EMBO/SourceData huggingface dataset:
Figure legend: "A, B WT and  SNX10 KO Caco-2 cells were treated with or without  OMVs (100 μg/mL) for 24 h. The fold changes in mRNA levels of SNAI1 (encoding Snail) (A) and SNAI2  (encoding  Slug) (B) in  Caco-2  cells were determined by RT-qPCR. n = 6 independent experiments. Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ns, not significant."
NCBI gene IDs:
"SNAI1: 6615
Snail: 6615
SNAI2: 6591
Slug: 6591
SNX10: 29887"
Figure legend: "C WT and SNX10 KO Caco-2  cells were treated with or without  OMVs (100 μg/mL) for 24 h. Cytoplasmic and nuclear proteins were extracted for Snail and Slug  determination by immunoblots."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "WT and  SNX10 KO Caco-2  cells were treated with or without OMVs  (100 μg/mL) for 24 h. quantiﬁed the percentage of  Snail (E) or Slug (F)-positive nuclei respectively. Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ***p &lt; 0.001."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "H WT and SNX10  KO Caco-2  cells were transfected with  Ad-vector or  Ad-SNX10, followed by treatment with or without 100 μg/mL OMVs for 24 h. Proteins from nucleus,  cytoplasm and total cell lysates were subjected to immunoblots  with the indicated antibodies."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "C, D Levels of p-Lyn, Lyn,  E-cadherin and SNX10 in WT and SNX10 KO  Caco-2 cells treated with or without OMVs  (100 μg/mL) for 24h were determined by  immunoblots (C) and quantified by ImageJ software (D) (n = 3 independent experiments). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "E, F  Caco-2 cells were transfected with Ad-vector or Ad-SNX10, followed by treatment with or without  OMVs (100 μg/mL) for 24h. Lysates were extracted and subjected to immunoblots  with the indicated antibodies (E) and quantified by ImageJ software (F) (n = 3 independent experiments). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "I Cytoplasmic and  nuclear proteins were extracted from WT and  SNX10 KO  Caco-2 cells treated with or without Tolimidone (10 μM), and the indicated proteins were analyzed by  immunoblots."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "D WT and SNX10 KO Caco-2  cells were treated with or without  OMVs (100 μg/mL) for 24 h and then subjected to  IP  with anti-caspase-5 antibody."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "E Representative images of  co-staining of  Lyn and caspase-5 in WT and SNX10 KO Caco-2 cells treated with or without OMVs (100 μg/mL) for 24 h. Scale bar: 20 μm. F The  co-localization of  Lyn and caspase-5 was quantified by ImageJ software. n = 3 independent experiments, n = 18 fields analysed. Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ***p &lt; 0.001. "
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "H Lysates of Flag-tagged full-length SNX10 or its different truncated mutants transfected Caco-2 cells were subjected to immunoprecipitation."
NCBI gene IDs:
"Flag: 
SNX10: 29887"
Figure legend: "E, WT and SNX10 KO  Caco-2 cells were treated with  OMVs (100 μg/mL) for the indicated  time and  stained with antibodies against LPS  and Rab5 (E). Scale bar: 20 μm."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "G  LPS levels in the cell culture supernatants of WT and SNX10 KO Caco-2 cells stimulated by OMVs (100 μg/mL) for the indicated time were detected by  LAL assay (n = 6 independent experiments). H LPS levels in the  cytosolic and residual fractions of  Caco-2 cells stimulated by OMVs (100 μg/mL) with or without chloroquine (CQ) (200 nM) or bafilomycin A1 (Baf A1) (50 nM) for the indicated time were detected by LAL assay  (n = 6 independent experiments). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001; ns, not significant. "
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "C WT and SNX10 KO Caco-2 cells were treated with or without OMVs (100 μg/mL) for 24h and then subjected to IP with anti-PIKfyve antibody."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "D, E  Co-staining of PIKfyve and caspase-5 in WT and  SNX10 KO  Caco-2  cells treated with or without  OMVs (100 μg/mL) for 24 h (D). Anti-PIKfyve and anti-caspase-5 antibodies were used to detect endogenous PIKfyve and caspase-5 respectively. Scale bar: 20 μm. Colocalized PIKfyve and caspase-5 were quantified by ImageJ software (E) (n = 3 independent experiments, n = 18 fields analysed). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ***p &lt; 0.001."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "G, H Co-staining of PIKfyve  and Rab5  in WT and SNX10 KO  Caco-2 cells treated with or without OMVs (100 μg/mL) for 24 h (G). Anti-PIKfyve and anti-Rab5 antibodies were used to detect endogenous  PIKfyve and Rab5 respectively. Scale bar: 20 μm. Colocalized PIKfyve and caspase-5 were quantified by ImageJ software (H) (n = 3 independent experiments, n = 18 fields analysed). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ***p &lt; 0.001."
NCBI gene IDs:
"SNX10: 29887"
Figure legend: "I LPS levels in the  cytosolic and residual fractions of Caco-2  cells stimulated by OMVs  (100 μg/mL) with or without apilimod (30 nM) for the indicated time were detected by  LAL assay (n = 6 independent experiments). J  Caco-2 cells transfected with control siRNA or CASP5 siRNA were treated with OMVs (100 μg/mL) for the indicated time.  LPS levels in the cytosolic and residual fractions of Caco-2 cells were detected by  LAL assay (n = 6 independent experiments). K LPS levels in the cytosolic  and residual fractions of Caco-2 cells stimulated by  OMVs  (100μg/mL) with or without bafetinib (1μM) for the indicated time were detected by LAL assay (n = 6 independent experiments). Data information: Data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. ***p &lt; 0.001. "
NCBI gene IDs:
"CASP5: 838"
Figure legend: "SNX10 conditional knockout (SNX10 cKO) mice  with or without  IL-10 KO background and littermate wild type (WT) mice with or without IL-10  KO background were compared (n = 6 animals, each group). B, C Length of  colons from WT, SNX10 cKO, IL-10 KO and SNX10 cKO IL-10 KO  mice was measured (B) and analysed (C) at the 16th week. Data information: data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"IL-10: 16153
SNX10: 71982"
Figure legend: "SNX10 conditional knockout (SNX10 cKO) mice with or without IL-10 KO background and littermate wild type (WT)  mice with or without IL-10 KO background were compared (n = 6 animals, each group). E, F Representative H&amp;E images of colon tissues of WT, SNX10 cKO,  IL-10 KO and SNX10  cKO IL-10  KO mice  at the 16th week were shown (E) and analyzed (F). Scale bar: 100 μm. Data information: data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"IL-10: 16153
SNX10: 71982"
Figure legend: "SNX10 conditional knockout (SNX10 cKO)  mice with or without IL-10  KO background and littermate wild type (WT) mice with or without IL-10 KO background were compared (n = 6 animals, each group). G The content of TNF-α,  IL-6, IL-12/IL-23 p40 and IL-17A in the serum of WT, SNX10 cKO, IL-10 KO and SNX10 cKO IL-10 KO mice  at the 16th week were measured by ELISA. Data information: data are means ± SD. One-way ANOVA followed by Bonferroni post hoc test was used for statistical analyzes. *p &lt; 0.05; **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"IL-10: 16153
SNX10: 71982"
Figure legend: "SNX10 conditional knockout (SNX10 cKO) mice with or without  IL-10 KO background and littermate wild type (WT) mice with or without IL-10 KO background were compared (n = 6 animals, each group). J Protein expression of  E-cadherin, p-Lyn and Lyn in colon epithelial tissues was determined by immunoblots."
NCBI gene IDs:
"IL-10: 16153
SNX10: 71982"
Figure legend: "(C) Representative flow cytometry results showing CD71, a cell surface marker, can be efficiently knocked-down in the majority of organoid cells after 5 days of Dox  and TMP  treatment. N = 3 different organoid lines and 3 different gRNAs for CD71  were used. Mean ± SEM is labelled."
