EMBO/SourceData huggingface dataset:
Figure legend: "D HeLa cells expressing Dox-inducible RIPK3 WT, D160N, K42R, K351, K518R, 2xKR or 3xKR were pre‑incubated with 1 µg/ml Dox, 20 µM Dab, or 10 µM NSA overnight, followed by pre-treatment with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 4 h. Cell death was measured by quantification of PI-positive nuclei."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "E HeLa cells expressing Dox-inducible RIPK3 mutants were treated with 1 µg/ml Dox overnight. Protein levels of inducible RIPK3 expression were analyzed by Western blotting. GAPDH served as loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "A HeLa cells expressing Dox-inducible RIPK3 WT, K518R or 3xKR were treated with 1 µg/ml Dox and/or 10 µM NSA and 20 µM Dab overnight. Protein levels of inducible RIPK3 expression and phosphorylated MLKL were examined by Western blotting. β-Actin was used as a loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "B HeLa cells expressing Dox-inducible RIPK3 WT or 3xKR were treated with 1 µg/ml Dox overnight before pre-treatment with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 1, 2, 3 and 4 h. Protein levels of inducible RIPK3 expression and phosphorylated MLKL were analyzed by Western blotting. β-Actin served as loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "C HeLa cells expressing Dox-inducible RIPK3 WT, 2xKR or 3xKR were treated with 1 µg/ml Dox overnight before pre-treatment with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 2 h. Protein levels of inducible RIPK3 expression were analyzed by Western blotting. β-Actin served as loading control. D HeLa cells expressing Dox-inducible RIPK3 WT, K518R or 3xKR were treated with 1 µg/ml Dox overnight before pre-treatment with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 2 h. Whole cell lysates were generated using RIPA lysis buffer containing 2% SDS. Protein levels of inducible RIPK3 expression were analyzed by Western blotting. β-Actin served as loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "E HeLa cells expressing Dox-inducible RIPK3 WT, D160N, K518R or 3xKR were incubated overnight with 1 µg/ml Dox and pre-treated with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 1 and 2 h. Strep-RIPK3 was immunoprecipitated by using anti-Strep-beads. Co-immunoprecipitated phosphorylated MLKL and RIPK1, as well as protein expression of indicated proteins were analyzed by Western blotting. β-Actin served as a loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "F HeLa cells expressing Dox-inducible RIPK3 WT, K518R or 3xKR were incubated with 1 µg/ml Dox and transfected with HA-ubiquitin for 24 h, as indicated. Cells were pre-treated with 20 µM zVAD.fmk, 5 µM BV6 for 1 h. After pre-treatment, 10 ng/ml TNFα were added for 2 h. HA-ubiquitin was immunoprecipitated using anti-HA-beads and detection of indicated proteins was performed by Western blotting. β-Actin served as loading control for the input, whereas HA-ubiquitin levels served as loading control for immunoprecipitated ubiquitin."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "A HT‑29 RIPK3 KO cells re-expressing Dox-inducible WT RIPK3 or the indicated RIPK3 mutants were incubated with 1 µg/ml Dox overnight. Cells were treated with 20 µM zVAD.fmk, 5 µM BV6 and 10 ng/ml TNFα for 4 h. Cell death was measured by analysis of PI-positive nuclei."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "B HT‑29 RIPK3 KO cells re-expressing Dox-inducible WT RIPK3 or the indicated RIPK3 mutants were incubated with 1 µg/ml Dox overnight, as indicated. Cells were treated with 20 µM zVAD.fmk, 5 µM BV6 and 10 ng/ml TNFα for 2 h and analyzed by Western blotting for RIPK3 and phosphorylated MLKL expression levels. β-Actin served as a loading control."
