Significant IL-10 responses were also detected following boosting with PyCSP IVTT purified using either NI-NTA resin (p,0.001) or MagneHis Ni-particles (p,0.001), as compared to Alum control, but not following boosting with unpurified whole extract or IVTT products associated to either Polybeads or ProteinG beads, or with PyCSP DNA ( Figure S2C ). TNF-a responses were not significantly different from the controls for any of the conditions ( Figure S2D ). IL-2 responses were induced only by purified IVTT products (data not presented). No significant responses were detected for other cytokines assayed using the CBA assay.