NCBI gene IDs:
"CD71: 7037"
Figure legend: "(B) qRT-PCR  showing that SOX9  gRNAs effectively knocked down SOX9  transcript levels using inducible CRISPRi after 5 days of Dox and TMP  treatment. 3 different organoid lines and 2 different SOX9  gRNAs were used. Error bars: mean ± SEM. Two-sided Student's t-test with equal variance ***p &lt; 0.001."
NCBI gene IDs:
"CRISPR: 
SOX9: 6662"
Figure legend: "(E) LGR5  mRNA expression  enrichment in SOX9   expressing  tip progenitor cells. SOX9   red, LGR5  cyan. (F) Tip progenitor cells are of high WNT signalling activity. NOTUM   yellow, SOX9   red. Data information: Scale bars = 50 μm"
NCBI gene IDs:
"LGR5: 8549"
Figure legend: "(H) qRT-PCR  showing rescue of SOX9  transcription after constitutively activated β-catenin  overexpression in organoids  cultured without WNT   activators. N = 3 different organoid lines. Error bars: mean ± SEM."
NCBI gene IDs:
"β-catenin: 1499
SOX9: 6662"
Figure legend: "(I) WB  showing SOX9  protein was rescued after constitutively activated β-catenin overexpression in organoids  cultured without WNT   activators."
NCBI gene IDs:
"β-catenin: 1499"
Figure legend: "(A,B) HCR  images showing ETV5  (A) and ETV4  (B) expression  in SOX9  expressing  tip progenitors. ETV4/5 red, SOX9  cyan. Data information: Scale bars denote 50 μm (A, B)"
NCBI gene IDs:
"ETV4: 2118
ETV5: 2119
SOX9: 6662"
Figure legend: "(C) qRT-PCR showing ETV4  and ETV5  dual knockdown in  tip organoids. N =3 different organoid lines and 2 different ETV4  and ETV5  gRNA combinations were used. Error bars: mean ± SEM. Two tailed paired t-test: **p &lt; 0.01; ***p &lt; 0.001."
NCBI gene IDs:
"ETV4: 2118
ETV5: 2119"
Figure legend: "(F) qRT-PCR of ETV4, ETV5  and SOX9 expression  level in organoids  in self-renewing medium and self-renewing medium without RTK   ligands. N = 3 different organoid lines used. Error bars: mean ± SEM. Two-tailed paired T-test: **p &lt; 0.01; n.s. non-significant."
NCBI gene IDs:
"ETV4: 2118
ETV5: 2119
SOX9: 6662"
Figure legend: "(B) Lysates from 293T  cells transfected with HA-TRIM21  and Flag-SIRT5  or vector control (-) as indicated were immunoprecipitated  (IP) using anti-Flag  antibody, and bound proteins were analyzed by  western blotting (WB). Data information: All IP  and WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
SIRT5: 23408
TRIM21: 6737"
Figure legend: "(F) Immunoblot  (IB) analysis of whole-cell lysates (WCLs) and immunoprecipitates (IPs) derived from 293T cells transfected with Flag-SIRT5  together with HA-TRIM21 or indicated HA-tagged mutant TRIM21  constructs. Cells were treated with 10 μM MG132  for 4 hours before harvesting. HA, hemagglutinin."
NCBI gene IDs:
"Flag: 
HA: 
SIRT5: 23408
TRIM21: 6737"
Figure legend: "(G) IB  analysis of WCLs derived from 293T  cells transfected with increasing amounts of Flag-TRIM21  construct. Where indicated, 10 μM MG132  was added for 4 hours before harvesting."
NCBI gene IDs:
"Flag: 
TRIM21: 6737"
Figure legend: "(H) 293T  cells transfected with control or TRIM21  siRNA for 48 hours were then treated with 100 ng/mL CHX  for the indicated time  period before harvesting. Protein expression was analyzed by  western blotting (left panel). The SIRT5  protein abundance was quantified by ImageJ and plotted as indicated (right panel). Data information: All WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"TRIM21: 6737"
Figure legend: "(I) IB  analysis of WCLs derived from TRIM21+/+ and TRIM21-/- BMDMs  treated with 100 ng/ml CHX  for the indicated time  period  before harvesting (left panel). The SIRT5  protein abundance was quantified (right panel)."
