The airflow direction through the isolation chamber via the air inlet and outlet is shown in Fig. 1 . Room air is drawn into the interior of the isolation chamber through the inlet pre-filter, inlet air blower, inlet ventilation pipe, inlet air electronic control ball valve, and inlet HEPA filter in the animal breeding mode. The air from the isolation chamber is drawn out of the exhaust export through the exhaust pre-filter, exhaust HEPA filters A and B arranged in series, exhaust ventilation pipe, and exhaust electronic control ball valve via an extraction blower. The HEPA filters are arranged in series to ensure that if one fails, the other can still ensure exhaust security and prevent pathogens from being discharged into the atmosphere.