Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Development of novel integrated sequencing methods to explore translation and its regulatory mechanisms in single cells (scTranslatomics)

Framework: Horizon Europe — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Advanced grants
Action type: ERC actions
Panel: Integrative B iology : from Genes and Genomes to S ystems
Panel description: For all organis ms: Genetics, epigenetics, genomics  and other ‘omics studies , bioinformatics, systems biology , genetic diseases , gene editing, innovative methods and modelling,  ‘omics for personalised  medicine
Countries involved: Netherlands
Call: ERC ADVANCED GRANTS (ERC-2021-ADG, 2021)
Start date: 2023-01-01
End date: 2027-12-31
Duration (months): 60.0
Total project cost (EUR): 2500000.0
EU funding (EUR): 2500000.0
Participants: 1
Project status: SIGNED

In recent years novel single-cell sequencing methods have allowed an in-depth analysis of the diversity of cell types and states in a wide range of organisms. Due to the continuous optimization of experimental and computational methods by many research groups, it is now possible to sequence the transcriptomes of thousands to millions of individual cells. Albeit an exciting development, transcription only covers the first step in the central dogma. The second step, the process of translation, is currently much harder to explore in single cells. Building upon existing ribosome profiling protocols, our laboratory recently majorly increased the sensitivity of these assays allowing ribosome profiling in single cells. However, currently no methods exist to determine the translation efficiencies in single cells and to correlate translation efficiencies to tRNA levels and their modifications, RNA bound proteins, and m6A methylation of mRNA, all major regulatory mechanisms of translation. The overarching goal of this proposal is to develop several novel multi-omics approaches to quantify translation in single cells by integrating information on ribosome position along the transcript, tRNA expression levels, tRNA modifications, RNA-bound proteins, and the m6A methylation status of the transcript. These technologies will open novel avenues to start exploring translation and its regulatory mechanisms with single-cell resolution. Whereas major discoveries have been made during the last decade by exploring the genome, the epigenome, and the transcriptome using single-cell sequencing approaches, translation, the major second step in the central dogma, is still very much unexplored at the single-cell level. I hope that the tools, presented in this proposal will provide the community with methods to explore, and ultimately understand, how translation contributes to the astonishing heterogeneity among single cells.

Keywords: single cell sequencing,single cell multiomics,single cell ribosomal profiling,scribo seq,single cell translatomics
Revealing the Landscape of Synaptic Diversity by Cell type- and Synapse-specific Proteomics and Transcriptomics (DiverseSynapse)

Framework: Horizon Europe — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Advanced grants
Action type: ERC actions
Panel: Neuroscience and Disorders of the Nervous System
Panel description: Nervous system development, homeostasis and ageing , nervous system  function and dysfunction , systems neuroscience  and modelling , biological basis of cognitive processes and of behaviour, neurological and mental disorders – In humans and all other organisms
Countries involved: Germany
Call: ERC ADVANCED GRANTS (ERC-2021-ADG, 2021)
Start date: 2022-08-01
End date: 2027-07-31
Duration (months): 60.0
Total project cost (EUR): 2498575.0
EU funding (EUR): 2498575.0
Participants: 1
Project status: SIGNED

Synapses differ in their shape, size, and function and exhibit ongoing plasticity during the development and modification of neural circuits. The structural diversity of synapses is largely known whereas the molecular diversity of synapses is much less well understood. While most synaptic molecules have been identified by immunolabeling and bulk proteomic approaches, the complement of proteins present at individual synapse types, as well as their stoichiometric relationships with other molecules remains unknown. Indeed, our current classification mostly relies on neurotransmitter/receptor phenotypes, leading to broad descriptors like “excitatory” or “inhibitory” synapses. Because the function of the synapse, as well as its ability to change, is largely determined by the quality and quantity of molecules that inhabit it, it is essential to understand synaptic molecular diversity. The aims of this proposal are to determine the proteomes and transcriptomes of genetically-identifiable synaptic populations in different brain areas and to determine the molecular diversity in these same synapse populations using transcriptomic analysis of individual synapses. We will also assess how these synaptic proteomes, transcriptomes and the transcriptomic diversity respond to plasticity. To study synaptic proteomes and transcriptomes we will use Fluorescence-Activated Synaptosome Sorting to purify different synaptic populations (excitatory, inhibitory, dopaminergic) from different brain areas. We will use the state-of-the-art methods optimized for quantitative transcriptomic and proteomic profiling of very small amounts of sorted synapses and develop a method, SynDrops, for the transcriptomic analysis of individual synapses. We will examine how synaptic proteomes and transcriptomes change during plasticity. These studies will reveal the landscape of synaptic diversity within synapse types and across brain areas and allow the field to probe “diseased” synapses in the future.

