The OpenArray TM high-throughput, massively parallel real-time PCR platform (36) (BioTrove) was tested for compatibility with s-RT-MELT. Forty-eight samples of p53 exon 8 PCR products were generated from 48 different lung adenocarcinoma samples and mutation-containing cell lines and processed via the hybridization and enzymatic steps of s-RT-MELT. Real-time PCR in the OpenArray TM platform was performed with the LightCycler FastStart TM DNA Master SYBR Green TM I (Roche) using 0.2 mM M13f and 0.2 mM oligodT-anchor-mix as primers pre-positioned on the array through-holes (36) and polyA-tailed DNA as template. The cycling conditions were as follows: 1 cycle at 948C for 2 min, 25 cycles of 908C for 15 s, 558C for 20 s and 688C for 30 s for reading fluorescence using a high sensitivity imaging camera (36) . The real-time PCR step was immediately followed by real-time differential melting curve analysis. Raw data were exported in Excel software for further analysis. The OpenArray TM experiment was repeated twice at the company's headquarters.