Statistical analysis of processed data. Following chromatographic alignment and database matching, the abundances of all detected features (both AMT tag database matched and unmatched) were loaded into DAnTE [60] for statistical analysis. Feature abundances were transformed to log2 scale then subjected to central tendency normalization [61] . Comparative data analysis was then performed and statistically significant differences between the lipid profiles of the samples were determined using ANOVA. Partial least-squares (PLS) [62] analysis was also performed using the data matrices containing either AMT tag database matched features alone or all features (both database matched and unmatched). homogenized and centrifuged at 15006 g, at 4uC for 5 min to remove nuclei. Post-nuclear supernatants were centrifuged at 160006g for 30 min at 4uC to obtain a membrane pellet (referred to as the 16K pellet). The supernatant post nuclear and membrane fractionation is referred to as the cytoplasmic extract (CE). Total RNA was extracted from each fraction (16K and CE) using Trizol reagent (Sigma) according to the manufacturer's instructions. Following RNA extraction, total lipids were extracted using the Bligh and Dyer method mentioned above and counted using a Beckman LS 6000 scintillation counter. The viral RNA in each fraction was measured using the SuperScript III Platinum SYBR Green One-Step qPCR Kit with ROX and DENV specific primers; (forward) 59 ACAAGTCGAACAACCTGGTCCAT 39 and (reverse) 59 GCCGCACCATTGGTCTTCTC 39 on a Applied Biosystems 7300 Real Time PCR machine. The labeled lipid in subcellular fractions was standardized to the total RNA isolated from each fraction prior to determining the viral RNA genome copies per labeled lipid in subcellular fractions.