Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Visualizing Primase Initiating DNA Replication using NMR Spectroscopy (PRIMASE_NMR)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Switzerland
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2015, 2015)
Start date: 2016-03-01
End date: 2018-02-28
Duration (months): 24.0
Total project cost (EUR): 175419.6
EU funding (EUR): 175419.6
Participants: 1
Project status: CLOSED

The replication of DNA and therefore of the genetic information is realized at the molecular level in three steps: helicases open the DNA, primases initiate the replication and polymerases use this primer to duplicate the DNA strand. The precise mechanism of action of primases has up to now remain quite elusive and thus a better understanding of how primase works would be of great interest to fully understand the process of DNA replication. Primases are classed into two groups, bacterial and archeal/eukaryal, and some archeal primase have the particularity to carry their biological function without requiring the association in a larger molecular complex, making them an accessible target to Nuclear Magnetic Resonance (NMR) spectroscopy. In this project we propose to exploit the unique capacity of NMR spectroscopy for determining structure and dynamics of biomolecules in solution to investigate the ORF904 primase, free and in complex with its DNA template and the cofactors necessary for primer synthesis. We aim to characterize how this system assembles and to provide an atomic resolution picture of its mechanism of action, just before the creation of the first phosphodiester bound that initiate primer formation. We will also investigate the conformational changes occurring during the primer synthesis using Electron Paramagnetic Resonance (EPR) spectroscopy. Spectroscopic and computational innovative approaches will be developed to describe this complex dynamic system and complementary integrative structural biology will be used to support our findings. By this study we aim to provide an accurate description of a primase accomplishing its biological function and therefore significantly deepen our knowledge of DNA replication. This in turn could be used in cancer biology to develop new therapeutic approaches.

Keywords: primase,replication,protein dna complex,primer,molecular dynamics,molecular biology,conformation,dynamics,structure
Molecular mechanisms of photoprotection in plants. (EVERPHOT)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Reintegration panel
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Sweden
Call: Individual Fellowships (H2020-MSCA-IF-2018, 2018)
Start date: 2020-01-01
End date: 2021-12-31
Duration (months): 24.0
Total project cost (EUR): 203852.16
EU funding (EUR): 203852.16
Participants: 1
Project status: CLOSED

Photosynthesis is a biological process of primary importance, as it provides the energy that drives food, feedstock and biofuel production and mitigates climate change. Light in excess of photosynthetic capacity can be damaging, thus ways to protect against damage have evolved, including ways to minimize light absorption, detoxify reactive oxygen species generated by excess light, and dissipate excess absorbed light. Together, these processes are known as photoprotection. Despite the physiological importance of photoprotection, the molecular mechanisms that protect against light stress remain largely unknown, especially those that protect from prolonged light stress. The objective of the proposed research project is to solve molecular mechanisms of photoprotection in plants. My specific aims are to 1) investigate the function of known involved factors in sustained energy dissipation in the model plant Arabidopsis, 2) identify novel molecular players and 3) use an organism that is genetically adapted to cope with high light stress, the evergreen Norway spruce. The research will be carried out in my laboratory where genetics, biochemistry, biophysics and physiological approaches will be combined to address this problem. The research in my group will provide insights into fundamental mechanisms of light energy capture, utilization and dissipation and will lead to the identification of new targets for manipulation, key to increasing yields of energy and food crops.

Keywords: photosynthesis,photoprotection,non photochemical quenching,arabidopsis,spruce
Breakthrough Laser Technology for Dermatologic Healthcare (LIGHTSENSE)

Framework: Horizon 2020 — Industrial Leadership
Programme: Innovation in SMEs
Action type: SME instrument
Countries involved: United Kingdom
Call: SME instrument (H2020-SMEInst-2018-2020-1, 2018)
Start date: 2018-06-01
End date: 2018-11-30
Duration (months): 6.0
Total project cost (EUR): 71429.0
EU funding (EUR): 50000.0
Participants: 1
Project status: CLOSED

LightSense Technologies has developed and patented an innovative, non thermal ablative laser which constitutes a leap forward in laser technology with the potential to dramatically improve laser skincare and position Europe as a leader in medical and aesthetic applications. We are initially focused on tattoo removal because (1) the market is very large and growing, and well defined with clear patient needs, (2) the competitive offering is terrible, and (3) we have already developed meaningful intellectual property in this specific application with approved patents to support our work. We intend to quickly expand beyond tattoo removal, establishing industry-wide and academically-recognized laser solutions for many dermatology applications. We will begin by validating our business plan through the implementation of a Feasibility Study. The study will aim to better understand (A) technology performance in different laser-skin tissue studies, (B) commercial research with key stakeholders, and (C) financial strategy definition, as explained below. With this information, we will improve our laser tattoo removal treatment and refine our go-to- market strategy. LightSense Technologies forecasts ~€30M in revenues by 2021 (~€800M by 2030). Projections take in consideration an average price of € 2,850 per treatment, assuming a decrease thereafter.

