EMBO/SourceData huggingface dataset:
Figure legend: "H αSMA mRNA expression in TGFβ-treated HFL1 cells overexpressing two RNase dead mutants (K599A and K907A) of IRE1α. *P K599A = 0.013, *P K907A = 0.0008 compared to WT."
NCBI gene IDs:
"αSMA: 59
IRE1α: 2081"
Figure legend: "I αSMA mRNA expression in TGFβ-treated IRE1α -/- or WT MEF cells. *P = 0.025."
NCBI gene IDs:
"αSMA: 11475
IRE1α: 78943"
Figure legend: "A miR-150 levels in TGFβ-treated HFL1 fibroblasts and the effect of the IRE1α inhibitor 4μ8C. * P = 0.046 compared to control."
NCBI gene IDs:
"miR-150: 406942"
Figure legend: "B miR-150 levels in HFL1 fibroblasts overexpressing two RNase dead mutants (K599A and K907A) of IRE1α. * P = 0.0043 (K599A), P = 0.043 (K907A), compared to control."
NCBI gene IDs:
"IRE1α: 2081
miR-150: 406942"
Figure legend: "C miR-150 levels in TGFβ-treated WT (IRE1α +/+) and IRE1α -/- MEF cells. *P = 0.018. Results are expressed as fold change compared to the Ctrl."
NCBI gene IDs:
"IRE1α: 78943
miR-150: 387168"
Figure legend: "D miR-150 levels in IRE1α -/- MEF cells that were either untransfected (UT) or transfected with miRNA inhibitor control (siCtrl) or with a miR-150-5p miRNA inhibitor. * P = 0.034 compared to UT."
NCBI gene IDs:
"IRE1α: 78943
miR-150: 387168"
Figure legend: "E αSMA mRNA expression in TGFβ-treated IRE1α -/- MEF cells that were either untransfected (UT) or transfected with miRNA inhibitor control (siCtrl) or with a miR150-5p miRNA inhibitor. *P = 0.043 compared to UT."
NCBI gene IDs:
"αSMA: 11475
IRE1α: 78943
miR150: 387168"
Figure legend: "F miR-150 levels in HFL1 fibroblasts transfected with a control plasmid (Ctrl) or a miR-150 expression plasmid (miR150). * P = 0.038."
NCBI gene IDs:
"miR-150: 406942
miR150: 406942"
Figure legend: "G Western blot of c-Myb in TGFβ-treated HFL1 fibroblasts that were either untransfected (UT), transfected with a control plasmid (pCtrl) or a miR150 expression plasmid (miR150). *P = 0.0206 in UT Ctrl vs. UT TGFβ and P = 0.0126 in pCtrl Ctrl vs. pCtrl TGFβ ."
NCBI gene IDs:
"miR150: 406942"
Figure legend: "I c-Myb mRNA levels in untransfected HFL1 cells (UT) and in cells transfected with a control siRNA (siCtrl) or an siRNA targeting c-Myb (sicMyb). * P = 0.00001 compared to UT."
NCBI gene IDs:
"c-Myb: 4602"
Figure legend: "J αSMA mRNA expression in untransfected HFL1 cells (UT) and in cells transfected with a control siRNA (siCtrl) or with a siRNA targeting c-Myb (sicMyb). * P = 0.0042 in UT Ctrl vs. UT TGFβ."
NCBI gene IDs:
"αSMA: 59
c-Myb: 4602"
Figure legend: "L In vitro cleavage assay with recombinant IRE1α and miR-150 (left) or XBP-1 (right)."
NCBI gene IDs:
"miR-150: 406942"
Figure legend: "M miR-150 expression in HFL-1 cells that were pre-treated with TGFβ in the absence of presence of 4μ8C and then treated with Actinomycin D (ActD) for 4 and 24 hours. * P = 0.027 compared to TGFβ."
