EMBO/SourceData huggingface dataset:
Figure legend: "Differentially expressed (DE) exons and introns in NRPB2Y732F +/+ nrpb2-2 -/- compared to NRPB2WT +/+ nrpb2-2 -/- based on RNA-seq results. Numbers of DE exons and introns were shown in plot. Quantification (log fold change of FPKM from RNA-seq) of differentially expressed (DE) exons and non-DE exons in NRPB2Y732F +/+ nrpb2-2 -/- compared to NRPB2WT +/+ nrpb2-2 -/-. ** denotes p-value &lt;0.01 by Wilcoxon signed-rank test. The solid horizontal lines and box limits represent medians, lower and upper quartiles of data values in each group. The upper and lower whiskers extend to the largest or smallest value, respectively, no further than 1.5 * IQR from the relevant quartile."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "Genome browser snapshots illustrating enhanced exon skipping in NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F, red) compared to NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT ,blue). Scale bars denote 0.5 Kb. Quantification (log fold change of FPKM from RNA-seq) of differentially expressed (DE) introns and non-DE exons in NRPB2Y732F +/+ nrpb2-2 -/- compared to NRPB2WT +/+ nrpb2-2 -/-. **** denotes p-value &lt;0.0001 by Wilcoxon signed-rank test. The solid horizontal lines and box limits represent medians, lower and upper quartiles of data values in each group. The upper and lower whiskers extend to the largest or smallest value, respectively, no further than 1.5 * IQR from the relevant quartile."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "plaNET-seq signal of RNAPII at 3' end of AT2G21410 in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red). Arrows indicate the RNAPII signal peaks at PAS stalling region."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259
AT2G21410: 816680"
Figure legend: "Metagene profile of plaNET-seq mean signal of RNAPII in a 1 kb window centered at PAS (n=24448) in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red). The significance of differences of plaNET-seq signal in the region from PAS to +100 bins between NRPB2WT and NRPB2Y732F were assessed by Two-sided Mann-Whitney U-test, p = 1.53e-06."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "Histogram of transcriptional read-through length (nt) from PAS of protein-coding gene (plaNET-seq FPKM&gt;5, n=9316) in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red)."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "Box plot shows the RNAPII transcriptional read-through length from PAS of protein-coding genes (plaNET-seq FPKM&gt;5 n=9316) called based on statistic model (see Methods) in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red). Median of read-through length in NRPB2WT and NRPB2Y732F mutant are 534 nt and 649 nt. Two-sided Mann-Whitney U-test: *** denotes p = 9.9e-62. The solid horizontal lines and box limits represent medians, lower and upper quartiles of data values in each group. The upper and lower whiskers extend to the largest or smallest value, respectively, no further than 1.5 * IQR from the relevant quartile."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "Metagene plot of RNAPII signal by plaNET-seq anchored at both PAS of upstream genes and TSS of downstream genes for tandemly oriented genes (n=5753) in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red). Red arrow denotes the direction of transcriptional read-through. Pink dashed line rectangle indicates the region corresponding to the second half of PAS-TSS gaps along 5' to 3' direction. Metagene plot of RNAPII signal by plaNET-seq anchored at PASs of both upstream genes and downstream genes for gene pairs located in \"tail to tail\" orientation (n=1384) in NRPB2WT +/+ nrpb2-2 -/- (NRPB2WT, blue) and NRPB2Y732F +/+ nrpb2-2 -/- (NRPB2Y732F ,red). Red arrows denote the directions of transcriptional read-through from both PASs. Pink dashed line rectangles indicate the region corresponding to the second half of PAS-TSS gaps along 5' to 3' direction."
