EMBO/SourceData huggingface dataset:
Figure legend: "Drp1 knockdown cells were treated with DMSO (control) or with actinomycin D for 14 h to induce apoptosis and were decorated with antisera against Bax, cytochrome c, and Mic27 or Mic60 (see Appendix Figure S4). Three-color images were taken, whereby Mic27 or Mic60 were recorded in the STED mode. Relying on the Bax and the cytochrome c signals, we discriminated between healthy and apoptotic mitochondria before and after cytochrome c release. The distributions of Mic27 or Mic60 were analyzed by determining the normalized variance of the fluorescence intensity, which is a sensitive measure for the distribution of the labelled protein. The numbers within the columns represent the numbers of images analyzed and the error bars represent the standard error of the mean."
NCBI gene IDs:
"Drp1: 10059"
Figure legend: "D Relative change in the growth rates of E. coli K-12 BW25113 Δpgi and ΔpfkA strains in the presence of 10 mM acetate. Mean values ± standard deviations (error bars) were estimated from three independent biological replicates."
NCBI gene IDs:
"pfkA: 948412
pgi: 948535"
Figure legend: "Relative change in the growth rates of wild-type, Δpta, and Δacs E. coli K-12 BW25113 strains in the presence of 10 mM acetate at low glycolytic flux (100 mM αMG). Mean values and standard deviations (error bars) were estimated from n independent biological replicates, as indicated on the figure."
NCBI gene IDs:
"acs: 948572
pta: 946778"
Figure legend: "A,B Impact of the presence of 10 mM acetate on the growth rate of wild-type, Δpta, and Δacs E. coli K-12 BW25113 strains grown on (A) 30 mM glycerol and (B) 15 mM galactose. Mean values and standard deviations (error bars) were estimated from three independent biological replicates."
NCBI gene IDs:
"acs: 948572
pta: 946778"
Figure legend: "Immunoblot analysis of proteins biotinylated  and isolated as in (A). Parental HeLa-T-REx cells or cells expressing H3.1-BirA*-HA and treated with biotin for indicated times."
NCBI gene IDs:
"HA: 
BirA: 948469
H3.1: 8350"
Figure legend: "Immunoblot  analysis of TAP-H3.1 or -CENP-A pulldown of UBR7-LAP (schematic, upper).  293T cells were co-transfected with UBR7-LAP and TAP-tagged constructs, which were purified on IgG-conjugated sepharose beads."
NCBI gene IDs:
"LAP: 
TAP: 
UBR7: 55148"
Figure legend: "Representative images of HeLa  cells transfected with UBR7-FL. DNA is visualized via  DAPI staining shown in magenta and immunofluorescence for 3xFLAG is shown in green. The area within yellow dotted box in (E) is magnified 4x in (E'). Scale bar = 5 μm.   Quantification of the nuclear anti-FLAG immunofluorescence  intensity in (E). Error bars represent mean +/- standard deviation. One way ANOVA was used to calculate p-values (italics) associated with differences between treatments. Experiment in (E-F) was performed twice.  "
NCBI gene IDs:
"FL: 
UBR7: 55148"
Figure legend: "Immunoblot  analysis of anti-H3.1-HA pulldown of full-length UBR7-LAP and UBR7PHD fragment (residues 117-215) (schematic, upper). 293T cells were transfected as in (A). Values below pulldown  represent H3.1-HA-normalized GFP-UBR7 immunoblot signal relative to UBR7-FL. Data were acquired from a continuous membrane, and the image is cropped to omit irrelevant samples. Numbers below  blot  indicate HA-normalized anti-GFP immunoblot  signal relative to full length UBR7-LAP."
NCBI gene IDs:
"LAP: 
UBR7: 55148"
Figure legend: "Anti-GFP immunoblot analysis of streptavidin pulldown of the indicated biotin-conjugated H3  peptides following incubation with 293T-GFP-UBR7 cell lysate after mutation of residues highlighted in (C)."
