EMBO/SourceData huggingface dataset:
Figure legend: "(A) Bulk protein degradation was tested in HeLa cells that were transfected with non‐targeting siRNA (control siRNA), a pool of GABARAP/GATE‐16 siRNAs, or a pool of LC3 siRNAs. The rate of long‐lived protein degradation was measured in cells incubated in either αMEM (control) or EBSS (starvation) medium, in the presence or absence of 10 mM of 3‐MA, 72 h after siRNA transfection. Values expressing the protein degradation percentage are represented as the mean±s.d. of three determinations."
NCBI gene IDs:
"GABARAP: 11337
GATE‐16: 11345"
Figure legend: "(B) HeLa cells stably expressing GFP‐LC3B (left panel) or GFP‐GATE‐16 (right panel) were transfected with a pool of GABARAP/GATE‐16 siRNAs or a pool of LC3, respectively, and with non‐targeting siRNA. Seventy‐two hours after transfection, the cells were collected after 5 h of starvation and the relative level of GFP‐LC3B or GFP‐GATE‐16 was measured using flow cytometry. Values represent the mean±s.d. of three experiments."
NCBI gene IDs:
"GABARAP: 11337
GATE‐16: 11345
LC3B: 81631"
Figure legend: "(A) HeLa cells stably expressing YFP‐Atg5 were transfected with non‐targeting siRNA (control siRNA), a pool of LC3 siRNAs, or a pool of GABARAP/GATE‐16 siRNAs using DharmaFect reagent. Seventy‐two hours after transfection, the cells were incubated for 2 h in EBSS medium, fixed, and immunostained with anti‐Atg16 antibodies. Quantification of Atg5 and Atg16‐labelled puncta structures from three independent experiments is presented on the right panel. Scale bar: 20 μm."
NCBI gene IDs:
"Atg5: 9474
GABARAP: 11337
GATE‐16: 11345"
Figure legend: "(B) HeLa cells stably expressing YFP‐Atg5 were treated as in (A) and monitored by live microscopy. Quantification of the lifespan of Atg5‐labelled puncta structures is presented. Mean±s.d. of three independent experiments is presented. *P0.05, **P0.001."
NCBI gene IDs:
"Atg5: 9474"
Figure legend: "(C) YFP‐Atg5 cells were transfected with siRNA pool and starved as in (A). Cryo sections of fixed cells were immunolabelled using anti‐GFP antibodies and analysed by TEM as described in 'Materials and methods'. Quantification of the Atg5‐labelled structures size is presented at the right panel. *P0.05, **P0.001."
NCBI gene IDs:
"Atg5: 9474"
Figure legend: "(A) Control HeLa cells and HeLa cells stably expressing GFP‐LC3B or GFP‐GATE‐16 were starved for 2 h in EBSS medium fixed and immunostained with anti‐Atg16 antibodies. Quantification of Atg16‐labelled puncta structures from three independent experiments is presented at the right panel. Arrowheads represent Atg‐16‐labelled puncta. Scale bar: 20 μm."
NCBI gene IDs:
"GATE‐16: 11345
LC3B: 81631"
Figure legend: "(B) HeLa cells stably expressing GFP‐LC3B (left panel) or GFP‐GATE‐16 (middle panel) were starved for 2 h in EBSS medium. The cells were fixed and their cryo sections were immunolabelled as in ( Figure 4B). Quantification of the size of GFP‐labelled structures (right panel) from three independent experiments is presented. *P0.05, **P0.001."
NCBI gene IDs:
"GATE‐16: 11345
LC3B: 81631"
Figure legend: "(A, B) Control HeLa cells and HeLa cells stably expressing GFP‐LC3B (A) or GFP‐GATE‐16 (B) were transfected with the reciprocal siRNA pools using DharmaFect reagent. After 72 h interval, the cells were incubated for 2 h in EBSS, subjected to immunostaining with anti‐Atg16 after fixation, and analysed by confocal microscopy. Quantification of cells containing Atg16 structures larger than 2 μm (A) or Atg16‐labelled puncta structures (B) from three independent experiments in comparison to control and to G to A mutants (D) is presented at the lower panel. Scale bar: 20 μm."
