EMBO/SourceData huggingface dataset:
Figure legend: "(e) Representative DIC images from control- and bec-1-RNAi embryos at different stages of development (minutes after first division). The yellow arrowheads mark apoptotic corpses. Scale bar, 10 μm. (f) Quantification of apoptotic corpses in control- and bec-1-RNAi embryos; data show ± s.d.; ***P0.0001."
NCBI gene IDs:
"bec-1: 177345"
Figure legend: "(g) Representative images of CED-1::GFP embryos with control or bec-1 RNAi. The yellow arrowheads mark engulfed corpses surrounded by CED-1::GFP. Scale bar, 5 μm."
NCBI gene IDs:
"bec-1: 177345"
Figure legend: "(E) Schematic illustration of the hCST collateral into the cervical cord and confocal images of hCST collaterals (green), stained for vGlut (red) and vGAT (blue) 21 days following spinal cord injury. Insets represent 3D views generated in Imaris of the deconvolved confocal image. (F) Quantification of bouton density and characterization of bouton between control and FGF22 overexpressing mice (n = 4-5 mice per group). ("
NCBI gene IDs:
"FGF22: 67112"
Figure legend: "(C) Schematic illustration and representative confocal image for vGlut2-Cre neuronal labeling (cyan) and hCST collaterals (white). Insets represent 3D views generated in Imaris of the confocal image. Quantification of hCST contacts onto vGlut neurons (n = 4-6 mice per group)."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "(C) Schematic illustration of the hCST collateral exiting into the cervical cord and confocal images showing exiting hCST collaterals in control (left panel) and FGF22 treated (right panel) mice and quantification of the number of hCST collaterals that enter the cervical grey matter 3 weeks following spinal cord injury. (n = 6 mice per group)."
NCBI gene IDs:
"FGF22: 67112"
Figure legend: "(E) Schematic illustration of the boutons on cervical hCST collateral and confocal images showing putative synaptic boutons (arrows) on newly formed cervical hCST collaterals at 3 weeks following spinal cord injury in a FGF22 (right) and a control (left) mice. Quantification of bouton density in control and FGF22 treated mice. (n = 6 mice per group"
NCBI gene IDs:
"FGF22: 67112"
Figure legend: "(G) Confocal images showing contacts between CST collaterals (red) and long propriospinal neurons (magenta) transduced with FGF22 (cyan). Quantification of the contacts between the hCST collaterals and long propriospinal transduced with FGF22 or control virus (DP refers to double positive cells). Insets on the right are magnification of the boxed area on the left. (n = 6 mice per group)."
NCBI gene IDs:
"FGF22: 67112"
Figure legend: "(C) Timeline of the experiment with FGF22 injection right after the onset of the spinal cord lesion (top). Quantification of the functional recovery in the regular (middle) and irregular (bottom) ladder rung test in controls (grey) and FGF22 (orange) treated mice at baseline, at 3 days post injury (&quot;3dpi&quot;), 14dpi and 21dpi after spinal cord injury. (n = 9-10 mice per group). (D) Timeline of the experiment with FGF22 injection 24hrs after the onset of the spinal cord lesion (top). Quantification of the functional recovery in the regular (middle) and irregular (bottom) ladder rung test in control (grey) and FGF22 (orange) treated mice at baseline, at 3 days post injury (&quot;3dpi&quot; or 2 days &quot;2dpi for the late treatment), 14dpi and 21dpi after spinal cord injury. (n = 9-10 per group). (E) Timeline of the experiment with FGF22 injection 5 days after the onset of the spinal cord lesion (top). Quantification of the functional recovery in the regular (middle) and irregular (bottom) ladder rung test in control (grey) and FGF22 (orange) treated mice at baseline, at 3 days post injury (&quot;3dpi&quot;), 14dpi and 21dpi after spinal cord injury. (n = 9-10 per group).). (F"
NCBI gene IDs:
"FGF22: 67112"
Figure legend: "(C) RPL26  mono- and  di-UFMylation  is lost in Chlamydomonas reinhardtii (Cr) uba5  and ufl1  mutants. Liquid TAP cultures were either left untreated (control) or treated for 24 hours with 200 ng/mL tunicamycin. Protein extracts were analyzed by immunoblotting  with anti-UFM1  antibodies. Total proteins were analyzed by Ponceau S staining. 12 hours and 24 hours treatment replicates are shown in Fig. S3C. Right Panel, Quantification of UFMylated RPL26. RPL26 mono-UFMylated; RPL26-(UFM1)2: RPL26 di-UFMylated."
