probe as described previously (Tsurui et al., 1994 ; Wakita The translation efficiencies of the variants with a muta et al., 1994) . A single band for each of the aminoacyltion at position 33 were also examined in a cell-free tRNAs was detected by staining ( Figure 4A ) with which translation system of C.cylindracea (Yokogawa et al., the radioactivity coincided in each case ( Figure 4B ). 1992; Suzuki et al., 1994) , to evaluate the effect of G33.