In particular, 28 peaks were confirmed on CM10, 19 on IMAC30, whereas none of the peaks could be validated on the surface H50. In the 3 fractions with different pH tested, F1 contained 28 peaks, F4 3 peaks, and F6 16 peaks. A higher number of peaks (n = 36) corresponded to small peptides (acquired at low focus mass 1-20 kDa), compared to big peptides (n = 11) that were acquired at high focus mass (Table 2 ). In line with the results of the discovery phase, the combination of peaks with the highest sensitivities (77% and 64.5%) and specificities (73% and 69.7%) were found on CM10 at low focus mass with the 14 discriminatory peaks of F1 and the 6 discriminatory peaks of F6, respectively ( Table 2 ). The correctly and incorrectly assigned piglets using these peaks are graphically illustrated in the heat map of Figure 1 ; part 1A shows the 14 peaks of F1 and part 1B the 6 peaks identified in F6.