EMBO/SourceData huggingface dataset:
Figure legend: "Time-lapse of HeLa cells expressing GFP-alpha tubulin and RFP-lifeact treated with control siRNA or siRNA against WASH1 for 48 hours arrested at prometaphase and forced to exit mitosis with Ro3306 addition (t=0). Scale bar = 5µm."
NCBI gene IDs:
"WASH1: 100287171"
Figure legend: "Quantification of actin around the centrosome in (A), showing the failure to accumulate actin around the centrosome in siWASH. N = 4 experiments. Error bars represent standard deviation."
NCBI gene IDs:
"WASH: 100287171"
Figure legend: "Western blots of lysates from cells treated with siRNA control and siRNA against WASH1 probed with WASH1 antibody, showing the reduction in WASH levels following treatment of cells with siRNA against WASH. Scale bar = 5µm and 2µm in zoom."
NCBI gene IDs:
"WASH: 100287171
WASH1: 100287171"
Figure legend: "Z-projection of HeLa cells expressing GFP-centrin1 treated with control siRNA or siRNA against WASH, arrested at prometaphase or forced to exit mitosis and immunostained with phalloidin. Scale bar-5µm Quantification of actin around the centrosome in (F), showing the failure to accumulated actin around the centrosome during forced exit in cells treated with siRNA against WASH. siControl-STLC= 1 ± 0.0189, n=165, siWASH-STLC=0.9138 ± 0.0298, n=118, siControl-STLC+RO-3306=1.911 ± 0.06221, n=147, siWASH-STLC+RO-3306=1.103 ± 0.0301, n=140. Error bars represent standard deviation. P&lt;0.0001, one-way ANOVA."
NCBI gene IDs:
"WASH: 100287171"
Figure legend: "G Representative lung sections from WT or IFITM3 KO animals stained for SARS-CoV-2 N protein. Scale bars represent 2 mm for whole lung images. Colored boxes overlaying the whole lung images correspond to the displayed magnified regions and scale bars in these images represent 0.2 mm. Red triangles, infected airways; solid black arrow, non-infected blood vessels; open black arrow, infected blood vessels."
NCBI gene IDs:
"IFITM3: 66141"
Figure legend: "K18-hACE2 (hACE2) and K18-hACE2/IFITM3 KO mice were infected with 104 TCID SARS-CoV-2 WA1. A Weight loss (data points represent mean and error bars represent SEM, data from two independent experiments [hACE2 n=7, hACE2/IFITM3 KO n=8], skull/crossbones indicates that KO mice did not survive beyond this timepoint, *p &lt; 0.05, ** p &lt; 0.01, ANOVA with Bonferoni's multiple comparison's test)."
NCBI gene IDs:
"ACE2: 59272
IFITM3: 66141"
Figure legend: "K18-hACE2 (hACE2) and K18-hACE2/IFITM3 KO mice were infected with 104 TCID SARS-CoV-2 WA1. C Viral titers in extrapulmonary organs on the indicated days post infection were measured (bars/error bars represent mean ± SD, data points represent distinct biological samples, day 3 data for heart, spleen, and brain are from two independent experiments, day 3 data for liver and kidney and day 5 data are from single experiments, data points at the x axis represent samples below the limit of detection, **p &lt; 0.01, Mann-Whitney test)."
NCBI gene IDs:
"ACE2: 59272
IFITM3: 66141"
Figure legend: "K18-hACE2 (hACE2) and K18-hACE2/IFITM3 KO mice were infected with 104 TCID SARS-CoV-2 WA1. D Representative lung sections from WT mice infected with SARS-CoV-2 MA10 (105 TCID) or K18-hACE2 or K18-hACE2/IFITM3 KO mice infected with SARS-CoV-2 WA1 (104 TCID) stained for SARS-CoV-2 N protein at day 5 post infection. Scale bars represent 2mm."
NCBI gene IDs:
"ACE2: 59272
IFITM3: 66141"
Figure legend: "WT and IFITM3 KO mice were intranasally infected with 105 TCID50 SARS-CoV-2 MA10 or mock infected with PBS (Non-Infected, NI). A Representative lung images from mice at the indicated times post infection were stained with hematoxylin and eosin. Scale bars represent 2mm for whole lung images and 500um for zoomed images. B Staining quantifications from multiple mice are shown (bars/error bars represent mean ± SD, data points represent distinct biological samples, *p &lt; 0.05, ANOVA with Bonferoni's multiple comparison's test)."
