BACKGROUND
parkinson disease  is a common and progressive neurological disease that is estimated to afflict 1 % of all individuals over the age of 60 years worldwide  <cit> .
although the direct cause of pd is as of yet unknown, the clinical symptoms include resting tremor, bradykinesia, rigidity and postural instability.
in addition to these classical symptoms recent discoveries have shown that effects may include cognitive impairment such as loss of memory and depression  <cit> .
these symptoms are caused by a decrease in the amount of dopamine available in the striatum area of the brain and in many cases characterized by an accumulation of harmful protein aggregates known as lewy bodies in the neurons of the substantia nigra pars compacta  <cit> .
the eventual dysfunction and breakdown of these neurons is responsible for the symptoms and pathology of pd  <cit> .
both genetic and environmental factors have been found to contribute to pd with many forms of the disease being attributed to a combination of the two.
environmental factors include: chemical exposure, brain trauma, obesity, diabetes and age  <cit> .
alternatively, there have been a number of familial cases of pd identified which suggests a genetic link to certain forms of this disease  <cit> .
this link is especially strong in the case of early onset pd which shows a bias towards genetic causes, while environmental conditions seem to more often than not be linked to late onset pd  <cit> .
identifying and analysing the genes responsible for these inherited forms of pd may give rise to mechanisms of disease progression that are not presently understood.
impaired neuronal activity has been shown to contribute to the lack of dopamine commonly associated with pd etiology.
identifying the cause of this neuronal impairment may help lead to new, effective, strategies to combat pd.
as important and necessary components of eukaryotic cells, mitochondria are integral components in the creation of adenosine tri-phosphate  for use as chemical energy and are involved in other important pathways such as the control of cellular growth and death, cell signalling and differentiation  <cit> .
as the mitochondria are involved in such diverse and important cellular functions; it is not surprising that the breakdown or dysfunction of mitochondria may result in a number of disorders or diseases including but not limited to movement disorders such as pd  <cit> .
many of these disorders are caused by defective removal of damaged or non-functional mitochondria and a subsequent lack of de novo synthesis of new mitochondria to replace the aforementioned damaged organelles.
thus, the study of genes that regulate and monitor the health of mitochondria is a reasonable next step for determining potential disease prevention strategies.
the peroxisome proliferation activated co-receptor gamma  family of genes have been linked to mitochondrial biogenesis  <cit> .
pgc-1α has been found to be involved in de novo mitochondrial synthesis in various tissues including the liver and brain  <cit> .
medical data has shown that polymorphisms in pgc-1α have been commonly found in patients with early onset and severe pd  <cit> .
other members of this gene family, pgc-1β  <cit>  and prc   <cit>  have been shown to maintain some functional homology with pgc-1α and protein sequence comparison of these genes shows close similarity and conservation of active sites  <cit>  making it difficult to completely study the loss of function in human cells.
pgc-1α shares a functional pathway with two previously characterised pd genes; pink <dig> and parkin  <cit> .
parkin is a component of a multi-protein e <dig> ubiquitin ligase that leads to the ubiquitination and subsequent destruction of cellular proteins  <cit> .
mutations to the parkin gene result in the degeneration of dopaminergic  neurons, most likely by allowing the aggregation of multiple dysfunctional mitochondria that eventually lead to overall cell death  <cit> .
pink <dig>  is a serine/threonine-protein kinase that acts by recruiting parkin to damaged mitochondria  <cit> .
similarly to parkin, mutations in pink <dig> lead to degeneration and dysfunction of dopaminergic neurons  <cit> .
parkin and pink <dig> act together in concert along with mitofusin  <dig>  to remove any damaged or dysfunctional mitochondria that may be present while pgc-1α activity regulates the creation of new mitochondria to replace damaged or removed organelles  <cit> .
mutation to any of the components of this pathway can lead to impaired mitochondria, decreased cellular fitness and eventual cell death.
a single pgc family gene homologue, spargel , has been identified in drosophila melanogaster.
