EMBO/SourceData huggingface dataset:
Figure legend: "(r,s) C57BL/6 mice were infected intranasally with the Aspergillus crf1Δ mutant (r) or the AF293 (s) strain and monitored for fungal growth (log10 colony-forming units (CFU)±s.d.) in the lungs 2 days later. Fungal cells were exposed or not (none) to 10 ng ml−1 IL-17A for 4 h, extensively washed and injected. Data are mean±s.d. (n=4). The actual P-values are indicated (two-tailed t-test)."
NCBI gene IDs:
"crf1: "
Figure legend: "(D) HeLa cells were transfected with 10 nM control (n=84) or  Nup50 siRNA (n=171), 20 nM control (n=101) or Nup50 siRNA (n=157) and 40 nM control (n=86) or  Nup50 siRNA (n=204). 72 h post transfection, cells were fixed with 4% PFA  and stained  with antibodies against Nup50 and mAB414, chromatin was labeled with  DAPI. Scale bars: 50 µm. Quantitation of the mAB414 rim intensity at three different Nup50 RNAi and control oligo concentrations. The means are indicated as diamonds, error bars show the standard deviations. P-values have been calculated from a student t-test comparing the mean between the experimental conditions."
NCBI gene IDs:
"Nup50: 10762"
Figure legend: "(A) Mouse 3T3-NIH cells were transfected with 20 nM control, Nup50A, Nup50B or a combination of Nup50A and Nup50B  siRNA. After 72h cells were fixed and stained with mAB414 (green) and antibodies against mouse Nup50 (red). Chromatin is  stained with DAPI (blue). The merge of the three channels is shown on the left column. Scale bar: 50 µm. An insert shows a zoom on a representative  nucleus for each picture. Scale bar: 10 µm."
NCBI gene IDs:
"Nup50A: 18141
Nup50B: 18141"
Figure legend: "(A, B) Demembranated sperm chromatin was preincubated in  Xenopus egg  extract, depleted for  MEL28/ELYS or Nup50 (B) or control treated (Mock, A). After 10 min,  membranes were added to the reaction. Reactions were stopped at the indicated time points  by fixation and analyzed by confocal microscopy after  immunostaining with α-MEL28/ELYS  (A), α-Nup50 (B), and mAb414 (A, B). Chromatin  was stained  with  DAPI (blue in the overlay). Scale bar: 10 μm."
NCBI gene IDs:
"ELYS: 397707
MEL28: 397707
Nup50: 503671///503675"
Figure legend: "3 µM recombinant EGFP, EGFP-tagged MEL28/ELYS (aa 2290-2408) or  EGFP-tagged  Nup50 were incubated with empty or DNA-coated magnetic beads, which were chromatinized with Xenopus egg   extracts (right panel) or unchromatinized DNA-beads (left panel). After 3h the beads were re-isolated, washed, co-stained with DAPI  and analyzed by  confocal microscopy. Scale bar: 10 µm."
NCBI gene IDs:
"EGFP: 
ELYS: 
MEL28: 397707
Nup50: 503671///503675"
Figure legend: "(C)  HeLa cells were transfected with EGFP-Nup50 comprising different point mutations in the minimal NPC binding region and analyzed as in (A). Scale Bar: 10 µm."
NCBI gene IDs:
"EGFP: 
Nup50: 18141"
Figure legend: "(D)  GST-fusion constructs of the  Xenopus Nup50 minimal NPC binding fragment (aa 144-189) comprising no or single point mutations were incubated with Xenopus egg extracts. Starting material as well as bound proteins were analyzed by Western blotting with indicated antibodies. The quantitation shows the average MEL28/ELYS and Nup153 bead bound signal from three independent experiments, normalized to the signal of their respective wild-type GST fusion Data points from individual experiments are indicated."
