EMBO/SourceData huggingface dataset:
Figure legend: "(A) Immunoblots of protein lysates from ARPE-19 cells infected with either adenovirus expressing Null, TFEB-S211A, or TFE3 upon incubation with DMSO (Control), Tunicamycin (5 µg/ml) or starved in EBSS for 16 h. Data are representative of five independent experiments.(B) Quantification of ATF4, CHOP and GADD34 protein levels in ARPE-19 cells treated as indicated in (A) (mean ± SD of the fold change of the indicated protein to actin ratio from three independent experiments, one-way ANOVA analysis versus the corresponding treatment condition in the adenovirus-Null infected cells, *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001)."
NCBI gene IDs:
"TFE3: 7030
TFEB: 7942"
Figure legend: "(C) Relative Quantitative Real-Time PCR analysis of ATF4, CHOP, GADD34 and MCOLN1 mRNA transcript levels in ARPE-19 cells treated as indicated in (A) (mean ± SD of the RNA fold change of indicated genes normalized to actin mRNA from three independent experiments, one-way ANOVA analysis versus the corresponding treatment condition in the adenovirus-Null infected cells, **p &lt; 0.01, ***p &lt; 0.001)."
NCBI gene IDs:
"actin: 
ATF4: 468
CHOP: 1649
MCOLN1: 57192
GADD34: 23645"
Figure legend: "(A-D) Relative Quantitative Real-Time PCR analysis of ATF4 (A), ASNS (B), CAT1 (C) and xCT (D) mRNA transcript levels in ATF4-depleted ARPE-19 cells infected with either adenovirus expressing Null or TFEB-S211A upon incubation with DMSO (Control), Tunicamycin (5 µg/ml), or starved in EBSS for 16 h (mean ± SD of the RNA fold change of indicated gene mRNAs normalized to actin mRNA from three independent experiments, one-way ANOVA analysis versus the corresponding treatment condition in the adenovirus-Null infected and siRNA Non-Target (siNT) treated cells, *p &lt; 0.05, ***p &lt; 0.001)."
NCBI gene IDs:
"actin: 
ASNS: 440
ATF4: 468
CAT1: 6541
xCT: 23657
TFEB: 7942"
Figure legend: "(E) ChIP-qPCR analysis of the ATF4 promoter and 2000bp upstream of the region of interest (ATF4-Control) from MEF cells that were untreated (control), starved for 2 h, or treated with Tunicamycin (0.1 µg/ml) for 16 h. Amplification regions are indicated by arrows. Chromatin DNA was immunoprecipitated with antibodies for anti-TFE3. Bar graphs show the amount of immunoprecipitated DNA detected by the real-time PCR assay. Values were normalized to the input and plotted as relative enrichment compared to untreated conditions (means ± SD of three independent experiments, students t-test, *p &lt; 0.05, **p &lt; 0.01)."
NCBI gene IDs:
"ATF4: 11911"
Figure legend: "(F) Relative Quantitative Real-Time PCR analysis of SUMF1, DERL1, ATF6, APEX1 and GPX1 mRNA transcript levels in ARPE-19 cells infected with either adenovirus expressing Null or TFEB-S211A (mean ± SD of the RNA fold change of indicated gene mRNAs normalized to actin mRNA from three independent experiments, students t-test, **p &lt; 0.01, ***p &lt; 0.001)."
NCBI gene IDs:
"actin: 
APEX1: 328
ATF6: 22926
DERL1: 79139
GPX1: 2876
SUMF1: 285362
TFEB: 7942"
Figure legend: "(G) Immunoblots of protein lysates from ARPE-19 cells infected with either adenovirus expressing Null or TFEB-S211A. Data are representative of three independent experiments.(H) Quantification of IRE1α and APEX1 protein levels in ARPE-19 cells infected as indicated in (G) (mean ± SD of the fold increase of the indicated protein to actin ratio from three independent experiments, students t-test, ***p &lt; 0.001)."
NCBI gene IDs:
"TFEB: 7942"
Figure legend: "(A) Immunoblots of protein lysates from Null- or TFEB/3 knockout-MEF cells incubated in the presence of DMSO (Control) or Tunicamycin (Tun.) (0.1 µg/ml) for the indicated times. Data are representative of three independent experiments.(B) Quantification of ATF4 protein levels in Null- or TFEB/3 knockout-MEF cells treated as indicated in (A) (mean ± SD of the ATF4 to actin protein ratio from three independent experiments, student's t-test versus the corresponding time point in Null-MEF cells, ns= not significant, **p &lt; 0.01)."
