A range of methods have been used for isolation of immunoglobulins from colostrum or milk. These include traditional methods of ammonium sulfate precipitation and column chromatography [3, 5, 145, 184] . Affinity chromatographic methods used to isolate IgG include lectins [185] ; protein A or G chromatography [186, 187] , and more recently, isolation with protein A/G immobilized electrospun polyethersulfone membranes [188] ; metal chelate chromatography [189, 190] ; and adsorption with polyanhydride microparticles [191] . The range of detection and quantification methods for IgG, most often analyzed by radial-immunodiffusion [192] or enzyme-linked immunosorbant methods [193] , are now expanding to include methods that detect multiple proteins, such as thermally addressed immunosorbant assays [194] , and rapid methods that may be integrated into milking systems, such as surface plasmon resonance-based immunosensors [195] .