# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 157

# Report Period 1St - 30Th September 2019 Issued 10 October 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| September               |                          |          |
| 2019                    |                          |          |
| Submissions             | 245                      | 2667     |
| Samples                 | Total (%)                | 25317    |
| England                 | 20801                    | 82.16 %  |
| Wales                   | 4509                     | 17.81 %  |
| Scotland                | 7                        | 0.03 %   |
| Parallel Tests          | PAR-CUL-N                | 7695     |
| PAR-CUL-P               | 1983                     | 7.83 %   |
| PAR-HERD-S              | 371                      | 1.47 %   |
| PAR-NEW-IN              | 10590                    | 41.83 %  |
| PAR-OTHER               | 154                      | 0.61 %   |
| PAR-PERSIS              | 4220                     | 16.67 %  |
| PAR-RAPID               | 63                       | 0.25 %   |
| Total(% of all samples) | 25076                    | 99.05 %  |
| Serial/Extended Tests   | SER-FLEXI                | 241      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 241                      | 0.95 %   |
| Total                   | 25317                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1972     |
| England                 | 1739                     | 6.87 %   |
| Wales                   | 230                      | 0.91 %   |
| Scotland                | 3                        | 0.01 %   |
| Resamples               | Total (% of all samples) | 1456     |
| England                 | 1282                     | 5.06 %   |
| Wales                   | 171                      | 0.68 %   |
| Scotland                | 3                        | 0.01 %   |
| Rejects                 | Total (% of all samples) | 5        |
| England                 | 4                        | 0.02 %   |
| Wales                   | 1                        | 0.00 %   |
| Scotland                | 0                        | 0.00 %   |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   7695 | 30.4 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   1983 | 7.8 %    |
| PAR-HERD-S  |    371 | 1.5 %    |
| PAR-NEW-IN  |  10590 | 41.8 %   |
| PAR-OTHER   |    154 | 0.6 %    |
| PAR-PERSIS  |   4220 | 16.7 %   |
| PAR-RAPID   |     63 | 0.2 %    |
| SER-FLEXI   |    241 | 1.0 %    |
| Total       |  25317 | 100.00 % |
Breakdown
of Samples
submitted
by County

Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For September 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| Dorset          | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| Gloucestershire | PAR-CUL-N      |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Heref           | PAR-PERSIS     |
| Shropshire      | PAR-OTHER      |
| Somerset excl N | PAR-CUL-N      |
| Staffs          | PAR-OTHER      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 3              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Berks          |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 25             |
| Derbyshire      | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2A.  Summary By County For September 2019 Cont.

| Country                 | County          |
|-------------------------|-----------------|
| Submission              |                 |
| Reasons*                |                 |
| No.                     |                 |
| subs                    |                 |
| Samples                 | Gamma Positive  |
| n                       | % of total      |
| Leics & Rut             | PAR-NEW-IN      |
| Oxon                    | PAR-NEW-IN      |
| Warks                   | PAR-NEW-IN      |
| England - LRA           | Cumbria         |
| Gr London               | PAR-NEW-IN      |
| Lincs                   | PAR-NEW-IN      |
| N Yorks                 | PAR-PERSIS      |
| England                 |                 |
