Method: For this purpose, we investigated 97 colorectal carcinoma cases, retrospectively. Paraffin embedded blocks was obtained from pathology archive and demographical patients' data from the patient's files of Gastroenterological Surgery Department. Immunohistochemically, we used CD133 and Musashi-1 antibody to determine the stem cells within the tumor. We noted the age of the patients, histopathological diagnose, tumor location, grade, TNM status, clinical stage, disease-free-survival and the outcome. Then we statistically compared all prognostic data with the immunohistochemical findings Results: All of the cases were immunoreactive for both antibodies, furthermore we found a significant statistical correlation (p=0.043) between CD133 expression value and patient outcome. When the value of CD133 expression was high, clinical outcome was poor. In addition, there was a relation between high Musashi-1 expression and poor outcome. Conclusion: Based on these findings we reported that overexpression of both CD133 and Musashi-1 antibodies may be a poor prognostic factor in colorectal cancers.