All cells were washed twice and scraped into ice cold PBS, where crosslinking were preformed Huh7 cells were cross-linked with dimethyl 3,39 dithiopropionimidate (DTBP, 5 mM) for 30 min on ice prior to scraping. Cells were pelleted before resuspending in ice-cold RIPA lysis buffer (0.4% (v/v) with proteinase inhibitor cocktail). Lysates were passed through a 22G needle 5 times and re-cleared by centrifugation at 116006g for 2 min.