S.K. Mohan 1 , S. Subramaniam 2 , S. Subramaniam 3 1 Assistant Professor, Department of Biochemistry, Saveetha Medical College & Hospital, Saveetha University, T.N, INDIA., 2 Consultant, Department of Biochemistry, Apollo Hospitals, Chennai, T.N, INDIA. , 3 Department of Biochemistry, Apollo First Med Hospitals, Chennai, T.N, INDIA. Background: Non-Alcoholic Fatty Liver Disease (NAFLD) covers a spectrum of liver diseases from simple fatty infiltration to progressive fibrosis. Non-Alcoholic Steato Hepatitis (NASH) is a severe form of NAFLD and progresses to the end stage of liver disease. It is becoming the leading cause for referral to liver clinics in most areas. The prevalence of NAFLD in Indian population is estimated around 7 -13%. The NAFLD has the potential to progress to hepatocellular carcinoma or liver failure, both events that ultimately lead to early death. Aim: To evaluate the combination of Inter Cellular Adhesion Molecule -1 (ICAM -1), Adiponectin and Type-IV collagen, a new biomarker profile for NASH in patients with NAFLD. Methods: 76 patients with NAFLD and age & sex matched 68 normal healthy individuals as controls were selected for this study. Levels of Serum ICAM -1, Adiponectin, Type-IV collagen, lipid profile and liver function test parameters were estimated in patients and compared with controls. Results: Serum ICAM -1 & Type -IV collagen levels were significantly increased in patients with NASH among the NAFLD patients compared to controls. The Serum Adiponectin levels were significantly reduced in patients with NASH among the NAFLD patients compared to controls. Compared to liver function test parameters and lipid profile levels, NASH profile has got positive negative predictive value among the NAFLD patients. Conclusion: In patients with NAFLD, NASH profile Test -a simple, noninvasive and reliable to predict the presence or absence of NASH. Background/Aim: Oxidative stress and cytokines plays an important role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Aim of study was to assess lipid peroxidation, serum levels of transforming growth factor-( TGF-) and tumor necrosis factor-( TNF-) in patients with NAFLD and compare it with patients of chronic viral hepatitis (CVH) and healthy controls (HC). Methods: Lipid peroxidation was studied by estimating plasma malondialdehyde (MDA) levels as per the methodology described by Buege and Aust and TGF-& TNF-levels were measured by ELISA kits (Ray Biotech, USA, & Diaclone, UK) in the stored sera in 10 biopsy proven patients with NAFLD (M: 4, F: 6, Mean age: 41.7 ± 11.0 yrs), 15 patients with CVH ( M:14, F:1, Mean age: 33.7 ± 11.4 yrs) and 5 HC (M:5, Mean age: 25.2 ± 2.7 yrs). Results: There was no difference in mean plasma MDA levels amongst patients with NAFLD (17.28 ± 3.6 mol/L), CVH (15.29 ± 2.3 mol/L) and HC (16.79 ± 1.2 mol/L). Serum TGF-levels between NAFLD (0.56 ± 0.41 ng/mL) and CVH (0.52 ± 0.25 ng/mL) patients and HC (0.58 ± 0.57 ng/mL) were also comparable. Though patients with CVH (17.2 ± 27.0 pg/mL) and NAFLD (7.5 ± 6.3 pg/mL) had higher levels of TNF-than HC (5.5 ± 1.1 pg/mL), the difference was not significant statistically. Conclusion: Lipid peroxidation, TGF-and TNF-need to be studied in a larger number of patients with NAFLD. Background/Aim: Burnt out nonalcoholic fatty liver disease (NAFLD) may be responsible for cirrhosis and hepatocellular carcinoma (HCC) in the absence of other causes. Aim of this study was to evaluate the surrogate markers of NAFLD in patients with cryptogenic cirrhosis (CC) and cryptogenic HCC (CHCC). Methods: Sixty five patients with CC and 31 patients with CHCC were analyzed for the presence of abnormal body mass index (BMI) and type 2 diabetes mellitus (DM Aim: To investigate the relation of phosphatidylethanolamine N-methyltransferase PEMT gene G175A single nucleotide polymorphism (SNP) with the susceptibility to nonalcoholic fatty liver disease NAFLD . Methods The genotypes and allele frequencies of PEMT exon 8 SNP G175A were analyzed by using PCR-RFLP in 51 NAFLD patients and 50 controls.