Based on these backgrounds, the purpose of this study is to evaluate in vitro gene delivery of AF-liposomes associated with CyDs as a hepatocyte-selective nonviral vector in HepG2 cells. In addition, the mechanisms by which γ-CyD enhanced transfection efficiency of pDNA/AF-liposomes were investigated in the view of a receptor recognition, physicochemical properties (particle size, ζ-potential, and encapsulation ratio), membrane fluidity, cellular uptake, and cytotoxicity of AF-liposomes.