The newly designed multi-functional isolation chamber system reported here achieves multiple technical improvements: (1) By using variable frequency drive blowers as the inlet air and extraction blowers, adjustments for rapid pressure changes (e.g., insertion of gloves) can occur automatically without breaching the inert atmosphere. The extraction blowers contain an integrated backup system with one blower running at full strength and another on standby to act as a backup. Negative or positive pressure state is kept at a stable and safe level through the automatic control system. The pressure is adjusted depending on different requirements for different animals and/or experimental conditions. (2) A DPTE 270 α door mounted on one side wall of the isolation chamber allows for the transfer of experimental materials. Another DPTE 270 α door is mounted on the bottom of the isolation chamber near the disinfection pool with a slightly tilted bottom. Animal excrement can be discharged to the DPTE container via the flexible silicone diversion tube. The excrement collection package is then minified. An experimental sample can also be transferred to the bio-safety cabinet through a shorter DPTE container. (3) The control cabinet installation is comprised mainly of the PLC, electric element (which includes the voltage transformer, secure alternating current contactor, circuit breaker, electric cable, indicating lamp, and button), printer port (for data output), and industrial Ethernet interface (which allows for the remote data control of multiple isolation chambers by the WINCC 6.0 program system). Automatic control and monitoring of the isolation chamber and sterilization system are achieved by the exchange of data between the touch screen and control cabinet through the industrial bus Profibus DP to meet different laboratory animal research project parameter requirements such as pressure, humidity, temperature, illumination, and disinfection. A human operator can set the isolation chamber environment parameters according to the requirements of the infectious animals or for cleaning animals, allowing for the acquisition of adequate and authentic data. (4) Animal welfare is ensured by installing adjustable illumination lamps, a rotatable camera, a flat television, a micro-differential pressure sensor, and a temperature humidity sensor to maintain comfortable living conditions for the animal.