EMBO/SourceData huggingface dataset:
Figure legend: "(C) Giemsa-stained thin films and western blot of DMSO- and RAP-treated SERA6-WT-myc3 [SERA6-mTAP:loxP] and SERA6-Cys644Ala-myc3 [SERA6-mTAP:loxP] parasites showing that cleavage of host RBC β-spectrin (red arrowhead) does not occur unless a functional copy of SERA6 is present, and is tightly associated with egress (reproducible in 2 independent experiments). Schizonts were sampled at the indicated times following washout of C2. Scale bar, 5 μm. Also see Fig EV2."
NCBI gene IDs:
"mTAP: 
myc3: 
SERA6: 812667"
Figure legend: "(A) Replication of DMSO- and RAP-treated MSA180-HA3:loxP parasites over three erythrocytic cycles. Parasitaemia values are averages from replicates in different blood sources. The similar growth rate of DMSO-treated parasites and the parental B11 clone shows that the genetic modifications to generate MSA180-HA3:loxP parasites did not affect parasite viability. Error bars, ± S.D (B11: n=3; DMSO- and RAP-treated MSA180-HA3:loxP: n=6 each)."
NCBI gene IDs:
"HA3: 
MSA180: 810296"
Figure legend: "(D) Time-course comparing fate of SERA6 from DMSO- and RAP-treated MSA180-HA3:loxP parasites (representative of 2 independent experiments). Schizonts were sampled at the indicated times following removal of C2-arrest, or arrested with E64-d (50 μM). RAP-treated SERA6-Cys644Ala-myc3 [SERA6-mTAP:loxP] parasites were included in the blot to allow comparison with processing of catalytically-dead SERA6 (SERA6-Cys644Ala-myc3). Note the accumulation of SERA6 p65 and poor conversion to SERA6 p40 in the RAP-treated (ΔMSA180) parasites."
NCBI gene IDs:
"HA3: 
mTAP: 
myc3: 
SERA6: 812667
MSA180: 810296"
Figure legend: "(A) Representative IFA images demonstrating the dynamic changes in localisation of SERA6-mTAP during egress (reproducible in 4 independent experiments). SERA6-mTAP:loxP schizonts were sampled at the indicated times following removal of C2-arrest, fixed and co-stained with DAPI (blue), anti-HA (red) and anti-ankyrin (green; ankyrin is a major component of the RBC cytoskeleton). Scale bar, 5 μm."
NCBI gene IDs:
"mTAP: 
SERA6: 812667"
Figure legend: "(C) Representative IFA images demonstrating localisation of MSA180-HA3 during egress. MSA180-HA3 [B11] schizonts were sampled at the indicated times following removal of C2-arrest, fixed and co-stained with DAPI (blue), anti-HA (red) and anti-ankyrin (green). Scale bar, 5 μm. See also Fig EV5."
NCBI gene IDs:
"HA3: 
MSA180: 810296"
Figure legend: "(C) Western blot comparing dose-response of E64-d, MMV676881 and Compound 31-mediated inhibition of maturation of SERA6-mTAP from SERA6-mTAP:loxP parasites (reproducible in 3 independent experiments)."
NCBI gene IDs:
"mTAP: 
SERA6: 812667"
Figure legend: "a, Percentage of ciliated cells in control (Ctrl) and IFT20− MEFs (*P = 0.030, n = 3) and wild-type (WT) and Ift88−/− KECs (**P = 0.003, 25 cells each, n = 3) after 24 h of serum removal. Arrows, cilia."
NCBI gene IDs:
"IFT20: 55978
Ift88: 21821"
Figure legend: "b, LC3 immunoblot (left) for autophagic flux quantification (right) in Ctrl and IFT20− MEFs (top, **P = 0.0003, n = 7), and in WT and Ift88−/− KECs (bottom, *P = 0.035, n = 9). PI, protease inhibitors; S, serum. c"
NCBI gene IDs:
"IFT20: 55978
Ift88: 21821"
Figure legend: "c, Autophagic flux in WT and Ift88−/−KECs by mCherry-GFP-LC3 puncta quantification. Puncta: yellow, autophagosomes; red only, autophagolysosomes; total, both together (**P = 0.008, *P = 0.025)."
