Dragon09/odtu_chatbot huggingface dataset:
from co2 and nitrogen fixation to the delivery of therapeutic enzymes silicified dna origami as artificial microcompartments

all life is dependent on enzymatic reactions and functional enzyme cascades have been evolutionary optimized yet introducing foreign enzymes into other organisms through genetic engineering often results in reduced or no enzymatic activity in nature enzymatic activity and efficiency are often enhanced by encapsulation of enzymes in microcompartments resulting in protection and rate enhancement through high local concentrations of enzymes cofactors and substrates in nanocat i will engineer artificial microcompartments hosting highly efficient enzyme cascades that are operational in organisms where they do not naturally occur the approach is based on my recently developed method to controllably template silica nanostructures by dna origami these inorganic materials retain the attractive features of dna origami like precise localization and number placement of proteins of interest combining the power of dna nanotechnology with the sturdiness and biocompatibility of silica as artificial enzymehosting microcompartments i now have a highly disruptive tool at hand that has the power to outperform standard biological methods and be a true game changer nanocat is a novel approach to assemble complex enzymes outside of their natural environments to address health issues global carbon emissions and plant fertilizers something that current biological methods struggle to do the immense demand in todays agricultural climate and health needs urgently calls for engineered systems with full control over size shape and functionalizability to enhance enzymatic activity and endow organisms with new and improved properties nanocat aims to provide a solution to these issues through the assembly of artificial enzymehosting microcompartments from silicified dna origami with universal applicability which i will exemplify through the formation of carboxy nitrogy and galactysomes for co2nitrogen fixation and the cellular delivery of essential lysosomal enzymes
lucero bio smart optofluidic isolation of spheroids for earlystage drug discovery

personalised medicine has become a key focus for the pharmaceutical industry as it can address global health challenges such asstrong variations of drug efficacy and toxicity linked to different patient subgroups a major obstacle that pharmaceutical companies still face is the lack of enabling technologies to predict the effects of drug candidates in patients before entering clinical trials in preclinical drug screening procedures pharmaceutical companies demand that the technologies be selective in terms of detection and isolation of patientderived 3d cell cultures while being scalable for highthroughput drug screening lucero fills this need by providing a novel cellhandling platform that combines microfluidics optical tweezers and artificial intelligence during our erc poc project we developed an integrated benchtop device for invitro handling of single 3d cell cultures in particular mini spheroids lucero will build on this achievement by expanding our prototypes functionalities and adapting its design to automatically detect spheroid viability and fully automate their isolation to match the drug screening workflow by thoroughly testing the prototypes performance and compatibility with our large european pharmaceutical partner onsite we will mature our prototype to technological readiness level trl 6 based on their feedback we will increase our commercial readiness level crl 6 and will be ready for the next acceleration phasethe intended result of this proposal is to introduce luceros 3d cellhandling platform as an enabling tool for pharmaceuticalcompanies to develop more effective nearpatient treatments at a lower cost as well as to establish europe as a leader in precision healthcare
cryptography for second layer blockchain protocols

blockchain technologies offer an exciting new alternative for centralized platform providers they distribute trust over a large number of parties thereby promising major benefits in terms of security freedom and efficiency unfortunately due to their inherent distributed nature state of the art blockchains suffer from a multitude of shortcomings in contrast to centralized platforms they are restricted in terms of scalability confidentiality and functionality and thus cannot power large scale applications the ambitious goal of cryptolayer is to overcome this situation by building decentralized systems that can compete with centralized platforms we will achieve this goal via building offchain technologies that form a secure second protocol layer on top of the underlying blockchain the first component of cryptolayer builds a family of secure protocols for offchain transaction systems these protocols move the massive amount of transactions to a second layer thereby offering nearly unlimited scalability and instantaneous transaction processing our solutions will support smart contracts complex programs that can handle money and offer strong privacy guarantees by relying on tools from cryptography the second component of cryptolayer leverages advanced cryptographic building blocks to construct secure offchain services for confidentiality and access to realworld data this will enable rich classes of applications to run over decentralized infrastructures finally since secure protocol design is highly involved and errorprone we will build upon the formal framework of theoretical cryptography we will develop formal security models using the universal composability framework and carry out a rigorous security analysis using mathematical proofs with our overarching treatment of offchain technologies our vision is to establish this field as a central part of the rapidly evolving research on blockchain technologies
generation of tumor neoantigens with photodynamic therapy a new strategy for anticancer vaccines to fight head and neck cancer

