A glpQ amplicon from another nymphal tick (no. 3) was sequenced (GenBank accession no. EF688578) and was unique in the database; it was also considerably different from the glpQ sequence determined from tick 16, with 325 of 368 bases matching (88.3% identity). The Borrelia glpQ sequence from tick 3 had 85.1%-89.1% identity compared with glpQ sequences from B. hermsii, B. turicatae, and B. parkeri. This fi nding suggests the presence of at least 2 relapsing fever group spirochetes in C. kelleyi that await further characterization.