Dragon09/odtu_chatbot huggingface dataset:
heterologous expression of natural products from microbes living in a selfsustaining environment

the increases in antimicrobial resistance and infections caused by microbes such as mucormycosis black fungus disease on covid19 patients highlight the urgent need for new antimicrobial drugs most drugs approved for clinical use are based or inspired by natural products the recurrent rediscovery of compounds when studying isolated microbes declined the research on natural products in the last decades however isolated microbes represent a small percentage of the true microbial diversity living in the environment lichens are a microbial community formed by different partners fungi green algae cyanobacteria bacteria and their metabolites present diverse biological activities including antimicrobial furthermore many of these molecules have vibrant colours and can be used as natural colorants due their slow growth and difficulties in the laboratory cultivation little is known about natural products biosynthetic genes of the multiple partners living in symbiosis the improvement in highthroughput technology and the current affordable prices for multiomics analysis metagenomics transcriptomics and bolomics are allowing a deeper study on natural environmental samples this project aims to use the knowledge obtained in a recent multiomics study and apply synthetic biotechnology bioinformatics chemistry and microbiology techniques to study novel metabolites from lichen polyketide synthase biosynthetic gene clusters have been selected and they will be cloned transformed and heterologously expressed using aspergillus oryzae the optimisation of the polyketides biosynthesis by the heterologous host will be performed and different fermentation conditions will be tested novel molecules will be chemically and biologically characterized during fungalhetext i will study novel molecules produced under symbiosis using a multidisciplinary approach with the focus to discovery new molecules and improve my science communication and leadership skills
robust and sustainable additive manufacturing of amorphous metallic alloys

bulk metallic glasses bmgs are promising materials to support the goal to make eu climateneutral by 2050 and promote the growth of many industrial sectors such as biomedical thanks to their amorphous nature bmgs offer an outstanding combination of high strength corrosionwear resistance and biocompability recently additive manufacturing am has been proposed as a solution to tackle the size limitation of the conventional casting production of bmgs still many challenges remain to develop defectfree parts with mechanical performances comparable to their ascast counterparts in addition to date no postprocesses can be applied to close porosity and improve quality without altering the amorphous state of the material the project rosama2 hosted by simap in grenoble aims to develop a robust and sustainable additive manufacturing of amorphous metallic alloys by laser powder bed fusion in situ xray microtomography will enable the 3d reconstruction of the deposited material layerbylayer using a unique miniature system designed for usage at synchrotrons the high brilliance of the radiation will reveal the quality of the powder bed the morphology of the deposited material the presence of defects in its volume and their evolution upon processing doing so strategies to eliminate porosity in situ will be proposed the printed bmgs will be evaluated to determine the role of defects structural and chemical heterogeneities on the mechanical performances by means of xray nanotomography advanced thermal analyses high resolution microscopy nanoindentation and compression testing in a final effort strategies to enhance powder recyclability and limit pickup of oxygen harmful to the glass forming ability will be developed and the role of process atmosphere composition will be considered besides strengthening eus position on the am market rosama2 will develop and consolidate dr pauzons profile and secure future position in academia and industry
unspecific peroxygenases for the oxidation of arenes

upoxarene is an interdisciplinary project combining organic synthesis biocatalysis and protein engineering to realise the elusive epoxidation of arenes in a single step using engineered unspecific peroxygenases upos the compatibility of upos with organic media will be exploited to expand their chemistry beyond the aromatic hydroxylation accessible with cytochrome p450s the utility of the method will be demonstrated through the preparation of industrially relevant products in continuous chemoenzymatic cascades in alignment with the principles set out in the msca green charter upoxarene will promote biotechnology through the citizenscience initiative peroxynauts encouraging students from all backgrounds to pursue careers in the life sciences
phase contrast stem for cryoem

