Different populations of cells were isolated from human blood and subsequently infected with DENV-2 at MOI of 1 and 12 h after infection supernatants were collected and RNA was extracted from cells. DENV-2 RNA was detected in all cells tested including plasmacytoid DCs (pDCs), B cells, blood circulating DCs (cDCs), monocytes as well as in monocyte-derived DCs (MDDCs) ( Figure 8A ). We also analyzed the cytokine and chemokine expression profile in all those cells by qRT-PCR (data not shown) and by multiplex ELISA (Figure 8B ) in the supernatants at 12 hpi. We observed a marked chemokine response (IL-8 and MIP1b) in monocytes, MDDCs, B cells and cDCs at this early time point, ( Figure 8B ). However pDCs did not show any significant chemokine profile after DENV-2 infection at this time point ( Figure 8B ). More interestingly, there was no significant type I IFN production observed in any of the cells tested by qRT-PCR (data not shown) or ELISA ( Figure 8B ). These data suggest that there is a coordinated and distinct kinetic of infection of DENV-2 in different cell populations in blood and there is a lack of type I IFN production in those cells after infection with this virus, at least at this early time point. The early time point of 12 hpi was chosen to obtain sufficient numbers of pDCs, since these cells have short half-lives and downregulate their specific cell surface markers in a rapid fashion. Nevertheless, we have previously reported that as early as 8 hpi, pDCs are able to produce type I IFN after infection with other viruses, such as NDV [24] .