Wild-type and Bid-deficient cells in 24-well plates were transfected with 0.72 mg/well of an NF-kB reporter plasmid, which expresses firefly luciferase under NF-kB control (pNF-kB-Luc), and 0.08 mg/well of control plasmid pRenilla-Luc, which expresses Renilla luciferase constitutively, using Fugene6 (Roche). After incubation for 24 h, transfected cells were adsorbed with reovirus in serum-free medium at room temperature for 1 h and incubated at 37uC in serum-containing medium for 24 h. Luciferase activity in the cultures was quantified using the Dual-Luciferase Assay Kit (Promega) according to the manufacturer's instructions.