In this study we have explored the potential of NiV virus-like particles (VLPs) as a vaccine. Plasmid-mediated expression of selected viral proteins results in the spontaneous assembly and release of VLPs. These particles make highly effective immunogens because they possess several features of the authentic virus such as their surface structure and dimensions [31, 36] . They are also safe because they do not contain any viral genetic material. VLPs, where one or more of the constituent proteins serve as immunogens (native VLPs), are particularly effective as vaccines for infectious disease. The fact that two such vaccines [Gardasil (Merck & Co) for human papillomavirus (HPV), and Sci-B-Vac (SciGen) and Bio-HepB (GlaxoSimthKline) for Hepatitis B virus (HBV)] have already been approved for human use, and many, for non-enveloped and enveloped viruses [31, 32, 37, 38, 39, 40, 41, 42] are at various stages of development, attests to the desirability of this approach for vaccine development.