EMBO/SourceData huggingface dataset:
Figure legend: "C. RUSH assay for CRB3-GFP  in control (mcherry) and dynactin-inhibited radial glial cells  (CC1-p150-dsRed), electroporated at E.15.5 and imaged at E16.5. Scale bar = 5µm. D. CRB3 localization  at the apical surface  upon release. E. Percentage of cells with 100% of CRB3  signal at the apical surface  upon release. Data information: (D, E) mCherry  (control): N= 3 brains  per timepoint  (361 cells total). CC1-p150: N=3 brains  per timepoint (2 brains  for T0) (268 cells total). Fisher's exact test and Benjamini-Hochberg procedure, *** p ≤ 0.001. All error bars indicate SD."
NCBI gene IDs:
"dsRed: 
mcherry: 
mCherry: 
p150: 1639"
Figure legend: "A. PAX6 and phospho-Histone 3 (p-H3) staining  in WT and LIS1  KO E12.5 brains. Cortices were subdivided into 5 bins of equal size along the radial axis. Scale bar = 25µm. B. Percentage of PH3+/PAX6+ cells located above the ventricular surface of WT and LIS1  KO E12.5 brains. WT: 1192 cells from N=6 brains. LIS1  KO: 589 cells from N=3 brains. Data information: (B, Mann-Whitney U test, *p ≤ 0.05. All error bars indicate SD."
NCBI gene IDs:
"LIS1: 18472"
Figure legend: "C. Phospho-Vimentin  (p-VIM) staining  in WT and LIS1  KO E12.5 brains. Scale bar = 25µm. D. Percentage p-VIM+ cells dividing ectopically, away from the  ventricular surface of WT and LIS1 KO E12.5 brains. WT: 1056 cells from N=6 brains. LIS1  KO: 879 cells from N=3 brains. Data information: D) Mann-Whitney U test, *p ≤ 0.05. All error bars indicate SD."
NCBI gene IDs:
"LIS1: 18472"
Figure legend: "A. CRB3  and PALS1 staining  in WT, RAB6A/B dKO (E15.5) and LIS1  KO (E12.5) brains. Scale bar = 25µm."
NCBI gene IDs:
"LIS1: 18472
RAB6A: 19346"
Figure legend: "B. Quantification of CRB3 staining interruptions along the ventricular boundary of WT (E12.5 and E15.5; N=13 brains), LIS1  KO (E12.5, N=4 brains)) and RAB6A/B dKO (E15.5, N=4 brains). Data information: (B, Kruskal-Wallis test with a Dunn post-hoc test and Benjamini-Hochberg procedure, *p ≤ 0.05, *** p ≤ 0.001. All error bars indicate SD."
NCBI gene IDs:
"LIS1: 18472
RAB6A: 19346"
Figure legend: "C. N-Cadherin staining  in WT, RAB6A/B dKO (E15.5) and LIS1  KO (E12.5) brains. Scale bar = 25µm."
NCBI gene IDs:
"LIS1: 18472
RAB6A: 19346"
Figure legend: "D. Quantification of N-Cadh staining  interruptions along the ventricular boundary of WT (E12.5 and E15.5; N=6 brains), LIS1  KO (E12.5, N=3 brains) and RAB6A/B dKO (E15.5, N=3 brains). Data information: D) Kruskal-Wallis test with a Dunn post-hoc test and Benjamini-Hochberg procedure, *p ≤ 0.05, *** p ≤ 0.001. All error bars indicate SD."