cryoelectron microscopy has revolutionized the field of structural biology primarily for macromolecular structure but also for cells and tissue sectionsachieving resolutions at the limit of physical optics while widefield transmission em tem with phase contrast by defocus is the most commonly used modality in biology the alternative scanning transmission em stem has emerged as the mode of choice for atomic resolution in materials science seeking to endow biology with the benefits of stem our lab established stem for cryotomography of biological cells and demonstrated its advantages for thick specimens and compositional contrast we now seek to extend cryostem to highresolution with an emphasis on tomography by means of coherent detection obj1 this will be achieved by the method of integrated differential phase contrast idpc using a segmented detector from which we obtain simultaneously phase and depth contrast in a single scan the major expected benefits are 1 minimization of image aberration especially defocus with its associated complications for image interpretation 2 reduction of beaminduced radiation damage by means of flexible scan and sampling patterns and 3 improved reconstruction for tomography based on tailored data acquisition we will validate the new methods for single particle analysis on standard macromolecular substrates and compare them to current stateoftheart methods further we will apply the new developments in 3d imaging to explore novel largescale structures in chromatin we observed recently by wholecell cryostem tomography using current lowresolution methods obj2 labelling with halogenated nucleotides will reveal sites of active transcription or dna synthesis the proposed approaches expected broad applicability and stems unrealized potential for hardware simplicity should together ensure the wide adoption of cryostem methods in biology accelerated by our dissemination efforts obj3
quantumoptic silicon as a commodity extending the trust continuum till the edge of ict networks

the rapid rise of quantum computation has raised concerns about conventional encryption methods in response to this emerging threat quantum key distribution qkd offers an informationtheoretically secure key exchange however its complexity currently hinders its widespread adoptionqosilicious introduces a disruptive commodity approach integrating every element of quantum random number generation qrng and qkd monolithically on silicon this results in ultracompact implementations with clear photonicmicroelectronic cointegration strategy and excellent cost credentials allowing us to address new qkd markets a pixelsize 001 mm qrng will be realized in 018m cmos including a multidot silicon light emitting diode surrounded by a ringshaped singlephoton avalanche detector spad with cointegrated active quenching its true random numbers will feed a qkd transmitter developed on a siliconphotonic bicmos platform on a footprint of no more than 1 mm simplicity is ensured through a novel gesi light emitter sourcing a bb84 polarization state encoder this yields an allsilicon solution free from the burden of complex and costly iiiv silicon heterointegration the efforts on qosilicious qkd transmitter are complemented by surfacenormal gesi spad technology for 1550nm operation at detection efficiencies of 50 under peltier cooling the gesi spad will be transferred to the waveguidebased siliconphotonic platform to further yield an integrated qkd receiver including bb84 state analysisthe applicability of qosilicious technology to commodity segments will be evaluated in technoeconomic challenging scenarios i intradatacenter interconnects ii access and 6g networks and iii mobile applications with a closeproximity freespace optical key exchange performance evaluation activities will target securekey generation that enables the encryption of coexisting classical data traffic through fast aes key renewal
chemical tools for transcriptomewide analysis and modulation of rna

rna is an essential biomolecular polymer that exhibits many cellular functions ranging from catalytic to informational that are crucial for life long considered as dnas unexciting counterpart rna has in recent years transcended its dull image driven by pivotal discoveries of its involvement in cellular signalling pathways despite the clear importance of rna in biology and medicine many of its properties and functions remain elusivein the ribochem program we will develop groundbreaking chemical tools for uncovering the identity function structure and druggability of rna on an unparalleled transcriptomewide level with a special focus on riboswitches riboswitches are structured rna molecules that have gathered particular interest due to their involvement in bacterial gene regulation that render them attractive targets for antibiotic development the proposed tools will combine refined chemical design and synthesis tailored towards rnas chemical properties with advanced sequencing technologies this unique combination enables the discovery of riboswitches throughout the entire transcriptome using a photoaffinity labelling strategy customized chemical probes will be used to measure riboswitch conformational dynamics in response to environmental inputs and reveal how bacteria exploit these phenomena to finetune their gene expression finally a paradigmshifting strategy for modulation of riboswitch activity will be developed by applying small molecule covalent modulators of rna structural elements which has the potential of transforming the field of rna targeting taken together the research within ribochem will find broad applicability in the emerging field of rna chemistry and biology and help uncover many biological functions and properties of rna and yield important insight into rna targeting
very high energy electrons beam for radiotherapy

