EMBO/SourceData huggingface dataset:
Figure legend: "G) Frequency of EdU positive cells with over 6 53BP1 foci in Mybl2+/+, Mybl2Δ/Δ untreated and Mybl2+/+ and Mybl2Δ/Δ plus CPT. Error bars represent SEM. Statistical analysis using unpaired two-tailed t-test. At least 200 EdU positive nuclei were analysed from 4 experimental repeats."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "A) Cells were treated for 1.5 hours with 5µM CPT, before sequential addition of IdU and CldU for 20 minutes each. Frequency of new firing origins (relative to total structures counted) from Mybl2+/+ and Mybl2Δ/Δ ESCs treated or not with CPT (n=3 independent experiments; Error bars indicate SEM). Statistical analysis using two- tailed unpaired t-test."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "(B) Distribution of replication rate in Mybl2+/+ and Mybl2Δ/Δ ESC treated or not with CPT as above. Statistical analysis was carried out using unpaired Mann Whitney U tests. A minimum of 200 forks were quantified for each of the genotypes and conditions shown from 3 independent repeats (*p &lt; 0.05; **p&lt; 0.01; ***p&lt; 0.001; **** p&lt; 0.0001)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "C) ESCs were treated with or without CPT for 4 hours before 0.1ug/ml colcemid was added during the final 3.5 hours of treatment. Representative image of H3-pS10 positive cells (turquoise). (Scale bar 10μm). Frequency of H3-pS10 positive cells in Mybl2+/+ and Mybl2Δ/Δ ESCs. Data from three independent experiments. Error bars represent SEM. Statistical analysis was carried out using a two-tailed unpaired t-test (*p &lt; 0.05; **p&lt; 0.01; ns= no significant)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "D) P-CDK1 (Tyr15), CDK1 and Beta-actin expression levels of Mybl2+/+ and Mybl2Δ/Δ ESCs with or without CPT treatment analyzed by immunoblotting. Bar graph represents average band density of P-CDK1 in Mybl2Δ/Δ ESCs relative to loading control and relative to the MYBL2+/+ from 6 repeats. Error bars represent SEM. Statistical analysis was carried out using a two-tailed unpaired t-test (**p&lt; 0.01)."
NCBI gene IDs:
"Mybl2: 17865
MYBL2: 17865"
Figure legend: "A) Immunoblot showing the levels of phosphorylated CHK1 (Ser345) and GAPDH in the Mybl2+/+ and Mybl2Δ/Δ ESCs with or without CPT treatment (2.5µM CPT for 4 hours)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "B) Immunoblot showing the levels of P-CHK1 (Ser345) and CHK1 in irradiated Mybl2+/+, and Mybl2Δ/Δ ESCs with or without 3 hours inhibitor treatment: ATR (AZ20, 5µM) and ATM (Ku60019, 10µM). Cells were also exposed to 5Gy irradiation before the final hour of inhibitor treatment. Bar graph (lower panel) represents average band density of P-CHK1 in Mybl2Δ/Δ ESCs relative to CHK1 and relative to untreated cells. Mybl2+/+ from two independent repeats and Mybl2Δ/Δ ESCs from three independent repeats. Error bars represent SD. Statistical analysis was carried out using a two-tailed unpaired t-test (*p&lt; 0.05)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "C) Cells were treated for 1.5 hours with 5µM AZ20, before sequential addition of IdU and CldU for 20 minutes each. Dot plot representing the effect of ATR inhibition upon replication rate in Mybl2+/+ and Mybl2Δ/Δ ESC. Statistical analysis of distributions was carried out using unpaired Mann Whitney U tests. A minimum of 240 forks were quantified for each of the genotypes and conditions shown from 2 independent repeats (**** p&lt; 0.0001)"
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "D) Frequency of new firing origins (relative to total structures counted) from Mybl2+/+ and Mybl2Δ/Δ ESCs treated or not with ATR inhibitor AZ20. At least 500 replication structures were counted per treatment from two independent repeats. Statistical analysis using two- tailed unpaired t-test.(*p&lt; 0.05; ns= no significant)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "E) Distribution and average of IdU/CldU fork ratio in Mybl2+/+ and Mybl2Δ/Δ ESCs with or without ATR inhibitor treatment. Percentage of highly unstable forks (ratio above 2) are indicated. Dotted line at 1 indicates positive ratio. Y-axis was cut to change scale and display large values. Statistical analysis was carried out using the Mann Whitney U test. At least 150 ratios were calculated per treatment group from two independent repeats (*p &lt; 0.05; **p&lt; 0.01; ***p&lt; 0.001; **** p&lt; 0.0001)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "C) Distribution of the average IdU/CldU ratio in Mybl2+/+ and Mybl2Δ/Δ ESCs with or without ATM inhibitor treatment. Percentage of highly unstable forks (ratio above 2) are indicated. Dotted line at 1 indicates positive ratio. Y-axis was cut to change scale and display large values. Statistical analysis was carried out using the Mann Whitney U. At least 260 ratios were calculated per treatment group from 4 separate experiments ( **** p&lt; 0.0001)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "D) Frequency of new fired origins for Mybl2+/+ and Mybl2Δ/Δ ESCs with or without ATM inhibitor treatment. Error bars represent SEM. Statistical analysis was carried out using unpaired t-test. At least 500 replication structures were counted per treatment group from 4 separate repeats."