EMBO/SourceData huggingface dataset:
Figure legend: "Body weights of WT and Rcan1-KO mice fed normal chow diet (NC) for 20 weeks starting at 8-10 weeks of age (male, n=15 each). Data information: values shown are mean ±SD * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001 (two-way ANOVA with multiple comparisons, except for A and B, which used multiple t-tests)."
NCBI gene IDs:
"Rcan1: 54720"
Figure legend: "Body weights of WT and Rcan1-KO mice fed a high-fat diet (HFD) for 20 weeks starting at 8-10 weeks of age (male, n=15 each). Data information: values shown are mean ±SD * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001 (two-way ANOVA with multiple comparisons, except for A and B, which used multiple t-tests)."
NCBI gene IDs:
"Rcan1: 54720"
Figure legend: "Cholesterol remaining in the feces of WT and Rcan1-KO mice fed a HFD, measured as mMol per mg of feces (males, n=4 over 3 days). Cholesterol content of NC and the HFD chow (input) were measured for comparison. Data information: values shown are mean ±SD * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001 (two-way ANOVA with multiple comparisons, except for A and B, which used multiple t-tests)."
NCBI gene IDs:
"Rcan1: 54720"
Figure legend: "triglyceride remaining in the feces of WT and Rcan1-KO mice fed a HFD, measured as mMol per mg of feces (males, n=4 over 3 days). triglyceride content of NC and the HFD chow (input) were measured for comparison. Data information: values shown are mean ±SD * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001 (two-way ANOVA with multiple comparisons, except for A and B, which used multiple t-tests)."
NCBI gene IDs:
"Rcan1: 54720"
Figure legend: "L Transcript levels for inflammatory markers Monocyte chemoattractant protein-1 (Mcp-1), macrophage antigen F4/80 (F480), tumor necrosis factor-alpha (Tnfα), and tumor necrosis factor-beta (Tnfβ) in gWAT of WT and KO after 25 weeks on NC or HFD (males, n=4-5). Transcript levels were normalized to 18S Data information: values shown are mean ±SD * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001 (two-way ANOVA with multiple comparisons, except for A and B, which used multiple t-tests)."
NCBI gene IDs:
"F480: 13733
macrophage antigen F4/80 : 13733
Mcp-1: 20296
Monocyte chemoattractant protein-1: 20296
Tnfβ: 16992
tumor necrosis factor-beta: 16992
tumor necrosis factor-alpha: 21926"
Figure legend: "C Transcript levels for genes involved in lipid metabolism and gluconeogenesis in livers of WT and KO mice after 25 weeks on NC or HFD. Stearoyl-Coenzyme A desaturase (Scd1), fatty acid transporter (CD36), glycerol-3-phosphate acyltransferase (Gpat), carnitine palmitoyltransferase I (Cpt1), peroxisome proliferator-activated gamma (PPARγ), pyruvate dehydrogenase acetyl-transferase (Pdk4), PPARγ coactivator 1 alpha (PGC1α), diacylglycerol O-acyltransferase (Dgat), and facilitated glucose transporter (Glut4). (males, n=4-5). Transcript levels were normalized to 18S. Data information: values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01; *** = p &lt; 0.001; **** = p &lt; 0.0001 (two-way ANOVA with multiple comparisons)."
NCBI gene IDs:
"18S: 
CD36: 12491
fatty acid transporter: 12491
carnitine palmitoyltransferase I: 12895///12894
Cpt1: 12895///12894
Dgat: 13350
diacylglycerol O-acyltransferase: 13350
glycerol-3-phosphate acyltransferase: 14732
Gpat: 14732
Pdk4: 27273
pyruvate dehydrogenase acetyl-transferase: 27273
peroxisome proliferator-activated gamma: 19016
PPARγ: 19016
PGC1α: 19017
PPARγ coactivator 1 alpha: 19017
Scd1: 20249
Stearoyl-Coenzyme A desaturase: 20249
glucose transporter: 20528
Glut4: 20528"
Figure legend: "Transcript levels for Rcan1.1 and Rcan1.4 during differentiation of 3T3-L1 adipocytes, measured at 0, 1, 2, 3, 4, 6 and 24 hours following the addition MDI as well as at 3, 7 and 10 days as indicated (n=3). * = p &lt; 0.05; ** = p &lt; 0.01, student's t-test, unpaired, vs 0 time point. Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD"
NCBI gene IDs:
"18S: 
Rcan1.1: 54720
Rcan1.4: 54720"
Figure legend: "B Western blot for RCAN1.1, RCAN1.4 and tubulin (TUB) in whole cell extracts of 3T3-L1 adipocytes transfected with a control si-RNA or ones targeting either Rcan1.4 or Rcan1.1 (20 μg per lane)."
NCBI gene IDs:
"Rcan1.1: 54720
Rcan1.4: 54720"
Figure legend: "E Transcript levels for Pgc1-α, in 3T3-L1 adipocytes transfected with the indicated si-RNAs and assayed over 10-days of differentiation (n=3). Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD"
NCBI gene IDs:
"18S: 
Pgc1-α: 19017"
Figure legend: "G Transcript levels for Adrenoceptor beta 3 (Adrb3), uncoupling protein 1 (Ucp1), PPARγ coactivator 1 alpha (PGC1α), and deiodinase type II (Dio2) Ing-svf cultures transfected with control siRNA (C) or one targeting Rcan1 (KD) then maintained in growth media (G), or differentiated for ten-days (D) with and without the addition of 100 mM isoproterionol for 6 hours prior to harvesting (D+ISO) (n=2 in triplicate). * = p &lt; 0.05; ** = p &lt; 0.01, two-way ANOVA with multiple comparisons. Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD"
NCBI gene IDs:
"18S: 
Adrb3: 11556
Adrenoceptor beta 3: 11556
deiodinase type II: 13371
Dio2: 13371
PGC1α: 19017
PPARγ coactivator 1 alpha: 19017
Rcan1: 54720
Ucp1: 22227
uncoupling protein 1: 22227"
Figure legend: "H, I Transcript levels for the indicated panel of genes relevant to adipocyte differentiation in Platelet-derived growth factor receptor alpha-positive (PDGFRα+) and lineage-negative (Lin-) stromal vascular cells isolated by FACS sorting from sWAT of WT (black bars) and KO (red bars). In H, isolated cells were maintained in growth media. In I, cultures were treated with MDI then differentiated for 10 days. * = p &lt; 0.05; ** = p &lt; 0.01, student's t-test, unpaired, WT vs. KO. Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD"
NCBI gene IDs:
"18S: 
PDGFRα: 18590
Platelet-derived growth factor receptor alpha: 18595"
Figure legend: "Transcript levels of Pgc1α and Ucp1 in sWAT of male and female WT and KO mice after 24 hours at RT or 4°C (n= Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01. (two-way ANOVA with multiple comparisons"
NCBI gene IDs:
"18S: 
Pgc1α: 19017
Ucp1: 22227"
Figure legend: "C Transcript levels of Ucp1, Pgc1α, Adrb3, retinoblastoma 1 (Rb1), zinc finger protein 423 (Zfp423), and PR domain containing 16 (Prdm16) were quantified in sWAT of WT and KO mice after 25 weeks on a HFD (n=4-5). Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01. ; unpaired student's t-test"
NCBI gene IDs:
"18S: 
Adrb3: 11556
Pgc1α: 19017
PR domain containing 16: 70673
Prdm16: 70673
Rb1: 19645
retinoblastoma 1: 19645
Ucp1: 22227
Zfp423: 94187
zinc finger protein 423: 94187"
Figure legend: "D Transcript levels of Pgc1α-1b and Pgc1α-1a isoforms in sWAT of WT and KO 8 week old females maintained at 24°C (RT) or housed at 4°C for 24 hours (n=5). Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01. (two-way ANOVA with multiple comparisons"
NCBI gene IDs:
"18S: 
Pgc1α-1a: 19017
Pgc1α-1b: 19017"
Figure legend: "E. Western blot for RCAN1 in various skeletal muscle groups. Each lane was loaded with 20 μg of total protein from plantaris (PL), soleus (SOL), extensor digitorum longus (EDL), tibialis anterior (TA), red gastrocnemius (RG), white gastrocnemius (WG), white vastus lateralis (WV), heart ventricle (HT), atria, diaphragm (Dia), and Tong. The lane containing brain extract was loaded with 10 μg of protein. Antibody specificity was validated by running 20 μg of protein from the heart of an Rcan1-KO animal (HT-KO). The asterisks * indicate the location of putative proteolytic fragments of RCAN1.1."
