Methods: Morning urine and 24 hour urine were collected from 96 patients over three years age who were hospitalized in a tertiary pediatric liver service. Each patient was re-evaluated according to WD Scoring system, and was assigned to one of the three groups: WD, suspecting WD, and non-WD. 24, 6, and 64 cases were assigned to WD, suspecting WD, and non-WD respectively. Urine copper and zinc concentration was determined simultaneously by using Inductively Coupled Plasma Mass Spectrometry.