EMBO/SourceData huggingface dataset:
Figure legend: "D and E) Confocal fluorescent images obtained after transient expression of MplmOrange2 and immuno‐fluorescent labeling of endogenous JAK2 in K562 (D) or HEL (E) cells. Profiles of fluorescence (across individual cells and indicated by a white arrow) are showing a good colocalization of Mpl and JAK2WT in K562 cells at the plasma membrane (black arrow heads) but no colocalization have been observed between MplmOrange2 and JAK2V617F in HEL."
NCBI gene IDs:
"JAK2: 3717
Mpl: 4352"
Figure legend: "F and G) Confocal fluorescent images obtained after transient coexpression of MplmOrange2 and either JAK2WTmCitrine (F) or JAK2V617FmCitrine (G) in K562 cells. Profiles of fluorescence (white arrows) are showing a very good colocalization of MplmOrange2 with JAK2WTmCitrine, especially at the plasma membrane (black arrow heads), but a very poor colocalization with JAK2V617FmCitrine (grey arrow heads). Nuclei are labeled with Hoechst and scale bars = 5 µm. R: Pearson's correlation coefficient."
NCBI gene IDs:
"JAK2: 3717
Mpl: 4352"
Figure legend: "HEL (A) and K562 (B) cells treated with non‐targeting (NT) or anti‐JAK2 siRNA analyzed by western blot."
NCBI gene IDs:
"JAK2: 3717"
Figure legend: "C) Confocal live cell (upper panel), or fixed cells (lower left panel), imaging of K562 cells expressing MpleGFP or MplmKO2 treated with NT or anti‐JAK2 siRNA showing a difference of distribution of intracellular Mpl in the siRNA treated cells compare to the control cells. After treatment with anti‐JAK2 siRNA, Mpl strongly colocalizes with the ER marker, calnexin. This colocalization in treated vs non‐treated cells is quantified and shown in Figure C lower right panel. Nuclei are labeled with Hoechst and scale bars = 5 µm."
NCBI gene IDs:
"JAK2: 3717
Mpl: 4352"
Figure legend: "Intracellular Mpl pools partially colocalize with ER markers and colocalize with autophagic and lysosomal markers in K562 and HEL cells. A-M) Confocal fluorescent images obtained after transient expression of MplmOrange2 or MplmKO2 and indicated labeling in K562 (A-F) or HEL (G-M) cells. Plots at right of each image show fluorescence intensities for each emission spectrum at points along the line drawn across each image. Black arrows in the plots point to overlap between MplmKO2 and either ER‐tracker (C,I), or Sec31a (B,J) that marks ER exit sites. MplmOrange2 did not colocalize with markers for the Golgi (Golgin‐97) or early endosomes (EEA1) in either cell line. However, MplmKO2 colocalized greatly with autophagic (LC3) (E,L) and lysosomal (LAMP1) (F,M) markers. Nuclei are labeled with Hoechst and scale bars = 5 µm. R: Pearson's correlation coefficient."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "Intracellular Mpl pools partially colocalize with ER markers and colocalize with autophagic and lysosomal markers in K562 and HEL cells. A-M) Confocal fluorescent images obtained after transient expression of MplmOrange2 or MplmKO2 and indicated labeling in K562 (A-F) or HEL (G-M) cells. Plots at right of each image show fluorescence intensities for each emission spectrum at points along the line drawn across each image. Black arrows in the plots point to overlap between MplmKO2 and either ER‐tracker (C,I), or Sec31a (B,J) that marks ER exit sites. MplmOrange2 did not colocalize with markers for the Golgi (Golgin‐97) or early endosomes (EEA1) in either cell line. However, MplmKO2 colocalized greatly with autophagic (LC3) (E,L) and lysosomal (LAMP1) (F,M) markers. Nuclei are labeled with Hoechst and scale bars = 5 µm. R: Pearson's correlation coefficient."