NCBI gene IDs:
"GFP: 
UBR7: 55148"
Figure legend: "Immunoblot  analysis for total and modified histones in anti-FLAG pulldown of constructs from soluble cellular fractions demonstrates that UBR7 UBR box and PHD are necessary for binding to post-nucleosomal H3/H4."
NCBI gene IDs:
"UBR7: 55148"
Figure legend: "Representative images of HeLa cells transfected with sNASP-FL. DNA is visualized via DAPI staining shown in magenta and immunofluorescence for 3xFLAG is shown in green. Scale bar = 5 μm.   Quantification of the nuclear anti-FLAG immunofluorescence intensity in (G) demonstrating that  sNASP-FL is highly susceptible to pre-extraction with Triton X-100. One way ANOVA was used to determine p-values (italics) associated with differences between treatments. Error bars represent mean +/- standard deviation. Experiment in (G-H) was performed twice.  "
NCBI gene IDs:
"FL: 
sNASP: 4678"
Figure legend: "Immunoblot analysis of H3, UBR7, and GAPDH in the non-nucleosomal fraction of parental and 293T-UBR7KO cells.   Quantification of anti-H3 immunoblot represented in (A). Immunoblot signals were normalized to anti-GAPDH signal. Error bars represent mean +/- standard deviation. A two-tailed one-sample t-test was used to calculate p-value (italics) associated with group mean difference from a hypothetical value of 1.0 (represented by dashed line). Experiment in (A-B) was performed in three times.  "
NCBI gene IDs:
"UBR7: 55148"
Figure legend: "Immunoblot analysis of anti-FLAG pulldown of sNASP-FL in parental or  293T-UBR7KO cells. 3xFLAG tagged sNASP-FL was immunoprecipitated  from non-nucleosomal fraction and eluted with FLAG peptide. Experiment was performed three times.   Quantification of anti-FLAG (sNASP-FL), and anti-histone  immunoblots represented in (C). Immunoblot  signals were normalized to anti-FLAG signal. Error bars represent mean +/- standard deviation. A two-tailed one sample t-test was used to calculate p-values (italics) associated with group means difference from a hypothetical value of 1.0 (represented by dashed line). Experiments were performed two (sNASP,  H4) to three (all other targets) times.  "
NCBI gene IDs:
"UBR7: 55148"
Figure legend: "Immunoblot for LAMP2A in parental 293T cells or cells expressing   UBR7-FL (left) or  sNASP-FL (right) following treatment with siCTRL, siLAMP2A, or MG-132."
NCBI gene IDs:
"FL: 
LAMP2A: 3920
sNASP: 4678
UBR7: 55148"
Figure legend: "Heatmaps of ATAC-seq, anti-GFP (GFP-UBR7) ChIP-seq, and anti-H3K4me3 CUT&amp;RUN in parental and  UBR7KO cells. Plots are centered on TSS that­­­­­ show changes in ATAC-seq signal in  UBR7KO versus parental cells. Sites are clustered by unsupervised k-means analysis. Cluster color key is defined by cluster labels."
