With the advent of microfabrication technology, one of the directions taken to address the future needs of bioanalysis and clinical diagnosis is the development of micro total analysis systems (µTAS) or labs-on-a-chip (LOC). This scaling down capability supports an exceptional ability to miniaturize various functional units such as pumps and reactors, making it possible to integrate and automate processes into a microsystem. Additionally, it offers important advantages over bulk or large-scale analysis including rapid assay results, high-throughput screening, and low consumption of reagents. Further, the energy required for microfabrication and operation is remarkably reduced. Most importantly, these benefits make microchip systems amenable to near-patient and point-of-care testing. The development of DNA amplification microinstruments began in the 1990s, when the concepts of integrated microfluidic devices were introduced to take advantage of microfabrication technology for biological and chemical analyses [2] . To establish such a system, it was desirable to create a totally integrated device performing a series of specific molecular functions such as nucleic acid extraction and purification, nucleic acid amplification and detection, and other supporting analysis techniques, with minimal dead volumes.