To determine whether JMN3-003 predisposes host cells against viral infection by inducing an antiviral state, we pre-treated cells with the compound, followed by wash-out of the substance and virus infection after different time periods. Independent of incubation time after removal of the compound, we could not detect any substantial inhibitory effect in this set-up (figure 6C), arguing against priming of the innate antiviral response by JMN3-003. Likewise, preincubation of viral particles with JMN3-003 prior to removal of the article and infection lacked any appreciable antiviral effect (figure 6D), excluding direct virucidal activity of the substance.