EMBO/SourceData huggingface dataset:
Figure legend: "B. Effect of USP44 on USP7 levels. HEK293T cells were transfected with MYC-FOXP3. Cells also received combinations of FLAG-USP7 and varying doses of pLVX-USP44 encoding plasmids. FOXP3 was immunoprecipitated using anti-MYC, and immunoblots were analyzed using anti-FLAG, anti-USP44 or anti-MYC antibodies."
NCBI gene IDs:
"FLAG: 
MYC: 
FOXP3: 50943
USP44: 84101
USP7: 7874"
Figure legend: "C. Effect of USP44 and USP7 coexpression on FOXP3 ubiquitination. HEK293T cells were transfected with plasmids encoding MYC-FOXP3, either FLAG-USP44 or FLAG-USP7 or both, His-ubiquitin. Cells were treated with 20 μM MG132 for 4 hrs before being harvested, lysed, and incubated on Ni-NTA beads to pull down ubiquitinated FOXP3, which was visualized by immunoblotting using antibodies specific for MYC."
NCBI gene IDs:
"FLAG: 
His: 
MYC: 
FOXP3: 50943
USP44: 84101
USP7: 7874"
Figure legend: "D. (left panel) iTreg were polarized from human naïve CD4+ T cells over 7 days (n=3 donors /experiment) receiving control (shCK), or shRNA knockdown constructs targeting USP44 (shUSP44-1, -2), or USP7 (shUSP7-1, -2), or both shRNA constructs. Freshly isolated nTregs were treated with either control or shRNA constructs. After transduction with lentivirus for 48 hours, cells were harvested and run SDS-PAGE gels. Protein levels were visualized by immunoblotting using antibodies specific for FOXP3, USP44, USP7 and GAPDH. Shown are representative findings from 3 independent experiments (biological replicates)."
NCBI gene IDs:
"USP44: 84101
USP7: 7874"
Figure legend: "A. Jurkat T cells were transfected with the indicated combinations and relative doses of expression constructs encoding MYC-FOXP3, FLAG-USP44 along with an IL-2 promoter-driven firefly luciferase reporter. After 8 hours stimulation with PMA/Inomycin, cells were lysed and luciferase activity, which was observed and normalized to renilla luciferase activity."
NCBI gene IDs:
"FLAG: 
luciferase: 
MYC: 
FOXP3: 50943
IL-2: 3558
USP44: 84101"
Figure legend: "B. Tregs (CD4+/CD25High) were recovered from the lymph nodes and spleens of wild type C57BL/6 (WT) and USP44-/- mice by FACS purification. Cells were activated with anti-CD3/CD28 agonist antibodies overnight, and RNA was isolated, and cDNA was generated from these samples for the measurement of several signature Treg-associated transcripts by qRT-PCR. The relative expression level of these by WT and KO-derived Tregs is shown."
NCBI gene IDs:
"USP44: 327799"
Figure legend: "C, D. Analysis of the in vitro suppressive function of Tregs isolated from WT mice or age- and sex-matched USP44-/- mice (n=3/group/experiment). Tregs were mixed with CFSE-labeled responder naïve T cells from CD45.1+ C57BL/6 mice (n=3/experiment) for three days. CFSE dilution in CD45.1+ T cells was observed by flow cytometry and the percent suppression of responder cell proliferation by the co-cultured Tregs was determined."
NCBI gene IDs:
"USP44: 327799"
Figure legend: "A. Colitis was induced by intravenous coinjection of CD4+CD25-CD62Lhigh T cells and congenically distinct (CD45.2) CD4+CD25high Treg cells into Rag2-/- mice (n=8/group; 1x106 and 2x105 cells per Rag2-/- recipient, respectively). Changes in body weight over time were monitored and are expressed as a percentage of the original weight."
