Quantification of relatively gene expression in human hepatoma Figure 6 Quantification of relatively gene expression in human hepatoma. A total of nine differentially expressed genes including six (GBA, PTPRF, GNL3, ERBB3, OGDHL, FMO3 belonging to Class III) identified only by the SSH/microarray assays and three (C9, MT1F, MT1X belonging to class II) identified by both of the SSH/microarray and regular cDNA microarray assays were assayed for their relative expression between HCC and the corresponding non-HCC liver tissues of 18 patients of hepatoma using real-time semi-quantitative RT-PCR. The results are presented with the log 2 values of the gene expression ratio's between HCC vs. the matched non-HCC liver tissues. Left panel is the relatively expression level of these genes initially determined by the SSH/microarray and regular cDNA microarray assays, respectively. Of note, the difference of the expression for the six genes belonging to Class III was originally only identified by the SSH/microarray assays, but not by the regular cDNA microarray assays. the spot and background intensities with the GenePix Pro 4.1 software (Axon Instruments, Inc., CA, USA). The within-slide normalization was done using programs written with MATLAB 6.5 software (The MathWorks, Inc., MA, USA).