For transmission electron microscopy (TEM), Vero or ISE6 cells were seeded on 13-mm Thermanox coverslips (Nunc) in 12 well plates at 1610 5 cells/well. After 24 hours, cells were infected with LGTV TP21 at an MOI of 10 and incubated for 48 hours at 37uC and 32uC, respectively. Cells were then washed twice with DPBS and fixed with 2.5% glutaraldehyde in cacodylate buffer (100 mM sodium cacodylate, 50 mM KCl, 2.5 mM CaCl 2 ) for 30 minutes at room temperature prior to overnight incubation at 4uC.