EMBO/SourceData huggingface dataset:
Figure legend: "G. Brigatinib treatment (0 or 150 nM) for 6h resulted in inhibition of ALK phosphorylation, and of activation of downstream signaling (ERK1/2), as well as MYCN expression. Cell lysates (Rosa26_Alkal2;Th-MYCN (#3540) and Alk-F1178S;Th-MYCN (#3456)) were immunoblotted with the indicated antibodies."
NCBI gene IDs:
"Alk: 11682
Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "A. Cells derived from tumours arising in Rosa26_Alkal2;Th-MYCN (#4953) and Alk-F1178S;Th-MYCN (#4938) mice are sensitive to both lorlatinib and brigatinib. The effect of increasing concentrations of each ALK TKI (as indicated) on cell confluence was analyzed by IncuCyte Live Cell Analysis. Data is presented as mean ± SEM from three independent experiments. *p&lt;0.05, **p&lt;0.005; two-tailed paired student t-test."
NCBI gene IDs:
"Alk: 11682
Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "B. Tumour volume changes over time for Rosa26_Alkal2;Th-MYCN mice treated with lorlatinib (10 mg/kg; twice daily) or vehicle control. Tumour volume was measured by ultrasound on Day 0 and 7, and by direct measurement at Day 14. Day 0 (lorlatinib n=7, Ctrl n=6), Day 7 (lorlatinib n=2, Ctrl n=5) and Day 14 (lorlatinib n=7, Ctrl n=6). Data shown represents mean ± SD. **p&lt;0.005; two-tailed unpaired student t-test."
NCBI gene IDs:
"Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "C. Rosa26_Alkal2;Th-MYCN animals treated with lorlatinib did not display any significant loss of body weight compared with vehicle controls. Data shown represents mean ± SD."
NCBI gene IDs:
"Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "D. Representative ultrasound images of tumours observed in Rosa26_Alkal2;Th-MYCN mice with annotated measurements at Day 0 and Day 7. Tumours arise in the retroperitoneal space ventral to the aorta, Ao."
NCBI gene IDs:
"Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "E. Representative MRI imaging of Rosa26_Alkal2;Th-MYCN tumours in response to lorlatinib at 4d and 14d."
NCBI gene IDs:
"Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "F. Rosa26_Alkal2;Th-MYCN tumours from lorlatinib or vehicle controls were analysed for pHis. A representative field of view for each tumour at 40x (175740μm2) were manually counted. Data shown represents mean ± 95% CI. p=0.0286; Mann-Whitney test."
NCBI gene IDs:
"Alkal2: 100294583
Rosa26: 14910
MYCN: 4613
Th: 25085"
Figure legend: "B. The BZLF1 m6A levels were measured using MeRIP-qPCR in CNE2EBV, Raji and B95-8 cells with latent EBV infection or lytic reactivation. TPA (30 ng/ml) and NaB (2 mM) were used to treat the cells for 24 h to reactivate EBV. The cellular RNA was harvested for MeRIP-qPCR assays. The fold enrichment was determined by calculating the 2-ΔCt of the MeRIP sample relative to the input sample. The mean value of the results in mock-treated cells was normalized to 1."
NCBI gene IDs:
"BZLF1: 3783744"
Figure legend: "M. The relative EBV production in C666 cells transfected with YTHDF1-specific siRNAs (siY1-1 and siY1-2) or the siNC control was measured by qPCR. The DNA from the cell-free culture supernatant was harvested at 24 h after siRNA transfection. The number of EBV copies in the supernatant of the cells was quantified by qPCR using specific primers targeting BamHI-W fragment region of EBV. The mean value of EBV copies in the siNC cells was normalized to 1."
NCBI gene IDs:
"Y1: 54915
YTHDF1: 54915"
Figure legend: "B. The relative mRNA levels of BZLF1 and BRLF1 in the latently infected or reactivated (24 h post NaB treatment) CNE2EBV cells transfected with the YTHDF1-specific siRNAs (siY1-1 and siY1-2) or the siNC control. After 24 h of siRNA transfection, CNE2EBV cells were treated with NaB (2 mM) or mock for 24 h, followed by qRT-PCR analysis. All the gene expression levels were normalized to the housekeeping gene ACTB."
