Cytopathic effects of RV alone and in coculture with T. esculentum extracts or Combivir added at 0.75 mg/mL {modified from Agelis et al. [33] } on cell monolayers after treatment as listed above were measured after 24-hour coincubation of cell monolayers (1 × 10 6 cells/mL) at 37 • C in atmosphere of 5% CO 2 . It was previously shown that T. esculentum extracts alone were not cytotoxic at the concentrations used in these tests; hence the observed reduction in survival of cells upon coincubation of extract RV solutions was attributed mainly to the virus. Following incubation, relative viability of cells was determined photometrically in terms of amount of crystal violet incorporated in viable cells as described previously.