RT-PCR using broadly reactive degenerate primers for a 185 nt fragment in the 3D-like RNA-dependent RNA polymerase (RdRp) region of the calicivirus ORF1 gene was positive in two specimens. Repeated screening using more sensitive specific primers revealed three additional positive specimens. Further information on the species and RT-PCR screening results are presented in Table 1, Table S1 and Figure S1 . Sequence similarity search using BLASTN against the NCBI nonredundant nucleotide database did not reveal significant similarity to known SaV sequences. Another search using BLASTX against the NCBI non-redundant protein database produced hits to SaV sequences, with the most closely related sequence being the RdRp sequence of porcine SaV (GenBank accession number ACT98315) at 43% aa identity. A phylogenetic tree was constructed from the nucleotide alignment based on the length of the partial RdRp sequence obtained from bat SaV/TLC72 (GenBank accession number JQ267527) ( Figure S2 ).