Hybridization conditions can also be used to increase specificity and sensitivity. Temperature and formamide concentration can be modified to adjust stringency thus altering specificity . A lower hybridization solution volume and mixing during hybridization (Adey et al., 2002) have both been shown to increase sensitivity. Decreasing ozone levels, which can degrade cy-dye signal (Branham et al., 2007) , can also improve sensitivity.