We have recently developed Cucumber green mottle mosaic virus (CGMMV) as a candidate for expressing antigenic peptides in plants [7] . CGMMV is a tobamovirus with a genome size of ∼6. 4 kb which has been well characterized both biologically [8, 9] and structurally [10, 11] . In this study, a truncated dengue virus type 2 envelope (E) protein binding region from amino acid 379 to 423 (EB4) was inserted into the end of the coat protein (CP) open reading frame (ORF) of a previously constructed CGMMV fulllength clone, pCGT7X [7] . The antigenic peptide was chosen based on a recent study that suggests its importance in enabling dengue virus to bind to specific host cell receptors (S. Abu Bakar personal communication). The present study explores the possibility of extrapolating the CGMMV antigenic epitope presentation system for developing diagnostics Figure 1 : Position of primers in constructed plasmid clones. Primers 4-8 were used to amplify the EB4 gene from pCANTAB 5E. Plasmid pCGT7X and amplified EB4 fragments were digested with HindIII and ligated together to obtain the respective plasmid clones as shown. HindIII is the insertion site of EB4 at the end of CGMMV CP. PCR amplification using primer pairs 1-9, 3-9, and 2-7 will yield amplified products of approximately 6.5 kb, 0.85 kb, and 2.2 kb, respectively. and potentially therapeutics against dengue. The study was also used to challenge the size limits of foreign gene insertion into the CGMMV vector as in the previous study the hepatitis B surface antigen (HBsAg) used was only 33 amino acids [7] .