For quantification of Ova mRNA, 100 ng of total RNA were reverse transcribed using iScript DNA Synthesis Kit (Biorad). Then, 2 μl of cDNA were subjected to real-time PCR amplification using Ova primers, β-actin-F (5 -AAG ATC TGG CAC CAC ACC TTC T-3 ) and β-actin-R (5 -TTT TCA CGG TTG GCC TTA GG-3 ) primers. For all reaction mixtures, 10 μl of FastStart Universal SYBR green master mix (Roche) was used in a final volume of 20 μl, Ova primers were used at 500 nM and β-actin primers at 400 nM. The same qPCR program as above-described conditions were used. The absolute amount of Ova mRNA for each sample was then normalized against the β-actin mRNA amount (arbitrary units) and determined using serial dilutions of pAAV-CMV-OVA plasmid and β-actin purified PCR product.