the srl protein has been characterized as a downstream component of the insulin signalling tor pathway and srl mutant flies have a “lean” phenotype typical of mutations that affect growth and proliferation and reduced mitochondrial fitness  <cit> .
although ubiquitous overexpression of srl has been found to negatively impact organism survival, tissue specific srl expression has been found to provide beneficial effects.
overexpression of srl has been shown to be sufficient to increase mitochondrial activity and mediate tissue specific lifespan extension in the digestive tract and intestine  <cit> .
altered expression of srl in the heart has been shown to increase capacity for exercise based endurance improvement while decreased srl in cardiac muscle decreases the loco-motor and endurance ability of flies  <cit> .
the lack of gene redundancy present in d. melanogaster makes it an ideal model system to determine the effects of reduced or increase levels of srl expression on whole organism and neuronal longevity, leading to a new model of pd for use in future therapeutic studies.
RESULTS
a multiple alignment of the srl protein with the three mammalian homologues; pgc-1α, pgc-1β and prc, provides evidence of evolutionarily conserved protein structure between the human and d. melanogaster forms of this gene .
these proteins differ in length from  <dig> amino acids in the case of prc to  <dig> amino acids for pgc-1α but functional domains remain consistent across all four.
each contains an n terminal proline rich domain, a bipartite nuclear localization signal, c terminal serine rich region and a highly conserved rna recognition motif as well as an arginine rich region in all except pgc-1β .
each of the mammalian proteins contain at least one leucine rich motif  known to interact with nuclear receptors  <cit>  that is not found in srl, however, an alternative leucine rich motif  is present which has been shown to also interact with nuclear receptors and serve the same function in d. melanogaster  <cit> .
the similarities between d. melanogaster and mammalian proteins indicate that srl is an ideal candidate to study pgc family activity while avoiding the functional redundancy found in other systems.fig.
<dig> the pgc mammalian family and the drosophila melanogaster protein srl share conserved protein domains.
a aligned sequences show the position of each domain in one d. melanogaster and three human pgc family protein sequences.
light upward diagonal indicates proline rich region, wide downward diagonal indicates serine rich region, dark vertical indicates rna recognition region, solid black box indicates nuclear localization sequence, black frame indicates arginine rich region, lxxll and fealll indicate leucine rich nuclear recognition motifs.
b a multiple alignment between pgc-1α, pgc-1β, prc and srl shows a high degree of sequence conservation within the rna recognition motif found at the carboxyl terminus of each protein.
domains were identified using scanprosite  <cit> , alignment was done using clustalw <dig>  <cit> .
protein sequences obtained from uniprot, accession numbers , ,  and .
elements of this figure were adapted from scarpulla et al.
<cit>
in order to assay the effect of altered srl activity in neurons, we induced expression of three constructs previously used by mukherjee and duttaroy  <cit> ; srl-rnai , srl-rnai  and a srl-ey  in the neuron rich d. melanogaster eye.
eyes develop two separate yet equally important tissues which can be assayed for neuronal loss during development; ommatidia and bristles.
at  <dig> and 29 °c  under the direction of gmr-gal <dig>  expression of both srl-rnai transgenes decreases the number of ommatidia and bristles present in the eye .
tissue specific expression of srl-ey results in a slight decrease in number of ommatidia and bristles at 25 °c and an increase in number of ommatidia and bristles at 29 °c.
similarly, uas-lacz under the control of gmr-gal <dig> shows a slight rough eye phenotype at 29 °c which is not produced by the expression of srl-ey.fig.
<dig> tissue specific srl expression in the drosophila melanogaster eye results in a reduction in both ommatidia and bristle number.
a scanning electron micrographs of d. melanogaster eyes taken at a horizontal field width of 500 µm.
genotypes are as follows:  gmr-gal4/uas-lacz 25 °c,  gmr-gal4/srl-rnai  <dig>  25 °c,  gmr-gal4/srl-rnai  <dig>  25 °c,  gmr–gal4/srl-ey  25 °c,  gmr-gal4/uas-lacz 29 °c,  gmr-gal4/srl-rnai  <dig>  29 °c,  gmr-gal4/srl-rnai  <dig>  29 °c,  gmr–gal4/srl-ey  29 °c.
