In tick cells, acute LGTV infection induced 3D rearrangements similar to those seen in Vero cells (Fig. 8B and Movie S2). The membrane proliferation, while not as marked as in Vero cells, still resulted in a continuous network that surrounded the vesicles. The majority of vesicles observed in the tick cells were round (Fig. 7C &  D) ; however, slightly elongated vesicles were seen more frequently than in Vero cells. Communicating pores or openings between the vesicles and the cytoplasm (Fig. 9B ) and between individual vesicles (Fig. 9E) were also seen in the tick cells.