Dragon09/odtu_chatbot huggingface dataset:
versatile amplification method for singlemolecule detection in liquid biopsy

robust detection of single molecules in complex biological fluids is the ultimate goal in the field of disease biomarker analysis conventionally to enable the quantitative analysis of individual molecules in macroscopic volumes analyte preconcentration and sample partitioning into flnl compartments has been combined with the amplification of the specific recognition events in these setups the positive or negative detection of fluorescence signal is triggered by enzymatic reactions occurring in each compartment binary readout based on poisson statistics quantifies ultralow concentrations of analyte molecules this approach has been adopted for nucleic acids analysis in current digital pcr and is also available for proteins in a technique coined as digital elisathe objective of versilib is to develop an enzymefree amplification strategy for the analysis of both protein and nucleic acid analytes with the single digital platform that offers means to access additional information on target analytes not achievable with current technologies method is based on novel affinitymediatedtransport amplification where affinity interaction of target analyte with a specific ligand attached to a magnetic nanoparticle transporter is accompanied with rapid shuttling of fluorescent tracers that serve as reporters by applying external magnetic field tracers are transported from the tracer storage side where they are dark to tracer active side where they become bright only if target analyte is present in the small reaction compartment tailored plasmonic nanostructures will be prepared at the storage and active sides of the compartment to render the tracer either dark or bright the aim is to perform technology validation for the novel versilib proteogenomics amplification platform in cancer management using biobanked liquid biopsy samples
centre of excellence for safety research

the use of hydrogen and other alternative energy sources as a clean and sustainable source have been gaining momentum worldwide due to their potential of reducing greenhouse gas emissions and supporting the transition to a lowcarbon economy to map analyze and understand the safety risks of the widespread deployment of hydrogen and other alternative energy technologies a new multidisciplinary research group has been established at the faculty of safety engineering fse vsbtechnical university of ostrava vsbtuo to reflect the requirements and needs of industry public authorities and other stakeholders to help them establish a safe environment promote public acceptance of these technologies and build a critical mass of knowledge for their further development safeenergy project will use scientific potential in the field of hydrogen and alternative energy sources safety and support fse in international networking projects and other initiatives on an international level this will be achieved through the leadership of the era chair holder experienced researcher and manager prof salzano from the university of bologna creation of a pocket of excellence centre for research on the safety of alternative energy sources and through building capacity in the field of research management and administration at the faculty of safety engineering vsbtechnical university of ostrava scientific collaboration will be established and developed through networking activities and joint research with industrial partners and international partners from europe and worldwide to increase excellence in energy safety with a focus on hydrogen cities and valleys the project will especially support young scientists postdocs and phd students to step up and stimulate scientific excellence and innovation capacity in hydrogen and alternative energy infrastructure safety the project proposes a comprehensive set of activities
cinematic atmospheres towards a new ecology of the moving image

cinema is not just a representational but also an operational medium its global success is indisputably tied to its ability to embed its audience in affectively charged atmospheric worlds and filmmakers carefully design their narrative worlds through ambient soundscapes music colorations filters camera movements production design mise en scne rhythmical editing and countless other features that arrange the emotional and affective experiences of the audience marginalized in film studies due to their ontologically indeterminate nature cinematic atmospheres operate affectively and prior to cognitive critical and analytical reflection this project examines cinematic atmosphere production in all its experiential aesthetic and ethical implications as an attractive but also problematic even harmful and ideologically loaded enterprise it raises critical awareness of the basic atmospheric operations of cinema in a time when the medium is relocated into new media environments and its techniques are reused within ecologies that are not cinematic per se such as news broadcasting videoconferencing sports refereeing and even terrorist recruitment this project i develops a broad conceptual understanding of cinematic atmospheres as a way of organizing the perceptive affective and cognitive epistemic access to the world of the film and ii mobilizes this understanding to develop a method for the study of the operations and techniques that constitute cinematic atmosphere production before iii expanding this method to deal with the question of how these cinematic operations and techniques for atmosphere production are no longer the property of cinema alone but increasingly employed outside of the narrativeaesthetic domain at the service of larger sociocultural economic and political forms of organizing perception cognition and affect from art installations to social media
a prosthetic heart valve for adults and children made from the patients own tissue a replacement heart valve that lasts a lifetime

