Objective: In last years appear more cases with multiple basal cell carcinomas (BCC). Skin immune system reaction is especially important in nonsurgically treated cases. Aim of study is to evaluate the dynamics of CD 1a, CD 68, CD8 marked cells in BCC and in skin adjacent to tumour (5 and 10 mm) before and after the treatment with cryotherapy and imiquimodum. Method: From 68 BCC cases with multiple tumours investigated immunohistochemically, in dynamics we have characterized 8 patients. Antibodies for CD1a, CD8, CD68+ cells in derma and epidermis were used. They were evaluated in 3 fields of vision (400×) before and after treatment. 5 % imiquimod cream and double freezing was used. Control group: three normal skin samples. Statistical analyses of results were done. Results: After treatment were erythema, then crust and exfoliation. Microscopically expressed fibrosis instead BCC was found. Amount of immune cells before and after treatment were: CD8 25±3,1/34±4,8 (also in epidermis), Langerhans cells 7±2,3/14±3,4, CD 68 19±3,8/10±2,9. Conclusion: All immune cells of skin react to the combined treatment of BCC in radius of 5 mm. There is significant statistical difference between the numbers of Langerhans cells in normal epidermis and in BCC cases treated with cryomethod and imiquimodum.