NCBI gene IDs:
"Mpl: 4352///4352"
Figure legend: "Confocal fluorescent images obtained after transient expression of Mpl tagged with mKO2 and miniSOG in K562 (A) and HEL (B) cells. Electron micrographs of K562 (C) and HEL (D) cells transiently expressing MplmKO2‐miniSOG. Dark precipitate indicates the presence of the miniSOG‐tagged Mpl protein after photo‐precipitation of DAB. White boxes indicate original location of higher magnification images with corresponding letters."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "H) High magnification electron micrograph of HEL cells treated in the same conditions as Figure D, cells transiently expressing MplmKO2-miniSOG , showing different stage of formation of APs (black arrows numbered from stages 1-4). Scale bars = 5 µm (A-D) or 100 nm (E-H)."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "E) Western blot analysis of protein phosphorylation in HEL, K562Mpl‐mKO2 and K562Mpl‐mOrange2 cells after Tpo stimulation (50 ng/mL Tpo, 10 min)."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "A and B) Surface biotinylation and total lysates western blot analysis of HEL or K562Mpl‐mKO2 cells after treatments for 60 min with Tpo, ionomycin (I), PMA, Mon or A23187."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "C) Total lysate, surface biotinylation and exosomal fraction western blot analysis of HEL and K562Mpl‐mKO2 cells treated with Mon or A23187 for 15 h."
NCBI gene IDs:
"Mpl: 4352"
Figure legend: "F) Total lysate and surface biotinylation western blot analysis of HEL cells overexpressing ATG5 K130R‐V5 6 h post‐transfection."
NCBI gene IDs:
"ATG5: 9474"
Figure legend: "G) Total lysate and surface biotinylation western blot analysis of HEL cells overexpressing either GRASP55-V5 or HA-STX5."
NCBI gene IDs:
"GRASP55: 26003
STX5: 6811"
Figure legend: "C - Whole cell lysates were prepared with TG2+/+ and TG2-/- MEFs infected or not for 48 h with C. trachomatis L2 in the presence or not of BP, and analyzed as in (A)."
NCBI gene IDs:
"TG2: 21817"
Figure legend: "E - Cells were infected with C. trachomatis L2 (MOI=1) for 24 or 46 h. Where indicated, 40 μM CP4d was added 2 hpi. tgm2 transcripts were measured by real-time RT-PCR and normalized to actin transcripts following the ∆∆Ct method. The data are presented as relative mRNA levels compared to uninfected cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated at least four times. P-values of Student's ratio-paired t-test &lt;0.05 are shown."
NCBI gene IDs:
"actin: 
tgm2: 21817"
Figure legend: "F - Cells were treated for 18 h with the indicated concentration of human recombinant IL-6 before measuring TG2 transcription relative to actin like in (E). The data are presented as relative mRNA levels compared to untreated cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated at least three times. P-values of Student's ratio-paired t-test &lt;0.05 are shown."
NCBI gene IDs:
"actin: 
TG2: 21817"
Figure legend: "Cells were left uninfected or infected with C. trachomatis L2 in the presence of the indicated concentration of anti-IL-6 receptor antibodies. Forty-eight h later TG2 transcription relative to actin was measured like in (E). The data are presented as relative mRNA levels compared to uninfected/untreated cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated at least three times. P-values of Student's ratio-paired t-test &lt;0.05 are shown."
NCBI gene IDs:
"actin: 
TG2: 21817"
Figure legend: "A - HeLa cells were pre-treated with the indicated concentrations of CP4d (or DMSO alone) for 2 h before being infected with L2incDGFP at MOI=0.15. Thirty hours later the cells were disrupted and bacterial titers (IFU=inclusion forming unit) were determined by re-infecting fresh HeLa cells as described in the methods. The mean ± SD of three independent experiments are shown. P-values of Student's paired t-test are indicated when &lt;0.05."
NCBI gene IDs:
"incD: 45091107"
Figure legend: "B - HeLa cells were transfected with control siRNA or two siRNAs against TG2. Two days later, the efficiency of the silencing was assessed by western blot using anti-TG2 antibodies and anti-actin antibodies as loading control (bottom). Duplicate wells were infected with L2incDGFP and progeny was analyzed as in (A) (top). The mean ± SD of four independent experiments are shown. P-values of Student's paired t-test are indicated when &lt;0.05."
NCBI gene IDs:
"incD: 45091107
TG2: 7052"
Figure legend: "A - MEFs were grown for 24 h culture medium complemented with the indicated concentration of glucose before being infected with L2 IncDGFP bacteria (MOI = 0.2). Cells were disrupted 30 h later and the bacterial titer determined by re-infecting fresh wild type cells. The mean ± SD of three independent experiments are shown."
