Dragon09/odtu_chatbot huggingface dataset:
dotsinnanowires by nearfield illumination novel singlephoton sources for hybrid quantum photonic circuits

quantum photonic integrated circuits are ideal platforms for quantum computation and communication a longstanding issue in their realization is the lack of a deterministic quantum light source compatible with silicon indeed planar selfassembled iiiv quantum dots qds which provide highperformance quantum light sources i e single photons can hardly be grown on si and also lack the positioning control necessary for efficient postgrowth iiivonsi hybrid integrationnanowires nws in contrast are rodshaped nanoscale semiconductors that can host intrinsically sitecontrolled iiiv qds and can even be grown on si however qds in nws have yet to reach the performances of planar qds an issue that could be overcome by integrating qds in nws in photonic cavities such a solution is at present out of reach because conventional creation of qds during nw growth hinders their successful integration with cavities the nanowhyr project will allow the integration of qds in nws with cavities by realizing an unprecedented postgrowth creation of qds inside nws the new method consists of the incorporation of hydrogen in iiiv nitride nws whose bandgap can be controllably modified by hydrogen subsequent hydrogen removal by scanning nearfield illumination will permit tuning the bandgap of the nw in a nanoscale region this will allow us to form a qd in that region with a widely tunable energy the deterministic control of the qd position and energy is the key that will enable us to efficiently integrate the qd with a planar photonic cavities fabricated on si and b vertical nw cavities grown on si this will lead to the breakthrough of a sitecontrolled electricallydriven telecomfriendly single photon source in si photonic circuitsmoreover this new strategy permits the modulation of semiconductor properties at the nanoscale and it is thus expected to open new grounds in other research areas such as photovoltaics and thermal energy converters
overnight vulnerable emotion reset normalizing invalidating generalized hyperaroused tension

anxiety and insomnia account for onethird of the global mental health burden prognosis is poor chronicity and relapse are common because treatment effectiveness is moderate while this situation calls for treatment innovation the required knowledge on targetable key mechanisms is lackingtheir shared core symptom is hyperarousal covering subjective physiological and clinical indicators hyperarousal is not just another overlapping symptom individual differences in hyperarousal determine severity prognosis and treatment response across insomnia anxiety and stressdisorders hyperarousal links their genetic and phenotypic overlapsince hyperarousal plays such a pivotal role in the development and chronicity of these disorders a transdiagnostic mechanistic understanding of hyperarousal would have major implications for the direly needed development of better treatment this project aims for a transdiagnostic mechanistic understanding of hyperarousal across wake and sleep and use to this insight to innovate treatmentthe project will do so by combining largescale datadriven approaches with targeted testing of the novel hypothesis that hyperarousal may be reset overnight crossing disciplines and diagnoses the project will first deliver an integrated account of the subjective brain structural brain functional and physiological hyperarousal landscape this approach will deliver unprecedented transdiagnostic insights into the lifetime development and underlying brain mechanisms of hyperarousal vulnerability and its consequences for mental health moreover this approach will yield both innovative stateofthe art tools as well as practical tools to quantify hyperarousal these tools will be employed in both experimental preclinical studies and immediately applicable transdiagnostic clinical interventions expected to considerably improve the perspectives of people suffering from the most common mental disorders by alleviating hyperarousal overnight
multidimensional approaches for comprehensive olive oil authentication

virgin olive oil is probably the most representative and iconic component of the mediterranean diet no industrial processes or chemicals are added during production thus providing an extra value reflected in its higher price compared to most edible oils for this reason the assessment of the quality safety and authenticity is of importance from both economic and health perspectives considerable effort has been put during the last decades in this direction organizations such as the european union the international olive council and the codex alimentarius faowho have established guidelines and regulations to protect the quality and authenticity of virgin olive oil and to prevent fraud however despite these efforts towards developing new procedures for olive oil quality and authenticity assessment appropriate solutions for some specific issues have not been found yet current gaps in eu analytical methods include the detection of selected blends of olive oil with other vegetable oils seed oils to identify fraud hence it is urgent to identify and improve analytical solutions that can detect both standard and emerging frauds and to meet all the international market required information macooil multidimensional approaches for comprehensive olive oil authentication aims to propose novel cuttingedge solutions based on mass spectrometry previously unexplored in the field to provide adequate solutions for some of the challenges in terms of authenticity that need to be addressed in the virgin olive oil sector this project will develop novel tools to extend the potential to detect fraudulent practices of mixing virgin with refined deodorized lowerquality olive oil
deciphering the genetic basis of chronic kidney disease towards prevention and personalized therapy

