EMBO/SourceData huggingface dataset:
Figure legend: "Flow cytometry detection of the Bhlhe40 (grey) and Bhlhe41 (orange) transcripts by the PrimeFlow RNA assay in alveolar (right) and peritoneal (left) macrophages. A probe specific for the Cd8a mRNA (black line) was used as a negative control. Results representative of at least four independent experiments."
NCBI gene IDs:
"Cd8a: 
Bhlhe40: 20893
Bhlhe41: 79362"
Figure legend: "Frequency of WT donor (CD45.1), DKO donor (CD45.2) and recipient (CD45.1/2) cells in the indicated BM (LSK, CMP), splenic (monocytes), liver (Kupffer cells), peritoneal and alveolar macrophage populations of lethally irradiated recipients ≥ 6 weeks after transfer of a 1:1 mixture of lineage-depleted BM cells from WT and DKO mice. P values &lt; 0.05 (paired Student's t-test) between LSK and other populations are shown for frequencies of WT cells. Three mixed BM chimeras analyzed; error bars represent s.d. Representative results of three independent experiments."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "Frequency of WT donor (CD45.1), DKO donor (CD45.2) and recipient (CD45.1/2) cells among the BM LSK cells, splenic monocytes and alveolar macrophages of the recipients that were irradiated with lung shielding, treated with one dose of busulfan as described in Materials and Methods, transferred with a 1:1 mixture of lineage-depleted BM cells from WT and DKO mice and analyzed ≥ 8 weeks after transfer. Representative flow cytometry results for alveolar macrophages (left) and quantification (right) is shown."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "Surface phenotype of cells from the digested lungs (right) and quantification of donor contribution to the AM compartment (left) of AM-deficient Csf2rb-/-Csf2rb2-/-recipients (CD45.2) that were subjected to intranasal transfer of a 1:1 mixture of sorted fetal liver monocytes from WT (CD45.1/2) and DKO (CD45.2) E18.5 embryos at days 1 or 2 after birth and analyzed ≥ 7 weeks after the transfer. Cell surface phenotype of lung cells from a Csf2rb-/-Csf2rb2-/- mouse that was not subjected to the transfer is shown for comparison."
NCBI gene IDs:
"Csf2rb: 12983
Csf2rb2: 12984
CD45: 19264"
Figure legend: "WT (CD45.1) and DKO (CD45.2) AMs were sorted, mixed in a 1:1 ratio, labeled with CellTrace Violet and cultured in the presence of GM-CSF. The ratio of CD45.1 and CD45.2 cells and CellTrace Violet dilution was analyzed prior to culture (day 0) and after 7 days of culture as described in Materials and Methods."
NCBI gene IDs:
"CD45: 19264"
Figure legend: "RNA-seq analysis of Maf and Mafb expression in WT and DKO AMs, which were isolated by flow cytometry from mice in steady state (top) or from mixed BM chimeras (bottom)."
NCBI gene IDs:
"Maf: 17132
Mafb: 16658"
Figure legend: "RNA-seq analysis of Acaa1b and Hsd17b4 expression in WT and DKO AMs, which were isolated by flow cytometry from mice in steady state (top) or from mixed BM chimeras (bottom)."
NCBI gene IDs:
"Acaa1b: 235674
Hsd17b4: 15488"
Figure legend: "Presence of RNA transcripts (RNA-seq; top) at the Gas7 and Acaa1b genes in WT and DKO AMs; the ChIP-seq analysis of H3K27 acetylation in WT and DKO AMs (middle) and Bhlhe40 binding in WT AMs (bottom). All analyses performed on ex vivo AMs."
NCBI gene IDs:
"Acaa1b: 235674
Gas7: 14457"
Figure legend: "Confirmation of the expression of SnRK1K139R-YFP or SnRK1-YFP fusion protein each in two transgenic lines, SnRK1K139R-YFP-L13 and -L22 or SnRK1-YFP-L4 and -L17, by immunoblotting. The protein samples analyzed here or in (B) and (C) were all prepared from BYDV-infected barley plants at 21 dpi."
