EMBO/SourceData huggingface dataset:
Figure legend: "(C) Northern (top panel) and western (middle, bottom panels) blot analyses of total RNA and protein from SOD1G85R strains, with (M1/M1) or without (+/+) suppressor mutations, demonstrating that the levels of SOD1G85R mRNA and total protein are not changed by the suppressor mutation (M1/M1). The western blot lanes are from the same gel and exposure."
NCBI gene IDs:
"SOD1: 6647"
Figure legend: "(F) Locomotor behavior, measured by thrashing rates, of the C. elegans carrying the SOD1G85R transgene on the normal background (WT), with the null mutation of either ufd-2(tm1380) or spr-5(by134), the double mutation ufd-2(tm1380);spr-5(by134), or the M1 suppressor ufd-2(W824X);spr-5(R646Q) (n = 16)."
NCBI gene IDs:
"SOD1: 6647
spr-5: 173214
ufd-2: 174295"
Figure legend: "(G) Western blotting of the supernatant (S) and pellet (P) protein fractions from the C. elegans carrying the SOD1G85R transgene with the double mutation ufd-2(tm1380);spr-5(by134) compared with controls. While SOD1G85R protein levels are unchanged in the S fraction, those in the P fraction are decreased in the double suppressor mutant. The P fraction represents only about 1.7% of total SOD1G85R in the WT sample; therefore, a higher ratio of the P fraction relative to the S fraction (approximately 2:1) is used for the western analysis."
NCBI gene IDs:
"SOD1: 6647
spr-5: 173214
ufd-2: 174295"
Figure legend: "(H) The overexpression (OE) of WT UFD-2 or SPR-5 in the C. elegans nervous system blocks the protection conferred by the double mutation ufd-2(tm1380);spr-5(by134) (n = 16). Data represent means ± SEM. The numerical data used to make this figure can be found in S1 Data."
NCBI gene IDs:
"spr-5: 173214
SPR-5: 173214
ufd-2: 174295
UFD-2: 174295"
Figure legend: "(A) Top: schematic drawing depicts pan-neuronal expression of YFP in head and ventral neurons in the context of the C. elegans body plan. Left: micrographs show the SOD1G85R-YFP proteins expressed in WT or the spr-5(by134);ufd-2(tm1380) mutant background. The double-mutant worms show a marked decrease in protein aggregation in neurons. Enlarged sections of headneurons (red framed) and ventral cord neurons (white framed) are shown. Middle: quantification of locomotion in the spr-5(by134);ufd-2(tm1380) and the WT C. elegans with neuronal expression of SOD1G85R-YFP (n = 30). Right: a decrease in the protein levels of SOD1G85R-YFP in the presence of spr-5(by134);ufd-2(tm1380) is shown by western blots of the supernatant (S) and the pellet (P) fractions. (B and C) Analyses of the spr-5(by134);ufd-2(tm1380) and the WT C. elegans with neuronal expression of TDP-43-c25-YFP (n = 30) or PolyQ-YFP (n = 12) as in (A)."
NCBI gene IDs:
"SOD1: 6647
spr-5: 173214
ufd-2: 174295"
Figure legend: "(D) Neurodegenerative rough-eye phenotype in adults is alleviated by the knockdown of the Drosophila homologs of ufd-2/UBE4B and spr-5/LSD1-CG9934 and Su(Var)3-3, respectively, compared to the control (CTRL). Eye-specific expression of TDP-43M337V, FUSR521C, and RNA interference (RNAi) was driven by GMR-Gal4."