NCBI gene IDs:
"uba5: 5719339
ufl1: 5717654"
Figure legend: "(D) Chlamydomonas reinhardtii   (Cr) UFMylation  pathway mutants are sensitive to ER  stress triggered by tunicamycin. Liquid TAP cultures of wild type (wt), uba5, ufl1  and ire1  mutants were either left untreated (control) or treated for 3 days with 200 ng/mL of tunicamycin. Left panel, representative images of control and treated liquid cultures taken 3-days after incubation. Middle Panel, optical density (OD) 600  (OD600) quantification of each genetic background under control conditions. Bars represent the mean (± SD) of 5 biological replicates. Two-tailed unpaired t-tests were performed to analyze the differences between wild type and mutants. Right Panel, normalized OD600  quantification of each genetic background under tunicamycin  treatment conditions. Bars represent the mean (± SD) of 5 biological replicates. Two-tailed unpaired t-tests were performed to analyze the differences between wild type and mutants. ns, p-value &gt; 0.05; ***, p-value &lt; 0.001. BR: Biological Replicate."
NCBI gene IDs:
"ire1: 5726847
uba5: 5719339
ufl1: 5717654"
Figure legend: "(G)  AtC53cAIM forms more GFP-ATG8A colocalizing  puncta upon ER  stress. Upper Panel, representative confocal  images of transgenic Arabidopsis  seedlings co-expressing C53-mCherry  (magenta), C53sAIM-mCherry and C53cAIM-mCherry  with GFP-ATG8a  in c53  mutant background under normal condition and after tunicamycin  stress. 6-day old seedlings were incubated in liquid 1/2 MS medium with 1% sucrose  supplemented with DMSO  as control or tunicamycin  (10 μg/ml) for 6 hours before imaging. Scale bars, 30 μm. Inset scale bars, 10 μm. Right Panel, Quantification of the C53-autophagosomes  (C53-APG) per normalized Z-stacks. Bars represent the mean (± SD) of at least twenty roots from 3 biological replicates for each genotype and treatment. Unpaired two-samples Wilcoxon test with continuity correction was performed to analyze the differences between wild type and mutants. ***, p-value &lt; 0.001."
NCBI gene IDs:
"ATG8a: 
GFP: 
mCherry: 
c53: 830574
C53: 830574"
Figure legend: "A Relative  mRNA  expression levels of indicated CLEAR network genes in HCT116 cells treated as indicated ((NEN (N) 1.2 µM; Domperidone (Domp, D), Imipramine (Imi), Desipramine (Desi) and Amitriptyline  (Ami), each 30 µM; Clomipramine  (Clomi) 20 µM), determined by qPCR. Data information: In (A) (first (TFE3) and third (CD68) graph (N=3); second (TFE3) and fourth (CD68) graph (N=5, besides NEN+Clomi  (N=4) and NEN+Desi  (N=4)) data are presented as mean (SD) and were analyzed by a one-way ANOVA with Tukey post-hoc test. In the second and the fourth graph, significance is indicated for the comparison of combinatorial treatments (NEN+TCAs) to controls (Ctrl). * p&lt; 0.05; ** p&lt; 0.01;*** p&lt; 0.001; **** p &lt; 0.0001."
NCBI gene IDs:
"CD68: 968
TFE3: 7030"
Figure legend: "C Relative UPP1 mRNA expression  levels in  HCT116 cells either transfected with control (siCtrl) or combined  TFE3- and MITF- targeting (siTFE3/siMITF) siRNAs and treated as indicated (NEN  (N) 1.2 µM, Domperidone  (D) 30 µM, ISRIB 1 µM), determined by qPCR. Data information: data (N=3) are presented as mean (SD) and were analyzed by two -way ANOVA with Tukey post-hoc test"
NCBI gene IDs:
"MITF: 4286
TFE3: 7030
UPP1: 7378"
Figure legend: "F  Immunoblot  of HCT116 whole cell lysate using antibodies against the indicated proteins upon treatment of the cells as indicated for 16h (NEN (low) 0.6 µM or NEN (High) 1.2 µM, Domperidone  (Domp) 30 µM) Cells were transfected either with control (siCtrl) or UPP1-targeting (siUPP1) siRNAs 48h prior to treatment."
