EMBO/SourceData huggingface dataset:
Figure legend: "G. Representative IF pictures of WT or UBAP2L KO HeLa cells synchronized in mitosis with DTBR (n=3) to qualitatively assess the localization of PLK1 at the mitotic spindle with α-tubulin staining. ROIs are shown in the corresponding numbered panels. Scale bar, 5µm."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "D. WB analysis of control (siCTL) or siUBAP2L 48h-treated HeLa cells were synchronized with 1mM monastrol for 16h, treated with DMSO or with 10nM of the PLK1 inhibitor BI2536 for 45min, subsequently washed out from monastrol for the indicated time and collected for protein extraction. This moderate treatment is sufficient to restore the aberrant PLK1 catalytic activity observed in UBAP2L KO cells to the levels of the WT, enabling correct mitotic progression but prevents control cells to progress through mitosis. Proteins MW is indicated in kDa. WB is representative of three independent replicates."
NCBI gene IDs:
"UBAP2L: 9898"
Figure legend: "A) Image of 8-9 day old male control (w1118, left) and chmRNAi (right) flies  upon ubiquitous chm  knockdown (left) and the corresponding quantification (right) (N = 6, Paired t-test was performed for statistical significance); B) Image of 8-9 day old male control (w1118, left) and chmRNAi (right) flies  upon fat-body  specific chm  knockdown (left) and the corresponding quantification (right) (N = 4, Paired t-test was performed for statistical significance); C) Image of 8-9 day old male control (w1118, left) and chmRNAi (right) flies  upon neuron  specific chm  knockdown (left) and the corresponding quantification (right) (N = 3, Paired t-test was performed for statistical significance); D) Image of 8-9 day old male control (w1118, left) and chmRNAi (right) flies  upon muscle  specific chm  knockdown (left) and the corresponding quantification (right) (N = 3, Paired t-test was performed for statistical significance) Data information: All replicates are independent biological replicates and errors bars indicate standard error of the mean (SEM). Paired t-test or Tukey test was performed as indicated and non-significant values are not shown (* - p &lt; 0.05, ** - p &lt; 0.01, *** - p &lt; 0.001, **** - p &lt; 0.0001)."
NCBI gene IDs:
"chm: 43928"
Figure legend: "A) Heatmap showing all significant proteins (FDR &lt; 0.05 and Π &lt; 0.05) between control (w1118) and  chmRNAi. Values in the heatmap are scaled raw intensity values. NAs are indicated in grey;"
NCBI gene IDs:
"chm: 43928"
Figure legend: "A) Average  survival curve of control (w1118) and chmRNAi male flies  upon ubiquitous knockdown of chm  with da-Gal4  (N = 4, paired , Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001)); B) Average survival curve of control (w1118) and chmRNAi male flies  upon fatbody  knockdown of chm  with r4-Gal4  (N = 4, paired , Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001)); C) Average survival curve  of control (w1118) and chmRNAi male flies  upon neuronal  knockdown of chm  with elav-Gal4  (N = 4, paired , Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001)); D) Average survival curve of control (w1118) and chmRNAi male flies  upon muscle  knockdown of chm  with mef-Gal4  (N = 4, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001)); E, F Average survival curve  between E) ethanol  (N = 3, paired Log-rank test was performed for each biological replicate. p-values ranged between 0.2 to 0.05 across replicates) and F) RU486  (N = 4, paired, Log-rank test was performed for each biological replicate with all of them having a p-value &lt; 0.05)) and administered control (w1118) and chmRNAi male flies  using Act-GS-Gal4  for adult-specific chm  knockdown; G) Average survival curve  of control (w1118) and chmMYST/+ male flies  (N = 5, paired Log-rank test was performed for each biological replicate with all of them having a p-value &lt; 0.0001). Data information: All replicates are independent biological replicates and errors bars indicate standard error of the mean (SEM). For survival curves, log-rank test was performed for each biological replicate. The displayed p-value is based on all biological replicates."
NCBI gene IDs:
"Act: 31521
chm: 43928
da: 34413
elav: 31000
Gal4: 855828
mef: 36032"
Figure legend: "A) Weight  of 8-9 day old male chmMYST/+ and chmMYST/+;UAS-chmmyc flies  upon weak (left, N = 5, paired) and strong (right, N = 4, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001) chm  overexpression with arm-Gal4 and da-Gal4  respectively (Paired t-test was performed); Data information: All are independent biological replicates and errors bars indicate standard error of the mean (SEM). Paired t-test was performed for the fly weight data and non-significant values are not shown (* - p &lt; 0.05, ** - p &lt; 0.01, *** - p &lt; 0.001, **** - p &lt; 0.0001)."
