Determination of protodioscin in tribulus terrestris by reversed-phase high-performance liquid chromatography
Journal: E3S Web of Conferences
Year: 2021

In this study, high performance liquid chromatography (HPLC) was used to analyze the protodioscin content in Tribulus terrestris samples. They were separated by C18 chromatography column, gradient with 0.1 % phosphoric acid solution and acetonitrile was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 45 °C, and the detection wavelength was 203 nm. The results showed that the protodioscin content has a good linear correlation (r&gt;0.999) within the range of