We further evaluated the potential of treatments with an FMDV-specific rS. cho and rAd5 mixture in guinea pigs. Three of the five animals in the group treated with the p3D-NT56/S. cho+Ad5-POL mixture were completely protected as determined by the absence of vesicles on their feet (Tab. I). No significant increase in antiviral effect was achieved in this group when compared to the group treated with p3D-NT56/S. cho alone (Tab. I). This indicated that a simple combination of two vectors could not improve the protection of animals.