NCBI gene IDs:
"myc: 
arm: 31151
chm: 43928
da: 34413
Gal4: 855828"
Figure legend: "B) Average survival curve of  chmMYST/+ and chmMYST/+;UAS-chmmyc male flies  upon ubiquitous weak expression of chm   with arm-Gal4  (N = 4, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001); C) Average survival curve  of chmMYST/+ and chmMYST/+;UAS-chmmyc male flies  upon ubiquitous strong expression of chm   with da-Gal4  (N = 3, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001); Data information: All are independent biological replicates and errors bars indicate standard error of the mean (SEM). For  survival curves, log-rank test was performed for each biological replicate. The displayed indicated p-value is based on all biological replicates. "
NCBI gene IDs:
"myc: 
arm: 31151
chm: 43928
da: 34413
Gal4: 855828"
Figure legend: "D) Average survival curve of chmMYST/+ and chmMYST/+;UAS-chmmyc male flies  upon fatbody  expression of chm   with r4-Gal4  (N = 4, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001); E) Average  survival curve  of control  chmMYST/+ and chmMYST/+;UAS-chmmyc  male flies  upon neuronal   expression of chm   with elav-Gal4  (N = 5, paired, Log-rank test was performed for each biological replicate. All replicates had p-values &lt; 0.0001); F) Average survival curve of  chmMYST/+ and  chmMYST/+;UAS-chmmyc male flies  upon muscle  expression of chm   with mef-Gal4  (N = 5, paired, Log-rank test was performed for each biological replicate. p-values ranged between 0.6 to 0.0001 across replicates). Data information: All are independent biological replicates and errors bars indicate standard error of the mean (SEM). For  survival curves, log-rank test was performed for each biological replicate. The displayed indicated p-value is based on all biological replicates. "
NCBI gene IDs:
"myc: 
chm: 43928
elav: 31000
Gal4: 855828
mef: 36032"
Figure legend: "(A qRT-PCR for different ISGs relative to Rps29 using epidermal RNA from 8 week-old (A) K5-R1/R2 and control (CTRL) mice."
NCBI gene IDs:
"Rps29: 20090"
Figure legend: "qRT-PCR for different ISGs relative to Rps29 using epidermal RNA from 5 day- or 9 day-old (B) K5-R1/R2 and control (CTRL) mice."
NCBI gene IDs:
"Rps29: 20090"
Figure legend: "(D) qRT-PCR for different ISGs, Ifnb, and Ifnl3 relative to Rps29 using RNA from freshly isolated, non-cultured keratinocytes of K5-R1/R2 and control (CTRL) mice at P7."
NCBI gene IDs:
"Ifnb: 15977
Ifnl3: 338374
Rps29: 20090"
Figure legend: "(E) qRT-PCR for ISGs relative to Rps29 using RNA from primary keratinocytes of neonatal CTRL and K5-R1/R2 mice."
NCBI gene IDs:
"Rps29: 20090"
Figure legend: "(F) qRT-PCR for ISGs and Cre relative to Rps29 using RNA from primary keratinocytes of neonatal K5-Cre and wild-type (WT) mice."
NCBI gene IDs:
"Cre: 2777477
Rps29: 20090"
Figure legend: "(H) qRT-PCR for RSAD2 and ISG15 relative to RPLP0 using RNA from HaCaT keratinocytes treated for 48 h with the FGFR inhibitor BGJ398 (3.5 μM) or vehicle in the presence of serum."
NCBI gene IDs:
"RPLP0: 
ISG15: 9636
RSAD2: 91543"
Figure legend: "(A) Growth factor-starved primary keratinocytes from WT mice were treated for 3 or 6 h with FGF7 (10 ng/ml) or vehicle (CTRL). RNA samples were analyzed by qRT-PCR for the indicated ISGs relative to Rps29."
NCBI gene IDs:
"Rps29: 20090"
Figure legend: "(B) Serum-starved HaCaT keratinocytes were treated for 6 h with FGF7 or FGF10 (each 10 ng/ml). RNA samples were analyzed by qRT-PCR for IRF7 and RSAD2 relative to RPLP0."
