Conclusion: Lodox X-ray is a proven invaluable special investigation in forensic, and also academic, autopsy examination procedures. Sunday, 9 September 2012, 17.00-19. Objective: Castration resistant prostate cancer (CR-PCa) is the most aggressive form of prostate cancer (PCa) posing a significant therapeutic challenge. Our aim was to perform whole exome-sequencing on 5 CR-PCa/normal paired formalin fixed paraffin embedded (FFPE) samples using the SOLiD4 next generation sequencing platform (NGS). We identified two promising genes-YWHAZ and PTK2, that could serve as novel potential therapeutic drug targets in PCa. Method: Genomic DNA was sequenced from 5 CR-PCa/ normal paired FFPE samples. A set of amplified/deleted genes were validated using fluorescence in-situ hybridization (FISH) assays using a PCa progression cohort consisting of 138 cases for localized PCa, 105 patients with primary PCa and corresponding LN metastasis, and 39 samples for CRPCa. Results: Exome-sequencing identified regions of deletions/ amplifications, including NKX3.1, PTEN, CMYC and AR genes, known to play a role in PCa. We identified several amplified genes as druggable targets such as PTK2 and YWHAZ. For YWHAZ we identified 3.5 % amplification in localized PCa, 24.2 % amplification in LN metastasis and 45.5 % amplification in CRPCa. For PTK2 we 4 % amplification for localized PCa and 34 % for both LN metastasis and CR-PCa.