NCBI gene IDs:
"Nup50: 503671///503675"
Figure legend: "(A) Confocal microscopy images of  nuclei  assembled for 120 min in mock depleted, Nup50 depleted (∆Nup50), and Nup50 depleted Xenopus egg extracts supplemented with recombinant wild-type Nup50  or different mutants. Nuclei  were fixed in 4% PFA and 0.5 % glutaraldehyde, stained  for NPCs  (mAB414) and the chromatin  (DAPI). Scale bar: 10 µm. (B) Average percentage of mAB414 positive  nuclei for 100 randomly chosen chromatin substrates in each of at least three independent experiments shown in (A). Data points from the individual experiments are indicated. "
NCBI gene IDs:
"Nup50: 503671///503675"
Figure legend: "(C) Confocal microscopy  images of  nuclei  assembled with N-terminal Nup50 truncations and an minimal N-terminal fragment. Samples were analyzed as in (A). (D) Average percentage of mAB414 positive nuclei  for 100 randomly chosen  chromatin substrates in each of at least three independent experiments shown in (C). Data points from the individual experiments are indicated. "
NCBI gene IDs:
"Nup50: 503671///503675"
Figure legend: "(B)  Confocal microscopy images of fixed  nuclei  assembled for 120 min in mock depleted (mock) and Nup50 depleted (∆Nup50)  Xenopus egg  extracts supplemented with recombinant  mouse Nup50  orthologues. Nuclei  were stained for Nup50 (Nup50  antibody for Xenopus protein,  His6  for  mouse protein) and NPCs (mAB414, red) on the chromatin  (DAPI). Scale bar: 10 µm. (C) The average percentage of mAB414 positive nuclei for 100 randomly chosen  chromatin substrates in each of three independent experiments (performed as in B) is shown. Data points from individual experiments are indicated. "
NCBI gene IDs:
"Nup50: 503671///503675"
Figure legend: "HEK293T cells were transfected with empty  FLAG-tag vector as a control or FLAG-Nup50 N-terminal fragment (aa 1-120 of the human sequence). 24 h post transfection cells were lysed,  FLAG-tagged proteins immuno-isolated and analyzed by mass spectrometry with the Volcano blot showing the identified interactors of Nup50 (yellow, data from three independent experiments) Data points from individual experiments are indicated. KPNA2  is the gene name of  importin α."
NCBI gene IDs:
"FLAG: 
Nup50: 10762"
Figure legend: "HEK293T  cells were transfected with empty FLAG-tag vector as a control or FLAG-Nup50 N-terminal fragment (aa 1-120 of the human sequence). 24 h post transfection cells were lysed,  FLAG-tagged proteins immuno-isolated and analyzed by  western blotting (B), with 10% of the inputs loaded. The quantification of the  western blot (B, lower panel) shows the mean signal intensity normalized on the input of at least two independent experiments. Data points from individual experiments are indicated."
NCBI gene IDs:
"FLAG: 
Nup50: 10762"
Figure legend: "(G) Confocal microscopy images of nuclei  assembled for 120 min in mock depleted, Nup50 depleted (∆Nup50), and  Nup50 depleted Xenopus egg extracts supplemented with recombinant  Xenopus  wild-type  Nup50 or RCC1 binding mutants. Nuclei  were fixed in 4 % PFA and 0.5 % glutaraldehyde, stained for NPCs  (mAB414) and the chromatin (DAPI). Scale bar: 10 µm. Quantitation shows the average percentage of  mAB414 positive  nuclei for 100 randomly chosen chromatin substrates in each of three independent experiments. Individual data points are indicated (H) Confocal microscopy images of nuclei  assembled for 120 min in mock depleted, Nup50  depleted (∆Nup50), and Nup50 depleted Xenopus egg extracts supplemented with  RCC1  excess as indicated. Nuclei  were fixed in 4 %  PFA and 0.5 % glutaraldehyde,  stained for NPCs (mAB414) and the  chromatin (DAPI). Scale bar: 10 µm. Quantitation shows the average percentage of  mAB414 positive nuclei for 100 randomly chosen  chromatin substrates in each of three independent experiments. Individual data points are indicated. "
NCBI gene IDs:
"Nup50: 503671///503675"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. (A) The scatter plot shows the apparent Young's moduli obtained by AFM analysis. Bars represent the median values of the distributions. The immunoblot shows UBTD1 siRNA knock-down efficiency."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. (B) Immunoblots (left) and quantification (right) show levels of GST-ROCK associated GTP-bound RhoA (RhoA-GTP) and total RhoA in lysates (RhoA) in confluent cells. Immunoblot of UBTD1 shows the level of siRNA depletion."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. (C) Representative confocal images of focal adhesion (paxillin) and actin cytoskeleton (phalloidin) and outlines of focal adhesions using threshold-based image. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. (D) Cell area, number and area of focal adhesion were quantified (ImageJ) using the analysis as shown (C)."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. Sub-confluent DU145 (E) cells were seeded on two matrices of different stiffnesses (4 and 12 kPa). Representative heat map (left) and quantification (right) showing contractile forces generate by cells plated on 4kPa or 12kPa hydrogel."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "A549 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. Sub-confluent A549 (F) cells were seeded on two matrices of different stiffnesses (4 and 12 kPa). Representative heat map (left) and quantification (right) showing contractile forces generate by cells plated on 4kPa or 12kPa hydrogel."