The DENV consists of an icosahedral ectodomain, containing 180 copies of the E protein [8] . E protein monomer contains three structural and functional domains [9, 10] . E protein domain I (E-DI) is a central b-barrel structure. E protein domain II (E-DII) is organized into two long finger-like structures and contains the flaviviruses conserved fusion loop. E protein domain III (E-DIII) has an immunoglobulin-like fold and may mediate interactions between the virus and the receptors on the host cell [11] . Studies of the biological characteristics and epitope specificities of mouse monoclonal antibodies (mAbs) have elucidated the antigenic structure of flavivirus E proteins [12] [13] [14] [15] . Serotype-specific mAbs with neutralizing activity against DENV-2 have been found to be located on the lateral ridge of E-DIII and the subcomplex-specific mAbs recognized A-strand of E-DIII [14, 16, 17] . Antibody-mediated neutralization has been found to alter the arrangement of viral surface glycoproteins that prevent cells from viral attachment [16] . Binding of an antibody to the viral surface can interfere with virus internalization or membrane fusion [6] .