V ( D ) J recombination - wikipedia V ( D ) J recombination for the European car classification category please see D - segment V ( D ) J recombination is the unique mechanism of genetic recombination that occurs only in developing lymphocytes during the early stages of T and B cell maturation. It involves somatic recombination, and results in the highly diverse repertoire of antibodies / immunoglobulins ( Igs ) and T cell receptors ( TCRs ) found on B cells and T cells, respectively. The process is a defining feature of the adaptive immune system and its development was a key event in the evolution of jawed vertebrates. V ( D ) J recombination occurs in the primary lymphoid organs ( bone marrow for B cells and thymus for T cells ) and in a nearly random fashion rearranges variable ( V ), joining ( J ), and in some cases, diversity ( D ) gene segments. The process ultimately results in novel amino acid sequences in the antigen - binding regions of Igs and TCRs that allow for the recognition of antigens from nearly all pathogens including bacteria, viruses, parasites, and worms as well as `` altered self cells '' as seen in cancer. The recognition can also be allergic in nature ( e.g., to pollen or other allergens ) or may be `` autoreactive '' and lead to autoimmunity. In 1987, Susumu Tonegawa was awarded the Nobel Prize in Physiology or Medicine `` for his discovery of the genetic principle for generation of antibody diversity ''. Contents 1 Background 2 Immunoglobulins 2.1 Heavy chain 2.2 Light chain 3 T cell receptors 4 Mechanism 4.1 Key enzymes and components 4.2 Process 5 See also 6 References 7 Further reading Background Human antibody molecules ( and B cell receptors ) are composed of heavy and light chains ( each of which contains both constant ( C ) and variable ( V ) regions ), which are encoded by genes on three loci : The immunoglobulin heavy locus ( IGH @ ) on chromosome 14, containing the gene segments for the immunoglobulin heavy chain. The immunoglobulin kappa ( κ ) locus ( IGK @ ) on chromosome 2, containing the gene segments for part of the immunoglobulin light chain. The immunoglobulin lambda ( λ ) locus ( IGL @ ) on chromosome 22, containing the gene segments for the remainder of the immunoglobulin light chain. Each heavy chain and light chain gene contains multiple copies of three different types of gene segments for the variable regions of the antibody proteins. For example, the human immunoglobulin heavy chain region contains 2 Constant ( Cμ and Cδ ) gene segments and 44 Variable ( V ) gene segments, plus 27 Diversity ( D ) gene segments and 6 Joining ( J ) gene segments. The light chains also possess 2 Constant ( Cμ and Cδ ) gene segments and numerous V and J gene segments, but do not have D gene segments. DNA rearrangement causes one copy of each type of gene segment to go in any given lymphocyte, generating an enormous antibody repertoire ; roughly 3 × 10 combinations are possible, although some are removed due to self reactivity. Most T cell receptors are composed of an alpha chain and a beta chain. The T cell receptor genes are similar to immunoglobulin genes in that they too contain multiple V, D and J gene segments in their beta chains ( and V and J gene segments in their alpha chains ) that are rearranged during the development of the lymphocyte to provide that cell with a unique antigen receptor. The T cell receptor in this sense is the topological equivalent to an antigen - binding fragment of the antibody, both being part of the immunoglobulin superfamily. Failure of the cell to create a successful product that does not self - react leads to apoptosis. Autoimmunity is prevented by eliminating lymphocytes that self - react in the thymus by testing them against an array of self antigens expressed through the function of Aire. Interestingly, the immunoglobulin lambda light chain locus contains protein - coding genes that can be lost with its rearrangement. This is based on a physiological mechanism and is not pathogenetic for leukemias or lymphomas. Immunoglobulins Simplistic overview of V ( D ) J recombination of immunoglobulin heavy chains Heavy chain In the developing B cell, the first recombination event to occur is between one D and one J gene segment of the heavy chain locus. Any DNA between these two gene segments is deleted. This D-J recombination is followed by the joining of one V gene segment, from a region upstream of the newly formed DJ complex, forming a rearranged VDJ gene segment. All