EMBO/SourceData huggingface dataset:
Figure legend: "Alteration of retinal vascular maturation in TfebiEC-/- at p5. Representative images of whole mounts of retina and immunostaining of vascular front and vascular plexus with an anti-iB4 Ab (scale bars: 50 µm). Bar graphs indicate the percent of vascular area versus total area of the retina, the percent of vascular density, the number of branches per field, vessel diameter, the number of filopodia per field and the length of filopodia (mice n=10, mean±SEM; ***p&lt; 0.0001 versus control mice by Student's t-test)."
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Alteration of the glomerular ultrastructure in TfebiEC-/- mice at p17 (mice n=5). Representative transmission electron micrographs of renal tissue. Magnification: i) x6000, ii) x20000), iii-iv) x10000, v-vi) x25000; scale bars: 1µm). Asterisks indicate the accumulation of extracellular matrix in mesangium; white and black triangles indicate the fusion of podocyte foot processes and the lack of endothelial fenestrae, respectively."
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Reduced EC proliferation in the retina (p5) of in TfebiEC-/- mice. Representative images of vessels of the vascular front and vascular plexus in the retina of control and TfebiEC-/- mice stained with anti-iB4 and Ki-67 antibodies (scale bars: 50 µm). Bar graph indicates the percentage of EC Ki-67+ nuclei versus total nuclei co-localized with iB4+ vessels"
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Reduced EC proliferation in the kidney (p17) of in TfebiEC-/- mice. (B) Representative images of vessels of the kidney in control and TfebiEC-/- mice stained with anti-CD31 and Ki-67 antibodies (scale bars: 50 µm). Bar graph indicates the percent of EC Ki-67+ nuclei versus total nuclei co-localized with CD31+ vessels (mice n=6, mean±SEM; **p&lt;0.001 versus control mice by Student's t-test). Podocin staining is shown to glomerular localization."
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "TFEB silencing reduced EC proliferation. Representative graph of scr-shRNA and sh-TFEB ECs treated for 24 hours with FCS (20%) or VEGF-A (30 ng/ml). (C) DNA incorporation of EdU was detected by flow cytometry. The percentage of proliferating cells is indicated (n=4, mean±SEM; ***p&lt;0.0001 versus scr-shRNA ECs by Student's t-test)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "TFEB silencing reduced EC proliferation. Representative graph of scr-shRNA and sh-TFEB ECs treated for 24 hours with FCS (20%) or VEGF-A (30 ng/ml). DNA content was determined by propidium iodide staining and assessed by fluorescences-activated cell sorter analysis (representative experiment out of 4 with similar results)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "TFEB silencing reduced human EC morphogenesis. Representative images of tube-like-structure of scr-shRNA and sh-TFEB human ECs stained with phalloidin-555. Bar graph indicates the percentage of phalloidin+ area in sh-TFEB and scr-shRNA ECs (scale bars: 0.25 mm; n=6, mean±SEM; ***p&lt;0.0001 versus scr-shRNA by Student's t-test)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Heatmap showing unsupervised hierarchical clustering of human differentially expressed genes between human scr-shRNA and sh-TFEB ECs. Red: up-regulated genes; green: down-regulated genes."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "qPCR Data are expressed as relative fold-change compared with the expression in scr-shRNA cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: "
Figure legend: "immunoblots showing the differentially expressed cell cycle related genes between scr-shRNA and sh-TFEB ECs. Immunoblots of total lysates from scr-shRNA and sh-TFEB ECs probed with specific Abs. The bar graph shows the densitometric analysis expressed as the ratio between the cell cycle genes and α-tubulin"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Modulation of cell cycle genes expression in the lung ECs derived from control and TfebiEC-/- mice. Data are expressed as relative fold-change compared with the expression in ECs of control mice after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
Tfeb: 21425"
Figure legend: "Analysis of TFEB binding and modulation of the CDK4 promoter in human ECs. ChIP was performed using digested chromatin from control ECs and TFEBS142A ECs incubated with IgG (indicated in the bar graph as &quot;+IgG&quot;) or with Ab anti-TFEB (indicated in the bar graph as &quot;+Ab anti-TFEB&quot;), followed by qPCR for CDK4. Bar graph shows the percent enrichment (n=3, mean±SD)."
