Inhibition of proteins on HSV-1 antigen secretion A total of 1 × 10 5 VERO cells/mL were seeded into 24-well plates at 1 mL/well and then incubated with 5% CO 2 at 37°C for 24 hours. After abandoning the supernatant, 300 µL HSV-1 stock solution with 100 TCID 50 /mL was added to each well and incubated for 1.5 hours. Then, 100 µL of culture medium containing proteins (concentration was mentioned previously in the cytotoxity method) was added to each well and negative and positive cell control established. After incubation for 48 hours, HSV-1 antigen in culture supernatant was tested by human HSV-1 antigen 1 (HSV-1 AG1) enzymelinked immunosorbent assay kit with indirect ELISA: