Amplicons 601 bp in length were obtained in the RT-PCR assay aimed at detecting all TAstV types. Amplicons were purified using a NucleoSpin Extract II Kit (Macherey Nagel, Germany) according to the manufacturer's instructions and sequenced at the commercial service Genomed (Poland) . Sequencing was performed in both directions with the same primers that were used for RT-PCR reactions. Using the Seqman program (DNASTAR, Madison, WI), the forward and reverse nt sequences were edited, cured and aligned as one consensus sequence. Multiple alignments of nt and aa sequences were performed using the MegAlign application (DNASTAR, Madison, WI) using the Clustal W method. Phylogenetic analysis of aligned sequences was performed with MEGA 4.1 using the neighbor-joining method with the maximum-likelihood model. Bootstrap scores were generated from 1000 replicates. The nt sequences were translated to deduced amino acid (aa) sequences, which were also compared to detect any changes at the aa level.