NCBI gene IDs:
"RPLP0: 
IRF7: 3665
RSAD2: 91543"
Figure legend: "(D) RNA samples from untreated, serum-starved primary keratinocytes from WT and Ifnar1-KO mice were analyzed by qRT-PCR for Irf7, Rsad2, Oasl2 and Ifnar1 relative to Rps29."
NCBI gene IDs:
"Ifnar1: 15975
Irf7: 54123
Oasl2: 23962
Rps29: 20090
Rsad2: 58185"
Figure legend: "(E) Growth factor-starved primary keratinocytes from neonatal WT, Ifnar1-KO or Ifnar1/Ifnlr1-KO mice were treated for 24 h with FGF7 (10 ng/ml). RNA samples were analyzed by qRT-PCR for Irf7 relative to Rps29."
NCBI gene IDs:
"Ifnar1: 15975
Ifnlr1: 242700
Irf7: 54123
Rps29: 20090"
Figure legend: "HaCaT keratinocytes were pre-treated for 2 h with the proteasome inhibitors MG132 (10 μm (G) followed by a 20h treatment with FGF7 (10 ng/ml) or vehicle. RNA samples were analyzed by qRT-PCR for IRF7 and IRF1 relative to RPLP0."
NCBI gene IDs:
"RPLP0: 
IRF1: 3659
IRF7: 3665"
Figure legend: "HaCaT keratinocytes were pre-treated for 2 h with epoxomicin (100 nM) (H), followed by a 20h treatment with FGF7 (10 ng/ml) or vehicle. RNA samples were analyzed by qRT-PCR for IRF7 and IRF1 relative to RPLP0."
NCBI gene IDs:
"RPLP0: 
IRF1: 3659
IRF7: 3665"
Figure legend: "Serum-starved HaCaT keratinocytes were treated with FGF7 (10 ng/ml (A and/or IFN-α (500-1000 U/ml) for 12-16 h. RNA was analyzed by qRT-PCR for ISGs relative to RPLP0 (A"
NCBI gene IDs:
"RPLP0: "
Figure legend: "Serum-starved HaCaT keratinocytes were treated with FGF7 different concentrations as indicated (B)) and/or IFN-α (500-1000 U/ml) for 12-16 h. RNA was analyzed by qRT-PCR for ISGs relative to RPLP0"
NCBI gene IDs:
"RPLP0: "
Figure legend: "(D) Human primary keratinocytes were starved and treated for 12 h with FGF7 (10 ng/ml) and/or IFN-α (1000 U/ml). RNA samples were analyzed by qRT-PCR for ISGs relative to RPLP0."
NCBI gene IDs:
"RPLP0: "
Figure legend: "(A) Serum-starved HaCaT cells were treated for 24 h with poly(I:C) (5 μg/ml) in the presence or absence of FGF7 (10 ng/ml). RNA samples were analyzed by qRT-PCR for IFNL1, IFNB, RSAD2, IRF1, IRF7, CGAS, TLR3 or DDX58, relative to RPLP0."
NCBI gene IDs:
"RPLP0: 
CGAS: 115004
DDX58: 23586
IFNB: 3456
IFNL1: 282618
IRF1: 3659
IRF7: 3665
RSAD2: 91543
TLR3: 7098"
Figure legend: "Serum-starved HaCaT cells were infected with HSV-1 (MOI=0.5) in the presence or absence of FGF7 (10 ng/ml) or IFN-α (1000 U/ml). Viral load was determined 24 h post infection (hpi) by qPCR for the HSV-1 Glyc-B gene relative to the human β-actin gene (ACTB) (A)"
NCBI gene IDs:
"ACTB: 
β-actin: 
Glyc-B: 24271469"
Figure legend: "Serum-starved HaCaT cells (D) were infected with HSV-1 (MOI=0.5) in the presence or absence of FGF7 at the indicated concentrations. Virus load was determined by qPCR for HSV-1 Glyc-B relative to ACTB 24 hpi."
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "human primary keratinocytes (HPK, from two donors) (E) were infected with HSV-1 (MOI=0.5) in the presence or absence of FGF7 at the indicated concentrations. Virus load was determined by qPCR for HSV-1 Glyc-B relative to ACTB 24 hpi."
