EMBO/SourceData huggingface dataset:
Figure legend: "(a) Intracellular spermidine of five-day-oldΔspe1 cells, compared with wild-type cells. Data represent means ± s.e.m. (n = 3; *P 0.001)."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(b) Chronological ageing of wild-type (▪, o) and polyamine-depleted Δspe1 (▴, ▿) yeast cells with (open symbols) and without (closed symbols) supplementation of low doses of spermidine. Data represent means ± s.e.m. (n = 4). Cells were tested for cell death markers at day 3."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(c) Fluorescence microscopy of DHE→Eth conversion in wild-type and Δspe1 cells indicating ROS production. Scale bars, 10 μm."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(d) Quantification (FACS analysis) of ROS accumulation using DHE→Eth conversion of wild-type (WT) and Δspe1 cells with and without supplementation of low doses of spermidine. Data represent means ± s.e.m. (n = 4; *P 0.001)."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(e) Quantification (FACS analysis) of phosphatidylserine externalization and loss of membrane integrity using annexin V/PI co-staining (at day 5) and of DNA fragmentation using TUNEL staining (at day 3) of chronologically ageing wild-type (WT) and Δspe1 cells with or without supplementation of 0.1 mM spermidine as indicated. Data represent means ± s.e.m. (n = 3; *P 0.001)."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(e) Fluorescence microscopy of chronologically aged wild-type cells (day 3 and 14) expressing an EGFP-tagged version of the yeast HMGB1 homologue (Nhp6A-EGFP) with or without (control) addition of 4 mM spermidine. Scale bars, 5 μm."
NCBI gene IDs:
"HMGB1: 3146
Nhp6A: 856165"
Figure legend: "(c) Relative acetylation (normalized to wild-type controls at each day) of indicated histone H3 Lys residues of Δspe1 cells (open bars), compared with wild-type cells (closed bars) during chronological ageing. Data represent means ± s.e.m. (n = 3). P values indicate the result of a two-factor ANOVA corrected by Bonferroni post hoc test."
NCBI gene IDs:
"spe1: 853651"
Figure legend: "(a) Relative acetylation of histone H3 Lys 9 and 14 residues determined by quantification of immunoblot analysis performed at day 20 of the ageing experiment shown in panel b. Data represent means ± s.e.m. (n = 3; *P 0.05). (b) Chronological ageing of wild-type (▪, o) and Δiki3Δsas3 (▴, ▿) with (open symbols) or without (closed symbols) addition of spermidine (4 mM) at day 1. Data represent means ± s.e.m. (n = 4). (c) Quantification (FACS analysis) of phosphatidylserine externalization and loss of membrane integrity (annexinV/PI costaining) as well as ROS production (DHE-&gt;Eth conversion) performed at day 20 of the experiment shown in b. Data represent means ± s.e.m. (n = 4; *P 0.001)."
NCBI gene IDs:
"iki3: 851100
sas3: 852228"
Figure legend: "(g) Relative change of ATG7, ATG11 and ATG15 mRNA levels by spermidine supplementation (normalised to controls) after ten days of chronological ageing as determined by reverse transcriptase real-time PCR. Data represent means ± s.e.m. (n = 3; *P 0.05 and **P 0.01)."
NCBI gene IDs:
"ATG11: 856162
ATG15: 850432
ATG7: 856576"
Figure legend: "(h) Relative histone H3 Lys 18 acetylation of indicated promoter regions normalized to the acetylation of ATG7 promoter (pATG7) determined by chromatin immunoprecipitation with H3-K18Ac specific antibody and subsequent quantification of precipitated promoter DNA using quantitative real time PCR. Signals specific for indicated promoter regions were initially compared with non-immunoprecipitated samples and afterwards normalized to the pATG7 signals. Data represent means ± s.e.m. (n = 3). The P value was calculated using a two-factor ANOVA with promoter and condition (treated and non-treated) as independent factors."
NCBI gene IDs:
"ATG7: "
Figure legend: "(a) Fluorescence microscopy of wild-type yeast cells expressing an EGFP-Atg8p fusion protein with or without (control) treatment of 4 mM spermidine for 48 h. White arrows indicate vacuolar localization of EGFP-Atg8p indicative of autophagy. Scale bars, 5 μm."
NCBI gene IDs:
"Atg8p: 852200"
Figure legend: "(b) Relative alkaline phosphatase activity (ALP activity) indicative of autophagy during chronological ageing of pho8ΔC60 yeast with (open bars) or without (closed bars) application of spermidine (4 mM). Data represent means ± s.e.m. (n = 3 *; P 0.01 and **P 0.001)."
NCBI gene IDs:
"pho8: 852092"
Figure legend: "(c) Relative alkaline phosphatase activity (ALP activity) indicative of autophagy during chronological ageing of wild-type (closed bars) and Δiki3Δsas3 (open bars) cells. Data represent means ± s.e.m. (n = 3; *P 0.01 and **P 0.001)."
NCBI gene IDs:
"iki3: 851100
sas3: 852228"
Figure legend: "(d) Chronological ageing of wild-type (▪, o) and Δatg7 (▴, ▿) with (open symbols) or without (closed symbols) addition of spermidine (4 mM) at day 1. Data represent means ± s.e.m. (n = 4)."
NCBI gene IDs:
"atg7: 856576"
Figure legend: "(e) Chronological ageing on water of wild-type (▪, o) and Δatg7 (▴, ▿) with (open symbols) or without (closed symbols) addition of spermidine (8 mM) at day 0. Data represent means ± s.e.m. (n = 4)."
NCBI gene IDs:
"atg7: 856576"
Figure legend: "(f) Quantification (fluorescence reader) of ROS production (DHE→Eth conversion) by wild-type and Δatg7 cells with and without supplementation of spermidine (8 mM), obtained from the ageing experiment shown in panel e. Data represent means ± s.e.m. (n = 4; *P 0.07 and **P 0.01)."
NCBI gene IDs:
"atg7: 856576"
Figure legend: "(a) Fluorescence microscopy of Hoechst-counterstained HeLa cells transiently transfected with LC3-GFP subjected to 100 μM spermidine for 6 h. Representative pictures are shown. (b) Percentage of adherent cells exhibiting a clear LC3-GFP relocalization into cytoplasmic vacuoles. Numbers were determined using micrographs of Hoechst-counterstained HeLa cells as representatively shown in a. Data represent means ± s.d. (n = 3)."
NCBI gene IDs:
"LC3: 440738///81631///84557"
Figure legend: "(e, f) Survival of Drosophila during ageing without (control) and with supplementation of food at various concentrations of spermidine (as indicated). Autophagy-deficient flies (f) homozygous mutant for Atg7 (Δatg7) were compared with flies capable of autophagy (e) and heterozygous for Atg7 (control). For details of strains and additional wild-type controls, see Supplementary Information Methods and Fig. S6h, i."
NCBI gene IDs:
"Atg7: 37141"
Figure legend: "(g) Fluorescence microscopy of C. elegans transgenic embryos expressing a full-length plgg-1DsRED::LGG-1 fusion protein indicative of autophagic activity. Shown are two representative pictures of embryos untreated (control) or treated with spermidine (0.2 mM) supplementation of food. (h) Quantification of autophagic activity through measurement of DsRED::LGG-1 pixel intensity from images of wild-type animals shown in g, and bec-1 RNAi knockdown animals (bec-1 RNAi). Data represent means ± s.e.m. (n = 3) with at least 25 images processed for each trial."
NCBI gene IDs:
"bec-1: 177345
lgg-1: 174050"
Figure legend: "(i) Survival of C. elegans during ageing with and without (control) supplementation of food (UV-killed E. coli) with spermidine (0.2 mM). Wild-type (N2) animals were compared with bec-1 RNAi animals deficient in autophagy induction. For mean life spans see Supplementary Information, Table S3."