NCBI gene IDs:
"UBR7: 55148"
Figure legend: "A) Representative images of TgN3R182C150, sham- and NOTCH3  EGF1-5-immunized mice  at 7 months of age  and TgN3R182C150 at 18 months of age. Representative images show brain arteries  of TgN3R182C150 (7 and 18 months), sham and NOTCH3  EGF1-5-immunized mice stained  with a monoclonal antibody against NOTCH3  ECD (1E4, red) and an α-SMA  antibody (green). Scale bar =20µm. B) Quantification of NOTCH3  ECD deposits (numbers per 1,000 μm2) and NOTCH3  ECD stained  area and average size per vessel  revealed no decrease in NOTCH3  ECD deposition in brain arteries  between NOTCH3  EGF1-5 -immunized (n=10), sham (n=8) and  non-vaccinated  (n=6) TgN3R182C150 mice  at 7 months of age. NOTCH3  ECD deposits (numbers per 1,000 μm2) and NOTCH3  ECD stained  area and average size per vessel  increases significantly in the TgN3R182C150 mice  at 18 months (n=3) of age versus NOTCH3  EGF1-5 -immunized (n=10), sham (n=8) and non-vaccinated (n=6) TgN3R182C150 mice  at 7 months of age. Statistical significance was assessed using a Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparisons. (NOTCH3  ECD deposits (% of vessel  area): 7 m.o. vs. Sham, ns P=0.9424; 7 m.o. vs. Vaccinated, ns P=0.9809; 7 m.o. vs. 18 m.o.**P=0.0046; Sham vs. Vaccinated, ns P=0.9999; Sham vs. 18 m.o. **P=0.0032; Vaccinated vs. 18 m.o. **P=0.0032). NOTCH3  ECD deposits (number/1000 um2): 7 m.o. vs. Sham, ns P=0.7877; 7 m.o. vs. Vaccinated, ns P=0.8358; 7 m.o. vs. 18 m.o.**P=0.0069; Sham vs. Vaccinated, ns P=0.9995; Sham vs. 18 m.o. **P=0.0044; Vaccinated  vs. 18 m.o. *P=0.0104). NOTCH3  ECD deposits size: 7 m.o. vs. Sham, ns P=0.1169; 7 m.o. vs. Vaccinated, ns P&gt;0.9999; 7 m.o. vs. 18 m.o.***P=0.0009; Sham vs. Vaccinated, ns P=0.0577; Sham vs. 18 m.o. **P=0.007; Vaccinated  vs. 18 m.o. ****P&lt;0.0001, ns=non-significant). Error bars indicate standard error of the mean (SEM)."
NCBI gene IDs:
"N3: 4854"
Figure legend: "A) Representative images of TgN3R182C150, sham- and NOTCH3  EGF1-5-immunized mice  at 3, 7 and 18 months of age. Representative images show brain arteries  and capillaries  of TgN3R182C150, sham and NOTCH3  EGF1-5-immunized mice stained  with a monoclonal antibody against NOTCH3  ECD (1E4, red) and an anti-perlecan  antibody (green). Scale bar =20µm. B) Quantification of NOTCH3  ECD deposits (numbers per 1,000 μm2) and NOTCH3-ECD stained  area and average size per vessel   revealed a significant increase in NOTCH3  ECD deposition in brain arteries  and capillaries  between non-vaccinated  3 months old TgN3R182C150 (n=3) and 7 months old TgN3R182C150 (n=6) mice  and 18 months old TgN3R182C150 (n=3). Quantification of NOTCH3-ECD deposits (numbers per 1,000 μm2) and NOTCH3-ECD stained  area and average size per vessel  revealed a significant decrease in NOTCH3-ECD deposition in brain arteries  and capillaries  between NOTCH3  EGF1-5 -immunized (n=11), sham (n=8) and non-vaccinated  TgN3R182C150 (n=6) mice. Statistical significance was assessed using a Brown-Forsythe and Welch ANOVA tests followed by Dunnett's T3 multiple comparisons. (NOTCH3  ECD deposits (% of vessel  area): 3 m.o. vs. 18 m.o. **P=0.0029; 3 m.o. vs. 7 m.o. ***P=0.0004; 7 m.o. vs. Sham, ns P=0.7326; 7 m.o. vs. Vaccinated, **P=0.003; Sham vs. Vaccinated, ****P&lt;0.0001). NOTCH3  ECD deposits (number/1000 um2): 3 m.o. vs. 18 m.o. **P=0.0038; 3 m.o. vs. 7 m.o. ***P=0.0002; 7 m.o. vs. Sham, ns P=0.9913; 7 m.o. vs. Vaccinated, *P=0.021; Sham vs. Vaccinated, ***P=0.001). NOTCH3  ECD deposits size: 3 m.o. vs. 18 m.o. *P=0.0215; 3 m.o. vs. 7 m.o. **P=0.0029; 7 m.o. vs. Sham, ns P=0.3622; 7 m.o. vs. Vaccinated, *P=0.0428; Sham vs. Vaccinated, **P=0.0038, ns= non-significant). Dotted lines indicate quartiles and dashed thicker lines are the median."
