Identification of the SL RNA genes from the P. marinus genome project Perkinsus spp. were suspected to possess a SL sequence similar to that of dinoflagellates (DinoSL; [8] ). Two types of SL sequences were detected at the 59 end of P. marinus full-length cDNAs of pcna and cyclins [14] , PmaSL1, 59-ACCGTAGCCATCTTGGCT-CAAG-39 (22 nt) and PmaSL2, 59-ACCGTAGCCATCTGGCT-CAAG-39 (21 nt). These two Perkinsus SL sequences were used to query P. marinus whole-genome shotgun reads [http://www.ncbi. nlm.nih.gov/genomeprj/46451] to identify SL RNA genes. For hits with 85-100% identity to the queries, the genome sequences were collected for alignment with one another and with SL RNAs from dinoflagellates. Type-specific primers were designed for amplifying the putative SL RNAs (Table 1) .