Anti-sense oligonucleotides have been shown to be effectively used as therapeutic agents against viral infection. In one such study siRNA generated against the cd loop-coding sequence in domain II of the viral Envelope protein (which is highly conserved among all flaviviruses because of its essential role in membrane fusion) has been found to protect against lethal encephalitis [10] . Similarly siRNAs has also been generated against various nonstructural proteins of JEV and were found to be effective in inhibiting viral replication [11, 12] . Anti-sense approach has also been employed to inhibit flaviviral replication by generating anti-sense molecules against RNA elements within the 59 and 39 UTRs in flaviviral genome. In one such approach, DNAzyme against 39 UTR of JEV genome has be found to be effective in controlling virus infection in a murine model [13] . Under the same approach but with different kind of anti-sense oligonuleuotide called Morpholino, flaviviral replication has been inhibited in cultured cells as well as in animal models [14, 15] .