The area under the curve (AUC) values were computed by adding all OmniLog values at all time points for each of the 1200 distinct phenotypes produced from the OmniLog software. The AUC values from the two different biological replicates for each unique phenotype were averaged. The ratio for each AUC was calculated between the 6 query strains (Detrick-1, Detrick-2, Detrick-3, 1013-1, 1013-2, and Dugway) and reference parent strain (1942) . For the purpose of visualization, 1920 phenotypes were included in the heatmap (i.e. this better represents the locations of the phenotypes which correspond to different modes of action categories). The same ratios were used for the phenotypes that have replicates. The ratio values were formatted as PM1 to PM20 for each strain across the columns and wells A i to H i , where i = 1 to 12 for the rows. The results were plotted in a heatmap using R [25] . Positive growth wells are represented by greenblocks while negative growth wells are represented by red blocks.