Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Single molecule characterisation of biological nanopores in action (NOTE)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Netherlands
Call: Individual Fellowships (H2020-MSCA-IF-2020, 2020)
Start date: 2021-10-01
End date: 2023-09-30
Duration (months): 24.0
Total project cost (EUR): 175572.48
EU funding (EUR): 175572.48
Participants: 1
Project status: SIGNED

Biological nanopores are nanometre-scale holes in membranes created by transmembrane proteins. In nature, nanopores come from pore-forming toxins or exist as transporters in bacteria. To date, they have been successfully used for an impressive variety of applications ranging from DNA sequencing through to the measurement of function-related motions of enzymes. Planned developments using nanopores include their integration into biosensors for the detection of analytes from blood. However, to reach their potential, miniaturisation and parallelisation of electrophysiology setups and an in-depth understanding of how nanopores exert forces on their substrates during trapping and translocation are required. There are currently no experimental techniques which provide access to this information. Single molecule studies using the optical tweezers (OT) have, over the past decade, become the gold standard in high resolution measurements of forces in biological systems, including observations of sub-nanometre enzyme kinetics, protein folding and protein degradation machinery in action. I propose the development of low-cost, microfluidic-based tools for the study of biological nanopores using OT. The technologies arising from this work will provide an unprecedented insight into the function of biological nanopores and will enable studies of any other transmembrane protein system, many of which are also of great medical importance. The findings will also lay the groundwork for peptide-sequencing and biosensor technologies for personalised medicine. The combination of my skills in single molecule biophysics and Prof. Maglia’s expertise at the forefront of biological nanopore technologies is ideal for the successful completion of the research objectives. Through the planned interdisciplinary work and high-quality training, I will increase both my research- and transferable skill sets enabling me to reach my goal of establishing a leading independent research group within the EU.

Keywords: single molecule,optical tweezers,biological nanopores,electrophysiology,microfluidics,personalised medicine
Enhancing the EU's Transboundary Crisis Management Capacities: Strategies for Multi-Level Leadership (TransCrisis)

Framework: Horizon 2020 — Societal Challenges
Programme: Europe in a changing world - inclusive, innovative and reflective societies
Action type: Research and Innovation actions
Countries involved: Spain,Italy,Hungary,Sweden,Netherlands,Netherlands,Denmark,United Kingdom
Call: Political challenges for Europe (H2020-EURO-SOCIETY-2014, 2014)
Start date: 2015-04-01
End date: 2018-03-31
Duration (months): 36.0
Total project cost (EUR): 2280809.5
EU funding (EUR): 2280209.0
Participants: 8
Project status: CLOSED

The European Union (EU) is facing the daunting prospect of transboundary crises: threats that escalate across national borders and policy domains. EU member states must collaborate to address these crises. EU governance can play a pivotal role in facilitating a joint response. But does the EU have the institutional leadership capacities to deal with transboundary crises? The response to the financial crisis – a textbook example of a transboundary crisis – revealed deep problems with crisis leadership, including conflicting diagnoses, regulatory gaps, unclear political jurisdictions and responsibilities, a lack of problem solving capacity, and blame-shifting. Growing euroscepticism has been directly related to the EU’s role during this transboundary crisis. This project outlines the institutional requirements for effective and legitimate crisis leadership in the face of transboundary crisis. We define crisis leadership as a set of strategic management functions, including the detection of impending threats, the collection and sharing of information, the coordination of partners, and the communication to the public about the crisis and the response. The project analyses the capacities of political leaders in EU institutions and member states to fulfill these leadership functions. It will pinpoint the existing and required capacities to support these functions. It investigates the crisis management capacities of individual political leaders, and EU institutions. It explores the effects of political leadership on the member state level and studies how crisis management capacity is exercised in various policy sectors. The project will result in recommendations for effective and legitimate crisis leadership. It establishes a ‘crisis management capital index’ that allows for an evidence-based assessment. It proposes strategies to build support for transboundary crisis management in a multilevel system, reconnecting citizens with an idea of what the EU can do for them.

