EMBO/SourceData huggingface dataset:
Figure legend: "F. Normal replication tract length, quantified using the DNA fiber assay, is restored by expressing wild-type, but not PIP-mutant HUWE1, in the HUWE1-knockout 293T cells. The \"n\" numbers of fibers analyzed (pooled from three independent experiments), as well as the mean +/- SEM, are indicated on the graphs. If not indicated otherwise, the p-values shown specify the statistical significance relative to 293T. P-values are: 1.47x10-11 (293T vs HUWE-/- samples), 0.094 (293T vs HUWE-/- +WT samples), 1.40x10-7 (293T vs HUWE-/- +FF samples), 1.22x10-7 (HUWE-/- vs HUWE-/- +WT samples), 0.679 (HUWE-/- vs HUWE-/- +FF samples)."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: "G. Alkaline Comet Assay showing that wild-type, but not the FF mutant can correct the breakage phenotype of HUWE1-knockout 293T cells. The \"n\" numbers of comet tails analyzed (pooled from three independent experiments), as well as the mean +/- SEM, are indicated on the graphs. The p-values indicated are: 4.18x10-43 (293T vs HUWE-/- samples), 6.89x10-41 (HUWE1-/- +WT vs FF samples), and 5.97x10-50 (HUWE1-/- +WT vs C4341A samples)."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: "A, B. The impact of HUWE1 on H2AX phosphorylation and ubiquitination, under normal conditions (A) or upon induction of replication stress (2mM HU for 18h, or 2h after exposure to 40J/m2 UV). Control or HUWE1-knockout HeLa cells were analyzed."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: "C. In vitro ubiquitination assay showing that recombinant HUWE1C-ter can mono-ubiquitinate H2AX."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: "D. Wild-type, but not the PIP-mutant can correct the defective H2AX ubiquitination in HUWE1-knockout 293T cells."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: ". E. Chromatin immunoprecipitation showing that H2AX binding to the common fragile site FRA3B is reduced in HUWE1-knockout 8988T cells. Cells were treated with 600nM aphidicolin for 24h. Binding was quantified relative to input material. Shown is the average of 4 experiments -/+ SD. P-value is 4.4x10-6."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: "F. Western blots showing that several H2AX ubiquitin ligases participate in H2AX ubiquitination following replication stress. HUWE1, RNF168, and BMI1 were knocked-down in HeLa cells. The efficiency of the knockdown is shown in Fig EV4. Cells were treated with 600nM aphidicolin for 24h, 2mM HU for 24h, or analyzed 2h after exposure to 40J/m2 UV."
NCBI gene IDs:
"BMI1: 648
HUWE1: 10075
RNF168: 165918"
Figure legend: "H. Chromatin fractionation experiments showing that HUWE1-knockout HeLa cells, treated with 2mM HU for 24h, fail to efficiently recruit BRCA1 and BRCA2 proteins to DNA. In contrast, 53BP1 chromatin association is not induced by HU treatment, and not affected by HUWE1 knockout, and thus can serve as loading control. Shown are blots of whole cell extract (WCE) samples, representing input material, and of chromatin pellet samples."
NCBI gene IDs:
"HUWE1: 10075"
Figure legend: " Whole cell lysates from HEK293T cells, with or without stable expression of ATF6α (as indicated) were immunoisolated with mouse anti-V5. RCN1 was transiently transfected into both cell lines in the experiment depicted in panel 3. Immunoisolated material was separated by SDS-PAGE under reducing conditions followed by western blotting to determine the co-isolation of ER proteins with ATF6α. Ig-HC (anti-BiP blot) indicates immunoglobulin heavy chains."
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " Whole cell lysates from HEK293T cells stably expressing ATF6α were either untreated (-) or treated (+) with 5 µM thapsigargin (TG) for 1 h. THBS4 was transiently transfected into cells in the experiment depicted in panel 2. ATF6α was either immunoisolated with anti-V5 (panel 1 and 3) or anti-HA (panel 2). For the experiment in panel 3 the cells were cross-linked prior to immunoisolation. Samples were separated by SDS-PAGE under reducing conditions followed by western blots to determine co-isolation of endogenous PDIR, ERp18 or exogenously expressed THBS4 with ATF6α. HC indicates immunoglobulin heavy chain. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: "HEK293T cells stably expressing ATF6α were either left untransfected (UT) or transfected with substrate-trapping mutants of PDI oxidoreductases. ATF6α was immunoisolated from cell lysates with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions and ATF6α detected by western blotting using rabbit anti-HA. Blots confirm mixed-disulfide complexes between ATF6α and ERp18, ERp57, and PDIR indicated with arrows (lanes 2, 4, 5). M, D and O refer to ATF6α monomer, dimer and oligomer respectively."
