A band at 150 kDa was seen for unconjugated anti-RANK IgG, with separate bands for heavy chain at 50 kDa and light chain at 25 kDa observed under reducing condition. For mAb-sCT conjugate, a band above 150 kDa was observed, confirming the conjugation to have taken place. Under reducing condition of SDS-PAGE for conjugate, some bands above 50 kDa and 25 kDa were observed, suggestive of conjugation of PEGylated calcitonin to the heavy and light chains of the antibody. (Fig. 2) 