Some studies have shown palmitoylated lipopeptide constructs to elicit long-lived, protective cellular responses against a variety of pathogens, including Hepatitis B virus (HBV), influenza virus, and Plasmodium falciparum [25] [26] [27] [28] [29] . Our work herein shows that mice immunized with mixture of CD8 + and CD4 + eliciting peptides and lipid Pam 2 Cys emulsified in GLA-SE elicited higher IFN-γ production than mice immunized with lipopeptides constructed with the same components of CD4 + and CD8 + eliciting peptides, and Pam 2 Cys. The approach using cocktails of non-covalently linked lipid mixed to helper T lymphocytes(HTL) and CD8 + T cell (cytolytic T lymphcyte[CTL] and IFN-γ eliciting) epitopes for simultaneous induction of multiple CD8 + T specificities would have significant advantages in terms of ease of vaccine development.