JV V5 and JV FLC T7 cDNA plasmid constructs were linearised using NdeI (Invitrogen). Capped RNA was synthesised using the mMessage mMachineH Capped RNA Transcription kit (Ambion) according to the manufacturer's instructions. CRFK cells were seeded into 6 well trays at approximately 50% confluence and were transfected with 2 mg purified RNA per well using Transmessenger transfection reagent (Qiagen) according to the manufacturer's instructions.