Splenocytes were obtained from mice sampled on d6 after secondary infection. Lymphocytes were pre-cultured in the presence or absence of anti-CD8b antibody (53.5-8) (10 mg/ml). Cells were then stimulated for 5 h with peptide, IL-2 and GolgiStop also in the presence or absence of anti-CD8b antibody (5 mg/ml). Following stimulation, cells were analysed for CD8 and IFNc expression. Shown is the percentage of CD8 + cells producing IFN-c after stimulation in the presence of anti-CD8b blocking mAb.