Dragon09/odtu_chatbot huggingface dataset:
ultrathin twodimensional polymer heterostructure membranes enabling unidirectional ion transport

current separation technology is crucial for many aspects of human life and accounts for 15 of the worlds energy consumption while the particle flow through separation columns is directional at the atomistic scale undirected brownian motion dominates in stateoftheart membranes 2d membranes have the potential to overcome this intrinsic deficiency and shift the paradigm of particle transport from disordered brownian motion to unidirectional flowwe will develop unprecedented 2d polymer heterostructure membranes 2dhms combined with functionalized graphene they offer ultimate thinness leading to shortest diffusion lengths precision pore geometrysize resulting in high sizeselectivity even for hydrogen isotopes and high functionality fostering chemicalcharge selectivity and ionic gating making them ideal membrane materials to realize selective and unidirectional ion transport we will combine our complementary expertise in theory and prediction chemical design and onwaterliquid surface synthesis as well as insitu ion transport investigations to develop robust 2dhmswe will synthesize 2dhms in the form of horizontal and vertical heterostructures for which reliable structureproperty correlations will be established we will take advantage of lattice vibrations nuclear quantum and electrochemical effects and consequently reformulate classical diffusion theory to consider these game changers as a result we will achieve innovative 2dhms for selective proton and ion transport with high permeance laying the foundations for the nextgeneration membrane technologies2dpolymembrane will unlock the unique opportunities of 2dhms for innovative energy device integrations protonaqueous metal batteries fuel cells and reverse osmotic power generators where the merits of ultrathin precision 2dhms will result in the highest selectivity and highest particle flow and thus a fundamental device performance beyond the stateofart
international conference on research infrastructures icri 2022 in brno

international conference on research infrastructures icri is a major event bringing together policy experts facility managers leading researchers and a variety of other stakeholders to discuss challenges and emerging trends for research infrastructures ris in the global context the aim of the project is to facilitate the organization of icri 2022 conference in brno czech republic in october 2022the conference will be coorganized by masaryk university mu the second largest czech university central european institute of technology ceitec interdisciplinary centre of excellence cohosted by mu and 5 more brno universities and research institutes the czech ministry of education youth and sport meys and the european commissionthe objectives of the conference are1to explore the role of research infrastructures in tackling current and future societal challenges eg that of postcovid recovery and international collaboration2to explore organizational models and operational systems of research infrastructures eg concerning data management integration of smaller sized facilities promotion or impact assessment3to allow community building in the area of research infrastructure policies and management4to promote the stories of host country region city and institution as dynamically developing areas fuelled by research infrastructures5to promote science and technology as the driver of societal and economic progress among general public highlighting the role of research infrastructuresimproved policy and management in the area of research infrastructures may have widespread impact across regional economies and may significantly contribute to tackling global challenges icri 2022 will facilitate sharing of knowledge and spreading of best practice on how to efficiently organize ris
deciphering and exploiting the chemical features of silent lipopolysaccharides a gift from gut microbiota

gut microbiota is a key actor for human health driving many physiological and pathological processes including immune system development and modulation how this massive population of microorganisms most of which are bacteria establishes commensal mutualistic or pathogenic interactions with the human host despite the vigilance of the immune system is still obscure and requires an indepth study the story gets more intricate considering that gut is home for a myriad of gramnegative bacteria whose outer membrane main constituent is the lipopolysaccharide lps due to its chemical structure lps is considered a potent elicitor of immune inflammatory reactions in mammals being usually associated to perilous bacteria and detrimental outcomes for human health nevertheless lps also decorates the membrane of harmless and beneficial gramnegatives of gut microbiota how lps is tolerated and remains apparently silent in the gut is a major unsolved question representing a frontier in our understanding of innate immunity debugginglps project will contribute to answer this question starting from the assumption that the chemistry of lps is the real message taken from human host of the bacterial interaction either beneficial or harmful strategically based on my expertise in organic chemistry and integrating synthetic chemistry and cellular immunology studies debugginglps will decrypt the chemical language spoken by lps in the gut this project will deliver a clear picture of the chemistry at the basis of the difference between good and bad lps providing tools for the exploitation of the acquired knowledge to create novel therapeutics for resolvingmitigating immune disorders debugginglps has been conceived to go beyond the stateoftheart breaking the dogma of lps as an enemy leaving space for a new vision of this glycomolecule ie no longer as a toxic bacterial product rather as an immune signal vital for the proper functioning of our body
network for novel remote sensing technologies in forest disturbance ecology

