EMBO/SourceData huggingface dataset:
Figure legend: "(I) Effect of ANO8-YFP on myc-STIM1 and Orai1-HA at the ER/PM junction. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"YFP: 
ANO8: 57719"
Figure legend: "(M) Example EM images recorded from HEK cells transfected with empty vector (left) or ANO8 (right). Red arrows mark ER/PM junctions. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(N, O) The size (N) and density (O) of ER/PM junctions in vector-transfected (control) and ANO8-transfected cells. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(A, B) Knockdown of ANO8 (siA8) reduced CRAC current in cells transfected with Orai1 (O1) and STIM1 (S1) and buffered with 3 mM EGTA. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed and difference were analyzed by unpaired t test."
NCBI gene IDs:
"A8: 57719
ANO8: 57719
O1: 84876
Orai1: 84876
S1: 6786
STIM1: 6786"
Figure legend: "(C) Knockdown of ANO8 reduces the native store-dependent Ca2+ influx measured in store depleted cells by Ca2+ add-back. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(D,E) Knockdown of ANO8 reduced the number of store-dependent STIM1 puncta at the TIRF plane in cells expressing STIM1 and Orai1. Panel (D) shows representative images and (E) is the summary of 7 experiments. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(F, G) Current was measured with pipette solution contacting the fast and strong Ca2+ buffer 10 mM BAPTA in HEK cells transfected with STIM1, Orai1, and with (red) or without ANO8 (black). Panel (G) shows the increase in current density at peak current. Note the prominent current inactivation in the presence of ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719
Orai1: 84876
STIM1: 6786"
Figure legend: "(H) Knockdown of SARAF (red) in wild-type cells had no effect on current inactivation in the presence of 10 mM BAPTA. (I) Knockdown of SARAF did not prevent the ANO8-dependent current inactivation in the presence of 10 mM BAPTA. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed and difference were analyzed by unpaired t test. "
NCBI gene IDs:
"ANO8: 57719
SARAF: 51669"
Figure legend: "(A, B) Depletion of plasma membrane PI(4,5)P2 with the FRB/FKBP system prevented the ANO8-mediated increase in STIM1-Orai1 current measured in pipette solutions containing 3 mM EGTA (A) or 10 mM BAPTA (B). Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(C) HEK cells transfected with ANO8-YFP, STIM1-CFP Orai1-HA and FRB and mCherry-FKBP PI(4,5)P2 depleting constructs were treated with 0.2 µM rapamycin (rapa) for 5 min and then with 25 µM CPA for 10 min before fixation and imaging by confocal microscopy. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"CFP: 
FKBP: 
FRB: 
HA: 
mCherry: 
YFP: 
ANO8: 57719
Orai1: 84876
STIM1: 6786"
Figure legend: "(D) Mutating ANO8 R948, R950, R951 in RPRRP, a region that is predicted to include an ANO8 PI(4,5)P2 binding site, prevented increased STIM1-Orai1 current and inactivation. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(E) Mutating ANO8 R949,950,951 to Q reduced translocation of ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(A) Plot of the slope of inactivation as a function of current density in the presence (red) and absence of ANO8 (black). Increasing current density was obtained by varying external Ca2+ between 2-50 mM. The results are mean±s.e.m and difference were analyzed by unpaired t test. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(D, E) Experimental protocol and conditions except that cells were also transfected with ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(F, G) HEK cells expressing STIM1, Orai1, and ANO8 were used to measure current in pipette solution containing 10 mM BAPTA and with (red) or without (black) 100 µM IP3 or 10 mM of the ER Ca2+ chelator TPEN (green). The current density (F) and the normalized current (G) are shown, illustrating the delay in current inactivation by IP3 and TPEN. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed."