NCBI gene IDs:
"Rag2: 19374"
Figure legend: "B. Representative photomicrographs of the distal colon of Rag2-/- mice after T cell transfer. 10 weeks after transfer, mice were euthanized, and colons were harvested, fixed in 10% buffered formalin, and processed for standard H&amp;E staining prior to histological analysis. (i)- (iv) present bright-field micrographs (100x)."
NCBI gene IDs:
"Rag2: 19374"
Figure legend: "E. Expression of FOXP3 protein and mRNA by adoptively transferred Tregs. Mesenteric lymph nodes were excised from the recipients of WT and USP44-/- Tregs in A. FOXP3 protein was detected by intracellular immunostaining and flow cytometry (left). Shown are events within the gate for transferred Tregs (CD4+/CD45.1+). Transferred Tregs were also recovered from mesenteric lymph node cell suspensions by staining for the congenic marker and CD4 followed by FACS, and Foxp3 mRNA was measured in these recovered Tregs by RT-PCR (right)."
NCBI gene IDs:
"Foxp3: 20371
USP44: 327799"
Figure legend: "MC38 colon carcinoma, B16F10 melanoma cell lines were s.c. injected into the shaved flanks of Usp44fl/flFoxp3Cre+ mice and their wild type (Usp44fl/flFoxp3Cre-) littermates (1x105 cells/mouse; n=5-9 mice/group). Tumor dimensions were measured every 2 days, and changes in the mean tumor volume over time were observed (lower panels). Upper panels depict representative photographs of excised tumors."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Usp44: 327799"
Figure legend: "EL4 thymoma cell lines were s.c. injected into the shaved flanks of Usp44fl/flFoxp3Cre+ mice and their wild type (Usp44fl/flFoxp3Cre-) littermates (1x105 cells/mouse; n=5-9 mice/group). Tumor dimensions were measured every 2 days, and changes in the mean tumor volume over time were observed (lower panels). Upper panels depict representative photographs of excised tumors."
NCBI gene IDs:
"Cre: 2777477
Foxp3: 20371
Usp44: 327799"
Figure legend: "(A) Hep3B hepatocytes were treated with nontargeting control siRNA (siNT) or siRNA targeting Dyn2 (siDyn2), followed by re-expression of either vector alone, or GFP-tagged versions of Dyn2, loaded with 150 µM oleate overnight and starved for 48 h in medium containing 0.1% FBS. LDs were visualized by immunofluorescence using Oil Red O staining. Cell boundaries are outlined and those cells re-expressing GFP, GFP-wtDyn2, or GFP--K44A are denoted with asterisks. Bars, 20 µM. (B) Representative blot showing the efficiency of the Dyn2 knockdown in these cells. (C and D) Quantitation of the average LD number and area (in pixels2) per cell from three independent experiments. The data are represented as mean ± SE. *, P ≤ 0.05; **, P ≤ 0.01. NS, not significant."
NCBI gene IDs:
"Dyn2: 25751
Dyn2: 1785"
Figure legend: "HuH-7 (A) and Hep3B (B) hepatocytes were loaded with 150 µM oleate overnight and starved for 48 h in medium containing 0.1% FBS in the presence of Dyn2 inhibitors or DMSO as indicated. Representative images of inhibitor-treated and control cells (stained with Oil Red O) are shown in A and B together with the quantitation of the average LD area per cell from three independent experiments. Pharmacological inhibitors used were: Dynasore (inhibits Dyn2 GTPase activity), MiTMAB (targets PH domain and interferes with membrane binding), Dynole 34-2 (allosteric GTPase inhibitor), and Dynole 31-2 (negative control for Dynole 34-2). Bars, 20 µM."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(C) Representative images from control and Dyn2 knockout MEFs after an overnight lipid loading with 400 µM Dyn2 for 17 h. Knockout of Dyn2 was induced by treatment with 2 µM 4-hydroxy-tamoxifen for 7 d and was confirmed by immunostaining of endogenous Dyn2 (top row) and by immunoblot (D). Bars, 20 µM. (E and F) Average LD number (E) and area (F) per cell from five independent experiments. All data are represented as mean ± SE. *, P ≤ 0.05; **, P ≤ 0.01."