each year 400000 people are born with heart valve defects worldwide moreover in the western world 1 in 8 healthy adults will develop a heart valve problem during their lifetime current artificial heart valves made from animal or human donor tissue carry a high risk of tissue rejection degradation and calcification that limit their lifespan to about 10 years forcing patients to undergo risky cardiac resurgery for children no dedicated valve product exists thus paediatric patients can endure 45 surgeries between 018 years of age as their bodies outgrow each replacement heart valve at grownvalve we have developed a paradigm shift in heart valve technology the first autologous valve grown from the patients own tissue crafted using medical imaging and a 3d printing technique our valve lasts a lifetime eliminating tissue rejection and surgical risks associated with repeated surgery bleeding infection and death
women building brazil a history of architecture from the perspective of selfbuilding sites

women building brazil wbb seeks to underscore womens contributions to architecture and construction in brazil particularly in contexts of selfbuilding its main objective is to address the neglect of both women and informal building practices in architectural and construction history especially in the global south the project will tackle that double historical gap by gathering personal stories and documentation on women builders its premise is that womens involvement in selfconstruction was actually quite common in vernacular architecture but has been affected by the introduction of new building technologies and materials expanding our disciplinary scope to include vernacular contexts wbb employs a novel method combining oral history with disruptive archival research methods and communitybased participatory practices guided by feminist and decolonial principles this approach will be applied in three territories in brazil where selfbuilding remains a prevalent custom quilombos afrobrazilian maroon communities indigenous territories and favelas urban informal settlements these distinct settings offer new insights to help recover analyse and acknowledge womens construction knowhow the outcomes of wbb include an oral history archive and a public video collection the participatory process will also generate a memory artefact cocreated with interviewed women with the purpose of recognizing their expertise and strengthening their building autonomy in summary wbb offers a new methodological approach to a growing field of construction history and a gendered analysis that contributes to the broader discipline of architectural history it maintains a commitment to open science through its research methodology and participatory practices ensuing constant dissemination of its results most importantly wbb actively seeks ways of empowering women in construction giving them voice and reaffirming the relevance of their knowhow
gaming clusters across multiple european regions

the main objective of the project gaming clusters across multiple european regions gameer is to research how video games clusters emerge develop and sustain themselves with particular focus on local and regional clusters based on this research gameer will formulate a series of policy and practical recommendations which will compose an interactive methodological toolkit these recommendations are aimed especially at local and national policy and decisionmakers with the intent to help them bootstrap cultural and creative industries clusters or strengthen existing clusters in their regions or citiesmuch of existing research on video game clusters concentrate on clusters outside europe or on european clusters in capital or large metropolitan cities such as helsinki or hamburg however local and regional clusters also are key drivers of innovation growth and cohesion and gameer will fill the gap around the limited knowledge we have about them to achieve its ambitious objective gameer a core component will be a comparative analysis of six local and regional clusters in five european countries which include france the czech republic italy scotland and portugal these clusters have been selected because of their different levels of maturity and their specificities such as the concentration of human creativity or companies moreover gameer will conduct a europe wide analysis of the spatial organization of the european video games industryecosystem with focus on local and regional clusters through this research the project will formulate policy and practical recommendations using a participatory approach and working with policy makers and the industry the project is organised in six work packages with 3 dedicated to research clusters and one focusing on building the project toolkit the project includes 16 partners from 9 countries with expertise in social sciences and humanities policy making the thirdsector business and innovation
reinventing highperformance power converters for heavyduty electric transport

