PCR detection of PARV4-like DNA using screening primer set A was positive for 2 out of 110 (1.8%) bovine liver samples, while PCR using screening primer set B was positive for 10 out of 14 (71.4%) ovine spleen samples and 6 out of 9 (66.7%) ovine liver samples. The rate of positive PCR detection is significantly higher in ovine liver samples than in bovine live samples (by Fisher's exact test; p,0.001), while no statistically significant difference was noted between ovine liver and ovine spleen samples (by Fisher's exact test; p.0.05). Sequencing of the PCR products was performed: sequences from the two positive bovine liver samples showed the highest nucleotide identities (100% and 89%) to the ORF2 of bovine partetravirus HK2 (GenBank accession no. EU200670), and sequences from the positive ovine samples showed the highest nucleotide identity (88%) to the ORF2 of human partetravirus PARV4 strain BONN-14 (GenBank accession no. EU175857). This suggested that two related but distinct strains of partetravirus are present in the two different bovine samples, while a novel partetravirus is present in the ovine samples.