# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 153

# Report Period 1St - 31St May 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| May 2019                |                          |          |
| Submissions             | 364                      | 1557     |
| Samples                 | Total (%)                | 28357    |
| England                 | 23479                    | 82.80 %  |
| Wales                   | 4385                     | 15.46 %  |
| Scotland                | 493                      | 1.74 %   |
| Parallel Tests          | PAR-CUL-N                | 8515     |
| PAR-CUL-P               | 833                      | 2.94 %   |
| PAR-HERD-S              | 246                      | 0.87 %   |
| PAR-NEW-IN              | 11509                    | 40.59 %  |
| PAR-OTHER               | 360                      | 1.27 %   |
| PAR-PERSIS              | 6362                     | 22.44 %  |
| PAR-RAPID               | 123                      | 0.43 %   |
| Total(% of all samples) | 27948                    | 98.56 %  |
| Serial/Extended Tests   | SER-FLEXI                | 409      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 409                      | 1.44 %   |
| Total                   | 28357                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1641     |
| England                 | 1360                     | 4.80 %   |
| Wales                   | 263                      | 0.93 %   |
| Scotland                | 18                       | 0.06 %   |
| Resamples               | Total (% of all samples) | 1217     |
| England                 | 1013                     | 3.57 %   |
| Wales                   | 190                      | 0.67 %   |
| Scotland                | 14                       | 0.05 %   |
| Rejects                 | Total (% of all samples) | 208      |
| England                 | 206                      | 0.73 %   |
| Wales                   | 2                        | 0.01 %   |
| Scotland                | 0                        | 0.00 %   |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   8515 | 30.0 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    833 | 2.9 %    |
| PAR-HERD-S  |    246 | 0.9 %    |
| PAR-NEW-IN  |  11509 | 40.6 %   |
| PAR-OTHER   |    360 | 1.3 %    |
| PAR-PERSIS  |   6362 | 22.4 %   |
| PAR-RAPID   |    123 | 0.4 %    |
| SER-FLEXI   |    409 | 1.4 %    |
| Total       |  28357 | 100.00 % |
Breakdown
of Samples
submitted
by County

## Table 2A.  Summary By County For May 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 4              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 1              |
| Dorset          | PAR-CUL-N      |
| SER-FLEXI       | 1              |
| Gloucs          | PAR-CUL-N      |
| PAR-HERD-S      | 3              |
| Heref           | PAR-CUL-N      |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 5              |
| Shropshire      | SER-FLEXI      |
| Somerset excl N | PAR-CUL-N      |
| PAR-PERSIS      | 3              |
| Staffs          | PAR-PERSIS     |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 1              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Cheshire        | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| England - LRA   | Cumbria        |
| Essex           | PAR-NEW-IN     |
| Herts           | PAR-NEW-IN     |
| I of Wight      | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| SER-FLEXI       | 1              |
| England         |                |
| 197             | 23479          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 5              |
| Ceredigion      | PAR-NEW-IN     |
| PAR-PERSIS      | 5              |
| PAR-RAPID       | 6              |
| SER-FLEXI       | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Gwent                   | PAR-OTHER      |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 1              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 22             |
| PAR-RAPID               | 8              |
| SER-FLEXI               | 6              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 5              |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 10             |
| PAR-RAPID               | 6              |
| SER-FLEXI               | 4              |
