In this study, we identified three types of astroviruses, TAstV-1-like, TAstV-2-like and ANV-like, circulating in turkey flocks in Poland. The most frequently identified were TAstV-2, with TAstV-1 occurring rarely and ANV occasionally. Isolated astroviruses were found to be genetically variable based on the sequence analysis reported here. This diversification was particularly noticeable among Polish TAstV-2 strains, as some of them were genetically related to the North American isolates. However, most of them formed a distinct subgroup of ''European'' isolates, suggesting their separate origin or evolution. In spite of the fact that some isolates were from healthy flocks, it could not be ruled out that astroviruses caused the observed health problems and poor performance of meat-type turkey flocks in Poland. It should be also mentioned that in the light of the new classification rules, none of the Polish turkey astroviruses studied here could be assigned to any of the genotypes. In order to enable classification of these viruses, a phylogenetic analysis of the full-length ORF2 gene is needed, and according to the new taxonomy proposal, they are classified as '' related viruses that might be members of the genus Avastrovirus but have not been assigned to a species'' [23] .