EMBO/SourceData huggingface dataset:
Figure legend: "(A , B) A brightfield image showing the zebrafish thyroid gland along with surrounding tissue. The thyroid follicles reside in the soft tissue surrounding the ventral aorta, which extends from the outflow tract of the heart into the gills towards the basibranchial cartilage in the lower jaw. The thyroid follicular cells, or thyrocytes, are labeled in green in the Tg(tg:nls-mVenus-NTR) transgenic line (B'). Scale bars: 500 µm"
NCBI gene IDs:
"mVenus: 
tg: 567631"
Figure legend: "(C) Maximum intensity projection of 3D confocal stack obtained from the dissected thyroid gland of Tg(tg:nls-EGFP) animal and labeled with DAPI. (D) Confocal scan of a transverse section across the dissected thyroid gland from Tg(tg:nls-EGFP) animal at 3 mpf. Sections were stained with DAPI to visualize cells surrounding thyroid follicles. (E) Boxplot depicting the proportion of thyrocytes present in transverse sections obtained from three Tg(tg:nls-EGFP) animals at 3 mpf. Each dot represents a transverse section. The Tukey boxplot marks the 25th percentile, median and 75th percentile with whiskers extending from smallest to largest values. "
NCBI gene IDs:
"EGFP: 
tg: 567631"
Figure legend: "(F , G) Representative FACS plot of single cells from Tg(tg:nls-mVenus-T2A-NTR) animals at 2 mpf (F) and 8 mpf (G). Calcein (Pacific Blue) labels live cells, while green fluorescence (FITC) labels thyrocytes. Percentage values represent proportion of calcein+ thyrocytes within total calcein+ cells."
NCBI gene IDs:
"mVenus: 
tg: 567631"
Figure legend: "(A) A t-SNE plot displaying the 6249 single-cells profiled in the zebrafish thyroid gland atlas. The colors represent cell clusters denoting a specific cell-type. (B-D) Cluster #1 represents the thyrocytes that express tg, slc5a5 (NIS) and tpo. The color scale represents the normalized expression counts for each gene ranging from lowest (grey) to highest (red). "
NCBI gene IDs:
"slc5a5: 561445
NIS: 561445
tg: 567631
tpo: 569363"
Figure legend: "(A-D) Images show immunofluorescence labeling of thyroid gland from adult zebrafish. Transverse sections were utilized for imaging. The organ was isolated from tissue-specific transgenic lines to allow marking of a particular cell-type adjacent to the thyroid follicle. Blood vessels were marked using Tg(kdrl:EGFP) (A), macrophages using Tg(mpeg1.1:mCherry) (B) and stroma using Tg(col1a2:mCherry) (C). Thyrocytes were labeled with pax2amKO2 expression in (A) and Tg(tg:nls-EGFP) expression in (B - C). (D) NFE was labeled using antibody against TP63 in sections of the thyroid gland isolated from Tg(tg:nls-EGFP) animals. Gills are marked based on their morphological appearance. DAPI labels nuclei. Scale bars: 10 µm (A - B), 50 µm (C - D)."
NCBI gene IDs:
"EGFP: 
mCherry: 
col1a2: 336471
kdrl: 796537
mpeg1.1: 335407
tg: 567631"
Figure legend: "(A) Dot plot depicting expression entropy on Y-axis against average gene expression on X-axis for the thyrocyte population. Each dot depicts a gene, with red dots depicting genes that show statistically significant (p-value &lt; 0.05) difference in entropy from expected value. Expected value is represented by black regression line. pax2a and ctsba are marked on the graph."
NCBI gene IDs:
"ctsba: 406645
pax2a: 30425"
Figure legend: "(B) t-SNE plot of the thyrocyte cluster with expression of tg, pax2a and ctsba. The color scale represents the normalized expression counts for each gene ranging from lowest (yellow) to highest (red). (B') Histogram overlaid with density plots depicting the distribution of normalized gene expression for tg, pax2a and ctsba. "
NCBI gene IDs:
"ctsba: 406645
pax2a: 30425
tg: 567631"
Figure legend: "(B) Whole mount immunofluorescence of 9.5hpf pax2amKO2 embryos stained with anti-mKO2 antibody (red) and anti-PAX2A antibody (green). Anterior is to the left, and dorsal side is to the top. Scale bar: 0.1 mm."
