One of the reasons for the comparatively low performance on ClustalW alignments might be that those alignments often do not conserve the reading frame. As we wish to make as fair a comparison as possible, we therefore additionally manually adjust alignments to be more 'reasonable' by adjusting gap placement to conserve the reading frame. This does indeed result in considerable inprovement, thus demonstrating the volatility of results when dependent on one particular alignment. However, even when improving the fixed ClustalW alignment, the resulting accuracy after manual adjustment still falls short of that achieved by the statistical alignment method, reaching only 40 -70%.