EMBO/SourceData huggingface dataset:
Figure legend: "Immunoprecipitation analysis of HEK293T cells expressing Flag-MAVS or 4KR mutant together with Myc-Ub and HA-RNF34. Anti-Flag immunoprecipitates were analyzed using immunoblotting with an anti-Myc or anti-Ub-K27 antibody. Levels of the transfected proteins were analyzed by performing immunoblotting with an anti-Flag antibody. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
Ub: 
MAVS: 57506
RNF34: 80196"
Figure legend: "(C) Immunoprecipitation analysis of HEK293T cells expressing Flag-MAVS or K311R mutant together with HA-RNF34 and/or Myc-TRIM31 following a 3MA (0.2 mM) treatment. Anti-Flag immunoprecipitates were analyzed using immunoblotting with an anti-Flag, anti-Ub-K63 or anti-Ub-K27 antibody. Levels of the transfected proteins were analyzed using immunoblotting with an anti-HA or anti-Flag antibody. ell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
MAVS: 57506
RNF34: 80196
TRIM31: 11074"
Figure legend: "(D) Co-immunoprecipitation analysis of the ubiquitination of endogenous MAVS in THP-1 cells transfected with siCtrl, siRNF34, or siTRIM31 oligos and infected with VSV for the indicated times. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196
TRIM31: 11074"
Figure legend: "(C) Immunoblot showing the levels of the MAVS protein in HEK293T cells expressing Flag-MAVS or Flag-mMAVS together with Myc-RNF34. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
Myc: 
MAVS: 57506
mMAVS: 57506
RNF34: 80196"
Figure legend: "(D) Immunoblot showing the levels of the MAVS protein in HEK293T cells expressing Flag-MAVS or its 4KR mutant together with increasing amounts of Myc-RNF34. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
Myc: 
MAVS: 57506
RNF34: 80196"
Figure legend: "(E) Immunoblot showing the levels of the MAVS protein in HEK293T cells expressing Flag-MAVS with increasing amounts of HA-RNF34 or its H342A mutant. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
MAVS: 57506
RNF34: 80196"
Figure legend: "(F) SDD-AGE analysis of MAVS aggregates in HEK293T cells expressing Flag-MAVS together with WT Myc-RNF34 or its ligase-dead mutant and cultured in the presence or absence of MG132 (10 μM), 3-MA (1 mM) or NH4Cl (3 mM). SDS-PAGE immunoblotting was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
Myc: 
MAVS: 57506
RNF34: 80196"
Figure legend: "(G) SDD-AGE analysis of MAVS aggregates in siCtrl- or siRNF34-1-transfected cells using an anti-MAVS antibody. SDS-PAGE immunoblotting was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "(H) Immunoblot showing the levels of the MAVS protein in siCtrl- or siRNF34-1-transfected PBMCs. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "(I) SDD-AGE analysis of MAVS aggregates in THP-1 cells expressing Flag-MAVS or the K311R mutant together with Myc-RNF34 or Myc-TRIM31. SDS-PAGE immunoblotting was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
Myc: 
MAVS: 57506
RNF34: 80196
TRIM31: 11074"
Figure legend: "(A) Immunoprecipitation analysis of HEK293T cells transfected with Flag-SQSTM1 or its I431A mutant together with HA-MAVS. Anti-Flag or IgG agarose immunoprecipitates were analyzed using immunoblotting with an anti-HA or anti-Flag antibody. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
MAVS: 57506
SQSTM1: 8878"
Figure legend: "(B) Luciferase activity driven by the IFN-β promoter in HEK293T cells transfected with HA-MAVS or its 4KR mutant together with Flag-SQSTM1 or its I431A mutant. Luciferase assays were performed 24 hrs after transfection. The luciferase reporter data are presented as means ± SEM. Two-tailed Student's t-tests were used for statistical analyses: *, P &lt; 0.05; **, P &lt; 0.01; and ***, P &lt; 0.001."
NCBI gene IDs:
"Flag: 
HA: 
luciferase: 
IFN-β: 3456
MAVS: 57506
SQSTM1: 8878"
Figure legend: "(C) Immunoblot showing the levels of the MAVS protein in HEK293T cells expressing HA-MAVS or its 4KR mutant together with Flag-SQSTM1. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
MAVS: 57506
SQSTM1: 8878"
Figure legend: "(D) Luciferase activity driven by the IFN-β promoter in HEK293T cells transfected with HA-MAVS or its 4KR mutant together with Flag-NDP52 or its D439R/C443K mutant. Luciferase assays were performed 24 hrs after transfection. The luciferase reporter data are presented as means ± SEM. Two-tailed Student's t-tests were used for statistical analyses: *, P &lt; 0.05; **, P &lt; 0.01; and ***, P &lt; 0.001."
