In contrast to the weak or non-neutralizing hMAbs isolated from donor 033, several hMAbs from donor 013 displayed strong typespecific DENV2 neutralization. Of note, even hMAbs from donor 013 that bound to more than one serotype (dengue subcomplex or complex MAbs) displayed type-specific neutralization of DENV2, which was the serotype that infected donor 013. Thus, by biasing the selection to enrich for rare rE protein binding antibodies, we generated hMAbs from donor 013 that were functionally similar to the polyclonal immune serum from the same donor that neutralized only DENV2. It is unclear how the results with hMAbs relate to the neutralization properties of polyclonal immune serum from these donors. One possibility is that the abundance of hMABs reflects the functional properties of antibodies in immune sera, where a small fraction of DENV-specific antibodies in immune sera are responsible for neutralization. Alternatively, it is conceivable that individual antibodies that are weakly neutralizing become strongly neutralizing due to cooperative effects that only occur in a polyclonal milieu. Further studies are needed to understand how the properties of hMABs from DENV immune donors relate to the properties of circulating serum antibody.