Ultra-pure E.coli lipopolysaccharide (LPS, 0111:B4 strain-TLR4 ligand, product number tlrl-pelps), pepinh-MyD (product number tlrl-pimyd) or pepinh-TRIF (product number tlrl-pitrif) inhibitory peptide (InvivoGen, San Diego, CA) were prepared as suggested by the supplier. Mouse thrombin and bovine serum albumin were purchased from Sigma Chemical Co., St. Louis, MO, USA. Cellular origin of antigens was determined using platelet (rat anti-mouse CD41 antibody) and total leukocyte (rat anti-mouse CD45 antibody) membrane specific fluorescein conjugated {Phycoerythrin (PE)-or fluorescein isothiocyanate (FITC)-} antibodies by flow cytometry. PE-or FITC-conjugated Annexin-V and matched isotype control antibodies were purchased from BD PharMingen International, San Diego, CA. All other reagents and solvents used in this study were of analytical/ reagent grade.