NCBI gene IDs:
"Dyn2: 13430"
Figure legend: "Hep3B cells treated with either a nontargeting control siRNA (A and B, siNT) or an siRNA targeting human Dyn2 (C and D, siDyn2) were fixed and co-stained with antibodies specific for LAMP1 (red) and LC3 (green). After Dyn2 knockdown, a juxtanuclear aggregation and enlargement of the LAMP1-positive compartment is observed (C and D, arrows). Increased labeling of LC3 is also detectable after knockdown of Dyn2."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(E) Western blotting of Hep3B lysates after a 3-d treatment with either the control or Dyn2-targeted siRNA and further treatment with or without 50 µM leupeptin. Densitometry-based analysis of six independent experiments is shown at the bottom of the figure."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "Dyn2 knockdown results in the formation of enlarged autophagic structures. (A-D) Transmission electron micrographs (TEMs) of oleate-loaded Hep3B hepatocytes treated with nontargeting control (siNT) or Dyn2 (siDyn2) siRNA for 72 h. Bars: (A and B) 2 µm; (A′ and B′) 1 µm; (C and D) 0.5 µm. Insets in A and B show fluorescent micrographs of LAMP1-stained cells (bars, 10 µM). Control cells (A) contain an abundance of small (&lt;1 µm) electron-dense lysosomes and autolysosomes (arrowheads). Under conditions in which Dyn2 expression is suppressed (B-D), far fewer small lysosomes are observed. Instead, the cells are populated by larger autolysosomes (≥1 µm) with aberrant morphologies and containing putative LDs (*). (E) Quantitative measure of autolysosomal size from cells in which Dyn2 was knocked down as compared with control cells. Values represent the fold-increase in the number of autophagic structures of a given diameter (measured in microns) observed over siNT-treated control cells. Data were obtained from a single experiment with n = 6 cells examined by electron microscopy from each condition."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "Hep3B cells treated with either a nontargeting control siRNA (A and B, siNT) or an siRNA targeting human Dyn2 (C and D, siDyn2) were fixed and stained with an antibody specific for LAMP1 after a 24-h starvation period in media containing 0.1% FBS. Control cells displayed normal lysosomal compartments, with LAMP1-stained structures dispersed throughout the cytosol, some containing short reformation tubules (B′). After Dyn2 knockdown, many cells contained enlarged autolysosomes with a noticeable increase in persistent and lengthy reformation tubules emanating from LAMP1-positive compartments (C′ and D′). Bars: (A-D) 20 µM; (A′ and B′) 5 µM; (C′ and D′) 10 µM. (E and F) Measurements of the length (E) and distribution (F) of LAMP1-stained ALR tubules in control (siNT) and siDyn2-treated cells. Data represent the mean derived from measurements of tubules in a minimum of 40 cells for each experimental group over 6 independent experiments. Error bars represent SE; *, P ≤ 0.05; **, P ≤ 0.01."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(G) TEM of siDyn2-treated Hep3B hepatocyte exhibiting enlarged autolysosomal structures with extensive tubulation (arrows). Bar, 2 µm. (G′) Inset showing enlargement of autolysosomal tubule (arrows). Bar, 1 µm. (H) Tubulating autolysosome with engulfed lipid droplet. Bar, 0.5 µm."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "Acute inhibition of Dyn2 reversibly disrupts autophagic lysosomal reformation (ALR) and lysosomal tubule scission. (A-D) Still frames from time-lapse movies of Hep3B cells expressing LAMP1-mCherry. Cells were starved for 2 h in HBSS and subsequently treated for 30 min with either DMSO (A and B) or 40 µM Dynasore (C and D), which induced extensive tubulation of LAMP1-positive compartments. Bars (A-D): 20 µM; (A′-B′) 2 µM; (C′-D′) 10 µM. (E-G) To demonstrate the reversibility of this tubulation, Dynasore-treated cells were washed extensively with drug-free media containing 10% FBS and monitored by time-lapse microscopy for 45 min. Frequently, after drug washout, LAMP1-positive tubules exhibited noticeable varicosities (E and F, arrows; bars, 10 µM) along their length. These sites are suggestive of areas of scission and resumed budding of nascent protolysosomes from the reformation tubules (G; bars, 10 µM). (H) Tubules from cells undergoing drug washout were quantified by tracing their lengths at the beginning and end of these movies. Still frames from a representative movie show tubule content at t = 10 and 45 min after drug washout. Bars, 20 µM. (I) Analysis of five independent movies showed an average decrease in total tubulation of ∼50% after drug washout. Data represent the average relative change in total tubule length between the first and last frames of the time-lapse movies. Error bars represent SE; *, P &lt; 0.05."
