Since viral RNA is able to induce IFNb gene transcription ( Figure 5B and 5F) we again wanted to rule out that induction of SOCS-3 by viral 59 triphosphate RNA is mediated by auto-or paracrine action of de novo synthesized IFNb. In order to do so, cells were stimulated with viral RNA after treatment with the protein synthesis inhibitor anisomycin at two different concentrations ( Figure 5G ). SOCS-3 mRNA was still induced to the same extent in the presence of the protein synthesis inhibitor, providing the ultimate proof that de novo protein synthesis is not required for SOCS-3 induction.