# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 147

# Report Period 1St - 30Th November 2018

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2018               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| November                |                          |          |
| 2018                    |                          |          |
| Submissions             | 282                      | 2441     |
| Samples                 | Total (%)                | 24570    |
| England                 | 18356                    | 74.71 %  |
| Wales                   | 6214                     | 25.29 %  |
| Scotland                | 2241                     | 0.97 %   |
| Parallel Tests          | PAR-CUL-N                | 4069     |
| PAR-CUL-P               | 131                      | 0.53 %   |
| PAR-HERD-S              | 805                      | 3.28 %   |
| PAR-NEW-IN              | 10843                    | 44.13 %  |
| PAR-OTHER               | 1421                     | 5.78 %   |
| PAR-PERSIS              | 6047                     | 24.61 %  |
| PAR-RAPID               | 192                      | 0.78 %   |
| Total(% of all samples) | 23508                    | 95.68 %  |
| Serial/Extended Tests   | SER-FLEXI                | 1062     |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 1062                     | 4.32 %   |
| Total                   | 24570                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2538     |
| England                 | 1992                     | 8.11 %   |
| Wales                   | 546                      | 2.22 %   |
| Scotland                | 201                      | 0.09 %   |
| Resamples               | Total (% of all samples) | 1626     |
| England                 | 1264                     | 5.14 %   |
| Wales                   | 362                      | 1.47 %   |
| Scotland                | 139                      | 0.06 %   |
| Rejects                 | Total (% of all samples) | 320      |
| England                 | 249                      | 1.01 %   |
| Wales                   | 71                       | 0.29 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   4069 | 16.6 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    131 | 0.5 %    |
| PAR-HERD-S  |    805 | 3.3 %    |
| PAR-NEW-IN  |  10843 | 44.1 %   |
| PAR-OTHER   |   1421 | 5.8 %    |
| PAR-PERSIS  |   6047 | 24.6 %   |
| PAR-RAPID   |    192 | 0.8 %    |
| SER-FLEXI   |   1062 | 4.3 %    |
| Total       |  24570 | 100.00 % |
Breakdown
of Samples
submitted
by County
| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Corn & Scilly  |
| Devon          | PAR-CUL-N      |
| PAR-OTHER      | 1              |
| PAR-PERSIS     | 1              |
| Dorset         | PAR-CUL-N      |
| PAR-PERSIS     | 5              |
| Gloucs         | PAR-CUL-N      |
| PAR-PERSIS     | 1              |
| Heref          | PAR-CUL-N      |
| Shropshire     | PAR-NEW-IN     |
| PAR-OTHER      | 1              |
| PAR-PERSIS     | 1              |
| Somerset       | PAR-CUL-P      |
| PAR-OTHER      | 1              |
| Staffs         | SER-FLEXI      |
| Wiltshire      | PAR-NEW-IN     |
| PAR-OTHER      | 3              |
| PAR-PERSIS     | 1              |
| SER-FLEXI      | 4              |
| England - Edge | Cheshire       |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| PAR-PERSIS     | 2              |
| Leics & Rut    | PAR-NEW-IN     |
| PAR-OTHER      | 2              |
| Notts          | PAR-NEW-IN     |
| Oxon           | PAR-NEW-IN     |
| PAR-PERSIS     | 6              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For November 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Warks                   | PAR-NEW-IN     |
| England - LRA           | Cumbria        |
| PAR-OTHER               | 3              |
| Isle of wight           | PAR-NEW-IN     |
| Lincs                   | PAR-NEW-IN     |
| England                 |                |
| 159                     | 18356          |
| Wales - High TB         | Carms          |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 10             |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 2              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 2              |
| Gwent                   | PAR-PERSIS     |
| PAR-RAPID               | 5              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 10             |
| PAR-RAPID               | 12             |
| SER-FLEXI               | 5              |
| Powys                   | PAR-HERD-S     |
| PAR-PERSIS              | 5              |
| PAR-RAPID               | 4              |
| S Wales                 | PAR-PERSIS     |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For November 2018 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| Ceredigion     | PAR-HERD-S     |
| PAR-RAPID      | 2              |
| SER-FLEXI      | 1              |
| NE Wales       | PAR-NEW-IN     |
| PAR-PERSIS     | 2              |
| PAR-RAPID      | 1              |
| SER-FLEXI      | 5              |
| Powys          | PAR-RAPID      |
| Shropshire     | PAR-NEW-IN     |
| W Glamorgan    | PAR-OTHER      |
| Wales - Low TB | Gwynedd        |
| NE Wales       | PAR-NEW-IN     |
| Wales          |                |
| 123            | 6214           |
| Grand Total    | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-PERSIS    | 8              |
| SER-FLEXI     | 2              |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 1              |
| PAR-OTHER     | 3              |
| PAR-PERSIS    | 22             |
| SER-FLEXI     | 9              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 18             |
| PAR-NEW-IN    | 9              |
| PAR-OTHER     | 6              |
| PAR-PERSIS    | 35             |
| SER-FLEXI     | 14             |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 18             |
| SER-FLEXI     | 1              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 9              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 5              |
| SER-FLEXI     | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| Heref          | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-NEW-IN     | 3              |
| PAR-PERSIS     | 4              |
| SER-FLEXI      | 2              |
| SER-FRAUD      | 2              |
| Shropshire     | PAR-NEW-IN     |
| PAR-OTHER      | 8              |
| PAR-PERSIS     | 13             |
| Somerset       | PAR-CUL-N      |
| PAR-CUL-P      | 4              |
| PAR-NEW-IN     | 1              |
| PAR-OTHER      | 6              |
| PAR-PERSIS     | 21             |
| Staffs         | PAR-PERSIS     |
| SER-FLEXI      | 4              |
| Wiltshire      | PAR-NEW-IN     |
| PAR-OTHER      | 5              |
| PAR-PERSIS     | 12             |
| SER-FLEXI      | 6              |
| SER-FRAUD      | 2              |
| W Midlands     | PAR-PERSIS     |
| SER-FRAUD      | 3              |
| England - Edge | Berks          |
| PAR-PERSIS     | 6              |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| PAR-PERSIS     | 10             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Derbyshire    | PAR-NEW-IN     |
| E Sussex      | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| Hampshire     | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 1              |
| SER-NSR       | 3              |
| Leics & Rut   | PAR-NEW-IN     |
| PAR-OTHER     | 2              |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| Oxon          | PAR-NEW-IN     |
| PAR-PERSIS    | 14             |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| England - LRA | Beds           |
| Cumbria       | PAR-NEW-IN     |
| PAR-OTHER     | 3              |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| Humberside    | PAR-NEW-IN     |
| Isle of wight | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |
| Lincs         | PAR-NEW-IN     |
| Merseyside    | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England         |                |
| 1347            | 178364         |
| Wales - High TB | Carms          |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 52             |
| PAR-RAPID       | 92             |
| SER-FLEXI       | 12             |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 17             |
| PAR-RAPID       | 32             |
| SER-FLEXI       | 10             |
| Gwent           | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 38             |
