To overcome disadvantages of a natural enzyme, many methods was applied to obtain artificial evolution enzymes, and this approach is not only faster than natural evolution but also provides a deeper understanding of enzyme evolution. Several methods of designing new enzymes are available, and gene sequence truncation is also investigated for its effects on enzymatic properties. The non-conservative N-terminal domain of the protein phosphatase1 (PP1), with 1-8 residues deleted, showed higher sensitivity to three substrates and influenced the structure and properties of PP1 [10] , whereas the truncation of the Cterminal region improved the thermal stability of endo-bglucanase from Bacillus subtilis JA18 [11] . However, the loss of the C-terminal regulatory domain resulted in a loss of the ability to catalyze the aldol reaction [12] .