Several hMAbs from donor 013 bound to EDIII (Figure 3) . Two approaches were used to map the binding sites of these hMAbs on EDIII. Two strongly neutralizing hMAbs that bound EDIII, which were type-(DV3.7) or subcomplex-(DV10.16) specific, were mapped by identifying neutralization escape variants after passaging DENV2 in the presence of each antibody (ired a mutation on EDIII. The virus passaged in the presence of hMAb DV3.7 acquired the single point mutation V382G (Table 3 and Figure 4C ). This residue is located on the EDIII lateral ridge, which is a target of previously mapped type-specific strongly Human Antibody Response to Dengue Infection www.plosntds.org neutralizing mouse MAbs [17, 23] (Table 3 and Figure 4C ). The virus passaged in the presence of hMAb DV10.16 acquired the point mutation E311K (Table 3 and Figure 4C ). This amino acid is located on the A strand of EDIII and forms part of a dengue subcomplex epitope recognized by neutralizing mouse MAbs [18, 23] (Table 3 and Figure 4C ).