The intra-assay and inter-assay CV of this established FQ-PCR was in the range of 1-3% for most of the dynamic range (from 1.0 × 10 9 to 1.0 × 10 2 pAHV-1 plasmid copies/ μL), but increased to more than 6% at viral DNA loads lower than 1.0 × 10 2 pAHV-1 plasmid copies/μL and increased to more than 4% at viral DNA loads more than 1.0 × 10 9 pAHV-1 plasmid copies/μL ( Table 1 ). The results Figure 1 Establishment of the fluorescent quantitative real-time PCR standard curve. Standard curve of the AHV-1 fluorescent quantitative real-time PCR. Ten-fold dilutions of standard DNA ranging from 1.0 × 10 9 to 1.0 × 10 2 copies/μL were used, as indicated in the x-axis, whereas the corresponding cycle threshold (CT) values are presented on the y-axis. Each dot represents the result of triplicate amplification of each dilution. The correlation coefficient and the slope value of the regression curve were calculated and are indicated. demonstrated that the established fluorescent quantitative real-time PCR method was characterized by a wide dynamic range (8 logarithmic decades) of detection from 1.0 × 10 9 to 1.0 × 10 2 pAHV-1 plasmid copies/μL with high precision. However, at lower and higher dilutions quantitation was not always reproducible compared to other properly diluted samples. Therefore the dynamic range of the method was between 1.0 × 10 9 and 1.0 × 10 2 pAHV-1 plasmid copies/μL, which is relatively broad.