NCBI gene IDs:
"GFP: 
IncD: 45091107"
Figure legend: "B - Cells were infected with C. trachomatis L2 (MOI=1) for 24 or 48 h. Where indicated 40 μM CP4d was added 2 hpi. GLUT-1 and GLUT-3 transcripts were measured by real-time RT-PCR and normalized to actin transcripts following the ∆∆Ct method. The data are presented as relative mRNA levels compared to uninfected cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated four times. P-values of Student's ratio-paired t-test are indicated when &lt;0.05."
NCBI gene IDs:
"actin: 
GLUT-1: 6513
GLUT-3: 6515"
Figure legend: "C -Relationship between TGM2 and GLUT-1 (top) and GLUT-3 (bottom) expression across 265 HGSOCs. Expression comparisons were performed using Spearman's rank correlation test."
NCBI gene IDs:
"GLUT-1: 6513
GLUT-3: 6515
TGM2: 7052"
Figure legend: "D -HeLa cells were infected with C. trachomatis L2 (MOI=1) for 24 or 46 h. HIF-1α transcripts were measured by real-time RT-PCR and normalized to actin transcripts. The data are presented as relative mRNA levels compared to uninfected cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated three times. P-values of Student's ratio-paired t-test are &gt; 0.05."
NCBI gene IDs:
"actin: 
HIF-1α: 3091"
Figure legend: "E - HeLa cells were transfected with control siRNA or two siRNAs against HIF-1α. Two days later, cells were infected with C. trachomatis L2 (MOI=1) for 48 h. The indicated transcripts were measured by real-time RT-PCR and normalized to actin transcripts. The data are presented as relative mRNA levels compared to uninfected cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated three times. P-values of Student's ratio-paired t-test are indicated when &lt;0.05."
NCBI gene IDs:
"actin: 
HIF-1α: 3091"
Figure legend: "F - TG2-/- MEFs stably transformed or not with the indicated TG2 construct were infected for two days with C. trachomatis L2 (MOI=1). Mouse GLUT-1 transcripts were measured by real-time RT-PCR and normalized to mouse actin transcripts. The data are presented as relative mRNA levels compared to uninfected cells and shown as the mean ± SD. Each experiment was performed in duplicate and repeated three times. P-values of Student's ratio-paired t-test are indicated when &lt;0.05."
NCBI gene IDs:
"actin: 
GLUT-1: 20525
TG2: 21817"
Figure legend: "C - The same experimental procedure as described in (B) was applied to HeLa cells treated for 48 h prior to ionomycin treatment (8 µM) with siRNA control or directed against TG2."
NCBI gene IDs:
"TG2: 7052"
Figure legend: "C - HeLa cells treated for 72 h with siRNA targeting GFPT1 or GFPT2 were lysed. After separation with SDS-PAGE, proteins were transferred to a membrane, probed with anti-GFPT and anti-actin antibody before revelation with HRP-conjugated secondary antibodies."
NCBI gene IDs:
"GFPT1: 2673
GFPT2: 9945"
Figure legend: "D - HeLa cells were transfected with control siRNA or siRNAs against GFPT or TG2. Two days later the cells were infected for the indicated times (MOI=0.3) before fixation, rupture of the cells and measurement of bacterial diameter. The mean diameter ± SD and p-values of Student's paired t-test on 4 independent experiments are shown."
NCBI gene IDs:
"GFPT: 2673
TG2: 7052"
Figure legend: "E - HeLa cells treated for 48 h with siRNA targeting GFPT1 or not (siCTRL) were infected with C. trachomatis (MOI = 0.15). Thirty hours later cells were fixed and analyzed by flow cytometry. The percentage of infected cells (top) and the mean fluorescence of the infected population (middle) ) ± SD are shown for at least four independent experiments. Duplicate wells were lysed and used to re-infect fresh HeLa cells to determine the bacterial titer (bottom). P-values of Student's ratio-paired t-test are indicated."
NCBI gene IDs:
"GFPT1: 2673"
Figure legend: "(C) All eight subunits of the CCT chaperonin complex were enriched in U2OS-A3A  samples. Proteins detected by MS are displayed by fold enrichment (log2) from U2OS-A3A cells compared to parental cells (x-axis), and by protein abundance within the  U2OS-A3A  samples (y-axis). Significantly enriched proteins (p&lt;0.05 by one-tailed t-test, n=3) are denoted in dark blue. Components of the CCT complex  are highlighted and labeled."
