We also screened the same yeast library against serum samples from goats immunized by the 2009 A(H1N1) influenza virus to examine whether different animal models would yield a similar profile of antigenic peptides. Following sorting and sequencing, 78/100 clones were confirmed as in-frame, among which 55 tested positive by the goat anitsera in FCM (RAYS ratio $2). Surprisingly, alignment of the positive antigenic peptides with the full HA protein shows a distribution strikingly different from the alignment obtained using the mouse antisera in several aspects. First, 78% of the antigenic peptides are located in the HA1 region and have shorter lengths (54632 aa), while only 9% are in the HA2 region (96619 aa). Second, 13% of the peptides are in the region across HA1 and HA2 (66631 aa) (Fig. 2, 