All infections were carried out at a multiplicity of infection (MOI) of 5 plaque forming units (PFU) per cell. Inoculum was removed after one hour of infection at 37uC, cell monolayers rinsed three times with sterile phosphate buffered saline (PBS), and complete media added. Intact and non-adherent cells were collected at six days post-infection, at which time cells and media were collected, counted to determine post-infection viability by trypan blue exclusion, and then centrifuged for 10 minutes at 2086g. Cell pellets were washed twice in sterile PBS and then resuspended in complete RPMI at a concentration of 5610 5 viable cells/mL for continued culture. Cells were counted every three days of culture, and adjusted to a concentration of 5610 5 viable cells/mL. Every six days of culture, cells were counted and centrifuged for 10 minutes at 2086g to remove cell-free cHV68 and cellular debris. Pellets were washed twice in sterile PBS and resuspended in RPMI at a concentration of 5610 5 viable cells/ mL. Culture scheme is depicted in supplement (Fig. S1 ).