# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 148

# Report Period 1St - 31St December 2018

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2018               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| December                |                          |          |
| 2018                    |                          |          |
| Submissions             | 228                      | 2669     |
| Samples                 | Total (%)                | 21554    |
| England                 | 15274                    | 70.86 %  |
| Wales                   | 6280                     | 29.14 %  |
| Scotland                | 2241                     | 0.88 %   |
| Parallel Tests          | PAR-CUL-N                | 2693     |
| PAR-CUL-P               | 424                      | 1.97 %   |
| PAR-HERD-S              | 1631                     | 7.57 %   |
| PAR-NEW-IN              | 11486                    | 53.29 %  |
| PAR-OTHER               | 1128                     | 5.23 %   |
| PAR-PERSIS              | 3814                     | 17.70 %  |
| PAR-RAPID               | 63                       | 0.29 %   |
| Total(% of all samples) | 21239                    | 98.54 %  |
| Serial/Extended Tests   | SER-FLEXI                | 315      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 315                      | 1.46 %   |
| Total                   | 21554                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1642     |
| England                 | 1192                     | 5.53 %   |
| Wales                   | 450                      | 2.09 %   |
| Scotland                | 201                      | 0.08 %   |
| Resamples               | Total (% of all samples) | 1211     |
| England                 | 918                      | 4.26 %   |
| Wales                   | 293                      | 1.36 %   |
| Scotland                | 139                      | 0.05 %   |
| Rejects                 | Total (% of all samples) | 131      |
| England                 | 110                      | 0.51 %   |
| Wales                   | 21                       | 0.10 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   2693 | 12.5 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    424 | 2.0 %    |
| PAR-HERD-S  |   1631 | 7.6 %    |
| PAR-NEW-IN  |  11486 | 53.3 %   |
| PAR-OTHER   |   1128 | 5.2 %    |
| PAR-PERSIS  |   3814 | 17.7 %   |
| PAR-RAPID   |     63 | 0.3 %    |
| SER-FLEXI   |    315 | 1.5 %    |
| Total       |  21554 | 100.00 % |
Breakdown
of Samples
submitted
by County
| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Corn & Scilly  |
| PAR-NEW-IN     | 1              |
| PAR-PERSIS     | 1              |
| Devon          | PAR-CUL-N      |
| PAR-CUL-P      | 1              |
| PAR-PERSIS     | 3              |
| Dorset         | PAR-CUL-N      |
| Gloucs         | PAR-CUL-N      |
| Heref          | PAR-CUL-P      |
| PAR-OTHER      | 1              |
| PAR-PERSIS     | 2              |
| Shropshire     | PAR-PERSIS     |
| Somerset       | PAR-CUL-N      |
| England - Edge | Berks          |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| Hampshire      | PAR-NEW-IN     |
| Leics & Rut    | PAR-NEW-IN     |
| PAR-OTHER      | 2              |
| Northants      | PAR-NEW-IN     |
| Oxon           | PAR-NEW-IN     |
| Warks          | PAR-NEW-IN     |
| England - LRA  | Cambs          |
| Cumbria        | PAR-NEW-IN     |
| Isle of wight  | PAR-NEW-IN     |
| Lincs          | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For December 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| England                 |                |
| 122                     | 15274          |
| Wales - High TB         | Carms          |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 9              |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 5              |
| Ceredigion              | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| Gwent                   | PAR-PERSIS     |
| PAR-RAPID               | 4              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 3              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 2              |
| PAR-PERSIS              | 2              |
| S Wales                 | PAR-OTHER      |
| PAR-RAPID               | 1              |
| W Glamorgan             | PAR-OTHER      |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-HERD-S     |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For December 2018 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| PAR-RAPID      | 1              |
| SER-FLEXI      | 2              |
| Shropshire     | PAR-NEW-IN     |
| W Glamorgan    | PAR-OTHER      |
| Wales - Low TB | Gwynedd        |
| NE Wales       | PAR-NEW-IN     |
| Wales          |                |
| 106            | 6280           |
| Grand Total    | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-PERSIS    | 8              |
| SER-FLEXI     | 2              |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 3              |
| PAR-PERSIS    | 23             |
