To detect the expression level of hOCT1, GUS, BCR/ABL, and ABL mRNA from selected fractions at different time points of IMA therapy, the TaqMan based real-time reverse transcription PCR (RQ-RT-PCR) with absolute quantification was carried out on an ABI PRISM 7300 (Applied Biosystems Inc, Foster City, USA). The RQ-RT-PCR using the Absolute Q PCR Mix (ABgene, Epsom, UK) was performed at 958C for 15 min followed by 50 cycles of denaturation at 958C for 15 s and extension at 608C for 1 min.