In this paper we show that expression in vitro of the murine norovirus VP2 protein occurs by coupled translation terminationreinitiation. The process requires the close proximity of stop and start codons, a defined region of mRNA upstream of the stop-start window that includes a functional TURBS Motif 1 and translation by the ribosome through this region up to the site of terminationreinitiation. Secondary structure mapping indicates that the RNA in this region is weakly structured, with Motif 1 loosely embedded in the 59 arm of a putative stem-loop structure. The MNV signal thus exhibits many of the features and functional characteristics of the stop-start signals of FCV, RHDV and influenza B. The Figure 1 ] which acts as the ''wild-type'' reference construct [WT] in these experiments), in which the stop and start codons of the termination-reinitiation signal were altered. The figure shows the primary sequence and three-frame translation of the relevant region of the mRNA encoded by each construct. The natural stop-start motif is shown in pink and emboldened text, the downstream fortuitous stop-start motif in pink. Mutations within the mRNA sequence are highlighted by uppercase, red emboldened characters. The upstream rlucVP1 ORF is highlighted in grey, as is the downstream VP2fluc ORF where this is known.