EMBO/SourceData huggingface dataset:
Figure legend: "B. Traces showing average changes (mean±s.e.m.) in  intracellular Ca2+ (Fura2 ratio) over  time  in response to perfusion of different [Ca2+]o as indicated in the upper bar with constructs as indicated expressed in HEKO1.  STIM1  (black, n=145), STIM1A  (red, n=157) and STIM1A_D503A (n=185). C. Relative fluorescence intensities of mCherry-tagged constructs measured in (B). D. Quantification of changes in resting ratio, TG  peak (∆ ratio) and SOCE parameters measured in B. E. Data information: *P&lt;0.05, **P&lt;0.01 ***P&lt;0.001 Kruskal-Wallis Anova with Dunn's multiple comparisons test. Data points (total n) were obtained from three biological replicates with each three technical replicates (each multiple cells) and shown as mean±s.e.m for traces and as scatter plots with the underlying boxes showing the means for individual parameters."
NCBI gene IDs:
"O1: 84876
STIM1: 6786
STIM1A: 6786"
Figure legend: "E. Average traces showing  whole-cell  current density  (CD) over  time extracted at -80 mV in HEKO1  cells transfected with  STIM1 (black),  STIM1A (red) or  STIM1A_D503A (blue). F. Average maximum  CDs recorded from cells measured in E (n within bars). G Data information: *P&lt;0.05, **P&lt;0.01 ***P&lt;0.001 Kruskal-Wallis Anova with Dunn's multiple comparisons test. Data points (total n) were obtained from three biological replicates with each three technical replicates (each multiple cells) and shown as mean±s.e.m for traces and as scatter plots with the underlying boxes showing the means for individual parameters."
NCBI gene IDs:
"O1: 84876
STIM1: 6786
STIM1A: 6786"
Figure legend: "F. Traces showing average changes (mean±s.e.m.) in  intracellular Ca2+ (Fura2 ratio) over  time in response to perfusion of different [Ca2+]o as indicated in the upper bar with constructs as indicated expressed in HEKS1/S2-/-. G. Quantification of the relative reduction of ratio/time  or ratio for cells (135 H Data information: , ***P&lt;0.001, Kruskal-Wallis Anova with Dunn's multiple comparisons test Data points (total n) were obtained from three biological replicates with each three technical replicates (with multiple cells) and shown as mean±s.e.m for traces (F) and as scatter plots with the underlying boxes showing the means for individual parameters (G)."
NCBI gene IDs:
"S1: 6786
S2: 57620"
Figure legend: "H,I. Average traces (mean±s.e.m.) showing  whole- cell current density (CD) over  time extracted at -80 mV in in HEKS1/S2-/- cells co-transfected either with STIM1  (black) or STIM1A (red) and ORAI1 (H) or with ORAI1  R77E (I) and recorded using extracellular solution containing 2 mM  Ca2+. J. Average maximum  CDs recorded from cells measured in [H,I] (n within bars). Data information: I , *P&lt;0.05, **P&lt;0.01, unpaired T test with Welch's correction. For patch-clamp experiments, number in bars indicate measured cells, which were from three independent transfections."
NCBI gene IDs:
"ORAI1: 84876
S1: 6786
STIM1: 6786
STIM1A: 6786
S2: 57620"
Figure legend: "B. Hits from (A) tested for interaction  with commercially available constructs quantified with  bimolecular fluorescence complementation (BiFC) via  flow cytometry. HEKS1/S2-/- were transfected with  STIM1-YFPC (black) or STIM1A-YFPC (red) in combination with POI-YFPN and screened for YFP+ cells via  FACS, results (mean ± SD) from three transfections with each 10.000 sorted cells."
NCBI gene IDs:
"YFP: 
S1: 6786
STIM1: 6786
STIM1A: 6786
S2: 57620"
Figure legend: "D. Ratio  nuclear NFAT-GFP  vs. cytosolic GFP  intensity normalized to t=0 (mean±s.e.m.) after transfection of SH-SY5Y S1-/- cells with STIM  (n=42), STIM1A (n=82), STIM1A_D503A (n=49) or vector only (ØSTIM, n=15) IRES mCherry and induction of SOCE after stimulation with 1 µM TG. Data (total n as indicated) from at least three independent transfections."
