EMBO/SourceData huggingface dataset:
Figure legend: "G, H. Confocal images of coronal sections from E14 brains of wild type mice stained with anti-JAM-A antibody (green), phalloidin to label actin (gray) and DAPI to label cell nuclei (blue). Scale bars: 10 μm (G), 5 μm (H).I. Quantification of JAM-A protein expression in the ventricular zone of E14 brain coronal sections from miR-34/449 KO mice compared with littermate controls (Het) by immunofluorescence. ACTIN (phalloidin signal) was used to normalize (norm.) the expresion levels of JAM-A. p = 0.04842 (n = 5 (KO) or 7 (het) cortices per genotype group, 3 independent litters)."
NCBI gene IDs:
"miR-34: 723932///723849///723848"
Figure legend: "A, D. Confocal time lapse images of metaphase HeLa cells stably expressing a centriole marker (Centrin2-GFP; green), the microtubule marker (α-tubulin-mRFP; red) and no extra protein (A) or mouse JAM-A (D), 48 h after transfection of miR-449a mimic, JAM-A siRNA, or non-targeting negative control siRNA. Scale bar, 10 μm.B, E. Quantification of spindle rotation for cells as shown in A and D. Each dot represents a single cell. Red bar indicates median. Significance was tested by Pairwise t-test with Bonferroni correction. p-value = 2e-11 (Neg. control versus miR449 mimic), p-value = 0.01928 (Neg. control versus JAM-A siRNA), p-value = 0.17492 (Neg. control versus miR449 mimic, under JAM-A overexpression). * indicates p ≤ 0.05; ** p ≤ 0.01; p*** p ≤ 0.001 for all panels.C, F. Cumulative histograms of mitotic duration from nuclear envelope breakdown until anaphase onset, measured for cells as shown in A and D; n ≥ 20 cells for all conditions, 2 independent replicates."
NCBI gene IDs:
"JAM-A: 16456
JAM-A: 50848
miR-449a: 554213"
Figure legend: "Representative images of fluorescence in-situ hybridization of  ADPGK-AS1  (magenta) and nuclei  (blue) in IL-4 stimulated  M2-like macrophages. β-actin served as the cytoplasmic  control and MALAT1   as the nuclear  control. Scale bar = 10 µm."
NCBI gene IDs:
"β-actin: 60
ADPGK-AS1: 100287559
MALAT1: 378938"
Figure legend: "RNA expression  analysis of ADPGK-AS1, 47S ribosomal RNA (nuclear  control), and β-Tubulin (cytoplasmic  control) in IL-4 stimulated M2-like macrophages  after subcellular fractionation. n = 3 independent experiments. Data represent the percentage of total detected RNA."
NCBI gene IDs:
"47S ribosomal RNA: 
ADPGK-AS1: 100287559
β-Tubulin: 203068"
Figure legend: "Subcellular fractionation of IL-4-stimulated M2-like macrophages  and isolation and analysis of ADPGK-AS1  abundance in the cytoplasmic  and mitochondrial  fractions The mitochondrial  encoded RNA mtATP6  served as the mitochondrial  control and β-tubulin as the cytoplasmic  control. n = 3 independent experiments, Data represent the percentage of total detected RNA."
