EMBO/SourceData huggingface dataset:
Figure legend: "(A, B) SEPARASE+/+ and SEPARASE+/- MEFs were constitutively (− washout) or transiently exposed to DRB (+ washout) and then analysed by IF for cells with γH2AX- and 53BP1-positive foci. Shown are averages (bars) of 3 independent experiments (dots) counting ≥ 100 cells each. Scale bar = 5 μm. (C) Cell lysates from one experiment in (A) were analysed by immunoblotting using the indicated antibodies "
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: "SEPARASE+/+ and SEPARASE+/- MEFs were treated with DRB (+) or carrier solvent (−) for the indicated times and then analysed by immunoblotting of total cell lysates (D"
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: "SEPARASE+/+ and SEPARASE+/- MEFs were treated with DRB (+) or carrier solvent (−) for the indicated times and then analysed b IPs using anti-Rad21 or unspecific IgG (E). The lower right two lanes show in vitro-expressed mouse Rad21 treated with hyperactive (SA) separase or a protease-dead (PD) variant"
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: "(A) Transformation susceptibility of primary MEFs. SEPARASE+/+ versus SEPARASE+/- MEFs were infected to express E1A and hRasV12 and then subjected to a colony formation assay. Shown are representative images (left) and the graphical representation of the number of transformed foci (right) from two independent experiments (bars = standard deviations)"
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: " (B) Two-stage DMBA (initiation) plus TPA (promotion) skin carcinogenesis. The number and size of skin papillomas are plotted against time for each genotype: SEPARASE+/+ (n=8) versus SEPARASE+/- (n= 11) "
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: " (C) Representative hematoxylin- and eosin-stained sections from SEPARASE+/- biopsies. (i) glandular hyperplasia; (ii) benign papilloma; (iii) squamous cell carcinoma. Scale bars are 200 µm in (i) and 500 µm in (ii) and (iii). (D) Quantitative histological assessment of the DMBA-TPA skin cancerogenesis assay. Given are numbers of total, and corresponding percentage in brackets "
NCBI gene IDs:
"SEPARASE: 105988"
Figure legend: "A) Murine small intestines immunostained for the proliferation marker Ki67, apoptosis marker cleaved caspase 3 (Cas3), or YAP from 4 control (Yapfl/fl Tazfl/fl) and 4 YAP/TAZ double conditional knockout (Villin-CreERt Yapfl/fl Tazfl/fl) animals treated with tamoxifen. Tissues were harvested and analyzed at day 7 after first tamoxifen injection. Loss of YAP/TAZ leads to a moderate increase in apoptosis (Cas3+) in crypt base stem cells, quantified on the right, but no overall effect on the morphology of the small intestine. Arrows point to apoptotic cells. B) Murine large intestines immunostained for proliferation marker Ki67, apoptosis marker cleaved caspase 3 (Cas3), or YAP from control (Yapfl/fl Tazfl/fl) and YAP/TAZ double conditional knockout (Villin-CreERt Yapfl/fl Tazfl/fl) animals treated with tamoxifen. Tissues were harvested and analyzed at day 7 after first tamoxifen injection. Loss of YAP/TAZ leads to an increased number of apoptotic (Cas3+) cells within the transverse folds of the ascending colon."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
Taz: 66826
TAZ: 66826
Villin: 22349
Yap: 22601
YAP: 22601"
Figure legend: "C) Murine small intestines immunostained for YAP or cleaved caspase 3 (Cas3) from control (Villin-CreERt) and YAP/TAZ double conditional knockout (Villin-CreERt Yapfl/fl Tazfl/fl) animals treated with tamoxifen. Tissues were harvested and analyzed at day 7 after first tamoxifen injection. Loss of YAP/TAZ leads to a mild increase in apoptosis (Cas3+) in crypt base stem cells, but no overall effect on the morphology of the small intestine. Arrows point to stem cells (YAP nuclear staining), while arrows point to apoptotic cells (Cas3 staining). N > 10 animals per genotype. D) Murine large intestines immunostained for YAP or cleaved caspase 3 (Cas3 ) from control (Villin-CreERt) and YAP/TAZ double conditional knockout (Villin-CreERt Yapfl/fl Tazfl/fl) animals treated with tamoxifen. Tissues were harvested and analyzed at day 7 after first tamoxifen injection. Loss of YAP/TAZ leads to increased apopotosis (Cas3+) and severe morphological defects in the transverse folds of the ascending colon, but does not affect morphology of the remaining colon regions. Quantitatively, 75% of n = 4 animals showed this severe phenotype."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
