EMBO/SourceData huggingface dataset:
Figure legend: "(C) m6A methylated RNA immunoprecipitation &amp; quantitative PCR (MeRIP-qPCR) analysis using a m6A antibody in NK92-siFTO (upper) or NK92+FTO (lower) cells compared to NK92-siCTRL or NK92+MOCK cells."
NCBI gene IDs:
"FTO: 79068"
Figure legend: "(D) SOCS3 3'-UTR reporter assay using the AANAT reporter system. Plasmid information for the AANAT reporter system with or without the SOCS3 3'-UTR sequence (upper). Levels of Aanat protein (left) and Aanat mRNA (right) expression in HEK-293T cells co-transfected with plasmid-expressing AANAT reporter containing SOCS3 3'-UTR and siCTRL or siFTO."
NCBI gene IDs:
"Aanat: 15
FTO: 79068
SOCS3: 9021"
Figure legend: "(B) Relative mRNA expression levels of JAK/STAT target genes, IFNγ (n = 10 biological replicates), GZMB (n = 6 biological replicates) and PRF1 (n = 8 biological replicates) were determined by qPCR in CD56+-siCTRL or CD56+-siFTO cells."
NCBI gene IDs:
"FTO: 79068
GZMB: 3002
IFNγ: 3458
PRF1: 5551"
Figure legend: "(C) Western blotting of CD56+-siCTRL or CD56+-siFTO cells incubated with 20 ng/ml IL-15 and 20 ng/ml IL-21 for the indicated times was performed. Cells were lysed and analyzed by immunoblotting with antibodies to the indicated phosphorylated (p-) and total proteins (n = 2~3). Data are representative of three times independent experiments with similar results. The protein levels were expressed as the relative band density of the corresponding protein (lower)."
NCBI gene IDs:
"FTO: 79068
IL-15: 3600
IL-21: 59067"
Figure legend: "(E) The images of the ventral bioluminescence (BLI) following the injection of NK92-TRC or NK92-shFTO cells."
NCBI gene IDs:
"FTO: 79068"
Figure legend: "(G) The survival curves after the injection of K562-Luc cells."
NCBI gene IDs:
"Luc: "
Figure legend: "(a-c) At 12-16 h post-transfection with GFP-LC3, NIH3T3 (a, b), MEF (b) or HCT116 (c) cells containing vector or the FLIP gene were treated with Hank's solution for 2-4 h (a) or with 2 μM rapamycin for 1-3 h (b, c). Subsequently, GFP-LC3 was detected using an inverted fluorescence microscope. Scale bar, 10 μm (a). The number of GFP-LC3-positive dots per cell was counted using a fluorescence microscope. Data are mean ± s.e.m.; n = 200-300 cells; three independent experiments; *P 0.01; **P 0.05) (b, c). At 60 h post-transfection with control siRNA or cFLIP siRNA, MEF cells were transfected with GFP-LC3 (c)."
NCBI gene IDs:
"FLIP: 
LC3: 
FLIP: 8837
LC3: 84557"
Figure legend: "(e, f) NIH3T3 vector and NIH3T3-KSHV-vFLIP cells (e) or TREX-BCBL-Vector and TREX-BCBL-vFLIP cells (f) were treated with Hank's solution or 2 μM rapamycin for 2-4 h, respectively, followed by IB with anti-LC3 and anti-actin antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(g) At 12-16 h post-transfection with GFP-LC3, TREX-BCBL-vector and TREX-BCBL-vFLIP cells were treated with doxycycline for 24 h, followed by incubation with 2 μM rapamycin for an additional 12 h. GFP-LC3 was detected using an inverted fluorescence microscope. Scale bar, 5μm. The number of GFP-LC3-positive dots per cell was counted using a fluorescence microscope. Data are mean ± s.e.m.; n = 200-300 cells; three independent experiments; *P 0.01)."
