title: Adam Gilchrist

text: Team success came in the Mercantile Mutual Cup, where the Warriors won by eight wickets against Queensland in the March 1997 final; Gilchrist was not required to bat.


title: Adam Gilchrist

text: The 1997–98 season ended with Gilchrist top of the dismissals chart for the fourth season in a row with an improved batting average of 47.66, despite playing in only six of the ten qualifying Shield matches due to his becoming a regular member of the national limited overs team. Gilchrist registered his maiden–first-class double century with an unbeaten 203 against South Australia early in the season, before returning late in the season after his international commitments were over. He added 109 against Victoria, and played in the Sheffield Shield final victory over Tasmania, although he scored only eight. There was disappointment for the team in the Mercantile Mutual Cup, losing the semi-final to Queensland.


title: Adam Gilchrist

text: The following season saw Gilchrist's domestic appearances diminish due to his international commitments: he made only a single appearance in the Mercantile Mutual Cup, but still managed to help Western Australia defend the Sheffield Shield, scoring a century in the qualifying rounds.


title: Adam Gilchrist

text: Gilchrist's regular selection for Australia meant that he was rarely available for domestic selection after he became the Test wicket-keeper in late-1999; between 1999 and 2005, he made only seven first-class appearances for his state.


title: Adam Gilchrist

text: Gilchrist played a total of six seasons in the Indian Premier League (IPL), the major Twenty20 franchise league in India, three for Deccan Chargers and three for Kings XI Punjab. He was signed by Deccan for the 2008 season, the inaugural season of the competition, having been purchased for US$700,000 in the player auction a few months after his retirement from international cricket.


title: Adam Gilchrist

text: Before the fourth season of the IPL Gilchrist was bought at the 2011 player auction by Kings XI Punjab for US$900,000 and was, again, appointed as captain, taking over from Kumar Sangakkara who had moved to Deccan. In March 2012 he was named player-coach of the side for the following season, replacing his friend and former Australia teammate Michael Bevan, whose contract as head coach was not renewed. After the team failed to make the play-offs, Gilchrist speculated that he may choose to retire from cricket.


title: Adam Gilchrist

text: Following the appointment of Darren Lehmann, who had previously worked with Gilchrist at Deccan, as head coach, Gilchrist chose to play one more IPL season for Kings XI, once again as captain. In May 2013, Gilchrist announced his retirement from the IPL. A planned appearance in the first season of the Caribbean Premier League had to be cancelled after an ankle injury and the match proved to be Gilchrist's last in top-class cricket. In that fixture, Gilchrist took the wicket of Harbhajan Singh, from his one and only ball he ever bowled in a T20 match.


title: Adam Gilchrist

text: Over his six seasons in the IPL Gilchrist played a total of 82 matches, 48 for Deccan and 34 for Kings XI. He scored more than 2,000 runs, including two centuries. He was also the first cricketer to score 1000 runs in IPL.


title: Adam Gilchrist

text: Gilchrist signed a short-term contract in November 2009 to play Twenty20 cricket for Middlesex County Cricket Club in England during 2010. He was appointed interim captain of the T20 side on 11 June following the sudden resignation of Shaun Udal. He played in seven matches for the side during the 2010 Twenty20 Cup, scoring 212 runs at an average of 30.28, including a century made against Kent at Canterbury, as well as captaining the county against the touring Australians in a one-day match ahead of their ODI series against England. The season was Gilchrist's only one spent playing county cricket.


title: Adam Gilchrist

text: Gilchrist was called up for the Australian One Day International (ODI) team in 1996, his debut coming against South Africa at Faridabad on 25 October 1996 as the 129th Australian ODI cap, after an injury to incumbent Ian Healy. While not particularly impressive with the bat on his debut, scoring 18 before being bowled by Allan Donald, Gilchrist took his first catch as an international wicketkeeper, Hansie Cronje departing for a golden duck from the bowling of Paul Reiffel. He was run out for a duck in his only other ODI on the tour. Healy resumed his place during the 1996–97 season. Gilchrist replaced Healy for the first two ODIs in the 1997 Australian tour of South Africa, after Healy was suspended for dissent. When Healy returned Gilchrist maintained his position in the team as a specialist batsman after Mark Waugh sustained a hand injury.


title: Adam Gilchrist

text: It was during this series that Gilchrist made his first ODI half-century, with an innings of 77 in Durban. He totalled 127 runs at 31.75 for the series. Gilchrist went on to play in the Texaco Trophy later in 1997 in the 3–0 series loss against England, scoring 53 and 33 in two innings.


title: Adam Gilchrist

text: At the start of the 1997–98 Australian season, Healy and captain Mark Taylor were omitted from the ODI squad as the Australian selectors opted for Gilchrist and Michael di Venuto. Gilchrist's elevation was made possible by a change in policy by selectors, who announced that selection for ODI and Test teams would be separate, with Test and ODI specialists selected accordingly, while Healy remained the preferred Test wicket-keeper. This came after Australia failed to qualify for the previous season's ODI triangular series final for the first time in 17 years. The new team was initially unconvincing, losing all four round robin matches against South Africa in the 1997–98 Carlton & United Series, with multiple players filling Taylor's role as Mark Waugh's opening partner without success. Gilchrist also struggled batting in the lower order at number seven, the conventional wicket-keeper's batting position, scoring 148 runs at 24.66 in the eight qualifying matches.


title: Adam Gilchrist

text: In the first final against South Africa at the Melbourne Cricket Ground Gilchrist was selected as Waugh's opening partner. In a particularly poor start to the new combination, Waugh was run out after a mix-up with Gilchrist. However, in the second final, Gilchrist struck his maiden ODI century, spearheading Australia's successful run chase at the Sydney Cricket Ground, securing his position as an opening batsman. Australia won the third final to claim the title.


title: Adam Gilchrist

text: Touring New Zealand in February 1998, Gilchrist topped the Australia averages with 200 runs at 50.00, including a match-winning 118 in the first match. He also effected his first ODI stumping, the wicket of Nathan Astle in the Second ODI in Wellington. Australia then played two triangular tournaments in Asia. Gilchrist struggled in India, scoring 86 runs at 17.20. He went on to play in the Coca-Cola Cup in Sharjah in April 1998, a triangular tournament between Australia, India and New Zealand. Australia finished runners-up in the tournament, with Gilchrist taking nine dismissals as wicketkeeper and averaging 37.13 with the bat.


title: Adam Gilchrist

text: Gilchrist won a silver medal at the 1998 Commonwealth Games in Kuala Lumpur, the only time cricket has been in the Commonwealth Games. The matches did not have ODI status, and after winning their first four fixtures, Australia lost the final to South Africa, Gilchrist making 15. He then scored 103 and ended with 190 runs at 63.33 as Australia took a rare 3–0 whitewash on Pakistani soil.


title: Adam Gilchrist

text: Gilchrist was in fine form ahead of the 1999 Cricket World Cup with a productive individual performance in the Carlton & United Series in January and February 1999 against Sri Lanka and England. He finished with 525 runs at a batting average of 43.75 with two centuries—both against Sri Lanka—and a fifty, and a total of 27 dismissals in 12 matches. His 131 helped Australia set a successful run-chase at the SCG, and he followed this with 154 at the MCG.


title: Adam Gilchrist

text: The 1999 tour of the West Indies was Australia's last campaign before the World Cup and continued to prove Gilchrist's ability as a wicketkeeper-batsman. Gilchrist, with a batting average of 28.71 and a strike rate of nearly 90.00, and seven fielding dismissals in a seven-match series which ended 3–3 with one tie.


title: Adam Gilchrist

text: Gilchrist played in every match of Australia's successful World Cup campaign, but struggled at first, with scores of 6, 14 and 0 in the first three matches against Scotland, New Zealand and Pakistan. Australia lost the latter two matches and had to avoid defeat for six consecutive matches to reach the final. Gilchrist's quick-fire 63 runs in 39 balls against Bangladesh helped the Australians into the Super Six stage of the tournament, which was secured with a win over the West Indies, although Gilchrist made only 21. Gilchrist continued to struggle in the Super Six phase, scoring 31, 10 and 5 against India, Zimbabwe and South Africa. Australia won all three matches, the last in the final over, to scrape into the semifinals. Gilchrist made only 20 in the semifinal against South Africa, but completed the final act of the match. With the scores tied, South Africa were going for the winning run when Gilchrist broke the stumps to complete the run out of Allan Donald; the match was tied, and Australia proceeded to the final as they had won the group stage match against South Africa. Gilchrist's 54 in the final helped secure Australia's first world title since 1987 with an eight wicket victory over Pakistan. It was a happy ending for Gilchrist, who had struggled through the tournament, with 237 runs at 21.54.


title: Adam Gilchrist

text: Success at the World Cup was followed by a defeat by Sri Lanka in the final of the Aiwa Cup in August 1999. Gilchrist was the most successful batsman and wicket-keeper of the tournament, with 231 runs at 46.20. While the Test players battled against Sri Lanka, Gilchrist led Australia A in a limited overs series against India A in Los Angeles. He then scored 60 runs at 20.00 as the Australians completed a 3–0 whitewash of Zimbabwe in October.


title: Adam Gilchrist

text: Gilchrist made his Test match debut in the First Test against Pakistan at the Gabba in Brisbane in November 1999 becoming the 381st Australian Test cricketer. He replaced Healy, who was dropped after a run of poor form, despite the incumbent's entreaties to the selectors to allow him a farewell game in front of his home crowd. Gilchrist's entry into the Test arena coincided with a dramatic rise in Australia's fortunes. Up to this point, they had played eight Tests in 1999, winning and losing three.


title: Adam Gilchrist

text: Gilchrist's icy reception at the Gabba did not faze him; he took five catches, stumped Azhar Mahmood off Shane Warne's bowling and scored a rapid 81, mostly in partnership with ODI partner Waugh, in a match that Australia won comfortably by ten wickets. In his second Test match he made an unbeaten 149 to help guide Australia to victory in a game that looked well beyond their reach. Australia were struggling at 5/126 in pursuit of 369 for victory as he joined his Western Australian teammate, Justin Langer, but the pair put on a record-breaking partnership of 238 to seal an Australian win. Gilchrist continued his strong run throughout his debut Test season, and ended the summer with 485 runs at 69.28 in six matches, three each against Pakistan and India, adding two fifties against the latter.


title: Adam Gilchrist

text: Gilchrist was moderately successful in the following ODIs, the Carlton & United Series; Australia defeated Pakistan 2–0 in a best-of-three final. Gilchrist scored 272 runs at 27.20; his best effort was 92 in a 152-run victory over India on Australia Day. Gilchrist then scored 251 runs at 41.66 in the ODIs during a tour of New Zealand. The highlight was a 128 in Christchurch that propelled Australia to a score of 6/349. Gilchrist was named man of the match in two of the games.


title: Adam Gilchrist

text: In the Third Test against New Zealand in 2000, Gilchrist recorded the third best Test performance ever by a wicketkeeper, and the best by an Australian, taking ten catches in the match. Although Gilchrist's batting was modest, yielding 144 runs at 36.00, Australia took a 3–0 clean sweep. In two home and away ODI series against South Africa, Gilchrist had a quiet time, scoring 170 runs at 26.66. South Africa won three of the six matches, with one tie.


title: Adam Gilchrist

text: Later that year, he was handed the vice-captaincy of the Australian team in place of Shane Warne, who had been plagued by a number of off-field controversies, including an altercation with some teenage boys, and a sex scandal with a British nurse.


title: Adam Gilchrist

text: The 2000–01 season saw a West Indian touring party and Gilchrist warmed up with consecutive first-class centuries for Western Australia. Captaining his Test team for the first time in place of the injured Steve Waugh in the Third Test in Adelaide. Gilchrist scored only 9 and 10 not out, but a ten-wicket haul from Colin Miller resulted in a hard-fought five-wicket victory for Australia. Gilchrist described the match as "the proudest moment of my career". Waugh resumed the captaincy on his return to the team for the Fourth and Fifth Tests, with the series finishing in a 5–0 whitewash. Gilchrist scored 241 runs at 48.20 with two fifties. In the ensuing ODI tournament, Gilchrist scored 326 runs at 36.22 with a top-score of 98 as the Australians won all ten matches.


title: Adam Gilchrist

text: Up to this point, Gilchrist had played in 14 Tests, all in Australasia, and all of which had been won. Australia's run of 15 consecutive Test wins faced a steep challenge on the tour of India, where they had not won a Test series since 1969–70.


title: Adam Gilchrist

text: Australia's streak looked in danger during the First Test in Mumbai when they fell to 5/99 in reply to India's 171 when Gilchrist came to the crease. He counterattacked savagely, scoring 122 in just 112 balls, and featuring in a 197-run partnership with Matthew Hayden in only 32 overs. This swung the momentum back to Australia, who reached 349. Gilchrist took six catches and was named Man of the Match in a ten wicket victory, extending the world record run to 16.


title: Adam Gilchrist

text: Gilchrist's form dipped momentarily, with a rare "king pair" (two golden ducks in the same match) in the Second Test in Kolkata and just two runs in his two innings in Chennai. He was out LBW four consecutive times in the last two Tests, three of these to Harbhajan Singh, who took 32 wickets in the series to end Australia's run by inflicting a 2–1 series loss. His one-day form remained strong, with 172 runs at 43.00 in the ODI series in India, as Australia bounced back to win the series 3–2. During this series he captained the ODI team for the first time, winning all three of the matches under his captaincy.


title: Adam Gilchrist

text: Gilchrist played a pivotal role in the 2001 Ashes series which Australia won 4–1, with 340 runs at a batting average of 68.00 and 26 dismissals in the five-match series.


title: Adam Gilchrist

text: Gilchrist warmed up by putting his ODI struggles on English soil in 1999 behind him, scoring 248 runs at 49.60 in the triangular tournament preceding the Tests, scoring an unbeaten 76 in the final win over Pakistan.


title: Adam Gilchrist

text: Gilchrist put the disappointment of India behind him in the First Test at Edgbaston, scoring 152 from only 143 balls. The allowed Australia to reach 576 in only 545 minutes, and set up an innings victory that set the tone for the series. Gilchrist then added 90 in the eight-wicket win in the Second Test at Lord's, before turning the tide in the Third Test at Trent Bridge. Australia slumped to 7/105 in reply to the hosts' 185, but Gilchrist's 54 took the tourists to 190 before a seven-wicket win resulted in the retention of the Ashes.


title: Adam Gilchrist

text: Gilchrist captained the team in the Fourth Test at Headingley after an injury to Steve Waugh. After persistent rain interruptions, Gilchrist declared with Australia four down at tea on the fourth day, leaving England with a target of 315, which, despite losing two early wickets, they reached with six wickets to spare, (Mark Butcher scoring an unbeaten 173, including 24 boundaries). Gilchrist failed to pass 25 in the last two Tests, but it had been a productive season; he scored centuries in both of Australia's county matches.


title: Adam Gilchrist

text: Two home series followed in the 2001–02 season, a fully drawn (0–0) three match series against New Zealand and a whitewash over South Africa 3–0. Gilchrist scored 118 in the First Test against New Zealand and an unbeaten 83 in the Third Test in Perth as the Australians held on for a draw with three wickets intact. However, Gilchrist did little in the triumph over South Africa, failing to pass 35. He ended the summer Tests with 353 runs at 50.42.


title: Adam Gilchrist

text: In the ensuing ODIs, Gilchrist scored only 97 runs at 16.16. The Australian selectors sought to accommodate Hayden, who had been successful as a Test opener, into the ODI team by rotating him with Gilchrist and Waugh, but this appeared to unsettle the team. With a newly fragile top order, Australia failed to qualify for the finals, and the Waugh brothers were dropped from the team, ending Gilchrist's four-year partnership with Mark. Ricky Ponting was promoted to the captaincy ahead of vice captain Gilchrist.


title: Adam Gilchrist

text: The Australians then toured South Africa the next month and it was during the First Test in Johannesburg that Gilchrist broke the record for the fastest double century in Tests on 23 February, requiring 212 balls for the feat. This was eight balls quicker than Ian Botham's innings against India at The Oval in 1982. He ended unbeaten on 204, having featured in a partnership of 317 with Damien Martyn at a run rate of 5.5. South Africa were demoralised and lost by an innings after being forced to follow on. The record lasted only one month, however, with New Zealand's Nathan Astle taking 59 balls less to reach the milestone during an innings in March 2002.


title: Adam Gilchrist

text: In the Second Test at Cape Town, Gilchrist struck 138 from 108 balls to set up a first innings lead and eventual four-wicket win. He then top-scored with 91 in the Third Test, and although Australia lost the match, Gilchrist ended the series with an astonishing 473 at 157.66 from just 474 balls, in addition to 14 dismissals.


title: Adam Gilchrist

text: Gilchrist captained the ODI team, once again for a single match, against Kenya in Nairobi during the PSO Tri-Nation Tournament. Despite Australia's unbeaten run in the competition, the final, against Pakistan was abandoned due to rain, so the teams shared the trophy. During the six middle months of 2002, Gilchrist played in 18 ODIs, scoring 562 runs at 31.22, including a century, recovering from his slump.


title: Adam Gilchrist

text: After scoring 122 runs at 40.66 in the 3–0 Test series clean sweep over Pakistan in the United Arab Emirates, Gilchrist went on to help the Australians retain The Ashes 4–1 in 2002–03, playing in all five matches of the series, finishing with 330 runs at 55.50 and taking 25 dismissals as wicket-keeper. After scoring fifties in the first two Tests, Gilchrist scored a counter-attacking 133 from 121 balls in the Fifth Test at the SCG, but was unable to prevent Australia's only loss of the series.


title: Adam Gilchrist

text: From the time of his debut up to the 2003 World Cup, Gilchrist's played in 40 Tests in series. With the exception of the 2001 tour of India, when he averaged 24.80 (he made 124 runs in the series; 122 of them came in one innings), his performances with the bat were such that he was described at the time as the "finest batsman-wicketkeeper to have graced the game". At one point in March 2002, Gilchrist's Test average was over 60; the second-highest for any established player in Test history, and he topped the ICC Test batting rankings in May 2002.


title: Adam Gilchrist

text: Gilchrist warmed up for the World Cup in South Africa by scoring 310 runs at 44.28 in the triangular tournament in Australia against England and Sri Lanka. His performances over the past year were recognised with the Allan Border Medal.


title: Adam Gilchrist

text: Gilchrist played in all but one of the matches in Australia's successful defence of their World Cup title; he was rested for the group match against the Netherlands. He finished the tournament with 408 runs at an average of 40.80 at a strike rate of 105. He scored four half-centuries, and was run out against Sri Lanka in the Super Six stage just a single run short of a century. In the semi-final, he scored 22 before being caught off an inside-edge onto pad off the bowling of Aravinda de Silva. The umpire gave no reaction, however Gilchrist walked off the pitch after a moment's pause. In 2009 it was described as an "astonishing moment" drawing criticism from England's Angus Fraser, who "objected to him being canonised simply for not cheating", and from others who "thought that he walked almost by accident; that having played his shot he overbalanced in the direction of the pavilion." His actions nevertheless drew praise from the majority. In the final, India elected to field first and Gilchrist hammered 57 from 48 balls, featuring in a century opening stand with Hayden to seize the initiative. This laid the foundation for Australia's 2/359 and a crushing 125-run win, ending an unbeaten campaign. Gilchrist was also the competition's most successful wicketkeeper, making 21 dismissals.


title: Adam Gilchrist

text: Success in the World Cup was followed up by a tour of the West Indies where Gilchrist was part of a side that won both the ODI and Test series. He scored 282 runs at 70.50 with one century in the four Tests, and 212 runs at 35.33 in the ODIs. The Australians then defeated a touring Bangladeshi cricket team in short series in both forms of the game. Gilchrist was only sporadically required with the bat.


title: Adam Gilchrist

text: After scoring his first Test century at his home ground in Perth, an unbeaten 113 against Zimbabwe, Gilchrist's Test form dipped again during the 2003–04 season, with only 120 runs coming in the next 10 innings, during the home series against India (drawn 1–1) and the away series in Sri Lanka (won 3–0). However, he returned to form in the Second Test Kandy, scoring a quickfire 144 in the second innings to set up a 27-run win after Australia conceded a 91-run first innings lead.


title: Adam Gilchrist

text: However, he maintained high standards in ODIs during this period, including 111 against India in Bangalore, 172 against Zimbabwe, just one run short of Mark Waugh's Australian record, and two further half-centuries in the VB Series in Australia. His success in One-day cricket was underlined by his rise to the top of the ICC ODI batting rankings in February 2004. However, he was unable to maintain this form on the 2004 tours of Sri Lanka, Zimbabwe and the Champions Trophy in England, accumulating 253 runs at 28.11 in 11 innings.


title: Adam Gilchrist

text: Gilchrist then scored 115 runs at 28.75 in two Tests at home to Sri Lanka in mid-2004, and captained in the First Test win in Darwin with Ponting absent. Australia won the series 1–0.


