EMBO/SourceData huggingface dataset:
Figure legend: "QPCR validation of transcripts involved in the stem cell signalling pathway that are upregulated upon 5'-tsRNA knockdowns using ASOs. (n =2) Error bars represent SEM, significance was calculated using unpaired t-test."
NCBI gene IDs:
"tsRNA: "
Figure legend: "Quantification of the Igf2bp1 bound c-Myc mRNA between LIF- versus RA-treated mESCs (n = 2; see Fig. EV5F for duplicate data)."
NCBI gene IDs:
"c-Myc: 17869"
Figure legend: "Association of c-Myc transcript in different translating pools in RA treated mESCs as compared to LIF condition. (n = 2), Error bars represent SD, significance was calculated by one-tailed unpaired t-test."
NCBI gene IDs:
"c-Myc: 17869"
Figure legend: "Relative enrichment of c-Myc mRNA in translating (80S and Polysome) and non-translating (mRNPs) pools (fractionated from polysome profiling) between ASO-treated and Mock-treated RA-induced differentiating mESCs. (n =2) Error bars represent SD, significance was calculated using one-tailed unpaired t-test."
NCBI gene IDs:
"c-Myc: 17869"
Figure legend: "Relative levels of c-Myc mRNA in ASO-treated and Mock-treated RA-induced differentiating mESCs compared to LIF treated mESCs. (n = 3) Error bars represent SD, significance was calculated using one-tailed unpaired t-test."
NCBI gene IDs:
"c-Myc: 17869"
Figure legend: "Epistatic analysis of 5'-tsRNAs and IGF2BP1 in regulating Myc transcriptional reporter activity. (n = 3), Error bars represent SD, significance was calculated using unpaired t-test."
NCBI gene IDs:
"IGF2BP1: "
Figure legend: "C (i) Low magnification overview of the  spinal cord of a cilium  reporter line, Tg(actb2:arl13b-GFP) from a single confocal  slice. (ii) High resolution image sequence of boxed section in (i). One GFP-labelled cilium  moves from apical  surface  towards the basal  surface  of the spinal cord  (n = 13 cilia  from two experiments). Images are maximum projections from confocal  z-stacks. Data information: All scale bars = 10 µm."
NCBI gene IDs:
"GFP: 
actb2: 57935
arl13b: 286784"
Figure legend: "D (i) Low magnification overview of the spinal cord of a Tg(actb2:arl13b-GFP) embryo labelled  with centrin-RFP  from a single confocal  slice. (ii) Image sequence of boxed section in (i). A cilium and centrosome  move together from apical  surface  towards the  basal surface of the spinal cord (n = 2 cells from one experiment). Insets show high magnification of cilium-centrosome  pair. Images are maximum projections from confocal  z-stacks. Data information: All scale bars = 10 µm."
NCBI gene IDs:
"GFP: 
actb2: 57935
arl13b: 286784"
Figure legend: "F Distance between centrosome  and cilium  in Tg(actb2:arl13b-GFP) embryos  fixed and processed for immunohistochemistry   against GFP  to label the cilium  and γ-tubulin to label the centrosome. No difference was found in the distance between the two organelles when close to the  apical surface or away from the midline   (n = 50 cells per position from two experiments; P = 0.8279; midline  mean = 0.4956, s.d = 0.1125; away from midline  mean = 0.5004, s.d = 0.1077; Student's unpaired t-test). Bars show mean and standard deviation."
NCBI gene IDs:
"GFP: 
actb2: 57935
arl13b: 286784"
Figure legend: "A Transverse section from a confocal  z-stack showing the whole neural tube of a utr-mCherry embryo, showing localisation  to the  basal surface. B Transverse sections from a confocal  z-stack of a  non-apical progenitor labelled with a membrane  marker in a utr-mCherry embryo  to identify the basal surface of the spinal cord. Image sequence from confocal time-lapse shows the non-apical progenitor (-44m) undergoing mitosis to produce two neurons  (-22m), of which one is not in contact with the basal surface. Both neurons  extend nascent axons (0m). Data information: All scale bars = 5 µm."
