When the results of the new NucZip scoring system were scored against the % MFI in Figure 6A , which shows all the target and probe combinations plotted in Figure 6B , it was found that they were more highly correlated with the experimental data than the predicted ÁG. To investigate these differences, each outlier in Figure 6B was connected to its plot position in Figure 6A ; see Figure 7A and B. Figure 7 shows that probe-target combinations containing many mismatches and dInosines were the main causes of the lower correlation between predicted and observed hybridization in Figure 6B . However, hybridizations between a long probe and a short target were not included in Figures 6  and 7 . Nor were data from probes containing 5-nitroindole or N-wobbles, because a full investigation such as this would require many more observations and would be out of the scope of this article. The NucZip results are further discussed in the Discussion section.