To further test the status of viral replication in endothelial cells, we infected MB114 endothelial cells with GFP-cHV68 and collected the intact, non-adherent cells at six days post-infection. GFP expression is driven by the CMV immediate early promoter and has been demonstrated to be expressed only during lytic infection [29] . At the time of harvest, these cells uniformly expressed GFP (Fig. 3D histogram) and continued to do so at 20 days post-infection, (Fig. 3D micrographs) . Therefore, the nonadherent endothelial cells which remained intact after cHV68 infection did not escape infection and were not latently infected.