NCBI gene IDs:
"SnRK1: 
YFP: "
Figure legend: "Phosphorylation of BYDV 17K was increased in SnRK1-YFP-L4 plants overexpressing SnRK1-YFP but reduced in SnRK1K139R-YFP-L13 ectopically expressing SnRK1K139R-YFP compared with WT control. P, phosphorylated 17K bands."
NCBI gene IDs:
"SnRK1: 
YFP: "
Figure legend: "Levels of GFP siRNA detected by sRNA blotting for the panel of tobacco materials shown in (A), with arrowhead indicating the main band of GFP siRNA."
NCBI gene IDs:
"GFP: "
Figure legend: "Protein levels of PEBV CP and corrected expression of 17K, 17K5A or 17K5D in the panel of tobacco plants shown in (D), revealed using immunoblotting with anti-PEBV CP or anti-17K antibody."
NCBI gene IDs:
"17K: 1493892"
Figure legend: "17K5D, but not BYDV 17K, interacted with HvSDN1 in LC (B) assays. Expression of 17K and 17K5D in the LC assays were verified by immunoblotting using anti-17K antibody."
NCBI gene IDs:
"17K: 1493892"
Figure legend: "C 17K5D, but not 17K or 17K5A, showed binding to BYDV vsiRNA. The GST fusions of 17K, 17K5D, and 17K5A, as well as free GST, were used in the RNA EMSA assays with biotin-labeled vsiRNA899 or vsiRNA899 duplex as probes."
NCBI gene IDs:
"vsiRNA899: "
Figure legend: "Cleavage of BYDV vsiRNA899 by HvSDN1 (His-HvSDN1), with AtSDN1 (His-AtSDN1) serving as a positive control. Asterisk denotes cleavage products. The GST fusions of 17K and 17K5D did not show vsiRNA cleavage activity."
NCBI gene IDs:
"vsiRNA899: "
Figure legend: "Inhibition of HvSDN1-catalyzed vsiRNA cleavage by GST-17K5D but not GST-17K. Free GST protein served as a negative control. Asterisk denotes cleavage products."
NCBI gene IDs:
"vsiRNA: "
Figure legend: "The three mutants of 17K5D (17K5Dm4, 17K5Dm2a, and 17K5Dm2b) retained the ability to interact with HvSDN1 but their GST fusions failed to bind vsiRNA899 (D). Neither GST nor GST-17K showed vsiRNA899 binding."
NCBI gene IDs:
"vsiRNA899: "
Figure legend: "Quantitative analysis of HvSDN1 cleavage activities, with the fluorescence recorded using Typhoon FLA9500 (F and G) Compared with free GST or GST-17K, the GST fusions of 17K5D or three derivative mutants (17K5Dm4, 17K5Dm2a, and 17K5Dm2b) inhibited HvSDN1-catalzyed cleavage of fluorogenic vsiRNA899, which was revealed by a fluorospot assay (F), relative comparison of fluorescence values (G)"
NCBI gene IDs:
"vsiRNA899: "
Figure legend: "Co-IP assays revealing the presence of vsiRNA-containing HvAGO1-HvSDN1-P17K complex in BYDV-infected WT, SnRK1K139R-YFP-L13, and SnRK1-YFP-L4 plants, with mock-inoculated SnRK1-YFP-L4 individuals as controls. The immunoprecipitates, prepared using anti-HvAGO1 antibody, were analyzed for the presence of HvSDN1 (by immunoblotting with anti-HvSDN1 antibody), P17K (asterisk) and 17K (arrowhead) (using Phos-tag SDS-PAGE coupled immunoblotting), and BYDV vsiRNA (by sRNA blotting). Compared with WT control, the HvAGO1 immunoprecipitates derived from SnRK1-YFP-L4 contained higher levels of P17K and vsiRNA but less amount of HvSDN1, whereas the reverse was observed for SnRK1K139R-YFP-L13."
