EMBO/SourceData huggingface dataset:
Figure legend: " Hela cells were co-transfected with an RFP-based TDP-NLS reporter and GFP or GA175-GFP. 24 h after transfection, cells were treated with rolipram (30 µM) for 16h. (C) RFP-NLSTDPWT mRNA expression levels were measured by qPCR. n=3 biological replicates. Bar-graphs of mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. "
NCBI gene IDs:
"GFP: 
RFP: 
GA: 203228
TDP: 23435"
Figure legend: " Hela cells were co-transfected with the RFP-based TDP-NLS reporter, GFP or GA175-GFP, as well as PSMD11 or empty vector. Image analysis as in (A). n=4 biological replicates. Scatter plot with bar-graphs of mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. 354 GFP and 330 GA175-GFP cells with vector, 367 GFP and 369 GA175-GFP cells with PSMD11 in total were analyzed. "
NCBI gene IDs:
"GFP: 
RFP: 
GA: 203228
PSMD11: 5717"
Figure legend: " Hela cells were co-transfected with the RFP-based TDP-NLS reporter, GFP or GA175-GFP, as well as PSMD11 or empty vector. F) RFP-NLSTDPWT mRNA expression levels were measured by qPCR. n=3 biological replicates. Bar-graphs of mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. "
NCBI gene IDs:
"GFP: 
RFP: 
GA: 203228
PSMD11: 5717
TDP: 23435"
Figure legend: " Immunofluorescence of Hela cells transfected with GFP or GA175-GFP showing reduced poly-GA aggregation upon rolipram treatment (30 µM, 16 h). (H) Automated quantification of poly-GA aggregate number per cell. n=3 biological replicates. In total 223 cells treated with vehicle and 286 cells treated with rolipram were analyzed. Scatter plot with bar-graphs of mean ± SD. Unpaired two-tailed t-test with Welch's correction. "
NCBI gene IDs:
"GFP: 
GA: 203228"
Figure legend: " (I) GA-GFP mRNA expression levels were measured by qPCR. n=3 biological replicates. Bar-graphs of mean ± SD. Unpaired two-tailed t-test with Welch's correction. "
NCBI gene IDs:
"GFP: 
GA: 203228"
Figure legend: " (B) Immunoblot of HeLa cells transfected with RFP-based TDP-NLS wild-type (WT) or mutants (K84A, K84R, K95A, K95R). "
NCBI gene IDs:
"RFP: 
TDP: 23435"
Figure legend: " (C HeLa cells were co-transfected with the indicated TDP-NLS reporters as well as GFP or GA175-GFP, and treated with MG132 (10µM) or vehicle for 16h. "
NCBI gene IDs:
"GFP: 
GA: 203228
TDP: 23435"
Figure legend: " (D) Automated quantification of RFP-NLS reporters in GFP positive cells. Note that K84A and K84R block overall import, while K95A and K95R allow import but are resistant to inhibition by poly-GA. n=4 biological replicates. The total number of cells analyzed per group was (from left to right): 667, 581, 789, 783, 809, 708, 628, 721, 938, 557, 857, 861, 886, 699,789, 539, 636, 577, 638 and 870. Scatter plot with bar-graphs of mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. *** denotes p&lt;0.001. Scale bar denotes 20 µm. "
NCBI gene IDs:
"GFP: 
RFP: 
poly-GA: 203228"
Figure legend: " (E,F) HeLa cells were co-transfected with the indicated GFP-TDP NLS reporters and iRFP or GA175-iRFP. Cell lysates were immunoprecipitated with anti-GFP and immunoblotted with indicated antibodies to detect co-immunoprecipitation of the TDP-43 NLS with importin-α5/KPNA1 nuclear import receptor. (F) Quantification of KPNA1 levels normalized to total GFP-NLSTDP reporter levels in anti-GFP immunoprecipitates. n=3 biological replicates. Scatter plot with mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. *** denotes p&lt;0.001. See also Figure EV4. Red dashed line indicates the control's expression level. "
NCBI gene IDs:
"GFP: 
iRFP: 
GA: 203228
TDP: 23435"
Figure legend: " HeLa cells were co-transfected with wildtype or K95A GFP-TDP NLS, HA-ubiquitin and iRFP or GA175-iRFP. 24h after transfection, cells were treated with rolipram (30 µM), MG132 (10 µM) or DMSO (vehicle) for 16h. Lysates were immunoprecipitated with Protein G beads coupled with anti-GFP antibody. (A) Immunoblotting of input (left panels) and anti-GFP immunoprecipitates (right panels) to show GFP-reporter levels and poly-ubiquitination. (B) Quantification of HA-ubiquitin levels normalized to total GFP- NLSTDP reporter levels in anti-GFP immunoprecipitates. n=3 biological replicates. Scatter plot, mean ± SD. One-way ANOVA with Tukey's multiple comparisons test. * denotes p&lt;0.05, and *** denotes p&lt;0.001. Red dashed line indicates the control's expression level. "
NCBI gene IDs:
"GFP: 
HA: 
iRFP: 
GA: 203228
TDP: 23435"
Figure legend: "B WB  showing depletion of PERK  in Perk  KO astrocytes  and neurons."
NCBI gene IDs:
"Perk: 13666"
Figure legend: "D-I eIF2α phosphorylation  in WT and Perk  KO astrocytes  (N=3) and neurons  (N=6) ± 20-24 hours of TM-induced ER  stress. Representative WB  (D, G), quantification of absolute phosphorylated eIF2α  level (p-eIF2α/eIF2α) (E, H) and the ER  stress-induced p-eIf2α  response (F, I). Data information: (E, H, Data are normalised to untreated WT. (F, I Data are related to untreated cells of the same genotype. Baseline level (without ER  stress) is depicted by a dashed line. Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: ** P&lt;0.01, *** P&lt;0.001, **** P&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (E, H, Students t-test (F, I)"
NCBI gene IDs:
"Perk: 13666"
Figure legend: "J-O Protein synthesis in WT and Perk  KO astrocytes  (N=5) and neurons  (N=5) ± 20-24 hours of TM-induced ER  stress. Representative images obtained by high-content microscopy  showing puromycinilated proteins detected by immunofluorescence  (J, M), quantification of puromycin  intensity as measure for de novo protein synthesis (K, N) and the ER  stress-induced protein synthesis response (L, O). Astrocytic soma  (GFAP, white), dendrites  (MAP2, white), nuclei  ((Δ)Cre, red), de novo synthesized proteins (Puromycin, green). Scale bar: 25 μm. Data information: K, N) Data are normalised to untreated WT. , L, O) Data are related to untreated cells of the same genotype. Baseline level (without ER  stress) is depicted by a dashed line. Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: ** P&lt;0.01, *** P&lt;0.001, **** P&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test K , N); Nested t-test (L, O)."
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "A-F ATF4 expression   in WT and Perk  KO astrocytes  (N=3) and neurons  (N=9) ± 20-24 hours of TM-induced ER  stress. (A, D) Representative confocal  images of ATF4 immunofluorescence. Astrocytic soma  (GFAP, cyan), dendrites  (MAP2, cyan), nuclei  ((Δ)Cre, green), ATF4  (magenta). Scale bar: 10 μm. (B, E) Quantification of absolute nuclear ATF4  intensity. Data are normalised to untreated WT. (C, F) Quantification of the ER  stress-induced ATF4 response. Data are related to untreated cells of the same genotype. Baseline level (without ER  stress) is depicted by a dashed line. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant p-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (B, E); Nested t-test (C, F)."
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "G-J (G, I) Venn diagram showing the number of significantly upregulated ATF4  target genes in WT (grey and blue) and Perk  KO (green and blue) astrocytes  (G, N=3) and neurons  (I, N=3) upon 20-24 hours of TM-induced ER  stress determined by mRNA-seq. Percentage: fraction of upregulated ATF4  target genes in WT cells that are also upregulated in Perk  KO cells. (H, N=3; J, N=3) Log2 fold change of ATF4  target genes that are significantly upregulated in both WT and Perk  KO cells (blue in Venn diagram). Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant p-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant."
