HLA-A03 supertype CD8 + T cell epitopes included: KSFKDILPK (SAG1 224-232 ), AMLTAFFLR (GRA6 164-172 ), and RSFKDLLKK (GRA7 134-142 ). PADRE (AKF-VAAWTLKAAA) was the universal CD4 helper peptide used in vaccine constructs. Pam 2 Cys (Pam 2 -KSS) also was included. Lipopeptide constructs used in this study are shown in Figure 1 . Peptides and lipopeptides were synthesized by Synthetic Biomolecules, San Diego at > 90% purity. Additional HLA-A03 supertype bound peptides and their initial grouping into pools for in vitro studies are shown in Tables 1 and 2 . A TLR4 agonist, a GLA-SE adjuvant, was synthesized by the Infectious Diseases Research Institute (Seattle, Washington) as a stable oil-in-water emulsion. AMLTAFFLR (GRA6 164-172 ) and additional new peptides were first dissolved in DMSO and then diluted in PBS.