H5N1 NP peptides from the H5N1-Thailandhuman-2004 strain were predicted by the HLApeptide-binding prediction program, SYFPEITHI 2 . Nine 9-mer potential peptides were synthesized by solid-phase strategies. They were named NP48 KLSD-YEGRL, NP55 RLIQNSITI, NP158 GMDPRMCSL, NP189 MVMELIRMI, NP256 LIFLARSAL, NP275 CLPACVYGL, NP357 QLSTRGVQI, NP373 AMDSNTLEL, and NP458 FQGRGVFEL. A peptide previously identified (N220 LALLLLDRL) was used as a positive control [2] .