EMBO/SourceData huggingface dataset:
Figure legend: "Genome browser tracks showing open chromatin (DNase-Seq or ATAC-Seq) and ChIP-Seq in Th0, Th1 and Th17 cells at the Il17a locus (Carr et al., 2017, Ciofani et al., 2012, Vahedi et al., 2012). The grey box indicates a DHS which binds JUND."
NCBI gene IDs:
"Il17a: 16171"
Figure legend: "Fold change in gene expression in TB+ IL-2 cells for Jund -/- compared to Jund+/+ mice. Data from 2 replicates are shown."
NCBI gene IDs:
"Jund: 16478"
Figure legend: "Genome browser tracks showing open chromatin (DNase-Seq or ATAC-Seq) and ChIP-Seq in Th0, Th1 and Th17 cells at the Irf8 locus. The grey box indicates an IL-2 pDHS which binds JUND."
NCBI gene IDs:
"Irf8: 15900"
Figure legend: "The mRNA expression profiling on specific markers of stem cells (B) and transit amplifying cells captured on P7 incisors CLE followed by analysis using real-time RT-PCR (C). qRT- PCR results are in arbitrary values after normalization for GapDH. Statistics was performed on triplicates using one-way ANOVA followed by Bonferroni's test."
NCBI gene IDs:
"GapDH: "
Figure legend: "Tamoxifen induced IFT88 deletion strategy (top panel) and representative images of Sox2 (green) and Ki67 (red) immunostaining on CLE regions of the indicated mouse strains (bottom panels). Dotted lines, basement membrane. Note the significant reduction of both Sox2 and Ki67 immunolabeling upon deletion of IFT88."
NCBI gene IDs:
"IFT88: 21821"
Figure legend: "Representative IF staining of Prom1 using antibodies directed either its extracellular loop (clone 13A4, green) or cytoplasmic C-terminal end (Biorbyt, Orb129549, red) on transit amplifying cell regions of the WT vs. Prom1 KO mice. Samples are counterstained with DAPI (blue). Note the lack of Prom1 labeling in Prom1 KO mice."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "The mRNA profiling on shRNA-meditated Prom1 knockdown (3 different shRNAs were used, marked as NO.1, 2 and 3) in cultured CLESCs. qRT- PCR results are in arbitrary values after normalization for GapDH."
NCBI gene IDs:
"GapDH: 
Prom1: 19126"
Figure legend: "protein profiling on shRNA-meditated Prom1 knockdown (3 different shRNAs were used, marked as NO.1, 2 and 3) in cultured CLESCs. The immunoblotting was performed with anti-Prom1 C-terminal antibody. β-actin was used as loading control (F). Molecular mass markers are indicated."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Representative images (A) and quantitative analysis (B) of IF staining and 3D reconstruction on Sox2-positive region for WT vs. Prom1 KO mice. Ant: Anterior; Post: Posterior (A). Statistics was performed using Student's t-test: **, p &lt; 0.01. Data are represented as mean and standard deviation."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Representative IF double staining of Sox 2 (green) and Ki67 (red) on the and transit amplifying cell regions of WT vs. Prom1 KO mice on frontal sections. Samples are counterstained with DAPI (blue). Quantification of Ki67+ cell number in Sox2-positive region based on IF data from WT and Prom1 KO mice (C)."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Passaging capacity analysis of the CLESCs derived from P7 WT and Prom1 KO mice. Note the passages of cells derived from WT animals are still ongoing while those from Prom1 KO mice were arrested at the first passage. Arrows indicated cells were still under passaging while blunt bars represent cells could not be passaged and stopped at the indicated passage."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Clonogenicity assay for colony number (B) and size (C) after the first the passage of CLESCs derived from WT vs. Prom1 KO mice in the absence or presence of SHH (100ng/ml). Cells were seeded in triplicate on 24-well plates as 5,000 cell/per well."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Flow cytometry analysis of Ki67-Fucci CLESCs at different cell cycle stages in cells transfected with empty vector or full length Prom1. As a result, &gt;95% of the events have been included in the analysis. The accuracy of the strategy has been validated by two independent researchers for IF quantification using Fiji 1.0 software."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Size of individual cilia in stem cell and transit amplifying cell regions of WT and Prom1 KO mice (A) and their density in the respective regions (B). 3 animals were analyzed for each genotype. The number of quantified cilia is indicated (n)."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "Clonogenicity analysis of CLESCs treated with siArl13b and its siControl. Colonies were stained with crystal violet"