NCBI gene IDs:
"mKO2: 
pax2a: 30425"
Figure legend: "(C) Whole mount immunofluorescence of 55 hpf pax2amKO2; Tg(tg:nls-EGFP) stained with PAX2A antibody (PAX2A-Ab) displays an overlap of mKO2 and PAX2A-Ab signal. The otic vesicle (OV), mid-hindbrain barrier (MHB), interneurons (IN) and thyroid gland (THY) is labelled. White dashed line represents the outline of the zebrafish larvae. Scale bar: 100 µm. Anterior to the right."
NCBI gene IDs:
"EGFP: 
mKO2: 
pax2a: 30425
tg: 567631"
Figure legend: "(D - F) Confocal microscopy imaging of a sagittal section of a 55 hpf pax2amKO2 (red); Tg(tg:nls-EGFP) (green) embryos immunostained with PAX2A antibody (cyan) showing co-localization of mKO2 and pax2a in the pronephros (D), thyroid gland (E) and mid-hindbrain barrier (F). In the thyroid gland, mKO2 (red), PAX2A-Ab (cyan) and thyrocyte-specific GFP (green) show co-localization. Scale bar: 50 µm."
NCBI gene IDs:
"EGFP: 
mKO2: 
pax2a: 30425
tg: 567631"
Figure legend: "(G , H) Snapshots from live imaging of 55 hpf pax2amKO2; Tg(tg:nls-EGFP) embryos injected with sgRNA targeting pax2a coding sequence. The anterior part of a representative control embryo (G) is shown alongside a representative crispant (H). Crispants display a strong reduction of mKO2 fluorescence, as well as an absence of GFP signal suggesting absence of thyroid (THY) tissue. White dashed line represents the outline of the zebrafish larvae. Scale bar: 100 µm. Anterior to the right."
NCBI gene IDs:
"EGFP: 
mKO2: 
pax2a: 30425
tg: 567631"
Figure legend: "(A-D) Analysis of 3 mpf thyroid gland from pax2amKO2 zebrafish shows heterogeneity in pax2a reporter expression. (A) Whole mount confocal imaging of a 3 mpf pax2amKO2 thyroid labelled with mKO2 (red), E-cadherin (cyan, not shown in 'A' for clarity reasons) and DAPI (dark blue) for nuclear localisation. (B - D) Optical sections of three follicles, with mKO2-Low cells labelled with arrows. E-cadherin (B' - D') and DAPI (B'' - D'') staining shows that absence of mKO2 signal does not correspond to an absence of cells. Anterior to the bottom of the pictures."
NCBI gene IDs:
"mKO2: 
pax2a: 30425"
Figure legend: "(E,F) (E) Confocal image of thyroid gland section from Tg(tg:nls-EGFP) at 4 mpf stained with PAX2A antibody and DAPI. The dotted region is displayed at high magnification in (F). Arrows marks thyrocytes displaying low PAX2A staining. Notably, PAX2A-Low thyrocytes display tg-driven EGFP expression, demonstrating their differentiated status."
NCBI gene IDs:
"EGFP: 
tg: 567631"
Figure legend: "(A - C) Cells from the thyroid gland of 5 mpf Tg(tg:nls-GFP); pax2amKO2 animals were labelled with calcein (live cell marker) and analysed using FACS. (A) A FACS plot showing calcein on Y-axis and GFP on X-axis. The box encompassing the GFP+ cells represents the thyrocyte population, which was gated for further analysis. (B) Histogram showing the distribution of GFP intensity in thyrocytes. (C) Histogram showing the distribution of mKO2 intensity in thyrocytes. Thyrocytes were selected by gating for GFP+ population. Horizontal lines indicate the mKO2-Low and mKO2-High expression level, with percentage values representing proportion of thyrocytes with mKO2-Low and mKO2-High expression."
NCBI gene IDs:
"mKO2: 
pax2a: 30425"
Figure legend: "(D , E) RNA-Seq. analysis of transcriptome isolated from pax2amKO2-High and pax2amKO2-Low subpopulations. (D) Volcano plot representing the differential expression of genes between the two thyrocyte subpopulations. Dots for pax2a, tg, mKO2 and EGFP are marked. Dots for genes that display significant differential expression (DESeq2, adjusted p-value &lt; 0.05) are coloured. (E) Heatmap displaying the expression of selected genes in the two subpopulations. Biological replicates are represented by individual columns."
