3A, right-hand graph), although no apparent influence was This observation clearly demonstrates that leucine is observed on serylation ( Figure 3A , left-hand graph). These definitely attached to the tRNA possessing G73; the tRNA findings strongly indicate that the methyl group of m 1 G37 therefore must be tRNA Ser CAG and not tRNA Leu . Thus, plays a crucial role in enhancing the leucylation activity it is concluded that the tRNA which incorporated leucine of tRNA Ser CAG. in vitro is in fact tRNA Ser CAG. This deduction is supported