The spleens of rZH501-M847-A-infected mice (Fig. 6E ), ZH501-infected mice (Fig. 6D ) and mice inoculated with the mixture of rZH501-M847-G and rZH501-M847-A (data not shown) were characterized by a depletion of lymphocytes in the white pulp from day 2 p.i. to day 5 or 6 p.i., with maximum All infected mice, except for rZH501-M847-G-infected mice, had moderate, non-suppurative encephalitis throughout their brains from day 5 p.i. (Fig. S2A ). Viral antigens were detected as early as day 2 p.i. in the endothelial cells of some mice. After day 5 p.i., viral antigens were detected in neurons at the thalamus, and spread to neurons of the surrounding areas (Fig. S2A, inset) .