Of note, among 29 infants aged 0-6 months, two (6.9%) were positive for HBoV1, 2, and 3 IgG, and one (3.4%) was positive for HBoV4. The detection of HBoVs IgG antibodies among children aged 0-6 months may be due to the presence of maternal antibodies. However, the seropositive rate of HBoV1 was higher than that of HBoV2, 3, or 4 in the age groups of 0.5-2 years, 2-5 years, and 5-14 years (x 2 = 10.1,P,0.01; x 2 = 9.3, P,0.01; x 2 = 8.42,P,0.05, respectively). Based on the results of the cELISA, there were 38 (15.6%) individuals positive for HBoV1, nine (3.7%) for HBoV2, and two (0.8%) for HBoV3 among positive serum samples against single HBoV species. Notably, the two samples positive for HBoV4 IgG were also positive for HBoV1, 2, and 3 IgG.