The dominant trait that attracted the most attention for plastid transformation has been herbicide tolerance [28, 35, 56, 57] . Roudsari et al. [28] revealed the production of high level glyphosate tolerant plants (N. tabacum) through biolistic transformation of plastids by introduction of a mutated herbicide-tolerant gene coding for EPSP synthase. Plastid transformation is routine, however, only in tobacco and the efficiency of transformation is much higher in tobacco than in other plants [58] . Lee et al. [50] discussed the major obstacles to the extension of plastid transformation technology to other crop plants which includes regeneration via somatic embryogenesis.