EMBO/SourceData huggingface dataset:
Figure legend: "(C) End fusion analysis for domain deletion mutants of Rap1. NotI-digested genomic DNA  from nitrogen-starved cells was analyzed by pulsed-field gel electrophoresis  (PFGE) and Southern blot with probes specific for the terminal C, I, L, and M fragments from  chromosomes  I  and II."
NCBI gene IDs:
"Rap1: 2540115"
Figure legend: "(D) PFGE  analysis as in (C) for nitrogen-starved cells harboring N-terminal truncation mutants of Rap1. All mutants were V5-tagged at their C-terminus."
NCBI gene IDs:
"V5: 
Rap1: 2540115"
Figure legend: "(A)  Telomere   length analysis of cells from different sequential restreaks following introduction of Poz1-V5-Rap1_491-693 into rap1∆ cells. Telomere  length was assessed by Southern blotting  of EcoRI-digested genomic DNA  probed with a telomere  specific probe. (B) PFGE  analysis of nitrogen-starved Poz1-V5-Rap1_491-693 cells after 5 and 14 restreaks."
NCBI gene IDs:
"V5: 
Poz1: 2542244
rap1: 2540115
Rap1: 2540115"
Figure legend: "(C) Telomere   length analysis of cells with  Rap1∆RCT fused to Taz1  or Poz1  with intervening V5  tag. The fusion protein was integrated at the endogenous rap1  locus in the context of taz1∆ or poz1∆, respectively. Different sequential restreaks following introduction of the fusion construct were analyzed. (D) PFGE  analysis for nitrogen-starved Rap1∆RCT-V5-Taz1  and Rap1∆RCT-V5-Poz1  from 2nd restreak."
NCBI gene IDs:
"V5: 
Poz1: 2542244
poz1: 2542244
rap1: 2540115
Rap1: 2540115
Taz1: 2542313
taz1: 2542313"
Figure legend: "(B) PFGE  analysis of cells containing plasmids of  Rap1_440-693 with mutations of the amino acids identified in (A) in the context of rap1∆. Cells were subjected to nitrogen-starvation after recovery from transformation (Restreak 0). (C) PFGE  analysis of nitrogen-starved cells harboring the same mutations after 5 restreaks on plates (approximately 110 generations). (D) Quantification of (B) and (C) using Fiji. The y-axis shows the percentage of intensity represented by the three fusion bands (I+L, I+M and L+M) relative to the total intensity of the fused and unfused signals. WT: wildtype  Rap1_440-693."
NCBI gene IDs:
"Rap1: 2540115
rap1: 2540115"
Figure legend: "(B) Western blot  analysis of N-terminally V5-tagged truncation mutants using anti-V5  antibody. Anti-α-tubulin was used as loading control. Quantification of Rap1  mutant protein levels relative to WT normalized to α-tubulin is shown beneath the gel."
NCBI gene IDs:
"V5: "
Figure legend: "(C) Telomere   length analysis  of the mutants by Southern blot  with telomere  specific probe. All mutants are V5-tagged except for ∆491-638 untagged (lane 7)."
NCBI gene IDs:
"V5: "
Figure legend: "(D) PFGE  analysis of the same strains in (C) that were nitrogen-starved. (E) PFGE  analysis of nitrogen-starved internal deletion mutants in poz1∆ background."
NCBI gene IDs:
"poz1: 2542244"
Figure legend: "(A) PFGE  analysis of nitrogen-starved N-terminally V5-tagged full length Rap1  mutants. All mutants were integrated at the endogenous rap1  locus. M5A is the mutation of all 5 non-alanine amino acids under selection in the p-patch to alanine. (B) PFGE  analysis of nitrogen-starved N-terminally V5-tagged rap1_440-693 mutants integrated at the endogenous rap1  locus."
NCBI gene IDs:
"V5: 
rap1: 2540115
Rap1: 2540115"
Figure legend: "(C) PFGE  analysis of nitrogen-starved rap1  mutants in poz1∆ background."
