To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. This modified technique was originally developed to detect distinct/genetic alterations in large regions of human genome. Afterwards, mPCR was used for the detection and differentiation of multiple pathogens/different strains of the same pathogen. It has additional advantages such as costeffectiveness and rapidity. There are reports on the use of mPCR for differential diagnosis of FMDV serotypes [27, 36] . One such PCR [36] was evaluated in WRL FMD Pirbright and found to work on tissue samples with limited success [148] . To improve the overall diagnostic sensitivity and to provide tools for serotyping FMDV, multiplex PCR arrays have been developed to detect FMDV in infected tissue and food samples [150] .