IUPred uses a cutoff of 0.5 to classify a sequence region as either structured or disordered, with values above this threshold corresponding to disorder, highlighted in green background and lower values indicating structured regions, displayed in red background in the output graph. Disorder and domain information is combined by Motifs, present in proteins in several repeats, which mediate binding to the hydrophobic cleft created by subdomains 1 and 3 of G-actin LIG_Actin_WH2_2 LIG_Actin_RPEL_3