NCBI gene IDs:
"poz1: 2542244
rap1: 2540115"
Figure legend: "(D) PFGE  analysis of nitrogen-starved rap1  mutants in  ppm1∆ background. Untagged Rap1   are in lanes 1-2 and N-terminally V5-tagged in lanes 3-7."
NCBI gene IDs:
"V5: 
rap1: 2540115
Rap1: 2540115
ppm1: 2541346"
Figure legend: "(A) ChIP-qPCR  analysis of N-terminally V5-tagged WT, F545L and M6 mutants of Rap1. Untagged Rap1   was used as a control. Bars represent mean of percentage of DNA   precipitated with anti-V5  antibody conjugated beads relative to input DNA. Error bars represent SEM. N= 4 biological replicates. Two-tailed unpaired Student's t-test was performed and significance is indicated. ***: p &lt; 0.001, **: 0.001 ≤ p &lt; 0.01, n.s.: p&gt;0.05. (B) ChIP-qPCR  analysis of N-terminally V5-tagged WT, F545L and M6 mutants of Rap1  in poz1∆ background performed as in (A). N= 4 biological replicates. Statistical analysis is the same as (A)."
NCBI gene IDs:
"V5: 
poz1: 2542244
Rap1: 2540115"
Figure legend: "(C) Single-stranded overhang measured by denaturing gel electrophoresis of duplex-specific nuclease  digested genomic DNA  and Southern blot with oligonucleotide probes specific for the G-strand (top panel) and  C-strand (bottom panel). WT, F545L and M6 mutants of Rap1  in the presence or absence of poz1  or ppm1  were analyzed. A 21-mer of (GGTTACA)3 was used as a positive control and size marker for  G-strand and a (TGTAACC)3 oligo was used for the  C-strand."
NCBI gene IDs:
"poz1: 2542244
Rap1: 2540115
ppm1: 2541346"
Figure legend: "(B) Stacking bar chart showing telomere  distribution (labeled by Pot1-GFP) in each of the three zones. 100 telomere  foci were quantified per sample. Chi square tests were performed by comparing the rap1  mutants to WT or bqt4∆. Significance is indicated. ***: p &lt; 0.001."
NCBI gene IDs:
"bqt4: 2540694
rap1: 2540115"
Figure legend: "(C) Yeast two hybrid assay  probing interaction  between Rap1  and Bqt4. WT and mutants of Rap1  were fused to the DNA-binding domain (BD) in pGBKT7. Bqt4   and Taz1   were fused to the Gal4  activation domain (AD) in pGADT7. Cells (5μl) carrying both plasmids at 5 x106 cells/ml were spotted onto -Leu-Trp dropout plates and -Leu-Trp-Ade-His  dropout plates to select for ADE2  and HIS3  markers. \"-\": Empty BD or AD plasmid."
NCBI gene IDs:
"Bqt4: 2540694
Gal4: 855828
Rap1: 2540115
Taz1: 2542313"
Figure legend: "(D) PFGE  analysis of nitrogen-starved bqt4∆ and bqt3∆ cells in the presence or absence of poz1."
NCBI gene IDs:
"bqt3: 2539582
bqt4: 2540694
poz1: 2542244"
Figure legend: "(E) End fusions in bqt4∆poz1∆ and bqt3∆poz1∆ are dependent on the presence of ligase IV. PFGE  analysis of nitrogen-starved cells in the presence or absence of lig4."
NCBI gene IDs:
"bqt3: 2539582
bqt4: 2540694
lig4: 2539042
ligase IV: 2539042
poz1: 2542244"
Figure legend: "B Representative western blots (upper) and analysis (lower) of the expression levels of Syb2 in control (Ctrl) and Syb2-cKO DRG neurons (n = 3 per group). Control DRG neurons is from homozygous floxed Syb2-null mice infected with AAV5-CAG-EGFP virus."
NCBI gene IDs:
"EGFP: 
Syb2: 22318"
Figure legend: "C Evoked EPSCs and statistics from DH neurons co-cultured with control (Ctrl) or Syb2-cKO DRG cells (cKO) in 0Ca2+ solution (n = 13 cells for Ctrl and 11 for cKO)."
