Objective: Studies show that there is association between salvia officinalis extract administration and liver or heart functions. The main aim of this study was to determine the effects of salvia officinalis extract on serum level of alkaline phosphatase in male rats. Method: Male wistar rats were randomly divided into control, normal saline receiving and salvia officinalis extract (100, 150 or 200 mg/kg/day) receiving animals of 5 in each group. After a period of 6 weeks, blood samples were collected using cardiac puncture method. Following serum collection, serum alkaline phosphatase levels were measured by spectrophotometry method. Data were statistically analyzed and compared between groups using ANOVA.