EMBO/SourceData huggingface dataset:
Figure legend: "(C) RLUC-ATG8a  or RLUC-NBR1  constructs were coexpressed with internal control FLUC  in N. benthamiana. Xcv ΔxopQ was co-infiltrated with Agrobacteria containing the luciferase  reporter constructs. Coexpression of RFP-AIMp   serves as a control for autophagy  inhibition. Expression of the latter was confirmed with western blot  (inset). Renilla  (RLUC) and Firefly  (FLUC) luciferase  activities were simultaneously measured in leaf  extracts at 48 h post- infiltration using the  dual-luciferase  system  (n=4). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (***P&lt;0.001) was revealed by Student's t-test. The experiment was repeated more than 3 times with similar results."
NCBI gene IDs:
"FLUC: 
luciferase: 
RLUC: 
xopQ: 
ATG8a: 828287
NBR1: 828571"
Figure legend: "(D)  RT-qPCR analysis of NbATG8-1.1/1.2, NbATG8-2.1/2.2 and NbJoka2 transcript levels upon challenge of N. benthamiana plants with  Xcv ΔxopQ for 1 and 2 dpi compared to mock infected plants. Values represent expression relative to mock control of respective time point  and were normalized to actin. Values are biological replicates (n=4). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (*P &lt; 0.05, **P &lt; 0.01, *** P &lt; 0.001) was revealed by Student's t-test."
NCBI gene IDs:
"actin: 
ATG8: 
Joka2: 
xopQ: "
Figure legend: "(E) Bacterial density in leaves  of N. benthamiana infected with Xcv in the presence or absence autophagy suppressor AIMp-RFP. Leaves were syringe-infiltrated with OD600 = 0.0004, and colony-forming units  were counted at 6 dpi. Compared to empty vector control (EV), AIMp  expressing plants (n=6) harbour significantly more bacteria. Bacterial growth was repeated with the same result in 12 plants over two independent experiments. Red and yellow data points indicate independent repeats of the experiment. Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (***P &lt; 0.001) was revealed by Student's t-test. Expression of RFP-AIMp  was verified at 6 dpi with an anti-RFP blot  (inset)."
NCBI gene IDs:
"RFP: 
AIMp: 9461793"
Figure legend: "(A) RLUC-ATG8a  or RLUC-NBR1 constructs were coexpressed with internal control FLUC  in N. benthamiana. XopL  or GFP constructs were co-infiltrated. RLUC  and FLUC  signals were simultaneously measured in leaf  extracts at 48 h post- infiltration using the dual-luciferase  system. Values represent the ratio of RLUC-ATG8a  and FLUC  activities to the mean of control (n=4). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (P &lt; 0.01) was shown by Student's t-test. The experiment was repeated more than 3 times by with similar results."
NCBI gene IDs:
"FLUC: 
GFP: 
RLUC: 
XopL: 
ATG8a: 828287
NBR1: 828571"
Figure legend: "(B) Immunoblot  analysis of NBR1  and ATG8  protein levels in  N. benthamiana  plants transiently expressing GFP-XopL or GFP control at 2dpi verified with an anti-GFP  antibody (same blot was split for visualization purpose).  Ponceau Staining (PS) served as a loading control. The experiment was repeated at least three times with similar results."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(C) Immunoblot  analysis of ATG8  protein levels in  N. benthamiana plants transiently expressing XopL  or GUS  control at 2dpi after ConA  or DMSO treatment. Expression  of GFP-XopL  was verified with an anti-GFP antibody, while expression  of GUS-HA  was confirmed with an anti-HA  antibody. Ponceau Staining (PS) served as a loading control. The experiment was repeated twice with similar results."
NCBI gene IDs:
"XopL: 
GUS: 946149"
Figure legend: "(D) GFP-ATG8g-labeled autophagosomes  were quantified from plants infected with Xcv  or Xcv ΔxopL at 2 dpi in the presence or absence of ConA. Puncta were calculated from z-stacks (15) of n=12 individuals using ImageJ. Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (** P &lt; 0.01, *** P &lt; 0.001) was determined by one way ANOVA. The experiment was repeated twice with similar results."
