EMBO/SourceData huggingface dataset:
Figure legend: "(B) Knockdown of IP3R1 prevents ER stress-induced apoptosis in EI24 knockout cells. Cells were cultured with or without 4 μM TM for 8 hr. Percentages of early apoptotic cells were measured by  flow cytometry. Data information: N ≈ 10000 cells for one biological replicate. Data are mean ± SEM (n = 3 or 4 biologically independent replicates). ns, no significant difference; ***P &lt; 0.001; two-tailed Student's t-test"
NCBI gene IDs:
"EI24: 9538
IP3R1: 3708"
Figure legend: "(D) Knockdown of  IP3R1 prevents ER stress-induced apoptosis. Cells were treated with 4 μM TM  for the indicated times. Protein expressions were analyzed by  immunoblotting."
NCBI gene IDs:
"IP3R1: 3708"
Figure legend: "(F-H) Reintroduction of full-length EI24, but not the TM34- or C-terminus-deleted mutant (F, G) or the C-terminus alone (H), inhibited ER stress-induced apoptosis in EI24  knockout cells. Cells treated with or without  TM were analyzed for apoptosis  by  flow cytometry (F, H) or by  immunoblotting (G). Arrows in (H) denote decreased or increased apoptotic levels. Data information: N ≈ 10000 cells for one biological replicate. Data are mean ± SEM (n = 3 or 4 biologically independent replicates). ns, no significant difference; ***P &lt; 0.001; two-tailed Student's t-test for F), one-tailed Student's t-test for (H)."
NCBI gene IDs:
"EI24: 9538"
Figure legend: "(A-B) Levels of miR-122 in Huh7 cells and released EVs either untreated (Fed) or subjected to starvation for metabolites including amino acids for 16h (Starved). miR-122 signals were detected by Northern blotting and position of the 32P-labelled Oligos that served as size markers are shown in the lane (A). U6 snRNA was used as loading control."
NCBI gene IDs:
"U6: 
miR-122: 406906"
Figure legend: "(A-B) Levels of miR-122 in Huh7 cells and released EVs either untreated (Fed) or subjected to starvation for metabolites including amino acids for 16h (Starved). Cellular CAT-1 levels were measured by qRT-PCR using GAPDH mRNA as control (B). A scheme of experiment is shown in the top panel in B."
NCBI gene IDs:
"GAPDH: 
CAT-1: 6541"
Figure legend: "(C-D) Cellular and extracellular levels of different miRNAs and EV associated proteins in Fed and Starved Huh7 cells. Relative changes in cellular and extracellular levels (EV associated) of miR-122 and miR-24 in Fed or 8h Starved Huh7 cells were quantified by qRT-PCR and plotted (mean+/- s.e.m., n=3). Levels of individual miRNAs in Fed condition were taken as unit. Fold change (with SD) in the cellular level of all miRNAs and pre-miR-122 measured were shown in the bottom panel (C). Cellular miRNA levels were normalized against U6 snRNA."
NCBI gene IDs:
"U6: 
miR-122: 406906
miR-24: 407013///407012"
Figure legend: "(E) Effect of GW4869 treatment on cellular miRNA content in Fed and Starved cells. Levels of miRNAs were measured by real-time quantification and normalized against U6 snRNA. Mean data are from three independent experiments. DMSO treatment was used as control for GW4869 treated cells."
NCBI gene IDs:
"U6: "
Figure legend: "(F) CAT-1 and Aldolase mRNA expression in DMSO or GW4869 treated starved Huh7 cells. qRT-PCR techniques was adopted for quantification using 18S rRNA values for normalization (mean +/- s.e.m., n=3)."
NCBI gene IDs:
"18S: 
Aldolase: 229///226
CAT-1: 6541"
Figure legend: "(G) Effect of siSMPD2 treatment on miR-122 content of EVs from Fed and Starved Huh7 cells. EVs from Fed and Starved Huh7 cells, depleted for SMPD2, were isolated and miR-122 content were measured by qRT-PCR and normalized against protein content of EVs. Fold change in EV associated miR-122 levels upon starvation both for control and siSMPD2 treated cells were shown above the respective bars."
