EMBO/SourceData huggingface dataset:
Figure legend: "NIX and BNIP3 are required for mitophagy induced by MLN4924 and other HIF1α stabilisers. Parental HeLa cells and NIX/BNIP3 double knockout (NIX/BNIP3 DKO) HeLa cells expressing mt-Keima were treated with DFP (1 mM), MLN4924 (0.5 μM), or DMOG (10 nM) for 24 hours and analysed by live-cell confocal microscopy. The emission signals obtained after excitation with the 458 nm laser (neutral pH) or 561 nm laser (acidic pH) are shown in green and magenta, respectively. Quantification of mitophagy shown in panel A. Mitophagy is represented as the ratio of mt-Keima 561 nm fluorescence intensity to mt-Keima 458 nm fluorescence intensity for individual cells normalised to the mean of the untreated condition. In B , the measurements from individual cells are represented as translucent grey dots, and the mean ratio from each independent experiment is represented by coloured circles. The centre lines and bars depict the mean of the averaged independent replicates +/- standard deviation. N=4. A minimum of 50 cells were analysed for each condition within each individual replicate experiment, and over 300 cells were analysed for each condition in total. P values were calculated based on the mean ratio values from independent experiments using one-way ANOVA (*P&lt;0.05, **P&lt;0.005, ***P&lt;0.001, ****P&lt;0.0001)."
NCBI gene IDs:
"mt-Keima: 
BNIP3: 664
NIX: 665"
Figure legend: "Analysis of NIX and BNIP3 protein levels after MLN4924, DMOG, or DFP treatments. HeLa cells or HeLa NIX/BNIP3 DKO cells were treated with MLN4924, DMOG, or DFP for 24 hours. Total-cell lysates corresponding to conditions in panel A were subject to immunoblotting."
NCBI gene IDs:
"BNIP3: 664
NIX: 665"
Figure legend: "Inhibition of HIF1α with echinomycin prevents DFP-induced mitophagy but not MLN4924-induced mitophagy. U2OS cells expressing mt-Keima were treated with the indicated drugs for 24 hours and analysed by live-cell confocal microscopy. Quantification of mitophagy shown in panel D. In F, the measurements from individual cells are represented as translucent grey dots, and the mean ratio from each independent experiment is represented by coloured circles. The centre lines and bars depict the mean of the averaged independent replicates +/- standard deviation. N=4. A minimum of 50 cells were analysed for each condition within each individual replicate experiment, and over 300 cells were analysed for each condition in total. P values were calculated based on the mean ratio values from independent experiments using one-way ANOVA (*P&lt;0.05, **P&lt;0.005, ***P&lt;0.001, ****P&lt;0.0001)."
NCBI gene IDs:
"mt-Keima: "
Figure legend: "Re-expression of FBXL4 into FBXL4-defective CRISPR lines reduces the levels of NIX and BNIP3 in FBLX4 deficient clones. FBXL4-deficient 2G10 and FBXL4-deficient 1D4 cell lines were stably transduced with a doxycycline-inducible FBXL4-HA construct. Cells were treated with doxycycline for the indicated times prior to immunoblotting with the specified antibodies."
NCBI gene IDs:
"CRISPR: 
HA: 
FBLX4: 26235
FBXL4: 26235"
Figure legend: "FBXL4 localises to the mitochondrial outer membrane. Cells transiently transfected with FBXL4-HA-C were treated with DFP for 24 h. Cells were stained with an anti-HA antibody (to recognise FBXL4) and either TOM20 (an outer mitochondrial membrane protein) or TIM50 (an inner mitochondrial membrane protein). The line scan intensity profiles for FBXL4 (green) and TOM20/TIM50 (magenta) represent fluorescence intensity (y-axis) plotted against distance (x-axis). Scale bars = 5 μm."
NCBI gene IDs:
"HA: 
FBXL4: 26235"
Figure legend: "FBXL4 is a proximity interactor of NIX and BNIP3. Cells expressing inducible BirA-BNIP3, BirA-NIX and BirA control were transduced with a lentiviral vector expressing FBXL4, as indicated. Cells were treated with doxycycline for 48 hours (to induce BirA-bait protein expression), biotin for 24 hours (for the biotinylation reaction), and, where indicated, MLN4924 for 24 hours (to stabilise NIX and BNIP3). Streptavidin-coupled beads were used to capture the biotinylated proteins. FBXL4 was specifically detected in the eluate from BirA-BNIP3 and BirA-NIX compared with BirA-alone."
