Preparation of substrate for consensus shuffling from 10 non-fluorescent GFPuv clones Ten non-fluorescent GFPuv clones were pooled in equal amounts. The nature and location of the mutations in these clones is shown in Figure 4 . The GFP coding region was PCR amplified from the mixture and submitted to the consensus shuffling protocol with and without the application of the MBP-MutS-H6 error filter.