| 147                     | 20801           |
| Wales - High TB         | Carmarthenshire |
| Carms                   | PAR-HERD-S      |
| PAR-OTHER               | 1               |
| PAR-PERSIS              | 5               |
| PAR-RAPID               | 2               |
| SER-FLEXI               | 2               |
| Ceredigion              | PAR-PERSIS      |
| PAR-RAPID               | 5               |
| SER-FLEXI               | 3               |
| Pembrokeshire           | PAR-RAPID       |
| Pembs                   | PAR-HERD-S      |
| PAR-OTHER               | 1               |
| PAR-PERSIS              | 14              |
| PAR-RAPID               | 9               |
| SER-FLEXI               | 3               |
| Powys                   | PAR-HERD-S      |
| PAR-NEW-IN              | 3               |
| PAR-PERSIS              | 3               |
| PAR-RAPID               | 3               |
| Wales - Intermediate TB | NE Wales        |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2A.  Summary By County For September 2019 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-OTHER           | 4              |
| PAR-PERSIS          | 5              |
| PAR-RAPID           | 1              |
| SER-FLEXI           | 7              |
| Wales - Low TB      | Gwynedd        |
| NE Wales            | PAR-NEW-IN     |
| PAR-RAPID           | 1              |
| Wales               |                |
| 97                  | 4509           |
| Scotland - Scotland | Kirkcudbright  |
| Scotland            |                |
| 1                   | 7              |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 9              |
| SER-FRAUD       | 2              |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 7              |
| PAR-PERSIS      | 31             |
| SER-FLEXI       | 2              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 27             |
| PAR-NEW-IN      | 4              |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 19             |
| SER-FLEXI       | 8              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 13             |
| PAR-NEW-IN      | 3              |
| SER-FLEXI       | 4              |
| Gloucestershire | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 10             |
| PAR-HERD-S      | 4              |
| SER-FLEXI       | 2              |
| Heref           | PAR-CUL-N      |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-CUL-P       | 2              |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 29             |
| Shropshire      | PAR-CUL-P      |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 6              |
| PAR-PERSIS      | 10             |
| SER-FLEXI       | 5              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 13             |
| Staffs          | PAR-OTHER      |
| PAR-PERSIS      | 7              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 19             |
| PAR-NEW-IN      | 7              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 9              |
| SER-FLEXI       | 1              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 185            |
| PAR-PERSIS      | 5              |
| Derbyshire      | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country            | County         |
|--------------------|----------------|
| Submission         |                |
| Reasons*           |                |
| No.                |                |
| subs               |                |
| Samples            | Gamma Positive |
| n                  | % of total     |
| E Sussex           | PAR-NEW-IN     |
| Hampshire          | PAR-NEW-IN     |
| PAR-PERSIS         | 1              |
| SER-NSR            | 2              |
| Leicestershire and |                |
| Rutland            |                |
| PAR-NEW-IN         | 1              |
| Leics & Rut        | PAR-NEW-IN     |
| Northants          | PAR-NEW-IN     |
| Notts              | PAR-NEW-IN     |
| Oxon               | PAR-NEW-IN     |
| Warks              | PAR-NEW-IN     |
| PAR-PERSIS         | 6              |
| England - LRA      | Cambs          |
| Cumbria            | PAR-NEW-IN     |
| Essex              | PAR-NEW-IN     |
| Gr London          | PAR-NEW-IN     |
| Gr Manchester      | PAR-NEW-IN     |
| Herts              | PAR-NEW-IN     |
| I of Wight         | PAR-NEW-IN     |
| Kent               | PAR-NEW-IN     |
| Lancashire         | PAR-NEW-IN     |
| Lincs              | PAR-NEW-IN     |
| Northumb           | PAR-NEW-IN     |
| PAR-PERSIS         | 3              |
| N Yorks            | PAR-NEW-IN     |
| PAR-PERSIS         | 3              |
| SER-FLEXI          | 2              |
| S Yorks            | PAR-NEW-IN     |
| W Sussex           | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County          |
|-----------------|-----------------|
| Submission      |                 |
| Reasons*        |                 |
| No.             |                 |
| subs            |                 |
| Samples         | Gamma Positive  |
| n               | % of total      |
| W Yorks         | PAR-HERD-S      |
| England         |                 |
| 1578            | 200794          |
| Wales - High TB | Carmarthenshire |
| Carms           | PAR-HERD-S      |
| PAR-NEW-IN      | 3               |
| PAR-OTHER       | 14              |
| PAR-PERSIS      | 64              |
| PAR-RAPID       | 61              |
| SER-FLEXI       | 37              |
| Ceredigion      | PAR-HERD-S      |
| PAR-NEW-IN      | 2               |
| PAR-OTHER       | 3               |
| PAR-PERSIS      | 27              |
| PAR-RAPID       | 40              |
| SER-FLEXI       | 8               |
| Gwent           | PAR-HERD-S      |
| PAR-OTHER       | 1               |
| PAR-PERSIS      | 16              |
| PAR-RAPID       | 24              |
| SER-FLEXI       | 1               |
| Pembrokeshire   | PAR-RAPID       |
| Pembs           | PAR-HERD-S      |
| PAR-NEW-IN      | 3               |
| PAR-OTHER       | 15              |
| PAR-PERSIS      | 119             |
| PAR-RAPID       | 101             |
| SER-FLEXI       | 41              |
| Powys           | PAR-HERD-S      |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-NEW-IN              | 24             |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 32             |
| PAR-RAPID               | 54             |
| SER-FLEXI               | 11             |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 8              |
| PAR-PERSIS              | 8              |
| PAR-RAPID               | 13             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| SER-FLEXI           | 38             |
| Powys               | PAR-NEW-IN     |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 3              |
| S Wales             | PAR-RAPID      |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 2              |
| PAR-RAPID           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 2              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1073                | 46887          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 16                  | 2217           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol September 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 147          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 1            |
| Wales           | Parallel     |
| Serial/Extended | 15           |
| Total           | 97           |
| GB              | Parallel     |
| Serial/Extended | 15           |
| Total           | 245          |
| 147.00          |              |
| 97.00           |              |
| 1.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 28           |
| Total           | 1578 200794  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 16           |
| Wales           | Parallel     |
| Serial/Extended | 147          |
| Total           | 1073         |
| GB              | Parallel     |
| Serial/Extended | 175          |
| Total           | 2667 249898  |

####### #######
16.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (September 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
241
1.0%
9
3.7%
224
92.9%
12
5.0 %
8
3.3 %
0
Parallel - Herd or Group Slaughter
11
371
1.5%
14
3.8%
343
92.5%
24
6.5 %
14
3.8 %
0
Parallel New Infection
80
10590
41.8%
177
1.7%
9776
92.3%
851
8.0 %
635
6.0 %
2
0.0 %
Parallel - new infection cull area
51
7695
30.4%
236
3.1%
6905
89.7%
743
9.7 %
552
7.2 %
2
0.0 %
Parallel - Other
10
154
0.6%
6
3.9%
138
89.6%
19
12.3 %
10
6.5 %
0
Parallel Persistent Infection
44
4220
16.7%
192
4.5%
3871
91.7%
217
5.1 %
165
3.9 %
1
0.0 %
Parallel - persistent infection cull area
11
1983
7.8%
50
2.5%
1861
93.8%
106
5.3 %
72
3.6 %
0
Parallel - Rapid Testing Two-times IRs
23
63
0.2%
9
14.3%
54
85.7%
0
0
0
Total
245
25317
100.0%
693
2.7%
23172
91.5%
1972
7.8 %
1456
5.8 %
5
0.0 %
England - HRA
Parallel New Infection
5
445
1.8%
3
0.7%
418
93.9%
34
7.6 %
24
5.4 %
0
Parallel - new infection cull area
50
7657
30.2%
234
3.1%
6876
89.8%
734
9.6 %
545
7.1 %
2
0.0 %
Parallel - Other
4
119
0.5%
5
4.2%
108
90.8%
14
11.8 %
6
5.0 %
0
Parallel Persistent Infection
10
1300
5.1%
118
9.1%
1136
87.4%
69
5.3 %
46
3.5 %
0
Parallel - persistent infection cull area
11
1983
7.8%
50
2.5%
1861
93.8%
106
5.3 %
72
3.6 %
0
England - HRA
80
11504
45.4%
410
3.6%
10399
90.4%
957
8.3 %
693
6.0 %
2
0.0 %
England - Edge
Parallel New Infection
59
8795
34.7%
145
1.6%
8151
92.7%
671
7.6 %
497
5.7 %
2
0.0 %
Parallel - new infection cull area
1
38
0.2%
2
5.3%
29
76.3%
9
23.7 %
7
18.4 %
0
Parallel Persistent Infection
1
37
0.1%
2
5.4%
28
75.7%
9
24.3 %
7
18.9 %
0
England - Edge
61
8870
35.0%
149
1.7%
8208
92.5%
689
7.8 %
511
5.8 %
2
0.0 %
England - LRA
Parallel New Infection
5
405
1.6%
4
1.0%
323
79.8%
93
23.0 %
78
19.3 %
0
Parallel Persistent Infection
1
22
0.1%
0
0.0 %
22
100.0%
0
0
0
England - LRA
6
427
1.7%
4
0.9%
345
80.8%
93
21.8 %
78
18.3 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
8
83
0.3%
2
2.4%
78
94.0%
5
6.0 %
3
3.6 %
0
Parallel - Herd or Group Slaughter
11
371
1.5%
14
3.8%
343
92.5%
24
6.5 %
14
3.8 %
0
Parallel New Infection
3
12
0.0%
0
0.0 %
12
100.0%
0
0
0
Parallel - Other
2
15
0.1%
1
6.7%
12
80.0%
2
13.3 %
2
13.3 %
0
Parallel Persistent Infection
27
1845
7.3%
58
3.1%
1712
92.8%
94
5.1 %
74
4.0 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
21
59
0.2%
9
15.3%
50
84.7%
0
0
0
Wales - High TB
72
2385
9.4%
84
3.5%
2207
92.5%
125
5.2 %
93
3.9 %
1
0.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
158
0.6%
7
4.4%
146
92.4%
7
4.4 %
5
3.2 %
0
Parallel New Infection
4
787
3.1%
18
2.3%
744
94.5%
38
4.8 %
25
3.2 %
0
Parallel - Other
4
20
0.1%
0
0.0 %
18
90.0%
3
15.0 %
2
10.0 %
0
Parallel Persistent Infection
5
1016
4.0%
14
1.4%
973
95.8%
45
4.4 %
38
3.7 %
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Intermediate TB
21
1983
7.8%
39
2.0%
1883
95.0%
93
4.7 %
70
3.5 %
0
Wales - Low TB
Parallel New Infection
3
139
0.5%
7
5.0%
124
89.2%
12
8.6 %
8
5.8 %
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Low TB
4
141
0.6%
7
5.0%
126
89.4%
12
8.5 %
8
5.7 %
0
Scotland - Scotland
Parallel New Infection
1
7
0.0%
0
0.0 %
4
57.1%
3
42.9 %
3
42.9 %
0
Scotland - Scotland
1
7
0.0%
0
0.0 %
4
57.1%
3
42.9 %
3
42.9 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
171
5643
2.3%
376
6.7%
4957
87.8%
288
5.1 %
209
3.7 %
101
1.8 %
Parallel - Herd or Group Slaughter
130
4806
1.9%
366
7.6%
4149
86.3%
369
7.7 %
284
5.9 %
8
0.2 %
Parallel New Infection
979 114073
45.6%
3805
3.3%
102803
90.1%
8528
7.5 %
6689
5.9 %
794
0.7 %
Parallel - new infection cull area
464
54789
21.9%
2619
4.8%
48727
88.9%
3983
7.3 %
3077
5.6 %
366
0.7 %
Parallel - Other
79
4113
1.6%
263
6.4%
3421
83.2%
476
11.6 %
387
9.4 %
42
1.0 %
Parallel Persistent Infection
433
49544
19.8%
2138
4.3%
44394
89.6%
3094
6.2 %
2381
4.8 %
643
1.3 %
Parallel - persistent infection cull area
79
15955
6.4%
828
5.2%
14260
89.4%
1113
7.0 %
860
5.4 %
7
0.0 %
Parallel - Rapid Testing Two-times IRs
328
949
0.4%
192
20.2%
687
72.4%
61
6.4 %
43
4.5 %
27
2.8 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
2667 249898
100.0%
10587
4.2%
223423
89.4%
17913
7.2 %
13931
5.6 %
1988
0.8 %
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.6%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