NCBI gene IDs:
"miR-150: 406942"
Figure legend: "A Analysis of ER size in HFL1 fibroblasts treated with TGFβ in the absence (Ctrl) or presence of the IRE1α inhibitor 4μ8C, or in HFL1 cells transfected with a siRNA control siRNA (siCtrl) or with siRNA targeting XBP-1 (siXBP-1). The ER was visualized with ER tracker Red and nuclei stained with Hoechst (left, scale bars = 20 μm) or by electron microscopy (right, scale bars = 200 nm) where red lines mark the ER.B Quantification of ER size relative to the nucleus from the experimental setup in A. * P = 0.0001 in Ctrl, and * P = 0.0223 in siCtrl."
NCBI gene IDs:
"XBP-1: 7494"
Figure legend: "C XBP-1 mRNA expression in untransfected HFL1 cells (UT) and in cells transfected with control siRNA (siCtrl) or siRNA targeting XBP-1 (siXBP-1). * P = 0,0001."
NCBI gene IDs:
"XBP-1: 7494"
Figure legend: "E BiP mRNA expression in liver tissue from mice presented in A. * P = 0,020."
NCBI gene IDs:
"BiP: 14828"
Figure legend: "F Representative gel showing levels of spliced and unspliced XBP-1 in liver tissue from control and CCl4-induced cirrhotic mice and the effect of 4μ8C treatment."
NCBI gene IDs:
"XBP-1: 22433"
Figure legend: "G miR150 expression in liver tissue from mice in A. * P = 0,032 compared to untreated controls."
NCBI gene IDs:
"miR150: 387168"
Figure legend: "H αSMA mRNA levels in primary stellate cells isolated from mouse liver and then treated or not with TGFβ ± 4μ8C. * P = 0,031, n = 3."
NCBI gene IDs:
"αSMA: 11475"
Figure legend: "I BiP mRNA levels in primary stellate cells isolated from mouse liver and then treated with or without TGFβ ± 4μ8C. * P = 0,008, n = 3."
NCBI gene IDs:
"BiP: 14828"
Figure legend: "L Quantification of mRNA expression levels of CTGF and collagen in primary human hepatic stellate cells stimulated with TGFβ ±4μ8C."
NCBI gene IDs:
"CTGF: 1490"
Figure legend: "D Representative image of the area selected for laser capture microdissection (scale bars = 100 μm).E Expression levels of αSMA mRNA in samples of hypodermis obtained by laser capture microdissection. Ctrl (n = 5), Ctrl + 4μ8C (n = 5), TGFβ (n = 5), TGFβ + 4μ8C (n = 5). * P = 0,044.F miR-150 expression levels in the same samples as shown in E."
NCBI gene IDs:
"αSMA: 11475
miR-150: 387168"
Figure legend: "H αSMA mRNA expression in primary mouse skin fibroblasts after treatment with TGFβ ± 4μ8C. * P = 0,049, n = 4."
NCBI gene IDs:
"αSMA: 11475"
Figure legend: "I Agarose gel showing levels of spliced and unspliced XBP-1 in primary mouse skin fibroblasts treated as indicated with TGFβ and 4μ8C."
NCBI gene IDs:
"XBP-1: 22433"
Figure legend: "J BiP mRNA expression in primary mouse skin fibroblasts after treatment with TGFβ in the absence and presence of 4μ8C. * P = 0,00007 for TGFβ vs. Ctrl and P = 0,00002 for TGFβ vs. TGFβ + 4μ8C. n=4."
NCBI gene IDs:
"BiP: 14828"
Figure legend: "L miR-150 mRNA levels in primary mouse skin fibroblasts treated as indicated with TGFβ and 4μ8C. * P = 0,00031 compared to untreated control, n = 3."
NCBI gene IDs:
"miR-150: 387168"
Figure legend: "A CTGF mRNA expression levels in skin (dSSc) and lung (LSSc) fibroblasts isolated from scleroderma patients and treated with the IRE1α inhibitor 4μ8C. * P = 0,018 for dSSc (n = 6) and P = 0,003 for LSSc (n = 3)."