title: Adam Gilchrist

text: A 104 in the First Test against India in October 2004 proved to be a false renaissance; he scored only 104 runs in the remaining seven innings on the Indian tour and 139 runs in eight ODI innings towards the end of the 2004–05 season, which formed the lowest average period of Gilchrist's career until 2007. He took the captaincy of the Test team once again, in place of the injured Ricky Ponting, and led the Australian side to a historic 2–1 series victory in India, a feat last achieved in 1969. Ponting recovered to lead the team in the Fourth Test, Australia's only loss.


title: Adam Gilchrist

text: Gilchrist returned to form when New Zealand toured Australia at the start of southern hemisphere season. He scored 126 and 50 in the 2–0 Test series clean sweep and scored fifties in both ODIs. He then scored 230 runs at 76.66 in three Tests against Pakistan, including a rapid 113 in the Third Test at the SCG as Australia won all five Tests during the summer. He made it three successive Test centuries with 121 and 162 in the first two Tests on the tour of New Zealand, before ending with an unbeaten 60 in the Third Test; he totalled 343 runs at 114.33 for the series. His ODI form in the early part of 2005 remained moderate, with 308 runs at 28.00 during the southern summer.


title: Adam Gilchrist

text: Gilchrist was in strong form ahead of the Tests, scoring 393 runs at 49.13 in the ODIs in England. The highlight was the 121 not out in the final game of the one-day NatWest Series, Gilchrist being awarded the man-of-the-match award. However, he performed poorly in the five Tests, with 204 runs at 25.50. Just as in India in 2001, Australia lost 2–1.


title: Adam Gilchrist

text: Australia and Gilchrist returned to form after the Ashes in the series against the ICC World XI. Gilchrist scored 45, 103 and 32 as Australia swept the ODIs 3–0, and top-scored with 94 in the first innings of the one-off Test, which Australia won. However, this did not transfer into the regular international matches. In six home Tests against the West Indies and South Africa in 2005–06, Gilchrist managed only 190 runs at 23.75, but Australia was unhindered, winning 3–0 and 2–0 respectively.


title: Adam Gilchrist

text: His one-day form also began to suffer, scoring only 11 runs in three ODIs in New Zealand and 13 in the first two matches of the VB Series. He was rested for two games and returned to form against Sri Lanka on 29 January 2006 on his home ground, the WACA, hitting 116 runs off 105 balls to lead Australia to victory. He continued in this vein with the fastest ever century by an Australian in just 67 balls against Sri Lanka at the Gabba, ending with 122 as Australia won the deciding third final by nine wickets. After a slow start, he ended the series with 432 runs at 48.00.


title: Adam Gilchrist

text: The purple patch ended on the tour of South Africa and then Bangladesh. He scored 206 runs at 29.42 in five Tests and 248 runs at 35.42 in eight ODIs, inflated by a 144 in the First Test against Bangladesh. Despite this, Australia won all five Tests. Gilchrist scored 130 runs at 26.00, including a 92 against the West Indies as Australia won the 2006 Champions Trophy in India.


title: Adam Gilchrist

text: On 16 December 2006, during the Third Ashes Test at the WACA, Gilchrist scored a century in 57 balls, including twelve fours and four sixes, which at the time was the second fastest recorded Test century. At 97 runs from 54 balls, Gilchrist needed three runs from the next delivery to better Viv Richards' record set in 1986. The ball delivered by Matthew Hoggard was wide and Gilchrist was unable to score from it.


title: Adam Gilchrist

text: He later claimed that the "batting pyrotechnics" had been the result of a miscommunication between Michael Clarke and him with the Australian captain Ricky Ponting; Gilchrist had actually been told "not" to score quick runs with a view to declaring the innings.


title: Adam Gilchrist

text: He ended the 2006–07 Ashes with a century and two fifties, totalling 229 runs at 45.80 at a strike rate of over 100 as Australia regained the Ashes with a 5–0 whitewash. It was an inconsistent series; aside from three scores mentioned, Gilchrist failed to pass one in his other three innings. Between Ashes series, Gilchrist had averaged only 25 with one Test century.


title: Adam Gilchrist

text: However, both he and Australia suffered a surprising string of poor results in the 2006–07 Commonwealth Bank Series, Gilchrist managing an average of only 22.20 during the tournament. Australia won seven of their eight qualifying matches, but England won with two finals victories over the Australians. Gilchrist scored 60 and 61 in the first two matches but did not pass 30 thereafter. He was then rested for Australia's winless three-match ODI tour of New Zealand, before his selection for the 2007 Cricket World Cup. Having previously indicated that it was highly likely that he would retire after the 2007 World Cup, he then stated a desire to play on afterwards.


title: Adam Gilchrist

text: Gilchrist and Australia started their 2007 World Cup campaign by winning all three of their matches in Group A, against Scotland, the Netherlands and South Africa. Australia won all seven of their matches in the Super8 stage with little difficulty—the margins of victory exceeded 80 runs or six wickets in every instance. They topped the table and thus qualifying for a semi-final rematch against fourth-placed South Africa. Gilchrist opened the Australian batting in each match, taking a pinch-hitting role in the opening powerplays. Initially successful in the group matches, scoring 46, 57 and 42, he failed in the first Super8 match against West Indies (7), but bounced back to score a second half-century (59 not out) in a ten-wicket victory against Bangladesh in a match drastically shortened due to rain. After a run of middling scores, he failed again in the final Super8 match against New Zealand.


title: Adam Gilchrist

text: As a batsman, Gilchrist was dismissed for a single run in the semi-final against South Africa, despite which Australia won by seven wickets. Gilchrist opened the batting against Sri Lanka in the final. This was Gilchrist's third successive World Cup final, and the third time he scored at least 50 runs in a World Cup final and he went on to make his only ever century in a world cup match (his previous best World Cup score having been 99 against Sri Lanka in the 2003 tournament). Gilchrist went on to score 149 runs off 104 balls with thirteen fours and eight sixes, the highest individual score in a World Cup final, eclipsing his captain Ricky Ponting's score of 140 in the 2003 final. Australia won and he was named the man of the match. Subsequently there has been some controversy over Gilchrist's use of a squash ball inside his glove during this innings.


title: Adam Gilchrist

text: The MCC stated that Gilchrist had not acted against the laws or the spirit of the game, since there is no restriction against the external or internal form of batting gloves.


title: Adam Gilchrist

text: In September 2007, Gilchrist played in the inaugural World Twenty20. He scored 169 runs at 33.80 as Australia were knocked out by India in the semifinals. Gilchrist then scored 208 runs at 34.66 as Australia took an away ODI series against India 4–2. In November, Gilchrist's peers voted him the greatest Australian ODI cricketer ever, for which he was awarded an honour at an ACA function before Australia's second Test against Sri Lanka. He was only required to bat once in the Tests, and made 67 not out as Australia swept Sri Lanka aside 2–0.


title: Adam Gilchrist

text: On 26 January 2008 during the 4th and final Test of the 2007–08 series against India, Gilchrist announced that he would retire from international cricket at the end of the season. A back injury kept Ricky Ponting off the field for sections of the Indian's second innings, resulting in Gilchrist captaining the team for part of the final two days of his Test cricket career. India batted out the match for a draw, so Gilchrist's 14 in the first innings was his final Test innings; he took his 379th and final catch when Virender Sehwag was caught behind. Gilchrist had scored only 150 runs at 21.42 in his final Test series.


title: Adam Gilchrist

text: John Buchanan, who coached Australia during most of Gilchrist's international career, predicted that Gilchrist's retirement would have more impact than the previous year's retirements of Damien Martyn, Glenn McGrath, Shane Warne and Justin Langer and Australian Prime Minister Kevin Rudd asked Gilchrist to reconsider. Gilchrist later revealed that he chose to retire after dropping VVS Laxman during the first innings, and realising that he had lost his "competitive edge." He played out the summer's ODI series, before ending in disappointment when India beat Australia 2–0 in the 2007–08 Commonwealth Bank Series finals. Gilchrist managed only seven and two in the finals. His highlight of the series was his scoring 118 and being named Man of the Match in his final match at his adopted home in Perth on 15 February 2008, against Sri Lanka. He ended his final series with 322 runs at 32.20.


title: Adam Gilchrist

text: Gilchrist's attacking batting was a key part of Australia's one-day success, as he usually opened the batting. He was a part of the successful 1999, 2003 and 2007 Cricket World Cup campaigns. Gilchrist's Test batting average in the upper 40s is unusually high for a wicket-keeper. He retired from Test cricket at 45th on the all–time list of highest batting averages. At the end of his Test career he had established a Test strike-rate of 82 runs per hundred balls, at the time the third highest since balls were recorded in full. His combination of attack and consistency create one of the most dynamic world cricketers ever, playing shots to all areas of the field with uncommon timing. He was second on the all-time list of most sixes in Tests at 100 with only Brendon McCullum ahead of him with 107. Gilchrist's skills as a wicket-keeper were sometimes questioned; some claimed that he was the best keeper in Australia whilst others that Victorian wicket-keeper Darren Berry was the best Australian wicket-keeper of the 1990s and early 2000s. Gilchrist attributed his batting techniques from early training with his father, where he would defend shots, sometimes only gripping the bat with his top (right) hand, and would end a session to simply play attacking shots with tennis balls to end on a positive and fun note. He also adopted a naturally high grip where both hands were closer to the end of the handle for more top hand control.


title: Adam Gilchrist

text: Gilchrist successfully kept wicket for fast bowlers Glenn McGrath and Brett Lee for most of his international career. His partnerships with McGrath and Lee are second and fourth respectively in both test and ODI history for the number of wickets taken. With Alec Stewart and Mark Boucher, he shares the record for most catches (6) by a wicketkeeper in a ODI match, having achieved this feat five times. In 2007 he took six dismissals and scored a half century in the same ODI for the second time; he remains the only player to do so even once. At Old Trafford in August 2005, he passed Alec Stewart's world record of 4,540 runs as a Test wicketkeeper, and at his retirement in 2008, he was the most successful ODI wicket-keeper with 472 dismissals (417 catches and 55 stumpings), more than 80 dismissals ahead of his closest rival, Mark Boucher. This record was surpassed seven years later by Kumar Sangakkara.


