# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 159

# Report Period 1St - 30Th November 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| November                |                          |          |
| 2019                    |                          |          |
| Submissions             | 272                      | 3237     |
| Samples                 | Total (%)                | 30186    |
| England                 | 23070                    | 76.43 %  |
| Wales                   | 7111                     | 23.56 %  |
| Scotland                | 5                        | 0.02 %   |
| Parallel Tests          | PAR-CUL-N                | 5414     |
| PAR-CUL-P               | 3627                     | 12.02 %  |
| PAR-HERD-S              | 192                      | 0.64 %   |
| PAR-NEW-IN              | 12820                    | 42.47 %  |
| PAR-OTHER               | 1042                     | 3.45 %   |
| PAR-PERSIS              | 6404                     | 21.22 %  |
| PAR-RAPID               | 135                      | 0.45 %   |
| Total(% of all samples) | 29634                    | 98.17 %  |
| Serial/Extended Tests   | SER-FLEXI                | 543      |
| SER-FRAUD               | 9                        | 0.03 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 552                      | 1.83 %   |
| Total                   | 30186                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1628     |
| England                 | 1177                     | 3.90 %   |
| Wales                   | 451                      | 1.49 %   |
| Scotland                | 127                      | 0.04 %   |
| Resamples               | Total (% of all samples) | 1214     |
| England                 | 876                      | 2.90 %   |
| Wales                   | 338                      | 1.12 %   |
| Scotland                | 104                      | 0.03 %   |
| Rejects                 | Total (% of all samples) | 1410     |
| England                 | 1046                     | 3.47 %   |
| Wales                   | 364                      | 1.21 %   |
| Scotland                | 0                        | 0.00 %   |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   5414 | 17.9 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   3627 | 12.0 %   |
| PAR-HERD-S  |    192 | 0.6 %    |
| PAR-NEW-IN  |  12820 | 42.5 %   |
| PAR-OTHER   |   1042 | 3.5 %    |
| PAR-PERSIS  |   6404 | 21.2 %   |
| PAR-RAPID   |    135 | 0.4 %    |
| SER-FLEXI   |    543 | 1.8 %    |
| SER-FRAUD   |      9 | 0.0 %    |
| Total       |  30186 | 100.00 % |
Breakdown
of Samples
submitted
by County

## Table 2A.  Summary By County For November 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 8              |
| PAR-PERSIS      | 4              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| SER-FLEXI       | 1              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-NEW-IN     |
| PAR-PERSIS      | 3              |
| Shropshire      | PAR-PERSIS     |
| Somerset excl N | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 5              |
| SER-FLEXI       | 2              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Bucks          |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 16             |
| Derbyshire      | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Lincs          |
| Norfolk         | PAR-NEW-IN     |
| Suffolk         | PAR-NEW-IN     |
| England         |                |
| 164             | 23070          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 1              |
| PAR-RAPID       | 14             |
| SER-FLEXI       | 1              |
| Ceredigion      | PAR-PERSIS     |
| PAR-RAPID       | 2              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-OTHER      |
| PAR-PERSIS      | 2              |
| PAR-RAPID       | 2              |
| SER-FLEXI       | 1              |
| Pembs           | PAR-OTHER      |
| PAR-PERSIS      | 13             |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 5              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 4              |
| PAR-RAPID       | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| W Glamorgan             | PAR-OTHER      |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 3              |
| Ceredigion              | PAR-RAPID      |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 2              |
| Powys                   | PAR-NEW-IN     |
| PAR-PERSIS              | 2              |
| Shropshire              | PAR-PERSIS     |
| W Glamorgan             | PAR-PERSIS     |
| Wales - Low TB          | Gwynedd        |
| Wales                   |                |
| 107                     | 7111           |
| Scotland - Scotland     | Kirkcudbright  |
| Scotland                |                |
