Nucleotide sequence homology was observed between the TBFVs and MBFVs particularly in the SL6 region of some JEV group viruses. For example, WNV was observed to share both the stem and loop sequences of TBFV SL6 (Supplementary Figure S1A) . It is of note that the SL6-like region of MBFV maps directly downstream of the highly conserved 5 0 CYCL (Supplementary Figure S1A) .