Reaction tubes were incubated at 60uC for 60 min in the heat block (GeneAmp 9700, Applied Biosystems, Foster City, CA) while with the pwLAMP, the tubes were sandwiched in a twofold pocket warmer (Hokaron Haru-type, Lotte Health Products, Tokyo, Japan) surrounded by a paper towel, and put in a Styrofoam box for 120 min (60 min reaction incubation). The reaction was terminated at 85uC for 5 min and the results were read by eye in ambient light and also using UV illumination. 