NCBI gene IDs:
"Orai1: 84876
STIM1: 6786"
Figure legend: "(A, B): ER Ca2+ content was measured with ER-GECO1 in the periphery (A) and the center (B) or cells transfected with M3 receptors and with (red, green) or without (black, blue) ANO8. Receptor mediated store depletion was initiated by stimulating the cells with 0.5 mM carbachol in Ca2+-free solution. Cell stimulation was terminated with 10 µM atropine and ER Ca2+ uptake was initiated by perfusing the cells with a solution containing 5 mM Ca2+. The fluorescence was measured as F/F0 and normalized to the initial fluorescence in the presence of ANO8. Panel (C) shows traces of ER Ca2+ uptake at expanded time scale in the periods marked by rectangular in (A and B) and the averaged slopes of ER Ca2+ influx and (D) shows the averaged slope of Ca2+ release at the cell periphery (Peri) and cell center (Center). In (C) the traces were aligned along the Y axis to better show the difference in uptake rate. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(E) Enhancement by ANO8 of the Co-IP of expressed STIM1 with PMCA4a and SERCA2 and of Orai1 with SERCA2 in resting and store depleted cells. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(F) Effect of ANO8 expression on the Co-IP of the native SERCA2 and STIM1 in resting and store depleted cells. The columns are the mean±s.e.m of 3 experiments. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(G) FRET ratio between STIM1 and SERCA2 in response to store depletion and its enhancement by ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(J) Effect of ANO8 on the Co-IP of the native STIM1 and IP3Rs. The columns are the mean±s.e.m of 3 experiments. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(K) FRET ratio between expressed STIM1 and IP3R3 in response to store depletion and its enhancement by ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(L) FRET ratio between expressed STIM1 and PMCA4 in response to store depletion and its enhancement by ANO8. Data information: The first number in parenthesis indicates the number of similar experiments performed and the second number is the number of cells analyzed. All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(A-D) HeLa cells transfected with GFP (black) or ANO8 (red) were stimulated with 0.5 and then 1 µM ATP to activate the native P2Y2 receptors. The oscillations were analyzed in terms of the % of responding cells (B), oscillation frequency (C), and the amplitude of the Ca2+ signal (D). Data information: All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"GFP: 
ANO8: 57719"
Figure legend: "(E-H): HeLa cells were treated with scrambled siRNA (black) or siANO8 (red) and were stimulated with 1 and then 5 µM ATP. The oscillations were analyzed in terms of the % of responding cells (F), oscillations frequency (G), and the amplitude of the Ca2+ signal (H). Data information: All results are given as mean±s.e.m of the indicated number of experiments or cells analyzed, and difference were analyzed by unpaired t test."
NCBI gene IDs:
"ANO8: 57719"
Figure legend: "(A) RT-qPCR analysis of NimB1,  NimB2, NimB3, NimB4 and NimB5  transcripts, normalized to RpL32, from carcass, macrophages, fat body  and  gut  of wild type (w1118) L3 wandering larvae. Data are represented as mean ± SD from three independent experiments."
