The selected a-helical coiled coil containing proteins were further tested on their possible surface location and GPI anchoring by using the following programs: identification of potential signal peptides, SecretomeP and SignalP (http://www. cbs.dtu.dk/services/) [26] ; transmembrane spanning regions (TMPRED http://www.ch.embnet.org/software/TMPRED_ form.html and TMHMM http://www.cbs.dtu.dk/services/ TMHMM; [27, 28] ), and GPI-anchored proteins (http://mendel. imp.univie.ac.at/sat/gpi/gpi_server.html; [29] ) and prediction of sub-cellular localization (pTARGET http://bioinformatics. albany.edu/,ptarget; [30] ). To identify the PEXEL-like motifs in sequences of the selected proteins we used the following pattern [ 