the proposed project enhances networking activities between research institution in widening country institute of forest ecology slovak academy of sciences ife sas and topclass counterparts at the eu level finnish geospatial research institute the university of eastern finland and swedish university of agricultural sciences the project builds on networking for excellence through knowledge transfer and exchange of best practices between involved institutions the major result will be raising reputation research profile and attractiveness of ife sas project implementation will enhance ife sas staff management capacities administrative skills and scientific capabilities in the use of novel remote sensing technologies rst in forest disturbance ecology fde the project proposes establishment of initial network and development of a new joint research project in novel rst applications in fde rigorous analyses of severe insectinduced disturbances using novel rst will be carried out in test areas representing different forest and climate types mountain forests in slovakia and boreal forests in finland and sweden we will integrate in situ uav and drone acquired remotely sensed data existing multitemporal geospatial information and field data particularly data on bark beetle population density visible infestation symptoms linked to outbreak phases and tree physiology parameters measured using electronic dendrometers or sapflow meters the combined dataset will be used to develop new tools for landscalelevel early bark beetle attack identification and for designing bark beetle infestation risk assessment model we will draw on the latest advances in drone technologies and image analytical tools including deep convolutional neural networks based machine learning techniques and artificial intelligence algorithms we expect to obtain important scientific results and contribute new knowledge to this scientific field
composting as a posttreatment method to mitigate the transfer of antimicrobial resistance and sporeforming firmicute into the food chain resispore

the use of animal waste as agricultural fertilizers is a potential source of pathogens antibiotic resistance genes args and antibiotics residues abs and must be managed accordingly pretreatments of organic waste prior to land application offer the potential to abate enteric bacteria and therefore reduce the risk of contamination of crops or adjacent water resources with resistant pathogens carried in these materials however the reduction and fate of such fecal indicator bacteria may not properly predict the responses of of all types of pathogens especially those that produce spores the inactivation of sporeforming pathogens such as clostridium and bacillus spp from animal waste could be challenging given the recalcitrance of the spores these bacteria produce resispore is an interdisciplinary and innovative project in the field of environment agriculture that aims to identify intervention management strategies to stop the initial movement of antimicrobial resistance amr and sporeforming pathogens from animal waste into the environment and food chain resispore aims are aligned with current european strategies to produce safe healthier and more sustainable food such as the farm to fork strategy or the efsa recommendations for protecting food safety this project will be delivered by the fellow dr jessica subirats with a strong expertise in environmental microbiologist under the supervision of drvictor matamoros who has a strong trackrecord in environmental biochemistry and agricultural science the fellowship will provide new expertise and training on analytical chemistry largescale biogas operations agricultural science and professional development for academics the fellow will bring her knowledge of amr and microbiology using both culturedependent and independent methods the proposed work will expand the fellows research competencies and professional networks enhancing the development of her career as an independent researcher
geometry and analysis for gxstructures and their deformation spaces

the study of geometric structures on manifolds finds its inspiration in kleins erlangen program from 1872 and has seen spectacular developments and applications in geometric topology since the work of thurston at the end of the 20th century geometric structures lie at the crossroads of several disciplines such as differential and algebraic geometry lowdimensional topology representation theory number theory real and complex analysis which makes the subject extremely rich and fascinating in the context of geometric structures of pseudoriemannian type the study of submanifolds with special curvature conditions has been very effective and led to some fundamental questions such as the open conjectures of andrews and thurston from the 2000s and the recently settled labouries conjecture this project aims to obtain important results in this direction towards four interconnected goals 1 the study of quasifuchsian hyperbolic manifolds in particular leading to the proof of a strong statement that would imply the solution of the conjectures of andrews and thurston 2 the achievement of curvature estimates of l2type on surfaces in antide sitter space 3 the construction of metrics of parahyperkhler type on deformation spaces of gxstructures and the investigation of their properties 4 the study of existence and uniqueness of special submanifolds of dimension greater than 2 in pseudoriemannian symmetric spaces the project adopts an innovative approach integrating geometric and analytic techniques and the results will have remarkable applications for teichmüller theory and anosov representations in the long term the proposed methodology and the expected results will lead to further developments in various related directions for instance the study of pseudoriemannian manifolds of variable negative curvature of higher dimensional pseudohyperbolic manifolds and the deformation spaces of other types of gxstructures
feasibility of a secretome factory to treat paediatric interstitial lung disease

