The laboratory network in Rwanda is contributing to the successful strengthening of the disease surveillance and response system by ensuring involvement of the public health laboratory network in the planning for IDSR, laboratory participation in outbreak investigations, engagement in feedback communications, integration of laboratory indicators to monitor progress, and developing mechanisms for coordination of epidemic preparedness and response at all levels. The coordination and leadership role of the NRL and the commitment of multiple partners under its leadership provide unique opportunities for strengthening the laboratory networking in Rwanda.