The intracellular distribution of DV1 E glycoprotein was analyzed in fixed HeLa-prME cells by co-labeling with Erp72, ERGIC-53 and Golgin-97, which are proteins of the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment (ERGIC) and the Golgi apparatus, respectively. As expected, the DV1 E glycoprotein mainly distributed in the ER in HeLa-prME cells, as shown by immunofluorescence, where it colocalized with Erp72 ( Figure 2A -D), whereas it showed no colocalization with ERGIC-53 ( Figure 2E -H) and Golgin 97 ( Figure 2I -L). Interestingly, E and Erp72 were also enriched in a perinuclear compartment, which is not usually labeled by anti-Erp72 antibody and other ER markers (data not shown), suggesting that it has been induced by prM and E viral protein expression. In addition, no staining was observed at the plasma membrane, confirming the previous flow cytometry result. Altogether, our data demonstrate that the DV1 E glycoprotein is efficiently expressed and enriched in the endoplasmic reticulum in the HeLa-prME stable cell line.