EMBO/SourceData huggingface dataset:
Figure legend: "(C) Fold induction of the fluorescence of CasTA candidates using different gRNAs targeting to different locations of the GFP reporter gene compared to a strain without CasTA. The dCas9-ω was verified in the ∆rpoZ strain background, and the rest of candidates were verified in the wild-type strain (BW25113)."
NCBI gene IDs:
"GFP: 
rpoZ: 948160"
Figure legend: " (E) Different gRNAs were paired with dCas9-AsiA and dCas9-ω to profile the optimal gRNA binding distance. The dCas9-AsiA was verified in the wild-type strain (BW25113), and dCas9-ω was examined in the ∆rpoZ strain background. "
NCBI gene IDs:
"rpoZ: 948160"
Figure legend: "(A) CasTA2.1 upregulates a genomically inserted GFP reporter."
NCBI gene IDs:
"GFP: "
Figure legend: " (D) Demonstration of multiplexed CRISPRa and CRISPRi using CasTA2.1 on a reporter containing GFP and mScarlet. Parental cells had low basal GFP and high basal mScarlet expression. "
NCBI gene IDs:
"GFP: 
mScarlet: "
Figure legend: "(C, D Confocal  micrographs of HeLa  cells expressing GFP-TECPR1  and treated with osmotic shock assay or LLOMe  (C), or infected with the bacteria indicated (D) and fixed at 1 h (S. Typhimurium and L. monocytogenes) or 0.5 h (S. flexneri) post-infection. Cells were stained  for Galectin-8 and DNA (DAPI). Scale bar, 20 μm."
NCBI gene IDs:
"GFP: 
TECPR1: 25851"
Figure legend: "(F) Percentage of bacteria positive for mCherry-TECPR1 in ATG5  knockout (KO) or ATG5-complemented MEF  cells at 1 h (S. Typhimurium) or 0.5 h (S. flexneri) post-infection. Mean +/- SD of four (S. Typhimurium) or two (S. flexneri) independent experiments. n &gt; 100 bacteria per coverslip. (G) Percentage of S. Typhimurium  positive for GFP-TECPR1  at 1 h post-infection  in HeLa  cells pretreated or not with 100 nM Wortmannin. Mean +/- SD of two independent experiments. n &gt; 100 bacteria per coverslip."
NCBI gene IDs:
"ATG5: 11793"
Figure legend: "H)  Confocal  micrographs of HeLa  cells expressing GFP-TECPR1  either alone or together with FLAG-tagged neutral sphingomyelinase 2  (nSMase2; (H)) Cells were stained  for Galectin-8  and DNA (DAPI). Scale bar, 20 μm. (I) Percentage of S. Typhimurium positive for GFP-TECPR1  or endogenous Galectin-8  at 1 h post-infection  in HeLa  cells expressing FLAG-nSMase2  or not. Mean +/- SD of two (TECPR1) or three (Gal8) independent experiments. n &gt; 100 bacteria per coverslip."
NCBI gene IDs:
"FLAG: 
GFP: 
neutral sphingomyelinase 2: 55512
nSMase2: 55512
TECPR1: 25851"
Figure legend: "(E) Confocal  micrographs  of HeLa  cells expressing TECPR1  N' DysF-GFP  with or without FLAG-nSMase2, infected with S. Typhimurium, fixed at 30 min post-infection and stained  with anti-Galectin 8 antibody. DNA stained  with DAPI. Scale bar, 20 μm."
NCBI gene IDs:
"FLAG: 
GFP: 
nSMase2: 55512
TECPR1: 25851"
Figure legend: "(F) Percentage of S. Typhimurium positive for N' DysF-GFP  in Hela  cells, expressing FLAG-nSMase  or not, fixed at 30 min post-infection. Mean +/- SEM of three independent experiments performed in duplicate."
