For experiments with DN-dynamin-2 or DN-Eps15, uptake of transferrin and virus was normalised to the results obtained for cells expressing the control (wt dynamin-2 or the Eps15 control respectively). Over-expression of wt AP180 inhibits CME and cannot be used as a control for AP180C [6] . Therefore, for experiments with AP180C, transferrin or virus uptake was normalised to the results obtained for cells of the non-expressing population (i.e. the non-green cells). Similarly, for DN-dynamin-2 or AP180C, infection was normalised to the level of infection of cells expressing wt dynamin-2 or cells of the non-expressing population, respectively. The level of infection of inhibitor-treated cells was normalised to the levels of infection of the mock-treated controls. Student's t test was used to determine statistical significance.