LAMP for Detection of Mycobacterium ulcerans www.plosntds.org PCR for IS2404 PCR targeting IS2404 was performed as described previously [21] . The first and second round PCRs used primers pGp1: 59-AGGGCAGCGCGGTGATACGG-39and pGp2: 59-CAGTG-GATTGGTGCCGATCGAG-39 and pGp3: 59-GGCGCAGAT-CAACTTCGCGGT-39 and pGp4: 59-CTGCGTGGTGCTTT-ACGCGC-39, respectively.