Dragon09/odtu_chatbot huggingface dataset:
elucidation of the biosynthesis pathway of the glaucoma drug pilocarpine in pilocarpus plants

pilocarpine is a plantderived alkaloid used to treat primary angle closure glaucoma and dry mouth to meet the commercial demand for pilocarpine the alkaloid is extracted from wild pilocarpus plants in the amazon rainforest excessive harvesting of wild populations has led to the official listing of several pilocarpus species as threatened synthetic biology offers a sustainable approach for pilocarpine production however to produce the compound in heterologous hosts such as yeast the biosynthesis pathway of pilocarpine needs to be elucidated first in pilosyn i aim to unravel the natural biosynthesis pathway of pilocarpine which is currently completely unknown my host group recently obtained transcriptomics and metabolomics data sets of two pilocarpus species and these will serve as the basis of the project as several pathway options are conceivable for pilocarpine biosynthesis i will first reduce the number of options by identifying the metabolic precursors of the pathway and by obtaining structural and spatial information about possible pathway intermediates then using the transcriptomics data sets i will identify the enzymes that catalyze each of the pathway reactions for this i will use a learningbyconstructing approach in transiently transformed leaves of nicotiana benthamiana the results of pilosyn can be used to establish a cheap and sustainable pilocarpine source in a heterologous host this will eliminate the need to harvest wild pilocarpus plants thus is preventing their extinction and protecting the fragile ecosystem of the amazon rainforest through pilosyn i will expand my knowledge on enzyme discovery and pathway elucidation and receive valuable training to become an independent and mature researcher in plant metabolism
a new strategy for chilldown enhancement in cryogenic propulsion systems

ambitious goals of total greenhouse gas ghg emission reduction and decarbonisation have been set by the recent policies european green deal energy union 2030 energy and climate targets and european unions 2050 longterm decarbonisation strategy aiming for a successful green energy transition my project objective is to enhance the cryogenic chilldown process to minimise liquid hydrogen consumption in future applications as fuel for terrestrial maritime and aviation transportation indeed combined with partial vehicle electrification the use of cryogenic fuels first and foremost liquid hydrogen in terrestrial maritime and aviation transports has gained an increasingly prominent role thanks to their environmentally friendly nature and ability to store the energy and control its release cryogenic fuels can be stored as gas or liquid even though cryogenic liquefaction requires energy due to typical low temperatures 120 k it is advantageous since it produces high fuel densities this makes liquified cryogenic fuels particularly suitable for the next hybrid transport systems however defined as the initial transient process of keeping the system adjusted to the low temperature cryogenic chilldown in pipelines of fuel storage and handling systems is still highly inefficient average quenching efficiency 39 i propose a new strategy for cryogenic chilldown enhancement by tuning the inner wettability of pipelines using surface engineering via femtosecond laser texturing
art in border practice and policy

the projects main objective is to build a framework examining how artistic practice can be applied within migration policy contexts addressing calls by refugees asylum seekers and human rights organisations for more humane approaches to policy this framework will specifically investigate how artworks made in response to the eu migration crisis can raise awareness build understanding or disrupt usual relationships between refugees local longterm residents ngos and border security personnel developed within a comparative study between greece and tunisia the project will reflect the impact of the eus current retracting and expanding borders in two key sites on either side of the mediterranean sea as the eu enacts buffer zones to stem the arrival of asylum seekers on mainland europe art practices will be analysed for the ways in which they express and alleviate multiple stresses for those impacted by such policy beginning with a review of artistic practices on site in these two locations the project will map and analyse how art reveals relationships between multiple actors across refugee resident ngo and border personnel communities this review will be contextualised by a wider review of art in global border practices interviews with key informants and observations of different practices in greece and tunisia will then inform the development of a practice as research methodology drawing on the ers own arts practice to cocreate a series of artworks with local actors to test and map possibilities for new and different relationships between those enacting and those impacted by migration policy this participatory arts practice will iterate through a regular coevaluation process to a build a framework that reflects the role of art in relation to multistakeholder support partnership building and ethical approaches to interpersonal relationships the framework and its development will be made available on an interactive website
a leap in ligand technology the development and valorization of novel chiral diphosphine ligands