NCBI gene IDs:
"miR-122: 406906
SMPD2: 6610"
Figure legend: "(H) Effect of siSMPD2 treatment on CAT-1 mRNA content (left panel) and miR-122 levels (right panel) in Huh7 cells. RNAs, from Fed and Starved Huh7 cells, depleted for SMPD2, were isolated and miR-122 and CAT-1 mRNA contents were measured by qRT-PCR. miR-122 contents were normalized against U6 snRNA. GAPDH mRNA was used for normalization of CAT-1 mRNA levels (mean +/- s.e.m., n=3). miR-122 levels for siCon treated cells (Fed and Starved) were considered as units."
NCBI gene IDs:
"GAPDH: 
U6: 
miR-122: 406906
CAT-1: 6541
SMPD2: 6610"
Figure legend: "(I-J) A schematic representation of the experiment to separate Extracellular Vesicles (EVs) on OptiPrep@ density gradient (left panel). Densities of fractions 1-10 are plotted and a best-fit curve is drawn (right upper panel). CD63 levels in individual fractions of Fed and Starved cells were detected by western blots to confirm the presence of exosomes of Fed and Straved cells (right lower panel). Mean Ct values of miR-122 in RNAs isolated from Exosome enriched (8-9) vs Non-exosomal (1-3) fractions were analyzed and plotted (J) (mean +/- s.e.m., n=3)."
NCBI gene IDs:
"miR-122: 406906"
Figure legend: "(L) Effect of thapsigargain (TG) treatment of Huh7 cells on cellular and EV associated miR-122 level. A schematic representation of the experiment (upper panel). miR-122 levels were measured by qRT-PCR in total cellular RNA and in EVs released by the treated cells. Values were normalized either against U6 snRNA or protein content of EVs (mean+/- s.e.m., n=3) (lower panels) for cellular and EV associated RNA respectively."
NCBI gene IDs:
"U6: 
miR-122: 406906"
Figure legend: "(A) Relative amount of miRNAs bound to HuR that are immunoprecipitated either from Fed or Starved Huh7 cells. Immunoprecipitated materials were separated into two equal halves to do western blot analysis of HuR and relative quantification of associated miR-122 and miR-21 were done by quantitative RT-PCR (mean +/- s.e.m., n=3). GFP used as negative control to confer the specificity of the anti-HuR3A2 antibody in immunoprecipitating HuR. The miR-122 content in the GFP-antibody immunoprecipitated materials was too low to get detected reliably using the qRT-PCR."
NCBI gene IDs:
"miR-122: 406906
miR-21: 406991"
Figure legend: "(B-C) Effect of HuR depletion on cellular and EV content of miRNAs in Huh7 cells. Schemes of HuR depletion experiments in Huh7 cells (B). In siHuR treated cells, depletion of HuR was monitored by western blotting (mean+/- s.e.m., n=4)"
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(B-C) Effect of HuR depletion on cellular and EV content of miRNAs in Huh7 cells. Relative levels of miRNAs were measured in EVs released from siCon or siHuR treated cells by qRT-PCR. Cellular miRNA contents were normalized against U6 snRNA. Levels of each miRNA in control siRNA (siCon) treated set were taken as unit. (C). CAT-1 and Aldolase mRNA levels of siHuR and siCon treated Fed Huh7 cells were quantified by qRT-PCR and normalized against GAPDH mRNA."
NCBI gene IDs:
"GAPDH: 
U6: 
Aldolase: 229///226
HuR: 1994
CAT-1: 6541"
Figure legend: "(D) Effect of HuR knockdown on cellular level of different miRNAs in starved Huh7 cells. Relative levels were estimated by real time qPCR, normalized with respect to U6 snRNA and plotted in the left panel. Levels of miR-122 in EVs of starved siHuR and siCon treated Huh7 cells were also measured by RT-PCR and plotted in the right panel (mean+/- s.e.m., n=3)."
NCBI gene IDs:
"U6: 
HuR: 1994
miR-122: 406906"
Figure legend: "(E) Level of eIF-2a and its phosphorylated form, estimated by western blot using specific antibodies, in siCon or siHuR treated and starved Huh7 cell extracts. Western blot data of HuR confirm reduction of this protein upon siRNA treatment. CAT-1 and Aldolase mRNA levels were estimated by real time quantification and normalized against GAPDH level. Values of siCon treated samples were taken as unit (mean+/- s.e.m., n=3). In the right panel the relative quantity of phosphorylated eIF-2a measured by densitometry were plotted. Levels of p38 and phosphor-p38 levels in siCon and siHuR treated Starved Huh7 cells were detected by western blot."
