The emerging picture from systems biology approaches is that eukaroyotic translation elongation factors (eEFs), such as eEF1A, play several roles during TBSV replication. Accordingly, eEF1A has been shown to facilitate the assembly of the viral replicase complex and stimulate the initiation of minus-strand synthesis by the viral RNA-dependent RNA polymerase (RdRp) [23, 24] . Another translation elongation factor identified in our genomewide screens with TBSV is eukaryotic elongation factor 1Bgamma (eEF1Bc) [15] . eEF1Bc is an abundant, but not essential cellular protein, which is part of the eukaryotic translation elongation factor 1B complex also containing the eEF1Ba subunit in yeast and the eEF1Ba and eEF1Bd subunits in metazoans [28] .The eEF1B complex is the guanine nucleotide exchange factor for eEF1A, which binds and delivers aminoacyl-tRNA in the GTPbound form to the elongating ribosome. Additional roles have been ascribed to eEF1Bc in vesicle-mediated intracellular protein transport, RNA-binding, vacuolar protein degradation, oxidative stress, intermediate filament interactions and calcium-dependent membrane-binding [29, 30, 31] .