the search for innovative and efficient chiral catalysts and ligands for producing enantiopure molecules remains a vital priority across the pharmaceutical flavours and fragrances and agrochemical sectors a significant majority of newly developed drugs are chiral creating an increasing demand for advancements in asymmetric synthesis and the development of chiral ligands and metal complexes which is driving substantial progress in asymmetric catalysis despite these advances the number of chemical transformations required to produce chiral molecules continues to grow while only a small fraction of the hundreds of chiral ligands developed for use in chiral catalysts have been commercialized and even fewer have been successfully scaled for industrial applicationsthe most successful ligands often referred to as privileged phosphine ligands have achieved notable success in various transformations however their structural diversity is limited constraining further expansion of reactivity and their production costs remain high most of these successful chiral phosphine ligands require multistep timeconsuming and costly noncatalytic synthetic routesin this context this proposal aims to build upon a recent breakthroughthe discovery of a completely new class of highly efficient chiral phosphine ligands notably these ligands offer a significant advantage by being producible via a robust catalytic synthesis that requires only 12 steps moreover the structure of this new ligand family is highly modular allowing for rapid diversification and the creation of a library of related ligandsthese promising features suggest that our discovery holds the potential to develop a product with a compelling value proposition representing a significant advancement over current competing solutions in the market
exosome analysis business for inclusive innovation ecosystem in eu

exit071 exosome isolation tool is a leiden based startup developing pioneering technology for the analysis of novel biomarkers exosomes we are a young yet experienced startup with great motivation and ambition to bring a very much needed technological solution to a highly potential exosomes market this funding opportuning women techeu from horizon europe comes at the perfect time for exit071 the technology is already proven to perform plan partners and finances for technological development are secured and the team is coming together in this project exo4eu exosome analysis business for inclusive innovation ecosystem in eu we propose activities for indepth makeover of our business plan and strategy enhancement the networking opportunities offered by investeu and the enterprise europe network are very appealing for our early integration into the european ecosystem of innovation being mentored and coached under the women leadership programme will have a bespoken value for the passionate founder and quickly learning ceo dr yuliya shakalisava who is making a challenging yet exciting career transitioning from research to business
solid rapid and efficient adoption of data ai robotics applications in production

solidair brings together 10 manufacturing technological and research experts to accelerate the uptake of artificial intelligence ai and robotics in european manufacturing using data as an enabler it will codevelop and demonstrate tailored solutions to digitalise and automate visual inspection and physical testing enable predictive quality control and process optimisation ai robotics systems are not extensively used in the production industry because it is not clear whether they are safe and when or why they will fail the solidair project tackles this problem by researching developing and testing methods that are as solid and trustworthy as possible to be adopted by the european industry while being costefficient to develop and replicate new methods and tools will be developed by research and technology providers which leverage the current state of the art in visual ai ai for process data and smart collaborative robotics the developed technologies will be applied and demonstrated in 4 industry use cases to prove their functionality and applicability in real production environments the objective is to improve production processes through digitalised and automated quality control for high volume high rate and flexible manufacturing the use cases are led by mature european manufacturing companies and represent problems that every producing industry struggles with solving them increases the process efficiency and flexibility while improving the working conditions of the process staff the developed methods shall be efficiently and easily adaptable and replicable so they can be easily applied to new use cases outside the consortium the accelerated adoption of the ai robotics solutions tools and methods solutions will facilitate a strong increase in the competitiveness and sustainability of the eu manufacturing companies across sectors
advancing global smeft fits in the lhc precision era

after more than a decade of datataking no direct evidence of new particles has been found at the lhc suggesting that new physics np might be heavier than the energies currently probed heavy particles can alter interactions among known particles causing subtle deviations from the standard model sm predictions the standard model effective field theory smeft is a robust framework that allows for a modelindependent parametrisation of new interactions providing us with the tools needed for a successful indirect discovery program in particular the peculiar structure of the theory dictates correlations between different observables motivating global analyses in this proposal i develop a strategy to advance global smeft interpretations in three key directions firstly i will expand the comprehensiveness of the dataset by including lowenergy observables in current global fit methodologies to accomplish this renormalization group equations mixing effects must be included in theoretical predictions as they prescribe how different energy regimes are connected secondly the research project aims to advance the design of optimal observables for global fits by using machine learning techniques with the goal of maximising sensitivity to np lastly the ultimate objective of indirect searches is the identification of heavy new particles responsible for the modified interactions the smeft is simply an intermediate step in this endeavour i will provide the particle physics community with an opensource software that will interface with the output of global smeft fits and indicate which heavy particles are disfavoured by the data and which are still viablethe combination of my expertise in smeft analyses and collider phenomenology along with the host institutes proficiency in advanced statistical methods flavour physics and uv matching provides an ideal setting to successfully execute the proposed tasks and significantly advance indirect searches
reactive adaptive behaviour elicited by events in close proximity of the body

