Sequencing was carried out on RT-PCR-amplified products of viral RNA extracted from putative chimeric virus particles at 30 days postinoculation (dpi) and total plant RNA isolated from inoculated plant materials at 14 and 21 dpi to confirm the expression of EB4. Sequence analysis was done using BioEdit Sequence Alignment Editor Software (version 6.0.5) (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). The chimeric CP carried the complete EB4 sequence within its genome at 14 dpi ( Figure 6 ). However, the EB4 sequence appeared to be truncated at 21 dpi with an upstream portion of the EB4 sequence was not present. Only part of the downstream sequence of EB4 was detected together with the 3 untranslated region of the CGMMV. EB4 was totally undetectable at 30 dpi. Interestingly, the 5 end of introduced read-through sequence (position 490 to 501) was retained in the genome. The introduced HindIII recognition site at EB4 downstream from position 639 to 644 was found to have been deleted after 30 dpi. Even though only a single band was visible on RT-PCR screening, it was suspected that there could be products with similar sizes which could not be separated in normal agarose gel electrophoresis, therefore, the RT-PCR products from the chimeric CGMMV RNA at 30 dpi were cloned into pGEM-T Easy vector and subjected to sequence analyses. The results (Figure 7 ) confirmed the absence of the EB4 sequence, except for clone pR P3U4 (from nucleotide position of 702 to 746), where only part of the downstream sequence of the EB4 was detected together with the 3 untranslated region of the CGMMV. The 5 end of introduced read-through sequence of AAA-TAG-CAA-TTA (position 594 to 605) was retained within the genome for all sequenced clones (Figure 7) . The introduced HindIII site from position 742 to 747 was deleted for clones pR P3U1, pR P3U3, and pR P3U11 after 30 dpi.