| SER-FLEXI     | 9              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 19             |
| PAR-NEW-IN    | 9              |
| PAR-OTHER     | 6              |
| PAR-PERSIS    | 38             |
| SER-FLEXI     | 14             |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 18             |
| SER-FLEXI     | 1              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 9              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 5              |
| SER-FLEXI     | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| Heref          | PAR-CUL-N      |
| PAR-CUL-P      | 4              |
| PAR-NEW-IN     | 3              |
| PAR-OTHER      | 1              |
| PAR-PERSIS     | 6              |
| SER-FLEXI      | 2              |
| SER-FRAUD      | 2              |
| Shropshire     | PAR-NEW-IN     |
| PAR-OTHER      | 8              |
| PAR-PERSIS     | 16             |
| Somerset       | PAR-CUL-N      |
| PAR-CUL-P      | 4              |
| PAR-NEW-IN     | 1              |
| PAR-OTHER      | 6              |
| PAR-PERSIS     | 21             |
| Staffs         | PAR-PERSIS     |
| SER-FLEXI      | 4              |
| Wiltshire      | PAR-NEW-IN     |
| PAR-OTHER      | 5              |
| PAR-PERSIS     | 12             |
| SER-FLEXI      | 6              |
| SER-FRAUD      | 2              |
| W Midlands     | PAR-PERSIS     |
| SER-FRAUD      | 3              |
| England - Edge | Berks          |
| PAR-PERSIS     | 6              |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| PAR-PERSIS    | 10             |
| Derbyshire    | PAR-NEW-IN     |
| E Sussex      | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| Hampshire     | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 1              |
| SER-NSR       | 3              |
| Leics & Rut   | PAR-NEW-IN     |
| PAR-OTHER     | 4              |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| Oxon          | PAR-NEW-IN     |
| PAR-PERSIS    | 14             |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| England - LRA | Beds           |
| Cambs         | PAR-NEW-IN     |
| Cumbria       | PAR-NEW-IN     |
| PAR-OTHER     | 3              |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| Humberside    | PAR-NEW-IN     |
| Isle of wight | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Lincs           | PAR-NEW-IN     |
| Merseyside      | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England         |                |
| 1469            | 193638         |
| Wales - High TB | Carms          |
| PAR-OTHER       | 12             |
| PAR-PERSIS      | 61             |
| PAR-RAPID       | 97             |
| SER-FLEXI       | 17             |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 18             |
| PAR-RAPID       | 32             |
| SER-FLEXI       | 11             |
| Gwent           | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 40             |
| PAR-RAPID       | 41             |
| SER-FLEXI       | 6              |
| Pembrokshire    | PAR-OTHER      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 6              |
| PAR-PERSIS      | 91             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 143            |
| SER-FLEXI               | 32             |
| SER-FRAUD               | 2              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 24             |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 50             |
| PAR-RAPID               | 35             |
| SER-FLEXI               | 6              |
| S Wales                 | PAR-OTHER      |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 4              |
| W Glamorgan             | PAR-OTHER      |
| PAR-RAPID               | 2              |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 5              |
| Ceredigion              | PAR-HERD-S     |
| PAR-NEW-IN              | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 21             |
| SER-FLEXI               | 7              |
| Mid glamorgan           | PAR-PERSIS     |
| NE Wales                | PAR-HERD-S     |
| PAR-NEW-IN              | 133            |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 11             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| SER-FLEXI           | 11             |
| Powys               | PAR-HERD-S     |
| PAR-NEW-IN          | 8              |
| PAR-RAPID           | 3              |
| Shropshire          | PAR-NEW-IN     |
| W Glamorgan         | PAR-OTHER      |
| SER-FLEXI           | 2              |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 3              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1169                | 57607          |
| Scotland - Scotland | Aberdeenshire  |
| Argyll & Bute       | PAR-NEW-IN     |
| SER-FLEXI           | 1              |
| Ayrshire            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| Perthshire          | PAR-NEW-IN     |
| West lothian        | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 31                  | 2241           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol December 2018