NCBI gene IDs:
"UPP1: 7378"
Figure legend: "H Ratio of  toxicity and viability of HCT116 spheroids (3D) treated as indicated (NEN 1.2 µM, Imipramine (Imi), Desipramine (Desi), Amitriptyline (Ami), each 30 µM). Cells were transfected with control (siCtrl) or  UPP1-targeting (siUPP1) siRNAs 24h prior to spheroid formation. Data information: data (N=3) are presented as mean (SD) and were analyzed by two -way ANOVA with Tukey post-hoc test"
NCBI gene IDs:
"UPP1: 7378"
Figure legend: "A Relative mRNA expression levels of DHODH  in HCT116  cells treated as indicated for 16h (NEN (N) 1.2 µM, Domperidone (Domp, D), Imipramine (Imi), Desipramine (Desi), Amitriptyline  (Ami), each 30 µM, Clomipramine (Clomi) 20 µM). Data information: , data are presented as mean (SD) (N=3) and were analyzed by Kruskal-Wallis with Dunn´s post-hoc test (A, left graph), one-way ANOVA with Tukey post-hoc test (A, right graph) In (A), significance is indicated for the comparison of the different treatments to controls. ** p &lt; 0,01; **** p &lt; 0,0001."
NCBI gene IDs:
"DHODH: 1723"
Figure legend: "D) Percentage schizonts (from total number of schizonts in a well +/- s.d.) located in the different hepatocyte sub-populations by NF54, NF135, NF175 on day 3 p.i.. Each dot represents a biological replicate. For each parasite line, at least 100 schizonts were characterized from 2x 96 wells for the final percentage. The values from three biological replicates were used in a RM ANOVA test followed by a Tukey's multiple comparison test. Percentage of schizonts in Z1/hGK++/hGS- hepatocytes is not different between the three parasite strains. Percentage of schizonts in Z2/hGK+/hGS- for NF54 is significantly different from NF135 (p = 0.0015) and NF175 (p = 0.0015). Percentage of schizonts in Z3/hGK-/hGS+ hepatocytes for NF54 is significantly different from NF135 (p = 0.0002) and NF175 (p = 0.0.0002). Data information: * p &lt;0.05; ** p &lt; 0.005; *** p &lt;0.001; **** p &lt;0.0001"
NCBI gene IDs:
"hGK: 2645
hGS: 2752"
Figure legend: "F) Size of schizonts in Z1/hGK++/hGS-, Z2/hGK+/hGS- and Z3/hGK-/hGS+ hepatocytes on day 3 post invasion for the different Pf strains. For each parasite line, at least 100 schizonts were measured from each biological replicate. The median values +/- s.d. (as the sizes within the population is not normally distributed) from three biological replicates were used in a RM ANOVA test followed by a Tukey's multiple comparison test. Data information: * p &lt;0.05; ** p &lt; 0.005; *** p &lt;0.001; **** p &lt;0.0001"
NCBI gene IDs:
"hGK: 2645
hGS: 2752"
Figure legend: "D) Median sizes (+/- s.d.) of NF135 (green), NF175 (purple) and NF54 (blue) schizonts divided based on the presence (solid line) or absence (dashed line) of hGS staining pattern based on high content fluorescence microscopy from three different liver donors where each donor has at least 100 schizonts were measured for each of three biological replicates. A two-way RM ANOVA was performed on the median followed by a Tukey's multiple comparisons test. The significant differences between hGS+ and hGS- population within a strain is showed with asterisks (* p &lt;0.05; ** p &lt; 0.005; *** p &lt;0.001; **** p &lt;0.0001). See Appendix Fig S1 for the raw measurements."
NCBI gene IDs:
"hGS: 2752"
Figure legend: "B) Size difference between the GS positive (closed circle) and negative (open circle) NF135 schizonts after treatment of AIP. Each dot represents the median of a biological replicate where ≥100 schizonts were measured. Three biological replicates were performed with the median from each plotted +/- s.d.. A two-way ANOVA was performed followed by a Dunnett's multiple comparison test with *** = 0.0002 and **** = 0.0001."
NCBI gene IDs:
"GS: 2752"
Figure legend: "C, D Culture dynamics of non‐infected (C) or phage‐infected (D) cultures. Curves depict culture dynamics of strains lacking BREX (black) and BREX‐containing (red) strains of B. subtilis BEST7003, as well as a BREX‐containing strain where the pglX methylase was deleted (green). Axes and error bars are as in Fig ."
