The following experiment was performed to determine antibody response after immunization with Ad vectors. Female BALB/c (H-2K d ) mice at 6-8 weeks of age were obtained from the Jackson Laboratory (Bar Harbor, ME). Groups of at least eight mice were analyzed in each experiment or at each time point. Ads were injected into each group of mice: Ad5, Ad5/HVR2-MPER-L15(Gag), Ad5/HVR2-MPER-L15DE1, AdCMVGag, Ad-CMVEnv at 1610 10 VP per mouse using intramuscular (i.m.) injection. These mice were boosted 14 days after prime with 1610 10 VP of the same vector. For CD8 T cell response analysis, blood was collected from mice vaccinated with Ad5/HVR2-MPER-L15(Gag) or AdCMVGag. All animal protocols were approved by the Institutional Animal Care and Use Committee at the University of Alabama at Birmingham.