other gene segments between V and D segments are now deleted from the cell 's genome. Primary transcript ( unspliced RNA ) is generated containing the VDJ region of the heavy chain and both the constant mu and delta chains ( C and C ). ( i.e. the primary transcript contains the segments : V-D-J-C - C ). The primary RNA is processed to add a polyadenylated ( poly - A ) tail after the C chain and to remove sequence between the VDJ segment and this constant gene segment. Translation of this mRNA leads to the production of the Ig M heavy chain protein. Light chain The kappa ( κ ) and lambda ( λ ) chains of the immunoglobulin light chain loci rearrange in a very similar way, except that the light chains lack a D segment. In other words, the first step of recombination for the light chains involves the joining of the V and J chains to give a VJ complex before the addition of the constant chain gene during primary transcription. Translation of the spliced mRNA for either the kappa or lambda chains results in formation of the Ig κ or Ig λ light chain protein. Interestingly, the immunoglobulin lambda light chain locus contains protein - coding genes that can be lost with its rearrangement. This is based on a physiological mechanism and is not pathogenetic for leukemias or lymphomas. However, the rearrangement of several lambda variable subgenes can activate expression of an overlapping miRNA gene, which has consequences for gene expression regulation. Assembly of the Ig μ heavy chain and one of the light chains results in the formation of membrane bound form of the immunoglobulin IgM that is expressed on the surface of the immature B cell. T cell receptors During thymocyte development, the T cell receptor ( TCR ) chains undergo essentially the same sequence of ordered recombination events as that described for immunoglobulins. D - to - J recombination occurs first in the β chain of the TCR. This process can involve either the joining of the D 1 gene segment to one of six J 1 segments or the joining of the D 2 gene segment to one of six J 2 segments. DJ recombination is followed ( as above ) with V - to - D J rearrangements. All gene segments between the V - D - J gene segments in the newly formed complex are deleted and the primary transcript is synthesized that incorporates the constant domain gene ( V - D - J - C ). mRNA transcription splices out any intervening sequence and allows translation of the full length protein for the TCR C chain. The rearrangement of the alpha ( α ) chain of the TCR follows β chain rearrangement, and resembles V - to - J rearrangement described for Ig light chains ( see above ). The assembly of the β - and α - chains results in formation of the αβ - TCR that is expressed on a majority of T cells. Mechanism Key enzymes and components The process of V ( D ) J recombination is mediated by VDJ recombinase, which is a diverse collection of enzymes. The key enzymes involved are recombination activating genes 1 and 2 ( RAG ), terminal deoxynucleotidyl transferase ( TdT ), and Artemis nuclease, a member of the ubiquitous non-homologous end joining ( NHEJ ) pathway for DNA repair. Several other enzymes are known to be involved in the process and include DNA - dependent protein kinase ( DNA - PK ), X-ray repair cross-complementing protein 4 ( XRCC4 ), DNA ligase IV, non-homologous end - joining factor 1 ( NHEJ1 ; also known as Cernunnos or XRCC4 - like factor ( XLF ) ), the recently discovered Paralog of XRCC4 and XLF ( PAXX ), and DNA polymerases λ and μ. Some enzymes involved are specific to lymphocytes ( e.g., RAG, TdT ), while others are found in other cell types and even ubiquitously ( e.g., NHEJ components ). To maintain the specificity of recombination, V ( D ) J recombinase recognizes and binds to Recombination Signal Sequences ( RSSs ) flanking the variable ( V ), diversity ( D ), and joining ( J ) genes segments. RSSs are composed of three elements : a heptamer of seven conserved nucleotides, a spacer region of 12 or 23 basepairs in length, and a nonamer of nine conserved nucleotides. While the majority of RSSs vary in sequence, the consensus heptamer and nonamer sequences are CACAGTG and ACAAAAACC, respectively ; and although the sequence of the spacer region is poorly conserved, the length is highly conserved. The length of the spacer region corresponds to approximately one ( 12 basepairs ) or two turns ( 23 basepairs ) of the DNA helix. Following what is known as the 12 / 23 Rule, gene segments to be recombined