The persistently infected tick cells also contained round vesicles enclosed within dilated membrane structures (Fig. 8C and Movie S3). The 3D analysis confirmed that the elongated profiles were in fact tubular, and that they were clustered in fascicle-like groups surrounded by a single membrane. These structures were cylindrical in shape with closed ends, varying in width from 60 to 100 nm and in length from 100 to 800 nm. Pores were seen between tubules and vesicles ( Fig. 9C & F) ; however, in our examinations of numerous tubular structures, we were unable to identify pores linking tubules with other tubules or with the cytoplasm.