images were taken with a fei mla  <dig>  b flies show a decrease in the mean number of ommatidia present when srl-rnai
<dig>  and srl-rnai
<dig>  are driven with the gmr-gal <dig> driver in both standard conditions  and at a higher temperature .
flies show a slight but not significant decrease in ommatidia number when srl-ey is expressed in a tissue specific manner  in both standard conditions  and at a higher temperature .
c flies show a strong decrease in bristle number in standard conditions  when srl-rnai
<dig> , srl-rnai  <dig>  and srl-ey  are expressed in d. melanogaster eyes.
at a higher temperature  srl-ey causes an increase in number of bristles formed compared to lacz controls, however, this is not statistically significant .
comparisons were measured using a one-way anova and significance was tested using a tukey post hoc test, n =  <dig>  *p <  <dig> , **p <  <dig> , ***p <  <dig>
to determine if the phenotype caused by altered srl expression found in the eye is conserved in all neurons we next looked at tissue specific expression of srl in the da neurons.
to assay the effect of altered srl activity in da neurons, we conditionally expressed a srl-rnai transgene  and a tissue specific srl-ey construct under the control of the ddc-gal <dig> driver.
under the control of ddc-gal <dig>  srl-rnai expression leads to a marked increase in median lifespan with a premature loss of climbing ability over time when compared to the uas-lacz controls .
tissue specific expression of srl-ey lead to both a decrease in lifespan and loco-motor climbing ability under the control of the ddc-gal <dig> driver.
this indicates that altered srl expression responds in a tissue specific nature, even within similar cell types such as neurons.
we obtained somewhat similar results when we carried out the experiment at 29 °c, indicating that the observed differences in phenotype between eye and dopaminergic neurons was tissue and not temperature specific.fig.
<dig> tissue specific altered srl expression in dopaminergic neurons can lead to a model of parkinson disease drosophila
melanogaster.
a expression of srl-rnai  driven by ddc-gal <dig> results in an increase in lifespan compared to uas-lacz controls.
dopaminergic srl-ey  expression resulted in a decrease in lifespan compared to uas-lacz controls.
longevity is shown as a percent survival  n ≥  <dig>  b expression of srl-rnai  and srl-ey  driven by ddc-gal <dig> cause a decrease in climbing ability over time.
dopaminergic specific expression of srl-ey causes a more severe decrease, however, srl-rnai expressing flies live longer and climb slightly worse than uas-lacz controls indicating a potential decrease in climbing activity compared to lifespan.
climbing ability was determined via nonlinear curve fit .
error bars indicate standard error of the mean, n =  <dig>  c expression of srl-rnai  driven by ddc-gal4
hl <dig> d at 29 °c results in an increase in lifespan while srl-ey  expression at 29 °c resulted in a decrease in lifespan compared to uas-lacz controls.
longevity is shown as a percent survival  n ≥ 150
discussion
pgc-1α has been identified as a modulator of mitochondrial biogenesis, energy metabolism  <cit> , insulin signalling  <cit>  and is believed to be linked to human ailments including alzheimer  <cit> , huntington  <cit>  and parkinson diseases  <cit> .
the study of human pgc-1α is complicated by the partial functional redundancy of the other pgc family members, pgc-1β and prc .
the single drosophila pgc family homologue, srl, has been linked to mitochondrial biogenesis and insulin signalling  <cit> .
we investigate srl as a potential key regulator of disease progression and seek to model this system for future analysis.
the d. melanogaster eye has been used to study potential genes involved in neurodegenerative disease due to the neuron rich nature of the developing tissues located there.
when two srl-rnai constructs are expressed in this tissue, there is a significant reduction in the number of ommatidia and bristles formed.
this effect is exacerbated when flies are raised at 29 °c, caused by an increase in the activity of the gal <dig> expression system.
alternatively, expression of a previously characterized srl-ey transgene seems to have little effect on ommatidial viability under normal physiological conditions .