NCBI gene IDs:
"Rcan1: 54720"
Figure legend: "F Transcript levels of Sln in skeletal muscle (tibialis anterior) of 8-10 week old WT and KO mice housed at RT. (n=4-5 each gender). G Transcript levels of Sln in skeletal muscle (tibialis anterior ) of 8-10 week old male WT and KO animals housed at RT. (n=5).Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01. (two-way ANOVA with multiple comparisons F, ; unpaired student's t-test in G."
NCBI gene IDs:
"18S: 
Sln: 66402"
Figure legend: "J Activity of the Sln-Luc reporter in C2C12 myoblasts transiently transfected with an empty control vector or ones expressing constitutively active calcineurin (CnA*) and Rcan1. Luciferase activity was normalized to beta-galactosidase activity from a co-transfected vector. (n=3, assayed in duplicate). Data information: Transcript levels were normalized to 18S. All values shown are mean ±SD. * = p &lt; 0.05; ** = p &lt; 0.01. (two-way ANOVA with multiple comparisons"
NCBI gene IDs:
"18S: 
beta-galactosidase: 
Luc: 
Luciferase: 
calcineurin: 19055
CnA: 19055
Rcan1: 54720
Sln: 66402"
Figure legend: "B, C Correlation of Rcan1 expression in gonadal adipose and liver with body weight, plasma insulin and plasma triglycerides, in a data set comparing backgrounds susceptible (BTBR background) or resistant (C57BL/6 background) to diabetes when carrying the leptinob/ob (ob) mutation. Measures are reported as the ratio of the mean log10 intensity (ml ratio). Regression line (black), r = linear regression, p = p-value."
NCBI gene IDs:
"leptin: 16846"
Figure legend: "(C-D) RNA-Seq data from GTEx of the normalized expression of human CSAG1 (C) and CSAG2 (D) in the indicated tissues. Number of biological replicates are as follows: testis 361, brain 255, small intestine 187, white adipose 663, muscle 803, skin 604, esophagus 555, stomach 359, liver 226, heart 429. blood vessel 663, lung 578, colon 373, nerve 619, pituitary 283, blood 755, adrenal gland 258, kidney 85, prostate 245, salivary gland 162, ovary 180, breast 459, pancreas 328, vagina 156, uterus 142, spleen 241, fallopian tube 9, bladder 21, cervix 10. Central band indicates median, boxes define 25 and 75 percentiles, and whiskers define 5 and 95 percentiles."
NCBI gene IDs:
"CSAG1: 158511
CSAG2: 102723547"
Figure legend: "(E-F) Expression of CSAG1 (E) and CSAG2 (F) in normal adjacent (blue) or tumor tissue (red) from TCGA RNA-Seq data. N.D. represents normal adjacent is not available. Data visualized by firebrowse. Number of biological replicates are as follows: ACC 41, BLCA 307, BRCA 524, CESC 175, CHOL 11, COAD 153, COADREAD 232, DLBC 36, ESCA 121, HNSC 416, KICH 46, KIPAN 515, KIRC 411, KIRP 58, LAML 7, LGG 528, LUAD 301, LUSC 400, MESO 31, OV 183, PCPG 70, PRAD 204, READ 79, SARC 156, SKCM 422, STAD 288, STES 409, TGCT 126, THCA 408, THYM 75, UCEC 306, and UCS 37. Central band indicates median, boxes define 25 and 75 percentiles, and whiskers define 5 and 95 percentiles."