NCBI gene IDs:
"FLAG: 
nSMase: 55512"
Figure legend: "(G and H) Confocal  micrographs of HeLa  cells expressing the indicated GFP-tagged DysF  domains, infected with mCherry-S. Typhimurium and fixed at 30 min post-infection (G) or treated with osmotic shock assay, fixed and stained  with anti-Galectin 8 antibody (H). The bottom row in H depicts an enlarged version of the boxed region shown above. Scale bar, 20 μm."
NCBI gene IDs:
"GFP: 
mCherry: "
Figure legend: "(F) Confocal  micrographs of HeLa  cells expressing either GFP-TECPR1  WT or W154A and treated with LLOMe  or DMSO  vehicle control for 10 min and fixed. Scale bar, 20 μm. (G) Number of GFP-positive puncta per cell were enumerated in samples depicted in (F). Data from 50 cells from a single experiment, representative of two. p value from one-way ANOVA with Tukey's multiple comparisons test."
NCBI gene IDs:
"GFP: 
TECPR1: 25851"
Figure legend: "(A Confocal  micrographs  of indicated MEF  cell lines infected with mCherry-expressing S. Typhimurium, fixed at 30 min post-infection  and stained  with anti-ATG5  antibody (A) Scale bar, 20 μm."
NCBI gene IDs:
"mCherry: "
Figure legend: "C) Confocal  micrographs  of indicated MEF   cell lines infected with mCherry-expressing S. Typhimurium, fixed at 30 min post-infection  and stained  with anti-LC3  antibody (C). Scale bar, 20 μm."
NCBI gene IDs:
"mCherry: "
Figure legend: "(F) Confocal  micrographs of either control of ATG16L1-deficient MEF  cells, transfected with either control or TECPR1  siRNA (siRNA no. 66 depicted), infected with mCherry-expressing S. Typhimurium for 30 min, fixed and stained  with anti-LC3  antibody. Scale bar, 20 μm."
NCBI gene IDs:
"mCherry: 
ATG16L1: 77040
TECPR1: 70381"
Figure legend: "(A) Percentage of S. Typhimurium  positive for  Galectin-8 and/or GFP-TECPR1  in either control Hela  cells or those expressing GFP-TECPR1  at 1 h post-infection. Mean +/- SEM of three independent experiments performed in duplicate. n &gt; 100 bacteria per coverslip. (B) Confocal  micrographs  of HeLa  cells expressing the indicated GFP-TECPR1  constructs or not (control), infected with S. Typhimurium  for 30 min, fixed and stained  with anti-Galectin-8 and DAPI. Scale bar, 20 μm.­­ Arrowheads indicate bacteria positive for Galectin 8, of which more are present in cells expressing GFP-TECPR1  WT but not W154A. Arrow indicates bacterium to which GFP-TECPR1  but not Galectin 8 has been recruited."
NCBI gene IDs:
"GFP: 
TECPR1: 25851"
Figure legend: "(C) Percentage of S. Typhimurium  positive for  Galectin-8  in Hela  cells expressing the indicated GFP-TECPR1  constructs or not (control) and infected with S. Typhimurium  for 1 h. Mean +/- SEM of five independent experiments performed in triplicate. p value from one-way ANOVA with Tukey's multiple comparisons test."
NCBI gene IDs:
"GFP: 
TECPR1: 25851"
Figure legend: "(D) Fold intracellular proliferation  of S. Typhimurium  in HeLa  cells expressing the indicated GFP-TECPR1  constructs or not (control) as assessed by  colony forming unit  assay. Mean +/- SEM of four (ΔPH) or five (control, WT, W154A and ΔAIR) independent experiments. p value from one-way ANOVA with Dunnett's multiple comparisons test."
NCBI gene IDs:
"GFP: 
TECPR1: 25851"
Figure legend: "(B) Kaplan Meier plots of long-term overall survival of H/NSCC patients from the TCGA dataset divided on the basis of high versus low HSD17B7 expression levels relative to its median expression value among males (darkgreen and lightgreen lines) and females (red and orange lines), showing statistically significant association of elevated HSD17B7 expression with poorer patient survival. Statistical significance was calculated by the log-rank test between the groups. High_female: n=57patients. Low_female: n= 79 patients. High_male: n= 203 patients. Low_male: n= 181 patients."
