To test whether the phenotype observed on blood agar was associated with differences in sporulation, selected strains were grown for two days as patches on blood agar, resuspended in PBS Annotations are a combination of RAST and directed tBLASTn and BLASTp searches vs Bacillus databases. 4 Forms part of a large polypeptide synthase containing highly homologous regions. 5 Also shared with strain ATCC 49822. 6 The conserved start codon of the radA gene (BG3899) of B. atrophaeus and B. subtilis falls within the BG3898 ORF. Therefore BG3898 as called by RAST is not deemed likely to be a protein-coding gene. 7 In putative transmembrane region. doi:10.1371/journal.pone.0017836.t004 Table 4 . Cont.