The efficient production of DV1 RSPs by HeLa-prME cell line and the fact that RSPs could mimic the maturation and secretion processes allowed us setting up an assay to study the interaction between DV and host cells during egress, a step which has received little attention in comparison to DV viral entry and replication [40, 41, 42, 43] . We have validated our assay by using a siRNA library preferentially targeting genes involved in cellular transport. As expected, a number of genes involved in the secretory pathway resulted in reduced release of RSPs in the cell supernatant, whereas other factors were associated with an opposite effect. Clearly, the identification of cellular factors involved in the egress process will be helpful to understand the maturation of DV and its pathogenicity. Moreover, our system will allow comparing the effect of cellular factors using RSPs assembled from the four dengue serotypes to test whether there are strain-specific interactions with host proteins.