NCBI gene IDs:
"HSD17B7: 51478"
Figure legend: "(D-F) Intradermal tumorigenicity assays with HKCs (269W) stably infected with an HSD17B7 overexpressing lentivirus (HSD17B7-oe) versus control (Ctrl). Experimental conditions were the same as in Fig 2. Mice were sacrificed 15 days after injection. Shown are representative images and quantification of tumor cells density assessed by immunohistochemical staining with anti-pan keratin antibodies (D,) as well as % of Ki67 positive cells (E) and K1 positivity (F) of pan-keratin positive areas. For (D), n(tumors per condition) = 9; for (E and F) n(3 fields per tumor, 5 tumors per condition) = 15. *p&lt;0.05, ****p&lt;0.0001. Horizontal line: median. 2-tail unpaired t-test. Scale bar: 500μm (D), 100μm (F)."
NCBI gene IDs:
"HSD17B7: 51478"
Figure legend: "(G, H) Intradermal tumorigenicity assays with SCC13 cells overexpressing HSD17B7 (HSD17B7-oe) versus control (Ctrl). Mice were sacrificed 15 days after injection. Shown are representative images and quantification of tumor cells density, assessed by immunohistochemical staining with anti-pan keratin antibodies (G) as well as % of Ki67 positive cells in pan-keratin positive areas (H). For (G), n(tumors per condition) = 4; for (H), n(3-5 fields per tumor, 4 tumors per condition) 15, 19. *p&lt;0.05; Horizontal line: median. One-tailed paired t-test. Scale bar in (G, H): 100μm."
NCBI gene IDs:
"HSD17B7: 51478"
Figure legend: "(D) Colony forming assays of the indicated SCC cell lines plus/minus HSD17B7 silencing as in the previous panels. Cells plated in triplicate dishes 5 days after lentiviral vector infection and selection were tested for clonogenic capability as in (A). Data are represented as number of colonies +/- SD. n(dishes per condition)= 3 *p&lt;0.05, **p&lt;0.01; p****&lt;0.0001, 2-tailed unpaired t-test."
NCBI gene IDs:
"HSD17B7: 51478"
Figure legend: "(C, D) SCC13 cells stably infected with an HSD17B7-expressing lentivirus (HSD-oe) versus empty vector control (Ctrl) (C) or two HSD17B7-silencing lentivirus (shHSD2, shHSD4) versus vector control (shCtrl) (D) were analyzed as in the previous panels. Shown are individual values for 3 parallel cultures per condition together with mean +/- SD. **p&lt;0.005; ***p&lt;0.0005; ****p&lt;0.0001. 2-tailed unpaired t-test. Additional metabolic measurement experiments of SCC cells plus/minus HSD17B7 overexpression and silencing, in the context of additional manipulations are shown in Fig 9."
NCBI gene IDs:
"HSD2: 3291
HSD17B7: 51478
HSD: 51478
HSD4: 51444"
Figure legend: "(C) Purified mitochondrial preparation from SCC13 cells infected with lentiviruses expressing wild type (HSD-wt) and Y193G and K197G mutated (HSD-mut) forms versus empty vector control (Ctrl) as in (B) were assayed for levels of Electron Transport Chain activity, ATP and mtROS production as in Fig 8A. Data are displayed as average values of triplicate measurements together with mean +/- SD. n(dishes per condition)= 3. **p&lt;0.005; ***p&lt;0.0005. 2-tailed unpaired t-test. Individual experimental values are provided in Dataset EV3."