NCBI gene IDs:
"LIS1: 18472
RAB6A: 19346"
Figure legend: "(C-J) All panels show Drosophila 3rd instar larval eye imaginal discs with eyeless-Flippase-induced clones of cells homozygous for EMC3Δ4 labelled by the absence of  ubiGFP (green). The red channel shows ELAV, which labels the nuclei  of  photoreceptor cells. All UAS constructs were expressed under the control of GMR-GAL4. (C) Expression  of UAS-Rh1 (4C5, in blue) and (D)  Na+K+ATPase (A5-C, in blue) is strongly reduced in EMC3Δ4 homozygous mutant cells. (E) Expression of UAS-Xport-A-HA (anti-HA, in blue) and (F) UAS-fan-HA  (anti-HA, in blue) is strongly reduced in  EMC3Δ4 homozygous mutant cells. (G) Expression of UAS-rtv-HA (anti-HA, in blue) and (H) hid (anti-hid, in blue) is increased in  EMC3Δ4 homozygous mutant cells. (I-J) Normal expression of two TA proteins in EMC3Δ4 homozygous mutant cells. (I) UAS-CG8814-HA (anti-HA, in blue) is a TA protein containing a TMD of low hydrophobicity (J) UAS-Syx13-HA is a TA protein with high hydrophobicity TMD. Scale bars represent 10 μm."
NCBI gene IDs:
"Flippase: 32615
EMC3: 34479
eyeless: 43812
GAL4: 855828"
Figure legend: "(B-D) - Immunostaining  of  3rd instar   larval eye imaginal discs with eyeless-Flippase-induced clones of cells homozygous for EMC3Δ4, labelled by the absence of  ubiGFP (green) shows reduced  expression of (B) UAS-HA-Xport-A (anti-HA, in blue) but normal expression of (C) UAS-HA-Xport-A4L (anti-HA, in blue) or (D) UAS-Xport-A4L-HA (anti-HA, in blue) in EMC3  mutant homozygous cells. ELAV (red in B and C) marks photoreceptor cells and Na+K+ATPase (red in D) acts as a positive control for the presence of EMC3Δ4 homozygous mutant clones. The UAS constructs were expressed under the control of GMR-GAL4. Scale bars represent 10 μm."
NCBI gene IDs:
"Flippase: 32615
EMC3: 34479
eyeless: 43812
GAL4: 855828"
Figure legend: "(E-F) Immunostaining of mosaic adult  retina with eyeless-Flippase-induced clones of cells homozygous for EMC3e02662, labelled by the absence of RFP (red), show loss of (E) UAS-HA-Xport-A (anti-HA, in blue) in EMC3 mutant homozygous cells. (F)  Expression of UAS-HA-Xport-A4L (anti-HA, in blue) is normal in EMC3e02662 homozygous mutant clones. The rhabdomeres are stained for actin (Phalloidin, in green). Scale bars represent 10 μm."
NCBI gene IDs:
"Flippase: 32615
EMC3: 34479
eyeless: 43812"
Figure legend: "(B-D) Histograms of  flow cytometry  data monitoring the fluorescence protein ratio in the indicated U2OS cell lines for each construct. \"EMC5KO+EV\" indicates a knockout of EMC5  stably harbouring empty vector, while ''EMC5KO+EMC5\" indicates EMC5KO cells rescued by inducible re-expression of a stably integrated EMC5. (E-G) Histograms of flow cytometry data monitoring the fluorescence protein ratio in the indicated U2OS  cell lines for each construct. \"EMC6KO+EV\" indicates a knockout of EMC6 stably harbouring empty vector, while ''EMC6KO+EMC6\" indicates EMC6KO cells rescued by re-expression of a stably integrated EMC6."
NCBI gene IDs:
"EMC6: 83460
EMC5: 93380"
Figure legend: "(C-G) (C-D, F) Western-Blot  of WT and EMC6KO (6KO)  U2OS cell lines transiently transfected with the opsin-tagged constructs probed for HA. When indicated, denatured samples were treated with  PNGase  to confirm glycosylation. Glycosylated proteins shift upwards in the gel and are indicated by arrows (). (E, G) Western-Blot of WT and EMC6KO (6KO)  U2OS  cell lines transiently transfected with the  opsin-tagged constructs probed for Actin  and EMC6."
NCBI gene IDs:
"opsin: 
EMC6: 83460"
Figure legend: "(A-B)  Immunostaining of  3rd instar larval eye imaginal discs with  eyeless-Flippase-induced clones of cells homozygous for  EMC3Δ4, labelled by the absence of ubiGFP (green) show loss of (A) UAS-Xport-A-HA (anti-HA, in blue) and UAS-Rh1 (4C5, in red). (B) Expression of UAS-Xport-A4L (anti-HA, in blue) and UAS-Rh1 (4C5, in red) is observed in  EMC3Δ4 homozygous cells. The UAS constructs were expressed under the control of GMR-GAL4. Scale bars represent 10 μm."
NCBI gene IDs:
"Flippase: 32615
EMC3: 34479
eyeless: 43812
GAL4: 855828"
Figure legend: "(C-D)  Immunostaining of mosaic adult retinas with  eyeless-Flippase-induced clones of cells homozygous for  EMC3e02662, labelled by the absence of RFP (red), show loss of (C) TRP (Mab83F6, in blue) when UAS-HA-Xport-A is expressed. (D)  Expression of TRP  (Mab83F6, in blue) is observed in EMC3e02662 homozygous cells, when HA-Xport-A4L is expressed. The rhabdomeres are stained  for actin  (Phalloidin, in green). The UAS constructs were expressed under the control of Rh1-GAL4. Scale bars represent 10 μm."
NCBI gene IDs:
"Flippase: 32615
EMC3: 34479
eyeless: 43812
GAL4: 855828
Rh1: 246602"
Figure legend: "(E-G) Western blot  of fly heads labelled for  V5, TRP,  Rh1, Xport-A (Xp-A) and tubulin. (E)  Immunoblot of fly heads expressing Rh1Gal4 (driver) (left) or Rh1 TMD1-V5 (right) in  Xport-A  heterozygous (Xport-A1/TM6B) or homozygous mutant flies (Xport-A1/Xport-A1). Extracts were untreated (-) or treated (+) with  PNGase F enzyme (lanes 2, 4, 6 and 8). For Rh1Gal4, 4 fly heads were loaded per well, while for  Rh1 TMD1-V5, 8  fly heads were loaded per well. (F) Immunoblot of  fly heads expressing Rh1 TMD1-3-V5 in Xport-A heterozygous (Xport-A1/TM6B) or homozygous mutant flies (Xport-A1/Xport-A1). Extracts were untreated (-) or treated (+) with PNGase F  enzyme (lanes 2 and 4). Eight fly heads  were loaded per lane. (G) Immunoblot of fly heads expressing Rh1 TMD1-5-V5 in Xport-A heterozygote (Xport-A1/TM6B) or homozygous mutant flies (Xport-A1/Xport-A1). Extracts were untreated (-) or treated (+) with either Endo-H  (lanes 2 and 5) or PNGase F enzyme (lanes 3 and 6). Each lane contains approximately 2,5 fly heads."
NCBI gene IDs:
"TM6B: 
TMD1: 
V5: 
Gal4: 855828
Rh1: 246602
Xport-A: 42373"
Figure legend: "(B) Autophagy‐negative phenotype of the apg16Δ strain. The autophagic ability of wild‐type (TN125), apg12Δ (YNM107) and apg16Δ cells (YNM114) was measured by the alkaline phosphatase assay before (black bars) and after (white bars) nitrogen starvation for 4 h. Error bars indicate the standard deviation of three independent experiments."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "(C) Loss of viability during starvation. Wild‐type (KA311B, ○), apg12Δ (YNM101, ●) and apg16Δ cells (YNM124, ▴) were cultured in nitrogen starvation medium, and their viability was determined by phloxine B staining."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "(D) Defect in API maturation in apg16Δ cells. Transport of pro‐API to the vacuole was examined by immunoblotting with anti‐API antiserum. The positions of precursor and mature API are indicated."
