P - site - wikipedia P - site The P - site ( for peptidyl ) is the second binding site for tRNA in the ribosome. The other two sites are the A-site ( aminoacyl ), which is the first binding site in the ribosome, and the E-site ( exit ), is the third and final binding site in the ribosome. During Protein translation, the P - site holds the tRNA which is linked to the growing polypeptide chain. When a stop codon is reached, the peptidyl - tRNA bond of the tRNA located in the P - site is cleaved releasing the newly synthesized protein. During the translocation step of the elongation phase, the mRNA is advanced by one codon, coupled to movement of the tRNAs from the ribosomal A to P and P to E sites, catalyzed by elongation factor EF - G. Contents 1 Overview 2 Structure 3 tRNA targeting antibiotics 4 References Overview The ribosomal P - site plays a vital role in all phases of translation. Initiation involves recognition of the start codon ( AUG ) by initiator tRNA in the P - site, elongation phase involves passage of many elongator tRNAs through the P site, termination phase involves hydrolysis of the mature polypeptide from tRNA bound to the P - site, and ribosome recycling involves release of deacylated tRNA. Binding a tRNA to the P - site in the presence of mRNA establishes codon - anticodon interaction and this interaction is important for small subunit ribosome ( 30S ) contacts to the tRNA. The classical two - state model proposes that ribosome contains two binding sites for tRNA, P - site and A-site. The A-site binds to incoming to aminoacyl - tRNA which has the anti-codon for the corresponding codon in the mRNA presented in the A-site. After peptide formation between the C - terminal carbonyl group of the growing polypeptide chain ( attached to a P - site bound tRNA ) and the amino group of the aminoacyl - tRNA ( A-site bound ), the polypeptide chain is then attached to the tRNA in the A-site. The deacylated tRNA remains in the P - site and gets released once the peptidyl - tRNA is transferred to the P - site. Chemical modification experiments provided evidence of a hybrid model, where tRNAs can sample a hybrid state of binding during elongation phase ( pre-translocation step ). These hybrid states of binding are in which acceptor and anti-codon ends of tRNA are in different sites ( A, P and E ). Using Chemical probing methods, a set of phylogenetically conserved bases in ribosomal RNA where the tRNA binds has been examined and suggested to be directly involved in the binding of tRNA to the prokaryotic ribosome. Correlation of such site specific protected bases in rRNA and occupancy of the A, P and E sites has allowed diagnostic assays of these bases to study the location of tRNA in any given state of the translational cycle. Authors proposed a hybrid model where higher affinity of the deactivated tRNA and peptide tRNA for the E and P sites of the 50S subunit, thermodynamically favour P / P to P / E and A / A to A / P transitions, which were further demonstrated through cryo - EM experiments. Also, single molecule FRET studies have detected fluctuations in the positions of tRNAs, leading to the conclusion that the classical ( A / A-P / P ) and hybrid states ( A / P-P / E ) of the tRNAs are certainly in dynamic equilibrium. Prior to peptide bond formation, an aminoacyl - tRNA is bound in the A-site, a peptidyl - tRNA is bound in the P - site, and a deacylated tRNA ( ready to exit from the ribosome ) is bound to the E-site. Translation moves the tRNA from the A-site through the P - and E-sites, with the exception of the initiator tRNA, which binds directly to the P - site. Recent experiments have reported that protein translation can also initiate from A-site. Using Toeprinting assay, it has been shown that Protein Synthesis initiates from the A-site of the Ribosome ( Eukaryotic ) in the cricket paralysis virus ( CrPV ). IGR - IRES ( Intragenic regions - internal ribosome entry sites ) can assemble 80S ribosomes from 40S and 60S ribosomal subunits in the absence of eIF2, Met - tRNAi, or GTP hydrolysis and without a coding triplet in the ribosomal P - site. Authors also showed IGR - IRES can direct translation of a protein whose N - terminal residue is not methionine. Structure The complete three dimensional structure of the T. thermophilus 70S ribosome was determined using X-ray crystallography, containing mRNA and tRNAs bound to the P and E sites at 5.5 Å resolution and to the A site at 7 Å resolution. Authors found that all three tRNA binding sites ( A, P, and E ) of the ribosome contact