Creation of source for marking and mapping of frost resistance QTL in winter barley
Journal: Plant varieties studying and protection
Year: 2025

Purpose. Creation the sets of recombinant-inbred lines for marking and mapping of frost resistance QTL in winter barley. Methods. Hybridization, artificial vernalization, growing plants under long-day conditions in phytotron light chambers and on a plot of land under natural daylight duration condition, DNA isolation with use CTAB-buffer, spectrophotometric determination of DNA concentration, polymerase chain reaction with direct primers, agarose and polyacrylamide gels electrophoresis, method o