C. albicans cell surface protein microarray construction and hybridization C. albicans cell surface proteins were chosen for the protein microarray because they interact directly with the host and thus are likely important for colonization and infection, as well as likely targets for the host immune system. Furthermore, many of their protein expression levels are regulated in response to extracelluar signals, such as stress, nutrients, host factors, or changes in environment. Known antigenic proteins are also included as controls (Bgl2, Eno1, Pgk1, Gap1, Cdc19, Tkl1, Hsp90, and members of the Hsp70 family) [15, 17] . The collection contains 451 His-and HA-tagged peptides (Table S2 ) that represent 363 different proteins, since ORFs .3,000 bps were cloned into two or more segments. All tagged proteins were confirmed individually by western blot and again on the protein microarray.