NCBI gene IDs:
"N3: 4854"
Figure legend: "A) NOTCH3  ECD was detected in the whole blood serum  of the non-treated TgN3R182C150 mice  at three months (n=9) of age and further increased at seven months (n=6) of age. Serum from Notch3  -/- and C57Bl6/J WT mice  were included as negative controls. Statistical analysis was performed using unpaired student t test with Welch's correction. (3 m.o. vs 7 m.o. ***P=0.0005). Error bars indicate standard error of the mean (SEM)."
NCBI gene IDs:
"N3: 4854
Notch3: 18131"
Figure legend: "B) NOTCH3  ECD in the TgN3R182C150 mice  was significantly reduced in the vaccinated  (n=10) TgN3R182C150 mice  vs sham (n=9). Statistical analysis was performed using unpaired student t test with Welch's correction. (Sham vs Vaccinated  *P=0.0196). Error bars indicate standard error of the mean (SEM)."
NCBI gene IDs:
"N3: 4854"
Figure legend: "A) Representative images show microglia stained  with anti-CD68  antibody (red) and Iba1  antibody (green). Scale bar=20µm. B) Quantification of CD68-stained  area revealed a significant increase in the % of microglia  and microglia  area between N3  EGF1-5-immunized (n=6), sham (n=4) and non-vaccinated TgN3R182C150 (n=5) mice. Statistical significance was assessed using an ordinary one-way ANOVA followed by Tukey's multiple comparisons test. (% microglia  with CD68 staining: 7 m.o. vs. Sham ns P=0.7393; Sham vs. Vaccinated  **P=0.0087. CD68 staining  (% of microglia  area): 7 m.o. vs. Sham ns P=0.9131; Sham vs. Vaccinated  *P=0.0157, ns=non-significant). Error bars indicate standard error of the mean (SEM)."
NCBI gene IDs:
"N3: 4854"
Figure legend: "C) Representative images of TgN3R182C150, sham- and NOTCH3  EGF1-5-immunized mice  at 7 months of age stained  with a monoclonal antibody against NOTCH3  ECD (1E4, red) and an antibody against microglia  (Iba1, green). Scale bar=20µm. D) Quantification of NOTCH3  ECD deposits (numbers per 1,000 μm2) and NOTCH3  ECD stained  area and average size per microglia  revealed no alterations between the NOTCH3  EGF1-5-immunized (n=11), sham (n=8) and non-vaccinated  TgN3R182C150 (n=6) mice  at 7 months of age. Statistical significance was assessed using an ordinary one-way ANOVA followed by Tukey's multiple comparisons test. (% microglia with NOTCH3  ECD deposits: 7 m.o. vs. Sham ns P=0.7997; Sham vs. Vaccinated  ns P=0.0526. NOTCH3  ECD deposits (% of microglia  area): 7 m.o. vs. Sham ns P=0.5362; Sham vs. Vaccinated  ns P=0.8059. NOTCH3  ECD deposits (number/1000 um2): 7 m.o. vs. Sham ns P=0.8392; Sham vs. Vaccinated  ns P=0.5777. NOTCH3  ECD deposits size: 7 m.o. vs. Sham ns P=0.6664; Sham vs. Vaccinated  ns P=0.8787, ns=non-significant). Error bars indicate standard error of the mean (SEM)."
NCBI gene IDs:
"N3: 4854"
Figure legend: "Immunoblots and qRT-PCRs showing that expression levels of miR-203, miR-200, and E-cadherin are increased after ZEB1 knockdown in Panc1, MDA-MB-231. Vice versa, the drug-resistant clones of BxPC3, H358, and DU-145 show increased expression of ZEB1 and decreased expression of the miRNAs and E-cadherin. n = 3, mean ± SEM, except for H358 (data from microarray). Unpaired Student's t-test."
