To more precisely evaluate the relative distribution of the proteins during infection, we co-stained specimens for multiple viral proteins. In each case, the bulk of NS3 staining was colocalized with both the viral membrane (prM) (data not shown) and envelope (E) proteins (Fig. 3A) ; although the overlap appeared less complete in the ISE6 cells. NS5 was diffusely distributed in the cytoplasm, but there was clear evidence that the staining for E overlapped (Fig. 3B) . The punctate staining pattern for NS1 also exhibited coincidence with prM, E, and NS3 but with fewer areas of colocalization with NS5 (data not shown) in both Vero and ISE6 cells. There were no appreciable differences between the acute and persistently infected ISE6 cells (Fig. 3) .