EMBO/SourceData huggingface dataset:
Figure legend: "(C) WT, Pml-/-, Mk2-/- and Pml-/-Mk2 -/- BMDMs were treated with zVAD (20 μM), AT-406 (1 μM), Nec-1 (40 μM) and GSK872 (10 μM), as indicated, for 18 h. Cell viability was determined by ATP release. Data show mean ± SD of three biological replicates. *P &lt; 0.05 for multiple t-tests according to the Holm-Sidak method. (D) WT, Pml-/-, Mk2-/- and Pml-/-Mk2 -/- BMDMs were treated with TNF (100 ng/ml), zVAD (20 μM), Nec-1 (40 μM) and GSK872 (10 μM), as indicated, for 16 h. Cell viability was determined by ATP release. Data show mean ± SD of three biological replicates. *P &lt; 0.05 for multiple t-tests according to the Holm-Sidak method. "
NCBI gene IDs:
"Mk2: 17164
Pml: 18854"
Figure legend: "(F) WT, Pml-/-, Mk2-/- and Pml-/-Mk2 -/- mice were injected i.v. with TNF (1.5 mg/kg), and survival was monitored for 24 h. Data represent a combination of two independent experiments (n=10). *P &lt; 0.05 for Log-rank (Mantel-Cox) test."
NCBI gene IDs:
"Mk2: 17164
Pml: 18854"
Figure legend: "(D) WT and Pml-/- BMDMs were treated with TNF (100 ng/ml) + zVAD (20 μM) for the indicated times. p38 MAPK was immunoprecipitated, and its association with MK2 was then analysed."
NCBI gene IDs:
"Pml: 18854"
Figure legend: "(B) Expression level of GFP-p62 in HeLa cells stably expressing GFP-p62 (S-GFP-p62) compared with the level of endogenous p62 in the parent HeLa cells."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(D) Bodies containing either endogenous p62 or transiently or stably transfected GFP-p62 all contain polyubiquitin. HeLa cells were fixed and stained with p62 and polyubiquitin (clone FK1) mAbs directly coupled with AlexaFluor555 (red) and AlexaFluor488 (green), respectively. Cells expressing GFP-p62 were only stained for polyubiquitin (red). Bars, 20 μm."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(B) S-GFP-p62 cells were transiently transfected with the indicated myc-tagged p62 constructs, fixed, and stained for the myc tag. Bars, 20 μm."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(A) Localization of bodies containing endogenous p62 or transiently expressed GFP-p62 relative to EEA1-positive early endosomes. Endogenous p62 were stained green using p62 antibodies directly labeled with AlexaFluor488, and EEA1 was stained red with EEA1 mAbs directly labeled with AlexaFluor555. Alternatively, transiently expressed GFP-p62 was expressed in HeLa cells, and EEA1 was stained red with EEA1 mAb (bottom). The boxed area is shown to the right at a higher magnification."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(B) The levels of p62 and LC3-II change with autophagic activity. HeLa or S-GFP-p62 cells were either left untreated or rapamycin (10 μg/ml) or bafilomycin A1 (Baf. A1; 10 μg/ml) was added for 18 h. Immunoblots were sequentially probed using LC3, p62, and actin antibodies."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(C and D) The amount of p62 located to cytoplasmic bodies increases upon inhibition of autophagy. HeLa cells or S-GFP-p62 HeLa cells were left untreated or bafilomycin A1 was added for 18 h, the cells were fixed, and p62 was either stained red using a p62 mAb (C) or imaged directly (D). Nuclei were visualized using the Draq5 DNA stain. The settings for imaging were identical for the treated cells and the untreated control."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(A) HeLa cells transiently transfected with GFP-LC3 alone or cotransfected with siRNA against p62, HA-p62, or HA-p62 D69A were either left in normal medium or starved for amino acids for 1 h. The cells were fixed, and p62 was stained using a p62 mAb. More than 200 randomly selected cells for each condition were scored for the cytoplasmic pattern of GFP-LC3 as either diffuse or punctuate. The frequency of GFP-LC3-positive cells with punctuate localization are indicated to the right."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(B) Endogenous p62 as well as coexpressed myc-tagged wild-type p62 or a UBA deletion mutant of p62 coimmunoprecipitated with GFP-LC3 from HeLa cell extracts. GFP or GFP-LC3 was immunoprecipitated from total cellular extracts after cotransfecting the indicated constructs. The cell cultures were either left untreated or starved for amino acids for 1 h as indicated. Copurified endogenous or ectopically expressed myc-tagged p62 constructs were detected using the p62 mAb. Bars, 20 μm."