NCBI gene IDs:
"CDK4: 1019
TFEB: 7942"
Figure legend: "Analysis of TFEB binding and modulation of the CDK4 promoter in human ECs. Analysis of TFEB modulation of CDK4 promoter in human ECs. Bar graph shows the relative luciferase activity % evaluated in control and TFEBS142A human ECs after transfection of CDK4 full promoter and CDK4 promoter deleted of TFEB binding site"
NCBI gene IDs:
"CDK4: 1019
TFEB: 7942"
Figure legend: "Modulation of Rb protein in sh-TFEB ECs. Immunoblots of total lysates from scr-shRNA and sh-TFEB ECs probed with phospho-Rb and total Rb Abs. The bar graph shows the densitometric analysis of the immunoblotting expressed as % of sh-TFEB vs scr-shRNA and the ratio between phospho-Rb and total Rb in the different conditions"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Increase of Vegfr2 expression in the vasculature of TfebEC-/- mice. Representative immunostaining images of TfebEC-/- embryonic vessels (E10.5) stained with anti-endomucin and anti-Vegfr2 Abs (scale bars: 50 µm). Bar graph indicates the Vegfr2 mean intensity only in endomucin+ vessel areas (embryos n=6, mean±SEM; ***p&lt; 0.0001 versus control embryos by Student's t-test)."
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Increase of Vegfr2 expression in the vasculature of TfebiEC-/- mice. Representative immunostaining images (i) and detail (ii) of the vascular front and vascular plexus of the retina (p5) of control and TfebiEC-/- mice with anti-iB4 and anti-Vegfr2 Abs (scale bars: 50 µm). Bar graph indicates the Vegfr2 mean intensity only in iB4+ vessel areas"
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Increase of Vegfr2 expression in the vasculature of TfebiEC-/- mice. Representative immunostaining images of the glomerulus (p17) of control and TfebiEC-/- mice with anti-podocin, anti-CD31 and anti-Vegfr2 Abs (scale bars: 50 µm). Bar graphs indicate the Vegfr2 mean intensity in CD31+ vessel areas"
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "VEGFR2 expression is regulated by TFEB. qPCR of VEGFR2 in lung ECs obtained from control and TfebiEC-/- mice (D) Data are expressed as relative fold-change compared with the expression in control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
VEGFR2: 16542
TFEB: 21425
Tfeb: 21425"
Figure legend: "VEGFR2 expression is regulated by TFEB. qPCR of VEGFR2 in lung ECs obtained from control and in human sh-TFEB Data are expressed as relative fold-change compared with the expression in control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
VEGFR2: 3791
TFEB: 7942"
Figure legend: "VEGFR2 expression is regulated by TFEB. Immunoblots of total lysates from scr-shRNA and sh-TFEB ECs probed with anti-VEGFR2 and α-tubulin Abs. The bar graph shows the densitometric analysis expressed as the ratio between VEGFR2 and α-tubulin"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Analysis of TFEB binding to the VEGFR2 promoter in human ECs. ChIP was performed using digested chromatin from human control ECs and TFEBS142A ECs incubated with IgG (indicated in the bar graph as &quot;+IgG&quot;) or with Ab anti-TFEB (indicated in the bar graph as &quot;+Ab anti-TFEB&quot;), followed by qPCR for VEGFR2."
NCBI gene IDs:
"VEGFR2: 3791
TFEB: 7942"
Figure legend: "Silenced TFEB alters the localization of VEGFR2. FACS analysis of surface VEGFR2 expression on human scr-shRNA and sh-TFEB ECs. Bar graph shows the ratio between total and PM VEGFR2"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Silenced TFEB alters the localization of VEGFR2. Representative TIRF and epifluorescence images of human scr-shRNA and sh-TFEB ECs after staining with anti-VEGFR2 Ab (scale bars: 10 µm). Bar graphs show the ratio of VEGFR2 analyzed in epifluorescence and TIRF mode analyzed by TIRF"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Silenced TFEB alters the localization and the phosphorylation state of VEGFR2 and its signal transduction. Representative immunoblot of PM biotinylated portion and total cell lysates of scr-shRNA and sh-TFEB ECs after VEGF-A stimulation (30 ng/ml). Blots of total or PM cell lysates were probed with anti-VEGFR2. Blots of total cell lysates were probed with anti-p-Y1175-VEGFR2, anti-PLCγ, p-PLCγ, anti-ERK-1/2, anti-pERK1/2, anti-p-Src, anti-Src, anti CD31 and α-tubulin Abs. The bar graphs (i,ii) show densitometric analysis of stimulated versus unstimulated scr-shRNA and sh-TFEB ECs expressed as: (i) % of VEGFR2 on PM fraction (n=3, mean±SEM; ANOVA p&lt;0.02; **p&lt;0.001 versus scr-shRNA by Bonferroni post-test), (ii) % of VEGFR2 total"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Regulaton of autophagy and lysosome pathway by TFEB silencing. qPCR showing the differentially expressed autophagy and lysosome related genes between human scr-shRNA and sh-TFEB ECs. Data are expressed as relative fold-change compared with the expression in control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
TFEB: 7942"
Figure legend: "Regulaton of autophagy and lysosome pathway by TFEB silencing. immunoblots showing the differentially expressed autophagy and lysosome related genes between human scr-shRNA and sh-TFEB ECs. Immunoblots of total lysates from human scr-shRNA and sh-TFEB ECs probed with anti-ULK-1, anti-ATG9, anti-LC3-I/II, anti-LAMP-1 and α-tubulin Abs. The bar graph shows the densitometric analysis expressed as the ratio between scr-shRNA and sh-TFEB ECs"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "TFEB silencing inhibits VEGFR2 internalization. Bar graphs of VEGFR2 internalization expressed as the percent of internalized VEGFR2 versus PM VEGFR2 after VEGF-A stimulation"
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "Analysis of TFEB binding and modulation of the MYO1C promoter in human ECs. ChIP was performed using digested chromatin from control ECs and TFEBS142A ECs incubated with IgG (indicated in the bar graph as &quot;+IgG&quot;) or with Ab anti-TFEB (indicated in the bar graph as &quot;+Ab anti-TFEB&quot;), followed by qPCR for MYO1C. Bar graph shows the percent enrichment (n=3, mean±SD)."
