For subject 185F, V1V5 domain backbone, forward primer 59-Phos-atgagggacaattggagaagtg-39 (HXB2 nt 7647 to 7668) and reverse primer 59-Phos-gctttaagctttgatcccataaac-39 (HXB2 nt 6576 to 6553); V1V2 domain backbone, forward primer 59-Phoscaagcctgtccaaaggtctct-39 (HXB2 nt 6831 to 6851) and reverse primer 59-Phos-gctttaagctttgatcccataaac-39 (HXB2 nt 6576 to 6553); V3V5 domain backbone, forward primer 59-Phos-atgagggacaattggagaagtg-39 (HXB2 nt 7647 to 7668) and reverse primer 59-Phos-ccttacacacacaatttctac-39 (HXB2 nt 7118 to 7098); ectodomain backbone, forward primer 59-Phos-aagatatttataatgatagta-39 (HXB2 nt 8271 to 8291) and reverse primer 59-Phostttttctctctccaccactctcc-39 (HXB2 nt 7754 to 7732); V5 domain backbone, forward primer 59-Phos-atgagggacaattggagaagtg-39 (HXB2 nt 7647 to 7668) and reverse primer 59-Phos-catgttatgtttcctgcaatg-39 (HXB2 nt 4527 to 4507).