In 1999, we proposed that all human MHC specificities should be mapped ("the Human MHC Project") as a preamble for the application of MHC information and technologies in humans (Buus 1999) . Since then, we have developed large-scale tools that are generally applicable towards this goal: production, analysis, prediction and validation of peptide-MHC interactions (Ferre et al. 2003; Harndahl et al. 2009; Hoof et al. 2009; Larsen et al. 2005; Lundegaard et al. 2008; Nielsen et al. 2003 Nielsen et al. , 2007 Ostergaard et al. 2001; Pedersen et al. 1995; Stranzl et al. 2010; Stryhn et al. 1996) , and a "one-pot, read-and-mix" HLA-I tetramer technology for specific T cell analysis (Leisner et al. 2008) . Here, we demonstrate that many of these tools can be transferred to other vertebrate animals as exemplified by an important livestock animal, the pig. We have successfully generated a recombinant swine leukocyte antigen I (SLA-I) protein, SLA-1*0401, one of the most common SLA molecules of swine (Smith et al. 2005) . Using this protein, we have developed the accompanying biochemical peptide-binding assays and demonstrated that the immunoinformatics tools originally developed to cover all HLA-I molecules, despite the evolutionary distance, can be applied to SLA-I molecules. We suggest that the "human MHC project" can be extended to cover other species of interest.