The LFA is an appropriate technology on which to base a rapid assay. The technique permits rapid diagnosis, allowing time for the early implementation of control measures to reduce the possibility of spread of FMD. The LFA has been developed widely to support clinical diagnosis of different diseases [225] [226] [227] , including FMD [170, 228] . A rapid lateral-flow assay (LFA) based on FMDV antigen detection, which is easy to use and can be utilized on the farm to reduce the time required for transport and laboratory diagnosis. The detection of FMDV antigens by direct application of vesicular fluids and epithelial suspensions from animals of an infected farm may reduce the chances of diagnostic error arising from nonspecific reactions. Oem et al. showed that the diagnostic sensitivity of the LFA for FMDV types O, A, C, and Asia 1 was similar, at approximately 87.3%, to that of 87.7% obtained with antigen enzyme-linked immunosorbent assay (AgELISA). But the specificity of the LFA was 98.8%, compared to 100% for the AgELISA [122] . Recently a lateral flow device (LFD) for the detection of footand-mouth disease virus (FMDV) of the SAT 2 serotype was developed using a monoclonal antibody (Mab 2H6) for providing rapid and objective support to veterinarians in their clinical judgment of the disease and for specific confirmation of a FMDV type SAT 2 infection [229] .