NCBI gene IDs:
"LC3: 
vFLIP: "
Figure legend: "(a) BCBL cells were used for immunoprecipitation (IP) with mouse IgG or anti-Atg3, followed by immunoblotting (IB) with anti-cFLIP antibody (left panel). NIH3T3-KSHV-vFLIP cells were used for IP with mouse IgG or anti-Flag antibody, followed by IB with anti-Atg3 antibody (right panel)."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(c) Schematic diagram of human Atg3 (top). N, amino terminus; FR, flexible region; C, carboxy terminus. At 48 h post-transfection with GST or GST-Atg3 along with Flag-vFLIP (left) or GST or GST-Atg3 along with GFP-LC3 (right), HEK293T cells were used for GST pulldown, followed by IB with the indicated antibodies."
NCBI gene IDs:
"Atg3: 
vFLIP: 
Atg3: Q9NT62///Q9CPX6
LC3: 84557"
Figure legend: "(d) Schematic diagram of KSHV vFLIP (top). The black boxes indicate the K-α2 and K-α4 peptides. At 48 h post-transfection with V5-Atg3 and either GST-vFLIP DED1 mutants (middle) or GST-vFLIP DED2 mutants (bottom), HEK293T cells were used for GST pulldown, followed by IB with anti-V5 antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(e) At 48 h post-transfection with GST, GST-Atg3, and either Flag-vFLIP or Flag-vFLIP mAtg3, HEK293T cells were used for GST pulldown, followed by IB with anti-Flag antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(f) At 48 h post-transfection with GST-Atg3 and GFP-LC3 along with increasing amount of Flag-vFLIP, HEK293T cells were used for GST pulldown, followed by IB with anti-GFP or anti-Flag antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(g) NIH3T3 vector and NIH3T3 vFLIP cells were treated with 2 μM rapamycin for 4 h and used for IP with anti-Atg3 antibody, followed by IB with anti-LC3 antibody. Finally, whole-cell lysates (WCLs) were used for IB with the indicated antibodies to show expression (a-g)."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(b) At 12-16 h post-transfection with GFP-LC3, doxycycline-treated TREX-BCBL vector, TREX-BCBL-vFLIP and TREX-BCBL-vFLIP mutant cells were incubated with or without 50 nM rapamycin for 6 days (top) or 2 μM rapamycin for 12 h (bottom). The percentage of cell death (trypan blue staining) and apoptosis level (annexin V staining) of rapamycin-treated cells was quantified as mean ± s.d. of three independent experiments. The number of GFP-LC3-positive dots per cell was counted using a fluorescence microscope. Data are mean ± s.e.m.; n = 200-300 cells; three independent experiments; *P&lt;0.01."
NCBI gene IDs:
"vFLIP: 
LC3: 84557"
Figure legend: "(c) BCBL1 cells were transfected with control siRNA or Beclin1 siRNA and treated with or without rapamycin for 5 days. The percentage of cell death was determined by trypan blue staining. Data are mean ± s.e.m.; n = 200-300; three independent experiments; *P 0.05."
NCBI gene IDs:
"Beclin1: 8678"
Figure legend: "(d) TREX-BCBL vector and TREX-BCBL-vFLIP cells were mock-treated or treated with 50 nM rapamycin for the indicated periods of time. The results were quantified as mean ± s.d. of the combined results from three independent experiments; *P 0.001."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(e) HEK293 cells carrying KSHV or KSHVΔvFLIP were treated with 500 nM rapamycin for 6 days. The results were quantified as mean ± s.d. of the combined results from three independent experiments; *P 0.01. Trypan blue staining and a Beckman Coulter Z2 Particle Count and size analyzer (BC Z2 CS analyser) were used to determine cell death and cell growth analysis (b-e)."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(b) At 12-16 h post-transfection with GFP-LC3, TREX-BCBL vector and TREX-BCBL-vFLIP cells were treated with doxycycline for 24 h, followed by incubation with 30 μM of the TAT, K-α2 or K-α4 peptide (top left) or the TAT, C-α2, or C-α4 peptide (bottom left) for an additional 12 h. Subsequently, autophagy was quantified as means ± s.d. of the combined results from three independent experiments (2 μM rapamycin treatment was included as a control). TREX-BCBL vector and TREX-BCBL-vFLIP cells were treated with doxycycline for 24 h, followed by incubation with 0, 30, or 50 μM of the TAT, K-α2, or K-α4 peptide (top right) or the TAT, C-α2, or C-α4 peptide (bottom right) for an additional 12 h. Trypan blue staining was then used to determine cell death (as a percentage). Data are mean ± s.e.m.; n = 200-300; three independent experiments; *P 0.01; **P 0.05."
