EMBO/SourceData huggingface dataset:
Figure legend: "E Immunoblot of cellular ubiquitination assay showing re-expression of FBXW7 in knockout cells leads to increased CCNL1 ubiquitination. Representative of three independent replicates."
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "B Immunoblot of HPAF-II wild-type and FBXW7-/- released from overnight nocodazole treatment at indicated time points,"
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "D Representative images of GFP-tubulin labeled wild-type, FBXW7-/-, and CCNL1OE HPAF-II cells monitoring cellular progression through cytokinesis following arrest in prometaphase using monastrol (150μM for 18 hours). E Quantification of cytokinetic timing, n=20, 25, 26 (wild-type, FBXW7-/, CCNL1OE ) pooled from three independent experiments F Immunoblot of wild-type, FBXW7-/- and CCNL1OE HPAF-II cell lines demonstrating varying levels of CCNL1."
NCBI gene IDs:
"CCNL1: 57018
FBXW7: 55294"
Figure legend: "A Clonogenic growth assays for HPAF-II wild-type and FBXW7-/- polyclonal, and FBXW7-/- clones in presence of various OTS964 doses for 14 days. Representative images of 4 independent replicates, quantified by crystal violet absorbance at A595 and B Clonogenic growth assays for HPAF-II wild-type and CCNL1OE polyclonal, and CCNL1OE clones in presence of various doses of OTS964 for 14 days. Representative images of 4 independent replicates, quantified by crystal violet absorbance at A595"
NCBI gene IDs:
"CCNL1: 57018
FBXW7: 55294"
Figure legend: "C Immunoblot for the indicated proteins from lysates extracted from the individual FBXW7-/- and CCNL1OE clones."
NCBI gene IDs:
"CCNL1: 57018
FBXW7: 55294"
Figure legend: "B Immunoblot of lysates from cervical cell lines demonstrating C33A cells (FBXW7R465H) express high levels of CCNL1, representative of three independent replicates."
NCBI gene IDs:
"FBXW7: 55294"
Figure legend: "B Average seizure score of KA-treated WT and Fxr2 KO mice over time from t=0 (injection time) to 2 h. * p &lt; 0.05 compared to WT mice (two-way Repeated Measures ANOVA; interaction effect of time and genotype; F1,666 = 10.05, p = 0.0016). KA-treated WT mice (n=16), KA-treated Fxr2 KO mice (n=12). Data are presented as mean ± Standard Error of the Mean (SEM). D Average seizure score of pilocarpine-treated WT and Fxr2 KO mice over time from t=0 (injection time) to 2 h (two-way Repeated Measures ANOVA). Pilocarpine-treated WT mice (n=6), pilocarpine-treated Fxr2 KO mice (n=6). Data are presented as mean ± SEM."
NCBI gene IDs:
"Fxr2: 23879"
Figure legend: "B PSD-95 mRNA had lower read counts in Fxr2 KO samples (n=3) compared to WT samples (n=3) (+ adjusted p &lt; 0.1, two-tailed Student's t-test). Data are presented as mean ± SEM."
NCBI gene IDs:
"PSD-95: 13385
Fxr2: 23879"
Figure legend: "A Representative Western blot and quantification of GluK5, PSD-95, GluN2B and mGluR5 proteins. Endogenous GluK5, PSD-95, GluN2B and mGluR5 in Fxr2 KO mice (n=9) compared to WT mice (n=11). * p &lt; 0.05, ** p &lt; 0.01, significant difference between WT and Fxr2 KO (two-tailed Student's t-test: GluK5: t18 = 0.87, p = 0.394; PSD-95: t18 = 2.29, p &lt; 0.05; GluN2B: t18 = 3.09, p &lt; 0.01; mGluR5: t18 = 3.74, p &lt; 0.01). Quantified bands are highlighted with an asterisk or squared bracket. Data are presented as mean ± SEM."
