Data-Gouv-ML/pcri-informations-sur-les-projets-laureats huggingface dataset:
Origin-dependent DNA replication visualised by Cryo-EM (DNA-Rep-EM)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Reintegration panel
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: United Kingdom
Call: Individual Fellowships (H2020-MSCA-IF-2020, 2020)
Start date: 2022-09-01
End date: 2024-08-31
Duration (months): 24.0
Total project cost (EUR): 224933.76
EU funding (EUR): 224933.76
Participants: 1
Project status: SIGNED

Genome duplication is essential for cell proliferation. Errors in the mechanisms that control DNA replication can cause genomic instability and lead to the development of genetic diseases and cancer. In vitro reconstitution of DNA replication with purified yeast proteins has helped uncover important mechanisms of DNA replication. How changes in protein structure regulates function during key events in origin activation and replisome progression, such as melting of the DNA duplex upon CMG helicase assembly, or the mode of binding of Pol alpha during primer synthesis remain unknown. To date, structural studies have focused on imaging artificially isolated replication complexes using simplified DNA substrates to understand DNA unwinding and replisome architecture. To truly understand the mechanisms that control DNA replication, future structural studies must not only visualise isolated complexes, but also reconstituted reactions. To address this issue, I will integrate single-particle cryo electron-microscopy (cryo-EM) with sophisticated biochemical approaches to image origin-dependent DNA replication reactions in vitro on native DNA substrates. To do so, I will establish short origin-containing DNA substrates that permit loading of a single bidirectional replication fork, allowing large numbers of protein bound origins to be visualised within a single field of view. Initially, I will investigate how the structure of duplex DNA changes upon CMG formation during origin activation. Next, I will examine the molecular mechanisms of primer synthesis after origin activation using both cryo-EM and in vitro DNA replication reactions. Finally, I will capture and image synthesising intact replisomes at near atomic resolution. By visualising entire DNA replication reactions instead of isolated replication complexes at high resolution, we will gain a deeper understanding of the molecular mechanisms that permit the eukaryotic replisome to function during genome duplication.

Keywords: dna replication,replisome,cryo em,in vitro reconstitution,helicase,polymerase
Highly Informative Drug Screening by Overcoming NMR Restrictions (HiSCORE)

Framework: Horizon 2020 — Excellent Science
Programme: European Research Council (ERC)
Theme: European research council
Destination: Synergy grants
Action type: ERC actions
Countries involved: Germany,Switzerland,France,Netherlands
Call: ERC Synergy Grant (ERC-2020-SyG, 2020)
Start date: 2021-05-01
End date: 2027-04-30
Duration (months): 72.0
Total project cost (EUR): 13992719.0
EU funding (EUR): 13992719.0
Participants: 4
Project status: SIGNED

The need for drug screening with increasingly higher throughput is dictated both by the increasing number of drug targets made available through genomics and the increasing number of chemical molecules generated through combinatorial chemistry. Merely Boolean high-throughput screening techniques today can scan large compound libraries, but the ever increasing throughput has not translated into a significant increase in late-phase drug candidates. HiSCORE presents a synergistic approach to high-throughput, high-information drug screening that builds on the complementary skills of four laboratories supported by two external experts of drug screening: (i) Research and build innovative magnetic resonance instrumentation (Kentgens, IMM/RU) that can provide small, hyperpolarized solid samples on a seconds timescale, transfer and dissolve or liquefy these samples with minimum dilution, and acquire multiple high-resolution NMR spectra of the liquefied samples in parallel (Meier, IBG/KIT), using complementary contrast-enhancement methods, in up to 1000 massively parallelized microfluidic detectors (Korvink, IMT/KIT). (ii) Use this instrumentation for binding assays and measure the dissociation constants in the nano to micromolar range, and determine kinetic rates of the association and dissociation for a large number of complexes of putative drug compounds and protein targets (Bodenhausen, ENS) (iii) Use this instrumentation for functional assays, in particular for systems that comprise multiple enzyme steps with intermediate products, and to determine the efficacy of potential inhibitors, while fully exploiting the rich information that can be obtained by fluorine-19 NMR. (iv) Use this instrumentation for metabonomic assays to observe the metabolism of the compounds in cultures of living cells in view of identifying potentially toxic side-products. The contrast between compounds that bind to targets and those that fail to bind will be boosted by exploiting long-lived states

Keywords: dissociation constants,kinetic rates,enzyme inhibition,high throughput screening,microfluidics,supercritical co2,parallel detection,dynamic nuclear polarization,para hydrogen
Europe-wide Connected Transport (Simacan LINK)

