MPSS was developed by Brenner et al in 2000 to address the variabilities, cost and scale of effort required by other high-throughput, genomic profiling methods. It utilizes the technology of DNA megacloning in combination with non-gel-based signature fluorescence sequencing to generate very large numbers of short read-length sequences (9) . This process of megacloning allows the in vitro cloning of fragments of cDNA onto microbeads as opposed to biological hosts. Millions of cDNA fragments can be cloned by hybridization of uniquely tagged molecules to microbeads carrying 10 6 complementary sequences to each oligonucleotide primer used (10) .