NCBI gene IDs:
"xopL: "
Figure legend: "(E) Immunoblot  analysis of NBR1  and ATG8  protein levels in Xcv ΔxopQ, Xcv  ΔxopQ ΔxopL or mock infected N. benthamiana plants at 2dpi. Ponceau Staining (PS) served as a loading control. The experiment was repeated twice with similar results."
NCBI gene IDs:
"xopL: 
xopQ: "
Figure legend: "(F) RLUC-ATG8a  or RLUC-NBR1  constructs were coexpressed with internal control FLUC  in N. benthamiana. Xcv ΔxopQ and Xcv ΔxopQ  ΔxopL were co-infiltrated with Agrobacteria  containing the respective constructs. RLUC  and FLUC  activities were simultaneously measured in leaf  extracts at 48 h post- infiltration using the  dual-luciferase  system. Values represent the ratio of RLUC-ATG8a  and FLUC  activities (n=4). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance comparing Xcv ΔxopQ and Xcv ΔxopQ ΔxopL values (***P &lt; 0.001) was revealed by Student's t-test. The experiment was repeated 3 times with similar results."
NCBI gene IDs:
"FLUC: 
RLUC: 
xopL: 
xopQ: 
ATG8a: 828287
NBR1: 828571"
Figure legend: "(A) Interaction  of XopL  with SH3P2  in yeast two-hybrid assays. XopL  fused to the GAL4  DNA-binding   domain was expressed in combination with SH3P2  fused to the GAL4  activation domain (AD) in  yeast strain Y190. Cells were grown on  selective media before a LacZ  filter assay was performed. pSV40/p53  served as positive control, while the empty AD or BD vector served as negative control.  NtSH3P2 = Nicotiana tabacum SH3P2. -LT = yeast   growth on medium without Leu  and Trp, -HLT = yeast   growth on medium lacking His, Leu, and Trp, indicating expression of the HIS3  reporter gene. LacZ, activity of the lacZ  reporter gene."
NCBI gene IDs:
"HIS3: 
LacZ: 
lacZ: 
GAL4: 855828
p53: 716170"
Figure legend: "(B) Coimmunoprecipitation  of GFP-XopL  with AtSH3P2-HA. GFP-XopL  or GFP  were transiently coexpressed with AtSH3P2-HA   in leaves  of N. benthamiana. After 48 h, total proteins (Input) were subjected to immunoprecipitation  (IP) with GFP-Trap beads, followed by immunoblot  analysis using either anti-GFP  or anti-HA  antibodies. GFP blot  was split for visualization purpose. AtSH3P2 = Arabidopsis thaliana SH3P2. Two repetitions with similar results have been conducted."
NCBI gene IDs:
"GFP: 
HA: 
XopL: 
SH3P2: 829618"
Figure legend: "(E) Total proteins were extracted 48 hpi with  A. tumefaciens harboring the respective GFP-XopL, HA-XopL  and SH3P2-GFP expression  constructs. SH3P2-GFP  protein levels (lower band) were detected using an anti-GFP antibody. Expression   of the XopL was verified using an anti-HA  or anti-GFP  antibody. Expression  of GUS-HA  served as a control. Ponceau S staining serves as a loading control. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
HA: 
XopL: 
SH3P2: 829618"
Figure legend: "(F) Growth  of Xcv and Xcv  ΔxopL strains in  roq1 N. benthamiana plants silenced for SH3P2  (pTRV2-SH3P2) compared to control plants (pTRV2). Leaves  were dip-inoculated with a bacteria suspension at OD600 = 0.2 and bacteria were quantified at 3 and 6 dpi. Experimental repeats are indicated by data points in red (n=8) and yellow (n=6) data points. Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Different letters indicate statistically significant differences (P &lt; 0.05) as determined by one-way ANOVA."
