Overall, our findings suggest a differential prevalence of HBoV species in healthy individuals aged 0-70 years old. HBoV1 appears to be the dominant species responsible for HBoV infections among HBoV1, 2, 3, and 4 in Beijing, China. The seroprevalence of HBoV1-3 increased with age in children. Our study provides a basis for future evaluation of the epidemiology, genotype distribution, and pathogenesis of HBoVs worldwide.