EMBO/SourceData huggingface dataset:
Figure legend: "C RIP experiments performed using anti-myc antibodies and extracts from cells as in (A) followed by qRT-PCR analysis of G-rich and polyA+ tiTERRA. Values correspond to fraction of input RNA expressed as fold increase over an untagged (unt) control tiTEL strain (CAF545). Bars and error bars are averages and SD from three independent experiments. * P &lt; 0.05, ** P &lt; 0.01 (relative to unt; two-tailed Student's t test)."
NCBI gene IDs:
"TERRA: "
Figure legend: "using HeLa cells transfected with non-targeting control (CTRL) or SPRTN siRNAs."
NCBI gene IDs:
"SPRTN: 83932"
Figure legend: "Mass spectrometry-based analysis of formaldehyde-induced SUMOylation changes. His10-SUMO2 conjugates from HeLa/His10-SUMO2 cells subjected or not to formaldehyde were purified on Ni-NTA under stringent conditions and analyzed by mass spectrometry. All proteins displaying significant upregulation of SUMOylation in response to formaldehyde treatment were subjected to network analysis using the STRING database, at the default interaction confidence setting of 0.4. Proteins not connected to the network were omitted."
NCBI gene IDs:
"His10: 
SUMO2: 6613"
Figure legend: "SUMO2 conjugates from HeLa/His10-SUMO2 cells subjected or not to formaldehyde or heat stress for 1 h were purified and immunoblotted with indicated antibodies."
NCBI gene IDs:
"His10: 
SUMO2: "
Figure legend: "HeLa cells were transfected with non-targeting control (CTRL) or DNMT1 siRNAs, exposed or not to 5-azadC and collected 2 h later. Chromatin-enriched fractions were immunoblotted with antibodies to SUMO2/3, DNMT1 and MCM6 (loading control)."
NCBI gene IDs:
"DNMT1: 1786"
Figure legend: "Proliferative capacity of HeLa/GFP-DNMT1 cells transfected with indicated siRNAs and exposed to the indicated 5-azadC doses for 2 h was assayed by measuring cell proliferation with the SRB assay (mean±SEM; n=3 independent experiments; ***p&lt;0.001, Student's t-test)."
NCBI gene IDs:
"GFP: 
DNMT1: 1786"
Figure legend: "Extracts of HeLa/GFP-DNMT1 cells treated with 5-azadC for the indicated times were subjected to GFP immunoprecipitation (IP) under denaturing conditions followed by immunoblotting with SUMO2/3 and GFP antibodies."
NCBI gene IDs:
"GFP: 
DNMT1: 1786"
Figure legend: "HeLa cells were subjected to consecutive rounds of transfection with DNMT1 siRNA targeting the UTR and expression plasmid encoding WT or catalytically inactive (CI) GFP-DNMT1. Cells were then left untreated or incubated with 5-azadC for 2 h, and SUMOylation of GFP-DNMT1 was analyzed Asterisk denotes unmodified GFP-DNMT1 recognized by the SUMO2/3 antibody due to weak cross-reactivity."
NCBI gene IDs:
"GFP: 
DNMT1: 1786"
Figure legend: "Mass spectrometry analysis of 5-azadC-induced SUMOylation changes. His10-SUMO2 conjugates from HeLa/His10-SUMO2 cells subjected or not to 5-azadC were purified on Ni-NTA under stringent conditions and analyzed by mass spectrometry. Proteins displaying significantly altered SUMOylation in response to 5-azadC treatment relative to the control condition were visualized through two-sample t-testing using permutation-based FDR to achieve q-values of &lt;0.05"
NCBI gene IDs:
"His10: 
SUMO2: "
Figure legend: "SUMO target proteins displaying altered SUMOylation upon DNMT1 knockdown are shown. Both control (CTRL) and DNMT1 siRNA-transfected cells were treated with 5-azadC."
NCBI gene IDs:
"DNMT1: 1786"
Figure legend: "HeLa cells stably expressing GFP-DNMT1 and transfected with indicated siRNAs were left untreated or exposed to 5-azadC for 2 h. Cells were then preextracted in stringent preextraction buffer and fixed at the indicated time points after 5-azadC removal. Mean detergent-resistant GFP signal was determined by quantitative image analysis (>6000 cells analyzed per condition). Data from a representative experiment are shown."
