Total white blood cell count (WBC), and peripheral polymorphonuclear neutrophil count (PMN) were assessed using the Cell-Dyn Sapphire haematology analyzer (Abbott, Chicago, Il, USA). Serum creatinine was measured with a modified jaffe method on Architect ci16200 analyzer (Abbott, Chicago, Il, USA). High-sensitivity CRP (hs-CRP) was measured with a turbidimetric assay on the same analyzer. Troponin-I was measured with a chemiluminescent immunoassay on the same analyzer. Serum-NGAL was measured with an ELISA (Bioporto, Gentofte, Denmark).