EMBO/SourceData huggingface dataset:
Figure legend: "Three types of GSC (GBM4, GBM6, and GBM8) were transfected with miR-10bASO, and gene expression was analyzed 24 hours later by the Affymetrix microarrays. The heatmaps colors intensity demonstrate altered expression of the genes (up- or down-regulated relative to the mock treated samples) with the fold change &gt; 1.2 and p &lt; 0.05 in at least two out of the three GSC cultures.A. The genes associated with \"cell cycle\" bioterm have been selected using Ingenuity Pathway Analysis. The treatment with the miR-10b inhibitor is indicated as \"miR-10b-i\"."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "B. The genes associated with \"RNA splicing\" bioterm have been selected using Gene Ontology (GO). The treatment with the miR-10b inhibitor is indicated as \"miR-10b-i\". Arrows depict the genes selected as candidate direct targets for further study."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "C. miR-10b binding motifs are enriched in 5'UTRs of the genes up-regulated by miR-10b ASO. The graph shows the probability that enrichment of the miR-10b motifs in mRNAs up-regulated vs. unchanged (p&lt;0.05) by anti-miR-10b does not occur by chance."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "D. The miR-10b octamer motifs' composition of the 5'UTRs was compared between transcripts up- and down-regulated on the microarrays. The relative frequencies of various miR-10b binding motifs are shown, indicating that mostly miR-10b 3'-end binding motifs are enriched in the up-regulated mRNAs."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "Figure 3.Expression analysis of splicing factor mRNAs in various GBM datasets.A. The genes encoding splicing factors down-regulated by miR-10b are expressed at lower levels in various GBM datasets relative to their expression in normal brain tissues.B. In contrast, many splicing factors up-regulated by miR-10b are overexpressed in the GBM datasets.Six high-content GBMmicroarray datasets from the Oncomine resource (https://www.oncomine.org/resource/main.html), including TCGA_BrainGBM (2), Bredel Brain2 (31), Lee Brain (32), Liang Brain (33), Murat Brain (34) and Sun Brain (35), that collectively contain information for 858 GBM and 52 control samples, have been utilized for the analysis. The data is presented as log2 fold change between GBM and normal brain tissues."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "B. qRT-PCR analysis validates that mRNA of MBNL1-3, SART3, RSRC1 and other splicing factors are de-repressed by miR-10b ASO in different GSC and GBM cell lines. mRNA expression levels were normalized to GAPDH expression."
NCBI gene IDs:
"GAPDH: 
MBNL1: 4154
miR-10b: 406903
RSRC1: 51319
SART3: 9733"
Figure legend: "C. Regulation of representative splicing-related proteins by miR-10b mimic in GSC, as demonstrated by Western blot analysis. The signals were quantified using ImageJ and normalized to beta-actin. The ratios between miR-10b mimic expressing and control samples are indicated."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "D. miR-10b mimic regulates 5'UTR Luciferase reporter containing a single miR-10b complementary site."
NCBI gene IDs:
"Luciferase: 
miR-10b: 406903"
Figure legend: "E. miR-10b mimic regulates 5'UTR Luciferase reporters of some splicing factors genes bearing wild type (WT), but not mutated (Mut) miR-10b binding sites. Statistical significance of the differences was determined by Student's t test, with p-values &lt; 0.05 indicated by asterisks, p &lt; 0.01 by two asterisks, and p&lt; 0.001 by three asterisks. Numbers of replicates and exact P-values are included in Appendix Table S4."
NCBI gene IDs:
"Luciferase: 
miR-10b: 406903"
Figure legend: "B. 2'-O-MOE-POmiR-10b inhibitor (miR-10b-i) or non-targeting control (1 μg of each) formulated with InVivo-jet PEI were injected intratumorally at days 20 and 25 after cells implantation. The efficacy of miR-10b inhibition was assessed by qRT-PCR analysis of the resected tumors, with miR-10b expression levels normalized to miR-125b."
