EMBO/SourceData huggingface dataset:
Figure legend: "B qPCR  analysis of mRNA levels of  interleukin-1β (IL-1ß) (B1) and -6 (IL-6) (B2), cyclin-dependent kinase inhibitor 1 (p21) (B3) transforming growth factor-β  (TGF-β) (B4), and senescence markers (cell-cycle inhibitor p16, INK4a) (B5), Collagen Type I Alpha 1 Chain (Col1a1) (B6) in diaphragm of 20-month-old WT (n=6), mdx (n=5) and mdx/CD38-/- (n=5) mice. Data information: Each dot of the graphs represents a mouse. B in duplicate. After normality and variance comparison tests, significance was assessed using: B1-6: ANOVA followed by Fisher's LSD test. Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494
cyclin-dependent kinase inhibitor 1: 12575
p21: 12575
cell-cycle inhibitor p16: 12578
INK4a: 12578
Col1a1: 12842
Collagen Type I Alpha 1 Chain: 12842
IL-1ß: 16176
interleukin-1β: 16176
IL-6: 16193
TGF-β: 21803
transforming growth factor-β: 21803"
Figure legend: "A NAD+ levels in  limb muscles of 20-month-old WT (n=7), mdx  (n=5) and mdx/CD38-/- (n=6) mice. Data information: Each dot of the graphs represents a mouse. in duplicate; After normality and variance comparison tests, significance was assessed using: ANOVA followed by Fisher's LSD test; Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "B CD38 expression: western blot  analysis of CD38 (B1) protein in  limb of WT and mdx mice (n=6 per group). Vinculin  is used as housekeeping protein control and the dot plots show the ratio of  CD38/vinculin.  qPCR analysis of CD38 mRNA (B2) in  limb of 20-month-old WT and mdx mice  (n=4 per group). Data information: Each dot of the graphs represents a mouse. one value/mouse; After normality and variance comparison tests, significance was assessed using: B1: unpaired Welch's t-test; B2 : unpaired Student's t-test Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "C Histograms showing the  grip duration (latency to fall) (C1) and the limb force (C2) measured by a  grip test in WT (n=89), mdx (n=52, 53 respectively) and mdx/CD38-/- (n=58)  mice (age: 9 to 26 months old). Data information: C1 one value/mouse; C2 in triplicate. After normality and variance comparison tests, significance was assessed using: : unpaired Student's t-test; Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "D Images showing  muscle fiber  typology revealed by  immunostaining. Localization  of the slow MyHC (Type I) fiber  and fast  MyHCs (Type IIa  and IIb/X) fibers, along with  laminin (red) on transverse cross-sections from soleus and tibialis (TA) of WT, mdx  and mdx/CD38-/- mice. Scale bars: 100 µm. Histogram showing the percentage of I, IIa and IIb/X fiber type distribution in soleus  and TA of WT, mdx  and mdx/CD38-/- mice. Experiments performed in WT (n=3), mdx  (n=5) and mdx/CD38-/- (n=7)  mice for soleus; WT (n=5), mdx  (n=6) and mdx/CD38-/- (n=6) mice for TA. Data information: one value/mouse; After normality and variance comparison tests, significance was assessed using: D: Chi-square test; Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "E Fiber  size distribution in soleus from WT, mdx  and  mdx/CD38-/- mice. Data information: one value/mouse After normality and variance comparison tests, significance was assessed using: E: Kolmogorov-Smirnov test; Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "F Images displaying the collagen (blue) revealed by Masson's  trichrome staining in limb  of WT, mdx, and mdx/CD38-/- mice. The dot plot shows the quantification of collagen staining area (% total area) in limb of WT (n=4), mdx  (n=5) and mdx/CD38-/- (n=5)  mice. Scale bars: 200 µm. Data information: Each dot of the graphs represents a mouse. in triplicate. After normality and variance comparison tests, significance was assessed using: : ANOVA followed by Fisher's LSD test; Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "G Embryonic myosin expression  revealed by  immunostaining along with laminin  (green) on transverse cross-sections from limb of WT (n=5), mdx  (n=8) and  mdx/CD38-/- (n=8) mice. Scale bars: 50 µm. Dot plot showing the relative proportion of  embryonic myosin area in limb of WT, mdx  and mdx/CD38-/- mice. Data information: Each dot of the graphs represents a mouse. one value/mouse; After normality and variance comparison tests, significance was assessed using: G: Welch's ANOVA followed by Welch's t-tests. Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"CD38: 12494"
Figure legend: "B, New-born double knockout utrophin-dystrophin (mdx/utr-/-) mice and treatment with an  CD38  inhibitor. Mdx/utr-/- mice were subcutaneously injected with  K-rhein (0.6 and 2.5 mg/kg/d) for 4 weeks. B: Dot plots showing the treadmill performances of K-rhein-treated  mdx/utr-/- mice: distance traveled (B1), maximum speed reached (B2) and maximum running time (B3) (WT (NaCl) and mdx/utr-/-+ K-rhein 2.5 mg/kg/d n=10 mice per group; mdx/utr-/-(NaCl) and mdx/utr-/-+ K-rhein 0.6 mg/kg/d, n=12 mice per group). Data information: Each dot of the graphs represents a mouse. one value/mouse; After normality and variance comparison tests, significance was assessed using: ANOVA followed by Fisher's LSD test Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, #: p&lt;0.001 vs mdx/utr-/- ."
