EMBO/SourceData huggingface dataset:
Figure legend: "(f) Immunoblots for LRRK2 and LAMP-2A of homogenates and lysosome-enriched fractions isolated from control fibroblasts (Ctr) or fibroblasts stably interfered for LAMP-2A (L2A(−)), maintained in the presence or absence of serum. Actin and LAMP-1 (L-1) are shown as loading controls of homogenate and lysosomes, respectively. All values are mean + s.e.m.; *P 0.05, unpaired t-test. Full-length blots and gels in Supplementary Figure 12."
NCBI gene IDs:
"LAMP-2A: 16784"
Figure legend: "(a) Top: immunoblot for GFP of tet-on HEK293 cells expressing GFP-tagged WT or G/S LRRK2 pulsed 24 h with doxycycline and, 48 h later (no longer undergoing de novo synthesis), incubated without additions (−) or with 20 mM NH4Cl and 100 μM leupeptin (NL), 5 μM MG132 (MG) or 10 mM 3MA. Bottom: percentage inhibition (n = 3 independent experiments)."
NCBI gene IDs:
"tet: 
LRRK2: 120892"
Figure legend: "(a) Top: immunoblot for GFP of tet-on HEK293 cells expressing GFP-tagged WT or G/S LRRK2 pulsed 24 h with doxycycline and, 48 h later (no longer undergoing de novo synthesis), (b) Same cells as in a, untreated (Ctr) or treated with RNA interference for LAMP-2A (L2A(−)), pulsed as in a, sequentially collected and immunoblotted. Top left panels: representative immunoblots for GFP. Actin is shown as a loading control. Bottom left panels, quantification, corrected by levels of knockdown and loading control (n = 3 or 4 independent experiments). Top right: immunoblot for LAMP-2A. Bottom right: percentage of LRRK2 degraded per hour in the same samples."
NCBI gene IDs:
"tet: 
LAMP-2A: 3920
LRRK2: 120892"
Figure legend: "(c) Binding, association and uptake calculated from quantification of LRRK2 in immunoblots of lysosomes from starved rat livers, untreated or pretreated with protease inhibitors (PI) and incubated with WT or G/S LRRK2. Inset: representative immunoblot (n = 9 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(d) Association of GAPDH (5 μg) to starved rat liver lysosomes in the presence of increasing concentrations of WT or G/S LRRK2. Inset: representative immunoblot n = 4-6."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(e,f) Binding of WT and G/S LRRK2 to lysosomes in the presence of GAPDH (e) or RNase A (f). Top: representative immunoblots. Bottom: lysosome-bound LRRK2 expressed as a multiple of the amount bound when added alone. Trends of mean values (n = 2 independent experiments); error bars indicate range."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(h,i) Rat brain lysosomes incubated as in d and e, respectively. Left: representative immunoblots. Right: quantifications (n = 3 or 4 independent experiments). All values expressed as mean + s.e.m.; differences with untreated samples (*) and between WT LRRK2 and G/S LRRK2 (§) were significant at P 0.05; n.s., not significant; ANOVA and Bonferroni test. Full-length blots and gels in Supplementary Figure 12."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(a) Proteolysis in HEK293 cells stably transfected with an empty plasmid (Ctr) or plasmids expressing WT or G/S LRRK2 under the control of a tet promoter, treated with doxycycline to activate protein expression and then labeled with [3H]leucine for 48 h. After extensive washing, protein degradation at the indicated times was measured, in cells maintained either in the presence (+) or absence (-) of serum, as the amount of acid-precipitable radioactivity (amino acids and small peptides) released into the media. Values are expressed as percentage of radioactivity in proteins (acid precipitable) at time 0 (n = 4 independent experiments, each in triplicate)."
