Correlation of nonmolecular and molecular subtyping of inherited epidermolysis bullosa K. Veselý * , H. Bucková, L. Fajkusová, M. Veselá, B. Jerábková * St. Anne's Hospital, Dept. of Pathology, Brno, Czech Republic Objective: Inherited epidermolysis bullosa (EB) is a heterogeneous group of hereditary mechanobullous diseases with skin blister formation of variable severity and numerous extracutaneous manifestations. EB comprises three main groups and many subtypes and their recognition is important for prognosis and genetic counselling. Method: We examined skin biopsy samples of 42 patients with EB using transmission electron microscopy, immunofluorescence antingen mapping (AM) and their peripheral blood samples by mutational analysis. Results: In the total number of 42 cases, concordant results of molecular and nonmolecular methods were found in 35 cases (83 %). In 7 patients (17 %) mutational analysis did not confirm the results of ultrastructural and AM analysis. Conclusion: Strong correlation in the results obtained by different methods was observed. Using combination of nonmolecular and molecular diagnostics methods high diagnostic accuracy can be achieved.