EMBO/SourceData huggingface dataset:
Figure legend: "A-D, Single (A), double (B), triple (C), and quadruple (D) cysteine mutant NDP52  plasmids were generated and transfected into HeLa PentaKO cells. Cells were treated with 20 µM  PR-619  for 10 min and analysed by immunoblotting  for NDP52  in either reducing  or non-reducing conditions. The constructs highlighted in red were subjected to the next round of Cys-Ala screen or further analyses."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "A, B, Fluorescence microscopy images (A) and quantification (B) of mitophagy  of HeLa  wild-type (WT),  PentaKO + empty vector, PentaKO + NDP52  WT or NDP52  C18,153,163,321S (Mut) cells, stably expressing YFP-Parkin    and mt-mKeima    were pre-treated with or without MitoQ for 22 h and treated with 4 µM / 10 µM AO for the indicated times. Data information Data are mean ± s.e.m. (B or displayed as cell popular violin plots (B). P values were calculated by one-way ANOVA followed by Sidak test on three independent experiments (B *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §, p&lt;0.05, §§§, p&lt;0.001 (relative to MitoQ-untreated condition); ns (non-significant). Scale bars: 20 μm (A"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "C, HeLa PentaKO + NDP52  WT or NDP52  Mut cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 4 µM / 10 µM AO for 2 h in the absence or presence of 10 μM MG132  (MG) and 400 nM Baf, followed by a mitochondrial  fractionation and immunoblotting  for NDP52  in non-reducing conditions. The mitochondrial  protein UQCRC2  was used as a loading control. Data information: #, non-specific band or a post-translational modification."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "D, HeLa PentaKO + NDP52  WT cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 1 mM DFP  for 24 h (D), or pre-treated with 500 nM MitoQ  for 22 h and treated with 4 µM / 10 µM AO for 2 h (D Cells were then analysed by MitoSOX staining  (D) Data information: #, Data are mean ± s.e.m. D) P values were calculated by one-way ANOVA followed by Sidak test on three independent experiments D). *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §, p&lt;0.05, §§§, p&lt;0.001 (relative to MitoQ-untreated condition); ns (non-significant). Scale bars: 20 μm D)."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "E, HeLa PentaKO + NDP52  WT cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 500 nM MitoQ  for 22 h and treated with 4 µM / 10 µM AO for 2 h E) in the presence or absence of 400 nM Baf  (E). immunoblotting  for NDP52  (E). Data information: #, non-specific band or a post-translational modification."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "A, Fluorescence microscopy  images and quantification of mitophagy  in HeLa PentaKO + NDP52  WT cells stably expressing YFP-Parkin    and mt-mKeima    were treated with 4 µM rotenone  (R), 4 µM A, 10 µM O, or the combination of RO or AO for 3h. Data information Data are mean ± s.e.m. (A or displayed as cell popular violin plots (A, P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on at least three independent experiments as indicated (A, *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ns (non-significant). Scale bars: 20 μm (A"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "B, Fluorescence microscopy  images and quantification of MitoSOX staining  in HeLa PentaKO + NDP52  WT cells in the same conditions as (A). Data information Data are mean ± s.e.m. B, or displayed as cell popular violin plots B, P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on at least three independent experiments as indicated B, *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ns (non-significant). Scale bars: 20 μm B,"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "HeLa PentaKO + NDP52  WT cells stably expressing YFP-Parkin  and mt-mKeima  (C, were pre-treated with or without 10 µM S1QEL  or 20 µM S3QEL  for 30 min and treated with AO for 2 h or with RO for 3 h, followed by mitophagy  measurement (C) Data information Data are mean ± s.e.m. C, or displayed as cell popular violin plots C, P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on at least three independent experiments as indicated C, *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ns (non-significant). Scale bars: 20 μm"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "HeLa PentaKO + NDP52  WT cells (D) were pre-treated with or without 10 µM S1QEL  or 20 µM S3QEL  for 30 min and treated with AO for 2 h or with RO for 3 h, followed by MitoSOX staining  (D) Data information Data are mean ± s.e.m. P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on at least three independent experiments as indicated *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ns (non-significant). Scale bars: 20 μm , D"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "HeLa PentaKO + NDP52  WT cells stably expressing YFP-Parkin  and mt-mKeima  E) were pre-treated with or without 10 µM S1QEL  or 20 µM S3QEL  for 30 min and treated with AO for 2 h or with RO for 3 h, followed by immunoblotting  for NDP52  in non-reducing conditions (E). Data information: #, non-specific band or a post-translational modification."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "F, HeLa  PentaKO + NDP52  WT stably expressing YFP-Parkin  and mt-mKeima  were pre-treated with or without 10 mM DTT  for 30 min and treated with AO for 2 h followed by mitophagy  measurements (F) Data information Data are mean ± s.e.m. F) or displayed as cell popular violin plots F). P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on at least three independent experiments as indicated F). *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ns (non-significant). Scale bars: 20 μm F)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "G, HeLa PentaKO + NDP52  WT stably expressing YFP-Parkin  and mt-mKeima  were pre-treated with or without 10 mM DTT  for 30 min and treated with AO for 2 h followed by immunoblotting  for NDP52 in non-reducing conditions (G). Data information: #, non-specific band or a  post-translational modification."