NCBI gene IDs:
"Ift88: 21821"
Figure legend: "e, Electron microscopy images (left) and morphometric quantification of autophagic vacuoles (AV, right) in Ctrl and IFT20− MEFs (top, **P = 0.001, 10 fields in 2 experiments) and KECs WT and Ift88−/− (bottom, *P = 0.037, 5 fields). Arrows, autophagic vacuoles (AV, black) and endosomal compartments (yellow)."
NCBI gene IDs:
"IFT20: 55978
Ift88: 21821"
Figure legend: "a, GFP-LC3 puncta pattern (left) and quantification (right) in MEFs treated with purmorphamine (Purmo; top, *P = 0.028, n = 4), in Ptc−/− MEFs (middle, *P = 0.015, n = 4) and after myc-GLI1 overexpression (bottom, *P = 0.012, n = 3)."
NCBI gene IDs:
"GLI1: 14632
Ptc: 19206"
Figure legend: "c, LC3 immunoblot (left) for autophagic flux quantification (right) in Ctrl (**P = 0.001) and MEFs knocked down for SMO (SMO−) (**P = 0.0001) upon purmorphamine treatment (*P = 0.009, n = 4)."
NCBI gene IDs:
"SMO: 319757"
Figure legend: "d, Autophagic flux by mCherry-GFP-LC3 puncta quantification in WT and Ift88−/− KECs upon purmorphamine treatment (*P = 0.046, **P = 0.008, 40 fields)."
NCBI gene IDs:
"Ift88: 21821"
Figure legend: ". e, LC3 immunoblot (left) for autophagic flux quantification (right) in Ift88−/− KECs overexpressing myc-GLI1 (**P = 0.00006, n = 3)."
NCBI gene IDs:
"GLI1: 14632
Ift88: 21821"
Figure legend: "d-f, Percentage of cells with colocalizing ATGs in the basal body (BB) in WT and Ift88−/− KECs in the presence or absence of serum. d, Serum- and IFT-dependent association of ATGs with the BB (VPS34, †P = 0.04, **P = 0.002, n = 4; ATG16L, ††P = 0.0003, **P = 0.0009, 15 cells each per experiment, n = 7). e, IFT-dependent but serum-independent association of ATGs with the BB (**P = 0.001, ***P = 9.33768 × 10−6, n = 4; *P = 0.018, 15 cells each per experiment, n = 5). f, BB association of ATGs independent on serum or IFT (ATG5, n = 5; LC3, n = 8; 15 cells each per experiment). Scale bars, 10 &amp;amp;amp;mgr;m. Mean ± s.e.m."
NCBI gene IDs:
"Ift88: 21821"
Figure legend: "a, b, Immunoblot for the indicated proteins of WT and Ift88−/− KECs subjected to co-immunoprecipitation of IFT20 (a) and ATG16L (b). Inp, 1/10 input; IP, immunoprecipitate; FT, 1/10 flow-through."
NCBI gene IDs:
"Ift88: 21821"
Figure legend: "d, Immunoblot for IFT20 and ATG16L in the same fractions isolated from Ctrl, IFT20− and IFT88− MEFs in the presence (top) and absence (bottom) of serum."
NCBI gene IDs:
"IFT20: 55978
IFT88: 21821"
Figure legend: "a, b, Ciliated Atg5−/− MEFs in serum+ (*P = 0.038, 25 cells each per experiment, n = 3) (a) or serum− (*P = 0.006, nonlinear fit regression, 25 cells each per experiment, n = 3) (b)."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "e, f, GLI1 (*P = 0.04, n = 3) (e) and GLI2 (***P = 0.0005, **P = 0.0046, †P = 0.0132, n = 3) (f) messenger RNA expression in Atg5−/− MEFs."