NCBI gene IDs:
"miR-125b: 
miR-10b: 406903"
Figure legend: "C. qRT-PCR analysis demonstrates that miR-10b inhibition in orthotopic GBM8 leads do de-repression of its mRNA targets. mRNA expression levels were normalized to GAPDH."
NCBI gene IDs:
"GAPDH: 
miR-10b: 406903"
Figure legend: "D. Inverse correlation between miR-10b levels and expression of its mRNA targets in resected GBM8 tumors."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "E. Inhibition of miR-10b markedly reduces tumor burden. The left panels illustrate tumor imaging in representative animals at day 29. The bars represent average signal ratios for each group at day 29 (after treatment) to day 20 (at the beginning of treatment). N = 7 animals per group at treatment initiation."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "B-D. Systemic inhibition of miR-10b markedly reduces tumor burden. Uncomplexed 2'-O-MOE-PSmiR-10b inhibitor (miR-10b-i) or non-targeting control of the same chemistry were injected at 80 mg/kg through the tail vein at the days indicated by arrows.B. The left panels illustrate tumorimages of representative animals at day 34, and average signals (photons/sec) are indicated below the images. The bars represent average signal ratios for each group at day 34, relative to day 6"
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "B-D. Systemic inhibition of miR-10b markedly reduces tumor burden. Uncomplexed 2'-O-MOE-PSmiR-10b inhibitor (miR-10b-i) or non-targeting control of the same chemistry were injected at 80 mg/kg through the tail vein at the days indicated by arrows.B. The left panels illustrate tumorimages of representative animals at day 34, and average signals (photons/sec) are indicated below the images. The bars represent average signal ratios for each group at day 34, relative to day 6.C. Each mouse was sacrificed when the tumor-generated signal reached 5X107 photons/sec, and Kaplan-Meier survival plots were built retrospectively.D. Growth curves of individual tumors based on the ratios of bioluminescence signals to the baseline signals at day 6."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "E. The efficacy of miR-10b inhibition in intracranial tumors was assessed by qRT-PCR analysis of the resected tumor tissues, with miR-10b expression levels normalized to miR-125b."
NCBI gene IDs:
"miR-125b: 
miR-10b: 406903"
Figure legend: "F. qRT-PCR analysis demonstrates that miR-10b targets were de-repressed in orthotopic GBM8 upon systemic administration of the miR-10b inhibitor. Seven tumors per condition, and two specimens per tumor have been analyzed. mRNA expression levels were normalized to GAPDH."
NCBI gene IDs:
"GAPDH: 
miR-10b: 406903"
Figure legend: "G. Inverse correlation between miR-10b levels and expression of its mRNA targets in resected GBM8 tumors."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "B-D. Systemic treatment of intracranialGBM8tumors with uncomplexed 2'-O-MOE-PSmiR-10b inhibitor (miR-10b-i) or non-targeting control oligonucleotide (at 80 mg/kg) was not associated with toxic effects.B. No significant difference in average mice weight was observed between the anti-miR-10b and control treatment groups.C. No significant difference in average organs' weight was observed between the anti-miR-10b and control treatment groups.D. No significant difference in tissue histology using Hematoxylin and Eosin staining was observed between the anti-miR-10b and control treatment groups."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "A. Continuous osmotic delivery of miR-10b inhibitor markedly reduces tumor burden. The osmotic pumps, loaded with lipid nanoparticles formulated with 2'-O-MOE-POmiR-10b inhibitor or non-targeting control infused 2 μg of the oligonucleotides per day introtumorally, over 13 days. Tumors growth was monitored by the WBI, and the left panels illustrate tumor imaging of representative animals at the end of treatment. The bars represent average signal ratios for each group at day 13 (end of treatment), relative to day 2 (treatment initiation)."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "F. Quantitative immunostainings analysis indicates that proliferation and apoptosis markers are affected by anti-miR-10b treatment. No significant effect on invasion of intracranial GBM8 was observed."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "A, B. miR-10b inhibition decreases GL261 cellviability. Cellviability was measured at days 3-7 after transfection with miR-10b inhibitor, non-targeting control, or Lipofectamine 2000 alone (mock).A. Phase contrast photographs of GL261 cultures at day 6 post-transfection.B. Growth curves of cultured GL261 cells, based on the viability assay."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "C. InVivo-jet PEI - formulated 2'-O-MOE-PS/PO miR-10b inhibitor or non-targeting control were infused to orthotopic GL261 tumors by osmotic pumps, starting at day 6 after cell implantation. The uptake of ASOs was confirmed by IHC for PS-containing oligonucleotides (green). GL261 tumor cells expressing M-Cherry are red."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "D. Osmotic delivery of miR-10b inhibitor markedly reduces GL261tumorgrowth in immunocompetent Black 6 Albino mice. Micephotographs show tumor imaging in representative animals at day 3 after pump implantation, and average signals in photons per second are indicated. Tumorgrowth rates were calculated as ratios of the signals at day 3 of the treatment to day 1 prior to initiation of the treatment."
