  HERV-R-Env. Activation of these promoters by R-C was taken as 100%. Data information: In G, FF Luc  values were normalized to that of the RL Luc  and represented as % mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"Env: 
Luc: 
ISG56: 3434
NFκB: 4790
RIG-I: 23586"
Figure legend: "(H) Western blot  analysis of the indicated proteins in Huh7  cells, transiently transfected with HERV-R-Env  expression plasmid."
NCBI gene IDs:
"Env: "
Figure legend: "(B) HERV-R 5'-LTR activity in Huh7  cells cotransfected with the pGL3 Fl 5'-LTR G-Luc  and pGL3 promoter Firefly-Luc  (denoted as Fl 5'-LTR  G-Luc) or pGL3  G-Luc  and pGL3 promoter Firefly-Luc  (denoted as G-Luc) plasmids for 48 hours. (C) HERV-R  5'-LTR activity in Huh7  cells cotransfected with the pGL3 Fl 5'-LTR G-Luc   and pGL3 promoter Firefly-Luc  (denoted as Fl 5'-LTR  G-Luc) or pGL3 ∆ 5'-LTR G-Luc  and pGL3 promoter Firefly-Luc  (denoted as ∆ 5'-LTR G-Luc) or pGL3 G-Luc  and pGL3 promoter Firefly-Luc  (denoted as  G-Luc) plasmids for 48 hours. Data information: G-Luc   values were normalized to that of the Firefly-Luc  and represented as mean±SD of samples from three independent experiments."
NCBI gene IDs:
"G-Luc: 
Luc: "
Figure legend: "(I) RT-qPCR measurement of HERV-R-Env  RNA in Huh7  cells infected with the SARS-CoV-2  and treated with the indicated compounds for 48 hours. Data information: In I values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"Env: 
GAPDH: 2597"
Figure legend: "(K)  RT-qPCR measurement of intracellular level of the SARS-CoV-2 RNA  in Huh7  cells infected with the  SARS-CoV-2 and treated with the indicated compounds for 48 hours. Data information: In K, values were normalized to that of the GAPDH  and represented as mean±SD of samples from three independent experiments. Student's t-test was applied to determine the significance between the groups."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "G. Schematic diagram of human TRIM50  (WT) and its truncation mutants was presented(top), and the interaction  of TRIM50   (TRIM50   truncation mutants) and NLRP3  was detected by IP  assay. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"TRIM50: 135892"
Figure legend: "H, I. Schematic diagram of human NLRP3  (WT) and its truncation mutants (top), and immunoprecipitation  assay of the interaction  of NLRP3  (NLRP3  truncation mutants) with TRIM50  in HEK293T  cells. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548"
Figure legend: "A. Immunoblot  analysis of the NLRP3  protein level in the mouse peritoneal macrophages  transfected with Si-Trim50. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-tailed Student's t-test (A, Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "B.  qRT-PCR analysis of Nlrp3  and IL-1β mRNA levels in LPS-primed Si-Trim50  transfected mouse peritoneal macrophages. Data information: P-values are shown, two-way analysis of variance (ANOVA) (B, Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"IL-1β: 16176
Nlrp3: 216799
Trim50: 215061"
Figure legend: "C, D. Immunoblot  analysis of Caspase1-P20 (C) and  Gasdermin D (D) of WT or Trim50-/- mouse peritoneal macrophages after LPS  stimulation for 6h and ATP  treatment for 30min. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "E, Immunoblot  analysis of  Caspase1-P20 (E) of WT or Trim50-/- BMDM  cells after LPS  stimulation for 6h and ATP  treatment for 30min. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "F. Immunoblot  analysis of Gasdermin D  (F) of WT or Trim50-/- BMDM  cells after LPS  stimulation for 6h and ATP  treatment for 30min. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "G, ELISA  analysis of  IL-1β, TNF-α and IL-6 in the supernatant of WT or Trim50-/- peritoneal macrophages with the activation of NLRP3  inflammasome. n=3 Data information: P-values are shown, two-way analysis of variance (ANOVA) G, Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "H. ELISA  analysis of  IL-1β, TNF-α  and  IL-6 in the supernatant of WT or Trim50-/- BMDM  cells with the activation of NLRP3  inflammasome. n=3 Data information: P-values are shown, two-way analysis of variance (ANOVA) Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "I. LDH  analysis of WT or Trim50-/- mouse peritoneal macrophages  with the activation of NLRP3  inflammasome. n=3 Data information: P-values are shown, two-way analysis of variance (ANOVA) I). Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "A. Immunoprecipitation  analysis of the NLRP3 ubiquitination  in HEK293T  cells transfected with HA-ubiquitin, His-NLRP3  and Flag-TRIM50   plasmids. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Flag: 
HA: 
His: 
NLRP3: 114548
ubiquitin: 6233///7311///7316///7314
TRIM50: 135892"
