Enzyme-linked immunosorbent assays were performed on supernatants of lung homogenates and cell culture supernatants using kits for mouse-specific TNF-␣ (Mouse TNF-␣/TNFSF1A Quantikine ELISA Kit, MTA00); IFN-␥ (Mouse IFN-␥ Quantikine ELISA Kit, MIF00); and IL-10 (Mouse IL-10 Quantikine ELISA Kit, M1000) (all from R&D Systems, Inc.) according to the manufacturer's instructions.