NCBI gene IDs:
"Syb2: 22318"
Figure legend: "F Left panels, evoked EPSCs from DH neurons co-cultured with scrambled shRNA control (Ctrl) or Cav2.2-KD (sh-1/sh-2) DRG neurons in 0Ca2+ solution. Right panel, quantification of evoked EPSCs (n = 17 cells for Ctrl, 12 for sh-1, and 19 for sh-2). EPSCs were evoked by local electrical stimulation (Estim, at arrows)."
NCBI gene IDs:
"Cav2.2: 257648"
Figure legend: "(A) BACE1 internalization kinetics after 20 minutes (WT: 19.79±1.89%, KO: 13.28±1.57%, p WT vs KO=0.017) and 40 minutes of HA antibody chase (WT: 15.99±2.04, KO: 9.29±1.33, pWT vs KO=0.010) is significantly decreased in AP-2µ KO neurons (27 WT and 29 KO neurons). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(B) Representative examples of internalized BACE1 after 5 min of HA antibody pulse in HA-BACE1-eGFP-overexpressing WT and KO neurons. Scale bar: 5µm."
NCBI gene IDs:
"BACE1: 
eGFP: 
HA: "
Figure legend: "(C, D) Internalized BACE1 levels after 20 min of HA antibody pulse in HA-BACE1-eGFP-overexpressing WT and KO neurons, calculated as the HA (internalized)/eGFP (total) signal intensity ratio (WT: 0.50±0.052, KO: 0.23±0.036, p=0.000, 30 WT and 36 KO neurons, N=4 biological replicates). Scale bar: 5 µm. Data information: Experimental designs are depicted in the top panels where analyzed BACE1 fraction is marked with the red square. All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
HA: 
BACE1: 23621"
Figure legend: "(E, F) Recycled BACE1 levels after 20 min of HA antibody pulse in HA-BACE1-eGFP-overexpressing WT and KO neurons, calculated as the HA(recycled)/eGFP(total) signal intensity ratio (WT: 0.29±0.031, KO: 0.53±0.040, p&lt;000, 30 WT and 32 KO neurons, N=4 biological replicates). Scale bar: 5 µm. Data information: Experimental designs are depicted in the top panels where analyzed BACE1 fraction is marked with the red square. All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
HA: 
BACE1: 23621"
Figure legend: "Recycled levels of BACE1 after 20 min of HA antibody pulse in HA-BACE1-mCherry-transfected WT and KO neurons, co-expressing either eGFP-RAB4 or eGFP-RAB4-S22N pWTRAB4 vs pWTRAB4S22N&gt;0.999, 34-38 neurons, N=4 biological replicates). Scale bar: 5 µm. Data information: Experimental designs are depicted in the top panels , where analyzed BACE1 fraction is marked with the red square."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
BACE1: 23621
RAB4: 5867"
Figure legend: "Recycled levels of BACE1 after 20 min of HA antibody pulse in HA-BACE1-mCherry-transfected WT and KO neurons, co-expressing either eGFP-RAB4 or eGFP-RAB4-S22N, calculated as the HA(recycled)/mCherry(total) signal intensity ratio (WTRAB4: 0.14±0.02, KORAB4: 0.20±0.02, WTRAB4-S22N: 0.14±0.02, KORAB4S22N:0.14±0.01, pWTRAB4 vs pKORAB4=0.033, pKORAB4 vs pKORAB4S22N=0.035, pWTRABS22N vs pKORABS22N=0.999, pWTRAB4 vs pWTRAB4S22N&gt;0.999, 34-38 neurons, N=4 biological replicates). Scale bar: 5 µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA in n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
RAB: 
BACE1: 23621
RAB4: 5867"
Figure legend: "Recycled levels of BACE1 after 20 min of HA antibody chase in HA-BACE1-mCherry-transfected WT and KO neurons, co-expressing either eGFP-RAB11 or eGFP-RAB11-S22N, calculated as the HA(recycled)/mCherry(total) signal intensity ratio (WTRAB11: 0.11±0.01, KORAB11: 0.29±0.02, WTRAB11A-S25N: 0.14±0.02, KORAB11A-S25N: 0.19±0.02, pWTRAB11 vs pKORAB11&lt;0.0001, pWTRAB11 vs pWTRAB11A-S25N=0.761, pKORAB11 vs pKORAB11A-S25N=0.001, pWTRAB11A-S25N vs pKORAB11A-S25N=0.175, 28-37 neurons per condition, N=3 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
