Genotyping and assessment of viral load in HCV patients are vital for designing therapeutic strategies. We aimed to determine the pattern of HCV genotypes and its association with viral load and biochemical profile. Methods: 71 HCV RNA positive patients were included in the present study attending the medical-OPD and wards of Dr RML Hospital, a tertiary care hospital in New Delhi during 2006-2008. HCV genotyping was carried out by restriction fragment length polymorphism (Buoro et al 1999) followed by the type specific primers from the core region (Ohno et al 1997) . Viral load estimation was carried out by Taqman real time PCR system using previously described method (Martell et al 2000) . Result: 67.6% of cases were having genotype 3 (3a, 3b, 3f & 3i) followed by genotyping 1 (1a & 1b) in 26.8% and genotype 2 in 5.6%. There was no statistical significant difference seen in the biochemical profile between the three groups of genotypes. Genotype one was associated with a significantly higher viral load as compared to the genotypes three and two. Parentral mode of transmission was accounted for the 68% of all the infected cases. Conclusion: HCV genotypes 3 and 1 accounted for 94% of our cases. The genotype 1 is associated with higher degree of disease severity as assessed by viral load. Also two unusual subtypes 3i and 3f were identified from this geographical region.