EMBO/SourceData huggingface dataset:
Figure legend: "F-H COS‐1 cells stably expressing GFP‐lact‐C2 were treated with ATP8A1 siRNA1 or EHD1 siRNA1 for 72 h and stained with recombinant 2xPH and anti‐GM130 antibody. The intensities of 2xPH staining and GFP‐lact‐C2 in RE area were measured. The intensity of 2xPH (G) and the ratio of 2xPH to GFP‐lact‐C2 intensities (H) are shown. In (H), data are normalized to that of control cells. Data represent mean ± SD and were analyzed with two‐tailed Student's t‐test (n &gt; 26 cells from three independent experiments). **P 0.01; ***P 0.001; NS, not significant. Scale bars, 10 μm."
NCBI gene IDs:
"ATP8A1: 10396
EHD1: 10938"
Figure legend: "D Cells were treated with ATP8A1 siRNA1 for 72 h. ATP8A2‐1D4 (WT or mutants) and myc‐CDC50A were transfected 48 h after siRNA transfection. The cells were then stained for EHD1 and 1D4‐tag. Scale bars, 10 μm."
NCBI gene IDs:
"ATP8A1: 10396
ATP8A2: 51761"
Figure legend: "F Cells were treated with ATP8A1 siRNA for 72 h. GFP‐ATP8A2 (WT or mutant) and myc‐CDC50A were transfected 48 h after siRNA transfection. The cells were then stained for TfnR. Data represent mean ± SD of three or four independent experiments. At least 20 cells were counted per experiment."
NCBI gene IDs:
"ATP8A1: 10396
ATP8A2: 51761"
Figure legend: "G, H Surface biotinylation assay with Atp8a2+/+ or Atp8a2−/− cortical neurons. The ratio of surface to total TfnR was quantified and normalized to Atp8a2+/+. Data represent mean value (2 mice for each genotype)."
NCBI gene IDs:
"Atp8a2: 50769"
Figure legend: "A, miR-203 expression, as determined by qPCR, in five temporal different stages of normal early development: oocyte, 2-cell embryo, morula, compacted morula and blastocyst. RNA was extracted from 30 different embryos and pooled in two independent groups for analysis by qPCR. RNA expression is normalized by a housekeeping miRNA (miR-16) that maintained invariable during early embryogenesis. Data represent the mean of 6 different qPCR measures (red bars). P=0.05 (Student's t-test) comparing 2C/morula versus compacted morula/blastocyst."
NCBI gene IDs:
"miR-16: 
miR-203: 387199"
Figure legend: "H, Representative images of EBs derived from human iPSCs transiently transfected with either control (left) or miR-203 mimics (right), at different time points during the differentiation process. Scale bars, 500 μm. I, Left panel shows the quantification of EBs size derived from human iPSCs transiently transfected with either control or miR-203 mimics as in panel (H) at different time points during the differentiation process. The percentage of EBs presenting internal large cavities during the indicated time course of differentiation is shown in the right panel. Data are mean ± s.e.m. (n=3 independent experiments). ***P&lt;0.001 (Student´s t-test). "
NCBI gene IDs:
"miR-203: 406986"
Figure legend: "B, C, Relative Luciferase Units (RLU; normalized to Renilla luciferase and relative to DNA amount) in 293T cells transfected with DNA constructs carrying the wild-type 3´UTRs from the indicated transcripts (B) or the mutated versions of Dnmt3a and Dnmt3b 3´UTRs, downstream of the luciferase reporter (C). Cells were co-transfected with Renilla luciferase as a control of transfection, and a plasmid expressing GFP or miR-203-GFP. Data are represented as mean ± s.d. (n=3 independent experiments)."
NCBI gene IDs:
"GFP: 
GFP.: 
Luciferase: 
luciferase: 
Dnmt3a: 13435
Dnmt3b: 13436
miR-203: 406986"
Figure legend: "E, Representative images of embryoid bodies (EBs) derived from wild-type iPSCs in which the expression of Dnmt3a and Dnmt3b was transiently repressed by siRNAs. Scale bars, 500 μm. Plots show the quantification of the size of EBs and the percentage of EBs with large cavities or beating at different time points during the differentiation process. Data are represented as mean ± s.e.m. (n=3 independent experiments)."
