Single-stranded sense and antisense phosphorothioate oligonucleotides for the predicted GGCGGCGTGCACACACACGC-CCACCGCGG binding site are annealed by boiling sense and antisense oligonucleotides for 5 min at equal molar ratios in dH 2 O. Oligos are then cooled to room temperature and placed on ice. Transfections using DOTAP (Roche)/HEPES solutions are performed with oligonucleotides corresponding to wildtype, mutant or with DOTAP (Roche)/HEPES solution lacking oligonucleotides (MOCK) as described in (29) . Effects of oligonucleotide application to neurons are assessed by real-time RT-PCR.