The SPR experiments were carried out at 25 °C by using a repeated angle mode and a fixed angle mode. The repeated mode is the method for measuring the changes of the minimum resonance angle in specially fixed angle range by repetitive angle scanning and fitting, which is a plot of change in the reflectance intensity as a function of time (Resonance vs. Time). SPRLAB™ (K-MAC, Korea) system detects a refractive index and thickness change containing semiconductor laser source with 635 nm wavelength and dielectric silicon photodiode detector, which has incident angle range of 30-80 degree.