are usually adjacent to RSSs of different spacer lengths ( i.e., one has a `` 12RSS '' and one has a `` 23RSS '' ). This is an important feature in the regulation of V ( D ) J recombination. Process V ( D ) J recombination begins when V ( D ) J recombinase ( through the activity of RAG1 ) binds an RSS flanking a coding gene segment ( V, D, or J ) and creates a single - strand nick in the DNA between the first base of the RSS ( just before the heptamer ) and the coding segment. This is essentially energetically neutral ( no need for ATP hydrolysis ) and results in the formation of a free 3 ' hydroxyl group and a 5 ' phosphate group on the same strand. The reactive hydroxyl group is positioned by the recombinase to attack the phosphodiester bond of opposite strand, forming two DNA ends : a hairpin ( stem - loop ) on the coding segment and a blunt end on the signal segment. The current model is that DNA nicking and hairpin formation occurs on both strands simultaneously ( or nearly so ) in a complex known as a recombination center. The blunt signal ends are flushly ligated together to form a circular piece of DNA containing all of the intervening sequences between the coding segments known as a signal joint ( although circular in nature, this is not to be confused with a plasmid ). While originally thought to be lost during successive cell divisions, there is evidence that signal joints may re-enter the genome and lead to pathologies by activating oncogenes or interrupting tumor suppressor gene function ( s ) ( Ref ). The coding ends are processed further prior to their ligation by several events that ultimately lead to junctional diversity. Processing begins when DNA - PK binds to each broken DNA end and recruits several other proteins including Artemis, XRCC4, DNA ligase IV, Cernunnos, and several DNA polymerases. DNA - PK forms a complex that leads to its autophosphorylation, resulting in activation of Artemis. The coding end hairpins are opened by the activity of Artemis. If they are opened at the center, a blunt DNA end will result ; however in many cases, the opening is `` off - center '' and results in extra bases remaining on one strand ( an overhang ). These are known as palindromic ( P ) nucleotides due to the palindromic nature of the sequence produced when DNA repair enzymes resolve the overhang. The process of hairpin opening by Artemis is a crucial step of V ( D ) J recombination and is defective in the severe combined immunodeficiency ( scid ) mouse model. Next, XRCC4, Cernunnos, and DNA - PK align the DNA ends and recruit terminal deoxynucleotidyl transferase ( TdT ), a template - independent DNA polymerase that adds non-templated ( N ) nucleotides to the coding end. The addition is mostly random, but TdT does exhibit a preference for G / C nucleotides. As with all known DNA polymerases, the TdT adds nucleotides to one strand in a 5 ' to 3 ' direction. Lastly, exonucleases can remove bases from the coding ends ( including any P or N nucleotides that may have formed ). DNA polymerases λ and μ then insert additional nucleotides as needed to make the two ends compatible for joining. This is a stochastic process, therefore any combination of the addition of P and N nucleotides and exonucleolytic removal can occur ( or none at all ). Finally, the processed coding ends are ligated together by DNA ligase IV. All of these processing events result in an antigen - binding region that is highly variable, even when the same gene segments are recombined. V ( D ) J recombination allows for the generation of immunoglobulins and T cell receptors to antigens that neither the organism nor its ancestor ( s ) need to have previously encountered, allowing for an adaptive immune response to novel pathogens that develop or to those that frequently change ( e.g., seasonal influenza ). However, a major caveat to this process is that the DNA sequence must remain in - frame in order to maintain the correct amino acid sequence in the final protein product. If the resulting sequence is out - of - frame, the development of the cell will be arrested, and the cell will not survive to maturity. V ( D ) J recombination is therefore a very costly process that must be ( and is ) strictly regulated and controlled. See also Antibody B cell B cell receptor T cell T cell receptor Recombination - activating gene NKT cell References Jump up ^ `` The Nobel Prize in Physiology or Medicine 1987 ''. nobelprize.org. Retrieved 26 December 2014. 