Fluorescence - wikipedia Fluorescence Not to be confused with Inflorescence. For the album by Asobi Seksu, see Fluorescence ( album ). Fluorescent minerals emit visible light when exposed to ultraviolet light. Biofluorescent marine organisms Willemite and calcite in UV light Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation. The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, while the emitted light is in the visible region, which gives the fluorescent substance a distinct color that can only be seen when exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescence, where they continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors ( fluorescence spectroscopy ), fluorescent labelling, dyes, biological detectors, cosmic - ray detection, and, most commonly, fluorescent lamps. Fluorescence also occurs frequently in nature in some minerals and in various biological states in many branches of the animal kingdom. Contents 1 History 2 Physical principles 2.1 Photochemistry 2.2 Quantum yield 2.3 Lifetime 2.4 Jablonski diagram 2.5 Fluorescence anisotropy 2.6 Fluorence 3 Rules 3.1 Kasha 's rule 3.2 Mirror image rule 3.3 Stokes shift 4 Fluorescence in nature 4.1 Biofluorescence vs. bioluminescence vs. biophosphorescence 4.1. 1 Biofluorescence 4.1. 2 Bioluminescence 4.1. 3 Biophosphorescence 4.2 Mechanisms of biofluorescence 4.2. 1 Epidermal chromatophores 4.3 Phylogenetics 4.3. 1 Evolutionary origins 4.3. 2 Adaptive functions 4.4 Aquatic biofluorescence 4.4. 1 Photic zone 4.4. 1.1 Fish 4.4. 1.2 Coral 4.4. 1.3 Cephalopods 4.4. 1.4 Jellyfish 4.4. 1.5 Mantis shrimp 4.4. 2 Aphotic zone 4.4. 2.1 Siphonophores 4.4. 2.2 Dragonfish 4.5 Terrestrial biofluorescence 4.5. 1 Amphibians 4.5. 2 Butterflies 4.5. 3 Parrots 4.5. 4 Arachnids 4.5. 5 Plants 4.6 Abiotic fluorescence 4.6. 1 Gemology, mineralogy and geology 4.6. 2 Organic liquids 4.6. 3 Atmosphere 4.6. 4 Common materials that fluoresce 5 Applications of fluorescence 5.1 Lighting 5.2 Analytical chemistry 5.3 Spectroscopy 5.4 Biochemistry and medicine 5.4. 1 Microscopy 5.4. 2 Other techniques 5.5 Forensics 5.6 Mechanical engineering 5.7 Signage 5.8 Optical brighteners 6 See also 7 References 8 Bibliography 9 External links History Lignum nephriticum cup made from the wood of the narra tree ( Pterocarpus indicus ), and a flask containing its fluorescent solution Matlaline, the fluorescent substance in the wood of the tree Eysenhardtia polystachya An early observation of fluorescence was described in 1560 by Bernardino de Sahagún and in 1565 by Nicolás Monardes in the infusion known as lignum nephriticum ( Latin for `` kidney wood '' ). It was derived from the wood of two tree species, Pterocarpus indicus and Eysenhardtia polystachya. The chemical compound responsible for this fluorescence is matlaline, which is the oxidation product of one of the flavonoids found in this wood. In 1819, Edward D. Clarke and in 1822 René Just Haüy described fluorescence in fluorites, Sir David Brewster described the phenomenon for chlorophyll in 1833 and Sir John Herschel did the same for quinine in 1845. In his 1852 paper on the `` Refrangibility '' ( wavelength change ) of light, George Gabriel Stokes described the ability of fluorspar and uranium glass to change invisible light beyond the violet end of the visible spectrum into blue light. He named this phenomenon fluorescence : `` I am almost inclined to coin a word, and call the appearance fluorescence, from fluor - spar ( i.e., fluorite ), as the analogous term opalescence is derived from the name of a mineral. '' The name was derived from the mineral fluorite ( calcium difluoride ), some examples of which contain traces of divalent europium, which serves as the fluorescent activator to emit blue light. In a key experiment he used a prism to isolate ultraviolet radiation from sunlight and observed blue light emitted by an ethanol solution of quinine exposed by it. Physical principles Photochemistry Fluorescence occurs when an orbital electron of a molecule, atom, or nanostructure, relaxes to its ground state by emitting a photon from an excited singlet state : Excitation : S 0 + h ν e x → S 1 ( displaystyle S_ ( 0 ) + h \nu _ ( ex ) \to S_ ( 1 ) ) Fluorescence ( emission ) : S 1 → S 0 + h ν e m + h e a t ( displaystyle S_ ( 1 ) \to S_ ( 0 ) + h \nu _ ( em ) + heat ) Here h ν ( displaystyle h \nu ) is a generic term for photon energy with h = Planck 's constant and ν ( displaystyle \nu ) = frequency of light. The specific frequencies of exciting and emitted light are dependent on the particular system. S is called the ground state of the fluorophore ( fluorescent molecule ), and S is its first ( electronically ) excited singlet state. A molecule in S can relax by various competing pathways. It can undergo non-radiative relaxation in which the excitation energy is dissipated as heat ( vibrations ) to the solvent. Excited organic molecules can also relax via conversion to a triplet state, which may subsequently relax via phosphorescence, or by a secondary non-radiative relaxation step. Relaxation from S can also occur through interaction with a second molecule through fluorescence quenching. Molecular oxygen ( O ) is an extremely efficient quencher of fluorescence just because of its unusual triplet ground state. In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation ; this phenomenon is known as the Stokes shift. However, when the absorbed electromagnetic radiation is intense, it is possible for one electron to absorb two photons ; this two - photon absorption can lead to emission of radiation having a shorter wavelength than the absorbed radiation. The emitted radiation may also be of the same wavelength as the absorbed radiation, termed `` resonance fluorescence ''. Molecules that are excited through light absorption or via a different process ( e.g. as the product of a reaction ) can transfer energy to a second ' sensitized ' molecule, which is converted to its excited state and can then fluoresce. Quantum yield The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. Φ = Number of photons emitted Number of photons absorbed ( displaystyle Phi = ( \frac ( \text ( Number of photons emitted ) ) ( \text ( Number of photons absorbed ) ) ) ) The maximum possible fluorescence quantum yield is 1.0 ( 100 % ) ; each photon absorbed results in a photon emitted. Compounds