NCBI gene IDs:
"SnRK1: 
vsiRNA: 
YFP: "
Figure legend: "Co-IP assays demonstrating that the interaction between HvAGO1 and HvSDN1 was weakened by P17K. The immunoprecipitates were prepared using anti-HvSDN1 antibody from the panel of plants shown in (B). Relative to WT control, the HvSDN1 immunoprecipitates derived from SnRK1-YFP-L4 contained much less HvAGO1, whereas the opposite was observed for SnRK1K139R-YFP-L13."
NCBI gene IDs:
"SnRK1: 
YFP: "
Figure legend: "17K5D compromised the interaction between HvSDN1 and the PAZ domain of HvAGO1. The LUC signal resulted from the interaction between HvSDN1-HvAGO1 PAZ domain was significantly decreased in the presence of 17K5D but not that of 17K."
NCBI gene IDs:
"LUC: 
17K: 1493892"
Figure legend: "Verification of 17K5D expression in the transgenic lines 17K5D-L3 and -L7 by immunoblotting with anti-17K antibody."
NCBI gene IDs:
"17K: 1493892"
Figure legend: "BYDV vsiRNA abundance in the set of samples shown in (B), as detected by sRNA blotting at 14 dpi."
NCBI gene IDs:
"vsiRNA: "
Figure legend: "Relative to TaSDN1-DD line, TaSDN1 protein was decreased in TaSDN1-Dd and TaSDN1-dd mutants, with a stronger reduction found for TaSDN1-dd. The reactive TaSDN1 protein detected in TaSDN1-dd was caused by the un-mutated TaSDN1-A and -B homoeologs."
NCBI gene IDs:
"SDN1: "
Figure legend: "protein (M) levels of BYDV CP gene in TaSDN1-DD, TaSDN1-Dd and TaSDN1-dd plants at 21 dpi. "
NCBI gene IDs:
"SDN1: "
Figure legend: "BYDV vsiRNA abundance in TaSDN1-DD, TaSDN1-Dd, and TaSDN1-dd plants detected by sRNA blotting at 21 dpi of BYDV."
NCBI gene IDs:
"SDN1: 
vsiRNA: "
Figure legend: "In vitro uptake is independent of components involved in homotypic vacuolar fusion. (A) Standard uptake reactions were performed (60 min, 27°C) in the presence of the indicated reagents using a mixture of vacuoles from strains DBY5734-16 (Δpep4) and DBY5734-19 (Δpho8). Therefore, homotypic vacuolar fusion and luciferase uptake could be assayed simultaneously from the same sample. Four independent experiments were averaged. Error bars indicate SD. For averaging, uptake and fusion activities of the control sample without inhibitor were set at 100%. Inhibitors were used at the following concentrations: anti-Sec17p, 1 μM; anti-Sec18p, 1 μM; Gdi1p, 2.5 μM; anti-Vam3p, 1 μM; anti-Vam7p, 1 μM; and anti-Nyv1p, 1 μM."
NCBI gene IDs:
"pep4: 855949
pho8: 852092"
Figure legend: "(B) Vacuoles and cytosols were prepared from sec18-1 and from corresponding wild-type (Wt) cells grown and spheroplasted at 23°C. The isolated vacuoles and the cytosols were exposed to heat treatment (10 min, 37°C). Standard uptake reactions were performed with vacuoles and cytosols in the indicated combinations. Control reactions were performed in the absence of ATP. Five independent experiments were averaged; the sample with vacuoles and cytosol from wild-type cells was used as the 100% reference. Error bars indicate SD. Mut, mutant."
NCBI gene IDs:
"sec18: 852372"
Figure legend: "(B  3rd instar larval fat bodies  were  stained  by BODIPY (green) for  lipid droplets  and  DAPI (blue) for  nuclei. Scale bars represent 25 µm. (B) RDH/CG2064 RNAi suppresses the enlarged  lipid droplet   phenotype in CSN2  RNAi under  DGAT1  overexpression (ppl&gt;DGAT1) or high-fat diet conditions. (C) Quantification of the   lipid droplet  size in (B). HFD: high-fat diet. data were analyzed by one-way ANOVA with a post hoc Turkey's multiple-comparison test. Each point represents data from one fat body, and at least 30 cells were examined in each  fat body. Error bars represent ± SEM. ***: P &lt; 0.001; **: P &lt; 0.01; NS: non-significant."
