Tissue culture infective dose (TCID 50 ) was determined using the method of Reed and Muench [29] , as previously described in [30] . Clarified supernatants of RV titrating 1 × 10 7.5 TCID 50 /mL (tissue culture infectious dose 50%, TCID 50 ) were subsequently used for the antiviral tests with T. esculentum extracts. Similar TCID 50 values were obtained by Bae et al. [31] (1.27 × 10 6 per mL) when they titrated RV on Macacus Rhesus monkey kidney cells (MA104).