TAZ: 66826
Taz: 66826
Villin: 22349
YAP: 22601
Yap: 22601"
Figure legend: "A) Murine small (top) and large (bottom) intestines isolated from control (Cre negative) Lats1flox/flox Lats2flox/flox animals and Villin-CreERt Lats1flox/flox Lats2flox/flox animals treated with tamoxifen to induce homozygous deletion of Lats1/2 (dKO). Immunostaining for YAP and Ki67 shows a gradient of YAP expression along the crypt-villus axis in controls, with nuclear YAP and Ki67 positive cells restricted to the crypt base (representative images from n=5 mice). Tamoxifen treated (3 days i.p.) Villin-CreERt Lats1flox/flox Lats2flox/flox double homozygous mouse intestines show an enlarged crypt compartment after 7 days with strongly nuclear YAP immunostaining in all epithelial cells and an expanded proliferative zone marked by Ki67 positive cells. Note the gradient of YAP expression levels is maintained along the crypt-villus axis (representative images from n=5 mice for each genotype). (Right) Immunostaining for the Paneth cell marker Lyz reveals loss of this marker from the crypt base. (Bottom right) Q-PCR analysis of Wnt pathway target genes reveals that Lats1/2 dKO causes a mild reduction in Lgr5 expression, with complete loss of Olfm4."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
Lats1: 16798
Lats2: 50523
Lgr5: 14160
Olfm4: 380924
Villin: 22349"
Figure legend: "B) Axin2 mRNA expression was measured by RNAscope (red) and found to be increased in both intensity and uniformity of staining along the crypt-villus axis in Lats1/2 dKO after 3 days post i.p. injection (dpi) with tamoxifen to induce the homozygous deletion of Lats1 and Lats2. At 7dpi, Axin2 mRNA levels remain uniform along the crypt-villus axis in Lats1/2 dKO animals, although their total level has declined compared to 3dpi."
NCBI gene IDs:
"Axin2: 12006
Lats1: 16798
Lats2: 50523"
Figure legend: "D) Q-PCR analysis of YAP-TEAD and their target genes Ctgf and Cyr61 reveals that Lats1/2 dKO causes a strong induction of Ctgf and Cyr61 expression, as expected, as well as a strong induction of the common Wnt and YAP target gene CyclinD1."
NCBI gene IDs:
"Cyr61: 16007
Ctgf: 14219
CyclinD1: 12443
Lats1: 16798"
Figure legend: "B) RNAscope in situ hybridisation analysis of TEAD1/2/4 mRNA expression from control (Cre negative) Lats1flox/flox Lats2flox/flox animals and Villin-CreERt Lats1flox/flox Lats2flox/flox animals treated with tamoxifen to induce homozygous deletion of Lats1/2 (dKO). Tamoxifen treated (3 days i.p.) Villin-CreERt Lats1flox/flox Lats2flox/flox double homozygous mouse intestines show elevated expression of TEAD1 and TEAD4 in both the small intestine (top) and colon (bottom). Tissues were harvested and analyzed at day 7 after first tamoxifen injection. (n=5 animals for each genotype)"
NCBI gene IDs:
"Cre: 2777477
ER: 2099
Lats1: 16798
Lats2: 50523
TEAD1: 21676
TEAD4: 21679
Villin: 22349"
Figure legend: "C) Q-PCR analysis reveals a progressive increase in the YAP-TEAD target gene Ctgf in Lats1/2 dKO intestines at 3-days and 7-days post i.p. injection with tamoxifen, as well as similarly increased TEAD1 and TEAD4 expression, confirming that TEAD1 and TEAD4 are YAP-responsive genes. Notably, Axin2 and TEAD2 exhibit a mild but progressive decline over 3 to 7 days after Lats1/2 deletion, consistent with Axin2 being a known Wnt-specific target gene, and with Wnt signalling remaining active in Lats1/2 dKO intestines."
NCBI gene IDs:
"Axin2: 12006
Ctgf: 14219
Lats1: 16798
TEAD1: 21676
TEAD2: 21677
TEAD4: 21679"
Figure legend: "A) RNAscope in situ hybridisation analysis of TEAD1/2/4 mRNA expression from control (wild-type) and ApcMin mutant tumours from the small intestine (top) and colon (bottom). All three genes were strongly induced within the ApcMin mutant tumours, with TEAD2 being most strongly induced in colon ApcMin tumours (n=3 animals with multiple tumours within the small and large intestines)."
NCBI gene IDs:
"Apc: 11789
TEAD1: 21676
TEAD2: 21677"
Figure legend: "C) Wnt activation in Apc5 homozygous mutant organoids drives a drastic shift in organoid morphology from budding structures to cystic spheres and increases the YAP protein level. Scale bar is 250µm. n &gt; 10 organoids in each experiment. Quantification of YAP protein levels from crypt and villus regions of control organoids as well as from the regions bounded by rectangles in Apc5 organoids are shown on the right, with statistical significance in an unpaired 2-tailed t-test."
NCBI gene IDs:
"Apc: 11789"
Figure legend: "A) Src family kinase inhibition via Dasatinib treatment in control organoids enforces YAP cytoplasmic localisation after 4h of treatment in organoids cultured for 3d. n &gt; 10 organoids in each experiment. B) Quantification of the percentage of nuclear YAP cells per control crypt bud as shown in panel (A), error bars show 1 SD (**** p &lt;0.0001). n &gt; 10 organoids per condition (top). Quantification of the nuclear/cytoplasmic ratio of YAP per cell in Apc5 mutant organoids as shown in panel (C). n &gt; 10 organoids per condition (bottom). Statistical analysis was performed with an unpaired student's t-test. C "
NCBI gene IDs:
"Apc: 11789"
Figure legend: "C) Src family kinase inhibition via Dasatinib or eCF506 treatment in Apc5 organoids causes relocalisation of YAP to the cytoplasm. n &gt; 10 organoids in each experiment. Q-PCR analysis of the YAP-TEAD target gene Ctgf reveals a strong inhibition of mRNA expression upon treatment with eCF506 (relative expression, error bars = 1 SD). Statistical analysis was performed with an unpaired student's t-test."
NCBI gene IDs:
"Ctgf: 14219"
Figure legend: "B) Control VillinCreERt mouse small intestine immunostained for YAP shows a gradient of expression with mostly cytoplasmic localisation. Irradiation with 14Gy of X-rays drives strong nuclear localisation of YAP along the entire crypt-villus axis. Deletion of Src within VillinCreERt Srcflox/flox intestines prevents YAP nuclear localisation and normal regeneration after 14Gy irradiation. Arrows point to villar cells with nuclear YAP localisation. Lower panels show high-mag views of a different example than those shown in upper panels."
NCBI gene IDs:
"Cre: 2777477
ER: 2099
Src: 20779
Villin: 22349"
Figure legend: "A) Control and irradiated murine small intestines analysed with RNAscope in situ hybridisation for Myc mRNA (black arrows point to mRNA granules in the control) and by immunostaining for Sox9 protein reveals an expansion in the expression domain of both genes. Nuclear localisation of beta-catenin is readily detectable after irradiation. n = 3 samples per condition, quantification shown in the insets, error bars = 1 SD. Statistical analysis was performed with an unpaired student's t-test. Similar results were obtained at 3dpi and 5dpi."
