EMBO/SourceData huggingface dataset:
Figure legend: "K, Tumor growth after subcutaneous injection of 2.0x106 A375 cells transduced with pBABE-JUNB (JUNB) or pBABE empty vector (EV) retrovirus (n=10 mice per group). Results are expressed as mean ±SEM. Repeated-measures ANOVA was used for the statistical test of tumor growth. L, Tumor weight after the subcutaneous injection of A375 cells as shown in (K). Tumor weight was analyzed 20 days after subcutaneous injection. Results are expressed as mean ±SEM. The two-sided Mann-Whitney U-test was used for the statistical test of tumor weight."
NCBI gene IDs:
"JUNB: 3726"
Figure legend: "J: qPCR analysis of IL-6 in msEGCs that were treated for 6h with ATP."
NCBI gene IDs:
"IL-6: 16193"
Figure legend: "D: P2X2 antagonism of ATP induced mRNA expression of IL-6, GFAP and RCAN by qPCR in msEGCs. Cells were treated with the P2X2-antagonist PSB-1011 (20µM) alone or together with ATP (10µM) for 6h."
NCBI gene IDs:
"GFAP: 14580
IL-6: 16193
RCAN: 54720"
Figure legend: "F: P2X2-siRNA reduces P2X2-mRNA and dampens the gliosis gene expression after ATPɣS (100µM) treatment for 6h."
NCBI gene IDs:
"P2X2: 231602"
Figure legend: "G: P2X2-siRNA reduces IL-6 release after ATPɣS treatment (10µM, 100µM) for 6h."
NCBI gene IDs:
"P2X2: 231602"
Figure legend: "I: PSB-1011 (20μM) treatment inhibited ATP-triggered calcium responses in HEK cells transfected with P2X2. Data are represented as ΔF/F0 +SEM; n=102 HEK cells. J: The P2X2 receptor antagonist PSB-0711 (20μM) nearly abolished the ATP-triggered calcium response in HEK cells transfected with P2X2. n=219 HEK cells."
NCBI gene IDs:
"P2X2: 22953"
Figure legend: "(G) Human ILC2 sorted from  peripheral blood   and in vitro expanded were transduced with Luc-sh or Maf-sh as in (C). hILC2s were then stimulated for 48h with IL-33 and the concentration of the indicated cytokines in the supernatants was measured (Multiple Mann-Whitney tests, *p&lt;0.05, **p&lt;0.01) Bars represent mean ± SEM. Data information: Each dot represents data from one individual mouse or from one donor."
NCBI gene IDs:
"Luc: 
Maf: 4094"
Figure legend: "(E) ChIP-qPCR  analysis of c-Maf occupancy on IL-4,  IL-5  and IL-13 promoters in untreated mouse  and human ILC2s (IL-2 stimulation) and in mouse  and human ILC2s  treated with  IL-33 (n=3 biological replicates)."
NCBI gene IDs:
"IL-13: 3596
IL-4: 3565
IL-5: 3567"
Figure legend: "(E, F) c-Maf  quantification by qPCR (E) and cytokine concentration (F) in the supernatants of adult and cord blood ILC2s  unstimulated, stimulated for 3h with PMA-Ionomycin  (3h stim) and stimulated for an overnight with  IL-33 and then 3h with PMA-Ionomycin  (o.n. stim). ILC2s  were sorted from 3 different donors. (E) Multiple T tests, unstimulated: p=0.000101; 3h stim: p=0.0036. (F) Multiple T tests, IL-13: p=0.022;  IL-5: p=0.015 (*p&lt;0.05). Bars represent mean ± SEM."
NCBI gene IDs:
"c-Maf: 4094"
Figure legend: "(A) Representative images of COS7 cells expressing GFP-COP1 (green) co-transfected with empty vector or the indicated TRIB1-FLAG construct (red). Cells were stained with anti-FLAG (Sigma) and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm. See also Appendix Fig S1B for western blots showing the expression levels of the constructs used."
