EMBO/SourceData huggingface dataset:
Figure legend: "Retinalmicroglia were depleted in rd10/CreDTAmice by the oral administration of tamoxifen (in corn oil) to activate microglia-specific Cre-mediated recombination and diphtheria toxin expression; control CreDTA littermates were administered corn oil without tamoxifen.A-D Depletion of retinalmicroglia in the rd10 retina. Representative retinal section from a P28 control animal demonstrates Iba1+microglia in the retina, including those infiltrating the ONL (A), while a tamoxifen-administered littermate (B) was substantially depleted of retinalmicroglia. Scale bar, 40 μm. Microglial cell counts in the entire retina (C) and in the ONL only (D) confirmed efficient depletion of infiltrating microglia following tamoxifen administration (n = 8 control and 11 depleted animals from four litters, two-sided unpaired t-test).E-J Effect of microglial depletion on retinal degeneration at P28-29. ONL atrophy and thinning in control animals (E) was significantly more advanced relative to microglia-depleted littermates (F). Scale bar, 40 μm. Quantification of mean ONL thickness (G) and mean number of layers of ONLnuclei (H) at P28-29 demonstrate significantly greater ONL preservation in depleted retinas; the degree of ONL preservation correlated with the extent of microglia depletion (I). (J) The mean density of TUNEL+nuclei in the ONL was not significantly decreased in depleted vs. control animals (n = 8 control and 11 depleted animals from four litters, two-sided unpaired t-test).K-N Continuation of microglial depletion until P37-39 resulted in the persistence of morphological rescue (K-M), with a significant reduction in TUNEL+nuclei density (N) (n = 6 control and nine depleted animals from two litters, two-sided unpaired t-test).O-R Similar rescue effects as in (K-N) remained apparent when depletion was sustained until P50 (O-R), a time when rod degeneration in the rd10 model is relatively complete (n = 9 control and 10 depleted animals from three litters, two-sided unpaired t-test).S, T Functional rescue of photoreceptors was evident following microglial depletion until P50 in significantly increased dark- (S) and light-adapted (T) responses in ERG testing in depleted animals (green lines) relative to control animals (black lines), in both a- and b-wave amplitudes across multiple flash intensities (n = 9 control and nine depleted animals, *P &lt; 0.05 in one-way ANOVA with Sidak's multiple comparison test)."
NCBI gene IDs:
"Cre: "
Figure legend: "Depletion of infiltrating microglia in the rd10 retina decreases IL-1β levels. Cytokine levels in rd10/CreDTA mouse retinas were assayed following tamoxifen-induced microglial depletion (from P21 to P37-50, green bars; n = 9 animals) and compared with their untreated littermate controls (white bars, n = 8 animals; values normalized to control animals in the same litter). IL-1β protein levels were significantly lowered following microglial depletion, but IL-6, CCL2, or TNFα were not significantly changed."
NCBI gene IDs:
"Cre: "
Figure legend: "Quantification of human EGFL7 by qRT-PCR in primary GBM biopsies and corresponding PDX xenografts upon implantation of GBM-derived organotypic spheroids (n = 3; Mann-Whitney U-test; mean ± SEM); B - GBM biopsy; G - generation."
NCBI gene IDs:
"EGFL7: 51162"
Figure legend: "Alignment of EGFL7 expression along with stromal (VWF, VEGFR1, VEGFR2, ACTA2, CD248, ITGAX, AIF1, CD68, CD4, and PTPRC) or tumor markers (EGFR, PDGFRA, VEGFA, VIM, and NES) in primary GBM biopsies or GBM-derived organotypic spheroids (PDX xenografts)."
NCBI gene IDs:
"ACTA2: 59
AIF1: 199
CD248: 57124
CD4: 920
CD68: 968
EGFL7: 51162
EGFR: 1956
VEGFR1: 2321
ITGAX: 3687
VEGFR2: 3791
NES: 10763
PDGFRA: 5156
PTPRC: 5788
VEGFA: 7422
VIM: 7431
VWF: 7450"
Figure legend: "Overexpression of human EGFL7 (hE7) or murine EGFL7 (mE7) increased tumor growth (n = 9; one-way ANOVA)."