Keywords: synapse,proteomic,transcriptomic,local protein synthesis,mrna trafficking,mrna localisation
Assessing Feedback Responses of soil Erosion through the lens of variable Sediment connectivity during Extreme EveNts in semi-arid catchments. (AsFoRESEEN)

Framework: Horizon Europe — Excellent Science
Programme: Marie Skłodowska-Curie Actions (MSCA)
Theme: Marie Skłodowska-Curie
Destination: Postdoctoral Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Environment and Geosciences
Countries involved: Belgium,Australia
Call: MSCA Postdoctoral Fellowships 2022 (HORIZON-MSCA-2022-PF-01, 2022)
Start date: 2024-01-01
End date: 2027-03-31
Duration (months): 39.0
Total project cost (EUR): 288737.28
EU funding (EUR): 288737.28
Participants: 2
Project status: SIGNED

Soil resources in semi-arid regions are rapidly degrading, posing an imminent threat to food, water and livelihood security. Caveats in our understanding of geomorphological responses to extreme events are a major hindrance for attributing soil erosion and sediment flux dynamics to environmental drivers. Using the Burdekin and Makuyuni catchments as natural laboratories for semi-arid regions, the AsFoRESEEN project will assess feedback dynamics in soil erosion through the lens of variable sediment connectivity to test the hypothesis that extreme events can trigger regime shifts towards highly connected ephemeral gully networks. The proposed knowledge transfer strategies will bring the researcher’s scientific and analytical skills to the international standard, underpinning his ambition to combine academic and consultancy work within a leading European research institution. The researcher's unique skillset will be applied to develop novel approaches and integrate them with established techniques in an open-access diagnostic toolkit to support targeted soil- and water management interventions. Temporal dynamics in fine sediment and Phosphorous transport will be quantified using high-frequency sensors and sediment dating techniques. We will be the first to evaluate the use of secondary weathered metal species as tracers, providing a new pathway for attributing the contribution of gully erosion in deeply weathered or alluvials soils. Stream monitoring and sediment source tracing outputs will be integrated in a dynamic sediment budget to elucidate non-linear geomorphological responses to extreme events and land use changes. As a source of innovation, we will couple a machine-learning gully quantification tool with a dynamic catchment model, wherein gullies are both a direct source of sediment and a driver of changing sediment connectivity. The hybrid model will be used to test the efficacy of gully remediation strategies under current and future climatic conditions.

Keywords: gully remediation fluvial geomorphology regime shifts water quality high frequency sensors sediment tracing machine learning hydrochemical catchment models great barrier reef lake manyara
Searching for Oil Spills on Sea Surfaces (SOSeas)

Framework: Horizon Europe — Excellent Science
Programme: Marie Skłodowska-Curie Actions (MSCA)
Theme: Marie Skłodowska-Curie
Destination: Postdoctoral Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Environment and Geosciences
Countries involved: Netherlands,France
Call: MSCA Postdoctoral Fellowships 2022 (HORIZON-MSCA-2022-PF-01, 2022)
Start date: 2023-09-01
End date: 2026-02-28
Duration (months): 30.0
Total project cost (EUR): 187624.32
EU funding (EUR): 187624.32
Participants: 2
Project status: SIGNED