NCBI gene IDs:
"tet: 
LRRK2: 120892"
Figure legend: "(c) Cultured ventral midbrain dopaminergic neurons from Ctr and from WT-LRRK2 and G/S-LRRK2 transgenic mice, transduced with the same reporter as in b and co-stained with antibody to tyrosine hydroxylase (TH). Nuclei are highlighted with DAPI (4′,6-diamidino-2-phenylindole) in blue. Left: representative images. Right: average number of puncta per cell in &gt;50 TH-positive cells. Scale bars, 10 μm. All values are mean + s.e.m.; differences with Ctr (*) and between WT LRRK2 and G/S LRRK2 (§) were significant at P 0.05; unpaired t-test in a and ANOVA and Bonferroni test in b,c."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(a) Immunoblots of rat liver lysosomes incubated with WT or G/S LRRK2 alone or with 1 mM GTP and/or GAPDH. Duplicate samples are shown. Bottom: quantification of lysosome-bound LRRK2 expressed as a multiple of the amount bound without additions (n = 6 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(b) Effect of kinase inhibitor on WT, G/S, or the kinase-dead mutant D/A LRRK2 incubated with rat liver lysosomes in presence or absence of GAPDH (5 μg). Right: quantification expressed as a multiple of the association without additions (n = 3-5 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(c,d) Transient transfection of HEK293 cells with the myc- or GFP-tagged LRRK2 constructs indicated at top left in c. Full, full length; M, KFERQ-like motifs (M1-M8). (c) Binding to GST-hsc70. Left, representative immunoblots; input lanes, one-tenth of input material. NT, non-transfected cells. Immunoblot for hsc70 is shown as loading control. Top right: quantification of LRRK2 bound to hsc70. Where indicated, samples were also incubated with RNase A to compete the KFERQ-mediated binding of hsc70 (n = 3 or 4 independent experiments). (d) Coimmunoprecipitation (IP) of hsc70 with anti-myc or anti-GFP in the same cells. Inputs (Inp), one-quarter of starting volume."
NCBI gene IDs:
"hsc70: 3312
LRRK2: 120892"
Figure legend: "(f) Co-IP of LAMP-2A with anti-LRRK2 in lysosomes incubated with WT or G/S LRRK2. Inp, one-quarter of starting sample; IgG, immunoglobulin G. Values: LAMP-2A recovered in the IP (IP L2A) corrected for the amount of LRRK2 pulled down and expressed as a multiple of WT value."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(g,h) Duplicate LAMP-2A immunoblots of rat liver lysosomes incubated alone (−) or with WT or G/S LRRK2 (g) or with lysosomes from control (Ctr) or from WT-LRRK2 or G/S-LRRK2 transgenic mice (h) and subjected to blue native electrophoresis. Arrow, 700-kDa multimeric complex. All values are mean + s.e.m.; differences compared to none or untreated (*) or to WT or full-length protein (§) were significant at P 0.05; unpaired t-test in a and ANOVA and Bonferroni test in b,c. Full-length blots and gels in Supplementary Figure 12."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(a) Top: LRRK2 immunoblot of rat liver lysosomes incubated with WT, G/S, D/A (kinase-dead), I/T or R/C mutant LRRK2, alone or in the presence of protease inhibitors (PI). Input lanes, one-quarter of input material. Bottom: quantification of binding and uptake (calculated as in Fig. 2b; n = 4 or 5 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(c) Top: coimmunoprecipitation (co-IP) of LAMP-2A (L2A) with anti-LRRK2 from lysosomes incubated with LRRK2proteins as in a. Co-immunoprecipitated fractions were immunoblotted for LAMP-2A and LRRK2. Input: immunoblot for LAMP-2A before the affinity purification. Bottom: amount of LAMP-2A coimmunoprecipitated, expressed as a multiple of the value with WT LRRK2 (n = 3 or 4 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(d) Co-IP of LAMP-2A with anti-GFP in tet-on HEK293 cells expressing GFP-LRRK2 proteins and pulsed for 24 h with doxycycline. Inputs (Inp), one-quarter of material added."
