These combinations were further tested in spinner flask on Cytodex1 microcarriers, to confirm their positive effect on Vero cells growth. Data shown in Figure 1 , indicate that cell density level reached 2x10 6 cells/ml after 5 days of culture when Vero cells were grown in combination 1. Such level was slightly lower than that obtained in IPT-AF medium (2x10 6 cells/ml versus 2.4x10 6 cells/ml). However, Vero cell growth in combination 2 was less efficient; the highest cell density Figure 1 Vero cells on 2 g/l Cytodex 1 in spinner flask, in different media obtained in this medium was equal to 1.7x10 6 cells/ml. Thus, combination 1 appears to be more suitable for Vero cells on Cytodex 1 microcarriers.