NCBI gene IDs:
"MYO1C: 4641
TFEB: 7942"
Figure legend: "Analysis of TFEB binding and modulation of the MYO1C promoter in human ECs. qPCR of MYO1C expression in scr-shRNA, sh-TFEB, sh-MYO1C and sh-TFEB+sh-MYO1C ECs. Data are expressed as relative fold change compared with the expression in scr-shRNA ECs after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
MYO1C: 4641
TFEB: 7942"
Figure legend: "MYO1C silencing reverses the effect of TFEB silencing on the up-regulation of PM VEGFR2. Analyses were performed on human ECs carrying appropriate scr-shRNA or sh-TFEB in the presence or absence of sh-MYO1C. Representative western blot of MYOC1, total and PM biotinylated VEGFR2 (representative experiment out of 4 with similar results)."
NCBI gene IDs:
"MYO1C: 4641
TFEB: 7942"
Figure legend: "Representative immunostaining images of the vascular plexus of the retina (p5) (E) and glomerulus (p17)(F) of control and TfebiEC-/- mice with anti-CD31 and anti-MYO1C Abs (scale bars: 50 µm). Bar graphs indicate the Myo1C mean intensity only in vessel areas CD31+"
NCBI gene IDs:
"Tfeb: 21425"
Figure legend: "Representative TIRF and epifluorescence images of VEGFR2 in scr-shRNA, sh-TFEB, sh-MYO1C and sh-TFEB+sh-MYO1C human ECs (scale bars: 10 µm). Bar graph shows the ratio between PM and total VEGFR2"
NCBI gene IDs:
"MYO1C: 4641
TFEB: 7942"
Figure legend: "Analysis of TFEB binding to DLEU2 and SMC4 promoters in human ECs. ChIP was performed using digested chromatin from control ECs and TFEBS142A ECs incubated with IgG (indicated in the bar graph as &quot;+IgG&quot;) or with Ab anti-TFEB (indicated in the bar graph as &quot;+Ab anti-TFEB&quot;), followed by qPCR for DLEU2 and SMC4."
