Confocal Microscopy of Candidate Proteins Identified by 2D Gel Analysis-As with the SILAC data, we examined using immunofluorescence a number of candidate proteins identified by 2D gel analysis that were altered in abundance following infection. Fig. 5 shows that proteins RBM4 (Fig. 5A ) and eIF6 (Fig. 5B) were both nucleolar antigens in uninfected cells. On the other hand, RBM4 was apparently recruited from the nucleolus into the nucleoplasm but with exclusion from the DBP-rich centers. However, eIF6 became more diffuse with reduced nucleolar location but, notably, without specific exclusion from DBP-rich centers.