NCBI gene IDs:
"GMR-Gal4: 
CG9934: 34699
FUS: 2521
LSD1: 23028
spr-5: 173214
Su(Var)3-3: 40217
TDP-43: 23435
UBE4B: 10277
ufd-2: 174295"
Figure legend: "(A) Western blots of cell lysates derived from mock (CTRL), single UBE4B or LSD1, or double UBE4B and LSD1 knockdowns. Supernatant (S) and pellet (P) fractions were probed with indicated antibodies. While the LSD1 or UBE4B single-knockdown reduces the SOD1G85R aggregates in both supernatant and pellet fractions, the combined knockdown produces the strongest reduction in the aggregates. (B) Quantification of SOD1G85R protein levels by western blotting (A). n = 3 (supernatant); n = 8 (pellet)."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(C) Western blots of a representative cycloheximide chase experiment to determine SOD1 protein half-lives in the double UBE4B and LSD1 knockdown cells versus controls. (D) Quantification of SOD1G85R clearance, as analyzed by western blotting in (C). The graph indicates the relative band intensity of SOD1G85R at each chase time point. n = 5; Overall p = 0.02 (paired t test, CTRL versus UBE4B and LSD1 double knockdown). Individual p = 0.03 (3 h), p = 0.003 (6 h), p = 0.06 (9 h), and p = 0.004 (12-21 h)."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(E) The half-life of SOD1G85R is reduced from 8.5 h to 5 h upon knockdown of UBE4B and LSD1. Data represent means ± SEM. The numerical data used to make this figure can be found in S1 Data."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(B) The volcano scatter plot indicates fold changes in the levels of gene transcripts affected differentially by the UBE4B and LSD1 double knockdown versus control shRNA. Gray spots represent 22,148 annotated transcripts. Red spots are predicted p53-activated targets, and blue spots are predicted p53-inhibited targets. The enrichment of red spots in the up-regulated genes (right upper quadrant, &gt;1.2-fold change, p ≤ 0.04) and blue spots in the down-regulated genes (left upper quadrant) indicates that the p53-mediated transcription is activated by the UBE4B and LSD1 double knockdown."
NCBI gene IDs:
"LSD1: 23028
p53: 7157
UBE4B: 10277"
Figure legend: "C) RT-qPCR validation of the expression levels of representative p53 target genes in the UBE4B and LSD1 double-knockdown samples. n = (2 to 6), p ≤ 0.04."
NCBI gene IDs:
"LSD1: 23028
p53: 7157
UBE4B: 10277"
Figure legend: "(D) The p53 protein level is significantly increased by UBE4B and LSD1 double knockdown. Left: representative western blots showing p53 levels, the knockdown of UBE4B and LSD1, and the actin control in HEK293T cells. Right: quantification of the p53 protein levels normalized against actin (n = 3)."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "E) The p53-mediated transcriptional activity is measured by a luciferase reporter under the control of a p53-response element promoter, which was transfected into HEK293T cells 72-96 h after the initiation of the UBE4B and LSD1 single or double knockdowns (n = 6)."
NCBI gene IDs:
"LSD1: 23028
p53: 7157
UBE4B: 10277"
Figure legend: "(F) Increased interaction of p53 with its activating partner 53BP1 in response to UBE4B and LSD1 knockdown. Left: representative western blots of 53BP1 co-immunoprecipitation. Right: quantification of the 53BP1 co-immunoprecipitation (n = 2). Data represent means ± SEM."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(G) RT-qPCR validation of the expression levels of FOXO3a, FOXO4, and PSMD11 (n = 6)."
NCBI gene IDs:
"FOXO3a: 2309
FOXO4: 4303
PSMD11: 5717"
Figure legend: "(H) The FOXO3a-mediated transcriptional activity was measured with a luciferase reporter under the control of a FOXO-response element promoter. HEK293T cells were transfected with shRNAs for control, LSD1, UBE4B, or both LSD1 and UBE4B, followed by transfection of the luciferase reporter together with a constitutively active form of FOXO3a (TM) 72-96 h later (n = 6). Data represent means ± SEM. The numerical data used to make this figure can be found in S1 Data."
NCBI gene IDs:
"FOXO3a: 2309
LSD1: 23028
UBE4B: 10277"
Figure legend: "(A) Increased protein levels of proteasome subunits upon the UBE4B and LSD1 double knockdown in HEK293T cells"
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: ". (B) The UBE4B and LSD1 single or double knockdowns increase the proteasomal degradation of a reporter substrate, Suc-LLVY-Luciferin, whose degradation is measured in a luciferase release assay. The double knockdown shows synergistic proteasomal activation when compared with the individual knockdowns (n = 6)."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(C) The autophagy activity is significantly increased in cells with the UBE4B and LSD1 double knockdown. The in vivo activity of the ATG4B protease, which cleaves LC3 precursors, was quantified via a Gaussia luciferase release assay (see S5D Fig., S5E Fig., and Materials and Methods for details). The HCT116 cells were analyzed 48 h after the initiation of the knockdown."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(D) Quantification of LC3-II levels in HEK293T cells with the UBE4B and LSD1 double knockdown or the mock control. The cells were treated with or without 10 mM 3-methyladenine (3-MA) for 48h before lysis. Western blots indicate an increase in LC3 levels (top panels) in the double UBE4B and LSD1 knockdown cells (middle panels), while the actin control is unchanged (bottom panels). The graph shows the quantification of LC3-II levels as normalized to actin levels (n = 3). A one-way ANOVA test with matched multiple comparisons and Tukey correction was used for statistics. Data represent means ± SEM. The numerical data used to make this figure can be found in S1 Data."