at 29 °c the amount of ommatidial degeneration increases significantly across all genotypes.
interestingly, overexpression of srl-ey at 29 °c does not cause significant degeneration of ommatidia or bristles as found in the uas-lacz control at higher temperatures.
experiments by mukherjee and colleagues have shown that srl overexpression can rescue foxo mediated eye destruction, indicating a similar effect  <cit> .
it is possible that tissue specific expression of srl in the eye may be sufficient to prevent physiological stressors from causing aberrant cell formation under these conditions.
surprisingly, expression of srl-rnai in da neurons under the control of the ddc-gal <dig> driver caused an increase in the mean lifespan of flies.
despite the increased longevity of these flies, they lose their climbing ability slightly earlier than controls.
when considering the increase in lifespan this indicates that although they live longer, they may have severe loco-motor defects during the latter part of their life.
alternatively, da expression of the srl-ey transgene under the control of the ddc-gal <dig> driver showed a significant decrease in lifespan compared to uas-lacz controls.
the loco-motor and climbing ability of these flies was also decreased.
taken together, the premature mortality and decreased climbing ability displayed in flies expressing srl-ey in dopamine decarboxylase neurons appears to give a new, previously uncharacterized, model of pd in d. melanogaster.
altered srl expression has been found to cause various phenotypes in a tissue specific manner.
ubiquitous overexpression of srl has been shown to moderately reduce mean lifespan while overexpression in intestinal stem cells and cells of the digestive tract caused increased lifespan  <cit> .
similarly, increased srl expression in cardiac muscle has been linked to exercise based endurance improvement and cardiovascular performance  <cit> .
these findings lead us to believe that altered srl expression does not react the same way in the eye model of neurodegeneration as in da neurons.
although an increase in lifespan caused by the expression of srl-rnai was an unexpected result, we hypothesize that a strong decrease in srl expression causes an amount of mitochondrial stress sufficient to activate a protein stress response which has been shown to increase lifespan  <cit> .
the exact mechanism of this increase is a topic of much debate with the most popular hypothesis involving an activation of the unfolded protein response   <cit> .
however, it has recently been found that in caenorhabditis elegans many of the genes involved in the upr are non-essential for this increase in longevity, suggesting another mechanism for the increase in organismal longevity  <cit> .
alternatively, this increase in longevity may involve the concept of stress causing the formation of reactive oxygen species  at a low level which provoke cellular anti-oxidants, causing a stronger response to future ros exposure  <cit> .
the most commonly used term for this is mitochondrial hormesis   <cit> .
it is quite plausible that stress causes increased longevity through a combination of factors involving ideas from both of the aforementioned explanations.
CONCLUSIONS
the use of a model organism in identifying and characterizing the pathways of disease progression is a fundamental step in creating new and novel treatment options.
studying the consequences of altered srl expression in d. melanogaster allows us to study the complex mammalian pgc gene family in a system containing only a single homologue.
identifying the role of srl will lead to an understanding of the associations and pathways related to proper function of this gene and subsequently the consequences of improper gene function.
currently, there is no preventative treatment available for pd with limited options available to combat advanced symptoms.
identification of this new model of pd provides a framework for more advanced studies into complex gene interactions.
connecting cellular processes and characterizing genetic pathways of disease progression may eventually allow for the preventative treatment of genetic forms of pd and novel therapeutic options.