NCBI gene IDs:
"CSN2: 34225
CG2064: 35708
DGAT1: 44887
ppl: 40349"
Figure legend: ", F 3rd instar larval fat bodies were stained  by  BODIPY  (green) for   lipid droplets and DAPI (blue) for  nuclei. Scale bars represent 25 µm. (F) RDH/CG2064 overexpression increases  lipid droplet  size in ppl&gt;DGAT1 larvae. (G) Quantification of the  lipid droplet size in (F). data were analyzed by one-way ANOVA with a post hoc Turkey's multiple-comparison test. Each point represents data from one fat body, and at least 30 cells were examined in each fat body. Error bars represent ± SEM. ***: P &lt; 0.001; **: P &lt; 0.01; NS: non-significant."
NCBI gene IDs:
"CG2064: 35708
DGAT1: 44887
ppl: 40349"
Figure legend: "3rd instar larval fat bodies were stained by BODIPY (green) for  lipid droplets   and DAPI  (blue) for nuclei. Scale bars represent 25 µm. (H) Relative TAG  levels in different genetic backgrounds were measured by glyceride assay  kit. For quantification, TAG levels were normalized to protein. (I) RDH/CG2064 and  CG2065 deletion does not affect lipid droplet   size under normal or high-fat diet conditions. data were analyzed by one-way ANOVA with a post hoc Turkey's multiple-comparison test. Each point represents data from one fat body, and at least 30 cells were examined in each fat body. Error bars represent ± SEM. ***: P &lt; 0.001; **: P &lt; 0.01; NS: non-significant."
NCBI gene IDs:
"CG2064: 35708
CG2065: 35707"
Figure legend: "(B) 3rd instar larval fat bodies  were  stained  by BODIPY (green) for lipid droplets and DAPI  (blue) for  nuclei. Overexpressing wild-type or site-mutated RDH/CG2064 increases the lipid droplet size in larvae with DGAT1  overexpression (ppl&gt;DGAT1). Scale bar represents 25 µm."
NCBI gene IDs:
"CG2064: 35708
DGAT1: 44887
ppl: 40349"
Figure legend: "(E) Lysates of Drosophila S2  cells expressing full-length Plin1 or  Plin1 with a C-terminal deletion (Plin1ΔC) were  immunoprecipitated with anti-Flag antibody and then probed by anti-GFP or anti-Flag antibody in a western blot. Plin1ΔC coimmunoprecipitated with  RDH/CG2064."
NCBI gene IDs:
"Plin1: 42810"
Figure legend: "(F) 3rd instar larval fat bodies  were  stained by BODIPY  (green) for lipid droplets  and  DAPI (blue) for nuclei. Under  high-fat conditions, RDH/CG2064 overexpression increases the lipid droplet size, and Plin2 RNAi suppresses the enlargement induced by  RDH/CG2064 overexpression. Scale bar represents 25 µm."
NCBI gene IDs:
"CG2064: 35708
Plin2: 32437"
Figure legend: "(A, B Images of  Plin2-GFP in 3rd instar larval fat body  cells  of different genotypes. Scale bar represents 25 µm. (A) CSN2 RNAi or RDH/CG2064 overexpression reduces the peripheral localization of  Plin2-GFP and increases the localization  of Plin2-GFP on small peri-nuclear lipid droplets (boxed).  DAPI (blue)  stains nuclei. White arrows indicate peripheral lipid droplet. Red arrows indicate medial lipid droplets. (B) The peripheral localization  of Plin2-GFP is greatly reduced in  fat body  cells from  larvae with  CSN2 RNAi or RDH/CG2064 overexpression. Images were taken by focusing on the periphery of fat cells."
