Next, we characterized the dengue specific memory B cell response in donor 013, who had recovered from a primary DENV2 infection. We have previously reported on some of the properties of B cells and hMAbs generated from this donor [30] . Here we expand on these previous results by comparing properties of serum antibodies and hMAbs from this donor and by epitope mapping a subset of hMAbs from this donor. When immortalized B cell culture supernatants from donor 013 were screened for antibodies that bound to C6/36 insect cells infected with DENV2, 28% (567/2016) of the cultures were found positive for DENVspecific B cells following this initial screen ( Table 2) . From the DENV positive cultures only 2.9% of the cultures bound to rE protein (Table 2) . Thus, as in the case of donor 033, although dengue specific memory B cells were frequent, only a small fraction of the positive cultures from donor 013 produced antibodies that bound to rE protein.