To identify the primary fraction containing viral induced membranes that included the viral replication complex, we carried out a comparison of the viral RNA genome copies to labeled lipid in the 16K and CE subcellular fractions ( Figure 4C ). The results indicated that the 16K membrane fraction was enriched (a ,3fold increase) in viral RNA (compared to CE) at the 36 hr time point while at the 60 hr time point the CE fraction had slightly more viral RNA per labeled lipid (compared to the 16K fraction).