NCBI gene IDs:
"mCherry: 
utr: "
Figure legend: "C Transverse sections from a confocal  z-stack of a neuron  at the time  of nascent axon initiation labelled with a membrane  marker in a utr-mCherry embryo  to identify the basal surface  of the spinal cord. Three different sections show the middle of the soma, the axon  initiation site, and the  axon tip. Green and magenta peaks in graphs show relative positions of cell membrane and basal surface, respectively. D Transverse sections from confocal  z-stacks of a neuron  at the time  of nascent axon initiation labelled with a membrane  marker in a utr-mCherry embryo  injected with  lamMO. Three different sections show the middle of the soma, the axon  initiation site, and the axon tip. Green and magenta peaks in graphs show relative positions of cell membrane and basal surface, respectively. E Graphs showing distance (µm) between the basal edge of the soma, the axon  initiation site or the axon tip and the  basal surface of the spinal cord. Measurements were performed by measuring between basal-most green and magenta peaks in graphs of relative grey values (C, D). Bars show mean and standard deviation. WT: n = 5 cells from one experiment; lamMO: n = 11 cells from two experiments. Soma: WT mean = 2.007 µm, s.d. = 1.055;  lamMO  mean = 1.871 µm, s.d. = 1.830. Student's two-tailed test, P = 0.880. Axon  initiation site: WT mean = 1.366 µm, s.d. = 3.13; lamMO mean = 2.353 µm, s.d. = 2.125. Student's two-tailed test, P = 0.468. Axon tip: WT mean = 0.637 µm, s.d. = 1.078; lamMO mean = 2.903 µm, s.d. = 2.0433. Student's two-tailed test, P = 0.037. Data information: All scale bars = 5 µm. * P &lt; 0.05."
NCBI gene IDs:
"mCherry: 
utr: 
lam: 286832"
Figure legend: "F Image sequence from confocal time lapse shows a neuron  in a Sly-/- embryo  labelled with membrane  and centrosome  markers before (-40m), during (0) and after axon  initiation (20m). Images are transverse reconstructions from confocal  z-stacks."
NCBI gene IDs:
"Sly: 286832"
Figure legend: "G Plots showing axon  position on the soma  relative to the cell centroid  at 0,0 for dorsal and transverse view in Sly-/- embryos  (n = 18 cells from three experiments). Axon  position is not random (dorsal view P &lt; 0.001, mean = 98.8o; transverse view P &lt; 0.001, mean = 161.1o; Moore's modification of Rayleigh's test). H Plots showing merge of WT and Sly-/- axon  positions on the cell body relative to  cell centroid  at 0,0 for dorsal and transverse views. Axon  positions in WT and Sly-/- are not significantly different in dorsal view (0.2 &lt; P &lt; 0.5) but are in transverse view (P &lt; 0.001; Batschelet's alternative to Hotelling test). WT: n = 86 cells from eight experiments; Sly-/-: n = 18 cells from three experiments."
NCBI gene IDs:
"Sly: 286832"
Figure legend: "I Graph showing distance between centrosome  and base of axon  at time of axon  initiation in WT and Sly-/- embryos. Bars show mean and standard deviation. WT: n = 26 cells from three experiments, mean = 10.13 µm, s.d. = 3.35. Sly-/-: n = 15 cells from two experiments, mean = 12.41 µm, s.d. = 3.281. One-way ANOVA, P = 0.123."
NCBI gene IDs:
"Sly: 286832"
Figure legend: "J Plots showing centrosome  position relative to the  cell centroid at 0,0 for dorsal and transverse view in Sly-/- embryos  (n = 15 cells from two experiments). Centrosome  position is not random (dorsal view P &lt; 0.001, mean = -129.0o; transverse view 0.001 &lt; P &lt; 0.005, mean = -57.0o; Moore's modification of Rayleigh's test). K Plots showing merge of WT and Sly-/- centrosome  positions on the cell body  relative to cell centroid  at 0,0 for dorsal and transverse views. Centrosome  positions are not significantly different between WT and Sly-/- (dorsal view 0.2 &lt; P &lt; 0.2, transverse view 0.1 &lt; P &lt; 0.2; Batschelet's alternative to Hotelling test). WT: n = 26 cells from three experiments; Sly-/-: n = 15 cells from two experiments."
