Nearly complete genomes and phylogenetic analysis of new bocavirus species. In a manner similar to HBoV1 and 2, all the new genomes of HBoV2, 3, and 4 encoded 3 large open reading frames (ORF). The left ORF encodes the nonstructural protein (NS), the middle ORF encodes NP1, and the right ORF encodes overlapping VP1/VP2 capsid proteins. Conserved motifs associated with rolling circle replication, helicase, and ATPase were identified within the NS protein. NP1 is a highly phosphorylated protein of currently undetermined function [38] ; NP1 differed in length between species, ranging from 214 to 219 amino acids. Situated within the VP1-unique (VP1u) region, the phospholipase A2 motifs required for parvovirus infectivity were found in all 6 genomes, together with the calcium-binding loop and catalytic residues.