Determination of the Surface Coverage of Immobilized 7-, 6-, and 5-Repeat Forms of DC-SIGNR. The amount of DC-SIGNR immobilized on the supported NTA-TRIG-DLGE monolayers was quantified by surface plasmon resonance. 21 For these measurements, the LangmuirÀBlodgett monolayer of NTA-TRIG-DLGE (65 Å 2 /lipid) was supported on a monolayer of 1-octadecanethiol that was self-assembled on a gold-coated glass slide. The sample was then assembled in the surface plasmon resonance cell, while maintaining it under solution at all times.