EMBO/SourceData huggingface dataset:
Figure legend: "G. Control or OMA1 KO HCT116 cells were cultured in hypoxia (1% O2) for 0, 24, or 48 hours. Cell lysates were assessed by Western blotting with anti-NDUFB5, anti-NDUFB6, anti-COX4L1, anti-NDUFA4 antibodies, or anti-β-Actin. The relative protein levels were further evaluated by densitometry analysis using ImageJ software and were presented as bar graphs in the right panels. Error bars are presented as mean ± SD by a two-way ANOVA (n = 3 independent experiments). *p&lt;0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "A. Mitochondria isolated from tumors (T) or adjacent normal (N) tissues of WT and OMA1-/- mice were subjected to blue native PAGE (BN-PAGE), and respiratory chain complexes were analyzed by Western blotting with anti-NDUFB8 (Complex I), anti-SDHA (Complex II), anti-COX4L1 (Complex IV), and anti-ATP5A1 (Complex V, ATP synthase) antibodies. SDS-PAGE with antibody against HSPD1 was used as a loading control. Relative protein levels were further evaluated by densitometry analysis using ImageJ software. Error bars are presented as mean ± SD by a two-way ANOVA (n = 3 independent experiments). *p&lt;0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "B. Digitonin-solubilized mitochondria were isolated from control and OMA1 KO HCT116 cells cultured in normoxia or hypoxia (1% O2) for 12 hours, and then analyzed by BN-PAGE and immunoblotting using the indicated antibodies against NDUFB8 (Complex I), SDHA (Complex II), NDUFA4 (Complex IV), and ATP5A1 (Complex V). SDS-PAGE with antibody against HSPD1 was used as a loading control. Relative protein levels were further evaluated by densitometry analysis using ImageJ software. Error bars are presented as mean ± SD by a two-way ANOVA (n = 3 independent experiments). N.S., not significant, *p&lt;0.05."
NCBI gene IDs:
"OMA1: 115209"
Figure legend: "C. The lysates of adjacent normal tissues (N) or tumors (T) from WT and OMA1-/- mice were analyzed by Western blotting with antibodies against NDUFA4, COX4L1, NDUFB5, DNUFB6, UQCRC2, or Tubulin. Relative protein levels were further evaluated using ImageJ software. Error bars indicate the mean ± SD by a two-way ANOVA (n = 3 independent experiments). *p&lt;0.05, ***p &lt; 0.001."
NCBI gene IDs:
"OMA1: 67013"
Figure legend: "A. Immunoblot  analysis of ACE2 expression  in the lysate of human lung A549  cells overexpressing ACE2  compared to non-transduced (-) A549  cells. Calnexin served as loading control."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "B, C. Illustration of the Incucyte readout. Infection of A549  or ACE2-expressing A549  cells with  SARS-CoV-2 encoding a GFP  expression cassette. Infection  of cells was monitored microscopically  as green fluorescence through  live-cell imaging at 24 hours post infection  (hpi) (B) or during the  time-course  of 48 h and shown as the mean of the GFP-positive area relative to the whole area covered by cells in the same well (C). Error bars represent means ± SD derived from biological triplicates (N = 3). Scale bar, 500 µm."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "D. A549-ACE2  cells were treated for 6 hours with different concentrations of indicated inhibitors and then infected with SARS-CoV-2-GFP. GFP-fluorescence relative to DMSO-treated control cells was determined 48 hpi as described in (C). Two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed for every indicated concentration compared to DMSO  (0 µM). Asterisks indicate significance compared to DMSO  (N = 3-6). Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "E. A549-ACE2  cells were treated for 30 min with PMA  at indicated concentrations and then infected with SARS-CoV-2-GFP. Infection  of cells was monitored as in C. Two-way ANOVA with Tukey's post-hoc multiple comparison test. Asterisks indicate significance compared to the DMSO  control of each time point  (N = 9). Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "F, A549-ACE2  cells were treated with apratastat, DPC-333 at indicated concentrations. Experiment was conducted and analyzed as in (D). Two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed for every indicated concentration compared to DMSO  (0 µM). Asterisks indicate significance compared to DMSO  (N = 3-6). Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "G. A549-ACE2  cells were treated with apratastat, DPC-333 or BB94  at indicated concentrations. Experiment was conducted and analyzed as in (D). Two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed for every indicated concentration compared to DMSO (0 µM). Asterisks indicate significance compared to DMSO  (N = 3-6). Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "H, I. A549-ACE2  cells were treated for 1 h with indicated inhibitors (10 µM) or DMSO  before inoculation with GFP-encoding spike  pseudoparticles (VSV  pseudotyped with  SARS-CoV-2 spike  protein (VSV-S)) (H) or as a control LCMV glycoprotein  (VSV-GP) (I). At 16 hpi, infection  was analyzed by counting GFP-positive cells. Data are normalized to the number of infected cells in virus-only control wells without DMSO  (N = 3-5). One-way ANOVA with Tukey's post-hoc multiple comparison test. Asterisks indicate significance compared to DMSO. Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
ACE2: 59272"
Figure legend: "A, RT-qPCR quantification of  viral RNA (SARS-CoV-2 N gene  relative to RPLP0) upon infection  of A549-ACE2  cells with indicated SARS-CoV-2 strains at 24 hpi. Cells were pretreated with BB94  (10 µM) or DMSO  for 6 h. Data are normalized to DMSO  (N = 3-7). A two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed. Data information: Data are represented as means ± 95% CI from at least three (A, biological replicates *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272
N gene: 43740575
RPLP0: 6175"
Figure legend: "B.  RT-qPCR  quantification of  viral RNA  (SARS-CoV-2 N gene relative to RPLP0) upon infection  of A549-ACE2  cells with indicated  SARS-CoV-2 strains at 24 hpi. Cells were pretreated with BB94  (10 µM), DPC-333 (10 µM) or DMSO  for 6 h. Data are normalized to DMSO (N = 3-7). A two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed. Data information: Data are represented as means ± 95% CI from at least three , B) biological replicates *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272
N gene: 43740575
RPLP0: 6175"
Figure legend: "C. Normal Human Bronchial Epithelial cells (NHBE) from ten donors were infected with SARS-CoV-2. NHBE   cells were pre-treated with camostat mesylate (10µM), BB94  (1 µM or 10 µM) or DMSO  for 6 hours. RNA was isolated at 24 hpi and levels of SARS-CoV-2 N relative to RPLP0  from total cellular RNA was measured by RT-qPCR. Data are normalized to the DMSO  control (N = 5-10). Two-sided independent Student's t-test with Benjamini-Hochberg FDR correction. Data information: Data are represented as means ± 95% CI from at least five/ten biological replicates *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"RPLP0: 6175"
Figure legend: "A. Western Blot (WB) analysis of A549-ACE2  knock-out (KO) lines. Two different KO lines were generated using distinct CRISPR  guide RNAs for both ADAM10  and ADAM17. A non-targeting control (NTC) guide RNA served as control. Shown are two replicates for each cell line. Mature (m) and immature (im) forms of ADAM10  and ADAM17  are indicated. ACE2  levels remained unaffected.  α-tubulin  served as loading control. *: remaining, low amount of immature ADAM10  and ADAM17. The double KO (dKO) was generated from guide 1 of each ADAM10  and ADAM17. B. Quantification of the abundance of mature ADAM10  and ADAM17  protein forms from (A). Data are normalized to loading control tubulin  and to NTC (N = 3-4). The band of mADAM17 runs very close to three unspecific bands (marked with arrowheads) - one closely above and two closely below mADAM17 (A). Thus, quantification of remaining mADAM17 in the ADAM10  KO includes also these three unspecific bands. Two-sided independent Student's t-test with Benjamini-Hochberg FDR correction. Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"CRISPR: 
ACE2: 59272
ADAM10: 102
ADAM17: 6868"