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. (G) Confluent DU145 cells were seeded on 12 kPa matrix. Representative heat map (left) and quantification (right) showing contractile forces generate by cells plated on 12kPa hydrogel."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool or siUBTD1single1 or single2) for 24 h and plated on fibronectin-coated glass coverslips. (H) Representative confocal images of YAP and UBTD1 localization on two matrices of different stiffness. DU145 cells were grown on collagen/acrylamide-coated glass coverslips (soft) or directly on glass coverslips (stiff). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "Proximal ligation assay monitoring (left) and quantification (right) of UBTD1 association with E-cadherin or β-catenin in DU145 cells transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "Immunoblots of UBTD1, β-catenin and YAP after subcellular fractionation of confluent DU145 cells transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool). EGFR, RhoGDI and Lamin A/C were used as controls for the membrane (M), cytosol (C) and nucleus (N) compartments, respectively."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected for 48h with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). (B) Immunoblots of p-YAP (ser127), YAP and UBTD1 (left) and quantification of YAP and p-YAP/YAP levels (right) in control or UBTD1-depleted DU145 at low and high cell density. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Actin was used as a loading controls. , immunoblots for the loading control (actin) and UBTD1 knock-down efficiency (UBTD1) are duplicated because they belong to the same experiment shown in both panels."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected for 48h with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). (C) Immunoblots of the Hippo signaling pathway using phosphorylated (p-ser909LATS and p-thr183MST1/p-thr180MST2) and total LATS, MST at low and high DU145 cells density. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Actin and Rho-GDI were used as loading controls. , immunoblots for the loading control (actin) and UBTD1 knock-down efficiency (UBTD1) are duplicated because they belong to the same experiment shown in both panels."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected for 48h with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). Immunoblots (up) and quantification (down) of YAP levels in confluent (D) DU145 UBTD1 depleted cells treated or not with cycloheximide (CHX) for 2h. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Actin was used as a loading control."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "A549 cells were transfected for 48h with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). Immunoblots (up) and quantification (down) of YAP levels in confluent (E) A549 UBTD1 depleted cells treated or not with cycloheximide (CHX) for 2h. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Actin was used as a loading control."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells or A549 cells were transfected for 48h with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). Immunoblots (up) and quantification (down) showing YAP levels in UBTD1 depleted confluent DU145 A549 cells treated or not with MG132 for 6h. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Actin was used as a loading control."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(C) Proximal ligation assay monitoring (left) and quantification (right) of UBTD1 associated with YAP of DU145 and A549 cells transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(E) Co-immunoprecipitation in confluent DU145 cells between endogenous β-TrCP, Ubch5c, YAP and β-catenin. DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool or siUBTD1single). β-TrCP was used as bait. IgG isotype was used as a negative control. Immunoblot of UBTD1 shows the level of siRNA-mediated depletion."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "Proximal ligation assay monitoring (F) of β-TrCP associated with Ubch5, YAP or β-catenin in DU145 cells transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "quantification (G) of β-TrCP associated with Ubch5, YAP or β-catenin in DU145 cells transfected with the indicated siRNA (control, siCTRL; UBTD1: siUBTD1pool)."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(H) Immunoblots show YAP ubiquitylation in HEK cells in different experimental conditions. Cells were transfected, as indicated, with expression vectors of histidine-tagged ubiquitin (His-Ub) together with control siRNA or UBTD1 siRNA. His-Ub crosslinked forms of YAP were purified (IP: His) and the immunoblot of YAP showed YAP ubiquitylation. The immunoblot of YAP (lower panel) was performed in parallel to verify the amounts of YAP protein engaged in the His-Ub purifications. The immunoblot of UBTD1 shows the level of siRNA depletion."
NCBI gene IDs:
"UBTD1: 80019
YAP: 10413"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). (A) Representative wide-field immunofluorescence images (left) and quantification (right) showing endogenous YAP nuclear translocation in DU145 cells after UBTD1 depletion. Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool). (B) Quantification of CTGF1, CYR61 and BIRC5 mRNA levels."
NCBI gene IDs:
"BIRC5: 332
CYR61: 3491
CTGF1: 1490
UBTD1: 80019"
Figure legend: "(C) Immunoblots of YAP in DU145 cells depleted (siUBTD1single2) or not (siCTRL) for UBTD1 and transfected or not with GFP-UBTD1 (+). Immunoblot of UBTD1 shows the level of siRNA-mediated depletion. Immunoblot of GFP shows the level of UBTD1 transfection. CHC was used as a loading control."
