For the anti-GST probing, slides were printed with Sla2p and Myo4p as positive controls and 150 nM BSA as a negative control. The array surface was blocked using SuperBlock (Pierce, Rockford, IL, USA) at 4°C for 1 hour. Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was incubated with the slides at 1,000-fold dilution. The array was then washed with PBST (Sigma) and incubated with a 1:1,000 dilution of Cy5-conjugated anti-rabbit IgG antibody (Jackson Laboratories, Bar Harbor, ME, USA). Slides were then washed with PBST five times and scanned in an Axon