Although the pocket warmers used in this study reached 60uC after 1 hr (instead of 30 min in previous studies [34] ), both devices achieved the requisite temperature and holding time for executing LAMP reaction, a major advantage in the use of amplification based assay for the detection of an infectious agent under field condition. The pwLAMP did not cross-react with other mycobacteria ( Figure 1) . Moreover, the experiment on clinical specimens demonstrated that the pwLAMP had a 100% specificity in clinical specimens of BU.