In vivo peptide blocking C57BL/6 mice were infected i.p. with 10 4 Pruigniund tachyzoites. At day 21, 23, 25, and 27 post infection the animals were injected i.p. with either 0.5 ml a-CXCL10 (0.5 mg/mL), 0.5 mL a-CXCR3 (polyclonal), or 0.5 ml PBS as previously published [76, 91] . The mice were sacrificed on day 28 p.i. and brains were excised for flow cytometric analysis, and parasite burden as described above. Blocking studies were conducted twice with at least 5 biological replicates.