NCBI gene IDs:
"DLEU2: 8847
SMC4: 10051
TFEB: 7942"
Figure legend: "DLEU2 expression is regulated by TFEB. qPCR of DLEU2 in human scr-shRNA, sh-TFEB or control and TFEBS142A ECs (left panel) and lung ECs obtained from control and TfebiEC-/- mice (right panel). Data are expressed as relative fold-change compared with the expression in scr-shRNA and control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
DLEU2: 8847
TFEB: 7942
Tfeb: 21425"
Figure legend: "Human miR-15a-5p and miR-16-5p are regulated by TFEB. qPCR of miR-15a-5p (left panel) and miR-16-5p (right panel) in sh-TFEB or TFEBS142A ECs. Data are expressed as relative fold-change compared with the expression in scr-shRNA and control cells after normalization to the housekeeping gene RNU44"
NCBI gene IDs:
"RNU44: 
miR-15a-5p: 8847
miR-16-5p: 406950
TFEB: 7942"
Figure legend: "VEGFR2 expression is regulated by TFEB through a miR-dependent mechanism. qPCR of VEGFR2 in human scr-shRNA and sh-TFEB ECs and in control treated with a specific miR-control, miR-15a-5p and miR-16-5p mimics Data are expressed as relative fold-change compared with the expression in control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
miR-15a-5p: 8847
VEGFR2: 3791
miR-16-5p: 406950
TFEB: 7942"
Figure legend: "VEGFR2 expression is regulated by TFEB through a miR-dependent mechanism. VEGFR2 in human scr-shRNA and sh-TFEB ECs and in control treated with a specific miR-control, miR-15a-5p and miR-16-5p mimics Representative western blot of VEGFR2 expression under the same experimental conditions previously reported. The bar graph shows the densitometric analysis expressed as the ratio between VEGFR2 and α-tubulin"
NCBI gene IDs:
"miR-15a-5p: 8847
miR-16-5p: 406950
TFEB: 7942"
Figure legend: "VEGFR2 expression is regulated by TFEB through a miR-dependent mechanism. qPCR of VEGFR2 in control and TFEBS142A ECs treated with a specific miR-control, miR-15a-5p and miR-16-5p inhibitors. Data are expressed as relative fold-change compared with the expression in control cells after normalization to the housekeeping gene TBP"
NCBI gene IDs:
"TBP: 
miR-15a-5p: 8847
VEGFR2: 3791
miR-16-5p: 406950
TFEB: 7942"
Figure legend: "VEGFR2 expression is regulated by TFEB through a miR-dependent mechanism. VEGFR2 in control and TFEBS142A ECs treated with a specific miR-control, miR-15a-5p and miR-16-5p inhibitors. Representative western blot of VEGFR2 expression under the same experimental conditions previously reported. The bar graph shows the densitometric analysis expressed as the ratio between VEGFR2 and α-tubulin"
NCBI gene IDs:
"miR-15a-5p: 8847
miR-16-5p: 406950
TFEB: 7942"
Figure legend: "B-D. Mice were analyzed by Kaplan-Meier survival plot (B), the percentage of body weight (C), and the clinical scores (D) of WT and GPx8-/- mice with colitis induced by 4% DSS for 6 days (n = 10 per group). Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "Gut microbiome was shaped by Gpx8 deficiency under standard housing conditions. Bray Curtis dissimilarity of fecal microbiota were measured by bacterial 16S RNA sequencing (n = 5 per group) and are presented by a heat map, (I), and principal coordinates analysis, (J)."
NCBI gene IDs:
"Gpx8: 69590"
Figure legend: "Gut microbiome was shaped by Gpx8 deficiency under standard housing conditions. (K) α-diversity indicated bacteria species in each mouse depicted by richness (total operational taxonomic units, OTUs), the Chao1 richness estimator (Chao1), and the abundance-based coverage estimator (ACE) metrics. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"Gpx8: 69590"
Figure legend: "After undergoing macrophage depletion, C57BL/6 mice received macrophages derived from WT (WT BMDMs) or GPx8-/- mice (GPx8-/- BMDMs) were under DSS-induced colitis (n = 6-9 per group). (C) The percentage of body weight. (D) The clinical scores of mice. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's one‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "After undergoing macrophage depletion, C57BL/6 mice received macrophages derived from WT (WT BMDMs) or GPx8-/- mice (GPx8-/- BMDMs) were under DSS-induced colitis (n = 6-9 per group). Colon samples from BMDMs transplanted mice were collected on day 7 under colitis model. (E) Statistical analysis of colon length. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's one‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "After undergoing macrophage depletion, C57BL/6 mice received macrophages derived from WT (WT BMDMs) or GPx8-/- mice (GPx8-/- BMDMs) were under DSS-induced colitis (n = 6-9 per group). Colon samples from BMDMs transplanted mice were collected on day 7 under colitis model. (F) Images and semiquantitative scoring of hematoxylin and eosin staining colon sections. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's one‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "After undergoing macrophage depletion, C57BL/6 mice received macrophages derived from WT (WT BMDMs) or GPx8-/- mice (GPx8-/- BMDMs) were under DSS-induced colitis (n = 6-9 per group). Colon samples from BMDMs transplanted mice were collected on day 7 under colitis model. (G) Colon sections stained with anti-F4/80. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's one‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "After undergoing macrophage depletion, C57BL/6 mice received macrophages derived from WT (WT BMDMs) or GPx8-/- mice (GPx8-/- BMDMs) were under DSS-induced colitis (n = 6-9 per group). D) Colon samples from BMDMs transplanted mice were collected on day 7 under colitis model. (H) Production of IL-1β, IL-6 and IL-18 in colon tissue lysates. Data information: Data are presented as the mean ± SEM. *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's one‐tailed t‐test). "
