Dragon09/odtu_chatbot huggingface dataset:
nextgeneration fungicides for crop protection based on artificial small rnas derived from viral vectors

plants are threatened by a plethora of pathogens with fungi accounting for important crop and postharvest losses worldwide despite widespread spraying of fungicides losses keep rising in the current warming world and the increasing use of chemical and toxic fungicides favours the emergence of resistant fungi strains hence there is an urgent need to develop a new generation of highly efficient and environmentalfriendly fungicides for securing present and future food availabilityclassic rna interference rnai tools based on doublestranded rna have been used for inducing antifungal resistance in plants despite their lack of high specificity more modern and highly specific rnai tools are based on artificial small rnas artsrnas artsrna tools have been optimized mainly for high efficacy and transgenic use but are not well adapted for gmofree application and have not been used yet for inducing antifungal resistance in plants funsynvigs seeks to develop a novel gmofree rnai technology consisting in systemic viral vectorbased fungicides expressing artsrnas for plant immunization against pathogenic fungi the specific objectives are1 the development and optimization of viral vectorbased fungicides expressing artsrnas against botrytis cinerea the causal agent of the gray mold disease in the model plant species nicotiana benthamiana2 the application of viral vectorbased fungicides for immunizing tomato plants against b cinereasuccessful completion of these objectives will provide a novel class of mobile rnaibased fungicides for inducing highly efficient antifungal resistance in crops
selfmonitoring of prostate cancer by automated hybrid recognition of exosomal mirna toward personalized analytical tool

tumorderived circulating exosomes are a suitable readily accessible reservoir of cancer biomarkers for disease process analysis the micrornas mirnas inside the exosomes provide information about the mechanisms of change in cancer of affected individuals quantitative determination of mirnas in the exosome a clinical area of unmet need is tempting but challenging the main objectives of the proposal are to develop a noninvasive affordable userfriendly and deliverable platform that can measure mutant mirna107 in prostate cancer pcaassociated exosomes with unmatched accuracy combining a hybrid oligonucleotides onsnanomaterials integrated composite system with an epad the proposed platform offers selfmonitoring of prostate cancer by automated hybrid recognition of exosomal mirna towards personalized analytical tool named smart smart provides a universal technology for noninvasive pca diagnosis and quantitative tracking of mirna levels in cancerderived exosomes in a very simple and effortless manner in biofluids such as serum and urine the novelty of the proposed interdisciplinary project work will evolve into the next generation of intelligent pointofcare poc tools for precision medicine this proposal will contribute to the sustainability of the healthcare system by enabling patients to stratify andor predict disease thereby improving clinical decision making this msca pf will be carried out at the university of naples italy will provide the researcher with advanced technical training in exosome isolation pcr and dna sequencing molecular biology microfluidics pathology nanomaterials synthesis analytical techniques proposal and manuscript preparation and academic supervision in research management the proposed multidisciplinary collaboration in the smart project has resulted in a new generation of smart medical devices with functions comparable to existing highly specialized hospital devices
from fragments to high affinity binders interfacing integrated structural biology medicinal chemistry and artificial intelligence

fragmentscreen will develop innovative instrumentation workflows and experimental and computational methodologies for fragmentbased drug discovery fbdd enabling access to early phase structurebased drug discovery for all biological targets and for scientists both in industry and academia the established workflows will use structural biology insights and associated data to feed artificial intelligence ai methodology to guide medicinal chemistry in drug development fragmentscreen brings together scientists from four esfri landmark research infrastructures ris esrf the european synchrotron and the distributed ris euopenscreen eric medicinal chemistry elixir data resources for life science under the coordination of instructeric integrated structural biology and seven industry partners in scientific instrumentation and computational and ai sectors including smes to remove crucial bottlenecks in early phase drug discovery the new instrumentation and workflows will be available at european ris while the new instruments will be commercialised to increase the technological competitiveness of european industry in drug design and the attractiveness of structural biology ris for the pharmaceutical and biotech sectors fragmentscreen will implement open science approaches in early drug discovery to maximise the impact of screening campaigns generated data will be made accessible so that iterative cycles applying ai will improve response times for drug discovery we will create a framework for the objective and independent evaluation of ai in drug design to identify critical developments in this thriving research field methodologies for rigorous crossvalidation will be established through demonstrator and pilot studies and results will be disseminated to the large scientific communities affiliated to the european ris and beyond
sustainable and green electronics for circular economy

