Methods: liver sample were paraform-glutaral fixed, paraffin-embedded, sectioned and immunohistochemical stained, and positive samples were selected for histological analysis and RT-PCR detection. Result: Positive rate of HEV Immunohistochemistry ranged from 90% to 100% (Fig. 1) . Hepatocyte degeneration, scattered singled karyopyknosis, lymphocytic infiltrate, hyperplasia of bile canaliculus at the portal area and fibrous connective tissue hyperplasia been observed during histological analysis (Fig. 2) , and two genotype 4 HEV which closely related to many strain isolated from patients with sporadic acute hepatitis been detected.