The longest perfectly matching sequence between the mismatches in the InflA probe/21-pm target combination was 3 nt in length. No hybridization was detected at either temperature. In contrast, for the InflA probe/21-gm target combination, where the distribution of the 21 mutations created seven stretches of 5-7 perfectly matching nt between the mismatches, hybridization with the InflA probe was restored (Figure 2A-D) . The MFI signal increased to 20 and 30% (Figure 2A Table 1 sequence of H3N2). The number of dInosines included in the probes is indicated in the names Ino3-Ino21. Similarly, the wobbN_21 probe contains 21 N wobbles in the same position as the dInosines in the Ino21 probe. The complementary target of the InflA probe is named the InflA target. These targets have 0-21 pm, as indicated by the names; the 21-gm target has seven groups of three mismatches ( Figure 2D ). All target molecules were biotinylated at their 5 0 end. Each sample contained 0.2 nM of one of the synthetic biotinylated Continued and 7 and 8% ( Figure 2B and C, 55 C) of the total MFI, when the InflA probe hybridized with the 21-gm target instead of the 21-pm target.