title: Adam Gilchrist

text: It is unusual for professional batsmen to "walk"; that is, to agree that they have been dismissed and leave the field of play without waiting for (or contrary to) an umpire's decision. Gilchrist reignited this debate by walking during a high-profile match, the 2003 World Cup semi-final against Sri Lanka, after the umpire ruled him to be not out. He has since proclaimed himself to be "a walker", or a batsman who will consistently walk, and has done so on numerous occasions. On one occasion against Bangladesh, Gilchrist walked but TV replays failed to suggest any contact between his bat and the ball. Without such contact, he could not have been caught out.


title: Adam Gilchrist

text: Gilchrist's actions have sparked debate amongst current and former players and umpires. Ricky Ponting has declared on several occasions that he is not a walker but will leave it to each player to decide whether they wish to walk or not. While no other Australian top-order batsmen have expressly declared themselves to be walkers, lower-order batsmen Jason Gillespie and Michael Kasprowicz both walked during Test matches in India in 2004. In 2004, New Zealand captain Stephen Fleming accused Gilchrist of conducting a "walking crusade" when Craig McMillan refused to walk after Gilchrist had caught him off an edge from the bowling of Jason Gillespie in the First Test in Brisbane. After the appeal was turned down by the umpire, who did not hear the edge, Gilchrist goaded McMillan about the edge, and McMillan's angry response was picked up by the stump microphone: "...not everyone is walking, Gilly ... not everyone has to walk, mate...". The taunt was arguably effective, however, as McMillan, perhaps distracted, missed the next ball and was given out leg before wicket. Gilchrist said in his autobiography that he had "zero support in the team" for his stance and that he felt that the topic made the dressing room uncomfortable. He added that he "felt isolated" and "silently accused of betraying the team. Implicitly I was made to feel selfish, as if I was walking for the sake of my own clean image, thereby making everyone else look dishonest."


title: Adam Gilchrist

text: Gilchrist has been noted for his emotional outbursts on the cricket field, and has been fined multiple times for dissent against umpiring decisions. In January 2006, he was fined 40% of his match fee in an ODI against South Africa. In another instance, in early 2004 in Sri Lanka, Gilchrist audibly argued with umpire Peter Manuel after batting partner Andrew Symonds was given out. After the argument concluded, Manuel consulted umpiring partner Billy Bowden and reversed his decision, recalling Symonds to the crease. Gilchrist was also reprimanded by the Australian Cricket Board for publicly questioning the legality of Muttiah Muralitharan's bowling action in 2002, as his comments were found to be in breach of the clause in the player code of conduct relating to "detrimental public comment".


title: Adam Gilchrist

text: During the 2003 World Cup, Gilchrist accused Pakistani wicket-keeper Rashid Latif of making a racist remark towards him while the latter was batting in their group match. Latif who was cleared by match referee Clive Lloyd, threatened to sue Gilchrist for this claim.


title: Adam Gilchrist

text: Gilchrist was one of five Wisden Cricketers of the Year for 2002, and Australia's One-day International Player of the Year in 2003 and 2004. He was awarded the Allan Border Medal in 2003, and was the only Australian cricketer who was a current player at the time to have been named in "Richie Benaud's Greatest XI" in 2004. He was selected in the ICC World XI for the charity series against the ACC Asian XI, 2004–05, was voted as "World's Scariest Batsman" in a poll of international bowlers, and was named as wicket-keeper and opening batsman in Australia's "greatest ever ODI team." In a poll of over ten thousand people hosted in 2007 by ESPNcricinfo, he was voted the ninth greatest all-rounder of the last one hundred years. A panel of prominent cricket writers selected him in Australia's all-time best XI for ESPNcricinfo.


title: Adam Gilchrist

text: In 2010, Gilchrist was made a Member of the Order of Australia (AM) for his services to cricket and the community. He was inducted into the Sport Australia Hall of Fame in 2012. On 9-December-2013, ICC announced that they had inducted Gilchrist in the prestigious ICC Hall of Fame. He was named an Australia Post Legend of Cricket in 2021.


title: Adam Gilchrist

text: Gilchrist's autobiography "True Colours", published in 2008, was the subject of much controversy. Gilchrist questioned the integrity of leading Indian batsman Sachin Tendulkar in relation to the evidence he presented in the Monkeygate dispute, which was about allegations of racism against Harbhajan Singh. The autobiography said that Tendulkar told the first hearing that he could not hear what Harbhajan said to Andrew Symonds; Gilchrist said that he was "certain he ""Tendulkar"" was telling the truth" because he was "a fair way away". Gilchrist then questioned why Tendulkar then agreed with Harbhajan's claim at the second hearing that the exchange was an obscenity, and concluded that the process was "a joke". He also raised questions over Tendulkar's sportsmanship and said he was "hard to find for a changing-room handshake after we have beaten India".


title: Adam Gilchrist

text: There was a backlash in India, which forced Gilchrist to clarify his position. Gilchrist later insisted that he did not accuse Tendulkar of lying in his testimony. He also denied calling the Indian a "bad sport" in regards to the handshake issue. Tendulkar responded by saying that "those remarks came from someone who doesn't know me enough. I think he made loose statements...I reminded him that I was the first person to shake hands after the Sydney defeat." The autobiography also blamed the ICC for allowing Sri Lankan cricketer Muralitharan to bowl; Gilchrist believes that ICC changed the throwing law to legitimise a bowling action that he regards as illegitimate. The law change was described as "a load of horse crap. That's rubbish." Gilchrist claimed that Muralitharan threw the ball and alleged that the ICC protected him because Sri Lankan cricket authorities portrayed any criticism of the bowler's legitimacy as racism and a witch-hunt conducted by whites. In response to these comments, former Sri Lankan captain Marvan Atapattu said that by questioning the credentials of players like Muralitharan and Tendulkar, Gilchrist had done no good to his own reputation.


title: Adam Gilchrist

text: Outside cricket, Gilchrist is an ambassador for the charity World Vision in India, a country in which he is popular due to his cricketing achievements, and sponsors a boy whose father has died. He was approached in early 2005 by the US baseball franchise, the Boston Red Sox, with a view to him playing for them when his cricket career ended. However, he was selected for the 2007 Cricket World Cup and announced his retirement from Test and One-Day cricket in early 2008.


title: Adam Gilchrist

text: In March 2008, Gilchrist joined the Nine Network. Gilchrist has appeared as one of a panel of revolving co-hosts for the revived "Wide World of Sports Weekend Edition". He made his debut on the program in March 2008, and commentates on Nine's cricket coverage during the Australian summer. In 2013 Gilchrist joined Ricky Ponting and various other names in cricket to commentate for Channel Ten in the third series of the Big Bash League.


title: Adam Gilchrist

text: As Amway Australia Ambassador, Gilchrist has played a role in many of their charity events. In August 2010, he presented the Freedom Wheels program, an initiative to provide modified bikes to kids with disabilities, a cheque for $20,000.


title: Adam Gilchrist

text: Gilchrist was the chair of the National Australia Day Council from 2008 to 2014. In 2008, Gilchrist supported debate on whether Australia Day should be moved to a new date because the current date marks British settlement of New South Wales and is offensive to many Aboriginal Australians.


title: Adam Gilchrist

text: Gilchrist has had a number of company directorships outside of cricket. His appointment to the board of ASX listed sandalwood company TFS Corporation, committee member of Commonwealth Business Forum in Perth and director of Travelex. The appointment to TFS Corporation was not without controversy when as a board member of TFS he was named as a plaintiff suing his own TFS shareholders for defamation


title: Alexander Kolchak

text: Alexander Vasilyevich Kolchak (; – 7 February 1920) was an Imperial Russian admiral, military leader and polar explorer who served in the Imperial Russian Navy and fought in the Russo-Japanese War of 1904–1905 and the First World War. During the Russian Civil War of 1917–1922 he established an anti-communist government in Siberia — later the Provisional All-Russian Government — and became recognized as the "Supreme Leader and Commander-in-Chief of All Russian Land and Sea Forces" by the other leaders of the White movement from 1918 to 1920. His government was based in Omsk, in southwestern Siberia.


title: Alexander Kolchak

text: For nearly two years, Kolchak served as Russia's internationally recognized head of state. However, his efforts to unite the White Movement failed; Kolchak refused to consider autonomy for ethnic minorities and refused to cooperate with non-Bolshevik leftists, looking for foreign support instead. This served only to boost the Bolsheviks' morale, as it allowed them to label Kolchak as a "Western Puppet". As his White forces fell apart, he was betrayed and detained by the chief of the Allied military mission in Siberia, Maurice Janin and the Czechoslovak Legion, (December 1919) who handed him over to local Socialist-Revolutionaries in January 1920; soon afterwards the Bolsheviks executed him in February 1920, in Irkutsk.


title: Alexander Kolchak

text: Kolchak was born in Saint Petersburg in 1874 to a family of Romanian origins. Both of his parents were from Odessa. His father was a retired major-general of the Marine Artillery and a veteran of the 1854 siege of Sevastopol, who after retirement worked as an engineer in ordnance works near St. Petersburg. Kolchak was educated for a naval career, graduating from the Naval Cadet Corps in 1894 and joining the 7th Naval Battalion. He was soon transferred to the Russian Far East, serving in Vladivostok from 1895 to 1899. He then returned to western Russia and was based at Kronstadt, joining the Russian Polar expedition of Eduard Toll on the ship "Zarya" in 1900 as a hydrologist.


title: Alexander Kolchak

text: After considerable hardship, Kolchak returned in December 1902; Eduard Toll, along with three other explorers continued further north and were lost. Kolchak took part in two Arctic expeditions to look for the lost explorers (who were not found) and for a while was nicknamed "Kolchak-Poliarnyi" ("Kolchak the Polar"). For his explorations Kolchak received the Constantine Medal, the highest award of the Imperial Russian Geographical Society.


title: Alexander Kolchak

text: In December 1903, Kolchak was en route to St. Petersburg to marry his fiancée, Sophia Omirova, when, not far from Irkutsk, he received notice of the start of war with the Empire of Japan and hastily summoned his bride and her father to Siberia by telegram for a wedding, before heading directly to Port Arthur. In the early stages of the Russo-Japanese War, he served as a watch officer on the cruiser , and later commanded the destroyer "Serdity". He made several night sorties to lay naval mines, one of which succeeded in sinking the Japanese cruiser . He was decorated with the Order of St. Anna 4th class for the exploit. As the blockade of the port tightened and the Siege of Port Arthur intensified, he was given command of a coastal artillery battery. He was wounded in the final battle for Port Arthur and taken as a prisoner of war to Nagasaki, where he spent four months. His poor health (rheumatism, a consequence of his polar expeditions) led to his repatriation before the end of the war. Kolchak was awarded the Golden Sword of St. George with the inscription "For Bravery" on his return to Russia.


