Another source of controversy in lipid raft research which still has to be resolved is determined by the lack of suitable detection techniques in living cells. This resulted in estimation of the putative size of lipid rafts in the range of 5-700 nm. In the last decade a number of new techniques assisting in estimation of raft size and helping in their visualization has been developed and applied (for review see Simons et al., 2010) . They include single molecule spectroscopy and super-resolution microscopy such as fluorescence recovery after photobleaching (FRAP), stimulated emission depletion (STED), Förster resonance energy transfer (FRET), total internal reflection fluorescence (TIRF), and fluorescence correlation spectroscopy (FCS) techniques. However, even using these techniques the subwavelength lipid domains have never been directly visualized but their size was predicted to be <20 nm (Eggeling et al., 2009) . Despite the limitations these new techniques confirmed the existence of nanoscale cholesterol-based assemblies of lipids and proteins in the membranes of living cells and allowed to characterize further their dynamics and properties.