# North-West Of Jones Bank Rmcz Post-Survey Site Report

 
Contract Reference: MB0120 
Report Number: 19 
Version 6 
August 2015 
 
 
Project Title: Marine Protected Areas Data and Evidnece Co-ordination Programme Report No: 19. Title: North-West of Jones Bank rMCZ Post-survey Site Report Defra Project Code: MB0120 Defra Contract Manager: Carole Kelly Funded by: Department for Environment, Food and Rural Affairs (Defra) Marine Science and Evidence Unit Marine Directorate Nobel House 17 Smith Square London SW1P 3JR 
Authorship Dr Alice Jones NERC Marine Geosciences Group, National Oceanography Centre, Southampton alice.jones@noc.ac.uk Dr Tim Le Bas NERC Marine Geosciences Group, National Oceanography Centre, Southampton tlb@noc.ac.uk Dr Russell Wynn NERC Marine Geosciences Group, National Oceanography Centre, Southampton Rbw1@noc.ac.uk Acknowledgements We thank Andrew Colenutt (CCO) for reviewing earlier drafts of this report and Gareth Grewcock (CCO) for advice regarding GIS mapping. Disclaimer:  The content of this report does not necessarily reflect the views of Defra, nor is Defra liable for the accuracy of information provided, or responsible for any use of the reports content.  Although the data provided in this report has been quality assured, the final products - e.g. habitat maps - may be subject to revision following any further data provision or once they have been used in SNCB advice or assessments.  

## Cefas Document Control Title: North-West Of Jones Bank Rmcz Post-Survey Site Report

Submitted to: 
rMCZ Project Steering Group 
Date submitted: 
February 2015 
Project Manager: 
David Limpenny 
Report compiled by: 
Dr Alice Jones, Dr Tim Le Bas and Dr Russell Wynn (NOCS) 
Quality control by: 
Keith Weston 
Approved by & date: 
Christopher Barrio (14/08/2015) 
Version: 
6 

## 

Version Control History Author 
Date 
Comment 
Version 
Dr Alice Jones 
17th July 2013 
First draft 
1 
19th July 2013 
Amended draft 
2 
Dr Tim Le Bas & Dr Russell Wynn Dr Alice Jones 
7th August 2013 
Revised draft following Cefas QC 
3 
Dr Alice Jones 
23rd January 2014 
Revised draft following Cefas QC 
4 
Dr Sue Ware 
3rd February 2015 
Revised draft following MPAG review 
5 
Dr Chris Barrio 
14th August 2015 
Final revisions addressed 
6 

## 

Annexes ................................................................................................................... 26 
Annex 1.  Broadscale habitat features listed in the ENG. ..................................... 26 Annex 2.  Habitat FOCI listed in the ENG. 
............................................................ 27 
Annex 3.  Low or limited mobility species FOCI listed in the ENG. 
....................... 28 
Annex 4.  Highly mobile species FOCI listed in the ENG. 
..................................... 29 
Annex 5.  Video and stills processing protocol. .................................................... 30 
Appendices .............................................................................................................. 32 
Appendix 1. Survey metadata (Gardline cruise (Job #9060) and CEND10/12)) ... 32 Appendix 2. Outputs from acoustic surveys 
.......................................................... 37 
Appendix 3. Evidence of human activities within the rMCZ .................................. 41 
Appendix 4. Species list 
........................................................................................ 42 
Appendix 5. Analyses of sediment samples: classification and composition ........ 48 Appendix 6. BSH/EUNIS Level 3 descriptions derived from video and stills 
......... 50 
Appendix 7.  Example images from survey of broadscale habitats 
....................... 52 
Appendix 8. Example images from survey of habitat FOCI .................................. 53 

## List Of Tables

Table 1.  Broadscale habitats for which this rMCZ was proposed for designation. .... 4 Table 2.  Habitat FOCI for which this rMCZ was proposed for designation. ............... 5 
Table 3.  Species FOCI for which this rMCZ was proposed for designation. 
.............. 5 
Table 4.  Description of derivatives calculated from bathymetry data. ..................... 11 Table 5.  Broadscale habitats identified in this rMCZ. .............................................. 16 Table 6.  Habitat FOCI identified in this rMCZ. 
......................................................... 18 
Table 7.  Species FOCI identified in this rMCZ. ....................................................... 18 
 

## List Of Figures

Figure 1.  Location of the North-West of Jones Bank rMCZ.  Bathymetry is from the 

GEBCO Digital Atlas (published by the British Oceanographic Data Centre on 
behalf of IOC and IHO, 2003). ........................................................................... 4 

Figure 2.  Location of ground truth sampling sites in the North-West of Jones Bank 

rMCZ.  Bathymetry is from the GEBCO Digital Atlas (published by the British Oceanographic Data Centre on behalf of IOC and IHO, 2003). 
......................... 8 

Figure 3.  Habitat map from the Site Assessment Document (SAD).  The hashed 'No 

data area' refers to the parts of the site where no new data were collected 
during the 2012 surveys. 
.................................................................................. 12 

Figure 4.  Updated map of broadscale habitats based on newly acquired survey data 

| (grey shading indicates areas where underlying MBES data are absent).    |    14 |
|--------------------------------------------------------------------------|-------|
|                                                                          |       |
| Figure 5.  Overall MESH confidence score for the updated habitat map.    |    15 |
|                                                                          |       |
| Figure 6.  Habitat FOCI identified in the updated habitat map.           |    17 |
|                                                                          |       |
|                                                                          |       |

## 1 Executive Summary: Report Card

This report details the findings of a dedicated seabed survey at the North-West of Jones Bank recommended Marine Conservation Zone (rMCZ).  The site is being considered for inclusion in a network of Marine Protected Areas (MPAs) in UK 
waters, designed to meet conservation objectives under the Marine and Coastal Access Act 2009.  Prior to the dedicated survey, the site assessment had been made on the basis of 'best available evidence', drawn largely from historical data, modelled habitat maps and stakeholder knowledge of the area.  The purpose of the survey was to provide direct evidence of the presence and extent of the broadscale habitats (BSH) and habitat FOCI (Features of Conservation Importance) that had been detailed in the original Site Assessment Document (SAD) (Lieberknecht et al, 2011). 

This Executive Summary is presented in the form of a Report Card comparing the characteristics predicted in the original SAD with the updated habitat map and new sample data that result from the two surveys of the site conducted by Gardline in March 2012 (Job #9060) and Cefas in July 2012 (CEND10/12).  The comparison covers broadscale habitats (BSH) and habitat and species FOCI. 

## 1.1 Features Proposed In The Sad For Inclusion Within The Mcz Designation

| Extent in SAD                 | Extent          | Accordance between    |
|-------------------------------|-----------------|-----------------------|
| sub-area                      | according to    | sub-area of SAD map   |
| Extent                        |                 |                       |
| according to                  | subject to 2012 | updated               |
| Feature                       | SAD (2011)      | survey                |
| Broadscale Habitats (BSH)     |                 |                       |
| A5.1 Subtidal coarse sediment | 3.75 km         |                       |
| 2                             |                 |                       |
|                               | 3.75 km         |                       |
| 2                             |                 |                       |
|                               | 13.66 km        |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                              |                 |                       |
|                               | +9.91 km        |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                               |                 |                       |
| A5.2 Subtidal sand            | 5.90 km         |                       |
| 2                             |                 |                       |
|                               |                 |                       |
| 5.90 km                       |                 |                       |
| 2**                           |                 |                       |
|                               |                 |                       |
| 36.85 km                      |                 |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                              |                 |                       |
|                               | +30.95 km       |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                               |                 |                       |
| A5.3 Subtidal mud             | 388.45 km       |                       |
| 2                             |                 |                       |
|                               | 295.43 km       |                       |
| 2                             |                 |                       |
|                               | 253.34 km       |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                              |                 |                       |
|                               | - 42.09 km      |                       |
| 2                             |                 |                       |
|                               |                 |                       |
| Habitat FOCI                  |                 |                       |
| Subtidal Sands and Gravels    |                 |                       |
| *                             |                 |                       |
|                               | 9.65 km         |                       |
| 2                             |                 |                       |
|                               | 9.65 km         |                       |
| 2                             |                 |                       |
|                               | 50.51 km        |                       |
| 2                             |                 |                       |
|                               |                 |                       |
|                              |                 |                       |
|                               | +40.86 km       |                       |
| 2                             |                 |                       |
|                               |                 |                       |
| Species FOCI                  |                 |                       |
| None proposed                 | N/A             | N/A                   |

* The extent of this habitat FOCI has been re-calculated by summing the extent estimates of the 'A5.1 Subtidal coarse sediment' and 'A5.2 Subtidal sand' BSHs in the SAD. N.B. Spatial extents for all BSH and Habitat FOCI have been adjusted to compare the updated habitat map extents with the sub-area of the SAD covering the same extent as that covered by the newly acquired data. 

## 1.2 Features Present But Not Proposed In The Sad For Inclusion Within The Rmcz Designation

| Extent                                       | Extent       |
|----------------------------------------------|--------------|
| according                                    | according to |
| to SAD                                       | updated      |
| Accordance between SAD                       |              |
| Feature                                      | (2011)       |
| Broadscale Habitats (BSH)                    |              |
| A5.4 Subtidal mixed sediments                | 0 km         |
| 2                                            |              |
|                                              | 1.15 km      |
| 2                                            |              |
|                                              |              |
|                                             |              |
|                                              | +1.15 km     |
| 2                                            |              |
|                                              |              |
| Habitat FOCI                                 |              |
|                                              |              |
|                                              |              |
|                                              |              |
| Mud Habitats in Deepwater                    | 0 km         |
| 2                                            |              |
|                                              | 253.34 km    |
| 2                                            |              |
|                                              |              |
|                                             |              |
|                                              | +253.34 km   |
| 2                                            |              |
|                                              |              |
| Sea-Pen and Burrowing Megafauna Communities* | 0 km         |
| 2                                            |              |
|                                              | Unknown      |
|                                             |              |
|                                              | Unknown      |
| Species FOCI                                 |              |
|                                              |              |
| None                                         | N/A          |

 
NB. Spatial extents for all BSH and Habitat FOCI have been adjusted to compare the updated habitat map extents with the sub-area of the SAD covering the same extent as that covered by the newly acquired data. 

*The presence of this Habitat FOCI is known from the video and still images, but the extent of its coverage has not been estimated. 

 

## 1.3 Evidence Of Human Activities Occurring Within The Rmcz

There is evidence from the multibeam backscatter image that demersal trawling occurs within this rMCZ.  Numerous trawl marks, left by bottom-towed fishing gear, can be clearly identified within the boundary of the rMCZ (Appendix 3). 

## 2 Introduction

In accordance with the Marine and Coastal Access Act 2009, the UK is committed to the development and implementation of a network of Marine Protected Areas (MPAs).  The network will incorporate existing designated sites (e.g., Special Areas of Conservation and Special Protection Areas) along with a number of newly designated sites which, within the English territorial waters and offshore waters of England, Wales and Northern Ireland, will be termed Marine Conservation Zones (MCZs).  In support of this initiative, four Regional MCZ Projects were set up to select sites that could contribute to this network because they contain one or more features specified in the Ecological Network Guidance (ENG; Natural England and the JNCC, 2010).  The Regional MCZ Projects proposed a total of 127 recommended MCZs (rMCZs) and compiled a Site Assessment Document (SAD) for each site, summarising what evidence was available for the presence and extent of the various habitat, species and geological features specified in the ENG and for which the site was being recommended. Due to the scarcity of survey-derived seabed habitat maps in UK waters, these assessments were necessarily made using 'best available evidence', which included historical data, modelled habitat maps and stakeholder knowledge of the areas concerned. It became apparent that the 'best available evidence' on features for which some sites had been recommended as MCZs was of variable quality.  Consequently, Defra initiated a number of measures aimed at improving the evidence base, one of which took the form of a dedicated survey programme, implemented and co-ordinated by Cefas, to collect and interpret new survey data at selected rMCZ sites.  This report provides an interpretation of the survey data collected jointly by Gardline, Cefas and JNCC personnel at the North-West of Jones Bank rMCZ site during two cruises which took place in March and July 2012. 

## 2.1 Location Of The Rmcz

The North-West of Jones Bank rMCZ is a square block, approximately 400 km2 located at the boundary between the Western Channel and Celtic Sea, approximately 180 km due west from Land's End at the southwest tip of Cornwall (Figure 1). 

 

## 2.2 Rationale For Site Position And Designation

The North-West of Jones Bank rMCZ was included in the proposed network because of its contribution to Ecological Network Guidance (ENG) criteria to broadscale habitats, and its added ecological importance.  For a detailed site description see 'Finding Sanctuary Final Report and Recommendations, 2011' (Lieberknecht et al., 
2011, section II.3.4) and 'The Marine Conservation Zone Project:  Ecological Network Guidance' (Natural England and the JNCC, 2010). 

