I list the peer reviewed publications that support the information that the use CRUSIO continues to remove is shown below. The name of the system that was developed in Dr. Timiras lab is called the Automated Imaging Microscope System and not the automated tissue image analysis system, that the user CRUSIO continues to call it. Please refer to the PUBMED publication at http://www.ncbi.nlm.nih.gov/pubmed/15990721?dopt=AbstractPlus to verify the name in the 4th sentence of the Abstract. Please verify the information related to CREA at the CREA web site http://crea.berkeley.edu/paul_segall_profile.shtml and http://crea.berkeley.edu/crea.shtml. I would be very happy to discuss these references with you and information with you.

Estrogen receptor-alpha immunoreactivity in the arcuate hypothalamus of young and middle-aged female mice; 
Yaghmaie F, Saeed O, Garan SA, Voelker MA, Sternberg H, Timiras PS; 
Neuro Endocrinol Lett. 2010 Feb 16;31(1):15.

Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice; 
Yaghmaie F, Saeed O, Garan SA, Voelker MA, Gouw AM, Freitag W, Sternberg H, Timiras PS.; 
Neurology & Neurosurgery, Para. 488, Page 99, Sec. 8, Vol 152.2, 2007

Tracking changes in hypothalamic IGF-1 sensitivity with aging and caloric restriction; 
F. Yaghmaie, O. Saeed, S.A. Garan, A.M. Gouw, P. Jafar, J. Kaur, S. Nijjar, P.S. Timiras, H. Sternberg and M.A. Voelker; 
Experimental Gerontology, Volume 42, Issues 1-2 , January-February 2007, Pages 11-12.

Insulin-like growth factor-1 receptor immunoreactive cells are selectively maintained in the paraventricular hypothalamus of calorically restricted mice; 
Saeed O, Yaghmaie F, Garan SA, Gouw AM, Voelker MA, Sternberg H, Timiras PS.; 
Int J Dev Neurosci. 2007 Feb;25(1):23-8

Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice; 
Yaghmaie F, Saeed O, Garan SA, Voelker MA, Gouw AM, Freitag W, Sternberg H, Timiras PS.; 
Int J Dev Neurosci. 2006 Nov;24(7):431-6.

A study of insulin-like growth factor-I receptor immunoreactivity in the supraoptic nucleus of young and old female B6D2F1 mice; 
Farzin Yaghmaie, Omar Saeed, Steven A. Garan, Arvin M. Gouw, Tien Tran, Jacqueline Ho, Liu Y. Zhao, Mark A. Voelker and Paola S. Timiras; 
FASEB Journal, April 2006, 357.5

Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice; 
Yaghmaie F, Saeed O, Garan SA, Freitag W, Timiras PS, Sternberg H; 
Neuro Endocrinol Lett. 2005 Jun;26(3):197-203.

A survey of estrogen receptor-alpha immunoreactivity in the hypothalamus of young, old, and old-calorie restricted female B6D2F1 mice;
F. Yaghmaie, S.A. Garan, O. Saeed, W. Freitag, P.S. Timiras, H. Sternberg, M. Voelker; 
Experimental Gerontology, Volume 39, Issues 11-12, November-December 2004, Page 1771

A comparison of estrogen receptor-alpha immunoreactivity in the arcuate hypothalamus of young and middle-aged C57BL6 female mice;
Yaghmaie F, Garan SA, Massaro M, Timiras PS; 
Experimental Gerontology, Volume 38 (2003), Issues 1-2, Page 220

Creating Three-Dimensional Neuronal Maps of the Mouse Hypothalamus Using an Automated Imaging Microscope System; 
Garan SA, Neudorf J, Tonkin J, McCook LR, Timiras PS.; 
Experimental Gerontology, Volume 35 numbers 9-10, December 2000, page 1421

Automated Imaging Microscope System; 
Jason Neudorf and Steven A. Garan; 
Linux Journal 2000, Issue 70, Februrary, Page 32-35