Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
MVISION (MVISION)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Co-funding of regional, national and international programmes
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2011-COFUND, 2011)
Start date: 2012-06-15
End date: 2018-06-14
Duration (months): 72.0
Total project cost (EUR): 6701820.0
EU funding (EUR): 2680728.0
Participants: 1
Project status: CLOSED

MVision programme intends on building a particularly favourable environment to train and reinforce career development of best researchers in biomedical imaging. Foundation Madri+d (a public foundation dependent on the Madrid Regional Government) will count with the support of the Massachusetts Institute of Technology to catalyze fellowships biomedical imaging education and research. The objectives of the project are: (i) leveraging Madrid’s investment in biomedical imaging infrastructure and clinical network and MIT’s experience in integrating biomedical imaging research and education (ii) attracting and nurturing top talents by providing broad-ranging education and research experiences in cutting edge biomedical areas (iii) promoting work that is translational and posed for impact in medicine and healthcare (iv) creating a network of professionals in the academic, medical, industry and government sectors. MVision in its 60 months length will offer 30 fellowships to experienced researchers under adequate researcher labour conditions to develop research projects in the frame of hosting institutions (universities, research centres, pharmacies, chemistries or hospitals). MVision offers the opportunity to receive top level customized training in Madrid and Boston, helping fellows to design their research projects during the first year and providing them two to three years to implement their individual driven research projects in a wide range of hosting institutions. Fellows will benefit from the knowledge, expertise, and existing networks of hosts and MIT, and will be encouraged to foster long term relationships. Researchers will be selected by an international panel through a transparent evaluation process following the European Code of Conduct for the recruitment of researches and the Charter for Researchers. The regional government of Madrid believes MVision Programme is a long-term investment in order to become a world known centre for biomedical imaging.

Keywords: biomedical imaging,madrid,mit,fellowship,molecular medicine,diagnostics,visualisation,interventions,cellular biology,physiology,genetics,physics,chemistry,biomedical engineering
ERA-NET for Coordinating Action in Plant Sciences (ERA-CAPS) (ERACAPS)

Framework: FP7 — Cooperation
Programme: Food, Agriculture and Fisheries, and Biotechnology
Destination: Sustainable production and management of biological resources from land, forest, and aquatic environment
Action type: Coordination and support actions
Partnership: ERACAPS (ERA-NET)
Countries involved: Austria,Belgium,Canada,Denmark,Estonia,France,Germany,Hungary,Israel,Italy,Latvia,Netherlands,Norway,Poland,Portugal,Serbia,Spain,Hungary,United Kingdom,New Zealand
Call:  (FP7-ERANET-2011-RTD, 2011)
Start date: 2011-12-01
End date: 2015-05-31
Duration (months): 42.0
Total project cost (EUR): 2220093.63
EU funding (EUR): 1989658.0
Participants: 23
Project status: CLOSED

Plant sciences face important challenges at the European and global scale due to a burgeoning world population that requires sustenance. Reliable production of high-quality and safe food, feed and renewable Carbon supplies for green chemistry, without the use of excess land, energy, water, pesticides and chemicals is therefore essential. To ensure that we have the scientific understanding to again revolutionise agricultural capabilities to deliver higher yields with lower inputs in a changing climate, we propose a network focusing on Coordinating Action in Plant Sciences (ERA-CAPS). This will unite the scientific and economic capabilities of member states and enable the coordination of sustainable transnational plant science research programmes. ERA-PG (2004-2009) successfully initiated a programme to structure the scientific and technological basis for plant genomics programmes in Europe. This has fostered the development of the common knowledge base necessary to build coherent transnational policy frameworks. However, if transnational cooperation and resolute mutual goals in the plant sciences are to be firmly embedded in national policies and processes, these foundations must be strengthened and expanded. To bring this about ERA-CAPS will pursue the development of a common agenda for plant science in Europe and create a joint research programme. ERA-CAPS will also facilitate data management, access and sharing solutions. Through external engagement ERA-CAPS will enable interaction between researchers, funders and relevant European initiatives, and assist in the systematic exchange of information to facilitate the expansion of the network and the involvement of new members and affiliates. Such a network will significantly help plant sciences address both current and future challenges in food and non-food crop production.