NCBI gene IDs:
"ADPGK-AS1: 100287559
ATP6: 4508
β-tubulin: 203068"
Figure legend: "(A) Validation of the ADPGK-AS1-interacting  protein MRPL35  by RNA immunoprecipitation (RIP) with followed by qPCR  for ADPGK-AS1. n = 4 independent experiments, ***p ≤ 0.001 compared with IgG control."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(B, C) Co-staining for ADPGK-AS1 (magenta) and MRPL35  or MRPL15  (yellow) with respective fluorescence intensity across  co-stained macrophages  (see white line) for  ADPGK-AS1  and MRPL35  or MRPL15. Scale bar = 10 µm (left), 5 µm (right)."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(C, Representative images and quantification of mitochondria  by MitoTracker  (red) in THP1  control and ADPGK-AS1  OE cells. Nuclei  were stained  with DAPI  (blue). Scale bar = 200 µm (left), 100 µm (right, magnification)."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "D) Representative images and quantification of mitochondria  by TOM20  (green) in THP1  control and ADPGK-AS1 OE cells. Nuclei  were stained  with DAPI  (blue). Scale bar = 200 µm (left), 100 µm (right, magnification)."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(E) Representative immunoblot  for mitochondrial protein expression (TOM20, αb-crystallin, PCG-1, MRPL35, cytochrome C) in THP1  control and ADPGK-AS1 OE cells. n = 4 independent experiments."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(G) Gene expression  analysis (fold change) of mitochondrially  encoded genes (ND1, ND2, ND4, ATP6, ATP8, CO1, CO2, CyB) after isolation of mtDNA  from THP1  control and ADPGK-AS1 OE cells. n = 3 independent experiments. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared with control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
ATP6: 4508
ATP8: 4509
CO1: 4512
CO2: 4513
CyB: 4519
ND1: 4535
ND2: 4536
ND4: 4538"
Figure legend: "I) full oxygen  consumption plot (n = 8) by the seahorse assay in THP1  control and ADPGK-AS1  OE cells. **p ≤ 0.01, ***p ≤ 0.001 compared with control."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(K) Representative confocal  fluorescence images and quantification of mitochondrial  shape with the mitochondrial  marker TOM20  (yellow) in THP1  control and ADPGK-AS1  OE cells. Nuclei  were stained  with DAPI  (blue). n = 35 cells, Scale bar=10µm (left), 5µm (magnification, right)."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(L)  Transmission electron microscopy images of mitochondrial  shapes in THP1  control and ADPGK-AS1 OE cells. n = 4 cells, Scale bar = 5 µm (left), 1 µm (right, magnification)."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(B) Expression  analysis of ADPGK-AS1  and MRPL35  (fold change) in THP1  control and  ADPGK-AS1 OE macrophages  transfected with a siRNA against ADPGK-AS1, MRPL35  or negative control. n = 4 independent experiments. ***p ≤ 0.001 compared with ADPGK-AS1  OE siRNA control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
MRPL35: 51318"
Figure legend: "(C) Representative images of JC-1    monomers (green) and aggregates (red) in THP1  control and ADPGK-AS1 overexpressing macrophages  transfected with a siRNA against ADPGK-AS1, MRPL35, or negative control. Scale bar = 100 µm."
NCBI gene IDs:
"ADPGK-AS1: 100287559
MRPL35: 51318"
Figure legend: "(E) Representative images (left panel) and quantification (right panel) of ROS  accumulation in THP1  control and ADPGK-AS1 OE cells transfected with a siRNA against  ADPGK-AS1, MRPL35, or negative control. n = 7. ***p ≤ 0.001 compared to control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
MRPL35: 51318"
Figure legend: "(F) Spare capacity  (oxygen consumption rate) measured with the  seahorse assay in THP1  control and ADPGK-AS1  OE cells transfected with a siRNA against ADPGK-AS1, MRPL35, or negative control. n = 3 independent experiments. ***p ≤ 0.001 compared with control, §p ≤ 0.05, §§§p ≤ 0.001 compared with  ADPGK-AS1 OE siRNA negative control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
MRPL35: 51318"
Figure legend: "(B) mRNA expression  analysis (ΔCT) of macrophage  markers (TNFα, IL-8, CD206, CSF-1R) in co-cultured PBMC-derived macrophages  with tumor cells or macrophage  control (M0). n = 4 biological replicates. *p ≤ 0.05, **p ≤ 0.01 compared with M0."
NCBI gene IDs:
"CSF-1R: 1436
IL-8: 3576
CD206: 4360
TNFα: 7124"
Figure legend: "(C) Expression  of ADPGK-AS1 lncRNA in macrophages  co-cultured with tumor cells (A549) or macrophage  controls (M0). n = 4 biological replicates. *p ≤ 0.05, compared with M0."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(G) Expression  of IDH  mRNA in A549  cells transfected with siRNA specific for IDH  (IDH  KD) or negative control. n = 5 independent experiments. ***p ≤ 0.001 compared with A549  control."
NCBI gene IDs:
"IDH: 3417"
Figure legend: "(H) RNA expression analysis of ADPGK-AS1 in THP1 macrophages  stimulated to have an M1-like  or  M2-like phenotype, untreated (M0) or treated with conditioned medium  (CM) of A549  cells transfected with a siRNA specific for IDH  (IDH  KD) or negative control. n = 5 biological replicates. ***p ≤ 0.001, compared with tumor cell control CM."
