EMBO/SourceData huggingface dataset:
Figure legend: "(E and F) LRRK2 protein levels in AUF1 KO cells with AUF1 expressing back upon AICAr treatment. SH-SY5Y WT and KO cells were transfected AUF1-GFP or GFP and selected with 4μg/ml blasticidin. The resulting stable cell lines were treated with or without 1mM AICAr for 48 hours. LRRK2 expression levels were analyzed by Western blot."
NCBI gene IDs:
"GFP: 
AUF1: 3184"
Figure legend: "(G and H) Overexpressed LRRK2 levels in AUF1 KO cells with AUF1 expressing back upon AICAr treatment. SH-SY5Y WT and AUF1 KO cells were transfected AUF1-GFP or GFP and selected with 4μg/ml blasticidin. The resulting cell lines were transfected with a construct with native LRRK2 cDNA or synthesized LRRK2 cDNA with synonymous codons and treated with or without 1mM AICAr for 48 hours. LRRK2 expression levels were analyzed by Western blot. Images were quantified by ImageJ software."
NCBI gene IDs:
"GFP: 
LRRK2: 
AUF1: 3184
LRRK2: 120892"
Figure legend: "(A and B) AUF1 binds to LRRK2 mRNA upon AICAr treatment. SH-SY5Y WT and AUF1 KO cells were treated with or without 1mM AICAr for 24 hours. RNA and protein complexes were immunoprecipitated using AUF1 antibody. The LRRK2 mRNA levels were analyzed by semiquantitative reverse transcription (RT)-PCR. 18S ribosomal RNA (rRNA) was as a loading control. Images were quantified by ImageJ software. Data are mean ± SEM, n=3 independent experiments. *p &lt; 0.05 by Student's t tests."
NCBI gene IDs:
"AUF1: 3184
LRRK2: 120892"
Figure legend: "(D) Direct interaction of LRRK2 mRNA and four AUF1 isoforms. Biotin-labelled 62-nt LRRK2 RNA probes from native LRRK2 cDNA or synthesized LRRK2 cDNA were incubated with purified AUF1 isoforms in an RNA electrophoretic mobility shift assay (EMSA). Free probes and AUF1 bound probes were detected using a chemiluminescent RNA EMSA kit."
NCBI gene IDs:
"LRRK2: 
LRRK2: 120892"
Figure legend: "(E) LRRK2 mRNA decapping by DCP1A and DCP2. Recombinant DCP1A and DCP2 proteins were purified from HEK 293T cells and incubated with [α-32P]cap-labeled LRRK2 RNA. LRRK2 mRNA decapping was analyzed by one-dimensional thin layer chromatography. The decapping activity is demonstrated by the increased m7GDP levels."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(E and F) LRRK2 protein levels upon the treatment of 1mM AICAr in WT and AMPK KO SH-SY5Ycells for 48 hours. LRRK2 expression levels were analyzed by Western blot. Images were quantified by ImageJ software."
NCBI gene IDs:
"AMPK: 5564///51422///5571///5565///53632///5563///5562"
Figure legend: "(B) Representative confocal images (GFP) of dopamine neurons in each cluster from 9-week-old WT and LRRK2-G2019S transgenic flies with or without 1mM AICAr treatment after posteclosion. Scale bars, 25 μm."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(C-E) Quantification of dopamine neurons per DA cluster in 9-week-old WT and LRRK2-G2019S transgenic flies with or without 1mM AICAr treatment after posteclosion. (F) Total numbers of dopamine neurons in four major DA clusters of 9-week-old WT and LRRK2-G2019S transgenic flies with or without AICAr treatment after posteclosion. Data are mean ± SEM, n=12 flies per group. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.0001 by one-way ANOVA followed by a Tukey's post hoc test."
