EMBO/SourceData huggingface dataset:
Figure legend: "(D) Left: Representative  immunofluorescence images of DLD1 BRCA2-T207A cells 2h post-irradiation (6Gy), as indicated, hybridized with anti-γH2AX and anti-RAD51  antibodies. (RH)  RNase H. Right: Quantification of the number  of  γH2AX foci (left) or  RAD51 foci (right) per nucleus. When indicated, cells were transfected with a plasmid expressing RNase H1 (RH) 48h prior fixation. The data shown is from at least 400 cells per condition from three independent experiments. For statistical comparison of the differences between the samples we applied a Kruskal-Wallis test followed by Dunn's multiple comparison test and the p-values show significant differences. The red line in the plot indicates the median and each symbol represents a single focus."
NCBI gene IDs:
"RH: 
RNase H: 
RNase H1: 
BRCA2: 675"
Figure legend: "(E) Top: Subcellular fractionation showing amount of  RAD51 in DLD1  stable clones expressing BRCA2-T207A (T207A) in chromatin  fraction. When indicated, cells were irradiated  (2h treatment) and/or transfected with a plasmid expressing  RNase H1 (RH) 48h prior fixation. α-tubulin  and Histone H3 signals are shown as markers of cytoplasmic and chromatin fraction respectively. Bottom: quantification of  RAD51 levels detected by Western blot  with a RAD51 specific antibody in chromatin fraction relative to  RAD51 levels in WCE. Results are presented as the fold change compared to the untreated sample. The data represents the mean ± SD of three independent experiments. Statistical significance of the difference was calculated with unpaired t test and the p-values show the significant difference."
NCBI gene IDs:
"RH: 
RNase H1: "
Figure legend: "(A) STK38 interacts with XPO1. HekRasV12 cells were transiently transfected with myc-STK38(wt) together with either Flag-XPO1(wt) plasmid, Flag-control (ctrl = Sirt3) plasmid or without DNA. 24h later, cells were incubated with Okadaic Acid (OA), (final concentration = 1µM) for 1 hour or with DMSO. Flag fusions were pulled-down and co-immunoprecipited proteins were analyzed by western blotting (WB). Upper panel displays whole cell lysates (WCL) and lower panel represents immunoprecipitated proteins."
NCBI gene IDs:
"Flag: 
myc: 
Sirt3: 23410
STK38: 11329
XPO1: 7514"
Figure legend: "STK38 accumulates in the cytoplasm upon nutrient starvation in a XPO1-dependant manner. (B) HeLa cells were transfected with myc-STK38(wt) plasmid. The next day, cells were incubated with DMEM or EBSS in the presence of XPO1 inhibitors KPT-185 or KPT-330 as indicated (final concentration = 1 µM) or DMSO for 4 hours. Cells were then fixed and stained for myc-tag. Representative images are shown and scale bars are 40 µm."
NCBI gene IDs:
"myc: 
STK38: 11329"
Figure legend: "STK38 accumulates in the cytoplasm upon nutrient starvation in a XPO1-dependant manner. (C) Quantification of myc-STK38(wt) nuclear/cytoplasmic staining (n &gt; 30 cells from 3 independent experiments, mean ± SEM; *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, Mann-Whitney test)."