NCBI gene IDs:
"CSAG1: 158511
CSAG2: 102723547"
Figure legend: "(H-K) Expression of CSAG1 (H-I) or CSAG2 (J-K) in patients from indicated cancer types correlates with poor overall survival. Data is from TCGA and visualized by kmplotter."
NCBI gene IDs:
"CSAG1: 158511
CSAG2: 102723547"
Figure legend: "(A-B) The effect of stable knockdown CSAG2 in HCT116 (A) or A375 (B) were assayed for clonogenic growth on plastic for 7 days. The number of colonies was quantified and is shown. Data are represented as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(C-D) The effect of stable knockdown CSAG2 in HCT116 (C) or A375 (D) were determined for anchorage-independent growth in soft agar colony formation assays for 14 days. The number of colonies was quantified and is shown. Data are represented as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(E-F) Knockdown of CSAG2 in A375 (F) or HCT116 (E) cells decreases xenograft tumor growth in mice. Cells with doxycycline-inducible shRNAs were implanted into NOD SCID gamma mice. Doxycycline (2 mg/mL) was administered via drinking water. Tumor growth was monitored over time. Data are represented as the mean ± SD, n=6 mice for each group."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(G-H) Stable expression of CSAG2 in HCT116 (G) and CSAG2-negative H460 (H) cells increases anchorage-independent growth in soft agar colony formation assays, n=3 biological replicates. Data are represented as the mean ± SD."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(A) HEK293 cells stably expressing TAP-vector, TAP-CSAG1, or TAP-CSAG2 were subjected to pull-down followed by SDS-PAGE and LC-MS/MS. Spectral counts (SC) of the identified proteins in TAP-CSAG1 and TAP-CSAG2 pulldowns, but absence in TAP-vector control, are shown."
NCBI gene IDs:
"CSAG1: 158511
CSAG2: 102723547"
Figure legend: "(B) HEK293FT cells stably expressing HA-SIRT1 were transfected with Myc-CSAG1 or Myc-CSAG2 for 48 h before IP with anti-HA followed by SDS-PAGE and immunoblotting for indicated proteins."
NCBI gene IDs:
"HA: 
Myc: 
CSAG1: 158511
CSAG2: 102723547
SIRT1: 23411"
Figure legend: "Expression of CSAG2 decreased p53 ac-K382 levels and downregulated the expression of downstream p53 target genes in H460 cells. Cells were treated by either 1 μM doxorubicin (D-E) with 0.4 μM TSA for the indicated times. Cells were then harvested and blotted for the indicated proteins. Quantitation of expression levels is shown (E Ac-p53 K382 levels normalized to total p53, whereas all other proteins were normalized to GAPDH."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Expression of CSAG2 decreased p53 ac-K382 levels and downregulated the expression of downstream p53 target genes in H460 cells. Cells were treated by 20 μM etoposide with 0.4 μM TSA for the indicated times. Ac-p53 K382 levels normalized to total p53, whereas all other proteins were normalized to GAPDH."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Expression of CSAG2 decreased p53 ac-K382 levels and downregulated the expression of downstream p53 target genes in H460 cells. Cells were treated by 20 μM etoposide with 0.4 μM TSA for the indicated times. Cells were then harvested and blotted for the indicated proteins. Quantitation of expression levels is shown G) as the mean ± SD, n=3 biological replicates. Ac-p53 K382 levels normalized to total p53, whereas all other proteins were normalized to GAPDH."