NCBI gene IDs:
"Atg5: 11793
GLI1: 14632
GLI2: 14633"
Figure legend: "g, h, IFT20 immunoblot in Atg5−/− (g) and WT MEFs with lysosomal inhibitors (NL) (h)."
NCBI gene IDs:
"Atg5: 11793"
Figure legend: "i, IFT20 immunoblot in cytosol (Cyt), homogenate (Hom) and autophagosomes (APG). j, IFT20 protein levels in Atg5−/− MEFs (*P = 0.038, n = 6). k, IFT20 immunoblot in Atg5−/− MEFs. n.s., statistically non-significant. Mean ± s.d. in d and j and mean ± s.e.m. in other panels."
NCBI gene IDs:
"Atg5: 11793
IFT20: 55978"
Figure legend: "E) Critically short telomeres arise after 90 population doublings in the absence of telomerase (tlc1). Genomic DNA was extracted at indicated population doublings and digested with XhoI. A radioactive probe was used to recognize telomeric DNA in a southern blot. Abbreviations: PD: population doubling."
NCBI gene IDs:
"telomerase: 9164870
tlc1: 9164870"
Figure legend: "F) tlc1 cells reach senescence at PD 80-100. %viability of indicated strains after propagation in YPD is indicated at different population doublings n=3. Asterisk indicates population doubling used in G) to determine binding of Npl3 to short telomeres by TAP-ChIP. 3 independent spores from the indicated genotypes were used. Population doublings are counted from germination of meiotic products and hence begin at approx. PD40. Abbreviations: TAP: tandem affinity purification tag"
NCBI gene IDs:
"tlc1: 9164870"
Figure legend: "G) Npl3-TAP associates strongly to short telomeres. Cross-linked samples from indicated strains were used in a TAP-ChIP. Enrichment at telomeres was determined by quantitative PCR on indicated telomeres. Data represents mean % input +/- SEM n=3 (paired t-test one tailed *p&lt;0.05, **p&lt;0.01). PD90 refers to 90 population doublings in the absence of TLC1 (telomerase RNA subunit). The no tag control serves as an indicator of non-specific background signal. Abbreviations: TAP: tandem affinity purification tag, PD: population doubling."
NCBI gene IDs:
"TLC1: 9164870
telomerase: 9164870"
Figure legend: "A) Telomere interactors were identified and plotted as a volcano plot as in Figure 1 A, B and using tlc1 protein extracts. Interactors were identified using untreated protein extracts from tlc1 cells (left panel) or digested with RNase A and RNase H (right panel)."
NCBI gene IDs:
"tlc1: 9164870"
Figure legend: "B) Npl3 associates to TERRA. Cross-linked samples from the indicated strains were subjected to Npl3-TAP RIP. Enrichment of the indicated RNAs was determined by quantitative PCR on pulled-down reverse-transcribed RNA. Data represents mean % input +/- SEM n=3 (unpaired t-test two tailed *p&lt;0.05, **p&lt;0.01)."
NCBI gene IDs:
"Npl3: 852042"
Figure legend: "Npl3 associates strongly to telomeres in the absence of Rat1. C) The Rat1-AID tagged protein is strongly degraded following 1h of 1mM IAA treatment. Protein levels are determined using anti-FLAG (Rat1-AID) and anti-actin antibodies. Abbreviations: IAA: indole-3-acetic acid, AID: Auxin inducible degron."
NCBI gene IDs:
"Rat1: 854213"
Figure legend: "Npl3 associates strongly to telomeres in the absence of Rat1. D) TERRA levels increase in cells expressing the Rat1-AID variant after 1h treatment with 1mM IAA. TERRA levels were determined by quantitative PCR on reverse-transcribed RNAs after phenol-chloroform extraction. RNA levels are normalized to 7S ncRNA levels. Data represents fold increase to corresponding -IAA samples n=2. Abbreviations: IAA: indole-3-acetic acid, AID: Auxin inducible degron."
NCBI gene IDs:
"Rat1: 854213"
Figure legend: "Npl3 associates strongly to telomeres in the absence of Rat1. E) Npl3-TAP associates strongly to telomeres after 1mM IAA treatment in cells with Rat1-AID. Cross-linked samples from indicated strains were used in a TAP-ChIP. Enrichment at telomeres was determined by quantitative PCR on indicated telomeres. Data represents mean % input +/- SEM n=3 (paired t-test one tailed *p&lt;0.05, **p&lt;0.01). Abbreviations: IAA: indole-3-acetic acid, AID: Auxin inducible degron."
NCBI gene IDs:
"Rat1: 854213"
Figure legend: "F-G) Npl3 associates to telomeres in an R-loop dependent manner. E) RNH1 was overexpressed from a galactose-inducible promoter. Protein levels are determined using anti-HA and anti-actin antibodies. F) Npl3-TAP associates to telomeres in an R-loop-dependent manner. Cross-linked samples from indicated strains were used in a TAP-ChIP. Enrichment at telomeres was determined by quantitative PCR on indicated telomeres. Data represents mean % input +/- SEM n=3 (paired t-test one tailed *p&lt;0.05, **p&lt;0.01). Abbreviations: eV: empty vector control, oE: overexpression"
NCBI gene IDs:
"RNH1: 855274"
Figure legend: "C) NPL3 over expression stabilizes telomeric R-loops. The indicated strains were grown on 1% raffinose 2% galactose to induce NPL3 over expression. Cross-linked samples were subjected to DRIP. DNA-RNA hybrids were immunoprecipitated using the S9.6 antibody. R-loop levels were determined by quantitative PCR on indicated telomeres. Data represents mean % input +/- SEM relative to 250 mM HU eV n=3 (paired t-test two tailed *p&lt;0.05, **p&lt;0.01). Abbreviations eV: empty vector control, oE: over expression HU: hydroxyurea. *p&lt;0.05, **p&lt;0.01"
NCBI gene IDs:
"NPL3: 852042"
Figure legend: "D) Deletion of NPL3 decreases telomeric R-loops. The indicated strains were crosslinked and subjected to DRIP. DNA-RNA hybrids were immunoprecipitated using the S9.6 antibody. R-loop levels were determined by quantitative PCR on indicated telomeres. Data represents mean % input +/- SEM n=3. Specificity of S9.6 antibody was determined treating a fraction of the samples with RNase H."
NCBI gene IDs:
"NPL3: 852042"
Figure legend: "E-G) NPL3 stabilizes R-loops. E) R-loop dot blot. The indicated strains were grown on 1% raffinose 2% galactose for 72h and subsequently released into 1% raffinose 2% galactose or 2% glucose medium for 6 PDs. R-loop levels and dsDNA levels were determined using the S9.6 antibody and anti-dsDNA antibodies. Specificity of the S9.6 antibody was confirmed by treatment with RNase H. Loaded genomic DNA content is indicated at the bottom of the figure. F) R-loop dot blot quantification. Data represents R-loop signal as the S9.6 antibody signal relative to corresponding dsDNA signal n=3 (unpaired t-test two tailed *p&lt;0.05). G) Serial dilutions of indicated strains were assayed on 1% raffinose 2% galactose media. Plates were imaged after 72h incubation at indicated temperatures. Abbreviations: V: empty vector control, oE: over expression, ds: double-strand,"
NCBI gene IDs:
"NPL3: 852042"
Figure legend: "Quantification of the number of  quaternary branches  (indicated in schematic) in different  aman-2  alleles and in wild type control animals. All loss of function alleles of aman-2  display a significant decrease in PVD quaternary branch number. Data are represented as mean ± SEM. Statistical significance was calculated using the Kruskal-Wallis test and is indicated (****p ≤ 0.0001). n = 15 for all genotypes and are biological replicates."
