As an independent approach to mapping EDIII-reactive hMAbs, we used a yeast surface display assay that has been previously used to map numerous flavivirus antibodies [22, 23, 33] . Table 3 summarizes all mutations that resulted in loss of binding or neutralization of EDIII antibodies generated from donor 013. All the human EDIII-binding MAbs mapped to the lateral ridge Table 3 ) (orange). Ribbon diagram of DENV-2 EDIII was generated from a published X-ray crystallographic structure. The disulfide bond is highlighted in yellow. Human MAbs 3.7 and 25.5 are type-specific antibodies that bind to epitopes centered on the lateral ridge while 10.16 is sub-complex-specific and bind to an epitope centered on the A strand of EDIII. doi:10.1371/journal.pntd.0001188.g004