NCBI gene IDs:
"Dyn2: 1785"
Figure legend: "(C) Co-immunoprecipitation between VP30 and the indicated PPxPxY containing host factors. Empty expression plasmid (Vector) or FLAG-tagged host protein expression plasmids were co-transfected with an HA-VP30 expression plasmid in HEK293T  cells. An anti-FLAG immunoprecipitation (IP: Anti-FLAG) was performed. Western blots of IP  and whole cell lysates (WCL) are shown. Anti-β-tubulin was used as a loading control for the WCLs."
NCBI gene IDs:
"HA: 
VP30: 911826"
Figure legend: "(D) Cells were transfected with empty vector or HA-VP30 expression plasmid and IPs were performed with anti-HA antibody (IP: Anti-HA). Western blotting with anti-HA tag or antibodies to the indicated host proteins was performed on  IP  and on whole cell lysates (WCL)."
NCBI gene IDs:
"HA: 
VP30: 911826"
Figure legend: "(A) Co-immunoprecipitation experiment demonstrating that NP interferes with VP30-PPxPxY  binding. VP30 was co-expressed with the indicated HA-tagged PPxPxY proteins and either empty vector (-) or increasing amounts of FLAG-tagged  NP expression plasmid.  IPs were performed with anti-HA antibodies followed by Western blots  of IPs or WCL by using anti-HA, anti-FLAG  and anti-VP30  antibodies."
NCBI gene IDs:
"FLAG: 
NP: 911830"
Figure legend: "(C) Co-immunoprecipitations between VP30 and the indicated host proteins upon titration of RBBP6. VP30 was co-expressed with FLAG-tagged PPxPxY containing host proteins and either a 5-fold or 10-fold excess of RBBP6 plasmid. IP was performed using anti-FLAG beads followed by Western blotting using the indicated antibodies."
NCBI gene IDs:
"RBBP6: 5930"
Figure legend: "(D) Minigenome (MG) activity upon titration of plasmids encoding GFP  fused to wildtype or single point mutant-RBBP6 peptides. GFP alone or GFP-RBBP6  peptide plasmids were transfected at 12.5 and 25 ng amounts along with the plasmids of the  MG system. The data represent the mean ± S.D. from one representative experiment in which each transfection condition was performed in triplicate (n=3). The experiment was reproduced in two additional, independent experiments (see Appendix Figure S2). Statistical significance was calculated using ANOVA with Tukey's multiple comparisons test. ****p&lt;0.00005; ***p&lt;0.0005"
NCBI gene IDs:
"RBBP6: 5930"
Figure legend: "(C) MG activity upon knockdown of host genes.  HEK293T cells were transfected with scrambled siRNA or siRNA targeting  RBBP6, hnRNP L, hnRNPUL1 or PEG10. Twenty-four hours post-transfection, cells were transfected with  MG assay  plasmids including VP30 at 12.5 and 25ng doses. Data information: , the data represent the mean ± S.D. from one representative experiment in which each transfection condition was performed in triplicate (n=3). The experiment was reproduced in two additional, independent experiments Statistical significance was calculated using ANOVA with Tukey's multiple comparisons test. ****p&lt;0.00005; ***p&lt;0.0005, **p&lt;0.005, *p&lt;0.05."
NCBI gene IDs:
"hnRNP L: 3191
hnRNPUL1: 11100
PEG10: 23089
RBBP6: 5930"
Figure legend: "(A)  HeLa cells were transfected with scrambled siRNA (SCR si) or siRNAs targeting  hnRNP L, hnRNPUL1  or PEG10, 48 h post-transfection, cells were challenged with EBOV-EGFP at an MOI of 0.5. 24 h post-infection, cells were fixed, stained  for nuclei with Hoechst dye and imaged. Scale bar is 200 µm.The graph (right) depicts relative number of infected cells as determined by calculating the ratio of  number of infected cells to  nuclei  and shown relative to SCR siRNA. The data shows a representative result of two independent experiments with the mean ± S.D. calculated from three independently treated wells. Statistical significance was calculated relative to the values obtained in SCR siRNA-treated cells using ANOVA with Dunnett's multiple comparisons test. ***p&lt;0.0001, ***p&lt;0.001, **p&lt;0.01"
NCBI gene IDs:
"hnRNP L: 3191
hnRNPUL1: 11100
PEG10: 23089"
Figure legend: "(C) Immunoblots to assess VP30 and pVP30 levels in lysates from MG assay upon knockdown of RBBP6, hnRNP L or hnRNPUL1."
NCBI gene IDs:
"hnRNP L: 3191
hnRNPUL1: 11100
RBBP6: 5930"
Figure legend: "(D) Immunoblots to assess the levels of VP30, pVP30  and NP in EBOV infected lysates upon knockdown of NPC1 or  RBBP6. HeLa cells were mock transfected or transfected with siRNA targeting NPC1 or RBBP6. 48 h post-transfection cells were infected with  EBOV  at MOI=0.1. At 24 h post-infection, cells were lysed in TRIzol. Protein was extracted from the TRIzol  reagent and analyzed by Western blotting to determine levels of VP30, pSer29  VP30 and  NP."
NCBI gene IDs:
"NPC1: 4864
RBBP6: 5930"
Figure legend: "(E) Western blots detecting pVP30,  VP30  and NP  levels in EBOV infected HeLa cells upon hnRNP L, hnRNPUL1 or PEG10 knockdown."
NCBI gene IDs:
"hnRNP L: 3191
hnRNPUL1: 11100
PEG10: 23089"
Figure legend: "LPS-primed WT and Vdr-/- BMDMs  were infected with 50 MOI S. Typhimurium  for 2 h. Culture supernatants  (SN) and cell lysates(Lysate) were collected and immunoblotted  with the indicated antibodies（A）."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "LPS-primed WT and Vdr-/- BMDMs  were infected with 50 MOI S. Typhimurium for 2 h. Culture supernatants (SN) were collected and (B-E) IL-1β  secretion(B), IL-18  secretion (C) and TNF-ɑ secretion (D) and LDH  release(E) in supernatants. Data are shown as means ± SEM; determined by Student's t-test; *, P &lt; 0.05; **, P &lt; 0.01 In each panel, data are representative of at least three independent experiments."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "LPS-primed WT and Vdr-/- BMDMs  were infected with 50 MOI S. Typhimurium for 2 h. (F) HBVLV-Vdr  expression in Vdr-/- BMDMs  by lentivirus-mediated transduction primed with LPS  and infected with 50 MOI S. Typhimurium for 2 h, cell lysates, and culture supernatants  (SN) were collected and immunoblotted  with the indicated antibodies."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "(G) LPS-primed WT and Vdr-/- BMDMs  were treated with 1 µg/ml FLIC  (LFn-Flagellin  and PA) or PrgJ  （1 µg/ml LFn- PrgJ  and PA）for 1 h, total mixtures( culture supernatants  and cell lysates) were collected and immunoblotted  with the indicated antibodies."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "(H) LPS-primed WT, Vdr-/-, NLRC4-/- and Vdr-/-NLRC4-/- BMDMs  were treated with FLIC  for 1 h, Culture supernatants  (SN) and cell lysates  were collected and immunoblotted  with the indicated antibodies."
NCBI gene IDs:
"NLRC4: 268973
Vdr: 22337"
Figure legend: "LPS-primed WT, Vdr-/-, NLRC4-/- and Vdr-/-NLRC4-/- BMDMs  were treated with FLIC  for 1 h, Culture supernatants (SN) were collected and (I-L) IL-1β secretion(I),  IL-18 secretion (J) and TNF-ɑ secretion (K) and LDH  release(L) in supernatants. Data are shown as means ± SEM; determined by Student's t-test; *, P &lt; 0.05; **, P &lt; 0.01 In each panel, data are representative of at least three independent experiments."
NCBI gene IDs:
"NLRC4: 268973
Vdr: 22337"
Figure legend: "LPS-primed WT, Vdr-/-, NLRP3-/- and NLRP3-/- Vdr-/- BMDMs  were infected with different MOI (0,10,50) S. Typhimurium for 2 h.Culture supernatants  (SN) and Cell lysates were collected and immunoblotted   with the indicated antibodies(A).ELISA"
NCBI gene IDs:
"NLRP3: 216799
Vdr: 22337"
Figure legend: "LPS-primed WT, Vdr-/-, NLRP3-/- and NLRP3-/- Vdr-/- BMDMs  were infected with different MOI (0,10,50) S. Typhimurium for 2 h.Culture supernatants  (SN) were collected antibodies(A).ELISA  detected  TNF-ɑ(B)andIL-1β (C) in supernatants. Data information:n ≥ 3 biological replicates. Data are presented as the mean ± SEM; determined by Student's t-test ; **, P &lt; 0.01."
NCBI gene IDs:
"NLRP3: 216799
Vdr: 22337"
Figure legend: "LPS-primed WT, Vdr-/-, NLRP3-/- and NLRP3-/- Vdr-/- BMDMs  were infected with S. Typhimurium Bacterial burden was detected in spleens(D), PCF(E), and blood  (F)of WT, Vdr-/-, NLRP3-/-, and NLRP3-/- Vdr-/- mice  24 h after S. Typhimurium infection. Data information:n ≥ 3 biological replicates. Data are presented as the mean ± SEM; determined by Student's t-test ; **, P &lt; 0.01."
NCBI gene IDs:
"NLRP3: 216799
Vdr: 22337"
Figure legend: "A LPS-primed WT and Vdr-/- BMDMs  were infected with  S. Typhimurium at an MOI of 50 for 2 h. Endogenous ASC  specks (arrows) were represented and quantified by immunofluorescence  images. The data show representative results from three independent experiments: scale bar, 20 μm. Data are presented as the mean ± SEM; determined by Student's t-test ; ***,p &lt; 0.001."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "B ASC oligomerization  induced by the indicated stimuli in WT and Vdr  −/− BMDMs  primed with LPS."
NCBI gene IDs:
"Vdr: 22337"
Figure legend: "(B-C) Flag-VDR  was co-expressed with Myc-NLRC4  in HEK293T  cells; proteins were immunoprecipitated  and analyzed by immunoblotting  with indicated antibodies. Whole-cell lysates were shown as the input. Data are representative of three independent experiments."
NCBI gene IDs:
"Flag: 
Myc: 
NLRC4: 58484
VDR: 7421"
Figure legend: "(E) WT or mutant NLRC4  (CARD, NACHT, or LRR (leucine-rich repeat)) and HA-VDR   were expressed in HEK293T  cells, immunoprecipitated, and analyzed by immunoblotting  with indicated antibodies.*no specific band. Data are representative of three independent experiments."