Keywords: political leadership,crisis management,transboundary crisis,multi level governance
Optopharmacological brain mapping of autism mouse models (OPTOFRAX)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Reintegration panel
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Spain
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2014, 2014)
Start date: 2015-07-01
End date: 2017-06-30
Duration (months): 24.0
Total project cost (EUR): 170121.6
EU funding (EUR): 170121.6
Participants: 1
Project status: CLOSED

Manipulation of neurons with light-regulated proteins and drugs has opened unprecedented possibilities for phototherapies for neurological diseases. The host laboratory has developed a powerful new optopharmacological tool: a selective photoswitchable modulator of metabotropic glutamate receptors (mGluR). This drug can be activated or inactivated with light so that its action can be finely patterned in space and time. We aim at developing a novel application for this optical tool: a brain functional mapping technique. We will test it at two different levels of resolution. First, we will map the therapeutic site of action at whole-brain level. Inhibition of mGluR is a promising therapeutic strategy for autism disorders, including Fragile X, but the brain region responsible for this effect is not known. We will inactivate the drug locally by illuminating specific regions of the brain and will identify which region is the target for the therapeutic effect. Second, we will map the physiological contribution of mGluR to neuronal plasticity at the minimal spatial resolution: the individual synapse. Activation of mGluR induces long-term synaptic depression at the level of neurons, but it is unknown if it is also true at the level of single synapses. We will inactivate the drug in a single synapse using laser light and will test whether this specific synapse undergoes structural depression. This project will prove the utility of optopharmacological tools to map the therapeutic and phyisological mGluR function. This original approach could open countless possibilities for scientific, medical, and commercial applications. The project is an excellent opportunity for the Researcher to further develop his knowledge and to position him in unique multidisciplinary environment that will foster his career progression towards independence. At the same time, the Researcher’s experience acquired at MIT will be extremely valuable for the host institution and the European Research Area.

Keywords: optopharmacology,fragile x,phototherapy,brain mapping,metabotropc glutamate receptors,long term depression,synaptic plasticity
Coordinating Research and Evidence for Medical Devices (CORE-MD)

Framework: Horizon 2020 — Societal Challenges
Programme: Health, demographic change and wellbeing
Theme: Methods and data
Action type: Coordination and support actions
Countries involved: Austria,Spain,Ireland,Italy,Austria,Spain,Italy,Belgium,Belgium,Belgium,Belgium,Sweden,Switzerland,Poland,Sweden,Switzerland,France,Netherlands,Netherlands,United Kingdom,Denmark
Call: Developing methodological approaches for improved clinical investigation and evaluation of high-risk medical devices (H2020-SC1-2020-Single-Stage-RTD, 2020)
Start date: 2021-04-01
End date: 2024-03-31
Duration (months): 36.0
Total project cost (EUR): 2360977.5
EU funding (EUR): 2360977.5
Participants: 23
Project status: SIGNED

CORE–MD will translate expert scientific and clinical evidence on study designs for evaluating high-risk medical devices into advice for EU regulators, to achieve an appropriate balance between innovation, safety, and effectiveness. A unique collaboration between medical associations, regulatory agencies, notified bodies, academic institutions, patients’ groups, and health technology assessment agencies, will systematically review methodologies for the clinical investigation of high-risk medical devices (Work Package 1), recommend how new trial designs can contribute (Work Package 2), and advise on methods for aggregating real-world data from medical device registries with experience from clinical practice (Work Package 3). Multidisciplinary workshops will propose a hierarchy of levels of evidence from clinical investigations; educational and training objectives for all stakeholders, to build expertise in regulatory science in Europe; and an ethics charter for medical device innovation (Work Package 4). Industry participation will be invited. Specific CORE–MD tasks will advise on optimal statistical methods, the utility of patient-reported outcomes, the conduct of registry trials, clinical criteria for evaluating artificial intelligence as a medical device, and how to evaluate medical devices used in children. The essential principles of medical device trials will be considered jointly with the Good Clinical Trials Collaborative. Links between CORE–MD partners will catalyse sustainable networks for research. The consortium is led by the European Society of Cardiology and the European Federation of National Associations of Orthopaedics and Traumatology, and involves all 33 specialist medical associations that are members of the Biomedical Alliance in Europe. Final recommendations will be submitted to the Working Group on Clinical Investigation and Evaluation of the European Commission to be considered when developing EU guidance or common specifications.