Oil spills rapidly spread on sea surfaces covering wide areas, assuming different appearances and thicknesses. The faster the actions to detect, stop, and contain the released oil from spreading, the higher the Oil Spill Response (OSR) success rate. Since, clean-up effectiveness is higher over thicker layers of oil - referred to as actionable oil - detecting these regions is crucial to enhance oil recovery efficiency, thus minimizing environmental and socio-economic impacts. The objective of "Searching for Oil Spills on Sea Surfaces" (SOSeas) project is to develop an artificial intelligence-based system to extract relative oil thicknesses by using multifrequency and multiresolution Synthetic Aperture Radars (SAR). Aerial reconnaissance is currently the most common method to estimate the extent, thickness, and volume of oil spills. However, it is subjective, biased and imprecise, demanding well-trained experts to visually estimate the extent of an oil slick and distinguish different oil appearances. Conversely, SAR are key-operational sensors for oil pollution monitoring, offering a synoptic view over affected sites, acquiring images during day and night regardless of weather conditions. The use of SAR data to detect the location and extent of oil pollution, as well as to discriminate it from false alarms has been well-researched. However, oil slicks characterization is under-explored, but a promising, new, and highly innovative research area, owing to the increasing availability of free SAR data, the development of powerful learning algorithms combined with high-performance computing advances. An automatic system well-trained to recognize patterns related to qualitative thickness ranging will indicate the actionable oil regions. These outputs can offer a less subjective and more precise oil pollution assessment than that of visual reconnaissance, improving situational awareness in time to guide trustworthy decision-making during clean-up operations.

Keywords: remote sensing synthetic aperture radar machine learning ocean observation and monitoring emergency response oil spills relative oil thickness
TexTOM - X-ray texture tomography as a tool to enable, multi-scale, in-situ imaging of the enthesis, a biological hinge between bone and tendon (TexTOM)

Framework: Horizon Europe — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Starting grants
Action type: ERC actions
Panel: Materials Engineering
Panel description: Advanced materials development: performance enhancement, modelling, large -scale preparation, modification, tailoring, optimisation, novel  and combined use of materials, etc.
Countries involved: France
Call: ERC STARTING GRANTS (ERC-2021-STG, 2021)
Start date: 2022-07-01
End date: 2027-06-30
Duration (months): 60.0
Total project cost (EUR): 1499694.0
EU funding (EUR): 1499694.0
Participants: 1
Project status: SIGNED

Hierarchical structures are signature elements of many biological and technical materials. The orientational distribution of their crystalline constituents (the crystallographic texture) is important for their mechanical properties. Resolving the local structure and orientation spatially while keeping a large field of view is an unsolved problem. I will solve this by introducing texture tomography, a new 3D x-ray diffraction imaging method, the core of the TexTOM project. It will enable to study the enthesis, the biological connection between tendon and bone, and by in-situ deformation and micromechanical modelling, couple its hierarchical structure with the mechanical behaviour. I will use the brilliance gain of 4th generation synchrotrons to develop texture tomography as a tool to image complex crystallographic textures in 3D and overcome the spatial resolution barriers of current approaches. I will develop the reconstruction approach for the crystallographic texture and use it to image the whole enthesis structure with 100nm spatial resolution and, with high energy x-rays, image the enthesis structure during in-situ tensile deformation with µm resolution at several load steps. The unique combination of 3D texture information and loading will allow to build a micromechanical enthesis model. The novelty lies in the structural 3D characterization of the enthesis under deformation. This is enabled by the development of texture tomography to reconstruct the 3D textures, which will be useful for many other scientific problems. I will build an accurate micromechanical enthesis model and this will shed light on the unknown load transfer mechanism in the enthesis on the nano- and crystal level. The flexible, open-source approach of TexTOM will ensure adaptation for new users and scientific problems. 4th generation synchrotrons will propel texture tomography to the forefront of (bio)materials science, revolutionizing our study of crystallographic textures.

