Finally, a very limited PSCPL analysis was performed for the two chimeric human HLA-A*11:01/porcine SLA-1*0401 MHC-I molecules, HPP and PHP (Table 2) . For both chimeric molecules, it could be demonstrated that position 9 is a strong anchor position. The positively charged amino acids, arginine (R) and lysine (K), were preferred in position 9 of the chimeric HPP molecule, whereas the aromatic amino acid, tyrosine (Y), was exclusively preferred in position 9 of the chimeric PHP molecule. The positively charged amino acids, arginine (R) and lysine (K), were preferred in position 9 of the chimeric HPP molecule similar to the position 9 specificity of the HLA-A*11:01 molecule. In contrast, the aromatic amino acid tyrosine (Y) was preferred in position 9 of the chimeric PHP molecule similar to the position 9 specificity of the SLA-1*0401 molecule.