Liver homogenates and serum were tested for cytokines by standard sandwich ELISA. IFN-c, IL-12p70 and TNF-a were assayed by DuoSet (R&D Systems). IFN-a was measured by VeriKine mouse ELISA kit (R&D Systems). Limits of detection were 15 pg/mL for DuoSets and 12.5 pg/mL for VeriKine ELISA kits.