Figure legend: "C. RT-qPCR  quantification of viral RNA (SARS-CoV-2 N gene relative to RPLP0) upon infection  of A549-ACE2  NTC or KO cells with SARS-CoV-2. Cells were treated with BB94  (10 µM) or DMSO  for 6 h prior to infection  and RNA was isolated at 24 hpi. Two-way ANOVA with Tukey's post-hoc multiple comparison test. Data are normalized to NTC DMSO  (N = 3). Data information: Data are represented as means ± 95% CI from at least three independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272
N gene: 43740575
RPLP0: 6175"
Figure legend: "D-G. UMAP of 63,103 cells from BAL fluid  derived from infected individuals (D) Individual cell types are indicated in color. (E) Normalized counts of SARS-CoV-2 reads, ADAM10, ADAM17, and TMPRSS2 gene expression levels. (F) Dot plot of ADAM10, ADAM17, and TMPRSS2  expression, grouped by disease severity. Expression levels are color-coded, the percentage of cells expressing the aforementioned genes is size coded. (G) Cells that are infected and additionally express ADAM17  (ADAM17+, red), ADAM10  (ADAM10+, blue), both ADAMs (ADAM10+ ADAM17+, purple), or TMPRSS2  (TMPRSS2+, orange) are indicated."
NCBI gene IDs:
"ADAM10: 102
ADAM17: 6868
TMPRSS2: 7113"
Figure legend: "B. Representative images of syncytia  formation assay. Large syncytia  were observed for NTC and ADAM17  KO cells, but were strongly reduced when ADAM10  was knocked-out, either alone or together with ADAM17  or upon inhibition with BB94  (10 µM). For both ADAMs, the cell lines obtained with gRNA sequence 1 were used. Images show GFP  fluorescence and Hoechst staining. Scale bars, 50 µm. C. Quantification of images from fluorescence microscopy (B). Left plot: the area of the fused cells in green (GFP) was quantified and normalized to the Hoechst  signal (blue) of the entire image to account for differences in cell density. Right plot: The nuclei  within syncytia  were determined by calculating the ratio between the Hoechst  signal within syncytia  and the total Hoechst  signal in the entire image. The data are normalized to NTC DMSO  (N &gt;= 8). Two-way ANOVA with Tukey's correction for multiple comparisons. All data are represented as means ± 95% CI of at least 3 independent experiments. **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ADAM10: 102
ADAM17: 6868"
Figure legend: "C. Representative images and quantification of a proximity ligation assay  (PLA) between the SARS-CoV-2 Spike  and ADAM17  in A549-ACE2  cells. Cells were infected with SARS-CoV-2 and placed on ice (4°C). After 2 h, cells were shifted to 37°C for 15 min or left on ice. Images are presented as overlay between the Nuclei  (Hoechst, blue) and the PLA  (pseudo-color: red) channel. Scale bar, 20 µm. The number of PLA  foci per cell was determined for the acquired images (N = 28-30). A two-sided independent Student's t-test with Benjamini-Hochberg FDR correction was performed. Data information: Data are represented as means ± 95% CI of at least 3 independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "D. A549-ACE2  cells were treated for 48 h with BB94  (10 µM), DPC-333 (5 µM) or DMSO. PMA  (25 ng/ml) was added for 3 h before harvest to stimulate ACE2   shedding. Concentration of the soluble ACE2  fragment was determined in the conditioned medium by ELISA  and is shown relative to the concentration in the DMSO  control (N = 6). One-way ANOVA with Tukey's post-hoc multiple comparison test. Data information: Data are represented as means ± 95% CI of at least 3 independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"ACE2: 59272"
Figure legend: "E. GFP-encoding spike  pseudoparticles (VSV  pseudotyped with SARS-CoV-2 spike protein (VSV-S) were incubated with indicated amounts of recombinant, soluble ACE2 (sACE2) or  bovine serum albumin (BSA) as a control prior to infection  of A549-ACE2  cells. At 16 hpi, infection  was analyzed by counting GFP-positive cells. Data are normalized to the number of infected cells in virus-only control wells without sACE2  or BSA  (N = 3). Two-sided independent Student's t test with Benjamini-Hochberg FDR correction. Data information: Data are represented as means ± 95% CI of at least 3 independent experiments. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001."
