BACKGROUND
gut bacteria have been shown to play a critical role in multiple physiological changes of the host related to metabolic disorders, immunity, brain development and many other aspects  <cit> .
a lot of factors, including the diet and the age of host, can affect the composition of gut bacteria and subsequently host health.
in human and model animal studies, aging-related physiological changes in the gastrointestinal tract, e.g., inflammation and immunosenescence have been shown associated with the composition of gut microbiota .
immunosenescence, a dysregulation of the immune system with age, may be related to antigen load from gut microbiota and affect the homeostatic balance of the gut microbiota  <cit> .
in many cases, meat dish is one of the main courses for consumers over a large age span from the young to the adult and even to the elderly because meat is believed to be a good source of high-quality protein and other nutrients  <cit> .
however, epidemiological studies have shown that human bodies may give different responses to meat intake with age  <cit> , and its underlying mechanism needs further study.
human nutrition studies are sometimes difficult to realize because of complex individual variations in background gut bacteria, behavior control and high cost, and model animal studies are the good choices and have been widely used.
in a model animal study, we found that the intake of meat protein, especially chicken protein, may promote the growth of the beneficial genus lactobacillus and maintain a more balanced composition of gut bacteria in growing rats as compared to soy protein diet  <cit> .
however, it is still less known whether intake of chicken protein has the same impact on gut bacteria of middle-aged rats.
the present study compared the composition of fecal microbiota between young and middle-aged rats after being fed chicken protein diet, and investigated whether intake of chicken protein  had the similar effectiveness to promote growth of beneficial gut bacteria in middle-aged rats to that in growing rats.
methods
animals and diets
a total of  <dig> male sprague-dawley rats were used, including eight growing rats  from the zhejiang experimental animal center , and seven middle age rats  from the academy of military medical sciences experimental animal center .
the animal age phases were evaluated according to flurkey, currer and harrison   <cit> .
these two centers have the similar facility and quality control systems approved by the bureau of quality and technical supervision, p.r.
china and the rats from the centers have the same backgrounds.
in this case, the differences between the two centers were minimized.
the rats were reared in a specific pathogen-free  animal center .
the ethical committee of the experimental animal center of nanjing agricultural university approved the experimental protocol.
all rats were acclimatized for 7 days  with a new environment that had a 12 h light-dark cycle and constant temperature and humidity .
after acclimatization, all rats were fed the formulated chicken protein diet.
the animals were individually housed in plastic cages and given water and diet ad libitum.
animal diets were formulated according to the recommendation of the american institute of nutrition   <cit> .
the diets were composed of chicken protein powder , cornstarch , dextrinized cornstarch , sucrose , soybean oil , fiber , mineral mix , vitamin mix , choline bitartrate , tert-butylhydroquinone  and moisture .
protein powder was extracted from chicken pectoralis major muscle as previously described  <cit> , and the protein percent was  <dig> %.
sample collection
feces were collected from growing and middle-aged rats on day  <dig> and day  <dig>  all fecal samples were immediately frozen in liquid nitrogen and then transferred to −80 °c for analyses of gut bacteria composition.
bacterial community analyses and bioinformatics
dna was extracted using the qiaamp dna stool mini kit  according to the manufacturer’s protocol.
the v4-v <dig> region of the 16s rrna gene was selected for amplification from dna samples.
the universal primers used were f <dig>  and r <dig>  which also carried an eight-base unique sequence  for each sample.
pcr reactions were run and amplicons were sequenced as previously described  <cit> .
bioinformatics analysis was referred to our previous study  <cit> .
raw fastq files were demultiplexed and quality-filtered using qiime .
briefly, the 250 bp reads were truncated at any site receiving an average quality score < <dig> over a 10 bp sliding window.
the truncated reads shorter than 50 bp were removed.
exact barcode matching was defined with not more than 2 bp mismatching with primer.
reads containing ambiguous characters were removed.
the sequences that overlap longer than 10 bp were assembled according to their overlap sequence.
reads that could not be assembled were discarded.
operational taxonomic units  were clustered with 97% similarity cutoff using uparse  and chimeric sequences were identified and removed using uchime.
