EMBO/SourceData huggingface dataset:
Figure legend: "(E) Immunostaining of Galectin-3, CathD and CD68 in spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Scale bar = 10 µm."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "TMEM106B and PGRN deletion results in increased autofluorescence in the spinal cord of 5-month-old mice. n=3. Scale bar = 10 µm."
NCBI gene IDs:
"PGRN: 14824
TMEM106B: 71900"
Figure legend: "TMEM106B and PGRN deletion results in increased autofluorescence in the thalamus of 5-month-old mice. n=3. Scale bar = 10 µm."
NCBI gene IDs:
"PGRN: 14824
TMEM106B: 71900"
Figure legend: "(G, H) Western blot analysis of lysosomal proteins in the spinal cord lysates from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Asterisk indicates non-specific bands. n=3."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(A) Spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/- and Tmem106b-/-Grn-/- mice were stained with anti-neurofascin, anti-LAMP1 and anti-CathD antibodies. Representative images from three independent mice were shown. Scale bar = 10 µm. (B) Quantification of LAMP1 positive vacuoles (&gt; 2 µm) for experiments in (A). 4-6 sections/spinal cord from 3 mice were analyzed for each genotype. Data presented as mean ± SEM. Unpaired Student's t test: ****, p&lt;0.0001. "
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(A Immunostaining of GFAP with LAMP1 and Cath D in the spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Scale bar = 5 µm."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "B) Immunostaining of IBA1 with LAMP1 and Cath D in the spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Scale bar = 5 µm."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(A) Accumulation of autophagosomes and lysosomes in the spinal cord sections from a 5-month-old Tmem106b-/-Grn-/- mouse as shown by transmission electron microscope analysis. Scale bar = 1µm. Arrows: autophagosome; arrow heads: lysosomes; (a): axon."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(B) Accumulation of myelin debris and amorphous inclusions in the microglia in the spinal cord sections from a 5-month-old Tmem106b-/-Grn-/- mouse as shown by transmission electron microscope analysis. Scale bar = 1µm. Arrows: myelin debris; arrow heads: dark amorphous inclusions; (m): microglia."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(C) Western blot analysis of LC3-I and LC3-II in the spinal cord lysates from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. n=3. Data presented as mean ± SEM. One-way ANOVA tests with Bonferroni's multiple comparisons: ****, p&lt;0.0001."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(D Immunostaining of TFE3 and IBA1 in the spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "E) Immunostaining of TFE3 and IBA1 in the spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Nuclear signals of TFE3 in IBA1 positive microglia were quantified. n=3. Data presented as mean ± SEM. One-way ANOVA tests with Bonferroni's multiple comparisons: ***, p&lt;0.001, ****, p&lt;0.0001. Scale bar = 10 µm."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(A, B) Western blot analysis of p62, ubiquitin (Ub), TDP-43 and p-TDP-43 in RIPA- and urea-soluble fractions from spinal cord (C5-C8) and brain of 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. Spinal cord: n=3; brain: n=4. Data presented as mean ± SEM. One-way ANOVA tests with Bonferroni's multiple comparisons: *, p&lt;0.05; **, p&lt;0.01***, p&lt;0.001, ****, p&lt;0.0001."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "(C, D) Immunostaining of p62 and ubiquitin in the spinal cord sections from 5-month-old WT, Tmem106b-/-, Grn-/-, and Tmem106b-/-Grn-/- mice. The number of p62 or Ub positive puncta was quantified. n=3. Data presented as mean ± SEM. One-way ANOVA tests with Bonferroni's multiple comparisons: ***, p&lt;0.001, ****, p&lt;0.0001. Scale bar = 10 µm."
NCBI gene IDs:
"Grn: 14824
Tmem106b: 71900"
Figure legend: "a, Images show the localisation of endogeneous  SEPT2 and SEPT7 in RPE1 ARLB13B-mRuby2  cells with basal body staining. The insets to the right show enlargements of the cilium. Scale bar: 4µm (large) and 2µm (small). b, STED microscopy images showing ciliated RPE1  cells expressing EGFP-SEPT2 or SEPT7-EGFP and stained for ARL13B. Scale bar: 1µm."