Keywords: laser,aesthetic healthcare,tattoo removal
Upgrading Cyanines dyes: Non-Kekulé ketocyanines and related patterns (KETOCYAN)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Chemistry
Countries involved: France
Call: Individual Fellowships (H2020-MSCA-IF-2019, 2019)
Start date: 2020-08-01
End date: 2022-07-31
Duration (months): 24.0
Total project cost (EUR): 184707.84
EU funding (EUR): 184707.84
Participants: 1
Project status: CLOSED

The scientific goal of KETOCYAN is to renew classical nitrogen-containing polymethine dyes, so-called cyanines, and transform them into suitable tools for tackling the modern challenges of photoredox chemistry. The flagships for the proposed molecular patterns are unusual “non-Kelulé” diradicaloids. In principle their exotic topology must result in remarkable properties, including near-IR absorption, redox activity, enhanced photo-stability, etc. However, the unusual structures have also long been regarded as non-viable synthetic targets. This traditional belief has been challenged by recent reports and preliminary results of the host team. The project intend to capitalize on this paradigm shift, to develop synthetic methodologies, to study the novel organic functionalities, up to the design of original families of diradicaloid redox-active organic dyes. The fellow, Eder Tomas-Mendivil, is a seasoned synthetic chemist with solid track record and expertise in organometallic and organic chemistry, including the design and synthesis of unusual and/or reactive compounds. The host laboratory, the academic environment and the infrastructures at Université Grenoble Alpes will provide complementary training and experience, including in electrochemistry, photophysics, DFT calculation or study of radicals, but also in research management and communication. Overall this action will provide the fellow for outstanding career development opportunities, with a focus on academia.
Common mechanisms and pathways in Stroke and Alzheimer's disease. (CoSTREAM)

Framework: Horizon 2020 — Societal Challenges
Programme: Health, demographic change and wellbeing
Theme: Understanding health, wellbeing and disease
Action type: Research and Innovation actions
Countries involved: Germany,Austria,Switzerland,Sweden,France,France,France,Netherlands,Netherlands,United Kingdom,United Kingdom
Call: Understanding common mechanisms of diseases and their relevance in co-morbidities (H2020-PHC-2015-two-stage, 2015)
Start date: 2015-12-01
End date: 2021-05-31
Duration (months): 66.0
Total project cost (EUR): 5100372.5
EU funding (EUR): 4590185.0
Participants: 13
Project status: CLOSED

Common mechanisms and pathways in Stroke and Alzheimer's disease. It has long been recognized that stroke and (Alzheimer’s Disease) AD often co-occur and have an overlapping pathogenesis. As such, these two diseases are not considered fellow travelers, but rather partners in crime. This multidisciplinary consortium includes epidemiologists, geneticists, radiologists, neurologists with a longstanding track-record on the etiology of stroke and AD. This project aims to improve our understanding of the co-occurrence of stroke and AD. An essential concept of our proposal is that stroke and AD are sequential diseases that have overlapping pathyphysiological mechanisms in addition to shared risk factors. We will particularly focus on these common mechanisms and disentangle when and how these mechanisms diverge into causing either stroke, or AD, or both. Another important concept is that mechanisms under study will not only include the known pathways of ischemic vasculopathy and CAA, but we will explore and unravel novel mechanisms linking stroke and AD. We will do so by exploiting our vast international network in order to link various big datasets and by incorporating novel analytical strategies with emerging technologies in the field of genomics, metabolomics, and brain MR-imaging.

Keywords: stroke,alzheimer s disease,genomics,metabolomics,brain imaging,common mechanisms,pathophysiology,prediction,therapeutics
From haplotype to phenotype: a systems integration of allelic variation, chromatin state and 3D genome data (HAP-PHEN)

Framework: Horizon 2020 — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Starting grants
Action type: ERC actions
Panel: Integrative Biology: from Genes and Genomes to Systems
Panel description: Genetics, epigenetics, genomics and other ‘omics studies, bioinformatics, systems biology, genetic diseases, gene editing, innovative methods and modelling, ‘omics for personalised medicine
Countries involved: Netherlands
Call: ERC Starting Grant (ERC-2014-STG, 2014)
Start date: 2015-09-01
End date: 2020-08-31
Duration (months): 60.0
Total project cost (EUR): 1500000.0
EU funding (EUR): 1500000.0
Participants: 1
Project status: CLOSED

