Using the experimental conditions described in the 'Methods' section, we tested the Poxviridae 16-plex (Table 4 ) against vaccinia virus, Lister strain DNA. As assessed by agarose gel analysis, we achieved specific amplification of the predicted 617-bp amplicon for the target vaccinia genome (lane 3, Figure 7 ). The band does not appear in the no template control shown in lane 4 ( Figure 7 ).