NCBI gene IDs:
"mRuby2: 
ARLB13B: 200894"
Figure legend: "g, Electron microscopy of RPE1 WT and SEPT2-KO  cilia. Black arrows point to the  axoneme and basal body  (BB). Scale bar: 400nm. h, Quantification of (g). Only cilia  detected from bottom (BB) to  tip in serial sections were quantified. N&gt;15 cilia from two independent experiments. Data include mean ±s.d.; P values are calculated by unpaired Wilcoxon-Mann-Whitney Rank Sum Test"
NCBI gene IDs:
"SEPT2: 4735"
Figure legend: "i-j, RPE1 WT and SEPT2-KO cells after 48h SS were treated with solvent control (DMSO), cytochalasin D (CytoD) and/or nocodazole  (Noco) for 3h before  immunostaining  with the indicated antibodies. Representative images (i) and quantifications of  PS  (Glu tub) and whole cilia (ARL13B) length (j) are shown. The numbers above the cartoons in (j) show the ratio of the calculated DS/whole cilia length. Scale bar: 3µm. Three biological replicates, n=100  cilia per sample and repetition. Data show include mean ±s.d.; P values are calculated by two-tailed unpaired student t-test"
NCBI gene IDs:
"SEPT2: 4735"
Figure legend: "e-f, RPE1 WT and SEPT2-KO cells were treated with control or KIF7  siRNA after 48h SS  and  immunostained with the indicated antibodies. Representative images (e) and quantifications of proximal segment  (Glu tub) and whole  cilia (ARL13B) length (f) are shown. The numbers above the cartoons in (f) show the ratio of the calculated  distal segments /whole  cilia length. Three biological replicates, n=100  cilia per sample and repetition. Scale bar: 2µm. Data include mean ±s.d. and P values are calculated by two-tailed unpaired student t-test."
NCBI gene IDs:
"KIF7: 374654
SEPT2: 4735"
Figure legend: "d, FRAP experiment of SSTR3-GFP in RPE1 WT and SEPT2 KO cells. Cells were starved for 48h before the beginning of inspection and imaged every 30sec for 10mins after bleaching. Scale bar: 5µm. e, Percentage of normalised  SSTR3-GFP signal recovery at whole cilia  after photobleching from (d). n=20  cilia are quantified from three biological replicates Data include mean ±s.d. and P values are calculated by two-tailed unpaired student t-test."
NCBI gene IDs:
"SEPT2: 4735"
Figure legend: "f-g, Influence of MKS3  depletion on  cilia length of  RPE WT and SEPT2-KO cells. Cells are treated with the indicated siRNA and  serum starved (SS) for 48h before  immunostaining  with the indicated antibodies. Representative images (f) and quantifications of proximal segment  (Glu tub) and whole cilia (ARL13B) length (g) are shown. The numbers above the cartoons in (g) show the ratio of the calculated distal segments/whole cilia  length. Scale bar: 2µm. Three 3 biological replicates, n=100 cilia per sample and repetition. Data include mean ±s.d. and P values are calculated by two-tailed unpaired student t-test."
NCBI gene IDs:
"SEPT2: 4735
MKS3: 91147"
Figure legend: "h-i, A functional TZ  is required for  KIF7 cilia tip localisation in RPE1  cells. KIF7  signal intensities are measured in ciliated RPE1 cells treated with control or MKS3 siRNA and 48h SS. Representative images (h) and quantification of  KIF7  signal intensity at the  base and tip of cilia  (h) are shown. Scale bar: 2µm. Three biological replicates, at least 60  cilia per sample and repetition. Data include mean ±s.d. and P values are calculated by two-tailed unpaired student t-test."
NCBI gene IDs:
"MKS3: 91147"
Figure legend: "a,  Time-lapse images of RPE1 WT and SEPT2-KO cells stably expressing ARL13B-GFP and γ-tubulin-mRuby2. Cells are starved for 32h before the beginning of inspection and imaged every 15min for 9.5h. Scale bar: 5µm. b, Quantification of (a) showing changes in  cilia  length during  time-lapse imaging. Three biological replicates, 10 cilia  per sample and repetition. c, Time-lapse images show ectocytosis  events observed in RPE1 SEPT2-KO cells. Experiments are done as in (a) with exception that cells are imaged every 15min. Scale bar: 2.5µm."
NCBI gene IDs:
"SEPT2: 4735"
Figure legend: "g-h, RPE1 SEPT2-KO cells expressing  ARL13B-mRuby2 and Neongreen-EFHC1 after 48h SS and immunostained  with Glu-tub antibody. Representative images (g) and quantifications of  proximal segment (Glu tub), EFHC1  and whole cilia (ARL13B) length (h) are shown. Three biological replicates, n=100  cilia per sample and repetition. Scale bar: 2µm."
