The L11 P-site loop mutants confer temperature-and drug-specific growth phenotypes displayed roughly wild-type growth. Cold sensitivity was assessed at 20 C and both mutants grew at wild-type rates. 51-4A showed enhanced growth at 37 C relative to itself at 30 C, while mutant 54-7A was similar to wild type. R51A grew at wild-type rates at 30 C and 37 C but showed enhanced growth at 20 C. The Y52* mutants displayed mutant-specific effects on growth rates at 30 C, but did not confer significant phenotypes at either 20 C or 37 C. F57A had wild-type growth rates at all temperatures, while R61A showed depressed growth at 30 C, which was rescued at 37 C.