Protein concentrations were determined using the BCA kit (Pierce). 5 μg total protein was separated on a vertical 12% separating gel. Subsequently, proteins were transferred to PVDF membranes using a Transblot™ Semi-dry transfer cell (Bio-Rad). After blocking for 1 h (0.2% CA blocking reagent, Applichem) immunostaining was performed with primary antibody followed by 4 washing steps (TBS 0,02% Tween 20) and addition of the secondary antibody at appropriate dilution. The blots were developed with chemoluminescent substrate (Supersignal Femto West, Supersignal Pico West, Pierce). The band intensities were quantified using QuantityOne software (Bio-Rad) and ImageJ.