It has been previously reported that de novo lipid biosynthesis was up regulated during DENV infection [20] . Consistent with these observations, several primary fatty acids (palmitic, stearic and oleic acid) involved in the biosynthesis of higher order PLs were detected in our lipidomic analyses. Palmitic acid is the first fatty acid of the lipogenesis pathway and stearic acid (C18:0), is immediately downstream of palmitic acid. They are both up regulated in DENV infected cells. The latter is the precursor to the biosynthesis of oleic acid (C18:1) or long chain fatty acids (with acyl chains of C20 or greater). Interestingly, since oleic acid is down regulated in DENV-infected cells stearic acid may be stimulating long chain FA biosynthesis. However, a majority of the PLs expressed in DENV infected cells have C16-or C18-acyl chains with varying degrees of unsaturation. Therefore, it seems that desaturation of palmitic and stearic acid rather than elongation may be the chosen pathway for lipogenesis during DENV infection.