NCBI gene IDs:
"A3A: 200315"
Figure legend: "(A) A3A co-precipitates  with CCT complex subunits. U2OS  and HepaRG cells with  dox-inducible A3A-HA  transgenes were treated with  dox prior to immunoprecipitation of HA. HA immunoprecipitates were analyzed by immunoblot with antibodies for  HA  and subunits of the CCT complex."
NCBI gene IDs:
"HA: 
A3A: 200315"
Figure legend: "(C) A3A interacts with CCT in  leukemia  cells. HA immunoprecipitation in  hematopoietic cell lines with inducible  A3A-HA  was analyzed by immunoblot. HA, CCT1, and CCT5  antibodies were used to evaluate co-precipitation of CCT subunits with  A3A."
NCBI gene IDs:
"HA: 
A3A: 200315"
Figure legend: "(A) CCT  co-precipitates with A3A but not A3B or  A3G. 293T cells were transfected with empty vector (V), A3A-HA, A3B-HA, or A3G-HA. CCT IP was performed from transfected cells and evaluated by immunoblot analysis using antibodies for CCT5 and HA."
NCBI gene IDs:
"HA: 
A3A: 200315
A3B: 9582
A3G: 60489"
Figure legend: "(B) A3A, but not other  APOBEC3 enzymes,  interacts with CCT complex subunits. After transfection of 293T cells with HA-tagged APOBEC3 expression plasmids, IP was performed with anti-HA  antibody and analyzed by  immunoblot  using antibodies to HA and CCT subunits. Arrows mark location of each APOBEC3  protein on HA immunoblot."
NCBI gene IDs:
"HA: "
Figure legend: "(A) Knockdown of one CCT complex member results in depletion of other CCT subunits. Following siCCT4 or siCCT7  transfection, cell lysates were evaluated by immunoblot for expression of additional CCT subunits by endogenous antibodies for CCT1, CCT4, CCT5, and CCT7. GAPDH  was used as a loading control."
NCBI gene IDs:
"CCT4: 10575
CCT7: 10574"
Figure legend: "(B) Decreased cell viability  upon CCT depletion and A3A expression. CCT4 (left) and CCT7 (right) subunits were depleted by siRNA transfection in U2OS  cells with dox-inducible  A3A transgenes. Immunoblot  analysis of cell lysates showed decreased expression of targeted CCT subunit, but no alteration in  A3A expression. Scr indicates non-targeted siRNA, used as control. Ku-86 is a loading control. Following siRNA transfection to deplete CCT subunits, U2OS-A3A  cells were treated with  dox. Viability  was determined by  colorimetric change after addition of a water-soluble tetrazolium salt. Percent viability  was normalized to untreated controls. Statistical analysis was performed using a paired two-tailed t-test, n=3 biological replicates; error bars, SEM."
NCBI gene IDs:
"A3A: 200315
CCT4: 10575
CCT7: 10574"
Figure legend: "(D) Viability of cells with catalytically inactive A3A. U2OS-A3A-C106S cells were induced with dox to express the catalytically inactive A3A mutant (C106S), depleted of CCT4  by siRNA, or combination. Cells were subsequently evaluated by live-dead staining assessed by FACS. Bar chart shows quantitation of FACS  results averaged over three biological replicates. Statistical analysis was performed using a paired two-tailed t-test, n=3; error bars, SEM. Immunoblot analysis of cell lysates showed decreased expression of CCT4 after siRNA targeting. Ku-86 is a loading control."
NCBI gene IDs:
"A3A: 200315
CCT4: 10575"
Figure legend: "(E) Increased DNA damage signaling upon CCT depletion and A3A expression. Depletion of CCT7 in K562-A3A  cells was achieved by siRNA targeting. Expression of A3A was induced by dox treatment. Intracellular  staining with anti-γH2AX  antibody was analyzed by flow cytometry. Bar chart shows quantification of FACS results obtained over three biological replicates. Statistical analysis was performed using a two-tailed t-test, n=3; error bars, SEM. Immunoblot  analysis of cell lysates showed decreased expression of CCT7  after siRNA targeting and A3A expression upon dox  treatment. Ku-86 is a loading control. **p-value &lt;0.01, ***p-value &lt;0.01, ****p-value &lt;0.0001, n.s. non-significant."
