EMBO/SourceData huggingface dataset:
Figure legend: "F Volcano plot  DNA methylation  represents all DMRs in mRNA  (left) and enhancer  (right) regions. Cutoffs (vertical and horizontal dashed lines) used to identify DMRs  between M-SCT  floxed and M-SCT  KO mice. The vertical lines correspond to twofold up and down, respectively, and the horizontal line represents a P value of 0.01. Data information: Statistical significance was determined using Two-tailed Student's t test."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "I, J Schematic diagram showing the CpG islands of Mus musculus PRDM16  (I) and CIDEA  (J) genes. The conditions to be met by CpG islands identified were: 200-300 bp length, CG content of &gt; 50% and observed CpG content vs expected CpG content &gt; 0.6. Methylation  levels of each CpG islands in PRDM16  (I) and CIDEA  (J) are presented as the fold change relative to the average value of the M-SCT  floxed group and β-actin promoter was used as a negative control (n = 3 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not statistically significant."
NCBI gene IDs:
"β-actin: 11461
CIDEA: 12683
PRDM16: 70673
SCT: 319229"
Figure legend: "A Western blots  analysis of DNMT1, DNMT3a, DNMT3b  and HSP90  in Ing  from M-SCT  KO and M-SCT floxed mice  with HFD."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "B Relative mRNA levels of DNMT1, DNMT3a  and DNMT3b  in Ing  from M-SCT  KO and M-SCT  floxed mice  with HFD  (n = 8). Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. ns, not statistically significant."
NCBI gene IDs:
"DNMT1: 13433
DNMT3a: 13435
DNMT3b: 13436
SCT: 319229"
Figure legend: "C Western blots analysis of p-AMPKα, AMPKα, p-ACC, ACC, DNMT1  and HSP90  in embryo  of M-SCT  KO and M-SCT  floxed mice  at E18.5."
NCBI gene IDs:
"SCT: 319229"
Figure legend: "G Western blot  analysis of p-AMPKα, AMPKα, p-ACC, ACC, DNMT1  and HSP90  in AMPK+/+ or AMPKα1/α2  double knockout (AMPK-/-) MEF  cells treated with 10 nM SCT  for 24 h."
NCBI gene IDs:
"AMPK: 105787
AMPKα1: 105787
α2: 108079"
Figure legend: "H, I Western blot analysis of Flag-DNMT1  in Flag-DNMT1-transfected HEK293T  cells treated with AICAR  with indicated dose or time  periods."
NCBI gene IDs:
"Flag: 
DNMT1: 1786"
Figure legend: "L AMPK+/+ or AMPK-/- MEF  cells were transfected with indicated plasmids and then lysed, followed by IP  and IB  analysis."
NCBI gene IDs:
"AMPK: 105787"
Figure legend: "I Relative mRNA levels of UCP1, PGC1α, PRDM16, CIDEA  and DIO2  in iWAT  from M-HFD  and M-HFD+SCT mice  with HFD  (n = 5 per group). Data information: Statistical significance was determined using Two-tailed Student's t test. Data are presented as mean ± SD. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"CIDEA: 12683
DIO2: 13371
PGC1α: 19017
PRDM16: 70673
UCP1: 22227"
Figure legend: "(D, E) representative confocal micrographs and quantification of LC3-II accumulation in CTNSWT and CTNS-/- cells in presence and absence of 25 nM bafilomycin (BafA1) for 4 hrs, respectively (n = 3). Scale bars are 20 µm."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(F, G, H) Western blotting and densitometric analyses for LC3-II/LC3-I ratio and SQSTM1 protein levels in CTNSWT, CTNS-/-, and CTNSPatient cells cultured in the presence or in the absence of 25 nM BafA1 for 4 h, respectively (n = 3)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(I, J) Representative confocal micrographs and quantification of DQ-BSA and BSA in CTNSWT, CTNS-/-, and CTNSPatient cells, respectively (n = 7-14 quantified images). Scale bars are 20 µm."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(D) List of metabolites that were shared and significantly altered in both the CTNS-/- and CTNSPatient cells compared to control cells (n = 6)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(I) List of proteins that were significantly upregulated and downregulated in CTNS-/- cells compared to control cells (n = 3). AKGDH; Alpha-ketoglutarate dehydrogenase, LIPA; Lysosomal acid lipase, ACP2; Lysosomal acid phosphatase, CTSS; Cathepsin S, CTSC; Dipeptidyl peptidase, IGF2R; Cation-independent mannose-6-phosphate receptor, SORT1; Sortilin, CAT; Catalase, CTSA; Lysosomal protective protein, and SOD; Superoxide dismutase."