Profiles were collected in the range 1-200 kDa at the two different ion focus mass 10 kDa ("low focus mass") and 50 kDa ("high focus mass"). The instrument was calibrated for dataset collection using all-in-one peptide standard (Bio-Rad Laboratories) in the 1-20 kDa range for 10 kDa low ion focus mass and all-in-one protein standard in the 20-200 kDa range for 50 kDa high ion focus mass [Additional file 3: Figure S1G ].