As chickens are phylogenetically distant from mammals and have been shown to be refractory to T. gondii infection [38] , and differential recognition profile by antibodies from both classes of animals has already been described [39] . The comparative analysis of antigen recognition may be a useful tool to determine different virulence factors of the parasite, thorough proteomic studies for vaccine or diagnostic development. Moreover, the role of T. gondii antigens involved in parasite virulence has been largely addressed over the last decade [40] [41] . The most common protocols used for that purpose are the incubation of the parasite with specific antisera or the generation of strains with targeted depletions, preventing molecular interactions between host and parasite proteins [16] .