NCBI gene IDs:
"TRIM21: 20821"
Figure legend: "(J) IB  analysis of WCLs derived from TRIM21+/+ and TRIM21-/- BMDMs treated with 100 ng/mL LPS  for the indicated time  period before harvesting (top panel). The SIRT5  protein abundance was quantified (bottom panel)."
NCBI gene IDs:
"TRIM21: 20821"
Figure legend: "(B) WCLs from TRIM21+/+ and TRIM21-/- BMDMs  treated with 100 ng/mL LPS  for 4 hours were immunoprecipitated  with anti-SIRT5  antibody and analyzed by western blotting. 10 μM MG132  was added for 4 hours before harvesting. Data information: All IP  and WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"TRIM21: 20821"
Figure legend: "(E) WCLs from TRIM21+/+ and TRIM21-/- BMDMs  treated with 100 ng/mL LPS  for 4 hours were immunoprecipitated  with anti-SIRT5  antibody and analyzed by  western blotting using antibodies specifically against Ub-K48 and Ub-K63. 10 μM MG132  was added for 4 hours before harvesting. Data information: All IP  and WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"TRIM21: 20821"
Figure legend: "(A) Lysates from 293T  cells transfected with HA-HAUSP  and Flag-SIRT5  or vector control (-) as indicated were immunoprecipitated  (IP) using anti-Flag  antibody, and bound proteins were analyzed by  western blotting (WB). Data information: All IP  and WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"Flag: 
HA: 
SIRT5: 23408
HAUSP: 7874"
Figure legend: "(E) 293T  cells were transfected with Flag-tagged HAUSP, mutant HAUSP-CS (C223S) or vector control as indicated for 48 hours. protein expression was determined by western blot  analysis using indicated antibodies. Data information: All WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"Flag: 
HAUSP: 7874"
Figure legend: "(F) IB  analysis of WCLs derived from 293T  cells transfected with HA-SIRT5  together with Flag-HAUSP  or vector control constructs (left panel). Cells were treated with 100 ng/ml CHX  for the indicated time  period  prior to harvesting. The SIRT5  protein abundance was quantified (right panel)."
NCBI gene IDs:
"Flag: 
HA: 
SIRT5: 23408
HAUSP: 7874"
Figure legend: "(F) IB  analysis of WCLs and anti-SIRT5 immunoprecipitated  products derived from BMDMs  transfected with control or HAUSP  siRNA for 48 hours. 10 μM MG132  was added for 6 hours before harvesting."
NCBI gene IDs:
"HAUSP: 252870"
Figure legend: "(G) BMDMs  transfected with control (-) or HAUSP  siRNA for 48 hours were then treated with 100 ng/mL mM LPS  for 4 hours, and 10 μM MG132  was added for 4 hours before harvesting. WCLs and anti-SIRT5 immunoprecipitated  products were prepared and subjected to IB  analysis using indicated antibodies."
NCBI gene IDs:
"HAUSP: 252870"
Figure legend: "(B) IB  analysis of WCLs and anti-TRIM21 immunoprecipitated  products derived from 293T  cells transfected with control or SIRT5  siRNA using anti-Flag, anti-acetyl Lysine (AcK), anti-succinyl Lysine (SulK), anti-glutaryl Lysine (GluK) and anti-malonyl Lysine (MalK) antibodies."
NCBI gene IDs:
"SIRT5: 23408"
Figure legend: "(E) Protein expression in SIRT5+/+ and SIRT5-/- BMDMs  was determined by  western blot  analysis. Data information: All WB  data in this work otherwise indicated are representative of at least three independent experiments."
NCBI gene IDs:
"SIRT5: 68346"
Figure legend: "(E) Genotyping of TRIM21-/-, SIRT5-/- and TRIM21-/-SIRT5-/- mice. DNA derived from the tail of wild type and knockout animals was analyzed by PCR. Protein expression of TRIM21  and SIRT5  in BMDMs  derived from mice  were also analyzed by  western blotting. Data information: Western blots in E represent three independent experiments."
