Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Molecular mechanisms of RBM5-mediated regulation of alternative splicing: impact on cell proliferation and apoptosis. (RBM5)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2010-04-01
End date: 2012-03-31
Duration (months): 24.0
Total project cost (EUR): 152917.0
EU funding (EUR): 152917.0
Participants: 1
Project status: CLOSED

Cancer diseases affect millions of people worldwide and are a source of great burden to society, both financially and in terms of health care resources. In Europe, Every day 5.214 Europeans are diagnosed with cancer and 3.185 die from their disease (20% of total cases are related to lung cancer). Understanding of how genetic factors contribute to these diseases is gathering speed. Genome-wide association analyses on large patient cohorts are generating large sets of candidate genes. A frequent genetic alteration observed in heavy smokers, lung cancer and other tissue carcinomas is the deletion of chromosomal region 3p21.3 where the LUCA15/RBM5 (RNA binding Motif 5) gene maps. RBM5 protein levels are misregulated in several cancers such as lung and breast cancers and constitute one molecular signature associated with metastasis in various solid tumors. RBM5 is an RNA binding protein harboring several domains involved in RNA metabolism and pre-mRNA splicing. Recent data revealed that RBM5 binds to a U/C-rich sequence in the caspase-2 pre-mRNA and that it regulates the alternative splicing of apoptosis-related genes such as the “death receptor” Fas (CD95/APO-1) and its key inhibitor c-FLIP pre-mRNAs. The goal of this proposal is to identify and characterize of RBM5 containing complexes. We will use the Cross Link ImmunoPrecipitation (CLIP) technique coupled to deep sequencing to identify RBM5 RNA targets and to draw a functional map correlating their positions with the alternative splicing event of their targets. To identify RBM5 protein partners, we will use gel chromatography to purify RBM5 containing complex(es) and MALDI-TOFF to identify RBM5 interactors in these complex(es). This information will help us to analyze the regulation of c-FLIP pre-mRNA splicing by RBM5. Hopefully, the results will allow us the design of new potential therapeutic strategies aimed to inhibiting the massive cell transformation occurring during the tumor progression processes.

Keywords: health sciences,lung and breast cancer,rbm5.
The role of Chk1 in hair follicle stem cells and skin tumorigenesis (CHK1 IN STEM CELLS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2010-11-01
End date: 2012-10-31
Duration (months): 24.0
Total project cost (EUR): 173240.8
EU funding (EUR): 173240.8
Participants: 1
Project status: CLOSED

We have found that the ablation of Chk1, a serine-threonine kinase that is activated after DNA damage, strongly reduced the formation of chemically (DMBA/TPA) induced skin tumours, thus suggesting a role of this protein in hair follicle stem cells population, from which tumours are thought to arise. We have observed that deletion of Chk1 causes cell proliferation, DNA damage, and apoptosis within the hair follicle, however the populations affected and the order of events in individual cells have not been clearly defined. I hypothesise that Chk1 deletion causes stem cells to enter an aberrant cell cycle which creates DNA damage during replication and consequently leads to cell death. The purpose of this project is to investigate how Chk1 deletion affects the quiescence, proliferation, and survival of hair follicle stem cells, and to use an in vivo chemical genetic approach to determine whether these processes can be manipulated pharmacologically.

Keywords: chk1,hair follicle stem cells,chemically induced tumours
Solving Aerosol Problems Using Synergistic Strategies (SAPUSS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2010-07-01
End date: 2012-06-30
Duration (months): 24.0
Total project cost (EUR): 154417.0
EU funding (EUR): 154417.0
Participants: 1
Project status: CLOSED