## 2.2.1 Broadscale Habitats Proposed For Designation

Three broadscale habitats were included in the recommendations for designation at this site (Table 1).  See Annex 1 for a full list of broadscale habitat features listed in the ENG. 

| EUNIS code & Broadscale Habitat    | Spatial extent according to the SAD    |
|------------------------------------|----------------------------------------|
| A5.1 Subtidal coarse sediment      | 3.75 km                                |
| 2                                  |                                        |
|                                    |                                        |
| A5.2 Subtidal sand                 | 5.90 km                                |
| 2                                  |                                        |
|                                    |                                        |
| A5.3 Subtidal mud                  | 388.45 km                              |
| 2                                  |                                        |
|                                    |                                        |

## 2.2.2 Habitat Foci Proposed For Designation

Annex 2 presents the habitat FOCI listed in the ENG.  The habitat FOCI 'Subtidal sands and gravels' was included in the recommendations for designation at this site (Table 2).  The estimated spatial extent for this habitat FOCI in the SAD was calculated using seabed sediment data layers from the British Geological Survey DigSBS250 GIS product (for further information see Seeley et al, 2010), rather than by combining the extent of the UK SeaMap 'subtidal coarse sediment' and 'subtidal sand' broadscale habitats given in Table 1. 

| Habitat FOCI               | Spatial extent according to SAD    |
|----------------------------|------------------------------------|
| Subtidal Sands and Gravels | 9.65 km                            |
| 2                          |                                    |
| *                          |                                    |

* The extent of this Habitat FOCI has been re-calculated by summing the extent estimates of the 'A5.1 Subtidal coarse sediment' and 'A5.2 Subtidal sand' BSH, in accordance with guidance from the JNCC and NE. 

## 2.2.3 Species Foci Proposed For Designation

No 'Low or limited mobility species' and no 'Highly mobile species' FOCI were included in the recommendations for designation of this rMCZ (Table 3).  The full list of these species FOCI is presented in Annexes 3 and 4. 

| Species FOCI                         |      |
|--------------------------------------|------|
| Low or limited mobility species FOCI | None |
| Highly mobile species FOCI           | None |

## 2.3 Rationale For Prioritising This Rmcz For Additional Evidence Collection

Prioritisation of rMCZ sites for further evidence collection was informed by a gap analysis and evidence assessment.  The prime objective was to elevate the confidence status for as many rMCZs as feasible to support designation in terms of the amount and quality of evidence for the presence and extent of broadscale habitat features and habitat and species FOCI.  The confidence status was originally assessed in the SADs according Technical Protocol E (Natural England and the JNCC, 2012). The confidence score for the presence and extent of broadscale habitats and habitat FOCI reported for the North-West of Jones Bank rMCZ was 'Low/Moderate' (JNCC and Natural England, 2012).  This site was therefore prioritised for additional evidence collection. 

## 2.4 Survey Aims And Objectives Primary Objectives

 To collect acoustic and ground-truthing data to allow the production of an 
updated map which could be used to inform the presence of broadscale 
habitats and habitat FOCI, and allow estimates to be made of their spatial extent within the rMCZ. 

## Secondary Objectives

 To provide evidence, where possible, of the presence of species FOCI listed 
within the ENG (Annexes 3 and 4) within the rMCZ. 
 To report evidence of human activity occurring within the rMCZ during the 
course of the survey. 
It should be emphasised that surveys were not primarily designed to address the secondary objectives under the current programme of work. 

Whilst the newly collected data will be utilised for the purposes of reporting against the primary objectives of the current programme of work (given above), it is recognised that these data will be valuable for informing the assessment and monitoring of condition of given habitat features in the future. 

## 3 Methods 3.1 Acoustic Data Acquisition

Multibeam bathymetry and backscatter data were used to assist in the planning and interpretation of seabed habitats.  The bathymetric data were collected and processed in accordance with the International Hydrographic Organisation (IHO) Standards for Hydrographic Surveys - Order 1 (Special Publication 44, Edition 4). Two datasets were used: 1) The main multibeam bathymetry and backscatter dataset for the site was collected between 29th February and 31st March 2012 on a survey carried out by Gardline aboard the MV *Tridens* (9060).  This dataset covered approximately 75 % of the proposed rMCZ site.  These data were collected using a hull-mounted Simrad EM710 echo sounder (calibrated by a patch test at the start of the survey). The acoustic data were processed using Caris HIPS and SIPS (version 7.1).  Tidal information was loaded to the relevant files and the survey lines merged.  The merging process combines all sensor data along with tidal and offset information to produce updated soundings files, prior to depth editing.  Depth data were processed using the Swathe Editor function, where each line is cleaned manually and statistically for noise.  The dataset is gridded to create a Bathymetry Associated with Statistical Error (BASE) Surface with different child layers to enable further processing and checking using the Subset function.  A BASE surface is a georeferenced image of a multi-attributed, weighted mean surface.  The gridding technique used is a combination of range and swathe angle weighting.  The range weight is inversely proportional to the distance from the node (the position of the node is determined during the creation of the fieldsheet): soundings closer to the node have more weighting.  The swathe angle weighting ensures that higher value is given to beams from the inner part of the swathe than to outer beams from adjacent lines.  The surface is created using a single resolution of 2 m x 2 m, which is determined by several factors including system, water depth, line spacing and data quality. Velocity profiles were undertaken using a Valeport 650 MKII MIDAS velocity probe whilst the vessel was static and, when required, during data acquisition whilst vessel speed was reduced to 4 knots. Backscatter data were also processed using Caris HIPS and SIPS (version 7.1) software.  There is no ability to make gain changes on the backscatter data during acquisition on the EM710 and during processing, gains were not altered either in order to maintain uniform gain across the whole site.  There was some breakup of the backscatter data due to the marginal weather that the survey was conducted in at times, but it was agreed that the data were fit for purpose and met the requirements of Cefas and so acquisition continued. 2) A further survey was carried out by Cefas and JNCC on the RV *Cefas Endeavour* between 11th and 12th July 2012.  The aim of this cruise was to collect additional benthic samples from within the rMCZ area, although some lines of multibeam acoustic data were also recorded whilst transiting between sampling stations.  These data cover approximately 6 % of the rMCZ site.  The raw multibeam bathymetry data were processed using Caris HIPS.  Tidal information was gathered using a CNAV 3050 DGPS receiver.  Tidal height data were smoothed and extracted to reduce the effects of tidal height on the bathymetry data.  The soundings were cleaned and smoothed, using Caris, to IHO order 1.  Multibeam backscatter data were processed with Fledermaus Geocoder Toolbox (FMGT) to produce standard and floating point (FP) geotiffs. See Appendix 2 for images derived from both sets of acoustic data. 

## 3.2 Ground-Truth Sample Acquisition

Ground-truth samples were collected on both the Gardline and Cefas survey cruises from 50 stations in total, which were positioned within the sedimentary habitats using a triangular lattice grid overlaid on the Site Assessment Document (SAD) habitat map.  Benthic grabs were taken at 44 stations to collect sediments and infauna.  An underwater camera system was deployed at 17 stations to collect video and still images of the seabed (Figure 2; Appendix 1). 


## Grab Sample Acquisition:

The survey carried out by Gardline (9060) in March 2012 used two instruments to collect a total of 36 grab samples from the seafloor.  These were an in-house constructed, modified stainless-steel 0.1 m2 Day Grab, and a 0.1 m2 mini Hamon grab.  Eight additional grab samples were collected on the Cefas cruise 
(CEND10/12) in July 2012, using a 0.1 m2 mini Hamon grab fitted with a video camera (this combined gear is known as a 'HamCam').  This allowed an image of the undisturbed seabed to be obtained before each grab sample. Grab samples deemed acceptable for analyses were photographed and described prior to sub-sampling.  A sub-sample of sediment was taken (either using a 3 cm diameter corer for samples acquired using the Day grab, or approx. 0.5 litres from Hamon grab samples) and placed into a labelled plastic container for later Particle Size Analysis (PSA).  The volume of the remaining sample was measured and recorded, then sieved over a 1 mm mesh to collect the benthic fauna.  The retained faunal samples were preserved in a known concentration (less than 20 %) of 'formalin' for later processing ashore. 

## Video And Still Image Sample Acquisition:

Within the broadscale habitat areas identified in the SAD, camera sledge deployments were made during both surveys at a subset of stations sampled by the grab, and also at 6 additional stations where grab samples were not collected successfully.  The camera images helped to characterise the surficial sediments and associated epifaunal communities.  The total number of camera deployments for each BSH varied depending on the uniformity of the habitat and their spatial extent. The methods used on the two surveys are outlined below: 1) Gardline survey (9060), March 2012.  Still images of the seabed were collected by means of a digital stills camera system with dedicated strobe and video lamps, mounted within a stainless steel frame.  Standard definition (SD) video footage was collected at all stations where images were collected.  At some stations, additional footage was acquired using a high definition (HD) camera.  An Ultra Short Baseline (USBL) positioning beacon was attached to the camera frame.  SD footage was viewed in real time via an umbilical, assisting in the control of the digital stills camera.  This allowed for shot selection, in the event that the system recorded a sediment change or feature of interest at the seafloor. A minimum of fourteen seabed photographs were taken at each station using a drift transect technique, separated by a gap time of approximately 1 minute.  This technique allowed the frame to move progressively along the seabed as the vessel traversed the work area on its thrusters or drifted.  The vessel would position 600 m in-line with the target and then drift over the station.  Recording was commenced when the camera position was approximately 150 m from target location.  Images were captured remotely using the surface control unit and stored on the camera's internal memory card.  Video footage was overlaid with time, position, depth and recorded directly onto VHS video and DVD and DVC tape.  On completion, photographs were downloaded onto a PC, via a USB download cable, and copied onto CD-Rom.  All CDs, DVDs, DVC tapes and videos were labelled with the relevant job details, write-protected and stored.  Footage was downloaded from the HD camera and stored on an expansion drive. 2) Cefas survey (CEND 10/12), July 2012.  Video observations were made with a camera sledge system, having a video camera with capability to also capture still images.  Illumination was provided by two Cefas high intensity LED striplights and a dedicated flash unit.  The camera was oriented to provide a forward oblique view of the seabed and was fitted with a four-spot (red) laser-scaling device which projected the corners of a 17 cm x 17 cm square along the axis of the lens onto the seabed, in order to provide a reference scale in the video.  A further (green) horizontal laser helped to visualise the rugosity of the seabed on the moving video image (but was not always clearly visible in the still images). 

Set-up and operation followed the MESH 'Recommended Operating Guidelines (ROG) for underwater video and photographic imaging techniques' (Coggan et al., 2007).  Video was recorded simultaneously to a Sony GV-HD700 DV tape and a computer hard drive.  A video overlay was used to provide station metadata, time and GPS position (of the vessel) in the recorded video image. Camera sledge transects lasted a minimum of 10 minutes, with the sledge being towed at c. 0.5 knots (c. 0.25 m s-1) along the desired transect line.  Still images were captured at regular one minute intervals and also opportunistically if specific features of interest were encountered.  The sledge was controlled by a winch operator with sight of the video monitor and note made of the amount of tow cable deployed to allow a 'lay-back' calculation to be applied to estimate the position of the sledge. For further detail on ground-truth sample collection see 'The North-West of Jones Bank rMCZ Survey Report' (Coggan, 2012) and 'Lot 6 - Northwest Jones Bank DEFRA MCZ programme 2012 Acquisition Report, March 2012' (Gardline, 2012). Video and still images were analysed following an established protocol developed and used by Cefas (Coggan and Howell, 2005; JNCC, in prep.; see Annex 5). 

