We selected fecal samples from 479 children <5 years of age with gastroenteritis that had been submitted for viral diagnostic testing from September 2009 through February 2010 and tested them for SAFV. Nucleic acid extracted from these samples was combined into 48 pools, with 9 or 10 samples per pool. Samples from pools with positive results were identifi ed, and new extractions from these pools were tested individually. However, enough sample material for new extractions was available for only about half of the samples.