DNA sequencer
Comparison

DNA sequencer

Section Title: Comparison
Table Section Text: None

Sequencer	Ion Torrent PGM	454 GS FLX	HiSeq 2000	SOLiDv4	PacBio	Sanger 3730xl

Manufacturer	Ion Torrent (Life Technologies)	454 Life Sciences (Roche)	Illumina	Applied Biosystems (Life Technologies)	Pacific Biosciences	Applied Biosystems (Life Technologies)

Sequencing Chemistry	Ion semiconductor sequencing	Pyrosequencing	Polymerase-based sequence-by-synthesis	Ligation-based sequencing	Phospholinked fluorescent nucleotides	Dideoxy chain termination

Amplification approach	Emulsion PCR	Emulsion PCR	Bridge amplification	Emulsion PCR	Single-molecule; no amplification	PCR

Data output per run	100-200 Mb	0.7 Gb	600 Gb	120 Gb	0.5 - 1.0 Gb	1.9∼84 Kb

Accuracy	99%	99.9%	99.9%	99.94%	88.0% (>99.9999% CCS or HGAP)	99.999%

Time per run	2 hours	24 hours	3–10 days	7–14 days	2–4 hours	20 minutes - 3 hours

Read length	200-400 bp	700 bp	100x100 bp paired end	50x50 bp paired end	14,000 bp (N50)	400-900 bp

Cost per run	$350 USD	$7,000 USD	$6,000 USD (30x human genome)	$4,000 USD	$125–300 USD	$4 USD (single read/reaction)

Cost per Mb	$1.00 USD	$10 USD	$0.07 USD	$0.13 USD	$0.13 - $0.60 USD	$2400 USD

Cost per instrument	$80,000 USD	$500,000 USD	$690,000 USD	$495,000 USD	$695,000 USD	$95,000 USD

Sentence(s)

These include the 454 and SOLiD DNA sequencing platforms.
DNA sequencer includes the 454, and SOLiD sequencing platforms.

final_sentence: These include the 454 and SOLiD DNA sequencing platforms.
final_sentence: DNA sequencer includes the 454, and SOLiD sequencing platforms.