with quantum yields of 0.10 are still considered quite fluorescent. Another way to define the quantum yield of fluorescence is by the rate of excited state decay : Φ = k f ∑ i k i ( displaystyle Phi = ( \frac ( ( k ) _ ( f ) ) ( sum _ ( i ) ( k ) _ ( i ) ) ) ) where k f ( displaystyle ( k ) _ ( f ) ) is the rate constant of spontaneous emission of radiation and ∑ i k i ( displaystyle sum _ ( i ) ( k ) _ ( i ) ) is the sum of all rates of excited state decay. Other rates of excited state decay are caused by mechanisms other than photon emission and are, therefore, often called `` non-radiative rates '', which can include : dynamic collisional quenching, near - field dipole - dipole interaction ( or resonance energy transfer ), internal conversion, and intersystem crossing. Thus, if the rate of any pathway changes, both the excited state lifetime and the fluorescence quantum yield will be affected. Fluorescence quantum yields are measured by comparison to a standard. The quinine salt quinine sulfate in a sulfuric acid solution is a common fluorescence standard. Lifetime Jablonski diagram. After an electron absorbs a high - energy photon the system is excited electronically and vibrationally. The system relaxes vibrationally, and eventually fluoresces at a longer wavelength. The fluorescence lifetime refers to the average time the molecule stays in its excited state before emitting a photon. Fluorescence typically follows first - order kinetics : ( S 1 ) = ( S 1 ) 0 e − Γ t ( displaystyle left ( S_ ( 1 ) \right ) = left ( S_ ( 1 ) \right ) _ ( 0 ) e ^ ( - Gamma t ) ) where ( S 1 ) ( displaystyle left ( S_ ( 1 ) \right ) ) is the concentration of excited state molecules at time t ( displaystyle t ), ( S 1 ) 0 ( displaystyle left ( S_ ( 1 ) \right ) _ ( 0 ) ) is the initial concentration and Γ ( displaystyle Gamma ) is the decay rate or the inverse of the fluorescence lifetime. This is an instance of exponential decay. Various radiative and non-radiative processes can de-populate the excited state. In such case the total decay rate is the sum over all rates : Γ t o t = Γ r a d + Γ n r a d ( displaystyle Gamma _ ( tot ) = Gamma _ ( rad ) + Gamma _ ( nrad ) ) where Γ t o t ( displaystyle Gamma _ ( tot ) ) is the total decay rate, Γ r a d ( displaystyle Gamma _ ( rad ) ) the radiative decay rate and Γ n r a d ( displaystyle Gamma _ ( nrad ) ) the non-radiative decay rate. It is similar to a first - order chemical reaction in which the first - order rate constant is the sum of all of the rates ( a parallel kinetic model ). If the rate of spontaneous emission, or any of the other rates are fast, the lifetime is short. For commonly used fluorescent compounds, typical excited state decay times for photon emissions with energies from the UV to near infrared are within the range of 0.5 to 20 nanoseconds. The fluorescence lifetime is an important parameter for practical applications of fluorescence such as fluorescence resonance energy transfer and fluorescence - lifetime imaging microscopy. Jablonski diagram The Jablonski diagram describes most of the relaxation mechanisms for excited state molecules. The diagram alongside shows how fluorescence occurs due to the relaxation of certain excited electrons of a molecule. Fluorescence anisotropy Fluorophores are more likely to be excited by photons if the transition moment of the fluorophore is parallel to the electric vector of the photon. The polarization of the emitted light will also depend on the transition moment. The transition moment is dependent on the physical orientation of the fluorophore molecule. For fluorophores in solution this means that the intensity and polarization of the emitted light is dependent on rotational diffusion. Therefore, anisotropy measurements can be used to investigate how freely a fluorescent molecule moves in a particular environment. Fluorescence anisotropy can be defined quantitatively as r = I ∥ − I ⊥ I ∥ + 2 I ⊥ ( displaystyle r = ( I_ ( parallel ) - I_ ( perp ) over I_ ( parallel ) + 2I_ ( perp ) ) ) where I ∥ ( displaystyle I_ ( parallel ) ) is the emitted intensity parallel to polarization of the excitation light and I ⊥ ( displaystyle I_ ( perp ) ) is the emitted intensity perpendicular to the polarization of the excitation light. Fluorence Strongly fluorescent pigments often have an unusual appearance which is often described colloquially as a `` neon color. '' This phenomenon was termed `` Farbenglut '' by Hermann von Helmholtz and `` fluorence '' by Ralph M. Evans. It is generally thought to be related to the high brightness of the color relative to what it would be as a component of white. Fluorescence shifts energy in the incident illumination from shorter wavelengths to longer ( such as blue to yellow ) and thus can make the fluorescent color appear brighter ( more saturated ) than it could possibly be by reflection alone. Rules There are several general rules that deal with fluorescence. Each of the following rules has exceptions but they are useful guidelines for understanding fluorescence ( these rules do not necessarily apply to two - photon absorption ). Kasha 's rule Kasha 's rule dictates that the quantum yield of luminescence is independent of the wavelength of exciting radiation. This occurs because excited molecules usually decay to the lowest vibrational level of the excited state before fluorescence emission takes place. The Kasha -- Vavilov rule does not always apply and is violated severely in many simple molecules. A somewhat more reliable statement, although still with exceptions, would be that the fluorescence spectrum shows very little dependence on the wavelength of exciting radiation. Mirror image rule For many fluorophores the absorption spectrum is a mirror image of the emission spectrum. This is known as the mirror image rule and is related to the Franck -- Condon principle which states that electronic transitions are vertical, that is energy changes without distance changing as can be represented with a vertical line in Jablonski diagram. This means the nucleus does not move and the vibration levels of the excited state resemble the vibration levels of the ground state. Stokes shift Main article : Stokes shift In general, emitted fluorescence light has a longer wavelength and lower energy than the absorbed light. This phenomenon, known as Stokes