BACKGROUND
margarine was originally developed in  <dig> as an alternative to butter which was in short supply and was also expensive  <cit> .
margarine is a water-in-oil emulsion.
the aqueous phase consists of water, salt and preservatives.
the fatty phase, which contributes to the polymorphic behaviour of margarine, is a blend of oils and fats, antioxidants and emulsifiers.
traditionally, the solid fat content of margarine is obtained by hydrogenation of liquid oils.
hydrogenation results in the formation of trans fatty acids where some cis double bonds are rearranged to trans bonds  <cit> .
several studies have suggested a direct relationship between trans fatty acids and increased risk for coronary heart diseases as well as raise of plasmatic lipid levels  <cit> .
different processes are currently available for the production of zero-trans solid fats in the food industry including chemical  <cit>  or enzymatic transesterification  <cit> .
chemical transesterification usually needs cleaning process to remove the residual catalyst besides the formation of undesirable products.
it is being successfully replaced by enzymatic processes of modifying fats and oils by utilizing lipases from various origins  <cit> .
enzymatic conversion of fats has been reported by various researchers  <cit> .
ghazali et al.
<cit>  have conducted in hexane media the enzymatic transesterification of palm olein with nonspecific and  <dig> -specific lipases immobilized on celite.
the effects of transesterification of palm olein by the various lipases resulted in changes in the triglycerides mixture and no clear correlation between the enzyme positional specificity and the products formed was found.
palm oil is extracted from the fruit of oil palm, elaeis guineensis.
it is one of the traditional fats that have been widely used throughout the world in the human diet.
global palm oil production was estimated to  <dig>  millions of tons during 2009– <dig>  accounting for 40 % of the worldwide production of total dietary oils  <cit> .
palm oil contains a mixture of high and low melting points triacylglycerols.
using a simple dry fractionation process under controlled conditions, palm oil can be resolved into two fractions, namely olein  and stearin   <cit> .
palm olein is rich in low melting point triacylglycerols and is the mostly used fraction  <cit> .
however, the high melting point fraction  is hardly used in manufacturing edible fats due to its low plasticity  <cit> .
nevertheless, palm stearin deserves attention as a potential hard fat of vegetable origin to replace hydrogenated lipids.
it might be appropriately blended and interesterified with liquid oils in order to modify the physical characteristics of the mixture to meet the functional properties and the quality required for margarine preparation.
lai et al.
<cit>  have used nonspecific and  <dig> -specific lipases to transesterify mixtures of palm stearin and sunflower oil at a 40: <dig> mass ratio in a solvent-free medium.
the authors have found that the palm stearin and sunflower oil mixtures were converted to a more fluid product.
in the same context, lai et al.
<cit>  have also transesterified a mixture of palm stearin and palm kernel olein using the same lipases.
they reported that the enzymatic transesterification was able to produce fat mixtures with substantially lower melting points by repositioning the fatty acids of triglycerides in the higher melting range to form lower-or middle-melting components.
in the same context, this work reports the synthesis of a fatty phase by transesterification of palm stearin and palm olein using an immobilized rhizopus oryzae lipase as a biocatalyst.
the maximal rate of palm stearin that is usually added to a standard table margarine formulation is 10%  <cit> .
the purpose of this work was to maximise the palm stearin proportion in the fatty phase .
a margarine was prepared out of the palm stearin/palm olein interesterified  mixture used as fatty phase.
slip melting point and rheological properties of the margarine were studied.
in order to improve the colour, powder of dry bark orange was added to one margarine sample which rheological properties were studied.
methods
production and immobilization of lipase
rhizopus oryzae lipase was produced as described by ben salah et al.
<cit> .
the enzyme immobilization was made onto caco <dig> as described by ghamgui et al.
<cit> .
the activity of the immobilized lipase was measured titrimetrically with a ph-stat, under the standard assay conditions described previously by rathelot et al.
