[en] The longer life expectancy and the sports injuries have increased the need to address chronic and/or orthopedic diseases which cannot be met by conventional healing techniques. Tissue Engineering, combining the research on new materials and medical techniques, appears as a promising multidisciplinary alternative which aims to address these societal challenges with the aim of achieving a better quality of life. The objective of this research is to study the feasibility of a new peg-POSS/PLLA biocomposite intended for use in the manufacture of scaffolds for cell growth and cartilage regeneration applications. The work has been divided into two parts. On the one hand, the setting of the solventcasting and electrospinning processes, and the subsequent manufacture of scaffolds by these processes. On the other hand, the characterization of both the developed composite materials and the manufactured scaffolds. Firstly, it has been carried out the physical-mechanical study of the nanocomposites consisting on the analysis of their crystalline microstructure by means of calorimetry (DSC), their thermal stability by thermogravimetric analysis (TGA), their molecular structure characterization using infrared spectroscopy (IR) and their mechanical properties by means of tensile tests. Secondly, it has been carried out the biological characterization of the manufactured scaffolds, which consisted on studying their morphology by electron microscopy (SEM), their hydrolytic biodegradation in physiological fluid, and their biocompatibility by performing a cell culture. The addition of peg-POSS nanomolecules to the PLLA has shown not to affect the processability of the material, and has increased the crystallinity, the thermal stability and the ductility, in addition to improving its resistance against biodegradation. The cell culture on the nanocomposites has been proved successful, so it can be concluded that peg-POSS has not affected the biocompatibility of the PLLA.

[eu] Bizi itxaropenaren luzapenak eta kirol lesioek teknika konbentzionalen bitartez konpondu ezin diren gaixotasun kronikoei eta/edo ortopedikoei aurre egiteko beharra areagotu dute. Ehun Ingeniaritza, material eta medikuntza teknika berrien ikerketa konbinatuz, diziplina anitzeko alternatiba itxaropentsu bezala agertu da gizartearen bizikalitate handiagoa bilatzeko erronkari erantzuteko. Ikerketa honen helburua peg-POSS/PLLA egindako biokonposite berri baten bideragarritasuna aztertzea da. Material berri hau zelula hazkuntzarako eta kartilagoaren birsorkuntzarako erabiliko diren scaffold-ak fabrikatzeko erabili ahal izango da. Lana bi zatitan banatu da. Batetik, solvent-casting eta electrospinning prozesuen konfigurazioa garatu da, eta prozesu horiek erabiliz scaffold-ak fabrikatu dira. Bestaldetik, bai material konposatuen zein scaffold-en karakterizazioa burutu da. Lehendabizi, karakterizazio fisiko-mekanikoa burutu da, nanokonpositeen mikroegitura kristalinoa kalorimetriaz (DSC), egonkortasun termikoa termograbimetriaz (TGA), egitura molekularra espektroskopia infragorriaz (IR) eta propietate mekanikoak trakzio probaz aztertu dira. Bestetik, fabrikatutako scaffold-en karakterizazio biologikoa egin da, morfologia mikroskopia elektronikoa (SEM) erabiliz, biodegradazio hidrolitikoa fluido fisiologiko batean murgilduz, eta biobateragarritasuna aztertzeko zelula-kultibo bat egin delarik. peg-POSS nanomolekulak PLLA-ri gehitzeak materialaren prozesagarritasuna ez du aldatu. Bestalde, kristalinitatea, egonkortasun termikoa, biodegradazio erresistentzia eta harikortasuna handitu ditu. Nanokonpositezko scaffold-etan egindako kultibo zelularra arrakastatsua izan da, beraz, peg-POSS-ek PLLA-ren biobateragarritasuna aldatu ez duela demostratu da.