NCBI gene IDs:
"HSD: 51478"
Figure legend: "(D) SCC13 cells were infected with two HSD17B7 silencing lentiviruses (shHSD2, shHSD4) versus vector control (shCtrl) followed, 3 days after infection and antibiotic selection, by treatment with Zymosterol (2.5uM) or ethanol vehicle alone for 48hours. Purified mitochondrial preparation were assayed for levels of Electron Transport Chain activity, ATP and mtROS production as in Fig 8A. Data are displayed as average values of triplicate measurements together with mean +/- SD. n(dishes per condition)= 3. ****p&lt;0.0001, 2-tailed unpaired t-test. Individual experimental values are provided in Dataset EV3."
NCBI gene IDs:
"HSD2: 3291
HSD17B7: 51478
HSD4: 51444"
Figure legend: "Forebrain slices from WT (A-F) and S1928A KI mice (G-L) were treated with vehicle (water) or 10 M isoproterenol (ISO) for 0.5 - 10 min before solubilization, ultracentrifugation, IP of α11.2 (A-C, G-I) or β2AR (D-F, J-L), and sequential IB for pS1928, pS1700, and α11.2, for GluA1, or for β2AR, of corresponding regions of the blots, as indicated. All the α11.2 IPs in A-C and G-I were from the same samples (which were split in half for parallel IP) as the β2AR IPs in D-F and J-L, respectively (for quantification of coIP of β2AR with α11.2 see Figure EV1F,G).(A-F) In WT, the time-dependent increase in S1928 and S1700 phosphorylation (A-C) paralleled the decrease in coIP of β2AR with α11.2 (A bottom, Figure EV1F) and of α11.2 with β2AR (D-F).(G-L) In S1928A KI mice, ISO induced S1700 phosphorylation (G,I) but did not disrupt the α11.2 - β2AR interaction (G bottom, J-L, Figure EV1G).(B,C,H,I) For quantification of α11.2 phosphorylation, pS1928 and pS1700 signals were normalized to α11.2 (**p&lt;0.01, ***p&lt;0.001, ANOVA)."
NCBI gene IDs:
"α11.2: 12288"
Figure legend: "Forebrain slices from WT (A-F) and S1928A KI mice (G-L) were treated with vehicle (water) or 10 M isoproterenol (ISO) for 0.5 - 10 min before solubilization, ultracentrifugation, IP of α11.2 (A-C, G-I) or β2AR (D-F, J-L), and sequential IB for pS1928, pS1700, and α11.2, for GluA1, or for β2AR, of corresponding regions of the blots, as indicated. All the α11.2 IPs in A-C and G-I were from the same samples (which were split in half for parallel IP) as the β2AR IPs in D-F and J-L, respectively (for quantification of coIP of β2AR with α11.2 see Figure EV1F,G).(A-F) In WT, the time-dependent increase in S1928 and S1700 phosphorylation (A-C) paralleled the decrease in coIP of β2AR with α11.2 (A bottom, Figure EV1F) and of α11.2 with β2AR (D-F).(G-L) In S1928A KI mice, ISO induced S1700 phosphorylation (G,I) but did not disrupt the α11.2 - β2AR interaction (G bottom, J-L, Figure EV1G).(B,C,H,I) For quantification of α11.2 phosphorylation, pS1928 and pS1700 signals were normalized to α11.2 (**p&lt;0.01, ***p&lt;0.001, ANOVA).(E,F,K,L) For quantification of coIP, α11.2 and GluA1 signals were normalized to β2AR (***p&lt;0.001, One Way ANOVA)."
NCBI gene IDs:
"α11.2: 12288"
Figure legend: "Graphs depict fEPSP initial slopes recorded from hippocampal CA1 before and after either a 5Hz/3 min (A-C) or 100Hz/1sec tetanus (D-F). Arrowheads mark onset of tetani and bars perfusion with 1 µM ISO. Inserts show sample traces immediately before (left) and ~30 min after (right) tetani.(A-C) Litter-matched WT but not conditional Cav1.2 KO mice showed PTT-LTP (**p &lt; 0.001, one-way ANOVA).(D-F) WT as well as Cav1.2 KO mice showed NMDAR-dependent 100 Hz LTP."
