IFN-c responses were detected by both ELISpot and CBA following in vivo boosting with PyCSP DNA (p,0.001 for ELISpot and p,0.05 for CBA) or PyCSP IVTT purified using either NI-NTA resin or MagneHis Ni-particles (p,0.01 for ELISpot), as compared to Alum control ( Figures 4A and 4B ). Responses stimulated by purified IVTT products were almost as robust as those stimulated with PyCSP DNA. Low responses were noted when mice were boosted with unpurified whole extract or unpurified IVTT associated to either Polybeads or ProteinG beads.