By adopting a real-time PCR platform as endpoint detection for s-RT-MELT, the throughput for mutation scanning increases drastically over other mutation prescreening approaches that utilize dHPLC, or capillary and gel electrophoresis. To demonstrate better this point, a highly parallel nano-technology platform was adopted for the real-time PCR step of s-RT-MELT that enables an array of 3072 nl volume real-time PCR reactions (OpenArray TM system) to be carried-out simultaneously followed by differential melting curve analysis (36) . As a proof of principle of the compatibility of s-RT-MELT with OpenArray TM , p53 exon 8 PCR products were generated from 48 different lung adenocarcinoma samples and mutation-containing cell lines and processed via the hybridization and enzymatic steps of s-RT-MELT. The 48 samples were each dispensed in 10 replicate nano-liter volume reactions on OpenArray TM plates pre-fabricated to contain the appropriate primers and amplified in 3072 real-time PCR reactions using a denaturation temperature of 908C in the presence of SYBR-GREEN I dye. Melting curves were subsequently obtained using the OpenArray TM melting curve analysis mode. The PCR growth curves and smoothed differential melting curves obtained distinguish clearly the mutation-containing samples from wild-type samples ( Figure 4C and D, representative results from 3072 reactions). Furthermore, identification of mutation-containing samples is in good agreement between the conventional and the nano-technology platforms ( Figure 4D versus Figure 2H ).