EMBO/SourceData huggingface dataset:
Figure legend: "Pooled clones (A-D) and single cell clones (E-H) of HEK293/sw cells untransfected or stably transfected with the indicated WT and mutant PS1 constructs were analyzed for γ-secretase expression and APP processing.(G) Secreted Aβ was analyzed as in (D). To verify individual Aβ species, Aβ standards were co-migrated. Conditioned media were analyzed for individual Aβ species on Tris-Bicine urea SDS-PAGE by combined immunoprecipitation/immunoblotting of Aβ using antibodies 3552/2D8."
NCBI gene IDs:
"PS1: 5663"
Figure legend: "Pooled clones (A-D) and single cell clones (E-H) of HEK293/sw cells untransfected or stably transfected with the indicated WT and mutant PS1 constructs were analyzed for γ-secretase expression and APP processing.(H) Total Aβ in conditioned media was analyzed by MALDI-TOF MS following immunoprecipitation with antibody 4G8. Observed (Aβ42, 4513.6; Aβ43, 4615.3) and predicted molecular masses (Aβ42, 4514.1; Aβ43, 4615.2) were in good agreement."
NCBI gene IDs:
"PS1: 5663"
Figure legend: "(A) PS1/2-/- MEF cells stably transduced with PS1 WT or PS1 L435F were analyzed for PS1 expression and APP processing by immunoblotting as in Fig 1A. Antibody 5E12 was used for the detection of the PS1 CTF."
NCBI gene IDs:
"PS1: 19164
PS1: 5663"
Figure legend: "(B) Stably transduced PS1/2-/- MEF cells of A were transiently transfected with APPsw-6myc and conditioned media were analyzed for Aβ species by Tris-Bicine urea SDS-PAGE. Individual Aβ species were verified by co-migration with Aβ standards (left panel) and quantified (right panel). Data represent mean ± s.e.m. of n=3 independently performed transfections. Note that more sample was loaded for the PS1 L435F mutant to facilitate immunoblot analysis."
NCBI gene IDs:
"APP: 351
PS1: 5663
PS1: 19164"
Figure legend: "(C) Secreted Aβ from cells in B were quantified by ELISA. Data represent mean ± s.e.m. of n=5 (PS1 WT) or 6 (PS1 L435F) independently performed transfections."
NCBI gene IDs:
"PS1: 5663"
Figure legend: "Immunohistochemical detection of Aβ40 (left column), Aβ42 (medium column) and Aβ43 (right column) in consecutive frontal cortex paraffin sections of two FAD cases with PS1 L435F mutation (cases #1 and #2, 1st and 2nd rows), another FAD case with different PS1 mutation (case #3, 3rd row) and one SAD case (case #4, forth row). In both PS1 L435F mutation cases the numerous Aβ plaques mainly contain Aβ42 and Aβ43 but less Aβ40. This is in contrast to Aβ plaques of the control FAD and SAD cases, in which solely Aβ42 predominates and Aβ43 is sparse; even levels of Aβ40 seem to be lower than in the PS1 L435F cases. Note that in both PS1 L435F cases several plaques are larger than those seen in cases #3 and #4, representing cotton wool plaques. Scale bar = 500 µm. Magnification is identical in all pictures."
