We have previously generated highly active, recombinant human MHC-I (HLA-I) molecules and accompanying highthroughput assays and bioinformatics prediction resources. Here, we transfer the underlying approaches to an important domesticated livestock animal, the pig, and its MHC system, the SLAs. MHC-I molecules are composed of a unique and highly variable distal peptide-binding platform consisting of the alpha 1 (α 1 ) and alpha 2 (α 2 ) domains of the MHC-I heavy chain (HC) and a much more conserved proximal immunoglobulin-like membrane attaching stalk consisting of the alpha 3 (α 3 ) domain of the HC noncovalently associated with the soluble MHC-I light chain (β 2 m).