Quantitative PCR assays (qPCR) were established based on CedPV-specific sequences obtained from the high throughput sequencing. A TaqMan assay on the P gene was developed and used for all subsequent studies. The sequences of the primer/probe are as follows: forward primer, 59-TGCAT TGAGC GAACC CATAT AC; reverse primer, 59-GCACG CTTCT TGACA GAGTT GT; probe, 59-TCCCG AGAAA CCCTC TGTGT TTGA-MGB.