NCBI gene IDs:
"Myc: 17869"
Figure legend: "B) Q-PCR analysis of YAP, TEADs and their target genes Ctgf and Cyr61 over a 4 days post irradiation time course at 12Gy. Note the strong upregulation of TEAD4, Ctgf, and Cyr61. In contrast, Axin2, TEAD2 and Sox9 mRNA expression levels are only moderately altered, confirming that Wnt signalling remains active upon irradiation-induced YAP-TEAD activation. n = 4 biological replicates per time point. Statistical analysis was performed with unpaired t-test compared to the control 0Gy condition, * p&lt;0.05, ** p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"Axin2: 12006
Cyr61: 16007
Ctgf: 14219
Sox9: 20682
TEAD2: 21677
TEAD4: 21679
YAP: 22601"
Figure legend: "D) Q-PCR analysis of Wnt and YAP target gene expression in intestinal crypts isolated at 1 day after 12Gy irradiation. Note the inhibition of Axin2, Sox9, YAP, Ctgf, Cyr61, and TEAD family expression upon treatment with Porcupine inhibitor LGK974. The mild reduction of Sox9 mRNA expression by LGK974 at 1 day after irradiation (1dpi) becomes more pronounced at 3 days after irradiation (3dpi). n = 4 biological replicates per condition, statistical analysis was performed with Student's 2-tailed t-test, error bars = 1 SD, * p&lt;0.05, ** p&lt;0.01, ***p&lt;0.001."
NCBI gene IDs:
"Axin2: 12006
Cyr61: 16007
Ctgf: 14219
Sox9: 20682
YAP: 22601"
Figure legend: "(e; HA-GluN2Bct-CTMm; P = 0.785 compared to control (white bar); F(4,35) = 0.432, 8 independent experiments from 8 separate cell cultures and transfections). Levels of cDAPK1 or wtDAPK1 cotransfected with pcDNA3.0 vector (0:1, white bar) represent the control values, arbitrarily set as 1. *P 0.05, **P 0.01, ***P 0.001, compared with the control; n.s., not significant. Bars represent relative means ± s.e.m. Full-length blots are presented in Supplementary Figure 9. Membranes reprobed for β-actin were used as loading controls. One-way ANOVA with Fisher least significant difference test."
NCBI gene IDs:
"GluN2B: "
Figure legend: "(b) Design and production of TAT-GluN2Bct-CTM and TAT-GluN2Bct peptides (left) using an E. coli expression system. Coomassie blue staining after SDS-PAGE assessed their purity (right); left lane, size marker."
NCBI gene IDs:
"TAT: "
Figure legend: "(c) Bath application of TAT-GluN2Bct-CTM (200 μM; n = 9), but not TAT-GluN2Bct (200 μM; n = 6), knocked down activated DAPK1, and this was prevented by NH4Cl (20 mM; n = 5; one-way ANOVA; P 0.001, F(5,36) = 10.891) and dose- (d, doses in μM; n = 4; P 0.001; F(6,21) = 18.14) and time-dependent (e; P 0.001; F(8,44) = 12.074). (f) A single pretreatment of TAT-GluN2Bct-CTM (sing; 200 μM, 60 min before and during the 30-min NMDA stimulation) produced a transient reduction of DAPK1, returning to baseline within 7 h (n = 4; P = 0.888), and an additional dose of the peptide after NMDA washout resulted in a persistent decrease in DAPK1 up to 7 h (mult; n = 4; ΔΔP = 0.002). One-way ANOVA; P 0.001, F(4,15) = 10.389."
NCBI gene IDs:
"TAT: "
Figure legend: "(g) Synthetic peptides TAT-GluN2B and TAT-GluN2BCTM."
NCBI gene IDs:
"TAT: "
Figure legend: "(h) TAT-GluN2BCTM (25 μM; n = 5; P = 0.001), but not control TAT-GluN2B (25 μM; n = 4; P = 0.223), decreased native DAPK1, and this was prevented by NH4Cl (20 mM; n = 5; P = 0.302). One-way ANOVA, P 0.001, F(5,24) = 13.591. Relative levels of DAPK1 were normalized to those in the saline control group and compared to the saline control (white bar; *P 0.05, **P 0.01 and ***P 0.001) or NMDA-treated group (gray bar; ΔP 0.05, ΔΔP 0.01 and ΔΔΔP 0.001). Membranes reprobed for β-actin were used as a loading control. Bars represent relative means ± s.e.m. Sample sizes represent number of individual experiments. Full-length blots are presented in Supplementary Figure 9."
