Analytical HPLC HNP1 preparations derived by enzymatic or CNBr treatment were analyzed on an Alliance 2690 Separations Module using a C18 column (3 mm particle, 180 Å pore, 2.0 mm6150 mm, Varian Polaris 5A) with a flow rate of 0.2 ml/min using a 0-to-60% ACN linear gradient in 0.1% aqueous TFA at 1% per minute. Eluting peaks were detected at 210 nm and 280 nm using a Waters 2487 Dual l absorbance detector using Clarity software. Natural (human neutrophil-derived) HNP1 was used as standard in these analyses.