Primary cell culture of bovine [108] [109] [110] , ovine and porcine [111] origin has exhibited susceptibility to FMDV from infected tissues. However, the most sensitive culture system for virus isolation is primary bovine thyroid cells [109] but cryopreservation of bovine thyroid cells directly after trypsinization results in the loss of susceptibility to FMDV [110] . Some stable cell lines, like IBRS-2 [112] , MVPK-1 clone 7 [112, 113] , LFBK cell line and Veterinary Medicine International 5 BHK-21 [114] are also susceptible to FMDV and so are most desirable for diagnostic system but these are less sensitive than primary cells for detecting low amount of infectivity [115] .