Hemolysis assay. The (RXR) 4 À and (RX) 8 ÀPMO conjugates were tested in a hemolysis assay and found to be compatible with red blood cells. Fresh rat red blood cells were treated with the conjugates at 60 mM, PBS (background) or 0.005% TX-100 (positive control). The supernatants of conjugate-and PBS-treated samples had small and similar amounts of free hemoglobin released, far lower than that of the TX-100-treated samples ( Figure 8D ).