NCBI gene IDs:
"bec-1: 177345"
Figure legend: "(E) Relative percentage of TNTs in cells containing α-synucleinfibrils untransfected (control) or transfected with Myosin 10 (Myosin 10). Overexpression of Myosin 10 in the donor cell population led to an increase in the number of TNTs."
NCBI gene IDs:
"Myosin 10: 17909"
Figure legend: "(F) Quantification of the average number of ATTO-550α-synucleinfibrils per acceptor cell after 24 hours co-culture with donor cells containing α-synucleinfibrils untransfected (control) or transfected with Myosin 10 (Myosin 10). Note that Myosin 10 overexpression increases both the transfer of DiIvesicles and the number of transferred α-synuclein puncta in acceptor cells; n = 3 independent experiments (**, p &lt; 0.01 by two-tailed Mann Whitney test)."
NCBI gene IDs:
"Myosin 10: 17909"
Figure legend: "(B) The ratio of unique histone iCLIP-seq read population to unique histone RNA-seq read population was calculated and shown, with the unique histone RNA-seq read population set as &quot;1&quot;. Error bars represent standard deviations from three replicates of ALYREF iCLIP data. Statistical analysis was performed using Student's t-test. ***P&lt; 0.001."
NCBI gene IDs:
"histone: 653604///8370///317772///440689///85235///8968///8970///8294///8329///3009///3024///3013///3017///8359///8355///8354///8353///8352///8350///8338///8336///8335///8334///8332///8331///8330///8362///8366///8364///8363///8365///8368///8367///8348///8347///8340///8341///8342///333932///85236///3008///8351///8339///8356///3006///8345///8358///8337///8349///8969///3012///8357///121504///8290///128312///92815
histone: 653604///8370///317772///440689///85235///8968///8970///8294///8329///3009///3024///3013///3017///8359///8355///8354///8353///8352///8350///8338///8336///8335///8334///8332///8331///8330///8362///8366///8364///8363///8365///8368///8367///8348///8347///8340///8341///8342///333932///85236///3008///8351///8339///8356///8345///3006///8358///8337///8349///8357///8969///3012///8290///121504///128312///92815
histone: 653604///8370///317772///440689///85235///8968///8970///8294///8329///3009///3024///3013///3017///8359///8355///8354///8353///8352///8350///8338///8336///8335///8334///8332///8331///8330///8362///8366///8364///8363///8365///8368///8367///8348///8347///8340///8341///8342///333932///85236///3008///8351///8356///3006///8345///8358///8337///8349///8969///3012///8357///8290///121504///8339///128312///92815"
Figure legend: "(D) The distribution of histone reads in 5' UTR, CDS and 3' UTR in rRNA-depleted nuclear RNA-seq and ALYREF-iCLIP-seq."
NCBI gene IDs:
"histone: 8356///3006///8345///8358///8969///8337///8349///3012///8357"
Figure legend: "(E) Metagene analysis of normalized ALYREF signals on histone mRNA based on iCLIP-seq data. iCLIP-seq signal at each position of an mRNA is divided by the sum of signal of the mRNA to normalize each mRNA's contribution to the plot."
NCBI gene IDs:
"histone: 8356///3006///8345///8969///8349///8337///8358///8357///3012"
Figure legend: "(G) Distribution profile of clustered ALYREF binding sites on histone mRNA 3' UTRs relative to the cleavage site (CS). The CS is set as &quot;0&quot; in the x axis, and the relative position to the CS is marked. The y axis shows the total occurrence of binding sites in three repeats at each nucleotide."
NCBI gene IDs:
"histone: 8356///3006///8345///8337///8969///8357///3012///8349///8358"
Figure legend: "(E) (Top) Domain schematic representation of SLBP. (Bottom) Flag IPs from RNase A-treated HeLa cell lysate individually expressing indicated Flag tagged proteins, followed by western blotting using Flag and ALYREF antibodies. 3% of input was loaded. The * indicates a band probably resulted from degradation of Flag-SLBP. The white line delineates the boundary where irrelevant lanes have been removed from the same blot"
NCBI gene IDs:
"Flag: 
SLBP: 7884"
Figure legend: "(E) (Top) Domain schematic representation of SLBP. followed by western blotting using Flag and ALYREF antibodies. 3% of input was loaded. The * indicates a band probably resulted from degradation of Flag-SLBP. The white line delineates the boundary where irrelevant lanes have been removed from the same blot except that Flag IPs were carried out from HA-SLBP stable expression cells transfected with plasmids expressing ALYREF fragments."
