The normal mode of analysis is shown in Figure 5 . Here the cone voltage in the electrospray ion source is increased to 25 V increasing the level of fragmentation during ionization thus releasing the parent tag ion from the TNT-PNA conjugate. The parent tag ion is then selected from background by the first quadrupole in the Q-TOF instrument. The parent tag ion is subsequently subjected to CID in the second quadrupole of the Q-TOF instrument. A collision energy of 25 V was used for CID. MS/MS spectra showing the detected tag fragment ions are shown in Figure 5 . These spectra show ratios of two tags and demonstrate the accuracy of quantification of the TNT technology, which is discussed in the next section.