Bioinformatics analysis of Illumina deep sequencing-determined transcriptome of primary Tupaia hepatocytes De novo reconstruction of transcriptome from cDNA library deep sequencing data Total 253,919,616-pair 72 nt sequences with 36.6G base from the sequencing results of the hepatocyte cDNA library described above were fed to Trinity (Grabherr et al., 2011) r20110519 using pair end RNA-seq protocol, with which 209,063 transcripts of average length of 1421 nt (minimum 300 nt, maximum 21,043 nt, and scaffold N50 of 3674 nt) were generated.