For the crude preparations however, it is noteworthy that the detection rate of the LAMP assay was significantly higher for the unboiled extracts than for the boiled extracts. Explanations for these results were not explored. The observation that the LAMP assay was not inhibited especially for the unboiled specimens is quite consistent with previous work that have shown LAMP to be tolerant to culture medium and to certain biological substances including phosphate buffered saline, serum, plasma, urine and vitreous [32] .