the phylogenetic affiliation of each 16s rrna gene sequence was analyzed by rdp classifier  against the silva  16s rrna database using confidence threshold of 70%.
rarefaction analysis and alpha diversity were performed using mothur.
community diversity was evaluated by shannon index and simpson index.
community richness was evaluated by chao and ace.
the heatmap was preformed by the r package .
statistical analysis
differences in the relative abundance of fecal microbiota on the phylum and genus levels between two groups at two time points were evaluated by factorial analysis of variance .
means were compared by duncan’s multiple comparison under the sas system .
significance was set below  <dig>  for all statistical analyses.
lefse analysis was performed  to find the the differences between two or more biological conditions   <cit> .
the differences in features were identified at the otu level.
the lefse analysis conditions were as follows: 1) alpha value for the factorial kruskal-wallis test among classes was less than  <dig> ; 2) alpha value for the pairwise wilcoxon test among subclasses was less than  <dig> ; 3) the threshold on the logarithmic lda score for discriminative features was less than  <dig> ; 4) multi-class analysis was set as all-against-all.
RESULTS
richness and diversity of gut microbiota
there were  <dig>  usable raw reads and  <dig> species-level operation taxonomy units  from all  <dig> samples.
the averages were  <dig>  ±  <dig> reads and 332 ±  <dig> otus per biological sample .
there was no significant difference in the number of usable raw reads between the diet and age groups .
on day  <dig>  the numbers of otus did not differ  between the young and middle-aged groups; however, the middle-aged group had fewer otus than the young group on day  <dig> .
although the rarefaction curves did not reach a stable state , the shannon-wiener diversity estimates from all the samples became stable , indicating that microbial diversity did not change too much during growth.
no significant differences  existed between the young and middle-aged groups at the two time points in ace, chao, shannon, simpson and good’s coverage indices for gut bacteria .fig.
<dig> diversity estimation of gut bacteria in rat feces.
a average number of usable raw reads ; b average number of otus at the 97% similarity level ; c rarefaction curves at the 97% similarity level.
each curve represents one biological sample; d shannon–wiener diversity index curves at the 97% similarity level.
each curve represents one biological sample
overall profiling of gut microbiota
multivariate analyses were performed to discriminate fecal samples of gut bacteria for otu.
principal component analysis  showed age-dependent structural rearrangement and a substantial inter- and intra- variation of gut bacteria as a response to diet shift .
the composition of gut bacteria showed differences between young and middle-aged rat samples at two time points.
diet changes resulted in a greater alteration in the composition of gut bacteria for the middle-aged rats; however, it was less pronounced in the young rats.fig.
<dig> otu principal component analysis of gut bacteria
fig.
<dig> relative abundances of gut bacteria by phylum and genus.
a phylum; b genus
composition of gut microbiota
at the phylum level, firmicutes and bacteroidetes were the two predominant phyla .
on day  <dig>  firmicutes accounted for  <dig> % ±  <dig> % of the total abundance of gut bacteria, while  <dig> % ±  <dig> % for bacteroidetes in the middle-aged group.
for young rats, the relative abundances of firmicutes and bacteroidetes were  <dig> % ±  <dig> % and  <dig> % ±  <dig> %, respectively.
on day  <dig>  the percentage of firmicutes decreased to  <dig> % ± 18% for the middle-aged rats and increased to  <dig> % ±  <dig> % for young rats .
however, the percentage of bacteroidetes increased to  <dig> %  for the middle-aged rats and decreased to  <dig> % for the young rats .
this indicated that the baselines of the phyla firmicutes and bacteroidetes were quite different and they had opposite responses to the intake of chicken protein.
in addition, the young group had higher relative abundances of fusobacteria compared with the middle-aged group at the two time points .
chicken protein intake induced a greater increase in the relative abundance of spirochaetae in the young group ; however, there was no influence on the middle-aged group .fig.