NCBI gene IDs:
"dystrophin: 13405
utr: 22288
utrophin: 22288"
Figure legend: "C New-born double knockout utrophin-dystrophin  (mdx/utr-/-) mice and treatment with an CD38 inhibitor. Mdx/utr-/- mice were subcutaneously injected with K-rhein  (0.6 and 2.5 mg/kg/d) for 4 weeks. C: Measurement of the grip duration (grid test) in WT (n=10), mdx/utr-/- (n=8) and K-rhein-treated mdx/utr-/- mice (n=10, 6 mice, respectively for the 0.6 mg/kg/d and 2.5 mg/kg/d doses). Data information: Each dot of the graphs represents a mouse. in triplicate. After normality and variance comparison tests, significance was assessed using: : Kruskal-Wallis followed by Dunn's test Values are expressed as means ± SEM. Significance: *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001, #: p&lt;0.001 vs mdx/utr-/- ."
NCBI gene IDs:
"dystrophin: 13405
utr: 22288
utrophin: 22288"
Figure legend: "A Mitochondrial translation products were labeled using a FUNCAT approach and [35S]Met labeling in control and TSFM patient fibroblasts. Representative epifluorescent microscopy and the autoradiography images are shown. Scale bars, 50 µm. Changes in mitochondrial protein synthesis were quantified for both approaches. For microscopy image analysis, &gt;300 cells from N = 3 independent experiments were analyzed for each cell type. Mann-Whitney U test, ****p &lt; 0.0001. For radiolabeling analyses, N =3 independent samples were quantified for each cell type. Student t test, **p &lt; 0.01. Error bars, +/- SEM. B As in A using control and COA6 KO HEK cells. Scale bars, 25 µm. For microscopy image analysis, ****p &lt; 0.0001, and for radiolabeling experiments, *p &lt; 0.05 were calculated. Error bars, +/- SEM."
NCBI gene IDs:
"COA6: 388753
TSFM: 10102"
Figure legend: "(b) Temperature-sensitive growth assay. Yeast strains (wild-type or deletion mutants, indicated on the left) were transformed with either an empty vector or a plasmid carrying an artificial guide RNA gene targeting U2 pseudouridylation at position 42 and 44 (indicated on the top), and grown at various temperatures (indicated at the bottom)."
NCBI gene IDs:
"U2: RF00004"
Figure legend: "(c) Blockage of pseudouridylation by deletion of specific pseudouridylase genes. RNAs isolated from wild-type and pseudouridylase-deletion strains (indicated on the top) were used for U2 pseudouridylation assay (CMC modification followed by primer-extension). Primer-extension pauses/stops correspond to ψ sites (indicated on the left and right). Note: The above-background signal of ψ42 in the snr81Δ lane (and to some extent, in the snr81Δ pus7Δ lane) is likely caused by the presence of strong ψ44 band. This is a primer-extension artifact, which often occurs when there is a strong ψ signal in the neighboring position. Alternatively, it is possible that having ψ at position 44 allows for an unidentified enzyme other than snR81 to modify U42 (albeit inefficiently) (Perhaps Pus1, which is responsible for ψ44 formation, can be a bit \"processive\"). Lanes 1-8 and lanes 9-16 are from two separate gels."
