PEM from WT or tlr2 2/2 C57BL/6J mice were infected with EGDe. Cells were lysed 0, 0.5, 1, 1.5, 2, 2.5, or 3 h post-infection. IkB and tubulin were detected in lysates by immunoblotting using anti-IkB (Santa Cruz, 1:100) and anti-a-tubulin (Sigma, 1:5000) antibodies.