NCBI gene IDs:
"GAPDH: 
Aldolase: 229///226
HuR: 1994
CAT-1: 6541"
Figure legend: "(F) Western blot analysis to detect the level of phosphorylated eIF-2a and eIF-4E-BP1 in lysates of Starved and HuR depleted Huh7 cells either transfected with ant-let-7a or anti-miR-122 oligos. miR-122 inactivation was monitored by measuring cat-1 level by qRT-PCR using GAPDH as control (mean+/- s.e.m., n=3). * denotes the phosphorylated eIF-4E-BP1. b-Actin was used as loading control. Relative levels of phosphorylated eIF-2a were measured by densitometric estimation of three independent measurements."
NCBI gene IDs:
"GAPDH: 
HuR: 1994
miR-122: 406906
let-7a: 406881
cat-1: 6541"
Figure legend: "(A) Northern blot of miR-122 in Huh7 cells expressing the Control and HA-HuR expression plasmids. The U6 was used for loading control. Position of the 32P-labelled Oligos, blotted to the membrane used for Northern blotting, served as size markers are shown in the M lane."
NCBI gene IDs:
"U6: 
HuR: 1994
miR-122: 406906"
Figure legend: "(B) Schematic representation of experiments done in Huh7 cells expressing HA-HuR (bottom). For control, pCIneo transfected Huh7 cells were used. Relative expression of HuR in transfected cells were analysed by western blot (top). b-actin blots were used as loading controls."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(C) Cellular and EV-associated miRNA levels were measured in Huh7 cells either expressing the pCIneo or HA-HuR expressing vectors by qRT-PCR (mean+/- s.e.m., n=5). Cellular miRNA levels were normalized against U6 snRNA."
NCBI gene IDs:
"U6: 
HuR: 1994"
Figure legend: "(E) Effect of HA-HuR expression on miRNA levels on EV and non-EV fractions of the culture supernatant collected from control and HA-HuR encoding plasmid transfected Huh7 cells. For isolation of non-EV fraction, supernatant obtained after removal of EVs by ultracentrifugation was used for RNA preparation (left panel) (mean+/- s.e.m., n=3). Effect of HuR expression on CD63, Alix and HuR levels in EVs. Extracts from EVs isolated from control or HA-HuR expressing Huh7 cells were western blotted for respective proteins (right panel)."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(F) Relative miR-122 levels in CD63 positive affinity purified EVs isolated from HA-HuR and pCIneo transfected Huh7 cells were quantified by qRT-PCR (mean+/- s.e.m., n=3). The levels of miR-122 were normalized against CD63 content of EVs."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(G) Effect of GW4869 on EV associated content of three different miRNAs in HA-HuR expressing Huh7 cells quantified by qRT-PCR (mean+/- s.e.m., n=5). EVs from DMSO treated cells were used as control."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(H) A schematic representation of the experiment done to characterize EVs isolated from Huh7 cells expressing HA-HuR or pCIneo respectively by running OptiPrepTM density gradient (left panel) and western blotting for CD63 (right upper panel). Relative miR-122 levels in fractions 1-2 and 8-9 were compared by qRT-PCR (right lower panel)."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(I) Levels of apoptic cells in HA-HuR expressing Huh7 cells. Western Blot detection of apoptosis marker Cytochrome C was done (top) for EV associated and cellular fractions of HA-HuR and pCIneo (control) expressing Huh 7 cells respectively. Western blot for HA-HuR was done to analyse the expression of HA-HuR in EVs isolated from HA-HuR and pCIneo expressing Huh7 cells. CD63 and β-actin are used as loading control for EV associated fractions and cellular fractions respectively (upper panels). TUNEL assay of HA-HuR and pCIneo expressing and non-transfected Huh 7 cells was performed and representative pictures of HA-HuR and pCIneo expressing cells along with picture of non-transfected but DNase treated (positive control ) cells were compared (lower panels). Scale bar 50 µM."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(B) Change in miR-122 level in starved mouse liver. Endogenous miR-122 (left panel) and CAT-1 mRNA (middle panel) levels in mouse liver starved for 12h were quantified by qRT-PCR. miR-122 levels in the blood serum of corresponding animals were also measured (right panel). The Ct values of miR-122 and CAT-1 mRNA obtained for each individual animal liver and blood serum were plotted (mean+/- s.e.m., n=5)."
