BACKGROUND
salmonella is one of the major zoonotic foodborne pathogens worldwide.
it can cause a variety of clinical manifestations from mild gastroenteritis to bacteremia and typhoid fever.
the global burden of nontyphoidal salmonella gastroenteritis has been estimated to be  <dig>  million cases of gastroenteritis each year, with  <dig>  <dig> deaths  <cit> .
in africa, non-typhoidal salmonella has consistently been reported as a leading cause of bacteremia among immuno-compromised people, infants and newborns  <cit> .
however, the sources and transmission routes of salmonella in developing countries are poorly understood due to the lack of coordinated national epidemiological surveillance systems  <cit> .
in general, the primary sources of salmonellosis are considered to be food-producing animals such as cattle, poultry and swine  <cit> .
the pathogens are mainly disseminated by trade in animals and uncooked animal food products  <cit> .
the process of removing the gastrointestinal tract during slaughtering of food animals is regarded as one of the most important sources of carcass and organ contamination with salmonella at abattoirs  <cit> .
also asymptomatic pet animals are a potential source of infection, especially species with high fecal carriage rates of salmonella <cit> .
african pygmy hedgehogs kept as pets have previously been associated with cases of human salmonellosis  <cit> .
the development and the accumulation of resistance to antimicrobials in foodborne pathogens are a major problem for public health.
multi-resistant salmonella may acquire their resistance genes from microbiota of production animals before being transmitted to humans through food chain  <cit> .
due to the lacking surveillance programs in burkina faso, as in the most of africa, information on the prevalence of salmonella and other enteropathogens in food stuffs is limited.
however, our previous study on the prevalence of enteric bacteria on retail meats sold at the markets in ouagadougou, burkina faso, revealed that 37% of the chicken, 13% of the beef intestines, and 7% of the mutton samples were contaminated by salmonella <cit> .
the most common serotypes detected were s. derby and s. tilene.
in a following broader study on chicken carcasses in burkina faso, up to 57% of the carcasses were found to be contaminated by salmonella, s. derby again being the most common serotype  <cit> .
in order to better understand the origin of the pathogens, in the current study, we sampled the feces of the common food animals during slaughter.
since previously s. tilene has mainly been recovered from african pigmy hedgehogs kept as pets in north america or europe  <cit> , we included hedgehogs, which are common on the grassy pastures in burkina faso and also consumed as food, in our study.
the specific aims of our study were: first, to estimate the prevalence of salmonella in the feces of slaughtered cattle, poultry and swine, as well as in the feces of hedgehogs in burkina faso; second, to identify the serotype of salmonella isolates; third, to determine the sensitivity of the isolates to the antimicrobial agents; and finally, to assess the genetic relatedness of the isolates from the feces of the animals and from the local children using pulsed-field gel electrophoresis .
RESULTS
salmonella prevalence and the serotypes
salmonella was isolated from  <dig>  of the total of  <dig> feces samples from apparently healthy animals.
isolates were obtained from  <dig>  of the cattle feces,  <dig>  of the chicken feces,  <dig>  of the swine feces and  <dig>  of the hedgehog feces .
of the  <dig> isolates,  <dig> belonged to s. enterica ssp.
enterica and one was s. enterica ssp.
salamae.
<dig> of the s. enterica ssp.
enterica isolates could be serotyped in detail, while for  <dig> isolates only the salmonella group could be assigned.
<dig> different serotypes were found from the cattle,  <dig> from the chicken,  <dig> from the swine and  <dig> from the hedgehog feces.
the predominant serotypes were s. drac and s. muenster in the cattle, s. derby and s. chester in the poultry and s. muenster in both the swine and hedgehog feces.
the 3 s. typhimurium isolates from the cattle all belonged to variant copenhagen.
phage typing divided the s. typhimurium isolates further into three definite phage types: dts  <dig>   <dig> and  <dig> .
in addition,  <dig> strains were rdnc .
salmonella enterica
serotypes isolated from cattle, poultry, swine and hedgehog feces and their antimicrobial resistance patterns
salmonella
resistant
a
a
afor example, entry 7pstr-tet, means that  <dig> isolates from poultry feces were resistant/intermediate to streptomycin and tetracycline.
