# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 160

# Report Period 1St - 31St December 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| December                |                          |          |
| 2019                    |                          |          |
| Submissions             | 234                      | 3471     |
| Samples                 | Total (%)                | 25192    |
| England                 | 19595                    | 77.78 %  |
| Wales                   | 5597                     | 22.22 %  |
| Scotland                | 2693                     | 0.81 %   |
| Parallel Tests          | PAR-CUL-N                | 9518     |
| PAR-CUL-P               | 400                      | 1.59 %   |
| PAR-HERD-S              | 851                      | 3.38 %   |
| PAR-NEW-IN              | 7512                     | 29.82 %  |
| PAR-OTHER               | 578                      | 2.29 %   |
| PAR-PERSIS              | 5998                     | 23.81 %  |
| PAR-RAPID               | 127                      | 0.50 %   |
| Total(% of all samples) | 24984                    | 99.17 %  |
| Serial/Extended Tests   | SER-FLEXI                | 208      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 208                      | 0.83 %   |
| Total                   | 25192                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1500     |
| England                 | 1202                     | 4.77 %   |
| Wales                   | 298                      | 1.18 %   |
| Scotland                | 127                      | 0.04 %   |
| Resamples               | Total (% of all samples) | 1197     |
| England                 | 987                      | 3.92 %   |
| Wales                   | 210                      | 0.83 %   |
| Scotland                | 104                      | 0.03 %   |
| Rejects                 | Total (% of all samples) | 1166     |
| England                 | 1159                     | 4.60 %   |
| Wales                   | 7                        | 0.03 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   9518 | 37.8 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    400 | 1.6 %    |
| PAR-HERD-S  |    851 | 3.4 %    |
| PAR-NEW-IN  |   7512 | 29.8 %   |
| PAR-OTHER   |    578 | 2.3 %    |
| PAR-PERSIS  |   5998 | 23.8 %   |
| PAR-RAPID   |    127 | 0.5 %    |
| SER-FLEXI   |    208 | 0.8 %    |
| Total       |  25192 | 100.00 % |
Breakdown
of Samples
submitted
by County

## Table 2A.  Summary By County For December 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 4              |
| Devon           | PAR-CUL-N      |
| PAR-PERSIS      | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| SER-FLEXI       | 1              |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 2              |
| Somerset excl N | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 4              |
| SER-FLEXI       | 1              |
| England - Edge  | Bucks          |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 17             |
| PAR-PERSIS      | 1              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Leics & Rut             | PAR-NEW-IN     |
| Northants               | PAR-NEW-IN     |
| Oxon                    | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| Warks                   | PAR-NEW-IN     |
| England - LRA           | Gr Manchester  |
| N Yorks                 | PAR-NEW-IN     |
| England                 |                |
| 143                     | 19595          |
| Wales - High TB         | Carms          |
| PAR-PERSIS              | 6              |
| PAR-RAPID               | 5              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 6              |
| Gwent                   | PAR-PERSIS     |
| SER-FLEXI               | 2              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 3              |
| PAR-PERSIS              | 11             |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 3              |
