Two overlapping potential nuclear localisation signals' (NLS) were identified in the aa sequence of PHSV NS4 ( . Although all of these NLS were monopartite, the GIV NS4 was found to contain a bipartite NLS (position 113-141: RKRGLEFLLLPLHEYVTHCAKEDIR-IYES). The prediction cut-off scores for all these NLS as defined by PredictNSL and cNLS ranged from 4 to 8, indicating dual nuclear/cytoplasmic localisations of a given protein [38] .