NCBI gene IDs:
"CSN2: 34225
CG2064: 35708"
Figure legend: "C, Images of  Plin2-GFP in  3rd instar larval fat body cells of different genotypes. Scale bar represents 25 µm. (C) In fat body cells from ppl&gt;DGAT1 larvae with CSN2 RNAi or  RDH/CG2064 overexpression, the  localization of Plin2-GFP  is greatly reduced at the periphery  and is dramatically increased on large  medial lipid droplets. White arrows indicate peripheral lipid droplet. Red arrows indicate medial lipid droplets. (D) Fluorescence intensity line plots of the images in (C). GFP intensity along the line across a lipid droplet was measured by ImageJ. In ppl&gt;DGAT1 larvae with  CSN2 RNAi or RDH/CG2064 overexpression, Plin2-GFP localization on large  medial lipid droplets  is represented by two peaks. More than 30 lipid droplets were measured for each genotype. The plot shown is a typical image curve for the indicated genotype."
NCBI gene IDs:
"CSN2: 34225
CG2064: 35708
DGAT1: 44887
ppl: 40349"
Figure legend: "(G) The  peripheral lipid droplets in larval fat body cells  from different genetic backgrounds were  stained by BODIPY (green). CSN3 RNAi or RDH/CG2064 overexpression reduces the number of peripheral lipid droplets in both wild-type and ppl&gt;DGAT1 larvae."
NCBI gene IDs:
"CG2064: 35708
CSN3: 40308
DGAT1: 44887
ppl: 40349"
Figure legend: "(A, Confocal  images of Bmm-GFP (green) in 3rd instar larval fat bodies of different genotypes. DAPI (blue) stains nuclei. Scale bar represents 25 µm. (A) Knocking down  CSN2 or overexpressing  RDH/CG2064  in animals with DGAT1 overexpression (ppl&gt;DGAT1) decreases the level and  lipid droplet localization  of Bmm-GFP. (B) The GFP intensity along the line across a  lipid droplet  in (A) was measured by ImageJ. The  lipid droplet localization  of Bmm-GFP, represented by two peaks, is clearly visible in fat cells from ppl&gt;DGAT1 larvae, but it is lost in  fat cells from ppl&gt;DGAT1 larvae  with CSN2  RNAi or overexpression of RDH/CG2064. More than 30  lipid droplets of each genotype were measured. One typical image curve is shown for each genotype."
NCBI gene IDs:
"CSN2: 34225
CG2064: 35708
DGAT1: 44887
ppl: 40349"
Figure legend: "F)  Confocal  images of  Bmm-GFP  (green) in 3rd instar larval fat bodies of different genotypes. DAPI  (blue)  stains nuclei. Scale bar represents 25 µm. (F) Overexpression of Plin2 decreases the level and  lipid droplet localization of Bmm-GFP in  fat cells  from  larvae  with DGAT1  overexpression. (G) GFP intensity along the line across a  lipid droplet  from (F) was measured by ImageJ. The  lipid droplet localization  of Bmm-GFP, represented by two peaks, was evident in fat cells from ppl&gt;DGAT1 larvae, but is lost in ppl&gt;DGAT1 larvae with overexpression of  Plin2. More than 30 lipid droplets of each genotype were measured. One typical image curve is shown for each genotype."
NCBI gene IDs:
"Plin2: 32437
DGAT1: 44887
ppl: 40349"
Figure legend: "(H) 3rd instar larval fat bodies were stained  by BODIPY (green) for   lipid droplets and  DAPI (blue) for nuclei. bmm RNAi increases the lipid droplet size in fat cells  from  larvae with  DGAT1 overexpression. Scale bar represents 25 µm."
NCBI gene IDs:
"bmm: 39611
DGAT1: 44887"
Figure legend: "(A) S2  cells expressing  RDH/CH2064-GFP  were treated with the protein synthesis inhibitor cycloheximide  (CHX) for the indicated time (h: hours). The level of RDH/CH2064-GFP was determined by  western blotting using anti-GFP antibody. Tubulin was used as loading control. (B) Quantification of the relative RDH/CH2064-GFP protein levels in (A). Error bars represent ± SEM."
