Lateral flow test strips provide a promising tool for the development of point-of-care nucleic acid biosensors. Consequently, HDA has been employed with an embedded lateral-flow DNA detection strip for end-point assay to detect HIV-1 in human plasma [84] . The principle of this approach is based on a sandwich immunoassay using two probes: a fluorescein isothiocyanate (FITC)-labeled capture probe and a biotin-labeled detection probe. The HDA products hybridize with the capture probes and detection probes to form the complex. The hybrids are bound to streptavidin-conjugated color particles and are captured on the test zone by the interaction between the target DNA-FITC capture probe and an anti-FITC antibody. The accumulation of color beads in the test zone of the fiberglass paper is visualized as a characteristic red band. This assay provides the satisfactory detection of HIV-1 RNA at 50 copies/assay. This disposable amplicon detection device based on HDA has also been applied to the herpes simplex virus [85] and Mycobacterium tuberculosis diagnosis [86] and shows a performance comparable with conventional detection assay. Nevertheless, sample preparation, target amplification, and nucleic acid testing are conducted as distinct steps. 