Translation ( biology ) - wikipedia Translation ( biology ) Overview of the translation of eukaryotic messenger RNA Diagram showing the translation of mRNA and the synthesis of proteins by a ribosome In molecular biology and genetics, translation is the process in which ribosomes in the cytoplasm or ER synthesize proteins after the process transcription of DNA to RNA in the cell 's nucleus. The entire process is called gene expression. In translation, messenger RNA ( mRNA ) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide. The polypeptide later folds into an active protein and performs its functions in the cell. The ribosome facilitates decoding by inducing the binding of complementary tRNA anticodon sequences to mRNA codons. The tRNAs carry specific amino acids that are chained together into a polypeptide as the mRNA passes through and is `` read '' by the ribosome. Translation proceeds in three phases : Initiation : The ribosome assembles around the target mRNA. The first tRNA is attached at the start codon. Elongation : The tRNA transfers an amino acid to the tRNA corresponding to the next codon. The ribosome then moves ( translocates ) to the next mRNA codon to continue the process, creating an amino acid chain. Termination : When a stop codon is reached, the ribosome releases the polypeptide. In prokaryotes ( bacteria ), translation occurs in the cytoplasm, where the large and small subunits of the ribosome bind to the mRNA. In eukaryotes, translation occurs in the cytosol or across the membrane of the endoplasmic reticulum in process called co-tanslational translocation. In co-translational translocation, the entire ribosome / mRNA complex binds to the outer membrane of the rough endoplasmic reticulum ( ER ) and the new protein is synthesized and released into the ER ; the newly created polypeptide can be stored inside the ER for future vesicle transport and secretion outside the cell, or immediately secreted. Many types of transcribed RNA, such as transfer RNA, ribosomal RNA, and small nuclear RNA, do not undergo translation into proteins. A number of antibiotics act by inhibiting translation. These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin. Prokaryotic ribosomes have a different structure from that of eukaryotic ribosomes, and thus antibiotics can specifically target bacterial infections without any harm to a eukaryotic host 's cells. Contents 1 Basic mechanisms 2 Genetic code 2.1 Translation tables 3 See also 4 References 5 Further reading 6 External links Basic mechanisms Further information : Prokaryotic translation and Eukaryotic translation A ribosome translating a protein that is secreted into the endoplasmic reticulum. tRNAs are colored dark blue. Tertiary structure of tRNA. CCA tail in orange, Acceptor stem in purple, D arm in red, Anticodon arm in blue with Anticodon in black, T arm in green. The basic process of protein production is addition of one amino acid at a time to the end of a protein. This operation is performed by a ribosome. A ribosome is made up of two subunits, a small subunit and a large subunit. these subunits come together before translation of mRNA into a protein to provide a location for translation to be carried out and a polypeptide to be produced. The choice of amino acid type to add is determined by an mRNA molecule. Each amino acid added is matched to a three nucleotide subsequence of the mRNA. For each such triplet possible, the corresponding amino acid is accepted. The successive amino acids added to the chain are matched to successive nucleotide triplets in the mRNA. In this way the sequence of nucleotides in the template mRNA chain determines the sequence of amino acids in the generated amino acid chain. Addition of an amino acid occurs at the C - terminus of the peptide and thus translation is said to be amino - to - carboxyl directed. The mRNA carries genetic information encoded as a ribonucleotide sequence from the chromosomes to the ribosomes. The ribonucleotides are `` read '' by translational machinery in a sequence of nucleotide triplets called codons. Each of those triplets codes for a specific amino acid. The ribosome molecules translate this code to a specific sequence of amino acids. The ribosome is a multisubunit structure containing rRNA and proteins. It is the `` factory '' where amino acids are assembled into proteins. tRNAs are small noncoding RNA chains ( 74 - 93 nucleotides ) that transport amino acids to the ribosome. tRNAs have a site for amino acid attachment, and a site