The endo IV reaction was prepared by adding 5 ml Endo IV Master Mixture to 5 ml PCR mixture. Reaction was incubated at 50 C for 50-60 min. Fluorescent detection was performed on ABI 7700 or 7900 in the two channels typically used to detect FAM (for FL1) and VIC (for FL2, FL3 and FL4) utilizing the ABI software for Allelic Discrimination. Alternatively, the fluorescence could also be detected in a Figure 3B and D, respectively. The reaction mixture contained Endo IV at 0.04 U/ml concentration, probe and enhancer at 150 nM, and the target at 5 nM. The experiment was performed on LightCycler. LightCycler Ò LC24 Real-Time PCR System. All assays unless otherwise indicated were performed at least in triplicate.