Practically, the genome of interest is interrogated from a defined four-base pair position, classically the NlaIII site. An anchoring enzyme binds to this defined complementary position and acquires adjacent short mRNA sequences (9-10 base pairs) that, based on the first assumption earlier, allows specific mapping of these transcripts to genomic libraries. Proportionate amplification of differ-ent transcripts gives a realistic quantification of gene expression for the particular experiment or disease condition (67, 103) .