NCBI gene IDs:
"FLAG: 
TRIB1: 10221"
Figure legend: "(B) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 in (A). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP-COP1 with empty vector was calculated using the Student t test (****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(A) Representative images of COS7 cells expressing COP1-3xFLAG (green) co-transfected with empty vector, GFP, or the indicated GFP-TRIB1 constructs (red). Cells were stained with anti-FLAG (Sigma) and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"GFP: 
TRIB1: 10221"
Figure legend: "(B) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of COP1-3xFLAG in (A). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP-COP1 with empty vector was calculated using the Student t test (*p &lt; 0.01; **p &lt; 0.001; ***p &lt; 0.0001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(C) Representative images of COS7 cells expressing GFP-COP1 (green) co-transfected with empty vector, TRIB1-FLAG, TRIB2-FLAG, or TRIB3-FLAG (red). Cells were stained with anti-FLAG (Sigma) and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"FLAG: 
TRIB1: 10221
TRIB2: 28951
TRIB3: 57761"
Figure legend: "(D) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 in (C). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP-COP1 with empty vector was calculated using the Student t test (ns, not significant; ***p &lt; 0.0001; ****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(B) Coimmunoprecipitation of COP1 WD40 mutants with TRIB1 from COS7 cells transiently transfected with the indicated GFP-COP1 constructs and untagged TRIB1."
NCBI gene IDs:
"GFP: 
COP1: 64326
TRIB1: 10221"
Figure legend: "(C) Representative images of COS7 cells transiently transfected with the indicated GFP-COP1 constructs (green). Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(D) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 constructs in (C). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP-COP1 WT was calculated using the Student t test (***p &lt; 0.0001; ****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(B) Representative images of COS7 cells transiently transfected with GFP-COP1 WT or GFP-COP1 4A (green). Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(C) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 in (B). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance relative to GFP-COP1 WT was calculated using the Student t test (****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(F) Representative images of COS7 cells transiently transfected with the indicated GFP-COP1 constructs (green) and empty vector or TRIB1-FLAG (red). Cells were stained with anti-FLAG (Sigma) and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"FLAG: 
GFP: 
COP1: 64326
TRIB1: 10221"
Figure legend: "(G) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 constructs in (F). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance was calculated using the Student t test (ns, not significant, ***p &lt; 0.0001; ****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(A) Representative images of COS7 cells transiently transfected with GFP-COP1 WT or GFP-COP1 NESmut (V238A/L242A) (green) and empty vector or 3xFLAG-CRM1 (grayscale). Cells were stained with anti-FLAG (Cell Signaling) and anti-rabbit Alexa Fluor 680. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"FLAG: 
GFP: 
COP1: 64326
CRM1: 7514"
Figure legend: "(B) Representative images of COS7 cells transfected with GFP-COP1 WT (green), the indicated TRIB1-HA constructs (red), and empty vector or 3xFLAG-CRM1 (grayscale). Cells were stained with anti-FLAG (Cell Signaling), anti-HA (Sigma), anti-rabbit Alexa Fluor 680, and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"FLAG: 
GFP: 
HA: 
COP1: 64326
TRIB1: 10221
CRM1: 7514"
Figure legend: "(C) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 constructs in (A) and (B). Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance was calculated using the Student t test (ns, not significant; *p &lt; 0.01; ****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(E) Representative images of COS7 cells transiently transfected with the indicated GFP-COP1 constructs (green) and 3xFLAG-CRM1 (grayscale). Cells were stained with anti-FLAG (Sigma) and anti-mouse Alexa Fluor 568. Nuclei were counterstained with DAPI (blue). Scale bar, 10 µm."