NCBI gene IDs:
"mCherry: 
STIM: 6786
S1: 6786
STIM1A: 6786"
Figure legend: "A. Time course  of translocation (Nuclear NFAT-GFP signal/cytosolic signal normalized to TG addition at t=0, mean±s.e.m, for each time point) after transfection of indicated constructs and preincubation with 1 µM PF-04957325 (analyzed cells: STIM1 + PF n = 75, STIM1A + PF  n = 50, ØSTIM + PF n = 9) Data (total n as indicated) from at least three independent transfections. * p&lt; 0.05, **p&lt;0.01, ***p&lt;0.001, Kruskal-Wallis Anova with Dunn's multiple comparisons test. B. PF-04957325 induced difference of the  nuclear NFAT-GFP signal vs. cytosolic signal from (A) to mean of control without the blocker (see Fig. 6D). * p&lt; 0.05, **p&lt;0.01, ***p&lt;0.001,Mann-Whitney test of differences.. C Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, 2way Anova Data (total number of cells, n) were obtained from at least three independent transfections with several technical replicates each."
NCBI gene IDs:
"STIM: 6786
STIM1: 6786
STIM1A: 6786"
Figure legend: "C. Endpoint value with addition of PF or indicated cAMP analoga loaded for 30 min as AM esters before the experiment, shown as mean±s.e.m , ØSTIM + 6Bnz-cAMP/8-pCPT-cAMP n = 15/14 cells; STIM1  +  6Bnz-cAMP/8-pCPT-cAMP: n = 87/66, STIM1A + 6Bnz-cAMP : n = 78/67; from three independent transfections. Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, , Kruskal-Wallis Anova with Dunn's multiple comparison test Data (total number of cells, n) were obtained from at least three independent transfections with several technical replicates each."
NCBI gene IDs:
"STIM: 6786
STIM1: 6786
STIM1A: 6786"
Figure legend: "G. Normalized fluorescence signal change (mean±s.e.m) of  R-FlincA, SH-SY5Y S1-/- cells expressing STIM1 (n=20) or  STIM1A  (n=22) IRES  GFP variants and the indicator were stimulated by TG (1 μM). To avoid photobleaching, values were only recorded after 5 and 30 min, respectively The mutant R-Flinc (n=15) was measured in SH-SY5Y  wt cells after TG stimulation. Data information: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, Kruskal-Wallis Anova with Dunn's multiple comparison test Data (total number of cells, n) were obtained from at least three independent transfections with several technical replicates each."
NCBI gene IDs:
"GFP: 
S1: 6786
STIM1: 6786
STIM1A: 6786"
Figure legend: "Rfa1-TAP purification was performed using Rfa1-TAP rtt105∆ strains expressing WT and indicated Rtt105 mutant forms. pRS313 serves as vector control (Vc)."
NCBI gene IDs:
"rtt105: 856841
Rtt105: 856841"
Figure legend: "Snapshots of Rfa1 ChIP-Seq peak at ARS305 from WT and rtt105∆ cells released into HU-medium. The average Rfa1 ChIP-Seq read density from cells released into HU-medium around ACS sites. ACS: ARS consensus sequence."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "The nuclear localization of Rfa1 proteins is altered in rtt105∆ mutant cells. Rfa1 tagged with GFP at its C terminus was used to analyze the localization of Rfa1-GFP fusion protein (Green) and the nuclear envelop is visualized with the Nup49-mCherry fusion protein (Red) using fluorescence microscopy. DIC: differential interference contrast. Scale bar: 5μm."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "NLS-RFA1-GFP rescues the nuclear localization of Rfa1 in rtt105∆ mutant cells. The wild type RFA1 gene was fused with an SV40 large T Antigen nuclear localization sequence (NLS) at its 5'-end and a GFP gene at its 3' end to obtain a construct, driven by the RFA1 promoter to express NLS-RFA1-GFP fusion protein. The engineered construct was then transformed into WT or rtt105∆ mutant yeast cells to replace its endogenous RFA1 expression. The resulting yeast cells were then visualized under microscope, and GFP signals enriched in the nuclei were scored. RFA1-GFP lacking the NLS sequence was transformed into rtt105∆ rfa1∆ mutant cells as a control. DAPI staining indicates nuclear DNA."
