To test whether endogenous STING undergoes the same NS2B3-dependent processing as in overexpression experiments in 293T cells, we infected human MDDCs with DENV-2 (16681 strain) and analyzed the cell lysates by western blot at different time points ( Figure 1H ). Infection of MDDCs by DENV resulted in the degradation of STING that could be detected at 24 and 48 hours post infection (hpi) ( Figure 1H , lanes 9 and 10) which correlate with peak expression levels of the NS2B3 (as detected with NS3 specific antibodies). As expected, this degradation of STING was not observed in MDDCs treated with UV-inactivated DENV or mock treated cells ( Figure 1H , lanes1-5 and 11-15). These data demonstrate that DENV NS2B3-dependent cleavage of endogenous human STING occurs in cells relevant to DENV infection (MDDCs), and therefore has the potential to play a crucial role in inhibition of type I IFN production.