Complete genome sequences were obtained from the LRT and URT samples of five chronically-infected individuals collected at different time points during the course of infection. Fragments were amplified by PCR using degenerate primers designed to anneal highly-conserved sequences of the corresponding HRV reference strain. Overlapping fragments were assembled and, when necessary, specific non-degenerate primers were designed to fill the gaps between the original PCR products. All primers used in this study are listed in Table S2 . PCR (primers 46 and P1.204) and semi-nested PCR (primers 47 and P1.204, Table S2 ) were performed for VP4/VP2 sequences.