BACE1: 23621
RAB11: 8766
RAB11A: 8766"
Figure legend: "Internalized levels of BACE1 after 20 minutes of HA antibody uptake in HA-BACE1-mCherry transfected WT and KO neurons, co-expressing either eGFP-RAB4 or eGFP-RAB4-S22N pWTRAB4 vs pWTRAB4S22N=0.450, 27-37 neurons per condition, N=3 biological replicates). Scale bar: 5 µm. Data information: Experimental designs are depicted in the top panels where analyzed BACE1 fraction is marked with the red square."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
BACE1: 23621
RAB4: 5867"
Figure legend: "Internalized levels of BACE1 after 20 minutes of HA antibody uptake in HA-BACE1-mCherry transfected WT and KO neurons, co-expressing either eGFP-RAB4 or eGFP-RAB4-S22N, calculated as the HA(internalized)/mCherry(total) signal intensity ratio (WTRAB4: 0.54±0.05, KORAB4: 0.33±0.02, WTRAB4-S22N: 0.63±0.06, KORAB11A-S25N: 0.50±0.04, pWTRAB4 vs pKORAB4=0.004, pKORAB4 vs pKORAB4S22N=0.046, pWTRABS22N vs pKORABS22N=0.182, pWTRAB4 vs pWTRAB4S22N=0.450, 27-37 neurons per condition, N=3 biological replicates). Scale bar: 5 µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by ANOVA n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
RAB: 
BACE1: 23621
RAB4: 5867"
Figure legend: "(L, M) BACE1 surface levels are increased in AP-2µ KO neurons, when compared to the WT set to 100% (KO: 129.65±5.30%, p=0.005, N=4 biological replicates). (N) Surface versus total BACE1 ratio is not altered in AP-2µ KO neurons when normalized to the WT set to 100% (KO: 83.02±10.93%, p=0.109, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test in (M,N). n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(A, B) BACE1 protein levels in cortical lysates of WT and AP-2µ KO mice. Protein levels in the KO were normalized to the WT set to 100% (KO: 447.64±48.78%, p&lt;0.000, N=7 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(C, D) Immunofluorescence levels of BACE1 are significantly increased in the cortex of AP-2µ KO mice when normalized to the WT set to 100% (KO: 162.714±11.082%, p=0.005, N=4, biological replicates). Scale bar: 100µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(E) Localization of BACE1 in cultured WT and AP-2µ KO neurons. Scale bars: 20 µm, 5 µm (inserts)."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(K, L) Colocalization of BACE1 and RAB7A in cortical sections of WT and AP-2μ ΚΟ brains, quantified using Pearson's colocalization coefficient (WT: 0.18±0.04, KO: 0.35±0.05, p=0.038, N=4 biological replicates). Scale bar: 10 µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2μ: 11773"
Figure legend: "(M Representative fluorescence images of WT neurons transfected with HA-BACE1-mCherry and immunostained for Cathepsin D. Channels were false color-coded to better illustrate the colocalization. Scale bars: 10µm. Arrowheads indicate BACE1 accumulation in lysosomes in the WT Dotted line outlines the neuron."
NCBI gene IDs:
"HA: 
mCherry: 
BACE1: 23621"
Figure legend: "N) Representative fluorescence images of AP-2µ KO neurons transfected with HA-BACE1-mCherry and immunostained for Cathepsin D. Channels were false color-coded to better illustrate the colocalization. Scale bars: 10µm. Arrowheads indicate BACE1 absence in the KO. Dotted line outlines the neuron."
NCBI gene IDs:
"HA: 
mCherry: 
AP-2µ: 11773
BACE1: 23621"
Figure legend: "(Q, R) Representative fluorescence images of WT and AP-2μ KO neurons transfected with BACE1-mKeima-Red and co-transfected with eBFP to reveal the neuronal morphology. Scale bars: 10µm, 2µm in zoomed images."
