EMBO/SourceData huggingface dataset:
Figure legend: "HEK293 cells were transfected with WT α11.2, α2δ-1, and β2A + WT α-actinin-1 and cultured for 22-24h before surface biotinylation. Shown are representative immunoblots of NeutrAvidin pull-down samples (from lysate containing 600 μg protein) and total lysate samples containing 20 μg protein. α11.2 was detected with an antibody against its HA tag (top), which is present in all constructs used throughout this work. Pull-down and lysate samples are from the same blot but different exposures because signals from lysate samples were much stronger than from pull-down samples. Immunoblotting for vinculin (middle) and tubulin (bottom) indicated that comparable amounts of these intracellular control proteins were present in lysate samples. Their absence in pull-down samples as seen on the same blots showed that these prominent intracellular proteins did not undergo biotinylation as control for membrane integrity during surface biotinylation. Bar graph shows means + SEM of the pull-down immunosignals in mutants relative to surface labelling of control α11.2 samples lacking α-actinin-1 co-expression (mean set to 100%; see Methods; **p&lt;0.01 two-tailed t-test; n = 7)."
NCBI gene IDs:
"α-actinin-1: 87
α11.2: 24239
α2δ-1: 100009105
β2A: 116600"
Figure legend: "HEK293 cells were transfected with WT or K1647E mutant α11.2, α2δ-1, and β2A, and WT or E847K/E851K mutant α-actinin-1, or CFP alone as negative control and cultured for 22-24h before surface biotinylation. Shown are representative immunoblots of pull-down and lysate samples as in (a). Bar graph shows means + SEM of the pull-down immunosignals in mutants relative to surface labelling in the WT/WT control (mean set to 100%; see Methods). **p&lt;0.01, ***p &lt; 0.001; one-way ANOVA with Tukey post hoc test; n = 4-5)."
NCBI gene IDs:
"CFP: 
α-actinin-1: 87
α11.2: 24239
α2δ-1: 100009105
β2A: 116600"
Figure legend: "HEK293 cells were transfected with α11.2, α2δ-1, and β2A before whole cell patch recording in 20 mM Ba2+. Representative current traces of the first 2 ms obtained from recordings upon depolarizations from a holding potential of -80 mV to the indicated potentials (the voltage protocol is schematized in the upper left corner). Representative current traces upon step depolarizations to the reversal potential (Erev) for 20 ms to obtain movement of the ON-gating charges (Qon), and subsequent to -50 mV for 10 ms to obtain tail currents (Itail). Insets: magnifications of exemplary Qon for CaV1.2 WT, K1647A, Y1649A, I1654A, F1658A and K1662E. Plots of Itail (in this panel corrected for variations in cell capacitance) versus total detectable charge transfer for Qon. Slopes of regression curves are strongly reduced for CaV1.2 K1647A, Y1649A and I1654A versus WT (see Table 7 for more details). Dot plots and means + SEM of Qon (in this panel corrected for variations in cell capacitance; *p&lt;0.05; **p&lt;0.01; one-way ANOVA with Bonferroni post-hoc test; n = 13-18; see Table 7 for more details). The reduction in slope of the regression curve of combined population data for K1647A, Y1649A and I1654A versus WT indicates reduced coupling of Itail with Qon when α-actinin binding to the IQ motif is diminished."
NCBI gene IDs:
"α11.2: 24239
α2δ-1: 100009105
β2A: 116600"
Figure legend: "(D) IF of DAXX and ATRX in PN5 zygotes upon siRNA-mediated knockdown of Daxx (n=14) or with control siRNAs (n=8). Data information: All panels: images of paternal and maternal pronuclei at given stage/condition are from same zygote. Yellow dashed circles represent the contours of the pronuclei. All scale bars, 10µm."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(E) IF of DAXX and ATRX in control (n=15) and Atrxm-z+ (n=14) PN5 zygotes. Data information: All panels: images of paternal and maternal pronuclei at given stage/condition are from same zygote. Yellow dashed circles represent the contours of the pronuclei. All scale bars, 10µm."