 parkinson disease is a common and progressive neurological disease that is estimated to afflict 1 % of all individuals over the age of 60 years worldwide. although the direct cause of pd is as of yet unknown, the clinical symptoms include resting tremor, bradykinesia, rigidity and postural instability. in addition to these classical symptoms recent discoveries have shown that effects may include cognitive impairment such as loss of memory and depression. these symptoms are caused by a decrease in the amount of dopamine available in the striatum area of the brain and in many cases characterized by an accumulation of harmful protein aggregates known as lewy bodies in the neurons of the substantia nigra pars compacta. the eventual dysfunction and breakdown of these neurons is responsible for the symptoms and pathology of pd. both genetic and environmental factors have been found to contribute to pd with many forms of the disease being attributed to a combination of the two. environmental factors include: chemical exposure, brain trauma, obesity, diabetes and age. alternatively, there have been a number of familial cases of pd identified which suggests a genetic link to certain forms of this disease. this link is especially strong in the case of early onset pd which shows a bias towards genetic causes, while environmental conditions seem to more often than not be linked to late onset pd. identifying and analysing the genes responsible for these inherited forms of pd may give rise to mechanisms of disease progression that are not presently understood. impaired neuronal activity has been shown to contribute to the lack of dopamine commonly associated with pd etiology. identifying the cause of this neuronal impairment may help lead to new, effective, strategies to combat pd. as important and necessary components of eukaryotic cells, mitochondria are integral components in the creation of adenosine tri-phosphate for use as chemical energy and are involved in other important pathways such as the control of cellular growth and death, cell signalling and differentiation. as the mitochondria are involved in such diverse and important cellular functions; it is not surprising that the breakdown or dysfunction of mitochondria may result in a number of disorders or diseases including but not limited to movement disorders such as pd. many of these disorders are caused by defective removal of damaged or non-functional mitochondria and a subsequent lack of de novo synthesis of new mitochondria to replace the aforementioned damaged organelles. thus, the study of genes that regulate and monitor the health of mitochondria is a reasonable next step for determining potential disease prevention strategies. the peroxisome proliferation activated co-receptor gamma family of genes have been linked to mitochondrial biogenesis. pgc-1α has been found to be involved in de novo mitochondrial synthesis in various tissues including the liver and brain. medical data has shown that polymorphisms in pgc-1α have been commonly found in patients with early onset and severe pd. other members of this gene family, pgc-1β and prc have been shown to maintain some functional homology with pgc-1α and protein sequence comparison of these genes shows close similarity and conservation of active sites making it difficult to completely study the loss of function in human cells. pgc-1α shares a functional pathway with two previously characterised pd genes; pink and parkin. parkin is a component of a multi-protein e ubiquitin ligase that leads to the ubiquitination and subsequent destruction of cellular proteins. mutations to the parkin gene result in the degeneration of dopaminergic neurons, most likely by allowing the aggregation of multiple dysfunctional mitochondria that eventually lead to overall cell death. pink is a serine/threonine-protein kinase that acts by recruiting parkin to damaged mitochondria. similarly to parkin, mutations in pink lead to degeneration and dysfunction of dopaminergic neurons. parkin and pink act together in concert along with mitofusin to remove any damaged or dysfunctional mitochondria that may be present while pgc-1α activity regulates the creation of new mitochondria to replace damaged or removed organelles. mutation to any of the components of this pathway can lead to impaired mitochondria, decreased cellular fitness and eventual cell death. a single pgc family gene homologue, spargel, has been identified in drosophila melanogaster. the srl protein has been characterized as a downstream component of the insulin signalling tor pathway and srl mutant flies have a “lean” phenotype typical of mutations that affect growth and proliferation and reduced mitochondrial fitness. although ubiquitous overexpression of srl has been found to negatively impact organism survival, tissue specific srl expression has been found to provide beneficial effects. overexpression of srl has been shown to be sufficient to increase mitochondrial activity and mediate tissue specific lifespan extension in the digestive tract and intestine. altered expression of srl in the heart has been shown to increase capacity for exercise based endurance improvement while decreased srl in cardiac muscle decreases the loco-motor and endurance ability of flies. the lack of gene redundancy present in d. melanogaster makes it an ideal model system to determine the effects of reduced or increase levels of srl expression on whole organism and neuronal longevity, leading to a new model of pd for use in future therapeutic studies.  