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "HeLa PentaKO + NDP52  WT or NDP52  Mut cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 4 µM / 10 µM AO for 2 h in the presence or absence of 400 nM Baf, followed by immunofluorescence  analyses (A) The number of foci of the indicated proteins colocalised  with NDP52, or foci of NDP52 colocalised  with Parkin, was quantified. Data information: Data are mean ± s.e.m. (A, P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on three independent experiments (A *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §§, p&lt;0.01; §§§, p&lt;0.001 ns (non-significant). Scale bars: 20 μm (A,"
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "HeLa PentaKO + NDP52  WT or NDP52  Mut cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 4 µM / 10 µM AO for 2 h in the presence or absence of 400 nM Baf, followed by immunoblotting  for LC3   and NDP52  in mitochondrial  fraction (B) or  whole cell lysate (C) in reducing conditions Data information: Data are mean ± s.e.m. B, C, P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on three independent experiments B, C *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §§, p&lt;0.01; §§§, p&lt;0.001 ns (non-significant)."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "HeLa PentaKO + NDP52  WT or NDP52  Mut cells stably expressing YFP-Parkin  and mt-mKeima  were treated with 4 µM / 10 µM AO for 2 h in the presence or absence of 400 nM Baf, followed by co-immunoprecipitation  assay to analyse the interaction  of NDP52  with FIP200, ULK1  and ATG13  (D). Data information: Data are mean ± s.e.m. P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on three independent experiments D, *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §§, p&lt;0.01; §§§, p&lt;0.001 ns (non-significant)."
NCBI gene IDs:
"mKeima: 
YFP: 
NDP52: 10241
Parkin: 5071"
Figure legend: "E, MEFs  stably expressing YFP-Parkin, mt-mKeima  and empty, human NDP52  (hNDP52) WT or hNDP52 Mut were pre-treated with or without 500 nM MitoQ  for 21 h and treated with AO for 3 h followed by mitophagy  measurement. Data information: Data are mean ± s.e.m. E) or displayed as cell popular violin plots (E). P values were calculated by one-way ANOVA followed by Sidak test (for multiple groups) or unpaired two-tailed Student's t-test (for two groups) on three independent experiments E). *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; §§, p&lt;0.01; §§§, p&lt;0.001 (relative to MitoQ-untreated condition); ns (non-significant). Scale bars: 20 μm E)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "B. HoxB8 macrophages  were treated with the indicated dilutions of  GM-CSF using supernatants from GM-CSF  expressing cells. Shown are ELISA analysis of  IL-1β secretion in supernatants and  western blots  indicating IL-1β  expression in lysates.. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "C. HoxB8 macrophages  of the indicated genotypes were treated as indicated with 60ng/mL UP-LPS, 1%  GM-CSF containing supernatant (approximately 2ng/mL GM-CSF), 40µM WEHD.FMK, 0,5µM  CRT-0066101  for 16 hours, 5µM nigericin was added for the last hour. Supernatants were analysed by ELISA  for IL-1β. Inlay panels show western blots for levels of IL-1β  expression in the indicated knockouts. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "D. HoxB8 cells were treated with 60ng/mL of UP-LPS, 1%  GM-CSF supernatant, 50mM KCl, and 5µM nigericin  as indicated for 16 hours before supernatants were analysed for  IL-1β by ELISA. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "A. BMDMM  (left panel) and HoxB8 macrophages  (right panel) were treated as indicated with either LPS  or LPS+ GM-CSF. Cells were harvested and proteins extracted and analysed by western blot for levels of NRF2. Shown is the densitometric  quantification of Nrf2  protein level from three independent experiments. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "B. HoxB8 macrophages  were treated as in A. and levels of  IL-1β in the supernatant were analysed by ELISA  Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "C. HoxB8 macrophages were treated for 12 hours with LPS  or LPS+GM-CSF and RNA extracted and analysed by qPCR  for the indicated targets. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "D. Wild type and Tnf-/- HoxB8  cells were treated as indicated. Cells were harvested and proteins extracted and analysed by western blot for levels of NRF2  and HMOX1. For wild type cells,  MitoTEMPO (50µM) was also added as indicated. Shown are the densitometric  quantifications of NRF2  and HMOX1  protein levels of three independent experiments (right panel). Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416
Tnf: 21926"
Figure legend: "E. HoxB8  cells of the indicated genotypes were treated with LPS  for 8 hours followed by staining  with mitoSOX red. The fluorescent signal was analyzed using flow cytometry. Shown are average geometric mean fluorescence intensity of mitoSOX red. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "F. Tnf-/- HoxB8 macrophages  were treated with LPS  with or without 50ng/ml recombinant TNF  for 16 hours. Cells were harvested and proteins extracted and analysed by western blot  for levels of NRF2  and HMOX1  (upper panel). Shown are the densitometric  quantifications of indicated protein levels of three independent experiments (lower panel). Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. For the western blot quantification, significance was calculated using unpaired t-test. p values are shown."