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " Whole cell lysates of HEK293T cells expressing an ATF6α mutant containing cysteine to alanine mutations in the lumenal domain (ATF6 DM) were transfected with PDI substrate-trapping mutants. ATF6α was immunoisolated with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions, and ATF6α detected with rabbit anti-HA. "
NCBI gene IDs:
"ATF6: 22926
ATF6α: 22926"
Figure legend: " HEK293T cells were co-transfected with ATF6α S1P and PDI substrate-trapping mutants and either left untreated (-) or treated (+) for 1 h with 5 µM TG to induce ER stress. ATF6α was immunoisolated from cell lysates with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions and ATF6α detected by western blotting using rabbit anti-HA. Blots indicate that mixed-disulfide complexes between ATF6α and PDI enzymes are regulated by ER stress. M, D and O refer to ATF6α monomer, dimer and oligomer respectively. *indicates positions of the ATF6α-PDI enzymes mixed-disulfide complexes. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " HEK293T cells were co-transfected with ATF6α S1P and PDI substrate-trapping mutants and either left untreated (-) or treated (+) for 1 h with 5 µg/ml tunicamycin to induce ER stress. ATF6α was immunoisolated from cell lysates with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions and ATF6α detected by western blotting using rabbit anti-HA. Blots indicate that mixed-disulfide complexes between ATF6α and PDI enzymes are regulated by ER stress. M, D and O refer to ATF6α monomer, dimer and oligomer respectively. *indicates positions of the ATF6α-PDI enzymes mixed-disulfide complexes. ** indicates hypoglycosylated ATF6α. Data shown are representative of three independent experiments. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " HEK293T cells were co-transfected with ATF6α S1P and PDI substrate-trapping mutants and either left untreated (-) or treated (+) for 1 h with 20 µM MG132 to induce ER stress. ATF6α was immunoisolated from cell lysates with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions and ATF6α detected by western blotting using rabbit anti-HA. Blots indicate that mixed-disulfide complexes between ATF6α and PDI enzymes are regulated by ER stress. M, D and O refer to ATF6α monomer, dimer and oligomer respectively. *indicates positions of the ATF6α-PDI enzymes mixed-disulfide complexes. ** indicates hypoglycosylated ATF6α. Data shown are representative of three independent experiments. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " HEK293T cells were co-transfected with ATF6α S1P and PDI substrate-trapping mutants and either left untreated (-) or treated (+) for 1 h with 5 µM TG to induce ER stress. Samples from (A) were rerun and probed with anti-sera raised against respective PDI enzymes as indicated (D). *indicates positions of the ATF6α-PDI enzymes mixed-disulfide complexes. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " HEK293T cells were co-transfected with the ERp18 substrate-trapping mutant and the indicated ATF6α constructs. Cell were allowed to recover for 24 h post-transfection before being either left untreated (-) or treated (+) for 1 h with 5 µM TG to induce ER stress. ATF6α was immunoisolated from cell lysates with mouse anti-ATF6α, separated by SDS-PAGE under non-reducing conditions and ATF6α detected by western blotting using rabbit anti-HA. The blot confirms that ATF6α C618A forms a stable complex with ERp18 trapping mutant following ER stress. M, D and O refer to ATF6 monomer, dimer and oligomer respectively. *indicates positions of the ATF6-ERp18 mixed-disulfide complexes. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " HEK293T cells were co-transfected with GFP-ATF6α and ERp18 trapping mutant constructs as indicated. GFP-ATF6α complexes were immunoisolated using GFP-trap. Immunoisolated material was separated by SDS-PAGE under non-reducing conditions and silver stained to detect ATF6α disulfide-linked complexes. MS analyses confirmed that protein bands indicated by black arrows are a complex comprising ATF6α and ERp18, whilst bands marked as monomer (M) and oligomer (O) contain ATF6α only. "
NCBI gene IDs:
"GFP: 
ATF6α: 22926
ERp18: 51060"
Figure legend: "CRISPR/Cas9-based knockout of ERp18 in HEK293T cells expressing ATF6α. ERp18 levels in control (WT) and knockout (KO) cells were detected with an antibody to ERp18."
