Approximately 750 L1 stage worms were grown in a single well of a 48 well plate containing 150 mL S media [42] and E. coli OP50. When worms had reached the L4 to young adult stage, glucose was added to 100 mM and either 20 mM HEPES (pH 8.0) or Cry5B dissolved in 20 mM HEPES (pH 8.0) to give a final concentration of 100 mg/mL was added. After one hour, worms were removed, centrifuged, and 175 mL of media was removed. Twenty five mL of 26 sodium dodecyl sulfate loading buffer was added, and worms were boiled for 5 minutes. Ten microliters of lysate were used for immunoblotting. Monoclonal antibody to phospho P38 MAPK (Cell Signaling Technology cat. no. 9215) was used at 1:300 and monoclonal antibody to a-tubulin (Sigma-Aldrich cat. no. T6199) was used at 1:4000.