PCR products were run on an 1.5% agarose gel containing ethidium bromide. Images were captured on E.A.S.Y. RH-3 gel documentation system (Herolab, Germany). Amplicons from the PCR reaction were purified using the MSBH Spin PCRapace kit (Invitek, Germany). Both strands of the amplicons were sequenced with a BigDye Terminator v 3.1 Cycle Sequencing kit on an ABI Genetic Analyzer 3500 6l D6 automated sequencer (Applied Biosystems, Germany) using the corresponding PCR primers. Remaining reaction conditions were performed in accordance to the manufacturer's protocol.