Interestingly, even the Ino21 probe hybridized quite strongly with the InflA target, at 26 and 39% (Figure 2A and C, 45 C) and 11 and 13% ( Figure 2B and C, 55 C) of the total MFI. Importantly, the Ino21 probe was able to restore hybridization with the 21 pm target (11 and 37% of total MFI) to which the InflA probe had totally failed to bind (Figure 2A and C, 45 C). The Ino21 probe hybridized with all the matching 3-21 pm targets with almost the same efficiency (mean 29.5% of the total MFI, 1946 MFI, at 45 C, Figure 2A; and 8% of the total MFI, 435 MFI, at 55 C, Figure 2B ), which is in the same range as the hybridization of the InflA probe with the 21-gm target (30.4% of the total MFI, 2005 MFI, 45 C, Figure 2A ; and 7.8% of the total MFI, 444 MFI, 55 C, Figure 2B ). The Ino21 probe and 21 gm target combination had 13 mismatches outside the dInosine positions and failed to hybridize (Figure 2A , B and C). In conclusion, the presence of dInosine in the probe decreased hybridization with a perfectly matching target, but a dInosine-containing probe bound more strongly than a dInosine-free probe when the target had many mismatches juxtaposed to the dInosine residues.