NCBI gene IDs:
"PS1: 5663"
Figure legend: "  HCT-116 cells were transfected with BIP or CHOP specific siRNAs for 48 h and then treated with or without 2mM sodium butyrate (NaB) for 24 h. Negative control (NC) scramble siRNA was used the negative control for the transfection. Representative Western blots are shown for BIP siRNA (A) and CHOP siRNA (C). The expression level of each protein was determined by densitometry and normalized to GAPDH (ratio of protein:GAPDH). (B) Normalized expression levels of BIP and LC3-II in HCT-116 cells treated with BIP specific siRNA. (C) Normalized expression levels of CHOP and LC3-II in HCT-116 cells treated with CHOP specific siRNA. Means and standard deviation (SD) of three independent experiments are shown. One-way ANOVA was used for statistical analysis. * P&lt;0.05, ** p&lt;0.01, compared to the respective control group.  "
NCBI gene IDs:
"CHOP: 1649
BIP: 64374"
Figure legend: "HA immunoprecipitation of stably expressed ZNF282-HA in cells previously transfected with ΔDUF3669-ZNF398-GFP and WT-ZNF398-GFP followed by detection of ZNF398 constructs in the IPs through Western-Blot using an anti-GFP antibody. Bottom: Western-Blot using an anti-HA antibody on the IPs. Input = Cellular lysate, IP = Immunoprecipitate"
NCBI gene IDs:
"GFP: 
ZNF398: 57541"
Figure legend: "Left: DUF3669-only and KRAB domain constructs used. Right: HA immunoprecipitation in cells previously co-transfected with ZNF398-DUF3669-HA and either ZNF282-KRAB-Flag or ZNF282-DUF3669-Flag followed by detection of either of these protein constructs in the IPs through Western-Blot using an anti-Flag antibody. Bottom: Western-Blot using an anti-HA antibody on the IPs."
NCBI gene IDs:
"Flag: 
HA: 
ZNF282: 8427
ZNF398: 57541"
Figure legend: "Immunoprecipitations of HA-tagged KRAB domains in order to check\u2028 interaction with cKAP1. Co-transfection of Flag-tagged cKAP1 (upper panel) or Flag-tagged hKAP1 (lower panel) and HA-tagged ZNF398 DUF3669 domain negative control, H2ZYM4 and M3XKG1 cKRAB domains and ZNF93 hKRAB domain in KAP1 KO HAP1 cells followed by HA-immunoprecipitation. cKAP1 and hKAP1 presence was revealed by Western-Blot using an anti-Flag antibody. Input = Cellular lysate, IP = Immunoprecipitate. Western-Blot using an HA antibody on the IPs at the bottom."
NCBI gene IDs:
"Flag: 
HA: 
cKAP1: 102365962
hKAP1: 10155
KAP1: 10155
ZNF398: 57541
ZNF93: 81931"
Figure legend: "Quantification of α-MHC transcript abundance N=4 to 18 mice/group, all 6 weeks after TAC or sham surgery."
NCBI gene IDs:
"α-MHC: 17888"
Figure legend: "Quantification of β-MHC transcript abundance N=4 to 18 mice/group, all 6 weeks after TAC or sham surgery."
NCBI gene IDs:
"β-MHC: 140781"
Figure legend: "(P) Western blot analysis for TIP30 and GAPDH in hearts from TIP30 wild-type (WT) and heterozygous (Het) after AAV-TropT-TIP30 or AAV-Control (AAV-Con) injection followed by 6 weeks of TAC surgery."
NCBI gene IDs:
"TIP30: 53415
TropT: 21956"
Figure legend: "Quantification of HW/TL ratio (Q) ratio in AAV-Con or AAV-TropT-TIP30 treated Tip30 heterozygous (Het) or WT mice 6 weeks after TAC or sham surgery (N=5 to 11 mice/group)."
NCBI gene IDs:
"TropT: 
Tip30: 53415
TIP30: 53415"
Figure legend: "Quantification of LuW/TL (R) ratio in AAV-Con or AAV-TropT-TIP30 treated Tip30 heterozygous (Het) or WT mice 6 weeks after TAC or sham surgery (N=5 to 11 mice/group)."
NCBI gene IDs:
"TropT: 
Tip30: 53415
TIP30: 53415"
Figure legend: "(A) Western blot for TIP30 and GAPDH in neonatal rat cardiomyocytes (NRCM) after adenoviral transduction with Ad.Control (Ad.Con) or Ad.TIP30."
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "Quantification of cardiomyocyte area (B), N=6 to 8 samples/group in NRCM transduced with Ad.Con or Ad.TIP30 and stimulated as indicated. ET-1: endothelin-1, FBS: fetal bovine serum, PE: phenylephrine."
