Spectra were normalized by total ion current, starting and ending at the M/Z of the collection ranges (1-20 or 20-200 kDa) after baseline subtraction and noise calculation. Outlier spectra were removed. The spectra were aligned to a reference spectrum with the normalization factor nearest 1.0. The spectra were aligned only if the percentage coefficient of variation was reduced after the alignment. Peaks from the different spectra were aligned using the cluster wizard function of the Ciphergen Express 3.0.6 software. The peak detection was automated within the M/Z range of analysis. Peaks were detected on the first pass when the signal-to-noise (S/N) ratio was 7 and the peak was 5 times the valley depth. Peaks below threshold were deleted and all first-pass peaks were preserved. Clusters were created within 0.15% of M/Z for each peak detected in the first pass. The clusters were completed by adding peaks with S/N ratio of 2 and two times the valley depth. P-values and ROC/AUC (Receiver Operating Characteristic/ Area Under Curve) values were calculated by using the P-value wizard.