Phospholipase A2 activity analysis. The lipid samples were reconstituted in 100 mL of methanol : water : formic acid (74:25:1) containing 5 mM ammonium formate and analyzed with an Agilent 6400 QQQ (Agilent Technologies, Santa Clara, CA) coupled to an 1100 Series LC equipped with HPLC Chip interface (Agilent Technologies Santa Clara, CA). Solvent A consisted of methanol : water : formic acid (74 : 25 : 1) containing 5 mM ammonium formate and solvent B methanol : formic acid (99 : 1) containing 5 mM ammonium formate. The gradient was as follows: time 0 minutes, 40% B; time 5 minute, 70% B; time 7 minutes, 100% B; time 9 minutes, 100% B; time 9 minutes, 40% B; time 13 minutes, 40% B. The flow rate was 0.4 mL/min. Data were acquired for parent ions corresponding to the BODIPY-PC (986.9 m/z) and BODIPY LCP (320.2 m/z) in single ion monitoring (SIM) mode with the following source conditions: gas temperature 300uC, gas flow 4 L/minute, capillary voltage 1900 V. The fragmentation voltage was set to 200 V in all cases. Data were processed with Agilent Mass Hunter software version B03.01.