For analysis of IgG1 binding to F1-or V-antigen peptides, the peptides were added to the plates in 0.05 ml per well at 25 mg/ml. and the plates incubated overnight at 4uC in Immunlon 2HB 96well plates. After the washing and blocking steps as described above, binding of the human and mouse mAbs IgGs was detected as described above. The binding of all mAbs to the peptides was evaluated at 10 mg/ml.