NCBI gene IDs:
"BirA: 948469
BNIP3: 664
NIX: 665
FBXL4: 26235"
Figure legend: "NIX and BNIP3 polyubiquitylation depends on FBXL4. U2OS or U2OS-FBXL4 KO cells were co-transfected with TR-TUBE and either myc-BNIP3 or myc-NIX, as indicated. Cell lysates obtained 48 hours post-transfection were immunoprecipitated with anti-FLAG beads, and the immunoprecipitates were analysed by immunoblotting using anti-myc antibody (to detect ubiquitylated NIX or BNIP3). The line on the right marks a ladder of bands corresponding to polyubiquitylated myc-BNIP3 or myc-NIX"
NCBI gene IDs:
"myc: 
BNIP3: 664
NIX: 665
FBXL4: 26235"
Figure legend: "Loss of FBXL4 leads to an increase in mitophagy, which can be reduced by re-expression of FBXL4. U2OS mt-Keima FBXL4 KO clones (2G10 and 1D4) expressing doxycycline-inducible wild-type FBXL4-HA were treated with doxycycline for 72 hours. The emission signals obtained after excitation with the 458 nm laser (neutral pH) or 561 nm laser (acidic pH) are shown in green and magenta, respectively."
NCBI gene IDs:
"HA: 
mt-Keima: 
FBXL4: 26235"
Figure legend: "Depleting NIX/BNIP3 via siRNA reduces the increased mitophagy caused by FBXL4 deficiency. U2OS mt-Keima cells and U2OS mt-Keima FBXL4 KO 2G10 cells were transfected with siRNAs targeting both NIX and BNIP3 (NIX/BNIP3 si) or non-targeting siRNA (NT si). UT=untransfected."
NCBI gene IDs:
"mt-Keima: 
BNIP3: 664
NIX: 665
FBXL4: 26235"
Figure legend: "Corresponding cells from D-E were harvested for immunoblotting to analyse the extent of NIX and BNIP3 reduction after siRNA transfection."
NCBI gene IDs:
"BNIP3: 664
NIX: 665"
Figure legend: "Hyperstable NIX deletion mutants increase mitophagy compared with wild-type NIX. Hela Flp-in NIX KO mt-Keima cells stably expressing inducible NIX(WT), FLAG-NIX∆151-170, or FLAG-NIX∆171-184 were treated with doxycycline for 48 hours Mitophagy was visualised as in A and quantified as in B. Hyperstable BNIP3 deletion mutants increase mitophagy compared with wild-type NIX. Hela Flp-in NIX/BNIP3 DKO mt-Keima cells stably expressing BNIP3 deletion mutants were treated with doxycycline for 48 hours translucent grey dots represent measurements from individual cells. Coloured circles represent the mean ratio from independent experiments. The centre lines and bars represent the mean of the independent replicate means +/- standard deviation. N=3. At least 200 cells were analysed per condition. P values were calculated based on the mean values using a one-way ANOVA (*P&lt;0.05, **P&lt;0.005, ***P&lt;0.001, ****P&lt;0.0001). Scale bars = 20 μm."
NCBI gene IDs:
"FLAG: 
mt-Keima: 
BNIP3: 664
NIX: 665"
Figure legend: "FBXL4 patient-derived variants exhibit reduced efficiency compared to wild-type FBXL4 in mediating the downregulation and destabilization of NIX and BNIP3. U2OS FBXL4 KO (2G10) cells were rescued with constructs expressing wild-type FBXL4-HA, FBXL4(F-box mut), FBXL4(∆MTS), or specified patient variants. Cells were treated with cycloheximide for 3 hours prior to harvesting. Samples were lysed, and immunoblotting was performed. GFP serves as a marker of transduction efficiency/transgene expression. EV = empty vector."
NCBI gene IDs:
"HA: 
FBXL4: 26235"
Figure legend: "FBXL4-Arg482Trp and FBXL4-Gly568Ala patient variants are less efficient than FBXL4-wild-type at assembling into a complex with SKP1 and CUL1. FBXL4-KO cells expressing wild-type FBXL4-HA or FBXL4 variants were harvested and lysed. Whole-cell extracts were subjected to immunoprecipitation (IP) with anti-HA agarose beads and immunoblotting, as indicated."
