Epitope mapping of EDIII binding hMAbs DENV antibody escape mutant viruses were selected for by infecting Vero cells with DENV2 (strain S-16803) in the presence of hMAb concentrations estimated to neutralize greater than 99% of infectious virus (i.e. 1.0 mg/ml for DVC 3.7 and 1.5 mg/ml for DVC 10.16). Equivalent GC copy numbers of control and antibody treated viruses were repeatedly passaged in the presence of hMAbs until equivalent DENV genomic copy numbers were observed for MAb treated and control samples (4-6 passages under antibody pressure). Escape mutant viruses were plaque purified and amplified. E genes were amplified by RT-PCR and sequenced to identify mutations linked to antibody escape. Anti-body binding sites were also mapped by using yeast cells expressing a library of EDIII as previously described [33] . Mutations were mapped onto the DENV2 EDIII structure using the atomic coordinates of DENV2 EDIII (RCSB accession number 1OAN) and displayed using PyMOL Molecular Graphics System, Version 1.3 (Schrödinger, LLC).