EMBO/SourceData huggingface dataset:
Figure legend: "F EMSA following incubation of GlmY* with 1200 nM RapZ in absence or presence of 7.5 mM of the indicated metabolite. The RapZ/GlmY* complex is indicated by an arrow."
NCBI gene IDs:
"GlmY: 2847700"
Figure legend: "(B) RNA samples extracted from normal breast epithelial cell, MCF10A, and different subtypes of breast cancer cell lines as indicated were subjected to RT-qPCR analysis to examine the expression of circPVT1 (n = 3 biological replicates, ±s.e.m., ns: non-significant, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(C) RNA samples extracted from 28 pairs of ER-positive (ER+) breast tumor and adjacent normal tissues and 14 pairs of ER-negative (ER-) breast tumor and adjacent normal tissues were subjected to RT-qPCR analysis to examine the expression of circPVT1 (±s.e.m., *P &lt; 0.05, **P &lt; 0.01 by two-tailed Student's t-test)."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "MCF7 cells were infected with control shRNA (sh-CTL) or two independent shRNAs specifically targeting circPVT1 (sh-circPVT1#1 and sh-circPVT1#2) were subjected to RNA extraction and RT-qPCR analysis to examine the expression of circPVT1 (H) (n = 3 biological replicates, ± s.e.m., ***P &lt; 0. 001 by two-tailed Student's t-test)."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "MCF7 cells were infected with control shRNA (sh-CTL) or two independent shRNAs specifically targeting circPVT1 (sh-circPVT1#1 and sh-circPVT1#2) were subjected to colony formation assay (I) (n = 3 biological replicates, ± s.e.m., ***P &lt; 0. 001 by two-tailed Student's t-test). (J) Quantification of the crystal violet dye as shown in (I) (n = 3 biological replicates, ± s.e.m., ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(O) Tamoxifen-resistant MCF7 cells transfected with si-CTL, si-circPVT1#1, or si-circPVT1#2 were treated with or without tamoxifen (Tam, 5 μM, 72 h) followed by cell proliferation assay"
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(B) Total RNAs extracted from MCF7 cells were incubated with or without RNase R (10 units/μg RNA) at 37℃ for duration as indicated, followed by RT-qPCR analysis to examine the expression of PVT1 or circPVT1 (C) MCF7 cells were treated with Actinomycin D (10 g/ml) for duration as indicated, followed by RT-qPCR analysis to examine the expression of PVT1 or circPVT1"
NCBI gene IDs:
"circPVT1: 5820
PVT1: 5820"
Figure legend: "(F) MCF7 cells were subjected to cellular fractionation followed by RNA extraction and RT-qPCR analysis to quantify the amount of circRNA in both nucleus and cytosol of the cells. ACTIN and U6 snoRNA were served as purity control for cytosolic and nuclear fractions, respectively (n = 3 biological replicates, ± s.e.m.)."
NCBI gene IDs:
"U6 snoRNA: 
ACTIN: 60"
Figure legend: "(G) MCF7 cells transfected with si-CTL, si-circPVT1#1, or si-circPVT1#2 were subjected to RNA-FISH analysis using probe specifically targeting circPVT1. Red: circPVT1; Blue: DAPI."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(D, E) UCSC genome browser views of RNA-seq as described in (A) for TFF1 (D) and GREB1 (E) are shown."
NCBI gene IDs:
"GREB1: 9687
TFF1: 7031"
Figure legend: "(F) MCF7 cells transfected with si-CTL, si-circPVT1#1, or si-circPVT1#2 were treated with or without estrogen (E2, 10-7 M, 6 h) followed by RNA extraction and RT-qPCR analysis to examine the expression of genes as indicated (G) MCF7 cells infected with sh-CTL, sh-circPVT1#1, or sh-circPVT1#2 were treated with or without estrogen (E2, 10-7 M, 6 h) followed by RNA extraction and RT-qPCR analysis to examine the expression of genes as indicated"
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "MCF7 cells transfected with control siRNA (si-CTL) or siRNAs specifically targeting circPVT1 (si-circPVT1#1 and si-circPVT1#2) were treated with or without estrogen (E2, 10-7 M, 6 h) followed by immunoblotting analysis (D) to examine the expression of ERα"
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(E) Tumor samples as described in Fig 1K were subjected to RNA extraction and RT-qPCR analysis to examine the expression of ESR1 (n = 6 biological replicates, ±s.e.m., ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"ESR1: 2099"
Figure legend: "(H) Wild-type circPVT1-luc (circPVT1-luc (WT)) and its mutant form with the potential miR-181a-2-3p or miR-6715b-5p binding site mutated (circPVT1 (MT)-luc) were transfected into HEK293T cells with or without CTL, miR-181a-2-3p, or miR-6715b-5p mimic followed by luciferase activity measurement (n = 3 biological replicates, ±s.e.m., ns: non-significant, **P &lt; 0.01 by two-tailed Student's t-test)."