all three respective tRNAs at universally conserved parts of their structures ; this allows the ribosome to bind different tRNA species in precisely the same way. The translocation step of protein synthesis inescapably requires movements of 20 Å or more by the tRNAs, as they move from the A to P to E sites TRNA targeting Antibiotics Oxazolidines ( e.g. linezolid ) prevent the binding of the initiator tRNA at the P - site. Oxazolidines have been demonstrated to pleiotropically affect initiator - tRNA binding, EF - P ( elongation factor P ) stimulated synthesis of peptide bonds, and EF - G - mediated translocation of initiator - tRNA into the P - site. Macrolide, Lincosamide and Streptogramin class of antibiotics prevent peptide bond formation and / or the translocation of tRNA from the A-site to the P - site on the ribosome that eventually leads to interference with the elongation step and thus the inhibition of protein translation. References Jump up ^ Lodish, Harvey ( 2013 ). Molecular cell biology ( Seventh ed. ). New York : Worth Publ. pp. 141 -- 143. ISBN 978 - 1429234139. Jump up ^ Rodnina, MV ; Savelsbergh, A ; Katunin, VI ; Wintermeyer, W ( 2 January 1997 ). `` Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome ''. Nature. 385 ( 6611 ) : 37 -- 41. doi : 10.1038 / 385037a0. PMID 8985244. Jump up ^ Schäfer, MA ; Tastan, AO ; Patzke, S ; Blaha, G ; Spahn, CM ; Wilson, DN ; Nierhaus, KH ( 24 May 2002 ). `` Codon - anticodon interaction at the P site is a prerequisite for tRNA interaction with the small ribosomal subunit ''. The Journal of Biological Chemistry. 277 ( 21 ) : 19095 -- 105. doi : 10.1074 / jbc. M108902200. PMID 11867615. Jump up ^ WATSON, JD ( 1964 ). `` THE SYNTHESIS OF PROTEINS UPON RIBOSOMES ''. Bulletin de la Société de Chimie Biologique. 46 : 1399 -- 425. PMID 14270536. Jump up ^ Moazed, D ; Noller, HF ( 9 November 1989 ). `` Intermediate states in the movement of transfer RNA in the ribosome ''. Nature. 342 ( 6246 ) : 142 -- 8. doi : 10.1038 / 342142a0. PMID 2682263. Jump up ^ Agirrezabala, Xabier ; Lei, Jianlin ; Brunelle, Julie L. ; Ortiz - Meoz, Rodrigo F. ; Green, Rachel ; Frank, Joachim ( October 2008 ). `` Visualization of the Hybrid State of tRNA Binding Promoted by Spontaneous Ratcheting of the Ribosome ''. Molecular Cell. 32 ( 2 ) : 190 -- 197. doi : 10.1016 / j. molcel. 2008.10. 001. Jump up ^ Blanchard, SC ; Gonzalez, RL ; Kim, HD ; Chu, S ; Puglisi, JD ( October 2004 ). `` tRNA selection and kinetic proofreading in translation ''. Nature Structural & Molecular Biology. 11 ( 10 ) : 1008 -- 14. doi : 10.1038 / nsmb831. PMID 15448679. Jump up ^ Laursen, B.S. ; Sorensen, H.P. ; Mortensen, K.K. ; Sperling - Petersen, H.U. ( 8 March 2005 ). `` Initiation of Protein Synthesis in Bacteria ''. Microbiology and Molecular Biology Reviews. 69 ( 1 ) : 101 -- 123. doi : 10.1128 / MMBR. 69.1. 101 - 123.2005. Jump up ^ Wilson, JE ; Pestova, TV ; Hellen, CU ; Sarnow, P ( 18 August 2000 ). `` Initiation of protein synthesis from the A site of the ribosome ''. Cell. 102 ( 4 ) : 511 -- 20. doi : 10.1016 / s0092 - 8674 ( 00 ) 00055 - 6. PMID 10966112. Jump up ^ Yusupov, MM ; Yusupova, GZ ; Baucom, A ; Lieberman, K ; Earnest, TN ; Cate, JH ; Noller, HF ( 4 May 2001 ). `` Crystal structure of the ribosome at 5.5 A resolution ''. Science. 292 ( 5518 ) : 883 -- 96. doi : 10.1126 / science. 1060089. PMID 11283358. Jump up ^ Chopra, Shaileja ; Reader, John ( 25 December 2014 ). `` tRNAs as Antibiotic Targets ''. International Journal of Molecular Sciences. 16 ( 1 ) : 321 -- 349. doi : 10.3390 / ijms16010321. Jump up ^ Aoki, H. ; Ke, L. ; Poppe, S.M. ; Poel, T.J. ; Weaver, E.A. ; Gadwood, R.C. ; Thomas, R.C. ; Shinabarger, D.L. ; Ganoza, M.C. ( 1 April 2002 ). `` Oxazolidinone Antibiotics Target the P Site on Escherichiacoli Ribosomes ''. Antimicrobial Agents and Chemotherapy. 46 ( 4 ) : 1080 -- 1085. doi : 10.1128 / AAC. 46.4. 1080 - 1085.2002. Jump up ^ Johnston, Nicole ; Mukhtar, Tariq ; Wright, Gerard ( 1 August 2002 ). `` Streptogramin Antibiotics : Mode of Action and Resistance ''. Current Drug Targets. 3 ( 4 ) : 335 -- 344. doi : 10.2174 / 1389450023347678. Jump up ^ Champney, W. Scott ; Tober, Craig L. ( 21 August 2000 ). `` Specific Inhibition of 50S Ribosomal Subunit Formation in Staphylococcus aureus Cells by 16 - Membered Macrolide, Lincosamide, and Streptogramin B Antibiotics ''. Current Microbiology. 41 ( 2 ) : 126 -- 135. doi : 10.1007 / s002840010106. 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Question: peptidyl t rna is bound to which site
Answer: The P-site