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "Quantification of protein/DNA ratio (C), N=9 samples/group in NRCM transduced with Ad.Con or Ad.TIP30 and stimulated as indicated. PE: phenylephrine."
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "Quantification of Acta1 mRNA transcript abundance (D), N=3 samples/group in NRCM transduced with Ad.Con or Ad.TIP30 and stimulated as indicated. ET-1: endothelin-1 PE: phenylephrine."
NCBI gene IDs:
"Acta1: 29437
TIP30: 292935"
Figure legend: "Quantification of cell death with a 7-AAD assay (E), N=7 samples/group in NRCM transduced with Ad.Con or Ad.TIP30 and stimulated as indicated. PE: phenylephrine."
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "(G) Western blot for TIP30 and Actin in mouse hearts with AAV9 mediated overexpression of TIP30 (AAV-TIP30) or from mice treated with a control AAV9 construct (AAV-Con) followed by 2 weeks of TAC surgery."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "Quantification of HW/TL ratio (H), N=8 to 13 mice/group 2 weeks after sham or TAC surgery in AAV-Con or AAV-TIP30 treated C57BL/6 WT mice. *"
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "Quantification of cardiomyocyte area (I), N=4 to 5 mice/group 2 weeks after sham or TAC surgery in AAV-Con or AAV-TIP30 treated C57BL/6 WT mice."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "(J) Serial echocardiography with quantification of echocardiographic fractional area change 2, 4 and 6 weeks after sham or TAC surgery in AAV-Con or AAV-TIP30 treated C57BL/6 WT mice (N=10 to 14 mice/group and time point)."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "(B) Western blot analysis of co-immunoprecipitation (IP) from HEK cells transfected with GST-TIP30 and eEF1A1-Myc."
NCBI gene IDs:
"GST: 
Myc: 
eEF1A1: 171361
TIP30: 292935"
Figure legend: "Western blot analysis of GST-pulldown assays with GST or GST-tagged eEF1A1 (GST-eEF1A1) and TIP30-His full length protein (TIP30-His) and TIP30 deletion mutants ΔN25 (TIP30-ΔN25-His), ΔN52 (TIP30-ΔN52-His), ΔC15 (TIP30-ΔC15-His) and Δ102-107 (TIP30-ΔN102-104-His, G)"
NCBI gene IDs:
"His: 
TIP30: 292935"
Figure legend: "(J) Quantitative Real-Time PCR analysis of eEF1A1 and eEF1A2 mRNA abundance in hearts from neonatal mice, adult wild-type mice (Adult) and adult wild-type mice 2 weeks after TAC surgery (Adult TAC, N=3 to 4 mice/group). *P&lt;0.05, ***P&lt;0.001. One-way ANOVA with Sidak´s multiple comparisons test."
NCBI gene IDs:
"eEF1A1: 13627
eEF1A2: 13628"
Figure legend: "(B) Western blot analysis of anti-Myc immunoprecipitation (IP) in NRCM co-transduced with Ad.eEF1A1-Myc and either control virus (Ad.con) or Ad.TIP30. Endogenous eEF1B2 was detected. The IP input for eEF1B2, eEF1A1-myc and TIP30 is shown below. (C) Quantification of eEF1B2 abundance after eEF1A1-myc IP under conditions shown in (B) "
NCBI gene IDs:
"Myc: 
eEF1A1: 171361
TIP30: 292935"
Figure legend: "(D) Microscopy images of NRCM after adenoviral transduction with Ad.TIP30 or control virus (Ad.Con) and stimulation with phenylephrine (PE) and subsequent Proximity Ligation Assay (PLA). Red: eEF1A1-eEF1B2 interaction; Blue: DAPI. (scale bar: 50µm). (E) Quantification of eEF1A1-eEF1B2 interaction in conditions described in (D) "
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "Western blot analysis for TIP30, eEF1A1 and GAPDH in C57BL/6 WT mice 3 days (A, B), N=4 mice/group, 2 weeks (C, D), N=4 to 8 mice/group and 6 weeks (E, F), N=4 to 8 mice/group after TAC or sham surgery and their quantification. KO denotes TIP30 homozygous knock-out."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "(G, H) Western blot for TIP30, eEF1A1 and Actin in TIP30 Het mice 6 after TAC surgery and their quantification (N=4 mice/group)."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "(I, J) Western blot for TIP30, eEF1A1 and Actin 2 weeks after sham or TAC surgery in AAV-Con or AAV-TIP30 treated C57BL/6 WT mice and their quantification (N=4 mice/group). A ratio of TIP30 and eEF1A1 expression was calculated for each condition."
