INDUCED MUTAGENESIS IN CULTURE OF ISOLATED TISSUES OF SAINTPAULIA
Journal: Acta Biologica Sibirica
Year: 2016

The paper studied the effects of colchicine on isolated tissue regenerative capacity of violet saintpaulia (Blue Dragon). We determined the optimal dose of the mutagen exposure time and to preserve cell viability and maximize the number of plants regenerated with modified genotype. We used leave fragments (5 × 5 mm) for cell lines. Isolated leaf explants were cultured on MS medium supplemented with benzylaminopurine (BAP) and α-naphthaleneacetic acid at a concentration of 3 and 4 uM respectively