The results of effective information of mammals are also consistent to the recently published article regarding the amount of constrained sequence in genome shared between eutherian mammals [26] . The constrained sequence means the sequence under functional constraint. The total amount of constrained sequence in rodents is estimated as 260Mb (5.2e8 bits), which is close to 5.09e8~6.34e8 bits effective information of rat and mouse. 300Mb (6.0e8 bits) of human genome is estimated under functional constraint. This is also close to 8.38e8 bits effective information of human. For fruit fly, the amount of constrained sequence is estimated as 55.5~66.2Mb (1.1~1.32e8 bits), which is also close to 1.09e8 bits effective information. Therefore, the effective information can be used as an estimate of functional fraction of genome.