We used a VLP-based ELISA to assess the seroprevalence of HBoV1-4 in healthy individuals in Beijing, China, ranging from 0 to 70 years old. Our results show that the seroprevalences of HBoV1, 2, and 3 range from 40.3-67.8% in children 0.5-2 years old and are up to 100% in adults. To eliminate the interference of antibody cross-reactivity, the results were corrected with a cE-LISA. The results of our cELISA suggest that the seroprevalences of HBoVs are significantly lower than those obtained without competition, especially in adults. These findings further indicate a high degree of antigenic cross-reactivity between HBoV1-4. Our findings are consistent with those of a recent study of Finish and Pakistani individuals, in which the seroprevalences of HBoVs decreased after depletion of heterologous HBoV reactive antibodies [22] .