EMBO/SourceData huggingface dataset:
Figure legend: "(B) Gel electrophoresis of RT-qPCR analysis loaded at the saturation phase for miR-204 in SEZ culture medium and SEZ medium from the ChP co-culture."
NCBI gene IDs:
"miR-204: 387200"
Figure legend: "(B, C) Micrographs depicting specific expression of AAV5 encoded GFP in the ChP 7 days after viral delivery in the lateral ventricle. (B' and C') are magnifications of the SEZ and (B'' and C'') of the ChP. (D) Dot plot depicting expression of miR-204 in ChP 7 days after second ventricular injection of AAV5 encoding for miR-204-specific TuD compared to scrambled control. "
NCBI gene IDs:
"miR-204: 387200"
Figure legend: "Micrographs depicting immunoreactivity for MEIS2 (E, F) priming factors in the LRCs (BrdU+ only) 7 days after ChP-specific miR-204 inhibition and scrambled control White arrows point out the primed LRCs, the arrowheads label the MEIS2 Dot plots depicting the proportion of LRCs immunoreactive for the priming proteins MEIS2 after ChP specific miR-204 interference."
NCBI gene IDs:
"miR-204: 387200"
Figure legend: "Micrographs depicting immunoreactivity for MCM6 (H, I) priming factors in the LRCs (BrdU+ only) 7 days after ChP-specific miR-204 inhibition and scrambled control White arrows point out the primed LRCs, the arrowheads label the MCM6+ LRCs. Dot plots depicting the proportion of LRCs immunoreactive for the priming proteins MCM6 (J) after ChP specific miR-204 interference."
NCBI gene IDs:
"miR-204: 387200"
Figure legend: "(K, L) Micrographs showing the cellular composition in the SEZ 7 days after injection of AVV5 encoding for scrambled control (K) and miR-204 specific TuD (L). Note the reduction of primed LRCs (BrdU+ only, white arrows in K and L) upon TuD-204 injection. (M) Dot plot depicting the number of LRCs (BrdU+ only) in the SEZ 7 days after miR-204 inhibition. "
NCBI gene IDs:
"miR-204: 387200"
Figure legend: "(B) USH1C transcripts were consistently detected in all tested organs of WT and USH1C pigs, except in the heart and cerebellum. RT-PCR for GAPDH was used as control (Ctrl.)."
NCBI gene IDs:
"GAPDH: 396823
USH1C: 100514274"
Figure legend: "(D) Representative Western blot analysis proved lack of harmonin protein expression in retina and duodenum of USH1C pigs at the age of 3 weeks (3w) (1 piglet, 1 retina, 1 duodenum) and 1 year (1y) (2 pigs, 1 retina each, 3 TRs). Arrow indicates the expected size of harmonin isoform a (72kDa)."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(E) Representative immunofluorescence staining of harmonin (green) of longitudinal cryosection through WT and USH1C pig retinas (2 pigs, 1y, 1 retina each, 3 TRs, scale bar 10µm) demonstrated the absence of harmonin staining in the outer segment (OS) of photoreceptor cells and in the other layers below the outer limiting membrane (OLM, arrow) of the USH1C pig retina."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(A, B) Upregulation of GFAP in the retina of USH1C pigs at an age of 3 weeks (3w). (A) Representative immunofluorescence staining of GFAP in Müller glia cells which extend throughout almost the entire retina from the OLM (arrow) to the ganglion cell layer (GCL) of the retina. The consistent increase of GFAP expression in the Müller glia of USH1C pigs indicates Müller cell activation and gliosis. IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; scale bar: 20 µm. (B) Left panel: Western blot analysis of GFAP protein expression in 3w old USH1C piglets and age matched controls. Anti-actin Western blot was used as loading control. Right panel: Quantification of Western blot bands in 4 gels by the LI-COR Odyssey system revealed a strong increase GFAP expression in 3w USH1C piglets when compared to age-matched WT controls (2 piglets, 3w, 2 retinas each, 2 TRs, error bars represent standard deviation (SD) of the mean, two-tailed Student's t tests, *p&lt;0.05)."