NCBI gene IDs:
"Flag: 
HA: 
luciferase: 
NDP52: 10241
IFN-β: 3456
MAVS: 57506"
Figure legend: "(E) Immunoprecipitation analysis of HEK293T cells transfected with Flag-MAVS or its 4KR mutant together with Myc-NDP52. Anti-Flag immunoprecipitates were analyzed using immunoblotting with an anti-Myc or anti-Flag antibody. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
Myc: 
NDP52: 10241
MAVS: 57506"
Figure legend: "(G) Immunoprecipitation analysis of the interaction between Flag-NDP52 and HA-RNF34 in WT or MAVS-/- cells. Anti-Flag immunoprecipitates were analyzed using immunoblotting with an anti-HA antibody. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"MAVS: 57506"
Figure legend: "(H) Immunoprecipitation analysis of HEK293T cells transfected with Flag-NDP52 or its D439R/C443K mutant together with HA-MAVS followed by VSV infection. Anti-Flag immunoprecipitates were analyzed using immunoblotting with an anti-HA or anti-Flag antibody. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
HA: 
NDP52: 10241
MAVS: 57506"
Figure legend: "(I) Immunoblot showing the levels of the MAVS protein in siCtrl- or siNDP52-transfected cells expressing Flag-RNF34 or Flag-V. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
NDP52: 10241
RNF34: 80196"
Figure legend: "(J) Immunoblot showing the levels of the MAVS protein in siCtrl- or siNDP52-transfected cells infected with VSV (MOI = 1, 12 hrs). α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(K) Immunoprecipitation analysis of the MAVS-LC3 interaction in siCtrl- or siNDP52-transfected cells infected with VSV. Anti-MAVS immunoprecipitates were analyzed using immunoblotting with an anti-MAVS or anti-LC3 antibody. NDP52 levels were analyzed by performing immunoblotting with an anti-NDP52 antibody. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "(A) Immunoblot showing MAVS, IRF3-p, LC3, TOM20 and HSP60 levels in siCtrl- or siRNF34-1 cells infected with VSV and cultured in the presence of Z-VAD (50 μM) for the indicated times. α-Tubulin was used as a loading control. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "(B) Immunoblot showing MAVS, LC3, TOM20 and HSP60 levels in cells transfected with Flag-RNF34 or its H342A mutant and subsequently infected with VSV for the indicated times. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"Flag: 
RNF34: 80196"
Figure legend: "Representative confocal images (C) of immunofluorescence staining for NDP52 colocalization with MAVS in shCtrl- or shRNF34-1 cells infected with VSV for the indicated times. Red represents MAVS and green represents NDP52. Scale bar, 10 μm. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "quantification (D) of immunofluorescence staining for NDP52 colocalization with MAVS in shCtrl- or shRNF34-1 cells infected with VSV for the indicated times. Scale bar, 10 μm. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "(E) Transmission electron microscopy images of shCtrl- and shRNF34-1 HEK293T cells cultured in the presence or absence of VSV. Arrows indicate mitochondria engulfed in autophagosomes. Double arrows indicate swollen mitochondria with disrupted cristae. M, mitochondria and N, nucleus. 40000× magnification. Cell-based studies were performed independently at least three times with comparable results."
NCBI gene IDs:
"RNF34: 80196"
Figure legend: "(G) (left) Western blot of pERK in TNERT, TNERTFgfr1-/-, TNERTFgfr2-/-, TNERTFgf4-/-, and TNERTFgfbp1-/- with or no OHT. (Right) relative pERK levels estimated by densitometry  (n=4). Data information: n ≥ 3 biological replicates (each dot represents a biological replicate). Data are presented as mean ± SEM in G, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, and ns = not significant (paired two-tailed student's t-test)."
NCBI gene IDs:
"Fgf4: 14175
Fgfbp1: 14181
Fgfr1: 14182
Fgfr2: 14183"
Figure legend: "(C) Western blot of pERK and ERK in TNERT  and  TNERTNono-/- cells treated with or no OHT  (n=3)."
NCBI gene IDs:
"Nono: 53610"
Figure legend: "(D) Immunoprecipitation  analysis showing interactions between ERK and NONO  in the presence or absence of Nanog  induction by Doxycycline  in TDiN  cells."
