Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Primary hypoxia signalling in plants (HYPOXSI)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: European Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Netherlands
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-06-01
End date: 2013-05-31
Duration (months): 36.0
Total project cost (EUR): 45000.0
EU funding (EUR): 45000.0
Participants: 1
Project status: CLOSED

The proposal presented here will allow the candidate to reintegrate, work on a cutting-edge scientific study and successfully develop his newly established independent research group. The project aims to investigate a key process in plant stress responses, namely how plants sense the oxygen availability and modify gene expression in response. Oxygen is a necessity for most forms of life. To be able to avoid the potentially life-threatening situation that arises upon reduced oxygen levels (hypoxia) most organisms have systems in place to measure intra-cellular oxygen availability. So far there is very limited knowledge on the primary hypoxia signalling pathway in plants. I will use state-of-the-art methods available for the model plant Arabidopsis thaliana to decipher this signalling pathway by identifying new components, determining the primary gene expression responses and generating a comprehensive network description. Together, the project targets an important issue in plant biology, is of excellent scientific and technical quality and will take place in a highly appropriate and stimulating environment. Thus, it will at the same time strengthen the researcher’s career perspectives and the host lab’s and Europe's leading position in plant research. Furthermore, the research project will generate fundamental scientific knowledge that in the longer term may contribute to the improvement of crop performance upon submergence and soil water logging and thus enhance food security.

Keywords: plant physiology,plant genetics,abiotic stress,signalling pathway
Differentiation dynamics in size-controlled embryoid bodies (DIFFEBIMG)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Israel
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-07-01
End date: 2014-06-30
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

In-vitro differentiation of embryonic stem cells into defined cell types is a field of immense importance for both regenerative medicine and for basic understanding of development. Promising clinical uses include generation of hepatocytes for treatment of liver failure and generation of cardiac progenitor cells or cardiomyocytes for treatment of acute coronary disease. Embryoid bodies (EB's), three dimensional aggregates of differentiating embryonic stem cells, have been the method of choice for in-vitro differentiation into several cell types, including motor neurons, hepatocytes and cardiomyocytes. I suggest a high-throughput live cell imaging approach for the study of EB differentiation dynamics. I will develop a microfluidic-based system for controlled EB formation, for generation and imaging of both uniform and variable size and shape EB’s. I will study correlates of specific cell fate differentiation and cell movement patterns by imaging large numbers of such systems, in conjunction with protein reporter and lineage tracing fluorescent constructs. I will focus on the conditions for appearance of cardiomyocytes: supporting cell types, spatial characteristics and signaling events. The project will enhance our basic understanding of cell movement and rules of differentiation during early development, and can lead to improved protocols of in-vitro differentiation.

Keywords: embryoid bodies,live cell imaging,stem cells,differentation
Role of Microtubule Polarity and Polarized Membrane Traffic in Directed Cell Migration (CELL POLARITY)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-05-01
End date: 2014-04-30
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

The ability of cells to polarize is crucial for development, wound healing, and neurotransmission. As many cellular polarity factors play central roles in disease (e.g. cancer, neurological dysfunction) understanding the molecular basis of cell polarity is of great importance to the biomedical sciences. One central aspect of cell polarity involves the regulation of the cytoskeleton and membrane-trafficking machinery, leading to the delivery of specific proteins and lipids to distinct cellular subdomains. This polarized membrane traffic seems important for cells that exhibit local cell growth, including migrating cells. Using advanced imaging approaches I showed that migrating cells preferentially deliver their secretory vesicles towards the leading edge (i.e. the front), and that this polarized delivery depends on intact microtubules (MTs). But how MTs and polarized membrane traffic contribute to cell migration remains unclear. Recent work on wound-edge migrating cells has identified factors that lead to distinct MT polarity phenotypes, i.e. MT stabilization and centrosome orientation, both of which could contribute to bias membrane traffic towards the front of the cell by either forming specialized vesicular tracks or by positioning secretory organelles in front of the nucleus. I will use interdisciplinary cell biological and state-of-the-art imaging and screening approaches to 1) investigate the mechanism of how MT polarity and polarized membrane traffic contribute to directed migration using known factors, 2) identify membrane trafficking factors that play a role in directed migration using automated image-based screening and 3) investigate the role of common traffic/migration factors in polarized membrane traffic and MT polarity. Further, I will implement ‘super-resolution’ microscopy to image the nanoscale localization of polarity factors in greater detail. These studies are aimed toward a more comprehensive understanding of cell polarity.

