EMBO/SourceData huggingface dataset:
Figure legend: "D) Representative confocal images of HeLa and TRIM16KO cells treated with (D) H2O2 (200 µM, 2h) and the samples were processed for IF analysis with Ub and p62 antibody. E) Representative confocal images of control siRNA and TRIM16 siRNA transfected cells treated with (E) H2O2, (200 µM, 2h), where IF analysis was conducted with Ub and p62 antibodies. F) The graph shows the the percentage of cells with Ub-p62 co-localized dots. Data from ≥10 fields (40X), n=3, Mean ±SD, **p &lt; 0.0003, *p &lt; 0.002 (Student's unpaired t-test). Data information: Unless otherwise stated, scale bar: 10 µm."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(G) Western blot (WB) analysis of detergent-soluble and -insoluble fractions of HeLa and TRIM16KO cells treated with MG132 (50 µM, 1h) and probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(J) Representative confocal images of HeLa and TRIM16KO cells treated with MG132 (20 µM, 2h) and processed for IF analysis with Ub and LC3B antibody."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(K, L) WB analysis of LC3B levels in lysates of HeLa and TRIM16KO cells treated with (K) Bafilomycin A1 (300 nM, 3h) alone or (L) with MG132 (10 µM, 4h) as indicated. L.E, Low exposure; H.E, high exposure."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(M) Representative confocal images HeLa and TRIM16KO cells treated with H2O2 (200 µM, 2h) and processed for IF analysis with Proteostat dye, Ub, and p62 antibody. To show the cellular details, the brightness of TRIM16KO cells images was increased more than control cells"
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(A) Western blot analysis of HeLa and TRIM16KO cell lysates probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(B) Western blot analysis of lysates from HeLa and TRIM16KO cells treated with or without MG132 and probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(C) Left panel, western blotting with HeLa and TRIM16KO cell lysates probed with indicated antibodies. Right panel, densitometric analysis (mean ±SD) of protein band intensity relative to actin, n=3, ***p &lt; 0.0005 (Student's unpaired t-test)."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "D-G (D, F) HEK293T cells were either transiently transfected with an empty vector or a Flag-tagged-TRIM16 expressing vector and immunoblotted with Flag or indicated antibodies. L.E, Low exposure; H.E, High Exposure. (E, G) Densitometric analysis (mean ±SD) of protein band intensity relative to actin, n=3, *p &lt; 0.05 (Student's unpaired t-test)."
NCBI gene IDs:
"Flag: 
TRIM16: "
Figure legend: "(H) WB analysis of HeLa and TRIM16KO lysates of cells treated with cycloheximide (100 µM) for the indicated period and probed with different antibodies as indicated. The blots of control and TRIM16KO cells are exposed for the equal duration and developed together on the same X-ray film. (I) Quantification of NRF2, p62, and KEAP1 band intensities relative to actin. n=3, **p &lt; 0.005, *p &lt; 0.05 (Student's unpaired t-test)."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(A, B) Co-IP analysis of the interaction between NRF2 and KEAP1 in absence and presence of TRIM16."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(D) Co-IP analysis of the interaction between KEAP1 and p62 in absence and presence of TRIM16."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(A-C) Analysis of NRF2 ubiquitination in absence and presence of TRIM16 by Co-IP assays using transiently transfected plasmid constructs as indicated. Two different variants of ubiquitin protein are used, one that can only be ubiquitinated at Lysine 48 residue (HA-K48-UB) and other that can be only ubiquitinated at Lysine 63 residue (HA-K63-UB). All other lysine residues are mutated. L.E, Low exposure; H.E, High Exposure."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "Analysis of NRF2 ubiquitination in absence and presence of TRIM16 by Ni-NTA pull-down assays using transiently transfected plasmid constructs as indicated. His-tagged ubiquitin which is mutated at all lysines except 63 position is used in these assays."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(F) Analysis of NRF2 ubiquitination in absence and presence of TRIM16 deletion variants by Co-IP assays using transiently transfected plasmid constructs as indicated."