During the sterilization process, the inlet air blower, inlet air electronic control ball valve, exhaust electronic control ball valve, and extraction blower are all closed. Following this closure, the sterilizing agent by-pass electronic control ball valve is opened. The sterilizing agent in the peracetic acid sterilizer evaporation tank is heated and its vapors are pushed by compressed air into the volume to be sterilized by a sterile connecting hose, a sterilization reagent import, a coupling clamp for the inlet ventilation pipe and an inlet HEPA filter. The exhaust sterilizing vapors escape from the interior of the isolation chamber through an exhaust pre-filter, two-in-series HEPA filters, a sterilizing agent by-pass tube, a sterilizing agent by-pass electronic control ball valve, and an extraction blower to the exhaust export.