NCBI gene IDs:
"HA: 
NLRC4: 58484
VDR: 7421"
Figure legend: "(F) WT or mutant VDR  (DBD or LBD) and Myc-NLRC4  were expressed in HEK293T cells, immunoprecipitated, and analyzed by immunoblotting  with indicated antibodies. Data are representative of three independent experiments."
NCBI gene IDs:
"Myc: 
NLRC4: 58484
VDR: 7421"
Figure legend: "A-B HEK293T  cells were transfected with Flag-NLRC4, VDR, Myc-FLagellin, and HA-NAIP5  (A) or Flag-NLRC4, VDR, Myc- PrgJ  and HA-NAIP2(B) Samples were immunoprecipitated  with the anti-Flag  and analyzed by immunoblotting  with indicated antibodies."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
FLagellin: 1253480
NAIP2: 17948
NAIP5: 17951
NLRC4: 58484
PrgJ: 1254395
VDR: 7421"
Figure legend: "HEK293T  cells were transfected with Flag-NLRC4, LBD-VDR, Myc-FLagellin, and HA-NAIP5  (C) or Flag-NLRC4, LBD-VDR, Myc-PrgJ, and HA-NAIP2  (D). Samples were immunoprecipitated  with the anti-Flag  and analyzed by immunoblotting  with indicated antibodies."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
FLagellin: 1253480
NAIP2: 17948
NAIP5: 17951
NLRC4: 58484
PrgJ: 1254395
VDR: 7421"
Figure legend: "B VDR  or mutant VDR(K123A)and Myc-NLRC4  were expressed in HEK293T  cells, immunoprecipitated  with the anti-Flag  antibody, and analyzed by immunoblotting  with indicated antibodies."
NCBI gene IDs:
"Myc: 
NLRC4: 58484
VDR: 22337"
Figure legend: "C Transfected VDR  or mutant VDR(K123A) and Flag-NLRC4, Myc- PrgJ, and HA-NAIP2  in HEK293T  cells, immunoprecipitated  with the anti-HA  antibody, and analyzed by immunoblotting  with indicated antibodies."
NCBI gene IDs:
"Flag: 
HA: 
Myc: 
NAIP2: 17948
NLRC4: 58484
PrgJ: 1254395
VDR: 22337"
Figure legend: "D Vdr-/- BMDMs  were infected with lentivirus, which expressed Vector(NC), VDR, and VDR-K123A, infected with  S. Typhimurium  at an MOI of 50 for 2 h, cell lysates(Lysate) and culture supernatants(SN) were collected and immunoblotted  with the indicated antibodies."
NCBI gene IDs:
"Vdr: 22337
VDR: 7421"
Figure legend: "(H) HA-tagged EBNA2 candidate phosphorylation mutants were expressed in DG75 B cells and tested for activation of an EBNA2/CBF1 responsive promoter reporter luciferase plasmid. Activation of the reporter gene is shown as relative response ratio normalized to Renilla luciferase activity and shown relative to wt activity."
NCBI gene IDs:
"HA: 
luciferase: 
CBF1: 9541
EBNA2: 3783761"
Figure legend: "(A) Flag-tagged active (PLK1) and kinase inactive PLK1 (K82M) were co-expressed with HA-EBNA2 wt, docking site mutant (S379A) and phosphorylation mutant (S457A/T465V) and tested for activation of an EBNA2/CBF1 responsive promoter reporter in DG75 cells. Activation of the reporter firefly luciferase gene is shown as relative response ratio normalized to Renilla luciferase activity and shown relative to EBNA2 wt activity."
NCBI gene IDs:
"Flag: 
HA: 
luciferase: 
CBF1: 9541
EBNA2: 3783761
PLK1: 5347"