NCBI gene IDs:
"GFP: 
RFA1: 851266
rfa1: 851266
rtt105: 856841
large T Antigen: 29031019"
Figure legend: "An increase in nuclear localization of Rfa1 could not rescue the RPA binding defects at the replication regions in rtt105∆ cells. The percentage of Rfa2 ChIP-DNA over the total input DNA was calculated. The mean and standard error (SE) of three biological replicates were shown. Statistical significance was evaluated based on Student's t-tests (*: 0.01≤P value&lt;0.05)."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "Rad52-GFP foci were counted in WT and rtt105∆ cells, the percentage of cells with Rad52-foci is reported. Error bars indicate one standard deviation from three independent experiments with at least 150 cells counted in each replicate."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "Rad53 phosphorylation was analyzed in WT and rtt105∆ cells by immunoblotting of protein extracts with anti-Rad53 antibodies. Ponceau S (Pon S) staining was applied as a loading control."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "Deletion of RTT105 leads to an increased rate of gross chromosomal rearrangements (GCRs). Wild-type (WT) and rtt105∆ mutant strains integrated with the yWSS439-5ori∆ yeast artificial chromosomes (YACs). GCRs rates in the evaluation of telomere marker loss were assayed"
NCBI gene IDs:
"YACs: 
yeast artificial chromosomes: 
RTT105: 856841
rtt105: 856841"
Figure legend: "Ten-fold serial dilutions WT or rtt105∆ cells in two different backgrounds were assayed on normal growth media (YPD) and on media containing the indicated DNA damaging agents, methyl-methane sulfonate (MMS), camptothecin (CPT), bleomycin (Bleo), and hydroxyurea (HU)."
NCBI gene IDs:
"rtt105: 856841"
Figure legend: "C. Effect of Nox4 on the unfolded protein response. Nox4 was depleted in H9c2 cells by shRNA-mediated knockdown (Ad.shNox4) or cells were treated with a control adenovirus (Ad.Ctl). In cells with Nox4 knockdown, tunicamycin treatment resulted in lower increases in protein levels of the ER chaperones Grp94, Grp78 and calreticulin than in conrol cells. Nuclear protein levels of ATF4 were substantially lower in Nox4-depleted cells than control cells but the levels of cleaved ATF6 (ATF6c) were similar. Histone was used as a loading control. The relative mRNA levels of Xbp1-s (a readout of IRE1 signaling) were unaltered after Nox4 knockdown. Mean data are shown in Appendix Fig S1E. Similar results were obtained with an independent siRNA approach (Appendix Fig S2A).D. Effect of adenoviral-mediated overexpression of Nox4 (Ad.Nox4) or a control β-galactosidase protein (Ad.β-Gal) on tunicamycin responses of H9c2 cells. Nox4 enhanced the increase in cellular ER chaperones and nuclear ATF4 levels but did not affect tunicamycin-induced changes in nuclear ATF6c levels and caused minor reduction in Xbp1s mRNA levels. Mean data are shown in Appendix Fig S2B."
NCBI gene IDs:
"β-Gal: 
β-galactosidase: 
Nox4: 85431
Xbp1: 289754"
Figure legend: "E,F. Effect of Nox4 knockdown or overexpression, respectively, on the tunicamycin-induced changes in mRNA levels of ATF4 target genes. n=4/group. Psat1, phosphoserine aminotransferase; Phgdh, 3-phosphoglycerate dehydrogenase; Asns, asparagine synthetase; Slc6a9, glycine transporter 1."
NCBI gene IDs:
"Asns: 25612
ATF4: 79255
Nox4: 85431
Phgdh: 58835
Psat1: 293820
Slc6a9: 116509"
Figure legend: "G. Effect of ATF4 silencing with two different siRNAs on Nox4 protein levels in tunicamycin-treated H9c2 cells. Scrambled siRNAs were used as a control (Ctl). Representative immunoblots shown to the top (captions at bottom of bar graphs refer also to the immunoblots); tubulin was used as a loading control. n=4/group. *, significant compared to baseline; #, significant comparing siATF4 versus corresponding siCtl."
NCBI gene IDs:
"ATF4: 79255"
Figure legend: "H. Effect of ATF4 overexpression on Nox4 mRNA and protein levels. n=3/group."