NCBI gene IDs:
"EGFL7: 51162
EGFL7: 353156
hE7: 51162
mE7: 353156"
Figure legend: "Immunocompetent mice bearing GL261 tumors. Representative MRI images confirmed tumor implantation in all experimental groups (left). Kaplan-Meier curves revealed decreased survival of mice bearing EGFL7 expressing tumors (right; 34 d (control) vs. 29.5 d (hE7) vs. 31.5 d (mE7); n = 8; log-rank test). Immunodeficient mice bearing U87 tumors. Representative magnetic resonance images confirmed tumor implantation in all experimental groups (left). Kaplan-Meier curves revealed decreased survival of mice bearing EGFL7 expressing tumors (right; 70 d (control) vs. 42.5 d (hE7) vs. 40 d (mE7); n = 8; log-rank test)."
NCBI gene IDs:
"EGFL7: 51162
EGFL7: 353156
hE7: 51162
mE7: 353156"
Figure legend: "miR-126 KO did not affect overall survival of GL261 tumor-bearing mice (n = 8)."
NCBI gene IDs:
"miR-126: 387145"
Figure legend: "EGFL7 KO prolonged overall survival of GL261 tumor-bearing mice as compared to wild-type (WT) littermates (44 d in KO vs. 35.5 d in WT; n = 8 each; log-rank test)."
NCBI gene IDs:
"EGFL7: 353156"
Figure legend: "Residual EGFL7 expression in BTPC11 glioma cells was reduced by a lentivirus-based approach. Knock-down of EGFL7 (shE7_1 or shE7_2) prolonged the median survival time of glioma-bearing mice (68 d or 69 d) as compared to scrambled control (shScr; 56 d; n = 6; log-rank test)."
NCBI gene IDs:
"EGFL7: 51162"
Figure legend: "The blood vessel-specific and miR126-independent KO of EGFL7 in EGFL7fl/fl;Cdh5-CreERT2 mice prolonged the overall survival of GL261 tumor-bearing mice as compared to WT littermates (38 d in KO vs. 33 d in WT; n = 7 for KO; n = 5 for WT; log-rank test). All data are presented as mean ± SEM. Scale bars represent 5 mm."
NCBI gene IDs:
"Cdh5: 12562
Cre: 2777477
EGFL7: 353156
ERT2: 2099
miR126: 387145"
Figure legend: "CD31 staining for endothelial cells revealed increased tumor vascularization in mice bearing human EGFL7 (hE7)- or murine EGFL7 (mE7)-positive tumors (n = 3; one-way ANOVA)."
NCBI gene IDs:
"EGFL7: 353156
hE7: 51162
mE7: 353156"
Figure legend: "T2-weighted magnetic resonance imaging (MRI) analysis of these brains confirmed that all mice developed tumors of similar size. T1-weighted MRI images showed decreased vessel permeability as measured by Gadovist extravasation in tumors expressing hE7 or mE7 (red arrows; n = 6; one-way ANOVA). Scale bar represents 2.5 mm."
NCBI gene IDs:
"hE7: 51162
mE7: 353156"
Figure legend: "EGFL7 promoted density and maturation state of glioma vessels Enhanced maturation of glioma vessels in the presence of hE7 or mE7 was verified by increased co-localization of PDGFRβ (pericytes) with CD31 (n = 3; one-way ANOVA; quantifications normalized to CD31). Data presented as mean ± SEM, AU-arbitrary units. Scale bars represent 60 µm."
NCBI gene IDs:
"EGFL7: 51162
hE7: 51162
mE7: 353156"
Figure legend: "Enhanced maturation of glioma vessels in the presence of hE7 or mE7 was verified by increased co-localization of SMA (smooth muscle cells), with CD31 (n = 3; one-way ANOVA; quantifications normalized to CD31). Data presented as mean ± SEM, AU-arbitrary units. Scale bars represent 60 µm."
NCBI gene IDs:
"hE7: 51162
mE7: 353156"
Figure legend: "Enhanced maturation of glioma vessels in the presence of hE7 or mE7 was verified by increased co-localization of (I+J) Col IV (basement membrane) with CD31 (n = 3; one-way ANOVA; quantifications normalized to CD31). Data presented as mean ± SEM, AU-arbitrary units. Scale bars represent 60 µm."