NCBI gene IDs:
"LSD1: 23028
UBE4B: 10277"
Figure legend: "(A) p53 small molecule activators Tenovin-1 and CP-31398 reduce the levels of misfolded SOD1 proteins, as determined by the SOD1G85R solubility assay in HEK293 cells. Increasing concentrations of the p53 activators significantly decrease the levels of SOD1G85R but not the endogenous WT SOD1 proteins in western blots of both supernatant and pellet fractions."
NCBI gene IDs:
"SOD1: 6647
p53: 7157"
Figure legend: "(B) A decrease in p53 as the result of shRNA knockdown increases the levels of SOD1G85R but not WT SOD1 proteins in the SOD1G85R aggregation assay, as shown by western blots of both supernatant (S) (n = 2) and pellet (P) (n = 3) fractions."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(C) A complete absence of p53 increases the accumulation of SOD1G85R mutant proteins in p53-/- HCT116 cells when compared with controls. Representative western blots (left panels) and quantification of SOD1G85R levels in the supernatant lysates are shown. The middle graph indicates the ratio of G85R to WT SOD1 proteins in the presence or absence of p53 with varying amounts of transfected mutant SOD1. The right graph panel shows the same data as shown in the middle panel, but normalized to the average SOD1G85R level for each amount of the transfected plasmid. Data represent means ± SEM. The numerical data used to make this figure can be found in S1 Data."
NCBI gene IDs:
"SOD1: 6647
p53: 7157"
Figure legend: "(A) Left: p53 knockdown reverses the enhanced clearance of SOD1G85R proteins conferred by the UBE4B and LSD1 double knockdown. Total amounts of shRNAs were adjusted to be equal with nontargeting CTRL shRNAs. Right: quantification of insoluble aggregated SOD1G85R in pellet fractions from HEK293T cells transfected with control, double (UBE4B/LSD1), or triple (UBE4B/LSD1/p53) shRNAs (n = 2)."
NCBI gene IDs:
"LSD1: 23028
SOD1: 6647
p53: 7157
UBE4B: 10277"
Figure legend: "(B) The degenerative TDP-43M337V eye phenotype is exacerbated by the knockdown of the Drosophila homolog of p53 (p53 RNAi), or by the overexpression of a dominant-negative p53 mutant (p53.R155H). Expression of p53 RNAi, p53.R155H, and TDP-43M337V are driven by GMR-Gal4."
NCBI gene IDs:
"GMR-Gal4: 
p53: 2768677
TDP-43: 23435"
Figure legend: "(C) The p53-activating drug Tenovin-1 (TEN1) protects spinal cord motor neurons from SOD1G85R-induced proteotoxicity. Rat spinal cord cultures were grown as described in Materials and Methods and treated with 0.8 μM TEN1 or vehicle (VEH) DMSO, followed by infection of HSV-SOD1G85R or the HSV-LacZ control after 24 h. At day 5 post-infection, cells were fixed and stained with a motor neuron-specific anti-neurofilament H (NF-H) antibody. Left: representative images of motor neurons in each condition. Scale bar = 50 μm. Right: quantification of motor neuron survival (one-way ANOVA with multiple comparisons and Tukey correction). Data represent means ± SEM."