NCBI gene IDs:
"Sly: 286832"
Figure legend: "L Plots showing the positions of the centrosome  and base of the axon  in Sly-/- embryos  at the time of axon  initiation relative to the cell centroid at 0,0 for dorsal and transverse view (n = 15 cells from two experiments). Left-hand plots: centrosome  position is not random (dorsal view P &lt; 0.001, mean = -129.0o; transverse view P &lt; 0.001, mean = -57.0) and axon  position is not random (dorsal view P &lt; 0.001, mean = 95.1o; transverse view P &lt; 0.001, mean = 168.8o, Moore's modification of Rayleigh's test). Centrosome  and axon  positions are significantly different (dorsal view 0.001 &gt; P; transverse view 0.001 &gt; P; Moore's test for paired data). Right-hand plots: vectors connecting centrosome  and  nascent axon from the same cell are not random (dorsal view P &lt; 0.001, mean = 66.1o; transverse view P &lt; 0.001, mean = 150.9o)."
NCBI gene IDs:
"Sly: 286832"
Figure legend: " D) RT-qPCR analysis of expression of CaSR in human spinal tissue. n=3, Student's t-test. "
NCBI gene IDs:
"CaSR: 846"
Figure legend: "(D) RT-qPCR analysis of expression of CaSR in spinal ligament tissuse. n=3, Student's t-test."
NCBI gene IDs:
"CaSR: 12374"
Figure legend: " (D) RT-qPCR analysis of expression of CaSR in hind paw tissue. n=3, Student's t-test. "
NCBI gene IDs:
"CaSR: 12374"
Figure legend: " (A) RT-qPCR and Western Blot analyses of CaSR expressions in MC3T3-E1 cells treated with IL-1β. n=3, one‐way ANOVA, Bonferroni post hoc. "
NCBI gene IDs:
"CaSR: 12374
IL-1β: 16176"
Figure legend: " (B) RT-qPCR and Western Blot analyss of CaSR expressions in MC3T3-E1 cells treated with TNFα. n=3, one‐way ANOVA, Bonferroni post hoc. "
NCBI gene IDs:
"CaSR: 12374
TNFα: 21926"
Figure legend: " (C) RT-qPCR and Western Blot analyses of CaSR expressions in MC3T3-E1 cells treated with IL-17A. n=3, one‐way ANOVA, Bonferroni post hoc. "
NCBI gene IDs:
"CaSR: 12374
IL-17A: 16171"
Figure legend: " (D) RT-qPCR and Western Blot analyses of CaSR expressions in MC3T3-E1 cells treated with IL-22. n=3, one‐way ANOVA, Bonferroni post hoc. "
NCBI gene IDs:
"CaSR: 12374
IL-22: 50929"
Figure legend: " (E) RT-qPCR and Western Blot analyses of CaSR expressions in MC3T3-E1 cells treated with IL-23. n=3, one‐way ANOVA, Bonferroni post hoc. "
NCBI gene IDs:
"CaSR: 12374
IL-23: 83430"
Figure legend: "(A-D) Images of a representative wild-type adult eye (A) or adult eyes containing mtm mutant clones (B and C). Quantification of relative eye sizes (n=10) (D)."
NCBI gene IDs:
"mtm: 33845"
Figure legend: "(F-G) A pupal eye disc containing mtm mutant clones (marked by the absence of GFP) was stained for Dlg. Note the increased interommatidial cells in the mtm mutant clone. Quantification of numbers of interommatidial cells per ommatidium (n=10) (G)."
NCBI gene IDs:
"mtm: 33845"
Figure legend: "(K) RNAi of Mtm reduces Yki phosphorylation. Kc167 cells were incubated with dsRNA of gfp or mtm for three days before western blot analysis. Total cell lysates were probed with anti-phospho-Yki (p-Yki) antibody. Note that RNAi of mtm decreased Yki phosphorylation."
NCBI gene IDs:
"gfp: 
mtm: 33845
Mtm: 33845"
Figure legend: "PI(3)P expression levels detected by a 2xFYVE-GFP reporter in a third instar larval wing disc (A) and eye disc (B) containing mtm mutant clones (marked by the absence of RFP). Ten different mtm clones and surrounding regions in three biological replicates were used for the analysis. Note the strong increases of PI(3)P levels in the mtm mutant clones."
NCBI gene IDs:
"mtm: 33845"
Figure legend: "Quantification of the mean FYVE-GFP fluorescence intensity of mtm mutant clones and surrounding wild-type (WT) cells are shown (C). Ten different mtm clones and surrounding regions in three biological replicates were used for the analysis. Note the strong increases of PI(3)P levels in the mtm mutant clones."