title: Alexander Kolchak

text: Returning to Saint Petersburg in April 1905, Kolchak was promoted to lieutenant commander and took part in rebuilding of the Imperial Russian Navy, which had been almost completely destroyed during the war. He served on the Naval General Staff from 1906, helping draft a shipbuilding program, a training program, and developing a new protection plan for St. Petersburg and the Gulf of Finland.


title: Alexander Kolchak

text: Kolchak took part in designing special icebreakers "Taimyr" and "Vaigach", launched in 1909 and spring of 1910. Based in Vladivostok, these vessels were sent on a cartographic expedition to the Bering Strait and Cape Dezhnev. Kolchak commanded the "Vaigach" during this expedition and later worked at the Academy of Sciences with the materials collected by him during expeditions. His study, "Ice of the Kara and Siberian Seas", was printed in the Proceedings of the Russian Imperial Academy of Sciences and is considered the most important work on this subject. Extracts from it were published under the title "The Arctic Pack and the Polynya" in the volume issued in 1929 by the American Geographical Society, "Problems of Polar Research".


title: Alexander Kolchak

text: In 1910 he returned to the Naval General Staff, and in 1912 he was assigned to the Russian Baltic Fleet.


title: Alexander Kolchak

text: The onset of the First World War found him on the flagship "Pogranichnik", where Kolchak oversaw laying of extensive coastal defensive minefields and commanded the naval forces in the Gulf of Riga. Commanding Admiral Essen was not satisfied to remain on the defensive and ordered Kolchak to prepare a scheme for attacking the approaches of the German naval bases. During the autumn and winter of 1914–1915, Russian destroyers and cruisers started a series of dangerous night operations, laying mines at the approaches to Kiel and Danzig. Kolchak, feeling that the man responsible for planning operations should also take part in their execution, was always on board those ships which carried out the operations and at times took direct command of the destroyer flotillas.


title: Alexander Kolchak

text: He was promoted to vice-admiral in August 1916, the youngest man at that rank, and was made commander of the Black Sea Fleet, replacing Admiral Eberhardt. Kolchak's primary mission was to support General Yudenich in his operations against the Ottoman Empire. He also was tasked with countering the U-boat threat and planning the invasion of the Bosphorus (never carried out). Kolchak's fleet was successful at sinking Turkish colliers. Because there was no railroad linking the coal mines of eastern Turkey with Constantinople, the Russian fleet's attacks on these Turkish coal ships caused the Ottoman government much hardship. In 1916, in a combined Army-Navy assault, the Russian Black Sea fleet aided the Russian army's capture of the Ottoman city of Trebizond (modern Trabzon).


title: Alexander Kolchak

text: One notable disaster took place under Kolchak's watch: the dreadnought "Imperatritsa Mariya" exploded in port at Sevastopol on 7 October 1916. A careful investigation failed to determine whether the cause of the disaster was accident or sabotage.


title: Alexander Kolchak

text: The Black Sea fleet descended into political chaos after the onset of the 1917 February Revolution. Kolchak was relieved of command of the fleet in June and traveled to Petrograd (St. Petersburg). On his arrival at Petrograd, Kolchak was invited to a meeting of the Provisional Government. There he presented his view on the condition of the Russian armed forces and their complete demoralisation. He stated that the only way to save the country was to re-establish strict discipline and restore capital punishment in the army and navy.


title: Alexander Kolchak

text: During this time many organisations and newspapers of a conservative inclination spoke of him as a future dictator. A number of new and secret organisations had sprung up in Petrograd with the goal of suppressing the Bolshevist movement and removal of the extremist members of the government. Some of these organisations asked Kolchak to accept the leadership.


title: Alexander Kolchak

text: When news of these plots found their way to then Naval Minister of the Provisional Government, Alexander Kerensky, he ordered Kolchak to leave immediately for America. Admiral James H. Glennon, a member of American mission headed by Senator Elihu Root, invited Kolchak to the United States to brief the American Navy on the strategic situation in the Bosphorus. On 19 August 1917 Kolchak with several officers left Petrograd for Britain and the United States as a quasi-official military observer. When passing through London he was greeted cordially by the First Sea Lord, Admiral Sir John Jellicoe, who offered him transport on board a British cruiser on his way to Halifax in Canada. The journey to America proved unnecessary, as by the time Kolchak arrived, the US had given up the idea of any independent action in the Dardanelles. Kolchak visited the American Fleet and its ports and decided to return to Russia via Japan.


title: Alexander Kolchak

text: The Bolshevik revolution in November 1917 found Kolchak in Japan. In December, he visited the British embassy in Tokyo and offered his services "unconditionally and in whatever capacity" to the British. Two years later, when interrogated by the Bolsheviks, he explained that as a supporter of the Provisional Government, he considered himself honour bound to continue to fight the war with Germany, and, understanding that there was no suitable role in the British Navy for a Russian admiral, he would be prepared to fight as a private in the British army. His offer was referred up to the Foreign Secretary, Arthur Balfour, and accepted on 29 December. He was instructed to join the British military mission in Baghdad, but when he reached Singapore, was ordered to turn back and go via Shanghai and Beijing to Harbin, to take command of Russian troops guarding the Russian-owned Chinese Eastern Railway in Manchuria, which the British government had decided could be a base for overthrowing the Bolshevik government and getting Russia back into the war with Germany. Arriving in Omsk, Siberia, en route to enlisting with the Volunteer Army, he agreed to become a minister in the (White) Siberian Regional Government. Joining a 14-man cabinet, he was a prestige figure; the government hoped to play on the respect he had with the Allies, especially the head of the British military mission, General Alfred Knox. Knox wrote that Kolchak had "more grit, pluck and honest patriotism than any Russian in Siberia".


title: Alexander Kolchak

text: According to historian Richard Pipes, Kolchak was a man with poor social skills, being moody, melancholic, taciturn, and very uncomfortable in dealing with people. Arriving at a dinner, Colonel John Ward described him as "a small, vagrant, lonely soul without a friend enter unbidden to a feast". One who knew him wrote:


title: Alexander Kolchak

text: The character and soul of the Admiral are so transparent that one needs no more than one week of contact to know all there is to know about him. He is a big, sick child, a pure idealist, a convinced slave of duty and service to an idea and to Russia. An indubitable neurotic who quickly flares up, exceedingly impetuous and uncontrolled in expressions of displeasure and anger; in this respect he has assimilated the highly unattractive traditions of the naval service, which permit in high naval ranks behavior that in our army has long since passed into the realm of legend. He is utterly absorbed by the idea of serving Russia, of saving her from Red oppression, and restoring her to full power and to the inviolability of her territory. For the sake of this he can be persuaded and moved to do anything whatever. He has no personal interests, no "amour propre": in this respect he is crystal pure. He passionately despises all lawlessness and arbitrariness, but because he is so uncontrolled and impulsive, he himself often unintentionally transgresses against the law, and this mainly when seeking to uphold the same law, and always under the influence of some outsider. He does not know life in its severe, practical reality, and lives in a world of mirages and borrowed ideas. He has no plans, no system, no will: in this respect he is soft wax from which advisers and intimates can fashion whatever they want, exploiting the fact that it is enough to disguise something as necessary for the welfare of Russia and the good of the cause to be certain of his approval.


title: Alexander Kolchak

text: He is kind and at the same time severe, responsive and at the same time embarrassed to show human feelings, concealing his gentleness behind make-believe severity. He is impatient and stubborn, loses his temper, threatens and then calms down, making concessions, spreads his hands in a gesture of helplessness. He is bursting to be with the people, with the troops, but when he faces them, has no idea of what to say.


title: Alexander Kolchak

text: Politically naive and an inept administrator, Kolchak described himself as a "military technician" who knew nothing of politics, described power as a "cross", and in a letter to his wife wrote about the "terrifying burden of Supreme Power" and admitted that as "a fighting man he was reluctant to face the problems of statecraft". The American historian Richard Pipes wrote that Kolchak's only strengths were his courage, patriotism, integrity, and a strong sense of honor, writing that he was "...in many ways, along with Wrangel, the most honorable White commander in the Civil War", but his weaknesses, such as his tendency to suffer from manic depression and inability to "understand people or communicate with them", made him into "an execrable administrator in whose name were committed unpardonable acts of corruption and brutality that he personally found utterly repugnant."


title: Alexander Kolchak

text: In November 1918, the unpopular regional government was overthrown in a British sponsored "coup d'etat". Kolchak had returned to Omsk on 16 November from an inspection tour. He was approached and refused to take power. The Socialist-Revolutionary (SR) directory leader and members were arrested on 18 November by a troop of Cossacks under ataman Krasilnikov. The remaining cabinet members met and voted for Kolchak to become the head of government with emergency powers. He was named Supreme Ruler ("Verkhovnyi Pravitel"), and he promoted himself to full admiral. The arrested SR politicians were expelled from Siberia and ended up in Europe.


title: Alexander Kolchak

text: The Left SR leaders in Russia denounced Kolchak and called for his assassination. Their activities resulted in a small revolt in Omsk on 22 December 1918, which was quickly put down by Cossacks and the Czechoslovak Legion, who summarily executed almost 500 rebels. Subsequently, the SRs opened negotiations with the Bolsheviks and in January 1919 the SR People's Army joined up with the Red Army.


title: Alexander Kolchak

text: Kolchak pursued a policy of persecuting revolutionaries as well as Socialists of several factions. His government issued a decree on 3 December 1918 stating, "In order to preserve the system and rule of the Supreme Ruler, articles of the criminal code of Imperial Russia were revised, Articles 99 and 100 of which established capital punishment for assassination attempts on the Supreme Ruler and for attempting to overthrow his government." Insults written, printed, and oral, are punishable by imprisonment under Article 103. Bureaucratic sabotage under Article 329 was punishable by hard labour from 15 to 20 years.


title: Alexander Kolchak

text: Although the news of Kolchak's ascension to power spread very slowly behind Bolshevik lines, it caused considerable excitement among anti-communist Russians living there. Ivan Bunin wrote in his diary, "4/17 June 1919. The Entente has named Kolchak the Supreme Ruler of Russia. "Izvestia" wrote an obscene article saying: 'Tell us, you reptile, how much did they pay you for that?' The devil with them. I crossed myself with tears of joy."