NCBI gene IDs:
"aman-2: 179632"
Figure legend: "Fluorescent images and tracings of PVD  in mnr-1(dz213) hypomorphic animals alone and in combination with an aman-2(gk248486) null mutant. The dz213  introduces a L135F missense mutation in the DUF2181 domain of MNR-1/Menorin. PVD  is visualized by the dzIs53  transgene. The cell body is marked with an asterisk. Scale bars represent 20μm. Quantification of the number of full tertiaries  in genotypes indicated and traced in (F). The loss of aman-2 severely enhances the  mnr-1(dz213) phenotype. Control indicates wild type control animals as shown in (A). Data are represented as mean ± SEM. Statistical significance was calculated using the Mann-Whitney test and is indicated (****p ≤ 0.0001; ns= not significant). n &gt;23 for all genotypes and are biological replicates."
NCBI gene IDs:
"aman-2: 179632
mnr-1: 180350
Menorin: 180350
MNR-1: 180350"
Figure legend: "Fluorescent images and tracings of PVD  in kpc-1(dz254) hypomorph animals alone and in combination with an aman-2(gk248486) null mutant. PVD  is visualized by the dzIs53  transgene. White arrows indicate self-avoidance defects in tertiary branches. Red arrows show gaps between tertiary branches (no self-avoidance defects). The cell body is marked with an asterisk. Scale bars represent 20μm. Quantification of the percent of self-avoidance defects in genotypes indicated and traced in (G). Control indicates wild type control animals as shown in (A). The loss of aman-2  suppresses defects in the kpc-1(dz254) phenotype. Data are represented as mean. Statistical significance was calculated using the Z-test and is indicated (****p ≤ 0.0001). n &gt;15 for all genotypes and are biological replicates."
NCBI gene IDs:
"aman-2: 179632
kpc-1: 173051"
Figure legend: "Fluorescent images and tracings of wild type control (top) and lect-2(gk846764) hypomorphic animals (bottom) fed on plates with 300µm swainsonine  vs a DMSO  control. PVD  is visualized by the wyIs581 transgene. The  cell body  is denoted with an asterisk. Anterior is to the left and dorsal is up in all panels. Vesicular gut  autofluorescence is visible as white circular staining. Scale bars represent 20μm. Quantification of the number of full tertiaries in denoted genetic backgrounds (aman-2 null is gk248486). Black data points indicate DMSO  and orange data points show swainsonine treated animals. Data are represented as mean ± SEM. Statistical significance was calculated using the Mann-Whitney test and is indicated (****p ≤ 0.0001; ns= not significant). n = 12 for all genotypes and are biological replicates. Quantification of the number of quaternary dendrites in wild type control animals fed on plates with and without 300µm swainsonine. Animals treated with swainsonine  show a significant decrease in quaternary branch number, Data are represented as mean ± SEM. Statistical significance was calculated using the Mann-Whitney test and is indicated (****p ≤ 0.0001). n = 12 for each experiment and are biological replicates."