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Knockdown of endogenous CSAG2 in HCT116 (H-I) cells increased p53 ac-K382 levels. CSAG2-knockdown stable cells were treated by 1 μM doxorubicin/ 0.4 μM TSA for 6 hr. Cells were then harvested and blotted for the indicated proteins. Quantitation of ac-p53 K382 levels relative to total p53 is shown (I as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Knockdown of endogenous CSAG2 in A375 cells increased p53 ac-K382 levels. CSAG2-knockdown stable cells were treated by 1 μM doxorubicin/ 0.4 μM TSA for 6 hr. Cells were then harvested and blotted for the indicated proteins."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Knockdown of endogenous CSAG2 in A375 cells increased p53 ac-K382 levels. CSAG2-knockdown stable cells were treated by 1 μM doxorubicin/ 0.4 μM TSA for 6 hr. Cells were then harvested and blotted for the indicated proteins. Quantitation of ac-p53 K382 levels relative to total p53 is shown K) as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(L-M) Re-expression of CSAG2 rescued p53 ac-K382 levels in CSAG2 knockdown stable cell lines. Myc-CSAG2 was transfected in HCT116 (L) or A375 (M) CSAG2 knockdown stable cell lines. Cells were treated with 1 μM doxorubicin/ 0.4 μM TSA for 6 hr and then blotted for the indicated proteins. Quantitation of ac-p53 K382 levels relative to total p53 is shown as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547"
Figure legend: "(N) CSAG2 does not enhance anchorage-independent growth of HCT116 p53-null cells. CSAG2 was stably expressed in isogenic HCT116 p53-wild-type or p53-null cells. Anchorage-independent growth in soft agar colony formation assays was determined after 14 days. The number of colonies was quantified, normalized to each vector control cell line, and is shown as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547
p53: 7157"
Figure legend: "(A) Knockdown of SIRT1 abolished CSAG2-mediated decrease in ac-p53 K382. CSAG2 over-expressing HCT116 were transfected with SIRT1 siRNA for 72 hr before doxorubicin/TSA treatment for 6 hr. Cell lysates were blotted for the indicated proteins."
NCBI gene IDs:
"CSAG2: 102723547
SIRT1: 23411"
Figure legend: "(B) Inhibition of SIRT1 enzymatic activity blocked CSAG2-induced decrease in p53 ac-K382. CSAG2 over-expressing HCT116 cells were treated with 1 μM doxorubicin/ 0.4 μM TSA with or without 1 μM EX-527 for 6 hr. Cell lysates were blotted for the indicated proteins."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Inhibition of SIRT1 enzymatic activity prevents CSAG2-knockdown increase in p53 ac-K382. CSAG2-knockdown stable cells were treated by 1 μM doxorubicin/ 0.4 μM TSA with or without 1 μM EX-527 for 6 hr. Cells were then harvested and blotted for the indicated proteins (C)"
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Inhibition of SIRT1 enzymatic activity prevents CSAG2-knockdown increase in p53 ac-K382. CSAG2-knockdown stable cells were treated by 1 μM doxorubicin/ 0.4 μM TSA with or without 1 μM EX-527 for 6 hr. Cells were then harvested and blotted for the indicated proteins and quantitated (D; n=3 biological replicates). Data shown as mean ± SD."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(G) Inhibition of SIRT1 activity abolished CSAG2-induced anchorage-independent growth. Soft agar colony formation assays were performed in control or CSAG2 over-expressing HCT116 cells with or without EX-527 treatment. Data are represented as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(H) Summary of region on CSAG2 required for SIRT1 interaction. HEK293FT cells stably expressing HA-SIRT1 were transfected with indicated CSAG2 constructs for 48 h before IP with anti-HA followed by SDS-PAGE and immunoblotting for anti-Myc."
