EMBO/SourceData huggingface dataset:
Figure legend: "Colonic organoids grown from Lgr5-EGFP-IRES-CreERT2 mice were treated with an AhR agonist (10 nM TCDD) or antagonist (10 µM CH223191) for 3 d. (B) Assessment of GFP+ stem cell percentages (n=3 replicates per treatment). Bars with different letters are significantly different (p&lt;0.05)."
NCBI gene IDs:
"EGFP: 
Cre: 2777477
ER: 2099
Lgr5: 14160"
Figure legend: "(D) Flow cytometry analysis of GFP+Tomato+ and Tomato+ cells in the large intestine of AhR wild type (Lgr5-GFPCreERT2 X Tomatof/f, GT) and AhR knock out (AhRf/f X Lgr5-GFPCreERT2 X Tomatof/f, HGT) mice. Top left panel shows representative cells from WT negative control animal (no GFP or Tomato expression). Top right panel shows Tomato only control (CDX2P-CreERT2 X Tomatof/f). Middle panels represent DMSO or TCDD treatment in AhR WT mice, respectively. Mice were gavaged every other day, 4 times total, with vehicle or TCDD (25 µg/kg bw) and terminated 1 d later. Bottom left panel is from a representative AhR WT mouse and bottom right panel is from a representative AhR KO mouse."
NCBI gene IDs:
"GFP: 
Tomato: 
AhR: 11622
CDX2: 1045
Cre: 2777477
ER: 2099
Lgr5: 14160"
Figure legend: "(E) Induced Cyp1a1 mRNA expression in stem cells after oral gavage with TCDD (n=4)."
NCBI gene IDs:
"Cyp1a1: 13076"
Figure legend: "Flow cytometry analysis of GFP+Tomato+ and Tomato+ cells in the large intestine of AhR wild type and AhR knock out mice. (G) Quantitative analysis of the percentage of Lgr5+ stem cells in (D), n=7-10 mice per group."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(A) Representative flow cytometry gating and fluorescence microscopy images of sorted GFPhigh stem cells and GFPlow progenitor cells from Lgr5-EGFP-IRES-CreERT2 mice."
NCBI gene IDs:
"EGFP: 
Cre: 2777477
ER: 2099
Lgr5: 14160"
Figure legend: "(B) Representative brightfield images of organoids generated from GFPhigh stem cells isolated from tamoxifen treated AhR WT mice (Lgr5-GFPCreERT2 X CDX2P-CreERT2 [GC]) and KO mice (AhRf/f X Lgr5-GFPCreERT2 X CDX2P-CreERT2 [HGC]), treated with DMSO (control) or TCDD for 5 d. Data are from ≥4 mice, scale bar 200 µm."
NCBI gene IDs:
"GFP: 
AhR: 11622
CDX2: 1045
Cre: 2777477
ER: 2099
Lgr5: 14160"
Figure legend: "(C, D) The organoid forming efficiency (C) and diameter (D) from sorted GFPhigh stem cells in AhR WT or KO mice treated for 5 d with DMSO or TCDD (10 nM). n=4-5 mice per group in (C). Each dot represents an individual organoid and grey line indicates mean in (D). Bars with different letters are significantly different (p&lt;0.05)."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(H, I) (H) Lgr5 expression in organoids and (I) sorted stem cells in the absence or presence of TCDD treatment (n≥3 independent samples per group)."
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "(C) Heatmap comparison of the top 25 upstream regulators between AhR KO (KO/WT) and TCDD (TCDD/vehicle in WT mice) treated cells."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(A) FoxM1, Plk1, and Cdc25B mRNA expression in stem and progenitor cells with and without AhR, n=4 mice per group."
NCBI gene IDs:
"AhR: 11622
Cdc25B: 12531
FoxM1: 14235
Plk1: 18817"
Figure legend: "(B) FoxM1, Plk1, and Cdc25B mRNA expression in WT stem and progenitor cells with and without TCDD, n=3-4 mice per group."
NCBI gene IDs:
"Cdc25B: 12531
FoxM1: 14235
Plk1: 18817"
Figure legend: "Chromatin immunoprecipitation (ChIP) analysis of AhR and FoxM1 promoter interaction using WT colonic organoids treated with DMSO or TCDD, n=3 or 4 per group. A DNA fragment amplified in a gene desert on mouse chromosome 6 was used as a negative control."