NCBI gene IDs:
"LacZ: 
SOD1: 6647
p53: 24842"
Figure legend: "Survival curves for control (n=23 biological replicates), Rorc(t)CreTg Tet1/3fl/fl(n=20 biological replicates), Rorc(t)CreTg Tet1/2/3fl/fl mice (n=10 biological replicates). Paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463"
Figure legend: "Representative H&amp;E staining of the lungs (top panel) and small intestine (bottom panel) and histopathological scoring of 3 months old control (n=3 biological replicates) and Rorc(t)CreTg Tet1/3fl/fl mice (n=3 biological replicates). Data are means +/-SEM, One-Way ANOVA with uncorrected Fisher's LSD test. Scale bar = 263 and 53 µm, top and bottom panels, respectively. , Paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463"
Figure legend: "Absolute number of FoxP3+ Tregs from the thymus (n=5 or 7 biological replicates) and spleen (n=7 or 8 biological replicates) of control Tet1/3fl/fl and Rorc(t)CreTg Tet1/3fl/fl mice. Data is means +/- SEM, unpaired Mann-Whitney t test. Paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463"
Figure legend: "Quantification of CD25 MFI expression on FoxP3+ T cells in the spleen of control Tet1/3fl/fl (n=4 biological replicates) and Rorc(t)CreTg Tet1/3fl/fl mice (n=4 biological replicates). Data is means +/- SEM, unpaired Mann-Whitney t test. Paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463"
Figure legend: "Quantification of FoxP3 MFI expression on FoxP3+ T cells in the spleen of control Tet1/3fl/fl (n=6 biological replicates) and Rorc(t)CreTg Tet1/3fl/fl mice (n=6 biological replicates). Data is means +/- SEM, unpaired Mann-Whitney t test.   Quantification of ICOS MFI expression on FoxP3+ T cells from the mesenteric lymph nodes of control Tet1/3fl/fl (n=5 biological replicates) and Rorc(t)CreTg Tet1/3fl/fl (n=7 biological replicates) mice. Data is means +/- SEM, unpaired Mann-Whitney t test.   , Paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463"
Figure legend: "Weight curve of female Rag-/- mice after T-cell transfer colitis induced via transfer of naïve WT CD45.1 conventional CD4+ T cells alone (n=5 recipients) or in combination with Tregs isolated from Tet1/3fl/fl and Rorc(t)CreTg Tet1/3fl/fl littermates (n=5 recipients/group). Data is means +/- SEM, Two-Way ANOVA with Geisser-Greenhouse correction. Experiment was replicated 2 times."
NCBI gene IDs:
"Cre: 2777477
CD45.1: 19264
Rag: 19373
Rorc: 19885
Tet1: 52463"
Figure legend: "Representative flow plots and quantification of percent Tbet+, Rorγt+ and FoxP3+ of CD45.1 CD4+ effector cells isolated from the MLN of female Rag-/- mice 7 weeks post T cell transfer induced colitis. Respective cohorts were Naïve CD45.1 T cells only (n=5 mice), WT CD45.1+ Tet1/3fl/fl Tregs (n=5 mice) and WT CD45.1+Rorc(t)CreTg Tet1/3fl/fl Tregs (n=4 mice). Cells were pre-gated on Live CD45.1+TCRβ+ cells. Data is means+/- SEM, Two-Way ANOVA with Tukey's Multiple comparison test. Experiment was replicated 2 times."
NCBI gene IDs:
"Cre: 2777477
CD45.1: 19264
Rag: 19373
Rorc: 19885
Tet1: 52463"
Figure legend: "Quantification of Live CD4+ FoxP3+ T cells absolute cell numbers in different organs of FoxP3Cre Tet1/3+/+ (n=3 biological replicates) and FoxP3Cre Tet1/3 fl/fl (n=3 biological replicates) male littermate mice. Data is shown as means+/- SEM, One-Way ANOVA with uncorrected Fisher's LSD test."
NCBI gene IDs:
"Cre: 2777477
FoxP3: 20371
Tet1: 52463"
Figure legend: "Quantification of CD25-FoxP3lo, CD25+FoxP3- and CD25+FoxP3+ cells among Live CD73-CD4+ T cells thymocytes of control Tet3fl/fl (n=5 biological replicates) and Rorc(t)CreTg Tet3fl/fl mice (n=3 biological replicates) mice. Data is shown as means+/- SEM, Two-Way ANOVA with Sidak's multiple comparison test. Quantification of CD25-FoxP3lo, CD25+FoxP3- and CD25+FoxP3+ cells among Live CD73-CD4+ T cells thymocytes of control Tet1fl/fl (n=6 biological replicates) and Rorc(t)CreTg Tet1fl/fl mice (n=6 biological replicates). Data is shown as means+/- SEM, Two-Way ANOVA with Sidak's multiple comparison test. paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Tet1: 52463
Tet3: 194388"
Figure legend: "Quantification of Nur77 staining among TCRβhi CD69+ CD4 SP thymocytes from control Tet3fl/fl (n=5 biological replicates) and Rorc(t)CreTg Tet3fl/fl mice (n=3 biological replicates). Data is as means+/- SEM, unpaired two-tailed Mann-Whitney t-test Quantification of Nur77 staining among TCRβhi CD69+ CD4 SP thymocytes from control Tet1fl/fl (n=4 biological replicates) and Rorc(t)CreTg Te1fl/fl mice (n=3 biological replicates). Data is shown as means+/- SEM, unpaired two-tailed Mann-Whitney t-test paired male or female mice were used and no gender biases associated with genotypes were observed."