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Nature Reviews Immunology. 11 ( 4 ) : 251 -- 263. doi : 10.1038 / nri2941. PMID 21394103. Jump up ^ Curry, John ; Geier, Jamie ; Schlissel, Mark ( 2005 ). `` Single - Strand Recombination Signal Sequence Nicks in vivo : Evidence for a Capture Model of Synapsis ''. Nature Immunology. 6 ( 12 ) : 1272 -- 1279. doi : 10.1038 / ni1270. PMID 16286921. Jump up ^ Agrawal, Alka ; Schatz, David ( 1997 ). `` RAG1 and RAG2 Form a Stable Postcleavage Synaptic Complex with DNA Containing Signal Ends in V ( D ) J Recombination ''. Cell. 89 ( 1 ) : 43 -- 53. doi : 10.1016 / s0092 - 8674 ( 00 ) 80181 - 6. PMID 9094713. Jump up ^ Fugmann, Sebastian ; Lee, AIfred ; Shockett, Penny ; Villey, Isabelle ; Schatz, David ( 2000 ). `` The RAG Proteins and V ( D ) J Recombination : Complexes, Ends, and Transposition ''. Annual Review of Immunology. 18 : 495 -- 527. doi : 10.1146 / annurev. immunol. 18.1. 495. PMID 10837067. Jump up ^ Lewis, Susanna ( 1994 ). `` The Mechanism of V ( D ) J Joining : Lessons from Molecular, Immunological, and Comparative Analyses ''. Advances in Immunology. 56 : 27 -- 150. doi : 10.1016 / s0065 - 2776 ( 08 ) 60450 - 2. PMID 8073949. Jump up ^ Helmink, Beth ; Sleckman, Barry ( 2012 ). `` The response to and repair of RAG - mediated DNA double - strand breaks ''. Annual Review of Immunology. 30 : 175 -- 202. doi : 10.1146 / annurev - immunol - 030409 - 101320. PMC 4038028. PMID 22224778. Jump up ^ Ma, Yunmei ; Schwarz, Klaus ; Lieber, Michael ( 2005 ). `` The Artemis : DNA - PKcs Endonuclease Cleaves DNA Loops, Flaps, and Gaps ''. DNA Repair. 4 ( 7 ) : 845 -- 851. doi : 10.1016 / j. dnarep. 2005.04. 013. PMID 15936993. Jump up ^ Lu, Haihui ; Schwarz, Klaus ; Lieber, Michael ( 2007 ). `` Extent to Which Hairpin Opening by the Artemis : DNA - PKcs Complex can Contribute to Junctional Diversity in V ( D ) J Recombination ''. Nucleic Acids Research. 35 ( 20 ) : 6917 -- 6923. doi : 10.1093 / nar / gkm823. PMC 2175297. PMID 17932067. Jump up ^ Gauss, George ; Lieber, Michael ( 1996 ). `` Mechanistic Constraints on Diversity in Human V ( D ) J Recombination ''. Molecular and Cellular Biology. 16 ( 1 ) : 258 -- 269. PMC 230999. PMID 8524303. Jump up ^ Benedict, Cindy ; Gilfillan, Susan ; Thai, To - Ha ; Kearney, John ( 2000 ). `` Terminal Deoxynucleotidyl Transferase and Repertoire Development ''. Immunological Reviews. 175 : 150 -- 157. doi : 10.1111 / j. 1600 - 065x. 2000. imr017518. x. PMID 10933600. Jump up ^ D.C. van Gent & M. van der Burg ( 2007 ). `` Non-Homologous End - Joining, a Sticky Affair ''. Oncogene. 26 ( 56 ) : 7731 -- 7740. doi : 10.1038 / sj. onc. 1210871. PMID 18066085. Further reading Hartwell LH, Hood L, Goldberg ML, Reynolds AE, Silver LM, Veres RC ( 2000 ). Chapter 24, Evolution at the molecular level. In : Genetics. New York : McGraw - Hill. pp. 805 -- 807. ISBN 0 - 07 - 299587 - 4. V ( D ) J Recombination. Series : Advances in Experimental Medicine and Biology, Vol. 650 Ferrier, Pierre ( Ed. ) Landes Bioscience 2009, XII, 199 p. ISBN 978 - 1 - 4419 - 0295 - 5 Immunology : lymphocytic adaptive immune system and complement Lymphoid Antigens Antigen Superantigen Allergen Hapten Epitope Linear Conformational Mimotope Antigen presentation / Professional APCs : Dendritic cell Macrophage B cell Immunogen Antibodies Antibody Monoclonal antibodies Polyclonal antibodies Autoantibody Microantibody Polyclonal B cell response Allotype Isotype Idiotype Immune complex Paratope Immunity vs. tolerance action : Immunity Autoimmunity Alloimmunity Allergy Hypersensitivity Inflammation Cross-reactivity inaction : Tolerance Central Peripheral Clonal anergy Clonal deletion Tolerance in pregnancy Immunodeficiency Immunogenetics Affinity maturation Somatic hypermutation Clonal selection V ( D ) J recombination Junctional diversity Immunoglobulin class switching MHC / HLA Lymphocytes Cellular T cell Humoral B cell NK cell Substances Cytokines Opsonin Cytolysin Retrieved from `` https://en.wikipedia.org/w/index.php?title=V(D)J_recombination&oldid=821620731 '' Categories : Immune system Lymphocytes Immunology Talk Contents About Wikipedia Català Čeština Deutsch Español Français Galego Italiano 日本 語 Polski Português Русский Српски / srpski Srpskohrvatski / српскохрватски தமிழ் Türkçe Українська 中文 Edit links This page was last edited on 21 January 2018, at 16 : 35. 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Question: which gene segments code for the variable region of the heavy chain
Answer: V