EMBO/SourceData huggingface dataset:
Figure legend: "Clonogenic survival assays of U2OS WT and PHF6 knockout cells that were depleted for luciferase or XRCC4 and treated with 2, 3 or 4 Gy IR. Shown is the relative survival as compared to the undamaged control. Error bars represent the SEM of three individual experiments."
NCBI gene IDs:
"luciferase: 
PHF6: 84295
XRCC4: 7518"
Figure legend: "GC92 SV40 immortalized human fibroblasts containing the I-SceI NHEJ reporter were transfected with the indicated siRNA oligos and I-SceI. 48 h later genomic DNA was extracted and repair of I-SceI cut-sites analyzed through junction analysis (n= independently derived sequences, see materials and methods for details)."
NCBI gene IDs:
"I-SceI: 854590"
Figure legend: " (I) RT-qPCR of ZIKV RNA in infected hNSCs transfected with siRNAs targeting LC3, BECN or ATG9A 48 h post infection. Mean ± SEM of 3 biological replicates. *p &lt; 0.05 by Student's t test. "
NCBI gene IDs:
"ATG9A: 79065
BECN: 8678
LC3: 81631"
Figure legend: "(C-D) RT-qPCR analysis of ZIKV mRNA 48 h after ZIKV infection of HeLa cells overexpressing FANCC (C) or FANCC-specific siRNA (D). Mean ± SEM of biological triplicates. **p &lt; 0.01 by Student's t test."
NCBI gene IDs:
"FANCC: 2176"
Figure legend: "(E) Flow cytometry analysis of ZIKVE expression in mock-infected (red), ZIKV-infected (blue), ZIKV-infected siFANCC-transfected (orange), and ZIKV-infected siE2F4-transfected (green) hNSCs at 48 h post-infection."
NCBI gene IDs:
"E2F4: 1874
FANCC: 2176"
Figure legend: "(G) ZIKV NS1 expression 48 h after ZIKV infection of hNSCs expressing control siRNA (NTC) or siFANCC-targeting siRNA. Scale bar = 100 µm. (H-I) ZIKV NS1 expression 48 h after ZIKV infection of hNSCs expressing control (NTC) or siE2F4-targeting siRNA. Mean ± SEM of six imaging fields of biological triplicates, **p &lt; 0.01 by Student's t test, Scale bar = 100 µm, "
NCBI gene IDs:
"E2F4: 1874
FANCC: 2176"
Figure legend: "(J) Immunoblot of macroautophagy (p62, LC3I/LC3II) and selective autophagy (FANCC, TOMM20) proteins 24 h after overexpression of FLAG-tagged ZIKV NS1, NS2B, NS3, NS4A, NS4B and NS5 in 293FT cells."
NCBI gene IDs:
"FLAG: 
NS1: 7751225
NS2B: 7751225
NS3: 7751225
NS4A: 7751225
NS4B: 7751225
NS5: 7751225"
Figure legend: "(L-M) Heatmaps of RT-qPCR analysis of FA pathway genes (K) or essential selective autophagy genes (L) in hNSCs 48 h after transfection with control (NTC) or E2F4-targeting siRNAs or after infection with ZIKV MR766 and Paraiba strains. Color patterns in heat map indicate as red showing the highest expression and blue showing the lowest expression of genes."
