In previous section, we established a method for estimating the true concentration of the target molecules in the DNA sample from the count of positive chambers as well as the 95% confidence interval for this estimation. We also showed how the sampling distribution gl l is related to the sampling distribution f(p). In CNV, the goal is to determine ratio of true concentrations of two genes, one being reference gene and the other being test gene, and associated confidence interval, which we now accomplish in next subsections. Table 1 . Comparison of the metrics of histogram, shown in Figure 5 , of number of positive chambers obtained in simulation with those predicted by the theory. 