NCBI gene IDs:
"TAT: "
Figure legend: "(a) Top, the synthetic cell-penetrating α-synuclein targeting peptide TAT-βsynCTM and its control TAT-βsyn. Middle, immunoblots demonstrate that TAT-βsynCTM (25 μM; n = 5), but not the CTM-lacking control peptide TAT-βsyn (25 μM; n = 5), specifically decreased the targeted endogenous α-synuclein (one-way ANOVA, Tukey post hoc, P 0.001, F(3,16) = 12.435), without affecting the level of unrelated control protein PSD-95 at 4 h (bottom), and this reduction was prevented in the presence of NH4Cl (20 mM; n = 5). Sample sizes represent individual experiments from at least 3 separate primary cultures. ("
NCBI gene IDs:
"TAT: 
βsyn: "
Figure legend: "(b) Bath application of 100 μM TAT-GluN2Bct-CTM (36.54 ± 7.1% of control; n = 8; P = 0.001), but not TAT-GluN2Bct (89.13 ± 10.78%; n = 7; P = 0.311), 60 min before and during H2O2 treatment (300 μM; 30 min) knocked down DAPK1 at 2 h after washout, and this effect was rescued by NH4Cl (20 mM; n = 8; **P = 0.003 to control; P = 0.106 to H2O2-treated). One-way ANOVA, F(4,34) = 11.628, P 0.001. Bars represent DAPK1 levels relative to saline control group. β-actin was used as a loading control."
NCBI gene IDs:
"TAT: "
Figure legend: "(c) LDH assay revealed that H2O2 treatment (300 μM; 30 min) resulted in a significant increase in neuronal death 12 h after treatment (n = 8; 2.50 ± 0.12; P 0.001 to control), which was rescued by breaking down H2O2 with catalase (100 U in 10 μl phosphate-buffered saline; n = 4; 1.17 ± 0.02; P = 0.001 to H2O2 group). H2O2-induced neurotoxicity was significantly reduced by TAT-GluN2Bct-CTM (50 μM; applied 60 min before and maintained throughout the experiments; n = 9; 1.56 ± 0.08; P = 0.001 to H2O2 group), but not by TAT-GluN2Bct (50 μM; n = 9; 2.63 ± 0.10; P = 0.105 to H2O2 group) or the NMDAR antagonist APV (1 μM; n = 4; 2.16 ± 0.14; P = 0.169 to H2O2 group). NH4Cl abolished the neuroprotective effect of TAT-GluN2Bct-CTM (n = 4; 2.39 ± 0.27; P = 0.538 compared to H2O2 group). One-way ANOVA, P 0.001, F(6,41) = 26.842. ***,ΔΔΔP 0.001; bars represent relative mean values ± s.e.m. normalized to the saline control (white bar, arbitrarily set as 1). n represents individual experiments from at least 3 separate primary cultures. Full-length blots are presented in Supplementary Figure 9."
NCBI gene IDs:
"TAT: "
Figure legend: "(d) Immunoblots demonstrated specific DAPK1 knockdown in the infarct (Ipsi) but not contralateral (Contra) side following application of TAT-GluN2BCTM (10 mg per kilogram, i.v.; n = 3; t(4) = 14.459, P 0.001) but not TAT-GluN2B (10 mg per kilogram, i.v.; n = 3; t(4) = 0.739, P = 0.501). β-actin was used as a loading control; two-tailed Student's t-test ***P 0.001; n.s., not significant."