<cit>  using olive oil  emulsion as substrate.
one international unit  of lipase activity was defined as the amount of lipase that catalyzes the liberation of 1 μmol of fatty acid per minute at ph  <dig>  and 37 °c.
fractionation process
refined, bleached and deodorized palm oil, of iodine value  <dig>  was obtained from the tunisian olive oil office.
it was fractionated in the laboratory by a dry fractionation process, according to the method described by thiagarajah  <cit> .
rbd palm oil was melted and kept homogenized at 70°c to destroy all crystals present.
the melted oil was stirred at 25 rpm to keep it homogenized.
the temperature was then decreased to 30°c.
after stabilization, two fractions were obtained, a solid fraction: palm stearin  and a liquid fraction: palm olein .
they were separated by vacuum filtration.
enzymatic transesterification
transesterification reactions using various palm stearine/palm olein  mixtures  were conducted in screw-capped flasks containing 10 g of total lipids.
reactions were monitored for 72 hours at 50 °c using 1000 iu of immobilized lipase and under stirring .
the biocatalyst was removed from reaction samples by centrifugation at 8000 rpm for 5 min, washed thoroughly with hexane and reused in the reusability study and the supernatant was used for determining the melting point or the triacylglycerols composition.
a control experiment was carried out in the same conditions without adding the enzyme.
slip melting point
smp was determined according to the aocs method cc.
<dig>   <cit> .
capillary tubes filled each with 1 cm high column of fat were chilled in a refrigerator at 4°c before being immersed in a beaker of cold distilled water.
the water was stirred and heated and the temperature was recorded when the column of fat rises in the tube.
iodine value by wijs method  and acid value
the iodine value and the acid value were determined according to the aocs method cd- <dig> and cd 3a- <dig>  respectively  <cit> .
the reported values are means of three measurements.
triacylglycerols  profiles
the tgs profiles of the transesterified and non-transesterified blends of ps:po were analyzed using a reversed-phase high performance liquid chromatography  equipped with a refractive index detector and with two c <dig> reverse phase analytical shim-pack clc-ods  columns connected in series for a good separation  and the second .
during analysis, the column was maintained at 45 °c.
the mobile phase was a mixture of acetone/ acetonitrile at a ratio of 70: <dig>  and at a flow rate of  <dig>  ml/min.
identification of tgs was done by comparison of retention times with those of commercial tgs standards.
fatty acid analysis
samples were dissolved in  <dig>  ml of hexane.
then,  <dig>  ml of potassium hydroxide in methanol  was added for the fatty acid methylation process.
the mixture was vortexed then centrifuged and the upper phase containing fatty acid methyl esters were analyzed by gas chromatography .
gc analyses were performed on a shimadzu, gc  <dig> a chromatograph, equipped with a flame ionization detector and a capillary column .
the oven temperature was programmed as follows: the initial temperature  was raised to 150°c at a rate of 30°c/min and held at this temperature for 5 min.
the temperature was then increased to 190°c  and maintained for 14 min before being increased  to 255°c and held for 10 min.
the injector and detector temperatures were  <dig> and 270°c, respectively.
nitrogen was the carrier gas with a flow rate of  <dig>  ml/min.
the identification of fatty acids was achieved by comparing retention times with those of authentic standards analysed under the same conditions.
peak areas were measured with an hp computing integrator.
results which are means of triplicates were expressed as w/w percentage of total fatty acids  <cit> .
margarine formulation and preparation
unless otherwise indicated, the composition of the prepared margarine was: 81% transesterified fat,  <dig> % water, 1% hard boiled egg yolk used as emulsifier,  <dig> % sugar,  <dig> % salt and 3% butter.
hard boiled egg yolk and butter were dissolved in the heated oil phase , and sugar and salt were dissolved in the water phase.
an overhead stirrer  was used to homogenize the margarine samples.
both phases were stirred and cooled rapidly in order to obtain small and uniform crystals  <cit> .
three margarine samples were prepared using the transesterified fat at a ps/po mass ratio of 40/ <dig>  one margarine sample contained no butter and 84% of fat.
the second contained 3% of butter.
the third sample contained 3% of butter and  <dig> % of a powder of dry bark orange.
a control margarine sample was prepared using non transesterified ps/po mixture at a ratio of 40/ <dig>  w/w.
commercial margarine containing hydrogenated fat was also studied.
it was purchased from a local supermarket in sfax, tunisia.
rheological analysis
for all margarine samples, viscosity was followed at10°c with a stress tech rheologica rheometer  conducted with a steel cone-plate .