NCBI gene IDs:
"Cas9: 
CRISPR: 
ATF6α: 22926
ERp18: 51060"
Figure legend: " qPCR analysis of UPR target genes in the absence of ER stress. Wild type and ERp18 knockout HEK293T cells were untreated prior to isolation of mRNA for qPCR analysis. mRNA levels for Grp78, Grp94, ATF6α, ATF4, XBP1s (spliced form of XBP1) and ERp72 were normalized to GAPDH and then ERp18 KO levels compared to wild type. Error bars represent ± standard deviation for three independent experiments.   qPCR analysis of UPR target genes following ER stress. Wild type and ERp18 knockout HEK293T cells were treated with 1 µM TG for approximately 16 h prior to isolation of mRNA for qPCR analysis. mRNA levels for Grp78, Grp94, ATF6α, ATF4, XBP1s (spliced form of XBP1) and ERp72 were normalized to GAPDH and then ERp18 KO levels compared to wild type controls. Error bars represent ± standard deviation for three independent experiments.   "
NCBI gene IDs:
"ATF4: 468
ATF6α: 22926
GAPDH: 2597
Grp94: 7184
Grp78: 3309
ERp72: 9601
ERp18: 51060
XBP1: 7494
XBP1s: 7494"
Figure legend: " Levels of BiP protein in wild type or ERp18 KO cells before and after ER stress induction with thapsigargin (TG). Panel 1 compares wild type and ERp18 KO cells as indicated. Panel 2 compares ERp18 KO cells to ERp18 KO cells that have been transfected with ERp18 (RV).   Normalised BiP levels from (D ) were quantified. Error bars represent ± standard deviation for three independent experiments.   "
NCBI gene IDs:
"ERp18: 51060"
Figure legend: " Levels of ERp72 protein in wild type or ERp18 KO cells before and after ER stress induction with thapsigargin (TG). Panel 1 compares wild type and ERp18 KO cells as indicated. Panel 2 compares ERp18 KO cells to ERp18 KO cells that have been transfected with ERp18 (RV).   Normalised ERp72 levels from (F) were quantified. Error bars represent ± standard deviation for three independent experiments.   "
NCBI gene IDs:
"ERp18: 51060"
Figure legend: " A, B Wild type and ERp18 KO cells stably expressing ATF6α were treated with 5 µM TG for the indicated times. Cells were then subject to differential centrifugation to obtain membrane (A) and nuclear fractions (B) which were separated by SDS-PAGE under reducing conditions and analysed by western blotting for ATF6α. The positions of full-length ATF6α (ATF6-FL), cleaved membrane-associated ATF6α (ATF6-P) and nuclear translocated S2P cleaved ATF6α (ATF6-N) are as indicated. HDAC2 was used as a nuclear marker. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " (C) ATF6α was immunoisolated from wild type or ERp18 KO cells expressing ATF6α. Samples were separated by SDS-PAGE and ATF6α was detected by western blotting. HC indicates immunoglobulin heavy chains. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " (D, E) Blots of membrane and nuclear fractions respectively from ERp18 KO cells overexpressing ATF6α, either untreated (-) or treated (+) with 30 µM PF429242 (S1P inhibitor) prior to and following induction of ER stress with 10 mM DTT. The positions of ATF6-P, ATF6-M and ATF6-N are as indicated. The unprocessed ER-localised ATF6α (designated ER) and the O-linked glycan modified Golgi-translocated ATF6α (designated G) are indicated. HDAC2 was included as a nuclear marker. The blots in (D, E) confirm that ATF6-P is produced independently of S1P and also the absence of ATF6α processing to ATF6-N in the presence of the S1P inhibitor. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " HEK293T cells stably overexpressing wild type ATF6α or a D564G mutant were treated with 10 mM DTT as indicated to induce ER stress in the presence of the S1P inhibitor. Cell lysates were prepared, separated by SDS-PAGE and then probed with anti-ATF6α. Blot reveals the presence of O-linked glycan modified Golgi-translocated ATF6 (designated G), which is absent for the D564G mutant, confirming a lack of ER to Golgi trafficking. "
NCBI gene IDs:
"ATF6α: 22926"