NCBI gene IDs:
"NRPB2: 828259
nrpb2: 828259"
Figure legend: "B. MASTL  was ablated in MDA-MB-231  cells using the isgMASTL system. 72 h after Dox, cells were starved  of glucose  in the media in presence of 10% dFBS for 1 h (-) and then  re-stimulated with 25 mM of glucose  for 10 min before recovery (+). Whole-cell lysates were blotted  with the indicated antibodies. * in indicates unspecific band. β-Actin  was used as a loading control."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "C. MASTL  was depleted in MDA-MB-231 cells using shRNAs against MASTL  (+) or scrambled shRNAs (−) as control. Cells were starved  from glucose  for 2 h and re-stimulated  with 5 mM glucose  for 15 min and 1 h. Total extracts were recovered and tested for the indicated antibodies. β-Actin was used as a loading control. D. Quantification of phospho-AKT  T308 levels in glucose deprivation  or glucose stimulation  (15 min) conditions in MDA-MB-231 control cells and MASTL-depleted cells either using shRNA or the inducible sgRNA. Bars displays mean data + SEM from three independent experiments. Significance determined by Student's t-test comparing feedback activation in MASTL-depleted cells versus control cells (ns, not significant; *P&lt;0.05)."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "E. Immunoblot  analysis with the indicated antibodies in BT-549  cells upon glucose deprivation  and 15 min glucose stimulation. MASTL  knockdown was performed by infection with a shRNA for MASTL  (+) or a Scramble shRNA (−) as a control.  β-Actin was used as a loading control. Bar chart displays mean data + SEM from 3 experiments. Significance determined by Student's t-test comparing feedback activation in MASTL-depleted cells versus control cells (ns, not significant). F. Immunoblot  analysis with the indicated antibodies in MCF-7 cells upon glucose deprivation  and glucose stimulation  for 15 min. MASTL  knockdown was performed by infection with a shRNA for MASTL  (+) or a Scramble shRNA (−) as a control. Bar chart displays mean data + SEM from 3 experiments. Significance determined by Student's t-test comparing feedback activation in MASTL-depleted cells versus control cells (ns, not significant; *P&lt;0.05)."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "A. Time course  experiment at the indicated  time points  after 100 nM insulin  stimulation in 6 h serum-starved  control and MASTL  knockout MDA-MB-231 cells using the isgMASTL system. The plot shows the quantification of phospho-AKT  T308 after 30 min of insulin stimulation. Plots displays mean data + SEM from 5 independent experiments. *P&lt;0.05, Student's t-test."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "B. TSC2  was knocked down in control or MASTL-null MDA-MB-231 cells using specific (+) or Scrambled (−) shRNAs. Cells were starved for glucose  for 1 h. The plot shows the quantification of phospho-AKT  T308. Data are mean + SEM from 3 independent experiments. ns, not significant; *P&lt;0.05, 1-way ANOVA."
NCBI gene IDs:
"MASTL: 84930
TSC2: 7249"
Figure legend: "C. MDA-MB-231  control (−) or MASTL  null (+) cells were treated with 100 nM rapamycin  or vehicle as control. Cells were starved  for glucose  for 1 h and  re-stimulated  with glucose  for 15 min; rapamycin  was added 15 min before glucose stimulation. The plot shows the quantification of phospho-AKT  T308. Data are mean + SEM from 3 independent experiments. ns, not significant; *P&lt;0.05, 1-way ANOVA."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "E. GLUT4 staining  (magenta) in skeletal muscle sections. Vinculin  (membrane) and DAPI  (DNA) staining  is shown in yellow and cyan, respectively. Scale bars, 50 µm. Mice  were fasted  overnight for 16 h, and  re-fed  for 2 h before sample collection. Quantification of the mean intensity [relative units x103; n=3 Mastl(+/+) and 3 Mastl(∆/∆)] is shown. Data points reflect the mean + SEM; *P&lt;0.05; ***P &lt;0.001; unpaired Student's t-test."
NCBI gene IDs:
"Mastl: 67121"
Figure legend: "G. Immunoblot  with the indicated antibodies in liver  tissues from Mastl(+/+) (n=6) and Mastl(Δ/Δ) (n=7) mice. Mice  were fasted overnight for 16 h, injected intraperitoneally with glucose  (2 g/kg body), and sacrificed 30 min later for sample collection. Quantification of the relative fold change signal of phospho-AKT  T308 (normalized to total AKT  level). n=6 Mastl(+/+) and 7 Mastl(Δ/Δ) mice. Charts reflect the mean + SEM; *P&lt;0.05; unpaired Student's t-test."