NCBI gene IDs:
"E2F4: 1874"
Figure legend: "(A) Representative images of mock- or ZIKV-infected human neurospheres expressing control (NTC) or FANCC-targeting siRNAs at 1-4 days post-infection. Scale bar = 250 µm. (B-C) Violin plot (B) and box plot (C) of relative size of neurospheres treated as described in (A) and measured on day 4 (B) or days 1-4 (C). Box plots show the mean and the smallest; largest values in whiskers represent the 10th and 90th percentiles in 50 randomly selected cells per group; and solid horizontal line indicates median. ***p &lt; 0.001 by Student's t test. "
NCBI gene IDs:
"FANCC: 2176"
Figure legend: "Immunostaining of neural progenitor marker SOX2 (green) and ZIKV envelope flavivirus group antigen (ZIKVE, red) in the hippocampus of uninfected or ZIKV Paraiba-infected Ifnar-/- mice six days post-infection. Nuclei were stained with DAPI (gray). Right-most column in (A) shows enlargements of the regions. CC = cortical cortex, GCL = granular cell layer, LV = lateral ventricle, SGZ = subgranular zone, STR = striatum, SVZ = subventricular zone. Scale bars, 100 μm."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "Immunostaining of neural progenitor marker SOX2 (green) and ZIKV envelope flavivirus group antigen (ZIKVE, red) in the SVZ of uninfected or ZIKV Paraiba-infected Ifnar-/- mice six days post-infection. Nuclei were stained with DAPI (gray). Right-most column in (A) shows enlargements of the regions. CC = cortical cortex, GCL = granular cell layer, LV = lateral ventricle, SGZ = subgranular zone, STR = striatum, SVZ = subventricular zone. Scale bars, 100 μm."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "(C) RT-qPCR analysis of relative ZIKV RNA in different brain regions of Ifnar-/- mice. mean±SEM of n=3 biological replicates, **p&lt;0.005, ***p&lt;0.001 by Student's t test."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "(D) RT-qPCR analysis of relative Fancc mRNA levels from whole brains or testes in mock- and ZIKV Paraiba infected Ifnar-/- mice six days post-infection. Mean ± SEM of n=3 biological replicates, *p &lt; 0.05 and ns by Student's t test."
NCBI gene IDs:
"Fancc: 14088
Ifnar: 15975"
Figure legend: "(E) RT-qPCR analysis of relative Fancc or Fancl mRNA levels from whole brains from mock- and ZIKV Paraiba infected Ifnar-/- newborn mice. Mean ± SEM of n=3 biological replicates, ***p &lt; 0.001 by Student's t test."
NCBI gene IDs:
"Fancc: 14088
Fancl: 67030
Ifnar: 15975"
Figure legend: "(F) Western blot analysis of Fancc protein in brain regions from mock- and ZIKV Paraiba infected Ifnar-/- mice six days post-infection. HP = hippocampus, CT= cortex, ST = striatum and OB= Olfactory Bulb."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "(H) RT-qPCR analysis of relative ZIKV RNA in different brain regions of Fancc KO neonatal mice six days post-infection. mean±SEM of n=3 biological replicates, **p&lt;0.005, ***p&lt;0.001 by Student's t test."
NCBI gene IDs:
"Fancc: 14088"
Figure legend: "(I) qRT-PCR for Ifnab gene expression in wild type and Fancc KO mice, Mean ± SEM of n=3 biological replicates, ns by Student's t test."
NCBI gene IDs:
"Fancc: 14088
Ifnab: 15974"
Figure legend: "(A) Immunostaining of immature neuronal marker DCX (green) and ZIKV envelope (ZIKVE, red) in the hippocampus of uninfected or ZIKV Paraiba-infected Ifnar-/- mice six days post-infection. Nuclei were stained with DAPI (gray). Bottom row shows enlargements of the hippocampus region. GCL = granular cell layer, SGZ = subgranular zone, DG = dentate gyrus, InGr = internal granular layer, Me = medulla, OB = olfactory bulb. Scale bars, 50 and 100 μm."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "Immunostaining of immature neuronal marker DCX (green) and ZIKV envelope (ZIKVE, red) in the olfactory bulb of uninfected or ZIKV Paraiba-infected Ifnar-/- mice six days post-infection. Nuclei were stained with DAPI (gray). InGr = internal granular layer, Me = medulla, OB = olfactory bulb. Scale bars, 50 and 100 μm."