NCBI gene IDs:
"GFP: 
DNMT1: 1786"
Figure legend: "Proliferative capacity of HeLa/GFP-DNMT1 cells transfected with control or UBC9 siRNAs and exposed to the indicated 5-azadC doses for 2 h was assayed by measuring cell proliferation with the SRB assay (mean±SEM; n=3 independent experiments; ***p&lt;0.001, Student's t-test)."
NCBI gene IDs:
"GFP: 
DNMT1: 1786
UBC9: 7329"
Figure legend: "Proliferative capacity of HeLa cells transfected with SPRTN or ZNF451 siRNAs and exposed to indicated 5-azadC doses for 2 h was assayed"
NCBI gene IDs:
"SPRTN: 83932
ZNF451: 26036"
Figure legend: "HeLa cells stably expressing GFP-DNMT1 were pre-incubated or not with SUMO-E1i for 30 min, and where indicated 5-azadC was added to the medium for an additional 60 min. Cells were then processed for GFP immunoprecipitation (IP) followed by immunoblotting for the indicated proteins."
NCBI gene IDs:
"GFP: 
DNMT1: 1786"
Figure legend: "Representative images of U2OS cells stably expressing GFP-SPRTN or GFP-ACRC that were transfected with control (CTRL) or UBC9 siRNAs, exposed to formaldehyde in the presence or absence of ubiquitin E1 enzyme (UBA1) inhibitor TAK-243 (Ub-E1i) as indicated and fixed one h later. Scale bar, 10 μm."
NCBI gene IDs:
"UBC9: 7329"
Figure legend: "Quantification of data showing proportion of U2OS/GFP-SPRTN cells displaying nuclear GFP-SPRTN foci (mean±SEM; at least 100 cells quantified per condition per experiment; n=3 independent experiments). As in (C), but showing proportion of U2OS/GFP-ACRC cells displaying nuclear GFP-ACRC foci (mean±SEM; at least 100 cells quantified per condition per experiment; n=3 independent experiments)."
NCBI gene IDs:
"GFP: 
ACRC: 93953
SPRTN: 83932"
Figure legend: "U2OS cells expressing GFP-ACRC were exposed to the indicated genotoxic agents (formaldehyde: 600 μM, 1 h; UV: 20 J/m2, 6 h recovery; hydroxyurea (HU): 2mM, 24 h; mitomycin C (MMC): 40 ng/mL, 24 h), preextracted and fixed, and analyzed by microscopy. Representative images are shown. Scale bar, 10 μm."
NCBI gene IDs:
"GFP: 
ACRC: 93953"
Figure legend: "HeLa cells transfected with plasmids encoding GFP alone or indicated GFP-ACRC alleles were subjected to GFP IP under denaturing conditions. Beads were incubated with recombinant polySUMO22-8 chains, washed extensively and processed for immunoblotting with SUMO2/3 and GFP antibodies."
NCBI gene IDs:
"GFP: 
ACRC: 93953"
Figure legend: "Representative images of U2OS cells expressing indicated GFP-ACRC alleles that were treated with 5-azadC in the presence or absence of SUMO inhibitor (SUMO-E1i), fixed 2 h later and immunostained with DNMT1 antibody. Scale bar, 10 μm.   Quantification of data in (G), showing proportion of cells displaying GFP-ACRC co-localization with DNMT1 foci (mean±SEM; at least 100 cells quantified per condition per experiment; n=3 independent experiments).   "
NCBI gene IDs:
"GFP: 
ACRC: 93953"
Figure legend: "HeLa cells transfected with plasmids encoding GFP alone, GFP-ACRC or GFP-tagged C. elegans GCNA-1 were subjected to GFP IP under denaturing conditions. Beads were incubated with recombinant polySUMO23-8 chains, washed extensively and processed for immunoblotting with SUMO2/3 and GFP antibodies."
NCBI gene IDs:
"GFP: 
ACRC: 93953
GCNA-1: 175850"
Figure legend: "GFP expression driven by the gcna-1 promoter was observed in germ cells, proliferating embryos and young larvae but not in post-mitotic tissues in the head Scale bars, 50 µm."
NCBI gene IDs:
"gcna-1: 175850"
Figure legend: "Formaldehyde survival of wild type (wt), dvc-1, gcna-1 loss-of-function (lof) and gcna-1; dvc-1 double mutant C. elegans (mean±SEM; n=4 independent experiments)."
NCBI gene IDs:
"dvc-1: 179554
gcna-1: 175850"
Figure legend: "Cisplatin survival of wild type, dvc-1, gcna-1 and gcna-1; dvc-1 double mutant C. elegans (mean±SEM; n=3 independent experiments)."
NCBI gene IDs:
"dvc-1: 179554
gcna-1: 175850"
Figure legend: "Formaldehyde survival of gcna-1 deletion (del) and E364Q mutant C. elegans (mean±SEM; n=2 independent experiments)."
NCBI gene IDs:
"gcna-1: 175850"
Figure legend: "Formaldehyde survival of C. elegans grown on L4440 control (CTRL) or smo-1 RNAi bacteria (mean±SEM; n=2 independent experiments)."
NCBI gene IDs:
"smo-1: 266820"
Figure legend: "Formaldehyde survival of C. elegans grown on L4440 control (CTRL) or gei-17 RNAi bacteria (mean±SEM; n=2 independent experiments)."
NCBI gene IDs:
"gei-17: 172733"
Figure legend: "Formaldehyde survival of wild type and gcna-1 deletion (del) mutant C. elegans grown on L4440 control (CTRL) or smo-1 RNAi bacteria (mean±SEM; n=2 independent experiments)."
NCBI gene IDs:
"gcna-1: 175850
smo-1: 266820"
Figure legend: "LC-MS/MS measurements of m6A levels in total RNA (B) or in poly(A)+ RNA (C) upon KD of predicted methyltransferases in Drosophila S2R+ cells. m6A abundance in total RNA is significantly reduced when Mettl5 is depleted, while its depletion has no effect on m6A level in mRNA. As expected, the KD of Mettl3 and Mettl14 reduce m6A levels in mRNA. Bar chart represents the mean ± standard deviation of three technical measurements from three biological replicates. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001 (two-tailed t-test)."
NCBI gene IDs:
"Mettl5: 40096
Mettl14: 34138
Mettl3: 42844"
Figure legend: "Developmental expression of Mettl5 transcript assayed by RT-qPCR analysis. The figure shows mean ± standard deviation of three technical measurements from three biological replicates."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "In situ hybridization of Mettl5 transcript at different embryonic stages. The central nervous system (CNS) is highlighted in the schematics. Data retrieved from FlyExpress 7. (http://www.flyexpress.net/search.php?type=image&amp;search=FBgn0036856)."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "LC-MS/MS measurements of m6A levels in total RNA of WT and Mettl5 mutant flies. Bars represent mean ± standard deviation of measurements of three biological replicates. ***P&lt;0.001 (two-tailed t-test)."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "Mature rRNA analysis on ethidium-stained denaturing agarose gels. The same amounts of total RNA extracted from the indicated flies, and from S2R+ cells depleted or not of Mettl5, were loaded. The 28S/18S ratio was established by densitometry."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "Analysis of 18S rRNA maturation in WT and mutant flies (Mettl5fs). Total RNA extracted from the indicated animals was resolved on denaturing agarose gels and processed for northern blotting with specific probes (complementary to ITS1 or ITS2 sequences). The pre-rRNAs detected accumulate to normal levels indicating that processing is unaffected."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "Trmt112 depletion reduces m6A levels in total RNA from Drosophila S2R+ cells. Mean ± standard deviation of three technical measurements from three biological replicates. *P &lt; 0.05 (two-tailed t-test)."
NCBI gene IDs:
"Trmt112: 40343"
Figure legend: "Representative trajectories of WT and Mettl5 mutant flies analysed by the Buridan's Paradigm. The blue lines indicate when the fly stops and changes direction. Orientation evaluated with the help of the Buridan's paradigm for Mettl5 mutant flies. Orientation was measured as the angular deviation from the straight path needed to move from one landmark to another in the arena. Number of flies tested per genotype: 30. Mean ± standard deviation. Shapiro-Wilk test was used to test for normal distribution in each group. Normally distributed groups were tested by t-test. Due to multiple comparison Bonferroni correction was applied. (p&lt;0.05 = *; p&lt;0.0001=****)."