| PAR-RAPID       | 37             |
| SER-FLEXI       | 6              |
| Pembrokshire    | PAR-OTHER      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 5              |
| PAR-PERSIS      | 87             |
| PAR-RAPID       | 133            |
| SER-FLEXI       | 29             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FRAUD               | 2              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 22             |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 48             |
| PAR-RAPID               | 35             |
| SER-FLEXI               | 6              |
| S Wales                 | PAR-PERSIS     |
| PAR-RAPID               | 3              |
| W Glamorgan             | PAR-RAPID      |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 4              |
| Ceredigion              | PAR-HERD-S     |
| PAR-NEW-IN              | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 21             |
| SER-FLEXI               | 5              |
| Mid glamorgan           | PAR-PERSIS     |
| NE Wales                | PAR-HERD-S     |
| PAR-NEW-IN              | 124            |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 9              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 8              |
| PAR-RAPID               | 3              |
| Shropshire              | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| W Glamorgan         | PAR-OTHER      |
| SER-FLEXI           | 2              |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 3              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1063                | 51327          |
| Scotland - Scotland | Aberdeenshire  |
| Argyll & Bute       | PAR-NEW-IN     |
| SER-FLEXI           | 1              |
| Ayrshire            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| Perthshire          | PAR-NEW-IN     |
| West lothian        | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 31                  | 2241           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 3A. Summary Of Ifn Gamma Results By Country And Protocol November 2018

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 6            |
| Total           | 159          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 15           |
| Total           | 123          |
| GB              | Parallel     |
| Serial/Extended | 21           |
| Total           | 282          |
| 159.00          |              |
| 123.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2018)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 55           |
| Total           | 1347 178364  |
| Scotland        | Parallel     |
| Serial/Extended | 1            |
| Total           | 31           |
| Wales           | Parallel     |
| Serial/Extended | 83           |
| Total           | 1063         |
| GB              | Parallel     |
| Serial/Extended | 139          |
| Total           | 2441 231932  |

####### #######
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
21
1062
4.3%
127
12.0%
823
77.5%
161
15.2 %
110
10.4 %
2
0.2 %
Parallel - Herd or Group Slaughter
17
805
3.3%
67
8.3%
682
84.7%
76
9.4 %
53
6.6 %
3
0.4 %
Parallel New Infection
105
10843
44.1%
491
4.5%
9278
85.6%
1197
11.0 %
811
7.5 %
263
2.4 %
Parallel - new infection cull area
34
4069
16.6%
281
6.9%
3587
88.2%
345
8.5 %
182
4.5 %
19
0.5 %
Parallel - Other
16
1421
5.8%
102
7.2%
1215
85.5%
142
10.0 %
86
6.1 %
18
1.3 %
Parallel Persistent Infection
48
6047
24.6%
440
7.3%
5221
86.3%
571
9.4 %
371
6.1 %
15
0.2 %
Parallel - persistent infection cull area
3
131
0.5%
13
9.9%
113
86.3%
31
23.7 %
5
3.8 %
0
Parallel - Rapid Testing Two-times IRs
38
192
0.8%
59
30.7%
125
65.1%
15
7.8 %
8
4.2 %
0
Total
282
24570
100.0%
1580
6.4%
21044
85.6%
2538
10.3 %
1626
6.6 %
320
1.3 %
England - HRA
Extended Flexible IFN-GAMMA test
6
674
2.7%
76
11.3%
509
75.5%
131
19.4 %
88
13.1 %
1
0.1 %
Parallel New Infection
3
64
0.3%
1
1.6%
60
93.8%
3
4.7 %
3
4.7 %
0
Parallel - new infection cull area
34
4069
16.6%
281
6.9%
3587
88.2%
345
8.5 %
182
4.5 %
19
0.5 %
Parallel - Other
6
838
3.4%
45
5.4%
748
89.3%
72
8.6 %
28
3.3 %
17
2.0 %
Parallel Persistent Infection
9
2667
10.9%
161
6.0%
2367
88.8%
223
8.4 %
134
5.0 %
5
0.2 %
Parallel - persistent infection cull area
