# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 162

# Report Period 1St - 28Th February 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| February                |                          |          |
| 2020                    |                          |          |
| Submissions             | 323                      | 666      |
| Samples                 | Total (%)                | 33146    |
| England                 | 27498                    | 82.96 %  |
| Wales                   | 5486                     | 16.55 %  |
| Scotland                | 162                      | 0.49 %   |
| Parallel Tests          | PAR-CUL-N                | 12527    |
| PAR-CUL-P               | 1029                     | 3.10 %   |
| PAR-HERD-S              | 204                      | 0.62 %   |
| PAR-NEW-IN              | 14045                    | 42.37 %  |
| PAR-OTHER               | 241                      | 0.73 %   |
| PAR-PERSIS              | 4440                     | 13.40 %  |
| PAR-RAPID               | 104                      | 0.31 %   |
| Total(% of all samples) | 32590                    | 98.32 %  |
| Serial/Extended Tests   | SER-FLEXI                | 556      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 556                      | 1.68 %   |
| Total                   | 33146                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1958     |
| England                 | 1585                     | 4.78 %   |
| Wales                   | 368                      | 1.11 %   |
| Scotland                | 5                        | 0.02 %   |
| Resamples               | Total (% of all samples) | 1571     |
| England                 | 1272                     | 3.84 %   |
| Wales                   | 294                      | 0.89 %   |
| Scotland                | 5                        | 0.02 %   |
| Rejects                 | Total (% of all samples) | 987      |
| England                 | 918                      | 2.77 %   |
| Wales                   | 4                        | 0.01 %   |
| Scotland                | 65                       | 0.20 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   12527 | 37.8 %   |
|-------------|---------|----------|
| PAR-CUL-P   |    1029 | 3.1 %    |
| PAR-HERD-S  |     204 | 0.6 %    |
| PAR-NEW-IN  |   14045 | 42.4 %   |
| PAR-OTHER   |     241 | 0.7 %    |
| PAR-PERSIS  |    4440 | 13.4 %   |
| PAR-RAPID   |     104 | 0.3 %    |
| SER-FLEXI   |     556 | 1.7 %    |
| Total       |   33146 | 100.00 % |
of Samples
submitted
by County

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For February 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-PERSIS      | 7              |
| Devon           | PAR-CUL-N      |
| SER-FLEXI       | 1              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 1              |
| SER-FLEXI       | 3              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| Shropshire      | PAR-PERSIS     |
| Somerset excl N | PAR-CUL-N      |
| Staffs          | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Gr Manchester   | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Lincs                   | PAR-NEW-IN     |
| N Yorks                 | PAR-NEW-IN     |
| Surrey                  | PAR-NEW-IN     |
| England                 |                |
| 193                     | 27498          |
| Wales - High TB         | Carms          |
| PAR-OTHER               | 7              |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 6              |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 2              |
| Gwent                   | PAR-PERSIS     |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 2              |
| Pembs                   | PAR-OTHER      |
| PAR-PERSIS              | 17             |
| PAR-RAPID               | 7              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 3              |
| PAR-OTHER               | 3              |