NCBI gene IDs:
"NimB1: 34813
NimB2: 260645
NimB3: 3885611
NimB4: 34814
NimB5: 34815
RpL32: 43573"
Figure legend: "(A) Representative  confocal imaging of wild type (w1118), NimB4 sk2 and apoptosis-null NimB4 sk2; Df(3L) H99 embryonic macrophages  at stage 16 of development; ventral and lateral view; Tissues were co-stained with Dcp-1 (red, corresponding to apoptotic corpses) and anti-SIMU  (green, corresponding to macrophages) antibodies. The arrows show the presence of apoptotic cells  inside embryonic macrophages. Scale bars = 20 μm"
NCBI gene IDs:
"NimB4: 34814"
Figure legend: "(C) Representative projections from confocal  stacks of entire third instar wild type (w1118) and NimB4 sk2 larval brains stained  with anti-Draper  (green) and anti-Dcp1 (red). The arrows show the presence of  apoptotic cells  in the central area of  NimB4 sk2 larval brain. Scale bars = 100 μm"
NCBI gene IDs:
"NimB4: 34814"
Figure legend: "(A) Representative  confocal imaging of wild type (w1118), NimB4 sk2 and draper macrophages incubated with fluorescently-labeled apoptotic cells (red, CellTraceTM Red) on ice for 1h and stained  with AlexaFluorTM 488 phalloidin  (green). Scale bar: 10 μm. (B) Quantification of the  number  of  apoptotic cells (red)  binding to the  macrophages  (green). Values from three independent experiments are represented as mean ± SD (*** P&lt;0.001 by ANOVA test followed by post hoc Dunnett's multiple comparison tests. ns: not significant). "
NCBI gene IDs:
"draper: 38218
NimB4: 34814"
Figure legend: "(D) Ex vivo phagocytosis assay  using Alexa555 fluorescent apoptotic bodies. Wild type, NimB4sk2, NimB4 genomic rescue (NimB4sk2, [NimB4]), draper and NimC11,  eater1 macrophages from L3 wandering were incubated with Alexa555 fluorescent apoptotic bodies for 30, 60 or 120 min at room temperature. Phagocytosis  was quantified by flow cytometry. Data are represented as mean ± SD from three independent experiments (* P&lt;0.05,** P&lt;0.01, **** P&lt;0.0001 by ANOVA test followed by post hoc Dunnett's multiple comparison tests ns: not significant). (E) Ex vivo phagocytosis assay  using  AlexFluor488 S.aureus Bioparticles. Wild type, NimB4sk2 and NimC11, eater1 macrophages from L3 wandering were incubated with  bioparticles for 30, 60 or 120 min at room temperature. Phagocytosis  was quantified by  flow cytometry. Data are represented as mean ± SD from three independent experiments (**** P&lt;0.0001 by ANOVA test followed by post hoc Dunnett's multiple comparison tests. ns: not significant). "
NCBI gene IDs:
"draper: 38218
eater: 43262
NimB4: 34814
NimC1: 34816"
Figure legend: "(A) Representative fluorescence microscopy images of wild type (w1118),  NimB4sk2, draper, eater1 and croquemort△ third instar larvae macrophages stained with LysoTracker Red (live imaging). Overlay of fluorescence and differential interference contrast microscopy  (DIC). Scale bar = 10 μm. (B) Mean fluorescence intensity after  staining  with LysoTracker Red (live confocal imaging). Values from at least three independent experiments are represented as mean ± SD ( ** P&lt;0.01, **** P&lt;0.0001 by ANOVA test followed by post hoc Dunnett's multiple comparison tests). "
NCBI gene IDs:
"croquemort: 33219
draper: 38218
eater: 43262
NimB4: 34814"
Figure legend: "(C) Representative transmission electron micrographs of macrophages from wild type (top, w1118) and NimB4sk2 (bottom) L3 wandering larvae. Scale bar: 2 μm (D) Quantification of the number of vesicles  per  macrophages in the electron micrographs. Values from at least three independent experiments are represented as mean ± SD (**** P&lt;0.0001 by Mann-Whitney test). "
NCBI gene IDs:
"NimB4: 34814"
Figure legend: "(E) Representative confocal imaging of Rab7EYFP immunostaining in wild type (w1118) and NimB4 sk2 macrophages. Tissues were stained with anti-GFP (red, Rab7), counterstained with  phalloidin (gray) and  DAPI  (blue). The arrows indicate the enlarged vesicles  are decorated with Rab7EYFP in the NimB4sk2 macrophages. Scale bar = 10 μm."
NCBI gene IDs:
"NimB4: 34814"
Figure legend: "(A) Representative confocal imaging of localization  of  Lamp1-mcherry and LysoTracker Green in wild type (w1118), NimB4 sk2 and draper△5 macrophages (live imaging). The arrows indicate the colocalization (w1118) or the clustering (NimB4sk2 and draper△5) of  Lamp1-mcherry and LysoTracker Green signals. Scale bar: 10 μm. (B) Quantification of the  colocalization of Lamp1-mcherry with LysoTracker Green, as measured by Pearson's correlation coefficient between the two signals. Values from at least five independent experiments are represented as mean ± SD (* P&lt;0.05 by ANOVA test followed by post hoc Dunnett's multiple comparison tests). "
NCBI gene IDs:
"draper: 38218
NimB4: 34814"
Figure legend: "(C) Ex vivo phagocytosis assay using apoptotic cells labelled with  pHrodo™ Red. Wild type (w1118), NimB4sk2 and draper△5 macrophages from L3 wandering larvae were incubated with pHrodo™ Red apoptotic cells for 60 min at room temperature.  Phagocytosis was quantified by  flow cytometry. Data are represented as mean ± SD from three independent experiments (* P&lt;0.05 by ANOVA test followed by post hoc Dunnett's multiple comparison tests. ns: not significant). (D) Ex vivo phagocytosis assay using pHrodoTM Red S.aureus BioparticlesTM conjugates. Wild type, NimB4sk2 and NimC11, eater1 Hml∆-Gal4&gt;UAS-GFP macrophages from L3 wandering  larvae were incubated with  pHrodoTM Red S. aureus BioparticlesTM for 30, 60 or 120 min at room temperature. Phagocytosis was quantified by  flow cytometry. Data are represented as mean ± SD from three independent experiments (* P&lt;0.05by ANOVA test followed by post hoc Dunnett's multiple comparison tests. ns: not significant). "
NCBI gene IDs:
"GFP: 
draper: 38218
eater: 43262
Gal4: 855828
Hml: 39529
NimB4: 34814
NimC1: 34816"
Figure legend: "(A-B) Representative fluorescence microscopy images of wild type (A) or NimB4sk2 (B) macrophages expressing Rab5  (middle panel) or  Rab7 (right panel) driven by Hml∆-Gal4 and stained with the  LysoTracker Red  (live imaging). Overlay of fluorescence and DIC. Scale bar = 10 μm. (C) Quantification of the mean fluorescence intensity of  LysoTracker in macrophages from  larvae (confocal live imaging). Values from at least three independent experiments are represented as mean ± SD (** P&lt;0.01, **** P&lt;0.0001 by ANOVA test followed by post hoc Dunnett's multiple comparison tests. ns: not significant). "
NCBI gene IDs:
"Gal4: 855828
Hml: 39529
NimB4: 34814"
Figure legend: "Representative microscopic views of Ndrg4+/+ and Ndrg4-/- murine intestinal sections (n=4, 12 months of age) reveal an even distribution of alkaline phosphatase along the enterocyte brush border, and a similar number and distribution of Paneth cells (Lysozyme) in the small intestine; and neuroendocrine (Chromogranin A), Goblet (PAS+), and proliferating (Ki67) cells in the colon. Scale bars, 50 µm."
NCBI gene IDs:
"Ndrg4: 234593"
Figure legend: "Representative microscopic views of the myenteric plexus of Ndrg4+/+ and Ndrg4-/- murine colonic sections labeled with either TuJ1 and S100 (B, n=3) or HuC/D (C, n=6) did not reveal structural or organizational differences in the ganglionic network of Ndrg4+/+ and Ndrg4-/- mice. Scale bars, 100 µm. Quantification of the enteric neuronal cell number (HuC/D) shows a similar number of enteric neurons in the proximal and distal colon of Ndrg4+/+ and Ndrg4-/- mice (n=6)."
NCBI gene IDs:
"HuC/D: 16016
Ndrg4: 234593
S100: 100344971
TuJ1: 22152"
Figure legend: "In contrast, Ndrg4fl/fl and Ndrg4fl/fl-VillinCre mice have a similar bodyweight (I) and develop an equal number of adenomas (J) with an equivalent diameter (K,L). N=13 vs. 12 in I, n=13 vs. 11 in J and n=10 vs. 8 in K."
NCBI gene IDs:
"Cre: 2777477
Ndrg4: 234593
Villin: 22349"
Figure legend: "Compared to Ndrg4+/+ ENS-derived medium, addition of medium derived from Ndrg4-/- ENS cultures enhances the relative growth of intestinal epithelial organoids (E, IEOs) and accelerates the formation of new crypt buds per IEO in the course of five days (F)."
