Dragon09/odtu_chatbot huggingface dataset:
scalable microbial metabolite discovery through synthetic biology

the discoveries of microbial secondary metabolites sm led to an incalculable impact on human health and lifespan a large share of the antibiotics anticancers and immunosuppressants in use today originate from microorganisms however discoveries of sms of microbial origin through traditional approaches have declined in the past decades depriving drug pipelines from a key source of bioactive molecules the consequences are dire in particular in the case of antibiotics encouragingly the study of the biosynthetic genes responsible for the synthesis of sms indicates that the natural repertoire remains vastly underexplored and new ways to access it are enabled by dna technologies in particular untapped biosynthetic genes can be interrogated by transferring them into heterologous hosts i am developing a method for transfer which fulfils the conditions to unleash theoretically massive economies of scale here i propose to multiply the scalability of the current heterologous expression framework and effectively overcome the diminishing returns faced by traditional approaches in the first axis i will implement a fullfledged discovery platform optimized to systematically interrogate novel biosynthetic gene clusters identified bioinformatically from a large collection of soil bacteria second the scale and parallel nature of the heterologous expression setup will be used to better understand the logic of activation of biosynthetic genes and identify optimal ways to increase activation rates among the large reservoir of conditionally silent biosynthetic genes finally the general strategy will be extended to new fungal and bacterial clades to enable interrogation of their vast potential through scalable heterologous expressionoverall this proposal aims at developing heterologous expression into a major way to discover microbial secondary metabolites and drug leads
a sonogenetic brainmachine interface for neurosciences and visual restoration

brainmachine interfaces are classically based on multielectrode arrays optogenetics has revolutionized this ability to control neuronal activity through ectopic opsin expression but its application to large brains remains limited because of tissue light absorption and scattering ultrasound waves have been proposed for remote neuromodulation but this approach has remained restricted to lowfrequency excitation to prevent brain tissue overheating at the expense of spatial resolution the expression of mechanosensitive proteins in neurons has recently made it possible to achieve the ultrasound us activation of neurons at high us frequencies an approach named sonogenetic using the mscl mechanosensitive ionic channel we recently provided a first proof of concept in rodents that cortical neurons can be activated in vivo by ultrasounds at a high spatiotemporal resolution and low intensity here we propose to develop an integrated sonogenetic strategy for distant activationinhibition of neuronal networks by ultrasound waves in large primate brains the project includes 1 optimizing and diversifying the sonogenetics actuators for safe neuronal control 2 designing technologies for patterned ultrasound stimulations 3 demonstrating efficacy and high spatiotemporal resolution of the sonogenetic approach in controlling the primate visual cortex this project is based on a unique synergistic combination of expertise with a synthetic biologist in ion channel engineering a neurobiologist with expertise in vision and a physicist with expertise in ultrasound technologies these 3 pis will be supported by an expert in aav vector mediated delivery the project outcomes will be a novel brainmachine interface for investigating neural circuit function even deep within large brains through contactless sonogenetics this sonogenetic technology will open new avenues for treating neurological dysfunction such as restoring vision in blind patients
atomically precise nanoribbons quantum platform

atypiquals main objective is to unveil a room temperature quantum technology platform based on a novel generation of atomically precise graphene nanoribbons aprs and demonstrate its feasibility through technologically relevant devices by definition quantum technologies exploit the peculiar quantum properties of matter such as superposition tunneling and entanglement to develop tools and devices with new nonclassical functionalities however derived using conventional topdown techniques these technologies lack atomic precision and are much more sensitive to environmental disturbances as compared to the electrical current switching in transistors as such they often require ultralow temperature operation and impose important constraints on their general applicability this makes their manipulation and integration to chipscale substrates a major challenge atypiqual is a highly interdisciplinary project that proposes a radically different highrisk bottomup approach that uniquely offers to naturally hardwire complex quantum states into atomically precise carbon nanostructures aprs they exhibit novel physical properties beyond graphene such as topological quantum phases and spin polarization all tailorable by their topology and edge structure however the demonstration of these electronic properties requires atomic precision control that can only be achieved through recent advances in bottomup synthesis we shall exploit atomically precise bottomup onsurface synthesis to develop these novel advanced aprs offering ondemand multifunctionalities while relying on a single graphene nanoribbon backbone by developing the required material processing and device fabrication steps and demonstrating the first technologically relevant feasibility examples atypiqual will set the stage for a new quantum technology platform for atomically tunable multifunctional devices with applications in nextgeneration electronics photonics and spintronics
isolated seamounts as windows into upper mantle geochemical heterogeneity