Framework: Horizon 2020 — Industrial Leadership
Programme: Innovation in SMEs
Action type: SME instrument
Countries involved: Netherlands
Call: SME instrument (H2020-SMEInst-2018-2020-2, 2019)
Start date: 2019-12-01
End date: 2021-11-30
Duration (months): 24.0
Total project cost (EUR): 3316875.0
EU funding (EUR): 2321812.5
Participants: 1
Project status: CLOSED

Shippers and transport companies face an increasingly challenging society. Individuals and businesses expect to get goods delivered faster, more flexible, on time and at low cost. At the same time, the sector is confronted with increasing emission restrictions, congestion, a lack of truck drivers and low profits despite a strong growth in volume driven by e-commerce. Realtime collaboration between shippers and carriers and digital data sharing holds the key to solving these challenges in a growing market. Current available digital tooling however is often optimized for dedicated parts of the supply chain, lacking interoperability to integrate and communicate within the heterogeneous environment of transport companies, retailers, third parties and customers. Simacan solves these problems by developing an intelligent data exchange and collaboration platform. Transport operation data is enriched with high quality traffic information, enabling automated, realtime management by exception and communication during transport execution. It allows for integration with existing logistics systems like planning systems, onboard units, driver apps and freight sensors and creates a joint view and intervention options for all stakeholders in the transport execution process. The Simacan solution revolutionized the Dutch market for transport management of fast-moving consumer goods. It gained market traction with leading retailers and transport companies reaching a market share of 55% in the Dutch Grocery Store supply chain and 80% in Dutch Home Grocery Shopping in the last four years. This project will bring the Simacan platform from a successful, national product, to a commercially scalable Europe-wide Connected Transport platform worth the name Simacan LINK. With the support of several leading retailers, transport companies and technology partners, Simacan aims to value its research and development over the past years and bring Europe’s freight management to the next level.

Keywords: real time transport management,delivery,retail,digital collaboration,e cmr,algorithms,saas,supply chain,routing,network,distribution,e commerce
Boosting the Innovative Potential of Romanian SMEs (Ro-Boost Inno SMEs)

Framework: Horizon 2020 — Industrial Leadership
Programme: Innovation in SMEs
Action type: Specific Grant agreement
Countries involved: Romania,Romania,Romania
Call: Enhancing the innovation management capacity of SMEs (H2020-Adhoc-2014-20, 2014)
Start date: 2015-01-01
End date: 2016-12-31
Duration (months): 24.0
Total project cost (EUR): 79647.4
EU funding (EUR): 79647.26
Participants: 6
Project status: CLOSED

In the context of innovation-divided Europe and scarce consultancy in RDI, Macro Region 4 Romania is facing its peak of natural development and moving towards its "turning point". The core members of the Ro-Boost SMEs consortium are aware that it is time for smart action in order to achieve the goal of reducing the innovation divide among companies. Based on the vision stated in the Ro-Boost SMEs Implementation Strategy, the core members of the consortium jointly elaborated the project "Ro-Boost Inno SMEs" that is aiming to consolidate innovative businesses - rooted into the Smart Specialisation Strategy - and to expand their position within the sector global value chains. As being ambitious, this goal can be achieved by "surgical" interventions, bringing added value to SMEs and innovative sectors, using an innovative delivery system and by setting up a vibrant community of innovators. The proposal is built around relevant institutions at Macro Region 4 level, using in-depth competences of our experts to create success regarding the indicators, satisfaction among clients, impact at macro regional level and innovation culture within business environment.

Keywords: smart specialisation,innovation management,innovative products,horizon 2020
Metabolic interactions in oceanic photosymbioses (MINOTAUR)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Environment and Geosciences
Countries involved: Germany
Call: Marie Skłodowska-Curie Individual Fellowships (IF-EF) (H2020-MSCA-IF-2014, 2014)
Start date: 2016-01-01
End date: 2017-12-31
Duration (months): 24.0
Total project cost (EUR): 171460.8
EU funding (EUR): 171460.8
Participants: 1
Project status: CLOSED

Symbiosis between heterotrophic hosts and microalgae (photosymbiosis) is a widespread and ecologically important phenomenon in the oceanic plankton. Symbiotic organisms like radiolarians (unicellular eukaryotes) are key players in marine biogeochemical cycles by contributing to predation and primary production. While knowledge of the diversity of symbiotic partners has improved in recent years, metabolic interactions remain poorly understood. This project will explore the metabolic basis of planktonic photosymbiosis, with radiolarians as an ecologically relevant model, to understand the functioning of the partnership and its contribution to elemental cycling in the pelagic ecosystem. An original and cutting-edge single-cell approach involving stable isotopes and high-resolution chemical imaging techniques (e.g. ToF-SIMS and nanoSIMS) will be used to visualize the elemental and isotopic composition of intact radiolarian symbioses at the subcellular level, and to quantify the assimilation and transfer of nutrients between partners in different experimental conditions. The same approach will be applied on cultured free-living symbionts to determine the degree of host control over symbiont metabolism. In order to develop a holistic view of metabolic interactions, bioinformatic analyses will identify key metabolic genes and pathways from available transcriptomes of radiolarians. The exceptional microscopy facility and expertise in isotope biogeochemistry at the host institution is unique in Europe. Given the interdisciplinary nature of the project consortium, the potential for exchanging new knowledge and skills is very high. This project pushes back the boundaries of marine biological research and represents a significant step in my personal development towards my long-term research ambition to merge knowledge on biodiversity and physiology into ecological studies to better understand the functioning of aquatic ecosystems and their responses to anthropogenic pressures.

