Statistical comparisons were made using GraphPad Prism software. Results were considered significant when P,0.05. Figure S1 Mice deficient in the inflammasome adaptor ASC (ASC2/2) are partially protected from acute weight loss after intramuscular immunization with rVSV. Average percent initial weight for wild type (n = 10) and ASC2/2 (n = 6) mice after intramuscular challenge with 5610 8 PFU of rVSV. The difference in weight loss between wild type and ASC2/2 mice was significant on the first and second day after challenge (P,0.05, Mann Whitney test).