Descriptive statistics were generated to examine the distribution of melanoma-reactive B cell cultures from each patient including the mean, 95% confidence interval and maximum reactivity to melanoma cells. A two-sided Student's t test was used to compare the mean reactivity of antibody cultures derived from melanoma patients to healthy volunteers to primary or metastatic melanoma cell lines and to compare antibody responses between patients with non-metastatic and metastatic disease. A one-way ANOVA was used to compare antibody reactivity to a metastatic melanoma cell line among B cell cultures derived from patients with stage II, III and IV disease with a Tukey's post hoc comparison test. A twosided Student's t test was used to compare antibody-mediated tumor cell killing between tumor-specific and non-specific monoclonal antibodies derived from the same patient. A twosided Student's t test was also employed to compare the movement of immune effector cells, pre-incubated with antibodies, in contact with tumor cells to the movement of immune cells not in contact with tumor cells. All statistical analyses were performed using GraphPad Prism software (version 5.03, GraphPad, San Diego, CA) and error bars in all figures represent 95% confidence intervals. Video S1 Real-time live-cell cytotoxicity assay for the 6_2G3 melanoma-specific antibody. (AVI) Video S2 Real-time live-cell cytotoxicity assay for the 6_2D10 non-melanoma-specific antibody (negative control).