EMBO/SourceData huggingface dataset:
Figure legend: "A, TRIM33  mRNA expression  levels were analyzed in normal and tumor samples from multiple publicly available patient  cohorts. A two-sided Wilcoxon signed-rank test was used for the TCGA Prostate, Ding 2016, and Jing 2020 datasets, while a two-sided Wilcoxon rank-sum test was used for the other datasets. Measurements shown in box plots are the first and third quartiles and are split by the medians, whiskers extending a 1.5-fold interquartile range beyond the box."
NCBI gene IDs:
"TRIM33: 51592"
Figure legend: "(C) Confocal z-stack images showing the presence of ARSA-positive cells (green) co-stained with oligodendrocyte markers CNPase and APC (arrows). (D) Undetectable ARSA immunoreactivity in tissues of untreated animals. In all pictures nuclei are counterstained with ToPro (blue). Scale bars: A, 80 µm; B, D 60µm, C, 80µm"
NCBI gene IDs:
"ARSA: 410"
Figure legend: "Vector copy number (VCN) cartography shows integrated LV genome (assessed by qPCR) along the rostro-caudal axis (slices 1-10) in LV.hARSA-injected S1.1 and S2.2 NHP in a side-by-side comparison with ARSA mRNA expression (assessed by qPCR analyses using probe and primers annealing to sequences conserved in both the human and Macaca fascicularis and expressed as fold increase to region-matched blocks of the contralateral hemisphere). Grading of colors for VCN ranged from white (VCN&lt;0.001; corresponding to CT&gt;37) to dark orange (VCN=1-3). The highest VCN is found in close correspondence to the injection sites, as confirmed by comparison with post-surgery MR images (yellow and orange circles indicate viral suspension close to the injection sites). Grading of colors for ARSA expression ranges from white (&lt;1.5 fold the physiological level) to dark purple (100-fold the physiological level)."
NCBI gene IDs:
"ARSA: 410"
Figure legend: "(E) ARSA activity in the spinal cord of individual LV.GFP- (n=4 blocks/animal, 2-3 replicates) and LV.hARSA-injected NHPs (n=2-4 blocks/animal; n=10 and n=11 total blocks for study group 1 and study group 2, respectively). Graph on the right represent the summary of ARSA activity in each treatment group. One-way ANOVA and Dunnet's multiple comparison test. * p&lt;0.05 versus pilot group."
NCBI gene IDs:
"ARSA: 410"
Figure legend: "(F) ARSA activity in the sciatic nerve of LV.GFP- (n=2) and LV.hARSA-injected NHPs (n=3). In B-F data are expressed as floating bars (line at mean)."
NCBI gene IDs:
"ARSA: 410"
Figure legend: "(G) ARSA activity in the cerebrospinal fluid (CSF) of UT/LV.GFP (n=3) and LV.hARSA-injected NHP (n=5). Data are expressed as mean±SEM. Student t-test, *p=0.035."
NCBI gene IDs:
"ARSA: 410"
Figure legend: "(A) Vector copy number (VCN) indicating the distribution of integrated LV genome along the rostro-caudal axis (slices 7-19) in LV.hGALC-injected NHPs (JT02 and JV02) assessed by qPCR. Each dot represent the VCN measured in one block within the slice. Lower threshold (dotted line): VCN&lt;0.001, corresponding to CT&gt;37."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(B) GALC mRNA expression along the rostro-caudal axis of JT02 (slices 7-13) is expressed as fold increase to region-matched blocks of the contralateral hemisphere (dotted line, y=1). Data are expressed as floating bars (min to max, line at mean; n=4-8 blocks/slice). Arrows on x axis in A and B indicate the injection sites."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(A) Specific GALC enzymatic activity in single brain slices of WT untreated (UT; white bars, physiological levels), WT LV.hGALC-treated (JV02) and Krabbe-affected LV.hGALC-treated (JV02) animals (filled grey bars, injected hemisphere; striped grey bars, contralateral hemisphere). Two-way ANOVA followed by Bonferroni's multiple comparison tests; *p&lt;0.05, **p&lt;0.01 vs matched slices of WT UT."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(B-C) Summary of GALC activity in brain (B) samples from JT02 and JV02, as well as from WT and Krabbe UT controls. Data in A-C are represented as floating bars (min to max, line at mean; n=2-6 blocks/slice). Number of blocks analysed/animal: 16 (WT UT), 24 (JT02), 22 (JV02). GALC activity in Krabbe UT samples is &lt; 0.001 nmol/h*mg; n=3 blocks. Kruskal-Wallis test and Dunn's multiple comparison test; *p&lt;0.05, ***p&lt;0.001 vs WT UT."