NCBI gene IDs:
"TIP30: 53415"
Figure legend: "Quantification of Tip30 and eEF1A1 mRNA transcript abundance in human failing hearts (A), N=6 to 8 hearts/group A ratio of Tip30 and eEF1A1 expression was calculated"
NCBI gene IDs:
"eEF1A1: 1915
Tip30: 10553"
Figure legend: "Quantification of Tip30 and eEF1A1 mRNA transcript abundance in patients with Hypertrophic Cardiomyopathy (HCM; B, N=4 to 8 hearts/group) A ratio of Tip30 and eEF1A1 expression was calculated"
NCBI gene IDs:
"eEF1A1: 1915
Tip30: 10553"
Figure legend: "Quantification of Tip30 and eEF1A1 mRNA transcript abundance in 6 months old mdx mice or WT mice (C), N=4 mice/group. A ratio of Tip30 and eEF1A1 expression was calculated"
NCBI gene IDs:
"mdx: 13405
eEF1A1: 13627
Tip30: 53415"
Figure legend: "(E) Echocardiographic diastolic left-ventricular wall-thickness in MDX mice at the age of 2 months at baseline, and 1-9 months (mo) after injection of AAV9-Con or AAV9-TIP30 (TIP; N=6 to 7 mice/group)."
NCBI gene IDs:
"MDX: 13405
TIP: 53415
TIP30: 53415"
Figure legend: "(F) Quantification of HW/TL ratio of 9mo old MDX mice, N=5 to 6 mice/group."
NCBI gene IDs:
"MDX: 13405"
Figure legend: "(A) Western blot analysis of isolated neonatal rat cardiomyocytes (NRCM) after adenoviral transduction either with control adenovirus (Con) or Ad.TIP30 (TIP30) followed by stimulation with phenylephrine (PE, for 3 hours) and puromycin incorporation (for 30 min). (B) Quantification of the Western blot shown in (A) (N=3 samples/group). "
NCBI gene IDs:
"TIP30: 292935"
Figure legend: "(C) Quantification of cell surface area of isolated neonatal mouse cardiomyocyte of Tip30 Het and WT mice treated with endothelin-1 (ET-1) and Narciclasine (Narci) or without stimulation as indicated (N=6 samples/group)."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "Quantification of HW/TL ratio (E), N=6 to 10 mice/group, in Tip30 Het or WT mice 2 weeks after TAC. Animals were treated with Narciclasine daily for 14 days after TAC as indicated."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "Quantification of , echocardiographic wall thickness (F), N=6 to 10 mice/group, in Tip30 Het or WT mice 2 weeks after TAC. Animals were treated with Narciclasine daily for 14 days after TAC as indicated."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "Quantification of cardiomyocyte area (G), N=5 mice/group in Tip30 Het or WT mice 2 weeks after TAC. Animals were treated with Narciclasine daily for 14 days after TAC as indicated."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "Quantification of fractional area change (FAC, H, N=6 to 10 mice/group) in Tip30 Het or WT mice 2 weeks after TAC. Animals were treated with Narciclasine daily for 14 days after TAC as indicated."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "(I) Western blot analysis of puromycin incorporation in hearts of Tip30 Het and WT mice 3 days after TAC or sham surgery and daily Narciclasine (Narci) injection and their quantification (N=2 to 4 mice/group). Puromycin was injected 3 hours prior to sacrifice."