shift, is due to energy loss between the time a photon is absorbed and when a new one is emitted. The causes and magnitude of Stokes shift can be complex and are dependent on the fluorophore and its environment. However, there are some common causes. It is frequently due to non-radiative decay to the lowest vibrational energy level of the excited state. Another factor is that the emission of fluorescence frequently leaves a fluorophore in a higher vibrational level of the ground state. Fluorescence in nature There are many natural compounds that exhibit fluorescence, and they have a number of applications. Some deep - sea animals, such as the greeneye, use fluorescence. Biofluorescence vs. bioluminescence vs. biophosphorescence Biofluorescence Biofluorescence is the absorption of electromagnetic wavelengths from the visible light spectrum by fluorescent proteins in a living organism, and the emission of light at a lower energy level. This causes the light that is emitted to be a different color than the light that is absorbed. Stimulating light excites an electron, raising energy to an unstable level. This instability is unfavorable, so the energized electron is returned to a stable state almost as immediately as it becomes unstable. This return to stability corresponds with the release of excess energy in the form of fluorescence light. This emission of light is only observable when the stimulant light is still providing light to the organism / object and is typically yellow, pink, orange, red, green, or purple. Biofluorescence is often confused with the following forms of biotic light, bioluminescence and biophosphorescence. Bioluminescence Bioluminescence differs from biofluorescence in that it is the natural production of light by chemical reactions within an organism, whereas biofluorescence is the absorption and reemission of light from the environment. Biophosphorescence Biophosphorescence is similar to biofluorescence in its requirement of light wavelengths as a provider of excitation energy. The difference here lies in the relative stability of the energized electron. Unlike with biofluorescence, here the electron retains stability, emitting light that continues to `` glow - in - the - dark '' even long after the stimulating light source has been removed. Mechanisms of biofluorescence Epidermal chromatophores Pigment cells that exhibit fluorescence are called fluorescent chromatophores, and function somatically similar to regular chromatophores. These cells are dendritic, and contain pigments called fluorosomes. These pigments contain fluorescent proteins which are activated by K+ ( potassium ) ions, and it is their movement, aggregation, and dispersion within the fluorescent chromatophore that cause directed fluorescence patterning. Fluorescent cells are innervated the same as other chromatphores, like melanophores, pigment cells that contain melanin. Short term fluorescent patterning and signaling is controlled by the nervous system. Fluorescent chromatophores can be found in the skin ( e.g. in fish ) just below the epidermis, amongst other chromatophores. Epidermal fluorescent cells in fish also respond to hormonal stimuli by the α -- MSH and MCH hormones much the same as melanophores. This suggests that fluorescent cells may have color changes throughout the day that coincide with their circadian rhythm. Fish may also be sensitive to cortisol induced stress responses to environmental stimuli, such as interaction with a predator or engaging in a mating ritual. Phylogenetics Evolutionary origins It is suspected by some scientists that GFPs and GFP like proteins began as electron donors activated by light. These electrons were then used for reactions requiring light energy. Functions of fluorescent proteins, such as protection from the sun, conversion of light into different wavelengths, or for signaling are thought to have evolved secondarily. Fluorescence has multiple origins in the tree of life. This diagram displays the origins within actinopterygians ( ray finned fish ). The incidence of fluorescence across the tree of life is widespread, and has been studied most extensively in a phylogenetic sense in fish. The phenomenon appears to have evolved multiple times in multiple taxa such as in the anguilliformes ( eels ), gobioidei ( gobies and cardinalfishes ), and tetradontiformes ( triggerfishes ), along with the other taxa discussed later in the article. Fluorescence is highly genotypically and phenotypically variable even within ecosystems, in regards to the wavelengths emitted, the patterns displayed, and the intensity of the fluorescence. Generally, the species relying upon camouflage exhibit the greatest diversity in fluorescence, likely because camouflage is one of the most common uses of fluorescence. Adaptive functions Currently, relatively little is known about the functional significance of fluorescence and fluorescent proteins. However, it is suspected that biofluorescence may serve important functions in signaling and communication, mating, lures, camouflage, UV protection and antioxidation, photoacclimation, dinoflagellate regulation, and in coral health. Aquatic biofluorescence Water absorbs light of long wavelengths, so less light from these wavelengths reflects back to reach the eye. Therefore, warm colors from the visual light spectrum appear less vibrant at increasing depths. Water scatters light of shorter wavelengths, meaning cooler colors dominate the visual field in the photic zone. Light intensity decreases 10 fold with every 75 m of depth, so at depths of 75 m, light is 10 % as intense as it is on the surface, and is only 1 % as intense at 150 m as it is on the surface. Because the water filters out the wavelengths and intensity of water reaching certain depths, different proteins, because of the wavelengths and intensities of light they are capable of absorbing, are better suited to different depths. Theoretically, some fish eyes can detect light as deep as 1000 m. At these depths of the aphotic zone, the only sources of light are organisms themselves, giving off light through chemical reactions in a process called bioluminescence. Fluorescence is simply defined as the absorption of electromagnetic radiation at one wavelength and its reemission at another, lower energy wavelength. Thus any type of fluorescence