The collection method and device developed and experiential set up for collecting EBC are shown in Figure 1 and Figure 2 , respectively. As observed in Figure 1 , a simple EBC collection device was developed here. The EBC collection device is composed of four major parts as shown in Figure 1 : collection device cover, collection device base, a layer of ice, and a hydrophobic film (treated by ultralow temperature 270uC). The collection device cover and base were made of Teflon TM polytetrafluoroethylene (PTFE) material, and a parafilm (Parafilm Co. Menasha, WI) used as the hydrophobic surface. The dimensions of the collection device are measured as 80640640 (mm) (length6width6height). In the collection device cover, there is a hole with a diameter of 6 mm as the exhaled breath inlet. The thickness of the collection device cover and base was about 3 mm, and the whole collection device weighs around 105 g. The layer of ice is used to keep the treated hydrophobic film cool.