NCBI gene IDs:
"luc: 
miR-181a-2-3p: 406954
miR-6715b-5p: 102465427
circPVT1: 5820"
Figure legend: "MCF7 cells transfected with si-CTL or si-circPVT1 in the presence or absence of miR-181a-2-3p inhibitor were subjected to RT-qPCR analysis using antibodies as indicated (n = 3 biological replicates, ±s.e.m., **P &lt; 0.01, ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"miR-181a-2-3p: 406954
circPVT1: 5820"
Figure legend: "MCF7 cells transfected with si-CTL or si-circPVT1 in the presence or absence of miR-181a-2-3p inhibitor to examine the expression of genes as indicated and immunoblotting analysis (M) using antibodies as indicated"
NCBI gene IDs:
"miR-181a-2-3p: 406954
circPVT1: 5820"
Figure legend: "(P) MCF7 cells were infected with control shRNA (sh-CTL) or shRNAs specifically targeting circPVT1 (sh-circPVT1) in the presence or absence of miR-181a-2-3p inhibitor were subjected to colony formation assay. (Q) Quantification of the crystal violet dye as shown in (P) (n = 3 biological replicates, ± s.e.m., **P &lt; 0.01 by two-tailed Student's t-test)."
NCBI gene IDs:
"miR-181a-2-3p: 406954
circPVT1: 5820"
Figure legend: "(A) MCF7 cells infected with control shRNA (sh-CTL) or shRNAs specifically targeting MAVS (sh-MAVS) or STING (sh-MAVS) were transfected with control siRNA (si-CTL) or siRNA specifically targeting circPVT1 (si-circPVT1) followed by RT-qPCR analysis to examine the expression of genes as indicated (n = 3 biological replicates, ±s.e.m., ns: non-significant, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"STING: 
MAVS: 57506
circPVT1: 5820"
Figure legend: "MCF7 cells infected with sh-CTL, sh-MAVS, sh-MDA5, or sh-RIGI were transfected with si-CTL or si-circPVT1 followed by using antibodies as indicated and RT-qPCR analysis (C) to examine the expression of genes as indicated (n = 3 biological replicates, ±s.e.m., ns: non-significant, *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 by two-tailed Student's t-test)."
NCBI gene IDs:
"MDA5: 
MAVS: 57506
circPVT1: 5820
RIGI: 23586"
Figure legend: "HEK293T cells transfected with HA-tagged MAVS-FL, MAVS-NTD, or MAVS-CTD were subjected to RIP using anti-HA antibody followed by immunoblotting analysis(K) using antibodies as indicated"
NCBI gene IDs:
"HA: 
MAVS: 57506"
Figure legend: "(L) HEK293T cells transfected with HA-tagged MAVS and Flag-tagged RIGI in the presence or absence of circPVT1 were subjected to immunoprecipitation (IP) with anti-Flag M2 agarose followed by immunoblotting analysis using antibodies as indicated."
NCBI gene IDs:
"Flag: 
HA: 
MAVS: 57506
circPVT1: 5820
RIGI: 23586"
Figure legend: "MCF7 cells transfected with control ASO (ASO-CTL) or ASO specifically targeting circPVT1 (ASO-circPVT1) were subjected to RT-qPCR analysis to examine the expression of genes as indicated"
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "MCF7 cells transfected with control ASO (ASO-CTL) or ASO specifically targeting circPVT1 (ASO-circPVT1) were subjected immunoblotting analysis E) using antibodies as indicated"
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "(I, J) MCF7 cells were treated with ASO-CTL, ASO-circPVT1 (50 nM), or fulvestrant (ICI, 1 μM) for duration as indicated followed by cell proliferation assay (I) and colony formation (J) (n = 3 biological replicates, ± s.e.m., **p &lt; 0.01, ***p &lt; 0.001, day 4 by two-tailed Student's t-test)."
NCBI gene IDs:
"circPVT1: 5820"
Figure legend: "E. HeLa cells expressing Arf1-HA were serum starved overnight (E, top) and subsequently stimulated with EGF for 5 min (E, bottom) prior to fixation with PFA. Fixed cells were stained for Arf1 (HA; green) and GIV (red) and nuclei (DAPI; blue). Panels on the left show overlay of all 3 stains and representative RGB plots of sections through the Arf1-stained pixels. Panels on the right display the magnified 3D surface plots of the boxed regions in the left panels. Scale bar = 10 µm."
NCBI gene IDs:
"HA: 
Arf1: 375"
Figure legend: "C. Equal aliquots (~45 µg) of whole cell lysates of control (shControl; top) and GIV-GEM depleted (shGIV; bottom) HeLa cells were analyzed for GIV and tubulin (loading control) by immunoblotting (IB)."
NCBI gene IDs:
"GIV: 55704"
Figure legend: "D. Control (sh Control; top) and GIV-GEM depleted (shGIV; bottom) HeLa cells were co-transfected with Gαi1-YFP, Gβ1-CFP and Gγ2 (untagged) and live cells were analyzed by FRET imaging at steady-state, after being serum starved in 0.2% FBS overnight and then after stimulation with 50 nM EGF. Representative freeze-frame FRET images are shown. FRET image panels display intensities of acceptor emission due to efficient energy transfer in each pixel. FRET scale is shown in the inset. Golgi and PM regions of interest are indicated with arrows. Scale bar = 10 µm. See also Figure EV2A-B for free-frame images for additional time points in control HeLa cells."
NCBI gene IDs:
"CFP: 
YFP: 
GIV: 55704
Gαi1: 2770
Gβ1: 2782
Gγ2: 54331"
Figure legend: "I. Immunoblot shows bound Arf1 (active; top) and total Arf1 (input lysates; bottom) from equal aliquots of lysates of control (sh Control) and GIV-depleted (shGIV) HeLa cells. Cells were stimulated with EGF for the indicated time points prior to lysis. Bar graphs in Figure EV2E display the fold change in Arf1 activity normalized to t0 min. 'Low' and 'high' indicate exposures."
NCBI gene IDs:
"GIV: 55704"
Figure legend: "C. Left: Graph displays experimentally determined secretion of GFP-MMP9 in response to varying doses of EGF in control (shControl) and GIV-depleted (shGIV) HeLa cells (as in B), and quantified by band densitometry. Results are expressed as mean ± S.E.M; n = 3 biological replicates. p values were determined by two-sided unpaired t-test. Right: Schematic diagram of dose responses (mG* and secretion) for the single switch and coupled switches. Coupled switches stretch the range of proportionate responses. Single mG switch results in misaligned responses. DoRA, dose response alignment."
NCBI gene IDs:
"GIV: 55704"
Figure legend: "G-H. Control (parental) and GIV-depleted (GIV KO) HeLa cells grown in different concentrations of serum (FBS%) were treated or not with varying concentrations of BFA (µM) as indicated. Line graphs in 3D (G) depict the formazan absorbance expressed as a measure of cell viability from the HeLa cells in various conditions tested. Bar graphs (H) depict the cell number in serum-free growth conditions that are supported exclusively by autocrine secrete-and-sense loop (without BFA; BFA = 0.0 µM) or when such loop is interrupted (BFA = 0.1 µM). Results are expressed as mean ± S.E.M; n = 3 biological replicates. Statistical significance was determined by one way ANOVA."
NCBI gene IDs:
"GIV: 55704"
Figure legend: "I-K. Control (parental) and GIV-depleted (GIV KO) MDA MB-231 cells grown in different concentrations of serum (FBS%) were treated or not with varying concentrations of BFA (µM) as in G-H. Line graphs in 3D (I) depict the formazan absorbance expressed as a measure of cell viability from the MDA MB-231 cells in various conditions tested. Bar graphs (J) depict the viability of the MDA MB-231 cells in serum-free growth conditions that are supported exclusively by autocrine secrete-and-sense loop (without BFA; BFA = 0.0 µM) or when such loop is interrupted (BFA = 0.1 µM). Results are expressed as mean ± S.E.M; n = 3 biological replicates. Statistical significance was determined by one way ANOVA. Immunoblots (K) of equal aliquots of whole cell lysates confirm the depletion of GIV compared to tubulin (loading control). See also Appendix Figure S5D-H for dot plots and early and late apoptotic fractions. Results are expressed as mean ± S.E.M; n = 3 biological replicates."
