EMBO/SourceData huggingface dataset:
Figure legend: "A Layer V cortex explants from E18.5 NB-3+/+ and NB-3−/−mice were cultured in medium supplemented with either Fc or NB-3-Fc (50 μg/ml).B Quantification of neurite outgrowth in (A). n.s., not significant, **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. Data were analyzed from more than 50 layer V cortical explants from 5 independent experiments in each group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "C Pyramidal neurons of NB-3+/+ and NB-3−/− mice were cultured in medium supplemented with either Fc or NB-3-Fc (50 μg/ml). The neurons were co-stained for Ctip2, NB-3, and Rhodamine phalloidin. (C1-C4) High-magnification images of the growth cones (white arrowheads) of the neurons in (C), respectively.D Quantification of neurite length in (C), and the percentage of collapsed growth cones in (C). n.s., not significant, *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. Data were analyzed from more than 300 corticospinal neurons from 6 independent experiments in each group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "A Images of coronal sections of layer Vsensorimotor cortex from NB-3+/+ and NB-3−/−mice after SCI. Corticospinal neurons were retrogradely labeled with Fluorogold (FG), and coronal sections were co-stained with p-mTOR (A) (arrowheads).B Quantification of fluorescence intensity of p-mTOR in FG-labeled corticospinal neurons in (A). *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. The intensities of more than 300 corticospinal neurons from 3 mice in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "C Images of coronal sections of layer Vsensorimotor cortex from NB-3+/+ and NB-3−/−mice after SCI. Corticospinal neurons were retrogradely labeled with Fluorogold (FG), and coronal sections were co-stained with p-S6 (S240/244) (C) (arrowheads).D Quantification of fluorescence intensity of p-S6 (S240/244) in FG-labeled corticospinal neurons in (C). *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. The intensities of more than 300 corticospinal neurons from 3 mice in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "E-H Western blot analysis and quantification of the expression of p-Akt/Akt (E and F) and p-S6 (S240/244) (G and H) in sensorimotor cortex from NB-3+/+ and NB-3−/− mice after SCI. *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. Data were analyzed from 3 independent experiments each including 3 mice per group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "I, K, M, O Expression of Akt (I), p-Akt (K), p-mTOR (M), and p-S6 (S240/244) (O) in NB-3+/+ pyramidal neuron-astrocyte co-cultures. The astrocytes were transfected either with NB-3-Myc or a control vector. The dashed lines outline the astrocytes. The asterisks indicate the NB-3-expressing astrocytes.J, L, N, P Quantification of fluorescence intensity of Akt (J), p-Akt (L), p-mTOR (N), and p-S6 (S240/244) (P), respectively, in pyramidal neurons of the co-cultures in (I, K, M, and O). n.s., not significant, and *p &lt; 0.05; one sample t-test. The intensities of more than 300 pyramidal neurons from 6 independent experiments in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "A Co-immunostaining of CHL1 (blue), NB-3 (green), and BDA (red) in injured spinal cords of NB-3+/+ (A1) and NB-3−/− (A5) mice. (A2-A4 and A6-A8) High-magnification images of boxed areas in (A1 and A5), respectively. Images in (A2 and A6) are presented as single-channel fluorescence images in (A3-A4 and A7-A8), respectively.B Quantification of fluorescence intensity of CHL1 at the spinal lesion sites of NB-3+/+ and NB-3−/− mice after SCI. *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. n = 11 mice per group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "C, D Western blot analysis (C) and quantification (D) of CHL1 expression in spinal lesion areas of NB-3+/+ and NB-3−/− mice after SCI. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001; one-way ANOVA followed by Bonferroni post-test. Data were analyzed from 3 independent experiments each including 2 mice per group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "E Co-immunoprecipitation of NB-3 and CHL1 from spinal cord lesion areas of NB-3+/+ and NB-3−/−mice."