NCBI gene IDs:
"ACTB: 
BRLF1: 3783727
BZLF1: 3783744
Y1: 54915
YTHDF1: 54915"
Figure legend: "D. The relative enrichment of the BZLF1 and BRLF1 mRNA in CNE2EBV cells with transfected with siYTHDF2, siYTHDF3 or siNC siRNA following EBV reactivation. After 24 h of siRNA transfection, CNE2EBV cells were treated with TPA (30 ng/ml) and NaB (2 mM) for 24 h. The RIP assays were performed using YTHDF1-specific antibody or IgG control in these cells. Fold enrichment was determined by calculating the 2-ΔCt of the RIP sample relative to the input sample."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744
YTHDF2: 51441
YTHDF3: 253943"
Figure legend: "E, F. The relative m6A and YTHDF1 enrichment of the BZLF1 (E) or BRLF1 (F) mRNAs measured in CNE2EBV cells transfected with the METTL14-specific siRNAs (siM14) or siNC control following EBV reactivation. After 24 h of siRNA transfection, CNE2EBV cells were treated with TPA (30 ng/ml) and NaB (2 mM) for 24 h, followed by MeRIP and RIP assays. The fold enrichment was determined by calculating the 2-ΔCt of the RIP sample relative to the input sample."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744
M14: 57721
METTL14: 57721"
Figure legend: "F. The interactions between endogenous YTHDF1 and ZAP, DDX17 or DCP2 were detected by endogenous IP assays in CNE2EBV cells transfected with YTHDF2-, YTHDF3-specific siRNAs or the siNC control. YTHDF1, YTHDF2, YTHDF3, ZAP, DDX17, and DCP2 were detected in the immunoprecipitated complexes using specific antibodies, respectively."
NCBI gene IDs:
"YTHDF2: 51441
YTHDF3: 253943"
Figure legend: "F. The relative mRNA levels of BZLF1 and BRLF1 in C666 cells transfected with YTHDF1-specific siRNAs and XRN1-specific siRNAs. The C666 cells were co-transfected with YTHDF1-specific siRNAs and XRN1-specific siRNAs for 24 h. The mRNA levels were determined using qPCR."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744
XRN1: 54464
YTHDF1: 54915"
Figure legend: "G. The relative enrichment of BZLF1 and BRLF1 mRNAs by ZAP, DDX17, or DCP2 in CNE2EBV cells following EBV reactivation. CNE2EBV cells were transfected with plasmids encoding myc-ZAP, myc-DDX17, myc-DCP2, or vector control. At 24 h post transfection, the cells were treated with TPA (30 ng/ml) and NaB (2 mM) for 24 h. The RIP assays were performed using Myc-beads. The fold enrichment was determined by calculating the 2-ΔCt of the RIP sample relative to the input sample."
NCBI gene IDs:
"BRLF1: 3783727
BZLF1: 3783744
DCP2: 167227
DDX17: 10521
ZAP: 56829"
Figure legend: "H. Determination of the EBV infection efficiency in HK1 cells transfected with the XRN1-specific siRNA or the siNC control. After 24 h of siRNA transfection, HK1 cells were infected with EBV and analyzed by FACS at 24 hpi."
NCBI gene IDs:
"XRN1: 54464"
Figure legend: "A. Maximum intensity projections across Z stacks of example images from indicated mESCs stained for L1 Tf RNA (red) combined with immunostaining for L1 ORF1p (green) and nuclei stained with DAPI (blue). The grey square marks position of the inset. White arrow heads point to cytoplasmic foci where L1 RNA and ORF1p co-localize. Scatter plot shows the number of co-localized L1 Tf-ORF1 foci in the cytoplasm per cell (n=275 WT, 304 Dicer_KO1, 311 Dicer_KO2 cells). Red arrow heads point to relatively larger sized L1 RNP foci. Bar graphs are mean values of percentage of cells with large L1 Tf-ORF1 foci co-localizing in the cytoplasm (n=3 technical replicates)."
NCBI gene IDs:
"Dicer: 192119
L1: 381591"
Figure legend: "B. Maximum intensity projections across Z stacks of example images from indicated mESCs immunostained for L1 ORF1p (red), MOV10 (green) and nuclei stained with DAPI (blue). The grey square marks position of inset in the zoomed image. White arrow heads point to cytoplasmic foci where L1 ORF1p and MOV10 co-localize. Scatter plot shows the number of co-localized ORF1-MOV10 foci in the cytoplasm per cell (n= 293 WT, 295 Dicer_KO1, 295 Dicer_KO2 cells). Red arrow heads point to relatively larger sized L1 ORF1-MOV10 foci. Bar graphs are mean values of percentage of cells with large ORF1-MOV10 foci co-localizing in the cytoplasm (n=3 technical replicates)."