NCBI gene IDs:
"Atrx: 22589"
Figure legend: "(A) IF of DAXX and EZH2 in paternal pronuclei of Ezh1m-z+ (n=5), Ezh2m-z+ (n=3) and Ezh1m-z+; Ezh2m-z+ (n=4) late zygotes. Yellow dashed circles represent the contours of the pronuclei. Data information: All scale bars, 10µm."
NCBI gene IDs:
"Ezh1: 14055
Ezh2: 14056"
Figure legend: "(C) IF of DAXX in wild type (n=30), Ring1m-z+ (n=12), Rnf2m-z+ (n=11) and Ring1m-z+; Rnf2m-z+ (n=12) paternal pronuclei of PN5 zygotes. Data information: All scale bars, 10µm."
NCBI gene IDs:
"Ring1: 19763
Rnf2: 19821"
Figure legend: "(C) Occupancy (% of IP-ed DNA / input DNA) of GAL4DBDCBX2, RNF2, DAXXMycHis, H3.3, and H3 proteins at 5×UAS site as measured by ChIP-qPCR of chromatin from HEK2935xUAS cells. Data are means ± SEM (n = 3)."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
DAXX: 13163
GAL4: 855828
H3.3: 3020///3021
H3: 440093///440686///391769///340096///3020///8290///3021
RNF2: 6045"
Figure legend: "(E) ChIP-seq analysis of HEK2935xUAS cells expressing full length GAL4DBDCBX2.1 and DaxxMycHis showing CBX2.1 dependent recruitment of DAXX to promoters of CCND2 and RUNX1 genes."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
CCND2: 894
Daxx: 13163
DAXX: 13163
GAL4: 855828
RUNX1: 861"
Figure legend: "(F) ChIP-seq analysis of GAL4DBDCBX2.1 occupancy in HEK2935xUAS cells expressing DAXXMycHis (X-axis) or GAL4DBDCBX2.1 and DAXXMycHis (Y-axis), showing (log2) enrichment for GAL4DBDCBX2.1 at many TSSs (± 1 kb) in cells expressing the CBX2.1 protein (Y-axis)."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
DAXX: 13163
GAL4: 855828"
Figure legend: "(G) ChIP-seq analysis of GAL4DBDCBX2.1 versus DAXXMycHis occupancy in HEK2935xUAS cells expressing GAL4DBDCBX2.1 and DAXXMycHis, revealing very comparable enrichment levels (log2) of both proteins at TSSs. TSSs highlighted in red show enrichment with anti-Myc antibody independently of GAL4DBDCBX2.1 occupancy."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
DAXX: 13163
GAL4: 855828"
Figure legend: "(H) ChIP-seq analysis of DAXXMycHis occupancy in HEK2935xUAS cells expressing DAXXMycHis (X-axis) versus GAL4DBDCBX2.1 and DAXXMycHis (Y-axis) showing strong enrichment (log2) for DAXXMycHis at many TSSs of genes, but only when both proteins are expressed (Y-axis), indicating that CBX2.1 targets DAXX to chromatin. TSSs highlighted in red show enrichment with anti-Myc antibody independently of GAL4DBDCBX2.1 occupancy."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
DAXX: 13163
GAL4: 855828"
Figure legend: "(B) ChIP-qPCR analyses of HEK2935xUAS cells co-expressing GAL4DBDCBX2.1 either with DAXXMycHis or DAXXΔSIM1/2MycHis. Data are means ± SEM (n = 3)."
NCBI gene IDs:
"His: 
Myc: 
CBX2: 84733
DAXX: 13163
GAL4: 855828"
Figure legend: "(C) IF of EGFP and DAXX in Daxxm-z+ PN5 zygotes micro-injected with Daxx-EGFP or DaxxΔSIM1/2-EGFP mRNA (100ng/µl). Data information: Scale bars, 10µm."
NCBI gene IDs:
"EGFP: 
Daxx: 13163"
Figure legend: "(D) Left: Boxplot displaying EGFP signal intensities at PCH relative to euchromatin in Daxxm-z+ zygotes micro-injected with Daxx-EGFP or DaxxΔSIM1/2-EGFP mRNA. Right: Boxplot displaying signal intensity of EGFP at euchromatin in same samples. In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 3 times. ***P&lt;0.001, N.S.: no statistical difference, P&gt;0.05, t-test."