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "F Co-immunostaining of CHL1 and NB-3 in FG-labeled corticospinal neurons (arrowheads) of NB-3+/+ and NB-3−/− mice 14 days post-injury (dpi).G Quantification of the fluorescence intensity of CHL1 in (F). *p &lt; 0.05, and **p &lt; 0.01; one-way ANOVA followed by Bonferroni post-test. The intensities of more than 300 corticospinal neurons from 3 mice in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "H, I Western blot analysis (H) and quantification (I) of CHL1 expression in sensorimotor cortices of NB-3+/+ and NB-3−/− mice after SCI. *p &lt; 0.05; one-way ANOVA followed by Bonferroni post-test. Data were analyzed from 3 independent experiments each including 3 mice per group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "J CHL1 expression in the somas and axons of NB-3+/+ and NB-3−/− pyramidal neurons when co-culturing with NB-3-expressing astrocytes (dashed lines and asterisks) or astrocytes that didn't express NB-3 (dashed lines). Co-localization of CHL1 and NB-3 in astrocytes overexpressed NB-3 (asterisks). Co-localization of CHL1 and NB-3 in the somas and axons of co-cultured NB-3+/+ pyramidal neurons (arrowheads). Left inset, high-magnification view of the soma (white arrowhead). Right inset, high-magnification view of the axon tip (green arrowhead).K Quantification of fluorescence intensity of CHL1 in (J). *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001; one-way ANOVA followed by Bonferroni post-test. The intensities of more than 300 astrocytes or pyramidal neurons from 3 independent experiments in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "L-N Sagittal sections showing the corticospinal axons in injured spinal cords following injection of LV-nc-GFP (L) and LV-CHL1 shRNA-GFP (M) into both the sensorimotor cortex and lesion sites. High-magnification image (N) of the area in (M) (asterisk). The arrows indicate the lesion sites; the asterisk indicates the lesion epicenter.O Quantification of intensity index of BDA-labeled axons at certain distances from the lesion border in (L and M). *p &lt; 0.05 and **p &lt; 0.01; two-way ANOVA followed by Fisher's LSD. n= 9 mice in (L), and n= 10 mice in (M)."
NCBI gene IDs:
"CHL1: 12661"
Figure legend: "A Co-immunostaining of PTPσ (blue), NB-3 (green), and BDA (red) at spinal lesion sites of NB-3+/+ (A1) and NB-3−/− (A6) mice. (A2-A5 and A7-A10) High-magnification images of boxed areas in (A1 and A6) showing PTPσ expression at the terminations of BDA-labeled corticospinal axons, respectively. Images in (A2 and A7) are presented as single-channel fluorescence images in (A3-A5 and A8-A10), respectively.B Quantification of fluorescence intensity of PTPσ at BDA-labeled axonal terminations of NB-3+/+ and NB-3−/− mice after SCI. *p &lt; 0.05; one-way ANOVA followed by Bonferroni post-test. n = 12 mice per group."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "C Co-immunostaining of PTPσ and NB-3 in the FG-labeled corticospinal neurons (arrowheads) of NB-3+/+ and NB-3−/− mice 14 days post-injury (dpi). Insets, only the signals from PTPσ and FG in NB-3+/+ corticospinal neurons (red arrowheads) were visualized.D Quantification of fluorescence intensity of PTPσ in (C). *p &lt; 0.05; one-way ANOVA followed by Bonferroni post-test. The intensities of more than 300 corticospinal neurons from 3 mice in each group were quantified."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "G PTPσ expression in NB-3+/+ and NB-3−/− pyramidal neurons when co-culturing with NB-3-expressing astrocytes (dashed lines and asterisks) or astrocytes that didn't express NB-3 (dashed lines). Upper inset, high-magnification view of the soma (white arrowhead). Lower inset, high-magnification view of the axon tip (green arrowhead).H Quantification of fluorescence intensity of PTPσ in (G). n.s., not significant, and *p &lt; 0.05; one-way ANOVA followed by Bonferroni post-test."
