In the present study, a negative-pressure isolation chamber was designed and an initial attempt at building the chamber was completed. Two extraction blowers are used, one working and one on standby, to ensure the isolation chamber internal pressure is both stable and secure. The isolation chambers contain two DPTE systems for the transfer of experimental materials and the collection of excrement into a small container. The sterilization chain for the isolation chamber is composed of a peracetic acid sterilizer and its associated pipeline. A programmable logic controller (PLC) system and WINCC 6.0 system are used to automatically control and monitor the isolation chamber. Rapid pressure changes and the exhaust system are adjusted via a variable frequency drive inlet air blower and extraction blower. Isolation chamber parameters are menu-managed by the automatic control system allowing the researcher to set different isolation environmental parameters depending on experimental requirements.