The original P1-VP2 sequences described in this study were deposited in GenBank under accession nos. GQ223119 to GQ223136. The VP4-VP2 sequences were deposited under the nos GQ223137 to GQ223181, and the P1-P2 sequences under the nos GQ223182 to GQ223226. The full length genomes sequences of N4, N10 and N13 strains were deposited under the nos. GQ223227, GQ223228, GQ223229, respectively. Poster Exhibition  Background: Biliary atresia (BA) is one of the most common causes of neonatal cholestasis and the most frequent hepatic cause of death in early childhood. The incidence rate of BA is higher in Asian countries, occurring in approximately 1 of 8,000 (Asian Countries ) to 1 of 18,000 (European countries) live births. Early identification and prompt intervention is very important. To im prove the early diagnosis, we used proteomic technology to screen serum biomarker for BA. Methods: Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption /ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were employed to screen serum biomarkers specific to BA sera from idiopathic neonatal hepatitis. After pretreatment including albumin and immunoglobulin (IgG ) depletion, sera were subjected to 2-DE and there after image analysis. The differentially expressed protein spots were identified by MALDI-TOF-MS. Result: From optimized 2-DE gel images, thirty-four spots were differentially expressed and identified by MALDI-TOF-MS to be eight proteins. Overall, kininogen 1 variant was under expressed and alpha-1-B-glycoprotein, leucine-rich alpha-2-glycoprotein 1, 'SP40,40', A1BG protein, vitamin D-binding protein/group specific component , apolipoproteinA-, AQGV 3103 were over expressed in BA group com pared to idiopathic neonatal hepatitis. Conclusion: 2-DE based serum proteome analysis can be useful in detecting protein expression alteration and new discovered biomarkers might be an aid in the diagnosis of BA, though further validation is needed.