To test the impact of pooling peptide candidates, LSP longD12, longD1 and longD8-9 were mixed in equal parts (mixW) and applied at the same conditions as described above. The cut-off was determined by the mean A 405nm of fifty blood donor samples plus 2 standard deviations. Sensitivity and cross-reactive behavior was tested with 30 echinococcosis sera and 15 sera from various helminth and amoeba infections that had previously shown crossreactivity with the single peptides.