Figure legend: " Wild type and ERp18 KO cell lines stably expressing ATF6α D564G were either left untreated (-) or treated (+) with 5 µg/ml brefeldin A for1 h prior to cell lysis. The anti-ATF6α western blot of membrane fractions reveals proteolytic processing by S1P to produce ATF6-M, only in the brefeldin A treated cells. Nuclear fractions confirm the presence of S2P cleavage product, ATF6-N following brefeldin A treatment. Anti-HDAC2 was used as a loading control for the nuclear fraction. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " A-C Wild type cells stably expressing ATF6α (A) or ERp18 KO cells stably expressing ATF6α (B), and ERp18 KO cells stably expressing ATF6α transiently transfected with ERp18 (RV cells) (C), were pre-treated with 30 µM PF429242 (S1P inhibitor) for 1 h prior to induction of ER stress with 10 mM DTT as indicated. "
NCBI gene IDs:
"ATF6α: 22926
ERp18: 51060"
Figure legend: " D-F Wild type cells transiently expressing ATF6 C467A (D), ATF6 C618A (E), or ATF6 DM (F) were treated as above. Whole cell lysates were separated by SDS-PAGE under reducing conditions and analysed by western blotting for ATF6α. The positions of unprocessed ER-localised ATF6α (ER) and the O-linked glycan modified Golgi-translocated ATF6α (Gol) are indicated as is ATF6-P. "
NCBI gene IDs:
"ATF6: 22926"
Figure legend: "(B) Western blot analysis of Atxn1 levels in cerebellar granule neurons (CGNs) after knockdown of Zbtb7a  and/or Zbtb7b  (n = 3). Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"Zbtb7a: 16969
Zbtb7b: 22724"
Figure legend: "(C)  Western blot  analysis of Atxn1 levels in the  cerebella of wild-type (n = 3), Zbtb7a+/- (n = 4), Zbtb7b+/- (n = 3), Zbtb7a+/-;  Zbtb7b+/- (n = 4)  mice. Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"Zbtb7a: 16969
Zbtb7b: 22724"
Figure legend: "(D)  Western blot analysis of Atxn1  levels in CGNs after overexpressing Zbtb7a and/or  Zbtb7b (n = 3). Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"Zbtb7a: 16969
Zbtb7b: 22724"
Figure legend: "(E) Genetic   interaction   of wild-type  human ATXN1(30Q) with either  ZBTB7A or ZBTB7B expressed in the Drosophila eyes. Co-expression of ATXN1(30Q) with ZBTB7A or ZBTB7B severely disrupted the external Drosophila eye structure and increased ATXN1  levels. Scale bar: 100 μm"
NCBI gene IDs:
"ATXN1: 6310
ZBTB7A: 51341
ZBTB7B: 51043"
Figure legend: "(C) Western blot analysis of ATXN1 levels after overexpression of ZBTB7B wild-type (WT), zinc-finger domain mutant (ZFmut) or BTB domain mutants (BTBmut1 and BTBmut2) (n = 3). Data information: Mean ± SD, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"ZBTB7B: 51043"
Figure legend: "(D)  qRT-PCR  results of Atxn1 mRNA levels in the cerebella of wild-type (n = 3), Zbtb7b+/- (n = 4), and Zbtb7b-/- mice (n = 4). Data information: Mean ± SD, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"Atxn1: 20238
Zbtb7b: 22724"
Figure legend: "(E) MA plot (M: log ratio; A: mean average) of the differentially expressed genes (DEG) analysis from RNA-seq  samples of the cells overexpressing either WT or ZFmut ZBTB7B. Four filters and the number of genes kept after applying each filter are described. Two genes that passed the 4th filter are labeled."
NCBI gene IDs:
"ZBTB7B: 51043"
Figure legend: "(G) qRT-PCR results of  RSK3 mRNA levels across different CRISPR  knockout cell lines (n = 2). Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"CRISPR: 
RSK3: 6196"
Figure legend: "(H) Epistatic relationship between ZBTB7B and  RSK3  in terms of ATXN1 regulation. ATXN1 levels were measured after overexpression of ZBTB7B in the RSK3 knockout cell line (sgRSK3) and control cell lines (WT and sgControl) (n = 3). ZBTB7B+RSK3: co-overexpression of both ZBTB7B and RSK3. Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"RSK3: 6196
ZBTB7B: 51043"
Figure legend: "(I) qRT-PCR results of RSK3 mRNA levels  after knockdown of ZBTB7B in Daoy  cells (n = 2). Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"RSK3: 6196
ZBTB7B: 51043"
Figure legend: "(J) qRT-PCR  results of  Rsk3 mRNA levels in the cerebella  of Zbtb7b conditional knockout mice (n = 6). Data information: Mean ± SD, *P&lt;0.05, ***P&lt;0.001, ****P&lt;0.0001, two-tailed t-test."