NCBI gene IDs:
"p62: 8878"
Figure legend: "(A) HeLa cells transiently expressing a Flag-tagged NH2-terminal fragment (amino acids 1-171) of huntingtin containing a 68-polyglutamine expansion, Flag-N-HttQ68. After 48 h, the cells were fixed, and Flag-N-HttQ68 was stained green using a Flag mAb. Endogenous p62 was stained red using the p62 pAb."
NCBI gene IDs:
"Htt: 3064"
Figure legend: "(C and D) The p62 shell surrounding GFP-N-HttQ68 was detected independently of the use of antibodies. (C) GFP-N-HttQ68 and DsRed-tagged p62 were cotransfected into HeLa cells, and images of live cells were obtained after 48 h of expression. The left panel shows one confocal plane of a representative GFP-N-HttQ68 and DsRed-p62 double-positive structure, and the right panel shows the Z-stack of planes with side views of the aggregate at the side and at the top. N, nucleus. (A-C) Nuclei were detected using Draq5. (D) GFP-N-HttQ68 and tdTomato-tagged p62 were cotransfected into HeLa cells, and images of live cells were obtained after 24 h of expression."
NCBI gene IDs:
"Htt: 3064"
Figure legend: "(B) The effect of coexpressing either a p62 antisense construct or a deletion construct of p62 lacking the UBA domain on N-HttQ68-induced cell death in HeLa and SHSY-5Y neuroblastoma cells."
NCBI gene IDs:
"Htt: 3064
p62: 8878"
Figure legend: "(E) Control or iRapKO MEFs were treated with or without 2 μM 4-Hydroxytamoxifen (4-OHT) for 4 days to induce Raptor knockout. Treated cells were deprived of serum for 2 hr, followed by insulin (100 nM) stimulation for 10 min, and samples were analysed by Western blot."
NCBI gene IDs:
"Raptor: 74370"
Figure legend: "(F) SIN1 WT was expressed in SIN1 KO MEFs, and cells were selected by FACS. Cell lines were deprived of serum for 2 hr, followed by insulin (100 nM) stimulation for 10 min, and samples were analysed by Western blot."
NCBI gene IDs:
"SIN1: 227743"
Figure legend: "The active fragment of mTOR was incubated with Flag-RagC WT purified from HEK-293E cells. The kinase reaction was performed in the presence or absence of mTOR inhibitor Torin or Rapamycin. Samples were analysed by Western blot for pRagC S21"
NCBI gene IDs:
"Flag: 
RagC: 64121"
Figure legend: " (A) The HA-RagC HeLa Flp-In T-Rex stable cell lines were created (See methods for details) and the expression of RagC WT or mutants was induced by addition of tetracycline overnight. Cells were deprived of serum for 2 hr followed by insulin (100nM) stimulation for 20 min. Lysates were analyzed by Western blot for levels of the specified proteins and the phosphorylation state of S6K1. Graphs indicate the mean ± S.E.M. of quantitative analyses of the western blots after normalization for total protein (* p&lt;0.05, # no significant difference, two tailed Student's t test, n=3). (B) Expression of RagC WT, 3A or 3E was induced by addition of tetracycline overnight. Cells were serum starved for 2 hr followed by insulin (100nM) stimulation for 20 min. Lysates were analyzed by Western blot for the levels of the specified proteins and the phosphorylation state of S6K1. Graphs indicate the mean ± S.E.M. of quantitative analyses of the western blots after normalization for total protein (* p&lt;0.05, # no significant difference, two tailed Student's t test, n=3) "
NCBI gene IDs:
"HA: 
RagC: 64121"
Figure legend: " (C) Expression of RagC WT and 3A was induced by addition of tetracycline overnight. Cells were serum starved for 2 hr followed by insulin (100nM) stimulation for indicated time. Left: Lysates were analyzed by Western blot for the levels of the specified proteins and the phosphorylation state of S6K1. Right: Graph shows mean ± S.E.M. of quantitative analyses of the Western blots after normalization for total protein, n=3 "