title: Alexander Kolchak

text: On 11 April 1919, the Kolchak government adopted Regulation no. 428, "About dangers to public order due to ties with the Bolshevik Revolt". The legislation was published in the Omsk newspaper "Omsk Gazette" (no. 188 of 19 July 1919). It provided a term of 5 years imprisonment for "individuals considered a threat to the public order because of their ties in any way with the Bolshevik revolt." In the case of unauthorized return from exile, there could be hard labour from 4 to 8 years. Articles 99–101 allowed the death penalty, forced labour and imprisonment, repression by military courts, and imposed no investigation commissions.


title: Alexander Kolchak

text: Kolchak acknowledged all of Russia's debts, returned nationalized factories and plants to their owners, granted concessions to foreign investors, dispersed trade unions, persecuted Marxists, and disbanded the soviets. Kolchak's agrarian policy was directed toward restoring private land ownership. To this end former Tsarist laws concerning property were restored.


title: Alexander Kolchak

text: On 26 May 1919, the Supreme War Council in Paris offered to provide Kolchak with unlimited supplies of food, weapons, munitions and other supplies (but not diplomatic recognition) provided that he was willing to meet the following conditions:


title: Alexander Kolchak

text: Pipes wrote that though the Allies wanted a Constituent Assembly to decide the future of Russia, they had decided in advance in their conditions that, for instance, there would be no restoration of the monarchy as well as many other matters that properly should have been decided by the Constituent Assembly. Because Kolchak was entirely dependent upon supplies from Britain—the British had shipped him in the period October 1918-October 1919 about 600,000 rifles, 6,831 machine guns, and about 200,000 uniforms—he had to accept nearly all of the conditions. In a telegram to Paris sent on 4 June 1919, Kolchak accepted every condition except for the independence of Finland, which he accepted only "de facto", not "de jure", saying he wanted the Constituent Assembly to grant Finland its independence. As the Allies were especially opposed to a return of the House of Romanov, Kolchak emphatically declared "that there cannot be a return to the regime which existed in Russia before February 1917." The British War Secretary Winston Churchill pressed very strongly in the cabinet for British recognition of Kolchak's government, but the Prime Minister David Lloyd George would only do so if the United States likewise recognized Kolchak. The American president Woodrow Wilson was strongly hostile towards Kolchak, openly doubted his word, and was against diplomatic recognition. Wilson's main adviser on Russia was the former head of the Provisional Government, Alexander Kerensky, who told Wilson that Kolchak was a "reactionary" who would "inaugurate a regime hardly less sanguinary and repressive than that of the Bolsheviks." Though American forces in Siberia co-operated with Kolchak, it was clear he was not the man favored by the United States as the next leader of Russia. American forces had been sent to Siberia less to help the Whites than to prevent the Japanese, who had occupied the Russian Far East, from annexing it as Tokyo was openly considering.


title: Alexander Kolchak

text: The Great Soviet Encyclopedia alleges that more than 25,000 people were shot or tortured to death in the Yekaterinburg Governorate alone. In March 1919 Kolchak himself demanded of one of his generals that he "follow the example of the Japanese who, in the Amur region, had exterminated the local population." "Sovietskaya Rossiya", an official organ of the Soviet Bureau established by Ludwig Martens, quoted a Menshevik organ, "Vsegda Vperyod", alleging that Kolchak's men used mass floggings and razed entire villages to the ground with artillery fire. 4,000 peasants allegedly became victims of field courts and punitive expeditions and that all dwellings of rebels were burned down.


title: Alexander Kolchak

text: In an excerpt from the order of the government of Yenisei county in Irkutsk province, General. S. Rozanov said:


title: Alexander Kolchak

text: There was prominent underground resistance in the regions controlled by Kolchak's government. These partisans were especially strong in the provinces of Altai and Yeniseysk. In summer 1919 partisans of the Altai Region united to form the Western Siberian Peasants' Red Army (25,000 men). The Taseev Soviet Partisan Republic was founded south-east of Yeniseysk in early 1919. By the fall of 1919, Kolchak's rear was completely disintegrating. About 100,000 Siberian partisans seized vast regions from Kolchak's regime even before the approach of the Red Army. In February 1920, some 20,000 partisans took control of the Amur region.


title: Alexander Kolchak

text: Initially the White forces under his command had some success. Kolchak was unfamiliar with combat on land and gave the majority of the strategic planning to D. A. Lebedev, Paul J. Bubnar, and his staff. The northern army under the Russian Anatoly Pepelyayev and the Czech Rudolf Gajda seized Perm in late December 1918 and after a pause other forces spread out from this strategic base. The plan was for three main advances – Gajda to take Archangel, Khanzhin to capture Ufa and the Cossacks under Alexander Dutov to capture Samara and Saratov.


title: Alexander Kolchak

text: The White forces took Ufa in March 1919 and pushed on from there to take Kazan and approach Samara on the Volga River. Anti-Communist risings in Simbirsk, Kazan, Viatka, and Samara assisted their endeavours. The newly formed Red Army proved unwilling to fight and retreated instead, allowing the Whites to advance to a line stretching from Glazov through Orenburg to Uralsk. Kolchak's territories covered over 300,000 km and held around 7 million people. In April, the alarmed Bolshevik Central Executive Committee made defeating Kolchak its top priority. But as the spring thaw arrived Kolchak's position degenerated – his armies had outrun their supply lines, they were exhausted, and the Red Army poured newly raised troops into the area.


title: Alexander Kolchak

text: Kolchak had also aroused dislike of potential allies, including the Czechoslovak Legion and the Polish 5th Rifle Division. They withdrew from the conflict in October 1918 but remained a presence; their foreign adviser General Maurice Janin regarded Kolchak as an instrument of the British and himself was pro-SR. Kolchak could not count on Japanese aid either; the Japanese feared he would interfere with their occupation of Far Eastern Russia and refused him assistance, creating a buffer state to the east of Lake Baikal under Cossack control. The 7,000 or so American troops in Siberia were strictly neutral regarding "internal Russian affairs" and served only to maintain the operation of the Trans-Siberian railroad in the Far East. The American commander, General William S. Graves, personally disliked the Kolchak government, which he saw as monarchist and autocratic, a view that was shared by the American President, Woodrow Wilson.


title: Alexander Kolchak

text: From 1919, when the Bolshevik forces managed to reorganise and turn the attack against Kolchak, he quickly lost ground. The Red counter-attack began in late April at the centre of the White line, aiming for Ufa. The fighting was fierce as, unlike earlier, both sides fought hard. Ufa was taken by the Red Army on 9 June and later that month the Red forces under Mikhail Tukhachevsky broke through the Urals. Freed from the geographical constraints of the mountains, the Reds made rapid progress, capturing Chelyabinsk on 25 July and forcing the White forces to the north and south to fall back to avoid being isolated. The White forces re-established a line along the Tobol and the Ishim rivers to temporarily halt the Reds. They held that line until October, but the constant loss of men killed or wounded was beyond the White rate of replacement. Reinforced, the Reds broke through on the Tobol in mid-October and by November the White forces were falling back towards Omsk in a disorganised mass. At this point the Reds became sufficiently confident to start redeploying some of their forces southwards to face Anton Denikin.


title: Alexander Kolchak

text: Kolchak also came under threat from other quarters: local opponents began to agitate and international support began to wane, with even the British turning more towards Denikin. Gajda, dismissed from command of the northern army, staged an abortive coup in mid-November. Omsk was evacuated on 14 November, and the Red Army took the city without any serious resistance, capturing large amounts of ammunition, almost 50,000 soldiers, and ten generals. As there was a continued flood of refugees eastwards, typhus too became a serious problem.


title: Alexander Kolchak

text: Kolchak had left Omsk on the 13th for Irkutsk along the Trans-Siberian Railroad. Travelling a section of track controlled by the Czechoslovaks, he was sidetracked and stopped; by December his train had only reached Nizhneudinsk. In late December Irkutsk fell under control of a leftist group (including SRs and Mensheviks) and formed the Political Centre. One of their first actions was to dismiss Kolchak. When he heard of this on 4 January 1920, he announced his resignation, giving his office to Denikin and passing control of his remaining forces around Irkutsk to an ataman, G. M. Semyonov. The transfer of power to Semyonov proved a particularly ill-considered move.


title: Alexander Kolchak

text: Kolchak was then promised safe passage by the Czechoslovaks to the British military mission in Irkutsk. Instead, he was handed over to the Left SR authorities in Irkutsk on 14 January. On 20 January the government in Irkutsk surrendered power to a Bolshevik military committee. The White Army under the command of General Vladimir Kappel advanced toward Irkutsk while Kolchak was interrogated by a commission of five men representing the Revolutionary Committee (REVKOM) during nine days between 21 January and 6 February. Despite the arrival of a contrary order from Moscow, Admiral Kolchak was sentenced to death along with his Prime Minister, Viktor Pepelyayev.


title: Alexander Kolchak

text: Both prisoners were brought before a firing squad in the late night of 6 February 1920. According to eyewitnesses, Kolchak was entirely calm and unafraid, "like an Englishman." The Admiral asked the commander of the firing squad, "Would you be so good as to get a message sent to my wife in Paris to say that I bless my son?" The commander responded, "I'll see what can be done, if I don't forget about it."


title: Alexander Kolchak

text: A priest of the Russian Orthodox Church then gave the last rites to both men. The squad fired and both men fell. The bodies were kicked and prodded down an escarpment and dumped under the ice of the frozen Angara River. Their bodies were never recovered. When the White Army learned about the executions, its remaining leadership decided to withdraw farther east. The Great Siberian Ice March followed. The Red Army did not enter Irkutsk until 7 March, and only then was the news of Kolchak's death officially released.


title: Alexander Kolchak

text: Admiral Kolchak's government was not successful from the time of his taking the position of "Supreme Ruler" until his death. As a military commander he was unable to make successful strategic plans or to coordinate with other White Army generals such as Yudenich or Denikin.


title: Alexander Kolchak

text: Kolchak also failed to convince potentially friendly Finland to join with him against the Bolsheviks. He was unable to win diplomatic recognition from any nation in the world, even the United Kingdom (though the British did support him to some degree). In addition he alienated the Czechoslovak Legion, which for a time was a powerful organised military force in the region and very strongly anti-Bolshevik. As was mentioned above, the American commander, General Graves, disliked Kolchak and refused to lend him any military aid at all.


title: Alexander Kolchak

text: After decades of being vilified by the Soviet government, Kolchak is now a controversial historic figure in post-Soviet Russia. The For Faith and Fatherland movement has attempted to rehabilitate his reputation. However, two rehabilitation requests have been denied, by a regional military court in 1999 and by the Supreme Court of the Russian Federation in 2001. In 2004, the Constitutional Court of Russia returned the Kolchak case to the military court for another hearing.


