Dragon09/odtu_chatbot huggingface dataset:
lightsheet brillouin nanoscopy mechanosensitive superresolution imaging for regenerative medicine

better microscopes have always triggered scientific discovery lightsheet microscopy and nanoscopy are no exception and have initiated knowledge jumps in structural and dynamical imaging however they do not inform us on mechanical properties the domain of mechanosensitive microscopy is still in its infancy yet has already unveiled a stark dependence of cellular development on local stiffness and viscoelasticity for instance coordinated strain on the submillimetre scale is a key ingredient to grow induced pluripotent stem stells into a beating adult cardiac muscle without such an environment a twitching heap of cardiomyocytes develops instead what are the processes within cells that cause this forked differentiation how can we optimise the growth of artificial tissue in regenerative medicine given the dynamics and 3d nature of the problem paired with the requirement of subcellular resolution one must conclude that our current instrumentation is not up to the task thus this project aims to develop a labelfree microscopy technique that can image viscoelasticity at unprecedented subdiffraction resolution inside living differentiating cardiac tissues at orderofmagnitude faster acquisition speeds than previously possible this will be made possible by a completely new type of optical element that allows snapshot hyperspectral imaging at unparalleled speed and sensitivity transforming latest innovations within nanoscopy and lightsheet imaging and using brillouin scattering as a proxy for viscoelastic tissue properties on the microscale lightsheet brillouin nanoscopy librina will be the fastest most gentle and highest resolution mechanosensitive microscope ever built besides being an enabler technology for cellular biology and regenerative medicine the project will explore new principles in labelfree nanoscopy methodology and initiate innovation jumps in optical instrumentation
cargotowing highly enantioselective electropumps unconventional asymmetric readout and transmission of chiral information

chirality is found throughout nature but it occupies a special place in chemistry perhaps for historical reasons but mainly as a result of the beneficial properties of chiral molecules across a diverse range of areas from medicine to materials science the global aim of cheir is to achieve with unprecedented efficiency the propagation of chiral information along different length scales based on the synergy of wellchosen molecular ingredients and physicochemical engineering the artificial systems here involved can be considered as models for the transmission of chiral information through space such ambitious aim could be achieved developing a cargotowing electropump based on chiral conducting polymers for targeted drugdelivery applications the combination of four main ingredients electric field and magnetic field externally applied electrical conductivity and enantiodiscrimination capability intrinsic features of the object makes these innovative miniaturized bipolar soft pumps perfect candidates for a multipurpose asymmetric detection in such scenario inherent chirality can provide the breakthrough it implies chirality and key functional properties to originate from the same structural element endowing the selector with extraordinary chirality manifestations that can be propagated from molecular level to the macroscopic one unprecedented recognition in terms of energy differences was recently observed implementing inherently chiral materials as enantiopure electrode surfaces recently attractive potentialities of these systems were also exploited in the field of bipolar electrochemistry and in the one of autonomous swimmers allowing to correlate the output signal with the concentration of the enantiomers present in solution such striking performance will prepare for the development of innovative systems with high impact in analytical biological and pharmaceutical fields considering the project interdisciplinarity
generation and validation of a panel of emt reporter cell lines from solid tumors

cancer is a very heterogeneous disease and its subtypes display great variability in their response to treatment the high failure rate of anticancer drug discovery consumes billions of dollars annually contributing to the high cost of those few drugs that are eventually approved one of the problems lies in inaccurate and oversimplified cell line models for studying human tumors yet a method to incorporate tumour subtype contribution in response to drugs is lacking to improve experimental oncology and drug discovery we propose to develop a validated toolkit of readytouse cell lines and defined cell culture media in order to clearly and unambiguously represent well conserved solid tumor subtypes this approach is drawn from our erc stgfunded project in which we invented validated and patented a method to genetically label any cell type or cell state transition our idea is novel because it uses custom synthetic genetic reporters in in vitro tumor models which enables tracing of tumor cell states the first component of the kit is a set of genetically modified cell lines representing tumors from different epithelial tissues and reporting on epithelial or mesenchymal states work package1 wp1 thereafter we will use these cells to define the formulation of two additional components of the kit i the cell culture media and ii the supplements to in vitro propagate and discriminate the two distinct cell states wp2wp3 in wp4 we will perform a product comparison by measuring the features of the toolkit cell lines in response to anticancer treatment as compared to current standards in parallel wp5wp6 will address industryquality ip strategy implementation and freedomtooperate given the socioeconomic impact of cancer and the lack of a similar technology our solution has the potential to eliminate the high failure rate of anticancer drug discovery because it will make it possible to perform drug screens on different tumor cell states at once
decoding the path to cellular variation within pathogen populations

