Since a relatively unbiased selection scheme for producing hMAbs from donor 033 indicated that most hMAbs were crossreactive, weakly neutralizing and directed to antigens other than rE, we altered the selection scheme for donor 013 to enrich for hMAbs that recognized rE from DENV2. After the initial screening of memory B cell culture supernatants, the relatively rare rE protein binding cultures were selected for cloning and expansion. Ten rE binding hMAbs were produced (all IgG1). When the antibodies were tested for binding to heterologous serotypes, 5 hMAbs were DENV2 type-specific, 2 hMAbs were dengue subcomplexspecific and 3 hMAbs were dengue complex-specific ( Figure 3A) . Six of the ten hMAbs bound to EDIII from DENV2 ( Figure 3A ).