NCBI gene IDs:
"SLBP: 7884"
Figure legend: "(G) Western blotting to examine the KD efficiency of SLBP. GAPDH was used as a loading control. The white line delineates the boundary where irrelevant lanes have been removed from the same blot"
NCBI gene IDs:
"SLBP: 7884"
Figure legend: "Cntl- or SLBP-siRNA treated HeLa cells were used for IPs with IgG or the ALYREF antibody. The immunoprecipitates were subjected to western analysis (H) Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001, n.s., not significant."
NCBI gene IDs:
"SLBP: 7884"
Figure legend: "Cntl- or SLBP-siRNA treated HeLa cells were used for IPs with IgG or the ALYREF antibody. The immunoprecipitates were subjected to RT-qPCRs (I). Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001, n.s., not significant."
NCBI gene IDs:
"SLBP: 7884"
Figure legend: "(J) Metagene analysis of normalized ALYREF signals on histone mRNAs in Cntl and SLBP KD cells based on iCLIP-seq data. iCLIP-seq signal at each position of an mRNA is divided by the sum of signal of the mRNA to normalize each mRNA's contribution to the plot."
NCBI gene IDs:
"histone: 8356///8345///3006///8349///8358///8969///8337///8357///3012
SLBP: 7884"
Figure legend: "(B) List of histone genes with defective 3'-end processing detected by polyA+ and polyA- RNA-seq. PolyA+ ALYREF/Cntl shows polyA+ RNA-seq RPM ratio of each histone gene in ALYREF KD versus Cntl."
NCBI gene IDs:
"ALYREF: 10189"
Figure legend: "(E, F) RT-qPCRs to detect histone mRNA levels in the polyA+ (E) or the polyA- fraction (F) in cells treated with Cntl, ALYREF or ARS2 siRNA. The arrowhead and arrows indicate the cleavage site and primer location, respectively. The bars show the relative abundance of polyA+ forms to the actin mRNA (E) and that of polyA- forms relative to the 18S rRNA (F). Data information Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"actin: 
ALYREF: 10189
ARS2: 51593"
Figure legend: "Western blotting to examine ALYREF protein level (G) in Flag-Cntl (eIF4A3) or siRNA-resistant Flag-ALYREF stable expression cells treated with Cntl or ALYREF siRNA. The white line delineates the boundary where irrelevant lanes have been removed from the same blot"
NCBI gene IDs:
"ALYREF: 10189"
Figure legend: "RT-qPCRs to detect polyA+ histone mRNA levels (H) in Flag-Cntl (eIF4A3) or siRNA-resistant Flag-ALYREF stable expression cells treated with Cntl or ALYREF siRNA. The bars show the relative abundance of histone mRNAs to the GAPDH mRNA. Data information: Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"ALYREF: 10189
GAPDH: 2597"
Figure legend: "Cntl or ALYREF siRNA treated Flag-Lsm11 stable expression cells were used for IPs with IgG or the Flag antibody. The immunoprecipitates were subjected western analysis (D). * indicates the antibody heavy chain."
