system: [SYSTEM]

user: 3. 结果与分析 3.1. 硒和杀菌剂及复配组合对水稻纹枯病菌毒力作用 结果表明(表1)，亚硒酸钠对水稻纹枯病菌的抑制效果较差，EC 50 值为9.8693 μg/mL。硒与咪鲜胺、戊唑醇和吡唑醚菌酯三种杀菌剂复配的各个组合增效系数均小于0.5，表现出拮抗作用。硒与苯醚甲环唑各复配组合A、B、C、D和E对水稻纹枯病菌均表现出较强的增效作用，增效系数分别为19.9939、25.1179、25.5484、7.5766和29.2560，均明显大于1.5。 Table 1 Inhibitory effect of the combination of selenium and fungicides on Rhizoctonia solan 药剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硒 y = 3.9503 + 1.0557x 0.9786 9.8693 (5.0085~15.8976) 吡唑醚菌酯 y = 5.4601 + 0.6686x 0.9774 0.2050 (0.0803~0.5235) 苯醚甲环唑 y = 4.4224 + 0.4327x 0.9826 21.6114 (9.5481~48.9169) 戊唑醇 y = 5.6024 + 0.6614x 0.9565 0.1228 (0.0277~0.5451) 咪鲜胺 y = 4.6753 + 0.7005x 0.9706 2.9076 (1.5729~5.3751) A y = 4.8828 + 0.3502x 0.9749 1.0809 (0.4849~2.4093) 19.9939 B y = 4.9050 + 0.4031x 0.9945 0.8604 (0.8604~0.8604) 25.1179 硒：苯醚甲环唑 C y = 4.5478 + 0.6056x 0.9705 0.8459 (0.1916~3.7475) 25.5484 D y = 4.6063 + 0.5206x 0.9810 2.8524 (1.6645~4.8879) 7.5766 E y = 4.9333 + 0.3936x 0.9855 0.7387 (0.3801~1.4358) 29.2560 A y = 4.0017 + 0.6052x 0.9762 27.6781 (13.6908~55.9557) 0.1051 B y = 3.1157 + 0.9652x 0.9652 58.5641 (27.6981~123.826) 0.0496 硒：咪鲜胺 C y = 2.9756 + 1.0230x 0.9848 47.6321 (29.9941~75.6422) 0.0610 D y = 3.1820 + 0.9990x 0.9441 32.3784 (11.8241~88.6634) 0.0898 E y = 3.6189 + 0.7212x 0.9544 41.1119 (18.6449~90.6513) 0.0707 A y = 4.9293 + 1.0348x 0.9820 0.5852 (0.2668~1.2836) 0.2098 B y = 5.1266 + 0.9965x 0.9547 0.3732 (0.0913~1.5257) 0.3290 硒：戊唑醇 C y = 4.8952 + 1.0869x 0.9804 0.6243 (0.2783~1.4001) 0.1967 D y = 5.1637 + 0.8859x 0.9899 0.3267 (0.1675~0.637) 0.3759 E y = 4.7922 + 1.2011x 0.9844 0.7447 (0.374~1.4827) 0.1649 A y = 4.4756 + 1.0250x 0.9933 1.6239 (1.1251~2.3439) 0.1262 B y = 4.6685 + 0.9275x 0.9738 1.1382 (0.5074~2.5532) 0.1801 硒：吡唑醚菌酯 C y = 4.5574 + 0.9235x 0.9983 1.5073 (1.2500~1.8176) 0.1360 D y = 4.6215 + 1.0278x 0.9868 1.1673 (0.6641~2.0520) 0.1756 E y = 4.3993 + 0.9581x 0.9948 2.1183 (1.5659~2.8655) 0.0968 表1. 硒和杀菌剂及复配组合对水稻纹枯病菌毒力作用 3.2. 硒和杀菌剂及复配组合对稻瘟病菌毒力作用 结果表明(表2)，亚硒酸钠对水稻稻瘟病菌的抑制效果较好，EC 50 值为0.9899 μg/mL。硒与苯醚甲环唑各复配组合以及硒与三环唑各复配组合对水稻稻瘟病菌均表现出较强的增效作用，硒与苯醚甲环唑复配组合增效系数分别为2.9510、2.1351、4.1045、1.8485和5.7502，硒与三环唑复配组合增效系数分别为2.7791、5.7252、3.0269、6.6283和1.5433，均大于1.5。 