Dragon09/odtu_chatbot huggingface dataset:
building emotional circuits interfacing intrinsic programs with earlylife experiences

emotion regulation and responses to fear and stress are vital for survival and interactions with the environment within the brain the amygdala governs emotional states through specialised nuclei and connectivity controlling anxiety fear reactions and emotional learningin mammals amygdala circuits mature postnatally and are influenced by motheroffspring bonding which impairment causes earlylife stress and leads to lasting consequences on anxiety however the molecular and cellular mechanisms underlying amygdala neuron differentiation and wiring their reliance on intrinsic programs potential sex differences and impact of earlylife experiences remain poorly understoodhere i hypothesise that the differentiation of amygdala neurons relies on transcriptional programs which vary between sexes and are modulated by earlylife stress building on advanced technologies and expertise for singlecell gene expression spatial position and connectivity analyses that i developed in my prior work i propose to test this in the amygdalae of female and male mice by1 identifying transcriptional programs guiding neuron differentiation using singlenucleus and spatial transcriptomics2 characterizing projection development and intrinsic programs using barcoded axon tracings combined with transcriptomics3 determining the role of intrinsic determinants and the impact of earlylife stress on projection development and function using crisprcas9mediated gene manipulation maternal separation 3d axon tracings and anxietyemotional memory teststhese experiments will reveal transcriptional programs governing amygdala circuit development and sex dimorphisms they will identify amygdala neuron types sensitive to environment potentially more prone to be affected in diseases this research will contribute to understanding the genetic and environmental aspects of emotional dysregulation a common feature in neuropsychiatric disorders including anxiety disorders
thermostable vaccines to enable global immunization

with globevac we present a novel solution to make vaccines thermostable enabling global immunization by avoiding the need for cold chain logistics vaccines are one of the most successful public health interventions to reduce the morbidity and mortality of infectious diseases to ensure the safety and effectiveness of a vaccine the production and distribution of almost all vaccines require what is called a cold chain where the vaccine is continuously kept below certain threshold temperatures sometimes as low as 80c the need for such a cold chain inhibits costefficient and rapid distribution of vaccines and significantly reduces immunization in low resource settings our globevac approach consists of storing the vaccines in a novel formulation making them thermostable for weeks when kept at temperatures as high as 40c preliminary in vitro data has shown that our globevac solution can be applied for several classes of vaccines globevac formulated adenovirusbased vaccines and vaccines based on enveloped viruses that were stored at 40c were as active and functional as the fresh ones kept at 80c in this erc poc we will technically validate our novel formulation for different classes of vaccines next to that we will explore the commercial potential of globevac by developing an intellectual property strategy and performing an extensive market analysis that will finally lead to the development of a tailored business strategy
integrating a comprehensive european approach for climate change mitigation and adaptation in montenegro viticulture

the principal objective po of montevitis is thus to strengthen homogenize and augment udg and its involved faculties and labs research and collaboration capacities and performance both in montenegro and across the european prospect in specific topics of climate change mitigation and adaptation in viticulture three defined operational topics constitute the pertinent needs of an integrated and operational european approach in tackling climate change in viticulture now to be applied in montenegro via udg firstly the project will set up integrated and validate the following topics 1 use and adaptation of existing analytical and modelling tools for assessing the effects of climate change at regional levels 2 understanding these effects on the course of physiological processes in the vine yield ripening and quality of grapes and wines of different varieties montevitis will then combine a somewhat orthogonal topic coming from the ict sphere of knowledge for efficiently applying the emerging technologies for enhancing the mitigation and adaptation measures in vineyards 3 definition of precision viticulture technologies and usage scenarios in vineyard management the project is built upon the strengths of all of its consortium members and conducts the twining processes in combining the research methodologies with gradually expanding knowhow and findings for the benefits of udg and all participants montevitis eu partners are in countries with worldwide recognised viticulture demarcated areas and have years of rd and innovation experience in viticulture and climate studies as well as being able to share valuable knowledge and experience all partners will benefit from research networking capacity building and knowledge transfer processes within this action and ongoing research projects and the general strengthening of many sustainability components udg also invited several partners from montenegro for extending the impact of the project
compliant and fully automated circular solutions for multiple battery and battery embedded device enhanced by digital solutions

