We have described a novel high-throughput platform that incorporates nucleic acid chemistries that simplify genetic analysis. PLx uses one additional base pair not found in nature in combination with microsphere flow cytometry technology to eliminate the most cumbersome steps commonly found in established multiplexed systems. The EraCode molecular recognition system described is particularly suited for the Luminex 100 instrumentation because the solid support can be dispensed into the complex reaction and decoding can be done at room temperature without washing, creating a true "Liquid Chip" type of system. Our study indicates that the chemistry is transferable to a clinical setting. In addition, although not required, the process can be performed with a robotic workstation for higher throughput.