Lots of work has been carried out to develop and validate diagnostic tests in regard to this disease. Conventional techniques such as complement fixation test (CFT) [23] , serum neutralization tests (SNT) [24] and enzyme-linked immunosorbent assay (ELISA) [25] are still in use for the routine detection of FMDV in clinical samples. Sandwich ELISA is being carried out for the detection of specific FMDV antigens in epithelial tissue suspensions which is usually accompanied by concurrent cell culture isolation and the application of ELISA to any samples showing a cytopathogenic effect [26] . Virus isolation in primary cultures is laborious, expensive, and requires days/weeks (cell passages) before the results are obtained [27] . However, with the introduction of molecular techniques in the field of diagnosis, several techniques based on viral genome detection such as hybridization using DNA probes [28] and the advent of Polymerase Chain Reaction (PCR) technique in the recent past have led to development of several reverse transcription PCR (RT-PCR) procedures for specific detection of FMDV RNA [29] [30] [31] [32] [33] [34] . Because of the reported sensitivity and specificity, RT-PCR has been evaluated as a diagnostic tool for FMDV detection in parallel with ELISA and virus isolation [35] . Another form of PCR, multiplex PCR (mPCR), has also been evaluated for differentiating FMDV serotypes [27, 36, 37] as well as for differential diagnosis with other vesicular diseases such as Vesicular' Stomatitis, Swine Vesicular Disease [38] . The most recent development in the field of diagnosis by nucleic acid detection is the use of thermal cyclers capable of measuring fluorogenic PCR amplification in real-time have become available, making precise quantitation of nucleic acids possible over a wide concentration range. The fluorogenic RT-PCR provides relatively fast result, enables a quantitative assessment to be made of virus amount, and can handle more samples and/or replicates of samples in a single assay than the conventional RT-PCR procedure [39] .Therefore it is seen as a valuable tool to complement the routine diagnosis procedure for FMD virus diagnosis.