| PAR-PERSIS              | 8              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-PERSIS     |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Ceredigion          | PAR-PERSIS     |
| SER-FLEXI           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-PERSIS          | 3              |
| PAR-RAPID           | 3              |
| SER-FLEXI           | 1              |
| Powys               | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| S Glamorgan         | SER-FLEXI      |
| Wales - Low TB      | Gwynedd        |
| NE Wales            | PAR-NEW-IN     |
| Wales               |                |
| 129                 | 5486           |
| Scotland - Scotland | Argyll & Bute  |
| Scotland            |                |
| 1                   | 162            |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 15             |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 6              |
| SER-FLEXI       | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| PAR-NEW-IN      | 1              |
| SER-FLEXI       | 5              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-PERSIS     |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| Staffs          | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 2              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 6              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Gr Manchester   | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| Surrey          | PAR-NEW-IN     |
| England         |                |
| 409             | 51745          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 9              |
| PAR-PERSIS      | 14             |
| PAR-RAPID       | 13             |
| SER-FLEXI       | 1              |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 10             |
| PAR-RAPID       | 5              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 5              |
| SER-FLEXI       | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 18             |
| PAR-PERSIS              | 28             |
| PAR-RAPID               | 14             |
| SER-FLEXI               | 2              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 10             |
| PAR-OTHER               | 3              |
| PAR-PERSIS              | 16             |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 7              |
| S Wales                 | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| PAR-PERSIS              | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 2              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 6              |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 1              |
| Powys                   | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| S Glamorgan             | SER-FLEXI      |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| Wales               |                |
| 246                 | 11243          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 11                  | 1432           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 5            |
| Total           | 193          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 1            |
| Wales           | Parallel     |
| Serial/Extended | 9            |
| Total           | 129          |
| GB              | Parallel     |
| Serial/Extended | 14           |
| Total           | 323          |
| 193.00          |              |
| 129.00          |              |
| 1.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 13           |
| Total           | 409          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 11           |
| Wales           | Parallel     |
| Serial/Extended | 19           |
| Total           | 246          |
| GB              | Parallel     |
| Serial/Extended | 32           |
| Total           | 666          |
| 409.00          |              |
| 246.00          |              |
