http://dx.doi.org/10.3343/alm.2012.32. 1.44 www.annlabmed.org These limitations can be overcome by the use of multiplex PCR methods, which enable the rapid and accurate identification of up to 9 bacterial and viral pathogens in clinical samples [8] [9] [10] . We evaluated the diagnostic value of the newly developed Seeplex Meningitis ACE Detection kit (Seegene Inc., Seoul, Korea), a multiplex PCR kit that uses dual priming oligonucleotide (DPO) methods. This kit detects the 12 most common bacterial and viral pathogens of acute meningitis, namely, Streptococcus pneumoniae (SP), Haemophilus influenzae (HI), Neisseria meningitidis (NM), Group B streptococci (GBS), Listeria monocytogenes (LM), herpes simplex virus (HSV)-1 and HSV-2, Varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus 6 (HHV-6), and human enterovirus (HEV).