Perspectives of RNAi, CUADb and CRISPR/Cas as Innovative Antisense Technologies for Insect Pest Control: From Discovery to Practice
Journal: Preprints.org
Year: 2025

Pest management has entered a new era with the emergence of three innovative antisense technologies: RNA interference (RNAi), contact unmodified antisense DNA biotechnology (CUADb), and CRISPR/Cas systems. These approaches function through sequence-specific nucleic acid duplex formation and guided nuclease activity, offering unprecedented precision for targeted pest control. While RNA-guided systems such as RNAi and CRISPR/Cas were originally discovered in non-insect models as fundamental biolog