NCBI gene IDs:
"FoxM1: 14235"
Figure legend: "Chromatin immunoprecipitation (ChIP) analysis of AhR and FoxM1 promoter interaction using WT and KO crypts (E) treated with DMSO or TCDD, n=3 or 4 per group."
NCBI gene IDs:
"FoxM1: 14235"
Figure legend: "(G Expression of FoxM1 and PLK1 at mRNA level, , n&gt;=3 independent organoids per treatment."
NCBI gene IDs:
"FoxM1: 14235
PLK1: 18817"
Figure legend: "(L) Organoid expression of Lgr5 in response to FDI-6. Lgr5 expression level was dramatically suppressed by FDI-6 in a dose-dependent manner, n&gt;=3 per treatment."
NCBI gene IDs:
"Lgr5: 14160"
Figure legend: "(A and B) Proliferating Lgr5+ stem cells marked by EdU in the distal colon from AhR WT (GT) and KO (HGT) mice. Arrowhead and star symbols denote proliferating stem cells (GFP+EdU+) and static stem cells (GFP+EdU-), respectively, during a 2 h EdU pulse, n=3 per treatment."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(C) Total number of EdU+ cells per crypt in the distal colon of AhR WT and KO (HGT) mice, n=3 mice per treatment. Dashed line indicates median and dotted lines indicate 25th/75th quartiles."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(D&amp;E) Crypt cell proliferative zone analysis in AhR WT and KO (HGT) mice, presented as the distance from the crypt base to the highest EdU+ cell divided by total crypt length. Red lines between crosses show the measurement of distance. Each symbol represents one crypt, n=3 mice per treatment, scale bar 20 µm. Grey line represents mean."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(F&amp; AhR KO promoted cell proliferation 5 d post DSS treatment. AhR WT (AhRf/f [H], n=4) and AhR KO (AhRf/f X Villin-Cre [HV], n=4) mice were used."
NCBI gene IDs:
"AhR: 11622
Cre: 2777477
Villin: 22349"
Figure legend: "G) AhR KO promoted cell proliferation 5 d post DSS treatment. Data represent mean ± SEM, quantified as the ratio of the EdU+ area relative to the DAPI+ area using KeyenceTM software."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(H) Increased cell proliferation (EdU+ cells) observed in AhR KO vs WT organoids derived from HGC and GC mice, respectively (n=6 from 3 mice per group)."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(I) Effect of AhR KO on cell cycle S and G2-M phases (n=3 mice per group)."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(B) Representative colon images from AhR WT and AhR KO mice following AOM/DSS treatment. Scale bar, 9 mm."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(C) H&amp;E staining of representative colon tumor sections from AhR WT and KO mice, scale bar 200 μm."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(E, F) (E) Adenoma and (F) adenocarcinoma multiplicity in AhR WT and KO mice."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(G) Tumor volume in AhR KO and WT mice. Grey line indicates mean."
NCBI gene IDs:
"AhR: 11622"
Figure legend: "(H) FoxM1 mRNA expression in normal (uninvolved) crypts adjacent to colonic tumors. Images were quantified and expressed as FoxM1 positive area per crypt (n=5 mice per group). Scale 200 µm. Dashed line indicates median and dotted lines indicate 25th/75th quartiles."
NCBI gene IDs:
"FoxM1: 14235"
Figure legend: "(I) FoxM1 mRNA expression in colon tumors. FoxM1 mRNA ISH area was averaged per tumor on each slide (n=5 mice per group). Scale 200 µm. Dashed line indicates median and dotted lines indicate 25th/75th quartiles."
NCBI gene IDs:
"FoxM1: 14235"
Figure legend: "(A-B) mRNA expression of human colonic stem cell markers LGR5 and OLFM4, n=5 or 6 independent human samples. Paired student t-test."
NCBI gene IDs:
"LGR5: 8549
OLFM4: 10562"
Figure legend: "(C) Representative images of LGR5 ISH in human organoids treated with DMSO or TCDD. Scale bar 50 µm."
NCBI gene IDs:
"LGR5: 8549"
Figure legend: "(I) Determination of luciferase reporter activity of human FoxM1 in SW48 and Caco2 cell lines treated with DMSO or TCDD. n=3-4 per group."