NCBI gene IDs:
"GATE‐16: 11345
LC3B: 81631"
Figure legend: "(C) HeLa cells stably expressing silent GFP‐LC3B or GFP‐GATE‐16 were transfected with their siRNA pools using DharmaFect reagent. After 72‐h interval, the cells were incubated for 2 h in EBSS medium and subjected to immunostaining with anti‐Atg16 and anti‐p62 antibodies followed by fixation. The cells were analysed by confocal microscopy. Arrows represent cells, which do not express GFP proteins whereas arrowheads represent cells, which express GFP‐proteins. Quantification of Atg16‐labelled puncta structures from three independent experiments is presented at the right panel. Scale bar: 20 μm."
NCBI gene IDs:
"GATE‐16: 11345
LC3B: 81631"
Figure legend: "(D) HeLa cells stably expressing GFP‐LC3BG120A or GFP‐GATE‐16G116Awere transfected with either pool of GABARAP/GATE‐16 siRNAs or LC3 siRNAs, respectively, and a non‐targeting siRNA. The cells were starved for 2 h, fixed, and immunostained with anti‐Atg16 and anti‐p62 antibodies. Quantification is presented in (A, B) at the lower panels. Scale bar: 20 μm."
NCBI gene IDs:
"GABARAP: 11337
GATE‐16: 11345
LC3B: 81631"
Figure legend: "(E) HeLa cells stably expressing GFP‐LC3B (left panel) or GFP‐GATE‐16 (right panel) were transfected with the reciprocal siRNA pool or with non‐targeting siRNA and starved for 2 h in EBSS medium. The cells were fixed and their cryo sections were immuno‐labelled using anti‐GFP antibodies and analysed by TEM as described in 'Materials and methods'. Quantification of cells containing Atg16 structures larger than 2 μm (left panel) or the size of Atg16‐labelled structures (right panel) is presented."
NCBI gene IDs:
"GATE‐16: 11345
LC3B: 81631"
Figure legend: "(A) Control HeLa cells and HeLa cells stably expressing GFP‐LC3B or GFP‐GATE‐16 were transfected with HsAtg4AC77A‐Myc‐His6. After 48 h interval, the cells were incubated for 2 h in EBSS, and subjected to immunostaining with anti‐Atg16 and anti‐myc followed by confocal microscopy analysis. Quantification of cells containing Atg16 structures larger than 2 μm from three independent experiments is presented at the right panel. Scale bar: 20 μm."
NCBI gene IDs:
"Atg4A: 115201
GATE‐16: 11345
LC3B: 81631"
Figure legend: "(B) HeLa cells were transfected with HsAtg4AC77A‐Myc‐His6 or empty vector (mock). After a 48 h interval, the cells were incubated for 2 h in EBSS, fixed, and their ultrathin sections were analysed by TEM as described in 'Materials and methods'. *P0.05."
NCBI gene IDs:
"Atg4A: 115201"
Figure legend: "(D) mRNA expression of Il-1β; n= 5(RD), 7(HFD), 7(HFD+AB), 3(RD+AB); CI 95%, Il-6; n= 5(RD), 5(HFD), 4(HFD+AB), 4(RD+AB); CI 95%, Tnf-α; n= 6(RD), 9(HFD), 4(HFD+AB), 5(RD+AB); CI 95% and Vegf-a; n= 6(RD), 5(HFD), 5(HFD+AB), 6(RD+AB); CI 95% in choroids."
NCBI gene IDs:
"Il-1β: 16176
Il-6: 16193
Tnf-α: 21926
Vegf-a: 22339"
Figure legend: "(J) mRNA expression of Il-1β; n= 5(RDxRDT), 4(HFDxHFDT), 5(HFDxRDT), Il-6; n= 5(RDxRDT), 4(HFDxHFDT), 4(HFDxRDT); CI 99%, Tnf-α; n= 5(RDxRDT), 4(HFDxHFDT), 4(HFDxRDT); CI 95% and Vegf-a; n= 5(RDxRDT), 4(HFDxHFDT), 5(HFDxRDT); CI 95% in choroids."
NCBI gene IDs:
"Il-1β: 16176
Il-6: 16193
Tnf-α: 21926
Vegf-a: 22339"
Figure legend: "Volcano plot representation of AFG3L2E408Q-FLAG interaction  partners. Mitochondria  isolated from  Flp-In HEK293T-REx cells expressing  AFG3L2E408Q-FLAG  were subjected to immunoprecipitation. Co-purifying proteins were identified by quantitative  mass spectrometry (MS) (n=5 independent experiments) followed by an unpaired two-sided t-test and permutation-based FDR estimation to correct for multiple testing (FDR&lt;0.05. Significantly enriched MitoCarta 3.0 proteins are colored in red, gene names indicate previously verified AFG3L2 interactors  and TMBIM5  (GHITM, MICS1)."
