After the light emission measurement, at 48 hrs, the animals were sacrificed and internal organs harvested. One lung was removed, preserved in formalin, embedded in paraffin, cut at 5 μm thickness and stained with hematoxylin and eosin. H&E sections were evaluated for lung injury, degree of injury and its distribution within the lungs [14] . Part of the spleen, liver, heart and one kidney were also harvested and prepared in the same way. The remaining part of the spleen, heart, liver, the second kidney and lung were frozen in fluid nitrogen and preserved at -80°C for RNA.