NCBI gene IDs:
"A3A: 200315
CCT7: 10574"
Figure legend: "(C) Quantification of APOBEC3  mutational signatures among three tumor types that have previously been associated with high APOBEC3 activity. Breast, bladder, and cervical cancer samples from the TCGA database were divided into CCT-mutated and non-mutated genomes, as above. The number of mutations contributing to SBS 2 and SBS 13 in each tumor type was quantified and displayed as an average. Comparison by Wilcoxon rank sum test yielded p-values denoted."
NCBI gene IDs:
"APOBEC3: 9582///200315"
Figure legend: "A. Vps10yEGFP localization. Yeast cells carrying Vps10yEGFP and expressing the indicated vps35 alleles as the sole source of Vps35 were logarithmically grown in SC medium. Their vacuoles were labelled with FM4-64. Cells were harvested by brief centrifugation and immediately imaged by confocal microscopy. Single confocal planes are shown. A brightfield image was used to outline the cell boundaries (shown in the merged images). B. Co-localization of Vps10yEGFP and FM4-64 in cells from a was measured using Pearson's coefficient. 15 Confocal planes with 20 to 30 cells each from 3 independent experiments were analyzed. Means"
NCBI gene IDs:
"vps35: 853287
Vps35: 853287"
Figure legend: "B. Influence of VPS35 variants on GLUT1. HK2 cells silenced for VPS35 were transfected with a plasmid carrying siRNA resistant wildtype or mutant forms of GFP-VPS35. GLUT1 was detected by fixation and immunofluorescence staining as in a. Maximum projections of image stacks (step size in z of 300 nm) are shown."
NCBI gene IDs:
"GFP: 
VPS35: 55737"
Figure legend: "C. Quantification of GLUT1 immunofluorescence in cells from B. Regions of interest (ROIs) corresponding to cells expressing the indicated VPS35 variants, and some regions outside the cells (background), were manually defined using ImageJ software. Total cell fluorescence was integrated and corrected for background fluorescence. 105 cells per condition stemming from 3 independent experiments were analyzed. The analysis was performed with 99% confidence: *** p &lt; 0.001."
NCBI gene IDs:
"VPS35: 55737"
Figure legend: "D. Expression of Vps35 variants in cells from B was analyzed by SDS-PAGE and Western blot against Vps35. Tubulin served as loading control."
NCBI gene IDs:
"Vps35: 55737"
Figure legend: "(B) Bar graph summarizing the efficiency of single RNAi knockdowns for 28 out of the 30 TRs, and their effects in the reprogramming efficiency in comparison to control cells expressing scramble shRNA. The efficiency of each TR's knockdown (KD) does not correlate with its effect in reprogramming efficiency. The knockdown of 9 (Class III) out of the 28 TRs decreased the efficiency of the reprogramming in a statistically significant manner (unpaired two-tailed Student's t-test, p-value &lt;0.05). Data are shown as mean ± SEM of at least two independent experiments. Class I corresponds to one TR inhibiting reprogramming, whereas Class II corresponds to 18 TRs having a weak or no effect in repogramming. Shown are the effects of 28 out of 30 TRs, because we did not succeed in knocking down Cphx and Phox2a."
NCBI gene IDs:
"Cphx: 105594
Phox2a: 11859"
Figure legend: "(E) Heatmap summarizing the most striking effects of double nine TRs KDs on the expression of the other TRs and on the expression of the Epcam and Lin28a pluripotency markers; the white color indicates no change in expression levels. The genes examined have been ranked in the heatmap according to their order of upregulation during reprogramming; Lin28a is the latest upregulated gene."
NCBI gene IDs:
"Epcam: 17075
Lin28a: 83557"
Figure legend: "(C) Shown is a bar graph depicting the effects of rescue experiments in reprogramming efficiency. MEFs undergoing reprogramming were knocked down using shRNAs for the endogenous Nanog or Irf6 expression and were co-infected with a vector expressing an shRNA-resistant human homologue of Nanog or Irf6 (hNanog OE, hIrf6 OE). Data are shown as mean ± SEM of two independent experiments."
