However in vivo evaluation of the present 3,5-diphenylisoxazole series has not been reported yet. [45] [46] [47] [48] [49] [50] [51] [52] [53] [54] [55] and also because 1,4-diphenyl-1,2,3-triazoles showed geometrical resemblance to furamidine. It was observed that the cytotoxicities of triazoles were as lower compared to that of pentamidine and were not affected by the alkylation on the amidine groups or the substitution on the aromatic rings. However, the placement of the cationic moiety in the 4-position increased the cytotoxicities of diamidines 46 ( Figure 15 ) with respect to the pentamidine. Except few, majority of unsubstituted diamidines exhibited higher in vitro activities against T. brucei rhodesiense than bis(N-isopropyl)amidines and diimidazolines.