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "E) Frequency of anaphases positive for UFB positive cells (based on ERCC/PICH positive immunostaining) for Mybl2+/+, the Mybl2Δ/Δ and the Mybl2+/+ treated for 2 hours with ATM inhibitor (KU60019). Data represents at least 100 anaphases from each group from 2 experimental repeats. Statistical analysis was performed using an unpaired two-tailed t-test."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "F) Immunoblot showing the levels of phosphorylated ATM (Ser1987) and ATM in the Mybl2+/+ and Mybl2Δ/Δ ESCs with or without CPT treatment (10µM CPT for 2 hours). Bar graph (lower panel) represents average band density of P-ATM in Mybl2Δ/Δ ESCs treated with CPT relative to ATM and relative to wild type CPT treated cells from three independent repeats. Error bars represent SD. Statistical analysis was carried out using a two-tailed unpaired t-test."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "G) Distribution plot of replication speed in Mybl2+/+and ATM-/- ESC treated or not for 90 minutes with 10µM KU60019 (ATMi), before sequential addition of IdU and CldU for 20 minutes each. Statistical analysis was carried out using the Mann Whitney U test. At least 200 replication forks were counted from 3 separate experiments for wild type and ATM-/- and two separate experiments for ATM-/- treated with ATM inhibitor . ( **** p&lt; 0.0001)"
NCBI gene IDs:
"ATM: 11920
Mybl2: 17865"
Figure legend: "B and C) Distribution curve of replication fork rates for Mybl2+/+ and Mybl2Δ/Δ ESCs treated with mirin. Statistical analysis was performed using the Mann Whitney U test. n=3 experimental replicates. The value of the mean fork length is indicated above arrow ( **** p&lt; 0.0001)"
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "E) Frequency of new firing origins (relative to total structures counted) from Mybl2+/+ and Mybl2Δ/Δ ESCs treated or not with Mirin. At least 450 replication structures were counted per treatment from 3 independent repeats. Error bars represent SEM. Statistical analysis using two- tailed unpaired t-test."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "F) Distribution and the average IdU/CldU ratio in Mybl2+/+ and Mybl2Δ/Δ ESCs with or without MRE11 inhibitor treatment. The length of both incorporated labels for each elongating fork was measured in µm and the positive ratio was calculated. Percentage of highly unstable forks (ratio above 2) are indicated. Dotted line at 1 indicates positive ratio. Y-axis was cut to change scale and display large values. Statistical analysis was carried out using the Mann Whitney U test. At least 260 ratios were calculated per treatment group from 3 separate experiments.( **** p&lt; 0.0001)"
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "C ) Frequency of new firing origins (relative to total structures counted) from Mybl2+/+ and Mybl2Δ/Δ ESCs treated or not with the indicated inhibitors: CDC7 inhibitor (PHA-767491) and/or ATM inhibitor (Ku60019). At least 300 replication structures were counted per treatment. Error bars represent SEM. Statistical analysis using two- tailed unpaired t-test (*p &lt; 0.05)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "D ) Replication rate (kb/min) of Mybl2+/+ and Mybl2Δ/Δ ESCs treated with the indicated inhibitors. Statistical analysis was carried out using an unpaired Mann Whitney U test (**** p&lt; 0.0001). Minimum three independent experiments. At least 270 forks were scored for non-ATM inhibitor treated groups, and a minimum of 130 for ATM inhibitor treated groups."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "E ) Fork stability (average IdU/CldU ratio) of Mybl2+/+ ESC and Mybl2Δ/Δ ESCs treated with the indicated inhibitors alone or in combination. At least 240 ratios were calculated for non-ATM inhibitor treated groups, and a minimm of 130 for ATM inhibitor treated groups. Y-axis was cut to change scale and display large values. Statistical analysis was carried out using Mann Whitney U test (**** p&lt; 0.0001). Data for Mybl2+/+ ESCs treated with ATM inhibitor alone or in combination with CDC7 inhibitor, was collected from two independent experiments. For the rest of the conditions, a minimum of three independent repeats was performed."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "F) Immunoblot showing the levels of CDC7 and actin in the Mybl2+/+ and Mybl2Δ/Δ ESCs. Bar graph (lower panel) represents average band density of CDC7 in Mybl2Δ/Δ ESCs relative to actin and relative to Mybl2+/+ ESCs from three independent repeats. Error bars represent SD. Statistical analysis was carried out using a two-tailed unpaired t-test (ns=no significant)."