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "(F) Serum-starved HaCaT cells were infected with HSV-1 (MOI=0.5) in the presence or absence of FGF7, FGF10 or FGF2 (10 ng/ml). DNA samples were analyzed by qPCR for Glyc-D DNA relative to ACTB and protein lysates were analyzed by Western blot for Glyc-D and vinculin 10 hpi."
NCBI gene IDs:
"ACTB: 
Glyc-D: "
Figure legend: "(H) Serum-starved HaCaT cells were infected with HSV-1 in the presence or absence of FGF7 (10 ng/ml) or IFN-α (1000 U/ml). Viral load was determined 24 hpi by qPCR for the HSV-1 Glyc-B gene relative to ACTB or by Western blot for the viral protein Glyc-D and GAPDH."
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "(I) Serum-starved HaCaT cells were infected with HSV-1 (MOI = 0.5). Four hours hpi, infected cells were washed and incubated in fresh serum-free medium containing FGF7 (10 ng/ml). Viral load was measured 8 h later by qPCR for Glyc-B relative to ACTB."
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "(J) Serum-starved HaCaT keratinocytes were treated for 6 h with FGF7 (10 ng/ml). RNA samples were analyzed by qRT-PCR for NECT1 (encoding nectin-1) relative to RPLP0."
NCBI gene IDs:
"RPLP0: 
NECT1: 5818
nectin-1: 5818"
Figure legend: "Serum-starved HaCaT cells were infected with HSV-1 (MOI=0.5) +/- FGF7 (10 ng/ml), added at the indicated time points 12 h after infection, RNA samples were analyzed by qRT-PCR for RSAD2, IRF7 and IFNL2 relative to RPLP0 (B),"
NCBI gene IDs:
"RPLP0: 
IFNL2: 282616
IRF7: 3665
RSAD2: 91543"
Figure legend: "Serum-starved HaCaT cells were infected with HSV-1 (MOI=0.5) +/- FGF7 (10 ng/ml), added at the indicated time points 12 h after infection, , DNA samples were analyzed by qPCR for Glyc-B DNA relative to ACTB (C),"
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "Serum-starved HaCaT cells were infected with HSV-1 (MOI=0.5) +/- FGF7 (10 ng/ml), added at the indicated time points D) shows mean Glyc-B DNA and RSAD2, IRF7 and IFNL2 mRNA levels (based on results shown in Fig. 6B and C) in virus-infected cells treated for different periods with FGF7."
NCBI gene IDs:
"IFNL2: 282616
IRF7: 3665
RSAD2: 91543
Glyc-B: 24271469"
Figure legend: "(I, J) HaCaT cells were infected with the ZIKV PF13/251013-18 (MOI = 20) in the presence or absence of FGF7 (20 ng/ml). RNA samples were analyzed by qRT-PCR for OAS1 and MxA relative to RPLP0 72 hpi."
NCBI gene IDs:
"RPLP0: 
MxA: 4599
OAS1: 4938"
Figure legend: "(A) Serum-starved HaCaT cells were infected with HSV-1 (MOI = 0.5) in the presence or absence of FGF7 (10 ng/ml) and the FGFR kinase inhibitors AZD4547 (1 μM) or BGJ398 (3.5 μM). DNA and protein lysates were analyzed by qPCR for Glyc-B relative to ACTB or by Western blot for Glyc-D and GAPDH, respectively, 16 hpi."
NCBI gene IDs:
"ACTB: 
Glyc-B: 24271469"
Figure legend: "HaCaT cells were cultured in DMEM/10% FCS (B) infected with HSV-1 (MOI=0.5) in the presence or absence of AZD45347 (1 μM) and/or BGJ398 (3.5 μM). Viral load was determined by qPCR for Glyc-D relative to ACTB (B)"
NCBI gene IDs:
"ACTB: 
Glyc-D: "
Figure legend: "(D, E) Adult C57BL/6 wild-type (D) or K5-R1/R2 mice and respective controls (E) were infected subcutaneously with HSV-1 (MOI = 10). RNA from infected skin was analyzed by qRT-PCR for Fgf7 relative to Rps29 (D) and DNA was analyzed by qPCR for ICP0, normalized to the host gene Tbx15 48 hpi (E)."