the eu requires also electronics industry to achieve the ambitious goals of the eu green deal circular economy action plan and industrial strategy for reduction of energy and material consumption and utilization of circular value chains sustronics project is targeting to improve capabilities of european electronics industry to meet these goals and develop new business opportunities from sustainable and greener electronics combined with increase in productivity and new functionalitiesthe current electronic industry poses significant environmental impacts such as increasing amount of ewaste great demand for critical raw materials and high energy consumption during manufacturing electronics industry can specifically decrease its environmental burden by shifting from fossilbased materials to biobased materials decreasing use of metals utilizing additive manufacturing processes and developing miniaturized and integrated components but also in broader scope by utilizing efficient circular economy business models that enable reuse recycle and repair of critical materials and componentssustronics main goal is to support renewal of european electronics industry towards circular economy ecodesign biobased materials and material and energyefficient manufacturing processes thereby sustronics will redesign electronics products into circular compostable and reusable products and demonstrate that there are business opportunities in sustainable electronicsquantification of environmental impact definition of business models involvement of external stakeholders and means to guarantee compatibility with policies and standards will guide the project implementation the pilots will focus on healthcare diagnostics and industrial sectors including topics such as medical and personal health devices singleuse and wearable diagnostics sustainable lighting solutions and embedded electronics for automotive
battery management system and system design for stationary energy storage with 2nd life batteries

battery2life will facilitate the smooth transition of batteries to 2nd life use and boost the innovation of the european battery industry by providing enablers to implement open adaptable smart bmses and improved system designs and proposing methods for the efficient and reliable reconfiguration of used batteries battery2life introduces two new battery system design frameworks serving the upcoming market needs the first supports the business transition for the initial market by restructuring existing battery design patterns while the second one introduces completely new design principles for 1st and 2nd life of the battery a completely new bms design mentality is introduced to the battery industry by delivering an open and interoperable hybrid bms architecture with an embedded and a cloud section leading the transition from technologydriven bms designs to serve the needs of specific applications and battery technologies to new datadriven and applicationagnostic bms designs that can be easily adapted and updated to serve the requirements of different battery technologies and any 2nd life battery stationary storage application furthermore battery2life introduces innovative embedded sensing and more accurate sox estimation algorithms new sox indicators appropriate for 2nd life use ie sos safety and sow warranty and a new eis implementation approach by integrating it in the bms that will enable the detailed safety and reliability monitoring at both cell and module level during 1st and 2nd life usage the project will specify an open bms concept data formats taking into account and extending the battery passport concept and interoperable communication via the cloud platform to third parties including the future passport exchange system to facilitate monitoring and assessment the project prototypes will be demonstrated within the context of two business cases ie domestic storage application and utilityscale load levelling one
quantum software platform for multiphysics simulations

this project will develop qucoflow the worlds first hybrid quantumai software platform for multiphysics simulations qucoflow integrates the most advanced simulation algorithms and deploys them to accelerate europes transition towards sustainable mobility qucoflow will be the first software capable of performing realistic batteryfuel cell performance and degradation and aerodynamics simulations further it can be seamlessly integrated into standard simulation software by leading vendors
dynamics of crossover designation

meiotic crossovers make us unique their distribution along chromosomes dictates which traits will be reassorted to create new and unique allele combinations on which selection can act early during meiosis a large number of recombination interactions are initiated all across the genome but only a few ultimately mature into crossovers the final number and positions of crossovers are tightly regulated along each chromosome crossovers tend to be evenly spaced this phenomenon called crossover interference was discovered in 1914 by sturtevant and morgan while drawing the first recombination map in flies emergence of spatial patterning requires communication but how does the crossover formation machinery communicate with neighboring crossovers half a chromosome away our understanding of how this communication is established has been hindered by the limited resolution in time provided by classical cytology giving us access to only snapshots of the process in the dynaco project i will develop innovative solutions to explore the dynamics of crossover designation and interference using gentle live superresolution microscopy combined with groundbreaking genetic and optogenetic tools in a very amenable system the filamentous fungus sordaria macrospora i will address the following questions i how do procrossover factors behave dynamically to enact and respond to crossover interference ii what is the medium supporting crossover communication iii what are the consequences of locally disrupting this communicationthe dynaco project will provide fundamental breakthroughs in our understanding of crossover formation designation and interference we will confront and reconcile many aspects of current models for crossover interference and develop our own unified model this work also has the potential to provide tools for the manipulation of recombination to accelerate the introgression of selective traits into elite crop genomes
validating biodegradation rates and reactions applying novel technologies and systems ecology approaches

