Disulfide - wikipedia Disulfide In chemistry, a disulfide refers to a functional group with the structure R -- S -- S -- R '. The linkage is also called an SS - bond or sometimes a disulfide bridge and is usually derived by the coupling of two thiol groups. The connection is a persulfide, in analogy to its congener, peroxide ( R -- O -- O -- R ' ), but this terminology is rarely used, except in reference to hydrodisulfides ( R -- S -- S -- H or H -- S -- S -- H compounds ). In inorganic chemistry disulfide usually refers to the corresponding anion S2 − 2 ( S -- S ), for example in disulfur dichloride. A selection of disulfides FeS, `` fool 's gold '' S Cl, a common industrial chemical cystine, crosslinker in many proteins lipoic acid, a vitamin Ph S, a common organic disulfide Contents 1 Organic disulfides 1.1 Properties 1.2 Synthesis 1.3 Reactions 1.4 Occurrence in proteins 1.4. 1 Predicting disulfide abundance 1.4. 2 In Bacteria and Archaea 1.4. 3 In Eukaryotes 1.4. 4 Disulfides in regulatory proteins 1.4. 5 In hair and feathers 2 Inorganic disulfides 3 In industry 4 Related compounds 4.1 Misnomers 5 References 6 Further reading Organic disulfides Properties The disulfide bonds are strong, with a typical bond dissociation energy of 60 kcal / mol ( 251 kJ mol ). However, being about 40 % weaker than C -- C and C -- H bonds, the disulfide bond is often the `` weak link '' in many molecules. Furthermore, reflecting the polarizability of divalent sulfur, the S -- S bond is susceptible to scission by polar reagents, both electrophiles and especially nucleophiles : RS -- SR + Nu → RS -- Nu + RS The disulfide bond is about 2.05 Å in length, about 0.5 Å longer than a C -- C bond. Rotation about the S -- S axis is subject to a low barrier. Disulfides show a distinct preference for dihedral angles approaching 90 °. When the angle approaches 0 ° or 180 °, then the disulfide is a significantly better oxidant. Disulfides where the two R groups are the same are called symmetric, examples being diphenyl disulfide and dimethyl disulfide. When the two R groups are not identical, the compound is said to be an asymmetric or mixed disulfide. Although the hydrogenation of disulfides is usually not practical, the equilibrium constant for the reaction provides a measure of the standard redox potential for disulfides : RSSR + H → 2 RSH This value is about − 250 mV vs NHE ( pH = 7 ). By comparison, the standard reduction potential for ferrodoxins is about − 430 mV. Synthesis Disulfide bonds are usually formed from the oxidation of sulfhydryl ( -- SH ) groups, especially in biological contexts. The transformation is depicted as follows : 2 RSH ⇌ RS -- SR + 2 H + 2 e A variety of oxidants participate in this reaction including oxygen and hydrogen peroxide. Such reactions are thought to proceed via sulfenic acid intermediates. In the laboratory, iodine in the presence of base is commonly employed to oxidize thiols to disulfides. Several metals, such as copper ( II ) and iron ( III ) complexes affect this reaction. Alternatively, disulfide bonds in proteins often formed by thiol - disulfide exchange : RS -- SR + R ′ SH ⇌ R ′ S -- SR + RSH Such reactions are mediated by enzymes in some cases and in other cases are under equilibrium control, especially in the presence of a catalytic amount of base. The alkylation of alkali metal di - and polysulfides gives disulfides. `` Thiokol '' polymers arise when sodium polysulfide is treated with an alkyl dihalide. In the converse reaction, carbanionic reagents react with elemental sulfur to afford mixtures of the thioether, disulfide, and higher polysulfides. These reactions are often unselective but can be optimized for specific applications. Many specialized methods have been developed for forming disulfides, usually for applications in organic synthesis. Reagents that deliver the equivalent of `` RS '' react with thiols to give asymmetrical disulfides : RSH + R ′ SNR '' → RS -- SR ′ + HNR '', where R '' N = phthalimido Reactions The most important aspect of disulfide bonds is their cleavage, which occurs via reduction. A variety of reductants can be used. In biochemistry, thiols such as