NCBI gene IDs:
"Dicer: 192119"
Figure legend: "A. WT and Dicer_KO mESCs were treated with 0.5mM Sodium Arsenite (NaAsO2) for 20 minutes or left untreated prior to fixation with formaldehyde. Maximum intensity projections across Z stacks of example images from indicated mESCs immunostained for G3BP1 (red) and MOV10 (green) with nuclei stained with DAPI (blue)."
NCBI gene IDs:
"Dicer: 192119"
Figure legend: "B. Representative western blots showing low AGO2 protein levels in Dicer_KO as compared to WT mESCs (right side). LAMINB1 served as loading control. On the left, maximum intensity projections across Z stacks of example images from indicated mESCs stained for L1 Tf RNA FISH (red) combined with immunostaining for a resident protein of P-bodies, DDX6 (green) and nuclei stained with DAPI (blue). The grey square marks position of the inset. Yellow arrow heads point to cytoplasmic foci where L1 RNA and DDX6 protein co-localize."
NCBI gene IDs:
"Dicer: 192119
L1: 381591"
Figure legend: "B. Maximum intensity projections across Z stacks of example images from indicated mESCs immunostained for L1 ORF1p (green) combined with RNA FISH for L1 Tf RNA (red) and nuclei stained with DAPI (blue) (n=3 technical replicates)."
NCBI gene IDs:
"L1: 381591"
Figure legend: "C. Representative images of BlastR colonies stained with crystal violet blue of indicated cell lines is shown on the left. Cells were transfected with either mutant reporter plasmid (L1N21A) or retrotransposition competent reporter (L1WT) as shown in the scheme with timeline for the experiment on the top. Bar graphs on the right depict the average number of BlastR colonies (n=3 biological replicates)."
NCBI gene IDs:
"L1: 381591"
Figure legend: "A. Scheme and quantification of relative luminescence from luciferase assays validating post-transcriptional regulation of MOV10 by miRNAs in HEK293T cells (n=4 biological replicates)."
NCBI gene IDs:
"MOV10: 17454"
Figure legend: "B. Scheme and quantification of relative luminescence from luciferase assays in Hek293T cells to confirm specificity of post-transcriptional regulation of MOV10 by miRNAs with either wild-type (WT) or Mutant (MUT) reporters where the MicroRNA Response Element (MRE) for the depicted miRNAs in the 3'UTR of MOV10 were mutated (n=3 biological replicates)."
NCBI gene IDs:
"MOV10: 4343"
Figure legend: "D. Scheme and representative images from colony forming assays for plasmid based retrotransposition analysis where miRNA mediated downregulation of MOV10 in Drosha_KO mESCs is accompanied by an increase in L1 retrotransposition (n=3 biological replicates)."
NCBI gene IDs:
"Drosha: 14000
L1: 381591
MOV10: 17454"
Figure legend: "A. Scheme of experiment and representative images along with quantitation showing induction of L1 RNP aggregate formation upon ectopic MOV10 expression. Images are maximum intensity projections across Z stacks from indicated mESCs stained for L1 ORF1p (red) combined with immunostaining for HA (green) to detect ectopically expressed MOV10 tagged with HA, and nuclei stained with DAPI (blue). White arrow heads point to cytoplasmic foci where L1 ORF1p and HA-MOV10 co-localize. Data collected from 289 Ctrl, 275 L1UP Cl1 and 296 L1UP Cl2 mESCs (n=3 biological replicates)."
NCBI gene IDs:
"L1: 381591
MOV10: 4343"
Figure legend: "B. Scheme and representative images from colony forming assays for plasmid based retrotransposition analysis in Ctrl and L1UP mESCs comparing rate of retrotransposition upon transfection with Empty Vector (EV) or ectopic expression of HA-MOV10 (n=3 biological replicates)."
NCBI gene IDs:
"HA: 
L1: 381591
MOV10: 4343"
Figure legend: "A. Scheme of experiment and representative images showing RNP aggregate formation upon ectopic expression of full length L1 and HA-MOV10 in WT mESCs. Images are maximum intensity projections across Z stacks stained for L1 ORF1p (red) combined with immunostaining for HA (green) to detect ectopically expressed MOV10, and nuclei stained with DAPI (blue). The bottom panel shows cells where accumulation of ORF1-MOV10 was observed in relatively large foci compared to cells where co-localization of the two proteins was mostly in relatively small foci (top panel), 68-119 transfected cells were analyzed per experiment (n=3 biological replicates)."
