The isolation chamber working zone is composed of a stainless-steel box and two front doors. The welded box was manufactured using dumb-gloss stainless-steel 316L with a thickness of 3 mm. The interior chamber dimensions are 400 cm (L)×120 cm (W)×120 cm (H). The isolation chamber front door includes two damping braces on each front side with a dual-piston mechanism for holding the front door securely open to let the animal cages in, two operation panels with stainless-steel 316L framework, and two door hinges connected to the stainless-steel box. The operation panel is made of transparent polymethylmethacrylate (PMMA). The transparent front door allows for visualization of the contents of the isolation chamber. Silicone seals around the PMMA panel ensure that the system is air tight. The front door is manually fastened onto the box framework with a hammer bolt. There are seven circular polyethylene (PE)-machined glove ports on the operation panels. The port inner diameter is 300 mm and the center-tocenter spacing of each port is 450 mm. The glove port assembly includes a glove port ring, glove port gasket, pressure ring, and glove port inner-securing ring. The glove port ring and glove port inner-securing ring are jointed with a thread connection. The glove port ring edges are fixed on the PMMA panel by compressing the glove port gasket and pressure ring on each side of the PMMA panel by tightening a fixing screw.