NCBI gene IDs:
"CTGF: 1490"
Figure legend: "B Collagen1α2 mRNA expression levels in the same samples as in A. * P = 0,0030 for dSSC and P = 0,0036 for LSSc."
NCBI gene IDs:
"Collagen1α2: 1278"
Figure legend: "E mRNA levels of αSMA in primary lung fibroblasts isolated from patients with either cystic fibrosis (CF), chronic obstructive pulmonary disease (COPD) or asthma. * P = 0,00001 for asthma vs. healthy controls, and P = 0,00003 for ctrl vs. 4μ8C in asthma patients."
NCBI gene IDs:
"αSMA: 59"
Figure legend: "F Quantification of mRNA levels for BiP in same samples as in E. * P = 0,0023 between asthma and healthy controls and P = 0,0031 between 4μ8C-treated and control treatment in fibroblasts from asthma patients."
NCBI gene IDs:
"BiP: 3309"
Figure legend: "(B) Flow cytometry  analysis of WT to Il21r-/- splenic  SWHEL B cells over time. (C) WT to Il21r-/- SWHEL B cell ratio within CTV  division peaks. Data information: Data in (B) is representative of 8-9 biological replicates (n = 8-9) pooled from two independent experiments with statistical analysis by one-way ANOVA with Tukey's post-test. ** p ≤ 0.01; **** p ≤ 0.0001. Data show concatenated data from 4-5 biological replicates (n = 4-5) and are representative for two independent experiments."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "(B) Flow cytometry  analysis showing WT to Il21r-/- SWHEL B cell  ratio of cells that have been in S phase and thus incorporated BrdU. Data information: Data are representative of 5-7 biological replicates (n = 5-7) from two independent experiments. Statistical analysis by one-sample t test. *p ≤ 0.05."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "(D) Ratio of the proportion of BrdU  positive WT and Il21r-/- SWHEL B cells with 2N DNA  content 4, 10 and 12 hours post BrdU  pulse on day 3.5 post immunization. Data information: Data are representative of 5-7 biological replicates (n = 5-7) from two independent experiments. Statistical analysis by one-sample t test. *p ≤ 0.05."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "(C) Rate of de novo  cell cycle entry 10 hours post in vivo IL-21 (2 µg) or saline  pulse. The ratio of WT to Il21r-/- cells not in S/G2 at the time of pulse (BrdU- cells) and containing &gt;2N DNA  content 10 hours after pulse is shown. Data information: Data are representative of 10-12 biological replicates (n = 10-12) from two independent experiments. Statistical analysis by one-sample t test. * p ≤ 0.05."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "Phosphoflow analysis  of naïve WT or Il21r-/- B cells following in vitro culture for 3 hours with or without IL-21 (20 ng/mL) and/or in the presence of BCR  cross-linking (biotinylated anti-Igκ  + anti-Igλ  and avidin-mediated cross-linking) or agonistic anti-CD40. (A) Exemplary p-AKT  (S473) staining  and (B) quantification of p-AKT  median fluorescence intensity (MedFI). (C) Exemplary p-S6  (Ser235/236) staining  and (D) quantification of frequency of p-S6  positive cells Data information: Data are representative of 4 biological replicates (n = 4). Statistical analysis by one-way ANOVA with Tukey's post-test (D), t test (B, E) * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "(B, C) Analysis of splenic  OTII  Tfh  cells by flow cytometry. (B) Tfh cell count  and (C) Proportion of Il21Gfp/+ OTII Tfh   cells transcribing the Il21  locus and thus expressing GFP. Data information: Data are representative of 5-6 biological replicates (n = 5-6) form two independent experiments. Statistical analysis by one-way ANOVA with Tukey's post-test (B, C). * p ≤ 0.05; ** p ≤ 0.01."
