Nineteen loci in which putative mutations were identified from the 454 dataset were re-sequenced from PCR products by standard Sanger dye-terminator methods. No false-negatives or false-positives were identified among the re-sequenced loci; however resequencing of the apparent mutation at position 1486408revealed mixed genotypesin several isolates that are artifacts of a large duplication in the 1942 chromosome. Therefore, this signalcannot be considered a true SNP.