By carrying out direct comparisons between the established FQ-PCR method and the conventional PCR method for AHV-1 detection, the results clearly showed that overall the established FQ-PCR detection method is more sensitive and reliable when compared to conventional gel-based PCR, since it was able to detect as few as 1.0 × 10 1 DNA copies of template. Furthermore, this established AHV-1 FQ-PCR method shows more excellent characteristics such as dynamic range (from 1.0 × 10 9 to 1.0 × 10 2 pAHV-1 plasmid copies/μL, which is approximately 10 3 times broader) and sensitivity (detecting pAHV-1 plasmid down to dilutions of 1.0 × 10 1 copies/μL, which is about 2.3 times more sensitive) than other reported method [17] .