For mitochondrial size determination, electron micrographs taken at 7.2K magnification were used, and mitochondrial sizes from 3-4 cells from each cell line were measured. Image J was used to determine the area occupied as pixel number, which was then converted to mm 2 . For determination of the number of mitochondria and autophagocytic vacuoles/vesicles, electron micrographs taken at low magnifications (1.9-3.6K), with the requirement of being able to fit an entire cell into the view, were used and 10-26 cells from each cell line were analyzed.