RESULTS
fractionation of palm oil
dry fractionation process was applied to separate palm oil into two fractions, olein and stearin, without the addition of chemicals or solvents.
the dry fractionation is based on differences in melting points of triacylglycerols  <cit> , and is a thermomechanical separation process where the high and low melting triacylglycerols are separated by partial crystallization, followed by filtration  <cit> .
iodine value  reflects the unsaturation level of fats and oils.
among oil palm fractions, palm olein had a higher iv and lower slip melting point as compared to stearin.
this is in agreement with its higher content in unsaturated fatty acids.
changes in slip melting points during transesterification
transesterification is used to modify the properties of triacylglycerol mixtures.
the fatty acid chains are redistributed within the triacylglycerol molecules resulting generally in a change in the melting characteristics of the product in comparison with the starting mixture  <cit> .
in a preliminary study , we have checked the transesterification performance of staphylococcus xylosus and rhizopus oryzae lipases produced in our laboratory  <cit>  and immobilized onto caco <dig> <cit>  and chirazyme® l- <dig> a commercial immobilized lipase from rhizomucor miehei.
these enzymes were tested in the transesterification of a 50: <dig> ps/po  mixture.
upon a reaction time of 24 h a significant shift in the smp was obtained for the rol- and chirazyme -catalyzed reactions .
however, no change in smp was observed for s. xylosus- lipase-catalyzed reaction.
these results are presumably related to the specificity of the rol toward long-chain triacylglycerols  <cit>  as compared to s. xylosus lipase more active on short-chain triacylglycerols  <cit> , since ps/po mixture is rich in long chain fatty acids.
in addition, caco <dig> was used as a support to immobilize rol by adsorption.
the choice of this support was based on results reported by ghamgui et al.
<cit>  which have tested five supports  to immobilize rol by the adsorption technique.
the authors have found that caco <dig> was the most suitable support to immobilize rol since they have obtained a high yield of immobilization .
moreover, the most widely used lipases in the synthesis reaction were commercial lipases which are usually microbial extracellular enzymes produced by fermentation of yeasts, fungi or bacteria.
unfortunately, the utilization of commercial enzymes to perform the transesterification is still very expensive.
the use of low-cost lipases like rhizopus oryzae lipase  may increase the process economical and environmental attractiveness.
no previous studies involving rol in the transesterification reaction to produce fat phase suitable for margarine preparation were reported.
the slip melting points  of three blends containing various palm stearin / palm olein  ratios  decreased during transesterification time course .
this is likely to be explained by the rearrangement of fatty acids within triacylglycerols  using rol as a biocatalyst.
no changes were observed in the smp for control experiments carried out without enzyme.
after 48 h of reaction time, the smp remained constant.
this might indicate that the reaction reached the equilibrium.
these results are in agreement with the decrease in the smp of palm stearin/palm kernel olein mixture after transesterification using pseudomonas lipase  <cit> .
the smp increases as the amounts of ps augment in the starting blends , due to a greater content in high-melting triacylglycerols in ps.
smp of ps/po  decreased after 24 h of reaction time from 41 °c to 37 °c.
this suggests a possible usage of this blend in the preparation of a table margarine for which a slip melting point around body temperature is required for a proper mouthfeel.
furthermore, stopping the reaction at 24 h would allow to reduce the reaction cost.
this blend was subjected to further analysis in order to be used in margarine formulation.
to check the quality of the blend at the end of the reaction, the acid value was determined.
it is defined by woodlat  <cit>  as the number of mg of koh required to neutralize 1 g of fatty acids in an oil.
as shown in table  <dig>  the acid value increased slightly from the starting blend .
such value is still acceptable and there is no need to further refine the final product.
changes in triacylglycerol profiles during transesterification
ps/po  mixture was analyzed by hplc to determine the triacylglycerols profile before  and after 24 h of enzymatic transesterification .
prior to transesterification, the blends contained higher proportions of high melting point triacylglycerols, such as pop , pos , and ppp .
after transesterification, the amounts of these saturated tgs decreased and lower melting point tgs such as loo , lnlno , and ooo  were formed .