NCBI gene IDs:
"SIRT5: 68346
TRIM21: 20821"
Figure legend: "wild-type (WT, n=5), TRIM21-/- (n=5), SIRT5-/-(n=5), and TRIM21-/-SIRT5-/- (n=5) mice  were orally administrated with 2.5% (w/v) DSS  in drinking water for 7 days and regular drinking water for another 2 days. Colon-length (G) changes were measured. Data information: Data are means ± SD. n=5 biological replicates. P values were determined by unpaired two-tailed Student's t-tests. ns, not significant; ***p &lt; 0.001, ****p &lt; 0.0001."
NCBI gene IDs:
"SIRT5: 68346
TRIM21: 20821"
Figure legend: "(A-D) Immunostaining  with dopamine  antibody at late 3rd instar  (96h AEL) in control background (domeMESO-GFP/+). The inset shows DomeGFP+ cells (B') and the corresponding dopamine  levels (C') in them. (E-L) Similar expression  analysis of dopamine  at early 3rd (72h AEL, E-H) and early 2nd instar (48h AEL, I-L). (D, H and L) The spectral mode of iDopamine  representation with blue pixels indicating low and red pixels indicating high intensity. (M) Quantifications of dopamine  levels in DomeGFP+ progenitors  (domeMESO-Gal4, UAS-GFP/+, n= 14 at 48h, n=13 at 72h and n=16 at 96h AEL). Data information: DNA  is stained  with Hoechst  in blue, intracellular Dopamine  (iDopamine) in red, domeMESO expression  in green (representative of progenitors  in the  medullary zone, MZ). The iDopamine channel has been converted to spectral mode in panels D, H and L. All image panels show a 20µm scale bar. The inset represents a 2X zoom of region of interest (ROIs) marked in white dashed square. AEL indicates After Egg Laying. For better representation, the primary lobe  of the lymph gland  has been represented and outlined in white dashed lines while the MZ  is outlined in yellow dashed line for segregation between Dome+ and Dome- cells. The quantification in M shows median with whiskers indicating the maximum and minimum values,. box indicates the lower and upper quartiles. The statistical analysis applied is One-way ANOVA with Tukey's multiple comparisons test. 'n' represents the number of  lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001. For better representation, the primary lobe  of the  lymph gland has been represented and outlined in white dashed lines while the MZ  is outlined in yellow dashed line."
NCBI gene IDs:
"GFP: 
dome: 32976
Gal4: 855828"
Figure legend: "(B-D') A temporal expression  profile of TH  at 48, 72 and 96h AEL lymph glands in control background (domeMESO-Gal4, UAS-GFP/+) (E-G') A temporal expression  profile of Ddc  at 48, 72 and 96h AEL lymph glands in control background (domeMESO-Gal4, UAS-GFP/+). (H) Quantification of TH  levels (domeMESO-Gal4, UAS-GFP/+, n=18 at 48h, n= 15, at 72h and n= 23 at 96h AEL). (I) Quantification of Ddc  levels (domeMESO-Gal4, UAS-GFP/+, n= 17 at 48h, n= 33 at 72h and n= 38 at 96h AEL). Data information: DNA  is stained  with Hoechst  in blue, iDopamine in red, Tyrosine hydroxylase (TH) and Dopa decarboxylase  (Ddc) in red, domeMESO (representative of progenitors) expression  in green, AEL indicates After Egg Laying. The quantifications in H, I represents the median with whiskers indicating maximum and minimum values, box indicates the lower and upper quartiles. The statistical analysis applied in H, I, is One-way ANOVA with Tukey's multiple comparisons test 'n' represents the number of lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001. For better representation, the primary lobe  of the lymph gland has been represented and outlined in white dashed lines while the MZ  is outlined in yellow dashed line."