NCBI gene IDs:
"miR-122: 387231
CAT-1: 11987"
Figure legend: "(F). Change in miR-122 level upon expression of HA-HuR in mouse liver. Endogenous miR-122 level in HA-HuR expression plasmid injected mouse liver were quantified by RT-PCR and compared against liver RNA samples collected from control (pCIneo injected) group of animals (mean+/- s.e.m., n=5)."
NCBI gene IDs:
"HuR: 15568
miR-122: 387231"
Figure legend: "(G) Endogenous miR-122 target CAT-1 (left panel) and Aldolase (right panel) mRNA levels in HA-HuR or pCIneo (control) injected mouse liver were quantified by RT-PCR (mean+/- s.e.m., n=4)."
NCBI gene IDs:
"Aldolase: 230163///11674
HuR: 15568
miR-122: 387231
CAT-1: 11987"
Figure legend: "(H) miR-122 levels in the blood serum of pCIneo (control) or HA-HuR expression plasmid injected mice were measured. The Ct values of miR-122 and CAT-1 and Aldolase mRNAs obtained for each individual animal liver and blood serum were plotted (mean+/- s.e.m., n=5)."
NCBI gene IDs:
"Aldolase: 230163///11674
HuR: 15568
miR-122: 387231
CAT-1: 11987"
Figure legend: "(A) Time course experiment of HuR binding of miR-122 replaced from Ago2miRNPs. A schematic representation of in vitro miRNA binding assay of HuR (left panel). Equal amounts of recombinant HuR (rHuR), after indicated time of the miRNP interaction on FLAG-beads, were immunoprecipitated and HuR associated miR-122 levels were detected by qRT-PCR (right top panel). Ago2western blot detects its presence in the FLAG beads used in the assay and its absence in HuRimmunoprecipitated materials. HuRwestern blot was used to confirm its presence and quantification of its levels in the immunoprecipitated materials, at different time points (right bottom panel)."
NCBI gene IDs:
"miR-122: 406906"
Figure legend: "(C) Coomassie stained gel picture denotes the purity of the recombinant HuR used in the assay. The same proteins were western blotted for HuR to show the specificity of the antibody used for immunoprecipitation. The recombinant P protein and N protein of Chandipura virus was used as negative control to confirm the specificity of 3A2 anti-HuR antibody."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(D) HuR western blot in middle right panel confirmed the presence of HuR and the truncated version of it in the immunoprecipitated materials. A representative domain picture of HuR and HuRΔIII are shown in the upper panel. Relative levels of HuR bound miR-122 were determined by qRT-PCR (mean+/- s.e.m., n=3) (middle left panel). Amount of miR-122 recovered from the immunoprecipitated materials were normalized against the amount of HuR or HuRΔIII present in the Immunoprecipitated materials. Ago2 western blot confirmed its presence in the FLAG beads used in the assay while Ago2 was not detected in HuR immunoprecipitated materials (bottom panel)."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(E) RNA electrophoretic mobility shift assay done with 32P-labeled synthetic miR-122 and recombinant HuR proteins. Radiolabelled miR-122 (100 fmol) was incubated with increasing concentration of FL-HuR (5-100nM) or HuRΔIII (100 nM) and gel shifting of miR-122 were marked by arrowhead. Position of the free probes is marked by an arrow. In the right panel, 1pmol of unlabelled TNF-alpha AU-rich element encoding synthetic RNA was used to compete with miR-122 binding of full length HuR. Amount of HuR or HuRΔIII used for gel shift assay were premeasured by densitometric estimation of coomassie stained gel bands from SDS page."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(A) Subcellular distribution of HuR and Ago2 in Huh7 cells. Isotonic lysates of Huh7 cells transfected with HA-HuR expression or pCIneo control vector were analyzed on 3-30% OptiprepTM gradients for separation of organelles and localization of Ago2 and HuR were determined by western blotting analysis. Alix and Calnexin were used as markers of late endosome/MVB and ER respectively. Positions of specific organelle containing fractions were marked above the panels. * denotes the position of the high molecular weight HuR bands detected in the MVB fractions."
