EMBO/SourceData huggingface dataset:
Figure legend: "F Basal and stimulated (+200 nM isoproterenol) lipolysis in isolated adipocytes from 10-week-old WT and FADD-D mice (n = 4 for each genotype) treated with or without 10 μM BAY. Data are expressed as mean ± SEM. *P = 0.0033, **P = 0.0051, ^P = 0.004, ^^P = 0.0057 (one-way ANOVA). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A Typical growth curves of control and ad-FADDmice maintained on a SD (left panel) or a HFD (right panel) (n = 8 for each genotype). Data are expressed as mean ± SEM. *P = 0.0435, **P =0.0371, ^P = 0.0217, #P = 0.0236, ##P = 0.0158, ###P = 0.0113 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "B Body composition by NMR to show the fat mass and fat mass ratio in control and ad-FADDmice after 10 weeks of HFD (n = 6 for each genotype). Data are expressed as mean ± SEM. *P = 0.0027, **P = 0.0012 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "C Food intake in 15-week-old male control and ad-FADDmice fed a HFD (n = 8 for each genotype)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "D and E Representative pictures of hematoxylin and eosin-stained sections of epididymalWAT from control and ad-FADDmice at 10 weeks (D) and after 10 weeks of an HFD (E) are shown. Scale bar = 50 μm. Shown are typical results from four different fields and three different experiments.F The average cross-sectional area of adipocytes in epididymalWAT is shown in the bar graph (n = 6 for each genotype). Data are expressed as mean ± SEM. *P = 0.002 (Student's t-test). NS, not statistically significant.G The distribution of adipocyte cross-sectional area in epididymalWAT of HFD-fed control or ad-FADDmice is shown in the bar graph (n = 6 for each genotype)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "H Representative pictures of hematoxylin and eosin-stained sections of brown adipose tissue from HFD-fed control and ad-FADDmice are shown. Scale bar = 50 μm. Shown are typical results from four different fields and three different experiments."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A-C HFD-fed control and ad-FADDmice were housed in a computer-controlled open-circuit indirect calorimeter to determine (A) oxygen consumption, (B) carbon dioxide production, and (C) respiratory exchange ratio (RER) during the light (8 am - 8 pm) and dark (8 pm - 8 am) periods (n = 5 for each genotype). Data are expressed as mean ± SEM. *P = 0.0379, **P = 0.0453, ***P = 0.0445 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "D The result of real-time quantitative PCR analysis for UCP1 in brown adipose tissue from HFD-fed control and ad-FADDmice is shown in the bar graph. Data are expressed as mean ± SEM from two independent experiments (n = 4 total for each genotype). *P = 0.0172 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082
UCP1: 22227"
Figure legend: "E Immunoblot of UCP1 and Tubulin (control) (top) in BAT from control and ad-FADDmice with relative quantification (bottom). Data are expressed as mean ± SEM from three independent experiments. *P = 0.0053 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "D Insulin-stimulated Akt phosphorylation (Ser473) in WAT, liver, and skeletal muscle of control and ad-FADD mice. The experiments were performed in triplicate."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "E Adiponectin mRNA was measured in isolated epididymal adipocytes by RT-PCR. Data are expressed as mean ± SEM from three independent experiments. *P = 0.0044 (Student's t-test)."
NCBI gene IDs:
"Adiponectin: 11450"
Figure legend: "I Basal and insulin-stimulated glucose uptake in control and FADD deficient primary isolated adipocytes. Data shown are from one experiment (n = 4 for each genotype), representative of a total of two independent experiments. Results are means ± SEM. *P = 0.0073 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "J Basal and insulin-stimulated glucose uptake in 3T3L1 cells differentiated to adipocytes and infected with lentivirus containing FADD shRNA or scrambled shRNA control. Data are expressed as mean ± SEM from three independent experiments. *P = 0.0092 (Student's t-test)."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "K Basal and insulin-stimulated glucose uptake in control and FADD deficient primary isolated adipocytes treated with DMSO or 5 μM MK886. Data are expressed as mean ± SEM from four independent experiments. *P = 0.0135 (one-way ANOVA). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082"
Figure legend: "A Relative mRNA levels of the macrophage marker F4/80 in SVCs isolated from epididymal adipose tissue. Data are expressed as mean ± SEM from four independent experiments. *P = 0.0279 (Student's t-test)."
NCBI gene IDs:
"F4/80: 13733"
Figure legend: "C Relative mRNA levels of the proinflammatory M1-like macrophage marker CD11c in SVCs isolated from epididymal adipose tissue. Data are expressed as mean ± SEM from five independent experiments. *P = 0.0221 (Student's t-test)."
NCBI gene IDs:
"CD11c: 16411"
Figure legend: "A Top, representative photographs of 20-week-old male ob/ob and FADD-D/ob/ob mice fed a SD. Bottom, representative photographs of their livers and epididymalWAT."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "B Comparison of weights of WAT depots from WT, FADD-D, ob/ob and FADD-D/ob/ob mice. n = 5 for each genotype. Data are expressed as mean ± SEM. *P = 0.0007, **P = 0.0022, ***P = 0.0011, #P = 0.0009, ##P = 0.0003, ###P = 0.0027 (one-way ANOVA)."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "C Basal and stimulated lipolysis measured by fatty acid release from explants of epididymalWAT in 10-week-old male ob/ob and FADD-D/ob/ob mice fed a HFD. n = 4 for each genotype. Data are expressed as mean ± SEM. *P = 0.0003, **P = 0.001 (one-way ANOVA)."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "D cAMP level in WAT of 10-week-old male WT, FADD-D, ob/ob and FADD-D/ob/ob mice fed a HFD. n = 4 for each genotype. Data are expressed as mean ± SEM. *P = 0.0017, **P = 0.001 (one-way ANOVA)."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "E Whole-body oxygen consumption rate (VO2) during 24 hr in WT, FADD-D, ob/ob, and FADD-D/ob/ob mice fed a SD. n = 4 for each genotype. Data are expressed as mean ± SEM. *P = 0.0053, **P = 0.0025, ***P = 0.0062, ****P = 0.0011 (one-way ANOVA)."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "F Quantitative real time PCR for Dio2, PPARd and UCP1, using RNA from epididymal fat from 20-week-old male ob/ob and FADD-D/ob/ob mice fed a SD. Data are expressed as mean ± SEM from four independent experiments. *P = 0.0009, **P = 0.0015, ***P = 0.0003 (Student's t-test)."
NCBI gene IDs:
"Dio2: 13371
FADD: 14082
ob: 16846
PPARd: 19015
UCP1: 22227"
Figure legend: "G Quantitative real time PCR for UCP3, PGC-1α, PPARd and CPT1, using RNA from skeletal muscle from 20-week-old male ob/ob and FADD-D/ob/ob mice fed a SD. Data are expressed as mean ± SEM from four independent experiments. *P = 0.0105, **P = 0.0033, ***P = 0.0058 (Student's t-test). NS, not statistically significant."
NCBI gene IDs:
"CPT1: 12895///12894
FADD: 14082
ob: 16846
PPARd: 19015
PGC-1α: 10891
UCP3: 22229"
Figure legend: "H Quantitative real time PCR for UCP1, PPARa, CPT1 and PGC-1α, using RNA from brown adipose fat from 20-week-old male ob/ob and FADD-D/ob/ob mice fed a SD. Data are expressed as mean ± SEM from four independent experiments. *P = 0.0012 **P = 0.0129, ***P = 0.0103, ****P = 0.0207 (Student's t-test)."