NCBI gene IDs:
"mtm: 33845"
Figure legend: "(B, C) Percentage of GAPDH or 5.8 rRNA levels quantified by  qPCR in reactions containing increasing doses of (PR)20 (n=3).Data represent mean values ± SEM. Data information: **, p&lt;0.01; ***, p&lt;0.001; ****, p&lt;0.0001; t-test."
NCBI gene IDs:
"GAPDH: 2597
5.8 rRNA: 110255166///110255170///106632261///106632262///106632263"
Figure legend: "(A) Cells were grown to mid‐exponential phase and then incubated in the presence of 3.5% galactose to induce expression of GFP-Atg8. Aminopeptidase I (API) maturation was tested in cells incubated in control or starvation medium by western blot analysis using anti‐Atg8 (right bottom panel), anti‐GFP (left bottom panel) or anti‐API antibodies (upper panel)."
NCBI gene IDs:
"Atg8: 852200"
Figure legend: "(A) Atg8WT-HA, Atg8F77A-HA, Atg8F79A-HA or Atg8F77/79A-HA was transformed into Δatg8 cells. Cell extracts were prepared and subjected to 13.5% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) containing 6 M urea, followed by immunoblot analysis with anti-Atg8 antibodies."
NCBI gene IDs:
"Atg8: 852200
atg8: 852200"
Figure legend: "(C) Pull‐down assay of Atg8 mutants with Atg4. His-Atg4 was attached to cobalt beads (Talon metal‐affinity resin), washed and incubated with total cell extracts of Atg8 mutants. Elution was subjected to SDS-PAGE followed by western blot analysis using anti‐Atg8 and anti‐Atg4 antibodies."
NCBI gene IDs:
"Atg8: 852200"
Figure legend: "(D) Localization of green fluorescent protein (GFP)-Atg8wt and GFP-Atg877/79 cells carrying Atg4 plasmid under normal (control) and starvation conditions. Arrows point to the pre‐autophagosomal structure, and arrowheads point to GFP within the vacuoles."
NCBI gene IDs:
"Atg4: 855498
Atg8: 852200"
Figure legend: "(A) Δatg8 cells transformed with GFP-Atg8WT, GFP-Atg8L50A or GFP-Atg8Y49A constructs were grown to mid‐exponential phase and then incubated in the presence of 3.5% galactose to induce expression of GFP-Atg8. API maturation was tested using anti‐Atg8 (right bottom panel), anti‐GFP (left bottom panel) or anti‐API antibodies."
NCBI gene IDs:
"Atg8: 852200"
Figure legend: "(C) Atg8WT-HA, Atg8L50A-HA or Atg8Y49A-HA was transformed into Δatg8 and Δatg8Δatg4 double knockout strains and cell extracts were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblot analysis using anti‐HA antibodies (lower panel; asterisk represents nonspecific band)."
NCBI gene IDs:
"Atg8: 852200"
Figure legend: "(D) Atg8WT, Atg8L50A or Atg8Y49A was transformed into Δatg8 and Δatg8Δatg4 double knockout strains and grown under control or starvation conditions for 4 h. Cell extracts were subjected to 13.5% SDS-PAGE containing 6 M urea, followed by immunoblot analysis using anti‐Atg8‐N15 antibodies. GFP, green fluorescent protein; WT, wild type."
NCBI gene IDs:
"Atg8: 852200"
Figure legend: "A Scatterplot representing differences in ssGSEA immune signatures in TERThigh versus TERTlow tumours (n = 4,632 tumours in each group) across TCGA. -log10(padj) for enrichment of ssGSEA immune signatures in TERThigh versus TERTlow tumours is shown on the y-axis. Signatures more highly represented in TERThigh tumours are shown on the right, while those in TERTlow tumours are shown on the left."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "D-F RT-qPCR analysis of interferon-related genes (D), western blot analysis of TERT, TBK1, pTBK1, IRF3, pIRF3, and GAPDH levels (E), and ELISA of IFNβ and CXCL10 in culture supernatant (F) in U2OS and HeLa cells ectopically expressing TERT-WT or TERT-K626A."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "D RT-qPCR analysis of TA-ERVs in U2OS and HeLa cells ectopically expressing TERT-WT or TERT-K626A. E RT-qPCR analysis of TA-ERVs in HeLa and HCT116 cells with TERT knockdown. F"
NCBI gene IDs:
"TERT: 7015"
Figure legend: "F-H RT-qPCR analysis of interferon-related genes and TA-ERVs (F), western blot analysis of TERT, TBK1, pTBK1, IRF3, pIRF3, and GAPDH levels (G), and ELISA of IFNβ and CXCL10 in culture supernatant (H) in U2OS cells ectopically expressing TERT-WT or TERT-K626A using Dox-inducible system. GFP was used as negative control. Relative levels of pTBK1 and pIRF3 were quantified with ImageJ software and normalized to GAPDH, as indicated at the bottom of the blot (G)."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "D MeDIP-qPCR analysis of TA-ERVs sites in HeLa cells ectopically expressing TERT-WT or TERT-K626A. Primer sets recognizing exon 8 of the human ZC3H13 gene and a gene desert on chromosome 12 were used for positive and negative controls, respectively."
