EMBO/SourceData huggingface dataset:
Figure legend: "(G-H) CFE (colony formation efficiency) of WT and Mettl14 null cells was determined in vitro. Results were quantified and shown as bar graph (F). n=19, P&lt;0.01 (Student's t-test). Error bar represents S.D."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "(I) Fluorescence microscopy demonstrates different  survival capability of WT and Mettl14  inducible KO cells with or without Tamoxifen (TAM) treatment. (J) Ratio of WT and Mettl14  inducible KO cells in the co-culture model was quantified and shown as dot plots. n=8, P&lt;0.01 (Student's t-test) for KO cells with  TAM  treatment compared with WT cells or KO cells without TAM stimulation at both Day 7 and 14. Error bar represents S.D. "
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "C) Deletion of endogenous Pvt1 by an inducible Cas9 (iCas9) system can significantly reduce Pvt1 RNA  level  (quantification from RT-PCR, left panel) and MYC protein level (quantification from  immunoblot, right panel)  expression. n=3, P&lt;0.01 (Student's t-test). Error bar represents S.D."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "D) CFE of WT, Pvt1 inducible KO, and  Pvt1 inducible KO cells rescued with WT or mutant Pvt1 was quantified and presented as bar graph. n=3, **: P&lt;0.01 (Student's t-test). Error bar represents S.D."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "E) Skin organoids derived from WT or Pvt1 inducible KO cells were grafted to nude mice. The regenerated skin was analyzed by  H/E staining."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "F) Epidermal  thickness of WT and  Pvt1  KO skin grafts was quantified and presented as box and whisker plots. The plot indicates the mean (solid diamond within the box), 25th percentile (bottom line of the box), median (middle line of the box), 75th percentile (top line of the box), 5th and 95th percentile (whiskers), 1st and 99th percentile (solid triangles) and minimum and maximum measurements (solid squares). n=6 (biological repeats), P&lt;0.05 (Student's t-test). n=18, P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "H)  Fluorescence microscopy  demonstrates different survival capability of WT and Pvt1 inducible KO cells with or without Doxycycline  (Dox) treatment."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "I) Ratio of WT and Pvt1  inducible KO cells in the co-culture model was quantified and shown as dot plots. n=8, P&lt;0.01 (Student's t-test) for KO cells with Dox treatment compared with WT cells or KO cells without Dox stimulation at both Day 7 and 14. Error bar represents S.D."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "A) Interaction between  Pvt1 and MYC was determined by immunoprecipitation followed with RT-PCR. Note significant decrease in Pvt1  and MYC interaction upon  FTO treatment (left panel), calcium shift-induced differentiation (middle panel, Hi: high calcium), and Pvt1 methylation site mutations (right panel). n=3, P&lt;0.01 (Student's t-test). Error bar represents S.D."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "B) Immunoblots show decreased MYC protein level in differentiated  keratinocytes and Mettl14  KO cells."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "Undifferentiated WT and Mettl14 KO cells (C) were treated with cycloheximide. Cell lysates were collected at 0, 30, 60, 90, 120, 180, and 300 minutes post treatment and subjected to  immunoblotting with different antibodies as indicated."
NCBI gene IDs:
"Mettl14: 210529"
Figure legend: "undifferentiated WT and  Pvt1 KO cells (D) were treated with cycloheximide. Cell lysates were collected at 0, 30, 60, 90, 120, 180, and 300 minutes post treatment and subjected to  immunoblotting with different antibodies as indicated."
NCBI gene IDs:
"Pvt1: 19296"
Figure legend: "(F)  Skin organoids derived from Mettl14 KO cells or KO cells rescued with exogenous expression of MYC were grafted to nude  mice. The regenerated  skin was analyzed by  H/E staining."
NCBI gene IDs:
"Mettl14: 210529
MYC: 4609"
Figure legend: "(G) Epidermal  thickness of KO and KO  skin rescued with MYC expression was quantified and presented as box and whisker plots. The plot indicates the mean (solid diamond within the box), 25th percentile (bottom line of the box), median (middle line of the box), 75th percentile (top line of the box), 5th and 95th percentile (whiskers), 1st and 99th percentile (solid triangles) and minimum and maximum measurements (solid squares). n=6 (biological repeats), P&lt;0.05 (Student's t-test). n=18, P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(H) Number of p63-positive cells in KO and KO skin rescued with MYC expression was quantified and shown as bar graph. n=5, P&lt;0.01 (Student's t-test). Error bar represents S.D."