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(B-C) Summary of GALC activity in spinal cord (C; n=3 blocks/animal) samples from JT02 and JV02, as well as from WT and Krabbe UT controls. Data in A-C are represented as floating bars (min to max, line at mean; n=2-6 blocks/slice). Number of blocks analysed/animal: 16 (WT UT), 24 (JT02), 22 (JV02). GALC activity in Krabbe UT samples is &lt; 0.001 nmol/h*mg; n=3 blocks. Kruskal-Wallis test and Dunn's multiple comparison test; *p&lt;0.05, ***p&lt;0.001 vs WT UT."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(D) Chromatographic profile of GALC in selected brain slices and spinal cord (SC) tissue of untreated (UT) WT and LV.hGALC-treated Krabbe NHP (JT02). Extracts were run through a Sephadex S-300 gel filtration column. Fractions (0.2 ml) were assayed for enzyme activity using MUGAL substrate in the presence () or absence of 11µM AgNO3 (●)."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(E) GALC activity in the CSF of JT02 (pre- and post-treatment) and JV02 (post-treatment). Dots represent technical replicates."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "(F) Beginning at two months (m) of age, animals were evaluated monthly for motor performance using a modified Bayley's scale for infant development. For the study animals (JV02 and JT02), scores are reported as pre-surgery and post-surgery. Historical data for normal and affected animals are presented as means plus one standard deviation. Affected animals score significantly lower than normal animals at any age considered. JT02's motor scores increase over time post LV.GALC injection, being close to the mean observed in normal juveniles at 5 months. Historical data are analyzed by two-way ANOVA and Bonferroni posttests, p&lt;0.05 at 6m, p&lt;0.001 at all other ages. n=5-20 animals/group, except for normal animals at 6 months (n=1)."
NCBI gene IDs:
"GALC: 2581"
Figure legend: "F Visualization of infected region. E. coli FM15  was inoculated at the center of a semi-solid agar plate and the reporter phage M13-sfGFP was inoculated 1-cm away from the center. Fluorescence images were captured after overnight incubation using FITC  channel and an exposure time of 200 ms. Scale bar represents 1 cm. G Relative fluorescence intensity at the center and edge positions of the infection  zone as shown in (F). The relative intensity was obtained by dividing the detected values with the maximum value of green fluorescence intensity on the plate. Data represent mean values ± s.d. for three values of the center and six values of the edge from three biological replicates. Two-tailed t-test was used to compare two groups. ***, p = 0.0005."
NCBI gene IDs:
"GFP: "
Figure legend: "B Microscopic  images of E. coli FM15  cells carrying plasmids with gfp  gene downstream of the psp  promoter with or without wild-type M13 phage infection. Scale bar: 1 μm."
NCBI gene IDs:
"gfp: 
psp: "
Figure legend: "E Activity in cells of T7 RNAP  and its mutants from the SPACE  experiment in (D). Two clones of the ancestor and five clones of the mutants obtained from the evolution towards 1D8  recognition were measured by in vivo transcriptional assay  Two-tailed t-test was used to compare the two groups. ***, p=0.0001. Data represent mean values ± s.d.."
NCBI gene IDs:
"RNAP: 1261050"
Figure legend: "D Expression  activity of 10 RNAP  mutants on T7  promoter and 10 promoter variants measured by in vivo transcriptional assay. Mutants are arranged in the same order as their original target promoters listed on the left side of the heatmap. Data are normalized so that the activity of wild-type RNAP  on the T7 promoter is 100; the mean for three biologically independent replicates is shown"
NCBI gene IDs:
"RNAP: 1261050"
Figure legend: "(D) CHO  cells engineered to transiently express SNAP-mTOR, SNAP-RAPTOR, and SNAP-RICTOR  (Gautier et al., 2008) were incubated with the nuclear  export inhibitor  leptomycin B (LMB) (6.4 ng/mL) for six hours at 37°C. Cells were labeled with TMR-Star (1 μM) for 30 min, washed and then imaged. This experiment demonstrated that mTOR, RAPTOR, and RICTOR  accumulated in the nucleus  after LMB  treatment. Scale bars, 10μm."