NCBI gene IDs:
"NB-3: 53870"
Figure legend: "I Expression of PTPσ, p-Akt, p-mTOR, and p-S6 (S240/244) in pyramidal neurons that expressed PTPσ shRNA-GFP. A control plasmid expressing GFP was transfected as the negative control (nc).J Quantification of fluorescence intensity of PTPσ, p-Akt, p-mTOR, and p-S6 (S240/244) in (I). *p &lt; 0.05; one sample t-test. The intensities of more than 300 pyramidal neurons from 4 independent experiments in each group in (H) and (J) were quantified, respectively."
NCBI gene IDs:
"PTPσ: 19280"
Figure legend: "K-M Sagittal sections showing the corticospinal axons in injured spinal cords following injection of LV-nc-GFP (K) and LV-PTPσ shRNA-GFP (L) into the sensorimotor cortex. High-magnification image (M) of the area in (L) (asterisk). The arrows indicate the lesion sites; the asterisk indicates the lesion epicenter.N Quantification of the intensity index of BDA-labeled axons at certain distances from the lesion border in (K and L). *p &lt; 0.01; two-way ANOVA followed by Fisher's LSD. n = 8 mice in (K), and n = 10 mice in (L)."
NCBI gene IDs:
"PTPσ: 19280"
Figure legend: "A-D The morphology of uninjected, FLT3/WT mRNA and FLT3/ITD mRNA injected (150 ng per embryo) embryos on day 2 post fertilization (dpf). Data information: ov: otic vesicles Scale bar = 500 μm."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "E-H Whole mount in situ hybridization (WISH) of notochord-specific marker col9a2 in uninjected, FLT3/WT mRNA and FLT3/ITD mRNA injected embryos on 2 dpf. Data information: ov: otic vesicles; cm: cephalic mesoderm; nc: notochord. Scale bar = 500 μm."
NCBI gene IDs:
"col9a2: 321212
FLT3: 2322"
Figure legend: "FLT3 signaling was detected by Western blotting in 293FT cells-transfected with FLT3/ITD mRNA (I)"
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "FLT3 signaling was detected by Western blotting in zebrafish embryos-injected with FLT3/ITD mRNA (J)."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "K The effect of FLT3 inhibitor quizartinib (Qui) on the dorsalization and axis-duplication phenotype-induced by FLT3/ITD mRNA injection in zebrafish. Data information: the experiments were performed in triplicates and the data are presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test). NS, not significant."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "Quantification of fst expression by RT-qPCR (L) after FLT3/ITD overexpression in zebrafish embryos at 6 hpf. Data information: the experiments were performed in triplicates and the data are presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test). NS, not significant."
NCBI gene IDs:
"FLT3: 2322
fst: 100004116"
Figure legend: "Quantification of fst expression by Western Blotting (M), after FLT3/ITD overexpression in zebrafish embryos at 6 hpf."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "Quantification of fst expression by WISH (N) after FLT3/ITD overexpression in zebrafish embryos at 6 hpf."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "A-C WISH of fst in FLT3/WT- (A), FLT3/ITD- plasmid DNA injected zebrafish embryos without (B) or with (C) quizartinib treatment (2.5 μM) from 6 to 36 hpf. fst expression was expanded by FLT3/ITD DNA in 86% of embryos (B, arrow, 32/37) which could be effectively blocked by treating with FLT3 inhibitor quizartinib in 83% of embryos (C, 29/35). Data information: Scale bar = 500 μm."
NCBI gene IDs:
"FLT3: 2322
fst: 100004116"
Figure legend: "Generation and characterization of FLT3/ITD-transgenic zebrafish. GFP expression was detected by florescent microscopy (F-H) and in blood circulation and thymus by WISH (I and J, blue arrow) in WT sibling and Runx1-FLT3/ITD transgenic zebrafish (F1) embryos at 4 dpf. FLT3/ITD-positive zebrafish (F1) were confirmed by PCR genotyping of GFP and FLT3/ITD using genomic DNA from fin clip of WT siblings and Runx1-FLT3/ITD transgenic zebrafish (F1) at two months old. Fish 4, 5 and 6 showed germline transmission of FLT3/ITD transgene (K). Data information: Scale bar = 500 μm."
