The comparison of the transcriptome map of the H. somni genome with predicted proteins revealed the presence of frameshift mutations. Four genes have non-functional start codons, resulting in a predicted protein, truncated at the amino terminus (based on BLAST comparison with homologous proteins in other species), although full length mRNA was present. An example is presented for the gene ''HSM_0748'', annotated as ''Alpha-Lfucosidase'' ( Figure S1 ). The other three genes, HSM_0603, HSM_1666 and HSM_1668, encode a hypothetical protein, type III restriction protein res subunit, and CTP synthase, respectively. Two genes with frameshifts causing protein truncations (based on BLAST comparison with homologous proteins) are HSM_1385 (beta-hydroxyacyl dehydratase, FabA) and HSM_1744 (alcohol dehydrogenase zinc-binding domain protein). The transcriptome map revealed a full length mRNA for these two genes that code for truncated proteins.