Two of the TTSuV-1 sequence reads partially overlapped and the sequence analysis indicated a significant variation between the two and therefore two different TTSuV-1 PCR assays were designed. The results from the specific PCR assays showed that one of the TTSuV-1 variants (here named TTSuV-1a) could be found in two of the three bushpig sera while the other one (here named TTSuV-1b) was found in all three. The sequence analysis of the PCR amplified TTSuV-1 products (Gen-Bank accession number JQ277338 -42) showed a sequence similarity between TTSuV-1a and b on protein level at 53-54.5% in the analysed 66 amino acid region. Compared to sequences from other studies, TTSuV-1a showed a 60.6-84.8% protein sequence similarity while TTSuV-1b was more divergent (50-56% similarity). These protein sequence identity values were similar to those seen when comparing the sequences retrieved from the GenBank with each other (59-100% similarity). The phylogenetic tree (Figure 2 ) also indicated that TTSuV-1b was more divergent than the other sequences. Sequences from different parts of the world such as China, Germany, Spain, US etc. was used in the phylogenetic study however no clear geographical clustering was seen.