NCBI gene IDs:
"EGFP: 
mKO2: 
pax2a: 30425
tg: 567631"
Figure legend: "293T cells were transfected with the indicated plasmid DNAs for 24 hr. The cell lysates were prepared and pulled down with Strep bead for 2 hr. Co-precipitated proteins were determined by immunoblot assay. WT, PARS1 wild type; MT, PARS1 FA/EA/RL."
NCBI gene IDs:
"PARS1: "
Figure legend: "G, H. WI-26 VA4 cells stably expressing each of EV (empty vector) or PARS1 FA/EA/RL mutant were incubated with the indicated compounds for 72 hr. Dose-response curves of the compounds for collagen levels and cytotoxicity were determined and relative ratio of CC50 to the IC50 of collagen for HF (G) and DWN12088 (H) were calculated. The IC50 values were calculated using GraphPad Prism 7.0 (collagen ELISA, n = 12 from four independent experiments (triplicate for each experiment); cytotoxicity assay, n = 8 from four independent experiments (duplicate for each experiment); Welch's t test; ***P &lt; 0.001; mean ± SEM)."
NCBI gene IDs:
"PARS1: "
Figure legend: "K. WI-26 VA4 cells stably expressing each of EV or PARS1 FA/EA/RL mutant were incubated with the indicated compounds in the presence of TGF-β for 72 hr. The levels of the indicated proteins were determined by immunoblotting. The percentage of proline in polypeptide sequences is shown on the right side of immunoblot images."
NCBI gene IDs:
"PARS1: "
Figure legend: "(A) RT-PCR analysis for temporal expression  of sinhcaf  mRNA during embryogenesis and early larval  developmental stages. NC indicates the no template control."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(B)  Time-course expression   of sinhcaf  revealed by whole-mount in situ hybridization. Scale bars, 100 μ"
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(C) RT-PCR analysis for spatial expression  of sinhcaf  mRNA in adult tissues."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(E) Relative mRNA level of sinhcaf  in  bud  stage WT and MZsinhcaf -/- mutant embryos  as measured by  RT-qPCR. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using unpaired two-tailed Student's t-test."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(G) Relative mRNA level of sinhcaf  in 10hpf or 24hpf WT and Tg(CMV:EGFP;CMV:sinhcaf) embryos  as measured by RT-qPCR. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using 2-way ANOVA followed by Holm-Sidak post-hoc test."
NCBI gene IDs:
"EGFP: 
sinhcaf: 563991"
Figure legend: "(H) Percentage of male zebrafish  in WT (n = 274), MZsinhcaf -/- (n = 166), Tg(CMV:EGFP;CMV:sinhcaf) (n = 158) and MZsinhcaf -/-;Tg(CMV:EGFP;CMV:sinhcaf) (n = 135) adult zebrafish. Data shown are mean ± SEM (n = the number of fish  analyzed). Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. The different letters (a, b, c) indicate a significant difference between the means, p&lt;0.05."
NCBI gene IDs:
"EGFP: 
sinhcaf: 563991"
Figure legend: "(A and B)  Whole-mount in situ hybridization was performed in WT, MZsinhcaf -/-, Tg(CMV:EGFP;CMV:sinhcaf) and MZsinhcaf -/-;Tg(CMV:EGFP;CMV:sinhcaf) zebrafish embryos  with PGCs  markers nanos3  (A) and vasa  (B). Scale bars, 100 μm. (C) Quantitative analysis of PGCs  in bud  stage WT, MZsinhcaf -/-, Tg(CMV:EGFP;CMV:sinhcaf) and MZsinhcaf -/-;Tg(CMV:EGFP;CMV:sinhcaf) zebrafish embryos. Data shown are mean ± SEM (n = 10, the number of embryos  analyzed). Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. The different letters (a, b, c) indicate a significant difference between the means, p&lt;0.05."
