The 24 sequences from H18 were assembled to form a continuous sequence of approximately 2700 nucleotides that appeared to lack .1300 and 1000 nucleotides from the 59and 39 ends of its genome. PCR primers based on the available H18 sequences and regions highly conserved between HBoV2 (FJ170278) and canine bocavirus (FJ214110) were used to amplify the nearly complete bocavirus genome we provisionally called PBoV1-H18 (5267 nt). To confirm this genome sequence, this sequence was re-amplified using 6 sets of PCR primers generating overlapping fragments of the genome which were directly sequenced. Using the same method, the nearly complete bocavirus genome (5117 nt) from sample A6 was also sequenced and was provisionally labeled PBoV2-A6.