We observed that relative to isotype control staining, an increased percentage of alloactivated CFSE lo CD4 Ceacam1 2/2 T cells were positive for the alloactivation marker CD25, and that a greater percentage of these cells downregulated CD62L than WT T cells ( Figure 6B-C) , suggesting that more of them became activated. Additionally, an increased percentage of donor Ceacam1 2/2 CD4 T cells had divided to a CFSE lo alloactivated state ( Figure 6D) , suggesting enhanced proliferation in the absence of Ceacam1.