The primary screens additionally identified 19 essential host genes that inhibited BMV-directed Rluc expression at least 6-fold in both passes at the same time point (Table 2 ). Northern blotting confirmed statistically significant decreases in BMV (+)RNA4 accumulation for 5 of 19 genes (,26% confirmation) at a false Table 2 . Genes whose repression was associated with $6fold inhibited BMV-directed Rluc expression in both primary screen passes. discovery rate of 5% ( Fig. 3 and Table 5 ). (+)RNA4 levels were inhibited ,1.5-to 8-fold in these strains ( Fig. 3 and Table 5 ). In addition to the severe inhibition of (+)RNA4 accumulation in P TET -HSF1, P TET -PRE1, and P TET -RPT6, (2)RNA3 was inhibited 5.5-, 1.8-, and 5.5-fold in these strains, respectively ( Fig. 3 and Table S6 ). Interestingly, all five genes that inhibited BMV RNA replication (ESS1, HSF1, JAC1, PRE1, and RPT6) perform cellular functions that, in various ways, contribute to modulating host protein levels ( Table 4) .