| Powys                   | PAR-HERD-S     |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 2              |
| S Wales                 | PAR-HERD-S     |
| PAR-RAPID               | 1              |
| W Glamorgan             | PAR-PERSIS     |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| PAR-RAPID      | 1              |
| Mid Glamorgan  | PAR-RAPID      |
| NE Wales       | PAR-NEW-IN     |
| PAR-OTHER      | 3              |
| PAR-PERSIS     | 1              |
| PAR-RAPID      | 2              |
| SER-FLEXI      | 1              |
| S Glamorgan    | SER-FLEXI      |
| Wales - Low TB | Gwynedd        |
| NE Wales       | PAR-NEW-IN     |
| Wales          |                |
| 91             | 5597           |
| Grand Total    | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-OTHER     | 5              |
| PAR-PERSIS    | 9              |
| SER-FRAUD     | 3              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 13             |
| PAR-NEW-IN    | 7              |
| PAR-PERSIS    | 41             |
| SER-FLEXI     | 4              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 30             |
| PAR-NEW-IN    | 4              |
| PAR-OTHER     | 7              |
| PAR-PERSIS    | 28             |
| SER-FLEXI     | 8              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 20             |
| PAR-NEW-IN    | 5              |
| SER-FLEXI     | 6              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 13             |
| PAR-HERD-S    | 4              |
| PAR-NEW-IN    | 1              |
| SER-FLEXI     | 2              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 4              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 35             |
| Shropshire      | PAR-CUL-P      |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 12             |
| SER-FLEXI       | 5              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 13             |
| Staffs          | PAR-OTHER      |
| PAR-PERSIS      | 7              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 27             |
| PAR-NEW-IN      | 7              |
| PAR-OTHER       | 5              |
| PAR-PERSIS      | 19             |
| SER-FLEXI       | 4              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 242            |
| PAR-PERSIS      | 6              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Hampshire     | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 2              |
| Leics & Rut   | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| Oxon          | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 2              |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 6              |
| England - LRA | Cambs          |
| Cumbria       | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Gr London     | PAR-NEW-IN     |
| Gr Manchester | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| I of Wight    | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |
| Lancashire    | PAR-NEW-IN     |
| Lincs         | PAR-NEW-IN     |
| Norfolk       | PAR-NEW-IN     |
| Northumb      | PAR-NEW-IN     |
| PAR-PERSIS    | 3              |
| N Yorks       | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-PERSIS      | 3              |
| SER-FLEXI       | 2              |
| Suffolk         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| W Yorks         | PAR-HERD-S     |
| England         |                |
| 2054            | 263774         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 16             |
| PAR-PERSIS      | 79             |
| PAR-RAPID       | 87             |
| SER-FLEXI       | 43             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 36             |
| PAR-RAPID       | 57             |
| SER-FLEXI       | 10             |
| Gwent           | PAR-HERD-S     |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 24             |
| PAR-RAPID       | 28             |
| SER-FLEXI       | 4              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 22             |
| PAR-PERSIS      | 146            |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 133            |