NCBI gene IDs:
"ADPGK-AS1: 100287559
IDH: 3417"
Figure legend: "(A, B) mRNA expression  analysis (ΔCT) of M1   (TNFα, IL-8, CXCL10) and M2   (CSF-1R, CD206, IL-10, ALOX15, TGFβ, IL-1Ra) macrophage  marker genes in THP1  control and ADPGK-AS1 OE cells. n = 4 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 compared with control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
ALOX15: 246
CSF-1R: 1436
CXCL10: 3627
IL-8: 3576
IL-10: 3586
IL-1Ra: 3557
CD206: 4360
TGFβ: 7040
TNFα: 7124"
Figure legend: "(E) Migration  (right panel) of A549  cells treated with CM from THP1  control or ADPGK-AS1  OE macrophages. Representative membrane  images are shown in the left panel. n = 5 independent experiments. ***p ≤ 0.001 compared with control. Scale bar = 400 µm."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(G-H) mRNA expression  analysis (ΔCT) of M1   (TNFα, IL-8, CD80, CCR7) and M2   (CSF-1R, CD206, IL-10, TGFβ) macrophage  marker genes in primary M1-like (M1) and   M2-like (M2) macrophages  transfected with antisense LNA GapmeRs specific for ADPGK-AS1  or negative control. n = 5 biological replicates, *p ≤ 0.05, **p ≤ 0.01, compared to M2  control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
CCR7: 1236
CD80: 941
CSF-1R: 1436
IL-8: 3576
IL-10: 3586
CD206: 4360
TGFβ: 7040
TNFα: 7124"
Figure legend: "(K) Migration  (right panel) of tumor cells (A549) treated with CM from PBMC-derived M1-like (M1) or  M2-like  (M2) macrophages  transfected with antisense LNA GapmeRs specific for ADPGK-AS1  or negative control. Representative membrane  images are shown in the left panel. n = 5 biological replicates. *p ≤ 0.05, compared with M2   control. Scale bar = 400 µm."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(C) Representative brightfield  images of tumors (scale bar = 2mm) and fluorescence images (Ki67, cleaved caspase 3, scale bar = 100 µm; cleaved caspase 3 (green) and cytokeratin 18  (magenta), scale bar = 50 µm) and LDH enzyme activity  (scale bar = 25 µm) after co-injection with M1-like (M1) or  M2-like (M2) macrophages  transfected with antisense LNA GapmeRs specific for ADPGK-AS1  or negative control (ctrl) together with A549  tumor cells or A549  cells alone as a control."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(F) RNA expression  analysis of ADPGK-AS1  and MRPL35  in tumor PCLS treated with antisense LNA GapmeRs specific for ADPGK-AS1 or a negative control or seeded with THP1  control or ADPGK-AS1 OE cells. n = 5 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 compared with control GapmeR, §p ≤ 0.05, §§§p ≤ 0.001 compared with EV control."
NCBI gene IDs:
"ADPGK-AS1: 100287559
MRPL35: 51318"
Figure legend: "(G) Multispectral flow cytometry  analysis of  M1-like  and M2-like macrophage populations in tumor PCLS treated with antisense LNA GapmeRs specific for ADPGK-AS1  or a negative control or seeded with THP1  control or ADPGK-AS1  OE cells. n = 6 biological replicates. *p ≤ 0.05 compared with Gapmer negative control, §p ≤ 0.05 compared with EV control."
NCBI gene IDs:
"ADPGK-AS1: 100287559"
Figure legend: "(C) Detection of sAPPα, sNrCAM and mNrCAM in ADAM10 cKO neuronal supernatants and lysates at 7 days in vitro (DIV7). The neurons prepared from floxed ADAM10 (ADAM10 fl/fl) mice were infected with a lentivirus encoding improved Cre recombinase (iCre) or a control GFP lentivirus at DIV2. Conditioned media were collected for 48 h. Densitometric quantifications of the Western Blots are shown (** p &lt; 0.01; *** p &lt; 0.001; **** p &lt; 0.0001, two-sided students t-test n = 6-8)."
NCBI gene IDs:
"GFP: 
ADAM10: 11487
Cre: 2777477"
Figure legend: "HEK 293 cells were transfected with a C-terminally VSV-tagged NrCAM construct or empty vector. Cells were then treated with DAPT (1 µM), GI254023x (5 µM), either substances or solvent for 24 h. The C-terminal NrCAM-fragment was detected with an antibody against the VSV-tag. Representative Western Blots are shown."
