In both diarrhea sample sets analyzed here, consisting of samples from adults, no association with HBoV2 shedding was detected. If HBoV2 causes diarrhea, it may do so in only a small subset of infected children, possibly those without passively transferred maternal antibodies or without protective immune responses from prior infections. Coinfections with other enteric viruses may also exacerbate symptoms. Given the very large number of childhood infections (viral shedding prevalence of 120% in some developing countries), even low virulence would translate into a large disease burden.