A high proportion of the wt D b NP 366 + CD8 + T cells in BAL (94.0% (1 0 , d10); 93.2 (2 0 , d8); Fig 2A) and spleen (69.8% (1 0 , d10); 93.7% (2 0 , d8); Fig 2B) bound the D b NPN3A tetramer and produced IFN-c when stimulated by the NPN3A peptide (data not Figure 1 . A stick conformation illustrating how the influenza NP 366-374 interacts with H-2D b . NP 366-374 peptide binds to the H-2D b in an extended conformation. The P3-Asp, P5-Asn and P9-Met are the anchor residues, whereas the P4-Glu, P6-Met, P7-Glu and P8-Thr are solvent exposed and available for contact by the TCR. doi:10.1371/journal.ppat.1001039.g001