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(G) Western blot analysis of lysates from HeLa, TRIM16KO cells and TRIM16KO cells complemented with TRIM16 deletion constructs where cells were treated with MG132 (20 µM, 2h) and the blot is probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(H) Western blot analysis of lysates from HeLa, TRIM16KO cells and TRIM16KO cells complemented with TRIM16 deletion constructs where cells were treated with MG132 (20 µM, 2h) and the blot is probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(A) Western blot analysis of HeLa and TRIM16KO lysates of cells treated with increasing concentrations of H2O2 as specified and probed with antibodies as indicated."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(B) WB analysis of HeLa and TRIM16KO lysates of cells treated with 400 µM of H2O2 for different durations as indicated and probed with antibodies as shown. The red arrows indicate the time point where the cells were retreated with 400 µM of H2O2. L.E, Low exposure; H.E, High Exposure."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(D) WB analysis of HeLa and TRIM16KO lysates of cells treated with 2.5 µM (2 h) of As2O3."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(E-G) RNA isolated from HeLa and TRIM16KO cells, untreated or treated with MG132 (20 µM, 2h) were subjected to qRT-PCR with primers of genes as indicated. The fold induction in MG132 treated samples is calculated relative to untreated samples. Mean ±SD, n=3, *p &lt; 0.05, **p &lt; 0.005 (Student's unpaired t-test)."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "RNA isolated from control and NRF2 knockdown HeLa cells transfected with control vector or Myc-TRIM16 subjected to qRT-PCR with primers of genes as indicated. Mean ±SD, n=3, **p &lt; 0.005, ***p &lt; 0.0005 (Student's unpaired t-test)."
NCBI gene IDs:
"Myc: 
NRF2: 4780
TRIM16: 10626"
Figure legend: "(K) Western blot analysis of HeLa and TRIM16KO lysates of cells treated with h increasing concentrations of H2O2 as specified and probed with antibodies as indicated."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(M) Representative confocal images of control, Ubb and Ube2n siRNA transfected cells treated with H2O2 (200 µM, 2h) and IF was performed with Ub and p62 antibodies. Scale bar: 10 µm."
NCBI gene IDs:
"Ubb: 7314
Ube2n: 7334"
Figure legend: "(O) Western blot analysis of Ubb and Ube2n siRNA transfected cell lysates with antibodies as indicated."
NCBI gene IDs:
"Ubb: 7314
Ube2n: 7334"
Figure legend: "(A) Control or NRF2 siRNA transfected HeLa cells lysates were subjected to western blotting with indicated antibodies."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "(B, C) RNA isolated from untreated or MG132-treated (20 µM, 2h) control or NRF2 siRNA transfected cells were subjected to qRT-PCR with primers of genes as indicated. The fold induction in MG132 treated samples is calculated relative to untreated samples. Mean ±SD, n=3, *p &lt; 0.05 (Student's unpaired t-test). Data information: Unless otherwise stated, scale bar: 10 µm."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "WB analysis of control siRNA or NRF2 siRNA transfected lysates of HeLa cells, untreated or treated with MG132 (20 µM, 2h) as indicated and probed with antibodies as shown. L.E, Low exposure; H.E, High Exposure."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "WB analysis of control siRNA or NRF2 siRNA transfected lysates of HeLa cells, untreated or treated with H2O2 (200 µM, 2h) as indicated and probed with antibodies as shown. L.E, Low exposure; H.E, High Exposure."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "Left panels: Representative confocal images of control siRNA and NRF2 siRNA transfected cells treated with (F) MG132 (20 µM, 2h), where IF was conducted with Ub and p62 antibodies. Right panels: fluorescence intensity line tracing corresponding to a white line in zoom panel."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "Left panels: Representative confocal images of control siRNA and NRF2 siRNA transfected cells treated with (G) H2O2 (200 µM, 2h), where IF was conducted with Ub and p62 antibodies. Right panels: fluorescence intensity line tracing corresponding to a white line in zoom panel."