NCBI gene IDs:
"SEPT2: 4735"
Figure legend: "Gel shift assay of biotin-labeled mascRNA and 5S rRNA with the cytosol (Cyto) and the postnuclear membrane lysate (Mem)."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "Northern blotting of mascRNA (top panel) and western blotting of β-Tubulin and Mortalin (bottom two panels) in the cytosolic fractions and the postnuclear membrane fractions of HEK293 and HepG2 cells."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "SDS-PAGE analysis and coomassie staining of the proteins in the RNA pulldown samples. Biotin-labeled mascRNA (S) and the antisense RNA (AS) were used to pull down the RNA-protein complexes.   Representive data of mass spectrometry analysis of the RNA pulldown samples. Proteins most enriched in mascRNA (S) RNA pulldown sample are listed.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Western blots of the proteins in mascRNA S and AS pulldown samples (top 4 panels), and mascRNA and menRNA pulldown samples (Bottom 2 panels). β-tubulin was used as a loading control."
NCBI gene IDs:
"menRNA: 
mascRNA: 109136578"
Figure legend: "RT-PCR analysis of RNAs extracted from ARS immunoprecipitation (RIP) samples (erro bars: mean ± SD; biological replicates n = 3). Eight complex-forming ARSs (DARS, EPRS, IARS, KARS, LARS, MARS, QARS and RARS) and GARS tagged with FLAG were overexpressed in HEK cells. ARS-RNA complexes were imminoprecipitated with anti-FLAG Magnetic Beads. The cell line constructed with PQCXIP vector (PQ) was used as a negative control. GAPDH was used as an internal control for qRT-PCR."
NCBI gene IDs:
"GAPDH: 
FLAG: 
DARS: 1615
EPRS: 2058
GARS: 2617
IARS: 3376
KARS: 3735
LARS: 51520
MARS: 4141
QARS: 5859
RARS: 5917"
Figure legend: "Northern blot validation of mascRNA in the RIP samples. 5S rRNA from the RIP samples was used as an internal control."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "Gel shift assay of biotin-labeled mascRNA with the cytosol in the presence or the absence of a QARS antibody."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Biotin-labeled mascRNA (sense (S) and antisense (AS)) and the acceptor stem mutants (Mut1-8) were used to pulldown the RNA-protein complexes. Top panel: western blotting of QARS in the pulldown samples. β-Tubulin was used as an cytosolic input control for the pulldown. Bottom panel: Biotin detection of the biotin-labeled mascRNA and the mutants.   Biotin-labeled mascRNA (sense (S) and antisense (AS)) and the anticodon stem-loop mutants (Anti-Mut1-9) were used to pulldown the RNA-protein complexes. Top panel: western blotting of QARS in the pulldown samples. β-Tubulin was used as an cytosolic input control for the pulldown. Bottom panel: Biotin detection of the biotin-labeled mascRNA and the mutants.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Gel shift assay of biotin-labeled mascRNA or the mutants (Anti-Mut1 and Mut1) with the cytosol."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Northern blot analysis of mascRNA in mascRNA overexpressing HEK cells (mascRNA) and the cells expressing the scrambled RNA (Scramble). 5S rRNA was used as an internal control."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "qRT-PCR analysis of MALAT1 (total RNAs used as templates) in mascRNA overexpressing (mascRNA) and the scrambled RNA expressing (Scramble) cells."
NCBI gene IDs:
"MALAT1: 378938
mascRNA: 109136578"
Figure legend: "Detection of the newly synthesized proteins in HEK cells stably expressing mascRNA (mascRNA) or the scrambled RNA (Scramble). Left: biotin detection of the labeled nascent proteins; right: coomassie staining of total proteins.   Quantification of the levels of the labeled nascent proteins in Panel C .   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Northern blot analysis of mascRNA in HEK cells transiently transfected with in vitro synthesized eGFP RNA fragment or mascRNA. 5S rRNA was used as an internal control."