NCBI gene IDs:
"LRRK2: 120892"
Figure legend: "(A) Representative images of DAneuron staining. WT and LRRK-KO mice were injected with 1ul LPS (5ug/animal) or phosphate buffered saline (PBS) into the substantia nigra pars compacta (SNpc) and administrated with AICAr (2.4μg/day/animal) or saline to same stereotaxic coordinates using osmotic pumps. After 21 days of injection, DA neurons were stained by anti-tyrosine hydroxylase (TH) antibody. Scale bars, 500 μm."
NCBI gene IDs:
"LRRK: 66725"
Figure legend: "(E) CD68 levels in WT and LRRK-KO mice injected with 1μl LPS (5μg/animal) or PBS into striatum followed by administration of AICAr (2.4μg/day/animal) or saline to same stereotaxic coordinates using osmotic pumps. After 21 days of injection, LRRK2 and CD68 expression in striatal tissues near the needle sites was analyzed by Western blot. (F) Quantification of images from (E) using ImageJ software, n=3 animals per group."
NCBI gene IDs:
"LRRK: 66725"
Figure legend: "a, A TRPML1Va-expressing HEK293T cell showed a large inwardly rectifying whole-cell current elicited by voltage steps (from -140 mV to +80 mV in increments of 20 mV) in the standard extracellular (Tyrode's) bath solution. Step duration, 90 ms; holding potential, 0 mV; Vm, membrane potential."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "d, pH-dependence of IFe/TRPML1Va. Whole-cell currents were elicited by repeated voltage ramps (-100 to +100 mV; 400 ms) with a 4-s interval between ramps. Only a portion of the voltage protocol is shown. Holding potential, 0 mV."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "e, Large IFe/TRPML2Va was seen in the presence of 30 and 105 mM Fe2+ (pH 4.6)."
NCBI gene IDs:
"TRPML2: 255231"
Figure legend: "f, Little or no IFe was seen in a TRPML3Va-expressing cell."
NCBI gene IDs:
"TRPML3: 55283"
Figure legend: "a, Current densities (mean ± s.e.m., n = 4-10) of IFe (30 mM Fe2+, pH 4.6) for TRPML1Va and ML4 mutant TRPML1Va channels. Asterisk indicates statistical difference (P &lt; 0.01) compared to TRPML1Va."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "b, 55Fe2+uptake (normalized) in HEK293T cells transfected with vector control, with TRPML1Va, with F408D-TRPML1Va and with T232P-TRPML1Va constructs. Error bars indicate the standard deviation on the basis of two independent triplicate experiments."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "c, [Fe2+]o-dependent quenching of Fura-2 fluorescence in TRPML1Va-transfected cells (arrows), but not in non-transfected control cells (arrowheads) or T232P-TRPML1Va-transfected cells (bottom row). The fluorescence intensity was measured at an excitation wavelength of 360 nm (F360). The original magnification used for all micrographs was ×200."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "d, Average normalized responses of EGFP-positive TRPML1Va-transfected cells (typically n = 20-40 cells) to 1 or 10 mM Fe2+ (pH 4.6)."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "e, No significant quenching was seen in T232P-TRPML1Va-transfected cells."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "f, Slightly less quenching was observed for the F408Dgr;-TRPML1Va-expressing cells."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "c, Lysosomal ITRPML1Va. Switching from lysosome-attached to (lysosome) luminal-side-out configuration significantly reduced the amplitude of the current. The luminal-side-out patch was exposed to the Tyrode's solution. A Cs+-based solution (147 mM Cs-methanesulphonate (Cs-MSA)) was used as a pipette solution for both configurations."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "d, NMDG+-impermeable lysosomal ITRPML1Va was much larger in the absence of divalent cations (nominal divalent-free). e, Lowering pH potentiated lysosomal ITRPML1Va. f, IFe/TRPML1Va induced by 30 mM and 105 mM Fe2+."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "g, Whole-lysosome current in an enlarged lysosome expressing wild-type TRPML1. The pipette (lumen) solution contained nominal divalent-free Tyrode solution. A Cs+-based bath solution (147 mM Cs-MSA) was used. h, Whole-lysosome ITRPML1Va. i, Whole-lysosome IFe/TRPML1. The pipette (lumen) solution contained 105 mM Fe2+ (pH 4.6)."
