Goel and Rai [120] reported that the field isolates of FMDV could be passaged in BHK21 clone 13 monolayer cell culture, which showed characteristic CPE and were readily adapted between 3rd and 5th passage. The CPE usually develops within 48 hours, if no CPE is detected the cells should be frozen and thawed, used to inoculate fresh cultures and examined for CPE for another 48 hours. An alternative to the virus isolation is cell suspension plaque test which also quantify the virus present in sample [121] . However, the cell culture system is laborious, time consuming, and relatively low sensitive. It also requires careful handling of specimens and a biosafety laboratory.