Different methods have been applied in creating new enzyme such as error-prone PCR [26] , DNA shuffling [27] and staggered extension process (StEP) [28] . Some efforts have been made to modify the enzyme by directed screening but high ratio of negative mutated forms of enzyme in the initial screening is a big hurdle and requires further screening for positive mutated forms of enzyme. Every method has its own advantages and disadvantages that determines the feasibility of a particular method so as sequence truncation [10, 11, 12] . Based on the same hydrolyzing mode, relatively higher thermal stability, and especially the enhanced affinity and catalytic efficiency for GL, deletion of the non-conservative sequence behind the TIM barrel domain was a successful evolution of AtGUS. The truncation of non-conservative region based on sequence alignment could be an effective way of artificial enzyme evolution as it can alter and influence the stability and catalytic efficiency of enzyme, and could help in understanding the relationship between the structural modulation and enzymatic properties.  Protein sequence analysis based on hydropathy profile of amino acids Biology sequence comparison is a fundamental task in computational biology. According to the hydropathy profile of amino acids, a protein sequence is taken as a string with three letters. Three curves of the new protein sequence were defined to describe the protein sequence. A new method to analyze the similarity/dissimilarity of protein sequence was proposed based on the conditional probability of the protein sequence. Finally, the protein sequences of ND6 (NADH dehydrogenase subunit 6) protein of eight species were taken as an example to illustrate the new approach. The results demonstrated that the method is convenient and efficient. The comparative biological sequence is one of the issues in bioinformatics when analyzing similarities of function and properties of different sequences. Similarly, evolutionary homology is analyzed by comparing DNA and protein sequences. In general, there are two types of methodologies to conduct the comparison. One is an alignment-based method, and the other is an alignment-free method.