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(B, C, D) ROS production (ROS/mg protein) in CTNSWT, CTNS‑/-, and CTNSPatient cells upon starvation for 4 hrs in the presence and absence of DMKG (4 hrs), respectively (n = 3)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "Representative confocal micrographs (scale bars are 20 µm) of caspase 3/7 activation in DMKG (2 mM)-treated CTNSWT, CTNS‑/-, and CTNSPatient cells for 24 hrs (n = 3)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(H, I) Western blotting and densitometric analyses for LC3-II/LC3-I ratio in CTNSWT, CTNS‑/-, and CTNSPatient cells cultured in the presence or in the absence of BafA1 (25 nM) and DMKG (2 mM) for 4 hrs, respectively (n = 3)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(E), Proteomic analysis of CTNS-/- cells treated with cysteamine, bicalutamide, and a combination of cysteamine and bicalutamide, respectively (n = 3). AKGDH; Alpha-ketoglutarate dehydrogenase, GLUD1; GLUD2; Mitochondrial glutamate dehydrogenase 1/2, IGF2R; Cation-independent mannose-6-phosphate receptor, GSTK1; Glutathione S-transferase kappa 1, COX6B1; Cytochrome c oxidase subunit 6B1, ACACA; Acetyl-CoA carboxylase 1-Biotin carboxylase, GLS; Glutaminase kidney isoform, mitochondrial, CASP3; Caspase-3, NPC1; Niemann-Pick C1 protein."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(D, E) Representative confocal micrographs and quantification of DQ-BSA in CTNSWT, and CTNS-/- cells upon treatment with bicalutamide. Scale bars are 20 µm (n = 8-14 quantified images)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(F) Quantification of cystine levels (nmol/mg protein) by HPLC-MS/MS in CTNS-/- in the absence of the drug (NT) (n = 4) or upon treatment with cysteamine (n = 6), bicalutamide (n = 6), and a combination of cysteamine and bicalutamide (n = 6). (G) Quantification of cystine levels (nmol/mg protein) by HPLC-MS/MS in CTNSPatient in the absence of the drug (NT) (n = 4) or upon treatment with cysteamine (n = 4), bicalutamide (n = 4), and a combination of cysteamine and bicalutamide (n = 6)."
NCBI gene IDs:
"CTNS: 1497"
Figure legend: "(A) Immunocytochemistry of patient-derived cystinotic tubuloids (CNTSPatient-1 and CTNSPatient-2) and healthy kidney tissue-derived control tubuloids (CNTSWT-1 and CTNSWT-2) for PAX8, TP63 and F-actin. Scale bar = 100 µm."
NCBI gene IDs:
"CNTS: 1497
CTNS: 1497"
Figure legend: "(C, D) Quantification of cystine levels (nmol/mg protein) by HPLC-MS/MS in two different patient-derived cystinotic tubuloids in the absence of the drugs (NT) or upon treatment with cysteamine (100 μM), bicalutamide (35 μM) or cysteamine (100 μM)-bicalutamide (35 μM) combination treatment (n = 3). (E) αKG levels measured in patient-derived cystinotic tubuloids (CNTSPatient-1 and CTNSPatient-2) in the absence of the drugs (NT) or upon treatment with cysteamine, bicalutamide or cysteamine-bicalutamide combination treatment using metabolomics (n = 3). "
NCBI gene IDs:
"CNTS: 1497
CTNS: 1497"
Figure legend: "(I) Survival rates in ctns-/- zebrafish upon treatment with cysteamine, bicalutamide, and a combination of cysteamine and bicalutamide (n=40 embryos per group)."
NCBI gene IDs:
"ctns: 553594"
Figure legend: "(K) Hatching rates in surviving ctns-/- zebrafish evaluated at 72 and 96 hrs post fertilization (hpf) with cysteamine, bicalutamide, and a combination of cysteamine and bicalutamide (n=40 embryos per group)."
NCBI gene IDs:
"ctns: 553594"
Figure legend: "(A-B) THP1 over-expressing IFITM3, IFITM3-Y20F, IFITM3-∆21 or control were pre-treated or not with Amphotericin B for 1 hour and then transduced with a VSV-gp (A) or Measles-gp (B) pseudotyped LV. Transduction efficiencies were evaluated at FACS five days later. Fold rescue of transduction over the DMSO control was calculated. Data are shown as mean ± SEM (n=5-8 biological replicates run in technical duplicate). P values are for one sample t test versus Oe-Luc DMSO =1, ** for p&lt;0.01, *** for p&lt;0.001."