NCBI gene IDs:
"tet: "
Figure legend: "(a) α-syn immunoblot of rat liver lysosomes incubated with WT or mutant (A53T) α-syn alone (−) or with WT LRRK2 or G/S LRRK2. Inp, input; Mon, monomers. Right: quantification of α-syn oligomers, expressed as a multiple of amounts in samples without LRRK2 (n = 4 or 5 independent experiments)."
NCBI gene IDs:
"LRRK2: 120892
α-syn: 6622"
Figure legend: "(e) Ventral midbraindopaminergic neuronal cultures from control (Ctr) or WT-LRRK2 or G/S-LRRK2 transgenic mice untreated or treated with RNAi for LAMP-2A (L2A(−)). (f) Colocalization between α-syn and total LAMP-2 from cells in e (n &gt; 50 tyrosine hydroxylase (TH)-positive neurons). Total LAMP-2 is not reduced in L2A knockdown cells because LAMP-2B is upregulated."
NCBI gene IDs:
"LAMP-2A: 16784
LRRK2: 120892"
Figure legend: "(i,j) iPSC lines as in g, differentiated for 30 or 75 d and untreated or treated with RNAi for LAMP-2A. (i) Immunofluorescence for LAMP-2A, α-syn and GFP in LAMP-2A-RNAi iPSC lines differentiated for 30 d. Bottom: higher magnifications of boxed areas. (j) Percentage of TH+ cells showing cytoplasmic accumulation of α-syn. n ≥ 2 experiments using two control and two LRRK2 iPSC lines (n &gt; 70 and n &gt; 150 TH+ cells in Ctr and LRRK2, respectively). Scale bars, 10 μm. All values are mean + s.e.m. Differences from WT or control (*) or between control or knockdown cells (§) are significant at P 0.05; unpaired t-test in a,h and ANOVA and Bonferroni test in f,j. Full-length blots and gels in Supplementary Figure 12."
NCBI gene IDs:
"LAMP-2A: 3920"
Figure legend: "(A-C) Excision of endogenous Hi in cmt23 (A), drm12 (B), and ddcc (C) mutant backgrounds when HiTG is segregated away. Plants with * and ** were used for WGBS, and the results of those with ** were used for the analysis shown in Figures."
NCBI gene IDs:
"cmt2: 827640
drm1: 831390"
Figure legend: "(D) Excision of endogenous Hi in mutant backgrounds of epigenetic regulators. drm12: drm1 drm2 double mutant, ddc: drm1 drm2 cmt3 triple mutant, cmt23: cmt2 cmt3 double mutant, and ddcc: drm1 drm2 cmt2 cmt3 quadruple mutant. Histone acetyltransferase HDA6/RTS1 is reported as important for silencing of TEs, and TEs activated in hda6 mutant are shared with those activated in met1"
NCBI gene IDs:
"cmt2: 827640
cmt3: 843313
drm1: 831390
drm2: 831315
hda6: 836431
HDA6: 836431
RTS1: 836431
met1: 834975"
Figure legend: "(A-C) Patterns of DNA methylation in endogenous Hi (A), AT2TE05755 (B), and AT2TE06955 (C) in the drm12 mutant background. '(-HiTG)' indicates that HiTG was segregated. Vertical dashed lines indicate termini of TEs."
NCBI gene IDs:
"AT2TE05755: 3768055
AT2TE06955: 814971
drm1: 831390"
Figure legend: "(A) Patterns of DNA methylation in flanking regions of VANC21 targets when HiTG was segregated in drm12 (left) and cmt23 (right) mutant background. Grey dashed lines indicate the points of VANC targets."
NCBI gene IDs:
"VANC21: 816880
cmt2: 827640
drm1: 831390"
Figure legend: "(C) Patterns of DNA methylation around VANC21 (left), VANC6 (middle), and VANC1 (right) targets."
NCBI gene IDs:
"VANC1: 841076
VANC21: 816880
VANC6: 3769791"
Figure legend: "(C) Hi excision in F2 plants obtained from met1 x GK345. Genotyping results of MET1 and Hi loci are also shown. a-h indicate individuals who are analyzed for their DNA methylation status in (D)."
