Based on the total number of lipids that were significantly regulated between groups (uninfected and infected, Anova p,0.05) and subsequently structurally identified (Table S1 and Methods) the overall abundance of lipid classes is as follows: phosphotidylcholine (PC), 39.2%; phosphatidylethanolamine (PE), 31.2%; phosphatidylserine (PS), 0.8%; sphingomyelins (SM), 18.5%; ceramide (CER), 5.3%; lysophospholipids, 0.8% and ceramide phosphoethanolamine (CER-PE), 4%. This distribution is similar to the membrane lipid composition of eukaryotic cells where the most abundant phospholipid is PC [21] . It is also consistent with the membrane lipid composition of the Diptera species where PE is a predominant PL [22] . CER-PE, which is preferentially expressed in insect cells, is also observed here [23] [24] . Several of these lipids are differentially regulated upon exposure of cells to DENV or UV-DENV ( Figure 3 ). It was noticeable however, that several negatively charged lipid classes such as phosphatidylglycerol (PG), phosphatidylinositol (PI), and Cardiolipin, were absent from this list. These lipids have previously been reported to account for 6-13% of the lipidome of mosquito cells [25, 26] . However, in this whole cell analysis, these lipids were not significantly regulated during virus infection.