chronic kidney disease ckd is a public health challenge affecting millions globally patients have increased risks of mortality morbidity and progression to endstage kidney disease eskd ckd is often clinically silent disease mechanisms are unclear and targeted therapies are unavailable recent studies support considerable contributions of underappreciated monogenic aetiologies in adults with ckd suggesting that most inherited kidney diseases remain undiagnosed untreated and poorly understood our overarching objective is to unravel the genetic basis of ckd towards early diagnosis and genebased therapy we therefore established the israeli eskd genetic cohort and already recruited clinical data and samples from 2000 participants with eskd our main objectives are 1 to unravel known and novel ckd genes by interrogating highrisk groups for genetic ckd using next generation sequencing on a population level 2 to study novel monogenic ckd disease mechanisms by using patientderived kidney organoids and mutationspecific mouse models specifically we will study a newly identified wdr19 mutation leading to many adult onseteskd cases in the israeli arabdruze population and 3 to develop genebased targeted therapies for ckd we will focus initially on monogenic ckd secondary to gainoftoxicfunction mutations in umod encoding uromodulin a kidneyspecific protein this relatively frequent adult ckd aetiology accounts for 052 of ckd cases worldwide altered uromodulins toxic effect is suitable for novel rnabased therapies gene silencing of uromodulin with antisense oligonucleotides will be performed and studied using a patientderived in vitro model as well as a umod mouse model this project will improve ckd classification and clinical management reveal disease mechanisms as well as novel treatments in a broader perspective this project will set the stage for future clinical trials and for other rnabased genespecific ckd treatments
microbial hydroponics circular sustainable electrobiosynthesis

for the first time mihy brings together microbial fuel cell mfc technology and hydroponics thereby introducing a prosthetic rhizosphere an extended rhizosphere community to the typically soilless configuration of hydroponics systems the mihy system will modulate nitrogen forms plant root microbiomes optimise nitrogen uptake and mobilise phosphorous averting the need for chemical fertilisers this circular sustainable platform turns carbon into biomass and reclaims nitrogen from wastewater streams linked through shared microbiomes the bioelectricity generating bioelectrochemical system bes microbial fuel cell mfc platform is i optimised to generate electricity from wastewater at 1mw1ml feedstock setting a new benchmark for mfcs ii driving wavelengthspecific leds to optimize photosynthesis iii capable of recovering useful biomolecules using adjacent mfcs to modulate the redox potential of a workhorse microbial electrolysis cell mec performing microbial electro synthesis mes this novel development brings together mfcs mecs since these share common microbial constituents they can be technologically combined without the need for external fossilfuel based energy sources to achieve this goal mihy aims to design biofilms using metabolic engineering in wild type symbiotic strains this nextgeneration hydroponics system has nearfuture applications in agriculture and in the urban environment it delivers a smart decentralized low power digital infrastructure with sustainable organic solutions which are in line with the ambitions of the missions cities climate adaptation urban agriculture precision gardening wastewater treatments energy generation synthesis of high value compounds eg vitamins mihy will also invite designers and stakeholders from various sectors to cocreate future applications ultimately it anticipates and facilitates a healthier sustainable naturebased urban landscape
mapping out sharks alternative to bone the innovation of calcified cartilage

sharks and their relatives are distinctly unique compared to other vertebrates including humans they have an internal skeleton made entirely out of cartilage it was presumed that cartilage was the ancestral condition among vertebrates based on the assumption that evolution would be driven toward the increased complexity of bone however recent fossil evidence disproves this assumption and reveals that the skeletal mineralisation shown in sharks calcified cartilage is an evolutionary innovation our understanding of calcified cartilage in sharks greatly lags behind that of the hard tissues in other vertebrates thus the mechanisms behind cartilage calcification how or whether this relates to bone and our overall understanding of how hard tissues evolved in vertebrates is impacted in this project i will address this knowledge gap by examining the 3d histology of calcified cartilage in a wide range of both living and fossil sharks using cuttingedge scanning methods this holistic approach will cover 400 million years of cartilage evolution in sharks the results of this project will be of importance across multiple scientific fields advancing our understanding of the evolution of hard tissues in vertebrates detailed 3d information on structural properties of calcified cartilage relevant to material engineering and insight into calcification mechanisms relevant to medical research on cartilage disorders in humans naturalis is the perfect setting to conduct this research due to the vast collection of preserved sharks available for study expertise in hard tissue histology and active collaborations with medical researchers on cartilage disorders it also offers a valuable platform for public outreach and skills training such as in management and science communication this project will provide a crucial stepping stone towards my aspiration to spearhead a research team focused on bridging research of both fossil and living animals
adaptationoriented seamless predictions of european climate

