We report 2 cases of invasive infection with SAFV type 2 in children. For each child, SAFV was detected in at least 1 compartment other than the central nervous system. This fi nding strengthens the evidence of an acute infection as the cause of clinical disease. In child 1, SAFV virus was found in the CSF and a fecal sample. In child 2, fi ndings were even more convincing because SAFV was found in 3 invasively collected samples: CSF, myocardium, and blood. No other credible cause of infection was found for either of the 2 children. In child 1, the only other positive fi nding was parechovirus type 3 in a fecal sample taken 2 weeks after onset of disease. Parechovirus was not found in the CSF and therefore seems unlikely as the cause of the acute symptoms. From child 2, several types of bacteria were identifi ed, but these seem unlikely to be the cause of death because culture of postmortem samples often grows commensal organisms. The bacteria from this child were not found consistently in the tissue samples, thus making systemic bacterial infection less likely. Verotoxinproducing E. coli also seems an unlikely cause of death because no diarrhea or signs of hemolytic uremic syndrome were present before the child's death or at autopsy.