Resazurin-based chemistries continue to offer significant value for HTS because the reagents are robust and stable, well-validated, and easy to use. The single greatest attribute however, is that resazurin reagents generate amongst the most cost-effective data on a per well basis [21] . The major disadvantages of employing the chemistry are that the reagent is subject to interferences from compounds with inherent reductive capacity (such as ascorbic acid, glutathione, coenzyme A, dithiothreitol etc.) or those with intrinsic fluorescence characteristics [22] [23] [24] . Lastly, extended reagent incubations (2hr or longer) may produce toxic affects or augment affects produced from the experimental compound [25] .