Objective: ETS gene rearrangements are frequently found in prostate cancer (PCa). Recently, we observed that ERG rearrangement in primary PCa transfers into lymph node metastases, suggesting it to be a clonal expansion event during PCa progression. Here, we investigated whether this also applies to the less frequent ETS genes, ETV-1, ETV-4, ETV-5 and ELK-4. Method: Using break-apart FISH assays, we evaluated the status of above mentioned ETS gene rearrangements on a cohort comprising of primary PCa, corresponding lymph node and distant metastases. Results: ETV-1, ETV-4, ETV-5 and ELK-4 rearrangements were found in 10 %, 6 %, 1 % and 2 % of the primary PCa, respectively, and in 8 %, 6 %, 6 %, 1 % of the corresponding lymph node metastases, respectively. Rearrangements of ETV-1 and ETV-5 were not found in any of the distant metastases cases, whereas ETV-4 and ELK-4 rearrangements were found in 4 % and 4 % of the distant metastases, respectively. Conclusion: Our results suggest that rearrangement of the less frequent ETS genes is a clonal event during prostate cancer progression. Our findings provide insights into potential clonal expansion events during PCa progression and may have significant implications in understanding the molecular basis of the metastatic cascade of PCa.