the increasing need to find alternative energy sources combined with the general urgency to decarbonise the way we produce store and consume in order to meet the eus climate and sustainability targets is stimulating a massive and rapid use of critical raw materials which are fundamental to the production of electrical and electronic equipment and batteries pillars of the green and digital transition that the eu is called upon to achieve the challenges are increasingly important given the rapidly changing geopolitical environment automat is responding to these crosscutting and urgent challenges through the timely introduction of disruptive holistic circular and sustainable targetdriven concepts and solutions driven by the implementation of the following key aspects i high valueadded recycling technologies mainly focused on preparing batteries and eee components battery packs modules cells parts and materials for reuse through the most advanced collaborativeadaptive and flexible disassembly sorting and material recovery technologies and integrated solutions in order to drastically reduce the amount of nonreusable parts and materials and to return to the market with new products including chemicals able to meet the needs of the market ii transversal digitisation of solutions towards a multilevel and continuous digital data exchange and elaboration able to support new strategies of advanced and compliant separate collection to increase the quality and availability of target streams support in real time the efficiency of recyclingreuse processes through the homogenisation of external inputs provide always personalised and predictive information on the most appropriate operations to be applied aiming at a massive reduction of risks and waste during handling collection and recycling operations provide digital passports to second life applications to strengthen the efficiency of the outputs coming from the automat global value chain
3d printed foulingresistant photoactive membranes for wastewater treatment

conventional wastewater treatment plants wwtps are not effective in removing substances from our daily basis acting as the main release point of contaminants of emerging concerns cecs antibioticresistant bacteria arb and antibiotic resistance genes args to the environment aligned with sustainable development goals recently the european commission approved minimum requirements at the european level to reuse safely treated wastewater as an alternative source for irrigation to cope with water scarcity puraqua aims to enhance wastewater treatment efficiency at a lower cost by developing photoactive membranes with antifouling properties through additive manufacturing that can be applied as a posttreatment process after the conventional wwtps this challenge will be tackled by i producing new highly active and stable photoactive catalysts ii preparing antifouling membranes by addictive manufacturing and iii designing and developing a prototype reactor for wastewater treatment at continuous flow the proper design and fabrication of 3d printed photoactive membranes will allow the generation of reactive species at the membrane surface for the oxidation of persistent pollutants enhancing the water permeability at higher and more constant permeate flux with an impact on the treatment cost
singlefibre based holographic endoscope for observations of stroke in deep brain structure

the origin project lifegate introduced the prospect of multimode fibre based holographic endoscope as a unique tool for highresolution invivo imaging of structures residing deep inside the brain of animal models it harnesses the power of controlled light transport through hairthin mmfs to image and measure brain functions at single cell and subcellular level in virtually any brain structure at unlimited depth and in uniquely atraumatic manner thereby unlocking a variety of opportunities when answering urgent biomedical questionsthe proposed ercpoc project strokegate aims to utilise achievements of the origin project to introduce a novel complete technological platform for investigating the impact of deepbrain stroke in living animal models the geometry of the most advanced holographic endoscope currently built will be modified to allow induction of stroke of controlled magnitude representing a powerful extension of the instruments applicability from the perspective of the users in neuroscience already available imaging capacity of the instrument will facilitate its navigation into the desired brain structure and once the stroke is induced monitoring changes to structural connectivity of the neurones within a circuit as well as development suppression of signalling activitylifegate has already established a solid pathway to commercialisation through a startup endeavour although intellectual property is expected to be generated during the execution of strokegate its main translational value lies in demonstrating the utility of mmf endoscopes across the relevant community of researchers who by now had only limited chances to fully appreciate the potential of such radically new and disruptive technological arrival successful accomplishment of strokegate will unlock the exploitability of holographic endoscopy across a wider topical range of biomedical research and reduce its translational barriers
global knowledge exchange centre gkec for urban climate neutrality