despite advanced treatment planning and delivery conventional oncologic radiotherapy rt is limited by acute toxicity and longterm side effects caused by radiation delivery to healthy tissues which often restricts the ability to deliver a sufficient dose of radiation to the tumours the problem is exacerbated when treating cancers that are either deep down inside the human body or in delicate areas where toxicity to nearby organs could cause serious side effects especially when treating obese patients whose numbers are increasing globally thus an accessible and costeffective alternative to current rt modalities is urgently needed in ercpoc project vherapy we made a breakthrough in demonstrating the viability of delivering highquality particle beams with compact laser plasma accelerators for very high energy electron radiotherapy vheert we demonstrated that such a vhee beam can be produced with a single laser beam using a new injection scheme making the acceleration machinery small simple and cost effective thereby giving this device of the future substantial competitive advantage the objectives of the ebeam4therapy project are to optimize vhee beam performances for radiotherapy applications further to characterise the 3d dose deposition to improve and scaledown each component of the machine and finally to build a machine prototype that demonstrates the technical feasibility in an economically competitive setting the commercial feasibility is validated with real market players and the project expands the existing network of collaborators and sources additional investment to accomplish the next steps in technological commercial development to speed up market entry ebeam4therapy aims to revolutionise cancer therapy by providing better clinical outcomes and radically improving the quality of life of cancer patients at a lower financial burden to payers and economies thus addressing a real and immediate global societal challenge
zerocarbon industrial heat production by ammonia water absorption heat transformer

decarbonizing industry is the key challenge for 2050 to achieve the net zero ambitions for this one of the current pathways is the massive electrification of industrial processes in demand for lowtemperature heat up to 150c thanks to heat pumps however the electricity distribution companies have recently issued an alert the grid development is insufficient to match the enormous future needs for electricity in the coming decades this will thus trigger tensions on the power grid and lead to priorization in the supply of electricity therefore to alleviate electricity demand while meeting industrial needs it is crucial to provide alternative technology such as absorption heat transformer aht which has the potential to meet the demand for low temperature heat from non critical and abundant energy sources such as industrial waste heat with hardly any electrical usewith this objective of offering another option than vapor compression heat pumps the zimba project aims to develop and validate at trl4 an innovative aht system at 15 kwth and 110c it will be based on a novel water ammonia aht technology improved by a twophase ejector specifically designed in order to stabilize its performance and widen the range of its operating conditions in particular under hot conditionsfrom the physical evaluation results obtained scaling studies up to 500 kwth will be carried out by the consortium composed of researchers and industrial manufacturers in order to push for a design with practically no critical raw materials easily maintainable and recyclable and with only commercially available components for fast time to market the integration of the system in the potential future energy market and the associated business models will be studied in order to ensure a circularity of the energy and to fully exploit the exergy of the heat lossesthus zimba will help the eu in reducing its dependence on fossil and electricity while meeting climate goals
ubiquitous ehealth solution for fracture orthopaedic rehabilitation

the population in europe is living longer and healthier and that is a great achievement on the other hand an ageing population raises major financial and social challenges one in four 25 persons living in europe could be aged 65 by 2050 the greater expectancy of life in europe is posing serious challenges to healthcare namely through the associated increasing incidence of various diseases as well as health conditions which the elderly are mostly prone one of the latter conditions is bone fractures which can typically occur as a consequence of osteoporosis furthermore the consequences of associated complications in fracture recovering include further costs not only for the patient but also for the european society in general to address such growing issue the multidisciplinary consortium of robust takes this as a challenging use case for demonstrating the relevance and proficiency of smart mobile ehealth systems as innovative solutions to address surging issues in our ageing societyrobust targets developing a new concept and platform for remote monitoring of patients healing process in the ehealth domain an integrated mobile ehealth system will be devised exploiting recent advances in rfbased sensing technologies which are being investigated in this consortium the system will be able to respond promptly to dynamic and complex situations while preserving control safety and privacy in a reliable and energy efficiency manner the robust system will include a fast feedback loop that dynamically processes sensing information to generate accordingly instructions to the patient encompassing cognitive and learning capabilities as well robust is committed to create an exceptional network which is multidisciplinary and intersectoral in nature for staff exchange in the mobile ehealth field namely targeting structure training and knowledge sharing towards enhancing the european innovation capacity in relevant ehealth systems and applications
agripv system with climate water and light spectrum control for safe healthier and improved crops production

