In the present study, we produced and purified polyclonal anti-T. gondii antibodies from STAg-immunized hens. These anti-STAg antibodies were obtained from the egg yolk, in a protocol which animal bleeding turned out to be unnecessary [19] . Generally, yolk IgY arise around 7 th to 10 th day after the sera antibodies, in a sera concentration-dependent manner [20] . In addition, the large amounts of polyclonal antibodies recovered from the egg yolk in this process, the high avidity of the produced IgY antibodies, and their potential applications are itself the great features of the protocol herein described. Egg yolks contain large amounts of IgY passively transferred by hens, with mass ranging between 2-8 mg per mL after purification, and it has been estimated that 2-10% of total egg yolk antibodies are antigen-specific [21, 9] . To obtain STAg-specific IgY we used simple protocols, which are easily reproduced without the need of refined equipment or reagents. Additionally, installations required for IgY production are smaller and easier to handle than those designed for small ruminants, commonly used for large scale antisera production [22] . The production of specific IgY has been previously described for the recognition of a broad range of targets, including Plasmodium falciparum [23] , hepatitis A virus [24] and tumor cells [25] [26] .