NCBI gene IDs:
"GluN2B: 
TAT: "
Figure legend: "(e) HE (left) and immunohistochemical DAPK1 (right) staining of adjacent brain sections. Compared with those in saline (top) and TAT-GluN2B-treated (middle) controls, TAT-GluN2BCTM treatment (bottom) selectively reduced infarct area (left) and DAPK1 levels (right) ipsilaterally. Black outlining delineates infarct areas as visualized with HE staining."
NCBI gene IDs:
"GluN2B: 
TAT: "
Figure legend: "(f) Left, brain sections stained with Fluorojade B in rats injected with saline (n = 6), TAT-GluN2B (n = 5) or TAT-GluN2BCTM (n = 5) after treatment as shown in a. Right, quantification of cellular damage by counting the number of Fluorojade B-positive cells in each 640 × 640 pixel image at 10× magnification. TAT-GluN2BCTM (10 mg per kilogram) displayed more prominent neuroprotection in the cortex (P 0.001) and striatum (P 0.001) as compared to TAT-GluN2B (10 mg per kilogram). Cortex: H(2) = 41.235; P 0.001; striatum: H(2) = 38.808; P 0.001. Kruskal-Wallis ANOVA on ranks with Dunn's post hoc; bars represent relative means ± s.e.m., ***,ΔΔΔP 0.001. n values represent tissue from 3 animals collected from at least 2 litters. Scale bars 1 mm in b,c,e, 20 μm in f. Full-length blots are presented in Supplementary Figure 9."
NCBI gene IDs:
"GluN2B: 
TAT: "
Figure legend: "Relative expression  and co-localization  of IL6  mRNA and  IL-6  Signaling Scoring in integrated skin  cells by blended UMAPs."
NCBI gene IDs:
"IL6: 16193
IL-6: 16193"
Figure legend: "Quantification by real-time qPCR analysis of Il6  mRNA in the skin  of naïve and irradiated  (15Gy) WT mice  at indicated times, (n=3-6). Data information: Data represent mean ± SD (B, *P&lt;0.05, **P&lt;0.01, **P&lt;0.01, ****P&lt;0.001 by one-way ANOVA with Dunnet's multiple comparisons test (B), five independent experiments."
NCBI gene IDs:
"Il6: 16193"
Figure legend: "Quantification of ventral hair loss area 21 days post-IR   (15Gy) in WT and IL-6-/- mice, (n=11). Photographic images showing hair  loss  (area demarked by yellow dashed line) 21 days post-IR   (15 Gy) in wild type (WT) and IL-6-/- mice. Data information: Data represent mean ± SD C, *P&lt;0.05, **P&lt;0.01, **P&lt;0.01, ****P&lt;0.001 by , two-tailed Mann-Whitney test (C, ; five independent experiments."
NCBI gene IDs:
"IL-6: 16193"
Figure legend: "Histochemical  (H&amp;E) and immunostaining  (red) showing skin morphology, and T cell  (CD3+) and neutrophil  (Ly6b+) infiltration in WT or IL-6-/- mice  before (naïve), and 14 days (H&amp;E, Ly6b+), or 21 days (CD3+) post-IR. Infiltrating CD3+ T cells  (yellow arrows) within degenerating hair follicles (HF; dashed lines) are indicated. Scale bars, 100 μm. Quantification of epidermal  thickness at 14 days post-IR  (n = 5-8). Kinetics  of neutrophil  (Ly6b+) (n = 3-9) and  T cell (CD3+) (n = 3) infiltration. Data information: Data represent mean ± SD F, mean ± SD (G). *P&lt;0.05, **P&lt;0.01, **P&lt;0.01, ****P&lt;0.001 by two-tailed Mann-Whitney test F, or two-way ANOVA with Bonferroni's posthoc multiple comparisons test (G); five independent experiments."
NCBI gene IDs:
"IL-6: 16193"
Figure legend: "Quantification by  real-time qPCR analysis of Tnfa, Ccl3, and Ifng  mRNAs in whole skin  of WT and IL-6-/- mice  at indicated times  post-IR  (15Gy), (n = 5-7). Data information: Data represent mean ± SD H) *P&lt;0.05, **P&lt;0.01, **P&lt;0.01, ****P&lt;0.001 by , two-tailed Mann-Whitney test H), five independent experiments."