the ambition of the global knowledge exchange centre gkec for urban climate neutrality is to mainstream actions towards climate neutral and netzero urban developments on a global level in this sense the gkec will provide support in terms of global outreach for the european cities mission as well as contribute to the urban transition mission of mission innovation it will provide effective support and knowledge for local governments and international stakeholders and through this support european policy such as the european green deal and work towards the international commitments of the paris agreementthe gkec will make available evidence and good practice for urban climate neutral and netzero transitions globally and facilitate capacity building knowledge exchange and moderated learning for municipalities and key stakeholders in operational terms the gkec will set up actions according to the three impact pathways1mainstream knowledge and good practice to accelerate urban transitions the mission platform will be extended towards global use making available international solutions and good practices and promote financing and funding opportunities to implement integrated urban climate action and netzero pathways2offer advanced frameworks and moderated learning across all governance levels to raise awareness of and capacities for cn and nz policies policy advocacy for climate neutral and netzero approaches will allow aligning of global policies capacity building measures for local and regional actors will provide guidelines and support mutual learning to drive systemic change 3establish the gkec as a forum for international exchange and cooperation on climate neutral and netzero transitions global and regional events accompanied by comprehensive dissemination measures will raise visibility of eu and international good practice and strengthen international cooperation on climate neutrality and netzero pathways
singleshot high repetition rate detection of the carrierenvelopephase of ultrashort laser pulses

the interaction of short laser pulses with matter often depends on the carrierenvelopephase cep which is the offset between the maximum of the electric field and the envelope of the pulse for highrepetitionrate laser systems this phase can usually only be measured as an average quantity we propose a novel analog method to measure the cep of ultrashort laser pulses in singleshot and for every laser shot at high repetition rate hundreds of khz to mhz while conventional approaches use slow array detectors and computationally expensive calculations we perform the measurement via optical fourier transform virtually not imposing any speed limitations the approach also opens up for new avenues for cepstabilization possibly making most of todays complicated and expensive stabilization equipment obsolete a patent application securing our ip has been submitted the project will be conducted together with our longstanding commercial partner sphere ultrafast photonics sphere has a strong position on the ultrafast laser market defining an efficient path for commercialization we plan to develop two different kinds of cep detectors ie the analog detector and a simplified digital detector working up to 100 khz for less demanding applications the detectors will be extensively tested and benchmarked against each other with a number of ultrafast laser sources in lund and at spherethe original idea for analog cep detection was inspired by our research in attosecond science supported by the erc advanced grant qpap quantum physics with attosecond light pulses 884900 which requires cep control the lack of existing techniques for the challenging repetition rate of our laser source 200 khz stimulated the ideas presented in this proposal which we now would like to explore as a proofofconcept
ebrains preparatory phase

this esfri preparation phase project will place the ebrains research infrastructure ri developed by the human brain project hbp on a sustainable footing the new organisational framework will allow ebrains to evolve from the largely ecfunded hbpdeveloped ri into a sustainable hubandnode ri on the esfri roadmapthe operation phase ebrains ri will serve the whole brain research community rather than the needs of a subset of users within that community this will be reflected in a broader offering of tools and services by the ri the operation phase ri will focus more on the processing managing and sharing of data of all sorts even those generated outside the ri but will retain an important role in providing cuttingedge datagenerating tools and services while staying true to its roots in supporting basic research it will expand its activities to support clinical research and innovative exploitation of brainrelated technologies the evolving ri offering will be determined with the help of a new governance structure which focuses on determining the priority needs of the broad brain research community the operation phase ebrains will be funded primarily by longterm commitments by the eu member states and associated countries that are home to a node in the ebrains riobjective 1 to put in place a financial legal and governance framework for ebrains and prepare a plan to ensure the sustainable offering of the service capability by the central hub and the nodesobjective 2 to obtain the legally binding commitment of ebrains consortium partners to offer services needed by the brain research community by setting up ebrains nodesobjective 3 to deliver a complete overall technical design and associated cost estimate for the service capabilityobjective 4 to decide the legal structure that ebrains will adopt for the operation phase and produce draft statutes for ebrains in that structure
fuel the brain in healtthy aging and agerelated diseases