NCBI gene IDs:
"Fxr2: 23879"
Figure legend: "A Bar plot showing phospho-ERK1/2 over total ERK1/2 levels in vehicle- and KA-treated WT (closed and open black circles, vehicle-treated WT n=6; KA-treated WT n=6) and Fxr2 KO mice (closed and open magenta circles, vehicle-treated Fxr2 KO n=5; KA-treated Fxr2 KO n=6). *** p &lt; 0.001 comparison between vehicle- and KA-treated WT (two-way ANOVA: interaction effect between genotype and treatment: F1,19 = 24.66, p &lt; 0.001). Data are presented as mean ± SEM. B Phospho-ERK1/2 levels in vehicle- and pilocarpine-treated WT (n=4-6) and Fxr2 KO mice (n=4-6). Both genotypes responded with long-lasting seizures upon pilocarpine administration * p &lt; 0.05 (two-way ANOVA: main effect for treatment: F1,16 = 10.98, p &lt; 0.01). Data are presented as mean ± SEM. "
NCBI gene IDs:
"Fxr2: 23879"
Figure legend: "D Effect of forced SAC  activation. Oocytes  expressing MAD1-mNeonGreen-CENP-C (green), together with major satellite-mClover  (green) and H2B-mCherry (red), were imaged. Time  after the start of imaging   (h:mm) is shown. Oocytes were categorized based on anaphase  figures (n = 24, 23 oocytes)."
NCBI gene IDs:
"mNeonGreen: 
CENP-C: 12617
MAD1: 17120"
Figure legend: "D. Subcellular localization  of BES1-CFP in  roots  of 7-day-old plants after  heat shock. 35S:BES1-CFP seedlings were grown at 21°C and either left untreated or exposed for 60 minutes to 45°C. Left: representative photos. Right: quantification of the nuclear/cytoplasmic signal. Data show the mean ±SD. n=25. The asterisks indicate significant differences to the untreated plants by Student's t-test (***P≤0.001)."
NCBI gene IDs:
"CFP: 
BES1: 838518"
Figure legend: "F. Heat stress resistance of bes1-1, bes1-2 and  bes1-D as compared to wild-type. For basal resistance assays (top charts) plants were directly exposed to 45°C for the indicated  time. For acquired resistance (bottom charts), plants  were primed with the respective temperatures for 120 minutes before transfer to 45°C for 150 minutes. Data show the mean ±SE. n=7. The asterisks indicate significant differences compared to wild-type by Student's t-test (*P≤0.05; **P≤0.01; ***P≤0.001)."
NCBI gene IDs:
"bes1: 838518"
Figure legend: "B.  Immunodetection of BES1-CFP from heat-stressed plants, treated with epiBL or BRZ. 35S:BES1-CFP plants were treated for 24 hours with  DMSO (control), 1 μM 24-epiBL or 5 μM  BRZ  and then heat-stressed as follows: lane 1: untreated control; lane 2: 45°C for 30 minutes; lane 3: 45°C for 60 minutes; lane 4: 45°C for 60 minutes and recovery at 21°C for 180 minutes."
NCBI gene IDs:
"CFP: 
BES1: 838518"
Figure legend: "C, D.  Immunodetection  of BES1-CFP  from heat-treated bri1-1 or det2-1 plants. Left: phenotypes of 10-day-old  plants of all lines as compared to wild-type  Col-0 (wt). Right: Immunoblots  from the lines grown and treated as in B."
NCBI gene IDs:
"bri1: 830095
det2: 818383"
Figure legend: "F. Immunodetection  of  BES1-CFP  from  heat-treated  bin2-1 plants."
NCBI gene IDs:
"bin2: 827605"
Figure legend: "A.  Immunodetection of BES1-CFP from  heat-stressed plants, treated with ABA or Fluridone (Flu). 35S:BES1-CFP plants were treated for 24 hours with DMSO (control), 5 μM ABA or 10 μM  Fluridone and heat-stressed  as follows: lane 1: untreated control; lane 2: 45°C for 30 minutes; lane 3: 45°C for 60 minutes. The non-phosphorylated BES1 bands were quantified with ImageJ and the obtained values are shown in blue."
NCBI gene IDs:
"CFP: 
BES1: 838518"
Figure legend: "C. Immunodetection of native  BES1 from heat-stressed and epiBL-treated abitM plants. abitM and wild-type plants were grown on ½ MS medium either containing  DMSO as a control or 1 μM epiBL and either left untreated or exposed to 45°C for 60 minutes. The non-phosphorylated BES1 bands were quantified with ImageJ and the obtained values are shown in blue."
