Two random non-coding control siRNA were used: Sc-1 (59-rUrArCrGrUrArCrUrArUrCrGrCrGrCrGrGAT-3) from Qiagen, and Sc-2 (universal scrambled negative control siRNA duplex SR30004) from OriGene Technologies, INC. 48 h after transfection, cells were infected with Dengue virus at an MOI of 1. Briefly, cells were centrifuged (4006g, 10 min), the media was removed and 25 ml of RPMI containing the appropriate amount of virus was added and the plates were incubated for 45 min at 37uC. Then, 75 ml of RPMI with 10% FBS were added and cells were incubated at 37uC for the indicated hours. Subsequently, cells were recovered by centrifugation for 10 min at 4006g, and the cell pellets were lysed for RNA isolation.