HHV-5 DNA was detected using the commercial real-time test CMV Quant Kit ® (Roche) developed for the LightCycler 2.0 instrument. The test uses internal SCORPIONS™ fluorescent probes for the PCR amplification product. Analogously to HHV-6 detection, for every sample an internal control was added and the amplification was performed in the presence of amplification-specific controls (positive, negative, and extraction process control).