We next evaluated the activity of non-AMA1 IgGs from Malian children by GIA at two different concentrations in GIA well using homologous strain of parasites (i.e., non-AMA1(3D7) IgGs were tested against 3D7 strain of parasites and non-AMA1(FVO) IgG with FVO parasites). The % inhibition of non-AMA1 IgG was plotted against that of the original total IgG pool ( Figure 2 ). The slope of the best-fit line is 0.97 (95%CI: 0.81-1.14) for the 3D7 data set and 0.87 (95%CI: 0.64-1.10) for FVO. Since the slope was not significantly different from 1, it showed that the depletion of AMA1-specific IgGs from the total IgG did not materially change growth-inhibitory activity, even though AMA1-specific IgGs from the same total IgG pools also showed activity (Figure 1 ).