Vero-SLAM cells were infected with MeV-Alaska at an MOI of 0.4 pfu/cell in the presence of the inhibitor analyzed ranging from 75 mM to 293 nM in two-fold dilutions. At 96 hours post-infection, cell monolayers were subjected to crystal violet staining (0.1% crystal violet in 20% ethanol), and the absorbance of dried plates at 560 nm determined. Virus-induced cytopathicity was then calculated according to the formula [% rel. CPE= 1002(experimental-minimum)/ (maximum-minimum)*100], with minimum referring to infected, vehicle-treated wells and maximum to mock-infected wells.