Hassl and colleagues (1987) [31] , in a preliminary study, demonstrated some of characteristics of anti-T. gondii IgY and also compared protocols for antibody production and purification, however it was not demonstrated the possible applications of these antibodies to study the parasite. We herein demonstrated for the first time distinct applications for specific IgY antibodies in the T. gondii model. As previously stated, this antibody producing system offers high yield of specific antibodies [32] . We believe that immunization of hens with selected T. gondii targets could improve the range of applications for IgY antibodies. For the P. falciparum model, it has been demonstrated that monospecific and polyclonal IgY antibodies were advantageous [10] . In that sense, immunizations using single antigens of T. gondii could be employed to better understand the role of the selected molecules, as tools to improve the diagnosis of acute toxoplasmosis [33] or the knowledge of hostparasite interaction mechanisms [34] . Additionally, the identification of conserved surface antigens among apicomplexan organisms is usually performed by mammal specific IgG antibodies and contributes to our understanding of parasite evolution [35] [36] [37] .