NCBI gene IDs:
"GFP: 
UBTD1: 80019"
Figure legend: "(D) Representative confocal immunofluorescence images (left) and quantification (right) showing endogenous YAP nuclear translocation in DU145 cells depleted (siUBTD1single2) or not (siCTRL) for UBTD1 and transfected or not with GFP-UBTD1. Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"GFP: 
UBTD1: 80019"
Figure legend: "(E) Immunoblots of p-YAP and YAP in DU145 control cells (siCTRL) or depleted for LATS1 and LATS2 (siLATS1+siLATS2) and/or UBTD1 (siUBTD1pool). Immunoblot of p-YAP reflects the activity of the Hippo pathway. Immunoblot of UBTD1 and LATS show siRNA knock-down efficiency. Actin was used as a loading control."
NCBI gene IDs:
"LATS1: 9113
LATS2: 26524
UBTD1: 80019"
Figure legend: "Representative confocal immunofluorescence images (F) showing endogenous YAP nuclear translocation in DU145 control cells (siCTRL) or depleted for LATS1 and LATS2 (siLATS1+siLATS2) and/or UBTD1 (siUBTD1pool). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"LATS1: 9113
LATS2: 26524
UBTD1: 80019"
Figure legend: "quantification (G) showing endogenous YAP nuclear translocation in DU145 control cells (siCTRL) or depleted for LATS1 and LATS2 (siLATS1+siLATS2) and/or UBTD1 (siUBTD1pool)."
NCBI gene IDs:
"LATS1: 9113
LATS2: 26524
UBTD1: 80019"
Figure legend: "(H) Immunoblots of YAP in DU145 control (siCTRL) or depleted for ROCK1 (siROCK1) or ROCK2 (siROCK2) and/or UBTD1 (siUBTD1pool). Immunoblots of UBTD1, ROCK1 and ROCK2 show the level of siRNA knock-down efficiency. Actin was used as a loading control."
NCBI gene IDs:
"ROCK1: 6093
ROCK2: 9475
UBTD1: 80019"
Figure legend: "Representative confocal immunofluorescence images (I) showing endogenous YAP localization in DU145 control cells (siCTRL) or depleted for ROCK1 (siROCK1) or ROCK2 (siROCK2) and/or UBTD1 (siUBTD1pool). Nuclei were stained with DAPI (Blue) on the MERGE image."
NCBI gene IDs:
"ROCK1: 6093
ROCK2: 9475
UBTD1: 80019"
Figure legend: "quantification (J) showing endogenous YAP localization in DU145 control cells (siCTRL) or depleted for ROCK1 (siROCK1) or ROCK2 (siROCK2) and/or UBTD1 (siUBTD1pool)."
NCBI gene IDs:
"ROCK1: 6093
ROCK2: 9475
UBTD1: 80019"
Figure legend: "(K) Quantification of CTGF1, CYR61, UBTD1 and BIRC5 mRNA in DU145 control cells (siCTRL) or depleted for ROCK1 (siROCK1) or ROCK2 (siROCK2) and/or UBTD1 (siUBTD1pool)."
NCBI gene IDs:
"BIRC5: 332
CYR61: 3491
CTGF1: 1490
ROCK1: 6093
ROCK2: 9475
UBTD1: 80019"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool or siUBTD1single1; UBTD1 +YAP+TAZ, siUBTD1single1+siYAPsingle1+ siTAZsingle1 or siUBTD1single1+siYAPsingle2+ siTAZsingle2). (A) Quantification of EMT marker genes (vimentin, slug, snail, fibronectin, α-SMA, E-cadherin) and UBTD1."
NCBI gene IDs:
"snail: 
α-SMA: 59
E-cadherin: 999
fibronectin: 2335
slug: 6591
UBTD1: 80019
vimentin: 7431
TAZ: 25937
YAP: 10413"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool or siUBTD1single1; UBTD1 +YAP+TAZ, siUBTD1single1+siYAPsingle1+ siTAZsingle1 or siUBTD1single1+siYAPsingle2+ siTAZsingle2). (C) Quantification of DU145 cell migration in Transwell chamber inserts."
NCBI gene IDs:
"UBTD1: 80019
TAZ: 25937
YAP: 10413"
Figure legend: "DU145 cells were transfected with the indicated siRNA (control, siCTRL; UBTD1, siUBTD1pool or siUBTD1single1; UBTD1 +YAP+TAZ, siUBTD1single1+siYAPsingle1+ siTAZsingle1 or siUBTD1single1+siYAPsingle2+ siTAZsingle2). (D) Quantification of DU145 cell invasion in Transwell chamber inserts."