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(C, D) Reduced synaptic width in USH1C pigs at an age of 1 year (1y). (C) Fluorescent microscopic analysis of the cone synapse phenotype. Left panel: Representative images of longitudinal sections through WT and USH1C pig retinae stained for the pre-synaptic marker PSD-95 (green) and by fluorescent peanut agglutinin (PNA, red) for cone synaptic pedicles (white arrows) and counter-stained by DAPI for nuclear DNA (blue). Scale bar 10µm. Middle panel: Higher magnification of a PNA-stained cone synaptic pedicle. Synapse width was determined as the maximum extension of consistent PNA signals. Scale bar 1µm. Right panel: Measuring cone synaptic pedicle width in WT and USH1C pigs by applying a Fiji script to PNA-stained sections indicated reduced synaptic width (2 pigs, 1y, 1 retina each, number of examined synapses indicated, error bars represent SD, two-tailed Student's t tests, ***p&lt;0.001). (D) Determining cone synaptic pedicles width by TEM. Left panel: Representative images of retinal cross sections. Scale bar 500nm. Right panel: Quantification of synaptic width confirmed the significantly reduced width of cone synaptic pedicles in USH1C pigs. (1 pig, 1y, 1 retina each, number of examined synapses indicated,"
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(A-C) Ciliary length measured by fluorescence microscopic analysis at 1 year of age (1y). (A) Representative immunostaining of the ciliary marker Gt335 (green) in longitudinal cryosections of PRC ciliary regions in WT and USH1C retinas. Counterstaining by DAPI, scale bar 10µm. (B) Gt335 staining at higher magnification and principle of quantifying ciliary length by Gt335 staining. Scale bar 500nm. (C) Length of connecting cilia (CC) appear elongated in USH1C when applying a Fiji script to immunostained histological sections (2 pigs, 1y, 1 retina each, number of examined connecting cilia is indicated, error bars represent SD, two-tailed Student's t tests, ****p&lt;0.0001)."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(D, E) Measuring CC length in TEM sections. (D) Representative images of longitudinal cross sections and principle of determining CC length. Scale bar 500nm. (E) Elongation of CC length is confirmed in TEM sections of USH1C PRC. (1 pig, 1y, 1 retina each, number of examined connecting cilia indicated, error bars represent SD, two-tailed Student's t tests, ****p&lt;0.0001)."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "Length of primary cilia in dermal fibroblasts. (F) Staining of ciliary structures in fibroblasts stained with ARL13B (ciliary shaft, green) and PCNT2 (ciliary base, red), DAPI (nucleus, blue). Scale bar upper panel 5 µm, lower panel 2.5 µm. (H) Similarly, ciliary length of fibroblasts from a human USH1CR31X/R80fs patient are elongated, compared to a healthy control (7 TR, number of cells examined indicated, KS-test, ****p&lt;0.0001)."
NCBI gene IDs:
"USH1C: 10083"
Figure legend: "(I-M) Outer segment (OS) PRC architecture by TEM of longitudinal retinal sections. (I) At 3w, WT show normal sub-cellular organization, including parallel stacking of photosensitive discs and calyceal processes (black arrows, representative image from 2 pigs, 2 retinas, scale bar 600nm). (J) In 3w USH1C rods, membrane discs appear also in vertical orientation (white arrows, representative image from 2 pigs, 2 retinas, scale bar 750nm). At 1y, interstitial gaps appeared in disc stacks of the OS in USH1C pigs (K, arrow, scale bar 550nm) and vesicles are found at the OS base (L, asterisk, scale bar 500nm). (M) The disc architecture in 1y animals was substantially distorted (arrows, representative image from 2 pigs, 2 retinas, scale bar 850nm)."
NCBI gene IDs:
"USH1C: 100514274"
Figure legend: "(D) Efficacy of AAV gene therapy was examined in vivo by sub-retinal injection of Anc80 virus capsid expressing harmonin_a1 under a ubiquitous promoter into a USH1C pig (see also Figure EV5). Left panel: Sub-retinal injection reconstituted harmonin expression in the AAV-treated eye but not in the sham-treated eye (3 TR). Protein extract from a WT retina served as positive control, actin staining as loading control. The 72kD band correlates with harmonin_a isoforms while higher MW indicates putative dimer formation. Middle panel: Immunofluorescence reveals absence of harmonin in the retina of the PBS-injected control eye and harmonin abundance (green) in the retina of the AAV-treated eye. (Representative image from a single animal. Blue: DAPI, scale bar 25 µm). Right panel: Photopic gfERG reveal increased a- and b-wave response to single flash and flicker stimulation in the AAV-treated (red), compared to the sham-treated (black) eyes of two USH1C pigs."
