In order to investigate the mechanism of suppression and desuppression of astrocyte differentiation-related genes in NTera-2 cells, we investigated and quantified differentially phosphorylated proteins using a newly developed label-free quantitation technol-ogy platform [30] . Briefly, phosphoproteins were digested in an optimized gel-assisted digestion and purified by a highly specific and reproducible automatic IMAC nanotechnology, followed by LC-MS/MS analysis. The relative quantification method was performed by software ''IDEAL-Q'' which was developed by SEMI alignment approach (Fig. 3A) .