P. pastoris cultivation in shake flasks with methanol feeding without (A, B) or with (C, D) manual pH adjustment Figure 3 P. pastoris cultivation in shake flasks with methanol feeding without (A, B) or with (C, D) manual pH adjustment. Cultivations were performed in two phases: initial batch phase in BMG-medium and fed-batch in BMM-medium. Dissolved oxygen (pO 2 ) and pH were measured with a wireless measuring system, cell growth was followed by measurement of the OD 600 . Vertical lines represent methanol feeding points in A and C or start of methanol feed in B and D.