NCBI gene IDs:
"apg16: 855194"
Figure legend: "(E) The Apg12p-Apg5p conjugation in apg16Δ cells. Total lysates were prepared by the NaOH/2‐mercaptoethanol extraction method from apg12Δ (YNM107) and apg12Δapg16Δ cells (YNM115), both carrying pHA‐APG12 (CEN). Western blot analysis was performed using anti‐HA antibody."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "Apg16p interacts with the Apg12p-Apg5p conjugate but not with the Apg12p monomer. The apg12Δapg16Δ cells (YNM115) were transformed with high copy (2μ) plasmids encoding MycApg12p and/or HAApg16p as indicated. Total lysates were immunoprecipitated with anti‐Myc (lanes 1-4) or anti‐HA antibody (lanes 5-8) and detected by immunoblotting using anti‐Myc or anti‐HA antibody. The positions of the IgG heavy chain and κ light chain are indicated."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "Apg16p interacts preferentially with Apg12p‐modified Apg5p. Total lysates were prepared from apg16Δ cells (YNM124) or apg12Δapg16Δ cells (YNM115) harboring both pHA‐APG5 and pMyc‐APG16 CEN plasmids. The lysates were immunoprecipitated and analyzed as described in Figure 3."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "Apg16p interacts preferentially with Apg12p‐modified Apg5p. Total lysates were prepared from apg16Δcells (YNM124) or apg12Δapg16Δ cells (YNM115) harboring both pHA‐APG5 and pMyc‐APG16 CEN plasmids. The lysates were immunoprecipitated and analyzed as described in Figure 3."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "(A) PJ69‐4A cells were co‐transformed with each pGBD and pGAD plasmid as indicated. Transformants were selected on Trp− Leu− plates, and then two‐hybrid interaction (+ or −) was assessed for growth on Ade− Trp− Leu− plates."
NCBI gene IDs:
"pGAD: 
pGBD: "
Figure legend: "(B) Self‐multimerization of Apg16p in vivo. Lysates of apg16Δ cells (YNM124) transformed with pMyc‐APG16 (2μ) and/or pHA‐APG16 (2μ) were immunoprecipitated with anti‐Myc antibody and detected with anti‐HA antibody."
NCBI gene IDs:
"apg16: 855194"
Figure legend: "Apg16p mediates Apg5p assembly. Immunoprecipitation was carried out as described in Figure 3, using apg12Δ (YNM107) and apg12Δapg16Δ cells (YNM115) carrying pMyc‐APG12 (CEN) and pHA‐APG12 (CEN) (A), or pMyc‐APG12 (CEN) and pHA‐APG5 (CEN) (B). Following SDS-PAGE, immunoprecipitates were subjected to immunoblot analysis using anti‐HA or anti‐Myc antibody as indicated. A model of the Apg12p-Apg5p-Apg16p complex is illustrated in each panel. Apg16p forms a homo‐oligomer through its C‐terminal coiled‐coil region. It is not clear whether Apg16p forms a dimer or a larger multimer. The N‐terminal region of each Apg16p interacts with Apg5p; most of these complexes are conjugated to Apg12p (A) but a small population are unmodified (B). In the absence of Apg16p, separate Apg5ps do not assemble."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "Apg16p mediates Apg5p assembly. Immunoprecipitation was carried out as described in Figure 3, using apg12Δ (YNM107) and apg12Δapg16Δ cells (YNM115) carrying pMyc‐APG12 (CEN) and pHA‐APG5 (CEN) (B). Following SDS-PAGE, immunoprecipitates were subjected to immunoblot analysis using anti‐HA or anti‐Myc antibody as indicated. A model of the Apg12p-Apg5p-Apg16p complex is illustrated in each panel. Apg16p forms a homo‐oligomer through its C‐terminal coiled‐coil region. It is not clear whether Apg16p forms a dimer or a larger multimer. The N‐terminal region of each Apg16p interacts with Apg5p; most of these complexes are conjugated to Apg12p (A) but a small population are unmodified (B). In the absence of Apg16p, separate Apg5ps do not assemble."
NCBI gene IDs:
"apg12: 852518
apg16: 855194"
Figure legend: "Expression levels of Apg16p are dependent on the Apg12p-Apg5p conjugate. Total lysates were prepared by the NaOH/2‐mercaptoethanol extraction method from apg16Δ (YNM124), apg5Δapg16Δ (YNM126) and apg12Δapg16Δ cells (YNM115) carrying pHA‐APG16 (CEN). Western blot analysis was performed using anti‐HA antibody."
