Dragon09/odtu_chatbot huggingface dataset:
cell cycle progression in malaria parasites

all eukaryotic cell multiplication requires wellorchestrated developmental programs and regulatory pathways to guarantee fidelity in transmission of genetic information multiplication inside red blood cells of plasmodium falciparum the deadliest malaria parasite is responsible for malaria pathogenicity unlike model organisms plasmodium divides in unconventional ways producing not two but up to tens of thousands of daughter cells in a single cell cycle round this points to a yettobeexplored original and divergent cell cycle architecture where conventional rules likely do not apply we hypothesise that a transcriptional clock paces the cell cycle while a network of local players modulates and finetunes the activity of effectors through phosphorylation to test this hypothesis we will first use single cell transcriptomics and high resolution phosphoproteomics to understand how these are connected with cell cycle events and their contribution to normal progression and controlled cell cycle arrest secondly we will conduct a genomescale conditional genetic screen to identify cell cycle regulators we will map progression of pooled barcoded mutants using cell cycle reporters barseq and single cell transcriptomic readouts finally we will combine the data collected throughout the janus project and provide an integrated model of cell cycle progression checkpoints their associated transcriptional and signalling events and their interdependence furthermore we will functionally dissect on a genebygene basis the entrance into the replicative phase based on our modelled data altogether the janus project will provide insights into an ancient yet divergent process essential for parasite survival and propagation with unprecedented detail it may reveal innovative eukaryotic adaptations to cell cycle control in this basal lineage which could generate new insights into protist biology and provide new tools in the continuing fight against malaria
climate risk and vulnerability assessment framework and toolbox

many european regions and communities have limited experience and resources to integrate available local and global data models and concepts into a contextspecific climate risk assessment cra for an authoritative cra local data and evidence need to be integrated with established paneuropean reference data and scenarios climaax will provide substantial financial analytical and practical support to regions and communities to develop and improve their cras by a number of activitiesclimaax will deliver a a standardized cra framework cra built on current community experience and bestpractices b a toolbox with data models and utilities to provide access to european and global open data archives integrated with local data and procedures c five european pilot regional cras to shape the framework and toolset d financial support for at least 50 regions to execute a contextspecific cra e cra guidance material and online helpdesk for other european regions and f a proposal to upscale results into the future operationalization of the regional cra support functionthe main target groups are regional authorities community organizations sectoral stakeholder organizations ngos citizen networks and civil protection agencies focusing on climate risk management and emergency response the project will engage a large community of practice to cocreates the framework and toolbox and codefine the financial support application and monitoring criteriathe main outcome will be a demonstrated acceleration of the design and implementation of regional risk management plans for climate adaptation and emergency response
development of personalized and affordable multisubstituted calcium phosphate based scaffolds for bone augmentation applications

less than half of the global population is covered by essential healthcare services and the aim of the postdoctoral fellowship project affordbones is to obtain personalized and affordable scaffolds for bone augmentation procedures dr antonia ressler applicant will study scaffolds obtained by using 3dprintable calcium phosphate multisubstituted with ions strontium magnesium and zinc crucial for efficient bone regeneration stereolithography as an additive manufacturing technique is going to be used for printing precise highly porous scaffolds based on multisubstituted calcium phosphates for bone augmentation procedures in collaboration with the company lithoz after the detailed analysis of physicochemical properties of printed scaffolds in vitro and in vivo biological properties will be obtained to determine the scaffolds osteogenic properties and the influence of substituted ions on bone regeneration by using stem cells from female and male donors and female and male rats respectively further company planmeca will provide patient real cases and the needed technologies for obtaining a demonstration of a personalized scaffold customized to the patient complex jaw bone defect the project will be carried out at the faculty of engineering and natural sciences and the faculty of medicine and health technology of tampere university under the supervision of prof erkki levanen and cosupervision of prof susanna miettinen affordbones project requires a high level of interdisciplinary work and the project is planned bearing in the mind that the improvement in the field of bone regeneration relies on bringing together experts from different fields who are nowadays distant from each other such as material engineers biologists clinicians surgeons and related companies
knowledgeable comprehensive and fully integrated smart solution for resilient sustainable and optimized transport operations

the overreaching goal of keystone is to support the development of a sustainable efficient and safe transport system allowing enforcement authorities to access data for the purpose of checking compliance with rules applied in the transport of goods and passengers the aim is to tailor standardised digital solutions that can be used from several realities to standardize the transport system to demonstrate the validity of the solutions proposed an app will be developed so that two highly diverse pilots can prove the efficiency of the keystones innovation using the gained experience to develop a seamless interoperable and intermodal digital transport ecosystem that can be replicated at european levethe overreaching goal of keystone is to support the development of a sustainable efficient and safe transport system allowing enforcement authorities to access data for the purpose of checking compliance with rules applying in the transport of goods and passengers the aim is to tailor standardised digital solutions that can be used from several realities in order to standardize the transport system in order to demonstrate the validity of the solutions proposed an app will be developed so that two highly diverse pilots can prove the efficiency of the keystones innovation using the gained experience to develop a seamless interoperable and intermodal digital transport ecosystem that can be replicated at european level the keystone digital solutions consists of standardised apis for data and information sharing between transport enforcement authorities and logistics operators based on a federated approach allowing to reduce the costs of logistics thanks to more efficient operations and interoperability to reduce the impact on the environment thanks to data sharing the possibility to consolidate flows and to improve safety thanks to seamless information exchange with enforcement authorities and the possibility to foster the acceptance of the ccam solutions
humancentered trustworthiness optimisation in hybrid decision support

themis 50 draws researchers and practitioners from diverse disciplines in order to secure that aidriven hybrid decision support istrustworthy and takes place in accordance with the particular human user needs and moral values as well as adhere with the keysuccess indicators of the embedding sociotechnical environment it implements an aidriven humancentered trustworthinessoptimisation ecosystem that users can use to achieve fairness transparency and accountability in themis 50 the trustworthinessvulnerabilities of the aisystems are determined using an aidriven risk assessment approach which effectively translates thedirections given in the trustworthy ai act and relevant standards into technical implementations themis 50 will innovate in itsconsideration of the human perspective as well as the wider sociotechnical systems perspective in the risk managementbasedtrustworthiness evaluation an innovative aidriven conversational agent will productively engage humans in intelligent dialoguescapable of driving the execution of continuous trustworthiness improvement cycles themis 50 adopts the european humancentricapproach to the design development deployment and operation of the themis 50 ecosystem and in this respect themis 50 willbase the implementation of its aidriven ecosystem on strong cocreation processes themis 50 will pilot and evaluate the humancentricecosystem using 3 welldefined use cases each addressing a specific high priority and critical application and industrialsectors the themis 50 solution enhances and accelerates the shift towards more trusted aienabled services by unlocking the powerof humans to evaluate the trustworthiness of ai solutions and provide feedback on how to improve the ai systems users can nowbetter challenge ai systems pinpoint any biases or problems embed their own values and norms and provide feedback to aidevelopers and providers for improvement
mediterranean women and sexual violence in the confessional 16001750

i did not accuse him because i am his enemy but because i demand justice he raped me and then said i should have married his nephew lorenzo to cover this the pregnancy up maria taboni v don gaucihe groped me asking for my pubic hair and a pezza cloth damped in menstrual blood i was horrified margherita caruana v don fabbrithis work is inspired by all the womens and young girls voices found in the inquisitorial records they recounted assaults and harassment with vigor and supported one another in seeking justice they condemned the predatorily actions and the system of impunity their disturbing depositions convey an outstanding narrative they show how women can be vocal and no longer take the blame for sexual violence against them to date their voices have been neglected by the historiography on sexual violence and the church in mediterranean cities women and children often endured sexual violence whilst confessing to their priests hundreds of reports about assault and abuse were sent to the roman inquisitors between 1600 and 1750 however what is remarkable is that the survivors and their families stood up to the perpetrators and called for punishment irrespective of the clergymens social standing despite the power dynamics at play and the gender roles women took an active role in defending themselves both by opening up to their families and with other females support speaking up to the authorities the inquisition took the denunciations seriously and demanded the culprits be questioned and punished unlike what we might expect mothers husbands fathers and friends were overwhelmingly supportive by corroborating the assaults in court as such women were able to circumscribe the authority of the church and its abusive men they did not endure abuse quietly they spoke up and demanded justice
unveiling the origins of whitedwarf explosions

the explosive deaths of white dwarfs are essential in heavy element nucleosynthesis galaxy feedback and for understanding the evolution of binary systems type ia supernovae sne ia are famous for their key role as cosmologicaldistance indicators and in the discovery of the accelerating universe recent highcadence surveys and theoretical advances have hinted that sne ia may not be the dominant way in which white dwarfs explode with the potential existence of large populations of faint and rapidly evolving whitedwarf explosions these unexplored classes of exotic transients may come from white dwarfs being torn apart by intermediatemass black holes mergers with neutron stars or collisions in triple systems but the mapping between explosions and observed transients is undetermined this project aims at providing the first complete census of the multiple ways that white dwarfs explode by mapping their observations to their explosion physics and constraining their diversity this sample will be crucial for defining optimal samples of sne ia for cosmology as well as determining the rates and contributions of whitedwarf explosions to the origin of the elements we will achieve this by i obtaining the largest ever rapidly discovered and spectroscopically confirmed sample of whitedwarf transients with detailed followup observations ii the application of machinelearning techniques eg neuralnetwork emulators for rapid comparison to sophisticated explosion models and iii the determination of the rates and diversity of their explosions in different galaxy environments the confirmation and exploration of the multiple explosion channels for producing normal sne ia and exotic whitedwarf transients will have major implications for their nucleosynthetic yields their use in cosmology as well as predicting rates of double white dwarfs that will be detected in huge numbers in the milky way by the gravitationalwave detector lisa
behavioral statedependent effects of electrical stimulation on neuronal activity

techniques to deliver electricity to the human brain have been applied in an attempt to restore brain functions or modify behavior yet many fundamental questions remain first it is unclear how to design stimulation protocols that lead to reliable stimulation outcomes second it is challenging to define a stimulation protocol that could modify behavior without a clear understanding of the dynamic interplay between brain regions during complex behaviors finally one of the goals of stimulation is to induce effects that outlast the stimulation period but whether and how lasting effects are induced is still unclear as a first step to tackle some of these issues the proposed project aims at understanding how electrical stimulation affects the activity of neuronal population invivo and whether behavioral states modulate such effects here i plan to use mice to bridge neuronal and behavioral levels combining highly controlled behavioral paradigms with recordings of neuronal activity at high spatial resolution during electrical stimulation applied using cuttingedge technologies i will also implement computational models to understand the mechanisms of action of electricity on neuronal activity and to guide further experiments insights from this work will shed light on the realm of possible effects of electrical stimulation and on how brain activity could be shaped to achieve specific behavioral outcomes ultimately this knowledge may lead to the rational design of stimulation protocols that could be applied in humans ideally noninvasively to restore brain functions
research to reality digital solutions to european challenges

the event organised by the belgian regions represented by the department ewi from the flemish government innoviris from the brussels capital region government and spw economie from the walloon government aims to bring together researchers involved in research on digital technologies within the horizon europe programme implementers involved in projects from the digital europe programme and policy makers working on digital policy at regional national and european levelsthe primary goal is to discuss policy and practical challenges hindering cooperation between the two framework programmes particularly in terms of digitalisation to ensure diverse perspectives there is a strong emphasis on making this event interactive other objectives include fostering networking among different target groups reviewing the functioning of the digital europe programme and providing a platform to showcase best practices in cooperation and synergy between the two framework programmesthe event is scheduled for the 5th of february 2024 in brussels with visits to relevant organisations in belgium planned for the following dayoverall the event aims to create a dynamic platform where researchers implementers policy contributors and other stakeholders can converge exchange ideas and contribute to the advancement of digitalisation initiatives in europe
visualising neuronal signalling dynamics within intact neuronal circuits deciphering the role of cellspecific mecp2 dynamics in neuronal function and dysfunction