NCBI gene IDs:
"GFP: 
Il21: 60505"
Figure legend: "Analysis of splenic  WT and Il21r-/- SWHEL B cells  by  flow cytometry. (D) CTV  profile of and (E) absolute  cell count in pooled CTV  division peaks (0-4, 5-8, 9-11+). Data information: Data are representative of 5-6 biological replicates (n = 5-6) form two independent experiments. D, show concatenated data representative of 3-5 biological replicates. Data in E were analyzed after log transformation using Multiple paired t tests and p values corrected for multiple comparisons using Holm-Šídák method. * p ≤ 0.05; ** p ≤ 0.01."
NCBI gene IDs:
"Il21r: 60504"
Figure legend: "(A-E) Analysis of splenic  SWHEL B cells and PC  by  flow cytometry. (A) Proportion of SWHEL B cells with a FAS+GL7+ GC  phenotype, (B) SWHEL GC B cell count and (C) representation of  IL-21R deficient cells among total SWHEL GC B cells. (D) CD98+ CD138+_SWHEL PC count and (E) representation of IL-21R  deficient cells among total SWHEL PC. Data information: Data are representative of 6-10 biological replicates (n = 6-10) from two independent experiments. Data in B, D were analyzed after log transformation with with statistical analysis by Multiple paired t tests (A, B, D) and p-values corrected for multiple comparisons using Holm-Šídák method or One-way ANOVA with Tukey's post-test (C) or t test (E)."
NCBI gene IDs:
"IL-21R : 60504
IL-21R: 60504"
Figure legend: "WB SecA with additional mutations (WT, Y794G, R342E) was added to preformed intermediates (IM) and incubated for the indicated times before proteolysis. The gel is representative of two replicates.   Quantification of (B). Points show IM band intensities normalized to the 0-min time point for each replicate. Lines show average of replicates. Colors correspond to WB SecA carrying additional mutations, as shown in the inset box.   "
NCBI gene IDs:
"SecA: 944821"
Figure legend: "Imidazole (imi) and either WT or mutant SecA were added to translocation intermediates (IM) pre-formed with SecA dN20::H6. The samples were incubated for the indicated times before proteolysis with proteinase K (Prot. K). The gel is representative of two replicates, except for the experiment with the R792G/K797G mutant and the 30-min time points. Quantification of (B). Points show IM band intensities normalized to the 0-min time point for each replicate. Lines show average of replicates. Colors correspond to different SecA mutants as shown in the inset box. As in (B), but ATPγS was added together with imidazole. The gel is representative of two replicates. Quantification of (E) as in (C). As in (D), but DTT was added with ATPγS ten minutes after the addition of imidazole and WT or mutant SecA. The gel is representative of two replicates.   Quantification of (G) as in (F), except that both the intensities of the IM (darker colors) and FL (lighter colors) bands were measured.   "
NCBI gene IDs:
"SecA: 944821"
Figure legend: "A. Arginine methylase inhibitor decreases SIRT7 methylation. HEK293T cells expressing Flag-tagged SIRT7 were treated with increasing doses of AdOx as indicated for 24 hrs. After immunoprecipitated with Flag-beads, SIRT7 was analyzed by western blot and detected by an antibody against mono-methylarginine (α-me1). Relative methylation ratio (Ratio) was calculated by normalizing arginine methylation level against the Flag-SIRT7 protein. WCL, whole cell lysate"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "C. PRMT inhibitor reduces arginine methylation of wildtype SIRT7, but not its RK/RF mutants. HEK293T cells expressing wildtype SIRT7 or its RK/RF mutants were treated with or without AdOx for 24 hrs. Arginine