## 3.3 Production Of The Updated Habitat Map

All new maps and their derivatives have been based on a WGS84 datum.  A new habitat map for the site was produced by analysing and interpreting the available acoustic data and the ground-truth data collected by the dedicated surveys of this site. The mapping process involved the production of derived datasets from the bathymetry data using tools in the ArcGIS v.10 Spatial Analyst Toolbox (slope, aspect and hillshade) and the DEM Surface Tools add-in (surface area/rugosity), as detailed in Table 4.  These data layers, and the processed depth and backscatter data layers, were used to create a broadscale habitat map of the rMCZ area through expert visual interpretation.  The absence of a full coverage acoustic data set across the entire site precluded the use of automated approaches in the interpretation carried out to produce the updated habitat map. Areas exhibiting different characteristics in the acoustic data were identified and digitised in ArcGIS v.10.  Sediment type was then assigned to each delineated area of the seabed using information from the ground-truthing data (both from sediment PSA and visual classification based on video and still images).  Where there was an absence of acoustic data the broadscale habitat map has been manually interpolated using expert judgement. 

| Derivative                                                             |
|------------------------------------------------------------------------|
| Slope                                                                  |
| The slope (gradient in degrees) for each cell of the bathymetry grid   |
| using the maximum change in elevation of each cell and its 8           |
| neighbours (Burrough and McDonnell, 1998).                             |
| Roughness (surface area                                                |
| ratio)                                                                 |
| A measure of topographic roughness.  Calculated by dividing the        |
| surface area of a cell and its 8 neighbours by the planimetric area of |
| these 9 cells (Jenness, 2004).                                         |
| Aspect                                                                 |
| Identifies the downslope direction of the maximum rate of change in    |
| value from each cell and its 8 neighbours.  Can be thought of as the   |
| slope direction.  Measured in clockwise degrees from 0 (due north).    |
| Flat areas with no downslope are given a value of -1 (Burrough and     |
| McDonnell, 1998).                                                      |
| Hillshade                                                              |
| Creates a shaded relief from a bathymetry surface by considering the   |
| illumination source angle and shadows.  Creating a hillshade layer     |
| greatly enhances the visualisation of bathymetry (depth) data          |
| (Burrough and McDonnell, 1998).                                        |

## 3.4 Quality Of The Updated Map

The technical quality of the updated habitat map was assessed using the MESH 'Confidence Assessment' Tool1, originally developed by an international consortium of marine scientists working on the MESH (Mapping European Seabed Habitats) project.  This tool considers the provenance of the data used to make a biotope/habitat map, including the techniques and technology used to characterise the physical and biological environment and the expertise of the people who had made the map.  In its original implementation it was used to make an auditable judgement of the confidence that could be placed in a range of existing, local biotope maps that had been developed using different techniques and data inputs, but were to be used in compiling a full coverage map for north-west Europe.  Where two of the original maps overlapped, that with the highest MESH confidence score would take precedence in the compiled map. Subsequent to the MESH project, the confidence assessment tool has been applied to provide a benchmark score that reflects the technical quality of newly developed habitat/biotope maps.  Both physical and biological survey data are required to achieve the top mark of 100, but as the current rMCZ exercise requires the mapping of broadscale physical habitats, not biotopes, it excludes the need for biological data. In the absence of biological data, the maximum score attainable for a physical habitat map is 88. In applying the tool to the current work, none of the weighting options were altered; that is, the tool was applied in its standard form, as downloaded from the internet. 

## 4 Results 4.1 Site Assessment Document (Sad) Habitat Map

The SAD habitat map (Figure 3) was produced using modelled data from the UKSeaMap (McBreen, 2010).  For further detail see Lieberknecht et al. (2011). 

 

## 4.2 Updated Habitat Map Based On New Survey Data

The updated habitat map, resulting from an integrated analysis of the data collected in the 2012 dedicated surveys, is presented in Figure 4.  There was an area of ~90 km2 where no acoustic data were collected.  Where the acoustic data gaps occur, the broadscale habitat map has been manually interpolated and, as Figure 4 illustrates, presents the interpolated areas of habitat in a separate colour within the map and legend. The estimated extent of the broadscale habitats and habitat FOCI from the SAD were re-calculated for the sub-area of the site that was covered by the new survey data.  This has enabled 'like-for-like' comparison of the spatial extent of each BSH/FOCI in the original SAD and in the site report for this rMCZ. The list of benthic taxa found in the grab and video samples is presented in Appendix 4; a total of 159 infaunal taxa were recorded from the grab samples and 191 epifaunal taxa were recorded from the video and still image data. 

A summary of the sediment PSA derived from the grab samples is given in Appendix 5.  Of the 44 stations where a sample was obtained, muddy sediment was recorded at 34 stations, sandy sediment at five stations, coarse sediment at two stations, and mixed sediments at three stations. 

The analysis of the seabed video and stills is summarised in Appendix 6.  Example still images of the BSHs and habitat FOCI recorded at this rMCZ during the survey are given in Appendices 7 and 8 respectively. 

 
North-West of Jones Bank rMCZ Post-survey Site 

## 4.3 Quality Of The Updated Habitat Map

The results of the MESH confidence assessment are shown in Figure 5.  Both the acoustic data collected on the Gardline survey (9060 in March 2012) and on the Cefas survey (CEND 10/12 in July 2012) received a score of 84 from the MESH 
Confidence Assessment Tool.  These scores are out of a possible 88 for a purely physical habitat map. There are gaps in the acoustic data in the western part of the site.  Approximately 23% of the site is not covered by the acoustic datasets.  The 'no data areas' have been attributed a confidence score of 0 and are represented as interpolated habitats in Figure 4. 

 

## 4.4 Broadscale Habitats Identified

Broadscale habitats were interpreted and mapped for the 77% of the area of the rMCZ site for which acoustic data were available.  The habitat map produced for the remaining area, where acoustic data were absent, was based on manual interpolation using expert judgement. 'A5.3 Subtidal mud' was the most widespread broadscale habitat type, occupying approximately two thirds of the rMCZ site (Figure 4, Table 5) and was to be found to be most prevalent in the deeper sections of the site, in particular in the central and western parts. 

'A5.2 Subtidal sand' was estimated to occupy 9.3% of the site and is present in clear longitudinal features, associated with shallower depths and often topped with 'A5.1 Subtidal coarse sediment' habitats, which occupied approximately 3.4% of the total area.  'A5.4 Subtidal mixed sediments' was also present, in association with the sand and coarse sediment features, but its extent was estimated at just 0.3% of the area of the site. 

The habitat map produced for an area of 92.83 km2, in the western side of the rMCZ, was based on interpolation of data due to the absence of acoustic data within these regions of the site (Figure 4 and Figure 5).  This interpolated section constitutes 23% of the full area of the rMCZ. 

|                               | Spatial extent      | Spatial extent    |
|-------------------------------|---------------------|-------------------|
| according to the              | within the sub-     | Spatial extent    |
| SAD for the entire            | according to the    |                   |
| Broadscale Habitat Type       |                     |                   |
| (EUNIS Level 3)               | rMCZ.               |                   |
| area of new                   |                     |                   |
| survey data                   | updated habitat map |                   |
| A5.1 Subtidal coarse sediment | 3.75 km             |                   |
| 2                             |                     |                   |
|                               | 3.75 km             |                   |
| 2                             |                     |                   |
|                               | 13.66 km            |                   |
| 2                             |                     |                   |
|                               |                     |                   |
| A5.2 Subtidal sand            | 5.90 km             |                   |
| 2                             |                     |                   |
|                               | 5.90 km             |                   |
| 2                             |                     |                   |
|                               | 36.85 km            |                   |
| 2                             |                     |                   |
|                               |                     |                   |
| A5.3 Subtidal mud             | 388.45 km           |                   |
| 2                             |                     |                   |
|                               | 295.43 km           |                   |
| 2                             |                     |                   |
|                               | 253.34 km           |                   |
| 2                             |                     |                   |
|                               |                     |                   |
| A5.4 Subtidal mixed sediments | 0 km                |                   |
| 2                             |                     |                   |
|                               | 0 km                |                   |
| 2                             |                     |                   |
|                               | 1.15 km             |                   |
| 2                             |                     |                   |
|                               |                     |                   |

## 4.5 Habitat Foci Identified

The habitat map presented in the SAD estimated that 328.44 km2 (> 80%) of the North-West of Jones Bank rMCZ was covered by the habitat FOCI 'Subtidal Sands and Gravels'.  This estimate was derived from MB102 (2C) data (Lieberknecht et al., 2011), largely based on the BGS DigSBS250 modelled seabed sediment data layers.  However, this estimate does not appear to agree with the UKSeaMap modelled broadscale habitat classification for the site, or the updated broadscale habitat map based on the new data collected at the site, which shows that much of the area is made up of muddy sediment (Figure 4).  This discrepancy is likely to be due to the filters used when extracting data from DigSBS250, which are based on Folk sediment type classifications.  Seeley et al. (2010) state that the Folk types 
'sand', 'gravel', 'sandy gravel', 'gravelly sand', 'muddy sand', 'slightly gravelly sand' 
and 'slightly gravelly muddy sand' were all used to feed into the 'Subtidal Sands and Gravels' habitat layer produced for the MB102 (2C).  The inclusion of some sediment types from the 'mud' end of the spectrum in this process, may have led to the discrepancy that we see here between the SAD estimated extent and the updated estimated extent of the habitat FOCI 'Subtidal Sands and Gravels'.  As a result of this discrepancy, and in line with recent guidance produced by the JNCC and NE (in prep.), the SAD extent estimate for the habitat FOCI 'Subtidal Sands and Gravels' has been recalculated by summing the extent values of the 'A5.1 Subtidal coarse sediment' and 'A5.2 Subtidal sand' broadscale habitats.  The recalculated estimate for the extent of this FOCI, based on the extent of the SAD broadscale habitats, was 9.65 km2.  There is a large discrepancy between the original (328.44 km2) and recalculated (9.65 km2) extent estimates for this FOCI, which is the result of the different methods of extent estimation. The updated habitat map shows that the majority of the site is comprised of the broadscale habitat 'A5.3 Subtidal mud' and therefore has been classified as containing the habitat FOCI 'Mud Habitats in Deep Water' as specified in Appendix 8 
of the ENG guidance (Natural England and JNCC, 2010).  Figure 6 includes the interpolated sections of Habitat FOCI, although these extents have not been included in the spatial extent calculations. Evidence of sea-pens and burrowing megafauna were identified in the video and still image analysis at a number of sampling stations (Figure 6).  These included general observations of burrows and casts within the muddy substratum, as well as identification of sea-pens (*Vigularia mirabilia*), burrowing anemones (such as Cerianthus lloydii), burrowing crustaceans (e.g. *Nephrops norvegicus*) and burrowing worms (e.g. Sabellida sp.).  Evidence of the habitat FOCI 'Sea-Pen and Burrowing Megafauna Communities' was identified in the biological data from all the video tow sites, and in 112 of the 164 still images collected within the rMCZ site.  This FOCI was evidenced by a single element, or combination of elements from the following list: burrows and/or casts in the sediment; burrowing species of worm, crustacean, or anemone; sea-pen(s).  One or more of these features was identified in each of the images data sources that were classified as containing this habitat FOCI.  The seabed image data suggests that this habitat FOCI is distributed widely throughout the rMCZ area, particularly coinciding with areas of 'Mud Habitats in Deepwater' habitat FOCI, however it was not felt sensible to extrapolate an areal coverage estimate for the 'Sea-Pen and Burrowing Megafauna Communities' habitat FOCI from the point records in the image data. 


| Spatial extent        | Spatial extent        |
|-----------------------|-----------------------|
| according to the      | according to the      |
| Habitat FOCI          | SAD                   |
| within the sub-area   |                       |
| of new survey data    | updated habitat map   |
| Mud Habitats in       |                       |
| Deepwater             | 0 km                  |
| 2                     |                       |
|                       | 0 km                  |
| 2                     |                       |
|                       | 253.34 km             |
| 2                     |                       |
|                       |                       |
| Subtidal Sands and    |                       |
| Gravels*              | 9.65 km               |
| 2                     |                       |
|                       | 9.65 km               |
| 2                     |                       |
|                       | 50.51 km              |
| 2                     |                       |
|                       |                       |
|                       | Sea-pen and Burrowing |
| Megafauna Communities |                       |
| 0 km                  |                       |
| 2                     |                       |
|                       | 0 km                  |
| 2                     |                       |
|                       | Unknown**             |

*The extent of this habitat FOCI has been re-calculated by summing the extent estimates of the 
'A5.1 Subtidal coarse sediment' and 'A5.2 Subtidal sand' BSHs given in the SAD. 

**The presence of this Habitat FOCI is known from the video and still images, but the extent of its coverage has not been estimated. 

## 4.6 Species Foci Identified

There were no species FOCI identified in either the grab or video samples, however, a number of notable species of conservation interest were identified from the image analysis, although these species are not listed as FOCI for the rMCZ network.  The UK Biodiversity Action Plan (BAP) priority species *Pachycerianthus multiplicatus* and Arachnanthus sarsi (http://jncc.defra.gov.uk/page-5167) were recorded on one occasion each (at sampling stations CS01_STN_201 and CS05_STN197 respectively).  *P. multiplicatus* is a large, burrowing, fireworks anemone reported to be generally confined to a few of the Scottish sea lochs and some sheltered inlets off the west of Ireland (JNCC, 2010).  *A. sarsi* is considered to be a very rare anemone, which has only previously been reported in shallower waters (10 - 36 m) around western Scotland and north-west Ireland (JNCC, 2010a).  A further rare species of anemone, *Mescmaea mitchellii,* was identified in the image data from station CS01_STN201.  This species has only been recorded from relatively few locations previously (Wood, 2005).  Additionally, broken shell fragments of the fan mussel Atrina fragilis were also identified in the image data from the same site (CS01_STN201).  This is considered to be one of the most endangered molluscs in UK waters and is the subject of a Species Action Plan (SAP) (JNCC, 2010b). 

|                                      | Previously recorded within    | Identified during evidence    |
|--------------------------------------|-------------------------------|-------------------------------|
| Species FOCI                         | rMCZ                          | gathering survey              |
| Low or Limited Mobility Species FOCI | None recorded                 | None recorded                 |
| Highly Mobile Species FOCI           | None recorded                 | None recorded                 |

## 4.7 Quality Assurance (Qa) And Quality Control (Qc) 4.7.1 Acoustic Data

The acoustic data utilised for production of the updated habitat map were collected and processed in compliance with the International Hydrographic Organisation (IHO) Standards for Hydrographic Surveys - Order 1 (Special Publication 44, Edition 4). The accompanying multibeam backscatter data were reviewed and processed by specialist Cefas staff to ensure these data were suitable for use in the subsequent interpretations and production of the updated habitat map. 

