Gene prediction was performed by Glimmer [39] version 3.02, and results post-processed using TICO [40] for improving predictions of translation initiation sites. Automated annotation of the finished sequence was performed by a modified version of AutoFACT [41] , supplemented by analysis by InterProScan [42] . Manual curation of annotation results was done with support from the software tool GenDB [43] . In addition, annotation of membrane transport proteins was done by performing BLAST search of all predicted genes against the curated TCDB [44] . Ribosomal RNA genes were annotated using the online RNAmmer service [45] . Putative prophage sequences were identified using Prophage Finder [46] . Frameshift errors were predicted using ProFED [47] . CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) were searched by using PILER-CR [48] , CRISPRFinder [49] and CRT (CRISPR recognition tool) [50] .