We have developed DetectiV, a package for R containing visualization, normalization and significance testing functions for pathogen detection data. DetectiV uses simple and well established visualization and statistical techniques to analyze data from pathogen detection microarrays. DetectiV offers a powerful visualization option in the form of a barplot, enabling researchers to quickly and easily identify possible infectious agents. Data can then normalized to a negative control (be that a specific probe, array or the global median), transformed by taking the log2 and then subjected to a t-test for each species on the array. Oligos are allowed to represent any number of viruses, and thus any analysis is easily extensible by simply updating the list of which oligos represent which species.