system: [SYSTEM]

user: 3. 结果 3.1. 肿瘤患者外周血T细胞亚群与正常对照组比较 与正常对照组对比，肿瘤患者外周血淋巴细胞总数、CD3总T细胞计数、CD8抑制/杀伤T细胞计数及CD4 + /CD8 + 比值均有所下降，且差异具有统计学意义(P < 0.05) (见表1)。 3.2. 放化疗前后T淋巴细胞亚群水平的差异性比较 放化疗后T淋巴细胞亚群不论是在T淋巴细胞亚群正常组及低下组其水平均较放化疗前有所下降，且差异具有统计学意义(P < 0.05) (见表2)。 3.3. 放疗前T淋巴细胞亚群水平与放射性损伤分级在化疗组与未化疗组之间的差异性比较 放疗前T淋巴细胞亚群水平在合并化疗组发生I/II级、III/IV级放射性损伤的风险均较未合并化疗组有所增加，差异具有统计学意义(P < 0.05) (见表3)。 3.4. 放化疗前T淋巴细胞亚群水平与不同预后组之间的关系 放化疗前T淋巴细胞亚群水平CD3、CD4、CD8及CD4 + /CD8 + 按照正常及低下进行分组(以我院流式细胞仪参数作为参考标准)，分析其各组与不同预后组(CR， PR， SD， PD)之间的关系，因不同预后组为等 Table 1 The difference of peripheral blood T cell subsets between tumor group and control grou Groups n Total lymphocyte count CD3 + count CD4 + count CD8 + count CD4/CD8 Tumor group 182 1522.32 ± 611.4 1043.73 ± 490.05 554.97 ± 311.83 473.94 ± 275.92 1.31 ± 0.67 control group 144 1563.95 ± 450.99 1092.46 ± 400.99 828.00 ± 307.16 610.15 ± 208.38 1.45 ± 0.55 表1. 肿瘤患者外周血T 细胞亚群与正常对照组比较 Table 2 The level of T lymphocyte subgroup differences before and after the radiation therap T lymphocyte subgroup level Before n 1 After n 2 Chi-square value P value Total lymphocyte count Normal group 92 70 484.797 0.000 Lower group 90 112 CD3 + count Normal group 101 74 484.808 0.000 Lower group 81 108 CD4 + auxiliary/induction T cell counts Normal group 120 91 489.136 0.000 Lower group 62 91 CD8 + inhibition/killer T cell count Normal group 133 98 485.005 0.000 Lower group 49 84 CD4 + /CD8 + Normal group 118 104 504.611 0.000 Lower group 64 78 表2. 放化疗前后T淋巴细胞亚群水平的差异性比较 Table 3 Radioactive damage grade differences in T lymphocyte subgroup level between chemotherapy group and no chemotherapy group before radiation therap T lymphocyte subgroup level before radiation therapy Radioactive damage grade Grade I/II Grade III/IV Chi-square value P value Total lymphocyte count Chemotherapy group n 1 = 77 28 16 149.000 0.000 No chemotherapy group n 2 = 105 61 44 CD3 + count Chemotherapy group n 1 = 80 43 37 182.000 0.000 No chemotherapy group n 2 = 102 44 58 CD4 + auxiliary/induction T cell counts Chemotherapy group n 1 = 86 40 46 101.758 0.000 No chemotherapy group n 2 = 96 56 40 CD8 + inhibition/killer T cell count Chemotherapy group n 1 = 83 38 45 182.000 0.000 No chemotherapy group n 2 = 99 66 33 CD4 + /CD8 + Chemotherapy group n 1 = 68 36 32 223.965 0.000 No chemotherapy group n 2 = 114 74 40 表3. 放疗前T淋巴细胞亚群水平与放射性损伤分级在化疗组与未化疗组之间的差异性比较 级资料，故统计学方法采用两独立样本的非参数检验Mann-Whitney检验，P < 0.05被视为差异具有统计学意义(见表4)。 表4的统计分析结果初步显示放化疗前T淋巴细胞亚群中淋巴细胞总数、CD3总T细胞计数、CD4辅助/诱导T细胞计数与不同预后组(CR， PR， SD， PD)之间存在统计学差异，故进一步进行组间两两比较(表5、表6、表7)，组间两两比较采用卡方检验，不满足条件时采用Fisher精确概率法(P < 0.01被认为 Table 4 The relationship between different prognosis groups with the T lymphocyte subgroup level before chemoradiatio T lymphocyte subgroup level before chemoradiation therapy Different prognosis groups CR PR SD PD Z-value P value Total lymphocyte count Normal group n = 100 41 27 20 12 −3.409 0.001 Lower group n = 82 22 20 30 10 CD3 + count Normal group n = 93 32 28 22 11 −2.991 0.003 Lower group n = 89 29 15 35 10 CD4 + auxiliary/induction T cell counts Normal group n = 109 49 26 24 10 −5.642 0.000 Lower group n = 73 23 8 28 14 CD8 + inhibition/killer T cell count Normal group n = 109 49 26 24 10 −2.176 0.030 Lower group n = 73 23 8 28 14 CD4/CD8 Normal group n = 104 44 25 23 12 −0.866 0.486 Lower group n = 78 33 15 20 10 表4. 放化疗前T淋巴细胞亚群水平与不同预后组之间的关系 Table 5 The total number of lymphocyte level differences between different prognosis ways before chemoradiatio Prognosis way The lymphatic total Normal group Lower group Chi-square value P value CR/PR CR 21 12 35.092 0.000 PR 20 10 CR/SD CR 21 12 69.624 0.000 SD 6 20 CR/PD CR 21 12 54.978 0.000 PD 5 10 PR/SD PR 10 10 26.328 0.000 SD 6 20 PR/PD PR 10 10 9.613 0.009 PD 5 10 SD/PD SD 6 20 38.132 0.000 PD 5 10 表5. 放化疗前淋巴总数水平与不同预后组组间差异性比较 Table 6 CD3+ total T cell count differences of between different prognosis ways before chemoradiatio Prognosis way CD3 + total T cell count Normal group Lower group Chi-square value P value CR/PR CR 16 8 57.247 0.000 PR 20 10 CR/SD CR 16 8 58.927 0.000 SD 9 18 CR/PD CR 16 8 38.915 0.000 PD 7 6 PR/SD PR 20 10 66.801 0.000 SD 9 18 PR/PD PR 20 10 46.181 0.000 PD 7 6 SD/PD SD 9 18 43.359 0.000 PD 7 6 表6. 放化疗前CD3总T细胞计数与不同预后组组间差异性比较 Table 7 CD4+ auxiliary/induction differences of between different prognosis ways before chemoradiatio Prognosis way CD4 + auxiliary/induction Normal group Lower group Chi-square value P value CR/PR CR 19 13 60.329 0.000 PR 14 8 CR/SD CR 19 13 61.646 0.000 SD 12 10 CR/PD CR 19 13 57.669 0.000 PD 4 14 PR/SD PR 14 8 48.345 0.000 SD 12 10 PR/PD PR 14 8 11.901 0.001 PD 4 14 SD/PD SD 12 10 43.372 0.000 PD 4 14 表7. 放化疗前CD4辅助/诱导T细胞计数与不同预后组组间差异性比较 差异具有统计学意义)。