NCBI gene IDs:
"ALYREF: 10189"
Figure legend: "Cntl or ALYREF siRNA treated Flag-Lsm11 stable expression cells were used for IPs with IgG or the Flag antibody. The immunoprecipitates were subjected to RT-qPCRs (E) * indicates the antibody heavy chain. The bars show the relative IP efficiencies of histone pre-mRNAs to U7 snRNA. Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"ALYREF: 10189
U7 snRNA: 134353"
Figure legend: "(A) (Top) Illustration of the HIST2H2AA3 reporter construct. (Bottom) FISH to detect the distribution of the HIST2H2AA3 mRNA in HeLa cells depleted of Cntl, ALYREF, THOC2 (THO) and UAP56. DAPI staining served as a nuclear marker. N and C indicate nuclear and cytoplasmic FISH signals, respectively. N/C ratios were determined for 20 cells in each experiment. Data information: Error bars represent standard deviations from biological repeats (n=3 Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"ALYREF: 10189
UAP56: 10212
HIST2H2AA3: 8337
THO: 57187
THOC2: 57187"
Figure legend: "(B) Distribution of the endogenous HIST2H2AA3 mRNA was detected with the transcript-specific probe in HeLa cells depleted of Cntl, ALYREF, THOC2 (THO) and UAP56. N and C indicate nuclear and cytoplasmic FISH signals, respectively. N/C ratios were calculated Data information: Error bars represent standard deviations from biological repeats (n=3 Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"ALYREF: 10189
UAP56: 10212
HIST2H2AA3: 8337
THO: 57187
THOC2: 57187"
Figure legend: "Western analysis to examine the purities of nuclear and cytoplasmic fractions (C) in Cntl and UAP56 KD cells. MTR4 and Tubulin were used as the nuclear and cytoplasmic marker, respectively."
NCBI gene IDs:
"UAP56: 10212"
Figure legend: "RT-qPCRs to examine N/C distribution of each histone mRNA (D) in Cntl and UAP56 KD cells. Data information: Error bars represent standard deviations from biological repeats (n=3 Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"UAP56: 10212"
Figure legend: "(F) Relative NXF1 iCLIP read populations on histone mRNAs in Cntl, ALYREF and SLBP KD cells. Data information: Error bars represent standard deviations from biological repeats n=2 Statistical analysis was performed using Student's t-test. *P &lt; 0.05, **P &lt; 0.01, ***P&lt; 0.001."
NCBI gene IDs:
"ALYREF: 10189
histone: 8969///8356///8349///8337///8358///8357///8345///3012///3006
SLBP: 7884"
Figure legend: "(G) NXF1 iCLIP signals along the histone mRNA. The histone mRNA is divided into 10 fractions based on their mRNA size. Each fraction represents 10% of each histone mRNA. The tenth fractions that contains the SL is boxed by green dashed lines."
NCBI gene IDs:
"histone: 8969///8356///8349///8337///8358///8357///8345///3012///3006"
Figure legend: "(A) (Top) Illustration of the reporter constructs. The black and red lines indicate H2AA3-SL and H2AA3-SLD PCR products, respectively. The dashed line indicates the FISH probe. (Bottom) FISH to detect the distribution of the corresponding mRNA at 2 hr after injection of H2AA3-SL and H2AA3-SLD PCR products. C and N indicate cytoplasmic and nuclear FISH signals, respectively. C/N ratios were determined for 20 cells in each experiment. Data information: Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"H2AA3: 8337"
Figure legend: "(B) (Top) Illustration of the reporter constructs. The dashed line indicates the FISH probe. FISH to detect mRNA distribution at 24 hr after transfection of the H1C-SL-Rb (cH1C) and H1C-SLD-Rb (pH1C) constructs. C/N ratios were quantified Data information Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"H1C: 3006"
Figure legend: "(Top) Illustration of the reporter constructs. PCR products of cG-SL and cG-SLD were used instead. cG, β-globin cDNA. Data information: Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"β-globin: 3043"
Figure legend: "H1C-SL-Rb or H1C-SLD-Rb construct, together with VSV M were co-transfected into HeLa cells, followed by IPs with IgG or the ALYREF antibody at 24 hr post-transfection. The immunoprecipitates were subjected to western analysis to detect ALYREF (D)"
NCBI gene IDs:
"H1C: 3006"
Figure legend: "H1C-SL-Rb or H1C-SLD-Rb construct, together with VSV M were co-transfected into HeLa cells, followed by IPs with IgG or the ALYREF antibody at 24 hr post-transfection. RT-qPCRs to detect RNAs (E). The bars in the graph shows the relative ALYREF IP efficiency, with that for the H1C-SL-Rb mRNA set as &quot;1&quot;. Data information: Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"H1C: 3006"
Figure legend: "(F) H1C-SLD-Rb construct with wild-type (H1C-SLDWT-Rb) or mutant (H1C-SLDmut-Rb) U7-binding sequence, together with VSV M, was co-transfected into HeLa cells, followed by IPs with IgG or the ALYREF antibody at 24 hr post-transfection. The immunoprecipitates were subjected to western analysis (left panel) and RT-qPCRs (right panel). The bars in the right graph show the relative ALYREF IP efficiency, with that for the H1C-SLDWT-Rb mRNA set as &quot;1&quot;. Data information: Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. *P &lt; 0.05, ***P&lt; 0.001."
