The calling results are summarized in Table 1 [also see Table 4 in the online Data Supplement for additional details]. The 225 samples included 65 wild-type samples; 93 heterozygous samples representing 22 types; 36 compound heterozygous samples for 9 types; and 31 homozygous mutant samples for 6 types. Of the 225 samples tested manually, 203 (90.2%) samples reported all correct assignments. The failure rate of 10% was most likely a Step 2), tagged target-specific extenders are site-specifically labeled with diGTP-label when the correct amplicon is present. For this study, the label was biotin, and streptavidin-phycoerythrin was used for signaling purposes. ( Step 3), the tags are captured on EraCode-modified Luminex microspheres. The captured reactions are analyzed with the Luminex 100 system. product of DNA quality and not assay sensitivity. As stated in the Materials and Methods, the DNA samples were not fresh, and signal intensities indicated gross failure consistent with PCR failure. There were no discrepancies for genotype classification. For these 225 samples, there were a total of 6750 potential assignments (225 ϫ 27 mutations ϩ 3 reflex tests; 5T7T9T was not included). Correct assignments were made 99.3% (6704 of 6750) of the time. For the separate 5T7T9T-specific reflex test, 194 samples were tested and included at least 10 separate samples from each of the six possibilities (see Table 4 and Fig. 1 in the online Data Supplement). Test results showed that there were zero incorrect determinations and one no amplification failure.