For the spike dataset two image populations were combined composed of side, and end-on perspectives. For the side view perspective images were subfield directly off of the Ebola virus cryo-EM images. For the end-on perspectives sub-tomographic volumes were extracted from the tomographic reconstructions of the Ebola VLP. The 3D volumes were then added in the ''Z'' plane to generate 2D projection averages which were then used for the subsequent single particle image analysis. The data were the processed using EMAN to generate an initial 3D reconstruction, which was then refined in SPIDER using the projection matching technique as previously described [44, 56] . The docking of the 3CSY.pdb [42] structure to the cryo-EM structure of the spike was accomplished using the SITUS [57] software package with the exception that only the GP1 and GP2 components of the 3CSY structure were used for the docking process. The ''floodfill'' program in SITUS was used to segment the spike component of the 3D cryo-EM reconstruction from the envelope component of the reconstruction. The segmented volume was then used for the docking procedure using the 'colores' function in SITUS. Once docked the entire 3CSY.pdb structure which included the Fab of the KZ52 neutralizing antibody was superposed over the docked GP1/GP2 component of the structure.