mercaptoethanol ( β - ME ) or dithiothreitol ( DTT ) serve as reductants ; the thiol reagents are used in excess to drive the equilibrium to the right : RS -- SR + 2 HOCH CH SH ⇌ HOCH CH S -- SCH CH OH + 2 RSH The reductant tris ( 2 - carboxyethyl ) phosphine ( TCEP ) is useful, beside being odorless compared to β - ME and DTT, because it is selective, working at both alkaline and acidic conditions ( unlike DTT ), is more hydrophilic and more resistant to oxidation in air. Furthermore, it is often not needed to remove TCEP before modification of protein thiols. In organic synthesis, hydride agents are typically employed for scission of disulfides, such as sodium borohydride. More aggressive, alkali metals will effect this reaction : RS -- SR + 2 Na → 2 NaSR These reactions are often followed by protonation of the resulting metal thiolate : NaSR + HCl → HSR + NaCl Thiol -- disulfide exchange is a chemical reaction in which a thiolate group -- S attacks a sulfur atom of a disulfide bond -- S -- S --. The original disulfide bond is broken, and its other sulfur atom ( green atom in Figure 1 ) is released as a new thiolate, carrying away the negative charge. Meanwhile, a new disulfide bond forms between the attacking thiolate ( red atom in Figure 1 ) and the original sulfur atom ( blue atom in Figure 1 ). Thiol -- disulfide exchange showing the linear intermediate in which the charge is shared among the three sulfur atoms. The thiolate group ( shown in red ) attacks a sulfur atom ( shown in blue ) of the disulfide bond, displacing the other sulfur atom ( shown in green ) and forming a new disulfide bond. Thiolates, not thiols, attack disulfide bonds. Hence, thiol -- disulfide exchange is inhibited at low pH ( typically, below 8 ) where the protonated thiol form is favored relative to the deprotonated thiolate form. ( The pK of a typical thiol group is roughly 8.3, but can vary due to its environment. ) Thiol -- disulfide exchange is the principal reaction by which disulfide bonds are formed and rearranged in a protein. The rearrangement of disulfide bonds within a protein generally occurs via intra-protein thiol -- disulfide exchange reactions ; a thiolate group of a cysteine residue attacks one of the protein 's own disulfide bonds. This process of disulfide rearrangement ( known as disulfide shuffling ) does not change the number of disulfide bonds within a protein, merely their location ( i.e., which cysteines are bonded ). Disulfide reshuffling is generally much faster than oxidation / reduction reactions, which change the number of disulfide bonds within a protein. The oxidation and reduction of protein disulfide bonds in vitro also generally occurs via thiol -- disulfide exchange reactions. Typically, the thiolate of a redox reagent such as glutathione or dithiothreitol attacks the disulfide bond on a protein forming a mixed disulfide bond between the protein and the reagent. This mixed disulfide bond when attacked by another thiolate from the reagent, leaves the cysteine oxidized. In effect, the disulfide bond is transferred from the protein to the reagent in two steps, both thiol -- disulfide exchange reactions. The in vivo oxidation and reduction of protein disulfide bonds by thiol -- disulfide exchange is facilitated by a protein called thioredoxin. This small protein, essential in all known organisms, contains two cysteine amino acid residues in a vicinal arrangement ( i.e., next to each other ), which allows it to form an internal disulfide bond, or disulfide bonds with other proteins. As such, it can be used as a repository of reduced or oxidized disulfide bond moieties. Many specialized organic reactions have been developed for disulfides, again mainly associated with the scission of the S -- S bond, which is usually the weakest bond in a molecule. In the Zincke disulfide cleavage reactions, disulfides are cleaved to give a sulfenyl halide by reaction with bromine or chlorine. Disulfide bonds ( schematic presentation ) in a protein. Occurrence in proteins Disulfide bonds play an important role in the folding and stability of some proteins, usually proteins secreted to the extracellular