Detection of Malassezia Fungi in Microplankton and Fishes in the Severnaya Bay, Sevastopol (Russia)
Journal: Микология и фитопатология / Mycology and Phytopathology
Year: 2025

In this study, the microplankton and mycobiota of fish in Severnaya Bay, Sevastopol, was assessed using real-time PCR (RT-PCR). Microplankton samples were collected using a Biber-2 sequential filtration setup, and microbiota samples were obtained from the surface of fishes (Trachurus mediterraneus and Spicara smaris). DNA was extracted by alkaline lysis during high-temperature incubation and analyzed for the presence of 13 species of fungal pathogens from the genera Candida, Malassezia, Saccharo