NCBI gene IDs:
"Dnmt3a: 13435
Dnmt3b: 13436"
Figure legend: "F, Expression levels of Dnmt3a or Dnmt3b transcripts after transfection of wild-type iPSCs with specific siRNAs either against Dnmt3a, Dnmt3b or a combination of both (Dnmt3a/b) (as indicated in E). RNA expression was measured 24 hours after the transfection protocols and was normalized by GAPDH mRNA levels. Data are represented as mean ± s.e.m. (n=3 independent experiments)."
NCBI gene IDs:
"GAPDH: 
Dnmt3a: 13435
Dnmt3b: 13436"
Figure legend: "G, Representative images of EBs derived from miiPSCs that were transiently and simultaneously transduced with Dnmt3a and Dnmt3b cDNAs or empty vectors, and simultaneously treated with Dox to induce miR-203 expression. Scale bars, 500 μm. Plots show the quantification of EB size, percentage of EBs with large cavities, and beating EBs at different time points during differentiation. Data are represented as mean ± s.e.m. (n=3 independent experiments)."
NCBI gene IDs:
"Dnmt3a: 13435
Dnmt3b: 13436
miR-203: 406986"
Figure legend: "H, Expression levels of miR-203, Dnmt3a or Dnmt3b transcripts in iPSCs after induction of miR-203 (miiPSCs) and transduced with Dnmt3a and Dnmt3b cDNAs or empty vectors (as indicated in G). RNA expression was measured 24 hours after the transfection protocols and was normalized by a control miRNA (miR-142) or GAPDH mRNA, respectively. Data are represented as mean ± s.e.m. (n=3 independent experiments)."
NCBI gene IDs:
"GAPDH: 
miR-142: 
Dnmt3a: 13435
Dnmt3b: 13436
miR-203: 406986"
Figure legend: "H, The specific differentially methylated regions (DMR) at the Sirt6 locus were analyzed by PCR amplification and sequencing of bisulphite-modified DNA. Ten independent clones were sequenced per condition. The quantification of methylated (filled circles) vs. unmethylated (empty circles) CpGs is shown in the histogram in the indicated conditions."
NCBI gene IDs:
"Sirt6: 50721"
Figure legend: "B, Representative immunofluorescences showing cardiac Troponin T (cTnT, green) and nuclei (Hoechst, blue) staining of in vitro-generated cardiomyocytes derived from wild-type iPSCs transiently transfected with either control mimics, miR-203 mimics or miR-203 mimics + Dnmt3a/b cDNAs. Pictures were taken at day 15 of differentiation. Scale bars, 68 μm. The cTnT-positive area in these cardiomyocytes is shown in the right histogram. Data are represented as mean ± s.d. (n=2 independent experiments with 6 replicates each)."
NCBI gene IDs:
"Dnmt3a: 13435
miR-203: 406986"
Figure legend: "Western blot showing STN1 knockdown. shLUC (siRNA targeting luciferase) was used as the control."
NCBI gene IDs:
"LUC: 
luciferase: 
STN1: 79991"
Figure legend: "CST deficiency causes ssDNA accumulation upon fork stalling. HeLa cells with STN1 knockdown were incubated with 10 µM BrdU for 48 h, followed by 2 mM HU treatment for 3 h, and subsequently stained with BrdU antibody under a non-denaturing condition to detect ssDNA. N, the number of cells analyzed in each condition. Relative BrdU fluorescence intensity was quantitated by Image J."
NCBI gene IDs:
"STN1: 79991"
Figure legend: "DNA fiber analysis of U2OS cells with STN1 knockdown and its RNAi-resistant Flag-STN1 WT re-expression with and without mirin treatment."
NCBI gene IDs:
"Flag: 
STN1: 79991"
Figure legend: "DNA fiber analysis of BJ cells with STN1 knockdown by two siRNA sequence with and without mirin treatment."
NCBI gene IDs:
"STN1: 79991"
Figure legend: "DNA fiber analysis of HCT116 cells with STN1 knockdown with and without mirin treatment."
NCBI gene IDs:
"STN1: 79991"
Figure legend: "DNA fiber analysis of U2OS cells showing that SMARCAL1-mediated fork reversal is needed for nascent-stand degradation observed in CST-deficient cells."
NCBI gene IDs:
"SMARCAL1: 50485"
Figure legend: "DNA fiber analysis of U2OS cells showing that ZRANB3-mediated fork reversal is needed for nascent-strand degradation observed in CST-deficient cells."
