EMBO/SourceData huggingface dataset:
Figure legend: "Survival  of recipient mice  harboring SETD1A  mutant-expressing  leukemia  cells  after transplantation  (TX) was plotted (n = 5 / group). Data information: **P ≤ 0.01, *P ≤ 0.05  Kaplan-Meier for (F))."
NCBI gene IDs:
"SETD1A: 9739"
Figure legend: "SETD1A FLOS domain-binding proteins were identified by Co-IP MS. Full-length SETD1A and FO3 fragment were used as baits and overlapped proteins (red dots) were identified as SETD1A FLOS domain-binding proteins."
NCBI gene IDs:
"SETD1A: 9739"
Figure legend: "293T  cells were transfected with full-length SETD1A   alanine mutants and protein extracts were used for the Co-IP experiments."
NCBI gene IDs:
"SETD1A: 9739"
Figure legend: "F. MOLM-13 leukemia  cells were examined by  ChIP-seq  analyses against HA-tagged SETD1A, BuGZ, BUB3, H3K4me3 and H3K4me1. Heatmaps of SETD1A(+)/BuGZ(+) regions (upper) and SETD1A(-)/BuGZ(+) regions (lower) are shown."
NCBI gene IDs:
"SETD1A: 9739
BuGZ: 7756"
Figure legend: "Relative expression  levels of Bugz  were analyzed in Bugz  shRNA-expressing mouse MLL-AF9 leukemia  cells at day 3 post-treatment, respectively. Data from 3 biological replicates are shown. Data information: data are presented as mean ± SD. **P ≤ 0.01 (Student's t-test"
NCBI gene IDs:
"MLL: 214162
AF9: 70122
Bugz: 22680"
Figure legend: "Images are shown of representative karyotypes  in control, Bugz  and Bub3  shRNA-expressing leukemia  cells. Asterisks in images indicate the trisomy 15 in all cells analyzed. Red arrows indicate chromosomal aneuploidies. Representative images of sister-chromatid  separation or aneuploidy  in Bub3  shRNAs are shown in lower panels."
NCBI gene IDs:
"Bub3: 12237
Bugz: 22680"
Figure legend: "293T  cells were transfected with SETD1A  F2 alanine mutant constructs. Myc-DDK-tagged SETD1A  was used for Co-IP."
NCBI gene IDs:
"DDK: 
Myc: 
SETD1A: 9739"
Figure legend: "293T  cells were transfected with BuGZ  mutants. Myc-DDK-tagged BuGZ  mutants illustrated on Figure S5B were used for Co-IP."
NCBI gene IDs:
"DDK: 
Myc: 
BuGZ: 7756"
Figure legend: "293T  cells were transfected with BUB3  mutants and protein extracts were used for the  Co-IP experiments. Myc-DDK-tagged BUB3  mutants illustrated on Figure S5C were used."
NCBI gene IDs:
"DDK: 
Myc: 
BUB3: 9184"
Figure legend: "293T  cells were transfected with Myc-DDK-tagged human BuGZ  constructs and the protein extracts were used for a Co-IP  experiment."
NCBI gene IDs:
"DDK: 
Myc: 
BuGZ: 7756"
Figure legend: "293T  cells were transfected with Myc-DDK-tagged BuGZ  variant constructs and the protein extracts were used for the Co-IP  experiment."
NCBI gene IDs:
"DDK: 
Myc: 
BuGZ: 7756"
Figure legend: "293T  cells were transfected with Myc-DDK-tagged BuGZ  constructs and the protein extracts were used for a Co-IP experiment."
NCBI gene IDs:
"DDK: 
Myc: 
BuGZ: 7756"
Figure legend: "293T  cells were transfected with Myc-DDK-tagged wild-type BuGZ  and the cells were treated with 1,6-hexanediol for 3 h. The protein extracts were used for the Co-IP experiments."
NCBI gene IDs:
"DDK: 
Myc: 
BuGZ: 7756"
Figure legend: "SETD1A-GFP  fusion-expressing Setd1afl/fl;CreER MLL-AF9 leukemia cells were treated with tamoxifen, then assayed for  colony-forming potential. The FUCCI reporter (mCherry-Cdt1(30-120)/Citrine-Geminin(1-110))-expressing cells were used as control. Data from 3 biological replicates are shown. Data are presented as mean ± SD."
