In a second study by Kakuda's research group, food containing 5% banaba water extract was used to feed female obese KK-Ay/Ta Jcl mice. Obese mice treated with banaba extract had a significantly reduced body weight (10%) compared with control mice fed with a regular diet (20) . No change in food intake was observed (20) . Interestingly, it was also discovered that liver triglyceride content was reduced by more than 40% in the banaba extract-treated mice. In addition, the parametrial adipose tissue was 10% lighter (P50.01) (20) . However, as in the previous antidiabetic activity study of BE in animals (19) , both the identity of the effective component(s) and the mechanism for the activity were not studied. Nevertheless, these two studies clearly demonstrated the in vivo antidiabetic and anti-weight gaining efficacy of the extract.