To confirm that the amplicon observed in gel analysis was a specifically amplified product from vaccinia virus, Lister strain, we sequenced the product according to the procedures described above. An analysis of the high-quality sequence read data taken from the electropherogram yielded a 95% identity (maximum) to vaccinia virus, Lister strain (AY678276.1) with a query The forward and reverse primers (FP, RP) in bold are predicted to amplify vaccinia Lister with the indicated product length (617-bp amplicon size).