called an anticodon. The anticodon is an RNA triplet complementary to the mRNA triplet that codes for their cargo amino acid. Aminoacyl tRNA synthetases ( enzymes ) catalyze the bonding between specific tRNAs and the amino acids that their anticodon sequences call for. The product of this reaction is an aminoacyl - tRNA. This aminoacyl - tRNA is carried to the ribosome by EF - Tu, where mRNA codons are matched through complementary base pairing to specific tRNA anticodons. Aminoacyl - tRNA synthetases that mispair tRNAs with the wrong amino acids can produce mischarged aminoacyl - tRNAs, which can result in inappropriate amino acids at the respective position in protein. This `` mistranslation '' of the genetic code naturally occurs at low levels in most organisms, but certain cellular environments cause an increase in permissive mRNA decoding, sometimes to the benefit of the cell. The ribosome has three sites for tRNA to bind. They are the aminoacyl site ( abbreviated A ), the peptidyl site ( abbreviated P ) and the exit site ( abbreviated E ). With respect to the mRNA, the three sites are oriented 5 ' to 3 ' E-P-A, because ribosomes move toward the 3 ' end of mRNA. The A-site binds the incoming tRNA with the complementary codon on the mRNA. The P - site holds the tRNA with the growing polypeptide chain. The E-site holds the tRNA without its amino acid. When an aminoacyl - tRNA initially binds to its corresponding codon on the mRNA, it is in the A site. Then, a peptide bond forms between the amino acid of the tRNA in the A site and the amino acid of the charged tRNA in the P site. The growing polypeptide chain is transferred to the tRNA in the A site. Translocation occurs, moving the tRNA in the P site, now without an amino acid, to the E site ; the tRNA that was in the A site, now charged with the polypeptide chain, is moved to the P site. The tRNA in the E site leaves and another aminoacyl - tRNA enters the A site to repeat the process. After the new amino acid is added to the chain, and after the mRNA is released out of the nucleus and into the ribosome 's core, the energy provided by the hydrolysis of a GTP bound to the translocase EF - G ( in prokaryotes ) and eEF - 2 ( in eukaryotes ) moves the ribosome down one codon towards the 3 ' end. The energy required for translation of proteins is significant. For a protein containing n amino acids, the number of high - energy phosphate bonds required to translate it is 4n - 1. The rate of translation varies ; it is significantly higher in prokaryotic cells ( up to 17 - 21 amino acid residues per second ) than in eukaryotic cells ( up to 6 - 9 amino acid residues per second ). Even though the ribosomes are usually considered accurate and processive machines, the translation process is subject to errors that can lead either to the synthesis of erroneous proteins or to the premature abandonment of translation. The rate of error in synthesizing proteins has been estimated to be between 1 / 10 and 1 / 10 misincorporated amino acids, depending on the experimental conditions. The rate of premature translation abandonment, instead, has been estimated to be of the order of magnitude of 10 events per translated codon. The correct amino acid is covalently bonded to the correct transfer RNA ( tRNA ) by amino acyl transferases. The amino acid is joined by its carboxyl group to the 3 ' OH of the tRNA by an ester bond. When the tRNA has an amino acid linked to it, the tRNA is termed `` charged ''. Initiation involves the small subunit of the ribosome binding to the 5 ' end of mRNA with the help of initiation factors ( IF ). Termination of the polypeptide happens when the A site of the ribosome faces a stop codon ( UAA, UAG, or UGA ) on the mRNA. tRNA usually can not recognize or bind to stop codons. Instead, the stop codon induces the binding of a release factor protein that prompts the disassembly of the entire ribosome / mRNA complex and the hydrolysis and the release of the polypeptide chain from the ribosome. Drugs or special sequence motifs on the mRNA can change the ribosomal structure so that near - cognate tRNAs are bound to the stop codon instead of the release factors. In such cases of ' translational readthrough ', translation continues until the ribosome encounters the next stop codon. The process of translation is highly regulated in both eukaryotic and prokaryotic organisms. Regulation of translation can impact the global rate of protein synthesis which is closely coupled to the metabolic and proliferative state