Intestinal Epithelial Cells. To determine concentration of RV in the stock solution, the method of Reed and Muench [29] was adopted with modifications. Titration results showed that the RV strain had high and variable but not cell-typespecific cytopathic effects on all the cells used ( Figure 2) . Rotavirus, when incubated without T. esculentum extracts on cells, showed dose-dependent cytopathic effect on human small intestine epithelial cells (H4 cells), pig small intestine epithelial cells (CLAB), and bovine calf small intestine epithelial cells (CIEB) (Figure 2 ). For subsequent antiviral testing an RV concentration at and/or below 1 × 10 7.5 /mL TCID 50 was used to avoid cotoxicity with extracts.