NCBI gene IDs:
"xopL: 
SH3P2: 107790934"
Figure legend: "(A) SH3P2-GFP   was transiently coexpressed together with GUS-HA  and GFP-XopL  in N. benthamiana using agroinfiltration. At 42 hpi, 200 μM MG132 was infiltrated into  A. tumefaciens-inoculated leaves, and leaf  material was collected 48 hpi. Expression   of SH3P2-GFP  (lower band) and GFP-XopL  (upper band) was detected using an anti-GFP  antibody. GUS-HA expression   was confirmed with an anti-HA  antibody.  Ponceau S staining serves as a loading control. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
HA: 
XopL: 
SH3P2: 829618
GUS: 946149"
Figure legend: "(C) SH3P2-GFP was transiently coexpressed together with GFP, GFP-XopL  and GFP-XopL ΔE3 in N. benthamiana using agroinfiltration. GFP protein levels were detected with an anti-GFP  antibody. Ponceau S staining serves as a loading control. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(D) Immunoblot  analysis of ATG8  protein levels in N. benthamiana plants transiently expressing GFP-XopL, GFP-XopL ΔE3 or GFP  control at 2dpi. Expression of binary constructs was verified with an anti-GFP  antibody. GFP blot  was split for visualization purpose. Ponceau Staining (PS) served as a loading control. The experiment was repeated twice with similar results."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(E) RLUC-ATG8a  constructs were coexpressed with internal control FLUC  in N. benthamiana. GFP-XopL, GFP-XopL ΔE3 or GFP  control were co-infiltrated together with RLUC/FLUC  mixture. Renilla  and Firefly luciferase  activities were simultaneously measured in leaf  extracts at 48 hpi using the  dual-luciferase system. Values represent the ratio of RLUC-ATG8a  and FLUC  activities (n=4). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Statistical significance (* P &lt; 0.5, ** P &lt; 0.01) was revealed by Student's t-test. The experiment was repeated 3 times."
NCBI gene IDs:
"FLUC: 
GFP: 
luciferase: 
RLUC: 
XopL: 
ATG8a: 828287"
Figure legend: "(A) GFP-XopL was coexpressed with GFP  or AIMp-RFP. Proteins were separated by SDS-PAGE  and detected by immunoblotting  using the indicated antibodies. GFP blot  was split for visualization purpose. Ponceau Staining (PS) served as a loading control. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
RFP: 
XopL: 
AIMp: 9461793"
Figure legend: "(C)  FRET FLIM  measurements of GFP-Joka2  and RFP-XopL  in  N. benthamiana leaves. The freeRFP construct served as a negative control and RFP-ATG8E  (n = 9) as a positive control. Scattered points show individual data points, color indicates biological repeats. The lifetime  (in ns) of GFP-Joka2  (donor, n = 41) was significantly reduced in the presence of RFP-XopL  (n = 40) but not in the presence of freeRFP (n = 35). Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Significant differences were calculated using Wilcoxon rank sum test, with significantly different groups denoted by different letters. The experiment was repeated three times with similar results."
NCBI gene IDs:
"RFP: 
XopL: 
ATG8E: 819125"
Figure legend: "(D) Immunoprecipitation  (IP) of GFP-XopL  reveals association with NBR1. Immunoblots  of input and IP  samples from N. benthamiana plants transiently expressing GFP or GFP-XopL  were probed with anti-GFP  and anti-NBR1 antibodies. GFP blot  was split for visualization purpose."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(E) Immunoprecipitation  (IP) of GFP-XopL  and GFP-XopL ΔE3 reveals association with NBR1. Immunoblots  of input and IP  samples from  N. benthamiana  plants transiently expressing GFP, GFP-XopL  and GFP-XopL ΔE3 were probed with anti-GFP  and anti-NBR1  antibodies. GFP blot  was split for visualization purpose."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(F) GFP-XopL  was transiently expressed in pTRV2, pTRV2-Joka2  and N. benthamiana  WT plants. Expression   of binary constructs was verified with an anti-GFP  antibody. Joka2  silencing was verified using an anti-NBR1  antibody. Ponceau Staining (PS) served as a loading control. The experiment was repeated twice with similar results."
NCBI gene IDs:
"Joka2: "
Figure legend: "(G) Growth  of Xcv ΔxopQ in N. benthamiana  plants silenced for Joka2  (pTRV2-Joka2) compared to control plants (pTRV2). Leaves  were dip-inoculated with a bacteria suspension at OD600 = 0.2. and bacteria were quantified at 3 and 6 dpi. Red, blue, and green data points represent repeats of the experiments. Middle horizontal bars of boxplots represent the median, the bottom and top represent the 25th and 75th percentiles, whiskers extend to at most 1.5 times the interquartile range. Significant differences were calculated using Student's t-test and are indicated by: **, P &lt; 0.01. The experiment was repeated three times with similar trends."