NCBI gene IDs:
"miR-200: 406985///406984///406983
miR-203: 406986
ZEB1: 6935"
Figure legend: "Lentiviral overexpression of miR-200c and miR-203 in Panc1 and hPaca1 induces sensitivity to gemcitabine treatment as measured by MTT assay. For the changes in EC50 values, see Table1. n = 3, mean ± SEM, Dunnett's multiple comparisons test (P-values in the graphs are *P = 0.01-0.05, **P = 0.001-0.01, ***P &lt; 0.001, and ****P &lt; 0.0001; for exact P-values, see Supplementary Table S4)."
NCBI gene IDs:
"miR-200c: 406985
miR-203: 406986"
Figure legend: "Overexpression of miR-203 decreases expression of the anti-apoptotic factor survivin and sensitizes to gemcitabine-triggered apoptosis as evaluated by cleaved caspase-3 in Western blot and immunofluorescence. Panc1 and hPaca1 were treated with 50 and 5 nM gemcitabine, respectively, for 48 h. Scale bar 20 μm."
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "MTT assay showing increase in gemcitabine resistance after inhibition of endogenous miRNAs in hPaca2 by specific antagomirs against miR-203 or all miR-200 members. For the changes in EC80 values, see Table1. n = 3, mean ± SEM, Dunnett's multiple comparisons test (P-values in the graphs are *P = 0.01-0.05, **P = 0.001-0.01, ***P &lt; 0.001, and ****P &lt; 0.0001; for exact P-values, see Supplementary Table S4)."
NCBI gene IDs:
"miR-200: 406985///406984///406983
miR-203: 406986"
Figure legend: "Overexpression of miR-203 shows reduced numbers of the CD24/CD44 double-positive cancer stem cell population as determined by FACS analysis. The arrow indicates the reduction in the CD24 high subpopulation and reduction in CD133 by miR-203 overexpression in hPaca1 cells."
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "Cancer stem cell sphere assay showing reduced sphere-forming capacity of Panc1 and hPaca1 in miR-203 overexpression cells. Colonies with a diameter greater than 75 μM for Panc1 and greater 30 μM for hPaca1 cells were counted as spheres. n = 3, mean ± SEM, Mann-Whitney U-test."
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "B, C Histone marks were analyzed using ChIP coupled to qRT-PCR for Panc1 control versus shZEB, MDA-MB-231 control versus shZEB (B), and BxPC3 control versus gemcitabine resistant (gr) (C). In MDA-MB-231 and Panc1, the active histone marks H3K4me3, H3ac, H4ac, and H3K9ac were enriched. Vice versa, in the drug-resistant clones of BxPC3, the active marks were reduced in the CpG islands. The repressive histone mark H3K27me3 was not detectable in the miR-200 loci, but in the loci of miR-203 and E-cadherin in Panc1 and MDA-MB-231. DNA methylation status was determined by bisulfite sequencing. Depletion of ZEB1 in MDA-MB-231 resulted in almost complete demethylation, whereas the selection of drug-resistant, ZEB1-expressing clones in BxPC3 induced complete methylation. n = 2 (Panc1) or 3 (MDA-MB-231 and BxPC3), mean ± SEM; unpaired Student's t-test."
NCBI gene IDs:
"E-cadherin: 999
miR-200: 406983///406985///406984
miR-203: 406986
ZEB: 6935
ZEB1: 6935"
Figure legend: "Heat map showing the relative expression levels after drug treatment for 48 h in Panc1. Values measured by qRT-PCR were depicted with the software GENE-E. Only mocetinostat upregulated the miRNAs and downregulated ZEB1."
