# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 161

# Report Period 1St - 31St January 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |

## Table 1. Monthly Totals For January 2020

| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| January                 |                          |          |
| 2020                    |                          |          |
| Submissions             | 343                      | 343      |
| Samples                 | Total (%)                | 31274    |
| England                 | 24247                    | 77.53 %  |
| Wales                   | 5757                     | 18.41 %  |
| Scotland                | 1270                     | 4.06 %   |
| Parallel Tests          | PAR-CUL-N                | 8782     |
| PAR-CUL-P               | 2044                     | 6.54 %   |
| PAR-HERD-S              | 22                       | 0.07 %   |
| PAR-NEW-IN              | 13601                    | 43.49 %  |
| PAR-OTHER               | 596                      | 1.91 %   |
| PAR-PERSIS              | 5068                     | 16.21 %  |
| PAR-RAPID               | 61                       | 0.20 %   |
| Total(% of all samples) | 30174                    | 96.48 %  |
| Serial/Extended Tests   | SER-FLEXI                | 1100     |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 1100                     | 3.52 %   |
| Total                   | 31274                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2068     |
| England                 | 1546                     | 4.94 %   |
| Wales                   | 448                      | 1.43 %   |
| Scotland                | 74                       | 0.24 %   |
| Resamples               | Total (% of all samples) | 1611     |
| England                 | 1174                     | 3.75 %   |
| Wales                   | 375                      | 1.20 %   |
| Scotland                | 62                       | 0.20 %   |
| Rejects                 | Total (% of all samples) | 497      |
| England                 | 487                      | 1.56 %   |
| Wales                   | 10                       | 0.03 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   8782 | 28.1 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   2044 | 6.5 %    |
| PAR-HERD-S  |     22 | 0.1 %    |
| PAR-NEW-IN  |  13601 | 43.5 %   |
| PAR-OTHER   |    596 | 1.9 %    |
| PAR-PERSIS  |   5068 | 16.2 %   |
| PAR-RAPID   |     61 | 0.2 %    |
| SER-FLEXI   |   1100 | 3.5 %    |
| Total       |  31274 | 100.00 % |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 8              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 6              |
| SER-FLEXI       | 2              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| SER-FLEXI       | 2              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-PERSIS     |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2A.  Summary By County For January 2020 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-CUL-N      |
| PAR-NEW-IN      | 13             |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Gr Manchester  |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| England         |                |
| 216             | 24247          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 7              |
| PAR-RAPID       | 7              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 3              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 3              |
| SER-FLEXI       | 1              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 8              |
| PAR-PERSIS      | 11             |
| PAR-RAPID       | 7              |
| SER-FLEXI       | 2              |
| Powys           | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2A.  Summary By County For January 2020 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-NEW-IN              | 7              |
| PAR-PERSIS              | 8              |
| SER-FLEXI               | 4              |
| S Wales                 | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | SER-FLEXI      |
| Wales - Low TB          | Gwynedd        |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 117                     | 5757           |
| Scotland - Scotland     | Ayrshire       |
| Dumfries & G            | PAR-NEW-IN     |
| Kirkcudbright           | PAR-NEW-IN     |
| Wigtown                 | PAR-NEW-IN     |
| Scotland                |                |
