We previously demonstrated that 9 out of 11 subtype C infected subjects from the ZEHRP cohort developed robust autologous Nab responses against the infecting Envs, with 50% inhibitory (IC50) titers often exceeding 1:3,000 within the first few months of infection [15] . For two of the subjects who developed potent autologous Nab and were identified as viral p24 antigen positive (Table 1) , we sampled the emerging quasispecies by single genome PCR amplification, cloning, and sequencing of biologically functional env genes from longitudinal plasma and PBMC DNA samples. For both subjects, the 0-month Envs were cloned at the first seropositive time point estimated to be within 48 days of infection, and longitudinal timing was calculated in months from