Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Cell cycle clock in nervous system and cancer (CYCLOCK)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-03-01
End date: 2015-02-28
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Cyclins play role in the cell division machinery and dictate the proceeding of cell cycle phases. D-type cyclins activity is required for progression through G1 phase, the critical step when cells steer the wheel to enter a new cycle of proliferation, to exit cell cycle and become quiescent or ultimately to differentiate. Generation of mice nullizygous for each of the three D-cyclin genes revealed their physiological impact as well as protective effect against cancer. Cyclin D1 is important during nervous system development, in particular for the retina and the cerebellum. However, cyclin D1 is also directly involved in the proliferative response of estrogen receptor alpha positive breast cancers and over-expressed in HER2 (ERBB2) dependent breast cancers. Recently, we reported a genome-wide function for cyclin D1 in transcriptional regulation, acting physiologically during retinal development. To affect protein production, it has been reported that transcriptional regulatory events need to be coupled with the nuclear export machinery, optimizing transfer of neo-synthesized RNAs to the cytoplasm. This suggests transcription and RNA export are spatially and temporally interconnected rather than stochastic separated phenomena. Hence, we intend to define how cyclin D1 activity governs interactions between the nuclear export and transcriptional machinery during nervous development in comparison to cancer situation. This research project relies on innovative genetics thanks to tandem tagged (Flag-HA) cyclin D1 mice model, together with modern proteomics and genomics approaches. We explore the transcriptional loop operating during cerebellum development in comparison to medulloblastoma genesis, and we extend our study to ERBB2 and ER-dependant breast cancer on the other hand. Demonstrating the mechanism of action between D-cyclins, nuclear pore complexes and transcription factors in this context, will highlight new oncogenic cell cycle components of theranostic potential.

Keywords: anticancer therapy,neurobiology,clinical analysis,genomics,proteomics,molecular genetics,molecular markers and recognition,biomedical engineering,animal biotechnology,animal model,theranostic,oncology
Role of the tubulin posttranslational modifications and microtubule severing protein, katanin in the cilia central pair assembly. (CP ASSEMBLY IN CILIA)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Poland
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-08-01
End date: 2015-07-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Cilia are microtubule (MT)-based external cell extensions that perform sensory and locomotory functions. Disruption of ciliary beating leads to human disorders such as infertility, airways diseases and hydrocephalus. The central pair of MTs (CP) apparatus is required for proper cilia motility yet the molecular mechanism that regulates its assembly is unknown. Loss of function mutations of MTs severing protein katanin or changes in the levels of MTs posttranslational modifications (PTMs) result in assembly of short immotile cilia that lack CP. Tubulin PTMs affect activity of katanin toward cytoplasmic MTs but the role of katanin and tubulin PTMs in ciliogenesis is unknown. Thus our main goal is to determine the role of katanin and tubulin PTMs and their reciprocal relationships during ciliogenesis, and their effect on the assembly and/or stability of CP MTs. We intend to identify katanin ciliary localization domain and ciliary interacting proteins. We will use a well established model to analyze motile cilia, a ciliate Tetrahymena thermophila. We will take advantage of high resolution microscopy for localization studies. We will perform mutagenesis analysis of katanin and b-tubulin in order to identified the key amino acid(s) involved in ciliary localization and interactions between these two proteins in cilia. To determine if defects in cilia caused by b-tubulin or katanin mutations can be rescued by parallel mutations in katanin and b-tubulin, respectively and restore disrupted protein interactions we will mutate katanin (or b-tubulin) and express under inducible promoter in wild-type and loss of function mutation background of partner protein. The outcome of the project will have likely medical implications. The project links different aspects of my postdoctoral research and will enhance the research potential of the Host Organization, provide foundation for competitive research program and will become a stepping stone into building an independent research group.

