EMBO/SourceData huggingface dataset:
Figure legend: "Scatterplots showing correlations between CALIC- and hnRNP-L-regulated genes in (B). Left, co-regulated 59 genes; Right, all differentially regulated genes (DEGs). The correlation was calculated using the data except for the gene indicated by the asterisk."
NCBI gene IDs:
"CALIC: 
hnRNP-L: 3191"
Figure legend: "Genes induced by CALIC are significantly enriched in those upregulated by hnRNP-L. GSEA comparing the genes repressed by CALIC and hnRNP-L knockdown. NES, normalized enrichment score."
NCBI gene IDs:
"CALIC: 
hnRNP-L: 3191"
Figure legend: "Heatmap showing the expression levels of genes positively regulated in common by both CALIC and hnRNP-L in colon and rectal cancers. Samples are ordered based on the expression levels of AXL."
NCBI gene IDs:
"CALIC: 
AXL: 558
hnRNP-L: 3191"
Figure legend: "qRT-PCR analyses of AXL in HCT116 and LS180 cells transfected with an siRNAs targeting CALIC"
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "immunoblotting (B, D) analyses of AXL in HCT116 and LS180 cells transfected with an siRNAs targeting CALIC"
NCBI gene IDs:
"CALIC: "
Figure legend: "qRT-PCR analyses of AXL in HCT116 and LS180 cells transfected with an siRNAs targeting hnRNP-L."
NCBI gene IDs:
"AXL: 558
hnRNP-L: 3191"
Figure legend: "immunoblotting (B, D) analyses of AXL in HCT116 and LS180 cells transfected with an siRNAs targeting hnRNP-L."
NCBI gene IDs:
"hnRNP-L: 3191"
Figure legend: "qRT-PCR analyses of AXL in HCT116 cells infected with a lentivirus expressing wild-type CALIC (CALIC-full), CALIC-296-913 or CALIC-mut"
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "HCT116 cells that had been treated with an siRNA targeting hnRNP-L or CALIC were transfected with an AXL reporter construct and subjected to luciferase assays."
NCBI gene IDs:
"CALIC: 
AXL: 558
hnRNP-L: 3191"
Figure legend: "ChIP assays were performed using anti-hnRNP-L antibody or control mouse IgG. The promoter regions of GAPDH and Egr1 were used as negative and positive controls, respectively."
NCBI gene IDs:
"GAPDH: 
Egr1: 13653"
Figure legend: "ChIP analysis of hnRNP-L association with the AXL promoter region in CALIC knockdown HCT116 cells."
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "ChIP analysis of hnRNP-L association with the AXL promoter region in HCT116 cells expressing lentiviral CALIC-full."
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "ChIRP analysis of CALIC binding to the AXL promoter. Binding of CALIC to the promoter regions of the indicated genes was compared with that of LacZ."
NCBI gene IDs:
"AXL: 558"
Figure legend: "Migratory activity of AXL knockdown HCT116 cells."
NCBI gene IDs:
"AXL: 558"
Figure legend: "Lentiviral expression of AXL restores the migration activity of HCT116 cells transfected with an siRNA targeting CALIC. Immunoblotting and qRT-PCR analyses of HA-tagged AXL and CALIC expression, respectively (left). α-Tubulin was used as a loading control."
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "Representative images of gross specimens (top) and H&amp;E stained sections (bottom) of lung metastatic lesions in mice injected with CALIC or AXL knockdown HCT116 cells. Tumors were marked with yellow arrows. The dashed lines depict the boundary between normal and tumor tissues. (Right) Percentage of mice with lung metastases."
NCBI gene IDs:
"CALIC: 
AXL: 558"
Figure legend: "Representative images of gross specimens of primary cecal tumors (top) and liver metastatic lesions (bottom) in mice orthotopically (cecal) injected with CALIC knockdown HCT116 cells. Tumors are marked with yellow arrows. (Right) Percentage of mice with primary tumors and liver metastases."
