Evaluation of the expression of human and mouse cytokines from different cell types and viral RNA was carried out using iQ SYBR green Supermix (Bio-Rad) according to the manufacturer's instructions. The PCR temperature profile was 95uC for 10 min, followed by 40 cycles of 95uC for 10 s and 60uC for 60 s. Expression levels for individual mRNAs were calculated based on their CT values using two different housekeeping genes (human: rps11 and a-tubulin genes) and (mouse: S18 and b-Actin) to normalize the data.