From the results of the two assays, we selected four target peptides, NP158 (GMD-PRMCSL), NP189 (MVMELIRMI), NP373 (AMDSNTLEL), and NP458 (FQGRGVFEL) for animal experimentation. Based on our findings, three of the peptides showed the highest binding affinity to the HLA-A2 molecules and they were able to activate the highest number of CD8+ T cells. Since NP158 (GMDPR-MCSL) exhibited a comparatively low ability to stimulate T cells against the peptide-loaded T2 cells and induced only a small amount of the IFN-c release, it was included in the sample peptides as a control for checking the consistency of the results previously shown by the in vitro T-cell stimulation experiment.