Sequence analysis of the HBoV VP1/VP2 genes performed on ≈50% of HBoV-positive specimens showed 2 distinct clades of viruses (Figure) . These genotypes clustered with the original strains described by Allander et al. (ST1, GenBank accession no. DQ000495, and ST2, Gen-Bank accession no. DQ000496) (1). There was no temporal link between the clades because both were equally distributed throughout the study period. No obvious relationship was found between clades and the presence or absence of symptoms.