The effects of temperature and translational inhibitors were assessed by standard 10-fold dilution spot assays. Yeast were grown in H-tryptophan synthetic deletion (SD) media (-Trp) to mid log phase. OD 595 values were obtained, and cells were serially diluted 10-fold from 10 5 to 1 CFU per 2.2 ml and spotted on -Trp plates. Growth was monitored at 20 C, 30 C and 37 C, and pharmacogenetic assays utilized 2 mg/ml paromomycin, 40 mg/ml anisomycin or 30 mg/ml sparsomycin incubated at 30 C for 3-5 days. Killer virus assays were performed as previously described (19) .