| SER-FLEXI               | 50             |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 30             |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 49             |
| PAR-RAPID               | 66             |
| SER-FLEXI               | 14             |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 11             |
| PAR-RAPID               | 15             |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Mid Glamorgan       | PAR-RAPID      |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 16             |
| PAR-PERSIS          | 11             |
| PAR-RAPID           | 18             |
| SER-FLEXI           | 44             |
| Powys               | PAR-NEW-IN     |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 4              |
| Shropshire          | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| S Wales             | PAR-RAPID      |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 3              |
| PAR-RAPID           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 2              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1395                | 65499          |
| Scotland - Scotland | Argyll & Bute  |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Ayrshire      | PAR-NEW-IN     |
| Dumfries & G  | PAR-NEW-IN     |
| Kirkcudbright | PAR-CUL-P      |
| PAR-NEW-IN    | 6              |
| PAR-PERSIS    | 1              |
| Wigtown       | PAR-NEW-IN     |
| Scotland      |                |
| 22            | 2693           |
| Grand Total   | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol December 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 2            |
| Total           | 143          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 7            |
| Total           | 91           |
| GB              | Parallel     |
| Serial/Extended | 9            |
| Total           | 234          |
| 143.00          |              |
| 91.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 37           |
| Total           | 2054 263774  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 22           |
| Wales           | Parallel     |
| Serial/Extended | 177          |
| Total           | 1395         |
| GB              | Parallel     |
| Serial/Extended | 214          |
| Total           | 3471 331966  |

####### #######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
208
0.8%
30
14.4%
175
84.1%
5
2.4 %
3
1.4 %
0
Parallel - Herd or Group Slaughter
9
851
3.4%
32
3.8%
799
93.9%
33
3.9 %
20
2.4 %
0
Parallel New Infection
77
7512
29.8%
281
3.7%
6248
83.2%
453
6.0 %
354
4.7 %
629
8.4 %
Parallel - new infection cull area
52
9518
37.8%
250
2.6%
8327
87.5%
624
6.6 %
529
5.6 %
412
4.3 %
Parallel - Other
9
578
2.3%
23
4.0%
535
92.6%
26
4.5 %
19
3.3 %
1
0.2 %
Parallel Persistent Infection
47
5998
23.8%
249
4.2%
5380
89.7%
325
5.4 %
245
4.1 %
124
2.1 %
Parallel - persistent infection cull area
6
400
1.6%
15
3.8%
365
91.3%
27
6.8 %
20
5.0 %
0
Parallel - Rapid Testing Two-times IRs
25
127
0.5%
24
18.9%
96
75.6%
7
5.5 %
7
5.5 %
0
Total
234
25192
100.0%
904
3.6%
21925
87.0%
1500
6.0 %
1197
4.8 %
1166
4.6 %
England - HRA
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
2
50.0%
2
50.0 %
1
25.0 %
0
Parallel New Infection
1
30
0.1%
8
26.7%
21
70.0%
1
3.3 %
1
3.3 %
0
Parallel - new infection cull area
47
6867
27.3%
179
2.6%
5925
86.3%
410
6.0 %
351
5.1 %
412
6.0 %
Parallel - Other
2
11
0.0%
1
9.1%
10
90.9%
0
0
0
Parallel Persistent Infection
13
2765
11.0%
110
4.0%
2434
88.0%
129
4.7 %
103
3.7 %
118
4.3 %
Parallel - persistent infection cull area
6
400
1.6%
15
3.8%
365
91.3%
27
6.8 %
20
5.0 %
0
England - HRA
71
10077
40.0%
314
3.1%
8757
86.9%
569
5.6 %
476
4.7 %
530
5.3 %
England - Edge
Parallel New Infection
62
6601
26.2%
227
3.4%
5431
82.3%
396
6.0 %
314
4.8 %
629
9.5 %
Parallel - new infection cull area
5
2651
10.5%
71
2.7%
2402
90.6%
214
8.1 %
178
6.7 %
0
Parallel - Other
1
111
0.4%
7
6.3%
102
91.9%
3
2.7 %
2
1.8 %
0
Parallel Persistent Infection
1
32
0.1%
3
9.4%
20
62.5%
9
28.1 %
