Found at: doi:10.1371/journal.ppat.1000570.s001 (23 KB PDF) Figure S1 Protein network in mCMV. The protein interaction network was generated using Cytoscape software (www.cytoscape. org) [58] . Interactions previously reported in the literature are indicated with red edges. The colours of the nodes indicate in which herpesviral species a specific protein is conserved. [16, 17] . After each node is removed, the new network characteristic path length (average distance between any two nodes) of the remaining network is plotted as a multiple or fraction of the original parameters. The herpesviral networks consistently exhibited a higher attack tolerance, as the increase in path length is considerably smaller. Figure S13 Protein interaction partners of HSV-1 UL33 tested by Y2H. HSV-1 UL33 cloned in pGADT7 (prey) was tested against a variety of interaction partners cloned in pGBKT7 (bait). As negative controls, each bait was tested against the empty pGADT7 prey vector (left plates) while the UL33 prey was tested against the empty pGBKT7 bait vector (right plates). (A) Evaluation of mated yeast clones on double and triple selective plates with empty pGADT7 vector used as a control. (B) Evaluation of mated yeast clones on increasing amounts of 3-AT (0, 2.5, 5, 10 mM) with empty pGADT7 vector as a control. Self-activation of UL15, UL16 and UL21 at 0 mM 3-AT was suppressed at 5 mM 3-AT, while the interactions with UL33 were still found to be positive. Found at: doi:10.1371/journal.ppat.1000570.s014 (0.08 MB PDF)