NCBI gene IDs:
"RagC: "
Figure legend: " (D) HEK-293E cells stably expressing RagC WT, 3A or 3E were deprived of total amino acids for 1hr followed by addition of amino acids for 10 min. Lysates were analyzed by Western blot for the levels of the specified proteins and the phosphorylation state of S6K1. Graphs indicate the mean ± S.E.M. of quantitative analyses of the western blots after normalization for total protein (* p&lt;0.05, ** p&lt;0.01, two tailed Student's t test, n=3) "
NCBI gene IDs:
"RagC: "
Figure legend: " (A) Expression of RagC WT, 3A, or 3E was induced by overnight addition of tetracycline in HeLa cells followed by cell size analysis using a particle size counter (Horizontal lines, box limits and whiskers represent the median, the 25th percentile ot the 75th percentile and the minimum to the maximum respectively. * p&lt;0.05, # no significant difference, One-way ANOVA with Bonferroni multiple comparisons, n=4) "
NCBI gene IDs:
"RagC: "
Figure legend: " (B) Phospho-RagC positively regulates wing compartment size in Drosophila. Over-expression (OE) of dRagC WT, 3A, or 3E in wing posterior compartment using en-Gal4 driver and the posterior compartment sizes were measured in each genotype ((Horizontal lines, box limits and whiskers represent the median, the 25th percentile ot the 75th percentile and the minimum to the maximum respectively. ** p&lt;0.01, *** p&lt;0.001, **** p&lt;0.0001, One-way ANOVA with Bonferroni multiple comparisons, n ≥ 63 flies per condition) "
NCBI gene IDs:
"en: 
Gal4: 
RagC: 35793"
Figure legend: " (C) Empty vector (EV), RagC WT, 3A or 3E were transiently expressed in stable RagC knockdown HEK-293E cells followed by cell size analysis using a particle size counter (Horizontal lines, box limits and whiskers represent the median, the 25th percentile ot the 75th percentile and the minimum to the maximum respectively. * p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001, # no significant difference, One-way ANOVA with Bonferroni multiple comparisons, n=2 for C2 and n=4 for other conditions) "
NCBI gene IDs:
"RagC: "
Figure legend: " (D) Empty vector (EV), HA-RagC WT, 3A or 3E were expressed in HEK-293E cells followed 48 hr later by incubation with DMEM in the presence or absence of serum for 4 hr. Lysates were analyzed by Western blot for phosphorylation of ULK1 and total ULK1"
NCBI gene IDs:
"HA: 
RagC: "
Figure legend: " (E) Empty vector (EV), HA-RagC WT, 3A or 3E were expressed in HEK-293E cells followed 48 hr later by incubation with DMEM in the presence or absence of serum for 4 hr. Chloroquine (100μM) was added to media 30 min prior to harvest. Lysates were analyzed by Western blot for levels of LC3-II and 14-3-3.Graphs show mean ± S.E.M. of quantitative analyses of LC3-II blotting after normalization for 14-3-3. * p&lt;0.05, two tailed Student's t test, n=4 "
NCBI gene IDs:
"HA: 
RagC: "
Figure legend: " (F) GFP-LC3 was co-expressed with empty vector, RagC WT, 3A or 3E in HEK-293E cells followed 48 hr later by serum deprivation for 4 hr. Chloroquine (100μM) was added to media 30 min prior to harvest. LC3 and RagC were monitored by confocal fluorescence microscopy. Scale bar, 10 µm "
NCBI gene IDs:
"GFP: 
RagC: 
LC3: 440738///81631///84557"
Figure legend: " (A) Empty vector (EV), HA-RagC WT, 3A or 3E were transiently expressed in HEK-293E cells. RagC and lysosome maker, Lamp1, were monitored by confocal fluorescence microscopy. Scale bar, 10 μm "
NCBI gene IDs:
"HA: 
RagC: "
Figure legend: " (B) Empty vector (EV), HA-RagC WT or 3A were transiently expressed with scramble or RagC &amp; RagD shRNA in HEK-293E cells. mTOR and HA-RagC were monitored by confocal fluorescence microscopy. Scale bar, 10 µm "