NCBI gene IDs:
"eBFP: 
mKeima: 
AP-2μ: 11773
BACE1: 23621"
Figure legend: "(A, B) Representative fluorescence images (A) and corresponding kymographs (B) from time-lapse videos of control neurons transfected with HA-BACE1-eGFP and co-transfected with AP-2μ-mCherry. Scale bars: (A) 2μm, 1.6µm inserts, (B) x=2μm, y=5s. Arrowheads in (A) correspond to BACE1 carriers indicated by the same arrowheads in (B)."
NCBI gene IDs:
"eGFP: 
HA: 
mCherry: 
AP-2μ: 116563
BACE1: 23621"
Figure legend: "(C) Representative fluorescence images and corresponding kymographs from time-lapse videos of WT and AP-2μ KO neurons transfected with HA-BACE1-eGFP. Scale bar: 5µm top panels, x=2μm, y=5s bottom panels."
NCBI gene IDs:
"eGFP: 
HA: 
AP-2μ: 11773
BACE1: 23621"
Figure legend: "(D) Loss of AP-2μ significantly decreases the BACE1 motility (WT: 37.42±3.79%, KO: 23.07±3.07%, p=0.003, WT=31, KO=32 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2μ: 11773"
Figure legend: "(E) Loss of AP-2μ significantly decreases the anterograde and retrograde BACE1 velocity (WTAntero: 0.37±0.02 μm/s, KOAntero: 0.22±0.03 μm/s, pWTAntero vs pKOAntero&lt;0.000; WTRetro: 0.35±0.02 μm/s, KORetro: 0.25±.03 μm/s, pWTRetro vs pKORetro =0.006, WT=35 and KO=32 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2μ: 11773"
Figure legend: "(F) Representative fluorescence images and corresponding kymographs from time-lapse videos of WT and AP-2μ KO neurons co-transfected with HA-BACE1-GFP and AP-2μ-mRFP. Scale bar: 5µm top panels, x=2μm, y=5s bottom panels."
NCBI gene IDs:
"GFP: 
HA: 
mRFP: 
AP-2μ: 11773
AP-2μ: 116563
BACE1: 23621"
Figure legend: "(G) Bi-directional mobility of BACE1 carriers in WT and AP-2μ ΚΟ neurons, co-expressing the AP-2μ-mRFP (WT: 37.60±2.20%, KO: 25.61±2.67%, WTAP-2μ-mRFP: 45.04±3.27%, KOAP-2μ-mRFP: 40.07±2.37%, pWT vs KO=0.005, pKO vs KO+AP-2μ-mRFP&lt;0.000, pWT vs WT+AP-2μ-mRFP=0.186, pWT+AP-2μ-mRFP vs KO+AP-2μ-mRFP=0.570, 43-53 neurons per condition, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by two-way ANOVA in (G). n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"mRFP: 
AP-2μ: 11773
AP-2μ: 116563"
Figure legend: "(H) Representative kymographs of WT and AP-2μ KO neurons co-expressing the HA-BACE1-GFP and tagRFP-LC3B. Scale bars: x=2μm, y=5s."
NCBI gene IDs:
"GFP: 
HA: 
RFP: 
AP-2μ: 11773
BACE1: 23621
LC3B: 64862"
Figure legend: "(I) Reduced bi-directional mobility of BACE1-containing autophagosomes in AP-2µ KO neurons. (WT: 33.53±4.19%, KO: 19.45±5.14%, p=0.000, WT=37 and KO=35 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(J) Representative kymographs of WT neurons co-expressing HA-BACE1-GFP and AP2-αΑ WT or AP2-αΑ Μut. Scale bar: 5µm top panels, x=2μm, y=5s bottom panels."
NCBI gene IDs:
"GFP: 
HA: 
BACE1: 23621
AP2-α: 21418"
Figure legend: "(K) Mobility of BACE1 carriers is significantly decreased in neurons overexpressing the AP2-αΑ Mut compared to neurons expressing the AP2-αΑ WT (WT: 52.23±4.16%, Mut: 27.95±3.31, p=&lt;0.000, WT=26 and Mut=29 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP2-α: 21418"
Figure legend: "(L) Representative fluorescence images and corresponding kymographs from time-lapse videos of WT neurons transfected with either HA-BACE1-GFP or HA-BACE1-LL/AA-GFP. Scale bar: 5µm top panels, x=2μm, y=5s bottom panels."
