EMBO/SourceData huggingface dataset:
Figure legend: "THP-1-derived ANG translocating into HCT116KO through PLXNB2 in co-cultured system as showed by western blotting. Neamine, a blocker of ANG and PLXNB2 interaction; siCtrl: control siRNA; siPLXNB2-1 and siPLXNB2-2"
NCBI gene IDs:
"PLXNB2: 23654"
Figure legend: "tiRNA and 5'-tiRNAAla production in HCT116KO following different treatments as indicated. ANGR66A, ANG receptor binding site variant; ANGK40I, ANG enzymatic activity null variant; BAY, inhibitor of TNF-α-induced κB kinase (IKK) phosphorylation"
NCBI gene IDs:
"5'-tiRNAAla: 
tiRNA: "
Figure legend: "The effect of 5'-tiRNA knockdown or overexpression on TNF-α-induced apoptosis in ANGWT- or ANGK40I-treated HCT116KO. si5'-tiRNA: tiRNA-targeting siRNA"
NCBI gene IDs:
"5'-tiRNA: 
tiRNA: "
Figure legend: "The interaction between 5'-tiRNA and cytochrome c (Cyt c) in HCT116 cells as detected by RIP."
NCBI gene IDs:
"5'-tiRNA: "
Figure legend: "Cell viability of HCT116KO following different treatments as indicated. siCtrl: control siRNA; siPLXNB2-1 and siPLXNB2-2: PLXNB2 siRNAs"
NCBI gene IDs:
"PLXNB2: 23654"
Figure legend: "Detection of 47S pre-rRNA transcription level with northern blotting in HCT116KO following different treatments as indicated. siPLXNB2-1 and siPLXNB2-2: PLXNB2 siRNAs"
NCBI gene IDs:
"47S pre-rRNA: 
PLXNB2: 23654"
Figure legend: "tiRNA and 5'-tiRNAAla production in IECs isolated from WT and Ang-/- colitis mice at indicated time point."
NCBI gene IDs:
"5'-tiRNAAla: 
tiRNA: 
Ang: 11727"
Figure legend: "47S pre-rRNA transcription level in IECs from WT and Ang-/- colitis mice at indicated time point."
NCBI gene IDs:
"47S pre-rRNA: 
Ang: 11727"
Figure legend: "(A) BECN1 expression of microglia from Becn1+/- and wild type mouse pups in full medium without Bafilomycin A1 (Baf) was quantified by Western blotting. Microglia from Becn1+/- mice show a signifcant reduction of BECN1 (48%) compared to microglia from wild type mice; mean ± SEM, wild type n = 3, Becn1+/- n = 7; two-tailed t-test **: p &lt; 0.01. Blot shows representative amounts of BECN1 after various treatments."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(B) Microglia from newborn Becn1+/- and wild type mouse pups were kept for 2 h either in full medium or HBSS with or without Bafilomycin A1 (Baf). LC3 and ACTIN levels were determined by Western blot. LC3-II was significantly reduced in microglia from Becn1+/- mice after Baf treatment in full medium compared to wild type microglia; mean ± SEM, wild type n = 3, Becn1+/- n = 4; two-tailed t-test *: p &lt; 0.05."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(C) Microglia from newborn Becn1+/- and wild type mouse pups were kept for 2 h either in full medium or HBSS with Bafilomycin A1 (Baf) and stained for endogenous LC3. Exemplary images and the quantification of LC3-positive vesicles/µm2 cell area show reduced presence of LC3-positive vesicles in microglia from Becn1+/- and induction of autophagy by HBSS; mean ± SEM, wild type n = 4, Becn1+/- n = 4, 3-5 fields per view/n and condition and 73-271 cells/n and condition; two-tailed t-test *: p &lt; 0.05; scale bar: 20 µm"
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(A, B) Microglia isolated from newborn Becn1+/- and wild type mouse pups were either non treated or treated with a pro-inflammatory stimulus (LPS followed by ATP). As additional controls cells treated only with LPS or ATP were used in (A). The concentration of the pro-inflammatory cytokines IL-1beta (A) and IL-18 (B) in the cell supernatant was determined by ELISA. Reduction of BECN1 significantly enhanced release of both cytokines after stimulation with LPS/ATP; mean ± SEM, wild type n (A/B) = 9/2, wild type LPS n = 3/0, wild type ATP n = 3/0, wild type LPS/ATP n = 9/9, Becn1+/- n = 23/5, Becn1+/- LPS/ATP n = 23/23, two-tailed t-test *: p &lt; 0.05."
