To measure the level of intracellular lipids following 72-hour recombinant adenovirus expression or 48-hour PF-429242 treatment, cellular extracts were harvested in 1% triton-X 100 in PBS (for phospholipid, cholesterol, and protein assay) or 5% triton-X 100 in H 2 O (for triglyceride assay). To extract triglycerides, samples were slowly heated to 90uC and brought to room temperature, twice. Total cholesterol, cholesterol esters (Amplex Red Cholesterol Assay kit, Invitrogen), phospholipids, and triglycerides (EnzChrom, BioAssay Systems, Hayward, CA, USA) were quantified using commercially available kits. Lipid levels were normalized to cellular protein content (DC Protein Assay, Bio-Rad, Hercules, CA, USA).