The HBoV3 cluster included 11 strains, and the genomes of 2 representative strains, NI-374 and TU-A-210-07, were sequenced (GenBank accession numbers FJ973563 and FJ973562). When compared with HBoV2, the NS1 region of both strains showed an average amino acid divergence of 26% in the NS1 region and 9% in the VP1 region. Compared with HBoV1, the HBoV3 strains showed an average amino acid divergence of 12% in the NS1 region and 20% in the VP1 region ( Table 2 ). All 11 strains were classified as members of the HBoV3 species, recently described by Arthur et al [8] . A distantly related variant of HBoV3 was also recently identified in a sewage sample in the United States (HBoV3B-CA-1-C1; Figure 1 ) [36] . To confirm whether this variant represents a second genotype of HBoV3 will require full genome sequencing.