EMBO/SourceData huggingface dataset:
Figure legend: "Quantification of microneme secretion in control and Tg277910-depleted tachyzoites was measured as in Fig 2H. Blots were probed with anti-MIC2 (secretion of micronemes) and anti-GRA3 (constitutive secretion of dense granules). P: Parasites pellet. Sup: Supernatant from untreated parasites. Sup+Prop: Supernatant from parasites treated with propranolol. The results are representative of two independent experiments."
NCBI gene IDs:
"277910: 7900354"
Figure legend: "Immunofluorescence images of extracellular tachyzoites of untagged, TgCRMPa-HA3 and TgCRMPb-HA3 parasites, incubated either with host cell monolayers for 2min, or with ionophore A23187, to induce natural or artificial conoid extrusion, respectively. Parasites were immunostained with anti-HA Abs; DNA was labeled by Hoechst. CRMPa and CRMPb consistently accumulate at the tip of extruded conoids (arrows). The apexes of A23187-treated parasites were magnified on the right, and increased in brightness and contrast to highlight the apical dots. DIC: differential interference contrast. Quantification of the dot pattern upon A23187 treatment shown in (A). Values are expressed as percentage of parasites showing (dot) or lacking (no dot) the apical accumulation of TgCRMPa and TgCRMPb; n= number of parasites analyzed per line."
NCBI gene IDs:
"HA3: 
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "Immunofluorescence images of extracellular TgCRMPb-HA3 tachyzoites in presence (-ATc) or absence (+48h ATc) of TgCRMPa-FLAG3 (iKD line). Parasites were stained with anti-HA Abs to visualize CRMPb. TgCRMPb localization at the tip of the extruded conoid (arrow) disappears upon TgCRMPa depletion, but it is still detected in the cytoplasm (lower panel). DNA is labeled by Hoechst. Single focal planes are shown. DIC: differential interference contrast.   Quantification of the dot pattern shown in (C). The values are reported as in (B).  "
NCBI gene IDs:
"FLAG3: 
HA3: 
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "Whole cell lysates from TIR1-expressing parental line (Ctrl), HA3-miniAID-TgCRMPa_iKD (N-term) and TgCRMPa-miniAID-HA3_iKD (C-term) lines were immunoblotted with anti-HA Abs to visualize tagged CRMPa in IAA-treated and untreated samples. CRMPa was undetectable upon 24h incubation with IAA when C-terminally, but not N-terminally, tagged with the miniAID-HA3, suggesting that the C-terminus is the one exposed towards the cytosol. TgMIC2 was used as loading control and detected with anti-MIC2 Abs. MW: molecular weight standards."
NCBI gene IDs:
"HA3: 
TIR1: 
CRMPa: 7894778"
Figure legend: "Immunofluorescence images of extracellular A23187-treated parasites expressing either N-or C-terminally HA3-tagged TgCRMPa, and immunostained as in (A). TgCRMPa accumulates at the tip of extruded conoids (arrows) in triton-permeabilized (+) or non-permeabilized (-) parasites. DIC: differential interference contrast. Single focal planes are shown.   Quantification of the dot pattern upon natural (+H F Fibroblasts) or artificial (+A23187) conoid extrusion in parasites expressing either N- or C-terminally HA3-tagged TgCRMPa. Values are reported as in (B).  "
NCBI gene IDs:
"HA3: "
Figure legend: "Ultrastructure Expansion Microscopy of extracellular tachyzoites, either untagged or co-expressing TgCRMPa-HA3/TgCRMPa-TY2 and TgCRMPb-HA3 together or in pairwise combination with TgNd6-TY2. Parasites were treated with A23187 prior to fixation and preparation for U-ExM, and stained with anti-HA, anti-TY and anti-α/β tubulin Abs to label CRMPs, Nd6/CRMPa and microtubules, respectively. Shown are maximum intensity projections of z-stack confocal images. CRMPs overlap with Nd6 at the tip of the extruded conoid (yellow selection). A magnified image of the apical tip of each parasite is shown on the right."