NCBI gene IDs:
"FLAG: 
AFG3L2: 10939"
Figure legend: "Representative immunoblot  of HeLa  wildtype (WT) cells transfected with scrambled siRNA (control) or siRNA targeting TMBIM5  and treated with the indicated drugs for 16 h. Control (0.1% DMSO), actinomycin D  (1 µM), venetoclax  (1 µM), A-1155463 (1 µM), staurosporine  (1 µM), thapsigargin  (2 µM). n=3 independent experiments."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "Representative immunoblot  of HeLa  WT and TMBIM5-/- cells transfected with scrambled siRNA (control) or siRNA targeting MCU  for 72 h. Where indicated samples were treated with staurosporine  (STS; 1 µM) for 16 h. (n=3 independent experiments)."
NCBI gene IDs:
"TMBIM5: 27069
MCU: 90550"
Figure legend: "Viability  of WT and TMBIM5-/- cells (depleted of MCU  when indicated) after incubation with staurosporine  (STS; 0.1 µM) for 16 h. Cell viability was assessed cytofluorimetrically  monitoring NucView488 and RedDot2 staining  and expressed as percentage of all cells. n=3 independent experiments; two-tailed t-test; mean ±SD. A p-value of &lt;0.05 was considered statistically significant."
NCBI gene IDs:
"TMBIM5: 27069
MCU: 90550"
Figure legend: "WT and TMBIM5-/- HeLa  cells were transfected with the indicated scrambled siRNA (control) and siRNA targeting MCU  for 48 h and incubated with staurosporine  (0.1 µM) for 16 h in the presence of Z-VAD-FMK  (50 µM) and epoxomicin  (1 µM) to prevent apoptosis. Cells were subjected to immunofluorescence  analysis with antibodies directed against cytochrome c (green) and TOMM20  (magenta). *Cells with cytosolic cytochrome c. (n=3 independent experiments; number of cells &gt; 150 each experiment)."
NCBI gene IDs:
"TMBIM5: 27069
MCU: 90550"
Figure legend: "Fluorescence (F) F/F0 ratio quantifications, where F0 is the average of the first ten seconds and F is the fluorescence peak measured after thapsigargin  (2 µM) administration (left panel). The right shows mtRED-GECO  ratio traces of HeLa  cells transfected with scrambled siRNA (control) and siRNA targeting TMBIM5. Each measurement was performed in at least 10 cells from 6 different preparations. Mean ±SD; two-tailed t-test. A p-value of &lt;0.05 was considered statistically significant."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "Matrix pH  was measured with  Sypher3smito  in wildtye (WT) HeLa  cells transfected with scrambled siRNA (control) or siRNA targeting TMBIM5  for 72 h. The probe was calibrated in KRB buffer by the stepwise addition/removal of permeant weak acid. Each measurement was performed in at least 80 cells from 7 different preparations. Mean ±SD; two-tailed t-test. A p-value of &lt;0.05 was considered statistically significant."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "Mitochondrial membrane potential was monitored with TMRM   in WT and TMBIM5-/- HeLa  cells. Fluorescent intensity was calculated as ratio to the value upon CCCP  addition (15 µM). Values are expressed as mean ±SD relative to control. Each measurement was performed in triplicate from 4 different preparations."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "Matrix pH  was measured with  Sypher3smito  in WT HeLa  cells transiently expressing TMBIM5  or TMBIM5D294K D325K for 24 h. The ratio of absorbance at 488 nm and 405 nm is shown in arbitrary units (a.u.). Each measurement was performed in at least 20 cells from 3 different preparations. Representative immunoblot  of WT HeLa  cells transfected with TMBIM5  or TMBIM5D294K D325K for 24 h (right panel). Mean ±SD; two-tailed t-test. A p-value of &lt;0.05 was considered statistically significant."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "Alterations in mitochondrial  proteome in WT and TMBIM5-/- HeLa  cells upon depletion of AFG3L2. WT and TMBIM5-/- HeLa  cells were transfected with scrambled siRNA (control) or siRNA targeting AFG3L2  for 48 h. Proteins, which were downregulated in the absence of TMBIM5  and whose steady state levels was altered upon depletion of AFG3L2, were identified using a two-way ANOVA. An interaction p-value of 0.01 was used as a cutoff. Log2-transformed LFQ intensities of MitoCarta 3.0 proteins were Z-Score normalized and visualized in a hierarchical cluster analysis (Euclidean distance, complete method). The dendrogram is omitted. Proteins accumulating upon depletion of AFG3L2  (cluster 1, C1) and proteins whose steady state levels are decreased upon depletion of AFG3L2  (cluster 2, C2) are shown."