## 4.7.2 Particle Size Analysis (Psa) Of Sediments

PSA was carried out by Kenneth Pye Associated Ltd. and Cefas, following standard laboratory practice and the results checked by specialist Cefas staff following the recommendations of the National Marine Biological Analytical Quality Control (NMBAQC) scheme (Mason, 2011).  Results of the PSA are shown in Appendix 5. 

## 4.7.3 Infaunal Samples From Grabs

Infaunal samples were processed by Thompson Unicomarine Ltd. and MES Ltd. following standard laboratory practices and results checked following the recommendations of the National Marine Biological Analytical Quality Control (NMBAQC) scheme (Worsfold and Hall, 2010). 

## 4.7.4 Video And Still Images And Analysis

Video and photographic stills were processed by Envision Mapping in accordance with the guidance documents developed by Cefas and the JNCC for the acquisition and processing of video and stills data (Coggan and Howell, 2005; JNCC, in prep.; summarised in Annex 5). 

## 4.8 Data Limitations And Adequacy Of The Updated Habitat Map

A source of potential misclassification of habitats arises from the location of groundtruthing samples in relation to habitat types, as well as possible errors on the exact position of grab sample locations, which are plotted as the GPS-derived position of the ship at the time of grab deployment, but in reality some offset may apply. The survey has provided substantial, robust evidence for the presence of the four mapped broad scale habitats (Figure 4).  However, as it is impractical (and undesirable) to sample the entire area of the site with grabs and video, there is a chance that a BSH or FOCI may exist within the site that has not been recorded, especially if it was limited in extent. The precise location of the boundaries between the broadscale habitats depicted on the map should be regarded as indicative, not definitive.  In nature, such boundaries are rarely abrupt.  Instead it is typical for one BSH to grade into another across a transitional boundary.  In contrast, the mapped boundaries are abrupt and have been placed using best professional judgment.  This may have implications when calculating the overall extent of any of the mapped habitats or FOCI. It should be noted that there was a relatively large area of the rMCZ site where no acoustic data were collected (92.83 km2), and therefore broadscale habitats and habitat FOCI were interpolated these areas of the site. Information from the video and still image data from the 2012 research cruises was used to inform the location of the habitat FOCI 'Sea-Pen and Burrowing Megafauna Communities', and these have been included as point records in the FOCI habitat map (Figure 4).  The image data provides strong evidence for the presence of 'Sea- Pen and Burrowing Megafauna Communities' habitat FOCI throughout the rMCZ area, although they cannot support an assessment of the extent of this FOCI habitat. The extent of this type of habitat FOCI cannot be determined from the acoustic data, as it relies on the identification of specific biological communities. 

## 4.8.1 Presence Of Species Foci

No species FOCI were included in the recommendations for proposal of this rMCZ, and no evidence of species FOCI was found in the updated analysis for this report. 

## 4.9 Observations Of Human Impacts On The Seabed

A number of trawl marks have been identified in the acoustic backscatter data and are outlined in the map in Appendix 3.  This provides evidence of demersal fishing activity occurring within this site. 

## 5 Conclusions 5.1 Presence And Extent Of Broadscale Habitats 5.1.1 Presence

 The 2012 dedicated survey has confirmed the presence of the 'A5.1 Subtidal 
coarse sediment', 'A5.2 Subtidal sand' and 'A5.3 Subtidal mud' broadscale habitats that were included in the recommendations made by the SAD for designating this site as an MCZ. 
 There were no broadscale habitats identified in the SAD which were not 
identified in the 2012 dedicated surveys. 
 The 2012 dedicated survey has confirmed the presence of 'A5.4 Subtidal 
mixed sediments' broadscale habitat.  This BSH was not included in the recommendations made by the SAD for designating this site as an MCZ. 

## 5.1.2 Extent

 The spatial extent of the 'A5.1 Subtidal coarse sediment' BSH on the updated 
habitat map is 13.66 km2.  This is an increase of 9.91 km2 when compared with the spatial extent estimated in the SAD habitat map (within the sub-area with data coverage from the 2012 surveys). 
 The spatial extent of the 'A5.2 Subtidal sand' BSH on the updated habitat map 
is 36.85 km2.  This is an increase of 30.95 km2 on the spatial extent estimated in the SAD habitat map (within the sub-area with data coverage from the 2012 surveys). 
 The spatial extent of the 'A5.3 Subtidal mud' BSH on the updated habitat map 
is 253.34 km2, which is a decrease of 42.09 km2 when compared with the estimated extent in the SAD habitat map (within the sub-area with data coverage from the 2012 surveys). 
 The spatial extent of the 'A5.4 Subtidal mixed sediments' BSH on the updated 
habitat map is 1.15 km2. This BSH was not identified in the SAD habitat map. 

## 5.2 Presence And Extent Of Habitat Foci 5.2.1 Presence

 The 2012 dedicated survey has confirmed the presence of the 'Subtidal 
Sands and Gravel' habitat FOCI that was included in the recommendations made by the SAD for designating this site as an MCZ. 
 The 2012 dedicated survey has confirmed the presence of the 'Mud Habitats 
in Deepwater' and 'Sea-pen and Burrowing Megafauna Communities' habitat FOCI at this site.  These habitat FOCI were not included in the recommendations made by the SAD for designating this site as an rMCZ. 

## 5.2.2 Extent And Distribution

 The spatial extent of the 'Subtidal Sands and Gravel' habitat FOCI on the 
updated habitat map is 50.51 km2.  This is 40.86 km2 greater than the spatial extent in the SAD habitat map (within the sub-area with data coverage from the 2012 surveys).  Note that the SAD extent estimate for this FOCI was 
recalculated by summing the extents of the 'A5.1 Subtidal coarse sediment' 
and 'A5.2 Subtidal sand' BSHs, in accordance with recent guidance from the JNCC and NE (in prep.). 
 The spatial extent of the 'Mud Habitats in Deepwater' habitat FOCI on the 
updated habitat map is 253.34 km2.  This habitat FOCI was not identified in the SAD habitat map. 
 There is strong evidence to support the presence of the 'Sea-pen and 
Burrowing Megafauna Communities' habitat FOCI on the updated habitat map, however the extent of this FOCI cannot be estimated accurately from the image data.  This habitat FOCI was not identified in the SAD habitat map. 

## 5.3 Presence And Distribution Of Species Foci 5.3.1 Low Or Limited Mobility Species

 No low or limited mobility species FOCI were recorded at this site by the 2012 
dedicated survey.  These observations are consistent with the evidence presented in the SAD. 

## 5.3.2 Highly Mobile Species Foci

 No highly mobile species FOCI were recorded at this site by the 2012 
dedicated survey.  These observations are consistent with the evidence presented in the SAD. 

## 5.4 Evidence Of Human Activities Impacting The Seabed

There is evidence from the multibeam backscatter data of demersal fishing activity occurring within the rMCZ in the form of trawl marks, visible in the acoustic data (Appendix 3). 

## References

Burrough, P.A. and McDonnell, R.A. (1998). Principles of Geographical Information 
Systems, Oxford University Press, New York, 190 pp. 
Coggan, R. (2012) North West of Jones Bank rMCZ Survey Report, Cefas Report 
Ref: JB(NW)/06/AB/07-12.  Project code: C5785A. 
Coggan, R., Mitchell, A., White, J. and Golding, N. (2007).  Recommended operating 
guidelines (ROG) for underwater video and photographic imaging techniques (www.searchmesh.net/PDF/GMHM3_video_ROG.pdf) [Accessed 24/01/2014] 
Coggan, R. and Howell, K. (2005).  Draft SOP for the collection and analysis of video 
and still images for groundtruthing an acoustic basemap.  Video survey SOP 
version 5, 10 pp. 
Gardline (2012), CEFAS (on behalf of Defra) Lot-6 - Northwest Jones Bank Defra 
MCZ Programme 2012 Acquisition Report March 2012, Gardline Project Ref: 9060. 
Jenness, J.S. (2004).  Calculating landscape surface area from digital elevation 
models.  Wildlife Society Bulletin. 32, 829-839. 
JNCC (in prep.).  Video/Stills Camera Standard Operating Procedure for Survey and 
Analysis: for groundtruthing and classifying an acoustic basemap, and development of new biotopes within the UK Marine Habitat Classification. JNCC Video and Stills Processing SOP v2.  6 pp. 
JNCC (2010) http://jncc.defra.gov.uk/_speciespages/2480.pdf, [Accessed 
24/01/2014]. 
JNCC (2010a) http://jncc.defra.gov.uk/_speciespages/2051.pdf, [Accessed 
24/01/2014]. 
JNCC (2010b) http://jncc.defra.gov.uk/_speciespages/123.pdf, [Accessed 
24/01/2014]. 
JNCC and Natural England (2012).  Marine Conservation Zone Project:  JNCC and 
Natural England's advice to Defra on recommended Marine Conservation Zones.  Peterborough and Sheffield.  1455 pp. 
Lieberknecht, L.M., Hooper, T.E.J., Mullier, T.M., Murphy, A., Neilly, M., Carr, H., 
Haines, R., Lewin, S. and Hughes, E. (2011) Finding Sanctuary final report and recommendations. A report submitted be the Finding Sanctuary stakeholder project to Defra, the Joint Nature Conservation Committee, and Natural England.  Available at www.finding-sanctuary.org / The UK National Archives http://tna.europearchive.org/*/http://www.finding-sanctuary.org/ [Accessed 24/01/2014] 
Mason, C. (2011).  NMBAQC's Best Practice Guidance Particle Size Analysis (PSA) 
for Supporting Biological Analysis. 
McBreen, F. (2010).  UKSeaMap 2010 EUNIS model Version 3.0. UKSeaMap 2010: 
Predictive seabed habitat map (v5). JNCC. 
Natural England and the Joint Nature Conservation Committee (2010).  The Marine 
Conservation Zone Project: Ecological Network Guidance.  Sheffield and Peterborough, UK. 
Natural England and the Joint Nature Conservation Committee (2012).  SNCB MCZ 
Advice Project-Assessing the scientific confidence in the presence and extent 
of features in recommended Marine Conservation Zones (Technical Protocol E) 
Seeley, B., Higgs, S., Lear, D., Evans, J., Neilly, M., Campbell, M., Wilkes, P. Abd 
Adams, L. (2010). Accessing and Developing the Required Biophysical Dataset and Data Layers for Marine Protected Areas Network Planning and Wider Marine Spatial Planning Purposes. Report No 16: Mapping of Protected Habitats (MB102 Task 2C). Defra, London. 
Wood, C. (2005) Seasearch Guide to Sea Anemones and Corals of Britain and 
Ireland.  Marine Conservation Society, Ross-on-Wye. 
Worsfold, T.M., Hall., D.J. and O'Reilly, M. (2010).  Guidelines for processing marine 
macrobenthic invertebrate samples: a processing requirements protocol version 1 (June 2010).  Unicomarine Report NMBAQCMbPRP to the NMBAQC Committee. 33pp.http://www.nmbaqcs.org/media/9732/nmbaqc%20- %20inv%20-%20prp%20-%20v1.0%20june2010.pdf [Accessed 24/01/2014]. 