NCBI gene IDs:
"GFP: 
CH2064: 35708"
Figure legend: "(C) S2 cells expressing Bmm-GFP were treated with the protein synthesis inhibitor cycloheximide (CHX) for the indicated time (h: hours). The level of Bmm-GFP was determined by western blotting  using anti-GFP antibody. Tubulin was used as loading control. (D) Quantification of the relative Bmm-GFP protein levels in (C). Error bars represent ± SEM."
NCBI gene IDs:
"GFP: 
Bmm: 39611"
Figure legend: "(B) RT-PCR analysis of the expression of NKG2D in γδ T cells isolated from indicated samples."
NCBI gene IDs:
"NKG2D: 22914"
Figure legend: "(C) RT-PCR analysis of the expression of IFN-γ and TNF-α in γδ T cells isolated from the peripheral blood of healthy controls and metastatic cancer patients. (D) RT-PCR analysis of the expression of miR-125b-5p and miR-99a-5p in γδ T cells isolated from indicated samples. (E) RT-PCR analysis of the expression of miR-181a(-5p and -2-3p) in γδ T cells isolated from indicated samples."
NCBI gene IDs:
"IFN-γ: 3458
miR-125b-5p: 406911
miR-181a: 406954
miR-99a-5p: 407055
TNF-α: 7124"
Figure legend: "(A) RT-PCR analysis of the expression of miR-181a(-5p and -2-3p) in γδ T cells isolated from thymic biopsies, cultured for 11 days with IL-7 or IL-2 (n=12-15 independent biological samples). (B) RT-PCR analysis of the expression of miR-181a(-5p and -2-3p) in γδ T cells freshly isolated from thymic biopsies (Thymus) and PBLs (n=6-14 independent biological samples)."
NCBI gene IDs:
"miR-181a: 406954"
Figure legend: "(C-D) RT-PCR analysis of the expression of miR-181a(-5p and -2-3p) in γδ T cells isolated from thymus (C) or from PBLs (D) and cultured with the indicated cytokines, respectively for 11 days (C) or for 4-6 days (D). When indicated, γδ PBLs were also co-cultured with an anti-TGF-β blocking antibody. Data are normalized to the value obtained in IL-7 cultures (n=4-10 independent biological samples)."
NCBI gene IDs:
"miR-181a: 406954"
Figure legend: "(E-F) RT-PCR analysis of the expression of miR-181a(-5p and -2-3p) versus FACS analysis of the expression of TNF-α (left panel), IFN-γ (middle panel) and NKGD2 (right panel) in γδ T cells isolated from thymus (E) and PBLs (F). Samples were either freshly isolated or cultured for 11 days (γδ thymocytes) or for 6 days (γδ PBLs) with the indicated cytokines (n=5 independent biological samples)."
NCBI gene IDs:
"miR-181a: 406954"
Figure legend: "(D) FACS analysis of the expression of Annexin V in miR-181a versus empty transduced (GFP+) and untransduced (GFP-) γδ thymocytes, cultured with IL-7 plus IL-2 for 11 days (n=5 independent biological samples)."
NCBI gene IDs:
"GFP: 
miR-181a: 406954"
Figure legend: "(E) RT-PCR analysis of the expression of Bcl2 and Bcl-xL (normalized to the values obtained with the empty virus) in transduced (GFP+) γδ thymocytes, cultured with IL-7 plus IL-2 for 11 days (n=5 independent biological samples)."
NCBI gene IDs:
"GFP: 
Bcl-xL: 572
Bcl2: 596"
Figure legend: "(F) FACS-derived expression of indicated surface and intracellular markers in miR-181a versus empty transduced (GFP+) and untransduced (GFP-) γδ T thymocytes, cultured with IL-7 plus IL-2 for 11 days (n=7-11 independent biological samples)."
NCBI gene IDs:
"GFP: 
miR-181a: 406954"
Figure legend: "(B) RT-PCR analysis of the expression of the indicated genes associated with Mapk, Notch, Irf4 or Stat1 signaling in sorted GFP+ γδ thymocytes transduced with empty or miR-181a viral vector. Data are normalized to the value obtained with the empty virus. Samples were cultured with IL-7 plus IL-2 for 11 days (n=3-5 independent biological samples)."
