Expression levels of interleukin (IL) 1a, IL-2, IL-4, IL-6, IL-10, tumor necrosis factor (TNF) a, interferon (IFN) c, macrophage inflammatory proteins (MIP)-1a, MIP-1b, monocyte chemotactic protein (MCP)-1, MCP-3, and Regulated upon Activation, Normal T-cell Expressed, and Secreted (RANTES) in the lung homogenates were quantitatively determined by flow cytometrybased immunoassay (Mouse Th1/Th2 cytokine 10plex and Mouse Chemokines 6plex Flowcytomix Multiplex, Bender MedSystems) according to the manufacturer's protocol. Interleukin 1b and keratinocyte chemoattractant (KC) simplex were purchased separately (Bender MedSystems). In brief, lung homogenates were prepared and processed accordingly. The samples were acquired on a BD LSRII (BD Bioscience), and the amount of cytokine (ng/mL) was calculated by FlowCytomix Pro 2.3 software (Bender MedSystems).