At present, according to pathogenicity, antigenicity and nucleotide sequence difference, PCV is divided into two genotypes, PCV genotype I and PCV genotype II, of which PCV genotype II includes various subtypes. From significance of PCV codon usage between different genotypes in Figure 1 , we can see that PCV codon bias may have association with genotypes. In addition to this, the results in this study also reveal that geological factor may almost have relation with codon usage in PCV. In some reports, gene length has certain correlation with codon usage [30] . Similarly, in some viruses, gene length has no effect on codon usage [22] . With correlation analysis we surveyed codon usage bias and gene length in PCV, and it is found that in these viral genes, codon usage bias has no notable correlation with gene length (Spearman, r = 0.075, p > 0.1). The results indicate that PCV gene length has no effect on synonymous codon usage. Other factors, including GRAVY and aromaticity may also significantly influence codon usage of PCV