A grown bacterium has roughly fixed volume, mass, and the number of protein molecules. It needs to produce all the protein molecules in its growth. The production of proteins in the growth is like chain reactions controlled by complex regulatory cascades with feedbacks. Even though the production of proteins is complex, we can always think that a bacterium grows theoretically from producing the first protein molecule to the last molecule in sequence. In this way, we can calculate the effective information. The problem is that many molecules may be produced simultaneously in the process. This may need less information. However, like viruses, in order to change protein expressions flexibly, organisms may use an information mechanism that can produce protein molecules one by one in sequence to reduce the maximum entropy to 0, which means each protein or operon has at least one regulatory sequence. Even if the calculated information may be more than necessary, we can still use k value later to adjust the information to fit to the actual amount of effective information based on real regulatory information of organisms.