NCBI gene IDs:
"Mybl2: 17865"
Figure legend: "C, Survival assays against substrates of QacA showed loss of activity against monovalent cationic antibacterials (TPP: Tetraphenylphosphonium, Et: Ethidium), while still retaining partial activity against divalent cations (Dq: Dequalinium, Ch:Chlorhexidine). EV: pBAD empty vector. n = 3 for biological replicates. CFU indicates colony forming units. Image color was inverted from black to white background to enhance clarity."
NCBI gene IDs:
"QacA: 3921406"
Figure legend: "G, Colocalization of QacAWT or QacAD411N (tagged with GFP) on E. coli spheroplasts (stained with DAPI for viability) with ICabs (labelled with NHS-Rhodamine) was screened against ICab A4 (left) and ICab B7 (right). n = 10-30 spheroplasts were imaged for each sample."
NCBI gene IDs:
"GFP: "
Figure legend: "D, Survival assays of QacAΔEL7 and QacAD411N/ΔEL7 mutants in the presence of 10 μM Tetraphenylphosphonium (TPP) and 16 μM Chlorhexidine (Ch). Data from one representative biological replicate is shown here. Image colors were inverted for improved clarity."
NCBI gene IDs:
"QacA: 3921406"
Figure legend: "C Whole cell ethidium efflux assay done with E.coli JD838 cells expressing QacAWT or QacA5A. Empty vector (EV) was used as a control for the experiment. A graph of one independent replicate is shown with error bars representing S.E.M. of 6 technical replicates."
NCBI gene IDs:
"QacA: 3921406"
Figure legend: "G Survival assays of solely EL7 chimeric constructs compared against empty vector (EV) and QacAWT. One representative biological replicate is shown. Image color inverted for clarity."
NCBI gene IDs:
"QacA: 3921406"
Figure legend: "Whole cell-based ethidium efflux assay done with E.coli JD838 cells expressing QacAWT, its EL7 deletion construct (QacAΔEL7), and the chimeric mutants of LfrA and SmvA. All assays were done in at least 2 independent replicates. One representative replicate shown with data normalized to highest value in each trace. Error bars depict S.E.M. for six technical replicates."
NCBI gene IDs:
"LfrA: 66737507
QacA: 3921406
SmvA: 1253092"
Figure legend: "I Whole cell-based ethidium efflux assay done with E.coli JD838 cells expressing QacAWT, its EL7 deletion construct (QacAΔEL7), and the chimeric mutants of LfrA and SmvA. All assays were done in at least 2 independent replicates. One representative replicate shown with data normalized to highest value in each trace. Error bars depict S.E.M. for six technical replicates."
NCBI gene IDs:
"LfrA: 66737507
QacA: 3921406
SmvA: 1253092"
Figure legend: "(C) Quantitative real time PCR analysis showing head enrichment of dEsyt; the X-axis indicates the wild type tissues from which RNA was isolated and Y-axis indicates the dEsyt transcript level expression normalized to the loading control (RP49- Ribosomal protein 49), n=3 replicates (biological)."
NCBI gene IDs:
"Ribosomal protein 49: 
RP49: 
Esyt: 42929"
Figure legend: "(D) Quantitative real time PCR analysis showing dEsyt transcript level expression in head RNA samples from wild type and sOD. Y-axis indicates the dEsyt transcript level expression normalized to the loading control (RP49- Ribosomal protein 49), n=3 replicates (biological)."
NCBI gene IDs:
"Ribosomal protein 49: 
RP49: 
Esyt: 42929
sO: 35662"
Figure legend: "(H) Representative ON images showing the rescue of rhabdomere structural integrity in dEsytKO mutants on expressing the dEsyt::mCherry transgene. Genotypes of the flies are indicated on the left. Rearing conditions and the age of the flies are indicated on top."
NCBI gene IDs:
"mCherry: 
Esyt: 42929"
Figure legend: "(A) (i) Representative optical neutralization (ON) images showing rhabdomere structure of the indicated genotypes. Rearing conditions and the age of the flies are indicated on top. (*CD- Constant Dark). (ii) TEM images showing a single ommatidium from the photoreceptors of rdgB9 and rdgB9;dEsytKO mutants reared in 12 hr L/D cycle ( / ) for 2 days. Scale bar: 1µm (asterisk indicates degenerated rhabdomere)."
NCBI gene IDs:
"Esyt: 42929
rdgB: 32340"
Figure legend: "(D) Graph showing ERG amplitude of rdgB9 and rdgB9;dEsytKO normalized to body weight. Y-axis shows the ratio of ERG amplitude of individual flies to their body weight. X-axis indicates genotypes. Each data point represents an individual fly tested. Error bar represents s.e.m."
NCBI gene IDs:
"Esyt: 42929
rdgB: 32340"
Figure legend: "(A) Confocal images showing the localization of endogenous dEsyt protein in photoreceptors of one day old dark reared flies probed with antibody against dEsyt. Scale bar: 5µm. dEsytKO shows no staining when probed with dEsyt antibody."
NCBI gene IDs:
"Esyt: 42929"
Figure legend: "(B) Confocal images showing the localization of exogenously expressed dEsyt::mCherry protein expressed using the eye specific Rh1-Gal4 in one day old dark reared flies. Rh1-Gal4 is shown as a control. A single ommatidium is shown. Scale bar: 5 µm. Phalloidin marks F-actin staining and highlights rhabdomeres R1-R7."
NCBI gene IDs:
"Gal4: 855828
Rh1: 42367"
Figure legend: "(C) Western blot from the head extracts of wild type and dEsytKO probed with the antibody against RDGB. Rearing conditions, age of the flies and genotype is indicated on top of the blot. Tubulin was used as the loading control."
NCBI gene IDs:
"Esyt: 42929"
Figure legend: "(D, E) Confocal images showing the localization of RDGB in wild type and dEsytKO photoreceptors of flies which are (D) 1 day old-dark reared and (E) 6 days old- exposed to constant illumination. For (D) and (E) RDGB visualized using an antibody against the endogenous protein. Rhabdomeres are outlined using phalloidin which marks F-actin. Scale bar: 5 µm."
NCBI gene IDs:
"Esyt: 42929"
Figure legend: "(C) TEM images of a single ommatidium from norpAP24 photoreceptors of flies reared in dark (i) day 1 and (iii) day 14, scale bar: 1µm (red asterisk indicates R7 photoreceptor). Magnified image showing a single photoreceptor from the same ommatidium (ii) day1 (iv) day 14. Scale bar: 200 nm. Arrows indicate the SMC forming an MCS with the microvillar PM. (D) Quantification of the number of MCSs per photoreceptor (from figure 6C), X-axis indicates the genotype and age of the flies and Y-axis indicates the number of MCS/photoreceptor (i) n=30 photoreceptors from 3 separate flies (R1-R6) (ii) n=9 photoreceptors from 3 separate flies (R7) "
NCBI gene IDs:
"norpA: 31376"
Figure legend: "(E) TEM images of a single ommatidium from (i) wild type and (iii) dEsytKO photoreceptors of 0-1 day old flies reared in dark, scale bar: 1µm (red asterisk indicates R7 photoreceptor) (ii, iv) Magnified image showing a single photoreceptor from the ommatidium image shown on the left. Scale bar: 200 nm. Arrows indicate the SMC forming an MCS with the microvillar PM. (F) Quantification of the number of MCS per photoreceptor of wild type day 1 v/s dEsytKO day 1 old flies reared in dark, X-axis indicates the genotype and age of the flies and Y-axis indicates the number of MCS/photoreceptor (i) n=30 photoreceptors from 3 separate flies (R1-R6) (ii) n=9 photoreceptors from 3 separate flies (R7)."