heterogeneity amongst isogenic cells is pervasive throughout biology recently developed singlecell omics approaches are beginning to systematically reveal the repertoire of functionally distinct cell subpopulations within metazoan tissues pathogens frequently encounter changing and often hostile environments to adapt to these challenges unicellular pathogen populations also exhibit a large degree of celltocell heterogeneity which often affects the outcome of infections yet despite the importance of this celltocell variation very little is known about the mechanisms that control the level of heterogeneity in pathogen populations or why some isogenic populations are more heterogeneous than others the goal of switchdecoding is to unveil the path to cellular variation to this end i will go beyond identifying and describing new subpopulations of cells and elucidate the molecular pathways that establish them and modulate the level of cellular heterogeneity as a model i will study the mechanism responsible for creating heterogeneity in surface antigen expression in the unicellular parasite trypanosoma brucei antigenic variation is a widely employed strategy by evolutionarily divergent pathogens to evade the host immune response using a multidisciplinary approach i will develop and combine singlecell multiomics lineage tracing and crisprcasbased genome manipulation strategies to characterize the processes pathways and molecules regulating antigen switching in t brucei a better understanding of the mechanisms affecting the level of heterogeneity within a pathogen population will enable us to better predict how pathogens adapt to environmental challenges including those that lead to the emergence of drug resistance in the future this knowledge will enable the development of novel intervention strategies drugs that modulate celltocell heterogeneity to facilitate the clearance of infections
outdoor space selfcalibrating thermoelasticitybased fatigue damage identification

bridge and civil infrastructures maintenance inspection and monitoring are essential to economics and public safety thousand kilometres of road bridges over 100 metres in length in the transeuropean transport network many of the bridges were built during the 1950s and are now nearing the end of their useful life as recent tragical events showed over the last twenty years a large amount of research has been funded by european research framework programmes for investigating materials components and software tools however in horizon 2023 fp the focus has been already pointed to visual inspection techniques with this perspective artemide points to create two fundamental methods to develop an innovative approach for the fullfield identification of fatigue damage in outdoorspace structures based on infrared imaging the first method consists of a selfcalibration and motion compensation tool for image conditioning while the second method allows thermal measurements to be performed consistently and reliably in outdoor environments in this way damage assessment can be performed more efficiently and effectively than is possible today the research will be developed at the university of ljubljana while three months are planned as secondment in dri investment management ltd the largest consulting and engineering company in slovenia in the long term artemide will be fundamental for reaching a relevant academic position in europe with an eye to industrial collaborations with economics and social impacts
beyond the hydrodynamics horizon in the evolution of small and large colliding systems at colliders

a hot expanding environment is produced in heavyion eg leadlead or goldgold collisions in the large hadron collider lhc and relativistic heavy ion collider rhic the produced system expands and cools down turning from a phase with liberated quarks and gluons called quarkgluon plasma qgp to hadrons detectable in the detectors the qgp behaves like a nearly perfect fluid that can be modeled via relativistic hydrodynamics with the smallest observed shear and bulk viscosity over entropy density in the course of the collective expansion the degreesoffreedom interaction develops correlation reflected in the correlation among final hadrons models based on hydrodynamics successfully describe the observed correlations in the experiments observing a similar correlation among final hadrons emitted from much smaller collision systems eg protonproton and protonlead has triggered debates about the nature of the collectivity in such scenarios studies show that the models based on hydrodynamics become less predictive in smaller system collisions in these systems one does not expect a thermalized medium and a framework beyond hydrodynamics is required to explain the true underlying mechanism in collective expansion the main objective of the current project is to prepare a computational tool in the form of an event generator based on the kinetic theory with isotropization time approximation among an extensive list of heavyion collective models this event generator will be unique in explaining small systems that behave particlelike and large systems that act fluidlike in a single framework the model can bridge the experimental measurements and theoretical studies to quantitatively analyze the fluidlikeparticlelike nature of large and small system collisions
safe wheat agriculture towards sustainable health innovative sensing techniques and holistic spectroscopy traceability for improved soil plant health and safe wheat grain

