Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Computational Investigations of Allostery between Proteins and Nucleic Acids in CRISPR-Cas9 (Allosteric-CRISPR)

Framework: Horizon 2020 — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Starting grants
Action type: ERC actions
Panel: Physical and analytical chemical sciences
Panel description: Analytical chemistry, chemical theory, physical chemistry/chemical physics
Countries involved: Germany
Call: ERC STARTING GRANTS (ERC-2020-STG, 2020)
Start date: 2022-08-01
End date: 2027-07-31
Duration (months): 60.0
Total project cost (EUR): 1399632.0
EU funding (EUR): 1399632.0
Participants: 1
Project status: CLOSED

Allostery is a fundamental property of proteins, which regulates biochemical information transfer between spatially distant sites. Many emerging allosteric targets are large protein/nucleic acid complexes responsible for genome editing and regulation, whose underlying signaling remains poorly understood. Here, we focus on CRISPR-Cas9, a large nucleoprotein complex widely employed as a genome editing tool with enormous promises for medicine and biotechnology. In this system, an intricate allosteric signaling is suggested to span the multi-domain Cas9 protein and its associated nucleic acids, controlling the system’s function and specificity. However, in spite of extensive experimental characterization, the molecular basis for this allosteric response are largely unknown, hampering also efficient engineering for improving its genome editing capability. Allosteric-CRISPR will investigate the allosteric regulation in CRISPR-Cas9 by introducing a novel synergistic approach. This will implement the combination of state-of-the-art theoretical methods, including enhanced and multiscale approaches based on classical and ab-initio methods, with network models derived from graph theory and novel centrality analyses that are emerging as powerful to investigate allostery. This will create an innovative protocol that will enable determining the allosteric network of communication over multiple timescales, as well as the relation between allostery and catalysis, which remains unaddressed through classical approaches. This novel way to describe allostery can impact future studies of large nucleoprotein complexes, including newly discovered CRISPR systems, which are governed by similar allosteric rules and hold tremendous potential for genome editing. Finally, by delivering fundamental knowledge on the basic mechanisms underlying genome editing, Allosteric-CRISPR will help the design of improved genome editing tools, impacting their application across the field of life sciences.

Keywords: allostery,crispr cas9,protein,nucleic acid interactions,molecular dynamics
Innovation services for enhancement innovation SMEs capacities in Madrid region (INNMADRIMASD 2)

Framework: Horizon 2020 — Industrial Leadership
Programme: Innovation in SMEs
Action type: Specific Grant agreement
Countries involved: Spain
Call: Enhancing the innovation management capacity of SMEs (H2020-Adhoc-2014-20, 2014)
Start date: 2015-01-01
End date: 2016-12-31
Duration (months): 24.0
Total project cost (EUR): 230950.0
EU funding (EUR): 230950.0
Participants: 8
Project status: CLOSED

“Innovation services for SMEs innovation capacities enhancement in Madrid Region- INNMADRIMASD 2” is a proposal addressing the topic ‘Enhancing SME innovation capacity by providing better innovation support “under Horizon 2020. INNMADRIMASD 2 will provide innovation services to SMEs in Madrid through the Enterprise Europe Network as a suitable structure to provide in depth support services to SMEs in the area of innovation management. Furthermore, beneficiaries of the SME Instrument under Horizon 2020 will be entitled to receive specific targeted support from the Enterprise Europe Network to support coaching assignments addressing specific innovation weaknesses for Madrid based SMEs.

Keywords: innovation services,sme instrument,coaching,innovation support,enterprise europe network
INDUSTRIALISED DURABLE BUILDING ENVELOPE RETROFITTING BY ALL-IN-ONE INTERCONNECTED TECHNOLOGY SOLUTIONS (INFINITE)

Framework: Horizon 2020 — Industrial Leadership
Programme: Nanotechnologies, Advanced Materials, Advanced Manufacturing and Processing, and Biotechnology
Theme: Technologies enabling energy-efficient systems and energy-efficient buildings with a low environmental impact
Action type: Innovation actions
Countries involved: Austria,Italy,Italy,Italy,Italy,Spain,Spain,Germany,Germany,Austria,Switzerland,Slovenia,Slovenia,France,France,France,France,France,France,France,Netherlands,Netherlands
Call: Industrialisation of building envelope kits for the renovation market (IA) (H2020-NMBP-ST-IND-2020-singlestage, 2020)
Start date: 2020-11-01
End date: 2025-04-30
Duration (months): 54.0
Total project cost (EUR): 10141034.03
EU funding (EUR): 7999286.89
Participants: 30
Project status: SIGNED