Keywords: health sciences,cilia,central pair,tubulin,microtubules,posttranslational modifications,polyglutamylation,polyglycylation,katanin,ciliopathies
Protein Aggregation - a quantitative assessment (PROARGUS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Romania
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-03-01
End date: 2015-02-28
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Proteins aggregates are supramolecular ensembles capable to elicit significant biological responses and alter molecular reactions and quantitative, high throughput monitoring of the aggregation process is needed in high impact fields such as pharmacy and medicine. Very recent results show the possibility of ultrasensitive and conformation specific detection of proteins using sensors with Molecular Imprinted Polymers (MIP’s). Formed in the presence of target analyte, these polymers retain the memory of analyte’s shape. We propose to take advantage of the heightened and specific recognition properties of MIP’s to build an integrated sensing platform for the simultaneous, specific detection of monomer, dimer and a higher protein aggregate. This device will circumvent the need for prior separation of protein species and will be employed to monitor the aggregation of two proteins, lyzosyme and calcitonin. The innovative platform we envisage consists in nanoarrays with layers of MIP’s specific for monomer, dimer and a higher aggregate, deposited on the tips of gold nanopillars. Detection is based on the principle of impedance restoration when the “holes” in the polymer layer are filled by target protein species. Very sensitive detection of lyzosyme and calcitonin is anticipated and feasability of MIP micro arrays for monitoring the aggregation process will be assessed through a forced protein degradation study and rigorous validation against a reference method. The project takes a multidisciplinary approach to shed light on the kinetics of orientation of protein species at MIP “holes” and investigate non-specific adsorption, with additional knowledge to be gained through Atomic Force Microscopy, Electrochemical Methods and Surface Plasmon Resonance studies using state-of-the-art equipment available at host institution. Many answers to questions regarding aggregates themselves, their differences in shape and properties are expected to be provided in this way

Keywords: electrochemistry,pharmaceutical chemistry,physical sciences,sensor,nanoarray,protein aggregation,molecular imprinted polymer
Application Information Services for Distributed Computing Environments (AIS-DC)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Croatia
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-06-01
End date: 2015-05-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Cloud computing is emerging as a major computational platform for business as well as scientific computing. Cloud computing is abstracting traditional reliance on locally available hardware and its configuration into the infrastructure-as-a-service (IaaS) and software-as-a-service (SaaS) paradigms. In order to realize widespread adoption of cloud computing, consistent (ideally, well-defined) Quality of Service (QoS) needs to be delivered to the end users. Mandated by the cloud providers’ revenue model, cloud customers have to deal with the issue of multitenancy: placing of multiple individual customers on the same compute node. This results in variable performance of jobs executed on the cloud and thus unsatisfactory QoS. There is a need to enable a user to simply and quickly capture, compare, and monitor performance of their jobs within and across cloud computing providers. Extend Application Information Services (AIS) framework to allow individual services to run completely on cloud computing resources. Project will make AIS fully deployable on existing cloud frameworks. Provide a ready-to-deploy AIS distribution. AIS can be integrated into an otherwise existing cloud deployment as a standalone set of services independent of any other machine image or application instance. Perform a runtime analysis of a range of bioinformatics applications from different application categories to validate functionality delivered by AIS. Provide a complete application runtime performance data collection and retrieval solution for cloud computing. The solution easy to deploy, configure, and use by tools and individual users. By allowing individual users to have and easily control their own instances of AIS, any issues regarding data security and sharing are reduced. A user can choose to store any data desired without needing to worry how others might utilize it within a Virtual Organization (VO).

Keywords: technological sciences-cloud computing,application information services,distributed environments,virtual organisations,technological sciences
Understanding the cytotoxicity of aberrantly folded proteins in neurodegeneration (FOLDTOX)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: European Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Switzerland
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-03-01
End date: 2014-02-28
Duration (months): 36.0
Total project cost (EUR): 45000.0
EU funding (EUR): 45000.0
Participants: 1
Project status: CLOSED