NCBI gene IDs:
"mTOR: 
RAPTOR: 
RICTOR: 
SNAP: "
Figure legend: "(B) Cultured human keratinocytes  (YF29  passage III) treated with rapamycin  maintained a basal phenotype as shown by the expression of several basal (KRT14, BNC1) and terminal differentiation markers (SPINK5, IVL, KRT1, KRT10) using  quantitative PCR (left panel) and Western blotting (right panel). Data are presented as the mean (n = 2 biological replicates)."
NCBI gene IDs:
"BNC1: 646
IVL: 3713
KRT1: 3848
KRT10: 3858
KRT14: 3861
SPINK5: 11005"
Figure legend: "D. PCoA of the rectal bacterial 16S rRNA gene sequences based on the weighted UniFrac distance matrix."
NCBI gene IDs:
"16S rRNA: "
Figure legend: "PCoA, based on the weighted UniFrac distance matrix, of the bacterial 16S rRNA gene sequence data for the rumen, ileum, and colon, shown for the different segments of the gastrointestinal tract (B)"
NCBI gene IDs:
"16S rRNA: "
Figure legend: "C. PCoA, based on the weighted UniFrac distance matrix, of the bacterial 16S rRNA gene sequence data or the rumen, ileum, and colon, combined data (C). D. Microbial dissimilarity (calculated based on the weighted UniFrac distance matrix, 100 values in each group) between the male and CtM groups in the different intestinal compartments. The lines, boxes, and whiskers in the box plot diagrams represent the median, first and third quartiles, and min-to-max distribution of replicate values, respectively. "
NCBI gene IDs:
"16S rRNA: "
Figure legend: "C. PCoA, based on the weighted UniFrac distance matrix, of the bacterial 16S rRNA gene sequence data for the luminal contents of the ileum and colon, shown according to the different diets and antibiotic treatment."
NCBI gene IDs:
"16S rRNA: "
Figure legend: "C. PCoA, based on the weighted UniFrac distance matrix, of the bacterial 16S rRNA gene sequence data for the luminal contents of the ileum and colon."
NCBI gene IDs:
"16S rRNA: "
Figure legend: "F. PCoA, based on the weighted UniFrac distance matrix, of the bacterial 16S rRNA gene sequence data for the luminal contents of the ileum and colon are shown. The body weight gain data are presented as a percentage of the initial body weight. The fat weight data are presented as a percentage of the body weight."
NCBI gene IDs:
"16S rRNA: "
Figure legend: "(D) Similar BRET experiment showing the Rab7-pool of PI4P both in control HEK293-AT1 cells and in two clones of cells with PI4K2A deletion (#20 and #26). Means ± S.E.M. are shown from three experiments performed in triplicates."
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "(A) BRET measurement of PI4P in Rab7 compartment in parental and PI4K2A K/O cells after expression of HA-tagged PI4K2A or its kinase-dead mutant. Means ± S.E.M. are shown from three experiments performed in triplicates."
NCBI gene IDs:
"HA: 
PI4K2A: 55361"
Figure legend: "(C) Distribution of GFP-Rab7 in parental (left panels) and two clones of PI4K2A K/O cells. Representative pictures show tubulation in the K/O cells reminiscent of those seen in wild-type cells expressing the GTP-locked Rab7Q67L mutant. Scale bars are 10 µm."
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "(C) Quantification of the changes in BRET experiments. Here the BRET sensor contained the PLCδ1PH-fused to Sluc and the Venus targeted with Rab7 expressed from a single vector and it was co-transfected with the Rab7-targeted FRB (tagged with iRFP) and the FKBP12-fused PIP5Kγ in which the CFP was mutated to eliminate its fluorescence (CFP*). Recruitment of the PIP5Kγ but not its kinase-dead version caused an increase in the BRET signal indicating the increased PI(4,5)P2 in this compartment (means ± S.E.M., from three separate experiments performed in triplicates)."
NCBI gene IDs:
"CFP: 
iRFP: 
Sluc: 
Venus: 
FKBP12: 2280
PIP5Kγ: 23396
Rab7: 404007"
Figure legend: "(D) PI(4,5)P2 increases were larger when the Venus part of the BRET sensor was targeted with the GTP-locked form of Rab7 (Q67L) and was negligible with the GDP-locked form of Rab7 (N125I) in the BRET construct. Also note that the Rab7 wild-type-based BRET signal slowly returned toward the green trace consistent with Rab7 falling off from the membrane both in the BRET and the recruiting constructs (means ± S.E.M., from three separate experiments performed in triplicates)."