NCBI gene IDs:
"Mastl: 67121"
Figure legend: "A. Co-depletion of ENSA and ARPP19  proteins in the MDA-MB-231 cell line using specific siRNAs or control siRNAs against luciferase  (Luc). 72 h after transfection cells were starved  for glucose  for 1 h and re-stimulated  with glucose  for 15 min. Total cell extracts were collected and analyzed for the indicated antibodies."
NCBI gene IDs:
"Luc: 
luciferase: 
ARPP19: 10776
ENSA: 2029"
Figure legend: "B. isgMASTL  cells were previously infected with lentiviral  supernatants expressing FLAG- and HA-tagged versions of ENSA  and ARPP19  phospho-mimetic mutants (S67D and S62D) or GFP  as a control. Cells were treated with Dox  to induce MASTL CRISPR/Cas9-dependent knock-out, and 72 h later cells were treated as specified in A. The plot displays mean + SEM from 3 independent experiments. Significance determined by Student's t-test comparing ENSA/ARPP19  overexpression versus control cells (ns, not significant; *P&lt;0.05)."
NCBI gene IDs:
"CRISPR: 
FLAG: 
GFP: 
HA: 
ARPP19: 10776
Cas9: 69900935
ENSA: 2029
MASTL: 84930"
Figure legend: "C. Control (Dox  −) and MASTL  knockout (Dox  +) cells were treated with 5 µM fostriecin, 50 nM  okadaic acid (OA) or vehicle (Veh) 10 min before insulin  stimulation for 30 min. Total lysates were analyzed for the indicated antibodies and P-T308-AKT  signal was quantified. β-Actin was used as a loading control. The plot represents mean + SEM from 3 independent experiments, except for fostriecin  treatment in which only data from two experiments were obtained and no error bars are shown. *P&lt;0.05; Student's t-test."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "A. MDA-MB-231 cells were co-transduced with specific siRNAs against the PPP2R2A  (B55α) and PPP2R2D  (B55δ) transcripts (B55αδ) or against luciferase  (Luc) as a control. Asynchronous cells were collected and blotted  for the indicated antibodies. The histograms represent the quantifications of the specified phospho-residues. Plots show mean + SEM from 3 independent experiments. *P&lt;0.05, Student's t-test."
NCBI gene IDs:
"Luc: 
luciferase: 
PPP2R2A: 5520
B55α: 5520
PPP2R2D: 55844
B55δ: 55844"
Figure legend: "B. Immunodetection  of the indicated antigens in control (−) and MASTL  knockout (+) cells starved  from glucose  and re-stimulated with glucose  for 15 min."
NCBI gene IDs:
"MASTL: 84930"
Figure legend: "C. siRNA-mediated genetic depletion of B55alpha  and delta subunits (B55αδ) in control (Dox  −) and MASTL-null (Dox  +) cells. The phosphorylation  status of the different proteins was scored in conditions of glucose  re-addition. n=3 independent experiments. The plot shows mean + SEM from 3 independent experiments. *P&lt;0.05, 1-way ANOVA."
NCBI gene IDs:
"MASTL: 84930
B55alpha: 5520
B55α: 5520"
Figure legend: "D. Immunoblot  with the indicated antibodies in  skeletal muscle (gastrocnemius) extracts. Mice  were fasted overnight for 16 h, injected intraperitoneally with glucose  (2 g/kg body), and sacrificed 30 min later for sample collection. Quantification of the relative fold change signal of total IRS1, and phospho-S6K1  T389 (normalized to total S6K1  level; n=6 Mastl(+/+) and 7 Mastl(∆/∆)). vinculin  were used as a loading control. Data are mean + SEM; *P&lt;0.05, unpaired Student's t-test."
NCBI gene IDs:
"Mastl: 67121"
Figure legend: "B. Cas9-expressing control (−) cells or cells with CRIPSR/Cas9-mediated TSC2  knockout (sgTSC2) were treated with rapamycin  (Rap), Torin1  (Tor), or vehicle as control (V). MASTL-knockdown cells (shMASTL) were used as an additional control of the specificity of the antibody. Inhibitors were added 15 min before glucose  re-stimulation for additional 15 min and total cell lysates were harvested for immunoblot. The plot shows the quantification of phospho-MASTL  T194 as mean from 2 independent experiments."
