Perspectives of RNAi, CUAD and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice
Journal: Preprints.org
Year: 2025

Pest management has entered a new era with the emergence of three innovative antisense technologies: RNAi, CUAD, and CRISPR/Cas. These technologies, which operate through sequence-specific nucleic acid duplex formation and guided nuclease activity, offer unprecedented potential for targeted pest control. While RNA-guided systems such as RNAi and CRISPR/Cas were initially discovered in non-insect models as fundamental biological mechanisms (primarily in antiviral defense), the DNA-guided CUAD sys