DNA sequencer
Comparison

Sequencer
Ion Torrent PGM
454 GS FLX
HiSeq 2000
SOLiDv4
PacBio
Sanger 3730xl
Manufacturer
Ion Torrent (Life Technologies)
454 Life Sciences (Roche)
Illumina
Applied Biosystems (Life Technologies)
Pacific Biosciences
Applied Biosystems (Life Technologies)
Sequencing Chemistry
Ion semiconductor sequencing
Pyrosequencing
Polymerase-based sequence-by-synthesis
Ligation-based sequencing
Phospholinked fluorescent nucleotides
Dideoxy chain termination
Amplification approach
Emulsion PCR
Emulsion PCR
Bridge amplification
Emulsion PCR
Single-molecule; no amplification
PCR
Data output per run
100-200 Mb
0.7 Gb
600 Gb
120 Gb
0.5 - 1.0 Gb
1.9∼84 Kb
Accuracy
99%
99.9%
99.9%
99.94%
88.0% (>99.9999% CCS or HGAP)
99.999%
Time per run
2 hours
24 hours
3–10 days
7–14 days
2–4 hours
20 minutes - 3 hours
Read length
200-400 bp
700 bp
100x100 bp paired end
50x50 bp paired end
14,000 bp (N50)
400-900 bp
Cost per run
$350 USD
$7,000 USD
$6,000 USD (30x human genome)
$4,000 USD
$125–300 USD
$4 USD (single read/reaction)
Cost per Mb
$1.00 USD
$10 USD
$0.07 USD
$0.13 USD
$0.13 - $0.60 USD
$2400 USD
Cost per instrument
$80,000 USD
$500,000 USD
$690,000 USD
$495,000 USD
$695,000 USD
$95,000 USD

Question: What does DNA sequencer include?
Answer: DNA sequencer includes the 454, and SOLiD sequencing platforms.