Objective: Fungal organisms are commonly seen on smears. Some unusual species as Aspergillus and Penicillium are so rare and generally seen via contamination. In this study, we investigated the origin of the many extraordinary fungal organisms seen on Pap smears in series in only a few months. Method: In Pathology department of Mardin Maternity Hospital, we observed both smear and vaginal discharge materials, came from the same hospital but different clinicians, for 3 months. Results: 149 smears came from clinician A and 335 smears from clinician B. Curious fungal organisms with huge branching hyphas and macrochonidias were seen in 15 smears among 149 smears (2 Aspergillus and 4 Penicillium species were recognized morphologically). No growth was observed in cultures. Interestingly, among all 335 smears came from clinician B, there was no abnormal funguses. The smear samples with unusual fungal components all came from the same gynecologist. This made us strongly consider the probability of contamination. Conclusion: Penicillium and Aspergillus spp. are extremely rare in vaginal smears. In our study, these funguses are thought to be airborne passed from the thick layer of the mold spreading on the ceiling of the office. Objective: Nearly all cervical cancers are related to human papillomavirus (HPV) infection. Hybrid Capture 2 -HC2 (Qiagen, Hilden, Germany) is the method to determine the presence of high risk HPV (HR HPV) in cytology samples with the best clinical sensitivity. Method: In our study we compared the results of the HPV test in 742 ThinPrep® cervical samples using first HC2 and secondly Cervista (Hologic, Madison). Samples were divided in two groups for the statistical analysis. The first group were HSIL cases confirmed by biopsy (n =65) and the second group cytology samples reported as negative, ASC-US or LSIL (without biopsy or biopsy, not HSIL) (n=677). Results: Overall, concordance between the two techniques was 92 % (683/742). There were 23 cases HC2+ Cervista−, and 36 cases HC2 −Cervista+. The average HC2 viral load in discordant cases was 2.83. In our series the HC2 sensitivity for ≥ CIN2 was 100 % (65/65) and the specificity was 85.4 % (578/677). Cervista results were 98.5 % (64/65) and 83.3 % (564/677), respectively. In the HSIL cases the coincidence was 98.5 % (64/65). Conclusion: Cervista results showed good clinical sensitivity and high concordance with HC2, with a slightly lower specificity. In conclusion, according to our data the determination of HPV with Cervista is comparable with HC2.