NCBI gene IDs:
"miR-10b: 406903"
Figure legend: "F. qRT-PCR analysis demonstrates that miR-10b target p21 was de-repressed in GL261 tumors upon miR-10b inhibition. mRNA expression levels were normalized to GAPDH."
NCBI gene IDs:
"GAPDH: 
p21: 12575
miR-10b: 406903"
Figure legend: "A-C AID expression was analyzed in colon and pancreatic human cell lines (A) qRT-PCR analysis of AID expression in LoVo and SW480 colon cell lines. n = 5 (LoVo); 3 (SW480). **P-value: LoVo: 0.0017; SW480: 0.0079. (B) qRT-PCR analysis of AID expression in AsPC and PaTU-8988S pancreatic cell lines (n = 2). *P-value: AsPC: 0.0369; PaTU-8988S: 0.0119."
NCBI gene IDs:
"AID: 57379"
Figure legend: "A-C AID expression was analyzed in pancreas explants from C57BL/6 mice. Samples were treated as indicated with 50 ng/ml TNF-α. (C) qRT-PCR analysis of AID expression in pancreatic explants from wild-type mice (n = 3). *P = 0.0242."
NCBI gene IDs:
"AID: 11628"
Figure legend: "D AID−/− or AID+/− mice were treated with 3% DSS for 10 cycles, and colonic sections were analyzed by histology inspection after H/E staining. Graphs represent mean frequency values of adenoma and adenocarcinoma lesions of five independent experiments. n = 28 (AID−/− males); 35 (females); 23 (AID+/− males); 25 (females). P-value: male: 0.8; female: 0.246."
NCBI gene IDs:
"AID: 11628"
Figure legend: "GFP immunofluorescence in colonic and pancreatic tissue from R26AID+/KIVillinCRE+/TG and R26AID+/KIp48CRE+/KI mice. Scale bar: 50 μm."
NCBI gene IDs:
"CRE: 
AID: 11628
p48: 16391
Villin: 22349"
Figure legend: "qRT-PCR analysis of AID expression in colonic and pancreatic tissue from R26AID+/KIVillinCRE+/TG and R26AID+/KIp48CRE+/KI mice. n = 5 (R26AID+/+ VillinCRE+/TG); 4 (R26AID+/KIVillinCRE+/TG); 2 (R26AID+/+p48CRE+/KI); 2 (R26AID+/KIp48CRE+/KI). LPS+IL4-stimulated B cells are shown as a positive control (n = 2). Bars show mean values ± SEM normalized to LPS+IL4-treated B cells."
NCBI gene IDs:
"AID: 11628
p48: 16391
Villin: 22349"
Figure legend: "Kaplan-Meier survival curves for R26AID+/KIVillinCRE+/TG (left) (n = 47 (R26AID+/+ VillinCRE+/TG); 38 (R26AID+/KIVillinCRE+/TG)) and R26AID+/KI p48CRE +/KI mice (right) (n = 39 (R26AID+/+p48CRE+/KI); 23 (R26AID+/KIp48CRE+/KI))."