## Data Sources

All enquiries in relation to this report should be addressed to following e-mail address:  marinescience@defra.gsi.gov.uk 

## Annexes Annex 1.  Broadscale Habitat Features Listed In The Eng.

| Broadscale Habitat Type                               | EUNIS Level 3 Code    |
|-------------------------------------------------------|-----------------------|
| High Energy Intertidal Rock                           | A1.1                  |
| Moderate Energy Intertidal Rock                       | A1.2                  |
| Low Energy Intertidal Rock                            | A1.3                  |
| Intertidal Coarse Sediment                            | A2.1                  |
| Intertidal Sand and Muddy Sand                        | A2.2                  |
| Intertidal Mud                                        | A2.3                  |
| Intertidal Mixed Sediments                            | A2.4                  |
| Coastal Saltmarshes and Saline Reed Beds              | A2.5                  |
| Intertidal Sediments Dominated by Aquatic Angiosperms | A2.6                  |
| Intertidal Biogenic Reefs                             | A2.7                  |
| High Energy Infralittoral Rock*                       | A3.1                  |
| Moderate Energy Infralittoral Rock*                   | A3.2                  |
| Low Energy Infralittoral Rock*                        | A3.3                  |
| High Energy Circalittoral Rock**                      | A4.1                  |
| Moderate Energy Circalittoral Rock**                  | A4.2                  |
| Low Energy Circalittoral Rock**                       | A4.3                  |
| Subtidal Coarse Sediment                              | A5.1                  |
| Subtidal Sand                                         | A5.2                  |
| Subtidal Mud                                          | A5.3                  |
| Subtidal Mixed Sediment                               | A5.4                  |
| Subtidal Macrophyte Dominated Sediment                | A5.5                  |
| Subtidal Biogenic Reef                                | A5.6                  |
| Deep Seabed***                                        | A6                    |

* Infralittoral rock includes habitats of bedrock, boulders and cobble which occur in the shallow subtidal zone and typically support seaweed communities ** Circalittoral rock is characterised by animal dominated communities, rather than seaweed dominated communities *** The deep seabed broadscale habitat encompasses several different habitat sub-types, all of which should be protected within the MPA network.  The broadscale habitat deep seabed habitat is found only in the south-west of the MCZ project area and MCZs identified for this broadscale habitat should seek to protect the variety of sub-types known to occur in the region. 

## Annex 2.  Habitat Foci Listed In The Eng.

Habitat Features of Conservation Importance (FOCI) 
Blue Mussel Beds (including intertidal beds on mixed and sandy sediments)** Coldwater Coral Reefs *** Coral Gardens*** Deepsea Sponge Aggregations*** Estuarine Rocky Habitats File Shell Beds*** Fragile Sponge and Anthozoan Communities on Subtidal Rocky Habitats Intertidal Underboulder Communities Littoral Chalk Communities Maerl Beds Horse Mussel (*Modiolus modiolus*) Beds 
Mud Habitats in Deepwater Sea-pen and Burrowing Megafauna Communities Native Oyster (*Ostrea edulis*) Beds Peat and Clay Exposures Honeycomb Worm (*Sabellaria alveolata*) reefs Ross Worm (*Sabellaria spinulosa*) reefs Seagrass Beds Sheltered Muddy Gravels Subtidal Chalk Subtidal Sands and Gravels Tide-Swept Channels 

* Habitat FOCI have been identified from the 'OSPAR List of Threatened and/or Declining Species and Habitats' and the 'UK List of Priority Species and Habitats (UK BAP)'. ** Only includes 'natural' beds on a variety of sediment types.  Excludes artificially created mussel beds and those which occur on rocks and boulders. *** Coldwater coral reefs, coral gardens, deep sea sponge aggregations and file shell beds currently do not have distributional data which demonstrate their presence within the MCZ project area. 

## Annex 3.  Low Or Limited Mobility Species Foci Listed In The Eng.

| Group                         |
|-------------------------------|
| Brown Algae                   |
| Padina pavonica               |
| Peacock's Tail                |
| Red Algae                     |
| Cruoria cruoriaeformis        |
| Grateloupia montagnei         |
| Lithothamnion corallioides    |
| Phymatolithon calcareum       |
| Burgundy Maerl Paint Weed     |
| Grateloup's little-Lobed Weed |
| Coral Maerl                   |
| Common Maerl                  |
| Annelida                      |
| Alkmaria romijni**            |
| Armandia cirrhosa**           |
| Tentacled Lagoon Worm**       |
| Lagoon Sand Worm**            |
| Teleostei                     |
| Gobius cobitis                |
| Gobius couchi                 |
| Hippocampus guttulatus        |
| Hippocampus hippocampus       |
| Giant Goby                    |
| Couch's Goby                  |
| Long Snouted Seahorse         |
| Short Snouted Seahorse        |
| Bryozoa                       |
| Victorella pavida             |
| Trembling Sea Mat             |
| Cnidaria                      |
| Amphianthus dohrnii           |
| Eunicella verrucosa           |
| Haliclystus auricula          |
| Leptosammia pruvoti           |
| Lucernariopsis campanulata    |
| Lucernariopsis cruxmelitensis |
| Nematostella vectensis        |
| Sea Fan Anemone               |
| Pink Sea Fan                  |
| Stalked jellyfish             |
| Sunset Cup Coral              |
| Stalked Jellyfish             |
| Stalked Jellyfish             |
| Starlet Sea Anemone           |
| Crustacea                     |
| Gammarus insensibilis**       |
| Gitanopsis bispinosa          |
| Pollicipes pollicipes         |
| Palinurus elephas             |
| Lagoon Sand Shrimp**          |
| Amphipod                      |
| Gooseneck Barnacle            |
| Spiny Lobster                 |
| Mollusca                      |
| Arctica islandica             |
| Atrina pectinata              |
| Caecum armoricum**            |
| Ostrea edulis                 |
| Paludinella littorina         |
| Tenellia adspersa**           |
| Ocean Quahog                  |
| Fan Mussel                    |
| Defolin's Lagoon Snail**      |
| Native Oyster                 |
| Sea Snail                     |
| Lagoon Sea Slug**             |

* Species FOCI have been identified from the 'OSPAR List of Threatened and/or Declining Species and Habitats', the 'UK List of Priority Species and Habitats (UK BAP)' and Schedule 5 of the Wildlife and Countryside Act. 

** Those lagoonal species FOCI may be afforded sufficient protection through coastal lagoons designated as SACs under the EC Habitats Directive.  However, this needs to be assessed by individual regional projects. 

## Annex 4.  Highly Mobile Species Foci Listed In The Eng.

| Group             |
|-------------------|
| Teleostei         |
| Osmerus eperlanus |
| Anguilla anguilla |
| Smelt             |
| European Eel      |
| Elasmobranchii    |
| Raja undulata     |
| Undulate Ray      |

* Species FOCI have been identified from the 'OSPAR List of Threatened and/or Declining Species and Habitats', the 'UK List of Priority Species and Habitats (UK BAP)' and Schedule 5 
of the Wildlife and Countryside Act. 

## Annex 5.  Video And Stills Processing Protocol.

The purpose of the analysis of the video and still images is to identify what habitats exist in a video record, provide semi-quantitative data on their physical and biological characteristics and to note where one habitat changes to another.  A minimum of 10% of the videos should be re-analysed for QA purposes. 

## Video Analysis

 The video record is initially viewed rapidly (at approximately 4x normal speed) 
in order to segment it into sections representing different habitats.  The start and end points of each segment are logged, and each segment subsequently subject to more detailed analysis.  Brief changes in habitat type lasting less than one minute of the video record are considered as incidental patches and are not logged. 
 For each segment, note the start and end time and position from the 
information on the video overlay.  View the segment at normal or slower than normal speed, noting the physical and biological characteristics, such as substrate type, seabed character, species and life forms present.  For each taxon record an actual abundance (where feasible) or a semi quantitative abundance (e.g. SACFOR scale). 
 Record the analyses on the video pro-forma provided (paper and/or 
electronic), which is a modified version of the Sublittoral Habitat Recording Form used in the MNCR surveys. 
 When each segment has been analysed, review the information recorded and 
assign the segment to one of the broadscale habitat (BSH) types or Habitat FOCI listed in the Ecological Network Guidance (as reproduced in Annexes 1 and 2 above).  Note also any species FOCI observed (as per Annex 3 above). 

## Stills Analysis

 Still images should be analysed separately, to supplement and validate the 
video analysis, and provide more detailed (i.e. higher resolution) information 
than can be extracted from a moving video image.  
 For each segment of video, select three still images that are representative of 
the BSH or FOCI to which the video segment has been assigned.  For each image, note the time and position it was taken, using information from the associated video overlay.  
 View the image at normal or greater than normal magnification, noting the 
physical and biological characteristics, such as substrate type, seabed character, species and life forms present.  For each taxon record an actual abundance (where feasible) or a semi quantitative abundance (e.g. SACFOR scale).  
 Record the analysis on the stills pro-forma provided (paper and/or electronic), 
which is a modified version of the Sublittoral Habitat Recording Form used in the MNCR surveys.  Assign each still image to the same BSH or Habitat FOCI as its 'parent' segment in the video. 

## Taxon Identification

In all analyses, the identification of taxa should be limited to a level that can be confidently achieved from the available image.  Hence, taxon identity could range from the 'life form' level (e.g. sponge, hydroid, anemone) to the species level (e.g. Asterias rubens, *Alcyonium digitatum*).  Avoid the temptation to guess the species identity if it can not be determined positively from the image.  For example, Pomatoceros sp. would be acceptable, but *Pomatoceros triqueter* would not, as the specific identification normally requires the specimen to be inspected under a microscope. 

