We have used the C. albicans cell surface microarray to evaluate the antibody profile of patients with candidemia against healthy individuals and uninfected hospital patients to determine relevant cell surface antigens that correlate with infection. Arrays were probed with a collection of sera consisting of different stages of candidemia: acute, early convalescent (approximately 4 weeks after onset of infection) and mid convalescent (approximately 12 weeks after onset of infection), as well as uninfected hospital patients and healthy individuals. Figure S1 shows a representative image of the microarray hybridized with the serum of an acute candidemia patient. All hybridizations in this study were done under the same conditions and dilutions with protein microarrays printed from the same batch. Their serological reactivity is shown as a heatmap where the antigens are sorted by increasing normalized global mean intensity, with bright green having the weakest intensity, red being the strongest, and black in between ( Figure S2 ). An examination of the IgG response to the entire C. albicans cell surface protein microarray showed that the mean global signal intensity was similar among different groups (data not shown), although antigenic profiles are not identical between individuals.