A nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV) was developed. Two detection methods, NAS-BAelectrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzymelinked oligonucleotide capture (NASBA-EOC), were evaluated and compared with other laboratory-based methods, data analysis support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV [223] .