EMBO/SourceData huggingface dataset:
Figure legend: "The effect of the pwwp1, pwwp2, and pwwp3 mutations on the silencing of solo LTR, SDC, and FWA as determined by qPCR. Showing are results of three biological replicates with standard deviations."
NCBI gene IDs:
"pwwp2: 841600
pwwp1: 821075
pwwp3: 5008017
FWA: 828658
SDC: 816276"
Figure legend: "(E) The effect of the trb1 mutation and the TRB2 knockdown (TRB2-KD) on the silencing of solo LTR, as determined by qPCR (right panel). The transcript level of TRB2 was evaluated to confirm the TRB2 knockdown (left panel). Error bars represent standard deviations of three biological replicates."
NCBI gene IDs:
"trb1: 841418
TRB2: 836894"
Figure legend: "(B) Heat maps showing differentially transcribed genes and TEs in the arid2/3/4 and epcr1/2 mutants relative to the wild type."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(D) Genome browser snapshots showing the transcript patterns of ROS1 and IBM1 in arid2/3/4 and epcr1/2 relative to the wild type."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397
ROS1: 818224
IBM1: 819952"
Figure legend: "(E) Effects of arid2/3/4 epcr1/2 on the transcript levels of ROS1 and IBM1-L as determined by qPCR. The expression of ROS1 and the longer version of IBM1 (IBM1-L) was evaluated by qPCR. ACT7 was amplified as an internal control. Bars represent SD from two independent experiments, each with three technical replications."
NCBI gene IDs:
"ACT7: 830841
arid2: 826744
epcr1: 829397
ROS1: 818224
IBM1: 819952"
Figure legend: "(A) The interaction of EPCR1 with HAM1 and HAM2. EPCR1-Flag transgenic plants were crossed to HAM1-Myc and HAM2-Myc transgenic plants. The progeny were used to evaluate the interaction between EPCR1 with HAM1 and HAM2 by co-IP."
NCBI gene IDs:
"Flag: 
Myc: 
EPCR1: 829397
HAM1: 836582
HAM2: 825234///830834"
Figure legend: "(B) The interaction of ARID2, ARID3, and ARID4 with HAM1 and HAM2. ARID2-Myc, ARID3-Myc, and ARID4-Myc transgenic plants were crossed to HAM1-Flag and HAM2-Flag transgenic plants and their progeny were used for co-IP."
NCBI gene IDs:
"ARID3: 
Flag: 
Myc: 
ARID4: 843984
ARID2: 826744
HAM1: 836582
HAM2: 830834"
Figure legend: "(C) The interaction of TRB1 with HAM1 and HAM2. TRB1-Flag transgenic plants were crossed to HAM1-Myc and HAM2-Myc transgenic plants and their progeny were used for co-IP."
NCBI gene IDs:
"Flag: 
Myc: 
HAM1: 836582
HAM2: 830834
TRB1: 841418"
Figure legend: "(D) The interaction of HDA9 with ARID2, ARID3, and ARID4. ARID2-Myc, ARID3-Myc, and ARID4-Myc transgenic plants were crossed to HDA9-Flag transgenic plants and their progeny were used for co-IP."
NCBI gene IDs:
"ARID3: 
Flag: 
Myc: 
ARID4: 843984
ARID2: 826744
HDA9: 823594"
Figure legend: "(E) The interaction of HDA6 with EPCR1 and ARID2. EPCR1-Flag and ARID2-Flag transgenic plants were crossed to HDA6-Myc transgenic plants and their progeny were used for co-IP."