Keywords: plant sciences,genomics,agriculture,crops,food security,sustainable production,coordination
Native Dynamic Peptides: application to the preparation of self-replicating peptides and dynamic proteins using dynamic combinatorial chemistry (NADYPE)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-12-01
End date: 2013-11-30
Duration (months): 24.0
Total project cost (EUR): 50000.0
EU funding (EUR): 50000.0
Participants: 1
Project status: CLOSED

A Dynamic combinatorial library (DCL) is formed by the interconnection of building blocks by reversible covalent or non-covalent bonds. In such DCL, all possible combinations of the initial constituents may form, in proportions specific to the thermodynamic equilibrium of the system. Disruption of this equilibrium by physical or chemical factors, may lead to adaptation through amplification of one or more constituents of the library by rearrangement of its components. This dynamic combinatorial chemistry (DCC) approach has been successfully used in the 15 past years, in particular for the discovery of drugs selected by the biological target itself by in situ molecular recognition. In this project, we want to implement the DCC concept towards native peptide chemistry. Our main objective is to create a new class of constitutionally dynamic peptide that would combine the structural, catalytic, and (bio)recognition properties of peptides, or proteins, with the adaptive character of constitutionally dynamic assemblies. The objectives of the present research project are threefold. The first is to pioneer a firmly original chemical methodology to make some peptidic bond reversible under physiological conditions. The second is to probe the applicability of this methodology for the preparation of self-replicating dynamic coiled-coil peptides. The third is to prepare a dynamic analog of streptavidin from a dynamic combinatorial library of small peptides. Such an achievement will open the door to the development of dynamic proteins that could adjust their composition and structure in response to a binding event. For instance, this approach may in principle allow the “self-fabrication” of dynamic enzymes, receptors or antibodies able to adapt their composition, in a chemically controlled evolution process, to fit their target (a substrate, a receptor on a cancer cell, or a pathogenic protein).

Keywords: physical sciences,dynamic combinatorial chemistry,peptides,self-assembly,self-replication
Tailoring Enzymes for Natural Product Synthesis (NPTAILORINGENZYMES)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-10-01
End date: 2015-09-30
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

The aim of the project is to translate the knowledge from biosynthetic pathways of natural products into Organic Chemistry, achieved by developing novel chemoenzymatic total syntheses of the polyketide natural products ambruticin and jerangolid. In both biosynthetic pathways, large parts of the molecular complexity are set up by tailoring enzymes. These enzymes will be studied regarding their potential to act as biocatalysts that can substitute late and crucial steps in total syntheses. The project divides into two parts. At first, the biosynthesis of ambruticin and jerangolid will be studied with a focus on allocating particular steps to individual tailoring enzymes. These enzymes will be characterised in in vitro assays and these assays will be optimised in order to find out if the candidate enzymes can be applied as efficient biocatalysts. After this, total syntheses will be developed, which integrate these enzymatic steps. This approach promises to gain several advantages. The overall efficiency of the syntheses could be drastically improved and the access to derivatives could also be considerably facilitated. Furthermore, the project will gain substantial knowledge about tailoring processes of complex polyketide pathways.

Keywords: organic chemistry,homogeneous catalysis,biocatalysis,chemoenzymatic,natural product chemistry,catalysis,biosynthesis
Conformational dynamics of single molecules under force (FORCEPROT)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-09-01
End date: 2015-08-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

The long term aim of this proposal is to decipher the molecular mechanisms by which proteins equilibrate under the effect of a constant stretching force. We will use the newly developed single molecule force-clamp spectroscopy technique to elucidate, with exquisite sub-Ǻngström sensitivity, the dynamics of proteins as they unfold, collapse and refold in response to a mechanical force. We will first examine the conformational dynamics of a single refolding protein during its individual folding trajectory from highly extended states. In particular, we will focus on the characterization of the newly discovered ensemble of collapsed states that hold the key to explaining how an extended polypeptide folds while regaining its mechanical stability. We will expand this novel methodology to study the collapsed conformations of the amyloid forming proteins α-synuclein, which causes Parkinson’s disease, Aβ42, responsible for Alzheimer’s disease, and γD-crystallin, which triggers the cataracts in the eye lens. While current studies have mainly focused on the mechanisms of bulk aggregation and amyloid formation, an emerging consensus is that conformational diseases originate at the single molecule level, when an innocuous monomer undergoes a structural transition into a toxic β-sheet conformation. The uncanny ability of single molecule techniques to observe the acquisition of rare misfolded conformations will help establish mechanistic paradigms for developing a unified molecular scale understanding of the origins of these diseases. Finally, we will use our force-clamp assay to examine how force affects the chemical mechanisms of disulfide bond reduction in proteins exposed to mechanical forces. Within a multidisciplinary approach, here we propose a series of innovative experiments to directly probe the effect of force on the function of an individual folding polypeptide and the mechanisms by which mechanical forces modulate chemical reactions, of common occurrence in nature

Keywords: single molecule force-clamp spectroscopy,protein folding,conformational diseases,biophysics,chemistry under force,conformational dynamics
Towards construction of a comprehensive map of amyloid-ligand interactions: (-)-Epigallocatechin 3-Gallate and insulin amyloid (EGCG+INSULIN=)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Lithuania
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-08-01
End date: 2015-07-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Increasing number of peptides and proteins are found to form ordered fibrillar aggregates under certain conditions. Amyloid-like fibrils are associated with a number of diseases, ranging from neurodegenerative diseases to systemic amyloidoses. Current studies have shown the ability of (-)-Epigallocatechin 3-Gallate (EGCG) to inhibit fibril formation and even to dissociate fibrils. Nevertheless, some EGCG-resistant structures were also reported. To evaluate EGCG as a possible drug candidate against amyloid diseases, extensive studies of protein aggregation in presence of the ligand is necessary. The proposed research will construct a comprehensive map of EGCG-insulin amyloid interactions. 1. At varying conditions insulin forms fibrils via different pathways. The impact of EGCG on the pathway of aggregation will be studied. 2. At varying conditions insulin aggregates into distinct amyloid structures, mimicking prion-like ‘strains’. The impact of EGCG on insulin ‘strains’ will be studied. 3. Insulin fibrils can elongate at conditions not favorable for de novo fibril formation. The impact of EGCG on fibril elongation (mimicking prion-like infectivity) will be studied. Isothermal titration calorimetry (ITC) will be used to study insulin-EGCG binding. Also ITC will be used to determine thermodynamic parameters of fibril elongation. Differential scanning and pressure perturbation calorimetry (DSC and PPC), ultrasound velocimetry, densitometry and fluorescence spectroscopy will give insight into kinetics and thermodynamics of insulin aggregation in presence of EGCG at different conditions. Fourier-transform infrared (FTIR) spectroscopy will be used to monitor changes in secondary structure, and atomic force microscopy (AFM) will show the morphology of aggregates.

Keywords: amyloid-ligand interactions,insulin,(-)-epigallocatechin 3-gallate,thermodynamic analysis,amyloidogenic diseases,drug candidate
Emergence of tissue mechanical properties from molecular and cellular activity during morphogenesis. (MORPHOMECHANICS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-09-01
End date: 2015-02-28
Duration (months): 42.0
Total project cost (EUR): 87500.0
EU funding (EUR): 87500.0
Participants: 1
Project status: CLOSED

Morphogenesis refers to the set of complex movements and deformations that tissues experience during development to give rise to the three dimensional form of organs and organisms. From a biomechanical perspective tissues represent a unique class of materials, which exhibit dynamic mechanical properties that evolve over time. Little is known about how these macroscopic properties arise from the interplay and integration of molecular, cellular and tissue events. In this project, I propose to use the process of Dorsal Closure of the Drosophila embryo to undertake a dynamic study of morphogenesis spanning the molecular, cellular and tissue levels, to obtain insights into how tissue properties emerge from the coordinated activity of its constituent cells. These studies will focus on the amnioserosa, one of the main force-generating tissues during DC that has proved to be a powerful system to study the emergence of morphogenetic forces. The focus of this project is to understand the molecular mechanisms underlying actomyosin activity, how they translate into effective cell shape changes and how this activity is coordinated across the whole tissue to give rise to the contractile activity of the tissue. For this, I will use a combination of classical genetics and state of the art microscope techniques to obtain quantitative information on the dynamic behaviour of molecules, sub-cellular modules and cells. I will investigate how molecular and cellular events impinge on the mechanical properties of the tissue, which will be probed by laser ablation experiments in wild type and in embryos with perturbed actomyosin dynamics. The multi-disciplinary research outlined in this project has the potential to make significant contributions to the young field of cell-developmental biology and the physical and engineering sciences.