NCBI gene IDs:
"HA: 
RagC: "
Figure legend: " (C) Empty vector (EV), Flag-RagC WT, 3A or 3E were transiently expressed with HA-RagA in HEK-293E cells. Total cell lysates and amounts of mTOR, Raptor and HA-RagA in the Flag-RagC immunoprecipitates were analyzed by Western blot. Graphs show mean ± S.E.M. of quantitative analyses of Western blots (* p&lt;0.05, two tailed Student's t test, n ≥ 3) "
NCBI gene IDs:
"Flag: 
HA: 
RagA: 
RagC: "
Figure legend: " (D) Expression of HA-RagC WT, 3A, or 3E was induced by overnight addition of tetracycline in HeLa cells. Total cell lysates (TCL) and amount of mTOR in the Raptor immunoprecipitates were analyzed by Western blot. Graphs show mean ± S.E.M. of quantitative analyses of Western blots (*** p&lt;0.001, Student's t test, n=3)"
NCBI gene IDs:
"HA: 
RagC: "
Figure legend: " (A) Relative mRNA levels of FX in CD45+ leukocytes in the peripheral blood of E0771 (abbreviated to E) or LLC tumour-bearing mice. The mean sizes of E0771 and LLC tumours were 9.8 mm and 9.5 mm, respectively. Shown are averages (N = 8 mice/group) with SEM and one-way ANOVA "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (B) Relative mRNA levels of FX in CD45+ leukocytes in various organs such as lung (Lu), Liver (Li), spleen (Sp), bone marrow (BM) and lymph node (Lymph) derived from no tumour-bearing or E0771-bearing mice. &quot;Tumour&quot; stands for mRNA levels of FX in E0771 tumours. Shown are averages (N = 6: organs from no tumour-bearing mice, N = 6: organs from E0771-bearing mice) with SEM and one-way ANOVA"
NCBI gene IDs:
"FX: 14058"
Figure legend: " (E) Quantifications of fibrinogen deposition areas in TCM-stimulating wild-type mouse lungs after injection of Vtn+ HepELs. Vtn+ cells were isolated from E0771-bearing wild-type or FX-/-mouse liver by using anti-Vtn beads (1 x 106 cells /mouse). Shown are averages (N=5/ group, 3 fields/ sample) with SEM. Welch\"s t-test "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (F) Separation of Vtn+/ CD45+ HepELs from tumour-bearing FX+/- and FX-/- mouse livers by a cell sorter (Moflo Astrios). Sorted Vtn+CD45+HepELs cells obtained from a littermate (3 animals each) were pooled, and 8 x 103 Vtn+CD45+HepELs cells were i.v. injected into a tumour-bearing mouse. Right graph shows fibrinogen depositions in the lung. Shown are averages (N = 3/ group, 9 fields/ sample) with SEM. 1-way ANOVA"
NCBI gene IDs:
"FX: 14058"
Figure legend: " (A) Representative immunostaining of fibrinogen depositions in tumour-bearing mouse lungs received an injection of rhodamine-labelled B220+CD11c+NK1.1+HepELs derived from tumour-bearing- FX+/− or FX−/− mice (scale bar, 20 μm) "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (B) Immunohistochemical quantifications of fibrinogen depositions in tumour-bearing mouse lung after an injection of B220+CD11c+NK1.1+cells derived from tumour-bearing- FX+/− or FX−/− mouse liver. Sorted B220+CD11c+NK1.1+cells were prepared from three mice per each genotype, and were i.v. injected. Shown are averages (N = 6/group, 4 sections/mouse) with SEM and Welch&quot;s t-test "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (B) Relative mRNA levels of FX, Vtn and TSP1 in liver B220+CD11c+NK1.1+cells stimulated with LiCM or LuCM. Shown are averages (N = 6/group) with SEM and one-way ANOVA "
NCBI gene IDs:
"FX: 14058
TSP1: 21825
Vtn: 22370"
