The VSA subset VSA PAM is expressed by P. falciparum involved in pregnancy-associated malaria (PAM). VSA PAMspecific IgG mediates acquired immunological protection against PAM, and the PfEMP1 variant VAR2CSA appears to be the main or only target of these antibodies. VAR2CSA is therefore the leading candidate for development of vaccines against PAM. We used monoclonal human IgG antibodies to select erythrocytes infected by two genotypically distinct laboratory P. falciparum clones derived from nonpregnant donors for expression of VSA PAM . Parasites acquiring expression of VSA PAM following selection showed increased levels of transcripts encoding the PfEMP1 variant VAR2CSA, which appears to be the only PAM-type VSA in the P. falciparum genome. The results obtained in the study are important for several reasons.