NCBI gene IDs:
"Cav1.2: 12288"
Figure legend: "(A, B) Poly(I:C) treatment inhibits axonal growth at 3 DIV (A) and dendritic growth at 6 DIV (B) in WT neurons. Neurons were transfected with GFP at 1 or 4 DIV. One day later, neurons were treated with poly(I:C) for 24 h before harvest. (C) Axon and dendrite morphology of Tlr3-/- neurons after poly(I:C) treatment. Data in A,B,C were analyzed by unpaired t-test. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. ** P &lt; 0.001, *** P &lt; 0.0001. Scale bar, 20μm in A and B."
NCBI gene IDs:
"Tlr3: 142980"
Figure legend: "(D, E) Neuronal morphology in saline- and poly(I:C)-treated Tlr3+/+;Thy1-Yfp (D) and Tlr3-/-;Thy1-Yfp (E) mouse brains. Total dendrite length, dendritic tip number and Sholl analysis of dendritic processes were used to examine the dendrite phenotype. Data of dendrite length and tip number were analyzed by unpaired t-test. The Sholl data were analyzed by two-way ANOVA with Bonferroni's multiple comparison test. The numbers of analyzed neurons and mice are presented in each column. Mean values SEM are shown. * P &lt; 0.05, ** P &lt; 0.001, *** P &lt; 0.0001. Scale bar, 50 μm in D and E."
NCBI gene IDs:
"Thy1: 21838
Tlr3: 142980"
Figure legend: "(A, B) Quantitation of dendrite morphology of Trif mutant neurons (A) and Myd88-/- neurons (B) after poly(I:C) stimulation. Trif mutant or Myd88-/- neurons were transfected with a GFP construct at 4 DIV and treated with poly(I:C) one day later. Neuron morphology was analyzed at 6 DIV according to the GFP signal. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by unpaired t-test."
NCBI gene IDs:
"Myd88: 17874
Trif: 106759"
Figure legend: "(C) MYD88 but not TRIF expression reduces dendrite outgrowth. HA-tagged MYD88 and TRIF were co-transfected with a GFP construct into cultured wild-type (WT) neurons at 4 DIV, and cell morphology was analyzed at 6 DIV. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by one-way ANOVA with Bonferroni's multiple comparison test."
NCBI gene IDs:
"MYD88: 17874
TRIF: 106759"
Figure legend: "(D) MYD88 knockdown, but not TRIF knockdown, robustly increases dendritic arbors and loses the response to poly(I:C). Three DIV neurons were transfected with control shRNA (shCtrl), MYD88 shRNA (shMYD88) or TRIF shRNA (shTRIF) and poly(I:C) was applied into the culture at 5 DIV for 24 h before harvest. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparison test. Scale bar, 20 μm. *** P &lt; 0.0001."
NCBI gene IDs:
"MYD88: 17874
TRIF: 106759"
Figure legend: "(C) Upper, schematic of MYD88 constructs. DD, death domain; ID, intermediate domain, TIR, TIR domain. Lower, coimmunoprecipitation of TLR3 and the N-terminal region of MYD88."
NCBI gene IDs:
"MYD88: 17874"
Figure legend: "(D) Full-length and N-terminal MYD88 inhibit dendrite outgrowth. Myd88-/- neurons were transfected with control vector (Ctrl), HA-tagged MYD88 (MYD88), N-terminal MYD88 (MYD88-N), or C-terminal MYD88 (MYD88-C) with a GFP construct. Poly(I:C) was applied into the culture at 5 DIV and neuronal morphology was analyzed at 6 DIV. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparison test. Scale bar, 20 μm."
NCBI gene IDs:
"MYD88: 17874
Myd88: 17874"
Figure legend: "(E) Expression patterns of full-length and truncated fragments of MYD88 in Myd88-/- neurons. Representative images are shown."
