HBoV1 is primarily, although not exclusively [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] , a respiratory virus. We show here a higher prevalence of HBoV2 (particularly HBoV2B) than HBoV1 in stool samples from children and adults of different countries. A recent study testing for HBoV1 and HBoV2 DNA with use of species-specific nested PCRs failed to detect any HBoV2 in 16500 respiratory secretion samples from Edinburgh and Bangkok, whereas HBoV1 was found in 3% and 14% of these respiratory samples, respectively [43] . Another study found 5 (2%) of 212 nasopharyngeal samples from Korean children with acute lower respiratory track infections to be positive for HBoV2 DNA, but unexpectedly, no HBoV1 DNA was found [44] . Analyzing for HBoV1 and HBoV2 in both respiratory secretion and stool samples collected from the same individuals will be required to confirm whether the tropism of HBoV2 favors the digestive track and is distinct from that of the largely respiratory HBoV1.