qualitative parameters which help in the choice of candidate probes that best discriminate between the sequences of the positive and those of the negative set. The general idea of these scores is to distinguish the sequences/groups which are of interest in a given experiment from those who aren't and that can potentially have a cross-contamination with the positive set, because they could be amplified by PCR, contributing to the molecular complexity of the sample. In this article, performances of ORMA were evaluated by considering the experimental evidences coming from the design of LDR probe pairs on two different 16S rRNA datasets. First, a new set of cyano-specific probe pairs was designed and compared to the original one (8), generated on the same database of sequences. Then, the tool was used to setup LDR probe pairs for the identification of pathogenic species present in bovine milk.