When it comes to pseudoknots, many structures have been published based on a secondary structure predicted by free energy minimization. These predicted secondary structures are used as a working model and refined using experimental techniques such as chemical and enzymatic probing. However, the native structure remains unsolved unless tertiary structure determination methods such as X-ray crystallography are used. Testing structures that are based on computer predictions with no experimental support creates a bias in the benchmark and will be avoided in this evaluation.