We compared the mean substitution rates derived from BEAST analyses for ten individual genes obtained from ALN2. Settings were as described above with an additional uniform prior distribution on the time interval [360-10000] fitted to the root height. This prior captures the background knowledge that crown radiation of flaviviruses occurred after the end of the last glaciations (placed 10,000 years ago) and that the Tick-borne encephalitis virus emerged before the divergence of two of its inclusive clades namely LIV and W-TBEV whose split was estimated to be earlier than 360 years ago [38] , placing the species divergence within this rather wide interval.