Table 2 Inhibitory effect of the combination of selenium and fungicides on Magnaporthe oryza 药剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硒 y = 6.8594 + 1.1041x 0.9866 0.9899 (0.1243~1.3213) 吡唑醚菌酯 y = 6.0466 + 0.8958x 0.9911 0.0682 (0.0323~0.1441) 苯醚甲环唑 y = 4.1158 + 1.9671x 0.9698 2.8153 (2.0377~3.8895) 戊唑醇 y = 5.7746 + 0.5484x 0.9879 0.0387 (0.0170~0.0882) 咪鲜胺 y = 5.6205 + 0.5838x 0.9941 0.0865 (0.0520~0.1437) 三环唑 y = 5.7496 + 0.7153x 0.9731 0.7437 (0.0315~0.2543) A y = 5.0770 + 1.1676x 0.9971 0.8592 (0.6402~1.1530) 0.0794 B y = 5.3103 + 1.0432x 0.9594 0.5041 (0.1355~1.8758) 0.1353 硒：吡唑醚菌酯 C y = 5.4384 + 1.2392x 0.9634 0.4428 (0.1223~1.603) 0.1540 D y = 4.7776 + 1.5296x 0.9964 1.3977 (1.0479~1.8643) 0.0488 E y = 5.1086 + 2.2754x 0.9179 0.8959 (0.1710~4.6936) 0.0761 A y = 5.0212 + 1.0390x 0.9663 0.9540 (0.4241~2.1461) 2.9510 B y = 4.8614 + 1.1540x 0.9659 1.3186 (0.6300~2.7598) 2.1351 硒：苯醚甲环唑 C y = 5.1887 + 1.1524x 0.9520 0.6859 (0.2336~2.0143) 4.1045 D y = 4.7904 + 1.1470x 0.9685 1.5230 (1.6645~4.8879) 1.8485 E y = 5.2960 + 0.9544x 0.9632 0.4896 (0.1657~1.4468) 5.7502 A y = 5.6533 + 1.0548x 0.9156 0.2403 (0.02991.9336) 0.1610 B y = 5.7330 + 1.0387x 0.9765 0.1969 (0.0654~0.5931) 0.1965 硒：戊唑醇 C y = 5.5886 + 1.1727x 0.9557 0.3149 (0.0809~1.2269) 0.1229 D y = 5.8191 + 0.8190x 0.9177 0.1000 (0.0079~1.2682) 0.3870 E y = 5.4682 + 1.0856x 0.9565 0.3705 (0.1024~1.3400) 0.1045 A y = 5.7173 + 1.0202x 0.9771 0.1981 (0.088~0.4459) 0.4366 B y = 5.5509 + 1.1260x 0.9574 0.3241 (0.1178~0.8920) 0.2669 硒：咪鲜胺 C y = 5.5741 + 1.1851x 0.9716 0.3278 (0.1473~0.7295) 0.2639 D y = 5.9445 + 1.1431x 0.9544 0.1492 (0.0408~0.5452) 0.5798 E y = 5.7423 + 0.6795x 0.9893 0.0808 (0.0396~0.1649) 1.0705 A y = 5.5649 + 0.9868x 0.9632 0.2676 (0.0739~0.9690) 2.7791 B y = 5.7134 + 0.8047x 0.9544 0.1299 (0.0230~0.7318) 5.7252 硒：三环唑 C y = 5.5100 + 0.8366x 0.9521 0.2457 (0.0540~1.1184) 3.0269 D y = 5.8678 + 0.9135x 0.8956 0.1122 (0.0066~1.9221) 6.6283 E y = 5.2858 + 0.9016x 0.9694 0.4819 (0.1323~1.683) 1.5433 表2. 硒与杀菌剂复配对水稻稻瘟病菌的抑制作用 3.3. 硒和杀菌剂及复配组合对稻曲病菌毒力作用 结果表明(表3)，亚硒酸钠对水稻稻曲病菌的抑制效果较差，EC 50 值为28.9895 μg/mL。硒与戊唑醇各复配组合对水稻稻曲病菌均表现出较强的增效作用，硒与戊唑醇复配组A、B、C、D和E增效系数分别为6.1625、7.5109、3.5137、7.3794和8.4665，均明显大于1.5。