aspect aims for the setup and demonstration of a seamless climate information sci system with a time horizon up to 30yr accompanied by underpinning research and utilisation of climate information for sectoral applications middleground level1 the goal is to improve existing climate prediction systems and merge their outputs across timescales together with climate projections to unify a sci as a standard for sectoral decisionmaking the focus will be on european climate information but we will also look more widely where there is a policy interest eg disaster preparedness and in regions of european interest we will maintain a strong link into an exploit learning from the wcrp lighthouse activities on explaining and predicting earth system change to provide a bandwidth diversity of information the sci system will be based on multimodel climate forecasts and will build on learning from projects such as eucp it will align with new activities on digital twins within europe including destine the sci will combine physical science aspects with those from other disciplines to ensure the information is robust reliable and relevant for a range of user driven decision cases the information package will incorporate baseline forecasts and projections plus uncertainty but also new frontiers will be explored eg extremes which are of socioeconomic highlevel interest to be successful the research will encompass understanding and attribution of various processes along the timescales such as exploring signaltonoise ratio and their impact on predictability new ways of initialisation of the prediction systems merging predictions with projections provision of regional sci for europe by downscaling statistical methods ai and highres models including convectionpermitting models and innovative postprocessing method enhancing the skill and robustness of the climate forecasts
fully automated cellfree dna extraction and quantification liquid biopsies safely from patient to lab

liquid biopsies are blood tests with growing potential in diagnostics for cancer and prenatal screening preanalytical challenges such as the need for skilled workforce and strict logistic requirements for samples transportation are limiting the full exploitation of the method to address these technical issues we developed a method for immediate and automated sample processing that provides a sample with higher reliability less human labour and less transport requirements biopsense innovation is a fully automated disposable cartridge where the blood is filtrated using a dedicated hollow fiber membrane and then cfdna is isolated from the plasma in the microfluidic system the resulting sample can be then transported by regular post and eluted at the destination to carry out the final analysis the cartridge is controlled by a control unit composed of a vacuum generator software and a simple spectrophotometer in this eic transition project our objectives are to validate the automated cfdna extraction technology and to integrate all the features of the innovation in a single disposable cartridge which will be ready for marketing licensing and ivd validation by the end of the project the cartridge design will be done in close collaboration with the potential customers sector biotech companies and hospital laboratories to use their feedback to develop the user interface manufacturability and regulatory compliance the project will focus on the cartridge design and will outsource control unit development to a company with experience in vitro diagnostic devices designing and production in this project the commercialisation strategy for the next 510 years will be developed to define how our startup company biopsense will handle the disposable cartridge design and commercialization and further rd of the technology in additional applications
elucidating drivers of human inflammatory bowel disease by nextgeneration organoid modeling

inflammatory bowel disease ibd is a collective term for bowel diseases caused by undesired immune reactions and can currently not be cured to develop adequate treatments for ibd we need to understand the nature and causes of the pathogenic immune responses observedto identify mechanisms driving ibd in humans we need apart from diagnostic correlative studies adequate in vitro cell models that realistically recapitulate the in vivo situation and allow for intervention studies thus far in vitro models that incorporate next to intestinal epithelium also supportive stroma and immune cells are lacking in my phd studies i generated a map of the ibdassociated immune cell network by singlecell analyses of healthy and ibd patient samples in my postdoctoral work i have developed an air liquid interphase ali intestinal organoid platform derived from human samples that supports tissueresident immune cellsin this project i aim to identify cellular and molecular networks driving ibd by diagnostic and intervention studies in intestinal ali organoids for this purpose i will 1 validate ali intestinal organoids as accurate ex vivo model by analyzing their immune cell composition and functionality in spatial context and 2 aligning this with the in vivo situation by data mining of public singlecell rnaseq of inflamed intestines 3 integrate datasets generated in 12 to reveal cellular and molecular immune cell interactions associated with ibd and 4 implicate interactions identified under 3 in driving ibd by molecularly targeted interventions in ali organoids the proposed work will take place at host institute leiden univ medical center with a 4month secondment phase at technical univ delftthis project is expected to deliver insight into immunological mechanisms driving ibd and clinically translatable therapeutic interventions by mechanistic studies in a novel in vitro system mimicking the in vivo cellular environment of human ibds
into the sedimentary matrix mapping the replacement of neanderthals by early modern humans using microcontextualized biomolecules