NCBI gene IDs:
"5S rRNA: 
eGFP: 
mascRNA: 109136578"
Figure legend: "Detection of the newly synthesized proteins in HEK cells transiently transfected with in vitro synthesized eGFP RNA fragment or mascRNA. Left: biotin detection of the labeled nascent proteins; right: coomassie staining of total proteins.   Quantification of the levels of the labeled nascent proteins in Panel F.   "
NCBI gene IDs:
"eGFP: 
mascRNA: 109136578"
Figure legend: "Northern blot analysis of mascRNA in mascRNA overexpressing (mascRNA), and the mutants (Anti-Mut1 and Mut1) or the scrambled RNA (Scramble) expressing HEK cells. 5S rRNA was used as an internal control."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "Detection of the newly synthesized proteins in HEK cells stably expressing mascRNA (mascRNA), the mutants (Anti-Mut1 and Mut1) or the scrambled RNA (Scramble). Left: biotin detection of the labeled nascent proteins; right: coomassie staining of total proteins.   Quantification of the levels of the labeled nascent proteins in Panel I.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Decay of the pulse Biotin-labeled proteins in HEK cells stably expressing mascRNA (mascRNA) or the scrambed RNA (Scramble)"
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Western blot detection of ARS proteins in mascRNA overexpressing (mascRNA) or the scrambled RNA expressing (Scramble) cells. β-Tubulin was used as a loading control.   Quantification of the protein levels in Panel A.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Western blot detection of ARS proteins in HEK cells stably expressing mascRNA (mascRNA), the mutants (Anti-Mut1 and Mut1) or the scrambled RNA (Scramble). β-Tubulin was used as a loading control."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Western blot detection of ARS proteins in HEK cells stably expressing mascRNA (mascRNA), the mutants (Anti-Mut1 and Mut1) or the scrambled RNA (Scramble). β-Tubulin was used as a loading control. Quantification of the protein levels in Panel C."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "qRT-PCR analysis of MALAT1 RNA levels in MALAT1 knockdown and the control cells."
NCBI gene IDs:
"MALAT1: 378938"
Figure legend: "Northern blot analysis of mascRNA in MALAT1 knockdown and the control cells. 5S rRNA was used as an internal control."
NCBI gene IDs:
"5S rRNA: 
MALAT1: 378938
mascRNA: 109136578"
Figure legend: "Western blot detection of ARS proteins in MALAT1 knockdown and the control cells.   Quantification of the protein levels in Panel G.   "
NCBI gene IDs:
"MALAT1: 378938"
Figure legend: "qRT-PCR analysis of GARS and QARS mRNAs in mascRNA overexpressing (mascRNA) or the scrambled RNA expressing (Scramble) cells."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Western blot detection of ARS proteins in mascRNA overexpressing (mascRNA) or the scrambled RNA expressing (Scramble) cells, before and after emetine and cycloheximide treatment. β-Tubulin was used as a loading control.   Quantification of the relative QARS protein levels in Panel J.   Quantification of the relative GARS protein levels in Panel J.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Western blot detection of endogenous and FLAG-tagged ARS proteins in HEK cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. β-Tubulin was used as a loading control."
NCBI gene IDs:
"GARS: 2617
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in HEK cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins.   Quantification of the labeled nascent protein levels in Panel B.   "
NCBI gene IDs:
"GARS: 2617
QARS: 5859"
Figure legend: "Western blot detection of FLAG-tagged ARS proteins in mascRNA overexpressing cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. β-Tubulin was used as a loading control."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in mascRNA overexpressing cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins.   Quantification of the labeled nascent protein levels in Panel E.   "
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Western blot detection of FLAG-tagged ARS proteins in mascRNA anticodon stem-loop mutant Anti-Mut1 expressing cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. β-Tubulin was used as a loading control."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in mascRNA Anti-Mut1 expressing cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in mascRNA Anti-Mut1 expressing cells transiently transfected with PQCXIP vector (PQ), QARS expressing (QARS) or GARS expressing (GARS) plasmid. Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins. Quantification of the labeled nascent protein levels in Panel H."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Western blot detection of FLAG-tagged ARS proteins in MALAT1 knockdown cells with stable QARS (QARS) or GARS (GARS) overexpression or without (PQ). β-Tubulin was used as a loading control."
NCBI gene IDs:
"GARS: 2617
MALAT1: 378938
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in wild-type HEK cells (WT) and MALAT1 knockdown cells (sh-MALAT1), or MALAT1 knockdown cells with stable QARS (QARS) or GARS (GARS) overexpression or without (PQ). Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins."
NCBI gene IDs:
"GARS: 2617
MALAT1: 378938
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in wild-type HEK cells (WT) and MALAT1 knockdown cells (sh-MALAT1), or MALAT1 knockdown cells with stable QARS (QARS) or GARS (GARS) overexpression or without (PQ). Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins. Quantification of the labeled nascent protein levels in Panel K."
