Osteoblastlike cells (MG63) were grown in sterile Falcon wells (Becton & Dickinson, Franklin Lakes, NJ) containing Eagle's minimum essential medium supplemented with 10% fetal calf serum (Sigma-Aldrich, St Louis, MO) and antibiotics (penicillin 100 U/mL and streptomycin 100 lg/mL; Sigma-Aldrich). Cultures were maintained in a 5% CO 2 humidified atmosphere at 37°C. For the assay, cells were collected and seeded at a density of 1 9 10 5 cells/mL in two multiwells (one for the control and one for the treated). Each multiwell was comprised of six wells, 9-cm 2 , in which 3-mL of complete medium was added.