In this paper, we describe an analysis of termination-reinitiation in the expression of the VP2 protein of murine norovirus (MNV), a calicivirus of the genus Norovirus. The VP2 start codon (AUG) of MNV overlaps the stop codon of the upstream VP1 ORF (UAA) in the pentanucleotide UAAUG, consistent with a terminationreinitiation strategy, and a stretch of bases (59 UAUGGGAA 39) complementary to 18S rRNA helix 26 is present upstream. Using a luciferase-based reporter plasmid, we show that VP2 is expressed by termination-reinitiation and provide evidence consistent with a functional interaction between the coding region of the VP1 mRNA and 18S rRNA. The formation of mRNA secondary structure within the TURBS is also investigated. Overall, our data suggest that the mechanism of VP2 expression is broadly similar to that of the other caliciviruses and influenza B. However, in contrast to what was observed with the FCV signal [13] and seen here with influenza BM2, termination-reinitiation at the MNV signal shows resistance to the initation inhibitor edeine. Thus the mechanism by which the AUG of the downstream ORF is recognised may differ.