methylation of immunopurified SIRT7 was determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "D. SIRT7 is mono- and asymmetrically di-methylated at R388. Flag-tagged SIRT7 and its RK/RF mutants were expressed in HEK293T cells. Immunopurified SIRT7 was blotted with antibodies against mono-methylarginine (α-me1), asymmetric di-methylarginine (α-me2a), and symmetric di-methylarginine (α-me2s), respectively"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "H. SIRT7 is methylated at R388. HEK293T cells ectopically expressing Flag-tagged SIRT7 were treated with increasing concentrations of AdOx for 24 hrs as indicated. SIRT7 protein was pulled down by Flag-beads. Arginine methylation of SIRT7 was detected with a site-specific antibody against R388 methylation [α-meSIRT7(R388)]"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "B. Co-expression of PRMT6, but not PRMT1 or PRMT3, increases R388 methylation of SIRT7. Flag-tagged SIRT7 and GFP-tagged PRMT1, PRMT3, or PRMT6 were co-expressed in HEK293T cells. R388 methylation of immunopurified SIRT7 was analyzed by western blot"
NCBI gene IDs:
"PRMT3: 
PRMT1: 3276
PRMT6: 55170
SIRT7: 51547"
Figure legend: "C. Wildtype PRMT6, but not its catalytic-deficient mutant, upregulates SIRT7 R388 methylation. Wildtype PRMT6 or its V86K/D88A mutant (DM) was co-expressed with Flag-tagged SIRT7 in HEK293T cells. R388 methylation of immunopurified SIRT7 was detected by western blot"
NCBI gene IDs:
"PRMT6: 55170
SIRT7: 51547"
Figure legend: "D-E. PRMT6 physically interacts with endogenous SIRT7. Endogenous SIRT7 (D) or PRMT6 (E) was pulled down using corresponding antibody from HEK293T, MEF, and primary mouse hepatocytes, respectively. Input and immunoprecipitates were analyzed by western blot"
NCBI gene IDs:
"PRMT6: 55170
PRMT6: 99890
SIRT7: 51547
SIRT7: 209011"
Figure legend: "G. PRMT6 inhibitor reduces arginine methylation of wildtype SIRT7, but not RK/RF mutants. Wildtype SIRT7 or its RK/RF mutants were stably expressed in MEF cells. After treating cells with or without PRMT6-specific chemical inhibitor (PRMT6i) for 24 hrs, SIRT7 was immunoprecipitated and blotted with R388-specific methylation antibody"
NCBI gene IDs:
"SIRT7: 209011"
Figure legend: "H. Prmt6 depletion decreases R388 methylation of endogenous Sirt7. Scramble control cells (scr) or MEF cells stably expressing two different shRNAs (#1 and #2) against Prmt6 was established. R388 methylation of immunopurified endogenous Sirt7 was determined by western blot"
NCBI gene IDs:
"Prmt6: 99890"
Figure legend: "J-K. Prmt6 depletion reduces the percentage of R388-methylated endogenous SIRT7 in MEF cells. R388-methylated endogenous SIRT7 was captured from scramble control cells (scr) and Prmt6-knockdown MEF cells by using R388 site-specific methylation antibody (J). Input, output, and immunoprecipitate were analyzed by western blot to quantify the percentage of R388-methylated protein (K) (mean±SD, n=3 experimental replicates)"
NCBI gene IDs:
"Prmt6: 99890"
Figure legend: "A. RF, but not RK mutant, suppresses H3K18 deacetylase activity of SIRT7. Flag-SIRT7 and its RK/RF mutants were expressed in HEK293T and MEF cells, respectively. Cell lysates were subjected to western blot to detect histone H3K18 acetylation. H3K27ac and H3K56ac were included as negative control. The level of each histone acetylation marker was normalized against histone H3 (Ratio)"
NCBI gene IDs:
"SIRT7: 51547
SIRT7: 209011"
