# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 150

# Report Period 1St - 28Th February 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| February                |                          |          |
| 2019                    |                          |          |
| Submissions             | 320                      | 609      |
| Samples                 | Total (%)                | 28044    |
| England                 | 22715                    | 81.00 %  |
| Wales                   | 5329                     | 19.00 %  |
| Scotland                |                          |          |
| Parallel Tests          | PAR-CUL-N                | 4898     |
| PAR-CUL-P               | 3318                     | 11.83 %  |
| PAR-HERD-S              | 1042                     | 3.72 %   |
| PAR-NEW-IN              | 13851                    | 49.39 %  |
| PAR-OTHER               | 304                      | 1.08 %   |
| PAR-PERSIS              | 3805                     | 13.57 %  |
| PAR-RAPID               | 120                      | 0.43 %   |
| Total(% of all samples) | 27338                    | 97.48 %  |
| Serial/Extended Tests   | SER-FLEXI                | 697      |
| SER-FRAUD               | 9                        | 0.03 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 706                      | 2.52 %   |
| Total                   | 28044                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2301     |
| England                 | 1798                     | 6.41 %   |
| Wales                   | 503                      | 1.79 %   |
| Scotland                |                          |          |
| Resamples               | Total (% of all samples) | 1908     |
| England                 | 1479                     | 5.27 %   |
| Wales                   | 429                      | 1.53 %   |
| Scotland                |                          |          |
| Rejects                 | Total (% of all samples) | 221      |
| England                 | 217                      | 0.77 %   |
| Wales                   | 4                        | 0.01 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   4898 | 17.5 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   3318 | 11.8 %   |
| PAR-HERD-S  |   1042 | 3.7 %    |
| PAR-NEW-IN  |  13851 | 49.4 %   |
| PAR-OTHER   |    304 | 1.1 %    |
| PAR-PERSIS  |   3805 | 13.6 %   |
| PAR-RAPID   |    120 | 0.4 %    |
| SER-FLEXI   |    697 | 2.5 %    |
| SER-FRAUD   |      9 | 0.0 %    |
| Total       |  28044 | 100.00 % |

## Table 2A.  Summary By County For February 2019

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Avon           |
| SER-FRAUD      | 1              |
| Corn & Scilly  | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| Devon          | PAR-CUL-N      |
| PAR-CUL-P      | 9              |
| PAR-NEW-IN     | 1              |
| PAR-OTHER      | 2              |
| PAR-PERSIS     | 4              |
| SER-FLEXI      | 2              |
| Dorset         | PAR-CUL-N      |
| PAR-CUL-P      | 1              |
| Gloucs         | PAR-CUL-N      |
| Heref          | PAR-CUL-N      |
| PAR-PERSIS     | 1              |
| Shropshire     | PAR-NEW-IN     |
| PAR-OTHER      | 2              |
| PAR-PERSIS     | 3              |
| Somerset       | PAR-CUL-N      |
| Staffs         | PAR-PERSIS     |
| Wiltshire      | PAR-CUL-P      |
| PAR-PERSIS     | 1              |
| England - Edge | Berks          |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| Derbyshire     | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For February 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cambs          |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| England         |                |