NCBI gene IDs:
"Cdt1: 
Citrine: 
Geminin: 
GFP: 
mCherry: 
Cre: 2777477
ER: 13982
MLL: 214162
AF9: 70122
SETD1A: 9739
Setd1a: 233904"
Figure legend: "SGC-expressing Setd1afl/fl;CreER MLL-AF9 leukemia  cells were treated with tamoxifen, and RNA-seq  analysis was performed with sorted cells. Data are shown as log2 fold change over tamoxifen  non-treated GFP-expressing control."
NCBI gene IDs:
"GFP: 
Cre: 2777477
ER: 13982
MLL: 214162
AF9: 70122
Setd1a: 233904"
Figure legend: " C Heatmap of the significant regulations upon induction of overexpression of the five members of the core network, the activators (green) ERF6, ERF59, ERF98 and the repressors (red) ERF8 and ERF9. Color code represents FDR-corrected p-values with thresholds at FDR=0.01, 0.05 and 0.1. Data information: In A, data are extrapolated from estimated averages, n = 3 independent experiments, FDR-corrected p-values &lt; 0.1 (mixed model analysis, user-defined Wald tests). The thickness of the arrows represents the FDR value. In B, data are presented as averages, n = 3 independent experiments. The intensity of the color of the arrows represents the strength of the regulation according to the TEA values and the thickness the FDR value of the nCounter Nanostring experiment. In C, data are represented as FDR-corrected p-values, n = 3 independent experiments. "
NCBI gene IDs:
"ERF6: 827463
ERF8: 841751
ERF9: 834444
ERF59: 844143
ERF98: 821901"
Figure legend: "B Average area of the individual leaves of rap2.6L and myb51, two knock-out lines with a smaller average rosette area."
NCBI gene IDs:
"myb51: 838438
rap2.6L: 831174"
Figure legend: " C The effect of ERF6, ERF9, ERF98 and WRKY15 on the GA2-OX6 promoter determined with transient expression assays. The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Data information: In C, data are presented as mean ± SEM, n = 3 independent experiments."
NCBI gene IDs:
"ERF6: 827463
ERF9: 834444
GA2-OX6: 839508
ERF98: 821901
WRKY15: 816864"
Figure legend: "B Leaf series of stz and erf11 mutants grown on control and mild osmotic stress conditions.Data information: In B, data are presented as mean ± SEM, n = 3 independent experiments, *=P&lt;0.05 (mixed model, partial F-tests)."
NCBI gene IDs:
"erf11: 839733
stz: 839666"
Figure legend: "A, B, C, D, E The effect of the individual and the combination of two TFs on the expression of target genes ERF11 (A) The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Green represents activation, red repression and gray absence of regulation. Data information: Data are presented as mean ± SEM, n = 3 independent experiments. "
NCBI gene IDs:
"ERF11: 839733"
Figure legend: "A, B, C, D, E The effect of the individual and the combination of two TFs on the expression of target genes ERF5 (B) The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Green represents activation, red repression and gray absence of regulation. Data information: Data are presented as mean ± SEM, n = 3 independent experiments. "
NCBI gene IDs:
"ERF5: 834770"
Figure legend: "A, B, C, D, E The effect of the individual and the combination of two TFs on the expression of target genes MYB51 (C) The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Green represents activation, red repression and gray absence of regulation. Data information: Data are presented as mean ± SEM, n = 3 independent experiments. "
NCBI gene IDs:
"MYB51: 838438"
Figure legend: "A, B, C, D, E The effect of the individual and the combination of two TFs on the expression of target genes, RAP2.6L (D) The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Green represents activation, red repression and gray absence of regulation. Data information: Data are presented as mean ± SEM, n = 3 independent experiments. "
NCBI gene IDs:
"RAP2.6L: 831174"
Figure legend: "A, B, C, D, E The effect of the individual and the combination of two TFs on the expression of target genes ERF6 (E). The relative luminescence was calculated relative to the control, 35S::GUS (n = 4 biological repeats). Green represents activation, red repression and gray absence of regulation. Data information: Data are presented as mean ± SEM, n = 3 independent experiments. "
NCBI gene IDs:
"ERF6: 827463"
Figure legend: "Cells were treated with CTRL, Beclin1 (left) or Atg7 (right) siRNA, then infected for 24 h. Cell mortality (that is, fold change relative to mock‐infected cells) (J), viral replication (K) and production (L) were assessed and whole‐cell lysates of siRNA‐treated cells were immunoblotted for Beclin1, Atg7, capsid and actin (M). Images and graphs shown are representative of at least three independent experiments and data presented in graphs correspond to mean+s.d. (n=3). Scale bar, 10 μm. CHIKV, Chikungunya virus; CTRL, control; GFP, green fluorescent protein; DMSO, dimethylsulphoxide; siRNA, short interfering RNA."
