On the basis of newly acquired sequence information, specific qPCR assays were designed (Table 1) to screen pooled organ tissues of all 120 bats. Cycler conditions for all qPCR assays were as follows: predenaturation (95uC for 10 min), 45 amplification cycles (95uC for 30 s, 60uC for 30 s, 72uC for 30 s), and final extension (72uC for 10 min).