NCBI gene IDs:
"Joka2: 
xopQ: "
Figure legend: "(H) GFP-XopL, GFP-XopL ΔE3 were transiently expressed in N. benthamiana. RFP-AIMp  was co-infiltrated to stabilize both XopL  variants. Samples were taken 48 hpi, and total proteins (Input) were subjected to immunoprecipitation  (IP) with GFP-Trap beads, followed by immunoblot  analysis of the precipitates using either anti-GFP  or anti-ubiquitin  antibodies. GFP  served as a negative control. RFP-AIMp expression was verified by an anti-RFP  antibody. GFP blot  was split for visualization purpose. Asterisk indicates the GFP-XopL  full-length protein. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
RFP: 
XopL: 
AIMp: 9461793"
Figure legend: "(I) GFP-XopL  was transiently expressed in N. benthamiana. Samples were taken 48 hpi, and total proteins (Input) were subjected to immunoprecipitation (IP) with the ubiquitin  pan selector, followed by immunoblot analysis of the precipitates using either anti-GFP  or anti-ubiquitin  antibodies. GFP  served as a control. Asterisk indicates the GFP-XopL  full-length protein. GFP blot  was split for visualization purpose. The experiment was repeated two times with similar results."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(J) Immunoblot  analysis GFP-XopL  and GFP-XopLK191A at 1 and 2 dpi using an anti-GFP  antibody. Ponceau Staining (PS) served as a loading control. The experiment was repeated three times with similar results."
NCBI gene IDs:
"GFP: 
XopL: "
Figure legend: "(K) HA-XopL  and GFP-Joka2  and its variants were transiently expressed in N. benthamiana. Samples were taken 48 hpi, and total proteins (Input) were subjected to immunoprecipitation  (IP) with GFP-Trap beads, followed by immunoblot  analysis of the precipitates using either anti-GFP, anti-ubiquitin  and anti-NBR1  antibodies. GFP  served as a control."
NCBI gene IDs:
"GFP: 
HA: 
Joka2: 
XopL: "
Figure legend: "C. MLE-12 AECs incubated with MH-S EVs infected with PR/8 influenza. Replication was quantified by RT-qPCR at 12 h post infection. Data represent mean M gene transcripts normalized to β actin from 3 independent experiments."
NCBI gene IDs:
"β actin: 
M: 23308107"
Figure legend: "E Total lung RNA was isolated from mice on day 1 post infection and virus was quantified by RT-qPCR. Data represent mean M gene transcripts from individual mice normalized to β actin from 3 independent experiments (4-5 mice per group)."
NCBI gene IDs:
"β actin: 
M: 23308107"
Figure legend: "A. MLE-12 AECs co-incubated with the listed WT strains (MOI = 0.4) plus AM-EVs (grey bars, EV:cell = 1). Data represent mean M gene transcripts at 12 h post infection relative to the mean of the corresponding untreated strain (white bars), each normalized to β actin from 2-4 independent experiments per strain."
NCBI gene IDs:
"β actin: 
M: 23308107"
Figure legend: "MLE-12 cells were incubated with WT PR/8 + AM-EVs at 4 °C (to permit surface binding but not entry) for 1 h, washed, and processed for (B) RNA isolation (B) Data represent mean M gene transcripts relative to the means of the corresponding untreated condition, each normalized to β actin from 3 independent experiments."
NCBI gene IDs:
"β actin: 
M: 23308107"
Figure legend: "C. RT-qPCR analysis of relative mRNA expression of Pde10a in BAT, peri-ovarian visceral white adipose tissue (VAT) and inguinal subcutaneous white adipose tissue (SAT) of lean mice (n = 5). ** P = 0.0063 (SAT vs VAT), ** P = 0.0011 (SAT vs BAT) and ** P = 0.0037 (VAT vs BAT) using unpaired 2-tailed Student's t test."
NCBI gene IDs:
"Pde10a: 23984"
Figure legend: "E RT-qPCR analysis of relative mRNA expression of thermogenic genes in SAT, VAT and BAT after treatment of lean mice treated with MP-10 (30 mg/kg) (n=7) or vehicle control (n = 6). **** P &lt; 0.0001 (Pgc1alpha in VAT), ** P = 0.0012 (Ucp1 in VAT), ** P = 0.0071 (Pgc1alpha in BAT) and * P = 0.0486 (Ucp1 in BAT) using unpaired 2-tailed Student's t tests."