NCBI gene IDs:
"VSV: 
NrCAM: 4897"
Figure legend: "(A) ADAM10 fl/fl neurons were treated with an iCre (to induce an ADAM10 KO), or a control lentivirus at DIV2. At DIV7, cell surface proteins were labeled with biotin and enriched by streptavidin pull-down. The biotinylated proteins were detected by immunoblotting. Total lysates were analyzed to compare mNrCAM's surface/lysate levels. Densitometric quantifications of the Western Blots are shown. Two-sided students t-test (**** p &lt; 0.0001, n = 7). Given are mean +/- the standard error of them mean. The mean levels of solvent treated cells were set to 1."
NCBI gene IDs:
"ADAM10: 11487
Cre: 2777477"
Figure legend: "(C) Workflow of the neurite outgrowth assay. (D) Representative pictures of single neurites at time-point 0h (DIV3) and 24h (DIV4). Neurons were infected 4h after plating with lentiviruses encoding GFP (to visualize the neurons) together with shRNA expression cassettes (sh1 and sh2) targeting either NrCAM or carrying a scrambled (scr) control construct. Images of neurites were taken at three days in vitro (DIV3) and 24h later at DIV4. In order to study the effect of ADAM10 on neurite outgrowth, neurons were treated with the ADAM10 inhibitor GI254023x, or vehicle (control), at DIV3, after taking the first pictures with an epifluorescent microscope. The differences in neurite length were calculated as absolute values (neurite length at 24h minus neurite length of 0h) for individual neurites passing through the middle channels of the chambers. Only neurites that had already entered the main channel at 0h and had not yet left those channels at 0h were considered. The red arrows indicate the start and the end of the respective length measurements. The scale bar indicates 40 µm. (E) One-way ANOVA with post hoc Dunnett's test. Given are mean +/- the standard error of them mean (* p &lt; 0.05; ** p &lt; 0.01; *** p &lt; 0.001; **** p &lt; 0.0001, n-numbers for each condition are shown in the graph in 3E)."
NCBI gene IDs:
"GFP: 
NrCAM: 319504"
Figure legend: "HEK293 cells were transiently transfected with hNrCAM, FLAG-tagged hTSPAN15, either plasmids or the empty vector. Detection of sAPPα and sNrCAM in the conditioned media and ADAM10 in the cell lysate. Densitometric quantification of the Western Blots are shown (** p &lt; 0.01), (*** p &lt; 0.001), (**** p &lt; 0.0001), two-sided students t-test, or one-way ANOVA with post hoc Dunnett's test (n = 6). Given are mean +/- the standard error of them mean. The mean levels of solvent treated cells were set to 1. Representative Western Blots are shown."
NCBI gene IDs:
"FLAG: 
NrCAM: 4897
TSPAN15: 23555"
Figure legend: "(G) Percentages of control (n = 210), Mcrs1-KD (n = 182), and Mcrs1-rescue (n = 201) oocytes that underwent GVBD and PBE. The bars are representing the mean ± SEM. The P-values were calculated using Student's t-test. GVBD%: ***P = 0.0003; *P = 0.01, PB1%: *P = 0.03; *P = 0.02."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(A) Representative images of the occurrence of GVBD in control, Mcrs1-KD, and Mcrs1-rescue (Mcrs1 siRNA + Mcrs1 mRNA) oocytes at 3 h following release from IBMX. Scale bar, 100 μm. (B) The incidence of GVBD at 1, 2, and 3 h post-IBMX release in control (n = 155), Mcrs1-KD (n = 157), and Mcrs1-rescue (n = 150) oocytes."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(D) Expression levels of CDK1 in Mcrs1-KD and control oocytes. The bars represent the mean (± SEM) of 5 biological replicates. The P-values were calculated using Student's t-test. *P = 0.01. (E) Expression levels of p-CDK1(Y15) in Mcrs1-KD and control oocytes at 1 h following release from IBMX (200 oocytes per sample). **P = 0.006. (F) Expression levels of cyclin B1 in control and Mcrs1-KD oocytes at 1 h following release from IBMX (200 oocytes per sample). The bars represent the mean (± SEM) of 3 biological replicates. The P-values were calculated using Student's t-test. **P = 0.0042."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(I) The incidence of GVBD at 1, 2, and 3 h post-IBMX release in control oocytes (n = 112), Mcrs1-KD oocytes (n = 98), and Mcrs1-KD oocytes treated with 5 mM WEE1-1N-4 (n = 102). (J) The incidence of GVBD at 1, 2, and 3 h post-IBMX release in control (n = 123), Mcrs1-KD (n = 134), and Mcrs1 siRNA + cyclin B1-GFP (n = 95) oocytes. (K) The incidence of GVBD at 1, 2, and 3 h post-IBMX release in control (n = 92), Mcrs1-KD (n = 97), and Mcrs1 siRNA + cyclin B1-GFP oocytes treated with 5 mM WEE1-1N-4 (n = 106)."
