The application of the Endo IV cleavage as a post-PCR detection system is shown in Figures 8 and 9 . Following an asymmetric PCR using primers to produce predominantly single-stranded amplicon, two allele-specific probes, an enhancer and Endo IV enzyme are added to the amplification mixture and incubated isothermally for about an hour. The cleaved fluorescence from the two allele-specific probes, each labeled with a different fluorescent dye, is then plotted in a scatter diagram as shown in Figures 8 and 9 for the genotyping analysis of two single nucleotide polymorphisms (SNPs) which are of immediate importance to in cancer (http:// snp500cancer.nci.nih.gov). The scatter diagrams of two polymorphisms, namely agouti signal molecular epidemiology studies protein (ASIP-01) and adenomatous polyposis coli (APC-03) (http://snp500cancer.nci.nih.gov) are shown in Figures 8 and 9 , respectively. In both cases, a probe specific to the wild-type allele is labeled with FL1 and a probe specific for the mutant allele labeled with FL3 is used. The probe sets, designed to analyze ASIP-01 and APC-03 polymorphisms, were used to assay 102 unrelated human DNA samples obtained from the Coriell Institute. In the case of the ASIP-01 target the mismatch was situated two bases from the 3 0 end of the probe, while in the case of APC-03, the mismatch was six bases from the 3 0 end of the probe.