NCBI gene IDs:
"Tip30: 53415"
Figure legend: "(D) mIMCD-3 cells stably expressing cilia-bPAC  or cyto-bPAC, cultured in a 3D matrix in the dark, during light  exposure (1 h light/1 h dark, 9 days, 465 nm, 38.8 µW/cm²) or in the dark  and incubated with 10 µM of Forskolin. Exemplary images are shown (n &gt; 3). (E) Quantification of the data exemplified in (D). Data are shown as mean ± S.D., each datapoint corresponds to an independent experiment; p values calculated using an unpaired, two-sided Student's t-test are indicated."
NCBI gene IDs:
"bPAC: "
Figure legend: "(D) Immunoblotting  of lysates from mIMCD-3 wild-type (WT) cells and mIMCD-3  cells stably expressing cilia-bPAC  for S6 ribosomal protein (S6RP) phosphorylation  at  Ser235/236 (pS6RP). Levels of total S6RP  protein (S6RP) and beta-Tubulin  have been determined as controls. Cilia-bPAC  cells were treated with DMSO  (control) or with 10 nM of Rapamycin. All cells were stimulated with light (1 h light/1 h dark, 48 h, 465 nm, 38.8 µW/cm²) (E) Quantification of the pS6RP/S6RP  ratio. Data are shown as mean ± S.D., n &gt; 3; p values were calculated using a paired, two-sided Student's t-test. Each data point shows an independent experiment"
NCBI gene IDs:
"bPAC: "
Figure legend: "(G) mIMCD-3 cells stably expressing cilia-bPAC, cultured in a 3D matrix in the dark, during light  exposure (1 h light/1 h dark, 9 days, 465 nm, 38.8 µW/cm²) and incubation with 10 nM rapamycin. Light  exposure started 2 d later than incubation with 10 nM rapamycin. Exemplary images are shown (n = 3) (H) Quantification of the data exemplified in (G). Data are shown as mean ± S.D., each datapoint corresponds to an independent experiment; p values calculated using a paired, two-sided Student's t-test are indicated"
NCBI gene IDs:
"bPAC: "
Figure legend: "(A)  mIMCD-3  cells stably expressing cilia-bPAC, cultured in a 3D matrix in the dark  or during light  exposure (1 h light/1 h dark, 9 days, 465 nm, 38.8 µW/cm²) and incubated with DMSO  (control), 250 µM IBMX, a ubiquitous PDE inhibitor, or 10 µM Rolipram, a PDE4-specific inhibitor. Exemplary images are shown (n = 3). Scale bars are indicated. (B) Quantification of data exemplified in (A). Data are shown as mean ± S.D., p values calculated using an unpaired, two-sided Student's t-test are indicated. Data points show individual experiments."
NCBI gene IDs:
"bPAC: "
Figure legend: "(C)  mIMCD-3  cells stably expressing cyto-bPAC, cultured in a 3D matrix during light  exposure (1 h light/1 h dark, 9 days, 465 nm, 38.8 µW/cm², 1 day after pharmacological stimulus) and incubation with DMSO  or 10 µM rolipram. Exemplary images are shown (n = 4-5). Scale bars are indicated. (D) Quantification of data exemplified in (C) Data are shown as mean ± S.D., p values calculated using an unpaired Student's t-test are indicated. Data points show individual experiments."
NCBI gene IDs:
"bPAC: "
Figure legend: "(H)  mIMCD-3 cells stably expressing cilia-bPAC, cultured in a 3D matrix during light  exposure (1 h light/1 h dark, 9 days, 465 nm, 38.8 µW/cm², 1 day after pharmacological stimulus) and incubation with DMSO  (0 µM), 3 µM, or 10 µM of the Pde4-long-isoform activator MR-L8. Exemplary images are shown (n = 3). Scale bars are indicated. (I) Quantification of data exemplified in (H). Data are shown as mean ± S.D., p values calculated using a paired, two-sided Student's t-test are indicated. Data have been normalized to the Light, 0 µM  MR-L8 condition (Set to 100%) within each experiment. Data points show individual experiments."
