This study explored the potential use of the LAMP method for field diagnosis of BU. Some important limitations to the use of this assay in the field include specimen purification and difficulty in maintaining isothermal condition for the reaction. A study suggested that omission of DNA extraction or the use of crude DNA extracts have no effect on the LAMP test [32] . Hatano et al used a disposable pocket warmer to provide isothermal condition for LAMP reaction [34] .