NCBI gene IDs:
"Flag: 
Myc: 
ARID2: 826744
EPCR1: 829397
HDA6: 836431"
Figure legend: "(A) Overlap among H4K5 hyperacetylated regions in the arid2/3/4 and epcr1/2 mutants relative to the wild type. The overlap is significant (P=4.410-78) as determined by hypergeometric test. (B) Metaplot of histone H4K5 acetylation levels of TEs in wild type, arid2/3/4, and epcr1/2 plants. Histone H4K5 acetylation levels are represented by normalized reads that obtained in the H4K5Ac ChIP-seq analysis. TSS represents transcription start site and TTS represents transcription termination site. (C) Boxplot of histone H4K5 acetylation levels of TEs in wild type, arid2/3/4, and epcr1/2 plants. Asterisks indicate that H4K5Ac levels is significantly higher in the arid2/3/4 (P=2.4210-13) and epcr1/2 (P=1.5210-8) mutants than in the wild type as determined by Welch's Two-Sample t-test. Horizontal lines represent the median, and the bottom and top of the box represent the 25th and 75th percentile. The whiskers represent data range within 1.5× of the interquantile range."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(D) The nuclei with condensed, intermediate, or decondensed heterochromatin status, as marked by DAPI staining and H3K27me signals. (E) Percentages of nuclei with condensed, intermediate, or decondensed heterochromatin signals in the wild type and the arid2/3/4 mutant. n=113."
NCBI gene IDs:
"arid2: 826744"
Figure legend: "(F) Abundance of Pol IV-dependent siRNAs in the epcr1/2, arid2/3/4, nrpd1, nrpe1, and hda6 mutants relative to the wild type [(Mut-WT)/(Mut+WT)] from chromosome 3 of Arabidopsis."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397
hda6: 836431
nrpd1: 842605
nrpe1: 818591"
Figure legend: "(H) Boxplot showing levels of Pol IV-dependent siRNAs that are up-regulated in the hda6 mutant. Asterisks indicate that siRNA levels in the mutants are significantly (P→0) up-regulated compared to the wild type as determined by paired student t-test. Horizontal lines represent the median, and the bottom and top of the box represent the 25th and 75th percentile. The whiskers represent data range within 1.5× of the interquantile range."
NCBI gene IDs:
"hda6: 836431"
Figure legend: "(A) Metaplot of CG, CHG, and CHH methylation of genes and TEs in the genomes of wild type, epcr1/2, and arid2/3/4 plants. H indicates C, A, or T."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(B) Metaplot of CG, CHG, and CHH methylation on chromosome 3 of Arabidopsis in wild type, epcr1/2, and arid2/3/4 plants."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(D) Boxplot of DNA methylation in the wild type, nrpe1, epcr1/2, and arid2/3/4 mutants. The hypo-DMRs identified in the nrpe1 mutant was defined as RdDM target loci. Asterisks indicate that DNA methylation is significantly (P&lt;0.001) decreased in the mutants as determined by paired student t-test. Horizontal lines represent the median, and the bottom and top of the box represent the 25th and 75th percentile. The whiskers represent data range within 1.5× of the interquantile range."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397
nrpe1: 818591"
Figure legend: "(E) Heat maps of CG, CHG, and CHH methylation at RdDM target loci. The hypo-DMRs identified in the nrpe1 mutant were defined as RdDM target loci and analyzed in the wild type, nrpe1, eprc1/2, and arid2/3/4 mutants. Black and light yellow indicate low methylation and high methylation, respectively."
NCBI gene IDs:
"arid2: 826744
eprc1: 829397
nrpe1: 818591"
Figure legend: "(A) Scatter plots showing the effect of the epcr1/2 and arid2/3/4 mutations on CG, CHG, and CHH methylation at the co-upregulated TEs and genes in the epcr1/2 and arid2/3/4 mutants. TE methylation and gene DNA methylation refer, respectively, to methylation of the TE body and methylation of the 1-kb-gene-promoter region."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(B) Box plots of CG, CHG, and CHH methylation in the wild type, arid2/3/4, and epcr1/2 mutants at the co-upregulated TEs and genes in the arid2/3/4 and epcr1/2 mutants. Asterisks indicate statistical significance as determined by paired student t test. CHG methylation of co-upregulated TEs is significantly increased in arid2/3/4 (P=1.2610-4) and epcr1/2 (P=6.310-9); CHH methylation of co-upregulated genes is significantly decreased in arid2/3/4 (P=3.510-6) and epcr1/2 (P=6.410-7). Horizontal lines represent the median, the bottom and top of the box represent the 25th and 75th percentile. The whiskers represent data range within 1.5× of the interquantile range."
