The preliminary experiments suggested that the oligonucleotide capture method was capable of detecting one L3 in pools of up to 200 heads or bodies. To further explore the sensitivity of the assay, the experiment was repeated using 10 separate pools containing 200 heads or bodies spiked with a single L3. All pools were found to be positive, suggesting that the oligonucleotide capture assay was capable of consistently detecting a single L3 in pools of up to 200 heads or bodies of S. ochraceum s.l. (Table 2) .