NCBI gene IDs:
"UBTD1: 80019
TAZ: 25937
YAP: 10413"
Figure legend: "(E) Invasive properties of human prostate tumoroids depleted for UBTD1."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(F) Quantification of UBTD1 mRNA levels in prostate tumor (n=70) and adjacent normal tissues (n=47)."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(I) The levels of UBTD1 mRNA in grade 3 prostate adenocarcinoma patients (56 patients) are correlated with overall survival (OS). OS was calculated from patient subgroups with mRNA levels that were less or greater than the first quartile value."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "(J) The levels of UBTD1 mRNA in lung adenocarcinoma patients (522 patients) are correlated with OS."
NCBI gene IDs:
"UBTD1: 80019"
Figure legend: "Lipid hydrolyzing activity assayed in the presence of different phospholipids using purified PLDα6 from E. coli. Solid bars are activities assayed using purified PLDα6 whereas open bars were empty vector control that used an equal volume of eluents from bacteria containing the empty vector that was identically processed as those expressing PLDα6. Values are means ± SD (n = 3 biological replicates)."
NCBI gene IDs:
"PLDα6: "
Figure legend: "Total lipid levels in WT, pldα6 and COM without and with 10 μM GA3. Leaf samples (15 seedling each) from 4-leaf stage rice (Dongjin background) were collected and lipids were extracted and profiled using ESI-tandem mass spectrometry. Values are means ± SD (n = 3 biological replicates)). MGDG, monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; PA, phosphatidic acid."
NCBI gene IDs:
"pldα6: "
Figure legend: "Phospholipid species in WT, pldα6 and COM. Leaf samples (15 seedling each) from 4-leaf stage rice (Dongjin background) were collected and the different phospholipid species including carbon number and double bond number were determined. Values are means ± SD (n = 3 biological replicates). GA3 was dissolved in ethanol and seedlings treated with the same ethanol concentration were used as control."
NCBI gene IDs:
"pldα6: "
Figure legend: "PLDα6-GFP distribution in rice protoplasts with different concentrations of GA3 or IAA. Protoplasts from 12-day old rice (ZH11 background) leaf sheath tissue were collected. Rice protoplasts were transfected with pM999-PLDα6 for 12 hours. After that, GA3 or IAA was added to protoplasts and 2 hours later, confocal images of protoplasts are shown. pM999-GFP refers to the empty vector with GFP only that was transformed as control and Ghd7-RFP was a nucleus marker. Scale bar = 10 μm."
NCBI gene IDs:
"PLDα6: "
Figure legend: "Subcellular location of GID1 in WT and pldα6 protoplasts with or without GA3 treatments. Cells for 12 hours after transformation were treated with or without 10 μM GA3 for two hours. Bars = 10 μm."
NCBI gene IDs:
"pldα6: "
Figure legend: "Immunoblotting of subcellular fractions of GID1 expressed in WT and pldα6 protoplasts. After 2-hour treatment with 10 μM GA3, total (T), soluble (S) and nuclear (N) protein fractions were isolated from 10 samples of protoplasts. Equal amount of each sample was loaded for SDS-PAGE and immunoblotting."
NCBI gene IDs:
"pldα6: "
Figure legend: "Protoplasts of WT and pldα6 from 12-day old stage leaf sheath were collected and transfected with a pM999-SLR1 construct, incubated for 12 hours and then treated with 10 μM GA3 for 0, 3, and 9 hours. The first column, fluorescence from GFP; the second, red fluorescence from the nucleus marker Ghd7; the third, bright field, and the fourth, overlay of the three channels. All confocal images were scanned using similar laser gain and offset settings. Bars = 10 μm."
NCBI gene IDs:
"pldα6: "
Figure legend: " (f) GFP in vivo fluorescence post intradermal injection of chitin oligomers into LysMEGFP/+ C57BL/6 mice (n=3/group) Data information represent data (mean+SEM) combined from 'n' (given in brackets for each panel) technical or biological replicate mic"
NCBI gene IDs:
"LysM: 17105"
Figure legend: "TNF released from WT or Myd88 (A KO BMDM (total n=6-9/group combined from 3 experiments) Data information: I represent data (mean+SEM) combined from 'n' technical or biological replicate mic Student' t-test"
NCBI gene IDs:
"Myd88: 17874"
Figure legend: "TNF released from W or Tlr KO BMDM (total n=6-9/group combined from 3 experiments) Student's t-tes"
NCBI gene IDs:
"Tlr: 24088"