Hepatitis B virus (HBV) genotypes show distinct geographical distributions and virological and clinical differences. In some of genotypes, specific substitutions and mutations have been described in association with hepatitis B e (HBe) protein expression and viral replication. In this study, genetic characteristics of HBV genotype E (HBV/E) were investigated using clinical samples obtained from 12 Hepatitis B e antigen (HBeAg)-positive, and 11 anti-HBe-positive asymptomatic carriers (ASCs) in West-Africa. Full-genome analysis of isolated HBV strains revealed strong association between precore (PC) mutation and HBeAg to anti-HBe seroconversion.