NCBI gene IDs:
"Becn1: 56208
BECN1: 56208"
Figure legend: "(E) Proteins were extracted from brains of wild type, Becn1+/-, APPPS1 and APPPS1 Becn1+/- mice at the indicated ages. IL-1beta was measured by electrochemiluminescence (MesoScale) and compared within the repective age groups. IL-1beta was significantly increased in the TBS and TX fraction; mean ± SEM, wild type: n (per age group) = 2/6/3, Becn1+/-: n = 2/5/2, APPPS1: n = 5/5/6, APPPS1 Becn1+/-: n = 7/9/6; ANOVA with Dunnett´s post hoc test with WT as control group; *: p &lt; 0.05; **: p &lt; 0.01."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(F) Proteins were extracted from brains of APPPS1 and of APPPS1 Becn1+/- mice at the age as indicated. Amyloid beta 1-40 and 1-42 were measured by electrochemiluminescence (MesoScale) and compared within the repective age groups. No difference in the amyloid beta load between the genotypes is apparent; mean ± SEM, APPPS1: n = 5/5/6, APPPS1 Becn1+/-: n = 7/9/6; ANOVA with Dunnett´s post hoc test with APPPS1 as control group, ns."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(A) The expression of NLRP3 protein in non treated and LPS/ATP treated wild type and Becn1+/- microglia was determined by Western blot and normalised to ACTIN. Reduction of BECN1 significantly enhanced expression of NLRP3; mean ± SEM, wild type n = 4, wild type LPS/ATP n = 9, Becn1+/- n = 4, Becn1+/- LPS/ATP n = 23; ANOVA with Tukey´s post hoc test *: p &lt; 0.05."
NCBI gene IDs:
"Becn1: 56208
BECN1: 56208"
Figure legend: "(B) The expression of Nlrp3 mRNA in non treated and LPS/ATP treated wild type and Becn1+/- microglia was determined by qPCR. No significant differences can be detected between wild type and Becn1+/- microglia; mean ± SEM, wild type: n = 7, Becn1+/- : n = 3; ANOVA with Tukey ´s post hoc test, ns for LPS/ATP treated wild type versus Becn1+/-."
NCBI gene IDs:
"Becn1: 56208
Nlrp3: 216799"
Figure legend: "(C) LPS/ATP treated and non treated wild type and Becn1+/- microglia were immunolabeled for ASC (red) and imaged by confocal microscopy to investigate the assembly of inflammasomes. In non stimulated cells ASC is distributed diffusely in the cytoplasm (upper panels). However, after stimulation formation of inflammasomes (arrowheads) can be detected in a subpopulation of cells as well as release of inflammasomes after pyroptosis/ejection (arrow). Quantification showed a significantly increased percentage of inflammasomes in or around Becn1+/- microglia; mean ± SEM, wild type LPS/ATP n = 4, Becn1+/- LPS/ATP n = 7; two-tailed t-test *: p &lt; 0.05; scale bar: 50 µm, insert scale bar: 10 µm."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(D) The presence of cleaved CASP1 (p10) protein in the supernatant of LPS/ATP treated wild type and Becn1+/- microglia was determined by Western blot and normalised to ACTIN. Reduction of BECN1 significantly enhanced release of CASP1; mean ± SEM, wild type LPS/ATP n = 8, Becn1+/- LPS/ATP n = 18; two-tailed t-test *: p &lt; 0.05."
NCBI gene IDs:
"Becn1: 56208
BECN1: 56208"
Figure legend: "(A) LPS/ATP treated and non treated wild type and Becn1+/- microglia were immunolabeled for NLRP3 (green) and LC3 (red) and imaged by confocal microscopy. Stimulation resulted in the appearance of many NLRP3-containing aggregates of different sizes (arrows). In non treated cells, only a few overlapping signals with LC3-stained autophagosomes (arrowheads) are visible. Stimulated microglia however, showed multiple colocalizations of LC3 positive vesicles and NLRP3 aggregates; scale bar: 7.5 µm."
NCBI gene IDs:
"Becn1: 56208"
Figure legend: "(E) Microglia from Becn1+/- mice were transfected with scrambled siRNA or with siRNA for CALCOCO2. 6 d after transfection cells were stimulated with LPS/ATP. The expression of CALCOCO2 protein was determined by Western blot. Application of CALCOCO2 siRNA resulted in significantly reduced expression of CALCOCO2; mean ± SEM, n = 3; two-tailed t-test *: p &lt; 0.05."
