Based on the assessment results, the criteria for T cell epitope prediction using Surflex-Dock were defined as follows: a) the peptide made close contact with the groove and it docked in the correct direction; b) the anchor residues bound to the anchor site in a rational conformation; and c) the docking score of the complex was greater than 8.0. A total of 25 potential T cell epitope peptides were predicted (eight for B4, six for B12, two for B15, and seven for B19). Nine peptides, which marked in bold in table 1, were selected to test their T cell responses in vitro. The binding energies of those complexes were also calculated. The results are shown in Table 1 . 