Useful Molecular and Cytogenetic Approaches in Population Genetics Studies of Pine Species
Journal: Genetics.
Year: 2024

In the last decade, we characterised Portuguese populations of Pinus sylvestris L., Pinus nigra Arnold and Pinus pinaster Aiton by using different molecular and cytogenetic approaches. DNA markers helped assess intra- and inter-population genetic variability, extrapolation of phylogenies, provenances and/or infraspecific taxonomy. Quantitative real-time polymerase chain reaction (qRT-PCR), Classical Cytogenetics techniques and/or Comet assay were valuable to assign origins, infraspecific taxa or