Isolated splenocytes were added to U-bottomed 96-well plates (Corning Inc) at 10 6 cells/well in 100 μL complete RPMI-1640 medium supplemented with 10% FCS. The cells were then mixed with 100 μl media containing Note: The 27 peptides with highest binding score (BS) as predicted by each algorithm are shown, starting with the peptide giving the highest score at the top for each protein. Sequences of 9 mers are given from BIMAS, SYFPEITHI and PRED BALB/c predictions, respectively. The N-terminal amino acid position is indicated for epitopes predicted from the whole M protein.