NCBI gene IDs:
"FLAG: 
GFP: 
COP1: 64326
CRM1: 7514"
Figure legend: "(F) Quantification of the average ratio of nuclear/cytoplasmic fluorescence of GFP-COP1 constructs in (E) and Figure EV6C. Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed. Significance was calculated using the Student t test (ns, not significant; ****p &lt; 0.00001)."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(G) Quantification of percentage of cells observed in (E) and Figure EV6C with the indicated GFP-COP1 construct exhibiting nuclear (Nuc), nuclear and cytoplasmic (Nuc/Cyt), or cytoplasmic (Cyt) localization. Mean values ± S.E.M. are shown for three independent experiments where 50 individual cells per experiment were analyzed."
NCBI gene IDs:
"GFP: 
COP1: 64326"
Figure legend: "(B) Genotyping of Brd4fl/fl mice with ERT2-Cre (Cre) or without ERT2 -Cre (+/+). Tamoxifen treatment deleted 2.2 kb Brd4fl/fl fragment from Cre/+ and Cre/Cre mice (top). Cre/+, Cre/Cre and +/+ mice were confirmed by the presence of ERT2 -Cre band (Cre), or its absence (WT) (bottom)."
NCBI gene IDs:
"Brd4: 57261
Cre: 2777477
ERT2: 2099"
Figure legend: "(C) WT and Brd4 KO cells were tested for mRNA (left) and the protein (right) by qRT-PCR and immune blot assays. Values represent the average of three experiments +/- SD."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "(D) Vav-cre mediated Brd4 depletion was verified for cells from Brd4fl/fl Vav-Cre mice (fetal liver or spleen) by qRT-PCR and Immunoblot (left and right). Values represent the average of three experiments +/- SD."
NCBI gene IDs:
"Brd4: 57261
cre: 2777477
Cre: 2777477
Vav: 22324"
Figure legend: "(A) Flow cytometry profiles of peritoneal cells from WT and KO mice stained for CD19 and F4/80 (left). FACS profile of CD19- subset showing CD11b+ and F4/80+ subsets of cells from WT and KO macrophage populations (middle). Cell numbers (right) represent the average of 4 WT and 4 Brd4 KO mice +/- S.D. Note a reduction in F4/80+ cells from Brd4 KO peritoneum."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "(B) Flow cytometry profiles of peritoneal cells from IL-4C injected WT and KO mice (left). Cell numbers (right) represent the average of 4 WT and 4 Brd4 KO mice +/- S.D."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "(D) Microarray analysis of macrophage gene expression in IL-4C treated WT or Brd4 KO mice. Macrophages were isolated from peritoneal cells by the MACS system. Differentially expressed genes were identified with a cut-off line of log 2fold change &gt;2 difference with p value of &lt; 0.05. Red dots indicate differentially expressed genes (KO vs WT) and gray dots are those below the cut-off line."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "(E) qRT-PCR analysis of genes representative of the BRD4 independent group (unaffected by KO), dependent group (downregulated by KO) or upregulated by KO. Data were obtained from three independent macrophage samples. Values are the average of 3+/- S.D."
NCBI gene IDs:
"BRD4: 57261"
Figure legend: "(E) Binding of BRD4 and PolII and distribution of histone marks (H3K27ac and H3K9ac) on BRD4 dependent and independent genes (from -5kb of TSS to +5kb of transcription end site (TES)) in UT and LPS treated WT and KO macrophages. Normalized ChIP-seq signals (rpm/bp) are shown on the Y axis. Arrows show reduced signals in Brd4 KO macrophages."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "(F) Gene tracks of normalized island-filtered ChIP-seq peaks for BRD4, Pol II, and indicated histone modification marks, along with RNA-seq peaks for a BRD4 independent gene (Ccl9) and a BRD4 dependent gene (Fcgr2b). Values on the Y axis are normalized ChIP-seq signals (rpm/bp)."
NCBI gene IDs:
"BRD4: 57261
Ccl9: 20308
Fcgr2b: 14130"
Figure legend: "(C) Examples of nearest genes that gained or lost SEs upon LPS treatment. Gene tracks for gained and lost loci (near Tnf and Csf1r) showing clusters of BRD4, Pol II and H3K27ac signals. Red bar represents SE length."