NCBI gene IDs:
"Pus1: 855889
pus7: 854417
snr81: 9164973
snR81: 9164973
U2: RF00004
U42: RF00004"
Figure legend: "(d) Restoration of U2 pseudouridylation by an artificial box H/ACA RNA. RNAs isolated from various yeast strains (indicated on the top), including the snr81Δ pusΔ strain that was transformed with an artificial guide RNA targeting positions 42 and 44 (lanes 9 and 10), were assayed for pseudouridylation [see legend to (c)]."
NCBI gene IDs:
"snr81: 9164973
U2: RF00004"
Figure legend: "(a) Growth assay using the ACT1-CUP1 reporter system. Yeast cells carrying the ACT1-CUP1 reporter were deleted of pseudouridylase genes (indicated on the left), and were then assayed for growth at 30°C on media containing various concentrations of [Cu2+] (indicated at the bottom)."
NCBI gene IDs:
"ACT1: 
CUP1: "
Figure legend: "(b) Growth rescue by restoration of pseudouridylation. The wild-type strain [from (a)] or the snr81Δ pusΔ strain [also from (a)], which exhibited the most severe growth-deficiency phenotype, was transformed with an empty vector or a plasmid carrying a pseudouridylation guide RNA gene targeting either position 42, position 44, or both (indicated). The resulting cells were assayed for growth at 30°C on media containing various concentrations of [Cu2+] (indicated at the bottom)."
NCBI gene IDs:
"snr81: 9164973"
Figure legend: "(d) Splicing assay using the wild-type and mutant ACT1-CUP1 reporters. RNAs isolated from the wild-type and the snr81Δ pusΔ strains (indicated on the top), which carry the wild-type ACT1-CUP1 reporter pre-mRNA or any of the mutant ACT1-CUP1 reporter pre-mRNAs [indicated, also see (c)], were used for splicing assay (primer-extension analysis). The un-spliced pre-mRNA, lariat intermediate, and spliced mRNA are indicated. A primer complementary to U6 was also used (as an internal control) in the assay, and the U6 band is indicated as well. In addition, the growth phenotype of each strain (in various concentrations of [Cu2+]) is also shown. Lanes 1-12, lanes 13 and 14, and lanes 15 and 16 are from separate gels."
NCBI gene IDs:
"ACT1: 
CUP1: 
snr81: 9164973
U6: RF00026"
Figure legend: "(e) Quantification of ACT1-CUP1 mRNA levels. Spliced ACT1-CUP1 mRNA levels were calculated relative to U6 in each lane. The quantification was based on three independent experiments."
NCBI gene IDs:
"ACT1: 
CUP1: 
U6: RF00026"
Figure legend: "(f) Relative improvement/reduction for the mutant reporters. Relative improvement or reduction in splicing was calculated by normalizing the splicing efficiency (mRNA/U6) of each mutant reporter to that of WT reporter (set at 1)."
NCBI gene IDs:
"U6: RF00026"
Figure legend: "Figure 3. Synthetic lethality assay. The Prp5 domain structure, and the mutations in some of the domains (indicated) are schematized at the top. Each of these PRP5 genes (wild-type or mutants, plasmid-borne) was used to replace the chromosomal PRP5. The numbers in parentheses are the relative ATPase activities of the wild-type (SAT) Prp5 and some of the Prp5 mutants tested before (Xu & Query, 2007). The U2 status, either containing Ψ42 and Ψ44 (WT-U2 and snR81Δ pus1Δ with pΨ42 or Ψ44) or lacking Ψ42 and Ψ44 (snR81Δ pus1Δ), is indicated (on the right). Upon transformation with the plasmids carrying PRP5 genes (wild-type or any of the mutants), cells were grown on the regular medium (-FOA) or on medium containing 5-FOA (+FOA)."
NCBI gene IDs:
"Prp5: 852539
PRP5: 852539
pus1: 855889
snR81: 9164973
U2: RF00004"
Figure legend: "(a) Prp5-U2 co-immunoprecipitation (IP). Wild-type (lanes 1 and 2) and snR81Δ pus1Δ (lane 3) strains, each containing a FLAG-tagged PRP5 gene (indicated on the top), were used for anti-FLAG IP in the absence (lane 1) or presence (lanes 2 and 3) of ATP. The precipitated Prp5 (WT Prp5-FLAG) as well as co-precipitated U1 and U2, detected by primer-extension, are indicated."