NCBI gene IDs:
"bPAC: "
Figure legend: "(I) 3D culture of mIMCD-3 cells stably expressing cilia-bPAC, incubated with DMSO  (control) or the EP4-inhibitor L161,982 in the dark  or during light  exposure (1 light/1 h dark, 9 days, 465 nm, 38.8 µW/cm², started 2 d after pre-incubation with DMSO  or L161,982). Exemplary images are shown (n = 3). (J) Quantification of images exemplified in (I). Data are shown as mean ± S.D., p values calculated using a paired, two-sided Student's t-test are indicated. Data points show individual experiments and have been normalized to the light/DMSO  condition (Set to 100%) within each experiment."
NCBI gene IDs:
"bPAC: "
Figure legend: "(F) 3D culture of mIMCD-3 cells stably expressing cilia-bPAC, incubated with DMSO  (control) or 20 µM Celecoxib, a COX-2-inhibitor, in the dark  or during light  exposure (1 light/1 h dark, 9 days, 465 nm, 38.8 µW/cm², started 2 d after pre-incubation with DMSO  or Celecoxib). Exemplary images are shown (n = 3). Scale bars are indicated. (G) Quantification of images exemplified in (F) and from 3D cultures incubated with 1 μM celecoxib. Data are shown as mean ± S.D., p values calculated using a paired, two-sided Student's t-test are indicated. Data points show individual experiments."
NCBI gene IDs:
"bPAC: "
Figure legend: "(A) Wild-type (WT), cilia-bPAC, or cyto-bPAC  (magenta)  mIMCD-3  cells were stimulated by light  (for 1 h prior to fixation, 465 nm, 38.8 µW/cm²), and treated with DMSO  (control) or 50 µM Ciliobrevin-D, a dynein-inhibitor. Cells were labeled with DAPI  (blue) to label the DNA, an ARL13B  antibody (white) to label cilia, and a phospho-specific (Ser133) CREB  antibody (pCREB, green). Arrows indicate the direction and the length of the shift of the respective fluorescence channel. The box indicates the position of the magnified view shown on the right of each panel. B) Quantification of the ciliary  pCREB signal in cilia-bPAC and cyto-bPAC  cells. Data are shown as mean ± S.D., n &gt; 3. Each data point represents an independent experiment and corresponds to the median of &gt; 73 cilia  (biological replicates); p values for an unpaired, two-sided Student's t-test with Welch's correction are indicated."
NCBI gene IDs:
"bPAC: "
Figure legend: "(C) mIMCD-3 Ift27-/- cells with or without stable cilia-bPAC  expression (magenta) were stimulated with light  (for 1 h prior to fixation, 465 nm, 38.8 µW/cm²). Cells were labeled with DAPI  (blue) to label the DNA, an ARL13B  antibody (white) to label cilia, and a phospho-specific (Ser133) CREB  antibody (pCREB, green). Arrows indicate the direction and the length of the shift of the respective fluorescence channel. The box indicates the position of the magnified view shown on the right of each panel. (D) Quantification of the ciliary  pCREB  signal. Data are shown as mean ± S.D., n &gt; 3. Each data point represents the median of &gt; 90 cilia  from the same experiment; p values are indicated for an unpaired, two-sided Student's t-test with Welch's correction."
NCBI gene IDs:
"bPAC: 
Ift27: 67042"
Figure legend: "(A-C) Tissue lysates from (A) spleen, (B) liver  and (C) kidney  from 6- to 9-day old indicated mice  were immunoblotted  to determine cleavage of  caspase-1. Each lane represents a lysate from a different mouse. Positive control lanes (+) are BMDMs  stimulated with LPS/ATP; negative control lanes (-) are respective organ homogenates from caspase-1-/- mice."
