The ability of the Endo IV assay to detect different target concentrations is shown in Figure 6 . It was observed that in the case of 125 nM target concentration, the probe-based fluorescence plateaued in $1 h, while in the presence of 25 nM target a fluorescence plateau was reached in $3 h. The limit of detection of this assay is 0.04 nM, clearly distinguishable from the stable background ( Figure 6 ). This stable background, a characteristic feature of the assay, is maintained over a period of 15 h. This property is also observed in the assay with other nucleic acid targets.