We investigated the neurotropism of APMVs in chicken neuronal cells in vitro and in the brains of 1-day-old chicks infected via the intracerebral route. The neurovirulent APMV-1 strain BC was observed to replicate in chicken neuronal cells both on the basis of antigen expression and the production of infectious virus. In contrast, the non-neurovirulent LaSota strain did not detectably express viral antigens in these cells, and infectious virus was detected in the culture only at a low level on a single day early in infection. Among serotypes 2-9, expression of viral antigen in the neuronal cells was detected only with APMV-3, -7, and -8, and production of infectious virus was detected only with APMV-3. Thus, only APMV-1 strain BC and APMV-3 appeared to replicate productively in these neuronal cell cultures.