NCBI gene IDs:
"HoxB8: 15416
Tnf: 21926"
Figure legend: "A. HoxB8 macrophages  were seeded in an 8-well Ibidi chamber and treated as indicated for 12 hours. Cells were fixed and stained  for ASC  (Red) and with DAPI  (blue). For nigericin  treatments nigericin  was added for only 30 minutes. Example cells containing ASC  Specks are shown with white arrows. Scale bars represent 50µM. Data information: Microscopy  pictures are representative images from 3 biological replicates."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "B. HoxB8  cells were treated as indicated with LPS  or LPS+GM-CSF. The cross linker DSS  was added to the cells as described in methods. Samples were analysed by western blot  for ASC oligomerization. Black vertical lines indicate that non-relevant lanes were removed during figure preparation but samples were run on the same gel. Data information: westerns  are representative images from 3 biological replicates."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "C. Wild type and caspase-1-/- HoxB8 macrophages were treated as indicated for 16 hours. Cell lysates were made and analysed by  western blot for levels of  caspase-1  and gasdermin D cleavage. Data information: westerns  are representative images from 3 biological replicates."
NCBI gene IDs:
"caspase-1: 12362
HoxB8: 15416"
Figure legend: "D. HoxB8 macrophages  were treated as indicated with LPS  or LPS+GM-CSF for 12 hours. Cells were lysed and NLRP3  was  immune precipitated using a magnetic bead coupled antibody. As a negative control, beads without NLRP3  antibody were used. Beads were boiled and analysed for recruitment of NLRP3, and caspase-1. * indicates light chain from the antibody. Molecular weight markers shown in middle of NLRP3  blot were embedded in image using Chemostar imaging software (INTAS) at time of image acquisition. Data information: westerns  are representative images from 3 biological replicates."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "E. HoxB8 macrophages  were incubated with combinations of LPS, GM-CSF, nigericin  +/- Z-VAD.FMK as indicated. Proteins were extracted and analysed by western blot  for caspase-1 processing. Data information: westerns  are representative images from 3 biological replicates."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "F. HoxB8 macrophages  were seeded in a 96 well microplate and co-treated with either 10µM or 50µM  z-VAD with LPS+GM-CSF  for 16 hours. Then 100nM of Sytox Green  was added to the cells. The number of sytox green+ cells per image was quantified. Data information: Significance was calculated using one way ANOVA with multiple comparisons. Every dot represents one biological replicate. P values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "A. HoxB8 macrophages  were treated for 16 hours with LPS  +/- GM-CSF as indicated. Proteins were extracted and analysed for levels of  IL-1β by western blot."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "B. HoxB8 macrophages  were treated with LPS  or LPS+GM-CSF as indicated for 16 hours. Cells were lysed in buffer containing FLAG  tagged K63 TUBE  and K63 ubiquitylated  proteins were precipitated using anti FLAG pull down. Pulled down  proteins were analysed for  IL-1β and ubiquitin   by western blot."