NCBI gene IDs:
"MYC: 4609"
Figure legend: "(I) Fluorescence microscopy demonstrates different survival capability of WT and Mettl14 KO cells with inducible expression of MYC."
NCBI gene IDs:
"Mettl14: 210529
MYC: 4609"
Figure legend: "(J) Ratio of WT and Mettl14 KO cells with inducible expression of  MYC in the co-culture model was quantified and shown as dot plots. n=8, P&lt;0.01 (Student's t-test) for KO cells without Dox treatment (no  MYC expression) compared with WT cells or KO cells with Dox stimulation (exogenous MYC expression) at both Day 7 and 14. Error bar represents S.D."
NCBI gene IDs:
"Mettl14: 210529
MYC: 4609"
Figure legend: "(D) Representative photographs of fly eyes and quantitation (below) of rough eye  scores with  fly SRSF1 overexpression (dSF2 OE); n=20-32/genotype. Error bars represent mean +/- SD. Data information: For   eye  scoring, target siRNA lines were compared to non-targeting control siRNA lines using a two-tailed student's t test with Welch's correction for multiple comparisons. ∗∗p &lt; 0.01; ∗∗∗p &lt; 0.001; ∗∗∗∗p &lt; 0.0001. Human orthologs of fly  genes are used for labeling."
NCBI gene IDs:
"SRSF1: 43912
SF2: 53443"
Figure legend: "(F) Quantitation of (GGGGCC)28-EGFP rough eye phenotype  with SRSF modifiers (n &gt; 30  flies/ genotype). Error bars represent mean +/- SD. Data information: For  eye scoring, target siRNA lines were compared to non-targeting control siRNA lines using a two-tailed student's t test with Welch's correction for multiple comparisons. ∗∗p &lt; 0.01; ∗∗∗p &lt; 0.001; ∗∗∗∗p &lt; 0.0001. Human orthologs of fly  genes are used for labeling."
NCBI gene IDs:
"EGFP: 
SRSF: 53443"
Figure legend: "(G) Representative photographs of  fly eyes expressing GMR-GAL4 driven (GGGGCC)28-EGFP  at 29˚C along with the quantifications of necrosis and eye width. n=28-30/genotype. Error bars represent mean +/- SD."
NCBI gene IDs:
"EGFP: 
GAL4: "
Figure legend: "(H-I) Survival  assays of flies expressing (CGG)90-EGFP under Tub5-GS (H) and ELAV-GS (I) drivers with control or SRSF1  siRNAs. Expression of (CGG)90-EGFP was initiated with addition of drug starting 1 day post eclosion and continued through experiment (Log-rank Mantel-Cox test; n=98-101/genotype for Tub-GS and n=120-141/genotype for  ELAV-GS flies) ∗p &lt; 0.05, ∗∗p &lt; 0.01. (J) Survival assays of (GGGGCC)28-EGFP expressing  fly  under Tub5-GS driver (Log-rank Mantel-Cox test; n=71-93/genotype) with control or SRSF1 siRNAs. ∗∗p &lt; 0.01. "
NCBI gene IDs:
"EGFP: 
ELAV: 31000
SRSF1: 43912
Tub: 40554
Tub5: 40554"
Figure legend: "(A, B) Representative (A) photographs of  fly eyes  and (B) quantitation with siRNA mediated knockdown of SRPK1 (dSRPK1); n=30-34/genotype. Error bars represent mean +/- SD. Data information: Statistical analysis was performed using two-tailed Student's t test with Welch's correction, ∗∗∗p &lt; 0.001; ∗∗∗∗p &lt; 0.0001."
NCBI gene IDs:
"SRPK1: 36706"
Figure legend: "(C) Representative photographs of fly eyes expressing GMR-GAL4 driven (GGGGCC)28-EGFP  with siRNA mediated knockdown of  SRPK1 or disruption by insertion. (D) Quantitation of rough eye phenotypes. t-test with Welch corrections for comparisons with the control; n= 31-34 flies/ genotype. Error bars represent mean +/- SD. Data information: Statistical analysis was performed using two-tailed Student's t test with Welch's correction, ∗∗∗p &lt; 0.001; ∗∗∗∗p &lt; 0.0001."