NCBI gene IDs:
"Esyt: 42929"
Figure legend: "(A) TEM images of a single ommatidium from photoreceptors of (i) dEsytKO- day 1 (iii) dEsytKO- day 14 flies reared in dark, scale bar: 1 µm except for (Ai): 2 µm (red asterisk indicates R7 photoreceptor). (A-ii, iv) Magnified image showing a single photoreceptor from the ommatidium image shown on the left. Scale bar: 200 nm. Boxes represent the origin boxes for magnification. M- Microvillar plasma membrane, S- Sub microvillar cisternae, MCS- Membrane Contact Site, P- Pigment granules. (B) Quantification of the number of MCS per photoreceptor, X-axis indicates the genotype and age of the flies and Y-axis indicates the number of MCS/photoreceptor. n=9 photoreceptors from 3 separate flies for R7. "
NCBI gene IDs:
"Esyt: 42929"
Figure legend: "(C) TEM images of a single ommatidium from photoreceptors of (i) norpAP24;dEsytKO- day 1 (iii) norpAP24;dEsytKO- day 14 flies reared in dark, scale bar: 1 µm (red asterisk indicates R7 photoreceptor). (C-ii, iv) Magnified image showing a single photoreceptor from the ommatidium image shown on the left. Scale bar: 200 nm. Arrows indicate the SMC forming an MCS with the microvillar PM. (D) Quantification of the number of MCS per photoreceptor, X-axis indicates the genotype and age of the flies and Y-axis indicates the number of MCS/photoreceptor. (i) n=30 photoreceptors from 3 separate flies for R1-R6. (ii) n=9 photoreceptors from 3 separate flies for R7. "
NCBI gene IDs:
"Esyt: 42929
norpA: 31376"
Figure legend: "(E) TEM images of a single ommatidium from photoreceptors of (i) rdgB9-day1 and (iii) rdgB9;dEsytKO - day 1 flies reared in dark, scale bar: 1 µm (red asterisk indicates R7 photoreceptor). (E-ii, iv) Magnified image showing a single photoreceptor from the ommatidium image shown on the left. Scale bar: 200 nm. (F) Quantification of the number of MCS per photoreceptor for R1-R6 in 1 day old dark reared wild type (from Figure 5B i), rdgB9 and rdgB9;dEsytKO flies. n=30 photoreceptors from 3 separate flies. "
NCBI gene IDs:
"Esyt: 42929
rdgB: 32340"
Figure legend: "B. Survival curves of Bcs1l homozygotes without (GRAC) and with (GROX) alternative oxidase (AOX) expression (n=18-21/group). The median survival of GRAC mice was 210 days and of GROX mice 589 days with no gender difference Statistics: The survival data was analyzed using Log-rank (Mantel-Cox) test (p&lt;0.0001)"
NCBI gene IDs:
"Bcs1l: 66821
alternative oxidase: 108950628
AOX: 108950628"
Figure legend: "Gene expression of major transcriptional regulators of energy metabolism (PPAR-α, mitochondrial biogenesis (PGC-1α, TFAM) in heart at P150. (n=6/group) Box plot whiskers represent quartiles, maximum and minimum value (relative fold change 'FC' to WT). Statistics: one-way ANOVA followed by Tukey's test."
NCBI gene IDs:
"PPAR-α: 19013
PGC-1α: 19017
TFAM: 21780"
Figure legend: "Gene expression of major transcriptional regulators of energy metabolism HIF-1α) and stress responses (ATF4) in heart at P150. (n=6/group) Box plot whiskers represent quartiles, maximum and minimum value (relative fold change 'FC' to WT). Statistics: one-way ANOVA followed by Tukey's test."
