EMBO/SourceData huggingface dataset:
Figure legend: "(E-F) M238P cells were transfected with two different sequences of siRNAs vs FSCN1 or with a control siRNA (72 h, 100 nM). (E) Images and quantification of cell area in cells stained  for  F-actin (red) and nuclei  (blue). Data is represented as scatter plot with mean ± SD (n≥30 cells per condition). Mann-Whitney U test was used for statistical analysis. ****P≤0.0001. Scale bar 40 μm. (F) Images and quantification of  focal adhesions (FA) number in cells  stained  for pPaxillin  (green) and nuclei  (blue). Focal adhesions number is represented as mean ± SD (n≥30 cells per condition). Each point represents the average number of focal adhesions per cell calculated for each field. Paired Student t-test was used for statistical analysis. ****P≤0.0001. Scale bar 40 μm."
NCBI gene IDs:
"FSCN1: 6624"
Figure legend: "E) M238P cells were transfected with miR-143-3p, miR-145-5p or a control mimic (miR-neg) (72 h, 30 nM). (E) Effect of miR-143-3p or miR-145-5p overexpression on the  expression of YAP/MRTF target genes assessed by RT-qPCR. Data are normalized to the  expression in control cells. Data is represented as mean ± SD from a triplicate representative of at least 3 independent experiments. Paired Student t-test was used for statistical analysis. *P≤0.05, **P≤0.01, ****P≤0.0001."
NCBI gene IDs:
"miR-143-3p: 406935
miR-145-5p: 406937"
Figure legend: "M238P cells were transfected with two different sequences of siRNAs vs FSCN1  or with a control siRNA (72 h, 100 nM). Effect of FSCN1 downregulation on MRTFA (F) and YAP1 (G) nuclear translocation assessed by  immunofluorescence  in M238P. Data are represented as scatter plot with mean ± SD (n≥30 cells per condition). Each point represents the nuclear/cytoplasm ratio. Mann-Whitney U test was used for statistical analysis. ***P≤0.001, ****P≤0.0001. Scale bar 40 μm."
NCBI gene IDs:
"FSCN1: 6624"
Figure legend: "M238P cells were transfected with two different sequences of siRNAs vs  FSCN1 or with a control siRNA (72 h, 100 nM). (H) RT-qPCR  analysis for the expression of MRTFA/YAP target genes in M238P cells transfected with the indicated siRNAs. Data are normalized to the  expression in parental cells. Data is represented as mean ± SE from a triplicate representative of at least 3 independent experiments. Paired Student t-test was used for statistical analysis. *P≤0.05, **P≤0.01, ***P≤0.001. Scale bar 40 μm."
NCBI gene IDs:
"FSCN1: 6624"
Figure legend: " A) Treadmill analysis (n=8/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by unpaired, two-tailed Student's t test ***p=0.005; ***p=0.0007 (T3) and ****p&lt;0.0001 (T4). Only p values for rapamycin-treated vs. untreated Cox15sm/sm mice are shown "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " B) Histological and histochemical characterization of skeletal muscle in rapamycin-treated and untreated Cox15sm/sm and WT mice. H&amp;E: hematoxylin and eosin; PAS: periodic acid Schiff reaction; COX: cytochrome c oxidase; SDH: succinate dehydrogenase. White bars correspond to 50µm "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " C) Analysis of the cross-sectional area of muscle fibers in the different genotypes (n=3/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test: ***p=0.001; ***p=0.006, ****p&lt;0.0001 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " D) Analysis of the number of centralized nuclei in muscle fibers (n=3/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test **p=0.0018, **p=0.0052 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " E) Analysis of PAS-reaction intensity in muscle fibers (n=4/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test *p=0.0293 (WT vs. Cox15sm/sm) and *p=0.0150 (Cox15sm/sm vs. Cox15sm/sm Rapa) "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " F) Analysis of COX-reaction intensity in muscle fibers (n=3). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test ****p&lt;0.0001; **p=0.0031 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " G) Analysis of SDH-reaction intensity in muscle fibers (n=3). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test *p=0.0199, **p=0.0091 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " A) Spectrophotometric activities of the respiratory chain (n=4-5/group). CS: citrate synthase; CIV: complex IV. Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by two way ANOVA with Tukey's correction ****p&lt;0.0001 (CS: WT vs. Cox15sm/sm), ***p=0.0010 (CS: Cox15sm/sm vs. Cox15sm/sm rapamycin), ****p&lt;0.0001 (CIV: WT vs. Cox15sm/sm), **p=0.0060 (CIV/CS: WT vs. Cox15sm/sm) "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " B) Western blot immunovisualization of subunits of the respiratory complexes. Note the increased protein levels in Cox15sm/sm vs. WT samples, which are reduced to normal levels upon rapamycin treatment. Additional samples were run on a separate gel (not shown) "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " C) BNGE in gel activity for cIV. Sc: supercomplexes. Note that the COX reaction is slightly increased in rapamycin-treated vs. untreated Cox15sm/sm samples "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " D) Immunoblot of 1D-BNGE using an anti-cIV antibody (COX1). Note that COX amount is slightly increased in rapamycin treated vs. untreated Cox15sm/sm muscles. SDHB was used as a loading control "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " E) State III (succinate-driven) oxygen consumption (n=3/group). Error bars represent SEM. The asterisks represent the significance levels calculated by unpaired, two tail Student's t test **p=0.0016 (WT vs. Cox15sm/sm); *p=0.047 (Cox15sm/sm vs. Cox15sm/sm rapamycin) "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " Note the accumulation of profoundly altered mitochondria in Cox15sm/sm muscles (white arrows), possibly as a result of partially digested organelles within endolysosomes. Black arrows indicate examples of normal mitochondria. In rapamycin-treated Cox15sm/sm animals only a few of these structures are present, which can be detected also in rapamycin-treated WT samples. The bar corresponds to 1136 nm (4400X) "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " A) Representative western blot of autophagy markers at steady state in skeletal muscle. GAPDH was used as a loading control. B) Densitometric analysis of immunoblots similar to that shown in A (n=4/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test **p=0.0068 (LC3-II WT vs. Cox15sm/sm), **p=0.0063 (P62 WT vs. Cox15sm/sm) *p=0.025 (P62 Cox15sm/sm vs. Cox15sm/sm+rapamycin), **p=0.0098 (S6 WT vs. Cox15sm/sm), *p=0.034 (P-S6 WT vs WT+rapamycin), ***p=0.0002 (Cox15sm/sm vs. Cox15sm/sm+rapamycin), *p=0.014 (P-S6 WT vs. WT+rapamycin) ***p=0.0007 (Cox15sm/sm vs. Cox15sm/sm+rapamycin)"
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " C) Analysis of the autophagic flux (n=3/group). Upper panel: representative western blot of Cox15sm/sm and WT muscles treated with colchicine, rapamycin, or rapamycin plus colchicine. Note that LC3-II was increased in Cox15sm/sm vs. WT samples. Colchicine did not increase LC3-II levels suggesting a block in the autophagic flux; rapamycin plus colchicine treated Cox15sm/sm mice showed higher levels of LC3-II, suggesting that rapamycin increased the autophagic flux in Cox15sm/sm muscles. Black: WT; dark grey: colchicine-treated WT; middle grey: rapamycin-treated WT; light grey: colchicine plus rapamycin treated mice; red: untreated Cox15sm/sm; orange: colchicine-treated; blue: rapamycin-treated Cox15sm/sm; purple: colchicine plus rapamycin treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test **p=0.0049 (WT vs. WT+colchicine), **p=0.0046 (WT+rapamycin vs. WT+rapamycin+colchicine), *p=0.0225 (WT vs. Cox15sm/sm), **p=0.0057 (Cox15sm/sm colchicine vs. Cox15sm/sm+rapamycin+colchicine), ***p=0.0003 (Cox15sm/sm rapamycin vs. Cox15sm/sm+rapamycin+colchicine)"
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " A) Analysis of the autophagic flux (n=4/group). Upper panel: representative western blot of Cox15sm/sm and WT muscles treated with colchicine, rilmenidine, or rilmenidine plus colchicine. Colchicine alone increased LC3-II in WT but not Cox15sm/sm mice; rilmenidine had no effect on WT LC3-II levels, but reduced them in Cox15sm/sm mice. Rilmenidine plus colchicine increased LC3-II levels in both WT and Cox15sm/sm mice. Black: WT; dark grey: colchicine-treated WT; middle grey: rapamycin-treated WT; light grey: colchicine plus rapamycin treated mice; red: untreated Cox15sm/sm; orange: colchicine-treated; blue: rapamycin-treated Cox15sm/sm; purple: colchicine plus rapamycin treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test: **p=0.0093 (WT vs. WT+colchicine), ****p&lt;0.0001 (WT+rilmenidine vs. WT+rilmenidine+colchicine), **p=0.0001 (WT vs. Cox15sm/sm), **p=0.0083 (Cox15sm/sm vs. Cox15sm/sm+rilmenidine), *p=0.0112 (Cox15sm/sm vs. Cox15sm/sm+rilmenidine+colchicine), ****p&lt;0.000"