in a world grappling with complex global challenges such as population growth climate change and environmental degradation ensuring security sustainability and food safety is paramount agricultural practices and food production processes are integral to public health economic stability and societal wellbeing however conventional approaches have often operated in isolation limiting our understanding and hindering scalability the wheatwatcher initiative seeks to break these barriers by uniting soil health monitoring plant health assessment and food traceability through a cuttingedge digital soil monitoring system this system assesses soil nutrition chemical and biological factors impacting wheat grains from field growth to flour production spanning multiple european regions by actively involving stakeholders including farmers mill proprietors and policymakers wheatwatcher tailors its solution to practical needs it leverages diverse sensor technologies advanced machine learning models and automated mapping techniques to boost efficiency and scalability a decision support system and cloud platform ensure accessible insights at its core a machine learning model seamlessly integrates technologies creating a cohesive solution that bridges the gaps between soil health plant health and food traceability wheatwatcher aims to foster harmony between sensing technologies data processing and stakeholder engagement revolutionizing comprehensive monitoring
novel lowpressure cryogenic liquid hydrogen storage for aviation

in order to meet the objectives of the european green deal by 2050 in the aviation sector the transition towards h2powered aviation is the solution with the most potential although hydrogenpowered aircrafts exist the current cost of storing and using h2 as a fuel in prolonged flights make their democratization impossible the main blocking point is the absence of viable storage systems of h2 in aircrafts considering the strict limitations in terms of weight volume and costefficiency a sensitivity analysis shows how the economics depend on the tanks gravimetric index gi todays technology can barely achieve 20 gi for 500kg of h2 while industry actors need at the very least 35 gi for 500kg of h2 to transition towards h2powered aviation overleaf intends to develop a game changer liquid hydrogen lh2 storage tank to enable the transition towards h2powered aviation based on a disruptive design under patent process and leveraging innovative materials and technologies the overleaf solution is expected to boast a gi higher than 60 for 500kg of lh2 with no venting over 24h furthermore the concept is an enabler for using the aircrafts fuselage as the outer tank allowing to seamlessly integrate the tank in the aircrafts structureoverleaf will have an interdisciplinary rd approach focusing on advance materials engineering testing and combination at lab and at pilot scale together with appropriate simulation of different design architectures of the hydrogen storage system the project will be based on three distinctive phases and implemented in 7 work packages the consortium includes multidisciplinary partners from 6 different eu countries and contains all the necessary expertise and knowhow to carryout all tasks needed to achieve overleafs ambitious objective
connect2scale uniting european investors and scaleups

connect2scale creates partnerships between five business angel networks estonia finland latvia lithuania and slovenia and two supporting organizations european business angel network and civitta with the aim of enabling the growth of european startups through joint efforts the project focuses on knowledge transfer from venture capital firms to business angels to close the gap between funding cycles and introduce crossborder investment opportunities in the eu in a harmonized way combining unique competences and extensive networks of partners the consortium aims to direct venture capital funding to startups from the moderatemodest ecosystem and to spread the resulting knowledge of the project to a wider circle of investors across the eu
accelerating algae product developments in baltic and north sea

the algaeprobanos project addresses a challenge in the baltic and north sea banos area how to harmonise economic development with social and environmental goals in line with the mission ocean there is a rising demand for green healthy natural consumer and biobased products and algae can become the biomass resource of the future without using fresh water arable land or added fertilisers and or pesticides both the baltic and north sea engulf the necessary conditions to scale up production and produce both commodity and sophisticated algaebased products algaeprobanos apb brings together 26 experts and industry partners from the banos area and beyond to accelerate product development and market access of sustainable algae solutions concretely apb will support six business pilots smes and startups to bring eight algae products to the market trl79 namely in food feed nutraceuticals textiles cosmetics and plant biostimulants applications value chains are based on microalgae and seaweeds sourced in the baltic or north sea or from recycled resources innovation takes place in iterative loops with assessments to secure maximum robustness efficiency and sustainablesustainability furthermore value chains are built with endusers needs put in the centre through engaging users in cocreation activities to ensure maximum uptake digital tools are developed to support all actors from biomass producers to consumers in the end gotomarket strategies are tailored to the pilots providing a trajectory to reach the market in a meta level the algae accelerator develops new and aggregates existing knowledge and develops a portfolio of solutions guidelines and a collaboration and training platform to benefit the entire algae industry and nourish it into a thriving industry by 2050 the legacy of apb will be deposited to the ocean mission banos and the eu4algae
autonomous plasmonenhanced photocatalytic microrobots powered by lorentz force

