We show herein that the RV strain used (RF strain) could be adapted to pig and human cells, additional to bovine cells. T. esculentum bean cotyledons had low or no cytotoxicity, as shown by high IC 50 concentrations ( Table 2; Figures 3  and 6) , while seed coat and tuber extracts, especially seed coat ethanolic extract (MSCE) and tuber water extract (MTW), had elevated cytotoxic effects (low IC 50 concentrations lying between 0.01 and 0.001 mg/mL) on all the cells used (Table 2 ; Figures 3 and 6) . This study has identified T. esculentum bean ethanolic cotyledon extract (MCE) and bean seed coat extract (MSCE) as having highly significant inhibitory effects against cytopathic effects of RV, both with coincubation ( Figure 3 ) and preincubation ( Figure 6 ) of the extracts and RV on human, pig, and bovine intestinal epithelial cells. Tylosema esculentum seed coat ethanolic extract (MSCE) showed the best antirotaviral activity, as shown by the lowest IC 50 concentrations (1 μg/mL).