Plated monocytes were transduced as previously described [56] . In brief, freshly isolated monocytes were transduced with VSV-G pseudo-typed SIV VLPs and each lentiviral vector construct for 3 h by spinoculation, in the presence of 2 mg/mL polybrene (Sigma). Subsequently, cells were washed, resuspended in regular growth medium described before for the generation of monocytes derived human dendritic cells and incubated for 5 days at 37uC until stimulation. At day 5 post-transduction, MDDCs were infected with DENV as described before, and cell pellets were collected at the indicated time points, and lysed for RNA isolation.