NCBI gene IDs:
"Nanog: 71950"
Figure legend: "(E) 293T cells were infected with ULK1 shRNA or control shRNA lentiviruses and subjected to immunoprecipitation with control rabbit IgG or anti-AMPKα antibody followed by immunoblotting with anti-ULK1 and anti-AMPKα antibodies."
NCBI gene IDs:
"ULK1: 8408"
Figure legend: "(B) U-2OS cells stably expressing GFP-LC3 were infected with lentivirus expressing human Ulk1 shRNA (shhUlk1) or scrambled shRNA (shScr) and subjected to selection with 1 µg/ml puromycin for 5 days. The cells were then transfected with wild type mouse Ulk1 (mUlk1), mUlk1 Δ654-828 or control empty vector for 24 h, incubated in serum-free DMEM overnight, and cultured in complete medium with or without 10 mM metformin for 20 h. The images were obtained using a fluorescence microscope. The scale bars represent 10 µm."
NCBI gene IDs:
"Ulk1: 8408
Ulk1: 22241"
Figure legend: "(A) TSC2−/−, p53−/− MEFs stably reconstituted with wild-type raptor or S722A/S792A mutant raptor were transfected with GFP-LC3, treated with 2 mM AICAR or control vehicle for 4 h, and then analyzed by fluorescent microscopy. The number of GFP-LC3 dots per GFP-positive cell was counted (mean ± s.d.; n = 60). The scale bars represent 10 µm."
NCBI gene IDs:
"p53: 22059
TSC2: 22084"
Figure legend: "(B) TSC2−/−, p53−/− MEFs stably reconstituted with wild-type raptor or S722A/S792A mutant raptor were treated with 2 mM AICAR for the indicated times. Cell lysates were prepared in RIPA buffer and analyzed by immunoblotting with the indicated antibodies. The levels of p62 are listed relative to those of untreated WT raptor cells, which were set as 1."
NCBI gene IDs:
"p53: 22059
TSC2: 22084"
Figure legend: "E. Quantification of the nociceptive responses within 5 min after intraplantar injection of control saline in WT mice or 500 ppm iodine in WT and TRPA1-/-mice."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "F. Quantification of the nociceptive responses in TRPA1-/- mice within 5 min after intraplantar injection of control saline or 1,000 ppm iodine, following intraperitoneal injection of saline or vehicle."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "A. Left, time course of ear swelling elicited by topical application of 5% PVP-I solution in 0.15% Oxa-challenged WT and TRPA1-/-mice, or 5% povidone solution in Oxa-challenged WT mice. In this and subsequent similar figures, the number of mice is indicated. Right, bar graph highlighting the response at 24 h shown at left."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "C. Time course of ear swelling elicited by topical application of 3% iodine in 0.15% Oxa-challenged WT and TRPA1-/-mice, and bar graph highlighting the response at 24 h."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "D. Hematoxylin and eosin stained tissue sections of iodine treated ear in 0.15% Oxa-challenged WT and TRPA1-/-mice (n ≥ 3)."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "E. Time course of ear swelling elicited by topical application of 3% iodine in 0.15% Oxa-challenged TRPA1-/-mice, following intraperitoneal injection of AMG517 or vehicle, and bar graph highlighting the response at 24 h."
NCBI gene IDs:
"TRPA1: 277328"
Figure legend: "A. Representative intracellular Ca2+ signals in hTRPA1-expressing HEK293 cells in response to different concentrations of iodine. 2-APB, a TRPA1 agonist, was subsequently applied to fully activate TRPA1. RFU: relative fluorescence unit."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "B. Concentration-response relationships of iodine-induced intracellular Ca2+ increase in HEK293 cells expressing WT or mutant TRPA1 channels. Data are presented as mean ± s.e.m. n ≥ 8 for each construct at each concentration. The smooth curves are fits to the Hill equation."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "C. Time course of iodine-induced currents in hTRPA1-expressing Xenopus oocytes. HC: HC030031"
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "D. Concentration-response relationship of iodine-induced currents in hTRPA1-expressing Xenopus oocytes. Data are presented as mean ± s.e.m. n ≥ 8 for each concentration. The smooth curve is a fit to the Hill equation."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "E. Time course of intracellular iodine-induced macroscopic currents in an inside-out patch from hTRPA1-expressing HEK293 cell (n = 3)."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "F. Time course of iodine-induced currents in a hTRPA1-expressing Xenopus oocyte, which presents the current reduction upon DTT treatment (n = 3)."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "G, H. Comparisons of the iodine-induced whole-cell current in HEK293 cells expressing WT hTRPA1 (G) or the triple mutant channel (C421S/K710R/C856S) (H) (n ≥ 4)."
NCBI gene IDs:
"TRPA1: 8989"
Figure legend: "I. Representative whole-cell currents in HEK293 cells expressing human TRPV1 in response to iodine and subsequently applied capsaicin (n = 3)."
NCBI gene IDs:
"TRPV1: 7442"
Figure legend: "HO-1 expression on mRNA level quantified by qPCR qPCR analysis based on two independent experiments n = 10-11/group"
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "HO-1 expression on mRNA level quantified by RNA-seq. RNAseq analysis has n = 3-4/group, two-tailed t-test for two groups comparison, and one way ANOVA with Bonferroni post-test for multiple group comparison. *p&lt; 0.05, ****p&lt; 0.0001. Data are shown as mean ± SEM."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Middle-aged animals have lower frequency of ECs. Two independent experiments, n = 10-11/group. Data are shown as mean ± SEM. **p&lt;0.01, two-tailed unpaired t-test. The control staining of HO-1 on HO-1-/- bone marrow section is provided in Appendix Fig S1."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "RNA-seq revealed 111 DEGs in HO-1-/- ECs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "The identified DEGs separated HO-1-/- and HO-1+/+ ECs by (B) hierarchical clustering and (C) PCA."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Heatmap of selected (E) hematopoietic factors and (F) adhesion molecules expression in HO-1-/- and HO-1+/+ ECs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "RNA-seq revealed 100 DEGs in HO-1-/- CARs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "The identified DEGs separated HO-1-/- and HO-1+/+ CARs by (H) hierarchical clustering and (I) PCA."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Heatmap of selected (K) skeletal biology and (L) hematopoietic factors expression in HO-1-/- and HO-1+/+ CARs. Correlation similarity metric and average linkage clustering were used in the presented heatmaps."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "HO-1-/- mice have more ECs and less CARs (p = 0.053) in BM, while frequency of PaS does not differ."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Young HO-1-/- mice have lower frequency of Sca-1high fraction among BM ECs, what resembles phenotype of BM ECs in old mice."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Intracellular flow cytometry reveals (C) lower levels of SDF-1α in HO-1-/- CARs and (D) lower levels of LAP protein in HO-1-/- ECs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Analysis of cell surface SCF revealed lower frequency of SCF+ ECs in HO-1-/- while no differences among CARs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "The frequency of (F) CD163+ MQs and (G) CD163- MQs in BM of HO-1-/- mice is altered."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Young HO-1-/- mice possess higher number of (B) LT-HSCs, (C) ST-HSCs and (D) MPPs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "More young HO-1-/- LT-HSCs are in G1 and S/G2/M cell cycle phases. The presented cell cycle analysis is from two independent experiments. Young HO-1-/- MPPs do not differ in cell cycling from young HO-1+/+ MPPs. The presented cell cycle analysis is from two independent experiments. More old HO-1-/- LT-HSCs are in G1 phase in comparison to old HO-1+/+ LT-HSCs, but not in S/G2/M phase. The presented cell cycle analysis is from two independent experiments. Old MPPs do not differ in cell cycling between genotypes. The presented cell cycle analysis is from two independent experiments."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Young HO-1-/- LT-HSCs contain more γH2aXhigh cells than young HO-1+/+, 378-991 cells analyzed from 7 mice/group. Young HO-1-/- MPPs contain more γH2aXhigh cells than young HO-1+/+, 1693-2790 cells analyzed from 7 mice/group."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Alkaline comet assay revealed that HO-1-/- LT-HSCs possess (L) higher oil tail moment and (M) more DNA in the comet tail. 1068-1189 cells analyzed from 6 mice/group."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "HSCs from young HO-1-/- mice provide worse hematopoietic reconstitution after transplantation than HO-1+/+ HSCs. Data are shown as mean ± SEM, n = 8-9 mice/group."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "RNA-seq revealed 1067 DEGs between young HO-1-/- and HO-1+/+ LT-HSCs. RNA-seq revealed 595 DEGs between old HO-1-/- and HO-1+/+ LT-HSCs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "PC1 in PCA analysis on selected 1148 genes separated young HO-1+/+ LT-HSCs from other groups, clustering the young HO-1-/- LT-HSCs together with old LT-HSCs."