The siRNA screen was performed using the Dharmacon library targeting 21,125 genes HBEC30 were plated into 96 well plates at 10,000 cells per well and siRNAs were reverse transfected. Each siRNA pool was transfected in two sets of triplicates for a total of 6 wells for each siRNA, three wells for infection with IVA and three wells for mock infection, with a concentration of 50 nM for oligos and 0.1% DharmaFECT 3 reagent. Cells were incubated for 48 hrs after transfection and infected with influenza A/WSN/33 (H1N1) virus at an MOI of 5. Forty-eight hours after infection cell viability was assayed using CellTiter-Glo, 15 ml of Promega's CellTiter-Glo was added to wells on a 96 well plate for a final concentration of 7.5%. Plates were rocked for two minutes followed by 10 minutes incubation. Luciferase activity was measured with a PerkinElmer EnVision reader. The miRNA mimic screen was performed with the Dharmacon miRNA mimic library corresponding to 426 human miRNA's. Screening conditions were identical to those described above with the exception of a 72-hour incubation between transfection and infection.