medium. Since most cellular compartments are reducing environments, in general, disulfide bonds are unstable in the cytosol, with some exceptions as noted below, unless a sulfhydryl oxidase is present. Cystine is composed of two cysteines linked by a disulfide bond ( shown here in its neutral form ). Disulfide bonds in proteins are formed between the thiol groups of cysteine residues by the process of oxidative folding. The other sulfur - containing amino acid, methionine, can not form disulfide bonds. A disulfide bond is typically denoted by hyphenating the abbreviations for cysteine, e.g., when referring to Ribonuclease A the `` Cys26 -- Cys84 disulfide bond '', or the `` 26 -- 84 disulfide bond '', or most simply as `` C26 -- C84 '' where the disulfide bond is understood and does not need to be mentioned. The prototype of a protein disulfide bond is the two - amino - acid peptide cystine, which is composed of two cysteine amino acids joined by a disulfide bond ( shown in Figure 2 in its unionized form ). The structure of a disulfide bond can be described by its χ dihedral angle between the C -- S -- S -- C atoms, which is usually close to ± 90 °. The disulfide bond stabilizes the folded form of a protein in several ways : It holds two portions of the protein together, biasing the protein towards the folded topology. That is, the disulfide bond destabilizes the unfolded form of the protein by lowering its entropy. The disulfide bond may form the nucleus of a hydrophobic core of the folded protein, i.e., local hydrophobic residues may condense around the disulfide bond and onto each other through hydrophobic interactions. Related to 1 and 2, the disulfide bond link two segments of the protein chain, the disulfide bond increases the effective local concentration of protein residues and lowers the effective local concentration of water molecules. Since water molecules attack amide - amide hydrogen bonds and break up secondary structure, a disulfide bond stabilizes secondary structure in its vicinity. For example, researchers have identified several pairs of peptides that are unstructured in isolation, but adopt stable secondary and tertiary structure upon forming a disulfide bond between them. A disulfide species is a particular pairing of cysteines in a disulfide - bonded protein and is usually depicted by listing the disulfide bonds in parentheses, e.g., the `` ( 26 -- 84, 58 -- 110 ) disulfide species ''. A disulfide ensemble is a grouping of all disulfide species with the same number of disulfide bonds, and is usually denoted as the 1S ensemble, the 2S ensemble, etc. for disulfide species having one, two, etc. disulfide bonds. Thus, the ( 26 -- 84 ) disulfide species belongs to the 1S ensemble, whereas the ( 26 -- 84, 58 -- 110 ) species belongs to the 2S ensemble. The single species with no disulfide bonds is usually denoted as R for `` fully reduced ''. Under typical conditions, disulfide reshuffling is much faster than the formation of new disulfide bonds or their reduction ; hence, the disulfide species within an ensemble equilibrate more quickly than between ensembles. The native form of a protein is usually a single disulfide species, although some proteins may cycle between a few disulfide states as part of their function, e.g., thioredoxin. In proteins with more than two cysteines, non-native disulfide species may be formed, which are almost always misfolded. As the number of cysteines increases, the number of nonnative species increases factorially. Predicting disulfide abundance The number of ways i in which p disulfide bonds can be formed from n cysteine residues present in a protein is given by the formula i = n ! ( n − 2 p ) ! p ! 2 p ( displaystyle i = ( \frac ( n ! ) ( ( n - 2p ) ! p ! 