NCBI gene IDs:
"EGFP: 
GAL4: 
SRPK1: 36706"
Figure legend: "(E) Representative external eye imaging for detection of GFP aggregates caused by (CGG)90-EGFP transgene expression (top). Converted images used to quantify total intensity of  GFP puncta (bottom). (F) Depletion of SRSF1 or SRPK1 by RNAi results in reduced (CGG)90-EGFP  puncta compared to control siRNA as quantified by total intensity (a.u. = arbitrary unit). n=13-15 flies/genotype. Error bars represent mean +/- SD. Data information: Statistical analysis was performed using two-tailed Student's t test with Welch's correction, ∗∗∗p &lt; 0.001; ∗∗∗∗p &lt; 0.0001. "
NCBI gene IDs:
"EGFP: 
SRSF1: 43912
SRPK1: 36706"
Figure legend: "(B) Anti-FLAG immunoblot of DMSO and SRPIN340 pre-treated HEK293T cells expressing AUG-nLuc-3xFLAG control or CGG-nLuc-3xFLAG RAN translation reporters. β-Actin is used as a loading control. To prevent signal saturation, AUG-nLuc lysate was diluted 1:3 in sample buffer prior to loading (n=3 biological replicates). Schematics of the AUG-nLUC-3xFLAG and +1CGG(100)-nLuc-3xFLAG reporters presented on top. Data information: Error bars represent mean +/- SD. Statistical analysis was performed using two-tailed Student's t test with Welch's correction. ∗p &lt; 0.05; ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001 and ∗∗∗∗p &lt; 0.0001. To prevent over-exposure, the AUG-nLuc lysate was diluted 1:3 in sample buffer."
NCBI gene IDs:
"FLAG: 
Luc: 
LUC: "
Figure legend: "(D) Anti-FLAG immunoblot  of DMSO  and SRPIN340 pre-treated HEK293T cells expressing GGGGCC-nLuc-3xFLAG (GA70) and +2CGG-nLuc-3xFLAG (FMRpolyA) RAN translation reporters (n=3 biological replicates). Schematics of the GA70 (GGGGCCx70) and +2CGG reporters presented on top. Data information: Error bars represent mean +/- SD. Statistical analysis was performed using two-tailed Student's t test with Welch's correction. ∗p &lt; 0.05; ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001 and ∗∗∗∗p &lt; 0.0001."
NCBI gene IDs:
"FLAG: 
Luc: 
FMR: 2332"
Figure legend: "(E) Immunoblot of DMSO and SPHINX31 pre-treated  HEK293T cells expressing AUG-nLuc-3xFLAG control or CGG-nLuc-3xFLAG RAN translation reporters. (n=3 biological replicates). (F) Anti-FLAG of DMSO and SPHINX31 pre-treated HEK293T cells expressing  GGGGCC-nLuc-3xFLAG (GA70) and +2CGG-nLuc-3xFLAG (FMRpolyA) RAN translation reporters (n=3 biological replicates). Data information: Error bars represent mean +/- SD. Statistical analysis was performed using two-tailed Student's t test with Welch's correction. ∗p &lt; 0.05; ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001 and ∗∗∗∗p &lt; 0.0001. "
NCBI gene IDs:
"FLAG: 
Luc: 
FMR: 2332"
Figure legend: "(C, D) Expression of GGGGCC-nLuc-3xFLAG (C) and +2CGG-nLuc-3xFLAG (D) RAN translation reporters in HEK293T  cells treated with 2 μM TG (for stress induction) analyzed by immunoblot  (n=3 biological replicates). To evaluate effects of  SRPK1 inhibition cells were pre-treated with  DMSO or SRPIN340 before reporter transfection. Data information: Error bars represent mean +/- SD. Two-tailed Student's t test with Welch's correction. ∗p &lt; 0.05; ∗∗p &lt; 0.01; ∗∗∗p &lt; 0.001 and ∗∗∗∗p &lt; 0.0001."
