The nucleocapsid structure implies equimolar ratios of NP, VP24, VP30, and VP35. A genome of 18.9 kbp with 13 bases per NP with a single genome copy gives 1454 molecules of NP, VP24, VP30, and VP35 per virion. Previous analyses of Coomassie blue stained gels of purified EBOV predicted 625, 1208, 833, and 2686 protein molecules of NP, VP24, VP30, and VP35, per virion respectively [38] , which is in the same stoichiometric range as predicted by our model for the nucleocapsid, taking into account the variability of individual protein band staining by Coomassie blue, which is affected by factors such as distance of migration [39] , basic amino acid content [40] , and the extent of glycosylation [41] . Since NP is glycosylated, we anticipate underestimation of the NP content of virions [14, 41] .