NCBI gene IDs:
"Tbx15: 
Fgf7: 14178
ICP0: 2703389
Rps29: 20090"
Figure legend: "(A) Western blot analysis showing the expression of the WRNIP1 protein in wild-type cells (shWRNIP1WT) and WRNIP1-deficient (shWRNIP1) or mutant (shWRNIP1T294A) cells. MRC5SV fibroblasts were used as a positive control. The membrane was probed with an anti-FLAG or anti-WRNIP1. GAPDH was used as a loading control. Below each lane of the blot the ratio of WRNIP1 protein to total protein, then normalised to MRC5SV, is reported."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(B) Experimental scheme of dual labelling of DNA fibers in shWRNIP1WT, shWRNIP1 and shWRNIP1T294A cells. Cells were pulse-labelled with CldU, and then subjected to a pulse-labelling with IdU.(C) Analysis of replication fork velocity (fork speed) in the cells under unperturbed conditions. The length of the green tracks were measured. Mean values are represented as horizontal black lines (ns, not significant; Student's t-test)."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(E) Experimental scheme of dual labelling of DNA fibers in shWRNIP1WT, shWRNIP1 and shWRNIP1T294A cells. Cells were pulse-labelled with CldU, treated with 4mM HU and then subjected to a pulse-labelling with IdU.(F) Graphs show the percentage of red (CldU) tracts (stalled forks) or red-green (CldU-IdU) contiguous tracts (restarting forks) in the cells. Mean shown, n = 3. Error bars represent standard error. (*, p &lt; 0.05; **, p &lt; 0.01; Student's t test). Representative DNA fiber images are shown. Scale bars, 10 µm."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(G) Experimental scheme of dual labelling of DNA fibers in shWRNIP1WT, shWRNIP1 and shWRNIP1T294A cells. Cells were sequentially pulse-labelled with CldU and IdU as indicated, then treated or not with 4 mM HU.(H) Representative IdU tract length distributions in all cell lines under unperturbed conditions (left graph) or after HU treatment (right graph). Median tract lengths are given in parentheses. See also Tables S1 and S2 for details on the data sets and statistical test. Representative DNA fiber images are shown. Scale bars, 10 µm."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(A) Experimental scheme of dual labelling of DNA fibers in wild-type cells (shWRNIP1WT) or WRNIP1-deficient cells (shWRNIP1). Cells were sequentially pulse-labelled with CldU and IdU as indicated, then left untreated or treated with 4 mM HU in combination or not with 50 µM Mirin.(B) Representative IdU tract length distributions in shWRNIP1WT (left graph) or shWRNIP1 cells (right graph) after treatment. Median tract lengths are given in parentheses. See Tables S1 and S2 for details on the data sets and statistical test. Representative DNA fiber images are shown. Scale bars, 10 µm."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(A) Evaluation of ssDNA accumulation at parental-strand by immunofluorescence microscopy analysis in wild-type (shWRNIP1WT) or WRNIP1-deficient (shWRNIP1) cells. Experimental design of ssDNA assay is shown. Cells were labelled with IdU for 24 h, as indicated, washed and left to recover for 2 h, then treated or not with 4 mM HU. In parallel samples, the MRE11 activity is chemically inhibited with 50 µM Mirin, alone or in combination with HU-induced replication stress. After treatment, cells were fixed and stained with an anti-IdU antibody without denaturing the DNA to specifically detect parental ssDNA. Horizontal black lines represent the mean SE; n = 3. Error bars represent standard error (ns, not significant; **, p&lt;0.01; ****, p&lt;0.0001; two-tailed Student's t test). Representative images are shown. DNA was counterstained with DAPI (blue)."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(B) Analysis of chromatin binding of MRE11 and RAD51 in shWRNIP1WT and shWRNIP1 cells. Chromatin fractions of cells, treated or not with 4 mM HU, were analysed by immunoblotting. The membrane was probed with the anti-WRNIP1, anti-MRE11 and anti-RAD51 antibodies. LAMIN B1 was used as a loading for the chromatin fraction. Total amount of RAD51 and MRE11 (Input) in the cells was determined with the relevant antibodies. LAMIN B1 was used as a loading control. In the graph, the fold increase respect to the wild-type untreated of the normalized ratio of the chromatin-bound RAD51 (or MRE11)/ total RAD51 (or MRE11) is reported for each cell line."