Dragon09/odtu_chatbot huggingface dataset:
unravelling the chemicalphysical principles of life through minimal synthetic cellularity

a grand challenge in bottomup synthetic biology is to design and construct synthetic cells with lifelike properties from a minimal number of parts achieving this goal would be a major engineering feat and enable an understanding of how living systems work from the perspective of physical chemistry towards this we have exploited bottomup approaches and generated new insights into the impact of compartmentalization on the thermodynamics and kinetics of incorporated enzyme reactions our findings that dynamic coacervation can ignite dormant enzyme reactions provides the conceptual framework for our plan to build sustained outofequilibrium synthetic cellular systems in minsyn the aims are to 1 define how molecular reaction networks are tuned by compartmentalization 2 build minimal synthetic compartments with selfsustained outofequilibrium behaviour 3 utilize communication to coordinate reaction networks within populations of cells together these objectives test our overarching hypothesis that sustained outofequilibrium systems can be established by interconnecting three features molecular reaction networks compartmentalization and communication key to this endeavour is our unique combination of chemical biochemical and biophysical tools for quantitative characterization of synthetic cellular systems we are primed to address the major engineering challenge of building sustained outofequilibrium synthetic cellular systems and to tackle a central problem in biological sciences how do biological cells and tissues sustain life from collections of nonliving molecules our interdisciplinary approach will provide novel tools to the community and represents a unique multidisciplinary approach that will ultimately define the chemicophysico parameters of life this can lead to unprecedented opportunities to rationally engineer molecular systems which may supersede biological capabilities
toward an epidemiology of gynaecological violence

gynvepi will lay the foundations of an epidemiology of gynaecological violence gv gv refers to suboptimal experiences of care that can be perceived as abusive or dehumanising eg nonconsented medical acts discrimination understood as a structural and gendered form of violence it is a phenomenon that is yet to be fully defined and investigated in contrast to the neighbouring field of obstetric violence it has not been researched taking germany as an exemplary setting gynvepi aims at understanding the who the when the how and the health consequences of womens experiences of gv through an innovative intersectional genderexpansive and gendertransformative approach to social epidemiology it will provide a definition a framework and a quantitative validated instrument for the study of gv the mixedmethods reviews workshops interviews survey digital platform and interdisciplinary approach ensure the depth and strength of the findings gynvepi is important gynaecological care constitutes in many countries the first point of contact for womens health women are exposed throughout their life to the risk of gv and its toll is assumed to be large with consequences in the short to longterm on mental physical social and sexual health gynvepi is timely the momentum of obstetric violence research provides a good starting point to reflect on gv testimonies of gv outside the scientific arena urge researchers to grasp the topic digital tools and participation enable to collect effectively and quickly experiences of care users including among marginalised groups gynvepi will generate new knowledge on gv and its associations with health its gendertransformative component will initiate a transformation of harmful gender norms among the researchers and researched through reciprocal empowerment lastly the methodological approach will be applicable in further studies eg longitudinal studies and gv studies in europe
demystifying the impact of hivantiretroviral therapy on fertility and the epigenome of gametes and early embryos

hiv human immunodeficiency virus is a global epidemic that led to the death of over 401 million persons since its first report currently about 384 million people are living with hiv plhv globally 19 million in nigeria the introduction of combination antiretroviral therapy cart has improved the lifespan of plhv making it a chronic disease yet in 2021 about 650000 persons died from hivrelated illnesses efforts are being made to scale up treatment access to cart to reduce mortality however in recent years a not wellunderstood decline in fertility of plhv undergoing cart is a great concern for hiv treatment recent studies detected epigenetic abnormalities in the blood of plhv undergoing cart with global changes in dna methylation where it is unclear if cart or hiv itself could be the cause abnormal establishment of dna methylation in the gametes can lead to the manifestation of epimutations in the growing embryo with potentially bad consequences like embryo loss diseases and cancerogenesis this study aims to determine the impact of cart on fertility and on the dna methylome in gametes and derived embryos in the mouse as a model system it will involve the analysis of dna methylation changes in spermatozoa and oocytes derived embryos and offspring as well as the examination of embryonic genome activation in 2cell embryos and successful blastocyst development in early embryogenesis of mice under cart this study will contribute to health education about hiv and its treatment boosting the reproductive health of plhv and importantly improving their compliance with antiretroviral therapy thereby aiding the achievement of the unaids target of 2030 furthermore it will foster the mutual transfer of expertise in andrology and hivbiology to the host institution and will equip the applicant with novel skills in epigenetics and assisted reproduction relevant soft skills and new collaborations for his career development
proink4d engineering dynamic protein hydrogels for 4d bioprinting of advanced tissue constructs

the proink4d project embarks on a transformative journey to pioneer the development of nextgeneration proteinengineered biomaterials for 4d bioprinting of dynamic tissue constructs inspired by the intrinsic versatility of intrinsically disordered protein polymers idpps with lower and upper critical solution temperature lcst and ucst phase behaviors this project aims to create hybrid hydrogels by ingeniously combining protein polymers exhibiting opposite thermoresponse properties this innovative design strategy sets the stage for the creation of preprogrammed shapemorphing protein hydrogels paving the way for the development of responsive protein hydrogel actuators with enhanced efficiency with a comprehensive research plan spanning bioproduction material characterization bioink and bioresin optimization and advanced 4d bioprinting proink4d promises to make a significant impact on both scientific understanding and practical applications in the realms of tissue engineering and regenerative medicine moreover the project emphasizes rigorous project management research innovation and effective science communication to ensure the highest scientific quality and societal relevance of the generated knowledge and technology
disentangling the earlylife environmental determinants of pediatric liver injury an exposomewide approach

liverx addresses an important question how does the environment impact the developmental origins of liver health and diseasepoor liver health in childhood represents a major public health problem with longterm consequences elevated alanine aminotransferase a marker of liver injury and screening tool for pediatric nonalcoholic fatty liver disease nafld risk is increasingly reported at young ages currently being detected in almost one out of ten children living in europe and the western world although it is well known that an obesogenic diet and genetic predisposition play a role in the development of liver injury and disease the contribution of the complex environment in which we grow up and live remains unclear liverx addresses this knowledge gap the project will leverage the resources of the populationbased helix and generation r cohorts and follow an exposomewide approach to the study of environmental impact by using rich biomonitoring and geospatial exposure data collected at the vulnerable early stages of the lifecourse pregnancy and childhood advanced analysis techniques will be used to provide novel evidence on the individual and combined effects of a wide array of earlylife ubiquitous environmental factors including industrial chemical pollutants toxic metals air pollution and characteristics of the urban natural and built environment on liver injury markers in childhood and adolescence the project will also assess whether childrens diet quality and polygenic risk for nafld modify vulnerability to environmental impact and thus aid in identifying susceptible subpopulations and environmental priorities liverx fosters research cooperation across disciplines and knowledge transfer the project aligns with urgent challenges related to environmental pollution the urban environment and the health of future generations and thus its results are expected to be of high relevance for researchers policy makers and european citizens
smart solutions to empower small and mediumsized farms as guardians of the territory

ensuring a sustainable food system requires above all other factors reentrusting our primary production to the guardians of our territory the owners of small and mediumsized farms and farm structures in rural areas guardians focuses on understanding their challenges and empowering them with a set of digital innovative solutions that will help them to bridge the current technological gap between small and large farmsguardians will put in place a set of costeffective and humancentric digital solutions which aim to support and empower the penetration sustainability and resilience of more agroecological farming models like regenerative agriculture natural grazing alternative managed grazing strategies and the promotion of pollinators in different farm structures to do this a multiactor cocreation methodology a set of adoption pathways and 9 different technologies combining software and hardware ie drones sensors will be customized to cover the farmers needsthese digital solutions will be tested through a total of 27 small and mediumsized farms and farm structures with a wide range of production types arable crops woody crops grassland and livestock production in 6 pilots represented by farming cooperatives acting as one stop shops from 5 different countries with diverse environmental climatic and socioeconomic conditions as a result guardians will increase the uptake of innovative digital technologies by farmers guaranteed by the use of a multiactor approach integrating farmers and agricultural stakeholders at all stages of the project as well as scientists technicians and smescascade funding and connections to specific stakeholders agricultural cooperatives agrifood hubs dihs ogs will be used as multipliers to allow guardians to roll up its impact throughout europe it is expected to rollout and replicate guardians results up to 95 farms around europe during the project duration
preparatory phase for a european production capability to secure a supply of haleu fuel

highassay lowenriched uranium metal haleu is a critical resource required for the operation of research reactors and the production of pharmaceutical radioisotopes its availability is essential for advancing nuclear energy safety materials science basic scientific research and the performance of about 40 million nuclear medicine procedures worldwide each yearuntil recently eu has relied on russia and the usa for its supply of haleu russian supplies are expected to be unavailable for an extended period and the future availability of us supplies remains uncertain it is thus imperative for eu to establish its own haleu production capacitythe prephaleu initiative represents a preparatory phase aimed at producing essential components and evaluating the technical pathways for establishing this capacity in eu this project consortium brings together all key stakeholders including enrichment companies fuel manufacturers research organizations and medical radioisotope producers through this collaborative effort prephaleu intends togenerate substantial technical economic and regulatory information to support the decisionmaking processfoster alignment among the countries and parties involved in establishing a eu haleu capability as a shared assetwithin the framework of prephaleu the quantitative requirements for haleu metal will be updated and working groups will delve into enrichment metallization and transportation considerations the integration of these elementary bricks will be extensively discussed to create a coherent project dynamic and consistently consolidate results into an executive summary a key input for the decisionmaking phasethe prephaleu project initiated in response to the nrt0111 call for proposals is a cornerstone in the establishment of a eu production capacity for metal haleu it plays a pivotal role in securing activities in the fields of research healthcare and innovation throughout eu
unravelling spatiotemporal auxin intracellular redistribution for morphogenesis starmorph

starmorph is a groundbreaking endeavour with the ambitious goal of understanding plant organ morphogenesis which will pave the way to engineering plant growth this is of paramount importance for enhancing agricultural and forestry yields and hence contributing to global food security and environmental sustainability plant organ morphogenesis involves differential growth where various organ parts expand at different rates to create specific structures a significant challenge lies in understanding these differential growth programs while considering the mechanical constraints imposed by the tissues the plant hormone auxin plays a central role in differential growth it forms concentration gradients within tissues dictating the direction and rate of cell expansion mechanistically auxin can either repress or promote growth in a tissue and concentrationdependent manner but this biphasic behaviour remains largely unexplained the extracellular space cytosol and nucleus have distinct auxin perception mechanisms and hence starmorph pitches an auxin signature concept considering nuanced auxin levels in each compartment specifying an ensemble signal with quantitative and qualitative cell responses for morphogenesis currently auxin subcellular compartmentalisation is poorly understood and a key focus of the project is to provide a subcellular map of auxin dynamics within a growing organ to uncover how tissue mechanics interact with auxindependent growth processes which will be pivotal for understanding plant morphogenesis the starmorph project leverages a unique combination of plant cell and synthetic biology genetics biophysics and organic chemistry expertise this interdisciplinary collaboration aims to dissect plant morphogenesis from molecular to organ scales and has the potential to revolutionise our understanding of plant growth and development with applications in biotechnology and plant engineering
female bodies in sacred spaces reevaluating womens agency in the greek world

the project aims at reevaluating womens agency in the greek sacred world through the application of an innovative and interdisciplinary approach encompassing cognitive archaeology the role of women in ancient greece has been either overlooked or described as powerless controlled and excluded by scholars hogbin 1970 spain 1992 this research overturns this narrative by revealing evidence of powerful female agency in the ancient greek sacred space through a cognitive reading of the historical context and ancient mind which will also explain the reasons why women were highly involved and how their agency was fundamental for successful rituals
gravitational physics from the universe large scales evolution

how does gravity work on cosmological scales starting from july 2023 for the very first time in the history of space science the geometry of the universe large scales will be mapped by the euclid mission providing the scientific community with a wealth of cosmological data of unprecedented quantity and quality it will be a transformational moment that will open a new ground for testing gravity yet unless combined with a theoretical framework able to translate wonderful numbers into concepts this is bounded to be a missed opportunity this research program whose proponent is currently serving in the role of colead of euclid theory working group aims at constructing such a frameworkeuclid will compare the action of gravity on light with that on nonrelativistic particles in a regime vastly different from where general relativity has been confirmed any significant detection of a deviation will have profound consequences but how to confidently dissect and decipher these potential signals in the past years i have laid the ground work for cosmological tests of gravity culminating recently in a first reconstruction of gravity from available data these results are at their infancy and a leap beyond the current state of the art is urgently required to breach into the smaller nonlinear scales where statistical errors will be at their lowest the timing could not be better with data releases of euclid expected during the tenure of this proposalfrom an exhaustive exploration of the gravitational landscape under criteria of theoretical viability we will create predictions of the large scales phenomenology by broad classes of theories with an accuracy that meets euclid requirements these will be used to construct a comprehensive phenomenological framework extending into the nonlinear regime and inform bayesian nonparametric reconstructions of gravity from the latest data as a result we will provide a completely new view into gravity on large scales
framework for coordination of automated mobility in europe