the changes in the triacylglycerols composition were due to the rearrangement of the fatty acids during interesterification.
these results are in agreement with previous findings  <cit>  that transesterification of fats and oil blends was often accompanied by changes in the smp.
preparation of margarines and rheological analysis
the ps/po  blend transesterified with 1000 iu of roli for 24 h of reaction time was used as a fatty phase to produce table margarine samples which were prepared in compliance with the margarine formulation recommendations  <cit> .
hard boiled egg yolk  was used as emulsifier.
egg yolk contains about 10% of phospholipids which are desirable emulsifiers widely used in food formulations  <cit> .
egg yolk was boiled in order to avoid any risk of product contamination.
figure  <dig> shows three margarine samples.
the first was prepared with non transesterified blend  and the two others were prepared with transesterified blend .
in order to give the product a butter-like attractive colour, powder of dry bark orange was added to the last margarine preparation .
this powder used as a source of carotenes gave to the margarine sample a yellow-orange colour, very close to that of butter.
a patent describing this formulation has been filed with the patent office  <cit> .
table margarine must be spreadable when taken straight from the refrigerator.
that’s why all rheological analysis were performed at 10°c; temperature of refrigerator’s butter compartment.
τs which represents also a measure of margarine spreadability was determined.
margarines prepared with transesterified fat had a better spreadability than those prepared with a non transesterified blend.
furthermore, the spreadability of transesterified fat margarine was similar to that of a commercial product.
since butter might be added to a margarine preparation at a maximal rate of 3%  <cit> , we checked that adding 3% of butter to the zero trans fat margarine did not affect its rheological behaviour.
the rheological behaviour of the margarine prepared with powder of dry bark orange was fairly similar to the commercial product.
reusability of the biocatalyst
one important factor limiting the use of lipase catalyzed reactions at an industrial scale is the high enzyme cost which can determine the economic viability of any biosynthetic process  <cit> .
the cost efficiency of the reaction could be greatly improved by reusing the lipase for several reactions  <cit> .
after 24 h of reaction time, the same immobilized enzyme was reused many times.
after a reaction cycle, the immobilized lipase was separated from the reaction mixture by centrifugation, washed thoroughly with hexane and a fresh substrate sample was added to the same enzyme.
no significant decrease in the enzyme activity was observed for 4 cycles of reuse and the residual activity was 90%.
upon the fifth cycle, the enzyme lost 50% of its initial activity .
the reason for the reduction of enzyme activity can be partly explained by desorption of lipase from the adsorbent or denaturation after many cycles of use.
these results indicate the feasibility of enzyme recycling in this system.
we used in a previous study  <cit>  the same biocatalyst for 20 cycles in the synthesis of wax esters without significant loss of its activity.
this difference between the two reactions could be explained by the composition of the reaction medium  or the operating conditions.
CONCLUSIONS
this study has shown that enzymatic transesterification was an effective way to modify the physical and chemical properties of palm stearin and palm olein blends.
the enzymatic transesterification allows to obtain fats with optimum melting characteristics for use in margarine production.
the rheological analysis showed that margarine prepared with the transesterified blend showed a better spreadability than that of a control margarine prepared with non transesterified fat.
adding powder of dry bark orange to margarine preparation improved its colour and fairly affected its spreadability and rheological behaviour.
competing interests
the authors declare that they have no competing interests.
authors’ contributions
ms, hg and ff designed the experiments, carried out the synthesis and the analysis of margarine and drafted the manuscript.yg and nm have conceived research and approaches and have given final approval of the version to be published.
all authors read and approved the final manuscript.
acknowledgments
this work received financial support from “ministère de l’enseignement supérieur et de la recherche scientifique, tunisie” granted to the laboratoire de biochimie et de génie enzymatique des lipases.

