EMBO/SourceData huggingface dataset:
Figure legend: "Western blotting on fractionated materials of OSCs before (siEGFP) and after (siWde) Wde depletion. Anti-Egg, anti-Wde, anti-Tub, and anti-H3 antibodies were used. H3 and Tub were detected as markers for nuclear (Nuc) and cytoplasmic (Cyto) fractions, respectively. Whole: whole OSC lysates."
NCBI gene IDs:
"EGFP: 
Wde: 36133"
Figure legend: "Immunofluorescence analysis of endogenous Egg (red) in OSCs upon Flag-Wde (green) co-expression. Anti-Egg and anti-Flag antibodies were used. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Flag: 
Wde: 36133"
Figure legend: "Immunofluorescence analysis of Myc-Egg WT (red) in OSCs upon Wde co-expression (green). Anti-Myc and anti-Flag antibodies were used. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Wde: 36133"
Figure legend: "Western blotting on fractionated materials of Myc-Egg-expressed OSCs before (-) and after Wde WT and Wde ∆CC co-expression. Anti-Myc, anti-Flag, anti-Tub, and anti-H3 antibodies were used. H3 and Tub were detected as markers for nuclear (Nuc) and cytoplasmic (Cyto) fractions, respectively. Whole: the whole OSC lysates."
NCBI gene IDs:
"Myc: 
Egg: 37962
Wde: 36133"
Figure legend: "Protein-protein interaction assays. Myc-Egg and Flag-Wde WT and its ∆CC mutant were expressed in OSCs. Immunoprecipitation was conducted using anti-Flag antibody while anti-Myc (upper) and anti-Flag (lower) antibodies were used for western blotting."
NCBI gene IDs:
"Flag: 
Myc: 
Egg: 37962
Wde: 36133"
Figure legend: "Immunofluorescence analysis of Myc-Egg WT (red) in OSCs upon Flag-Wde WT and ∆CC mutant (green) co-expression. Anti-Myc and anti-Flag antibodies were used. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Flag: 
Wde: 36133"
Figure legend: "Immunofluorescence analysis of Myc-Egg WT, Myc-Egg K987A, and Myc-Egg R988A mutants (red) in OSCs before and after Flag-Wde (green) co-expression. Anti-Myc and anti-Flag antibodies were used. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Flag: 
Wde: 36133"
Figure legend: "Western blotting of Myc-Egg WT and its K987A and R988A mutants. Flag-Wde: Flag-Wde was co-expressed. SV40-NLS: SV40-NLS was added to Myc-Egg. Anti-Myc and anti-Flag antibodies were used."
NCBI gene IDs:
"Flag: 
Myc: 
Egg: 37962
Wde: 36133"
Figure legend: "Protein-protein interaction assays. Myc-Egg WT, Myc-SV40-NLS-Egg WT, and Myc-SV40-NLS-Egg K1085R mutant were expressed with Flag-Wde in OSCs. Immunoprecipitation was conducted using anti-Flag antibody while anti-Myc (upper) and anti-Flag (lower) antibodies were used for western blotting."
NCBI gene IDs:
"Flag: 
Myc: 
Egg: 37962
Wde: 36133"
Figure legend: "Rescue assays. Myc-Egg WT and Myc-SV40-NLS-Egg WT were expressed in OSCs upon endogenous Egg and Wde depletion (siEgg and siWde). Western blotting was performed using anti-Egg (left) and anti-Myc (right) antibodies. RT-qPCR quantified the expression level of Stalker2 transposon. The act5c gene was used for normalization. siEGFP: siRNA for EGFP was used as a negative control. Error bars represent SD from three independent experiments. *p &lt; 0.05 (unpaired Student's t-test)."
NCBI gene IDs:
"act5c: 
Stalker2: 
EGFP: 
Myc: 
Egg: 37962
Wde: 36133"
Figure legend: "Western blotting of Myc-SV40-NLS-Egg WT in whole (Whole), cytoplasm/nucleoplasmic (Cyto/Np) and chromatin (Chromatin) fractions before (-) and after Flag-Wde co-expression. Anti-Myc and anti-Flag antibodies were used. Histone H3 was detected as a marker of chromatin fraction."
