EMBO/SourceData huggingface dataset:
Figure legend: "F-J. Representative images of GFAP immunostaining (F) and quantification (G-J) in controls, Daam2 icKO, Slc4a4 icKO, and double icKO 6 weeks after tamoxifen injection. Scale bar: 20 μm. Data is presented as mean ± SEM. n=10-24 images, N=3-4 mice per genotype. Scale bar: 20 μm. Two-way ANOVA is used for statistics. [*] and [#] indicates comparison with control and double icKO, respectively. **p&lt;0.01, p***&lt;0.001, ##p&lt;0.01."
NCBI gene IDs:
"Daam2: 76441
Slc4a4: 54403"
Figure legend: "RT‐PCR performed on cDNA from RNA isolated from splenic B cells derived from Tam‐treated mb1‐CreERT2 (left lane) or mb1‐CreERT2;Sykfl/flmice (right lane); the Syk‐ and Hprt‐specific amplification products were identified by agarose gel electrophoresis with Hprt used as an endogenous loading control."
NCBI gene IDs:
"Cre: 
Hprt: 
ER: 13983///13982
Syk: 20963"
Figure legend: "Immunoblot analysis of proteins isolated from B cells derived from spleens of mb1‐CreERT2 (left lane) or mb1‐CreERT2;Sykfl/fl (right lane) mice both treated with Tam as described; blots were probed with anti‐Syk and anti‐GAPDH Ab, GAPDH being used as a loading control."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "Intracellular flow cytometric analysis for Syk expression in B cells derived from the LN of Syk‐deficient and control mice."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "Genomic DNA analysis of Tam‐treated mb1‐CreERT2 or mb1‐CreERT2;Sykfl/flmice (as indicated). Upper row: amplification of floxed (fl) and wt (+) alleles. Middle row: amplification of the deleted (d) allele. Lower row: loading control (TC21, a gene unaffected by the deletion of Syk). Established control DNA was used to visualize the individual bands (as indicated)."
NCBI gene IDs:
"TC21: 
Syk: 20963"
Figure legend: "A, B Flow cytometric analysis of B cells from (A) the BM and (B) the SP of mb1‐CreERT2 control (left) and mb1‐CreERT2;Sykfl/flmice (right) treated with Tam as described in the Materials and Methods section. The BM cells were stained with anti‐IgM and anti‐B220, and the SP cells with anti‐CD19 and anti‐CD93 or anti‐CD23 and anti‐CD21. The gated regions in the dot blots correspond to individual B‐cell populations: (A) Bone marrow: gate P (B220+IgM−) pro‐/pre‐B cells, gate I (B220loIgM+) immature B cells, gate M (B220hiIgM+) mature B cells; (B) spleen: gate T (CD19+ CD93+) transitional B cells (T), gate M (CD19+ CD93−) mature B cells; in CD93−splenic B cells, gate M (CD23hiCD21int CD93−) mature follicular B cells and gate MZ (CD23lo CD21hi CD93−) marginal zone B cells. The numbers in the dot plots indicate the mean relative frequency of cells in the gate.C-E Statistical analysis of absolute cell numbers per Tam‐treated mouse: filled circles indicating cells obtained from mb1‐CreERT2 control mice and open circles from mb1‐CreERT2;Sykfl/flmice. (C) Statistical analysis of the absolute cell numbers of pro‐/pre‐, immature and mature recirculating B cells in the BM. (D) Absolute cell numbers of transitional, M and MZ B cells found in the SP. (E) Statistical analysis of the absolute cells numbers of peritoneal B cell subsets B1‐a and B1‐b. An asterisk (*) marks statistically significant differences (P 0.05), two asterisks (**) indicate P 0.01, P‐values were obtained using two‐tailed Student's t‐test. Cell numbers of four to five mice per group are shown, with each dot representing an individual animal."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "A Flow cytometric analysis of IgM, CD19 and CD23 expression of splenic B cells from Tam‐treated mb1‐CreERT2 control (dashed line) and mb1‐CreERT2;Sykfl/flmice (solid line)."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "B Flow cytometric analysis of tyrosine phosphorylation (pY) in splenic B cells from Tam‐treated mb1‐CreERT2 and mb1‐CreERT2;Sykfl/flmice stimulated with medium (dotted line) or 50 μM pervanadate (dashed and solid lines)."