Keywords: biomaterials characterization,x ray scattering,enthesis,biomechanics,crystallographic texture,micromechanical modelling
Highly Ionized Trapped 229-Thorium:A New Paradigm Towards a Nuclear Clock (HITHOR)

Framework: Horizon Europe — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Advanced grants
Action type: ERC actions
Panel: Fundamental Constituents of Matter
Panel description: Particle, nuclear, plasma, atomic, molecular, gas, and optical physics
Countries involved: Germany
Call: ERC ADVANCED GRANTS (ERC-2023-ADG, 2023)
Start date: 2024-07-01
End date: 2029-06-30
Duration (months): 60.0
Total project cost (EUR): 2498963.0
EU funding (EUR): 2498963.0
Participants: 1
Project status: SIGNED

Among the more than 3000 nuclides that are naturally occurring or that have been artificially synthesized, thorium-229 is truly exceptional. Its remarkably low excitation energy makes it the ideal candidate for nuclear frequency metrology, surpassing current atomic clocks. Such a "nuclear clock" opens doors to fundamental physics, testing time-variations of constants and exploring the enigma of dark matter. Within HITHOR a conceptually novel methodology towards such a clock is proposed, eventually leading to a no-electron “bare-nucleus” clock with trapped fully ionized 229Th90+ where all external perturbations from surrounding electrons are absent. Highly-ionized 229Th will be established as a distinctive laboratory for scientific explorations at the interface of atomic electrons and the nucleus. By adding a single electron to the nucleus, the unique effect of nuclear hyperfine mixing (NHM) is unlocked. Induced by the strong magnetic field of this one electron, a vast acceleration of the nuclear decay by up to a millionfold is triggered. Hence, for one-electron 229Th89+ laser excitation of the nucleus as well as the reemission of photons are each enhanced by up to this factor of a million. To date, laser excitation of the nucleus hasn’t been accomplished. NHM will help to overcome this obstacle and exploited as a booster towards 229Th laser excitation and a corresponding nuclear clock. HITHOR will be realized at the ion storage and trapping facilities at GSI in Darmstadt, the only laboratory worldwide where highly ionized 229Th can be synthesized in flight, decelerated, and finally trapped at rest. At GSI and its Helmholtz Institute Jena, I will bring together the extraordinary expertise of in-house scientists from the various disciplines required to tackle the challenges. Initial experiments will pave the path towards high-precision VUV laser spectroscopy of few- or no-electron 229Th, and ultimately towards a single-ion quantum logic nuclear clock.

Keywords: nuclear clock nuclear isomeric states heavy ion storage rings ion traps hyperfine quenching interface of atomic and nuclear physics highly charged ions thorium 229
Emerging Nanotools for Soft Matter Characterisation and Manipulation (NanoRAM)

Framework: Horizon Europe — Excellent Science
Programme: Marie Skłodowska-Curie Actions (MSCA)
Theme: Marie Skłodowska-Curie
Destination: Doctoral Networks
Action type: Marie Skłodowska-Curie actions
Panel: Information Science and Engineering
Countries involved: Denmark,Switzerland,Germany,Japan,United States,China,United Kingdom,Spain,France,Austria,Norway,Sweden
Call: MSCA Doctoral Networks 2022 (HORIZON-MSCA-2022-DN-01, 2022)
Start date: 2024-03-01
End date: 2028-02-29
Duration (months): 48.0
Total project cost (EUR): 2822896.8
EU funding (EUR): 2822896.8
Participants: 20
Project status: SIGNED

Advanced nanotools including atomic force microscopy, optical microscopy and correlative microscopy are enabling techniques for discoveries and knowledge generation in nanoscale science and technology. Many R&D efforts have been directed towards the performance improvement of such kinds of techniques for soft matter. However, the greatest challenge faced by these leading edge techniques is the realization of high spatiotemporal resolution, non-invasive, multi-scale and multi-dimensional imaging and manipulation. We therefore propose NanoRAM, a 10 ESR Marie Skłodowska Curie Action Doctoral Network by close collaboration between academic and industrial partners around the theme of innovative nanotools and their industrial applications. NanoRAM will train a new generation of ESRs in the development and application of newly developed manipulation and characterisation nanotools in soft matter research. ESRs will be cross-pollinated with concepts and skills in instrumentation and soft matter characterisation, in particular in fast nanomechanical spectroscopy, nano-robotics, correlative super-resolution nanoscopy, nano biomechanics and mechanotransduction. These skills are applied to reveal for the first time the fast, high resolution, multi-level and 3D information for single cell biomechanics and nanomedicine. Excellent training in new scientific and complementary skills, combined with international and intersectoral work experience, will instil an innovative, creative and entrepreneurial mind-set in ESRs, maximising economic benefits based on scientific discoveries. These specialised, highly trained ESRs will have greatly enhanced career prospects and qualifications for access to responsibility job positions in the private and public sectors. The ultimate goal of NanoRAM is to consolidate Europe as the world leader in innovative nanotool techniques and their emerging applications in soft matter fields such as biomechanics, mechanobiology, and nanomedicines.