NCBI gene IDs:
"Flag: 
Wde: 36133"
Figure legend: "Immunofluorescence analysis of H3K9me3 (green), Myc-Egg WT (red), and Myc-SV40-NLS-Egg WT (red) in OSCs before and after Flag-Wde (yellow) co-expression. Anti-H3K9me3, anti-Myc and anti-Flag antibodies were used. DAPI (blue) shows the nuclei. Scale bar: 10 μm."
NCBI gene IDs:
"Flag: 
Wde: 36133"
Figure legend: "Heatmaps of Egg ChIP-seq signal within 10 kb windows centered on Egg peaks in control (siEGFP) and Wde-depleted (siWde) OSCs."
NCBI gene IDs:
"EGFP: 
Wde: 36133"
Figure legend: "ChIP-qPCR was performed before (siEGFP) and after (siWde) Wde depletion. qPCR was performed for Piwi target transposons (Stalker2, mdg1, and gypsy) and normalized to %input of a PIwi non-target transposon (roo). Error bars represent SD from three independent experiments."
NCBI gene IDs:
"Stalker2: 
EGFP: 
gypsy: 
mdg1: 
roo: 
Wde: 36133"
Figure legend: "Density plots for Egg ChIP-seq signal on Stalker2, mdg1, and gypsy in control (siEGFP) and Wde-depleted (siWde) OSCs. Input: input DNA was used as a control. RPM: reads per million mapped reads."
NCBI gene IDs:
"gypsy: 
EGFP: 
mdg1: 
Stalker2: 
Wde: 36133"
Figure legend: " Mice were infected with sub-lethal dose (25 pfu) of influenza virus and assessed as follows (B) Relative amount of PR8 viral NP mRNA, by qPCR Data information: Data are representative of three independent experiments with 3-5 mice per group and represented as mean ± S.E.M "
NCBI gene IDs:
"NP: 956531"
Figure legend: "Mice were superinfected as described in Fig 3A. (A) Bacterial load as determined by enumeration of cfu in BAL following administration of αIL-17 or αIL-22 blocking antibody. Control wild-type and T-bet-/- groups were administered with IgG isotype control antibody Data information Dat are combined from two independent experiments (n = 8-12 mice per group) All data are represented as mean ± S.E.M. Statistical significance was determined with Kruskal-Wallis test followed by Dunn\"s multiple comparisons test n.s. = not significant"
NCBI gene IDs:
"T-bet: 57765"
Figure legend: "Mice were superinfected as described in Fig 3A (B) T-bet-/- mice were administered αIL-17 or IgG isotype control antibody every other day from day -2 post-secondary bacterial infection and monitored for survival Data information: Dat are combined from two independent experiments (n = 8-12 mice per group) All data are represented as mean ± S.E.M. Statistical significance was determined wit logrank test in (B"
NCBI gene IDs:
"T-bet: 57765"
Figure legend: "Mice were superinfected as described in Fig 3A (D) Pneumococcal cfu in BAL following αLy6G antibody mediated neutrophil depletion. Control T-bet-/- mice were administered with IgG isotype control antibody Data information: Dat are combined from two independent experiments (n = 8-12 mice per group) All data are represented as mean ± S.E.M. Statistical significance was determined wit Mann-Whitney U test in (D"
NCBI gene IDs:
"T-bet: 57765"
Figure legend: "The RNA-seq data of CTH was downloaded from the TCGA database (H) The mRNA expression of CTH in PC specimens and normal tissues. Data are presented as means ± SD (n=50 cases for adjancent non-tumor parts and 469 cases for PC tumors). Student's t-test was used for the statistical analysis (**P&lt;0.01)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(I) Kaplan-Meier survival analysis of PC patients with high or low CTH expression. The statistical significance was determined using the χ2 test."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(A) Western blot analysis of the CTH level in PC3-T2 cells with CTH overexpression by pCMV-CTH-HA or PC3-B2 cells with CTH knockdown by siCTH-1."
NCBI gene IDs:
"HA: 
CTH: 1491"
Figure legend: "(B) Top: The H2S production capacity of cell lysates from PC3-T2 cells with CTH overexpression by pCMV-CTH-HA or PC3-B2 cells with CTH knockdown by siCTH-1. Lead acetate-soaked paper strips show a PbS brown stain as a result of reaction with H2S. Bottom: The level of H2S production was quantified by densitometry, and the histograms represent the means ± SD (n=3 biological replicates). ANOVA followed by Tukey's post-hoc test was used for the statistical analysis (*P&lt;0.05)."