NCBI gene IDs:
"Arl13b: 68146"
Figure legend: "siRNA knockdown efficiency of Arl13b (E) in Ki67 FUCCI-CLESCs was evaluated real-time RT-PCR analysis. The results are in arbitrary values after normalization for GapDH"
NCBI gene IDs:
"GapDH: 
Arl13b: 68146"
Figure legend: "siRNA knockdown efficiency of Hdac6 (F) in Ki67 FUCCI-CLESCs was evaluated real-time RT-PCR analysis. The results are in arbitrary values after normalization for GapDH"
NCBI gene IDs:
"GapDH: 
Hdac6: 15185"
Figure legend: "Representative IF double staining and 3D reconstruction of the Prom1 (green) and AcTub (red) at the apical domain of polarized MDCK cells expressing Prom1 and its K138Q mutant (top panels). Black-white inserts show Prom1 signals only. Same cells were analyzed by SEM (bottom panels). Note that Prom1 is asymmetrically distributed along the primary cilium (white arrows) as observed in vivo (see Fig 2C) and mutation K138Q significantly impacts on the primary cilium length. MV, microvilli."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "IF staining and 3D reconstruction of AcTub-labeled (red) primary cilium at the apical membrane of parental MDCK cells (MDCK) and those cells transfected with Prom1 or its K138Q mutant. The images were used to quantify the primary cilium length (Appendix Figs S6B and C). Arrows indicate primary cilia with more than 4 μm in length."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "Parental MDCK cells or those transfected with Prom1 or K138Q mutant were grown for 7 dpc Cells were observed by phase-contrast microscopy (A) Box-whisker plots show the number of domes per 600 mm2 (A). Three images were analyzed per experiment (n = 4). 4X magnification (inset in panel A)."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "Parental MDCK cells or those transfected with Prom1 or K138Q mutant were grown for 7 dpc Cells were observed by SEM (B, top panels) or confocal laser scanning microscopy after DAPI staining (B, bottom panels, Individual cells within a given dome are indicated (B, top panels) and were quantified using three different planes (bottom, centre and top) of DAPI-labeled nuclei (B, bottom panels)."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "Parental MDCK cells or those transfected with Prom1 or K138Q mutant were grown for 7 dpc (A-C) or as indicated (D). confocal laser scanning microscopy after DAPI staining Domes of similar size were evaluated (C). Means and standard deviations are displayed. 15 domes per independent experiment were examined (n = 3). Cell density at a given time of culture (7, 10 and 14 days) was determined by counting DAPI-labeled nuclei per 0.1 mm2 (D, n = 4)."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "Mutation in the Hdac6-binding site of Prom1 increases the release of Prom1+ vesicles. 16 h-conditioned medium was subjected to differential centrifugation for 5 min at 300 x g (P1); 20 min at 1,200 x g (P2); 30 min at 10,000 x g (P3) and 1 h at 200,000 x g (P4). In parallel, detergent cell lysates were prepared. The entire pellets and 1/40 of cell lysates were analyzed by immunoblotting using antibodies against human Prom1, AcTub and β-actin (F). Molecular mass markers are indicated. All three proteins were detected in the vesicle fractions (P3 and P4) suggesting Prom1+ vesicles are released from primary cilia and microvilli."
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "Mutation in the Hdac6-binding site of Prom1 increases the release of Prom1+ vesicles. 16 h-conditioned medium was subjected to differential centrifugation for 5 min at 300 x g (P1); 20 min at 1,200 x g (P2); 30 min at 10,000 x g (P3) and 1 h at 200,000 x g (P4). In parallel, detergent cell lysates were prepared. The release of Prom1+ vesicles was quantified using its immunoreactivities associated with cell and vesicle fractions (G)"
NCBI gene IDs:
"Prom1: 8842"
Figure legend: "The mRNA expression of SHH effector genes (Gli1/2 and Glis1-3) on laser microdissection captured samples were evaluated by real-time RT-PCR analysis. The results are in arbitrary values after normalization for GapDH. Each sample was isolated from distinct mice (n = 3)."
