The FAS inhibitor, C75 is a potent inhibitor of DENV replication in both mosquito ( Figure 1 ) and mammalian cells [20] . Cells treated with non-cytotoxic concentrations of C75 (,50 mM) induce an environment that has redistributed its lipid repertoire to support cell survival in the absence of FAS activity, and yet, this environment does not support viral replication. To determine the basis for this exclusion of virus replication, we profiled the lipidome of cells treated with 25 mM C75. Membrane fractions isolated from DENV infected and mock control cells in the presence or absence of C75 were profiled. A hierarchical clustering analysis of the lipidomic data ( Figure S3 ) indicated that the lipid environment of DENV infected cells treated with C75 clustered very closely to the mock C75 treated control and was furthest from DENV infected cells suggesting that the two represent significantly different environments. A comparative analysis of the lipid species expressed in the two environments indicated that a majority of the lipids that were up regulated in DENV infected cells were down regulated upon treatment of those cells with C75.