EMBO/SourceData huggingface dataset:
Figure legend: "A-B. Quantitation of ID8 Trp53-/-;Pten-/- 1.15 spheroids  expressing shScramble, shArf5 or shArf6, 6hr time  intervals over 72 hrs. (A) Heatmap (viridis) - area presented as mean of Z-score values, normalised to control (shScramble). (B) Frequency of Spherical  and Hyper-protrusive phenotypes. Heatmap (grayscale) - phenotype proportion (z-score) in control. Heatmap (blue-red) - log2 fold change from control. P-values, bubble size (Cochran-Mantel-Haenszel test with Bonferroni adjustment). Black dot, homogenous effect across independent experiments (Breslow-Day test, Bonferroni adjustment, non-significant). N=3 independent experiments, 4-5 technical replicates/experiment. C. Representative phase contrast images of spheroids  described in (A-B). Outlines pseudocoloured for classification (Spherical, green; Hyper-protrusive, blue). Magnified individual spheroids  from boxed regions at indicated timepoints. Arrowheads, protrusions  into ECM. Scale bars, 400μm or 17μm, as indicated."
NCBI gene IDs:
"Arf5: 11844
Arf6: 11845
Pten: 19211
Trp53: 22059"
Figure legend: "Representative confocal  images and intensity profiles of ID8 Trp53-/- and Trp53-/-;Pten-/- 1.15 cells expressing mNeonGreen  (mNG)-tagged ARF6    (green) and F-actin (magenta) at the invasive front of wounded monolayers Scale bar, 5μm. Tips  for which ARF6-mNG  and F-Actin intensity profiles were measured are annotated, ECM  to body, yellow arrow."
NCBI gene IDs:
"Pten: 19211
Trp53: 22059"
Figure legend: "Representative confocal  images and intensity profiles of ID8 Trp53-/- and Trp53-/-;Pten-/- 1.15 cells expressing mNeonGreen  (mNG)-tagged ARF6  (green) and  F-actin (magenta) in spheroids  (max. projection ~ 10 Z-slices) Scale bar, 5μm. Tips  for which ARF6-mNG  and F-Actin intensity profiles were measured are annotated, ECM  to body, yellow arrow."
NCBI gene IDs:
"Pten: 19211
Trp53: 22059"
Figure legend: "Heatmap, (J) ARF6 interactors  across genotypes. White to blue colour or blue to red, ARF6 interaction  score, Log2Fold Student's t-test Difference in LFQ intensity compared to control ID8 Trp53-/-;Pten-/- 1.15 TurboID  alone. Interactors, sorted, descending order of mean interaction. N=4 independent lysate preparations from each sub-line."
NCBI gene IDs:
"TurboID: 
Pten: 19211
Trp53: 22059"
Figure legend: "C. Western blot, β1-integrin  (ITGB1), pS473-AKT, AKT, ARF6  from deconvolved ITGB1  sgRNA-expressing cells. VCL, loading control for ITGB1, sample integrity control for other blots. Representative blots  of n=3 independent lysate preparations. D. Quantitation of (C). Data, mean ± SD for pS473-AKT:total AKT  band intensity ratio, total AKT  or ARF6  intensity, normalised to control (sgNT ID8 Trp53-/-;Pten-/- clone 1.15) cells. P-values, unpaired, two-tailed t-test."
NCBI gene IDs:
"ITGB1: 16412
Pten: 19211
Trp53: 22059"
Figure legend: "E-F. Frequency of Spherical  and Hyper-protrusive phenotypes in ID8 Trp53-/-;Pten-/- 1.15spheroids upon CRISPR-mediated KO of (E) Itgβ1  or (F) Agap1, 6hr time  intervals over 72 hrs. Heatmap (grayscale) - phenotype proportion (z-score) in control (sgNT). Heatmap (blue-red) - log2 fold change from control. P-values, bubble size (Cochran-Mantel-Haenszel test with Bonferroni adjustment). Black dot, homogenous effect across independent experiments (Breslow-Day test, Bonferroni adjustment, non-significant). N=3 independent experiments, 1-5 technical replicates/experiment."
