Dragon09/odtu_chatbot huggingface dataset:
moving from whole genome cfdna methylation to a pcrbased liquid biopsy assay for detecting highrisk atherosclerotic plaques

general practitioners and cardiologists are faced every day with challenging decisions on whom to refer for diagnostics of coronary artery disease cad as sign and symptoms of cardiac ischemia require immediate action diagnosis of cad is key as ischemic heart disease accounts for 45 of deaths in females and 39 in males in the european union yet diagnosis of cad in women is regularly delayed and often unrecognized as the main pathology underlying cad it is essential to understand atherosclerotic plaque biology in recent years my research group and others have identified clear sex differences in the atherosclerotic plaque morphology and its epigenetic regulation one consequence is that female plaques are more difficult to detect than male plaques especially those which cause symptoms as part of the erc ucare project we uncovered a plaquespecific epigenetic signal in cellfree dna from plasma of women with cad this signal consisted of hypomethylated regions specific for plaques that can be detected in plasma cellfree dna our methods thus far have been focused on whole genome sequencing analyses yet for studying the predictive value of these regions in large cohorts of patients with cad as well as exploring commercial potential we need to develop a more specific methodology our aim in this current ubiomarcare project is to investigate if the methylation status of plaquespecific regions can be detected in plasma with a pcrbased method once we prove that this methodology is reproducible and comparable to whole genome dna methylation sequencing we can perform large biomarker studies in both women and men suspected of heart disease and explore potential commercialization of plaquespecific cellfree dna methylation profiles to accurately detect cad this will be a game changer for the healthcare sector as it will allow a specific and timely detection of dangerous atherosclerotic plaques both in men and women
sustainable biobased and bioinspired materials for smart technical textiles

in subbimatt we will develop a novel generation of biobased and bioinspired sustainable smart technical textile materials to address current and future energy needs we start from developing debondable adhesives biopolyurethane and negative thermoresponsive materials complementing these with existing but tuned biobased materials will enable circular textile intermediate products high end coated fabrics nanomembranes and shape memory filaments subsequently these textile intermediates will be the stepping stone for making bioinspired smart textile materials stm featuring mechanical actuation energy harvesting and tuneable fabric openness lastly the novel biostms will be implemented in three demonstrators with integrated energy harvesting shape morphing building envelopes automotive interior textiles and advanced garments with adaptable breathabilitythe safe and sustainable by design ssbd framework will be applied developing a protocol for technical textiles development combining modelling and experimental characterisation will allow to optimise the development of the stms and demonstrators predicting their safety functionality and energy harvesting capabilities for the demonstrators we plan to integrate complimentary elements eg sensors batteries or supercapacitors resulting from other projects via liaison subbimatt results will help to find more sustainable ways for covering the most important consumer living standard needs energy sustainability and comfort the demonstrators will be products that citizens use on a daily basis thus helping to increase visibility and impact that way we aim to create a positive momentum for the novel generation of smart biomaterials and for their application in bioinspired technical enduses thus helping the eu technical textile industry to remain competitive and become more resilient while transiting to a climateneutral and circular economy
leveraging sacrifice to strengthen relationships the importance of couples sacrifice communication

romantic relationships are essential for health and wellbeing in fact a poorquality relationship is a more powerful predictor of increased mortality than smoking yet divorce rates have more than doubled among the 27 eu countries in the last five decades one reason why relationships are hard to maintain is because couples face difficulties navigating challenging interactions such as when one partner sacrifices their preferences for the other sacrifice occurs regularly and can fuel resentment thus putting relationships at risk yet sacrifice also may convey that the sacrificer cares about the recipient and thus has the potential to strengthen relationships given the profound impact of relationships on health and wellbeing it is crucial to identify how couples can reduce the costs and reap the benefits of sacrifice to maintain satisfying relationshipsexisting research examining the impact of sacrifice has focused on individuals internal feelings and motivations prior work however overlooks how individuals express their feelings and thoughts about the sacrifice and neglects the dyadic interaction between the sacrificer and recipient saycrifice aims to address these critical gaps by examining 1 how both partners communicate about sacrifice and 2 how sacrifice communication styles shape personal and relationship outcomes saycrifice will integrate across different fields relationship science communication social cognition and combine stateoftheart methods dyadic observational diary and longitudinal methods implicit and explicit measures to provide the first comprehensive investigation of couples sacrifice communication in close relationships the findings will reveal effective ways for couples to communicate about sacrifice which can be used to develop interventions to promote satisfying relationships transforming sacrifice into a catalyst for building happier relationships will in turn improve societal health and wellbeing
theater in imperial spain studying a commercial activity during the first globalization from the perspective of labor and economic history

