Infection by BTV is acid-activated and requires the low pH within endosomes for disassembly of the outer viral capsid and membrane penetration, and BTV particles have been described inside endosomes which have the appearance of clathrin-coated vesicles [48] . Recent studies have concluded that entry and infection of Vero and Hela cells by BTV-10 occurs via CME, with capsid disassembly and membrane penetration within earlyendosomes [45] . Here we describe the use of pharmacological and DN inhibitors of endocytosis to investigate entry and infection of BHK cells by BTV-1. We found that the clathrin pathway is not the major entry route used by BTV-1 to infect BHK cells. Instead we found that the entry mechanism shares certain characteristics in common with macropinocytosis and appears to deliver virus directly to late endosomal compartments. These studies extend earlier observations and show that BTV joins an increasing number of viruses that can exploit multiple endocytosis pathways for infectious entry.