Dragon09/odtu_chatbot huggingface dataset:
tracing singlecell scale chemical signaling between interacting soil fungi

multiple species of fungi coexist in soils and play an important role in biogeochemical cycles to survive in a resource limited environment they have developed the means for interspecific communication and warfare via an arsenal of secreted secondary metabolites specific ecological role of those metabolites and the extent to which they affect biogeochemical cycling during fungal interactions remains unknown because they are secreted and act at a singlecell scale tracing them then and there can aid in identifying potential triggers for their production and clarifying their function currently used methods have either insufficient resolution or are destructive and are not suitable for such analyses here i will use my expertise in spectroscopy techniques to 1 establish experimental protocols for the singlecell scale fungal secondary metabolite identification and characterization using surfaceenhanced raman scattering sers microspectroscopy a method that employs optical properties of gold nanoparticles for molecule specific sensing and that has been shown in biomedical research to have an extraordinary potential for studying microbial metabolic processes i will combine it with microfluidics based soil chips that provide visual access to and mimic real ecosystems via control over biotic and abiotic environment of soil microbes ultimately my aim is to offer the community of soil fungal ecologists with a gamechanging new tool to study ecosystem functions of secondary metabolites in more realistic settings i will then use the approach to 2 determine how interspecific fungal interactions under varying nutrient conditions affect the composition of their secondary metabolome and its functions live and at singlecell scale additional transcriptome analysis will 3 reveal fungal genes involved in up or downregulation of the metabolite biosynthesis but also extracellular enzyme production for organic matter degradation and nutrient acquisition
intersectional inclusion in deliberation and participation with youth

sincrony aims to ensure the meaningful inclusion of youth who experience social disadvantage and marginalisation in deliberative and participatory processes dpp the project will target the factors and processes responsible for reproducing political inequalities in youth engagement in deliberative and participatory processes based on intergenerational and intragenerational power imbalances and youth intersectional positionalities the goal is to offer policymakers public servants researchers teachers youth workers facilitators of deliberative processes and youth csos concrete instruments guidelines and solutions to renovate deliberative and participatory practices implemented in public governance and schools the consortium of nine partners consisting of seven universities the alda european network of local democracies and an sme focusing on communication and dissemination will contribute multidisciplinary and multimethodological expertise in psychology education sociology political science gender studies social research intersectionality and media analysis a stakeholders collaborative network including expert advisors local municipality representatives schools civil society and youth organisations will also be engaged throughout the project together they will a enhance the inclusivity of deliberative practices in two local municipalities in italy and finland b design test and deliver an innovative coconstructed intersectional approach called the intersectional participatory action researchanddeliberation piloted in seven local municipalities and four schools in italy portugal the uk and denmark c design participatory formats d create tools e define ethical guidelines by improving the democratic quality inclusiveness effectiveness and legitimacy of deliberative and participatory processes the project will contribute to greater social justice for all youth
innovative eutectogels for emerging applications

the main goal of the ionbike 20 proposal is to fully exploit the science and technology of the disruptive eutectogel materials and its wide range of emerging applications in energy bioelectronics and environment ionbike 20 msca staff exchanges proposal is a natural followup of the ionbike rise project it will take advantage of the gained knowledge and momentum by the ionbike rise consortium in ionic liquid gels in bioelectronics with the addition of new partners as well as the extension of its technical scope to eutectogels and a wider range of applications ionbike 20 will provide support for a joint research and innovation project through the exchange of staff with 15 partners including sixe european universities one research institute two industries and six universities from noneu third countries ionbike 20 will encourage collaboration towards a common scientific goal between all partners through staff exchanges sharing research infrastructures and networking activities the research is highly interdisciplinary involving experts in different areas such as chemistry polymers electrochemistry organic electronics bioelectronic devices energy chemical engineering environmental membranes and medical sciences ionbike 20 is very ambitious and it goes all the way from chemistry of the emerging eutectogels towards applications including the participation of two industrial partners
enhanced global quantification and understanding of nitrous oxide emissions from agricultural soils

