Briefly, α-MMC was separated on SDS-PAGE and then transferred to an NC membrane. Nonspecific binding was blocked and washed by placing the membrane in a solution of BSA. NC membrane was incubated with a mouse anti-α-MMC McAb and was exposed to a sheep-antimouse Ab linked to alkaline phosphatase. After adding BCIP (5-bromo-4-chloro-3-indolyl phosphate), the colored bands were visualized and photographed. 16 In the analysis of MAP30, NC membrane was incubated with a diluted solution of rat anti-MAP30 PcAb under gentle agitation. Other conditions were the same with α-MMC.