NCBI gene IDs:
"FoxM1: 2305"
Figure legend: "(J) FOXM1 and CYP1A1 mRNA expression in different AhR WT and CRISPR KO clones treated with TCDD for 1 d. C56 and C61 designate different human subjects."
NCBI gene IDs:
"AhR: 196
CYP1A1: 1543
FOXM1: 2305"
Figure legend: "(K) Validation of CRISPR KO clones was assessed by PCR amplification using primers flanking the targeted AhR exon. Subsequent sequencing revealed indels at the expected locations."
NCBI gene IDs:
"AhR: 196"
Figure legend: "(L) The association of FoxM1 and AhR mRNA expression in human normal colon biopsies (n=304). AhR low (n=152) is defined as below its median expression; AhR high (n=152) is defined as above its median expression. Dashed line indicates median and dotted lines indicate 25th/75th quartiles."
NCBI gene IDs:
"AhR: 196
FoxM1: 2305"
Figure legend: "(M) Correlation between FoxM1 and Cyp1b1 mRNA expression in human normal colon biopsies (n=303) analyzed by Spearman rank correlation."
NCBI gene IDs:
"Cyp1b1: 1545
FoxM1: 2305"
Figure legend: "(a) Ultrastructural evidence of autophagy induced by depletion of p53 with a specific siRNA or pharmacological inhibition of p53 with PFT-α in HCT116 cells."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(b) Autophagy induced by PFT-α, p53 knockdown, or p53 knockout in HCT116 cells. The number of autophagosomes and autolysosomes was determined for at least 50 cells in 3 independent experiments (mean ± s.e.m.; *P 0.05). Culture in nutrient-free (NF) conditions was used as a positive control."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(c) Effect of p53 on the maturation of LC3. Immunoblots are shown for wild-type (WT) or p53−/− HCT116 (n = 3)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(d) GFP-LC3 puncta induced by PFT-α, p53 knockdown or knockout. WT and p53−/− HCT116 cells were transfected with control or p53-specific siRNAs, re-transfected with a GFP-LC3 plasmid, cultured in complete medium for 24 h and kept for 6 h in the presence or absence of 30 μM PFT-α. The percentage of cells showing accumulation of GFP-LC3 in puncta (GFP-LC3vac) is reported (mean ± s.d., n = 3; *P 0.05)."
NCBI gene IDs:
"LC3: 440738///81631///84557
p53: 7157"
Figure legend: "(e) GFP-LC3 puncta in p53−/− MEF, compared with WT MEF transfected with GFP-LC3 (mean ± s.d., n = 3; *P 0.05)."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(f) GFP-LC3 dots in tissues from p53+/+ or p53−/− mice expressing a GFP-LC3 transgene (mean ± s.d.; *P 0.05), without or after 24 h of starvation. The number of GFP-LC3 dots per area was determined for a minimum of 4 fields (at ×400 magnification for 4 slides per animal, 3 animals per condition)."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(g) TEM pictures of p53−/− and p53+/− mouse livers. p53−/− hepatocytes carry approximately 3 autophagosomes, p53+/− controls approximately 1 per cell."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(h) Modulation of DsRed::LGG-1 levels by the p53 orthologue CEP-1 in C. elegans. Representative pictures are shown for WT animals and for nematodes carrying a cep-1 deletion allele (gk138). The count of puncta per cell was 6.9 ± 3.3 for WT, 52.2 ± 9.4 for cep-1 (gk138) embryos (mean ± s.e.m. for 20 individuals, 5 cells per embryo). The quantification of DsRed::LGG-1 puncta is shown for the indicated genetic backgrounds and conditions (dots represent pixel intensity for individual embryos)."
NCBI gene IDs:
"cep-1: 172616
CEP-1: 172616"
Figure legend: "(a) PFT-α or p53 siRNA-induced GFP-LC3 puncta in WT HCT116 cells transfected with siRNAs to deplete the essential autophagy gene products Atg5, Atg10, Atg12, Beclin 1 or hVps34. Culture in nutrient-free (NF) conditions was used as a control of autophagy induction."
NCBI gene IDs:
"Atg10: 83734
Atg12: 9140
Atg5: 9474
Beclin 1: 8678
hVps34: 5289
p53: 7157"
Figure legend: "(b) PFT-α or p53 siRNA-mediated GFP-LC3 puncta in MEFs transfected with siRNAs specific for mouse Atg5 or Beclin 1."