agricultural soils are the primary humaninduced source of atmospheric nitrous oxide n2o emissions a potent greenhouse gas and the most significant damaging substance of the ozone layer climate change and the projected increases in food demand agricultural land area and fertilizer use are expected to increase n2o emissions over the coming decades however the actual magnitude and the keyinfluencing factors remain highly uncertain colossal will develop an innovative modelsdriven framework to improve global quantification and understanding of n2o emissions from agricultural soils first i will produce comprehensive databases of n2o emissions and crop productivity at site and regionalscale establishing the foundation for subsequent steps second i will use an advanced terrestrial biosphere model for a mechanisticbased quantification of global n2o emissions from agricultural soils covering historical and current time periods third i will implement interpretable machinelearning models to unravel the key drivers and estimate n2o emissions globally including plantsoilatmosphere interactions and management practices finally i will unfold future n2o emission scenarios by incorporating climate change projections and mitigation practices into the terrestrial biosphere model informed by interpretable machinelearning models colossal stemming from 3 leading multidisciplinary research groups will take place during the 2year outgoing phase at the massachusetts institute of technology including an 8month secondment at boston college usa and 1year return phase at aarhus university denmark overall colossal will advance our understanding of n2o emissions from global agricultural soils contributing decisively to the design of more effective strategies for mitigating worldwide greenhouse gas emissions in the future
strategies to modulate the bioavailability of cannabinoids in edible products in vitro tests cytotoxicity and preclinical assessment to generate reliable data for regulatory agencies

cannabis edibles ce are considered a big opportunity to take place in the recent and fastgrowing cannabis market however there are big challenges on the development of these products such as the lack of understanding of the metabolism of cannabinoids after oral ingestion the low bioavailability of cannabinoids including cannabidiol cbd and high intra and intersubjects pharmacokinetic variability recent approaches to enhance the oral cbd bioavailability include its incorporation into lipidbased delivery systems and the addition of compounds called bioenhancers lipids can enhance the oral cbd bioavailability via an increase in the transport to the systemic circulation via intestinal lymph whereas bioenhancers may inhibit metabolizing enzymes reducing the extent of its liver firstpass effect piperine pip is known as a powerful bioenhancer by inhibiting drugmetabolizing enzymes of cytochrome p450 cyp450 that are involved in the cbd metabolism the aims of this study are to provide reliable data about the cbd metabolism after oral ingestion to help regulatory agencies to regulate the market of ce and standardize the consumption indications for these products to increase the cbd bioavailability by using longchain fatty acids to promote the enhancement of lymphatic absorption combined with pip addition to inhibit the hepatic metabolism of cbd and develop an advanced delivery carrier to enable its vehiculation into aqueousbased ce we hypothesize that the combination of cbd and pip can substantially improve the cbd bioavailability thus decreasing the intra and intersubject variability systematic in vitro tests will be performed to evaluate the inhibitory effect of pip on cyp450 activity to determine the bioaccessibility stability and bioavailability of cbd and pip after oral ingestion and to assess their cytotoxicity finally in vivo studies will be performed to evaluate the cbd pharmacokinetic and bioavailability
immune cell death zonation regulated by protein hubs in plants

agriculture which underpins the livelihoods of over 25 billion people is nowadays confronted with an array of new threats including plant pests this project tackles the urgent need to develop new technologybased sustainable strategies to combat the constant agricultural losses and economic drain derived from plant pests which have been worsened because of climate change the understanding of the molecular and cellular mechanisms underlaying plant pathogen interaction and defence responses is not only a fundamental scientific challenge but it is also relevant to practical applications despite decades of research on hostpathogen interactions knowledge on fundamental aspects of the plant immune system is still missing this proposal provides an excellent opportunity to help filling this gap of knowledge as it targets the question of how cells translate molecular pathogen recognition into immune cell death and how dying cells and their neighbours communicate to tightly control this process and ensure the proper balance in between defence responses and plant growth and fitness to address this question i have designed a multidisciplinary and international project using arabidopsis thaliana plant as a model system to study the molecular determinants of plant immune cell death zonation and the involvement of plant metacaspases distant relatives of animal caspases in cell death a plethora of techniques including molecular and biochemistry techniques plant phenotyping and engineering facs and cell biology microscopy techniques will be used for the proper execution of this project in addition to generate highimpact knowledge in plant immunity that will ultimately impact on society this proposal will provide me with the expertise resources training and network to become extremely competitive in the field of molecular plant pathology helping me to progress in my scientific career and to achieve my scientific goals
electrified single stage ammonia cracking to compressed hydrogen

single will enable ammonia as an energy carrier in the hydrogen value chain through demonstration of a proton ceramic electrochemical reactor pcer that integrates the ammonia dehydrogenation adh reaction hydrogen separation heat management and compression in a single stage the realization of 4 process steps in a single reactor allows the technology to achieve unprecedented energy efficiencies with a project target to demonstrate 90 hhv at system level the pceradh technology enables to directly deliver purified pressurized h2 20 bar single will demonstrate the technology at a 10 kg h2 day scale that will provide a pathway for future scaleup systems ranging from small fuelling stations to large centralized at harbour deployments a key technology component is the electrochemical cell that will be engineered to act as a durable pgmfree adh catalyst at 500 c and a voltagedriven membrane separator the achievements in single will be an important proof of technological feasibility advancing the technology from trl3 to trl5 to strengthen the implementation of nh3 as a h2 carrier single will actively disseminate and communicate the results to influence stakeholders in the value chain including standardization entities within the hydrogen sector the consortium counts on partners from the industry institute and academia sector with high worldwide excellence in the respective fields of catalysis electrochemical membrane reactors lifecycle assessment process engineering control systems and hydrogen fuelling stations
reversible gray debugging of concurrent systems

the project tackles gray debugging of concurrent systems gray debugging means debugging systems where only part of the source code is accessible eg the system invokes external services such as google maps debugging concurrent systems from multiprocessors to cloud is notoriously hard reversible causalconsistent debugging and replay rccdr is an innovative approach to this problem allowing one to log a faulty execution in production environment and replay it in the debugger there it can be explored backwards and forwards following causality links from the visible misbehavior to the bug causing it this approach has been implemented in cauder a debugger for the erlang languagecurrently rccdr requires access to the whole source code of the target program to enable gray rccdr we need to answer the following questionshow can we define rccdr and more generally reversibility in concurrent systems in a compositional way to be able to apply it to systems which are only partially accessible are we able to correctly explore backwards and forwards the accessible part if not which approximation in the exploration is caused by the missing information about the inaccessible part till now rccdr has only been explored in the concrete setting of erlang and gray rccdr has never been studied we plan to study gray rccdr at two levels1 the abstract level of event structures abstraction ensures simpler proofs and generality of the results making them applicable to many languages2 the concrete level of erlang semantics ensuring applicability in a setting of interest and allowing to implement it by extending cauder and integrating it in the erlang solutions environment as an outcome we will provide the theory to apply the powerful rccdr approach to concurrent systems which are only partially accessible as well as exploit it in practice in the setting of erlang this will also enable interaction with it smes and software developers
bridging accessibility participation and inclusion with automatic live subtitles

background accessibility is usually understood as means of making cities more accessible for people with mobility disabilities but accessibility encompasses also access to culture and information public institutions communicate with their residents and visitors through various channels yet what about those who cannot access this information such as people with sensory disabilities or as is common in the eu people with different linguistic backgrounds a way to provide universal access to audiovisual products is using automatic subtitling an aibased technology that automatically generates subtitles for prerecorded and live audiovisual products aim i will address the role of automatic subtitling in enhancing the accessibility of communication in public institutions and its potential impact on the wellbeing and civic engagement of citizensurgency advances in artificial intelligence have improved drastically the quality of live automatic subtitling but we do not know how this technology impacts its users in several communicative situations since there are no reception studies on this matterapproach this is an empirical and interdisciplinary study mixing ethnographic and experimental approaches using both qualitative and quantitative data collection tools this is the first study investigating the interaction between live automatic subtitling and its end usersinnovation and impact 1 research into a new unclaimed research avenue 2 interdisciplinary approach with data collection tools never used before on media accessibility 3 determine the impact of technology on reception of media accessibility 4 results will directly improve several stakeholders lives 5 results will be directly applicable to professional practice promoting knowledgebased economy
validation of lunar water extraction and purification technologies for insitu propellant and consumables production

