The tail of the HBoV1 head-to-tail junction [28, 29] was found to contain a sequence (59- GCG CCT TAG TTA TAT ATA ACA T -39) identical to that of the right-end hairpin (REH) of the other prototypic bocavirus MVC [27] . We thus speculated that the entire HBoV1 REH is similar in structure to its MVC counterpart. Using a reverse primer targeted to this sequence (RHBoV1_nt5464) and a forward primer located upstream of the REH (FHBoV1_nt5201), we were able to amplify a specific ,300-bp-long DNA fragment ( Figure 1D , lane 5). Sequencing confirmed the presence of the palindromic hairpin of the predicted REH (nucleotides in red; Figures 1C and S1C), and revealed two novel nucleotides at the end of the hairpin (GC in red; Figure 1C ).