NCBI gene IDs:
"Atg5: 11793
Beclin 1: 56208
p53: 22059"
Figure legend: "(c) Requirement of Beclin 1 expression for autophagy induction by p53 depletion in MCF7 cells. MCF7 cells stably transfected with a tetracycline-repressible Beclin 1 construct were maintained in conditions that prevent or allow Beclin 1 expression, transfected with GFP-LC3 and subjected to p53 knockdown before quantification of GFP-LC3 puncta. Data in a-c are mean ± s.d. of 3 independent experiments."
NCBI gene IDs:
"Beclin 1: 8678
p53: 7157"
Figure legend: "(a) Immunoblot detection of the phosphorylation status of AMPKα, ACCα, TSC2 and p70S6K in HCT116 cells. Hypophosphorylation of p70S6K and hyperphosphorylation of AMPKα, ACCα and TSC2 was detected in p53−/− HCT116, compared with WT HCT116 cells after culture in complete medium (n = 5)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(b) Quantification of GFP-LC3vac cells in WT and p53−/− HCT116 cells transfected with siRNAs specific for the catalytic α1 and α2 subunits of AMPK (mean ± s.d. of 3 independent experiments; *P 0.05). The inset in b demonstrates the efficiency of siRNA-mediated downregulation of AMPKα1 and AMPKα2 , as assessed by immunoblot analysis (n = 3). Note that the antibody recognizes both AMPKα1 and AMPKα2."
NCBI gene IDs:
"AMPKα1: 5562
AMPKα2: 5563
p53: 7157"
Figure legend: "(c) Immunoblot detection of ACCα phosphorylation in WT and p53−/− HCT116 cells subjected to knockdown of AMPKα1 and AMPKα2 (n = 5); Co, control."
NCBI gene IDs:
"AMPKα1: 5562
AMPKα2: 5563
p53: 7157"
Figure legend: "(d) Quantification of GFP-LC3vac cells (means ± s.d., n = 3 separate experiments) in WT HCT116 and p53−/− HCT116 treated with PFT-α and/or rapamycin."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(a) Cytosolic ATP levels in WT and p53−/− HCT116 cells in the absence of glucose. Measurements were performed on luciferase-expressing HCT116, after perfusion of the cells with medium first containing then missing glucose."
NCBI gene IDs:
"luciferase: 
p53: 7157"
Figure legend: "(b) Effect of methylpyruvate (MetPyr) on ATP levels in WT p53−/− HCT116 cells depleted of glucose. ATP levels were measured as in a, in the presence of glucose and 15 min after its withdrawal or replacement by MetPyr."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(c) Effect of autophagy on the ATP levels of WT and p53−/− HCT116 cells depleted of glucose. Cells were transfected with siRNAs specific for Beclin 1 and AMPKα, or they were treated with rapamycin for 6 h, followed by measurement of ATP levels as in a, before or after glucose withdrawal (means ± s.d. of triplicates, 3 independent experiments)."
NCBI gene IDs:
"Beclin 1: 8678
AMPKα: 5563///5562
p53: 7157"
Figure legend: "(d) Metabolic stress-induced cell death is attenuated in the absence of p53. HCT116 cells were transfected with control, Atg5-, Atg10-, or AMPKα-specific siRNAs and were subjected 48 h later to metabolic stress (cultured for 48 h in nutrient-free, hypoxic conditions) and stained with DiOC6(3) and PI. The black and white portions of the columns refer to the DiOC6(3)low PI− (dying) and DiOC6(3)low PI+(dead) population, respectively."
NCBI gene IDs:
"Atg10: 83734
Atg5: 9474
AMPKα: 5563///5562
p53: 7157"
Figure legend: "(e) Metabolic stress-induced decrease of clonogenic survival was attenuated in the absence of p53. HCT116 cells subjected to metabolic stress as in d were monitored for clonogenic survival."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(f) Expression of Beclin 1 in MCF7 cells restores the survival advantage conferred by p53 depletion. MCF7 cells that carry a tetracycline-repressible Beclin 1 expression construct were cultured to avoid Beclin 1 expression or to induce it, transfected with a control siRNA or a p53-specific siRNA, subjected to metabolic stress, and finally stained with DiOC6(3)/PI."
