In summary, using high-resolution mass spectrometry, we have determined that DENV drastically alters the lipid profile of infected cells. Specifically, DENV infection elevates the expression of lipids that have the capacity to change the physical properties of the bilayer such as bilayer curvature, permeability, and the recruitment and assembly of protein complexes in the membrane. Several of the identified molecules also function as bioactive messengers that control signaling and membrane trafficking pathways in the cells. They represent molecules that result from the activation of cellular stress pathways that respond to viral infections. Based on these findings, the next steps will be to investigate the mechanisms of how these lipid species play a role in DENV replication, as well as identify the control points in these pathways that may be influenced by viral gene products.