Effi ciently transfect lung enzyme [49] , angiopoietin-1 [51] , HSP-70 [52] , epithelium [14, 16] apolipoprotein A-1 [53] , and Na + ,K + -ATPase pump Can deliver larger genes [55] genes attenuate experimental ALI Well tolerated in lower doses [1, 3] Adenoviral delivery of IL-10 gene attenuates zymosan ALI at low doses, but is harmful at high doses [58] Adeno-associated virus Good safety profi le; less Limited transgene size AAV vector gene transfer demonstrated in multiple vectors (ssDNA genome)