3.6. Cellular Uptake of pDNA/AF-Liposomes. Next, we examined the cellular uptake of pDNA/γ-CyD/AF-liposomes into HepG2 cells using a CLSM. Figure 11 shows the CLSM images for distribution of RH-AF-liposomes and Alexa-pDNA in HepG2 cells at 3 h after transfection. The strong fluorescence derived from RH-AF-liposomes and Alexa-pDNA in the presence of γ-CyD was mainly observed in cytoplasm of HepG2 cells. Meanwhile, the fluorescence RH-AF-liposomes and Alexa-pDNA in the absence of CyD and with HP-γ-CyD was mainly observed on cell surface. Hence, these results suggest that pDNA/γ-CyD/AF-liposomes can be internalized into HepG2 cells to a larger extent, compared to pDNA/AF-liposomes and pDNA/γ-CyD/AF-liposomes.