Tetrazolium chloride - wikipedia Tetrazolium chloride Tetrazolium chloride Names IUPAC name 2, 3, 5 - Triphenyl - 2H - tetrazolium chloride Other names TTC Identifiers CAS Number 298 - 96 - 4 3D model ( JSmol ) Interactive image ChEBI CHEBI : 78019 ChemSpider 8926 ECHA InfoCard 100.005. 520 InChI InChI = 1S / CH2N4. ClH / c1 - 2 - 4 - 5 - 3 - 1 ; / h1H, ( H, 2, 3, 4, 5 ) ; 1H Key : JXCNUCFTHLGXDQ - UHFFFAOYSA - N InChI = 1 / CH2N4. ClH / c1 - 2 - 4 - 5 - 3 - 1 ; / h1H, ( H, 2, 3, 4, 5 ) ; 1H Key : JXCNUCFTHLGXDQ - UHFFFAOYAY SMILES ( Cl - ). n1c ( nH+ ) nn1 Properties Chemical formula C H ClN Molar mass 334.8 g / mol Appearance white crystalline powder Solubility in water Soluble Solubility in water ~ 1 mg / ml Solubility in PBS ( pH 7.2 ) ~ 1 mg / ml Solubility in ethanol ~ 1 mg / ml Solubility in DMSO ~ 0.25 mg / ml log P − 2.4 Hazards GHS pictograms GHS signal word Danger GHS hazard statements H228 GHS precautionary statements P210 NFPA 704 0 0 Lethal dose or concentration ( LD, LC ) : LD ( median dose ) 5.6 mg / kg Except where otherwise noted, data are given for materials in their standard state ( at 25 ° C ( 77 ° F ), 100 kPa ). N verify ( what is ? ) Infobox references Triphenyl tetrazolium chloride, TTC, or simply tetrazolium chloride ( with the formula 2, 3, 5 - triphenyl - 2H - tetrazolium chloride ) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration. It is a white crystalline powder, soluble in water, ethanol and acetone but insoluble in ether. Contents 1 TTC assay 2 See also 3 References 4 External links TTC assay In the TTC assay ( also known as TTC test or tetrazolium test ), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF ( 1, 3, 5 - triphenylformazan ) in living tissues due to the activity of various dehydrogenases ( enzymes important in oxidation of organic compounds and thus cellular metabolism ), while it remains in its unreacted state in areas of necrosis since these enzymes have either denatured or degraded. TTC has been employed in autopsy pathology to assist post-mortem identification of myocardial infarctions. Healthy viable heart muscle will stain deep red from the cardiac lactate dehydrogenase, while areas of potential infarctions will be more pale. See also MTT assay Seed testing References Jump up ^ Witty M. ( 2012 ). Topographischer Nachweis der Keimfähigkeit der Getreidefrüchte durch Tetrazoliumsalze ( Topographic Detection of Germination in Cereal Crops by Tetrazolium Salts ) -- A Translation of Lakon 's 1942 Paper on Tetrazolium Seed Testing. Seed Technology 34 ( 2 ) : 275 - 282. Jump up ^ Witty M. ( 2012 ). The process for 2, 3, 5 - triphenyl -- tetrazolium chloride synthesis, an intellectual property seized immediately after world war II. Bulletin for the History of Chemistry 37 ( 2 ) : 91 - 95. External links Retrieved from `` https://en.wikipedia.org/w/index.php?title=Tetrazolium_chloride&oldid=843609312 '' Categories : Chlorides Redox indicators Tetrazoles Hidden categories : Chemicals without a PubChem CID Articles without KEGG source Articles without UNII source Articles with changed EBI identifier Articles with changed ChemSpider identifier ECHA InfoCard ID from Wikidata Chembox having GHS data Articles containing unverified chemical infoboxes Talk Contents About Wikipedia تۆرکجه Deutsch Español فارسی Français 日本 語 Српски / srpski Srpskohrvatski / српскохрватски Edit links This page was last edited on 30 May 2018, at 07 : 50 ( UTC ). Text is available under the Creative Commons Attribution - ShareAlike License ; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia ® is a registered trademark of the Wikimedia Foundation, Inc., a non-profit organization. About Wikipedia

Question: ttc can be converted to insoluble red formazan by the activity of
Answer: various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism)