Subjects and Methods: Subjects were 3633 males who underwent medical checkups in both 2005 and 2007 (mean age 56.9± 11.2). Among these subjects, 959 were non-drinkers (ND), 1574 were light drinkers (LD: less than 140 g of alcohol /week), 943 (26.0%)were moderate drinkers (MD: 140-420 g/week), and 157(4.3%) were heavy drinkers (HD:over 420 g/week). We examined the relationship between the amount of alcohol intake and laboratory data by analysis of variance. We provided these subjects motivational support using medical data on the checkup day, and sent further advice 2 weeks later. We analyzed changes in the amount of alcohol intake and laboratory data 2 years later. Result: γ-GTP (IU/L) values in 2005 were ND (34.9±1.2), LD (44.5±1.2), MD (66.7±5.4), and HD (96.5±5.8). There were significant differences among the groups, except for that between ND and LD. The amount of alcohol intake was decreased in both MD&HD 2 years later. The γ-GTP, GPT, and TG values of 1100 subjects of MD or HD decreased significantly. Conclusions: One third of the subjects had inappropriate drinking behaviors, although they were sufficiently health-conscious to come for a medical checkup. After receiving motivational support, the amount of alcohol intake decreased and the laboratory data improved.