| 1                       | 5              |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-OTHER     | 4              |
| PAR-PERSIS    | 9              |
| SER-FRAUD     | 3              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 12             |
| PAR-NEW-IN    | 7              |
| PAR-PERSIS    | 37             |
| SER-FLEXI     | 4              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 30             |
| PAR-NEW-IN    | 4              |
| PAR-OTHER     | 7              |
| PAR-PERSIS    | 25             |
| SER-FLEXI     | 8              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 19             |
| PAR-NEW-IN    | 5              |
| SER-FLEXI     | 5              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 10             |
| PAR-HERD-S    | 4              |
| PAR-NEW-IN    | 1              |
| SER-FLEXI     | 2              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 4              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 33             |
| Shropshire      | PAR-CUL-P      |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 12             |
| SER-FLEXI       | 5              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 13             |
| Staffs          | PAR-OTHER      |
| PAR-PERSIS      | 7              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 26             |
| PAR-NEW-IN      | 7              |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 15             |
| SER-FLEXI       | 3              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 225            |
| PAR-PERSIS      | 5              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Hampshire     | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 2              |
| Leics & Rut   | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| Oxon          | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 6              |
| England - LRA | Cambs          |
| Cumbria       | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Gr London     | PAR-NEW-IN     |
| Gr Manchester | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| I of Wight    | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |
| Lancashire    | PAR-NEW-IN     |
| Lincs         | PAR-NEW-IN     |
| Norfolk       | PAR-NEW-IN     |
| Northumb      | PAR-NEW-IN     |
| PAR-PERSIS    | 3              |
| N Yorks       | PAR-NEW-IN     |
| PAR-PERSIS    | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| SER-FLEXI       | 2              |
| Suffolk         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| W Yorks         | PAR-HERD-S     |
| England         |                |
| 1911            | 244179         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 16             |
| PAR-PERSIS      | 73             |
| PAR-RAPID       | 82             |
| SER-FLEXI       | 43             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 35             |
| PAR-RAPID       | 51             |
| SER-FLEXI       | 10             |
| Gwent           | PAR-HERD-S     |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 20             |
| PAR-RAPID       | 28             |
| SER-FLEXI       | 2              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 19             |
| PAR-PERSIS      | 135            |
| PAR-RAPID       | 126            |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FLEXI               | 47             |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 30             |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 42             |
| PAR-RAPID               | 64             |
| SER-FLEXI               | 14             |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 9              |
| PAR-RAPID               | 14             |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-OTHER           | 13             |
| PAR-PERSIS          | 10             |
| PAR-RAPID           | 16             |
| SER-FLEXI           | 43             |
| Powys               | PAR-NEW-IN     |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 3              |
| Shropshire          | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| S Wales             | PAR-RAPID      |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 3              |
| PAR-RAPID           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 2              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1304                | 59902          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Kirkcudbright | PAR-CUL-P      |
| PAR-NEW-IN    | 6              |
| PAR-PERSIS    | 1              |
| Wigtown       | PAR-NEW-IN     |
| Scotland      |                |
| 22            | 2693           |
| Grand Total   | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol November 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 4            |