NCBI gene IDs:
"abi: 828714"
Figure legend: "C. ChIP to determine BES1-CFP enrichment on the promoters of HSP70.3, HSP70.4  and HSP90.1 following heat stress. 35S:BES1-CFP and wild-type  plants grown at 21°C were either left untreated or treated with 45°C for 60 minutes. BES1-CFP  was immuno-precipitated  with α-GFP beads. Fragments were quantified in the precipitates with  qPCR and the ratios of samples with antibody to without antibody were calculated. TA3 (70.3p and 90.1p) or 5s rRNA (70.4p) were used for normalization. UBQ5 served as an internal control. Data show the mean ±SE. n=3 biological replicates measured in 3 technical repeats. Significant differences as compared to wild-type at 21°C, calculated by Student's t-test, are shown (*P≤0.05; **P≤0.01; ***P≤0.001)."
NCBI gene IDs:
"5s rRNA: 
CFP: 
TA3: 
HSP70.3: 820102
BES1: 838518
HSP70.4: 820438
HSP90.1: 835341
UBQ5: 825398"
Figure legend: "A. EMSAs showing in vitro DNA  interaction of recombinant  BES1  and/or  HSFA1 with HSE-containing fragments of the  HSP70.3  and  HSP90.1 promoters. Unlabeled competitor fragments with functional  HSEs (C1 +HSE) or mutated versions (C2 -HSE) were used for competition studies in 100x molar access (lanes 6 and 7)."
NCBI gene IDs:
"HSP70.3: 820102
HSP90.1: 835341"
Figure legend: "C.  LUC assays in protoplasts  of wild-type, bes1-D or the hsfA1qM plants. LUC  reporter constructs, driven by 2 kb of the HSP70.3, HSP70.4 and HSP90.1 promoters, were tested either alone (0, with empty effector plasmid) or with  BES1 or HSFA1a  as effectors and LUC  transactivation was analyzed. Data show the mean ±SD. n=5. Statistically significant difference at P≤0.05 of results is indicated with different letters and was determined by Student's t-test."
NCBI gene IDs:
"LUC: 
HSP70.3: 820102
bes1: 838518
BES1: 838518
hsfA1: 827496
HSFA1a: 827496
HSP70.4: 820438
HSP90.1: 835341"
Figure legend: "A, B. Constitutive and BR-responsive  growth  of bes1-DxhsfA1qM seedlings. Hypocotyl  elongation of 7-day-old dark-grown (A) and light-grown (B)  seedlings on medium containing either 1 μM  epi-BL, 1 μM  BRZ or DMSO as a control (c). Left: Mean and SD of 30 plants. Significant difference at P≤0.05 of results is indicated with different letters and was determined with a Student's t-test. Right: photos of representative plants."
NCBI gene IDs:
"bes1: 838518
hsfA1: 827496"
Figure legend: "C. Expression of BR-regulated genes in bes1-DxhsfA1qM, as compared to its parents and respective wild-types analyzed by  qPCRs. Data show the mean ±SD. n=3 biological repeats, each measured in 3 technical replicates, normalized to  UBC. Statistically significant difference at P≤0.05 of results is indicated with different letters and was determined by Student's t-test."
NCBI gene IDs:
"bes1: 838518
hsfA1: 827496"
Figure legend: "B Representative images of Drosophila adult wings that resulted from larvae wing imaginal discs expressing a control RNAi (mCherry RNAi), a lds RNAi, or co-expressing san RNAi with control RNAi (san RNAi control RNAi) or with lds RNAi (san RNAi lds RNAi)."
NCBI gene IDs:
"mCherry: 
lds: 45894
san: 44724"
Figure legend: "A Representative images of cells in the wing disc pouch upon nub-Gal4-mediated RNAi for lds (bottom) compared to a nub-Gal4 control (top). Cells also express HisH2AvD-mRFP1 (magenta) and centromeric marker cid-EGFP (green); Times (min:sec) are relative to NEBD; scale bars 5 µm and applies to all images. B Mitotic timing defined from NEBD to anaphase onset; each dot represents a different cell (n=77 cells from at least 6 independent movies per condition); black lines represent mean; statistical analysis was performed using the non-parametric Mann-Whitney test. Data information: Scale bars are 10 µm and apply to all images."