NCBI gene IDs:
"TFEB: 21425"
Figure legend: "(C) Cell viability was determined in Null- or TFEB/3 knockout-MEF cells incubated in the presence of DMSO (Control) or Tunicamycin (Tun.) (0.1 µg/ml) for the indicated times (mean ± SD of the percentage of viable cells compared to control from three independent experiments, one-way ANOVA analysis versus the corresponding time point in Null-MEF cells ***p &lt; 0.001)."
NCBI gene IDs:
"TFEB: 21425"
Figure legend: "(D) Chromatin condensation, an indication of cells undergoing apoptosis, was assessed in Null- or TFEB/3 knockout-MEF cells incubated in the presence of DMSO (Control) or Tunicamycin (0.1 µg/ml) for the indicated times. Confocal microscopy images were obtained from fixed cells stained with Hoechst 33342. Scale bar, 20 µm. Data are representative of three independent experiments."
NCBI gene IDs:
"TFEB: 21425"
Figure legend: "(E) Immunoblots of protein lysates from Null- or TFEB/3 knockout-MEF cells treated as described in (A). Data are representative of three independent experiments.(F-H) Quantification of CHOP (F), PUMA (G) and NOXA (H) protein levels in Null- or TFEB/3 knockout-MEF cells treated as indicated in (E) (mean ± SD of the indicated protein to actin ratio from three independent experiments, student's t-test analysis versus the corresponding time point in Null-MEF cells, ns= not significant, *p &lt; 0.05, **p &lt; 0.01)."
NCBI gene IDs:
"TFEB: 21425"
Figure legend: "(I) adenovirus of protein lysates from ARPE-19 cells infected with either adenovirus expressing Null, TFEB-S211A or TFE3. Data are representative of three independent experiments.(J) Quantification of PUMA protein levels in ARPE-19 cells infected as indicated in (I) (mean ± SD of the fold change of the indicated protein to actin ratio from three independent experiments, one-way ANOVA analysis versus the adenovirus-Null infected cells, *p &lt; 0.05, ***p &lt; 0.001)."
NCBI gene IDs:
"TFE3: 209446
TFEB: 21425"
Figure legend: "(C and D) RNAi of CG34015 in the wing P-compartment significantly reduces the size in both sexes. The images are taken from female wings. Statistical significance was evaluated by Wilcoxon test."
NCBI gene IDs:
"CG34015: 3885570"
Figure legend: "(E) Genome-wide association of CG34015 expression. Manhattan plot shows a strong association of CG34015 RNA with X chromosome in both sexes. P-values are plotted against ordered SNPs along the genome. eQTLs associated with CG34015 are designated as red dots."
NCBI gene IDs:
"CG34015: 3885570"
Figure legend: "(F) Zoomed plot around the CG34015 gene region on X chromosome. eQTLs linked to CG34015 are mostly located upstream of the CG34015 gene region. The red arrow indicates the transcriptional direction."
NCBI gene IDs:
"CG34015: 3885570"
Figure legend: "(G) SNP variation of CG34015-linked eQTLs. SNP states at each eQTL are depicted for each line using different colors (grey dots: reference SNP, blue dots: alternate SNP with a negative effect on RNA level). Lines are ordered from left to right as CG34015 RNA levels increase. Note that the eQTLs are highly linked with each other."
NCBI gene IDs:
"CG34015: 3885570"
Figure legend: "(H and I) Segregation of CG34015 RNA levels and wing size (absCS) by the variation of an eQTL (X_16344746_SNP). P-values are shown. (J) Association of the eQTL (X_16344746_SNP) with population-wide, wing size variation among 143 DGRP lines. P-value and R2 are shown for each sex."
