To assess the effect of viral delivery of adult globin to B. marinus tadpoles, seven groups of tadpoles were infected at day 6 (stage 20) post hatching. Group A, uninfected cell culture supernatant; Groups B, C and D -10 2 , 10 3 and 10 4 TCID 50 /mL of the negative control virus, rBIV/neo r respectively; Groups E, F and G -10 2 , 10 3 and 10 4 TCID 50 /mL of the test virus, rBIV/neo r / Adglob respectively. Each group of 116 tadpoles was infected for approximately 6 h in 2 L of water containing the appropriate concentration of virus, rinsed in clean water for 5 min then divided into 4 tubs each containing 29 tadpoles. These tubs were randomly dispersed around 3 different rooms to allow for statistical variation due to the position of the tub.