It was reported that immunization with vaccinia virus expressing the entire RSV G glycoprotein results in pulmonary eosinophilia following challenge with live RSV (6, 23, 24) . These studies indicated that RSV G expressed vaccines have a possibility for the development of pulmonary eosinophilia. To determine whether the intranasal immunization of Bac-RSV/G potentiates eosinophilia, the levels of eosinophils in the BAL fluids of the immune mice were examined by flow cytometry 5 days after RSV challenge using antibodies to Siglec-F, CD45, and CD11c as described previously (25) . Bac-RSV/G-immunized mice have a higher frequency of CD11c-SiglecF＋ in the BAL as compared with Bac-control or PBS group (Fig.  7A , p＜0.05), but the level of eosinophil influx in Bac-RSV/G immune mice was relatively weak (∼2% of the total CD45＋ BAL cells). These results suggest that intranasal Bac-RSV/G immunization barely increase the risk of vaccine-induced eosinophilia.