NCBI gene IDs:
"Perk: 13666"
Figure legend: "Protein synthesis and ATF4 expression  in mouse  (A-F) astrocytes  (A-C and neurons  (D-F ± 20-24 hours of TM-induced ER  stress ± PERK-i (Pi). (A, D, Representative images obtained by  high-content microscopy. Astrocytic soma  (GFAP dendrites  (MAP2, white), nuclei  (ΔCre, red), de novo synthesized proteins (Puromycin, green), ATF4  (magenta). Scale bar: 25 μm. (B, N=5; E, N=5; Quantification of puromycin  intensity as measure for de novo protein synthesis. (C, N=5; F, N=7; Quantification of absolute nuclear ATF4  intensity. Data information: Data are normalised to untreated WT and presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *** p&lt;0.001, **** p&lt;0.0001. Statistical analysis: Two-way ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "Protein synthesis and ATF4 expression  in human  (G-L) astrocytes  G-I, ) and neurons  J-L) ± 20-24 hours of TM-induced ER  stress ± PERK-i (Pi). G, J) Representative images obtained by high-content microscopy. Astrocytic soma Phalloidin, white), dendrites  (MAP2, white), nuclei  (ΔCre, red), de novo synthesized proteins (Puromycin, green), ATF4  (magenta). Scale bar: 25 μm. ; H, N=3; K, N=3) Quantification of puromycin  intensity as measure for de novo protein synthesis. I, N=4; L, N=3) Quantification of absolute nuclear ATF4  intensity. Data information: Data are normalised to untreated WT and presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *** p&lt;0.001, **** p&lt;0.0001. Statistical analysis: Two-way ANOVA with Tukey's post hoc test."
NCBI gene IDs:
"Cre: 2777477"
Figure legend: "D-G ROS  accumulation in WT and Perk KO neurons (N=3) and astrocytes  (N=3) ± 20-24 hours of TM-induced ER  stress. (D, F) Representative images obtained by high-content microscopy. Dendrites   (MAP2, white), astrocytic soma  (Phalloidin, white), nuclei ((Δ)Cre, red), ROS  (CellROX  green, green). Scale bar: 25 μm. (E, G) Quantification of CellROX  green intensity. Data information: (E, G, Data are normalised to untreated WT. Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (E, G,"
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "H mRNA expression of Eif2ak1  (HRI) in HRI  KD neurons  determined by RT-qPCR, normalised to WT transduced with shRNA control (represented by a dashed line) (N=3). Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis One sample t-test with a hypothetical value of 100 (H"
NCBI gene IDs:
"Eif2ak1: 15467
HRI: 15467"
Figure legend: "I Quantification of the increase in nuclear ATF4  intensity in WT neurons  ± HRI  KD ± 20 hours of sodium arsenite  (Ars) treatment (N=3). J The remaining ATF4  response (%) upon HRI  KD, related to ATF4  response of WT transduced with shRNA control (100%, represented by a dashed line) (N=3). Calculated from data in (I): [[(KD_Ars+-KD_Ars-)/KD_Ars-]/[(WT_Ars+-WT_Ars-)/WT_Ars-]]*100. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test I, One sample t-test with a hypothetical value of 100 J)"
NCBI gene IDs:
"HRI: 15467"
Figure legend: "K-M eIF2α phosphorylation in HRI  KD WT and Perk  KO neurons (N=3) ± 20-24 hours of TM-induced ER  stress. Representative WB  (K), quantification of the ER  stress-induced p-eIf2α  response (L) and absolute phosphorylated eIF2α  level (p-eIF2α/eIF2α) (M). Data information: M, Data are normalised to untreated WT. (L, Data are related to untreated cells of the same genotype and normalised to WT without HRI  KD. Baseline level (without ER stress) is depicted by a dashed line. Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test L, Students t-test (M)"
NCBI gene IDs:
"HRI: 15467
Perk: 13666"
Figure legend: "N-S Protein synthesis (N=3) and ATF4 expression  (N=4) in HRI  KD WT and Perk  KO neurons  ± 20-24 hours of TM-induced ER  stress. Representative images obtained by (N) confocal  and (Q)  high-content microscopy. Dendrites  (MAP2, white/cyan), nuclei  ((Δ)Cre, green/red), de novo synthesized proteins (Puromycin, green), ATF4  (magenta). Scale bar: 25 μm. Quantification of the ER  stress-induced protein synthesis  response (O) and puromycin intensity as measure for de novo protein synthesis (P). Quantification of the ER  stress-induced ATF4  response (R) and absolute nuclear ATF4  intensity (S). Data information: P, S) Data are normalised to untreated WT. O, R) Data are related to untreated cells of the same genotype and normalised to WT without HRI  KD. Baseline level (without ER  stress) is depicted by a dashed line. Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test O, R); Nested t-test (P, S)."