the demise of neandertals and the peopling of europe by anatomically modern humans amh occurred geologically speaking in the blink of an eye while we know that neandertals and amh interbred how and where this interaction occurred remains unresolved now that humans can be fingerprinted from the adna they left behind in sediments highresolution site formation studies are essential to establish microstratigraphic integrity and to reconstruct the human past at finer scales matrix will zoom into the sedimentary matrix at an unprecedented resolution using adna but also proteins and lipids stored in archaeological sediments in order to 1 find the identity of past hominins in wellpreserved microcontexts 2 reconstruct both their behaviors diet use of fire and environments they lived in and 3 contribute to rewriting what happened at the time of neandertal disappearance by integrating highresolution molecular and microscopic records we will focus on seven selected sites distributed throughout europe and with established adna preservation we will assess the microstratigraphy of deposits from 50 to 40000 years bp by studying intact archaeological samples in 2d and 3d micromorphological views we will obtain adna data at a microscale and couple it with bone proteins and lipids from organicrich sediment samples all extracted from intact micromorphological samples the project aims to achieve for the first time an integration of biological behavioral and environmental information of archaeological deposits at a mm and submm stratigraphic scalepioneering the application of microarchaeological techniques linked with contextualized molecular data while setting a rigorous basis for their interpretation matrix will greatly improve our understanding of the migration of amh into eurasia and eventually to our inhabiting of the entire world
innovative imaging insights i3 development of mrcompatible bioreactors for tissue engineered heart valves

given the escalating global burden of valvular heart disease and the pressing need for living heart valve replacements i decided to focus my research on this healthcare challenge by acquiring new skills through advanced training and international interdisciplinary collaborations my proposal i3 combines the design and application of a novel bioreactor for assessing tissue engineered heart valves tehvs by highly advanced magnetic resonance imaging mri to kickstart the innovation cycle in invitro experiments especially young patients with valvular heart disease will profit from tehvs as they suffer from current heart valve replacements lacking the ability to selfrepair and growth as well requiring the lifelong intake of anticoagulantsleveraging the highdimensional noninvasive analysis potential of mri my i3 proposal establishes new innovative tools for tehv research to enable new insights in the resulting hemodynamics downstream of tehvs close the optimization loop invitro using patientspecific boundary conditions and reduce the need for ethically and monetary challenging animal experimentsbuilding on my expertise on advanced mri i will acquire new skills to develop a novel mricompatible bioreactor in the project enabling invitro experiments with high precision and repeatability the imaged flow field in the bioreactor allows me to select and optimize a tehv for specific hemodynamics of patients invivo mrienabled feedback of the resulting hemodynamics offers further diagnosis furthermore invitro and invivo measurements offer important information for optimization of tehvs for patientspecific treatmentsi will use the knowledge and skills gained from this intersectoral and interdisciplinary project for my next career steps incl submission of an erc starting grant in the field of biomedical engineering research aimed at providing all patients with a suitable heart valve replacement i3 is a major step towards making this a reality
innovative chemistry to illuminate biology a new chemical conjugation technology to optimize drug discovery

drug discovery faces two key challenges on one hand the sector has been losing competitiveness due to an increase in development costs and a reduction in sales and on the other hand the new therapeutical targets that appear every year in part thanks to the potential of personalized medicine demand urgently new research tools to efficiently identify new drugs acting on them celtarys proprietary technology provides a solution to both challenges it is a new and disruptive patented chemical conjugation methodology that allows a qualitative and quantitative leap in the development of fluorescent chemical tools required to optimize early phases of the drug discovery process thus reducing the attrition rate in later clinical phases and therefore optimizing the efficiency of the process at the same time our technology makes possible to develop in a costly and timely competitive manner customized fluorescent tools for any target of interest unlocking the potential of newly identified therapeutical targets our technology is an efficient and environmentally friendly legolike semicombinatorial conjugation strategy accelerating the design and synthesis of fluorescent probes with optimal pharmacological and photophysical properties for any target with therapeutic interest outperforming radioligands and other available fluorescent probes thus improving the complex and costly drug discovery process celtarys is led by two women both founders of the company maria majellaro phd in chemistry inventor of the patent in which our technology is based and cso and sonia martnez arca phd in biology with previous experience in entrepreneurship and ceo of the company celtarys has been founded in 2021 and has secured a seed investment round we count already with products in the market and our first clients our priority for the short term is consolidate our market position and undertake a growth phase for which we seek support from the women techeu program