[en] The environmental risk assessment of the veterinary pharmaceutical ivermectin is receiving significant attention. This paper assesses the capacity of the MS·3 soil microcosm as a tool for targeting the environmental impact assessmentof veterinary drugs, using ivermectin as model. Two screening MS·3 were performed using different European soils; one with a soil collected in an agricultural station near to Madrid, Spain and a second with a soil collected in a farm area close to York, UK. Soils were fortified with ivermectin at the following ranges: 0.01-10 mg kg–1 and 0.1-100 mg kg–1 in the Madrid and York studies, respectively. The effects on earthworms, plants and soil microorganisms were assessed in the Madrid soil. Toxicity tests on aquatic organisms (algae, cladocerans and in vitro fish cell line RTLW1) were also conducted with the leachates. No effects were observed in earthworms and plants at any tested concentration; reduction in the respiration rate (&lt; 5%) of soil microorganisms was detected. Earthworm/soilbioconcentration factors decreased with the increase in soil concentrations and were higher for the York soil. Effects on daphnids were observed in tested leachates; based on measured levels of ivermectin in the leachates an EC50 ofabout 0.5 μgL–1 can be estimated. Comparisons based on toxicity data and equilibrium partitioning confirmed that themain risk is expected to be related to the high sensitivity of cladocerans. The results confirm that MS·3 systems are cost-effective tools for assessing the impact of veterinary pharmaceuticals when applied to agricultural land, as previously demonstrated for antimicrobials.

[es] La evaluaci&oacute;n del riesgo ambiental de la ivermectina recibe atenci&oacute;n. Este trabajo considera la capacidad de microcosmosterrestres (MS&middot;3) para evaluar el impacto de medicamentos veterinarios, utilizando ivermectina como modelo. Se realizaron experimentos en MS&middot;3 con dos suelos europeos, uno de una zona agr&iacute;cola cercana a Madrid, Espa&ntilde;a y otro de una explotaci&oacute;n cerca de York, UK. Los suelos fueron&nbsp;fortificados con ivermectina considerando rangosde 0,01 a 10 mg kg&ndash;1 con suelo de Madrid y de 0,1 a 100 mg kg&ndash;1 con suelo de York. En el MS&middot;3 de Madrid se evaluaronlos efectos sobre lombrices, plantas y microorganismos. En los lixiviados, se ensay&oacute; la toxicidad en organismos acu&aacute;ticos (algas, invertebrados y ensayos in vitro, con l&iacute;neas celulares de peces RTL-W1). Las lombrices y las plantas no mostraron efecto a las concentraciones consideradas; s&iacute; se observ&oacute; reducci&oacute;n en la tasa de respiraci&oacute;n (&lt; 5%) de microorganismos. Los factores de bioconcentraci&oacute;n en lombriz disminu&iacute;an conforme aumentaba la concentraci&oacute;n de ivermectina en suelo y &eacute;stos fueron mayores en lombrices expuestas al suelo de York. Hubo efectos sobre daphnidosexpuestos a lixiviados; basado en los niveles de ivermectina medidos en lixiviados, se estim&oacute; una EC50 de 0,5&mu;g L&ndash;1. La comparaci&oacute;n entre datos de toxicidad y m&eacute;todos de equilibrio de partici&oacute;n confirma que el principal riesgo de la ivermectina debe esperarse en daphnias debido a la alta sensibilidad de clad&oacute;ceros. Los resultados confirman que los MS&middot;3 pueden considerarse una herramienta &uacute;til para la evaluaci&oacute;n de impacto ambiental de medicamentos veterinarios en suelos agr&iacute;colas, como ya se demostr&oacute; previamente con antimicrobianos.