RV with T. esculentum Extracts. Tylosema esculentum ethanolic extracts, namely, seed coat ethanolic extract (MSCE) and cotyledon ethanolic extract (MCE) were dissolved using a rotary magnetic stirrer (Yellowline, BigSquid, Kika-Werke GMBH & Co, Germany) in DMEM (Sigma-Aldrich, Grand Island, USA), supplemented with 10% foetal calf serum (Cambrex, Verviers, Belgium), L-glutamine (2 mmol/L, Sigma), penicillin (100 units/mL, Sigma), and streptomycin (1 mg/mL, Fluka, Buchs, Switzerland). Rotavirus at 1 × 10 7.5 /mL TCID 50 was combined with concentrations of T. esculentum extracts (see Table 2 ). As controls, DMEM (Sigma-Aldrich, Grand Island, USA), supplemented with 10% foetal calf serum (Cambrex, Verviers, Belgium), Lglutamine (2 mmol/L, Sigma), penicillin (100 units/mL, Sigma), and streptomycin (1 mg/mL, Fluka, Buchs, Switzerland), dilutions of Combivir GlaxoSmithKline-150 mg lamivudine and 300 mg zidovudine (AZT) as antiviral agent as described by Agelis et al. [32, 33] and T. esculentum extract at below IC 50 concentrations and RV solutions at 1 × 10 7.5 /mL TCID 50 were separately used. The plant extract and virus, Combivir, and virus mixtures were added to monolayers of cells in 96-well plates and then maintained at 37 • C in atmosphere of 5% CO 2 for 24 h.