NCBI gene IDs:
"hE7: 51162
mE7: 353156"
Figure legend: "Ectopic expression of murine EGFL7 (mE7) in GL261 mouse glioma cells followed by intracranial implantation into the striatum of immune-competent mice reduced overall survival but was rescued upon treatment with an integrin α5β1-inhibiting antibody (n = 6; log-rank test). Data presented as mean ± SEM. TCL-total cell lysate, AU-arbitrary units."
NCBI gene IDs:
"EGFL7: 353156
mE7: 353156"
Figure legend: "Treatment with anti-EGFL7 (47 d), anti-VEGF (51 d), or a combination of both antibodies increased the median survival time of glioma-bearing Rag1-/- mice (58 d) as compared to isotype-treated controls (37 d; n = 6; log-rank test)."
NCBI gene IDs:
"Rag1: 19373"
Figure legend: "B) m6A dot blot of Raji cells that expressed NTC or MYC shRNAs (B); equal mRNA loading was verified by methylene blue staining. The shown data are representative of at least three independent experiments."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(C) Quantification of m6A abundance by HPLC-MS. *** P&lt;0.001 as compared to corresponding high MYC group (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(G) m6A-RIP assay in P493-6 cells treated with Tet for 0h or 72h. HPRT1 serves as negative control. *** P&lt;0.001 as compared to corresponding high MYC group, ns, not significant (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251
MYC: 4609"
Figure legend: "I) Western blot analysis for protein levels in Raji cells that expressed NTC or MYC shRNAs (I). HPRT1 and β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "B) Western blot analysis for protein levels of m6A methyltransferases (METTL3 and METTL14) and demethylases (ALKBH5 and FTO) in Raji cells that expressed NTC or MYC shRNAs (B). β-actin serves as loading controls. Data are representative of at least three independent experiments."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(D) IGV graph showing location of MYC binding peaks on ALKBH5 and FTO from published ChIP-seq datasets (Walz et al, 2014; Data ref: Eilers M, 2014). In this dataset, high levels of MYC were induced with 1µg/ml doxycycline for 30h and ChIPed by MYC antibody. Red triangle indicates the E-box."
NCBI gene IDs:
"ALKBH5: 54890
FTO: 79068"
Figure legend: "(E) RIP assay, using ALKBH5, FTO or IgG antibody to detect the binding to MRGs (SPI1 and PHF12) in P493-6 cells treated with Tet or not. HPRT1 serves as negative control. *** P&lt;0.001 as compared to corresponding IgG group (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251
PHF12: 57649
SPI1: 6688"
Figure legend: "(F Western blot analysis for protein levels in P493-6 cells that overexpressed empty vector (EV), ALKBH5, or FTO and were then treated with Tet or not (F) HPRT1 and β-actin served as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"ALKBH5: 54890
FTO: 79068"
Figure legend: "G) Western blot analysis for protein levels in Raji cells expressed NTC or MYC shRNAs (G). HPRT1 and β-actin served as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(H) m6A RIP assay in P493-6 cells that overexpressed EV, ALKBH5, or FTO and were then treated with Tet or not. HPRT1 serves as negative control. *** P&lt;0.001 as compared between indicated groups. ns, not significant (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"ALKBH5: 54890
FTO: 79068
HPRT1: 3251"
Figure legend: "(I, Western blot analysis for protein levels in P493-6 cells that expressed NTC or ALKBH5 shRNAs and were then treated with Tet or not (I) HPRT1 and β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"ALKBH5: 54890"
Figure legend: "J) Western blot analysis for protein levels in Raji cells expressed NTC or ALKBH5 shRNAs and knocked down MYC or not (J). HPRT1 and β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"ALKBH5: 54890
MYC: 4609"
Figure legend: "(K) m6A RIP assay HPRT1 serves as negative control. *** P&lt;0.001 as compared between indicated groups (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251"
Figure legend: "(A) RIP assay in P493-6 cells treated with Tet for 0h or 72h. HPRT1 serves as negative control. *** P&lt;0.001 relative to indicating groups (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251"
Figure legend: "(B, Western blot analysis for protein levels in P493-6 cells that expressed NTC or YTHDF3 shRNAs and were then treated with Tet or not (B) HPRT1 and β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"YTHDF3: 253943"
Figure legend: "C) Western blot analysis for protein levels in Raji cells that expressed NTC or YTHDF3 shRNAs and knocked down MYC or not (C). HPRT1 and β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"MYC: 4609
YTHDF3: 253943"
Figure legend: "(D) Polysomes profiling assay in P493-6 cells that expressed NTC or YTHDF3 shRNA."
