Dragon09/odtu_chatbot huggingface dataset:
hydride hydrogenase driven h2 production through design and evolution

climate change is shaping up to be the greatest existential threat that humanity has ever faced to combat climate change and the corresponding energy crisis greenhouse gas emissions must be substantially reduced by 2030 while developing nonfossil future fuel alternatives such as hydrogen hydrogen is highly versatile as it can be used in both fuel cells and electricity production however currently 95 of the global hydrogen production is fossil fuelbased and not sustainable biotechnological hydrogen production is realized through microorganisms harbouring hydrogenases and represents a promising alternative to expand the proportion of sustainable hydrogen within the global budget however the utilization of these enzymes is limited by various mechanisms including inhibition by oxygen making biohydrogen in its current state not economically sustainablehydride is an interdisciplinary study aiming to overcome the oxygen sensitivity of fefe hydrogenases by designing and evolving highperformance hydrogenase enzymes this will be achieved by 2 major steps 1 using sequence data ancestral enzymatic scaffolds aec of fefehydrogenases will be designed and characterized towards their ability to produce hydrogen their oxygen sensitivity and active site characteristics aecs have been shown to provide good starting points for laboratory evolution thus these aces will be 2 evolved to overcome low hydrogen production under oxygen exposure using a highthroughput selection system consequently hydride will tackle one of the major bottlenecks for the utilization of fefehydrogenases paving the way for sustainable and economical biotechnological hydrogen production while addressing two societal un sustainability goals the knowledge gained from this study will substantially advance other scientific fields aiding eg the development and design of artificial catalysts
pointofcare devices for urgent care triage

cardiorespiratory diseases and stroke are among the top four leading causes of death in the eu in prehospital settings urgent care services are in huge need of novel point of care poc computing technologies that can specifically detect patient condition and enable hospital information system his with realtime data for triaging patients for right care our goal in poc4triage is to develop robust and accurate poc technologies from poc testing devices to poc systems platform that is capable of fast diagnosis and efficient transfer of data to his we will develop and clinically validate four rapid 10 min easytouse compact cost and energyefficient poc devices with edge ai computing models to be used in ambulance emergency room settings poc4triage devices include a multimodal patch for realtime monitoring of cardiorespiratory data novel subhairline noninvasive eeg based head caps for rapid stroke diagnosis including detection of large vessel occlusion stroke and a handheld rapid immunodetector to diagnose stroke with clinical utility for various conditions these devices integrate into a new device hospital connectivity platform dhcp that visualizes data uses ai from multiple devices to triage and seamlessly integrates with hospital systems and clinical workflows the poc devices and dhcp will be clinically validated poc4triage brings together some of europes leading poc device developers medical professionals and clinicians patient representatives ethics experts data scientists and health economists poc4triage will shorten the time to treatment and improve clinical outcome poc4triage has potential to revolutionize healthcare delivery making it more accessible and efficient traceable and interpretable for patients and providers alike as the poc device and computation market is growing fast the new poc devices realtime data analysis and secure computing have potential for major economic impact
microbial synthetic in vivo cell therapy systems

bacteria are among the most brilliant chemists on earth they are capable of producing a wealth of structurally diverse natural products with a wide range of applications in medicine such as the treatment of infections and cancer the synthetic production of microbial natural products and their transformation into pharmaceutical drugs is often a challenging costly and timeconsuming process due to the structural complexity of these molecules and the difficulties associated with drug solubility and formulationan emerging new strategy in disease treatment aims to exploit beneficial intestinal microbes for local drug production and delivery such commensal bacteria are safe can be administered easily and can be engineered to detect diseases and release drugs in adequate local concentrations however the current therapeutic platform or chassis strains cannot stably colonize the human gut and have so far only been engineered to produce therapeutic proteins such as hormones or cytokines as a result no commensal chassis strains are available to treat chronic intestinal diseases or to produce clinically important natural product therapeuticsin mistic i propose to revolutionize microbial therapy systems by developing the beneficial stable and prominent gut colonizer clostridium leptum as an innovative and superior chassis for longterm health monitoring and chronic disease treatment i will combine my expertise in molecular microbiology enzyme engineering and natural product biosynthesis to equip the chassis with a nanobodybased biosensor and biocontainment modules and optimize it for the expression of natural product biosynthetic pathways as a proofofconcept we will implement these tools and chassis strains for the detection and treatment of colorectal cancertogether these innovations will have broad translational applications and will pave the way to a new frontier in the field of microbiome engineering and synthetic microbial therapy systems
the role of lasp1 and pdlim4 in stress fiber dynamics and mechanoresponsive signaling

