In this study, the chimeric CGMMV vectors pRT, pRG, and pCG-FSRTRE were constructed by inserting the EB4 coding sequence to the end of the CGMMV CP ORF in plasmid pCGT7X. The maps of these constructed clones are shown in Figure 1 , which indicates their respective position of the primers during amplification and cloning procedures. Maps of plasmids pCGT7X are carrying the wild-type CGMMV, and pCANTAB 5E are carrying EB4 with their respective priming sites are also as indicated in Figure 1 . The genome size of wild-type CGMMV is approximately 6.4 kb (without the plasmid backbone), and the resulting chimeric CGMMV genome would be approximately 6.5 kb in size and contains the EB4 and readthrough sequences as well as inserted HindIII restriction recognition sites and additional nucleotides enabling inframe cloning. The pRT and pRG chimeric clones were constructed with the read-through sequence of TMV (CAA-UAG-CAA-UUA). This leaky sequence meets the minimal sequence requirement for effective read-through of the stop codon [12] and had been used successfully in previous reports [14] .