Of various nonviral methods, the lipofection method, by which cationic lipids (cationic liposomes) are used for transfection and interact with plasmid DNA (pDNA) to give a lipoplex, has recently attracted attention [2] . Cationic liposomes have great advantages as gene delivery carriers such as (1) low cytotoxicity and immunogenicity [3] , (2) regulation of the pharmacokinetics through the modification of particle size or lipids components of liposomes [4] , (3) entrapment of pDNA into inner water phase of liposomes and suppression of DNA degradation by DNase [5] , and (4) delivery of gene to target cells by the addition of target ligands and/or antibody [6] .