Far-UV Circular dichroism (CD) spectra were recorded at 25uC in the range from 190 to 260 nm with a spectral resolution of 0.2 nm using a Jasco J-715 spectropolarimeter. The scan speed was 100 nm/min and the response time was 0.125 s with a bandwidth of 1 nm. Quartz cells with an optical path of 0.1 cm were used. Typically, scans were accumulated and subsequently averaged. The spectra were corrected for the corresponding protein-free control.