Furthermore, high levels of bacteria expressed rECP had 50 percent cytostatic effect on HL-60 and HeLa cells [31] , compared to non-affected controls. ECP was found binding the surface of HeLa cells and caused cell death after 24 hr, accompanied by increases in intracellular radical oxygen species (ROS) generation and caspase 3-like activity [125] . A mix of ECP and EDN purified from urine and incubated with the Kaposi's sarcoma cell line KS Y-1 for 16 hr caused complete cell death at 0.625 μg/mL while 1 μg recombinant ECP produced in yeast and incubated with the same time span decreased the viability of the KS cell line by 29 percent. Proteins expressed in yeast lack glycosylation and the possible implications of this were speculated [126] .