Growth parameters (A) and concentrations of product-related mRNA species (B) during cultivation of a P. pastoris for produc-tion of recombinant human collagen II in shake flasks Figure 1 Growth parameters (A) and concentrations of product-related mRNA species (B) during cultivation of a P. pastoris for production of recombinant human collagen II in shake flasks. Cultivation procedures with two methanol pulses per day (control, blue open circles, interrupted blue line) and pO 2 -dependent manual feeding of methanol (red filled circles, red continuous line) were compared.