NCBI gene IDs:
"TRPML1: 57192"
Figure legend: "a, Cultured TRPML1-/- (ML1-/-) skin fibroblasts showed less de-quenching of the iron-sensitive fluorescence than ML1+/- cells did. De-quenching was achieved by preloading the fibroblasts with an iron-sensitive dye, Phen Green SK (PG SK), and then adding the membrane-permeable transition metal chelator, 2,2′-bipyridyl (BPD). 2,2′-BPD is predicted to chelate free cellular iron (also referred to as chelatable or labile iron), which subsequently increases PG SK fluorescence. The original magnification used was ×200"
NCBI gene IDs:
"ML1: 57192
TRPML1: 57192"
Figure legend: "b, The average 2,2′-BPD induced normalized change of fluorescence (Dgr;F/F0) for ML4 cells (ML1-/-; n = 6 experiments) is significantly (asterisk, P &lt; 0.01) lower than for the parental ML1+/- cells. Fibroblast cells (n = 10-20) were analysed for each individual experiment. 4,4′-BPD, a 2,2′-BPD analogue that cannot bind Fe2+, did not induce any significant restoration of PG SK fluorescence. Error bars, s.e.m."
NCBI gene IDs:
"ML1: 57192"
Figure legend: "c, An ML1-/- skin fibroblast cell showed autofluorescence in LAMP1-positive compartments. Autofluorescence (green) was detected within a range of excitation wavelengths (shown with excitation at 480 nm). No significant autofluorescence was observed for a ML1+/+ cell. Lysosomes were stained with a LAMP1 antibody (red). Differential interference contrast (DIC) images are shown for comparison."
NCBI gene IDs:
"ML1: 57192"
Figure legend: "Sexing the established ESCs by detecting the Y-linked Zfy gene using genomic DNA (gDNA)-qPCR, normalized to Gapdh. n.d.: not detected. Average of presumed males was set to 1."
NCBI gene IDs:
"Gapdh: 14433
Zfy: 22768"
Figure legend: "DNA-FISH detection of the number of X-linked Atrx gene foci. Representative images of the DNA-FISH are shown. Nuclei are surrounded by white dotted lines. Magenta: Atrx (white arrowheads), cyan: DAPI. N&gt;100."
NCBI gene IDs:
"Atrx: 22589"
Figure legend: "Nanog HR assay. The percentage of GFP-positive colonies after gene targeting to the Nanog locus is shown. The results for each cell line are shown at right."
NCBI gene IDs:
"GFP: 
Nanog: 71950"
Figure legend: "Nanog expression confirmed by RNA-seq."
NCBI gene IDs:
"Nanog: 71950"
Figure legend: "Detection of the number of X-linked Atrx gene foci by DNA-FISH in GFP-positive subclones of the Nanog-targeting assay (nine independent clones, N&gt; 100 each), performed as described in Figure 1C."
NCBI gene IDs:
"GFP: 
Atrx: 22589
Nanog: 71950"
Figure legend: "Sexing of established ESCs (gDNA-qPCR, Zfy/Gapdh), performed as described in Figure 1B."
NCBI gene IDs:
"Gapdh: 14433
Zfy: 22768"
Figure legend: "Detection of the number of X-linked Atrx gene foci by DNA-FISH with or without dox addition for 96 h (N >100), performed as described in Figure 1C."
NCBI gene IDs:
"Atrx: 22589"
Figure legend: "Detection of the Xist cloud in #B3 by RNA-FISH, with or without dox addition for 96 h ( N &gt;200 each). A representative image is shown [magenta: Xist (white arrowhead), cyan: DAPI. Scale bar: 10 μm]."