NCBI gene IDs:
"ATF4: 79255"
Figure legend: "A. The knockdown of endogenous Nox4 resulted in a substantial inhibition of tunicamycin-induced eIF2a phosphorylation in H9c2 cells, with no change in phospho-Thr980-PERK (PERK-P) levels. GADD34 levels were significantly decreased after Nox4 knockdown while there was no change in PP1 protein levels.B. Overexpression of Nox4 in H9c2 cells caused prolongation of tunicamycin-induced eIF2a phosphorylation, with minimal change in phospho-PERK levels.C,D. Mean levels of phosphorylated eIF2a relative to total eIF2a protein in tunicamycin-treated cells after Nox4 knockdown or overexpression, respectively. n=3/group. *, significant compared to baseline; #, significant comparing Nox4 knockdown (Ad.shNox4) or overexpression (Ad.Nox4) versus corresponding controls (Ad.Ctl or Ad.β-Gal, respectively)."
NCBI gene IDs:
"β-Gal: 
Nox4: 85431"
Figure legend: "E,F. Effect of Nox4 knockdown or overexpression, respectively, on okadaic acid-resistant Ser/Thr phosphatase activity in membrane fractions of tunicamycin-treated H9c2 cells. n=4/group. *p&lt;0.05, **p&lt;0.01 cf. baseline; #, p&lt;0.05, ##, p&lt;0.01 comparing Nox4 knockdown (Ad.shNox4) or overexpression (Ad.Nox4) versus corresponding controls (Ad.Ctl or Ad.β-Gal, respectively)."
NCBI gene IDs:
"β-Gal: 
Nox4: 85431"
Figure legend: "G,H. Nox4 knockdown or overexpression, respectively, had no effect on the phosphorylation of glycogen synthase at Ser641 (GS-P) or Histone H3 at Ser57 (H3-P) in H9c2 cells."
NCBI gene IDs:
"Nox4: 85431"
Figure legend: "I. Nox4-/- MEF cells (KO) showed blunted tunicamycin-induced increases in levels of phospho-eIF2a, ATF4 and ER chaperones as compared to wild-type (WT) MEFs, a response that was rescued by re-introduction of Nox4 (KO+Nox4). The latter had no effect on GS-P or H3-P levels."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "E. The association of Nox4 with GADD34 was validated in HEK293 cells co-transfected with Nox4 and either Flag-tagged or non-tagged GADD34, followed by IP with an anti-Flag antibody."
NCBI gene IDs:
"Nox4: 50507
GADD34: 17872"
Figure legend: "F. Co-transfection of HEK293 cells with GADD34-Flag and different myc-tagged Nox4 constructs, followed by IP with an anti-myc antibody. GADD34 binds to full-length Nox4 (FL) and the Nox4 transmembrane domain (TD) but not the C-terminal domain (CD)."
NCBI gene IDs:
"Nox4: 50507
GADD34: 17872"
Figure legend: "G. Representative pseudocolor images of simultaneous ER and cytosolic ROS measurement with HyPer ER and HyPerRed Cyto, respectively, in tunicamycin-treated MEF cells. Redox-insensitive mutant probes were used as negative controls and to exclude pH changes. Extracellular H2O2 (200 nM) was added as a positive control. The pseudocolor scale is shown along the left vertical edge of each image. KO = Nox4-/-. Scale bars, 2 µm."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "H. Transfection of HEK293 cells with PP1 and GADD34 increased PP1 activity (bar graph) and decreased phospho-eIF2 levels (immunoblots). Co-transfection of full-length Nox4 reduced PP1 activity and increased phospho-eIF2 levels (captions at bottom refer both to the bar graphs and immunoblots). These effects were abrogated when either Nox4 P437H or the Nox4 transmembrane domain (TD) were transfected. Nox4 did not affect phosphorylation of glycogen synthase (GS-P) or histone H3 (H3-P). All experiments were n=3/group. Values below the immunoblots are mean ± SEM levels for phospho-eIF2/total-eIF2. *, p&lt;0.05 comparing third and fourth lanes; Levels of significance for comparisons of PP1 activities are shown above the bar columns. See also Fig EV1 and Appendix Fig S4."