NCBI gene IDs:
"pglX: "
Figure legend: "A Western blot  analysis of VSV-G protein levels in Ifnar1+/+ or Ifnar1-/- MEF cells infected with  VSV (MOI=1.0, 24 hrs) immediately after addition of NaCl (+34 mM). Data information: are representative of at least two biological replicates"
NCBI gene IDs:
"Ifnar1: 15975"
Figure legend: "F RT-qPCR analysis of Ifit1,  Isg54 and Isg15 mRNA levels in HEK293T pretreated with additional NaCl  (+34 mM) for 12 hrs and then treated with IFNα (1,000 IU/ml) as indicated. Data information: Data represent mean and SD of three biological replicates, and p values were calculated using two-tailed unpaired Student's t-test For all statistical testing: NS, not signiﬁcant (p &gt; 0.05), **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"Ifit1: 3434
Isg54: 3433
Isg15: 9636"
Figure legend: "N Survival curves of 8-week-old Rsad2+/+ or Rsad2-/- mice administrated with an NSD or HSD for 7 days and then intraperitoneally infected with VSV (1×108 PFU per gram body mouse, n=10). Data information: Data (N) show the mean and SEM of ten biological replicates, and p value was calculated using logrank (Mantel-Cox) tests. For all statistical testing: NS, not signiﬁcant (p &gt; 0.05), **p &lt; 0.01 and ***p &lt; 0.001."
NCBI gene IDs:
"Rsad2: 58185"
Figure legend: "B Western blot  analysis of FH-Viperin protein in stable FH-Viperin-expressing  HeLa  cells pretreated with additional NaCl  (+34 mM) and then treated with  CHX (50 μM) for 6 and 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"FH: 
Viperin: 91543"
Figure legend: "J Western blot analysis of Viperin in 2fTGH cells transfected with control shRNAs (shCtrl) or shRNAs against USP33 (shUSP33) and then treated with IFNα (1,000 IU/ml) for different times. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"USP33: 23032"
Figure legend: "M Western blot  analysis of Viperin protein in Usp33-/- MEF  cells transfected with FH-USP33 (wild type, WT; C194S and H673Q, DM) and then stimulated with mIFNβ (500 IU/ml) for 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"FH: 
Usp33: 170822
USP33: 170822"
Figure legend: "O Western blot analysis of  Flag-Viperin in stable Flag-Viperin-expressing HEK293T  cells transfected with shCtrl or shUSP33 and then treated with CHX (50 μM) as indicated. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Flag: 
Viperin: 91543
USP33: 23032"
Figure legend: "D  Immunoprecipitation analysis of Viperin ubiquitination in HEK293T cells co-transfected with  Flag-Viperin, HA-Ub  and shUSP33, then treated with additional NaCl (+34 mM) for 12 hrs. Data information: Data are representative of at least two biological replicates"
NCBI gene IDs:
"Flag: 
HA: 
Viperin: 91543
USP33: 23032"
Figure legend: "E  Western blot  analysis of  Viperin levels in  2fTGH cells transfected with shCtrl or shUSP33 and then infected with VSV (MOI=1.0, 12 hrs) immediately after addition of  NaCl  (+34 mM). Data information: Data are representative of at least two biological replicates"
NCBI gene IDs:
"USP33: 23032"
Figure legend: "G RT-qPCR analysis of VSV RNA levels in 2fTGH cells transfected with shCtrl or shUSP33 and then infected with VSV (MOI=1.0, 24 hrs) immediately after addition of NaCl (+34 mM). Data information: Data (G) represent mean and SD of three biological replicates, and p values were calculated using two-tailed unpaired Student's t-test For all statistical testing: NS, not signiﬁcant (p &gt; 0.05). ***p &lt; 0.001."
NCBI gene IDs:
"USP33: 23032"
Figure legend: "I Survival curves of 8-week-old Usp33-/- mice fed an NSD  (0.5% NaCl) or HSD (4% NaCl) for 7 days and then infected with VSV (1x108 PFU per gram body mouse, n=10). Data information: ; Data (I) show mean and SEM of ten biological replicates, and p value was calculated using logrank (Mantel-Cox) tests. For all statistical testing: NS, not signiﬁcant (p &gt; 0.05). ***p &lt; 0.001."