NCBI gene IDs:
"Cre: 2777477
Rorc: 19885
Te1: 52463
Tet1: 52463
Tet3: 194388"
Figure legend: "IL-2 mRNA quantification by qPCR in CD4+ T cells post-activation at indicated time-points (n=2 biological replicates/genotype). Cells were stimulated with anti-CD3/CD28 without exogenous IL-2 or TGF-β. Data is shown as means+/-SEM, Two-Way ANOVA with Sidak's multiple comparison test"
NCBI gene IDs:
"IL-2: 16183"
Figure legend: "Integrated genome viewer (IGV) browser tracks of the Lck, Fyn gene loci highlighting differential accessible regions (in red boxes) in biological replicates of CD4+ T cells activated with anti-CD3/CD28 and 20 ng/mL TGF-β +/- 100U IL-2."
NCBI gene IDs:
"Fyn: 14360
Lck: 16818"
Figure legend: "Integrated genome viewer (IGV) browser tracks of the Itk gene loci highlighting differential accessible regions (in red boxes) in biological replicates of CD4+ T cells activated with anti-CD3/CD28 and 20 ng/mL TGF-β +/- 100U IL-2."
NCBI gene IDs:
"Itk: 16428"
Figure legend: "(G) IGV browser track of the IL-2 locus showing accessibility peaks at the IL-2 promoter region and upstream of the promoter (red arrow) in biological replicates of CD4+ T cells activated as in (B)."
NCBI gene IDs:
"IL-2: 16183"
Figure legend: "(L) Larval NSCs of control (ctrl; yw), pr-set7716/Df, and UAS-pr-set7 induced with insc­-Gal4 at 72h ALH were stained for Pr-set7, Mira and DNA. (M, N) Quantifications of NSC diameters (M) at 24h ALH and EdU incorporation (N) for genotypes in (O). In (M), n=152 for control; n=129 for pr-set7716/Df. In (N), for control, n=601, 13 BL at 24h ALH; n=988, 14 BL at 48h ALH; n=813, 9 BL at 72h ALH and n=829, 10 BL at 96h ALH. For pr-set7716/Df, n=566, 12 BL at 24h ALH; n=808, 13 BL at 48h ALH; n=1032, 10 BL at 72h ALH and n=825, 9 BL at 96h ALH. Data information: Quantification data are presented in as mean ± SD M, N). n numbers in N) were total NSCs counted with total brain lobes measured, while each brain lobe considered as one biological replicate. White arrows point to NSCs Statistical analyses were done comparing two different genotypes using Mann-Whitney test M, N). ***P &lt; 0.001. Scale bars, 5 μm L),"
NCBI gene IDs:
"Gal4: 855828
pr-set7: 41743"
Figure legend: "(C) Larval NSCs of control (ctrl; yw), pr-set7716/Df, pr-set7716/Df with UAS-pr-set7 induced with insc-Gal4, and pr-set7716/Df with pr-set7-BAC at 24h ALH were stained for H4K20me1, Dpn, Mira and DAPI. Control: n=108; pr-set7716/Df: n=87; pr-set7716/Df with UAS-pr-set7 induced with insc-Gal4: n=167 and pr-set7716/Df with pr-set7-BAC: n=104. Data information: White arrows point to NSCs. Scale bars, 5 μm"
NCBI gene IDs:
"Gal4: 855828
pr-set7: 41743"
Figure legend: "(E) Larval NSCs with FUCCI constructs induced with insc-Gal4 at 24h were stained for GFP, RFP, Dpn, and Prospero (Pros). (F) Quantifications of quiescent NSCs in different cell-cycle stages from FUCCI flies (induced with insc-Gal4) in control (and insc-Gal4&gt;FUCCI) and pr-set7716/Df. Quiescent NSCs of insc-Gal4&gt;FUCCI: 76.1%±1.5% in G2, 1.7%±0.2% in S and 22.2%±1.3% in G0 (n=343). Quiescent NSCs of insc-Gal4&gt;FUCCI; pr-set7716/Df: 74.4%±1.0% in G2, 0.9%±0.2% in S and 24.7%±0.8% in G0 (n=344). Data information: Quantification data are presented in as mean ± SD Statistical analyses were done comparing two different genotypes using Mann-Whitney test ns: non-significant. White arrows point to NSCs. Scale bars, 5 μm"
NCBI gene IDs:
"Gal4: 855828
pr-set7: 41743"
Figure legend: "(D) qPCR analysis of pr-set7, cdk1, ebd1 and arm in larval brains of control (ctrl; yw) and pr-set7716/Df(3R)ED5644 (716/Df) at 24h ALH. n=7 as biological replicates. Statistical analyses were done comparing between control (GAPDH1, GAPDH2, Tbp1 and Rpl32) and pr-set7716/Df, using Mann-Whitney test. The mRNA levels of control were normalized to 1. Data are presented as mean ± SD calculated from all replicates. Data information: Data are presented in (D, as mean ± SD. Statistical analyses were done comparing two different genotypes using Mann-Whitney test (D *P &lt; 0.05"
NCBI gene IDs:
"GAPDH1: 
GAPDH2: 
Rpl32: 
Tbp1: 
arm: 31151
cdk1: 34411
ebd1: 38082
pr-set7: 41743"
Figure legend: "G) Larval NSCs of control (ctrl; yw) and pr-set7716/Df at 24h ALH were stained for Ebd1, Dpn and Mira. Yellow dotted boxes, zoomed-in area. White arrows point to NSCs. Data information: Scale bars, 15 μm (G)."