2 ^ ( p ) ) ) ) Here, i is the number of different disulfide bond isomers or connectivities possible. n is the number of cysteines present in the protein molecule. p is the number of disulphide bonds that are formed ( hence 2p is less than or equal to n ). The above formula is the most general relation which can be used to calculate the number of possible disulfide bond isomers ( or connectivities ) when n is either even or odd, and when all or only some of the cysteines are involved in the formation of disulfide bonds. However, many of the naturally occurring proteins that have disulfide bonds possess an even number of cysteines with all of the cysteines participating in the formation of disulfide bonds. For this specific case, n is an even number and p is equal to n / 2. Substituting the value of p, the above formula for the possible number of disulfide bond connectivities simplifies to : i = n ! ( n − 2 p ) ! p ! 2 p = n ! ( n − 2 ( n 2 ) ) ! ( n 2 ) ! 2 ( n 2 ) = n ! ( n 2 ) ! 2 ( n 2 ) ( displaystyle i = ( \frac ( n ! ) ( ( n - 2p ) ! p ! 2 ^ ( p ) ) ) = ( \frac ( n ! ) ( ( n - 2 ( ( \frac ( n ) ( 2 ) ) ) ) ! ( ( ( \frac ( n ) ( 2 ) ) ) ) ! ( 2 ^ ( ( ( \frac ( n ) ( 2 ) ) ) ) ) ) ) = ( \frac ( n ! ) ( ( ( ( \frac ( n ) ( 2 ) ) ) ) ! ( 2 ^ ( ( ( \frac ( n ) ( 2 ) ) ) ) ) ) ) ) For this particular case ( n is even and all cysteines form disulfide bonds ), a formula which is more easier to remember is given by : i = ( n − 1 ) ( n − 3 ) ( n − 5 )... 1 ( displaystyle i = ( n - 1 ) ( n - 3 ) ( n - 5 ) ... 1 ) Both of the above relations hold good for the proteins which have an even number of cysteines and all the cysteines are involved in the formation of disulfide bonds. Both of the above formulae are derived using the same logic and essentially represent a simplification of the same starting formula. As a specific example for the above case, an eight - cysteine protein such as ribonuclease A can form 105 different four - disulfide species when all the cysteines are involved in the formation of disulfide bonds. Here n = 8 and p = 4. So, here i = ( 8 − 1 ) ( 8 − 3 ) ( 8 − 5 ) ( 8 − 7 ) = 7 ⋅ 5 ⋅ 3 ⋅ 1 = 105 ( displaystyle i = ( 8 - 1 ) ( 8 - 3 ) ( 8 - 5 ) ( 8 - 7 ) = 7 cdot 5 cdot 3 cdot 1 = 105 ) Only one of the 105 possible isomers is the native disulfide species. Isomerases have been identified that catalyze the interconversion of disulfide species, accelerating the formation of the native disulfide species. Disulfide species that have only native disulfide bonds ( but not all of them ) are denoted by des followed by the lacking native disulfide bond ( s ) in square brackets. For example, the des ( 40 -- 95 ) disulfide species has all the native disulfide bonds except that between cysteines 40 and 95. Disulfide species that lack one native disulfide bond are frequently folded, in particular, if the missing disulfide bond is exposed to solvent in the folded, native protein. In order to analyze the structure of proteins, it is often necessary to break disulfide bonds. This reduction of disulfide bonds can be accomplished by treatment with 2 - mercaptoethanol, dithiothreitol, or tris ( 2 - carboxyethyl ) phosphine. In bacteria and archaea Disulfide bonds play an important protective role for bacteria as a reversible switch that turns a protein on or off when bacterial cells are exposed to oxidation reactions. Hydrogen peroxide ( H O ) in particular could severely damage DNA and kill the bacterium at low concentrations if not for the protective action of the SS - bond. Archaea typically have fewer disulfides than higher organisms. In eukaryotes In eukaryotic cells, in general, stable disulfide bonds are formed in the lumen of the RER ( rough endoplasmic reticulum ) and the mitochondrial intermembrane space but not in the cytosol. This is due to the more oxidizing environment of the aforementioned compartments and more reducing environment of the cytosol ( see glutathione ). Thus disulfide bonds are mostly found in secretory proteins, lysosomal proteins, and the exoplasmic domains of membrane proteins. There are notable exceptions to this rule. For example, many nuclear and cytosolic proteins can become disulfide - crosslinked during necrotic cell death. Similarly, a number of cytosolic proteins which have cysteine residues in proximity to each other that function as oxidation sensors or redox catalysts ; when the reductive potential of the cell fails, they oxidize and trigger cellular response mechanisms. Vaccinia virus also produces cytosolic proteins and peptides that have many disulfide bonds ; although the reason for this is unknown presumably they have protective effects against intracellular proteolysis machinery. Disulfide bonds are also formed within and between protamines in the sperm chromatin of many mammalian species. Disulfides in regulatory proteins As disulfide bonds can be reversibly reduced and re-oxidized, the redox state of these bonds has evolved into a signaling element. In chloroplasts, for example, the enzymatic reduction of disulfide bonds has been linked to the control of numerous metabolic pathways as well as gene expression. The reductive signaling activity has been shown, thus far, to be carried by the ferredoxin thioredoxin system, channeling electrons from the light reactions of photosystem I to catalytically reduce disulfides in regulated proteins in a light dependent manner. In this way chloroplasts adjust the activity of key processes such as the Calvin -- Benson cycle, starch degradation, ATP production and gene expression according to light intensity. In hair and feathers Over 90 % of the dry weight of hair comprises proteins called keratins, which have a high disulfide content, from the amino acid cysteine. The robustness conferred in part by disulfide linkages is illustrated by the recovery of virtually intact hair from ancient Egyptian tombs. Feathers have similar keratins and are extremely resistant to protein digestive enzymes. Different parts of the hair and feather have different cysteine levels, leading to harder or softer material. Manipulating disulfide bonds in hair is the basis for the permanent wave in hairstyling. Reagents that affect the making and breaking of S -- S bonds are key, e.g., ammonium thioglycolate. The high disulfide content of feathers dictates the high sulfur content of bird eggs. The high sulfur content of hair and feathers contributes to the disagreeable odor that results when they are burned. Inorganic disulfides The disulfide anion is S2 − 2, or S -- S. Sulfur is usually assigned to the reduced oxidation number − 2, described as S and called sulfide. It has the electron configuration of a noble gas ( argon ). In disulfide, sulfur is only reduced to a state with oxidation number − 1. Its configuration then resembles that of a chlorine atom. It thus tends to form a covalent bond with another S center to form S2 − 2 group. Oxygen also behaves similarly, e.g. in peroxides such as H O. Examples : Iron disulfide ( FeS ), e.g. the mineral pyrite ( V ( S ) -- patronite ). Disulfur dichloride ( S Cl ), a distillable liquid. In industry Disulfide and ( polysulfide ) bonds are the crosslinking groups that result from the vulcanization of rubber. In analogy to the role of disulfides in proteins, the S -- S linkages in rubber are crosslinkers, and strongly affect the rheology of the material. Related compounds Thiosulfoxides are orthogonally isomeric with disulfides, having the second sulfur branching from the first and not partaking in a continuous chain, i.e. > S = S rather than -- S -- S --. Disulfide bonds are analogous but more common than related peroxides and diselenide bonds. Intermediate compounds of these also exist, for example, thioperoxides, also known as oxaulfide bonds, have formula R OSR ( equivalently R SOR ). These are isomeric to sulfoxides in a similar manner to the above ; i.e. > S =O rather than -- S -- O --. Thiuram disulfides, with the formula ( R NC ( S ) S ), are disulfides but they behave distinctly because of the thiocarbonyl group. Compounds with three sulfur atoms, e.g., CH S -- S -- SCH, are called trisulfides, or trisulfide bonds. Misnomers Disulfide is also used to refer to compounds that contain two sulfide ( S ) centers. The compound carbon disulfide, CS is described with the structural formula i.e. S = C = S. This molecule is not a disulfide in the sense that it lacks a S-S bond. Similarly, molybdenum disulfide, MoS, is not a disulfide in the sense again that its sulfur atoms are not linked. CS MoS References This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. ( September 2015 ) Jump up ^ R.J. Cremlyn An Introduction to Organosulfur Chemistry John Wiley and Sons : Chichester ( 1996 ). ISBN 0 - 471 - 95512 - 4. ^ Jump up to : Sevier, C.S. ; Kaiser, C.A. ( 2002 ). `` Formation and transfer of disulphide bonds in living cells ''. Nature Reviews Molecular Cell Biology. 3 ( 11 ) : 836 -- 847. PMID 12415301. doi : 10.1038 / nrm954. ^ Jump up to : Witt, D. ( 2008 ). `` Recent developments in disulfide bond formation ''. Synthesis. 2008 ( 16 ) : 2491 -- 2509. doi : 10.1055 / s - 2008 - 1067188. Jump up ^ TCEP technical information, from Interchim Jump up ^ Gilbert HF ( 1990 ). `` Molecular and Cellular Aspects of Thiol -- Disulfide Exchange ''. Advances in Enzymology. 63 : 69 -- 172. PMID 2407068. doi : 10.1002 / 9780470123096. ch2. Jump up ^ Gilbert HF ( 1995 ). `` Thiol / disulfide exchange equilibria and disulfide bond stability ''. Methods in Enzymology. 251 : 8 -- 28. PMID 7651233. doi : 10.1016 / 0076 - 6879 ( 95 ) 51107 - 5. Jump up ^ For example the conversion of di - o - nitrophenyl disulfide to o - nitrophenylsulfur chloride Max H. Hubacher ( 1943 ). `` o - Nitrophenylsulfur chloride ''. Org. Synth. ; Coll. Vol., 2, p. 455 Jump up ^ Methyl sulfenyl chloride : Irwin B. Douglass and Richard V. Norton ( 1973 ). `` Methanesulfinyl Chloride ''. Org. Synth. ; Coll. Vol., 5, p. 709 Jump up ^ Hatahet F, Nguyen VD, Salo KE, Ruddock LW ( 2010 ). `` Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli ''. MCF. 9 ( 67 ) : 67. PMC 2946281. PMID 20836848. doi : 10.1186 / 1475 - 2859 - 9 - 67. Jump up ^ Ruoppolo M, Vinci F, Klink TA, Raines RT, Marino G ( 2000 ). `` Contribution of individual disulfide bonds to the oxidative folding of ribonuclease A ''. Biochemistry. 39 ( 39 ) : 12033 -- 42. PMID 11009618. doi : 10.1021 / bi001044n. Jump up ^ Ladenstein, R. ; Ren, B. ( 2008 ). `` Reconsideration of an early dogma, saying `` there is no evidence for disulfide bonds in proteins from archaea '' ``. Extremophiles. 12 ( 1 ) : 29 -- 38. PMID 17508126. doi : 10.1007 / s00792 - 007 - 0076 - z. Jump up ^ Samson, Andre L. ; Knaupp, Anja S. ; Sashindranath, Maithili ; Borg, Rachael J. ; Au, Amanda E. - L. ; Cops, Elisa J. ; Saunders, Helen M. ; Cody, Stephen H. ; McLean, Catriona A. ( 2012 - 10 - 25 ). `` Nucleocytoplasmic coagulation : an injury - induced aggregation event that disulfide crosslinks proteins and facilitates their removal by plasmin ''. Cell Reports. 2 ( 4 ) : 889 -- 901. ISSN 2211 - 1247. PMID 23041318. doi : 10.1016 / j. celrep. 2012.08. 026. Further reading Sela M, Lifson S ( 1959 ). `` The reformation of disulfide bridges in proteins ''. Biochim Biophys Acta. 36 ( 2 ) : 471 -- 78. PMID 14444674. doi : 10.1016 / 0006 - 3002 ( 59 ) 90188 - X. Stark GR. ; Stern, K ; Atala, A ; Yoo, J ( 1977 ). `` Cleavage at cysteine after cyanylation ''. Methods Enzymol. 47 ( 2 ) : 129 -- 32. PMID 927170. doi : 10.1016 / j. ymeth. 2008.09. 005. Thornton JM. ( 1981 ). `` Disulphide bridges in globular proteins ''. J Mol Biol. 151 ( 2 ) : 261 -- 87. PMID 7338898. doi : 10.1016 / 0022 - 2836 ( 81 ) 90515 - 5. Thannhauser TW, Konishi Y, Scheraga HA ( 1984 ). `` Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins ''. Anal Biochem. 138 ( 1 ) : 181 -- 8. PMID 6547275. doi : 10.1016 / 0003 - 2697 ( 84 ) 90786 - 3. Wu J, Watson JT ( 1998 ). `` Optimization of the cleavage reaction for cyanylated cysteinyl proteins for efficient and simplified mass mapping ''. Anal Biochem. 258 ( 2 ) : 268 -- 76. PMID 9570840. doi : 10.1006 / abio. 1998.2596. Futami J, Tada H, Seno M, Ishikami S, Yamada H ( 2000 ). `` Stabilization of human RNase 1 by introduction of a disulfide bond between residues 4 and 118 ''. J Biochem. 128 ( 2 ) : 245 -- 50. PMID 10920260. doi : 10.1093 / oxfordjournals. jbchem. a022747. Wittenberg, G ; Danon, A ( 2008 ). `` Disulfide bond formation in chloroplasts : Formation of disulfide bonds in signaling chloroplast proteins ''. Plant Science. 175 ( 4 ) : 459 -- 66. doi : 10.1016 / j. plantsci. 2008.05. 011. Kadokura, Hiroshi ; Katzen, Federico ; Beckwith, Jon ( 2003 ). `` Protein Disulfide Bond Formation in Prokaryotes ''. Annual Review of Biochemistry. 72 ( 1 ) : 111 -- 135. PMID 12524212. doi : 10.1146 / annurev. biochem. 72.121801. 161459. Tu, B.P. ; Weissman, JS ( 2004 ). `` Oxidative protein folding in eukaryotes : mechanisms and consequences ''. The Journal of Cell Biology. 