NCBI gene IDs:
"CG34015: 3885570"
Figure legend: "a, Survival curves  of wild type (n=27) and Cdc14  DKO (n=51) mice  during the first 6 weeks of life. (***, p&lt;0.001; Log-rank, Mantel-Cox test). b, Representative images of postnatal day 9 Cdc14  DKO and wild-type littermates. c, Weight  of Cdc14  DKO and wild-type littermates during the first 3 weeks of life (n=4 per genotype). Data information: Bars indicate mean ± SEM. p&gt;0.05; **, p&lt;0.01; ***, p&lt;0.001, Student's t-test with Welch's correction."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "d, Representative macroscopic pictures of brain  dorsal view of wild type (WT) and Cdc14  KO mice  at postnatal 0, 5 and 9 days (P0, P5 and P9). Scale bar, 2 mm. The bar plots show the quantification of brain  width, length and area, respectively. Data information: Bars indicate mean ± SEM. , *, p&gt;0.05; **, p&lt;0.01; ***, p&lt;0.001, Student's t-test with Welch's correction. Non-significant data (p&gt;0.05) are not labeled.In data show the individual data, average and SEM from 3 WT and 3 DKO mice."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "e, Representative cresyl violet staining  images of midsagittal sections of  cerebellar vermis  of WT and Cdc14  KO mice  at P0, P5 and P9. Fissures  underdeveloped in mutant samples are labeled with arrowheads. Scale bar, 700 μm. The bar plots show the quantification of the  cerebellar vermis  area, the number of folia  and the average length of the fissures, respectively. Data information: Bars indicate mean ± SEM. p&gt;0.05; **, p&lt;0.01; ***, p&lt;0.001, Student's t-test with Welch's correction. Non-significant data (p&gt;0.05) are not labeled.In data show the individual data, average and SEM from 3 WT and 3 DKO mice."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "g, Representative images of misdigital sections stained  with DAPI  (blue), Calbindin  (green) and GFAP  (red) of cerebellar vermis of wild-type ad Cdc14  DKO mice  at 9 days after birth. Scale bar, 50 μm. Plots show the quantification of Purkinje cell dendritic  length (average distance of 30 random measurements) and the number of cells per 100 μm from 3 different fields from 3 different mice  in each plot. Data information: Bars indicate mean ± SEM. , *, p&gt;0.05; **, p&lt;0.01; ***, p&lt;0.001, Student's t-test with Welch's correction."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "a. Confocal microscopy  analysis of wild-type and Cdc14  DKO embryonic stem cells (ESCs) subjected to neural differentiation. Neuronal  marker TUJ1  (green), glial  marker GFAP  (red) and nuclear  marker DAPI  (blue) are stained  and quantified after 18 days in differentiation  media. Each dot represents the ratio between TUJ  or GFAP  and DAPI  area in an image containing at least 100 cells from 4 independent experiments with two different clones. **** p-value&lt;0.0001. Student's t-test. Scale bar 50 μm."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "c, Confocal microscopy analysis of WT and Cdc14  DKO ESC  subjected to neural differentiation. Neural progenitor marker Nestin  was stained  and quantified after 6 days in differentiation  media. Each dot represents the ratio between Nestin  and DAPI  area in an image containing at least 100 cells. ****, p&lt;0.0001; Students' t-test."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "d, Western blot  analysis of CDC14B-GFP co-immunoprecipitations. HeLa  cells were transfected with UTF1  and GFP  or CDC14B-GFP. Cytoplasmic  and nuclear  soluble proteins were extracted with RIPA, and nucleolar  and chromatin-bound proteins were extracted with  high salt RIPA. Immunoprecipitations  with anti-GFP  antibody were performed. Total protein extracts and immunoprecipitates  were loaded and UTF  (upper panel) and GFP  (lower panel) were blotted."
NCBI gene IDs:
"GFP: 
CDC14B: 8555
UTF1: 22286"
Figure legend: "e, Immunoprecipitation  of UTF1  upon co-transfection with CDC14B. Total protein was extracted with high-salt RIPA and immunoprecipitations  with anti-UTF1  antibody were performed."
NCBI gene IDs:
"CDC14B: 8555"
Figure legend: "f, Expression  of active CDC14B, but not a phosphatase-dead (PD) isoform, reduces slow-mobility bands of UTF1. Vinculin  was used as a loading control."
NCBI gene IDs:
"CDC14B: 8555"
Figure legend: "a,  Quantitative RT-PCR in wild-type (WT) or Cdc14  DKO ESCs  of the indicated transcripts during neural  differentiation. n=3 independent experiments (±S.E.M.); *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; Student's t-test."