NCBI gene IDs:
"GFP: 
ACE2: 59272"
Figure legend: "Immunoblot  analysis of protein lysates from ARPE-19 cells expressing TFEB-WT-FLAG  or TFEB-S401A-FLAG  treated with 200 μM NaAsO2  or 250 nM Torin-1  for 1 h. Two different rabbit polyclonal antibodies raised against a TFEB-S401 phospho-specific peptide were tested."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from  ARPE-19 cells expressing TFEB-WT-FLAG, TFEB-S3A/R4A-FLAG  or TFEB-R245-247A-FLAG  treated with 200 μM NaAsO2  for 1 h. Immunoblots  are representative of at least three independent experiments."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from HeLa  cells stably expressing TFEB-WT-FLAG  incubated with the indicated kinase inhibitors for 1 h prior to the addition of 200 μM NaAsO2  for 1 h. Quantification of immunoblot  data shown in (A). Data are presented as mean ± SD using one-way ANOVA (unpaired) followed by Tukey's multiple comparisons test, (ns) not significant, and (****)p&lt;0.0001 from three independent experiments. Data information: n = 3 biological replicates (each dot represents a biological replicate)."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from HeLa   cells stably expressing TFEB-WT-FLAG  incubated with either 200 μM NaAsO2  for 1 h, EBSS  for 4 h, 100 ng/ml EGF   for the indicated times  or 37 μM Anisomycin  for 1 h. Before the addition of EGF  or Anisomycin, cells were serum  starved for 8 h."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from HeLa  cells stably expressing TFEB-WT-FLAG  exposed to 30J/m2 of UV-C and allowed to recover in complete medium for the indicated times. Cells were incubated with p38 MAPK inhibitor (20 μM, SB203580) for 1 h before  UV-C irradiation  and allowed to recover for 30 min in the presence of the inhibitor."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from HeLa  cells stably expressing TFEB-WT-FLAG  depleted of either p38 MAPK (α+β) or JNK1  or JNK2  or JNK(1+2) and incubated with 200 μM NaAsO2  for 1 h. Immunoblots  are representative of at least three independent experiments."
NCBI gene IDs:
"FLAG: 
JNK1: 5599
JNK2: 5601
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from Raw 264.7  cells stably expressing TFEB-WT-FLAG  incubated with 1μg/ml LPS  for the indicated times."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from  Raw 264.7 cells stably expressing TFEB-WT-FLAG  incubated with either 20 μM SB203580  or 250 nM Torin-1 for 1 h prior to the addition of 1 μg/ml LPS  for 30 min."