NCBI gene IDs:
"GapDH: 
Gli1: 14632
Glis1: 230587"
Figure legend: "Volume of Sox2-positive region on the lower incisor CLE at P7 was evaluated in WT vs. Glis2 KO mice upon IF staining. Number of animals analyzed is indicated (n)."
NCBI gene IDs:
"Glis2: 83396"
Figure legend: "Representative IF staining of Prom1 using specific antibodies targeting either its extracellular loop (top panels, green) or cytoplasmic C-terminal end (bottom panels, red) on the stem cell and transit amplifying cell regions of lower incisor CLE at P7 WT and Glis2 KO mice. Samples are counterstained with DAPI (blue). Dotted lines, basement membrane. Arrowheads marks approximate boundary of stem cell regions."
NCBI gene IDs:
"Glis2: 83396"
Figure legend: "Representative IF double staining of Glis2 (red) and AcTub (green) as a marker of primary cilia in stem cell region of WT vs. Prom1 KO mice. Note the reduction of Glis2 expression in Prom1-deficient animals."
NCBI gene IDs:
"Prom1: 19126"
Figure legend: "PCR array analyses were performed on LCM captured P7 CLE of Prom1 and Glis2 KO vs. WT mice (n = 3). Arrows indicated genes with expression changed for more than 2 folder increase or decrease."
NCBI gene IDs:
"Glis2: 83396
Prom1: 19126"
Figure legend: "PCR array analyses were performed on LCM captured P7 CLE of Prom1 and Glis2 KO vs. WT mice (n = 3). The mRNA expression of gene significantly changed in the PCR array (in (C)) (D) was evaluated in WT, Prom1 and Glis2 KO mice by real-time RT-PCR analysis. Each sample was isolated from distinct mice (n = 3). The results are in arbitrary values after normalization for GapDH."
NCBI gene IDs:
"GapDH: 
Glis2: 83396
Prom1: 19126"
Figure legend: "PCR array analyses were performed on LCM captured P7 CLE of Prom1 and Glis2 KO vs. WT mice (n = 3). The mRNA expression of gene significantly changed in the Stat pathway members (E) was evaluated in WT, Prom1 and Glis2 KO mice by real-time RT-PCR analysis. Each sample was isolated from distinct mice (n = 3). The results are in arbitrary values after normalization for GapDH."
NCBI gene IDs:
"GapDH: 
Glis2: 83396
Prom1: 19126"
Figure legend: "Chromatin IP analysis of binding of Glis2 to Stat3 promoter (see Appendix Fig S9D) in the absence or presence of SHH at different concentration as indicated on triplicated samples, using specific anti-Glis2 antibodies and rabbit IgG as control."
NCBI gene IDs:
"Stat3: 20848"
Figure legend: "D. SIM  images of 8325-4∆sle1 and 8325-4∆ftsK S. aureus cells incubated with purified HisTag-Sle1-SP. External addition of the protein split the tetrads  observed in sle1  and ftsK  knock-out strains. Scale bars, 1μm."
NCBI gene IDs:
"sle1: 3919100
ftsK: 3920535"
Figure legend: "A. Absence of Sle1   in (from top to bottom) autolytic,  cell wall or total cellular extracts  and  spent medium of 8325-4∆sle1  and  8325-4∆ftsK  mutants, but not in the parental strain  NCTC8325-4, was confirmed by  western blot analysis using an anti-Sle1  antibody. All  western blot  experiments were performed with three biological replicates and representative images are shown."
NCBI gene IDs:
"sle1: 3919100
ftsK: 3920535"
Figure legend: "B. Quantification of Sle1  total cellular levels, detected by western blot  (representative gel on the bottom) in S. aureus mutant 8325-4∆ftsK and ClpX  inactivated mutants 8325-4 ClpXR95C and 8325-4∆ftsK ClpXR95C showing that ClpX  inactivation restores the presence of Sle1  in the ftsK  deletion mutant. Data information: Graphs show ratio between Sle1  amount in different samples versus the amount in parental strain  NCTC8325-4 indicated by dashed line. Data represented in scatter dot plot column graphs, where column height represents mean, and error bars are the standard deviation. Data from at least four biological replicates."