NCBI gene IDs:
"HA: 
FBXL4: 26235"
Figure legend: "NIX/BNIP3 depletion mediated by siRNA reduces mitophagy in FBXL4-deficient patient fibroblast cells. FBXL4-deficient patient fibroblasts were transfected with siRNAs targeting both NIX and BNIP3 (NIX/BNIP3 si) or non-targeting siRNA (NT si) (left). Live-cell confocal microscopy was performed to visualise mitophagy and quantification was performed as in D (middle). The siRNA-mediated depletion of NIX and BNIP3 was evaluated by Western blotting (right). translucent grey dots represent measurements from individual cells. Coloured circles represent the mean ratio from independent experiments. The centre lines and bars represent the mean of the independent replicates +/- standard deviation. in H, N=3. Over 100 cells were analysed per condition. P values were calculated based on the mean values using a one-way ANOVA (*P&lt;0.05, **P&lt;0.005, ***P&lt;0.001, ****P&lt;0.0001). Scale bar = 20 μm.­ "
NCBI gene IDs:
"BNIP3: 664
NIX: 665
FBXL4: 26235"
Figure legend: "Ubiquitylation of endogenous NIX is restored upon expression of FBXL4 in patient-derived cells. Patient derived fibroblasts were transfected with FLAG-tagged TR-TUBE, as indicated. FLAG-tagged TUBE protein was affinity purified from the corresponding lysates using FLAG-beads, and the precipitates were analysed by immunoblotting using antibodies to endogenous NIX."
NCBI gene IDs:
"FLAG: 
FBXL4: 26235"
Figure legend: "(B) UbL domains mediated degradation of His3 fusion proteins with a Su9 tail, and affected yeast growth under selective condition (+3-AT, -his). Replacing UbL domains with DHFR domains rescued the growth defect. Cells expressing His3 fusion proteins in late log phase were serially diluted and stamped on selective plates. Plates were incubated at 30 ˚C for 3 daysfor imaging."
NCBI gene IDs:
"His3: 
DHFR: 854411
Su9: 948275"
Figure legend: "(B) Fluorescence profiles of cells expressing different substrates monitored by flow cytometry. UbLRad23 targeted YFP-Su9 protein for degradation. Removing the Su9 tail or replacing UbLRad23 with a DHFR domain stabilizes YFP protein in cells."
NCBI gene IDs:
"Su9: 948275"
Figure legend: "(B) In vitro degradation of Mdy2 proteins by purified S. cerevisiae proteasome. Mdy2 (red solid circles), Mdy2ΔN-95 (blue solid circles) and Mdy2-95 (green solid circles) were degraded. Removing the N-terminal domain of Mdy2 (Mdy2ΔN, black solid circles) or adding its binding partner Get4 (red open squares, dash line) or the proteasome-binding competitor UbLMdy2 (red open diamonds, dotted line) stabilized Mdy2Get4. When Mdy2 binding buried the N-terminal domain, addition of a 95 amino-acid tail derived from S. cerevisiae cytochrome b2 at the Cterminus restored degradation of Mdy2 (green open squares, dotted line). The graph plots the amount of substrate estimated by electronic autoradiography in SDS-PAGE gel bands over time as normalized to the initial substrate amount as described in Methods. Data points represent mean values determined from at least three repeat experiments; error bars indicate s.e.m."
NCBI gene IDs:
"cytochrome b2: 854950
Get4: 854335
Mdy2: 854038"
Figure legend: "(C) Degradation of Mdy2 in vivo. The steady-state accumulation of overexpressed C-terminally HA-tagged full-length Mdy2 and a mutant in which the N-terminal domain was deleted (Mdy2ΔN) in S. cerevisiae, in the absence of Get4, the presence of endogenously expressed Get4 or overexpressed Get4. Overexpressed Get4 was detected through an N-terminal Flag-tag."
NCBI gene IDs:
"Get4: 854335
Mdy2: 854038"
Figure legend: "(D) Steady-state accumulation of endogenous Mdy2 in S. cerevisiae, with deleted (get4∆) or endogenous (wt) Get4 in the presence or absence of the proteasome inhibitor bortezomib. Protein levels were determined by western blotting for the HA-tag, Flag-tag, Mdy2 or Scs2 of SDS-PAGE gels of S. cerevisiae protein extracts. Protein loading levels in each lane were estimated in all lysates by western blotting for the integral endoplasmic reticulum membrane protein Scs2."