NCBI gene IDs:
"AID: 11628
p48: 16391
Villin: 22349"
Figure legend: "A, B Analysis of AID mutagenic activity in Elastase1 (A) and Elastase2a (B) by next-generation sequencing. Pancreatic DNA was isolated from pools of R26AID+/KIp48CRE+/KI and control R26AID+/+p48CRE+/KI 20-week-old mice, and then PCR-amplified with specific primers and sequenced as previously described (Perez-Duran et al, 2012). Graphs show cytosine mutation frequency overall (total) or at AGCT hotspots. n = 2. *P-value: Elastase1: 0.0382; Elastase2a: 0.009."
NCBI gene IDs:
"AID: 11628
Elastase1: 109901
Elastase2a: 13706
p48: 16391"
Figure legend: "C HTM-mediated quantification of γH2AX intensities per nuclei in pancreas explants cultured in vitro for 6 days. Red lines show mean values. Results of two independent experiments are shown. ****P &lt; 0.0001.D Representative images of γH2AX staining in pancreas explants from R26AID+/+p48CRE+/KI (top) or R26AID+/KIp48CRE+/KI mice (bottom) (40× magnification). White arrow points a representative positive cell."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Representative images of Ki67 staining in pancreas from aged (75-week-old) R26AID+/+p48CRE+/KI (top) or R26AID+/KIp48CRE+/KI mice (bottom). Detail is shown on the right. Scale bar: 100 μm. Graph shows quantification of Ki67-positive epithelial cells per mm2 of tissue (n = 8). *P = 0.0266."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Representative immunofluorescence staining of 20-week-old R26AID+/KIp48CRE+/KI mice: blue, DAPI; red, Ki67; green, CK8. Scale bar: 50 μm. Detail is shown on the bottom."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Quantitative FACS analysis of RAE expression in pancreatic explants from R26AID+/+p48CRE+/KI and R26AID+/KIp48CRE+/KI mice. Left: Graph shows mean fluorescence intensity. Each dot represents an individual mouse. n = 9 (R26AID+/+p48CRE+/KI); 8 (R26AID+/KIp48CRE+/KI). P = 0.077. Right: Representative FACS staining for RAE in explants from R26AID+/+p48CRE+/KI (red) and R26AID+/KIp48CRE+/KI mice (black)."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Analysis of RAE expression by ddPCR in aged (75-week-old) mice. Data are presented as the percentage of positive drops normalized to the mean control value. Each point represents an individual mouse and shows the mean amplification from two independent experiments n = 7 (R26AID+/+p48CRE+/KI); 9 (R26AID+/KIp48CRE+/KI). *P = 0.012."
NCBI gene IDs:
"RAE: 
AID: 11628
p48: 16391"
Figure legend: "Analysis of killing activity. Primary explants of pancreatic cells from R26AID+/+p48CRE+/KI or R26AID+/KIp48CRE+/KI mice were cultured with primary NK cells, and killing activity was assessed as described in Materials and Methods (n = 4). P = 0.19."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Hematoxylin-eosin (HE) staining of pancreas from aged (75-week-old) R26AID+/+p48CRE+/KI and R26AID+/KIp48CRE+/KI mice. Left: Representative HE staining showing an immune infiltrate in a R26AID+/KIp48CRE+/KI mouse. Scale bar: 200 μm. Right: Quantification of number of foci per mm2 of tissue. n = 13 (R26AID+/+p48CRE+/KI); 21 (R26AID+/KIp48CRE+/KI). **P = 0.0098."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "CD3 immunohistochemistry of pancreas from 75-week-old R26AID+/+p48CRE+/KI and R26AID+/KIp48CRE+/KI mice. Left: Representative image of a CD3 infiltrate in a R26AID+/KIp48CRE+/KI mouse. Scale bar: 100 μm. Right: Quantification of the number of CD3-positive cells per mm2 of tissue. n = 12 (R26AID+/+p48CRE+/KI); 13 (R26AID+/KIp48CRE+/KI). *P = 0.0149."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "Representative immunofluorescence staining of CD3 and CD8 in pancreas from 20-week-old R26AID+/+p48CRE+/KI and R26AID+/KIp48CRE+/KI mice. Scale bar: 20 μm."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "TNF-α expression. Total RNA was isolated from pancreas of aged (75-week-old) R26AID+/KIp48CRE+/KI and control mice, and TNF-α expression was quantified by qRT-PCR. Each dot represents an individual mouse. n = 8 (R26AID+/+p48CRE+/KI); 15 (R26AID+/KIp48CRE+/KI). *P = 0.0392."