| 10                      | 1270           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 8              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 6              |
| SER-FLEXI       | 2              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| SER-FLEXI       | 2              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-PERSIS     |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-CUL-N      |
| PAR-NEW-IN      | 13             |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Gr Manchester  |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| England         |                |
| 216             | 24247          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 7              |
| PAR-RAPID       | 7              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 3              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 3              |
| SER-FLEXI       | 1              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 8              |
| PAR-PERSIS      | 11             |
| PAR-RAPID       | 7              |
| SER-FLEXI       | 2              |
| Powys           | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-NEW-IN              | 7              |
| PAR-PERSIS              | 8              |
| SER-FLEXI               | 4              |
| S Wales                 | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | SER-FLEXI      |
| Wales - Low TB          | Gwynedd        |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 117                     | 5757           |
| Scotland - Scotland     | Ayrshire       |
| Dumfries & G            | PAR-NEW-IN     |
| Kirkcudbright           | PAR-NEW-IN     |
| Wigtown                 | PAR-NEW-IN     |
| Scotland                |                |
| 10                      | 1270           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol January 2020

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 8            |
| Total           | 216          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 10           |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 117          |
| GB              | Parallel     |
| Serial/Extended | 18           |
| Total           | 343          |
| 216.00          |              |
| 117.00          |              |
| 10.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 8            |
| Total           | 216          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 10           |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 117          |
| GB              | Parallel     |
| Serial/Extended | 18           |
| Total           | 343          |
| 216.00          |              |
| 117.00          |              |
| 10.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (January 2020)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3 %
43
3.9 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
126
13601
43.5%
400
2.9%
12157
89.4%
893
6.6 %
689
5.1 %
355
2.6 %
Parallel - new infection cull area
85
8782
28.1%
404
4.6%
7762
88.4%
603
6.9 %
481
5.5 %
135
1.5 %
Parallel - Other
13
596
1.9%
49
8.2%
524
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
58
5068
16.2%
325
6.4%
4469
88.2%
339
6.7 %
267
5.3 %
7
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
Parallel - Rapid Testing Two-times IRs
24
61
0.2%
19
31.1%
40
65.6%
2
3.3 %
2
3.3 %
0
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6 %
1611
5.2 %
497
1.6 %
England - HRA
Extended Flexible IFN-GAMMA test
8
854
2.7%
50
5.9%
770
90.2%
46
5.4 %
34
4.0 %
0
Parallel New Infection
2
244
0.8%
10
4.1%
204
83.6%
37
15.2 %
29
11.9 %
1
0.4 %
Parallel - new infection cull area
83
8400
26.9%
386
4.6%
7421
88.3%
560
6.7 %
458
5.5 %
135
1.6 %
Parallel Persistent Infection
14
2466
7.9%
122
4.9%
2244
91.0%
138
5.6 %
98
4.0 %
2
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
England - HRA
120
14008
44.8%
678
4.8%
12468
89.0%
920
6.6 %
724
5.2 %
138
1.0 %
England - Edge
Parallel New Infection
90
9650
30.9%
310
3.2%
8675
89.9%
575
6.0 %
420
4.4 %
245
2.5 %
Parallel - new infection cull area
2
382
1.2%
18
4.7%
341
89.3%
43
11.3 %
23
6.0 %
0
England - Edge
92
10032
32.1%
328
3.3%
9016
89.9%
618
6.2 %
443
4.4 %
245
2.4 %
England - LRA
Parallel New Infection
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
England - LRA
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
242
0.8%
7
2.9%
226
93.4%
12
5.0 %
9
3.7 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
7
873
2.8%
30
3.4%
735
84.2%
113
12.9 %
103
11.8 %
5