NCBI gene IDs:
"Cre: 2777477
HRI: 15467
Perk: 13666"
Figure legend: "A-F Protein synthesis (A-C, N=3) and ATF4 expression  (D-F, N=5) in WT and Perk  KO neurons  overexpressing eIF2αWT or phospho-deficient eIF2αS51A ± 20-24 hours of TM-induced ER  stress. Representative images obtained by (A) confocal  and (D)  high-content microscopy. Dendrites  (MAP2, white/cyan), nuclei  ((Δ)Cre, red), de novo synthesized proteins (Puromycin, green), ATF4  (magenta). Scale bar: 25 μm. Quantification of the ER  stress-induced protein synthesis (B) and ATF4  response (E). Data are normalised to WT eIF2αWT. Quantification showing the percentage change in the ER  stress-induced protein synthesis (C) and ATF4  (F) response induced by eIF2αS51A compared to eIF2αWT. (C) Calculated from data in (B): [(eIF2αS51A-eIF2αWT)/eIF2αWT] *100. (F) Calculated from data in (E): [(eIF2αS51A-eIF2αWT)/eIF2αWT]*-100. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *** p&lt;0.001, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (B, E, Nested t-test (C, F)."
NCBI gene IDs:
"Cre: 2777477
eIF2α: 83939
Perk: 13666"
Figure legend: "Expression   of p-eIF2α  sensitive (5'ATF4-uORFWT) and p-eIf2α   insensitive (5'ATF4-uORFMut) EYFP translational reporters in WT and Perk  KO neurons  ± 20-24 hours of TM-induced ER  stress (N=3). (I, K) Representative confocal  images. Dendrites  (MAP2, cyan), nuclei  ((Δ)Cre, red), endogenous ATF4  (magenta), ATF4  reporter (green). Scale bar: 10 μm. (J, L) Quantification of EYFP  intensity, normalised to untreated WT. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *** p&lt;0.001, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test J, L)"
NCBI gene IDs:
"EYFP: 
ATF4: 468
Cre: 2777477
Perk: 13666"
Figure legend: "A-F  Protein synthesis and ATF4 expression  in ISRIB-treated WT and Perk  KO neurons  ± 20-24 hours of TM-induced ER  stress (N=4). (A, D) Representative images obtained by high-content microscopy. Dendrites  (MAP2, white), nuclei ((Δ)Cre, red), de novo synthesized proteins (Puromycin, green), ATF4  (magenta). Scale bar: 25 μm. Quantification of the ER  stress-induced protein synthesis (B) and ATF4  (E) response. Data are normalised to WT without ISRIB. Baseline level (without ER  stress) is depicted by dashed line. Quantification showing the percentage change in the ER  stress-induced protein synthesis (C) and ATF4  (F) response induced by ISRIB. (C) Calculated from data in (B): [(ISRIB+-ISRIB-)/ISRIB-]*100. (F) Calculated from data in (E): [(ISRIB+-ISRIB-)/ISRIB-]*-100. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, *** p&lt;0.001, **** p&lt;0.0001 and ns: not significant. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (B, E); Nested t-test (C, F)"
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "A Differentially expressed genes (DEGs) involved in oxidative stress related processes comparing TM-treated Perk  KO and WT neurons. Blue arrow: Ang."
