Termination-reinitiation in yeast cells was studied using the yeast dual-reporter plasmid pAC99 [22, 23] . The wild-type termination-reinitiation signal of BM2 was prepared by PCR as an EcoRV fragment that was subsequently cloned into MscIdigested pAC99. pAC99-BM2 derivative plasmids were generated by site directed mutagenesis as described below.