Each animal in turn was restrained either by hand or in an animal crush and four IRT measurements were taken of each of the animal's feet from different aspects (front, back, lateral and medial) and a measurement was also taken of the left eye (see Figure 1 for an example). These two sites were selected because, as mentioned above, researchers working on FMD had detected an increase in temperature around the coronary band and it is hypothesised that temperatures around the eye provide a non-invasive indicator of an animal's core temperature. Other sites commonly affected by FMD lesions such as the mouth and udder are less accessible and/or only applicable to lactating animals. To investigate the link between eye temperature and body temperature each animal's rectal temperature was taken at the same time as the thermal images using a digital thermometer. The IRT images were taken twice within ten minutes from each animal to evaluate repeatability of the measurements and correct for minor variations in the angle of the imager to the animal. Ambient temperatures were measured with a Fisher Scientific model FB70357 digital thermometer.