NCBI gene IDs:
"Csf1r: 12978
Tnf: 21926"
Figure legend: "(C) Gene tracks of BRD4 and p65 ChIP-seq peaks and RNA-seq profiles on a BRD4 independent gene (Il1b), a gene upregulated in Brd4KO macrophages (Ccl7), and a BRD4 dependent gene (Ptgs2)."
NCBI gene IDs:
"Brd4: 57261
Ccl7: 20306
Il1b: 16176
Ptgs2: 19225"
Figure legend: "(D) Enhancers ranked by increasing p65 signals in WT (left) and Brd4 KO macrophages (right)."
NCBI gene IDs:
"Brd4: 57261"
Figure legend: "A. NLK but not kinase-negative NLK (Flag-NLK-KM; K155M) caused mobility shift of EGFP-YAP. EGFP-YAP was transfected with empty vector, Flag-NLK-WT, or Flag-NLK-KM into HEK293T cells. Mobility shift was shown by immunoblotting."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "B. NLK phosphorylates YAP in vitro. Flag-NLK-WT, or Flag-NLK-KM was immunoprecipitated with anti-Flag antibody from cell lysates of HEK293T cells transfected with each plasmid and subjected to in vitro kinase assay with bacterially purified GST-YAP in the presence of [32P-ATP]. Phosphorylation of GST-YAP and autophosphorylation of NLK were examined by autoradiography (top panel), and Coomassie Blue staining showed similar levels of GST-YAP and Flag-NLK (bottom panel)."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "B. NLK-WT but not NLK-KM reduces Ser127 phosphorylation of endogenous YAP. Cell lysates from HEK293 cells transfected with empty vector (-), Flag-NLK-WT, or Flag-NLK-KM were immunoblotted with antibodies shown in the figure (left panel). The ratio of p-YAP(S127) or p-YAP(S397)/total-YAP of three independent western blots was quantified (right panel). Data are presented as mean ± sem. **P &lt; 0.01. Student t-test was used for statistical analysis."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "C. Deficiency of NLK leads to increased YAP phosphorylation at Ser127. Lysates from wild type and two independent NLK knockout HEK293 cells were immunoblotted with antibodies indicated in the figure (left panel). The ratio of p-YAP(S127) or p-YAP(S397)/total-YAP of three independent western blots was quantified (right panel). Data are presented as mean ± sem. *P &lt; 0.05 and **P &lt; 0.01. Student t-test was used for statistical analysis."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "D. Ectopic expression of NLK attenuates YAP phosphorylation at Ser127 while increases phosphorylation at Ser128. Cell lysates from HEK293 cells transfected with empty vector (-), Flag-NLK-WT, or Flag-NLK-KM were immunoblotted with antibodies shown in the figure."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "E. Substitution of Ser127 with alanine enhances phosphorylation at Ser128, and vice versa. Cell lysates from HEK293T cells transfected with empty vector (-), EGFP-YAP, EGFP-YAP-S127A, or EGFP-YAP-S128A were immunoblotted with antibodies shown in the figure."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "A. Ectopic expression of NLK attenuates interaction between YAP and 14-3-3. Lysates from HEK293T cells transfected with plasmids indicated in the figure were immunoprecipitated with anti-HA antibody and immunoblotted with anti-GFP antibody (top two panels). Whole cell lysates (WCL) were immunoblotted with antibodies shown in the figure (bottom three panels)."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "B. Deficiency of NLK leads to increased interaction between YAP and 14-3-3. Lysates from NLK knockout HEK293 cells were immunoprecipitated with anti-YAP antibody and immunoblotted with anti-14-3-3 antibody (top two panels). WCL were immunoblotted with antibodies indicated in the figure (bottom three panels)."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "D. Phosphorylation status of Ser127 or Ser128 determines subcellular localization of YAP. Immunofluorescence analysis was performed in HeLa cells transfected with EGFP-YAP, EGFP-YAP-S127A, EGFP-YAP-S128A, or EGFP-YAP-S128D at high cell density. Figure shows a representative of multiple areas. Nuclei were stained with Hoechst 33342. Scale bars: 20 μm."