NCBI gene IDs:
"Venus: 
Rab7: 404007"
Figure legend: "(E) The response of PI4K2A K/O cells was significantly reduced Means ± S.E.M. are shown from three experiments performed in triplicates."
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "(A) Representative confocal images of HEK293-AT1 cells, which show that treatment with OSW1 (20 nM), a drug that inhibits the cholesterol-PI4P transport protein, OSBP, causes accumulation of PI4P in the Golgi and in the endosomes (upper panels). Note that the accumulation is much reduced in the endosome but less so in the Golgi in the PI4K2A K/O cells. Scale bars: 20 µm"
NCBI gene IDs:
"PI4K2A: 55361"
Figure legend: "(B) Quantification of these changes by BRET analysis. The PI4P-Rab7 BRET sensor construct was transfected into the respective cell lines and the cells treated with OSW1 (20 nM) for the indicated times. The BRET ratios were expressed relative to those of DMSO treated cells. Data information: Means ± S.E.M. are shown from three experiments performed in triplicates."
NCBI gene IDs:
"Rab7: 404007"
Figure legend: "(C) Detection of endosomal PI(4,5)P2 in cells pre-treated with OSW1 for 1 h in serum-free medium using confocal microscopy and the PLCδ1PH-GFP PI(4,5)P2 reporter. Angiotensin II (AngII, 100 nM) (a Gq and PLC-activating agonist) was used to liberate the PLCδ1PH-GFP PI(4,5)P2 reporter from the PM, which was necessary to detect the endosomal PI(4,5)P2. The signal is transient as the AngII receptors show desensitization. Note the reduced signal in the two PI4K2A K/O clones. Scale bars: 20 µm."
NCBI gene IDs:
"PLC: 
Gq: 2776
PI4K2A: 55361"
Figure legend: "(D) Quantification of the same changes by BRET analysis in wild-type cells using the PI(4,5)P2 sensor with Rab7-Q67L-targeting. Note the transient signal increase after AngII stimulation only in cells treated with OSW1. (E) This increase is barely detectable in the PI4K2A K/O cells. D Data information: Means ± S.E.M. are shown from three experiments performed in triplicates. The values in (D) and (E) were measured in the same 96-well plate at the same time."
NCBI gene IDs:
"PI4K2A: 55361
Rab7: 404007"
Figure legend: "(A) BRET experiment in parental HEK293-AT1 cells in OSW1-pretreated cells after RNAi mediated knock-down of PIP5Kβ or PIP5Kγ. (means ± S.E.M. from three experiments, each performed in triplicates). Note the reduced response in the PIP5Kγ knock-down cells and the slight reduction in the case of PIP5Kβ knock-down. (B) Areas below the curves calculated from the time of rapamycin addition for each of the three separate experiments shown in panel A (means ± S.E.M., n=3). One-way ANOVA with Dunnett's multiple comparisons was used for statistical analysis (* P=0.0241; **P=0.0088). ( "
NCBI gene IDs:
"PIP5Kβ: 8395
PIP5Kγ: 23396"
Figure legend: "(D) Increased appearance of GFP-LC3 positive vesicles in PI4K2A K/O cells and reduced acidification of the LC3-positive vesicles using the GFP-mCherry-LC3 reporter. Cells were transfected with the indicated constructs for one day and observed by confocal microscopy. Scale bars: 20 µm. (E) Quantification of the LC3 positive vesicles. (n = 67, 34, 26, 13, 30, 27 for the various groups, respectively from left to right). *** designates P &lt; 0.0001 using unpaired t-test. The same data set was also analyzed with the non-parametric Mann-Whitney test showing a P = 0.0004 and 0.0012 for #20 and #26 clones relative to control, respectively. (F) Co-localization analysis using the Pearson coefficient (n = 31, 21 and 14 cells analyzed for the different groups from left to right). *** designates P &lt; 0.0001 using unpaired t-test. The same data set was also analyzed with the non-parametric Mann-Whitney test showing a P value &lt; 0.0001 for both groups. "
NCBI gene IDs:
"GFP: 
LC3: 
mCherry: 
PI4K2A: 55361"
Figure legend: "(A, B) Distribution of mCherry-GFP-LC3 in HEK293-AT1 cells (without starvation) after treatment with control siRNA (A) or siRNA for PIP5Kγ (PIP5K1C). Scale bars: 20 µm. Note the massive tubulation of the LC3 compartment in some of the knocked-down cells (B, lower images, scale bar: 10 µm). (C) Comparison of Pearson coefficients from cells treated with control or PIP5Kγ RNAi. For statistical analysis the unpaired t-test was used (n = 50 and 45 cells for control a PIP5Kγ knock down groups, respectively; *** P&lt; 0.0001). (D) Representative confocal images of live HEK293-AT1 cells expressing GFP-Rab7 treated with control or PIP5Kγ RNAi. Note the tubulation in the PIP5Kγ RNAi-treated cells. Scale bars: 10 µm. "
NCBI gene IDs:
"GFP: 
LC3: 
mCherry: 
PIP5K1C: 23396
PIP5Kγ: 23396"
Figure legend: "(A) HEK293-AT1 cells were transfected with PLEKHM1-GFP-Rab7 along with CFP-FKBP-PIP5Kγ and iRFP-FRB-Rab7 constructs for one day and examined live by confocal microscopy. Representative cells show reduced PLEKHM1 localization when PIP5Kγ was recruited to the Rab7 endosomes (lower row images). (B) This response is reduced in PI4K2A K/O cells. ( Data information: Scale bars: 20 µm."
