Reaction. The use of polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origin and it has become an essential tool in the research laboratory [156] . The potential of this format to provide sensitive, specific, and swift detection and quantification of viral RNAs has made it an indispensible tool for state-of-the-art diagnostics of important human and animal viral pathogens [157] . Real-time PCR has engendered wider acceptance of PCR due to its improved rapidity and sensitivity [156] overcoming poor precision, low sensitivity, low resolution, absence of automation, only size-based discrimination, absence of expression of results in numbers, poor quantitative performance (Ethidium bromide for staining is not very quantitative), and post-PCR processing, rendering the conventional PCR not very suitable for accurate diagnosis.