NCBI gene IDs:
"IFITM3: 66141"
Figure legend: "WT and IFITM3 KO mice were intranasally infected with 105 TCID50 SARS-CoV-2 MA10 or mock infected with PBS (Non-Infected, NI). A Representative lung images from mice at the indicated times post infection were stained with anti-CD45. Scale bars represent 2mm for whole lung images and 500um for zoomed images. B Staining quantifications from multiple mice are shown (bars/error bars represent mean ± SD, data points represent distinct biological samples, *p &lt; 0.05, ANOVA with Bonferoni's multiple comparison's test)."
NCBI gene IDs:
"IFITM3: 66141"
Figure legend: "WT and IFITM3 KO mice were intranasally infected with 105 TCID50 SARS-CoV-2 MA10. RNA sequencing analysis was performed on RNA extracted from infected WT and IFITM3 KO lungs on day 2 post infection. D Relative gene expression heat maps for genes from the top two most significant GO Biological Processes as shown in C."
NCBI gene IDs:
"IFITM3: 66141"
Figure legend: "(D) Top: Schematic of GFP expression reporter system, with the CBASS promoter, capH, and capP genes from E. coli MS115-1 and the CBASS core genes replaced with GFP. Bottom: Western blot showing GFP expression in cells with the wild-type GFP reporter or constructs lacking either capP or capH genes. RNAP: RNA polymerase loading control."
NCBI gene IDs:
"capH: 
capP: 
GFP: "
Figure legend: "(E) Western blots of the CBASS expression reporter system with FLAG-NucC showing FLAG-NucC expression after infection with phage λ cI- (multiplicity of infection: 10). MPI: minutes post infection. α-RNAP: anti-RNA polymerase loading control. Low RNAP expression at later time points is due to cell death."
NCBI gene IDs:
"cI: 3827059"
Figure legend: "(E) GFP expression reporter assay showing loss of suppression upon mutation of CapH. RNAP: RNA polymerase loading control."
NCBI gene IDs:
"CapH: "
Figure legend: "(A) Anti-GFP western blot showing coexpression in E. coli of an MBP-CapH-GFP fusion construct with wild-type or catalytic-dead (E98Q) CapP. Full-length MBP-CapH-GFP is indicated with a yellow arrowhead, and the C-terminal product of CapP cleavage is indicated with a white arrowhead. RNAP: RNA polymerase loading control."
NCBI gene IDs:
"CapP: "
Figure legend: "(F) GFP reporter assay showing effect of a CapP E98Q catalytic-dead mutant, CapH R83A mutant, or removal of CapH residues 83-107 (capH 1-82) on GFP expression. RNAP: RNA polymerase loading control."
NCBI gene IDs:
"CapH: 
CapP: "
Figure legend: "(G) CBASS expression reporter system with FLAG-NucC, showing effect of a CapH R83A mutant, or removal of CapH residues 83-107 (capH 1-82) on FLAG-NucC expression. α-RNAP: anti-RNA polymerase loading control."
NCBI gene IDs:
"capH: 
CapH: "
Figure legend: " A. In vivo genetic complementation of the E. coli MC4100 wild-type (WT) and secB or/and tig knock-out derivatives by either an empty vector or one carrying the secB or tig (encoding TF) genes or derivatives (see &quot;Plasmid&quot; table in Appendix), as indicated. Serial dilutions of a culture (OD600= 0.5) were spotted (12μl) on LB-Ampicillin plates containing or not anhydrotetracycline (AHT; 5 ng/ml) and grown at 16°C or 30°C (as indicated). n= 3-7 biological replicates. "
NCBI gene IDs:
"secB: 948123
tig: 945081"
Figure legend: "A,B) Relative gene expression analysis of the pro- and anti-apoptotic markers Bax, Bak, Bcl-2, and Bcl-xl in Cal-78 and SW-1353 cells treated with the respective IC50 values of bortezomib for 24 h (n = 10)."
NCBI gene IDs:
"Bak: 578
Bax: 581
Bcl-2: 596
Bcl-xl: 598"
Figure legend: "A,B) Relative gene expression analysis of IGF1R, Fas, and the death receptors TRAILR-1 and TRAILR-2 after bortezomib treatment with the respective IC50 concentrations for 48 h. Untreated control cells served as reference value (ratio = 1)."
NCBI gene IDs:
"Fas: 355
IGF1R: 3480
TRAILR-1: 8797
TRAILR-2: 8795"
Figure legend: "A) Relative gene expression and B) western blot analysis of whole cell lysates for the expression of the autophagy markers Atg 5/12 and Beclin in Cal-78 and SW-1353 cells treated with the respective IC50 values of bortezomib for 24 h."