NCBI gene IDs:
"caspase-1: 12362"
Figure legend: "(D-G) Tissue lysates from (D, F) spleen  and (E, G) liver  from 6- to 9-day old indicated mice  were immunoblotted  to determine pro-IL-1β cleavage to  IL-1β (D-E) or GSDMD  cleavage to its p30   and p20  fragments (F-G). Each lane represents a lysate from a different mouse. Positive control lanes (+) are BMDMs  stimulated with LPS/ATP; negative control lanes (-) are respective organ homogenates from (D-E) caspase-1-/- or (F-G) Gsdmd-/- mice."
NCBI gene IDs:
"caspase-1: 12362
Gsdmd: 69146"
Figure legend: "Genotypes are indicated as WT for S100a4cre-/- x Fstl1flox+/flox+ mice and cfKO for S100a4cre+/- x Fstl1flox+/flox+. (A) S100a4 expression is induced in the ischemic area (IA). qPCR analysis of mRNA expression of S100a4 in ischemic heart and sham operated heart of WT mice and cfKO mice. Heart samples were harvested at 7 days after the surgery. Statistical analysis was performed by two-way ANOVA. Post Hoc test was performed by Tukey test. Error bars represent mean ± SEM (n=16 and 15 for WT and cfKO sham group, n=15 and 14 for WT and cfKO MI group, respectively)."
NCBI gene IDs:
"Fstl1: 14314
S100a4: 20198"
Figure legend: "Genotypes are indicated as WT for S100a4cre-/- x Fstl1flox+/flox+ mice and cfKO for S100a4cre+/- x Fstl1flox+/flox+. (B) qPCR analysis of mRNA expression of Fstl1 and Tgfβ1 in sham and post-MI heart. Error bars represent mean ± SEM (n=16 and 15 for WT and cfKO sham group, n=15 and 14 for WT and cfKO MI group, respectively). Statistical analysis was performed by two-way ANOVA. Post Hoc test was performed by Tukey test."
NCBI gene IDs:
"Fstl1: 14314
S100a4: 20198
Tgfβ1: 21803"
Figure legend: "Genotypes are indicated as WT for S100a4cre-/- x Fstl1flox+/flox+ mice and cfKO for S100a4cre+/- x Fstl1flox+/flox+. (C) Western blot analysis of Fstl1 protein expression in ischemic and sham-operated hearts at day 7 after the surgery. Quantified values of Fstl1 protein in WT and cfKO mouse hearts normalized by GAPDH band intensity are shown. Statistical analysis was performed by two-way ANOVA. Post Hoc test was performed by Tukey test. Error bars represent mean ± SEM (n=5 for each sham group and n=6 for each MI group)."
NCBI gene IDs:
"Fstl1: 14314
S100a4: 20198"
Figure legend: "Genotypes are indicated as WT for S100a4cre-/- x Fstl1flox+/flox+ mice and cfKO for S100a4cre+/- x Fstl1flox+/flox+. (D) Fstl1 protein expression in isolated cardiac fibroblasts from cfKO and littermate WT neonatal mice. Cell lysate and its media from cells cultured for 24 hours without FBS were assessed by western blotting. Error bars represent mean ± SEM (n=3 per group). Statistical analysis was performed by unpaired t-test (2-tailed). The experiments were performed twice independently."
NCBI gene IDs:
"Fstl1: 14314
S100a4: 20198"
Figure legend: "(A) Mouse survival curve after MI and sham surgery. The mortality of the WT and Fstl1-cfKO mice after MI surgery was 27.1% and 46.7%, respectively. Log-rank (Mantel-Cox) test was used for statistical analysis (n=16 for WT sham, n=15 for Fstl1-cfKO sham, n=59 for WT MI and n=30 for Fstl1-cfKO MI)."
NCBI gene IDs:
"Fstl1: 14314"
Figure legend: "(A) Transcript mRNA expression of Col1a1 and Fn1 in sham and post-MI hearts. Hearts were obtained at day 7 after surgery. Error bars represent mean ± SEM (n=13-16 for each group). Statistical analysis was performed by two-way ANOVA. Post Hoc test was performed by Tukey test."
NCBI gene IDs:
"Col1a1: 12842
Fn1: 14268"