Since the hydrophobicity of the linker appeared to influence splicing correction efficiency, we have compared a series of PMO conjugates (compounds 8-11 in Figure 2A ) with the same spacing (a 6-carbon atom spacer as in (R-Ahx-R) 4 -PMO) but with varying sidechain hydrophobicities. Figure 1C . 5, (R-Ahx-R) 4 -PMO; 13, (r-Ahx-R) 4 -PMO. (C) Hydrophobicity chromatography. Samples were treated and data were processed as described in the legend of Figure 2B . (D) Heparin affinity chromatography. Samples were treated and data were processed as described in the legend of Figure 1B . ÃÃÃ and ÃÃ Indicate statistically significant differences; NS indicates that the difference is not statistically significant.