An advanced systems biology approach will be applied to understanding the molecular mechanisms of neurodegenerative cytotoxicity, triggered by aberrantly folded proteins. Three established cellular models of cytotoxicty of the misfolding-prone proteins synuclein, TPD-43 and the Abeta peptide will be analyzed. In a discovery-phase, advanced proteome and phosphoproteome-wide screens,coupled to biochemica ltools for monitoring cellular toxicity and protein aggregation, will highlight biological processes sequentially affected by expression and aggregation of the misfolded proteins. The analysis will contribute to identifying triggering events and consequent phenomena, cellular responses that are specific to each protein and those associated to generic stress responses. Quantitative phosphoproteomics measurements will unravel signaling states associated to the cytotoxic mechanisms. Transcriptomics analysis will highlight protein changes that are not anticipated by gene expression data and suggest post-transcriptional regulation, possibly including protein aggregation phenomena. Emerging hypotheses will be validated by targeted proteomics based on selected reaction monitoring and biochemical and genetic experiments on the disregulated pathways. A set of protein and phosphoprotein markers will be selected that characterize the onset of cytotoxicity at different biological modules and stages and an assay will be designed that measures them in a fast and reproducible manner. This fingerprinting will be used to screen a set of chemical and genetic modulators, to characterize their mechanisms and strength. Overall, such multi-level approach will provide a global picture of the mechanisms that accompany the onset of proteinopathies and lead to neurodegenerative cell death, provide insight on the mechanism of action of specific modulators, ultimately setting us towards identifying the most suitable therapeutic strategies.

Keywords: proteomics,systems biology,yeast,misfolding,aggregation,neurodegenerative diseases
Optogenetic investigation of GABAergic interneurons in the limbic system during reward and addiction (OPTO-REW)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Sweden
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-03-01
End date: 2015-02-28
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Many psychiatric disorders involve dysfunction of the limbic system that is manifested in a reduced ability to experience pleasure and learning and memory impairment. Dopamine is important in the limbic system, which includes structures such as the nucleus accumbens, ventral tegmental area and prefrontal cortex. Drugs such as opiates and cocaine, but also antidepressant agents, and deep brain stimulation can possibly recruit certain types of interneurons, whose function is to regulate the firing and integration of information in local circuits. Functional study of local inhibitory interneurons of two classes, parvalbumin and somatostatin interneurons, using optogenetics and mouse genetics are the main focus of this proposal. It is of great interest to better understand the function of distinct neuronal classes during reward-related behaviors, and to identify how dopamine neurtransmission in specific neuronal classes regulate behavior. I propose to apply optogenetics to study the functional impact of GABAergic interneurons during reward-related behaviors. Functional studies of the limbic system have previously been limited to pharmacological approaches or lesion studies that provide poor cell-type specificity and low temporal resolution. Expression and control of light-activated channels using genetic methods in mice allows us to dissect the function of each neuronal class during awake behavior with millisecond resolution. The proposed research can provide important information on the function of discrete neuronal classes and their specific receptors, thereby providing the foundation for rational design of novel therapeutics targeting the limbic system.

Keywords: health sciences,neurobiology,neurophysiology,limbic system,optogenetics,interneurons,gabaergic,striatum,dopamine
The role of glycosphingolipids in development (GSL IN DEVELOPMENT)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-03-01
End date: 2013-09-30
Duration (months): 30.0
Total project cost (EUR): 62500.0
EU funding (EUR): 62500.0
Participants: 1
Project status: CLOSED

Glycosphingolipids have been implicated in the development of various human pathologies, such as cancer, obesity, diabetes or Alzheimer diseases. Their wide implication in cellular membrane architecture and cellular signaling network and metabolism has however made difficult the establishment of an integrated and accurate understanding of their role in vivo. The current proposal aims to understand such a role by taking advantage of a highly versatile system model, the Drosophila melanogaster. By combining sophisticated genetic and biochemical approaches with cutting edge biophysical strategies, such as FRET or FRAP technologies, this proposal intends to 1) determine how GSLs affect the dynamic organization of membranes at the nanoscale resolution in vivo, 2) study the GSL impact on cellular signaling and 3) polarity and finally 4) uncover some putative molecular regulators of GSL function in vivo. Preliminary work in Drosophila embryos allowed us to demonstrate that the absence of core GSLs in two related lethal mutants, egghead (egh) and brainiac (brn), leads to a surprisingly specific phenotype consisting in an increased number of proprioceptive organs. During the eclosion process, flies lacking core GSLs are unable to organize their movements and dies within the pupae case. Interestingly, Brn protein expression in brn flies using the UAS/GAL4 system in proprioception organs rescues their phenotype. We hence intend to take advantage of these rescue conditions to screen for molecular factors allowing to compensate the absence of core GSLs. Finally, the increased in proprioceptive organs being typically associated to an upregulation of the EGFR pathway, we aim to understand its genetic relationship of brn and egh mutations. This comprehensive characterization of the role of core GSLs in vivo will certainly constitute an important step in order to further evaluate the nature of their function in Drosophila models of human diseases in the near future.