NCBI gene IDs:
"Becn1: 56208
CALCOCO2: 76815"
Figure legend: "(F) The amount of IL-1beta in the supernatant released from the cells described in (E) was determined by ELISA. Microglia with a CALCOCO2 knockdown released significantly more IL-1beta; mean ± SEM, n = 3; two-tailed t-test *: p &lt; 0.05."
NCBI gene IDs:
"CALCOCO2: 76815"
Figure legend: "D Representative EM images of pep4Δ and pep4Δdrs2Δ strains. Black squares show a zoom-in in the vacuole. White arrowheads point to Cvt bodies."
NCBI gene IDs:
"drs2: 851207
pep4: 855949"
Figure legend: "E Representative CLEM images of drs2Δ cells. Ape1-GFP (top) correlates with a membrane-free ribosome exclusion area (middle). Black square (bottom) shows a zoom-in at the position correlating with Ape1-GFP (white arrowhead)."
NCBI gene IDs:
"drs2: 851207"
Figure legend: "Ape1 processing was analyzed by western blot in the indicated strains, and normalized to the processing achieved in cells expressing wild-type Drs2 ± SD, n ≥ 3 biological replicates."
NCBI gene IDs:
"Drs2: 851207"
Figure legend: "D Ape1 processing was analyzed by western blot in the indicated strains, and normalized to the processing achieved in cells expressing wild-type Drs2 ± SD, n = 3 biological replicates."
NCBI gene IDs:
"Drs2: 851207"
Figure legend: "A Ape1 processing was analyzed by  western blot  in cells overexpressing N-terminal Drs2  (1-212 aa) and normalized to the processing achieved in cells with an empty vector ± SD, n = 3."
NCBI gene IDs:
"Drs2: 851207"
Figure legend: "A Histograms representing the mean speed of Atg9 puncta observed in wild-type, drs2Δ and drs2-5A cells. The histograms were fitted to a mixture of Gaussian distributions with two components; the percentage of each population and the means of the fitting curves are indicated on top of the plot."
NCBI gene IDs:
"drs2: 851207"
Figure legend: "B Representative images (left) and quantification (right) of the co-localization between Atg9-GFP and Ape1-mCherry (upper and middle row, respectively) in cells expressing wild-type Drs2 or drs2-5A. White arrowheads point to Ape1-mCherry clusters co-localizing with Atg9-GFP. C Values were normalized to the measurements in cells expressing wild-type Drs2. Error bars: mean ± SD, n = 3 biological replicates. Asterisks indicate significant difference as determined by a two-tailed Student's t-test (**P&lt;0.01). Scale bar, 5µm."
NCBI gene IDs:
"Drs2: 851207
drs2: 851207"
Figure legend: "C Representative images (left) and quantification (right) of the PICT assay for the interaction of Trs85-FRB (TRAPPIII) and Atg9-GFP in cells expressing wild-type Drs2 or drs2-5A after adding rapamycin (RAP). RFP-tagged anchor and GFP-tagged prey are shown in the upper and middle row, respectively. Bottom row shows the merged images. White zoom in boxes, 0.9µm. , C Values were normalized to the measurements in cells expressing wild-type Drs2. Error bars: mean ± SD, n = 3 biological replicates. Asterisks indicate significant difference as determined by a two-tailed Student's t-test (**P&lt;0.01). Scale bar, 5µm."
NCBI gene IDs:
"Drs2: 851207
drs2: 851207"
Figure legend: "Precursor API is trapped in a prevacuolar compartment in vps18 cells. (A) Yeast strain JSR18Δ1 with plasmid pJSR9 (vps18 ts) was grown at the permissive temperature of 26°C. Before labeling, the cells were incubated at either 26° or 38°C for 10 min. Pulse labeling was for 5 min, followed by the indicated chase times."
NCBI gene IDs:
"vps18: 850840"
Figure legend: "(B) vps18 spheroplasts were shifted to 38°C for 5 min, pulse labeled for 5 min, and then chased for either 0 or 60 min. At each time point the spheroplasts were collected by centrifugation and resuspended in PS0 buffer. The Total (T) fraction either received no treatment, was treated with 50 μg/ml proteinase K, or received proteinase K in the presence of 0.2% Triton X-100. The supernatant (S0) and pellet (P0) fractions were collected after centrifugation at 10,000 g for 5 min."