NCBI gene IDs:
"FLAG: 
Luc: "
Figure legend: "(D) Anti-FLAG immunoblot blot  of DMSO  and SRPIN340 pre-treated HEK293T cells transfected with in vitro transcribed CGG-nLuc-3xFLAG reporter RNA. β-Actin is used as a loading control. Error bars represent mean +/- SD (n=6 biological replicates). Statistical analysis was performed using Student's t test with Welch's correction. ∗p &lt; 0.05"
NCBI gene IDs:
"FLAG: 
Luc: "
Figure legend: "(A, B) Pharmacological targeting of SPRK1 with (A) 40 μM SRPIN340  or (B) 8 μM SPHINX31 reduced the cumulative risk of death in +1(CGG)100-EGFP (encoding  FMRpolyG) expressing neurons. n= # of  neurons quantified for each condition; Cox proportional hazard analysis, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"EGFP: "
Figure legend: "(A-B) Proteins and RNA samples of colon tissue were extracted from mice with the indicated treatments. (B) mRNA expression levels of IL-6, IL-12p35 and IL-23p19 were assessed by quantitative RT-PCR and normalized to TBP (n=4-6). Results represent two or more independent experiments."
NCBI gene IDs:
"TBP: 
IL-12p35: 16159
IL-23p19: 83430
IL-6: 16193"
Figure legend: "(C) Real-time PCR analysis showing mRNA expression levels of IL-6, IL-12p35 and IL-23p19 of FACS isolated monocytes collected from mice with the indicated treatments. Data are obtained from two to three independent experiments (n=6-7 mice pooled per group)."
NCBI gene IDs:
"IL-12p35: 16159
IL-23p19: 83430
IL-6: 16193"
Figure legend: " C Raptor-depleted, WT cortical neurons were imaged by 2P-FLIM before and 30 minutes after treatment with insulin. Inhibiting mTORC1 by depleting Raptor with shRNA made mitochondria insensitive to insulin. Shown here are average data from 4 fields of view of a single replicate out of a total of 3 replicates. Western blots are representative of 3 independent assays. Statistical analyses were performed using Student's two-tailed unpaired t-test "
NCBI gene IDs:
"Raptor: 74370"
Figure legend: " C Reducing Bcl-xL expression in WT mouse cortical neurons does not affect NiMA. Western blots are representative of 3 independent assays. Statistical analyses were performed using Student's two-tailed unpaired t-test "
NCBI gene IDs:
"Bcl-xL: 12048"
Figure legend: "C WT mouse cortical neurons expressing either Flag-Raptor H-Ras25 or Flag-Raptor Rheb15 were serum-starved in AβO-containing Hank\"s balanced salt solution for 2 hours. Next, the cells were pulse-labeled for 3 hours with EdU. Note that forcing mTORC1 to the plasma membrane (Flag-Raptor H-Ras25) yielded an ~80% increase in EdU incorporation into mtDNA in the presence of AβOs regardless of whether or not the neurons had been stimulated with insulin+R+L. In contrast, EdU uptake into mitochondrial replisomes was not stimulated by AβOs in neurons whose mTORC1 was forced to lysosomes (Flag-Raptor-Rheb15). Error bars represent ±"
NCBI gene IDs:
"H-Ras25: 15461
Rheb15: 19744
Raptor: 74370"
Figure legend: " A Unmodified ReNcell VM human neuronal progenitor cells were co-plated with comparable Ren-GFP or Ren-APPSL cells into 3-dimensional Matrigel matrices, and differentiated into neurons for 60 days. Cytosolic GFP is produced by both Ren-GFP and Ren-APPSL cells, and the latter also produce pathogenic human APP with the Swedish and London mutations, and secrete Aβ that becomes entrapped in the Matrige Following neuronal differentiation, 2P-FLIM was used to monitor NiMA in the ReNcell VM neurons, which were discriminated from Ren-GFP and Ren-APPSL neurons because they lack GFP. Note that NiMA was observed in ReNcell VM neurons when they were co-cultured with Ren-GFP neurons, but not when they were co-cultured with Ren-APPSL neurons. Each colored line refers to a single field of view containing 5-15 cells, and each pair of solid and dotted lines of the same color refers to the same field of view. Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"APP: 
APPSL: 351"
Figure legend: " B ReNcell VM cells were differentiated into neurons for 30 days in 2D cultures. Subsequent AβO exposure for 16 hours blocked NiMA, but expression of Flag-Raptor-Rheb15, which forces mTORC1 to lysosomes, prevented NiMA inhibition by AβOs. Each colored line refers to a single field of view containing 5-15 cells, and each identically colored pair of solid and dotted lines refers to the same field of view. Flag-Raptor -Rheb15 expression was confirmed by western blot­ting. Statistical analyses were performed using Student's two-tailed unpaired t-test. Western blots are representative of 3 independent assays"
NCBI gene IDs:
"Rheb15: 6009
Raptor: 57521"