| S Wales                 | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-RAPID      |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 5              |
| PAR-RAPID               | 3              |
| Ceredigion              | PAR-RAPID      |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Powys               | PAR-NEW-IN     |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 1              |
| W Glamorgan         | PAR-RAPID      |
| Wales - Low TB      | Gwynedd        |
| NE Wales            | PAR-NEW-IN     |
| Wales               |                |
| 163                 | 4385           |
| Scotland - Scotland | Kirkcudbright  |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 4                   | 493            |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 4              |
| PAR-NEW-IN    | 1              |
| PAR-PERSIS    | 14             |
| SER-FLEXI     | 2              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 20             |
| PAR-NEW-IN    | 3              |
| PAR-OTHER     | 5              |
| PAR-PERSIS    | 16             |
| SER-FLEXI     | 5              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 6              |
| PAR-NEW-IN    | 1              |
| SER-FLEXI     | 2              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 3              |
| PAR-HERD-S    | 3              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 14             |
| Shropshire    | PAR-CUL-P      |
| PAR-NEW-IN    | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 5              |
| SER-FLEXI       | 4              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 6              |
| Staffs          | PAR-PERSIS     |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 2              |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 118            |
| PAR-PERSIS      | 4              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| SER-NSR         | 2              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-PERSIS      | 3              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Essex           | PAR-NEW-IN     |
| Gr Manchester   | PAR-NEW-IN     |
| Herts           | PAR-NEW-IN     |
| I of Wight      | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| SER-FLEXI       | 2              |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 915             | 114874         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 6              |
| PAR-PERSIS      | 42             |
| PAR-RAPID       | 39             |
| SER-FLEXI       | 22             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 14             |
| PAR-RAPID       | 21             |
| SER-FLEXI       | 3              |
| Gwent           | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 9              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 19             |
| SER-FLEXI               | 1              |
| Pembrokeshire           | PAR-RAPID      |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 12             |
| PAR-PERSIS              | 62             |
| PAR-RAPID               | 55             |
| SER-FLEXI               | 15             |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 12             |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 23             |
| PAR-RAPID               | 32             |
| SER-FLEXI               | 7              |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 6              |
| PAR-RAPID               | 5              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 11             |
| SER-FLEXI           | 22             |
| Powys               | PAR-NEW-IN     |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 1              |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 2              |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| Wales               |                |
| 635                 | 28125          |
| Scotland - Scotland | Ayrshire       |
| Kirkcudbright       | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 7                   | 859            |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 3A. Summary Of Ifn Gamma Results By Country And Protocol May 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 4            |
| Total           | 197          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 4            |
| Wales           | Parallel     |