NCBI gene IDs:
"NRF2: 4780"
Figure legend: "(H) Hela cells, TRIM16KO cells, and TRIM16KO cells complemented with Myc-NRF2 or GFP-NRF2 were treated with MG132 (20 µM, 2h) and subjected to WB analysis with Myc, GFP and indicated antibodies."
NCBI gene IDs:
"GFP: 
Myc: 
NRF2: 4780
TRIM16: 10626"
Figure legend: "(I) Representative confocal images of Hela cells, TRIM16KO cells, and TRIM16KO cells complemented with Myc-NRF2 which were treated with MG132 (20 µM, 2h) and subjected to IF with Ub and p62 antibodies."
NCBI gene IDs:
"Myc: 
NRF2: 4780
TRIM16: 10626"
Figure legend: "WB analysis of detergent-soluble and -insoluble fractions of HeLa and TRIM16KO cells expressing either (A) Flag-p62 probed with Flag or indicated antibodies. L.E, Low exposure; H.E, High Exposure. Densitometric analysis of WB's normalized with α-Tubulin or Lamin-B1."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "WB analysis of detergent-soluble and -insoluble fractions of HeLa and TRIM16KO cells expressing either (C) GFP-polyQ74 probed with or GFP or indicated antibodies. L.E, Low exposure; H.E, High Exposure. Densitometric analysis of WB's normalized with α-Tubulin or Lamin-B1."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(H) Co-IP analysis of the interaction between two differently tagged TRIM16 variants."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(I) WB analysis of detergent-soluble and -insoluble DSS-cross-linked fractions of HeLa and TRIM16KO cells, untreated or treated with MG132 (10 µM, 4h) and probed with indicated antibodies."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(J) Co-IP analysis of the interaction between Flag-tagged TRIM16 and Myc-tagged TRIM16 full length and its domain deletion proteins."
NCBI gene IDs:
"Flag: 
Myc: 
TRIM16: 10626"
Figure legend: "(A) MTT assays performed at different time points with HeLa and TRIM16KO cells, untreated or treated with H2O2 (400 µM, 2h). Data, mean ±SD, n=3, *p &lt; 0.05 (Student's unpaired t-test)."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(B) Images of HeLa and TRIM16KO cells, untreated or treated with As2O3 (2.5 µM, 4h). Scale bar: 400 µm."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "Flow cytometry analysis of HeLa and TRIM16KO cells stained with Annexin-V/Propidium Iodide (double staining), untreated or treated with (C) As2O3 (2.5 µM, 4h)"
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "Flow cytometry analysis of HeLa and TRIM16KO cells stained with Annexin-V/Propidium Iodide (double staining), untreated or treated with (D) H2O2 (400 µM, 2h)"
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "Flow cytometry analysis of HeLa and TRIM16KO cells stained with Annexin-V/Propidium Iodide (double staining), untreated or treated with (E) puromycin (5 µg/ml, 6h)"
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "Flow cytometry analysis of HeLa and TRIM16KO cells stained with Annexin-V/Propidium Iodide (double staining), untreated or treated with (F) MG132 (20 µM, 8h)."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(G) Immunoblot blot analysis of HeLa and TRIM16KO lysates of cells treated with 5 µM of As2O3 for different durations as indicated and probed with antibodies as shown. L.E, Low exposure; H.E, High Exposure. Arrowheads indicate the cleaved form of PARP-1 and Casapse-3."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(A, B) Clonogenic assays were performed with HeLa and TRIM16KO cells, untreated or treated with As2O3 or MG132."