The topic of this research proposal is the source identification of particles in the atmosphere and the physical and chemical transformations occurring in them once emitted. Particles are injected directly into the atmosphere from a number of sources or formed in situ from gaseous precursors compounds. Atmospheric aerosols have several adverse effects on human health and atmospheric visibility, and partly compensate climate forcing by greenhouse gases. These effects depend on particle diameter and chemical composition, as well as on mass and number concentration which exhibits large temporal and spatial variability. Our approach involves measurements of aerosols with multiple techniques occurring simultaneously, to deduce point source characteristics and to understand the atmospheric processes responsible for their modifications. The use of the synergy of highly time-resolved instruments (such as Aerosol Time-of-flight Mass Spectrometer and Aerosol Mass Spectrometer) will help to identify unique sources that would not be possible without multiple highly-resolved instruments working in concert. However, some of these state-of-the-art instruments are not fully characterized yet and they must be coupled with traditional aerosol measurements techniques in order to deduce all the information provided by them. Some of the advantages of these highly time-resolved data occur when they are combined with other data, both high time resolved and time averaged. Additional advantages are obtained when data from an array of instruments are further combined with atmospheric models. The data generated from this proposal will be the input of the receptor-oriented models which will help to re-construct the impacts of emissions from different sources of atmospheric pollutants such as particulate matter. In summary, it is necessary to characterize the physical and chemical properties of atmospheric aerosols and to distinguish between natural and anthropogenic aerosols components.

Keywords: aerosols (climate change),atmospheric chemistry (climate change),human health (global change),atmospheric pollution (climate change)
Measurements and Assessment of desiccation Cracks Integrity in Soils with the Application of Remedial Solutions (RISMAC)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Outgoing Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2009-IOF, 2009)
Start date: 2011-07-11
End date: 2014-07-10
Duration (months): 36.0
Total project cost (EUR): 242927.8
EU funding (EUR): 242927.8
Participants: 1
Project status: CLOSED

The aim of the project is to advance and improve existing non-destructive scanning techniques for detection of desiccation cracks in the soil mass and to develop new remedial measures for cracked soils. The proposal contemplates the validation of geophysical resistivity measurements to detect the presence of desiccation cracks networks in the global soil mass. To date, no experimental technique is capable of performing such studies. Considering that this will be a very valuable tool for industry and other end-users to asses the global integrity of earth-works (such as embankments), it is evident that there is an urgent need to fill this gap. It is also important to bear in mind that the situation will worsen in the near future as the underlying assumption of intact soils (when evaluating their hydraulic conductivity and global stability) becomes even less accurate as a consequence of climate change. Therefore, an experimental technique that is able to quantify the degree of heterogeneities in the soil mass (induce by the presence of desiccation cracks) will be unquestionably necessary. The aim of this project does not stop in detecting the problem but also in proposing novel solutions (based on polymers and grouts) that will contribute to overcome the problems of the current remedial techniques (see previous section) mainly based on the use of lime and cement. In the last few years it has been a considerable advance in the development of new polymers and grouts, which can be very suitable for its application as remedial solutions (and tracers) for cracked soils. Once this project prove the viability of the new remedial solutions (by testing them at different scales and under different environmental conditions, see work plan) it will generate new knowledge that will be ready to transfer it to the industry, benefiting it and the society as a whole.

Keywords: soil mechanics,geotechnics,soil remediation,floods,risk assessment (geophysics),land degradation (climate change)
In-depth quantification and characterisation of PI 3-kinase signalling networks at a System Biology level (PI3K SYSTEMS BIOLOGY)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2011-01-01
End date: 2012-12-31
Duration (months): 24.0
Total project cost (EUR): 172740.8
EU funding (EUR): 172740.8
Participants: 1
Project status: CLOSED

The group of genes termed phosphoinositide 3-kinase (PI3K for short) have roles in many important biological functions and are also implicated in diseases such as diabetes, inflammation, allergy and cancer. Therefore, there are many pharmaceutical and biotechnological companies interested in targeting this pathway. There are 8 different PI3K genes and it is becoming clear that they have different functions and are involved in different diseases. However, although the distinct biological roles of the different PI3K isoforms are being gradually elucidated, there is little information regarding how the different PI3Ks perform their unique biological functions. We will assess using a Systems Biology approach how the different PI3K genes differ in the way by which they affect downstream protein kinases by means of a novel method based on mass spectrometry. This technique is a phosphoproteomic approach that allows quantifying signalling at the system biological level and in an unlimited number of samples and replicates, thus enabling the quantification of protein kinase activation in a comprehensive and robust fashion. Since the technique is not limited by the availability of antibodies, it allows quantifying signalling without a preconception of which pathways may be affected by PI3K; i.e., at the system level. For this, we will inactivate specific isoforms of Class IA PI3K genes (p110α, p110β and p110δ) in cells by pharmacological and genetic means; comprehensive quantification of phosphorylation will then be carried out using quantitative mass spectrometry techniques available in the Host Laboratory. In addition to provide important insights into the biochemical mechanism of isoform specific PI3K signalling from a Systems Biology approach, this work may also lead to the discovery of phosphorylation events that may serve as biomarkers of activity status for specific PI3K isoforms. These biomarkers could be useful for the development of drugs that target these enzymes.