| 188             | 22715          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 11             |
| PAR-RAPID       | 7              |
| SER-FLEXI       | 8              |
| Ceredigion      | PAR-HERD-S     |
| PAR-PERSIS      | 4              |
| PAR-RAPID       | 6              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 4              |
| PAR-RAPID       | 3              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 9              |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 3              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For February 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 10             |
| W Glamorgan             | PAR-RAPID      |
| Wales - Intermediate TB | NE Wales       |
| PAR-OTHER               | 2              |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 6              |
| Powys                   | PAR-NEW-IN     |
| W Glamorgan             | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| Wales - Low TB          | Gwynedd        |
| Wales                   |                |
| 132                     | 5329           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 4              |
| PAR-PERSIS    | 2              |
| SER-FLEXI     | 2              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 10             |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 9              |
| SER-FLEXI     | 4              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 3              |
| Gloucs        | PAR-CUL-N      |
| Heref         | PAR-CUL-N      |
| PAR-PERSIS    | 1              |
| Shropshire    | PAR-NEW-IN     |
| PAR-OTHER     | 4              |
| PAR-PERSIS    | 4              |
| Somerset      | PAR-CUL-N      |
| PAR-OTHER     | 2              |
| Staffs        | PAR-PERSIS     |
| Wiltshire     | PAR-CUL-P      |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| PAR-PERSIS      | 1              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| SER-NSR         | 1              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 350             | 43300          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 28             |
| PAR-RAPID       | 18             |
| SER-FLEXI       | 12             |
| Ceredigion      | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 8              |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 1              |
| Gwent                   | PAR-HERD-S     |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 11             |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 8              |
| PAR-PERSIS              | 15             |
| PAR-RAPID               | 22             |
| SER-FLEXI               | 4              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 5              |
| PAR-PERSIS              | 5              |
| PAR-RAPID               | 14             |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-HERD-S     |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| Wales - Intermediate TB | Carms          |
| Ceredigion              | PAR-HERD-S     |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 14             |
| Powys                   | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| W Glamorgan    | PAR-HERD-S     |
| PAR-OTHER      | 1              |
| Wales - Low TB | Gwynedd        |
| NE Wales       | PAR-NEW-IN     |
| Wales          |                |
| 259            | 12257          |