plastic marine debris pmd is now considered a planetary boundary threat and the plastic cycle a biogeochemical cycle the impacts of singleuse plastic litter in marine environments have prompted a search for greener alternatives such as the biodegradablecompostable plastics polyhydroxyalkanoates and polylactic acid already being used for consumer goods the diversity of microbes their enzymes and their role in influencing the rates of plastic biodegradation in nature remains poorly characterized particularly in cold or lowoxygen marine waters and seafloor where most pmd accumulates the impact of lag duration ph hydrostatic pressure and potential priority effects of initial microbial colonizers remain vital but unaddressed concerns in addition the impact of plastic its breakdown products and additives on marine organisms and ecosystems is largely unquantified two urgent questions are how long does plastic last in the marine environment and what are the impacts on ecosystems this technologyenabled project aims to quantify biodegradation rates of biodegradable plastics and plasticassociated additives in the field and under controlled laboratory conditions and to develop a fish model system for toxicity tests to fill these knowledge gaps this project will generate novel microbial metagenomes from global plastic samples collected during cruises and from biodegradation experiments to create a comprehensive list of genes and enzymes linked to biodegradation of biodegradable plastic in marine environments it will be the first to combine in situ respirometry stable isotope probing and advanced imaging techniques to measure biodegradable plastic biodegradation rates while visualizing microbemicrobe interactions on the plastic surface revealing cellular mechanisms occurring during active biodegradation finally it will develop the first marine fish organoid model for consistent and efficient testing of toxicity due to plastics and additives
rise of the structureencoded unc5 interactome in aggressive behaviours of neurodevelopmental tumours

paediatric tumours represent a challenge in the landscape of cancer research unlike adult cancers they typically originate from embryonic cells from which they inherit characteristic embryonic features this is exemplified by neuroblastoma nb the most common solid tumour in young children nb is a heterogeneous cancer with aggressive metastatic forms which emerges from neural crest cells these cells normally undergo a complex migratory process that depends on the functions of cell surface signalling receptors in a recent groundbreaking study we found that nb cells coopt one specific family of these receptors unc5 and the ligand gpc3 to disseminate cell 2022 disruption of this interaction in vivo impacted on cell cohesion migratory properties and redirected tumour localisation here we provide unpublished data analysis suggesting that an entire unc5 interaction network involving multiple different ligands besides gpc3 is at play in malignant nb understanding the mechanisms driving nb cell aggressive behaviours requires investigation of this network in the embryonic context a key strength of the proposal is our unique approach bringing molecularstructural biology to in vivo experiments our strategy maximises the use of targeted structurebased perturbation experiments where we control the activity of individual receptor interaction precisely in vivo we also have the necessary access to patient samples and will use our innovative avian xenograft model which recapitulates the embryonic environment of nb in a final workpackage we will apply our strategy developed with a focus on nb to two other paediatric cancers where unc5 is expressed in sum the results of sunrise will produce a first detailed picture of the where when and how unc5 network interactions drive paediatric cancer biology and could lead to new therapeutics for example using nanobody technology
electrochemical borylation via borylene species bridging a gap in synthetic chemistry

carbenoids carbene and nitrene have revolutionized the field of organic chemistry reshaping and improving the molecular construction landscape and unveiling previously inaccessible reaction pathways this chemistry has massively simplified the synthesis of complex molecular structures such as pharmaceuticals agrochemicals and polymers in contrast with the essential role of carbenes and nitrenes the boron analogue borylene has received limited attention in synthetic chemistry a basic understanding of borylene reactivity has hindered its utilization in organic synthesis primarily because of its synthetically challenging formationthis proposal aims to harness electrochemistry as a novel synthetic approach for the controlled generation of borylenes this chemoselective platform will determine the critical factors that influence the reactivity of borylene intermediates and will enable the use of new borylation techniques previously out of reach of chemists having successfully demonstrated the first example of electrochemical borylene formation we anticipate the emergence of various novel borylation techniques among others i the synthesis of underexplored strained threemembered boracycles by cycloaddition reactivity unlocking access to novel classes of molecules and ii the activation of ubiquitous bonds through boron insertion into cc which will utilize unprecedented retrosynthetic logic for ring expansion and 1nsubstituted chemical motifs thus shortening the synthetic scheme of functional materialsdrawing inspiration from the remarkable impact of carbenoids across a multitude of chemistry domains we envision that the electrosynthesis of borylenes will empower chemists to employ these reactive species to unlock access to entirely underexplored classes of molecules and will provide profound insights into borylene reactivity consequently charting a captivating course towards undiscovered realms in chemical synthesis
ocean cryosphere exchanges in antarctica impacts on climate and the earth system