NCBI gene IDs:
"AID: 11628
p48: 16391
TNF-α: 21926"
Figure legend: "Cell death detection. Pancreas from 75-week-old R26AID+/+p48CRE+/KI and R26AID+/KIp48CRE+/KI mice were stained with anti-caspase-3. Left: Representative staining from an R26AID+/+p48CRE+/KI mouse (top) and an R26AID+/KIp48CRE+/KI mouse (bottom). Scale bar: 50 μm. Right: Quantification of number of cells per mm2 of tissue. Each dot represents an individual mouse. n = 9 (R26AID+/+p48CRE+/KI); 11 (R26AID+/KIp48CRE+/KI). P = 0.0545."
NCBI gene IDs:
"AID: 11628
p48: 16391"
Figure legend: "(J and Anti-GFP  signals are grossly similar in 3-6h and 4-6d UAS-myrGFP;  fruGAL4 males (J), n = 6 for each. Data information: Scale bars, 100 μm."
NCBI gene IDs:
"GFP: 
fru: 42226
GAL4: 855828"
Figure legend: "(A) Wing extension indices  (zero) of isolated 3-6h juvenile w1118/Y; UAS-dTrpA1/+; fruGAL4/+, Met27/Y; UAS-dTrpA1/+; fruGAL4/+ and Gce2.5k/Y; UAS-dTrpA1/+; fruGAL4/+ males at 22 and 27℃. n = 20 for each. (B and C) Wing extension indices (B) and latencies   (C) of isolated 4-6d adult w1118/Y; UAS-dTrpA1/+; fruGAL4/+, Met27/Y; UAS-dTrpA1/+; fruGAL4/+ and Gce2.5k/Y; UAS-dTrpA1/+;  fruGAL4/+ males at 22 and 27℃. n = 20 for each. n.s., not significant, *p &lt; 0.05, ***p &lt; 0.001, Mann-Whitney U test for (B) and unpaired t test for (C). NA, not applicable for latency  analysis. Data information: Error bars indicate SEM."
NCBI gene IDs:
"fru: 42226
GAL4: 855828
Gce: 32457
Met: 32114
TrpA1: 39015"
Figure legend: "(E and F) Tracking indices of 3-6h and 4-6d w1118/Y; UAS-dTrpA1/+; fruGAL4/+, Met27/Y; UAS-dTrpA1/+; fruGAL4/+ and Gce2.5k/Y; UAS-dTrpA1/+; fruGAL4/+ males toward moving dots at 22 and 27℃. n = 24 for each in (E). n = 22, 20, 24, 24, 24 and 24 from left to right in (F). n.s., not significant, ***p &lt; 0.001, Mann-Whitney U test. Data information: Error bars indicate SEM."
NCBI gene IDs:
"fru: 42226
GAL4: 855828
Gce: 32457
Met: 32114
TrpA1: 39015"
Figure legend: "(A and B) qPCR  experiments show that Met  (A) and Gce  (B) mRNA levels are significantly knocked down in actin-GAL4&gt;UAS-Met::Gce-IR adult flies. n = 9 for each. ***p&lt;0.001, unpaired t test. Data information: Error bars indicate SEM."
