and 4. The cloned CEACAM25 splice variants also code for proteins with only one N domain followed by a transmembrane domain. Three out of four clones encode cytoplasmic domains which contain the predicted ITAM motif (GenBank accession no. EF137908). In one clone, the absence of the 53 nucleotide cytoplasmic domain exon 1 leads to a frame shift and the usage of an alternative stop codon located in cytoplasmic domain exon3 (GenBank accession no. EF137909). Using supposedly CEACAM28specific primers, two products were amplified which differ in their length by 276 bp. Cloning and sequencing revealed that the CEACAM28 gene codes for a protein with one N domain, two A domains, a transmembrane domain and a cytoplasmic tail which contain an ITAM motif (GenBank accession no. EF137910). The sequence of the cDNAs cloned from the smaller PCR product was different from all genomic sequences published either for the boxer or the poodle breed and, therefore, was named CEACAM30. The differences are more or less randomly distributed within the whole coding region which consists of an N, A, transmembrane and cytoplasmic domain region. Two CEACAM30 splice variants were identified, both containing an ITAM motif in the cytoplasmic tail. They differ, however, in the usage of alternative splice acceptor sites of the cytoplasmic domain exon 1 (Gen-Bank accession nos. EF137911 and EF137912) ( Figure 4 ).