NCBI gene IDs:
"Cdc14: 218294///229776"
Figure legend: "c,  Confocal microscopy  analysis of UTF1  intensity in WT and Cdc14  DKO ESCs  at time  0 (T0) and after 1 day (T1) in differentiation media. The quantification of UTF1  fluorescence mean intensity (FMI) per cell is shown in the plot to the right. Figures and horizontal bars indicate mean ± S.E.M. (n=1269 WT T0 cells, n=2726 WT T1, n=974 Cdc14  DKO T0, n=1173 Cdc14  DKO T1). **, p&lt;0.01; ****, p&lt;0.0001; Student's t-test with Welch's correction."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "d, Western blot analysis of UTF1  expression in WT and Cdc14  DKO ESCs  at time  0 and after 3 days in differentiation media."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "e, Western blot  analysis of UTF1  expression in WT and Cdc14  DKO ESCs  in absence or presence of the kinase  inhibitors roscovitine  (R) or trametinib  (T)."
NCBI gene IDs:
"Cdc14: 229776///218294"
Figure legend: "f, Immunoblot  analysis of UTF1  protein stability after transfection of HeLa  cells with UTF1  and GFP  or CDC14B-GFP. Cells were treated with cycloheximide  (CHX) and total proteins were detected at different time points  as indicated. Cyclin B1  and β-catenin  are used as controls."
NCBI gene IDs:
"GFP: 
CDC14B: 8555
UTF1: 22286"
Figure legend: "g, Western blot  analysis of UTF1  WT or mutants in three (UTF1-3KR) or five (UTF1-5KR) lysines in absence or presence of trametinib  (T) or roscovitine  (R)."
NCBI gene IDs:
"UTF1: 22286"
Figure legend: "h, Immunoblot  of UTF1  mutants in SPOP, SIAH   or SPOP+SIAH   potential binding sites, as well as S48D and S54D phosphorylation sites. Actin  was used as a loading control."
NCBI gene IDs:
"UTF1: 22286"
Figure legend: "Gene-trap-based genetic screen in haploid human  cells for regulators of p38     kinase activation (p38 Thr180/Tyr182 phosphorylation) in response to hyperosmotic shock (500 mM Sorbitol, 1 h). Per gene (dots), the frequency of gene-trap insertions in the 'high' phospho-p38    population divided by the frequency of insertions in the 'low' population is plotted as mutation index (y-axis) against the total number of insertions assigned to the gene (x-axis). Significant negative and positive regulators are colored in orange and blue, respectively (two-sided Fisher's exact test, false discovery rate-corrected, p≤0.05)."
NCBI gene IDs:
"p38: 1432"
Figure legend: "Human U2OS  cells and cells deleted for ZAK  (∆ZAK) were treated with anisomycin  (1 h) or 500 mM sorbitol  (1 h). Lysates were analyzed by immunoblotting as indicated."
NCBI gene IDs:
"ZAK: 51776"
Figure legend: "Unique gene-trap insertions (dots) mapped to the genomic ZAK  locus (x-axis) identified in the low (blue) and high (orange) channel of two individual haploid genetic screens for stress-induced p38  activation and Anisomycin(Brockmann et al., 2017)). The total number of identified insertions was similar for each channel within the individual screens. For visual purposes, insertion dots were spread on the y-axis and exons in the ZAK gene body schematic have been scaled up (compared to introns)."
NCBI gene IDs:
"ZAK: 51776"
Figure legend: "U2OS/S-HA-ZAKβ  cells were induced for expression with doxycycline (DOX) and subjected to the indicated drugs and treatments (1 h). Lysates were analyzed by immunoblotting  with the indicated antibodies. Sorb., sorbitol; Ani., anisomycin; Ars., arsenite."
NCBI gene IDs:
"HA: 
ZAKβ: 51776"
Figure legend: "U2OS  cells stably expressing WT and kinase-dead (KD) versions of S-HA-ZAKβ  were pre-treated with ZAK  inhibitor (ZAKi - 0.5 h) and 500 mM sorbitol  (1 h) as indicated. Whole Cell Extracts (WCE) were analyzed by immunoblotting, and strep-pulldown  material was used in a  kinase assay, separated by SDS-PAGE and analyzed by autoradiography."
NCBI gene IDs:
"HA: 
ZAKβ: 51776"
Figure legend: "U2OS  and ∆ZAK cells rescued with S-HA-ZAKβ, were DOX-induced and treated with 500 mM sorbitol  (1 h) as indicated. Lysates were analyzed by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"HA: 
ZAK: 51776
ZAKβ: 51776"
Figure legend: "U2OS  and ∆ZAK cells were transfected with the indicated siRNAs and treated with sorbitol  (500 mM, 1 h) as indicated. Lysates were analyzed by immunoblotting  with the indicated antibodies. T1, TAK1; M2, MEKK2."
