Lentiviral vector derived viruses were obtained by transfection of HEK 293T with 3 plasmids encoding STING, HIV-1 Gag-Pol, and VSV-G respectively [55] . Viral supernatants were harvested 48 and 72 hours post-transfection, 0.45 mm filtered, concentrated at 14,000 g for 6 hours over a 20% sucrose cushion and frozen at 280uC until used.