Figure legend: "B. Immunoprecipitation  analysis of the endogenous NLRP3 ubiquitination  in WT or Trim50-/- BMDM  cells. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "C. Immunoprecipitation  analysis of NLRP3 ubiquitination  in HEK293T  cells transfected with NLRP3, HA-Ubiquitin, TRIM50   or TRIM50   (ΔRING) mutant plasmids. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"HA: 
NLRP3: 114548
Ubiquitin: 6233///7311///7316///7314
TRIM50: 135892"
Figure legend: "F. Immunoblot  analysis of NLRP3  in HEK293T  cells transfected with NLRP3  plasmid and TRIM50  or TRIM50   (ΔRING) mutant, followed with the further treatment with CHX  for 0h, 4h or 8h. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-way ANOVA Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "A. SDD-AGE analysis of NLRP3 oligomerization  in HEK293T cells transfected with NLRP3  and TRIM50   plasmids. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "B.  SDD-AGE  analysis of NLRP3 oligomerization  in NLRP3  and TRIM50   transfected HEK293T  cells followed with nigericin  treatment for 30min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "C. Immunoblot  analysis of ASC oligomerization  in cross-linked cytosolic  pellets (insoluble) and whole cell lysate (soluble) in HEK293T  cells transfected with NLRP3, ASC  and TRIM50   plasmids followed with nigericin   treatment for 30 min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
ASC: 29108
TRIM50: 135892"
Figure legend: "D. SDD-AGE analysis of NLRP3 oligomerization  in WT or Trim50-/- peritoneal macrophages  with LPS  treatment for 6h and ATP  treatment for 30 min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "E. Immunoblot  analysis of ASC oligomerization  in cross-linked cytosolic  pellets (insoluble) and whole  cell lysate (soluble) in WT or Trim50-/- peritoneal macrophages by LPS  stimulation for 6h and ATP  treatment for 30min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "F. SDD-AGE  analysis of NLRP3 oligomerization  in WT or Trim50-/- BMDM  cells with LPS  treatment for 6h and ATP  treatment for 30 min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "G. Immunoblot  analysis of ASC oligomerization  in cross-linked cytosolic  pellets (insoluble) and whole  cell lysate (soluble) in WT or Trim50-/- BMDM  cells by LPS  stimulation for 6h and ATP  treatment for 30min. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "H. SDD-AGE  analysis of the oligomerization   of NLRP3  in HEK293T  cells transfected with His-NLRP3  and TRIM50   or TRIM50(ΔRING) mutant. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"His: 
NLRP3: 114548
TRIM50: 135892"
Figure legend: "J. Immunoprecipitation  analysis of NLRP3 ubiquitination  in HA-ubiquitin, His-NLRP3  and Myc-TRIM50  transfected HEK293T  cells with further treatment with tranilast  or MCC950  for 24h. Data information: Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"His: 
Myc: 
NLRP3: 114548
TRIM50: 135892"
Figure legend: "A. Immunoprecipitation  analysis of NLRP3 ubiquitination  in HEK293T  cells transfected with NLRP3, HA-Ubiquitin, TRIM50   or its truncation mutants. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "B. SDD-AGE analysis of NLRP3 oligomerization  in NLRP3   together with TRIM50   or its coiled-coil domain-deleted mutants (ΔC-C1 and ΔC-C2) transfected HEK293T  cells. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "C. SDD-AGE  analysis of the oligomerization  of TRIM50   in TRIM50   or its coiled-coil domain-deleted mutants (ΔC-C1 and ΔC-C2) transfected HEK293T  cells. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"TRIM50: 135892"
Figure legend: "D. SDD-AGE analysis of the oligomerization  of NLRP3  and TRIM50   in HEK293T  cells overexpressing NLRP3  together with TRIM50   or its coiled-coil domain-deleted mutants. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "E. Immunoblot  analysis of NLRP3  in HEK293T  cells transfected with NLRP3  plasmid and TRIM50   coiled-coil domain-deleted mutants, followed with the cycloheximide  (CHX) treatment for 0h, 4h or 8h. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. P-values are shown, two-way ANOVA(E). Data are representative of three biological replicates with similar results. (mean ± SD in E)."
NCBI gene IDs:
"NLRP3: 114548
TRIM50: 135892"
Figure legend: "F. Immunoblot  analysis of ASC oligomerization  in cross-linked cytosolic  pellets (insoluble) and whole cell lysate(soluble) in HEK293T  cells transfected with His-NLRP3  and TRIM50   or TRIM50   coiled-coil domain-deleted mutants. Data information: Band densities were quantitated by 'Image J' software and normalized to GAPDH. Data are representative of three biological replicates with similar results."