hundreds of thousands to millions of seamounts unrelated to hotspot activity remain unexplored at the seafloor either as isolated seamounts or as clusters most of them are located at remote sites within the ocean basins and they are not influenced by deep mantle upwelling processes or subductionbackarc regimes thus their composition and mechanisms of formation are intrinsically related to the upper mantle compositional nature and dynamics for many years the upper mantle has been considered as a homogeneous and depleted reservoir nevertheless the evergrowing isotopic data available suggest that heterogeneities are pervasively present throughout the mantle the isosea project aims to characterize upper mantle heterogeneity using the composition of nonhotspot isolated seamounts and seamount provinces as a window into the upper mantle using an integrated approach including petrological geochemical and geochronological evidence from the seamount province located south of the midpacific mountains as the main case study we intend to characterize their mantle sources in an effort to understand what is the origin and composition of enriched upper mantle heterogeneities that allow the formation of seamounts unrelated to hotspot activity the results of this study could help lead to a paradigm shift of the composition of the upper mantle from considering it as an inherently homogeneous depleted reservoir to a possibly highly heterogeneous one containing enriched recycled lithospheric components the outcomes of the isosea project will have a strong impact on the european and international scientific community since they can be used to assess targets for future expeditions to unlock the secrets of the seafloor the final frontier on our planet
glass and metal mutual biocorrosion conservation of museum collections in storage

in the field of heritage conservation the longterm preservation of archaeological and historical objects held in deposits is a subject of increasing concern artifacts made from two or more different materials in contact with each other often stored under uncontrolled environmental conditions of temperature humidity and clearness can undergo degradation faster than thought the contact areas and interstices between two materials are especially critical points for joint degradation phenomena and prolific ground for microbiological contaminants especially in conditions of high humidity and in presence of organic pollutants with this in mind i am herein proposing an innovative project to investigate the degradation processes and microbiologicallyinduced corrosion of glassmetal objects that can occur in museum storage environments i will capitalize on my own experience in heritage science and glass alteration mechanisms and on the competences and skills of the host institutions in electrochemistry and microbiologically induced corrosion to i identify the major glassmetal joint corrosion phenomena and the microbiological contaminants present in museum storage deposits ii investigate the mechanisms underlying glassmetal corrosion and biocorrosion mechanisms by performing laboratory corrosion tests and iii experiment the application of electrochemical impedance spectroscopy eis to monitor the previously described corrosion mechanisms the proposed project will advance the understanding of joint biocorrosion processes occurring at the interface between glass and metallic materials and will hopefully establish a new methodology to monitor such mechanisms i will be extensively trained in corrosion processes and electrochemical techniques microbial processes and related laboratory procedures which will enable me to become an independent researcher and ultimately establish my own research group in the field of biodegradation and biocorrosion
selfpowering electronics the key to sustainable future

scientific and technological achievements are often accompanied with pollution of the planet even electronics believed clean produces waste counting in trillions of batteries we propose a solution that consists in batteryless selfsupplying and thus helping to keep our planet greensurprisingly not only seas are exhibiting tides the atmospheric pressure p and temperature t also do something similar sfinks will try to spark a new era of batteryless electronics via exploitation of the natural pt tides to produce electricity in innovative microgenerators the magic of the pt tides resides in that they are alwaysdelivering and omnipresent such generators will thus produce energy continuously everywhere regardless their location installation or orientation this sounds like a dream but5 years after i got this idea my phd students found a wonderful confirmation that the dream may become true early 17th century cdrebbel constructed an eltham perpetuum clock based on the principle of pt tides one of its followers the beverly clock is still running despite never having been wounded since 1864 however for all these 400 years the invention has remained relatively bulky and stagnated at mechanical energy stage in sfinks we will firstly miniaturize it towards monolithic silicon technology and develop innovative cointegrated conversion to electrical energyour target will be the first alwaysdelivering generator using an omnipresent energy source the first free of any installation constraints and also the first microscale generator using pt tides to produce electricity it will unblock further expansion of internet of things removing its bottleneck ie batteries thanks to their quasiperpetual lifetime the sfinks harvesters are also well suited to work on mars venus or on board of satellites where the p or t variations are very present our dream is to send a few sfinks powered sensors with a space exploration mission on mars
secure authentication of products for everyone