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "C, D Flow cytometric analysis of the intracellular Ca2+ influx in purified splenic B cells derived from Tam‐treated mice. mb1‐CreERT2 or mb1‐CreERT2;Sykfl/fl were treated with (C) 10 μg/ml anti‐Kappa F(ab′)2 fragments or (D) 1 μM ionomycin."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "Ex vivo activation assay of splenic mature B cells from Tam‐treated mb1‐CreERT2 (upper row) or Tam‐treated mb1‐CreERT2;Sykfl/flmice (lower row). Purified splenic B cells were either left unstimulated (medium) or were stimulated with the indicated stimuli. The cells were analyzed by flow cytometry after 24 h. Shown are CD19 versus CD86 dot plots. 7‐AAD was included to distinguish dead from viable cells. The numbers in the quadrants indicate the relative frequencies of cells in the gate."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "Ex vivo proliferation assay with mature B cells from Tam‐treated mb1‐CreERT2 (upper row) or Tam‐treated mb1‐CreERT2;Sykfl/flmice (lower row). The cells were incubated with the indicated stimuli for 90 h."
NCBI gene IDs:
"Syk: 20963"
Figure legend: "A Flow cytometric analysis of splenic B cells from Rag2−/−;γc−/− mice. CD19 versus CD3ε dot plots are shown. The mice were injected i.v. with 5 × 106 splenic (CD19+ CD93−) B cells and (CD3ε+) T cells from Tam‐untreated mb1‐CreERT2 control or mb1‐CreERT2;Sykfl/fl mice. The recipient mice were treated with Tam beginning 1 day after transfer as described in the Materials and Methods section.B Quantitative analysis of B and T cells from Rag2−/−;γc−/− mice repopulated with mb1‐CreERT2 or mb1‐CreERT2;Sykfl/fl splenocytes. Each symbol represents an individual mouse. Filled circles and squares represent Syk+/+ B and T cells, respectively; open circles and squares represent Sykfl/fl B and T cells."
NCBI gene IDs:
"Rag2: 
Syk: 20963"
Figure legend: "(E) Representative western blot  of scr or miR-30c transfected  TREx  cells analysed for  ORF65 expression with  GAPDH as a loading control. Densitometry analysis performed on n=3. Data information: data are presented as mean ± SD. **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"miR-30c: 407031"
Figure legend: "(F) Representative  western blot  of scr or  miR-29b transfected TREx cells analysed for ORF65 expression  with GAPDH as a loading control. Densitometry  analysis performed on n=3. Data information: data are presented as mean ± SD. **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"miR-29b: 407024"
Figure legend: "(C) Representative  western blot of  ORF65 levels in scr and circHIPK3 KD cell lines with  GAPDH as a loading control. Densitometry  analysis of n=3 western blots. Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"HIPK3: 10114"
Figure legend: "(D) Representative  western blot of  DLL4 levels in scr and circHIPK3 stable TREx cells at 0 and 24 hours post induction with GAPDH as a loading control.  Densitometry analysis of n=3. Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"HIPK3: 10114"
Figure legend: "(F) Representative western blot  of DLL4 levels in scr or  miR-30c transfected  TREx  cells at 0, 24 and 48 hours post induction with  GAPDH as a loading control. Densitometry  analysis of n=3 Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"miR-30c: 407031"
Figure legend: "(J) Representative western blot of DLL4 levels in scr or  miR-30c antagomiR transfected  TREx  cells at 0, 24 and 48 hours post induction with  GAPDH as a loading control. Densitometry analysis of n=3. Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"miR-30c: 407031"
Figure legend: "(B) Representative western blot  of DLL4 levels in scr and  DLL4 KD TREx  cells with GAPDH as a loading control,  densitometry analysis is n=3. Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"DLL4: 54567"
Figure legend: "(C) Representative western blot  of ORF65 levels at 0, 24 and 48 hours post induction in scr and DLL4 KD TREx  cells, GAPDH was used as a loading control and densitometry analysis performed on n=3. Data information: data are presented as mean ± SD. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 (Unpaired Student's t-test). All repeats are biological."
