The pwLAMP was found to have comparable sensitivity as the conventional LAMP at 60uC as both assays were able to detect 300 copies of IS2404 element (equivalent of 1.5 genomes of M. ulcerans). The detection limit of the conventional LAMP at 65uC improved to 30 copies of IS2404 and this level of sensitivity may probably be achieved with a pocket warmer that can attain a temperature of 65uC and maintain a holding time of 60 min. When applied to purified DNA extracts of clinical specimens, the pwLAMP, conventional LAMP and IS2404 PCR yielded concordant results (Tables 1 and 2) .