PCV belongs to genus of porcine circovirus, family of porcine circovirus. It has two genotype, PCV genotype I and PCV genotype II, and it is the smallest virus which has been discovered so far [14] . Among the different genotype, PCV genotype II infection and its related diseases have become one big problem across the globe for pig feeding, which threatens greatly to normal development of the industry of pig feeding. The PCV genome is a single-stranded negative circular DNA, and very small; full length of the PCV genotype I is only 1,759 bp, and PCV genotype II, 1,767 bp or 1,768 bp. The genome contains 11 open reading frames (ORF), among which, ORF1 encodes replication-associated proteins (Rep and Rep'); ORF2, structural proteins (viral capsid proteins, Cap); ORF3, toxicity-associated proteins, which can cause apoptosis [15, 16] . By analyzing the whole sequence of PCV genome, it is found that ORF2 has smaller selective pressure than ORF1, and more mutation. Nucleotide sequences among various strains in the same genotype are very conservative, their homology is over 90%, while similarity between nucleotide sequences from various strains respectively from the two genotypes is less than 80% [17, 18] . However, so far, studies have not related to usage of PCV codons. Explanation of codon usage pattern of PCV has significance on PCV evolution, gene prediction, gene classification, design of high expressed genes and viral vectors, and understanding of interaction between PCV and its host cells. Therefore, in this study, we first performed comprehensive analysis on codon usage pattern of PCV genome and the related factors affecting on codon usage. This study will play a major role in explanation of evolution process of PCV genome and further studies.