EMBO/SourceData huggingface dataset:
Figure legend: "(B) Western blot analysis of the recruitment of FLIP, Caspase 8, Caspase 10 and FADD to the TRAIL-R2 DISC in HCT116 Parental, caspase 8 null (CASP8), caspase 10 null (CASP10) or CASP 8/10 cells treated with 4x AMG655 conjugated beads for 90 minutes."
NCBI gene IDs:
"CASP10: 843
caspase 10: 843
CASP 8: 841
CASP8: 841
caspase 8: 841"
Figure legend: "(D) AlphaScreen® assessment of the impact on the FLIP-FADD interaction of unstapled and stapled peptides corresponding to the FADD α1 helix."
NCBI gene IDs:
"FADD: 8772"
Figure legend: "(E) NanoBiT® assay to quantify FLIP/FADD interactions in a U2OS cell line stably expressing the NanoBiT® constructs, FLIP (LgBiT) and FADD (smBiT) following 48 hour silencing of FLIP, FADD, or caspase 8. Western blot analysis was used to confirm successful knockdown of target proteins by siRNA."
NCBI gene IDs:
"caspase 8: 841
FLIP: 8837
FADD: 8772"
Figure legend: "(F) NanoBiT® assay of HCT116 and U20S wild type (WT) and procaspase 8 null (Null) cells transiently co-transfected with FLIP (LgBiT) and FADD (SmBiT) NanoBiT® constructs for 24 hours. Construct expression was analysed by Western blotting."
NCBI gene IDs:
"procaspase 8: 841"
Figure legend: "(A) Western blot analysis of FLIP, Caspase 8 and FADD recruitment to the TRAIL-R2 DISC in U20S parental cells (EV) and 3 independent caspase 8 null clones (#1, #2, #3) after incubation with AMG655 conjugated beads for 30, 60 or 180 minutes."
NCBI gene IDs:
"caspase 8: 841"
Figure legend: "(E) Western blot analysis of FADD recruitment to the TRAIL-R2 DISC in HCT116 cells transiently transfected with an empty vector (EV), Flag-tagged FADD (WT), or Flag tagged FADD with point mutations at either Phe25 (F25A) or His9 (H9G). Western blotting analysis of the soluble unbound fraction was used to monitor transfection efficiency."
NCBI gene IDs:
"Flag: 
FADD: 8772"
Figure legend: "(F) NanoBiT® assay of U20S cells transiently co-transfected with caspase 8 (LgBiT) and either WT FADD smBiT or FADD (smBiT) constructs with a point mutation at either phenylalanine 25 (F25A) or histidine 9 (H9G) for 48 hours. Western blot analysis was used to assess the expression level of each construct."
NCBI gene IDs:
"FADD: 8772"
Figure legend: "(A) Western blot analysis of FLIP, caspase 8 and FADD recruitment to the TRAIL-R2 DISC in parental (WT) and CFLAR deficient (KO) HAP-1 cells treated with AMG655 conjugated beads for either 45 minutes (45'), 90 minutes (90'), 180 minutes (180'), 3 hours (3h), 6 hours (6h) or 9 hours (9h)."
NCBI gene IDs:
"CFLAR: 8837"
Figure legend: "(C) Caspase-8 (i) and -3/7 (ii) activity assays and high content microscopy assessment of cell death (iii) in Parental (PAR) and FLIP knockout (CFLAR CRISPR) cells treated with escalating doses of isoleucine zipper TRAIL (IZ-TRAIL) for 6 hours."
NCBI gene IDs:
"CRISPR: 
CFLAR: 12633
FLIP: 8837
TRAIL: 8794///8793///8797///8795///8743"
Figure legend: "(D) Caspase-8 (i) and -3/7 (ii) activity assays in Parental (PAR) and FLIP knockout (CFLAR CRISPR) cells treated with escalating doses of isoleucine zipper TRAIL (IZ-TRAIL) for 24 hours. (iii) Flow cytometry assessment of cell death induction in Parental (PAR) and FLIP knockout (CFLAR CRISPR) cells treated with IZ-TRAIL for 24h."
NCBI gene IDs:
"CRISPR: 
CFLAR: 12633
FLIP: 8837
TRAIL: 8794///8793///8797///8795///8743"
Figure legend: "(E) Western Blot analysis of Parental (WT) and FLIP knockout (KO) HAP-1 cells treated with 10ng/mL IZ-TRAIL for 0, 3, 6 or 24 hours."