NCBI gene IDs:
"Xist: 213742"
Figure legend: "Allele-distinguishable RT-PCR for detecting Hprt, G6pdx, and Rnf12 X-linked genes. Bands with the anticipated sizes expressed from XJF1 or XTX alleles are shown."
NCBI gene IDs:
"G6pdx: 14381
Hprt: 15452
Rnf12: 19820"
Figure legend: "Nanog HR assay. The percentage of GFP-positive colonies after gene targeting to the Nanog locus is shown as described in Figure 2C. #B2 or #B3 were cultured with or without dox addition for 96 h. The combined number of GFP-positive cells and total colonies from all experiments are listed and summarized at right."
NCBI gene IDs:
"GFP: 
Nanog: 71950"
Figure legend: "Detection of the Xist foci by RNA-FISH, with or without dox addition for 96 h (N &gt;200 each). A representative image with percentage of Xist-positive cells is shown [magenta: Xist, cyan: DAPI.]."
NCBI gene IDs:
"Xist: 213742"
Figure legend: "Detection of the number of X-linked Atrx gene foci by DNA-FISH, with or without dox addition for 96 h (N &gt;100 each), performed as described in Figure 1C."
NCBI gene IDs:
"Atrx: 22589"
Figure legend: "Estimated repair events. The flow cytometry plots are shown in Figure EV4. The percentage of GFP-positive cells in TX-DRGFP#11 and TX-EJ5GFP#23 was normalized to the transfection efficiency detected by the VENUS expression vector. Dox (-) was set to 1 and the ratio of relative GFP-positive cells is shown (n=3)."
NCBI gene IDs:
"DRGFP: 
EJ5GFP: 
GFP: 
VENUS: "
Figure legend: "Brcc3 expression confirmed by RNA-seq [biological replicates (n=3) with three different cell lines (male: #A6, #A13, #A17; female: #A4, #A10, #A11)]."
NCBI gene IDs:
"Brcc3: 210766"
Figure legend: "Brcc3 expression confirmed by RT-qPCR [biological replicates (n=3) with three different cell lines (male: #A6, #A13, #A17; female: #A4, #A10, #A11)]."
NCBI gene IDs:
"Brcc3: 210766"
Figure legend: "Confirmation of Brcc3 expression from knockdown samples in by RT-qPCR (technical duplicates)"
NCBI gene IDs:
"Brcc3: 210766"
Figure legend: "Nanog HR assay with Brcc3 knockdown (three biological replicates). The percentage of GFP-positive colonies after gene targeting to the Nanog locus is shown, as shown in Figure 2C. The combined number of GFP-positive cells and total number of colonies are shown (please note that total cell number of #A13 for drug screening was 3.67×107 because of contamination of 1 out of 12 dishes, compared with the other colonies for which 4×107 cells were used)."
NCBI gene IDs:
"GFP: 
Brcc3: 210766
Nanog: 71950"
Figure legend: "Kaplan-Meier curves for the overall survival of breast cancer patients based on BRCC3 (A), XIST (B), and BRCC3/XIST(C) expression levels. HR: hazard ratio."
NCBI gene IDs:
"BRCC3: 210766
XIST: 213742"
Figure legend: "(E) S-phase mock and ZIKV  infected control and Cep63gt/gt MEFs co-stained for Centrin  (green) and flavivirus envelope  (red). (F) Quantification of the percentage of mock or ZIKV-infected Het and Cep63gt/gt MEFs  in S phase with greater than four Centrin  foci. Bars represent the mean ± SD normalized to the control. Asterisk denotes p&lt;0.005 (Student's t test). Three biological replicates are represented. Data information: Scale bars indicate 5μm for all images. All cells were fixed 16 hours post infection (hpi),"
NCBI gene IDs:
"Cep63: 28135"
Figure legend: "(C) We expressed Myc-tagged Brazilian Zika NS3  in 293T/17 cells and immunoprecipitated c-Myc. Precipitated proteins were probed for the Myc  tag of NS3  and endogenous CEP63. The centriole  distal end component, CP110  served as a negative control. Asterisk denotes specific band for CEP63."