Keywords: glycosphingolipids,rafts,drosophila,development,signaling network,egfr,genetic screen,fret,chordotonal,drug discovery,system biology
Painting the landscape of translational control of gene expression through meta-analysis of genome wide data sets (TRANSLATOMICS)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Sweden
Call:  (FP7-PEOPLE-2010-RG, 2010)
Start date: 2011-04-01
End date: 2015-03-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Translational control determines the rate at which mRNAs are synthesized into proteins; regulates biological processes ranging from apoptosis to memory; and shows deregulation in diseases such as fibrosis and cancer. In cancer, dysregulated translational control is associated with worse prognosis in most sold cancer. Despite this, integrated genome wide knowledge of translational control and its regulatory mechanisms is lacking. My new methods for data analysis and mechanistic discoveries allow, for the first time, efficient genome wide studies of translational control. By using all publicly available genome-wide data sets and these methods we will characterize the landscape of translational control by identifying genes and pathways that are regulated translationally; groups of genes that constitute functional translational modules and show co-regulation across many biological conditions; key genes that are highly connected (nodes) and under translational control; network structures that are regulated at the translational level; and mechanisms that mediate such regulation. This information will be used to compare many biological processes to generate an integrated perspective of translational control. Through functional experiments employing both gene specific and regulatory mechanism focused approaches we will link translational control, genes, mechanisms for regulation and biological processes to unravel a new level of understanding of translational control. Such knowledge will be applicable in future studies of how dysregulation of translational control contributes to human disease.

Keywords: anticancer therapy,functional biology,computational biology,cell biology
GPS-EGNOS based Precision Agriculture using unmanned aerial vehicles (FIELDCOPTER)

Framework: FP7 — Cooperation
Programme: Transport (including Aeronautics)
Destination: Support to the European global satellite navigation system (Galileo) and EGNOS
Action type: Collaborative project
Countries involved: Spain,Spain,Netherlands,Belgium,Belgium,Netherlands
Call:  (FP7-GALILEO-2011-GSA-1-b, 2011)
Start date: 2012-01-01
End date: 2014-03-31
Duration (months): 27.0
Total project cost (EUR): 967959.07
EU funding (EUR): 616979.0
Participants: 6
Project status: CLOSED

Unmanned Aerial Vehicles (UAVs) are an up-and-coming method in providing farmers with (near) real time sensing information for precision agriculture applications such as water stress monitoring, detection of nutrient deficiencies and crop diseases. FieldCopter provides state-of-the-art multi-spectral cameras on UAVs that deliver the right information in the right time on the right spot. The FieldCopter project develops a complete solution for UAV sensing. Existing components will be brought together and required high precision navigation of EGNOS will be added in order to create an autonomous flying camera that follows a predefined pattern. The consortium aims at a complete operational service and brings together the necessary competences assembled on the platform: the sensing aspects, the navigation expertise and the market knowledge. As the service is based on EGNOS, we can operate it anywhere in Europe. The partners combine market knowledge in the Mediterranean and higher latitudes, each with specific challenges. We will commercially exploit the results of this project in addition to the current business of the project partners. FieldCopter allows farmers to create economical and ecological benefits through optimal use of resources like water, nutrients (potassium, nitrogen) and crop protection agents. FieldCopter proves the potential of a European-wide precision navigation system and benefits from its operational continuity. EGNOS is a key enabler to develop this service.

Keywords: egnos uav uas precision agriculture fieldcopter fieldfact