NCBI gene IDs:
"Ccl3: 20302
Ifng: 15978
IL-6: 16193
Tnfa: 21926"
Figure legend: "Western blot analysis shows phosphorylated STAT3  (p-Stat3), Akt  (p-Akt)), Erk1  and Erk2  (p-Erk1/2)), and β-Actin in the skin  of WT, IL-6-/-, and IL-1R-/- mice at indicated times  (days) post-IR  (15Gy)."
NCBI gene IDs:
"IL-1R: 16177
IL-6: 16193"
Figure legend: "UMAP plots depicting relative expression   and localization  of Il1a  and Il1b  mRNAs in irradiated  and naïve skin-derived cell clusters."
NCBI gene IDs:
"Il1a: 16175
Il1b: 16176"
Figure legend: "Photographic images showing hair  loss 14 days post-IR   (15 Gy) in WT and IL-1R-/- mice. Quantification of ventral hair loss area 14 days post-IR   (15Gy) in WT and IL-1R-/- mice, (n = 4). Data information: Data represent mean ± SD. *P&lt;0.05, by two-tailed Mann-Whitney test (C) five independent experiments."
NCBI gene IDs:
"IL-1R: 16177"
Figure legend: "UMAP plots depicting relative expression   and localization  of Ccr6  and Ccl20  mRNAs in irradiated  and naïve skin-derived cell clusters. Quantification by real-time qPCR analysis of Ccr6  and Ccl20  mRNAs in whole skin  of naïve and irradiated  (15Gy) WT and IL-6-/- mice  at indicated times  post-IR, (n=5-7). Data information: Data represent mean ± SD. *P &lt; 0.05, **P &lt; 0.01 by Mann-Whitney test Two independent experiments."
NCBI gene IDs:
"Ccl20: 20297
Ccr6: 12458
IL-6: 16193"
Figure legend: "Photographic images showing the development of radiodermatitis  and alopecia in irradiated  (15 Gy) WT and CCR6-/- mice at indicated times  post-IR. Escalating radiodermatitis, which manifests on day 7-10 as serous exudates  on the chin  and neck, is visible in both strains and progresses in WT mice  to alopecia  by day 14 post-IR  but resolves spontaneously in the CCR6-/- mice. Quantification (lower right) of the area of hair  loss at 14 days post-IR, (n = 6). Data information: Data represent mean ± SD. *P &lt; 0.05, **P &lt; 0.01 by Mann-Whitney test Two independent experiments."
NCBI gene IDs:
"CCR6: 12458"
Figure legend: "Histochemical staining  (H&amp;E) and immunostaining  (red) show morphology  and infiltration of neutrophils  (Ly6b+) and T cells (CD3+) (red arrows) to the skin  and to the hair follicle (HF) (dashed lines) in WT or CCR6-/- mice  14 days post-IR. Scale bars, 50 μm (black) and 25 μm (red). Quantification of epidermal  thickness, neutrophils  (Ly6b+), total T cell  (CD3+) infiltration to the skin, and (F) CD3+ cells infiltration to the  hair follicle in (D), (n = 5-6). Data information: Data represent mean ± SD. *P &lt; 0.05, **P &lt; 0.01 by two-tailed Student's t test (E-Ly6b, by Mann-Whitney test Two independent experiments."
NCBI gene IDs:
"CCR6: 12458"
Figure legend: "Quantification by real-time qPCR analysis of Il6, Il17a, Ccl20, and Ccl3  mRNAs in the skin  of naïve WT and irradiated  (15Gy) WT and CCR6-/- mice  at 14 days post-IR, (n = 3-5). Data information: Data represent mean ± SD. *P &lt; 0.05, **P &lt; 0.01 by Mann-Whitney test Two independent experiments."
NCBI gene IDs:
"Ccl20: 20297
Ccl3: 20302
CCR6: 12458
Il17a: 16171
Il6: 16193"