NCBI gene IDs:
"EGFP: 
Daxx: 13163"
Figure legend: "(F) Ni-pulldown of 6xHisSUMO1/2/3-conjugated proteins from HEK293 extracts expressing 6xHis-tagged SUMO1, SUMO2 or SUMO3 in combination with CBX2-EGFP. CBX2 and SUMO-conjugated CBX2 were detected with an anti-GFP antibody."
NCBI gene IDs:
"EGFP: 
His: 
CBX2: 84733
SUMO1: 7341
SUMO2: 6613
SUMO3: 6612"
Figure legend: "(G) Ni-pulldown of 6xHis-SUMO2 conjugated proteins from HEK293 extracts transiently co-expressing 6xHis-SUMO2 with CBX2-EGFP or CBX24KR-EGFP. Detection of CBX2"
NCBI gene IDs:
"EGFP: 
His: 
CBX2: 12416
SUMO2: 6613"
Figure legend: "(H) IF of DAXX and EGFP in wild-type PN5 zygotes, micro-injected with Cbx2 or Cbx24KR-EGFP mRNA (100ng/µl). Data information: Scale bars, 10µm."
NCBI gene IDs:
"EGFP: 
Cbx2: 12416"
Figure legend: "(I) Left: Boxplot displaying signal intensity of DAXX at PCH relative to mean intensity at pat-PCH in wild-type zygotes micro-injected with Cbx2 or Cbx24KR-EGFP mRNA. Right: Absolute intensities of EGFP in same samples. In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 2 times. ***P&lt;0.001, t-test."
NCBI gene IDs:
"EGFP: 
Cbx2: 12416"
Figure legend: "(A) IF of DAXX and ATRX in PN5 stage pronuclei of wild-type (n=19), Hp1βm-z+ (n=10) and Suv39h2m-z+ (n=9) zygotes. Data information: Panels Paternal and maternal pronuclei of a given condition originate from same zygotes. All scale bars 10µm."
NCBI gene IDs:
"Hp1β: 12412
Suv39h2: 64707"
Figure legend: "(B) Boxplots displaying signal intensities of DAXX or ATRX at pat- and mat-PCH in wild-type (n=5) and Hp1βm-z+ (n=10) mouse zygotes at PN5. In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 2 times. *P&lt;0.05, **P&lt;0.01, t-test."
NCBI gene IDs:
"Hp1β: 12412"
Figure legend: "(C) IF of DAXX in PN5 stage pronuclei of zygotes expressing CBX2-EGFP (n=9) or CBX2F12A-EGFP (n=17). mRNAs (100ng/µl) were injected into mouse PN2 stage zygotes. Yellow dashed circles represent the contours of the pronuclei.. Data information: Panels C: Paternal and maternal pronuclei of a given condition originate from same zygotes. All scale bars 10µm."
NCBI gene IDs:
"EGFP: 
CBX2: 84733"
Figure legend: "(A) Developmental progression of Daxxm+z+, Daxxm-z+ and Daxxm-z- early embryos."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(D) Still images of time-lapse imaging of the first cleavage division in wild-type and maternally deficient zygotes expressing H2B-mCherry. Time points represent minutes after prometaphase. Lagging chromosomes and micronuclei are indicated by yellow arrowheads. Data information: All scale bars 10µm."
NCBI gene IDs:
"H2B: 
mCherry: "
Figure legend: "(F) IF of 5-methyl and 5-hydroxy-methyl cytosine staining of wild-type (n=42), Daxxm-z+ (n=14) and Ring1m-z+; Rnf2m-z+ (n=6) zygotes with lagging chromosomes. Yellow arrowheads indicate lagging chromosomes. Data information: All scale bars 10µm."