NCBI gene IDs:
"Atg 5: 9474
Beclin: 8678"
Figure legend: "Cell viability analysis of HeLa  cells stably expressing the sh-NC and sh-GAPDH  were treated with the indicated concentration of ADR  for 48 h. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Left, representative immunofluorescence  images of γH2AX  (C, green) in HeLa  cells transfected with scramble siRNA or si-GAPDH  after 4 Gy IR  exposure and recovered for 4 h. DNA was stained  with DAPI  (blue). Right quantification of γH2AX  foci/cell (n=50). Mann-Whitney U-test. Scale bars, 10 μm. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Left, representative immunofluorescence  images of 53BP1  (D, red) in HeLa  cells transfected with scramble siRNA or si-GAPDH  after 4 Gy IR  exposure and recovered for 4 h. DNA was stained  with DAPI  (blue). Right, quantification of 53BP1  foci/cell (n=50). Mann-Whitney U-test. Scale bars, 10 μm. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot (left) and quantification (right) of γH2AX  in HeLa  cells pre-transfected with scrambled siRNA or si-GAPDH, recovered at indicated points after 4 Gy IR  exposure. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot  (left) and quantification (right) of HR proteins in HeLa  cells transfected with scrambled siRNA and si-GAPDH. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Quantification of RAD51  (left) or γH2AX  (right) foci/cell in HeLa  cells pre-transfected with scrambled siRNA or si-GAPDH, recovered at the indicated  time points after 4 Gy IR  exposure (n=50). Mann-Whitney U-test. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot  (left) and quantification (right) of RAD51  in HeLa  cells with or without GAPDH  knockdown treated with 50 μg/mL CHX  at the indicated time points. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot  (left) and quantification (right) of RAD51  in GAPDH  knockdown HeLa  cells and control cells treated with or without MG132  (10 μM) for 10 h. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot  of cell lysates or anti-RAD51 immunoprecipitates  from HeLa  cells with scramble siRNA or si-GAPDH  transfection treated with MG132  (10 μM) for 2 h."
NCBI gene IDs:
"GAPDH: 2597"
Figure legend: "Immunoblot  (left) and quantification (right) of Flag-RAD51  in cell lysates from HEK293T  cells transfected with indicated Flag-RAD51  mutations. Endogenous RAD51  and Flag-RAD51  are denoted respectively. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"Flag: 
RAD51: 5888"
Figure legend: "Immunoblot  of cell lysates or anti-Flag immunoprecipitates  from HEK293T  cells transfected with indicated Flag-RAD51  mutations and then exposed to 10 μM MG132. Immunoblot  of cell lysates or anti-Flag immunoprecipitates  from HEK293T  cells transfected with HA-Ubiquitin   and indicated Flag-RAD51  mutations and then exposed to 10 μM MG132."
NCBI gene IDs:
"Flag: 
HA: 
RAD51: 5888"
Figure legend: "Immunoblot  (upper) and quantification (lower) of RAD51  in HEK293T  cells transfected with empty vector, Flag-HDAC1, Flag-HDAC1-S3 (∆306-353), and Flag-HDAC1-H141A. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"Flag: 
HDAC1: 3065"
Figure legend: "HeLa  cells were transfected with empty vector, Flag-HDAC1, and Flag-HDAC1-H141A, then subjected to immunoprecipitation  and immunoblots as indicated."
NCBI gene IDs:
"Flag: 
HDAC1: 3065"
Figure legend: "Left, GAPDH  knockdown HeLa  cells transfected with Flag-HDAC1  were treated with 4 Gy IR, fixed after 4 h, and immunostained  with anti-RAD51  (red). DNA was stained  with DAPI  (blue). The number of RAD51  foci per cell is shown (right, n=50). Mann-Whitney U-test. Scale bars, 5 μm. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"Flag: 
GAPDH: 2597
HDAC1: 3065"
Figure legend: "Immunoblot  (upper) and quantification (lower) of γH2AX  in GAPDH  knockdown HeLa  cells transfected with or without Flag-HDAC1  at the different time points after 4 Gy IR  exposure. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"Flag: 
GAPDH: 2597
HDAC1: 3065"
Figure legend: "Left, GAPDH  knockdown HeLa  cells transfected with Flag-HDAC1  were treated with 4 Gy IR, fixed after 4 h, and immunostained  with anti- γH2AX  (green). DNA was stained  with DAPI  (blue). The number of γH2AX  foci per cell is shown (right, n=50). Mann-Whitney U-test. Scale bars, 5 μm. Data information: Data represented as mean ± s.d. of at least three independent experiments. P values are from Student's t-tests. *P&lt;0.05; **P&lt;0.01; ***P&lt;0.001; ns: not significant"
NCBI gene IDs:
"Flag: 
GAPDH: 2597
HDAC1: 3065"
Figure legend: "B. Dot blot of tau levels in media from HEK293T cells overexpressing tau and FLAG-DnaJC5 compared to empty vector and tau. Intracellular protein levels are shown by Western blot, DnaJC5 was detected by FLAG antibody. Quantification of extracellular tau levels shown below; data are mean ± SEM, n=8, ***p&lt;0.001."