NCBI gene IDs:
"ZRANB3: 84083"
Figure legend: "SIRF detection of MRE11 at stalled forks in STN1-deficient U2OS cells. Representative SIRF images of MRE11 at normal or stalled replication forks are shown. Scale bars: 10 µm."
NCBI gene IDs:
"STN1: 79991"
Figure legend: "ssDNA accumulation analysis in STN1-deficient U2OS cells with and without mirin treatment. Representative images of native BrdU staining are shown."
NCBI gene IDs:
"STN1: 79991"
Figure legend: "Coomassie blue stained SDS-PAGE gel (15%) of purified human CST complex (CTC1 wild- type and ∆700N)."
NCBI gene IDs:
"CTC1: 80169"
Figure legend: "Deletion of the N-terminal 700 aa of CTC1 abolishes CTC1 localization at telomeres. U2OS stably expressing vector (V), WT Myc-CTC1 and Myc-∆700N were treated with or without HU and co-stained with Myc (red) and TRF2 (green) antibodies. Boxed areas are amplified in inserts to indicate CTC1/TRF2 colocalization (yellow).Scale bars: 10 μm. Quantification of percent of cells with &gt;3 CTC1/TRF2 colocalization foci were from three independent experiments. In each experiment, &gt;150 cells from each sample were analyzed."
NCBI gene IDs:
"Myc: 
CTC1: 80169"
Figure legend: "   ∆700N does not affect CST complex formation. HEK293T cells were co-transfected with Myc- CTC1 or Myc-∆700N, His6-STN1 and HA-TEN1. Co-IP was performed with Myc antibody to pulldown His6- STN1 and HA-TEN1. Full-length Myc-CTC1 was prone to degradation during immunoprecipitation.   "
NCBI gene IDs:
"HA: 
His: 
Myc: 
STN1: 79991
CTC1: 80169
TEN1: 100134934"
Figure legend: "   ∆700N retains RAD51 interaction. HEK293T cells were co-transfected with Myc-CTC1 or Myc-∆700N, His6- STN1, HA-TEN1 and Flag-RAD51 or vector control (V), and treated with HU (2 mM, 16 h). Co-IP was performed with Flag antibody to pulldown Myc-CTC1.   "
NCBI gene IDs:
"Flag: 
HA: 
His: 
Myc: 
STN1: 79991
CTC1: 80169
RAD51: 5888
TEN1: 100134934"
Figure legend: "DNA fiber analysis of CTC1 depleted U2OS cells with RNAi-resistant WT or ∆700N re-expression. Mean values in each sample are listed at the top of the graph. N, the number of cells analyzed in each condition, ***"
NCBI gene IDs:
"CTC1: 80169"
Figure legend: "Micronuclei (MN) formation in BRCA2- and STN1-deficient cells. Co-depletion was achieved by transfecting siBRCA2 in U2OS cells expressing shSTN1. Representative DAPI staining images are shown. Arrows point to MN. Scale bars: 20 µm. Average percentages of nuclei containing MN from three independent experiments are presented. In each experiment, >300 nuclei were analyzed per sample."
NCBI gene IDs:
"BRCA2: 675
STN1: 79991"
Figure legend: "Anaphase bridges (arrows) in BRCA2- and STN1-deficient U2OS cells. Scale bar: 10 µm. Average percentages of anaphase bridges from three independent experiments are presented."
NCBI gene IDs:
"BRCA2: 675
STN1: 79991"
Figure legend: "   γH2AX induced by BRCA2 knockdown and STN1 knockdown in U2OS cells. Nuclei containing ≥ 5 foci were considered as positive γ-H2AX staining. Results were from three independent knockdown experiments. In each experiment, &gt;80 cells were analyzed per sample.   "
NCBI gene IDs:
"BRCA2: 675
STN1: 79991"
Figure legend: "Co-depletion of STN1 and BRCA2 significantly impairs DNA replication. Scale bar: 50 µm. Results were from three independent knockdown experiments. In each experiment, &gt;180 nuclei were analyzed per sample."
NCBI gene IDs:
"BRCA2: 675
STN1: 79991"
Figure legend: "Co-depletion of STN1 and BRCA2 increases chromosome instabilities. U2OS cells with siBRCA2 and/or shSTN1 knockdown were treated with HU (2 mM, 3 h). Representative metaphase images show aberrant chromosomes (red arrows). Boxed areas are amplified and shown at the bottom of images. Scale bars: 20 µm. Two independent knockdown and chromosome spread experiments were performed. N, the number of metaphase spreads analyzed in each sample."
