Dragon09/odtu_chatbot huggingface dataset:
decoding enhancer usage and gene expression of human heart development in 3d cardioids

the early steps of heart formation during embryogenesis require precise spatiotemporal coordination between different heart progenitors to join together to form a beating heart tube this complex biological process is regulated through an evolutionary conserved gene regulatory network grn of transcription factors and cisregulatory elements that integrate inductive signals from surrounding tissues to ensure correct heart development yet how this process is controlled during heart development and how mutations impacting the cardiac grn leads to cardiomyopathies remains unclear there is currently little or no information about human heart enhancers during these early stages of heart development here we will first determine the dynamic changes in the usage of regulatory elements and gene expression that accompanies human heart development using heart organoids cardioids derived from human induced pluripotent stem cells to uncover the underlying grn we will apply scrnaseq and scatacseq to 3d cardioids at different stages of development which will allow us to map enhancer usage during cell fate transitions in human heart development for the first time in vitro moreover we will study how developmental trajectories are altered in diseaserelevant mutant 3d cardioids models last we will study how perturbations of transcription factors influence enhancer function and gene expression by finetuning the nuclear level of key regulators using an optogenetic tool developed by the furlong lab which is extremely dynamic providing very precise temporal resolution in the order of minutes the functional impact of these perturbations will be assessed by scrnaseq and scatacseq to identify the transcription factors regulatory mechanisms together these findings will advance our understanding of gene regulation during cardiogenesis by identifying fundamental principles of enhancer usage and transcriptional changes during normal and diseased heart development
gender gaps in innovation from entrepreneurship to academic science

innovation is widely recognized as a key driver of economic growth however large gender disparities persist in crucial components of the innovation ecosystem entrepreneurship and academic science in the eu for instance women represent only 34 of entrepreneurs globally femaleowned businesses tend to be smaller less profitable and face challenges raising external funding this disparity also extends to academic innovation where women remain underrepresented and are less likely to receive credit for their research moreover critical research areas such as health issues that predominantly affect women remain understudied given the implications for economic growth and societal wellbeing the overarching goal of this research is to understand the determinants of the gender gaps in entrepreneurship and science as well as their consequences for innovationto address these knowledge gaps during my msca fellowship at tilburg university my research objectives will 1 explore potential determinants of the gender gap in entrepreneurship 2 investigate interventions and policies that can address these gaps and 3 understand the consequences of the lack of gender diversity in innovative activities especially in academic science on the production of ideas and how ideas by women are perceived to achieve these objectives i will conduct three interconnected studies in the first study i will use dutch microdata to assess the role of motherhood and the impacts of maternity leave policies on femaleowned businesses second i build upon an experiment in ghana to evaluate how access to online networking can close the gender gap in business performance finally i will investigate how increasing gender diversity at universities can impact research topics and the recognition of femaleauthored work together these investigations delve into gender disparities in entrepreneurship and refine our understanding of how diversity fosters innovation
ultralow designeffort energyefficient and batteryindifferent sensor node for the green internet of things

the rapid technological improvement in the semiconductor industry has enabled smaller and smaller devices to pervade our everyday lives the real challenge in reducing the size to facilitate a ubiquitous integration of smart functions in any thing is drastically reducing the power consumption that constrains the volume to the presence of bulky batteries an electronic device that dramatically reduces its power consumption can be powered by an alternative green source of energy such as light or vibration enabling the vision of the internet of thingsin this scenario the proposed research activity is intended to substantially enhance the energy efficiency of an iot sensor node by a synergetic approach targeting both multisource harvesters and the systemonchip soc design the latter one aims in a comprehensive approach in which the macroblocks of different nature are designed exploiting at most an automated digital design flowon this basis a dramatic humandesign effort reduction and silicon area for the same operation is expected for any soc by a factor in the range of 10x100x this implies a faster timetomarket and a reduced cost of energyautonomous wireless sensor nodes challenging problems to be addressed are performance ie resolution speed the effectiveness of the proposed approach will be verified and validated on demonstrators of industrial interest the project targets to be innovative interdisciplinary and intersectoral across academies an eubased semiconductor company and partners from ms and tc mostly based in the asen
taphart exploring the potential existence of rock art paintings in the middle stone age of southern africa and its destruction due to the action of taphonomic processes

