Results from the HTS, in which all 3 swabs were collected by trained HCPs, showed that the viral load on the second measurement could accurately and reliably be predicted from a loglinear model based on the first and third measurements. Applying the same approach to the IRIS data, we found that viral loads from self-swabs on the second measurement were very similar to the viral loads that we would have expected if the second swab had been collected by a trained HCP. Our results therefore support the feasibility and validity of using self-swabs as an alternative approach to permit laboratory confirmation of influenza-associated illnesses in a community setting.