NCBI gene IDs:
"myc: 
STK38: 11329"
Figure legend: "XPO1 activity is required for nutrient starvation-induced autophagy. (E) HeLa cells stably expressing the GFP-LC3-RFP-LC3ΔG reporter autophagic probe [26] were incubated with DMEM or EBSS in the presence of XPO1 inhibitors KPT-185 or KPT-330 (final concentration = 1 µM) or DMSO for 4 hours. The GFP-LC3 (degraded upon autophagy) and RFP-LC3ΔG (non-degraded upon autophagy) signals were recorded by FACS analysis and then shown as a ratio recapitulating overall LC3 level (n=3 independent experiments, mean ± SEM; *p&lt;0.05, **p&lt;0.01, ns, not significant, Mann-Whitney test). Here again, incubation with XPO1 inhibitors significantly impaired the autophagy process"
NCBI gene IDs:
"GFP: 
RFP: 
LC3: 81631"
Figure legend: "STK38 kinase activity is required and sufficient for its cytoplasmic accumulation upon nutrient starvation. (A) HeLa cells silenced for endogenous STK38 were transfected with myc-STK38(wt) expressing plasmid, myc-STK38(K118R) (STK38 kinase-dead version) plasmid or with HA-STK38(PIF) (STK38 constitutively active version) plasmid. 24 hours later, cells were incubated with DMEM or EBSS for 4 hours, fixed and stained for myc-tag or HA-tag. Representative images are shown and scale bars are 40 µm."
NCBI gene IDs:
"HA: 
myc: 
STK38: 11329"
Figure legend: "STK38 kinase activity is required and sufficient for its cytoplasmic accumulation upon nutrient starvation. (B) Graphical representation of tag-STK38 variants nuclear staining/cytoplasmic staining (n &gt; 30 cells from 3 independent experiments, mean ± SEM; **p&lt;0.01, ***p&lt;0.001, ns, not significant, Mann-Whitney test)."
NCBI gene IDs:
"STK38: 11329"
Figure legend: "STK38 kinase activity is required and sufficient for nutrient-starvation induced autophagy. (C) HeLa cells stably expressing the GFP-LC3-RFP-LC3ΔG autophagic probe [26] were transiently transfected with siRNA targeting the 3'UTR region of endogenous STK38 (or with non-targeting siRNA (siNT)). The next day, cells were transiently transfected with the indicated STK38 mutants expressing plasmids 24 hours after plasmid transfection, cells were incubated with DMEM or EBSS for 4 hours. The GFP-LC3 (degraded upon autophagy) and RFP-LC3ΔG (non-degraded upon autophagy) signals were recorded by FACS analysis and then shown as a ratio recapitulating overall LC3 level (n=4 independent experiments, mean ± SEM; *p&lt;0.05, ***p&lt;0.001, ns, not significant, Mann-Whitney test). depleting STK38 prevents autophagy to take place. This effect was partially reversed by expressing the wt ORF as well as constitutively active STK38 whereas kinase-dead version failed to reproduce endogenous STK38 effect. Expression of constitutively active STK38 is sufficient to induce a substantial change in the autophagic flux upon nutrient rich conditions"
NCBI gene IDs:
"GFP: 
RFP: 
LC3: 81631
STK38: 11329"
Figure legend: "STK38 kinase activity is required and sufficient for nutrient-starvation induced autophagy. (D) HeLa cells were transiently transfected with siRNA targeting the 3'UTR region of endogenous STK38 (or with non-targeting siRNA (siNT)) and then transiently transfected with the indicated STK38 mutants expressing plasmids 48h after. The next day, the cells were incubated with DMEM or EBSS for 4 hours and overall p62 level was quantified for autophagy estimation (n = 3 independent experiments, mean ± SEM; ***p&lt;0.001, ns, not significant, Student's t test)."
NCBI gene IDs:
"STK38: 11329"
Figure legend: "(A-B) STK38 is required for XPO1_S1055 phosphorylation. (A) HeLa cells were transiently transfected with the indicated siRNA and subjected to Flag-XPO1(wt) transient transfection the following day. 48 hours later, cells were incubated with Okadaic Acid (OA, final concentration = 1 µM) for 1 hour or with DMSO. Immunoblotting was performed on whole cell lysates with indicated antibodies. (B) Graphical representation of the phospho S1055-XPO1 signal on total XPO1 (n = 3 independent experiments, mean ± SEM; *p&lt;0.05, ns, not significant, Mann-Whitney test). As expected, XPO1 is phosphorylated on its Ser1055 upon OA treatment but not when STK38 is silenced"
NCBI gene IDs:
"Flag: 
STK38: 11329
XPO1: 7514"
Figure legend: "(C) STK38 kinase activity is required for XPO1_S1055 phosphorylation. HekRasV12 cells silenced (or not) for endogenous STK38 were transfected with Flag-XPO1(wt) expression plasmid in addition to myc-STK38(wt) or myc-STK38(K118R) (STK38 kinase-dead version) plasmids. The next day, cells were treated with 1 µM OA or with vehicle (DMSO) for 1 hour. Flag fusions were immunoprecipited and pulled-down proteins were analyzed by western blotting. Upper panel displays immunoprecipited proteins and lower panel represents whole cell lysates."