scantrust offers breakthrough anticounterfeiting technology and an accompanying software platform to grant brand owners and consumers high security and active traceability unlike regular qr codes scantrusts patented and proprietary secure qr codes named sqrcodes have intrinsic protection against counterfeit attempts the sqrcode can be placed on any product and allows anyone to authenticate a product and find out about its traceability this scalable innovation seamlessly integrates with standard widely used systems and devices used by businesses in production and distribution qr codes internal software systems the packaging industry standard digital printers and consumers smartphones in order to empower each user at any stage of a supply chain to instantaneously verify a product
verification and analysis for safety and security of applications in life

vassal project focuses on leveraging the scientific excellence and innovation capacity of the consortium in the field of software engineering to support safety and security of digitised economies and societies the key objective is to bring together expertise of the consortium in specific domains to enhance and create a new knowledge for robust and resilient sw engineering through scientific strategy combining modelbased design formal methods and economics for sourcecodes and systems as sw becomes more complex ensuring its safety and security vulnerabilities free becomes increasingly challenging and vital not only in safetycritical systems but across various operation systems and iot as well formal methods and modelbased design are taking an increasingly significant role building upon its already widespread use in safetycritical applications such as automotive or aerospace as basic building blocks in engineering process they enable to build robust and resilient sw and hw systems securitysafebydesign ensuring the reliability and correctness leveraging cybersecurity and improving the development lifecycle that lead to savings in operational costs of systems the economic assessment and implications of advanced sw engineering tools are not commonly available vassal will deliver a rare opportunity to explore and document the potential benefits of and challenges in deployment in order to leverage the awareness and make inroads to broader exploitation by endusers especially smes in segments where these issues are being overlooked due to the alleged nonreturnability of the investment
atmospheric flow loads and power for wind energy

the objective of the flow project is to develop new and innovative prediction methods for production statistics and load performance of modern gwscale and 400metre tall offshore and onshore wind energy systems our project will develop more accurate methods as regards the present state of the art sota and with high confidence thereby reducing uncertainties increasing productivity and grid stability lowering levelised cost of energy lcoe while establishing an opensource knowledge hub that will benefit the entire renewable energy sector and enable joint optimization between developers and oems to reach these ambitions flow will improve the knowledge of atmospheric flow physics and the interplay between wind farm microscale and largescale mesoscale processes such as wind farm global blockage farmfarm interaction and topographic and wakeadded turbulence in complex terrain based on these principles flow will develop and validate simulation tools that can be readily adopted by industry to lower economic uncertainties and enhance system reliability and power production with wide economic and societal impacts the proposed modelling framework will make extensive use of public experimental datasets to validate and train models within a fair data hub the new european wind atlas newa database is the foundation to grow this innovative opensource ecosystem that links experimental data flow models and validation datasets for benchmarking and training industry adoption will be facilitated through a computationally efficient modular framework that allows scalability in a production environment flows industrial partners comprised of vestas sgre ge and edf will verify compliance with operational processes and test the framework using private datasets to extend the validation range and demonstrate the added value of our results at a wide european scale
v4f

v4f aims to show proofofprinciple of a new technology capable of unprecedented control over interactions with specially synthesised targets to significantly improve the energy balance of aneutronic fusion reactionsnew concepts and advanced simulations of inertial confinement of aneutronic fusion reactions and particle acceleration will inform pioneering experiments in highenergy matterinteractions results could offer the prospect of breakthrough increases in alphaparticle yields from fusion reactions and mitigate the instabilities found in conventional fusion reactionsthis work offers the tantalising possibility of aneutronic fusion as a wastefree nuclear energy source and radical new configurations of particle accelerators leading to an efficient positron beam acceleration the results will benefit society with gamechanging new approaches to clean safe energy production and significant downscaling of positron accelerators with dramatic impacts in medicine industry and fundamental science
novel engineered cytokines for human therapy