NCBI gene IDs:
"GFP: 
FLT3: 2322
Runx1: 12394"
Figure legend: "Kidney marrow (KM) was collected from Runx1-FLT3/ITD transgenic zebrafish (F1) at 18 months old. The morphology and hematopoietic composition of KM from WT siblings (n = 6) and Runx1-FLT3/ITD transgenic (n = 6) zebrafish were examined by Giemsa staining (L) and flow cytometry (abbreviation for panel M: M, Myeloid cells; P, Progenitor cells; L, Lymphoid cells; E, Erythroid cells). Data are presented in box plot. **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FLT3: 2322
Runx1: 12394"
Figure legend: "Kidney marrow (KM) was collected from Runx1-FLT3/ITD transgenic zebrafish (F1) at 18 months old. The morphology and hematopoietic composition of KM from WT siblings (n = 6) and Runx1-FLT3/ITD transgenic (n = 6) zebrafish were examined by flow cytometry Data are presented in box plot. The whiskers, boxes, and central lines in panel N represented the minimum-to-maximum values, 25th-to-75th percentile, and the 50th percentile (median), respectively. **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FLT3: 2322
Runx1: 12394"
Figure legend: "O Expression of fst was detected by RT-qPCR in KM from WT sibling and Runx1-FLT3/ITD transgenic zebrafish at 18 months old. The RT-qPCR experiments were performed in triplicates and data were presented as mean ± SEM. **P&lt;0.01."
NCBI gene IDs:
"FLT3: 2322
fst: 100004116
Runx1: 12394"
Figure legend: "P Detection of FST expression, p-ERK1/2, and p-CREB in mononuclear cells from normal peripheral blood stem cell (PBSC) and FLT3/ITD AML patients (diagnostic samples with leukemia blasts &gt; 80%) by Western blotting. ^: non-specific staining of p-ATF1 protein due to the conserved motif."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "B-C The direct binding of p-CREB to human FST promoter was detected by ChIP-PCR (B) and ChIP-qPCR (C). c-Fos was used as positive control of p-CREB target gene. Normal IgG was used as negative control of ChIP. Data information the experiments were performed in triplicates, and the data were presented as mean ± SEM. **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"CREB: 1385
c-Fos: 2353
FST: 10468"
Figure legend: "D Dual luciferase assay demonstrating the direct binding of pCREB on human FST promoter. pRL-CMV, Renilla luciferase vector; pGL-CRE- and pGL-CRE+, firefly luciferase expression driven by human FST promoter with deleted CRE site (CRE-) or wildtype (CRE+); p-GFPSpark, GFP expressing vector; p-CREBY134F, CREBY134F-GFP expressing vector. Data information: the experiments were performed in triplicates, and the data were presented as mean ± SEM. **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"GFP: 
GFPSpark: 
luciferase: 
CRE: 2777477
CREB: 1385
FST: 10468
FST: 100004116"
Figure legend: "E FST expression and FLT3/ITD signaling were detected by Western blotting in Ba/F3-parental (P in short) and Ba/F3-FLT3/ITD (ITD in short) cells."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "F-H Phosphor-flow analysis of p-CREB in Ba/F3-parental, Ba/F3-FLT3/ITD, and Ba/F3-FLT3/ITD cells-treated with FLT3 inhibitor Quizartinib (Qui in short). Isotype antibody were used as control to calculate the mean fluorescence intensity (MFI) ratio (F-G). The transcription and expression of Fst was detected by RT-qPCR after Quizartinib treatment (10 nM) in Ba/F3-FLT3/ITD cells for one day (H). Data information: the experiments were performed in triplicates, and the data were presented as mean ± SEM. **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"FLT3: 2322
Fst: 14313"
Figure legend: "I-K The expression of FST and phosphorylation of CREB were detected by Western Blotting (I and K) and phospho-flow analysis (J) in MOLM-13 (I) and Ba/F3-FLT3/ITD (K) cells treated with Quizartinib and BRD7389 for one day, respectively. Data information: the experiments were performed in triplicates, and the data were presented as mean ± SEM. **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "L RSK and FST expression were detected by Western Blotting after p90RSK knockout by CRISPR/Cas9 in MOLM-13 cells."
