The following reagents were used for the proteomics sample analysis: nanopure or Milli-Q quality water (~18 megohm cm or better); Amicon Ultra centrifugal filtration tubes were obtained from Millipore (Bedford, MA, USA) ammonium bicarbonate, ammonium formate, and formic acid were obtained from Fluka (St. Louis, MO, USA); Tris-HCl, urea, thiourea, DTT, iodoacetamide, calcium chloride, and TFA were obtained from Sigma-Aldrich (St. Louis, MO, USA); HPLC-grade methanol (MeOH) and HPLC-grade ACN were purchased from Fisher Scientific (Fair Lawn, NJ, USA); 2,2,2-trifluoroethanol was obtained from Aldrich Chemical (Milwaukee, WI, USA); and sequencing grade-modified trypsin was purchased from Promega (Madison, WI, USA). Sodium tetraborate, glycine, and picrylsuofonic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA).