We termed the extended replicase-like sequences Circovirus-like NI/2007 1-3 (Cvl-NI 1-3), and compared them to a representative set of other replicase sequences (Figure 3) . The Cvl-NI-1 sequence is most closely related to Circovirus-like virus RW-E (gi: 254688530), a circular single-stranded DNA virus previously found in reclaimed water samples in Florida [45] . The Cvl-NI-2 sequence is most closely related to a replicase sequence recovered [48] . The Cvl-NI-3 sequence did not overlap with the other sequences enough to be included in the phylogenetic analysis, but was most similar to Circovirus-like CB-A, a circovirus-like genome identified in a Chesapeake Bay environmental sample (gi: 229562105) [45] . A subset of the positive samples (Table 1) contained sequences from more than one virus, which may be evidence of co-infection. Almost all of the cases with multiple viruses involved TTV-derived sequences along with HHV-6, DENV-2, or circovirus-like sequences (samples 282, 235, 183, 270, 350, and 377). Two samples contained circovirus-like sequences with ASFV-like (sample 315) or GBV-C sequences (sample 387).