Figure legend: "B. R388 methylation of SIRT7 decreases its H3K18 deacetylase activity. HEK293T cells expressing wildtype SIRT7 or its RK/RF mutants were treated with or without AdOx for 24 hrs. The SIRT7 enzyme was immunopurified with Flag-beads and further incubated with extracted chromatin in vitro. R388 methylation of immunopurified SIRT7 and H3K18ac of in vitro deacetylated chromatin were determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "C. RF, but not RK mutant, shows reduced H3K18 deacetylase activity in vitro. Chromatin was extracted from HEK293T cells and incubated with recombinant GST-tagged SIRT7 (wildtype and RK/RF mutants) in vitro for 2 hrs. H3K18ac was determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "D. Wildtype PRMT6, but not its methylase-dead mutant, decreases the H3K18 deacetylase activity of SIRT7. Flag-tagged SIRT7 was co-expressed with wildtype PRMT6 or its catalytic-inactive mutant (DM) in HEK293T cells. The H3K18 deacetylase activity of immunopurified SIRT7 was assayed in vitro"
NCBI gene IDs:
"PRMT6: 55170
SIRT7: 51547"
Figure legend: "E. PRMT6 deficiency promotes the H3K18 deacetylase activity of SIRT7. Control sgRNA or two independent sgRNAs (#1 and #2) against PRMT6 was introduced into HEK293T cells. Flag-tagged SIRT7 was ectopically expressed in these cells. The histone deacetylase activity of immunoprecipitated SIRT7 was assayed in vitro using chromatin as a substrate. R388 methylation of immunopurified SIRT7 and H3K18 acetylation level of in vitro deacetylated chromatin was determined by western blot"
NCBI gene IDs:
"PRMT6: 55170
SIRT7: 51547"
Figure legend: "Wildtype SIRT7 or its RF mutant was re-expressed in Sirt7-knockdown MEF cells (A)"
NCBI gene IDs:
"SIRT7: 209011
Sirt7: 209011"
Figure legend: "Wildtype SIRT7, but not its RF mutant, restores mitochondria mas in Sirt7-deficient MEF cells Mitochondria mass was determined by Mito Tracker Red staining (B) (mean ± SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; n.s.=not significant, unpaired two-tailed t test)"
NCBI gene IDs:
"SIRT7: 209011
Sirt7: 209011"
Figure legend: "Wildtype SIRT7, but not its RF mutant, restore ATP leve in Sirt7-deficient MEF cells Cellular ATP was quantified and normalized to cell number (C) (mean ± SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; n.s.=not significant, unpaired two-tailed t test)"
NCBI gene IDs:
"SIRT7: 209011
Sirt7: 209011"
Figure legend: "Wildtype SIRT7, but not its RF mutant, restore cellular respiration in Sirt7-deficient MEF cells Respiration flux was determined as indicated (D). (mean ± SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; n.s.=not significant unpaired two-tailed t test)"
NCBI gene IDs:
"SIRT7: 209011
Sirt7: 209011"
Figure legend: "Wildtype SIRT7, but not its RF mutant, downregulates SIRT7 target genes involved in mitochondria biogenesis. Wildtype SIRT7 and its RF mutant were stably expressed in MEF cells (E)"
NCBI gene IDs:
"SIRT7: 209011"
Figure legend: "Wildtype SIRT7, but not its RF mutant, downregulates SIRT7 target genes involved in mitochondria biogenesis SIRT7 target gene expression was determined and presented as a heatmap on a log2 scale (F)"
NCBI gene IDs:
"SIRT7: 209011"
Figure legend: "Control cells and stable Sirt7-knockdown MEF cells were treated with or without 5 µM PRMT6i for 24 hrs. The H3K18ac level at the promoter of SIRT7 target genes (Gfm2, Mrps31, Mrps33, and Mrpl40) was determined by ChIP-qPCR, Rabbit IgG was used as a negative control (G) ( Data shown are mean±SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; ***p &lt; 0.001; n.s.=not significant, unpaired two-tailed t test"
NCBI gene IDs:
"Gfm2: 320806
Mrpl40: 18100
Mrps31: 57312
Mrps33: 14548
Sirt7: 209011"
