Finally, to check whether rRNA destruction during apoptotic response is not due to cessation of translation in dying cells, cells were treated for 200 min with the translation elongation inhibitor cycloheximide (200 mg/ml) and this did not lead to an apparent rRNA degradation (Supplementary Figure S1A) . This is also supported by our earlier observations that exposure of yeast cells to many oxidative agents that cause cell death does not result in rRNA decay ( Figure 1A) .