NCBI gene IDs:
"met1: 834975
MET1: 834975"
Figure legend: "(A) Expression of VANB21 (At2g23490) and VANC21 (At2g23480) measured by RT‒qPCR. The values were normalized to the expression levels in the ddm1 mutant. For each genotype, the average values and standard deviations of the three technical replicates are shown. Note that we could not detect the expression of VANC21 in drm12 (-HiTG), although endogenous Hi retained mobility (Fig. 6C). This finding can likely be explained by the expression of VANA21, a transposase of VANDAL21, but we failed to compare the expression levels of this gene because of its very low expression level."
NCBI gene IDs:
"VANC21: 816880
At2g23480: 816880
VANA21: 816880
VANDAL21: 816880
VANB21: 816881
At2g23490: 816881
ddm1: 836808
drm1: 831390"
Figure legend: "(B) DNA methylation status of coding and noncoding regions of five VANDAL21 TEs The average values and standard deviations for each region are shown. One-way ANOVA with Tukey's multiple comparisons test was used to determine the significant differences (p&lt;0.01)."
NCBI gene IDs:
"VANDAL21: 816880"
Figure legend: "(A) Immunoblot analysis of caspase-1 and gasdermin D in WT or Nlrp3-/- unprimed and LPS-primed BMDMs left untreated [Medium alone (Med.)] or assessed 20 hr after stimulation with the supernatant (Sup.) of or assessed 20 hr after infected with WT C. perfringens (WT C. per), ΔpfoA C. perfringens (ΔpfoA), Δcpa C. perfringens (Δcpa, lacking lecithinase) or ΔpfoAΔcpa C. perfringens (ΔpfoAΔcpa) at MOI 100."
NCBI gene IDs:
"cpa: 69447757
lecithinase: 69447757
Nlrp3: 216799
pfoA: 69447922"
Figure legend: "(A) Concentration of IL-18 in the serum of WT mice, 8 hr after intraperitoneal infection with 4 × 108 colony-forming units (CFUs) of C. perfringens (WT C. per, n = 14), ΔpfoA C. perfringens (ΔpfoA, n = 13), Δcpa C. perfringens (Δcpa, n = 11) or ΔpfoAΔcpa C. perfringens (ΔpfoAΔcpa, n = 12). (B) Concentration of IL-18 in the peritoneal fluid of WT mice, 4 hr after intraperitoneal infection with 4 × 108 CFUs of C. perfringens (WT C. per, n = 14), ΔpfoA C. perfringens (ΔpfoA, n = 15), Δcpa C. perfringens (Δcpa, n = 17) or ΔpfoAΔcpa C. perfringens (ΔpfoAΔcpa, n = 14). (C) Concentration of IL-18 in the serum of WT mice (n = 10), Nlrp3-/- mice (n = 9), and Casp1/11-/- mice (n = 19), 6 hr after intraperitoneal infection with 1 × 108 CFUs of C. perfringens. (D) Concentration of IL-18 in the peritoneal fluid of WT mice (n = 10), Nlrp3-/- mice (n = 9), and Casp1/11-/- mice (n = 19), 3 hr after intraperitoneal infection as described in C."
NCBI gene IDs:
"Casp1: 12362
cpa: 69447757
Nlrp3: 216799
pfoA: 69447922"
Figure legend: "(G) Survival of WT (n = 19), Nlrp3-/- (n = 26) and Casp1-/- (n = 10) mice, after intraperitoneal administration of 0.312 Units/ml of lecithinase (Lec.)."
NCBI gene IDs:
"Casp1: 12362
Nlrp3: 216799"
Figure legend: "(I) Release of IL-1β (top), IL-18 (middle) and death (bottom) of WT or Ninj1-/- BMDMs left untreated or LPS-primed and assessed for a range of time points after stimulation with lecithinase (Lec.) or 16 hr after transfection with LPS (LPS trans.)."