NCBI gene IDs:
"Gal4: 855828
lds: 45894
nub: 34669"
Figure legend: "A Analysis of ongoing transcription monitored by MCP-EGFP labelled nascent transcripts on a reporter containing MS2 loops (green), in the referred experimental conditions. Flies also express H2AvD-mRFP1 (magenta). Scale bar is 10 µm and applies to all images."
NCBI gene IDs:
"EGFP: 
MCP: 
mRFP1: 
MS2: 
H2AvD: 43229"
Figure legend: "B Segregation defects upon inhibition of transcription in luciferase and lds RNAi. Graph depicts mean ± s.e.m. of the frequencies of defects scored in 11 (luciferase RNAi, H2O and lds RNAi, α-amanitin) or 8 (lds RNAi, H2O and Luc RNAi, α-amanitin) independent embryos; a total of ~800 nuclei were scored per condition."
NCBI gene IDs:
"Luc: 
luciferase: 
lds: 45894"
Figure legend: "A Wing morphology phenotype obtained upon depletion of Lds combined with control co-depletion (luciferase RNAi) or expression/depletion of chromosome architecture modulators. Wing morphology defects were classified according to their severity (1= normal wings and 5= no wing); depicted numbers indicate the average morphology grade and the number of flies counted, from at least 2 independent experiments."
NCBI gene IDs:
"luciferase: 
Lds: 45894"
Figure legend: "B Live cell imaging analysis of the kinetics of Rad21-EGFP (green) removal from mitotic chromosomes in luciferase- and lds-depleted embryos. DNA is marked with H2AvD-mRFP1 (magenta); times are relative to NEBD; scale bar is 10 µm and applies to all images."
NCBI gene IDs:
"luciferase: 
lds: 45894"
Figure legend: "(A, Chromatin localisation of chromosome assembly factors (Top2 (A) in green) upon RNAi for luciferase (left) or lds (right) in early embryos. DNA is marked with H2AvD-mRFP1 (magenta); scale bars are 10 µm and apply to all images."
NCBI gene IDs:
"luciferase: 
lds: 45894"
Figure legend: "C) Chromatin localisation of Condensin I subunit Barren (C), in green) upon RNAi for luciferase (left) or lds (right) in early embryos. DNA is marked with H2AvD-mRFP1 (magenta); scale bars are 10 µm and apply to all images."
NCBI gene IDs:
"luciferase: 
lds: 45894"
Figure legend: "G Telophase images of embryos depleted for luciferase (left), Cap-D2 (middle) and wapl (right) RNAi, and expressing Lds-EGFP (green) and HisH2AvDmRFP1 (magenta). Bottom panels depict Lds-EGFP alone. Scale bar is 10 µm and applies to all images."
NCBI gene IDs:
"luciferase: 
Cap-D2: 43491
wapl: 31187"
Figure legend: "A, Representative images of mitotic nuclei in prophase (1 min before NEBD) and metaphase (1 min before anaphase onset), upon RNAi for luciferase/lds, microinjected with water (A) Scale bars are 5 µm and refer to all images."
NCBI gene IDs:
"luciferase: 
lds: 45894"
Figure legend: "B Representative images of mitotic nuclei in prophase (1 min before NEBD) and metaphase (1 min before anaphase onset), upon RNAi for luciferase/lds ICRF-193 (B). Scale bars are 5 µm and refer to all images."
NCBI gene IDs:
"luciferase: 
lds: 45894"
Figure legend: "(B) Representative kymographs and quantification of anterograde, retrograde, mean velocity, and linear flow rate of BDNF-mCherry-containing vesicles in cortical neurons transfected with siMecp2 (n= 753 vesicles/ 81 axons) or siControl (siCtl) (n= 894 vesicles/ 94 axons), inducing significant silencing of Mecp2 (3 independent experiments; unpaired t-test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001 Data are presented as the means ± SEM."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "(C) Quantification of anterograde, retrograde, mean velocity, and linear flow rate of BDNF-mCherry trafficking into cortical neurons obtained from WT (n= 753 vesicles/ 81 axons), HTTSA (n= 812 vesicles/ 81 axons), or HTTSD (n= 787 vesicles/ 82 axons) homozygous knock-in mice in which S421 of HTT was replaced by an alanine (HTTS421A/S421A or HTTSA), mimicking the absence of phosphorylation, or by an aspartic acid (HTTS421D/S421D or HTTSD), mimicking constitutive phosphorylation (3 independent experiments; one-way ANOVA test with Tukey's multiple comparisons). Data information: , ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant. Data are presented as the means ± SEM."