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 122          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 13           |
| Total           | 106          |
| GB              | Parallel     |
| Serial/Extended | 13           |
| Total           | 228          |
| 122.00          |              |
| 106.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2018)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 55           |
| Total           | 1469 193638  |
| Scotland        | Parallel     |
| Serial/Extended | 1            |
| Total           | 31           |
| Wales           | Parallel     |
| Serial/Extended | 96           |
| Total           | 1169         |
| GB              | Parallel     |
| Serial/Extended | 152          |
| Total           | 2669 253486  |

####### #######
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
13
315
1.5%
48
15.2%
256
81.3%
13
4.1 %
9
2.9 %
2
0.6 %
Parallel - Herd or Group Slaughter
21
1631
7.6%
114
7.0%
1437
88.1%
111
6.8 %
72
4.4 %
8
0.5 %
Parallel New Infection
98
11486
53.3%
535
4.7%
10118
88.1%
955
8.3 %
727
6.3 %
106
0.9 %
Parallel - new infection cull area
28
2693
12.5%
144
5.3%
2431
90.3%
157
5.8 %
116
4.3 %
2
0.1 %
Parallel - Other
15
1128
5.2%
95
8.4%
953
84.5%
106
9.4 %
79
7.0 %
1
0.1 %
Parallel Persistent Infection
28
3814
17.7%
260
6.8%
3379
88.6%
245
6.4 %
163
4.3 %
12
0.3 %
Parallel - persistent infection cull area
3
424
2.0%
31
7.3%
350
82.5%
51
12.0 %
43
10.1 %
0
Parallel - Rapid Testing Two-times IRs
22
63
0.3%
14
22.2%
47
74.6%
4
6.3 %
2
3.2 %
0
Total
228
21554
100.0%
1241
5.8%
18971
88.0%
1642
7.6 %
1211
5.6 %
131
0.6 %
England - HRA
Parallel New Infection
1
183
0.8%
6
3.3%
154
84.2%
28
15.3 %
23
12.6 %
0
Parallel - new infection cull area
28
2693
12.5%
144
5.3%
2431
90.3%
157
5.8 %
116
4.3 %
2
0.1 %
Parallel - Other
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Parallel Persistent Infection
9
1793
8.3%
138
7.7%
1580
88.1%
101
5.6 %
71
4.0 %
4
0.2 %
Parallel - persistent infection cull area
3
424
2.0%
31
7.3%
350
82.5%
51
12.0 %
43
10.1 %
0
England - HRA
42
5098
23.7%
319
6.3%
4520
88.7%
337
6.6 %
253
5.0 %
6
0.1 %
England - Edge
Parallel New Infection
72
9398
43.6%
474
5.0%
8233
87.6%
765
8.1 %
589
6.3 %
102
1.1 %
Parallel - Other
2
24
0.1%
3
12.5%
19
79.2%
1
4.2 %
1
4.2 %
1
4.2 %
England - Edge
74
9422
43.7%
477
5.1%
8252
87.6%
766
8.1 %
590
6.3 %
103
1.1 %
England - LRA
Parallel New Infection
6
754
3.5%
28
3.7%
650
86.2%
89
11.8 %
75
9.9 %
1
0.1 %
England - LRA
6
754
3.5%
28
3.7%
650
86.2%
89
11.8 %
75
9.9 %
1
0.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
9
267
1.2%
41
15.4%
220
82.4%
8
3.0 %
4
1.5 %
2
0.7 %
Parallel - Herd or Group Slaughter
17
1247
5.8%
80
6.4%
1109
88.9%
77
6.2 %
51
4.1 %
7
0.6 %
Parallel New Infection
2
91
0.4%
2
2.2%
87
95.6%
3
3.3 %
2
2.2 %
0
Parallel - Other
9
486
2.3%
25
5.1%
418
86.0%
60
12.3 %
43
8.8 %
0
Parallel Persistent Infection
18
1913
8.9%
119
6.2%
1700
88.9%
130
6.8 %
86
4.5 %
8
0.4 %
Parallel - Rapid Testing Two-times IRs
20
61
0.3%
13
21.3%
46
75.4%
4
6.6 %
2
3.3 %
0
Wales - High TB
75
4065
18.9%
280
6.9%
3580
88.1%
282
6.9 %
188
4.6 %
17
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
48
0.2%
7
14.6%
36
75.0%
5
10.4 %
5
10.4 %
0
Parallel - Herd or Group Slaughter
4
384
1.8%
34
8.9%
328
85.4%
34
8.9 %
21
5.5 %
1
0.3 %
Parallel New Infection
7
297
1.4%
8
2.7%
273
91.9%
20
6.7 %
14
4.7 %
2
0.7 %
Parallel - Other
3
613
2.8%
67
10.9%
511
83.4%
45
7.3 %
35
5.7 %
0
Parallel Persistent Infection
1
108
0.5%
3
2.8%
99
91.7%
14
13.0 %
6
5.6 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
1
50.0%
1
50.0%
0
0
0
Wales - Intermediate TB
21
1452
6.7%
120
8.3%
1248
86.0%
118
8.1 %
81
5.6 %
3
0.2 %
Wales - Low TB
Parallel New Infection
10
763
3.5%
17
2.2%
721
94.5%
50
6.6 %
24
3.1 %
1
0.1 %
Wales - Low TB
10
763
3.5%
17
2.2%
721
94.5%
50
6.6 %
24
3.1 %
1
0.1 %