 margarine was originally developed in as an alternative to butter which was in short supply and was also expensive. margarine is a water-in-oil emulsion. the aqueous phase consists of water, salt and preservatives. the fatty phase, which contributes to the polymorphic behaviour of margarine, is a blend of oils and fats, antioxidants and emulsifiers. traditionally, the solid fat content of margarine is obtained by hydrogenation of liquid oils. hydrogenation results in the formation of trans fatty acids where some cis double bonds are rearranged to trans bonds. several studies have suggested a direct relationship between trans fatty acids and increased risk for coronary heart diseases as well as raise of plasmatic lipid levels. different processes are currently available for the production of zero-trans solid fats in the food industry including chemical or enzymatic transesterification. chemical transesterification usually needs cleaning process to remove the residual catalyst besides the formation of undesirable products. it is being successfully replaced by enzymatic processes of modifying fats and oils by utilizing lipases from various origins. enzymatic conversion of fats has been reported by various researchers. ghazali et al. have conducted in hexane media the enzymatic transesterification of palm olein with nonspecific and -specific lipases immobilized on celite. the effects of transesterification of palm olein by the various lipases resulted in changes in the triglycerides mixture and no clear correlation between the enzyme positional specificity and the products formed was found. palm oil is extracted from the fruit of oil palm, elaeis guineensis. it is one of the traditional fats that have been widely used throughout the world in the human diet. global palm oil production was estimated to millions of tons during 2009– accounting for 40 % of the worldwide production of total dietary oils. palm oil contains a mixture of high and low melting points triacylglycerols. using a simple dry fractionation process under controlled conditions, palm oil can be resolved into two fractions, namely olein and stearin. palm olein is rich in low melting point triacylglycerols and is the mostly used fraction. however, the high melting point fraction is hardly used in manufacturing edible fats due to its low plasticity. nevertheless, palm stearin deserves attention as a potential hard fat of vegetable origin to replace hydrogenated lipids. it might be appropriately blended and interesterified with liquid oils in order to modify the physical characteristics of the mixture to meet the functional properties and the quality required for margarine preparation. lai et al. have used nonspecific and -specific lipases to transesterify mixtures of palm stearin and sunflower oil at a 40: mass ratio in a solvent-free medium. the authors have found that the palm stearin and sunflower oil mixtures were converted to a more fluid product. in the same context, lai et al. have also transesterified a mixture of palm stearin and palm kernel olein using the same lipases. they reported that the enzymatic transesterification was able to produce fat mixtures with substantially lower melting points by repositioning the fatty acids of triglycerides in the higher melting range to form lower-or middle-melting components. in the same context, this work reports the synthesis of a fatty phase by transesterification of palm stearin and palm olein using an immobilized rhizopus oryzae lipase as a biocatalyst. the maximal rate of palm stearin that is usually added to a standard table margarine formulation is 10%. the purpose of this work was to maximise the palm stearin proportion in the fatty phase. a margarine was prepared out of the palm stearin/palm olein interesterified mixture used as fatty phase. slip melting point and rheological properties of the margarine were studied. in order to improve the colour, powder of dry bark orange was added to one margarine sample which rheological properties were studied. methods production and immobilization of lipase rhizopus oryzae lipase was produced as described by ben salah et al.. the enzyme immobilization was made onto caco as described by ghamgui et al.. the activity of the immobilized lipase was measured titrimetrically with a ph-stat, under the standard assay conditions described previously by rathelot et al. using olive oil emulsion as substrate. one international unit of lipase activity was defined as the amount of lipase that catalyzes the liberation of 1 μmol of fatty acid per minute at ph and 37 °c. fractionation process refined, bleached and deodorized palm oil, of iodine value was obtained from the tunisian olive oil office. it was fractionated in the laboratory by a dry fractionation process, according to the method described by thiagarajah. rbd palm oil was melted and kept homogenized at 70°c to destroy all crystals present. the melted oil was stirred at 25 rpm to keep it homogenized. the temperature was then decreased to 30°c. after stabilization, two fractions were obtained, a solid fraction: palm stearin and a liquid fraction: palm olein. they were separated by vacuum filtration. enzymatic transesterification transesterification reactions using various palm stearine/palm olein mixtures were conducted in screw-capped flasks containing 10 g of total lipids. reactions were monitored