In summary, we have shown that CGMMV has a readthrough codon preference and that the read-through codon for TMV was shown to be not efficient, as the chimeric CGMMV transcripts utilizing this signal were not infectious. The reported limitation of low-modified coat protein yield of this type of read-through transient expression system appears to have been overcome as relatively equal yield of chimeric and wild-type CGMMV coat protein were produced. This report also provides a rational harvesting timeline for the chimeric virus making this system exploitable for implementation in a plantation scale in the future. It can be suggested that once host plants are infected with the chimeric virus carrying the inserted foreign peptides, the optimum harvesting time would be at around 14 dpi or not more than 20 dpi in order to obtain maximum yield of the full-length transgene. Growth of the infected plants for longer periods to obtain higher yields of the chimeric virus may induce unwanted transgene deletions. This and other factors described earlier should be relevant information for the further development of CGMMV or other plant viruses as vectors for medically important peptides such as for dengue (this study) and Hepatitis B [7] viral antigens. Enhanced Hygiene Measures and Norovirus Transmission during an Outbreak Control of norovirus outbreaks relies on enhanced hygiene measures, such as handwashing, surface cleaning, using disposable paper towels, and using separate toilets for sick and well persons. However, little is known about their effectiveness in limiting further spread of norovirus infections. We analyzed norovirus outbreaks in 7 camps at an international scouting jamboree in the Netherlands during 2004. Implementation of hygiene measures coincided with an 84.8% (95% predictive interval 81.2%–86.6%) reduction in reproduction number. This reduction was unexpectedly large but still below the reduction needed to contain a norovirus outbreak. Even more stringent control measures are required to break the chain of transmission of norovirus. The estimated maximum likelihood estimates are α = 3.35 and β = 1.09, resulting in a peak generation time of 2.6, and a mean generation time of 3.6 days. Other positively skewed unimodal distributions such as the Weibull distributions did not produce a significantly better fit.