## Appendices Appendix 1. Survey Metadata (Gardline Cruise (Job #9060) And Cend10/12))

| Date       |    Cruise  | Stn No.     | Stn Code    | Gear          | Latitude    | Longitude    |
|------------|------------|-------------|-------------|---------------|-------------|--------------|
| 29/03/2012 | 9060       | JB(NW)ENV01 | ENV01       | Day Grab      | 49.83201    | -8.15458     |
| 20/03/2012 | 9060       | JB(NW)ENV02 | ENV02       | Day Grab      | 49.83989    | -8.30175     |
| 19/03/2012 | 9060       | JB(NW)ENV03 | ENV03       | Day Grab      | 49.84083    | -8.10038     |
| 30/03/2012 | 9060       | JB(NW)ENV06 | ENV06       | Day Grab      | 49.86648    | -8.13914     |
| 29/03/2012 | 9060       | JB(NW)ENV08 | ENV08       | Day Grab      | 49.87529    | -8.0849      |
| 20/03/2012 | 9060       | JB(NW)ENV09 | ENV09       | Day Grab      | 49.88326    | -8.23219     |
| 30/03/2012 | 9060       | JB(NW)ENV10 | ENV10       | Day Grab      | 49.89212    | -8.17794     |
| 20/03/2012 | 9060       | JB(NW)ENV11 | ENV11       | Day Grab      | 49.89996    | -8.3253      |
| 19/03/2012 | 9060       | JB(NW)ENV12 | ENV12       | Day Grab      | 49.90886    | -8.27106     |
| 30/03/2012 | 9060       | JB(NW)ENV13 | ENV13       | Day Grab      | 49.90975    | -8.0694      |
| 30/03/2012 | 9060       | JB(NW)ENV14 | ENV14       | Day Grab      | 49.91774    | -8.21679     |
| 29/03/2012 | 9060       | JB(NW)ENV15 | ENV15       | Day Grab      | 49.92658    | -8.16251     |
| 20/03/2012 | 9060       | JB(NW)ENV16 | ENV16       | Day Grab      | 49.93445    | -8.30997     |
| 19/03/2012 | 9060       | JB(NW)ENV17 | ENV17       | Day Grab      | 49.93541    | -8.10821     |
| 29/03/2012 | 9060       | JB(NW)ENV19 | ENV19       | Day Grab      | 49.95221    | -8.20138     |
| 29/03/2012 | 9060       | JB(NW)ENV20 | ENV20       | Day Grab      | 49.96105    | -8.14706     |
| 29/03/2012 | 9060       | JB(NW)ENV21 | ENV21       | Day Grab      | 49.96893    | -8.29462     |
| 29/03/2012 | 9060       | JB(NW)ENV22 | ENV22       | Day Grab      | 49.96987    | -8.09271     |
| 29/03/2012 | 9060       | JB(NW)ENV23 | ENV23       | Day Grab      | 49.97782    | -8.2403      |
| 19/03/2012 | 9060       | JB(NW)ENV24 | ENV24       | Day Grab      | 49.98668    | -8.18595     |
| 29/03/2012 | 9060       | JB(NW)ENV25 | ENV25       | Day Grab      | 49.99552    | -8.13159     |
| 29/03/2012 | 9060       | JB(NW)ENV26 | ENV26       | Day Grab      | 50.00341    | -8.27926     |
| 29/03/2012 | 9060       | JB(NW)ENV27 | ENV27       | Day Grab      | 50.00433    | -8.0772      |
| 30/03/2012 | 9060       | JB(NW)ENV29 | ENV29       | Day Grab      | 49.82685    | -8.10037     |
| 19/03/2012 | 9060       | JB(NW)ENV30 | ENV30       | Day Grab      | 49.82699    | -8.06263     |
| 30/03/2012 | 9060       | JB(NW)ENV32 | ENV32       | Day Grab      | 49.83015    | -8.08005     |
| 29/03/2012 | 9060       | JB(NW)ENV33 | ENV33       | Day Grab      | 49.83166    | -8.10763     |
| 29/03/2012 | 9060       | JB(NW)ENV36 | ENV36       | HamCam        | 49.83345    | -8.05972     |
| 30/03/2012 | 9060       | JB(NW)ENV37 | ENV37       | Day Grab      | 49.83662    | -8.07715     |
| 30/03/2012 | 9060       | JB(NW)ENV40 | ENV40       | Day Grab      | 49.84472    | -8.06408     |
| 49.90575   |   -8.13096 | 29/03/2012  | 9060        | JB(NW)ENV46   | ENV46       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 49.94559   |   -8.33181 | 28/03/2012  | 9060        | JB(NW)ENV48   | ENV48       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 49.92225   |   -8.09086 | 29/03/2012  | 9060        | JB(NW)ENV50   | ENV50       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 49.94252   |   -8.06141 | 22/03/2012  | 9060        | JB(NW)ENV52   | ENV52       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 49.88855   |   -8.10708 | 29/03/2012  | 9060        | JB(NW)ENV54   | ENV54       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 49.89481   |   -8.14133 | 29/03/2012  | 9060        | JB(NW)ENV55   | ENV55       | Mini Hamon   |
| Grab       |            |             |             |               |             |              |
| 31/03/2012 | 9060       | JB(NW)ENV2  | ENV2        | Camera Sledge | 49.84119    | -8.30144     |
| 31/03/2012 | 9060       | JB(NW)ENV2  | ENV2        | Camera Sledge | 49.84008    | -8.30176     |
| 31/03/2012 | 9060       | JB(NW)ENV2  | ENV2        | Camera Sledge | 49.83893    | -8.30186     |
| Date       |   Cruise  | Stn No.      | Stn Code    | Gear          |   Latitude  |   Longitude  |
|------------|-----------|--------------|-------------|---------------|-------------|--------------|
| 31/03/2012 |     9060  | JB(NW)ENV3   | ENV3        | Camera Sledge |     49.842  |     -8.09972 |
| 31/03/2012 |     9060  | JB(NW)ENV3   | ENV3        | Camera Sledge |     49.8404 |     -8.10047 |
| 31/03/2012 |     9060  | JB(NW)ENV3   | ENV3        | Camera Sledge |     49.8395 |     -8.10082 |
| 31/03/2012 |     9060  | JB(NW)ENV8   | ENV8        | Camera Sledge |     49.8767 |     -8.08438 |
| 31/03/2012 |     9060  | JB(NW)ENV8   | ENV8        | Camera Sledge |     49.8753 |     -8.08486 |
| 31/03/2012 |     9060  | JB(NW)ENV8   | ENV8        | Camera Sledge |     49.8745 |     -8.08562 |
| 31/03/2012 |     9060  | JB(NW)ENV9   | ENV9        | Camera Sledge |     49.8849 |     -8.23204 |
| 31/03/2012 |     9060  | JB(NW)ENV9   | ENV9        | Camera Sledge |     49.8833 |     -8.23215 |
| 31/03/2012 |     9060  | JB(NW)ENV9   | ENV9        | Camera Sledge |     49.8822 |     -8.23223 |
| 30/03/2012 |     9060  | JB(NW)ENV11  | ENV11       | Camera Sledge |     49.9012 |     -8.32485 |
| 30/03/2012 |     9060  | JB(NW)ENV11  | ENV11       | Camera Sledge |     49.9003 |     -8.32516 |
| 30/03/2012 |     9060  | JB(NW)ENV11  | ENV11       | Camera Sledge |     49.8989 |     -8.3262  |
| 30/03/2012 |     9060  | JB(NW)ENV17  | ENV17       | Camera Sledge |     49.9366 |     -8.10651 |
| 30/03/2012 |     9060  | JB(NW)ENV17  | ENV17       | Camera Sledge |     49.9358 |     -8.1075  |
| 30/03/2012 |     9060  | JB(NW)ENV17  | ENV17       | Camera Sledge |     49.935  |     -8.10879 |
| 30/03/2012 |     9060  | JB(NW)ENV17a | ENV17a      | Camera Sledge |     49.9356 |     -8.10782 |
| 30/03/2012 |     9060  | JB(NW)ENV17a | ENV17a      | Camera Sledge |     49.9352 |     -8.10857 |
| 30/03/2012 |     9060  | JB(NW)ENV17a | ENV17a      | Camera Sledge |     49.9347 |     -8.10927 |
| 30/03/2012 |     9060  | JB(NW)ENV19  | ENV19       | Camera Sledge |     49.9533 |     -8.20028 |
| 30/03/2012 |     9060  | JB(NW)ENV19  | ENV19       | Camera Sledge |     49.952  |     -8.20133 |
| 30/03/2012 |     9060  | JB(NW)ENV19  | ENV19       | Camera Sledge |     49.9515 |     -8.20259 |
| 30/03/2012 |     9060  | JB(NW)ENV24  | ENV24       | Camera Sledge |     49.9875 |     -8.1845  |
| 30/03/2012 |     9060  | JB(NW)ENV24  | ENV24       | Camera Sledge |     49.9872 |     -8.18529 |
| 30/03/2012 |     9060  | JB(NW)ENV24  | ENV24       | Camera Sledge |     49.9863 |     -8.18643 |
| 31/03/2012 |     9060  | JB(NW)ENV41  | ENV41       | Camera Sledge |     49.8954 |     -8.12585 |
| 31/03/2012 |     9060  | JB(NW)ENV41  | ENV41       | Camera Sledge |     49.8943 |     -8.12668 |
| 31/03/2012 |     9060  | JB(NW)ENV41  | ENV41       | Camera Sledge |     49.8936 |     -8.1274  |
| 30/03/2012 |     9060  | JB(NW)ENV51  | ENV51       | Camera Sledge |     49.9315 |     -8.06425 |
| 30/03/2012 |     9060  | JB(NW)ENV51  | ENV51       | Camera Sledge |     49.9299 |     -8.06667 |
| 30/03/2012 |     9060  | JB(NW)ENV51  | ENV51       | Camera Sledge |     49.9289 |     -8.06919 |
| 30/03/2012 |     9060  | JB(NW)ENV51a | ENV51a      | Camera Sledge |     49.931  |     -8.0649  |
| 30/03/2012 |     9060  | JB(NW)ENV51a | ENV51a      | Camera Sledge |     49.93   |     -8.0667  |
| 30/03/2012 |     9060  | JB(NW)ENV51a | ENV51a      | Camera Sledge |     49.9285 |     -8.06887 |
| 31/03/2012 |     9060  | JB(NW)ENV55  | ENV55       | Camera Sledge |     49.8957 |     -8.14001 |
| 31/03/2012 |     9060  | JB(NW)ENV55  | ENV55       | Camera Sledge |     49.8947 |     -8.14158 |
| 31/03/2012 |     9060  | JB(NW)ENV55  | ENV55       | Camera Sledge |     49.8941 |     -8.14236 |
| 31/03/2012 |     9060  | JB(NW)ENV02  | ENV2        | Camera Sledge |     49.8399 |     -8.30175 |
| 31/03/2012 |     9060  | JB(NW)ENV03  | ENV3        | Camera Sledge |     49.8408 |     -8.10038 |
| 31/03/2012 |     9060  | JB(NW)ENV08  | ENV8        | Camera Sledge |     49.8753 |     -8.0849  |
| 31/03/2012 |     9060  | JB(NW)ENV09  | ENV9        | Camera Sledge |     49.8833 |     -8.23219 |
| 30/03/2012 |     9060  | JB(NW)ENV11  | ENV11       | Camera Sledge |     49.9    |     -8.3253  |
| 30/03/2012 |     9060  | JB(NW)ENV17  | ENV17       | Camera Sledge |     49.9354 |     -8.10821 |
| 30/03/2012 |     9060  | JB(NW)ENV17  | ENV17a      | Camera Sledge |     49.9354 |     -8.10821 |
| 30/03/2012 |     9060  | JB(NW)ENV19  | ENV19       | Camera Sledge |     49.9522 |     -8.20138 |
| 30/03/2012 |     9060  | JB(NW)ENV24  | ENV24       | Camera Sledge |     49.9867 |     -8.18595 |
| 31/03/2012 |     9060  | JB(NW)ENV41  | ENV41       | Camera Sledge |     49.8957 |     -8.12532 |
| 30/03/2012 |     9060  | JB(NW)ENV51  | ENV51       | Camera Sledge |     49.9482 |     -8.06433 |
| 30/03/2012 |     9060  | JB(NW)ENV51  | ENV51a      | Camera Sledge |     49.9313 |     -8.0645  |
| 31/03/2012 |     9060  | JB(NW)ENV55  | ENV55       | Camera Sledge |     49.8948 |     -8.14133 | | Date       | Cruise    |   Stn No.  | Stn Code    | Gear          |   Latitude  |   Longitude  |
|------------|-----------|------------|-------------|---------------|-------------|--------------|
| 11/07/2012 | CEND10_12 |       185  | NWJB01      | HamCam        |     50.0029 |     -8.28246 |
| 11/07/2012 | CEND10_12 |       192  | NWJB02      | HamCam        |     49.8402 |     -8.30154 |
| 11/07/2012 | CEND10_12 |       190  | NWJB03      | HamCam        |     49.8749 |     -8.2834  |
| 11/07/2012 | CEND10_12 |       193  | NWJB04      | HamCam        |     49.848  |     -8.2534  |
| 11/07/2012 | CEND10_12 |       182  | NWJB05      | HamCam        |     49.9958 |     -8.33203 |
| 11/07/2012 | CEND10_12 |       187  | NWJB06      | HamCam        |     49.9436 |     -8.25406 |
| 12/07/2012 | CEND10_12 |       195  | NWJB07      | HamCam        |     49.8577 |     -8.19338 |
| 12/07/2012 | CEND10_12 |       205  | NWJB08      | HamCam        |     50.0045 |     -8.07729 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9965 |     -8.33064 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9964 |     -8.33086 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9963 |     -8.33098 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9962 |     -8.33116 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9961 |     -8.3313  |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9961 |     -8.33151 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.996  |     -8.33164 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9959 |     -8.33175 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9958 |     -8.33189 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9956 |     -8.33207 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9956 |     -8.33227 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9956 |     -8.33245 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9955 |     -8.33247 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9955 |     -8.33254 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9954 |     -8.33261 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9953 |     -8.33273 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9447 |     -8.2516  |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9447 |     -8.25175 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9446 |     -8.25195 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9444 |     -8.2523  |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9444 |     -8.25246 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9443 |     -8.25262 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9442 |     -8.25281 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9441 |     -8.25298 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.944  |     -8.25311 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.944  |     -8.25315 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.944  |     -8.25334 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9439 |     -8.25351 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9438 |     -8.25369 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9437 |     -8.25383 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9436 |     -8.254   |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9436 |     -8.25419 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9435 |     -8.25439 |
| 12/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9434 |     -8.25455 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9237 |     -8.10547 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9238 |     -8.10542 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.924  |     -8.10532 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9241 |     -8.10522 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9242 |     -8.10517 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9242 |     -8.10515 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9244 |     -8.10507 | | Date       | Cruise    |   Stn No.  | Stn Code    | Gear          |   Latitude  |   Longitude  |
|------------|-----------|------------|-------------|---------------|-------------|--------------|
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9245 |     -8.10499 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9246 |     -8.10493 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9247 |     -8.10487 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9249 |     -8.10476 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.925  |     -8.10466 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9251 |     -8.1046  |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9253 |     -8.10453 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9254 |     -8.10443 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9255 |     -8.1044  |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9255 |     -8.10437 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9256 |     -8.10434 |
| 12/07/2012 | CEND10_12 |       201  | NWJB_CS01   | Camera Sledge |     49.9257 |     -8.10426 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9808 |     -8.07068 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9808 |     -8.07066 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9807 |     -8.07055 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9806 |     -8.07044 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9805 |     -8.0703  |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9804 |     -8.0702  |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9802 |     -8.07008 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9802 |     -8.07003 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9801 |     -8.06993 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.98   |     -8.06984 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9799 |     -8.06973 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9798 |     -8.06962 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9797 |     -8.0695  |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9796 |     -8.06939 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9795 |     -8.06937 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9794 |     -8.06923 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9793 |     -8.06915 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9792 |     -8.06901 |
| 12/07/2012 | CEND10_12 |       203  | NWJB_CS02   | Camera Sledge |     49.9791 |     -8.06889 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8312 |     -8.15629 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8312 |     -8.15625 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8313 |     -8.15612 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8314 |     -8.15594 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8315 |     -8.15579 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8316 |     -8.15562 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8317 |     -8.15543 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8317 |     -8.15539 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8317 |     -8.15529 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8318 |     -8.15522 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8319 |     -8.15507 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8319 |     -8.15496 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.832  |     -8.15477 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.832  |     -8.15474 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8321 |     -8.1546  |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8321 |     -8.15449 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8323 |     -8.15425 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8323 |     -8.15408 |
| Date       | Cruise    |   Stn No.  | Stn Code    | Gear          |   Latitude  |   Longitude  |
|------------|-----------|------------|-------------|---------------|-------------|--------------|
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8324 |     -8.15391 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8325 |     -8.15374 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8325 |     -8.15366 |
| 12/07/2012 | CEND10_12 |       197  | NWJB_CS05   | Camera Sledge |     49.8326 |     -8.15357 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8254 |     -8.06718 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8254 |     -8.06697 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8255 |     -8.0668  |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8256 |     -8.0666  |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8256 |     -8.06656 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8257 |     -8.06642 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8257 |     -8.06624 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8258 |     -8.06611 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8258 |     -8.06597 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8259 |     -8.06587 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8259 |     -8.06568 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.826  |     -8.0655  |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8261 |     -8.0654  |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8261 |     -8.06538 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8261 |     -8.06526 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8261 |     -8.06515 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8262 |     -8.06501 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8262 |     -8.06495 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8263 |     -8.06477 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8264 |     -8.06461 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8264 |     -8.06456 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8264 |     -8.06451 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8264 |     -8.06447 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8265 |     -8.06439 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8265 |     -8.06421 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8266 |     -8.06411 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8266 |     -8.06403 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8267 |     -8.06385 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8267 |     -8.06376 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8267 |     -8.06366 |
| 12/07/2012 | CEND10_12 |       199  | NWJB_CS06   | Camera Sledge |     49.8268 |     -8.06347 |
| 11/07/2012 | CEND10_12 |       183  | NWJB05      | Camera Sledge |     49.9965 |     -8.33064 |
| 11/07/2012 | CEND10_12 |       188  | NWJB06      | Camera Sledge |     49.9447 |     -8.2516  |
| 12/07/2012 | CEND10_12 |       201  | CS01        | Camera Sledge |     49.9237 |     -8.10547 |
| 13/07/2012 | CEND10_12 |       201  | CS01        | Camera Sledge |     49.9242 |     -8.10507 |
| 14/07/2012 | CEND10_12 |       201  | CS01        | Camera Sledge |     49.9247 |     -8.10486 |
| 15/07/2012 | CEND10_12 |       201  | CS01        | Camera Sledge |     49.9251 |     -8.1046  |
| 16/07/2012 | CEND10_12 |       203  | CS02        | Camera Sledge |     49.9808 |     -8.07068 |
| 17/07/2012 | CEND10_12 |       197  | CS05        | Camera Sledge |     49.8312 |     -8.15629 |
| 18/07/2012 | CEND10_12 |       199  | CS06        | Camera Sledge |     49.8254 |     -8.06718 |
|            |           |            |             |               |             |              |