NCBI gene IDs:
"Flag: 
myc: 
STK38: 11329
XPO1: 7514"
Figure legend: "(A) Graphical representation of XPO1 protein. C528 to S amino acid mutation confers XPO1 resistance to both KPT-185 and KPT-330 chemical inhibitors [24]. S1055 amino acid correspond to the phosphorylation target of the STK38. Finally, the ΔCter region is also highlighted corresponding to a XPO1 construct lacking the 39 C-terminal residues (B-C) XPO1_S1055 phosphorylation is required and sufficient for STK38 nuclear exit upon nutrient starvation. (B) HeLa cells were transiently transfected with myc-STK38(wt) in addition to indicated Flag-XPO1 mutant plasmids. 24 hours later, cells were incubated with DMEM or EBSS for 4 hours both supplemented with KPT-185 (final concentration = 1 µM) in order to inhibit endogenous XPO1 activity. Cells were then fixed and stained for Flag and myc tags. Only cells positives for both Flag-XPO1 and myc-STK38(wt) were captured. Representative images are shown and scale bars are 40 µm. (C) Graphical representation of myc-STK38(wt) nuclear staining/cytoplasmic staining (n &gt; 30 cells from 3 independent experiments, mean ± SEM; ***p&lt;0.001, ns, not significant, Mann-Whitney test). Expression of wt XPO1 failed to induce cytoplasmic localization of STK38 upon nutrient starvation in presence of KPT-185 where C528S mutant recapitulates results described in Figure 2B-C. Expression of phosphonegative XPO1 (S1055A) failed to induces STK38 cytoplasmic localization upon EBSS treatment while phosphomimetics XPO1 (S1055D and S1055E) were sufficient to promote STK38 cytoplasmic localization without autophagic stimuli. Finally, expression of XPO1 lacking in its 39 C-terminal residues mimicked phosphomimetics variants."
NCBI gene IDs:
"Flag: 
myc: 
STK38: 11329
XPO1: 7514"
Figure legend: "(D) HAP1 cells carrying genomic XPO1 mutations of S1055 were subjected for IMDM (nutrient rich medium) or EBSS (starvation) incubation for 4 hours followed by western-blot analysis for p62 level measurement. This result indicates that p62 degradation in starvation conditions was inhibited in XPO1_S1055A HAP1 cells whereas p62 degradation was potentiated in both phosphomimetics variant (S1055D &amp; S1055E) (n = 4 independent experiments, mean ± SEM; *p&lt;0.05, ns, not significant, Mann-Whitney test"
NCBI gene IDs:
"XPO1: 7514"
Figure legend: "(E) Hek-HT-iRFP-LC3 cells were transiently transfected with siRNA targeting endogenous STK38 (or with non-targeting siRNA (siNT)). Two days after, cells were transiently transfected with indicated Flag-XPO1 mutant plasmids. 24 hours later, cells were incubated with DMEM or EBSS for 4 hours both supplemented with KPT-185 (final concentration = 1 µM) in order to inhibit endogenous XPO1 activity and with chloroquine (final concentration = 10 µM). Cells were then fixed and the number of iRFP-LC3 dots per cell was recorded only in cells positive for Flag-XPO1 mutants As expected, silencing of endogenous STK38 prevented the formation of LC3 dots upon starvation where introduction of phosphonegative XPO1 (S1055A) failed also to increase LC3 dots upon starvation. Interestingly, phosphomimetic XPO1 (S1055D) was sufficient to increase the number of LC3 dots even in complete and also STK38-depleted conditions."