硒与其他杀菌剂复配组合对水稻稻曲病菌没有增效作用。 Table 3 Inhibitory effect of the combination of selenium and fungicides on Ustilaginoidea viren 药剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硒 y = 2.2018 + 1.9136x 0.9603 28.9895 (17.8501~47.0803) 吡唑醚菌酯 y = 5.2909 + 1.5805x 0.9756 0.6545 (0.3408~1.2573) 戊唑醇 y = 5.1651 + 1.3757x 0.9438 0.7586 (0.2635~2.1838) 咪鲜胺 y = 3.5291 + 1.3554x 0.9840 12.1686 (8.6912~17.0373) A y = 4.8724 + 0.5054x 0.9596 1.7883 (0.8369~3.8214) 0.3660 B y = 5.1287 + 0.5219x 0.9427 0.5667 (0.2070~1.5513) 1.1549 硒：吡唑醚菌酯 C y = 4.5094 + 0.8447x 0.9784 3.8094 (2.1655~6.7014) 0.1718 D y = 5.0349 + 0.5067x 0.9730 0.8533 (0.4417~1.6483) 0.7670 E y = 5.0929 + 0.4625x 0.9717 0.6296 (0.3098~1.2794) 1.0395 A y = 5.7183 + 0.7897x 0.9353 0.1231 (0.0245~0.6178) 6.1625 B y = 5.7737 + 0.7772x 0.9657 0.1010 (0.0319~0.3201) 7.5109 硒：戊唑醇 C y = 5.6150 + 0.9239x 0.9377 0.2159 (0.0540~0.8641) 3.5137 D y = 5.7358 + 0.7446x 0.9834 0.1028 (0.0467~0.2263) 7.3794 E y = 5.7263 + 0.6933x 0.9706 0.0896 (0.0287~0.2796) 8.4665 A y = 4.2089 + 0.7845x 0.9515 10.1940 (5.6539~18.3797) 1.1937 B y = 4.0358 + 0.8255x 0.9086 14.7212 (6.0875~35.6023) 0.8266 硒：咪鲜胺 C y = 4.4394 + 0.5239x 0.9829 11.7538 (5.4137~25.5188) 1.0353 D y = 4.0890 + 0.7926x 0.9327 14.1064 (0.2070~1.5513) 0.8626 E y = 4.1655 + 0.5708x 0.9483 28.9783 (6.7529~29.4675) 0.4199 表3. 硒与杀菌剂复配对水稻稻曲病菌的抑制作用 3.4. 硅和杀菌剂及复配组合对水稻纹枯病菌毒力作用 结果表明(表4)，二氧化硅对水稻纹枯病菌的抑制效果较差，EC 50 值为 95.4703 μg/ mL。硅与咪鲜胺各复配组合对水稻纹枯病菌均表现出较强的增效作用，硅与咪鲜胺复配组F、G、H和I，增效系数分别为16.7488、24.5574、34.9892和25.5726，均明显大于1.5。硅与吡唑醚菌酯复配组合F、H和I对水稻纹枯病菌表现出增效作用，增效系数分别为1.5287、2.3590和2.5754。 Table 4 Inhibitory effect of the combination of silicon and fungicides on Rhizoctonia solan 药剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硅 y = 0.0730 + 2.4888x 0.9449 95.4703 (44.0702~206.6479) 吡唑醚菌酯 y = 5.4601 + 0.6686x 0.9774 0.2050 (0.0803~0.5235) 噻呋酰胺 y = 6.1582 + 0.9304x 0.9661 0.0569 (0.0146~0.2215) 戊唑醇 y = 5.6024 + 0.6614x 0.9565 0.1228 (0.0277~0.5451) 咪鲜胺 y = 4.6753 + 0.7005x 0.9706 2.9076 (1.5729~5.3751) F y = 5.6114 + 0.7007x 0.9788 0.1341 (0.0575~0.3126) 1.5287 G y = 5.5185 + 0.7908x 0.9956 0.2211 (0.1573~0.3106) 