Intersection realizes the concept of mutual verification from both methods by creating a new set that consists of only the common epitopic amino acids in both A and B. Hence, Intersection should be more reliable than either method in epitope detection if its outcome is positive. Without confirmation from real experiment results, mutual verification from artificial prediction methods can only provide an indication of where the true epitopic region is likely located on the antigen surface. Ideally if both methods produce exactly the same epitopic amino acids, both Intersection and Union should return the same set of epitopic amino acids. Hence MimoPro and Pep-3D-Search share a consistency of 100% to each other on the case However, this failure in mutual verification only means that the two methods cannot support each other on the case under study, but it does not mean that the predicted epitopic regions by either method are not related to the genuine epitope. Other approaches It can be clearly seen on these images that Intersection provides more confined prediction as most of the residues lie on the interface whereas Union outlines a larger area that may cover (part of) the potential epitopic region. doi:10.1371/journal.pone.0037869.g004 are needed to verify the epitopic regions predicted by either method.