Cells were analyzed on a multi-color FACSAria flow cytometer (BD Biosciences). For Blood samples between 500,000 to 1,000,000 total events and for liver biopsy samples between 50,000 to 200,000 total events were collected. Data analysis was performed using FlowJo version 8.6 (Treestar Inc., San Carlos, CA). Polychromatic FlowJo data were analyzed with PESTLE software, and pie-chart graphs were generated using SPICE software (obtained from M. Roederer, National Institutes of Health, Bethesda MD).