despite their astonishing clinical success on adults there are still no mesenchymal stem cell mscbased products approved for paediatric use preclinical studies support a potential beneficial role of msc therapy to prevent progression in diseases called childhood interstitial lung disease child however mscs still suffer from the cost of culturing and storage facilities and a lack of standardization of cell populations their quality and quantity donorrelated factors protocols for isolation in vitro expansion cell delivery and dosing our goal is to standardize and automatize the production of msc secretome and validate it for its translation to paediatric use to treat child we will explore the feasibility of a secretome factory based on 1 isolation of mscs from donor bone marrow 2 expansion of mscs in a micro scaffold called nichoid and 3 longterm culture of msc for secretome production in an optically monitored bioreactor called moabnichoidto achieve these goals in this poc we will validate the main key performance indicators of the production process and prepare a gotomarket strategy for the secretome biodrug our outcomes will be 1 a moabnichoid production line with total costs compatible with its industrial production and marketing by a pharmaceutical company 2 standard operating procedures sop for the production process in full compliance with the gmps in the sector of cell and tissue therapy products ptc 3 secretome quality parameters obtained through a comprehensive characterization and 4 demonstration in vitro of the safety and therapeutic efficacy in the pathologies studied the outcome of this project will provide a national and international reference point for the industrial production of msc secretome to treat child and then extending this approach to other pathologies also affecting adult patients including chronic intestinal disease and osteoarticular diseases
diffractive optical element fabrication based on 3d printing

diffractive optical elements does are used to shape the wavefront of incident light in complex patterns and are ubiquitous across optical applications ranging from laser processing through lithography to communication and imaging despite their popularity fabricating does is nontrivial mostly due to their nanoscaleprecision requirements necessitating highly precise expensive and cumbersome fabrication methods typically using photolithography which are also limited in the design flexibility they allow during the pis ercstg we developed a method to enable simple fast and highquality fabrication of does by combining 3d printing with nearindex matching by liquidimmersion this results in does comparable in performance to the stateofthe art yet manufactured at a fraction of the cost and time still at this point these does need to be immersed in a small liquidcontaining chamber to operate which prevents mass adoption of the technique by the market hence the main goals of this poc we will adapt our 3dprinting based nearindex matching technique to yield fully solid does fabricate and demonstrate several proofofconcept industryrelevant elements and explore various strategies to bring our technique to the market our technology has the potential to transform the world of doe fabrication by drastically simplifying the fabrication process shifting the relevant scales from weeks to hours from thousands of dollars to a few dollars per element and finally alleviating the need for a cleanroom environment which will significantly democratize the process of doe fabrication making it available in lowresource settings
creation of a glp bank of immuneprivileged immortal mesoangioblasts to treat monogenic recessive diseases of muscle and connective tissue

stem cells led to effective cures for diseases of blood and epithelia however in the case of diseases affecting muscles connective tissue or brain significant challenges persist for expansion of a sufficient number of corrected cells delivery and engraftment even when these problems will be solved the prospective perpatient cost of the treatment up to 2m will make such therapies unsustainable for nhs we developed protocols of cell therapy using mesoangioblasts mabs vessel associated progenitors that despite promising results in animal models showed modest efficacy in patients to address this we transplanted autologous dystrophic mabs expressing a small nuclear rna engineered to skip dystrophin exon 51 that enters and corrects also neighboring nuclei of the multinucleated muscle fiber thus bringing dystrophic production to therapeutic levels a phase iiia trial is currently running even in case of success the cost would remain prohibitive through the ongoing erc adg 884952unimab we are completing the development of immortal immuneprivileged mabs from dystrophic patients thanks to genome editing of hla and the expression of tolerogenic proteins in this poc project we will produce universal mabs from healthy donors and will study the feasibility of producing and storing a large amount of universal mabs through a bank of universal donor mabs from healthy patients ready to be corrected for the specific genetic mutation and injected into the patient following the motto one serve many available cells a gmpgrade lentivector expressing the wt cdna of the mutated gene and a mouse model for preclinical tests would be sufficient to start a trial even for extremely rare diseases that currently lack even the hope of a therapy this work will enable the business exploitation of the mabank technology by creating a company with a gmp biobank that may operate internally and make cells available to clinicians and biotech companies
earth observation multimission federation layer