Keywords: health sciences (cell polarity,microtubules,migrations) technological sciences (microscopy,super-resolution,high-content imaging)
Development of a strategy to treat limb-girdle muscular dystrophy (LGMD2A) using combined cell and gene therapy strategies (LGMD2A)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Spain
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-07-01
End date: 2014-11-29
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 2
Project status: CLOSED

In Duchenne muscular dystrophy, several mutations can be corrected by the exon-skipping technique on cells. In limb-girdle muscular dystrophy type 2A (LGMD2A), however, all the mutations described to date do not seem to be good candidates for the RNA correction technology. The recently identified c.1782+1072 G&gt;C mutation in LGMD2A patients could be potentially repaired at RNA level. The principal aim of this project is to mask the effect of this mutation in stem cells from patients that can be derived to muscle and to test its therapeutic potential in a mouse model. This is the first step to be considered before planning a clinical trial in patients with this mutation. Our long term goal is to generate new advanced therapy protocols (gene and cell therapies) against LGMD2A, a muscular dystrophy that is specially prevailing in the population of the Basque region. To this end, we will pursue the following aims: 1. To develop a therapeutic approach for LGMD2A patients who carry c.1782+1072G&gt;C mutation. 2. Isolation, optimization of the in vitro culture and genetic correction of CD133+ cells from patients with this mutation. 3. Analysis of the efficacy of this combined therapeutic approach in murine models in vivo. In order to accomplish aims 1 and 2, we will develop a descriptive study with fibroblasts (from skin) and CD133+ cells (from blood and muscle) from donors, in which we will determine the efficiency, yield and myogenic potential for subsequent testing in mice. To accomplish aim 3 we will perform a double blind experimental approach in mice (wild type versus C3KO/scid and mdx/scid mice), using CD133+ cells with the most myogenic potential (from aim 2) as an in vivo therapy to determine the correction effect in the human calpain 3. This work would contribute to the treatment of LGMD2A patients who carry c.1782+1072G&gt;C mutation in calpain 3 and it could also be helpful for the treatment of other patients with similar type of mutations.

Keywords: gene therapy,degenerative diseases,cell biology,muscular dystrophy
ROLE OF PFKFB3 IN THE REGULATION OF THE CELL CYCLE AND TUMORIGENESIS (FP2ONCO)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Türkiye
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-06-01
End date: 2014-05-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Cancer is one of the leading causes of death in Europe and one of the highlighted issues in the 7th Framework Programme. Understanding the molecular mechanisms of cancer is expected to contribute to the development of better therapeutic approaches. In this project, the applicant proposes to examine the role of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) and its product, fructose-2,6-bisphosphate (F2,6BP), in the regulation of the cell cycle and tumorigenesis. Increased glycolysis is a key metabolic phenotype of cancer. Oncogenic stimuli increase F2,6BP, an activator of the first irreversible step of glycolysis. Previous studies have suggested PFKFB3 to be the dominant source of F2,6BP and the culprit behind the high glycolytic activity in tumors. The applicant has recently demonstrated that PFKFB3 localizes to the nucleus, where PFKFB3-mediated synthesis of F2,6BP stimulates cells to proliferate without a significant effect on glycolysis. He then discovered that F2,6BP in the nucleus activates cyclin-dependent kinases, decreases the expression of the cell cycle suppressor p27, and increases the expression of cyclin D3. These and other previous results obtained by the applicant suggest that tumor cells may need PFKFB3 and F2,6BP in the nucleus in order to couple glucose uptake with cell division activity in the nucleus. The objectives of the project are as follows: 1-To ascertain the role of PFKFB3 in the regulation of the cell cycle and cell cycle proteins; 2-To determine if there is a genetic/functional interaction between PFKFB3 and cyclin D3, and 3-To determine the in vivo interaction of PFKFB3 and p27, and whether PFKFB3 is required for tumor susceptibility induced by p27 deficiency. This project is expected to help the applicant continue his career in his host institution where he can apply the state-of-art techniques he has learned in the USA to contribute to the European Research Area in the field of cancer research.