NCBI gene IDs:
"aman-2: 179632
lect-2: 173978"
Figure legend: "(B) Fluorescent composite images (top) and tracings (bottom) of mnr-1(dz213) in an aman-2(gk248486) and an aman-2(gk248486);  MGAT-1(null) background. An MGAT  null mutant lacks the three C. elegans paralogs: gly-12, gly-13, and gly-14. PVD  is visualized by the wyIs581 transgene. The  cell body is denoted with an asterisk. Anterior is to the left and dorsal is up in all panels. Scale bars represent 20μm. (C) Quantification of full tertiaries of denoted genotypes. Data are represented as mean ± SEM. Statistical comparisons were performed using the Kruskal-Wallis test. Statistical significance is indicated (***p ≤ 0.001, ****p ≤ 0.0001, ns=not significant). n=15 for all genotypes and are biological replicates. (D) Quantification of the number of quaternary branches in wild type control, aman-2(gk248486) null ,and MGAT1(null) animals. Loss of MGAT1  suppresses the decrease in quaternary branch  number in the aman-2(null) background. Data are represented as mean ± SEM. Statistical significance was calculated using the Kruskal-Wallis test and is indicated (**p ≤ 0.01, ****p ≤ 0.0001). n = 15 for all genotypes and are biological replicates."
NCBI gene IDs:
"aman-2: 179632
gly-12: 181005
gly-13: 181160
gly-14: 175453
mnr-1: 180350"
Figure legend: "Western blot against GFP  in C. elegans lysate expressing no transgenes (N2) and expressing DMA-1::GFP  (qyIs369), after precipitating with anti-GFP  antibody. The red boxed plus sign indicates that the lysate is treated with the PNGase F glycosidase. The downwards size shift reveals that N-glycan structures are present on DMA-1. Ladder is marked in kilodaltons (kDa). The GFP  tag contains no N-glycosylation sites. The experiment was repeated four times with biological replicates."
NCBI gene IDs:
"GFP: 
DMA-1: 187968"
Figure legend: "Western blot  against GFP  in C. elegans lysate  DMA-1::GFP  (qyIs369), after precipitating with anti-GFP  antibody. Control indicates an otherwise wild type background as opposed to an aman-2(gk248486) null background. The upward size shift in the mutant reveals that the loss of aman-2  alters the identity of N-glycan structures on DMA-1. The experiment was repeated four times with biological replicates."
NCBI gene IDs:
"aman-2: 179632"
Figure legend: "Western blot  against GFP  in C. elegans lysate DMA-1::GFP  (qyIs369), after precipitating with anti-GFP  antibody. Control indicates an otherwise wild type background as opposed to an aman-2(gk248486) null background. The red boxed +F indicates that the lysate is treated with the PNGase F glycosidase, while the green boxed +H corresponds to the Endo H glycosidase, which cleaves high-mannose  and hybrid type N-glycans. For complementary experiments using the Endo D glycosidase, which cleaves paucimannose  type N-glycans, Size shifts indicate that some hybrid/high-mannose  structures are present on DMA-1  (left), and that the  aman-2  mutant results in only hybrid/high-mannose  structures on DMA-1 (right). The experiment was repeated four times with biological replicates."
NCBI gene IDs:
"aman-2: 179632"
Figure legend: "Quantification of the number of quaternary branches in  DMA-1::2xFLAG control animals and animals with combinations of DMA-1::2xFLAG N-glycan  attachment sites mutated. Note that  DMA-1::2xFLAG control animals display a slightly reduced number of quaternary dendrites  compared to wild type animals Data are represented as mean ± SEM. Statistical significance was calculated using the Kruskal-Wallis test and Mann-Whitney between individual comparisons, and is indicated (**p ≤ 0.01, ****p ≤ 0.0001, ns=not significant). n = 15 for all genotypes and are biological replicates."
NCBI gene IDs:
"FLAG: 
DMA-1: 187968"
Figure legend: "Quantification of the number of  quaternary branches in  DMA-1::2xFLAG control animals alone and in combination with an aman-2(gk248486) null mutant (shaded in red). Control data is identical as in (B) and shown for comparison only. Data are represented as mean ± SEM. Statistical significance was calculated using the Kruskal-Wallis test and Mann-Whitney between individual comparisons, and is indicated (**p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, ns=not significant). n = 15 for all genotypes and are biological replicates."
