The cytokines IL1B, IL6, IL8, TNF and IL12 were quantified using commercial ELISA tests (DuoSet ELISA development kits, R&D Systems, USA). For quantification of basal levels of cytokines in supernatants from mock-stimulated cells, the samples were not diluted for quantification. Supernatants collected from stimulated cells were diluted (1:22 for IL1B and IL8, 1:1 for IL6, 1:10 for TNF and 1:2 for IL12). All samples were tested in duplicates. Absorbance was read at 450 nm using an ELISA plate reader (MRX revelation, Dynex). Results were expressed as pg of cytokine/mL.