Comparison between SILAC and 2D Gel Analysis-Comparing 2D gel analysis and SILAC approaches revealed that the SILAC method provided more information. However, only one protein (hnRNPA2/B1) was identified by both methods, and the depletion of eIF6 and RBM4 was not detected by SILAC. This reflects two key points: first, the lower sensitivity of the 2D gel approach, and second, that for the SILAC analysis, we only divided the one-dimensional gel electrophoresis sample into six slices prior to analysis.