NCBI gene IDs:
"HA3: 
TY2: 
Nd6: 7897938
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "Extent of colocalization between TgCRMPa-TY2 and TgCRMPb-HA3, and TgCRMPs-HA3 with TgNd6-TY2 in the apical dot shown in B. Pearson's correlation coefficient was measured using the Fiji-JACoP plugin. Values are expressed as mean ± SD. Three-five parasites per line were analyzed. Quantification of the dot pattern for TgCRMPa-HA3 and TgCRMPb-HA3 in TgNd9_iKD lines. CRMPs accumulation at the apical tip of extracellular parasites was measured as in Fig EV4A. TgCRMPs were still found in the apical dot in absence of TgNd9 (+ATc). Numbers are expressed as percentage of parasites showing (dot) or lacking (no dot) the tip accumulation of TgCRMPa and TgCRMPb. The number of parasites (n) analyzed for each line is reported at the top of the column."
NCBI gene IDs:
"Nd6: 7897938
Nd9: 7899494
CRMPa: 7894778
CRMPb: 7897818"
Figure legend: "(A-D) Relative growth  of (top) or western blot  analysis of YAP  in (bottom) the indicated prostate cancer  cell lines expressing control, YAP-WT, or YAP-5SA constructs through lentiviral  infection. Of note, LNCaP and C4-2 express full-length AR  while 22RV1 and R1-D567  express AR  splicing variants V7 and V567ES, respectively. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"AR: 367
YAP: 10413"
Figure legend: "(M) Western blot  analysis of LATS1, LATS2, YAP, phosphorylated YAP  at S109 (pS109) and S127 (pS127) in C4-2 cells treated with two independent LATS1/2 siRNAs."
NCBI gene IDs:
"LATS1: 9113"
Figure legend: "(E) Relative mRNA levels of AR  and the indicated AR  target genes (left) and western blot analysis of AR  (right) in  C4-2 cells treated with increasing concentrations of XMU-MP-1. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(G) Relative mRNA levels of the indicated AR  or YAP  target genes in  C4-2  cells treated with control (siNC) or YAP  siRNA (siYAP_1 or siYAP_2) for 24 hours and with or without 2 μM XMU-MP-1  for 12 hours. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "Relative mRNA levels of the indicated AR  or YAP  target genes (J, in C4-2 (J, cells expressing control (NC), YAP-WT or YAP-5SA constructs through lentiviral  infection. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "Western blot analysis of AR  and YAP  (I, in C4-2  cells stably expressing Tet-O-YAP-5SA and treated with 0.2 μg/ml doxycycline  (Dox) for the indicated periods of time. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "Western blot analysis of AR  and YAP  L) in C4-2  cells stably expressing Tet-O-YAP-5SAS94A and treated with 0.2 μg/ml doxycycline  (Dox) for the indicated periods of time."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "relative mRNA levels of TEAD1  and TEAD4  and the indicated AR  target genes (B) C4-2 cells treated with two independent TEAD1/3/4 siRNA or control siRNA. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"TEAD1: 7003
TEAD4: 7004"
Figure legend: "(D) Relative mRNA levels of the indicated AR  target genes (left) and western blot  analysis of TEAD  (right) in C4-2  cells expression the control or TEAD4  constructs and treated with vehicle or XMU-MP-1. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"TEAD4: 7004"
Figure legend: "HEK-293T  cells transfected with the indicated AR, TEAD constructs. Cell lysates were immunoprecipitated  with the indicate antibodies. Immunoprecipitates  and cell lysates (input) were analyzed by  western blot  with the indicated antibodies."
NCBI gene IDs:
"AR: 367"
Figure legend: "(O-P) ChIP-qPCR  (O) and  RT-PCR analyses show that exogenous expression of wild type (TEAD4-WT) but not the DNA binding  deficient form (TEAD4m) of TEAD4  rescued AR binding  to the promoter/enhancer regions of KLK2  and KLK3  (O) as well as the expression of these genes (P) in  C4-2  cells treated with TEAD1/3/4 siRNA. Data information: Results are representatives of three independent experiments. Data are ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"KLK2: 3817
KLK3: 354
TEAD1: 7003
TEAD4: 7004"
Figure legend: "(D) YAP-5SA but not YAP-5SAS94A inhibited AR/TEAD interaction. C4-2  cells stably expressing Tet-O-GFP, Tet-O-YAP-5SA or Tet-O-YAP-5SAS94A were treated with 0.2 μg/ml doxycycline  (Dox) for 10 hours, followed by CoIP  and western blot  analyses with the indicated antibodies."
NCBI gene IDs:
"GFP: 
YAP: 10413"
Figure legend: "(G-H) ChIP-qPCR analysis of AR  (G) and TEAD  (H) binding  to the promoter/enhancer regions of KLK2, KLK3  and CTGF  in Tet-O-YAP-5SA expressing  C4-2 cells treated with 0.2 μg/ml doxycycline  (Dox) for the indicated time. Data information: Results are representatives of three independent experiments. Data ± SD. ns: not significant, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"CTGF: 1490
KLK2: 3817
KLK3: 354
YAP: 10413"
Figure legend: "Western blot  analysis of YAP  and TEAD  (left) and mRNA levels of the indicated genes (right) in C4-2  cells treated with siYAP/TAZ  for 18 (A), 36 (B), hours. Data information: Results are representatives of three independent experiments. Data ± SD. ns: not significant, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"TAZ: 6901
YAP: 10413"
Figure legend: "(A-F) YAP  activation inhibited AR  positive  prostate cancer  growth in xenografts  bearing C4-2  or 22RV1 tumors  that stably express Tet-O-YAP-5SA. Male NOD scid gamma (NSG) mice  bearing C4-2 tumor  cells stably expressing a Tet-O-EGFP  construct were used as control. Male NOD scid gamma (NSG) mice  bearing the indicated tumors  were injected i.p. with PBS  containing Dox  (20mg/kg) or PBS  daily for the indicated time. Tumor growth  curve (A, D), photograph of tumor  samples (B, E), and quantification of  tumor weight  (C, F) at the end of treatment were shown. XMU-MP-1 inhibited AR  positive  prostate cancer  growth in xenografts  bearing C4-2  (G-I), tumors. Male NOD scid gamma (NSG) mice  bearing the indicated tumors  were treated daily with XUM-MP-1 at the indicated concentrations or with vehicle for the indicated periods of time. Tumor growth  curve (G, J, images of tumor samples (H, K, and quantification of tumor weight (I at the end of treatment were shown. Data information: Data are ± SD. **P&lt;0.01, ***P&lt;0.001 (Student's t-test). n=7 mice  for each group."
NCBI gene IDs:
"EGFP: 
YAP: 10413"
Figure legend: "(C) Long-range PCR assay to detect mtDNA deletions in TOP3A patient muscle (lanes 2-5). Samples from unaffected individuals (lanes 6 and 7) and a single-deletion mitochondrial disease patient (lane 8) are used as negative and positive controls, respectively. 'M' indicates marker."
NCBI gene IDs:
"TOP3A: 7156"
Figure legend: "(A-B) Analysis of mtDNA structure in muscle from individuals with TOP3A-related mitochondrial disease using Southern blotting following restriction with BamHI (A) or PvuII (B). The black bar indicates the probe (nt. 16,262 - 128)."
NCBI gene IDs:
"TOP3A: 7156"
Figure legend: "mtDNA phenotypes in U2OS Flp-In cells treated with TOP3A siRNA, rescued using variant-containing siRNA-resistant forms of TOP3A. (C) Western blot of TOP3A protein expression. Cells were untransfected (lane 1) or transfected with TOP3A siRNA (lanes 2-11), then induced to express WT TOP3A (lane 3) or pathological variants of TOP3A (lanes 4-11). Note that TOP3A Ser810* does not carry a C-terminal HA tag. β-actin is used as a loading control."
NCBI gene IDs:
"TOP3A: 7156"
Figure legend: "mtDNA phenotypes in U2OS Flp-In cells treated with TOP3A siRNA, rescued using variant-containing siRNA-resistant forms of TOP3A. (D) mtDNA copy number for TOP3A rescue cells as in (C) determined using qPCR. Error bars represent SEM, n = 3 (Ala95Val) or n = 4 (all other samples), where each data point represents the mean of 3 technical triplicates. Significance values are shown for one-way ANOVA compared to untreated wild-type cells, *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, ****p&lt;0.0001."
NCBI gene IDs:
"TOP3A: 7156"
Figure legend: "mtDNA phenotypes in U2OS Flp-In cells treated with TOP3A siRNA, rescued using variant-containing siRNA-resistant forms of TOP3A. (E) Southern blot of mtDNA structure from cells as in (C). The probe (black bar, nt. 16,262 - 128) detects both full-length (FL) mtDNA and 7S DNA. The presence of hemicatenated mtDNAs is indicated. (F) Southern blot of uncut mtDNA to visualise different topological forms, from cells as in (C). Control DNA treated with BamHI (lane 1) or E. coli topoisomerase I (lane 2) act as markers for linear and relaxed monomeric mtDNA, respectively. Diagrams indicate the migration of different mtDNA forms."
NCBI gene IDs:
"TOP3A: 7156"
Figure legend: "The rate of glucose uptake (a,e), lactate released after 4 h of incubation (b,f), glycolytic flux (c,g) and pentose-phosphate flux (d,h) were assessed in MEF and mouse cortical neurons in primary culture, as indicated, obtained from either WT or Pink1 KO (Pink1−/−) mice."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "Blood concentrations of glucose (i) and lactate (j), and the rate of 14C-lactate appearance in the blood from acutely injected [U-14C]glucose in the tail vein (k) of WT and Pink1 KO mice were analysed. Data are expressed as mean±s.e.m. *P0.05 (Student's t-test; n=3-4 independent experiments)."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "Quantitative real-time PCR (RT-qPCR analysis of the relative mRNA abundances of Glut1, Glut3, Hk2 and Gapdh in WT and Pink1 KO miceMEF (a) ß-Actin mRNA was used for normalization, and the Pink1 KO data are expressed as the change relative to WT."
NCBI gene IDs:
"ß-Actin: 
Gapdh: 407972
Hk2: 15277
Pink1: 68943
Glut1: 20525
Glut3: 20527"
Figure legend: "Western blot analysis of the protein abundances of PINK1, GLUT1, GLUT3 (not detectable in MEF), HK2, GAPDH and HIF1α in WT and Pink1-null mice MEF (b) ß-Actin was used as loading control."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "Quantitative real-time PCR (RT-qPCR analysis of the relative mRNA abundances of Glut1, Glut3, Hk2 and Gapdh in WT and Pink1 KO neurons (c). ß-Actin mRNA was used for normalization, and the Pink1 KO data are expressed as the change relative to WT."
NCBI gene IDs:
"ß-Actin: 
Gapdh: 407972
Hk2: 15277
Pink1: 68943
Glut1: 20525
Glut3: 20527"
Figure legend: "Western blot analysis of the protein abundances of PINK1, GLUT1, GLUT3 (not detectable in MEF), HK2, GAPDH and HIF1α in WT and Pink1-null neurons (d); ß-Actin was used as loading control."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(e) Western blot against PINK1, GLUT1, GLUT3 and HIF1α in WT and Pink1 KO mice skeletal muscle samples; α-Tubulin was used as loading control. Only representative western blots are shown per condition; the replicates and the semi-quantitative estimation of the band intensities are shown in the Supplementary Information. The mean±s.e.m. values of the mRNA data were calculated from the fold change of each ß-Actin-normalized transcript abundance in the Pink1 KO samples versus that in the WT. Thus, in all cases, the WT values were considered=1.00. *P0.05 versus the corresponding WT (Student's t-test; n=3-4 independent experiments)."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(a) RT-qPCR analysis of Hif1α mRNA abundance in MEF transfected with siHif1α, or siControl (100 nM), after 72 h."
NCBI gene IDs:
"Hif1α: 15251"
Figure legend: "(b) siHif1α effectively knockdowns expressed Hif1α cDNA WT, but not a Hif1α cDNA silent mutant form refractory to siHif1α (Hif1α cDNA REFR.) in mice MEF."
NCBI gene IDs:
"Hif1α: 15251"
Figure legend: "(c) RT-qPCR analysis of the relative mRNA abundances of Glut1, Glut3, Hk2 and Gapdh in WT and Pink1 KO mice MEF transfected with siControl or siHif1α. ß-Actin mRNA was used for normalization, and all data are expressed as the change relative to WT-siControl."