pv4plants boosts the energyagricultural synergy of agripv technologies to enhance growth conditions and increase landuse efficiency crop yield and renewable energy generation this is achieved by optimising the light transmission of pv panels through cutting edge nanoparticles spraying on the pv glass surface pv4plants system is specifically designed to meet healthy harvesting and to be adaptable to different climatic conditions and crop varieties that will be demonstrated in 3 highly replicable demo sites in turkey spain and denmark pv4plants optimises the system parameters panels tilt irrigation level amount of fertilisers etc based on a multiindicator realtime monitoring system that allows a continuous improvement of the microclimate underneath the agripv panels to increase crop health and yield recyclability and reutilization of components and materials both for the manufacturing and end of life of the pv4plants system are central aspects of the project the pv4plants system will be certificated through the environmental product declaration epd compliance with iso 14021 and the sustainability excellence label by unef finally pv4plants will boost its market penetration and uptake through innovative farmers engagement strategies to enhance their acceptance and trust in innovative agripv systems new financing schemes and business models to improve investment performance and through a set of policy recommendations that will be developed together with public authorities to create new mechanism designed to accelerate the uptake of agripv systems in europe
governing quakerty in a postdisaster türkiye

this academic research entitled governing quakerty focuses on the intersection of quake and property and sheds light on property governance in a postdisaster context particularly in and around the recently quakeaffected area of antakya in türkiye where the research is focused the central research question is how does property governance unfold in the earthquakeaffected city and what are the governmental communal and individual strategies implemented to deal with land and property loss appropriation and the ensuing processes of reentitlement during the reconstruction period the research examines the dynamics of disaster property in a postquake context encompassing both the collapse the process of alienation and reconstruction the process of reentitlement phases by investigating a the disposal recycling and governance of earthquakedamaged property and personal belongings within collapsed or damaged buildings b the process of postdisaster property claims and strategies for reentitlement and ownership during relief and reconstruction efforts by examining the circulation of property along the processes of loss recycling reconstruction and reentitlement in the context of a destroyed urban landscape in conjunction with legal and institutional contexts and policies as well as the strategies and experiences of affected individuals this scholarly investigation will make a critical contribution to the development of an effective approach to property governance in quakeaffected areas thereby assisting resilient and inclusive postdisaster property recovery efforts
aibased learning for physical simulation

computational physical modeling is a key resource to complement theoretical and experimental methods in modern scientific research and engineering while access to large amount of data has favored the use of artificial intelligence and machine learning ml techniques to enhance physical simulations limitations of purely datadriven methods have emerged as concerns their generalization capability and their intelligibility in particular the latter feature promotes understanding a fundamental driver for scientific and technical progress and possibly allows to rigorously investigate the reliability of the models and the safety of the systems based on those models to overcome these limitations i propose a hybrid approach that originally combines ml methods and equationbased modeling to significantly improve generalization in smalldata scenarios while guaranteeing the intelligibility of the physical models through the use of symbolic representations core of the methodology are learning algorithms that reconstruct models with controllable complexity from data by consistently combining building blocks which derive from a unifying mathematical framework for physical theories the system will also incorporate novel humaninspired strategies for knowledge distillation accumulation and reuse which are missing in stateoftheart physical model learning algorithms to efficiently handle the computational cost associated with the proposed methods i will implement them in a new software platform that seamlessly integrates automated model learning and highperformance simulation thanks to their generalpurpose nature the methods and algorithms developed in this project may be employed in all scientific disciplines and in engineering workflows in particular i plan to use them to advance biology and soft robotics by solving challenging modeling tasks
tackling micronutrient malnutrition and hidden hunger to improve health in the eu

micronutrient mn deficiency is a form of malnutrition that occurs due to low intake andor absorption of minerals and vitamins with adverse consequences for human development and health children adolescents women of reproductive age including pregnancy and older adults representing 70 of european society as well as immigrantethnic minority groups and those affected by social inequality or poverty are at risk of mn deficiencies while addressing this public health problem is a priority it is not possible until data on the prevalence of mn deficiencies across the eu population and the causes for these deficiencies is available to predict and identify those most at risk without this information discussions on how to meet dietary requirements for the priority mns of public health concern and improve their status take place in a vacuum the zerohiddenhungereu project aims to 1 provide estimates of the true prevalence of mn deficiencies based on priority biomarker and mn intake data in european populations and their associated health costs focused on highrisk population subgroups and 2 provide the best possible evidence to develop contextspecific tailored foodfocused solutions to ensure adequate supply of vitamins and minerals from diets from sustainable sources the consortium will focus the research effort around existing highquality data resources and biobanks from diverse and representative population groups around europe supplemented by targeted studies in underrepresented groups maximizing research investment and adding value using highthroughput biomarker and advanced data analytical and modelling techniques zerohiddenhungereu will deliver credible evidence enabling policymakers and food system actors to deliver foodfocused strategies to eradicate mn deficiencies from europe