Objective: Proliferating trichilemmal cysts are relatively common occurring within the scalp of elderly women. Proliferating trichilemmal cysts of the breast, however, are rare. These cysts are benign with very few demonstrating malignant transformation. Method: We report a case of proliferating trichilemmal cyst of the breast with atypical cytology which prompted excisional biopsy. Cytological assessment was useful in this case as it indicated excisional biopsy rather than a more extensive excision. Results: Smears produced from the FNA showed sheets of atypical epithelial cells with foamy histiocytes, multinucleate cells and fibrotic material with evidence suggestive of fat necrosis. A cell block was acellular. A specific diagnosis was not made. An excision specimen was advised and a proliferating trichilemmal cyst was demonstrated. Objective: A large clinical value has determination of molecular subtype of breast cancer (BC). To clarify morphological and molecular biological similarities and differences between BC depending on age, we compared features between tumors of younger and older women. Method: 573 patients with BC are included in research: from 18 till 86 years, among them 254 patients were till 35 years. ER, PR, Her-2/neu, p53, p63, Ki67, CK5/14, p21, Bag1, Mcl1, pS2, VEGFR, Her-1 were analyzed in all cases by immunohistochemistry. Results: The younger patients with BC had higher expression of p53, p63, p21, Bag1, Mcl1, Ki-67, VEGFR, HER-1 (p<0,001) than older patients. There was a basal-like molecular subtype of BC 3-fold more frequent compared with older patients (21,6 % versus 7,2 %; p<0,0001). Threeyears overall survival in patients till 35 years was by 11,5 % and a five-year overall survival was by 15,5 % lower than in patients over 35 years (p<0,001). Objective: Breast HER2 ISH relies on correct enumeration of HER2/CEP 17 signal. However, as with immunohistochemistry, there are technical pitfalls which may render ISH difficult/unreliable to interpret. The UK NEQAS has established a 'technical' ISH module to provide feedback to laboratories using both chromogen and fluorescence based tehcniques. Method: Unstained slides consisting of 4 breast cancer cell lines were distributed. Laboratories were asked to demonstrate HER2 gene amplification using their routine assay, and then return the slides for assessment. Chromogen based methods were assessed around a multi-header microscope with 4 assessors scoring each slides, whilst the fluorescent ISH method was assessed by a single individual. Assessors scored the 'readability' of each slide, without counting the probes, and provided feedback where the hybridisation technique could hinder interpretation. Results: CISH based methods showed acceptable staining in 50 % (N=32) of cases. Staining problems ranged from loss/poor HER2/CEP17 signal, non-specific staining and morphological damage. The FISH pilot assessment showed an acceptable rate of 32 % (n=21), with the low pass rate being attributed to poor preservation/quenching of fluorescence signal.