NCBI gene IDs:
"HA: 
CSAG2: 102723547
SIRT1: 23411"
Figure legend: "(J) CSAG2 Δ37 mutant does not regulate p53 ac-K382 levels. Cells were transfected with the indicated Myc-CSAG2 constructs for 48 hr before being treated with 1 μM doxorubicin/ 0.4 μM TSA. Cell lysates were blotted for the indicated proteins."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547"
Figure legend: "(K) CSAG2 Δ37 fails to promote anchorage-independent growth of H460 cells. Soft agar colony formation assays were performed in CSAG2-negative H460 cells stably expressing CSAG2 wild-type or CSAG2 Δ37 mutant (n=3 biological replicates). Data are represented as the mean ± SD."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Control or CSAG2 expressing H460 cells were treated with the indicated concentrations of doxorubicin (A) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Control or CSAG2 expressing H460 cells were treated with the indicated concentrations of H2O2 (B) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "shCTRL, shCSAG2-1, or shCSAG2-2 stable HCT116 cells were treated with the indicated concentrations of doxorubicin (C) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "shCTRL, shCSAG2-1, or shCSAG2-2 stable HCT116 cells were treated with the indicated concentrations of H2O2 (D) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(G) H460 cells were transfected with wild type, Δ37, or Δ63 CSAG2 for 48 hr. Cells were then treated with the indicated concentrations of H2O2 for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "(H) Inhibition of SIRT1 activity abolished CSAG2 induced cell survival under genotoxic stress. CSAG2 over-expressing H460 cells were treated with the indicated concentrations of H2O2 with or without 1 μM EX-527 for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547"
Figure legend: "Control or Myc-CSAG2 expressing HCT116 p53 -/- cells were treated with the indicated concentrations of H2O2 (I) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547
p53: 7157"
Figure legend: "Control or Myc-CSAG2 expressing HCT116 p53 -/- cells were treated with the indicated concentrations of doxorubicin (J) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547
p53: 7157"
Figure legend: "shCTRL, shCSAG2-1, or shCSAG2-2 stable HCT116 p53 -/- cells were treated with the indicated concentrations of H2O2 (K) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547
p53: 7157"
Figure legend: "shCTRL, shCSAG2-1, or shCSAG2-2 stable HCT116 p53 -/- cells were treated with the indicated concentrations of doxorubicin (L) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"CSAG2: 102723547
p53: 7157"
Figure legend: "Control or Myc-CSAG2 expressing H1299 (p53 mutant) cells were treated with the indicated concentrations of H2O2 (M) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547
p53: 7157"
Figure legend: "Control or Myc-CSAG2 expressing H1299 (p53 mutant) cells were treated with the indicated concentrations of doxorubicin (N) for 24 hr before cell viability was determined by alamarBlue (n=6 biological replicates)."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547
p53: 7157"
Figure legend: "(A) CSAG2 does not alter SIRT1 protein levels. HEK293FT cells were transfected with different amounts of Myc-CSAG2 for 48 hr before cells lysates were harvested and immunoblotted for indicated proteins."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547"
Figure legend: "(B) CSAG2 does not alter SIRT1 subcellular location. U2OS cells were transfected with or without mcherry-CSAG2 for 48 hr before fixation, SIRT1 immunostaining, and imaging. Scale bar: 10 µm."
NCBI gene IDs:
"mcherry: 
CSAG2: 102723547"
Figure legend: "(C) Summary of results mapping interaction region of SIRT1 recognized by CSAG2. HEK293FT cells stably expressing HA-CSAG2 were transfected with indicated Myc-SIRT1 constructs for 48 h before IP with anti-HA followed by SDS-PAGE and immunoblotting for anti-Myc."
NCBI gene IDs:
"HA: 
Myc: 
CSAG2: 102723547
SIRT1: 23411"
Figure legend: "(H-I) U2OS cells were transfected with Myc-Vector or Myc-CSAG2 for 48 hr before being treated with or without 0.4 μM TSA for 6 hr. Cell lysates were blotted for the indicated proteins (H). Quantitation of expression levels of acetylated Histone relative to total Histone is shown as the mean ± SD, n=3 biological replicates. White circles indicate Myc-Vector and black circles indicate Myc-CSAG2."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547"
Figure legend: "(J) HeLa, U2OS, or HCT116 cells were transfected with Myc-Vector or Myc-CSAG2 for 48 hr. Cell lysates were blotted for the indicated proteins. Quantitation of expression levels of acetylated Histone relative to total Histone is shown as the mean ± SD, n=3 biological replicates. White circles indicate Myc-Vector and black circles indicate Myc-CSAG2."
NCBI gene IDs:
"Myc: 
CSAG2: 102723547"
Figure legend: "(K) Knockdown of endogenous CSAG2 in A375 cells increased ac-H4K16 levels. Cell lysates were blotted for the indicated proteins. Quantitation of expression levels of ac-H4K16 relative to total H4 is shown as the mean ± SD, n=3 biological replicates."
NCBI gene IDs:
"CSAG2: 102723547"