The virus is transmitted by hardwood forest dwelling mosquitoes, Aedes triseriatus, which breed in tree holes and outdoor containers. Ae. triseriatus feed on Eastern chipmunks (Tamias striatus grinseus) and Eastern gray squirrels (Sciurus carolinensis) which serve as amplifying hosts for LACV [7] [8] [9] . Interestingly, the virus can be maintained in the mosquito population in the absence of vertebrate hosts by transovarial (vertical) transmission, thus allowing the virus to over-winter in mosquito eggs [9] . More recently, LACV has been isolated from naturally infected, non-native Aedes albopictus mosquito [10] . The infection of Ae. albopictus may represent a shift in virus ecology and increases the possibility for generation of new reassortants [11] .