rhodas project aims at developing disruptive topologies of power converters using new semiconductor materials as well as cuttingedge digital technologies to improve architecture efficiency power density reliability cost and sustainability moreover multidisciplinary approaches of modular power electronics for integrated motor drive imd and ecodesign considerations are addressed to create compact solutions that can be integrated in a wide range and heavyduty vehicles enabling these electric vehicles to be more sustainable and autonomous throughout the entire lifecycle of their components nevertheless power electronics solutions that use wide band gap wbg devices can also be applied to lightduty vehicle types m and l with competitive advantages on the efficiency and power densities compared with current technologies finally the rhodas project targets the validation of the proposed solutions in electric drivetrains of 1200v for zero emissions class n3 carriage of goods 12 tonnes and o4 trailers 10 tonnes which correspond to usa class 78 heavy duty vehicles 12 tonnes and beyond
highthroughput screening synthesis and characterization of active materials for flow batteries

predictor aims to establish a rapid highthroughput method to identify and develop materials for electrochemical energy storage this method will comprisea modelling and simulation tool for the computational screening of organic chemicals based on their potential performance in energy storage systemsautomated chemical synthesis electrolyte production and characterization methods so that the chemicals identified in the screening step can be rapidly produced and tested for their suitability in energy storage applicationsartificialintelligencebased selfoptimization methods that allow experimental data from material characterization to be fed back into automated experimental methods to enable selfdriving laboratory laboratory platforms and for modelling and simulation tools improving their accuracydata management systems to standardize and store the data generated for further use in model validation and selfoptimization proceduresthis approach will allow the rapid identification synthesis and characterization of materials within a coherent development chain replacing conventional trialanderror developments it will exploit the synergies between several emerging markets digital technologies artificial intelligence highthroughput experimentation renewable energy storage providing the recruited doctoral candidates dcs with a valuable interdisciplinary skill set to validate the predictor system the case study will be active materials and electrolytes for redoxflow batteries within the project three demonstrator battery cells trl34 will be assembled and tested with the newly developed materials
emergent awareness from minimal collectives

awareness in biological agents has converging definitions when considering local states describing contentrelated consciousness from an agentspecific perspective however it becomes highly debated when it comes to global states the issue magnifies when considering collectives of artificial agents several frameworks exist all unsatisfactory in the limitations posed to agents heterogeneity and disappearance of the local self into an integrated state ultimately existing frameworks are ineffective in explaining facilitating and supporting cooperative behaviours in artificial agents the lack of a compelling theory of global awareness in ai is currently a significant barrier to the effective deployment of artificial agents in the real world emerge tackles this grand challenge by introducing the novel concept of collaborative awareness for collectives of minimal artificial beings we will investigate how simple agents can develop a representation of their mutual existence environment and cooperative behaviour towards the realization of tasks and goals emerge builds on a scenario of artificial beings with no shared language and constrained individual capabilities which nevertheless leads to highcomplexity behaviours at the collective level collaborative awareness becomes an emergent process supporting complex distributed and loosely coupled systems capable of high degrees of collaboration selfregulation and interoperability without predefined protocols emerge delivers a philosophical mathematical and technological framework that enables us to know how and where to allocate awareness to achieve a goal through the collective optimally we will demonstrate emerge concepts on robotic use cases with hints of broader applicability of the framework to internet of things pervasive computing nanotechnologies we will also investigate the ethical implications of collaborative awareness focusing on moral responsibility vulnerabilities and trust
democracyinaction grassroots culture arts and cultural spaces for political participation and expression online and offline in a resilient europe

democracyinaction studies arts and culturebased grassroots organizations in physical spaces and their social media and metaverse dimensions to understand their critical potential in fostering democracy democracyinaction identifies solutions for increasing meaningful political expression and participation in four key areas of grassroots social transformation of untapped potential 1 nightivism urban nighttime cultural life for political engagement
boosting the reduction of the environmental impact of pharmaceutical products throughout their entire life cycle

