We have previously reported that dengue reactive memory B cells are common following both primary and secondary DENV infection [30] . To further characterize the human B cell response in donor 033, we immortalized memory B cells. PBMCs were isolated and IgG+ memory B cells were immortalized with EBV and CpG as previously described [34] . The immortalized B cell culture supernatants were screened for antibodies that bound to C6/36 insect cells infected with DENV3. Thirty five percent of the B cell cultures generated from donor 033 were positive following this initial screen (Table 2) . From the dengue positive cultures only 7.5% of the cultures bound to rE protein (Table 2) . To further characterize the binding and functional properties of human antibodies, we isolated hMAbs from donor 033. Isolation and characterization of DENV-specific hMAbs from donor 033