NCBI gene IDs:
"ZEB1: 6935"
Figure legend: "Relative expression of indicated genes in Panc1 measured by qRT-PCR after treatment with different HDAC inhibitors. Note the downregulation of ZEB1 and upregulation of miR-203, miR-200, and E-cadherin by mocetinostat. n = 3, mean ± SEM; unpaired Student's t-test. For significance, see Supplementary Table S1."
NCBI gene IDs:
"E-cadherin: 999
miR-200: 406983///406985///406984
miR-203: 406986
ZEB1: 6935"
Figure legend: "Chromatin immunoprecipitation analysis validated mocetinostat-induced (1 μM, 48 h) enrichment of the active histone marks H3ac, H4ac, H3K9ac, and H3K4me3 at ZEB1 target gene loci in Panc1. n = 3, mean ± SEM; unpaired Student's t-test."
NCBI gene IDs:
"ZEB1: 6935"
Figure legend: "Mocetinostat treatment reduced expression of the anti-apoptotic miR-203 target survivin and sphere-forming capacity in Panc1 when pre-treated with mocetinostat for 48 h. n = 3, mean ± SEM; Mann-Whitney U-test."
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "MTT assays comparing the effects of docetaxel and mocetinostat in the prostate cancer cell line DU-145 and the docetaxel-resistant subclone DU-145 DR (left). Mocetinostat treatment of DU-145 DR downregulates ZEB1, upregulates E-cadherin, miR-200, and miR-203 expression. For relative miRNA expression, the expression levels in original DU-145 were set to 1 (middle panels, the immunoblot panel derives from the same experiment shown in Fig1A). Mocetinostat sensitizes DU-145 DR to docetaxel (right). For calculation of the CI and synergy between the drugs, see Table2. n = 3, mean ± SEM, Dunnett's multiple comparisons test (P-values in the graphs are *P = 0.01-0.05; for exact P-values, see Supplementary Table S4)."
NCBI gene IDs:
"miR-200: 406983///406985///406984
miR-203: 406986"
Figure legend: "Representative pictures of in situ hybridization for miR-203 and control probe showing gain of miR-203 and associated loss of ZEB1 detected by immunohistochemistry in serial sections of mocetinostat-treated xenograft tumors. Scale bar 40 μm, inserts for higher magnifications 5 μm. Squares indicate magnified regions."
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "Schematic outline and results for xenografts of ex vivo treated Panc1 in Foxn1 nude mice. Panc1 cells were pre-treated with mocetinostat (1 μM) and/or gemcitabine (50 nM) for 48 h, followed by a 7-day recovery period before being injected subcutaneously (left). Equal numbers of viable cells were injected in 75 μl volume. At day 9 after injection, tumor growth was detectable in all groups (lower right). To better visualize and compare tumor growth, the tumor volume at day 9 was set to 1 and the increasing slope of the tumor volume to day 37 is depicted (upper right). The individual absolute tumor volumes on day 37 (lower left) and the group medians of the absolute tumor volumes over time (lower right) are shown. For cells pre-treated with the combination of mocetinostat and gemcitabine, tumor growth was arrested. n = 4 for each treatment group; nonparametric Mann-Whitney U-test."
NCBI gene IDs:
"Foxn1: 15218"
Figure legend: "A, B Relative expression levels of miR-203 and miR-200c in pancreatic adenocarcinomas. (A) In normal versus tumor tissue of the same case (n = 6 cases), (B) in the non-recurrence (n = 10 cases) versus the recurrence group (n = 11 cases). The mean value of the lower group in each figure was set to 1. Nonparametric Mann-Whitney U-test."
NCBI gene IDs:
"miR-200c: 406985
miR-203: 406986"
Figure legend: "B Box plots of the Em/Ad expression ratios of GATA6 (left) and NKX2-1 (right) in FFPE lung tissue sections from controls (Ctrl, n=61) or lung cancer (LC, n=51) patients. Isoform-specific expression of the indicated genes was analyzed by qRT-PCR after total RNA isolation from tissue samples. Each point represents one sample."
