In tail suspension, trunk curl and limb grasping were recorded with the mouse hanging and body tone, corneal/pinna/toe pinch reflex were observed while the animal was placed on a cage grid. Efficiency of grip was assessed by gently pulling the animal back by the tail. A wire manoeuvre was performed by letting the mouse hold on to a horizontal wire (length 45 cm, Ø 3.1 mm) with the forelimbs while being held by the tail and releasing it from a horizontal position.