EMBO/SourceData huggingface dataset:
Figure legend: "Chk1fl/fl Hoxb8-FL progenitor cells were transduced with a retroviral construct carrying Cre and GFP (pMIG-CRE-IRES-GFP). Cells were FACS-sorted 72h after transduction with the retroviral construct and processed for western blotting using the indicated antibodies."
NCBI gene IDs:
"GFP: 
Chk1: 12649
Cre: 2777477
CRE: 2777477
pMIG: 17339"
Figure legend: "Percentage of GFP+ cells indicative for CRE expression and recombination in the peripheral blood after tamoxifen administration by gavage (5 x 2 mg, 200 μl/mouse; daily). Bars represent means ± S.E.M. (Day 0 N=9/10, WT/Chk1fl/fl, Day 7 N=8/7, Day 14 N=3/3)."
NCBI gene IDs:
"Chk1: 12649
CRE: 2777477"
Figure legend: "Quantification of Chk1-del PCR-product enrichment in DNA from GFP+ vs. Tomato+ bone marrow cells sorted on day 8 after first TAM administration. Transgenic Cre levels were quantified in parallel for reference. Bars represent means ± S.E.M. from n=5 mT/mG Vav-CreERT2 Chk1fl/fl mice."
NCBI gene IDs:
"mG: 
mT: 
Chk1: 12649
Cre: 2777477
ERT2: 2099"
Figure legend: "Percentage of living LK or LSK-cells and (F) percentage of GFP+ LK or LSK-cells found in the bone marrow of mT/mG Vav-CreERT2 (WT) and mT/mG Vav-CreERT2 Chk1fl/fl (Chk1fl/fl) mice on day 8 or 15 post first TAM administration. Bars in (E)/(F) represent means ± S.E.M. (Day 8 N=8/8, WT/Chk1fl/fl, Day 15 N=7/8)."
NCBI gene IDs:
"mG: 
mT: 
Chk1: 12649
Cre: 2777477
ERT2: 2099"
Figure legend: "GFP+ (CRE) and Tomato+ (CRE-) total bone marrow cells were sorted on day 8 after first TAM-administration and processed for Western Blot using the indicated antibodies. Sorted cells from three animals - mT/mG Vav-CreERT2 (WT) and mT/mG Vav-CreERT2 Chk1fl/fl (Chk1fl/fl) - were pooled per lane."
NCBI gene IDs:
"mG: 
mT: 
Chk1: 12649
CRE: 2777477
Cre: 2777477
ERT2: 2099"
Figure legend: "(A) Immunoblot analysis. Primary hepatocytes prepared from wild-type mice were infected with adenovirus expressing GFP or NBR1 under the CAG promoter for 48 hrs. Cell lysates were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
NBR1: 17966"
Figure legend: "(B) Real-time PCR. Total RNAs were prepared from primary hepatocytes described in (A). Values were normalized against the amount of mRNA in the hepatocytes expressing GFP. The experiments were performed three times. Data are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP.: "
Figure legend: "(C) Immunofluorescence microscopy. Wild type hepatocytes were infected with LacZ or NBR1 adenovirus for 48 hrs and then immunostained with anti-p62 and anti-NBR1 antibodies. Each inset is a magnified image. Bars: 20 µm. The number and size of p62-bodies were measured in more than 100 cells. Data are shown as means ± s.e. ***P &lt; 0.001 as determined by Welch's t-test."
NCBI gene IDs:
"LacZ: 
NBR1: 17966"
Figure legend: "(D) FRAP assay. Wild type hepatocytes cultured in glass-bottom plates were infected with GFP-NBR1 adenovirus for 48 hrs. The signal recovery after photobleaching was measured, quantified from at least three independent biological replicates, and is represented as means ± s.e. Bar: 1 µm."