NCBI gene IDs:
"GATA6: 2627
NKX2-1: 7080"
Figure legend: "C Box plots of Em/Ad of GATA6 (top) or NKX2-1 (bottom) show that high Em/Ad ratios in LC samples are maintained among ethnic groups (left) and gender (right). GER, samples collected in Germany; MEX, samples collected in Mexico."
NCBI gene IDs:
"GATA6: 2627
NKX2-1: 7080"
Figure legend: "D Box plots of Em/Ad of GATA6 (left) or NKX2-1 (right) in FFPE lung tissue sections from controls or LC patients. Samples were staged according to the TNM Classification (UICC, 7th edition)."
NCBI gene IDs:
"GATA6: 2627
NKX2-1: 7080"
Figure legend: "A Box plots of the Em/Ad expression ratios of GATA6 (left) and NKX2-1 (right) in EBCs from controls (Ctrl, n=65) or lung cancer (LC, n=48) patients (training set). Each point represents one sample."
NCBI gene IDs:
"GATA6: 2627
NKX2-1: 7080"
Figure legend: "Cells were grown in MHB-ca medium without or with 20 mM glucose as indicated. (B) PndhC and PndhF YFP reporter constructs were used for following gene expression and data was normalized by growth. Data are expressed as average ± SEM of three independent experiments. *** Denotes p &lt; 0.001 compared to ndhC expression in WT strain grown in MHB-ca medium via two-way ANOVA with Tukey's posttest."
NCBI gene IDs:
"YFP: 
ndhF: 3919222
ndhC: 3919225"
Figure legend: "(A) PndhC and PndhF YFP reporter constructs were used to follow gene expression under the biofilm growth conditions. Data collected after 24 h of static incubation was normalized by optical density at 600 nm. Data are expressed as average ± SEM of three independent experiments. No significant differences were found when ndhC expression in WT strain was compared via one-way ANOVA with Dunnett's posttest."
NCBI gene IDs:
"YFP: 
ndhF: 3919222
ndhC: 3919225"
Figure legend: "(E) Hemolytic activity of Newman ∆ndhC strain overexpressing the N. gonorrhea acyl-ACP synthetase and grown aerobically for 20 h in MHB-ca medium. Data are expressed as average ± SD of four independent experiments. ** Denotes p &lt; 0.005 compared to ∆ndhC strain with the empty plasmid via Student's t-test."
NCBI gene IDs:
"acyl-ACP synthetase: 
ndhC: 3919225"
Figure legend: "(F) Biofilm formation of Newman ∆ndhC strain overexpressing the N. gonorrhea acyl-ACP synthetase and grown under biofilm producing conditions. Data are expressed as average ± SD of three independent experiments performed by octuplicates. *** Denotes p &lt; 0.001 compared to ∆ndhC strain with the empty plasmid via Student's t-test."
NCBI gene IDs:
"acyl-ACP synthetase: 
ndhC: 3919225"
Figure legend: "C The peritoneal recruitment of classical monocytes after IP injection of PBS (n = 6) or TG in C57BL/6J WT (dark green, n = 10 mice) and Gal-3-/- (black, n = 5 mice) mice was assessed after 18 h. Where indicated, the mice received an IP injection of CXCR4 antagonist AMD 3465 12 hours prior to the experiment."
NCBI gene IDs:
"Gal-3: 16854"
Figure legend: "Filter Trap with 5-fold serial dilution and quantification of crude protein extract from SERF2 CRISPR  deletion mutant cells transiently transfected with HA-polyQ74 and either wild-type SERF2, empty vector or SERF2  charge mutant (I), Western blots for HA-Q74, SERF2  and Tubulin  expression were included as controls For all Filter Trap assays, the depicted blots  are from one representative experiment of four biological replicates. The average of four biological replicates with each three or four technical replicates is represented in the graphs. Data are represented as mean ± SD, significance was calculated using a one-way ANOVA, followed by a post-hoc Bonferroni multiple comparisons test. (I) p&lt;0.0001, **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"CRISPR: 
SERF2: 10169"