Calorie restriction (CR) extends the lifespan of many organisms [33] . The identification of CR mimetic compounds may therefore enable therapeutic retardation of the onset of various age-related disorders, including metabolic syndrome, cancers, neurodegenerative diseases, and other rare disorders [34] . The development of CR mimetics would therefore fulfill unmet medical needs. Although SIRT1-activating compounds (STACs) may offer a promising approach to the development of CR mimetics, a novel approach to finding potential CR mimetics is required [9] . For this purpose, we developed a cell-and mouse-based screening system using an extracellular reporter assay [10] .