NCBI gene IDs:
"get4: 854335
Get4: 854335"
Figure legend: "(B) In vitro degradation of Ubp6 proteins by purified S. cerevisiae proteasome. Full-length Ubp6 (Ubp6, black open circles) and a UbL deletion mutant (Ubp6ΔN, green open circles) escaped proteasome degradation. Attachment of a Cterminal 95 amino-acid tail derived from S. cerevisiae cytochrome b2 allowed the proteasome to degrade the Ubp6 proteins (Ubp6-95, black solid circles and Ubp6ΔN-95, green solid circles). The graph plots the amount of substrate estimated by electronic autoradiography in SDS-PAGE gel bands over time as normalized to the initial substrate amount as described in Methods. Data points represent mean values determined from at least three repeat experiments; error bars indicate s.e.m."
NCBI gene IDs:
"cytochrome b2: 854950
Ubp6: 850562"
Figure legend: "C) Degradation of Ubp6 proteinsin vivo. The steady-state accumulation of N-terminally HA-tagged Ubp6 and mutants in S. cerevisiae. Ubp6 and Ubp6ΔN are stable in yeast and not further stabilized by bortezomib. Attachment of a Su9 tail to the Cterminus lead to the degradation of Ubp6 and Ubp6ΔNproteins and the proteins were stabilized by bortezomib treatment."
NCBI gene IDs:
"Su9: 948275
Ubp6: 850562"
Figure legend: "(D) The steady-state accumulation of endogenous Ubp6 and Ubp6 derivatives with short (19 amino acid) or long (54 amino acid) tails at their C-termini. The 19 amino-acid tail destabilized Ubp6 and the 54-amino-acid tail lead to complete depletion of Ubp6. Protein levels were determined by western blotting for the HA-tag, Ubp6 or Scs2 in SDS-PAGE gels of S. cerevisiae protein extracts. The proteasome was inhibited by 100 µM bortezomib as indicated. Protein loading levels in each lane were normalized in all lysates by western blotting for the integral endoplasmic reticulum membrane protein Scs2."
NCBI gene IDs:
"Ubp6: 850562"
Figure legend: "(a) Electron micrographs of the IM (black arrowheads) associated with the ER (black arrows) from Atg4BC74A-expressing NIH 3T3 cells. The IM appears to expand between the two ER cisternae and forms an autophagosome. The white arrowhead indicates vesicular clusters visible in the vicinity of the IM. The white arrow indicates the autophagosome."
NCBI gene IDs:
"Atg4B: 66615"
Figure legend: "(c) Localization of GFP-DFCP1 with Atg16L1 in both control and Atg4BC74A-expressing cells. Insets show enlarged images of boxed regions."
NCBI gene IDs:
"Atg4B: 66615"
Figure legend: "(d-g) Gold-enhanced immunogold electron microscopy revealed that GFP-DFCP1 localized to ER-IM complexes in both control (d, e) and Atg4BC74A-expressing cells (f). Immunogold particles are indicated by small arrows. No specific labelling was observed on ER-IM complexes in cells expressing GFP (g). Large arrows and arrowheads indicate the ER and the IM, respectively. Scale bars, 200 nm in a and d-g, an d 10 μm in c."
NCBI gene IDs:
"Atg4B: 66615"
Figure legend: "Atg4BC74A-expressing cells (for Atg16L1) or control cells (for PDI, COP II and β-COP) were labelled with specific antibodies against the indicated markers. The left and right anti-Atg16L1 and anti-PDI images are from two different ER-IM complexes. Arrows, arrowheads and open arrowheads indicate the ER, IMs and VTCs, respectively. Small arrows in the image from the anti-COP II experiment indicate immunogold particles. Scale bars, 200 nm."
NCBI gene IDs:
"Atg4B: 66615"
Figure legend: "Representative  images for the TIF analysis shown in (C). Shown are  POT1Y223C/- hESCs and an unedited wild-type  hESC clone (WT/WT) isolated in parallel, as well as a POT1 WT/Q623H hESC and a contemporaneously isolated hECS clones (WT/WT). Scale bars = 10µm.   Box and whisker plot (showing min, max and average) quantifying telomere-dysfunction induced foci (TIF) in two  POT1 Y223C/-  hESC lines, one POT1  Q623H/Q623H, and one POT1WT/Q623H  hESC lines compared to wild-type unedited control hESCs. Cells were analyzed 3 months post targeting. Cells were stained for TRF1  and γ-H2Ax. POT1Y223C/- cells have increased TIFs compared to an unedited wild-type cell clone, isolated and cultured in parallel (P&lt;0.01, two-tailed Mann-Whitney test, one biological replicate).   "
NCBI gene IDs:
"POT1: 25913"
Figure legend: "B) TIF analysis of wild-type, POT1WT/Y223C hESCs, and the indicated controls using antibodies against TRF1 and γ-H2AX. Scale bars = 10µm. Lentiviral expression of TPP1∆PBD served as a positive control to demonstrate that cells have an intact telomere DNA damage  response. TPP1∆PBD infected cells were analyzed 72h post infection and without selection."
