Four strains of bacteria and one strain of yeast were used for antimicrobial tests. The test bacteria included Grampositive Staphylococcus aureus (ATCC 6538P) and Bacillus subtilis (ATCC 6633), Gram-negative Pseudomonas aeruginosa (ATCC 9027) and acid-fast Mycobacterium smegmatis (ATCC 14468). Candida albicans (ATCC 2091) was used as a representative of yeast. All microorganisms were purchased in the form of inoculation loops from Oxoid (UK). Nutrient broth with agar and Sabouraud dextrose agar (Acumedia, USA) were used for the cultivation of bacteria and yeast respectively. Mueller Hinton agar (France) was used in antimicrobial screening.