The state of glycosylation of the urinary ACE2 proteins at 120 kDa and 90 kDa was studied by treating urine samples with the deglycosylase enzyme PNGase F. Urinary ACE2 fragment sizes were reduced to ,85 kDa and ,65 kDa by PNGase F treatment, indicating that both fragments were originally glycosylated ( Figure 2D ).