NCBI gene IDs:
"GFP: 
Irf4: 3662
Mapk: 10746
miR-181a: 406954
Notch: 4853
Stat1: 6772"
Figure legend: "(C) Dual luciferase reporter assay performed to verify binding between miR-181a and Map3k2, Notch2, Irf4 or Stat1 mRNA. HEK293 T cells were co-transfected with a pmirGLO Dual-Luciferase miRNA Target Expression Vector containing either the WT or mutated (Mut) 3′ UTR target sites plus miR-181a. A negative construct (without miR-181a binding sites) was included. Firefly/Renilla ratios were normalized to those obtained for the empty vector (n=4 independent biological samples)."
NCBI gene IDs:
"Luciferase: 
Irf4: 3662
Map3k2: 10746
miR-181a: 406954
Notch2: 4853
Stat1: 6772"
Figure legend: "(E-G) RT-PCR analysis in circulating γδ T cells of Map3k2 or Notch2 expression (E), Nkg2d or Tnfa expression (F), and NKG2D or TNF-α protein expression (G) after siRNA-mediated knockdown of Map3k2 or Notch2. In each experiment, a control condition was also used where a nontargeting negative control siRNA was transfected. γδ were cultured with IL-7 for 4 days (n=6 independent biological samples)."
NCBI gene IDs:
"Nkg2d: 22914
Map3k2: 10746
Notch2: 4853
Tnfa: 7124"
Figure legend: "HEK293 cells were transfected with α11.2, α2δ-1, and β2A and cultured for 22-24h before cell attached patch recording in 110 mM Ba2+. Single CaV1.2 channel recordings of CaV1.2 WT and K1647A. Holding potential (HP) was -80 mV and test potential (TP) 0 mV. Shown are 10 consecutive sweeps from representative experiments (see Fig. EV 4 for more sweeps). Dot plots and means + SEM for availability (i.e., likelihood that a sweep had at least one event; c), NPo (d), Po (e), unitary current amplitude i (e), and the mean of the current of a single channel at any point in time calculated from the ensemble averages of each experiment (g) (**p&lt;0.01, ***p&lt;0.001 compared to WT; one-way ANOVA with Bonferroni post-hoc test (c) or Welch ANOVA with Tamhane T2 test (d-g); n = 7 to 35 see Table 5 for more details)."
NCBI gene IDs:
"α11.2: 24239
α2δ-1: 100009105
β2A: 116600"
Figure legend: "HEK293 cells were transfected with α11.2, α2δ-1, and β2A plus, if indicated, α-actinin-1 before cell attached patch recording. Representative single CaV1.2 channel recordings of WT CaV1.2 alone or with WT or E847K/E851K mutant α-actinin-1. Mean assemble averages for all experiments for each combination. Dot plots and means + SEM for availability (c), NPo (d), Po (e), unitary current amplitude i (f), and the mean of the current of a single channel calculated from the ensemble averages of each experiment (g) (*p&lt;0.05, **p&lt;0.01, ****p&lt;0.001; unpaired t-test; n = 13 to 35; see Table 6 for more details)."
NCBI gene IDs:
"α-actinin-1: 87
α11.2: 24239
α2δ-1: 100009105
β2A: 116600"
Figure legend: "HEK293 cells were transfected with α11.2 with the K1647E mutation, α2δ-1, and β2A plus α-actinin-1 WT or E847K/E851K before cell attached patch recording. Representative single CaV1.2 channel recordings of CaV1.2 K1647E with WT or E847K/E851K α-actinin-1. Mean assemble averages for all experiments for both combinations. Dot plots and means + SEM for availability (c), NPo (d), Po (e), unitary current amplitude i (f), and mean of the current of a single channel calculated from the ensemble averages of each experiment (g) (**p&lt;0.01; unpaired t-test; n = 16 to 30; see Suppl. Fig. 8 and Table 6 for more details)."
NCBI gene IDs:
"α-actinin-1: 87
α11.2: 24239
α2δ-1: 100009105
β2A: 116600"