Projet ANR-07-BLAN-0084 – INMEMBRANE : Membrane protein complexes: a new approach
Programme : Blanc (BLANC) 2007
Année de financement : 2007
Coordinateur du projet : Monsieur James STURGIS (CNRS DELEGATION REGIONALE PROVENCE ET CORSE)
Partenaires : Centre national de la recherche scientifique
Montant : 319200.0 euros
Date de début : 2007
Durée : 48 mois
Résumé : Bacteria, in common with other cells, are separated from their environment by a selectively permeable membrane. Through this membrane must pass all the material and information that travels between the cell and the environment  including information about the possibility of colonisation, food for the bacteria to grow, virulence factors released by the bacteria and antibiotics targeting the cell. The membrane can be considered as the wall of the cell with the lipid parts acting as a barrier and the membrane proteins orchestrating the transfer of information and material. In this context it is not surprising that membrane associated proteins represent between a third and a half of the genome and proteome of most cells. However, for several technical reasons our knowledge of membrane protein interaction networks is relatively sparse. Thus, systematic studies frequently ignore membrane proteins. In this project, we propose to develop several universal methods specifically adapted for the description and analysis of membrane protein interaction networks. We will start in Escherichia coli, and then extend these methods to other systems including the opportunistic pathogen Pseudomonas aeruginosa, and eukaryote membranes. -  -  This multidisciplinary project relies on the complementary expertises derived from four research groups in the laboratory: Prof. Sturgis in the biophysics of membranes and membrane proteins; Dr. Bouveret in microbiology, genetic screens for protein-protein interactions and tandem affinity purification (TAP); Dr Filloux in Pseudomonas aeruginosa and Dr. Lloubes in analysis of outer membrane organisation and inter-membrane junctions. This project thus depends on the development of a project specific transversal research group through the collaboration of researchers and students from each of the existing groups within the laboratory on a common project. This is why we have made this application as a single research group headed by Prof. Sturgis.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             ...