Samples from 8 provinces (24 farms) in Thailand from December 2007 through March 2008 were submitted to the veterinary diagnostic laboratories of Kasetsart University and Chulalongkorn University. A total of 33 porcine samples were confirmed as positive for PEDV by reverse transcription-PCR (RT-PCR) (4) before virus isolation (Table) . Published primers (5) were used for generating the PEDV 651-bp partial S gene. Primers were designed to amplify the PEDV M gene and yielded the amplified product of 715 bp on the basis of CV777 and Br1/87. Products were purified by using a QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany) and were sequenced by 1st BASE Pte Ltd (Singapore).