The nearly full-length genomes of 2 strains of bovine partetravirus (5096-5240 nucleotides) and 4 strains of ovine partetravirus (2 from liver samples and 2 from spleen samples) (5249 nucleotides) were determined. Similar to our earlier attempt at sequencing the complete genomes of other PARV4-like animal parvoviruses [19] , the obtained genome lengths were shorter than expected, as sequencing of the ends of the genomes was hampered by the presence of extensive hairpin structures. Pairwise comparison of the genome sequences of the parvoviruses was performed. Among the presently identified bovine partetraviruses, HK4 was highly similar (99.3299.4% nucleotide sequence identity) to the bovine partetraviruses reported previously, while HK5 showed much greater sequence divergence (90.0-90.1% nucleotide sequence identity). The genome sequences of the ovine parvoviruses were 100% identical, and they exhibited 68.2-68.3% nucleotide sequence identities to that of the bovine partetraviruses and around 64.0% nucleotide sequence identities to that of the porcine partetraviruses. Results of further sequence comparison with related parvoviruses were shown in Tables 1 and 2. All sequenced virus strains possessed a genome organization typical of parvoviruses. There were two large non-overlapping ORFs, with ORF1 encoding a non-structural polyprotein NS1 and ORF2 encoding overlapping VP1/VP2 capsid proteins and a small conserved putative protein. A small non-coding gap of around 115 nucleotides was found between the two ORFs. Inverted terminal repeats were found at the 59 and 39 ends of the viral genome. Further sequence analysis was not performed for the bovine partetravirus HK4 due to its high genetic similarity with previously characterized bovine partetraviruses. For bovine partetravirus HK5, the predicted NS1 protein consists of 652 aa and is 73.9 kDa in molecular weight. Conserved sequence features including helicase and ATPase domains are present, in agreement with the non-structural functional role of NS1 in parvovirus replication. The VP1 protein is predicted to contain 931 aa and is 102.8 kDa in molecular weight, which is comparable to that of previously identified bovine partetraviruses and larger than the Similar to other partetraviruses, there exists a putative third ORF in a different reading frame within the VP1u region for both bovine partetravirus HK5 and ovine parvovirus. In agreement with previous studies, the phospholipase A 2 motifs of ORF2 are found in the VP1u region, which is also the most conserved region among partetraviruses [19, 35] . The putative third ORF encodes a small protein containing a single transmembrane helix spanning 20 aa in the centre, with predicted molecular weights of 9.5 kDa in bovine partetravirus HK5 and 9.7 kDa in ovine parvovirus. The aa sequences of this putative protein are conserved among human, bovine, ovine and porcine partetraviruses, with pairwise sequence identities ranging from 59.3% (between porcine partetravirus HK7 and ovine partetravirus) to 96.4% (between bovine partetravirus HK1 and bovine partetravirus HK5).