In this paper, we present a method to extract the values of viral kinetics parameters, specific to a particular strain, from parallel experiments of plaque and viral yield assays. Our previous study [22] assessed the in vitro replicative capacity and fitness of pairs of WT influenza virus strains and their H275Y mutant counterparts by use of viral yield assays and by qualitatively comparing plaque sizes. Here, we show that it is possible to use these experimental measures to quantitatively characterize the kinetics parameters responsible for the replicative efficiency of influenza virus strains. As a proof of concept, we apply our method for extracting the viral kinetics parameters to the oseltamivir-susceptible/-resistant pair of A/Brisbane/59/2007 influenza virus strains in order to determine how the known genotypic differences in these two strains map to quantitative changes in the viral kinetics parameters characterizing their replicative efficiency. The kinetics parameters extracted through our method suggest that the H275Y mutant has weaker NA activity compared to its WT counterpart -confirmed by NA activity assays -which manifests itself as a longer phase of latent infection before viral release -confirmed by single-cycle viral yield experiments. However, the results also indicate that this longer latent infection period for cells infected by the H275Y mutant is compensated for by a shorter infecting time required for that cell, once releasing virions, to successfully infect other cells.