sustainable space exploration requires the development of insitu resource utilization isru technologies which encompass all processes that utilize local resources to generate useful products for robotic and human exploration among the available resources water is the most versatile and most needed in space exploration water can be easily stored and directly used as consumable for astronauts or electrolyzed to hydrogen and oxygen a very effective rocket propellant combination luwex aims to develop integrate and validate lunar water extraction and purification technologies for insitu propellant and consumables production for future space exploration missions the consortium will develop key technologies for an insitu raw water process chain which include an innovative water extraction prototype water purification technologies and water quality monitoring for application in a future europeanled space exploration mission an integrated test setup will be built to validate the operational capabilities of the technologies and also of the whole process chain this setup will deliver a relatively realistic environment analogue to the lunar surface and will use a lunar dustice simulant to provide proper validation conditions to raise the trl of all subsystems and the whole process chain to level 4 the interdisciplinary nature of luwex combines research and innovation in space engineering space science and exploration geophysics and terrestrial water systems large space industry thales alenia space research organizations dlr sme liquifer systems group scanway and academia technische universitt braunschweig wroclaw university of science and technology from four european countries germany italy austria poland join forces in a unique consortium to enable breakthroughs in isru technologies luwex will help to strengthen the excellence in european space science and exploration and also foster the competitiveness of the european space sector
turbulence pebbles and planetesimals the origin of minor bodies in the solar system

the minor bodies of the solar system asteroids trojans comets and kuiper belt objects are left over planetary building bricks called planetesimals once abundant in the solar nebula via collisions and accretion of large grains aka pebbles they grew into planets the efficiency of planetesimals formation via a gravitational collapse of pebble clouds and the characteristics of the forming planetesimals are determined by the size distribution and local concentration of the largest grains both being regulated by gas turbulence but turbulence itself is dependent on the abundance of small grains by regulating the ionisation level and the radiative cooling processin this project we will develop radically new types of numerical experiments of three stages of planetesimal formation with the goal of a selfconsistent turbulence and pebble size distribution1 development of tools to measure the transport diffusion and collisions of dust grains for arbitrary mhd or radiation hydro disk simulations to derive via an coagulation code and machine learning technique a consistent particle size distribution for consistent opacities and ionisation rates to feed back into the turbulence simulation 2 implementation of a treesolver for the gravitational attraction among pebbles in selfconsistent turbulence simulations to identify the properties of pebble clouds that can undergo gravitational collapse 3 integration of an implicit solver for our lagrangian particle scheme to model the collapse of pebble clouds while not only deriving a mass function and multiplicity but with a model for elasticity and porosity analyse the spin shape and compression for the forming planetesimals comets and asteroidsin close collaboration with our scientific community we will calibrate our turbulence models and the planetesimal formation process on the observation of disks around young stars as well as observational and laboratory data on minor bodies in the solar system
realtime highcontent superresolution imaging of es cell states

the development of superresolution sr microscopy in recent years has revolutionized cell biology breaking the diffraction limit of light microscopy by order of magnitude however sr is currently incompatible with highcontent imaging rtsuperes will provide a groundbreaking and affordable technology with automated sr capabilities beyond the stateoftheart to this end we will generate a library of endogenouslylabelled snaptag fusion proteins in mouse embryonic stem cells escs and deploy a realtime decisionmaking module which will continuously monitor our snaptagged cells using fast fluorescence imaging and once a change is detected will fix the desired cells and switch to sr mode by bringing together seven worldleading experts from four different countries combining basic and applied research and industry we propose several firsts a the first endogenouslylabelled clone library of snaptag fusion proteins b utilize machine learning ml for realtime automated decision making autonomously switching from fast conventional to sr imaging c combine high content with sr imaging d integrate novel cuttingedge technologies namely sr radial fluctuations srrf nanojfluidics single molecule localization microscopy smlm and structured illumination microscopy sim e collect large scale imaging datasets of cell states in escs and f provide cellcycle stagedependent nanoscale localization of selected nuclear and chromatin proteins eg h33 during early esc differentiation rtsuperes will provide the scientific community with the firstofitskind commercial realtime srhighcontent imaging system and the first library of endogenously snaptagged esc clones which are ideal among many other things for sr imaging