The chimeric CGMMV sequence analyses showed one common similarity (Figures 6 and 7) , that is, that part of the read-through amber stops codon sequence, and additional nucleotides "CC-AAA-TAG" were retained for all sequenced clones. This suggests that deletion had occurred within the host plant. The sequence analysis also showed that the EB4 was not fully expressed in the putative chimeric CGMMV. : Sequence alignments of cloned RT-PCR-amplified products from putative chimeric CGMMV RNA at 30 dpi. Plasmid clones sent for sequence analysis were pR P3U1, pR P3U3, pR P3U4, and pR P3U11. The sequence alignment shows that EB4 sequence was not within the insert of plasmids, except for a partial sequence for pR P3U4 clone. Only part of the downstream sequence of EB4 was detected together with 3 untranslated region of CGMMV for pR P3U4 clone, the rest were without EB4 sequence. Introduced read-through sequences and extra codons are underlined. Complete EB4 sequence is aligned accordingly with the other sequences. The positive results from the initial RT-PCR screening of transcript-inoculated plants at 14 dpi ( Figure 4 ) suggest the presence of EB4. The EB4 was, however, not detectable at 30 dpi, and only present in some (< 50% tested) plants at 21 dpi. These findings strongly suggest that deletion had occurred within the host plants after 14 dpi. The sequence analyses in this section are summarized in Table 3 .