NCBI gene IDs:
"FLAG: 
aman-2: 179632
DMA-1: 187968"
Figure legend: "Fluorescent images (left) and tracings (center) of PVD  in animals of denoted backgrounds (aman-2(null) is gk248486). S4 corresponds to the endogenous alteration of  N-glycan attachment site N386 of  DMA-1. PVD  is visualized by the dzIs117 transgene. The cell body  is marked with an asterisk. Scale bars represent 20μm."
NCBI gene IDs:
"aman-2: 179632
DMA-1: 187968"
Figure legend: "G. Representative images of astrocytes labeled with Aldh1l1-EGFP reporter in cortex and OB of Daam2 cHet and cKO mice at postnatal day 28 (P28). Scale bar: 50 μm. H-K. Overall complexity of astrocytes with Aldh1l1-EGFP reporter was measured by Sholl analysis (Two-way ANOVA) and total process length (Student's t-test). Data is presented as mean ± SEM. n= 6-10 cells from N=3-5 mice per genotype, *p&lt;0.05, p****&lt;0.0001"
NCBI gene IDs:
"Daam2: 76441"
Figure legend: "A. Representative images of GFAP immunostaining in Daam2 cKO mice compared to controls in cortex and OB at P28. Scale bar: 20 μm."
NCBI gene IDs:
"Daam2: 76441"
Figure legend: "D. Western blot analysis showed a significant increase in the amount of GFAP protein in Daam2 KO primary astrocytes. N=2"
NCBI gene IDs:
"Daam2: 76441"
Figure legend: "F. Overexpression of Daam2 via injection of AAV-GfaABC1D-myc-Daam2 in wild type P1 pups revealed reduced GFAP intensity compared to animals injected with control virus (AAV-GfaABC1D-GFP). AAV-GfaABC1D-myc-Daam2 was injected into Aldh1l1-GFP reporter mice for morphological complexity analysis and infected cells (Myc positive) were compared with non-infected cells. Dotted white line indicates virus-infected cells. Filled triangles and empty triangles indicate astrocytic primary branches with the control and reduced level of GFAP or Aldh1l1-GFP, respectively. Scale bar: 20 μm."
NCBI gene IDs:
"GFP: 
myc: 
Aldh1l1: 107747
Daam2: 76441"
Figure legend: "E. Schematics of astrocytic calcium imaging and representative images of spontaneous calcium signaling (green) of Daam2 cHet and cKO mice injected with AAV-GfaABC1D-GCaMP6f virus in OB. Approximate territory of astrocytes including soma and multiple microdomains is outlined in yellow. Representative traces of GCaMP6f signal in Daam2 cHet and cKO astrocytes are shown in black and red respectively. F-H. Quantification of amplitude (F), frequency (G), and area under curve (AUC) (H) of ∆F/F GCaMP6f signal events in OB. Amplitude and AUC of Daam2-deficient astrocytes are significantly increased in OB. Data is presented as mean ± SEM. Total number of cells is n=16, 15 (F-H) from N=3-4 mice of each genotype. Student's t-test was used for statistics, *p&lt;0.05. "
NCBI gene IDs:
"Daam2: 76441"
Figure legend: "A-F. (A, D) Schematics of whole cell recording in ex vivo brain slices and representative traces of spontaneous EPSC and IPSC in neurons in cortex from Daam2 cHet (top, black) and cKO (bottom, red) mice. (B-C, E-F) Quantification of amplitude and frequency of sEPSC and sIPSC in each genotype. Data is presented as mean ± SEM. Total number of cells is n=19, 20 (B-C) and 17, 13 (E-F) from N=7 mice for each genotype. Student's t-test was used for comparison, *p&lt;0.05."
NCBI gene IDs:
"Daam2: 76441"
Figure legend: "A. Astrocyte-specific Daam2 cKO mice show delays in buried food finding. N=13, 15 mice per genotype. Data is presented as mean ± SEM. Student's t-test was used, *p&lt;0.05."
NCBI gene IDs:
"Daam2: 76441"