NCBI gene IDs:
"Daxx: 13163
Ring1: 19763
Rnf2: 19821"
Figure legend: "(G) DNA-FISH for major (green) and minor (red) satellites on cleavage chromosomes of a Daxxm-z+ zygote with a chromosome broken within the major satellite region. Data information: All scale bars 10µm."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(H) H3.3-EGFP live-imaging signal in pat- and mat-PCH of control Daxxm+z+ (n=18), Daxxm-z+ (n=8) and Ring1m-z+; Rnf2m-z+ (n=12) zygotes. Scale bar 10µm."
NCBI gene IDs:
"Daxx: 13163
Ring1: 19763
Rnf2: 19821"
Figure legend: "(I) Left: IF of endogenous H3 and ATRX at pat-PCH in control and Daxxm-z+ PN5 zygotes. Right: Fluorescence intensity profiles of H3 and ATRX along white line in left panel at pat-PCH of zygotes. Scale bars 5 µm. Data information: All scale bars 10µm."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(J) Boxplot displaying H3 signal intensity at pat-PCH of Daxxm+z+ and Daxxm-z+ PN5 zygotes. In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 2 times. ***P&lt;0.001, t-test, n=12 for both groups."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(K) IP of DAXX-EGFP and DAXXR244A-EGFP proteins with an anti-GFP antibody from nuclear extract of HEK293 cells. Immuno-blot detection was performed with antibodies recognizing GFP, histone variant H3.3 and CBX2."
NCBI gene IDs:
"EGFP: 
DAXX: 13163"
Figure legend: "(L) Percentage of Daxxm-z+ zygotes with lagging chromosomes following injection of Tubulin-EGFP, Daxx-EGFP or DaxxR244A-EGFP mRNAs (20 or 100 ng/µl) at PN2/3 stage. Data represent mean ± s.e.m. (n ≥2 injection experiments). *** P&lt;0.001 High Daxx-EGFP versus other 3 groups; Fisher's exact test (on pooled embryos)."
NCBI gene IDs:
"EGFP: 
Tubulin: 
Daxx: 13163"
Figure legend: "(M) Left: IF of EGFP and endogenous H3 at pat-PCH in Daxxm-z+ PN5 zygotes expressing DAXX-EGFP or DAXXR244A-EGFP or nothing exogenously. Right: Fluorescence intensity profiles of H3 and EGFP along white lines in left panels at pat-PCH of zygotes. Scale bars 5 µm. Data information: All scale bars 10µm."
NCBI gene IDs:
"EGFP: 
Daxx: 13163
DAXX: 13163"
Figure legend: "(N) Boxplot displaying 3D quantification of H3 signal intensity at pat-PCH in Daxxm-z+ zygotes expressing exogenous DAXX-EGFP (n=14) or DAXXR244A-EGFP (n=13). In boxplots, the enter lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 2-3 times. ***P&lt;0.001 Daxx-EGFP versus other 2 groups; t-test."
NCBI gene IDs:
"EGFP: 
Daxx: 13163
DAXX: 13163"
Figure legend: "(A) RNA FISH of forward and reverse strand major satellite sequences in Daxxm+z+ and Daxxm-z+ PN5 zygotes. Scale bars, 10µm."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(B) Box plots indicating the foci number (left) and total intensity (right) of forward (top) and reverse (bottom) transcription as measured by RNA FISH on 3D paternal pronuclei in Daxxm+z+ and Daxxm-z+ PN5 zygotes. Lower hinge, central line and upper hinge in boxplots represent 25th, 50th (median) and 75th percentiles respectively. Upper/lower whiskers extend to the largest/smallest values no further than 1.5 * IQR from the upper/lower hinge, where IQR is inter-quartile range or distance between 25th and 75th percentiles. Datapoints are indicated by circles. Experiments were replicated 3 times. ***P&lt;0.001; t-test."
NCBI gene IDs:
"Daxx: 13163"
Figure legend: "(J) Boxplot showing FC (log2) in gene expression between Daxx deficient and control MII oocytes for five groups of genes classified according to FC (log2) in gene expression between Ring1 Rnf2 deficient and control GV oocytes. Boxplots were created and number of genes used for each boxplot is displayed below. Expression changes were estimated using 3 biological replicates for each condition. Statistical significance was estimated using two-sided t-tests."