NCBI gene IDs:
"YAP: 10413"
Figure legend: "E. NLK knockout leads to increased cytoplasmic localization of endogenous YAP. Immunoblot analysis shows significant reduction of NLK level in NLK knockout HEK293 cell pools (top panel)."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "E. NLK knockout leads to increased cytoplasmic localization of endogenous YAP. To determine subcellular localization of YAP indirect immunofluorescence analysis was performed in NLK knockout HEK293 cell pools (bottom panel). Figure shows a representative of multiple areas. Nuclei were stained with DAPI. Scale bars: 20 μm."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "C. Level of endogenous NLK mRNA is unaffected, whereas the expression of YAP target genes was reduced, at high cell density. Quantitative real-time PCR analyses for expression of AMOTL2, CTGF, CYR61, and NLK in low (LD) and high density (HD) NIH3T3 cells were performed. Quantification of AMOTL2, CTGF, CYR61, and NLK mRNA was normalized with the level of Gapdh. Data represent average values from a representative of multiple experiments performed in triplicate. Error bars indicate standard deviations of triplicate measurements. Data are presented as mean ± sem. *P &lt; 0.05 and ***P &lt; 0.005. Student t-test was used for statistical analysis."
NCBI gene IDs:
"AMOTL2: 56332
CTGF: 14219
CYR61: 16007
NLK: 18099"
Figure legend: "A. Knockout of NLK reduces the interaction between endogenous YAP and TEAD. Lysates from NLK knockout HEK293 cells were immunoprecipitated with anti-TEAD1 antibody and immunoblotted with anti-TEAD1 antibody or anti-YAP antibody (top panel). Whole cell lysates (WCL) were immunoblotted with antibodies indicated in the figure."
NCBI gene IDs:
"NLK: 51701"
Figure legend: "C. Downregulation of nemo with RNAi achieved in the posterior compartment of wing imaginal discs (co-expression of GFP serves to mark the posterior compartment) using the hh-Gal4 driver results in decreased expression of the Yorkie target gene DIAP1. Two different RNAi constructs produce similar effect. Panels below show Z-stacks of magnified areas around the A/P border (left). Quantification of DIAP1 levels achieved upon posterior-downregulation of nemo, measured as normalized posterior-to-anterior levels and compared to similar expression of GFP. Data are present as mean ± sem, n=8 discs. *P &lt; 0.05. Student t-test was used for statistical analysis (right). Scale bars: 50 µm."
NCBI gene IDs:
"hh: 42737
nemo: 38890"
Figure legend: "D. Yorkie is epistatic to Nemo: co-overexpression of the constitutively active mutant yki[S168A] together with nmo-RNAi produces typical yki[S168A] phenotypes: severe overgrowth of the affected area with concomitant increase in yki target gene expression (DIAP1). Due to lethality of the hh-Gal4-driven co-overexpression, another posterior driver en-Gal4 was used instead; anti-Hh staining (green) was used to mark the posterior compartment (left panel). Quantification posterior DIAP1 levels in UAS-RNAi-nemo; en-Gal4 and UAS-yki[S168A]; en-Gal4, presented as in (C); n= 3-6 discs. Posterior DIAP1 levels in UAS-RNAi-nemo; en-Gal4 are also significantly different from control UAS-GFP; en-Gal4 discs (right panel). Data are presented as mean ± sem. ***P &lt; 0.005. Student t-test was used for statistical analysis (right). Scale bars: 50 µm."