NCBI gene IDs:
"gp: 
Luc: 
IFITM3: 10410
gp: 1489834"
Figure legend: "(E-F) THP1 over-expressing IFITM3, IFITM3 lysin-less mutant (4KR) (E), IFITM3 CIL-lysin mutant (3KR) (F) or control were transduced with VSV-gp pseudotyped LV. Transduction efficiencies were evaluated by flow cytometry five days post-TD. Data represent the mean ± SEM (n=9-6 biological replicates run in technical duplicate). p values are for one sample t test verso Oe-Luc DMSO= 1. * for p&lt;0.05 ** for p&lt;0.01. **** for p&lt;0.0001 and ns for not significant."
NCBI gene IDs:
"Luc: 
IFITM3: 10410
gp: 1489834"
Figure legend: "(G-H) THP1 over-expressing IFITM1, IFITM1-3KR (G), IFITM3, IFITM3-Y20F, IFITM3-Y20F-3KR (H) or control were transduced with Measles-gp pseudotyped LV. Transduction efficiencies were evaluated by flow cytometry five days post-TD. Data represent the mean ± SEM (n=4-9 biological replicates run in technical duplicate). p values are for one sample t test verso Oe-Luc DMSO= 1. * for p&lt;0.05 ** for p&lt;0.01.**** for p&lt;0.0001."
NCBI gene IDs:
"gp: 
Luc: 
IFITM1: 8519
IFITM3: 10410"
Figure legend: "(A-B) Immunofluorescence images were performed using TCS SP5 Leica confocal microscope, 60x with oil on THP1 over-expressing IFITM3-WT, IFITM3-4KR, IFITM3-3KR or IFITM3-Y20F (A) (n=22 images acquired from 3 biological replicates) and primary monocytes derived macrophages (MDM) over-expressing IFITM3-WT, IFITM3-4KR, IFITM3-3KR (B) (n=12 images acquired from 3 independent donors). Co-localization (purple areas marked by white arrows) of IFITM3 (in red) with the lysosome associated membrane protein 1 (LAMP1) marker (in blue) was evaluated. Representative zoomed images are shown,"
NCBI gene IDs:
"IFITM3: 10410"
Figure legend: "(D) IFITM3-IFITM3 interactions (red dots) were evaluated by proximity ligation assay (PLA) in THP1 knock out for endogenous IFITM3 and expressing either HA-IFITM3 and His-IFITM3, HA-3KR and His-3KR or His-dimer mutant IFITM3 (D.M) and HA-dimer mutant. Images were acquired on TCS SP5 Leica confocal microscope 60x with oil. Representative images are shown (n=8 images of 4 biological replicates), scale bar 40μm."
NCBI gene IDs:
"HA: 
His: 
IFITM3: 10410"
Figure legend: "(B) K562 were prestimulated with IFNα and then transduced with VSV-gp pseudotyped LV. Transduction was evaluated five days after TD (mean ± SEM, n=4 biological replicates run in technical duplicate). Data are represented as Folds over the Oe-Luc control. p values are for one sample t test versus -IFNα = 1. ns for not significant."
NCBI gene IDs:
"Luc: 
gp: 1489834"
Figure legend: "(F) K562 over-expressing IFITM1, IFITM1-3KR or control were transduced with Measles-gp pseudotyped LV. Transduction efficiency was evaluated five days post-TD (mean ± SEM, n=4 biological replicates run in technical duplicate). p values are for one sample t test versus Oe-Luc = 1. **** for p&lt;0.0001."
NCBI gene IDs:
"gp: 
Luc: 
IFITM1: 8519"
Figure legend: "K562 over-expressing IFITM3, IFITM3-Y20F, IFITM3-Y20F-3KR or control were pre-exposed or not to Ampho B and then transduced with Measles-gp (G), BaEV-gp (H) pseudotyped LV. Transduction efficiencies were measured by flow cytometry five days after TD (mean ± SEM, n=5-4-4 biological replicates run in technical duplicate). p values are for one sample t test versus Oe-Luc DMSO=1 or unpaired t test for Ampho B versus DMSO. * for p&lt;0.05, ** for p&lt;0.01, *** for p&lt;0.001, **** for p&lt;0.0001."