depends on the presence of external sources of light. Biologically functional fluorescence is found in the photic zone, where there is not only enough light to cause biofluorescence, but enough light for other organisms to detect it. The visual field in the photic zone is naturally blue, so colors of fluorescence can be detected as bright reds, oranges, yellows, and greens. Green is the most commonly found color in the biofluorescent spectrum, yellow the second most, orange the third, and red is the rarest. Fluorescence can occur in organisms in the aphotic zone as a byproduct of that same organism 's bioluminescence. Some biofluorescence in the aphotic zone is merely a byproduct of the organism 's tissue biochemistry and does not have a functional purpose. However, some cases of functional and adaptive significance of biofluorescence in the aphotic zone of the deep ocean is an active area of research. Photic zone Fish Fluorescent marine fish Bony fishes living in shallow water, due to living in a colorful environment, generally have good color vision. Thus, in shallow - water fishes, red, orange, and green fluorescence most likely serves as a means of communication with conspecifics, especially given the great phenotypic variance of the phenomenon. Many fish that exhibit biofluorescence, such as sharks, lizardfish, scorpionfish, wrasses, and flatfishes, also possess yellow intraocular filters. Yellow intraocular filters in the lenses and cornea of certain fishes function as long - pass filters, thus enabling the species that possess them to visualize and potentially exploit fluorescence to enhance visual contrast and patterns that are unseen to other fishes and predators that lack this visual specialization. Fishes that possess the necessary yellow intraocular filters for visualizing biofluorescence potentially exploit a light signal from members of it or a similar functional role. Biofluorescent patterning was especially prominent in cryptically patterned fishes possessing complex camouflage, and that many of these lineages also possess yellow long - pass intraocular filters that could enable visualization of such patterns. Another adaptive use of fluorescence is to generate red light from the ambient blue light of the photic zone to aid vision. Red light can only be seen across short distances due to attenuation of red light wavelengths by water. Many fish species that fluoresce are small, group - living, or benthic / aphotic, and have conspicuous patterning. This patterning is caused by fluorescent tissue and is visible to other members of the species, however the patterning is invisible at other visual spectra. These intraspecific fluorescent patterns also coincide with intra-species signaling. The patterns present in ocular rings to indicate directionality of an individual 's gaze, and along fins to indicate directionality of an individual 's movement. Current research suspects that this red fluorescence is used for private communication between members of the same species. Due to the prominence of blue light at ocean depths, red light and light of longer wavelengths are muddled, and many predatory reef fish have little to no sensitivity for light at these wavelengths. Fish such as the fairy wrasse that have developed visual sensitivity to longer wavelengths are able to display red fluorescent signals that give a high contrast to the blue environment and are conspicuous to conspecifics in short ranges, yet are relatively invisible to other common fish that have reduced sensitivities to long wavelengths. Thus, fluorescence can be used as adaptive signaling and intra-species communication in reef fish. Additionally, it is suggested that fluorescent tissues that surround an organism 's eyes are used to convert blue light from the photic zone or green bioluminescence in the aphotic zone into red light to aid vision. Coral Fluorescence serves a wide variety of functions in coral. Fluorescent proteins in corals may contribute to photosynthesis by converting otherwise unusable wavelengths of light into ones for which the coral 's symbiotic algae are able to conduct photosynthesis. Also, the proteins may fluctuate in number as more or less light becomes available as a means of photoacclimation. Similarly, these fluorescent proteins may possess antioxidant capacities to eliminate oxygen radicals produced by photosynthesis. Finally, through modulating photosynthesis, the fluorescent proteins may also serve as a means of regulating the activity of the coral 's photosynthetic algal symbionts. Cephalopods Alloteuthis subulata and Loligo vulgaris, two types of nearly transparent squid, have fluorescent spots above their eyes. These spots reflect incident light, which may serve as a means of camouflage, but also for signaling to other squids for schooling purposes. Jellyfish Aequoria victoria, biofluorescent jellyfish known for GFP Another, well - studied example of biofluorescence in the ocean is the hydrozoan Aequorea victoria. This jellyfish lives in the photic zone off the west coast of North America and was identified as a carrier of green fluorescent protein ( GFP ) by Osamu Shimomura. The gene for these green fluorescent proteins has been isolated and is scientifically significant because it is widely used in genetic studies to indicate the expression of other genes. Mantis shrimp Several species of mantis shrimp, which are stomatopod crustaceans, including Lysiosquillina glabriuscula, have yellow fluorescent markings along their antennal scales and carapace ( shell ) that males present during threat displays to predators and other males. The display involves raising the head and thorax, spreading the striking appendages and other maxillipeds, and extending the prominent, oval antennal scales laterally, which makes the animal appear larger and accentuates its yellow fluorescent markings. Furthermore, as depth increases, mantis shrimp fluorescence accounts for a greater part of the visible light available. During mating rituals, mantis shrimp actively fluoresce, and the wavelength of this fluorescence matches the wavelengths detected by their eye pigments. Aphotic zone Siphonophores Siphonophorae is an order of marine animals from the phylum Hydrozoa that consist of a specialized medusoid and polyp zooid. Some siphonophores, including the genus Erenna that