9
28.1 %
0
England - Edge
69
9395
37.3%
308
3.3%
7955
84.7%
622
6.6 %
503
5.4 %
629
6.7 %
England - LRA
Parallel New Infection
3
123
0.5%
1
0.8%
114
92.7%
11
8.9 %
8
6.5 %
0
England - LRA
3
123
0.5%
1
0.8%
114
92.7%
11
8.9 %
8
6.5 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
5
140
0.6%
24
17.1%
114
81.4%
3
2.1 %
2
1.4 %
0
Parallel - Herd or Group Slaughter
8
842
3.3%
32
3.8%
791
93.9%
32
3.8 %
19
2.3 %
0
Parallel - Other
3
447
1.8%
15
3.4%
416
93.1%
19
4.3 %
15
3.4 %
1
0.2 %
Parallel Persistent Infection
30
3138
12.5%
133
4.2%
2868
91.4%
185
5.9 %
131
4.2 %
6
0.2 %
Parallel - Rapid Testing Two-times IRs
21
117
0.5%
23
19.7%
88
75.2%
6
5.1 %
6
5.1 %
0
Wales - High TB
67
4684
18.6%
227
4.8%
4277
91.3%
245
5.2 %
173
3.7 %
7
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
64
0.3%
5
7.8%
59
92.2%
0
0
0
Parallel - Herd or Group Slaughter
1
9
0.0%
0
0.0 %
8
88.9%
1
11.1 %
1
11.1 %
0
Parallel New Infection
7
508
2.0%
35
6.9%
456
89.8%
27
5.3 %
17
3.3 %
0
Parallel - Other
3
9
0.0%
0
0.0 %
7
77.8%
4
44.4 %
2
22.2 %
0
Parallel Persistent Infection
3
63
0.3%
3
4.8%
58
92.1%
2
3.2 %
2
3.2 %
0
Parallel - Rapid Testing Two-times IRs
4
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Wales - Intermediate TB
20
663
2.6%
44
6.6%
596
89.9%
35
5.3 %
23
3.5 %
0
Wales - Low TB
Parallel New Infection
4
250
1.0%
10
4.0%
226
90.4%
18
7.2 %
14
5.6 %
0
Wales - Low TB
4
250
1.0%
10
4.0%
226
90.4%
18
7.2 %
14
5.6 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
208
6571
2.0%
460
7.0%
5759
87.6%
322
4.9 %
234
3.6 %
118
1.8 %
Parallel - Herd or Group Slaughter
159
8385
2.5%
494
5.9%
7490
89.3%
536
6.4 %
391
4.7 %
11
0.1 %
Parallel New Infection
1264 147126
44.3%
4700
3.2%
131936
89.7%
10381
7.1 %
8162
5.5 %
2350
1.6 %
Parallel - new infection cull area
612
75646
22.8%
3167
4.2%
66721
88.2%
5311
7.0 %
4142
5.5 %
1616
2.1 %
Parallel - Other
108
5794
1.7%
332
5.7%
4973
85.8%
555
9.6 %
446
7.7 %
43
0.7 %
Parallel Persistent Infection
568
65152
19.6%
2843
4.4%
58098
89.2%
4044
6.2 %
3097
4.8 %
1126
1.7 %
Parallel - persistent infection cull area
112
21742
6.5%
1038
4.8%
19604
90.2%
1435
6.6 %
1089
5.0 %
11
0.1 %
Parallel - Rapid Testing Two-times IRs
434
1512
0.5%
274
18.1%
1127
74.5%
96
6.3 %
72
4.8 %
39
2.6 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
3471 331966
100.0%
13314
4.0%
295738
89.1%
22682
6.8 %
17635
5.3 %
5314
1.6 %
England - HRA
Extended Flexible IFN-GAMMA test
29
1937
0.6%
103
5.3%
1722
88.9%
105
5.4 %
67
3.5 %
45
2.3 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
28
2134
0.6%
147
6.9%
1778
83.3%
265
12.4 %
209
9.8 %
0
Parallel - new infection cull area
602
71286
21.5%
3053
4.3%
62729
88.0%
5003
7.0 %
3888
5.5 %
1616
2.3 %
Parallel - Other
30
2238
0.7%
93
4.2%
1841
82.3%
316
14.1 %
262
11.7 %
42
1.9 %
Parallel Persistent Infection
168
27676
8.3%
1360
4.9%
24195
87.4%
1743
6.3 %
1331
4.8 %
791
2.9 %
Parallel - persistent infection cull area
111
21390
6.4%
1031
4.8%
19264
90.1%
1427
6.7 %
1084
5.1 %
11
0.1 %
Serial - Anomalous Reaction
3
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
England - Edge
Parallel New Infection
950 119706
36.1%
3764
3.1%
107421
89.7%
8235
6.9 %
6452
5.4 %
2087
1.7 %
Parallel - new infection cull area
10
4360
1.3%
114
2.6%
3992
91.6%
308
7.1 %
254
5.8 %
0
Parallel - Other
1
111
0.0%
7
6.3%
102
91.9%
3
2.7 %
2
1.8 %
0
Parallel Persistent Infection
19
2058
0.6%
57
2.8%
1899
92.3%
123
6.0 %
99
4.8 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
85
9840
3.0%
275
2.8%
8724
88.7%
977
9.9 %
803
8.2 %
38
0.4 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %
England
2054 263774
79.5%
10026
3.8%
234494
88.9%
18623
7.1 %
14547
5.5 %
4726
1.8 %
Wales - High TB
Extended Flexible IFN-GAMMA test
124
3279
1.0%
250
7.6%
2911
88.8%
151
4.6 %
115
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
146
8218
2.5%
469
5.7%
7365
89.6%
517
6.3 %
374
4.6 %
11
0.1 %
Parallel New Infection
38
1423
0.4%
81
5.7%
1284
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
48
2577
0.8%
175
6.8%
2270
88.1%
174
6.8 %
131
5.1 %
1
0.0 %
Parallel Persistent Infection
339
