Objective: In the world literature, little has been published on the molecular pathways in primary uveal melanoma or subsequent development of metastases. The PI3K pathway has been implicated in regulation of apoptosis, cell cycle regulation, transcription and translation. Method: We used immunohistochemistry with TMA to investigate expression of PI3K, AKT and Phos-AKT, and PT in over 200 formalin fixed paraffin embedded blocks of uveal melanoma from the A.C. Camargo Hospital, São Paulo, Brazil from 1988 to 2005 . FISH was performed for PTEN copy numbers.