The data were analyzed with one-way repeated measures analysis-of-variance (ANOVA) with Tukey post-hoc testing between T = 0 versus T = 30, T = 30 versus T = 60, and T = 60 versus T = 90. P < 0.05 was accepted as being statistically significant. Data are expressed as mean ± standard deviation unless stated otherwise. Statistical analysis was performed using SPSS version 15.0 (Chicago, IL, USA).