NCBI gene IDs:
"HuR: 1994"
Figure legend: "(C) Changes in relative level of miR-122 in the subcellular ER and MVB associated fractions of OptiprepTM gradients done with the cell lysates from pCIneo or HA-HuR expression vector transfected cells were measured by qRT-PCR (mean+/- s.e.m., n=5)."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(I) Full length and a truncated version of HA-HuR without the hinge region (graphical scheme in upper panel) was used to score the effect of deletion of hinge region on EV associated miR-122 content measured by qRT-PCR (bottom panel). Western blots to check expression of HuR and its truncated variants are also shown.\" * \" denotes the HA-HuR band (left lower panel). Data represented as mean+/- s.e.m., n=3."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(J) Ubiquitination status of full length and a truncated version of HA-HuR without the hinge region between RRMII and RRMIII in Huh7 cells. HuR (full lenght or truncated version) were immunoprecipitated from cell lysates of Huh7 cells expressing these proteins and the immunoprecipitated materials were western blotted for HA-HuR and ubiquitination. Ubiquinitated HuR bands are marked by arrows. postion of non-ubiquitinated proteins were marked by arrowheads (upper panel). qRT-PCR based estimation of associated miRNA per unit of HA-HuR immunoprecipitated materials were estimated and normalized against respective protein levels quantified by densitometric quantification of western blots (lower panel). Data represented as mean+/- s.e.m., n=3. PCIneo transfected cells were used as control in immunoprecipitation reaction but no amplification of miR-122 were detected for HA immunoprecipitated materials from pCIneo tranfected cells."
NCBI gene IDs:
"HuR: 1994
miR-122: 406906"
Figure legend: "(A and B) qPCR analysis (n = 3) of BRCA1 mRNA (A) and pre-mRNA levels (B) in different cell cycle phases of HeLa cells."
NCBI gene IDs:
"BRCA1: 672"
Figure legend: "(C) ChIP analysis (n = 3) of Pol II binding to BRCA1 promoter during G1 and S phases in HeLa cells. (D) ChIP analysis (n = 3) of Pol II-pSer2 binding to BRCA1 3'UTR during G1 and S phases in HeLa cells. Pol II-pSer2, phosphorylation of RNA Pol II CTD Ser2."
NCBI gene IDs:
"BRCA1: 672"
Figure legend: "Analysis of BRCA1 WT or 5q isoform RNA levels after the depletion of UPF1(n = 3) (O) or BRCA1 exon5 alternative splicing (n = 3) (AS) during the indicated cell cycle phases (P) by semi-quantitative RT-PCR (sqRT-PCR). Left, bar plot showing the concentration of BRCA1 isoforms in exon5 AS (O) or the ratio of BRCA1 WT isoform in exon5/11 AS (P the above is the representative DNA gel. Right, signal figure showing quantitative analysis of PCR products. RFU, relative fluorescence units. (P) HeLa cells were transfected with UPF1 siRNA, then BRCA1 WT and 5q isoform mRNA levels during the indicated cell cycle phases were analyzed by qPCR (n = 3). siNC, transfected with control siRNA. siUPF1, transfected with UPF1 siRNA."
NCBI gene IDs:
"BRCA1: 672
UPF1: 5976"
Figure legend: "Analysis of BRCA1 WT or 5q isoform RNA levels after the depletion of UPF1(n = 3) during the indicated cell cycle phases by semi-quantitative RT-PCR (sqRT-PCR). siNC, transfected with control siRNA. siUPF1, transfected with UPF1 siRNA."
NCBI gene IDs:
"BRCA1: 672
UPF1: 5976"
Figure legend: "(C and D) RNA pull-down assay shows RBM10 binding to BRCA1 exon5 (C) and exon11 (D) RNA in vitro."
NCBI gene IDs:
"BRCA1: 672"
Figure legend: "(G and H) The BRCA1 protein (G) and mRNA levels (n = 3) (H) in 293T and RBM10-KD cells."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "(N and O) qPCR analysis (n = 3) of BRCA1 WT isoform mRNA level in exon11 AS during the indicated cell phases after depleting RBM10 in HeLa (N) and MCF-7 (O) cells."
NCBI gene IDs:
"BRCA1: 672
RBM10: 8241"
Figure legend: "(P) Results of western blot analyzing BRCA1 levels in HeLa and RBM10-KD cells in the indicated cell cycle phases."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "(A to D) Analysis of BRCA1 exon5 (A) and exon11 (C) AS by by semi-quantitative RT-PCR (sqRT-PCR) (n = 3), and analysis of BRCA1 exon5 (n = 3) (B) or exon11 (n = 3) (D) AS by minigene splicing reporter system after the depletion of RBM10. Bar plot shows the ratio of BRCA1 WT isoform in exon5/11 AS, the figure above is the representative DNA gel, the right is signal figure showing quantitative analysis of PCR products. RFU, relative fluorescence units."
