The aim of this review was to briefly describe the current state of the art of diagnostic devices for isothermal nucleic acid amplification. The isothermal strategy has been a versatile and powerful technique applied in the detection of microbial and viral pathogens, among many other uses in the diagnostic laboratory. The combination of the properties derived from isothermal amplification and biosensing platforms proved a valuable strategy for simplifying the analytical science of nucleic acid detection. In reviewing the various detection configurations, we observed that integrated microchip systems are particularly desirable because these systems provide significant advantages in convenience and cost-effectiveness, simultaneously simplifying operational procedures and shortening analysis times.