<dig> specific phylotypes of gut bacteria in response to age using lefse.
a day 0; b day  <dig>  the left histogram shows the lda scores computed for features at the otu level.
the right heatmap shows the relative abundance of otu .
each column represents one animal and each row represents the otu corresponding to left one.
the color intensity scale showed the relative abundance of otu , yellow denotes an high relative abundance of otu while black denotes a low relative abundance of otu
at the genus level, the composition of gut bacteria was significantly different between the young and middle-aged groups in response to diet and age .
on day  <dig>  the middle-aged group had higher relative abundances of lactobacillus, s24_ <dig> norank and rf <dig> norank, and lower relative abundances of fusobacterium, alloprevotella, prevotellaceae uncultured, peptostreptococcaceae incertaes, roseburia, treponema, anaerobiospirillum, anaerotruncus and quinella compared with the young rat group.
the relative abundance of bacteroides  was lower in the middle-aged group .
on day  <dig>  the relative abundance of bacteroides was increased to  <dig> % in the middle-aged group  and decreased to  <dig> % in the young group.
however, the relative abundance of lactobacillus was the opposite, which significantly decreased in the middle-aged group  and increased in the young group .
the middle-aged group had a higher abundance of phascolarctobacterium  after being fed chicken protein.
however, no significant change occurred in the young group .
chicken protein diet decreased the relative abundance of blautia in both young and middle-aged rats.table  <dig> relative abundance of primary genera in rat feces
lactobacillus
s24-7_norank
ruminococcaceae_uncultured
bacteroides
prevotellaceae_uncultured
fusobacterium
blautia
alloprevotella
phascolarctobacterium
rf9_norank
lachnospiraceae_unclassified
escherichia-shigella
peptostreptococcaceae_incertae_sedis
treponema
ruminococcaceae_unclassified
ruminococcus
defluviitaleaceae_uncultured
allobaculum
specific phylotypes varying with age and diet
pca and anova test revealed changes in the composition of gut microbiota in response to age and diet.
to identify specific phylotypes for age and diet, linear discriminant analysis effect size  was performed on the otu level with a relative abundance that was at least more than 1% in one group.
age effect
at baseline ,  <dig> otus were significantly different between the young and middle-aged groups .
the relative abundances of  <dig> otus were higher in the middle-aged group, including the genus lactobacillus , s24- <dig> norank  and blautia .
however,  <dig> otus were more abundant in the young group, including the genus bacteroides  and fusobacterium .fig.
<dig> specific phylotypes of gut bacteria in response to diet shift using lefse.
a young group; b middle-aged group.
the left histogram shows the lda scores computed for features at the otu level.
the right heatmap shows the relative abundance of otu .
each column represents one animal and each row represents the otu corresponding to left one.
the color intensity scale showed the relative abundance of otu , yellow denotes an high relative abundance of otu while black denotes a low relative abundance of otu
on day  <dig>   <dig> otus were significantly different between the middle-aged and young groups .
thirteen otus had higher relative abundances in the middle-aged group, including the genera s24- <dig> norank, lactobacillus and blautia.
the other  <dig> otus were highly abundant in the young group, including the genera fusobacterium, alloprevotella, and prevotellaceae_uncultured.
diet effect
for the young rats, only  <dig> otus showed significant responses to diet .
the relative abundances of three otus increased with the diet shift, including the genera lactobacillus  and ruminococcus .
the other three otus showed negative responses to the diet shift, including the genera bacteroides , phascolarctobacterium , and anaerobiospirillum .
for the middle-aged rats,  <dig> otus had positive or negative responses to the diet change .
thirteen of them were positive, including the genus bacteroides  and several other genera.
eighteen were negative, including the genera lactobacillus , s24- <dig> norank  and blautia .
discussion
the ratio of the phylum firmicutes to the phylum bacteroidetes could be associated with obesity  <cit> .
this ratio showed a positive response to diet change from normal chow  to formulated chicken protein diet for the young group, but a negative response was observed for the middle-aged rats.
however, none of rats showed obese characteristics.
this could be attributed to two aspects:  chicken protein in the diet may down regulate lipid metabolism pathway in liver  <cit> ;  it is specific bacteria strains but not the phyla that contribute to obesity and other physiological responses  <cit> .
for example, the lactobacillus gasseri strain has an anti-obesity effect in overweight and obese people  <cit> .