NCBI gene IDs:
"arid2: 826744
epcr1: 829397"
Figure legend: "(C) Overexpression of DRD1 fails to restore the silencing of EPCR1/2 target loci in the epcr1/2 mutant background."
NCBI gene IDs:
"epcr1: 829397
EPCR1: 829397
DRD1: 816136"
Figure legend: "Live imaging of histone-GFP (green) and ParB-mCherry (magenta) expressed in larval salivary gland nuclei using 1151-Gal4. H3.3-GFP levels are increased at the E(spl)-C in the presence of constitutively active Notch, NΔECD (Notch-ON), compared to control Notch-OFF nuclei expressing LacZ (A). ParB-mCherry binds to its cognate int DNA sequence inserted within the E(spl)-C [8,64]. Yellow dotted box contains E(spl)-C and yellow arrow indicates position of E(spl)-C on chromosome. Scale bars (white) = 5 µm. Quantifications of relative fluorescence intensity of histone-GFP and ParB-mCherry across the E(spl)-C in Notch-OFF (upper) and Notch-ON (lower) conditions. Mean+/-SEM; nnuclei = 7, 6, 5, 8, 9, 11 and nglands = 5, 5, 5, 5, 5, 4 where each gland represents a biological replicate (from top to bottom)."
NCBI gene IDs:
"Gal4: 
GFP: 
LacZ: 
mCherry: 
ParB: 
histone: 318847///3771792///3772189///3771959///3772032///3772149///3772163///3772173///3772191///3772198///3772231///3771771///3772374///3771729///3772607///3772619///3772517///3772552///3771723///3772370///3772421///3772489///3772518"
Figure legend: "Live imaging of histone-GFP (green) and ParB-mCherry (magenta) expressed in larval salivary gland nuclei using 1151-Gal4. E(spl)-C in the presence of constitutively active Notch, NΔECD (Notch-ON), compared to control Notch-OFF nuclei expressing LacZ but there is little change in H3-GFP between Notch-OFF and Notch-ON nuclei ParB-mCherry binds to its cognate int DNA sequence inserted within the E(spl)-C [8,64]. Yellow dotted box contains E(spl)-C and yellow arrow indicates position of E(spl)-C on chromosome. Scale bars (white) = 5 µm. Quantifications of relative fluorescence intensity of histone-GFP and ParB-mCherry across the E(spl)-C in Notch-OFF (upper) and Notch-ON (lower) conditions. Mean+/-SEM; nnuclei = 7, 6, 5, 8, 9, 11 and nglands = 5, 5, 5, 5, 5, 4 where each gland represents a biological replicate (from top to bottom)."
NCBI gene IDs:
"Gal4: 
GFP: 
LacZ: 
mCherry: 
ParB: 
histone: 318847///3771792///3772189///3771959///3772032///3772149///3772163///3772173///3772191///3772198///3772231///3771771///3772374///3771729///3772607///3772619///3772517///3772552///3771723///3772370///3772421///3772489///3772518"
Figure legend: "Live imaging of histone-GFP (green) and ParB-mCherry (magenta) expressed in larval salivary gland nuclei using 1151-Gal4. E(spl)-C in the presence of constitutively active Notch, NΔECD (Notch-ON), compared to control Notch-OFF nuclei expressing LacZ (A). with H3.3core-GFP ParB-mCherry binds to its cognate int DNA sequence inserted within the E(spl)-C [8,64]. Yellow dotted box contains E(spl)-C and yellow arrow indicates position of E(spl)-C on chromosome. Scale bars (white) = 5 µm. Quantifications of relative fluorescence intensity of histone-GFP and ParB-mCherry across the E(spl)-C in Notch-OFF (upper) and Notch-ON (lower) conditions. Mean+/-SEM; nnuclei = 7, 6, 5, 8, 9, 11 and nglands = 5, 5, 5, 5, 5, 4 where each gland represents a biological replicate (from top to bottom)."
