Classically, palmitoylation dynamics have been studied using radiolabeled palmitate and pulse-chase protocols; for example, proteins or could instead reflect a different rate of palmitate turnover and subsequent Golgi recycling (Fig. 2) . Interestingly, proteins in this category, including Fyn, TC10, R-Ras, RhoB, and Rap2C all displayed colocalization with a Golgi marker when vesicular traffic through the secretory pathway was blocked by low temperature . This observation implies that after their synthesis, these proteins traffic via the Golgi and is consistent with the notion that the Golgi may be a specialized reaction center for the palmitoylation of all newly synthesized peripheral proteins and for a subset that undergo rapid depalmitoylation dynamics.