the advent of symbolic material culture and the storage of information outside the human brain through the production of artefacts and images imbued with meaning was a landmark in human evolution although it has been generally considered as the consequence of an european upper palaeolithic revolution that took place around 40 ka new evidence from the middle stone age msa 30040 ka suggests the presence of symbolic material culture in africa at 150 ka in this context a major research challenge for archaeology is to explain the apparent absence of rock art paintings in the msa archaeological record of southern africa ochre pigments are ubiquitous in the sites from this period and toolkits used to produce ochrerich liquid paint date from at least 100 ka furthermore images engraved on pieces of ochre and a crayon drawing demonstrate that the msa populations had all the material technical and cognitive resources necessary to create graphic representations thus through an innovative and interdisciplinary approach that brings together archaeology paleoenvironmental science atmospheric science material science and analytical chemistry the taphart project aims to develop an experimental research to determine whether the absence of rock art paintings in the msa sites of southern africa is due to a cultural choice of the human populations or the result of taphonomic processes that eliminated the painted images from the archaeological record taking as reference the archaeological and paleoenvironmental context of blombos cave south africa the project tests the hypothesis that marine aerosol and wind erosion may have contributed to the destruction of rock art paintings potentially produced on the site providing groundbreaking unprecedented data to the discussion on the origins of rock art and the behavioural evolution of the homo sapiens
advancing the development and implementation of hologenomics in biological sciences

basic biology as well as applied fields such as agrobiology biomedicine or biotechnology are undergoing a revolution driven by the realisation that microorganisms impact virtually all biological processes microorganisms associated with complex organisms are no longer considered passive passengers but active crews that continuously interact with their host to shape a wide range of biological functions that play a key role in major basic and applied processes the application of the newest high throughput molecular technologies to address such interplay has given rise to the novel field of hologenomics which entails the joint analysis of host genomes and microbial metagenomes with the aim of understanding the impact of hostmicrobiota interactions in basic and applied biological processes the dn hologen has been conceived to lead the development and practical implementation of hologenomics in order to gain a more comprehensive understanding of hostmicrobiota interactions and their impact in both basic and applied areas with direct relevance to many of the global societal challenges we propose to form an unprecedented multidisciplinary and scientifically sound training network by bringing together top researchers with theoretical knowledge on symbiosis biology microbial ecology animal evolution animal production and biomedicine as well as practical expertise in large scale metagenomics dna sequence analysis metabolomics computational biology and biological systems modelling we anticipate that the research results will be i of academic relevance through development of new analytical frameworks and understandings of hostmicrobiome interactions ii of direct relevance to the portfolios of the industrial partners and iii through these portfolios stand to benefit the european research and innovation community
european researchers night and researchers at schools israel 20242025

the public nationwide especially youngsters will meet researchers and experience research inside and outside campuses peripheral and central cities arabicspeaking and hebrewspeaking communities informal festivity settings and schools it will allow discussions at eye level between researchers and kids teens and adults alike discussions about their common hobbies accomplishments curiosity and the science in between the public loves it and the researchers love it measured over our ernis since 2005we have a welltrained consortium running since 2005 operating a very appreciated erni project with an excellent reputation we have the ministry of innovation science and technology support in funding the awareness campaign and in professional personnel as well as full involvement of the eu embassy in tel aviv
urban planning and design ready for 2030

up2030 aims to guide cities through the sociotechnical transitions required to meet their climate neutrality ambitions it will do so by enabling a quantum leap from a business as usual projectbyproject decarbonisation approach to a visiondriven strategybased approach that is anchored on sound projects and renewed policy development the approach uses urban planning and design as a vehicle to create better connected more compact netzero neighbourhoods in the city pilots ie neighbourhoods that promote liveability and through designing with intent promote mitigation action unlike fragmented innovation processes that focus on the deployment of a specific solution to achieve incremental progress up2030 proposes that cities should themselves be at the centre of the innovation approach to drive transformative change the project develops the 5up methodological framework that supports cities in i updating those policies codes regulations that need to be left behind to make room for the new vision ii upskilling through building the capacities of the entire city stakeholder ecosystem that shall deliver actions iii upgrading through the development of solution prototypes digital and physical at selected neighbourhoods iv upscaling to achieve citywide impact by shaping the enabling governance arrangements and matching project portfolios to financial resources and v uptaking by engaging with the mission and sharing best practices across european cities inclusive participation is key throughout the projects full cycle of activities so that real needs of communities are reflected in the cityspecific visions and codesigned interventions maximise delivery of cobenefits as such up2030 will a have a measured positive impact on spatial justice in the pilots and b give the opportunity to citizens to participate in the transition by becoming agents of change themselves through their sustainable behavioural shifts
a novel framework for contactless diagnosis and forecasting of cardiovascular diseases