Keywords: single cell chemical imaging,secondary ion mass spectrometry sims imaging,marine eukaryotic plankton,micro algae,photosymbiosis,transcriptome mining,stable isotope labeling
Host switching pathogens, infectious outbreaks and zoonosis; a Marie Sklodowska-Curie Training Network (HONOURs)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Innovative Training Networks (ITN)-European Training Networks
Action type: Marie Skłodowska-Curie actions
Panel: Life Sciences
Countries involved: Germany,Germany,Spain,Germany,Switzerland,Belgium,Netherlands,Netherlands,Netherlands,United Kingdom
Call: Innovative Training Networks (H2020-MSCA-ITN-2016, 2016)
Start date: 2017-04-01
End date: 2021-03-31
Duration (months): 48.0
Total project cost (EUR): 3860395.56
EU funding (EUR): 3860395.56
Participants: 13
Project status: CLOSED

The ongoing increase of the human population accompanied by growing numbers of livestock to feed this population, as well as human invasion into natural habitats of wild animals makes humans progressively vulnerable to animal pathogens. Global trade as well as climate changes can contribute to pathogen transmission, e.g. through import of infected vectors or expansion of habitats for arthropod vectors such as mosquitoes and midges. Examples for this include recent European outbreaks of the arthropod-borne Dengue, West Nile, Schmallenberg and Usutu viruses in humans, ruminants and birds. Infectious disease outbreaks in animals are often unexpected, but we should be prepared for immediate identification of the causing agent and the evaluation of its pathogenic potential for animals and humans. Preparedness needs expertise in many areas. At surveillance sites early detection and identification of viruses involved in outbreaks is crucial. Screening of different animal species and humans who have been in close contact with (diseased) animals for known or yet undiscovered viruses will also help to be prepared for future outbreaks. Novel viruses in these populations will have to be evaluated for their pathogenicity in susceptibility studies. The HONOURs network will generate new health and scientific knowledge with the aim to locate, identify, characterize, contain, and control infections that are caused by zoonotic agents. The aim of HONOURs is to provide top-quality cross-disciplinary and supra-sectorial training to 15 promising young researchers at the interface of veterinary and human health, virology, biostatistics, and pathogen discovery. These early stage researchers will become “preparedness- experts”.

Keywords: zoonosis,preparedness
On the ModElling of micro-robots in the Gut: a non-smooth dynamics Approach (OMEGA)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Information Science and Engineering
Countries involved: United Kingdom
Call: Individual Fellowships (H2020-MSCA-IF-2020, 2020)
Start date: 2021-10-01
End date: 2023-09-30
Duration (months): 24.0
Total project cost (EUR): 212933.76
EU funding (EUR): 212933.76
Participants: 1
Project status: SIGNED

Detection of bowel cancer is currently performed by visual inspection of the colonic mucosa during endoscopy, which is less reliable for small-sized lesions that are not easily visualised. If they are not detected and removed at an early stage, there is a chance that they may become cancerous. This project seeks to develop a new mathematical tool for analysing the sensing capability of micro-robots to aid the detection of hard-to-visualise bowel lesions. Micro-robots experiencing vibrations, frictions, and impacts, known as non-smooth dynamical systems, exhibit a rich variety of different long-term behaviours co-existing for a given set of parameters, which is referred to as multi-stability or co-existing attractors. When the robot moves in the colon and encounters a lesion, some particular attractor may dominate its dynamics, while the other co-existing attractors could fade away due to the tissue’s mechanical properties associated with different stages of malignant transformation. This significant change in multi-stability can be utilised to distinguish between healthy and abnormal tissues. The fellow proposes to use for the first time robot’s multi-stability through the development of state-of-the-art numerical techniques to analyse such robot-lesion correlation, and produce a suite of computational analysis and advanced control methods for cancer detection and staging. In the long term, this work will initiate a new modality for bowel cancer screening, delivering an efficient minimally invasive procedure for patients. The unique research approach of this fellowship, a joint effort of numerical and experimental studies, will be hosted by Dr Yang Liu from the University of Exeter with the secondment supervisor, Prof. Bradley Nelson from ETH Zurich, and the consulting gastroenterologist, Dr Shyam Prasad, from the Royal Devon and Exeter NHS Foundation Trust.

