Objective: It's expectable that the higher the number of prostate biopsies the greater the probability of prostate cancer detection, presumably improving the diagnostic accuracy and treatment decision. Method: Under this assumption, since May/2010, Santo António's Hospital implemented a prostate mapping system, increasing the number of biopsies performed from 8-12 to 16-18 and processing each core independently. We intend to establish a comparison between the traditional prostate biopsy system (TS) and the prostate mapping system (MS). For this study, all biopsy slides and corresponding surgical specimens from 60 patients subjected to MS and 60 patients subjected to TS were reviewed. Gleason score, percentage of tumor present in prostatic tissue (expressed as small ≤ 5 %, medium 6-19 % and large ≥20 %) and presence of tumor in prostate apex and base were analyzed. Results: Comparing MS with TS, the correlation coefficient was 53,5 % and 31,9 % for Gleason score and 46,9 % and 37,4 % for the percentage of tumor present in prostatic tissue, respectively. Mapped biopsies were able to detect the tumor in the apex and base in 72 % and 80 % of cases. Conclusion: These results suggest that prostate MS improves the diagnostic accuracy of prostate cancer and has a good ability to predict the presence of tumor in prostate apex and base.