NCBI gene IDs:
"HA: 
CTH: 1491"
Figure legend: "(C) PC3-B2 cells were transfected with control or siCTH-1 and then were subjected to migration (16h) and invasion (24h) assays. Data information: Data shown represent the means ± SD (n=3 biological replicates). Student's t-test was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(D) Cell proliferation rates of PC3-B2 cells with CTH knockdown. Cell proliferation assay was performed by MTS reagent. Data information: Data shown represent the means ± SD (n=3 biological replicates). Student's t-test was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(E) Western blot analysis of the CTH levels in PC3 cells transfected with HA-CTH or pCMV-HA vector control."
NCBI gene IDs:
"HA: 
CTH: 1491"
Figure legend: "(F) PC3 cells were transfected with HA-CTH or pCMV-HA vector control and then incubated for migration (16h) and invasion assay (24h). Data information: Data shown represent the means ± SD (n=3 biological replicates). Student's t-test was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"HA: 
CTH: 1491"
Figure legend: "(A) Real-Time RT-PCR analysis for IL-1β mRNA levels in PC3 cells with control or CTH knockdown. Recombinant IL-1β (10ng/ml) was added for 24h in serum-free medium. Data information: All data shown represent the means ± SD (n=3 biological replicates). ANOVA followed by Tukey's post-hoc test was used for the statistical analysis. (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491
IL-1β: 3553"
Figure legend: "(B) ELISA assay for IL-1β protein level in the culture medium of PC3 cells with control or CTH knockdown. Data information All data shown represent the means ± SD (n=3 biological replicates). Student's t-test (B) was used for the statistical analysis. (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(C) PC3 cells transfected with control or CTH knockdown were incubated for invasion (24h) assay with recombinant IL-1β (10ng/ml) added to the medium of the lower chamber. Data information: All data shown represent the means ± SD (n=3 biological replicates). ANOVA followed by Tukey's post-hoc test was used for the statistical analysis. (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(D) PC3 cells transfected with control or CTH siRNA were exposed to IL-1β (20ng/ml) for 1hr. Subcellular localization of p65 was detected by immunocytochemistry. Nuclei were counterstained with DAPI. The representative images are shown. Scale bars: 25μm."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "Real-Time RT-PCR analysis for VEGF (F) mRNA level in PC3 cells with control or CTH knockdown. Recombinant IL-1β (10ng/ml) was added for 24h in serum-free medium. Data information: All data shown represent the means ± SD (n=3 biological replicates). ANOVA followed by Tukey's post-hoc test was used for the statistical analysis. (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491
VEGF: 7422///7423"
Figure legend: "Real-Time RT-PCR analysis for MMP-13 (G) mRNA level in PC3 cells with control or CTH knockdown. Recombinant IL-1β (10ng/ml) was added for 24h in serum-free medium. Data information: All data shown represent the means ± SD (n=3 biological replicates). ANOVA followed by Tukey's post-hoc test was used for the statistical analysis. (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)."
NCBI gene IDs:
"CTH: 1491
MMP-13: 4322"
Figure legend: " (B) PC3 cells transfected with control or CTH siRNA were incubated for invasion (24h) assay. DMEM/10% FBS, with or without NaHS (1μM), acting as a chemoattractant Data information Data shown represent the means ± SD (n=3 biological replicates) ANOVA followed by Tukey's post-hoc tes was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)"
NCBI gene IDs:
"CTH: 1491"
Figure legend: " (E) Real-Time RT-PCR analysis for IL-1β, VEGF, and MMP-13 mRNA levels in PC3 cells treated with 1μM NaHS for 24h Data information Data shown represent the means ± SD (n=3 biological replicates) Student's t-tes was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)"
NCBI gene IDs:
"IL-1β: 3553
MMP-13: 4322
VEGF: 7423///7422"
Figure legend: " (G) Western blot analysis of the p65 expression in PC3 cells with p65 knock-out and then transfected with p65 wild-type or C38S mutant Data information Data shown represent the means ± SD (n=3 biological replicates)"
NCBI gene IDs:
"p65: 5970"
Figure legend: " (H) PC3 cells with p65 knock-out with or without overexpression of p65 wild-type or C38S mutant were incubated for invasion assay for 24 hrs. DMEM/10% FBS was used as a chemoattractant Data information Data shown represent the means ± SD (n=3 biological replicates) ANOVA followed by Tukey's post-hoc tes was used for the statistical analysis (*P&lt;0.05; **P&lt;0.01; ***P&lt;0.001)"
NCBI gene IDs:
"p65: 5970"
Figure legend: "1x106 PC3 cells with p65 knock-out were transiently re-expressed p65 wild-type or C38S mutant and inoculated into the bloodstream via tail-vein injection. After 7 days of inoculation, mouse lung was collected to examine lung metastasis. (I) Images show H&amp;E staining of the lungs from mice injected with p65 knockout PC3 cells re-expressing p65 wild-type or C38S mutant. Red arrows indicate metastatic nodules. Scale bar: 1mm"
NCBI gene IDs:
"p65: 5970"
Figure legend: "1x106 PC3 cells with p65 knock-out were transiently re-expressed p65 wild-type or C38S mutant and inoculated into the bloodstream via tail-vein injection. After 7 days of inoculation, mouse lung was collected to examine lung metastasis The total metastatic nodules per lung (J were quantified. Data are presented as means ± SEM (n=7 mice per group). Student's t-test was used for the statistical analysis (*P&lt;0.05)"
NCBI gene IDs:
"p65: 5970"
Figure legend: "1x106 PC3 cells with p65 knock-out were transiently re-expressed p65 wild-type or C38S mutant and inoculated into the bloodstream via tail-vein injection. After 7 days of inoculation, mouse lung was collected to examine lung metastasis percentage of the metastatic area per lung (K) were quantified. Data are presented as means ± SEM (n=7 mice per group). Student's t-test was used for the statistical analysis (*P&lt;0.05)"
NCBI gene IDs:
"p65: 5970"
Figure legend: "(A) Western blot analysis of the CTH expression in PC3 cells stably expressing control or CTH shRNA."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "1x106 PC3 cells with control or CTH knockdown were orthotopically injected into the mouse prostate for 60 days. The tumor weight (B), and tumor volume (C) were compared. Data are presented as means ± SEM (n = 10 mice per group). Data information: Student's t-test was used for the statistical analysis (*P&lt;0.05)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "1x106 PC3 cells with control or CTH knockdown were orthotopically injected into the mouse prostate for 60 days. Incidences of paraaortic lymph nodes metastasis (D) and bone metastasis (E) are shown. Data are presented as means ± SEM (n = 10 mice per group). Data information: Student's t-test was used for the statistical analysis (*P&lt;0.05)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(F) Real-Time RT-PCR analysis for CTH mRNA levels in PC3 orthotopic tumors from shCon or shCTH group. Data shown represent normalized means ± SEM (n=10 mice per group). Data information: Student's t-test was used for the statistical analysis (*P&lt;0.05)."
NCBI gene IDs:
"CTH: 1491"
Figure legend: "(C) TFR1 mRNA levels in  muscle biopsies from cancer-patients of late stage  cachexia (19 healthy subjects, 17  cachectic patients with at least 10% total body weight loss) Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two-tailed Student's T-test. Data are mean ± SEM. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"TFR1: 7037"
Figure legend: "(F) TFR1  mRNA levels in the gastrocnemius of  mice subjected to  iron deprivation by feeding with an  iron deficient diet (IDD) combined to a phlebotomy  (PHL) (n=5-6) Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two-tailed Student's T-test. Data are mean ± SEM. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"TFR1: 22042"
Figure legend: "(G) Cross-sectional area of skeletal muscle fiber  transfected with shSCR (scramble) and shTFR1 (n=3-4) and representative picture of a shTFR1 transfected  fiber. Scale bar=50µm. (H) Cross-sectional area of skeletal muscle fibers  transfected with TFR-pHuji  (n=4) and representative picture of a  TFR-pHuji  transfected  fiber. Scale bar=50µm. Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two-tailed Student's T-test. Data are mean ± SEM. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"pHuji: 
TFR: 7037
TFR1: 7037"
Figure legend: "(C-D) mRNA levels of mitochondrial iron  importer  MFRN2 (C) and the rate limiting enzyme of heme synthesis ALAS2 (D) normalized to  18s in mouse gastrocnemius (n=4-5). Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two-tailed Student's T-test. Data are mean ± SEM. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"18s: 
ALAS2: 11656
MFRN2: 246696"
Figure legend: "(I-K) mRNA levels of Murf 1  (I), Atrogin 1 (J), and REDD1  (K) normalized to GAPDH in  gastrocnemius  (n=5-14). Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by one-way Anova with Bonferroni's correction, Data are mean ± SEM. ∗p &lt; 0.05 and ∗∗∗p &lt; 0.001 compared to control and #p &lt; 0.05, ##p &lt; 0.01, ###p &lt; 0.001 compared to  C26  untreated group."