NCBI gene IDs:
"RIPK3: 11035"
Figure legend: "(C) hematoxylin-eosin staining  with scale bar of 50 µm of the back skin   of ILEIind and K5-ILEIind mice  kept on normal or doxycycline  diet and treated with acetone  or TPA  for 5 days (n=2-5; 3 independent experiments)."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(D) mean epidermal  thickness ±SEM of the  back skin  of ILEIind and K5-ILEIind mice  kept on normal or doxycycline  diet and treated with acetone  or TPA  for 5 days (n=2-5; 3 independent experiments). Statistical significance was determined by one-way ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; ***p,0.001)."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(E) Representative images of ILEI immunohistochemistry  on sections of back skin  of ILEIind and K5-ILEIind mice  kept on normal or doxycycline  diet upon 5 days of treatment with acetone  or TPA. Scale bar 50 µm. Inlets show a magnification of the marked regions, scale bar 20 µm."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(A) Representative images of Ki67 immunohistochemistry  on back skin  sections of K5-ILEIind and ILEIind mice  kept on normal or doxycycline  diet and treated with acetone  or TPA  for 5 days. Scale bar: 100 µm. (B) Percentage of Ki67  positive cells in the epidermis  shown as mean ±SEM and quantified on samples described in panel A (n=1-5; 3 independent experiments). Data information: Statistical significance was determined by one-way ANOVA (B at ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p,0.001)."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(D) Keratin 16 (K16)  western blot  analysis (left) and quantification (right) of proteins extracted from back skin  of ILEIind and K5-ILEIind mice  kept on doxycycline  and treated with acetone  or TPA  for 5 days (n=3). Vinculin  was used as loading control. Data information: Statistical significance was determined by one-way ANOVA at ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p,0.001)."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(G) Mean fold change in mRNA expression  ±SEM of K5  and K10  in primary keratinocytes  described in panel F (n=5-6; 2 independent experiments). Data information: Statistical significance was determined by two-way ANOVA (G) at ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p,0.001)."
NCBI gene IDs:
"K10: 16661
K5: 110308"
Figure legend: "Mean fold change ±SEM in mRNA expression  of Tnfa, Il1α  and Cxcl1  (E) in freshly sorted keratinocytes  enriched for the inter-follicular epithelium (IFE) and hair follicles  (HF) from acetone  and TPA  treated back skin in mice  supplemented with doxycycline  and treated with acetone  or TPA  for 96 hours (n=3 (E), independent experiments). Data information: statistical significance was determined by one-way ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001). If significance levels were different for the pairwise comparisons with combined marking, asterisks, valid only for a subset of the pairs were put into brackets."
NCBI gene IDs:
"Cxcl1: 14825
Il1α: 16175
Tnfa: 21926"
Figure legend: "(G-I) Mean fold change ±SEM in mRNA expression  of (G) Tnfa, (H) Il1α  and (I) Cxcl1  in  primary keratinocytes treated with acetone  and TPA  and with increasing concentrations (100ng/ml, 250ng/ml, 500ng/ml, 1000ng/ml) of murine  recombinant ILEI  (mILEI) or dimerization-disabled ILEI   (mILEICA) or with empty-vector (empty) for 72 hours (n=3, standing for independent keratinocyte  cultures from three mice). Data information: statistical significance was determined by one-way ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001). If significance levels were different for the pairwise comparisons with combined marking, asterisks, valid only for a subset of the pairs were put into brackets."
NCBI gene IDs:
"Cxcl1: 14825
Il1α: 16175
Tnfa: 21926"
Figure legend: "(A-C) Representative images of (A) Phospho-STAT3  (Tyr727), (B) Phospho-Erk1/2 and (C) Phospho-Akt immunofluorescence  on thin sections of acetone  and TPA  treated back skin of  ILEIind and K5-ILEIind mice  kept on doxycycline  diet. Scale bar, 50 µm."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(F) Western blot  analysis of STAT3  (Ser727 and Tyr705), Akt  and Erk1/2 phosphorylation  levels in primary Dox-induced K5-ILEIind keratinocytes  treated with acetone  or TPA  for 4 hours in the presence of the inhibitors STATTIC  (10µM), LY92004  (10µM) and UO126  (10µM). DMSO  was used as vehicle control. Vinculin  was used as loading control. Lanes are from non-continuous parts of the same gel."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "(B) hematoxylin-eosin staining  with a scale bar of 50 µm and (C) mean epidermal  thickness ±SEM of the back skin  of ILEIfl/fl and ILEI∆Ep mice  treated with acetone  or TPA  for 5 days (n=3-8; 3 independent experiments). Data information: In C statistical significance was determined one-way ANOVA with Tukey multiple comparison test (C marked with asterisks (*p&lt;0.05; **p&lt;0.01; ****p&lt;0.0001). If significance levels were different for the pairwise comparisons with combined marking, asterisks, valid only for a subset of the pairs were put into brackets."
NCBI gene IDs:
"ILEI: 27999"
Figure legend: "(F-H) (F) Representative images of MPO+ microabscesses   and neutrophils, (G) mean number ±SEM of MPO-positive microabsesses/cm skin  section and (H) mean number of neutrophils  (MPO+ cells)/mm2 epidermal-dermal  area ±SEM on thin sections of acetone  and TPA  treated back skin of ILEIfl/fl and  ILEI∆Ep mice  (n=6-8 (G) and n=2-8 (H); 3 independent experiments). Scale bar, 100 µm. Data information: In G and H, statistical significance was determined one-way ANOVA with Tukey multiple comparison test H) or by Student's t-test (G) and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ****p&lt;0.0001). If significance levels were different for the pairwise comparisons with combined marking, asterisks, valid only for a subset of the pairs were put into brackets."
NCBI gene IDs:
"ILEI: 27999"
Figure legend: "Mean fold change ±SEM in mRNA expression  of uPA  and uPAR  (D) in freshly sorted keratinocytes  enriched for the interfollicular epithelium from acetone  and TPA  treated back skin, (E) in primary keratinocytes isolated from ILEIind and K5-ILEIind mice, supplemented with doxycycline  and treated with acetone  or TPA  for 96 hours (n=3 (D), n=6 (E 1-2 independent experiments). Data information: In D,E Statistical significance was determined by one-way ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001)."
NCBI gene IDs:
"ILEI: 27999
K5: 281268
uPA: 18792
uPAR: 18793"
Figure legend: "Mean fold change ±SEM in mRNA expression  of uPA  and uPAR  (F) in primary wild type keratinocytes  treated with acetone  and TPA  and with murine  recombinant wild type ILEI  (mILEI) for 8 and 96 hours n=6 F); 1-2 independent experiments). Data information: Statistical significance was determined by one-way ANOVA with Tukey multiple comparison test and marked with asterisks (*p&lt;0.05; **p&lt;0.01; ***p&lt;0.001; ****p&lt;0.0001)."
NCBI gene IDs:
"uPA: 18792
uPAR: 18793"
Figure legend: "(L) ILEI Western blot  analysis of conditioned media harvested from  primary keratinocytes of ILEIind and K5-ILEIind mice  after 48-hours doxycycline  induction and treatment with TPA  and DMSO  or indicated concentrations of UK371804. Loading was normalized to cell count, numbers indicate relative intensities. Lanes are from non-continuous parts of the same gel."
NCBI gene IDs:
"ILEI: 27999
K5: 281268"
Figure legend: "B-G' Confocal images of the AIY labeled with synaptic vesicle marker GFP::RAB-3 (B-G, green) and active zone marker GFP::SYD-1 (B'-G', pseudo-red) in wild-type (B, B'), cwn-2(ok895) (C, C'), cfz-2(ok1201) (D, D'), pop-1(hu9) (E, E'), and sys-1 RNAi (G, G') animals. H-H' Quantification of the fluorescence intensity of AIY RAB-3 (H) and SYD-1 (H') for the indicated genotypes."
NCBI gene IDs:
"cfz-2: 178768
cwn-2: 177870
pop-1: 171849
sys-1: 172859"
Figure legend: "I-M' Confocal images of AIY labeled with mCherry::RAB-3 (pseudo-GFP) (I-M), or GFP::RAB-3 (I'-M') of tissue-specific RNAi treatment for control (I-I'), cwn-2(J-J'), cfz-2(K-K'), sys-1(L-L') and pop-1(M-M'). N-N' Quantification of the fluorescence intensity of AIY mCherry::RAB-3 or GFP::RAB-3 for the indicated RNAi treatment."
NCBI gene IDs:
"cfz-2: 178768
cwn-2: 177870
pop-1: 171849
sys-1: 172859"
Figure legend: "A Quantification of the fluorescence intensity of AIY GFP::RAB-3 in wild-type , egl-3(n589) and aex-5(sa23) mutants."
NCBI gene IDs:
"aex-5: 173358
egl-3: 179412"
Figure legend: "C Quantification of the fluorescence intensity of AIY GFP::RAB-3 for the indicated RNAi treatment. Candidates are intestinal expressed nlp or flp."
NCBI gene IDs:
"flp: 182944"
Figure legend: "Confocal images of AIY labeled with GFP::RAB-3 in wild-type (E) with a wild type nlp-40 transgene."
NCBI gene IDs:
"nlp-40: 171591"
Figure legend: "Confocal images of AIY labeled with nlp-40(tm4085) (F), nlp-40(vj3) (G), nlp-40(tm4085) (H) or nlp-40(vj3) (I) with a wild type nlp-40 transgene. J Quantification of the fluorescence intensity of AIY GFP::RAB-3 for the indicated genotypes. Transgenic data are averaged from at least two independent lines."
NCBI gene IDs:
"nlp-40: 171591"
Figure legend: "A-C Quantification of the fluorescence intensity of AIY presynaptic marker GFP::RAB-3 (A, B) and nlp-40 mRNA level (C) for the indicated genotypes. The AIY synaptic region was quantified for the synaptic intensity analysis."
NCBI gene IDs:
"nlp-40: 171591"
Figure legend: "D-I Confocal images of the transcriptional nlp-40 reporter (Pnlp-40::GFP) in wild-type (D), cwn-2(ok895) (E), cfz-2(ok1201) (F), pop-1(hu9) (G), and control or sys-1 RNAi (H and I) animals at adult Day 1 stage. J Quantification of the Pnlp-40::GFP fluorescence intensity for the indicated genotypes."
NCBI gene IDs:
"GFP: 
cfz-2: 178768
cwn-2: 177870
nlp-40: 171591
pop-1: 171849
sys-1: 172859"
Figure legend: "K-P Confocal images of an intestinal-specific reporter ERM-1::GFP in wild type (K), cwn-2(ok895) (L), cfz-2(ok1201) (M), pop-1(hu9) (N), and control or sys-1 RNAi (O and P) animals at the adult Day1 stage. Q Quantification of the ERM-1::GFP fluorescence intensity for the indicated genotype."
NCBI gene IDs:
"cfz-2: 178768
cwn-2: 177870
pop-1: 171849
sys-1: 172859"
Figure legend: "A Diagram of aex-2 genomic structure. The boxes and lines represent exons and introns. Black and gray indicate coding sequence and UTRs. The white asterisk marks the location of the R232Q mutation site of sa3 allele. B-K Confocal images of AIY presynaptic site labeled with GFP::RAB-3 in wild type(B), nlp-40(tm4085)(C), aex-2(sa3)(D), nlp-40(tm4085); aex-2(sa3) (E) over-expression NLP-40 in aex-2(sa3) mutants(F) and aex-2(sa3) with a wild-type aex-2 transgene driven by the endogenous promoter (Paex-2) (G), neuron-specific promoter (Prab-3) (H) , AIY-specific promoter (Pttx-3) (I) intestinal-specific promoter (Pges-1) (J) or muscle-specific promoter (Pmyo-3)(K). L-M Quantification of the fluorescence intensity of AIY GFP::RAB-3 for the indicated genotypes. Each dot represents one animal. Data were collected from at least three independent experiments. Transgenic data are averaged from at least two independent lines. tg: Paex-2::aex-2, Pttx-3::aex-2 or Pges-1::aex-2 transgene, +: wild type ; -: mutant or without tg. The AIY synaptic region was quantified for the synaptic intensity analysis."
NCBI gene IDs:
"aex-2: 188491
ges-1: 178633
myo-3: 179676
nlp-40: 171591
NLP-40: 171591
rab-3: 173971
ttx-3: 181357"
Figure legend: "N-Q' Confocal images of Paex-2::mNeonGreen of whole animal with bright field (N), the head region labeled with Paex-2::mNeonGreen and Pttx-3::mCherry with green channel (O), red channel (P) and the merged channels (Q). (O'-Q') are the cross sections corresponding to the dashed line sites."
NCBI gene IDs:
"mCherry: 
mNeonGreen: 
aex-2: 188491
ttx-3: 181357"
Figure legend: "A-A' Representative confocal images showing inactive (A) and active(A') state of AIY::GCaMP6s (with mod-1 promoter) colabeled with mCherry (with ttx-3 promoter) (red). Dashed ovals mark the AIY zone 2 region where GCaMP was quantified."