NCBI gene IDs:
"CALIC: "
Figure legend: "A, B. A missense mutation in SPOP gene (Q165P, T &gt;G) was identified in a PCa patient. Exome sequencing revealed that the primary tumor contained a heterozygous Q165P mutation (A) whereas the liver metastasis harbored a homozygous Q165P mutation (B)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "D, E. IHC analysis of BRD4 protein expression in SPOP wild type (WT) and mutated (MUT) PCa patient samples. The representative images of BRD4 IHC staining in both SPOP WT and MUT PCa patients are shown in (D) and the quantified IHC data are shown in (E). Scale bars: 100 µm for 20 x fields; 50 µm for 40 x fields. The red dot in (E) indicates SPOP Q165P sample. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; ** P &lt; 0.01. See Appendix Table S4 for the detailed comparison, P values and sample number (n)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "F, G. IHC analysis of AR protein expression in SPOP WT and mutated PCa patient samples. The representative images of AR IHC staining in both SPOP WT and mutant PCa patients are shown in (F) and the quantified IHC data are shown in (G). Scale bars: 100 µm for 20 x fields; 50 µm for 40 x fields. The green dot indicates SPOP Q165P sample. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; *** P &lt; 0.01. See Appendix Table S4 for the detailed comparison, P values and sample number (n)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "C. C4-2 cells were stably infected with the lenti-virus expressing empty vector (EV) or SPOP mutants and harvested for western blot analysis with the indicated antibodies."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "D. C4-2 xenograft tumors expressing EV or F133V and SPOP WT and Q165P PDX tumors were harvested for western blot analysis with the indicated antibodies. T1: tumor 1; T2: tumor 2."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "E. Representative IHC images of PTEN protein in SPOP WT and Q165P PDX tumors. Scale bars: 100 µm for 20 x fields; 50 µm for 40 x fields."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Co-IP assay was performed to detect the efficiency of dimerization between SPOP WT and Q165P mutant (F)"
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Co-IP assay was performed to detect the interaction between CULLIN3 with SPOP WT or Q165P (G)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "A. DU145 PCa cells infected with virus expressing EV or SPOP Q165P were harvested for western blot analysis."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Clonogenic survival assay was performed to determine the sensitivity of NEO2734 in SPOP Q165P DU145 cells. The survival curve showed IC50 for SPOP Q165P (0.69 µM) and EV cells (1.08 µM) (C)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "DU145 cells expressing lenti-EV or lenti-HA-SPOP-Q165 constructs were treated with 1 µM of NEO2734 for 4 days and cultured for another 8 days before harvest. The number of colonies with more than 50 cells was counted. Representative images of colonies are shown in (D) with quantitative data shown in (E). All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; * P &lt; 0.05, ** P &lt; 0.01."
NCBI gene IDs:
"HA: 
SPOP: 8405"
Figure legend: "F. DU145 cells were treated with the indicated inhibitors and harvested for ChIP with BRD4 antibody. The enrichment of BRD4 at c-MYC enhancer and RAC1 promoter was analyzed using qPCR. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; n.s., not significant, * P &lt; 0.05, ** P &lt; 0.01."
NCBI gene IDs:
"BRD4: 23476
c-MYC: 4609
RAC1: 5879"
Figure legend: "G. DU145 cells were treated with the indicated inhibitors and harvested for ChIP with Ac-H3 antibody. The level of Ac-H3 at c-MYC enhancer and RAC1 promoter is analyzed using qPCR. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; ** P &lt; 0.01."
