Results: Here we describe a fast and sensitive method for the analysis of SSC in human urine samples using high-performance liquid chromatography (HPLC). The analysis is based on pre-column derivatization with ophthaldialdehyde (OPA) and separation on a C18 reverse phase column coupled to UV detection. Total analysis time of the described method is three minutes and quantification is linear in the range of 0.5 to 500 μM SSC. SSC values from 45 control pediatric and 43 adult urine as well as 60 plasma samples are reported.