NCBI gene IDs:
"Atg7: 10533
Beclin1: 8678"
Figure legend: "Cells were treated with CTRL or p62 siRNA, then infected for 24 h. Cell mortality (that is, fold change relative to mock‐infected cells) (F), viral replication (left) and qRT‐PCR (right) (G), and viral production (H) were assessed and whole‐cell lysates were immunoblotted for p62, capsid or actin (I)."
NCBI gene IDs:
"p62: 8878"
Figure legend: "Cells were treated with CTRL or NDP52 siRNA, then infected for 24 h. Cell mortality (that is, fold change relative to mock‐infected cells) (J), viral replication (left) and qRT‐PCR (right) (K) and production were assessed (L)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "Whole‐cell lysates of siRNA‐treated cells were immunoblotted for NDP52, capsid or actin (M). Images and graphs shown are representative of at least three independent experiments and data presented in graphs correspond to mean+s.d. (n=3). Scale bar, 10 μm. C, capsid; CHIKV, Chikungunya virus; CTRL, control; NDP52, nuclear dot protein 52 kDa; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; siRNA, short interfering RNA."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "Cells were infected for 3 h and then transfected with empty plasmid (CTRL) or p62WT‐3XFLAG or p62deltaUBA‐3XFLAG. Immunoprecipitation experiments were performed 24 h p.i. with anti‐FLAG antibody. Immunoprecipitated proteins were revealed using antibodies to FLAG or capsid (F)."
NCBI gene IDs:
"p62: 8878"
Figure legend: "Cells were treated with CTRL or p62 siRNA, then infected for 24 h. Immunoprecipitation experiments were performed using antibodies to FK2 or nonspecific IgG controls. Immunoprecipitated proteins were revealed using anti‐capsid antibody (G)."
NCBI gene IDs:
"p62: 8878"
Figure legend: "Cells were treated with CTRL or p62 siRNA and transfected with NT or capsid‐3XFLAG. Cell mortality (that is, fold change relative to mock‐infected cells) was measured 48 h post transfection (L)."
NCBI gene IDs:
"p62: 8878"
Figure legend: "Cells were infected with CHIKV for 3 h and transfected with empty plasmid (CTRL), p62WT‐3XFLAG, p62deltaUBA‐3XFLAG or p62deltaLIR‐3XFLAG. Cell mortality (that is, fold change relative to mock‐infected cells) was measured 24 h post transfection (M)."
NCBI gene IDs:
"p62: 8878"
Figure legend: "Human NDP52 binds nsP2, localizes near CHIKV RCs, and restricts cell shutoff promoting cell survival in HeLa cells. Yeast cells expressing Gal4 DNA BD fused alone (empty vector) or to nsP2 were cotransformed with a plasmid encoding the Gal4 AD fused to NDP52 or the indicated NDP52 deletion mutants (top). Yeast cells expressing BD fused to nsP2 or the indicated nsP2 deletion mutants were cotransformed with a plasmid encoding the Gal4 AD fused alone (empty vector) or to NDP52 (bottom) (A)."
NCBI gene IDs:
"NDP52: 10241
Gal4: 855828
nsP2: 956309"
Figure legend: "Cells were treated with control (CTRL) (left) or NDP52 (right) siRNA, infected for 15 h, then labeled using antibodies to NDP52, nsP2 and puromycin. Quantitative analysis was performed by counting the percentage of infected cells with TGN‐associated RCs (n=30 cells per experiment) (D)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "Cells were transfected with empty plasmid (CTRL) or nsP2wt‐3XFLAG or nsP2R606A‐3XFLAG. Cell mortality (that is, % of dead cells) was measured 24 h post transfection (E)."
NCBI gene IDs:
"nsP2: 956309"
Figure legend: "Cells were treated with control (CTRL) or NDP52 siRNA, then mock infected (M) or infected (C) for 24 h. Cytoplasmic and nuclear fractions were isolated and immunoblotted for nsP2 and NDP52. The amounts of the cytoplasmic and nuclear fractions of nsP2 were quantified by densitometry and expressed as the ratio of nuclear to cytoplasmic fraction of nsP2 (F)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "Cells were treated with control (CTRL) or NDP52 siRNA, infected for 15 and 24 h and treated with puromycin. Whole‐cell lysates of siRNA‐treated cells were immunoblotted for NDP52, capsid or actin. Puromycin incorporation into newly synthesized protein was revealed using antibodies to puromycin (G)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "Cells were treated with CTRL or NDP52 siRNA, then transfected with NT or nsP2‐3XFLAG. Cell mortality (that is, fold change relative to mock‐infected cells) was measured 48 h post transfection (H)."