| 11.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (February 2020)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
14
556
1.7%
49
8.8%
496
89.2%
22
4.0 %
11
2.0 %
0
Parallel - Herd or Group Slaughter
5
204
0.6%
18
8.8%
180
88.2%
13
6.4 %
6
2.9 %
0
Parallel New Infection
101
14045
42.4%
368
2.6%
12111
86.2%
879
6.3 %
748
5.3 %
818
5.8 %
Parallel - new infection cull area
91
12527
37.8%
428
3.4%
11389
90.9%
702
5.6 %
545
4.4 %
165
1.3 %
Parallel - Other
23
241
0.7%
40
16.6%
173
71.8%
32
13.3 %
28
11.6 %
0
Parallel Persistent Infection
57
4440
13.4%
234
5.3%
4004
90.2%
254
5.7 %
199
4.5 %
3
0.1 %
Parallel - persistent infection cull area
4
1029
3.1%
131
12.7%
866
84.2%
53
5.2 %
31
3.0 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
28
104
0.3%
21
20.2%
80
76.9%
3
2.9 %
3
2.9 %
0
Total
323
33146
100.0%
1289
3.9%
29299
88.4%
1958
5.9 %
1571
4.7 %
987
3.0 %
England - HRA
Extended Flexible IFN-GAMMA test
5
370
1.1%
20
5.4%
343
92.7%
13
3.5 %
7
1.9 %
0
Parallel New Infection
2
165
0.5%
5
3.0%
153
92.7%
11
6.7 %
7
4.2 %
0
Parallel - new infection cull area
91
12527
37.8%
428
3.4%
11389
90.9%
702
5.6 %
545
4.4 %
165
1.3 %
Parallel Persistent Infection
10
2080
6.3%
85
4.1%
1900
91.3%
118
5.7 %
94
4.5 %
1
0.0 %
Parallel - persistent infection cull area
4
1029
3.1%
131
12.7%
866
84.2%
53
5.2 %
31
3.0 %
1
0.1 %
England - HRA
112
16171
48.8%
669
4.1%
14651
90.6%
897
5.5 %
684
4.2 %
167
1.0 %
England - Edge
Parallel New Infection
75
10703
32.3%
293
2.7%
9296
86.9%
672
6.3 %
573
5.4 %
541
5.1 %
England - Edge
75
10703
32.3%
293
2.7%
9296
86.9%
672
6.3 %
573
5.4 %
541
5.1 %
England - LRA
Parallel New Infection
6
624
1.9%
11
1.8%
388
62.2%
16
2.6 %
15
2.4 %
210
33.7 %
England - LRA
6
624
1.9%
11
1.8%
388
62.2%
16
2.6 %
15
2.4 %
210
33.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
6
106
0.3%
18
17.0%
86
81.1%
7
6.6 %
2
1.9 %
0
Parallel - Herd or Group Slaughter
5
204
0.6%
18
8.8%
180
88.2%
13
6.4 %
6
2.9 %
0
Parallel New Infection
3
400
1.2%
20
5.0%
348
87.0%
35
8.8 %
30
7.5 %
2
0.5 %
Parallel - Other
22
229
0.7%
40
17.5%
163
71.2%
29
12.7 %
26
11.4 %
0
Parallel Persistent Infection
42
2197
6.6%
125
5.7%
1975
89.9%
126
5.7 %
95
4.3 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
24
96
0.3%
17
17.7%
76
79.2%
3
3.1 %
3
3.1 %
0
Wales - High TB
102
3232
9.8%
238
7.4%
2828
87.5%
213
6.6 %
162
5.0 %
4
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
80
0.2%
11
13.8%
67
83.8%
2
2.5 %
2
2.5 %
0
Parallel New Infection
10
1447
4.4%
29
2.0%
1343
92.8%
91
6.3 %
75
5.2 %
0
Parallel - Other
1
12
0.0%
0
0.0 %
10
83.3%
3
25.0 %
2
16.7 %
0
Parallel Persistent Infection
5
163
0.5%
24
14.7%
129
79.1%
10
6.1 %
10
6.1 %
0
Parallel - Rapid Testing Two-times IRs
4
8
0.0%
4
50.0%
4
50.0%
0
0
0
Wales - Intermediate TB
23
1710
5.2%
68
4.0%
1553
90.8%
106
6.2 %
89
5.2 %
0
Wales - Low TB
Parallel New Infection
4
544
1.6%
9
1.7%
492
90.4%
49
9.0 %
43
7.9 %
0
Wales - Low TB
4
544
1.6%
9
1.7%
492
90.4%
49
9.0 %
43
7.9 %
0
Scotland - Scotland
Parallel New Infection
1
162
0.5%
1
0.6%
91
56.2%
5
3.1 %
5
3.1 %
65
40.1 %
Scotland - Scotland
1
162
0.5%
1
0.6%
91
56.2%
5
3.1 %
5
3.1 %
65
40.1 %
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
32
1656
2.6%
107
6.5%
1495
90.3%
80
4.8 %
54
3.3 %
0
Parallel - Herd or Group Slaughter
11
226
0.4%
20
8.8%
199
88.1%
16
7.1 %
7
3.1 %
0
Parallel New Infection
228
27667
42.9%
771
2.8%
24282
87.8%
1776
6.4 %
1441
5.2 %
1173
4.2 %
Parallel - new infection cull area
175
21288
33.0%
829
3.9%
19137
89.9%
1301
6.1 %
1022
4.8 %
300
1.4 %
Parallel - Other
36
837
1.3%
89
10.6%
697
83.3%
63
7.5 %
51
6.1 %
0
Parallel Persistent Infection
115
9508
14.8%
559
5.9%
8473
89.1%
593
6.2 %
466
4.9 %
10
0.1 %
Parallel - persistent infection cull area
17
3073
4.8%
241
7.8%
2695
87.7%
192
6.2 %
136
4.4 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
52
165
0.3%
40
24.2%
120
72.7%
5
3.0 %
5
3.0 %
0
Total
666
64420
100.0%
2656
4.1%
57098
88.6%
4026
6.2 %
3182
4.9 %
1484
2.3 %