NCBI gene IDs:
"Pgc1alpha: 19017
Ucp1: 22227"
Figure legend: "Representative fused PET/MRI images of the transverse view of the thoracic region of lean (n = 5), diet induced obese (DIO) (n = 4) and leptin deficient (ob/ob) (n = 5) mice that received the PDE10A radioligand [18F]-AQ28A. Interscapular brown adipose tissue (BAT) in each image is highlighted by the crosshairs. The mean standardized uptake (SUV) value of [18F]-AQ28A in BAT throughout the dynamic scan was calculated with associated area under the curve (AUC). * P = 0.0128 (lean vs DIO) and ** P = 0.0031 (lean vs ob/ob) using unpaired 2-tailed Student's t test."
NCBI gene IDs:
"ob: 16846"
Figure legend: "G. RT-qPCR analysis of relative mRNA expression of candidate genes in inguinal subcutaneous white adipose tissue (SAT), periovarian visceral white adipose tissue (VAT) and interscapularbrown adipose tissue (BAT) (n = 8 per group) *** P = 0.0004 (Pgc1alpha VAT), ** P = 0.0036 (Ucp1 VAT), ** P = 0.0068 (Cidea VAT), * P = 0.0411 (Tmem26 VAT) and ** P = 0.0070 (Pgc1alpha BAT) using unpaired 2-tailed Student's t tests."
NCBI gene IDs:
"Cidea: 12683
Pgc1alpha: 19017
Tmem26: 327766
Ucp1: 22227"
Figure legend: "A. RT-qPCR analysis of relative mRNA expression of thermogenic genes in cultured primary human brown adipocytes in response to chronic treatments (8 hours) with DMSO (control), MP-10 (1nM and 100nM), cyclic AMP (cAMP - 10µM), cyclic GMP (cGMP - 10µM) and cAMP + cGMP (cA + cG - 10µM (n = 4 separate cultures). * P = 0.0278 vs (100nM MP-10 vs control; Pgc1alpha), **P = 0.0097 (10µM cA + cG vs control; Pgc1alpha), ** P = 0.0063 (1nM MP-10 vs control; Ucp1), **** P &lt; 0.0001 (100nM MP-10 vs control; Ucp1), ## P = 0.0017 (1nM MP-10 vs 100nM MP-10; Ucp1), §§§§ P &lt; 0.0001 (10µM cAMP vs 100nM MP-10; Ucp1), §§§§ P &lt; 0.0001 (10µM cGMP vs 100nM MP-10; Ucp1), §§§§ P &lt; 0.0001 (10µM cA + cG vs 100nM MP-10; Ucp1), * P = 0.0112 vs (100nM MP-10 vs control; Cidea), *** P = 0.0007 (1nM MP-10 vs control; Dio2), **** P &lt; 0.0001 (100nM MP-10 vs control; Dio2), # P = 0.0267 (1nM MP-10 vs 10µM cAMP; Dio2), §§§ P = 0.0001 (10µM cAMP vs 100nM MP-10; Dio2), §§§ P = 0.0007 (10µM cGMP vs 100nM MP-10; Dio2), * P = 0.0232 (10µM cA + cG vs control; Dio2) §§ P = 0.0058 (10µM cA + cG vs 100nM MP-10; Dio2), * P = 0.0416 (10µM cGMP vs control; Prdm16) and * P = 0.0105 (10µM cA + cG vs control; Prdm16) using one-way analysis of variance (ANOVA) with Tukey's post-hoc test.B. RT-qPCR analysis of relative mRNA expression of thermogenic genes in cultured primary human white adipocytes in response to chronic treatments (8 hours) with DMSO (control), MP-10 (1nM and 100nM), cyclic AMP (cAMP - 10µM), cyclic GMP (cGMP - 10µM) and cAMP + cGMP (cA + cG - 10µM (n = 4 separate cultures). * P = 0.0186 vs (1nM MP-10 vs control; Ucp1), **** P &lt; 0.0001 (100nM MP-10 vs control; Ucp1), ## P = 0.0016 (1nM MP-10 vs 100nM MP-10; Ucp1), *** P = 0.0003 (10µM cAMP vs control; Ucp1), §§§ P = 0.0002 (10µM cGMP vs 100nM MP-10; Ucp1) and *** P = 0.0003 (cA + cG vs control; Ucp1) using one-way analysis of variance (ANOVA) with Tukey's post-hoc test."