NCBI gene IDs:
"YTHDF3: 253943"
Figure legend: "(E) RT-qPCR assay for mRNA levels of MRGs (SPI1 and PHF12) in different fractions in (D). HPRT1 serves as negative control. Data were presented as mean (±SD), n = 3 biological replicates. ** P&lt;0.01 and *** P&lt;0.001 as compared to NTC group (Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251
PHF12: 57649
SPI1: 6688"
Figure legend: "(F) Click-iT AHA (L-azidohomoalaine) experiments were performed using IgG, anti-SPI1 or anti-PHF12 antibody. P493-6 cells expressing NTC or YTHDF3 shRNAs were incubated for 1 hour in medium containing 100 ug/mL AHA. The translated proteins were detected by Western blot. Arrow indicates translated MRGs."
NCBI gene IDs:
"YTHDF3: 253943"
Figure legend: "H) Western blot analysis for protein levels in Raji cells (H) that expressed NTC, or ALKBH5 shRNA, or YTHDF3 shRNA. β-actin serve as negative and loading controls, respectively. Data are representative of at least three independent experiments."
NCBI gene IDs:
"ALKBH5: 54890
YTHDF3: 253943"
Figure legend: "(I) m6A RIP assay HPRT1 serves as negative control. *** P&lt;0.001 as compared between indicated groups, ns, not significant (mean ± SD, n = 3 biological replicates, Student's t‐test)."
NCBI gene IDs:
"HPRT1: 3251"
Figure legend: "(A) Trypan blue counting was used to analyze growth curves for P493-6 cells that expressed NTC or SPI1 shRNA or PHF12 shRNA. ** P&lt;0.01 as compared between indicated groups. (mean ± SD, n = 4 biological replicates, Student's t‐test)"
NCBI gene IDs:
"PHF12: 57649
SPI1: 6688"
Figure legend: "(B, C) Trypan blue counting was used to analyze growth curves for P493-6 cells overexpressing ALKBH5 and further infected with viruses expressing SPI1 (B) or PHF12 (C). ** P&lt;0.01 as compared between indicated groups (mean ± SD, n = 4 biological replicates, Student's t‐test)."
NCBI gene IDs:
"ALKBH5: 54890
PHF12: 57649
SPI1: 6688"
Figure legend: "(D, E) Raji cells stably expressing EV or MYC were infected with viruses expressing shALKBH5. Cells were injected subcutaneously into nude mice (n=5 for each group). Tumor growth curves were measured starting from 12 days post injection (D). Photo of tumors collected at the end of the experiment (day 27) (E). Data are presented as mean (±SEM). ** P&lt;0.01 or *** P&lt;0.001 as compared between indicated groups (Student's t‐test)."
NCBI gene IDs:
"ALKBH5: 54890
MYC: 4609"
Figure legend: "(F P493-6 cells stably expressing NTC or shSPI1 or shPHF12 were injected subcutaneously into nude mice (n=5 for each group). Tumor growth curves were measured starting from 12 days post injection (F). Data are presented as mean (±SEM). ** P&lt;0.01 as compared between indicated groups (Student's t‐test)."
NCBI gene IDs:
"PHF12: 57649
SPI1: 6688"
Figure legend: "G) P493-6 cells stably expressing NTC or shSPI1 or shPHF12 were injected subcutaneously into nude mice (n=5 for each group). Photo (G) of tumors collected at the end of the experiment (day 25)."
NCBI gene IDs:
"PHF12: 57649
SPI1: 6688"
Figure legend: "(H, I) P493-6 cells stably expressing EV or ALKBH5 were infected with viruses expressing SPI1 or PHF12. Cells were injected subcutaneously into nude mice (n=5 for each group). Tumor growth curves were measured starting from 17 days post injection (H). Photo (I) of tumors collected at the end of the experiment (day 32). Data are presented as mean (±SEM). * P&lt;0.05 as compared between indicated groups (Student's t‐test)."