NCBI gene IDs:
"CRISPR: 
Agap1: 347722
Itgβ1: 16412
Pten: 19211
Trp53: 22059"
Figure legend: "H. Representative confocal  images of Trp53-/- and Trp53-/-;Pten-/- clone 1.15 spheroids  expressing sgNT, sgAgap1  (sg3) or sgItgb1 (sg4), stained  for  collagen IV  (grayscale) and F-Actin (magenta). Boxed areas, basement membrane region in higher magnification. Arrowheads, Collagen IV labelling that is: well-defined, green; fragmented, yellow; absent, navy. Scale bar, 53μm. I. Quantitation of (H). Collagen IV basement membrane staining  as Defined, Fragmented, or Absent in spheroids  set up across n=3 independent experiments, 1 technical replicate/experiment, 5-9 fields imaged per technical replicate, 365 spheroids  scored in total. Data, mean ± SD of % of spheroids  in each phenotype for independent experiments, with circles representing technical replicates. Unpaired t-test, p values annotated."
NCBI gene IDs:
"Agap1: 347722
Itgb1: 16412
Pten: 19211
Trp53: 22059"
Figure legend: "C. Western blots of ID8 Trp53-/-;Pten-/- 1.15 cells expressing either sgNT or sgAgap1 (sg3) and either mNeonGreen  (mNG) or CRISPR-resistant mNG-Agap1_S or -L isoforms. Blotted  for ARF6, pS473-AKT, AKT, mNG, and VCL. VCL  is loading control for AKT, pS473-AKT  and ARF6  and sample integrity control for others. n=3 independent lysate preparations. D. Quantitation of (C). Data, mean ± SD for ARF6  and pS473/AKT  band intensity ratio, normalised to sgNT. P-values, unpaired two-tailed t-test, annotated when significant."
NCBI gene IDs:
"CRISPR: 
mNeonGreen: 
mNG: 
Agap1: 347722
Agap1: 116987
Pten: 19211
Trp53: 22059"
Figure legend: "E-F. Quantitation of ID8 Trp53-/-;Pten-/- 1.15 spheroids  treated with sgNT or AGAP1-targeting sg3 and expressing either mNG  or mNG-fusion with either isoform of AGAP1, 6 hr time  intervals over 72 hrs. (E) Heatmap (viridis) - area presented as mean of Z-score values, normalised to control (sgNT). (F) Frequency of Spherical  and Hyper-protrusive  phenotypes. Heatmap (grayscale) - phenotype proportion (z-score) in control. Heatmap (blue-red) - log2 fold change from control. P-values, bubble size (Cochran-Mantel-Haenszel test with Bonferroni adjustment). Black dot, homogenous effect across independent experiments (Breslow-Day test, Bonferroni adjustment, non-significant). N=3 independent experiments, 5-6 technical replicates/experiment."
NCBI gene IDs:
"mNG: 
AGAP1: 347722
AGAP1: 116987
Pten: 19211
Trp53: 22059"
Figure legend: "ARF6  and AGAP1   mRNA levels in LCM normal ovarian surface epithelium versus HGSOC epithelium  or normal ovarian stroma   versus OC-associated stroma. Specific datasets, sample size (n) and p-values (Mann-Whitney) annotated, whiskers Min-Max, line at median."
NCBI gene IDs:
"AGAP1: 116987
ARF6: 382"
Figure legend: "K-O. Overall survival (% patients, months; TCGA OV  dataset), of patients  grouped by low (M1) versus high (M2) levels, based on a median split, of (K) ARF6  mRNA, (L) AGAP1  mRNA, (M) AGAP1  Exon 14 percentage spliced in ratio (PSI), (N) combination of ARF6  and AGAP1  mRNA, or (O) combination of ARF6  mRNA and AGAP1  Ex14 PSI. Median survival, sample size (n) and p-value, Log-rank test (Mantel-Cox) annotated."