Figure legend: "Inhibition of Prmt6 decreases mitochondria mas of control cells, but not Sirt7-knockdown MEF cells Control cells and stable Sirt7-knockdown MEF cells were treated with or without 5 µM PRMT6i for 24 hrs Mitochondria mass was determined by Mito Tracker Red staining (H) ( Data shown are mean±SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; ***p &lt; 0.001; n.s.=not significant, unpaired two-tailed t test"
NCBI gene IDs:
"Prmt6: 99890
Sirt7: 209011"
Figure legend: "Inhibition of Prmt6 decrease ATP leve of control cells, but not Sirt7-knockdown MEF cells. Control cells and stable Sirt7-knockdown MEF cells were treated with or without 5 µM PRMT6i for 24 hrs Cellular ATP was quantified and normalized to cell number (I) ( Data shown are mean±SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; ***p &lt; 0.001; n.s.=not significant, unpaired two-tailed t test"
NCBI gene IDs:
"Prmt6: 99890
Sirt7: 209011"
Figure legend: "Inhibition of Prmt6 decrease the respiration flux of control cells, but not Sirt7-knockdown MEF cells. Control cells and stable Sirt7-knockdown MEF cells were treated with or without 5 µM PRMT6i for 24 hrs Respiration flux was determined as indicated (J). ( Data shown are mean±SD, n=3 experimental replicates, *p&lt;0.05; **p&lt;0.01; ***p &lt; 0.001; n.s.=not significant, unpaired two-tailed t test"
NCBI gene IDs:
"Prmt6: 99890
Sirt7: 209011"
Figure legend: "A. Glucose depletion reduces SIRT7 methylation. MEF cells stably expressing Flag-SIRT7 were cultured in medium depleted with glucose (12 hrs), FBS (12 hrs) or glutamine (24 hrs). R388 methylation of immunopurified SIRT7 and Ampk phosphorylation were determined by western blot. R388 methylation level was normalized to Flag-SIRT7 (Ratio)"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "B. Glucose starvation decreases R388 methylation of SIRT7 in a time-dependent manner. MEF cells stably expressing Flag-SIRT7 were cultured in glucose-free media as indicated. R388 methylation level of immunopurified SIRT7 was determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "C. Glucose dose-dependently upregulates R388 methylation of SIRT7. MEF cells stably expressing Flag-SIRT7 were cultured with increasing concentrations of glucose as indicated for 12 hrs. R388 methylation of SIRT7 and Ampk phosphorylation were determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "D. AICAR treatment decreases R388 methylation of SIRT7. MEF cells stably expressing Flag-SIRT7 were treated with or without AICAR for 12 hrs. R388 methylation of immunoprecipitated SIRT7 was determined by western blot"
NCBI gene IDs:
"SIRT7: 51547"
Figure legend: "G. AMPK deletion increases SIRT7 R388 methylation. Flag-tagged SIRT7 was stably expressed in Ampk wildtype (WT) and Ampk α1/α2 double-knockout (DKO) MEF cells and 293A cells. SIRT7 protein was immunopurified with Flag-beads, R388 methylation was determined by western blot"
NCBI gene IDs:
"Ampk: 105787
SIRT7: 51547"
Figure legend: "H. AMPK is required for glucose starvation-induced R388 hypomethylation of SIRT7. Ampk wildtype (WT) and Ampk α1/α2 double-knockout (DKO) MEF cells stably expressing Flag-SIRT7 were cultured with or without glucose for 12 hrs. SIRT7 was purified with Flag-beads and R388 methylation was determined by western blot"
NCBI gene IDs:
"Ampk: 105787
SIRT7: 51547"
Figure legend: "I. PRMT6 is necessary for AMPK-induced SIRT7 hypomethylation. Flag-tagged SIRT7 was stably expressed in scramble control MEF cells or cells expressing two independent shRNAs targeting Prmt6 (#1 and #2). Cells were cultured with or without glucose for 12 hrs. R388 methylation of immunopurified SIRT7 was determined by western blot"
NCBI gene IDs:
"PRMT6: 99890
Prmt6: 99890
SIRT7: 51547"