In conclusion, ViralORFeome is the first open-access database that provides an integrated set of bioinformatic tools to build a collection of viral ORFs clones in a versatile system suitable for reverse proteomic experiments. In this perspective, ViralORFeome was especially designed to handle the diversity of virus strains, variants and species. As shown here, our cloning pipeline has been validated using functional assays and a collection of 528 viral ORFs has been generated in the Gateway Õ system. As collaborative efforts between virology laboratories are required to establish an ORFeome collection covering most viral genera and species, we believe ViralORFeome will provide the community with a framework to sustain this global effort.