0.9276 硅：吡唑醚菌酯 H y = 5.8322 + 0.7845x 0.9921 0.0869 (0.1916~3.7475) 2.3598 I y = 5.9715 + 0.8841x 0.9874 0.0796 (0.0387~0.1639) 2.5754 F y = 5.9255 + 0.8482x 0.9539 0.0811 (0.0185~0.355) 1.5142 G y = 5.9454 + 0.9303x 0.9638 0.0963 (0.0276~0.3359) 1.2752 硅：戊唑醇 H y = 6.1873 + 1.0918x 0.9580 0.0818 (0.0175~0.3814) 1.5012 I y = 5.8194 + 0.8631x 0.9673 0.1124 (0.0359~0.352) 1.0925 F y = 5.5888 + 0.7743x 0.9843 0.1736 (0.0875~0.3446) 0.3278 G y = 5.7991 + 0.7963x 0.9842 0.0992 (0.0457~0.2153) 0.5736 硅：噻呋酰胺 H y = 5.8635 + 0.7482x 0.9913 0.0701 (0.0379~0.1297) 0.8117 I y = 5.9152 + 0.7525x 0.9934 0.0608 (0.035~0.10552) 0.9359 F y = 5.7018 + 0.6674x 0.9977 0.1736 (0.0659~0.1196) 16.7488 G y = 5.6265 + 0.6762x 0.9991 0.1184 (0.0998~0.1406) 24.5574 硅：咪鲜胺 H y = 5.7005 + 0.6484x 0.9993 0.0831 (0.0707~0.0977) 34.9892 I y = 5.6720 + 0.7116x 0.9977 0.1137 (0.0855~0.1511) 25.5726 表4. 硅与杀菌剂复配对水稻纹枯病菌的抑制作用 3.5. 硅和杀菌剂及复配组合对稻瘟病菌毒力作用 结果表明(表5)，二氧化硅对水稻稻瘟病菌的抑制效果较差，EC 50 值为106.0198 μg/mL。硅与吡唑醚菌酯、咪鲜胺、苯醚甲环唑和三环唑四种杀菌剂各复配组合对水稻稻瘟病菌均表现出较强的增效作用，硅与吡唑醚菌酯各复配组增效系数分别为2.0419、1.5189、2.7280和2.0856，硅与咪鲜胺各复配组增效系数分别为4.3909、6.0069、3.6042和4.5526，硅与苯醚甲环唑各复配组增效系数分别为36.5623、18.1632、13.4639和5.8652，硅与三环唑各复配组增效系数分别为16.0547、15.9847、19.9375和26.4874，增效系数均大于1.5。硅与戊唑醇各复配组合对水稻稻瘟病菌表现出拮抗作用。 Table 5 Inhibitory effect of the combination of silicon and fungicides on Magnaporthe oryza 药剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硅 y = 2.0226 + 1.4700x 0.9811 106.0198 (66.8232~168.2082) 吡唑醚菌酯 y = 6.0466 + 0.8958x 0.9911 0.0682 (0.0323~0.1441) 苯醚甲环唑 y = 4.1158 + 1.9671x 0.9698 2.8153 (2.0377~3.8895) 戊唑醇 y = 5.7746 + 0.5484x 0.9879 0.0387 (0.0170~0.0882) 咪鲜胺 y = 5.6205 + 0.5838x 0.9941 0.0865 (0.0520~0.1437) 三环唑 y = 5.7496 + 0.7153x 0.9731 0.7437 (0.0315~0.2543) F y = 6.5338 + 1.0388x 0.9982 0.0334 (0.0236~0.0472) 2.0419 G y = 6.2627 + 0.9370x 0.9931 0.0449 (0.0239~0.0845) 1.5189 硅：吡唑醚菌酯 H y = 6.5504 + 0.9681x 0.9953 0.025 (0.0139~0.0450) 2.728 I y = 6.4592 + 0.9824x 0.9932 0.0327 (0.0167~0.0639) 2.0856 F y = 5.5351 + 0.9111x 0.8975 0.2586 (0.0441~1.5154) 0.1497 G y = 5.5915 + 1.3141x 0.9101 0.9101 (0.0441~1.5154) 0.0425 硅：戊唑醇 H y = 5.5329 + 0.9801x 0.9183 0.2859 (0.0765~1.6454) 0.1354 