NCBI gene IDs:
"Ninj1: 18081"
Figure legend: "(J) Immunoblot analysis of caspase-1, gasdermin D and NINJ1 of WT or Ninj1-/- BMDMs left untreated or LPS-primed and assessed 3 hr after stimulation with lecithinase (Lec.) or 16 hr after transfection with LPS (LPS trans.)."
NCBI gene IDs:
"Ninj1: 18081"
Figure legend: "H Western blot  confirming no YAP  and TAZ  expression in two independent (#1 and #2) YAP/TAZ  double knockout (Y/T DKO) clones."
NCBI gene IDs:
"TAZ: 6901
YAP: 10413"
Figure legend: "I Relative expression  levels of YAP/TAZ  target genes CYR61  and CTGF  in HEK293A  WT and Y/T DKO clones in response to cyclic 0.1Hz 200mbar hydrostatic pressure. Data from six independent experiments Error bars represent mean ± SD. Kruskal-Wallis test with Dunn's post hoc. **p=0.0661 (WT CYR61), ***p= 0.0001 (WT CTGF), p=0.3924 (Y/T DKO#1 CYR61), p=0.4616 (Y/T DKO#1 CTGF), p&gt;0.999 (Y/T DKO#2 CYR61), p=0.0782 (Y/T DKO#2 CTGF)."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1490
CTGF: 1265
TAZ: 6901
YAP: 10413"
Figure legend: "J CYR61  and CTGF gene expression levels induced by  hydrostatic pressure in (I) of Y/T DKO #1 and #2 normalised to WT. Kruskal-Wallis test with Dunn's post hoc. *p=0.0259 (Y/T DKO#1 CYR61), **p=0.0007 (Y/T DKO#1 CTGF), *p=0.0155 (Y/T DKO#2 CYR61), ***p&lt;0.001 (Y/T DKO#2 CTGF)."
NCBI gene IDs:
"CYR61: 3491
CTGF: 1265"
Figure legend: "B Comparison of cell volume  of WT and  LATS1/2 DKO clones at steady state obtained using DHM. Each dot represents a single cell. Data from three independent experiments. Error bars represent mean ± 95% CI. Kruskal-Wallis test with Dunn's post-hoc. **p=0.0018 (WT vs LATS1/2 DKO #1), ***p&lt;0.001 (WT vs LATS1/2 DKO #2), p=0.0557 (LATS1/2 DKO #1 vs #2)."
NCBI gene IDs:
"LATS1: 9113"
Figure legend: "C Comparison of cell volume  of WT and MST1/2 DKO at steady state obtained using DHM. Each dot represents a single cell. Data from three independent experiments. Error bars represent mean ± 95% CI. Mann-Whitney U test. p=0.2731."
NCBI gene IDs:
"MST1: 4485"
Figure legend: "D HEK293A NF2  KO cells response to 100mbar cyclic hydrostatic pressure. Each dot represents a single cell and Error bars represent mean ± 95% CI. Data pooled from three independent experiments. Mann-Whitney U test. p=0.4072."
NCBI gene IDs:
"NF2: 4771"
Figure legend: "E Comparison of cell volume of WT and TEAD  KO clones at steady state obtained using DHM. Each dot represents a single cell. Data from three independent experiments. Error bars represent mean ± 95% CI. Kruskal-Wallis test with Dunn's post-hoc. p&gt;0.9999 for all comparisons."