NCBI gene IDs:
"Ndrg4: 234593"
Figure legend: "Box plot showing the quantitative protein expression results of nanoLC-MS/MS analysis reveals the significantly higher presence of Nid1 and Fbln2 in the Ndrg4-/- compared to the Ndrg4+/+ ENS cell secretome (n=4; NSAF, normalized spectral abundance factor). Data are analyzed with R version 3.5.2 and the ibb R package. Each dot within the box plot represents the NSAF of an individual sample; the inside band reflects the median, and the bottom and top of the box the first and third quartile, respectively. The whiskers reflect the minimum and maximal values within 1.5× the interquartile range. NSAF values were compared using the Mann-Whitney U test."
NCBI gene IDs:
"Ndrg4: 234593"
Figure legend: "(E) The expression of GSC maintenance genes (aret, Mei-P26, egg, Myc and Hrb98DE) and differentiation genes (bam, bgcn, out, twin and Set1) along the primary branch (dotted circle line) in pseudotime."
NCBI gene IDs:
"bam: 43038
bgcn: 47873
aret: 34648
egg: 37962
Hrb98DE: 43385
Mei-P26: 45775
Myc: 31310
out: 32926
Set1: 3354971
twin: 42880"
Figure legend: "(C-C') Immunofluorescence staining with anti-TfIIA-S on wild type and nos-Gal4&gt;UAS-TfIIA-S-RNAi (negative control). The scale bar is 10 μm."
NCBI gene IDs:
"Gal4: 855828
nos: 42297
TfIIA-S: 42822"
Figure legend: "(E-E') Immunofluorescence staining with anti-eggpl on wild type and nos-Gal4&gt;UAS-eggpl-RNAi (negative control) ovary. The scale bar is 10 μm."
NCBI gene IDs:
"eggpl: 318223
Gal4: 855828
nos: 42297"
Figure legend: "(F) The overexpression of GFP (green) and eggpl in Sf9 cell line in vitro. The anti-eggpl (red) was used to detect the eggpl protein, and Hoechst (blue) was used to label the nucleus. The scale bar is 10 μm."
NCBI gene IDs:
"eggpl: 318223"
Figure legend: "(A-A') The mRNA in situ hybridization of eggpl (green) on wild type and nos-Gal4&gt;UAS-eggpl-RNAi (negative control) line. The scale bar is 10 μm. (B-C) Immunofluorescence staining with anti-α-Spectrin (red) and anti-Vasa (red) on eggpl-in situ (green) labeled tissues respectively. The scale bar is 10 μm. The dashed line indicates the eggpl-in situ (green) signals."
NCBI gene IDs:
"eggpl: 318223
Gal4: 855828
nos: 42297"
Figure legend: "(F and H) The mRNA in situ hybridization of eggpl (green) on nos-Gal4&gt;UAS-eggpl-RNAi (negative control) (F) and nos-Gal4&gt;UAS-eggpl-GFP lines (H). The scale bar is 10 μm. (G) The anti-GFP, anti-α-Spectrin (red) and anti-Bam (blue) were used to stain on eggpl::GFP knock-in line. The scale bar is 10 μm. (I) Double staining with eggpl in situ and anti-GFP on eggpl knock-in lines. The scale bar is 10 μm. The dashed line indicates the eggpl-in situ (green) signals."
NCBI gene IDs:
"GFP: 
eggpl: 318223
Gal4: 855828
nos: 42297"
Figure legend: "(K) The phenotypes of wild type, nos-Gal4&gt;UAS-eggpl-RNAi line and nos-Gal4&gt;UAS-eggpl::GFP line staining with anti-α-Spectrin (green), anti-pMad (red). The scale bar is 10 μm."
NCBI gene IDs:
"GFP: 
eggpl: 318223
Gal4: 855828
nos: 42297"