Full-length genome sequences of CL-1231094, a related clinical strain of enterovirus, and partial sequences of CL-Fnp5 and CL-QJ274218 were obtained as follows. RNA extracted by using the QIAamp Viral RNA Mini kit (QIAGEN) plus DNase treatment or with Trizol was reverse transcribed with random-tagged primer FR26RV-N and amplifi ed with the SMART RACE cDNA Amplification kit (Clontech, Mountain View, CA, USA) with a specifi c forward primer and FR20RV reverse primer (online Technical Appendix 1 Table 1B ) (23) . Amplifi cation products were separated by electrophoresis on agarose gels and fragments (0.6-2.5 kb) were extracted by using the QIAquick Gel Extraction kit (QIAGEN). Purifi ed products were cloned by using the TOPO TA cloning kit (Invitrogen).