NCBI gene IDs:
"AGAP1: 116987
ARF6: 382"
Figure legend: "A-B. Immunofluorescence  and confocal  imaging of Trp53-/-;Pten-/- 1.15 spheroids stained  for α5-integrin  or β1-integrin (grey or FIRE LUT), Hoechst  (blue) and F-actin (magenta). Magnified images from boxed regions shown. Arrowheads, labelling at protrusion tips. Scale bars, 5 μm. Representative of n=3 spheroids imaged. (B) Intensity profiles for integrins (grey) and  F-actin (magenta) from spheroids  in (A). Tip  measured is annotated, ECM  to body, yellow arrow, tip, white arrowhead."
NCBI gene IDs:
"Pten: 19211
Trp53: 22059"
Figure legend: "C-D. Immunofluorescence  and confocal  imaging of Trp53-/-;Pten-/- 1.15 spheroids stained  for pFAK (Y379) or pSRC Family Kinases (SFK  pY416) (grey or FIRE LUT), Hoechst  (blue) and F-actin (magenta). Magnified images from boxed regions shown. Arrowheads, positive staining. Scale bars, 5 μm. Representative of n=5 spheroids  imaged. (D) Intensity profiles for active FAK  and Src  (grey) and F-actin (magenta) from spheroids  in (C). Tip  measured is annotated, ECM  to body, yellow arrow, tip, white arrowhead."
NCBI gene IDs:
"Pten: 19211
Trp53: 22059"
Figure legend: "E-H. Representative capture ELISA  graphs (E,G) and associated quantitation (F,H) for recycling of internalized cargoes between Trp53-/- versus Trp53-/-;Pten-/- cells or Trp53-/-;Pten-/- cells expressing shScramble versus shArf6 for active β1-integrin. Graphs shown are representative of n=2 (E) or n=3 (G) independent replicates. Data, mean (black square) ± SD for repeated experiments (large circles), 1-3 technical replicates/experiment/timepoint  (small circles), two-tailed t-test, p-values are annotated."
NCBI gene IDs:
"Arf6: 11845
Pten: 19211
Trp53: 22059"
Figure legend: "I-K. Overall survival  (% patients, months; TCGA OV  dataset) of patients  grouped into combined expression based on median mRNA split. (I) Low (red line, M1) or high (blue line, M2) expression for all mRNA, control, remaining patients  (green line), (J), same as (I), but CYTH2  Ex9 PSI, rather than total CYTH2. (K), as for (I), but PTEN  protein levels split by quantiles (red and blue, Q1+Q2,Q3, low PTEN, green Q4, high PTEN). Median survival, sample size (n) and p-value, Log-rank test (Mantel-Cox) annotated."
NCBI gene IDs:
"CYTH2: 9266"
Figure legend: "CY, but not bendamustine treatment, led to suppression of CBP, TIP60, MORF, and MSL1 in H1650 cells. Cells were treated with DMSO, bendamustine (350 μM), CY (15 μM), or SAHA (10 μM) for 6 h, and mRNA was extracted for qPCR analysis. Data represent mean ± SEM from three independent experiments, and statistical significance was determined by unpaired two-tailed t-test. ***P &lt; 0.01."
NCBI gene IDs:
"CBP: 1387
TIP60: 10524
MORF: 23522
MSL1: 339287"
Figure legend: "ShRNA-mediated suppression of CBP, TIP60, MORF, and MSL1 at mRNA level. Data represent mean ± SEM from three independent experiments, and statistical significance was determined by unpaired two-tailed t-test. *P &lt; 0.1, **P &lt; 0.05, ***P &lt; 0.01."
NCBI gene IDs:
"CBP: 1387
TIP60: 10524
MORF: 23522
MSL1: 339287"
Figure legend: "Suppression of CBP, TIP60, MORF, and MSL1 sensitized cells to bendamustine. Y-axis represents relative resistance calculated from results of GFP competition assays, and statistical significance was determined by unpaired two-tailed t-test. Data represent mean ± SEM from two independent experiments. **P &lt; 0.05, ***P &lt; 0.01."
NCBI gene IDs:
"CBP: 1387
TIP60: 10524
MORF: 23522
MSL1: 339287"
Figure legend: "B CY exhibited enhanced activity against BCR-ABL Arf−/− murine ALL cells in vitro."
