Thirty differentially expressed serum proteins identified in FM patients (compared to HC) were subjected to functional pathway analysis using Ingenuity Pathway Analysis (IPA). Out of those 30 candidates, 27 were eligible for network analysis (focus molecule) based on the IPA Knowledge Base criteria. Two overlapping interaction networks were identified where the highest scoring network included 14 out of the 27 focus molecules, while the second network included 10 focus molecules ( Figure S6 ; Table  S5 .1). The most significant related functions derived from these overlapping networks included, lipid metabolism (14 proteins, p = 2.92E 209 -5.48E 203 ), inflammatory response (18 proteins, p = 1.07E 211 -5.48E 203 ), molecular transport (15 proteins, p = 2.92E 209 -5.48E 203 ), immune cell trafficking (10 proteins, p = 9.32E 208 -4.12E 203 ) and humoral immune response (7 proteins, p = 1.10E 205 -5.48E 203 ). According to this functional pathway analysis, Pf infection leads to the alteration of multiple serum proteins involved in diverse essential physiological pathways, including acute phase response (Ratio = 0.067, p = 1.11E 218 ) and primary immunodeficiency signaling (ratio = 0.048, p = 5.1E 205 ) (Table S5. 2). Functional analysis of differentially expressed proteins was also performed using Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) databases. In PANTHER analysis blood coagulation system (p = 4.88E 205 ) was again identified. Moreover, the heterotrimeric G-protein signaling, interleukin signaling pathway and inflammation mediated by chemokine and cytokine signaling pathways were identified as related physiological pathways with statistical significance (p,0.05) (Table S5. 3). Further, DAVID analysis also confirmed modulation of complement and coagulation cascades (p = 1.28E 204 ) in FM (Table S5. 