All the 93 HBoV-positive cases detected by conventional PCR were also confirmed by real-time PCR performed us-KJLM ing the TaqMan probe. The viral load detected in the HBoVpositive samples by using real-time PCR was in the range of 1.3 × 10 3 -4.6 × 10 9 copies/mL (median, 1.82 × 10 5 copies/ mL). The HBoV-positive cases were categorized in 2 groups: low-viral-load group (viral load ≤ 1.0 × 10 6 copies/mL, N = 58) and high-viral-load group (viral load > 1.0 × 10 6 copies/ mL, N = 35) ( Table 1 ). The HBoV-positive patients aged less than 3 yr had a significantly higher viral load than that in the patients aged more than 3 yr (P = 0. 001).