NCBI gene IDs:
"Myc: 
NS3: 7751225"
Figure legend: "(G) HeLa  cells transfected with Control or Myc-tagged Brazilian ZIKV NS3  (Br NS3) co-stained for Myc  (red) and Centrin  (green). Data information: Scale bars indicate 5μm for all images."
NCBI gene IDs:
"Myc: 
NS3: 7751225"
Figure legend: "(H) Quantification of the percentage of control and NS3  expressing HeLa  cells with greater than four Centrin  foci. Bars represent the mean ± SD normalized to the control. For all quantifications at least 100 cells were counted per experiment (biological replicates=3). p&lt;0.005 (paired Student's t test) for the comparison of NS3  to mock transfected cells was denoted with asterisk."
NCBI gene IDs:
"NS3: 7751225"
Figure legend: "(D) RT-ddPCR of IFNβ  transcript at the indicated time points in mock and ZIKV-infected H4  cells. Bars represent the mean ± SD normalized to the control. Asterisk denotes p&lt;0.005 (Student's t test). Three biological replicates are represented."
NCBI gene IDs:
"IFNβ: 3456"
Figure legend: "(E) Control and Cep63gt/gt MEFs  were co-stained with antibodies to γ-tubulin (red) and p-Tbk1  (green). Data information: Scale bars indicate 5μm for all images."
NCBI gene IDs:
"Cep63: 28135"
Figure legend: "(F) Immunoblot  analysis of control and Cep63gt/gt MEFs  probed with antibodies to Tbk1  and p-Tbk1. Actin  served as a loading control."
NCBI gene IDs:
"Cep63: 28135"
Figure legend: "(J) SC  and CEP63  siRNA treated HeLa  cells were  co-stained for Centrin  (red) and DTX4  (green). Data information: Scale bars indicate 5μm for all images."
NCBI gene IDs:
"CEP63: 80254"
Figure legend: "(K) TBK1  or c-Myc (negative control) were immunoprecipitated  from Control or Cep63 mutant MEFs. Precipitating proteins were probed with antibodies to TBK1  and K48-linked ubiquitin."
NCBI gene IDs:
"Cep63: 28135"
Figure legend: "M2-induced human monocyte-derived macrophages were treated with 100 nM of the indicated drug either continuously for 48h, or initially for 2h in the presence or absence of FA-glucosamine (competition) followed by 46h in the absence of drug (2+46h), as described in Fig 1. mRNA levels of profibrotic markers, Arg1 (A), CD206 (B) and CD163 (C) Changes in both sets of cytokines were inhibited by blockade of unoccupied folate receptors with excess FA-glucosamine (2+46h, competition)."
NCBI gene IDs:
"Arg1: 383
CD163: 9332
CD206: 4360"
Figure legend: "BALF was collected on day 21 and centrifuged to isolate cell pellets. Cells (primarily macrophages) were analyzed by qPCR for Arg1 (C), MMP9 (D), TIMP3 (E)"
NCBI gene IDs:
"Arg1: 11846
MMP9: 17395
TIMP3: 21859"
Figure legend: "BALF was collected on day 21 and centrifuged to isolate cell pellets. Cells (primarily macrophages) were analyzed by qPCR for CD86 (F) and IRAK4 (G) (n=5)."
