Twenty-five of the 142 (17.6%) adult samples that were positive for IgG antibody against HBoV1, 2, and 3, and 17 (12%) that were positive for HBoV4 in the ELISA were negative for IgG against all 4 HBoVs in the cELISA. Among positive serum samples against single HBoV species, there were 26 for HBoV1, eight for HBoV2, and two for HBoV3 based on the cELISA results.