NCBI gene IDs:
"CPT1: 12895///12894
FADD: 14082
ob: 16846
PPARa: 19013
PGC-1α: 19017
UCP1: 22227"
Figure legend: "I Oxidation of [1-14C] oleic acid to 14CO2by adipocytes isolated from WT, FADD-D, ob/ob, and FADD-D/ob/ob mice. Data shown are from one experiment (n = 3 for each genotype), representative of a total of two independent experiments. Results are means ± SEM. *P = 0.0031, **P = 0.0075 (one-way ANOVA). NS, not statistically significant."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "J Immunoblot of VLCAD, LCAD, MCAD, PTL and Tubulin (control), using protein extracts from epididymal fat from 15-week-old male WT, FADD-D, ob/ob and FADD-D/ob/ob mice fed a SD. Data shown are representative of three independent experiments having similar results."
NCBI gene IDs:
"FADD: 14082
ob: 16846"
Figure legend: "(B) Epg5 protein and the wild-type copy of Epg5 are absent in Epg5−/− mice. There is an intervening lane between Epg5+/− and Epg5−/− lanes in the Western blot."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(C) Body weight curves of Epg5+/− and Epg5−/− males. Means ± SEM of 12 mice are shown."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(E) Epg5−/− males show gradually worsening limb-clasping reflexes."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(F) The hindquarters of Epg5−/− mice are completely paralyzed at 10 mo of age."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(G) Spinal deformation in an Epg5−/− male at 10 mo of age."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(H) Epg5−/− females gradually develop the limb-clasping phenotype."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(A) Nissl staining of the fifth layer of the cortex in 10-mo-old Epg5+/− and Epg5−/− mice. (B) The number of pyramidal cells in the fifth layer per millimeters squared."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(C and D) H&amp;amp;amp;E staining of the hippocampus shows reduced pyramidal cell numbers in Epg5−/− mice. Means ± SEM of three mice are shown in B and D."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(E) The normally developed cerebellum in Epg5−/− mice. (F) The thickness of the molecular layer in the cerebellum in mutant and control mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(G and H) Purkinje cells, stained by calbindin, are not reduced in number in Epg5−/− mice. Means ± SEM of five mice are shown in F and H."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(I) Arrows indicate Pyknotic neurons in the anterior horn of Epg5−/−mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(J) The number of motor neurons in the spinal cord (SC) in Epg5+/− and Epg5−/−mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(K) The numbers of Nissl-stained interneurons in the spinal cord are similar in control and Epg5−/− mice. Means ± SEM of three mice are shown in J and K."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(L) Eosinophilic spheroids (arrows) accumulate in the thoracic spinal cord of Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(N) EM pictures showing degenerated axons (arrows) in the spinal cord of Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(O and P) GFAP staining in Epg5+/− and Epg5−/−mice. DCST, dorsal corticospinal tract."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(A) Levels of LC3 and p62 in brain and spinal cord extracts from Epg5+/− and Epg5−/− mice. SC, spinal cord."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(B) Polyubiquitinated proteins in detergent-soluble (S) and -insoluble (I) fractions from brain and spinal cord homogenates of Epg5+/− and Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(C) p62 aggregates and ubiquitin-positive aggregates are absent from Epg5+/− mice but dramatically accumulate and are colocalized (arrows) in Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(D-F) p62 aggregates are detected in neurons (arrows; D) and oligodendrocytes (arrows; E) but absent from astrocytes (F) in Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(G) Cytoplasmic TDP-43 aggregates accumulate in motor neurons in the anterior horn of spinal cord in Epg5−/− mice. Arrows show cytoplasmic TDP-43 aggregates. DSCT, dorsal corticospinal tract. (H) Percentages of cytoplasmic TDP-43 aggregate-positive motor neurons in the fifth layer of cortex and anterior horn of the spinal cord in Epg5+/− and Epg5−/− mice. Means ± SEM of three mice are shown. LC, lateral column. Bars, 10 µm."