Figure legend: " (C) Relative mRNA levels of FX in liver B220+CD11c+NK1.1+cells stimulated with various factors. Shown are averages (N = 4) with SEM and one-way ANOVA "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (B) Tumour cell homing in the lungs pre-treated with LiCM-primed B220+CD11c+NK1.1+cells from tumour-bearing WT or FX−/− mouse liver. Representative photos of homing of rhodamine-labelled tumour cells (upper, scale bar, 20 μm). Number of homing tumour cells are shown (lower). Shown are averages (N = 60 sections, 5/group, all field count/section, 12 sections/sample) with SEM and Welch&quot;s t-test "
NCBI gene IDs:
"FX: 14058"
Figure legend: " (C) Tumour cell homing in the lungs pre-treated with activated FX-overexpressed B220+CD11c+NK1.1+cells (FX-OE-HepELs) from tumour-bearing lungs. Number of homing tumour cells after an injection of lung HepELs or FX-OE-HepELs are shown. Shown here are averages (N = 24 sections, 4/group, all field count/section, 6 sections/sample) SEM and one-way ANOVA "
NCBI gene IDs:
"FX: 14058"
Figure legend: "B. Scatter plot illustrates individual  mouse TSPO-PET values derived from a whole brain volume of interest. 8-15 female  mice per group at an average age of 11.1 ± 1.6 months (Grn-/- (n = 8), Trem2-/- (n = 9), Double-/- (n = 10), WT (n= 15)). Data represent mean ± SD. For statistical analysis one-way ANOVA with Tukey post hoc test was used. Statistical significance was set at *, p &lt; 0.05; **, p &lt; 0.01; ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "B. Volcano plot presentation of the differently expressed transcripts in  FCRLS- and CD11b-positive  Grn-/- (n=6) in comparison to WT (n=6) microglia isolated from 6-month-old male  mice. 35 out of 65 analyzed genes are significantly changed more than 20%, with 22 genes upregulated (purple) and 13 genes downregulated (blue). Data information: For statistical analysis the unpaired, two-tailed student`s t-test was performed Statistical significance was set at **, p &lt; 0.01; ***, p &lt; 0.001; ****, p &lt; 0.000; ns, not significant."
NCBI gene IDs:
"Grn: 14824"
Figure legend: "A. Western blot  of  PGRN in whole-cell lysates of WT and  GRN-/- hiMGL. Actin was used as loading control. B. Western blot of TREM2 in whole-cell lysates of WT and GRN-/- hiMGL. Mature (mTREM) and immature  TREM2  (imTREM2) are indicated.  Actin was used as loading control."
NCBI gene IDs:
"GRN: 2896"
Figure legend: "E. ELISA-mediated quantification of sTREM2 in conditioned media of GRN-/- hiMGL upon treatment with Ab1 and Ab2 (20 μg/ml, 40 μg/ml) (n = 3, biological replicates). An isotype antibody (10 μg/ml) was used as a negative control. F.  AlphaLISA-mediated quantification of p-Syk  levels in  GRN-/- hiMGL upon treatment with Ab1 and Ab2 (5 μg/ml, 10 μg/ml) with  liposomes (1 mg/ml) for 5 min. (n = 8, biological replicates). Data represent mean ± SEM. For statistical analysis in E and F one-way ANOVA with Dunnett`s post hoc test, was used to compare untreated, Ab1, and Ab2 (20 µg/ml and 40µg/ml) conditions to the isotype treated condition."
NCBI gene IDs:
"GRN: 2896"
Figure legend: "H. Uptake assay for fluorescent  myelin. GRN-/- hiMGL phagocytose significantly more myelin  as compare to WT  hiMGL. This is reversed upon treatment with TREM2 antagonistic antibodies Ab1 and Ab2 (n=4, biological replicates)."
NCBI gene IDs:
"GRN: 2896"
Figure legend: "E. Transcript levels of DAM gene transcripts significantly altered in GRN-/- hiMGL, treated with  Ab1  or Ab2 in comparison to isotype treatment from the data set in A, normalized to the mean of the WT hiMGL samples (n=4, biological replicates). Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Dunnett`s post hoc test was used to compare  Ab1 and Ab2  (20 µg/ml and 40µg/ml) conditions to the isotype treated condition, Statistical significance was set at *, p &lt; 0.05; **, p &lt; 0.01; ***, p &lt; 0.001; ****, p &lt; 0.0001, *****, p &lt; 0.00001, and ns, not significant."