NCBI gene IDs:
"harmonin_a: 10083
harmonin_a1: 10083
USH1C: 100514274"
Figure legend: "HDAC6 KO MEF cells are defective in aggregate clearance but not in autophagosome induction or targeting. (A) Filter‐trap analysis of MG132‐induced, SDS‐insoluble ubiquitinated aggregates generated in wild‐type (WT), HDAC6 KO, and KO MEFs reconstituted with different HDAC6 constructs as indicated. The signal intensity from the ubiquitinimmunoblot (bottom panel) was quantified and presented as the average of the means from three independent experiments along with the standard deviation (top). Note the significant accumulation of ubiquitin‐positive aggregates in HDAC6 KO MEFs and KO MEFs stably expressing HDAC6‐CD and ΔBUZ mutants. WT, HDAC6 KO, and HDAC6 KO MEFs stably expressing human HDAC6 (hHDAC6 WT), HDAC6 CD (catalytically inactive mutant), or HDAC6 ΔBUZ (ubiquitin‐binding‐deficient mutant) were analysed for the level of HDAC6 using an anti‐human HDAC6 antibody. Mouse endogenous HDAC6 (mHDAC6) and actin levels were determined by each corresponding antibody."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(B) WT and HDAC6 KO MEFs were treated with MG132 and subjected to Western blot analysis for LC3, actin, and HDAC6."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(D) WT and ATG5 KO MEFs are treated with 2.5 μM MG132 for 1 day and incubated with normal growth media for 18 h. MEFs are immunostained with anti‐LC3 (red) and anti‐ubiquitin antibody (green)."
NCBI gene IDs:
"ATG5: 11793"
Figure legend: "(A) Wild‐type and HDAC6 KO MEFs were transfected with pcDNA, pcDNA‐HDAC6WT, HDAC6CD, or HDAC6ΔBuz, along with mCherry‐GFP‐LC3 as indicated. Yellow signals indicate non‐acidic autophagosomes and red signals indicate acidic autophagolysosomes. Scale bar, 10 μm."
NCBI gene IDs:
"HDAC6: 15185
LC3: 66734"
Figure legend: "(C)In vitro fusion assays. Autophagosomes (APGs) and lysosomes (Lys) purified from wild type and HDAC6−/− MEFs were subjected to heterotypic and homotypic in vitro fusion assays (representative fields are shown in Supplementary Figure S3). Values are means+s.e. of the percentages of fusion from three independent experiments (more than 10 images per each experiment)."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(D) EM images of wild‐type and HDAC6 KO MEFs in normal growth conditions. Yellow arrows, autophagosomes; red arrows, autophagolysosomes; green arrowheads, multilamellar bodies."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(A) Autophagosome-lysosome fusion is analysed in wild‐type and HDAC6 KO MEFs with or without starvation (6 h) using mCherry‐GFP‐LC3 as described in Figure 2A."
NCBI gene IDs:
"HDAC6: 15185
LC3: 66734"
Figure legend: "(B) Wild‐type and HDAC6 KO MEFs were cultured in Hank's solution for 3 h followed by immunoblotting with an antibody for LC3, HDAC6, and GAPDH."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(C) Long‐lived protein degradation in wild‐type and HDAC6 KO MEF cells. The degradation of [14C]‐valine labelled long‐lived protein was measured in the presence or absence of 3‐methyl adenine (3MA, inhibits the formation of autophagic vacuoles). The average of percentage degradation from three independent experiments is presented. The error bar represents the standard deviation."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(A) Wild‐type and HDAC6 KO MEFs were treated with MG132 and immunostained with antibodies to Lamp‐1 (a lysosome marker, red) and ubiquitin (green) as indicated. F‐actin was detected by phalloidin (blue). The arrows indicate ubiquitin‐positive aggregates that are surrounded by F‐actin and Lamp‐1."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(B, C) Wild‐type and HDAC6 KO MEFs were transfected with mCherry‐GFP‐LC3, followed by treatment with LatA (100 nM) or nocodazole (250 nM) for 6 h and analysed as described in Figure 3A."