eternal aims to contribute to sustainable development of pharmaceutical manufacture use and disposal by using and promoting full life cycle approaches covering design manufacture usage and disposal assessing the environmental risks of not only the api and residuesmetabolites but other chemicals and byproducts of the production process this type of approach is essential to take into consideration the types of green manufacturing approach under consideration by the pharmaceutical industry as evidenced by the range and scope of case studies being undertaken within eternal specific application of our risk and life cycle assessment approaches to the eternal case studies is a key element of the proposed work and will provide industry and policymakers with key examples of how whole life cycle assessment may be used to evaluate the changes in environmental impacts expected due to the introduction of green manufacturing processes
from the margins to the masses standard practices and innovative uses of technology in augmenting different abilities of people in the world of work

newworktech engages in researchbased enhancement of the workrelated capacities of a people with disabilities and b the general workforce with particular focus on technologically mediated tasks and interaction the project covers the entire arc of development from empirical research into how people with disabilities as forerunners of technology use perform tasks and interact at work to theoretical innovation regarding the nature of sociomaterial assemblages as well as what constitutes technology to policy recommendations and to the development of new technological solutions including aibased technologies we consider people with disabilities to be experts in using technical systems to improve their work performance and through an analysis of a variety of use cases will identify elements of successful use as well as factors that cause problems for interaction or the performance of tasks empirical results will also be generated by surveys and cocreation activities people with disabilities will be engaged in newworktech in line with the principle nothing about us without us ngo partners will be actively involved as facilitators in the empirical research and as drivers of application and dissemination through our own technological development effort attention to the ethical use of technologies and the dissemination of the knowledge we generate the results will feed into improved usage methods and functionality of workrelated technologies as well as knowledge about the required skills the smart use of technology thus achieved will help create employment opportunities for people with disabilities improving their quality of life and reducing inequalities while also helping alleviate the current shortage of labour in europe and open possibilities for more efficient and creative use of technology by everyone whether they have physical or cognitive limitations or not
deciphering the interplay between stem cell differentiation and stem cell dynamics in branching morphogenesis

tissue development requires the orchestration of stem cell differentiation and morphogenesis a process that is conspicuously observed in mammary gland mg branching the mg develops from an embryonic bud of mammary stem cells mascs although these stem cells are initially bipotent giving rise to the outer basal and inner luminal layers of the adult gland they become lineage restricted very early in embryogenesis at around the time of the onset of branching morphogenesis the timing of lineage restriction coincides with the differential expression of cell polarity and cell adhesion transcripts branching morphogenesis then relies on differential cell division as well as cell rearrangements and migration but the mechanisms regulating this process in the embryo remain poorly understood based on these observations we postulate that basalbiased and luminalbiased mascs have distinct molecular features during branching morphogenesis we therefore propose to functionally link the signaling pathways involved in restriction of stem cell potency to the cellular dynamics that ensure branching to this end we will characterize the dynamic behaviour of individually labeled mascs during the growth of embryonic mg explants and assess their fate creating a complete fatebehavior map of mg development through genetic manipulation we will probe how cell adhesion migration and division impact on lineage specification and tissue growth simultaneously we will skew stem cell differentiation trajectories and assess their influence on cell behavior and organ shape the outcomes of this project will provide novel insights into the coordinated developmental programs governing stem cell fate and morphogenetic events both essential to tissue formation moreover they might uncover potential connections to cancer where differentiatiom and cell polarity play pivotal roles
safe and sustainable by design graphenemxenes hybrids

the safari project aims to develop new 2d materials using sustainable and safe processes the project focuses on creating hybrid formulations of mxenes and graphene gr which are known to possess unique and desirable properties such as thermal stability electrical conductivity the goal of the project is to develop sustainable and safe materials that can be used in a wide range of applications such as biosensors conductive ink and emi shielding the safari project begins with the preparation of precursor compounds known as max phases these compounds are then used to produce two types of mxenes ti3c2 and cr2c which are further functionalized to enhance their properties and increase their affinity with graphene the resulting 2d hybrid materials are created using two different methods and their structural morphological and functional properties are thoroughly examined one of the main strengths of the safari project is that is allinged with the ssbd principles thus assessment of the toxicological and ecotoxicological profiles of the new materials through a range of tests and assays will be conducted in conclusion the safari project represents a significant step forward in the development of 2d hybrids with mxenesgraphene for use in a wide range applications