our perception of the surrounding world is intrinsically related to the motor system in any natural environment animals become adept at sensing sudden transient events and adapt their behaviour accordingly in order to survive we are often exposed to sudden sensory stimuli which irrespectively of their sensory modality evoke a series of fast modulations of muscular activity named corticomuscular resonance cmr this type of reactive adaptive behaviour rab may be strictly connected to the distance between us and the surrounding sensory stimuli how does the brain cope with transient events eg threats in close proximity of our body the notion of peripersonal space pps has been introduced 40 years ago to highlight the link between somatosensory and visual processes related to the space immediately around the body whatever can be seen or touched in close proximity to our body inevitably affects our behavioural responses in terms of reflexes or voluntary actions this proposal aims for the first time to systematically explore how reactive adaptive behaviour ie cmr is affected and modulated by transient sudden stimuli or events within the pps the proposed experimental setup will have the advantage to resemble reallife ecological environments here we have also the unique chance to use a combination of techniques such as highdensity eeg recordings hdeeg behavioural techniques ie psychophysics electromyography emg eye and motioncapture on both primate and nonhuman primates moreover we will have the rare opportunity to perform intracranial electroencephalography ieeg in nonhuman primates by recording from brain cortical structures which are fundamental in planning and organizing movements eg premotor areas
space qualified gan components for next generation systems

the main objective of sgannext is to develop a fully european gan on sic foundry process and demonstrate outstanding performance at high frequency beyond qband through the design of efficient and robust sspa lna and switch devices for flexible leogeo payloads for this purpose the project led by sener as satellite equipment manufacturer includes an epitaxy manufacturer swegan an industrial foundry ums a research foundry fbh and two universities unibo and uab moreover the consortium count on the two main european satellite prime contractors ads and tas for the conceptual definition of services and the required system to answer market demandsgannext aims to secure a european supply chain with gan epitaxial wafers provided by swegan for this new process qv band power cells will be designed making use of novel processing modules and epitaxial concepts which reduce parasitic losses and increase thermal drain to heat sink in parallel ums provides access to its 01m gan technology gh1010 which will be optimized and submitted to a space qualification assessment through two runs available for mmics design and validation microwave characterisation of gan technology performance by model refinement and device characterisation will be addressed to improve mmic design process along the projectas highly efficient pas are essential for telecom active antennas with high number of active units at least three pas design concepts are proposed to answer the needs identified at equipment level the efficiency has a critical impact on the extra power demanded to the system and the increased complexity to dissipate on the reception side a design of a lna as well as a switch for robust rf frontend will be addressed last but not least packaging techniques will be evaluated for space use and finally a demonstrator of an sspa for actual antenna systems based on the designed mmics will be developed and tested under space environmental conditions
the rediscovery of catacombs between the 16th and 20th century in the mediterranean basin

this project will trace the history of the rediscovery of ancient catacombs between the 16th and 20th centuries across the mediterranean from a broad european perspective through studies conducted on travel influx exploration archive and museum collections catacombs are a valuable and cultural evidence of the transition from antiquity to the middle ages in the mediterranean basin however there are currently no systematic analyses on their rediscovery since indepth investigations have only been carried out on a local scale the study will fill this gap and maintain a strong interest in museum and collection history which so far has been greatly underrepresented in research no studies have focused on the role played by catacomb archaeology from a perspective that will assist in building a european common culture undergroundmed will trace the history of the rediscovery of catacombs by a vast number of scholars explorers and others during the aforementioned periods the research will focus on various countries using a multidisciplinary approach combining archaeological surveys museum studies and archival investigations to highlight the impact of these rediscoveries in european culture and its scientific excellence the project will open the door to debate in regard to the historical definition of a shared european identity in a borderless europe moreover it will define the roles that european scholars had on the creation of an international and multicultural researchnetwork while travelling across the shores of the mediterranean in search for catacombs ultimately the fellowship will greatly contribute towards the researchers academic career through networks research experience and dissemination activities within the host institution together with the selected secondment the relevant transfer of knowledge with the host institution will also greatly contribute towards the researchers career progression
fathoming sequestration and enrichment of metals in deep marine deposits with novel microxray emission spectroscopy