## Table 4B.  Summary Of Ifn Gamma Results By Submission Reason  (Total 2018)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
138
9178
3.6%
735
8.0%
7840
85.4%
778
8.5 %
515
5.6 %
88
1.0 %
Parallel - Herd or Group Slaughter
127
8132
3.2%
405
5.0%
6830
84.0%
731
9.0 %
516
6.3 %
381
4.7 %
Parallel New Infection
1142 131838
52.0%
5144
3.9%
118042
89.5%
11297
8.6 %
7823
5.9 %
829
0.6 %
Parallel - new infection cull area
266
31400
12.4%
1754
5.6%
27793
88.5%
2542
8.1 %
1665
5.3 %
188
0.6 %
Parallel - Other
82
5134
2.0%
422
8.2%
4311
84.0%
395
7.7 %
273
5.3 %
128
2.5 %
Parallel Persistent Infection
464
59225
23.4%
3417
5.8%
51980
87.8%
4503
7.6 %
3199
5.4 %
629
1.1 %
Parallel - persistent infection cull area
38
6710
2.6%
383
5.7%
5891
87.8%
501
7.5 %
336
5.0 %
100
1.5 %
Parallel - Rapid Testing Two-times IRs
398
1570
0.6%
352
22.4%
1112
70.8%
102
6.5 %
75
4.8 %
31
2.0 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
2669 253486
100.0%
12670
5.0%
224032
88.4%
20862
8.2 %
14410
5.7 %
2374
0.9 %
England - HRA
Extended Flexible IFN-GAMMA test
43
6550
2.6%
517
7.9%
5699
87.0%
522
8.0 %
321
4.9 %
13
0.2 %
Parallel New Infection
23
2925
1.2%
164
5.6%
2560
87.5%
276
9.4 %
189
6.5 %
12
0.4 %
Parallel - new infection cull area
266
31400
12.4%
1754
5.6%
27793
88.5%
2542
8.1 %
1665
5.3 %
188
0.6 %
Parallel - Other
34
3158
1.2%
206
6.5%
2696
85.4%
210
6.6 %
130
4.1 %
126
4.0 %
Parallel Persistent Infection
151
31312
12.4%
1871
6.0%
27880
89.0%
2167
6.9 %
1496
4.8 %
65
0.2 %
Parallel - persistent infection cull area
38
6710
2.6%
383
5.7%
5891
87.8%
501
7.5 %
336
5.0 %
100
1.5 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
759
93689
37.0%
3948
4.2%
83824
89.5%
8093
8.6 %
5582
6.0 %
335
0.4 %
Parallel - Other
6
53
0.0%
4
7.5%
46
86.8%
3
5.7 %
2
3.8 %
1
1.9 %
Parallel Persistent Infection
35
4577
1.8%
253
5.5%
3903
85.3%
389
8.5 %
319
7.0 %
102
2.2 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
96
12162
4.8%
470
3.9%
10701
88.0%
1142
9.4 %
787
6.5 %
204
1.7 %
Parallel - Other
3
276
0.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
Parallel Persistent Infection
3
627
0.2%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
1469 193638
76.4%
9620
5.0%
171967
88.8%
15940
8.2 %
10905
5.6 %
1146
0.6 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2018) Cont.

