rhMBL Binds Both pdmH1N1 and H9N2/GI Viruses A microtiter capture assay demonstrated that MBL could bind to pdmH1N1 and H9N2/G1 in vitro ( Figure 1 ). The MBL-virus binding occurred in a dose-dependent manner ( Figure 1A and 1D). Similarly, increased amount of virus could also result in increased binding by MBL ( Figure 1B and 1E) . MBL utilizes the CRD to recognize pathogens in a calcium-dependent manner [30] . Further addition of ethylenediaminetetraacetic acid (EDTA) in the assay inhibited the binding of MBL to both strains of influenza virus ( Figure 1C and 1F) , suggesting that the binding occurred through the CRD of MBL.