NCBI gene IDs:
"CRIPSR: 
Cas9: 69900935
MASTL: 84930
TSC2: 7249"
Figure legend: "G. isgMASTL MDA-MB-231 cells were transduced with the indicated Mastl  mutants or GFP  alone as a control, and treated with doxycycline  (Dox) to induce Mastl  deletion. Cells were collected after glucose  stimulation, and total lysates blotted  for the indicated antibodies."
NCBI gene IDs:
"GFP: 
MASTL: 84930
Mastl: 67121"
Figure legend: "(G) GTT or ITT in 17-week-old wild-type or JFKTG mice fed on HFD. Error bars represent mean ± SEM (n = 6, *p &lt; 0.05, paired two-tailed Student's t-test)."
NCBI gene IDs:
"JFK: 54455"
Figure legend: "(H) H&amp;E and Oil Red O staining of liver sections from wild-type or JFKTG mice fed on ND or HFD. Scale bar, 100 μm."
NCBI gene IDs:
"JFK: 54455"
Figure legend: "(D) The body weight of male wild-type or JfkKO mice fed on HFD. Error bars represent mean ± SEM (n = 6, *p &lt; 0.05, paired two-tailed Student's t-test)."
NCBI gene IDs:
"Jfk: 54455"
Figure legend: "(G) H&amp;E and Oil Red O staining of liver sections from wild-type or JfkKO mice fed on HFD. Scale bar, 100 μm."
NCBI gene IDs:
"Jfk: 54455"
Figure legend: "(H) Wild-type or JfkKO mice were fed with HFD for 11 weeks prior to injection of adenovirally-delivered vector or JFK via tail vein. Seven days after injection, total proteins prepared from liver tissues were subjected to western blotting analysis for the expression of Jfk or measurement for hepatic TG and NEFA content. Error bars represent mean ± SEM (n = 6, *p &lt; 0.05, one-way ANOVA with Tukey's HSD test). H&amp;E and Oil Red O staining of liver sections are shown. Scale bar, 100 μm."
NCBI gene IDs:
"Jfk: 54455
JFK: 54455"
Figure legend: "(A) HepG2 cells were stably transfected with vector or FLAG-JFK. Cellular extracts were immunopurified with anti-FLAG immunoafﬁnity resin and eluted with FLAG peptides. The eluates were resolved on SDS-PAGE and silver-stained. The proteins bands were retrieved and analyzed by mass spectrometry."
NCBI gene IDs:
"FLAG: 
JFK: 54455"
Figure legend: "(E) HepG2 cells were transfected with FLAG-JFK and treated with 50 μg/ml CHX for the indicated times in the presence or absence of MG132 for western blotting analysis. Quantitation was done by densitometry and expressed as signals of ING5/β-actin."
NCBI gene IDs:
"FLAG: 
JFK: 54455"
Figure legend: "(C) HepG2 cells were treated with control siRNAs or siRNAs against JFK or/and ING5, or transfected with vector, JFK, or/and ING5. Cellular extracts were prepared for western blotting analysis with the indicated antibodies."
NCBI gene IDs:
"JFK: 54455
ING5: 84289"
Figure legend: "(D) HepG2 cells were transfected with vector or JFK, AMPKα1, or/and ING5, or treated with control siRNAs or siRNAs against JFK, AMPKα1, or/and ING5, Cellular extracts were prepared for AMPK activity assays by ELISA. Error bars represent mean ± SD for triplicate experiments (*p &lt; 0.05, paired two-tailed Student's t-test)."
NCBI gene IDs:
"JFK: 54455
ING5: 84289
AMPKα1: 5562"
Figure legend: "(D) The measurement of AMPK activity and the rate of fatty acid β-oxidation of wild-type or JfkKO mice. Cellular extracts prepared from MEFs or liver tissues were subjected to AMPK activity assays by ELISA. Mitochondria were isolated from MEFs or liver tissues, and the rate of fatty acid β-oxidation was quantified by spectrophotometric detection for ferricyanide-trapped reduced products. Error bars represent mean ± SEM (n = 6, *p &lt; 0.05, paired two-tailed Student's t-test)."