NCBI gene IDs:
"GFP: 
dome: 32976
Gal4: 855828"
Figure legend: "(J-L) Assessment of dopamine  levels upon the knockdown of TH  and Ddc  in the progenitors  using domeMESO-Gal4, UAS-GFP. (M) The mean intensity quantification of dopamine  levels upon TH  and Ddc  loss of function in the progenitors  (domeMESO-Gal4, UAS-GFP/+, n=40, domeMESO-Gal4, UAS-GFP; THRNAi, n= 17, domeMESO-Gal4, UAS-GFP; DdcRNAi, n= 34). Data information: DNA  is stained   with Hoechst  in blue, iDopamine in red All image panels show a 20µm scale bar. The quantifications in M represents the median with whiskers indicating maximum and minimum values, box indicates the lower and upper quartiles. The statistical analysis applied in M, is One-way ANOVA with Tukey's multiple comparisons test 'n' represents the number of  lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001. For better representation, the primary lobe  of the lymph gland  has been represented and outlined in white dashed lines"
NCBI gene IDs:
"GFP: 
Ddc: 35190
dome: 32976
Gal4: 855828
TH: 38746"
Figure legend: "(N-P) The loss of progenitor TH  and Ddc  function using domeMESO-Gal4, UAS-GFP  analysed at wandering 3rd instar, 120h AEL. Data information: DNA  is stained  with Hoechst  in blue, domeMESO  (representative of progenitors) expression  in green, P1 (representative of mature plasmatocytes) in red. All image panels show a 20µm scale bar. AEL indicates After Egg Laying. For better representation, the primary lobe  of the lymph gland has been represented and outlined in white dashed lines"
NCBI gene IDs:
"GFP: 
Ddc: 35190
dome: 32976
Gal4: 855828
TH: 38746"
Figure legend: "(Q) Quantifications of  lymph gland  size upon loss of TH  (domeMESO-Gal4, UAS-GFP; THRNAi, n= 34) and Ddc  (domeMESO-Gal4, UAS-GFP; DdcRNAi, n= 56) when compared to stage matched control (domeMESO-Gal4, UAS-GFP/+, n=90). Data information: The quantifications in Q represent mean with standard deviation with whiskers indicating maximum and minimum values, box indicates the lower and upper quartiles. The statistical analysis applied in is One-way ANOVA with Tukey's multiple comparisons test 'n' represents the number of lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001."
NCBI gene IDs:
"GFP: 
Ddc: 35190
dome: 32976
Gal4: 855828
TH: 38746"
Figure legend: "(R) Quantifications of the proportions of %Dome (green bars), %negative (blue bar), %overlap (Dome  and P1, yellow bars) and %P1  (red bars). For Dome  positive area (domeMESO-Gal4, UAS-GFP/+, n=27, domeMESO-Gal4, UAS-GFP; THRNAi, n= 15 and domeMESO-Gal4, UAS-GFP; DdcRNAi, n= 18), negative and overlap areas (domeMESO-Gal4, UAS-GFP; THRNAi, n= 15, domeMESO-Gal4, UAS-GFP; DdcRNAi, n= 18) and P1  positive area (domeMESO-Gal4, UAS-GFP/+, n=27, domeMESO-Gal4, UAS-GFP;  THRNAi, n= 15 and domeMESO-Gal4, UAS-GFP;  DdcRNAi, n= 18). Data information: The quantifications in R represents mean with whiskers indicating maximum and minimum values, box indicates the lower and upper quartiles. The statistical analysis applied in R is Two-way Anova with Tukey's multiple comparisons test. 'n' represents the number of  lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001."
NCBI gene IDs:
"GFP: 
Ddc: 35190
dome: 32976
Gal4: 855828
TH: 38746"
Figure legend: "(S) Quantification of the number of PPO  positive crystal cells upon dopamine  synthesis perturbation (domeMESO-Gal4, UAS-GFP/+, n=29, domeMESO-Gal4, UAS-GFP; THRNAi, n= 21, domeMESO-Gal4, UAS-GFP; DdcRNAi, n= 23). Data information: The quantifications in S represent mean with standard deviation with whiskers indicating maximum and minimum values, box indicates the lower and upper quartiles. The statistical analysis applied in S is One-way ANOVA with Tukey's multiple comparisons test 'n' represents the number of lymph gland lobes  analyzed, *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001."
NCBI gene IDs:
"GFP: 
Ddc: 35190
dome: 32976
Gal4: 855828
TH: 38746"