| Total           | 164          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 1            |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 107          |
| GB              | Parallel     |
| Serial/Extended | 14           |
| Total           | 272          |
| 164.00          |              |
| 107.00          |              |
| 1.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 35           |
| Total           | 1911 244179  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 22           |
| Wales           | Parallel     |
| Serial/Extended | 170          |
| Total           | 1304         |
| GB              | Parallel     |
| Serial/Extended | 205          |
| Total           | 3237 306774  |

####### #######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (November 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
543
1.8%
45
8.3%
465
85.6%
20
3.7 %
16
2.9 %
17
3.1 %
Parallel - Herd or Group Slaughter
6
192
0.6%
28
14.6%
145
75.5%
28
14.6 %
19
9.9 %
0
Parallel New Infection
97
12820
42.5%
378
2.9%
11548
90.1%
635
5.0 %
486
3.8 %
408
3.2 %
Parallel - new infection cull area
43
5414
17.9%
149
2.8%
4428
81.8%
257
4.7 %
192
3.5 %
645
11.9 %
Parallel - Other
10
1042
3.5%
31
3.0%
979
94.0%
44
4.2 %
32
3.1 %
0
Parallel Persistent Infection
51
6404
21.2%
299
4.7%
5445
85.0%
428
6.7 %
324
5.1 %
336
5.2 %
Parallel - persistent infection cull area
14
3627
12.0%
116
3.2%
3370
92.9%
204
5.6 %
137
3.8 %
4
0.1 %
Parallel - Rapid Testing Two-times IRs
37
135
0.4%
28
20.7%
100
74.1%
11
8.1 %
7
5.2 %
0
Serial - Anomalous Reaction
1
9
0.0%
6
66.7%
2
22.2%
1
11.1 %
1
11.1 %
0
Total
272
30186
100.0%
1080
3.6%
26482
87.7%
1628
5.4 %
1214
4.0 %
1410
4.7 %
England - HRA
Extended Flexible IFN-GAMMA test
3
299
1.0%
23
7.7%
272
91.0%
6
2.0 %
4
1.3 %
0
Parallel New Infection
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel - new infection cull area
40
4003
13.3%
127
3.2%
3096
77.3%
184
4.6 %
135
3.4 %
645
16.1 %
Parallel - Other
2
560
1.9%
4
0.7%
548
97.9%
15
2.7 %
8
1.4 %
0
Parallel Persistent Infection
20
2813
9.3%
139
4.9%
2412
85.7%
164
5.8 %
130
4.6 %
132
4.7 %
Parallel - persistent infection cull area
14
3627
12.0%
116
3.2%
3370
92.9%
204
5.6 %
137
3.8 %
4
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
6
66.7%
2
22.2%
1
11.1 %
1
11.1 %
0
England - HRA
81
11313
37.5%
415
3.7%
9702
85.8%
574
5.1 %
415
3.7 %
781
6.9 %
England - Edge
Parallel New Infection
77
9993
33.1%
324
3.2%
9007
90.1%
517
5.2 %
397
4.0 %
265
2.7 %
Parallel - new infection cull area
3
1411
4.7%
22
1.6%
1332
94.4%
73
5.2 %
57
4.0 %
0
England - Edge
80
11404
37.8%
346
3.0%
10339
90.7%
590
5.2 %
454
4.0 %
265
2.3 %
England - LRA
Parallel New Infection
3
353
1.2%
10
2.8%
336
95.2%
13
3.7 %
7
2.0 %
0
England - LRA
3
353
1.2%
10
2.8%
336
95.2%
13
3.7 %
7
2.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
8
210
0.7%
21
10.0%
177
84.3%
14
6.7 %
12
5.7 %
0
Parallel - Herd or Group Slaughter
6
192
0.6%
28
14.6%
145
75.5%
28
14.6 %
19
9.9 %
0
Parallel New Infection
1
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Parallel - Other
5
244
0.8%
22
9.0%
208
85.2%
19
7.8 %
14
5.7 %
0
Parallel Persistent Infection
24
3251
10.8%
136
4.2%
2737
84.2%
235
7.2 %
174
5.4 %
204
6.3 %
Parallel - Rapid Testing Two-times IRs
32
126
0.4%
26
20.6%
93
73.8%
11
8.7 %
7
5.6 %
0
Wales - High TB
76
4027
13.3%
233
5.8%
3364
83.5%
307
7.6 %
226
5.6 %
204
5.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
34
0.1%
1
2.9%
16
47.1%
0
0
17
50.0 %
Parallel New Infection
13
2452
8.1%
44
1.8%
2185
89.1%
103
4.2 %
80
3.3 %
143
5.8 %
Parallel - Other
3
238
0.8%
5
2.1%
223
93.7%
10
4.2 %
10
4.2 %
0
Parallel Persistent Infection
7
340
1.1%
24
7.1%
296
87.1%
29
8.5 %
20
5.9 %
0
Parallel - Rapid Testing Two-times IRs
5
9
0.0%
2
22.2%
7
77.8%
0
0
0
Wales - Intermediate TB
30
3073
10.2%
76
2.5%
2727
88.7%
142
4.6 %
110
3.6 %
160
5.2 %
Wales - Low TB
Parallel New Infection
1
11
0.0%
0
0.0 %
9
81.8%
2
18.2 %
2
18.2 %
0
Wales - Low TB
1
11
0.0%
0
0.0 %
9
81.8%
2
18.2 %
2
18.2 %
0
Scotland - Scotland
Parallel New Infection
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Scotland - Scotland
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
199
6363
2.1%
430
6.8%
5584
87.8%
317
5.0 %
231
3.6 %
118
1.9 %
Parallel - Herd or Group Slaughter
150
7534
2.5%
462
6.1%
6691
88.8%
503
6.7 %
371
4.9 %
11
0.1 %
Parallel New Infection
1187 139614
45.5%
4419
3.2%
125688
90.0%
9928
7.1 %
7808
5.6 %
1721
1.2 %
Parallel - new infection cull area
560
66128
21.6%
2917
4.4%
58394
88.3%
4687
7.1 %
3613
5.5 %
1204
1.8 %
Parallel - Other
99
5216
1.7%
309
5.9%
4438
85.1%
529
10.1 %
427
8.2 %
42
0.8 %
Parallel Persistent Infection
521
59154
19.3%
2594
4.4%
52718
89.1%
3719
6.3 %
2852
4.8 %
1002
1.7 %
Parallel - persistent infection cull area
106
21342
7.0%
1023
4.8%
19239
90.1%
1408
6.6 %
1069
5.0 %
11
0.1 %
Parallel - Rapid Testing Two-times IRs