NCBI gene IDs:
"GFP: 
cyclin B1: 268697
Mcrs1: 51812"
Figure legend: "(B) Detection of newly synthesized RNAs by EU incorporation in control, Mcrs1-KD, and Mcrs1-rescue GV oocytes. The scattergram shows the relative fluorescence intensity of EU in control (n = 43), Mcrs1-KD (n = 52), and Mcrs1-rescue (n = 30) GV oocytes. Scale bar, 10 μm. ***P = 0.0001."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(C) Immunofluorescence analysis of H3K4me2 in control and Mcrs1-KD oocytes. Scale bar, 10 μm. Relative fluorescence intensity of H3K4me2 signals in control (n = 26) and Mcrs1-KD (n = 30) GV oocytes. ***P = 0.0001."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(E) Immunofluorescence analysis of H3K9me2 in control and Mcrs1-KD oocytes. Scale bar, 10 μm. Relative fluorescence intensity of H3K9me2 signals in control (n = 29) and Mcrs1-KD (n = 22) GV oocytes. **P = 0.0035. (F) Immunofluorescence analysis of H4K16ac in control and Mcrs1-KD oocytes. Scale bar, 10 μm. Relative fluorescence intensity of H4K16ac signals in control (n = 30) and Mcrs1-KD (n = 28) GV oocytes. *P = 0.022."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(G) Immunofluorescence analysis of HDAC2 in control and Mcrs1-KD oocytes. Scale bar, 10 μm. Relative fluorescence intensity of HDAC2 signals in control (n = 27) and Mcrs1-KD (n = 24) GV oocytes. ***P = 0.0005."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(A) Representative images of spindle assembly at 3 h post-release from IBMX in control and Mcrs1-KD oocytes. Scale bar, 20 μm."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(G) Immunofluorescence analysis of H3S10ph and α-tubulin in control and Mcrs1-KD oocytes. Scale bar, 20 μm. The scattergram shows the relative fluorescence intensity of H3S10ph signals in control (n = 21) and Mcrs1-KD (n = 23) oocytes. *P = 0.017."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(H) Immunofluorescence analysis of γ-tubulin and α-tubulin in control and Mcrs1-KD oocytes. Scale bar, 20 μm. The histogram shows the percentages of control (n = 55) and Mcrs1-KD (n = 63) oocytes with abnormal γ-tubulin. *P = 0.014. (I) Immunofluorescence analysis of acetylated tubulin and α-tubulin in control and Mcrs1-KD oocytes. Scale bar, 20 μm. The scattergram shows the relative fluorescence intensity of acetylated tubulin signals in control (n = 83) and Mcrs1-KD (n = 66) oocytes. **P = 0.0046."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(C) Levels of p-Aurka and p-Aurkc in control and Mcrs1-KD MI oocytes. The blots were probed with anti-p-Aurora A/B/C and anti-actin antibodies. (D) Co-IP was performed with an anti-Mcrs1 antibody. The immunoblots of protein precipitates were probed with an anti-Aurka antibody."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(G) Percentages of control (n = 92), Mcrs1-KD (n = 73), and Aurka-rescue (n = 67) oocytes that underwent PBE. *P = 0.033; *P = 0.018."
NCBI gene IDs:
"Aurka: 20878
Mcrs1: 51812"
Figure legend: "(H) Percentages of control (n = 69), Mcrs1-KD (n = 76), and Aurka-rescue (n = 43) oocytes with abnormal spindles. *P = 0.018; *P = 0.024."
NCBI gene IDs:
"Aurka: 20878
Mcrs1: 51812"
Figure legend: "(K) Immunofluorescence analysis of Kif2A in control and Mcrs1-KD oocytes. Scale bar, 20 μm. The histogram shows the percentages of control (n = 50) and Mcrs1-KD (n = 61) oocytes with abnormal Kif2A. **P = 0.003."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "(L) Immunofluorescence analysis of TPX2 and α-tubulin in control and Mcrs1-KD oocytes. Scale bar, 20 μm. The scattergram shows the relative fluorescence intensity of TPX2 signals in control (n = 48) and Mcrs1-KD (n = 52) oocytes."
