Cuprizone ingestion results in a reproducible pattern of extensive corpus callosum demyelination (12, 13) . Cuprizone treatment was started at 8 weeks of age and only male mice were used. Cuprizone (0.2% (w/w), finely powdered oxalic bis(cyclohexylidenehydrazide) (Sigma-Aldrich, St. Louis, MO), was thoroughly mixed into chow (diet TD.01453; Harlan Teklad, Madison, WI), which was available ad libitum. Mice were maintained on the cuprizone diet until perfused for analysis or returned to normal chow after 6 weeks or 12 weeks of cuprizone ingestion.