NCBI gene IDs:
"POT1: 25913
TPP1: 1200"
Figure legend: "C) Representative genotyping outcomes of hCD34+ cells nucleofected sgRNA-1, xenografted  and isolated from the bone marrow 16 weeks after transplantation into  mice  determined by NGS. Data information: The vertical dashed line shows the site of CAS9 cut and the base pairs noted in red show insertion. A dash inside a gray box indicates a deleted base pair."
NCBI gene IDs:
"CAS9: 57852564"
Figure legend: "Tumour cells were syngeneically and orthotopically co-grafted with mouse FAK-WT or FAK-KD fibroblasts. Thirty-eight days after injection, mice were euthanized. (A) Primary tumour mass quantification. Values are means ± SEM from at least 7 mice per group."
NCBI gene IDs:
"FAK: 14083"
Figure legend: "(D) Impact of conditioned media (CM) secreted by activated mouse fibroblasts expressing FAK-WT or FAK-KD on mouse BMDM-derived macrophage polarization. Relative frequencies of macrophages expressing CD80, CD86, Nos2, CD206, CMH2, Dectin, and CD163 markers acquired by flow cytometry and processed using FlowJo software."
NCBI gene IDs:
"FAK: 14083"
Figure legend: "(F) Quantitative real-time PCR conducted with gene-specific primers (CCL2, CCL5 and M-CSF) on four different FAK-I-treated (16h) and non-treated CAFs for gene expression. The normalized fold change ± SEM was calculated using the delta-delta Ct method with RSP16 as a control gene; values are mean +/- SEM. Each individual patient-derived activated fibroblasts was assigned to a specific symbol"
NCBI gene IDs:
"RSP16: 
CCL2: 6347
CCL5: 6352
M-CSF: 1435"
Figure legend: "(A) Representative images of three independent scratch wound assays of co-cultivated red-labelled pancreatic tumour cells with either FAK-WT (wild-type) or FAK-KD (Kinase-dead) green-labelled fibroblasts at time zero and 46 hours (corresponding to the wound closure of co-cultured FAK-WT fibroblasts with tumour cells)."
NCBI gene IDs:
"FAK: 14083"
Figure legend: "(I) 3D reconstitution of cell invasion into collagen matrix from spheroid composed of green-labelled FAK-WT or FAK-KD fibroblasts plus red-labelled pancreatic tumour cells (ratio 1/1). Shown are representative 3D reconstitution of spheroid invasion into 2mg/ml collagen I matrix obtained using Zen software. (J-K) Quantification of fibroblast (J) or tumour cell (K) invasion within collagen matrix. Values are means ± SEM of 60 tracked cells from three independent experiments."
NCBI gene IDs:
"FAK: 14083"
Figure legend: "(A) Representative images of CK19 and α-SMA immunohistochemistry and Sirius red staining in serial sections of primary mouse tumour (graft of FAK-WT or FAK-KD fibroblasts + tumours cells at 38 days after injection) at the invasive front. Scale bar, 100 µm. Bottom: schematic organization of the cells (tumour cells in brown, CAFs in yellow and non-transformed epithelial cells in beige)."
NCBI gene IDs:
"FAK: 14083"
Figure legend: "(B) Representative images of ECM deposition (stained using Alexa Fluor 488 succinimidyl ester (NHS)) generated by FAK-WT or -KD activated fibroblasts during migration."