NCBI gene IDs:
"vps18: 850840"
Figure legend: "A mutant in the API propeptide causes accumulation of prAPI on a nonvacuolar compartment. (A) Spheroplasts from an ape1Δ strain containing a plasmid bearing P22L API were subjected to differential lysis in PS200 buffer. The Total (T) fraction (after differential lysis) either received no treatment, was treated with 50 μg/ ml proteinase K, or received proteinase K in the presence of 0.2% Triton X-100."
NCBI gene IDs:
"ape1: 853758"
Figure legend: "(C) ape1Δ cells containing P22L API on a plasmid were pulse labeled for 5 min, harvested by centrifugation, and subjected to a nonradioactive chase in either nitrogen-containing (SMD) or nitrogen starvation (SD-N) medium. Samples were collected at the times indicated and immunoprecipitated with API antiserum. The resulting SDS-PAGE gels were quantified using a Molecular Dynamics STORM phosphorimager."
NCBI gene IDs:
"ape1: 853758"
Figure legend: "Precursor API is trapped within a vesicle in cvt17 mutants. (A) Precursor API is in a protease-protected compartment in cvt17 mutants. THY32 (cvt17) spheroplasts were subjected to differential lysis and protease treatment in PS200 buffer as in Fig 3"
NCBI gene IDs:
"cvt17: 850432"
Figure legend: "B) Precursor API is not free in the vacuolar lumen in cvt17 mutants. Differential fractionation was continued by resuspending the P200 fraction in PS0 buffer and separating the supernatant (S0) and pellet (P0) fractions by centrifugation at 10,000 g. The S0 and P0 fractions were treated with proteinase K in the presence and absence of Triton X-100 as indicated. All fractions were precipitated with 10% TCA and resolved by SDS PAGE; proteins of interest were detected by Western blotting. The positions of prAPI, mAPI, and mPrA are indicated."
NCBI gene IDs:
"cvt17: 850432"
Figure legend: "Precursor API is contained within subvacuolar vesicles in cvt17 mutants. (A) Isolated vacuoles (V) from cvt17 cells were lysed in PS0 buffer, loaded on top of an Optiprep step gradient, and centrifuged at 170,000 g for 60 min as described in Materials and Methods. Fractions VV, 0/1.06 interface; LD, 1.06 region; SV, 1.06/1.12 interface; HD, 1.12 region."
NCBI gene IDs:
"cvt17: 850432"
Figure legend: "Immuno-EM of cvt17 cells. Yeast strains SEY6210 (parental wild type) and THY32 (cvt17) were grown in YPD and prepared for microscopy as described in Materials and Methods. (A) Wild-type cells probed with antibody against mature API. (B) A cvt17 section showing a cytosolic vesicle containing prAPI probed with antibody against the API proregion. (C) A cvt17 section showing subvacuolar vesicles containing API probed with antibody against mature API. N, nucleus; V, vacuole; (arrows) API containing vesicles. Bars, 0.5 μm."
NCBI gene IDs:
"cvt17: 850432"
Figure legend: "(B). Relative values of the decreased phosphatase activities of different PTP-MEG2 mutants towards pNPP (p-Nitrophenyl phosphate) compared with wild-type PTP-MEG2. Data information: *** P&lt;0.001: PTP-MEG2 mutants compared with the control group. Data were obtained from 3 independent experiments. N.S. means no significant difference. All the data were analyzed using one-way ANOVA, and displayed as the mean ± s.e.m. In the red column indicates that the mutation sites have greater effects on the enzyme activities toward phospho-NSF segement than the pNPP. The green column indicates that the mutation sites only have significant effects on the enzyme activity toward NSF phospho-segement but not pNPP. The white column stands for the mutants that have similar effects on pNPP and NSF phospho-segement."
NCBI gene IDs:
"PTP-MEG2: 56294"
Figure legend: "(C). Fold-change decreases in the phosphatase activities of different PTP-MEG2 mutants towards the NSF-pY83 phospho-segment compared with wild-type PTP-MEG2. Data information: *** P&lt;0.001: PTP-MEG2 mutants compared with the control group. Data were obtained from 3 independent experiments. N.S. means no significant difference. All the data were analyzed using one-way ANOVA, and displayed as the mean ± s.e.m. In the red column indicates that the mutation sites have greater effects on the enzyme activities toward phospho-NSF segement than the pNPP. The green column indicates that the mutation sites only have significant effects on the enzyme activity toward NSF phospho-segement but not pNPP. The white column stands for the mutants that have similar effects on pNPP and NSF phospho-segement."
