For the convenience of experimental scientists, we give a step-by-step guide on how to use it to get the desired results as follows: (i) Open the web server AFP_PSSM [28] and you can see the prediction page on your computer screen, as shown in Figure 3 . You must input your email address since the prediction process may take a long time; (ii) Input your query protein sequence to the text box in Figure 3 . Note that the input protein sequence should be in the FASTA format. The FASTA format sequence consists of a single initial line beginning with a greater-than symbol (">"), followed by lines of amino acid sequence. You can click on the "example and note" button to see the example protein sequence; (iii) Choose a threshold value in the drop-down list. For prediction with high confidence (less probability of false positive prediction), high threshold should be chosen; (iv) Click on the submit button to see the predicted result. For example, if you use the first sequence in the example page, the predicted result will be "0.847538, yes" as can be seen in Figure 4 , which means that the protein is an antifreeze protein with the probability of 0.847538. It takes about 15 s for a protein sequence of 300 amino acids before the predicted result appears. 