NCBI gene IDs:
"GFP: 
GFP.: 
HA: 
BACE1: 23621"
Figure legend: "(M) Reduced mobility of BACE1-LL/AA-positive vesicles, when compared to WT BACE1 (WT: 38.97±1.40%, LL/AA: 19.89±1.77%, p&lt;0.000, WT=31 and LL/AA=33 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"BACE1: 23621"
Figure legend: "(N) Reduced velocity of BACE1-LL/AA-positive vesicles, when compared to WT BACE1 (WTAntero: 0.38±0.03 μm/s, LL/AAAntero: 0.15±0.03 μm/s, pWTAntero vs pLL/AAAntero&lt;0.000; WTRetro: 0.39±0.03 μm/s, LL/AARetro: 0.25±.03 μm/s, pWTRetro vs pLL/AARetro =0.000, WT=31 and LL/AA=33 neurons, N=4 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"BACE1: 23621"
Figure legend: "(A, B) The eGFP/mCherry signal intensity ratio is significantly increased in mCherry-APP-P1-eGFP-expressing KO neurons comparing to the WT (WT: 0.94±0.06%, KO: 1.65±0.18, p=0.000, WT=39 and KO=41 neurons, N=5 biological replicates). Scale bars: 2μm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"eGFP: 
mCherry: 
APP: 351"
Figure legend: "Confocal images of WT and AP-2μ ΚΟ entorhinal cortex immunostained with antibody recognizing total APP and APP-CTFs. Scale bars: 100 µm (C)"
NCBI gene IDs:
"AP-2μ: 11773"
Figure legend: "Confocal images of WT and AP-2μ ΚΟ entorhinal cortex immunostained with antibody recognizing total APP and APP-CTFs. Scale bars: 20µm (D)."
NCBI gene IDs:
"AP-2μ: 11773"
Figure legend: "(E-G) Levels of APP-CTFs are significantly increased in AP-2µ KO cortex compared to the WT set to 100% (KOC99: 156.65±8.37%, p=0.004, N=4 biological replicates; KOC83: 154.79±15.6%, p=0.012; N=5 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(H) Levels of full-length (FL) APP are unaltered in AP-2µ KO cortex (KOAPP FL: 106.64±12.96%, p=0.315, N=6 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by one-sample Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(I) Levels of Nicastrin (NCT) are unaltered in the AP-2µ KO cortex"
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(J, K) Intracellular levels of Aβ1-40 are significantly increased in AP-2µ KO axons compared to the WT (WTAβ1-40: 1.82±0.12, KOAβ1-40: 2.45±0.12, p=0.000, WT=24 and KO=24 neurons, N=3 biological replicates). Scale bars, 5µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "Intracellular levels of Aβ1-42 are significantly increased in AP-2µ KO axons (WTAβ1-42: 0.65±0.08, KOAβ1-42: 0.91±0.10, p=0.046, WT=38 and KO=38 neurons, N=3 biological replicates). Scale bar: 5 µm."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "Intracellular levels of Aβ1-42 are significantly increased in AP-2µ KO axons (WTAβ1-42: 0.65±0.08, KOAβ1-42: 0.91±0.10, p=0.046, WT=38 and KO=38 neurons, N=3 biological replicates). Scale bar: 5 µm. Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2µ: 11773"
Figure legend: "(N) Significantly increased ratio of Αβ1-42/ Αβ1-40 measured by ELISA in the media of cultured AP-2μ KO neurons compared to the WT set to 100% (KO:124.57±10.97%, p=0.037, N=6 biological replicates). Data information: All graphs show mean ± SEM, statistical analysis was performed by unpaired two-tailed Student's t‐test n.s.-non-significant. * indicates p≤0.05, ** indicates p≤0.01, *** indicates p≤0.001."
NCBI gene IDs:
"AP-2μ: 11773"