3
131
0.5%
13
9.9%
113
86.3%
31
23.7 %
5
3.8 %
0
England - HRA
61
8443
34.4%
577
6.8%
7384
87.5%
805
9.5 %
440
5.2 %
42
0.5 %
England - Edge
Parallel New Infection
76
6641
27.0%
310
4.7%
5739
86.4%
808
12.2 %
585
8.8 %
7
0.1 %
Parallel - Other
2
27
0.1%
1
3.7%
26
96.3%
1
3.7 %
0
0
Parallel Persistent Infection
8
569
2.3%
43
7.6%
495
87.0%
55
9.7 %
31
5.4 %
0
England - Edge
86
7237
29.5%
354
4.9%
6260
86.5%
864
11.9 %
616
8.5 %
7
0.1 %
England - LRA
Parallel New Infection
9
2400
9.8%
117
4.9%
1916
79.8%
273
11.4 %
167
7.0 %
200
8.3 %
Parallel - Other
3
276
1.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
England - LRA
12
2676
10.9%
147
5.5%
2121
79.3%
323
12.1 %
208
7.8 %
200
7.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
9
223
0.9%
37
16.6%
177
79.4%
15
6.7 %
8
3.6 %
1
0.4 %
Parallel - Herd or Group Slaughter
16
695
2.8%
47
6.8%
607
87.3%
59
8.5 %
39
5.6 %
2
0.3 %
Parallel - Other
2
92
0.4%
10
10.9%
77
83.7%
5
5.4 %
5
5.4 %
0
Parallel Persistent Infection
28
2523
10.3%
189
7.5%
2140
84.8%
268
10.6 %
184
7.3 %
10
0.4 %
Parallel - Rapid Testing Two-times IRs
32
162
0.7%
54
33.3%
104
64.2%
10
6.2 %
4
2.5 %
0
Wales - High TB
87
3695
15.0%
337
9.1%
3105
84.0%
357
9.7 %
240
6.5 %
13
0.4 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
6
165
0.7%
14
8.5%
137
83.0%
15
9.1 %
14
8.5 %
0
Parallel - Herd or Group Slaughter
1
110
0.4%
20
18.2%
75
68.2%
17
15.5 %
14
12.7 %
1
0.9 %
Parallel New Infection
10
646
2.6%
20
3.1%
599
92.7%
49
7.6 %
25
3.9 %
2
0.3 %
Parallel - Other
3
188
0.8%
16
8.5%
159
84.6%
14
7.4 %
12
6.4 %
1
0.5 %
Parallel Persistent Infection
3
288
1.2%
47
16.3%
219
76.0%
25
8.7 %
22
7.6 %
0
Parallel - Rapid Testing Two-times IRs
6
30
0.1%
5
16.7%
21
70.0%
5
16.7 %
4
13.3 %
0
Wales - Intermediate TB
29
1427
5.8%
122
8.5%
1210
84.8%
125
8.8 %
91
6.4 %
4
0.3 %
Wales - Low TB
Parallel New Infection
7
1092
4.4%
43
3.9%
964
88.3%
64
5.9 %
31
2.8 %
54
4.9 %
Wales - Low TB
7
1092
4.4%
43
3.9%
964
88.3%
64
5.9 %
31
2.8 %
54
4.9 %

## Table 4B.  Summary Of Ifn Gamma Results By Submission Reason  (Total 2018)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
125
8863
3.8%
687
7.8%
7584
85.6%
765
8.6 %
506
5.7 %
86
1.0 %
Parallel - Herd or Group Slaughter
106
6501
2.8%
291
4.5%
5393
83.0%
620
9.5 %
444
6.8 %
373
5.7 %
Parallel New Infection
1044 120352
51.9%
4609
3.8%
107924
89.7%
10342
8.6 %
7096
5.9 %
723
0.6 %
Parallel - new infection cull area
238
28707
12.4%
1610
5.6%
25362
88.3%
2385
8.3 %
1549
5.4 %
186
0.6 %
Parallel - Other
67
4006
1.7%
327
8.2%
3358
83.8%
289
7.2 %
194
4.8 %
127
3.2 %
Parallel Persistent Infection
436
55411
23.9%
3157
5.7%
48601
87.7%
4258
7.7 %
3036
5.5 %
617
1.1 %
Parallel - persistent infection cull area
35
6286
2.7%
352
5.6%
5541
88.1%
450
7.2 %
293
4.7 %
100
1.6 %
Parallel - Rapid Testing Two-times IRs
376
1507
0.6%
338
22.4%
1065
70.7%
98
6.5 %
73
4.8 %
31
2.1 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
2441 231932
100.0%
11429
4.9%
205061
88.4%
19220
8.3 %
13199
5.7 %
2243
1.0 %
England - HRA
Extended Flexible IFN-GAMMA test
43
6550
2.8%
517
7.9%
5699
87.0%
522
8.0 %
321
4.9 %
13
0.2 %
Parallel New Infection
22
2742
1.2%
158
5.8%
2406
87.7%
248
9.0 %
166
6.1 %
12
0.4 %
Parallel - new infection cull area
238
28707
12.4%
1610
5.6%
25362
88.3%
2385
8.3 %
1549
5.4 %
186
0.6 %
Parallel - Other
33
3153
1.4%
206
6.5%
2691
85.3%
210
6.7 %
130
4.1 %
126
4.0 %
Parallel Persistent Infection
142
29519
12.7%
1733
5.9%
26300
89.1%
2066
7.0 %
1425
4.8 %
61
0.2 %
Parallel - persistent infection cull area
35
6286
2.7%
352
5.6%
5541
88.1%
450
7.2 %
293
4.7 %
100
1.6 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
687
84291
36.3%
3474
4.1%
75591
89.7%
7328
8.7 %
4993
5.9 %
233
0.3 %
Parallel - Other
4
29
0.0%
1
3.4%
27
93.1%
2
6.9 %
1
3.4 %
0
Parallel Persistent Infection
35
4577
2.0%
253
5.5%
3903
85.3%
389
8.5 %
319
7.0 %
102
2.2 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
90
11408
4.9%
442
3.9%
10051
88.1%
1053
9.2 %
712
6.2 %
203
1.8 %
Parallel - Other
3
276
0.1%
30
10.9%
205
74.3%
50
18.1 %
41
14.9 %
0
Parallel Persistent Infection
3
627
0.3%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
1347 178364
76.9%
8796
4.9%
158545
88.9%
14748
8.3 %
9987
5.6 %
1036
0.6 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2018) Cont.

