Expression of CD44 and CD24 in primary ductal invasive carcinoma of breast and metastatic lymph nodes M. Gudadze * , K. Kankava, T. Javakhishvili, M. Sarishvili, G. Burkadze * Central University Clinic, Dept. of Pathological Anatomy, Tbilisi, Georgian, Republic of Georgia Objective: Cancer stem cells are believed to have certain role in progression, metastatic potential and outcome of ductal invasive carcinoma of breast. Method: The archived materials of 2008-2012 years from the Department of Pathological Anatomy, N. Kipshidze Central University Clinic were used for the research. 196 cases of ductal invasive carcinoma were chosen for the study. The CD44, CD24 immunohistochemistry (Biocare Medical) was used for staining. Evaluation was performed using SPSS software. Results: The results showed: 68.5 % of grade II cases and 90.5 % of grade III cases were lymph node positive. CD24 positivity in grade II tumors was seen in 29.6 % of primary site and 45.9 % of metastasis; in grade III tumors-50 % of primary site and 55.2 % of metastasis. CD44 positive cases in grade II-48.1 % for primary tumor and 27 % in metastasis, for grade III-73.8 % for primary tumor and 39.4 % for metastasis.