A total of 5715 samples from several locations in Bangladesh were tested for AIV. The results of the virus isolation and rRT-PCR detection methods on identical samples were in agreement for 86.4% of the samples. Comparing the percent positive between the 2 methods is not meaningful because rRT-PCR was used to test 84.1% of the samples, yet virus isolation was performed on most of the suspected positives and only a small portion of negatives. Therefore, the percentage of infection as identified by virus isolation will be higher than expected if all of the samples had been tested by this method.