Wales - High TB
Extended Flexible IFN-GAMMA test
63
1888
0.8%
145
7.7%
1513
80.1%
211
11.2 %
157
8.3 %
73
3.9 %
Parallel - Herd or Group Slaughter
95
5482
2.4%
265
4.8%
4583
83.6%
383
7.0 %
263
4.8 %
371
6.8 %
Parallel New Infection
24
869
0.4%
21
2.4%
810
93.2%
52
6.0 %
36
4.1 %
2
0.2 %
Parallel - Other
21
301
0.1%
29
9.6%
262
87.0%
12
4.0 %
10
3.3 %
0
Parallel Persistent Infection
245
20027
8.6%
1079
5.4%
17302
86.4%
1680
8.4 %
1192
6.0 %
454
2.3 %
Parallel - Rapid Testing Two-times IRs
334
1337
0.6%
302
22.6%
939
70.2%
89
6.7 %
66
4.9 %
30
2.2 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
16
369
0.2%
24
6.5%
319
86.4%
29
7.9 %
26
7.0 %
0
Parallel - Herd or Group Slaughter
11
1019
0.4%
26
2.6%
810
79.5%
237
23.3 %
181
17.8 %
2
0.2 %
Parallel New Infection
136
12467
5.4%
230
1.8%
11480
92.1%
965
7.7 %
697
5.6 %
60
0.5 %
Parallel - Other
4
199
0.1%
21
10.6%
165
82.9%
14
7.0 %
12
6.0 %
1
0.5 %
Parallel Persistent Infection
10
656
0.3%
75
11.4%
513
78.2%
87
13.3 %
68
10.4 %
0
Parallel - Rapid Testing Two-times IRs
38
155
0.1%
34
21.9%
113
72.9%
9
5.8 %
7
4.5 %
1
0.6 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
56
6390
2.8%
143
2.2%
5680
88.9%
497
7.8 %
354
5.5 %
213
3.3 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
1063
51327
22.1%
2452
4.8%
44595
86.9%
4271
8.3 %
3073
6.0 %
1207
2.4 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
0.9%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
1.0%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2017         | December |
| 2018         | January  |
| February     | 206      |
| March        | 189      |
| April        | 195      |
| May          | 250      |
| June         | 187      |
| July         | 207      |
| August       | 228      |
| September    | 216      |
| October      | 274      |
| November     | 282      |
| Totals (last |          |
| 12 months)   |          |
| 2555         | 241410   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1393
185563
966
9240
491
0
0
0
0
769
16
11
26
0
7644
20
41
1
2
Scotland
33
2727
22
240
30
0
0
0
0
25
4
0
0
8
105
0
37
0
27
Wales
1129
53120
573
2617
250
0
0
0
0
109
8
2
18
2
1625
114
382
4
11
Totals
2555
241410
1561
12097
771
0
0
0
0
903
28
13
44
10
9374
134
460
5
40

*Includes all animals with IFNg test negative, resample and reject outcomes

County
%Resampled %Retested
| Carms         | 7.70 %   | 11.40 %   |
|---------------|----------|-----------|
| Ceredigion    | 9.09 %   | 10.96 %   |
| Cheshire      | 9.56 %   | 12.51 %   |
| Corn & Scilly | 4.63 %   | 8.26 %    |
| Cumbria       | 8.64 %   | 13.52 %   |
| Derbyshire    | 8.47 %   | 14.16 %   |
| Devon         | 3.27 %   | 9.80 %    |
| Dorset        | 4.21 %   | 6.92 %    |
| E Sussex      | 10.88 %  | 15.09 %   |
| Gloucs        | 13.48 %  | 18.09 %   |
| Gwent         | 9.38 %   | 9.38 %    |
| Gwynedd       | 2.32 %   | 5.70 %    |
| Heref         | 6.25 %   | 8.33 %    |
| Isle of wight | 3.42 %   | 5.38 %    |
| Leics & Rut   | 1.89 %   | 7.55 %    |
| Lincs         | 7.02 %   | 8.77 %    |
| NE Wales      | 4.31 %   | 6.82 %    |
| Notts         | 40.00 %  | 40.00 %   |
| Oxon          | 7.86 %   | 10.61 %   |
| Pembs         | 4.74 %   | 7.24 %    |
| Powys         | 9.32 %   | 11.18 %   |
| Shropshire    | 7.66 %   | 16.86 %   |
