Method: We reviewed 130 HE stained slides for polarization. Sixty five amyloidosis cases were diagnosed by renal biopsy from 2008 to 2012 at our laboratory. All biopsies were evaluated by light and immunofluorescence microscopy. Slides were reevaluated blindly using a microscope (Olympus BX51) attached polarization filter and connected to a digital camera (Olympus DP21, SAL). Depositions which show green birefringence on HE with digitalized microscopy were considered as positive and results were confirmed using CR.