NCBI gene IDs:
"Ifnar: 15975"
Figure legend: "(C-E) Western blot of autophagy protein LC3 and p62 in different brain region of Fancc KO neonatal mice six days post-infection of ZIKV (MR766). Mean ± SEM of n=3 biological replicates, *p&lt;0.05, **p&lt;0.005, by Student's t test. HP = hippocampus, CT= cortex, ST = striatum and OB= Olfactory Bulb."
NCBI gene IDs:
"Fancc: 14088"
Figure legend: "(F) Representative immunofluorescent images showing immunoreactivity of neural progenitor marker (Sox2) with apoptosis marker (Cleaved-caspase-3, CC3) in hippocampus region of Fancc KO mice. Scale bar=50µm. (G) Quantitative analysis of number of Sox2+ cells and Sox2/CC3+ co-labelled cells in Fancc KO mice hippocampal brain region. Mean ± SEM, n=3 biological replicates, *p &lt; 0.05 by Student's t test.  "
NCBI gene IDs:
"Fancc: 14088"
Figure legend: "A, B Nucleosome maps at and surrounding, the TSS region of the NANOG and GRIA1 genes in pl-iPSC (upper, black) and in NPC cells (lower, grey). The TSS is indicated by a dashed line at chr 12: 7,941,991 for NANOG and chr5: 152,870,105 for GRIA1. * indicates the position of a highly positioned nucleosome associated with the TSS of the active gene."
NCBI gene IDs:
"GRIA1: 2890
NANOG: 79923"
Figure legend: "(E-F) Serum levels of T (E) and LH (F) in WT males, ARLmon/Y and AR-/Y mice at the age of 13 weeks."
NCBI gene IDs:
"AR: 11835"
Figure legend: "(D) RT-qPCR analyses of Rhox5 and Insl3 of testes from 13-week old WT males, ARLmon/Y and AR-/Y. Expression levels are normalized to WT."
NCBI gene IDs:
"AR: 11835
Insl3: 16336
Rhox5: 18617"
Figure legend: "(B) Serum levels of A-dione in WT males, ARLmon/Y and AR-/Y mice at the age of 13 weeks."
NCBI gene IDs:
"AR: 11835"
Figure legend: "(D) Reporter gene assays in Hela cells co-transfected with the mouse Insl3 promotor together with the WT or mutant mouse AR."
NCBI gene IDs:
"AR: 11835
Insl3: 16336"
Figure legend: "mRNA expression levels extracted from RNA-seq data for Fkbp5 (D) in kidneys used for RNA-seq. Expression levels are normalized to WT ORX."
NCBI gene IDs:
"Fkbp5: 14229"
Figure legend: "mRNA expression levels extracted from RNA-seq data for , Odc1 (E) and Kap (F) in kidneys used for RNA-seq. Expression levels are normalized to WT ORX."
NCBI gene IDs:
"Kap: 16483
Odc1: 18263"
Figure legend: "(C) Western blot on cytoplasmic, nuclear and whole cell extracts derived from Hela cells transfected with human WT or W752R AR followed by stimulation with increasing concentrations of DHT. Both panels represent the same blot. The blot was cut in two parts and antibodies against HSP90 and Lamin A/C were used to confirm cellular fractionation of cytoplasmic and nuclear proteins. For AR visualization (upper panel), longer exposure time was used. # = residual AR expression (lower panel)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(B) AR occupancy (blue) and levels of the histone marks H3K27ac (green) and H3K27me3 (red) at ARBS within the regulatory regions of Fkbp5, Kap (both androgen-regulated in kidney) and Tox3 (androgen-regulated in prostate but not in kidney and hence used as a negative control) were assessed by ChIP-qPCR in kidneys from 13-week-old ARLmon/Y mice and WT males."
NCBI gene IDs:
"AR: 11835
Fkbp5: 14229
Kap: 16483
Tox3: 244579"
Figure legend: "(C) Representative pictures of immunofluorescence AR staining (green) in kidneys of castrated WT males and ARLmon/Y mice supplemented with vehicle or T. Nuclei are shown in blue."
