HBoV DNA was detected in NPA samples from 1 (0.8%) of 126 symptomatic adults (71 years of age) and from 31 (13.8%) of 225 symptomatic children (mean age 17 months, median age 15 months). However, HBoV was detected more frequently (43%, p<0.001) in the 100 asymptomatic control children (mean age 22 months, median age 23 months). Another virus was detected in 22 (71%) of 31 HBoV-positive NPAs from symptomatic children. The virus most commonly co-isolated with HBoV was hRSV (16/31, 52%), followed by infl uenza A/B (3 cases), hMPV (3 cases), adenovirus (1 case), and parainfl uenza virus (1 case). Two children were infected with 2 other viruses in addition to HBoV. The median age of symptomatic children with HBoV infection (15 months) was signifi cantly greater than that of symptomatic children without HBoV infection (8 months; p<0.0001). The hospital length of stay was similar for children positive for HBoV DNA (mean 5.1 days, median 4 days) and those negative for HBoV DNA (mean 6.6 days, median 3 days) (p = 0.9).