INFINITE aims at increasing the market penetration of industrialized all-in building envelope kits for the deep renovation, as competitive, reliable, stakeholders-accepted and life-cycle-based sustainable approach contributing to decarbonization of the EU building stock. INFINITE will work to narrow the gap towards the full adoption of an industrialized renovation approach developing a set of multi-user and multi-disciplinary tools, and all-in-one industrialised envelope technologies, based on robust business, meeting the demand side requirements, by keeping the focus on final users and all the stakeholders in the value-chain. The INFINITE development drivers are: (i) cost and timing reduction, (ii) Life Cycle perspective, (iii) design for assembly and disassembly, considering end-of-life residual value and the Construction-Demolition-Waste topics, (iv) low carbon impact materials adoption, (v) stakeholders-centered approach to assure concept acceptance. Moreover, INFINITE will setup an open structured knowledge hub and an organized network of entities as hard-facts based dissemination, for the demonstration of the coupling of digital and industrialised approach. Thanks to INFINITE, EU construction sector can take the chance to move forward towards a long-lasting decarbonisation of the building stock, exploiting the advantages of the “Renovation4.0” (industrialisation + digitalisation) as new generation of industrialised renovation process. The underpinned ground-breaking technologies, tools and business models, will impact the whole value-chain, being able to trigger the deep renovation market activation. Demo-cases can validate the mentioned Renovation4.0 approach, while the consortium already well-established commercial channels in EU that will facilitate an extensive replication potential and effective results exploitation.

Keywords: coupled digital industrialised deep renovation,life cycle perspective,stakeholders centered approach,whole value chain optimisation
New technology and strategy for a large and sustainable deployment of second generation biofuel in rural areas (BABET-REAL5)

Framework: Horizon 2020 — Societal Challenges
Programme: Secure, clean and efficient energy
Theme: Alternative fuels and mobile energy sources
Action type: Research and Innovation actions
Countries involved: Spain,Argentina,Uruguay,Germany,France,France,France,France,France,France,Denmark,Mexico,Portugal
Call: Developing next generation technologies for biofuels and sustainable alternative fuels (H2020-LCE-2015-1-two-stage, 2015)
Start date: 2016-02-01
End date: 2020-01-31
Duration (months): 48.0
Total project cost (EUR): 5995198.66
EU funding (EUR): 5573643.51
Participants: 19
Project status: CLOSED

The business model currently under development for second generation ethanol is a replication of the model used for first generation which is plants with massive annual production capacities. Such high production rates require high capital investment and huge amounts of biomasses (250-350,000 tons per year) concentrated in small radius catchment areas to afford transportation costs (50 km). Under such conditions, opportunities for installing plants in most rural areas in Europe and worldwide are scarce. The objective of the project is to develop an alternative solution for the production of 2G ethanol, competitive at smaller industrial scale and therefore applicable to a large amount of countries, rural areas and feedstocks. The target is to reach technical, environmental and economical viabilities in production units processing at least 30,000 tons equivalent dry biomass per year. This approach will definitely enlarge the scope of biomass feedstocks exploitable for the production of biofuel and create better conditions for the deployment of production sites, to the benefit of rural areas in Europe and worldwide. The main concept underpinning the project relies on a new biomass conversion process able to run all the steps from the pretreatment of the raw material to the enzymatic pre-hydrolysis in one-stage-reactor under mild operating conditions. This new process recently developed to TRL 4, offers the most integrated and compact solution for the conversion of lignocellulosic biomass for the production of ethanol developed so far, and it will lead to reduced capital and operation expenditures. The new process will be developed to TRL 5 in the project with the goal of achieving satisfactory technical, environmental and economical performances in relevant operation environment. The project will investigate and select business cases for installations of demonstration/first-of-a-kind small-scale industrial plants in different European and Latino American countries.

Keywords: second generation ethanol,biogas,lignocellulosic biomass feedstock,biomass pretreatment,enzymatic hydrolysis,c5 c6 cofermentation,life cycle assessment
INNOVOUCHER: IMPLEMENTING A EUROPEAN LABEL FOR INNOVATION VOUCHERS (INNOVOUCHER)

Framework: Horizon 2020 — Industrial Leadership
Programme: Innovation in SMEs
Action type: Coordination and support actions
Countries involved: Italy,Spain,Belgium,Poland,Romania
Call: European label for innovation voucher programmes (H2020-INNOSUP-2014-6, 2014)
Start date: 2015-05-01
End date: 2019-04-30
Duration (months): 48.0
Total project cost (EUR): 1513343.75
EU funding (EUR): 1513343.75
Participants: 5
Project status: CLOSED

The main objective of INNOVOUCHER project is to develop a European Label for Innovation Voucher programmes, by defining a new model of innovation voucher programme supporting the transnational exchange of innovation services and the procedures of the award of the European Label to those programmes complying with the defined model. The project will foster the transnational collaboration based in innovation vouchers by increasing the range of services offered in the vouchers scheme to include innovation transnational services and the definition of a procedure of automatic recognition of foreign providers, thus increasing the access of SMEs to knowledge and technology from all across Europe. The project will define all the aspects of the model (vision, services catalogue, service providers, communication plan, voucher mechanism and the elements for the efficient management of the model), their minimum requirements to enable the European Label award and the European Label system, based on the experience of the consortium partners on the innovation vouchers management. The new model will be tested in real environment in a pilot project by the launch of an innovation voucher’s call in five regions to support only transnational exchange of innovation services. Lessons learnt during the pilot project, jointly with the feedback from other regions from outside of the project will contribute to improve the defined model. The outcome of the project will consist on a guideline of recommendations on the European Label implementation that will provide the European Commission the tools, procedures and model of entity to manage a future European Label system. The guideline will be delivered in two phases: a first draft after the first year of the project and a reviewed version after the completion of the pilot project, at the third year. This guideline pretends to contribute the future policies to be developed by the European Commission on innovation support field.

Keywords: transnational collaboration
Towards new frontiers for distributed environmental monitoring based on an ecosystem of plant seed-like soft robots (I-Seed)

Framework: Horizon 2020 — Excellent Science
Programme: Future and Emerging Technologies (FET)
Theme: EIC Pathfinder
Destination: Emerging themes and communities
Action type: Research and Innovation actions
Countries involved: Italy,Germany,Italy,Italy,Cyprus,Netherlands
Call: Environmental Intelligence (H2020-FETPROACT-2020-2, 2020)
Start date: 2021-01-01
End date: 2024-12-31
Duration (months): 48.0
Total project cost (EUR): 3997521.25
EU funding (EUR): 3997521.25
Participants: 6
Project status: SIGNED

I-Seed aims at developing a new generation of self-deployable and biodegradable soft miniaturized robots, inspired by the morphology and dispersion abilities of plant seeds, to perform a low-cost, environmentally responsible, in-situ detection of key environmental parameters in air and topsoil. Seeds dispersal strategies are driven by the spontaneous, often reversible, anisotropic deformation of their tissues upon swelling or drying of cell walls, and do not require any control or internal energy supply. Following a bioinspired design, these skills will be implemented into seed-like robots by using multi-functional biodegradable materials and natural morphological features, which endow them with the ability to fly and disperse via natural vector-mediated diffusion in air, or to respond to humidity variations to move on terrain surface and self-penetrate in topsoil. Sensing will be based on diffusion, absorption, and structural changes of parts of the robots made from sensor materials with a chemical transduction mechanism and fluorescence-based optical readout. Recordings of temperature, humidity, CO2 and mercury (Hg2+) will be executed at air-soil interface. Deployment of the I-Seed robots and reading of the acquired measurements will be performed using drones. Seed-drone communication will rely on optical signaling and fluorescence based on Light Detection and Ranging technology (fLiDAR), and using photonic "tags" to impart different type of sensors and robots a unique response. Algorithms will be developed such that drones can map the robots in air and on the ground. By merging bioinspired soft robotics, material science, nanocomposite technologies, and environmental science, I-Seed will address the specific challenge by building a radically new dynamic scenario for analyzing and monitoring air and topsoil environments and their interface, extending environmental sensor networks and filling existing gaps of data analysis systems.
SUPercontinuum broadband light sources covering UV to IR applications (SUPUVIR)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Innovative Training Networks (ITN)-European Training Networks
Action type: Marie Skłodowska-Curie actions
Panel: Information Science and Engineering
Countries involved: Austria,Australia,Germany,Austria,Switzerland,Finland,Finland,Poland,France,France,France,France,United States,Denmark,United Kingdom,Denmark,United Kingdom,Denmark
Call: Innovative Training Networks (H2020-MSCA-ITN-2016, 2016)
Start date: 2016-10-01
End date: 2020-09-30
Duration (months): 48.0
Total project cost (EUR): 4017699.36
EU funding (EUR): 4017699.36
Participants: 24
Project status: CLOSED

SUPercontinuum broadband light sources covering UV to IR applications (SUPUVIR) will combine the efforts of 6 academic and 4 non-academic beneficiaries to train 15 ESRs for the growing industry within SC broadband light sources, giving them extensive knowledge in silica and soft-glass chemistry, preform design and fibre drawing, linear and nonlinear fibre and waveguide characterization, nonlinear fibre optics, SC modelling, SC system design, patent protection, and in-depth knowledge of a broad range of the main applications of SC high-power broadband light sources. The strong blend of academic and non-academic sectors in the consortium will give the ESRs a unique chance to develop a wide set of technical and transferrable skills, thus preparing them for long-time employment in the sector (academic or industry). Scientifically, SUPUVIR aims at solving current challenges preventing SC light sources from taking over key market shares or from being used for cutting-edge research . Specifically the objectives are to reduce noise and increase pulse energy of SC modules, as well as investigate SC generation in emerging wavelength regimes (UV and mid-IR) including fabrication of novel fibres and waveguides, and finally using SC sources for applications as to gain valuable knowledge of application requirements. This research and development will give improved SC sources and SC spectra enabling new science and applications for optical imaging, spectroscopy, sensing and control, e.g. optical coherence tomography, IR multimodal spectroscopy, confocal and fluorescence microscopy, photoacoustic imaging and food quality control.