NCBI gene IDs:
"CFP: 
GFP: 
iRFP: 
FKBP: 2280
PI4K2A: 55361
PIP5Kγ: 23396
PLEKHM1: 9842
Rab7: 404007"
Figure legend: "(C) Quantification of these changes by BRET analysis where the full-length PLEKHM1 was fused to Sluc and Venus was fused to Rab7 in the BRET construct. For PIP5Kγ recruitment a mutant dark CFP(W66A)-FKBP-PIP5Kγ was used with an iRFP-FRB-Rab7. BRET values were normalized to those of DMSO treated cells. Comparisons were made for parental (blue) and PI4K2A K/O (green and orange) cells. (D) Similar BRET analysis where the PLEKHM1 was replaced by either RILP or Vps35 in the BRET construct. D Data information Means ± S.E.M. are shown from three experiments performed in triplicates."
NCBI gene IDs:
"CFP: 
iRFP: 
Sluc: 
Venus: 
FKBP: 2280
PI4K2A: 55361
PIP5Kγ: 23396
PLEKHM1: 9842
Rab7: 404007
RILP: 83547
Vps35: 55737"
Figure legend: "(A) Number of cell corpses at the L1 stage of development per 100 animals carrying the neurotoxic mec‐4(d) or deg‐3(d) alleles in SNT‐1‐deficient mutants. For mec‐4(d) mutant animals, bars denote touch receptor neuron corpses. For deg‐3(d)mutants, bars denote inner labial neuron 1 (L1) sensory neuron and PVC interneuron corpses."
NCBI gene IDs:
"deg‐3: 3565200
mec‐4: 181728
SNT‐1: 174120"
Figure legend: "(B) Expression of a full‐length MEC‐4::GFP reporter fusion under the control of the mec‐4 promoter in touch receptor neurons of wild‐type, snt‐1 and unc‐57 mutant animals. Representative photos of touch receptor neuron cell bodies are shown. Bar denotes 6 μm. The quantification (%) of GFP signal intensity from the animals examined is graphed below (n=100 neurons per assay; P0.05 compared with wild type; t‐test)."
NCBI gene IDs:
"MEC‐4: 181728
snt‐1: 174120
unc‐57: 268228"
Figure legend: "(C) Percentage of SNT‐1‐deficient animals that survive near‐lethal treatment with sodium azide. This chemical inhibits the activity of the respiratory electron transport complex IV (cytochrome C oxidase) and simulates hypoxia."
NCBI gene IDs:
"SNT‐1: 174120"
Figure legend: "(D) Number of touch receptor neuron corpses, at the L1 stage of development, per 100 animals carrying the neurotoxic mec-4(d) or deg‐3(d) alleles in UNC‐57‐deficient animals."
NCBI gene IDs:
"deg‐3: 3565200
mec-4: 181728
UNC‐57: 268228"
Figure legend: "(E) Percentage of UNC‐57‐deficient animals that survive after the hypoxia‐inducing treatment with sodium azide."
NCBI gene IDs:
"UNC‐57: 268228"
Figure legend: "(F) Number of touch receptor neuron corpses, at the L1 stage of development, per 100 animals carrying the neurotoxic mec‐4(d) or deg‐3(d) alleles together with lesions in genes encoding key proteins that participate in all four steps of clathrin‐mediated endocytosis. UNC‐11 and DPY‐23 are adaptor proteins required for the formation of clathrin‐coated pits, DYN‐1 is a GTPase necessary for fission of clathrin‐coated vesicles and UNC‐26 participates in the uncoating of vesicles. Error bars denote s.e.m. values (n&gt;250 for all populations examined; P0.001, compared with wild‐type animals, unpaired t‐test)."
