Resequencing technology allows a sequence to be determined at less than one tenth the cost of traditional Sanger sequencing, but it is less accurate with some types of sequences and requires a closely related reference sequence. Other methods of resequencing [9, 12] are likely to differ from CGS in cost and accuracy. The control experiment presented here comparing E. coli strains W3110 and MG1655 can now be performed with these other technologies to allow cross-comparison.