The final automated processes for the CFTR gene were conducted on a total of 86 samples. The results are reported from two separate processes: (1) PCR through data acquisition (20 samples) and (2) TSE through data acquisition (66 samples; Table 1 ; also see Table 5 in the online Data Supplement for further sample and testing information). Because workstation space is limited on the Tecan Genesis Model 200, tip usage is high when disposable tips are implemented, and amplicon carryover may be a concern. Process 1 may be applied for higher throughput scenarios where the PCR step could be implemented on yet another workstation or manually. For process 1, 20 (100%) samples reported calls correct. For process 2, 64 (97%) samples reported all correct assignments. There were no incorrect determinations.