IL-6, IL-10, TNF, IFN-c and MCP-1 levels were determined in lung homogenates using a cytometric bead array technique (BD Cytometric BEAD Array Mouse Inflammation Kit; BD Bioscience, San Diego, CA, USA) according to the manufacturer's instructions. Briefly, 50 ml mouse inflammation capture bead suspension and 50 ml PE detection reagent were added to an equal amount of sample standard dilution and incubated for 2 h at room temperature in the dark. Subsequently, samples were washed by adding 1 ml wash buffer and centrifugation at 2006 g at room temperature for 5 min. Supernatants were discarded and 300 ml wash buffer was added. Samples were analyzed on a BD FACSArray bioanalyzer (BD Bioscience) according to the manufacturer's instructions. Standard curves were prepared similar to the method above. Data were analyzed using BD CBA Software (BD Bioscience). Finally, the chemokine or cytokine levels were recorded as pg/ml homogenate.