Method: A 37-year old male underwent orchiectomy for a testicular mass. Serum tumor markers levels (β-hCG/AFP/ LDH) were normal. Results: Macroscopical examination revealed a 2.1 cm white-yellow tumor. The tumor was composed of the characteristic for seminoma homogenous, clear cells arranged in nests and islands separated by septa and infiltrated by lymphocytes. Immunohistochemically the tumor cells were positive for PLAP, OCT3/4, CD117 and D2-40 and negative for CD30 and CK8.18. There was also a regular pattern of tubular structures infiltrated by the seminoma cells while retaining a low columnar type epithelium. The presence of sphaerical eosinophilic globules (PAS+/dPAS+/AFP−) within the tubular lumina was strongly reminiscent of the hyaline globules of yolk sac tumor. The tubular structures were positive for CK8.18 and negative for AFP, glypican-3 and OCT3/4. Conclusion: The presence of tubular structures with sphaerical eosinophilic globules creates a suspicion for a yolk sac tumor component. The absence of AFP and glypican-3 expression, the regular pattern of the tubular structures and their continuity with rete testis excluded this suspicion. Hyaline eosinophilic globules in rete testis should not be confused with the globules produced by yolk sac tumors.