NCBI gene IDs:
"Gal4: 
GFP: 
LacZ: 
mCherry: 
ParB: 
histone: 318847///3771792///3772189///3771959///3772032///3772149///3772163///3772173///3772191///3772198///3772231///3771771///3772374///3771729///3772607///3772619///3772517///3772552///3771723///3772370///3772421///3772489///3772518"
Figure legend: "Effects from depleting chromatin remodelers and histone chaperones, as indicated (wide range shown in A, on recruitment of Su(H)-GFP in Notch-ON nuclei (expressing NΔECD) of larval salivary glands. Different OSA RNAi stocks used in C are denoted by (1) and (2). In all conditions except Moira RNAi (A) nuclei exhibit a bright accumulation of Su(H)-GFP at a single locus when imaged live. Scale bars (yellow) = 5 µm. Percentage of Notch-ON nuclei retaining a single clear band of Su(H)-GFP when the indicated RNAi is co-expressed with NΔECD. For each genotype, 5 nuclei from each of 10 glands were scored (50 nuclei total). **** A significant fraction of nuclei lost the fluorescent band when core components of the BRM complex were perturbed; p&lt;0.0001, two-tailed Fisher's exact test calculated using the raw (non-percentage) scoring data."
NCBI gene IDs:
"Moira: 41942
OSA: 42130"
Figure legend: "Effects from depleting chromatin remodelers and histone chaperones , BRM complex components shown in C), on recruitment of Su(H)-GFP in Notch-ON nuclei (expressing NΔECD) of larval salivary glands. w RNAi is a control and BrmK804R is expression of dominant-negative Brm. Different OSA RNAi stocks used in C are denoted by (1) and (2). , Snr1 RNAi and BrmK804R expression (C), nuclei exhibit a bright accumulation of Su(H)-GFP at a single locus when imaged live. Scale bars (yellow) = 5 µm. Percentage of Notch-ON nuclei retaining a single clear band of Su(H)-GFP when the indicated RNAi is co-expressed with NΔECD. For each genotype, 5 nuclei from each of 10 glands were scored (50 nuclei total). **** A significant fraction of nuclei lost the fluorescent band when core components of the BRM complex were perturbed; p&lt;0.0001, two-tailed Fisher's exact test calculated using the raw (non-percentage) scoring data."
NCBI gene IDs:
"Brm: 39744
OSA: 42130
Snr1: 40657"
Figure legend: "Chromatin accessibility in Notch-OFF (B) and Notch-ON (NΔECD expression, C) salivary gland nuclei measured by ATAC-qPCR; fold enrichment at the indicated regions compared to a &quot;closed ctrl&quot; region. Expression of dominant-negative Brm, BrmK804R, led to reduced accessibility of E(spl)mβ-HLH and E(spl)m3-HLH enhancer regions in Notch-OFF conditions, and to a more widespread reduction in accessibility in Notch-ON conditions. &quot;Eip78C enh&quot; corresponds to the ecdysone receptor-binding region of the Eip78C enhancer, which is highly accessible but not Notch-responsive; &quot;Rab11 tr&quot; and &quot;Mst87F tr&quot; represent highly and lowly-expressed control genes respectively. Mean +/- SEM; n = 3; * p&lt;0.05 with two-tailed Welch's t-test comparing LacZ and BrmK804R samples."
NCBI gene IDs:
"LacZ: 
Brm: 39744
E(spl)m3-HLH: 43156
E(spl)mβ-HLH: 43152
Eip78C: 40345
Mst87F: 41693
Rab11: 42501"
Figure legend: "Chromatin accessibility in salivary gland nuclei depleted for Su(H) by RNAi, measured by ATAC-qPCR; fold enrichment at the indicated regions compared to a \"closed ctrl\" region. Accessibility is increased across most of the E(spl)-C compared to controls expressing LacZ. Control primer regions are as in B and C. Mean +/- SEM; n = 3; * p&lt;0.05 with two-tailed Welch's t-test compared to LacZ controls."