live in the aphotic zone between depths of 1600 m and 2300 m, exhibit yellow to red fluorescence in the photophores of their tentacle - like tentilla. This fluorescence occurs as a by - product of bioluminescence from these same photophores. The siphonophores exhibit the fluorescence in a flicking pattern that is used as a lure to attract prey. Dragonfish The predatory deep - sea dragonfish Malacosteus niger, the closely related Aristostomias genus and the species Pachystomias microdon are capable of harnessing the blue light emitted from their own bioluminescence to generate red biofluorescence from suborbital photophores. This red fluorescence is invisible to other animals, which allows these dragonfish extra light at dark ocean depths without attracting or signaling predators. Terrestrial biofluorescence Amphibians The Polka - dot tree frog, widely found in the Amazon was discovered to be the first fluorescent amphibian in 2017. The frog is pale green with dots in white, yellow or light red. The fluorescence of the frog was discovered unintentionally in Buenos Aires, Argentina. The fluorescence was traced to a new compound found in the lymph and skin glads. The main fluorescent compound is Hyloin - L1 and it gives a blue - green glow when exposed to violet or ultra violet light. Scientists behind the discovery say that the fluorescence can be used for communication. They also think that about 100 or 200 species of frogs are likely to be fluorescent. Butterflies Swallowtail ( Papilio ) butterflies have complex systems for emitting fluorescent light. Their wings contain pigment - infused crystals that provide directed fluorescent light. These crystals function to produce fluorescent light best when they absorb radiance from sky - blue light ( wavelength about 420 nm ). The wavelengths of light that the butterflies see the best correspond to the absorbance of the crystals in the butterfly 's wings. This likely functions to enhance the capacity for signaling. Parrots Parrots have fluorescent plumage that may be used in mate signaling. A study using mate - choice experiments on budgerigars ( Melopsittacus undulates ) found compelling support for fluorescent sexual signaling, with both males and females significantly preferring birds with the fluorescent experimental stimulus. This study suggests that the fluorescent plumage of parrots is not simply a by - product of pigmentation, but instead an adapted sexual signal. Considering the intricacies of the pathways that produce fluorescent pigments, there may be significant costs involved. Therefore, individuals exhibiting strong fluorescence may be honest indicators of high individual quality, since they can deal with the associated costs. Arachnids Fluorescing scorpion Spiders fluoresce under UV light and possess a huge diversity of fluorophores. Remarkably, spiders are the only known group in which fluorescence is `` taxonomically widespread, variably expressed, evolutionarily labile, and probably under selection and potentially of ecological importance for intraspecific and interspecific signaling. '' A study by Andrews et al. ( 2007 ) reveals that fluorescence has evolved multiple times across spider taxa, with novel fluorophores evolving during spider diversification. In some spiders, ultraviolet cues are important for predator - prey interactions, intraspecific communication, and camouflaging with matching fluorescent flowers. Differing ecological contexts could favor inhibition or enhancement of fluorescence expression, depending upon whether fluorescence helps spiders be cryptic or makes them more conspicuous to predators. Therefore, natural selection could be acting on expression of fluorescence across spider species. Scorpions also fluoresce. Plants The Mirabilis jalapa flower contains violet, fluorescent betacyanins and yellow, fluorescent betaxanthins. Under white light, parts of the flower containing only betaxanthins appear yellow, but in areas where both betaxanthins and betacyanins are present, the visible fluorescence of the flower is faded due to internal light - filtering mechanisms. Fluorescence was previously suggested to play a role in pollinator attraction, however, it was later found that the visual signal by fluorescence is negligible compared to the visual signal of light reflected by the flower. Chlorophyll fluoresces a weak red under ultraviolet light. Abiotic fluorescence Gemology, mineralogy and geology Fluorescence of Aragonite Gemstones, minerals, may have a distinctive fluorescence or may fluoresce differently under short - wave ultraviolet, long - wave ultraviolet, visible light, or X-rays. Many types of calcite and amber will fluoresce under shortwave UV, longwave UV and visible light. Rubies, emeralds, and diamonds exhibit red fluorescence under long - wave UV, blue and sometimes green light ; diamonds also emit light under X-ray radiation. Fluorescence in minerals is caused by a wide range of activators. In some cases, the concentration of the activator must be restricted to below a certain level, to prevent quenching of the fluorescent emission. Furthermore, the mineral must be free of impurities such as iron or copper, to prevent quenching of possible fluorescence. Divalent manganese, in concentrations of up to several percent, is responsible for the red or orange fluorescence of calcite, the green fluorescence of willemite, the yellow fluorescence of esperite, and the orange fluorescence of wollastonite and clinohedrite. Hexavalent uranium, in the form of the uranyl cation, fluoresces at all concentrations in a yellow green, and is the cause of fluorescence of minerals such as autunite or andersonite, and, at low concentration, is the cause of the fluorescence of such materials as some samples of hyalite opal. Trivalent chromium at low concentration is the source of the red fluorescence of ruby. Divalent europium is the source of the blue fluorescence, when seen in the mineral fluorite. Trivalent lanthanides such as terbium and dysprosium are the principal activators of the creamy yellow fluorescence exhibited by the yttrofluorite variety of the mineral fluorite, and contribute to the orange fluorescence of zircon. Powellite ( calcium molybdate ) and scheelite ( calcium tungstate ) fluoresce intrinsically in yellow and blue, respectively. When present together in solid