NCBI gene IDs:
"Ang: 11727
Perk: 13666"
Figure legend: "B, C Ang  mRNA level in WT and Perk  KO neurons  and astrocytes  ± 20-24 hours of TM-induced ER  stress, determined by mRNA-seq (N=3). FPMK: Fragments Per Kilobase of transcript per Million. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001, **** p&lt;0.0001. Statistical analysis: Two-way ANOVA with Tukey's post hoc test (B, C,"
NCBI gene IDs:
"Ang: 11727
Perk: 13666"
Figure legend: "F, G tRNA  unfolding in WT and Perk  KO neurons  ± 20-24 hours of TM-induced ER  stress (N=4). (F) Representative images obtained by  high-content microscopy   of 1-methyladenosine (m1A) immunofluorescence  to determine tRNA  unfolding. Dendrites  (MAP2, white), nuclei  ((Δ)Cre, red), unfolded tRNA  (m1A, green). Scale bar: 25 μm. (G) Quantification of m1A  intensity, normalised to untreated WT. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001, **** p&lt;0.0001. Statistical analysis: Two-way ANOVA with Tukey's post hoc test G,"
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "H-M Abundance of small RNAs in WT and Perk  KO astrocytes  (N=3) and neurons  (N=6) ± 20-24 hours of TM-induced ER  stress, determined by high-resolution automated electrophoresis  (H, K) Representative gel image. Electropherogram  (I, L) and zoom (J, M) showing quantified fluorescence units (FU) of  small RNAs, size ranges of  tRNAs and tsncRNAs  are indicated. Shown is the mean of the biological replicates."
NCBI gene IDs:
"Perk: 13666"
Figure legend: "N-R Protein synthesis  and ATF4 expression  in WT and Perk  KO neurons  ± 20-24 hours of TM-induced ER  stress in the presence or absence of ANG-I (N=3). (N) Representative images obtained by  high-content microscopy. Dendrites  (MAP2, white), nuclei  ((Δ)Cre, red), de novo synthesis of proteins (Puromycin, green). Scale bar: 25 μm. Quantification of the ER  stress-induced protein synthesis (O) and ATF4  response (Q). Data are normalised to WT without ANG-i. Baseline level (without ER  stress) is depicted by dashed line. Quantification showing the percentage change in the ER  stress-induced protein synthesis (P) and ATF4  response (R) induced by ANG-i. (P) Calculated from data in (O): [(ANG-i+-ANG-i-)/ANG-i-]*100. (R) Calculated from data in (Q): [(ANG-i+-ANG-i-)/ANG-i-]*-100. Data information: Data are presented as mean ± SEM. N: Biological replicate. Relevant P-values are indicated: *p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001, **** p&lt;0.0001. Statistical analysis: Two-way ANOVA with Tukey's post hoc test O, Q); Nested t-test (P, R)."
NCBI gene IDs:
"Cre: 2777477
Perk: 13666"
Figure legend: "A  PHIP-1 interaction  with GPB-1 by  yeast two-hybrid assay. The reporter strain PJ69-4A was co-transformed with expression vectors encoding GAL4  DBD-PHIP-1(H45A) and GAL4  AD-GPB-1, as indicated. Yeast  strains carrying the indicated plasmids were cultured on a selective plate lacking histidine  and containing 5 mM 5-aminotriazole for 4 days."
NCBI gene IDs:
"GAL4: 855828
GPB-1: 174803
PHIP-1: 185336"
Figure legend: "A His-phosphorylation  of GPB-1 in animals. The  phip-1(km96) mutant animals carrying the 3XFLAG::gpb-1 or 3XFLAG::gpb-1(H266F) knock-in allele were lysed. The lysates were treated with or without heating  (95°C) and immunoblotted  (IB) with anti-3-pHis and anti-FLAG  antibodies."
NCBI gene IDs:
"FLAG: 
gpb-1: 174803
phip-1: 185336"
Figure legend: "B  NDK-1 phosphorylates GPB-1 in vitro. COS-7 cells were co-transfected with HA-GPB-1 or HA-GPB-1(H266F) and T7-GPC-2, and cell lysates were immunoprecipitated  (IP) with anti-HA  antibodies. Immunopurified GPB-1 was subjected to the in vitro  kinase assay with recombinant GST-NDK-1. Phosphorylated GPB-1  was detected by immunoblotting  (IB) with anti-3-pHis antibodies."
NCBI gene IDs:
"HA: 
T7: 
GPB-1: 174803
GPC-2: 172396"
Figure legend: "C PHIP-1 dephosphorylates GPB-1 in vitro. COS-7 cells were co-transfected with HA-GPB-1 and T7-GPC-2, and cell lysates were immunoprecipitated  (IP) with anti-HA  antibodies. The immunopurified HA-GPB-1 was first subjected to the in vitro  kinase assay  with recombinant GST-NDK-1. Phosphorylated GPB-1 was then equally aliquoted and subjected to the in vitro phosphatase assay  with recombinant GST-PHIP-1 or its variants. Phosphorylated GPB-1 was detected by immunoblotting  (IB) with anti-3-pHis antibodies."
