Phospholipase A2 activity was monitored using a Red/Green BODIPY PC-A2 substrate (Invitrogen) using a similar method as described in [31] . C6/36 cells were infected with DENV at an MOI = 3 in 6-well plates. Following virus adsorption, the cells were overlayed with MEM (supplemented as above) and 10% heat inactivated fetal calf serum (2 ml/well). At the indicated time points, the media were removed, and new media (1 ml/well) containing 6 mM of the fluorogenic phospholipase A substrate (BODIPY-PC) were added to the cells. The cells were incubated at 30uC for 30 min. Following incubation, media was removed and the cells were washed with 16PBS. The lipids were then extracted using butanol-1 (1 ml/well). The aqueous fraction was discarded and the lipids in the butanol phase were analyzed by mass spectrometry to monitor the conversion of BODIPY-PC to BODIPY LCP by PLA 2 .