In 2 of 681 CSF specimens (n = 333 and n = 348 from cohorts 1 and 3, respectively) from children with meningitis (online Appendix Table, wwwnc.cdc.gov/EID/ article/17/12/11-1037-TA1.htm), hCV RNA was detected at low concentrations (1.14 × 10 4 and 9.63 × 10 2 copies/ mL). In 1 of these patients, hCV was also detectable in feces (9.50 × 10 2 copies/g). In 1 of 10 myocardium specimens, hCV was detected by nested RT-PCR, and results of quantitative real-time RT-PCR were negative. Underquantifi cation because of nucleotide mismatches below oligonucleotide binding sites and contamination of nested RT-PCR was excluded by sequence comparison (up to 5% nt divergence from other hCV strains, including the positive control). Serum and liver specimens from the patient who died of SIDS were negative according to realtime RT-PCR. No histopathologic alterations could be observed in myocardial tissue from this same patient.