Bohle Iridovirus (BIV) is a ranavirus in the family Iridoviridae. BIV was originally isolated from the Bohle River region in northern Queensland [9] and is the only documented isolation of a virus from amphibians in Australia. It is capable of infecting cane toad tadpoles [10] and is therefore a candidate for testing the viral delivery of genes in this species. Furthermore, in recent studies we have shown that BIV can carry and express foreign genes in vitro [11] .