the 1966 scifi film fantastic voyage portrayed a scientist who miniaturized a submarine to enter his body to remove a blood clot it is only recently that scientists have been able to assemble microrobots from scratch to autonomously move and perform complex tasks such as catching and delivering cargo andor performing chemical reactions the bots use energy from their surroundings or from an external stimulus and turn it into motion lightdriven motion in photocatalytic robots is exceptionally appealing as it allows actuation and control by using an external free energy source ie sun and enhancement of chemical reactions due to two effects selfgenerated micromixing effect and constant surface refreshment giving place to new chemical reactions onthefly yet the reported photocatalytic bots up to date are so slow that their speed can be confused with brownian motion this project seeks to combine two approaches for the first time to enhance the efficiency and speed of lightdriven bots lorentz force as an ultrafast motion mechanism and plasmonic effects for bettering light harvesting a novel system will be introduced in which the robots motion based on the magnetohydrodynamic convection effect is triggered by visible light and can pursue desired reactions degradation of organic wastes and hydrogen generation by leveraging the hosts fundamental photophysical approach in nanoplasmonic design and my interdisciplinary angle on microrobots and energy field the results are expected to bring knowledge gain for the microrobot field and possibly a longterm impact on europes solar technological innovations the projects training comprises transferrable leadership and communication and technical skills development bridging a knowledge gap in photophysics to advance my career as a future group leader in europe with an unorthodox research angle combining photoelectrochemistry and microrobots for alternative energy and environmental solutions
sustainable hydrogen storage by advanced layered magnesiumbased nanostructured alloys

the generation of advanced alloys with extraordinary sustainable functional performance is a game changer for commercializing advanced manufacturing technologies and is a key issue for the 4th industrial revolution considering the environmental issues to reduce co2 emission as well to this end thermally stable highperformance bulk nanostructured nanolayered nanocomposites containing stable interfaces are highly desirable for hydrogen storage within the proposed project the newly developed accumulative foldforging aff method shall be applied to enhance the hydrogen storage response of a mgnb alloy based on extreme grain refinement down to the nanoscale and the synthesis of a nanolayered structure this novel alloy design will assess this synergy between advanced manufacturing by a novel severe plastic deformation spd approach and metal physics as an interdisciplinary topic first the advanced layered system will be designed by aff for nanograins formation and forced alloying between mg and nb then the manufactured new materials shall be characterized in terms of structural features mechanical properties and functional behaviour third atomicscale structural modelling will proceed to simulate sustainable hydrogen storage performance these experiments may give novel insights into tailoring the pathways toward sustainable microstructural design for optimizing the composition and structure of advanced mgnb nanostructured alloys with extraordinary storage capacity and cyclic stability coming from the worldforemost centers on advanced manufacturing and alloy design i will bring new scientific and technological knowledge to the host university and institute meanwhile practical training at one of germanys best universities and research institutes progressing the current stateoftheart by developing metal physics of advanced nanostructured alloys and highquality publications can prepare me for a professorship position in the eu or na
enhanced cybersecurity for networked medical devices through optimisation of guidelines standards risk management and security by design

for the eu health industry to be competitive and to sustainably deliver internationally leading care quality it is important that eu regulation guidelines and standards enable effective and interoperable digital health innovation and promote a vibrant entrepreneurial eu sector safety and competitiveness are not mutually exclusive to deliver on them requires a pace and intensity of technological innovation that is matched by intensive regulatory innovation smarter adaptive dynamic and evidencebased regulatory approaches are needed based on real world experience in representative use scenarios cymedsec has been designed with an optimum consortium of regulatory cybersecurity technology evaluation and clinical eu experts to address exactly this challenge it provides close feedback loops between new technological paradigms and recommendation of regulatory approaches fostering regulatory science fresh thinkingit will deliver novel securitybydesign solutions for the oversight of internet of medical things iomt devices including connected in vitro diagnostics iomt fleet cybersecurity oversight systems will be developed use cases explored include remote patient monitoring and critical care scenarios for which the project will develop novel and highly secure gateway middleware our technological and methodological advancement will go handinhand with detailed review of regulations and guidelines the formal creation of a new iomt cybersecurity standard and evidence collection from representative case studies these objectives are holistically interlinked with learnings form each work area feeding into development and proposals in other areas key to this is the inproject development of a cybersecurity benefitrisk toolbox which will further develop the state of the art using qualitative and quantitively approaches and will make these available as easily usable and findable opensource resources for manufactures and regulatory bodies