NCBI gene IDs:
"pr-set7: 41743"
Figure legend: "(H, I) Quantifications of nuclear Ebd1 (H) and the corrected total cell fluorescence (CCF) (I) In (H), n=756, 9 BL for control; n=765, 9 BL for pr-set7716/Df. In (I), CCF = Integrated Density - (Area of selected cell × Mean fluorescence of background readings). CCFs were 1102.2±493.2 (n=99) for control (ctrl; yw) and 144.0±88.0 (n=98) for 716/Df. Data information: Data are presented in , I, s mean ± SD. n numbers in (H) were total NSCs counted with total brain lobes measured, while each brain lobe was considered as one biological replicate. Statistical analyses were done comparing two different genotypes using Mann-Whitney test , H, I, ***P &lt; 0.001."
NCBI gene IDs:
"pr-set7: 41743"
Figure legend: "(H) Larval brains of control (ctrl; yw), cdk1-Myc overexpression under insc­-Gal4, pr-set7716/Df(3R)ED5644 (716/Df), and pr-set7716/Df with cdk1-Myc induced with insc-Gal4 at 24h ALH were labelled with Dpn and EdU. (I, J) Quantifications of EdU incorporation (I) and NSC diameters (J) for genotypes in (H). In (I), n=1334, 16 BL for control; n=1299, 15 BL for insc&gt;cdk1; n=1221, 18 BL for pr-set7716/Df and n=1372, 16 BL for pr-set7716/Df with cdk1-Mycinduced with insc-Gal4. In (J), n=153 for control; n=152 for insc&gt;cdk1; n=156 for pr-set7716/Df and n=159 for pr-set7716/Df with cdk1-Myc induced with insc-Gal4.Data information: Data were presented in quantification graphs as mean ± SD I, J). n numbers in I) were total NSCs counted with total brain lobes measured, while each brain lobe was considered as one biological replicate. Statistical analyses were done comparing among different genotypes using ordinary one-way ANOVA ***P &lt; 0.001. Scale bars, 30 μm H). Control: insc­-Gal4/+ UAS-dicer2"
NCBI gene IDs:
"cdk1: 34411
Gal4: 855828
pr-set7: 41743"
Figure legend: "(A) Larval NSCs of control (ctrl; yw) and ebd1240 at 24h ALH were stained for Deadpan (Dpn) and Miranda (Mira). (B) Quantifications of cellular extensions for genotypes in (A). Control: n=886, 11 BL; ebd1240: n=864, 12 BL. (C) Larval brains of control (ctrl; yw) and ebd1240 at 24h ALH were labelled with Dpn and EdU. (D) Quantifications of NSC without EdU incorporation for genotypes in (C). Control: n=1034, 12 BL; ebd1240: n=1139, 16 BL. Data information: Data are presented in (B, D as mean ± SD. n numbers in (B, D, were total NSCs counted with total brain lobes measured, while each brain lobe was considered as one biological replicate. Statistical analyses were done comparing two different genotypes using Mann-Whitney test (B, D ns: non-significant; *P &lt; 0.05; ***P &lt; 0.001."
NCBI gene IDs:
"ebd1: 38082"