Various bioactive constituents are expected to be present in T. esculentum plant. These may include phenolic constituents, carbohydrates, and certain fatty acids, among many components. Large amounts of gallic acid have been detected in T. esculentum plant [3] . Gallic acid esterifies with glucose, the resultant hydrolysable tannins (HTs), secondary metabolites widely distributed in the plant kingdom, are known to be effective antagonists against viruses [3] . Tylosema esculentum tubers contain yet to be determined phenolics [3] . High amounts of phytosterols have also been detected in T. esculentum oil (about 75% of all phytosterols being 4-desmethylsterols and about 15.72% of the total being 4,4-dimethylsterols) [4] . Rotavirus (RV) infections are a major cause of acute gastroenteritis in infants and young children, accounting for about 611,000 deaths each year worldwide, mostly in developing countries [5] . Rotavirus is also an important pathogen in many agricultural species, inducing serious diarrhoeal diseases in neonatal and postweaning pigs and calves [6] [7] [8] . Rotavirus infection is limited to the mature enterocytes of the tips of the intestinal villi of humans, domestic animals, and mice, leading to severe gastroenteritis in the young [9] . Some strains of bovine and human RV have high sequence homology and interspecific infectivity, both implying phylogenetic relatedness [10, 11] . Previous in vitro tests of the bovine RF strain of RV on human, pig, chicken, and goat intestine epithelial cells in our laboratory have shown interspecific infectivity of rotavirus [12] , and, therefore, the bovine strain of rotavirus was used to infect human and pig intestinal cells in the current study.