NCBI gene IDs:
"CRISPR: 
p90RSK: 6195
Cas9: 901176"
Figure legend: "M The phosphorylation of CREB and FST expression were detected by Western Blotting in Ba/F3-FLT3/ITD cells treated with CREB inhibitor 666-15 for one day. ^: non-specific staining of p-ATF1 protein due to the conserved motif."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "N CREB and FST expression were detected by Western Blotting after CREB knockout by CRISPR/Cas9 in MOLM-13 cells."
NCBI gene IDs:
"CRISPR: 
CREB: 1385
Cas9: 901176"
Figure legend: "O The growth of Ba/F3-parental (with IL-3), Ba/F3-FLT3/ITD (without IL-3) and Ba/F3-FLT3/ITD (with IL-3) cells was measured after three days treatment of CREB inhibitor 666-15 in vitro. Data information: the experiments were performed in triplicates, and the data were presented as mean ± SEM. **P&lt;0.01, ***P&lt;0.001 (Student's t-test)."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "P The rescue effect of CREB inhibitor 666-15 on FLT3/ITD-induced dorsalization and axis duplication in zebrafish embryos at 1 dpf."
NCBI gene IDs:
"FLT3: 2322"
Figure legend: "FST317 and FST344 overexpression resulted in significant increases of FST transcription by RT-qPCR and protein by Western Blot (B) Green, ML-2-GFP; Blue, ML-2-FST317; Red, ML-2-FST344. The RT-qPCR experiments were performed in triplicates (B). Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test). ns: Not significant."
NCBI gene IDs:
"GFP: 
FST: 10468"
Figure legend: "FST317 and FST344 overexpression promoted ML-2 cell growth in vitro (C). Green, ML-2-GFP; Blue, ML-2-FST317; Red, ML-2-FST344. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test). ns: Not significant."
NCBI gene IDs:
"GFP: 
FST: 10468"
Figure legend: "D-E The clonogenicity of ML-2 overexpressing GFP, FST317, and FST344 in vitro for 14 days. The CFU experiments were performed in triplicates (E). Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test). ns: Not significant."
NCBI gene IDs:
"GFP: 
FST: 10468"
Figure legend: "The engraftment of ML-2 (with luciferase gene) overexpressing GFP, FST317, and FST344 was quantified by bioluminescence imaging (F and G) Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test). ns: Not significant."
NCBI gene IDs:
"GFP: 
luciferase: 
FST: 10468"
Figure legend: "The engraftment of ML-2 (with luciferase gene) overexpressing GFP, FST317, and FST344 and the survival of ML-2-engrafted NSG mice in vivo was recorded (H). Survival curve in panel H was analyzed by Log-rank test. *P&lt;0.05, **P&lt;0.01."
NCBI gene IDs:
"GFP: 
luciferase: 
FST: 10468"
Figure legend: "RNA-seq of upregulation of RET, IL2RA and CCL5 after FST344 overexpression in ML-2 cells."
NCBI gene IDs:
"CCL5: 6352
FST: 10468
IL2RA: 3559
RET: 5979"
Figure legend: "RT-qPCR validation of upregulation of RET, IL2RA and CCL5 after FST344 overexpression in ML-2 cells. RT-qPCR experiments were performed in triplicates (J). Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Student's t-test). ns: Not significant."
NCBI gene IDs:
"CCL5: 6352
FST: 10468
IL2RA: 3559
RET: 5979"
Figure legend: "Overall survival analysis of patients from TCGA-AML based on the differential expression of IL2RA"
NCBI gene IDs:
"IL2RA: 3559"
Figure legend: "Overall survival analysis of patients from TCGA-AML based on the differential expression of CCL5."
NCBI gene IDs:
"CCL5: 6352"
Figure legend: "M Activation of MAPK/ERK pathway in ML-2 by FST344 overexpression were validated by Western Blotting. Scale bar = 1 mm."
NCBI gene IDs:
"FST: 10468"
Figure legend: "A FST knockdown in MOLM-13 by shRNA effectively reduced FST expression."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The morphology were measured after FST knockdown in vitro. Scale bar = 10 μm."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The apoptosis (C) measured after FST knockdown in vitro. Scale bar = 10 μm. The apoptosis assays (C) were performed in triplicates. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The clonogenicity of MOLM-13 (D and E) were measured after FST knockdown in vitro. Scale bar = 10 μm. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The engraftment of MOLM-13 after FST knockdown was detected by flow cytometry of human CD45 and mouse CD45.1 positive cells in recipient mouse BM aspiration at week 2 post transplantation."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The engraftment of MOLM-13 after FST knockdown was detected by flow cytometry of human CD45 and mouse CD45.1 positive cells in recipient mouse BM aspiration at week 2 post transplantation. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FST: 100004116"
Figure legend: "The effect of FST knockdown on the survival of NSG mice-engrafted with MOLM-13. scr: scrambled sequence control (7 mice); sh: short hairpin RNA (8 mice for sh1 and sh2 respectively). The survival curve was analyzed by Log-rank test. **P&lt;0.01."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The morphology of MOLM-13 after FST knockout by CRISPR/Cas9 in vitro. Scale bar = 10 μm."
NCBI gene IDs:
"CRISPR: 
FST: 10468
Cas9: 901176"
Figure legend: "The clonogenicity of MOLM-13 after FST knockout by CRISPR/Cas9 in vitro. Scale bar = 10 μm. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"CRISPR: 
FST: 10468
Cas9: 901176"
Figure legend: "The engraftment of MOLM-13 after FST knockout was detected by flow cytometry of human CD45 and mouse CD45.1 positive cells in recipient mouse BM aspiration at week 2 post transplantation."
NCBI gene IDs:
"FST: 10468"
Figure legend: "The engraftment of MOLM-13 after FST knockout was detected by flow cytometry of human CD45 and mouse CD45.1 positive cells in recipient mouse BM aspiration at week 2 post transplantation. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FST: 10468"
Figure legend: "E The effect of FST knockout on the survival of NSG mice-engrafted with MOLM-13 cells. Cas 9: Cas 9 only (10 mice); sgRNA#3: Cas 9 + sgRNA#3 (10 mice); sgRNA#4: Cas 9 + sgRNA#4 (8 mice). Data information: survival curves were analyzed by Log-rank test. *P&lt;0.05."
NCBI gene IDs:
"FST: 10468
Cas 9: 901176"
Figure legend: "F The knockdown efficiency of different FST-specific antisense oligoes (ASOs) in MOLM-13 cells was detected by RT-qPCR after three days treatment in vitro. The knockdown and RT-qPCR experiments were performed in triplicates. Data information: data were presented as mean ± SEM. *P&lt;0.05, **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"FST: 10468"
Figure legend: "Flt3/ITD knock-in mouse were genotyped (A)."
NCBI gene IDs:
"Flt3: 2322"
Figure legend: "The spleen weight (B and C, 4 mice each) were measured in WT siblings and Flt3/ITD-knock-in mice (8 mice each). data were presented in box plot. The whiskers, boxes, and central lines represented the minimum-to-maximum values, 25th-to-75th percentile, and the 50th percentile (median), respectively. **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"Flt3: 2322"
Figure legend: "The serum Fst level (D) were measured in WT siblings and Flt3/ITD-knock-in mice (8 mice each). data were presented in box plot. The whiskers, boxes, and central lines represented the minimum-to-maximum values, 25th-to-75th percentile, and the 50th percentile (median), respectively. **P&lt;0.01 (Student's t-test)."
NCBI gene IDs:
"Flt3: 2322"