NCBI gene IDs:
"FLIP: 8837
TRAIL: 8794///8793///8797///8795///8743"
Figure legend: "(F) Cell viability assay of Parental (PAR) and FLIP knockout (CFLAR CRISPR cells) treated with an escalating dose (0-1000ng/mL) of IZ-TRAIL for 72 hours."
NCBI gene IDs:
"CRISPR: 
CFLAR: 12633
FLIP: 8837
TRAIL: 8794///8793///8797///8795///8743"
Figure legend: "(B) NanoBiT® assay performed on TRAIL-R2 DISC IPs (90') carried out in U20S Caspase-8 null cells co-transfected with wild-type or F114A FLIP-LgBiT fusion proteins and wild-type or F122A caspase-8-SmBiT fusion proteins."
NCBI gene IDs:
"caspase-8: 841
FLIP: 8837"
Figure legend: "(E) Western blot analysis of size exclusion chromatography experiments in which HCT116 cells transfected with Flag-tagged wild-type (WT) FLIP(S) were treated with 100ng/mL IZ-TRAIL for 3h prior to cell lysis and separation of low and high MW complexes using a size exclusion column. The fractions eluted at 2MDa and &gt;2MDa are presented."
NCBI gene IDs:
"Flag: 
FLIP: 8837
TRAIL: 8794///8793///8797///8795///8743"
Figure legend: "D. Co-immunoprecipitation of FLAG-dmDis3l2 (bait) and GFP-Tailor full-length and truncations expressed in Drosophila S2cells. GFP-Nibbler (Nbr) served as negative control. Domain architecture of GFP-Tailor truncations are indicated."
NCBI gene IDs:
"Tailor: 40847"
Figure legend: "B. In vitro exoribonuclease assay using 5′ radiolabeled RNA substrate and immunopurified wild-type (WT) or catalytic mutant (CM) FLAG-dmDis3l2 (mutation indicated in Figure 1C), incubated for the indicated time and separated on a 15 % polyacrylamide gel followed by phosphorimaging. Quantification of degraded substrate in percent is indicated.C. Change in abundance of 256 different substrate RNAs as determined by high-throughput sequencing of substrates in experiment shown in (B)."
NCBI gene IDs:
"dmDis3l2: 38034"
Figure legend: "A. RNA decay assay using synthetic, 5′ radiolabeled pre-miR-1003, immunopurified dmDis3l2 and wild-type (WT) or catalytic mutant (CM) Tailor. Reactions were incubated for the indicated time and separated by 15% denaturing polyacrylamide gel electrophoresis and subjected to phosphorimaging.B. Quantification of at least three independent replicates of experiment shown in A. Data represent mean SD. Half-life was determined by fitting data to single exponential (dmDis3l2 and dmDis3l2/TailorCM) or sigmoidal (dmDis3l2/TailorWT) reaction kinetics."
NCBI gene IDs:
"miR-1003: 12798529
Tailor: 40847"
Figure legend: "C. RNA decay assay using synthetic, 5′ radiolabeled pre-miR-1003 containing the indicated number or uridine residues at the 3′ end and immunopurified dmDis3l2.D. Quantification of three independent replicates of experiment shown in C. Data represent mean SD. Data were fit to single exponential reaction kinetics.E. Reaction kinetics (kobs) determined in D plotted against number of 3′ terminal uridine residues. Error of fit is shown as SEM."
NCBI gene IDs:
"miR-1003: 12798529"
Figure legend: "A and E. Western blot analysis of genetically modified Drosophila S2 cells. Unmodified S2 cells (wild-type, wt), or S2 cells depleted of Tailor (tailorko) by CRISPR/Cas9 or expressing FLAG-tagged Tailor (TailorOE) were analyzed. Antibodies are indicated. Note, that panels were assembled from two technical replicates of identical lysates (see source data)."
NCBI gene IDs:
"tailor: 40847"
Figure legend: "A and E. Western blot analysis of genetically modified Drosophila S2 cells. Unmodified S2 cells (wild-type, wt), or S2 cells depleted of dmDis3l2 (dmDis3l2ko) by CRISPR/Cas9 or expressing FLAG-tagged catalytic mutant dmDis3l2 (dmDis3l2CM OE) were analyzed. Antibodies are indicated. Note, that panels were assembled from two technical replicates of identical lysates (see source data)."