NCBI gene IDs:
"Mcrs1: 51812"
Figure legend: "Analysis of long-term multi-lineage HSC potential in E11.5 Sl/Sl AGM+VU. Irradiated CD45.1 syngeneic mice were transplanted with 1 e.e. of wild type, Sl/+ or Sl/Sl CD45.2+ AGM+VU cells. PB chimerism is represented as the percentage of donor CD45.2+ cells among total CD45+ cells, 16 weeks after transplant. A total of 14 recipients were transplanted with wild type or Sl/+ cells and 8 with Sl/Sl cells, over 4 independent experiments. Wild type is represented with a circle, Sl/+ a triangle and Sl/Sl with a square. Tail somite range: 9-17 (+/+, Sl/+); 10-17 (Sl/Sl). *:p=0.027, one-tailed Mann-Whitney's U test; p=0.055, two-tailed Mann-Whitney's U test."
NCBI gene IDs:
"CD45.1: 19264"
Figure legend: "Confocal whole-mount immunofluorescence (WM-IF) analysis of Kitl-tdTomato expression in the E8.5 (6-7sp) conceptus (maximum intensity 3D projection from a 500μm-thick Z-stack). Kitl-tdTomato expression (red) is seen in the YS, allantois (a; from which the umbilical artery derives), and para-aortic splanchnopleura (PAS) of Kitl-tdTomato::23GFP transgenic concepti (the embryo proper, from which the head has been removed, is outlined with a purple dashed line). The 23GFP transgene (green) labels the YS blood islands (bi; yellow dashed line); this GFP reporter is transcribed from a heterologous hsp68 promoter under the spatiotemporal control of the Runx1 hematopoietic +23 enhancer, resulting in GFP expression in all primitive erythroid(-fated) cells, hemogenic endothelium, and emerging HSPCs[2,74]. Endothelial cells (CD31+) are in white. Arrowheads indicate the paired aortae (pa). Purple boxed area is magnified in (B); yellow boxed area is magnified in (C). Scale bar: 100μm. N=2 embryos analyzed. Single 2.5μm-thick Z slice from the region highlighted in the purple box in (A). Arrowheads indicate Kitl-tdTomato+ CD31+ endothelial cells in one of the paired aortae (pa). Scale bar: 25μm. Single 2.5μm-thick Z slice from the region highlighted in the yellow box in (A), showing Kitl-tdTomato expression in the E8.5 YS BI. Arrowheads indicate Kitl-tdTomato+ CD31+ endothelial cells; the arrow indicates a Kitl-tdTomato+ perivascular cell. Scale bar: 25μm. Single 2.5μm-thick Z slice of 10.5 (35sp) YS (WM-IF) showing Kitl-tdTomato (red), Kit (white) and CD31 (green) expression. Arrowheads indicate Kitl-tdTomato+ CD31+ endothelial cells. N=3 embryos analyzed. Scale bar: 50μm."
NCBI gene IDs:
"GFP: 
Kitl: 
tdTomato: 
Runx1: 12394"
Figure legend: "Confocal immunofluorescence analysis of cryosections through the AGM region of E10.5 (34-36sp) Kitl-tdTomato transgenic embryos. Arrowheads indicate Kitl-tdTomato+ CD31+ endothelial cells (top), Kitl-tdTomato+ PDGFR-β+ (middle) or Kitl-tdTomato+ α-SMA+ (bottom) perivascular smooth muscle cells. Scale bars: 25μm or 50μm (PDGFR-β panel). N&gt;6 embryos analyzed."
NCBI gene IDs:
"tdTomato: 
Kitl: 17311"
Figure legend: "Confocal immunofluorescence analysis of Kitl-tdTomato expression in the E10.5 FL. A cryosection through a E10.5 (35-36sp) Kitl-tdTomato transgenic FL is shown. Arrowheads indicate Kitl-tdTomato+ CD31+ Kit- endothelial cells. Asterisks indicate Kitl-tdTomato+ CD31- Kit- mesenchymal cells or hepatoblasts. Note proximity of Kitl-tdTomato+ cells and Kit+ hematopoietic cells. Scale bars: 50μm (left panel), 25μm (magnified inset). N&gt;5 embryos analyzed."
NCBI gene IDs:
"Kitl: 
tdTomato: "