Virions were purified using the mMACS Streptavidin Kit (Miltenyi Biotec Inc., Auburn, CA.), according to the manufacturer's instructions using biotinylated-L243 (Biolegend). GHOST assay [82] to determine infectious virus production and p24 ELISAs to determine virus particle release using the HIV-1 p24 CA Antigen Capture Assay Kit were performed following the manufacturer's instructions (NIH AIDS Research and Reference Reagent Program).