the lim domain proteins lasp1 and pdlim4 are associated with the actin cytoskeleton and implicated in many diseases however their precise molecular functions are poorly understood recent protein interaction studies from my host laboratory provided evidence that these two proteins associate with stress fibers sfs the contractile actomyosin structures of nonmuscle cells distant homologues of lasp1 and pdlim4 are also present in muscle sarcomeres suggesting a potential structural role of these proteins in sfs however several other lim domain proteins are involved in a widevariety proteinprotein interactions and signaling pathways suggesting possible roles for lasp1 and pdlim4 in signal transduction especially in mediating mechanosensing in sfs indeed previous studies proposed that lasp1 is a possible regulator of translation whereas pdlim4 may function as an adaptor between the cytoskeleton and kinases the aim of my work is to elucidate the molecular functions of pdlim4 and lasp1 using a combination of proteomics cell biological genetic and biochemical approaches my specific interest is to uncover whether pdlim4 and lasp1 are structural components of stress fibers or if they function as mechanosensitive regulators of translation or intracellular signaling the findings will uncover the roles of pdlim4 and lasp1 in cytoskeleton organization potentially providing new insights into various diseases including cancer additionally understanding the interplay between sfmediated mechanosensing and translation may reveal new mechanisms by which cells respond to mechanical cues overall this study will broaden our understanding of sfsignaling and has potential implications for therapeutic interventions in diseases linked to sfdysregulation
learning hub as a digital platform for augmented innovation and assessment

the european innovation council eic has created a new wave of opportunities to support and fund european deeptech breakthrough projects thanks to ambitious eic accelerator program and a unique eic ai platform a european ecosystem of stakeholders is tasked to help assist and guide innovators through their applications journey the lightroom project holds the ambition to improve the capacity building of a targeted group of stakeholders through an augmented understanding and learning experience of the objectives triggered by the eic accelerator program as well as a better control of functionalities and methodologies implemented in the eic ai platform used by applicants in achieving so stakeholders will be capable to provide highquality assistance to applicants in their command of the different processes underpinned in the eic ai platform as well as sustaining the dialogue between applicants and the eic through proven methodologies used in the eic ai platform lightroom will engage in a continuous interaction with the different stakeholders to contextualize knowledge gaps and needs leading to personalized training offers empowered by the artificial intelligence this will enable creating custommade training development plans that would best align to individual profiles and respond to knowledge gaps assessed through digital assessment tools lightroom will develop and provide a unique digital training platform enriched with practical reallife use cases and an interactive user experience through specific information and knowledge that will help anchoring conceptual and technical understanding and then the control over the usual application process to achieve so lightroom will provide a smooth mix of physical and digital training experience making sure progress are continuously monitored all along the project lifetime
making the invisible visible for offhighway machinery by conveying extended reality technologies

theiaxr making the invisible visible for offhighway machinery by conveying extended reality technologies aims at improving humanmachine interaction in mobile machinery by enhancing user technology fit and extending reality technologies and functionalities the results will make the invisible visible respectively the nonperceivable perceivable to the human operator extending the perceivable range of the operator without negatively influencing the performance of the human while controlling the machinery the project will ensure positive impact of extended reality technologies on the safety security trustworthiness and societal aspects of the interaction between the machine the human operator and publicnonusers and furthermore create an xr workplace that enables positive user experiences of selfefficacy and meaningfulness of worktheiaxr will leverage a humancentred transdisciplinary and scenariobased codesign methodology with users stakeholders and multidisciplinary researchers as a fully integrated project team this methodology serves to collect human requirements and to design develop and deploy the extended reality technologies for information presentation and interaction in the offhighway domain the targeted extended reality technologies will enhance conventional humanmachine interfaces through multimodal information and interaction technologies deploying innovative data visualization methods force feedback technology and acoustic informationthe theiaxr approach for improving the human operator tasks by deploying extended reality technologies will be initially validated and tested in three uses cases in the offhighway domain targeting snow grooming logistics and construction scenarios integrating real endusers publicnonusers and reallife data from dedicated industrial environments
in vitro and incell characterization of quadruplexduplex hybrids conformation folding and recognition by druglike ligand molecule

