Histopathological and immunohistochemical analysis of wildtype mice infected with 10 4 PFU ZH548 RVFV was performed 3 to 5 days after i.p. inoculation. rZHDNSs-hRLuc-infected Ifnar1 2/2 mice were euthanized at 8, 16 and 34 h after i.p. inoculation. For each time point, a complete post-mortem examination was carried out. The lung, brain, kidneys, spleen, liver, pancreas, thymus, testis, uterus and ovaries were removed and immediately fixed for one week in 10% neutral buffered formalin. Samples from each organ were embedded in paraffin and five-micrometer sections were then cut and stained with hematoxylin and eosin (HE). The histological characterization of lesions was completed by an immunohistochemical detection of the RVFV using mouse antibodies against the RVFV (dilution 1:100) visualized with the Histofine Simple Stain MAX-PO kit (Histofine Biosciences inc, Cambridge, UK).