NCBI gene IDs:
"PTP-MEG2: 56294"
Figure legend: "(D). Statistical diagram of the quantal size in Fig. 5A and Appendix Figure S6A-D. The secretion amount of each group was standardized with respect to the control group. Data information: Green color represents the amino acids which are found to modulate foot probability based on structure analysis, and red color means amino acids which does not regulate foot probability. , * indicates PTP-MEG2 mutants overexpression group compared with the WT overexpression group. # indicates the overexpression group compared with the control group. **, P&lt;0.01; *** P&lt;0.001 and #, P&lt;0.05; ##, P&lt;0.01; ### P&lt;0.001. N.S. means no significant difference. Data were from 6 independent experiments. All the data were analyzed using one-way ANOVA and showed as the mean ± s.e.m."
NCBI gene IDs:
"PTP-MEG2: 56294"
Figure legend: "(C). The GST pull-down assay suggested that PACSIN1, MUNC18-1, VAMP7, SNAP25, DYNAMIN1 and DYNAMIN2 directly interact with PTP-MEG2. PC12 cells were transfected with plasmids of candidate substrates, including SYN1, MUNC18-3, PACSIN1, SCAMP1, MUNC18-1, PPP3CA, STX17, VAMP7, SYT7, SYT11, SNAP25, DYNAMIN1, and DYNAMIN2 stimulated with 100 nM AngII. The tyrosine phosphorylation of these proteins was verified by specific anti-pY antibodies (Appendix Figure S8A-E). The potential substrates of PTP-MEG2 in cell lysates were pulled down with a GST-PTP-MEG2-D470A trapping mutant and then detected by Western blot. White arrow stands for Western blot band consistent with the predicted molecular weight of the potential PTP-MEG2 substrate."
NCBI gene IDs:
"PTP-MEG2: 266611"
Figure legend: "(D). Interactions of the PTP-MEG2-trapping mutants with the MUNC18-1-Y145 mutants and DYNAMIN2-Y125 mutants. PC12 cells were transfected with FLAG-MUNC18-1-Y145, FLAG-DYNAMIN2-Y125 and different mutations of the FLAG-MUNC18-1-Y145A, Y145H, Y145E or Y145F, FLAG-DYNAMIN2-Y125A, Y125E, Y125F, 24 hours before stimulation with 100 nM AngII, respectively. The cell lysates were then incubated with GST-beads-PTP-MEG2-D470A complex for 2 hours with constant rotation. The potential PTP-MEG2 substrates were pulled down by GST-beads, and their levels were examined by the FLAG antibody with Western blot."
NCBI gene IDs:
"FLAG: 
DYNAMIN2: 1785
MUNC18: 6812"
Figure legend: "(H). The MUNC18-1-Y145 mutations decreased the interaction between MUNC18-1 and SYNTAXIN1. PC12 cells were transfected with plasmid encoding SYNTAXIN-1. The proteins in cell lysates were pulled down with purified GST-beads-MUNC18-1-Y145 complex and the GST-MUNC18-1-Y145H/E/F, and detected with SYNTAXIN1 antibody. The right histogram shows the quantified protein levels. Data information * indicates MUNC18-1, mutants overexpression group compared with the WT overexpression group. # indicates MUNC18-1, overexpression group compared with the control group. *, P&lt;0.05; **, P&lt;0.01; *** P&lt;0.001 N.S. means no significant difference. Data were from 3 (H) independent experiments. All the data were analyzed using one-way ANOVA, and showed as the mean ± s.e.m."
NCBI gene IDs:
"SYNTAXIN-1: 6804
MUNC18: 6812"
Figure legend: "(E). Relative phosphatase activities of different PTP-MEG2 mutants towards the MUNC18-1-pY145 phospho-segment compared with wild-type PTP-MEG2. (F). Relative phosphatase activities of different PTP-MEG2 mutants towards the DYNAMIN 2-pY125 phospho-segment compared with wild-type PTP-MEG2.Data information: (E, F) Residues are coloured according to Figure 8B. (E, F), * P&lt;0.05, ** P&lt;0.01, *** P&lt;0.001: PTP-MEG2 mutants compared with the control. N.S. means no significant difference. Data were obtained from 3 independent experiments. **, P&lt;0.01; *** P&lt;0.001 and ## P&lt;0.01; ### P&lt;0.001. N.S. means no significant difference. Data were obtained from 10 cells in each group of 6 independent experiments. . All the data were analyzed using one-way ANOVA and showed as the mean ± s.e.m."