30398
9.2%
1274
4.2%
27465
90.4%
1851
6.1 %
1422
4.7 %
239
0.8 %
Parallel - Rapid Testing Two-times IRs
376
1224
0.4%
246
20.1%
876
71.6%
87
7.1 %
63
5.1 %
39
3.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
51
1216
0.4%
102
8.4%
996
81.9%
59
4.9 %
48
3.9 %
70
5.8 %
Parallel - Herd or Group Slaughter
7
110
0.0%
17
15.5%
80
72.7%
14
12.7 %
13
11.8 %
0
Parallel New Infection
108
10494
3.2%
312
3.0%
9512
90.6%
618
5.9 %
472
4.5 %
198
1.9 %
Parallel - Other
26
833
0.3%
56
6.7%
728
87.4%
58
7.0 %
49
5.9 %
0
Parallel Persistent Infection
32
3360
1.0%
136
4.0%
3097
92.2%
195
5.8 %
136
4.0 %
0
Parallel - Rapid Testing Two-times IRs
54
283
0.1%
28
9.9%
246
86.9%
9
3.2 %
9
3.2 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
35
1526
0.5%
40
2.6%
1397
91.5%
107
7.0 %
77
5.0 %
16
1.0 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.1%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1395
65499
19.7%
3198
4.9%
58755
89.7%
3932
6.0 %
2984
4.6 %
578
0.9 %
Scotland - Scotland
Parallel New Infection
20
2003
0.6%
81
4.0%
1820
90.9%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
22
2693
0.8%
90
3.3%
2489
92.4%
127
4.7 %
104
3.9 %
10
0.4 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| April        | 269     |
| May          | 364     |
| June         | 287     |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| Totals (last |         |
| 12 months)   |         |
| 3471         | 331966  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2054
263774
1303
10026
442
0
0
0
0
969
9
16
37
1
8434
7
82
0
1
Scotland
22
2693
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1395
65499
699
3198
145
0
0
0
0
124
7
8
16
1
2225
103
496
2
8
Totals
3471
331966
2013
13314
587
0
0
0
0
1105
16
24
55
2
10729
110
583
2
9

*Includes all animals with IFNg test negative, resample and reject outcomes

%Resampled %Retested
| Carms         | 2.45 %   | 3.10 %   |
|---------------|----------|----------|
| Ceredigion    | 1.17 %   | 2.34 %   |
| Cheshire      | 6.91 %   | 8.27 %   |
| Corn & Scilly | 3.84 %   | 4.70 %   |
| Derbyshire    | 3.59 %   | 4.51 %   |
| Devon         | 7.89 %   | 9.07 %   |
| Dorset        | 3.64 %   | 4.30 %   |
| E Sussex      | 2.14 %   | 5.00 %   |
| Gloucs        | 2.94 %   | 3.98 %   |
| Gr Manchester | 8.24 %   | 9.41 %   |
| Gwent         | 2.72 %   | 4.83 %   |
| Gwynedd       | 6.12 %   | 10.20 %  |
| Heref         | 6.52 %   | 7.77 %   |
| Leics & Rut   | 1.47 %   | 2.21 %   |
| NE Wales   | 4.08 %   | 5.99 %   |
|------------|----------|----------|
| Northants  | 5.18 %   | 6.20 %   |
| N Yorks    | 2.63 %   | 7.89 %   |
| Oxon       | 3.74 %   | 5.47 %   |
| Pembs      | 3.07 %   | 4.40 %   |
| Powys      | 6.16 %   | 8.05 %   |
| Somerset excl N   | 4.17 %   | 5.00 %   |
|-------------------|----------|----------|
| S Wales           |          |          |
| Warks             | 4.27 %   | 5.98 %   |
| W Glamorgan       | 5.66 %   | 15.09 %  |
| Wiltshire         | 3.15 %   | 3.97 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
% Positive samples
England
18769
623
3.3%
19595
623
3.2%
Wales
5491
281
5.1%
5597
281
5.0%
GB
24260
904
3.7%
25192
904
3.6%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
% Positive samples
England
15080
824
5.5%
15274
824
5.4%
Wales
6199
417
6.7%
6280
417
6.6%
GB
21279
1241
5.8%
21554
1241
5.8%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
% Positive samples
England
230030
10026
4.4%
263774
10026
3.8%
Wales
59269
3198
5.4%
65499
3198
4.9%
Scotland
2015
90
4.5%
2693
90
3.3%
GB
291304
13314
4.6%
331966
13314
4.0%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
% Positive samples
England
165901
9620
5.8%
193638
9620
5.0%
Wales
50414
2858
5.7%
57607
2869
5.0%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
218375
12659
5.8%
253486
12670
5.0%
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
2
0
0
Dorset
PRV-SP
2
6
0
0
Worcestershire
PRV-SE
1
16
0
0
Total
4
24
0
| % Positive                     | * Test type   |
|--------------------------------|---------------|
| Num                            |               |