NCBI gene IDs:
"H1C: 3006"
Figure legend: "(A) Western blotting to examine the expression levels of H2B and H4 in Cntl, ALYREF and ARS2 KD cells. GAPDH was used as a loading control. The white line delineates the boundary where irrelevant lanes have been removed from the same blot"
NCBI gene IDs:
"ALYREF: 10189
ARS2: 51593"
Figure legend: "(B) Growth curve of Cntl and ALYREF KD cells. Error bars represent standard deviations from biological repeats (n=3). Statistical analysis was performed using Student's t-test. **P &lt; 0.01."
NCBI gene IDs:
"ALYREF: 10189"
Figure legend: "(C) Cell cycle analysis of Cntl, ALYREF and ARS2 KD cells. Quantifications of cells in G0/G1 (1N), G2 (2N) and S-phase (intermediate) of the cell cycle are shown in the upper right corner of each histogram."
NCBI gene IDs:
"ALYREF: 10189
ARS2: 51593"
Figure legend: "A. DIC images of abnormal embryo sacs in cycb1 mutant combinations. Red arrowheads indicate the visible nuclei in Col-0 embryo sacs (central and egg cells) and the corresponding structures in the quadruple cycb1;1-/- cycb1;2+/- cycb1;3+/- cyb1;4- mutant. Scale bars: 20 μm. B. Quantification of the different abnormal embryo sac structures in cycb1 mutant combinations (n = 202-459 embryo sacs per genotype). Different letters indicate significant differences in the proportion of abnormal embryo sacs in a Chi-squared test followed by the Marascuilo procedure to identify significant pairwise comparisons. WT = wildtype."
NCBI gene IDs:
"cycb1;2: 818444
cycb1;1: 829904
cycb1;3: 820325
cyb1;4: 817217"
Figure legend: "C. DIC images of embryo sacs 3 DAP with wildtype pollen (female x male). Red arrowheads indicate the visible embryo sac nuclei in the crosses with the quadruple cycb1;1-/- cycb1;2+/- cycb1;3+/- cyb1;4- mutant as a female donor, while the control Col-0 x Col-0 cross exhibits a developing embryo. Scale bars: 20 μm. D. Quantification of seed abortion in different cycb1 mutant combinations. Graph represents the average seed abortion rate per plant ± SD of three biological replicates, n = 463-579 seeds analyzed per genotype. Asterisks indicate significant differences in seed abortion rate in an ordinary one-way ANOVA test, followed by a Dunnett's multiple comparisons test (**** P &lt; 0.0001)."
NCBI gene IDs:
"cycb1;2: 818444
cycb1;1: 829904
cycb1;3: 820325
cyb1;4: 817217"
Figure legend: "A, B. DAPI staining of pollen in cycb1 mutants, including pollen configurations found in cycb1;1-/- cycb1;2+/- cycb1;3+/- cycb1;4-/- mutants (B). Scale bars: 5 μm. C. Quantification of DAPI-stained pollen configurations in different cycb1 mutant combinations, n = 420-616 pollen grains per genotype. Different letters indicate significant differences in the proportion of abnormal pollen (uni- and bicellular) in a Chi-squared test followed by the Marascuilo procedure to identify significant pairwise comparisons."
NCBI gene IDs:
"cycb1;2: 818444
cycb1;1: 829904
cycb1;3: 820325
cycb1;4: 817217"