NCBI gene IDs:
"HA: 
L1: 54596
MOV10: 4343"
Figure legend: "B. Scheme of experiment and representative images showing RNP aggregate formation upon ectopic expression of HA-ORF1 or HA-ORF1 in conjunction with T7-MOV10 in WT mESCs. Images are maximum intensity projections across Z stacks stained for HA (green) and T7 (red) to detect ectopically expressed ORF1p and MOV10 respectively, and nuclei stained with DAPI (blue). 100-147 transfected cells were analyzed per experiment (n=3 biological replicates)."
NCBI gene IDs:
"HA: 
T7: 
ORF1: 54596
MOV10: 4343"
Figure legend: "A. Representative  western blot of two matched pairs of control (Ctrl) and FBXL4  KO pool RPE1-Cas9i-mt-mKeima  cells, generated with two distinct sgRNAs. B. Quantification of data represented in A. Individual datapoints from n=3 independent colour coded experiments are shown. Error bars show standard deviation of the fold change in BNIP3  and NIX  in each KO pool normalised to their matched control pools; one-way ANOVA and Tukey's multiple comparison's test, **P&lt;0.01, ****P&lt;0.0001."
NCBI gene IDs:
"mKeima: 
Cas9: 69900935
FBXL4: 26235"
Figure legend: "D. Representative images of control or FBXL4  KO pool RPE1-Cas9i-mt-mKeima  cells. \"Magenta only\" puncta (Ex. 561) correspond to acidic mitolysosomes. Scale bar 20 µm. E. Graphs show the quantification of mitolysosomes  (mt-mkeima  \"magenta only\" puncta) per cell. A minimum of 45 cells were analysed per condition in each experiment. Individual data from two (for control 1 (Ctrl 1) and knockout 1 (KO1)) or three (control 2 (Ctrl 2) and knockout 2 (KO2)) are shown using different colours for each independent experiment. Mean and standard deviation are indicated. Bottom graph shows the individual data points. One-way ANOVA with Tukey's multiple comparison's test, *P&lt;0.05."
NCBI gene IDs:
"mKeima: 
Cas9: 69900935
FBXL4: 26235"
Figure legend: "G. Quantitative RT-PCR reactions of BNIP3  and NIX  (normalised to Actin) were performed with cDNA derived from control and FBXL4  KO RPE1-Cas9i-mt-mKeima  cells. Two primer pairs were used per target indicated by symbol shape. Three independent colour-coded experiments are shown. Error bars show standard deviation of the fold change of BNIP3  and NIX  mRNA in each KO pool normalised to their matched control pools."
NCBI gene IDs:
"mKeima: 
Actin: 60
BNIP3: 664
NIX: 665
Cas9: 69900935
FBXL4: 26235"
Figure legend: "A and B. hTERT-RPE1  cells were co-transfected with Flag  (V) or FBXL4-Flag  (FL4) and either GFP  (Vect), GFP-BNIP3  or GFP-NIX. Lysates were subjected to immunoprecipitation  (IP) with either GFP-nanobody coupled beads (A) or Flag-antibody coupled agarose  beads (B). IPs  were probed alongside the input samples for Flag  and GFP."
NCBI gene IDs:
"Flag: 
GFP: 
NIX: 
BNIP3: 664
FBXL4: 26235"
Figure legend: "D. hTERT-RPE1 cells were transfected with empty vector (V), FBXL4-Flag  (WT) or FBXL4- R482W-Flag  (RW). Lysates were subjected to IP  with Flag-antibody coupled agarose  beads and probed for SKP1  and Flag. Note that FBXL4-Flag  presents with an as yet uncharacterized higher molecular weight band (indicated by a blue arrowhead) E. Quantitation of SKP1  protein co-immunoprecipitated with FBXL4-R482W-Flag   relative to FBXL4-Flag   (WT). Data from two independent experiments are shown, normalised to the amount of SKP1  in the input and the FBXL4-Flag  in the IP."
NCBI gene IDs:
"Flag: 
FBXL4: 26235"
Figure legend: "F and G. Representative images of Control (Ctrl) and FBXL4  KO RPE1-Cas9i-mt-mKeima  cells transfected with FBXL4-Flag  wild-type (WT) or FBXL4-Flag  [R482W] for 24 hours, then fixed and stained  for either BNIP3  or NIX. Scale bar 20 µm. Quantitation of the frequency of high BNIP3 and high NIX  phenotypes observed in F and G. A minimum of 32 Flag-positive (Transfected, indicated by an asterisk in the images) and Flag-negative cells were analysed per condition in each experiment. Shown are the mean and individual datapoints of two colour-coded experiments."
