RdRp with the (+)RNA template ( Figure 2B , lanes 3-4) or eEF1Bc with the TCV RdRp ( Figure 2B , lanes 7-8) prior to the RdRp assay did not result in increase in (-)RNA synthesis. Overall, data shown in Figure 2B imply that eEF1Bc can stimulate (-)RNA synthesis only when eEF1Bc binds to the (+)RNA template before the RdRp binding to the template.