Based on the genomic sequence information we designed gene-specific primers for the amplification of full length CEACAM1-related cDNAs from total RNA, isolated either from liver or spleen. In the published genomic dog sequence, one nucleotide is missing from the A1 exon of canine CEACAM1. Cloning and sequencing of full length CEACAM1 clones from a mix-breed dog revealed a complete 279 bp CEACAM1 A1 exon. In total, we could iden- Expansion of ITAM-bearing CEACAM1-related CEA family members in dog No leader exon could be identified for CEACAM23 because of a sequence gap (indicated by brackets) in the publicly available genomic sequences. The genes are arranged in the order and orientation as found on dog chromosome 1.