Development of an effective vaccine would prevent this disease. Attenuated T. gondii tachyzoites (e.g., S48, TS-4, T-203, ΔRPS13)have been employed for live vaccinations of non-human animals [10] [11] [12] [13] [14] [15] . Parasites attenuated by knockout of gene transcription recently have been proposed as a new type of attenuated vaccine candidate [13] [14] [15] . However, safety considerations may limit vaccination of humans with live organisms. Development of peptide-based vaccines created with epitopes which elicit IFN-γ production by CD8 + T cells [16] is a promising strategy to mobilize the immune system against T. gondii in humans [16] [17] [18] [19] [20] . Thus, an effort to create an immunosense epitope-based vaccine was initiated.