NCBI gene IDs:
"apg12: 852518
apg16: 855194
apg5: 855954"
Figure legend: "Apg16p exists in a pellet fraction in an Apg5p‐dependent manner. Spheroplasts were generated from cells harboring pHA‐APG5 (A), pHA‐APG16 (B) or pHA‐APG16 (2μ) with or without pHA‐APG5 (2μ) (C). They were lysed by Dounce homogenization. After unbroken spheroplasts were removed by low‐speed centrifugation, the supernatant (T) was fractionated by 100 000 g centrifugation for 60 min to generate a supernatant (S) and pellet (P) fraction. Each fraction was subjected to immunoblot analysis using anti‐HA antibody."
NCBI gene IDs:
"APG16: 855194
APG5: 855954"
Figure legend: "(A) IDH1 acetylation levels upon treatment with NAM or TSA. Flag-tagged IDH1 was ectopically expressed in HEK293T cells treated with NAM (5 mM) and/or TSA (0.5 mM) for the indicated time period."
NCBI gene IDs:
"Flag: 
IDH1: 3417"
Figure legend: "(C) Mutation of IDH1 K224R and K224Q resulted in altered acetylation levels using Western Blot."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(D) IDH1 catalytic activity of IDH1 K224 mutants in vitro. Wild-type IDH1 and K224R, K224Q mutants were expressed in HEK293 cells. Proteins were purified by immunoprecipitation (IP), IDH1 levels were normalized for protein, and activity assays were performed. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For multiple comparisons in (D), a one-way ANOVA with Newman-Keuls post hoc test was used to test for statistical significance."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(E-F) Steady-state kinetic analysis of IDH1 and variants in HEK293T cells. Comparison of IDH1 WT and K224R and K224Q showed that the K224 site was important in catalysis. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For (E-F), statistical significance was determined using the two-way ANOVA followed by Tukey's post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(G) IDH1 directly interacted with SIRT2 but not SIRT1. SIRT2 deacetylated IDH1 effectively. Flag tagged IDH1 and HA tagged SIRT1 or SIRT2 were co-transfected into cells. The acetylation level of Flag-bead-purified IDH1 and the protein association between IDH1 and SIRT1 or SIRT2 were determined by Western blot."
NCBI gene IDs:
"Flag: 
HA: 
IDH1: 3417
SIRT1: 23411
SIRT2: 22933"
Figure legend: "(K) IDH1 K224 deacetylation was dependent on SIRT2 catalytic activity. SIRT2 H187Y was a catalytically inactive mutant."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(A) Colony formation ability after transfection of SIRT2 plasmids, AGK2 and SIRT2 siRNA. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For A statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(B) Transwell assay was performed to evaluate the invasion abilities of HCT116 cells after overexpression or downregulation of SIRT2 or treated with AGK2. Stained cells in the lower chambers were quantified. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(D) Cell cycle distribution was evaluated flow cytometrically after treatment with SIRT2 plasmids, SIRT2 siRNA or AGK2. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For D, statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"SIRT2: 22933"
Figure legend: "(A-B) IDH1 K224 acetylation level was enhanced with increasing glucose concentration. Flag-IDH1 was overexpressed in cells treated with increased concentrations of glucose for 6 h. IDH1 proteins were purified by Flag beads, and then IDH1 K224 acetylation level was determined by Western blot and IDH1 catalytic activity was assessed. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For B statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"Flag: 
IDH1: 3417"
Figure legend: "(C-D) IDH1 K224 acetylation level was upregulated and IDH1 activity was weakened with increasing glutamine concentration. Flag-IDH1 was overexpressed in cells treated with increased glutamine for 6 h. IDH1 proteins were purified by Flag beads, and the K224 acetylation level of IDH1 was determined by Western blot analysis and then IDH1 catalytic activity was evaluated. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For , D statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"Flag: 
IDH1: 3417"
Figure legend: "Downregulation of SIRT2 diminished the effect of glucose on changing IDH1 K224 acetylation. Flag-tagged IDH1 was overexpressed in HCT116 cells with or without transient SIRT2 knockdown. The cells were treated with different concentrations of glucose (E)"
NCBI gene IDs:
"Flag: 
IDH1: 3417
SIRT2: 22933"
Figure legend: "Downregulation of SIRT2 diminished the effect of glutamine on changing IDH1 K224 acetylation. Flag-tagged IDH1 was overexpressed in HCT116 cells with or without transient SIRT2 knockdown. The cells were treated with different concentrations of glutamine (F)."
