The NAB response was also monitored from bronchioalveolar lavage fluid 45 days post-vaccination. NAB to ZEBOV-EGFP was undetectable in samples obtained from control (AdlacZ) or I.M. immunized mice, whereas levels of 40610 and 1065 reciprocal dilution were detected in the BAL of nasally and orally vaccinated animals, respectively ( Figure 3 ). Those with pre-existing immunity and immunized by the nasal route had NAB levels of 30610. NAB was not detected in mice with pre-existing immunity to Ad5 and immunized by either the intramuscular or oral route.