NCBI gene IDs:
"EGFP: 
vasa: 30263
nanos3: 140631
sinhcaf: 563991"
Figure legend: "(D) Maximal intensity projection of a confocal z-stack of 24hpf Tg(piwil1:EGFP) and MZsinhcaf -/-; Tg(piwil1:EGFP) zebrafish embryos, dorsal views. Arrowheads point to PGCs. Scale bars, 100 μm. (E) Quantitative analysis of PGCs  in 24hpf Tg(piwil1:EGFP) and MZsinhcaf -/-; Tg(piwil1:EGFP)zebrafish embryos. Data shown are mean ± SEM (n = 13, the number of embryos  analyzed). Statistical analysis was performed using unpaired two-tailed Student's t-test."
NCBI gene IDs:
"EGFP: 
piwil1: 368200
sinhcaf: 563991"
Figure legend: "(A) Whole-mount in situ hybridization  was performed in  4-cell stage WT, MZsinhcaf -/- zebrafish embryos  with germ plasm marker vasa. Red arrowheads point to cleavage furrows in the 4-cell embryos. Scale bars, 100 μm"
NCBI gene IDs:
"vasa: 30263
sinhcaf: 563991"
Figure legend: "(B) Relative mRNA levels of germ plasm genes vasa, dnd, nanos3, dazl  and buc  in WT and MZsinhcaf -/- mutant eggs, respectively, as measured by RT-qPCR. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using 2-way ANOVA followed by the Holm-Sidak post-hoc test; NS, no significant difference."
NCBI gene IDs:
"buc: 334375
dazl: 58039
vasa: 30263
dnd: 373074
nanos3: 140631
sinhcaf: 563991"
Figure legend: "(C) Maximal intensity projection of a confocal  z-stack of 4-cell stage WT and MZsinhcaf -/- zebrafish embryos  injected with GFP-Buc  and RFP  mRNA. White arrowheads point to  cleavage furrows of 4-cell embryos. Scale bars, 100 μm."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
sinhcaf: 563991"
Figure legend: "(D) Relative GFP-Buc  density at the cleavage furrow  of  4-cell stage  WT and MZsinhcaf -/- zebrafish embryos  injected with GFP-Buc  and RFP  mRNA. Statistical analysis was performed using unpaired two-tailed Student's t-test. Data shown are mean ± SEM (n = 12, the number of embryos  analyzed)."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
sinhcaf: 563991"
Figure legend: "(F) Western blots  for GFP-Buc  expression  in 4-cell stage WT and MZsinhcaf -/- zebrafish embryos  injected with GFP-Buc  and RFP mRNA"
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
sinhcaf: 563991"
Figure legend: "(A, B and C) Co-immunoprecipitation of zebrafish Sinhcaf-GFP  with HA-Hdac1  (A), HA-Sin3a  (B) or HA-Sap18  (C) in HEK293Tcells transfected with both Sinhcaf-GFP  and HA-Hdac1  (A), HA-Sin3a (B) or HA-Sap18  (C) plasmids."
NCBI gene IDs:
"GFP: 
HA: 
Hdac1: 192302
Sap18: 393693
Sin3a: 323346///563090
Sinhcaf: 563991"
Figure legend: "(D) Relative mRNA level of sinhcaf  in zebrafish PG, PV, EV, MV  and FG follicles  was measured using RT-qPCR. Data shown are mean ± SEM (n = 3, biological replicates). Data information: EV, early vitellogenic stage (early stage III); FG, full-grown stage  (late stage III); MV, mid vitellogenic stage (mid stage III); PG,  primary growth (stage I); PV, previtellogenic stage (stage II)."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(E and F) Western blots for acetyl-histone H3  (K9) (Ace-H3-K9),  histone H3 in WT (E) or MZsinhcaf -/- (F) PG, PV, EV, MV  and FG follicles. α-tubulin was used as a loading control. Data information: EV, early vitellogenic stage (early stage III); FG,  full-grown stage  (late stage III); MV, mid vitellogenic stage (mid stage III); PG,  primary growth (stage I); PV, previtellogenic stage (stage II)."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(G and H) Statistical analysis of the relative levels of Ace-H3-K9 in WT (G) or MZsinhcaf -/- (H) PG, PV, EV, MV  and FG follicles. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. The different letters (a, b, c) indicate a significant difference between the means, p&lt;0.05. Data information: EV, early vitellogenic stage (early stage III); FG, full-grown stage (late stage III); MV, mid vitellogenic stage (mid stage III); PG, primary growth  (stage I); PV, previtellogenic stage (stage II)."
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(I, J and K) Western blots for Ace-H3-K9 and H3  in WT and MZsinhcaf -/- EV  (I), MV  (J) and FG  (K) follicles. α-tubulin  was used as a loading control. Data information: EV, early vitellogenic stage (early stage III); FG, full-grown stage (late stage III); MV, mid vitellogenic stage (mid stage III)"
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(L) Western blots for Ace-H4-K5, Ace-H3-K18 and H3  in WT and MZsinhcaf -/- FG follicles. α-tubulin was used as a loading control. Data information: FG, full-grown stage (late stage III)"
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(M, N and O) Statistical analysis of the relative levels of Ace-H3-K9I n WT and MZsinhcaf -/- EV  (M), MV  (N) and FG  (O) follicles. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using unpaired two-tailed Student's t-test. Data information: EV, early vitellogenic stage (early stage III); FG, full-grown stage (late stage III); MV, mid vitellogenic stage (mid stage III)"
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(P and Q) Statistical analysis of the relative levels of Ace-H3-K18 (P) and Ace-H4-K5 (Q) in WT and MZsinhcaf -/- FG follicles. Data shown are mean ± SEM (n = 3, biological replicates). Statistical analysis was performed using unpaired two-tailed Student's t-test. Data information: ; FG,  full-grown stage (late stage III)"
NCBI gene IDs:
"sinhcaf: 563991"
Figure legend: "(A) Maximal intensity projection of a confocal  z-stack of 24hpf Tg(piwil:egfp), Tg(piwil:egfp) injected with kif26ab MO   and Tg(piwil:egfp) injected with control MO embryos. (B) Quantitative analysis of PGCs  in 24hpf Tg(piwil:egfp) (n = 11), Tg(piwil:egfp) injected with kif26ab MO  (n = 12) and Tg(piwil:egfp) injected with control MO  (n = 13) embryos. Data shown are mean ± SEM (n = the number of embryos  analyzed)."
NCBI gene IDs:
"egfp: 
kif26ab: 58004
piwil: 368200"
Figure legend: "(C) Maximal intensity projection of a confocal  z-stack of  4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA, GFP-Buc  mRNA + RFP mRNA + Control MO  and GFP-Buc  mRNA + RFP  mRNA + kif26ab MO. White arrowheads point to cleavage furrows of  4-cell embryos."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004"
Figure legend: "(D) Relative GFP-Buc  density at the cleavage furrow  of 4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA (n = 11), GFP-Buc  mRNA + RFP  mRNA + Control MO  (n = 11) and GFP-Buc  mRNA + RFP  mRNA + kif26ab MO  (n = 10). Data shown are mean ± SEM (n = the number of embryos analyzed)."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004"
Figure legend: "(E) Statistical analysis of the relative levels of GFP-Buc/a-Tubulin. Data shown are mean ± SEM (n = 3, biological replicates). (F)  Western blots for GFP-Buc  expression in 4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA, GFP-Buc  mRNA + RFP  mRNA + Control MO  and GFP-Buc  mRNA + RFP  mRNA + kif26ab MO. Data information: Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Scale bars, 100 μm."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004"
Figure legend: "(G) Maximal intensity projection of a confocal  z-stack of MZsinhcaf -/- or WT  4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA or GFP-Buc  mRNA + RFP  mRNA + kif26ab  mRNA. White arrowheads point to cleavage furrows  of 4-cell embryos."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004
sinhcaf: 563991"
Figure legend: "(H) Relative GFP-Buc density at the  cleavage furrow  of MZsinhcaf -/- (n = 12) or WT (n = 13) 4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA or GFP-Buc  mRNA + RFP  mRNA + kif26ab  mRNA. Data shown are mean ± SEM (n = the number of embryos  analyzed)."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004
sinhcaf: 563991"
Figure legend: "(I) Statistical analysis of the relative levels of GFP-Buc/a-Tubulin. Data shown are mean ± SEM (n = 3, biological replicates). (J) Western blots for GFP-Buc  expression in MZsinhcaf -/- or WT 4-cell stage embryos injected with GFP-Buc  mRNA + RFP  mRNA, GFP-Buc  mRNA + RFP  mRNA + Control MO  and GFP-Buc mRNA + RFP  mRNA + kif26ab MO. Data information: Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test. Scale bars, 100 μm."