in ancient times doctors have followed unorganized practices to diagnose cardiac arrest conditions of healthcare patients the followed medical procedures were not very organized and accurate even though cardiac patients were diagnosed using devices such as a stethoscope however the latest advancements in the technologies such as contactless remote patient monitoring aiomt artificial intelligence of medical things advanced big data and cloudbased analytics and alerts have created a paradigm shift in healthcare and provided 24 x 7 connectivity the study proposed a vcardiac contactless cardiac classification and risk prediction framework to diagnose and identify early stages of heart diseases and forecast their cardiac conditions in advance using the human voice in this study a customized dataset termed cardiac2000 with 2000 human voice samples will be designed to classify acoustic heartbeat events such as normal murmur extra systole artifact and other unlabeled heartbeat acoustic events the average duration of the recorded heartbeat acoustic events would be 10 to 12 seconds the primary reason for designing a customized dataset cardiac2000 is to balance the total number of samples into categories such as normal and abnormal heartbeat acoustic events for performance evaluations of the proposed vcardiac framework the collected heartbeat acoustic samples will be classified using lstmcnn rnn lstm bilstm cnn kmeans clustering and svm methodologies furthermore the proposed vcardiac framework will also assist in classifying age and genderwise risks using methodologies such as kaplanmeier and coxregression survival analysis these methodologies will also assist in identifying the probability risk and the 10year risk score prediction in the end the proposed vcardiac framework will be tested for various signal to noise ratio conditions for achieving better accuracy effectiveness and throughput
the malaria zoo dissecting cerebral malaria in three in vitro primate bloodbrain barrier models

plasmodium falciparum cerebral malaria cm is the most severe complication during malaria infections cm is associated with patient coma and bloodbrain barrier bbb disruption resulting in brain swelling and patient death in the zoonotic malaria parasite p knowlesi cm is described in rhesus macaques but occurs neither in human infections nor in the natural longtail macaque reservoir infected red blood cell cytoadhesion to endothelial cells and the release of parasite toxins have been described as pathogenic mechanisms of falciparum cm both factors are present in p knowlesi in all three primate hosts albeit at different levels and result in different clinical outcomes therefore i hypothesize that the pathogenic mechanisms of cm in primates are host and parasitespecific as in vivo studies are limited to post mortem samples i aim to develop in malzoo novel microvascular 3d in vitro models of the human rhesus macaque and long tail macaque bbb i will adapt existing differentiation protocols for human induced pluripotent stem cells ipsc to generate macaque endothelial cells astrocytes and pericytes that will be introduced in a 3d microvascular model taking advantage of the microfluidic properties of the devices i will characterize the dynamics of p knowlesi cytoadhesion to the endothelium of the three hosts afterwards i will measure the bbb pathogenicity of these two parasites by confocal microscopy functional permeability assays and rnaseq transcriptional analysis and correlate it to the parasite cytoadhesion levels the crossspecies comparison will be used to highlight speciesspecific virulence factors during p falciparum and p knowlesi infections this will improve our understanding of the mechanisms leading to p falciparum cm in humans and the pathogenic potential of zoonotic p knowlesi malaria
thermal modulators based on novel 2d mxene materials for nearly isothermal battery operation

batteries represent the present and future of our electrified society one of the challenges within this field is ineffective battery thermal management btm leading to device failure limited performance and lifetime reduction btm require a effective dissipation of heat in warm environments to avoid thermal runaways while b the retention of heat in cold environments to avoid energy drops this relates to the inevitable compromise of cooling dissipate heat and insulating retain internal heat in batteries depending on the needs current btm are too bulky and provide mostly cooling capabilities hampering batteries to perform optimally solidstate thermal modulators represent the ultimate solution for regulating batteries temperature however their performances and sizes are far from sufficient to deploy them for btmthermo2deal aims to develop a novel interfacial thermal modulator that enables dynamic heat management in batteries to achieve nearly isothermal performance we will develop specific types of large area 2d transitional metal carbides materials ie mxenes that will be tuned electrochemically to modulate their thermal properties these mxenes will be the essence of a new thermal modulator design for facile integration in batteries the scalability its quickly and repeatedly toggle on and off and its large hot to cold switching contrast makes it pioneer in the field i will address scientific challenges in the synthesis of scalable and unique mxenes demonstration of thermal tuning on them and their integration in pouch cellseg basic battery stack units in cars or house appliancesfor temperature modulation experiments and theory using a nano to macroscale approach will be used to overcome these challengesthis proposal will be a key stepping stone in developing advanced btm for improved battery performance and lifetime in the long term this project will be at the frontier of new thermal technology for energy recovery or storage
custommade designer macrophages to revolutionize the safety of parenteral drugs

the ipyro proposal is built upon the uniqueness of human induced pluripotent stem cells ipsc and recent innovations in the farming of fully defined human macrophages imonomac using industry compatible bioreactors given the growing need for industrial macrophages ipyro sets an innovation in the forefront of drug safety evaluations supplying a unique cell type which meets the expectations from industry and regulators the breakthrough innovation of ipyro will offer a superior thus highly standardized economically produced and fully validated offtheshelf macrophagebased cell product to revolutionize the safety of drugs and beyond to maximize the outcome ipyro will assess the regulatory compliance while further streamlining the imonomac product for the envisioned spinout establishing a proper freedomtooperate strategy and a suitable business model the seminal imonomac platform along the unique ipr and established contacts to industrial and regulatory stakeholders will pave the transition from academia to industry introducing for the very first time an ipsccell product for the drug safety market market
training network for exploring microalgae diversity for the development of novel antiviral compounds

the algaenet4av project seeks to exploit the microalgae biodiversity as a source for highaddedvalue lectins and polyphenols with antiviral properties against influenza and coronavirus pandemic strains microalgae derivedcompounds are easily amendable biodegradable nontoxic and safe thus they are preferred to be used for the development of novel biobased materials and cosmetics with antiviral potential lectins and polyphenols could be highly demanded nondrug interventions across the materials and cosmetics industry as a solution to reduce the risk and speed of contamination and transmission during pandemic outbreaks the project will combine the whole value chain in order to a develop transcriptomics and metabolomics resources from microalgae diversity as a valuable source for the discovery novel lectins and polyphenols b characterize their spectrum of bioactivity and toxicity and provide structurefunction relationships c develop and optimize applicationbased microalgae culture systems and downstream processing strategies for higher production rate of the desired compounds d develop formulate and evaluate lectins and polyphenolsbased final cosmetics products the work will be supported by market assessment integral biorefinery designs technoeconomic and sustainability assessment integrated value chains will be demonstrated to deliver proofofconcept and demonstrate economic feasibility algaenet4av is an industryacademia rd and innovation project with a multidisciplinary approach aimed at generating robust business cases through technology development the consortium composed by 11 partners with 7 european and 2 thirdcountry usa japan prominent research organisations strong industrial leadership is guaranteed through the participation of 5 smes the projects goals will be achieved through a sustainable way helping meet the increasing demand while creating a positive environmental impact through reduction of carbon emissions
driving inclusive care economic democracy and social economy

social economy organisations play a crucial role in tackling social exclusion however the development of social economy governance frameworks and their impact on social inclusion remains insufficiently explored the dices project aims to bridge this gap with a threefold objective i enhance understanding of europes social economy and identify factors driving its uneven development ii evaluate the role of social economy organisations in addressing social exclusion and improving services and workplaces iii recommend policy measures and governance frameworks to unlock the social economys potential including strategies for identifying and promoting best practices dices first establishes a conceptual and empirical framework and conducts a mapping examining the social economys integration into welfare governance frameworks second dices uses deep dives to investigate contributions at local including in left behind places and organisational levels including in the provision of care this work builds on case studies and pilot actions a survey smallscale experiments and other innovative methods this approach is connected through the identification of conditions that would allow organisational experiments to scale up into institutional experiments dices uses interdisciplinary methods across eu and noneu countries the project engages stakeholders through codesign of pilot actions and peer review cycles complementing quantitative and qualitative studies stakeholder input helps develop concrete policy proposals dices disseminates findings through research papers policy briefs a digital storyboard webinars and toolboxes for policymakers and practitioners to maximise impact on policymakers stakeholders and the public