For the mixture GIA experiment, 4 mg/ml of non-AMA1(3D7) or non-AMA1(FVO) IgGs were mixed with US-total IgG. The concentration of US-total IgG was determined at which the IgG was expected to give ,60% inhibition in the final mixture in the standard GIA. The growth-inhibitory activity of mixtures was determined using the standard GIA.