fame will develop and validate common methodologies and tools to facilitate the sharing of best practices and lessons learned to support the collaboration within the community of ccam stakeholders across the complex crosssectorial value chain needed for the organisation and evaluation of largescale demonstration and future scaleup to the impacts of complete ccam solutions the mission of fame supports the commitment of the european commission and the ccam partnership to provide a longterm coordination framework for ri and largescale testing and evaluation activities in europe the establishment of a stakeholdervalidated european framework for testing on public roads including a ccam test data space tds a common evaluation methodology cem and of means for exchange of knowledge on ccam activities will improve cooperation consensus building and data sharing it will enable comparability and complementarity of the results of all testing and largescale demonstration activities in europe fame builds on a strong legacy of eufunded coordination and support actions which have developed harmonised methodologies and federated large networks of stakeholders to drive consensus building on challenges needs and requirements for ccam and field operational tests fame involves 23 partners and over 2000 subscribers of the current joint cad network which has collaborated with the european partnership on ccam partnership since its inception the development of the european framework for testing on public roads is at the core of fame the overall approach is built around three main building blocks and aims to engage with and enable in terms of capacity building stakeholders from the ccam area as well as capitalise knowledge about ccam through an integrated approach including stakeholder engagement wp3 research and innovation on the evaluation methodology and testing framework wp 4 and 5 and communication and knowledge sharing wps 1 and 2 to maximise impact
vascular control of nash progression

nonalcoholic steatohepatitis nash is a major risk factor for hepatocellular carcinoma hcc the 6th most common cause of cancerrelated death worldwide the transition among different nash stages to hcc stems from the complex interaction of multiple factors including gutliver axis modifications and a progressive disarchitecture of the liver parenchymathe central role of endothelial cells in regulating the metabolic crosstalk along the gutliver axis and in shaping the spatial organization of the liver parenchyma suggests a potential vascular control of nash progression however the possibility to precisely define the endothelial contribution is restrained by the limited ability to correlate gene expression profiling and functional readout such as protein phosphorylation with the complex morphological modifications occurring during nashto overcome these limitations we combined innovative spatial sorting strategies with transcriptomics and quantitative phosphoproteomics providing the first draft of the anatomical organization of proteins signaling in the liver vasculature moreover we unambiguously identified tyrosine phosphorylation the main target of the antiangiogenic therapy att as one of the most spatially regulated signaling eventbuilding on this expertise we will combine spatial sorting strategies in gut and liver with mouse models of nash progression and advanced imaging modalities to pursue the following aims1 provide a spatiotemporal characterization of the gut and liver vasculature undergoing nash development2 dissect the vascular determinants of nash progression3 identify the molecular mechanisms underlying the synergistic effect between aat and immune checkpoint inhibitiontogether our results will lay the foundation for understanding the molecular basis of a synergistic aat and immune therapy in hcc and help to identify novel prognostic markers as well as potential therapeutic targets
hydraulic compression for high capacity hydrogen refuelling station demonstration

h2refdemo aims to further develop and scale up by a factor of 5 the innovative compression concept developed in h2ref in order to address large vehicle refuelling applications requiring hydrogen to be dispensed at rates of hundreds of kgh such as bus fleet refuelling every evening at the bus depot truck refuelling and train refuelling thanks to demonstrating the process during one year for commercial 35 mpa refuelling of trucks the project will bring to trl7 the disruptive compression technology previously developed in the h2ref project and already validated for 70 mpa refuelling of light duty vehiclesalong with capacity scaleup h2ref demo will focus on process optimisation cost reduction and further durability testing full optimisation will be achieved by first developing a digital twin of the scaledup process use of accumulators with shells in hoop wrapped steel type ii a suitable technology for 35 mpa refuelling will allow to optimise costs a thorough accelerated testing approach involving at least 500 hours of continuous operation will allow to verify durability of the accumulators and the compression stages over the full range of operating conditions the demonstrated system is expected to provide a peak dispensing capacity of 150 kgh amounting to 1200 kgd with 8 hours of daily operation with a targeted cost of 1200 kgd the process is expected to reduce electricity consumption to 35 kwhkg of dispensed hydrogen from production on site at 2 mpa to vehicle tank at 42 mpa the knowledge gained will allow subsequent development to focus on commercial product development for short term commercial deployment a multidisciplinary team composed of 4 industrial companies and 3 rtos combining expertise in hydraulic power supply in bladder accumulator in process simulation modelling process digital twins in h2 refuelling and distribution stations is gathered in the consortium to reach the targeted kpis of h2refdemo