NCBI gene IDs:
"ClpX: 3919696
ftsK: 3920535"
Figure legend: "C. Quantification of Sle1  total cellular levels, detected by western blot (representative gel on the bottom) in FtsK  C-terminal deletion (8325-4∆ftsKc) or inactivated (8325-4FtsKK971A) mutants showing that Sle1  levels increase in the absence of FtsK  DNA translocase activity. Data information: Graphs show ratio between Sle1  amount in different samples versus the amount in parental strain NCTC8325-4 indicated by dashed line. Data represented in scatter dot plot column graphs, where column height represents mean, and error bars are the standard deviation. Data from at least four biological replicates."
NCBI gene IDs:
"FtsK: 3920535
ftsK: 3920535"
Figure legend: "A. Total protein extracts of strains NCTC8325-4, 8325-4 TF-GFP, 8325-4 MurC-GFP  and 8325-4 TF-GFP Sle1Tn (the latter lacking Sle1) were immunoprecipitated  with GFP-Trap Magnetic Particles. Western blot  analysis of the pulled-downed  fractions using antibodies against Sle1  and GFP  shows that Sle1  is pulled down with TF-GFP  but not with negative control MurC-GFP, suggesting a specific interaction  between TF  and Sle1."
NCBI gene IDs:
"GFP: 
MurC: 3920534
Sle1: 3919100
TF: 3919697"
Figure legend: "B. Total protein extracts of strains 8325-4 TF-3XFLAG,  8325-4 GFP-FtsK TF-3XFLAG, 8325-4sfgfp-ftsK  and 8325-4 TF-3XFLAG GFP-HU  (Input extracts, bottom panel) were immunoprecipitated  with DYKDDDDK Fab-Trap Agarose  that binds FLAG-tagged proteins. Western blot  analysis of the pulled-downed fractions (top panels) using antibodies against FLAG  and GFP  shows that GFP-FtsK, but not the negative control GFP-HU, is pulled down with TF-3XFLAG, suggesting an interaction between TF  and FtsK. For each assay, a representative image of three independent biological replicates is shown."
NCBI gene IDs:
"FLAG: 
GFP: 
gfp: 
HU: 3920245
ftsK: 3920535
FtsK: 3920535
TF: 3919697"
Figure legend: "A. SIM  images of cells expressing TF-GFP  labeled with membrane  dye FM5-95. TF-GFP  forms a gradient towards the septum  in the background of parental strain NCTC8325-4 (8325-4 TF-GFP, top panels). This localization  pattern is lost in cells lacking FtsK  (8325-4∆ftsK TF-GFP, middle panels), but not in 8325-4∆ftsKc TF-GFP  mutant, expressing FtsK  without its C-terminal domain (bottom panels). Scale bars, 1 µm."
NCBI gene IDs:
"GFP: 
FtsK: 3920535
ftsK: 3920535
TF: 3919697"
Figure legend: "B. Deconvolved epifluorescence images of  live cells  of parental strain  8325-4 Sle1-3XFLAG ∆spa and TF  deletion mutant 8325-4 Sle1-3XFLAG ∆spa ∆tig, producing Sle1-3XFLAG as the sole Sle1   copy in the cell. Normal localization  pattern of Sle1   (enriched at midcell, top panel) is altered in the absence of TF  (bottom panel) with the protein becoming more dispersed over the cell surface. See image S1 for more images of Sle1-3XFLAG surface localization  in the parental strain and the negative control of the  immunofluorescence assay. Cells were incubated with fluorescent D-amino acid HADA, which is incorporated into peptidoglycan, for cell wall visualization (colored blue). Cell surface Sle1-3XFLAG  was detected with Anti-FLAG  Monoclonal Antibody-Alexa Fluor 488 (colored green, projection of a 33 slices Z-stack shown). Scale bars, 1 µm."
NCBI gene IDs:
"FLAG: 
spa: 
Sle1: 3919100
TF: 3919697
tig: 3919697"
Figure legend: "D. Frequency of cells in each  cell cycle phase in the TF  knockout mutant 8325-4Δtig  and NCTC8325-4  parental strain, showing that the TF  mutant is delayed in cell cycle phase 3, taking longer to split the division septum. Data from three biological replicates of NCTC8325-4 (Ntotal=728) and 8325-4Δtig (Ntotal=699). Data represented in column graphs where column height represents mean and error bars are the standard deviation."
