Traditionally, NeuNAc is prepared through extraction from natural sources, such as bird nests, milk, or eggs [5] , through the hydrolysis of colominic acid (a homopolymer of NeuNAc) in a culture broth of Escherichia coli K1 [6] , or through chemical synthesis [7] . Methods for NeuNAc production have included a chemo-enzymatic process [8, 9] , a two-enzyme reaction process [10, 11] , a biotransformation process using E. coli [12] , and an E. coli whole-cell system [13] . However, the requirement for ATP or an excess of pyruvate and the subsequent expensive downstream processing has kept the costs of NeuNAc production considerably high (current market price is $100/g).