To validate 'entry' constructs by sequencing, ViralORFeome automatically aligns uploaded sequence traces onto virtual clones using either an extended Libalign Perl tool or Phred and T-Coffee programs that build first a contig of sequence traces before the alignment is performed (23, 24) . Validated 'entry' constructs can be subsequently used to recombine viral ORFs into various Gateway Õ -compatible destination vectors to achieve protein expression. ViralORFeome allows users to keep track of the different expression constructs that have been established for each viral ORF.