NCBI gene IDs:
"Daxx: 13163
Ring1: 19763
Rnf2: 19821"
Figure legend: "(A)   Oil Red O staining   of mouse BAs differentiated  from normal or EP3-deficient brown pre-adipocytes. Scale bar: 50 μm. (B) Mean  lipid droplet area of differentiated BAs  from normal or EP3-deficient brown  pre-adipocytes. *P &lt; 0.05 vs WT (n = 6). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by Mann-Whitney U tests (B, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05"
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(D) Representative image of hematoxylin  and eosin  (H&amp;E) staining  for iBAT  from EP3F/F and  EP3F/FMyf5Cre mice. Scale bar: 50 μm. (E) Mean adipocyte area of BAs  in iBAT  from  EP3F/F and EP3F/FMyf5Cre mice  (n = 8). Data information: Data represent the mean ± standard error of mean (SEM). Data are pooled from two independent experiments with biological replicates Statistical significance was evaluated by Mann-Whitney U tests E, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"Cre: 2777477
Myf5: 17877
EP3: 19218"
Figure legend: "(K) Representative image of H&amp;E staining  of iBAT  from EP3F/F and EP3F/FMyf5Cre mice  fed HFD. Scale bar: 50 μm. (L) Mean adipocyte  area of BAs  in iBAT  from EP3F/F and EP3F/FMyf5Cre mice  fed HFD (n = 8). Data information: Data represent the mean ± standard error of mean (SEM). Data are pooled from two independent experiments with biological replicates L, Statistical significance was evaluated by Mann-Whitney U tests L, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"Cre: 2777477
Myf5: 17877
EP3: 19218"
Figure legend: "(A) N6-methyladenosine (m6A) modification levels of mRNAs in normal and EP3-deficient brown pre-adipocytes  with or without brown adipogenesis induction, detected by m6A RNA Methylation  Quantification Kit (Colorimetric) (n = 4-6). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by unpaired two-tailed student's t test (A *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(B)  qRT-PCR analysis of the relative mRNA levels of m6A  writers (METTL3, METTL4, METTL14  and WTAP) and erasers (FTO  and ALKBH5) in normal and EP3-deficient brown pre-adipocytes  with or without brown adipogenesis induction (n = 6-9). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by unpaired two-tailed student's t test B, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"ALKBH5: 268420
FTO: 26383
METTL14: 210529
METTL3: 56335
METTL4: 76781
EP3: 19218
WTAP: 60532"
Figure legend: "(C) Western blotting analysis of m6A  writers and erasers in normal and EP3-deficient brown pre-adipocytes with or without brown adipogenesis induction."
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(E) Western blotting analysis of WTAP  in iBAT  from EP3F/F and EP3F/FMyf5Cre mice  fed HFD  for 1 week."
NCBI gene IDs:
"Cre: 2777477
Myf5: 17877
EP3: 19218"
Figure legend: "(F)  Western blotting analysis of WTAP  in BAs differentiated  from EP3γ-expressed brown pre-adipocytes."
NCBI gene IDs:
"EP3γ: 19218"
Figure legend: "(G) Relative mRNA levels of WTAP  gene in BAs differentiated  from EP3γ-expressed brown pre-adipocytes  detected by RT-qPCR (n = 6-7). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates G, Statistical significance was evaluated by two-way ANOVA followed by Tukey's test for post-hoc comparisons (G, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"EP3γ: 19218
WTAP: 60532"
Figure legend: "(H) qRT-PCR analysis of the relative mRNA levels of EP3γ  gene in BAs  differentiated from EP3γ-expressed EP3-/- brown pre-adipocytes after transfection with WTAP  siRNA (n = 8). (I) qRT-PCR analysis of the relative mRNA levels of WTAP  gene in BAs  differentiated from EP3γ-expressed EP3-/- brown pre-adipocytes after transfection with WTAP  siRNA (n = 8). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by unpaired two-tailed student's t test H), two-way ANOVA followed by Tukey's test for post-hoc comparisons I, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"EP3γ: 19218
EP3: 19218
WTAP: 60532"
Figure legend: "(J) Representative picture of  oil red O-stained BAs differentiated  from EP3γ-expressed EP3-/- brown pre-adipocytes with WTAP  siRNA treatment. Scale bar: 50 μm."