NCBI gene IDs:
"Flag: 
iRFP: 
LC3: 81631
STK38: 11329
XPO1: 7514"
Figure legend: "(A) XPO1 and STK38 are required for Beclin1 cytoplasmic accumulation upon nutrient starvation-induced autophagy. HeLa cells were transiently transfected with the indicated siRNA (control and KPT conditions = siNT). 72 hours later, cells were incubated with XPO1 inhibitors KPT-185 or KPT-330 as indicated (final concentration = 1 µM) or with DMSO for all other conditions for 2 hours prior incubation with DMEM or EBSS supplemented (or not) with inhibitors for 2 hours. Cells were fixed and stained for endogenous Beclin1. Graphical representation of endogenous Beclin1 nuclear staining/cytoplasmic staining (n &gt; 30 cells from 3 independent experiments, mean ± SEM; *p&lt;0.05, ***p&lt;0.001, ns, not significant, Mann-Whitney test). XPO1 activity inhibition by both KPT-185 and KPT-330 as well as STK38 silencing failed to induce Beclin1 cytoplasmic accumulation upon nutrient starvation"
NCBI gene IDs:
"STK38: 11329"
Figure legend: "(B) XPO1_S1055 phosphorylation is required and sufficient for Beclin1 nuclear exit upon nutrient starvation. Genome edited XPO1 mutant HAP1 cells were incubated with IMDM (complete) or EBSS (starvation) for 2 hours. Cells were then fixed and stained for endogenous Beclin1. Graphical representation of endogenous Beclin1 nuclear staining/cytoplasmic staining (n &gt; 30 cells from 3 independent experiments, mean ± SEM; ***p&lt;0.001, ns, not significant, Mann-Whitney test). Here, phosphonegative XPO1 failed to induce Beclin1 cytoplasmic accumulation upon nutrient starvation whereas phosphomimetics variant (S1055D &amp; S1055E) potentiated Beclin1 cytoplasmic accumulation"
NCBI gene IDs:
"XPO1: 7514"
Figure legend: "(C) Genome edited XPO1 mutant HAP1 cells were transiently transfected with siRNA targeting endogenous STK38 (siSTK38#206 or with non-targeting siRNA (siNT)). 72 hours later, cells were incubated with IMDM (complete) or EBSS (starvation) for 2 hours. Cells were then fixed and stained for endogenous Beclin1. Graphical representation of endogenous Beclin1 nuclear staining/cytoplasmic staining (n = 30 cells from 2 independent experiments). Here, phosphomimetic XPO1 (S1055D) potentiated Beclin1 cytoplasmic localization in both complete and starvation condition even in the absence of STK38 as compared to cells carrying wt XPO1"
NCBI gene IDs:
"STK38: 11329
XPO1: 7514"
Figure legend: "(A) XPO1 and STK38 are required for YAP1 nuclear export at high confluence. A549 cells were transiently transfected with the indicated siRNA (control and KPT conditions = siNT) at low or high confluency. 48 hours later, cells were incubated overnight in the presence of XPO1 inhibitors KPT-185 and KPT-330 (final concentration = 1 µM) or with DMSO for all other conditions. The next day, cells were fixed and stained for endogenous YAP1. Quantitative representation of YAP1 nuclear fluorescence intensity (n > 300 cells from 3 independent experiments, mean ± SEM; ***p&lt;0.001, Mann-Whitney test). XPO1 activity inhibition by both KPT-185 and KPT-330 as well as STK38 silencing failed to induce YAP1 nuclear exit at high confluence"
NCBI gene IDs:
"STK38: 11329"