## Appendix 2. Outputs From Acoustic Surveys Appendix 3. Evidence Of Human Activities Within The Rmcz Appendix 4. Species List

Species list for grab samples from both cruises (Species FOCI indicated by grey shading if present).  Percentage occurrence was calculated as the 'Number of samples where the species occurs/total number of samples' x 100. 

Taxa 
% Occurrence 
FORAMINIFERA 
  
Astrorhiza 
2 
CHLOROPHYTA 
  
Enteromorpha 
2 
HYDROIDS, CORALS, JELLYFISH, ANEMONES 
  
FILIFERA 
14 
Cerianthus lloydii 
11 
Edwardsia claparedii 
9 
ACTINIARIA 
4 
Edwardsiidae 
2 
FLATWORMS 
  
Monticellina 
7 
TURBELLARIA 
7 
RIBBONWORMS 
  
NEMERTEA 
50 
ROUNDWORMS 
  
NEMATODA 
14 
ARROW WORMS 
  
Sagittidae 
7 
PEANUT WORMS 
  
Aspidosiphon muelleri 
2 
Golfingia elongata 
2 
Phascolion strombus 
2 
SEGMENTED WORMS 
  
Dasybranchus 
82 
Terebellides stroemi 
59 
Nephtys hystricis 
55 
Spiophanes kroyeri 
55 
Galathowenia oculata 
50 
Glycera rouxii 
50 
Prionospio dubia 
50 
Abyssoninoe hibernica 
48 
Praxillella affinis (Type A) 
48 
Notomastus 
32 
Magelona minuta 
30 
Amphicteis gunneri 
27 
Lumbrineris gracilis 
27 
Nephtys incisa 
27 
Diplocirrus glaucus 
25 
Ampharete lindstroemi 
23 
Peresiella clymenoides 
23 
Taxa 
% Occurrence 
Glycinde nordmanni 
21 
Ampharete falcata 
18 
Scoloplos armiger 
18 
Aricidea catherinae 
16 
Aricidea laubieri 
16 
Eclysippe vanelli 
14 
Maldanidae 
14 
Aponuphis bilineata 
11 
Jasmineira elegans 
11 
Aglaophamus rubella 
9 
Goniada maculata 
9 
Nephtys hombergii 
9 
Nephtys kersivalensis 
9 
Polycirrus 
9 
Prionospio fallax 
9 
Scalibregma inflatum 
9 
Sthenelais limicola 
9 
Eunereis longissima 
7 
Glycera fallax 
7 
Glycera lapidum (agg) 
7 
Malmgrenia arenicolae 
7 
Mediomastus fragilis 
7 
Minuspio multibranchiata 
7 
Cirrophorus branchiatus 
5 
Clymenura 
5 
Ditrupa arietina 
5 
Euchone 
5 
Eulalia mustela 
5 
Glycera alba 
5 
Nephtys (juv) 
5 
Ophelina acuminata 
5 
Poecilochaetus serpens 
5 
Sphaerosyllis bulbosa 
5 
Streblosoma bairdi 
5 
Trichobranchus roseus 
5 
Ancistrosyllis groenlandica 
4 
Amaeana trilobata 
2 
Amphictene auricoma 
2 
Aonides paucibranchiata 
2 
Autolytus 
2 
Glycinde nordmanni (epitoke) 
2 
Goniada maculata (epitoke) 
2 
Goniadella gracilis 
2 
Goniadella gracilis (epitoke) 
2 
Grania 
2 
Harmothoe fernandi (?) 
2 
Taxa 
% Occurrence 
Jasmineira caudata 
2 
Laonice bahusiensis 
2 
Marphysa kinbergi (?) 
2 
Mesochaetopterus 
2 
Nicolea venustula 
2 
Ophelia borealis 
2 
Ophelina cylindricaudata 
2 
Owenia fusiformis 
2 
Paradoneis eliasoni 
2 
Pisione remota 
2 
Podarkeopsis capensis 
2 
Polygordius 
2 
Scolelepis korsuni 
2 
Scoletoma magnidentata 
2 
Sige fusigera 
2 
Spiophanes bombyx 
2 
SEA SPIDERS 
  
Paranymphon spinosum 
5 
CRUSTACEANS 
  
Ampelisca spinipes 
55 
Goneplax rhomboides 
30 
COPEPODA 
23 
Unciola planipes 
18 
Ampelisca spinifer 
14 
Ampelisca tenuicornis 
9 
Paguridae (juv) 
9 
Alpheus glaber 
7 
Solenocera membranacea 
7 
Cheirocratus 
5 
Ebalia (Juv) 
5 
Siphonoecetes striatus 
5 
Acidostoma obesum (sensu Stoddart & Lowry) 
2 
Ampelisca 
2 
Atelecyclus rotundatus (juv) 
2 
Callianassa subterranea (juv) 
2 
DECAPODA 
2 
Eriopisa elongata 
2 
Eudorella truncatula 
2 
Eusirus longipes 
2 
Hippomedon denticulatus 
2 
Makrokylindrus (juv) 
2 
Natatolana borealis 
2 
Perioculodes longimanus 
2 
Processa nouveli 
2 
Tmetonyx 
2 
Taxa 
% Occurrence 
MOLLUSCS 
  
Thyasira polygona 
57 
Abra nitida 
52 
Nucula sulcata 
43 
Corbula gibba 
30 
Falcidens crossotus 
27 
Myrtea spinifera 
14 
Phaxas pellucidus 
11 
Mangelia brachystoma 
9 
Alvania abyssicola 
7 
Parvicardium minimum 
7 
Euspira pulchella 
5 
Axinulus croulinensis 
2 
Cylichna cylindracea 
2 
Epitonium trevelyanum 
2 
Euspira fusca 
2 
Kurtiella tumidula 
2 
Lucinoma borealis 
2 
Lucinoma borealis (juv) 
2 
Mytilus edulis (juv) 
2 
PELECYPODA 
2 
Spisula (juv) 
2 
Turritella communis 
2 
BRYOZOANS 
  
Triticella flava 
5 
Escharella immersa 
2 
Porella concinna 
2 
ENTOPROCTA 
  
Loxosomella varians 
50 
HORSESHOE WORMS 
  
Phoronis 
18 
SEA STARS, URCHINS, SEA CUCUMBERS 
  
Amphiuridae (juv) 
18 
Echinocyamus pusillus 
14 
Ophiuridae (juv) 
9 
SPATANGOIDA (juv) 
9 
Amphiura filiformis 
5 
Amphipholis squamata 
2 
Astropecten irregularis 
2 
Echinocardium flavescens 
2 
Ophiura albida 
2 
SPATANGOIDA 
2 
FISH 
  
OSTEICHTHYES (eggs) 
2 
 

Species list for video samples (Species FOCI indicated by grey shading if present). Percentage occurrence was calculated as the 'Number of video samples where the species occurs/total number of video samples' x 100. 

| Taxa                                  |
|---------------------------------------|
| SPONGES                               |
|                                       |
|                                       |
| Porifera                              |
|                                       |
| 4                                     |
|                                       |
| HYDROIDS, CORALS, JELLYFISH, ANEMONES |
|                                       |
|                                       |
| Anthozoa                              |
|                                       |
| 58                                    |
|                                       |
| Cerianthus lloydii                    |
|                                       |
| 54                                    |
|                                       |
| Virgularia mirabilis                  |
|                                       |
| 25                                    |
|                                       |
| Edwardsia                             |
|                                       |
| 13                                    |
|                                       |
| Adamsia                               |
|                                       |
| 8                                     |
|                                       |
| Pachycerianthus                       |
|                                       |
| 8                                     |
|                                       |
| Arachnanthus sarsi                    |
|                                       |
| 4                                     |
|                                       |
| Ctenophora                            |
|                                       |
| 4                                     |
|                                       |
| Mesacmaea mitchellii                  |
|                                       |
| 4                                     |
|                                       |
| Peachia                               |
|                                       |
| 4                                     |
|                                       |
| SEGMENTED WORMS                       |
|                                       |
|                                       |
| Sabella pavonina                      |
|                                       |
| 38                                    |
|                                       |
| Ditrupa arietina                      |
|                                       |
| 33                                    |
|                                       |
| Myxicola infundibulum                 |
|                                       |
| 4                                     |
|                                       |
| Nephtys                               |
|                                       |
| 4                                     |
|                                       |
| CRUSTACEANS                           |
|                                       |
|                                       |
| Caridea                               |
|                                       |
| 67                                    |
|                                       |
| Nephrops norvegicus                   |
|                                       |
| 46                                    |
|                                       |
| Pagurus prideaux                      |
|                                       |
| 33                                    |
|                                       |
| Munida rugosa                         |
|                                       |
| 17                                    |
|                                       |
| Pagurus                               |
|                                       |
| 13                                    |
|                                       |
| Liocarcinus depurator                 |
|                                       |
| 8                                     |
|                                       |
| Brachyuran                            |
|                                       |
| 4                                     |
|                                       |
| Cumacean/Euphasid                     |
|                                       |
| 4                                     |
|                                       |
| Macropodia                            |
|                                       |
| 4                                     |
|                                       |
| MOLLUSCS                              |
|                                       |
|                                       |
| Aequipecten opercularis               |
|                                       |
| 25                                    |
|                                       |
| Turritella communis                   |
|                                       |
| 21                                    |
|                                       |
| Turritella                            |
|                                       |
| 8                                     |
|                                       |
| Atrina fragilis                       |
|                                       |
| 4                                     |
|                                       |
| Octopoda                              |
|                                       |
| 4                                     |
|                                       |
| SEA STARS, URCHINS, SEA CUCUMBERS     |
|                                       |
|                                       |
| Asterias rubens                       |
|                                       |
| 17                                    |
|                                       |
| Ophiura                               |
|                                       |
| 13                                    |
|                                       |
| Ophiura albida                        |
|                                       |
| 13                                    |
|                                       |
| Astropecten irregularis               |
|                                       |
| 8                                     |
|                                       |
| Ophiura ophiura                       |
|                                       |
| 8                                     |
|                                       |
| Asteroidea                            |
|                                       |
| 4                                     |
|                                       |
| Luidia ciliaris                       |
|                                       |
| 4                                     |
|                                       |
| Taxa                       |
|----------------------------|
| Unidentified starfish      |
|                            |
| 4                          |
|                            |
| FISH                       |
|                            |
|                            |
| Glyptocephalus cynoglossus |
|                            |
| 38                         |
|                            |
| Gadidae                    |
|                            |
| 25                         |
|                            |
| Unidentified fish          |
|                            |
| 13                         |
|                            |
| Chondrichthyes             |
|                            |
| 8                          |
|                            |
| Pisces                     |
|                            |
| 8                          |
|                            |
| Ammodytes                  |
|                            |
| 4                          |
|                            |
| Gobiidae                   |
|                            |
| 4                          |
|                            |
| Phycis blennoides          |
|                            |
| 4                          |
|                            |
| Soleidae                   |
|                            |
| 4                          |
|                            |
|                            |

