In summary, consistent with the results following in vitro stimulation with IVTT products, robust antigen-specific IFN-c responses could be induced by in vivo boosting with PyCSP IVTT products purified by either NI-NTA resin or MagneHis Niparticles, but not by unpurified IVTT reactions or IVTT products associated to Polybeads or ProteinG beads. There was no significant difference between purification with either NI-NTA resin or MagneHis Ni-particle, nor between purified IVTT products and plasmid DNA. The magnitude of Th1 (IFN-c and TNF-a) responses stimulated in vitro by purified IVTT PyCSP products were similar to those stimulated by PyCSP plasmid DNA.