Sephadex G-25 (GE Healthcare), BioGel P-2 fine (Biorad), Sephadex G-10 (GE Heathcare), HiTrap Q HP (GE Healthcare) and HiTrap SP HP (GE Healthcare) matrixes were tested for the fractionation of the different solutions of Hypeps. Fractionation was monitored by measuring the absorbance of the collected fractions at 214 nm. Collected fractions were desalted, then frozen at -70°C and lyophilized. After lyophilization the fractions were resuspended in M199 so they would be compatible with cell culture.