NCBI gene IDs:
"EP3: 19218
EP3γ: 19218
WTAP: 60532"
Figure legend: "(K-Q) qRT-PCR analysis of the relative mRNA levels of brown marker genes in BAs  differentiated from EP3γ-expressed EP3-/- brown pre-adipocytes after transfection with WTAP  siRNA (n = 6-8). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by two-way ANOVA followed by Tukey's test for post-hoc comparisons K-Q). *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"EP3: 19218
EP3γ: 19218
WTAP: 60532"
Figure legend: "(A) Western blotting analysis of ubiquitination  of WTAP  in the control and EP3-deficient brown pre-adipocytes with or without sulprostone  treatment. Data information: Data are representative of two independent experiments with biological replicates"
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(B) Western blotting  analysis of ERK1/2 phosphorylation  in the control and EP3-deficient  brown pre-adipocytes. Data information: Data are representative of two independent experiments with biological replicates"
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(E) Analysis of PKA activity  in the control and EP3-deficient  brown pre-adipocytes (n = 4). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates"
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(F) Effect of PKA  inhibitor, H89, on ubiquitin-dependent degradation of WTAP  in EP3-/- brown pre-adipocytes. Data information: Data are representative of two independent experiments with biological replicates"
NCBI gene IDs:
"EP3: 19218"
Figure legend: "(G) Effect of ERK1/2 phosphorylation  site mutation (HA-WTAP-2A) on EP3-mediated ubiquitin-dependent degradation  of WTAP  in brown pre-adipocytes. Data information: Data are representative of two independent experiments with biological replicates"
NCBI gene IDs:
"HA: 
EP3: 19218
WTAP: 60532"
Figure legend: "(C)  Time course analysis of the relative mRNA levels of EP3  receptors in hESC-derived BAs  during BA differentiation  detected by RT-qPCR (n = 7-8). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates (C, Statistical significance was evaluated by Welch ANOVA test followed by Tamhane's T2 test for post-hoc comparisons (C) *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"EP3: 5733"
Figure legend: "(F) Effect of H89  on WTAP  mRNA expression  in hESC-derived BAs  treated with L798106  (n = 6-7). (G) Effect of H89  on ZNF410  mRNA expression  in hESC-derived BAs  treated with L798106  (n = 8). Data information: Data represent the mean ± standard error of mean (SEM). Data are representative of two independent experiments with biological replicates Statistical significance was evaluated by , two-way ANOVA followed by Tukey's test for multiple comparisons F, G). *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 and ns, not significant (P &gt; 0.05)."
NCBI gene IDs:
"WTAP: 9589
ZNF410: 57862"
Figure legend: "Ldb2 KO mice (red, n = 10-11) were compared to control (blue, n = 10-11) in Home cage-activity test (A). Voluntary locomotor activities in the home cages were monitored for a week. Data of dark (left) and light (right) phases are indicated."
NCBI gene IDs:
"Ldb2: 16826"
Figure legend: "Ldb2 KO mice (red, n = 10-11) were compared to control (blue, n = 10-11) in Open-field test (B)"
NCBI gene IDs:
"Ldb2: 16826"
Figure legend: "Ldb2 KO mice (red, n = 10-11) were compared to control (blue, n = 10-11) in Acoustic startle response (C)"
NCBI gene IDs:
"Ldb2: 16826"
Figure legend: "Ldb2 KO mice (red, n = 10-11) were compared to control (blue, n = 10-11) in Prepulse-inhibition test (D)"
NCBI gene IDs:
"Ldb2: 16826"
Figure legend: "Ldb2 KO mice (red, n = 10-11) were compared to control (blue, n = 10-11) in , Morris water-maze test (E) where data in hidden test (escape latency and moving speed, path length and no movement time) and probe test (path length) are shown"
NCBI gene IDs:
"Ldb2: 16826"