Wales - High TB
Extended Flexible IFN-GAMMA test
72
2155
0.9%
186
8.6%
1733
80.4%
219
10.2 %
161
7.5 %
75
3.5 %
Parallel - Herd or Group Slaughter
112
6729
2.7%
345
5.1%
5692
84.6%
460
6.8 %
314
4.7 %
378
5.6 %
Parallel New Infection
26
960
0.4%
23
2.4%
897
93.4%
55
5.7 %
38
4.0 %
2
0.2 %
Parallel - Other
30
787
0.3%
54
6.9%
680
86.4%
72
9.1 %
53
6.7 %
0
Parallel Persistent Infection
263
21940
8.7%
1198
5.5%
19002
86.6%
1810
8.2 %
1278
5.8 %
462
2.1 %
Parallel - Rapid Testing Two-times IRs
354
1398
0.6%
315
22.5%
985
70.5%
93
6.7 %
68
4.9 %
30
2.1 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
20
417
0.2%
31
7.4%
355
85.1%
34
8.2 %
31
7.4 %
0
Parallel - Herd or Group Slaughter
15
1403
0.6%
60
4.3%
1138
81.1%
271
19.3 %
202
14.4 %
3
0.2 %
Parallel New Infection
143
12764
5.0%
238
1.9%
11753
92.1%
985
7.7 %
711
5.6 %
62
0.5 %
Parallel - Other
7
812
0.3%
88
10.8%
676
83.3%
59
7.3 %
47
5.8 %
1
0.1 %
Parallel Persistent Infection
11
764
0.3%
78
10.2%
612
80.1%
101
13.2 %
74
9.7 %
0
Parallel - Rapid Testing Two-times IRs
40
157
0.1%
35
22.3%
114
72.6%
9
5.7 %
7
4.5 %
1
0.6 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
66
7153
2.8%
160
2.2%
6401
89.5%
547
7.6 %
378
5.3 %
214
3.0 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
1169
57607
22.7%
2869
5.0%
50144
87.0%
4721
8.2 %
3366
5.8 %
1228
2.1 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
0.9%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
0.9%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | January |
| February     | 206     |
| March        | 189     |
| April        | 195     |
| May          | 250     |
| June         | 187     |
| July         | 207     |
| August       | 228     |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| Totals (last |         |
| 12 months)   |         |
| 2669         | 253486  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1469
193638
1013
9620
710
0
0
0
0
780
16
12
33
1
7939
25
43
1
2
Scotland
31
2241
20
181
30
0
0
0
0
22
4
0
0
8
50
0
37
0
27
Wales
1169
57607
594
2869
229
0
0
0
0
140
7
2
18
3
1831
207
380
3
14
Totals
2669
253486
1627
12670
969
0
0
0
0
942
27
14
51
12
9820
232
460
4
43