NCBI gene IDs:
"H3: 
c-MYC: 4609
RAC1: 5879"
Figure legend: "Six organoid lines including four SPOP WT (BM1, BM5, ST1 and 313HR) and two MUT (573R and ASC1) were harvested for western blot analysis with the indicated antibodies (A) Asterisk, SRC3 at expected molecular mass."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Six organoid lines including four SPOP WT (BM1, BM5, ST1 and 313HR) and two MUT (573R and ASC1) were harvested for IFC with p-AKT-S473 antibody (B). E-cadherin antibody was used to indicate the cell membrane (red) and DAPI for nucleus (blue). Scale bars: 25 µm."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Six organoid lines including four SPOP WT (BM1, BM5, ST1 and 313HR) and two MUT (573R and ASC1) were cultured for five days, followed by the treatment with JQ1 (2 µM), CPI-637 (2 µM), JQ1 (2 µM) + CPI-637 (2 µM) or NEO2734 (2 µM) for five more days. The representative images of organoids after the treatment are shown in (C)"
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "Six organoid lines including four SPOP WT (BM1, BM5, ST1 and 313HR) and two MUT (573R and ASC1) were cultured for five days, followed by the treatment with JQ1 (2 µM), CPI-637 (2 µM), JQ1 (2 µM) + CPI-637 (2 µM) or NEO2734 (2 µM) for five more days. the quantified data of the organoid diameter are shown in (D). Scale bars: 25 µm. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; n.s., not significant, * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "E, F. 313HR (SPOP WT) and 573R (Q165P) organoids were cultured for 5 days, followed by the treatment with the indicated inhibitors for 5 days. The organoids were stained with Caspase-3 antibody (green) to detect apoptotic cells. E-cadherin antibody staining was used to define the cell membrane (red) and the nucleus was counterstained with DAPI (blue). The representative images of cleaved Caspase-3 IFC staining are shown in (E). Scale bars: 25 µm. All data shown are means ± SEM. The P value was calculated by the unpaired two-tailed Student's t-test; * P &lt; 0.05, *** P &lt; 0.001."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "A. C4-2 PCa cells infected with virus expressing EV, SPOP Q165P or F133V were harvested for western blot analysis."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "C. Clonogenic survival assay was performed to determine the sensitivity of NEO2734 in SPOP mutant C4-2 cells. The survival curve showed IC50 for EV (1.01 µM), SPOP Q165P (0.621 µM) and SPOP F133V cells (0.395 µM)."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "F - H. A schematic depicts the procedure of the establishment of SPOP mutant xenograft models and inhibitor administration (F). When the tumor reached 100 mm3, mice were treated with vehicle (40% polyethylene glycol), JQ1 (50 mg/kg) or NEO2734 (30 mg/kg) five days a week for three consecutive weeks. Tumors isolated from mice at day 21 of treatment were photographed (G) and tumor growth are shown in (H). All data shown are means ± SEM. The P value comparing the tumor volume at day 21 post- treatment was calculated by the unpaired two-tailed Student's t-test; * P &lt; 0.05, ** P &lt; 0.01; *** P &lt; 0.001."
NCBI gene IDs:
"SPOP: 8405"
Figure legend: "HEK293A cells were transfected with expression plasmids for Siglec‐7, Siglec‐5 or Siglec‐14, and then subsequently incubated with (red) or without (gray) recombinant Hsp70 (bottom panels). Flow cytometry analysis reveals Hsp70 binding to cell surfaces of HEK293A cells expressing Siglec‐5 and Siglec‐14, but not Siglec‐7. Histogram of Siglec expression after transfection is shown as blue lines (top panels)."
NCBI gene IDs:
"Siglec‐14: 100049587
Siglec‐5: 8778
Siglec‐7: 27036"
Figure legend: "Using flow cytometry, undifferentiated or PMA‐differentiated THP1 cells with empty vector (EV), Siglec‐5 (Sig5), or Siglec‐14 (Sig14) overexpression were assessed for the presence of endogenously secreted Hsp70 bound on the cell surface. The mean fluorescence intensity (MFI) of signals from an anti‐Hsp70 antibody on unpermeabilized THP1 cells is shown."
NCBI gene IDs:
"Siglec‐14: 100049587
Siglec‐5: 8778"
Figure legend: "Secretion of pro‐inflammatory cytokine TNFα by THP1 cells transfected with an empty vector (EV) or Siglec‐5 (Sig5) expression plasmid was evaluated by ELISA after concurrent exposure with LPS and 10 μg/ml Hsp70 or LPS alone. Secretion of TNFα was reduced only in THP1 Sig5 cells exposed to both LPS and Hsp70 but not LPS alone."
NCBI gene IDs:
"Sig5: 8778
Siglec‐5: 8778"
Figure legend: "Similarly, IL‐8 secretion from THP1 EV and Sig5 cells was evaluated by ELISA after concurrent exposure with LPS and 10 μg/ml Hsp70. Secretion of IL‐8 was also reduced in only THP1 Sig5cells exposed to both LPS and Hsp70 but not LPS alone."
NCBI gene IDs:
"Sig5: 8778"
Figure legend: "THP1 Sig5 cells were incubated with Hsp70, and the levels of SHP‐1 recruitment to Siglec‐5 were evaluated by immunoprecipitation of Siglec‐5 and Western blot for SHP‐1. Higher levels of SHP‐1 were co‐immunoprecipitated with Siglec‐5 when the cells were incubated with Hsp70."