| Submissions                    |               |
| Num                            |               |
| Samples                        |               |
| Num                            |               |
| Positives                      |               |
| Private                        |               |
| Devon                          | PRV-SE        |
| PRV-SP                         | 4             |
| Dorset                         | PRV-SP        |
| Gloucestershire                | PRV-SP        |
| Herefordshire                  | PRV-SP        |
| Northern Somerset & South Glou | PRV-SP        |
| Somerset excl North            | PRV-SE        |
| PRV-SP                         | 7             |
| Staffordshire                  | PRV-SE        |
| Wiltshire                      | PRV-SP        |
| Worcestershire                 | PRV-SE        |
| SER-OTHER                      | 1             |
| Total                          |               |
| 31                             |               |
| 377                            |               |
| 19                             | 5.04 %        |
| Isle of Man                    | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                       | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|-----------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077                | Carmarthenshire |        1 |                22 | 7                    | 31.8%                             |
| Cornwall and Isles of |                 |          |                   |                      |                                   |
| Scilly                |                 |          |                   |                      |                                   |
| 1                     | 26              |        0 |                 0 |                      |                                   |
| North East Wales      | 1               |       17 |                 2 | 11.8%                |                                   |
| Pembrokeshire         | 3               |       74 |                13 | 17.6%                |                                   |
| Powys                 | 3               |       60 |                 4 | 6.7%                 |                                   |
| TC0077                | 9               |      199 |                26 | 13.1 %               |                                   |
|                       | Test            | County   | Num Submissions   | Num Samples Tested   | Num Positive Samples % Positive   |
|-----------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077                | Carmarthenshire | 17       | 597               | 60                   | 10.1%                             |
| Ceredigion            | 3               | 372      | 17                | 4.6%                 |                                   |
| Cornwall and Isles of |                 |          |                   |                      |                                   |
| Scilly                |                 |          |                   |                      |                                   |
| 2                     | 263             | 30       | 11.4%             |                      |                                   |
| North East Wales      | 30              | 974      | 119               | 12.2%                |                                   |
| Pembrokeshire         | 24              | 841      | 78                | 9.3%                 |                                   |
| Powys                 | 12              | 440      | 31                | 7.0%                 |                                   |
| South Wales           | 5               | 196      | 16                | 8.2%                 |                                   |
| Wiltshire             | 1               | 248      | 9                 | 3.6%                 |                                   |
| TC0077                | 94              | 3,931    | 360               | 9.2 %                |                                   |