The complete ORF1 is 6855 nt long, and encodes a large precursor polyprotein with an estimated molecular mass of 246.8 kDa. The polyprotein contains characteristic amino acid motifs conserved in caliciviruses: 2C-like NTPase at residue 482 (GPPGIGKT), VPg at residues 958 (KGKTK) and 972 (SEYEE), 3C-like protease at residue 1183 (GDCG), 3D-like RNAdependent RNA polymerase at residues 1520 (GLPSG) and 1568 (YGDD), and VP1 at residue 1867 (PPG). It undergoes proteolytic processing to produce the nonstructural viral proteins and the major capsid protein VP1. Based on comparison with the ORF1 cleavage map of SaV/Mc10 [37, 38] , a human SaV GII strain, we predicted the cleavage site that generates the major capsid protein to be located between residues 1740 (E) and 1741 (G). An in-frame AUG start codon is located in a favourable context for translation initiation (GUGUUUGUGAUGGA) just upstream to the cleavage site, which has also been reported in other caliciviruses [6, 39] . This sequence is noted to be similar to the 59 UTR of the genome, and it was postulated that the site might permit internal translation initiation from subgenomic RNA [39] . The sequence identities of the bat SaV/TLC58 with other SaVs in the complete ORF1 protein sequence vary between 36.0% and 37.4% (Table 3) . While comparison with caliciviruses of other genera, the ORF1 sequence identities are overall lower (15.6%-22.8%) than those between different SaVs (Table S2) . For individual alignment of protease -polymerase, sequence identities with other SaVs (45.3%-48.4%) are overall higher than those with other genera (22.7%-32.1%) ( Table 3 and Table S2 ). The VP1 is predicted to be 546 aa long, and has a molecular mass of 56.6 kDa. It shares 36.1% to 39.2% amino acid identities with VP1 of other SaVs (Table 3) . Likewise analysis with caliciviruses of other genera reveals lower similarities with 14.9% to 23.4% sequence identities (Table S2 ). The complete ORF2 is 615 nt long, with an overlapping region of 8 nt with the 39 terminus of ORF1. Its reading frame is +1 relative to that of ORF1, unlike most other SaVs (Table 2) . ORF2 encodes the minor structural protein VP2, which has an estimated molecular mass of 21 kDa. The mechanism of translation initiation in ORF2 of SaVs has not been fully elucidated. In the present case, a translational upstream ribosome binding site (TURBS) motif (CAUGGGACC; underline indicates region complementary to 18 S ribosomal rRNA sequence) could be identified at 24 nt upstream of the ORF2 start codon. Sequence identities for VP2 with other SaVs vary from 15.5% to 19.9% (Table 3) . By comparison with caliciviruses of other genera, sequence identities are generally lower than those between SaVs. (4.8%-12.3%) (Table S2 ).