In the second set of experiments, two groups of five mice were immunized at 8 weeks of age with a single 200 ul intraperitoneal injection of the following: (E) 50 ug of p163 in incomplete Freund's adjuvant (IFA); or (F) IFA alone. Mice were serially observed and at 36 weeks of age, they were sacrificed. Sera were assayed for autoantibodies. The livers were removed for evaluation as described above.