Flow Cytometric Analysis of Fish Sperm Quality: A Case Study of Black Sea Turbot
Journal: Trudy VNIRO
Year: 2025

Objective: the aim of this study was to develop a method for evaluating fish sperm quality based on the mitochondrial status of spermatozoa, which is linked to their functional motility. Methods: to assess sperm quality of sexually mature Black Sea turbot males of different ages from a spawning population, flow cytometric analysis with preliminary staining of live sperm samples using the fluorescent dye rhodamine 123, which characterizes the membrane potential of spermatozoa mitochondria, was ap