NCBI gene IDs:
"ZAK: 51776
TAK1: 6885
MEKK2: 79109"
Figure legend: "U2OS  cells stably expressing Strep-HA-ZAKβ  or -ZAKα  were treated with sorbitol  (500 mM, 1 h) as indicated. Cells were fixed, immunostained  with HA  antibody and counter-stained  with DAPI. Data information: scale bars, 10 μm."
NCBI gene IDs:
"HA: 
ZAKα: 51776
ZAKβ: 51776"
Figure legend: "U2OS  and ∆ZAK cells stably expressing the indicated Strep-HA-tagged ZAKβ  constructs were treated with sorbitol  (500 mM, 5 min). Lysates were analyzed by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"HA: 
ZAK: 51776
ZAKβ: 51776"
Figure legend: "U2OS  and ∆ZAK  cells were embedded in agarose  and subjected to cyclic compression (0.5 h). Lysates were analyzed by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"ZAK: 51776"
Figure legend: "U2OS  and ∆ZAK  cells stably expressing the indicated Strep-HA-tagged ZAKβ  constructs were embedded in agarose  and subjected to cyclic compression  for 5 or 30 min. Lysates were analyzed as in (B)."
NCBI gene IDs:
"HA: 
ZAK: 51776
ZAKβ: 51776"
Figure legend: "U2OS  and ∆ZAK cells were pretreated with ZAK  inhibitor (ZAKi) for 30 min and subjected to equibiaxial  (Equi) or uniaxial  (Uni) cyclic stretch  (0.5 h) as indicated. Lysates were analyzed as in (B)."
NCBI gene IDs:
"ZAK: 51776"
Figure legend: "U2OS  and ∆ZAK  cells were transfected with the indicated siRNAs and subjected to equibiaxial cyclic stretch (5 min) as indicated. Lysates were analyzed as in (B)."
NCBI gene IDs:
"ZAK: 51776"
Figure legend: "Skeletal muscle (tibialis anterior, TA) and liver  from WT and ZAK-/- mice  were lysed and analyzed for ZAK  isoform expression  by immunoblotting. Ponceau staining of the membrane indicates equal loading."
NCBI gene IDs:
"ZAK: 26409"
Figure legend: "Mouse embryonic fibroblasts (MEF) isolated from WT and ZAK-/- mice  were treated with 500 mM sorbitol  (1 h). Lysates were analyzed by immunoblotting  with the indicated antibodies. *, unspecific band."
NCBI gene IDs:
"ZAK: 26409"
Figure legend: "16-18-week-old WT and ZAK-/- male mice  (n=3 biological replicates) were subjected to the protocol in (D). TA  lysates were analyzed by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"ZAK: 26409"
Figure legend: "C2C12  cells were differentiated into myotubes  for 14 days and transfected with the indicated GFP-ZAKβ   constructs. Cells were fixed and immunostained  with an antibody against α-actinin1. Data information: scale bars, 20 μm."
NCBI gene IDs:
"GFP: 
ZAKβ: 51776"
Figure legend: "16-18-week-old WT and ZAK-/- male mice  were subjected to the experimental protocol in (I). TA muscle  lysates were analyzed by immunoblotting  with the indicated antibodies."
NCBI gene IDs:
"ZAK: 26409"
Figure legend: "H&amp;E staining  of soleus muscle cross sections from 8- and 22-week-old WT and ZAK-/- male mice. Arrows indicate the presence of centralized nuclei. Scale bars, 50 μm. Quantification of (A). Values indicate the percentage of fibers  displaying centralized nuclei and error bars represent the standard deviation (n=3 biological replicates). **, p&lt;0.01 and ***, p&lt;0.001 in t-test with Bonferroni-Dunn correction for multiple comparison."
NCBI gene IDs:
"ZAK: 26409"
Figure legend: "Fluorescent and histological analysis of liver morphology in WT and yap-/- mutant larvae on a lf:CFP background at 5 dpf. The blue colour overlay represents the fluorescent liver tissue. Scale bar: 200μm (bright field, BF) and 50μm (histology, H+E) Quantitative analysis of fluorescent liver area in WT and lf:Yap larvae at 5 dpf. n&gt;30, ****p&lt;0.0001, two-sided Student's t-test; values represent the mean ± standard error of the mean (s.e.m.)"