NCBI gene IDs:
"HA: 
GPB-1: 174803
GPC-2: 172396"
Figure legend: "A  PHIP-1 interaction  with   UNC-51  by yeast two-hybrid assay. The reporter strain PJ69-4A was co-transformed with expression vectors encoding GAL4  DBD-PHIP-1(H45A) and GAL4  AD-UNC-51(274-856), as indicated. Yeast  strains carrying the indicated plasmids were cultured on a selective plate lacking histidine  and containing 5 mM 5-aminotriazole for 4 days."
NCBI gene IDs:
"GAL4: 855828
PHIP-1: 185336
UNC-51: 180311"
Figure legend: "C UNC-51 phosphorylates PHIP-1 at Ser-112. COS-7  cells were co-transfected with Flag-PHIP-1 (WT or mutants) and GFP-UNC-51 [WT or ∆AIKAI (KD)], and cell lysates were analyzed using Phos-tag  SDS-PAGE. Total lysates were immunoblotted  (IB) with antibodies, as indicated. Filled and open arrowheads indicate unmodified and phosphorylated PHIP-1, respectively. Asterisk indicates non-specific band."
NCBI gene IDs:
"Flag: 
GFP: 
PHIP-1: 185336
UNC-51: 180311"
Figure legend: "C) Representative cases show the first and fourth generation xenograft H&amp;E staining (left) and EBER-1 ISH staining (right)."
NCBI gene IDs:
"EBER-1: "
Figure legend: "Expression of stemness markers (Sox2, Klf4, Bmi1, Oct4) and drug resistance genes (ABCG2, Mrp1) evaluated by real-time PCR (G) in SNU719 and SNU-4th cells."
NCBI gene IDs:
"Mrp1: 4363
ABCG2: 9429
Bmi1: 648
Klf4: 9314
Oct4: 5460
Sox2: 6657"
Figure legend: "I) Real-time PCR analysis of the expression of EMT-associated markers (E-cad, vimentin) and transcription factors (Twist1, Snail, Slug, Zeb1) in SNU719 and SNU-4th cells."
NCBI gene IDs:
"E-cad: 999
Snail: 6615
Slug: 6591
Twist1: 7291
vimentin: 7431
Zeb1: 6935"
Figure legend: "(B) Real-time PCR analysis of the expression of circLMP2A in SNU-4th, SNU719 and YCCEL1 cells."
NCBI gene IDs:
"circLMP2A: "
Figure legend: "(C) The existence of circLMP2A was validated by RT-PCR (up) and real-time PCR (down) in SNU-4th, SNU719 and YCCEL1 cells treated with or without RNase R digestion. GAPDH was used as a negative control."
NCBI gene IDs:
"circLMP2A: 
GAPDH: "
Figure legend: "(D) Real-time PCR analysis of circLMP2A and LMP2A expression after treatment with actinomycin D at the indicated time points in SNU-4th cells."
NCBI gene IDs:
"circLMP2A: 
LMP2A: 3783751"
Figure legend: "(E) Real-time PCR analysis of circLMP2A and LMP2A expression in either the cytoplasm or nucleus in SNU-4th cells. GAPDH and U6 were used as controls in cytoplasm and nucleus, respectively."
NCBI gene IDs:
"circLMP2A: 
GAPDH: 
U6: 
LMP2A: 3783751"
Figure legend: "(F) RNA ISH for circLMP2A. U6 and 18S are mainly localized in the nucleus and cytoplasm, respectively, and were used as controls."
NCBI gene IDs:
"18S: 
circLMP2A: 
U6: "
Figure legend: "(G) Kaplan-Meier survival curve analysis showing the correlation between circLMP2A expression and OS. n= 69, P &lt; 0.001, log-rank test. (H) Kaplan-Meier survival curve analysis showing the correlation between circLMP2A expression and progression-free survival (PFS). n = 69, P &lt; 0.001, log-rank test. "
NCBI gene IDs:
"circLMP2A: "