cytokines are biomolecules of great potential interest for human therapy they modulate the immune system and play an important role in cancer inflammation immune response and tissue regeneration despite their great potential there are only a handful of cytokines approved for therapeutic purposes this is because many of them can have adverse side effects as they usually target different cell types which adds to their low serum halflife high production costs or lack of physiological efficacy different methods have been proposed to improve the pharmacodynamics and pharmacokinetics of selected cytokines however other properties do also need improvement such as achieving effective local concentration at the target site promoting the right activity in cytokines with dual functionality and decreasing toxicity by removing binding to unwanted cell types to help solving these issues we have developed a new protein design strategy that can be applied to all helixbundle cytokines many of which have been shown to be therapeutically relevant the resulting products are uniquely modified cytokines with improved properties amongst such properties our products show increase stability higher affinity and specificity for their target receptors and should have reduced toxicity all of which translate into greater safety and efficacy we have initial evidence both in vitro and in vivo of the superiority of our designs over conventional cytokines and in this project we will fully validate our technological platform and advance in the development of a business plan following the advice of several venture capital and pharma companies contacted to date with the idea of laying the basis for the creation of a new startup company
realease constraints of desalination

access to drinking water is one of the major issues of this century saltwater resources could constitute an almost infinite source of drinking water but their desalination remains expensive and technically challenging thus limiting its deploymentrealease aims to develop easily deployable costeffective and more sustainable desalination solutions to preserve water resourcesthis project relies on innovative technology developed at the cole normale suprieure ens paris and proposes to offer a novel and promising desalination solution unlike traditional desalination methods which require the application of extremely high pressures which can exceed 60 bars equivalent to the pressure under a 600m dam our approach exploits unconventional nanofluidic transport phenomena to purify water through commercial desalination filters using only low electric fields this allows desalination with less demanding less expensive and safer equipment for operators
the geopolitics of cloud computing how statefirm interactions shape the geography of computation to produce digital sovereignty and dependence

todays digital superpowers the united states and china exploit other countries reliance on their digital infrastructures for foreign policy and national security advantage governments in europe asia and elsewhere try to navigate this great power competition to retain digital sovereignty a pivotal moment in these technology geopolitics is the ongoing shift to cloud instead of data being stored and processed on users own devices and on servers situated at organizations own premises storage and processing are increasingly moving into hyperscale cloud data centres dotted around the world this concentration generates significant economies of scale decreases capital costs for firms and public sector organizations and improves energy efficiency however it also creates new systemic risks and since most of these data centres belong to us and chinese technology giants will dramatically deepen other states technological dependence on themunless smaller states succeed in shaping the shift to their advantage in the geocloud project we will for the first time map this changing geography of computation and examine how different states are attempting to shape it with policies focusing on the digital superpowers key battlegrounds of northern europe and eastsoutheast asia we will develop ways of measuring on a country level how exposed public sector and financial sector digital services are to cloud providers and how this exposure is evolving over time we will use statistical analyses and interviews with policy makers and cloud executives to assess how effective government policies have been at shaping this exposure and at influencing the ownership and locations of hyperscale data centres we will moreover theorize how government policies are interacting with large technology firms business strategies to generate new geopolitical dynamics that shape this geography of computationand with it our prospects of digital sovereignty and dependence
interaction between plant viruses and the ubiquitinproteasome system

the ubiquitinproteasome system ups is the primary eukaryotic pathway for selective protein degradation and plays a central role in various cellular processes including plant immunity and antiviral defense the ups is a doubleedged sword as it provides important services to viral infections but likewise is part of orchestrating a proper immune response both driving virus evolution towards ups manipulation and exploitation despite this exiting constellation the interplay between ups and plant viruses has remained poorly studied along increased globalization in agriculture and the ongoing climate change new viral diseases affecting various crops have emerged globally with negative socioeconomic impacts the virups project will be conducted at the swedish university of agricultural sciences slu under dr hafrns supervision aiming to address the knowledge gap in the upsvirus interplay in plants our preliminary data shows that plant viruses enhance proteasome activity and induce different changes to ups components and ubiquitination together conveying a detailed response of the ups to viruses the project will characterize plant ubiquitinome dynamics upon infection with three different viruses in comparative virology to identify virus common and specific responses with details on differentially ubiquitinated proteins dups and targeted cellular pathways a parallel approach will exploit one of our viruses camv capacity to activate the proteasome because this phenotype shows wide variation among arabidopsis natural accessions this offers an exceptional opportunity to address correlations between proteasome activation and various disease intensity parameters in addition to forming the basis for a genomewide association study gwas for identifying ups regulators ups regulators components and targets obtained through this diverse set of approaches will fuel the discovery of novel upsrelated mechanisms in disease and crop protection strategies