Keywords: anticancer therapy,pfkfb3,6-phosphofructo-2-kinase,p27,glycolysis,cell cycle
Behavioural syndromes, division of labour and communication of identity in complex insect societies (IDENTITY CODE)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: European Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: France
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-06-01
End date: 2013-05-31
Duration (months): 36.0
Total project cost (EUR): 45000.0
EU funding (EUR): 45000.0
Participants: 1
Project status: CLOSED

Animals, just like humans, differ consistently in behavioural traits such as aggressiveness, exploration, reactivity. The study of behavioural syndromes (animal personalities), defined as sets of correlated behaviours across situations, helps in explaining the evolutionary significance of individual variation. However, most studies have focussed on few model organisms with no, or relatively little, social structure. Social insects represent one of the pinnacles of social evolution. Their well-organized societies are based on an often extensive division of labour, which includes reproduction and is frequently accompanied by morphological differentiation. The extent to which this labour partitioning can be implemented depends on the efficiency of communication strategies: in particular, the communication of identity. In social insects, communication of identity involves multiple levels of recognition: inter-individual, within-colony and between-colony level. How effectively individual or group identity can be detected may depend on the behavioural type of the receiver, but this has never been explored in social insects. This project builds on the synergy between the expertise of the applicant and the host to investigate the evolutionary significance and the ontogeny of individual variation in stimulus response allowing recognition at different levels by using different species of ants, which are among the most advanced and ecologically successful animal societies. The project combines behavioural observations, chemical analysis, electrophysiology, quantitative genetics, neurobiology and cognitive studies to achieve an interdisciplinary understanding of the forces that have shaped individual behavioural variation and collective division of labour in ants. The project’s implementation will be facilitated by the applicant’s network of high-profile collaborators and will thus have a significant impact (co-operation, professional integration) and transfer of knowledge.

Keywords: animal ethology,behavioural biology,evolutionary biology,social evolution,animal personalities,entomology,social insects
ENHANCED MRI PHYSICS SIMULATOR (MRISIMUL)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Greece
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-04-01
End date: 2014-03-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 2
Project status: CLOSED

We propose the design and development of an enhanced comprehensive computer simulation platform of MRI physics, which will integrate realistic aspects of the MRI experiment (such as motion and strain) from signal generation up to and including image reconstruction for the explicit purposes of 1) better understanding the mechanisms involved in artifact and contrast generation in cardiovascular MRI and 2) effectively teaching MRI physics to non-technical personnel. This comprehensive computer simulation of MRI physics and image reconstruction will allow us to better understand realistic artifact generating mechanisms in cardiovascular MRI and will help in defining exam protocols that are optimized for suppressing such artifacts without resorting to animal and/or human experimentation. This research will allow for detailed evaluation of cardiovascular pulse sequences and protocols such as Phase Contrast (PC) velocity mapping, myocardial tagging and Displacement Encoding with Stimulated Echoes (DENSE). With the design and development of the proposed simulator platform, the parameter space of different protocols will be tested and evaluated in detail without the time constraint imposed by experimentation in an MRI scanner. The simulations will be designed and developed with a variant of a four-vector formalism. A deforming model of the heart will be implemented as part of the simulator to allow visualization and analysis of imaging techniques that map myocardial motion and strain. Last, the simulation will allow for noise injection, respiratory motion modeling and multi-coil signal acquisition. We will apply this tool to allow researchers, technologists, physicians and students to visualize the effects of motion and/or strain, on tissue contrast, signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) in models of acute myocardial infarction. This will allow for further research in optimizing MRI protocols and also for training of non-technical personnel.

Keywords: biomedical engineering,magnetic resonance imaging,mri,cardiovascular mri,cmr,simulation
Resummation of Higher Orders in QCD Perturbation Theory (RESUQCD)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-06-01
End date: 2014-05-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

Collisions of highly energetic hadrons play a crucial role throughout much of nuclear and particle physics. They offer insights into the inner structure of nucleons and nuclei, allow studies and tests of the strong interactions and their fundamental theory, Quantum Chromo Dynamics (QCD), and also serve as invaluable tools in the search for physics beyond our &quot;Standard Model&quot; of the forces in nature. We propose to perform theoretical and phenomenological studies of hard hadronic collisions. Specifically, we plan to investigate the resummation of large logarithmic perturbative corrections to hard-scattering cross sections in QCD. Our studies would have wide-ranging applications for comparison to data from many present and future experiments in high-energy nuclear and particle physics, ranging from fixed-target experiments to the highest-energy colliders. We plan to improve previous resummation formalisms, develop new techniques and applications, and overall improve the theoretical description of the strong interactions at high-energy.

