Our results show moderate expression of the luciferase reporter gene. Use of a CMV promoter in the construction of the SV40 vector may improve transgene expression. However, low level of proteins expression may not be entirely disadvantageous. Supra-physiological production of specific proteins may lead to unexpected side effects. In contrast, low expression may be augmented by repeated administrations of the vector [40] . With the SV40 vector RT-PCR for luciferase mRNA detection Figure 6 RT-PCR for luciferase mRNA detection: High levels of mRNA were detected in the lungs of 2CLP animals (plasmid pGL3 used as positive control for luciferase). Minimal levels were present in the liver, kidney spleen and none in the heart. Minimal expression in the lungs, kidney, liver, spleen, and heart of T0 and SO animals. GAPDH was used as a house keeping control gene, using PCR primers specific for GAPDH.