The sensitivity and specificity of the LAMP assays for the detection of M. ulcerans is shown in tables 1 and 2. Under ambient illumination, positive specimens in the LAMP assay produced greenish colouration (Figure 2 ). When purified DNA extracts were used, 21 (16 swabs, 5 fine needle aspirates) (70%) of 30 clinical specimens were positive by IS2404 PCR as well as by the conventional LAMP. None of the PCR positive specimens were negative by conventional LAMP. However 19 samples of purified DNA extracts were positive with the pwLAMP, but the 90.5% sensitivity (19/21) of the pwLAMP compared to the results by IS2404 PCR was not statistically significant (p = 0.58, Chi-square test).