Placing the identified mutations in the three novel gain-offunction mutants of eEF1A (V 301 D, L 374 V/N 377 K, and F 413 L; Fig. 1C , indicated with yellow balls), which exhibited increased tombusvirus replication, over the known structure of eEF1A [51] revealed a cluster on one face of eEF1A (namely, the actin bundling domain III), away from the domains known to bind to tRNA and translation factor eEF1Ba. On the other hand, the new reduced function mutant (A 76 V, Fig. 1C , indicated with green balls) and the previously identified T 22 S [25] , which exhibited