Subtypes of SAa2-3Gal glycans with different subterminal sugars present varied affinity to MAA/MAL-I and MAL-II. Whether the SAa2-3Gal glycan is not expressed on goblet cells was further evaluated by MAL-II (Vector Laboratories). Surprisingly, 0.3760.12 mucin + cells (n = 594) were double-stained with MAL-II (Fig. 1F ), indicating that a glycan subtype that shows a high affinity for MAL-II only, was expressed on the goblet cells. We also found that only 0.3360.09 b-tubulin + cells (n = 230) can be labeled with MAL-II (Fig. 1C ). In addition, about half basal cells were MAL-II positive (0.5360.12, n = 265) (Fig. 1H ).