High-throughput sequencing methods are breaching the barrier of $1000 per genome. This means that it will become feasible to sequence the genomes of many individual and create a deep catalog of the bulk of human genetic variation. A great task will lie in assigning function to all this genetic variation. Genome wide association studies have already shown that 40% of all loci significantly associated with disease are found in intergenic, supposedly regulatory regions. One of the current challenges in human genetics is that variants that affect expression on a single allele cannot be directly linked, because only have genotype information, rather then haplotype information. The overarching aim of the project is to resolve haplotypes in order to identify genetic variants that affect gene expression. We will do this in three sub-projects. In the first main project we will use 3D genome information gathered from Hi-C experiments to haplotype the genomes of six lymphoblastoid cell lines. We will integrate these data with chromatin profiling and RNAseq data in order to build integrative models for the prediction of gene expression and the effect of genetic variation on gene expression. In the second project we will perform haplotyping the breast cancer genes BRCA1/2 in a large cohort of individuals that come from families with a high-risk of hereditary breast cancer. Allelic imbalance in BRCA1/2 expression levels are known to be associated with an increased risk for breast cancer. We will aim to find genetic variants that are associated with a decreased allelic expression of BRCA1/2 to improve breast cancer risk assessment. Finally, we will develop a novel tool to study 3D genome organization of single alleles, which will allow us to identify how individual alleles are organized in the nucleus and identify multi-way interactions (i.e. involving more than two genomic loci). With this we hope to better understand how complex 3D organization contributes to gene regulation.
COmbatting disorders of adaptive immunity with Systems MedICine (COSMIC)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Innovative Training Networks (ITN)-European Training Networks
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Germany,Spain,Germany,Italy,Spain,Sweden,Switzerland,Greece,Sweden,France,Netherlands,Netherlands,United Kingdom
Call: Innovative Training Networks (H2020-MSCA-ITN-2017, 2017)
Start date: 2018-01-01
End date: 2022-09-30
Duration (months): 57.0
Total project cost (EUR): 3605245.2
EU funding (EUR): 3605245.2
Participants: 18
Project status: CLOSED

Background: EU countries face large health challenges to combat chronic diseases. Recently, systems medicine has emerged as a promising discipline to accelerate the translation of basic research into applications for improved diagnostics and personalized treatment. Its power arises from the integration of laboratory and computational approaches crossing research disciplines and sectors to solve clinical questions. COSMIC delivers the next generation of leading, entrepreneurial, and innovative systems medicine professionals having expertise, skills, and experience to successfully combat complex human disorders. These professional will find excellent career opportunities. COSMIC focuses on B-cell neoplasia and rheumatoid arthritis, prototypical diseases originating from abnormal functioning of immune cells, often resulting in similar antigen specificities. COSMIC enables Early Stage Researchers to play a leading role in this exciting field. Approach: COSMIC develops and integrate experimental and computational approaches and establish a unique cross-fertilization between oncology and auto-immunity. In addition to transferable skills, the training program focuses on establishing a double expertise in laboratory and computational to address clinical questions. It involves a wide-range of stakeholders: (pre)clinical departments, companies, patient groups, students, and the general public. COSMIC will establish a link with the leading European EASyM and ISBE initiatives, and aims to harmonize systems medicine training throughout Europe by connecting to other EU (Marie Curie systems medicine) training initiatives. Impact: COSMIC (i) significantly improves ESR career perspectives (ii) leads to new public-private collaborations increasing competitiveness for companies; (iii) contributes to future oncology and immunology medical care; (iv) contributes to the EU systems medicine best practices.

Keywords: systems medicine,germinal centre,b cell receptor,rheumatoid arthritis,b cell neoplasia
A novel pathway for generation of building blocks for antibiotic biosynthesis (CaLiAT)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: United Kingdom
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2015, 2015)
Start date: 2016-09-01
End date: 2018-08-31
Duration (months): 24.0
Total project cost (EUR): 195454.8
EU funding (EUR): 195454.8
Participants: 1
Project status: CLOSED

The development and spread of antibiotic resistance in microorganisms is a major threat to both humans and animals and the search for new and improved drugs is of high importance. Natural products are experiencing a strong revival as leads in drug development, and biosynthetic engineering offers sustainable routes to new and potentially improved analogues. Finding new ways to make these rational changes should ensure that the European Research Area remains competitive in realising the potential of this technology. The aim of this project is to gain a detailed understanding of a newly-identified family of enzymes supplying unusual fatty acid building blocks for assembly-line biosynthesis of natural products; and to exploit these insights to develop more efficient strategies for targeted alteration of their structures. The novel precursor enzymes to be studied, a ligase and a biotin-dependent carboxylase, are in pathways to several polyketides produced by actinomycete bacteria. The project divides into three parts. First, additional examples of the new pathway will be sought by targetted whole-genome sequencing, as well as bio-informatic analysis of published sequence databases, and gene knockouts used to confirm the role of the pathway in providing polyketide extender units. Secondly, candidate ligases, carboxylases and the cognate acyltransferase domains that specifically recruit the unusual extender units will be studied in vitro for substrate specificity and for their ability to accept non-natural substrates bearing chemical functionality. Finally, a range of non-natural fatty acids will be fed as precursors to engineered strains designed to produce novel polyketide analogues. This approach should significantly expand the available pool of polyketide diversity. At the same time, the researcher will acquire high-level training in biochemical and chemical biology approaches that will help equip her for a leadership role in research.

