The osteoclast-like cells generated on Osteologic® calcium phosphate-coated culture wells were shown to be capable of resorbing the immediate calcium phosphate layer surrounding them. That result was in direct contrast to precursor RAW 264.7 cells (cultured in the absence of M-CSF and RANKL) that were shown not to affect the calcium phosphate coating in any manner. Osteoclast-like cell cultures treated with the mAb-sCT conjugate, or mAb alone, demonstrated a significant inhibitory effect on the resorptive ability of the osteoclast-like cells, as measured by the conservation of calcium phosphate layer on the Osteologic® slide. The integrated density value (IDV) of the remaining calcium phosphate layer for the culture treated with the mAb-sCT conjugate was significantly greater than that treated with sCT alone (Fig. 8 ). Protein was transferred to nitrocellulose membrane which was blocked with 5% skim milk, followed by the incubation with anti-RANK mAb. Reaction was detected by using GAM-HRPO, and the membrane was developed using ECL reagent and exposure to X-ray film. 