Keywords: morphogenesis,contractility,stiffness,actomyosin dynamics,adhesion
Ecosystem-based management strategies for urban wastewater systems (ECOMAWAT)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-09-01
End date: 2015-10-12
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Ecosystem-based management strategies for the urban wastewater system (UWWS) are needed for the successful implementation of Water Framework Directive. UWWS discharges should be dynamically regulated to not exceed the river waste treatment capacity. The overall aim of this project is to increase the knowledge about the interactions between UWWS and rivers in order to propose strategies to maximize ecosystem services at the same time that operating costs of the UWWS are minimized. Field experimental work will be conducted to understand the effects of UWWS discharges (organic matter, nutrients and priority pollutants) and river waste treatment capacity. A dynamic model of the whole UWWS and river will be developed and several management strategies will be tested against different types of environmental conditions and disturbances. These strategies include implementation of control and infrastructure upgrades. The knowledge acquired will be integrated into an Environmental Decision Support System that water managers can use to make decisions.

Keywords: technological sciences,natural sciences
Impact of food ingredients and contaminants on endogenous inflammation mediators – Targeted metabolomics of the eicosanoid pathway (FOOD-OXYLIPIN-TOX)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-09-01
End date: 2015-08-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Eicosanoids and oxidative metabolites of other poly-unsaturated fatty acids represent one of the most potent classes of endogenous cellular mediators. Although about 80% of pharmaceuticals influence the formation of these oxylipins, little is known in regard to alteration by food ingredients. The aim of this project is to investigate the effects of these compounds on endogenous oxylipin levels applying a targeted metabolomics approach. Numerous studies on the impact of nutrition on single eicosanoids have been carried out. Because biological effects arise from a multitude of oxylipins, this approach is incomplete. To solve this problem, we will simultaneously analyze the whole oxylipin pattern of all unsaturated fatty acids using liquid chromatography mass spectrometric methods (LC-MS). This metabolomics approach will provide a more complete description of the biological effects of food ingredients. In a complementary approach, we will test the in vitro effect of food ingredients on the enzymes involved in the pathways of oxylipin formation, particularly their inhibition/activation, as well as their expression levels. For this purpose cell-free enzyme assays for the cyclooxgenase, lipoxygenase, cytochrome P450 and epoxide hydrolase pathways will be established. Pro- and anti-inflammatory effects of the compounds will be investigated in primary cell cultures cells followed by LC-MS read out. With these high-content screening tools, secondary plant metabolites contaminants and food additives will be investigated for modulation of oxylipin formation. The most active compounds will be tested in animal models for effects on regulatory lipids and on physiological end points including atherosclerosis, inflammation and pain. It is proposed that these studies will lead to the discovery of food ingredients that alter regulatory lipids, provide understanding of the system involved, while providing new tools for the pharmacological and toxicological evaluation of xenobiotics.

Keywords: instrumental analysis,food products,toxicology,metabolism,nutrition related disorders,dietetics
Epigenetic modifications in glomerular nephropathy and renal aging (RENAL EPIGENETICS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Life-long training and career development
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2011-CIG, 2011)
Start date: 2011-09-01
End date: 2018-07-12
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Here, we propose a program to identify the role of mTOR and autophagy dependent epigenetic modifications during kidney aging. We have assembled a unique set of complementary mouse models including kidney specific and timely inducible autophagy and mTOR deficient mice. For the detailed molecular analysis of transgenic podocyte tissue, we have generated a double-labeled transgenic mouse model, where podocytes expressing green fluorescent protein are contrasted by other kidney cells expressing red fluorescent protein. Using this mouse model, we have pioneered a cell sorter based purification of glomerular podocytes from kidney single cell suspensions, which allows us to perform the first epigenetic analysis of aged and transgenically modified podocytes. mTOR and / or autophagy dependent gene expression signatures, histone modifications and chromatin-remodeling patterns associated with kidney aging in transgenic and wildtype mice will be identified. We recently showed that mTOR hyperactivation at early stages of diabetic nephropathy determines the progressive course of the disease. We now hypothesize that transient mTOR hyperactivation may induce epigenetic modifications representing the clinically observed, but still unresolved “metabolic memory” of diabetic nephropathy. In summary, this project aims to elucidate fundamental biological mechanisms of kidney aging. This may ultimately aid in risk prediction and improved targeted medical interventions to promote healthy aging and to prevent chronic kidney diseases such as diabetic nephropathy.

Keywords: health sciences,nephrology,epigenetic,kidney,glomerulus,podocyte,aging,diabetic nephropathy,chronic kidney didease,dna-methylation,mtor,autophagy