Mice were bled and then sacrificed via anesthetic overdose and organs removed aseptically. Organs were homogenized in 500 mL (100 mL for lymph nodes) of buffer containing 137 mM NaCl, 20 mM Tris-Cl pH 8.0, 5 mM EDTA, 0.05% Triton-X 100, and protease inhibitor cocktail (Roche). Serum and organ homogenate supernatants were assayed for IL-1b by ELISA (R&D Systems). Organ IL-1b amounts were normalized to the amount of protein in the samples, as determined using a bicinchoninic acid (BCA) protein assay (Thermo Scientific).