Pursuing our analysis on the putative involvement of DRMresident proteins in sperm function, the location of selected proteins associated with the light-density fractions was reanalyzed in sperm incubated under capacitating conditions. 7 . Schematic representation of the distribution of DRM proteins in sperm compartments depicting the results obtained using the antibodies directed against the different DRM-resident molecules analyzed in the study; HK1 localization as described previously [49] is also shown. As already noted in the text, IZUMO and FLOT2 demonstrate a different immunofluorescence pattern after the acrosome reaction (AR).