Nuclear protein extraction and electrophoretic mobility shift assay (EMSA) 4 mg of nuclear extracts from Jurkat cells were examined for NF-kB-and Oct1-binding activity by electromobility shift assay (Panomics kit). Samples were resolved on a 6% native polyacrylamide DNA retardation gel in 0.5X TBE buffer and analyzed using a FUJI LA4000 system. 