| Somerset      | 3.42 %   | 21.23 %   |
| Staffs      | 15.47 %   | 23.02 %   |
|-------------|-----------|-----------|
| S Wales     |           |           |
| Warks       | 6.33 %    | 8.65 %    |
| W Glamorgan | 5.71 %    | 5.71 %    |
| Wiltshire   | 3.37 %    | 7.49 %    |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
17996
1078
6.0%
18356
1078
5.9%
Wales
6081
502
8.3%
6214
502
8.1%
GB
24076
1580
6.6%
24570
1580
6.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
8519
666
7.8%
8696
666
7.7%
Wales
1592
183
11.5%
1615
183
11.3%
Scotland
342
112
32.7%
355
112
31.5%
GB
10453
961
9.2%
10666
961
9.0%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
154045
8796
5.7%
178364
8796
4.9%
Wales
44614
2443
5.5%
51327
2452
4.8%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
200719
11420
5.7%
231932
11429
4.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
72323
4650
6.4%
91330
4650
5.1%
Wales
30793
2149
7.0%
33867
2149
6.3%
Scotland
2707
169
6.2%
2781
169
6.1%
GB
105820
6968
6.6%
127978
6968
5.4%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Cheshire
PRV-SP
1
1
0
Hampshire
PRV-SP
1
3
0
West Sussex
PRV-SP
1
1
0
Total
3
5
0
* Test type
Num Submissions
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
3
3
1
33.33 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
PRV-SP
1
3
0
Northern Somerset & South Glou
PRV-SE
2
46
8
17.39 %
Oxfordshire
PRV-SE
1
38
4
10.53 %
Somerset excl North
PRV-SE
3
46
3
6.52 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
West Sussex
PRV-SP
1
1
0
Total
29
194
24
12.37 %
Isle of Man
SER-IOM
16
49
1
2.04 %

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

Private
% Positive
% Positive
Num Samples
Num Positives Private
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        1 |                12 | 1                    | 8.3 %                             |
| North East Wales | 2               |       66 |                 4 | 6.1 %                |                                   |
| Powys            | 1               |       16 |                 1 | 6.3 %                |                                   |
| TC0077           | Sum:            |       94 |                 6 | 6.4 %                |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Argyll and Bute | 1        |                 3 | 3                    | 100.0 %                           |
| Carmarthenshire  | 3               | 182      |                 5 | 2.7 %                |                                   |
| Ceredigion       | 1               | 85       |                 4 | 4.7 %                |                                   |
| North East Wales | 2               | 66       |                 4 | 6.1 %                |                                   |
| Pembrokeshire    | 3               | 201      |                10 | 5.0 %                |                                   |
| Powys            | 2               | 361      |                 4 | 1.1 %                |                                   |
| South Wales      | 5               | 199      |                17 | 8.5 %                |                                   |
| TC0077           | Sum:            | 1,097    |                47 | 4.3 %                |                                   |