Keywords: high risk medical devices,regulatory science,evidence based medicine,trial methodologies,clinical investigations,device registries,medical device regulation,cardiology,orthopaedics,diabetes
Cobalt-catalyzed late-stage functionalization of arenes through metal-ligand cooperation (CobaltLSF)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Global Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Chemistry
Countries involved: Germany,United States
Call: Individual Fellowships (H2020-MSCA-IF-2020, 2020)
Start date: 2021-05-01
End date: 2024-04-30
Duration (months): 36.0
Total project cost (EUR): 246669.12
EU funding (EUR): 246669.12
Participants: 2
Project status: SIGNED

The depleting reserves of precious metals within the Earth’s crust has made their replacement with more abundant first-row transition metals of paramount importance. Pincer-ligated cobalt complexes have shown a unique ability to recapitulate the reactivity of commonly employed iridium catalysts for arene C–H borylation but, notably, with complementary ortho-to-fluorine site selectivity. However, the lower activity and functional group tolerance of cobalt catalysts has precluded their use in more demanding late-stage applications, depriving chemists of the possibility to selectively functionalize fluoroarenes within complex molecules. “CobaltLSF” aims to unlock the full potential of Co-catalyzed arene C–H functionalization with the use of a novel carbene-containing NCN-pincer ligand that is designed to promote metal-ligand cooperation to address three main challenges: (1) chemoselectivity, by facilitating key steps such as C–H bond cleavage and bond-forming reductive elimination, improving the efficiency of desired catalytic processes over competing side-reactions; (2) site selectivity, through ligand-assisted C–H activation which is predicted to result in a rapidly reversible process that would reinforce ortho-to-fluorine selectivity; (3) direct C–H alkylation using olefin coupling partners, with the aim of expanding the bond-forming capabilities of cobalt catalysis beyond C–H borylation in the context of a green, waste-free method for installing alkyl chains. Alongside the development of (NCN)Co catalysts, their application to the late-stage functionalization (LSF) of fluoroarene-containing drugs will be examined and their chemo- and site selectivity benchmarked against known LSF methods, providing useful comparative data for end-users, such as medicinal chemists. Overall, the successful realization of the proposal is expected to have far-reaching implications for the use of carbene-containing pincer ligands in C–H functionalization with earth-abundant metals.

Keywords: cobalt,catalysis,pincer,c h functionalization,borylation,alkylation,late stage
Charting a New Horizon of Big and Fast Data Analysis through Integrated Algorithm Design (BigFastData)

Framework: Horizon 2020 — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Consolidator grants
Action type: ERC actions
Panel: Computer science and informatics
Panel description: Informatics and information systems, computer science, scientific computing, intelligent systems
Countries involved: France
Call: ERC Consolidator Grant (ERC-2016-COG, 2016)
Start date: 2017-09-01
End date: 2023-12-31
Duration (months): 76.0
Total project cost (EUR): 2472752.0
EU funding (EUR): 2472752.0
Participants: 1
Project status: SIGNED

This proposal addresses a pressing need from emerging big data applications such as genomics and data center monitoring: besides the scale of processing, big data systems must also enable perpetual, low-latency processing for a broad set of analytical tasks, referred to as big and fast data analysis. Today’s technology falls severely short for such needs due to the lack of support of complex analytics with scale, low latency, and strong guarantees of user performance requirements. To bridge the gap, this proposal tackles a grand challenge: “How do we design an algorithmic foundation that enables the development of all necessary pillars of big and fast data analysis?” This proposal considers three pillars: 1) Parallelism: There is a fundamental tension between data parallelism (for scale) and pipeline parallelism (for low latency). We propose new approaches based on intelligent use of memory and workload properties to integrate both forms of parallelism. 2) Analytics: The literature lacks a large body of algorithms for critical order-related analytics to be run under data and pipeline parallelism. We propose new algorithmic frameworks to enable such analytics. 3) Optimization: To run analytics, today's big data systems are best effort only. We transform such systems into a principled optimization framework that suits the new characteristics of big data infrastructure and adapts to meet user performance requirements. The scale and complexity of the proposed algorithm design makes this project high-risk, at the same time, high-gain: it will lay a solid foundation for big and fast data analysis, enabling a new integrated parallel processing paradigm, algorithms for critical order-related analytics, and a principled optimizer with strong performance guarantees. It will also broadly enable accelerated information discovery in emerging domains such as genomics, as well as economic benefits of early, well-informed decisions and reduced user payments.

