[en] The advance that science is currently experiencing allows the development of new techniques by which the presence of a large number of emerging pollutants in the aquatic environment can be observed. The presence of these pollutants in water poses a serious danger to the functioning of ecosystems, since they would firstly attack microalgae in the first place, which are part of phytoplankton and, therefore, form the basis of ecosystems. These pollutants can be transferred through the food chain, causing significant alterations in human functioning. In the present work, the possible toxic effect of the emerging microcontaminants Bisphenol A (BP-A) and Bisphenol S (BP-S) on the freshwater microalgae Chlamydomonas reinhardtii was studied. C. reinhardtii cultures were exposed to two concentrations of both BP-A and BP-S (20 mg L-1; and 40 mg L-1) for 96 h and the parameters of cell density, cell activity and Photosynthetic activity by two routes: pigment content (chlorophyll a and b and carotenoids) and by pulse width modulation fluorimetry (MAP) against control cultures without contaminant. In this comparative study between the emerging BP-A and BP-S microcontaminants on the microalgae C. reinhardtii, a greater toxic effect of the BP-S was observed, since for all the parameters analyzed the BP-S showed significant differences (p- value <0.05) between the treatment at a concentration of 40 mg L-1 and the control, in any of the hours tested (24, 72 or 96 h). However, BP-A only caused significant inhibition (p-value <0.05) in terms of cell density at 72 and 96 h; in addition to a significant decrease (p-value <0.05) in the content of chlorophyll a and carotenoids at 24 h.

[gl] O avance que está a experimentar a ciencia na actualidade, permite desenvolver novas técnicas mediante as cales pódese observar a presencia dun gran número de contaminantes emerxentes no medio acuático. A presencia destes contaminantes na auga supón un grave perigo para o funcionamento dos ecosistemas, xa que estes atacarían en primeiro lugar ás microalgas, as cales forman parte do fitoplancto e, polo tanto, constitúen a base dos ecosistemas. Estes contaminantes poden transferirse pola cadea trófica chegando a causar importantes alteracións no funcionamento de ser humano. No presente traballo estudiouse o posible efecto tóxico dos microcontaminantes emerxentes Bisfenol A (BP-A) e Bisfenol S (BP-S) sobre a microalga doceacuícola Chlamydomonas reinhardtii. Os cultivos de C. Reinhardtii expuxéronse a dúas concentracións tanto de BP-A, como de BP-S (20 mg L-1; e 40 mg L-1) durante 96 h e analizáronse os parámetros de densidade celular, actividade celular e actividade fotosintética mediante dúas vías: contido en pigmentos (clorofila a e b e carotenos) e por fluorimetría de modulación de amplitude de pulso (PAM) fronte a cultivos control sen contaminante. Neste estudio comparativo entre os microcontaminantes emerxentes BP-A e BP-S sobre a microalga C. Reinhardtii, observouse un maior efecto tóxico do BP-S, posto que para todos os parámetros analizados o BP-S mostrou diferencias significativas (p-valor < 0,05) entre o tratamento a unha concentración de 40 mg L-1 e o control, nalgunha das horas ensaiadas (24, 72 ou 96 h). Sen embargo, o BP-A soamente provocou inhibición significativa (p-valor < 0,05) para a densidade celular ás 72 e 96 h; ademais dunha diminución significativa (p-valor < 0,05) do contido en clorofila a e carotenos ás 24 h.