NCBI gene IDs:
"TERT: 7015
ZC3H13: 23091"
Figure legend: "E ChIP-qPCR for TERT and Sp1 targets on TA-ERVs in U2OS cells with TERT ectopic expression and in HeLa cells. GAPDH promoter was used as a negative control, and VEGF promoter was used as a positive control."
NCBI gene IDs:
"GAPDH: 
TERT: 7015
VEGF: 7423"
Figure legend: "A Expression of murine ERVs in blood of WT and G1 Tert-/- mice. n = 5 mice per group. B Expression of MMERGLN_LTR and MMERGLN-int in livers of WT and G1 Tert-/- mice treated with ENU or saline (control). n = 3 mice per group. C"
NCBI gene IDs:
"Tert: 21752"
Figure legend: "D Heat map showing log2 fold change differences of interferon-related genes expression between ENU treatment and control (saline) in WT and G1 Tert-/- mice by RNA-seq. n = 3 mice per group. E Heat map showing log2 fold change differences of cytokine/chemokine between ENU treatment and control (saline) in WT and G1 Tert-/- mice by cytokine/chemokine array. n = 5 mice per group. F"
NCBI gene IDs:
"Tert: 21752"
Figure legend: "B TCGA fragments per kilobase million (FPKM) values of TERT, CXCL10, ISG15, and OASL in tumours grouped in TERThigh (n = 500) and TERTlow groups (n = 500). Data represent mean ± SD. P-values were analysed using unpaired two-tailed Student's t-tests."
NCBI gene IDs:
"CXCL10: 3627
ISG15: 9636
OASL: 8638
TERT: 7015"
Figure legend: "C TCGA FPKM values of interferon-related genes in tumours grouped in TERThigh (n = 500) and TERTlow (n = 500) groups. Heat map showing mean FPKM values of each gene."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "Expression of TERT (D) in TERThigh and TERTlow cancer types (6 types in each). ACC (adrenocortical carcinoma), n = 79. KICH (kidney chromophobe), n = 66. KIRP (kidney renal papillary cell carcinoma), n = 291. LGG (brain lower grade glioma), n = 532. PRAD, n = 502. THCA, n = 513. BLCA (bladder urothelial carcinoma), n = 414. CESC (cervical squamous cell carcinoma and endocervical adenocarcinoma), n = 306. DLBC (lymphoid neoplasm diffuse large B-cell lymphoma), n = 48. LUSC (lung squamous cell carcinoma), n = 502. OV (ovarian serous cystadenocarcinoma), n = 430. All boxplots include the median line, box indicates the interquartile range (IQR) and whiskers denote the 1.5 × IQR. P-values were analysed using two-tailed Mann-Whitney U test."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "Expression of DDX58, IFIH1, CXCL10, IFI44, ISG15, and OASL (E) in TERThigh and TERTlow cancer types (6 types in each). ACC (adrenocortical carcinoma), n = 79. KICH (kidney chromophobe), n = 66. KIRP (kidney renal papillary cell carcinoma), n = 291. LGG (brain lower grade glioma), n = 532. PRAD, n = 502. THCA, n = 513. BLCA (bladder urothelial carcinoma), n = 414. CESC (cervical squamous cell carcinoma and endocervical adenocarcinoma), n = 306. DLBC (lymphoid neoplasm diffuse large B-cell lymphoma), n = 48. LUSC (lung squamous cell carcinoma), n = 502. OV (ovarian serous cystadenocarcinoma), n = 430. All boxplots include the median line, box indicates the interquartile range (IQR) and whiskers denote the 1.5 × IQR. P-values were analysed using two-tailed Mann-Whitney U test."