I y = 5.5567 + 0.8719x 0.8689 0.2299 (0.0281~1.8831) 0.1683 F y = 6.1981 + 0.7029x 0.9564 0.0197 (0.0029~0.1366) 4.3909 G y = 6.0154 + 0.5513x 0.9795 0.0144 (0.0036~0.0574) 6.0069 硅：咪鲜胺 H y = 6.1845 + 0.7312x 0.9634 0.024 (0.0044~0.1308) 6.0069 I y = 6.2005 + 0.6975x 0.9793 0.019 (0.0051~0.0712) 4.5526 F y = 5.5635 + 0.5062x 0.9501 0.077 (0.0174~0.3419) 36.5623 G y = 5.6619 + 0.5255x 0.9924 0.155 (0.0301~0.1005) 18.1632 硅：苯醚甲环唑 H y = 5.4087 + 0.6013x 0.9945 0.2091 (0.1419~0.3086) 13.4639 I y = 5.2277 + 0.7143x 0.8978 0.4856 (0.0707~3.2579) 5.8652 F y = 6.1213 + 0.8365x 0.9836 0.0457 (0.0172~0.1214) 16.0547 G y = 6.1618 + 0.8681x 0.9678 0.0459 (0.0115~0.1837) 15.9847 硅：三环唑 H y = 6.2681 + 0.8838x 0.9637 0.0368 (0.0078~0.1728) 19.9375 I y = 5.8194 + 0.8398x 0.9483 0.0277 (0.0038~0.2011) 26.4874 表5. 硅与杀菌剂复配对水稻稻瘟病菌的抑制作用 3.6. 硅和杀菌剂及复配组合对稻曲病菌毒力作用 结果表明(表6)，二氧化硅对水稻稻曲病菌的抑制效果较差，EC 50 值为647.8783 μg/mL。硅与吡唑醚菌酯各复配组合对水稻稻曲病菌均表现出较强的增效作用，硅与吡唑醚菌酯复配组F、G、H和I，增效系数分别为2.6170、1.5535、1.5221和2.3021均大于1.5。硅与其他杀菌剂复配组合对水稻稻曲病菌没有增效作用。 Table 6 Inhibitory effect of the combination of silicon and fungicides on Ustilaginoidea viren 混剂 配比 毒力回归方程 相关系数(r) EC 50 (μg/mL) 95%置信区间 增效系数(SR) 硅 y = 1.9774 + 1.0751x 0.9857 647.8783 (310.3742~1352.3880) 吡唑醚菌酯 y = 5.2909 + 1.5805x 0.9756 0.6545 (0.3408~1.2573) 戊唑醇 y = 5.1651 + 1.3757x 0.9438 0.7586 (0.2635~2.1838) 咪鲜胺 y = 3.5291 + 1.3554x 0.9840 12.1686 (8.6912~17.0373) F y = 6.1698 + 1.9438x 0.9419 0.2501 (0.0712~0.8798) 2.6179 G y = 5.5264 + 1.4024x 0.9973 0.4213 (0.3311~0.5362) 1.5535 硅：吡唑醚菌酯 H y = 5.4941 + 1.3479x 0.996 0.4345 (0.3244~0.5699) 1.5221 I y = 5.6855 + 1.2551x 0.9608 0.2843 (0.1028~0.7861) 2.3021 F y = 4.8379 + 0.5732x 0.9805 1.9175 (1.1414~3.2215) 0.3956 G y = 4.6455 + 0.4676x 0.9496 5.7295 (2.2530~14.5705) 0.1324 硅：戊唑醇 H y = 4.9679 + 0.4375x 0.9772 1.1843 (0.6640~2.1122) 0.6405 I y = 4.6687 + 0.4876x 0.931 4.7792 (1.6254~14.0528) 0.1587 F y = 3.4802 + 0.9812x 0.9897 35.3936 (24.8425~50.4156) 0.3438 G y = 3.7638 + 0.7993x 0.9706 35.2064 (19.2327~64.4470) 0.3456 硅：咪鲜胺 H y = 3.8865 + 0.8013x 0.9777 24.5251 (15.3366~39.2188) 0.4962 I y = 3.9353 + 0.7224x 0.9997 29.7797 (28.2171~31.4289) 0.4086 表6. 硅与杀菌剂复配对水稻稻曲病菌的抑制作用