NCBI gene IDs:
"GIV: 55704"
Figure legend: "Double immunolabeling of PDS5A (green, A-E) and SYCP3 (red) in Pds5AB cKO spread spermatocytes at the indicated stages."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of PDS5B (green, F-J) and SYCP3 (red) in Pds5AB cKO spread spermatocytes at the indicated stages."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "A-H Double immunolabeling of SYCP1 (green) and SYCP3 (red) in wild-type (A-D) and Pds5AB cKO (E-H) spread spermatocytes at the indicated stages. I-L Double immunolabeling of HORMAD2 (pseudocolored in white) and SYCP3 (red). Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm. "
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and the cohesin subunits SMC3 (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and the cohesin subunits REC8 (green in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and the cohesin subunits SMC1β (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and the cohesin cofactors Sororin (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and the cohesin cofactors WAPL (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and either γH2AX (pseudocolored in blue in Pds5AB cKO spread spermatocytes at the indicated stages."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAD51 (green in Pds5AB cKO spread spermatocytes at the indicated stages. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "J-L Double immunolabeling of MLH1 (pseudocolored in white) and SYCP3 (red) in Pds5AB cKO spread spermatocytes. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and TRF1 (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAP1 (green, in Pds5AB cKO spread spermatocytes at the indicated stages. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "I Selected zygotene-like and pachytene-like bivalents presenting altered TRF1 distribution as (from left to right) (i) telomere stretches, (ii) multiple telomere, (iii) distant telomeres and (iv) telomere-less. Arrowheads in (I) indicate the position of altered telomeres. J Quantification of telomeres with a regular or altered disposition of TRF1 in Pds5AB cKO spermatocytes at the indicated stages (n=1646 telomeres in zygotene-like spermatocytes and 738 in pachytene-like ones). Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "M-P Double immunolabeling of SUN1 (green) and SYCP3 (red) in Pds5AB cKO spread spermatocytes at the indicated stages. 0, 1 and 2 in (N and O) indicate the number of chromosome ends labelled by SUN1 in a given bivalent. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "R Selected zygotene-like and early pachytene-like bivalents from Pds5AB cKO spermatocytes immunolabeled with SYCP3 (red) and SUN1 (green), and their respective percentage of appearance. S Quantification of telomeres with a regular or altered disposition of SUN1 (n=715 telomeres in zygotene-like spermatocytes and 410 in pachytene-like ones). Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "A-C Immunolabeling of SYCP3 (red) and telomere FISH (PNA-Tel, green) in Pds5AB cKO spread spermatocytes at the indicated stages. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "D 300% magnification of selected zygotene-like and pachytene-like bivalents presenting regular (left) or altered (i-iv) telomere FISH signals. E Quantification of telomeres with a regular or altered disposition of telomeric DNA in Pds5AB cKO spermatocytes at the indicated stages (n=1198 telomeres in zygotene-like spermatocytes and 574 in pachytene-like ones)."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAP1 (blue) and telomere FISH (PNA-Tel, green) in Pds5AB cKO spread spermatocytesat the indicated stages. Asterisks indicate the position of the enlarged chromosomes/bivalents shown in a 300% magnification displaying telomere stretches, multiple telomere, distant telomeres and telomere-less. Yellow arrowheads in (I) indicate telomeres displaying a distant telomere configuration observed both by telomere FISH and RAP1 immunolabeling. White arrowheads indicate telomeres with an altered organization of telomeric DNA into multiple telomere signals without RAP1 signals Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAP1 (blue) and telomere FISH (PNA-Tel, green) in Pds5AB cKO spread spermatocytes at the indicated stages. Asterisks indicate the position of the enlarged chromosomes/bivalents shown in a 300% magnification displaying telomere stretches, multiple telomere, distant telomeres and telomere-less. White arrowheads indicate telomeres with an altered organization of telomeric DNA into multiple telomere signals with RAP1 signals and telomere stretches Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAP1 (blue) and telomere FISH (PNA-Tel, green) in Pds5AB cKO spread spermatocytes at the indicated stages. Asterisks indicate the position of the enlarged chromosomes/bivalents shown in a 300% magnification displaying telomere stretches, multiple telomere, distant telomeres and telomere-less. White arrowheads indicate telomeres with an altered organization of telomeric DNA into multiple telomere signals without RAP1 signals telomere stretches Pink arrowheads in (T) indicate a telomere presenting a regular telomere FISH signal without a RAP1 signal. Data information: Sex bivalents (XY) are indicated. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of SYCP3 (red) and RAP1 (blue) and telomere FISH (PNA-Tel, green) in Pds5AB cKO spread spermatocytes at the indicated stages. Asterisks indicate the position of the enlarged chromosomes/bivalents shown in a 300% magnification displaying telomere stretches, multiple telomere, distant telomeres and telomere-less."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of TRF1 (green) and SYCP3 (red) in squashed spermatocytes from wild-type or Pds5AB cKO mice M-P). The first three columns correspond to z-projections of 15 focal planes throughout the Top, Equator and Bottom regions of the nucleus. The projection of 65 focal planes across the same spermatocyte nucleus is shown at the fourth column (Z-Projection). White arrowheads in (F indicate the presence of TRF1 signals non-attached to the NE. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Double immunolabeling of TRF1 (green) and SYCP3 (red) in squashed spermatocytes from wild-type or Pds5AB cKO mice The first three columns correspond to z-projections of 15 focal planes throughout the Top, Equator and Bottom regions of the nucleus. The projection of 65 focal planes across the same spermatocyte nucleus is shown at the fourth column (Z-Projection). White arrowheads in N) indicate the presence of TRF1 signals non-attached to the NE. Scale bar, 10 μm."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "Q-T Selected bivalents from Pds5AB cKO spermatocytes displaying structural telomere abnormalities as telomere stretches (Q), multiple telomere (R), distant telomere (S) and telomere-less (T)."