NCBI gene IDs:
"FLAG: 
HoxB8: 15416"
Figure legend: "C. IL-1β-/- hoxB8 macrophages  were infected with lentivirus  expressing IL-1β-FLAG-6xHis or the indicated mutants from the  IL-1β promoter. Cells were treated as indicated with LPS or LPS+GM-CSF or LPS+Nigericin and levels of secreted  IL-1β were analysed by ELISA  (top panel). GM-CSF treated cells were also lysed in urea buffer and nickel  NTA affinity pull down was performed. Nickel  NTA pull down was also performed on supernatants (bottom panel). Pulled down proteins form the His tag pull down were analysed for the presence of  IL-1β by western blot. Molecular weight markers shown in middle of blot  were embedded in image using Chemostar imaging software (INTAS) at time of image acquisition. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"FLAG: 
His: 
hoxB8: 15416
IL-1β: 16176"
Figure legend: "D. HoxB8 macrophages  were treated as in A with addition of 50µM mitoTEMPO or 10mM NAC as indicated. Proteins were extracted and analysed for  IL-1β by  western blot."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "E. Wild type and TNF-/- HoxB8 macrophages  were treated as indicated with LPS  or LPS+GM-CSF. Proteins were extracted and analysed for  IL-1β by  western blot. Black vertical lines indicate that non-relevant lanes were removed during figure preparation but samples were run on the same gel."
NCBI gene IDs:
"HoxB8: 15416
TNF: 21926"
Figure legend: "F. HoxB8 macrophages  were treated as in A but with the addition of 25µM Punicalagin  throughout the 16 hour time course. Proteins were extracted and analysed by western blot (left panel). Supernatants were also analysed by ELISA for secreted IL-1β (right panel). Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "A. Wild type and GsdmD-/- HoxB8 macrophages  were treated as indicated for 16 hours. Supernatants were analyzed for IL-1β secretion by ELISA. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"GsdmD: 69146
HoxB8: 15416"
Figure legend: "B. HoxB8 macrophages  were seeded in an 8-well Ibidi chamber and treated for 14 hours with LPS  and  GM-CSF. Cells were fixed and stained  for GasderminD  (green),  IL-1β (red) and DAPI (blue). Images were taken at the Zeiss LSM880 Confocal microscope Data information: shown. Microscopy  pictures are representative images from 3 biological replicates. Scale bars in microscopy  images represent 10µM."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "C. HoxB8 macrophages were co-stimulated with three different PEG  3mM for 16 hours as indicated. Supernatants were analyzed for IL-1β secretion by ELISA. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "D. HoxB8 macrophages  were seeded in 96 well microplate and treated as indicated. Before treatment, sytox green was added and cells were imaged in an incucyte incubator microscope  every hour for 16 hours. Shown is the sytox green signal normalized to the total cell area. Data information: n≥ 3 biological replicates (every dot represents one biological replicate). For all panels, error bars are SEM. Significance was calculated using one way ANOVA with multiple comparisons, or two-way ANOVA to compare across genotypes. p values are shown."
NCBI gene IDs:
"HoxB8: 15416"
Figure legend: "E. Wild type and GsdmD-/- HoxB8 macrophages were treated as indicated for 16 hours. Proteins were extracted and analysed for  IL-1β ubiquitination  by western blot. Data information: westerns  are representative images from 3 biological replicates."
NCBI gene IDs:
"GsdmD: 69146
HoxB8: 15416"
Figure legend: "(D) Using the dual color labeling system (esg-Gal4&gt;UASp-FRT-H3-eGFP-FRT-H3-mCherry), old and new H3   distribution in prophase  and prometaphase ISCs  shows large, separable domains of H3-eGFP  (old) and H3-mCherry  (new). Note: all figure panels in this work are maximum intensity projection images to show signals from all Z-stacks. (E) Using the UASp-FRT-histone-eGFP-T2A-histone-mCherry-PolyA  transgene that co-expresses H3-eGFP    and H3-mCherry, significant overlap between the two signals were detected in ISCs  during prophase  and prometaphase. (F) Quantification of the colocalization  between old and new H3, as well as between the co-expressed eGFP- and mCherry-tagged H3    in prophase  and prometaphase ISCs. Pearson's correlation coefficients were measured, where 1 represents complete colocalization  and 0 stands for no colocalization. Old and new H3   showed significantly less colocalization  (Pearson's correlation coefficient for H3   = 0.44 + 0.02, n = 81 ISCs) when compared to the co-expressed eGFP- and mCherry-tagged H3   (Pearson's correlation coefficient for H3  co-expressed = 0.86 + 0.01, n = 19 ISCs). Individual data points and mean values are shown. Error bars represent SEM. **** p &lt; 0.0001; unpaired t test to compare two individual datasets to each other. Data information: n for individual ISCs. Scale bar in (D), (E): 5 µm."
NCBI gene IDs:
"eGFP: 
histone: 
mCherry: 
esg: 34903
Gal4: 855828
H3: 3771723"