NCBI gene IDs:
"yap: 561411
Yap: 561411"
Figure legend: "Whole-mount in situ hybridization (WISH) analysis of progenitor marker prox1, hhex, and foxa3 expression in WT and yap-/- mutant embryos at 36 hpf. Arrow and bracket highlight the hepatic bud. Scale bar: 200μm"
NCBI gene IDs:
"foxa3: 30559
hhex: 30098
prox1: 30679
yap: 561411"
Figure legend: "qPCR analysis of hhex and prox1 gene expression in WT and yap-/- mutant embryos at 48 hpf. n&gt;3, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"hhex: 30098
prox1: 30679
yap: 561411"
Figure legend: "WISH analysis of prox1, hhex and foxa3 expression in WT, hs:dnYap and hs:Yap transgenic embryos heat shocked at 24 hpf and fixed at 36 hpf. Arrow and bracket highlight the hepatic bud. Scale bar: 200μm"
NCBI gene IDs:
"foxa3: 30559
hhex: 30098
prox1: 30679
Yap: 561411"
Figure legend: "Quantitative analysis of hepatic bud area (prox1 staining) in WT, hs:dnYap and hs:Yap transgenic embryos heat shocked at 24 hpf and fixed at 36 hpf. n&gt;10, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"Yap: 561411"
Figure legend: "Fluorescent and histological analysis of liver morphology in dissected WT and yap-/- mutant adults on a lf:CFP background. The blue colour overlay represents the fluorescent liver tissue. Scale bar: 2mm (brightfield, BF) and 50μm (histology, H+E)"
NCBI gene IDs:
"yap: 561411"
Figure legend: "Quantitative determination of body mass in WT and yap-/- adults. n=8, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m Quantitative determination of liver size in WT and yap-/- adults as determined by measuring liver mass as a percentage of body mass. n=12, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"yap: 561411"
Figure legend: "qPCR analysis of the hhex expression in adult liver tissue isolated from WT and yap-/- fish. n&gt;3, **p&lt;0.01, two-sided Student's t-test; values represent the mean ± s.e.m qPCR analysis of the prox1 expression in adult liver tissue isolated from WT and yap-/- mutant fish. n&gt;3, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"hhex: 30098
prox1: 30679
yap: 561411"
Figure legend: "Heatmap of RNAseq analysis illustrating statistically significant differential gene expression between WT and yap-/- mutant larvae at 3 dpf. Blue indicates decreased expression, while red demonstrates increased expression"
NCBI gene IDs:
"yap: 561411"
Figure legend: "Heatmap analysis of RNAseq data highlights the reduction of glucose transporters (glut1 and glut2) in yap-/- mutant larvae at 3 dpf"
NCBI gene IDs:
"glut1: 555778
glut2: 322493
yap: 561411"
Figure legend: "qPCR analysis of glut1 and glut2 expression in WT and yap-/- mutant larvae at 3 dpf. n&gt;3, *p&lt;0.05, ***p&lt;0.001, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"glut1: 555778
glut2: 322493
yap: 561411"
Figure legend: "qPCR analysis of glut1 and glut2 expression in WT, hs:dnYap and hs:Yap transgenic embryos heat shocked at 24 hpf and isolated at 30 hpf. n&gt;3, *p&lt;0.05, **p&lt;0.01, ****p&lt;0.0001, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"glut1: 555778
glut2: 322493
Yap: 561411"
Figure legend: "qPCR analysis of glut1 and glut2 expression in liver tissue dissected from WT, yap-/- mutant and lf:Yap transgenic adults. n&gt;3, *p&lt;0.05, **p&lt;0.01, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"glut1: 555778
glut2: 322493
yap: 561411
Yap: 561411"
Figure legend: "Glucose tolerance test in 3-month old WT and yap-/- mutant zebrafish. Blood glucose was measured 0, 30, 60, 120, 180 and 240 min post glucose injection. n&gt;8, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m Glucose tolerance test in 12-month old WT and yap-/- mutant zebrafish. Blood glucose was measured 0, 30, 60, 120, 180 and 240 min post glucose injection. n&gt;8, *p&lt;0.05, two-sided Student's t-test; values represent the mean ± s.e.m"
NCBI gene IDs:
"yap: 561411"
Figure legend: "Clustergram analysis of polar metabolite abundance from WT and yap-/- mutant larvae at 3 dpf as determined by LC-MS/MS via selected reaction monitoring (SRM) analysis. n=4, p&lt;0.05, two-sided Student's t-test"
NCBI gene IDs:
"yap: 561411"