solution, energy is transferred from the higher - energy tungsten to the lower - energy molybdenum, such that fairly low levels of molybdenum are sufficient to cause a yellow emission for scheelite, instead of blue. Low - iron sphalerite ( zinc sulfide ), fluoresces and phosphoresces in a range of colors, influenced by the presence of various trace impurities. Crude oil ( petroleum ) fluoresces in a range of colors, from dull - brown for heavy oils and tars through to bright - yellowish and bluish - white for very light oils and condensates. This phenomenon is used in oil exploration drilling to identify very small amounts of oil in drill cuttings and core samples. Organic liquids Organic solutions such anthracene or stilbene, dissolved in benzene or toluene, fluoresce with ultraviolet or gamma ray irradiation. The decay times of this fluorescence are on the order of nanoseconds, since the duration of the light depends on the lifetime of the excited states of the fluorescent material, in this case anthracene or stilbene. Scintillation is defined a flash of light produced in a transparent material by the passage of a particle ( an electron, an alpha particle, an ion, or a high - energy photon ). Stilbene and derivatives are used in scintillation counters to detect such particles. Stilbene is also one of the gain mediums used in dye lasers. Atmosphere Fluorescence is observed in the atmosphere when the air is under energetic electron bombardment. In cases such as the natural aurora, high - altitude nuclear explosions, and rocket - borne electron gun experiments, the molecules and ions formed have a fluorescent response to light. Common materials that fluoresce Vitamin B2 fluoresces yellow. Tonic water fluoresces blue due to the presence of quinine. Highlighter ink is often fluorescent due to the presence of pyranine. Banknotes, postage stamps and credit cards often have fluorescent security features. Applications of fluorescence Lighting Further information : Fluorescent lamp Fluorescent paint and plastic lit by UV tubes. Paintings by Beo Beyond The common fluorescent lamp relies on fluorescence. Inside the glass tube is a partial vacuum and a small amount of mercury. An electric discharge in the tube causes the mercury atoms to emit mostly ultraviolet light. The tube is lined with a coating of a fluorescent material, called the phosphor, which absorbs the ultraviolet and re-emits visible light. Fluorescent lighting is more energy - efficient than incandescent lighting elements. However, the uneven spectrum of traditional fluorescent lamps may cause certain colors to appear different than when illuminated by incandescent light or daylight. The mercury vapor emission spectrum is dominated by a short - wave UV line at 254 nm ( which provides most of the energy to the phosphors ), accompanied by visible light emission at 436 nm ( blue ), 546 nm ( green ) and 579 nm ( yellow - orange ). These three lines can be observed superimposed on the white continuum using a hand spectroscope, for light emitted by the usual white fluorescent tubes. These same visible lines, accompanied by the emission lines of trivalent europium and trivalent terbium, and further accompanied by the emission continuum of divalent europium in the blue region, comprise the more discontinuous light emission of the modern trichromatic phosphor systems used in many compact fluorescent lamp and traditional lamps where better color rendition is a goal. Fluorescent lights were first available to the public at the 1939 New York World 's Fair. Improvements since then have largely been better phosphors, longer life, and more consistent internal discharge, and easier - to - use shapes ( such as compact fluorescent lamps ). Some high - intensity discharge ( HID ) lamps couple their even - greater electrical efficiency with phosphor enhancement for better color rendition. White light - emitting diodes ( LEDs ) became available in the mid-1990s as LED lamps, in which blue light emitted from the semiconductor strikes phosphors deposited on the tiny chip. The combination of the blue light that continues through the phosphor and the green to red fluorescence from the phosphors produces a net emission of white light. Glow sticks sometimes utilize fluorescent materials to absorb light from the chemiluminescent reaction and emit light of a different color. Analytical chemistry Many analytical procedures involve the use of a fluorometer, usually with a single exciting wavelength and single detection wavelength. Because of the sensitivity that the method affords, fluorescent molecule concentrations as low as 1 part per trillion can be measured. Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow. Also, TLC plates can be visualized if the compounds or a coloring reagent is fluorescent. Fluorescence is most effective when there is a larger ratio of atoms at lower energy levels in a Boltzmann distribution. There is, then, a higher probability of excitement and release of photons by lower - energy atoms, making analysis more efficient. Spectroscopy Main article : Fluorescence spectroscopy Usually the setup of a fluorescence assay involves a light source, which may emit many different wavelengths of light. In general, a single wavelength is required for proper analysis, so, in order to selectively filter the light, it is passed through an excitation monochromator, and then that chosen wavelength is passed through the sample cell. After absorption and re-emission of the energy, many wavelengths may emerge due to Stokes shift and various electron transitions. To separate and analyze them, the fluorescent radiation is passed through an emission monochromator, and observed selectively by a detector. Biochemistry and medicine Main article : Fluorescence in the life sciences Endothelial cells under the microscope with three separate channels marking specific cellular components Fluorescence in the life sciences is used generally as a non-destructive way of tracking or analysis of biological molecules by means of the fluorescent emission at a specific frequency where there is no background from the excitation light, as relatively few cellular components are naturally fluorescent ( called intrinsic or autofluorescence ). In fact, a protein or other component can be `` labelled '' with an extrinsic fluorophore, a fluorescent