NCBI gene IDs:
"Beclin 1: 8678
p53: 7157"
Figure legend: "(g) Effect of p53 and autophagy on the survival of metabolically stressed MEFs. WT MEFs were transfected with the indicated siRNAs and then subjected to metabolic stress before performing clonogenic assays. Results in d-g are mean ± s.d. of 3 separate experiments (*P 0.05); Co, control."
NCBI gene IDs:
"p53: 22059"
Figure legend: "(c-e) Effect of p53 mutants on autophagy in p53−/− HCT116 cells.Representative micrographs of cells transfected with plasmids expressing WT, nuclear and cytoplasmic p53 are shown in d"
NCBI gene IDs:
"p53: 7157"
Figure legend: "(c-e) Effect of p53 mutants on autophagy in p53−/− HCT116 cells. Quantification of GFP-LC3 puncta of p53−/− HCT116 cells transiently transfected with p53 mutants is shown in e (mean ± s.d., n = 3 experiments, *P 0.05)"
NCBI gene IDs:
"p53: 7157"
Figure legend: "(f) Detection of the phosphorylation status of AMPKα, ACCα, TSC2 and p70S6K in p53−/− HCT116 cells transiently expressing WT or mutant p53 (n = 5)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(d) eIF2α phosphorylation in WT or p53−/− HCT116 cells treated with PFT-α (n = 5)."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(e, f) WT HCT116 cells (e, f) or p53−/− HCT116 cells (f) were transfected with GFP-LC3, plus a control siRNA or the indicated combination of IRE1α- and/or p53-specific siRNA, and treated with PFT-α, thapsigargin, tunicamycin or brefeldin A."
NCBI gene IDs:
"IRE1α: 2081
p53: 7157"
Figure legend: "(g) GFP-LC3-transfected WT or ire1α−/− MEF were treated with PFT-α. Columns in e-g show means ± s.d. of 3 independent experiments (*P 0.05)."
NCBI gene IDs:
"ire1α: 78943"
Figure legend: "(c) WT HCT116 cells transfected with control or HDM2-targeted siRNAs were cultured in the presence or absence of MG132 for 3 h, followed by the treatment with autophagy inducers for 6 h (Tp, thapsigargin; other abbreviations as in b). The abundance of p53 and HDM2 was determined by immunoblotting analysis and the percentage of cells showing accumulation of GFP-LC3 in vacuoles (GFP-LC3vac) was determined."
NCBI gene IDs:
"HDM2: 4193"
Figure legend: "(e) Effect of MG132 and HDM2 siRNA on autophagy of WT and p53−/− HCT116 cells."
NCBI gene IDs:
"HDM2: 4193
p53: 7157"
Figure legend: "(f) Failure of HDM2 inhibitors to prevent autophagy induced by the absence of p53. Nutlin-3 and RITA were added to p53−/− HCT116 cells, followed by quantification of GFP-LC3 puncta."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(g) Effect of WT and ubiquitination-resistant p53 on autophagy. p53−/− HCT116 cells were transfected with GFP-LC3 alone or in combination with different concentrations of pcDNA3.1, WT p53 or p53 lacking the ubiquitination site at amino acids 13-19 (p53ΔI), then treated with rapamycin, tunicamycin or thapsigargin. Thereafter, p53 expression and GFP-LC3 puncta were quantified."
NCBI gene IDs:
"p53: 7157"
Figure legend: "(h) Depletion of Atg5 or Beclin 1 does not prevent p53 degradation triggered by rapamycin or ER stressors. In WT HCT116 cells transfected with siRNA targeting Atg5 or Beclin 1, p53 levels were determined by immunoblot analysis after 6 h of treatment with rapamycin, tunicamycin or thapsigargin."
