Confocal Microscopy of Proteins Identified by SILAC as Having No Change in Nucleolar Abundance at 18 h Postinfection-For completeness, we tested a protein that did not change its location at this time point postinfection. Nop132 is a nucleolar antigen that has not been previously investigated in the context of adenovirus infection. We used a FLAGtagged construct to examine its location in uninfected and infected cells. As can be seen from Fig. 6 , Nop132 maintained its apparent nucleolar location with no detectable depletion from the nucleolus to other compartments.