NCBI gene IDs:
"NDP52: 10241
nsP2: 956309"
Figure legend: "NDP52 promotes CHIKV infection in a species‐specific manner. MEF cells were treated with CTRL or NDP52 siRNA, then infected for 24 h. Cell mortality (that is, fold change relative to mock‐infected cells) (A) and viral production (B) were measured. Yeast cells expressing Gal4 DNA BD fused alone (empty vector) or to nsP2 were cotransformed with a plasmid encoding the Gal4 AD fused to hNDP52 or mouse NDP52 (C)."
NCBI gene IDs:
"NDP52: 10241
NDP52: 76815
Gal4: 855828"
Figure legend: "MEF cells were infected for 3 h and transfected with empty plasmid (CTRL) or hNDP52‐3XFLAG. Immunoprecipitation experiments were performed 24 h p.i. using antibodies to FLAG or nonspecific IgG controls. Immunoprecipitated proteins were revealed with anti‐nsP2 antibody (D)."
NCBI gene IDs:
"NDP52: 10241"
Figure legend: "MEF cells were infected for 3 h and transfected with empty plasmid (CTRL), hNDP52, hLC3‐C or hNDP52 and hLC3‐C. Viral production (E) and cell mortality (F) were assessed 24 h post transfection. Isogenic wt or Atg5−/− MEFs were infected for 24 h and cell mortality (that is, fold change relative to mock‐infected cells) (G) and viral production (H) were assessed. MEF cells were infected and immunoprecipitation experiments were performed 24 h p.i. using antibodies to p62 or nonspecific IgG controls. Immunoprecipitated proteins were revealed with anti‐capsid antibody (I)."
NCBI gene IDs:
"Atg5: 11793
NDP52: 10241
LC3‐C: 440738"
Figure legend: "MEF cells were treated with CTRL or p62 siRNA, then infected for 24 h. Cell mortality (that is, fold change relative to mock‐infected cells) (J) and viral production were assessed. Whole‐cell lysates of siRNA‐treated cells were immunoblotted for p62 or actin (K)."
NCBI gene IDs:
"p62: 18412"
Figure legend: "Quantitative RT-PCR and immunoblots of the expression of CD38 and SARM1 in DC and age-matched health control fibroblasts. All values are presented as mean ± SD of three replicates in quantitative RT-PCR. Protein levels are normalized to GAPDH, and mean (± SD) quantification values of 4 controls vs 5 DC samples are shown. Student's t test was performed on DC cells vs controls. Irrelevant intervening lanes have been removed for clarity (full blots are available online as Source Data)."
NCBI gene IDs:
"CD38: 952
SARM1: 23098"
Figure legend: "The efficacy of CD38 shRNA (sh-1 and sh-2) was verified by quantitative RT-PCR in DC fibroblasts and by RT-PCR and immunoblots in A549 cells. The relative CD38 mRNA values in the CD38 knockdown cells were normalized to the scrambled shRNA. All values are presented as mean ± SD of three replicates. Student's t test was performed on DC and A549 cells in indicated conditions."
NCBI gene IDs:
"CD38: 952"
Figure legend: "Intracellular NAD levels in the scramble and CD38 knockdown DC fibroblasts. The CD38 knockdown DC fibroblasts were treated with the PARP1 inhibitor Olaparib (400nM) and the SIRT1 inhibitor EX 527 (1 μM) for 24 hours. Data are representative four replicates. All values are presented as mean ± SD of four replicates. One-way ANOVA was performed on DC cells in indicated conditions."
NCBI gene IDs:
"CD38: 952"
Figure legend: "immunoblots of the expression of indicated proteins in the scramble and CD38 knockdown DC fibroblasts. The CD38 knockdown DC fibroblasts were treated with the PARP1 inhibitor Olaparib (400nM) and the SIRT1 inhibitor EX 527 (1 μM) for 24 hours. Data are representative four replicates. All values are presented as mean ± SD of four replicates. One-way ANOVA was performed on DC cells in indicated conditions."