NCBI gene IDs:
"AFG3L2: 10939
TMBIM5: 27069"
Figure legend: "Representative immunoblot  of TMBIM5-/- HeLa  cells transfected with scrambled siRNA (control) or siRNA targeting AFG3L2  for 48 h. Samples were treated with the protein synthesis inhibitor cycloheximide  (CHX; 10 μg/ml) and collected at the indicated  time points (n=3 independent experiments)."
NCBI gene IDs:
"AFG3L2: 10939
TMBIM5: 27069"
Figure legend: "Representative immunoblot  of WT HeLa  cells transfected with scrambled siRNA (control) and siRNA targeting AFG3L2  for 48 h, treated when indicated with oligomycin  (10 μM) for 16 h. Quantification of TMBIM5  levels is shown on the right panel. TMBIM5  levels in untreated WT cells was set to 1 (n=3 independent experiments; mean ±SD)."
NCBI gene IDs:
"AFG3L2: 10939"
Figure legend: "TMBIM5  abundance determined by mass spectrometry  in WT HeLa  cells treated with oligomycin  (10 µM; 16 h) and depleted of AFG3L2  when indicated. n=5 independent experiments. P-value was calculated using a two-sided unpaired t-test. The central band of each box is the median value, and the box defines the 25th (lower) and 75th (higher) quantile. The whiskers represent the minimum and maximum value in the data excluding outliers (greater than 1.5 * inter quantile range distance to median - no outliers found here)."
NCBI gene IDs:
"AFG3L2: 10939"
Figure legend: "Zoom in to selected clusters of hierarchical clustering of Z-Score normalized log2 LFQ intensities. Proteins that were significantly (FDR&lt;0.05) accumulated between HeLa  WT and TMBIM5-/- cells after oligomycin  treatment (10 µM) for 16 h were clustered using Euclidean distance and the complete method on protein features. The figure shows identified cluster C4 and C5 with gene names and MitoCarta 3.0 localization  annotations. The row dendrogram is omitted."
NCBI gene IDs:
"TMBIM5: 27069"
Figure legend: "B, Same as in A, with the exception that cells expressing flag-praja2rm or praja2 deletion mutants (praja21-530, praja21-630 and praja2Δ530-630) were included in the analysis."
NCBI gene IDs:
"flag: 
praja2: 9867"
Figure legend: "E. Same as in C, with the exception that cells were co-transfected with flag-OFD1 vector and with control siRNA (siCNT) or siRNA targeting praja2. F. Quantitative analysis of the experiments shown in E. A mean value ± SD of three independent experiments is shown. Student's t test, *p&lt;0,05. G "
NCBI gene IDs:
"flag: 
OFD1: 8481
praja2: 9867"
Figure legend: "C. HEK293 cells  transfected  with siRNAs were serum deprived, pre-treated with cycloheximide and stimulated with FSK at different time point. Lysates were immunoblotted for endogenous praja2 and OFD1. Hsp90 was used as loading control. D. Quantitative analysis of the experiments shown in C. A mean value ± SD of three independent experiments is shown. Student's t test * p&lt;0,05; **&lt;0,01 versus sipraja2 (+FSK) and basal values (0). E "
NCBI gene IDs:
"praja2: 9867"
Figure legend: "H. Immunoblot analysis of flag-OFD1 or flag-E97G mutant in HEK293 cells stimulated with  FSK as in C. I. Quantitative analysis of the experiments shown in H. A mean value ± SD of three independent experiments is shown. Student's t test, *p&lt;0,05 versus E97G mutant (+FSK) and basal values (0). "
NCBI gene IDs:
"flag: 
OFD1: 8481"
Figure legend: "C. HEK293 cells expressing flag-OFD1 or flag-S735A were serum deprived and subjected to staining analysis for acetylated α-tubulin, flag and DRAQ5. Images were collected by super-resolution microscopy (upper panels). Where indicated, flag-OFD1 or flag-S735A was co-transfected  with cilia-APEX-GFP vector (middle panels). NIH3T3 expressing flag-OFD1 or flag-S735A were  stained with anti-ARL13B and anti-flag antibodies. D, E. Statistical analysis of the experiments shown in D. A mean value ± SD of three independent experiments is shown. For each experimental group, a minimum of 40 cilia were analyzed. Student's t test p***&lt;0,001. F "
NCBI gene IDs:
"flag: 
OFD1: 8481"
