In this report we outline the steps we have undertaken to determine whether interference with cane toad tadpole develop- Burst swim speed represents the absolute swim speed normalised to body length, and the full data range (vertical line), standard deviation (box) and mean (horizontal line) are indicated. c: Jumping performance of rAdglob treated and FCA control amimals at stage 46 is shown. Longest jump distance was normalised to body lengths. doi:10.1371/journal.pone.0014576.g003 ment can be achieved using an immunological approach. Our proof of concept approach was largely influenced by previous observations that in bullfrog tadpoles immunised with purified adult globin, the adult globin protein profile was altered in surviving metamorphs [18] . Here we extended the concept to test whether a similar effect could be induced in B. marinus by injecting tadpoles with purified native and recombinant globin as well as viral delivery of this antigen.