The inhibitory effect of PEgylated α-MMC and PEgylated MAP30 on HSV-1 TCID 50 was calculated using the method of Reed and Muench, 19 and the emergence of CPE was used as a positive sign. The CPE of each dilution of HSV-1 cytopathic was tabulated as in Table 2 . Measurement of cytotoxicity was designed to establish appropriate dose range for the research of inhibitory effect of proteins on HSV-1 to VERO cells. The result (Figure 9 ) reflected the effects of different concentrations of test proteins on cell viability of VERO cells and showed that α-MMC/MAP30, PEG-α-MMC/MAP30, and ACV had no significant inhibition on VERO cells in the tested concentration range. The cell viability maintained above 97% from 0.003-0.03 µmol ⋅ L −1 and 85% from 0.3-3.3 µmol ⋅ L −1 . Therefore, the dose range of tested proteins inhibiting VERO cell infection with virus was below 3.3 µmol ⋅ L −1 .