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.9%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
4
409
0.6%
15
3.7%
357
87.3%
48
11.7 %
36
8.8 %
1
0.2 %
Parallel - new infection cull area
174
20927
32.5%
814
3.9%
18810
89.9%
1262
6.0 %
1003
4.8 %
300
1.4 %
Parallel Persistent Infection
24
4546
7.1%
207
4.6%
4144
91.2%
256
5.6 %
192
4.2 %
3
0.1 %
Parallel - persistent infection cull area
17
3073
4.8%
241
7.8%
2695
87.7%
192
6.2 %
136
4.4 %
1
0.0 %
England - Edge
Parallel New Infection
166
20374
31.6%
606
3.0%
17985
88.3%
1251
6.1 %
997
4.9 %
786
3.9 %
Parallel - new infection cull area
1
361
0.6%
15
4.2%
327
90.6%
39
10.8 %
19
5.3 %
0
England - LRA
Parallel New Infection
10
831
1.3%
14
1.7%
481
57.9%
24
2.9 %
22
2.6 %
314
37.8 %
England
409
51745
80.3%
1982
3.8%
45912
88.7%
3131
6.1 %
2446
4.7 %
1405
2.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
14
348
0.5%
25
7.2%
312
89.7%
19
5.5 %
11
3.2 %
0
Parallel - Herd or Group Slaughter
11
226
0.4%
20
8.8%
199
88.1%
16
7.1 %
7
3.1 %
0
Parallel New Infection
10
1273
2.0%
50
3.9%
1083
85.1%
148
11.6 %
133
10.4 %
7
0.5 %
Parallel - Other
34
824
1.3%
89
10.8%
686
83.3%
60
7.3 %
49
5.9 %
0
Parallel Persistent Infection
80
4610
7.2%
297
6.4%
4048
87.8%
321
7.0 %
258
5.6 %
7
0.2 %
Parallel - Rapid Testing Two-times IRs
44
153
0.2%
35
22.9%
113
73.9%
5
3.3 %
5
3.3 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
84
0.1%
12
14.3%
70
83.3%
2
2.4 %
2
2.4 %
0
Parallel New Infection
21
2433
3.8%
50
2.1%
2252
92.6%
157
6.5 %
131
5.4 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel - Other
2
13
0.0%
0
0.0 %
11
84.6%
3
23.1 %
2
15.4 %
0
Parallel Persistent Infection
11
352
0.5%
55
15.6%
281
79.8%
16
4.5 %
16
4.5 %
0
Parallel - Rapid Testing Two-times IRs
7
11
0.0%
5
45.5%
6
54.5%
0
0
0
Wales - Low TB
Parallel New Infection
6
915
1.4%
12
1.3%
848
92.7%
69
7.5 %
55
6.0 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
246
11243
17.5%
650
5.8%
9910
88.1%
816
7.3 %
669
6.0 %
14
0.1 %
Scotland - Scotland
Parallel New Infection
11
1432
2.2%
24
1.7%
1276
89.1%
79
5.5 %
67
4.7 %
65
4.5 %
Scotland
11
1432
2.2%
24
1.7%
1276
89.1%
79
5.5 %
67
4.7 %
65
4.5 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | March   |
| April        | 269     |
| May          | 364     |
| June         | 287     |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| 2020         | January |
| February     | 323     |
| Totals (last |         |
| 12 months)   |         |
| 3528         | 340829  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2113
272219
1319
9648
552
0
0
0
0
1019
7
7
42
1
7899
8
64
0
1
Scotland
33
4125
17
114
2
0
0
0
0
13
0
1
2
0
90
0
5
0
0
Wales
1382
64485
701
3018
164
0
0
0
0
126
5
7
19
3
2073
99
501
0
15
Totals
3528
340829
2037
12780
718
0
0
0
0
1158
12
15
63
4
10062
107
570
0
16

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Argyll & Bute | 3.09 %   | 3.09 %                 |
| Berks         | 8.70 %   |                        |
| Carms         | 4.31 %   | 5.77 %                 |
| Ceredigion    | 5.81 %   | 5.81 %                 |
| Cheshire      | 4.70 %   | 5.47 %                 |
| Corn & Scilly | 3.88 %   | 4.98 %                 |
| Cumbria       | 3.33 %   | 6.67 %                 |
| Derbyshire    | 7.25 %   | 8.06 %                 |
| Devon         | 4.74 %   | 5.92 %                 |
| Dorset        | 3.18 %   | 4.55 %                 |
| E Sussex      | 3.10 %   | 3.98 %                 |
| Gloucs        | 7.61 %   | 9.04 %                 |
| Gr Manchester |          |                        |
| Gwent         | 2.02 %   | 7.07 %                 |
| Gwynedd       | 11.11 %  | 11.11 %                |
| Hampshire     | 5.51 %   | 5.51 %                 |
| Heref         | 4.86 %   | 7.60 %                 |
| Leics & Rut   | 3.33 %   | 3.33 %                 |
| Lincs         | 8.62 %   | 8.62 %                 |
| NE Wales      | 6.10 %   | 7.14 %                 |
| N Yorks       |          |                        |
| Oxon          | 3.58 %   | 5.27 %                 |