NCBI gene IDs:
"HDAC6: 15185
LC3: 66734"
Figure legend: "(F) Autophagosomes (APGs) and lysosomes (Lys) isolated from HDAC6 KO MEFs were treated or not with latrunculin (LatA) as indicated and subjected to in vitro fusion assay."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(A) Biochemical characterization of autophagic compartments isolated from HDAC6 KO cells. Different subcellular fractions (75 μg protein) isolated from the wild type (WT) were subjected to SDS-PAGE and immunoblotting for the indicated proteins. Hom, homogenate; APG, autophagosomes; APL, autophagolysosomes; Lys, lysosomes."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(C) WT and ATG5 KO MEFs were treated with MG132 and immunostained with antibodies to ubiquitin (green) as indicated. F‐actin was detected by phalloidin (red). The arrows indicated ubiquitin‐positive protein aggregates."
NCBI gene IDs:
"ATG5: 11793"
Figure legend: "(A) Wild‐type and HDAC6 KO MEFs were treated with MG132 and immunostained with antibodies against cortactin (red), ubiquitin (green), and phalloidin for F‐actin (blue) as indicated. The arrows indicated ubiquitin‐positive aggregates that were colocalized with F‐actin and cortactin."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(B) Wild‐type MEFs were transfected with control or cortactin siRNA, treated with MG132, and stained with antibodies for Lap‐1 (red, to label lysosome), or ubiquitin (green) and phalloidin for actin (blue). Note that F‐actin staining at protein aggregates was lost, but lysosomes remained concentrated in cortactin knockdown cells (arrow)."
NCBI gene IDs:
"cortactin: 13043"
Figure legend: "(C) Wild‐type MEFs were transfected with control or cortactin siRNA, treated MG132 2.5 μM for 18 h, and subjected to filter‐trap assay using a ubiquitin antibody. The knockdown level of endogenous cortactin was confirmed by immunoblotting using an antibody against cortactin and GAPDH in the right panel."
NCBI gene IDs:
"cortactin: 13043"
Figure legend: "(D) U2OS cells were transfected with control siRNA and cortactin siRNA. Autophagosome-lysosome fusion was analysed with or without starvation (6 h) using the mCherry‐GFP‐LC3 reporter as described in Figure 3A."
NCBI gene IDs:
"cortactin: 13043
LC3: 66734"
Figure legend: "(E) The mCherry‐GFP‐LC3 plasmid was cotransfected with wild type, 9KQ (acetylation‐mimic), or 9KR (deacetylation‐mimic) cortactin‐expressing plasmids into wild‐type MEFs. Autophagosome-lysosome fusion was analysed as described in Figure 3A."
NCBI gene IDs:
"cortactin: 13043
LC3: 66734"
Figure legend: "(A) HDAC6 KO mice accumulate ubiquitin‐positive protein aggregates in the brain. The hippocampus and cerebral cortex regions from 6‐month‐old wild‐type and ubiquitin KO littermates were subjected to immunostaining with a ubiquitin antibody and counterstained with hematoxylin. The red arrows indicate ubiquitin‐positive neuritic aggregates and black arrows indicate cytoplasmic aggregates. These ubiquitin‐positive structures were rarely observed in control littermates. Scale bar, 50 μm"
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(B) Apoptotic cell death in the cortex and hippocampus region of HDAC6 KO mice as determined by TUNEL staining. Apoptotic cells were only observed in HDAC6 KO mice. Scale bar, 100 μm."
NCBI gene IDs:
"HDAC6: 15185"
Figure legend: "(C) HDAC6 depletion in the Drosophila eye leads to ubiquitin‐positive pathology. Immunostaining for ubiquitin (green) in frontal eye sections of 1‐day‐old (d1) and 30‐day‐old (d30) fly eyes. The eyes of HDAC6‐depleted flies (GMR:GAL4/UAS‐HDAC6RNAi) developed ubiquitin‐ positive cytoplasmic inclusions that become more prominent at day 30 (d30). Blue, DAPI."
NCBI gene IDs:
"GAL4: 
HDAC6: 32461"
Figure legend: "(D) Depletion of HDAC6 in the Drosophila eye leads to age‐dependent degeneration. Light micrographs (left) and corresponding Richardson‐stained frontal eye sections (right) of 1‐day‐old and 30‐day‐old fly eyes. The eyes of control flies (GMR:GAL4/+) and HDAC6‐depleted flies (GMR:GAL4/UAS‐HDAC6RNAi) show normal highly organized ommatidial array at day 1; 30‐day‐old control animals also show no defects, but 30‐day‐old HDAC6‐depleted flies show degeneration with disorganization of the ommatidial array and loss of normal eye architecture ( × 40 and × 80)."
