For this report, an assignment is defined as a genotype for a given target within the CFTR gene made for any given sample. Each sample tested will have 30 possible assignments because 30 targets for each sample are analyzed. To rapidly analyze the data obtained, we developed software to present the raw data from the instrument. The software directly imports the Luminex 100 data and organizes them by target mutation. Fractions of wild-type to wild-typeplus-mutant and raw MFI signals can be visualized graphically (Fig. 2) . The program sets default fraction windows at 1.0 -0.8 for wild-type samples, 0.6 -0.4 for heterozygous samples, and 0.0 -0.2 for homozygous mutant samples. Default windows can be changed by the end user. The area between the windows is designated as the no-call window. The program also sets no-amplification windows where wild-type and mutant MFIs are Ͻ200.