Keywords: supercontinuum generation modelling and experiments,fibre design and fabrication,fibre glass material chemistry and preform fabrication,imaging and spectroscopy with supercontinuum sources
Composition and Mechanism of the DNA-uptake Pilus of Vibrio cholerae (CMDNAUP)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Switzerland
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2015, 2015)
Start date: 2016-09-01
End date: 2018-08-31
Duration (months): 24.0
Total project cost (EUR): 175419.6
EU funding (EUR): 175419.6
Participants: 1
Project status: CLOSED

Horizontal gene transfer (HGT) allows rapid bacterial evolution including the spread of genes encoding antibiotic resistance and virulence factors. The Gram-negative bacterium Vibrio cholerae is an important human pathogen that causes the pandemic disease Cholera. In its natural aquatic environment growth on the chitinous exoskeletons of zooplankton initiates the development of ‘Natural Competence’, a widespread and key form of HGT that allows bacteria to take up free DNA from the environment. DNA uptake involves a sophisticated nanomachine known as a Type IV Pilus (TFP), which forms polymeric extensions from the cell surface, are ubiquitous throughout bacteria and play a wide range of other roles such as surface motility and attachment. Notably, this machinery is conserved in other naturally competent bacteria including in several important human pathogens, implying a common mode of action. Work on this machinery has mainly been done in Gram-positive models like Bacillus subtilis but had failed to visualise an uptake pilus. Recently, the Blokesch lab visualised a DNA uptake pilus extending from the outer membrane of V. cholerae and determined the minimal known components needed for its assembly. However, we still know almost nothing about how this machinery actually works to bring DNA into the cell. To answer this important question and elucidate the underlying molecular mechanisms we will follow two main objectives. 1. We will determine the composition of purified DNA-uptake pili and investigate the functions of the identified proteins. 2. We will combine innovative genetic and cell-biological approaches to investigate the mechanism of DNA uptake. The Blokesch lab at EPFL has pioneered genetic and cell biological methods for studying competence in V. cholerae and has state-of-the-art equipment and infrastructures that offer the prospective fellow the maximum chance of success and the best-possible training through the research.

Keywords: horizontal gene transfer,type iv pilus,vibrio cholerae,cellular microbiology
Restoration of motor dysfunction in vivo through nanomaterials based devices (NanoZfish)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: United Kingdom
Call: Individual Fellowships (H2020-MSCA-IF-2016, 2016)
Start date: 2017-06-01
End date: 2019-05-31
Duration (months): 24.0
Total project cost (EUR): 195454.8
EU funding (EUR): 195454.8
Participants: 1
Project status: CLOSED

Spinal cord injury (SCI) and amyotrophic lateral sclerosis (ALS) are nervous system maladies that impair the function of spinal central pattern generators for locomotion. These disorders carry a significant social and economic burden, owing to a lack of effective therapeutic treatments. In the field of nanotechnology, carbon nanotubes (CNT) have shown outstanding promise for the improvement of neuronal function in vitro and are thus ideal candidates for repairing or ameliorating spinal defects associated with these disorders. The ultimate goal of this project is to develop CNT as a tool for restoring motor dysfunctions associated with SCI and ALS. Specifically, I will use zebrafish larvae as an in vivo model for testing the therapeutic efficacy of this innovative class of implantable devices. I will implant CNT and graphene based devices in healthy, spinal cord lesioned and ALS zebrafish models and test their effects on spinal cord function with a range of powerful in vivo techniques. My project will address three specific aims. First, biocompatibility of implanted devices will be assessed using in vivo patch clamping of spinal motoneurons in healthy fish implanted with nanodevices. Second, I will implant CNT into fish that have been subjected to SCI. Nanomaterial-facilitated functional recovery will then be tested with in vivo patch clamping of spinal motor neurons, analysis of locomotor behaviour and confocal microscopy. Finally, I will use similar approaches to determine if implanted CNT can slow or reverse spinal cord defects in a SOD1 mutant zebrafish model of ALS. The project will advance our knowledge in an innovative field of bioscience research and help to inform the development of alternative approaches to the treatment of SCI and ALS. Embedded in an international environment, such as that of the University of Leicester, I will support my research activity through a greater level of independence, with the aim of publishing papers as senior author.

Keywords: spinal cord injury,amyotrophic lateral sclerosis,motor recovery,motoneuron,zebrafish,carbon nanotubes,nanomaterials,nanotechnology,in vivo patch clamping