for 72 hours at 50 °c using 1000 iu of immobilized lipase and under stirring. the biocatalyst was removed from reaction samples by centrifugation at 8000 rpm for 5 min, washed thoroughly with hexane and reused in the reusability study and the supernatant was used for determining the melting point or the triacylglycerols composition. a control experiment was carried out in the same conditions without adding the enzyme. slip melting point smp was determined according to the aocs method cc.. capillary tubes filled each with 1 cm high column of fat were chilled in a refrigerator at 4°c before being immersed in a beaker of cold distilled water. the water was stirred and heated and the temperature was recorded when the column of fat rises in the tube. iodine value by wijs method and acid value the iodine value and the acid value were determined according to the aocs method cd- and cd 3a- respectively. the reported values are means of three measurements. triacylglycerols profiles the tgs profiles of the transesterified and non-transesterified blends of ps:po were analyzed using a reversed-phase high performance liquid chromatography equipped with a refractive index detector and with two c reverse phase analytical shim-pack clc-ods columns connected in series for a good separation and the second. during analysis, the column was maintained at 45 °c. the mobile phase was a mixture of acetone/ acetonitrile at a ratio of 70: and at a flow rate of  ml/min. identification of tgs was done by comparison of retention times with those of commercial tgs standards. fatty acid analysis samples were dissolved in  ml of hexane. then,  ml of potassium hydroxide in methanol was added for the fatty acid methylation process. the mixture was vortexed then centrifuged and the upper phase containing fatty acid methyl esters were analyzed by gas chromatography. gc analyses were performed on a shimadzu, gc a chromatograph, equipped with a flame ionization detector and a capillary column. the oven temperature was programmed as follows: the initial temperature was raised to 150°c at a rate of 30°c/min and held at this temperature for 5 min. the temperature was then increased to 190°c and maintained for 14 min before being increased to 255°c and held for 10 min. the injector and detector temperatures were and 270°c, respectively. nitrogen was the carrier gas with a flow rate of  ml/min. the identification of fatty acids was achieved by comparing retention times with those of authentic standards analysed under the same conditions. peak areas were measured with an hp computing integrator. results which are means of triplicates were expressed as w/w percentage of total fatty acids. margarine formulation and preparation unless otherwise indicated, the composition of the prepared margarine was: 81% transesterified fat, % water, 1% hard boiled egg yolk used as emulsifier, % sugar, % salt and 3% butter. hard boiled egg yolk and butter were dissolved in the heated oil phase, and sugar and salt were dissolved in the water phase. an overhead stirrer was used to homogenize the margarine samples. both phases were stirred and cooled rapidly in order to obtain small and uniform crystals. three margarine samples were prepared using the transesterified fat at a ps/po mass ratio of 40/ one margarine sample contained no butter and 84% of fat. the second contained 3% of butter. the third sample contained 3% of butter and % of a powder of dry bark orange. a control margarine sample was prepared using non transesterified ps/po mixture at a ratio of 40/ w/w. commercial margarine containing hydrogenated fat was also studied. it was purchased from a local supermarket in sfax, tunisia. rheological analysis for all margarine samples, viscosity was followed at10°c with a stress tech rheologica rheometer conducted with a steel cone-plate.  fractionation of palm oil dry fractionation process was applied to separate palm oil into two fractions, olein and stearin, without the addition of chemicals or solvents. the dry fractionation is based on differences in melting points of triacylglycerols, and is a thermomechanical separation process where the high and low melting triacylglycerols are separated by partial crystallization, followed by filtration. iodine value reflects the unsaturation level of fats and oils. among oil palm fractions, palm olein had a higher iv and lower slip melting point as compared to stearin. this is in agreement with its higher content in unsaturated fatty acids. changes in slip melting points during transesterification transesterification is used to modify the properties of triacylglycerol mixtures. the fatty acid chains are redistributed within the triacylglycerol molecules resulting generally in a change in the melting characteristics of the product in comparison with the starting mixture. in a preliminary study, we have checked the transesterification performance of staphylococcus xylosus and rhizopus oryzae lipases produced in our laboratory and immobilized onto caco and chirazyme® l- a commercial immobilized lipase from rhizomucor miehei. these enzymes were tested in the transesterification of a 50: ps/po mixture. upon a reaction time of 24 h a significant shift in the smp was obtained for the rol- and chirazyme -catalyzed reactions. however, no change in smp was observed for s. xylosus- lipase-catalyzed reaction. these results are