NCBI gene IDs:
"Myd88: 17874"
Figure legend: "(A) qPCR analysis of proinflammatory cytokines Il-6, Tnfα, Il-1β and antiviral cytokine Ifnβ in WT cultured cortical and hippocampal neurons. Data were analyzed by unpaired t-test. Il-6 and Tnfα N=8; Ifnβ and Il-1β, N=6."
NCBI gene IDs:
"Ifnβ: 15977
Il-1β: 16176
Il-6: 16193
Tnfα: 21926"
Figure legend: "(B, C) Poly(I:C) treatment inhibits dendrite outgrowth of Il-6-/- neurons (B) and Tnfα-/- neurons (C). Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by unpaired t-test."
NCBI gene IDs:
"Il-6: 16193
Tnfα: 21926"
Figure legend: "(D) Left, schematic of experiment using conditioned medium. Upper right, conditioned medium applied to WT neurons. Lower right, conditioned medium applied to Tlr3-/- neurons. Dendrite morphology was analyzed one day after adding conditioned medium. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by unpaired t-test. * P &lt; 0.05, *** P &lt; 0.0001."
NCBI gene IDs:
"Tlr3: 142980"
Figure legend: "(B) Expression of 9 neuropsychiatric disorder-related genes in poly(I:C)-treated Tlr3-/- neurons. Relative expression levels compared with vehicle control are shown. In (B-D), dashed lines indicate the level of saline control."
NCBI gene IDs:
"Tlr3: 142980"
Figure legend: "(C, D) Expression of Auts2, Disc1, Fmr1, Pten and Ube3a in poly(I:C)-treated Myd88-/- neurons (C) and poly(I:C)-treated P5 WT mouse brains (D). In (B-D), dashed lines indicate the level of saline control."
NCBI gene IDs:
"Auts2: 319974
Disc1: 244667
Fmr1: 14265
Myd88: 17874
Pten: 19211
Ube3a: 22215"
Figure legend: "(A) Overexpression of WT DISC1, but not the DISC1 L604F mutant, in cultured neurons suppresses dendrite withdrawal induced by TLR3 activation. Mean values SEM of representatives of three independent experiments are shown. The numbers of analyzed neurons in the representative experiments are indicated in each column. Data were analyzed by two-way ANOVA with Bonferroni's multiple comparison test. Scale bar, 20 μm. * P &lt; 0.05, ** P &lt; 0.001, *** P &lt; 0.0001."
NCBI gene IDs:
"DISC1: 244667"
Figure legend: "(B-C) Overexpression of DISC1 in cortical neurons of mouse brain is resistant to poly(I:C)-triggered reduction of dendritic arborization. Vector control (Ctrl) (B) or Myc-DISC1 (C) was co-expressed with GFP in cortical layer 2/3 neurons in mouse brain. After poly(I:C) injection at P4 and P5, the neuronal morphology was analyzed at P7 by tracing the GFP signals. The data of dendrite length and tip number were analyzed by unpaired t-test. The Sholl data were analyzed by two-way ANOVA with Bonferroni's multiple comparison test. The numbers of analyzed neurons collected from 3-4 mice of each group are indicated. Mean values SEM are shown. Scale bar, 30 μm. * P &lt; 0.05, *** P &lt; 0.0001."
NCBI gene IDs:
"DISC1: 244667"
Figure legend: "(C, D) Spine morphology of P21 Thy1-Yfp (C) and Tlr3-/-; Thy1-Yfp mice (D) after poly(I:C) stimulation at P4 and P5. One secondary dendrite of an apical dendrite of each somatosensory layer 5 cortical neuron was selected to examine the spine morphology. Three mice were analyzed for each group. Saline-treated Thy1-Yfp, 44 neurons; poly(I:C)-treated Thy1-Yfp, 43 neurons; saline-treated Tlr3-/-;Thy1-Yfp, 46 neurons; poly(I:C)-treated Tlr3-/-;Thy1-Yfp, 50 neurons. Mean values SEM are shown. Data were analyzed by unpaired t-test. Scale bar, 5 μm. *** P &lt; 0.0001."
