Here we use the random walk system envisaged in the Nandy plot [11] based on a 2D Cartesian grid where the four bases are assigned to the four cardinal directions: guanine (g) to the positive x-direction, thymine (t) to the negative y-direction, adenine (a) to the negative x-direction and cytosine (c) to the positive y-direction. The method to plot a DNA sequence is to start at the origin and take a step in the positive x-direction for a guanine base, in the negative x-direction for an adenine, positive y-direction for a cytosine and the negative y-direction for a thymine, and proceed likewise for each succeeding base in the sequence, starting each step from the end of the last one taken. This way a succession of bases in the original DNA sequences is represented by a succession of points in the 2D plot, the overall trace being a representation of the distribution of bases in the DNA sequence (see e.g., Fig. 1 human beta globin). The axes essentially represent the excess of guanine over adenine along the x-axis and the excess of cytosine over thymine along the y-axis; thus the plots are basically of instantaneous values of intra-pyrimidine, intra-purine differences as we proceed along the sequence. The end point of such a curve will be given by (N G -N A , N C -N T ), where by N A , N C , N G, N T we mean the total number of adenines, cytosines, guanines and thymines in the sequence being plotted. GC-rich sequences therefore plot mostly in the first quadrant, AT-rich sequences in the third quadrant on this axes system.