| Serial/Extended | 22           |
| Total           | 163          |
| GB              | Parallel     |
| Serial/Extended | 26           |
| Total           | 364          |
| 197.00          |              |
| 163.00          |              |
| 4.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 19           |
| Total           | 915 114874   |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 7            |
| Wales           | Parallel     |
| Serial/Extended | 74           |
| Total           | 635          |
| GB              | Parallel     |
| Serial/Extended | 93           |
| Total           | 1557 143858  |
| 915.00          |              |
| 635.00          |              |
| 7.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
26
409
1.4%
44
10.8%
346
84.6%
20
4.9 %
19
4.6 %
0
Parallel - Herd or Group Slaughter
16
246
0.9%
19
7.7%
213
86.6%
18
7.3 %
14
5.7 %
0
Parallel New Infection
117
11509
40.6%
316
2.7%
10635
92.4%
709
6.2 %
558
4.8 %
0
Parallel - new infection cull area
72
8515
30.0%
427
5.0%
7732
90.8%
495
5.8 %
356
4.2 %
0
Parallel - Other
10
360
1.3%
25
6.9%
328
91.1%
14
3.9 %
7
1.9 %
0
Parallel Persistent Infection
70
6362
22.4%
242
3.8%
5681
89.3%
340
5.3 %
231
3.6 %
208
3.3 %
Parallel - persistent infection cull area
5
833
2.9%
24
2.9%
779
93.5%
40
4.8 %
30
3.6 %
0
Parallel - Rapid Testing Two-times IRs
48
123
0.4%
19
15.4%
102
82.9%
5
4.1 %
2
1.6 %
0
Total
364
28357
100.0%
1116
3.9%
25816
91.0%
1641
5.8 %
1217
4.3 %
208
0.7 %
England - HRA
Extended Flexible IFN-GAMMA test
3
30
0.1%
2
6.7%
23
76.7%
6
20.0 %
5
16.7 %
0
Parallel - Herd or Group Slaughter
3
32
0.1%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
Parallel New Infection
2
195
0.7%
6
3.1%
174
89.2%
16
8.2 %
15
7.7 %
0
Parallel - new infection cull area
72
8515
30.0%
427
5.0%
7732
90.8%
495
5.8 %
356
4.2 %
0
Parallel - Other
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Parallel Persistent Infection
17
3425
12.1%
153
4.5%
2963
86.5%
157
4.6 %
103
3.0 %
206
6.0 %
Parallel - persistent infection cull area
5
833
2.9%
24
2.9%
779
93.5%
40
4.8 %
30
3.6 %
0
England - HRA
104
13032
46.0%
612
4.7%
11703
89.8%
717
5.5 %
511
3.9 %
206
1.6 %
England - Edge
Parallel New Infection
78
9394
33.1%
231
2.5%
8745
93.1%
543
5.8 %
418
4.4 %
0
Parallel Persistent Infection
2
175
0.6%
3
1.7%
166
94.9%
8
4.6 %
6
3.4 %
0
England - Edge
80
9569
33.7%
234
2.4%
8911
93.1%
551
5.8 %
424
4.4 %
0
England - LRA
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Parallel New Infection
12
877
3.1%
18
2.1%
781
89.1%
92
10.5 %
78
8.9 %
0
England - LRA
13
878
3.1%
18
2.1%
782
89.1%
92
10.5 %
78
8.9 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
18
298
1.1%
33
11.1%
252
84.6%
13
4.4 %
13
4.4 %
0
Parallel - Herd or Group Slaughter
13
214
0.8%
19
8.9%
183
85.5%
15
7.0 %
12
5.6 %
0
Parallel New Infection
8
181
0.6%
4
2.2%
169
93.4%
8
4.4 %
8
4.4 %
0
Parallel - Other
6
48
0.2%
15
31.3%
33
68.8%
4
8.3 %
0
0
Parallel Persistent Infection
45
2088
7.4%
61
2.9%
1923
92.1%
137
6.6 %
102
4.9 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
34
88
0.3%
15
17.0%
72
81.8%
4
4.5 %
1
1.1 %
0
Wales - High TB
124
2917
10.3%
147
5.0%
2632
90.2%
181
6.2 %
136
4.7 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
80
0.3%
9
11.3%
70
87.5%
1
1.3 %
1
1.3 %
0
Parallel New Infection
10
147
0.5%
1
0.7%
129
87.8%
19
12.9 %
17
11.6 %
0
Parallel - Other
2
310
1.1%
10
3.2%
293
94.5%
10
3.2 %
7
2.3 %
0
Parallel Persistent Infection
6
674
2.4%
25
3.7%
629
93.3%
38
5.6 %
20
3.0 %
0
Parallel - Rapid Testing Two-times IRs
13
34
0.1%
4
11.8%
29
85.3%
1
2.9 %
1
2.9 %
0
Wales - Intermediate TB
35
1245
4.4%
49
3.9%
1150
92.4%
69
5.5 %
46
3.7 %
0
Wales - Low TB
Parallel New Infection
3
222
0.8%
2
0.9%
212
95.5%
13
5.9 %
8
3.6 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
4
223
0.8%
2
0.9%
213
95.5%
13
5.8 %
8
3.6 %
0
Scotland - Scotland
Parallel New Infection
4
493
1.7%
54
11.0%
425
86.2%
18
3.7 %
14
2.8 %
0
Scotland - Scotland
4
493
1.7%
54
11.0%
425
86.2%
18
3.7 %
14
2.8 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
90
3115
2.2%
230
7.4%
2645
84.9%
181
5.8 %
140
4.5 %
100
3.2 %
Parallel - Herd or Group Slaughter
88
3187
2.2%
231
7.2%
2720
85.3%
278
8.7 %
232
7.3 %
5
0.2 %
Parallel New Infection
609
69859
48.6%
2710
3.9%
62302
89.2%
4935
7.1 %
4058
5.8 %
789
1.1 %
Parallel - new infection cull area
243
29207
20.3%
