Quantification of eosinophil tissue infiltration within each biopsy using EPX-mAb immunohistochemistry was independently performed in an intra/inter-blinded fashion that included investigators from pathology (KL, EJ, and PG -EPX-mAb based eosinophil counts in lung tissue); KL and EJ -EPX-mAb assessments of eosinophil degranulation), a hospital/clinic-based pulmonary fellow (KP -EPX-mAb based eosinophil counts in lung tissue), and a PhD graduate research fellow (LW -EPX-mAb based eosinophil counts and degranulation in lung tissue). All evaluations were done at a magnification of 400x. The number of positively stained eosinophils was determined in the alveolar lung parenchyma as a numerical average calculated from 10 randomly selected hpf; that is, a total area of~3 mm 2 per biopsy-investigator. The reported values are the mean ± SEM of investigator-derived counts.