Figure legend: "(B) XPO1_S1055 phosphorylation is required and sufficient for YAP nuclear exit. Genome edited XPO1 HAP1 cells were cultured for two days at low versus high confluency. Cells were then fixed and stained for endogenous YAP1. Quantitative representation of YAP1 nuclear fluorescence intensity (n &gt; 300 cells from 3 independent experiments, mean ± SEM; ***p&lt;0.001, Mann-Whitney test). Here, phosphonegative XPO1 failed to induce YAP nuclear exit at high confluence whereas phosphomimetics variant (S1055D &amp; S1055E) potentiated YAP1 nuclear exit"
NCBI gene IDs:
"XPO1: 7514"
Figure legend: "(C) Genome edited XPO1 mutant HAP1 cells were transiently transfected with siRNA targeting endogenous STK38 (siSTK38#206 or with non-targeting siRNA (siNT)) at low or high confluence. 72 hours later, cells were fixed and stained for endogenous YAP1. Quantitative representation of YAP1 nuclear fluorescence intensity (n = 150 cells from 2 independent experiments). Here, phosphomimetic XPO1 (S1055D) potentiated YAP1 nuclear exit at both low and high confluences even in the absence of STK38 as compared to cells carrying wt XPO1"
NCBI gene IDs:
"STK38: 11329
XPO1: 7514"
Figure legend: "C Top: Schematic illustration of the miR-17-92 cluster with colours marking miRNAs belonging to the same miRNA family. Bottom: TaqMan analysis of the mature miR-17-92 miRNAs in Srsf3 knockout (KO) and control (Ctrl) iPSCs (*P&lt;0.05, ***P&lt;0.001, two-tailed unpaired Student's t-test, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"miR-17: 723905
Srsf3: 20383"
Figure legend: "B TaqMan analysis of the six miR-17-92 miRNAs in HEK293T cells overexpressing the WT-miR-17-92, 17/20a-∆CNNC or total-∆CNNC construct (*P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001, One-way ANOVA, mean as ± SEM, n=4, biological replicates)."
NCBI gene IDs:
"miR-17: 
miR-17: 407975"
Figure legend: "C Western blot analysis of HA-tagged SRSF3-FL or SRSF3-∆RS expression in LIM1215 cells (detection with a HA-antibody)."
NCBI gene IDs:
"HA: 
SRSF3: 6428"
Figure legend: "D TaqMan analysis of the six miR-17-92 miRNAs in LIM1215 cells overexpressing empty pCGN vector, SRSF3-FL or SRSF3-∆RS constructs (*P&lt;0.05, **P&lt;0.01, ***P&lt;0.001, ****P&lt;0.0001, One-way ANOVA, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"miR-17: 407975
SRSF3: 6428"
Figure legend: "B Visualization and comparison of WT miR-17-92 SHAPE reactivities in control and SRSF3-KD cells. First panel: Mean control (blue) and SRSF3-KD (red) SHAPE reactivities, calculated over 11nt sliding windows. Second panel: ∆SHAPE reactivity differences between control and SRSF3-KD. Regions with higher reactivity in control are marked with blue shading and regions with higher reactivity in SRSF3-KD with red shading. Third panel: The SRSF3-KD to control reactivity ratios. The black boxes mark the CNNC sites."
NCBI gene IDs:
"miR-17: 407975
SRSF3: 6428"
Figure legend: "G Visualization and comparison of total-∆CNNC miR-17-92 SHAPE reactivities in control and SRSF3-KD cells. First panel: Mean control (grey) and SRSF3-KD (orange) SHAPE reactivities, calculated over 11nt sliding windows. Second panel: ∆SHAPE reactivity differences between control and SRSF3-KD. Regions with higher reactivity in control are marked with grey shading and regions with higher reactivity in SRSF3-KD with orange shading. Third panel: The SRSF3-KD to control reactivity ratios. The red boxes mark the mutated CNNC sites."