of a cell. In addition, recent work has revealed that genetic differences and their subsequent expression as mRNAs can also impact translation rate in an RNA - specific manner. Genetic Code Main article : Genetic code Whereas other aspects such as the 3D structure, called tertiary structure, of protein can only be predicted using sophisticated algorithms, the amino acid sequence, called primary structure, can be determined solely from the nucleic acid sequence with the aid of a translation table. This approach may not give the correct amino acid composition of the protein, in particular if unconventional amino acids such as selenocysteine are incorporated into the protein, which is coded for by a conventional stop codon in combination with a downstream hairpin ( SElenoCysteine Insertion Sequence, or SECIS ). There are many computer programs capable of translating a DNA / RNA sequence into a protein sequence. Normally this is performed using the Standard Genetic Code, however, few programs can handle all the `` special '' cases, such as the use of the alternative initiation codons. For instance, the rare alternative start codon CTG codes for Methionine when used as a start codon, and for Leucine in all other positions. Example : Condensed translation table for the Standard Genetic Code ( from the NCBI Taxonomy webpage ). AAs = FFLLSSSSYY * * CC * WLLLLPPPPHHQQRRRRIIIMTTTTNNKKSSRRVVVVAAAADDEEGGGG Starts = -- - M -- -- -- -- -- -- -- - M -- -- -- -- -- -- -- - M -- -- -- -- -- -- -- -- -- -- -- -- -- -- Base1 = TTTTTTTTTTTTTTTTCCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAGGGGGGGGGGGGGGGG Base2 = TTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGGTTTTCCCCAAAAGGGG Base3 = TCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAGTCAG Translation tables Even when working with ordinary eukaryotic sequences such as the Yeast genome, it is often desired to be able to use alternative translation tables -- namely for translation of the mitochondrial genes. Currently the following translation tables are defined by the NCBI Taxonomy Group for the translation of the sequences in GenBank : The Standard The Vertebrate Mitochondrial Code The Yeast Mitochondrial Code The Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma / Spiroplasma Code The Invertebrate Mitochondrial Code The Ciliate, Dasycladacean and Hexamita Nuclear Code The Echinoderm and Flatworm Mitochondrial Code The Euplotid Nuclear Code The Bacterial and Plant Plastid Code The Alternative Yeast Nuclear Code The Ascidian Mitochondrial Code The Alternative Flatworm Mitochondrial Code Blepharisma Nuclear Code Chlorophycean Mitochondrial Code Trematode Mitochondrial Code Scenedesmus obliquus mitochondrial Code Thraustochytrium Mitochondrial Code See also DNA codon table Expanded genetic code Protein methods Start codon References Jump up ^ Biology. McGraw hill education. p. 249. ISBN 978 - 981 - 4581 - 85 - 1. Jump up ^ Neill C ( 1996 ). Biology ( Fourth ed. ). The Benjamin / Cummings Publishing Company. pp. 309 -- 310. ISBN 0 - 8053 - 1940 - 9. Jump up ^ Stryer L ( 2002 ). Biochemistry ( Fifth ed. ). W.H. Freeman and Company. p. 826. ISBN 0 - 7167 - 4684 - 0. Jump up ^ Moghal A, Mohler K, Ibba M ( November 2014 ). `` Mistranslation of the genetic code ''. FEBS Letters. 588 ( 23 ) : 4305 -- 10. doi : 10.1016 / j. febslet. 2014.08. 035. PMC 4254111. PMID 25220850. Jump up ^ Griffiths A ( 2008 ). `` 9 ''. Introduction to Genetic Analysis ( 9th ed. ). New York : W.H. Freeman and Company. pp. 335 -- 339. ISBN 978 - 0 - 7167 - 6887 - 6. Jump up ^ Ross JF, Orlowski M ( February 1982 ). `` Growth - rate - dependent adjustment of ribosome function in chemostat - grown cells of the fungus Mucor racemosus ''. Journal of Bacteriology. 149 ( 2 ) : 650 -- 3. PMC 216554. PMID 6799491. Jump up ^ Wohlgemuth, I ; Pohl, C ; Mittelstaet, J ; Konevega, AL ; Rodnina, MV ( 27 October 2011 ). `` Evolutionary optimization of speed and accuracy of decoding on the ribosome ''. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 366 ( 1580 ) : 2979 -- 86. doi : 10.1098 / rstb. 2011.0138. PMC 3158919. PMID 21930591. Jump up ^ Sin, C ; Valleriani, A ; Chiarugi, D ( 1 March 2016 ). `` Quantitative assessment of ribosome drop - off in E. coli ''. Nucleic Acids Research. 44 ( 6 ) : 2528. doi : 10.1093 / nar / gkw137. PMC 4824120. PMID 26935582. Jump up ^ Schueren F and Thoms S ( 2016 ). `` Functional Translational Readthrough : A Systems Biology Perspective ''. PLOS Genetics. 