title: Alexander Kolchak

text: Monuments dedicated to Kolchak were built in Saint Petersburg in 2002 and in Irkutsk in 2004, despite objections from some former Communist and left-wing politicians, as well as former Soviet army veterans. His memorial in St. Petersburg is a frequent target of vandalism. There is also a Kolchak Island. The modern Russian Navy considered naming the third ship of the new s, "Admiral Kolchak" to commemorate the Admiral but declined to do so in the end.


title: Alexander Kolchak

text: Kolchak was a prominent expert on naval mines and a member of the Russian Geographical Society. Among his awards are the Saint George Gold Sword for Bravery, given for his actions in the battle of Port Arthur and the Great Gold Constantine Medal from the Russian Geographic Society.


title: Alexander Kolchak

text: A Kolchak biographical film, titled "Admiral" (Адмиралъ), was released in Russia on 9 October 2008 after a gala preview to an audience of senior Russian Navy admirals. The film portrays the Admiral (Konstantin Khabensky) as a tragic hero with a deep love for his country. Elizaveta Boyarskaya appears as his common law wife, the poet, Anna Timireva. Director Andrei Kravchuk described the film as follows,


title: Alexander Kolchak

text: It's about a man who tries to create history, to take an active part in history, as he gets caught in the turmoil. However, he keeps on struggling, he preserves his honor and his dignity, and he continues to love.


title: Alexander Kolchak

text: In the shared alternate history of Ill Bethisad (1997 and after), The Kolchak-led White Army winning the Russian Civil War is a major point of divergence from actual history in the timeline of the fictional alternate universe.


title: Gel electrophoresis

text: Gel electrophoresis is a method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.


title: Gel electrophoresis

text: Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by the charge in agarose because the pores of the gel are too small to sieve proteins. Gel electrophoresis can also be used for the separation of nanoparticles.


title: Gel electrophoresis

text: Gel electrophoresis uses a gel as an anticonvective medium or sieving medium during electrophoresis, the movement of a charged particle in an electrical current. Gels suppress the thermal convection caused by the application of the electric field, and can also act as a sieving medium, slowing the passage of molecules; gels can also simply serve to maintain the finished separation so that a post electrophoresis stain can be applied. DNA gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via polymerase chain reaction (PCR), but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.


title: Gel electrophoresis

text: Electrophoresis is a process that enables the sorting of molecules based on size. Using an electric field, molecules (such as DNA) can be made to move through a gel made of agarose or polyacrylamide. The electric field consists of a negative charge at one end which pushes the molecules through the gel, and a positive charge at the other end that pulls the molecules through the gel. The molecules being sorted are dispensed into a well in the gel material. The gel is placed in an electrophoresis chamber, which is then connected to a power source. When the electric field is applied, the larger molecules move more slowly through the gel while the smaller molecules move faster. The different sized molecules form distinct bands on the gel.


title: Gel electrophoresis

text: The term "gel" in this instance refers to the matrix used to contain, then separate the target molecules. In most cases, the gel is a crosslinked polymer whose composition and porosity are chosen based on the specific weight and composition of the target to be analyzed. When separating proteins or small nucleic acids (DNA, RNA, or oligonucleotides) the gel is usually composed of different concentrations of acrylamide and a cross-linker, producing different sized mesh networks of polyacrylamide. When separating larger nucleic acids (greater than a few hundred bases), the preferred matrix is purified agarose. In both cases, the gel forms a solid, yet porous matrix. Acrylamide, in contrast to polyacrylamide, is a neurotoxin and must be handled using appropriate safety precautions to avoid poisoning. Agarose is composed of long unbranched chains of uncharged carbohydrates without cross-links resulting in a gel with large pores allowing for the separation of macromolecules and macromolecular complexes.


title: Gel electrophoresis

text: Electrophoresis refers to the electromotive force (EMF) that is used to move the molecules through the gel matrix. By placing the molecules in wells in the gel and applying an electric field, the molecules will move through the matrix at different rates, determined largely by their mass when the charge-to-mass ratio (Z) of all species is uniform. However, when charges are not all uniform the electrical field generated by the electrophoresis procedure will cause the molecules to migrate differentially according to charge. Species that are net positively charged will migrate towards the cathode which is negatively charged (because this is an electrolytic rather than galvanic cell), whereas species that are net negatively charged will migrate towards the positively charged anode. Mass remains a factor in the speed with which these non-uniformly charged molecules migrate through the matrix toward their respective electrodes.


title: Gel electrophoresis

text: If several samples have been loaded into adjacent wells in the gel, they will run parallel in individual lanes. Depending on the number of different molecules, each lane shows the separation of the components from the original mixture as one or more distinct bands, one band per component. Incomplete separation of the components can lead to overlapping bands, or indistinguishable smears representing multiple unresolved components. Bands in different lanes that end up at the same distance from the top contain molecules that passed through the gel at the same speed, which usually means they are approximately the same size. There are molecular weight size markers available that contain a mixture of molecules of known sizes. If such a marker was run on one lane in the gel parallel to the unknown samples, the bands observed can be compared to those of the unknown to determine their size. The distance a band travels is approximately inversely proportional to the logarithm of the size of the molecule (alternatively, this can be stated as the distance traveled is inversely proportional to the log of samples's molecular weight).


title: Gel electrophoresis

text: There are limits to electrophoretic techniques. Since passing a current through a gel causes heating, gels may melt during electrophoresis. Electrophoresis is performed in buffer solutions to reduce pH changes due to the electric field, which is important because the charge of DNA and RNA depends on pH, but running for too long can exhaust the buffering capacity of the solution. There are also limitations in determining the molecular weight by SDS-PAGE, especially when trying to find the MW of an unknown protein. Certain biological variables are difficult or impossible to minimize and can affect electrophoretic migration. Such factors include protein structure, post-translational modifications, and amino acid composition. For example, tropomyosin is an acidic protein that migrates abnormally on SDS-PAGE gels. This is because the acidic residues are repelled by the negatively charged SDS, leading to an inaccurate mass-to-charge ratio and migration. Further, different preparations of genetic material may not migrate consistently with each other, for morphological or other reasons.


title: Gel electrophoresis

text: The types of gel most typically used are agarose and polyacrylamide gels. Each type of gel is well-suited to different types and sizes of the analyte. Polyacrylamide gels are usually used for proteins and have very high resolving power for small fragments of DNA (5-500 bp). Agarose gels, on the other hand, have lower resolving power for DNA but have a greater range of separation, and are therefore used for DNA fragments of usually 50–20,000 bp in size, but the resolution of over 6 Mb is possible with pulsed field gel electrophoresis (PFGE). Polyacrylamide gels are run in a vertical configuration while agarose gels are typically run horizontally in a submarine mode. They also differ in their casting methodology, as agarose sets thermally, while polyacrylamide forms in a chemical polymerization reaction.


title: Gel electrophoresis

text: Agarose gels are easily cast and handled compared to other matrices because the gel setting is a physical rather than chemical change. Samples are also easily recovered. After the experiment is finished, the resulting gel can be stored in a plastic bag in a refrigerator.


title: Gel electrophoresis

text: Agarose gels do not have a uniform pore size, but are optimal for electrophoresis of proteins that are larger than 200 kDa. Agarose gel electrophoresis can also be used for the separation of DNA fragments ranging from 50 base pair to several megabases (millions of bases), the largest of which require specialized apparatus. The distance between DNA bands of different lengths is influenced by the percent agarose in the gel, with higher percentages requiring longer run times, sometimes days. Instead high percentage agarose gels should be run with a pulsed field electrophoresis (PFE), or field inversion electrophoresis.


title: Gel electrophoresis

text: "Most agarose gels are made with between 0.7% (good separation or resolution of large 5–10kb DNA fragments) and 2% (good resolution for small 0.2–1kb fragments) agarose dissolved in electrophoresis buffer. Up to 3% can be used for separating very tiny fragments but a vertical polyacrylamide gel is more appropriate in this case. Low percentage gels are very weak and may break when you try to lift them. High percentage gels are often brittle and do not set evenly. 1% gels are common for many applications."


title: Gel electrophoresis

text: Polyacrylamide gel electrophoresis (PAGE) is used for separating proteins ranging in size from 5 to 2,000 kDa due to the uniform pore size provided by the polyacrylamide gel. Pore size is controlled by modulating the concentrations of acrylamide and bis-acrylamide powder used in creating a gel. Care must be used when creating this type of gel, as acrylamide is a potent neurotoxin in its liquid and powdered forms.


title: Gel electrophoresis

text: Traditional DNA sequencing techniques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in length so the sequence could be read. Most modern DNA separation methods now use agarose gels, except for particularly small DNA fragments. It is currently most often used in the field of immunology and protein analysis, often used to separate different proteins or isoforms of the same protein into separate bands. These can be transferred onto a nitrocellulose or PVDF membrane to be probed with antibodies and corresponding markers, such as in a western blot.


title: Gel electrophoresis

text: Typically resolving gels are made in 6%, 8%, 10%, 12% or 15%. Stacking gel (5%) is poured on top of the resolving gel and a gel comb (which forms the wells and defines the lanes where proteins, sample buffer, and ladders will be placed) is inserted. The percentage chosen depends on the size of the protein that one wishes to identify or probe in the sample. The smaller the known weight, the higher the percentage that should be used. Changes in the buffer system of the gel can help to further resolve proteins of very small sizes.


title: Gel electrophoresis

text: Partially hydrolysed potato starch makes for another non-toxic medium for protein electrophoresis. The gels are slightly more opaque than acrylamide or agarose. Non-denatured proteins can be separated according to charge and size. They are visualised using Napthal Black or Amido Black staining. Typical starch gel concentrations are 5% to 10%.


title: Gel electrophoresis

text: Denaturing gels are run under conditions that disrupt the natural structure of the analyte, causing it to unfold into a linear chain. Thus, the mobility of each macromolecule depends only on its linear length and its mass-to-charge ratio. Thus, the secondary, tertiary, and quaternary levels of biomolecular structure are disrupted, leaving only the primary structure to be analyzed.


title: Gel electrophoresis

text: Nucleic acids are often denatured by including urea in the buffer, while proteins are denatured using sodium dodecyl sulfate, usually as part of the SDS-PAGE process. For full denaturation of proteins, it is also necessary to reduce the covalent disulfide bonds that stabilize their tertiary and quaternary structure, a method called reducing PAGE. Reducing conditions are usually maintained by the addition of beta-mercaptoethanol or dithiothreitol. For a general analysis of protein samples, reducing PAGE is the most common form of protein electrophoresis.


title: Gel electrophoresis

text: Denaturing conditions are necessary for proper estimation of molecular weight of RNA. RNA is able to form more intramolecular interactions than DNA which may result in change of its electrophoretic mobility. Urea, DMSO and glyoxal are the most often used denaturing agents to disrupt RNA structure. Originally, highly toxic methylmercury hydroxide was often used in denaturing RNA electrophoresis, but it may be method of choice for some samples.


title: Gel electrophoresis

text: Denaturing gel electrophoresis is used in the DNA and RNA banding pattern-based methods temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE).


title: Gel electrophoresis

text: Native gels are run in non-denaturing conditions so that the analyte's natural structure is maintained. This allows the physical size of the folded or assembled complex to affect the mobility, allowing for analysis of all four levels of the biomolecular structure. For biological samples, detergents are used only to the extent that they are necessary to lyse lipid membranes in the cell. Complexes remain—for the most part—associated and folded as they would be in the cell. One downside, however, is that complexes may not separate cleanly or predictably, as it is difficult to predict how the molecule's shape and size will affect its mobility. Addressing and solving this problem is a major aim of quantitative native PAGE.


title: Gel electrophoresis

text: Unlike denaturing methods, native gel electrophoresis does not use a charged denaturing agent. The molecules being separated (usually proteins or nucleic acids) therefore differ not only in molecular mass and intrinsic charge, but also the cross-sectional area, and thus experience different electrophoretic forces dependent on the shape of the overall structure. For proteins, since they remain in the native state they may be visualized not only by general protein staining reagents but also by specific enzyme-linked staining.


title: Gel electrophoresis

text: A specific experiment example of an application of native gel electrophoresis is to check for enzymatic activity to verify the presence of the enzyme in the sample during protein purification. For example, for the protein alkaline phosphatase, the staining solution is a mixture of 4-chloro-2-2methylbenzenediazonium salt with 3-phospho-2-naphthoic acid-2'-4'-dimethyl aniline in Tris buffer. This stain is commercially sold as a kit for staining gels. If the protein is present, the mechanism of the reaction takes place in the following order: it starts with the de-phosphorylation of 3-phospho-2-naphthoic acid-2'-4'-dimethyl aniline by alkaline phosphatase (water is needed for the reaction). The phosphate group is released and replaced by an alcohol group from water. The electrophile 4- chloro-2-2 methylbenzenediazonium (Fast Red TR Diazonium salt) displaces the alcohol group forming the final product Red Azo dye. As its name implies, this is the final visible-red product of the reaction. In undergraduate academic experimentation of protein purification, the gel is usually run next to commercial purified samples to visualize the results and conclude whether or not purification was successful.


title: Gel electrophoresis

text: Native gel electrophoresis is typically used in proteomics and metallomics. However, native PAGE is also used to scan genes (DNA) for unknown mutations as in Single-strand conformation polymorphism.


title: Gel electrophoresis

text: Buffers in gel electrophoresis are used to provide ions that carry a current and to maintain the pH at a relatively constant value.


title: Gel electrophoresis

text: These buffers have plenty of ions in them, which is necessary for the passage of electricity through them. Something like distilled water or benzene contains few ions, which is not ideal for the use in electrophoresis. There are a number of buffers used for electrophoresis. The most common being, for nucleic acids Tris/Acetate/EDTA (TAE), Tris/Borate/EDTA (TBE). Many other buffers have been proposed, e.g. lithium borate, which is rarely used, based on Pubmed citations (LB), isoelectric histidine, pK matched goods buffers, etc.; in most cases the purported rationale is lower current (less heat) matched ion mobilities, which leads to longer buffer life. Borate is problematic; Borate can polymerize, or interact with cis diols such as those found in RNA. TAE has the lowest buffering capacity but provides the best resolution for larger DNA. This means a lower voltage and more time, but a better product. LB is relatively new and is ineffective in resolving fragments larger than 5 kbp; However, with its low conductivity, a much higher voltage could be used (up to 35 V/cm), which means a shorter analysis time for routine electrophoresis. As low as one base pair size difference could be resolved in 3% agarose gel with an extremely low conductivity medium (1 mM Lithium borate).


title: Gel electrophoresis

text: Most SDS-PAGE protein separations are performed using a "discontinuous" (or DISC) buffer system that significantly enhances the sharpness of the bands within the gel. During electrophoresis in a discontinuous gel system, an ion gradient is formed in the early stage of electrophoresis that causes all of the proteins to focus on a single sharp band in a process called isotachophoresis. Separation of the proteins by size is achieved in the lower, "resolving" region of the gel. The resolving gel typically has a much smaller pore size, which leads to a sieving effect that now determines the electrophoretic mobility of the proteins.


title: Gel electrophoresis

text: After the electrophoresis is complete, the molecules in the gel can be stained to make them visible. DNA may be visualized using ethidium bromide which, when intercalated into DNA, fluoresce under ultraviolet light, while protein may be visualised using silver stain or Coomassie brilliant blue dye. Other methods may also be used to visualize the separation of the mixture's components on the gel. If the molecules to be separated contain radioactivity, for example in a DNA sequencing gel, an autoradiogram can be recorded of the gel. Photographs can be taken of gels, often using a Gel Doc system.


title: Gel electrophoresis

text: After separation, an additional separation method may then be used, such as isoelectric focusing or SDS-PAGE. The gel will then be physically cut, and the protein complexes extracted from each portion separately. Each extract may then be analysed, such as by peptide mass fingerprinting or de novo peptide sequencing after in-gel digestion. This can provide a great deal of information about the identities of the proteins in a complex.


title: Gel electrophoresis

text: Gel electrophoresis is used in forensics, molecular biology, genetics, microbiology and biochemistry. The results can be analyzed quantitatively by visualizing the gel with UV light and a gel imaging device. The image is recorded with a computer-operated camera, and the intensity of the band or spot of interest is measured and compared against standard or markers loaded on the same gel. The measurement and analysis are mostly done with specialized software.


title: Gel electrophoresis

text: Depending on the type of analysis being performed, other techniques are often implemented in conjunction with the results of gel electrophoresis, providing a wide range of field-specific applications.


title: Gel electrophoresis

text: In the case of nucleic acids, the direction of migration, from negative to positive electrodes, is due to the naturally occurring negative charge carried by their sugar-phosphate backbone.


title: Gel electrophoresis

text: Double-stranded DNA fragments naturally behave as long rods, so their migration through the gel is relative to their size or, for cyclic fragments, their radius of gyration. Circular DNA such as plasmids, however, may show multiple bands, the speed of migration may depend on whether it is relaxed or supercoiled. Single-stranded DNA or RNA tends to fold up into molecules with complex shapes and migrate through the gel in a complicated manner based on their tertiary structure. Therefore, agents that disrupt the hydrogen bonds, such as sodium hydroxide or formamide, are used to denature the nucleic acids and cause them to behave as long rods again.


title: Gel electrophoresis

text: Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis. See the "Chain termination method" page for an example of a polyacrylamide DNA sequencing gel. Characterization through ligand interaction of nucleic acids or fragments may be performed by mobility shift affinity electrophoresis.


title: Gel electrophoresis

text: Electrophoresis of RNA samples can be used to check for genomic DNA contamination and also for RNA degradation. RNA from eukaryotic organisms shows distinct bands of 28s and 18s rRNA, the 28s band being approximately twice as intense as the 18s band. Degraded RNA has less sharply defined bands, has a smeared appearance, and the intensity ratio is less than 2:1.


title: Gel electrophoresis

text: Proteins, unlike nucleic acids, can have varying charges and complex shapes, therefore they may not migrate into the polyacrylamide gel at similar rates, or all when placing a negative to positive EMF on the sample. Proteins, therefore, are usually denatured in the presence of a detergent such as sodium dodecyl sulfate (SDS) that coats the proteins with a negative charge. Generally, the amount of SDS bound is relative to the size of the protein (usually 1.4g SDS per gram of protein), so that the resulting denatured proteins have an overall negative charge, and all the proteins have a similar charge-to-mass ratio. Since denatured proteins act like long rods instead of having a complex tertiary shape, the rate at which the resulting SDS coated proteins migrate in the gel is relative only to their size and not their charge or shape.


title: Gel electrophoresis

text: Proteins are usually analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), by native gel electrophoresis, by preparative gel electrophoresis (QPNC-PAGE), or by 2-D electrophoresis.


title: Gel electrophoresis

text: Characterization through ligand interaction may be performed by electroblotting or by affinity electrophoresis in agarose or by capillary electrophoresis as for estimation of binding constants and determination of structural features like glycan content through lectin binding.


title: Gel electrophoresis

text: A novel application for gel electrophoresis is to separate or characterize metal or metal oxide nanoparticles (e.g. Au, Ag, ZnO, SiO2) regarding the size, shape, or surface chemistry of the nanoparticles. The scope is to obtain a more homogeneous sample (e.g. narrower particle size distribution), which then can be used in further products/processes (e.g. self-assembly processes). For the separation of nanoparticles within a gel, the particle size about the mesh size is the key parameter, whereby two migration mechanisms were identified: the unrestricted mechanism, where the particle size « mesh size, and the restricted mechanism, where particle size is similar to mesh size.


title: Gel electrophoresis

text: A 1959 book on electrophoresis by Milan Bier cites references from the 1800s. However, Oliver Smithies made significant contributions. Bier states: "The method of Smithies ... is finding wide application because of its unique separatory power." Taken in context, Bier clearly implies that Smithies' method is an improvement.


title: Tommy Vext

text: Thomas Cummings (born April 15, 1982), better known under the stage name Tommy Vext, is an American heavy metal singer and songwriter known as the former lead vocalist of Bad Wolves, Divine Heresy, and Westfield Massacre. As a solo act, he goes by The Lone Wolf.


title: Tommy Vext

text: Vext began his music career in Brooklyn, New York as a teenager singing in local hardcore bands and engaging in freestyle rap battles with the neighborhood's preeminent hopefuls. Being too young, Vext had to sneak into clubs even for his own shows. In 1996, he formed the band Maniacal Disciple with friends Jim Donovan, Steve Perlmutter, and Mike Kontaras, eventually changing their name to "Vext" (a word he found in Wu-Tang Clan's lyrics), which eventually became his nickname after he started to be known as "Tommy Vext" in the New York hardcore scene. Vext was a local buzz band, playing shows at CBGB, The Continental, and Lamours. They also opened for Biohazard, Candiria, and many others. In 2005, at the Roadrunner Records 25th-anniversary show, Vext was invited to sing with Corey Taylor of Slipknot.