a multiple alignment of the srl protein with the three mammalian homologues; pgc-1α, pgc-1β and prc, provides evidence of evolutionarily conserved protein structure between the human and d. melanogaster forms of this gene. these proteins differ in length from amino acids in the case of prc to amino acids for pgc-1α but functional domains remain consistent across all four. each contains an n terminal proline rich domain, a bipartite nuclear localization signal, c terminal serine rich region and a highly conserved rna recognition motif as well as an arginine rich region in all except pgc-1β. each of the mammalian proteins contain at least one leucine rich motif known to interact with nuclear receptors that is not found in srl, however, an alternative leucine rich motif is present which has been shown to also interact with nuclear receptors and serve the same function in d. melanogaster. the similarities between d. melanogaster and mammalian proteins indicate that srl is an ideal candidate to study pgc family activity while avoiding the functional redundancy found in other systems.fig.  the pgc mammalian family and the drosophila melanogaster protein srl share conserved protein domains. a aligned sequences show the position of each domain in one d. melanogaster and three human pgc family protein sequences. light upward diagonal indicates proline rich region, wide downward diagonal indicates serine rich region, dark vertical indicates rna recognition region, solid black box indicates nuclear localization sequence, black frame indicates arginine rich region, lxxll and fealll indicate leucine rich nuclear recognition motifs. b a multiple alignment between pgc-1α, pgc-1β, prc and srl shows a high degree of sequence conservation within the rna recognition motif found at the carboxyl terminus of each protein. domains were identified using scanprosite, alignment was done using clustalw. protein sequences obtained from uniprot, accession numbers,, and. elements of this figure were adapted from scarpulla et al. in order to assay the effect of altered srl activity in neurons, we induced expression of three constructs previously used by mukherjee and duttaroy; srl-rnai, srl-rnai and a srl-ey in the neuron rich d. melanogaster eye. eyes develop two separate yet equally important tissues which can be assayed for neuronal loss during development; ommatidia and bristles. at and 29 °c under the direction of gmr-gal expression of both srl-rnai transgenes decreases the number of ommatidia and bristles present in the eye. tissue specific expression of srl-ey results in a slight decrease in number of ommatidia and bristles at 25 °c and an increase in number of ommatidia and bristles at 29 °c. similarly, uas-lacz under the control of gmr-gal shows a slight rough eye phenotype at 29 °c which is not produced by the expression of srl-ey.fig.  tissue specific srl expression in the drosophila melanogaster eye results in a reduction in both ommatidia and bristle number. a scanning electron micrographs of d. melanogaster eyes taken at a horizontal field width of 500 µm. genotypes are as follows: gmr-gal4/uas-lacz 25 °c, gmr-gal4/srl-rnai 25 °c, gmr-gal4/srl-rnai 25 °c, gmr–gal4/srl-ey 25 °c, gmr-gal4/uas-lacz 29 °c, gmr-gal4/srl-rnai 29 °c, gmr-gal4/srl-rnai 29 °c, gmr–gal4/srl-ey 29 °c. images were taken with a fei mla b flies show a decrease in the mean number of ommatidia present when srl-rnai and srl-rnai are driven with the gmr-gal driver in both standard conditions and at a higher temperature. flies show a slight but not significant decrease in ommatidia number when srl-ey is expressed in a tissue specific manner in both standard conditions and at a higher temperature. c flies show a strong decrease in bristle number in standard conditions when srl-rnai, srl-rnai and srl-ey are expressed in d. melanogaster eyes. at a higher temperature srl-ey causes an increase in number of bristles formed compared to lacz controls, however, this is not statistically significant. comparisons were measured using a one-way anova and significance was tested using a tukey post hoc test, n =  *p < , **p < , ***p <  to determine if the phenotype caused by altered srl expression found in the eye is conserved in all neurons we next looked at tissue specific expression of srl in the da neurons. to assay the effect of altered srl activity in da neurons, we conditionally expressed a srl-rnai transgene and a tissue specific srl-ey construct under the control of the ddc-gal driver. under the control of ddc-gal srl-rnai expression leads to a marked increase in median lifespan with a premature loss of climbing ability over time when compared to the uas-lacz controls. tissue specific expression of srl-ey lead to both a decrease in lifespan and loco-motor climbing ability under the control of the ddc-gal driver. this indicates that altered srl expression responds in a tissue specific nature, even within similar cell types such as neurons. we obtained somewhat similar results when we carried out the experiment at 29 °c, indicating that the observed differences in phenotype between eye and dopaminergic neurons was tissue and not temperature specific.fig.  tissue specific altered srl expression in dopaminergic neurons can lead to a model of parkinson disease drosophila melanogaster. a expression of srl-rnai driven by ddc-gal results in an increase in lifespan compared to uas-lacz controls. dopaminergic srl-ey expression resulted in a decrease in lifespan compared to uas-lacz controls. longevity is shown as a percent survival n ≥  b expression of srl-rnai and srl-ey driven by ddc-gal cause a decrease in climbing ability over time. dopaminergic specific expression of srl-ey causes a more severe decrease, however, srl-rnai expressing flies live longer and climb slightly worse than uas-lacz controls indicating a potential