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(C) Tumor volumes of HeLa and TRIM16KO tumors at indicated time points. As2O3 is injected when tumor volume of all groups was >100mm3 (day 0 in the graph). Tumor volumes were measured on an interval as indicated. Mean ±SE, n=6 (each group), *p &lt; 0.05, **p &lt; 0.005, ***p &lt; 0.0005 (ANOVA). (D) Graph shows the % tumor regression (Tumor volume on day 1 of As2O3 treatment / Tumor volume on day of sacrifice X 100). Mean ±SE, n=6 (each group), **p &lt; 0.005 (Student's unpaired t-test). "
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(E) Representative images of HeLa and TRIM16KO tumors formed in the nude mice in the absence and presence of As2O3. (F) Representative pictures of dissected tumors. "
NCBI gene IDs:
"TRIM16: 10626"
Figure legend: "(B)  flg22-induced ROS production in Col-0 and fer-4 mutant roots  under  Pi-starvation conditions. Seedlings grown in either HP or LP media were treated with 1 μM  flg22, RALF23 or both  flg22 and RALF23 for 15 mins. Roots were harvested and stained with H2DCFDA. One representative root was shown. (C) Average H2DCFDA signal intensity in roots shown in (B). The fluorescence intensity was quantified with ImageJ. The data shown indicate the means ± SDs (n = 30, n refers to the number of  roots per group); n.s., not significant; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results. "
NCBI gene IDs:
"fer-4: 818622"
Figure legend: "(F)  RALF23 inhibits flg22-induced MAMP-responsive  FRK1 gene expression in a Pi  content-dependent manner. Seedlings  grown in either  HP or LP  media were treated with water or  flg22 for 15 mins and roots were harvested. The relative  expression of FRK1  was quantified via  RT-qPCR. The data shown indicate the means ± SDs (n = 3, n refers to technical replicates); n.s., not significant; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results. "
NCBI gene IDs:
"FRK1: 816436
RALF23: 820907"
Figure legend: "(A) The PSR induces the cleavage of  PRORALF23-GFP into mature  RALF23-GFP. The 35S promoter-driven RALF23-GFP line was grown in either HP  or LP media for 5 days and roots were harvested. Premature and mature  RALF23 levels were determined with anti-GFP. β-actin  was used as a loading control. Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"GFP: 
RALF23: 820907"
Figure legend: "(E) The PSR activates RALF expression  in a PHR1-dependent manner. Col-0  and phr1 seedlings were grown in LP media for 5 days and roots  were harvested. The relative  expression of RALFs was quantified via  RT-qPCR. The data shown indicate the means ± SDs (n = 3, n refers to technical replicates); n.s., not significant; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"PHR1: 828979
phr1: 828979"
Figure legend: "(G) PHR1  directly binds to RALF promoters, as shown via ChIP-PCR. The  Myc-labelled PHR1  overexpression line was immunoprecipitated  with anti-Myc. ChIP  analysis was performed on Col-0, CRY1-Myc and PHR1-Myc transformed plants, with Col-0 and CRY1-Myc transformed plants as negative controls. The data shown indicate the means ± SDs (n = 3, n refers to technical replicates); n.s., not significant; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"Myc: 
CRY1: 826470
PHR1: 828979"
Figure legend: "(B) PHR1 negatively regulates FLS2-BAK1 complex formation under  Pi-starvation conditions. Col-0,  phr1 mutant seedlings  were grown in either  HP or LP  media for 5 days, then lysing roots into  protoplasts, transformed with water or  PHR1-Myc plasmid and treated with flg22 for 6 hours. The amount of immunoprecipitated FLS2  and coimmunoprecipitated BAK1 were determined using anti-FLS2 and anti-BAK1 antibodies, respectively. Preimmune serum (IgG) was used as negative control. Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"Myc: 
phr1: 828979
PHR1: 828979"
Figure legend: "(D) Application of  RALF23/34 disrupted FLS2-BAK1 complex formation in phr1 mutants. phr1 mutants grown in  LP media were treated with water, 1 μM flg22, 1 μM RALF23, 1 μM RALF34 or both flg22  and RALF for 6 hours. The amount of immunoprecipitated FLS2 and  coimmunoprecipitated BAK1 were determined using anti-FLS2 and anti-BAK1 antibodies, respectively. IgG was used as a negative control. Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"phr1: 828979"