NCBI gene IDs:
"AR: 11835"
Figure legend: "(B) Confocal fluorescence microscopy images of AR-WT-BirA* and AR W752R-BirA*expressing HEK293 cells treated in the presence of 50 µM biotin with 100 nM DHT or vehicle (ethanol) and with or without 0.03 µg/ml TET as indicated. AR-BirA*s were detected with anti-AR (red) and biotinylated proteins with fluorescently labeled streptavidin (green). Nuclei were visualized using DAPI."
NCBI gene IDs:
"AR: 367
BirA: 948469"
Figure legend: "Yeast pat1Δ mutants are unable to grow at 37°C while wild‐type (B4742) strain grows at 30°C (left panel) and 37°C (right). Growth at 37°C is restored in pat1Δ expressing AtPAT1. Serial dilutions of each yeast strain were plated on YPAD agar plates and grown at the indicated temperatures."
NCBI gene IDs:
"pat1: 850440
PAT1: 834867"
Figure legend: "Agarose gel electrophoresis of 5′ RACE PCR to detect capped transcripts of EIF4A1, UGT87A2 and EXPL1 in 14‐day‐old Col‐0 and pat1‐1."
NCBI gene IDs:
"UGT87A2: 817566
EIF4A1: 820605
EXPL1: 823740
pat1: 850440"
Figure legend: "pat1 mutants have a serrated leaf, semi‐dwarf phenotypePlants photographed at 4 weeks of growth in short day conditions, genotypes as indicated. Col‐0 and eds1‐2 plants and pat1‐1 and pat1‐1/eds1‐2, respectively, were placed in the middle of the same pots before the pictures were taken."
NCBI gene IDs:
"eds1: 
pat1: 850440"
Figure legend: "PR gene expression is elevated in mpk4‐2 and pat1‐1 mutants. Four‐week‐old plants were used for RNA extraction, followed by qRT‐PCR. Fold‐change in PR1 (gray bars) and PR2 (black) expression is relative to Col‐0, normalized to UBQ10. Standard error of the mean is indicated by errors bars (n = 3). Statistical significance between the mean values was determined by ANOVA followed by Fisher's LSD test, P‐values are only shown for mean values significantly different from Col‐0."
NCBI gene IDs:
"mpk4: 
PR1: 
PR2: 
UBQ10: 
pat1: 850440"
Figure legend: "pat1 is more resistant to colonization by P. syringae pv. tomato DC3000. Bacteria were syringe‐infiltrated and samples taken 3 days post‐infiltration. Data are shown as log10‐transformed colony‐forming units/cm2 leaf tissue (cfu/cm2). Standard error of the mean is indicated by errors bars (n = 4). Statistical significance between the mean values was determined by ANOVA followed by Fisher′s LSD test."
NCBI gene IDs:
"pat1: 850440"
Figure legend: "PAT1 protein is induced by PAMP treatment. Immunoblot detection of equal amounts of protein from Col‐0 seedlings at times in minutes as indicated following vacuum infiltration with 1 μM flg22 or water (180 min after infiltration). Immunoblots were probed with anti‐PAT1 antibodies. Negative control pat1‐1 was loaded for comparison. Coomassie brilliant blue protein loading control is indicated by CBB."
NCBI gene IDs:
"pat1: 850440"
Figure legend: "Elevated PR gene expression in pat1 mutants is suppressed by summ2‐8. Four‐week‐old plants were used for RNA extraction, followed by qRT‐PCR. Fold‐change in PR1 (gray bars) and PR2 (black bars) expression is relative to Col‐0, normalized to UBQ10. Standard error of the mean is indicated by errors bars (n = 3)."
