Monitoring of key physiological parameters is standard during mechanical ventilation of humans and should be aimed also in experimental research. These key parameters need to reflect both the pulmonary (e.g. tidal volume, airway pressure) and the cardiovascular (e.g. heart rate, blood pressure) consequences of MV as well as oxygenation and acid-base status. Although MV may affect all these parameters, these entities have rarely been assessed together in the same study in mice (Table 1) . Table 1 summarizes ventilation studies, in which either lung impedance was measured or ventilation was performed for at least four hours. The table reveals that many studies that have focused on lung mechanics examined only a relatively short period of ventilation [2] [3] [4] [5] and only one study fulfilled both inclusion criteria [6] . Interestingly, studies in which mice were ventilated for more than three hours often provided cardiovascular parameters, but neglected the examination of lung functions [7] [8] [9] [10] . Some studies even completely lacked physiological parameters, although in several cases the authors referred to preliminary experiments that were not included in the published data [11] [12] [13] [14] . We believe that, without comprehensive information on physiological parameters, it is difficult to properly assess, standardize and compare the various ventilation strategies.