oceanice will assess the impacts of key antarctic ice sheet and southern ocean processes on planet earth via their influence on sea level rise deep water formation ocean circulation and climate an innovative and ambitious combination of observations and numerical models including coupled ice sheetclimate model development will be used to improve predictions of how changes in the antarctic and greenland ice sheets impact global climate it will make new circumpolar and atlantic observations in observational gaps it will assimilate these and existing data into improved ice sheet boundary conditions and forcing producing new estimates of ice sheet melt and impacts on ocean circulation including the atlantic meridional overturning circulation it will develop calibrate and assess models used to predict the future evolution of the giant ice sheets it will reduce the deep uncertainty in the impact of their melt on societally relevant environmental changes on decadal to multicentennial time scales oceanice will assess the potential for passing ice sheet tipping points and their consequences for ocean circulation and climate oceanice will raise the profile of european research through its extensive network of international collaborators who provide scientific and logistical support it will directly contribute to the allatlantic ocean research alliance through observations logistical collaboration and analysis it will significantly advance the stateoftheart in coupled ice sheetclimate modelling and directly contribute to international climate assessments such as the intergovernmental panel on climate change and world ocean assessment it will link organically to european data centres to disseminate its data following fair and inspire principles it will deliver improved assessments of european climate impacts from the melting ice sheets with actionable risk and timescales to policymakers and the public
printed computing enabling extremely low cost pervasive near sensor computing

a large number of important domains such as fast moving consumer goods and personalized medicine have still not seen the benefits of computing mainly due to high production costs of rigid silicon technologies printed electronics based on additive manufacturing processes holds promise of meeting cost and conformity needs of such applications however the realization of traditional digital processor architectures is infeasible due to constraints of lowcost manufacturing such as form factor low device count large feature sizes and high variations the fundamental research question hence is how to perform accurate reliable and energyefficient classification computing to meet target applications requirements within the constraints of additive printed manufacturingthe aim of pricom is to make breakthroughs by developing unconventional mixedsignal classifier computing paradigms together with their hardware realization and mapping based on additive printing technologies this enables to significantly reduce the hardware footprint and directly process analog sensory inputs while achieving high classification accuracy nevertheless it is a major challenge as analog computing is very sensitive to variations and at the same time additive manufacturing is inherently prune to printing variations i aim at closing this gap by 1 utilizing the inherent tolerance of neuromorphic computing to variations with special hardware primitive design and training algorithms 2 designing novel variationaware physical design algorithms and 3 developing an iterative tuning flow exploiting unique features of additive manufacturing the feasibility of multidisciplinary research of pricom is underpinned by my unique crosslayer expertise and will be tested by fabricationbased demonstration of printed computing systems pricom can enable proliferation of computing in consumer market and personalized medicine bringing economical gains and improving quality of life
policy evaluation of a pregnancy benefit as a novel form of family policy in the eu

the proposed action contributes to tackling of a pressing challenge faced by the eu which is finding new birth policies that would be effective in countering demographic decline it does so by evaluating the impact of a new type of family policy pregnancy benefit on fertility behaviour births and abortions and on child health at birth this is a unique research opportunity as pregnancy benefits are very rare the evaluation will be conducted using highquality populationwide administrative microdata and stateoftheart microeconometric modelsthe project is inherently interdisciplinary as it connects economics with demography public policy sociology and womens reproductive rights the action includes intersectoral transfer of knowledge first the secondment institution conducts policyrelevant research tailored for the needs of the government and other public bodies second the nonacademic placement will occur in an ngo promoting gender equality and womens rightsthe novelty of this research is threefold first it evaluates the impact of a novel prenatal cash transfer on fertility as opposed to traditional postnatal policies second it contributes to understanding of abortionseeking behaviour which is rarely done third it evaluates the impact of an earmarked nonmeanstested transfer on child health at birth which has not been done previouslythe proposed policy evaluation is important in the context of1 tackling demographic decline in the eu a threat to longterm sustainability of public finances2 supporting costeffective policymaking evaluating effectiveness of costly family policies and choosing the best ones is useful for the taxpayer3 fostering gender equality the evaluated antiabortion policy is not restricting womens reproductive rightsthis project will strengthen the era as it will be executed in a low ri performing country currently no economists in slovak academia specialize in family policies