We present DetectiV, a package for R [10] containing functions for visualization, normalization and significance testing of pathogen detection microarray data. R is a freely available statistical software package available for Windows, Unix/ Linux and MacOS, meaning DetectiV is a platform independent solution. DetectiV uses simple and established methods for visualization, normalization and significance testing. When applied to a publicly available microarray dataset, DetectiV produces the correct result in 55 out of 56 arrays tested, an improvement on previously published methods. When applied to a second dataset, DetectiV produces the correct result in 12 out of 12 arrays.