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Hierarchical clustering on the 20% of most correlated 297 genes with PC1 showed 2 major clusters that includes young HO-1+/+ LT-HSCs in one and old LT-HSCs with young HO-1-/- LT-HSCs in second."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Deletion of HO-1 in myeloid lineage did not cause expansion of LT-HSCs and did not alter their cell cycle status."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Long-term PB chimerism derived from HSCs transplanted to HO-1-/- recipients is lower than from HSCs transplanted to HO-1+/+ recipients."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Chimerism among BM HSPCs fractions transplanted to HO-1-/- recipients are lower than from HSCs transplanted to HO-1+/+ recipients. Data are shown as mean ± SEM, n = 5-12/group"
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "total number of cells derived from HSCs transplanted to HO-1-/- recipients are lower than from HSCs transplanted to HO-1+/+ recipients. Data are shown as mean ± SEM, n = 5-12/group"
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Short term homing of HSPC to HO-1+/+ or HO-1-/- BM did not differ. Data are shown as mean ± SEM, n = 5/group, unpaired t-test."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Transplantation of HO-1-/- HSCs provides lower chimerism among HSPC fractions in primary recipients. Data are shown as mean ± SEM, n = 7-8 mice/group."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Comparison of gene log fold changes in non-transplanted HO-1-/- LT-HSCs and HO-1-/- LT-HSCs transplanted twice to the wild-type HO-1+/+ showed that transplantation of HO-1-/- LT-HSCs twice to the wild type niche reverses their transcriptional alterations."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Frequency of colonies appearing at day 8 and day 12 from HSCs co-cultured with HO-1+/+ or HO-1-/- MSCs. *p&lt; 0.05, ***p&lt;0.001, Fisher exact test."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Analysis of frequency of colonies derived from HO-1+/+ or from HO-1-/- MSC co-cultures among identified clusters. Enrichment in cluster analyzed by Fisher exact test, **p&lt;0.01, two-tailed unpaired t-test."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "Sdf1α mRNA levels in BM of HO-1+/+ or HO-1-/- mice after administration of Noggin. Data are shown as mean ± SEM. *p&lt; 0.05, two-tailed unpaired t-test, n = 10/group"
NCBI gene IDs:
"Sdf1α: 20315
HO-1: 15368"
Figure legend: "LT-HSC frequency of LT-HSCs after administration of Noggin to HO-1+/+ or HO-1-/- mice. Data are shown as mean ± SEM. *p&lt; 0.05, **p&lt;0.01, two-tailed unpaired t-test, n = 8-10/group"
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "cell cycling status of LT-HSCs after administration of Noggin to HO-1+/+ or HO-1-/- mice."
NCBI gene IDs:
"HO-1: 15368"
Figure legend: "A: Brightfield images of a NTH-KO and NTH/GFP ookinete, showing normal ookinete morphology, but lack of pigment clusters associated with crystalloids (arrows) in the NTH null mutant. Scale bar = 5μm."
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "B: Aligned dot plot of oocyst numbers in Anopheles stephensi infected with parasite lines NTH/GFP, NTH-KO and NTH∆PP. Horizontal lines mark mean values (n=20); ns, not significantly different (Mann-Whitney)."
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "C: Brightfield and fluorescence images of oocysts of parasite lines NTH/GFP and NTH-KO at 15 days post-infection, showing lack of sporulation in the NTH null mutant. Hoechst DNA staining (artificially depicted red) labels parasite nuclei. The large nuclei of the epithelial midgut cells also show. Scale bar = 20μm."
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "D: Aligned dot plot of oocyst diameters in Anopheles stephensi at 15 days post-infection with parasite lines NTH/GFP, NTH-KO and NTH∆PP. Horizontal lines mark mean values (n=20). ** mark statistically significant differences (P&lt;0.0001, Mann-Whitney); ns, not significant."
NCBI gene IDs:
"GFP: 
NTH: 3424314"
Figure legend: "E: Images of ookinetes of parasite lines NTH∆PP showing dispersed NTH∆PP::GFP fluorescence; LAP3/mCherry showing focal LAP3::mCherry fluorescence in crystalloids; Cross (carrying modified alleles encoding NTH∆PP::GFP and LAP3::mCherry), showing dispersed LAP3::mCherry fluorescence. Arrow marks pigment cluster associated with crystalloids. Scale bar = 5μm."
NCBI gene IDs:
"LAP3: 
mCherry: 
NTH: 3424314"