The NF-kB responsive genes NFKB1, REL and CCL2, in addition to the JAK/STAT target NOS2A, were used as they had previously been shown to be differentially expressed after inoculation with 1000 particles/cell of DgH HSV-1. Recalling that the more abundant a transcript the fewer number of cycles it takes to cross a threshold set in the exponential phase of PCR product accumulation (the C T value), Figure 2 plots the DC T value on the y-axis, (i.e. the C T value for the gene of interest after normalisation against the RPL13) against the multiplicity of infection on the x-axis. As the multiplicity of DgH increases the C T value decreases, indicating an increase in the abundance of transcripts for all three NF-kB targets (Figure 2(a) ). A logarithmic regression analysis across three logs of multiplicity show a high correlation between the four C T values for each transcript with r 2 .0.98. Similarly for the JAK/STAT responsive target NOS2A there are almost two cycles of a difference between the level of transcripts after infection with 1 particle/cell compared to 1000 particles/cell (Figure 2(b) ). This larger response than those for NF-kB responsive genes is indicative of the different level of induction shown with previous real-time PCR analysis of these transcripts ( Table 2) .