NCBI gene IDs:
"Flag: 
mKeima: 
Cas9: 69900935
FBXL4: 26235"
Figure legend: "Control (Ctrl) or FBXL4  KO RPE1-Cas9i-mt-mkeima  cells were treated for 72 hours with either non-targeting (NT1), BNIP3  (B3), NIX  or HIF1α  (HIF) siRNA. A. Representative images of the mt-mKeima  reporter in Control (Ctrl2) or FBXL4  KO2 RPE1-Cas9i-mt-mkeima. \"Magenta only\" puncta (Ex. 561) correspond to acidic mitolysosomes. Scale bar: 20 µm."
NCBI gene IDs:
"mkeima: 
B3: 664
BNIP3: 664
NIX: 665
Cas9: 69900935
FBXL4: 26235
HIF: 3091
HIF1α: 3091"
Figure legend: "C. Representative western blot  of lysates prepared from  hTERT-RPE1  cells transfected with siRNA targeting BNIP3  (B3), NIX  or both for 72 hours. Cells were treated with either DMSO  or MLN4924  (1 µM) for the last 24 hours of transfection. Untransfected cells were treated alongside transfected ones with DMSO, antimycin  and oligomycin  (AO; 1 and 10 µM respectively), or MLN4924. D. Quantification of fold change in protein levels calculated from data represented in C for three colour-coded independent experiments; One way ANOVA and Dunnett's multiple comparison test *P&lt;0.05, ****P&lt;0.0001."
NCBI gene IDs:
"B3: 664
BNIP3: 664
NIX: 665"
Figure legend: "A. Representative western blot  of lysates from control or FBXL4  KO RPE1-Cas9i-mt-mKeima  cells treated with MLN4924  (1 µM) for 6 hours. B. Representative western blot of lysates from control or FBXL4  KO RPE1-Cas9i-mt-mKeima  cells pre-treated without or with MLN4924  (1 µM) for 6 hours followed by a cycloheximide  (CHX) chase (100 µg/ml)."
NCBI gene IDs:
"mKeima: 
Cas9: 69900935
FBXL4: 26235"
Figure legend: "F. TUBES-pulldown  of ubiquitylated NIX. FBXL4  KO RPE1-Cas9i-mt-mkeima  cells and matched control cells were treated as in E and lysates subjected to a TUBES pulldown."
NCBI gene IDs:
"mkeima: 
Cas9: 69900935
FBXL4: 26235"
Figure legend: "Inhibition of cell fusion by ACE2-Ig.Potent inhibition of cell fusion mediated by the SARS-CoV (left) or 2019 nCoV (right) spike (S) glycoprotein. Cells expressing diffferent S glycoprotein were incubated with indicated fusion proteins and mixed with ACE2-expressing cells. The activity of the reporter gene, β-gal, was measured as a correlate of fusion. The curves represent the best fit to the experimental data and were used for calculation of the IC50. Bars indicate the S.E."
NCBI gene IDs:
"β-gal: 
ACE2: 59272"
Figure legend: "(C) Co-immunoprecipitation using BmN4 cells transfected with the Flag-tagged DEAD-box helicases identified by shotgun proteomics. The Flag-tagged proteins and Ago3 were detected by western blotting. Full-length Flag-tagged proteins are indicated with red asterisks. h.c.: heavy chain."
NCBI gene IDs:
"Flag: "
Figure legend: "(F, G) Analysis of Siwi-piRNA and Ago3-piRNA levels in Siwi and Ago3 complexes with DDX43, respectively. The WB (western blotting) and NB (northern blotting) signals represent the PIWI protein and piRNA levels, respectively (see also Figs EV1A and EV1B). The relative ratios of piRISC were calculated based on the signal intensities of the PIWI proteins and piRNAs."
NCBI gene IDs:
"Ago3: 100125337
Siwi: 100125336"
Figure legend: "(C, D) Immunoprecipitation of endogenous Siwi, DDX43, and Vret bound with Ago3 from DDX43-depleted (C) or Vret-depleted (D) cells. The factors were detected by western blotting. Ago3 interacts with Vret and DDX43 independently of the other."