the overall objective of this project is to study the hispanic theater professionalization process during the second half of the 16th century and the early decades of the 17th century from the perspective of labor and economic history furthermore i approach this process in light of the first globalization that is considering both spain and its overseas colonies as well as the interconnections between them this is imperative because golden age professional theater has been and continues to be subject of many studies especially philological but we have not fully understood the basis of the functioning of this new profession that emerged in the midsixteenth century the reason is clear we are dealing with a commercial activity that has not been specifically and systematically studied as such since to do so it is necessary to understand its economic and labor dynamics in addition professional theater has not been studied as a global phenomenon and this is also a serious problem because in the sixteenth century imperial spain expanded into america and this fact cannot be ignored much less if we are talking about a profession that developed almost at the same time on both sides of the ocean the project has four phases in the first one a database will be created to collect and organize theatrical economic information the database is intended to become an openaccess tool during the other three phases of the project labor and economic history methodologies will be implemented to reconstruct cases of theatrical enterprises to analyze the labor and economic conditions of different types of comedians and finally to calculate approximately the costs of staging a play at the end of the sixteenth century thus this project would introduce new methodologies to theatrical studies and would inaugurate a brand new line of research founded on the economic history of an activity that is art literature and also business
revaluing urban quality climate neutrality in european waterfront cities

in revalue 9 european waterfront cities lesund bruges burgas in revalue 9 european waterfront cities lesund bruges burgas rimini cascais constanta izmir psek and rijeka will test capture and share how to create value through urban quality in a holistic approach towards climate neutrality they will cover 6 systemic challenges of urban planning and design aligned with the cities mission systemic changes in governance regulatory structures advocacy societal and spatial quality financial and circular value chains datadriven cocreation digital twins energy and mobility naturebased solutionsthe model will be supported by 3 innovation cycles that run across the cities aligning climate neutrality and urban quality using participatory storybuilding to identify cobenefits cocreating datadriven transition scenarios empowering cities to use better data data better derisking investments through valuebased financial and partnership modelsto enable the cities to document and capture different values in urban complexity and to synthesise them into a holistic approach towards climate neutrality supported by this approach the 9 revalue cities will finetune share and test a portfolio of urban design and planning methods in the projects community of practice 4 leading cities lesund bruges burgas and rimini will demonstrate fullscale how integrated urban planning and design can be optimally deployed to achieve climate neutrality and significantly reduce ghg emissions by 2030 5 replication cities cascais constanta izmir psek and rijeka will learn replicate and develop their own visions scenarios and investment plans to accelerate their journeys to climate neutrality while geographically and culturally diverse the 9 cities share a curiosity and ambition to experiment learn from each other and share the revalue results and outcomes together with other cities in the cities mission communities
3dimensional organization and functions of translation in organelle proximity

in eukaryotic cells many proteins are produced next to the organelles where they function demonstrating a link between translation and protein targeting the mechanisms of this connection are mostly unknown in this proposal i bring together the fields of translation regulation and organelle biogenesis to find this out i approach the problem with a powerful combination of functional genomics and cryogenic electron tomography cryoet first i want to create a quantitative map of proximal translation next to all the organelles and their subdomains using highthroughput imaging i will determine localizations of all the mrnas and their dependence on translation i will also map the positions and orientations of ribosomes next to all the membranes using proximity labelling and direct quantification of native ribosomes with cryoet beyond mapping i want to understand how this local translation map is adapted to different conditions using highthroughput screening i will determine what global and local factors establish and regulate proximal translation at each organelle finally i will investigate the role proximal translation plays in essential organelle functions and get mechanistic insights into how local regulators help to carry it out when completed the project will overturn the binary view of either sec61coupled translation on the er surface vs free cytosolic translation of all the other organellar proteins instead i will uncover a holistic picture of multiple proximal translation locales with distinct regulation i aim to discover new connections between the two fundamental processes of protein synthesis and organelle biogenesis and thus make a groundbreaking advance in the understanding of how cells coordinate their architecture and functions
fundamental fields and compact objects new opportunities