NCBI gene IDs:
"GARS: 2617
MALAT1: 378938
QARS: 5859"
Figure legend: "Detection of the newly synthesized proteins in QARS (shQARS) or GARS (shGARS) knockdown cells and the control cells stably overexpressing mascRNA (mascRNA) or expressing the scrambled RNA (Scramble). Left, biotin detection of the labeled nascent proteins; right, coomassie staining of total proteins.   Quantification of the labeled nascent protein levels in Panel M.   "
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Northern blotting of mascRNA and 5S rRNA in the lysates of HEK cells before and after FBS starvation."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "Northern blotting of mascRNA and 5S rRNA in the lysates of human embryonic stem cells on different days of differentiation."
NCBI gene IDs:
"5S rRNA: 
mascRNA: 109136578"
Figure legend: "Soft agar colony formation assay of HEK, HeLa or MCF-7 cells overexpressing mascRNA or expressing the the scrambled RNA.   Quantification of stained colones in Panel C.   "
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Soft agar colony formation assay of HEK cells overexpressing mascRNA, or expressing the mutants (Anti-Mut1 and Mut1) or the the scrambled RNA (Scramble)."
NCBI gene IDs:
"mascRNA: 109136578"
Figure legend: "Soft agar colony formation assay of QARS (shQARS) or GARS (shGARS) knockdown cells and the control cells stably overexpressing mascRNA (mascRNA) or expressing the the scrambled RNA (Scramble)."
NCBI gene IDs:
"GARS: 2617
mascRNA: 109136578
QARS: 5859"
Figure legend: "Soft agar colony formation assay of MALAT1 knockdown cells (shMALAT1) and the control cells (Control) with stable QARS (QARS) or GARS (GARS) overexpression or without (PQ)."
NCBI gene IDs:
"GARS: 2617
MALAT1: 378938
QARS: 5859"
Figure legend: "(A) The graphs depict the  Aβ  profiles generated by WT or mutant PSEN1 MEF  cell lines transduced with adenovirus  encoding APPC99. ELISA  quantified secreted Aβ37, Aβ38, Aβ40  and Aβ42  peptide levels are shown as % of total Aβ  (sum of the four peptides). The blue, orange, green and purple dotted lines indicate the levels of Aβ37, Aβ38, Aβ40  and Aβ42  peptides in the WT Aβ  profile, respectively. Data are presented as mean ± SD, N ≥ 3 independent experiments."
NCBI gene IDs:
"APP: 351
PSEN1: 5663"
Figure legend: "(B) GSEC processivity levels for the cleavage of APPC99 determined in WT and mutant PSEN1/GSEC  cell lines. The mutant Aβ(37+38)/(40+42) ratios are shown as % of the WT cell line. Data presented as mean ± SD, N ≥ 3 independent experiments. As reference, a dotted line was drawn to indicate 10% (WT levels). One-way ANOVA followed by Dunnett's post-hoc test with comparison to WT was used to determine statistical significance (P &lt; 0.05); **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001."
NCBI gene IDs:
"PSEN1: 5663"
Figure legend: "(A)  Aβ profiles  generated by WT or mutant PSEN1 MEFs  lines treated with 1 µM GSM III. The chemical structure of the bicyclic heterocycle  GSM III  is depicted. Data are presented as mean ± SD, N ≥ 3 independent experiments. The blue, orange, green and purple dotted lines indicate the levels of Aβ37, Aβ38, Aβ40  and Aβ42  peptides in the WT  Aβ profile, respectively."
NCBI gene IDs:
"PSEN1: 5663"
Figure legend: "(D) Aβ profiles generated by WT or mutant PSEN1  cell lines overexpressing APPC99 and treated with increasing concentrations of GSM III. Data are presented as mean ± SD, N = 2 independent experiments."
NCBI gene IDs:
"APP: 351
PSEN1: 5663"
Figure legend: "Analysis of mutant APP  substrates with regards to their effects on the response of GSEC  to the imidazole-based GSM III. Aβ profiles secreted by HEK293T  cells transiently expressing WT or mutant APPC99 substrates in presence of vehicle (DMSO) or 1 µM GSM III were analysed by ELISA  (D) ELISA-based Aβ  profiles are presented as % of total Aβ  (Aβ37+ Aβ38+ Aβ40+ Aβ42) (mean ± SD, N ≥ 3); One-way ANOVA followed by Dunnett's post-hoc test with comparison to WT was used to determine statistical significance; ****P &lt; 0.0001. F(DFn, DFd): F (4, 17) = 71.68."
NCBI gene IDs:
"APP: 351"