NCBI gene IDs:
"LC3: 
vFLIP: "
Figure legend: "(c) At 12-16 h post-transfection with GFP-LC3, TREX-BCBL vector and TREX-BCBL-vFLIP cells were treated with doxycycline for 24 h, followed by incubation with 30 μM of the TAT, K-α2 or K-α4 peptide (top left) or the TAT, C-α2, or C-α4 peptide (bottom left) for an additional 12 h. Subsequently, autophagy was quantified as means ± s.d. of the combined results from three independent experiments (2 μM rapamycin treatment was included as a control). TREX-BCBL vector and TREX-BCBL-vFLIP cells were treated with doxycycline for 24 h, followed by incubation with 0, 30, or 50 μM of the TAT, K-α2, or K-α4 peptide (top right) or the TAT, C-α2, or C-α4 peptide (bottom right) for an additional 12 h and GFP-LC3 puncta were subsequently detected using an inverted fluorescence microscope. Scale bar, 5 μm."
NCBI gene IDs:
"vFLIP: 
LC3: 84557"
Figure legend: "(e) TREX-BCBL-Vector and TREX-BCBL-vFLIP cells were treated with the K-α2 and the K-α4 (30 μM each) peptide for 12 h, followed by immunoblotting (IB) with anti-LC3 and anti-actin antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(a) At 16 h post-transfection with GST, GST-K-α2 or GST-K-α4 along with Flag-vFLIP (top) or Flag-cFLIPL (bottom), HEK293T cells were used for GST pulldown, followed by immunoblotting (IB) with anti-Flag or anti-FLIP antibody. Whole-cell lysates (WCLs) were used for IB with anti-GST, anti-Flag or anti-FLIP antibody."
NCBI gene IDs:
"vFLIP: 
FLIP: 8837
FLIP: 8837///12633"
Figure legend: "(b) At 16 h post-transfection with V5-Atg3 and Flag-vFLIP with increasing amounts of GST-K-α2 or GST-K-α4, HEK293T cells were used for GST pulldown, followed by IB with anti-Flag or anti-V5 antibody or for immunoprecipitation (IP) with anti-V5 antibody, followed by IB with anti-Flag antibody. WCLs were used for IB with anti-GST, anti-Flag or anti-V5 antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(c) At 12-16 h post-transfection with GST-Atg3 along with Flag-vFLIP (first), Flag-cFLIPS (second), or HA-LC3 (third) with increasing amounts of the K-α2+K-α4 peptides (top three panels) or C-α2+C-α4 peptides (bottom panel), HEK293T cells were used for GST pulldown, followed by IB with anti-Flag or anti-HA antibodies. WCLs were used for IB with anti-GST and anti-Flag antibody."
NCBI gene IDs:
"LC3: 
vFLIP: 
cFLIP: 8837"
Figure legend: "(d) At 12-16 h post-transfection with Flag-vFLIP, HA-LC3 and V5-Atg3, HEK293T cells were treated with increasing amounts of the K-α2+K-α4 peptides and used for IP anti-V5 antibody, followed by IB with anti-Flag or anti-HA antibody. WCLs were used for IB with anti-V5, anti-HA and anti-Flag antibody."
