Human d7EB cells were plated in 24-well plates at concentration of 2 ϫ 10 6 cells in 1 ml of growth medium. Cells were cultured overnight in the presence or absence of LPS, 1 g/ml. The supernatant was collected, spun down for 10 minutes at 500g to remove possible cell contamination, and injected i.v. into septic mice 1 hour after CLP at a volume of 200 L per mouse.