Five vertebrate genes and all invertebrate were excluded from the phylogenetic analysis and instead classified into subfamilies by using a BLAST approach (Table S1) . Some genes could not unambiguously be classified into the vertebrate subfamilies: C. intestinalis (1 gene), S. mansoni (1 gene), B. floridae (2 genes), S. purpuratus (3 genes), N. vectensis (1 gene) and M. brevicollis (1 gene). By combining the results of the phylogenetic analysis and BLAST classification, we created a schematic overview of the organisms' gene repertoire and a schematic picture of the Dispanin family's evolutionary history, which suggests that the invertebrate Dispanins share more similarity towards the DSPC and D subfamilies than DSPA and B ( Figure 2 ).