NCBI gene IDs:
"vFLIP: "
Figure legend: "(A-B) Mitochondria isolated from (A) hTim8aKO HEK cells with and without hTim8aFLAG re-expression, or (B) hTim8bKO HEK cells with and without hTim8bFLAG re-expression were compared to mitochondria isolated from control HEK293 cells by SDS-PAGE and immunoblot. Protein expression of the hTim8 protein was induced with tetracycline (Tet) for the indicated time. Representative of n=2 biological replicates."
NCBI gene IDs:
"FLAG: 
Tim8a: 1678
Tim8b: 26521"
Figure legend: "Affinity enrichment mass spectrometry. Mitochondria isolated from (C) hTim8aKO HEK cells expressing hTim8aFLAG, (D) hTim8bKO HEK cells expressing hTim8bFLAG, (E) hTim8aMUT SH cells expressing hTim8aFLAG, (F) hTim8bKO SH cells expressing hTim8bFLAG were treated with DSP crosslinker prior to immunoprecipitation with anti-FLAG resin. The log2 fold change in mean LFQ intensity is plotted against unpaired Student's t-test p-value (n=3 biological replicates). Curve indicates significantly enriched proteins. FDR&lt;0.01; (C) s0=5.5, (D) s0=4.5, (E) s0=2, (F) s0=5, Functional annotations manually curated and coloured: TIMM family proteins (purple), Complex I subunits and assembly factors (orange), Complex IV subunits and assembly factors (green), MIA import substrates (blue), significantly enriched (black)."
NCBI gene IDs:
"FLAG: 
Tim8a: 1678
Tim8b: 26521"
Figure legend: "Changes in protein abundance of hTim8a and hTim8b interactors in mitochondria isolated from (B) hTim8aKO HEK and hTim8bKO HEK, or (C) hTim8aMUT SH and hTim8bKO SH cells; determined by quantitative mass spectrometry as published in Quadrants are labelled with 8a/8b ↑ or ↓ to indicate the increase or decrease of protein abundances in each knock-out cell line. The log2 fold change in mean LFQ intensity between knock-out and control cells is plotted on each axis. Lines indicate fold change in abundance of ±1.5. n=3 biological replicates for each cell line. Functional annotations manually curated and coloured: TIMM family proteins (purple), Complex I subunits and assembly factors (orange), Complex IV subunits and assembly factors (green), MIA import substrates (blue), significantly altered (black)."
NCBI gene IDs:
"Tim8a: 1678
Tim8b: 26521"
Figure legend: "(B-C) Mitochondria isolated from (B) COX17MUT HEK and (C) COX17KO SH cells were analysed by quantitative mitochondrial proteomics relative to control cells. The log2 fold change in mean LFQ intensity is plotted against unpaired Student's t-test p-value (n=3 biological replicates). Curve indicates significantly altered proteins; FDR&lt;0.05; s0=0.5. Functional annotations manually curated and coloured: Complex I subunits and assembly factors (orange), Complex II subunits and assembly factors (yellow), Complex III subunits and assembly factors (red) Complex IV subunits and assembly factors (green), Complex V subunits and assembly factors (pink), TIMM family proteins (purple), significantly altered (black)."
NCBI gene IDs:
"COX17: 10063"
Figure legend: "(D) Mitochondria isolated from control cells, COX17MUT HEK and COX17KO SH cells were analysed by BN-PAGE and immunoblotting with the indicated antibodies. COX4I1-containing complexes indicated as SC: Respiratory supercomplex, CIII2+CIV: Complex III2-Complex IV complex, CIV2: Complex IV dimer, CIV: mature Complex IV, and S2*: COX4I1-containing S2 subcomplex. Representative of n=2 biological replicates."
NCBI gene IDs:
"COX17: 10063"
Figure legend: "(F) Intact cells or mitochondria isolated from hTim8bKO HEK and control HEK293 cells were subject to ICP-MS analysis to measure copper (Cu) and iron (Fe) content. Abundance is shown as mean ±S.D for metal content per gram of protein. Significance determined by unpaired Student's t-test (n=3 biological replicates). **, p&lt;0.01; 'ns' indicates not significant, p&gt;0.05."
