Currently, the FMD diagnosis in our country (India) is being carried out using techniques developed at Project Directorate on FMD (PD FMD), Mukteswar which includes Virus isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (mPCR) and recently DIVA test which is an indirect ELISA for detection of antibody against nonstructural proteins. LPBE is being used for the detection of antibody titers against the FMD vaccinated animals. The S-ELISA is being used for the antigen detection using the material from the lesions but because of its low sensitivity currently mPCR is being used. The real-time PCR-based detection method is used in the many reference laboratories in the world for the purpose [7] .