NCBI gene IDs:
"miR-17: 407975
SRSF3: 6428"
Figure legend: "B RT-qPCR quantification of Srsf3 and Cdkn1a (p21) mRNA levels in Srsf3-knockout (KO) and control (Ctrl) iPSCs (*p&lt;0.05, ***&lt;0.001, two-tailed unpaired Student's t-test, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"Cdkn1a: 12575
p21: 12575
Srsf3: 20383"
Figure legend: "C Quantification of Cdkn1a mRNA expression by RT-qPCR during reprogramming in Srsf3-KO and control cells (***p&lt;0.001, ****p&lt;0.0001, Two-way ANOVA, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"Cdkn1a: 12575
Srsf3: 20383"
Figure legend: "D RT-qPCR quantification of CDKN1A (p21) levels in HEK293T cells overexpressing empty vector, WT-miR-17-92, 17/20a-∆CNNC and total-CNNC∆ expression constructs (*p&lt;0.05, **p&lt;0.01, One-way ANOVA, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"CDKN1A: 1026
p21: 1026
miR-17: 407975"
Figure legend: "E RT-qPCR quantification of CDKN1A (p21) levels in LIM1215 cells over expressing empty vector, SRSF3-FL and SRSF3-∆RS constructs (**p&lt;0.01, One-way ANOVA, data as mean ± SEM, n=3, biological replicates). F RT-qPCR quantification of CDKN1A (p21) levels in LIM1215 cells overexpressing scrambled control, miR-17, miR-20a or miR-17+miR-20a mimics (**p&lt;0.01, One-way ANOVA, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"miR-20a: 
CDKN1A: 1026
p21: 1026
miR-17: 407975
SRSF3: 6428"
Figure legend: "A Western blot analysis of SRSF3 and CDKN1A expression in SRSF3-T2A-GFP overexpressing (SRSF3) and control (GFP) LIM1215 cells. GAPDH served as a loading control."
NCBI gene IDs:
"GFP: 
SRSF3: 6428"
Figure legend: "B TaqMan analysis of miR-17-92 miRNAs in SRSF3-T2A-GFP overexpressing (SRSF3-GFP) and GFP-only control (GFP) LIM1215 cells (*p&lt;0.05, ****p&lt;0.001, two-tailed unpaired Student's t-test, data as mean ± SEM, n=4, biological replicates)."
NCBI gene IDs:
"GFP: 
miR-17: 407975
SRSF3: 6428"
Figure legend: "C RT-qPCR quantification of CDKN1A (p21) mRNA levels in SRSF3-T2A-GFP overexpressing (SRSF3-GFP) and control (GFP) LIM1215 cells (**p&lt;0.01, two-tailed unpaired Student's t-test, data as mean ± SEM, n=3, biological replicates)."
NCBI gene IDs:
"GFP: 
CDKN1A: 1026
p21: 1026
SRSF3: 6428"
Figure legend: "D Left: Schematic of the Firefly luciferase reporter constructs Right: Luciferase activity in SRSF3 overexpressing (SRSF3-GFP) and control (GFP) LIM1215 cells transfected with the reporter constructs (**p&lt;0.01, ***p&lt;0.001, two-tailed unpaired Student's t-test, data as mean ± SEM, n=4, biological replicates)."
NCBI gene IDs:
"GFP: 
luciferase: 
SRSF3: 6428"
Figure legend: "F Quantification of fraction of cells in G1, S and G2 phases in SRSF3 overexpressing cells (SRSF3-GFP) relative to control (GFP) LIM1215 cells (*p&lt;0.05, **p&lt;0.01, two-tailed unpaired Student's t-test, data as mean ± SEM, n=4, biological replicates)."
NCBI gene IDs:
"GFP: 
SRSF3: 6428"
Figure legend: "A, B RT-qPCR quantification of SRSF3 (A) and CDKN1A (B) mRNA levels in colorectal tumours and their paired normal samples (*p&lt;0.05, **p&lt;0.01, two-tailed unpaired Student's t-test, data as mean ± SEM, n=25, biological replicates). N=normal, T=tumour."