NCBI gene IDs:
"Usp33: 170822"
Figure legend: "G Immunoprecipitation analysis of  ubiquitination of Flag-USP33  in p97-WT or p97-KO 2fTGH cells co-transfected with  Flag-USP33 and HA-Ub and then treated with NaCl (+34 mM) for 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Flag: 
HA: 
USP33: 23032
p97: 7415"
Figure legend: "L Immunoprecipitation  analysis of Viperin ubiquitination in  HT1080 cells transfected with shCtrl or shp97 (1# or 2#) and then treated with IFNα (1,000 IU/ml, 12 hrs) immediately after addition of NaCl (+34 mM). Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"p97: 7415"
Figure legend: "N Western blot analysis of  Myc-Viperin in HEK293T cells co-transfected with Myc-Viperin  and shCtrl or shp97, and then treated with additional NaCl (+34 mM) for 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Myc: 
Viperin: 91543
p97: 7415"
Figure legend: "E, F Mass spectrometry analysis of acetylation of Flag-p97  (E) in RAW264.7 transfected with Flag-p97 and then treated with additional NaCl  (+34 mM) or control ddH2O (Ctrl) for 12 hrs. The intensity of p97-K663 site was shown in (F)."
NCBI gene IDs:
"Flag: 
p97: 269523"
Figure legend: "H  Immunoprecipitation analysis of the interaction between  USP33  and Myc-p97 in  HEK293T  cells transfected with Myc-p97 (WT or K663R). Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Myc: 
p97: 269523"
Figure legend: "L Western blot analysis of FH-USP33 levels in p97-KO  HEK293T cells co-transfected with Myc-p97 (WT or K663R) and Flag-USP33, and then treated with additional NaCl  (+34 mM) for 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Flag: 
Myc: 
USP33: 23032
p97: 7415
p97: 269523"
Figure legend: "M Immunoprecipitation  analysis of  Viperin ubiquitination in  p97-KO 2fTGH cells co-transfected with Myc-p97 (WT or K663R), Flag-Viperin and HA-Ub as indicated, and then treated with additional NaCl (+34 mM) for 12 hrs. Data information: Data are representative of at least two biological replicates."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
Viperin: 91543
p97: 7415
p97: 269523"
Figure legend: "Ctrl or Trim21- deficient THP-1s were stimulated for 4 h with 1000 U/ml IFNα and TRIM21 expression measured by immunoblot."
NCBI gene IDs:
"Trim21: 6737"
Figure legend: "WT or TRIM21- deficient THP-1s were stimulated with AdV (50 000 pp/cell) and 20 μg/ml h9C12 or 20 mg/ml IVIg or with 200 ng/well HT-DNA or 10 μM Nigericin for 16h, and IL-1β measured in the supernatant by ELISA."
NCBI gene IDs:
"TRIM21: 6737"
Figure legend: "WT or TRIM21- deficient THP-1s were stimulated with AdV (50 000 pp/cell) and 20 μg/ml h9C12 or 20 mg/ml IVIg or with 200 ng/well HT-DNA or 10 μM Nigericin for 16h, and TNF measured in the supernatant by ELISA."
NCBI gene IDs:
"TRIM21: 6737"
Figure legend: "HMDM were stimulated as in (A), or with 10 ng/ml LPS for 3 h and NLRP3 mRNA expression measured by qPCR. Data shows average ± s.d. of 2 independent donors"
NCBI gene IDs:
"NLRP3: 114548"
Figure legend: "TLR9 mRNA levels from PBMCs, CD19+ve B cells, CD14+ve monocytes or HMDM derived from CD14+ve monocytes were assessed by qPCR and copy number was determined relative to actin copy number (n = 5 mean ± s.e.m)."
NCBI gene IDs:
"actin: 
TLR9: 54106"
Figure legend: "WT, NLRP3, ASC or Caspase-1 - deficient THP-1s were stimulated as in (A) and IL-1β in the supernatant measured by ELISA. Data is representative of two independent experiments."
NCBI gene IDs:
"Caspase-1: 834
NLRP3: 114548
ASC: 29108"
Figure legend: "AIM2 mRNA levels from PBMCs, CD19+ve B cells, CD14+ve Monocytes or HMDM derived from CD14+ve monocytes. were assessed by qPCR and copy number was determined relative to Actin copy number (n = 5 mean ± s.e.m)."
NCBI gene IDs:
"Actin: 
AIM2: 9447"
Figure legend: "THP-1s expressing either a control guide RNA or targeting cGAS and STING were generated and stimulated with 1000 U/ml IFNα for 4 h and protein levels assessed by western blot."
