Introduction: HCV is a recognized cause of HCC. Information on HCV genotypes in HCC are scanty in India. Methods: A total of 154 HCC cases from Delhi, 96 HCC cases from Madurai and 246 cases of chronic hepatitis without HCC were controls in the study. RT-PCR for HCV RNA and genotyping were carried out in all the cases Results: In group-I, HCV RNA was positive in 26.58% HCC cases in which genotype 3 was found in 57.1% Genotype 1 was observed in 26.2% HCC cases. Whereas 16.6% cases remained nontypable. In group-II, HCV RNA was positive in 23.95% HCC cases, with genotype 3 in 30.4% cases, Genotype 1 in 52.2% cases and Genotype 4 in 4.34% cases. However, 13.04% cases remained nontypable. Out of the 246 control cases, 187 were CH and 59 were cirrhosis. In CH group, HCV RNA was positive in 22.45% cases in which, genotype 3 was detected in 71.4% cases whereas Genotype 1 was observed in 21.4% cases . However 7.1% cases remained nontypable. In Cirrhosis group, HCV RNA was positive in 37.2%cases. Genotype 3 was found in 59.1% cases. While genotype 1 was present in 31.8% cases and 9.1% cases remained nontypable. Conclusion: Genotype 3 in Delhi and Genotype 1 in Madurai were predominant. in HCC cases. Our study demonstrates that no particular HCV genotypes were associated with HCC and genotype did not appear to influence the development of HCV-associated HCC. 