NCBI gene IDs:
"LacZ: 
Su(H): 34881"
Figure legend: "Effect of brm and Snr1 RNAi on brm and Snr1 cDNA levels respectively, measured by reverse transcription-qPCR in Kc167 cells; percentage cDNA compared to GFP RNAi. The knockdowns are highly effective, with only 1-2% of brm and Snr1 cDNA remaining detectable. Mean +/- SEM; n = 3."
NCBI gene IDs:
"GFP: 
brm: 39744
Snr1: 40657"
Figure legend: "Knockdown of components of the BRM complex reduces Su(H) recruitment both in Notch-OFF (B) and Notch-ON (C) conditions. Fold enrichment of Su(H) occupancy at the indicated positions detected by ChIP, relative to input, in Kc167 cells treated with brm, Snr1 or GFP RNAi as a control. Notch-ON conditions (C) were induced by 30 minutes of EGTA treatment. Mean +/- SEM, n = 3 (B); Mean, n = 2 (C); * p&lt;0.05 with one-tailed student's t-test compared to GFP RNAi control."
NCBI gene IDs:
"GFP: 
brm: 39744
Snr1: 40657"
Figure legend: "Effect of brm RNAi on E(spl)mβ-HLH (mβ) and E(spl)m3-HLH (m3) induction by Notch activation (EGTA treatment) measured by reverse transcription-qPCR; shown as fold difference to lacZ RNAi control. Mean +/- SEM; n = 3."
NCBI gene IDs:
"lacZ: 
brm: 39744
E(spl)m3-HLH: 43156
m3: 43156
E(spl)mβ-HLH: 43152
mβ: 43152"
Figure legend: "Effect of Brm dominant-negative on expression of E(spl)mβ-HLH (mβ) and E(spl)m3-HLH (m3) measured by reverse transcription-qPCR. Expression was analyzed in stable cell lines containing pMT-inducible BrmWT or BrmK804R in the absence (left, uninduced) or presence of copper sulfate (right, Cu2+ induced). The response of E(spl)mβ-HLH and E(spl)m3-HLH to Notch activation (&quot;N-On&quot; = EGTA treatment vs. &quot;N-Off&quot; = PBS control) was reduced in the BrmK804R-expressing cells compared to BrmWT-expressing cells, only when induced with copper (right graph). Mean, n = 2 (left); Mean +/- SEM, n = 3 (right); * p&lt;0.05 with one-tailed student's t-test comparing BrmWT and BrmK804R."
NCBI gene IDs:
"Brm: 39744
E(spl)m3-HLH: 43156
m3: 43156
E(spl)mβ-HLH: 43152
mβ: 43152"
Figure legend: "Knockdown of actin-related subunit, BAP55, reduces Su(H) recruitment in both Notch-OFF (PBS treatment) and Notch-ON (EGTA treatment) conditions. Fold enrichment of Su(H) occupancy at the indicated positions detected by ChIP, relative to input, in Kc167 cells treated with Bap55 or lacZ RNAi as a control. Mean +/- SEM; n = 3; * p&lt;0.05 with one-tailed student's t-test compared to lacZ RNAi control."
NCBI gene IDs:
"lacZ: 
Bap55: 36956
BAP55: 36956"
Figure legend: "Effect of Bap55 RNAi on E(spl)mβ-HLH (mβ) and E(spl)m3-HLH (m3) expression levels measured by reverse transcription-qPCR in Notch-OFF (PBS treatment) and Notch-ON (EGTA treatment) conditions. Expression level of RpII215 is shown as a control gene. Mean +/- SEM; n = 3; * p&lt;0.05 with one-tailed student's t-test compared to lacZ RNAi control."
NCBI gene IDs:
"lacZ: 
Bap55: 36956
E(spl)m3-HLH: 43156
m3: 43156
E(spl)mβ-HLH: 43152
mβ: 43152
RpII215: 32100"
Figure legend: "Nucleosome turnover measured by CATCH-IT-qPCR; fold enrichment over input samples compared to Sec15 tr control region. Su(H)-bound enhancers show increased nucleosome turnover in response to Notch signaling. Notch signaling is activated in Kc167 cells by 6 hours of copper induction of pMT-NICD with copper excluded in the control. Positions of E(spl)-C primers are shown in Fig 3A; the remaining primers are control non-Notch-responsive regions. Mean, n = 2."
NCBI gene IDs:
"Sec15: 42499"
Figure legend: "brm RNAi reduces nucleosome turnover at Notch-responsive regions. CATCH-IT-qPCR results as in A after brm or lacZ RNAi as a control. Mean +/- SEM; n = 5; * p&lt;0.05 with one-tailed student's t-test comparing brm and lacZ RNAi."
NCBI gene IDs:
"lacZ: 
brm: 39744"
Figure legend: "Brm is required for Notch-responsive nucleosome turnover. CATCH-IT-qPCR results after brm or lacZ RNAi as in B and pMT-NICD expression as in A. Mean +/- SEM; n=3; * p&lt;0.05 with two-tailed student's t-test compared to control (lacZ RNAi Notch-ON bars are compared to lacZ RNAi Notch-OFF bars and brm RNAi bars are compared to their respective lacZ RNAi control bars)."
NCBI gene IDs:
"lacZ: 
Brm: 39744"
Figure legend: "brm RNAi reduces incorporation of histone H3.3. V5 ChIP-qPCR in Kc167 cells after lacZ or brm RNAi treatment in cells with H3-V5 or H3.3-V5 expression induced from the pMT promoter by 3 hours of copper treatment, shown as fold enrichment over input samples. Mean +/- SEM; n = 3. * p&lt;0.05 with two-tailed Welch's t-test compared to lacZ RNAi control."
NCBI gene IDs:
"lacZ: 
H3: 
H3.3: 
V5: 
brm: 39744
histone H3.3: 33736"
Figure legend: "C Cold response of cytosolic  free Ca2+  concentration in root cells expressing NES-YC3.6. Superimposition of representative normalized ratio variations for wild-type, oscrt3-1, and the complemented transgenic pOsCRT3 root  cells. The rectangle represents the regions of interest (ROIs) for the ratio measurements. The blue background indicates duration of cold treatment. Bar: 50 μm."
NCBI gene IDs:
"crt3: 9267167
CRT3: 9267167"
Figure legend: "B Yeast two-hybrid analysis to test the OsCRT3-OsCIPK7 interaction. The interactions  between OsCRT3, OsCRT3N, OsCRT3P or  OsCRT3C with  OsCIPK7, OsCIPK7N, OsCIPK7C,  OsCIPK7NAF, or OsCIPK7PPI were verified."
NCBI gene IDs:
"CIPK7: 4333514
CRT3: 9267167"
Figure legend: "(A-B) Expression patterns (blue stain) of GUS reporter genes driven by the SKM1(A) or SKM2(B) promoter in roots of 5-day-old Col-0seedlings."
NCBI gene IDs:
"GUS: 
SKM1: 817121
SKM2: 835702"
Figure legend: "(C) Non-complementation of CLE45 insensitivity of the bam3mutant by BAM3expressed under control of the SKM1promoter (7-day-old seedlings; 2 independent transgenic lines per construct are shown)."
NCBI gene IDs:
"SKM1: 817121
bam3: 827774
BAM3: 827774"
Figure legend: "(D) Full CLE45 sensitivity of skm1or skm2 single and double mutant roots (7-day-old seedlings)."
NCBI gene IDs:
"skm1: 817121
skm2: 835702"
Figure legend: "(E) Failure of SKM1 or SKM2 expressed under control of the BAM3 promoter to complement CLE45 insensitivity of the bam3 mutant (7-day-old seedlings; 3 independent transgenic lines per construct are shown)."
NCBI gene IDs:
"SKM1: 817121
SKM2: 835702
BAM3: 827774
bam3: 827774"
Figure legend: "(F) Gap cell frequency in brxsingle or brxmakr5 double mutants (note: gap cells were absent from Col-0 wild type or makr5single mutants). (G) Illustration of gap cells (arrowheads) in developing protophloem sieve element strands (asterisks) of brxsingle or brxmakr5 double mutants."