NCBI gene IDs:
"DnaJC5: 79130
tau: 4137"
Figure legend: "C. Light/heavy ratio of tau peptides in label-free mass spectroscopy of media from HEK293T cells overexpressing tau or tau and DnaJC5 compared to 100 ng 15N labelled recombinant tau. Light peptides represent endogenous tau peptides. Data are mean ± SEM, n=3 biological repeats, ***p&lt;0.001."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "A. Western blot of endogenous tau levels from M17 neuroblastoma cells overexpressing FLAG-DnaJC5 or empty vector. Media was concentrated prior to analysis. Intracellular DnaJC5 was detected with FLAG antibody. Quantification of extracellular tau levels are mean ± SEM, n=3, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "B. Western blottau levels from concentrated media from ex-vivo organotypic slice cultures of wildtype mice overexpressing GFP-AAV9 or FLAG-DnaJC5-AAV9. DnaJC5 is detected by FLAG antibody. Quantification of extracellular tau levels are mean ± SEM, n=3, **p&lt;0.01 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "C. Western blottau levels from concentrated media from ex-vivo organotypic slice cultures of Cspa+/+ or Cspa-/- mice. DnaJC5 was detected by DnaJC5 antibody (Synaptic Systems). Quantification of extracellular tau levels are mean ± SEM, n=3, **p&lt;0.01 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"Cspa: 13002"
Figure legend: "A. FLAG-DnaJC5 overexpression facilitates extracellular release of wildtype (WT), P301L and R406W tau by dot blot in HEK293T cells. Intracellular protein levels are shown by Western blot; DnaJC5 was detected by FLAG antibody. Quantification of extracellular tau levels shown below; data are mean ± SEM, n=3, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130
tau: 4137"
Figure legend: "B. Overexpression of FLAG-DnaJC5 induces extracellular release of wildtype (WT) and A53T α-synuclein in HEK293T cells. Extracellular protein levels shown by dot blot (quantification below, mean ±SEM, n=3 ***p&lt;0.001, ** p&lt;0.01 by one-way analysis of variance with Tukey's multiple comparison post-hoc analysis) and intracellular proteins levels were analyzed by Western blot, DnaJC5 was detected by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130
α-synuclein: 6622"
Figure legend: "C. DnaJC5 overexpression induces extracellular release of GFP-tagged wildtype (WT), A315T and Q343R TDP-43 in HEK293T cells. Intracellular DnaJC5 was detected by FLAG antibody. Extracellular protein levels are analyzed by dot blot (quantification below, mean ±SEM, n=4) ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130
TDP-43: 23435"
Figure legend: "A. Dot blot of tau levels in media of cells overexpressing the phospho-deficient FLAG-DnaJC5 S10A compared to FLAG-DnaJC5 WT. Intracellular levels shown by Western blot, DnaJC5 was detected by FLAG antibody. Quantification of tau levels are mean ± SEM, n=6, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "B. Knockdown of SNAP23 by siRNA inhibits DnaJC5-mediated tau release in HEK293T cells as shown by dot blot. Quantification of extracellular levels are mean ± SEM, n=3, **p&lt;0.01 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis. DnaJC5 was detected by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130
SNAP23: 8773"
Figure legend: "C. Knockdown of SNAP23 by siRNA inhibits DnaJC5-mediated α-synuclein release in M17 cells as shown by dot blot. Quantification of extracellular levels are mean ± SEM, n=3, **p&lt;0.01 by one-way ANOVA with a Tukey's post-hoc analysis. DnaJC5 is detected by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130
SNAP23: 8773"
Figure legend: "D. Knockdown of SNAP23 by siRNA inhibits DnaJC5-mediated TDP-43 release in M17 cells as shown by dot blot. Quantification of extracellular levels are mean ± SEM, n=3, **p&lt;0.01 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis. DnaJC5 was detected by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130
SNAP23: 8773"
