To confirm expression of the V5/N-term protein in cell culture capped RNA was synthesised from the JV V5 T7 cDNA construct, which was used to transfect CRFK cells. As there is currently no host cell line in which to propagate JV the CRFK cell line was used as it has been shown to support the replication of feline calicivirus [30] . Confocal immunofluorescence of transfected cells using the anti-V5 antibody demonstrated expression of the V5/Nterm protein in cultured cells ( Figure 6 ). Expression of the V5/Nterm protein was diffuse and did not co-localise with the Golgi/ ER/plasma membrane marker wheat germ agglutinin (WGA) and therefore displays a different pattern of cellular expression compared to Norwalk virus [15] . Cells transfected with the wild type full length JV RNA were negative for fluorescence (data not shown).