Since eEF1Bc was found in association with the TBSV repRNA in the cytosolic fraction of yeast, it is possible that eEF1Bc might affect the viral RNA recruitment from the cytosol into replication that takes place on the peroxisomal or ER membrane surfaces [42, 43] . Therefore, we tested the recruitment of the TBSV (+)repRNA to the membrane fraction in our CFE assay [23] . We found that eEF1Bc did not facilitate the association of the TBSV (+)repRNA with the membrane when applied in the absence of p33/p92 replication proteins ( Figure S2 ). Moreover, eEF1Bc did not further increase the amount of TBSV (+)repRNA bound to the membrane in the presence of p33/p92 replication proteins, which are needed for RNA recruitment ( Figure S2 , lanes 3-4 and 8-10) [24] . Therefore, we conclude that eEF1Bc is unlikely to promote the recruitment of the TBSV (+)repRNA to the membrane.