We used rAAV2/1-Ova and rAAV2/8-Ova administration to model a gene therapy setting where the transgene encodes for a highly immunogenic secreted protein. We first evaluated the capacity of these vectors to transduce muscle cells upon direct i.m. injection. Injection of 10 11 viral genome (vg) rAAV2/1-Ova or rAAV2/8-Ova similarly resulted in efficient transduction, as attested by the detection of Ova DNA and mRNA in the injected muscles quantified by qPCR and qRT-PCR respectively ( Figure 1A) . No significant difference was found when comparing the transduction efficiency of rAAV2/1-Ova and rAAV2/8-Ova in these conditions.