Hepatitis B is one of the major diseases of mankind that kills about one million persons each year in the world. Accoring to primary study about 3% of Iranian population is chronic HBV carriers. Among Iranian cirrhotics, 70-84% has evidence of exposure to HBV and 51-56% is carriers. Because increase demand of blood transfusion, high blood dependent patients and long term window period of HBV infection, any controlling HBV infection program in blood donors can enhance the blood safety and public health.