To operate the isolation chamber, the main power on the control cabinet is first turned on. The current operating parameters are displayed on the touch screen interface and can then be adjusted by the human operator by following the interface prompts. The isolation chamber should be monitored for 48 h to ensure that it is running normally. This includes supplying filtered air to the isolation chamber and ensuring that the exhaust air is cleaned by the double in-line HEPA filters and passed through the exhaust air system into the open air. Fresh air exchanges should be conducted at a rate of about 36 times per hour. Following 48 h of monitoring, the inside temperature is 22-23 °C, the relative humidity is 60%, the working negative pressure in the isolation chamber is adjusted to −100 Pa with respect to the laboratory. Appropriate anesthesia should be used on healthy medium-sized animals to pass them through the quarantine system and into the isolation chamber via the DPTE system. The animal experiment should be performed according to bio-safety operation standard procedures while the animal is still under the appropriate anesthesia. If the gloves are removed during the operation, a low pressure audible/visual alarm system is activated, and a minimum velocity value of 0.65 m/s in the center of the glove port is maintained. If a glove is damaged by a needle, the blowers are capable of maintaining the isolation chamber pressure at −100 Pa, but the alarm system would not be activated because the micro-differential pressure sensor is not sensitive enough for a leak of this size. Proper procedure dictates that the small hole be labeled by the operator and a new glove exchanged. All of the feeds, experimental material, waste, feces, and other materials can be transferred into or out of the isolation chamber by the DPTE system. There are several isolation chambers in one room, and the autoclave does not connect with any of them. Instead, DPTE β canisters are filled with items and are then sterilized in the autoclave. After sterilization, the sterile items are removed after opening the β door with specialized tools. The sterile items are then sent to a centralized disposal center for medical waste. Following the completion of studies with single animals, each animal is treated as dictated by bio-safety operation standards and general animal welfare. The cadavers are autoclaved in β canisters and sent to animal carcass disposal sites. The isolation chamber is connected to the peracetic acid sterilizer to sterilize its interior.