NCBI gene IDs:
"CD38: 952"
Figure legend: "Intracellular NAD levels in brain tissues from 6-month- old G1 and G3 Tert-/- female mice."
NCBI gene IDs:
"Tert: 21752"
Figure legend: "immunoblots of the expression of NAD consuming related proteins and their activities in brain tissues from 6-month- old G1 and G3 Tert-/- female mice. The CD38 antibody was validated using the CD38-/- mouse brain tissues."
NCBI gene IDs:
"CD38: 12494
Tert: 21752"
Figure legend: "C Quantification of immunoblots from the indicated proteins in B. Data information: All values are presented as mean ± SD of G1 Tert-/- mouse brain tissues versus G3 Tert-/- mouse brain tissues. n=4 in each group. P values on the basis of Student's t test."
NCBI gene IDs:
"Tert: 21752"
Figure legend: "D NADase activity in G1 and G3 Tert-/- mouse brain lysate was determined at various time points during the reaction. NADase activity was not detectable in the extracts treated with the CD38 inhibitor, 78c, at a concentration of 50nM."
NCBI gene IDs:
"Tert: 21752"
Figure legend: "Cellular and mitochondrial ROS in DC and age-matched healthy control cells in the scramble and CD38 knockdown DC cells (D) were measured by flow cytometry from three replicates."
NCBI gene IDs:
"CD38: 952"
Figure legend: "A PCR amplification efficiency at the telomere in the mock- and FPG-treated DC and age-matched healthy control fibroblasts supplemented with vehicle or 3 mM NR. All values are presented as mean ± SD of three replicates. The relative telomere PCR amplification in each sample was normalized to the 36B4 reference gene."
NCBI gene IDs:
"36B4: "
Figure legend: "A Cumulative population doubling analysis of the proliferation of representative scramble and CD38 knockdown DC fibroblasts or DC fibroblasts treated with vehicle or 3 mM NR. Each data point is represented as mean ± SD of three replicates."
NCBI gene IDs:
"CD38: 952"
Figure legend: "(A) Kaplan Meier plot for survival in NeuT;Apln+/+ (n=11) and NeuT;Apln-/- (n=10) mice with mammary cancer after tumor onset. *P=0.0185; Log rank test."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(B) Tumor volumes, followed over time, of control mammary tumor E0771 cells (shRenilla) and Apelin-depleted (shApln) E0771 cells orthotopically-injected into both syngeneic C57BL/6J Apln+/+ and Apln-/- mice (5x105 cells/mouse), respectively. Tumor volumes were determined using calipers and are shown as mean tumor volumes ± S.E.M. Data shown is pooled from 2 independent experiments. Apln+/+;shRenilla (n=18), Apln+/+;shApln (n=17), Apln-/-;shRenilla (n=14), Apln-/-;shApln (n=15); ** P&lt;0.01, *** P&lt;0.001; two-way ANOVA."
NCBI gene IDs:
"Apelin: 30878
Apln: 30878"
Figure legend: "(C) Mean percentages (± S.E.M.) of CD31+ area in E0771 shRenilla (n=3) or shApln (n=3) mammary tumors, assessed on day 23 post-orthotopic injection into C57BL/6J Apln+/+ or Apln-/- mice, respectively. **P&lt;0.01; t test. (D) Mean percentages (± S.E.M.) of extravasated Dextran in E0771 shRenilla (n=9) or shApln (n=12) mammary tumors, assessed on day 19 post-orthotopic injection into C57BL/6J Apln+/+ or Apln-/- mice, respectively. **P&lt;0.01; t test. Right panel shows representative immunofluorescence of Dextran (red), CD31+ vessels (green), and DAPI (blue). The white arrows indicate regions of Dextran extravasation. Scale bars = 100 μm"
NCBI gene IDs:
"Apln: 30878"
Figure legend: "(E) Mean counts (± S.E.M.) of pimonidazole positive foci, assessed on day 26 post-orthotopic injection of E0771 shRenilla (n=6) or shApln (n=4) into C57BL/6J wild-type mice (5x105 cells/mouse). *P&lt;0.05; t test. Right panels show representative immunohistochemical pimonidazole staining at two different magnifications; Scale bars = 200 μm (upper panels) and 50 μm (lower panels)."
