The relative abundance of CpG showed a strong deviation from the "normal range" (mean ± SD = 0.622 ± 0.029) and was markedly under represented. Correspondingly, the synonymous codon containing the CpG were inhibited because of CpG was present at the expected frequencies. In detail, low RSCU values were present in 7 of all 8 codon containing CpG and they are all not preferentially used codons (such as CCG, UCG, ACG, CGC, CGG, CGA, CGU) ( Table 1) . These observations indicate that the composition of dinucleotides also plays a key role in the variation found in synonymous codon usage among PCV. 