Proteins were separated by SDS-PAGE and then transferred onto PVDF membranes using 5% (v/v) transfer buffer, 20% (v/v) Figure 5 . The angiotensin converting enzymes are cell adhesion substrates. Microtitre plates were coated with or without sACE2 and blocked with BSA (1%). Wells were washed; CmF cells plated and allowed to adhere (2.5 h at 37uC). After incubation unadhered cells were washed off and cell adhesion was quantified using MTS reagent and reference to a calibration curve. A) Tabulated cell adhesion results for different patients. B) Graphical representation of (A). C) Cell adhesion is comparable between ACE and ACE2. Plates were coated with sACE2, ACE or PBS and adhesion assays performed as before. doi:10.1371/journal.pone.0034747.g005 methanol. The membrane was saturated with blocking solution (TBS 0.1% (v/v) Tween 20, 2% (w/v) BSA, 5% (w/v) dried milk) (1 h, room temp). Membranes were incubated with primary antibody (4uC, overnight). After washing with TBST four times at 10 min intervals the membranes were incubated with secondary antibody for 1 h at room temperature and then washed as before. Bound antibody was detected using the enhanced chemiluminescence system following the manufacturer's instructions. Densitometric analysis was performed using AIDA software.