NCBI gene IDs:
"actin: 31521
GAL4: 855828
Gce: 32457
Met: 32114"
Figure legend: "Courtship behaviors of 3-6h juvenile  and 4-6d adult  males are not significantly affected by knocking down JH receptors Met  and Gce  (UAS-Met::Gce-IR) pan-neuronally  or specifically in neurons  driven by 57C10-GAL4  (C), fruGAL4 (D) N = 22 for each in (C), n = 24 for each in (D) n.s., not significant, Mann-Whitney U test. Data information: Error bars indicate SEM."
NCBI gene IDs:
"fru: 42226
GAL4: 855828
Gce: 32457
Met: 32114
57C10: 38196"
Figure legend: "Courtship behaviors of 3-6h juvenile  and 4-6d adult  males are not significantly affected by knocking down JH receptors Met  and Gce  (UAS-Met::Gce-IR) pan-neuronally  or specifically in R41A01-GAL4  (E), NPF-GAL4  (F), TH-GAL4  (G) and R71G01-GAL4  (H) respectively. n = 24, 24, 21, and 22 from left to right in (E), n = 24, 24, 24, and 23 from left to right in (F), n = 24, 24, 20 and 24 from left to right in (G) and n = 24, 24, 23 and 24 from left to right in (H). n.s., not significant, Mann-Whitney U test. Data information: Error bars indicate SEM."
NCBI gene IDs:
"R41A01: 40940
GAL4: 855828
Gce: 32457
Met: 32114
NPF: 42018
TH: 38746
R71G01: 31469"
Figure legend: "(A-D) Expression  pattern of R41A01-GAL4  (pCd, A), NPF-GAL4  (B), TH-GAL4  (C), and R71G01-GAL4  (P1, D) in adult male brains. Representative of three samples for each. Data information: Scale bars, 100 μm."
NCBI gene IDs:
"pCd: 40940
R41A01: 40940
GAL4: 855828
NPF: 42018
TH: 38746
P1: 31469
R71G01: 31469"
Figure legend: "(I-L) Courtship behaviors of 4-6d adult males  toward virgin females  are similarly intensive and not significantly influenced by activating pCd  (I), NPF  (J), TH  (K) or P1  (L) neurons  at 29℃. n = 24 for each in (I), n = 24, 24, 23, and 23 from left to right in (J), n = 24, 24, 24, and 23 from left to right in (K), and n = 24 for each in (L). n.s., not significant, **p &lt; 0.01, ***p &lt; 0.001, Mann-Whitney U test. Data information: Error bars indicate SEM."
NCBI gene IDs:
"pCd: 40940
NPF: 42018
TH: 38746
P1: 31469"
Figure legend: "(A and B) Anti-GFP  signals are grossly similar in 3-6h and 4-6d UAS-myrGFP/+; dsxGAL4/+ males (A), which are quantified in (B). n = 6 and 8, respectively. n.s., not significant, Mann-Whitney U test. Data information: Scale bars, 50 μm. Error bars indicate SEM."
