In plants, luteovirids move from cell-to-cell and long distance as virions. However, in plants luteovirids must also replicate, assemble into virions, thwart the plant immune response and exhibit phloem-tissue tropism for host-to-host transmission by aphids, processes which likely involve the recruitment of a wide range of host factors to accomplish [21] . Interestingly, Bencharki and colleagues showed that the addition of soluble proteins to the aphid diet, including phloem-specific proteins that can interact with virions, can enhance transmission efficiency of the polerovirus, cucurbit aphid borne yellows virus [22] . Thus, we hypothesize that plant host proteins are also involved in aphid transmission. Serendipitously, we discovered that common additives to the plant tissue homogenization buffer during virus purification do not appear to alter the virus morphology or protein structure, but do render the virus non-transmissible by aphids. By comparing the host plant proteomes associated with transmissible and non-transmissible virions, proteins with potential involvement in transmission were identified. Moreover, our data indicate an increased accumulation of several virus-associated host proteins within aphids that have fed on infected plants, supporting a role for these host proteins in the circulative transmission process.