164 ( 3 ) : 341 -- 46. PMC 2172237. PMID 14757749. doi : 10.1083 / jcb. 200311055. Ellgaard, Lars ; Ruddock, Lloyd W. ( 2005 ). `` The human protein disulphide isomerase family : substrate interactions and functional properties ''. EMBO Reports. 6 ( 1 ) : 28 -- 32. PMC 1299221. PMID 15643448. doi : 10.1038 / sj. embor. 7400311. Functional groups Only carbon, hydrogen and oxygen Hydrocarbons Allene Alkene ( Allyl, Vinyl ) Alkyl ( Methyl, Ethyl, Propyl, Butyl, Pentyl ) Alkyne Benzyl Carbene Cumulene Methylene bridge Methylene group Methine Phenyl Other Acetoxy Acetyl Acryloyl Acyl Aldehyde Alkoxy ( Methoxy ) Benzoyl Carbonyl Carboxyl Dioxirane Epoxide Ester Ether Ethylenedioxy Hydroxy Ketone Methylenedioxy Peroxide ( Organic ) Ynone Only one element apart from C, H, O Nitrogen Amine Azo compound Cyanate Hydrazone Imide Imine Isocyanate Isonitrile Nitrene Nitrile Nitro compound Nitroso compound Organic amide Oxime Phosphorus Phosphonate Phosphonous Sulfur Disulfide Sulfone Sulfonic acid Sulfoxide Thial Thioester Thioether Thioketone Thiol Selenium Selenol Selenonic acid Seleninic acid Selenenic acid Tellurium Tellurol Other Isothiocyanate Phosphoramide Sulfenyl chloride Sulfonamide Thiocyanate See also chemical classification, chemical nomenclature ( inorganic, organic ) Protein primary structure and posttranslational modifications General Peptide bond Protein biosynthesis Proteolysis Racemization N-O acyl shift N terminus Acetylation Carbamylation Formylation Glycation Methylation Myristoylation ( Gly ) C terminus Amidation Glycosyl phosphatidylinositol ( GPI ) O - methylation Detyrosination Single specific AAs Serine / Threonine Phosphorylation Dephosphorylation Glycosylation Methylidene - imidazolone ( MIO ) formation Tyrosine Phosphorylation Dephosphorylation Sulfation Porphyrin ring linkage Adenylylation Flavin linkage Topaquinone ( TPQ ) formation Detyrosination Cysteine Palmitoylation Prenylation Aspartate Succinimide formation Glutamate Carboxylation Methylation Polyglutamylation Polyglycylation Asparagine Deamidation Glycosylation Glutamine Transglutamination Lysine Methylation Acetylation Acylation Adenylylation Hydroxylation Ubiquitination Sumoylation ADP - ribosylation Deamination Oxidative deamination to aldehyde O - glycosylation Imine formation Glycation Carbamylation Succinylation Arginine Citrullination Methylation ADP - ribosylation Proline Hydroxylation Histidine Diphthamide formation Adenylylation Tryptophan C - mannosylation Crosslinks between two AAs Cysteine - Cysteine Disulfide bond Methionine - Hydroxylysine Sulfilimine bond Lysine - Tyrosylquinone Lysine tyrosylquinone ( LTQ ) formation Tryptophan - Tryptophylquinone Tryptophan tryptophylquinone ( TTQ ) formation Three consecutive AAs ( chromophore formation ) Serine -- Tyrosine -- Glycine p - Hydroxybenzylidene - imidazolinone formation Histidine -- Tyrosine -- Glycine 4 - ( p - hydroxybenzylidene ) - 5 - imidazolinone formation Crosslinks between four AAs Allysine - Allysine - Allysine - Lysine Desmosine Retrieved from `` https://en.wikipedia.org/w/index.php?title=Disulfide&oldid=786660895 '' Categories : Organic disulfides Protein structure Posttranslational modification Sulfur Functional groups Hidden categories : All articles with unsourced statements Articles with unsourced statements from November 2012 Wikipedia articles needing clarification from April 2016 Articles needing additional references from September 2015 All articles needing additional references Talk Contents About Wikipedia Čeština Deutsch Español Français Hrvatski Italiano Bahasa Melayu 日本 語 Polski Português Српски / srpski Srpskohrvatski / српскохрватски Suomi 中文 Edit links This page was last edited on 20 June 2017, at 20 : 06. Text is available under the Creative Commons Attribution - ShareAlike License ; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia ® is a registered trademark of the Wikimedia Foundation, Inc., a non-profit organization. About Wikipedia

Question: the conversion of a thiol into a disulfide occurs by what type of chemical reaction
Answer: coupling of two thiol groups