0.6 %
Parallel - Other
12
595
1.9%
49
8.2%
523
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
38
2413
7.7%
172
7.1%
2073
85.9%
195
8.1 %
163
6.8 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
20
57
0.2%
18
31.6%
37
64.9%
2
3.5 %
2
3.5 %
0
Wales - High TB
91
4202
13.4%
278
6.6%
3613
86.0%
356
8.5 %
301
7.2 %
10
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
11
986
3.2%
21
2.1%
909
92.2%
66
6.7 %
56
5.7 %
0
Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
6
189
0.6%
31
16.4%
152
80.4%
6
3.2 %
6
3.2 %
0
Parallel - Rapid Testing Two-times IRs
3
3
0.0%
1
33.3%
2
66.7%
0
0
0
Wales - Intermediate TB
23
1183
3.8%
54
4.6%
1067
90.2%
72
6.1 %
62
5.2 %
0
Wales - Low TB
Parallel New Infection
2
371
1.2%
3
0.8%
356
96.0%
20
5.4 %
12
3.2 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
3
372
1.2%
3
0.8%
357
96.0%
20
5.4 %
12
3.2 %
0
Scotland - Scotland
Parallel New Infection
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
Scotland - Scotland
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
18
1100
3.5%
58
5.3%
999
90.8%
58
5.3 %
43
3.9 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
126
13601
43.5%
400
2.9%
12157
89.4%
893
6.6 %
689
5.1 %
355
2.6 %
Parallel - new infection cull area
85
8782
28.1%
404
4.6%
7762
88.4%
603
6.9 %
481
5.5 %
135
1.5 %
Parallel - Other
13
596
1.9%
49
8.2%
524
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
58
5068
16.2%
325
6.4%
4469
88.2%
339
6.7 %
267
5.3 %
7
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
Parallel - Rapid Testing Two-times IRs
24
61
0.2%
19
31.1%
40
65.6%
2
3.3 %
2
3.3 %
0
Total
343
31274
100.0%
1367
4.4%
27799
88.9%
2068
6.6 %
1611
5.2 %
497
1.6 %
England - HRA
Extended Flexible IFN-GAMMA test
8
854
2.7%
50
5.9%
770
90.2%
46
5.4 %
34
4.0 %
0
Parallel New Infection
2
244
0.8%
10
4.1%
204
83.6%
37
15.2 %
29
11.9 %
1
0.4 %
Parallel - new infection cull area
83
8400
26.9%
386
4.6%
7421
88.3%
560
6.7 %
458
5.5 %
135
1.6 %
Parallel Persistent Infection
14
2466
7.9%
122
4.9%
2244
91.0%
138
5.6 %
98
4.0 %
2
0.1 %
Parallel - persistent infection cull area
13
2044
6.5%
110
5.4%
1829
89.5%
139
6.8 %
105
5.1 %
0
England - Edge
Parallel New Infection
90
9650
30.9%
310
3.2%
8675
89.9%
575
6.0 %
420
4.4 %
245
2.5 %
Parallel - new infection cull area
2
382
1.2%
18
4.7%
341
89.3%
43
11.3 %
23
6.0 %
0
England - LRA
Parallel New Infection
4
207
0.7%
3
1.4%
93
44.9%
8
3.9 %
7
3.4 %
104
50.2 %
England
216
24247
77.5%
1009
4.2%
21577
89.0%
1546
6.4 %
1174
4.8 %
487
2.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
8
242
0.8%
7
2.9%
226
93.4%
12
5.0 %
9
3.7 %
0
Parallel - Herd or Group Slaughter
6
22
0.1%
2
9.1%
19
86.4%
3
13.6 %
1
4.5 %
0
Parallel New Infection
7
873
2.8%
30
3.4%
735
84.2%
113
12.9 %
103
11.8 %
5
0.6 %
Parallel - Other
12
595
1.9%
49
8.2%
523
87.9%
31
5.2 %
23
3.9 %
0
Parallel Persistent Infection
38
2413
7.7%
172
7.1%
2073
85.9%
195
8.1 %
163
6.8 %
5
0.2 %
Parallel - Rapid Testing Two-times IRs
20
57
0.2%
18
31.6%
37
64.9%
2
3.5 %
2
3.5 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
4
0.0%
1
25.0%
3
75.0%
0
0
0
Parallel New Infection
11
986
3.2%
21
2.1%
909
92.2%
66
6.7 %
56
5.7 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel - Other
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel Persistent Infection
6
189
0.6%
31
16.4%
152
80.4%
6
3.2 %
6
3.2 %
0
Parallel - Rapid Testing Two-times IRs
3
3
0.0%
1
33.3%
2
66.7%
0
0
0
Wales - Low TB
Parallel New Infection
2
371
1.2%
3
0.8%
356
96.0%
20
5.4 %
12
3.2 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
117
5757
18.4%
335
5.8%
5037
87.5%
448
7.8 %
375
6.5 %
10
0.2 %
Scotland - Scotland
Parallel New Infection
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
Scotland
10
1270
4.1%
23
1.8%
1185
93.3%
74
5.8 %
62
4.9 %
0
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2019         | February |
| March        | 315      |
| April        | 269      |
| May          | 364      |
| June         | 287      |
| July         | 316      |
| August       | 262      |
| September    | 245      |
| October      | 298      |
| November     | 272      |
| December     | 234      |
| 2020         | January  |
| Totals (last |          |
| 12 months)   |          |
| 3525         | 335727   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2108
267436
1340
10146
506
0
0
0
0
1016
9
8
50
1
8438
7
64
0
1
Scotland
32
3963
16
113
1
0
0
0
0
13
0
1
2
0
90
0
5
0
0
Wales
1385
64328
705
3139
139
0
0
0
0
115
6
2
19
4
2187
70
533
2
19
Totals
3525
335727
2061
13398
646
0
0
0
0
1144
15
11
71