Figure legend: " A Cortical mouse neurons from tau knockout (KO) mice were serum-starved in Hank's balanced salt solution for 2 hours. The cells were then imaged for NiMA, after which insulin or amino acids were immediately added, and the cells were then imaged again 30 minutes later Data information: Each colored lin refers to a single field of view containing 2-15 cells, and each pair of solid and dotted lines (same color) refers to the same field of view. Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"tau: 17762"
Figure legend: " B Cortical mouse neurons from tau KO mice were treated with AβOs for 18 hours before being serum-starved in AβO-containing Hank's balanced salt solution for 2 hours. The cells were then imaged for NiMA, after which insulin was immediately added, and the cells were then imaged again 30 minutes later. Note that NiMA occurred in the absence or presence of AβOs, which inhibited NiMA in wild type human neuron Data information: Each colored lin refers to a single field of view containing 2-15 cells, and each pair of solid and dotted lines (same color) refers to the same field of view. Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"tau: 17762"
Figure legend: "Wild type mouse cortical neurons depleted of Nprl with shRNA had mitochondria that were insensitive to amino acids (R+L Each colored line refers to a single field of view containing 5-15 cells, and each pair of solid and dotted lines (same color) refers to the same field of view. Knockdown efficiency was monitored by western blotting of phosphorylated ribosomal S6 protein, a downstream target of the mTORC1-S6K pathway. Western blots are representative of 3 independent assays. Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"Nprl: 17168"
Figure legend: "Wild type mouse cortical neurons depleted o Tsc2 with shRNA had mitochondria tha were insensitive t insuli Each colored line refers to a single field of view containing 5-15 cells, and each pair of solid and dotted lines (same color) refers to the same field of view. Knockdown efficiency was monitored by western blotting of phosphorylated ribosomal S6 protein, a downstream target of the mTORC1-S6K pathway. Western blots are representative of 3 independent assays. Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"Tsc2: 22084"
Figure legend: "(D Human fibroblasts deficient in Tsc2 (TSC2 Δexons1-14) were imaged before and 30 minutes after insulin addition. Lack of Tsc2 made mitochondria insensitive to insulin Cultures were serum-starved in Hank's balanced salt solution for 2 hours before stimulation with insulin or amino acids, and were imaged before and 30 minutes after stimulation. Each colored line refers to a single field of view containing 2-5 cells, and each pair of solid and dotted lines of the same color refers to the same field of view Statistical analyses were performed using Student's two-tailed unpaired t-test"
NCBI gene IDs:
"Tsc2: 7249
TSC2: 7249"
Figure legend: "(E) Re-expression of human Tsc2 rescues mitochondrial responses to insulin. Cultures were serum-starved in Hank's balanced salt solution for 2 hours before stimulation with insulin or amino acids, and were imaged before and 30 minutes after stimulation. Each colored line refers to a single field of view containing 2-5 cells, and each pair of solid and dotted lines of the same color refers to the same field of view. Re-expression of the Flag-WT Tsc2 was corroborated by indirect immun­o­fluorescence (IF). Statistical analyses were performed using Student's two-tailed unpaired t-test "
NCBI gene IDs:
"Tsc2: 7249"
Figure legend: "(A) Path-seq profiles of desA1, desA2 in MTB infected BMDMs. Error bars show the standard deviation from three biological samples. Representative results from two experiments are presented."
NCBI gene IDs:
"desA1: 885444
desA2: 885339"
Figure legend: "(B) Upper panel: dissolved oxygen curve over 120 h time course showing controlled depletion (1), sustained hypoxia (2) and controlled reaeration (3). Points represent the average of three biological replicates and were measured via fiber optic technology that non-invasively probes oxygen levels in the culture (PreSens Precision Sensing GmbH). Lower panel: expression profiles (RNA-seq) of desA1 and desA2 over the time course and oxygen levels. Error bars show the standard deviation from three biological samples."
NCBI gene IDs:
"desA1: 885444
desA2: 885339"
Figure legend: "(C) Rv0681 regulon genes differentially expressed in vitro and the evidence for predicted high Rv0681 activity (induced expression of target genes). (D) Zur regulon genes differentially expressed in vitro and the evidence for decreased Zur activity (de-represses target genes). (E) Rv0691c regulon genes differentially expressed in vitro and in vivo; evidence for increased Rv0691c activity (increased up- and down-regulation of target genes) at 24 h from both in vitro and in vivo infection."