moreover, some studies even showed lower ratios of the phylum firmicutes to the phylum bacteroidetes in obese individuals  <cit> .
lactobacilli have the ability to convert lactose and other sugars into lactic acid and have been used to treat and prevent diarrhea  <cit> .
in addition, lactobacillus plays an important role in host health and immune function due to their potential to inhibit the growth of pathogens and to prevent intestinal disorders  <cit> .
therefore, the intake of chicken protein might decrease the functions of lactobacillus in middle-aged rats.
however, other aspects should be considered.
for example, serum lipopolysaccharide binding protein is usually considered a biomarker for an inflammatory response and an antigen load to the host  <cit> .
this needs to be further studied.
bacteroides have genes encoding hydrolases of soluble polysaccharides and have the capability of utilizing various substrates  <cit> .
firmicutes typically carry fewer genes for polysaccharide degradation  <cit> .
however, a previous fermentation study showed that human gut bacteria could degrade insoluble starch particles and wheat bran, and these bacteria belonged to the phylum firmicutes but not the phylum bacteroides  <cit> .
the species ruminococcus bromii, in the phylum firmicutes, plays a key role in the degradation of resistant starch  <cit> .
in fact, the genes encoding hydrolases for polysaccharide degradation in firmicutes were less studied than those in bacteroides.
therefore, it is difficult to associate the relative abundance of phylotypes in the genus bacteroides with the level of polysaccharide degradation.
it needs further study.
changes in the composition of gut microbiota could be one of age-related physiological processes.
previous studies showed that a high similarity of the gut bacterial community in the elderly to that of younger adults indicated a high probability for the elderly to be relatively healthy  <cit> .
however, it could be more important for the host to have the ability to establish a new balance for the age-related bacteria.
in the study, gut microbiota showed different responses to the intake of chicken protein between young and middle-aged rats.
a formulated chicken protein diet  caused a reduction in the phylum firmicutes and an increase in the bacteroides for middle-aged rats, with the opposite results for young rats.
in addition, the relative abundance of the genus lactobacillus increased for young rats after they were fed chicken protein for 14 days, which may reduce the antigen load and inflammatory response from gut bacteria to the host.
however, the abundance of the genus lactobacillus decreased in middle-aged rats, which may affect host health.
finally, the capacities of protein digestion and absorption in the small intestine may different between young and middle ages  <cit> .
this would cause more undigested or unabsorbed dietary proteins to enter into the large intestine and alter the gut bacteria composition.
CONCLUSIONS
our results showed that gut microbiota could be changed by  <dig> % chicken protein diet and the effects were significantly different between middle-aged and young rats.
chicken protein intake promoted the beneficial genus lactobacillus in young rats, but an opposite effect was observed in the middle-aged group.
the association between meat protein intake and gut microbiota in middle-aged rats needs to be further studied.
meanwhile, to evaluate the association between diet and host health, age effect should be considered in future studies.
additional file
additional file 1:
table s <dig>  richness and diversity indexes relative to each sample.
table s <dig>  the differentially fecal bacterial communities between young-0d and middle aged-0d using lefse at the otu level.
table s <dig>  the differentially fecal bacterial communities between young-14d and middle aged-14d using lefse at the otu level.
table s <dig>  the differentially fecal bacterial communities between young-0d and young-14d using lefse at the otu level.
table s <dig>  the differentially fecal bacterial communities between middle aged-0d and middle aged-14d using lefse at the otu level.
