Human and mouse DCs were obtained as described above, and at day 5 of culture, samples of 1610 6 cells were resuspended in 100 ml of DC-medium and were infected for 45 min at 37uC with the indicated MOI of virus (diluted in DC media) or with DC medium (mock group) in a total volume of 200 ml. After the adsorption period, DC medium supplemented with 10% FBS was added up to a final volume of 1 ml, and cells were incubated for the appropriate time at 37uC. siRNA transfection and Dengue infection of human MDDCs 2.5610 4 MDDCs were seeded per well in 96 well plates and transfected with the corresponding siRNA using the StemFect RNA transfection kit (Stemgent), according to the manufacturer instructions. Chemically synthesized 27mer siRNA duplexes were obtained from OriGene Technologies, Inc. The sequences of the STING siRNA oligonucleotides used in this study are as follows: