The recent application of electron tomography (ET) to the study of virus infection has allowed 3D evaluation of virus replication [48] [49] [50] [51] [52] [53] including in mosquito-borne flavivirus infection [30, 31] . We employed ET of 250 nm thick, fixed, and resin-embedded specimens to enhance the findings described in the earlier sections. Tilt series were acquired, aligned using gold particles, and tomograms assembled to reveal 3D structures. In Vero cells acutely infected with LGTV, we found the vesicles and virions were contained within an anastomosing network of dilated membranes. The 60-100 nm diameter vesicles were round in shape ( Fig. 8A and Movie S1) and there were pore-like openings connecting vesicles to the cytoplasm (Fig. 9A ) and to other vesicles (Fig. 9D) .