To quantify the mineral resorptive activity of osteoclast-like cells in culture, RAW264.7 cells were suspended in cell culture medium and seeded in a 16-well BD Biosciences Osteologic® Slide (BD Biosciences, MA, USA) at the density of 2×10 3 cells/well and incubated for 24 h. Osteoclast-like cells were successfully generated by dosing with 50 ng/ml RANKL and 25 ng/ml M-CSF every 48 h over the course of 7 days. The wells were incubated with 100 nM of either mAb alone, sCT alone, or mAb-sCT conjugate. Half the volume of the medium and factors were replaced every 48 h. After 7 days of culture, the medium was aspirated, the slide placed in a petri-dish and washed with double-distilled water. To remove the adherent cells, the slide was then soaked in bleach for 10 min and washed with double-distilled water. Slides were air-dried, viewed under an inverted light microscope, and low power images of the calcium phosphate mineral remaining on the slide acquired. To quantify the resorptive effect of osteoclasts, the images were analyzed using the javabased image-processing program ImageJ (NIH, USA).