NCBI gene IDs:
"REDD1: 74747
Atrogin 1: 67731
GAPDH: 14433
Murf 1: 433766"
Figure legend: "(F-H) mRNA levels of  ACOT 1 (F),  MCD (G), and CPT1B (H) normalized to GAPDH in gastrocnemius (n=5-10). Data information: For all data, n represents the number of biological replicates. Statistical significance was calculated by unpaired, two-tailed Student's T-test. Data are mean ± SEM. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001 compared to control and #p &lt; 0.05, ##p &lt; 0.01, ###p &lt; 0.001 compared to C26 untreated group."
NCBI gene IDs:
"ACOT 1: 26897
CPT1B: 12895
GAPDH: 14433
MCD: 56690"
Figure legend: "(A) Brigthfield images of human patient derived organoids (PDOs) from gastric cancer with and without RTK/MAPK alterations (scale bar 100µm, zoom in 40µm)."
NCBI gene IDs:
"RTK: 780///4914
MAPK: 5602///5601///5599///5598///5597///5596///5595///5594"
Figure legend: "(B) Drug response curves of PDOs upon treatment with the MEK1/2 inhibitor trametinib (biological replicates n=3, data are presented as mean, SD values are shown in Table EV2). (C) Drug response curves upon HDAC inhibition with panobinostat (PAN) and entinostat (ENT) (biological replicates n=3, data are presented as mean, SD values are shown in Table EV2). (D) Comparison of the relative area under the curve (AUCrel) upon trametinib treatment in RTK/MAPK altered (mean AUCrel: 0.5308; biological replicate n=7) vs. non‑altered (mean AUCrel: 0.7101; biological replicates n=6) human PDOs (two‑tailed Student's t‑Test; *p= 0.0356)."
NCBI gene IDs:
"RTK: 780///4914
MAPK: 5602///5601///5599///5598///5595///5594///5597///5596"
Figure legend: "A. Structure of Brca1ex2 allele. * Note that exon 4 was annotated in error in original descriptions of the gene structure and is not drawn. RT-PCR shows a novel product from the Brca1ex2 allele, corresponding to splicing of exon 1 directly to exon 3."
NCBI gene IDs:
"Brca1: 12189"
Figure legend: "B. Western blot to detect BRCA1 and BARD1 in MEFs expressing WT and mutant Brca1."
NCBI gene IDs:
"Brca1: 12189"
Figure legend: "C. Immunofluorescent (IF) detection of BRCA1 and RAD51 at IR-induced nuclear foci in cells expressing WT and mutant forms of Brca1. Scale bar: 10 ingD. Quantification of IF, showing proportion of cells with RAD51 foci that also had &gt;5 BRCA1 foci. N=2.E. Quantification of IF, showing proportion of RAD51 foci that colocalized with BRCA1 foci. N=2."
NCBI gene IDs:
"Brca1: 12189"
Figure legend: "F. Immunofluorescent detection of BARD1 at IR-induced nuclear foci in MEFs expressing WT and mutant forms of Brca1. Scale bar: 10 μ S"
NCBI gene IDs:
"Brca1: 12189"
Figure legend: "A. Western blot showing abundance of BRCA1 and BARD1 protein in Brca1Δ2/Δ2;Trp53bp1-/- mouseB cells."