abbreviations
anovaanalysis of variance
lefselinear discriminant analysis effect size
otuoperation taxonomy units
pcaprincipal component analysis

 gut bacteria have been shown to play a critical role in multiple physiological changes of the host related to metabolic disorders, immunity, brain development and many other aspects. a lot of factors, including the diet and the age of host, can affect the composition of gut bacteria and subsequently host health. in human and model animal studies, aging-related physiological changes in the gastrointestinal tract, e.g., inflammation and immunosenescence have been shown associated with the composition of gut microbiota. immunosenescence, a dysregulation of the immune system with age, may be related to antigen load from gut microbiota and affect the homeostatic balance of the gut microbiota. in many cases, meat dish is one of the main courses for consumers over a large age span from the young to the adult and even to the elderly because meat is believed to be a good source of high-quality protein and other nutrients. however, epidemiological studies have shown that human bodies may give different responses to meat intake with age, and its underlying mechanism needs further study. human nutrition studies are sometimes difficult to realize because of complex individual variations in background gut bacteria, behavior control and high cost, and model animal studies are the good choices and have been widely used. in a model animal study, we found that the intake of meat protein, especially chicken protein, may promote the growth of the beneficial genus lactobacillus and maintain a more balanced composition of gut bacteria in growing rats as compared to soy protein diet. however, it is still less known whether intake of chicken protein has the same impact on gut bacteria of middle-aged rats. the present study compared the composition of fecal microbiota between young and middle-aged rats after being fed chicken protein diet, and investigated whether intake of chicken protein had the similar effectiveness to promote growth of beneficial gut bacteria in middle-aged rats to that in growing rats. methods animals and diets a total of male sprague-dawley rats were used, including eight growing rats from the zhejiang experimental animal center, and seven middle age rats from the academy of military medical sciences experimental animal center. the animal age phases were evaluated according to flurkey, currer and harrison. these two centers have the similar facility and quality control systems approved by the bureau of quality and technical supervision, p.r. china and the rats from the centers have the same backgrounds. in this case, the differences between the two centers were minimized. the rats were reared in a specific pathogen-free animal center. the ethical committee of the experimental animal center of nanjing agricultural university approved the experimental protocol. all rats were acclimatized for 7 days with a new environment that had a 12 h light-dark cycle and constant temperature and humidity. after acclimatization, all rats were fed the formulated chicken protein diet. the animals were individually housed in plastic cages and given water and diet ad libitum. animal diets were formulated according to the recommendation of the american institute of nutrition. the diets were composed of chicken protein powder, cornstarch, dextrinized cornstarch, sucrose, soybean oil, fiber, mineral mix, vitamin mix, choline bitartrate, tert-butylhydroquinone and moisture. protein powder was extracted from chicken pectoralis major muscle as previously described, and the protein percent was %. sample collection feces were collected from growing and middle-aged rats on day and day all fecal samples were immediately frozen in liquid nitrogen and then transferred to −80 °c for analyses of gut bacteria composition. bacterial community analyses and bioinformatics dna was extracted using the qiaamp dna stool mini kit according to the manufacturer’s protocol. the v4-v region of the 16s rrna gene was selected for amplification from dna samples. the universal primers used were f and r which also carried an eight-base unique sequence for each sample. pcr reactions were run and amplicons were sequenced as previously described. bioinformatics analysis was referred to our previous study. raw fastq files were demultiplexed and quality-filtered using qiime. briefly, the 250 bp reads were truncated at any site receiving an average quality score < over a 10 bp sliding window. the truncated reads shorter than 50 bp were removed. exact barcode matching was defined with not more than 2 bp mismatching with primer. reads containing ambiguous characters were removed. the sequences that overlap longer than 10 bp were assembled according to their overlap sequence. reads that could not be assembled were discarded. operational taxonomic units were clustered with 97% similarity cutoff using uparse and chimeric sequences were identified and removed using uchime. the phylogenetic affiliation of each 16s rrna gene sequence was analyzed by rdp classifier against the silva 16s rrna database using confidence threshold of 70%. rarefaction analysis and alpha diversity were performed using mothur. community diversity was evaluated by shannon index and simpson index. community richness was evaluated by chao and ace. the heatmap was