## Appendix 5. Analyses Of Sediment Samples: Classification And Composition

Stn No. 
Stn Code 
Latitude 
Longitude 
Sediment Description 
EUNIS Level 3/BSH 
Gravel (%) 
Sand (%) 
Silt/clay (%) 
JB(NW)ENV01 
ENV01 
49.832011 
-8.154578 
mud and sandy mud 
A5.3 Subtidal Mud 
0.50 
73.06 
26.44 
JB(NW)ENV02 
ENV02 
49.839891 
-8.301748 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
70.87 
29.11 
JB(NW)ENV03 
ENV03 
49.84083 
-8.10038 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
74.46 
25.52 
JB(NW)ENV06 
ENV06 
49.866479 
-8.139138 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
76.51 
23.49 
JB(NW)ENV08 
ENV08 
49.875291 
-8.084898 
mud and sandy mud 
A5.3 Subtidal Mud 
0.09 
72.56 
27.35 
JB(NW)ENV09 
ENV09 
49.883258 
-8.232186 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
65.77 
34.20 
JB(NW)ENV10 
ENV10 
49.892115 
-8.177943 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
64.69 
35.31 
JB(NW)ENV11 
ENV11 
49.899958 
-8.325303 
mud and sandy mud 
A5.3 Subtidal Mud 
0.12 
65.93 
33.95 
JB(NW)ENV12 
ENV12 
49.908858 
-8.271056 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
63.86 
36.14 
JB(NW)ENV13 
ENV13 
49.90975 
-8.069401 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
73.11 
26.89 
JB(NW)ENV14 
ENV14 
49.917735 
-8.216791 
mud and sandy mud 
A5.3 Subtidal Mud 
0.03 
67.17 
32.80 
JB(NW)ENV15 
ENV15 
49.926584 
-8.162508 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
65.50 
34.50 
JB(NW)ENV16 
ENV16 
49.934445 
-8.309973 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
64.14 
35.86 
JB(NW)ENV17 
ENV17 
49.935409 
-8.108206 
sand and muddy sand 
A5.2 Subtidal Sand 
2.83 
95.20 
1.97 
JB(NW)ENV19 
ENV19 
49.952208 
-8.201379 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
64.70 
35.30 
JB(NW)ENV20 
ENV20 
49.961053 
-8.147056 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
55.70 
44.30 
JB(NW)ENV21 
ENV21 
49.968929 
-8.294624 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
59.23 
40.77 
JB(NW)ENV22 
ENV22 
49.969869 
-8.092713 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
60.92 
39.08 
JB(NW)ENV23 
ENV23 
49.977818 
-8.240296 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
57.91 
42.09 
JB(NW)ENV24 
ENV24 
49.986681 
-8.185951 
sand and muddy sand 
A5.2 Subtidal Sand 
0.00 
91.09 
8.91 
JB(NW)ENV25 
ENV25 
49.995518 
-8.131586 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
60.76 
39.24 
JB(NW)ENV26 
ENV26 
50.003413 
-8.279258 
mud and sandy mud 
A5.3 Subtidal Mud 
0.05 
69.83 
30.11 
JB(NW)ENV27 
ENV27 
50.00433 
-8.077203 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
57.37 
42.63 
JB(NW)ENV29 
ENV29 
49.826853 
-8.100372 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
58.68 
41.32 
JB(NW)ENV30 
ENV30 
49.826988 
-8.062628 
sand and muddy sand 
A5.2 Subtidal Sand 
0.15 
99.85 
0.00 
JB(NW)ENV32 
ENV32 
49.830153 
-8.080048 
mud and sandy mud 
A5.3 Subtidal Mud 
0.03 
67.67 
32.30 
JB(NW)ENV33 
ENV33 
49.831663 
-8.107633 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
70.65 
29.35 
JB(NW)ENV36 
ENV36 
49.83345 
-8.059723 
sand and muddy sand 
A5.2 Subtidal Sand 
0.01 
80.52 
19.46 
JB(NW)ENV37 
ENV37 
49.836615 
-8.077146 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
69.11 
30.87 
Stn No. 
Stn Code 
Latitude 
Longitude 
Sediment Description 
EUNIS Level 3/BSH 
Gravel (%) 
Sand (%) 
Silt/clay (%) 
JB(NW)ENV40 
ENV40 
49.844723 
-8.064076 
mud and sandy mud 
A5.3 Subtidal Mud 
0.00 
64.55 
35.45 
JB(NW)ENV46 
ENV46 
49.905753 
-8.130958 
coarse sediment 
A5.1 Subtidal Coarse Sediment 
13.05 
84.44 
2.50 
JB(NW)ENV48 
ENV48 
49.945588 
-8.33181 
mixed sediments 
A5.4 Subtidal Mixed Sediments 
8.52 
76.04 
15.44 
JB(NW)ENV50 
ENV50 
49.922246 
-8.090859 
mixed sediments 
A5.4 Subtidal Mixed Sediments 
38.54 
52.06 
9.41 
JB(NW)ENV52 
ENV52 
49.942519 
-8.061405 
coarse sediment 
A5.1 Subtidal Coarse Sediment 
20.48 
75.52 
4.00 
JB(NW)ENV54 
ENV54 
49.888545 
-8.107084 
mixed sediments 
A5.4 Subtidal Mixed Sediments 
39.58 
44.54 
15.88 
JB(NW)ENV55 
ENV55 
49.894811 
-8.141331 
sand and muddy sand 
A5.2 Subtidal Sand 
1.96 
97.41 
0.64 
185 
NWJB01 
50.00291 
-8.28246 
mud and sandy mud 
A5.3 Subtidal Mud 
1.81 
61 
37.19 
192 
NWJB02 
49.84015 
-8.30154 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
66.94 
33.03 
190 
NWJB03 
49.87491 
-8.2834 
mud and sandy mud 
A5.3 Subtidal Mud 
0.05 
65.29 
34.66 
193 
NWJB04 
49.84802 
-8.2534 
mud and sandy mud 
A5.3 Subtidal Mud 
0.07 
66.43 
33.5 
182 
NWJB05 
49.99577 
-8.33203 
mud and sandy mud 
A5.3 Subtidal Mud 
0 
51.78 
48.22 
187 
NWJB06 
49.94357 
-8.25406 
mud and sandy mud 
A5.3 Subtidal Mud 
0 
54.95 
45.05 
195 
NWJB07 
49.85772 
-8.19338 
mud and sandy mud 
A5.3 Subtidal Mud 
0.01 
69.37 
30.62 
205 
NWJB08 
50.00451 
-8.07729 
mud and sandy mud 
A5.3 Subtidal Mud 
0.02 
57.36 
42.62 

## Appendix 6. Bsh/Eunis Level 3 Descriptions Derived From Video And Stills

Stn No. 
Stn Code 
Latitude 
Longitude 
Habitat No. 
No. of stills 
Sediment Description 
EUNIS Level 3/BSH 
MNCR Code 
JB(NW)ENV02 
ENV2 
49.83989 
-8.301748 
1 
3 
Mud with burrows and tracks 
A5.3 - Subtidal Mud 
SS.SMu 
JB(NW)ENV03 
ENV3 
49.84083 
-8.10038 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with burrows and tracks and very sparse shell fragments 
JB(NW)ENV08 
ENV8 
49.87529 
-8.084898 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with burrows and tracks and very sparse shell fragments 
JB(NW)ENV09 
ENV9 
49.88325 
-8.232186 
1 
3 
Mud with burrows and tracks  
A5.3 - Subtidal Mud 
SS.SMu 
JB(NW)ENV11 
ENV11 
49.89995 
-8.325303 
1 
3 
Mud with burrows and tracks  
A5.3 - Subtidal Mud 
SS.SMu 
JB(NW)ENV17 
ENV17 
49.93540 
-8.108206 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
Mud with Ditrupa shells 
and fragments of polychaeta tubes 
JB(NW)ENV17 
ENV17a 
49.93540 
-8.108206 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
Mud with  Ditrupa shells and fragments of polychaeta tubes 
JB(NW)ENV19 
ENV19 
49.95220 
-8.201379 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with burrows and tracks, and occasional ripples 
JB(NW)ENV24 
ENV24 
49.98668 
-8.185951 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with  patches of coarse sediment, dense Ditrupa shells, burrows and occasional ripples 
JB(NW)ENV41 
ENV41 
49.89565 
-8.12532 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with fragments of polychaeta tubes, empty shells and patches of coarse sediment and gravel 
JB(NW)ENV51 
ENV51 
49.94817 
-8.06433 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with fragments of polychaeta tubes, empty shells and patches of coarse sediment and gravel 
Stn No. 
Stn Code 
Latitude 
Longitude 
Habitat No. 
No. of stills 
Sediment Description 
EUNIS Level 3/BSH 
MNCR Code 
JB(NW)ENV51 
ENV51a 
49.93133 
-8.0645 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with fragments of 
polychaeta tubes, empty shells and patches of coarse sediment and gravel 
JB(NW)ENV55 
ENV55 
49.89481 
-8.141331 
1 
3 
A5.3 - Subtidal Mud 
SS.SMu 
Mud with few fragments of polychaeta tubes and empty shells 
183 
NWJB_05 
49.9958 
-8.33202 
1 
16 
Fine mud with Burrowing Megafauna Communities 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
 
201_S1 
NWJB_CS01 
49.9248 
-8.10467 
1 
7 
Sparse fauna on circalittoral  fine mud 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
201_S2 
NWJB_CS01 
49.92548 
-8.10425 
2 
3 
Sparse fauna on pebbly circalittoral fine mud 
A5.4 - Subtidal Mixed Sediments 
SS.SMx.CMx 
201_S3 
NWJB_CS01 
49.91917 
-8.10404 
1 
3 
Sparse fauna on 
circalittoral  fine mud 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
203 
NWJB_CS02 
49.97996 
-8.0699 
1 
19 
Fine mud with Burrowing Megafauna Communities 
A5.3 - Subtidal Mud 
SS.SMu.CFiMu 
199_S1 
NWJB_CS06 
49.82597 
-8.06545 
1 
11 
Sparse fauna on circalittoral  sandy mud 
A5.3 - Subtidal Mud 
SS.SMu.CSaMu 
199_S2 
NWJB_CS06 
49.82661 
-8.06378 
2 
4 
A5.4 - Subtidal Mixed Sediments 
SS.SMx.CMx.ClloMx 
Cerianthus lloydii and other burrowing anemones in circalittoral muddy mixed sediments  
199_S3 
NWJB_CS06 
49.82672 
-8.065 
1 
16 
Sparse fauna on circalittoral sandy mud 
A5.3 - Subtidal Mud 
SS.SMu.CSaMu 

## Appendix 7.  Example Images From Survey Of Broadscale Habitats

| Broadscale Habitats     | Description          |
|-------------------------|----------------------|
| A5.1: Sublittoral       |                      |
| coarse sediment         |                      |
| Coarse sediments        |                      |
| including coarse sand,  |                      |
| gravel, pebbles,        |                      |
| shingle and cobbles     |                      |
| which are often         |                      |
| unstable due to tidal   |                      |
| currents and/or wave    |                      |
| action.                 |                      |
| A5.2: Sublittoral sand  | Clean medium to fine |
| sands or non-cohesive   |                      |
| slightly muddy sands    |                      |
| on open coasts,         |                      |
| offshore or in          |                      |
| estuaries and marine    |                      |
| inlets.                 |                      |
| A5.3 Subtidal Mud       | Sublittoral mud and  |
| cohesive sandy mud      |                      |
| extending from the      |                      |
| extreme lower shore to  |                      |
| offshore, circalittoral |                      |
| habitats.               |                      |
| A5.4: Sublittoral mixed |                      |
| sediments               |                      |
| Sublittoral mixed       |                      |
| (heterogeneous)         |                      |
| sediments found from    |                      |
| the extreme low water   |                      |
| mark to deep offshore   |                      |
| circalittoral habitats. |                      |
No Image Available 
No Image Available 

## Appendix 8. Example Images From Survey Of Habitat Foci

| Habitat FOCI          |
|-----------------------|
| Subtidal Sands and    |
| Gravel                |
| Sand and gravel       |
| seabeds widespread    |
| around the UK         |
| Sea-pen and Burrowing |
| Megafauna             |
| Communities           |
| Areas of stable muddy |
| seabed, where         |
| animals burrow below  |
| and sea-pens protrude |
| from the surface      |
| Mud Habitats in Deep  |
| Water                 |
| Mud habitats in deep  |
| water (circalittoral  |
| muds) occur below     |
| 20-30 m in many areas |
| of the UK's marine    |
| environment,          |
| including marine      |
| inlets such as sea    |
| lochs.                |
No Image Available 

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