NCBI gene IDs:
"tig: 3919697
TF: 3919697"
Figure legend: "C. SIM  image and heat map representation of TF-GFP localization  in  8325-4 TF-GFP  cells grown in the presence or absence of  nalidixic acid, showing the loss of TF-GFP  gradient towards the septum  in most cells grown in the presence of the antibiotic. Scale bars, 1 µm. Heatmaps represent the average of TF-GFP localization  in 50 cells in phase 2 of the cell cycle. The color scale depicts the average normalized TF-GFP  fluorescence signal in each pixel, ranging between 0 and 1. The fraction of the area of each cell model where the normalized signal intensity is above 70% of the maximum signal is 0.47 and 0.61 in the absence or presence of  nalidixic acid, respectively, confirming that TF-GFP  becomes more dispersed over the cytoplasm  in the presence of the antibiotic."
NCBI gene IDs:
"GFP: 
TF: 3919697"
Figure legend: "D. Confocal time lapse microscopy images of HeLa cells stably expressing H2B-mCherry (red) and a microtubule marker (α-Tub-eGFP; green) 48 h after transfection of miR-449a mimic, or non-targeting control siRNA. Scale bar, 10 μm.E. Quantification of spindle rotation and duration from prometaphase to anaphase onset in time-lapse movies as in D. miR-449a mimic promotes spindle rotation. Duration from prometaphase to anaphase onset was defined as the time from nuclear envelope breakdown to anaphase onset. Spindle rotation during metaphase was measured as described in methods. Individual data points correspond to single cells (n ≥ 72 in all conditions). Normality was tested with Kolmogorov-Smirnov test. Variance between samples was tested using F test. Significance was tested by Welch's t test: p-value = 2.598e-06 comparing spindle rotation of negative control versus miR-449a mimic-transfected cells (all data points are compared). p-value = 0.0005621 (for cells with prometaphase-anaphase onset duration lower than 60 min."
NCBI gene IDs:
"miR-449a: 554213"
Figure legend: "A. The expression levels of endogenous miR-34/449 family members were measured by RT-qPCR in ventricular zone samples derived by laser microdissection of mouse cortices at E14. The levels of the different miR-34/449 family members and miR-7a-1, a highly expressed miRNA relevant in cortical progenitor biology, were determined. All concentrations were normalized (norm.) using miR-7a-1 concentration (n = 8 cortices, 2 different litters). Error bars indicate standard error."
NCBI gene IDs:
"miR-7a-1: 723902"
Figure legend: "B, C. Expression analysis of miR-34/449 by in situ hybridization using locked nucleic acid (LNA) probes in wild type cortices at E14. Mature miR-449, miR-34b and miR-34c are preferentially expressed in the subventricular (SVZ) and ventricular (VZ) zones of the neocortex. Scale bar, 50 μm (A), 10 μm (B)."
NCBI gene IDs:
"miR-34b: 723849
miR-34c: 723932
miR-449: 100190765///723868"
Figure legend: "B, C. Expression analysis of miR-34/449 by in situ hybridization using locked nucleic acid (LNA) probes in wild type cortices at E14. Mature miR-449, miR-34b and miR-34c are preferentially expressed in the subventricular (SVZ) and ventricular (VZ) zones of the neocortex. Scale bar, 50 μm (B), 10 μm (C)."
NCBI gene IDs:
"miR-34b: 723849
miR-34c: 723932
miR-449: 100190765///723868"
Figure legend: "D, E. Brains of adult mice (P23), and quantification of brain weight. Dots indicate individual brains; red line indicates median. Mice lacking miR-449abc and miR-34bc (DKO) or miR-449abc, miR-34bc, and miR-34a (TKO) have significantly smaller brains compared to littermate controls (Het). Significance was tested by Pairwaise t-test with Bonferroni correction; Het (n = 6 brains, 2 different litters) vs. DKO (n = 4 brains, 2 different litters) p-value = 0.00215; Het (n = 6 brains, 2 different litters) vs. TKO (n = 4 brains, 2 different litters) p-value = 0.00054. Subfigure G was statistically tested as in E. * indicates p ≤ 0.05, ** indicates p ≤ 0.01, *** indicates p ≤ 0.001."