NCBI gene IDs:
"BRCA2: 675
STN1: 79991"
Figure legend: "RAD51 foci formation in U2OS CTC1 knockdown cells was rescued by RNAi-resistant WT but not by ∆700N. Cells were treated with HU (2 mM, 16 h) and stained with anti-RAD51. Representative images are shown. Scale bars: 10 µm. Scatter plot of RAD51 foci number with and without HU treatment is shown. Mean values in each condition are listed at the top of the graph."
NCBI gene IDs:
"CTC1: 80169"
Figure legend: "DNA fiber analysis of nascent-strand DNA degradation in U2OS cells with STN1 and RAD51 co-depletion. Three independent treatments were performed and results from one biological replicate are shown. N, the number of DNA fibers analyzed in each sample in one experiment. Western blots showing protein knockdown in DNA fiber assays are included."
NCBI gene IDs:
"RAD51: 5888
STN1: 79991"
Figure legend: "A. Raw 264.7 macrophage cells were treated with DMSO (0.05%), 1 μM control antisense oligonucleotide (ASO), or Rab10-directed ASO (1 μM), for four days. Immunoblots are representative of three independent experiments, with similar results obtained. B. Calculated reduction of Rab10 (n=3 biologically independent experiments). Each dot in the plot presents one independent experiment. Group are shown. Error bars represent ± SEM. Significance was assessed by one-way ANOVA with Tukey's post hoc test, with **** representing P &lt;0.0001."
NCBI gene IDs:
"Rab10: 19325"
Figure legend: "A. Raw 264.7 macrophage cells were transfected with FLAG(N-term)-Rab10 and eGFP(C-term)-Akt-PH domain (PI(3,4,5)P3 marker), or eGFP(C-term)-SARA-Fyve domain (PI3P marker), or eGFP(C-term)-PLC-PH domain (PI(4,5)P2 marker), or mkate2(N-term)-2xTAPP1-PH domain (PI(3,4)P2 marker). Representative photomicrographs (from 25 images analyzed for each condition from n=3 biologically independent experiments) are shown from fixed cells immuno-stained for FLAG-tag (Rab10, shown as green) together with eGFP epifluorescence (shown as red). White bounding boxes show \"High Mag\" panels that magnify representative individual vesicles. The colocalization of Rab10 and indicated markers in the bounding boxes were analyzed using Image J as described in the method and shown in the \"CoLoc\" panels. The colocalized pixels are shown in white with the same intensity. Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panels and \"CoLoc\" panels. White arrow heads indicate representative Rab10 vesicles positively colocalized with the indicated marker. B. At least 25 cells across three independent experiments were analyzed for each condition (see Materials and Methods section). % of Rab10 vesicles positive with the indicated marker within each cell was quantified. Each dot represents the mean value from one cell analyzed. Group means are given, with error bars representing ± SEM. "
NCBI gene IDs:
"eGFP: 
FLAG: 
mkate2: 
Akt: 11651
PLC: 5333
TAPP1: 59338
Rab10: 10890
SARA: 51128"
Figure legend: "C. Raw 264.7 macrophage cells were transfected with FLAG(N-term)-Rab10 and eGFP(C-term)-Akt-PH domain (PI(3,4,5)P3 marker) and treated with wortmannin (1 μM) for one hour. Representative photomicrographs (from 25 cells analyzed for each condition from n=3 biologically independent experiments) are shown from fixed cells immuno-stained for FLAG-tag (Rab10, shown as green) together with eGFP epifluorescence (shown as red). Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panel. D. Percent of cells treated with or without wortmannin harboring Rab10 vesicles was calculated from &gt;30 cells across five independent experiments. Each dot on the plot represents mean values from one experiment. Error bars represent ± SEM and significance was assessed by Mann-Whitney test (owing to non-normal distributions) with ** indicating P&lt;0.01. "
NCBI gene IDs:
"eGFP: 
FLAG: 
Akt: 11651
Rab10: 10890"
Figure legend: "E. Raw 264.7 cells were co-transfected with eGFP(N-term)-Rab10 and mRuby(N-term)-Rab5 for 48 hours, with live-cell recordings for ~10 minutes. A representative vesicle is shown over the presented time-lapse. Scale bar represents 1 μm. F. Fluorescence intensities of individual vesicles are calculated over time. Dots show mean values calculated from n=8 recorded vesicles from cells from n=3 biologically independent experiments. Error bars show ± SEM. "
NCBI gene IDs:
"eGFP: 
mRuby: 
Rab10: 10890
Rab5: 5868"
Figure legend: "Raw264.7 cells were transfected with plasmids expressing eGFP(N-term)-WT-Rab10 or Q68L-Rab10 (GTP-locked). 24-hours later, cells were incubated with (A) TRITC-dextran (70 kDa, shown as red signal) Representative photomicrographs are from 25 cells each analyzed for each condition from n=3 biologically independent experiments. White bounding boxes are magnified in \"High Mag\" panels that show individual vesicles. Positive co-localization signal (see Methods) is indicated in bounding boxes labeled as \"CoLoc.\" Colocalized pixels are shown in white. Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panels and \"CoLoc\" panels. White arrow heads indicate Rab10 vesicles colocalized with the indicated marker."
