RNA was extracted from urinary pellets and subjected to realtime PCR for ACE2. ACE2 mRNA was detected in 45% of urine samples of transplant patients, with no significant difference between diabetic [0.669 (IQR, 0.00-6.15) pg mRNA/mg Cr610 25 ] and non-diabetic subjects [0.00 (IQR, 0.00-2.06) pg mRNA/mg Cr610 25 ; p = 0.091].