In this study, the impact of adaptation and harvesting time point on HA N-glycosylation of IVA-Uruguay was investigated. So far, it is not clear whether differences in the HA N-glycosylation have an impact on immunogenicity or other properties of influenza vaccines. Other factors, e.g. differences in cell culture media, cell density, etc. may also contribute to variations in HA N-glycosylation. Nevertheless, monitoring N-glycosylation patterns during vaccine production processes allows not only to evaluate antigen quality and the impact of process modifications on lot-to-lot consistency but also to critically assess consequences of unwanted process variations or process failure.  Isolation of active peptides from plant hydrolysates that promote Vero cells growth in stirred cultures  Background Vero cells are adherent cell lines commonly used for the production of viral vaccines. We had developed an animal component free medium that allows an optimal growth of this cell line in stirred bioreactor [1, 2] . We had also showed that Vero cells grown in this medium (called IPT-AFM) sustained rabies virus replication, and resulted in an overall yield comparable to the level obtained in serum-supplemented medium.