Our group analyzed recently HRV-39 genome evolution in experimental immunocompetent human infections [37] . We estimated an in vivo mutation frequency of 3.4610 24 mutations/ nucleotides over the entire open reading frame (ORF) during a five-day acute infection period, and identified regions of mutation hot spots in the viral capsid (VP1, VP2 and VP3), and 2C and 3C genes.