For all viral antigens, robust IFN-c responses were detected by CBA in cultures stimulated at 1:100 dilution with IVTT products purified using NI-NTA resin ( Figure 7A ) or MagneHis Ni-particles ( Figure 7B ) but not with purified products at 1:1,000 or 1:10,000 dilutions, presumably due to the low amount of antigen in culture ( Figures 7A and 7B ). With unpurified IVTT products or IVTT antigens associated to Polybeads or ProteinG beads, IFN-c responses were generally better at the higher dilution (1:10,000.1:1,000.1:100) where background E. coli responses were lower (Figures 7C-E) . However, none of these IFN-c responses were statistically different from the controls due to variations in the cytokine levels between individuals (p,0.05 only for EBNA3A-Protein G beads at 1:1000).