NCBI gene IDs:
"Jfk: 54455"
Figure legend: "Wild-type or JfkKO mice were fed with HFD for 11 weeks prior to injection of adenovirally-delivered vector control, shIng5, or shAmpkα1 via tail vein. Seven days after injection, cellular extracts prepared from liver tissues were subjected to western blotting analysis or measurement for hepatic TG and NEFA content (E). Asterisk (*) represents significant comparison to wild-type mice, octothorpe (#) represents significant comparison to JfkKO mice. Error bars represent mean ± SEM (n = 6, *p &lt; 0.05, #p &lt; 0.05, one-way ANOVA with Tukey's HSD test)."
NCBI gene IDs:
"Jfk: 54455
Ing5: 66262
Ampkα1: 105787"
Figure legend: "B. Characteristics of 96 patients with an EGFR-activating mutation who were treated with EGFR-TKI. Each column corresponds to one patient. Data include general characteristics (stage at diagnosis, age, sex, tobacco usage, position of EGFR mutation, TKI type), progression-free survival (PFS; lower graph) and intensity of RHOB staining as determined by immunohistochemistry (null: 0, weak: +, moderate: ++ and high: +++)."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "C. Representative scans of patients with low or high RHOB-expressing lung tumors, before and after erlotinib treatment. Red arrows indicate lung tumors."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "D. Progression-free survival of erlotinib-treated patients with EGFR-mutated lung tumors, according to RHOB expression, assessed by immunohistochemistry (low RHOB group = negative + weak staining; high RHOB group = moderate + high staining)."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "G. RHOB immunostaining score evolution in EGFR-mutated lung tumors before treatment and after EGFR-TKI relapse."
NCBI gene IDs:
"EGFR: 1956"
Figure legend: "A. Representative H&amp;amp;amp;E staining of whole lungs from EGFRL858R/Rhob-/-, EGFRL858R/Rhob+/- and EGFRL858R/Rhob+/+ mice treated or not with erlotinib (12.5 mg/kg/day) for four days. Scale bar: 5 mm. B. Quantification of the tumor/lung ratio. n=7 for each group except for EGFRL858R/Rhob+/- placebo (n=6)."
NCBI gene IDs:
"EGFR: 13649
Rhob: 11852"
Figure legend: "C. Representative Ki67 immunostaining of EGFRL858R/Rhob-/-, EGFRL858R/Rhob+/- and EGFRL858R/Rhob+/+ mice treated or not with erlotinib (12.5 mg/kg/day) for four days (scale bar: 50 µm), and the corresponding quantification (D). Three independent zones/mice lung were used for quantification. n=21 (7 mice) for each group except for EGFRL858R/Rhob+/- placebo (n=18; 6 mice)."
NCBI gene IDs:
"EGFR: 13649
Rhob: 11852"
Figure legend: "E. Immunostaining of cleaved caspase-3 in lung tumors from EGFRL858R/Rhob+/+ or EGFRL858R/Rhob-/- mice treated for 24 h with erlotinib at 12.5 mg/kg. Scale bar: 50 µm. (**: p&lt;0.001 vs. placebo; ***: p&lt;0.0001 vs. placebo)."
