In a parallel experiment, animals were treated with 1.0 · 10 6 PFU of rAd5 and challenged 36 h later with 50 ID 50 of HKN/2002. To test whether the combined inoculations of rAd5 and rS. cho protected animals, some groups were injected intramuscularly with the p3D-NT56/S. cho+Ad5-POL mixture of 1.0 · 10 9 CFU and 1.0 · 10 6 PFU, respectively; then, after 36 h, they were challenged with 50 ID 50 of HKN/2002. 