NCBI gene IDs:
"makr5: 835364
brx: 840078"
Figure legend: "(D) Expression pattern of GUS reporter gene driven by the MAKR5 promoter in roots of 5-day-old Col-0seedlings. (E) Corresponding transverse sections from different positions along the root meristem."
NCBI gene IDs:
"GUS: 
MAKR5: 835364"
Figure legend: "(H) Complementation of makr5CLE45 insensitivity by MAKR5expression under control of a promoter specific for developing sieve elements (CVP2), but not by expression under control of a companion cell-specific promoter (SUC2) (7-day-old seedlings; 3 independent transgenic lines per construct are shown)."
NCBI gene IDs:
"makr5: 835364
MAKR5: 835364
CVP2: 837048
SUC2: 838877"
Figure legend: "(B) Expression of truncated MAKR5 fusion proteins under control of the MAKR5 promoter in 5-day-old roots (asterisks: protophloem sieve element strands)."
NCBI gene IDs:
"MAKR5: 835364"
Figure legend: "(C) Close up of protophloem sieve element strands expressing truncated MAKR5-CITRINE fusion proteins under control of the UBQ10 promoter."
NCBI gene IDs:
"MAKR5: 835364
UBQ10: 825880"
Figure legend: "(D) Failure of truncated MAKR5 fusion proteins to complement CLE45 insensitivity of the makr5 mutant (7-day-old seedlings; 1-2 independent transgenic lines per construct are shown)."
NCBI gene IDs:
"makr5: 835364
MAKR5: 835364"
Figure legend: "(E) Sensitivity of makr5 mutant roots to continuous brassinolide (BL) treatment (7-day-old seedlings)."
NCBI gene IDs:
"makr5: 835364"
Figure legend: "(F) Expression of MAKR5-BKI1 fusion proteins under control of the MAKR5 promoter in roots of 5-day-old makr5 seedlings."
NCBI gene IDs:
"MAKR5: 835364
makr5: 835364"
Figure legend: "(G) Complementation of CLE45 insensitivity of makr5 roots by BKI1-MAKR5, but not MAKR5-BKI1 fusion protein (7-day-old seedlings; 3 independent transgenic lines per construct are shown)."
NCBI gene IDs:
"MAKR5: 835364
BKI1: 834284"
Figure legend: "(A) Increased expression of BAM3-CITRINE fusion protein under control of the BAM3 promoter in developing protophloem sieve elements (asterisks) of 5-day-old bam3 roots upon CLE45 treatment."
NCBI gene IDs:
"bam3: 827774"
Figure legend: "(B) Expression of BAM3-CITRINE fusion protein under control of the BAM3 promoter in 5-day-old Col-0 roots or makr5 roots."
NCBI gene IDs:
"makr5: 835364"
Figure legend: "(C) Expression of MAKR5-GFP fusion protein under control of the MAKR5 promoter in 5-day-old Col-0 or bam3 roots."
NCBI gene IDs:
"bam3: 827774"
Figure legend: "(D) GUS reporter gene expression driven by the MAKR5 promoter in roots of 5-day-old Col-0seedlings upon CLE45 treatment."
NCBI gene IDs:
"GUS: 
MAKR5: 835364"
Figure legend: "(E) Expression of MAKR5-GFP fusion protein under control of the MAKR5 promoter in 5-day-old roots upon CLE45 treatment (3 h). Note accumulation of MAKR5-GFP in protophloem sieve elements (magnified) and its dependence on functional BAM3 and CLE45. The frequency of representative observations per total observations is indicated."
NCBI gene IDs:
"BAM3: 827774"
Figure legend: "(G-H) Accumulation of MAKR5-BKI1 fusion proteins in protophloem cells upon CLE45 treatment. The frequency of representative observations per total observations is indicated. (I-J) Quantification of fusion protein abundance in developing protophloem cells upon expression under control of the MAKR5 promoter, showing mean total fluorescence (I) and plasma membrane to cytosol signal ratio (J)."
NCBI gene IDs:
"MAKR5: 835364"