Figure legend: "A. Confocal microscopy of tau (red pseudocolor) and Hsp70 (green pseudocolor) colocalization (merged image) levels in wild type primary neurons overexpressing GFP-AAV9 or FLAG-DnaJC5-AAV9. Boxed area are 5X digital zoom on a 60X lens shown below. Scale bars are 20 µm and 5 µm as indicated. Only neurons overexpressing virus were imaged.B. Quantification of tau-Hsp70 co-localization (mean Pearson's coefficient ± SEM, n=18, *p &lt;0.05, one-way analysis of variance with Tukey's multiple comparison post-hoc analysis)."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "D. Western blot of HEK293T intracellular lysates show the DnaJC5/Hsc70/tau complex is disrupted by the addition of increasing amounts of YM-01 (0, 10, 30, 100 µM) in a dose-dependent fashion. DnaJC5 was detected by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "E. The DnaJC5/Hsc70 complex is disrupted when Hsc70 activity is inhibited by a dominant-negative point mutation, E175S. Western blot of immunoprecipitated DnaJC5 from HEK293T cells overexpressing WT or DN E175S Hsc70. DnaJC5 is immunopreicipitated by DnaJC5 antibody (Synaptic Systems). DnaJC5 was detected on Western blot by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 79130
Hsc70: 3312"
Figure legend: "G. Dot blot of tau levels in media of primary neurons from transgenic overexpressing tau P301L mice treated with YM-01 (10µM). FLAG-DnaJC5 was transduced into neurons using AAV9. Quantification of extracellular tau levels are mean ± SEM, n=6, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis. DnaJC5 was detected on Western blot by FLAG antibody."
NCBI gene IDs:
"DnaJC5: 13002"
Figure legend: "A. Chemical inhibition of Hsc70 activity in HEK293T cells overexpressing FLAG-DnaJC5 reduces extracellular tau levels as shown by dot blot. Intracellular levels are shown by Western blot, DnaJC5 was detected by FLAG antibody. Quantification of extracellular tau levels is shown as average ± SEM, n=3, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "B. Chemical inhibition of Hsc70 activity in HEK293T cells overexpressing FLAG-DnaJC5 reduces extracellular α-synuclein levels as shown by dot blot. Intracellular levels are shown by Western blotDnaJC5 was detected by FLAG antibody. Quantification of extracellular synuclein levels is shown as average ± SEM, n=3, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "C. Dominant-negative Hsc70 E175S blocks tau release when overexpressed in HEK293T cells as shown by dot blot. Intracellular levels are shown by Western blot. Quantification of extracellular tau levels is shown as average ± SEM, n=4, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"Hsc70: 3312
tau: 4137"
Figure legend: "D. Knockdown of Hsc70 reduces extracellular tau release (dot blot) in HEK293T cells overexpressing FLAG-DnaJC5. Intracellular levels are shown by Western blot, DnaJC5 was detected by FLAG antibody. Quantification of extracellular tau levels is shown as average ± SEM, n=3, ***p&lt;0.001 one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130"
Figure legend: "E. A mutant tau that does not bind Hsc70 is not released when FLAG-DnaJC5 is overexpressed in HEK293T cells. Dot blot and quantification of extracellular tau levels are shown. Intracellular protein levels are shown with representative Western blot, DnaJC5 was detected by FLAG antibody. Data are mean ± SEM, n=3 p&lt;0.001. one-way analysis of variance with Tukey's multiple comparison post-hoc analysis."
NCBI gene IDs:
"DnaJC5: 79130
tau: 4137"
Figure legend: "H. Dot blot of increasing FLAG-DnaJC7 levels on FLAG-DnaJC5 overexpression-mediated extracellular tau release in HEK293T cells. Intracellular protein levels are shown with representative Western blot, DnaJ proteins were detected by FLAG antibody. Quantification of extracellular levels are mean ± SEM, n=4."
NCBI gene IDs:
"DnaJC5: 79130
DnaJC7: 7266"
Figure legend: "(B, Performance in the multi-trail inverted screen test  is impaired in (B) SUR2-STOP1149 (WT n = 9 [3 male; 6 female]; STOP n = 9 [3 male; 6 female]; p = 0.011 according to Mann Whitney U test) Data information: Bars show mean (± SEM) with individual biological replicates shown as circles/squares. * denotes p value &lt; α = 0.05 for all panels."
NCBI gene IDs:
"SUR2: 20928"