NCBI gene IDs:
"DLL4: 54567"
Figure legend: " Growth was tested in the presence of ceftriaxone at 8 µg/ml (+ ceftriaxone) or in the absence of the drug (- ceftriaxone) on BHI agar plates supplemented with 40 µM IPTG and 1% L-arabinose for induction of ycbB and relA', respectively. (A) BW25113 ΔrelA derivatives harboring plasmids pKT2(ycbB), pKT8(relA') and the vectors pHV6 and pHV7 used to construct these plasmids, respectively. Expression of β-lactam resistance requires induction of both ycbB and relA'."
NCBI gene IDs:
"ycbB: 945541
relA: 947244"
Figure legend: "  (B) BW25113(ycbB, relA') and its derivatives obtained by individual deletion of endopeptidase genes. BW25113(ycbB, relA') is an abbreviated name for BW25113 ΔrelA pKT2(ycbB) pKT8(relA').  "
NCBI gene IDs:
"ycbB: 945541
relA: 947244"
Figure legend: " Growth of BW25113(ycbB, relA'), BW25113(ycbB, relA') ΔmepM, and its derivatives harboring plasmids encoding MepM, MepM H393A, and MepM ΔLytM were tested in the presence of ceftriaxone at 8 µg/ml (+ ceftriaxone) or in the absence of the drug (- ceftriaxone) in BHI agar plates supplemented with 40 µM IPTG and 1% L-arabinose for induction of ycbB and relA', respectively. The genes encoding MepM, MepM H393A, and MepM ΔLytM were inserted into the vector pHV9 under the control of the PphlF promoter, which is inducible by 2,4-diacetylphloroglucinol (DAPG). Basal level of expression of mepM under the control of PphlF was sufficient to restore ceftriaxone resistance in the absence of the inducer."
NCBI gene IDs:
"phlF: 
ycbB: 945541
mepM: 946376
MepM: 946376
relA: 947244"
Figure legend: " The mepM gene was expressed under the control of TIS1 or TIS2 (translation initiation) and of PrhaBAD (the L-rhamnose-inducible promoter of vector pHV30). Growth of BW25113(ycbB, relA') ΔmepM harboring the pHV30 derivatives was tested on BHI agar supplemented with ceftriaxone 8 µg/ml, IPTG 40 µM (induction of ycbB) and L-arabinose 1% (induction of relA'). (A) For TIS2, growth around paper disks containing 1 or 2 mg of L-rhamnose indicated that induction of the expression of mepM was required for ceftriaxone resistance. In contrast, a higher level of translation from TIS1 was sufficient for ceftriaxone resistance in the absence of the inducer. (B) The experiment was repeated with bacteria pre-exposed to L-rhamnose that were harvested at the vicinity of the disk containing 2 mg of L-rhamnose. Expression of ceftriaxone resistance remained dependent on the presence of L-rhamnose indicating that the requirement for MepM is not transient. The diameter of the growth zones is larger in panel (B) than in panel (A) as expected from the fact that bacteria in the inoculum used in (B) had been grown in the presence of the inducer and already contained MepM. In (A) growth is only possible after diffusion of L-rhamnose in the medium prior to the action of ceftriaxone. At a distance from the disk, diffusion was not sufficiently rapid to observe resistance. "
NCBI gene IDs:
"ycbB: 945541
MepM: 946376
mepM: 946376
relA: 947244
rhaBAD: 948401///948400///948399"
Figure legend: " (A) rpHPLC chromatograms of sacculi isolated from BW25113 grown in minimal medium to stationary phase and digested by lysozyme (upper panel) or by lysozyme and 5 µM MepM (lower panel). Absorbance was monitored at 205 nm (mAU, milli-absorbance unit). "
NCBI gene IDs:
"MepM: 946376"
Figure legend: " Functional complementation of the mepM deletion in BW25113(ycbB, relA') ΔmepM was performed with the pHV30 vector or recombinant plasmids encoding each of the eight endopeptidases under the control of the PrhaBAD promoter and of the TIS1 translation initiation signal. Induction of endopeptidase (ED) genes was performed with 0.2% L-rhamnose in the presence or absence of 8 µg/ml ceftriaxone. BHI agar plates contained 40 µM IPTG and 1% L-arabinose for induction of ycbB and relA', respectively. "
