Principal component analysis (PCA) using the extended data analysis (EDA) module of the DeCyder software v7 revealed distinct clustering of the three experimental groups (FM, VM and HC) ( Figure 2B ). Proteins present in at least 80% of the spot maps, which passed the filter of one-way ANOVA (p,0.01) test were included in this multivariate analysis. Additionally, a hierarchical cluster analysis was performed using the same protein selection Figure 2C ). 85 protein spots were found to be significantly differentially expressed in the malaria subjects compared to the healthy individuals.