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " B) Treadmill analysis (n=4/group). Black: untreated WT; grey: rilmenidine-treated WT; red: untreated Cox15sm/sm; blue: rilmenidine-treated Cox15sm/sm. Error bars represent SEM "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " C) Histological and histochemical characterization of skeletal muscle in rapamycin-treated and untreated Cox15sm/sm and WT mice. H&amp;E: hematoxylin and eosin; GT: Gomori trichrome; PAS: periodic acid Schiff reaction "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " D) Analysis of the cross-sectional area of muscle fibers in the different genotypes (n=3/group). Black: untreated WT; grey: rilmenidine-treated WT; red: untreated Cox15sm/sm; blue: rilmenidine-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test ***p=0.0002, ****p&lt;0.0001 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " E) Analysis of the number of centralized nuclei in muscle fibres (n=3/group). Black: untreated WT; grey: rilmenidine-treated WT; red: untreated Cox15sm/sm; blue: rilmenidine-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test **p=0.0091 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " A) anti-TFEB immunofluorescence on rapamycin- and rilmenidine-treated WT and Cox15sm/sm muscles vs. untreated (UT) samples. CD11C (blue signal) indicates inflammatory cells. Right panel: quantification of n=3 animals/group. The scale bars correspond to 30µm. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test B) Quantification of the TFEB-positive nuclei (n=3/group). Quantification was performed using Imaris spots surface excluding the CD11C positive nuclei. Error bars represent SEM. The asterisks represent the significance levels calculated by one-way ANOVA with Tukey post-hoc multiple comparison test *p=0.0199 (WT vs. WT+rapamycin), *p=0.0150 (Cox15sm/sm vs. Cox15sm/sm+rapamycin), ***p=0.0001 "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: " A) Real-time PCR analysis of Lamp1 transcript (n=3/group). Black: untreated WT; grey: rapamycin-treated WT; red: untreated Cox15sm/sm; blue: rapamycin-treated Cox15sm/sm. Error bars represent SEM. The asterisks represent the significance levels calculated by Student's t test **p=0.008, *p=0.038 "
NCBI gene IDs:
"Cox15: 226139
Lamp1: 16783"
Figure legend: " B) Anti-LAMP1 staining in rapamycin- and rilmenidine-treated WT and Cox15sm/sm muscles vs. untreated (UT) samples. Rapamycin, but not rilmendine, increases the number of LAMP1-positive vesicles in Cox15sm/sm samples (inset). The scale bars correspond to 30µm "
NCBI gene IDs:
"Cox15: 226139"
Figure legend: "D. mRNA expression  of PGF2α  synthases in the retinas  of OIR mice  during postnatal days 12-17 (n= E. mRNA expression  of Ptgfr  in the retinas  of OIR mice  during postnatal days 12-17 (n=6-8). Data information: n.s. stands for \"not significant.\" Data were analyzed by , two-way ANOVA with Tukey's multiple comparisons test D, E, Data are represented as mean ± SEM."
NCBI gene IDs:
"Ptgfr: 19220"
Figure legend: "F. mRNA expression  of Ptgfr  in each retinal  layer  from normoxic and OIR mice  on postnatal day 16 (n=6). The left-hand image displays representative cross-sections from OIR and normoxic retinas, with  isolectin B4-stained vessels  in green, DAPI-stained nuclei  in blue, and LCM  isolation locations circled by white dashed circles. INL,  inner nuclear layer; ONL, outer nuclear layer; RGC,  retinal ganglion cells. Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test F) Scale bar: 20 μm. Data are represented as mean ± SEM."
NCBI gene IDs:
"Ptgfr: 19220"
Figure legend: "G. Ptgfr  mRNA expression  in retinal endothelial cells  (CD31+CD45-) sorted by MACS from normoxic and OIR mice  on postnatal day 16 (n=5). Data information: n.s. stands for \"not significant.\" Data were analyzed Mann-Whitney test (G). Scale bar: 20 μm. Data are represented as mean ± SEM."
NCBI gene IDs:
"Ptgfr: 19220"
Figure legend: "B. Ptgfr  mRNA levels in retinal microvessels   of CKO-T and control mice  (n=6). INL,  inner nuclear layer; ONL, outer nuclear layer; RGC, retinal ganglion cells. Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test (B, Data are represented as mean ± SEM."