NCBI gene IDs:
"Mettl5: 40096"
Figure legend: "A, B Data were normalised using the control group and are represented as fold-change (FC) of the mean fluorescence intensity (MFI). Macrophage  cells were obtained from human  donors (n = 4 biological replicas) and incubated with medium (CON) or recombinant human IL-10 (hIL-10r), MPN    wild-type (WT) or MPN    expressing IL-10  ORF (WT_IL10). Data are represented as mean ± Standard Error of the Mean (SEM). Statistical analysis was performed using one-way ANOVA + post-hoc Tukey multiple comparison test (*P &lt; 0.05; **P &lt; 0.001, ***P &lt; 0.0001)."
NCBI gene IDs:
"IL-10: 3586
IL10: 3586"
Figure legend: "(A) EC-50 (molar, M) for human IL-10 recombinant protein (hIL-10r) and  IL-10 WT (IL-10 ORF) and different variants (MutM, hIL-10r, Mut1, Mut2, Mut3, MutSC1, and MutSC2) expressed by M. pneumoniae. Data are represented as mean ± SD. Statistical comparison was done by One-way ANOVA + post-hoc Bonferroni multiple comparison test, using the IL-10 ORF condition as a reference (*P &lt; 0.05)."
NCBI gene IDs:
"IL-10: 3586"
Figure legend: "(B) Fold-change (FC) in expression  levels (fg/CFU) of IL-10 variants Mut3 and MutSC1 secreted to the medium normalised by expression  level of  IL-10 ORF (ORF). Statistical comparison of mean ± SD was performed by One-way ANOVA + Tukey post-hoc test (*P &lt; 0.05)."
NCBI gene IDs:
"IL-10: 3586"
Figure legend: "(C, D) Average and SD values for the FC in the relative  EC-50 values determined by  flow cytometry analysis of phosphorylated STAT3  after a 20-min exposure of the BlaER1 (C) or HAFTL (D) cell lines to  IL-10 ORF (reference) or the mutant MutSC1 or MutSC2 Numbers indicate the average FC ± SD."
NCBI gene IDs:
"IL-10: 3586"
Figure legend: "C57Bl/6 were infected with 107 CFUs of WT or CV8  strains and (B) Inflammatory profile of mouse lungs  inoculated with WT (dark blue), CV8  (light blue), or PBS  (grey). Gene expression  was analysed by RT-qPCR  using gapdh  as an endogenous control (see Materials and Methods). Data are shown as average ± SD of fold-change (FC) in mRNA expression (One-way ANOVA + Tukey post-hoc test; *P &lt; 0.05)."
NCBI gene IDs:
"gapdh: 14433"
Figure legend: "C57Bl/6 were infected with 107 CFUs of WT or CV8  strains and (D) IL-10  secretion by the MPN  WT or CV8  strain coding for hIL-10 WT (ORF), MutSC1, or MutSC2. Data are expressed as the mean ± SD of  IL-10 supernatant concentration (μg/mL) by biomass (protein content in μg/mL) (n=3 biological replicas)."
NCBI gene IDs:
"IL-10: 3586"
Figure legend: "(C) Fold-change (FC) of mRNA  gene expression  of inflammatory markers determined by qPCR. The 2-ΔΔCt method was used to normalise the values, using gapdh  as endogenous control Data are shown as average ± SD of FC. Statistical analyses were performed using unpaired t-test (* P &lt; 0.05; ** P &lt;0.01***; P &lt;0.001, **** P &lt;0.0001). Two replicates were performed and individual symbols are shown (Experiment 1, circle; Experiment 2, square)."
NCBI gene IDs:
"gapdh: 14433"
Figure legend: "HEK293T-ACE2-GFP stable cells were transiently transfected with human IFITM plasmids or vector control for 24 h. D) Graph depicts normalized infection percentage measurements from 2-3 independent experiments, each performed in triplicate, from cells infected Bars represent averages with individual data points shown as circles. Error bars represent SD. #p&lt;0.05 compared to vector control by ANOVA followed by Tukey's multiple comparisons test."
NCBI gene IDs:
"GFP: 
ACE2: 59272"
Figure legend: "HEK293T-ACE2-GFP stable cells were transiently transfected with mouse (m) IFITM plasmids or vector control for 24 h. C) Graph depicts normalized infection percentage measurements from 2-3 independent experiments, each performed in triplicate, from cells infected Bars represent averages with individual data points shown as circles. Error bars represent SD. #p&lt;0.05 compared to vector control by ANOVA followed by Tukey's multiple comparisons test."
NCBI gene IDs:
"GFP: 
ACE2: 59272"
Figure legend: "U2OS cells transfected with EGFP and the indicated IFITM3 constructs or vector control were mixed Calu-3 cells transfected with mCherry and SARS-CoV-2 Spike (S) or vector control. Cells were co-cultured for 24 h and imaged by fluorescent microscopy to visualize EGFP and mCherry as well as nuclei via Hoechst dye. A) Representative example fluorescent imaging of the indicated co-cultures showing the presence of EGFP/mCherry double positive cell syncytia dependent upon S expression as well as varying effects of the IFITM3 constructs. Scale bar indicates 50 μm. B) Quantification of nuclei per EGFP/mCherry double positive cell syncytia in co-cultures involving the indicated IFITM3 constructs as compared to vector control. Bars depict averages of three independent experiments with circles representing the individual data points. For each condition, nuclei within 50-75 individual syncytia were counted. Error bars represent SD. #p&lt;0.05 compared to vector control by ANOVA followed by Tukey's multiple comparisons test. "
NCBI gene IDs:
"EGFP: 
mCherry: 
IFITM3: 10410"
Figure legend: "HEK293T-ACE2-GFP stable cells were transiently transfected with human IFITM plasmids or vector control plus TMPRSS2 or vector control for 24 h. B) Graph depicts normalized infection percentage measurements from 3 independent experiments, each performed in triplicate, from cells infected as in (B). Bars represent averages with individual data points shown as circles. Error bars represent SD. The blue dotted line is added to the graph to assist with comparisons to vector control levels of infection. #p&lt;0.05 by ANOVA followed by Tukey's multiple comparisons test. NS, not significant. Not indicated, additional comparisons of interest with p&lt;0.05 include IFITM3 (-) vs. IFITM3-Y20 (-), IFITM3∆59-68 (-), and IFITM3-S61A,N64A,T65A (-)."
NCBI gene IDs:
"GFP: 
ACE2: 59272
IFITM3: 10410
TMPRSS2: 7113"
Figure legend: " (C) qRT-PCR of Reno1, Cox10as1, and lnc-Nr2f1 in ES cells (top) and following eight days of neuronal differentiation (bottom), following knockdown using two different shRNAs, normalized to sh-NT. Mean ± SEM is shown for 3-6 independent experiments, * P&lt;0.05, ** P&lt;0.01 (unpaired two sample t-test). "
NCBI gene IDs:
"Cox10as1: 66456
Reno1: 100042332
lnc-Nr2f1: 13865"
Figure legend: " (A) Immunostaining using anti-TUJ1 antibody of ES-cell-derived neurons following infection with either non-targeting shRNA (sh-NT), or two different shRNAs targeting Reno1. Scale bar: 200µm. (B) Quantification of cell numbers and neurite lengths of ten images of non-overlapping fields for each shRNA. Mean ± SEM is shown, ** P&lt;0.01 (unpaired two sample t-test). "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (C) Mean expression levels of genes which were down- or upregulated following knockdown (KD) of Reno1 or lnc-Nr2f1 during an eight time point neuronal differentiation and maturation of mouse ES cells: Neurons1 corresponds to day in vitro (DIV)1; Neurons2 - DIV7; Neurons3 - DIV16; Neurons4 - DIV21; and Neurons5 - DIV28 (Hubbard et al., 2013). "
NCBI gene IDs:
"Reno1: 100042332
lnc-Nr2f1: 13865"
Figure legend: "(D) Transcriptional response of cell cycle and neuron-related genes. Heatmaps show log2FC of cells infected with shRNAs targeting Reno1 or lnc-Nr2f1 compared to cells infected with sh-NT, following eight days of neuronal differentiation, and of day eight of differentiation compared to ES cells during differentiation of WT cells."