immunogenic cell death icd is a regulated cell death that stimulates the immune responses resulting in the establishment of long term immunological memory icd alert the immune system by tumorassociated antigens and tumor neoantigens ie antigenicity and releasing immunostimulant mediators such as the damage associated molecular patterns damps adjuvanticity icd can be efficiently induced by photodynamic therapy pdt our key hypothesis is that pdt based on clinically approved and inhouse developed photosensitizers which are efficient in several murine cancer models will induce icd in head and neck squamous cell carcinomas hnsccs which are the fourth most frequent types of cancer in men specifically we aim to demonstrate for the first time that pdt will modulate the immunogenicity of dying hnscc cells not only by releasing damps but also by increasing antigenicity of dying hnscc cells by affecting their landscape of antigens this will lead to the activation of the innate and adaptive immune system and to the improvement of anticancer therapy of hnscc patients for the study we will exploit hnscc cells derived from patients biopsies representing an ideal preclinical model that incorporates heterogeneity of tumors observed in patients in this way we will obtain the data required to support future clinical trials based on pdt indeed the ultimate goal of the neoantigen identification is to pave the way for developing patientspecific targeted vaccine therapies
hydrogel biodegradable ureteral stent

hydrumedical sa hyd team is composed by a urologist and biomaterials scientists that will revolutionise the treatment of ureteral disorders hydrustent is a biodegradable hydrogelbased soft lubricated and flexible stent these properties prevent bacterial adhesion 80 of the stentrelated complications and encrustation development nowadays the application of stents requires a 2nd removal procedure a burden for patients and healthcare systems hydrustent is homogeneously degraded by the urine flow within 710 days eliminating the need of further interventions for its removal hydrustent will bring huge health benefits for patients and reduce up to 60 of treatment costs 43b savings for the health system in euus validated by the urology community with a global ureteral stents market raising 604m by 2030 if hydrustent project is successfully implemented we intend to reach the eu market in 2024 with profits amounting to 25m 5 years after product launch
methane paradox revisited unravelling the impacts of eutrophication on microbial methane cycling in aquatic ecosystems

aquatic ecosystems are a major source of the potent greenhouse gas methane accounting for half of the global methane emissions biogenic methane is microbially produced in anoxic sediments and typically rapidly consumed by methanotrophic microorganisms largely limiting emissions to the atmosphere however methane concentrations are often elevated in oxic surface waters of oceans and lakes methane paradox aerobic methane production in surface waters might constitute a particularly important source of methane which due its proximity to the atmosphere might escape the aquatic microbial methane filter yet we currently lack a comprehensive understanding of the involved processes and microorganisms moreover enhanced eutrophication of coastal ocean and lake ecosystems has been linked to increased methane emissions despite the immense importance of methanecycling microorganisms in controlling emissions from these systems we know remarkably little on how changes in environmental conditions affect their in situ activitiesthe methaniaq project addresses these knowledge gaps by 1 resolving and quantifying aerobic methane production in surface waters of aquatic ecosystems with different trophic states and 2 unravelling how eutrophication affects methaneconsuming microorganisms in water columns of coastal ocean and lake ecosystems to tackle these objectives i will use an innovative combination of approaches comprising in situ measurements of biogeochemical process rates manipulation experiments under controlled laboratory conditions and cuttingedge molecular methods to analyze microbial communities the proposed approaches will provide an integrated view from the scales of enzymes and microorganisms to ecosystemlevel processes spanning marine and freshwater ecosystems i expect this crossdisciplinary project to generate essential insights into methane cycling dynamics in aquatic ecosystems and their effect on the global climate
artificial photosynthesis to produce fuels and chemicals hybrid systems with microorganisms for improved light harvesting and co2 reduction

the photo2fuel project will develop a breakthrough technology that converts co2 into useful fuels and chemicals by means of nonphotosynthetic microorganisms and organic materials using only sunlight as energy source photo2fuels technology is based on the artificial photosynthesis concept and will use a hybrid system of nonphotosynthetic microorganisms and organic photosensitisers to produce acetic acid and methane using moorella thermoacetica bacteria and methanosarcina barkeri archaea strains respectively after optimisation and characterisation this hybrid nonphotosynthetic microorganisms with organic photosensitiser system will be placed into an auto sufficient photomicroreactor running exclusively with sunlight during the day the natural sunlight will be used and during the night artificial light will be used from previous stored solar energy in batteries excess sunlight this approach will guarantee the continuous operation of the photomicroreactor additionally a solar concentrator will be coupled to the reactor to maximise conversion and stabilise the production of fuels and chemicals even with variant solar flux the photo2fuel project will also investigate technologies for the separation of the main products acetic acid and methane and deliver solutions to achieve high separation efficiency the overall sustainability of the photo2fuels technology will be analysed including the environmental economic and social aspects lastly the market barriers and key stakeholders will be analysed from an enduser perspective aiming at advancing the technologys trl4 after the project completion and thus actively supporting the transition to a climate neutral europe by 2050
beyond the standard model coherent neutrino scattering at the european spallation source

