Enzymatic activities were measured from yeast lysates prepared from induced cultures. Measurements showed comparable levels of b-galactosidase in all the samples, indicating that P27 or replicase expression did not affect the stability of the dual reporter mRNA or the translatability of the first reporter ( Table 2 ). The effect of P27 or replicase expression was monitored by comparing the reporter activity ratios to those measured from cells harbouring the empty expression plasmids (Table 2) . Co-expression of CfMV replicase did not affect the normalized firefly luciferase expression (LUC/LacZ) from the inframe control, whereas slightly increased luciferase expression from the test construct was observed. In contrast, P27 expression reduced firefly luciferase expression both from the test and the inframe constructs. The effect was stronger in the presence of inframe control as normalized firefly luciferase levels reached only 54% of expression measured from the empty vector control.