NCBI gene IDs:
"Prp5: 852539
PRP5: 852539
pus1: 855889
snR81: 9164973
U1: RF00003
U2: RF00004"
Figure legend: "(b) Quantification of co-precipitated U2 snRNA. For each lane [the WT lane and the snr81Δ pus1Δ lane, corresponding to lane 2 and lane 3 of (a), respectively], Prp5-co-precipitated U2 was normalized against co-precipitated U1 [(intensity of the U2 band) / (intensity of the U1 band)]. The snr81Δ pus1Δ lane was then further normalized against the WT lane [(the value of normalized U2 from the snr81Δ pus1Δ lane) / (the value of normalized U2 from the WT lane)]. Quantification was based on three independent experiments."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snr81: 9164973
U1: RF00003
U2: RF00004"
Figure legend: "(c) U2 expression level in wild-type and snR81Δ pus1Δ strains. Total RNA, isolated from the wild-type (lane 1) or snR81Δ pus1Δ (lane 2) strains, was used for primer-extension to measure the levels of U2 and U6. The U2 and U6 bands are indicated."
NCBI gene IDs:
"pus1: 855889
snR81: 9164973
U2: RF00004
U6: RF00026"
Figure legend: "d) Depletion of Prp5. Wild-type (lanes 1, 3 and 4) and snR81Δ pus1Δ (lanes 2 and 5) cells containing a FLAG-tagged PRP5 were lysed, and anti-FLAG IP was performed at a high concentration of salt. Cell extracts, before (lanes 1 and 2), and after [lanes 3 (mock), 4 and 5] anti-FLAG IP, were used for western analysis using anti-FLAG. The Prp5 band is indicated."
NCBI gene IDs:
"Prp5: 852539
PRP5: 852539
pus1: 855889
snR81: 9164973"
Figure legend: "(e) IP of reconstituted Prp5 (GAR)-U2. An equal amount of FLAG-tagged Prp5 (GAR) was added to the wild-type (lane 1) and snR81Δ pus1Δ (lane 2) cell extracts depleted of endogenous Prp5 [see (c)]. After the salt concentration was brought back to its original level, anti-FLAG IP was carried out. The Prp5 (GAR) band, detected by western, is indicated. The co-precipitated U1 and U2 bands, detected by primer-extension, are also indicated."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snR81: 9164973"
Figure legend: "f) Quantification of U2 that is co-precipitated with mutant Prp5 (GAR). For each lane [including the WT lane and the snr81Δ pus1Δ lane, corresponding to lane 1 and lane 2 of (e), respectively], U2 co-precipitated with Prp5 (GAR) was normalized against co-precipitated U1 [(intensity of the U2 band) / (intensity of the U1 band)]. The snr81Δ pus1Δ lane was then further normalized against the WT lane [(the value of normalized U2 from the snr81Δ pus1Δ lane) / (the value of normalized U2 from the WT lane)]. Quantification was based on three independent experiments."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snr81: 9164973
U1: RF00003
U2: RF00004"
Figure legend: "(g) Prp5-U2-pre-mRNA co-IP. Reconstitution was carried out as described in (d). Prior to IP, an equal amount of radiolabeled pre-mRNA was also added to each reaction. Anti-FLAG IP was then performed in the presence of ATP. The precipitated Prp5 (WT and GAR) band, detected by western, is indicated. The co-precipitated pre-mRNA, directly visualized after electrophoresis, is also indicated. The pre-mRNA input is also shown. In lanes 1 and 2, wild-type and snR81Δ pus1Δ cell extracts, depleted of Prp5, were reconstituted with the wild-type FLAG-Prp5. In lanes 3 and 4, wild-type and snR81Δ pus1Δ cell extracts, depleted of Prp5, were reconstituted with mutant FLAG-Prp5 (GAR)."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snR81: 9164973"
Figure legend: "(h) Quantification of pre-mRNA co-precipitated with Prp5 and U2. For each lane in (g), pre-mRNA co-precipitated with Prp5 (Wild-type or GAR) was normalized against the Prp5 (WT/GAR) band in the same lane (detected by Anti-FLAG) [(intensity of pre-mRNA) / (intensity of WT/GAR Prp5)]. Lanes 2,3,and 4 (see (g)) were then further normalized against lane 1 (see (g)) [(the value of normalized pre-mRNA from lane 2, 3 or 4 in (g)) / (the value of normalized pre-mRNA from lane 1 in (g))]. The numbers at the bottom of (h) correspond to the lane numbers of (g). Quantification was based on three independent experiments."
