Background: The treatment in chronic hepatitis C virus (HCV) is not highly effective, and cost, duration, and side effects are challenging. Predicting favorable factors of response to treatment would make it possible to give it only responsive patients. Recent studies report more conclusive results about the role of apoptosis in inflammation and fibrosis seen in chronic viral hepatitis. Hepatocyte damage in HCV is mediated by cytotoxic T-cells. Apoptosis primarily developed by the interaction between Fas antigen on hepatocyte and Fas ligand on T-cell corresponds to a main mechanism for hepatocyte damage. Methods: In this study, we aimed to detect any relationship between apoptotic markers (Fas, Fas ligand, Fas-associated death domain, caspases 3,8, and 9, insitu apoptosis) in liver biopsy taken before the treatment and response to the treatment of interferon+ribavirin. Additionally, any relationship between these parameters and the other ones predicting the response to therapy including ALT level, viral load, genotype, and gender were studied. Results: The study includes the patients in 4 centers managing chronic HCV infection. All parameters were studied in 180 patients. Study results revealed that histological activity index is correlated with CD95 staining density, caspase 8 intensiveness, and portal and parenchymal Fas ligand scores. Fibrosis is also seen to be correlated with the same parameters. Apoptotic parameters of the responsive cases were not significantly different from unresponsive ones. Conclusion: Apoptotic parameters studied in the liver tissue is associated with inflammation and fibrosis, however these parameters may not predict the response to the treatment.