Currently the isolation of C. albicans from blood cultures is the standard method for the diagnosis of candidemia. Nevertheless, blood cultures may only become positive late in infection, and in one study up to 50% of all autopsy-proven cases of candidemia were reported as negative in blood cultures [14] . Thus, the ability to rapidly and easily diagnose candidiasis is urgently needed. An alternative approach to microbiological confirmation of C. albicans infection is serological diagnosis. An immunoproteomic approach using two-dimensional electrophoresis followed by quantitative Western blotting and mass spectrometry has been used to profile serologic response to peptides from cell surface extracts in candidemia [15] [16] [17] . A significant proportion of antigens identified were glycolytic enzymes and heat shock proteins. An antigenic multiplex consisting of the peptides Bgl2, Eno1, Pgk1, Met6, Gap1, and Fba1 provides 87% sensitivity and 74% specificity when distinguishing patients with candidemia from uninfected hospital patients [17] . However, this approach has several limitations; only the most abundant and soluble proteins can be resolved on the immunoblot, there is a lack of reproducibility of cell wall preparations, and most importantly, there is the inability to account for various stage-and tissuespecific gene expressions from the cultured cells. These limitations can be addressed by using a protein microarray to profile antibody responses [18] [19] [20] [21] .