NCBI gene IDs:
"Sig5: 8778"
Figure legend: "Western blots demonstrate phosphorylated p38 (p‐p38) was reduced in THP1Siglec‐5cells when exposed to both LPS and Hsp70 in comparison with LPS alone. This reduction was also greater in THP1Siglec‐5cells in comparison with the control THP1cells. Numbers below immunoblots indicate densitometric analysis of each band normalized to the respective cell line's unstimulated control group, divided by the respective loading control (total p38)."
NCBI gene IDs:
"Siglec‐5: 8778"
Figure legend: "Secretion of pro‐inflammatory cytokine IL‐8 by PMA‐differentiated THP1 EV or THP Sig5 cells was evaluated by ELISA after concurrent exposure with 10 ng/ml LPS with an anti‐Hsp70 antibody or isotype‐matched antibody. Secretion of IL‐8 was increased in only THP1 Sig5 cells exposed to LPS and an anti‐Hsp70 antibody in comparison with the isotype control antibody, but this increase was not replicated in THP1 EV cells."
NCBI gene IDs:
"Sig5: 8778"
Figure legend: "Secretion of TNFα by THP1 cells transfected with a Siglec‐5 or Siglec‐14 expression vector was evaluated by ELISA after exposure to Hsp70 at the various concentrations in the presence of endotoxin‐neutralizing agent polymyxin B. Only THP1 Siglec‐14 cells exhibited significant secretion of TNFα in an Hsp70 dose‐dependent manner."
NCBI gene IDs:
"Siglec‐14: 100049587
Siglec‐5: 8778"
Figure legend: "IL‐8 secretion was also evaluated after THP1Siglec‐5 and Siglec‐14cells were exposed to Hsp70. Similarly, only THP1Siglec‐14cells exhibited significant secretion of IL‐8 in an Hsp70 dose‐dependent manner."
NCBI gene IDs:
"Siglec‐14: 100049587
Siglec‐5: 8778"
Figure legend: "Western blot analysis demonstrates a greater increase in phosphorylated p38 (p‐p38) from THP1 Siglec‐14 cells after exposure to Hsp70 in comparison with control THP1 EV cells in a time‐dependent manner. Graph below immunoblots indicates densitometric analysis of each band normalized to the respective cell line's unstimulated control group, divided by the respective loading control."
NCBI gene IDs:
"Siglec‐14: 100049587"
Figure legend: "Exposure to Hsp70 also yielded greater degradation of the NF‐κB inhibitor, IκBα, in THP1 Siglec‐14 cells in comparison with control THP1 cells, augmentation of the pro‐inflammatory response. Graph below immunoblots indicates densitometric analysis of each band normalized to the respective cell line's unstimulated control group, divided by the respective loading control."
NCBI gene IDs:
"Siglec‐14: 100049587"
Figure legend: "Secretion of pro‐inflammatory cytokine IL‐8 by PMA‐differentiated THP1 EV or THP Sig 14 cells was evaluated by ELISA after concurrent exposure with an anti‐Hsp70 antibody or isotype‐matched antibody. Secretion of IL‐8 was decreased in only THP1 Sig 14 cells exposed to LPS and an anti‐Hsp70 antibody in comparison with the isotype control antibody, but this change was not observed in THP1 EV cells."
NCBI gene IDs:
"Sig 14: 100049587"
Figure legend: "TNFα secretion from SIGLEC14+/+ monocytes was measured after exposure to LPS or Hsp70 in the presence or absence of endotoxin‐neutralizing compound polymyxin B. Hsp70 was able to induce secretion of TNFα even in the presence of polymyxin B, suggesting a mechanism independent of endotoxin contaminants. DnaK, the E. coli homolog of human Hsp70, did not induce significant secretion of TNFα above background levels."
NCBI gene IDs:
"SIGLEC14: 100049587"
Figure legend: "TNFα secretion from SIGLEC14+/+ monocytes by Hsp70 was reduced when the cells were exposed to an anti‐Siglec‐5/Siglec‐14 blocking antibody."
NCBI gene IDs:
"SIGLEC14: 100049587"
Figure legend: "Human monocytes isolated from SIGLEC14+/+, SIGLEC14+/−, and SIGLEC14−/− individuals were exposed to varying concentrations of Hsp70, and TNFα secretion was evaluated. The amount of TNFα secreted was normalized to the respective cells' response to 1 ng/ml LPS to minimize the impact of other possible polymorphisms and factors that contribute to variation between individuals."