presumably related to the specificity of the rol toward long-chain triacylglycerols as compared to s. xylosus lipase more active on short-chain triacylglycerols, since ps/po mixture is rich in long chain fatty acids. in addition, caco was used as a support to immobilize rol by adsorption. the choice of this support was based on results reported by ghamgui et al. which have tested five supports to immobilize rol by the adsorption technique. the authors have found that caco was the most suitable support to immobilize rol since they have obtained a high yield of immobilization. moreover, the most widely used lipases in the synthesis reaction were commercial lipases which are usually microbial extracellular enzymes produced by fermentation of yeasts, fungi or bacteria. unfortunately, the utilization of commercial enzymes to perform the transesterification is still very expensive. the use of low-cost lipases like rhizopus oryzae lipase may increase the process economical and environmental attractiveness. no previous studies involving rol in the transesterification reaction to produce fat phase suitable for margarine preparation were reported. the slip melting points of three blends containing various palm stearin / palm olein ratios decreased during transesterification time course. this is likely to be explained by the rearrangement of fatty acids within triacylglycerols using rol as a biocatalyst. no changes were observed in the smp for control experiments carried out without enzyme. after 48 h of reaction time, the smp remained constant. this might indicate that the reaction reached the equilibrium. these results are in agreement with the decrease in the smp of palm stearin/palm kernel olein mixture after transesterification using pseudomonas lipase. the smp increases as the amounts of ps augment in the starting blends, due to a greater content in high-melting triacylglycerols in ps. smp of ps/po decreased after 24 h of reaction time from 41 °c to 37 °c. this suggests a possible usage of this blend in the preparation of a table margarine for which a slip melting point around body temperature is required for a proper mouthfeel. furthermore, stopping the reaction at 24 h would allow to reduce the reaction cost. this blend was subjected to further analysis in order to be used in margarine formulation. to check the quality of the blend at the end of the reaction, the acid value was determined. it is defined by woodlat as the number of mg of koh required to neutralize 1 g of fatty acids in an oil. as shown in table the acid value increased slightly from the starting blend. such value is still acceptable and there is no need to further refine the final product. changes in triacylglycerol profiles during transesterification ps/po mixture was analyzed by hplc to determine the triacylglycerols profile before and after 24 h of enzymatic transesterification. prior to transesterification, the blends contained higher proportions of high melting point triacylglycerols, such as pop, pos, and ppp. after transesterification, the amounts of these saturated tgs decreased and lower melting point tgs such as loo, lnlno, and ooo were formed. the changes in the triacylglycerols composition were due to the rearrangement of the fatty acids during interesterification. these results are in agreement with previous findings that transesterification of fats and oil blends was often accompanied by changes in the smp. preparation of margarines and rheological analysis the ps/po blend transesterified with 1000 iu of roli for 24 h of reaction time was used as a fatty phase to produce table margarine samples which were prepared in compliance with the margarine formulation recommendations. hard boiled egg yolk was used as emulsifier. egg yolk contains about 10% of phospholipids which are desirable emulsifiers widely used in food formulations. egg yolk was boiled in order to avoid any risk of product contamination. figure shows three margarine samples. the first was prepared with non transesterified blend and the two others were prepared with transesterified blend. in order to give the product a butter-like attractive colour, powder of dry bark orange was added to the last margarine preparation. this powder used as a source of carotenes gave to the margarine sample a yellow-orange colour, very close to that of butter. a patent describing this formulation has been filed with the patent office. table margarine must be spreadable when taken straight from the refrigerator. that’s why all rheological analysis were performed at 10°c; temperature of refrigerator’s butter compartment. τs which represents also a measure of margarine spreadability was determined. margarines prepared with transesterified fat had a better spreadability than those prepared with a non transesterified blend. furthermore, the spreadability of transesterified fat margarine was similar to that of a commercial product. since butter might be added to a margarine preparation at a maximal rate of 3%, we checked that adding 3% of butter to the zero trans fat margarine did not affect its rheological behaviour. the rheological behaviour of the margarine prepared with powder of dry bark orange was fairly similar to the commercial product. reusability of the biocatalyst one important factor limiting the use of lipase catalyzed reactions at an industrial scale is the high enzyme