abbreviations: c, cattle feces; p, poultry feces; s, swine feces; h, hedgehog feces, amp, ampicillin; chl, chloramphenicol; str, streptomycin; sul, sulphonamides; tmp, trimethoprim; tet, tetracycline; nal, nalidixic acid; cip, ciprofloxacin; ftx, cefotaxime; mec, mecillinam.
antimicrobial resistance
on the whole,  <dig>  of the  <dig> salmonella isolates were resistant to one or more antimicrobials tested:  <dig> of these were from the cattle,  <dig> from the poultry and  <dig> from the hedgehog feces .
the salmonella isolates from the swine feces were susceptible to the tested antimicrobials.
six isolates were multiresistant: 4 s. typhimurium isolates from the poultry feces , 1 s. hato isolate from the poultry feces  and 1 s. urbana isolate from the cattle feces .
out of the  <dig> isolates,  <dig>  showed decreased sensitivity  to one or more antimicrobial, especially to streptomycin, tetracycline and sulphonamides .
two isolates  had decreased sensitivity to ciprofloxacin and one  to cefotaxime.
the mic values for the nalidixic acid resistant isolates were  <dig>  μg/ml  and  <dig>  μg/ml .
genetic relatedness by pfge
to determine the genotypic relatedness of the salmonella isolates recovered from the cattle, poultry, swine and hedgehog feces and to compare them to human isolates from burkina faso  <cit> , a total of  <dig> isolates were subjected to pfge analysis with xbai and blni restriction enzymes .
genetic relatedness of the isolates belonging to the same serotype ranged from approximately 70% to 100%.
s. typhimurium isolates from the poultry and human feces clustered closely together.
s. muenster isolates obtained from the cattle and swine feces were different, but both clustered closely together with some hedgehog isolates .
two s. typhimurium var.
copenhagen isolates from the cattle feces clustered together with the s. typhimurium isolates when xbai was used, whereas all three were distinct from s. typhimurium when blni was used.
s. albany isolates from the cattle and poultry feces clustered separately using both enzymes.
discussion
we detected high prevalence of salmonella enterica ssp.
enterica in the feces of the production animals slaughtered for human consumption in burkina faso.
salmonella was especially common in the poultry  and cattle  feces samples.
the levels of salmonella in poultry can vary depending on the country, the nature of the production system and the specific control measures in place.
in some eu countries chicken flocks are virtually free from salmonella whereas in the us a contamination rate up to 60% was detected  <cit> .
in japan, salmonella was isolated from 36% of the broiler fecal samples  <cit> .
in gambia, the detected rate of salmonella in chicken feces was higher, 67%  <cit> , than what we detected from the chicken feces.
in comparison, only 11% of chicken reared at intensive poultry farms in nigeria were found to be infected  <cit> .
the levels of salmonella rates reported in beef are usually lower than in chicken.
salmonella carriage was reported to be  <dig> % in cattle in great britain  <cit>  and  <dig> % in japan  <cit> .
in ethiopia, 4% of the feces of slaughtered cattle were contaminated by salmonella <cit> .
the high rate of salmonella detected in our study might be explained partly by the method used for strain isolation and partly by the animal husbandry practices.
in burkina faso, cows and sheep mostly roam freely at pasture in the bush.
the wild animals, such as hedgehogs, living in such places can contaminate grass with their excreta, which, as shown in our study, can have high carriage rate of salmonella.
we found 16% of the swine feces samples to be contaminated by salmonella.
salmonella contamination rates for pigs reported in literature vary from 9% to 23% in europe  <cit> , to 3% of porcine fecal samples in japan  <cit>  and 8% in kenya  <cit> .
in accordance to the high rates of salmonella detected in the feces samples, our previous studies on the prevalence of salmonella in retail meats and beef intestines in burkina faso also revealed high numbers of salmonella, especially in chicken   <cit> .
several of the serotypes isolated in this study, including s. typhimurium, s. muenster, s. derby, s. virchow, s. hato, s. bredeney, s. stanley and s. anatum, have frequently been implicated in outbreaks or sporadic cases of human illness  <cit> .
in africa, as elsewhere in the world, s. enteritidis and s. typhimurium are the most common causes of human salmonellosis  <cit> .
interestingly, s. enteritidis was not recovered from the animal feces in our study and not from the human isolates in burkina faso either  <cit> .