coherent elastic neutrinonucleus scattering cevns a recently discovered process of neutrino interaction provides numerous opportunities to search for physics beyond the standard model sm the cevns crosssection much larger than for any other coupling results in a dramatic miniaturization of neutrino detectors however the faint signals generated fewkev nuclear recoils nrs require advanced detector technologies the unprecedented neutrino flux from the upcoming european spallation source ess provides the opportunity for a definitive exploration of all phenomenological applications of cevnsthe centerpiece of this proposal is the development of a new cevns detector technology cryogenic undoped csi scintillators monitored by innovative sensors the largest avalanche photodiodes produced to date in combination with stateoftheart waveshifters nanostructured organosilicon luminophores an array of seven csi crystals at the ess operating at 80 k and adding up to just 32 kg will provide an exceptionallyhigh signal rate of 12000 cevns events per year significantly surpassing the sensitivity of tonscale detectors at presentday spallation sources the signal output per energy deposited in this new hybrid device is the highest ever achieved with scintillators this provides a sensitivity to the lowest nr energies expected from cevns for which deviations from the sm are most evidentthis combination of detector technology and neutrino source will achieve the best foreseeable sensitivity to new particle and nuclear physics via cevns a first investigation of the response of highpressure xenon detectors to lowenergy nrs will also be performed for reasons of nuclear structure csi and xe are essentially identical in their response to cevns however the technologies and their expected systematics are entirely different their simultaneous use at the ess will provide a unique robust confirmation of any signatures of new physics encountered
virtual twins as tools for personalised clinical care

vital will deliver a comprehensive clinically validated multiscale multiorgan modelling platform that is driven by and can represent individual patient data acquired both in the clinic and from wearable technology the platform will create a virtual human twin for individualised and sexspecific optimisation of medical pharmacological or surgical interventional therapy for complex multifactorial cardiovascular disorders that have systemic impact and high risk of comorbidities of ao the kidney and brain systemic hypertension heart failure withwithout preserved ejection fraction and hemodynamically complicated atrial septal defects userfriendly interfaces cocreated with healthcare professionals will provide access to the technology the platform consolidates existing multiscale and multiorgan models of the heart lung and systemic circulation and their regulatory mechanisms and advances the stateofthe art by incorporating currently missing biophysicsbased physiologyunderpinned components kidneyrelated blood pressure control hormonal actions vascular regionspecific smooth muscle cell functionality and cardiac and arterial growth and remodelling mechanobiological mechanisms by the end of the project the platform will have been validated and tested in more than 200 patients across 5 clinical studies in france and the uk to optimise the treatment of patients with resistant systemic hypertension heart failure or atrial septum defects a unique aspect of vital is its focus on monitoring the mental health of patients to understand their expectations and reservations towards digital health technology vital technology will be compliant with and contribute directly to the vphi and edith virtual twin ecosystem this will further unlock the potential of new digital tools that intelligently combine the power of physicsbased multiscale models artificial intelligence and data to provide better health care for all
facilitating autonomy in astrodynamics for spacecraft technology

the faast project facilitating autonomy in astrodynamics for spacecraft technology is proposed for developing autonomous guidance and control algorithms for cubesats and other interplanetary spacecraft this project will be conducted with prof francesco topputo of the dart group at politecnico di milano polimi in italy which has expertise in deepspace guidance navigation and control gnc from its past and present involvement with esa projects including multiple cubesat missions the primary focus of faast is on autonomous orbit guidance and control for deepspace scientific space missions a challenging problem which currently must be handled by dedicated teams of engineers on earth which support the spacecrafts operations in particular this work is concerned with autonomous guidance and control in the vicinity of asteroids which presents a particularly challenging environment the faast project will contribute to benefit from and interface with the dart groups extrema project a 5 year 20212026 2m project funded by the european research council extrema is developing new gnc techniques for selfdriving spacecraft faast proposes research activity that spans from fundamental developments in celestial mechanics and astrodynamics to specific algorithm development and demonstration in a highfidelity setting this presents an ambitious course of research for a twoyear project but it is wellorganized into four achievable objectives the researcher holds a phd in aerospace engineering sciences from the university of colorado boulder a leading institution in spaceflight education and research the supervisor is a full professor of aerospace engineering at polimi and a leading expert in spacecraft gnc and interplanetary cubesats this project has the potential to meaningfully advance the stateoftheart in spacecraft guidance and control while facilitating transatlantic cooperation between the us and eu spaceflight research communities
novel metalorganic framework adsorbents for efficient storage of hydrogen

