The study was conducted in Lagos State, South Western Nigeria, in June 2011. A quassi experimental, before and after study was done. Participants were DSNOs and assistant DSNOs of the 20 Local Governments in Lagos State. Training materials were received from the Lagos State Ministry of Health,World Health Organisation (WHO) and the Central Public Health Laboratory, Lagos. Pre-and posttests were conducted using questions developed for the purpose. Participants scores were categorised as fail (B50%) and pass (50% and above). The impact of the training was assessed by statistical evaluation of the pre-and posttests results.