NCBI gene IDs:
"EGFR: 13649
Rhob: 11852"
Figure legend: "HCC4006 cells were (A) transfected with two siRNA against RHOB (siB1, siB2) then treated with increasing doses of erlotinib. The surviving cell fraction was determined by an MTS assay after 72 h and compared to untreated cells."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "HCC4006 cells were (A) transfected with two siRNA against RHOB (siB1, siB2). RHOB overexpression was monitored by western blotting. (***: p&lt;0.0001 vs. control cells). Data are representative of at least three independent experiments."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "HCC4006 cells were (B) transduced with control (AdCont) or RHOB-overexpressing adenoviruses (AdRHOB) then treated with increasing doses of erlotinib. The surviving cell fraction was determined by an MTS assay after 72 h and compared to untreated cells."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "HCC4006 cells were (B) transduced with control (AdCont) or RHOB-overexpressing adenoviruses (AdRHOB). RHOB overexpression was monitored by western blotting. (***: p&lt;0.0001 vs. control cells). Data are representative of at least three independent experiments."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "Erlotinib IC50 values were quantified in RHOB-overexpressing or RHOB-depleted (D) HCC827 cells, (E) HCC2935 cells and (F) H3255 cells, as determined by an MTS assay after 72 h treatment. RHOB overexpression or inhibition was monitored by western blotting for each condition."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "G. HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing adenoviruses (AdRHOB) at an increasing multiplicity of infection (MOI), then erlotinib IC50 values were determined after 72 h by an MTS assay and a correlation analysis was performed."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "G. HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing adenoviruses (AdRHOB) at an increasing multiplicity of infection (MOI). RHOB overexpression was monitored by western blotting. (***: p&lt;0.0001 vs. control cells). Data are representative of at least three independent experiments."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "A. HCC4006, HCC827, HCC2935 and H3255 cells were transduced with control (AdCont) or RHOB-overexpressing (AdRHOB) adenoviruses and treated for four hours with erlotinib at concentrations corresponding to the respective IC50 values determined for each control cell line. The phosphorylation status of AKT, ERK1/2 and EGFR was assessed by western blotting and normalized according to total protein levels. RHOB overexpression was also monitored by western blotting."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "B. Representative immunostaining of phospho-AKT (Ser473), phospho ERK1/2 and their total protein amounts in lung tumors from EGFRL858R/Rhob-/- or EGFRL858R/Rhob+/+ mice treated or not with erlotinib (12.5 mg/kg/day) for four days. The remaining hyperplastic areas were selected in erlotinib-treated mice to efficiently characterize the effect of erlotinib on ERK and AKT pathways in both Rhob genotypes. Scale bar: 100 µm."
NCBI gene IDs:
"EGFR: 13649
Rhob: 11852"
Figure legend: "C. HCC4006 cells were transfected with a plasmid coding for a constitutively active AKT mutant (AKTmyr, myristoylated) or an empty vector (ø), and treated for 72 h with increasing concentrations of erlotinib. The surviving cell fraction was determined by an MTS assay. Data are representative of at least three independent experiments."
NCBI gene IDs:
"AKT: 10000///208///207"
Figure legend: "C. HCC4006 cells were transfected with a plasmid coding for a constitutively active AKT mutant (AKTmyr, myristoylated) or an empty vector (ø), and treated for 72 h with increasing concentrations of erlotinib. AKT overexpression and phosphorylation at Ser473 were assessed by western blotting. Data are representative of at least three independent experiments."
NCBI gene IDs:
"AKT: 10000///208///207"
Figure legend: "A. HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing (AdRHOB) adenoviruses and treated for four hours with erlotinib (100 nM), G594 (100 nM), or a combination of both drugs. The phosphorylation status of GSK3β (Ser9), ERK1/2 and EGFR (Tyr1173) was assessed by western blotting and normalized according to the total protein levels. RHOB overexpression was also monitored by western blotting."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "B. HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing (AdRHOB) adenoviruses and treated for 72 h with erlotinib alone (black and red curves) or in combination with the AKT inhibitor G594 at 100 nM (green and blue curves). The surviving cell fraction was determined by an MTS assay."
NCBI gene IDs:
"RHOB: 388"
Figure legend: "C. HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing adenoviruses (AdRHOB) and treated for 72 h with increasing concentrations of erlotinib in the absence or presence of increasing doses of G594. The surviving cell fraction was determined by an MTS assay, and erlotinib IC50 values were determined for each condition. (**: p&lt;0.001 vs. AdCont cells; ***: p&lt;0.0001 vs. AdCont cells). HCC4006 cells were transduced with control (AdCont) or RHOB-overexpressing (AdRHOB) adenoviruses and treated for 48 h with erlotinib (100 nM), G594 (100 nM), or a combination of both drugs."
NCBI gene IDs:
"RHOB: 388"