For in vivo experiments, we selected a high generation xenograft that showed both angiogenic and invasive features and recapitulated the histology of the patient lesion. Furthermore, this xenograft showed a high level of nestin expression similar to the patient material. In translational research it is crucial to assure that the experimental tumors truly reflect the corresponding patient's tumor properties to avoid using non-relevant animal models. However, this strategy is not common practice yet, based on the simplicity of using established cell lines for in vivo experiments.