nucleic acids are intrinsically polymorphic apart from the watsoncrick duplex guaninerich sequences can form gquadruplex g4 structures which have become attractive targets for small molecule ligands it is now proven that gquadruplexes can form in cells today most biophysical and structuralligand binding studies are carried out in dilute aqueous solutions and the presence of artificial crowding agentscosolvents still recent works suggest that the folding of g4s may differ in the cellular milieu context due to the agents limitations thus there is an immense need for incellbased structural biology approaches in the proposal i aim to address critical milestones leading to the bioactive conformation inside the cell and the development of effectivespecific g4 targeting drugs using an essential subclass of g4 structures as a paradigm namely quadruplexduplex hybrids qdhhereafter we will focus on low and highresolution spectroscopic approaches and mass spectrometrybased ligand screening assays characterizing ligandqdh interaction on the methodological part we will develop incell nmr in native conditions starting from unfolded conformation denovo folding and propose a ligand screening assay inside the cellular system our project will contribute to unveil fundamental principles of nucleic acid folding it will also foster collaboration between two european institutes specialized in complementary biophysical and structural approaches to study biomolecular folding in a nutshell the maria skodowska curie fellowship will flourish my scientific excellence and the resilience needed for my original ideas to become a reality and communication and public engagement skills across cultures and disciplines
fastresponse electrically heated catalytic reactor technology for co2 reduction

the ecoduct project aims at electrifying the simultaneous chemical conversion acid gas components co2 and h2s into the platform molecule co and marketable sulphur using an electrothermal fluidised bed reactor etfb technology the corresponding process will comprise two steps a first one for co2 and h2s reduction into cos and a second step for cos decomposition into the platform molecule co and claus grade sulphur to demonstrate its valorisation potential co will be converted into green methanol as final product using a third reaction the ecoduct consortium is composed of nine entities including industrial and academic partners spanning a complete value chain from material suppliers and engineers to modelling experts and technology providers ecoduct will optimise and scaleup the reactor materials and catalysts to trl6 to 16ty of co production while reducing reactor size by 50 among others via the removal of heating units technoeconomic and environmental assessment of the reactor performances will demonstrate 40 capex and opex as well as 50 of ghg emissions optimisation of operating conditions and reaction yield will be supported by fundamental microkinetic modelling as well as industrial process planning accounting for variability of feedstock composition and renewable energy resources integrated conceptual design will fasten future scaleup and commercialisation of the reactor demonstrator at trl9 with a final capacity of 34kt of co2 converted per year ecoduct will provide a firstofakind fastresponse electrically heated catalytic reactor able to replace the conventional claus unit for sulphur recovery and simultaneous electroreduction of co2 allowing 50 energy demand for acid gas treatment in over 130 refineries in europe by 2035 the process could then be diversified to other applications such as fcc steam cracking and dehydrogenation and multiple sites as biogas digesters and gas plants representing 18000 sites in europe
liquid dosimetry via electron paramagnetic resonance spectroscopy

the problem lack of waterequivalent dosimeters for radiotherapycancer is a global public health problem radiotherapy is generally used as an essential method of cancer treatment measuring the absorbed dose of radiation to ensure that the radiation delivered to the patient is accurate is paramount and critical to patient safety since tumor control and sideeffects are highly sensitive to the applied dose for this purpose invivo dosimetry ivd can be performed as a patientspecific measure of quality control and safety during radiotherapy clinical experience and dose prescriptions in radiotherapy have historically been based on the absorbed dose to water however when a calibrated dosimeter as for today which is not water equivalent is used under nonreference conditions direct determination of the correct absorbed dose to water is not possible and many detectors show an overresponse water equivalent dosimeters are not available but particularly desirable in clinical applications the solution a small waterequivalent nontoxic and liquid dosimeter with electron paramagnetic resonance spectroscopy readoutthis idea presents an innovative strategy for dose determination using a novel type of liquid waterequivalent and nontoxic dosimeter with readout via electron paramagnetic resonance epr spectroscopy this novel liquid epr dosimeter consists of a small mmdimensions capsuled waterbased liquid detector material that can be placed on the patients skin or in body cavities to measure the delivered dose at relevant locations in clinical situations the principle presented here is based on a dosedependent epr signal loss stable paramagnetic species dissolved in aqueous solution are transformed into diamagnetic products upon irradiation the proposed solution is not an incremental improvement of an existing procedure but an innovative approach making ivd easier to use cheaper more accurate and water equivalent
feminist movements responses to the gendered impact of crises gender resilience and activism in crisis europe

the gendered effects of the current pandemic crisis ie how the economic social and political problems prompted by the covid19 health crisis affect women men lesbian gay bisexual transgender and intersex people differently are amongst the most visible and significant ones yet continue to be largely underestimated in the mainstream political policy and academic debates women have and will continue to endure the social economic and political damage generated by the crisis due to precarious work conditions as well as unpaid care and housework in this context feminist movements have been capable to reintroduce contentious claims over the deep meaning of the crisis and its consequences raising public concern over imperative issues such as genderbased violence reproductive rights and the centrality of care and social reproduction work as the material basis to sustain life against the backdrop grace investigates how feminist movements address the gendered consequences of the crisis by looking at the variation in mobilization opportunities strategies and policy outcomes in two national cases italy and spain it does so by examining feminist movements in a online and offline strategic choices tactical innovation and changes in mobilization style b forms of reorganization in the physical and digital space c discourses and knowledge production adopted to influence institutional actors and public opinion based on a mixmethods and crossnational comparative research design grace aims to enhance the understanding of gender inequalities across contexts crises consequences and the influence of feminist movements on public policy in doing so grace will produce crucial knowledge to academics and policy makers to understand how the current crisis management entrenches the power of particular economic and social orders enables structures and mechanisms of gender privilege and multiple inequalities
tracing galaxy evolution with nuclear structures in latetype galaxies

central regions of galaxies are inhabited by dense small structures such as nuclear star clusters nscs and nuclear disks and rings these at the bottom of the galactic potential well are important tracers of the overall galaxy evolution but the dominant mechanisms of their formation in galaxies of different masses and morphologies are still unclear the translate project tracing galaxy evolution with nuclear structures in latetype galaxies will shed light on this issue combining highresolution zoomin cosmological simulations with stateoftheart integralfield spectroscopy observations translate will be conclusive on the role of gas accretion and inflow followed by nuclear insitu star formation and stellar accretion and migration to the center of a galaxy first i will quantify the relative contribution of these processes in the central regions of 50 simulated galaxies i will identify potential nuclear structures and unveil how they formed going back in time to previous snapshots of simulations secondly i will focus on nscs in observations of eight massive latetype galaxies so far poorly studied and their properties will be interpreted with the help of recipes provided by simulations finally since higher resolution than current state of the art is needed to detect the smallest nscs translate will deliver one nscoriented pilot simulation with a factor of 10 higher resolution and a detailed plan for a future complete run of 20 more simulations the translate project will be carried out at the instituto de astrofsica de canarias under the supervision of dr c brook expert on theoretical studies on the formation of galactic structures in a cosmological context the theoretical expertise of the supervisor and the host research group is very complementary to my observational background and with this fellowship i will acquire a complete versatile and mature profile as a scientist in a position to pursue longterm leadership positions
humanenvironment interactions in and around venice lagoon microecologies and geoarchaeological narratives from roman period to middle ages

waterscapes is an interdisciplinary project that examines the relationship between environmental changes natural constraints and potentialities and past societies settled in and around venice lagoon from the roman to medieval periods waterscapes has 3 core objectives first it will explore the highresolution potential of paleoarchives which have been undervalued by traditional archaeological research and textbased narratives this will be achieved through geophysical surveys and coring missions in various sites venice torcello altinum lio piccolo aquileia grado laboratory research geochemistry biosedimentology etc and contextual analysis of the settlements through literature reviews second it will examine waterscapes as a humanmade environment in venice lagoon in order to determine the historical roots and mechanisms responsible for the holoceneanthropocene transition the research will be centred on multiperiod waterscape archaeology to see how former cultures shaped themselves to the surrounding environments and developed different waterscapes through water management practices waterscapes will qualitatively and quantitatively analyse the degree and consequences of human interventions vs natural dynamics using multivariate statistical analysis based on biogeoarchaeological proxies from the investigated sites third waterscapes will emphasise the social aspect of the materiality of the past simultaneously measuring its legacy in the present establishing the relation between variations in past climatic conditions and coastal changes enables to contribute to the understanding and modelling of the ongoing processes thereby providing tools for the policies of territory management waterscapes integrates perfectly in the principles of european framework for action on cultural heritage and in two areas of continued actions a resilient europesafeguarding endangered heritage and an innovative europemobilising knowledge and research