Methods: 1507 subjects were selected in Gongcheng city of Guangxi Zhuang Autonomous Region, and the clinical trail was carried out according to the random, double-blind and parallel principle from January to August, 2005. After vaccination by 0, 6 schedule, adverse events of the subjects were observed, the seroeonversion rate and geometric mean titer (GMT) were tested by the competitive inhibition ELISA. Results: After immunization, the systemic and local reaction rates of adults were 8.80% and2.67%, which was no significantly statistical difference compared with control group, 12.41%and 4.41%; while the rates of children were 10.60and 2.28%%, and no significant statistical difference compared with control group, 10.71%and2.86%. One month after first dose of vaccination, the seroconversion rates of children and adults were 88.2% and 93.8%, and one month after second dose of vaccination, the rates were all 100%, the GMTs of children and adults were 16447mIU/m1 and 8555mIU/ml, which was significant statistical difference in children compared with control group, 1946 mIU/ml and 5881 mIU/ml, respectively.