NCBI gene IDs:
"GFP: 
NBR1: 17966"
Figure legend: "(A) Immunoblot analysis. Primary hepatocytes prepared from wild-type mice were infected with adenovirus expressing GFP-Nrf2 and GFP or NBR1 for 48 hrs. Total cell lysates were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± s.e. **P &lt; 0.01 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
NBR1: 17966
Nrf2: 18024"
Figure legend: "(B) Immunoblot analysis. Primary hepatocytes prepared from p62f/f and p62f/f; Alb-Cre mice were infected with adenovirus against GFP or NBR1 for 48 hrs. Cytosolic and nuclear fractions were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin (cytosolic proteins) or Lamin B (nuclear proteins). Data are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Alb: 11657
Cre: 2777477
NBR1: 17966
p62: 18412"
Figure legend: "(C) Immunofluorescence microscopy. Primary hepatocytes from wild type mice were infected with adenovirus expressing GFP-Nrf2 and LacZ or NBR1 for 48 hrs. More than 100 cells were assayed for the nuclear signal of Nrf2. Data are shown as means ± s.e. ***P &lt; 0.001 as determined by Welch's t-test. Bars: 10 µm."
NCBI gene IDs:
"GFP: 
LacZ: 
NBR1: 17966
Nrf2: 18024"
Figure legend: "(D) Total RNAs were prepared from primary hepatocytes described in (B). Values were normalized against the amount of mRNA in the p62f/f hepatocytes expressing GFP. The experiments were performed at least three times. Data are are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
p62: 18412"
Figure legend: "(A) Immunofluorescence microscopy. Primary hepatocytes prepared from p62f/f mice were infected with or without adenovirus Cre recombinase for 48 hrs and then additionally infected with adenovirus LacZ or NBR1. 24 hrs after the infection, the hepatocytes were immunostained with anti-Keap1, anti-p62 and anti-NBR1 antibodies. Each inset is a magnified image. Bars: 10 µm."
NCBI gene IDs:
"LacZ: 
Cre recombinase: 2777477
NBR1: 17966
p62: 18412"
Figure legend: "(C) Primary hepatocytes prepared from p62f/f; Alb-Cre mice were co-infected with adenovirus GFP or NBR1 in combination with wild-type p62 or the mutants for 48 hrs. Cell lysates were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± s.e. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Alb: 11657
Cre: 2777477
NBR1: 17966
p62: 18412"
Figure legend: "(D) RT qPCR analysis. Total RNAs were prepared from hepatocytes described in (C). Values were normalized against the amount of mRNA in the hepatocytes expressing GFP. The experiments were performed three times. Data are shown as means ± s.e. *P &lt; 0.05, and **P &lt; 0.01 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: "
Figure legend: "(A) Immunoblot analysis. Primary hepatocytes prepared from Nrf2f/f and Nrf2f/f; Alb-Cre mice were infected with adenovirus expressing GFP or NBR1 for 48 hrs. Cytosolic and nuclear fractions were prepared and subjected to immunoblot analysis with the indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin (cytosolic proteins) or Lamin B (nuclear proteins). Data are shown as means ± s.e. *P &lt; 0.05, and **P &lt; 0.01 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Alb: 11657
Cre: 2777477
NBR1: 17966
Nrf2: 18024"
Figure legend: "(B) Real-time PCR. Total RNAs were prepared from primary hepatocytes described in (A). Values were normalized against the amount of mRNA in the Nrf2f/f hepatocytes expressing GFP. The experiments were performed at least three times. Data are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Nrf2: 18024"
Figure legend: "(C) Immunofluorescence microscopy. Hepatocytes isolated from Nrf2f/f and Nrf2f/f; Alb-Cre mice were infected with LacZ or NBR1 adenovirus for 48 hrs and then immunostained with anti-p62 and anti-NBR1 antibodies. Each inset is a magnified image. Bars: 20 µm. The number and size of p62-bodies were measured in more than 100 cells. Data are shown as means ± s.e. ***P &lt; 0.001 as determined by Welch's t-test."