12 ( e1006196 ) : 12. doi : 10.1371 / JOURNAL. PGEN. 1006196. PMC 4973966. PMID 27490485. Jump up ^ Cenik C, Cenik ES, Byeon GW, Grubert F, Candille SI, Spacek D, Alsallakh B, Tilgner H, Araya CL, Tang H, Ricci E, Snyder MP ( November 2015 ). `` Integrative analysis of RNA, translation, and protein levels reveals distinct regulatory variation across humans ''. Genome Research. 25 ( 11 ) : 1610 -- 21. doi : 10.1101 / gr. 193342.115. PMC 4617958. PMID 26297486. Further reading Champe PC, Harvey RA, Ferrier DR ( 2004 ). Lippincott 's Illustrated Reviews : Biochemistry ( 3rd ed. ). Hagerstwon, MD : Lippincott Williams & Wilkins. ISBN 0 - 7817 - 2265 - 9. Cox M, Nelson DR, Lehninger AL ( 2005 ). Lehninger principles of biochemistry ( 4th ed. ). San Francisco... : W.H. Freeman. ISBN 0 - 7167 - 4339 - 6. Malys N, McCarthy JE ( March 2011 ). `` Translation initiation : variations in the mechanism can be anticipated ''. Cellular and Molecular Life Sciences. 68 ( 6 ) : 991 -- 1003. doi : 10.1007 / s00018 - 010 - 0588 - z. PMID 21076851. External links Wikimedia Commons has media related to Translation ( biology ). Virtual Cell Animation Collection : Introducing Translation Translate tool ( from DNA or RNA sequence ) Gene expression Introduction to genetics Genetic code Central dogma DNA → RNA → Protein Special transfers RNA → RNA RNA → DNA Protein → Protein Transcription Types Bacterial Eukaryotic Key elements Transcription factor RNA polymerase Promoter Post-transcription Precursor mRNA ( pre-mRNA / hnRNA ) 5 ' capping Splicing Polyadenylation Histone acetylation and deacetylation Translation Types Prokaryotic Eukaryotic Key elements Ribosome Transfer RNA ( tRNA ) Ribosome - nascent chain complex ( RNC ) Post-translational modification ( functional groups peptides structural changes ) Regulation Epigenetic imprinting Transcriptional Gene regulatory network cis - regulatory element lac operon Post-transcriptional sequestration ( P - bodies ) alternative splicing microRNA Translational Post-translational reversible irreversible Influential people François Jacob Jacques Monod Protein biosynthesis : translation ( prokaryotic, eukaryotic ) Proteins Initiation factor Prokaryotic PIF - 1 PIF - 2 PIF - 3 Archaeal aIF1 aIF2 aIF5 aIF6 Eukaryotic eIF1 eIF1 EIF1AX AY 1B eIF2 EIF2S1 EIF2S2 EIF2S3 EIF2B1 EIF2B2 EIF2B3 EIF2B4 EIF2B5 EIF - 2 kinase eIF2A eIF2D eIF3 EIF3A eIF4 EIF4A2 A3 E1 E2 E3 G1 G2 G3 eIF5 EIF5 EIF5A A2 5B eIF6 EIF6 Elongation factor Prokaryotic EF - Tu EF - Ts EF - G Archaeal aEF - 1 aEF - 2 Eukaryotic EEF - 1 EEF1A1 EEF1A2 EEF1A3 EEF1B1 EEF1B2 EEF1B3 EEF1B4 EEF1D EEF1E1 EEF1G EEF2 Release factor Prokaryotic Archaeal Eukaryotic ( ETF1 ) Ribosomal Proteins Cytoplasmic 60S subunit RPL3 RPL4 RPL5 RPL6 RPL7 RPL7A RPL8 RPL9 RPL10 RPL10A RPL10 - like RPL11 RPL12 RPL13 RPL13A RPL14 RPL15 RPL16 RPL17 RPL18 RPL18A RPL19 RPL20 RPL21 RPL22 RPL23 RPL23A RPL24 RPL25 RPL26 RPL27 RPL27A RPL28 RPL29 RPL30 RPL31 RPL32 RPL33 RPL34 RPL35 RPL35A RPL36 RPL36A RPL37 RPL37A RPL38 RPL39 RPL40 RPL41 RPLP0 RPLP1 RPLP2 RPLP3 RRP15 - like RSL24D1 40S subunit RPSA RPS2 RPS3 RPS3A RPS4 ( RPS4X, RPS4Y1, RPS4Y2 ) RPS5 RPS6 RPS7 RPS8 RPS9 RPS10 RPS11 RPS12 RPS13 RPS14 RPS15 RPS15A RPS16 RPS17 RPS18 RPS19 RPS20 RPS21 RPS22 RPS23 RPS24 RPS25 RPS26 RPS27 RPS27A RPS28 RPS29 RPS30 RACK1 Mitochondrial 39S subunit MRPL1 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 28S subunit MRPS1 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Other concepts Aminoacyl tRNA synthetase Reading frame Start codon Stop codon Shine - Dalgarno sequence / Kozak consensus sequence Retrieved from `` https://en.wikipedia.org/w/index.php?title=Translation_(biology)&oldid=815577147 '' Categories : Molecular biology Protein biosynthesis Gene expression Hidden categories : All articles with unsourced statements Articles with unsourced statements from April 2009 Talk Contents About Wikipedia Azərbaycanca বাংলা Bân - lâm - gú Беларуская Български Bosanski Català Čeština Dansk Deutsch Eesti Ελληνικά Español Euskara فارسی Français Gaeilge Galego 한국어 Հայերեն Hrvatski Bahasa Indonesia Italiano עברית ქართული Қазақша Latina Lietuvių Magyar Македонски Nederlands 日本 語 Norsk nynorsk Occitan Polski Português Русский Scots Simple English Slovenčina Slovenščina کوردی Српски / srpski Srpskohrvatski / српскохрватски Suomi Svenska தமிழ் ไทย Türkçe Українська اردو 中文 Edit links This page was last edited on 15 December 2017, at 18 : 06. 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Question: where is mrna translated into an amino acid sequence
Answer: in a ribosome, outside the nucleus