[en] In the present study, the effect of resveratrol in rheumatoid arthritis (RA) was evaluated in vivo, in an antigen-induced arthritis (AlA) animal model, and in vitro, in cultured human arthritic synoviocytes. For the in vivo study of resveratrol effect in an AlA model in rats, resveratrol was orally administered from two months before arthritis induction until the end of the experiment, two days after the induction, coinciding with the acute phase of the disease. Resveratrol was able to reduce the hallmarks of RA pathology. In this way, it was demonstrated that resveratrol decreased synovial hyperplasia, immune ce lis infiltration into the joint and proinflammatory mediators express ion, as well as the reduction of oxidative damage through increasing antioxidant enzymes activity. AII these features was corroborated with a decrease in knee joint inflammation after arthritis induction. These results suggest that resveratrol could be a new therapeutic strategy in the treatment of RA. In the in vitro study, the effect of resveratrol in the inflammatory response induced by the cytokine IL-1~ in cultured human arthritic synoviocytes was anlyzed. It was demonstrated that resveratrol reduced the express ion of several genes involved in the inflammatory process, including those analyzed in the AlA model, thus confirming in vitro the results obtained in vivo. Gene expression of the different sirtuins was evaluated, as well as their modulation by resveratrol in human arthritic synoviocytes. It was observed that resveratrol tends to increase sirtuin 1, 2 and 3 expression under proinflammatory conditions.

[gl] Neste traballo estudouse o efecto do resveratrol na artrite reumatoide (AR) in vivo, nun modelo animal de artrite inducida por antíxeno (AlA), e in vitro, en sinoviocitos artríticos humanos en cultivo. Para o estudo in vivo do efecto do resveratrol nun modelo AlA en ratas, éste administrouse dun xeito oral durante dous meses antes da indución da artrite e ata o remate do estudo, dous días despois da indución, coincidindo coa fase aguda da enfermidade. Atopouse que o resveratrol estaba a reducir os principais signos da patoloxía da AR. Así, demostrouse como o resveratrol reduxo a hiperplasia sinovial, o infiltrado de células inmunes na articulación e a expresión dos principais mediadores inflamatorios, ademáis de diminuir o dano oxidativo mediante un aumento na actividade das enzimas antioxidantes. Todo isto viuse reflexado nunha redución da inflamación presente nos xeonllos dos animais trala indución da artrite. Estes resultados suxiren ao resveratrol como unha nova estratexa terapéutica para o trata mento da AR. No estudo in vitro, avaliouse o efecto do resveratrol na resposta inflamatoria inducida pola citocina IL-ll3 en sinoviocitos artríticos humanos en cultivo. Demostrouse como o resveratrol estaba a diminuír a expresión de numerosos xenes que participan no proceso inflamatorio, incluídos aqueles que foron avaliados no modelo AlA, corroborando así in vitro os estudos realizados in vivo. Tamén se avaliou a expresión xénica das distintas sirtuinas e a súa modulación polo resveratrol en sinoviocitos artríticos humanos, obtendo unha tendencia do resveratrol a aumentar a expresión das sirtuinas 1, 2 e 3 baixo condicións proinflamatorias.