NCBI gene IDs:
"His: 
NLRP3: 114548
TRIM50: 135892"
Figure legend: "A. Survival  status of WT and Trim50-/- mice  after intraperitoneal injection with LPS  (n=10 mice/group). Data information: P-values are shown, two-way ANOVA (A) Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "B. ELISA  analysis of  IL-1β, TNF-α and IL-6 in serum  of WT or Trim50-/- mice  after i.p. injection with LPS  for 6h (n=4-6 mice/group). Data information: P-values are shown, two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "D. Flow cytometry analysis of the neutrophils  (left) and  Ly6C+ monocytes  (right) in peritoneum  of WT or Trim50-/- mice  after i.p. injection with Alum. (n=6 mice/group). Data information: P-values are shown, two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "E. WT or Trim50-/- mice  were i.p. injected with FA  for 36h, followed with immunoblot  analysis of NLRP3, pro-IL-1β, Caspase1 p20, IL-1β in the kidney  tissue samples. Data information: P-values are shown, two-way ANOVA (A) or two-tailed Student's t-test Data are representative of three biological replicates with similar results. (mean ± SD"
NCBI gene IDs:
"Trim50: 215061"
Figure legend: "B-C') Immunofluorescence analysis of active-caspase 3 in the ONL of P347S and P347S/miR-181a/b+/-. D, E) TUNEL analysis in the ONL of P347S and P347S/miR-181a/b+/-. F) Quantification of immunofluorescence analysis in (B-C') (N=4 eyes P347S and N=6 eyes P347S/ miR-181a/b+/). G) Quantification of TUNEL analysis in (D, E) (N=3 eyes/genotype)."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "D-P) Immunofluorescence analysis showed amelioration of Rhodopsin localization (D-F'; white arrowheads); C-arrestin expression (G-I') and PR outer segment (OS) and inner segment (IS) structures, as determined, respectively, by PNA (J-L'); and Phalloidin staining (M-O'') in P347S/miR-181a/b+/- vs P347S eyes at p30. M''-O'' show higher magnification of M-O. Scale bars 25 μm in D-O'; 5μm in M''-O''. Fluorescence densitometry quantification of each staining is reported in (P), N=3 eye/genotype for each staining. WT vs P347S p values are reported in red, P347S vs P347S/miR-181a/b+/- p values are reported in black."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "D-L) Immunofluorescence analysis of Rhodopsin (D-E'), C-arrestin (F-G'), PNA (H-I'); and Phalloidin staining (J-K'') in P347S and P347S/miR-181a/b+/- retinas at p90. J''-K'' show higher magnification of J-K. Scale bars 25 μm in D-K'; 10μm in J''-K''."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "Immunofluorescence analysis of Rhodopsin C-arrestin PNA and Phalloidin staining in P347S and P347S/miR-181a/b+/- retinas at p90. Fluorescence densitometry quantification of each staining is reported in (L), N=3 eye/genotype/staining."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "M) Measurement of P347S and P347S/miR-181a/b+/- ONL thickness (N≥3 eyes/genotype;"
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "N) Graphical representation of the results of OKR analysis by the optokinetic tracking assays reported as cycles/degree. Visual acuity is preserved in P347S/miR-181a/b+/- animals with respect to P347S (N=4 animals/genotype)."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "B-F) Electron microscopy analysis shows an increase of mitochondrial fragmentation in P347S PRs with respect to WT and amelioration of the mitochondrial phenotype in P347S/miR-181a/b+/- vs P347S PRs at p12 and p30 (N=2 animals/genotype). M, mitochondria. G-I) The quantitative analysis of mitochondrial phenotype is expressed as measurement of the mitochondria perimeter (G), mitochondria number (H) and as the ratio of mitochondria area per the analyzed field area (I). N=2 animals/genotype."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "A) WB analysis of Drp1, one of the key proteins involved in the mitochondrial fission pathway, in the optic cup of WT, P347S DMSO-treated, P347S/miR-181a/b+/- DMSO-treated and of P347S/miR-181a/b+/- FEDRATINIB-treated (an inhibitor of the JAK2/STAT3 pathway) mice at p30."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "B) Quantification revealed that Drp1 protein levels are increased in P347S with respect to WT, and partially rescued in P347S/miR-181a/b+/-. Data are normalized to Gapdh. N ≥ 4 eye/genotype. Please note that all compared bands from WT, P347S, P347S/miR-181a/b+/- and P347S/miR-181a/b+/- with FEDRATINIB samples are from the same blots, which were cropped and shown organized in the panel for the sake of data presentation clarity (see source data)."
NCBI gene IDs:
"miR-181a: 735252"
Figure legend: "C-D) qRT-PCR analysis reveals decreased levels of the Drp1 transcript and increased levels of Stat3 and Jak2 transcripts in the eyes of P347S/miR-181a/b+/- vs those of P347S animals. N≥4 eyes/genotype."
NCBI gene IDs:
"Drp1: 74006
Jak2: 16452
miR-181a: 735252
Stat3: 20848"