Here we have investigated the entry route used by bluetongue virus serotype 1 (BTV-1) to infect BHK cells. Bluetongue virus (BTV) is the 'type' species of the genus Orbivirus within the family Reoviridae which includes many important pathogens for man and animals [37] . BTV exists as at least 25 serotypes [38, 39] and is the etiological agent of bluetongue, a severe and economically important haemorrhagic disease of ruminants. Bluetongue is arthropod-borne and primarily transmitted between susceptible animal hosts by certain species of Culicoides biting midges [40] .