the worldwide population is ageing and putting our healthcare systems under pressure to cope with agerelated diseases in europe there is a need for innovation knowledge and experts who can prompt significant changes to improve prevention and innovative interventional strategies to counteract altered metabolism in brain during ageing and related disorders the priority is to ensure brain health throughout life and during ageing increased collaboration between academia and private sector is the required milieu to overcome this challengethe fuel the brain in healtthy aging and agerelated diseases eternity will contribute to the strengthening of the european innovation capacity by training a new generation of researchers with experience and full understanding of the requirements of academia pharmaceutical and food supplement companies and the societyeternity research programme will focus on brain metabolism as a highly promising yet underexplored area given the complementary expertise eternity will have excellent opportunities to delineate different pathways underlying impaired brain metabolism to identify novel pharmaceutical targets andor nutritional interventional strategies to counteract ageing and related disordersthe earlystage researchers dcs will be trained in this environment and provided with a mindset of future commercial and clinical utilization of their research findings apart from the innovative and collaborative approach of the research programme the dcs will also be provided with a training programme where cuttingedge methodology innovation and transferable skills are key componentsthe trained dcs will have excellent intersectoral and interdisciplinary career opportunities and will together with the eternity partners provide a solid ground to tackle one of the major societal challenges of our century finding therapies to promote brain healthy ageing and delay the onset of neurodegenerative diseases
evaluation and validation of connected mobility in real open systems beyond 5gs

5g adv and 6g aim to expand the set of supported verticals and provide enhanced capabilities beyond connectivity 5g cam vertical services are a broad range of services in and around vehicles including both safetyrelated and other services enabled or supported by 5g 5gs has been built as a modular architecture to support any vertical running on top in a verticalagnostic manner however it is realized that certain verticals like cam have specific and strict requirements although significant progress has been made in supporting verticals the corresponding necessary configuration of the network and enddevices is a timeconsuming manual process that requires tight coordination at technical and business levels across the verticals the vendors the network operator and even the endusers this hinders not only the greater adoption of 5gs but also the uptake of novel cam ucs and the modernization of existing ones that require a tighter integration with the underlying network the main objective of envelope is to advance and open up the reference 5g adv architecture and also to transform it into a verticaloriented with the necessary interfaces tailored to the cam ucs that i expose network capabilities to verticals ii provide verticalinformation to the network iii enable verticals to dynamically request and modify certain network aspects in an open transparent and easy to use semiautomated way envelope aims to deliver 3 largescale b5g trial sites in italy netherlands and greece for cam services and beyond implementing functionalities tailored to the cam services and advanced exposure capabilities although focused on the cam vertical the resulting developments will be reusable by any vertical the envelope architecture will serve as an envelope that can cover accommodate and support any type of vertical services the applicability of envelope capabilities will be demonstrated via the project cam ucs and via at least 9 open call projects
singlecell and liveimaging approaches to study intratumor heterogeneity and population dynamics in colorectal cancer organoids

colorectal cancer crc is one of the most diagnosed cancer worldwide though several treatment strategies are available today around a million people die of this disease each year worldwide thus there is still a pivotal need for new and more effective treatments intratumor cellular heterogeneity has emerged lately as an intrinsic feature of malignancies and as one of the factors responsible for therapy resistance or tolerance however the role that phenotypically distinct subpopulations have on tumor biology is far from being understood this multidisciplinary proposal is based on the use of cuttingedge techniques to unravel the basic features of intratumor phenotypic heterogeneity and subpopulations interactions the goals are to 1 identify and characterize phenotypically distinct subpopulations on a set of metastatic crc humanderived organoids by singlecell rna sequencing 2 monitor their plasticity transitions by generation of fluorescent reporter lines and liveimaging lineage tracing 3 study the impact of each subpopulation on tumor growth and identify underlying molecular pathways by liveimaging ambitiously this project aims at witnessing phenotypic transitions in living cells a challenging task that has not been fully accomplished yet with relevant therapeutic consequences it is expected that this project will provide the scientific community with a deeper understanding on subpopulations dynamics in colorectal cancer that could ultimately result in the discovery of novel treatment strategies such as targeting a specific subpopulation or interfering with molecular determinants of transitions moreover it will foster the independent career of the applicant as a researcher by expanding her skills and international network this project tackles a relevant problem of modern cancer biology by employing innovative techniques complemented with stateoftheart facilities and high quality training and mentoring