Loop-mediated isothermal amplification (LAMP) is one of the DNA amplification technologies that employ a constant temperature [64] . The Bst polymerase plays a key role in the LAMP reaction process. The Bst polymerase, which is derived from Bacillus stearothermophilus living in hot springs with temperature around 70 °C, has polymerize activity, 5'-3' exonuclease activity, and strand displacement ability. At a suitable temperature, Bst polymerase with strand displacement activity can separate the non-template strand from the template DNA without the thermal cycles of the PCR process, which uses Taq polymerase to synthesize new DNA strands. Subtle primer design is also necessary for a successful LAMP reaction. In the first stage of the reaction, the so-called outer and inner primer pairs can make dumbbell-like loop DNA strands from the target DNA templates, and the dumbbell-like DNA strands become the new template DNA for the next step ( Figure 4) . The dumbbell-like DNA strands then continue replicating to become a flower-like long-chain DNA product [65] . In addition to these two primer pairs, a third pair known as loop primers has been designed and proven to be beneficial in accelerating the amplification process. A good primer design not only ensures successful execution of LAMP, but also increases the sensitivity and specificity of the reaction result [66] . Thus, the LAMP reaction is carried out by three pairs of primers in an isothermal condition. Compared to the PCR, the reaction time of LAMP is shorter while the sensitivity and specificity are almost the same or even better. For fixed temperature heating, the heater component of the device can be simpler relative to traditional DNA amplification instruments. These features afford LAMP strong potential as a disease screening method based on the economic benefits of clinical point-of-care devices with simpler designs. Because of convenience, high efficiency, and the specificity of LAMP, it has been applied to many DNA screening tests, especially virus detection.