We found that the Seeplex Meningitis ACE Detection kit offered high sensitivity and specificity for identifying bacterial and viral pathogens in patients diagnosed with acute meningitis. The LoDs for the 12 pathogens ranged from 10-50 copies/µL, and no cross-reaction with human genomic DNA or reference (clinically identified) bacterial and viral pathogens was evident, indicating the high specificity of the kit.