Methods: Immunohistochemical analysis of tissue samples from 56 patients with chronic hepatitis B (CHB), 12 acute hepatitis B (AHB) patients and 10 health controls was performed. Results: PD-1 was positively expressed on lymphocytes infiltrating the portal area.PD-L1 expression was the same as PD-1,also expressed in interlobular.PD-L2 expressed on kupffer cells and dendritic cells.PD-1-,PD-L1-,and PD-L2-positive cells express index of CHB patients were much more than that of health controls and AHB patients(p 0.05).Between groups in CHB,the expression rate increase with the disease progression (p 0.05).