NCBI gene IDs:
"SIGLEC14: 100049587"
Figure legend: "TNFα secretion from SIGLEC14−/− monocytes stimulated with 15 ng/ml LPS and various concentrations of Hsp70. TNFα secretion was reduced with increasing concentrations of Hsp70."
NCBI gene IDs:
"SIGLEC14: 100049587"
Figure legend: "A HERC3 increases the protein levels of active YAP, p-YAP (S381), YAP and TAZ. active-YAP, p-YAP (S381), p-YAP (S127), YAP, p-TAZ (S89), TAZ, HERC3 and β-Actin were detected by Western blotting with appropriate antibodies as indicated in MDA-MB-231 cells stably expressing HERC3-WT or HERC3-CA. Ctrl, Control; WT, wildtype of HERC3; CA, HERC3-CA, a catalytically inactive mutant of HERC3."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "B HERC3 reverses the reduction in the active YAP and TAZ levels induced by serum starvation. MDA-MB-231 cells stably expressing HERC3 or HERC3-CA were cultured in medium with or without fetal bovine serum (FBS) for 1 h. Cells were harvested for Western blotting analysis with appropriate antibodies as indicated. Ctrl, Control; WT, wildtype of HERC3; CA, HERC3-CA, a catalytically inactive mutant of HERC3."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "C Depletion of HERC3 decreases the steady-state levels of YAP/TAZ. Levels of active-YAP, p-YAP (S381), p-YAP (S127), YAP, p-TAZ (S89), TAZ, HERC3 and β-Actin were detected by Western blotting with appropriate antibodies as indicated in MDA-MB-231 and SUM159 cells stably expressing shCtrl (Control shRNA), sh-1 and sh-2 (two shRNAs against HERC3)."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "E Depletion of HERC3 has no effects on mRNA levels of YAP/TAZ. Total mRNA levels of YAP/TAZ in control or HERC3-deficient MDA-MB-231 cells were analyzed by qRT-PCR using primers specific to YAP/TAZ. Data are shown as mean ±SEM; n = 3 biological replicates. Statistical analysis was performed using two-tailed Student t test. n.s., not significant."
NCBI gene IDs:
"HERC3: 8916
TAZ: 6901
YAP: 10413"
Figure legend: "G Depletion of HERC3 attenuates YAP- or TAZ-induced 8xGTIIC-Luc reporter activity. HEK293T cells were transfected with expression plasmids for YAP or TAZ, shHERC3 or shCtrl, and reporter plasmids 8xGTIIC-Luc and Renilla-Luc as indicated. Relative luciferase activity was measured as described in the text. Western blots showing expression of indicated proteins were shown. H Depletion of HERC3 attenuates YAP- or TAZ-induced CTGF-Luc reporter activity. Cell transfection and luciferase assay were carried out as described in Panel G. Western blots showing expression of indicated proteins were shown. Data are shown as mean ±SEM; n = 3 biological replicates. Statistical analysis was performed using two-tailed Student t test. **P &lt; 0.01."
NCBI gene IDs:
"Luc: 
HERC3: 8916
TAZ: 6901
YAP: 10413"
Figure legend: "I Depletion of HERC3 attenuates transcription of YAP/TAZ target genes. Total mRNA levels of CTGF and ANKRD1 in control or HERC3-deficient MDA-MB-231 cells were analyzed by qRT-PCR using primers specific to the indicated target gene. Data are shown as mean ±SEM; n = 3 biological replicates. Statistical analysis was performed using two-tailed Student t test. ***P &lt; 0.001."
NCBI gene IDs:
"ANKRD1: 27063
CTGF: 1490
HERC3: 8916"
Figure legend: "A HERC3 and HERC3-CA block β-TrCP-mediated degradation of TAZ. HEK293T cells were transfected with expression plasmids for HA-β-TrCP, Myc-HERC3 or Myc-HERC3-CA, FLAG-TAZ or FLAG-GFP as indicated. Cells were harvested 24 h post-transfection. Protein levels were detected by Western blotting with appropriate antibodies as indicated."