NCBI gene IDs:
"FAK: 14083"
Figure legend: " (A) Confocal images of ALIX (red), Asl (white), α-tubulin (green) and Hoechst (blue) in wild type (w1118), Sas-6c02901, DSpdG02143, cnnHK21 and γ-Tub23CPI NBs (scale bars, 5 μm). The insets show higher magnification of the boxed centrosomes"
NCBI gene IDs:
"cnn: 36491
γ-Tub23C: 33501
Sas-6: 43555
DSpd: 39850"
Figure legend: "(B) The centrosomal fluorescence intensity of ALIX was measured and the graph shows the average intensity (relative to w1118 intensity) of three separate experiments (±S.E), where in total 78 w1118, 73 Sas-6c02901, 45 DSpdG02143, 61 cnnHK21 and 26 γ-Tub23CPI NBs were analyzed. Statistically significant differences are indicated (*p&lt;0.05 and **p&lt;0.01, Student's t-test)"
NCBI gene IDs:
"cnn: 36491
γ-Tub23C: 33501
Sas-6: 43555
DSpd: 39850"
Figure legend: "(D) HeLa cells were transfected with control, Cep192 or Cep215 siRNA. Efficient depletion of Cep192 and Cep215 was confirmed by Western blotting analysis, which also showed unchanged expression levels of ALIX. Unspecific bands, below Cep192 and above Cep215, are indicated with *"
NCBI gene IDs:
"Cep215: 55755
Cep192: 55125"
Figure legend: "*. (E) Control, Cep192 or Cep215 siRNA-transfected HeLa cells were immunostained with anti-ALIX (red), anti-α-tubulin (green) and Hoechst (blue). Confocal micrographs are presented and the insets show the indicated centrosomes (arrows) at higher magnification (scale bars, 5 μm)"
NCBI gene IDs:
"Cep215: 55755
Cep192: 55125"
Figure legend: "(F) The centrosomal ALIX fluorescence intensity was measured and the graph shows the average intensity (relative to control siRNA cells) of three independent experiments (±S.E), where in total 41 control siRNA, 40 Cep192 siRNA and 39 Cep215 siRNA-transfected cells were analyzed, **p&lt; 0.01, Student's t-test"
NCBI gene IDs:
"Cep215: 55755
Cep192: 55125"
Figure legend: " (A) Brains were dissected from w1118, alix1, alix-l/CyO; alix1 and mud4 larvae and stained with anti-Bazooka (red), anti-Cnn (white) and Hoechst (blue). Representative confocal images are shown. Scale bars, 5 μm. The graph summarizes the average variation of the relative spindle angles in w1118 (n=74), alix1 (n= 96), alix-l/CyO; alix1 (n= 78) and mud4 (n=32) NBs (four separate experiments) (±S.E). Compared to w1118 or alix-l/CyO; alix1 NBs both  alix1 and mud4 NBs showed greater variation in MS orientation (***p&lt;0.001, ANOVA) "
NCBI gene IDs:
"alix: 43330
mud: 44839"
Figure legend: "(C) Brain lysates of w1118, alix1 and alix-l/CyO; alix1 larvae were immunoblotted for ALIX, α-tubulin and GAPDH (loading control)"
NCBI gene IDs:
"alix: 43330"
Figure legend: "(D) Time-lapse imaging of Asl-YFP, α-tubulin-GFP in control (left panel) and alix3 (right panel) mutant SOPs of the pupal notum. SOPs were identified by the apical meshwork of MTs (not shown). Time is indicated in min:sec. Mitotic cells are indicated with arrows. Scale bar represents 5 μm"
NCBI gene IDs:
"alix: 43330"
Figure legend: "(E) Quantification of the rotation angle (±S.E) in control (n=26) and alix3 (n=45) mutant SOPs (at least three separate experiments). **p&lt;0.01 (Student's t-test) compared to control"
NCBI gene IDs:
"alix: 43330"
Figure legend: "(G) Western blotting analysis of lysates from control Asl-YFP, α-tubulin-GFP and Asl-YFP, α-tubulin-GFP; alix3 pupae showed absence of ALIX in the alix3 homozygous mutant and unchanged expression of α-tubulin and GAPDH (loading control)"
NCBI gene IDs:
"alix: 43330"
Figure legend: " (A) Ovaries of adult female flies (w1118, alix1 and alix-l/CyO; alix1) were stained with rhodamine-phalloidin (red), anti-α-tubulin (green), anti-Cnn (white) and Hoechst (blue). Typical confocal micrographs of metaphase FECs are presented and the apical membrane facing the egg chamber lumen is indicated with *. Scale bar, 5 μm. The MS orientation was measured and the graph shows the average percentage of FECs (w1118, n=45, alix1, n=45, alix-l/CyO; alix1, n=39, from three independent experiments) with the indicated relative spindle angle (±S.E). Statistically significant difference is indicated as ***p&lt;0.001 (ANOVA)"
NCBI gene IDs:
"alix: 43330"
Figure legend: "(C) Lysates of ovaries from w1118, alix1 and alix-l/CyO; alix1 flies were immunoblotted for ALIX, α-tubulin and GAPDH (loading control)"
NCBI gene IDs:
"alix: 43330"