Keywords: nuclear and particle physics,strong interactions,qcd,perturbative calculations and resummation,parton distribution functions,spin structure of the nucleon
Role of the Cullin7 E3 ubiquitin ligase in insulin signaling and diabetes. (CUL7 AND DIABETES)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Germany
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2011-01-01
End date: 2014-12-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

This grant application is formulated to explore the biological role of the Cullin7 (CUL7) E3 ubiquitin ligase in insulin signaling and its implication for diabetes. My previous work led to the identification of insulin receptor substrate 1 (IRS-1) as the proteolytic target of the CUL7 E3 ligase and uncovered CUL7 as a novel regulator of insulin signaling. Based on these observations, I will employ experimental strategies as outlined in this proposal to delineate CUL7`s role for the cellular insulin response and its contribution to insulin resistance and type 2 diabetes.

Keywords: health sciences,diabetology,applied biology,biological sciences
Identification and screen of RNA Editing in the Human Genome (GENEDISCREEN)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: International Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: Israel
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2011-01-01
End date: 2014-12-31
Duration (months): 48.0
Total project cost (EUR): 100000.0
EU funding (EUR): 100000.0
Participants: 1
Project status: CLOSED

With the exception of infrequent random mutations (and immune cell receptors), it is widely believed that the genomic content should be fixed in an organism throughout its body and its lifespan. It is therefore clear why the discovery of proteins that can edit the RNA have generated much interest. However, only very few targets have been discover so far. I have recently made important progress in this area by developing novel methods to model and detect RNA editing in mammals. Using a combination of computational approaches and next generation sequencing based technology, I am able, for the first time, to perform large-scale comprehensive screen and sketch the “editosome” fingerprints. As an independent investigator, I will use the padlock probes technology followed by next generation sequencing to associate different diseases with altered editing levels and investigate the impact of RNA editing on genome evolution and development. To achieve these aims, my lab will use a combination of molecular biology and computational approaches. . It is my expectation that these studies will provide fundamental new insights into the mechanism of RNA editing and their role in human biology, within a timeframe of several years.

Keywords: genomics,genetic,rna editing,dna editing,next generation sequencing
The Renaissance Project: Towards a strategic orientation and associated toolkit to support global UCD that is appropriate and accountable to local culture (RENAISSANCE)

Framework: FP7 — People
Programme: Marie-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: European Re-integration Grants
Action type: Support for training and career development of researchers (Marie Curie)
Countries involved: United Kingdom
Call:  (FP7-PEOPLE-2009-RG, 2009)
Start date: 2010-06-01
End date: 2013-05-31
Duration (months): 36.0
Total project cost (EUR): 45000.0
EU funding (EUR): 45000.0
Participants: 1
Project status: CLOSED

Companies strive to develop products en masse for a global market. The challenge for companies is to maintain product quality, consistency and profitability, whilst ensuring that products meet and respect local and cultural norms. When technology design contravenes expectation, a product will fail, which can be disastrous not only for a company’s profits but dangerous in safety critical situations, and a potential source of structural instability in complexly interconnected sensitive industries such as finance. The Renaissance Project Project will establish the first steps towards a strategic orientation and associated toolkit to support global UCD that is appropriate and accountable to local culture. There are three main research objectives: i) to capture information related to knowledge management strategies for UCD; ii) to establish a toolkit to support appropriate design in disparate cultures; iii) to disseminate this knowledge within industry and academia. This research directly builds on the researcher’s prior Marie Curie ToK research, during which a design framework and evaluation tool was developed at Philips Research in Eindhoven to support socially intelligent design of products. By exploring new and pertinent research findings, capitalizing on existing international industry and academic partners (whilst simultaneously seeking to extend this network of interest), and addressing social need to ensure design fits culture, The Renaissance Project research findings promise to lead to new understanding and support for technology design and innovation.

Keywords: systems analysis and models development,social sciences,intelligent interfaces,media systems,interface technology,technological sciences,knowledge technology