NCBI gene IDs:
"Prp5: 852539"
Figure legend: "(a) Prp5's ATPase activity assay using synthetic U2. The 5' sequence of S. cerevisiae U2 is shown (top). The three ψs (35, 42 and 44) as well as the branch site recognition sequence (underlined) are indicated. In the ATPase assay (bottom), no RNA (lane 3), or an equal amount of LiCl-precipitated RNA (lane 1) or tRNA (lane 2), a short U2 fragment (nts 1-76) (lane 4), a fully pseudouridylated short U2 fragment (nts 1-76) (lane 5), a long U2 fragment (nts 1-120) (lane 6), or a fully pseudouridylated long U2 fragment (nts 1-120) (lane 7), was used. The un-hydrolyzed ATP and hydrolyzed product ADP are indicated. Lanes 1 and 2, lanes 3-5, and lanes 6 and 7 are from separate gels."
NCBI gene IDs:
"Prp5: 852539
U2: RF00004"
Figure legend: "(b) Quantification of Prp5's ATPase activity activated by in vitro transcribed U2. Prp5's ATPase activity shown in (a) was calculated using the formula (ADP) / (ADT+ATP). The numbers at the bottom of (b) correspond to the lane numbers of (a). Quantification was based on three independent experiments."
NCBI gene IDs:
"Prp5: 852539
U2: RF00004"
Figure legend: "(c) U2-Prp5 binding assay. FLAG-Prp5 and a radiolabeled short (nts 1-76) (lanes 1 and 2) or long (nts 1-120) (lanes 5 and 6) U2 fragment, or FLAG-Prp5 and a radiolabeled fully pseudouridylated short (nts 1-76) (lanes 3 and 4) or long (nts 1-120) (lanes 7 and 8) U2 fragment, were incubated under the conditions used for ATPase activity assay. Anti-FLAG IP was then carried out. Co-precipitated (lanes 2, 4, 6 and 8) and un-precipitated (lanes 1, 3, 5 and 7) U2 RNAs were analyzed by electrophoresis. The U2 band is indicated."
NCBI gene IDs:
"Prp5: 852539
U2: RF00004"
Figure legend: "(d) Quantification of Prp5-U2 binding. Relative intensities of U2 bands shown in (c) were calculated [setting the un-precipitated uridine-containing U2 (lane 1 and 5) to 1]. The numbers at the bottom of (d) correspond to the lane numbers of (c). Quantification was based on three independent experiments."
NCBI gene IDs:
"Prp5: 852539
U2: RF00004"
Figure legend: "(e) Prp5's ATPase activity assay using cellular U2. U2 RNA isolated from the wild-type strain (lanes 2 and 4) or from the snR81Δ pus1Δ strain (lanes 6 and 8) was used for the ATPase activity assay. In odd-numbered lanes, no RNA was added. Both the wild-type Prp5 (lanes 1-4) and the mutant Prp5 (GAR) (lanes 5-8) were tested."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snR81: 9164973
U2: RF00004"
Figure legend: "(f) Quantification of Prp5's ATPase activity activated by U2 from cells. Prp5's ATPase activity shown in (e) was calculated using the formula (ADP) / (ADT+ATP). The numbers at the bottom of (f) correspond to the lane numbers of (e). Quantification was based on four independent experiments."
NCBI gene IDs:
"Prp5: 852539
U2: RF00004"
Figure legend: "(g) U2 level in the ATPase activity reactions. RNAs from lanes 2 and 4 in (c) were recovered, and U2 level was measured using primer-extension. Lanes 1-5, a titration of U2 snRNA. Lane 6, U2 from the wild-type strain [lane 2 of (c)]. Lane 7, U2 from the snR81Δ pus1Δ strain [lane 4 of (c)]."
NCBI gene IDs:
"pus1: 855889
snR81: 9164973
U2: RF00004"
Figure legend: "(a) Native gel analysis of pre-splicing complexes. Pre-splicing complex assembly was carried out in the test tube with Prp5-reconstituted cell extracts and labeled pre-mRNA. Lane 1, Prp5-depleted wild-type cell extract. Lane 2, Prp5-depleted wild-type cell extract, plus wild-type Prp5. Lane 3, Prp5-depleted snR81Δ pus1Δ cell extract, plus wild-type Prp5. Lane 4, Prp5-depleted wild-type cell extract, plus mutant Prp5 (GAR). Lane 5, Prp5-depleted snR81Δ pus1Δ cell extract, plus mutant Prp5 (GAR). The pre-splicing complexes A and B are indicated."