NCBI gene IDs:
"Reno1: 100042332
lnc-Nr2f1: 13865"
Figure legend: " (A) Reno1 locus in six vertebrate species. Shaded region indicates the Reno1 locus. Blue gene models are from RefSeq or Ensembl, red gene models are based on PLAR transcript reconstructions (Hezroni et al., 2015). RNA-seq datasets from indicated tissues were taken from publicly-available datasets: BodyMap (Li et al, 2017a) (mouse), Reference Epigenome (human), FAANG (sheep), SRP011985 (opossum), SRP016501 (chicken), DRP000627 (coelacanth). RNA-seq data was scaled separately in each species to the highest coverage in the window, except for opossum and chicken, where a custom upper threshold was set as indicated. "
NCBI gene IDs:
"Reno1: 100042332
Reno1: 119545628"
Figure legend: " (B) The mouse Reno1 locus. Expression levels during neuronal differentiation of ES cells, in different cellular fractions of ES cells (data from (Engreitz et al, 2016)) and motor neurons (GSE90913), and in forebrain during different stages of embryonic development (data from the ENCODE project). Each set of tracks was normalized separately. "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (C) RENO1 locus in human. Expression levels in dorsolateral prefrontal cortex across six age groups, and in different cellular fractions in fetal and adult cells. Each set of tracks was normalized separately. "
NCBI gene IDs:
"RENO1: 119545628"
Figure legend: " (D) Reno1 smFISH signal (red) and DAPI staining (blue) in WT and Reno1m/m cells at day eight of neuronal differentiation, imaged using 100x objective. "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (E) Expression levels of Reno1 and Bahcc1 in 30 adult and embryonic tissues. Data from the ENCODE project, quantification was done using RSEM (Li &amp; Dewey, 2011). "
NCBI gene IDs:
"Reno1: 100042332
Bahcc1: 268515"
Figure legend: "(F) Targeted Chromosome Conformation Capture (4C) using the Reno1 promoter as bait. Top, smoothed trend lines and raw counts of the contact profile in ES cells (red) or differentiated neurons (black); bottom, domainogram showing mean contact per fragment end for a series of window sizes."
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: "(B) Expression levels of Reno1 (both the spliced and unspliced isoforms) and Bahcc1 following four days of differentiation of the indicated cells. Mean ± SEM is shown for 3-4 independent experiments, * P&lt;0.05, ** P&lt;0.01 (unpaired two sample t-test)."
NCBI gene IDs:
"Reno1: 100042332
Bahcc1: 268515"
Figure legend: " (A). Heatmap showing log2-transformed fold changes of Reno1 depleted cells compared to their respective controls. All genes which were significantly differentially expressed (P&lt;0.05) in any of the treatments are shown. (B) Boxplots indicating the median, quartiles, and 5th and 95th percentiles of changes in expression levels of Reno1m/m cells compared to WT cells, for genes with significant (P&lt;0.05) change in cells where Reno1 was perturbed with the indicated method. Number of genes in each group is indicated in the x axis. ** P&lt;0.01 (two-sided Wilcoxon rank sum test). "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (C) Mean expression levels of genes in genes which were significantly (P&lt;0.05) dysregulated by at least two out of three Reno1 perturbations at day 2 in fig. 5A (n= 168 down-regulated and 94 up-regulated), during an eight time point neuronal differentiation and maturation of mouse ES cells: Neurons1 corresponds to day in vitro (DIV)1; Neurons2 - DIV7; Neurons3 - DIV16; Neurons4 - DIV21; and Neurons5 - DIV28 (Hubbard et al., 2013). "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (D) Expression levels of Reno1 following Dox addition in WT and Reno1m/m cells. Mean ± SEM is shown for three independent experiments, * P&lt;0.05 (unpaired two sample t-test). "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (E) Cell count of WT and Reno1m/m cells following four days of differentiation, with or without Dox addition. Cells were harvested from one well of a 6-well plate, and counted using Orflo MOXI Z Mini Automated Cell Counter. Mean ± SEM is shown for three independent experiments. * P&lt;0.05 (unpaired two sample t-test). "
NCBI gene IDs:
"Reno1: 100042332"
Figure legend: " (F-G) Boxplots indicating the median, quartiles, and 5th and 95th percentiles of changes in expression levels of WT (F) or Reno1m/m (G) cell treated with Dox for the first two days of differentiation compared to untreated cells, in genes which were significantly (P&lt;0.05) downregulated (n=252) or upregulated (n=223) by at least two out of three Reno1 perturbations at day 2 in fig. 5A, compared to all genes (n=16,511). ** P&lt;0.01 (two-sided Wilcoxon rank sum test). "
NCBI gene IDs:
"Reno1: 100042332"