In 1929, Ciuca was first to use CFT for typing antiserum and FMDV of guinea pig origin [13] . Later, virus of bovine origin was successfully typed by CFT using guinea pig antiserum [23] . Since then CFT has been used extensively for distinguishing different strains of FMDV [24, 102, 103] . Subsequently, a modification of CFT, micro-CFT was developed, in which 96 well microtiter plates were used instead of tubes [104] . In the years 1964-1965 CFT (tube test) was used to replace the virus type identification by guinea pig cross-protection test [105] . Subsequently, the micro-CFT was adopted for this purpose [106, 107] . Although CFT was a fast method it needed high virus load and results were sometimes affected by pro-and anticomplementary activities of the test sample [26] .