Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
REVEALING MYOSIN'S POWER STROKE WITH HIGH-SPEED SCATTERING INTERFEROMETRY (RMPSHSSI)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-10-01
End date: 2015-09-30
Duration (months): 24.0
Total project cost (EUR): 221606.4
EU funding (EUR): 221606.4
Participants: 1
Project status: CLOSED

The fundamental goal of microscopic imaging is to visualize and identify small objects and observe their motion. Of the many available techniques, single particle tracking has been an immensely powerful tool in the life sciences for studying the motion of individual objects and thereby the function of a large variety biological processes. Despite considerable advances, following the motion of individual molecules on the relevant time and size scale has remained an insurmountable challenge. Here, we propose to construct a novel optical microscope based on interferometric scattering (iSCAT) detection that will bridge the considerable gap between the temporal and spatial accuracy provided by current single molecule methods. The primary objective of this work is to utilize the unique imaging properties of interferometric scattering detection to directly visualize and thereby understand the power stroke of Myosin V. Myosin V is a molecular motor, a cargo transporter that moves along actin filaments while hydrolyzing ATP. A wealth of knowledge concerning myosin’s function has been acquired over the past decades. Nevertheless, many of the fundamental questions concerning the mechanism of the power stroke for these motors remain, largely due to fundamental limitations associated with the employed techniques. In this respect, the application of high-speed iSCAT to study the motion of Myosin V will represent a significant step forward in our understanding of the functionality of this important motor. We aim that this work will establish iSCAT as a viable and powerful in vitro imaging platform with capabilities that are several orders of magnitude beyond what is currently possible with state of-the-art single particle tracking approaches. As a consequence, this proposal will include specifics with respect both the biological and the technical challenges involved with the proposed studies.

Keywords: single particle tracking,iscat,myosin v,molecular motors
Optimizing Selectivity in C-H Functionalization Through Computational Design (CHOPTOCOMP)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Incoming Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2012-IIF, 2012)
Start date: 2014-06-16
End date: 2016-06-15
Duration (months): 24.0
Total project cost (EUR): 231283.2
EU funding (EUR): 231283.2
Participants: 1
Project status: CLOSED

The activation of inert C-H bonds lies at the heart of organic chemistry. In particular C-H activation using transition metal catalysis has made a profound impact on complex molecule synthesis, but the area remains important for future discovery. At present the utility of synthetic methods based on C-H activation is hampered by the inherent difficulty of being able to selectively functionalize a single C-H bond in the presence of many others. Thus the ability to perform predictably site-selective C-H functionalizations on a given C-H bond in a complex substrate would be transformative for chemical synthesis. In this proposal we propose to perform computational studies on Pd-catalyzed C-H functionalization reactions, to uncover the inherent electronic bias of substrate structures on the site-selectivity. Calculations will be performed using density functional theory to characterize the mechanisms and catalytic cycle for Pd-catalyzed arylation of aromatic and heteroaromatic substrates. We will also develop quantitative models of reactivity and selectivity to deliver a greater understanding of the process, which will be used to generate predictions. The result will be a reliable predictive method with which to rationally design substrates and catalysts to deliver improved selectivities in C-H functionalizations.

Keywords: c-h functionalizations,computational study,c115 theoretical and computational chemistry,c112 catalysis
On the Correlation between Meta skills in Trainers and the Learning Increase in Participants of short-term Experiential Education Programmes (COMET)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-08-01
End date: 2015-10-30
Duration (months): 24.0
Total project cost (EUR): 231283.2
EU funding (EUR): 231283.2
Participants: 1
Project status: CLOSED