Figure legend: "(E)  RALF23/34 promoted the  growth  of  Pto DC3000 in phr1  mutants. Col-0 and phr1 mutant seedlings grown in LP  media were  root  inoculated (by soaking) with a bacterial suspension (OD600 = 0.2) for 2 min, and the number of bacteria from individual root was quantified at 5 dpi. The data shown indicate the means ± SDs (n = 18, n refers to the number of  roots  per group). The different lowercase letters indicate statistical significance (one-way ANOVA). Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"phr1: 828979"
Figure legend: "(F) FER negatively regulates FLS2-BAK1  complex formation under Pi-starvation conditions. Col-0, fer-4  mutant and FER/fer-4  complementation lines seedlings  were grown in either HP or LP  media for 5 days and treated with  flg22 for 6 hours. The amount of  immunoprecipitated FLS2  and coimmunoprecipitated BAK1 was determined using anti-FLS2 and anti-BAK1 antibodies, respectively. Preimmune serum (IgG) was used as a negative control. Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"FER: 824318
fer-4: 818622"
Figure legend: "(C) Pto DC3000  and B. subtilis upregulate  phosphate  absorption gene expression. Col-0, phr1  and  fer-4  mutant roots  were harvested 48 hours after inoculation with Pto DC3000 or B. subtilis  in Pi-sufficient or Pi-deficient condition, and the relative expression  of Pi-absorbing genes was quantified via RT-qPCR. The data shown indicate the means ± SDs (n = 3, n refers to technical replicates); *, p &lt; 0.05; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"fer-4: 818622
phr1: 828979"
Figure legend: "The fer-4 rhizosphere microbiome promotes plant growth and alleviates LP stress. Arabidopsis seeds were germinated on 1/2-strength MS media for 3 days and then transplanted to either HP or LP media for another 5 days. The seedlings were then transferred to vermiculite and inoculated with Col-0 or fer-4 rhizosphere microbiomes. (B), Images were taken 4 weeks after inoculation. Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"fer-4: 818622"
Figure legend: "(D) The  fer-4 rhizosphere microbiome  upregulated phosphate absorption  gene expression. The roots  of Col-0 were harvested after 4 weeks of inoculation with the Col-0 or  fer-4 rhizosphere microbiome  under LP  condition, and the relative  expression  of  Pi-absorbing genes was quantified via RT-qPCR. The data shown indicate the means ± SDs (n = 3, n refers to technical replicates); n.s., not significant; *, p &lt; 0.05; **, p &lt; 0.01 (Student's t test). Data information: All experiments were repeated three times with similar results."
NCBI gene IDs:
"fer-4: 818622"
Figure legend: "A FBN2 mRNA levels in multiple human tissues. Human tissue cDNAs were used as templates for PCR of FBN2, followed by agarose gel analyses. GAPDH levels served as loading controls (biological replicates, n=2)."
NCBI gene IDs:
"FBN2: 2201
GAPDH: 2597"
Figure legend: "B FBN2 mRNA levels in mouse tissues based on quantitative RT-PCR. Data were normalized based on levels in adipose tissues."
NCBI gene IDs:
"FBN2: 14119"
Figure legend: "C Comparison of FBN2 transcript levels between human and mouse placentas. Common primers for FBN2 and GAPDH in both species were used for RT-PCR analyses (biological replicates, n=3) ."
NCBI gene IDs:
"GAPDH: 
FBN2: 2201
FBN2: 14119"
Figure legend: "F For prokaryotic cell expression, a cDNA fragment corresponding to residues 2779 to 2912 of the human FBN2 coding region was appended with GST and 6-histidine tags and sub-cloned into the PGEX-6P-1 vector for expression in E. coli. Left panel: Coomassie blue staining of proteins in the SDS-PAGE gel before and after digestion with the PreScission protease. 1: Flow through; 2: Washes; 3: Before PreScission treatment; 4: After PreScission treatment. Right panel: Immunoblotting using placensin antibodies."
