As discussed before, the probe for ADA (Adenosine deaminase) is the first that has a different trend. Since we put all metastasis samples together in the same group when we calculated the average probability profile (and we have a heterogeneous group) we have on our ranking 58 probes that appear before ADA (we refer to the Supplementary File Haqq-PLoSONE-SupFile.xls). An analysis using GATHER (http://gather.genome.duke.edu/) [155] to interpret the collective influence of the lack of expression of all these genes in the metastasis samples reveals an interesting new perspective. Using Gene Ontology, we found that six of the 44 genes identified by GATHER are related to epidermis development (CDSN, DSP, EVPL, GJB5, KRT13, KRT5), p-value ,0.0001, Bayes Factor 16, and eight genes are related to cell adhesion (CDSN, CLDN1, DSG1, DST, LGALS7, LRIG3, PCDH21, PKP1), p-value,0.0001, Bayes Factor 7. ANK1 (Ankyrin 1, erythrocytic), AA464755 was also singled out as by our Gene Ontology analysis as related to the maintenance of epithelial cell polarity (p-value = 0.002, Bayes Factor 3). The use of another profiler of genome signatures (g:Profiler, [156] ) also reinforces the view that many genes that have lost expression are related to 'Epidermis Development' (COL17A1, DSP, EVPL, GJB5, KRT13, KRT5, LCE1C, MAFG, TGM3) with p-value = 7.78E-11. Thirteen are associated with Gene Ontology function of cell communication (ANK1, CDSN, CLDN1, DSG1, DST, GCHFR, GJB5, GPR115, LGALS7, LRIG3, PCDH21, PKP1, PTGER3), albeit with a pvalue of only 0.02. GCHFR is also involved in nitric oxide metabolism.