NCBI gene IDs:
"ß-Actin: 
Gapdh: 407972
Hif1α: 15251
Hk2: 15277
Pink1: 68943
Glut1: 20525
Glut3: 20527"
Figure legend: "(d) Western blot against HIF1α in WT and in Pink1 KO mice MEF transfected with siHif1α or siControl shows the efficacy of the siHif1α at knocking down endogenous HIF1α; ß-actin was used as loading control. As shown, siHif1α partially prevented the increase in GLUT1, HK2 and GAPDH protein abundances."
NCBI gene IDs:
"Hif1α: 15251
Pink1: 68943"
Figure legend: "The increased rate of lactate released after 4 h of incubation (e) and glycolytic flux (f) observed in the Pink1 KO MEF was prevented by siHif1α."
NCBI gene IDs:
"Hif1α: 15251
Pink1: 68943"
Figure legend: "siHif1α was also effective at preventing the endogenous increased HIF1α protein (g), as well as the increased glycolytic flux (h) observed in Pink1 KO primary neurons. Only representative western blots are shown; the replicates and the semi-quantitative estimation of the band intensities are shown in the Supplementary Information."
NCBI gene IDs:
"Hif1α: 15251
Pink1: 68943"
Figure legend: "(a) Mitochondrial respiratory chain complexes activity values normalized to citrate synthase (CS), and (b) mitochondrial inner membrane potential (Δψm) in WT and Pink1 KO mice MEF."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(c) Western blot against PDK1 and HIF1α in WT and Pink1 KO MEF transfected with siHif1α (or its siControl); ß-actin was used as loading control."
NCBI gene IDs:
"HIF1α: 15251
Pink1: 68943"
Figure legend: "d) PDH complex activity in WT and Pink1 KO MEF transfected with siHif1α or siControl."
NCBI gene IDs:
"Hif1α: 15251
Pink1: 68943"
Figure legend: "Rate of whole-cell H2O2 (e) and mitochondrial O2·− (f) detection in WT and Pink1 KO MEF"
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(g) Detection of mitochondrial O2·− using the mitochondrial-specific probe MitoSox in MEF incubated in the absence (−) or in the presence (+) of the plasma membrane permeable GSH-EE, or by expressing mitochondrial-tagged forms of glutamate-cysteine ligase, (+mitoGCL; empty vector, pIRES2-EGFP, denoted as −) or catalase (+ mitoCAT; empty vector denoted as −), shows effective mitochondrial ROS removal."
NCBI gene IDs:
"catalase: 12359
mitoCAT: 12359
glutamate-cysteine ligase: 14629///14630
mitoGCL: 14630///14629"
Figure legend: "(h) Western blot against HIF1α in Pink1 KO MEF incubated with (or without) GSH-EE, or transfected with empty vector, mitoGCL or mitoCAT, showing reduction in HIF1α protein abundance by the treatments that remove mitochondrial ROS; α-TUBULIN was used as loading control. Only representative western blots are shown; the replicates and the semi-quantitative estimation of the band intensities are shown in the Supplementary Information. Data are expressed as mean±s.e.m. *P0.05 (Student's t-test; n=3-4 independent experiments)."
NCBI gene IDs:
"mitoCAT: 12359
mitoGCL: 14630///14629
Pink1: 68943"
Figure legend: "(a) Proportion of BrdU incorporation into DNA and cell cycle phases, and (b) cell growth in WT and Pink1 KO MEF after 72 h in the absence (denoted as -2DG) or in the presence (+2DG) of the glucose-metabolizing inhibitor, 2-deoxyglucose (2DG, 1 mM)."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(c) Proportions of BrdU incorporation into DNA and cell cycle phases, and (d) cell growth in WT and Pink1 KO MEF 72 h after transfection with siHif1α (+siHif1α) or siControl (denoted as -siHif1α)."