Figure legend: "F. HEK293 cells  transiently transfected  with flag-OFD1 or flag-S735A were serum  deprived, treated with FSK (6 hours) and stained for flag, acetylated-tubulin and DRAQ5. G. Statistical analysis of the experiments shown in F. A mean value ± SD of three independent experiments is shown. Cells analysed for each experiment: 300 for NT, 80 for  flag-OFD1 and 80 for flag-S735A. Student's t test, p*&lt;0,05, p**&lt;0,01. H "
NCBI gene IDs:
"flag: 
OFD1: 8481"
Figure legend: "B. Confocal images of cilia  of the  neural tube cells in the wild-type, Ol-TBC1D31 KD, Ol-TBC1D31 KD + hTBC1D31, wild-type hOFD1, Ol-TBC1D31 KD + wild-type hOFD1, hOFD1S735A, Ol-TBC1D31 KD + hOFD1S735A, Ol-TBC1D31  KD + hOFD1S735D and Ol-TBC1D31  KD + hOFD1S735D + hpraja2rm stained  with anti-acetylated α-tubulin antibody (green) and DAPI (blue)."
NCBI gene IDs:
"OFD1: 8481
praja2: 9867
TBC1D31: 101172761
TBC1D31: 93594"
Figure legend: "C, D, Currents from IHCs of control (C, P9) and Kir2.1-OE (D, P10) pre-hearing mice. Currents were elicited by using depolarizing and hyperpolarizing voltage steps, with a nominal increment of 10 mV, from a holding potential of −84 mV. Test potentials are shown next to some of the traces. Note that the large inward rectifier Kir2.1 current is only present in the IHC of the Kir2.1-OE mouse (D). The outward current is primarily carried by a delayed rectifier current IK. IK1 identifies the small inwardly rectifying K+ current normally expressed in IHCs. E, Steady-state current-voltage curves obtained from IHCs of control (P9-P11) and Kir2.1-OE (P9-P11) mice."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "F, G, Representative ∆F/F0 traces from the IHCs and GER of P7-P9 control (F) and Kir2.1-OE (G) mice in the presence of 0.3 mM Ca2+. Spontaneous ATP-dependent Ca2+ waves from the GER (green traces) were eliciting coordinated Ca2+ signals in the IHCs from both controls and Kir2.1-OE mice. For each genotype, two separate sets of recordings from 2 mice are shown (top and bottom right), with the top traces being linked to the images on the left: before [1], during [2] and after [3]) the generation of a large Ca2+ wave from the GER. For details about the frequency and duration of the Ca2+ waves, and the number of mice and recordings see Fig EV3. All recordings were obtained at body temperature. Traces are computed as pixel averages of regions of interest centred on IHCs."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "C, Peak-to-peak MET current-voltage curves from P6-P7 apical-coil IHCs of 7 control (12 IHCs) and 3 littermates Kir2.1-OE mice (7 IHCs). Recordings were obtained by mechanically stimulating the hair bundles of IHCs at the same time as stepping their membrane potential from −124 mV to +96 mV in 20 mV increments. The two sets of data are not significantly different: P = 0.6320, 2-way ANOVA."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "C, Peak-to-peak MET current-voltage curves from P8-P9 apical-coil IHCs of 12 control (17 IHCs) and 13 littermates Kir2.1-OE mice (24 IHCs). The two sets of data are significantly different: P &lt; 0.0001, 2-way ANOVA."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "J, Example of FM1-43 uptake by IHCs from P11 control (top) and Kir2.1-OE (bottom) mice, showing the lack of fluorescence labelling in the latter, which is an indication of the lack of MET channels open at rest at this stage in the IHCs overexpressing the Kir2.1 channels. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "A, B, Scanning electron microscope (SEM) images showing the IHC hair bundle structure in the apical coil of the cochlea of P11 control (A) and P8-P11 Kir2.1-OE (B) mice. Control IHCs and the large majority of P8 IHCs from Kir2.1-OE mice show a normal hair bundle structure composed of three rows of stereocilia: tall, intermediate and short (arrows). From about P9 in Kir2.1-OE mice, some IHCs start to lose the third row of stereocilia (arrowheads) and some already exhibit some fusion of the stereocilia (asterisk). These changes in hair-bundle structure became more prominent at P11. At least 3 mice for each genotype were used. In these panels and those below, asterisks are used to define some of the abnormal hair bundles."