Previous studies have shown that capsid modifications, as well as some transgene incorporations, can compromise viral growth characteristics. In order to ensure that normal viral growth was not inhibited by epitope and/or transgene incorporation in the viral particle/genome we performed growth kinetic assays on vectors used in this study. To obtain a quantitative understanding of this effect all respective vectors were titered in HEK293 cells until full CPE was achieved in order to determine growth kinetics. The data illustrates that the unmodified Ad5 had the highest titer (,1610 14 IFU/ml) and produced full CPE at 96 hours postinfection. AdCMVGag also produced full CPE at 96-hours postinfection, however, AdCMVGag had a titer, several orders of magnitude lower than that of the Ad5. Ad5/HVR2-MPER-L15(Gag) showed comparable titers to that of AdCMVGag. The control vector expressing full-length envelope protein (AdCMVEnv) reached full CPE after 120 hours post-infection. Ad5/HVR2-MPER-L15DE1 yielded the slowest growth kinetics and relative titer, reaching full CPE at 192 hours post-infection (Figure 4) . These growth kinetic patterns were similar to other capsid and transgene modified vectors developed in our laboratory [31, 35] .