Optimizing this composition and distribution to facilitate the formation of virus-induced intracellular membrane structures has to be a requirement for efficient DENV replication. Previously we showed that FAS, the rate-limiting enzyme in lipid biosynthesis was both recruited and activated during DENV replication in human cells [20] . In this study we demonstrated that this requirement for FAS activity was also conserved in the mosquito vector-derived cells. Furthermore, inhibition of FAS activity seemed to be most effective between 4-12 hr post-infection suggesting an early requirement for FAS activity during the replication cycle.