NCBI gene IDs:
"GFP: 
dsx: 40940
GAL4: 855828"
Figure legend: "(E-L) Synaptic  transmission levels are visualized by syb-GRASP   signals (GFP). (E and F) syb-GRASP  signals from NPF neurons  to dsx neurons  are only observed in 4-6d adult  males but not in 3-6h or 1d males (E), which are quantified in (F). The genotype is LexAop-nSyb-spGFP1-10, UAS-CD4-spGFP11/+; NPF-lexA/dsxGal4. n = 12, 7 and 5 from left to right. (G and H) syb-GRASP  signals from dsx neurons   to NPF neurons  in 4-6d adult  males are much stronger than those in 3-6h and 1d males. syb-GRASP  signals in (G) are quantified in (H). The genotype is UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11/+; NPF-lexA/dsxGal4. n = 13, 7 and 6 from left to right. (I and J) syb-GRASP  signals from TH neurons  to dsx neurons  increase with age  from 3-6h juvenile  males to 4-6d adult  males (I), which are quantified in (J). The genotype is LexAop-nSyb-spGFP1-10, UAS-CD4-spGFP11/TH-LexA; dsxGal4/+. n = 12, 5 and 6, respectively. (K and L) syb-GRASP  signals from dsx neurons  to TH neurons  gradually increase from juvenile  males to adult  males. syb-GRASP  signals in (K) are quantified in (L). The genotype is UAS-nSyb-spGFP1-10, LexAop-CD4-spGFP11/TH-LexA; dsxGal4/+. n = 14, 6 and 6, respectively. White arrowheads indicate syb-GRASP  signals in the same position in brains  of juvenile  males and adult  males. n.s., not significant, **p &lt; 0.01, ***p &lt; 0.001, Mann-Whitney U test. Data information: Scale bars, 50 μm. Error bars indicate SEM."
NCBI gene IDs:
"GFP: 
CD4: 920
dsx: 40940
Gal4: 855828
lexA: 948544
LexA: 948544
NPF: 42018
nSyb: 38196
TH: 38746"
Figure legend: "(A-D) Activation of NPF neurons  with dTrpA1 induced Ca2+ rise in pC1 dsx neurons  in 4-6d males but not in 3-6h males. (A and B) Ca2+  imaging of pC1 neurons  from 22˚C to 27˚C and 30˚C in 3-6h (A) and 4-6d (B) males. (C) Time-course of fluorescent changes in pC1 neurons  upon activation of NPF neurons  in 3-6h and 4-6d males (UAS-GCaMP6m/LexAop-dTrpA1; NPF-lexA/dsxGAL4), and in 4-6d control males (UAS-GCaMP6m/+; dsxGAL4/+) without NPF neuronal  activation, which are quantified in (D). n = 8 for 4-6d control males, 15 for 3-6h and 8 for 4-6d males with dTrpA1. n.s., not significant, *p &lt; 0.05, **p &lt; 0.01, ***p&lt;0.001, unpaired t test. Data information: Scale bars, 10 μm. Error bars indicate SEM."
NCBI gene IDs:
"dsx: 40940
GAL4: 855828
LexA: 948544
lexA: 948544
NPF: 42018
TrpA1: 39015"
Figure legend: "(E-H) Activation of TH neurons  with dTrpA1 triggered  Ca2+ increase in pC1 dsx neurons  in both 3-6h and 4-6d males. (E and F) Ca2+ imaging of pC1 neurons  from 22˚C to 27˚C and 30˚C in 3-6h (E) and 4-6d (F) males. (G) Time-course of fluorescent changes in pC1 neurons  upon activation of TH neurons  in 3-6h and 4-6d males (UAS-dTrpA1/LexAop-GCaMP6s; dsxLexA/TH-GAL4), and in 4-6d control males (LexAop-GCaMP6s/+; dsxLexA/+) without activating TH neurons, which are quantified in (H). n = 8 for each. n.s., not significant, *p &lt; 0.05, Mann-Whitney U test. Data information: Scale bars, 10 μm. Error bars indicate SEM."
NCBI gene IDs:
"dsx: 40940
GAL4: 855828
LexA: 948544
TH: 38746
TrpA1: 39015"
Figure legend: "A. Results of CRISPR screening analyzed by the MAGeCK algorithm."
NCBI gene IDs:
"CRISPR: "
Figure legend: "B. Wild type (WT) and NCOA4 KO MEFs were pretreated with 25 μg/ml ferric ammonium citrate (FAC) for 16 h and then chased with 20 μg/ml cycloheximide (CHX) for the indicated times. Soluble ferritin expression levels were determined by immunoblotting with the indicated antibodies."
NCBI gene IDs:
"NCOA4: 27057"