NCBI gene IDs:
"eGFP: 
Rab10: 10890"
Figure legend: "Raw264.7 cells were transfected with plasmids expressing eGFP(N-term)-WT-Rab10 or Q68L-Rab10 (GTP-locked). 24-hours later, cells were incubated with (B) DQ-ovalbumin, for 30 min before washing and fixation. Representative photomicrographs are from 25 cells each analyzed for each condition from n=3 biologically independent experiments. White bounding boxes are magnified in \"High Mag\" panels that show individual vesicles. Positive co-localization signal (see Methods) is indicated in bounding boxes labeled as \"CoLoc.\" Colocalized pixels are shown in white. Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panels and \"CoLoc\" panels. White arrow heads indicate Rab10 vesicles colocalized with the indicated marker."
NCBI gene IDs:
"eGFP: 
Rab10: 10890"
Figure legend: "Raw264.7 cells were transfected with plasmids expressing eGFP(N-term)-WT-Rab10 or Q68L-Rab10 (GTP-locked). (C, D) Cells were further stained for (C) EEA1, or (D) Lamp1, which was detected with Cy5 dye (show as red signal). Representative photomicrographs are from 25 cells each analyzed for each condition from n=3 biologically independent experiments. White bounding boxes are magnified in \"High Mag\" panels that show individual vesicles. Positive co-localization signal (see Methods) is indicated in bounding boxes labeled as \"CoLoc.\" Colocalized pixels are shown in white. Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panels and \"CoLoc\" panels. White arrow heads indicate Rab10 vesicles colocalized with the indicated marker."
NCBI gene IDs:
"eGFP: 
Rab10: 10890"
Figure legend: "A. Raw 264.7 macrophage cells were transfected with Flag(N-term)-Rab10 and eGFP(C-term)-Akt-PH domain(PI(3,4,5)P3 marker), or eGFP(C-term)-SARA-Fyve domain((PI3P marker), or eGFP(C-term)-PLC-PH domain (PI(4,5)P2 marker, epifluorescence, shown in blue) followed by anti-Flag immunofluorescence (shown in green) and pT73-Rab10 antibody (shown in red). To evaluate colocalization of phospho-Rab10 with early endosomes or late endosome/lysosome markers, immunofluorescence detection was performed for endogenous EEA1 or Lamp1 (shown in blue). Raw 264.7 macrophage cells were further fed 0.05 mg mL-1 of 70 kDa TRITC-conjugated dextran (epifluorescence, shown in blue) or 0.05 mg mL-1 BODIPY conjugated ovalbumin for 30min prior to immunofluorescence analysis. Representative images were selected from 25 cells analyzed for each condition from n=3 biologically independent experiments. White bounding boxes are \"High Mag\" panels that show individual vesicles analyzed. Scale bars represent 10 μm and 1 μm for \"High Mag\". Positive colocalization signal (depicted as white) in the \"CoLoc (pRab10/Rab10)' and 'CoLoc (pRab10/Marker)' columns are indicated. White arrow heads highlight vesicles scored as co-positive between pT73-Rab10 and Rab10 or the indicated marker. B. 25 cells across three independent experiments were analyzed for each condition, with each dot representing the mean of the vesicle analysis across individual cells. The group means of %pT73-Rab10 vesicles positive for the indicated marker are given, with error bars showing ± SEM. "
NCBI gene IDs:
"eGFP: 
Flag: 
Akt: 11651
PLC: 5333
Rab10: 10890
SARA: 51128"
Figure legend: "A. Raw264.7 cells were transfected with Flag(N-term)-LRRK2 and eGFP(N-term)-Rab10 for 24 hours and treated with or without the LRRK2 kinase inhibitor MLi2 (100 nM) for two hours. Representative photomicrographs (from &gt;20 images analyzed for each condition from n=3 biologically independent experiments) are shown from fixed cells with eGFP(N-term)-Rab10 epifluorescence (shown as green signal), and immuno-stained for pT73-Rab10 (shown as red signal) and LRRK2 (shown as blue signal). White bounding boxes are magnified in \"High Mag\" panels that show individual vesicles. Scale bars represent 10 μm or 1 μm in \"Hig Mag\" panels."