NCBI gene IDs:
"LacZ: 
Alb: 11657
Cre: 2777477
NBR1: 17966
Nrf2: 18024"
Figure legend: "(B) Fluorescence microscopy. p62-deficient MEFs were co-transfected with mCherry-GFP-p62 in combination with wild-type NBR1 or NBR1 D50R mutant. 48 hrs after the transfection, fluorescence images were observed. Bars: 10 µm. The number of puncta positive for only red-signal in each MEFs was counted in more than 100 cells. Bar graphs are represented as means ± s.e. ***P &lt; 0.001 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
mCherry: 
NBR1: 17966
p62: 18412"
Figure legend: "(A) Immunoblot analysis. Primary hepatocytes were isolated from wild-type (wt), p62f/f; Alb-Cre (p62KO) and Nrf2f/f; Alb-Cre (Nrf2KO) mice. The hepatocytes were challenged with 10 μM sodium arsenite [As (III)] for 12 hrs. Cell lysates were prepared and subjected to immunoblot analysis with anti-NBR1 and anti-Actin antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± s.e. *P &lt; 0.05, and **P &lt; 0.01 as determined by Welch's t-test."
NCBI gene IDs:
"Alb: 11657
Cre: 2777477
Nrf2: 4780
p62: 18412"
Figure legend: "A) Immunoblot analysis. Primary hepatocytes were isolated from Nbr1f/f mice and then infected with adenovirus GFP or Cre for 48 hrs. Thereafter, the hepatocytes were divided into 3 groups: 1. Cultured in regular medium (-), 2. Treated with 10 μM As (III) for 12 hr [As (III)] and 3. After treated with 10 μM As (III) for 12 hr, cultured in regular medium for another 12 hr (Wash). Cell lysates were prepared and subjected to immunoblot analysis with indicated antibodies. Data shown are representative of three separate experiments. Bar graphs indicate the quantitative densitometric analysis of the indicated proteins relative to actin. Data are shown as means ± s.e. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Cre: 2777477
Nbr1: 17966"
Figure legend: "B) Real-time PCR. Total RNAs were prepared from primary hepatocytes described in (A). Values were normalized against the amount of mRNA in the Nbr1f/f hepatocytes expressing GFP under normal culture conditions. The experiments were performed at least three times. Data are shown as means ± s.e. *P &lt; 0.05 as determined by Welch's t-test."
NCBI gene IDs:
"GFP: 
Nbr1: 17966"
Figure legend: "(C) Immunofluorescence microscopy. Nbr1f/f hepatocytes were infected with LacZ or Cre adenovirus for 48 hrs and, when indicated, challenged with 10 μM As (III) for 12 hrs. The cells were then immunostained with anti-p62 and anti-NBR1 antibodies. Each inset is a magnified image. Bars: 20 µm. The number and size of p62-bodies were measured in more than 100 cells. Data are shown as means ± s.e. **P &lt; 0.01, and ***P &lt; 0.001 as determined by Welch's t-test. (D) Role of NBR1 in the p62-liquid droplets and the p62-mediated Nrf2 activation. Oxidative stress up-regulates the level of NBR1, which enhances the phosphorylation and liquid-droplet formation of p62. The resulting p62-droplets are resistant to autophagic degradation and serve as signaling nodes for the activation of Nrf2. Since p62 is one of the targets of Nrf2, increased level of p62 in cooperation with NBR1 promote the formation of liquid droplets and contributes to the persistent activation of Nrf2 against oxidative stress. "
NCBI gene IDs:
"LacZ: 
Cre: 2777477
Nbr1: 17966"
Figure legend: "A) Family pedigree with SEC16B homozygosity in the patient and heterozygosity in the mother and one brother. B) Validation of the SEC16B mutation by Sanger sequencing. Sanger sequencing of gDNA of the patient, brother, mother and healthy control. Magnification of the electropherogram shows the position c.424 and the base change CT."