NCBI gene IDs:
"Prp5: 852539
pus1: 855889
snR81: 9164973"
Figure legend: "(b) DMS in vivo probing. After being exposed to DMS, yeast cells were lysed, total RNA collected, and primer-extension analysis carried out (left panel). Lane 1, cells that were not exposed to DMS. Lane 2, wild-type cells exposed to DMS. Lane 3, snR81Δ pus1Δ cells exposed to DMS. Lane 4, snR81Δ pus1Δ cells, transformed with a plasmid carrying an artificial guide RNA gene targeting positions 42 and 44, and exposed to DMS. Lane 5, snR81Δ pus1Δ cells, transformed with a plasmid carrying a control guide RNA gene with random guide sequences, and exposed to DMS. The nucleotides in parentheses are DMS-modified C and A residues that are one nucleotide after the actual primer-extension stops (indicated by the arrows). A partial U2 sequence (along with the BSL structure) is shown (right panel). DMS-modified adenosine and cytosine in the BSL are also indicated."
NCBI gene IDs:
"pus1: 855889
snR81: 9164973
U2: RF00004"
Figure legend: "Immunofluorescence microscopy detection of Tmem117  in the mouse hypothalamus  and pituitary: (A, B) Positive immunostaining  was observed in the PVN, the SON  and the posterior pituitary. (C, D) No immunostaining  was detected in the hypothalamus  and pituitary  of mice  with constitutive inactivation of Tmem117  in AVP neurons. Data information: 3V: 3rd ventricle, AP: Anterior pituitary, MZ:  Medial zone, opt: optic tract, PP: Posterior pituitary, PVN: Paraventricular nucleus, SCh: Suprachiasmatic nucleus, SON: Supraoptic nucleus. Scale bar=100μm for panels"
NCBI gene IDs:
"Tmem117: 320709"
Figure legend: "(H) Tmem117  mRNA levels in the PVN  [n=4 mice  per group]. (I)Tmem117  mRNA levels in the SON  [n=4 mice  per group Data information: INS: insulin, PVN: Paraventricular nucleus, SAL: saline, SON: Supraoptic nucleus. Lines correspond to the mean value per group and error bars represent ± SEM. Unpaired t-test; ns: p&gt;0.05, *p&lt;0.05, **p&lt;0.01."
NCBI gene IDs:
"Tmem117: 320709"
Figure legend: "(B)  C-Fos expression  in the SON  Data information: AVP: Vasopressin INS: insulin opt: optic tract, SAL: saline, Scale bar=100μm."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998"
Figure legend: "(G) Membrane potential  was increased comparably in response to 0.1 mM glucose  in both groups [n=7-9 cells per group]. (H) No change in  membrane potential in NR neurons  of both genotypes [n=9 cells per group]. (I) Membrane resistance  was decreased in 6 out of 9 AVPTM117WT and 6 out of 7 AVPTM117KO cells in response to 0.1 mM glucose  [n=7-9 cells per group]. (J) Membrane resistance in NR neurons  was stable for both genotypes [n=9 cells per group]. Data information: AVP: Vasopressin, d: day, GI: glucose  inhibited, NR: non-responding, Lines correspond to the mean value per group and error bars represent ± SEM. G-J: paired t-test for all graphs except Δ membrane potential (unpaired); nsp&gt;0.05, *p&lt;0.05, ***p&gt;0.001."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998
TM117: 320709"
Figure legend: "(A) Quantification of BiP  mRNA per cell in AVP-positive cells of the SON  [n=483-704 cells per group; each group consisted of 4 mice]. (B) Quantification of BiP  mRNA per cell in AVP-negative cells of the SON  [n=347-584 cells per group; each group consisted of 4 mice]. Data information: AVP: Vasopressin  For the violin plots dashed lines correspond to the median value and dotted lines to the quartile values. unpaired t-test ; *p&lt;0.05, ***p&gt;0.001."
NCBI gene IDs:
"BiP: 14828"
Figure legend: "(C) Fluorescence microscopy  detection of AVP  mRNA (green) and BiP  mRNA (magenta) in the SON. Data information: AVP: Vasopressin opt: optic tract  Scale bar=20μm."
NCBI gene IDs:
"AVP: 11998
Vasopressin: 11998
BiP: 14828"