The mixtures were incubated horizontally in a shaker (200 rpm) at 60uC overnight. To capture the DNA, 40 ml of diatomaceous earth solution (10 g diatomaceous earth obtained from Sigma Aldrich Chemi GmbH in 50 ml of H 2 O containing 500 ml of 37% (wt/vol) HCl) was added to the suspensions and incubated at 37uC with shaking (200 rpm) for 60 min. The mixtures were centrifuged at 14,000 rpm for 10 sec and the resulting pellets were washed twice with 900 ml of 70% ethanol (2-8uC) followed by 900 ml of acetone. The pellets were dried at 50uC for 20 min and resuspended in 100 ml milli Q purified water and centrifuged at 14,000 rpm for 10 sec. The purified DNA was used as templates for both IS2404 PCR and LAMP assays to detect M. ulcerans.