NCBI gene IDs:
"SEC16B: 89866"
Figure legend: "C-F) C) COL1A1 and COL1A2 expression in the ER of SEC16B patient and control fibroblasts assessed by western blotting. KDEL, Calnexin, BIP and PDI, all ER-resident proteins were used as ER markers. E) Golgi complex was isolated according to the method described in Current Protocols in Cell Biology, Unit 3.9 from healthy control cells and patient derived fibroblasts. Subsequently, COL1A1 and COL1A2 expression in the Golgi complex of SEC16B patient and control fibroblasts were determined by western blotting. GM130 (Golgi-resident protein) was used as marker for Golgi complex. All cells were treated with ascorbic acid 0.05 mg/ml for 20 hours before the Golgi complex was isolated. D, F) Quantification of the bands that was done with Bio-Rad Image Lab 6.1 software to calculate the expression levels. COL1A1 and COL1A2 band intensities were either normalized to (D) calnexin or (F) GM130. The normalized intensities were reported as fold change patient to control cells. Columns represent the mean of three (D) or two (F) independent experiments (raw values: 0.27 and 0.53 for COL1A1 and 0.29, 0.21 for COL1A2) respectively. Biological replicates were performed; bars, standard error of the mean (SEM); *significant (p &lt; 0.05) differences obtained by using unpaired two-tailed t test"
NCBI gene IDs:
"SEC16B: 89866"
Figure legend: "A) Micrograph of thin-section electron microscopy for fibroblasts from SEC16B mutant patient cells and healthy control cells. Arrows point to rER, arrowheads to MVBs/MLBs, star indicates autophagosome. Scale bar is 1 µm. Summary of the number of the autophagosomes."
NCBI gene IDs:
"SEC16B: 89866"
Figure legend: "Patient cells were either transfected with pCDNA3 empty vector (empty vector) or pcDNA3 which encodes wt SEC16B (TF). Cells were then selected with puromycin and single colonies were picked up and expanded. Established single colonies were used. B) Immunofluorescence labeling of the control and the patient fibroblasts for COL1A1 marker proα1 (I) and rough ER marker KDEL. The staining showed that while COL1A1 is accumulated in the ER of patient cells transfected with empty vector, it is not accumulated in the ER of the patient cells transfected with SEC16B wt. Scale bar is 25 µm"
NCBI gene IDs:
"SEC16B: 89866"
Figure legend: "Patient cells were either transfected with pCDNA3 empty vector (empty vector) or pcDNA3 which encodes wt SEC16B (TF). Cells were then selected with puromycin and single colonies were picked up and expanded. Established single colonies were used. D) LC3B showed reduced expression in the TF SEC16B mutant cells (similar to expression in healthy control Fig. 5B) when compared to empty vector transfected patient cells. E) Quantification of the bands. All band intensities were normalized to those of Actin and reported as fold change patient to control cells. Columns represent the mean of three independent experiments, biological replicates were performed; bars, standard error of the mean (SEM); **highly significant (p &lt; 0.001 differences obtained by using unpaired two tailed t-test."
NCBI gene IDs:
"SEC16B: 89866"
Figure legend: "A) HeLa cells were transfected with plasmids encoding GFP-tagged SEC16B-R142W. After 24 hours, cells were fixed and immunostained against SEC13 to label ERES. Green color shows SEC16B-R142W expression, red colors shows SEC13 expression and yellow color shows the overlap of the two colors. The scale bars are 10 µm."
NCBI gene IDs:
"GFP: 
SEC16B: 89866"
Figure legend: "D) HepG2 cells were transfected with the indicated siRNA. After 48h, cells were transfected with plasmids encoding signal sequence GFP (ssGFP) as part of a RUSH construct. After 24 hours, cells were treated with 40 mM biotin for 15 minutes followed by fixation and immunostaining for GM130 to label the Golgi complex. The scale bars are 10 µm."
NCBI gene IDs:
"signal sequence GFP: 
ssGFP: "
Figure legend: "A: Immunoblot analysis of wild type (WT), FAM83D-/- knockout (KO) and FAM83DGFP/GFP knockin (KI) U2OS cell lines. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"GFP: 
FAM83D: 81610"
Figure legend: "A: STLC-synchronised mitotic (M) wild-type (WT), FAM83D-/- knockout (KO) and FAM83DGFP/GFP knockin (KI) U2OS cells were subjected to anti-CK1α immunofluorescence and GFP fluorescence microscopy. DNA is stained with DAPI. Scale bars, 20 µm."
NCBI gene IDs:
"GFP: 
FAM83D: 81610"
Figure legend: "B: STLC-synchronised mitotic (M) FAM83D-/- knockout (KO), FAM83DGFP/GFP knockin (KI) and FAM83DGFP/GFP(F283A) knockin (FA) U2OS cells were subjected to anti-CK1α immunofluorescence and GFP fluorescence microscopy. DNA is stained with DAPI. Scale bars, 20 µ"
NCBI gene IDs:
"GFP: 
FAM83D: 81610"
Figure legend: "F: KI cells were infected with retroviruses encoding either VHL, aGFP.16, or VHL-aGFP.16. Uninfected cells were used as a control. Cells were lysed and subjected to IB with the indicated antibodies. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"GFP: 
VHL: 7428"
Figure legend: "B: FAM83D-/- (KO), wild-type (WT), and two independent clones from a CRISPR/Cas9-mediated knockin rescue of FAM83D cDNA into FAM83D-/- cells (c. 6 and c. 11), were synchronized in mitosis (M) with STLC. Asynchronous (AS) cells were included as a control. Cells were lysed and subjected to immunoblotting (IB) with the indicated antibodies. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"CRISPR: 
FAM83D: 81610
Cas9: 901176"
Figure legend: "G: Asynchronous (AS) FAM83DGFP/GFP/ mCherry/mCherryCSNK1A1 knockin U2OS cells were fixed and imaged. Representative images from the indicated cell cycle stages are included. Scale bars, 10 µm."
NCBI gene IDs:
"GFP: 
mCherry: 
CSNK1A1: 1452
FAM83D: 81610"
Figure legend: "A: Nocodazole-synchronised mitotic FAM83DGFP/GFP knockin U2OS cells were lysed and subjected to GFP TRAP immunoprecipitation (IP), followed by incubation ± λ-phosphatase. Asynchronous (AS) cells were used as a control. Whole cell extracts (input) and IP samples were subjected to SDS-PAGE and subsequent immunoblotting (IB) with the indicated antibodies. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"GFP: 
FAM83D: 81610"
Figure legend: "F: STLC-synchronised mitotic wild-type U2OS cells were subjected to qRT-PCR analysis using primers for FAM83D, HMMR, CSNK1A1, and CCNB1. AS cells were used as a control. Error bars, SEM; *, P&lt;0.01; Student's T-test. n=3"
NCBI gene IDs:
"CCNB1: 891
CSNK1A1: 1452
FAM83D: 81610
HMMR: 3161"
Figure legend: "H: STLC-synchronised mitotic wild-type (WT) and HMMR knockout (KO) mouse embryonic fibroblasts (MEFs) were lysed and subjected to IB with the indicated antibodies. AS cells were included as a control. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"HMMR: 15366"
Figure legend: "B: STLC-synchronised mitotic FAM83DGFP/GFP knockin (KI), FAM83DGFP/GFP (F283A) (FA), and FAM83DGFP/GFP(F283A) stably expressing aGFP.16-CK1α (FA + aGFP.16-CK1α) U2OS cells were subjected to GFP TRAP immunoprecipitation (IP), followed by immunoblotting (IB) with the indicated antibodies. Asynchronous (AS) cells were used as controls. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"GFP: 
CK1α: 1452
FAM83D: 81610"
Figure legend: "C: FA cells were infected with retroviruses encoding wild-type aGFP.16-CK1α (WT), or aGFP.16-CK1α with one of two distinct CK1α kinase-inactive mutants (D136N or K46D). Uninfected cells (-) were included as a control. Cells were lysed, subjected to anti-GFP IP and IB with the indicated antibodies. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"GFP: 
CK1α: 1452"
Figure legend: "F: FAM83D-/- knockout (KO), KI, and FA cells were synchronised in mitosis (M) using STLC. Lysed extracts were subjected to anti-GFP IPs, followed by ATP [γ-32P] kinase assays, using an optimised CK1 substrate peptide (CK1tide). AS cells were used as controls. Error bars, SEM; P&lt;0.01; ANOVA. n=3. Input and IP samples were analysed by IB with the indicated antibodies. Data information: All blots are representative of at least 3 independent experiments."