This IEF project will use a combined qualitative and quantitative approach to investigate the correlation between meta skills in Experiential and Outdoor Education (EE/OE) facilitators and the success of EE programmes (EEPs) as measured by participants and sponsors. The overall goal is to obtain more tangible information on which skills and personality attributes in educators most ideally support the development of key competencies in participants. The notion of transfer into school settings will receive particular attention in the study design. The project and IEF will be conducted in collaboration with Professor Peter Higgins and the Outdoor Education Department, University of Edinburgh, UK. The innovative feature of this project is that it is the first to investigate the impact of the factor 'human' on the learning outcome and it is expected to yield valuable insights into how to increase effectiveness and efficiency of EEPs. Also, in combination with the researcher's training, neurocognitive research methods will be implemented, which is an absolut novelty to EE/OE research and is expected to act as sign post for future investigations. EE is gaining notable weight not only in promoting social and personal competence in pupils, but also as assessment and training tool in HRM. The project aims to raise further awareness of the socio-economical value in the EE/OE approach to the commercial sector, which has a direct resale value to OEE providers as well as HRM groups in internationally acting companies and could also strengthen the European Community's competitiveness on the worldwide market. And finally, the IEF will be of great benefit to the researcher's medium and long-term career development by offering a set of unique and high quality training steps in scientific skills and general key competences that will aid her in attaining a leading research position, and thus contribute to the European Research Area by striving towards future quality research.

Keywords: s213 education,experiential education,meta skills,key competencies,facilitator,teaching approach,neurocognitive science,interdisciplinary field study
Functional analysis of SCOC and FEZ proteins in autophagy using mammalian cell models and zebrafish (SCOC AND FEZ)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-04-01
End date: 2015-03-31
Duration (months): 24.0
Total project cost (EUR): 221606.4
EU funding (EUR): 221606.4
Participants: 1
Project status: CLOSED

Autophagy is a conserved, highly regulated degradative pathway transferring cytoplasmic components in autophagosomes to lysosomes providing energy for cellular metabolism. In multicellular organisms autophagy is essential for tissue homeostasis and deficient autophagy is implicated in a broad range of diseases e.g. cancer and neurodegenerative disorders. To develop therapeutic strategies targeting autophagy a comprehensive understanding of the molecular protein machinery mediating and regulating autophagy is required. In a genome wide siRNA screen the host laboratory identified SCOC, a small Golgi protein, as a novel positive regulator of starvation-induced autophagosome formation. SCOC forms a complex with FEZ1, which also contains ULK1. FEZ1 negatively regulates autophagy and FEZ1 and the related FEZ2 associate with ULK1 and mTORC1 kinase complexes. The TOR signalling pathway is central in nutrient sensing. Starvation inactivates mTOR kinase leading to ULK kinase activation and autophagy induction. SCOC and FEZ also interact with the tumor suppressor UVRAG, a subunit of class III Beclin1-PI(3)K lipid kinase complexes essential for autophagy induction and progression. Autophagy is crucial for nervous system development and neuronal survival. Interestingly, SCOC and FEZ1 are highly expressed in neurons and required for axon elongation in C. elegans. I hypothesize that SCOC and FEZ are membrane-proximal scaffold and adapter proteins important in regulating and coordinating the three key autophagic signalling complexes, mTORC1, ULK1 and UVRAG-Beclin1-PI(3)K. I aim to understand the molecular details of this function and how they relate to vertebrate nervous system development and homeostasis. Our multidisciplinary approach (biochemistry, biophysics, cell biology, imaging and zebrafish techniques) characterizing SCOC and FEZ function may also impact development of new therapeutic strategies in targeting autophagy in disease.

Keywords: autophagy,scoc,fez1,fez2,ulk1,mtorc1,uvrag,beclin1,pi(3)k,zebrafish
Semantic integrAtion and reasoning Framework for pharmacovigilancE signals Research (SAFER)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-07-01
End date: 2015-06-30
Duration (months): 24.0
Total project cost (EUR): 269743.8
EU funding (EUR): 269743.8
Participants: 1
Project status: CLOSED

Adverse drug effects endanger patients’ safety and cause considerable extra healthcare costs. The procedures of drug surveillance and drug harm prevention are studied by pharmacovigilance. Several Information Technology (IT) methods are currently employed for conducting research as regards identification of pharmacovigilance signals. The current project (SAFER) will elaborate on exploiting and complementing evidence obtained from various types of existing pharmacovigilance signal sources through: (a) the semantic harmonization and mediation among the respective signal detection methods, and (b) the development of signal aggregation and reasoning mechanisms that will enable knowledge fusion of the obtained outcomes, introducing overall an integrated, semantic approach for obtaining and managing knowledge on pharmacovigilance signals. The proposed approach is particularly applicable, since signal generation is characterized by incomplete knowledge and uncertainty in the results obtained from all types of signal detection methods, thus, a synthesis of all possible signal sources is necessary. SAFER aims to properly detect true associations and minimize false positive findings, which are the major drawbacks of most signal detectors currently available, so as to reinforce the reliability and completeness of pharmacovigilance signals. Major expected results of SAFER constitute: (a) a generic knowledge model that will address the heterogeneity of the available signal detectors via their semantic harmonization, (b) an advanced decision support service for medication error prevention based on the introduced reasoning scheme, and (c) a semantically-enriched IT framework for integrated pharmacovigilance research. SAFER will be conducted in the INSERM - U872, Eq20 - group, a centre of excellence in the field of knowledge engineering and semantic technologies in e-health with major application domain pharmacovigilance.