*Includes all animals with IFNg test negative, resample and reject outcomes

County
%Resampled %Retested
| Berks         | 2.33 %   | 4.24 %   |
|---------------|----------|----------|
| Bucks         | 5.96 %   | 6.97 %   |
| Cambs         | 5.34 %   | 9.16 %   |
| Carms         | 4.60 %   | 7.41 %   |
| Ceredigion    | 5.15 %   | 6.19 %   |
| Cheshire      | 5.57 %   | 8.13 %   |
| Corn & Scilly | 6.29 %   | 8.31 %   |
| Cumbria       | 10.02 %  | 11.89 %  |
| Derbyshire    | 8.99 %   | 9.09 %   |
| Devon         | 5.28 %   | 7.26 %   |
| Dorset        | 4.07 %   | 6.47 %   |
| E Sussex      | 2.39 %   | 2.39 %   |
| Gloucs        | 3.30 %   | 4.51 %   |
| Gwent         | 8.33 %   | 10.19 %  |
| Gwynedd       | 2.54 %   | 6.76 %   |
| Hampshire     | 4.53 %   | 11.08 %  |
| Heref         | 8.05 %   | 9.17 %   |
| Isle of wight | 13.44 %  | 13.98 %  |
| Leics & Rut   | 2.91 %   | 3.74 %   |
| Lincs         |          |          |
| NE Wales      | 4.59 %   | 7.54 %   |
| Northants     | 14.63 %  | 14.63 %  |
| Oxon          | 8.62 %   | 12.27 %  |
| Pembs       | 4.84 %   | 6.89 %   |
|-------------|----------|----------|
| Powys       | 3.17 %   | 3.49 %   |
| Shropshire  | 4.32 %   | 5.76 %   |
| Somerset    | 1.84 %   | 1.84 %   |
| S Wales     | 3.37 %   | 4.49 %   |
| Warks       | 5.23 %   | 6.54 %   |
| W Glamorgan | 6.79 %   | 10.80 %  |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
15080
824
5.5%
15274
824
5.4%
Wales
6199
417
6.7%
6280
417
6.6%
GB
21279
1241
5.8%
21554
1241
5.8%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
7195
444
6.2%
7199
444
6.2%
Wales
1765
165
9.3%
1793
165
9.2%
Scotland
486
59
12.1%
486
59
12.1%
GB
9446
668
7.1%
9478
668
7.0%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
165901
9620
5.8%
193638
9620
5.0%
Wales
50414
2858
5.7%
57607
2869
5.0%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
218375
12659
5.8%
253486
12670
5.0%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
77307
5094
6.6%
98529
5094
5.2%
Wales
32133
2314
7.2%
35660
2314
6.5%
Scotland
2979
228
7.7%
3267
228
7.0%
GB
112416
7636
6.8%
137456
7636
5.6%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Northern Somerset & South Glou
PRV-SE
1
1
0
Oxfordshire
PRV-SE
1
2
1
50.00 %
Somerset excl North
PRV-SE
1
30
8
26.67 %
Total
3
33
9
27.27 %
Isle of Man
SER-IOM
2
2
0
* Test type
Num Submissions
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
3
3
1
33.33 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
PRV-SP
1
3
0
Northern Somerset & South Glou
PRV-SE
3
47
8
17.02 %
Oxfordshire
PRV-SE
2
40
5
12.50 %
Somerset excl North
PRV-SE
4
76
11
14.47 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
West Sussex
PRV-SP
1
1
0
Total
32
227
33
14.54 %
Isle of Man
SER-IOM
18
51
1
1.96 %

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

Private
% Positive
% Positive
Num Samples
Num Positives Private
|               | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|---------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077        | Carmarthenshire |        1 |                10 | 1                    | 10.0 %                            |
| Staffordshire | 1               |      382 |                91 | 23.8 %               |                                   |
| TC0077        | Sum:            |      392 |                92 | 23.5 %               |                                   |

## Table 8B Number Of Cattle Antibody Tests 2018

|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Argyll and Bute | 1        |                 3 | 3                    | 100.0 %                           |
| Carmarthenshire  | 4               | 192      |                 6 | 3.1 %                |                                   |
| Ceredigion       | 1               | 85       |                 4 | 4.7 %                |                                   |
| North East Wales | 2               | 66       |                 4 | 6.1 %                |                                   |
| Pembrokeshire    | 3               | 201      |                10 | 5.0 %                |                                   |
| Powys            | 2               | 361      |                 4 | 1.1 %                |                                   |
| South Wales      | 5               | 199      |                17 | 8.5 %                |                                   |
| Staffordshire    | 1               | 382      |                91 | 23.8 %               |                                   |
| TC0077           | Sum:            | 1,489    |               139 | 9.3 %                |                                   |