1588
5.4%
25742
88.1%
1906
6.5 %
1514
5.2 %
363
1.2 %
Parallel - Other
46
2820
2.0%
218
7.7%
2399
85.1%
202
7.2 %
161
5.7 %
42
1.5 %
Parallel Persistent Infection
241
26832
18.7%
1399
5.2%
23581
87.9%
1777
6.6 %
1407
5.2 %
448
1.7 %
Parallel - persistent infection cull area
40
8208
5.7%
469
5.7%
7274
88.6%
551
6.7 %
458
5.6 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
197
613
0.4%
142
23.2%
412
67.2%
46
7.5 %
33
5.4 %
26
4.2 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
1557 143858
100.0%
6987
4.9%
127092
88.3%
9876
6.9 %
8003
5.6 %
1780
1.2 %
England - HRA
Extended Flexible IFN-GAMMA test
14
998
0.7%
62
6.2%
847
84.9%
69
6.9 %
44
4.4 %
45
4.5 %
Parallel - Herd or Group Slaughter
3
32
0.0%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
Parallel New Infection
8
1009
0.7%
114
11.3%
791
78.4%
125
12.4 %
104
10.3 %
0
Parallel - new infection cull area
242
28947
20.1%
1569
5.4%
25513
88.1%
1894
6.5 %
1502
5.2 %
363
1.3 %
Parallel - Other
14
836
0.6%
77
9.2%
665
79.5%
61
7.3 %
52
6.2 %
42
5.0 %
Parallel Persistent Infection
69
10590
7.4%
698
6.6%
8920
84.2%
699
6.6 %
546
5.2 %
427
4.0 %
Parallel - persistent infection cull area
40
8208
5.7%
469
5.7%
7274
88.6%
551
6.7 %
458
5.6 %
7
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
462
58011
40.3%
2187
3.8%
51883
89.4%
3979
6.9 %
3258
5.6 %
683
1.2 %
Parallel - new infection cull area
1
260
0.2%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
10
1012
0.7%
20
2.0%
957
94.6%
41
4.1 %
35
3.5 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel New Infection
47
4904
3.4%
167
3.4%
4334
88.4%
429
8.7 %
365
7.4 %
38
0.8 %
England
915 114874
79.9%
5383
4.7%
101508
88.4%
7866
6.8 %
6379
5.6 %
1605
1.4 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Wales - High TB
Extended Flexible IFN-GAMMA test
51
1425
1.0%
104
7.3%
1253
87.9%
74
5.2 %
66
4.6 %
2
0.1 %
Parallel - Herd or Group Slaughter
81
3129
2.2%
228
7.3%
2677
85.6%
265
8.5 %
220
7.0 %
5
0.2 %
Parallel New Infection
17
644
0.4%
43
6.7%
568
88.2%
35
5.4 %
33
5.1 %
0
Parallel - Other
22
1434
1.0%
90
6.3%
1263
88.1%
107
7.5 %
81
5.6 %
0
Parallel Persistent Infection
154
14435
10.0%
644
4.5%
12969
89.8%
996
6.9 %
803
5.6 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
171
535
0.4%
129
24.1%
349
65.2%
44
8.2 %
31
5.8 %
26
4.9 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
23
642
0.4%
63
9.8%
497
77.4%
35
5.5 %
29
4.5 %
53
8.3 %
Parallel - Herd or Group Slaughter
4
26
0.0%
3
11.5%
13
50.0%
10
38.5 %
10
38.5 %
0
Parallel New Infection
53
3998
2.8%
131
3.3%
3561
89.1%
308
7.7 %
254
6.4 %
52
1.3 %
Parallel - Other
10
550
0.4%
51
9.3%
471
85.6%
34
6.2 %
28
5.1 %
0
Parallel Persistent Infection
8
795
0.6%
37
4.7%
735
92.5%
41
5.2 %
23
2.9 %
0
Parallel - Rapid Testing Two-times IRs
25
77
0.1%
13
16.9%
62
80.5%
2
2.6 %
2
2.6 %
0
Wales - Low TB
Parallel New Infection
15
434
0.3%
10
2.3%
395
91.0%
21
4.8 %
13
3.0 %
16
3.7 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
635
28125
19.6%
1546
5.5%
24814
88.2%
1972
7.0 %
1593
5.7 %
175
0.6 %
Scotland - Scotland
Parallel New Infection
7
859
0.6%
58
6.8%
770
89.6%
38
4.4 %
31
3.6 %
0
Scotland
7
859
0.6%
58
6.8%
770
89.6%
38
4.4 %
31
3.6 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | June    |
| July         | 207     |
| August       | 228     |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| April        | 269     |
| May          | 364     |
| Totals (last |         |
| 12 months)   |         |
| 3179         | 298000  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1831
234237
1229
10796
112
0
0
0
0
863
16
4
36
3
9611
31
79
0
2
Scotland
16
1309
8
80
8
0
0
0
0
6
0
3
0
0
60
0
3
0
0
Wales
1332
62454
672
3484
39
0
0
0
0
170
9
3
25
2
2546
59
579
3
29
Totals
3179
298000
1909
14360
159
0
0
0
0
1039
25
10
61
5
12217
90
661
3
31

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Avon          | 17.65 %  | 17.65 %                |
| Berks         | 4.36 %   | 5.60 %                 |
| Bucks         | 13.16 %  | 13.16 %                |
| Carms         | 3.02 %   | 5.72 %                 |
| Ceredigion    | 5.86 %   | 6.28 %                 |
| Cheshire      | 4.04 %   | 5.18 %                 |
| Corn & Scilly | 2.68 %   | 3.42 %                 |
| Cumbria       | 7.89 %   | 9.81 %                 |
| Derbyshire    | 4.10 %   | 5.62 %                 |
| Devon         | 3.62 %   | 5.08 %                 |