NCBI gene IDs:
"gp: 
Luc: 
gp: 22318530
IFITM3: 10410"
Figure legend: "(A) Interaction between IFITM3 wild-type or 3KR and Phosphatydinositol-3 phosphate (PIP3) (red dots) was evaluated by proximity ligation assay. Images were acquired using TCS SP5 Leica confocal microscope 60x with oil in THP1 knock out for endogenous IFITM3 and expressing either IFITM3 wild-type or IFITM3-3KR. Number of foci were counted on imageJ and normalized over the number of nuclei (mean ± SEM, n=5 biological replicates). White arrows indicate foci, scale bar 40μm."
NCBI gene IDs:
"IFITM3: 10410"
Figure legend: "(B) Phosphatydinositol-3 phosphate (PIP3) levels were measured by immunofluorescence in THP1 over-expressing the His-IFITM3/HA-IFITM3 and His-3KR/HA-3KR. PIP3 was quantified via Imagej (mean ± SEM, n=5 biological replicates)."
NCBI gene IDs:
"HA: 
His: 
IFITM3: 10410"
Figure legend: "(C) THP1 over-expressing IFITM3 or control were transduced with VSV-gp pseudotyped LV in presence or absence of Rapamycin (Rapa). Transduction efficiency was measured at FACS five days post-TD (mean ± SEM, n=6 biological replicates run in technical duplicate. p values are for one sample t test versus Oe-Luc DMSO=1 and Mann Whitney test for Rapa vs DMSO. ** for p&lt;0.01, **** for p&lt;0.0001."
NCBI gene IDs:
"Luc: 
IFITM3: 10410
gp: 1489834"
Figure legend: "(D) THP1 over-expressing IFITM3-Y20F-3KR or control were exposed or not to Rapamycin and transduced with Measles-gp pseudotyped LV. Transduction efficiency was evaluated by flow cytometry five days post-TD (mean ± SEM, n=4 biological replicates run in technical duplicate). p values are for one sample t test versus Oe-Luc DMSO=1. ** for p&lt;0.0."
NCBI gene IDs:
"gp: 
Luc: 
IFITM3: 10410"
Figure legend: "(E) Hematopoietic stem and progenitor cells (HSPC) were transduced with VSV-gp or Measles-gp pseudotyped LV in presence or absence of Rapamycin. Transduction was evaluated at FACS five days later measuring the % of BFP expression (mean ± SEM, n=4 biological replicates run in technical duplicate). p values are for Mann Whitney test. * for p&lt;0.05."
NCBI gene IDs:
"gp: 
gp: 1489834"
Figure legend: "(E) PIP3 levels were measured by immunofluorescence (scale bar 40μm) and quantified as integrated density with ImageJ software in THP1 and K562 over-expressing IFITM3 (mean ± SEM, n=3 biological replicates). p values are for Mann Whitney test, * for p&lt;0.05."
NCBI gene IDs:
"IFITM3: 10410"
Figure legend: "(A) Calu3 over-expressing IFITM1, IFITM2, IFITM3, PM-mutant IFITM3 Y20A or control were transduced with SARS-CoV2 Spike-pseudotyped LV (mean ± SEM, n&gt;6 biological replicates run in technical duplicate). Transduction efficiency was measured by FACS five days after transduction, p values are for one sample paired T test versus Oe-Luc Ctrl=1, **for p&lt;0.01."
NCBI gene IDs:
"Luc: 
IFITM1: 8519
IFITM2: 10581
IFITM3: 10410
Spike: 43740568"
Figure legend: "(B-C) Calu3 cells over-expressing IFITM1, IFITM2, IFITM3, IFITM3 Y20A or control were infected with SARS-CoV2. Viral titer was calculated by plaque assay in Vero E6 cells (mean ± SEM, n=4 biological replicates run in technical duplicate). p values are for Mann Whitney test, * for p&lt;0.05, *** for p&lt;0.001."
NCBI gene IDs:
"IFITM1: 8519
IFITM2: 10581
IFITM3: 10410"
Figure legend: "(G) Calu3 over-expressing IFITM1, IFITM3 or control were challenged to SARS-CoV2 Omicron pseudotyped LV. Transduction efficiency was evaluated 5 days post-transduction (mean ± SEM, n=7 biological replicates run in technical duplicate). p values are for one sample T test versus Oe-Luc Ctrl=1, **for p&lt;0.01, ****for p&lt;0.0001."