NCBI gene IDs:
"Atg5: 9474
Beclin 1: 8678"
Figure legend: "C TIMM50S levels in primary and immortalized skin fibroblast from control (C1-4) and mutant TIMM50 (Pt). SDHA and ETHE1 are shown as loading controls."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "D qPCR quantification of TIMM50 transcript levels in control and TIMM50 mutant fibroblasts. Data are shown as mean ± SD, n = 4."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "A Western-blot of whole cell extracts for different components of the protein import machinery in control and TIMM50 mutant fibroblasts grown in either glucose or galactose containing medium."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "B Western-blot of whole cell extracts for different components of the protein import machinery in control and TIMM50 mutant fibroblasts, transduced with the empty vector (EV) or with wild-type TIMM50 grown in either glucose or galactose containing medium."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "D TMRM live staining of control and TIMM50 mutant fibroblasts, transduced with the empty vector (EV) or with wild-type TIMM50 grown in either glucose or galactose containing medium. Scale bar corresponds to 10 μm."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "A In organello import of the precursors of TFAM and AAC1 into isolated mitochondria from control and TIMM50 mutant fibroblasts in the absence or presence of the uncoupler FCCP. The radiolabeled precursors were incubated with isolated mitochondria for the indicated times followed by trypsin treatment only in the case of AAC1. Coomassie Blue (CB) staining was used as loading control and the radiolabeled protein (TNT) as reference for quantification. The position of the precursor (empty arrowheads) and the mature protein (filled arrowhead) are also shown. Western blot for TIMM50 is shown as cell line control. B Quantification of TFAM and AAC1 import from control and TIMM50 mutant fibroblasts expressed as fraction of the input radiolabeled precursor converted into mature protein and normalized to Coomassie Blue signal. Data are shown as mean ± SD, n = 3 biological replicates; ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗*∗p &lt; 0.001 grouping both controls together. "
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "C In organello import of the precursors of TFAM and AAC1 into isolated mitochondria from control and TIMM50 mutant fibroblasts transduced with the empty vector (EV) or with wild-type TIMM50 in the absence or presence of the uncoupler FCCP The position of the precursor (empty arrowheads) and the mature protein (filled arrowhead) are also shown. D Quantification of TFAM and AAC1 import from control and TIMM50 mutant fibroblasts transduced with the empty vector (EV) or with wild-type TIMM50 expressed as fraction of the input radiolabeled precursor converted into mature protein and normalized to Coomassie Blue signal. Data are shown as mean ± SD, n = 3 biological replicates; ∗p &lt; 0.05. "
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "Western blot for representatives of all five respiratory complexes in control and TIMM50 mutant fibroblasts grown in glucose or galactose. Two biological replicates are shown for each cell line and condition. Significant changes in protein levels in TIMM50 mutant fibroblasts compared to controls have been boxed in red."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "B Western blot for representatives of all five respiratory complexes in control and TIMM50 mutant fibroblasts, transduced with the empty vector (EV) or with wild-type TIMM50 grown in glucose or galactose. Two biological replicates were obtained for each cell line and condition. Significant changes in protein levels in TIMM50 mutant fibroblasts complemented with TIMM50 compared to EV have been boxed in red."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "Oxygen consumption measurements in control and TIMM50 mutant fibroblasts grown in glucose or galactose. Values of basal and maximal respiration along with ATP-production dependent, proton leak respiration and spare capacity are shown. Data are shown as mean ± SD, n = 4 biological replicates; ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗*∗p &lt; 0.001 grouping both controls together."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "D Oxygen consumption measurements in control and TIMM50 mutant fibroblasts, transduced with the empty vector (EV) or with wild-type TIMM50 grown in glucose or galactose. Values of basal and maximal respiration along with ATP-production dependent, proton leak respiration and spare capacity are shown. Data are shown as mean ± SD, n = 4 biological replicates; ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗*∗p &lt; 0.001."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "A ROS production as measured by intracellular oxidation of the cell-permeant probe DCF-DA in control and TIMM50 mutant fibroblasts grown in glucose or galactose. Measurements were made in untreated cells and after treatment with 250μM hydrogen peroxide for 30 min. Data are shown as mean ± SD, n = 3 biological replicates; ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗*∗p &lt; 0.001 grouping both controls together."
NCBI gene IDs:
"TIMM50: 92609"
Figure legend: "B Steady state levels of ROS related proteins SOD2 and ACO2 in control and TIMM50 mutant fibroblasts growing in either glucose or galactose were assessed by Western blot using GAPDH as internal control. C Quantification of the steady state levels of SOD2 and ACO2 in control and TIMM50 mutant fibroblasts growing in either glucose or galactose. Data are shown as mean ± SD, n = 4 biological replicates; ∗p &lt; 0.05, ∗∗p &lt; 0.01 grouping both controls together."
NCBI gene IDs:
"TIMM50: 92609"