NCBI gene IDs:
"CD86: 12524
IRAK4: 266632"
Figure legend: "(E) Representative photos of  iWAT,  eWAT  and BAT from  Ftoflox/flox and FtoAKO mice fed a HFD. Scale bar, 1 cm. (F) Representative H&amp;E staining  of iWAT, eWAT  and BAT from  Ftoflox/flox and  FtoAKO mice fed a HFD. Scale bar, 200 μm. ( "
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(I-K) Serum  levels of glucose (I), triglyceride (J) and free fatty acid (FFA) (K) in Ftoflox/flox and FtoAKO mice  fed a  HFD followed by fasting. Data information: The data were presented as the mean ± SD (n = 6). Statistical analyses were performed using Student t‐test. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(A)  O2  consumption of 16-week-old Ftoflox/flox and FtoAKO mice fed a HFD. White and gray areas in the graphs indicate  day  and night, respectively. (B)  CO2  generation of 16-week-old  Ftoflox/flox and FtoAKO mice fed a HFD. White and gray areas in the graphs indicate day and  night, respectively. ( Data information: The data were presented as the mean ± SD of n = 6 mice per group or triplicate tests (n = 3). Statistical analyses were performed using Student t‐test. ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(F)  qPCR  analysis of genes involved in thermogenesis,  mitochondrial  biogenesis and adipogenesis in iWAT of Ftoflox/flox and  FtoAKO mice."
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(G)  Western blot  analysis of the protein levels of UCP1, PPARGC1A and  PPARG in iWAT of Ftoflox/flox and FtoAKO mice."
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(I) Immunofluorescence detection of  UCP1 in iWAT from Ftoflox/flox and FtoAKO mice following 6 days cold exposure (4°C). Scale bar, 20 μm."
NCBI gene IDs:
"Fto: 26383"
Figure legend: "(C) The  O2 consumption rate (OCR) in differentiated beige adipocytes from  3T3-L1 cells overexpressing Vec or HIF1A  plasmid. The average basal and maximal respiration rates are shown in right panel. Data information: The data were presented as the mean ± SD of triplicate tests (n = 3). Statistical analyses were performed using Student t‐test. ∗p &lt; 0.05, ∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"HIF1A: 15251"
Figure legend: "(C) Distribution of m6A peaks in  Hif1a mRNA transcript in siCtrl and siFto 3T3-L1 cells. Blue boxes represent exons, and blue lines represent introns. The m6A  peaks were in the dashed red box."
NCBI gene IDs:
"Fto: 26383
Hif1a: 15251"
Figure legend: "(D) Methylated RNA immunoprecipitation  (MeRIP)-qPCR analysis of  m6A levels of Hif1a mRNA in siCtrl and siFto 3T3-L1 cells. Data information: The data were presented as the mean ± SD of triplicate tests (n = 3). Statistical analyses were performed using Student t‐test.∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001."
NCBI gene IDs:
"Fto: 26383
Hif1a: 15251"
Figure legend: "(H) Western blot  analysis of the protein levels of HIF1A, UCP1,  PPARGC1A, PPARG and GFP  in  3T3-L1 cells infected with  lentiviruses pCDHpuro expressing Vec, WT- or MUT-HIF1A."
NCBI gene IDs:
"HIF1A: 15251"
Figure legend: "(A) Western blot analysis of the protein levels of  YTHDF1, YTHDF2  and  HIF1A in siCtrl, siYthdf1 and siYthdf2 3T3-L1  cells."
NCBI gene IDs:
"Ythdf1: 228994
Ythdf2: 213541"
Figure legend: "(G)  Polysome  profiling of 3T3-L1 cells overexpressing Vec or YTHDC2 plasmid. (H) qPCR  analysis of mRNA levels of Hif1a in  40-80S or polysome fraction in 3T3-L1  cells overexpressing Vec or YTHDC2  plasmid. (I)  Polysome  profiling  of siCtrl and siYthdc2 3T3-L1 cells. (J) qPCR analysis of mRNA levels of Hif1a in 40-80S or  polysome fraction in siCtrl and siYthdc2 3T3-L1  cells. Data information: The data were presented as the mean ± SD of triplicate tests (n = 3). Statistical analyses were performed using Student t‐test.∗∗p &lt; 0.01, ∗∗∗p &lt; 0.001. "
NCBI gene IDs:
"Hif1a: 15251
YTHDC2: 240255
Ythdc2: 240255"
Figure legend: "B. Western blot analysis of RAB6A/A' and RAB6B  protein levels in WT, Emx1-Cre; RAB6loxP/loxP (RAB6A  KO), RAB6B-/- (RAB6B  KO) and Emx1-Cre; RAB6AloxP/loxP; RAB6B-/- (RAB6A/B dKO) E15.5 cortical  extracts."
