Lentiviral vector constructs were built using conventional molecular biology techniques. Briefly, human STING cDNA was PCR amplified from pcDNA3.1 hSTING [13] and cloned into a lentiviral vector derived from pHR SIN CSGW [54] ( Figure 5G ). Mutations in the NS2B3 cleavage site at positions 94-96 of hSTING were obtained by overlap PCR. Residues RRG were changed to the corresponding murine sequence HCM.