Projet ANR-07-BLAN-0171 – DIAMS : Desorption/Ionisation laser on self-Assembled Monolayer Surfaces
Programme : Blanc (BLANC) 2007
Année de financement : 2007
Coordinateur du projet : CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE BRETAGNE ET PAYS- DE-LA-LOIRE (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DELEGATION REGIONALE BRETAGNE ET PAYS- DE-LA-LOIRE)
Partenaires : Centre national de la recherche scientifique; Université d'Angers
Montant : 275000.0 euros
Date de début : 2007
Durée : 36 mois
Résumé : "The project acronym is DIAMS for Desorption/Ionisation on Self-Assembled Monolayer Surfaces. It refers to the surface modification by Self-Assembled Monolayers (SAM) in order to use their specific properties through mass spectrometry analysis under Laser Desorption/Ionization (LDI). -  -  The main objectives are: -  *	Understanding the physical processes of LDI on organic self-assembled monolayer surfaces, -  *	Establishing molecular engineering rules in order to design and elaborate organic SAM adapted to LDI. -  *	Developing an original and alternative method to MALDI technique. -  -  Therefore, this project associates fundamental as well as applied researches while its interdisciplinary quality implies skills in organic chemistry and analytical chemistry. -  -  Indeed, two research teams, gathering a complementary knowledge in organic chemistry, physical chemistry and analytical chemistry, are associated to develop this project: -  *	The CIMMA who have a recognized expertise in electrochemistry, mass spectrometry and in the development of self-assembled monolayer devices, -  *	The SONAS who is recognized for its expertise in organic synthesis, analytical chemistry and phytochemistry. -  -  The project will be built in a four-step way: -  -  A.	Deployment of molecular engineering to synthesize target molecules dedicated to LDI process. The wettability (lipophily, hydrophily) of devices will be a very important criterion in order to adapt surfaces to analytes. -  B.	Elaboration and characterization of materials by simple and reliable techniques. In this purpose, the use of a redox material should allow a simple characterization by electrochemistry in terms of organization, stability and surface coverage. A fluorescent material would be also an alternative. The use of STM/AFM microscopies will be necessary to complete this characterization. -  C.	Understanding and modelization of all stages, from the photonic energy absorption to the desorption/ionization process according to the nature of the studied analyte and the surfaces implemented. -  D.	Validation of the analytical potentialities of this novel method through the study of standard samples in order to: (i): establish a relation between the repeatability/reproducibility of measurements and the quality of worked out materials, (ii): determine the sensitivity of the method in a broad range of m/z ratio, (iii): conclude on the applicability of this technique in quantifications. Finally, the method will be applied to phytochemical analysis during the characterization of secondary metabolites exhibiting therapeutical interest such as antifongic, cytotoxic or antioxidant properties. We plan to use here some original samples from crude vegetal extracts (e. g.: EtOAc extracts of endemic Neocaledonian Clusiaceous species), which chemical composition will have been determined previously, by means of conventional analytical techniques (CLHP, LC-MS, mono and two-dimensional NMR...). -  -  Steps A and B will be developed jointly by the members of CIMMA and SONAS, whereas steps C and D will be more specific to the CIMMA or to the SONAS team, respectively. -  -  The feasibility of the project was already established and published (Sanguinet, Lionel ; Alévêque, Olivier ; Blanchard, Philippe ; Dias, Marylène ; Levillain, Eric ; Rondeau, David. Desorption/Ionization on self-Assembled Monolayer Surface (DIAMS). Journal of Mass Spectrometry (2006), 41, 830). -  "                                                                                                                                                                                                                                                                                                 ...