NCBI gene IDs:
"ABL: 11352///11350
BCR: 110279
Arf: 12578"
Figure legend: "C, D CY showed superior antitumor activity compared with bendamustine, SAHA, and other chemotherapeutic drugs in mice transplanted with BCR-ABL ALL cells. P-values of CY versus other drugs: NT (P = 5.02E-06) (C), SAHA (P = 4.45E-06), Bend (P = 5.18E-06), NT (P = 1.20E-05) (D), VCR (P = 1.13E-05), Dox (P = 8.08E-06), AraC (P = 7.62E-06), and CTX (P = 1.13E-05). Survival statistical analysis was done with the Mantel-Cox (log-rank) test of GraphPad Prism."
NCBI gene IDs:
"ABL: 11352///11350
BCR: 110279"
Figure legend: "E Therapeutic effects of CY (weekly dose) and targeted drugs PP242 (daily dose) and dasatinib (daily dose) in mice transplanted with BCR-ABL ALL cells. P-values of CY versus other drugs: NT (P = 3.94E-05), PP242 (P = 1.20E-05), and dasatinib (P = 0.99). Survival statistical analysis was done with the Mantel-Cox (log-rank) test of GraphPad Prism."
NCBI gene IDs:
"ABL: 11352///11350
BCR: 110279"
Figure legend: "Toxicity of CY190602 in patients harboring deletion of chromosome 17p (including p53 locus) compared to patients without del17p. Viability of samples was normalized to background apoptosis for individual patient samples. For all experiments, cell viability was determined after 72 h of drug treatment. Data represent mean ± SD."
NCBI gene IDs:
"p53: 7157"
Figure legend: "Immunoblot analysis showed HEK293 transfected for 48 hours with expression vectors for TCHP, and FLAG-TCHP at N- and C- terminal. total lysates were immunoprecipitated with anti-FLAG antibodies, and blots were probed sequentially with anti-PCM1, anti-FLAG, and anti-ACTIN antibodies. IgG light chains are indicated with Red Ponceau staining. The input totals were analyzed by parallel immunoblotting as a control for the level of expression."
NCBI gene IDs:
"FLAG: 
TCHP: 84260"
Figure legend: "Immunoblot analysis showed HEK293 transfected for 48 hours with expression vectors for TCHP-FLAG C-terminal or constructs with deletions of the coiled-coil domain 1 (TCHP ∆1) and 2 (TCHP ∆2), as indicated in the scheme. total lysates were immunoprecipitated with anti-FLAG antibodies, and blots were probed sequentially with anti-PCM1, anti-FLAG, and anti-ACTIN antibodies. IgG light chains are indicated with Red Ponceau staining. The input totals were analyzed by parallel immunoblotting as a control for the level of expression."
NCBI gene IDs:
"FLAG: 
TCHP: 84260"
Figure legend: "Co-localization of TCHP-V5 and centriolar satellite proteins. HUVECs were transduced with TCHP-V5 lentivirus and stained for anti-PCM1, anti-CEP290, anti-CEP72 and anti-V5 antibodies. Scale bars, 25 µm and 5 µm in the inset. Right panel: the two-channel intensity correlation of pixels corresponding to regions identified with TCHP-V5 and satellite markers (n=90 cells; Pearson colocalization coefficient)."