NCBI gene IDs:
"mCherry: 
mod-1: 179269
ttx-3: 181357"
Figure legend: "B-G Confocal images of Prab-3::GFP::RAB-3 in the nerve region corresponding to the dashed box in (A) of wild type (B), cwn-2(ok895) (C), cfz-2(ok1201) (D), pop-1(hu9) (E), nlp-40(tm4085) (F) and aex-2(sa3) (G) mutants. H-I Quantification of the GFP::RAB-3 intensity in the nerve ring synaptic region for the indicated genotypes. Each dot represents one animal. Data were collected from at least three independent experiments. Transgenic data are averaged from at least two independent lines."
NCBI gene IDs:
"aex-2: 188491
cfz-2: 178768
cwn-2: 177870
nlp-40: 171591
pop-1: 171849"
Figure legend: "G, Representative confocal images of NPC1, internalized integrin β1 and pFAK localization. Stably NPC1-GFP expressing cells were loaded with 2 μg/mL integrin β1 antibody for 30 min before fixation and  stained with pFAK antibody followed by secondary antibodies. Dashed line indicates the cell edge. H, Quantification of G using Mander's colocalization for the indicated markers. Mean ± SD, n = 5 cells. Cells were treated with 5 % LPDS for 1 day and then without (-) or with (+) 50 μg/ml  LDL for 1h. "
NCBI gene IDs:
"GFP: 
NPC1: 4864"
Figure legend: "F, Schematic outline for plasma membrane D4H labeling in combination with ORP2 degron system, and representative widefield epifluorescent images of co-plated cells after 4 h of LDL loading. Asterisk indicates ORP2-depleted degron cells. Dashed lines separate ORP2  expressing and depleted cells. G, Quantification of F. Mean ± SD, n = 20 fields of cells pooled from 2 independent experiments. Student's t-test. "
NCBI gene IDs:
"ORP2: 9885"
Figure legend: "A, Control and degron ORP2 cells were co-plated, and after 1 day incubation in 5 %  LPDS, cells were loaded with  LDL (+LDL) and IAA (+IAA; ORP2 depletion) for the indicated  times. Intracellular cholesterol was labeled with D4H  and imaged by widefield epifluorescence microscopy. Asterisk indicates region of ORP2-depleted cells. CM, complete medium. Dashed lines separate ORP2 expressing and depleted cells. B, Quantification of A. Mean ± SD, n = 15-27 cells pooled from 2 independent experiments. Student's t-test. "
NCBI gene IDs:
"ORP2: 9885"
Figure legend: "C, E, Control and degron ORP2 cells were co-plated, and after 1 day in 5 % LPDS, treated with 50 μg/ml LDL and IAA for the indicated times. Asterisk indicates an  ORP2-depleted cell. Double labeling of intracellular D4H and  Lamp1 (C) or intracellular D4H  and internalized integrin β1 (E). Images were acquired by confocal microscopy. Red box indicates a peripheral region toward the leading edge in the control cell, and blue box a corresponding region in the ORP2-depleted cell."
NCBI gene IDs:
"ORP2: 9885"
Figure legend: "B, Cells stably expressing NPC1-mCherry were treated with control or OCRL siRNAs for 2 days, or transfected with GFP-ORP2 or -ORP2-mHHK (PI(4,5)P2 binding-deficient mutant) for 1 day, stained with  PI(4,5)P2 antibody and imaged by confocal microscopy. For FAK  inhibition, GFP-ORP2 transfected cells were treated with 10 μM PF228 for 4 h. Dashed lines indicate cell edges. Arrowheads indicate colocalization of PI(4,5)P2  and NPC1. C, Quantification of B. Values from Ctrl siRNA and Vector control were pooled and are shown as Ctrl siRNA/Vector ctrl. Mean ± SD, n = 20-43 cells pooled from 2 independent experiments. Student's t-test. "
NCBI gene IDs:
"GFP: 
mCherry: 
NPC1: 4864
OCRL: 4952
ORP2: 9885"
Figure legend: "D, E, Cells stably expressing NPC1-mCherry were transfected with GFP-ORP2 or  GFP-ORP2-mHHK for 1 day and were indicated, treated with 10 μM PF228 for 4 h. For degron  ORP2  cells,  NPC1-mCherry was transiently transfected for 2 days and cells incubated without (-IAA; ORP2 present) or with IAA  (+IAA; ORP2 depleted) for 1 h. Arrowheads indicate tubular NPC1 organelles. Live cell images were acquired by widefield epifluorescence miroscopy and the longest  NPC1 tubule per cell was measured. Mean ± SD, n = 102-122 cells. Students's t-test. Please see also ctrl (MovieEV4) and PF228 treated cell (MovieEV5), both videos 1 min recordings with 1 s frame rate."