cost which can determine the economic viability of any biosynthetic process. the cost efficiency of the reaction could be greatly improved by reusing the lipase for several reactions. after 24 h of reaction time, the same immobilized enzyme was reused many times. after a reaction cycle, the immobilized lipase was separated from the reaction mixture by centrifugation, washed thoroughly with hexane and a fresh substrate sample was added to the same enzyme. no significant decrease in the enzyme activity was observed for 4 cycles of reuse and the residual activity was 90%. upon the fifth cycle, the enzyme lost 50% of its initial activity. the reason for the reduction of enzyme activity can be partly explained by desorption of lipase from the adsorbent or denaturation after many cycles of use. these results indicate the feasibility of enzyme recycling in this system. we used in a previous study the same biocatalyst for 20 cycles in the synthesis of wax esters without significant loss of its activity. this difference between the two reactions could be explained by the composition of the reaction medium or the operating conditions.  this study has shown that enzymatic transesterification was an effective way to modify the physical and chemical properties of palm stearin and palm olein blends. the enzymatic transesterification allows to obtain fats with optimum melting characteristics for use in margarine production. the rheological analysis showed that margarine prepared with the transesterified blend showed a better spreadability than that of a control margarine prepared with non transesterified fat. adding powder of dry bark orange to margarine preparation improved its colour and fairly affected its spreadability and rheological behaviour. competing interests the authors declare that they have no competing interests. authors’ contributions ms, hg and ff designed the experiments, carried out the synthesis and the analysis of margarine and drafted the manuscript.yg and nm have conceived research and approaches and have given final approval of the version to be published. all authors read and approved the final manuscript. acknowledgments this work received financial support from “ministère de l’enseignement supérieur et de la recherche scientifique, tunisie” granted to the laboratoire de biochimie et de génie enzymatique des lipases.
the rheological analysis showed that margarine prepared with the transesterified blend showed a better spreadability than that of a control margarine prepared with non transesterified fat. palm stearin and palm olein blends in different ratios were enzymatically transesterified in a solvent free system using a rhizopus oryzae lipase immobilised onto caco to produce a suitable fat for margarine formulation. this study is an ecofriendly approach to the utilization of relatively low value bioresources like palm stearin and palm olein for making margarine free of trans fatty acids that are now implicated as risk factor for heart diseases. slip melting points and triacylglycerols profiles were evaluated upon transesterification. furthermore, the triacylglycerols profile showed a decrease in the concentration of the high melting point triacylglycerols. results indicated that all transesterified blends had lower slip melting points than their non transesterified counterparts. adding powder of dry bark orange to margarine preparation improved its colour and fairly affected its spreadability and rheological behaviour. the margarine prepared with transesterified fat displayed a rheological behaviour that was comparable to that of commercial sample. enzymatic transesterification can be a technological option to produce trans free fats targeting commercial applications. food industries aim to replace trans fat in their products by formulations having equivalent functionality and economic viability.
 food industries aim to replace trans fat in their products by formulations having equivalent functionality and economic viability. enzymatic transesterification can be a technological option to produce trans free fats targeting commercial applications.  palm stearin and palm olein blends in different ratios were enzymatically transesterified in a solvent free system using a rhizopus oryzae lipase immobilised onto caco to produce a suitable fat for margarine formulation. slip melting points and triacylglycerols profiles were evaluated upon transesterification. results indicated that all transesterified blends had lower slip melting points than their non transesterified counterparts. furthermore, the triacylglycerols profile showed a decrease in the concentration of the high melting point triacylglycerols. the rheological analysis showed that margarine prepared with the transesterified blend showed a better spreadability than that of a control margarine prepared with non transesterified fat. adding powder of dry bark orange to margarine preparation improved its colour and fairly affected its spreadability and rheological behaviour. the margarine prepared with transesterified fat displayed a rheological behaviour that was comparable to that of commercial sample.  this study is an ecofriendly approach to the utilization of relatively low value bioresources like palm stearin and palm olein for making margarine free of trans fatty acids that are now implicated as risk factor for heart diseases. 