NCBI gene IDs:
"Luc: 
IFITM1: 8519
IFITM3: 10410"
Figure legend: "(H-I) Calu3 cells over-expressing IFITM1, IFITM2, IFITM3 or control (H) or IFITM3, Y20A or control (I) were infected with Omicron at MOI 1. Viral supernatants were collected at different time points and tittered on Vero E6 cells. Data are presented as mean ± SEM (n=4-4 biological replicates run in technical triplicate). p values are for Mann Whitney test , **for p&lt;0.01, ***for p&lt;0.001, ****for p&lt;0.0001."
NCBI gene IDs:
"IFITM1: 8519
IFITM2: 10581
IFITM3: 10410"
Figure legend: "(A-B) VSV (A) or ZIKV (B) viral supernatants were collected one day post-infection in THP1 (A) or Calu3 (B) over-expressing IFITM3, IFITM3 lysin-less mutant (4KR), IFITM3 CIL-lysin mutant (3KR) or control and titered on VERO E6 cells. Data represent the mean ± SEM (n=5-3 biological replicates run in technical duplicate). p values are for Mann Whitney test. * for p&lt;0.05, ** for p&lt;0.01."
NCBI gene IDs:
"IFITM3: 10410"
Figure legend: "(I) IFITM3 and PIP3 level were measured by immunofluorescence (scale bar 100µm) and quantified as integrated density with ImageJ software in Calu3 infected or not with Omicron (n=3 biological replicates). p values are for Mann Whitney test, ** for p&lt;0.01. (J) PIP3 coupled to a specific PIP carrier (PIP3+Carr Ctrl) or Carr Ctrl were provided to Calu3 over-expressing IFITM3, control or KO for IFITM3 prior to infection with SARS-CoV2 Omicron. Infectious supernatants were collected after three days and tittered in Vero E6 cells (mean ± SEM, n=4 biological replicates run in technical duplicate ). p values are for Wilcoxon signed ranked test, * for p&lt;0.05. The inhibitory effect of exogenous PIP3 on infection is represented as Fold versus Carr Ctrl (mean ± SEM, n=4 biological replicates run in technical duplicate ). p values are for Mann Whitney test, * for p&lt;0.05."
NCBI gene IDs:
"IFITM3: 10410"
Figure legend: "B HXT2 mRNA localization correlate with that of the protein. FISH with an HXT2-specific probe. Early in the cell cycle the mRNA is predominantly found in the mother cell (white arrowheads) while it is equally distributed after meta-to-anaphase transition (MAT) (yellow arrowheads). C Quantification of (B). The fluorescence intensities from mother and bud were independently measured and the ratio of the signal from the mother over the signal from the bud was calculated. Values &gt; 1 indicate a stronger signal in the mother, &lt; 1 a stronger signal in the bud and around 1 equal distribution. Three independent experiments with 50 mother cells and 50 buds per experiment were analyzed. Boxes represent the interquartile range from the 25th to the 75th percentile with the median. Whiskers represent the 10th and 90th percentile, respectively. "
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "D Changes of HXT2 mRNA localization appear to be independent of the cytoskeleton. Cells were either treated for 15 min with 30 mg/ml latrunculin A or with 30 mg/ml benomyl. White arrowheads point to small-budded and yellow arrowheads to large budded cells. E Quantification of (D) of three in dependent experiments with at least 50 cells each. Values &gt; 1 indicate a stronger signal in the mother, &lt; 1 a stronger signal in the bud and around 1 equal distribution. Boxes represent the interquartile range from the 25th to the 75th percentile with the median. Whiskers represent the 10th and 90th percentile, respectively. "
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "A, Inhibiting translation by either applying Cycloheximide (CHX) or Verrucarin A (VerruA) leads to the retention of HXT2 mRNA in the mother cell after MAT. Chloro: chloroform; solvent control for VerruA; NT: not treated; control for CHX."
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "C CHX treatment leads to increased transcript stability. Quantitative PCR of HXT2 mRNA in CHX-treated cells in comparison to prt1-1 mutant cells. The data represents 3 independent experiments and the standard deviation is given."
NCBI gene IDs:
"HXT2: 855023
prt1-1: 854543"
Figure legend: "D Translation of HXT2 mRNA is more active late in the cell cycle. The HXT2 mRNA level bound to either di-/trisomes and polysomes (≥ 4 ribosomes) over monosomes was determined by extraction of mRNA from cells elutriated and harvested at G1/S or M-phase followed by qPCR. At G1/S similar levels of the HXT2 mRNA were found in the different ribosome fractions, whereas in M-phase more mRNA was bound to polysomes, suggesting a more active translation in M-phase."
