Spleen cells isolated from mice treated with Flt3L/Rapa and other control mice were incubated with anti-CD16/CD32 monoclonal antibody to block FcR, and then stained with PE-cy7-labeled anti-mouse CD11c mAb, Per-cy5.5-labeled antimouse B220 mAb, APC-cy7-labeled anti-CD8a mAb, FITClabeled anti-mouse CCR9 mAb, APC-labeled anti-mouse CD80 mAb and APC-labeled anti-mouse CD40 mAb. CD11c + and CD11c + B220 + gated DCs were analyzed by flow cytometry for expression of the various DC markers. For analysis of regulatory T cells, splenocytes were isolated from the recipient mice and subsequently co-stained with CD4, CD25 or CD3, CD8a CD25 antibodies. The cells were further stained with Foxp3 using established techniques [51] . Cells were then analyzed using FACS flow cytometer as previously reported [52] .