Streaks of Detrick 1, Detrick 2, and 1013 strains were grown for two days on TSA plates containing SBA.Bacterial cell mass was scraped using an inoculating loop (1 ml) from the streak and resuspended in PBS. Sporulation was evaluated by bright field phase-contrast microscopy. Phase-bright free sporesand phasedark vegetative cellswere counted. Five representative viewing fields were counted from each strain for each experiment. This experiment was completed in triplicate by repeating once per day over the course of three consecutive days.