To further investigate the role of dynamin in BTV-1 infection, BHK cells were treated with the general dynamin inhibitor, dynasore [56] . Mock-treated and dynasore-treated cells were incubated with BTV-1, Alexa-568 labelled transferrin or Alexa-568 labelled dextran. Figure 5A -F shows representative cells and that dynasore effectively inhibited uptake of all three ligands. Virtually all of the drug treated cells (n.300 cells per ligand) showed no uptake of virus, transferrin or dextran.