NCBI gene IDs:
"V5: 
TCHP: 84260"
Figure legend: "TCHP knock-down or control cells were stained for anti-PCM1 antibody. Panel below: quantification (n=50 cells; unpaired t-test; **p=0.004 vs control). Scale bars, 50 µm and 5 µm in the inset."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Control or TCHP knock-down HUVECs were subjected to cycloheximide (CHX) and MG132 treatments for the indicated number of hours before immunoblotting. PCM1 levels were analysed in the insoluble fraction. Laminin has been used as a loading control. Below panel: Quantification of PCM1 degradation. (one-way ANOVA; *p=0.0180 vs control time 0)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Control or TCHP knock-down HUVECs were subjected to cycloheximide (CHX) and MG132 treatments for the indicated number of hours before immunoblotting. Condition as in (A), Western blot for anti-GABARAP and anti-ACTIN antibodies. Below panel: Quantification of GABARAP degradation. (one-way ANOVA; *p =0.0215 vs control time 0)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Control or TCHP overexpressing HUVECs were subjected to CHX treatment for the indicated number of hours prior to immunoblotting. Western blot was probed for anti-PCM1 and anti-V5 antibodies. Laminin has been used as a loading control."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Control or TCHP overexpressing HUVECs were subjected to CHX treatment for the indicated number of hours prior to immunoblotting. Western blot was probed for anti-V5, anti-GABARAP and anti-ACTIN antibodies. Below panels: Quantification of C (one-way ANOVA; **p&lt;0.0001 vs control time 0; ##p=0.0003 vs control CHX) and D (one-way ANOVA; **p&lt;0.0001 vs control time 0; ##p=0.0056 vs control CHX)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Immunofluorescent staining for LC3 and p62 in TCHP knock-down and control cells. Scale bars, 25μm. Lower panel: quantification. (n=90 cells, unpaired t-test; LC3: **p&lt;0.0001 vs control; p62: **p&lt;0.0001 vs control)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Western blot of p62 and LC3 during under normal culture condition or starved condition (HBSS) or the presence of BafA1 (2 hours) in TCHP knock-down or control cells. Lower panels: p62 quantification. (one-way ANOVA; **p=0.0003 vs control, ##p=0.0093 vs shTCHP); LC3 quantification. (one-way ANOVA; **p=0.0008 vs control, ##p=0.0011 vs shTCHP)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "HUVECs were transduced with the tandem mCherry-EGFP-LC3 and with shRNA TCHP or control vectors. Left panels: Representative picture of mCherrry-EGFP-LC3 reporters. Scale bars, 25μm. Right panel: Quantification of the number of mCherry-only (red bars, autolysosomes) or double-positive (mCherry+/EGFP+; yellow bars, autophagosomes). (n=80 cells, one-way ANOVA; *p=0.0447 vs control; ##p=0.0007 vs shTCHP)."
NCBI gene IDs:
"EGFP: 
mCherry: 
LC3: 84557
TCHP: 84260"
Figure legend: "Ratiometric flow cytometric analysis of mCherrry-EGFP-LC3 reporters as in C. (one-way ANOVA; *p=0.0247 vs control; ##p=0.0088 vs shTCHP)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Quantification of long-lived protein degradation assay in TCHP knock-down and control cells by flow cytometry (one-way ANOVA; **p=0.0018 vs control, ##p&lt;0.0001 vs shTCHP)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "HUVECs were transduced with TCHP-V5 and control vectors. Western blot for anti-V5 and anti-p62 antibody during under normal culture condition or HBSS or BafA1 or Torin-1 treatment. Lower panel: quantification. (one-way ANOVA; **p=0.0044; #p=0.0117 vs TCHP-V5)."
NCBI gene IDs:
"V5: 
TCHP: 84260"
Figure legend: "The postnuclear fraction (PNS) from TCHP knock-down and control HUVECs in the presence or absence of BafA1 was separated into (low-speed pellet) LSP and (high-speed pellet) HSP fractions and then analysed by immunoblots using anti p62, LC3 and GABARAP antibodies. The sub-fractions were treated with proteinase K (Prot. K) with or without Triton X- 100 (TX-100). Quantification of the Western blot: the p62, LC3 and GABARAP levels relative to respective GAPDH were quantified using densitometry analysis and normalized to the value of non-treated samples."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Representative TEM images of autophagosomes in control and TCHP knock-down HUVECs in the complete medium after 3h incubation with BafA1 (Scale bars, 600nm): i, quantification of mean autophagic vesicles (AVs) and autophagosome (APs) area and ii, distribution of the cross-section areas of the analysed vesicles expressed in percentage. (n=7 cells; &gt;100 vesicles per sample; AVs: unpaired t-test; **p&lt;0.0001 vs control; APs: unpaired t-test **p&lt;0.0001 vs control)."
