system: [SYSTEM]

user: 3. 结果 3.1. 兔子口服蝉花发酵菌丝体安全性评估 血液学分析结果如表1。在所有血液学分析项目中，蝉花发酵菌丝体低剂量组与高剂量组，与空白组以及对照组间相比，均无统计差异(p > 0.05)。血清生化分析结果如表2。高剂量组的肌酸酐(creatinine)与对照组相比较低(p < 0.05)，其余血清生化生析项目于低剂量组及高剂量组中，均与空白组及对照组间无统计差异。组织病理学检查结果如表3。空白组、对照组、低剂量组及高剂量组，肝脏及肾脏均无明显与试验物质有关之组织病理变化。 Table 1 Hematology of rabbits after administration of Cordyceps cicadae myceli Item 空白组 对照组 低剂量组 高剂量组 Naïve Vehicle Low-dose 2.5 mg/kg b.w High-dose 25 mg/kg b.w. WBC (10 3 /μL) 12.03 ± 1.24 12.69 ± 1.89 12.20 ± 3.24 12.02 ± 2.23 RBC (10 6 /μL) 9.48 ± 0.47 9.16 ± 0.25 9.63 ± 0.34 9.66 ± 0.65 Hemoglobin (g/dL) 16.52 ± 0.50 16.02 ± 0.43 16.44 ± 0.36 16.38 ± 0.79 Hematocrit (%) 49.42 ± 1.84 48.08 ± 1.69 49.34 ± 0.62 48.68 ± 1.89 MCV (fL) 52.24 ± 2.68 52.56 ± 2.90 51.27 ± 1.32 50.52 ± 2.39 MCH (pg) 17.44 ± 0.63 17.50 ± 0.59 17.10 ± 0.69 17.00 ± 0.51 MCHC (g/dL) 33.44 ± 0.70 33.34 ± 0.89 33.32 ± 0.76 33.62 ± 0.74 Platelet (10 3 /μL) 960.0 ± 104.7 969.2 ± 97.1 955.8 ± 150.7 992.8 ± 167.8 Neutrophil (%) 20.66 ± 6.60 22.60 ± 8.54 20.50 ± 9.04 23.50 ± 5.73 Lymphocyte (%) 73.16 ± 6.35 69.84 ± 9.58 66.86 ± 21.69 69.80 ± 6.61 Monocyte (%) 4.80 ± 0.46 5.88 ± 1.27 5.08 ± 1.27 5.22 ± 0.92 Eosinophil (%) 1.26 ± 0.55 1.54 ± 0.49 1.40 ± 0.46 1.36 ± 0.78 Basophil (%) 0.12 ± 0.04 0.14 ± 0.05 0.16 ± 0.09 0.12 ± 0.04 表1. 血液学分析 所有数据均以平均值 ± 标准偏差表示，n = 5。Data expressed as mean ± standard deviation (n = 5). WBC， white blood cell. RBC， red blood cell. MCV， mean corpuscular volume. MCH， mean corpuscular hemoglobin. MCHC， mean corpuscular hemoglobin concentration. Table 2 Serum biochemistry of rabbits after administration of Cordyceps cicadae myceli Item 空白组 对照组 低剂量组 高剂量组 Naïve Vehicle Low-dose 2.5 mg/kg b.w High-dose 25 mg/kg b.w. Glucose (mg/dL) 396.2 ± 101.4 407.4 ± 90.1 392.4 ± 43.7 342.0 ± 74.9 BUN (mg/dL) 19.1 ± 2.2 19.2 ± 3.3 18.8 ± 1.4 19.4 ± 2.6 Creatinine (mg/dL) 1.18 ± 0.11 1.33 ± 0.19 1.18 ± 0.08 1.09 ± 0.11* AST (U/L) 62.4 ± 98.2 23.4 ± 6.1 19.6 ± 4.3 19.4 ± 3.5 ALT (U/L) 68.6 ± 36.3 57.2 ± 15.6 60.2 ± 13.6 48.6 ± 11.2 Total protein (g/dL) 6.1 ± 0.2 6.2 ± 0.3 5.9 ± 0.3 5.9 ± 0.4 Albumin (g/dL) 4.06 ± 0.17 4.02 ± 0.15 3.94 ± 0.23 4.04 ± 0.26 ALP (U/L) 125.8 ± 12.1 116.6 ± 23.7 181.4 ± 82.8 147.6 ± 27.6 Cholesterol (mg/dL) 71.0 ± 15.9 85.0 ± 23.4 65.0 ± 21.8 68.8 ± 19.8 Triglyceride (mg/dL) 63.2 ± 8.2 117.8 ± 96.2 59.2 ± 17.4 68.6 ± 13.0 Calcium (mg/dL) 14.6 ± 0.3 14.7 ± 0.6 14.6 ± 0.4 14.7 ± 1.1 Phosphorus (mg/dL) 8.3 ± 0.9 8.5 ± 0.5 8.2 ± 0.3 8.4 ± 0.9 Sodium (meq/L) 142.8 ± 2.3 142.4 ± 2.7 143.2 ± 0.8 143.0 ± 4.4 表2. 血清生化分析 所有数据均以平均值 ± 标准偏差表示，n = 5。*与对照组比较具显著性差异(p < 0.05)。Data expressed as mean ± standard deviation (n = 5). BUN， blood urea nitrogen. AST， aspartate transaminase. ALT， alanine transaminase. ALP， alkaline phosphatase. *Significantly different from the control group (P < 0.05). Table 3 Histopathological changes of rabbits after administration of Cordyceps cicadae myceli Organ Lesions 空白组 对照组 低剂量组 高剂量组 Naïve Vehicle Low-dose 2.5 mg/kg b.w High-dose 25 mg/kg b.w. Liver Mixed type inflammatory cell infiltration， minimal， random 1/5 5/5 2/5 2/5 Kidney Mononuclear cell infiltration， minimal， interstitial - 2/5 - 1/5 表3. 肝脏及肾脏病理检查 3.2. 口服蝉花发酵菌丝体降眼压评估 兔子喂食样品D3及D7后第1及第3小时皆可观察到眼压下降(如图1)，喂食蝉花发酵菌丝体D3后第0、1、3小时，兔子眼压分别为12.60 ± 0.28、12.07 ± 0.34、11.98 ± 0.27，在第1及第3小时的眼压变化量为−0.53 ± 0.15、−0.62 ± 0.20，其下降百分比分别为−4.25% ± 1.28%、−4.89% ± 1.59% (表4)。喂食蝉花发酵菌丝体D7后第0、1、3小时，兔子眼压分别为12.22 ± 0.09、11.44 ± 0.33、11.73 ± 0.34，在第1及第3小时的眼压变化量为−0.78 ± 0.24、−0.49 ± 0.33，其下降百分比分别为−6.42% ± 1.98%、−3.99% ± 2.72% (表4)。