Keywords: piecewise linear dynamical systems,non smooth dynamical systems,ddes,micro robot,multistability,bowel cancer,early detection
Enhancing aesthetic reception of contemporary music through active tempo control with physiological monitoring (EAR Stretch)

Framework: Horizon 2020 — Excellent Science
Programme: Marie-Sklodowska-Curie Actions
Theme: Marie Skłodowska-Curie
Destination: Individual Fellowships (IF)-Standard European Fellowships
Action type: Marie Skłodowska-Curie actions
Panel: Social Sciences and Humanities
Countries involved: France
Call: Individual Fellowships (H2020-MSCA-IF-2020, 2020)
Start date: 2021-08-15
End date: 2023-08-14
Duration (months): 24.0
Total project cost (EUR): 196707.84
EU funding (EUR): 196707.84
Participants: 1
Project status: SIGNED

Complex contemporary music (CM) is the crucible for musical invention that exemplifies human creativity. It forms the lifeblood of musical cultural evolution, but can be alienating to the uninitiated. In EAR Stretch, we propose to help non-specialized listeners gain a deeper appreciation for CM through embodied interaction with temporal expression of the music. When communicating music, skilled performers often help listeners make sense of the music through expressive prosodic inflections of timing and tempo. In familiar music, listeners can often recognize the pulse and tap a foot along to the expressed beat. But in CM, listeners’ internalization of the beat and subsequent embodied reactions may be limited. The embodied cognition that comes from physically engaging with time—including organization of intrinsic brain activity and changes in perception—may be lacking in CM listening, contributing to poor reception. We hypothesize that empowering listeners to physically control the temporal expression of CM will lead to improved reception of the music as measured by neural and physiological changes and aesthetic evaluation of the music. Our main objective is to determine the impact of motor-based temporal engagement with CM on the listener-performer’s reception of the music. Acoustic- and complexity-matched CM compositions will be created specially for a psychophysiological study comparing normal listening to interactive listening with tapping-based temporal control. Objective measures, neural and physiological responses, will probe the underlying causes of subjective aesthetic evaluations and elucidate the roles of arousal, attention, and entrainment in these sources. Positive impact of interactive, embodied engagement with CM will transform the CM landscape, inspire the redesign of concert experiences, promote social inclusion, and propel CM into the musical vernacular, inciting cultural and intellectual development.

Keywords: music perception and cognition,temporal perception
Plastic nanocomposite insulation material enabling reliable integration of renewables and DC storage technologies in the AC energy grid (GRIDABLE)

Framework: Horizon 2020 — Industrial Leadership
Programme: Nanotechnologies, Advanced Materials, Advanced Manufacturing and Processing, and Biotechnology
Theme: Advanced materials
Action type: Innovation actions
Countries involved: Italy,Germany,Germany,Italy,Italy,Finland,Slovakia,Switzerland,Finland,France,Netherlands,United Kingdom
Call: Advanced materials enabling the integration of storage technologies in the electricity grid (H2020-NMBP-2016-two-stage, 2016)
Start date: 2017-01-01
End date: 2021-06-30
Duration (months): 54.0
Total project cost (EUR): 7076524.1
EU funding (EUR): 5574156.25
Participants: 14
Project status: CLOSED

In the GRIDABLE project we will introduce novel thermoplastic polymer composite materials to enhance performance of essential components of smart grid infrastructure. We have proven in laboratory scale that the polypropylene-silica nanocomposite brings considerable improvements especially in dielectric breakdown strength. When applied as insulator in high-voltage direct current (HVDC) cable and in DC capacitors, the composite will bring significant enhancement at device level compared to the state-of-the-art. Cost and physical size of the capacitors will be reduced. Thermoplastic composite will enable cost effective production of cables by extrusion. Additionally, enhanced dielectric properties will increase HVDC transfer capacity. This will result in more efficient use of energy and materials. In the GRIDABLE project we will up-scale production of novel dielectric nanocomposite for electrical insulation applications. We will transfer material’s high performance from laboratory scale to pre-production scale. This will be proven by relevant demonstrators and prototypes. The technical advances gained with novel dielectric material will facilitate to enhance power supply reliability. The new level of dielectric characteristics will help to manage volatility of the grid considering variety of power sources. Thus grid efficiency can be increased. The new HVDC cables will allow efficient electricity transfer over very long distances, e.g., from remote low-carbon power plants. This will also ease utilisation of distributed and intermittent renewable energy sources.

Keywords: polypropylene,silica,nanocomposite,dielectric,electrical insulator,dielectric strength