NCBI gene IDs:
"Cidea: 1149
Dio2: 1734
Pgc1alpha: 10891
Prdm16: 63976
Ucp1: 7350"
Figure legend: "Egg chamber elongation is unaffected by reduced cell proliferation. String-shRNA expressed for 24 hours alters epithelial shape and cell number (A Chambers shown in (A) have approximately equal widths, with the cross-sectional area corresponding to developmental stage 6. Scale bars = 20 μm. Number of chambers analyzed: w1118 1.0-1.2 n=24,1.2-1.4 n=14,1.4-1.6 n=18 String-shRNA 1.0-1.2 n= 11 ,1.2-1.4 n=10,1.4-1.6 n=15. Bars represent mean and standard deviation"
NCBI gene IDs:
"String: 43466"
Figure legend: "Egg chamber elongation is unaffected by reduced cell proliferation. String-shRNA expressed for 24 hours alters epithelial shape and cell numbe but not the relationship between egg chamber aspect ratio and cross-sectional area, which is quantified in (B) Number of chambers analyzed: w1118 1.0-1.2 n=24,1.2-1.4 n=14,1.4-1.6 n=18 String-shRNA 1.0-1.2 n= 11 ,1.2-1.4 n=10,1.4-1.6 n=15. Bars represent mean and standard deviation"
NCBI gene IDs:
"String: 43466"
Figure legend: "C, D) Cell proliferation is necessary for normal tissue morphology Follicle cell shape regularity (C) and polygonality (D) measurements in control and String-shRNA egg chambers of different aspect ratios. These measurements were derived from at least three egg chambers from at least three flies per AR range. Bars represent mean and standard deviation. Statistical significance for cell side distributions was calculated using a chi-squared test. Number of cells used for analysis: w1118 1.0-1.2 n= 23, 1.2-1.4 n=114, 1.4-1.6 n=160; String-shRNA 1.0-1.2, n=6, 1.2-1.4 n=19, 1.4-1.6 n=17"
NCBI gene IDs:
"String: 43466"
Figure legend: "Epithelial cell size and shape is affected by decreased proliferation. Cell outlines are marked with Basigin::YFP. Scale bars =­ 5 μm. Cells become more aligned with the tissue elongation axis when proliferation is stopped using String-shRNA (C). Number of cells analyzed: w1118 1.0-1.2 n= 14, 1.2-1.4 n=114, 1.4-1.6 n=160; String-shRNA 1.0-1.2, n=10, 1.2-1.4 n=19, 1.4-1.6 n=17. Statistical significance calculated using a Mann-Whitney test. Data information: Bars represent mean and standard deviation."
NCBI gene IDs:
"String: 43466"
Figure legend: "D, E) The follicular epithelium 'thins out' over the egg chamber equator when proliferation is blocked. Cartoon demonstrates the tissue cell shape observed in the follicular epithelium in control (w1118) and String-shRNA treated egg chambers. Quantification of the phenomenon shown in (D) reveals that cells are shorter over the equator than at the anterior pole in elongated egg chambers when proliferation is blocked. Statistical significance calculated using unpaired t-test with Welch's correction. Number of cells analyzed: w1118 1.0-1.2 n= 21, 1.2-1.4 n=11, 1.4-1.6 n=10; String-shRNA 1.0-1.2, n=9, 1.2-1.4 n=11, 1.4-1.6 n=13. Data information: Bars represent mean and standard deviation."
NCBI gene IDs:
"String: 43466"
Figure legend: "F) Recoil is observed when cuts are made in apical cortex with a 2-photon laser with egg chambers in saggital view. Images show cortex before and after cutting. Scale bars = 10 μm. Yellow arrows indicate edges of ablated region. G) Recoil is higher when cuts are made at the apical cortex at the equator of egg chambers, rather than at the poles. Quantification of experiment shown in (F). Statistical significance tested by Mann-Whitney test. Number of laser cuts: equator n=30, pole n=20. Data information Laser ablation experiments performed on tissue from sqhAX3, sqh::GFP flies."
NCBI gene IDs:
"sqh: 31554"