NCBI gene IDs:
"GAL4: 
HDAC6: 32461"
Figure legend: "(B) Relative mRNA levels of KLF10  in the livers  from NAFL  and NASH mice. n=5. Results represent three independent experiments. Data information: * P &lt;0.05, ** P &lt;0.01-. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "(H) Spearman correlations between KLF10 expression   and NAS in liver  tissues from NAFL  and NASH patients  using GEO datasets (GSE48452). Left panel, n=32 (including 17 NAFL patients  and 15 NASH patients). Right panel, n=21 (including 6 NAFL  and 15 NASH patients). Data information: * P &lt;0.05, ** P &lt;0.01-. Results are shown as mean ± SD. Spearman's correlation H)."
NCBI gene IDs:
"KLF10: 7071"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (B) Protein levels  of KLF10  in the livers  (left) and its expression  was normalized to the β-actin (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (C) H&amp;E staining  of liver  sections."
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (D) CD68 immunofluorescence staining  of liver  sections (left) and its quantitation (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (E) Body weight (BW), liver/body weight  ratio, serum AST   and ALT   levels, and hepatic TG  contents of two groups of mice. Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (F) Sirius red staining  of liver  sections (left) and its quantitation (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (G) Relative mRNA levels of genes involved in the hepatic inflammation  and fibrosis. Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on WD/CCl4  for 12 weeks. n=5 per group. (H) Protein levels of TNF-α  in the livers  (left) and its expression  was normalized to the β-actin  (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (J) Protein levels of KLF10  in the livers   (left) and its expression  was normalized to  β-actin (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (K) H&amp;E staining  of liver  sections."
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (L) CD68 immunofluorescence staining  of liver sections (left) and its quantitation (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through tail vein  injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (M) Body weight (BW), liver/body weight  ratio, serum AST  and ALT  levels, and hepatic TG  contents. Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (N) Sirius red staining  of liver  sections (left) and its quantitation (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein  injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (O) Relative mRNA levels of genes involved in the hepatic inflammation  and fibrosis. Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "8-week-old male C57BL/6J mice  were administered with AAV-GFP  or AAV  -Klf10  through  tail vein injection, and then kept on HFD-feeding for 12 weeks. n=5 per group. (P) Protein levels of TNF-α in the livers  (left) and its expression  was normalized to the β-actin (right). Data information: * P &lt; 0.05, ** P &lt; 0.01, *** P &lt; 0.001. Results are shown as mean ± SD. Student's t test"
NCBI gene IDs:
"GFP: 
Klf10: 21847"
Figure legend: "(A) Heatmap of genes in mouse primary hepatocytes  transfected with lentivirus  vectors expressing KLF10  (KLF10OE) or empty vector (Ctrl), P＜0.001. n=5 per group. The color gradient represents log2(Fold change)."
NCBI gene IDs:
"KLF10: 21847"
Figure legend: "(B) Relative mRNA (n=5) and protein (n=3) levels of zDHHC7  in MPHs  expressing KLF10  or empty vector (Ctrl). The protein level of KLF10  or zDHHC7  was normalized to  β-actin. Results represent three independent experiments. Data information: * P &lt;0.05, ** P &lt;0.01, *** P &lt;0.001. Results are shown as mean ± SD. Student's t test (B"
NCBI gene IDs:
"KLF10: 21847
zDHHC7: 102193"
Figure legend: "(C-D) Spearman correlations between KLF10  and zDHHC7  in liver  tissues from NASH  and NAFL patients  using GEO datasets (GSE48452 and GSE61260). In the dataset GSE61260 (C), n=83 (including 38 healthy controls, 21 NAFL patients  and 24 NASH patients). In the dataset GSE48452 (D), n=73 (including 41 healthy controls, 17 NAFL patients  and 15 NASH patients). Data information: * P &lt;0.05, ** P &lt;0.01, *** P &lt;0.001. Results are shown as mean ± SD. Spearman's correlation (C, D)."
NCBI gene IDs:
"KLF10: 7071
zDHHC7: 55625"