PCR and TSE oligonucleotides were manufactured inhouse with a 48-column DNA Synthesizer (Northwest Engineering; now the 3900 from Applied Biosystems Inc.), using standard ␤-cyanoethyl phosphoramidite chemistry (for sequence information, see Tables 1, 2, and 3 in the Data Supplement accompanying the online version of this article at http://www.clinchem.org/content/vol50/ issue11/). Synthesis of sequences that contain iC and iG can also be purchased from IDT, Eurogentec, or EraGen. A demonstration assay encompassing this specific system can be purchased from EraGen Biosciences. Amino-labeled EraCode oligonucleotides were manufactured with an ABI 394 DNA Synthesizer (Applied Biosystems) using 3Ј-PT-Amino-Modifier C6 CPG (Glen Research). Target-specific extender primers were manufactured with the spacer phosphoramidite C3 (Glen Research) placed between the EraCode complement and the target-specific region. The isoG and isoC phosphora-