Primer for renalase gene was designed as Sp 59-ATAA-GAATGCGGCCGCATGGCGCAGGT GCTGATC -39, As 59-GGAAGATCTCTAAATATAATTCTTTAAAGCTT -39, and then renalase gene was replicated using RT-PCR technique. Renalase gene was then inserted in pBudCE4.1 (Invitrogen, Carlsbad, CA, USA) vector plasmid, after which the immunization plasmid was completed.