NCBI gene IDs:
"ycbB: 945541
mepM: 946376
relA: 947244
rhaBAD: 948401///948400///948399"
Figure legend: " (B) The plating efficiency assay was performed with derivatives of BW25113 Δ8EDs pHV53(mepM) harboring the vector pHV7 or recombinant plasmids carrying each of the eight endopeptidase genes under the control of the ParaBAD promoter. In this assay, functional replacement of MepM is detected based on growth in media containing 0.2% or 1% L-arabinose for expression of the endopeptidase gene carried by vector pHV7, while by-passing the requirement for induction of the mepM copy of pHV53 by L-rhamnose. Complementation was observed with both concentrations of inducer for mepH, mepS, and pbpG. Overproduction of mepM encoded by the pHV7 derivative in the presence of the high dose of L-arabinose (1%) was lethal. The right panel presents the growth control performed in the presence of 0.2% L-rhamnose for induction of the mepM copy carried by pHV53. "
NCBI gene IDs:
"araBAD: 
mepH: 945210
mepM: 946376
MepM: 946376
mepS: 946686
pbpG: 946662"
Figure legend: " (B) Functional complementation of the mepK deletion of BW25113 ΔmepK pHV63(ycbB) was performed with the pHV30 vector or recombinant plasmids encoding each of the eight endopeptidases under the control of the PrhaBAD promoter. Induction of ycbB and of endopeptidase (ED) genes was performed with 0.2% L-arabinose and 1% L-rhamnose, respectively. BHI agar plates contained chloramphenicol (20 µg/ml) to counter-select loss of pHV63(ycbB). "
NCBI gene IDs:
"ycbB: 945541
mepK: 945538
rhaBAD: 948401///948400///948399"
Figure legend: " (A) Functional complementation of the mepK deletion of BW25113 ΔmepK pHV63(ycbB) was performed with the pHV30 vector or recombinant plasmids encoding mepA, mepK, mepS or derivatives encoding catalytically inactive endopeptidases under the control of the PrhaBAD promoter. (B) The complementation assay was repeated for BW25113 Δ7EDs pHV63(ycbB), which was obtained by deletion of all chromosomal endopeptidase genes except mepM. Induction of ycbB and of endopeptidase (ED) genes was performed with 0.2% L-arabinose and 1% L-rhamnose, respectively. BHI agar plates contained chloramphenicol (20 µg/ml) to counter-select loss of pHV63(ycbB). "
NCBI gene IDs:
"ycbB: 945541
mepA: 946812
mepK: 945538
mepM: 946376
mepS: 946686
rhaBAD: 948401///948400///948399"
Figure legend: " (A) Growth curves of (i) BW25113(ycbB, relA') and its ΔsltY derivative; and (ii) strain M1 and its derivatives (M1.1 to 1.4) harboring mutations in sltY. The growth medium contained (i) 40 µM IPTG, 1% L-arabinose, and 8 µg/ml ceftriaxone for BW25113(ycbB, relA') and its ΔsltY derivative or (ii) 50 µM IPTG and 16 µg/ml ampicillin for M1 and its derivatives. "
NCBI gene IDs:
"ycbB: 945541
relA: 947244
sltY: 948908"
Figure legend: " C, Dose-response curves of BEAS-2B and BEAS-2B-KRAS cells treated for 72 hours with inhibitors of FGFR (AZD4547, BGJ398) and PLK1 (BI2536, BI6727), alone or combination. Values of Fa and CI are shown underneath, with CI&lt;1.0 indicating synergistic effect. Data were from three independent experiments (n=3), error bar: SD "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " D, BEAS-2B and BEAS-2B-KRAS treated with the indicated drugs, alone or combination for 72 hours, were cultured in drug-free medium for additional 7-21 days. Surviving cells after the treatment were fixed and visualized by crystal violet staining. "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " C, Clongenic assay of KRAS-mutant lung and pancreatic cells (MIA PaCa-2, H2122, H2009, H23, H358 and A549) treated with control (DMSO), AZD4547 (5 µM) and BI2536 (5 nM), alone or combination. "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " D, Apoptotic assay of KRAS-mutant cancer cells (H358, H441, A549, and SW620) and KRAS-WT lung cancer cells (EBC-1) treated with vehicle (DMSO), AZD4547 (5 µM) and BI2536 (5 nM), alone or in combination for 48h. Data are presented as mean ± SD (n=3). ***P&lt;0.001 and ****P&lt;0.0001 by two-way ANOVA with Tukey's multiple comparisons test. "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " E, KRAS mutant lung (A549, H358，H441), pancreatic (MIAPaCa-2) and colon (SW620) cancer cells were transfected with control siRNAs, FGFR1- and PLK1-specific siRNAs, alone or combination. Cell viability was determined 72 h post transfection. Data are presented as mean ± SD (n=3). **p&lt;0.01, ****p&lt;0.0001, and ns P＞0.05 by two-way ANOVA with Tukey's multiple comparisons test. ns, no significance. "
NCBI gene IDs:
"FGFR1: 2260
KRAS: 3845
PLK1: 5347"
Figure legend: " D, E, Immunoblots (D) and viability assay (E) of H358 and A549 cells transfected with E2F1-specific or control siRNAs followed by treatment (48 h post transfection) with vehicle (DMSO) or combined AZD 4547 (5 µM)/BI2536(5 nM) for additional 24 hours. Data were shown as mean ± SD (n=3). **P&lt;0.01, ****P&lt;0.0001 and P＞0.05 (ns) by two-way ANOVA with Tukey's multiple comparisons test. "
NCBI gene IDs:
"E2F1: 1869"
Figure legend: " F, H358 cells transfected with E2F1-specific or control siRNAs for 24h were subsequently treated with vehicle (DMSO), AZD4547 (5 µM) and BI2536 (5 nM), alone or combination for 48h before apoptotic assay. Data were shown as mean ± SD (n=3). *P&lt;0.05, **P&lt;0.01 and P＞0.05 (ns) by two-way ANOVA with Tukey's multiple comparisons test. "
NCBI gene IDs:
"E2F1: 1869"
Figure legend: " F, Immunoblots of H358 cells transfected with ATG5-specific or control siRNAs for 48h and before treated for 24h with vehicle (DMSO) or combined AZD 4547 (5 µM)/BI2536 (5 nM). "
NCBI gene IDs:
"ATG5: 9474"
Figure legend: " A, Growth curve of a KRAS-mutant lung cancer PDX (BE564T) treated with vehicle, HCQ (30 mg/kg/day), AZD4547 (10 mg/kg/day) and BI6727 (5 mg/kg/day), alone or in combination. *P&lt;0.05, ***P&lt;0.001 and P＞0.05 (ns) by one-way ANOVA with Tukey's multiple comparisons test. Data are the mean of tumor volume of each group (5 mice/group); error bar: SD "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " F-K, Growth curves (F, I) of KRAS-mutant lung cancer PDXs (PF139, PF563) treated with vehicle, HCQ (30 mg/kg/day), AZD4547 (10 mg/kg/day) and BI6727 (5 mg/kg/day), alone or in combination. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 by one-way ANOVA with Tukey's multiple comparisons test. Data are the mean of tumor volume of each group (4 mice/group); error bar: SD Relative tumor volume (G, J) and weights (H, K) of the PDX tumors after the treatment. *P&lt;0.05, **P&lt;0.01, ***P&lt;0.001 and P＞0.05 (ns) by unpaired two-tailed t-test. Data are the mean ± SD (error bar) of tumor weights of each group (4 mice/group). "
NCBI gene IDs:
"KRAS: 3845"
Figure legend: " F, Box plots showing the percentage of Ki67-positive cells in different treatment groups. Data are based on IHC staining of lung tissue section of LSL-KrasG12D mice. *P&lt;0.05 and P＞0.05 (ns) by two-way ANOVA with Tukey's multiple comparisons test. The boxplots span from the first to third quartile, the whiskers extend to 1.5× the interquartile range (IQR). The horzontal line within the boxes represents the median of each group of 10 replicates (n=10). "
NCBI gene IDs:
"Kras: 16653"
Figure legend: "C-F (C, E) Immunofluorescence labelling with an antibody that recognizes both full-length and cleaved Xkr8 in developing (P0, P8) and adult S1 cortex and hippocampus. (D, F) Immunofluorescence labelling with an antibody recognizing full-length uncleaved Xkr8 in developing (P0, P8) and adult S1 cortex and hippocampus. Loss of Xkr8 immunosignal in double transgenic Xkr8flx/flx;Emx1::Cre animals (Xkr8 cKO) is observed with both Xkr8 antibodies (C-F)."
