Protein sequences derived from GRA10, GRA15, SAG2C, SAG2D, SAG2X, SAG3, SRS9, BSR4, SPA, and MIC were analyzed for CD8 + T cell epitopes based on predicted binding affinity to HLA-A03 supertype molecules using ARB algorithms from immunoepitope database (IEDB) http://www.immuneepitope.org [40, 41] . A total of 34 unique peptides IC 50 < 50 nM of all ranked nonameric peptides were selected. All protein sequences were from ToxoDB 5.1. Quantitative assays to measure binding of peptides to HLA class I molecules are based on inhibition of binding of radiolabeled standard peptide. Assays were as described [42] . Concentration of peptide yielding 50% inhibition of binding of radiolabeled probe peptide (IC 50 [43, 44] .