| Dorset        | 5.69 %   | 7.51 %                 |
| Essex         |          |                        |
| E Sussex      | 5.33 %   | 6.56 %                 |
| Gloucs        | 6.06 %   | 7.58 %                 |
| Gwent         | 1.99 %   | 2.65 %                 |
| Gwynedd       |          |                        |
| Hampshire     | 17.24 %  | 20.69 %                |
| Heref         | 3.66 %   | 5.36 %                 |
| Herts         | 22.64 %  | 24.53 %                |
| I of Wight    | 8.07 %   | 8.07 %                 |
| Kirkcudbright | 3.06 %   | 3.57 %                 |
| Leics & Rut   | 5.14 %   | 5.53 %                 |
| NE Wales      | 4.62 %   | 6.69 %                 |
| Northants       | 3.47 %   | 6.73 %   |
|-----------------|----------|----------|
| Notts           | 2.74 %   | 4.11 %   |
| N Yorks         | 8.29 %   | 10.36 %  |
| Oxon            | 5.59 %   | 6.95 %   |
| Pembs           | 5.71 %   | 7.26 %   |
| Powys           | 6.07 %   | 6.54 %   |
| S Glamorgan     |          |          |
| Shropshire      | 20.00 %  | 20.00 %  |
| Somerset excl N | 4.68 %   | 6.35 %   |
| Staffs          | 4.19 %   | 8.14 %   |
| S Wales         | 0.82 %   | 0.82 %   |
| Warks           | 5.26 %   | 6.31 %   |
W Glamorgan Wigtown
1.98 %
3.96 %
Wiltshire
4.87 %
6.48 %
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23289
864
3.7%
23479
864
3.7%
Wales
4302
198
4.6%
4385
198
4.5%
Scotland
493
54
11.0%
493
54
11.0%
GB
28083
1116
4.0%
28357
1116
3.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
16310
837
5.1%
16507
837
5.1%
Wales
3910
85
2.2%
4021
85
2.1%
Scotland
522
25
4.8%
538
25
4.6%
GB
20742
947
4.6%
21066
947
4.5%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
108872
5383
4.9%
114874
5383
4.7%
Wales
26100
1546
5.9%
28125
1546
5.5%
Scotland
859
58
6.8%
859
58
6.8%
GB
135830
6987
5.1%
143858
6987
4.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
69959
4207
6.0%
74275
4207
5.7%
Wales
21403
931
4.3%
23278
931
4.0%
Scotland
1689
159
9.4%
1791
159
8.9%
GB
93040
5297
5.7%
99344
5297
5.3%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SE
1
6
0
0
Herefordshire
PRV-SP
1
2
0
0
Total
2
8
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Devon               | PRV-SE        |
| Gloucestershire     | PRV-SP        |
| Herefordshire       | PRV-SP        |
| Somerset excl North | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 9                   |               |
| 61                  |               |
| 15                  | 24.59 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|               | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|---------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077        | Carmarthenshire |        2 |                74 | 11                   | 14.9%                             |
| Ceredigion    | 1               |       29 |                 7 | 24.1%                |                                   |
| Pembrokeshire | 3               |      102 |                 4 | 3.9%                 |                                   |
| Powys         | 1               |        6 |                 1 | 16.7%                |                                   |
| South Wales   | 1               |       37 |                 1 | 2.7%                 |                                   |
| TC0077        | Sum:            |      248 |                24 | 9.7 %                |                                   |
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        4 |               199 | 15                   | 7.5%                              |
| Ceredigion       | 1               |       29 |                 7 | 24.1%                |                                   |
| North East Wales | 11              |      383 |                63 | 16.4%                |                                   |
| Pembrokeshire    | 5               |      166 |                 5 | 3.0%                 |                                   |
| Powys            | 1               |        6 |                 1 | 16.7%                |                                   |
| South Wales      | 3               |      155 |                14 | 9.0%                 |                                   |
| TC0077           | Sum:            |      938 |               105 | 11.2 %               |                                   |