Single cell suspensions from bone marrow or spleen from transgenic or wild-type mice were blocked by anti-mouse CD16/ 32 antibody (Clone: 93, Biolegend, San Diego, CA 92121), and (4) (5) (6) in liver (1, 4) , kidney (2, 5) , and small intestine (3, 6) . B. RT-PCR analysis for Ceacam1 isoforms in transgenic (7) (8) (9) or wild type mice (10) (11) (12) in liver (7,10), kidney (8, 11) , and small intestine (9, 12 Single cell suspensions (1610 6 /mL) from spleen from transgenic or wild-type mice were cultured in a 24-well plate for 72 hours in the absence or presence of 1 mg/ml plate-bound anti-CD3 mAb (145-2C11) and 2 mg/ml soluble anti-CD28 (37.51; BD Pharmingen). Cells were harvested, stained and analyzed as above methods by FACS.