We examined these 536 regions, as well as the 394 conserved intergenic regions found by Gumby, to see if any of these showed significant expression in our logphase Mtb RNA-seq data. We calculated RPKM [108] values for each of these regions. We examined the regions with RPKM value ≥200 and a number of RNAseq reads ≥ 20. We eliminated an additional 35 regions which corresponded to known RNAs from the Mtb annotation, or RNAs similar to those found in M. bovis and Streptococcus [60] [61] [62] [63] , including 26 tRNAs, 2 riboswitches, and 3 found in other organisms.