NCBI gene IDs:
"Thy1: 21838
Tlr3: 142980"
Figure legend: " A Representative traces (left), summary graphs of normalized frequency (middle) and normalized amplitude (right) of mIPSCs recorded from cultured cortical neurons that were infected with lentivirus expressing Munc18-1 WT or mutants, with or without Munc18-1 shRNAs (Control, n = 25; None, n = 17; WT, n = 17; QKAA, n = 18; KKEE, n = 16; P335A, n = 23; L348R, n = 21). B Sample traces (left), summary graphs of normalized amplitude (middle) and normalized charge transfer (right) of evoked IPSCs recorded from neurons as described in panel A (Control, n = 19; None, n = 19; WT, n = 19; QKAA, n = 13; KKEE, n = 18; P335A, n = 17; L348R, n = 21). C Sample traces (left) and quantification of the normalized charge transfer evoked by hypertonic sucrose (right) recorded from the neurons described in panel A (Control, n = 17; None, n = 13; WT, n = 17; QKAA, n = 17; KKEE, n = 18; P335A, n = 15; L348R, n = 14). "
NCBI gene IDs:
"Munc18-1: 25558
Munc18-1: 20910"
Figure legend: " A Representative images of HEK293T cells transfected with mCherry2-Syx1 or EGFP-Munc18-1. Lipophilic dye DiD (1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine) was used as the plasma membrane marker. B Representative images of HEK293T cells co-transfected with mCherry2-Syx1 and EGFP labeled WT or mutants of Munc18-1. "
NCBI gene IDs:
"EGFP: 
mCherry2: 
Syx1: 116470
Munc18-1: 25558"
Figure legend: " C,D Summary graphs of the membrane distribution of Syx1 (C) and Munc18-1 WT or mutants (D) quantified by the ratio of fluorescence at the plasma membrane (PM) to the total fluorescence in the whole cell (total). DiD is the PM marker. Numbers of pictures analyzed are shown as follows: no Syx1, n = 14; no M18, n = 15; WT, n = 24; QKAA, n = 23; KKEE, n = 12; L348R, n = 22. "
NCBI gene IDs:
"Syx1: 116470
Munc18-1: 25558"
Figure legend: "CRTH2 subcellular localization in GFP-fused CRTH2 expressing plasmid-transfected cells. Red arrows indicate CRTH2 localized in the ER and white arrows indicate CRTH2 localized in the plasma membrane. Green, GFP-CRTH2; blue, DAPI; red, Calnexin; Scale bar, 20 μm."
NCBI gene IDs:
"GFP: 
CRTH2: 14764"
Figure legend: "Western blot analysis of CRTH2 expression in cell membrane and ER in NIH 3T3 and HEK293T cells. Myc-tagged CRTH2 expressing plasmid was transfected into NIH 3T3 and HEK293T cells. Whole cell lysate, plasma, and ER fractions were obtained 48 h after transfection. Calnexin and ATP1B were used as markers for ER and cell membrane, respectively."
NCBI gene IDs:
"Myc: 
CRTH2: 14764"
Figure legend: "Co-immunoprecipitation analysis of association of CRTH2 with LARP6. HEK293T cells were co-transfected with Myc-CRTH2 and Flag-LARP6 expressing plasmids. Whole cell lysates from HEK293T cells were immunoprecipitated and then immunoblotted with the indicated antibodies."
NCBI gene IDs:
"Flag: 
Myc: 
LARP6: 67557
CRTH2: 14764"
Figure legend: "Co-IP analysis of association of CRTH2 with GPR78. HEK293T cells were co-transfected with Myc-GPR78 expressing plasmids."
NCBI gene IDs:
"Myc: 
GPR78: 14828"