NCBI gene IDs:
"Tim8b: 26521"
Figure legend: "(A) hTim8aKO HEK, hTim8bKO HEK and control HEK293 cells were pulsed with [35S]-Met/Cys for 2 h in the presence of anisomycin and chased for the indicated times. Isolated mitochondria were analysed by SDS-PAGE, auto-radiography and immunoblot. n=1 biological replicates."
NCBI gene IDs:
"Tim8a: 1678
Tim8b: 26521"
Figure legend: "[35S]-COX6C and [35S]-COX7C were incubated with mitochondria isolated from (B) hTim8aMUT SH control SH-SY5Y Import proceeded in the presence or absence of mitochondrial membrane potential (ΔΨ) for the indicated times. Samples were analysed by BN-PAGE and autoradiography. CBB = Coomassie brilliant blue staining. Representative of n=2 biological replicates."
NCBI gene IDs:
"Tim8a: 1678"
Figure legend: "[35S]-COX6C and [35S]-COX7C were incubated with mitochondria isolated from (C) hTim8bKO HEK and control HEK23 cells. Import proceeded in the presence or absence of mitochondrial membrane potential (ΔΨ) for the indicated times. Samples were analysed by BN-PAGE and autoradiography. CBB = Coomassie brilliant blue staining. Representative of n=2 biological replicates."
NCBI gene IDs:
"Tim8b: 26521"
Figure legend: "(F) Mitochondria isolated from control, hTim8aMUT SH and hTim8bKO HEK cells were analysed by BN-PAGE and immunoblotting. COX4I1-containing complexes indicated as SC: Respiratory supercomplex, CIII2+CIV: Complex III2-Complex IV complex, CIV2: Complex IV dimer, CIV: mature Complex IV, and S2*: COX4I1-containing S2 subcomplex. Levels of COX4I1 present in subcomplex (*) was quantified as mean ±S.D percentage of COX4I1 present in mature Complex IV (CIV) for each cell line. Significance determined by unpaired Student's t-test (hTim8bKO HEK n=6 biological replicates; hTim8aMUT SH n=4 biological replicates). **, p&lt;0.01."
NCBI gene IDs:
"Tim8a: 1678
Tim8b: 26521"
Figure legend: "(B-C) Profile plots of iBAQ intensity for Complex IV subunits across the complexome profile of mitochondria isolated from (B) hTim8aMUT SH and control SH-SY5Y cells; and (C) hTim8bKO HEK and control HEK293 cells. Subunits are plotted by Complex IV module and iBAQ intensity relative to the maximum intensity of plotted subunits. Solid line indicates subunits from control cells, dashed line from knock-out cells. Complex IV assemblies are indicated as SC: Respiratory supercomplex, CIII2+CIV: Complex III2-Complex IV complex, CIV: mature Complex IV, and S2*: COX4I1-containing S2 subcomplex. Representative of n=2 biological replicates. Data presented as iBAQ intensity, normalised as in (A) and expressed as a ratio of the maximum iBAQ intensity."
NCBI gene IDs:
"Tim8a: 1678
Tim8b: 26521"
Figure legend: "(A) Mitochondria isolated from hTim8aMUT SH cells with and without hTim8bFLAG expression compared to mitochondria from control SH-SY5Y cells were analysed by SDS-PAGE and immunoblotting. Amount of protein after 12 h induction (lanes 4 and 10) was quantified as mean ±S.D percentage of control SH-SY5Y mitochondria. Significance determined by unpaired Student's t-test (n=3 biological replicates). *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ****,p&lt;0.0001; 'ns' indicates not significant, p&gt;0.05."