Keywords: medical informatics,pharmacovigilance signal detectors,knowledge engineering,semantic integration,biomedical terminologies/ontologies,decision support systems
The Europeanisation of the Holocaust memory in Eastern Europe (EURHOLMEM)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Sweden
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-08-15
End date: 2014-08-14
Duration (months): 12.0
Total project cost (EUR): 137569.4
EU funding (EUR): 137569.4
Participants: 1
Project status: CLOSED

The Holocaust, i.e. the destruction of almost six millions of European Jews between 1933 and 1945, after having faded into oblivion in the late 1940s and 50s, has been a matter of developing national memories in Western Europe, Israel and the USA since the 1960s and a part of cosmopolitan and European memory since the 1990s. In Eastern Europe (EE), where the Holocaust had largely happened, its memory hardly developed until the end of the communist rule in 1989-91. The general objective of this project is to assess to what extent, how and why the cosmopolitan and (West) European memory of the Holocaust has contributed to the development of the Holocaust memory in EE since 1989. The research concerns primarily the former communist-ruled countries that joined the European Union in 2004 and 2004, with references to other post-communist countries in the east of the continent (except for Russia). The project aims (1) to assess how much national and cosmopolitan/[West] European Holocaust memory there is in the EE countries; (2) to analyse the Europeanisation of the Holocaust memories in the EE countries through European organisations: the Stockholm International Forum on the Holocaust, the Task Force for International Cooperation on Holocaust Education, Remembrance, and Research; the Council of Europe; the Organisation for Security and Cooperation in Europe; and, particularly, the European Union; and (3) to discuss the causes of slower development of the Holocaust memory in EE. The research involves: (1) the analysis of the Holocaust organisations, memorials, remembrance, education, and public awareness in EE; (2) the analysis of the participation of the EE countries in the Holocaust remembrance within selected European organisations, including the analysis of the Holocaust-related debates in and documents of the European Parliament; (3) the study of the politics of memory of selected EE countries in regard to the Holocaust, with reference to its history.

Keywords: europeanisation,memory,holocaust,eastern europe
Protein-RNA networks ALS (PRANA)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-08-01
End date: 2015-07-31
Duration (months): 24.0
Total project cost (EUR): 231283.2
EU funding (EUR): 231283.2
Participants: 1
Project status: CLOSED

Interactions between proteins and RNAs regulate processing, localization, decay and translational regulation of mRNAs. Recently, it was revealed that the number of putative RBPs in mammalian cells is about three times larger than appreciated. However, the exposed interaction network was inspected only in a single given state; furthermore the functional gene expression mechanism is yet unknown. In my proposed project, we aim to inspect the differences in global protein-occupancy on mRNAs and identify the critical remodeling in protein-RNA interactions that drives the subcellular localization and translation of mRNAs. Our goal will be to understand the misregulated RNA networks in Amyotrophic-lateral-sclerosis (ALS), a common neuromuscular disorder. We will study mouse and human embryonic stem cells and induced pluripotent stem cells of ALS patients, and brain tissue of mouse ALS models. Protein-RNA interactions will be studied by crosslinking the cells in different biological states, purifying RNPs from different cellular fractions high-throughput sequencing on iCLIP produced libraries, and mRNA translation will be studied with ribosome profiling. The combinatorial statistical inference and state-of-the-art computational approaches will be used to interpret the relevant dynamic RNA networks. This work will aim to reveal how normal and dysfunctional dynamic RNA regulation controls translation in motor neurons.
New Insights in Eating Disorders Paving the Way for Innovative Treatments (NIEDPWIT)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Duration (months): 24.0
Total project cost (EUR): 261384.6
EU funding (EUR): 261384.6
Participants: 1
Project status: CLOSED