Keywords: big data analytics,data management systems,parallel data processing,data stream processing,query optimization
Green CURe In Overheated CITY spaces: An investigation of childhood heat-related health impacts and protective effects of urban natural environments. (Green CURIOCITY)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Reintegration panel
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Spain
Call: Individual Fellowships (H2020-MSCA-IF-2019, 2019)
Start date: 2020-09-01
End date: 2022-08-31
Duration (months): 24.0
Total project cost (EUR): 172932.48
EU funding (EUR): 172932.48
Participants: 1
Project status: CLOSED

Green CURIOCITY: As a consequence of climate change, the European climate is getting warmer and the impact on childhood health and development is insufficiently understood. Equally, the potential to reduce heat related health risks through nature based solutions (NBS), such as exposure to urban natural environments (for example urban green spaces), calls for further investigation. Green CURIOCITY aims to provide evidence for improved knowledge about how heat exposure during pregnancy affects birth outcomes and how long-term exposure to heat may influence children’s neurodevelopment. The project will also investigate how adverse effects can be prevented through a potentially mitigating impact of urban natural environments in the context of NBS. Green CURIOCITY has a strong impact and implementation component, both by proposing to develop vulnerability maps, demonstrating urban “hot-spots” where the risk of negative impacts of heat is aggravated due to specific socio-demographic and land use patterns and by the development of a plugin tool for a Geographic Information System software to predict impact of urban natural environments on childhood heat-related health outcomes in partnership with WHO. The suggested methods include advanced statistical and geospatial analyses in combination with map production and software development. We will use existing data from the Human Early-Life Exposure (HELIX) cohort and link to state-of-the-art environmental exposure data to assess impact on birth and neurodevelopmental outcomes. The results are expected to be of high relevance and impact due to the project’s alignment with contemporary urgent challenges related to climate change, urban landscapes, and the health of future generations. In particular, the project’s focus on solutions and communication tools will provide opportunities for innovative evidence-based implementation strategies.

Keywords: heat exposure,birth outcomes,neurodevelopment,childhood health,climate change,nature based solutions,urban green spaces,urban natural environments,urban planning,mapping,policy impact
Control of the Homeostasis of Actin through Time: actin homeostasis during embryonic development by means of single-molecule imaging and simulation in C. elegans (CHAT)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Reintegration panel
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: France
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2014, 2014)
Start date: 2015-04-01
End date: 2017-03-31
Duration (months): 24.0
Total project cost (EUR): 185076.0
EU funding (EUR): 185076.0
Participants: 2
Project status: CLOSED

In embryonic cells, actomyosin forms a cortical reticulated gel that turns over rapidly, is weakly organized, and is composed of a variety of structures, with distinct architectures and dynamics, which are nucleated and elongated by different factors, and bind to specific sets of actin-binding proteins that modulate their dynamics. Understanding how actin is distributed between these structures is critical to understand the biology and mechanics of the actin cytoskeleton and therefore its role in processes ranging from morphogenesis and cell division to endocytosis and polarization. In the proposed work, taking advantage of single-molecule techniques I developed during my previous post-doc, I will use the early C. elegans embryo as a model system to tease out the general rules and the critical regulatory elements that control actin homeostasis. I will follow three major directions: (1) analysis of actin dynamics in a steady-state system, the 1-cell stage embryo during maintenance phase, (2) analysis of the mechanisms underlying actin homeostasis in face of changes in the concentration landscape and biochemical properties of the players and (3) analysis of the modulation of actin dynamics and homeostasis during early embryonic development and between different cell types. I propose here a research program to expand our knowledge of how the structure of the actin meshwork is regulated and controls morphogenesis in embryonic cells, and in particular the mechanical properties of the cell. Importantly this program explores how modulations of the concentrations of actin-interacting proteins impinge on the dynamics of the actin cytoskeleton as a whole, and should improve our understanding of the general mechanisms that underlie the regulation of the actin cytoskeleton machinery, and how deregulations may be responsible for the onset of specific behaviors of the actin cytoskeleton that may eventually result in the development of cancer-like cellular behaviors.

Keywords: cell biology,actin dynamics,homeostasis,cortex,single molecule imaging,simulation