Each reaction in the TaqMan assay contained 12.5 mL of Platinum Quantitative PCR SuperMix-UDG (Invitrogen), 90 nM sense (59-CTCATCGTTCTGGCCATCAA -39), 90 nM antisense (59-TCCCATCGAGCCGTTCA -39) primers, 25 nM MGB TaqMan probe (59-CACAACATTATCCGCATC-39), 5 mL of a 1/10 dilution of template DNA in dH 2 0, 0.05 mL of Rox dye, and water to a final volume of 25 mL. The reaction was run on the ABI 7500 Fast Real-Time PCR machine with a thermal profile was as follows: 2 min at 50uC, 10 min at 95uC, 45 cycles of 15 sec at 95uC then 1 min at 60uC. Results were automatically plotted by the Sequence Detection System Software version 1.3.1 (Applied Biosystems).