NCBI gene IDs:
"miR-34a: 723848
miR-34b: 723849
miR-449a: 723868"
Figure legend: "A. Confocal images of coronal sections from E16 brains of miR-34/449 KO mice and littermate controls (Het), stained with anti-Satb2-antibody to label neurons of layers II-IV (green) and DAPI to label all cell nuclei (blue). Scale bars: 50 μm.B. Quantification of the number of Satb2-positive cells per 100 μm ventricular zone surface. Bars indicate mean ± SEM, as for all quantifications shown in this figure; n = 3 brains per genotype group, from two independent litters. Data was normalized (norm.) to the ventricular zone surface analyzed (100 µm) and relativized to the heterozygous control average value. Significance was tested by Welch's t test: p-value = 0.01146 (Het vs. KO) (n = 4 cortices per genotype group, 2 independent litters). All subfigures were statistically tested as in B. * indicates p ≤ 0.05, ** indicates p ≤ 0.01, *** indicates p ≤ 0.001."
NCBI gene IDs:
"miR-34: 723932///723849///723848"
Figure legend: "K. 3D reconstruction of a dividing radial glial progenitor at early anaphase. A coronal section of an E14 brain of a heterozygous control mouse was stained with anti-phospho-Vimentin antibody (red), anti-γ-tubulin antibody (green/yellow), phalloidin (magenta), and DAPI (blue) and imaged by 3D confocal microscopy. \"s\" indicates spindle axis, \"α\" indicates angle relative to ventricular surface plane, which was determined by a vector path as indicated by thin white lines located on the left side of the image. Yellow dots highlight the centrosomes of the spindle poles from the analyzed dividing cell. Scale bars: 5 μm.L. Quantification of spindle orientation in radial glial cells as in K for E14 brains of miR-34/449 KO and littermate controls (Het). Each dot represents a single dividing cell; p-value = 3.166e-05 (Het vs. KO) (n = 131 vs. 107 cells, n = 4 brains per genotype group, 2 independent litters)."
NCBI gene IDs:
"miR-34: 723932///723849///723848"
Figure legend: "A. Quantification of mRNA expression by RT-qPCR of candidate miR-34/449 targets that might regulate spindle orientation in E14 cortex samples from miR-34/449 KO mice compared with littermate controls (Het). Expression was normlaized (norm.) to Phosphoglycerate kinase (PGK) mRNA levels. Significance was tested by Welch's t test: p = 0.009756 (JAM-A (Het) vs. JAM-A (KO)), all the rest Het vs. KO comparisons p=n.s. (n = 4 cortices per genotype group, 2 independent litters). All subfigures were statistically tested as in A. * indicates p ≤ 0.05, ** indicates p ≤ 0.01, *** indicates p ≤ 0.001. Red bar indicates median."
NCBI gene IDs:
"JAM-A: 16456
miR-34: 723932///723849///723848
PGK: 18655"
Figure legend: "B. RT-qPCR quantification of JAM-A mRNA expression in HeLa cells 48 h after transfection of miR-449a mimic compared to cells transfected with non-targeting negative control siRNA. JAM-A mRNA Expression was normalized (norm.) to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA levels. p = 0.0001218 (Neg. ctrol. vs. miR-449a mimic) (n = 9 measurements from 3 independent replicates)."
NCBI gene IDs:
"JAM-A: 50848
GAPDH: 2597
miR-449a: 554213"
Figure legend: "C, D. mouse J110 cells were transfected with either miR-449a mimic or a negative control mimic. Whole cell extracts were subjected to immunoblot analysis to detect endogenous JAM-A and ACTIN. ACTIN was used to normalize JAM-A expression (norm.) as loading control. p = 0.04454 (Neg. ctrol. vs. miR-449a mimic) (n = 4 independent wells, 2 independent transfections)."
NCBI gene IDs:
"miR-449a: 723868"
Figure legend: "E, F. HeLa cells were cotransfected with either miR-449a mimic or a negative control mimic, together with JAM-A-GFP fusion protein and GFP. Whole cell extracts were subjected to immunoblot analysis to detect GFP. GFP was used to normalize (norm.) by transfection efficiency as loading control. p = 0.001176 (JAM-A WT Neg. ctrol. vs. miR-449a mimic) (n = 9 independent wells, 4 independent transfections)."
NCBI gene IDs:
"JAM-A: 50848
miR-449a: 554213"