Samples of 5610 5 isolated cell populations were infected with DENV-2, 16681 at the indicated MOI in a total volume of 100 ml of DC media for 1 hour at 37 C. Then, DC media supplemented with 4% HS was added up to a final concentration of 10 6 cells/ml and cells were incubated for the remainder of the infections at 37 C. At the indicated times, cell supernatants were collected and cell pellets were used for RNA extractions. Whole PBMCs were used after ficoll centrifugation and samples of 60610 6 PBMCs were infected with DENV-2 at MOI of 1 or left uninfected. After 1 hour DC media 4% HS was added. Eighteen hpi supernatants were collected and isolation of the different cell populations after DENV-2 infection was carried out as described above.