decrease in climbing activity compared to lifespan. climbing ability was determined via nonlinear curve fit. error bars indicate standard error of the mean, n =  c expression of srl-rnai driven by ddc-gal4 hl d at 29 °c results in an increase in lifespan while srl-ey expression at 29 °c resulted in a decrease in lifespan compared to uas-lacz controls. longevity is shown as a percent survival n ≥ 150 discussion pgc-1α has been identified as a modulator of mitochondrial biogenesis, energy metabolism, insulin signalling and is believed to be linked to human ailments including alzheimer, huntington and parkinson diseases. the study of human pgc-1α is complicated by the partial functional redundancy of the other pgc family members, pgc-1β and prc. the single drosophila pgc family homologue, srl, has been linked to mitochondrial biogenesis and insulin signalling. we investigate srl as a potential key regulator of disease progression and seek to model this system for future analysis. the d. melanogaster eye has been used to study potential genes involved in neurodegenerative disease due to the neuron rich nature of the developing tissues located there. when two srl-rnai constructs are expressed in this tissue, there is a significant reduction in the number of ommatidia and bristles formed. this effect is exacerbated when flies are raised at 29 °c, caused by an increase in the activity of the gal expression system. alternatively, expression of a previously characterized srl-ey transgene seems to have little effect on ommatidial viability under normal physiological conditions. at 29 °c the amount of ommatidial degeneration increases significantly across all genotypes. interestingly, overexpression of srl-ey at 29 °c does not cause significant degeneration of ommatidia or bristles as found in the uas-lacz control at higher temperatures. experiments by mukherjee and colleagues have shown that srl overexpression can rescue foxo mediated eye destruction, indicating a similar effect. it is possible that tissue specific expression of srl in the eye may be sufficient to prevent physiological stressors from causing aberrant cell formation under these conditions. surprisingly, expression of srl-rnai in da neurons under the control of the ddc-gal driver caused an increase in the mean lifespan of flies. despite the increased longevity of these flies, they lose their climbing ability slightly earlier than controls. when considering the increase in lifespan this indicates that although they live longer, they may have severe loco-motor defects during the latter part of their life. alternatively, da expression of the srl-ey transgene under the control of the ddc-gal driver showed a significant decrease in lifespan compared to uas-lacz controls. the loco-motor and climbing ability of these flies was also decreased. taken together, the premature mortality and decreased climbing ability displayed in flies expressing srl-ey in dopamine decarboxylase neurons appears to give a new, previously uncharacterized, model of pd in d. melanogaster. altered srl expression has been found to cause various phenotypes in a tissue specific manner. ubiquitous overexpression of srl has been shown to moderately reduce mean lifespan while overexpression in intestinal stem cells and cells of the digestive tract caused increased lifespan. similarly, increased srl expression in cardiac muscle has been linked to exercise based endurance improvement and cardiovascular performance. these findings lead us to believe that altered srl expression does not react the same way in the eye model of neurodegeneration as in da neurons. although an increase in lifespan caused by the expression of srl-rnai was an unexpected result, we hypothesize that a strong decrease in srl expression causes an amount of mitochondrial stress sufficient to activate a protein stress response which has been shown to increase lifespan. the exact mechanism of this increase is a topic of much debate with the most popular hypothesis involving an activation of the unfolded protein response. however, it has recently been found that in caenorhabditis elegans many of the genes involved in the upr are non-essential for this increase in longevity, suggesting another mechanism for the increase in organismal longevity. alternatively, this increase in longevity may involve the concept of stress causing the formation of reactive oxygen species at a low level which provoke cellular anti-oxidants, causing a stronger response to future ros exposure. the most commonly used term for this is mitochondrial hormesis. it is quite plausible that stress causes increased longevity through a combination of factors involving ideas from both of the aforementioned explanations.  the use of a model organism in identifying and characterizing the pathways of disease progression is a fundamental step in creating new and novel treatment options. studying the consequences of altered srl expression in d. melanogaster allows us to study the complex mammalian pgc gene family in a system containing only a single homologue. identifying the role of srl will lead to an understanding of the associations and pathways related to proper function of this gene and subsequently the consequences of improper gene function. currently, there is no preventative treatment available for pd with limited options available to combat advanced symptoms. identification of this new model of pd provides a framework for more advanced studies into complex gene interactions. connecting cellular processes and characterizing genetic pathways of disease progression may eventually allow for the preventative treatment of genetic forms of pd and novel therapeutic options. 
