Nebulization of genetic material into the lung is eff ective [42] , safe and well tolerated [32, 43, 44] . Th e integrity of AAV vectors [9, 43] and adenoviral virus vectors [44] are maintained post nebulization, as are cationic lipid vectors [32] and DNA and RNA oligonucleotides [45] . A number of gene therapy clinical trials have utilized nebulization to deliver the transgene to the lung [23, 43] , but without clear clinical benefi t to date [43, 44] .