| Pembs         | 4.90 %   | 6.05 %                 |
| Powys           | 6.25 %   | 7.57 %   |
|-----------------|----------|----------|
| S Glamorgan     |          |          |
| Shropshire      | 5.25 %   | 6.27 %   |
| Somerset excl N | 2.93 %   | 4.08 %   |
| Staffs          | 5.61 %   | 6.60 %   |
| Surrey          | 3.56 %   | 3.56 %   |
| Warks           | 5.78 %   | 6.74 %   |
| W Glamorgan     | 16.67 %  | 16.67 %  |
| Wiltshire       | 3.91 %   | 5.73 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
26941
973
3.6%
27498
973
3.5%
Wales
5430
315
5.8%
5486
315
5.7%
Scotland
162
1
0.6%
162
1
0.6%
GB
32533
1289
4.0%
33146
1289
3.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
22388
1471
6.6%
22715
1471
6.5%
Wales
5155
436
8.5%
5329
436
8.2%
GB
27543
1907
6.9%
28044
1907
6.8%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
49708
1982
4.0%
51745
1982
3.8%
Wales
10872
650
6.0%
11243
650
5.8%
Scotland
1387
24
1.7%
1432
24
1.7%
GB
61967
2656
4.3%
64420
2656
4.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
41359
2360
5.7%
43300
2360
5.5%
Wales
11725
830
7.1%
12257
830
6.8%
GB
53084
3190
6.0%
55557
3190
5.7%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Cheshire
PRV-SP
1
1
0
0
Devon
PRV-SE
1
119
0
0
Dorset
PRV-SP
1
3
0
0
Gloucestershire
PRV-SE
1
8
3
37.50 %
Oxfordshire
PRV-SP
1
32
0
0
Total
5
163
3
1.84 %
Isle of Man
SER-IOM
2
32
0
0
| % Positive      | * Test type   |
|-----------------|---------------|
| Num             |               |
| Submissions     |               |
| Num             |               |
| Samples         |               |
| Num             |               |
| Positives       |               |
| Private         |               |
| Cheshire        | PRV-SP        |
| Devon           | PRV-SE        |
| PRV-SP          | 1             |
| Dorset          | PRV-SE        |
| PRV-SP          | 1             |
| Gloucestershire | PRV-SE        |
| Oxfordshire     | PRV-SP        |
| Staffordshire   | PRV-SE        |
| Worcestershire  | PRV-SP        |
| Total           |               |
| 9               |               |
| 195             |               |
| 3               | 1.54 %        |
| Isle of Man     | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        5 |               166 | 24                   | 14.5%                             |
| Ceredigion       | 1               |        2 |                 0 | 0                    |                                   |
| Devon            | 4               |      402 |                53 | 13.2%                |                                   |
| North East Wales | 3               |       92 |                12 | 13.0%                |                                   |
| Pembrokeshire    | 6               |      169 |                24 | 14.2%                |                                   |
| South Wales      | 3               |       10 |                 1 | 10.0%                |                                   |
| TC0077           | 22              |      841 |               114 | 13.6 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 6        |               183 | 26                   | 14.2%                             |
| Ceredigion       | 1               | 2        |                 0 | 0                    |                                   |
| Devon            | 4               | 1,032    |                95 | 9.2%                 |                                   |
| North East Wales | 6               | 135      |                18 | 13.3%                |                                   |
| Pembrokeshire    | 9               | 237      |                33 | 13.9%                |                                   |
| South Wales      | 4               | 45       |                 4 | 8.9%                 |                                   |
| TC0077           | 30              | 1,634    |               176 | 10.8 %               |                                   |