The effects of cancer treatment can be desired, i.e. a treatment response or undesired, i.e. complications which may harm or even kill the patient. Complications are classified as acute, defined as those encountered up to 3 months; sub-acute, between 3 and 12 months; and delayed or late complications after 12 months. However, there is variation in susceptibility among individuals and there may be overlap of the acute, sub-acute and early delayed effects in the first few weeks to months following treatment. Radiological manifestations are often non-specific and thus the clinical context and temporal relationship to induction of therapy are important considerations. Good communication between the radiologist and the oncologist is vital for the early recognition and accurate diagnosis of complications related to treatment. The role of the radiologist is to help clinical colleagues to make a distinction between the effects of the cancer and the effects of its treatment so that ineffective or toxic therapies can be discontinued. Cancer patients with treatment complications usually present with common and non-specific symptoms such as Discovery and Targeted LC-MS/MS of Purified Polerovirus Reveals Differences in the Virus-Host Interactome Associated with Altered Aphid Transmission Circulative transmission of viruses in the Luteoviridae, such as cereal yellow dwarf virus (CYDV), requires a series of precisely orchestrated interactions between virus, plant, and aphid proteins. Natural selection has favored these viruses to be retained in the phloem to facilitate acquisition and transmission by aphids. We show that treatment of infected oat tissue homogenate with sodium sulfite reduces transmission of the purified virus by aphids. Transmission electron microscopy data indicated no gross change in virion morphology due to treatments. However, treated virions were not acquired by aphids through the hindgut epithelial cells and were not transmitted when injected directly into the hemocoel. Analysis of virus preparations using nanoflow liquid chromatography coupled to tandem mass spectrometry revealed a number of host plant proteins co-purifying with viruses, some of which were lost following sodium sulfite treatment. Using targeted mass spectrometry, we show data suggesting that several of the virus-associated host plant proteins accumulated to higher levels in aphids that were fed on CYDV-infected plants compared to healthy plants. We propose two hypotheses to explain these observations, and these are not mutually exclusive: (a) that sodium sulfite treatment disrupts critical virion-host protein interactions required for aphid transmission, or (b) that host infection with CYDV modulates phloem protein expression in a way that is favorable for virus uptake by aphids. Importantly, the genes coding for the plant proteins associated with virus may be examined as targets in breeding cereal crops for new modes of virus resistance that disrupt phloem-virus or aphid-virus interactions. Virus species in the Luteoviridae, including Cereal yellow dwarf virus (CYDV)-RPV, referred to herein as luteovirids, are each exclusively transmitted by one or a few species of aphids in a persistent, circulative, non-propagative manner. Aphids can transmit acquired virus for days or weeks and even after molting [1, 2, 3] . Virus is acquired when the virus moves from the aphid hindgut (HG) lumen to the hemolymph. Once in the hemolymph, the virus passes into the accessory salivary gland (ASG) and is released into the salivary duct. Aphids transmit the virus into phloem cells during salivation and feeding. Through specific interactions with putative cellular receptors [4, 5, 6] , luteovirids actively cross the membranes of the aphid HG and ASG. The HG and the ASG are the major barriers to the successful aphid acquisition and transmission of luteovirids. The movement of luteovirids across these barriers is under genetic control by an unlinked set of aphid genes [7] that are additive in effect [8] . These genes encode for proteins that are differentially expressed in aphids with varying virus transmission efficiency [9, 10] .