C. kelleyi were collected August 18, 2005, from a house in Jones County, Iowa, built in 1857. Bats had been excluded from the attic since 1992. Nine months before ticks were collected, bats were prevented from roosting under the eaves. DNA was extracted from 31 nymphal C. kelleyi, as described previously (6) . For each tick, regions of the glpQ, fl aB, and 16S rRNA genes were amplifi ed and sequenced as described (3, 7, 8) . Sequences were assembled by using the SeqMan program in the Lasergene software package (DNASTAR, Madison, WI, USA).