NCBI gene IDs:
"Rsk3: 20112
Zbtb7b: 22724"
Figure legend: "(K) Position frequency matrix of ZBTB7B binding DNA sequence and a table of the predicted ZBTB7B binding sites on human RSK3 promoter. Start and End position numbers are described based on TSS. The seventy base pairs upstream  RSK3 promotor includes top two potential binding sites and was used for luciferase assays. Data information: Mean ± SD, *P&lt;0.05, ***P&lt;0.001, ****P&lt;0.0001, two-tailed t-test."
NCBI gene IDs:
"RSK3: 6196
ZBTB7B: 51043"
Figure legend: "(L) Luciferase assay result of  RSK3  promoter in HEK 293T and HeLa cells transfected with either an empty or ZBTB7B expressing vector (n = 3). Data information: Mean ± SD, *P&lt;0.05, ***P&lt;0.001, ****P&lt;0.0001, two-tailed t-test."
NCBI gene IDs:
"RSK3: 6196
ZBTB7B: 51043"
Figure legend: "(A) Western blot analysis of ATXN1 after knockdown of RSK3 in Daoy  cells. Bottom left panel: qRT-PCR results of RSK3 mRNA. Bottom right panel: densitometry of the western blot image in the top panel (n = 3 except for shATXN1 where n = 1). Data information: Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"ATXN1: 6310
RSK3: 6196"
Figure legend: "(B) Western blot  analysis of ATXN1 after overexpression of RSK3, ZBTB7B, or eGFP in Daoy cells (n = 3). Data information: RSK3 antibody used in is only suitable for detecting overexpressed RSK3 but not for endogenous RSK3. Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"eGFP: 
RSK3: 6196
ZBTB7B: 51043"
Figure legend: "(C)  Western blot  analysis of  ATXN1(82Q) after overexpression of either wild-type or inactive RSK3 in Daoy  cells expressing  ATXN1(82Q) (n = 3). Data information: RSK3 antibody used in is only suitable for detecting overexpressed RSK3 but not for endogenous RSK3. Mean ± SD, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"ATXN1: 6310
RSK3: 6196"
Figure legend: "(A)  Images of  Drosophila eyes expressing  human ATXN1(82Q) with either knockdown or overexpression of the Drosophila RSK3 homolog Sk6II. Scale bar: 100 μm (top) and 20 μm (bottom)."
NCBI gene IDs:
"ATXN1: 6310
RSK3: 33139
Sk6II: 33139"
Figure legend: "(B) Improvement of motor performance by the knockdown of Sk6II  in  Drosophila SCA1 model expressing ATXN1(82Q) in the central nervous system. Mean ± SEM, *P &lt; 0.05, Linear mixed-effect model ANOVA, n = 10/genotype, four times of measurements."
NCBI gene IDs:
"ATXN1: 6310
Sk6II: 33139"
Figure legend: "(C) A representative  image of a  mouse brain  4 weeks after AAV injection of shRsk3 into 12 weeks old SCA1 mice (Atxn1154Q/2Q) cerebellum. Scale bar: 2 mm."
NCBI gene IDs:
"Atxn1: 20238
Rsk3: 20112"
Figure legend: "(D)  Western blot analysis of wild-type (2Q) and mutant Atxn1 (154Q) in the cerebella of 16 weeks old SCA1 mice  dissected 4 weeks after the stereotaxic injection of AAV9 harboring shControl (n = 3), shRsk3 (n = 3), or shAtxn1 (n = 2). Mean ± SD, *P&lt;0.05, ***P&lt;0.001 ****P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"Atxn1: 20238
Rsk3: 20112"
Figure legend: "(E) Western blot  analysis of wild-type (2Q) and mutant  Atxn1 (154Q) in the  brainstem  (BS) and  cerebella (CBM) of 8 weeks old Atxn1154Q/2Q (n = 6) and Atxn1154Q/2Q;  Rsk3+/- mice (n = 7). Mean ± SD, *P&lt;0.05, **P&lt;0.01, two-tailed t-test."
