Detection of the signs of HIV epidemic transition from concentrated to generalized stage is an important issue for many countries including Ukraine. Objective and timely detection of the generalization of HIV epidemic is a significant factor for the development and implementation of appropriate preventive programs. As an additional method for estimating HIV epidemic stage, the spatial analysis of the reported new HIV cases among injection drug users (IDU) and other populations (due to sexual way of transmission) has been recommended. For studying new HIV cases in small societies, relative risk (RR) rates are preferred over incidence indicators. Spatial clustering based on the calculation of RR rates allows us to locate the high-risk areas of HIV infection with greater accuracy.