Because s-RT-MELT does not require size-separation for identification of enzymatically generated fragments, more than one sequence can be scanned in parallel for unknown mutations in a single-tube reaction of Surveyor TM . This simple procedure enables the specificity of the Surveyor TM enzyme to be combined with the throughput and convenience of real-time PCR for rapid mutation scanning. Finally, because the amplified mutated sequences contain defined primers at their ends, direct sequencing of enzymatically selected PCR products is readily possible following the real-time melting step, enabling sequencing of low-level mutations identified by Surveyor TM .