Keywords: streptomyces,natural products,polyketide synthases,extender units,acyltransferase
Nanostructured carriers for improved cattle feed (NanoFEED)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Research and innovation staff exchange
Action type: Marie Skłodowska-Curie actions
Panel: Environment and Geosciences
Countries involved: Austria,Germany,Germany,Türkiye,Greece,Belgium,Slovakia,Czechia
Call: Research and Innovation Staff Exchange (H2020-MSCA-RISE-2017, 2017)
Start date: 2018-02-01
End date: 2023-04-30
Duration (months): 63.0
Total project cost (EUR): 733500.0
EU funding (EUR): 733500.0
Participants: 9
Project status: CLOSED

The project focuses on a highly actual problem of productivity and quality of livestock production. Livestock production is essential for a sustainable and self-sufficient manufacturing of wide range of products in food industry. One of the key strategies how to enhance competitiveness in the area of food industry is to increase the efficiency of stock raising. Nutritional deficiencies are key factors reducing the efficiency of stock raising, due to high prevalence of serious diseases. Maintaining optimal health of cattle is a key factor for increasing the efficiency of livestock. NanoFEED responds to this factors by nano/microencapsulation approach to advanced core/shell particles with pH sensitive properties.The proposed technology enables encapsulation of diverse active molecules and simplify the protection against degradation in rumen for wide range of active molecules. Nevertheless, due to encapsulation and improved delivery to small intestine, novel active molecules may be used to treat nutritional deficiencies. The process of encapsulation is based on green and biobased methods with minimal carbon footprint helping in development of sustainable bioeconomy. The results of NanoFEED will improve the cattle life expectation, quality of food and will promote more competitive livestock production. In order to achieve project goals, training network enabling efficient short term secondments of ER and ESR between academia and industry will be established. The cooperation between stakeholders helps in closing the gap between them and joint innovation to foster the current state of the art. NanoFEED will mediate it via networking, research, training, workshop, innovation and dissemination actions in form of short term secondments. The project fulfils the aim of RISE project and delivers significant social impact, increases knowledge based economy and creates long lasting cooperation strengthening the European research area.

Keywords: feed,cattle,calves,nanoparticles,microparticles,controlled release,core,shell,biopolymers,crosslinking,sustained release,ph sensitivity,chitosan,micronutrients,vitamins,algae,lactoferin
EcoBlade: Eco-efficient decommissioning of wind turbine blades through on-site material shredding and separation (EcoBlade)

Framework: Horizon 2020 — Societal Challenges
Programme: Secure, clean and efficient energy
Action type: SME instrument
Countries involved: Denmark
Call: Stimulating the innovation potential of SMEs for a low carbon energy system (H2020-SMEINST-1-2015, 2015)
Start date: 2015-07-01
End date: 2015-12-31
Duration (months): 6.0
Total project cost (EUR): 71429.0
EU funding (EUR): 50000.0
Participants: 1
Project status: CLOSED

Wind power plays a crucial role in Europe’s strategy towards a zero-carbon, clean energy-powered economy. While efforts have primarily focused on the development of wind turbine technology, it starts to become evident that the planning associated with the end-of-service life of these equipment has been vastly neglected. Rotor blades are a particularly challenging component, as there is uncertainty about how to get rid of them properly and safely. Furthermore, their sheer huge size imposes important constraints on the trucking requirements for their transportation, which translates in significant costs for decommissioning and disposal. EcoBlade presents a disruptive concept which tackles the cumbersome transportation of decommissioned large size rotor blades. Our mobile separation platform relies on a modular system optimized for blade shredding and material separation. It also opens the path towards profitable and economically sustainable value chains aiming at the revalorization of the disposed blade material. Since existing experience on blades’ decommissioning is still limited, disposal best practices are still to be defined. Therefore, the development of our scalable platform currently holds important economic risks, given the uncertainty on market acceptance. For this reason, Frandsen Industri firmly believes that a two-phase approach under EU-funding is the ideal scenario, in order to initially assess the market for concept feasibility before initiating the innovation project. Ecoblade will serve as a key enabler for future decommissioning of rotor blades, allowing to save more than 60 M€ in transportation costs for the disposed blades during the 2020-2030 period. Moreover, the successful implementation of EcoBlade will also significantly enhance the profitability of Frandsen Industri, as its successful implementation would return an expected turnover of nearly €5 million, 5 years post-project, corresponding to over €1.6 million profit to our company.

Keywords: material separation,blade decommissioning,transportation costs minimization,mobile shredder