PBC obtained a relatively higher average error rate of 0.169% and 237 ambiguous calls per sample (2855 in total). Our results showed that EvolSTAR is robust and performs well when samples are prepared under sub-optimal conditions. Even for nasal swab samples that tend to have much less concentration of virus RNA than cell cultures, EvolSTAR suffered only a slight drop in performance compared to Nimblescan PBC. In conclusion, we have shown that EvolSTAR is robust and generates sequence calls of high accuracy and reproducibility in this pilot study consisting of 40 microarray experiments. Meanwhile, efforts will be put in to continually evaluate EvolSTAR with more samples and update it on a regular basis as the H1N1(2009) influenza virus evolves.