Shown in Figure 2B , three probesets in GDS1076 dataset, 1419114_at, 1419115_at and 1419116_at, were mapped to the mouse gene Alg14 (GeneID: 66789), with the former two corresponding to IPI00132168 and the latter one corresponding to IPI00405947. Both proteins were indicated in IPI as homologs of yeast asparagine-linked glycosylation 14 without any further information. We notice two interesting phenomena in this case. First, the two probesets correlating to a same protein do not show similar expression profiles. Instead, they behave like the probesets correlating to different variants characterized by a PCC value of 0.4080 (P = 0.0415). This issue might be due to some factors causing microarray hybridization efficiency shift. Secondly, according to our calculation, the expressions of these two variants are negatively correlated with PCC values of -0.7740 (P = 5.04e-5) for 1419116_at and 1419115_at, and -0.4402 (P = 0.0296) for 1419116_at and 1419114_at. So far there are no reports on expression regulation of these two transcript variants of gene Alg14, and no function reports of the corresponding protein isoforms. This negative correlation suggested that these two proteins might perform different roles thus should be distinctly annotated.