Generation of mAbs. Identification of antibody variable region genes were done essentially as previously described (Smith et al., 2009; Wardemann et al., 2003; Wrammert et al., 2008) . In brief, single ASCs were sorted into 96-well PCR plates containing RNase inhibitor (Promega). VH and V genes from each cell were amplified by RT-PCR and nested PCR reactions using cocktails of primers specific for both IgG and IgA using primer sets detailed in (Smith et al., 2009 ) and then sequenced. To generate recombinant antibodies, restriction sites were incorporated by PCR with primers to the particular variable and junctional genes. VH or V genes amplified from each single cell were cloned into IgG1 or Ig expression vectors, as previously described (Wardemann et al., 2003; Wrammert et al., 2008; Smith et al., 2009) . Antibody sequences are deposited on GenBank (accession nos. HQ689701-HQ689792 available from GenBank/EMBL/DDBJ). Heavy/light chain plasmids were cotransfected into the 293A cell line for expression and antibodies purified with protein a sepharose. Antibody proteins generated in this study can be provided in limited quantities upon request.