NCBI gene IDs:
"Ptgfr: 19220"
Figure legend: "D. Effect of PTGFR  deficiency in ECs  on ELR+ CXC chemokine expression  in retinas  from OIR mice  on postnatal day 16 (n=6). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test image).Data are represented as mean ± SEM."
NCBI gene IDs:
"PTGFR: 19220"
Figure legend: "F. Retinal CXCL1 protein-expression levels in Cxcl1-expressing lentivirus-injected CKO mice  (n=3). G. Quantitation of retinal CXCL1  protein in F (n=3). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test G, image).Data are represented as mean ± SEM."
NCBI gene IDs:
"Cxcl1: 14825"
Figure legend: "H. Representative images of oxygen-induced retinal angiogenesis   in Cxcl1-expressing lentivirus-injected CKO mice  on postnatal day 17. Green represents the isolectin B4-stained vessels, the second-row panels display the enlarged images of white boxes in the first-row panels, the third-row images show  neovascular tufts (NV, white), and the fourth-row images show the vaso-obliteration (VO, white) area. I. Quantitation of oxygen-induced retinal neovascularization  in H (n=8). J. Quantitation of retinal vaso-obliteration  in H (n=8). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test , I, J). Scale bar: 500 μm (H, unmagnified image), 150 μm (H, magnified image).Data are represented as mean ± SEM."
NCBI gene IDs:
"Cxcl1: 14825"
Figure legend: "B. Relative expression  levels of FOS  in  retinal microvascular ECs from patients  with PDR  (n=4) compared with the corresponding expression  levels from normal subjects (n=7) (Gene Expression Omnibus Database dataset GSE94019). TPM stands for \"Transcripts per kilobase million.\" Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test (B, Data in B are expressed as the mean + SD."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "C. Effect of FOS  knockdown on 500 nM PGF2α-induced CXCL8 expression  in HRMECs  (n=4). D. Effect of FOS  knockdown on 500 nM PGF2α-induced HRMEC CXCL8  secretion in the culture medium (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test C, D, Data in C, D, are represented as mean ± SEM. "
NCBI gene IDs:
"CXCL8: 3576
FOS: 2353"
Figure legend: "E. and F. Effect of RA  (1 μM, AP-1  dimer inhibitor), SN-50  (100 μg/mL, NFκB  inhibitor), and  Inca-6  (2.5 μM, NFAT  inhibitor) on 500 nM PGF2α-induced CXCL8 expression  in HRMECs and HRMEC CXCL8  secretion in the culture medium (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test E, F, Data in E, F, are represented as mean ± SEM. "
NCBI gene IDs:
"CXCL8: 3576"
Figure legend: "G. Representative images of scratch-induced migration  of 500 nM PGF2α-treated HRMECs  with or without FOS  knockdown. The yellow solid line indicates the original position of the scratch, the dashed line indicates the position of the cells after migration, and the arrows represent the migratory direction. H. Quantitation of the cell-migration  distance in H (n=4). I. Effect of FOS  knockdown on 500 nM PGF2α-induced HRMEC proliferation  (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test (H, I Scale bar: 100 μm (G) Data in H, I, are represented as mean ± SEM. "
NCBI gene IDs:
"FOS: 2353"
Figure legend: "J. Representative images of tube  formation by 500 nM PGF2α-treated HRMECs  with or without FOS  knockdown. K. Quantitation of total tube  length, number of junctions, number of meshes, and percentage of mesh area in J (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test K, Scale bar: 200 μm (J, Data in K, are represented as mean ± SEM. "
NCBI gene IDs:
"FOS: 2353"
Figure legend: "L. Fos  mRNA levels in retinas  from OIR mice during postnatal days 12-17 (n=3-4). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test L, Data in , L, are represented as mean ± SEM. "
NCBI gene IDs:
"Fos: 14281"
Figure legend: "M. Effect of PTGFR  deficiency in ECs  on Fos expression  in the retina  from OIR mice  on postnatal day 16 (n=6). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test M, Data in M, are represented as mean ± SEM. "
NCBI gene IDs:
"Fos: 14281
PTGFR: 19220"
Figure legend: "B. Effect of U73122  (10 μM), BAPTA  (50 μM), Y27632  (5 μM), and Ly294002  (15 μM) treatment on 500 nM PGF2α-induced FOS gene expression in HRMECs  (n=4). C. Effect of KN93  (10 μM) and RO-318220 (250 nM) treatment on 500 nM PGF2α-induced FOS expression  in HRMECs  (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test (B, C Data are represented as mean ± SEM."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "D. Effect of SB203580  (2 μM), PD98059  (20 μM), and SP600125  (60 nM) treatment on 500 nM PGF2α-induced FOS gene expression in HRMECs  (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test Data are represented as mean ± SEM."