NCBI gene IDs:
"Nox4: 50507
PP1: 5501
GADD34: 17872"
Figure legend: "A. Recombinant PP1 was inhibited by H2O2 (0.2 mM) and activity was not restored by glutathione (GSH), cysteine (Cys) or dithiothreitol (DTT). A Cys127Ser/Cys273Ser PP1 mutant was inhibited by H2O2 similarly to wild-type PP1. Values above bars denote level of significance for the inhibitory effect of H2O2."
NCBI gene IDs:
"PP1: 5501"
Figure legend: "E. Effect of Nox4 on GADD34/PP1-mediated eIF2 dephosphorylation in transfected HEK293 cells. Nox4 increased eIF2 phosphorylation in cells transfected with GADD34 and WT PP1, and resulted in even higher phospho-eIF2α levels in cells transfected with N124D or D64N PP1 variants."
NCBI gene IDs:
"Nox4: 50507
PP1: 5501
GADD34: 17872"
Figure legend: "F. Effect of ascorbate (Asc, 0.5 mM) on phosphatase inhibition in tunicamycin-treated H9c2 cells with overexpression or knockdown of Nox4 (Ad.Nox4 and Ad.shNox4, respectively). In control cells, ascorbate enhanced tunicamycin-stimulated increases in phosphatase activity. Phosphatase activity was lower in Nox4-overexpressing than control cells but was normalized by ascorbate to the same level as in control cells. In Nox4 knockdown cells, tunicamycin-induced increases in phosphatase activity were enhanced and ascorbate had minimal additional effect."
NCBI gene IDs:
"Nox4: 85431"
Figure legend: "A. H9c2 cells treated with tunicamycin (2 µg/ml, 12h) showed significantly lower survival when endogenous Nox4 was silenced (siNox4) as compared to cells treated with a scrambled siRNA (siCtl). Cell survival was restored by treatment with either guanabenz (Gbz, 5 µM) or salubrinal (Sal, 50 µM) but was unaffected by clonidine (Cld, 5 µM). n=3/group."
NCBI gene IDs:
"Nox4: 85431"
Figure legend: "B. Nox4-depleted cells had lower levels of phospho-eIF2α and ATF4 than control cells but these were restored in the presence of guanabenz (Gbz)."
NCBI gene IDs:
"Nox4: 85431"
Figure legend: "D. Hearts from Nox4 knockout (KO) mice and WT controls were subjected to global ischemia followed by aerobic reperfusion (I/R). Infarct size assessed by triphenyltetrazolium chloride (TTC) staining was greater in Nox4 KO hearts compared to WT and was significantly reduced by guanabenz (Gbz). In the representative heart sections shown at the top, white denotes infarct area and red the viable area. Scale bars, 1 mm. Numbers of hearts are indicated within the bars."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "E. Immunoblotting of heart homogenates after I/R showed lower levels of phospho-eIF2a, ATF4 and ER chaperones, and higher levels of cleaved caspase-12, in Nox4 KO compared to WT. Tubulin was used as a loading control. Treatment with guanabenz (Gbz) reversed these changes (blots shown to the right)."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "A. Plasma urea levels were elevated to a greater extent in tunicamycin-treated Nox4 KO mice than WT. Co-treatment with guanabenz (Gbz) reduced urea levels in both groups. Numbers of animals are indicated within bars."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "B. 48 hours after systemic tunicamycin treatment, kidneys of Nox4 KO mice showed a marked surface pallor (bottom right)."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "C. TUNEL staining revealed a significantly higher number of apoptotic cells in tunicamycin-treated Nox4 KO mice. n=4/group."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "D. Immunoblotting of kidney homogenates showed significantly elevated cleaved caspase-12 and cleaved PARP levels in tunicamycin-treated Nox4 KO mice compared to WT."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "E. Survival curves showed that a very high proportion of Nox4 KO mice died after AKI. Guanabenz (Gbz) treatment dramatically improved survival in tunicamycin-treated KO mice. Number of animals as indicated. Levels of significance by Kaplan Meier analysis are reported to the right."