5
10715
77
602
2
20
|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Ayrshire      | 6.01 %   | 7.83 %                 |
| Berks         | 6.19 %   | 14.43 %                |
| Bucks         | 17.91 %  | 18.58 %                |
| Carms         | 2.57 %   | 3.57 %                 |
| Ceredigion    | 4.11 %   | 5.07 %                 |
| Cheshire      | 3.16 %   | 4.56 %                 |
| Corn & Scilly | 3.47 %   | 4.91 %                 |
| Derbyshire    | 4.79 %   | 6.46 %                 |
| Devon         | 5.81 %   | 6.98 %                 |
| Dorset        | 3.62 %   | 5.34 %                 |
| Dumfries & G  | 4.04 %   | 4.57 %                 |
| E Sussex      | 2.43 %   | 3.32 %                 |
| Gloucs        | 7.62 %   | 9.70 %                 |
| Gr Manchester | 3.85 %   | 3.85 %                 |
| Gwent         | 2.23 %   | 3.47 %                 |
| Gwynedd       | 11.11 %  | 11.11 %                |
| Hampshire     | 6.28 %   | 10.14 %                |
| Heref         | 7.43 %   | 8.72 %                 |
| Kirkcudbright |          |                        |
| Leics & Rut   | 4.56 %   | 5.74 %                 |
| NE Wales      | 4.90 %   | 6.00 %                 |
| Norfolk       | 15.00 %  | 15.00 %                |
| Northants     | 6.04 %   | 7.38 %                 |
| Notts         | 1.33 %   | 2.67 %                 |
|                 | N Yorks   | 0.71 %   |
|-----------------|-----------|----------|
| Oxon            | 4.84 %    | 6.98 %   |
| Pembs           | 6.50 %    | 7.73 %   |
| Powys           | 13.13 %   | 14.75 %  |
| S Glamorgan     |           |          |
| Shropshire      | 5.30 %    | 6.62 %   |
| Somerset excl N | 4.51 %    | 6.17 %   |
| S Wales         | 1.90 %    | 5.71 %   |
| Warks           | 2.13 %    | 3.96 %   |
| W Glamorgan     | 100.00 %  | 100.00 % |
| Wigtown         | 5.39 %    | 6.06 %   |
| Wiltshire   | 5.51 %   | 7.38 %   |
|-------------|----------|----------|
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23736
1009
4.3%
24247
1009
4.2%
Wales
5668
335
5.9%
5757
335
5.8%
Scotland
1225
23
1.9%
1270
23
1.8%
GB
30629
1367
4.5%
31274
1367
4.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
6
0
0
Dorset
PRV-SE
1
1
0
0
Staffordshire
PRV-SE
1
24
0
0
Worcestershire
PRV-SP
1
1
0
0
Total
4
32
0
Isle of Man
SER-IOM
6
14
0
0
| % Positive     | * Test type   |
|----------------|---------------|
| Num            |               |
| Submissions    |               |
| Num            |               |
| Samples        |               |
| Num            |               |
| Positives      |               |
| Private        |               |
| Devon          | PRV-SP        |
| Dorset         | PRV-SE        |
| Staffordshire  | PRV-SE        |
| Worcestershire | PRV-SP        |
| Total          |               |
| 4              |               |
| 32             |               |
| 0              |               |
| Isle of Man    | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        1 |                17 | 2                    | 11.8%                             |
| Devon            | 1               |      630 |                42 | 6.7%                 |                                   |
| North East Wales | 3               |       43 |                 6 | 14.0%                |                                   |
| Pembrokeshire    | 3               |       68 |                 9 | 13.2%                |                                   |
| South Wales      | 1               |       35 |                 3 | 8.6%                 |                                   |
| TC0077           | 9               |      793 |                62 | 7.8 %                |                                   |
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        1 |                17 | 2                    | 11.8%                             |
| Devon            | 1               |      630 |                42 | 6.7%                 |                                   |
| North East Wales | 3               |       43 |                 6 | 14.0%                |                                   |
| Pembrokeshire    | 3               |       68 |                 9 | 13.2%                |                                   |
| South Wales      | 1               |       35 |                 3 | 8.6%                 |                                   |
| TC0077           | 9               |      793 |                62 | 7.8 %                |                                   |