NCBI gene IDs:
"Hif1α: 15251
Pink1: 68943"
Figure legend: "(e) Ki67, Sox2 and DAPI immunostaining in the subventricular zone of WT and Pink1 KO mouse brain, and the corresponding quantification. *P0.05; #P0.05 versus all other conditions (analysis of variance followed by Bonferroni test; n=3-4 independent experiments). All data are expressed as mean±s.e.m. Scale bar, 100 μm."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "a) Rate of mitochondrial O2·- detection in WT and Pink1 KO mice primary neurons."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(b) Proportion of BrdU incorporation into DNA of WT and Pink1 KO neurons."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(c) Proportion of apoptosis in WT and Pink1 KO neurons, as assessed by the proportion of annexin V-positive/7-AAD-negative cells, determined by flow cytometry. Data are expressed as mean±s.e.m. *P0.05 (Student's t-test; n=3-4 independent experiments)."
NCBI gene IDs:
"Pink1: 68943"
Figure legend: "(A) Microcomputed tomography (micro-CT) images of the proximal femur  from 12-week-old male WT and Sigmar1  gKO mice. Scale bars, 1 mm. (B) Quantification of bone  volume per tissue volume (BV/TV), trabecular  number (Tb. N), trabecular  separation (Tb. Sp), trabecular  thickness (Tb. Th), cortical  region BV/TV (Ct. BV/TV) and cortical  thickness (Ct. Th, mm) (n = 5 biological replicates). Data information: All results are representative data generated from at least three independent experiments. Data are presented as mean ± SD. Unpaired two-tailed Student's t-test (B was used for statistical analysis."
NCBI gene IDs:
"Sigmar1: 18391"
Figure legend: "(E) TRAP staining  of tibias  from male WT and Sigmar1  gKO mice. Scale bars, 50 μm. (F and G) Quantification of osteoclast  number per bone  surface (N. Oc/BS) and percentage of osteoclast  surface per bone  surface (Oc. S/BS) (n = 6 biological replicates). Data information: All results are representative data generated from at least three independent experiments. Data are presented as mean ± SD. Unpaired two-tailed Student's t-test F-G, was used for statistical analysis."
NCBI gene IDs:
"Sigmar1: 18391"
Figure legend: "(A) Micro-CT images of the proximal femur from female WT or Sigmar1  gKO mice  that received sham  or ovariectomy   surgery for 6 weeks. Scale bars, 1 mm. (B) Quantification of bone  volume per tissue volume (BV/TV), trabecular  number (Tb. N), trabecular  separation (Tb. Sp) and trabecular  thickness (Tb. Th) (n = 5 biological replicates). Data information: All results are representative data generated from at least three independent experiments. Data are presented as mean ± SD. One-way ANOVA with Tukey's multiple comparisons test (B were used for statistical analysis."
NCBI gene IDs:
"Sigmar1: 18391"
Figure legend: "(F) TRAP staining  to detect osteoclastogenesis  of BMMs  from WT or Sigmar1  gKO mice. Scale bars, 200 μm. (G and H) Quantification of the size and nuclei  numbers of TRAP-positive multinuclear cells (n = 6 biological replicates for G and n = 3 biological replicates for H). Data information: All results are representative data generated from at least three independent experiments. Data are presented as mean ± SD. One-way ANOVA with unpaired two-tailed Student's t-test (G-H were used for statistical analysis."
NCBI gene IDs:
"Sigmar1: 18391"
Figure legend: "(I and J) Representative images and quantification of the relative pit  resorption    area of hydroxyapatite-coated plates. WT or Sigamr1  gKO BMMs  were seeded on hydroxyapatite-coated plates and treated with 50 ng/mL RANKL  (n = 6 biological replicates). Data information: All results are representative data generated from at least three independent experiments. Data are presented as mean ± SD. One-way ANOVA with unpaired two-tailed Student's t-test J) were used for statistical analysis."
NCBI gene IDs:
"Sigamr1: 18391"
Figure legend: "(D) Western blots showing SERCA2  expression  in HEK-293T  cells transfected with different amounts of Sigmar1  plasmids. The cells were transfected with 1 μg SERCA2  plasmid and Sigmar1  plasmid (0.125 μg, 0.25 μg, 0.5 μg, 1 μg). Data information: All results are representative data generated from at least three independent experiments."
NCBI gene IDs:
"SERCA2: 11938
Sigmar1: 10280"