Keywords: proteomics,systems biology,cell signalling,quantitative biology
The functional significance of soluble amyloid beta (Abeta) oligomers for learning and memory deficits in Alzheimer's disease (ABETACOGNITION)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2011-01-01
End date: 2013-11-07
Duration (months): 24.0
Total project cost (EUR): 161661.0
EU funding (EUR): 161661.0
Participants: 1
Project status: CLOSED

Alzheimer’s disease (AD) is characterised by a gradual cognitive decline, which initially affects learning and memory, and gradually impairs other cognitive functions. Despite considerable scientific effort, the cause and pathogenesis of AD is still unknown. Recent in vitro studies suggest that the destruction of synapses by soluble aggregates of amyloid beta (Abeta) might play a role in mediating the progression of the disease. However, what is lacking is an approach that establishes the links between cellular pathology, its causative effect on neuronal network activity in the affected brain areas, and the resulting behavioural impairments. This project aims to address this shortcoming by using a cross-disciplinary design. Core to this project are novel transgenic mice overexpressing soluble (Abeta), which will be tested on object and spatial recognition memory tasks. A novel imaging technique, manganese-enhanced MRI, will be used to establish the effect that soluble (Abeta) has on neuronal activity in the perirhinal cortex and hippocampus, brain areas that are affected early in AD and mediate these types of memory. Lastly, we aim to investigate whether memory deficits in arc(Abeta) mice can be abolished by inhibitors that target elements of (Abeta)-activated signalling cascades recently established in vitro. This will be achieved by direct infusion of drugs into the brain regions of interest in freely behaving arc(Abeta) mice, with the aim of identifying new targets for pharmacotherapeutic intervention. The fellowship at the Max-Planck-Institute for Psychiatry in Munich will enable the applicant, who has received excellent training in the basic field of learning and memory at Oxford and Cambridge, to acquire additional competencies focused on the neuroscience of neuropsychiatric disorders. By moving from the UK to Germany, the fellow will help to circulate scientific knowledge within the European Research Area, towards a common European effort to tackle one of today’s least understood diseases.

Keywords: neuropsychology,brain research,alzheimer's disease,neurodegenerative disease,memory,functional imaging in mice,beta-amyloid,synaptic plasticity,cognition
An existential approach to popular music in Africa: Rock and Metal in Antananarivo (Madagascar) (EXAROMA)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Outgoing Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2009-IOF, 2009)
Start date: 2010-11-01
End date: 2012-10-31
Duration (months): 24.0
Total project cost (EUR): 148470.2
EU funding (EUR): 148470.2
Participants: 1
Project status: CLOSED

Thinking of music in Africa, most still have an image dominated by rather traditional African styles or “Africanized” versions of western sounds. Accounting for the striking role of Rock in a foreign setting, it is the central aim of this anthropological research project to rethink the meaning and relevance of popular music in Africa. Based on the interpretation of ethnographic data on Rock and Metal music in Madagascar’s capital city, the project strives for the establishment of an “existential” approach to popular music in Africa that is clearly based on aesthetic experience. Combining theoretical reflections from anthropology, philosophy, musical aesthetics (both of “high” and of popular music) and the ethnomusicology of Africa, the project aims at substantially contributing to cutting-edge debates not only on popular music in Africa, but also on the role of popular culture in anthropology and, subsequently, on the nature of culture more generally. The scientific debate on popular music and its global circulation is vividly advancing in the USA. Being hosted by the Ethnomusicology Dept. at the Univ. of California, an institution internationally recognized for its outstanding expertise, would therefore provide me with the opportunity to develop substantial scholarship in an upcoming field, still widely neglected in European anthropology. Thus, deeply engaging in sophisticated theoretical discussions during a one-year stay at UCLA would not only give me the unique chance to enhance the interpretation of my ethnographic data. It would also allow for expanding and sharpening my academic profile, perfectly qualifying me for reaching professional maturity and independence. Returning to the Univ. of Bayreuth, known for its high expertise on Africa, I will be able to discuss my findings in a larger regional and historical context, feeding my knowledge into various research contexts and networks, by also essentially contributing to a more complex understanding of Africa.

