Western blotting was done with anti-renalase monoclonal antibodies as the primary antibodies. The results showed that mesangial cells (Fig. 6A) , podocytes (Fig. 6C ) and renal tubular epithelial cells (Fig. 6E ) cultured in vitro also could express renalase. But only tubular epithelial cells' supernatant could be detected the expression of renalase, as shown in Fig. 6F .