Another study evaluated the effect of quercetin on the parameters classically associated with alcohol liver injury, as lactate dehydrogenase (LDH), aspartate transaminase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in order to address the alterations of cell damage and antioxidant state after quercetin intervention; the ethanol-intoxicated (100 mM for 8 h) rat primary hepatocytes were simultaneously treated, pretreated (2 h) and posttreated (2 h) with quercetin. The toxic insult of ethanol on hepatocytes was challenged by quercetin and biochemical parameters almost returned to the level of control group when hepatocytes were treated with quercetin at the dose of 50muM for 2-4 h before ethanol exposure [24] .