To assess the neutralising activity of antibodies induced by vaccination, we first set out to investigate if VLP entry into human hepatocyte cell line Huh7 could be inhibited. Pre-incubation of FITC-labelled VLPs (VLP-FITC) with PBS or naïve serum resulted in minimal inhibition of VLP entry ( Figure 5A ). However, the presence of an antibody against CD81, a cell surface molecule implicated in HCV entry into hepatocytes [30] , was able to prevent VLP entry into these cells by .90% confirming that these VLPs also utilise this molecule to facilitate cell entry. We next analysed the ability of sera obtained from mice inoculated with VLPs to inhibit the binding and entry of VLPs into Huh 7 cells ( Figure 5B ). Neutralisation of binding of VLPs to Huh7 cells was significantly greater in sera obtained from mice inoculated with VLPs in E 8 Pam 2 Cys (,50%) compared to sera obtained from mice inoculated with VLPs administered in Alhydrogel (,30%) or in saline (,20%).