In nature, TBFV establish and maintain a persistent infection across the various life-stages of the arthropod host [8] . In an effort to model and study this aspect of TBFV infection in vitro, ISE6 cells were infected with LGTV, serially passaged at confluence, and examined at intervals for the induction and maintenance of a persistent infection. Interestingly, cultures of infected ISE6 cells appeared normal for a period of at least one year following initial infection.