Mixed detection combinations of the protease biomarkers are also available that offer benefit in screening applications. In this method, viability is measured first by the addition of the GF-AFC reagent followed by the addition of the luminogenic dead cell reagent chemistry. The use of disparate detection platform methodologies is known to greatly reduce the statistical potential for assay interferences associated with reporter molecules [60] . Therefore this combination of chemistries is favored if a library is uncharacterized with regard to the relative abundance of luminogenic inhibitors or which may cause fluorescence interference. Unlike the fluorescence/fluorescence multiplex, fewer convenient triplex options exist. For example, the chemistry can be configured with a fluorogenic caspase activation chemistry (R110 fluorophore) to deliver data relating to possible induction of apoptosis (Fig. 7A) [61, 62] . Again, negative caspase induction results should be verified by rescreening to verify necrotic response profiles.