Virus was harvested and processed for HA N-glycosylation pattern analysis according to Schwarzer et al. [2] applying an optimized work-flow [3] and data evaluation [4] . Finally, the samples were separated by CGE-LIF using an ABI PRISM 3100-Avant genetic analyzer (Applied Biosystems, Foster City, California, USA). For data processing and evaluation the x-axis of capillary electropherograms was normalized using an internal standard, resulting in N-glycosylation patterns, in which each peak corresponds to at least one distinct N-glycan structure. This allowed a direct qualitative comparison regarding N-glycan structure presence in different samples. For quantitative comparison, the relative peak height (RPH: the ratio of peak height to the total height of all peaks) was determined for each peak and sample. Low abundant peaks were defined with RPH < 5 %.