Mouse Gr-1 + granulocytes from bone marrow were positively isolated using Anti-Ly-6G MicroBead Kit according to the manufacturer's protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Human neutrophils were isolated from discarded blood. This study was approved by the Institutional Human Subject's Review Board (City of Hope National Medical Center). Neutrophils were isolated by centrifugation over Ficoll-Paque Plus (GE Healthcare Biosciences, Pittsburgh, PA, USA) density gradient. Cell purities were determined by forward light-scatter/ side light-scatter gating of cells stained with PerCP-Cy5.5conjugated anti-CD16 mAb using a flow cytometer (FACSCanto TM II, BD Biosciences) and neutrophil purity was more than 95% [33] .