Using an open reading frame (ORF) finder (http://www.ncbi. nlm.nih.gov/gorf/gorf.html), three ORF were found in both genomes (Figure 1 ). The ORFs of PBoV1-H18 were 636 aa for NS1, 219 aa for NP1 and 621 aa for VP1/VP2. The ORFs of PBoV2-A6 were 703 aa for NS1, 221 aa for NP1 and 704 aa for VP1/VP2. The possible splicing of bocavirus NS1 transcripts recently shown to extend the length of NS1 proteins was not investigated here [6, 57] . 