Oligonucleotide capture of O. volvulus DNA Pools of spiked heads and bodies were prepared as described above. The head and body pools were homogenized in 500 ml of 10 mM Tris-HCl (pH 8.0) 1 mM EDTA, and proteinase K added to a final concentration of 2 mg/ml. The homogenates were incubated at 56uC for 2 hours, and dithiothreitol added to a final concentration of 20 mM. The samples were heated to 100uC for 30 minutes and subjected to three freeze-thaw cycles. The homogenates were subjected to centrifugation at 13,4006g for 5 minutes and the supernatant placed into a new tube. The solutions were brought to a final concentration of 100 mM Tris-HCl (pH 7.5) 100 mM NaCl. A total of 5 ml of a 0.5 mM solution of OVS2-biotin primer (59B-AATCTCAAAAAACGGGTA-CATA-39, where B = biotin) was added to each sample. The samples were then heated to 95uC for three minutes and allowed to cool slowly to room temperature.