Although significant progress has been made in epitope prediction through mimotope mapping, we must acknowledge that the performance of any algorithm devised and any tool developed so far was evaluated based on the outcomes of very limited test cases in which the epitopic region must be known and both the structure of the antigen and the peptide set derived from high-throughput screening must be available. If a single method is applied to a case in which the epitopic region is unknown, the mapping simply returns a candidate epitope (or none) with aligned paths formed by the antigen surface residues (or none). Such candidate epitope will become the focus of further investigation through other means.