Objective: Penile carcinoma (PC) is rare in developed countries, accounting for less than 1 % of all neoplasms in men, and biological characteristics of this tumor are poorly known. WNT and SHH pathways are important for cell proliferation, differentiation and survival, and therefore, play a role in carcinogenesis of various organs. The goal of this study is to investigate the expression profile of WNT and SHH pathway proteins in PC, characterizing the expression of target proteins from WNT (Wnt-1, Wnt-2, Wnt-2 Gsk3β, β-catenin, D1 Cyclin, MMP7, C-myc, CD44) and SHH (Shh, Smo, Gli, EGFR) pathways. Method: For that, 18 samples of PC were obtained from the files of Anatomic Pathology department from A.C. Camargo Hospital (Brazil) and submitted to immunohistochemistry. Results: We observed that Wnt-1 and Wnt-2 were expressed in 15 and 16 cases, respectively, out of the 18 evaluated samples. Strong Shh expression was detected in 16/18 cases, whereas weak and negative expression was seen only in one case each. Smo and Gli-1 proteins were expressed in almost all cases (17/18), and also D1 Cyclin, b-catenin and EGFR were frequently expressed. Conclusion: These preliminary results suggest that WNT and SHH pathways may be active and participating in the progression of penile cancer.