Conclusions: RFA was effective for HCC < 3cm in diameter and DCP < 40 mAU/ml. Hepatic resection should be selected for single HCC with DCP > 40 mAU/ml even though HCC <3cm in diameter. Background: Radiofrequency ablation (RFA) is now a common treatment for small hepatocellular carcinoma (HCC). However, critical complications after RFA such as rapid intrahepatic dissemination have been reported. In this study, we investigated the method how to estimate the malignant potential of small HCC by dynamic CT before RFA. Methods and results: Firstly, 61 HCCs less than 3cm in diameter were analyzed. Those tissues were classified into 4 groups as followed, small nodular type with indistinct margin (type E), simple nodular type (type 1), simple nodular type with extranodular growth (type 2), confluent multinodular type (type 3). In the type 2 and 3 groups, portal invasion over vp1 were observed more frequently than those in the type 1 and E groups. At the next step, these 61 HCCs were classified into above-mentioned 4 types by two radiologists according to the shape of early stain or defect of delay phase of dynamic CT before operation. The accorded rate was 87% between those classifications. Next, 45 patients, which had solitary HCC less than 3cm in diameter and treated with RFA, were classified into those 4 types by dynamic CT before RFA. The recurrence rate and prognosis of those patients were examined. In the type 2 and 3 groups, the recurrence rate was higher and significant worse prognosis was showed than those in type 1 and E group . Conclusion: It was suggested that HCC with type 2 and 3 might process higher malignant potential and RFA should be carefully performed on those types of HCC. Background/Aims: Hypoxia-inducible factor-1 (HIF-1 ) is the central transcriptional factor in the cellular response related to various aspects of cancer biology, including proliferation, survival, angiogenesis, and extracellular matrix metabolism to hypoxia. IL-8 became known to replace HIF-1 functions in the other cancer cell lines. The aim of this study was to evaluate whether IL-8 may induce angiogenic factors without HIF-1 by inflammation signal of hypoxic condition. Methods: HIF-1 knockdown cell lines of HCC (Huh7 and HepG2) were constructed by RNA interference tools, and cultured under normoxia (21%O2, 24 hours) and hypoxia (1%O2, 24 hours) conditions. Following transfection, the amounts of HIF-1 , IL-8, angiogenic factors and matrix metalloproteinase (MMP) were examined using RT-PCR and western blotting, respectively. Results: The expression of HIF-1 , angiogenic factors, MMP, IL-8 was markedly enhanced in wild types that were cultured under hypoxia, and the hypoxic induction of angiogenic factors and MMP was partially blocked in HIF-1 knockdown HCC cell lines. NF-B inhibitor suppressed angiogenic effects by blocking IL-8 activity. Conclusion: These data suggest that IL-8 induced tumor angiogenic factors in HIF-1 knockdown HCC cell lines. Background There were some reports that liver caner related to the levele of sera HBVDNA Our research focused on the relationship between the quantity of hepatocellular HBV cccDNA , tDNA and liver cancer. Methods The samples included the liver tissue of 24 CHB patients (CHB group) and the para-liver cancer tissue of 26 primary liver cancer patients (PHC group) The quantity of hepatocellular HBVcccDNA, tDNA were assayed by FQ-PCR in both groups. Result: The quantity of hepatocellular HBV cccDNA in CHB group was 11.56±16.14 copys/cell higher than PHC group(3.96±9.27 copys/cell), P=0.011; The quantity of hepatocellular HBV tDNA in CHB group was 57.38±91.64 copys/cell higher than that of PHC group(35.07±93.03 copys/cell),P=0.13.; Conclusion: The quantity of hepatocellular HBVcccDNA, tDNA can not be used as predictors of liver cancer for hepatitis B patients. Hepatic cancer predominantly occurs in Males. This is almost a commonsense to most of us. But the detailed mechanisms underlying such phenomenon are still not well-known. The average age of liver cancer patients are about 30-40 years old. So most female patients have udergone pregnancy at least one time. Pregnancy is a very important event before or during the development of liver cancer in females. In this special period, not only sex hormones secrete in a strange manner, but also immune system functions in a special module which is very different from normal. So it is urgent to investigate the impact of process of pregancy on the development of hepatic cancer. In this study, 100 female SD rats are randomly divided into two groups: pregance group and controll group. Rats in both groups are injected IV Diethylnitrosomine(a chemical carcinogen). In pregnance group, rats are raised together with male rats in 3:1 ratio(3 female, 1 male) to make every rats undergo pregnancy. While in controll group, rats are coupled with spermaduct-ligated male rats. The size and amount of hepatic cancers in pregancy group are smaller and less than those in controll group. The survial rate is also significantly higer than that in controll group. We conclude that the process of pregnancy exerts an inhibitory role in the development of chemical induced hepatic cancer in rats. Acknowledgement: This project was sponsed by the National Natural Science Foundation of China. The number of the grant is 30600727: