Poster Exhibition -Miscellaneous Poster Session, Hall 5B Background: It is important to know the prevalence of ATP7B gene mutations of different geographical areas to justify the local screening strategies for Wilson disease (WD). Materials: Eleven unrelated Lithuanian families, including 13 WD patients were tested. Genomic DNA was extracted from whole venous blood using a salt precipitation method. Firstly, semi-nested PCR technique was used to detect the c.3207C>A (p.H1069Q) mutation. Patients not homozygous for c.3207C>A (p.H1069Q) mutation were further analyzed. The 21 exons of the WD gene were amplified in a thermal cycler. Direct sequencing of the amplified PCR products was performed by cycle sequencing using fluorescent dye terminators in an automatic sequencer. Results: Total of 13 WD patients (mean age 26.4 years; range 17-40; male/female, 3/10) presented with hepatic disorders and 16 their first degree relatives were studied. Some of WD patients in addition to hepatic symptoms have had extrahepatic disorders (haemolytic anaemia 3; Fanconi syndrome 1; neurophsychiatric and behavioural disorder 2). Twelve of 13 (92.3%) WD patients have had c.3207C>A (p.H1069Q) mutation, 6 of them in both chromosomes, 5 were presented as compound heterozygotes with additional c.3472 -82delGGTTTAACCAT, c.3402delC or c.3122G>A (p.R1041Q) mutation. For one patient with liver cirrhosis and psychiatric disorder no mutations were found. Out of 16 first degree WD relatives 11 (68.7%) were heterozygous for c.3207C>A (p.H1069Q) mutation. Conclusion: c.3207C>A (p.H1069Q) missense mutation is characteristic for Lithuanian WD patients. Even 92.3% of WD patients with hepatic presentation of the disease are homozygous or compound heterozygote for this mutation. Background: Diabetic dyslipidemia is a crucial problem of diabetic patients with inadequate control. We investigated the relationship between glutamic-pyruvic transaminase (GPT) and high-density lipoprotein cholesterol (HDL-C) in diabetic patients. Methods: With informed consents, we recruited outpatients with diabetes at a hospital in rural area in Taiwan in [2004] [2005] [2006] [2007] . Anthropometric measures, blood tests and urine screening were examined in diabetic patients. Results: Overall, there were 1241 diabetic patients aged 19-91 years enrolled in this study and 660 (53.2%) of them had low HDL-C. Diabetic patients with the highest quintile of GPT had higher average of body mass index (p<0.0001), diastolic blood pressure (p = 0.003), but lower average of HDL-C (p<0.0001) compared with diabetic patients with the lowest quintile of GPT. The prevalence of obesity (45.7% vs. 24.8%, p<0.0001) and low HDL-C (64.6% vs. 48.3%, p<0.0001) were higher in diabetic patients with highest quintile of GPT than in diabetic patients with lowest quintile of GPT. In the multivariate logistic regression, diabetic patients with highest quintile of GPT had higher odds ratio (OR) of low HDL-C compared with diabetic patients with lowest quintile of GPT (OR = 1.88, 95% confidence interval [CI] = 1.21-2.92). The corresponding OR of low HDL-C in patients aged 70 years and older was 3.30 (95% CI = 1.15-9.47). Conclusion: High GPT is one of factors associated with low HDL-C in diabetic patients.