NCBI gene IDs:
"ATF4: 11911
HIF-1α: 15251"
Figure legend: "mRNA expression of proline synthesis-related genes (n=6/group)) (H) Aldh18a1 and (I) Pycr1 at P150 box plots of mRNA expressions represent quartiles, minimum, and maximum value (relative fold change &quot;FC&amp;qu Statistics for graph one-way ANOVA followed by Tukey&quot;s"
NCBI gene IDs:
"Aldh18a1: 56454
Pycr1: 209027"
Figure legend: "B-E. Gene expression of Ca2+ channel gene (B) ryanodin receptor, RYR2, (C) sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA2 or ATP2A2), (D) phospholamban (PLN), and (E) endothelial nitric oxide synthase NOS3 (n=6/group) Box plots for mRNA expressio represent quartiles, minimum and maximum value (relative fold change &quot;FC&amp;qu Statistics: one-way ANOVA followed by Tukey&quot;s test for Significant differences between groups (p value) are indicated on graphs"
NCBI gene IDs:
"ATP2A2: 11938
Ca2+-ATPase: 11938
SERCA2: 11938
endothelial nitric oxide synthase: 18127
NOS3: 18127
phospholamban: 18821
PLN: 18821
RYR2: 20191"
Figure legend: "Expression of neuronal nitric oxide synthase (NOS1) mRNA (F Bar graph represent mean±SD Statistics: one-way ANOVA followed by Tukey&quot;s test for Significant differences between groups (p value) are indicated on graphs"
NCBI gene IDs:
"NOS1: 18125"
Figure legend: "H. Cardiac gene expression of dimethylarginine dimethylaminohydrolase 1 (DDAH1) (n=6/group) Box plots for mRNA expressio represent quartiles, minimum and maximum value (relative fold change &quot;FC&amp;qu Statistics: one-way ANOVA followed by Tukey&quot;s test for Significant differences between groups (p value) are indicated on graphs"
NCBI gene IDs:
"DDAH1: 69219
dimethylarginine dimethylaminohydrolase 1: 69219"
Figure legend: "(C) Decreased levels of free carnitine and modified  carnitine species in PKM2  KO cell lines as compared with their parental counterparts (n=4). Data information: Data are displayed as means ± SD. Statistical significance was assessed using two-tailed Student's t-test, ∗p &lt; 0.05, ∗∗p &lt; 0.01 and ∗∗∗p &lt; 0.001. n.s means no significance."
NCBI gene IDs:
"PKM2: 5315"
Figure legend: "(E) Increased acetyl-CoA  in PKM2 KO  MCF7 and MDA-MB 231cells as compared with their parental counterparts (n=4). (F) Decreased total  cholesterol in PKM2  KO MCF7  and  MDA-MB 231cells as compared with their parental counterparts (n=3). ( Data information: Data are displayed as means ± SD. Statistical significance was assessed using two-tailed Student's t-test, ∗p &lt; 0.05, ∗∗p &lt; 0.01 and ∗∗∗p &lt; 0.001. n.s means no significance."
NCBI gene IDs:
"PKM2: 5315"
Figure legend: "(G)  Western blot  analysis of TMEM33, ABCA1, SREBP1, HMGCR, SCAP, FASN, LDLR, FDFT1, I DI1, RNF5, INSIG 1 and 2, and Calnexin in  PKM2 KO MCF7 and MDA-MB-231 cells, and their respective parental cells. Arrow indicates nuclear SREBP1. P: precursor, N: nuclear."
NCBI gene IDs:
"PKM2: 5315"
Figure legend: "(A) Immunofluorescence of SREBP1 in  MCF7 parental and PKM2 KO cells by confocal imaging. Scale bar, 5μm. (B) Quantitation of nuclear SREBP1 intensity in A. n&gt;=25 cells as in (A) from three different fields. ( Data information: Data are displayed as means ± SD. Statistical significance was assessed using two-tailed Student's t-test, ∗p &lt; 0.05 and ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"PKM2: 5315"
Figure legend: "(G)  Western blot analysis of  TMEM33, RNF5,  SCAP, SREBP1, HMGCR,  LDLR, FDFT1,  IDI1, and PKM2 in wild type,  PKM2 KO, TMEM33 OE (pool), and two TMEM33OE clones (clones #1 and #8) MDA-MB-231 cells. Arrow indicates  nuclear SREBP1. N: nucleus  and P: precursor."
NCBI gene IDs:
"PKM2: 5315
TMEM33: 55161"
Figure legend: "(B) Western blot  analysis of PKM2, PKM1, NRF1,  TMEM33, RNF5 and SCAP levels in MEF  (PKM2fl/fl, Cre-ERT2) cells treated with 4-OHT in a time course  experiment."
NCBI gene IDs:
"Cre: 2777477
PKM2: 18746"
Figure legend: "(H)  RNF5 is involved in TMEM33 mediated SCAP polyubiquitination in  293T cells. 293T cells were stably transfected with shCtrl or shRNF5 constructs, then the indicated plasmids were co-transfected into shCtrl or shRNF5  expressing  293T cells for 18 hours followed by MG-132 treatment for 8 hours. FLAG  M2  beads were used for pull-down."
NCBI gene IDs:
"RNF5: 6048
TMEM33: 55161"