Importantly, when dInosines matching the positions of the sm in the targets were included in the probes, the Ino18 probe hybridized with all the sm-containing targets, although with slightly varying MFI ( Figure 3A , B and confirmed in C). The 33%9F target, which did not hybridize with the InflA probe, was able to hybridize with the Ino18 probe (66% of the total MFI, 3894 MFI at 45 C, Figure 3A ; 55% of the total MFI, 2808 MFI at 55 C, Figure 3B ). Even the 33%9-15nt targets, with two mismatching nt at the 3 0 end not covered by dInosines, hybridized well with the Ino18 probe (48-51% of the total MFI, 2818-2994 MFI at 45 C, Figure 3A ; and 16-32% of the total MFI, 778-1463 MFI at 55 C, Figure 3B) . Thus, as shown in the previous series of pm matched to dInosine, when the sequence is interrupted too frequently by mismatches, leaving no suitable regions of perfect match, a probe with dInosines at the positions of variation will restore the hybridization by effectively creating the required longer matching region.