Lipid rafts have been implicated in regulating the clustering of nAChRs via the myristoylated peripheral membrane protein, rapsyn, which is constitutively lipid raft localized. Perturbation of the rafts impedes the interaction between rapsyn and nAChRs, which reduces clustering of the receptors and hence their function (Zhu et al., 2006) . Protein reconstitution studies using lipid vesicles imply that the partitioning of the nAChR into raft domains is not solely due to the intrinsic biophysical properties of the receptor but requires a signaling event to translocate the protein into specific membrane domains (Bermúdez et al., 2010) . Furthermore, cholesterol affects the properties, both structural and functional, of some acetylcholine receptors . Agrin signaling represents one pathway which can enhance the translocation of the nAChR into lipid rafts (Campagna and Fallon, 2006; Zhu et al., 2006) .