The parvovirus sequences discovered in the investigated bushpigs showed closest similarity to PPV4. Porcine parvovirus 4 was originally discovered in 2010 in samples collected from pigs in North Carolina in 2005 after an outbreak of acute-onset of disease with high mortality [12] . Subsequently, PPV4 was reported in pigs in China where 1.84% of the investigated pigs were PPV4 positive [13] . The genome of parvoviruses consists of two major open reading frames (ORF) encoding the non-structural and the capsid proteins. However, the genomes of PPV4 as well as of PBoV contain an additional third ORF [12] . The parvovirus sequences obtained from this study could be found in the capsid and in the non-structural ORF. All reads classified as parvovirus gave significant similarity to PPV4 through Blastx searches. A PCR with primers designed to amplify PPV4-like sequences from the original extracted genetic material showed the presence of this virus in one of the three bushpig sera. Sequencing of the PCR product (GenBank accession number: JQ277337) confirmed correct amplification and sequence analysis showed that at protein level (84 amino acids), the product displayed a 75.9-77.1% sequence similarity to the PPV4 sequences available in GenBank. In addition, the phylogenetic tree generated from these data ( Figure 1 ) grouped the sequence with PPV4 when analysed together with sequences from all the different parvovirus genera (Parvovirus, Erythrovirus, Dependovirus, Amdovirus and Bocavirus) and PPV4. However, the tree also confirms the divergence of the bushpig PPV4 from the other PPV4. PPV4 has previously been reported in domestic pigs only in USA and China [12, 13] . However with this study we show the presence of a PPV4 variant in a wild suid in Uganda. Figure 1 Neighbour-joining phylogenetic tree of Parvovirus. The Neighbour-joining tree shows the phylogenetic relationship between the bushpig PPV4 sequence (84 amino acid long) and 49 sequences available from GenBank. The bushpig PPV4 is indicated with ♦.