NCBI gene IDs:
"PR1: 
PR2: 
summ2: 
UBQ10: 
pat1: 850440"
Figure legend: "pat1 resistance to P. syringae pv. tomato DC3000 is suppressed by summ2‐8. Bacteria were syringe‐infiltrated and samples taken 3 days post‐infiltration. Data are log10‐transformed colony‐forming units/cm2 leaf tissue (cfu/cm2). Standard error of the mean is indicated by errors bars (n = 4)."
NCBI gene IDs:
"summ2: 
pat1: 850440"
Figure legend: "14‐day‐old seedlings grown on ms plates were used for RNA extraction. Next, 1 μg of RNA from each sample was treated with XRN1 (NEB) or mock treated before RT‐qPCR. Data were normalized to ACT2, and transcript levels were compared between XRN1 and mock‐treated RNA for each genotype. Standard error of the mean is indicated by error bars (n = 4). Statistical significance between the mean values was determined by ANOVA followed by Fisher's LSD test."
NCBI gene IDs:
"ACT2: 
XRN1: "
Figure legend: "Phosphorylation of the PAT1 peptide SSFVSYPPPGSISPDQR, which include Ser208, in Col‐0 and mkk1/1‐summ2 before and after flg22 treatment. The phosphorylation stoichiometry is illustrated relative to Col PAT1 without flg22 treatment. The ratios were obtained using quantitative iTRAQ mass spectrometry."
NCBI gene IDs:
"mkk1: 
summ2: "
Figure legend: "(B) THP-1 cells were subjected to GABARAP knockdown and processed as in A."
NCBI gene IDs:
"GABARAP: 11337"
Figure legend: "(G) In vitro translated and radiolabeled [35S] myc-HA-TRIM16 was incubated with GST-galectin-8 in the presence (+) or absence (-) of flag-ULK1 and cold ATP, GST pulldowns were performed, and [35S] radiolabeled Myc-HA-TRIM16 in pulled-down material detected by PAGE and autoradiography. Amounts of GST fusion proteins are shown in coommassie brilliant blue (CBB)-stained gels. Data, means  SEM; n≥5, except for immunoblot quantifications where n≥3. *P &lt; 0.05, †P ≥ 0.05 (t test or ANOVA)."
NCBI gene IDs:
"ULK1: 8408"
Figure legend: "(C) IL-1β levels in 25k membrane fractions (pellets) from HeLa cells and their CRISPR knockout derivatives (TRIM16KO) that were reconstituted for IL-1β secretion and treated with 1mM LLOMe for 1 h."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(F,G) Sucrose density gradient analysis of 25k membranes. Note that mature IL-1β (mIL-1β) is recruited to and co-fractionates with TRIM16+, Sec22b+, and LC3-II+ membranes in TRIM16wt HeLa cells reconstituted with pro-IL-1β and pro-caspase 1, but not in TRIM16KO mutant cells."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(L) Co-IP analysis of interactions between deletion variants of flag-TRIM16 with GFP-Sec22b in HEK293T cells."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(M and N) IL-1β secretion complementation experiments of TRIM16KO cells with TRIM16 Sec22b-interacting and its Sec22b-nonbinding mutant shown."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "B ChIP gel images showing the relative enrichment by H3K4me3 in controls (n=9) and PD patients (n= 18). PCR amplified a 188-bp region of SNCA from intron 1 where H3K4me3 peak was at its optimum. Mouse IgG (mIgG) was used as control and the bands were normalized by unbiased amplification from respective inputs. Data information: Data represent mean ± standard error of the mean."
NCBI gene IDs:
"SNCA: 6622"
Figure legend: "D The graph represents the statistically significant difference of relative H3K4me3 enrichment between PD (n=7) and controls (n=6). Neuronal nuclei from PD brain samples show significantly higher enrichment of H3K4me3 at SNCA intron 1 (p=0.01) compared to controls. *P &lt; 0.05. Data were analyzed using non-parametric t-test followed by Mann-Whitney post-hoc corrections. Two-tailed p-values were calculated for all. Data information: Data represent mean ± standard error of the mean."
NCBI gene IDs:
"SNCA: 6622"
Figure legend: "The corresponding α-synuclein levels C) were evaluated in SH-SY5Y cells expressing dCas9-5xGCN4 with (sgA-dCas9) or without scFV-sfGFP-JARID1A (sgA-dCas9-JA). C α-SYN levels in SH-SY5Y cells were also evaluated using western blot. The levels of α-SYN was normalized to β-actin. Significant reduction of α-SYN was observed in cells expressing JARID1A. Three independent repeats were performed. Percentage of reduction is shown in the graph. **P &lt;0.01. Data were analyzed using non-parametric t-test followed by Mann-Whitney post-hoc corrections. Two-tailed p-values were calculated. Data information: All data are presented as mean ± SEM."
NCBI gene IDs:
"Cas9: 
GFP: 
GCN4: 856709
JA: 5927
JARID1A: 5927"
Figure legend: "E ChIP on H3K4me3 at the SNCA promoter between sPD1-1 lines after locus-specific epigenomic modulation. Differentiated cells were transiently transfected with either sgA-dCas9 5xGCN4-scFV-JARID1A or sg A-dCas9 5xGCN4-scFV-empty backbone vectors. A significant reduction in H3K4me3 at the SNCA promoter was observed in cells transfected with JARID1A. Data information: Three independent repeats were performed for both ChIP experiments. *P &lt; 0.05; ****P&lt;0.0001. Data were analyzed using non-parametric t-test followed by Mann-Whitney post-hoc corrections. One-tailed p-values were calculated for ChIP analysis"
NCBI gene IDs:
"Cas9: 
GCN4: 856709
JARID1A: 5927
SNCA: 6622"
Figure legend: "F Western blot analysis of α-synuclein (α-SYN) levels in the cells under the same conditions as analyzed in (E). A significant decrease (56-66%) in α-SYN levels was observed in cells transfected with JARID1A. The normalized and relative expression of α-SYN in JARID1A transfected cells are shown as a percentage of control (transfected by empty backbone vector). Data information: Three independent repeats were performed for western blot experiments. *P &lt; 0.05; ****P&lt;0.0001. Data were analyzed using non-parametric t-test followed by Mann-Whitney post-hoc corrections. two-tailed p-value was calculated for western blot experiments. All data are presented as mean ± SEM."
NCBI gene IDs:
"JARID1A: 5927"
Figure legend: "F NALCN  knockdown suppresses invadopodia  formation reported by Cy3-fluorescent gelatin  degradation (arrows). Confocal  images (left) of Cy3  (red) and DAPI  (blue; nuclei) fluorescence in control (shCTL) and shNALCN  48 hours after plating the cells on gelatin. Bar diagram plot: NALCN  suppression causes significant decrease of the number of gelatin  degradation puncta counted within 48 h of incubation. Data information: The images: scale bar is 100 µm. Bar diagram plots show the degradation puncta per cell (F) mean±S.E.M. for N=5 (F) biological replicates per condition. **P&lt;0.01, ***P&lt;0.001, unpaired two-tailed Student's t-test."
NCBI gene IDs:
"NALCN: 259232"
Figure legend: "C-D [Ca2+]c oscillations reported by  Fluo-4  fluorescence in FBS-exposed  PC-3 cells pre-treated with siCTL (C) or siNALCN (D) for 72 hours. Top: sample traces from 8 different cells. Middle: POIs (top: grey background) are presented on enlarged plots to emphasize that NALCN  suppression abolishes \"pacemaker events\" observed in control (fitted red curves). Bottom: the galleries show every 3rd image during the POIs (middle: orange background). Scale bars: 10 μm."
