We generated mRNA-seq libraries for sequencing on Illumina's GA Sequencer (San Diego, CA). 2 μg purified RNA was depleted of ribosomal RNA using Ambion's MICROBExpress Kit (Austin, TX) as per manufacture's recommended protocol. The enriched mRNA was used to prepare libraries using Illumina's Directional mRNAseq Library Prep v1.0 protocol. Briefly, 100 ng mRNA was fragmented with cations and heat, end-repaired, adapted by sequential ligation of unique 5-prime and 3prime adapters, reverse transcribed, PCR amplified, and purified using Agencourt's AMPure Beads (Beverly, MA). The libraries were visualized on an Agilent 2100 Bioanalyzer (Santa Clara, CA) and found to have the expected average fragment length of~250 bp.