NCBI gene IDs:
"TCHP: 84260"
Figure legend: "Left panels: Representative single-channel and merged images of HUVECs expressing GFP-LC3 and immunostained for STX17. Scale bars, 25 µm and 2 µm in the inset. Right panels: Quantification of LC3/STX17 positive cells in the presence or absence of BafA1 (one-way ANOVA; **p=0.0060 vs control; #p=0.0136 vs control BafA1) and quantification LC3/STX17 positive puncta per cells (n=50 cells; one-way ANOVA; **p&lt;0.0001 vs shTCHP BafA1)."
NCBI gene IDs:
"GFP: 
LC3: 84557
TCHP: 84260"
Figure legend: "Expression of TCHP, p62, IL-1β, IL-8, and IL-6 in ECs from vessels wall from patients with CAD (n=8 patients per group, unpaired t-test; TCHP **p &lt;0.0001; p62 **p = 0.0007; IL-1β **p &lt;0.0001; IL-8 **p &lt;0.0001; and IL-6 **p &lt;0.0001 vs. Healthy subject)."
NCBI gene IDs:
"IL-8: 3576
IL-1β: 3553
IL-6: 3569
p62: 8878
TCHP: 84260"
Figure legend: "Expression of IL-6, IL-8 and IL-1β (n=3 patients per group; one-way ANOVA; IL-6: *p = 0.0135 vs healthy DMSO; #p = 0.0180 vs patients DMSO; IL-8: *p = 0.0139 vs healthy DMSO; ##p = 0.0024 vs patients DMSO; IL-1β: *p = 0.0104 vs healthy DMSO; ##p = 0.0069 vs patients DMSO)."
NCBI gene IDs:
"IL-8: 3576
IL-1β: 3553
IL-6: 3569"
Figure legend: "Left panels: representative images of the heart of wild-type and Tchp knock-out mice stained for CD31 (green) and p62 (magenta). Scale bars, 100µm, and 50µm for the inset. Right panels: Quantification of the percentage of cardiac area (n=4 mice per group; unpaired t-test; **p=0.083 vs wild-type) or vessels positive for p62 (n=4 mice per group; unpaired t-test; **p=0.058 vs wild-type) and vessel density (n=4 mice per group; unpaired t-test; **p=0.0097 vs wild-type)."
NCBI gene IDs:
"Tchp: 77832"
Figure legend: " F qPCR analysis of Efnb2, Cdh5 and Kdr expression (normalized to Actb) in aged human endocrine glands compared to young glands. Data information: (n=4), P-values, and two-tailed unpaired t-tests for all the above panels. ns: not significant; *: P &lt; 0.05; **: P &lt; 0.01; ****: P &lt; 0.0001. Human samples from donors at ages below 20 years and over 70 years were chosen for young and aged group sets, respectively. "
NCBI gene IDs:
"Actb: 60
Cdh5: 1003
Efnb2: 1948
Kdr: 3791"
Figure legend: " E Representative 3D images of pancreas from adult Vegfr2i∆EC mutant and control mice with Insulin, CD31 and Emcn immunostainings. F Combo plot (left) with quantification of CD31+ Emcnhi vessel density in Vegfr2i∆EC and control pancreatic islet. Right combo plot with insulin estimation in pancreas by ELISA assay. Nuclei: DAPI or TO-PRO-3 as indicated. Is: pancreatic islet. f.u: fluorescence unit."
NCBI gene IDs:
"Vegfr2: 16542"
Figure legend: " G Representative 3D images in the upper panel show CXCR4, Ki67 and VE-Cadherin in islets from Vegfr2i∆EC mutant and control mice. 3D images (lower panel) show Vegfr2i∆EC mutant and control islets immunolabeled with Insulin and VE-Cadherin. H Combo plot (top) shows the percentage of CXCR4+ β-cells per islet in Vegfr2i∆EC mutant and control pancreatic islet. Combo plot (middle) shows the quantification of Ki67+ β-cells normalized to the number of β-cells. Combo plot (bottom) shows the percentage of Insulin+ β-cells per islet. Nuclei: DAPI or TO-PRO-3 as indicated. Is: pancreatic islet. f.u: fluorescence unit."