NCBI gene IDs:
"Cre: 2777477
Emx1: 13796
Xkr8: 381560"
Figure legend: "A, B Organotypic slices of Xkr8 WT and cKO hippocampus with Thy1::GFP neurons (green) were labelled with PtdSer-binding fluorescently tagged Annexin V (magenta) at 16-19 DIV. The fluorescence of bound Annexin V was quantified on ImageJ by measuring the integrated density of fluorescent structures within the same volume for each slice and expressing the value as fluorescence units (f.u.; two-tailed Student's t-test, each dot represents an individual slice preparation, n = 4)."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "C Immunofluorescence labelling of active caspase-3 in developing brain from P8 to P28 in Xkr8 WT and Xkr8 cKO brains was quantified and compared by nested design and mixed model ANOVA (F(2,30) = 0.86, p = 0.433), each dot represents an individual mouse (n = 6 per age group)"
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "A-C The size and density of axonal boutons on Thy1::GFP axons (green) in Xkr8 WT and cKO animals (nested design and mixed model ANOVA, each dot represents an individual mouse, n = 6 per age and genotype group)."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "D, E Dendritic spine morphology (D) and density (E) of Thy1::GFP+ CA1 neurons in Xkr8 WT and cKO animals from P8 to P28 (nested design and mixed model ANOVA, each dot represents an individual mouse, n = 5-6 per age and genotype group)."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "J-L The density of cortical (vGluT1+, J) and thalamic (vGluT2+, K) and the ratio of vGluT1+ and vGluT2+ inputs (L) in Xkr8 WT and cKO brains (nested design and mixed model ANOVA, each dot represents an individual mouse, n = 6 per age and genotype group)."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "A-F The immunolabelling of internalized pan-axonal material (A, B), pre-synaptic vGluT1 particles (C, D) and post-synaptic PSD95 particles (E, F) within Iba1+ microglia in P8 cortex of Xkr8 WT and Xkr8 cKO mice was quantified per individual 3D reconstructed microglial cell. The total volume of internalized particles was normalized to microglial cell volume and compared by nested design and mixed model ANOVA, each dot represents an individual mouse (n = 3 per genotype group). Data presented as mean ± SEM; ** p &lt; 0.01, **** p &lt; 0.0001; scale bar 10 µm, grid cell 10 µm."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "A, B Corticospinal axons of the axonal bundles of pyramidal tracts in medulla of P8 and P28 Xkr8 WT and cKO mice were visualized by Palmgren staining and quantified per each bundle, delineated by a dashed line (Mann-Whitney test, n = 6 mice per genotype, the data are presented as median and quartiles)."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "To label callosal projections in vivo, cholera toxin subunit B (CTB) was injected stereotactically into corpus callosum of Xkr8 WT and cKO brains at P30-32. Back-labelled neurons were quantified in L4 of primary (S1) and secondary (S2) somatosensory cortex."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "Xkr8 cKO neurons had increased mean sEPSC amplitude Data were analyzed by two-tailed Student's t-test ; n = 7-14 neurons per genotype."
NCBI gene IDs:
"Xkr8: 381560"
Figure legend: "F-H Evoked excitatory postsynaptic currents (eEPSC) of Xkr8 cKO neurons showed a trend to decrease (p = 0.08) (F, G) and the ratio of eEPSC and sEPSC amplitudes was lower in Xkr8 knockout neurons (H). two-way repeated measures ANOVA (H); n = 7-14 neurons per genotype."
NCBI gene IDs:
"Xkr8: 381560"