NCBI gene IDs:
"Pds5A: 71521"
Figure legend: "(B-E) Co-localization of EGFP and the two CA isoforms with filamentous actin studied in fibroblasts expressing (B) EGFP, (C) EGFP-CA2, or (D, E) EGFP-CA7 and stained with phalloidin-594 to visualize F-actin (n = 4, 10 and 8 independent replicates, respectively). A magnification of the area marked with the yellow rectangle in (C) and (D) shows the localization of EGFP-CA2 and EGFP-CA7 compared to phalloidin-594. (E) EGFP-CA7 caused a prominent overexpression phenotype with thick and curvy cytosolic actin bundles (arrow) and plasmalemmal protrusions (arrowhead)."
NCBI gene IDs:
"CA2: 12349
CA7: 12354"
Figure legend: "(A,B) NIH3T3 cells transfected with DsRed (A) or DsRed-CA7 (B) were incubated in growth medium with 5 µM Latrunculin B for 0, 2, 5, 10, or 30 minutes, or in an equal amount of DMSO for 60 minutes. Analyses of experiments show that in cells transfected with DsRed-CA7 F-actin structures collapse more slowly (0 min: 83% \"normal\"; 2 min: 80%, p = 0.44; 5 min: 74%, p = 0.39; 10 min: 27%, p = 0.08; 30 min: 16%, p = 0.02; DMSO: 82%, p = 0.99; tested against 0 min with two-way ANOVA, Dunnett's multiple comparison test) than in the DsRed-transfected ones (0 min: 89% \"normal\"; 2 min: 48%, p = 0.14; 5 min: 31%, p = 0.04; 10 min: 2%, p = 0.001; 30 min: 0.7%, p = 0.001; DMSO: 88%, p = 0.8; tested against 0 min with two-way ANOVA, Dunnett's multiple comparison test) . For the analysis, cells were categorized to three groups as \"normal\", \"some shape/F-actin left\" and \"round\". The upper panel shows example images of the cells in all three categories for (A) DsRed- or (B) DsRed-CA7 transfected cells (actin visualized with Phalloidin-488)."
NCBI gene IDs:
"DsRed: 
CA7: 12354"
Figure legend: "(E) Pearson's correlation coefficient values calculated for the depicted constructs and compared to CA7. F-actin had a strong positive correlation coefficient with EGFP-CA7 (r = 0.91 ± 0.02, n = 26 cells). Neither EGFP alone (r = 0.01 ± 0.03, n = 56) nor EGFP-CA2 (r = 0.09 ± 0.03, n = 53) co-localized with F-actin (P &lt; 0.0001 for both constructs, when compared to CA7). From the five mutated CA7 constructs, EGFP-CA7-mutant1 (r= 0.51 ± 0.04, P &lt; 0.0001, n = 25) and EGFP-CA7-mutant3 (r = 0.72 ± 0.03, P = 0.05, n = 21) co-localized less with F-actin actin when compared to CA7. The co-localization of the other four mutated CA7 constructs, EGFP-CA7-mutant2 (r = 0.92 ± 0.02, P = 0.99, n = 18), EGFP-CA7-R223E (r = 0.86 ± 0.02, P = 0.99, n = 24) and EGFP-CA7-H96/98C (r = 0.79 ± 0.04, P = 0.14, n = 18) did not differ significantly from that of CA7. Data are shown as mean ± SEM. Data did not pass Shapiro-Wilk test for normality, statistical comparison against CA7 was done with Kruskall-Wallis test corrected for multiple comparisons."
NCBI gene IDs:
"EGFP: 
CA2: 12349
CA7: 12354"