NCBI gene IDs:
"HTT: 15194"
Figure legend: "(D) Cell viability in WT (n=3), HTTSA (n=1), or HTTSD (n=2) neurons measured by MTT assay. No differences were observed between the different groups (3 independent experiments; one-way ANOVA Kruskal-Wallis test, p=0.5701 Data information: ns = not significant. Data are presented as the means ± SEM."
NCBI gene IDs:
"HTT: 15194"
Figure legend: "(E) Kymographs and quantification of anterograde, retrograde, mean velocity, and linear flow rate of BDNF-mCherry axonal trafficking into WT (n= 894 vesicles/ 94 axons), HTTSA (n= 812 vesicles/ 82 axons), or HTTSD (n=787 vesicles/ 94 axons) cortical neurons transfected with siCtl or siMecp2, which significantly silenced Mecp2 (3 independent experiments; one-way ANOVA with Tukey's multiple comparisons). (F) Cell viability in WT, HTTSA, or HTTSD neurons transfected with siCtl or siMecp2 measured by MTT assay (WT siCtl: n=3; WT siMecp2: n=3; HTTSD siMecp2: n=3; HTTSA siMecp2: n=2). No differences were observed between the different groups (3 independent experiments; one-way ANOVA Kruskal-Wallis test, p=0.5701) Data information: Scale bars = 20 µm. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant. Data are presented as the means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "(A) Left: The ratio between the striatal and the cortical BDNF determined by quantifying the level of BDNF proteins at the cortex and the striatum of 55 day-old WT (n=8), Mecp2 KO (n=6), Mecp2 KO/HTTSD (mimicking the absence of phosphorylation) (n=4) and Mecp2 KO/HTTSA mice (mimicking constitutive phosphorylation) (n=5). Since striatal BDNF depends only on BDNF transport from the cortex, this ratio reflects BDNF transport through corticostriatal pathway (Mann Whitney test). Right: Quantitative analysis of phospho TrkB protein level in striatum of KO WT mice and KO HTTSD mice by immunoblotting. The relative expression levels of phospho TrkB were normalized against GAPDH and are presented as the ratio. (n = 6 mice per group) (Mann Whitney test) Middle: Quantitative analysis of PSD-95 protein level in striatum of Mecp2 KO/HTT WT mice and KO/HTTSD mice by immunoblotting. The relative expression levels of PSD-95 were normalized against GAPDH and are presented as the ratio. (n = 6 mice per group) (Mann Whitney test) Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "(B) Left: We investigated the behavior of Mecp2 KO mice at 35, 45, and 55 days of age and assessed their survival. Right: Mecp2 KO/HTTSD mice (n = 30) and WT mice (n = 17) had a significantly longer lifespan than KO (n=22) or KO/HTTSA mice (n = 21) (Kaplan-Meier survival test). Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "(C) Body weight of 10 WT, 24 KO, 32 KO/HTTSD, and 24 KO/HTTSA mice at P30 and P50 (one-way ANOVA with Tukey's comparison). Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"HTT: 15194"
Figure legend: "(D) Frequency of apnea of 9 WT, 10 KO, 14 KO/HTTSD, and 9 KO/HTTSA mice at P35 and P55. (Kruskal-Wallis test with Dunn's comparison). Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"HTT: 15194"
Figure legend: "(E) Mecp2 KO/HTTSD mice (n=19) performed as well as WT (n=17) at P35 on the accelerating rotarod test and continued to outperform Mecp2 KO (n=16) and Mecp2 KO/ HTTSA (n=13) at P55 (one-way ANOVA with Fisher's LSD test). Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "(F) Time before the onset of spontaneous locomotor activity of 17 WT, 20 KO, 19 KO/HTTSD, and 17 KO/HTTSA mice at P55. (Kruskal-Wallis test with Dunn's comparison). Data information: Data are presented as the means ± SEM. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001 ns = not significant."