NCBI gene IDs:
"TEAD: 7004///7005///8463///7003"
Figure legend: "K Representative immunofluorescence  images showing MYC-tagged YAP  expression in WT YAP  re-expressing cells and S94A mutant YAP  re-expressing cells compared to Y/T DKO vector control. 95% were positive for expression of YAP  or YAP  mutant. Scale bar = 20µm."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "L  Western blot showing total YAP, TAZ  and CYR61  levels in WT YAP  and S94A mutant YAP  re-expressing cells compared to WT and Y/T DKO vector control."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "M Average change in cell volume in response to  hydrostatic pressure in WT YAP  and S94A mutant YAP  re-expressing cells relative to Y/T DKO. Each dot represents a single cell. Error bars represent mean ± 95% CI. Data pooled from three independent experiments. Kruskal-Wallis test with Dunn's post hoc. p&lt;0.001 (vector vs. WT YAP), p=0.4912 (vector vs. S94A YAP), p=0.0327"
NCBI gene IDs:
"YAP: 10413"
Figure legend: "N Average change in cell volume in response to 100mbar cyclic  hydrostatic pressure  in WT and TEAD  KO clones. Each dot represents a single cell. Data pooled from three independent experiments. Error bars represent mean ± 95% CI. Kruskal-Wallis test with Dunn's post hoc. ***p &lt; 0.001 (for both comparisons), p&gt;0.9999 (TEAD  KO #1 vs #2)."
NCBI gene IDs:
"TEAD: 7003///8463///7005///7004"
Figure legend: "C Representative immunofluorescence  images showing Phalloidin-labelled actin  (red) and Hoechst  (blue) in Latrunculin B (LatB) (0.5µM) or Cytochalasin D (CytD) (2µM)-treated HEK293A  cells compared to control across Hippo pathway genome-edited cells as shown. Scale bar = 20μm. D Average change in  cell volume obtained by DHM  in response to hydrostatic pressure with 2µM Cytochalasin D  (CytD) treatment in WT, Y/T DKO and LATS1/2 DKO cells. Each dot represents a single cell and error bars represent mean ± 95% CI. Data pooled from four independent experiments. Kruskal-Wallis test with Dunn's post hoc. **p=0.0044 (WT con vs CytD), p*=0.0409 (Y/T DKO con vs CytD), p&gt;0.9999 (LATS1/2 DKO con vs CytD), ***p=0.0003 (WT con vs Y/T DKO con), ***p&lt;0.001 (WT con vs Y/T DKO CytD), ***p&lt;0.001 (Y/T DKO CytD  vs LATS1/2 DKO CytD). E Fold difference in average change in  cell volume in response to dynamic hydrostatic pressure from (H) normalised against untreated cells. Each dot represents a single cell and error bars are 95% CI. Mann-Whitney U test. **p=0.0069 (WT vs. Y/T DKO), **p=0.0031 (WT vs LATS1/2 DKO). F Average change in cell volume in response to hydrostatic pressure with 0.5µM  Latrunculin B (Lat B) treatment in WT, Y/T DKO and LATS1/2 DKO cells. Each dot represents a single cell and error bars represent mean ± 95% CI. Data pooled from four independent experiments. Kruskal-Wallis test with Dunn's post hoc. ***p&lt;0.001 (all comparisons), p=0.0972 (LATS/12 DKO con vs LatB), *p=0.0216 (Y/T DKO LatB  vs LATS1/2 DKO LatB). G Fold difference in average change in cell volume in response to cyclic 0.1Hz 100mbar  hydrostatic pressure from (F) normalised against control. Each dot represents a single cell and error bars represent mean ± 95% CI. Mann-Whitney U test. p=0.1472 (WT vs. Y/T DKO), **p=0.0015 (WT vs LATS1/2 DKO)."
NCBI gene IDs:
"LATS1: 9113"
Figure legend: "A Confocal IF  images labelled with Hoechst  (Blue) and internalised Transferrin   (Trf). Cells were allowed to internalize  for ten minutes, fixed and acid stripped to remove surface bound Trf. Hydrostatic pressure (H.P.) promotes transferrin  uptake in WT and LATS1/2 DKO cells. Scale bar = 20µm. B Quantification of transferrin  uptake in WT, Y/T DKO and LATS1/2 DKO cells treated with  hydrostatic pressure  compared to steady state. Each dot represents a single cell and error bars represent mean ± 95% CI. Data from four independent experiments. Kruskal-Wallis test with Dunn's post hoc. Steady-state statistical analysis is shown in blue, transferrin  uptake induced by pressure is shown in black. ***p&lt;0.001 (WT con vs +H.P.), p=0.2375 (Y/T DKO con vs +H.P.), ***p&lt;0.001 (LATS1/2 DKO con vs +H.P.), p=0.2764 (WT con vs Y/T DKO con), p=0.0808 (WT con vs LATS1/2 DKO con), ***p=0.0004 (Y/T DKO con vs LATS1/2 DKO con)."