preformed by the r package. statistical analysis differences in the relative abundance of fecal microbiota on the phylum and genus levels between two groups at two time points were evaluated by factorial analysis of variance. means were compared by duncan’s multiple comparison under the sas system. significance was set below for all statistical analyses. lefse analysis was performed to find the the differences between two or more biological conditions. the differences in features were identified at the otu level. the lefse analysis conditions were as follows: 1) alpha value for the factorial kruskal-wallis test among classes was less than; 2) alpha value for the pairwise wilcoxon test among subclasses was less than; 3) the threshold on the logarithmic lda score for discriminative features was less than; 4) multi-class analysis was set as all-against-all.  richness and diversity of gut microbiota there were usable raw reads and species-level operation taxonomy units from all samples. the averages were  ±  reads and 332 ±  otus per biological sample. there was no significant difference in the number of usable raw reads between the diet and age groups. on day the numbers of otus did not differ between the young and middle-aged groups; however, the middle-aged group had fewer otus than the young group on day. although the rarefaction curves did not reach a stable state, the shannon-wiener diversity estimates from all the samples became stable, indicating that microbial diversity did not change too much during growth. no significant differences existed between the young and middle-aged groups at the two time points in ace, chao, shannon, simpson and good’s coverage indices for gut bacteria.fig. diversity estimation of gut bacteria in rat feces. a average number of usable raw reads; b average number of otus at the 97% similarity level; c rarefaction curves at the 97% similarity level. each curve represents one biological sample; d shannon–wiener diversity index curves at the 97% similarity level. each curve represents one biological sample overall profiling of gut microbiota multivariate analyses were performed to discriminate fecal samples of gut bacteria for otu. principal component analysis showed age-dependent structural rearrangement and a substantial inter- and intra- variation of gut bacteria as a response to diet shift. the composition of gut bacteria showed differences between young and middle-aged rat samples at two time points. diet changes resulted in a greater alteration in the composition of gut bacteria for the middle-aged rats; however, it was less pronounced in the young rats.fig. otu principal component analysis of gut bacteria fig. relative abundances of gut bacteria by phylum and genus. a phylum; b genus composition of gut microbiota at the phylum level, firmicutes and bacteroidetes were the two predominant phyla. on day firmicutes accounted for % ±  % of the total abundance of gut bacteria, while % ±  % for bacteroidetes in the middle-aged group. for young rats, the relative abundances of firmicutes and bacteroidetes were % ±  % and % ±  %, respectively. on day the percentage of firmicutes decreased to % ± 18% for the middle-aged rats and increased to % ±  % for young rats. however, the percentage of bacteroidetes increased to % for the middle-aged rats and decreased to % for the young rats. this indicated that the baselines of the phyla firmicutes and bacteroidetes were quite different and they had opposite responses to the intake of chicken protein. in addition, the young group had higher relative abundances of fusobacteria compared with the middle-aged group at the two time points. chicken protein intake induced a greater increase in the relative abundance of spirochaetae in the young group; however, there was no influence on the middle-aged group.fig. specific phylotypes of gut bacteria in response to age using lefse. a day 0; b day the left histogram shows the lda scores computed for features at the otu level. the right heatmap shows the relative abundance of otu. each column represents one animal and each row represents the otu corresponding to left one. the color intensity scale showed the relative abundance of otu, yellow denotes an high relative abundance of otu while black denotes a low relative abundance of otu at the genus level, the composition of gut bacteria was significantly different between the young and middle-aged groups in response to diet and age. on day the middle-aged group had higher relative abundances of lactobacillus, s24_ norank and rf norank, and lower relative abundances of fusobacterium, alloprevotella, prevotellaceae uncultured, peptostreptococcaceae incertaes, roseburia, treponema, anaerobiospirillum, anaerotruncus and quinella compared with the young rat group. the relative abundance of bacteroides was lower in the middle-aged group. on day the relative abundance of bacteroides was increased to % in the middle-aged group and decreased to % in the young group. however, the relative abundance of lactobacillus was the opposite, which significantly decreased in the middle-aged group and increased in the young group. the middle-aged group had a higher abundance of phascolarctobacterium after being fed chicken protein. however, no significant change occurred in the young group. chicken protein diet decreased the relative abundance of blautia in both young and middle-aged