NCBI gene IDs:
"HTT: 15194"
Figure legend: "(A) Western blot analysis of DIV 5 cortical neurons transfected with siMecp2 or siControl (siCtl) and treated with 1 µM FK506 or vehicle for 1 h, and quantification of pS421 HTT (n=9 per group). Data are presented as means ± SEM, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, Mann-Whitney test."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "(B) Representative kymographs showing axonal trafficking of BDNF-mCherry-containing vesicles in cortical neurons transfected with siMecp2 or siControl (siCtl) and treated with 1 µM FK506 or vehicle for 1 h. Scale bar = 20 µm."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "(C) Quantification of anterograde, retrograde, mean velocity, linear flow rate and number of BDNF-mCherry-containing vesicles from the data in (A) (siMecp2+Vehicle: n=801 vesicles/95 axons; siMecp2+FK506: n=1020 vesicles/116 axons; siCtl+Vehicle: n=780 vesicles/83 axons; siCtl+FK506: n=1029 vesicles/102 axons). Data are presented as the mean ± SEM of at least three independent experiments (one-way ANOVA with Tukey's comparison). *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001, ns = not significant."
NCBI gene IDs:
"Mecp2: 17257"
Figure legend: "(A) Western blot of HTT S421 phosphorylation. We treated 30-day-old Mecp2 KO mice intraperitoneally with FK506 (5 mg/kg) or vehicle and analyzed brain extracts for endogenous HTT phosphorylation by western blotting, 2 h after administration, using an anti-phospho-HTT-S421 specific antibody. The D7F7 antibody recognizes total HTT. The relative protein level of phospho HTT was normalized on total HTT protein level and are presented as the ratio (KO FK506 n = 4, KO Vehicle n = 4). Data are presented as means ± SEM, *p &lt; 0.05, Mann Whitney test.)"
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "We treated 30-day-old Mecp2 KO mice (n=10) and Mecp2 KO/HTTSA mice (n=10) with 5mg/kg FK506 three times a week by intraperitoneal injection and assessed them in various behavioral tests. (B) FK506-treated KO mice lived longer than vehicle-treated KO mice and FK506-treated KO HTTSA mice (Kaplan-Meier survival test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ns = not significant. Data are means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "We treated 30-day-old Mecp2 KO mice (n=10) and Mecp2 KO/HTTSA mice (n=10) with 5mg/kg FK506 three times a week by intraperitoneal injection and assessed them in various behavioral tests. (C) Body weight of FK506-treated KO, FK506-treated KO HTTSA and vehicle-treated KO mice at P35 and P55 (Mann-Whitney test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ns = not significant. Data are means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "We treated 30-day-old Mecp2 KO mice (n=10) and Mecp2 KO/HTTSA mice (n=10) with 5mg/kg FK506 three times a week by intraperitoneal injection and assessed them in various behavioral tests. (D) Frequency of apnea of FK506-treated KO, FK506-treated KO HTTSA and vehicle-treated KO mice at P35 and P55 (Mann-Whitney test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ns = not significant. Data are means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "We treated 30-day-old Mecp2 KO mice (n=10) and Mecp2 KO/HTTSA mice (n=10) with 5mg/kg FK506 three times a week by intraperitoneal injection and assessed them in various behavioral tests. (E) Motor coordination of FK506-treated KO, FK506-treated KO HTTSA and vehicle-treated KO mice on the accelerating rotarod test at P30 and P50 (Mann-Whitney test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ns = not significant. Data are means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "We treated 30-day-old Mecp2 KO mice (n=10) and Mecp2 KO/HTTSA mice (n=10) with 5mg/kg FK506 three times a week by intraperitoneal injection and assessed them in various behavioral tests. (F) Forelimb strength of FK506-treated KO, FK506-treated KO HTTSA and vehicle-treated KO mice assessed by the grip strength test at P40 and P60 (Mann-Whitney test). Data information: *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ns = not significant. Data are means ± SEM."
NCBI gene IDs:
"HTT: 15194
Mecp2: 17257"
Figure legend: "(A) Phenotype appearance of the Gmlmm1-1 mutant. Typical leaves (left panel; scale bar, 1 cm) and whole plants (right panel; scale bar, 10 cm.) were photographed 60 and 118 days after seeding, respectively. Data information: The experiments were performed three times as biological replicates, with similar results."
NCBI gene IDs:
"Gmlmm1: 100499640"