dye that can be a small molecule, protein, or quantum dot, finding a large use in many biological applications. The quantification of a dye is done with a spectrofluorometer and finds additional applications in : Microscopy When scanning the fluorescence intensity across a plane one has fluorescence microscopy of tissues, cells, or subcellular structures, which is accomplished by labeling an antibody with a fluorophore and allowing the antibody to find its target antigen within the sample. Labelling multiple antibodies with different fluorophores allows visualization of multiple targets within a single image ( multiple channels ). DNA microarrays are a variant of this. Immunology : An antibody is first prepared by having a fluorescent chemical group attached, and the sites ( e.g., on a microscopic specimen ) where the antibody has bound can be seen, and even quantified, by the fluorescence. FLIM ( Fluorescence Lifetime Imaging Microscopy ) can be used to detect certain bio-molecular interactions that manifest themselves by influencing fluorescence lifetimes. Cell and molecular biology : detection of colocalization using fluorescence - labelled antibodies for selective detection of the antigens of interest using specialized software, such as CoLocalizer Pro. Other techniques FRET ( Förster resonance energy transfer, also known as fluorescence resonance energy transfer ) is used to study protein interactions, detect specific nucleic acid sequences and used as biosensors, while fluorescence lifetime ( FLIM ) can give an additional layer of information. Biotechnology : biosensors using fluorescence are being studied as possible Fluorescent glucose biosensors. Automated sequencing of DNA by the chain termination method ; each of four different chain terminating bases has its own specific fluorescent tag. As the labelled DNA molecules are separated, the fluorescent label is excited by a UV source, and the identity of the base terminating the molecule is identified by the wavelength of the emitted light. FACS ( fluorescence - activated cell sorting ). One of several important cell sorting techniques used in the separation of different cell lines ( especially those isolated from animal tissues ). DNA detection : the compound ethidium bromide, in aqueous solution, has very little fluorescence, as it is quenched by water. Ethidium bromide 's fluorescence is greatly enhanced after it binds to DNA, so this compound is very useful in visualising the location of DNA fragments in agarose gel electrophoresis. Intercalated ethidium is in a hydrophobic environment when it is between the base pairs of the DNA, protected from quenching by water which is excluded from the local environment of the intercalated ethidium. Ethidium bromide may be carcinogenic -- an arguably safer alternative is the dye SYBR Green. FIGS ( Fluorescence image - guided surgery ) is a medical imaging technique that uses fluorescence to detect properly labeled structures during surgery. Intravascular fluorescence is a catheter - based medical imaging technique that uses fluorescence to detect high - risk features of atherosclerosis and unhealed vascular stent devices. Plaque autofluorescence has been used in a first - in - man study in coronary arteries in combination with optical coherence tomography. Molecular agents has been also used to detect specific features, such as stent fibrin accumulation and enzymatic activity related to artery inflammation. SAFI ( species altered fluorescence imaging ) an imaging technique in electrokinetics and microfluidics. It uses non-electromigrating dyes whose fluorescence is easily quenched by migrating chemical species of interest. The dye ( s ) are usually seeded everywhere in the flow and differential quenching of their fluorescence by analytes is directly observed. Fluorescence - based assays for screening toxic chemicals. The optical assays consist of a mixture of environmental - sensitive fluorescent dyes and human skin cells that generate fluorescence spectra patterns. This approach can reduce the need for laboratory animals in biomedical research and pharmaceutical industry. Forensics Fingerprints can be visualized with fluorescent compounds such as ninhydrin or DFO ( 1, 8 - Diazafluoren - 9 - one ). Blood and other substances are sometimes detected by fluorescent reagents, like fluorescein. Fibers, and other materials that may be encountered in forensics or with a relationship to various collectibles, are sometimes fluorescent. Mechanical engineering Fluorescent penetrant inspection is used to find cracks and other defects on the surface of a part. Dye tracing, using fluorescent dyes, is used to find leaks in liquid and gas plumbing systems. Signage Fluorescent colors are frequently used in signage, particularly road signs. Fluorescent colors are generally recognizable at longer ranges than their non-fluorescent counterparts, with fluorescent orange being particularly noticeable. This property has led to its frequent use in safety signs and labels. Optical brighteners Main article : Optical brightener Fluorescent compounds are often used to enhance the appearance of fabric and paper, causing a `` whitening '' effect. A white surface treated with an optical brightener can emit more visible light than that which shines on it, making it appear brighter. The blue light emitted by the brightener compensates for the diminishing blue of the treated material and changes the hue away from yellow or brown and toward white. Optical brighteners are used in laundry detergents, high brightness paper, cosmetics, high - visibility clothing and more. See also Absorption - re-emission atomic line filters use the phenomenon of fluorescence to filter light extremely effectively. Black light Blacklight paint Fluorescence correlation spectroscopy Fluorescence image - guided surgery Fluorescence in plants Fluorescence spectroscopy Fluorescent lamp Fluorescent multilayer card Fluorescent Multilayer Disc Fluorometer High - visibility clothing Integrated fluorometer Laser - induced fluorescence List of light sources Microbial art, using fluorescent bacteria Mössbauer effect, resonant fluorescence of gamma rays Organic light - emitting diodes can be fluorescent Phosphorescence Phosphor thermometry, the use of phosphorescence to measure temperature. Spectroscopy Two - photon absorption Vibronic spectroscopy X-ray fluorescence References ^ Jump up to : Acuña, A. Ulises ; Amat - Guerri, Francisco ; Morcillo, Purificación ; Liras, Marta ; Rodríguez, Benjamín ( 2009 ). `` Structure and Formation of the Fluorescent Compound of Lignum nephriticum '' ( PDF ). Organic Letters. 