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(A and B) Electromyography of the gastrocnemius muscle from a 3-mo-old Epg5−/− mouse showing fibrillations (A) and positive sharp waves (B). These defects were absent in control mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(C) At 10 mo, Epg5−/−mice showed high-amplitude and long-duration action potentials when conducting MUAP tests. The blue lines show the start and end of an action potential. Dur, duration; Amp, amplitude."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(D-F) H&amp;amp;amp;E staining of gastrocnemius muscles showed features of muscle degeneration in Epg5−/−mice. The arrow in E indicates centrally nucleated fibers."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(G) The transcription levels of the atrophy-related genes were up-regulated in gastrocnemius muscles of Epg5−/−mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(J) Levels of LC3-II and p62 in muscle extracts from Epg5+/− and Epg5−/− mice."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(K) Percentage of p62 aggregate-positive myofibers in Epg5+/− and Epg5−/− mice. Means ± SEM of three mice are shown."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(L) Accumulation and colocalization of p62 and ubiquitin aggregates in the gastrocnemius muscles of Epg5−/− mice but not controls. Bars: (D-F and L) 10 µm; (H and I) 1 µm."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(A) Number of LC3 puncta in Epg5+/− (control) and Epg5−/− MEFs."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(B) Western blot showing levels of LC3-I and LC3-II in control and Epg5−/− MEFs upon indicated treatment (Starv., starvation; Rapa., rapamycin; Bafilo., bafilomycin A1)."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(D) Colocalization ratio of LC3 and LAMP-1 in control and Epg5−/− MEFs. 50 cells were examined per time point in A and D."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(E) Colocalization of LC3 puncta and lysosomes in control and Epg5−/− MEFs 4 h after starvation."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(F) Under nutrient repletion conditions, almost no autophagic elements are detected in Epg5+/− MEFs. RER, rough ER; M, mitochondrion; N, nucleus. The arrowhead indicates a vacuole with a late residual body-like appearance (aAV-III). (G) Under nutrient repletion conditions, Epg5−/− MEFs accumulate a large number of autophagic vacuoles. Red arrow, a likely autophagosome; red arrowheads, complex vacuoles of the aAV-I type; white arrowheads, aAV-II vacuoles"
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(H and I) High magnification of autophagic elements in unstarved Epg5−/− MEFs. (H) The red arrow indicates a complex autophagic vacuole containing multilayered membrane structures (white arrowheads) and a lipid-like region (L). (I) The red arrow indicates an autophagosome containing cytoplasm and ribosomes. The red arrowhead points to an autophagosome containing a mitochondrion and cytoplasmic material with membranous structures. The white arrow shows an aAV-III vacuole."
NCBI gene IDs:
"Epg5: 100502841"
Figure legend: "(J) Compared with control cells, Rab5-labeled early endosomes are significantly enlarged in Epg5 siRNA-treated cells."
NCBI gene IDs:
"Epg5: 57724"
Figure legend: "(K and L) EGFR degradation in control and Epg5 knockdown cells."
NCBI gene IDs:
"Epg5: 57724"
Figure legend: "(M and N) Distribution of Alexa Fluor 488-conjugated transferrin at different time points in control siRNA- and Epg5 siRNA-treated cells. Higher magnification views are shown in the insets. Error bars indicate SEMs."
NCBI gene IDs:
"Epg5: 57724"
Figure legend: "Strong nuclear p53 signal in immunostainings of HGSOC organoids is indicative of a gain-of-function TP53 mutation. Scale bars: 100 µm (Tissue) and 50 µm (Organoid)."
NCBI gene IDs:
"TP53: 7157"
Figure legend: "Heat map of differentially expressed genes between cancer and healthy tissue/organoids reveals upregulation of several HGSOC biomarkers and reduction of FT differentiation markers in the cancer samples. Differential expression determined by single-color microarray for 8 different patient samples was significant for all genes with p &lt; 0.05 except for OVGP1 and TOP2A in organoids."
