To determine the recombination activity of HCV-derived sequences, C.quinoa plants were inoculated with four BMV mutants: MatH-HVR-BMV, MatNH-HVR-BMV, MatH-X-BMV and MatNH-X-BMV. Their genomes were composed of wtRNA1, wtRNA2 and one of the newly generated Mat0-RNA3 derivatives (either MatH-HVR-, MatNH-HVR-, MatH-X-or MatNH-X-RNA3) ( Table 2) . After 2 weeks, the standard procedure of BMV RNA3 progeny analysis was applied. The number of lesions developed during each infection was counted. The 3 0 -portion of progeny RNA3 was amplified by the RT-PCR method. The length of RT-PCR products was established (by electrophoresis in a 1.5% agarose gel), then they were cloned and sequenced.