To address if these arrays can be used as a practical, large-scale re-sequencing tool, we have developed a system comprising customized sequence amplification primers, a 12-plex DNA resequencing array and an automated base-calling and variant analysis software (EvolSTAR). We demonstrate that the sequences obtained from the array are highly reproducible with !99.99% accuracy and 99.02 ± 0.82% genome coverage. The short turnaround time from sample to sequence and analysis results ($30 h for 24 samples) makes this kit an efficient large-scale evolutionary surveillance tool.