Vaccination with peptide pools including newly identified peptides and adjuvants elicits IFN-g and provides more protection to mice against Type II parasite challenge Then, HLA-A*1101 transgenic mice were immunized with peptide pools which included these newly identified peptides: KSFKDILPK (SAG1 224-232 ); AMLTAFFLR (GRA6 164-172 ); RSFKDLLKK (GRA7 134-142 ); STFWPCLLR (SAG2C 13-21 ); SSAYVFSVK (SPA 250-258 ); AVVSLLRLLK (SPA 89-98 ) plus PADRE and Pam 2 Cys in GLA-SE, They were immunized three times at intervals of two weeks. Significant IFN-γ spot formation responses were observed in vitro by the cells from immunized mice exposed to all the peptides except GRA7 134-142 . Figure 7a shows the representative data of IFN-γ spot formation from the four immunization groups which were stimulated by individual peptides. The inclusion of the newly identified peptides thus had potential to enhance immunogenicity in transgenic HLA-A*1101 mice.