the main serotypes found in both animal and human feces samples from burkina faso included s. typhimurium  and s. muenster .
s. derby was the most common serotype we detected in the chicken feces, as it was in the chicken carcasses  <cit> .
world-wide, a wide range of salmonella serotypes have the ability to colonize poultry: s. typhimurium, s. enteritidis, s. hadar, s. virchow, s. infantis and, recently, s. paratyphi b var.
java have all been frequently isolated from poultry in several countries  <cit> , none of which were among the most common serotypes in poultry in burkina faso.
elsewhere in africa, s. enteritidis was the most common serotype detected in chicken feces in zimbabwe  <cit>  and s. typhimurium in algeria  <cit> .
notably, we isolated one s. typhi strain from the chicken feces, as we did previously from a chicken carcass  <cit> .
the s. typhimurium isolates from chicken feces in burkina faso were multi-resistant to the commonly available antimicrobials ampicillin, chloramphenicol, streptomycin, sulfonamides and trimethoprim.
this is a typical pattern found in the salmonella strains with a sub-saharan distinct genotype causing epidemic invasive disease  <cit> .
bacteremia caused by multi-resistant s. typhimurium strains is a serious public health problem in africa and they are significantly associated with increased mortality  <cit> .
such s. typhimurium isolates have been reported from e.g.
zaire  <cit> , kenya  <cit> , malawi  <cit>  and central africa  <cit> .
although antimicrobial use for animals is under veterinary prescription control in burkina faso, farmers still use unprescribed antimicrobials as growth promoters or treatment for cattle, poultry and swine.
this practice leads into a possibility that bacterial resistance developing in the food animals transfers to the human population thus posing a risk for public health by spreading of the resistance  <cit> .
it would be essential to study the genotype of our s. typhimurium isolates from poultry further in order to know if the invasive genotype also occurs in animals as the environmental reservoirs and host ranges of invasive salmonella strains in africa are still unknown  <cit> .
our s. typhimurium isolates from chicken and humans had the same phage type dt  <dig>  this phage type was in kenya among the most common phage type from adult patients  <cit> .
in developed countries, a phage type dt  <dig> has often been associated with outbreaks of multiresistant s. typhimurium infection in both man and animals  <cit> .
only two isolates in our study was resistant to the newer antimicrobials; s. muenster from the poultry feces was resistant to nalidixic acid, as was s. urbana from the cattle feces, furthermore, its sensitivity to ciprofloxacin and cefotaxime was decreased.
pfge provides valuable phylogenetic-relationship inference for salmonella at serotype and strain level  <cit> .
our cluster analysis revealed close genetic relationship between some human and animal strains belonging to the same serotypes.
notable similarity of the chicken and human isolates indicates that chicken may be a major source of salmonella transmission to humans.
also in senegal, a study detected a high degree of similarity among s. hadar, s. brancaster and s. enteritidis from poultry meat and humans by using pfge  <cit> .
besides through food, direct transmission from chicken to humans could easily happen in burkina faso, since chickens roam free scattering their feces anywhere in the house yards.
although, in these surroundings it is also possible that it is rather chicken which get transiently infected with the typical human salmonella strains.
however, the study conducted recently on isolates from infected children and their households in the gambia did not support the hypothesis that humans and animals living in close contact in the same household carry genotypically similar salmonella serotypes  <cit> .
we found out that the prevalence of salmonella in hedgehog feces was particularly high .
in burkina faso, hedgehogs live in a variety of habitats where they dig their burrows, spend most of the daylight hours asleep, and emerge at night to forage.
hedgehogs can serve as reservoirs of salmonella in many ways.
during the night, villagers go to catch them as a meat source for the next day.
during the rainy season, feces of animals including hedgehogs pollute the water sources such as rivers and wells.
at the countryside many people are dependent on these sources for their potable water.
in developed countries, people having exotic hedgehogs as pets have fallen sick with salmonellosis  <cit> .
in these cases, the commonly detected salmonella serotype has been s. tilene  <cit> .
since we found several s. tilene strains in our cattle and chicken meat samples during our previous study  <cit> , we wanted to investigate a possible link between the salmonella carriage of the production animals and hedgehogs, which share the same pastures for foraging.
indeed, we found hedgehogs in burkina faso to carry many salmonella serotypes common also in the production animals, but no s. tilene was detected, not in feces of the studied hedgehogs or of the other animals.
s. muenster isolates were obtained from the feces of all the studied animal species and humans and their genetic relatedness in pfge analysis was  <dig> to 95%.
thus, it is possible that the same strains of s. muenster are able to infect many different hosts.
hedgehog feces might infect both cattle and swine foraging freely, since salmonella can persist in the environment for several months to more than a year  <cit> .
the production animals and the hedgehogs might all be able to transfer salmonella further to the humans.
we have previously shown the production animals to be potential carriers of virulent escherichia coli to humans as well  <cit> .
there is no previous information on the frequency of wild animals carrying enteropathogenic bacteria in burkina faso, apart from the salmonella carriage of hedgehogs reported here.
CONCLUSIONS
our study revealed that both production and some wild animals commonly carry salmonella in burkina faso.
some of the isolated salmonella strains were genetically related to the human salmonella strains and resistant to the common antimicrobials.
as the humans and animals often live in close vicinity in africa and the hygiene control of the meat retail chain is defective, high carriage rates of salmonella and other potential pathogens of asymptomatic production animals can pose a major public health problem in burkina faso.
therefore, systematic surveillance of the infection sources and routes of the bacterial pathogens especially in the food production chain is needed to target the control actions to the critical points in the spread of the pathogens to the consumers.

 salmonella is one of the major zoonotic foodborne pathogens worldwide. it can cause a variety of clinical manifestations from mild gastroenteritis to bacteremia and typhoid fever. the global burden of nontyphoidal salmonella gastroenteritis has been estimated to be million cases of gastroenteritis each year, with deaths. in africa, non-typhoidal salmonella has consistently been reported as a leading cause of bacteremia among immuno-compromised people, infants and newborns. however, the sources and transmission routes of salmonella in developing countries are poorly understood due to the lack of coordinated national epidemiological surveillance systems. in general, the primary sources of salmonellosis are considered to be food-producing animals such as cattle, poultry and swine. the pathogens are mainly disseminated by trade in animals and uncooked animal food products. the process of removing the gastrointestinal tract during slaughtering of food animals is regarded as one of the most important sources of carcass and organ contamination with salmonella at abattoirs. also asymptomatic pet animals are a potential source of infection, especially species with high fecal carriage rates of salmonella. african pygmy hedgehogs kept as pets have previously been associated with cases of human salmonellosis. the development and the accumulation of resistance to antimicrobials in foodborne pathogens are a major problem for public health. multi-resistant salmonella may acquire their resistance genes from microbiota of production animals before being transmitted to humans through food chain. due to the lacking surveillance programs in burkina faso, as in the most of africa, information on the prevalence of salmonella and other enteropathogens in food stuffs is limited. however, our previous study on the prevalence of enteric bacteria on retail meats sold at the markets in ouagadougou, burkina faso, revealed that 37% of the chicken, 13% of the beef intestines, and 7% of the mutton samples were contaminated by salmonella. the most common serotypes detected were s. derby and s. tilene. in a following broader study on chicken carcasses in burkina faso, up to 57% of the carcasses were found to be contaminated by salmonella, s. derby again being the most common serotype. in order to better understand the origin of the pathogens, in the current study, we sampled the feces of the common food animals during slaughter. since previously s. tilene has mainly been recovered from african pigmy hedgehogs kept as pets in north america or europe, we included hedgehogs, which are common on the grassy pastures in burkina faso and also consumed as food, in our study. the specific aims of our study were: first, to estimate the prevalence of salmonella in the feces of slaughtered cattle, poultry and swine, as well as in the feces of hedgehogs in burkina faso; second, to identify the serotype of salmonella isolates; third, to determine the sensitivity of the isolates to the antimicrobial agents; and finally, to assess the genetic relatedness of the isolates from the feces of the animals and from the local children using pulsed-field gel electrophoresis.  salmonella prevalence and the serotypes salmonella was isolated from of the total of feces samples from apparently healthy animals. isolates were obtained from of the cattle feces, of the chicken feces, of the swine feces and of the hedgehog feces. of the isolates, belonged to s. enterica ssp. enterica and one was s. enterica ssp. salamae. of the s. enterica ssp. enterica isolates could be serotyped in detail, while for isolates only the salmonella group could be assigned. different serotypes were found from the cattle, from the chicken, from the swine and from the hedgehog feces. the predominant serotypes were s. drac and s. muenster in the cattle, s. derby and s. chester in the poultry and s. muenster in both the swine and hedgehog feces. the 3 s. typhimurium isolates from the cattle all belonged to variant copenhagen. phage typing divided the s. typhimurium isolates further into three definite phage types: dts and. in addition, strains were rdnc. salmonella enterica serotypes isolated from cattle, poultry, swine and hedgehog feces and their antimicrobial resistance patterns salmonella resistant a a afor example, entry 7pstr-tet, means that isolates from poultry feces were resistant/intermediate to streptomycin and tetracycline. abbreviations: c, cattle feces; p, poultry feces; s, swine feces; h, hedgehog feces, amp, ampicillin; chl, chloramphenicol; str, streptomycin; sul, sulphonamides; tmp, trimethoprim; tet, tetracycline; nal, nalidixic acid; cip, ciprofloxacin; ftx, cefotaxime; mec, mecillinam. antimicrobial resistance on the whole, of the salmonella isolates were resistant to one or more antimicrobials tested: of these were from the cattle, from the poultry and from the hedgehog feces. the salmonella isolates from the swine feces were susceptible to the tested antimicrobials. six isolates were multiresistant: 4 s. typhimurium isolates from the poultry feces, 1 s. hato isolate from the poultry feces and 1 s. urbana isolate from the cattle feces. out of the isolates, showed decreased sensitivity to one or more antimicrobial, especially to streptomycin, tetracycline and sulphonamides. two isolates had decreased sensitivity to ciprofloxacin and one to cefotaxime. the mic values for the nalidixic acid resistant isolates were  μg/ml and  μg/ml. genetic relatedness by pfge to determine the genotypic relatedness of the salmonella isolates recovered from the cattle, poultry, swine and hedgehog feces and to compare them to human isolates from burkina faso, a total of isolates were subjected to pfge analysis with xbai and blni restriction enzymes. genetic relatedness of the isolates belonging to the same serotype ranged from approximately 70% to 100%. s. typhimurium isolates from the poultry and human feces clustered closely together. s. muenster isolates obtained from the cattle and swine feces were different, but both clustered closely together with some hedgehog isolates. two s. typhimurium var. copenhagen isolates from the cattle feces clustered together with the s. typhimurium isolates when xbai was used, whereas all three were distinct from s. typhimurium when blni was used. s. albany isolates from the cattle and poultry feces clustered separately using both enzymes. discussion we detected high prevalence of salmonella enterica ssp. enterica in the feces of the production animals slaughtered for human consumption in burkina faso. salmonella was especially common in the poultry and cattle feces samples. the levels of salmonella in poultry can vary depending on the country, the nature of the production system and the specific control measures in place. in some eu countries chicken flocks are virtually free from salmonella whereas in the us a contamination rate up to 60% was detected. in japan, salmonella was isolated from 36% of the broiler fecal samples. in gambia, the detected rate of salmonella in chicken feces was higher, 67%, than what we detected from the chicken feces. in comparison, only 11% of chicken reared at intensive poultry farms in nigeria were found to be infected. the levels of salmonella rates reported in beef are usually lower than in chicken. salmonella carriage was reported to be % in cattle in great britain and % in japan. in ethiopia, 4% of the feces of slaughtered cattle were contaminated by salmonella. the high rate of salmonella detected in our study might be explained partly by the method used for strain isolation and partly by the animal husbandry practices. in burkina faso, cows and sheep mostly roam freely at pasture in the bush. the wild animals, such as hedgehogs, living in such places can contaminate grass with their excreta, which, as shown in our study, can have high carriage rate of salmonella. we found 16% of the swine feces samples to be contaminated by salmonella. salmonella contamination rates for pigs reported in literature vary from 9% to 23% in europe, to 3% of porcine fecal samples in japan and 8% in kenya. in accordance to the high rates of salmonella detected in the feces samples, our previous studies on the prevalence of salmonella in retail meats and beef intestines in burkina faso also revealed high numbers of salmonella, especially in chicken. several of the serotypes isolated in this study, including s. typhimurium, s. muenster, s. derby, s. virchow, s. hato, s. bredeney, s. stanley and s. anatum, have frequently been implicated in outbreaks or sporadic cases of human illness. in africa, as elsewhere in the world, s. enteritidis and s. typhimurium are the most common causes of human salmonellosis. interestingly, s. enteritidis was not recovered from the animal feces in our study and not from the human isolates in burkina faso either. the main serotypes found in both animal and human feces samples from burkina faso included s. typhimurium and s. muenster. s. derby was the most common serotype we detected in the chicken feces, as it was in the chicken carcasses. world-wide, a wide range of salmonella serotypes have the ability to colonize poultry: s. typhimurium, s. enteritidis, s. hadar, s. virchow, s. infantis and, recently, s. paratyphi b var. java have all been frequently isolated from poultry in several countries, none of which were among the most common serotypes in poultry in burkina faso. elsewhere in africa, s. enteritidis was the most common serotype detected in chicken feces in zimbabwe and s. typhimurium in algeria. notably, we isolated one s. typhi strain from the chicken feces, as we did previously from a chicken carcass. the s. typhimurium isolates from chicken feces in burkina faso were multi-resistant to the commonly available antimicrobials ampicillin, chloramphenicol, streptomycin, sulfonamides and trimethoprim. this is a typical pattern found in the salmonella strains with a sub-saharan distinct genotype causing epidemic invasive disease. bacteremia caused by multi-resistant s. typhimurium strains is a serious public health problem in africa and they are significantly associated with increased mortality. such s. typhimurium isolates have been reported from e.g. zaire, kenya, malawi and central africa. although antimicrobial use for animals is under veterinary prescription control in burkina faso, farmers still use unprescribed antimicrobials as growth promoters or treatment for cattle, poultry and swine. this practice leads into a possibility that bacterial resistance developing in the food animals transfers to the human population thus posing a risk for public health by spreading of the resistance. it would be essential to study the genotype of our s. typhimurium isolates from poultry further in order to know if the invasive genotype also occurs in animals as the environmental reservoirs and host ranges of invasive salmonella strains in africa are still unknown. our s. typhimurium isolates from chicken and humans had the same phage type dt this phage type was in kenya among the most common phage type from adult patients. in developed countries, a phage type dt has often been associated with outbreaks of multiresistant s. typhimurium infection in both man and animals. only two isolates in our study was resistant to the newer antimicrobials; s. muenster from the poultry feces was resistant to nalidixic acid, as was s. urbana from the cattle feces, furthermore, its sensitivity to ciprofloxacin and cefotaxime was decreased. pfge provides valuable phylogenetic-relationship inference for salmonella at serotype and strain level. our cluster analysis revealed close genetic relationship between some human and animal strains belonging to the same serotypes. notable similarity of the chicken and human isolates indicates that chicken may be a major source of salmonella transmission to humans. also in senegal, a study detected a high degree of similarity among s. hadar, s. brancaster and s. enteritidis from poultry meat and humans by using pfge. besides through food, direct transmission from chicken to humans could easily happen in burkina faso, since chickens roam free scattering their feces anywhere in the house yards. although, in these surroundings it is also possible that it is rather chicken which get transiently infected with the typical human salmonella strains. however, the study conducted recently on isolates from infected children and their households in the gambia did not support the hypothesis that humans and animals living in close contact in the same household carry genotypically similar salmonella serotypes. we found out that the prevalence of salmonella in hedgehog feces was particularly high. in burkina faso, hedgehogs live in a variety of habitats where they dig their burrows, spend most of the daylight hours asleep, and emerge at night to forage. hedgehogs can serve as reservoirs of salmonella in many ways. during the night, villagers go to catch them as a meat source for the next day. during the rainy season, feces of animals including hedgehogs pollute the water sources such as rivers and wells. at the countryside many people are dependent on these sources for their potable water. in developed countries, people having exotic hedgehogs as pets have fallen sick with salmonellosis. in these cases, the commonly detected salmonella serotype has been s. tilene. since we found several s. tilene strains in our cattle and chicken meat samples during our previous study, we wanted to investigate a possible link between the salmonella carriage of the production animals and hedgehogs, which share the same pastures for foraging. indeed, we found hedgehogs in burkina faso to carry many salmonella serotypes common also in the production animals, but no s. tilene was detected, not in feces of the studied hedgehogs or of the other animals. s. muenster isolates were obtained from the feces of all the studied animal species and humans and their genetic relatedness in pfge analysis was to 95%. thus, it is possible that the same strains of s. muenster are able to infect many different hosts. hedgehog feces might infect both cattle and swine foraging freely, since salmonella can persist in the environment for several months to more than a year. the production animals and the hedgehogs might all be able to transfer salmonella further to the humans. we have previously shown the production animals to be potential carriers of virulent escherichia coli to humans as well. there is no previous information on the frequency of wild animals carrying enteropathogenic bacteria in burkina faso, apart from the salmonella carriage of hedgehogs reported here.  our study revealed that both production and some wild animals commonly carry salmonella in burkina faso. some of the isolated salmonella strains were genetically related to the human salmonella strains and resistant to the common antimicrobials. as the humans and animals often live in close vicinity in africa and the hygiene control of the meat retail chain is defective, high carriage rates of salmonella and other potential pathogens of asymptomatic production animals can pose a major public health problem in burkina faso. therefore, systematic surveillance of the infection sources and routes of the bacterial pathogens especially in the food production chain is needed to target the control actions to the critical points in the spread of the pathogens to the consumers. 
