One of the primary barriers to the practicality and availability of NA assays in LRS has been the complexity of PCR amplification. PCR is inherently impractical in LRS where reliable electrical power, complex equipment, training, reagent storage, quality programs and clean water, are intermittent or absent. [1, 12] Recently, there have been significant developments in a class of NAATs that do not require temperature cycling. [13] [14] [15] [16] A comprehensive review of these techniques, and their application in LRS has recently been published. [17] These isothermal amplification techniques vary in amplification temperature and duration, as well as complexity of reagents required-and many are proprietary-but all have the potential to be simpler and require less complex equipment than PCR-based assays. These methods use a variety of reaction principles to specifically amplify NA targets through isothermal melting, exponential amplification and intermediate target generation; and which, in several cases, can be detected directly without the need for an instrument. [17] [18] [19] [20] Nevertheless, almost all investigators and manufacturers currently use some type of electrically powered equipment to achieve and maintain the temperature required for amplification, although this equipment can be much simpler than the typical PCR thermocycler. This inherent simplicity makes isothermal amplification more appropriate for diagnostics in LRS.