Results: Maternal concentrations of all analytes were lower compared to umbilical cord concentrations. Plasma betaine and DMG between mothers and newborns were strongly correlated. Higher umbilical cord choline and betaine were associated with lower birth weight (β=−60 [−89;-31] and β=−65 [−94;-36] ). Odds ratio for LBW was 4.12 [1.15;14.78 ] and 5.68 [1.24;25.91] for the highest umbilical choline and betaine quartile respectively compared to the lowest quartiles. Conclusion: We observed an increased risk of lower birth weight with increased umbilical choline and betaine in venous umbilical cord blood. These results might reflect a change in choline consumption or metabolism or a disturbed placental function.