NCBI gene IDs:
"Atxn1: 20238
Rsk3: 20112"
Figure legend: "(F) Rotarod motor performance test result of Atxn1154Q/2Q; Rsk3+/- mice at 11-12 weeks old. Mean ± SEM, *P&lt;0.05, two-way ANOVA, n = 35, 31, 34, 38 for WT, Rsk3+/-, Atxn1154Q/2Q, Atxn1154Q/2Q; Rsk3+/- genotype, respectively."
NCBI gene IDs:
"Atxn1: 20238
Rsk3: 20112"
Figure legend: "(B) Representative BaseScope assay images of Rsk3 in the whole brain sagittal sections of wild-type (top) and Rsk3-/- mice (bottom) at 5-6 weeks old. The areas within the black squares are magnified in the right panels in each genotype. Scale bar = 2 mm in whole  brain images; 50 μm in the magnified images. Data information:  BS: brainstem, CBM: cerebellum"
NCBI gene IDs:
"Rsk3: 20112"
Figure legend: "(C) Representative  IF images  of Msk1 in the whole brain sagittal  sections of wild-type (top) and Msk1-/- mice  (bottom) at 6 months old. The areas within the white squares are magnified in the right panels in each genotype. Scale bar = 2 mm in whole brain images; 50 μm in the magnified images. Data information: BS: brainstem, CBM: cerebellum"
NCBI gene IDs:
"Msk1: 73086"
Figure legend: "(G) Kaplan-Meier survival  graphs of SCA1 mice with heterozygous knockout of either one or both kinases. The data of Msk1+/-, Rsk3+/-, and Msk1+/-; Rsk3+/- mice overlap those of wild-type mice. *P&lt;0.05, Gehan-Breslow-Wilcoxon test, n = 15, 15, 12, 13, 22, 11, 20, 19 following the order of genotypes in the legend on top of the figure, respectively."
NCBI gene IDs:
"Rsk3: 20112
Msk1: 73086"
Figure legend: "(C) Purified GST‐tagged NixΔTM was cleaved from the GST moiety by thrombin, purified and used for precipitation by GST fusion proteins. Precipitated proteins were analysed by western blotting (WB) with Nix antibodies. Ponceau S staining was used to visualize GST‐fusion proteins."
NCBI gene IDs:
"Nix: 665"
Figure legend: "(C) Lysates of COS7 cells transfected with Flag‐wt‐Nix or the indicated mutants were incubated with immobilized GST‐LC3A. The coprecipitated proteins were detected by western blotting (WB) with Flag antibodies. BNIP3, Bcl2/E1B 19kDa‐interacting protein 3‐like protein; Dr, Danio reio; GST, glutathione‐S‐transferase; Hs, Homo sapiens; LIR, LC3‐interacting region; Mm, Mus musculus; wt, wild type; Xl, Xenopus laevis."
NCBI gene IDs:
"Nix: 665"
Figure legend: "Nix recruits EGFP‐GABARAP‐L1 to stressed mitochondria in reconstituted Nix−/− MEFs. Nix−/− MEFs cotransfected with EGFP‐GABARAP‐L1 and wt‐Nix (A), or Nix‐W35A (B), were incubated with and without mitochondrial poison CCCP for 3 h. Cells were then fixed and analysed for colocalization between EGFP‐GABARAP‐L1 and Nix (stained with Flag antibodies). The final panel in each row shows colocalization between Flag‐Nix‐Cy3 and GABARAP‐L1 using the 'colocalization' highlighter plug‐in for ImageJ software (NIH, Bethesda, MA, USA)."
NCBI gene IDs:
"Nix: 12177
GABARAP‐L1: 56486"
Figure legend: "(A) Flow cytometry of reticulocyte‐enriched blood, cultured in vitro for 3 days, stained with MTR. The blood was taken from mice transplanted with Nix−/− bone marrow and reconstituted with wt‐Nix and Nix‐W35A viruses. Reticulocytes were induced by phenylhydrazine treatment. The viruses also express GFP."
NCBI gene IDs:
"Nix: 12177"