NCBI gene IDs:
"deg‐3: 3565200
DPY‐23: 180713
DYN‐1: 181644
mec‐4: 181728
UNC‐11: 171952
UNC‐26: 178284"
Figure legend: "(A) Confocal images of touch receptor neurons expressing a pmec‐17APS‐2GFP reporter transgene. During early necrosis, the number of APS‐2::GFP puncta, corresponding to clathrin‐coated pits and vesicles, in touch receptor neurons ofmec‐4(d) animals does not change significantly, whereas at later stages it decreases. A similar decrease is also observed in unc‐57(e1190) mutants, defective for clathrin‐mediated endocytosis."
NCBI gene IDs:
"APS‐2: 184091
mec‐4: 181728
unc‐57: 268228"
Figure legend: "(B) Confocal images of touch receptor neurons expressing a pmec‐7GFP::RAB‐5 reporter transgene. The number of fluorescent puncta that correspond to early endosomes in touch receptor neurons of mec‐4(d) animals significantly increases during early necrosis, whereas it declines later."
NCBI gene IDs:
"mec‐4: 181728
RAB‐5: 172755"
Figure legend: "(C) Confocal images of touch receptor neurons expressing a pmec‐7GFP::RAB‐11 reporter transgene. The number of GFP::RAB‐11 puncta that correspond to recycling endosomes in touch receptor neurons of mec‐4(d) animals increases early during cell death, while it declines as degeneration proceeds. Both early and recycling endosomes tend to accumulate around a swollen structure that is probably the nucleus. Bars denote 4 μm. Error bars denote s.e.m. values (n&gt;250 for all populations examined; P0.001, compared with control animals, unpaired t‐test)."
NCBI gene IDs:
"mec‐4: 181728
RAB‐11: 4363014///171601"
Figure legend: "Endocytosis functions together with lysosomal proteolysis to facilitate necrotic cell death. (A) Depletion of synaptotagmin (SNT‐1) or endophilin (UNC‐57) does not further suppress MEC‐4(d)‐induced necrosis in cad‐1 mutants with reduced cathepsin activity or with V‐ATPase dysfunction (B, C)."
NCBI gene IDs:
"cad‐1: 
MEC‐4: 181728
SNT‐1: 174120
UNC‐57: 268228"
Figure legend: "(A) Synaptotagmin (SNT‐1) deficiency further suppresses MEC‐4(d)‐induced necrosis in animals with impaired autophagy."
NCBI gene IDs:
"MEC‐4: 181728
SNT‐1: 174120"
Figure legend: "(B) Endophilin dysfunction enhances suppression of necrosis in mec‐4(d);lgg‐1(RNAi) animals, where autophagy is impaired. Error bars denote s.e.m. values (n&gt;250 for all populations examined; P0.01, compared with single mutant control animals, unpaired t‐test)."
NCBI gene IDs:
"lgg‐1: 174050
mec‐4: 181728"
Figure legend: "(A) Depletion of the kinesin 1 heavy chain UNC‐116 suppresses necrosis induced by both mec‐4(d) and deg‐3(d) toxic alleles."
NCBI gene IDs:
"deg‐3: 3565200
mec‐4: 181728
UNC‐116: 176179"
Figure legend: "(B) Expression of a full‐length MEC‐4::GFP reporter fusion under the control of the mec‐4 promoter in touch receptor neurons of wild‐type, unc‐116 and unc‐104 mutant animals. Representative photos of touch receptor neuron cell bodies are shown. Bar denotes 6 μm. The quantification (%) of GFP signal intensity from the animals examined is graphed below (n=100 neurons per assay; P0.05 compared with wild type; t‐test)."
NCBI gene IDs:
"mec‐4: 181728
unc‐104: 174144
unc‐116: 176179"
Figure legend: "(C) Percentage of unc‐116 animals that survive after treatment with sodium azide."
NCBI gene IDs:
"unc‐116: 176179"
Figure legend: "(D) Depletion of the monomeric kinesin UNC‐104 protects neurons against MEC‐4(d)‐induced degeneration."
NCBI gene IDs:
"MEC‐4: 181728
UNC‐104: 174144"
Figure legend: "(E) unc‐104 mutant animals are more resistant to hypoxic death induced by sodium azide."
NCBI gene IDs:
"unc‐104: 174144"