NCBI gene IDs:
"FLAG: 
Tim8a: 1678
Tim8b: 26521"
Figure legend: "(C) Mitochondria isolated from hTim8aMUT SH cells with and without hTim8bFLAG expression compared to mitochondria isolated from control SH-SY5Y cells analysed by BN-PAGE and immunoblotting. COX4I1-containing complexes are indicated as SC: Respiratory supercomplex, CIII2+CIV: Complex III2-Complex IV complex, CIV2: Complex IV dimer, CIV: mature Complex IV, and S2*: COX4I1-containing S2 subcomplex. Amount of COX4I1 present in mature monomeric Complex IV (CIV) was quantified as mean ±S.D percentage of control SH-SY5Y mitochondria. Significance determined by unpaired Student's t-test (n=3 biological replicates). **, p&lt;0.001; ***, p&lt;0.001; 'ns' indicates not significant, p&gt;0.05."
NCBI gene IDs:
"FLAG: 
Tim8a: 1678
Tim8b: 26521"
Figure legend: "(D) Mitochondria isolated from hTim8bKO HEK cells with and without hTim8aFLAG expression compared to mitochondria isolated from control HEK293 cells by BN-PAGE. hTim8aFLAG expression induced by tetracycline for 8 h. Native protein complexes detected by immunoblot. COX4I1-containing complexes by immunoblot indicated as SC: Respiratory supercomplex, CIII2+CIV: Complex III2-Complex IV complex, CIV2: Complex IV dimer, CIV: mature Complex IV, and S2*: COX4I1-containing S2 subcomplex. Amount of COX4I1 present in mature monomeric Complex IV (CIV) was quantified as mean ±S.D percentage of control HEK293 mitochondria. Significance determined by unpaired Student's t-test (n=3 biological replicates). **, p&lt;0.01; 'ns' indicates not significant, p&gt;0.05."
NCBI gene IDs:
"FLAG: 
Tim8a: 1678
Tim8b: 26521"
Figure legend: "A Immunofluorescence staining   of KANK1  (red) and  α-tubulin (blue) in Strep-GFP-CEP170B  knock-in HeLa  cells. Lower panels show enlargements of the peripheral (1) and central (2) cortical  regions boxed in the upper panels. Data information: Scale bars, 5 μm."
NCBI gene IDs:
"GFP: 
CEP170B: 283638"
Figure legend: "C Immunofluorescence staining  of KANK1  in Strep-GFP-CEP170B  knock-in HeLa  cells treated with nocodazole. Lower panels show enlargements of boxed areas in the upper panels. D Immunofluorescence staining  of CEP170B  and α-tubulin in MCF7  cells. Lower panels show enlargements of boxed areas in the upper panels. Data information: Scale bars, 5 μm."
NCBI gene IDs:
"GFP: 
CEP170B: 283638"
Figure legend: "G Immunofluorescence staining  of KANK1, KANK2, or  liprin-α1  in control Strep-GFP-CEP170B  knock-in HeLa  cell line or indicated knockout (KO) cell lines that were generated based on the parental Strep-GFP-CEP170B  knock-in HeLa  cell line. Data information: Scale bars, 5 μ"
NCBI gene IDs:
"GFP: 
CEP170B: 283638"
Figure legend: "B Streptavidin pull-down assay with extracts of HEK293T  cells expressing Bio-GFP-tagged  liprin-α1 full length (FL) or its indicated fragments, analyzed by Western blotting with indicated antibodies. The Bio-GFP-tagged bait proteins were detected by IRDye® 680RD Streptavidin."
NCBI gene IDs:
"GFP: 
liprin-α1: 8500"
Figure legend: "D Streptavidin pull down  assay with extracts of HEK293T  cells expressing Bio-GFP-tagged wild-type (WT) liprin-α1 fragment N1 or its NR/AA mutant, analyzed by  Western blotting with indicated antibodies. The Bio-GFP-tagged bait proteins were detected by IRDye® 680RD Streptavidin. E Streptavidin pull down  assay with extracts of HEK293T  cells expressing Bio-GFP-tagged WT liprin-α1 N1 fragment or its NR/AA mutant (bait) together with GFP-CEP170B   (prey), analyzed by Western blotting with GFP  antibody."
NCBI gene IDs:
"GFP: 
liprin-α1: 8500"
Figure legend: "F Immunofluorescence staining  of  liprin-β1 in liprin-α1  KO HeLa  cells transiently transfected with mCherry-tagged liprin-α1  full length or its NR/AA mutant. G, H Averaged intensity distributions for WT or NR/AA versions of mCherry-liprin-α1 (G) and liprin-β1 (H) for the experiments shown in (F). Distance from the cell edge is shown on the horizontal axis. n = 25-29 cells from three technical replicates. Data represent mean ± SEM. Data information: Scale bars, 5 μm."
NCBI gene IDs:
"mCherry: 
liprin-α1: 8500"
Figure legend: "J, K Immunofluorescence staining  of α-tubulin  (blue) in  liprin-α1 KO/Strep-GFP-CEP170B  knock-in double-engineered HeLa  cell line transiently transfected with mCherry-tagged liprin-α1 FL (J), fragment N1 (K), or their NR/AA mutant counterparts (J, K). Data information: Scale bars, 5 μm."
NCBI gene IDs:
"GFP: 
mCherry: 
CEP170B: 283638
liprin-α1: 8500"
Figure legend: "A TIRF microscopy time-lapse images on the left show the accumulation of CEP170B  along the distal segments of MT  lattices in the cortical  region of Strep-GFP-CEP170B  knock-in HeLa  cell line transiently transfected with EB3-TagRFP. Representative kymographs   on the right show an MT  plus end undergoing very short alternating growth and shrinking episodes (corresponding to the one marked as \"1\" in the images; upper right panel) and an MT  plus end undergoing longer depolymerization followed by rescues (corresponding to the one marked as \"2\" in the images; bottom right panel ). Scale bars: horizontal, 2 μm; vertical, 30 s. Data information: Scale bars, 5 μm."
NCBI gene IDs:
"GFP: 
RFP: 
CEP170B: 283638
EB3: 22924"
Figure legend: "D Immunofluorescence staining  of CAMSAP2  (red) and  α-tubulin (blue) in HeLa  cells transiently transfected with GFP  control or GFP-CEP170B. Lower panels show enlargements of boxed areas. E Quantification of the length of CAMSAP2  stretches for the experiments shown in (D). n = 12 cells from three technical replicates. Data information: Unless otherwise stated, Data represent mean ± SD. *, P&lt;0.05; **, P &lt; 0.01; ***, P &lt; 0.001; two-tailed t test. Scale bars, 5 μm."
NCBI gene IDs:
"GFP: 
CEP170B: 283638"
Figure legend: "C Immunofluorescence staining  of CAMSAP2  (green) and α-tubulin (red) in control or CEP170B  KO MCF7  monolayer. Left panels: basal plane; middle panels: apical plane; right panels: side view. D Immunofluorescence staining  of α-tubulin (red) in control CAMSAP3-GFP  knock-in  Caco-2 monolayer or CEP170B  KO/CAMSAP3-GFP  knock-in double engineered Caco-2 monolayer. Left panels: basal plane; middle panels: apical plane; right panels: side view. Data information: Scale bars: 5 μm"
NCBI gene IDs:
"GFP: 
CAMSAP3: 69697
CEP170B: 283638"
Figure legend: "G Caco-2  cells were cultured in Matrigel  for 5 days, and subsequently treated with  Cholera toxin  for 12 hours. Phase-contrast images of thus  formed control or CEP170B  KO Caco-2 cysts  were shown. H, I Representative images and quantification of four categories of lumen  formation for experiments shown in (G). n = 100 cysts  from three technical replicates. J Quantification of the diameter of control and CEP170B  KO Caco-2 cysts. n = 30 cysts  from three technical replicates. Data information: Data represents the mean ± SD. ***, P &lt; 0.001; two-tailed t test. Scale bars: 25 μm"
NCBI gene IDs:
"CEP170B: 283638"