LAMP reaction requires a constant temperature of about 60u-65uC for 60 min for amplification of DNA [27] [28] [29] [30] [31] [32] [33] [34] . In a previous study a pocket warmer reached 58uC in 30 min and stayed around 60uC for more than 60 min in a Styrofoam box [34] . The 3 pocket warmers (of a pack of 30 hand warmers) tested in this study achieved a temperature of 60uC after 60 min and maintained this temperature for about 90 min. The pocket warmer thus provided a suitable temperature (60uC) and time range (60 min) for amplification. Both pwLAMP and the conventional LAMP assays were able to detect to the limit of 300 copies of the target sequence after 60 min of amplification. This limit improved to 30 copies when the conventional LAMP was carried out at 65uC (the pocket warmer was not able to attain this temperature and was therefore not investigated). 