Apart from diamidines, polyamines and guanidine also have the potential to give antitrypanosomal compounds. The success of DMFO as polyamine inhibitors has attracted researchers for synthesis and development of agents targeting polyamine metabolism. Recently polyamines carrying 1,3,5-triazine units have been synthesized and evaluated against T. brucei. These compound exhibited good activity but are cytotoxic. Morpholine and dihydroquinolines have also shown promising in vivo anti-trypanosomal activity. In addition to this development of prodrugs, the existing compounds also promise to address the pharmacokinetic related problems, in near future.