C6/36 cells were infected with DENV at an MOI of 3. Following adsorption, virus was removed and the cells were washed and overlayed with media containing varying concentrations of C75 [51] . The vehicle for C75 was ethanol. Virus supernatants were harvested at 24 hr post-infection and virus titer assayed by plaque assay. Cytotoxicity of C75 was simultaneously assayed using the Quick Cell Proliferation Kit (Abcam). TIME OF ADDITION OF C75: C6/36 cells were infected with DENV as described above. At the indicated time points, 6.3 mM C75 was added to the cells. Cells were harvested at 24 hr post-infection and the amount of released virus was determined by plaque assay. For the lipidomic studies, cells were infected with DENV (as described above) or left uninfected. Following adsorption of the virus at room temperature for 2 hr, C75 was added to the media in the overlay, and cells were incubated at 30uC for 36 hr. Sample preparation, lipid extraction and mass spectrometry analyses were carried out as described above for the fractionated (16K pellet) samples.