NCBI gene IDs:
"NALCN: 259232"
Figure legend: "A-D Cytosolic Na+ concentration ([Na+]c) changes reported by ratiometric Na+  indicator SBFI  following (A-C) switch of extracellular Na+ concentration ([Na+]o) from 0 to 130 mM or (D) application of 1 µM thapsigargin  (TG). The effects of: (A) NALCN  knockdown, (B) extracellular Ca2+ concentration ([Ca2+]o) and (C) Ca2+  store depletion. Data information: Data show mean±S.E.M. The cartoons highlight experimental design and the plots show mean±S.E.M. traces (n=45-501) of SBFI  (olive) and Fura-2 (wine) fluorescence with the axes (∆R/R0) presented in corresponding colour."
NCBI gene IDs:
"NALCN: 259232"
Figure legend: "E-H Store-operated Ca2+  entry (SOCE)-induced changes of [Na+]c (SBFI) and [Ca2+]c (Fura-2). The effects of: NALCN  knockdown (E), the reverse-mode plasmalemmal Na+/Ca2+ exchanger (RM-NCX) inhibitor KB-R7943 (1 µM) either at [Na+]o=130 mM (F) or upon [Na+]o switch from 0 to 130 mM following SOCE activation (G), and knockdown of the mitochondrial Na+/Ca2+ exchanger, siNCLX (H). Data information: Data show mean±S.E.M. The cartoons highlight experimental design and the plots show mean±S.E.M. traces (n=45-501) of SBFI  (olive) and Fura-2 (wine) fluorescence with the axes (∆R/R0) presented in corresponding colour."
NCBI gene IDs:
"NALCN: 259232
NCLX: 80024"
Figure legend: "I SOCE augments NALCN-mediated Na+ influx  (top) by addressing NALCN  to plasma membrane (bottom). Data information: Plots (I, top) compare mean rates of Na+ influx  induced by [Na+]o-switch (n=283-360) and SOCE (n=180-216), and their shNALCN-sensitive fractions. Immunoblotting  (I, bottom) compares NALCN expression  in total cell lysates and biotinylated fractions before and after SOCE induction. Numbers show (in corresponding colour) mean values (n=3) of NALCN  protein levels (band intensity) normalized to N-Cadherin, Calnexin  and β-actin."
NCBI gene IDs:
"NALCN: 259232"
Figure legend: "D Suppression of NCLX  with siNCLX attenuates [Ca2+]c oscillations (Fluo-4). Data information: In D, mean signal temporal densities during highlighted periods were compared in control (n=18) and following 48 h pre-treatment with siNCLX (n=17). Bar diagram plots: mean±S.E.M. n= number of cell, ***P&lt;0.001, unpaired two-tailed Student's t-test. Cartoons (left) highlight experimental design and assessed signaling pathways. Note that Na+ delivered to the cytosol  by NALCN, on the one hand, activates RM-NCX, while on the other hand, is exchanged by NCLX  to Ca2+ thus restricting elevation of [Ca2+]mito and, hence, production of superoxide  (O2-) and H2O2, which is known to inhibit SERCA, RM-NCX and SOCE elements."
NCBI gene IDs:
"NALCN: 259232
NCLX: 80024"
Figure legend: "E Plot: mean rates of secretion calculated as signal mass (cyan, left) per second. Data information: Data (E): mean±S.E.M. for siCTL (n=35), siNALCN (n=40), Control (n=45), EGTA-AM  (n=35) and BAPTA-AM  (n=62). n= number of cell, ***P&lt;0.001; and n.s., not significant, unpaired two-tailed Student's t-test."
NCBI gene IDs:
"NALCN: 259232"
Figure legend: "D Left: Dorsal (top) and ventral (bottom) images of bioluminescence from mice  31 days after intra-tibial  injection of control (shCTL: PC-3 Luc-shCTL) or NALCN  depleted cells (shCTL: PC-3 Luc-shNALCN). Right: statistical comparison of total photon fluxes between the two groups of 10 mice  (c.p.s.: count of photons per second±SD). Data information: *P&lt;0.05, **P&lt;0.01, and n.s., not significant, two-tailed Mann-Whitney U test."
NCBI gene IDs:
"Luc: 
NALCN: 259232"