NCBI gene IDs:
"FOS: 2353"
Figure legend: "J. Effect of CAMK2G  or CAMK2D  knockdown on 500 nM PGF2α-triggered p38 phosphorylation  in HRMECs. K. Quantitation of the ratio of p38 phosphorylation  to total p38  protein in J (n=3). Data information: n.s. stands for \"not significant.\" Data were analyzed by unpaired student's t-test Data are represented as mean ± SEM."
NCBI gene IDs:
"CAMK2D: 817
CAMK2G: 818"
Figure legend: "L. Effect of CAMK2G  or CAMK2D  knockdown on 500 nM PGF2α-induced FOS expression  in HRMECs  (n=4). Data information: n.s. stands for \"not significant.\" Data were analyzed by Mann-Whitney test L) Data are represented as mean ± SEM."
NCBI gene IDs:
"CAMK2D: 817
CAMK2G: 818
FOS: 2353"
Figure legend: "B. Representative images of OIR retinas  in WT and Cxcr2-/- mice  with or without AL8810  treatment. The green color shows the  isolectin B4-stained vessels, the second-row panels display the enlarged images of white boxes in the first-row panels, the third-row images show  neovascular tufts (NV, white), and the fourth-row images show the  vaso-obliteration (VO, white) area. C. Quantitation of oxygen-induced retinal neovascularization  in WT and Cxcr2-/- mice  with or without AL8810  treatment (n=12). D. Quantitation of retinal vaso-obliteration in WT and Cxcr2-/- mice  with or without AL8810  treatment (n=12). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Tukey's multiple comparisons test (C, D) Scale bar: 500 μm (unmagnified image), 150 μm (magnified image). Data are represented as mean ± SEM."
NCBI gene IDs:
"Cxcr2: 12765"
Figure legend: "E. Effect of AL8810  on retinal Fos expression  in WT and Cxcr2-/- OIR mice  on postnatal day 16 (n=6). F. Effect of AL8810  on retinal Cxcl1 expression  in WT and Cxcr2-/- OIR mice  on postnatal day 16 (n=6). Data information: n.s. stands for \"not significant.\" Data were analyzed by two-way ANOVA with Mann-Whitney test (E, F). Data are represented as mean ± SEM."
NCBI gene IDs:
"Cxcl1: 14825
Cxcr2: 12765
Fos: 14281"
Figure legend: " A Neonatal rat ventricular myocytes (NRVMs) were infected with the 3xMEF2-Luc reporter, serum starved for 20 hours and stimulated for 24 hours with different agonists: 100 nM endothelin-1 (ET1), 1 μM sphingosine-1-phosphate (S1P), 10 μM lisophosphatidic acid (LPA), 1 μM WIN55,212-2 (WIN55, cannabinoid receptor agonist), 1 μM isoproterenol (ISO, β-adrenergic receptor agonist), 100 nM angiotensin II (AngII), 10 μM prostaglandin E1 (PGE1), 10 μM prostaglandin E2 (PGE2) or 100 nM prostanoid F receptor agonist fluprostenol (Flupro), 100 nM treprostinil (Trepro, prostacyclin receptor agonist) Data information: values are mean±s.e.m. In (A), the experiment was performed in triplicates, similar results were obtained in 3 different experiments. Student's two-tailed t-test, *,P&lt;0.05 vs. control (ET1, P=0.0013; S1P, P=0.0144; LPA, P=0.026; WIN55, P=0.6205; ISO=0.0399; AngII, P=0.5812; PGE1, P=0.001; PGE2, P&lt;0.0001; Flupro, P=0.0846; Trepro, P=0.1958) "
NCBI gene IDs:
"MEF2: 498607///81518///309957///499497"
Figure legend: " B PGE2 induces the expression of MEF2 target genes. NRVMs were serum-starved for 24 hours, then were stimulated with 1 μM PGE2 for 2 hours and mRNA levels of the indicated genes were determined Data information: values are mean±s.e.m In (B), n=5, technical replicates, Student's two-tailed t-test, *,P&lt;0.05 vs. control (Nur77, P=0.0148; Myomaxin, P=0.0417; Adamts1, P=0.0112; BNP, P=0.0065)"
NCBI gene IDs:
"Adamts1: 79252
BNP: 25105
Nur77: 79240
Myomaxin: 311098"