For identification of peptides presented by MHC class I that can be used in ELISPOT and pentamer staining experiments we used the software programs SYFPEITHI (University of Tübingen, Germany) and BIMAS (BioInformatics and Molecular Analysis Section, National Health Instituts, Bethesda, USA) to establish a ranking of potential peptides. From 12 potential H-2 K b and D bpresented peptides of MV-N and MV-H with the highest probability scores, we found that D b MV-H 22-30 and D b MV-H 446-454 (RIVINREHL and SNHNNVYWL, respectively) were most efficiently recognized. ELISPOT experiments were performed using the Mouse IFN-c ELISPOT set (BD Biosciences) according to the manufacture's protocol.