The profile of IL-10 responses by CBA was very similar to that of IFN-c and TNF-a ( Figure S4 ) for cultures stimulated with IVTT products purified using NI-NTA resin ( Figure S4A ) or MagneHis Ni-particles ( Figure S4B ) with the best responses detected at 1:100 ( Figure S4 ). However, for unpurified or beadassociated IVTT products, the IL-10 profile was inverse to that of IFN-c and TNF-a with the best responses at a 1:100 dilution ( Figures S4C-E) , consistent with potential immunosuppressive effects of IL-10. Significant response was found only in EBNA3A 1:100 purified with MagneHis Ni-particles. As noted for TNF-a, the overall level of IL-10 was enhanced by association to Polybeads and Protein G beads relative to unpurified IVTT products (up to 7-fold with EBNA3A-Polybeads at 1:100 dilution). Responses for other cytokines assayed by CBA were not significant (data not presented).