NCBI gene IDs:
"dmDis3l2: 38034"
Figure legend: "C. Northern hybridization experiment using total RNA from adult whole male flies using probes against 3′ trailer or mature tRNAAla(TGC). Wild-type flies (w1118), or flies bearing a homozygous frame-shift mutation in the first coding exon (dmDis3l2-/-) or a homozygous amino acid-exchange mutation in the catalytic site (dmDis3l2CM) in the endogenous dmdis3l2 locus in the same genetic background were used. Probes against 2S rRNA served as loading control.D. Quantification of four independent biological replicates of experiment shown in C. P-Value determined by Student's t-test."
NCBI gene IDs:
"2S: 
dmDis3l2: 38034
dmdis3l2: 38034"
Figure legend: "E. Exoribonuclease assay using immunopurified dmDis3l2 and in vitro transcribed, 5′ radiolabeled mature tRNA, containing a CCA modification or 3′ trailer-containing tRNA. Reactions were incubated for the indicated time and subjected to polyacrylamide gel electrophoresis and phosphorimaging."
NCBI gene IDs:
"dmDis3l2: 38034"
Figure legend: "Cyclin D3 was depleted using siRNA in Calu3 cells. Cells were infected 48h later with Delta SARS-CoV-2 at MOI 0.01. Cells were washed 4h post-infection and new media were added. Supernatants and cells were collected 48h later for western blot and TCID50. Uninfected cell lysates were used for western blot. Data information: Graphs represent average of n=4 (E) biological replicates. Statistical analysis was performed using one-way ANOVA with Dunnett's multiple comparisons test. NT, non-targeting control. ns, non-significant; ∗p &lt; 0.1; ∗∗p &lt; 0.01; ∗∗∗p &lt; 0.001. Bars indicate mean with SD."
NCBI gene IDs:
"Cyclin D3: 896"
Figure legend: "A549 AT2  cells were depleted for D and A2 cyclins  (A) A representative western blot from lysates of uninfected knock-down cells."
NCBI gene IDs:
"A2 cyclins: 890"
Figure legend: "(E) A549 AT2  cells were transduced with VSV-G  pseudotyped Fucci containing lentiviral  particles and  VSV-G pseudotyped lentiviral  particles containing WT cyclin D3 or T283A mutant cyclin D3 (mutant not degraded by proteasome). Cells were infected 24h later and collected 48h post-infection for flow cytometry analysis of early S and western blot. n=2 biological replicates; Ordinary two-way ANOVA with Sidak's multiple comparisons test: ns, non-significant; ∗∗p &lt; 0.01. Bars indicate mean with SD."
NCBI gene IDs:
"cyclin D3: 896"
Figure legend: "(A) 293T  cells were contransfected with HA  - cyclin D3 and Strep-tag-SARS-CoV-2 E or nsp9, and control plasmid (EV). Immunoprecipitation  was performed using anti-HA  antibody. The immunoprecipitates  were blotted  with anti-Strep, anti-HA, and cyclin D3 antibodies."
NCBI gene IDs:
"HA: 
cyclin D3: 896
nsp9: 43740578"
Figure legend: "(B) 293T  cells were contransfected with HA  - cyclin D3 and Strep-tag-SARS-CoV-2 E, M, both E and M or nsp9. Immunoprecipitation  was performed using anti-HA  antibody. The immunoprecipitates  were blotted  with anti-Strep, anti-HA  antibodies. WCL, whole cell lysate."
NCBI gene IDs:
"HA: 
cyclin D3: 896"
Figure legend: "(C-G) 293T GFP11  cells were transfected with Spike, and/or with Envelope, Membrane, and cyclin D3. 24h post-transfection cells were seeded at a 1:1 ratio with Vero-GFP10  cells and percentage of GFP+ve area (syncytia) were determined 18h later. S, Spike; E, Envelope; M, Membrane; D3, cyclin D3. (C,D) Representative images of GFP+ syncytia. Scale bars: 40μm. (E-G) Quantification of cell-to-cell fusion showing percentage of the GFP+ve area to the acquired total cell area. (E) n=4 biological replicates. (F,G) n = 4 biological replicates; technical triplicates shown; one-way ANOVA with Dunnett's multiple comparisons test: ns, non-significant; ∗∗∗∗p &lt; 0.0001; ∗∗∗p &lt; 0.001; ∗∗p &lt; 0.01 . Bars indicate mean with SD."