My project aims at gathering new evidence to treat a major health concern: Eating disorders, including anorexia and bulimia nervosa and food bingeing leading to obesity. Following up on our parallel results showing an addictive component in these disorders and a dual role for serotonin (5-HT) in feeding, we postulate that the transition from the abusive consumption of food (food bingeing) towards excessive food deprivation (anorexia-like behavior), depends on the constitutive activity of the 5-HT-4 receptors (5-HTR4) and on changes from its inactive (R) towards its active state (R*) within the nucleus accumbens (NAc), a structure of the brain’s reward system. To demonstrate that the constitutive activity of 5-HTR4 in the NAc switches the direction of the addiction to food, I will modify the 5-HTR4 activity state using specific pharmacological treatments (from inverse agonist to overexpression) infused in the NAc of behaving brain-specific 5-HT deficient mice (Tph2-/-). Since my preliminary results show that Tph2-/- display feeding alterations (opposite in Tph2-/- and +/-), my first and second objectives will further characterize in detail their feeding pattern and its correlation with 5-HTR4 density in the NAc, body weight and fat storage. My third and fourth objectives test whether infusion of 5-HTR4 inverse agonist (decreased constitutive activity) and 5-HTR4 overexpression (increased constitutive activity) in the NAc induce respectively food bingeing and food refusal via the molecular cascade: cAMP-CART-FosB-ΔFosB. My experiments will show that controls of the “accumbal addiction pathway” by R and R* 5-HTR4 states are opposed and control the intake of food. Finally, I will realize a preclinical study and pave the way for the first clinical study using 5-HTR4 drugs to treat eating disorders. The realization of this innovative interdisciplinary project is a step further to cure eating disorders and will increase my professional maturity and European excellence.

Keywords: l512 psychiatric disorders,l508 behavioral neuroscience,l503 neurochemistry and neuropharmacology,anorexia / bulimia nervosa,binge-type eating,obesity,knockout mouse model,serotonin,5-htr4 receptor
Climate change effects on aquatic and terrestrial invertebrate assemblages in European rivers: the influence of duration of dry period in temporary rivers (CLITEMP)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Intra-European Fellowships
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2012-IEF, 2012)
Start date: 2013-05-03
End date: 2015-05-02
Duration (months): 24.0
Total project cost (EUR): 223778.4
EU funding (EUR): 223778.4
Participants: 1
Project status: CLOSED

Assessments of global patterns of climate change forecast a general increase of temperature and changes in precipitation. It seems that ﬂoods and droughts would become more severe and frequent across Europe, thus increasing the proportion of rivers with mediterranean characteristics in actually temperate areas. Therefore, climate change and increasing water demand will inevitably expand river drying in time and space. Hydrological effects include flow interruptions, loss of hydrological connectivity between river compartments and increased water residence times in extended sections of river networks. It has generated a renewed and urgent need to better understand the ecological structure and function of temporary rivers. It is recognised that the interruption of superficial flow plays an essential role in the macroinvertebrate aquatic communities, however few studies are focused in the influence of a gradient of flow intermittence on richness, density and traits of macroinvertebrate communities within temporary streams. Moreover, the importance of the dry river-beds by maintaing the diversity of aquatic and terrestrial assemblages, regulate the transfer and transformation of energy and materials, and define the resilience of the system, has been highlighted recently. The general objective of this research is to find out as the duration, extent and severity of the dry period will control both aquatic and terrestrial assemblages, to predict the effect of climate change in European rivers. The main specific objectives are: i) to analysis changes in community structure and composition, and biological traits of macroinvertebrate assemblages along a gradient of flow intermittence in temporary rivers, ii) to study the effects of different mesohabitats in macroinvertebrate communities, and iii) to investigate the effect of the expansion dry river bed period on terrestrial invertebrate assemblages and the throphic linkage between aquatic and terrestrial organisms.

Keywords: v201 climatology and climate change,european rivers,temporary rivers,aquatic invertebrates,terrestrial invertebrates,drought,flow intermittence,linkage aquatic-terrestrial phases