Conclusion People with PEMT gene G175A SNP were more susceptible to develop NAFLD Aim: 1) Determine whether there is a relationship between NAFLD, Protein C and Paraoxonase levels in quiescent and in regenerating rats fatty liver 2) determine the effect of ISA on hepatic "protein C" and paraoxonase mRNA. PE439 Methods: Forty-eight SD rats were treated with fructose enriched diet (FED), or FED with Metformin (2 mg/Kg/d), FED with Rosiglitazone (3 mg/Kg/d), or the combination of both drugs for 5 wks. 30% PHX was performed at WK 5. Protein C, paraoxonase mRNA expressions, lipids, MDA were measured before and 24 hours after PHX. Results: Hepatic "protein C" mRNA was higher in rats with fatty liver than control rats (+105%, p<0.01) whereas hepatic paraoxonase mRNA was lower in rats with fatty liver than control rats (-28%, p<0.005). Hepatic protein C and paraoxonase mRNA increased in rats with fatty liver in regeneration (+116%, p< 0.01, and +15%, p<0.01 respectively). The combination of metformin and rosiglitazone decreased hepatic protein C expression at 24 hours after PHX by -170% (p<0.001) and increase paraoxonase mRNA by +50% (p<0.01). Serum paraoxonase correlates with serum protein C (r=-0.2), MDA (r=0.4), Background: Non-alcoholic steatohepatitis (NASH) is a type of non-alcoholic fatty liver disease (NAFLD), and may progress to hepatic fibrosis and cirrhosis. The pathogenesis of NASH remains unclear. The aim of this study was to explore the arginase change in the progress of steatohepatitis in rats. Methods: Male SD rats weighing 70-80g were obtained. Twenty animals were randomly divided into two groups. In the model group, five animals were fed with high lipid forage that includes 3% cholesterol and 20% lard for 6 weeks, five were fed for 12 weeks, while the control group ate normal foods. The animals were sacrificed after 6 weeks. The animals were sacrificed after 6 weeks and 12 weeks. Liver and blood serum were collected while the serum levels of ALT, AST, TG and TC were measured. The pathology of liver was observed by HE staining. Western blot was used to investigate the expression of arginase in control and model group.