NCBI gene IDs:
"GFP10: 
GFP11: 
cyclin D3: 896
Envelope: 43740570
Membrane: 43740571
Spike: 43740568"
Figure legend: " A) L7Cre rd1 retina expressing Lamplight-T2A-mCherry AAV. Anti-mCherry staining (white) was widespread (Left, scale bar = 500µm). Fluorescent cell bodies found in different focal planes consistent with expression in ganglion (Top Right, scale bar = 50µm) and bipolar cells (Bottom Right, scale bar = 50µm). "
NCBI gene IDs:
"mCherry: 
Cre: 2777477"
Figure legend: "(A) Immunoblotting for Dnmt1, Dmnt3a and Dmnt3b in the spinal cords of AR-97Q, AR-24Q, and wild-type mice. (B) Quantitative densitometry analysis indicated that Dnmt1 spinal cord level was up-regulated in AR-97Q mice (n = 3). Dnmt3a and Dnmt3b spinal cord level was not significantly different among wild-type, AR-24Q, and AR-97Q mice (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(C) Quantification of the Dnmts mRNA levels of the spinal cord in wild-type, AR-24Q, and AR-97Q mice using RT-qPCR (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(D and E) Representative immunohistochemical images depicting the expression of Dnmt1, Dmnt3a and Dmnt3b in the spinal cords of male AR-97Q, AR-24Q, and wild-type mice and quantification of the immunoreactivity for Dnmts (n = 25 neurons from three mice of each group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(F) Double-immunofluorescence staining for Dnmt1 and 1C2 in the spinal motor neurons of an AR-97Q mouse."
NCBI gene IDs:
"AR: 367"
Figure legend: "(G) Immunoblotting for Dnmt1 of the skeletal muscle of wild-type and AR-97Q mice. (H) Quantification of the signal intensity of Dnmt1 bands (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(A) Immunoblotting of NSC34 cells expressing AR-24Q or AR-97Q (NSC24Q and NSC97Q, respectively) that were treated with DHT for Dnmt1, Dnmt3a and Dnmt3b, and quantification of the signal intensities of bands corresponding to Dnmt1, Dnmt3a and Dnmt3b (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(B) Relative mRNA levels of Dnmt1 in NSC24Q and NSC97Q cells measured with RT-qPCR (n = 3)."
NCBI gene IDs:
"Dnmt1: 13433"
Figure legend: "(D) RT-qPCR analysis of Dnmt1 in DHT-treated and -untreated NSC97Q cells (n = 3)."
NCBI gene IDs:
"Dnmt1: 13433"
Figure legend: "(E, F) Quantitative assessment of cell viability changes due to the siRNA-mediated knock down of Dnmt1, Dnmt3a or Dnmt3b in NSC97Q cells with DHT (E) or without DHT (F) using the WST-8 assay (n = 3)."
NCBI gene IDs:
"Dnmt1: 13433
Dnmt3a: 13435
Dnmt3b: 13436"
Figure legend: "(A-D) Grip power (A), body weight (B), rotarod performance (C) (Two-way ANOVA with Tukey's test) and survival rate (D) (Kaplan-Meier analysis and log-rank test) of AR-97Q mice treated with or without RG108 (DMSO, n =20; 0.5 mg/dL, n = 20; 2.0 mg/dL, n = 20). All parameters were significantly improved in AR-97Q mice treated with 2.0 mg/dL RG108 compared with those treated with DMSO: *P &lt; 0.05 (grip); *P &lt; 0.05 (body weight); *P &lt; 0.05 (rotarod); and *P &lt; 0.05 (survival)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(E) Representative photograph of a 13-week-old AR-97Q mouse treated with DMSO (left) and an age-matched AR-97Q mouse treated with 2.0 mg/dL RG108 (right). AR-97Q mouse medicated with RG108 maintained muscle volume and body size compared with DMSO-treated AR-97Q mouse of same age. The white compact masses (arrow) of mice head are the cement covering the surgical site."
