The preparation of multilamellar lipid samples is pretty much straightforward and only requires minor adjustments due to solvent compositions at high P/L ratios. In contrast, the requirements for the sample environment are rather high in terms of a stable and reproducible RH. Nevertheless, an advanced setup is not mandatory, since very distinct and stable RHs can also be generated by applying saturated salt solution reservoirs in a sealed sample chamber. (Greenspan 1977; Winston and Bates 1960) .