Purified rNSs (2 mg) was incubated with polyclonal antibodies raised in rabbit against rNSs at 4uC for two hours. The rNSsantibody complex was precipitated by the addition of protein A sepharose beads. The ternary complex was separated by centrifuging the mixture at 5,000 rpm for two minutes and the supernatant was tested for ATPase activity.