NCBI gene IDs:
"Cre: 2777477
Emx1: 13796
RAB6A: 19346
RAB6B: 270192"
Figure legend: "A. PAX6 staining  in WT and RAB6A/B dKO E15.5 brains. Scale bar = 50µm. B. Percentage of PAX6+ cells located in the upper half of the cortex  of WT and RAB6A/B dKO E15.5 brains. WT: 4282 cells from N=6 brains. RAB6A/B dKO: 1241 cells from N=3 brains. Mann-Whitney U test. Data information: All error bars indicate SD."
NCBI gene IDs:
"RAB6A: 19346"
Figure legend: "C. Phospho-Vimentin  (p-Vim) staining  in WT and RAB6A/B dKO E15.5 brains. Scale bar = 50µm. D. Percentage p-VIM+ cells dividing ectopically, in the upper half of the cortex  of WT and RAB6A/B dKO E15.5 brains. WT: 1713 cells from N=6 brains. RAB6A/B dKO: 506 cells from N=3 brains. Mann-Whitney U test. Data information: All error bars indicate SD."
NCBI gene IDs:
"RAB6A: 19346"
Figure legend: "G. Electroporation of  RAB6AloxP/loxP; RAB6B-/- E14.5 embryos  with mCherry  (control) or mCherry  + CRE  (RAB6A/B dKO) and live imaging of delamination events at E17.5. H. Apical endfoot detachment and retraction events during 20 hours movies in mCherry   or mCherry  + CRE  electroporated cells at E17.5. mCherry: N=69 cells from 5 movies. mCherry  + CRE: N=52 cells from 4 movies. Fisher's exact test, *p ≤ 0.05. Data information: All error bars indicate SD."
NCBI gene IDs:
"mCherry: 
CRE: 2777477
RAB6A: 19346
RAB6B: 270192"
Figure legend: "A. Localization  of the Golgi apparatus  (ManII-GFP) and the centrosome  (𝛾-tubulin) in E15.5 mCherry-electroporated radial glial cell. The Golgi apparatus is localized  basally, away from the centrosome. Scale bar = 5µm. B. Average distance between the apical-most part of the  Golgi apparatus and the apical surface in aRG cells. N=224 cells from 3 independent brains."
NCBI gene IDs:
"mCherry: "
Figure legend: "I Live imaging  of GFP-RAB6A  in control, dynarrestin-treated and CC1-p150-expressing  aRG cells at E15.5. Scale bars = 5µm. Distance = 5µm, time  = 30 seconds. J. Number of RAB6A+ vesicles  in the apical  process of DMSO  and dynarrestin-treated mouse aRG cells. K. Relative time  spent by RAB6A+ vesicles  in apical  movement phase, in DMSO  and dynarrestin-treated mouse aRG cells. L. Number of RAB6A+ vesicles  in the apical  process of mCherry  and CC1-p150-expressing  aRG cells. M. Relative time  spent by RAB6A+ vesicles  in apical  movement phase, in mCherry  and CC1-p150-expressing aRG cells. DMSO  treatment slightly affected RAB6A  dynamics, as compared to mCherry  control. Data information: (J, K, L, M) 216 vesicles  from N=11 cells for DMSO, 145 vesicles  from N=25 cells for dynarrestin, 173 vesicles  from N=17 cells for mCherry  control, 71 vesicles  from N=15 cells for CC1-p150. Mann-Whitney U test *p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001. All boxplots: whiskers indicate min and max, boxes indicate 25th and 75th percentile, and central band indicates the median."
NCBI gene IDs:
"mCherry: 
p150: 1639"