epigenetic signalling pathways are required to translate external sensory input to neuronal gene modulation and function and disruption of epigenetic signals leads to devastating brain pathologies one prominent example is rett syndrome rtt a postnatal neurodevelopmental disease which results in rapid deterioration of sensory motor cognitive and social functions rtt is caused by loss of function mutations in a single gene encoding for methylcpg binding protein 2 mecp2 an abundant and multifunctional methylation reader in the brain while transgenic mouse models of mecp2 loss have provided significant insights into rtt they also revealed the vast complexity of the regulation of mecp2 signalling and cell specific heterogeneity importantly both a deficit and a surplus of mecp2 give rise to pathological phenotypes here we will develop an imagingbased approach to monitor endogenous mecp2 signalling and dynamics in intact neuronal circuits in awake behaving mice we will combine crisprcas9 genome editing to fluorescently label endogenous mecp2 with fret based biosensors to detect dynamic mecp2 signalling without perturbing its innate regulation in vivo twophoton fluorescence lifetime imaging will enable dual imaging of mecp2 signalling and concurrent neuronal activity using this approach we will map the functional landscape of mecp2 activity in the intact mouse brain across celltype circuit and sensory experience we will also image in vivo mecp2 signalling in transgenic mice with common rtt mutations this will allow us to detect early celltype specific mecp2 dysfunction and avoid broad latestage rtt symptoms the direct visualisation of the intricate interplay between mecp2 signalling and neuronal function within intact neuronal circuits will be transformative since it will shed light on the physiological role of epigenetic signalling in the brain and provide vital insights to future therapeutic interventions
decentralised cloud labs for industrialisation of energy materials

decode aims at creating and demonstrating a decentralised and adaptable future lab concept that connects multiple labs on a single platform in order to boost the effectiveness and speedup the development and innovation path for clean energy materials and technologies initially demonstrated for selected hydrogen technologies the decode platform is expected to find wide adoption in the clean technology field in the longer run including energy harvesting conversion and storage clean water technologies and the synthesis of valueadded chemicals and fuels the core of the platform comprises three elements the decode fabric that connects adaptative multiscale modelling and characterisation suites in a matrixlike structure a scoring concept to assess modelling and characterisation suites in terms of their integration readiness level irl and an aienabled central unit cpu that processes the irl scores performs the technology mapping to the fabric and orchestrates contributions in modelling and characterisation from partner labs for the platform as a whole decode strives to achieve a high level of flexibility adaptability and interoperability in terms of materials modification strategies technologies and operating regimes that it will be able to handle water electrolysis and hydrogen fuel cell technologies are selected for the demonstration of decodes decentralised labs platform the project will join leading expertise and capabilities in physical theory and modelling design fabrication operando characterisation and testing of functional materials and components materials digitalisation and cloudconnected lab operations and industrialgrade component integration and inlineendofline testing and validation by industrial partners in the consortium
modernisation of agriculture through more efficient and effective agricultural knowledge and innovation systems

modernakis aims to improve akis actors capacities to leverage individual organizational and systemic resources needed for the transformation towards more coherent effective and efficient akis systems and the transition to a more sustainable management and use of natural resources in farming and forestryto this end it will build and foster a european network of at least 1000 key akis actors including akis coordination bodies from all eu ms who will act as linchpins in the transformation of the akis systems towards a more effective governance and the modernization of the european agrifood sector the project will also build the capacities of these key akis actors towards systems understanding and engagement enabling them to enact longterm system changes that will improve the akis in addition it will set up at least 1 community of practice in each member state enabling thus participants to act as vectors of change in their communities in addition modernakis will provide a comprehensive digital catalogue with new knowhow and at least 80 tools and methods supporting key akis actors to improve the knowledge flows and develop a wellfunctioning akis in line with relevant policy objectives eg the green deal farm2fork sdgs
unified architecture for open ranenabled distributed scalable and sustainabilityenhanced 6g networks

unity6g aims to create a highly sustainable and scalable integrated ainative architecture that can support the diverse requirements of 6g networks by relying on advanced technologies such as distributed ledger technology semantic communications digital network twinning to enhance the performance costefficiency and trustworthiness of integrated 6g network services and applications the focus is on scalability and sustainability for integrated networks nonterrestrial and terrestrial networks xhaul open ran nonpublic networks edge core and cloud furthermore unity6g aims to evolve to realtime distributed and network stateaware open ran that can leverage the integration of distributed applications in the integrated architecture this will enable finegrained datadriven management and control via incorporating dapps distributed applications that complement existing xappsrapps and use cases with stricter timing requirements in an integrated network common interfaces and protocols will be defined so that different heterogenous domains can communicate seamlessly to better guide the design unity6g will use the principles of servicebased architecture for integrated networks and leverage digital twins for network evaluation unity6g considers four use cases targeting i sustainable networks for disaster handling ii immersive experience with realtime xrholographic communications iii digital twin for integrated 6g network evaluation iv multirat oran enabled npn for supporting time sensitive applications for industry 40