NCBI gene IDs:
"ALKBH5: 54890
PHF12: 57649
SPI1: 6688"
Figure legend: "A. Trem2  CV and Trem2  R47H KI primary microglia treated simultaneously with oAβ-bound synaptosomes  (SN) conjugated with pHrodo red  (magenta) and control SN, conjugated with pHrodo deep red (cyan). Scale bar, 50 μm. B. pHrodo  fluorescence with time shown as AUC at 3 h. AUC of oAβ-SN  is higher compared to Ctrl-SN  in Trem2  CV but not in Trem2  R47H KI microglia. ∼40 microglia  per ROI, 2 ROIs per well, 2-3 wells per experiment, n=3 independent experiments. C. Quantification of the percent of C1qa  area covered normalized to WT showing an increase in the  NL-F but not NL-F KI; Trem2  R47H KI. N=3-4 animals per genotype. Data information: Data shown as mean ± SEM. Each shaded point represents 1 ROI, and each open point represents the mean of each independent experiment. Two-way ANOVA followed by Bonferroni's post-hoc test. P-values shown as ns P&gt;0.05; *P&lt;0.05; ***P&lt;0.001; ****P&lt;0.0001."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "Hippocampal CA1 SR  of 6 mo WT, Trem2  R47H KI, NL-F KI and NL-F KI; Trem2  R47H KI mice immunostained  for P2Y12  (red), CD68  (magenta) and Homer1 (green). G. Representative 3D surface rendering reconstructions showing increased Homer1  in microglia  in NL-F KI compared to WT, Trem2 R47H KI, and NL-F KI; Trem2  R47H KI mice. Scale bar, 10 μm."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "I. SRM  images from the hippocampal CA1 SR   of 6 mo WT, Trem2  R47H KI, NL-F KI and NL-F KI; Trem2  R47H KI mice immunostained  for Synaptotagmin (Syt1/2, magenta) and Homer1  (green), pre- and  post-synaptic   puncta, respectively. Scale bar, 1 μm."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "B. Percentage of synaptic Homer1-immunoreactive puncta found  co-localized  with PSVue  in NL-F KI; Trem2  R47H compared to NL-F KI. 3 ROIs per animal, n=3-4 animals per genotype. Data information: Data shown as mean ± SEM. Unpaired t-test (B) P-values shown as ns P&gt;0.05; *P&lt;0.05; **P&lt;0.01, ****P&lt;0.0001."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "D. Number of spontaneous calcium  transients per minute at 48 h post-treatment of Aβ  oligomer in neuron-only culture (cyan), neuron- Trem2  CV microglia  co-culture (magenta) or neuron- Trem2  R47H KI microglia  co-culture (purple). ∼10 spines  per neuron, ∼10 neurons  per experiment, from 3-4 independent experiments. Data information: Data shown as mean ± SEM. Each shaded point represents 1 ROI, and each open point represents the mean (or median for GCaMP  studies) of each independent experiment. Central bands of the violin plot (D) represent median and quartiles. Kruskal-Wallis test followed by Dunn's test (D), P-values shown as ns P&gt;0.05; *P&lt;0.05; **P&lt;0.01, ****P&lt;0.0001."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "F. SRM  3D images from the  hippocampal CA1 SR of 6 mo WT, Trem2  R47H KI, NL-F KI and NL-F KI; Trem2  R47H KI mice immunostained  for Bassoon  (cyan), a presynaptic  marker enriched in functional  active zones. Scale bar, 1 μm."
NCBI gene IDs:
"Trem2: 83433"
Figure legend: "G. Representative  western blots  comparing levels of cleaved  caspase-3 (17/19 kDa) and procaspase-3  (35 kDa) with respect to GAPDH  (38 kDa) loading control in synaptosomes  isolated from human  NDC, AD, NDC TREM2  and AD TREM2 brains. 1 lane represents 1 patient."
NCBI gene IDs:
"TREM2: 54209"
Figure legend: "H. Western blot densitometry  analysis showing cleaved caspase-3 levels (left graph) and the ratio of cleaved caspase-3/procaspase-3 (right graph) in synaptosomes  (SN) isolated from human  NDC, AD, TREM2  NDC and TREM2  variants. N= 9 NDC, 11 AD, 5 TREM2  NDC, 10 TREM2 AD  cases. Data information: Data shown as mean ± SEM. The top and the bottom of the box plot (H) represent the 75th and 25th percentiles, respectively, and the line represents the median. The whiskers represent the highest and lowest values that are not outliers. One-way (H) ANOVA followed by Bonferroni's post-hoc test. P-values shown as ns P&gt;0.05; *P&lt;0.05; **P&lt;0.01, ****P&lt;0.0001."