the celebrated detection of gravitational waves gws the first observation of horizon scale structure of a black hole bh together with the developments in the field of numerical relativity are allowing access to extreme strong and dynamical gravity configurations both observationally and theoretically making our time a golden epoch for studying extreme gravitational systemsin parallel families of exotic but theoretically sound models of bhs and horizonless compact objects in the presence of new fundamental fields or alternative theories of gravity have been constructed to a large extent numerically these can be submitted to computational experiments to test their dynamics and compared against observations to address the issue of degeneracy in the theoretical interpretations of strong gravity data or even detect putative better fits to the data at the meeting point between these two developments lies the promise of new fundamental physics a deeper understanding of bhs and neutron stars unveling the nature of dark matter and dark energy delivering on this promise requires a synergetic endeavour that must bring together different groups and competences this project creates a multiconnected team with different and complementary scientific expertises on bhs neutron stars and exotic compact objects their phenomenology gws lensing and astrophysical environments and the techniques necessary to extract the latter from the former for comparison with observations including constructing and evolving solutions of nonstandard compact objects with numerical methods building gw libraries lensing images and performing databayesian analysis and parameter estimation the team includes members of the ligovirgokagra and event horizon telescope collaborations providing a direct connection to observations and will explore deep learning frameworks to face the key challanges of model classification and data augmentation
european event on industrial technologies indtech 2022

the european industry is currently facing growing number of challenges and the status quo is not acceptable as a sustainable solution the two main trends of green and digital transformation is a strategic call for action for all individual companies but more and more also between them the trends of shortening of value chains not only from technological and datause point of view but also from deinternationalization perspective became very urgent as recovery measures after the covid19 pandemics and will be considered as a potential driver for future resilience building strategies at the same time the existing workforce will need to accept and adopt the proposed changes as a new opportunity for sustainable growth which shall be supported by development of new skills and largely available reskilling these and other aspects will be promoted and opinions from industrial leaders collected at the next paneuropean conference on industrial technologies in grenoble 2022 france the feedback will be provided for further policy work on european national and regional level
opening new frontiers in multiscale evolution of collider events a dual pathway to precision

for the decades to come the pioneering investigation of the fundamental laws of nature at the large hadron collider lhc and at future collider experiments will rely on the interpretation of complex scattering events by means of accurate theoretical calculations which are instrumental to find small signals of elusive new phenomena these calculations must describe the evolution of the system from the few particles produced in the highenergy scattering to the tens or hundreds of lowenergy particles observed in the detectors this proposal aims at transforming the core of our theoretical understanding of such a multiscale evolution which nowadays is often too inaccurate to cope with the striking experimental precision the goal of janus is to develop innovative ideas and theoretical methods to advance significantly both approaches to the problem resummations and partonshowermontecarlo psmc generators janus will establish a deep connection between the two fields resulting in novel technology for the accurate modelling of multiscale effects its main objectives are to formulate new theoretical methods to tackle the resummation of complex collider observables in reactions with more than two emitters and scales with stateoftheart accuracy to lay the theoretical foundations for a new generation of psmc algorithms capable of describing accurately the hard scattering and subsequent partonshower evolution for reactions with different jet multiplicities with a new standard of precisionthis technology will be applied to obtain stateoftheart theoretical predictions for a broad range of critical observables in the important fields of higgs and jet physics enabling their optimal exploration at the lhc and future collider experiments
somatic mutations in vascularwall function and ageassociated disease