NCBI gene IDs:
"eGFP: 
Flag: 
LRRK2: 120892
Rab10: 10890"
Figure legend: "B. Raw 264.7 macrophage cells transfected with eGFP(N-term)-Rab10 and Flag(N-term)-LRRK2 were sequentially lysed mechanically into buffer to create a 'soluble' protein fraction, and then insoluble material lysed into triton X-100 buffer to create a \"triton x-100\" fraction. The \"soluble\" and triton X-100 buffer solubilized fractions were analyzed using SDS-PAGE (TGX gels), and representative immunoblots from n=3 independent experiments are shown."
NCBI gene IDs:
"eGFP: 
Flag: 
LRRK2: 120892
Rab10: 10890"
Figure legend: "A,B. Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male WT-LRRK2 mBAC transgenic mice were pre-incubated with FITC-conjugated dextran (70 kDa, green signal) prior to washing, fixing, and immunostaining with total-Rab10 (shown as red signal), or pT73-Rab10 antibody (shown as red signal). Alternatively, cells were co-stained with antibodies to Rab5, Rab7 or Lamp1 (shown as green signal in separate images). Representative photomicrographs (from &gt;30 images analyzed for each condition from n=3 biologically independent experiments) are shown. White bounding boxes are \"High Mag\" panels that show individual vesicles analyzed. Positive colocalization signal (depicted as white) in \"CoLoc\" panels are given with white arrow heads highlighting vesicles scored as co-positive between pT73-Rab10 and the indicated marker. Scale bars are 10 μm, or 1 μm in \"Hig Mag\" panels and \"CoLoc\" panels."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "D. Primary mouse bone marrow-derived macrophage cells (BMDM) from adult male WT-LRRK2 mBAC transgenic mice were treated with 0.001% DMSO or 100 nM MLi2 (i.e., IC90 concentration) for two hours prior to immunostaining with total Rab10 or pT73-Rab10 antibody (shown as red signal), and anti-Rab5 or anti-Rab7antibody (shown as green signal). Representative photomicrographs (from 20 cells analyzed for each condition from n=3 biologically independent experiments) are shown. White bounding boxes are \"High Mag\" panels that show individual vesicles analyzed. Scale bars represent 10 μm and 1 μm for \"High Mag\". Positive colocalization signal (depicted as white) in \"CoLoc\" panels are given with white arrow heads highlighting vesicles scored as co-positive between pT73-Rab10 and the indicated marker."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "F. Raw 264.7 macrophage cells were transfected with eGFP(N-term)-Rab10 for 24 hours and treated with or without the LRRK2 kinase inhibitor MLi2 (100 nM) for two hours. Representative photomicrographs (from 25 images analyzed for each condition across n=3 biologically independent experiments) are shown with eGFP(N-term)-Rab10 epifluorescence (shown as green signal) and pT73-Rab10 (shown as red signal), with EEA1 (shown as blue signal). White bounding boxes are \"High Mag\" panels that show individual vesicles analyzed. Scale bars represent 10 μm and 1 μm for \"High Mag\". Positive colocalization signal (depicted as white) in \"CoLoc\" panels are given with white arrow heads highlighting vesicles scored as co-positive between pT73-Rab10 Rab10 and the indicated marker. G. Calculated percentage of colocalized vesicles, with each dot representing mean vesicle frequency in each cell analyzed. The group mean of % Rab10 vesicles positive with EEA1 were calculated, and error bars represent ± SEM. Significance was assessed by Mann-Whitney test for each marker with *** representing P&lt;0.005. "
NCBI gene IDs:
"eGFP: 
Rab10: 10890"