NCBI gene IDs:
"LATS1: 9113"
Figure legend: "C Confocal IF   images myc  tagged AP180C   (green) cells showing internalised Trf  (red) and stained  with Hoechst (blue). AP180C  inhibits transferrin uptake in WT and Y/T DKO cells. Scale bar = 20µm. D Quantification of transferrin  uptake in AP180C  positive cells compared to vector control from images as in C. Each dot represents a single cell from four independent experiments and error bars represent mean ± 95% CI. Mann-Whitney U test. ***p &lt; 0.001 (for both comparisons)."
NCBI gene IDs:
"AP180C: "
Figure legend: "E Confocal IF  images of HEK293A WT cells showing subcellular localisation  of YAP  (red) in AP180C  (green) negative and positive WT and LATS1/2 DKO cells. Cells also stained  with Hoechst (blue). F Quantification of cytoplasmic-to-nuclear  ratio of YAP  in AP180C  positive WT and LATS1/2 DKO cells compared to vector control from images as in E. Each dot represents a single cell pooled from four independent experiments. Error bars represent mean ± 95% CI. Mann-Whitney U test. ***p=0.0008 (WT), p=0.9318 (LATS1/2 DKO)."
NCBI gene IDs:
"LATS1: 9113"
Figure legend: "(A) Morphological phenotypes of Wild type (WT), mkk1 mkk2 and summ3-1 mkk1 mkk2 plants. Photos were taken on three-week-old soil-grown plants."
NCBI gene IDs:
"summ3: 815625
mkk1: 828713
mkk2: 829103"
Figure legend: "(D-E) Expression of PR1 (D) and PR2 (E) in the indicated genotypes as determined by quantitative RT-PCR. Values were normalized to the expression levels of ACTIN1. The data are shown as mean ± SD (n=3) with one-way ANOVA analysis and Tukey test (P &lt; 0.01). Different letters indicate significant differences. The experiments were repeated three times with similar results."
NCBI gene IDs:
"ACTIN1: 
PR2: 824893
PR1: 815949"
Figure legend: "(A) Morphological phenotypes of WT, summ3-17, mekk1-1 and summ3-17 mekk1-1 plants grown on soil for three weeks."
NCBI gene IDs:
"summ3: 815625
mekk1: 826409"
Figure legend: "(B-C) Expression levels of PR1 (B) and PR2 (C) in the indicated genotypes as determined by quantitative RT-PCR. Values were normalized to the expression levels of ACTIN1. The data are shown as mean ± SD (n=3) with one-way ANOVA analysis and Tukey test (P &lt; 0.01). Different letters indicate significant differences. The experiments were repeated three times with similar results. Two-week-old seedlings grown on half MS plates were used for assays in (B)-(C)."
NCBI gene IDs:
"ACTIN1: 
PR2: 824893
PR1: 815949"
Figure legend: "(D) Morphological phenotypes of WT, mpk4-3, summ3-1 mpk4-3 and summ3-2 mpk4-3 plants. Photos were taken on three-week-old soil grown plants."
NCBI gene IDs:
"summ3: 815625
mpk4: 828151"
Figure legend: "(E-F) Expression levels of PR1 (E) and PR2 (F) in the indicated genotypes as determined by quantitative RT-PCR. Values were normalized to the expression levels of ACTIN1. The data are shown as mean ± SD (n=3) with one-way ANOVA analysis and Tukey test (P &lt; 0.01). Different letters indicate significant differences. The experiments were repeated three times with similar results. Two-week-old seedlings grown on half MS plates were used for assays in (E)-(F)."
NCBI gene IDs:
"ACTIN1: 
PR2: 824893
PR1: 815949"