rare earth elements ree and transition and posttransition metals tm are essential to modern life yet we know little about how they concentrate at earths surface especially on the seafloor which holds vast reserves the deepsee project will shift paradigms on marine metal deposits from chemical composition and resource inventories to a holistic view based on atomicscale observations and modeling this research will determine geochemical processes that give rise to some of the highest metal partitionings in supergene ores first crystal chemistry of 3 ree in biogenic vs authigenic sedimentary apatite is proposed as a new proxy for the paleoceanographic enrichment setting with the potential to become an indicator for future exploration sites second crystal chemistry of 3 ree will elucidate the scavenging history of femn crusts and corresponding evolution of seawater ree as recorded in growth layers over millions of years third investigation of the redox chemistry and mineralogy of femn crusts and nodules will explain how the redoxsensitive metals co ce tl and pt are enriched from 109 to 106 times relative to seawater to date these processes have been impossible to interrogate because of analytical challenges posed by the multielemental composition and mineralogical heterogeneity of seafloor deposits a promising approach for tackling these challenges is new microxray emission spectroscopy using a unique highluminosity compact xes spectrometer at the european synchrotron radiation facility installation of the spectrometer on a microfocus beamline under construction on the new 4th generation esrf xray source will provide a momentous gain of at least 100 in detection limit and unprecedented sensitivity and precision in the analysis of ree and tm more broadly the research will show how new knowledge about earth processes can be obtained with a fresh look at individual trace elements previously inaccessible by crystal chemical study
engineering of bacteria to see light

eos wants to create a new class of biohybrid living materials capable to perceive light and perform tasks remotely ie drug delivery in hardtoreach body locations i propose to achieve this by inducing light sensitivity into nonphotosynthetic motile bacteria at minimum invasiveness and complexity based on the notion that the active manipulation of the bacteria membrane potential allows controlling bacterial functions including motion eos challenge is how to trigger effectively a membrane potential dynamics eos will employ a materialsbased approach that i call optobacterialstimulation and is composed by two key ingredients i light as a spatiotemporal precise tool that can control bacteria remotely ii phototransducing organic materials that associate spontaneously with bacterial cells without the need for neither covalent attachment nor genetic modification the phototransducing mechanism stems from the crosstalking between molecular excitations and the polarization state of cells the photoinduced membrane potential dynamics is ultimately linked to ion motive force and thus to the bacterial flagellar motor all these abovementioned elements have in common the study of lightmatter interaction which represents my main research interest in the last decade using optobacterialstimulation i will answer to three outstanding questions that are related to each other and shape eos objectives i can we engineer bacteria to perceive light through exogenous phototransducers ii are bacteria able to perceive different photostimulation approaches as dictated by molecular excited states in terms of membrane potential dynamics and motion iii can we understand the relationship between the fate of excited states and bacterial function in the case study i will demonstrate phototactic guidance of eyeless bacteria that are competent to swim in the gastrointestinal tract with the view to develop intrinsically biocompatible microswimmers
receptor ectodomain shedding in plant immune signal transduction

receptor ectodomain shedding shedding is a fundamental mechanism of the regulation of communication between mammalian cells shedding refers to the proteolytic cleavage of the extracellular portion of a membrane protein this process can inactivate the function of a receptor generate extracellular signalling molecules and trigger the generation of intracellular protein forms involved in signalling shedding is best understood in mammals with hundreds of shedding events reported but also occurs in other animals and fungi a few cases of shedding have been observed in plants membrane receptor like kinases rlks constitute the main players of celltocell communication in plants with many of them serving as key immune receptors rlk shedding has been reported in two occasions pointing to an important regulatory function despite the essential functions of shedding in animals the biological significance of rlk shedding in plants and the fact that plant cells harbour many more receptors than their animal counterparts shedding has yet to be studied systematically in plants by reanalysis of large scale extracellular proteome datasets i found evidence of widespread rlk shedding events in plants however this analysis could not reveal exact cleavage sites that are important for follow up functional studies trough resist i seek to develop this new field of ectodomain shedding in plant biology and study the mechanisms of regulation and the consequences of plant rlk shedding to do so i aim to establish a platform for experimental identification of shedding events in plants by adapting cutting edge methods recently developed in medical sciences for precise identification of processed membrane proteins and released ectodomains in nicotiana benthamiana i will use this platform to analyse the plant sheddome the repertoire of shed proteins and its dynamics upon defence activation and to identify interesting shedding events for functional characterization