The consensus full-genome sequence of TEV-At17 was obtained following standard methods. In short, RNA was extracted using the RNeasyH Plant Mini kit (Quiagen), it was reverse-transcribed using MMuLV polymerase (Fermentas) and PCR amplified with Taq polymerase (Roche). The ABI Prism Big Dye Terminator Cycle Sequencing Kit 3.1 (Applied Biosystems) was used for cycle sequencing with fluorescently labeled dideoxynucleotides. Cycle sequencing reactions were carried out on a GeneAmp PCR System 9700 thermal cycler (Applied Biosystems). Labeled products were resolved in an ABI 3100 Genetic Analyzer (Applied Biosystems). Seven pairs of specific primers were used to amplify the 9.5 kb of TEV genome. The resulting fragments were overlapping, facilitating the task of fragment sequence assembly. Sequences were processed and analyzed with the STADEN 1.4b1. The 59-and 39-ends were sequenced by the RACE-PCR method [39] .