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "C, D, SEM images of both IHCs and OHCs from the cochlea of P26 control (C, upper panel) and P26 Kir2.1-OE (D, upper panel) mice. Lower panels show a higher-magnification view of the hair bundle of IHCs from both genotypes, highlighting the profound disruption of the stereocilia in IHCs overexpressing Kir2.1 channels. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "A,B, Maximum intensity projections of confocal z-stacks showing images of the hair bundles from apical-coil IHCs of P6 and P11 control (A) and Kir2.1-OE (B) mice immunostained with antibodies against MYOSIN VI (blue) and EPS8 (magenta). At least 3 mice for each genotype were used. In all panels (A-D), stereocilia are labelled with phalloidin (green). Note that despite the disrupted hair-bundle structure in the IHCs overexpressing Kir2.1 channels; the stereocilia retained a normal distribution of these bundle proteins. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "C,D, Confocal images of the hair bundles of P11 IHCs from control (C) and Kir2.1-OE (D) mice immunostained with antibodies against MYOSIN XV isoform 1 (blue) and WHIRLIN (magenta). In all panels (A-D), stereocilia are labelled with phalloidin (green). Note that despite the disrupted hair-bundle structure in the IHCs overexpressing Kir2.1 channels; the stereocilia retained a normal distribution of these bundle proteins. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "A,B, Maximum intensity projections of confocal z-stacks showing the IHCs of the apical cochlear region from control (A) and Kir2.1-OE (B) pups with the females being in the continuous presence of DOX in the drinking water from conception up to when the pups were P5 (upper panels). Middle and bottom panels show IHCs at P7 and P14 following the removal of DOX at P5 for both control (A) and Kir2.1-OE mice (B). IHCs were stained with antibodies against the K+ channel Kir2.1 (green) and Myosin 7a (Myo7a, blue: cell marker). Note that after 2 days following the removal of DOX, Kir2.1overexpression was already largely downregulated. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "H,I, Maximum intensity projections of confocal z-stacks showing images of the hair bundles from apical-coil IHCs of P14 control (H) and Kir2.1-OE (I) mice stained with phalloidin. DOX was removed from the mother's drinking water when the pups were P5. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "J,K, SEM images showing the normal structure of the hair bundles of the IHCs in the apical coil of the cochlea of P14 control (J) and P14 Kir2.1-OE (K) mice. DOX was removed from the mother's drinking water when the pups were P5. Note that the morphological profile of the hair bundles in IHCs is comparable between control and Kir2.1-OE mice, indicating that the removal of the intrinsic Ca2+ action potentials prior to the second postnatal week has no effect on the mechanoelectrical transduction apparatus. At least 3 mice for each genotype were used."
NCBI gene IDs:
"Kir2.1: 16518"
Figure legend: "The 100 stiff ALDH+ or soft ALDH- 4T1 cells were injected into the mammary fat pads of NSG mice. Eight weeks later, the lung sections were H&amp;E stained. metastasis index was calculated (D). Scale bar, 0.5 mm. n = 5 mice with metastatic tumor. Data information: two-tailed Paired Student's t test data represent mean ± SD."
NCBI gene IDs:
"ALDH: 11668"
Figure legend: "(A) The expression of BCL9L or β-catenin in stiff or soft 4T1, MCF-7, B16 or MP-1 cells was determined by western blot. (B) The expression of β-catenin and BCL9L from SGGFP, BCL9L-SGs-4T1, MCF-7, B16 or MP-1 cells were determined by western blot. Data information n = 3 independent experiments in (A and B)."
NCBI gene IDs:
"GFP: 
BCL9L: 80288"