ORMA, then, retrieves the sequences flanking each of the putative discriminating positions. Actual oligonucleotide design is dependent on the molecular application chosen. The maximum length and the thermodynamic model for calculation of the parameters of the probes can be specified by the user. For the LDR experiments here described, two oligonucleotide probes are designed, one upstream (Discriminating Probe, DS, comprising the discriminating position) and one downstream (Common Probe, CP) of each position.