NCBI gene IDs:
"Vegfr2: 16542"
Figure legend: " I Graph shows qPCR analysis of Pdgfa, Pdgfb, Igf1, Igf2, Cxcl12, Hgf and Kitl expression (normalized to Actb) in purified CD31+ Emcnhi ECs compared to CD31+ Emcnlo ECs. "
NCBI gene IDs:
"Actb: 
Cxcl12: 20315
Hgf: 15234
Igf1: 16000
Igf2: 16002
Kitl: 17311
Pdgfa: 18590
Pdgfb: 18591"
Figure legend: " K Bar graphs show qPCR analysis of Dll4, Emcn, Kdr, Flt4, Sele, Mmp9, Plxnd1, Robo4, Unc5b, Cdh5, Cldn5, Gja1, Gja4, Icam1, Icam2, Jam3, Ntn1, Ntn2, Ocln, Pecam1, Tjp1, Tjp2, Tjp3 and Vcam1 expression (normalized to Actb) in purified CD31+ Emcnhi ECs compared to CD31+ Emcnlo ECs from murine pancreas. "
NCBI gene IDs:
"Actb: 
Cdh5: 1003
Cldn5: 12741
Dll4: 54485
Emcn: 59308
Flt4: 14257
Gja1: 14609
Gja4: 14612
Icam1: 15894
Icam2: 15896
Jam3: 83964
Kdr: 3791
Mmp9: 17395
Ntn2: 102467474
Ntn1: 18208
Ocln: 18260
Pecam1: 18613
Plxnd1: 67784
Robo4: 74144
Sele: 20339
Tjp1: 21872
Tjp2: 21873
Tjp3: 27375
Unc5b: 107449
Vcam1: 22329"
Figure legend: " L Graph shows qPCR analysis of Gja1 expression (normalized to Actb) in purified CD31+ Emcnhi ECs from juvenile, adult and aged murine pancreas. "
NCBI gene IDs:
"Actb: 
Gja1: 14609"
Figure legend: " A Representative tile scan 3D images with α-SMA, Isolectin, FABP4 and Emcn in the pancreas from 17-weeks-old Gja1i∆EC mutant and control mice. Insets show higher magnifications of regions. B Box whiskers, and scatter plot (left) shows the quantification of CD31+ Emcnhi vessel density in Gja1i∆EC mutant and control pancreatic islets. (n=7), P-value, and two-tailed unpaired t-test. Combo plot (right) shows the quantification of P-Histone H3+ EC numbers per islet (f.u) counted on thick murine pancreatic sections. (n=5), P-value, and two-tailed unpaired t-test. C 3D images show CD31 and Emcn immunostaining in the Gja1i∆EC mutant and control pancreas. The scale bars are 50 µm. D Combined violin, scatter and sample distribution plot shows the quantification of β-cell mass (%) in pancreatic islets from Gja1i∆EC and control mice. Data represent mean ± s.d. (n=140 islets from 7 pancreas), P-value, and two-tailed unpaired t-test. E Representative 3D images with immunostaining of α-SMA, Insulin and VE-Cadherin in the pancreatic islets from Gja1i∆EC and control mice. The scale bars are 50 µm. F Combo plot (left) shows insulin measurement in lysate of pancreas by ELISA. Combo plot (right) shows the percentage of Insulin+ β-cells per islet in Gja1i∆EC mutant and control mice. (n=5), P-values, and two-tailed unpaired t-tests. Data information: The dashed lines in panel A represent the outlines of pancreas. The dashed lines in panel C, E, G, H and I represent the outlines of pancreatic islets. Two-tailed Student's t-test were performed for the statistical analysis. **: P &lt; 0.01; ****: P &lt; 0.0001. Nuclei: DAPI Is: pancreatic islet. f.u: fluorescence unit. The line on the right side of the combo plots represent the sample distribution. Scale bars are 200 µm for tile scan images of longitudinal thick sections across the entire glands, and 50 µm for the higher magnification insets images."
NCBI gene IDs:
"Gja1: 14609"