Weight loss and temperature changes were not observed for any animals beyond expected minor daily fluctuations. Aged and juvenile mice exposed by the subcutaneous route remained clinically well during the period of observation and at euthanasia there was no evidence of HeV infection by histology, immunohistochemistry or qPCR (Table 1) . Specific binding antibody to the soluble form of the HeV G glycoprotein (HeV sG) was detected in sera of two juvenile animals only, using a Luminex microsphere assay [22] . Low levels of serum neutralizing antibody was also detected in one of these animals by serum neutralisation test (SNT) ( Table 2) .