NCBI gene IDs:
"FBN2: 2201"
Figure legend: "B Placensin stimulation of glucose secretion (at 5h) and key gluconeogenesis gene transcripts (at 1h) in both cell types (biological replicates, n=4). Transcript levels for PEPCK and G6Pase were determined using quantitative RT-PCR. Treatment with glucagon (10 nM) served as positive controls."
NCBI gene IDs:
"G6Pase: 14378///14377///68401
PEPCK: 107690"
Figure legend: "B Knockdown of FBN2 in HTR-8/SVneo cells led to decreases in FBN2 transcripts and placensin secretion. Cells were treated with FBN2 siRNA (SiFBN2; 20 nM) or control siRNA (SiC; 20 nM) for 6h. Following media change, cells and conditioned media were collected at 48h after incubation. Placensin transcripts and proteins were determined by RT-PCR and ELISA (biological replicates, n=8), respectively. Lower panel: immunoblotting of secreted placensin by HTR-8/Svneo."
NCBI gene IDs:
"FBN2: 2201"
Figure legend: "D Placensin stimulation of transcript levels for MMP9 but not MMP2(biological replicates, n=12). Cells were treated with placensin for 24h before RT-PCR analyses of transcript levels."
NCBI gene IDs:
"MMP2: 4313
MMP9: 4318"
Figure legend: "B, C Colony-forming assays in H1299 (B) and A549 (C) lung cancer cells after knockdown by shRNA lentiviral constructs designed against DDX3 or vector control. Corresponding immunoblots displaying knockdown levels of DDX3. Mean from 3 replicates with SD."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "D Proliferation of A549 and H1299 cells after knockdown of DDX3. Mean from 3 replicates with SD. (A549 P = 0.011, H1299 P = 0.014; exponential curve fit, extra sum of squares F-test)."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "E β-galactosidase staining in parental A549 cells and A549 DDX3 knockdown cells displaying senescent cells identified by the blue color."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "K Survival analysis of lung cancer patients in low and high DDX3 expressing tumors (Kaplan-Meier curve and log-rank test, P = 0.016)."
NCBI gene IDs:
"DDX3: 1654"
Figure legend: "Confirmation of high expression of Twist1 and DDX3 in the lung tumors of the transgenic Twist1/KrasG12D mouse. Scale bar is 100 μm."
NCBI gene IDs:
"Kras: 16653
Twist1: 22160"
Figure legend: "Micro-CT images of transgenic Twist1/KrasG12D mice treated as in (C), before treatment and 1 week after treatment. Tumors are indicated by arrows and confirmed by H&amp;amp;amp;E staining of lung sections (lower panel). Scale bar is 250 μm.Quantification of tumor volume measured by micro-CT in Twist1/KrasG12D mice, as shown in (D). Significance was assessed by two-sided, unpaired t-test. Error bars represent SD."
NCBI gene IDs:
"Kras: 16653
Twist1: 22160"
Figure legend: "Micro-CT images of transgenic Twist1/KrasG12D mice treated as in (H), before treatment and 1 week after treatment.Quantification of tumor volume measured by micro-CT in Twist1/KrasG12D mice, as shown in (I) and expressed as relative tumor size. Significance was assessed by two-sided, unpaired t-test. Error bars represent SEM."
NCBI gene IDs:
"Kras: 16653
Twist1: 22160"
Figure legend: "A Cell cycle analysis of H1299 cells treated with shDDX3 and processed by flow cytometry. Knockdown of DDX3 led to a decrease of cells in S-phase and an increase of cells in G1-phase, indicative of a G1 arrest. Significance was assessed by two-sided, unpaired t-test. Error bars represent SD."
NCBI gene IDs:
"DDX3: 1654"