already in 1973 benditt and benditt found that the atherosclerotic plaque was clonal and proposed that it was a neoplasm of vascular smooth muscle cells vsmc recent technical advances have made it possible to analyze somatic mutations in human tissues and show that somatic cells accumulate thousands of mutations during development and aging while most of the mutations are harmless some either contribute to disease and aging or are directly diseasecausative as in the formation of tumors in cancer the arterial wall is in part composed of clonal vsmc elements making this tissue particularly vulnerable to somatic mutagenesis however detailed analysis and insight into the extent of somatic variance in the vascular tissue is lacking here we plan to analyze the somatic mutations in the arterial wall and relate it to ageassociated cardiovascular disease cvd we will create an atlas of somatic mutations in the human arterial wall at singlecell resolution in health and disease we will apply singlecell sequencing in arterial tissue to map somatic mutations functional significance of somatic mutagenesis in the vascular wall will be validated using data on gene regulatory networks of relevance in human cvd and disease models with induced somatic mutations in vsmc the model will have a mosaic pattern of mutation and fate of mutated cells will be mapped by lineage tracing in the development and progression of cvd our research provides insight into the prevalence nature and functional significance of somatic genetic events in the healthy and diseased vascular wall
ultimo advancing sustainable usercentric mobility with automated vehicles

during the past few years many projects and initiatives were undertaken deploying and testing automated vehicles avs for public transportation and logistics however in spite of their ambition all of these projects stayed on the level of elaborated experimentation and never reached the level of a largescale commercial deployment of transport services the reasons for this are many the most important being the lack of economically viable and commercially realistic models the lack of scalability of the business and operating models and the lack of user oriented services required for large enduser adoption of the solutions the ultimo project will create the very first economically feasible and sustainable integration of avs for maas public transportation and laas urban goods transportation ultimo aims to deploy in three sites in europe 15 or more multivendor sae l4 avs per site a user centric holistic approach applied throughout the project will ensure that all elements in a crosssector business environment are incorporated to deliver largescale ondemand doortodoor wellaccepted shared seamlessintegrated and economically viable ccam services we target the operation without safety driver onboard in a fully automated and mission management mode with the support of innovative user centric passenger services ultimos innovative transportation models are designed for a longterm sustainable impact on automated transportation in europe around the globe and on society the composition of the consortium ensures the interoperability between multiple stakeholders by making adoption of new technology at minimum costs and maximum safety the integration of the ongoing experiments of previous avdemonstrator projects ensures highest possible technical and societal impacts from the very beginning of the project as well as during the project lifetime and even long after its completion
instrumented human stem cells to see more during high throughput screening in druginstrumented human stem cells to see more during high throughput screening in drug discovery and regenerative medicine

in hipscmore read hipseemore my team and i will engineer fluorescent sensors in the dna of human induced pluripotent stem cells hipsc so we can see more during highthroughput imaging screenings imhts for drug discovery and regenerative medicinesince hipscs can differentiate in most cell types in the human body they are the ideal cell source for more predictively screening therapies yet hipscs are expensive to maintain and require external reagents to visualize pathophysiological changes which adds to the screening cost and complexity in fact the major hurdle in adopting hipscs for imhts has been the price increase from 010 data point to 2 data pointin the context of our erc stg we genetically encoded fluorescent sensors for cell structure function and cell cycle progression directly into hipscs for invitro modeling of heart development with those sensors we can visualize pathophysiological changes through reagents provided by the cells themselves thus the more experiments we conduct the cheaper they become we believe this is the strategy to bring hipscs into imhts where testing millions of compounds vanishes an experiments marginal cost like software to explore this opportunity we envision three main objectives1 technology feasibility we will scale up the production of our seemore hipscs and validate their costeffectiveness in drug screening and regenerative medicine applications2 business feasibility we will analyze market dynamics and customer preferences in the drug screening and regenerative markets to define the most efficient gotomarket strategy 3 executive management we will work with stakeholders in our advisory board to delineate ipr business strategy and future fundraising opportunities for a seemore spinoff companythrough these activities the seemore spinoff will incorporate the right mix of tech development and business savvy in its own dna so it can seize more of the hts market