To characterize the IFITM gene repertoires in vertebrates, we searched 27 vertebrate genome sequences with high genome coverage ($66) or representing the major evolutionary lineages in vertebrate phylogeny (such as opossum, lizard, platypus, etc.), using previously described IFITM sequences as queries. The taxa included ten non-mammalian vertebrates (five fishes: stickleback, tetraodon, medaka, fugu, zebrafish; one amphibian: frog; one nonavian reptile: lizard; three birds: chicken, turkey, zebra finch) and 17 mammals covering six primates (human, chimpanzee, gorilla, orangutan, macaque, marmoset), four glires (mouse, guinea pig, rat, and rabbit), five other mammals (tree shrew, cow, horse, dog and elephant), 1 metatherian (opossum) and 1 prototherian (platypus). We divided the newly identified genes into three types based on the following criteria: (i) functional genes, which contain full-length ORFs with intact CD225 domain and C-terminal TMDs; (ii) putative functional genes, which have CD225 domains but contain incomplete ORFs; and (iii) pseudogenes, which are sequences with pre-mature stop codons.