NCBI gene IDs:
"FLAG: 
TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates from naïve THP1-WT or TFEB-S401A knock-in (clone M17) cells treated with 50 ng/ml PMA  for the indicated times, PMA-differentiated THP1  (Rested) cells treated without or with 250 nM Torin-1  for 1 h."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Immunoblot  analysis of proteins from nuclear  and cytosolic  fractions from naïve THP1-WT or TFEB-S401A knock-ins (clones I11 and M17) cells treated with 50 ng/ml PMA  for the indicated times  and PMA-differentiated THP1  (Rested) cells."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Immunoblot  analysis of proteins from nuclear  and cytosolic  fractions from naïve THP1-WT or TFEB-S401A knock-in (clone I11) cells treated with 50 ng/ml PMA  for the indicated times."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Immunoblot  analysis of proteins from nuclear  fractions from naïve THP1-WT or TFEB-S401A knock-in (clone I11) cells treated with 50 ng/ml PMA or 250 nM  Torin-1 for 1 h. Immunoblots  are representative of at least three independent experiments."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Immunoblot  analysis of proteins from nuclear  and cytosolic  fractions from naïve THP1-WT or TFEB-S401A knock-in (clone I11) cells treated with 50 ng/ml PMA  for 6 h."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Immunoblot  analysis of protein lysates   and cell culture medium from PMA-differentiated (Rested) THP1-WT or TFEB-S401A knock-ins (clones I11 and M17) cells incubated with 0.1 μg/ml LPS  for 4 h prior to the addition of 15 μM Nigericin  for 45 min. C-F. Quantification of immunoblot  data shown in (B). Data are presented as mean ± SD using one-way ANOVA (unpaired) followed by Tukey's multiple comparisons test, (ns) not significant, (*)p&lt;0.05, (**)p&lt;0.01, (***)p&lt; 0.001 and (****)p&lt; 0.0001 from three independent experiments. Data information: n ≥ 3 biological replicates (each dot represents a biological replicate)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Fluorescence confocal microscopy of PMA-differentiated (Rested) THP1-WT or TFEB-S401A knock-ins (clones I11 and M17) cells incubated with 1 μg/ml LPS  for 6 h prior to the detection of dead cells with LIVE/DEAD fixable blue dead cell stain. Scale bars, 10 μm. Quantification of the  percentage of dead cells shown in (G). Data are presented as mean ± SD using one-way ANOVA (unpaired) followed by Tukey's multiple comparisons test, (ns) not significant and (****)p&lt; 0.0001 as compared to their corresponding untreated (Control) cells, with &gt;200 cells counted per trial from three independent experiments. Data information: n ≥ 3 biological replicates (each dot represents a biological replicate)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Double-immunolabeling of Sororin (green) and SYCP3 (red), and counterstaining of the chromatin with DAPI (blue) on spread Sgo2-/- spermatocytes at (A) pachytene (Pac.), (B) diplotene (Dip.), (C) metaphase I (M I), and (D) metaphase II-like (M II-like). The sex bivalent (XY) is indicated. Arrowheads indicate round bodies. Arrows in (A) indicate the pseudoautomal region in the sex bivalent, and the centriolar area at cell poles in (C,D). Scale bar: 10 µm."
NCBI gene IDs:
"Sgo2: 244495///68549"
Figure legend: "Double-immunolabeling of Sororin (green) and SYCP3 (red) on spread cultured wild-type control (WT Control), and okadaic acid treated wild-type (WT + OA) and Smc1β-/- (Smc1β-/- + OA) spermatocytes.A-B WT control cultured spermatocytes in (A) late zygotene (L. zyg.) and (B) metaphase I (M I). Arrows indicate the cell poles.C-D WT cultured spermatocytes treated with okadaic acid in (C) late zygotene (L. zyg.) and (D) metaphase I-like (M I-like).E-F Smc1β-/- cultured spermatocytes treated with okadaic acid in (E) pachytene-like (Pac.-like) and (F) metaphase I-like (M I-like). Arrowheads indicate nucleolar-associated round bodies. The unsynapsed axial elements of the sex chromosomes (X,Y) in prophase I, as well as the sex bivalent (XY) in metaphases I are indicated. Scale bar: 10 µm."
NCBI gene IDs:
"Smc1β: 140557"
Figure legend: "Co-staining of P28 and GAP45 (IMC protein) in female gametes (P28+ only) and zygotes (P28+/GAP45+) of the 17XNL, Δap2-o3 and Δcdpk4 strains. Percentages of female gametes and zygotes were indicated respectively. n is the number of cells counted. Scale bars = 5 μm. Note: the signal of P28 and GAP45 is reduced similarly in all Δap2-o3 female gametes and zygotes compared to that of 17XNL."
NCBI gene IDs:
"cdpk4: 3830518
ap2-o3: 3791637"
Figure legend: "Day 7 midgut oocyst counts from mosquitoes infected with parasites, including 17XNL, Δap2-o3, Δmap2, or Δnek4 strain alone as well as mixtures of Δmap2/Δnek4, Δap2-o3/Δmap2, and Δap2-o3/Δnek4. Δnek4 and Δmap2 are female and male gamete-defect parasites, respectively. n is the number of mosquitoes dissected, Mann-Whitney test applied, two experiments repeated."
