No axonal loss was identified at 5 to 7 days pi in optic nerves of RSA59-or RSMHV2-infected mice (Figs. 4AYC). At day 30 pi, RSMHV2-infected mice continued to show no axonal loss (Fig. 4D) , whereas optic nerves from RSA59infected mice showed regions of mildly reduced axonal staining, with focal areas of axon loss intermixed with areas of normal axons (Figs. 4E, F) . Quantification of the area of axonal staining across all sections of optic nerves of RSA59infected mice demonstrated a small but significant decrease compared with optic nerves of either RSMHV2-infected or mock-infected control mice (p = 0.0306) (Fig. 4G ).