NCBI gene IDs:
"OVGP1: 5016
TOP2A: 7153"
Figure legend: "Experimental approach for genetic manipulation of FT epithelial cells (FTECs). FTECs were sequentially transduced in 2D culture with replication-deficient viruses containing specific shRNAs and different selection markers. After sorting cells were seeded in Matrigel for organoid formation. Scale bars: 2 cm (tissue), 200 µm (2D culture) and 500 µm (3D culture). Confirmation of robust knockdowns of p53, PTEN and RB in FT organoids on RNA and protein level. Relative mRNA levels were normalized to the WT mRNA level and are given as the mean ±SEM from 3 different donors. A representative western blot from one donor is depicted. RNA as well as protein samples were taken at passage 1 or 2 of each organoid line. KD - Knockdown, Vec Ctrl - Vector Control, WT- Wild type"
NCBI gene IDs:
"PTEN: 5728
RB: 5925
p53: 7157"
Figure legend: "Cyclin E1 overexpression as a downstream effect of RB knockdown was confirmed by western blot analysis."
NCBI gene IDs:
"RB: 5925"
Figure legend: "Confirmation of robust knockdowns of p53, PTEN and RB in FT organoids on RNA and protein level. Relative mRNA levels were normalized to the WT mRNA level and are given as the mean ±SEM from 3 different donors. A representative western blot from one donor is depicted. RNA as well as protein samples were taken at passage 1 or 2 of each organoid line. KD - Knockdown, Vec Ctrl - Vector Control, WT- Wild type Cyclin E1 overexpression as a downstream effect of RB knockdown was confirmed by western blot analysis. Presence of ciliated cells (arrowheads), as revealed by immunostaining against detyrosinated Tubulin (dtyrTub) in triple KD organoids, proves capacity for terminal differentiation. Scale bar: 50µm."
NCBI gene IDs:
"PTEN: 5728
RB: 5925
p53: 7157"
Figure legend: "Triple KD (p53/PTEN/RB) organoids are characterized by a HGSOC gene expression signature as revealed by microarray analysis comparing WT and KD organoids grown in either OCM or FTM. Differential expression determined by dual-color microarray for 2 biological replicates was significant for all genes with p &lt; 0.00005."
NCBI gene IDs:
"PTEN: 5728
RB: 5925
p53: 7157"
Figure legend: "Differential expression of stemness- and differentiation-related genes of triple KD organoids grown in OCM vs. FTM. Fold-changes were derived from microarray data and indicate and an increase in stemness (CD133, SOX2, MYCN) and a drop in differentiation (OVGP1, PGR, FOXJ1) under OCM conditions. Differential expression determined by dual-color microarray for 2 biological replicates was significant for all genes with p &lt; 0.05."
NCBI gene IDs:
"FOXJ1: 2302
MYCN: 4613
OVGP1: 5016
PGR: 5241
CD133: 8842
SOX2: 6657"
Figure legend: "FOXJ1 expression determined by qPCR was significantly diminished in triple KD organoids grown in OCM compared to FTM, suggesting a decrease in differentiation capacity. Data represent the mean ±SEM from technical triplicates for two independent knockdown cultures."
NCBI gene IDs:
"FOXJ1: 2302"
Figure legend: "Comparative analysis of microarray data revealed consistent up-regulation of MYCN, the canonical Wnt inhibitors KREMEN2 and DKK3 as well as differentiation marker FOXJ1, AR and PGR in HGSOC cancer organoids as well as triple KD FT organoids. Differential expression was determined either by single-color microarray for the cancer samples (8 replicates) or dual-color microarray for the knockdowns (2 replicates) and is significant for all genes with p &lt; 0.05."
NCBI gene IDs:
"AR: 367
DKK3: 27122
FOXJ1: 2302
KREMEN2: 79412
MYCN: 4613
PGR: 5241"
Figure legend: "Transcriptional induction of MYCN is confirmed by qPCR for three patients between cancer organoids and normal FT organoids as well as triple KD organoids grown in OCM vs. FTM conditions. Depicted is the mean ±SEM of the normalized MYCN mRNA expression level of three biological replicates (n=3). ** p &lt; 0.01, *** p &lt; 0.001, two-sided Student`s t-test."
NCBI gene IDs:
"MYCN: 4613"
Figure legend: "FOXJ1 expression was upregulated in HGSOC organoids in FTM compared to OCM as shown by qPCR analysis of 3 different patient samples. Data represent the mean ±SEM of technical triplicates."
