Objective: Desmoids is a locally recurrent and invasive but not metastatic tumor. Our aim was to study immunohistochemical profile of primary and recurrent desmoids. Method: Complex morphological study of 16 abdominal desmoids. All the tumors were divided into 3 groups: primary, first recurrent and second and more recurrent cases. We used antibodies against β-cathenin, COX-2, APC, survivin and Ki-67 for immunohistochemical study. Results: Nuclear and cytoplasmic β-cathenin expression levels were significantly higher in recurrent than in primary desmoids. APC cytoplasmic expression level was also significantly higher in recurrent tumors. We revealed only cytoplasmic COX-2 expression in desmoids, and its level was significantly higher in recurrent tumors with the activity increasing accordingly to the number of recurrences. Survivin expressed both in nuclei and cytoplasm of the tumor cells and its expression levels were significantly lower in recurrent desmoids. Conclusion: Revealed immunohistochemical properties of primary and recurrent desmoids reflect tumor transformation and progression and could be used as additional prognostic markers in this disease. Objective: Many bone and soft tissue sarcomas (BSTS) are aggressive tumors with fatal prognosis. The importance of angiogenesis for the growth and progression of solid BSTS is now well recognized. A variety of chemokines have been described that either promote (angiogenic) or inhibit (angiostatic) angiogenesis. The aim of this study is to characterize the expression profile of some chemokines in a series of xenotransplanted human BSTS. Method: One Ewing sarcoma (ES), 1 grade 3 chondrosarcoma (Chs), 1 osteosarcoma (Os), 1 synovial sarcoma (SS), 1 fibrosarcoma and 1 gastrointestinal stromal tumor (GIST) were xenotransplanted into the backs of nude mice (athymic Balb-c nude mice). When the tumor size reached the 3 cm, animals were sacrificed and tumors analyzed for the expression of CXCL1/2/3, CXCL9 and CXCL10, using two-color staining fluorescence on each slide under a confocal microscope (Olympus FV1000). Results: We observed that angiostatic chemokines (CXCL9 and CXCL10) presented higher expression than angiogenic (CXCL1/2/3) chemokines in ES, grade 3 Chs, Os and GIST whereas fibrosarcoma and SS were more positive for angiogenic chemokines. Conclusion: The expression profile of angiostatic and angiogenic chemokines depends on the type of BSTS and could be related to their different biological behaviour. Other elements such as angiogenic or pro-inflammatory markers should also be considered. Objective: The wide range of differential diagnostic possibilities of rhabdomyosarcomas (RMS) shows the need for more specific and sensitive markers. Low survival rates of high risk RMS patients require new prospective therapeutic targets. Method: Archival material FFPE samples of 15 RMS (12 embryonal -eRMS, 3 alveolar -aRMS) and non-RMS soft tissue tumors were evaluated by immunohistochemistry for myogenin, MyoD1, EGFR, VEGF, COX-2, p-Akt and p-mTOR expression. The presence of PAX3/7-FKHR forming translocations, myogenin, MyoD1, gamma subunit of fetal acetylcholine receptor (AChR) and K-RAS mutational state were determined by RT-PCR. Results: 10/12 eRMS and 3/3 aRMS showed expression of myogenin, MyoD1 and gamma AChR. Non-RMS tumors were negative. Translocations were only found in aRMS. EGFR expression was characteristic for eRMS, without the presence of activating K-RAS mutation. Strong expression of VEGF was detected in all samples. p-AKT and p-mTOR showed overlapping expressions in 86,7 % RMS. In most of the samples weak COX-2 positivity was demonstrated. Conclusion: Myogenin, MyoD1 and the fetal AChR are specific and sensitive diagnostic markers of RMS. RMS subtypes are identifiable by PAX3/7-FKHR detection and probably EGFR expression. The results indicate VEGF, EGFR, COX-2 and Akt-mTOR pathway directed therapy to be considered in RMS. Supported by ITMS 26240220052.