409
1385
0.5%
250
18.1%
1031
74.4%
89
6.4 %
65
4.7 %
39
2.8 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
3237 306774
100.0%
12410
4.0%
273813
89.3%
21182
6.9 %
16438
5.4 %
4148
1.4 %
England - HRA
Extended Flexible IFN-GAMMA test
27
1933
0.6%
102
5.3%
1720
89.0%
103
5.3 %
66
3.4 %
45
2.3 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
27
2104
0.7%
139
6.6%
1757
83.5%
264
12.5 %
208
9.9 %
0
Parallel - new infection cull area
555
64419
21.0%
2874
4.5%
56804
88.2%
4593
7.1 %
3537
5.5 %
1204
1.9 %
Parallel - Other
28
2227
0.7%
92
4.1%
1831
82.2%
316
14.2 %
262
11.8 %
42
1.9 %
Parallel Persistent Infection
155
24911
8.1%
1250
5.0%
21761
87.4%
1614
6.5 %
1228
4.9 %
673
2.7 %
Parallel - persistent infection cull area
105
20990
6.8%
1016
4.8%
18899
90.0%
1400
6.7 %
1064
5.1 %
11
0.1 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
England - Edge
Parallel New Infection
888 113105
36.9%
3537
3.1%
101990
90.2%
7839
6.9 %
6138
5.4 %
1458
1.3 %
Parallel - new infection cull area
5
1709
0.6%
43
2.5%
1590
93.0%
94
5.5 %
76
4.4 %
0
Parallel Persistent Infection
18
2026
0.7%
54
2.7%
1879
92.7%
114
5.6 %
90
4.4 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
82
9717
3.2%
274
2.8%
8610
88.6%
966
9.9 %
795
8.2 %
38
0.4 %
Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

England
1911 244179
79.6%
9403
3.9%
217668
89.1%
17421
7.1 %
13560
5.6 %
3567
1.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
119
3139
1.0%
226
7.2%
2797
89.1%
148
4.7 %
113
3.6 %
3
0.1 %
Parallel - Herd or Group Slaughter
138
7376
2.4%
437
5.9%
6574
89.1%
485
6.6 %
355
4.8 %
11
0.1 %
Parallel New Infection
38
1423
0.5%
81
5.7%
1284
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
45
2130
0.7%
160
7.5%
1854
87.0%
155
7.3 %
116
5.4 %
0
Parallel Persistent Infection
309
27260
8.9%
1141
4.2%
24597
90.2%
1666
6.1 %
1291
4.7 %
233
0.9 %
Parallel - Rapid Testing Two-times IRs
355
1107
0.4%
223
20.1%
788
71.2%
81
7.3 %
57
5.1 %
39
3.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
49
1152
0.4%
97
8.4%
937
81.3%
59
5.1 %
48
4.2 %
70
6.1 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
101
9986
3.3%
277
2.8%
9056
90.7%
591
5.9 %
455
4.6 %
198
2.0 %
Parallel - Other
23
824
0.3%
56
6.8%
721
87.5%
54
6.6 %
47
5.7 %
0
Parallel Persistent Infection
29
3297
1.1%
133
4.0%
3039
92.2%
193
5.9 %
134
4.1 %
0
Parallel - Rapid Testing Two-times IRs
50
273
0.1%
27
9.9%
238
87.2%
8
2.9 %
8
2.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
31
1276
0.4%
30
2.4%
1171
91.8%
89
7.0 %
63
4.9 %
16
1.3 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.1%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1304
59902
19.5%
2917
4.9%
53656
89.6%
3634
6.1 %
2774
4.6 %
571
1.0 %
Scotland - Scotland
Parallel New Infection
20
2003
0.7%
81
4.0%
1820
90.9%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
22
2693
0.9%
90
3.3%
2489
92.4%
127
4.7 %
104
3.9 %
10
0.4 %
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2018         | December |
| 2019         | January  |
| February     | 320      |
| March        | 315      |
| April        | 269      |
| May          | 364      |
| June         | 287      |
| July         | 316      |
| August       | 262      |
| September    | 245      |
| October      | 298      |
| November     | 272      |
| Totals (last |          |
| 12 months)   |          |
| 3465         | 328328   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2033
259453
1307
10227
371
0
0
0
0
969
10
2
39
1
8519
20
71
0
1
Scotland
22
2693
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1410
66182
704
3334
137
0
0
0
0
153
7
5
18
2
2263
78
531
2
13
Totals
3465
328328
2022
13651
508
0
0
0
0
1134
17
7
59
3
10852
98
607
2
14

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Avon          | 11.11 %  | 11.11 %                |
| Bucks         | 3.10 %   | 3.49 %                 |
| Carms         | 4.48 %   | 6.99 %                 |
| Ceredigion    | 3.48 %   | 4.75 %                 |
| Cheshire      | 3.18 %   | 4.41 %                 |
| Corn & Scilly | 4.53 %   | 5.36 %                 |
| Derbyshire    | 4.79 %   | 5.90 %                 |
| Devon         | 4.55 %   | 6.34 %                 |
| Dorset        | 2.52 %   | 3.31 %                 |
| Gloucs        | 6.51 %   | 7.69 %                 |
| Gwent         | 3.87 %   | 4.42 %                 |
| Gwynedd       | 18.18 %  | 18.18 %                |
| Hampshire     |          |                        |
| Heref         | 3.19 %   | 5.32 %                 |
| Kirkcudbright |          |                        |
| Leics & Rut   | 6.92 %   | 8.36 %                 |
| Lincs         | 0.72 %   | 2.17 %                 |
| NE Wales      | 3.53 %   | 4.20 %                 |
| Norfolk       | 4.88 %   | 4.88 %                 |
| Northants     | 2.07 %   | 3.73 %                 |
| Oxon          | 4.22 %   | 5.20 %                 |
