Background : To detect circulating CD4 + CD25 + regulatory T cells and toll-like receptor(TLR)2 and TLR4 expression on the peripheral blood mononuclear cells (PBMCs) of patients with HBV-related liver cirrhosis (LC), and to explore the correlation between them. Methods: PBMCs isolated from 14 LC patients , 21 chronic hepatitis B (CHB) patients and 16 normal controls(NC) were stained with fluorescent labeling anti-TLR2-PE, anti--TLR4-APC, anti--CD14-FITC monoclonal antibodies and anti-CD4-PerCP anti-CD25-FITC anti-CD127-PE. Samples were collected and detected of three-color immunofluo rescence by flow cytometry. Results: The expression of TLR2 and TLR4 were significantly up-regulated in patients with LC than those in the controls.The expression of TLR2 was significantly increased in patients with LC than those in patients with CHB, but there were no differences of TLR4 expression between LC and CHB.Treg/CD4 + T cells were significantly increased in patients with CHB than those in patients with NC and LC, but there were no differences between LC and NC. There were no correlation between the expression of TLR2,TLR4 and Treg in patients with LC . The expression of TLR2 and TLR4 on PBMCs in patients with LC were positive correlation.The expression TLR4 and HBV DNA level were negative correlation in patients with LC. Conclusion: The expression of TLR2 and TLR4 were up-regulated on PBMCs in patients with LC. It seems to be expression of TLR2 and TLR4 invovlved in the pathogenesis of LC.