NCBI gene IDs:
"Apln: 30878"
Figure legend: "(F) Mean percentage (± S.E.M.) of tumor-infiltrating immune cells normalized to CD45+. E0771 shRenilla (n=8) or shApln (n=6) were orthotopically injected into C57BL/6J Apln+/+ or Apln-/- mice (5x105 cells/mouse), respectively and tumors were harvested day 25 post-injection. *P&lt;0.05, **P&lt;0.01; t test. All immune cell populations were gated for viable CD45+ cells and then further defined as: CD8 T cells (Thy1.2+, CD8+), CD4 T cells, (Thy1.2+, CD4+), Inflammatory Monocytes (Ly6C+, Cd11b+, Ly6G-), PMN-MDSCs (Ly6G+, Cd11b+), natural killer cells (Thy1.2-, Ly6G-, NK1.1+), natural killer T cells (Thy1.2+, CD4-, CD8-, NK1.1+) and peripheral dendritic cells (Ly6G- ,Ly6C+, Cd11b- , PDAC1+, B220+)."
NCBI gene IDs:
"Apln: 30878"
Figure legend: "(A) Left - Heatmap of RNA-Seq transcriptome analysis of CD31+/CD105+ endothelial cells sorted from tumors established by E0771 shRenilla (n=6) or shApln (n=3) cells orthotopically injected into C57BL/6J Apln+/+ or Apln-/- mice, respectively. Tumors were harvested day 25 post-injection and genes displayed are significantly deregulated at the adjusted p-value cut-off of 0.05. Right - Ingenuity Pathway Analysis for biological processes predicted to be decreased downstream of the differentially expressed genes."
NCBI gene IDs:
"Apln: 30878"
Figure legend: "(B) Quantification of vessel sprouts (mean values ± S.E.M.) upon VEGF treatment (30ng/mL) of embryoid bodies (EB) derived from murine ES cells (mESCs) with sense integrations in the Apelin gene (Apln STOP) of the splice acceptor described in Appendix Figure S1B or Cre-reverted antisense (Apln GO) sister cells. Apln GO (Day 2 n=6; Day 4 n=40, Day 6 n=54, Day 8 n= 32, Day 10 n=35, Day 11 n=31), Apln STOP (Day 2 n=4; Day 4 n=15, Day 6 n=31, Day 8 n= 28, Day 10 n=32, Day 11 n=37); ** P&lt;0.01, *** P&lt;0.001, two-way ANOVA. Representative brightfield images and automated analysis of vessel sprouts by Definiens software are shown in the bottom panels. Scale bars = 200 μm"
NCBI gene IDs:
"Apln: 30878
Apelin: 30878
Cre: 2777477"
Figure legend: "(C) Differentially expressed genes using RNAseq transcriptome analysis of CD31+ endothelial cells (ECs) sorted from sprouting EBs from Apelin STOP cells stimulated with VEGF (30ng/mL) and DMSO (-Apln) and repaired Apelin GO sister cells stimulated with VEGF and Apelin (1000nM) (+Apln). VEGF target genes and angiogenesis-related genes, predicted by Ingenuity Pathway Analysis (IPA) software, are indicated by bars on the upper axis of the heatmap. GO terms were analyzed by DAVID on-line software."
NCBI gene IDs:
"Apelin: 30878
Apln: 30878"
Figure legend: "(A) Experimental set up and (right) Kaplan Meier survival plot of NeuT;Apln+/+ and NeuT;Apln-/- mice with mammary cancer, left untreated (control) or treated with the indicate dose of the broad VEGFR blocker sunitinib. NeuT;Apln+/+ Control (n=8), NeuT;Apln-/- Control (n=11), NeuT;Apln+/+ Sunitinib (n=11), NeuT;Apln-/- Sunitinib (n=12); *P&lt;0.05; Log rank test. Mice were sacrificed when the tumor size reached 1cm3, following ethical guidelines. The dotted line indicates 50% of survival."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"
Figure legend: "(B) Percentages (mean ± S.E.M.) of tumor burden in mammary glands of untreated (control) and sunitinib-treated NeuT;Apln+/+ and NeuT;Apln-/- mice, assessed 4 weeks after tumor onset. NeuT;Apln+/+ Control (n=6), NeuT;Apln-/- Control (n=8), NeuT;Apln+/+ Sunitinib (n=5), NeuT;Apln-/- Sunitinib (n=5); *P&lt;0.05; ***P&lt;0.001; one-way ANOVA. Representative H&amp;E images are shown for each genotype in the right panels. Scale bars = 1000 μm (large panels) and 50 μm (insets)."
NCBI gene IDs:
"Apln: 30878
Neu: 13866"