HBoV detection has been mostly performed on NPAs and swabs and relies mostly on classical [1, 18, 19, 20, 21, 22, 23] and real-time PCR [24, 22, 25, 26, 27, 28] . Real-time PCR sure has advantage over the conventional PCR, as it offers greater sensitivity, specifity, and reduced expenditure of time. PCR assays detecting the NS1 or NP1 gene are most common. Tozer et al. established a highly sensitive real-time PCR assay targeting the NP1 and the VP1 gene and were able to detect HBoV in respiratory samples, as well as in fecal samples and whole blood [29] .