Keywords: force nanoscopy,super resolution nanoscopy,nanorobotics,soft matter
Urban Phenology and Anthropogenic Climate Effects: getting the timing right in a warmer and more urban world (UrbanPACE)

Framework: Horizon Europe — Excellent Science
Programme: Marie Skłodowska-Curie Actions (MSCA)
Theme: Marie Skłodowska-Curie
Destination: Postdoctoral Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Environment and Geosciences
Countries involved: United Kingdom
Call: MSCA Postdoctoral Fellowships 2024 (HORIZON-MSCA-2024-PF-01, 2024)
Start date: 2026-07-01
End date: 2028-06-30
Duration (months): 24.0
Total project cost (EUR): 260347.92
EU funding (EUR): 260347.92
Participants: 1
Project status: SIGNED

Human-driven environmental change is affecting wildlife globally, with climate change and urbanisation having major impacts. For instance, warmer spring temperatures and urbanisation have been shown to independently advance the timing of recurring events in wild populations like leafing in trees, hatching in caterpillars, and egg laying in birds. Getting the timing right is critical for predator species like tits that rely on feeding prey resources like caterpillars to their chicks each spring. Tits can adjust the timing of their egg laying to match the hatching of their prey by responding to environmental cues like spring temperatures. In urban environments these cues may be highly modified and less reliable, which could negatively affect urban wildlife populations like tits if they can not track the timing of their prey. The UrbanPACE (Urban Phenology and Anthropogenic Climate Effects) research program will aim to establish whether urban tit populations across Europe adjust their egg laying to spring temperatures in the same way as nonurban populations, and evaluate the ability of urban populations to keep pace with climate change and further advances in spring timing. UrbanPACE will then determine whether spring timings are in sync between species in urban food webs. Using citizen science initiatives across the UK, I will explore whether climate change can increase mismatches between urban species by disrupting the timing of key events like leafing in trees and egg laying in tits. My results will provide new insights on how multiple processes of anthropogenic change interact to affect wildlife and evaluate whether urban populations are more vulnerable to a warming climate.

Keywords: urbanisation,urban evolutionary ecology,climate change,climate warming,phenology,lay date
Cocreate, Embrace (CulturalRoad)

Framework: Horizon Europe — Global Challenges and European Industrial Competitiveness
Programme: Climate, Energy and Mobility
Theme: Mobility
Destination: Safe, Resilient Transport and Smart Mobility services for passengers and goods
Action type: Research and Innovation actions
Partnership: CCAM (co-programmed)
Countries involved: Belgium,Denmark,Germany,Greece,Spain,Netherlands,Israel,Slovenia,United Kingdom,Switzerland
Call: Integrating European diversity in the design, development and implementation of CCAM solutions to support mobility equity (CCAM Partnership) (HORIZON-CL5-2023-D6-01, 2023)
Start date: 2024-05-01
End date: 2027-04-30
Duration (months): 36.0
Total project cost (EUR): 3510767.5
EU funding (EUR): 3510767.5
Participants: 18
Project status: SIGNED

CulturalRoad develops sustainable and population -wide accepted deployment plans for Cooperative, Connected, and Autonomous Mobility (CCAM) services by combining participatory planning with a novel Five-Pointed Star Rating system able to capture both cultural and geographical diversity. At its core, the project advocates for active engagement with local communities, gathering valuable insights on their mobility needs. Through participatory planning, stakeholders from various cultural backgrounds and geographical regions collaborate to share their mobility needs. This inclusive approach ensures that CCAM solutions are tailor-made to meet the distinct demands of each community, this is, it makes them vectors of a more equitable mobility.

Keywords: ccam,cultural issues,traffic management,infrastructure,safety,inclusivity and equity,sumps,numps