The coding region for the CedPV N protein was amplified by PCR with a pair of primers flanked by AscI (59 end) and NotI (39 end) sites for cloning into our previously described GST-fusion expression vector [47] . The expression and purification by gel elution was conducted as previously described [48] . For antibody production, purified protein was injected subcutaneously into 4 different sites of 2 adult (at a dose of 100 mg per animal) New Zealand white female rabbits at days 0 and 27. The CSIRO's triple adjuvant [49] was used for the immunization. Animals were checked for specific antibodies after days 5 and 42 and euthanized at day 69 for the final blood collection.