NCBI gene IDs:
"CXCL10: 3627
DDX58: 23586
IFI44: 10561
IFIH1: 64135
ISG15: 9636
OASL: 8638
TERT: 7015"
Figure legend: "G Representative sections of immunohistochemical staining of CD4 and FOXP3 in TERT-ERVshigh and TERT-ERVslow colon tumours. Red arrowheads indicate FOXP3+ cells. Scale bar = 100 μm."
NCBI gene IDs:
"TERT: 7015"
Figure legend: "(A) Design of a functional dynamin mutant to probe PHD stalk interactions. Minimal structure of the PHD and the PHD-Stalk interface showing the location of important residues discussed in this study. The unresolved loop L1NS in the stalk hosts the IAEDANS label and is sensitive to the two tryptophans (W525 and W542) in the PHD. The distance between two introduced cysteines (S357C and native C602) used to construct a double cysteine Dyn1 is also shown."
NCBI gene IDs:
"dynamin: "
Figure legend: "(B) Histogram shows basal and assembly-stimulated GTPase activities of non-crosslinked Dyn1CC vs crosslinked Dyn1Closed and Dyn1WT. Inset shows crosslinking of the PHD and stalk domains in Dyn1Y354C/S607C (Dyn1CC) with the bifunctional crosslinker, MTS-2-MTS, to yield Dyn1Closed, which migrates more slowly on SDS-PAGE, as well as liposome binding of Dyn1CC compared to Dyn1Closed (Bound, B; Unbound, UB)."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(C) Ability of Dyn1WT, Dyn1CC and Dyn1Closed to catalyze membrane fission and vesicle release from SUPER templates."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(D) Size exclusion chromatographic profiles of Dyn1CC, Dyn1Closed at room temperature or after incubation for 10 min at 37°C."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(F) Negative-stain electron micrographs of Dyn1WT, Dyn1ΔΔ and Dyn1Closed assembled onto lipid nanotubes. Scale bar = 200 nm"
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(G) Curvature dependent liposome-stimulated GTPase activity of Dyn1WT and Dyn1ΔΔ."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(H) Ability of Dyn1WT and Dyn1ΔΔ to catalyze membrane fission and vesicle release from SUPER templates."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(A) Temperature dependence of basal and lipid stimulated GTPase activity of Dyn1WT and Dyn2S619L."
NCBI gene IDs:
"Dyn1: 1759
Dyn2: 1785"
Figure legend: "(B) Temperature sensitive membrane fission activity of Dyn1WT, Dyn2WT and Dyn2S619L measured using SUPER templates."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(C) Detection of Dyn2 PHD-stalk interactions by FRET using IAEDANS-labeled Dyn2L354C."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(D) Curvature dependent membrane binding and consequent opening of PHD in Dyn1Y354C-IAEDANS and Dyn2L354C-IAEDANS."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(E) Differential temperature dependence of PHD-stalk FRET for WT and S619L mutant Dyn2IAEDANS. Data are presented as relative FRET distances assuming a single donor/acceptor pair."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(F) Differential solvent exchange kinetics of Dyn1S619L at ambient temperature compared to Dyn1WT under identical conditions. Yellow coloring indicates a significant increase in HDX. Quantitative data on the individual peptides used to generate this map are provided in (Appendix Fig. S5)."
NCBI gene IDs:
"Dyn1: 1759"
Figure legend: "(A,B) Transferrin receptor (TfnR) uptake, a measure of CME, shown as % uptake of total steady-state surface TfnR for control H1299 cells, and H1299 cells stabily expressing low amounts of siRNA-resistant Dyn2WT-EGFP (A) or Dyn2S619L-EGFP (B) with or without treatment of siRNA-directed towards endogenous Dyn2."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(C) Fixed cell TIRF-M images of Dyn2WT-EGFP or Dyn2S619L-EGFP expressing H1299 cells with or without Dyn2-siRNA treatment showing co-localization with clathrin light chain immunostained rabbit anti-CLC and Alexa 547-conjugated secondary antibodies."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(D) Percentage of Dyn2-EGFP positive clathrin coated pits detected by live cell TIRF-M and automated master/slave image analysis (Aguet et al, 2013)."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(E) Lifetime analysis of CCPs which are positive for EGFP-Dyn2WT or EGFP-Dyn2S619L with or without siRNA-mediated specific knockdown of endogenous Dyn2."
NCBI gene IDs:
"Dyn2: 1785"