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(C) Analysis of DNA-protein interactions between ssDNA and endogenous RAD51 in shWRNIP1WT and shWRNIP1 cells by in situ PLA assay. Experimental designed used for the assay is given. Cells were labelled with IdU for 24 h, as indicated, washed and left to recover for 2 h, then treated or not with 4 mM HU for 4 h. Next, cells were fixed, stained with an anti-IdU antibody without denaturing the DNA to specifically detect parental-strand ssDNA, and subjected to PLA assay as described in the \"Experimental procedures\" section. Antibodies raised against IdU or RAD51 were used to reveal ssDNA or endogenous RAD51 respectively. Each red spot represents a single interaction between ssDNA and RAD51. No spot has been revealed in cells stained with each single antibody (negative control). DNA was counterstained with DAPI (blue). Representative images of the PLA assay are given. Graph shows the number of PLA spots per cell. Horizontal black lines represent the mean value (ns, not significant; **, p &lt; 0.01; two-tailed Student's t test); n = 3."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(D) Localization of WRNIP1, MRE11 and RAD51 to stalled replication forks. Forks were isolated by CldU-co-immunoprecipitation (CldU-IP). shWRNIP1WT or shWRNIP1 cells were pulse-labelled with CldU, then fixed or treated with HU. Cells were cross-linked, and the nuclear extracts were isolated (Input) and subjected to CldU-IP using an anti-CldU antibody (CldU-IP). The membranes were probed with the anti-WRNIP1 or anti-RAD51 antibodies. After stripping, the membranes were probed with an anti-MRE11 antibody. LAMIN B1 and GAPDH were used as loading controls (Input). Ponceau S was used as a loading control of CldU-IP. Dot blot analysis was performed to confirm that equal amounts of immunoprecipitatedDNA from each sample. 10% of each IP was loaded on a nitrocellulose membrane. The membrane was probed with an anti-CldU antibody. The graph shows the normalized ratio of the proteins co-immunoprecipitated with CldU (CldUCo-IP proteins)/ the total of labelled DNAimmunoprecipitated with CldU (CldUIP) for each cell line after replication stress from two independent experiments. The dots in the graph represent the individual data points from each single experiment. Horizontal black line represents the mean value from two replicates; n = 2."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(A) Experimental scheme of pulse-labelling of DNA fibers in wild-type cells (shWRNIP1WT) or WRNIP1-deficient cells (shWRNIP1). Cells were labelled with IdU and exposed or not to 25 μM RAD51 inhibitor, then treated or not with 4 mM HU.(B) Representative IdU tract length distributions in shWRNIP1WT cells (left graph) or shWRNIP1 cells (right graph). Median tract lengths are reported in parentheses. See Tables S1 and S2 for details on the data sets and statistical test. Representative DNA fiber images are reported. Scale bars, 10 µm."
NCBI gene IDs:
"WRNIP1: 56897"
Figure legend: "(C) Scheme of DNA fiber tract analysis in shWRNIP1 cells. Cells were transfected with an empty vector or a plasmid expressing a wild-type human RAD51, and 48 h thereafter labelled with IdU and treated or not with 4 mM HU.(D) Representative IdU tract length distributions in shWRNIP1 cells or shWRNIP1 cells expressing exogenous wild-type RAD51 after HU exposure. Median tract lengths are given in parentheses. See Tables S1 and S2 for details on the data sets and statistical test. Representative DNA fiber images are given. Scale bars, 10 µm. Western blot shows the expression of the RAD51 protein in shWRNIP1 cells. The membrane was probed with an anti-RAD51. LAMIN B1 was used as a loading control."
NCBI gene IDs:
"RAD51: 5892///5889///5890///5888
WRNIP1: 56897"
Figure legend: "(A) Co-immunoprecipitation experiments in HEK293T cells transfected with empty vector or FLAG-WRNIP1 plasmid. Cells were treated or not with HU. After treatment, cell lysates were immunoprecipitated (FLAG-IP) using anti-FLAG antibody. The presence of WRNIP1, BRCA2 and RAD51 was assessed by immunoblotting using the anti-FLAG, anti-RAD51 and anti-BRCA2 antibodies, respectively. Whole cell extracts were analysed (Input). The membrane was probed with the same antibodies used for IP. GAPDH was used as a loading control."
NCBI gene IDs:
"WRNIP1: 56897"