NCBI gene IDs:
"Nox4: 50490"
Figure legend: "Distribution of RT shifts calculated with the -ΔL+ΔE method described in Appendix Figure S1. Different transgenes in a mid-late-replicating region are compared: the IL2R  reporter gene under the control of the βA-globin promoter (βA pro) containing an inactive (i, N=10) or an active origin (ii, N=8) flanked by two copies of FIV( 2xFIV), the blasticidin resistance gene (BsR) under the control of the β-actin promoter (β-act  pro) (iii, N=7) or a combination of these two transgenes (iv, N=8). Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites respectively. Rectangle edges correspond to the 0.25 and 0.75 quartiles, the thick black lines represent the median, the white triangles represent the mean and the whiskers extend to the smallest and largest -ΔL+ΔE values. Statistical analysis was performed with Wilcoxon nonparametric two-tailed tests (ns, not significant; **p&lt;0.01; ***p&lt;0.001)."
NCBI gene IDs:
"blasticidin resistance gene: 
IL2R: 
β-act: 
BsR: 
β-actin: 
βA: 
βA-globin: "
Figure legend: "A. UCSC genome browser visualization of the mid-late insertion site of chromosome 1 (genomic positions: chr1:71,000,000-74,100,000 bp, 3.1 Mb; galGal5). RT  weighted average (WA) values for the wt and the βA-globin+β-actin cell lines are shown. Early-replicated regions (E) are represented in orange and late replicating regions (L) in blue. Annotated genes (Ref Seq genes) are represented below. The mid-late insertion site is indicated with a red arrow and dotted line."
NCBI gene IDs:
"β-actin: 
βA-globin: "
Figure legend: "B. UCSC genome browser visualization of the mid-late insertion site of chromosome 1 (genomic positions: chr1:72,450,000-72,650,000 bp; 200 kb; galGal5). Tracks of nascent strands (NS) enrichments in the four S phase fractions were represented separately (S1 to S4) for the wt and the 2x(βA-globin+β-actin) cell lines. NS enriched and depleted regions for each fraction are represented in purple and blue respectively. Single reads form SNS aligned and track of replication origins (Ori peaks) determined in (Massip et al, 2019) are reported in between. The three mid-late insertion sites 1, 2 and 3 are indicated with red arrows. Annotated genes and CpG Islands are shown below. Initiation zones  (IZ) and termination zones (TZ) are reported."
NCBI gene IDs:
"β-actin: 
βA-globin: "
Figure legend: "A. Distribution of -ΔL+ΔE values for clonal cell lines containing one advanced replicon (βA-globin+β-actin) inserted at site 1 (i, N=8) or 3 (iii, N=3) or at both sites on the same chromosome with one GFP  reporter construct composed of the GFP reporter gene under the control of the βA-globin promoter/origin linked to a 1.6 kb fragment of human  chromosome  7 (h.K7) inserted at site 2, either on the same chromosome as 1 and 3 (1+2+3), or on the other chromosome (1+3) (ii, N=5). Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites respectively. Black vertical bars represent insertion sites. Rectangle edges correspond to the 0.25 and 0.75 quartiles, the thick black lines represent the median, the white triangles represent the mean and the whiskers extend to the smallest and largest -ΔL+ΔE  values. Statistical analysis was performed with Wilcoxon nonparametric two-tailed tests (**p&lt;0.01)."
NCBI gene IDs:
"GFP: 
β-actin: 
βA-globin: "
Figure legend: "A. Distribution of -ΔL+ΔE values for clonal cell lines containing one β-actin  construct at site 1 (i, N=7) or at site 1 and 3 (ii, N=5), on the same chromosome. Data information: Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites respectively. Black vertical bars represent insertion sites. Rectangle edges correspond to the 0.25 and 0.75 quartiles, the thick black lines represent the median, the white triangles represent the mean and the whiskers extend to the smallest and largest -ΔL+ΔE values. Statistical analysis was performed with Wilcoxon nonparametric two-tailed tests (**p&lt;0.01)."
NCBI gene IDs:
"β-actin: 396526"
Figure legend: "B. Distribution of -ΔL+ΔE  values for clonal cell lines containing one βA-globin construct at site 1 (i, N=8) or at site 1 and 3 (ii, N=6), on the same chromosome. Data information: Blue and black triangles represent reactive loxP sites and recombined inactive loxP sites respectively. Black vertical bars represent insertion sites. Rectangle edges correspond to the 0.25 and 0.75 quartiles, the thick black lines represent the median, the white triangles represent the mean and the whiskers extend to the smallest and largest -ΔL+ΔE  values. Statistical analysis was performed with Wilcoxon nonparametric two-tailed tests (**p&lt;0.01)."
NCBI gene IDs:
"βA-globin: "