NCBI gene IDs:
"AR: 367"
Figure legend: "(F,G) Immunoblots (F) and relative intensities (G) of the Dnmt1-immunoreactive bands of spinal cords from AR-97Q mice treated with or without RG108 (n = 3 per group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(H,I) Dnmt1 immunohistochemistry in spinal cord sections from AR-97Q mice treated with or without RG108 (n = 3 per group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(A) Immunohistochemistry of the spinal cords of AR-97Q mice treated with DMSO or RG108 for polyglutamine using a 1C2 antibody. (B) Quantification of 1C2-positive motor neurons in the spinal cords (n = 5 per group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(C) Immunoblotting of the spinal cords of AR-97Q mice treated with or without RG108 for AR. (D) Densitometric analyses to quantify AR accumulation in the spinal motor neurons of AR-97Q mice treated with DMSO or RG108 (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(E) Relative mRNA expression levels of human AR in AR-97Q mice spinal cord with or without RG108 treatment (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "Immunohistochemistry for ChAT in the spinal cord anterior horns of AR-97Q mice (n = 5 per group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "quantitative analysis of the neuron sizes in the spinal cord anterior horns of AR-97Q mice (n = 5 per group)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(H,I) Western blot analysis (H) and relative signal intensities (I) of the ChAT-immunoreactive bands of spinal cords from AR-97Q mice treated with or without RG108 (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(A) Immunoblotting of SH-SY5Y cells stably expressing AR-24Q (SH24Q) or AR-97Q (SH97Q) for Dnmt1, Dnmt3a and Dnmt3b. (B) Densitometry quantification showing that Dnmt1 level was up-regulated in SH97Q cells compared with SH24Q cells (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(F) The relative mRNA levels of CDC25B, GFRA3, NPY, HES5, SCTR, LEF1-AS1 and CABS1, normalized to beta-2 microglobulin, in DHT-treated SH24Q and SH97Q cells were measured using RT-qPCR (n = 3)."
NCBI gene IDs:
"beta-2 microglobulin: 
CABS1: 85438
CDC25B: 994
GFRA3: 2676
HES5: 388585
LEF1-AS1: 641518
NPY: 4852
SCTR: 6344"
Figure legend: "(G) RT-qPCR analysis indicated the decrease of Hes5 mRNA level in DHT-treated SH97Q cells compared with DHT-untreated SH97Q cells (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(I) Hes5 immunoreactivity of spinal motor neurons in wild-type, AR-24Q, and AR-97Q mice (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(J) Hes5 mRNA levels in the spinal cords of wild-type, AR-24Q, and AR-97Q mice (n = 3)."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "DNA methylation levels of the HES5 promoter region of SH97Q cells treated with DHT and RG108."
NCBI gene IDs:
"HES5: 388585"
Figure legend: "HES5 mRNA levels of SH97Q cells treated with DHT and RG108."
NCBI gene IDs:
"HES5: 388585"
Figure legend: "DNA methylation levels of the Hes5 promoter region in DHT-treated NSC97Q cells treated with or without RG108."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "mRNA levels of Hes5 (n = 3) in DHT-treated NSC97Q cells treated with or without RG108."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(E) Hes5 mRNA levels in the spinal cords of AR-97Q mice treated with or without RG108 (n = 3)."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "(F, G) Hes5 immunoreactivity of spinal motor neurons in AR-97Q mice treated with or without RG108 (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(A) Viability of DHT-treated NSC97Q cells treated with Hes5 and control siRNA measured by the WST-8 assay (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(C) Quantitative cell viability analysis revealed that siRNA-mediated Hes5 knock down negated the therapeutic effect of RG108 in NSC97Q cells that were treated with DHT (n = 3), as determined by the WST-8 assay."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "Cell viability measured with the WST-8 assay of DHT-treated NSC97Q cells transfected with a mock plasmid or the Hes5 vector (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "Cell viability measured with immunoblotting of DHT-treated NSC97Q cells transfected with a mock plasmid or the Hes5 vector (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(F) Quantification of LDH release from DHT-treated NSC97Q cells transfected with a mock plasmid or the Hes5 vector (n = 4)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(G) Immunoblots of DHT-administrated NSC97Q cells transfected with a mock plasmid or the Hes5 vector for AR. (H) AR aggregation as quantified by densitometry (n = 3)."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "(A) Immunoblots of NSC34 cells treated with Hes5 siRNA. (B) Quantification of protein levels of pSmad2, Smad2, phospho-IkBα and heat shock factor-1 (Hsf1) (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(C) siRNA-mediated knockdown of Hes5 down-regulated Hes5 protein level (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(E) Protein levels of Smad2, pSmad2, and Hes5 in NSC97Q cells transfected with Hes5 vector (n = 3)."
NCBI gene IDs:
"Hes5: 15208"
Figure legend: "(F) Immunoblots of Smad2, pSmad2, and Hes5 in primary cortical neurons treated with lentiviral vector containing AR24Q and AR97Q (n = 3)."
NCBI gene IDs:
"AR: 367"
Figure legend: "(G) Relative mRNA levels of Hes5 in primary cortical neurons expressing AR24Q and AR97Q (n = 3)."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "(H) Relative mRNA levels of Hes5 in primary motor neurons expressing AR24Q and AR97Q (n = 6)."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "(I) Western blots of Smad2, pSmad2, and Hes5 in primary cortical neurons expressing AR97Q treated with mock plasmid or Hes5-containing lentiviral vector."
