Purified NAGK-20 and NAGK-37 were assayed for N-acetyl-Lglutamate kinase activity using Haas and Leisinger's method [56] , with modifications. The reaction mixtures contained 400 mM NH 2 OH?HCl, 400 mM Tris?HCl, 40 mM N-acetyl-L-glutamate, 20 mM MgCl 2 , 10 mM ATP and 2 mg of enzyme in a final volume of 1.0 ml at pH 7.0. After incubation at 25uC, 30uC, 37uC, 45uC, 50uC, 55uC or 60uC for 30 min, the reaction was terminated by adding 1.0 ml of a stop solution containing 5% (w/ v) FeCl 3 ?6H 2 O, 8% (w/v) trichloroacetic acid and 0.3 M HCl. The absorbance of the hydroxamate?Fe 3+ complex was measured with a spectrophotometer at A 540 [57] . Inhibition of the kinase activities of NAGK-20 and NAGK-37 were examined with and without 0.25, 0.5, 0.75, 1, 2.5, 5, 10, and 20 mM of L-arginine and incubated at 37uC for 30 min. One unit of N-acetyl-Lglutamate kinase is defined as the amount of enzyme required to catalyze the formation of 1 mmol of product per min under the assay conditions used. Each assay was performed in duplicate. Results were presented as means and standard deviations of three independent experiments. Figure S1 Physical map of the chemotaxis-related genes in L. hongkongensis. While the three gene clusters contain the transducer proteins and some of the methyl-accepting proteins (MCPs), most MCPs are scattered outside the clusters. Genes in orange are coding for chemotaxis transducer proteins; genes in green are coding for MCPs; genes in grey are coding for hypothetical proteins. The numbers refer to the coding sequences in the L. hongkongensis genome.  Malignant mesothelioma Malignant mesothelioma is a fatal asbestos-associated malignancy originating from the lining cells (mesothelium) of the pleural and peritoneal cavities, as well as the pericardium and the tunica vaginalis. The exact prevalence is unknown but it is estimated that mesotheliomas represent less than 1% of all cancers. Its incidence is increasing, with an expected peak in the next 10–20 years. Pleural malignant mesothelioma is the most common form of mesothelioma. Typical presenting features are those of chest pain and dyspnoea. Breathlessness due to a pleural effusion without chest pain is reported in about 30% of patients. A chest wall mass, weight loss, sweating, abdominal pain and ascites (due to peritoneal involvement) are less common presentations. Mesothelioma is directly attributable to occupational asbestos exposure with a history of exposure in over 90% of cases. There is also evidence that mesothelioma may result from both para-occupational exposure and non-occupational "environmental" exposure. Idiopathic or spontaneous mesothelioma can also occur in the absence of any exposure to asbestos, with a spontaneous rate in humans of around one per million. A combination of accurate exposure history, along with examination radiology and pathology are essential to make the diagnosis. Distinguishing malignant from benign pleural disease can be challenging. The most helpful CT findings suggesting malignant pleural disease are 1) a circumferential pleural rind, 2) nodular pleural thickening, 3) pleural thickening of > 1 cm and 4) mediastinal pleural involvement. Involvement of a multidisciplinary team is recommended to ensure prompt and appropriate management, using a framework of radiotherapy, chemotherapy, surgery and symptom palliation with end of life care. Compensation issues must also be considered. Life expectancy in malignant mesothelioma is poor, with a median survival of about one year following diagnosis. Malignant mesothelioma is a cancer originating from the lining cells (mesothelium) of the pleural and peritoneal cavities, as well as the pericardium and the tunica vaginalis [1] . Its distribution may be uni-or multifocal or may involve the lining cells in a continuous manner.