24
2088
0.8%
139
6.7%
1743
83.5%
262
12.5 %
206
9.9 %
0
Parallel - new infection cull area
462
54491
21.8%
2598
4.8%
48469
88.9%
3962
7.3 %
3058
5.6 %
366
0.7 %
Parallel - Other
24
1661
0.7%
88
5.3%
1280
77.1%
298
17.9 %
251
15.1 %
42
2.5 %
Parallel Persistent Infection
128
21043
8.4%
1068
5.1%
18404
87.5%
1389
6.6 %
1044
5.0 %
528
2.5 %
Parallel - persistent infection cull area
79
15955
6.4%
828
5.2%
14260
89.4%
1113
7.0 %
860
5.4 %
7
0.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
733
92840
37.2%
3026
3.3%
83846
90.3%
6784
7.3 %
5299
5.7 %
687
0.7 %
Parallel - new infection cull area
2
298
0.1%
21
7.0%
258
86.6%
21
7.0 %
19
6.4 %
0
Parallel Persistent Infection
15
1351
0.5%
29
2.1%
1262
93.4%
75
5.6 %
60
4.4 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
71
8570
3.4%
261
3.0%
7575
88.4%
851
9.9 %
696
8.1 %
38
0.4 %
Parallel Persistent Infection
6
758
0.3%
3
0.4%
583
76.9%
89
11.7 %
79
10.4 %
93
12.3 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

England
1578 200794
80.4%
8149
4.1%
179220
89.3%
14948
7.4 %
11638
5.8 %
1806
0.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
101
2809
1.1%
196
7.0%
2512
89.4%
128
4.6 %
98
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
118
4648
1.9%
341
7.3%
4032
86.7%
351
7.6 %
268
5.8 %
8
0.2 %
Parallel New Infection
32
1021
0.4%
56
5.5%
917
89.8%
55
5.4 %
47
4.6 %
1
0.1 %
Parallel - Other
35
1855
0.7%
123
6.6%
1631
87.9%
135
7.3 %
101
5.4 %
0
Parallel Persistent Infection
262
23028
9.2%
941
4.1%
20980
91.1%
1387
6.0 %
1087
4.7 %
22
0.1 %
Parallel - Rapid Testing Two-times IRs
286
832
0.3%
173
20.8%
592
71.2%
58
7.0 %
40
4.8 %
27
3.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
44
1089
0.4%
96
8.8%
893
82.0%
58
5.3 %
47
4.3 %
53
4.9 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
79
6805
2.7%
217
3.2%
6204
91.2%
433
6.4 %
332
4.9 %
52
0.8 %
Parallel - Other
18
579
0.2%
51
8.8%
495
85.5%
40
6.9 %
33
5.7 %
0
Parallel Persistent Infection
19
2597
1.0%
88
3.4%
2427
93.5%
130
5.0 %
91
3.5 %
0
Parallel - Rapid Testing Two-times IRs
39
113
0.0%
19
16.8%
91
80.5%
3
2.7 %
3
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
25
870
0.3%
25
2.9%
798
91.7%
47
5.4 %
31
3.6 %
16
1.8 %
Parallel - Other
2
18
0.0%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
3
4
0.0%
0
0.0 %
4
100.0%
0
0
0
Wales
1073
46887
18.8%
2355
5.0%
42154
89.9%
2862
6.1 %
2208
4.7 %
182
0.4 %
Scotland - Scotland
Parallel New Infection
15
1879
0.8%
81
4.3%
1720
91.5%
96
5.1 %
78
4.2 %
0
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland
16
2217
0.9%
83
3.7%
2049
92.4%
103
4.6 %
85
3.8 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | October |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| April        | 269     |
| May          | 364     |
| June         | 287     |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| Totals (last |         |
| 12 months)   |         |
| 3451         | 319998  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2016
253686
1319
11105
296
0
0
0
0
983
13
3
43
5
9619
8
96
0
2
Scotland
18
2232
10
83
0
0
0
0
0
12
0
0
2
0
63
1
4
0
0
Wales
1417
64080
714
3637
93
0
0
0
0
166
7
3
16
1
2613
74
620
2
23
Totals
3451
319998
2043
14825
389
0
0
0
0
1161
20
6
61
6
12295
83
720
2
25

*Includes all animals with IFNg test negative, resample and reject outcomes

|                 | County   | %Resampled %Retested   |
|-----------------|----------|------------------------|
| Avon            | 50.00 %  | 50.00 %                |
| Berks           | 5.56 %   | 6.11 %                 |
| Carmarthenshire |          |                        |
| Carms           | 3.30 %   | 4.58 %                 |
| Ceredigion      | 5.79 %   | 7.63 %                 |
| Cheshire        | 5.11 %   | 7.13 %                 |
| Corn & Scilly   | 5.86 %   | 7.73 %                 |
| Cumbria         | 6.02 %   | 18.07 %                |
| Derbyshire      | 7.51 %   | 9.74 %                 |
| Devon           | 5.71 %   | 7.43 %                 |
| Dorset          | 6.61 %   | 8.89 %                 |
| Gloucestershire |          |                        |
| Gloucs          | 9.39 %   | 11.93 %                |
| Gr London       | 50.00 %  | 100.00 %               |
| Gwynedd         | 5.80 %   | 8.70 %                 |
| Hampshire       | 10.86 %  | 13.74 %                |
| Heref           | 7.27 %   | 9.09 %                 |
| Kirkcudbright   | 42.86 %  | 42.86 %                |
| Leics & Rut     | 25.00 %  |                        |
| Lincs           | 22.50 %  | 23.75 %                |
| NE Wales        | 3.52 %   | 4.68 %                 |
| N Yorks         |          |                        |
| Oxon            | 6.51 %   | 8.65 %                 |
| Pembrokeshire   |          |                        |
| Pembs           | 3.91 %   | 5.05 %   |
|-----------------|----------|----------|
| Powys           | 3.09 %   | 4.54 %   |
| Shropshire      | 4.76 %   | 11.43 %  |
| Somerset excl N | 5.16 %   | 10.24 %  |
| Staffs          | 8.33 %   |          |
| Warks           | 4.33 %   | 5.23 %   |
| Wiltshire       | 6.31 %   | 8.08 %   |
| Worcs           |          |          |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
20426
563
2.8%
20801
563
2.7%
Wales
4458
130
2.9%
4509
130
2.9%
Scotland
7
0
0.0 %
7
0.0 %
GB
24891
693
2.8%
25317
693
2.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
16078
665
4.1%
16273
665
4.1%
Wales
5072
281
5.5%
5168
281
5.4%
GB
21148
946
4.5%
21441
946
4.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
181696
8149
4.5%
200794
8149
4.1%
Wales
42681
2355
5.5%
46887
2355
5.0%
Scotland
1889
83
4.4%
2217
83
3.7%
GB
226262
10587
4.7%
249898
10587
4.2%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
123818
6664
5.4%
140746
6664
4.7%
Wales
35833
1587
4.4%
40414
1587
3.9%
Scotland
2066
181
8.8%
2226
181
8.1%
GB
161700
8432
5.2%
183386
8432
4.6%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Dorset
PRV-SP
1
2
0
0
Somerset excl North
PRV-SE
2
206
1
0.49 %
Staffordshire
PRV-SE
1
64
3
4.69 %
Total
4
272
4
1.47 %
Isle of Man
SER-IOM
5
248
1
0.40 %
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Devon               | PRV-SE        |
| PRV-SP              | 1             |
| Dorset              | PRV-SP        |
| Gloucestershire     | PRV-SP        |
| Herefordshire       | PRV-SP        |
| Somerset excl North | PRV-SE        |
| PRV-SP              | 3             |
| Staffordshire       | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 17                  |               |
| 337                 |               |
| 19                  | 5.64 %        |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        1 |                79 | 5                    | 6.3%                              |
| North East Wales | 5               |      113 |                 9 | 8.0%                 |                                   |
| Pembrokeshire    | 2               |       81 |                 5 | 6.2%                 |                                   |
| TC0077           | Sum:            |      273 |                19 | 7.0 %                |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 13       |               506 | 39                   | 7.7%                              |
| Ceredigion       | 3               | 372      |                17 | 4.6%                 |                                   |
| North East Wales | 24              | 850      |               112 | 13.2%                |                                   |
| Pembrokeshire    | 16              | 546      |                32 | 5.9%                 |                                   |
| Powys            | 6               | 313      |                18 | 5.8%                 |                                   |
| South Wales      | 5               | 196      |                16 | 8.2%                 |                                   |
| Wiltshire        | 1               | 248      |                 9 | 3.6%                 |                                   |
| TC0077           | Sum:            | 3,031    |               243 | 8.0 %                |                                   |