NCBI gene IDs:
"GFP: 
RFP: 
Buc: 334375
kif26ab: 58004
sinhcaf: 563991"
Figure legend: "B, single optical plane in the HPuHG  of wildtype mice  and Alk1EndoKO mice  showing dramatic vascular  defect in Alk1EndoKO mice. Severely tangled and tortuous vasculatures  were outlined in white dotted rectangles."
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "C, 8 μm skin  section of Cre- control (CT) and  Alk1EndoKO mice stained  for Cdh5  (red), Ki67  (green), Erg  (white), and Dapi  (blue). Examples of Cdh5/Ki67  double positive cells were enlarged in the white dotted inset for visualization purposes."
NCBI gene IDs:
"Alk1: 11482
Cre: 2777477"
Figure legend: "D-G, Error bars represent standard deviation. Each bar represents one individual mouse, and each dot represents measurement from one image. N = 3 mice  per group, and n = 9-16 images per mouse. Restricted maximum likelihood (REML) analysis with unbounded variance components was used as statistical test and individual mouse  was treated as random effect to take into the account of mouse-to-mouse  variability. D, Quantification of number of ECs  in HPuHG  area from C. E, Percentage of proliferative ECs  (percentage of Ki67+ Cdh5+ cells in total Cdh5+ cells) in HPuHG  from C. F, Percentage of proliferative ECs  (percentage of Ki67+/Cdh5+ cells in total skin Cdh5+ cells) from C G, Percentage of proliferative ECs  (percentage of BrdU+ Cdh5+ cells in total Cdh5+ cells) in total skin  from PD17-24 BrdU-pulsed CT and Alk1EndoKO mice"
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "A-C, Immunofluorescence staining  images of 12 μm skin  section from PD17 induced PD20 telogen  CT, PD25 early anagen  CT, and PD25 telogen Alk1EndoKO mice stained  for Endomucin  (EMCN, red), CD31  (green), and Dapi  (blue). HPuHG  region was outlined in yellow dotted lines. D-F, Error bars represent standard deviation. Each bar represents one individual mouse, and each dot represents measurement from one image. N = 3 mice  per group, and n = 8-9 images per mouse. Restricted maximum likelihood (REML) analysis with unbounded variance components was used to calculate p-values and individual mouse  was treated as random effect to take into the account of mouse-to-mouse  variability. D, Quantification of EMCN+/CD31+ area from images like those in A-C, shown as percentage of BV  in the HPuHG. E, Quantification of absolute EMCN+ area (e.g. BV  area) in HPuHG  in images like those in A-C. F, Quantification of EMCN-/CD31+ area from images like those in A-C as LV  area in the HPuHG."
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "G-J, Maximum projection through optical Z-stacks of 60 μm skin  sections from mice TM-induced at PD17 and sacrificed at PD20 telogen  CT, PD25  early anagen  CT, PD32 Anagen  CT, and PD25 telogen Alk1EndoKO mice stained  for VEGFR3  (red), LYVE1  (green), and Dapi  (blue). Lymphatic capillaries  were outlined in white dotted lines. K, Quantification of lymphatic capillary caliber from images like those in G-J in PD20 telogen CT, PD25 early anagen CT, and PD25 telogen Alk1EndoKO mice  Error bars represent standard deviation. Each bar represents one individual mouse, and each dot represents measurement from one  lymphatic capillary. N = 3 mice  per group, and n = 9-25 measurements per mouse. Restricted maximum likelihood (REML) analysis with unbounded variance components was used to calculate p-values and individual mouse  was treated as random effect to take into the account of mouse-to-mouse  variability."
NCBI gene IDs:
"Alk1: 11482"
Figure legend: "F, Quantification of lymphatic capillary  caliber from images like those in D in CT and Alk1LymphKO mice  Error bars represent standard deviation. Each bar represents one individual mouse, and each dot represents measurement of one lymphatic capillary. N = 3 mice  per group, and n = 13-16 measurements per mouse. Restricted maximum likelihood (REML) analysis with unbounded variance components was used as statistical test and individual mouse  was treated as random effect to take into the account of mouse-to-mouse  variability."
NCBI gene IDs:
"Alk1: 11482"