In July 2000, 38 macaques within the Sangeh monkey temple area and surrounding forest were darted opportunistically and sedated with 3 mg/kg of Telazol (tiletamine HCl/zolazepam HCl). Following universal precautions, researchers withdrew 10 mL of blood from each macaque's femoral vein. The macaques were closely monitored during anesthesia and recovery. Six milliliters of blood was placed in a serum separator tube and centrifuged in the field to extract the serum. The remaining blood was placed in a tube containing EDTA. Sera and whole blood were frozen and stored at -20°C.