NCBI gene IDs:
"cGAS: 115004
STING: 340061"
Figure legend: "THP-1s deficient in cGAS and STING were stimulated with AdV (50 000 pp/cell) and 20 μg/ml h9C12, 200 ng/ well HT-DNA, 10 μM Nigericin or 10 ng/ml LPS for 16 h. Data shows combined data (mean ± s.e.m) of 3 experiments with absolute protein values (upper panel) or as % cytokine output of KO cells relative to Ctrl treated cells (lower panel), *P-value ≤ 0.05, ***P-value ≤ 0.001 unpaired, two-tailed t-test)."
NCBI gene IDs:
"cGAS: 115004
STING: 340061"
Figure legend: "(A) Representative whole mount images of WT and Cep63T/T brains at P60. Scale bar = 0.2 cm. (B) Telencephalon area of WT and Cep63T/T brains. WT littermates N = 4, Cep63T/T N = 7; two-tailed Mann-Whitney t-test."
NCBI gene IDs:
"Cep63: 28135"
Figure legend: "(C) Representative whole mount images of WT and Sas4cKO brains at P14. Scale bar: 0.2 cm. (D) Telencephalon area of WT and Sas4cKO brains. WT littermates N = 8, Sas4cKO N = 7; two-tailed Mann-Whitney t-test. Black circles represent Nestin-Cre+ animals."
NCBI gene IDs:
"Sas4: 219103
Cre: 2777477
Nestin: 18008"
Figure legend: "(E) Representative images of E14.5 WT, Cep63T/T and Sas4cKO cortices stained with antibodies against α-Tubulin (green), γ-Tubulin (red) and DAPI (blue). Magenta arrows indicate dead cells; white and yellow arrows indicate cells with bipolar and monopolar spindles, respectively. Insets showing zoomed in view of 2 representative cells. Dashed lines showing the orientation of the cleavage plane relative to the ventricular surface. Scale bar = 25 μm. (F) Graph showing the percentage of bipolar, monopolar and multipolar cells at the ventricular surface of E14.5 cortices. WT n = 201 cells, N = 3 embryos; Cep63T/T n = 312 cells, N = 3 embryos; Sas4cKO n = 296 cells, N = 3 embryos; #, chi-square test with *, post-hoc analysis, comparisons are made to WT."
NCBI gene IDs:
"Sas4: 219103
Cep63: 28135"
Figure legend: "(H) Ratio of mitotic (PH3+) to cycling (Ki67+) cells in E14.5 cortices. WT N = 5, Cep63T/T N = 3, Sas4cKO N = 3; two-tailed Mann-Whitney t-test."
NCBI gene IDs:
"Sas4: 219103
Cep63: 28135"
Figure legend: "(J) Representative images from live-cell movies of Nuclear-mCherry+ WT, Cep63T/T and Sas4cKO NPCs. The timing of mitosis began at nuclear envelope breakdown (NEBD) and finished at nuclear envelope reformation. White arrows track the mitotic events of the mother cell from NEBD to the generation of daughter cells following cytokinesis. (K) Plot showing the duration of mitosis in WT, Cep63T/T and Sas4cKO NPCs. Triangles represent individual cells; triangles of the same color represent cells derived from the same embryo. Circles represent the average mitotic duration of cells from each embryo. WT n = 202 cells, N = 4 embryos; Cep63T/T n = 141 cells, N = 3 embryos; Sas4cKO n = 138 cells, N = 3 embryos; two-tailed Welch's t-test."
NCBI gene IDs:
"Sas4: 219103
Cep63: 28135"
Figure legend: "(A-C) Graph showing the mitotic duration of NPCs and the fate of their progeny in primary cultures generated from E14.5 embryos. Each bar represents one cell; its height represents the amount of time the mother cell spent in mitosis; bar color represents the fate of the progeny. Dashed line is set at 30 mins. WT n = 330 cells, N = 4 embryos; Cep63T/T n = 260 cells, N = 3 embryos; Sas4cKO n = 223 cells, N = 3 embryos. (D-F) Percentage of proliferating, arrested and apoptotic progeny within each group of the indicated mitotic duration, calculated from data shown in A-C; groups with n &lt; 20 cells were excluded from this quantification; #, chi-square test with *, post-hoc analysis, within each genotype, comparisons are made to the 0-30min group."
NCBI gene IDs:
"Sas4: 219103
Cep63: 28135"