Few or no CD3-positive T cells were present in optic nerves of RSA59-infected mice (Fig. 2D ) and no CD19stained B cells were observed (Fig. 2E ). Spleen sections from mock-infected mice stained with either anti-CD3 (Fig. 2F ) or anti-CD19 antibodies (not shown) served as positive controls. Thus, there was an increase of Iba-1Ypositive cells and few CD3-positive cells in optic nerves of RSA59-infected mice.