Human brain tissue was kindly provided by the Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center. The Autopsy and Brain Donation procedures have been approved by the Duke University Institutional Review Board (IRB) and cortical brain tissue was obtained as described by [91] . 12 AD post-mortem confirmed cortical samples as well as 12 healthy cortical samples were obtained in dry ice. Cortical samples were of both genders, different ages, ApoE stati and Brack stages.