Since the publication of the Centre for Disease Control (CDC) 2001 recommendations for Nucleic Acid Amplification Tests (NAATs) [23] , a multitude of inhouse polymerase chain reaction (PCR) tests have been described, though very few of them have been validated by the CDC. Results of NAATs may be unreliable because of cross-contamination, inappropriate treatment of the clinical samples (leading to the loss of the target nucleic acid) or the presence of inhibiting substances [24] . Additionally, validation of these tests is primarily analytical and not against clinically obtained specimens.