NCBI gene IDs:
"PTP-MEG2: 266611"
Figure legend: "(B) Representative time-course of 2 mmol/L phosphate on calcification of LmnaG609G/+ VSMCs (up). Calcification was visualized with Alizarin red. Representative microscopic images (10x; scale bar: 100 µm) showing calcification of treated and untreated LmnaG609G/+ VSMCs (down)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(C-F) Measures of calcium in treated living LmnaG609G/+ VSMCs (C), treated fixed LmnaG609G/+ VSMCs (D), untreated living LmnaG609G/+ VSMCs (E), and untreated fixed LmnaG609G/+ VSMCs (F)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(A) Magnesium intake in 34-week-old untreated and treated LmnaG609G/+ mice (n=16)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(B) Plasma magnesium concentration in 34-week-old untreated and treated LmnaG609G/+ mice (n=16)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(C) Calcium content of aortas obtained from 34-week-old wild-type mice and untreated and treated LmnaG609G/+ mice (n=20)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(D) Body masses of 34-week-old untreated and treated LmnaG609G/+ mice."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(E) Kaplan-Meier graph for untreated and treated LmnaG609G/+ mice (n=16)."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(F) Representative photographs of 40-wk-old wild-type, untreated and treated LmnaG609G/+ mice."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "(A) ATP concentration in liver homogenates obtained from 34-week-old wild-type, untreated or treated LmnaG609G/+ mice."
NCBI gene IDs:
"Lmna: 16905"
Figure legend: "A IMR90 ER:RasV12 primary fibroblasts were cultured in the presence of EtOH  (proliferating) or 100nM 4OHT  (for 6-8days to induce RasV12 expression and oncogene-induced senescence, OIS). mRNA levels of 4OHT  cells, normalised to PUM1  and presented relative to EtOH  control. (n=4 independent experimental repeats). Data information: All graphs show individual data points, mean and error bars represent standard deviation. All data are analysed by 2-tailed, nonpaired Student's t-test (* &lt;0.05, ** &lt;0.01, *** &lt;0.001)"
NCBI gene IDs:
"ER: 2099
Ras: 3265
PUM1: 9698"
Figure legend: "A Fibroblasts  stably expressing GFP-TFEB  were treated 4OHT  to induce senescence (or EtOH  control). Cells were lysed and subject to  Western blotting for antibodies as indicated. B Quantification of A. (n=4 independent experimental repeats) C Quantification of A. (n=3 independent experimental repeats) Data information: All graphs show individual data points, mean and error bars represent standard deviation. B and C: analysed by 2-tailed, unpaired T-Test * p&lt;0.05; ** p&lt;0.01; *** p&lt;0.001."
NCBI gene IDs:
"GFP: 
TFEB: 7942"
Figure legend: "D Western blot of GFP immunoprecipitation  of control (EtOH) and senescent (4OHT) cells expressing GFP  or GFP-CDK4  and FLAG-TFEB."
NCBI gene IDs:
"FLAG: 
GFP: 
CDK4: 1019
TFEB: 7942"
Figure legend: "H Fibroblasts  stably expressing GFP-TFEB  were transduced with p16  shRNA simultaneously with induction of senescence by 4OHT. Cells were lysed and subject to Western blotting. I Quantification of H (n=4 independent experimental repeats). Data information: All graphs show individual data points, mean and error bars represent standard deviation. All other panels analysed by one-way ANOVA with Tukey's multiple comparison test (* &lt;0.05, ** &lt;0.01, *** &lt;0.001)"
NCBI gene IDs:
"GFP: 
p16: 1029
TFEB: 7942"
Figure legend: "J Fibroblasts   were transduced with p16  shRNA simultaneously with induction of senescence by 4OHT. Cells were lysed and subject to Western blotting. K, L Quantification of J (n=3 independent experimental repeats) Data information: All graphs show individual data points, mean and error bars represent standard deviation. All other panels analysed by one-way ANOVA with Tukey's multiple comparison test (* &lt;0.05, ** &lt;0.01, *** &lt;0.001)"
NCBI gene IDs:
"p16: 1029"
Figure legend: "M Fibroblasts  were transduced with p16  shRNA simultaneously with induction of senescence  by 4OHT. Cells were fixed and immunostained  with antibodies against p62  and Lamp2, Scale bar: 20µm."
NCBI gene IDs:
"p16: 1029"