The antimicrobial activities of the extracts were determined by the Kirby-Bauer agar diffusion method according to NCCLS standards [37, 38] . Sterilized molten agar (20 ml) was dispensed to each sterile disposable Petri dish (diameter 9 cm) and allowed to solidify. Mueller Hinton agar was used for bacteria and Sabouraud dextrose agar for yeast. Microbial suspension (200 μl) containing approximately 3 × 10 6 CFU was spread evenly onto the surface of the solidified medium. The plates were allowed to dry for 15 minutes before the test discs were placed at equidistance from each other. Each plate consisted of one standard antibiotic disc and three other discs impregnated with various extracts.