Template set-up within the Luminex LabMap software was required, and importation to analysis software was achieved by dragging the Luminex file folder into the Analysis Software. Data files were parsed, and the resulting raw MFI values were organized by target and sample. After data acquisition from all 225 clinical samples tested, default cutoff windows for each target were empirically determined and set in a blinded fashion. Once determinations were made, reports were generated for offline analysis. The analysis software was written in Java and is compatible with Windows, Mac OS X, and Linux operating systems. Data importation is achievable with Luminex LabMap 100 (software versions 1.7, 2.1, and 2.2) and Bio-Rad BioPlex 3.0. The software displays scatter plots and fraction plots of each given target for all samples.