NCBI gene IDs:
"nek4: 3829560
map2: 3791836
ap2-o3: 3791637"
Figure legend: "Transcript expression heatmap by RNA-seq of 26 genes involving DNA replication and DNA repair between DFsc7 (WT) and DFsc7;Δap2-o3 (KO) strains."
NCBI gene IDs:
"ap2-o3: 3791637"
Figure legend: "Representative fluorescence microscopy images of mCherry and DPOD2::GFP expression in female and male gametocytes of the DTS1 (ccp2::mCherry;dpod2::gfp) and DTS1;Δap2-o3 strains. mCherry is specifically expressed in female gametocytes. Flow cytometry detection of mCherry and DPOD2::GFP expression in female and male gametocytes of the DTS1 and DTS1;Δap2-o3 strains. The female and male gametocyte populations are circled by solid and dashed line respectively. E,F Similar analysis (as in C, D) in DTS2 (ccp2::mCherry;dpod1::gfp) and DTS2;Δap2-o3 strains. G,H Similar analysis (as in C, D) in DTS3 (ccp2::mCherry;rpa1::gfp) and DTS3;Δap2-o3 strains. I,J Similar analysis (as in C, D) in DTS4 (ccp2::mCherry;mcm7::gfp) and DTS4;Δap2-o3 strains."
NCBI gene IDs:
"ccp2: 
gfp: 
mCherry: 
dpod2: 3807075
rpa1: 3790834
dpod1: 3807142
mcm7: 3830453
ap2-o3: 3791637"
Figure legend: "EMSA using the recombinant GST-fused AP2 domain of AP2-O3 and a synthesized DNA probe containing three repeats of predicted motif. GST was used as a negative control. An arrowhead indicates the shifted band."
NCBI gene IDs:
"AP2-O3: "
Figure legend: "Western blot of GAP45 protein expression in non-activated (NAG) and activated gametocyte (AG) of 17XNL and Δap2-o3 strains. BiP as loading control."
NCBI gene IDs:
"ap2-o3: 3791637"
Figure legend: "IFA of AP2-O3 protein expression in activated gametocytes of Pccp2 strains."
NCBI gene IDs:
"ccp2: "
Figure legend: "(b) Estimation of gene editing at the Trim28 loci using NGS-sequencing of amplicons. Black bars indicate % of frameshift indels. Columns show an average of two biological replicates per guide RNA and error bars show mean ± SD."
NCBI gene IDs:
"Trim28: 21849"
Figure legend: " (c) Western blot confirmed the loss of Trim28 expression upon Trim28-KO in mouse NPCs. "
NCBI gene IDs:
"Trim28: 21849"
Figure legend: " (d) RNA-seq analysis of the expression of TE-families using TEtranscripts (e) The significantly upregulated TE families with a fold change larger than 0.5 upon Trim28-KO in mouse NPCs. The dashed line indicates significance. "
NCBI gene IDs:
"Trim28: 21849"
Figure legend: " (f) CUT&amp;RUN analysis of H3K9me3 in mouse NPCs of all full length MMERVK10Cs (right panel) RNA-seq analysis of the Trim28-KO and control samples, visualising full length MMERVK10C elements. The location of the full length MMERVK10Cs are indicated as a thick black line under each histogram. "
NCBI gene IDs:
"MMERVK10C: 
MMERVK10Cs: 
Trim28: 21849"
Figure legend: " (g) Example of transcriptional readthrough outside the full length MMERVK10Cs into a nearby gene. "
NCBI gene IDs:
"MMERVK10Cs: "
Figure legend: " (b) Estimation of gene editing at the Trim28 loci using NGS-sequencing of amplicons from DNA isolated from 50.000 RFP+ nuclei per animal. One animal per group was analyzed. Black bars indicate % of the detected indels that disrupted the frameshift. "
NCBI gene IDs:
"RFP: 
Trim28: 21849"