NCBI gene IDs:
"TREM2: 54209"
Figure legend: "(C) Quantitative RT-PCR analysis of mRNA encoding CD1d in freshly isolated ILC3s (from mLN of CD1d-deficient mice and mLN and spleen of WT mice), B cells and DCs (n=3). Results are normalized to those of GAPDH; RE=relative expression. **p&lt; 0.01 (two-tailed unpaired t-test)."
NCBI gene IDs:
"GAPDH: 
CD1d: 12480///12479"
Figure legend: "(D-F) Sort-purified ILC3s were preincubated with αGalCer (ILC3(αGal)) or PBS (ILC3(cont)) and adoptively transferred into WT recipients. (E) Fold change in mRNA expression for the indicated cytokines in iNKT cells sort-purified from spleen, mLN or SI-LP of mice injected with αGalCer-loaded ILC3s. Gene expression was measured by qPCR, normalized to GAPDH and presented as expression relative to iNKT cells sort-purified from mice injected with control ILC3s (n=3-5)."
NCBI gene IDs:
"GAPDH: "
Figure legend: "(A-B) Flow-cytometry plots showing gating strategy (A) and percentage of ILC populations (from Lin-CD45+CD127+cells, B) in mLN from WT (n=4) and CD1d-deficient (n=3) mice."
NCBI gene IDs:
"CD1d: 12480///12479"
Figure legend: "(C) Fold change in mRNA expression for the indicated cytokines in ILC3s after antibody-mediated CD1d crosslink (n=3). Gene expression was measured by qPCR and normalized to GAPDH and to the mRNA expression levels in control ILC3s. *p&lt;0.05 two-tailed unpaired t-test."
NCBI gene IDs:
"GAPDH: "
Figure legend: "(D) Fold change in IL22 mRNA expression in ILC3s after antibody-mediated CD1d crosslink and/or IL-23 stimulation (n=4). Gene expression was measured by qPCR and normalized to GAPDH and to the mRNA expression levels in control ILC3s. **p&lt;0.01 two-tailed unpaired t-test."
NCBI gene IDs:
"GAPDH: 
IL22: 50929"
Figure legend: "(I-J) Fold change in mRNA expression for the indicated cytokines in ILC3s sort-purified from spleen (I) or SI-LP (J) 6 h after intravenous (I) or oral (J) αGalcer administration. Gene expression was measured by qPCR and normalized to GAPDH and to the expression levels in ILC3s sort-purified from PBS-injected mice (n=3). *p&lt;0.05, **p&lt;0.01 two-tailed unpaired t-test."
NCBI gene IDs:
"GAPDH: "
Figure legend: "A Immunoblot of CCNL1 and FBXW7 expression in HPAF-II wild-type and FBXW7-/- cells, representative blot of 3 independent replicates."
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "B Immunoblot of CCNL1 expression following a cycloheximide chase in HPAF-II wild-type and FBXW7-/- cells , representative blot of 3 independent replicates. C Quantification of cycloheximide chase in B, mean ± SEM of three independent replicates."
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "D Immunoblot of CCNL1 expression in HPAF-II wild-type and dN-Cul1 expressing cells, representative blot of 3 independent replicates."
NCBI gene IDs:
"Cul1: 8454"
Figure legend: "G Immunoblot of lysates following cycloheximide treatment of HEK293T cells expressing wild-type or degron-mutated CCNL1, representative blot of 3 independent replicates. H Quantification of cycloheximide chase in G, mean ± SEM of three independent replicates."
NCBI gene IDs:
"CCNL1: 57018"
Figure legend: "A Immunoblot of immunoprecipitation of FLAG-FBXW7 overexpressed in HEK293T cells, detecting endogenous CCNL1. Representative of three independent replicates."
NCBI gene IDs:
"FLAG: 
FBXW7: 55294"
Figure legend: "B Immunoblot of immunoprecipitation of FLAG-CCNL1 overexpressed in HEK293T cells, detecting endogenous FBXW7. Representative of three independent replicates."
NCBI gene IDs:
"FLAG: 
CCNL1: 57018"
Figure legend: "D Immunoblot of cellular ubiquitination assay demonstrating the requirement of FBXW7 for ubiquitination of CCNL1. Representative of three independent replicates."
NCBI gene IDs:
"CCNL1: 57018
FBXW7: 55294"