NCBI gene IDs:
"GFP: 
mCherry: 
NPC1: 4864
ORP2: 9885"
Figure legend: "A, B, Control and degron ORP2 cells were co-plated, and after 1 day 5 % LPDS, treated with 50 μg/ml LDL and  IAA (+IAA; ORP2 depletion) for the indicated  times and  immunostained for pFAK to quantify its intensity. For rescue experiments, degron ORP2 cells were plated and transfected with GFP-ORP2 or -ORP2-mHHK or -ORP2-∆ELSK for 6 h. Cells expressing GFP-ORP2 constructs at levels similar to endo-GFP-ORP2  were used for quantifying pFAK intensity. Dashed lines indicate cell outlines. See also Fig EV6C. Asterisk indicates cells depleted of endogenous  ORP2. Images were acquired by confocal microscopy. Mean ± SD, n = 20-23 cells pooled from 2 independent experiments. Student's t-test."
NCBI gene IDs:
"GFP: 
ORP2: 9885"
Figure legend: "C, Quantification of FAK protein in cytosolic and membrane fractions in degron ORP2 cells with or without IAA. Cells were starved overnight in LPDS and loaded with 50 µg/mL LDL +/- IAA for 2 h. The proportion of FAK signal in the membrane fraction (of total FAK in cytosol+membranes) is presented ± SD. For no IAA n=13 and for  IAA n= 11, 2 independent experiments. Student's t-test."
NCBI gene IDs:
"ORP2: 9885"
Figure legend: "(B) Schematic of marker-less GFP-tagging at the endogenous locus and representative  live cell  images of Mad1-, Mad2-, and Mad3-GFP   strains (average intensity projections)."
NCBI gene IDs:
"GFP: "
Figure legend: "(C) Representative images of  single molecule mRNA FISH  (smFISH) staining  of  S. pombe using probes against GFP  (red). DNA was stained  with DAPI  (blue). The gamma-value was adjusted to make the cytoplasm  visible; cell shapes are outlined in blue. (D) Frequency distribution of mRNA numbers per cell determined by smFISH; n = number of cells. Curves show fit to a Poisson distribution."
NCBI gene IDs:
"GFP: "
Figure legend: "(E) Frequency distribution of mRNA numbers per cell using FISH  probes against the endogenous genes and using either strains expressing the GFP-tagged gene or the endogenous, untagged gene. Curves show fit to a Poisson distribution. The difference for mad1+ is statistically significant, that for mad2+ is not A lower mRNA number for untagged mad1+ was also observed in an independent strain."
NCBI gene IDs:
"GFP: 
mad1: 2540320
mad2: 2540589"
Figure legend: "(F) Co-staining  by smFISH  using probes against mad1+ and GFP  either in a strain expressing mad1+-GFP  as a positive control or in a strain expressing wild-type mad1+ and mad2+-GFP. Cytoplasmic mad1+ (green) or GFP  mRNA spots (magenta) were quantified as  co-localizing or not with the respective other. For the mad1+-GFP  strain, 544 cells and a total of 1,641 mad1  spots and 1,839 GFP  spots were analyzed; 48 cells were not considered since they did not contain at least one spot of each type in the cytoplasm. For the mad1+ mad2+-GFP  strain, 571 cells and a total of 1,107 mad1 spots and 1,537 GFP  spots were analyzed; 158 cells were not considered since they did not contain at least one spot of each type in the cytoplasm."
NCBI gene IDs:
"GFP: 
mad1: 2540320
mad2: 2540589"