11 ( 14 ) : 3020 -- 3023. doi : 10.1021 / ol901022g. PMID 19586062. Archived ( PDF ) from the original on 28 July 2013. Jump up ^ Safford, William Edwin ( 1916 ). `` Lignum nephriticum ''. Annual report of the Board of Regents of the Smithsonian Institution ( PDF ). Washington : Government Printing Office. pp. 271 -- 298. Jump up ^ Valeur, B. ; Berberan - Santos, M.R.N. ( 2011 ). `` A Brief History of Fluorescence and Phosphorescence before the Emergence of Quantum Theory ''. Journal of Chemical Education. 88 ( 6 ) : 731 -- 738. Bibcode : 2011JChEd... 88... 731V. doi : 10.1021 / ed100182h. 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( Paris, France : Bachelier and Huzard, 1822 ), vol. 1, p. 512 Archived 17 January 2017 at the Wayback Machine... Fluorite is called `` chaux fluatée '' by Haüy : ``... violette par réflection, et verdâtre par transparence au Derbyshire. '' ( ( the color of fluorite is ) violet by reflection, and greenish by transmission in ( specimens from ) Derbyshire. ) Jump up ^ Brewster, David ( 1834 ). `` On the colours of natural bodies ''. Transactions of the Royal Society of Edinburgh. 12 ( 2 ) : 538 -- 545. doi : 10.1017 / s0080456800031203. Archived from the original on 17 January 2017. On page 542, Brewster mentions that when white light passes through an alcoholic solution of chlorophyll, red light is reflected from it. Jump up ^ Herschel, John ( 1845 ). `` On a case of superficial colour presented by a homogeneous liquid internally colourless ''. Philosophical Transactions of the Royal Society of London. 135 : 143 -- 145. doi : 10.1098 / rstl. 1845.0004. 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Jump up ^ Moczko, E ; Mirkes, EM ; Cáceres, C ; Gorban, AN ; Piletsky, S ( 2016 ). `` Fluorescence - based assay as a new screening tool for toxic chemicals ''. Scientific Reports. 6 : 33922. Bibcode : 2016NatSR... 633922M. doi : 10.1038 / srep33922. PMC 5031998. PMID 27653274. Jump up ^ Hawkins, H. Gene ; Carlson, Paul John and Elmquist, Michael ( 2000 ) `` Evaluation of fluorescent orange signs '' Archived 4 March 2016 at the Wayback Machine., Texas Transportation Institute Report 2962 - S. Bibliography Lakowicz, Joseph R. ( 1999 ). Principles of Fluorescence Spectroscopy. Kluwer Academic / Plenum Publishers. ISBN 978 - 0 - 387 - 31278 - 1. External links Wikimedia Commons has media related to Fluorescence. Fluorophores.org, the database of fluorescent dyes FSU.edu, Basic Concepts in Fluorescence `` A nano - history of fluorescence '' lecture by David Jameson Excitation and emission spectra of various fluorescent dyes Database of fluorescent minerals with pictures, activators and spectra ( fluomin.org ) `` Biofluorescent Night Dive -- Dahab / Red Sea ( Egypt ), Masbat Bay / Mashraba, `` Roman Rock '' ``. YouTube. 9 October 2012. Steffen O. Beyer. `` FluoPedia.org : Publications ''. fluopedia.org. Steffen O. Beyer. `` FluoMedia.org : Science ''. fluomedia.org. Lamps Lighting Concepts Accent lighting Color temperature Efficiency Glare Lamp Light fixture Light pollution Lightbulb socket Task lighting Methods of generation Incandescent Regular Halogen Nernst Parabolic aluminized reflector ( PAR ) Luminescent Fluorescent Fluorescent lamp ( compact ) Fluorescent induction Photoluminescent laser lamp Chemiluminescent Solid - state LED bulb Cathodoluminescent Electron - stimulated Electroluminescent field - induced polymer Combustion Acetylene / Carbide Argand Campfire Candle Carcel Diya Flare Gas Kerosene Lantern Limelight Oil Rushlight Safety Tilley Torch Electric arc Carbon arc Klieg light Yablochkov candle Gas discharge Deuterium arc Neon Plasma Sulfur Xenon arc Xenon flash High - intensity discharge ( HID ) Hydrargyrum medium - arc iodide ( HMI ) Hydrargyrum quartz iodide ( HQI ) Mercury - vapor Metal - halide ceramic Sodium vapor Theatrical Cinematic Floodlight Footlight Gobo Scoop Spotlight ellipsoidal reflector Stage lighting instrument Stationary Aircraft warning Balanced - arm lamp Chandelier Emergency light Gas lighting Gooseneck lamp Intelligent street lighting Light tube Nightlight Neon lighting Pendant light Recessed light Sconce Street light Torchère Track lighting Troffer Mobile Flashlight tactical Glow stick Headlamp ( outdoor ) Lantern Laser pointer Navigation light Searchlight Solar lamp Industrial Scientific Germicidal Grow light Infrared lamp Stroboscope Tanning Display Decorative Aroma lamp Black light Bubble light Christmas lights Crackle tube DJ lighting Electroluminescent wire Lava lamp Marquee Plasma globe Strobe light Related topics Bioluminescence Chemiluminescence Electroluminescence Laser Photoluminescence Radioluminescence GND : 4154818 - 8 NDL : 00565352 Retrieved from `` https://en.wikipedia.org/w/index.php?title=Fluorescence&oldid=827795253 '' Categories : Fluorescence Dyes Molecular biology Radiochemistry Hidden categories : Webarchive template wayback links All articles with dead external links Articles with dead external links from January 2018 Articles with permanently dead external links Use dmy dates from July 2012 All articles with unsourced statements Articles with unsourced statements from August 2011 Articles with unsourced statements from September 2015 Articles with unsourced statements from March 2011 Articles with unsourced statements from July 2010 Pages using div col with deprecated parameters Wikipedia articles with GND identifiers Talk Contents About Wikipedia Asturianu বাংলা Български Bosanski Català Čeština Dansk Deutsch Eesti Ελληνικά Español Euskara فارسی Français Gaeilge Galego 한국어 Հայերեն हिन्दी Hrvatski Bahasa Indonesia Italiano עברית Қазақша Latina Lietuvių Magyar Nederlands 日本 語 Norsk Norsk nynorsk Occitan Piemontèis Polski Português Română Русский Simple English Slovenčina Српски / srpski Srpskohrvatski / српскохрватски Suomi Svenska தமிழ் Türkçe Українська اردو Tiếng Việt 粵語 中文 Edit links This page was last edited on 26 February 2018, at 19 : 52. 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Question: what is something that gives out light called
Answer: fluorescent