NCBI gene IDs:
"CDKN1A: 1026
SRSF3: 6428"
Figure legend: "D Association between SRSF3 expression and tumour (T) stage. T1 (n=3), T2 (n=5), T3 (n=11) and T4 (n=3) tumours, SRSF3 fold change as the dependent variable. Linear regression analysis (central band=median, boxes =25th and 75th percentile and whiskers=min and max, n=biological replicates). E Association between SRSF3 expression and tumour differentiation status. Moderately (Mod.) differentiated tumours (n=19) and poorly differentiated tumours (n=5), SRSF3 fold change as the dependent variable. Linear regression analysis (central band=median, boxes =25th and 75th percentile and whiskers=min and max; n=biological replicates)."
NCBI gene IDs:
"SRSF3: 6428"
Figure legend: "G SRSF3 and CDKN1A expression in TCGA COAD data (version 2016_01_28; tumour n=459 and normal n=41 (biological replicates); RSEM, RNA-seq by Expectation Maximation; **p&lt;0.01, two-tailed unpaired Student's t-test, central band=median, dotted lines =25th and 75th percentile)."
NCBI gene IDs:
"CDKN1A: 1026
SRSF3: 6428"
Figure legend: "A. qPCR data comparing the expression of the CamkIIδc transgene in the brain and heart of 3 month old CamkIIδc TG mice; n=4/group; *P &lt;0.05, Unpaired t-test, two-tailed. Note that the transgene is not detected in the brain."
NCBI gene IDs:
"CamkII: 24246
CamkIIδc: 24246"
Figure legend: "C. Ejection fraction, cardiac output and index are significantly decreased in CamkIIδc TG mice (n =8) when compared to control mice (n=5; *P &lt;0.05, , **P&lt;0.01, ***P&lt;0.001, Unpaired t-test, two-tailed). Heart to body ratio, left ventricle to body ratio, and lung to body weight ratio are increased in CamkIIδc TG (n=8) compared to control (n=5; *P &lt;0.05, **P&lt;0.01, Unpaired t-test; two-tailed)."
NCBI gene IDs:
"CamkII: 24246"
Figure legend: "H. qPCR quantification of selected genes reflecting ER-stress or protein methylation-related processes in CamkIIδc TG mice (n=4) and control mice (n=5). *p&lt;0.05, ns = non-significant, Unpaired t-test; two-tailed. Data is normalized to Hprt1 expression."
NCBI gene IDs:
"CamkIIδc: 24246
Hprt1: 15452"
Figure legend: "D. The cumulative score of hippocampus-dependent search strategies during Barnes maze training is impaired in CamkIIδc TG (n = 16) when compared to control mice (n = 13; two-tailed, unpaired t-test, *p&lt;0.05). E. Number of visits to escape hole during probe test to assay memory retrieval was impaired in CamkIIδc TG (n = 16) when compared to control mice (n = 13; two-tailed, unpaired t-test, *p&lt;0.05). Data information: Bars and error bar indicates mean ± SEM. "
NCBI gene IDs:
"CamkIIδc: 24246"
Figure legend: "A. Hypergeometric overlap analysis comparing genes deregulated in CamkIIδc TG mice to genes differentially expressed in the hippocampal CA1 region of Kat2A, Kmt2A and Kmt2b knock out mice. Fisher's hypergeometric test, Benjamini-Hochberg (BH) correction."
NCBI gene IDs:
"CamkIIδc: 24246
Kat2A: 14534
Kmt2A: 214162
Kmt2b: 75410"
Figure legend: "E. H3K4me3 at TSS region of genes from RNA module1. Inset shows statistical comparisons among groups (One-way ANOVA, ***P&lt;0.001, ****P&lt;0.0001). Bars and error bars indicate mean ± SEM. Number of replicates analyzed for H3K4me3 profile at TSS (Wildtype vehicle: 3; CamkIIδc TG-vehicle n = 4; CamkIIδc TG-Vorinostat n = 4)."
NCBI gene IDs:
"CamkIIδc: 24246"