Preparation of CD11c + cells and CD4 + -and CD8 + T cells CD11c + spleen cells of naïve mice and CD4 + T and CD8 + T spleen cells of mice immunized with OVA-liposome conjugates were prepared with the magnetic cell sorter system MACS, according to the manufacturer's protocol using anti-CD11c, anti-CD4 and anti-CD8 antibody-coated microbeads (Miltenyi Biotec).