The transcriptome map helped to identify incorrect annotation of start codons in the genome. Transcriptional mapping does not provide direct evidence of translational start sites. However, location of identified transcriptional start sites suggest that the annotated start codons are incorrect, an observation that is confirmed by BLAST comparisons against homologous genes in other bacterial species. Transcriptional mapping revealed genes where the 59 untranslated sequence extended well beyond the translational start. BLAST comparisons indicated that these genes have either nonsense or missense base changes relative to homologous genes in other bacterial species, causing apparent ''truncated'' proteins compared with those in other species. Further work is needed to determine whether these 59 untranslated regions serve regulatory functions or they are vestigial.