NCBI gene IDs:
"Irf6: 54139
Irf6: 3664
Nanog: 71950
Nanog: 79923"
Figure legend: "(A) Immunostaining of CDC50A on Cdc50a knockdown neurons and controls. Accell Cdc50a siRNA pool was used to knock down Cdc50a in neurons for 7 days. CDC50A expression alongside neuronal processed was reduced after Cdc50a siRNA treatment. Scale bar, 10 µm."
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(D) IHC of Cdc50a-RNAi neurons exhibited pSIVA colocalized with synapsin I, a general synapse marker. The dotted square was zoomed-in shown on the right side. Arrows indicated where pSIVA colocalized with synapsin I. Scale bar, 5 µm."
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(D) Representative IHC images of CDC50A expression at synapses. Scale bar, 5 µm. Arrows indicate VGluT2+ RGC inputs. (E) Relative CDC50A expression at mCherry+ synapses in Cdc50a RNAi mice and controls. N = 3 for biological replicates, mean ± SD, * p &lt; 0.05 by Student's t-test. "
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(F) PSIVA staining in the dLGN. Exposed PS was detected by pSIVA on RGC inputs in Cdc50a-RNAi mice. Scale bar, 5 µm. Arrows indicate pSIVA+/mCherry+ RGC inputs. (G) Quantification of the percentage of pSIVA+/mCherry+ RGC inputs in total mCherry+ RGC inputs. N = 4 for biological replicates, mean ± SEM, **p &lt; 0.01 by Student's t-test. "
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(F) Maximum intensity z-projection images of vGluT2+ synapses in the core region of Cdc50a RNAi mice and controls. Right panel showed enlarged synapses in the boxed region. Scale bar, 20 µm."
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(D) Images of CTB488 and CTB647 labeling of RGC inputs in the dLGN. Scale bar, 20 µm. (E) Quantification of CTB488+ inputs (non-infected areas) versus CTB647+ inputs (infected areas) in Cdc50a RNAi mice and controls. N = 3 for biological replicates, mean ± SEM, **** p &lt; 0.0001, two-way ANOVA with Sidak's multiple comparisons test. "
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(A) The confocal images, 3D-rendered images and orthogonal views of microglia engulfing vGluT2+ synapses in controls and Cdc50a-RNAi mice. Scale bar, 10 µm."
NCBI gene IDs:
"Cdc50a: 69981"
Figure legend: "(A) IHC images for vGluT2 staining in the dLGN of microglial Gpr56 conditional knockout mice (Gpr56fl/fl; Cx3cr1cre/+) and controls (Gpr56+/+; Cx3cr1cre/+). Both mice were intravitreally injected with AAV2 carrying Cdc50a shRNA. The yellow squares indicated the AAV2 non-infected areas and the dotted outlines indicated the AAV2 infected areas. Scale bar, 200 µm."
NCBI gene IDs:
"Gpr56: 14766
cre: 2777477
Cx3cr1: 13051
Cdc50a: 69981"
Figure legend: "(B) Confocal images of vGluT2+ synapses in the AAV2 non-infected areas and infected areas of the dLGN. Scale bar, 20 µm. (C) Quantification of vGluT2+ synapses in the non-infected areas and infected areas of microglial GPR56 conditional knockout mice and controls. N = 4 for biological replicates, mean ± SD, * p&lt;0.05, **** p &lt; 0.0001 by two-way ANOVA with Sidak's multiple comparisons test. "
NCBI gene IDs:
"GPR56: 14766"
Figure legend: "C, Heatmap of DEGs between the Src-1/-2 dKO and WT fetuses  that are involved in the lung  development pathway (GO: 0030324)."
NCBI gene IDs:
"Src-1: 17977"
Figure legend: "A and C, mRNA levels of Arg1  and Ass1  in fetal lungs  of mice  with different genotypes at 18.5 dpc; n=8 biological replicates in each group for panel A and 7 biological replicates in each group for panel C. One-way ANOVA followed by Tukey's multiple comparison test was used to analyze the data. The data are shown as the mean ± s.e.m., *P&lt;0.05, ***P&lt;0.001, ****P&lt;0.0001, compared with the WT group."
NCBI gene IDs:
"Arg1: 11846
Ass1: 11898"