NCBI gene IDs:
"BRCA1: 672
RBM10: 8241"
Figure legend: "(F) qPCR (n = 3) analyzing the WT and D11q isoform levels (exon11 AS) after RBM10 overexpression in 293T cells."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "(F) Analysis of BRCA1 exon5 AS by by sqRT-PCR (n = 3) in in G1/S phase of 293T cells treated with CDK2i. In (F) bar plot showing the ratio of BRCA1 WT isoform in exon5 AS, the above is the representative DNA gel. Right, signal figure showing quantitative analysis of PCR products. RFU, relative fluorescence units. Data are presented as mean ± s.d. in all qPCR."
NCBI gene IDs:
"BRCA1: 672"
Figure legend: "(N) Analysis of BRCA1 exon5 AS by sqRT-PCR (n = 3), 293T cell was transfected with RBM10 S89D variant in G1 phase. In (N), bar plot showing the ratio of BRCA1 WT isoform in exon5 AS, the above is the representative DNA gel. Right, signal figure showing quantitative analysis of PCR products. RFU, relative fluorescence units. Data are presented as mean ± s.d. in all qPCR."
NCBI gene IDs:
"BRCA1: 672
RBM10: 8241"
Figure legend: "(O to Q) Phosphorylation at S89 of the indicated RBM10 variants (O), WT RBM10 in the indicated cell cycle phases (P), and WT/S89A mutant RBM10 after CDK2i treatment in G1/S phase (Q) were analyzed in 293T cells."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "(D) qPCR revealed the fold change (UPF1 knockdown vs control) of candidate genes in (C) with G1 down-expression depending on NMD (n = 3), the region above the dashed line means that depleting UPF1 increases the mRNA level of the candidate genes . Data are presented as mean ± s.d. in (D) two-sided unpaired t-test is used to determine the differences."
NCBI gene IDs:
"UPF1: 5976"
Figure legend: "γH2AX levels in RBM10-KD #4 cells under the treatments as indicated. Representative images were shown in (A). CPT, camptothecin. BLM, bleomycin. IR, ionizing radiation."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "Immunofluorescence analysis of γH2AX in RBM10-KD #4 cells complemented with either RBM10."
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "(H and I) HeLa cells were transfected with RBM10 or/with 53BP1 siRNA. Immunofluorescence analysis of γH2AX after CPT (H) and cisplatin (I) treatment."
NCBI gene IDs:
"RBM10: 8241
53BP1: 7158"
Figure legend: "Colony formation assay (n = 3) was performed in RBM10-depleted 293T cells treated with ionizing radiation (n = 3) (K)"
NCBI gene IDs:
"RBM10: 8241"
Figure legend: "WB analysis (C) of the indicated genes or proteins in parental or IκBα KO HT29 cells obtained at pre-confluence or 7 days post-confluence. Bars represent mean values ± standard deviation of 3 technical replicates from 3 biological replicates performed."
NCBI gene IDs:
"IκBα: 4792"
Figure legend: "(H) Ifng protein and Ifng mRNA levels (n = 4/group) were determined by ELISA and quantitative PCR."
NCBI gene IDs:
"Ifng: 15978"
Figure legend: "(A) m6A dot blot assay using total RNA from NK92-siCTRL or NK92-siFTO cells."
NCBI gene IDs:
"FTO: 79068"
Figure legend: "(D) Western blotting for the detection of GZMB in NK92-siCTRL and NK92-siFTO cells after treatment with IL-2."
NCBI gene IDs:
"FTO: 79068"
Figure legend: "(B) Western blotting analysis. NK92-siCTRL or NK92-siFTO cells were incubated with 20 ng/ml IL-2 for 24 hours, washed, and cultured without IL-2 (IL-2 depletion) for the indicated times (n = 3). (C) A western blotting analysis of NK92+MOCK or NK92+FTO cells incubated with 20 ng/ml IL-2 for the indicated times was performed (n = 3). D"
NCBI gene IDs:
"FTO: 79068"