Localization Patterns of Sequestered Cellular Antigens Are Heterogeneous-Interestingly, there was a diverse range of localization changes in response to infection. For example, eIF6 was diffuse throughout the infected nucleoplasm. Alternatively, hnRNPU, SFPQ, and U2AF 65 were adjacent to DBPrich centers, whereas histone H1c was cleared from the DBPrich centers and regions adjacent to the DBP centers. This suggests that exclusion of cell proteins from DBP-rich centers is not simply a nonspecific feature of the adenovirus-infected cell nucleoplasm. Moreover, hPOP1 was excluded from the DBP-rich centers but was clearly enriched in a mottled pattern distinct from Nopp140 and U2AF 65 for example. Thus, there is, as might be expected, enrichment of nucleolar antigens in distinct regions of the infected nucleoplasm. This suggests, not surprisingly, that distinct aspects of adenovirus replication benefit from different nucleolar antigens.