the dominoe project proposed by a consortium of european organisations including scientific institutes and smes and led by airbus defence and space aims at solving the key challenge of availability and reactivity of earth observations from space by enabling multimission accessibility on a scalable and automated way the implementation of a multimissionmultisensor federation layer allows the enduser to address a variety of acquisition assets using scheduling and optimization algorithms the orchestration between the users patrimonial missions and the third party missions is based on reactivity persistence precision and costs criteria while user experience is improved thanks to cognitive assistantsthe challenge is to overcome the current technological architectural and economical roadblocks of existing mission ground segments monomission architectures unharmonized interfaces between different ground segments inexistent or crude multimission collaborative coverage and dispatch servicesdominoe consists of designing analysing and modelling the multimission federation layer based on users requirements and is supported by demonstrations of added value services a market analysis is performed to assess the commercial perspectives of multimission federation approach in terms of client acceptance in sharing assets industrys make or buy strategy and sme capability to build catalogues of multimission services this innovative federation layer supports the change of space industry paradigm from instrumental push to endclient vertical needs pull and allows the eu space industry to embrace the emerging data driven space market in such dominoe contributes to european nondependence for the development of earthobservation technologies and foster european competitiveness by supporting smes in developing multimission services agnostic to the endtoend or ground segment systems integrators
efficient safe and costefficient rna delivery vehicles for hardtotransfect preclinical and therapeutic cells

human induced pluripotent stem cells hipscs have revolutionized the study of cell typespecific processes and the generation of organoids tissues and therapeutic cells for biomedical purposes however the genetic modification of these cells along with other difficulttotransfect cells poses a major challenge for performing highthroughput gene reporter and genetic perturbation assays and prevents us from fully exploiting the potential of hipscsexisting gene delivery techniques such as lentiviruses or lipid nanoparticles suffer from limitations in precision biosafety efficacy and high production costs to overcome these limitations our team has developed a novel approach called internalizers which offers a genetically controlled cellular production process for nonviral rna delivery systemsinternalizers can enable transient gene expression and modular gene editing in virtually any cell type including differentiated hipsc and t cells the method demonstrates high efficacy and cost efficiency while maintaining biosafety levels comparable to s1 standards we aim to position internalizers as a promising alternative to lentiviruses and lipid nanoparticles opening up new possibilities in gene delivery applications for preclinical research and therapeutic cell systems
breaching the protective cancer stroma with radiotherapyresponsive liposomes

liposomes have been the most successful type of nanomedicine for cancer patients playing a leading role in improving the tolerability of chemotherapeutics however to advance the success of liposomal drug delivery and cancer treatment new approaches to physically trigger drug release in cancer tissues and increase the permeability of the protective cancer stroma are needed this project will investigate whether radiocatalytic nanomaterials can be integrated in liposomes for spatiotemporalcontrolled drug release the alleviation of cancer desmoplasia and improved efficacy of coencapsulated drugs liposomes will be composed of oxidationsusceptible phospholipids that encapsulate hydrophilic drugs in their aqueous core radiationabsorbing nanomaterials will be included in the liposomes to produce high levels of reactive oxygen species when activated by xrays this will oxidize the liposomes and facilitate the release of the encapsulated drugs simultaneously the cytotoxicity of the oxidants is expected to reduce the high cell densities in the tumor periphery enabling the therapeutics to deeply permeate the cancer tissues following their release this customizable strategy will be developed in the context of pancreatic cancer a chemoresistant form of cancer with poor survival rates this project develops a multidisciplinary approach to investigate the physicochemical mechanisms of radiotherapyinduced drug release adaptive optics microscopy to study tissue permeability in 3d cultures of desmoplastic pancreatic cancer and multimodal in vivo imaging to uncover the therapeutic potential and mechanisms radiotherapycontrolled drug release from liposomes is a groundbreaking novel concept and its capacity to increase cancer tissue permeability has never before been demonstrated when successful new avenues for controlled liposomal drug delivery will emerge that can significantly advance the standardofcare for many cancer patients