Combining various theoretical methods like pharmacophore modeling, database screening, molecular docking and DFT calculations, an investigation for identification of novel chymase inhibitors and to specify the key factors crucial for the binding and interaction between chymase and inhibitors was performed. The highly correlating (r = 0.942) pharmacophore model (Hypo1) with two hydrogen bond acceptors, and three hydrophobic aromatic features was generated. After successfully validating "Hypo1" using test set and Fischer randomization methods, it was further used in database screening. Hit compounds were subjected to various drug-like filtrations and molecular docking studies. Finally, three structurally diverse compounds with high estimated activity and strong molecular interactions with key active site amino acids were identified. Furthermore, a DFT study, which articulated the influence of the electrostatic features of compounds on their inhibitory activity well, was performed. Analysis of orbital energies and plots of MESP has shown very clear trends between electronic properties and inhibitory activity (IC 50 ) data. An increasing trend was observed between IC 50 and HOMO energy values. The molecular electrostatic potential features were also consistent with the key chemical features of "Hypo1" thus successfully validating "Hypo1" by the DFT method. Therefore, the results of this study will be helpful, not only in the development of new potent hits for chymase, but also in providing a better understanding of the interaction between the chymase and inhibitors. This will in turn assist in the rational design of novel potent enzyme inhibitors. Perspectives on Immunoglobulins in Colostrum and Milk Immunoglobulins form an important component of the immunological activity found in milk and colostrum. They are central to the immunological link that occurs when the mother transfers passive immunity to the offspring. The mechanism of transfer varies among mammalian species. Cattle provide a readily available immune rich colostrum and milk in large quantities, making those secretions important potential sources of immune products that may benefit humans. Immune milk is a term used to describe a range of products of the bovine mammary gland that have been tested against several human diseases. The use of colostrum or milk as a source of immunoglobulins, whether intended for the neonate of the species producing the secretion or for a different species, can be viewed in the context of the types of immunoglobulins in the secretion, the mechanisms by which the immunoglobulins are secreted, and the mechanisms by which the neonate or adult consuming the milk then gains immunological benefit. The stability of immunoglobulins as they undergo processing in the milk, or undergo digestion in the intestine, is an additional consideration for evaluating the value of milk immunoglobulins. This review summarizes the fundamental knowledge of immunoglobulins found in colostrum, milk, and immune milk. The topic of immunoglobulins in milk immediately brings to mind the relationship between mother's milk, transfer of passive immunity from mother to neonate, and the immature immune system of the neonate. Research in this field dates back to the late nineteenth century, however for many centuries herdsmen have capitalized on the linkage between maternal immune status and the immunological protection and development of the neonate [1, 2] . Immunoglobulins in mammary secretions come from several sources and represent a history of the antigen exposure of the mother and the response of her immune system. Immunoglobulins are transported through the mammary epithelial cells by receptor-mediated mechanisms and transferred out of the mammary gland by milk ejection during suckling. The immunoglobulins then enter the environment of the gastrointestinal tract of the neonate. Although that environment is primarily geared toward digestion to gain nutritional benefit, the immunoglobulins remain sufficiently stable to provide protective benefits for the neonate, either through uptake into the vascular system in the newborn of some species or through immunological function in the gastrointestinal tract. The immunoglobulins found in milk and the transfer of passive immunity from mother to neonate have been reviewed by many authors, with a partial listing referenced here [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] .