These data indicate that s-RT-MELT is compatible with high-throughput nano-technology detection formats and reiterates the advantage of de-coupling enzymatic selection from the detection step. Comparison of the throughput using conventional pre-screening method (dHPLC or dHPLC/Surveyor TM ) to s-RT-MELT (Table 1) indicates that s-RT-MELT is 1-2 orders of magnitude faster when a large number of samples (4100) are screened for mutations. If the multiplex s-RT-MELT format is adopted, the throughput can increase further.