widespread use of hydrogen as an energy carrier is a key priority for the eu in order to achieve its climate and energy transition targets developing sustainable efficient and safe hydrogen storage technologies has however proved challenging mosth2 in full alignment with the requirements of horizoncl42021resilience0117 proposes an integrated multiscale labtotank approach to develop validate and demonstrate innovative low cost cryoadsorptive hydrogen storage using monolithic metalorganic framework mof adsorbents with an optimal combination of volumetric and gravimetric capacity but also a small environmental footprint advanced synthetic strategies and sophisticated computational techniques including molecular simulation and machine learning will be combined in a cyclic materials development approach to deliver new high performance sustainablebydesign mof adsorbents the main aim is to computationally design then synthesise and validate experimentally ultra porous mofs with usable storage capacities above 10 wt and 50 gl on a materials basis at an operating pressure below 100 bar this represents an essential step towards more efficient intrinsically safer and cost effective storage solutions compared to conventional hydrogen storage technologies an important part of the project will be devoted to developing and upscaling monolithic forms of optimal mof materials to allow easy integration into a cryoadsorption storage tank specifically designed for this purpose which will be tested in a trl 5 environment the outcomes coupled with full life cycle analysis and technoeconomic assessment of mosth2 technology with a view to selected end uses rail and road applications will form the basis for elaborating future market penetration plans through a solid horizontal dissemination and exploitation strategy
eco conversion of lower grade pet and mixed recalcitrant pet plastic waste into high performing biopolymers

ecoplastic is designed to provide a seamless route to resolving pervasive pet plastic pollution converting it to ecoplastic prototypes conversion of unrecyclable post use pet into new high performance bioplastics embodies the regenerative zero waste approaches found in nature where post use materials become the ingredients for new products and with unlimited cyclical use of materials it proposes a technological paradigm shift in recycling from the current zero to single digit circuits of recycling loops to a regeneration process providing a significant scientific step forward towards true circularity ecoplastic converts lower grade pet and mixed recalcitrant pet plastic waste into high performing biopolymers through the development of a suite of breakthrough modular and technologies adaptable to the waste input to funnel waste pet plastics into ecoproducts that enter a perpetually biocyclable loop the new ecoplastics and products will provide drop in alternatives for seamless adoption within industry and by consumers we will demonstrate that the resulting processes are economically environmentally sustainable for valorizing currently nonrecyclable materials such as multilayer packages and flexible films the project will combine several approaches to optimise the material circularityidepolymerization process series of mechanogreen chemical and biocatalytic technologies to depolymerize them into their constituent monomers using novel biological filtration for the preparation of highly fermentable monomer and oligomer feedstock streamsiibiopolymer production using microbiome processing to produce new biopolymers from monomer feedstocks which are then processed into bioproduct prototypes that are not harharmful to the environmentiiiadvanced processing will be used to advance the properties of recovered biopolymers and demonstration prototypes for applications including packaging will be produced
study on the influence of nitroxyl hno and nitric oxide no bioactivity on potato defense against pathogens on the lookout for improving the global food supply

potato solanum tuberosum l is one of the worlds most important crops for human consumption thus it is an integral part of the worlds food supply poland is the worlds 8th largest producer 2nd in the eu with 9 million tons annually potato is susceptible to a wide range of pathogenic organisms which can cause severe quality and yield losses worldwide given the absence of cultivars resistant to pathogens and the immunization of pathogen isolates to successive fungicides any attempt to understand and strengthen the knowledge of factors related to natural potato immunity is extremely importantin light of this it is well documented that nitric oxide no is a key element involved in pathogen recognition as well as activation of local and systemic plant defense signaling activities and pathways moreover the interdisciplinary group proposing this plan recently reported endogenous production of hno nitroxyl in plant tissues arabidopsis thaliana for the first time and points toward a novel regulatory role under physiological conditions hno concentration in plant cells is in the nanomolar range the ubiquitous hno bioavailability allows it to support or compete with no signaling depending on the local redox environment shifts with stress or senescence finally we showed that hno formation is an early reductive stressrelated responsetaking all this into account this proposal will focus on the realtime detection and visualization of simultaneously endogenous hno and no in leaves of the important crop plant challenge inoculated with vravr phytophthora infestans a cause of late blight disease moreover it will be an important issue to answer the question of whether and to what degree the hno presence in cells modifies the plants defense responses the hypothesis is that hno is generated during potato p infestans interaction and can act as a functional signal molecule favoring redox changes towards effective defense responses in potato