NCBI gene IDs:
"en: 36240
hh: 42737
nemo: 38890
nmo: 38890
yki: 37851"
Figure legend: "E. Mutant forms of YAP which have different phosphorylation status of Ser127 or S128 differentially rescue retarded wound healing caused by NLK knockout. HEK293 wild type or HEK293 NLK knockout cells were transfected with indicated plasmids (\"EV\" stands for \"empty vector\"), and the wounded areas after 0 and 24 hr were measured by using the TScratch program (left panel). Graphs show percentage of unfilled areas at 24 hr compared to 0 hr, and average values from a representative of multiple experiments performed in triplicate (right-top panel)."
NCBI gene IDs:
"NLK: 51701
YAP: 10413"
Figure legend: "E. Mutant forms of YAP which have different phosphorylation status of Ser127 or S128 differentially rescue retarded wound healing caused by NLK knockout. HEK293 wild type or HEK293 NLK knockout cells were transfected with indicated plasmids (\"EV\" stands for \"empty vector\"), and the wounded areas after 0 and 24 hr were measured by using the TScratch program (left panel). Graphs show percentage of unfilled areas at 24 hr compared to 0 hr, and average values from a representative of multiple experiments performed in triplicate (right-top panel). Lysates were immunoblotted with antibodies indicated in the figure (right-bottom panel). Error bars indicate standard deviations of triplicate measurements. Data are presented as mean ± sem. *P &lt; 0.05. Student t-test was used for statistical analysis (right). Scale bars: 200 μm."
NCBI gene IDs:
"NLK: 51701
YAP: 10413"
Figure legend: " (B) When transposon insertion libraries subjected to ampicillin resistance selection, a substantial increase in transposon insertion frequency in the pstSCA genes was observed in strains containing the protein folding sensor (pBR322 bla::Im7 L53A I54A) relative to a strain containing just pBR322. Transposon insertion frequency increased 21-, 24-, and 8-fold in the pstS, pstA, and pstC genes, respectively, compared to the insertion frequency found with no ampicillin selection. "
NCBI gene IDs:
"Im7: 
bla: 59687646///59688953///59682938///59688725///59687632
pstA: 948239
pstC: 948238
pstS: 948237"
Figure legend: " (A) To verify that disruption of the pst genes is sufficient to increase ampicillin resistance of strains containing the pBR322 bla::Im7 L53A I54A folding biosensor and to test which of the genes in the pst operon are sufficient for this effect, pBR322 and pBR322 bla::Im7 L53A I54A were transformed into strains containing in-frame deletion mutants of each of the pst genes (pstS, pstC, and pstA, respectively). The deletion mutants of each of these pst genes showed higher ampicillin resistance than did the WT strain MG1655 in the presence of pBR322 bla::Im7 L53A I54A, but did not shower higher ampicillin resistance in the presence of pBR322. "
NCBI gene IDs:
"Im7: 
bla: 59687646///59688953///59687632///59688725///59682938
pstA: 948239
pstC: 948238
pstS: 948237"
Figure legend: " (B) Levels of β-lactamase (Bla) and β-lactamase-Im7 L53A I54A were detected by Western blotting using a polyclonal antibody recognizing β-lactamase in the WT strain MG1655 and in isogenic strains containing insertions in the pstSCA genes (CL248 (pstS::Tn5), CL249 (pstC::Tn5), CL250 (pstA::Tn5)). Maltose binding protein (MBP) was used as a control. "
NCBI gene IDs:
"β-lactamase: 59687632
pstA: 948239
pstC: 948238
pstS: 948237"
Figure legend: " (C) β-lactamase enzymatic activities present in periplasmic extracts of the Im7 folding biosensor were monitored in pst WT and pstSCA mutant strains using a spectrophotometric assay. Data are represented as mean ± SEM from three technical replicates (unpaired t-test,** P&lt;0.01, *** P&lt;0.001 comparison to MG1655; ns, not significant). "
NCBI gene IDs:
"Im7: 
pstS: 948237"