NCBI gene IDs:
"AR: 367
Hes5: 15208"
Figure legend: "RAW264.7 macrophages were infected with Mtb WT or Mtb ΔRD1 for 24 h, Ca WT or Ca YL, and Lm WT or Lm Δhly for 60 min. (A) Levels of Rab8A and Rab8A pT72 phosphorylation were analysed by Western blot and quantified by densitometry. Data represent the mean ± SEM of three independent biological experiments. One-way ANOVA followed by Dunnett's test compared to WT strains. ns= non-significant; *p ≤0.05; **p ≤0.01."
NCBI gene IDs:
"RD1: 
hly: 47223624"
Figure legend: "(B) Recruitment of endogenous LRRK2 and EGFP-Rab8A to M. tuberculosis (Mtb) was visualised by confocal microscopy. Scale bar = 5 μm."
NCBI gene IDs:
"EGFP: "
Figure legend: "(B) WT or LRRK2 KO macrophages were treated with 1 mM LLOMe for 30 min and Rab8A and Rab8A pT72 levels were analysed by Western blot."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "LRRK2 WT or LRRK2 KO macrophages were pre-treated or not with 1 μM GSK2578215A and treated with 1 mM LLOMe for 30 min. The number of LRRK2 per cell were monitored by immunofluorescence and high-content imaging. Scale bar = 10 μm. Data represent the mean ± SEM of three to four independent biological experiments. One-way ANOVA followed by Dunnett's test against the untreated WT control. ns=non-significant; **p≤0.01."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "LRRK2 WT or LRRK2 KO macrophages were pre-treated or not with 1 μM GSK2578215A and treated with 1 mM LLOMe for 30 min. The number of Rab8A positive vesicles per cell were monitored by immunofluorescence and high-content imaging. Scale bar = 10 μm. Data represent the mean ± SEM of three to four independent biological experiments. One-way ANOVA followed by Dunnett's test against the untreated WT control. ns=non-significant; **p≤0.01."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "(A) RAW264.7 macrophages were electroporated with EGFP-Rab8A and treated with 1 mM LLOMe for 30 min. Intracellular distribution of Galectin-3, CHMP4B or LC3B was visualised by immunofluorescence. Scale bar = 5 μm."
NCBI gene IDs:
"EGFP: "
Figure legend: "RAW264.7 WT, LRRK2 KO or Rab8A macrophages pre-treated with 1 μM GSK2578215A (GSK inh) were treated with 1 mM LLOMe for 30 min. CHMP4B vesicle numbers were analysed by immunofluorescence and high content imaging. Scale bar = 20 μm. Right panels show the quantification of number of CHMP4B positive vesicles per cell. Mean ± SEM of three independent biological experiments. ns=non-significant, *p≤0.05, **p≤0.01 by One-way ANOVA followed by Sidak's multiple comparisons test."
NCBI gene IDs:
"LRRK2: 66725
Rab8A: 17274"
Figure legend: "RAW264.7 WT, LRRK2 KO or Rab8A macrophages pre-treated with 1 μM GSK2578215A (GSK inh) were treated with 1 mM LLOMe for 30 min. Galectin-3 vesicle numbers were analysed by immunofluorescence and high content imaging. Scale bar = 20 μm. Right panels show the quantification of number of Galectin-3 positive vesicles per cell. Mean ± SEM of three independent biological experiments. ns=non-significant, *p≤0.05, **p≤0.01 by One-way ANOVA followed by Sidak's multiple comparisons test."
NCBI gene IDs:
"LRRK2: 66725
Rab8A: 17274"
Figure legend: "Live cell imaging of LysoTracker positive spots in WT, LRRK2 KO macrophages treated with 1 mM LLOMe, followed by lysosomal recovery after LLOMe wash-out. One representative experiment out of three shown. Differences between slopes in the LLOMe treatment window were estimated using linear regression."
NCBI gene IDs:
"LRRK2: 66725"
Figure legend: "Live cell imaging of LysoTracker positive spots in WT, Rab8A KO macrophages treated with 1 mM LLOMe, followed by lysosomal recovery after LLOMe wash-out. One representative experiment out of three shown. Differences between slopes in the LLOMe treatment window were estimated using linear regression."
NCBI gene IDs:
"Rab8A: 17274"