NCBI gene IDs:
"FOXJ1: 2302"
Figure legend: "A. qPCR analysis shows loss of Setd1b in forebrain regions while levels in the cerebellum are not affected (CA: Control, n = 6; cKO, n = 6. DG: Control, n = 6; cKO, n = 6. Cortex: Control, n = 7; cKO, n = 7. Cerebellum: Control, n = 4; cKO, n = 4). * p-value &lt; 0.05 (Student t-test)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "B. Immunoblot analysis shows loss of Setd1b protein in the hippocampus of Setd1b cKO mice (Control, n = 4; cKO, n = 4). ** p-value &lt; 0.01 (Student t-test)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "C. Immunohistochemical staining (upper level) for marker proteins of neuronal integrity and quantification (lower panel) show no difference in control and Setd1b cKO mice (NeuN: Control, n = 6; cKO, n = 6; Student t-test p-value = 0.57. MAP2: Control, n = 4; cKO, n = 4; Student t-test p-value = 0.72. Iba1: Control, n = 5; cKO, n = 5; Student t-test p-value = 0.8. Gfap: Control, n = 5; cKO, n = 5; Student t-test p-value = 0.09.). Scale bar: 100 µm."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "D. Average speed (left panel) and percent time spent in the center (right panel) during exposure to the open field test was similar in control and Setd1b cKO mice (Average speed: Control, n = 15; cKO, n = 15; Student t-test p-value = 0.075. Time spent in center: Control, n = 15; cKO, n = 15; Student t-test p-value = 0.96)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "E. Short term memory was not different between control and Setd1b cKO mice as indicated by similar percent of alternations in the Y-maze test (Control, n = 15; cKO, n = 15; Student t-test p-value = 0.3)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "G. Escape latency during water maze training indicated severe learning impairment in Setd1b cKO mice (Control: n = 15, cKO: n = 15. Repeated measures ANOVA, genotype effect: F (1,28) = 82.34, **** p-value &lt; 0.0001)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "H. Plots showing the specific search strategies during water maze training. Note the failure of Setd1b cKO mice to adopt hippocampus-dependent search strategies. I. The cognitive score calculated on the basis of the hippocampal search strategies reflects severe memory impairment in Setd1b cKO mice (Student t-test: *** p-value &lt; 0.001)."
NCBI gene IDs:
"Setd1b: 208043"
Figure legend: "F. Left panel shows representative images of RNAscope performed for Kmt2a, Kmt2b and Setd1b. Right panel shows a bar chart quantifying the dots/cell (n = 1500 cells from 2 mice) indicative of the corresponding expression level. Kmt2a expression was significantly higher when compared to Kmt2b or Setd1b (*** p-value &lt; 0,0001, Student t-test)."
NCBI gene IDs:
"Kmt2a: 214162
Kmt2b: 75410
Setd1b: 208043"
Figure legend: "Heatmap of IFNG, GZMB and PRF1 gene expression in COVID-19 patients. Average expression values were centered and scaled. Red indicates a higher expression and blue indicates a lower expression."
NCBI gene IDs:
"GZMB: 3002
IFNG: 3458
PRF1: 5551"
Figure legend: "LN229 were transfected with siNT, siNoxa 1, siNoxa 2 or BAK-siRNA for 72h. Thereafter, cells were treated with the combination treatment of 1 µM ABT263 and 20 µM LXR623 for another 24h. After conclusion of the treatment, cells were harvested, fixed, stained with propidium iodide and analyzed by flow cytometry for DNA - fragmentation."
NCBI gene IDs:
"BAK: 578
Noxa: 5366"
Figure legend: "U87 cells were transfected with non-targeting siRNA (n.t.-siRNA), ATF3-siRNA, ATF4 siRNA or the combination of both ATF4 and ATF3 siRNAs. After 48h, cells were treated with LXR623, whole cell protein lysates were harvested and analyzed by capillary electrophoresis for the expression of ATF3, ATF4, Noxa and Vinculin."
NCBI gene IDs:
"ATF3: 467
ATF4: 468"