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "A. Canonical NLRP3 inflammasome activation was not altered by GPx8 deficiency. BMDMs were primed for 6 h with 0.5 μg/ml LPS and then transfected with LPS (TF LPS) for 8 h, stimulated with ATP (5 mM) for 3 h, MSU (150 μg/mL) for 8 h, or nigericin (5 μM) for 3 h. Activation of inflammasome was demonstrated by secreted IL-1β. Data information: data are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats. P > 0.05; *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "B. GPx8 deficiency enhanced noncanonical inflammasome activation. Activation of the noncanonical inflammasome by different amounts of cytoplasmic LPS was verified by IL-1β (left panel) and LDH release (right panel). Data information: data are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats. P &gt; 0.05; *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "C. Upregulation of noncanonical inflammasome activation by GPx8 deficiency occurred independently of TLRs. BMDMs were primed for 6 h with 1 μg/ml poly(I:C) and then transfected with LPS for 8 h. Data information: data are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats. P > 0.05; *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "Over-activation of noncanonical inflammasome induced by GPx8 deficiency in BMDMs can be rescued by ectopically-expressing human GPx8 (hGPx8). (D) Activation of noncanonical inflammasome in BMDMs with empty vector (EV) or hGPx8 was verified by IL-1β (left panel) and LDH release (right panel). Data information: data are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats. P > 0.05; *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590
GPx8: 493869
hGPx8: 493869"
Figure legend: "Over-activation of noncanonical inflammasome induced by GPx8 deficiency in BMDMs can be rescued by ectopically-expressing human GPx8 (hGPx8). (E) Expression of hGPx8 mRNA in BMDMs was confirmed by quantitative reverse transcriptase PCR. Data information: data are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats."
NCBI gene IDs:
"GPx8: 69590
GPx8: 493869
hGPx8: 493869"
Figure legend: "F. GPx8 deficiency enhanced caspase-11 cleavage and activation. Levels of processed caspase-1 and -11 in the supernatants (Sup) and full-length caspase-1, -11 and GAPDH in the cell lysates (Cell ext) were determined by immunoblotting. Fold changes of protein expression were normalized with internal controls and are indicated below the blots."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "G. RNA expression of caspase-11 was not altered by GPx8 deficiency. Caspase-11 RNA expression of BMDMs isolated from GPx8-/- mice was measured after LPS priming. Data information: ata are representative of at least 3 independent experiments and presented as the mean ± SD, n=4-5, technical repeats."
NCBI gene IDs:
"caspase-11: 12363
Caspase-11: 12363
GPx8: 69590"
Figure legend: "H. GPx8 deficiency enhanced noncanonical inflammasome activation and pyroptosis in vivo. Colon samples from 5 WT or GPx8-/- mice treated with DSS for 5 days were collected on day 7. Levels of full-length and processed caspase-1, -11 and gasdermin D (GSDMD) in tissue lysates were individually analyzed by immunoblots."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "GPx8-/- mice were more susceptible than WT mice to endotoxic shock by activation of the noncanonical inflammasome. (I) Survival of mice primed with ultra pure LPS (E. coli O55:B5, 400 μg/kg) and re-challenged 6 h later with LPS (40 mg/kg) (n = 8 per group)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "GPx8-/- mice were more susceptible than WT mice to endotoxic shock by activation of the noncanonical inflammasome. (J) Survival of mice primed with poly(I:C) (LMW 1 mg/kg) and re-challenged 6 h later with LPS (10 mg/kg) (n = 9 per group)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "GPx8-/- mice were more susceptible than WT mice to endotoxic shock by activation of the noncanonical inflammasome. (K) IL-1β production levels in mice sera were measured 2 h after being primed with poly(I:C) (LMW 1 mg/kg) and re-challenged 6 h later with LPS (1 mg/kg) (n = 8-9 per group). In (K), data are presented as the mean ± SEM. NS, no significance. P &gt; 0.05; *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001 (Student's two‐tailed t‐test)."
NCBI gene IDs:
"GPx8: 69590"
Figure legend: "A. Interaction of GPx8 with human caspase-4 (Casp4) or mouse caspase-11 (Casp11) was confirmed by co-IP assays. 293T cells were co-transfected with vectors expressing FLAG tagged Casp4 or Casp11, in addition to GPx8 expressing vectors. Cell lysate containing Casp4- or Casp11-FLAG proteins was immunoprecipitated by anti-FLAG Ab and subsequently analyzed by Western blots using anti-GPx8 and anti-FLAG Ab (left panel). GPx8 and its interacting proteins were precipitated by anti-GPx8 Ab and analyzed by the indicated Abs (right panel)."
NCBI gene IDs:
"FLAG: 
GPx8: 
Casp11: 12363
Casp4: 837"