Honey bee samples were collected during their travels from Mississippi through South Dakota to California resulting in a prospectively collected 10-month time-course of 431 data points, each consisting of 50-100 bees isolated separately from both the entrance (older foragers) and brood comb (younger house bees). Hives # 10, # 14 and # 19 were lost in December due to queen death or infertility. We analyzed all the entrance samples (5 bees per colony each time-point) using the APM.