Keywords: humanities,anthropology,popular music,ethnomusicology,africa,madagascar
BOOKS AND READERS BETWEEN THE IBERIAN AND ITALIAN PENINSULAS TOWARDS AMERICA (SEVILLE AND FLORENCE) (BOOKSREADERSSPIT)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Italy
Call:  (FP7-PEOPLE-2009-IEF, 2009)
Start date: 2010-05-01
End date: 2012-04-30
Duration (months): 24.0
Total project cost (EUR): 214572.4
EU funding (EUR): 214572.4
Participants: 1
Project status: CLOSED

The first aim of my project is to study the development of a new kind of readers in Europe in the Early Modern period, those who were interested in works of humanities (understood as the erudition and fine writing), and the influence of cultural transferences on it. Until this moment, these humanities works have been studied from the perspective of its lenguage, its authors or its bibliographical characteristics, but the questions proposed in this project are different. An interdisciplinary perspective is compulsory, a perspective in which economic, social and cultural history are combined; because the objetive is not only to identify those who were their readers, but also to know how they assimilated and responsed to these texts. These readers seem to have been members of the urban oligarchy. The starting point for this project will be the Seville readers living during the Spanish Golden-Age (XVIth-XVIIth centuries), this study should be done overcoming the local view with a global perspective of analysis. In that sense, the close cultural relation between Spain and Italy in the Early Modern period, leads us to study the role that the emulation of Italy played in the formation of “cultured” Spanish readers. This offers us the possibility to evaluate the role played by the cultural exchange with Italy in the appearance and development of this type of readings, as well as compare Spanish and Italian libraries. This research will allow us to evaluate the degree in which the Spanish colony in Italy served as a bridge or road for the transmission of works of humanities to the Iberian Peninsula, and from there to the Americas. Therefore, the main objetive of my project is to study the cultural tranference in Europe (and the Americas) through the mechanisms of the circulation of books and texts. Those mechanims can be professional (booksellers) or non-professional (I will pay speciall attention, for example, to the correspondence between erudites).
The post-copulatory interplay between females and ejaculates in social insects. (CONFLICTS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Outgoing Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Denmark
Call:  (FP7-PEOPLE-2009-IOF, 2009)
Start date: 2011-03-01
End date: 2015-05-06
Duration (months): 36.0
Total project cost (EUR): 271667.3
EU funding (EUR): 271667.3
Participants: 1
Project status: CLOSED

The hymenopteran eusocial insects (ants, bees, wasps) not only have impressive colony lives, but also spectacular adaptations for the years-long storage of live sperm. Where human medicine requires cryotechnology to do this, the queens of honeybees and ants produce glandular secretions to maintain sperm at ambient temperatures inside a specialized storage organ (spermatheca). During my PhD, I pioneered the study of male accessory glands and their effects on sperm viability during insemination, using mostly leafcutter ants. I discovered that ejaculate competition has evolved in ants and bees with multiply mated queens, but is terminated by ant queens after sperm storage. This made it clear that female secretions play key roles and that proteomic approaches are needed to understand their function. I plan to use the honeybee Apis mellifera to study the effects of female glandular secretions in situations of potential sexual conflict. These arise when queens are inseminated by multiple males and have an interest in selecting the most viable sperm fraction for permanent storage and later use when fertilizing eggs. I will combine evolutionary biology with proteomics technology to characterize and identify the proteome of secretions that newly mated queens add to ejaculates while sperm is transferred, maintained in the reproductive tract for up to several days, and finally stored for years. I will also study the specific effects of oviduct- and spermatheca secretions on ejaculate physiology and sperm viability. Finally, I will combine biochemical and experimental approaches to clarify the function of specific protein components within these female secretions. My research will provide novel answers to general evolutionary questions about the expression and regulation of sexual conflict. It will develop novel technology with considerable potential for general studies of sperm viability and for applied honeybee breeding studies at times when many commercial stocks are declini

Keywords: sexual selection,proteomics,social insects,cryptic female choice,honeybees,biochemistry