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "B. Analysis of chromosome aberrations in mice with targeted deletion of Brca1 exon 2 and Trp53bp1. Left, metaphase spreads were prepared from B cells treated with olaparib. Arrows show examples of chromosome aberrations. Right, quantification of chromosome breaks (CSB), chromatid-type breaks (CTD), radial chromosomes, and other abnormalities in mouseB cells. Error bars indicate SD, N=3"
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "A. Kaplan-Meier survival curve for Brca1ex2/+;Trp53bp1-/- and Brca1ex2/ex2;Trp53bp1-/- mice. N=20 animals in each group. Statistical analysis by Mantel-Cox log-rank test."
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "B. Kaplan-Meier survival curve for Brca1ex2/+;Trp53bp1-/-;Trp53+/- and Brca1ex2/ex2;Trp53bp1-/-;Trp53+/- mice. N=20 animals in each group. Statistical analysis by Mantel-Cox log-rank test."
NCBI gene IDs:
"Brca1: 12189
Trp53: 22059
Trp53bp1: 27223"
Figure legend: "C. Percentage of Brca1ex2/+;Trp53bp1-/- and Brca1ex2/ex2;Trp53bp1-/- mice showing signs of abnormal tissue morphology at death. N=46 animals inspected for Brca1ex2/+;Trp53bp1+/+, N=54 animals inspected for Brca1ex2/ex2/Trp53bp1-/-."
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "D. Tissues affected by abnormal growth from N=46 Brca1ex2/+/Trp53bp1-/- and N=54 Brca1ex2/ex2/Trp53bp1-/- mice."
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "A. H&amp;amp;amp;E-stained sections of seminiferous tubules from testes of WT and Brca1ex2/ex2/Trp53bp1-/- male mice. Scale bar: 100 μm."
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "B. Immunofluorescence on meiotic chromosomes from WT and Brca1ex2/ex2;Trp53bp1-/-spermatocytes. Spreads were stained for SYCP3 to indicate chromosome axes and extent of synapsis and were stained for SYCP3 to indicate chromosome axex body. Scale bar: 5 apsi"
NCBI gene IDs:
"Brca1: 12189
Trp53bp1: 27223"
Figure legend: "(G) LMP1 binds  directly to ANT1 dependent on its transmembrane domain in 293T  cells.  IP/IB was used to detect the interaction between Flag-tagged   LMP1  mutants and Myc-tagged  ANT1. IP  of cell lysates using a  Flag or Myc antibody."
NCBI gene IDs:
"Flag: 
Myc: 
LMP1: 3783750
ANT1: 291"
Figure legend: "(E) Wild-type (WT) and Ews-/- embryos mid-brain at E17.5 days were subjected to TUNEL assay. Scale bar indicates 100 μm (lnsert)."
NCBI gene IDs:
"Ews: 14030"
Figure legend: "(A) Western blot analysis of Parp1 and DNA damage markers in Wild-type (WT) and Ews-/- mBA cells. Cells were treated with MMS (0.02%) in a time dependent manner. Proteins were fractionated into two groups, chromatin-bound (Chro) proteins and soluble (Solu) proteins. (B) After treatment with MMS (0.02%, 1hour), the media was replaced to release the DNA damage. The proteins were fractionated and the specific protein kinetics at chromatin was analyzed by western blot."
NCBI gene IDs:
"Ews: 14030"
Figure legend: "(C) GFP-PARP1 U2OS cells were transfected with siControl and siEWS. Local DNA damage was induced by micro-irradiation using a 405nm laser. Data represented as mean ±SEMs and more than six cells were analyzed from six independent experiments. The statistical significance determined by One-way ANOVA, ***P&lt; 0.001. Scale bar indicates 5 μm."
NCBI gene IDs:
"EWS: 2130"
Figure legend: "(D) Fluorescence correlation spectroscopy (FCS) was measured after 15 minutes later following micro-irradiation (Upper) or under normal condition in GFP-PARP1 U2OS cells with either siControl or siEWS. Data represented as mean ±SEMs, more than 50 cells were analyzed, and significance determined by One-way ANOVA, ***P&lt; 0.001. Scale bar indicate 5 μm."
NCBI gene IDs:
"EWS: 2130"