NCBI gene IDs:
"Flag: 
IDH1: 3417
SIRT2: 22933"
Figure legend: "(G) IDH1 K224 deacetylation regulated cellular NADPH/NADP+ redox in cells. In HCT116 stable cells with IDH1 knockout and re-expressing the indicated proteins, the ratio of NADPH/NADP+ in cells was measured as followed by manufacture's instruction. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For , G statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(H) IDH1 K224 deacetylation promoted GSH production in HCT116 cells. In HCT116 stable cells with IDH1 knockout and re-expressing the indicated proteins, the ratio of GSH/GSSG was assessed as followed by manufacture's instruction. 1 Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For H, statistical significance was assessed with the one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(I) IDH1 K224 deacetylation suppressed cellular ROS levels in HCT116 cells. ROS was determined in cells under non-stressed condition or exposed to menadione. *P&lt;0.05 and **P&lt;0.01 Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For figure I, statistical significance was assessed with the two-way ANOVA followed by Tukey's post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(A) Gene expression profile array analysis in HCT116 cells. Representative genes with higher fold difference (up or down) compared IDH1 K224R cells with IDH1 WT cells. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition)."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(B) ChIP assays for HIF1α and its binding motif. Antibodies anti-IgG and anti- HIF1α were used in the ChIP assays. qRT-PCR was performed to quantify the binding activity. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For B , statistical significance was assessed with a two-tailed unpaired Student's t-test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"HIF1α: 3091"
Figure legend: "(C-D) The transcriptional activity of the SRC promoter was greatly increased after co-expression of HIF1α and SRC reporter in wild type. SRC promoter constructs containing a potential HIF1α binding motif (-1636~-1629 bp and -1466~-1459 bp). Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For D, statistical significance was assessed with a two-tailed unpaired Student's t-test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"HIF1α: 3091
SRC: 6714"
Figure legend: "(E) IDH1 K224 deacetylation led to degradation of HIF1α but upregulation of hydroxy-HIF1α. In HCT116 stable cells with IDH1 knockout and re-expressing the indicated proteins, HIF1α and hydroxy-HIF1α was measured by Western blot assay."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(F) HCT116 cells of IDH1 K224Q were cotreated with Octyl-α-KG and NAC, the expression of HIF1α and SRC was further evaluated."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "HCT116 cells expression IDH1 K224Q with or without treatment with Octyl-α-KG and/or NAC were analyzed for glucose levels. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For G statistical significance was assessed with a one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "HCT116 cells expression IDH1 K224Q with or without treatment with Octyl-α-KG and/or NAC were analyzed for lactate levels. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For H, statistical significance was assessed with a one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01. n.s. = not significant."
NCBI gene IDs:
"IDH1: 3417"
Figure legend: "(A) Transwell assay was performed to evaluate the invasion abilities of HCT116 cells after transfection of IDH1 K224R or knockout of IDH1. Quantification was performed by counting the stained cells that invaded to the lower chamber under a light microscopy. Data information: All results were expressed as mean ± SD of three independent experiments (n ≥ 3 per experimental condition). For A statistical significance was assessed with a one-way ANOVA with Newman-Keuls post hoc test. *P&lt;0.05 and **P&lt;0.01."
NCBI gene IDs:
"IDH1: 3417"