| Pembs         | 6.28 %   | 8.43 %                 |
| Powys         | 3.34 %   | 4.91 %                 |
| Shropshire      | 5.84 %   | 7.61 %   |
|-----------------|----------|----------|
| Somerset excl N | 1.13 %   | 1.39 %   |
| Suffolk         | 8.33 %   | 13.89 %  |
| Warks           | 4.61 %   | 6.35 %   |
| W Glamorgan     | 12.20 %  | 17.07 %  |
| Wiltshire       | 2.87 %   | 4.92 %   |
| Worcs           | 1.03 %   | 2.06 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
22854
771
3.4%
23070
771
3.3%
Wales
6848
309
4.5%
7111
309
4.3%
Scotland
5
0
0.0 %
5
0.0 %
GB
29707
1080
3.6%
30186
1080
3.6%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
17996
1078
6.0%
18356
1078
5.9%
Wales
6081
502
8.3%
6214
502
8.1%
GB
24076
1580
6.6%
24570
1580
6.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
215085
9403
4.4%
244179
9403
3.9%
Wales
54800
2917
5.3%
59902
2917
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
271893
12410
4.6%
306774
12410
4.0%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
154045
8796
5.7%
178364
8796
4.9%
Wales
44614
2443
5.5%
51327
2452
4.8%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
200719
11420
5.7%
231932
11429
4.9%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
2
4
0
0
Total
4
7
0
Isle of Man
SER-IOM
1
2
0
0
| % Positive                     | * Test type   |
|--------------------------------|---------------|
| Num                            |               |
| Submissions                    |               |
| Num                            |               |
| Samples                        |               |
| Num                            |               |
| Positives                      |               |
| Private                        |               |
| Devon                          | PRV-SE        |
| PRV-SP                         | 3             |
| Dorset                         | PRV-SP        |
| Gloucestershire                | PRV-SP        |
| Herefordshire                  | PRV-SP        |
| Northern Somerset & South Glou | PRV-SP        |
| Somerset excl North            | PRV-SE        |
| PRV-SP                         | 7             |
| Staffordshire                  | PRV-SE        |
| Wiltshire                      | PRV-SP        |
| Worcestershire                 | SER-OTHER     |
| Total                          |               |
| 27                             |               |
| 353                            |               |
| 19                             | 5.38 %        |
| Isle of Man                    | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                              | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077                       | Carmarthenshire |        1 |                12 | 1                    | 8.3%                              |
| Cornwall and Isles of Scilly | 1               |      237 |                30 | 12.7%                |                                   |
| North East Wales             | 3               |       78 |                 3 | 3.8%                 |                                   |
| Pembrokeshire                | 2               |      149 |                30 | 20.1%                |                                   |
| Powys                        | 1               |       13 |                 3 | 23.1%                |                                   |
| TC0077                       | Sum:            |      489 |                67 | 13.7 %               |                                   |
|                              | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077                       | Carmarthenshire | 16       |               575 | 53                   | 9.2%                              |
| Ceredigion                   | 3               | 372      |                17 | 4.6%                 |                                   |
| Cornwall and Isles of Scilly | 1               | 237      |                30 | 12.7%                |                                   |
| North East Wales             | 30              | 974      |               119 | 12.2%                |                                   |
| Pembrokeshire                | 21              | 767      |                65 | 8.5%                 |                                   |
| Powys                        | 9               | 380      |                27 | 7.1%                 |                                   |
| South Wales                  | 5               | 196      |                16 | 8.2%                 |                                   |
| Wiltshire                    | 1               | 248      |                 9 | 3.6%                 |                                   |
| TC0077                       | Sum:            | 3,749    |               336 | 9.0 %                |                                   |