NCBI gene IDs:
"Rv0681: 888239
Rv0691c: 888296
Zur: 886009"
Figure legend: "(B) Plot of read pile-ups from MSM with inducible overexpression of MSMEG_0916 shows ChIP-binding in the promoters of desA1 and desA2."
NCBI gene IDs:
"MSMEG_0916: 4531289
desA2: 4532334
desA1: 4533322"
Figure legend: "Boxplots representing RPKM values from RNA-seq of MSM with inducible overexpression of MSMEG_0916. Significant log2 fold change (FC) between uninduced (-ATc) and induced (+ATc) samples for MSMEG_0916 (log2 FC = 4.99), desA1 (log2 FC = -1.32) and desA2 (log2 FC = -1.8) with multiple hypothesis adjusted P-values &lt; 0.0001. Data is from three biological samples (induced) and nine biological samples (uninduced)."
NCBI gene IDs:
"MSMEG_0916: 4531289
desA2: 4532334
desA1: 4533322"
Figure legend: "(A) Serial ten-fold dilutions of MTB H37Rv wild type (wt) and MTB with inducible overexpression of Rv0472c were spotted on 7H10 agar plates with or without ATc."
NCBI gene IDs:
"Rv0472c: 886308"
Figure legend: "(B) Argentation TLC of 14C-labeled methyl esters of mycolic acids (MAMEs) obtained from apolar lipids and delipidated cell wall fractions of MSM wt and MSM with inducible overexpression of MSMEG_0916. The α, αʹ, epoxy (e) and cyclopropanated α- (X1) MAMES species are labeled. Faster-migrating species that co-migrated with α-MAMES and accumulate with induced MSMEG_0916 overexpression are indicated as X2 and X3."
NCBI gene IDs:
"MSMEG_0916: 4531289"
Figure legend: "(C) BCG wt and BCG with inducible overexpression of Rv0472c cultures, labeled with 14C-acetate, were induced (+ATc) or uninduced (-ATc) for 4 h or 8 h. The total FAMEs and MAMEs were extracted and analyzed by autoradiography-TLC using equal counts (15,000 cpm) for each lane."
NCBI gene IDs:
"Rv0472c: 886308"
Figure legend: "(A) Quantitative analysis of the Aβ1-40 protein in the TBS (*p=0.0212), Triton-X (p=0.0544) and SDS (**p=0.0063) fractions of brain homogenates from male (n=7) and female (n=10) APP23 mice. Mean ± s.e.m., statistical analysis: two-tailed unpaired t-test."
NCBI gene IDs:
"APP: 351"
Figure legend: "(B) Stereological analysis of cortical area covered by 4G8-positive plaques in male (n=10) and female (n=8) APP23 mice (left) and representative images (right), scale bar = 500 µm. Mean ± s.e.m., statistical analysis: two-tailed unpaired t-test, ***p=0.0004."
NCBI gene IDs:
"APP: 351"
Figure legend: "(C) Fluorescence intensity based analysis of pFTAA-stained Aβ plaques in the cortex of male (n=10) and female (n=8) APP23 mice (left) and representative images (right), scale bar = 1 mm. Mean ± s.e.m., statistical analysis: two-tailed unpaired t-test, **p=0.0011."
NCBI gene IDs:
"APP: 351"
Figure legend: "(D) Stereological analysis of cortical area covered by Congo Red-positive plaques in male (n=10) and female (n=8) APP23 mice (left) and representative images (right), scale bar = 500 µm. Mean ± s.e.m., statistical analysis: two-tailed unpaired t-test, ***p=0.0001."
NCBI gene IDs:
"APP: 351"
Figure legend: "(E) Native filter assay analysis of TBS (p=0.2453), Triton-X (p=0.0604), SDS (*p=0.0196) and formic acid (FA) (**p=0.0057) fractions from male (n=8) and female (n=7) APP23 mouse brain homogenates. Mean ± s.e.m., statistical analysis: two-tailed unpaired t-test between the same fractions. Data points were taken from graphs analysing APP23p40+/+ against APP23p40-/- mice."
NCBI gene IDs:
"APP: 351
p40: 16160"