NCBI gene IDs:
"GRN: 2896"
Figure legend: "A.  Western blot of CatD in total brain lysates from 14-month-old female WT, Grn-/-, Trem2-/-, and Double-/- mice. CatD maturation variants are indicated (sc: single chain; hc: heavy chain; n=3). B., C. Quantification of CatD variants in A normalized to WT (n=3 per genotype). Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Tukey's post hoc test of  Grn-/-, Trem2-/- and Double-/- was used. Statistical significance was set at *, p &lt; 0.05; **, p &lt; 0.01; ***, p &lt; 0.001; ****, p &lt; 0.0001, and ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "D., E. Catalytic activity of CatD in  brain lysates from female 6-month-old (n=4 per genotype) (D) or 14-month-old (n=3 per genotype) (E)  Grn-/-,  Trem2-/-, Double-/- mice normalized to WT. Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Tukey's post hoc test of Grn-/-, Trem2-/- and  Double-/- was used. Statistical significance was set at *, p &lt; 0.05; **, p &lt; 0.01; ***, p &lt; 0.001; ****, p &lt; 0.0001, and ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "F. Immunohistochemical analysis of  lipofuscin (green) in coronal  brain  sections. Representative images of thalamus  are shown. Scalebars = 50 μm. G. Quantification of lipofuscin autofluorescence. Five images per mouse were taken, means were normalized to WT samples (n=3 per genotype, female). Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Tukey's post hoc test of Grn-/-, Trem2-/- and Double-/- was used. Statistical significance was set at *, p &lt; 0.05; **, p &lt; 0.01; ***, p &lt; 0.001; ****, p &lt; 0.0001, and ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "A-D. Volcano plot presentation of lipids and metabolites  upregulated (purple) or downregulated (blue) in total brain homogenates from 6-month-old male Grn-/- (A, n=5), Trem2-/- (B, n=5), Double-/- (C, n=5)  mice  in comparison to WT (n=4), and Double-/- in comparison to Grn-/- mice (D). Counts for each sample were normalized to the mean value of WT followed by a log2 transformation (n=4-5 per genotype). Analyte values were adjusted with an FDR &lt;10% to exclude type I errors in null hypothesis testing. Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Tukey`s post hoc test of Grn-/-, Trem2-/- and Double-/- was used. Statistical significance was set at ***, p &lt; 0.001; ****, p &lt; 0.0001, and ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "E-G. Abundance of BMP species and  glucosylsphingosine (GlcSph) in total brain of 6-month-old  Grn-/-, Trem2-/-, Double-/- and WT mice  (n=4-5 per genotype). H. Glucocerebrosidase (GCase) activity in whole  brain lysates from 6-month-old male Grn-/-, Trem2-/-, Double-/- and WT mice. The linear increase of fluorescence signal was measured and then normalized to WT mice (n=3-6 per genotype). Data information: Data represent mean ± SEM. For statistical analysis one-way ANOVA with Tukey`s post hoc test of Grn-/-, Trem2-/- and Double-/- was used. Statistical significance was set at ***, p &lt; 0.001; ****, p &lt; 0.0001, and ns, not significant."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "F. Morphological analysis of cortical microglia. Representative maximum intensity projections of confocal z-stack images showing IBA1+ microglial cells of female WT, Grn-/-, Trem2-/- and Double-/- mice (scalebar = 50 µm). Arrows point to individual microglia, which are shown as three-dimensional reconstruction, scalebar = 10 µm."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "A. Immunoassay-based quantification of  neurofilament-light chain (NfL) protein levels in CSF  of 6-month-old Grn-/- (n=4), Trem2-/-(n=4), Double-/- (n=4) and WT (n=5) male mice. B. Immunoassay-based quantification of NfL  levels in CSF of 14-month-old Grn-/-, Trem2-/-, Double-/- and WT female mice (n=3 per genotype). Data information: Data in A, B represent mean ± SEM. For statistical analysis in A-B two-way ANOVA with Dunnett`s post hoc test was used the unpaired"
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"
Figure legend: "D. Transcript levels of all significantly changed genes in Double-/- vs. Grn-/- brain  mRNA of 6-month-old and 14-month-old mice  analyzed in C. Grin3b was under detection limit in the 14-month-old cohort. Transcript  expression is normalized to the mean of the WT cohort. Data in D represent mean ± SEM. in D two-tailed student`s t-test was performed."
NCBI gene IDs:
"Grin3b: 170483
Grn: 14824"
Figure legend: "F. Bar graph illustrates individual FDG-PET  values derived from a whole  brain volume of interest. Data represent mean ± SD. 8-15 female  mice  per group at an average age of 10.7 ± 1.5 months (Grn-/- n = 8, Trem2-/- n = 10, Double-/- n = 10, WT n = 15) were used. Data in F two-tailed student`s t-test was performed."
NCBI gene IDs:
"Grn: 14824
Trem2: 83433"