NCBI gene IDs:
"DDX43: 101735665
Vret: 101739668"
Figure legend: "(C) Immunoprecipitation of Ago3 with Flag-tagged DDX43 WT and its D399A and E400Q mutants. Ago3 and DDX43 were detected by western blotting using anti-Ago3 and anti-Flag-antibodies, respectively. Flag-EGFP was used as a negative control. The E400Q mutation in DDX43 impairs the interaction between Ago3 and DDX43. IgG h.c.: IgG heavy chain."
NCBI gene IDs:
"Flag: 
DDX43: 101735665"
Figure legend: "(F) RNA unwinding assays upon Ago3-piRISC-dependent target RNA cleavage. Unwinding activities of DDX43 WT and its D399A, E400Q, and ∆KH mutants are shown. RNAs cleaved by Ago3 are shown as \"Cleaved RNA\"."
NCBI gene IDs:
"DDX43: 101735665"
Figure legend: "B In vivo transduction profile of luciferase reporter vectors displaying variants of the enriched library capsid sequence motifs or unmodified wild type AAV2 control capsid. Four weeks after i.v. injection of 5x1010 genomic particles/mouse containing a CMV-luciferase reporter gene, luminescence was measured in the brain and control organs. Data are shown as bars (mean) with plotted individual data points (n = 3 animals/group, age 8-12 weeks)."
NCBI gene IDs:
"luciferase: "
Figure legend: "A Luciferase reporter gene vectors displaying the brain-targeted peptide NRGTEWD (\"BR1\"), unmodified wild type AAV2 capsid, or the previously reported brain-targeting peptide DSPAHPS (PPS). Vectors were intravenously injected into mice (5x1010 genomic particles/mouse). Panels show representative examples of n = 5 animals, age 8-12 weeks per group. Animals were imaged in dorsal (left panel), ventral (second from left panel), and lateral (second from right and right panel) position, 14 days after vector injection.B Close-up imaging of AAV-BR1-treated mice. Mice were imaged in dorsal and lateral position in a different color scheme allowing detailed visualization of the transduced brain in living animals, even as late as at day 264 after vector injection.C Virtual sections : sagittal, coronal, and transaxial (left panels) and three-dimensional reconstruction (right panel) of the luminescence images of a mouse injected with BR1 vector (as in A). Images were obtained by measuring different wavelengths of the emitted light (Living Image software), confirming the brain as exclusive source of luminescence."
NCBI gene IDs:
"Luciferase: "
Figure legend: "AAV-BR1 vector harboring the luciferase gene under control of the CAG promoter was administered intravenously (5x1010 genomic particles/mouse, age 8 weeks). Long-term transgene expression was analyzed by in vivo bioluminescence imaging at 16 time points during a 665-day period (n = 2 animals). Original images of analyzed animals (above) and quantification of luminescence in the brain as the region of interest = ROI (bottom)."
NCBI gene IDs:
"luciferase: "
Figure legend: "Luciferase activity and vector copy numbers were determined in tissue lysates 14 days after vector administration (5x1010 genomic particles/mouse, age 8-12 weeks).A Vector-mediated luminescence. Transgene expression was measured in AAV-BR1 harboring the luciferase gene under control of the CAG promoter and control vectors (AAV-PPS and wild type rAAV2) in brain and off-target control organs (left panel). Comparison of luminescence in the brain mediated by AAV-BR1 and control vectors (right panel), ****p &lt; 0.0001 (BR1: brain vs. all), ***p = 0.0002 (PPS: heart vs. brain/ muscle), ***p = 0.0001 (PPS: heart vs. lung/ liver/ spleen/ kidney), *p = 0.0101 (WT: heart vs. lung), *p = 0.0120 (WT: heart vs. spleen), *p = 0.0148 (WT: heart vs. kidney), *p = 0.0113 (WT: heart vs. muscle), **p = 0.0011 (Brain: BR1 vs. PPS), ***p = 0.0009 (Brain: BR1 vs. WT)."
NCBI gene IDs:
"Luciferase: 
luciferase: "
Figure legend: "Two weeks after vector injection (1.8 x1011 genomic particles/ animal), into 16-weeks old mice, Cre-mediated gene recombination driven by the CAG promoter was observed mainly in brain endothelial cells (lower panel, red). No recombination was observed in control animals without BR1-iCre-virus injection (upper panel). CD31 (green) was used as a marker for brain endothelial cells. Panels show representative examples of n = 3 animals. Scale bars represent 250 µm."
NCBI gene IDs:
"Cre: "