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "E The localization of HXT2 mRNA is independent of the Signal Recognition Particle (SRP). Deletion of SRP54 does not affect the retention in the mother cell or the equal distribution of HXT2 mRNA."
NCBI gene IDs:
"HXT2: 855023
SRP54: 856203"
Figure legend: "A Deletion of SCP160, ASC1, or BFR1 increases HXT2 mRNA signal in the bud. FISH experiment with deletions in various RNA binding proteins."
NCBI gene IDs:
"ASC1: 855143
BFR1: 854373
HXT2: 855023
SCP160: 853365"
Figure legend: "B The effect of ∆scp160 on HXT2 mRNA localization is independent of its increased ploidy. Scp160 truncations that lack either the last two (∆C2) or the last four (∆C4) KH domains or a Tet-off SCP160 construct, in which expression is blocked by the addition of doxycycline (Doxy)"
NCBI gene IDs:
"HXT2: 855023
SCP160: 853365
scp160: 853365
Scp160: 853365"
Figure legend: "D Hyperactivation of PKA drives accumulation of HXT2 mRNA in the bud. Treatment of cells with forskolin (+Forsko) or using a ∆bcy1 strain, causes HXT2 mRNA to be bud-enriched."
NCBI gene IDs:
"bcy1: 854778
HXT2: 855023"
Figure legend: "E HXT2 mRNA enrichment upon refeeding is Ras2-dependent."
NCBI gene IDs:
"HXT2: 855023
Ras2: 855625"
Figure legend: "A Table of the glucose affinity of the four most important hexose transporters. B FISH of HXT1, 3 and 4 under glucose rich conditions. White arrowheads depict cells before, yellow arrowheads cells after MAT. C Quantification of (B). "
NCBI gene IDs:
"HXT1: 856494"
Figure legend: "E HXT2 mRNA is the only HXT mRNA showing enrichment in the bud after MAT upon transient PKA activation."
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "F Elutriation confirms enrichment of HXT2 mRNA in daughter cells upon refeeding. Daughters and mothers were separated through elutriation. qPCR was performed to determine the relative levels of different HXTs and ASH1 mRNA in both fractions."
NCBI gene IDs:
"ASH1: 853650
HXT2: 855023"
Figure legend: "G Enrichment of HXT2 mRNA in the bud leads to higher Hxt2-GFP transporter levels at the plasma membrane of the daughter cells. Daughter cells were enriched through elutriation. Quantification of the fluorescence intensity of the plasma membrane from life cell imaging from three independent experiments in which at least 50 cells were analyzed per condition and experiment. Western blot analysis confirms the higher amount of Hxt2-GFP transporter protein present in daughter cells under glucose-shift conditions"
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "A RNA degradation is not essential for asymmetric HXT2 mRNA localization. FISH of wild-type and ∆xrn1 cells."
NCBI gene IDs:
"HXT2: 855023
xrn1: 852702"
Figure legend: "B The cytoskeleton contributes to HXT2 mRNA enrichment in the bud. Cells were treated upon glucose addition with either 120 mg/ml benomyl for 15 min, 30 mg/ml latrunculin A for 30 min or 200 µM CK-666 for 30 min. HXT2 mRNA was detect by FISH."
NCBI gene IDs:
"HXT2: 855023"
Figure legend: "C Asymmetric loading of HXT2 mRNA onto the nuclear envelope and enrichment in the bud requires Kar9 but not Bim1 function. HXT2 mRNA FISH with ∆bim1 and ∆kar9 strains. Arrows point to a nucleus with HXT2 signal. D Quantification of the daughter cell enrichment from data displayed in (C). E Quantification of the HXT2 mRNA signal in cells with two nuclei in the mother cell from the experiment depicted in (C). Percentage of cells with asymmetric HXT2 mRNA staining is displayed. "
NCBI gene IDs:
"bim1: 856736
HXT2: 855023
kar9: 855859"
Figure legend: "A Deletion of NUP2 and MLP1/2 reduced HXT2 mRNA enrichment in the bud. FISH under glucose shift conditions with strains in which different NPC components were deleted. B Quantification of (A). "
NCBI gene IDs:
"HXT2: 855023
MLP1: 853970
NUP2: 851048"
Figure legend: "C HXT2 mRNA is on the nuclear envelope after MAT upon glucose shift. FISH-IF experiment with a probe against HXT2 mRNA and an antibody against the HA tag of the nuclear pore complex component Nup84-HA. White arrows point to HXT2 mRNA signals on the nuclear envelope."
NCBI gene IDs:
"HXT2: 855023"