In animals that were sacrificed on day 7 relative to the first infection, reductions in ALT and viral titers were most evident in the groups that received DEF201 within 24 h of infection ( Figure 6) . Notably, in the animals treated with the control rAd EV, there was an interesting trend that developed with the 6 h post-infection group having the greatest ALT levels and viral titers, followed by the 24, 48, and 224 h groups. This trend may suggest a low-level immune stimulation in the hamsters relative to the time at which the rAd EV was given. The resulting lack of measurable viral replication in the 224 h DEF201 group ( Figure 6B-D) is likely insufficient to elicit immunological memory. It is unclear as to why one of the first infection survivors from the 48 h rAd EV group ultimately succumbed to the second infection.