directed expression of srl-rnai in the d. melanogaster eye causes abnormal ommatidia and bristle formation while eye specific expression of srl-ey does not produce the minor rough eye phenotype associated with high temperature gmr-gal expression. ddc-gal mediated tissue specific expression of srl transgene constructs in d. melanogaster da neurons causes altered lifespan and climbing ability. the reduced lifespan and climbing ability associated with a tissue specific expression of srl in da neurons provides a new model of pd in d. melanogaster which may be used to identify novel therapeutic approaches to human disease treatment and prevention. parkinson disease is a progressive neurodegenerative disorder presenting with symptoms of resting tremor, bradykinesia, rigidity, postural instability and additional severe cognitive impairment over time. spargelpgc-1αneurodegenerationparkinson diseasedrosophila melanogasterissue-copyright-statement© the author 2015 expression of a srl-rnai causes an increase in mean lifespan but a decrease in overall loco-motor ability while induced expression of srl-ey causes a severe decrease in mean lifespan and a decrease in loco-motor ability. the sole drosophila melanogaster homologue of this gene family, spargel, has been shown to function in similar pathways of mitochondrial upkeep and biogenesis.
 parkinson disease is a progressive neurodegenerative disorder presenting with symptoms of resting tremor, bradykinesia, rigidity, postural instability and additional severe cognitive impairment over time. these symptoms arise from a decrease of available dopamine in the striatum of the brain resulting from the breakdown and death of dopaminergic neurons. a process implicated in the destruction of these neurons is mitochondrial breakdown and impairment. upkeep and repair of mitochondria involves a number of complex and key components including pink parkin, and the pgc family of genes. pgc-1α has been characterized as a regulator of mitochondria biogenesis, insulin receptor signalling and energy metabolism, mutation of this gene has been linked to early onset forms of pd. the mammalian pgc family consists of three partially redundant genes making the study of full or partial loss of function difficult. the sole drosophila melanogaster homologue of this gene family, spargel, has been shown to function in similar pathways of mitochondrial upkeep and biogenesis.  directed expression of srl-rnai in the d. melanogaster eye causes abnormal ommatidia and bristle formation while eye specific expression of srl-ey does not produce the minor rough eye phenotype associated with high temperature gmr-gal expression. ddc-gal mediated tissue specific expression of srl transgene constructs in d. melanogaster da neurons causes altered lifespan and climbing ability. expression of a srl-rnai causes an increase in mean lifespan but a decrease in overall loco-motor ability while induced expression of srl-ey causes a severe decrease in mean lifespan and a decrease in loco-motor ability.  the reduced lifespan and climbing ability associated with a tissue specific expression of srl in da neurons provides a new model of pd in d. melanogaster which may be used to identify novel therapeutic approaches to human disease treatment and prevention. keywords spargelpgc-1αneurodegenerationparkinson diseasedrosophila melanogasterissue-copyright-statement© the author 2015 