rats.table relative abundance of primary genera in rat feces lactobacillus s24-7_norank ruminococcaceae_uncultured bacteroides prevotellaceae_uncultured fusobacterium blautia alloprevotella phascolarctobacterium rf9_norank lachnospiraceae_unclassified escherichia-shigella peptostreptococcaceae_incertae_sedis treponema ruminococcaceae_unclassified ruminococcus defluviitaleaceae_uncultured allobaculum specific phylotypes varying with age and diet pca and anova test revealed changes in the composition of gut microbiota in response to age and diet. to identify specific phylotypes for age and diet, linear discriminant analysis effect size was performed on the otu level with a relative abundance that was at least more than 1% in one group. age effect at baseline, otus were significantly different between the young and middle-aged groups. the relative abundances of otus were higher in the middle-aged group, including the genus lactobacillus, s24- norank and blautia. however, otus were more abundant in the young group, including the genus bacteroides and fusobacterium.fig. specific phylotypes of gut bacteria in response to diet shift using lefse. a young group; b middle-aged group. the left histogram shows the lda scores computed for features at the otu level. the right heatmap shows the relative abundance of otu. each column represents one animal and each row represents the otu corresponding to left one. the color intensity scale showed the relative abundance of otu, yellow denotes an high relative abundance of otu while black denotes a low relative abundance of otu on day otus were significantly different between the middle-aged and young groups. thirteen otus had higher relative abundances in the middle-aged group, including the genera s24- norank, lactobacillus and blautia. the other otus were highly abundant in the young group, including the genera fusobacterium, alloprevotella, and prevotellaceae_uncultured. diet effect for the young rats, only otus showed significant responses to diet. the relative abundances of three otus increased with the diet shift, including the genera lactobacillus and ruminococcus. the other three otus showed negative responses to the diet shift, including the genera bacteroides, phascolarctobacterium, and anaerobiospirillum. for the middle-aged rats, otus had positive or negative responses to the diet change. thirteen of them were positive, including the genus bacteroides and several other genera. eighteen were negative, including the genera lactobacillus, s24- norank and blautia. discussion the ratio of the phylum firmicutes to the phylum bacteroidetes could be associated with obesity. this ratio showed a positive response to diet change from normal chow to formulated chicken protein diet for the young group, but a negative response was observed for the middle-aged rats. however, none of rats showed obese characteristics. this could be attributed to two aspects: chicken protein in the diet may down regulate lipid metabolism pathway in liver; it is specific bacteria strains but not the phyla that contribute to obesity and other physiological responses. for example, the lactobacillus gasseri strain has an anti-obesity effect in overweight and obese people. moreover, some studies even showed lower ratios of the phylum firmicutes to the phylum bacteroidetes in obese individuals. lactobacilli have the ability to convert lactose and other sugars into lactic acid and have been used to treat and prevent diarrhea. in addition, lactobacillus plays an important role in host health and immune function due to their potential to inhibit the growth of pathogens and to prevent intestinal disorders. therefore, the intake of chicken protein might decrease the functions of lactobacillus in middle-aged rats. however, other aspects should be considered. for example, serum lipopolysaccharide binding protein is usually considered a biomarker for an inflammatory response and an antigen load to the host. this needs to be further studied. bacteroides have genes encoding hydrolases of soluble polysaccharides and have the capability of utilizing various substrates. firmicutes typically carry fewer genes for polysaccharide degradation. however, a previous fermentation study showed that human gut bacteria could degrade insoluble starch particles and wheat bran, and these bacteria belonged to the phylum firmicutes but not the phylum bacteroides. the species ruminococcus bromii, in the phylum firmicutes, plays a key role in the degradation of resistant starch. in fact, the genes encoding hydrolases for polysaccharide degradation in firmicutes were less studied than those in bacteroides. therefore, it is difficult to associate the relative abundance of phylotypes in the genus bacteroides with the level of polysaccharide degradation. it needs further study. changes in the composition of gut microbiota could be one of age-related physiological processes. previous studies showed that a high similarity of the gut bacterial community in the elderly to that of younger adults indicated a high probability for the elderly to be relatively healthy. however, it could be more important for the host to have the ability to establish a new balance for the age-related bacteria. in the study, gut microbiota showed different responses to the intake of chicken protein between young and middle-aged rats. a formulated chicken protein diet caused a reduction in the phylum firmicutes and an increase in the bacteroides for middle-aged rats, with the opposite results for young rats. in addition, the relative abundance of the genus lactobacillus increased for young rats after they were fed chicken protein for 14 days, which may reduce the antigen load and inflammatory response from gut bacteria to the host. however, the abundance of the genus lactobacillus decreased in middle-aged rats, which may affect host health. finally, the capacities of protein digestion and absorption in the small intestine may different between young and middle ages. this would cause more undigested or unabsorbed dietary proteins to enter into the large intestine and alter the gut bacteria composition.  our results showed that gut microbiota could be changed by % chicken protein diet and the effects were significantly different between middle-aged and young rats. chicken protein intake promoted the beneficial genus lactobacillus in young rats, but an opposite effect was observed in the middle-aged group. the association between meat protein intake and gut microbiota in middle-aged rats needs to be further studied. meanwhile, to evaluate the association between diet and host health, age effect should be considered in future studies. additional file additional file 1: table s richness and diversity indexes relative to each sample. table s the differentially fecal bacterial communities between young-0d and middle aged-0d using lefse at the otu level. table s the differentially fecal bacterial communities between young-14d and middle aged-14d using lefse at the otu level. table s the differentially fecal bacterial communities between young-0d and young-14d using lefse at the otu level. table s the differentially fecal bacterial communities between middle aged-0d and middle aged-14d using lefse at the otu level. abbreviations anovaanalysis of variance lefselinear discriminant analysis effect size otuoperation taxonomy units pcaprincipal component analysis 
feces were collected on day and day from young rats and middle-aged rats fed with % chicken protein diets. the results showed that the composition of the gut microbiota was significantly different between young and middle-aged rats on both day and day the percentage of firmicutes decreased for middle-aged rats but increased for young rats. the percentage of bacteroidetes increased to % for middle-aged rats and decreased to % for young rats. the results of our study demonstrated that % chicken protein diet promoted the beneficial genus lactobacillus in young rats, but the opposite effect were found in the middle-aged group. to evaluate the linkage between diet and host health, age effect should be considered in the future studies. the relative abundance of the beneficial genus lactobacillus increased in response to the intake of chicken protein in the young group, while it had the opposite effect in the middle-aged group. meat protein in the diet has been shown to be beneficial for the growth of lactobacillus in the caecum of growing rats; however, it is unknown whether gut microbiota in middle-aged animals have the same responses to meat protein diets. the composition of the gut bacteria was analyzed by sequencing the v4-v region of the 16s ribosomal rna gene. this study compared the composition of the gut microbiota between young and middle-aged rats after being fed % chicken protein diet for 14 days. gut microbiotaagingchicken proteinold ratshttp://dx.doi.org/ /501100001809national natural science foundation of china3147160031530054li chunbao issue-copyright-statement© the author 2016
 meat protein in the diet has been shown to be beneficial for the growth of lactobacillus in the caecum of growing rats; however, it is unknown whether gut microbiota in middle-aged animals have the same responses to meat protein diets. this study compared the composition of the gut microbiota between young and middle-aged rats after being fed % chicken protein diet for 14 days. methods feces were collected on day and day from young rats and middle-aged rats fed with % chicken protein diets. the composition of the gut bacteria was analyzed by sequencing the v4-v region of the 16s ribosomal rna gene.  the results showed that the composition of the gut microbiota was significantly different between young and middle-aged rats on both day and day the percentage of firmicutes decreased for middle-aged rats but increased for young rats. the percentage of bacteroidetes increased to % for middle-aged rats and decreased to % for young rats. the relative abundance of the beneficial genus lactobacillus increased in response to the intake of chicken protein in the young group, while it had the opposite effect in the middle-aged group.  the results of our study demonstrated that % chicken protein diet promoted the beneficial genus lactobacillus in young rats, but the opposite effect were found in the middle-aged group. to evaluate the linkage between diet and host health, age effect should be considered in the future studies. electronic supplementary material the online version of this article contains supplementary material, which is available to authorized users. keywords gut microbiotaagingchicken proteinold ratshttp://dx.doi.org/ /501100001809national natural science foundation of china3147160031530054li chunbao issue-copyright-statement© the author 2016 