The various quaternary associations in the JRL family exhibited different contacts between protomers. A previous report indicated that the buried interface of the Calsepa dimer is 1,327 Å 2 by a probe with 1.6 Å radius [27] . Here, we analyzed the buried interface of the selected structures from the above comparison by using the PDBe PISA service with 1.4 Å radius [34] . The buried interface area from tetrameric IPO encompasses 1,539 Å 2 , which is larger than that of Calsepa (1,202 Å 2 ), PPL (1,294 Å 2 ), Banlec (750 Å 2 ), Heltuba (736 Å 2 ), and Jacalin (1023 Å 2 ). The N terminus of the protomer in the JRL family has an important role in the quaternary association by swapping in the interface and then forming a dimer, tetramer, hexamer, and octomer. To compare the difference between the tetrameric Jacalin ( Figure  S1A ) and the tetrameric IPO ( Figure S1C ), the tetramer of Jacalin showed a looser interface than that of IPO. Therefore, IPO formed a different compact tetramer.