NCBI gene IDs:
"FAM83D: 81610"
Figure legend: "B. Carfilzomib, Bortezomib and MLN9708 activate  luciferase activity in   M2  macrophages  derived from  Il-1β-luciferase transgenic  mice. BMDMs from  IL-1β-luciferase transgenic mice were treated by  IL-4  (20 ng/mL) for 24 hours to differentiate into mature M2  macrophages, and then used for screening of FDA compounds (5 μM). The X axis stands for the code of the drug."
NCBI gene IDs:
"luciferase: 
Il-1β: 16176
IL-1β: 16176"
Figure legend: "F, G. Carfilzomib, Bortezomib  and  MLN9708 promote the mRNA  expression of  Il-1β in M2 macrophages. BMDMs (F) or Raw264.7 cells (G) were pretreated by  IL-4 (20 ng/mL) for 24 hours, then treated by DMSO, Carfilzomib (1 μM), Bortezomib (1 μM), or MLN9708 (2 μM). RNA was extracted from  BMDMs or Raw264.7 cells 6 hours after stimulation and the expression  of Il-1β were quantified through  RT-qPCR. Data from three experiments are presented as the mean ± SD. T test was used for statistical analysis of differences between groups. ***p &lt; 0.001 (student's t-test). H, I. Carfilzomib, Bortezomib and MLN9708 promote the secretion of inflammatory cytokine  IL-1β  in  M2 macrophages. BMDMs  (H) or Raw264.7 cells (I) were pretreated by IL-4 (20 ng/mL) for 24 hours, then stimulated by DMSO, Carfilzomib (500 nM), Bortezomib (500 nM) or MLN9708 (500 nM). Secretion of IL-1β were measured through  ELISA 24 hours later. Data from three experiments are presented as the mean ± SD. T test was used for statistical analysis of differences between groups. **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Il-1β: 16176"
Figure legend: "A. The most effective one among the candidate compounds,  Carfilzomib, significantly promotes the  expression of M1 macrophage markers, as well as reduces the expression of M2 macrophage  markers. BMDMs were pretreated by IL-4  (20 ng/mL) for 24 hours, then stimulated by DMSO or Carfilzomib (1 μM). RNA was extracted from cells 6 hours after stimulation and the  expression of M1 (Il-6/Inos) or  M2 (Cd206/Arg1)  macrophage markers were quantified through  RT-qPCR. Data from three experiments are presented as the mean ± SD. T test was used for statistical analysis of differences between groups. ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Arg1: 11846
Il-6: 16193
Inos: 106736473
Cd206: 17533"
Figure legend: "E. Carfilzomib promotes the  phagocytosis  of macrophages. BMDMs  were pretreated by IL-4 (20 ng/mL) for 24 hours, then stimulated by  DMSO or Carfilzomib (500 nM) for 12 hours. After starving for 2 hours and  stained  with Red membrane dye, BMDMs (red) were incubated with  L1210-GFP cells (green) in serum-free medium for another 2 hours. Phagocytosis effect was observed and photographed under fluorescence microscope. Scale bars, 50 μm (up), 20 μm (down). The yellow arrows indicated L1210 that is phagocytosed by macrophages. F. Statistics represent the  number of phagocytosis L1210 in 100  macrophages. Data are means ± SD of three independent experiments. **p &lt; 0.01 (student's t-test)."
NCBI gene IDs:
"GFP: "
Figure legend: "M. Carfilzomib  promotes the M1-polarization of Tumor associated macrophages  in vitro. BMDMs  were cultured with tumor culture supernatant (TSN) produced by L1210 cells, followed by activation with DMSO or Carfilzomib  (1 μM). RNA was extracted from cells and the expression of  Il-1β, Il-6, Inos were quantified through  RT-qPCR  6 hours after stimulation. The data are means ± SD of three independent experiments. **p &lt; 0.01, ***p &lt; 0.001 (student's t-test)."
NCBI gene IDs:
"Il-1β: 16176
Il-6: 16193
Inos: 106736473"