| Grand Total    | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol February 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 3            |
| Total           | 188          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 17           |
| Total           | 132          |
| GB              | Parallel     |
| Serial/Extended | 20           |
| Total           | 320          |
| 188.00          |              |
| 132.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 9            |
| Total           | 350          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 32           |
| Total           | 259          |
| GB              | Parallel     |
| Serial/Extended | 41           |
| Total           | 609          |
| 350.00          |              |
| 259.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (February 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
19
697
2.5%
71
10.2%
568
81.5%
69
9.9 %
44
6.3 %
14
2.0 %
Parallel - Herd or Group Slaughter
19
1042
3.7%
66
6.3%
891
85.5%
90
8.6 %
82
7.9 %
3
0.3 %
Parallel New Infection
142
13851
49.4%
687
5.0%
12091
87.3%
1163
8.4 %
955
6.9 %
118
0.9 %
Parallel - new infection cull area
34
4898
17.5%
322
6.6%
4186
85.5%
364
7.4 %
316
6.5 %
74
1.5 %
Parallel - Other
10
304
1.1%
41
13.5%
244
80.3%
29
9.5 %
18
5.9 %
1
0.3 %
Parallel Persistent Infection
42
3805
13.6%
405
10.6%
3095
81.3%
349
9.2 %
298
7.8 %
7
0.2 %
Parallel - persistent infection cull area
13
3318
11.8%
278
8.4%
2853
86.0%
225
6.8 %
183
5.5 %
4
0.1 %
Parallel - Rapid Testing Two-times IRs
40
120
0.4%
37
30.8%
71
59.2%
12
10.0 %
12
10.0 %
0
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Total
320
28044
100.0%
1907
6.8%
24008
85.6%
2301
8.2 %
1908
6.8 %
221
0.8 %
England - HRA
Extended Flexible IFN-GAMMA test
2
250
0.9%
30
12.0%
200
80.0%
22
8.8 %
6
2.4 %
14
5.6 %
Parallel New Infection
2
132
0.5%
7
5.3%
111
84.1%
13
9.8 %
14
10.6 %
0
Parallel - new infection cull area
34
4898
17.5%
322
6.6%
4186
85.5%
364
7.4 %
316
6.5 %
74
1.5 %
Parallel - Other
4
76
0.3%
15
19.7%
59
77.6%
2
2.6 %
1
1.3 %
1
1.3 %
Parallel Persistent Infection
11
1121
4.0%
182
16.2%
860
76.7%
85
7.6 %
73
6.5 %
6
0.5 %
Parallel - persistent infection cull area
13
3318
11.8%
278
8.4%
2853
86.0%
225
6.8 %
183
5.5 %
4
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - HRA
67
9804
35.0%
834
8.5%
8278
84.4%
711
7.3 %
593
6.0 %
99
1.0 %
England - Edge
Parallel New Infection
115
12176
43.4%
612
5.0%
10639
87.4%
1036
8.5 %
843
6.9 %
82
0.7 %
England - Edge
115
12176
43.4%
612
5.0%
10639
87.4%
1036
8.5 %
843
6.9 %
82
0.7 %
England - LRA
Parallel New Infection
6
735
2.6%
25
3.4%
631
85.9%
51
6.9 %
43
5.9 %
36
4.9 %
England - LRA
6
735
2.6%
25
3.4%
631
85.9%
51
6.9 %
43
5.9 %
36
4.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
11
279
1.0%
26
9.3%
229
82.1%
29
10.4 %
24
8.6 %
0
Parallel - Herd or Group Slaughter
18
1037
3.7%
65
6.3%
888
85.6%
89
8.6 %
81
7.8 %
3
0.3 %
Parallel New Infection
3
39
0.1%
5
12.8%
30
76.9%
4
10.3 %
4
10.3 %
0
Parallel - Other
3
173
0.6%
10
5.8%
147
85.0%
24
13.9 %
16
9.2 %
0
Parallel Persistent Infection
31
2684
9.6%
223
8.3%
2235
83.3%
264
9.8 %
225
8.4 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
36
103
0.4%
33
32.0%
59
57.3%
11
10.7 %
11
10.7 %
0
Wales - High TB
102
4315
15.4%
362
8.4%
3588
83.2%
421
9.8 %
361
8.4 %
4
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
6
168
0.6%
15
8.9%
139
82.7%
18
10.7 %
14
8.3 %
0
Parallel - Herd or Group Slaughter
1
5
0.0%
1
20.0%
3
60.0%
1
20.0 %
1
20.0 %
0
Parallel New Infection
13
712
2.5%
34
4.8%
631
88.6%
55
7.7 %
47
6.6 %
0
Parallel - Other
3
55
0.2%
16
29.1%
38
69.1%
3
5.5 %
1
1.8 %
0
Parallel - Rapid Testing Two-times IRs
4
17
0.1%
4
23.5%
12
70.6%
1
5.9 %
1
5.9 %
0
Wales - Intermediate TB
27
957
3.4%
70
7.3%
823
86.0%
78
8.2 %
64
6.7 %
0
Wales - Low TB
Parallel New Infection
3
57
0.2%
4
7.0%
49
86.0%
4
7.0 %
4
7.0 %
0
Wales - Low TB
3
57
0.2%
4
7.0%
49
86.0%
4
7.0 %
4
7.0 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
39
1147
2.1%
104
9.1%
884
77.1%
86
7.5 %
61
5.3 %
98
8.5 %
Parallel - Herd or Group Slaughter
35
1676
3.0%
116
6.9%
1454
86.8%
121
7.2 %
101
6.0 %
5
0.3 %
Parallel New Infection
261
27807
50.1%
1221
4.4%
24014
86.4%
2167
7.8 %
1843
6.6 %
729
2.6 %
Parallel - new infection cull area
62
7813
14.1%
490
6.3%
6642
85.0%
580
7.4 %
503
6.4 %
178
2.3 %
Parallel - Other
21
1909
3.4%
132
6.9%
1603
84.0%
161
8.4 %
132
6.9 %
42
2.2 %
Parallel Persistent Infection
90
9433
17.0%
699
7.4%
8050
85.3%
687
7.3 %
565
6.0 %
119
1.3 %
Parallel - persistent infection cull area
18
5463
9.8%
341
6.2%
4778
87.5%
407
7.5 %
338
6.2 %
6
0.1 %
Parallel - Rapid Testing Two-times IRs
81
293
0.5%
87
29.7%
166
56.7%
29
9.9 %
22
7.5 %
18
6.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
609
55557
100.0%
3190
5.7%
47607
85.7%
4238
7.6 %
3565
6.4 %
1195
2.2 %
England - HRA
Extended Flexible IFN-GAMMA test
7
329
0.6%
36
10.9%
241
73.3%
23
7.0 %
7
2.1 %
45
13.7 %
Parallel New Infection
3
476
0.9%
74
15.5%
353
74.2%
55
11.6 %
49
10.3 %
0
Parallel - new infection cull area
62
7813
14.1%
490
6.3%
6642
85.0%
580
7.4 %
503
6.4 %
178
2.3 %
Parallel - Other
9
804
1.4%
74
9.2%
639
79.5%
58
7.2 %
49
6.1 %
42
5.2 %
Parallel Persistent Infection
21
2210
4.0%
245
11.1%
1711
77.4%
179
8.1 %
151
6.8 %
103
4.7 %
Parallel - persistent infection cull area
18
5463
9.8%
341
6.2%
4778
87.5%
407
7.5 %
338
6.2 %
6
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
211
24938
44.9%
1049
4.2%
21583
86.5%
1932
7.7 %
1639
6.6 %
667
2.7 %
Parallel Persistent Infection
4
262
0.5%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - LRA
Parallel New Infection
13
989
1.8%
37
3.7%
850
85.9%
76
7.7 %
66
6.7 %
36
3.6 %
England
350
43300
77.9%
2360
5.5%
37049
85.6%
3324
7.7 %
2814
6.5 %
1077
2.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
18
402
0.7%
34
8.5%
338
84.1%
35
8.7 %
30
7.5 %
0
Parallel - Herd or Group Slaughter
32
1664
3.0%
113
6.8%
1447
87.0%
119
7.2 %
99
5.9 %
5
0.3 %
Parallel New Infection
5
46
0.1%
5
10.9%
35
76.1%
6
13.0 %
6
13.0 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Parallel - Other
8
1030
1.9%
40
3.9%
914
88.7%
94
9.1 %
76
7.4 %
0
Parallel Persistent Infection
64
6955
12.5%
440
6.3%
6097
87.7%
494
7.1 %
402
5.8 %
16
0.2 %
Parallel - Rapid Testing Two-times IRs
76
274
0.5%
83
30.3%
152
55.5%
28
10.2 %
21
7.7 %
18
6.6 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
14
416
0.7%
34
8.2%
305
73.3%
28
6.7 %
24
5.8 %
53
12.7 %
Parallel - Herd or Group Slaughter
3
12
0.0%
3
25.0%
7
58.3%
2
16.7 %
2
16.7 %
0
Parallel New Infection
20
1227
2.2%
52
4.2%
1087
88.6%
92
7.5 %
78
6.4 %
10
0.8 %
Parallel - Other
4
75
0.1%
18
24.0%
50
66.7%
9
12.0 %
7
9.3 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
5
19
0.0%
4
21.1%
14
73.7%
1
5.3 %
1
5.3 %
0
Wales - Low TB
Parallel New Infection
9
131
0.2%
4
3.1%
106
80.9%
6
4.6 %
5
3.8 %
16
12.2 %
Wales
259
12257
22.1%
830
6.8%
10558
86.1%
914
7.5 %
751
6.1 %
118
1.0 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | March   |
| April        | 195     |
| May          | 250     |
| June         | 187     |
| July         | 207     |
| August       | 228     |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| Totals (last |         |
| 12 months)   |         |