the purpose of this study was to discover potential sources and distribution vehicles of salmonella by isolating strains from apparently healthy slaughtered food animals and wild hedgehogs and by determining the genetic relatedness between the strains and human isolates. the isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage typing, and pulsed-field gel electrophoresis with xbai and blni restriction enzymes. yet, little is known about the environmental reservoirs and predominant modes of transmission of these pathogens. antimicrobial resistance was detected in 14% of the isolates. based on our results it seems that production and wild animals can share the same salmonella serotypes and potentially transmit some of them to humans. as the humans and animals often live in close vicinity in africa and the hygiene control of the meat retail chain is defective, high salmonella carriage rates of the animals can pose a major public health risk in burkina faso. s. typhimurium isolates from poultry and humans were multiresistant to the same antimicrobials, had the same phage type dt and were closely related in pfge. of the feces samples, contained salmonella, representing a total of different serotypes. furthermore, in sub-saharan africa non-typhoidal salmonella serotypes are common bloodstream isolates in febrile patients. s. muenster isolates from hedgehogs had similar pfge patterns as the domestic animals. production and wild animals are major sources of human salmonellosis and animals raised for food also play an important role in transmission of antimicrobial resistant salmonella strains to humans. for this purpose, feces samples from apparently healthy slaughtered cattle, poultry, swine and hedgehogs were examined for the presence of salmonella enterica in burkina faso.
 production and wild animals are major sources of human salmonellosis and animals raised for food also play an important role in transmission of antimicrobial resistant salmonella strains to humans. furthermore, in sub-saharan africa non-typhoidal salmonella serotypes are common bloodstream isolates in febrile patients. yet, little is known about the environmental reservoirs and predominant modes of transmission of these pathogens. the purpose of this study was to discover potential sources and distribution vehicles of salmonella by isolating strains from apparently healthy slaughtered food animals and wild hedgehogs and by determining the genetic relatedness between the strains and human isolates. for this purpose, feces samples from apparently healthy slaughtered cattle, poultry, swine and hedgehogs were examined for the presence of salmonella enterica in burkina faso. the isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage typing, and pulsed-field gel electrophoresis with xbai and blni restriction enzymes.  of the feces samples, contained salmonella, representing a total of different serotypes. salmonella was present in 52% of the cattle, 55% of the poultry, 16% of the swine and 96% of the hedgehog feces samples. antimicrobial resistance was detected in 14% of the isolates. s. typhimurium isolates from poultry and humans were multiresistant to the same antimicrobials, had the same phage type dt and were closely related in pfge. s. muenster isolates from hedgehogs had similar pfge patterns as the domestic animals.  based on our results it seems that production and wild animals can share the same salmonella serotypes and potentially transmit some of them to humans. as the humans and animals often live in close vicinity in africa and the hygiene control of the meat retail chain is defective, high salmonella carriage rates of the animals can pose a major public health risk in burkina faso. this underlines the necessity for a joint and coordinated surveillance and monitoring programs for salmonellosis in africa. salmonellaserotypesantimicrobial resistancegenetic relatednesspfge 