Conclusions: Over-expression of PEG10 can significantly promot L02 proliferation and ameliorate apoptosis-inducing effects of H2O2 on L02. Results: The PD-1 expression on Jurkat cells was induced by Hepatoma cells, the expression rate were 16.17±6.5% (by HepG2) and 17.43±6.8% (by HepG2 2.2.15), respectively. The cytokines IL-2 level (202.9±53.0 pg/ml), INF-level (88.6±4.6 pg/ml) and IL-10 level (63.7±13.4 pg/ml) in culture supernatant of blocking groups were significant higher than that of controls (IL-2, 102.9±53 pg/ml, INF-, 39.3±4.2 pg/ml and IL-10, 34.6±13.7 pg/ml, respectively. p < 0.05). The cytotoxic test (OD value) was markedly higher in blocking group (0.29±0.06) than that of control group (19±0.09 p<0.05) . Conclusion: The PD-1 expression on lymphocytes can be induced by Hepatoma cells, and cytokines expression and cytotoxic test were recovered by blocking PD-1/PD-L1 interaction. Background: Hedgehog (Hh) pathway is well known as a positive regulator for tissue construction( during development) and reconstruction (in adults). Our aim to observe the expression change of Hh pathway on rat hepatic regeneration . Materials and methods: Adult male Sprague-Dawley rats underwent approximately 70% partial hepatectomy (PH) or sham operation (SO). Liver specimens were collected at 2, 6, 12, 24, 36, 48, 72 , and 168h after PH or SO. Hedgehog expression was determined in mRNA level by RT-QPCR as well as in protein levels via immunohistochemical staining and western-blotting. Results: SO treatment did not induce remarkable changes in hedgehog expression; however, the level of transcript for hedgehog was significantly upregulated after PH. We found Sonic hedgehog(Shh )and Glioblastoma (GLi1-3) mRNA expression in the regenerating liver arrive at its peak at as early as 24 h and returned to its physiologyical level 168 h later. It is similar to the change of proteins (Shh and Gli1) .As seen from immunohistochemistry experiments; SHH protein was expressed uniquely in regenerating hepatocytes. Similarly, PH induced over expression for Shh protein occurred from 12 h with a peak level at 36 h after surgery. But Gli protein mainly located in nucleus and no significantly changes in the phrase of liver regeneration. Conclusion: Hedgehog pathway may play a role in the activation of hepatic proliferation during liver regeneration induced by physiological stress or pathological states, such as PH. Background: To investigate whether PEG10, an imprinted gene with an active paternal but silent maternal allele, was involved in hydrogen peroxide (H2O2) induced cellular apoptosis. Methods: PEG10 gene was stable transfected into L02. Cellular gene expression was determined by RT-PCR, Western blot and immunocytochemistry. Cell proliferation was analyzed by MTT. After treatment with different concentrations (50-400 mM) of H2O2, cell proliferation inhibition rate was measured by MTT. Morphological changes of apoptotic cells were determined by Hoechst33342 staining, DNA fragmentation was observed by agarose gel electrophoresis.