| 2865         | 268574  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1609
207166
1110
10373
2890
0
0
0
0
797
12
8
30
3
8636
15
72
1
3
Scotland
23
1615
15
128
40
0
0
0
0
22
4
0
0
0
49
0
36
0
2
Wales
1233
59793
627
3161
1740
0
0
0
0
145
6
2
21
5
2126
93
505
3
30
Totals
2865
268574
1752
13662
4670
0
0
0
0
964
22
10
51
8
10811
108
613
4
35
County
%Resampled %Retested
| Avon          | 14.29 %   | 14.29 %   |
|---------------|-----------|-----------|
| Berks         | 8.14 %    | 9.89 %    |
| Bucks         | 26.09 %   | 26.09 %   |
| Cambs         | 1.82 %    | 2.42 %    |
| Carms         | 8.10 %    | 9.44 %    |
| Ceredigion    | 10.40 %   | 11.86 %   |
| Cheshire      | 4.65 %    | 6.06 %    |
| Corn & Scilly | 9.86 %    | 9.69 %    |
| Derbyshire    | 14.27 %   | 15.85 %   |
| Devon         | 5.83 %    | 7.35 %    |
| Dorset        | 4.66 %    | 5.75 %    |
| E Sussex      | 3.25 %    | 4.34 %    |
| Gloucs        | 7.17 %    | 7.83 %    |
| Gwent         | 6.35 %    | 6.73 %    |
| Gwynedd       | 7.02 %    | 7.02 %    |
| Hampshire     | 6.40 %    | 7.06 %    |
| Heref         | 15.14 %   | 17.93 %   |
| Leics & Rut   | 8.53 %    | 9.04 %    |
| Lincs         | 30.00 %   | 30.00 %   |
| NE Wales      | 6.59 %    | 8.08 %    |
| Northants     |           |           |
| Notts         | 2.25 %    |           |
| N Yorks       | 6.61 %    | 7.86 %    |
| Oxon        | 6.53 %   | 8.57 %   |
|-------------|----------|----------|
| Pembs       | 8.40 %   | 10.18 %  |
| Powys       | 11.11 %  | 11.11 %  |
| Shropshire  | 7.86 %   | 9.29 %   |
| Somerset    | 8.40 %   | 8.40 %   |
| Staffs      | 5.88 %   | 5.88 %   |
| Warks       | 6.66 %   | 8.75 %   |
| W Glamorgan | 20.00 %  | 20.00 %  |
| Wiltshire   | 2.56 %   | 3.69 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
22388
1471
6.6%
22715
1471
6.5%
Wales
5155
436
8.5%
5329
436
8.2%
GB
27543
1907
6.9%
28044
1907
6.8%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
15261
950
6.2%
15552
950
6.1%
Wales
4941
261
5.3%
5026
261
5.2%
Scotland
213
18
8.5%
213
18
8.5%
GB
20415
1229
6.0%
20791
1229
5.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
41359
2360
5.7%
43300
2360
5.5%
Wales
11725
830
7.1%
12257
830
6.8%
GB
53084
3190
6.0%
55557
3190
5.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
29141
1607
5.5%
29772
1607
5.4%
Wales
9619
538
5.6%
10071
538
5.3%
Scotland
618
53
8.6%
626
53
8.5%
GB
39378
2198
5.6%
40469
2198
5.4%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SE
3
37
14
37.84 %
Worcestershire
SER-OTHER
1
5
0
Total
4
42
14
33.33 %
Isle of Man
SER-IOM
4
5
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Gloucestershire     | PRV-SP        |
| Somerset excl North | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 7                   |               |
| 53                  |               |
| 15                  | 28.30 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |               125 | 4                    | 3.2 %                             |
| North East Wales | 2               |       22 |                 2 | 9.1 %                |                                   |
| TC0077           | Sum:            |      147 |                 6 | 4.1 %                |                                   |
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |               125 | 4                    | 3.2 %                             |
| North East Wales | 4               |      119 |                 7 | 5.9 %                |                                   |
| TC0077           | Sum:            |      244 |                11 | 4.5 %                |                                   |