Once we had these experimental data, we tried to develop a unifying view of them. The ÁG predicted by the Visual Omp TM 7.0 software was compared with the percentage of the total MFI for each probe and target combination, including dInosine-containing probes (Figure 2A , 3A Figure 4B shows the total number of nt in each target that originated from either Noro (white triangles) or InflA (grey squares) and that are outside the position of the 18 dInosines when hybridized with the Ino18 probe, compared with the MFI (from Supplementary Figure S1A) for each combination. and 4A). For the sake of simplicity, data from 5-nitroindole and N-wobble-containing probes were omitted. The results demonstrated that some probe/ target combinations that hybridized well in practice, had very low predicted ÁG values ( Figure 6B ), e.g. the InflA probe hybridizing to the 74%12F (43% of the total MFI, ÁG = -20.62), 74%15F (77% of the total MFI, ÁG = À26.7), 74%18 nt (55% of the total MFI, ÁG = -29.24), and 74%22 nt (61% of the total MFI, ÁG = -34.55) targets.