NCBI gene IDs:
"FLAG: 
GFP: 
HA: 
Myc: 
β-TrCP: 8945
HERC3: 8916
TAZ: 6901"
Figure legend: "D HERC3 and HERC3-CA extend the half-life of TAZ. Control or HERC3-deficient MDA-MB-231 cells were treated with cycloheximide (CHX, 20 µM) for 0, 4, 8 or 12 h before harvested. Left, Western blotting with appropriate antibodies. Right, Image J quantitation of YAP intensity and normalized to β-Actin at the indicated time points (n = 3 biological replicates). Data are represented as the mean ± SEM (right panel)."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "F Depletion of HERC3 decreases the half-life of YAP/TAZ. Control or HERC3-deficient MDA-MB-231 cells were treated with cycloheximide (CHX, 20 µM) for 0, 2, 4 or 6 h before harvested. Left, Western blotting with appropriate antibodies. Right, Image J quantitation of YAP intensity and normalized to β-Actin at the indicated time points (n = 3 biological replicates). Data are represented as the mean ± SEM (right panel)."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "G Depletion of β-TrCP reverses shHERC3-induced degradation of YAP/TAZ. Control or HERC3-deficient MDA-MB-231 were transfected with siCtrl or siβ-TrCP as indicated. Cells were harvested 48 h post-transfection. Protein levels were detected by Western blotting with appropriate antibodies as indicated."
NCBI gene IDs:
"β-TrCP: 8945
HERC3: 8916"
Figure legend: "A HERC3 interacts with β-TrCP, but not YAP or TAZ. HEK293T cells were transfected with expression plasmids for FLAG-HERC3, HA-YAP or HA-TAZ or HA-β-TrCP. Cells were harvested 24 h post-transfection and subjected to IP with FLAG antibody-conjugated agarose beads. Protein levels were detected by Western blotting with HA and FLAG antibodies. B HERC3 interacts with endogenous β-TrCP. MDA-MB-231 cells stably expressing HERC3 or HERC3-CA were harvested and subjected to IP with IgG or HA antibodies. Protein levels were detected by Western blotting with β-TRCP, HA and β-Actin antibodies. C"
NCBI gene IDs:
"FLAG: 
HA: 
β-TrCP: 8945
HERC3: 8916
TAZ: 6901
YAP: 10413"
Figure legend: "H HERC3 blocks the interaction between β-TrCP and YAP. HEK293T cells were transfected with expression plasmids for FLAG-YAP, HA-β-TRCP, Myc-HERC3 and Myc-HERC3-CA as indicated. Cells were harvested 24 h post-transfection and subjected to IP with FLAG antibody-conjugated agarose beads. Protein levels were detected by Western blotting with HA, FLAG and Myc antibodies."
NCBI gene IDs:
"FLAG: 
HA: 
Myc: 
β-TRCP: 8945
HERC3: 8916
YAP: 10413"
Figure legend: "A Depletion of HERC3 reduces cell proliferation. Control or HERC3-deficient MDA-MB-231 were analyzed for indicated days by CCK8 assay as described in the materials and methods. Data are shown as mean ± SEM; n = 3 biological replicates."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "C Depletion of HERC3 inhibits colony formation in MDA-MB-231 cells. Control or HERC3-deficient MDA-MB-231 were analyzed by colony formation assay. Quantification of the results in colony formation assays was analyzed by Image J. D Depletion of HERC3 inhibits colony formation in SUM159 cells. Control or HERC3-deficient SUM159 were analyzed by colony formation assay. Quantification of the results in colony formation assays was analyzed by Image J."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "Depletion of HERC3 attenuates cell migration. Control or HERC3-deficient MDA-MB-231 cells (upper panel) and SUM159 cells (lower panel) were analyzed by transwell migration assay. E, migrated were fixed in 4% PFA and stained with 0.1% crystal violet and photographed. data quantification of migrated MDA-MB-231 cells (left panel) and SUM159 (right panel) cells counts by Image J."
NCBI gene IDs:
"HERC3: 8916"
Figure legend: "H RNAi-resistant HERC3 and YAP/TAZ mutants reverse the inhibitory effect of HERC3 depletion on colony formation. Ectopic expression of RNAi-resistant HERC3-WT, HERC3-CA and YAP/TAZ mutants in HERC3-deficient MDA-MB-231 cells were subjected to colony formation assay as described in Panel C."
NCBI gene IDs:
"HERC3: 8916
TAZ: 6901
YAP: 10413"