# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 158

# Report Period 1St - 31St October 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| October                 |                          |          |
| 2019                    |                          |          |
| Submissions             | 298                      | 2965     |
| Samples                 | Total (%)                | 26690    |
| England                 | 20315                    | 76.11 %  |
| Wales                   | 5904                     | 22.12 %  |
| Scotland                | 471                      | 1.76 %   |
| Parallel Tests          | PAR-CUL-N                | 5925     |
| PAR-CUL-P               | 1760                     | 6.59 %   |
| PAR-HERD-S              | 2536                     | 9.50 %   |
| PAR-NEW-IN              | 12721                    | 47.66 %  |
| PAR-OTHER               | 61                       | 0.23 %   |
| PAR-PERSIS              | 3206                     | 12.01 %  |
| PAR-RAPID               | 301                      | 1.13 %   |
| Total(% of all samples) | 26510                    | 99.33 %  |
| Serial/Extended Tests   | SER-FLEXI                | 177      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 3                        | 0.01 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 180                      | 0.67 %   |
| Total                   | 26690                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1641     |
| England                 | 1296                     | 4.86 %   |
| Wales                   | 321                      | 1.20 %   |
| Scotland                | 24                       | 0.09 %   |
| Resamples               | Total (% of all samples) | 1293     |
| England                 | 1046                     | 3.92 %   |
| Wales                   | 228                      | 0.85 %   |
| Scotland                | 19                       | 0.07 %   |
| Rejects                 | Total (% of all samples) | 750      |
| England                 | 715                      | 2.68 %   |
| Wales                   | 25                       | 0.09 %   |
| Scotland                | 10                       | 0.04 %   |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   5925 | 22.2 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   1760 | 6.6 %    |
| PAR-HERD-S  |   2536 | 9.5 %    |
| PAR-NEW-IN  |  12721 | 47.7 %   |
| PAR-OTHER   |     61 | 0.2 %    |
| PAR-PERSIS  |   3206 | 12.0 %   |
| PAR-RAPID   |    301 | 1.1 %    |
| SER-FLEXI   |    177 | 0.7 %    |
| SER-NSR     |      3 | 0.0 %    |
| Total       |  26690 | 100.00 % |
Breakdown
of Samples
submitted
by County

Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-PERSIS      | 2              |
| SER-FLEXI       | 2              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 2              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-P      |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 1              |
| Shropshire      | PAR-OTHER      |
| Somerset excl N | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| PAR-PERSIS      | 1              |
| England - Edge  | Bucks          |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-PERSIS     |
| SER-NSR         | 1              |
| Northants       | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-PERSIS      | 2              |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| Northumb        | PAR-NEW-IN     |
| Suffolk         | PAR-NEW-IN     |
| England         |                |
| 169             | 20315          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 8              |
| PAR-RAPID       | 6              |
| SER-FLEXI       | 5              |
| Ceredigion      | PAR-PERSIS     |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-OTHER      |
| PAR-PERSIS      | 2              |
| PAR-RAPID       | 2              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 15             |
| SER-FLEXI       | 1              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 5              |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FLEXI               | 3              |
| Wales - Intermediate TB | Carms          |
| Ceredigion              | PAR-RAPID      |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 3              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | PAR-RAPID      |
| Shropshire              | PAR-NEW-IN     |
| W Glamorgan             | PAR-PERSIS     |
| Wales - Low TB          | Gwynedd        |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 1              |
| Wales                   |                |
| 124                     | 5904           |
| Scotland - Scotland     | Dumfries & G   |
| Kirkcudbright           | PAR-CUL-P      |
| PAR-NEW-IN              | 1              |
| Scotland                |                |
| 5                       | 471            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-OTHER     | 4              |
| PAR-PERSIS    | 9              |
| SER-FRAUD     | 2              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 4              |
| PAR-NEW-IN    | 7              |
| PAR-PERSIS    | 33             |
| SER-FLEXI     | 4              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 28             |
| PAR-NEW-IN    | 4              |
| PAR-OTHER     | 7              |
| PAR-PERSIS    | 22             |
| SER-FLEXI     | 8              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 17             |
| PAR-NEW-IN    | 5              |
| SER-FLEXI     | 4              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 10             |
| PAR-HERD-S    | 4              |
| PAR-NEW-IN    | 1              |
| SER-FLEXI     | 2              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 4              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 30             |
| Shropshire      | PAR-CUL-P      |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 10             |
| SER-FLEXI       | 5              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 13             |
| Staffs          | PAR-OTHER      |
| PAR-PERSIS      | 7              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 24             |
| PAR-NEW-IN      | 7              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 10             |
| SER-FLEXI       | 1              |
| Worcs           | PAR-PERSIS     |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 209            |
| PAR-PERSIS      | 5              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Hampshire     | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 2              |
| Leics & Rut   | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| Oxon          | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 6              |
| England - LRA | Cambs          |
| Cumbria       | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Gr London     | PAR-NEW-IN     |
| Gr Manchester | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| I of Wight    | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |
| Lancashire    | PAR-NEW-IN     |
| Lincs         | PAR-NEW-IN     |
| Northumb      | PAR-NEW-IN     |
| PAR-PERSIS    | 3              |
| N Yorks       | PAR-NEW-IN     |
| PAR-PERSIS    | 3              |
| SER-FLEXI     | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Suffolk         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| W Yorks         | PAR-HERD-S     |
| England         |                |
| 1747            | 221109         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 16             |
| PAR-PERSIS      | 72             |
| PAR-RAPID       | 68             |
| SER-FLEXI       | 42             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 31             |
| PAR-RAPID       | 49             |
| SER-FLEXI       | 9              |
| Gwent           | PAR-HERD-S     |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 18             |
| PAR-RAPID       | 26             |
| SER-FLEXI       | 1              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 17             |
| PAR-PERSIS      | 122            |
| PAR-RAPID       | 117            |
| SER-FLEXI       | 42             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 29             |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 38             |
| PAR-RAPID               | 59             |
| SER-FLEXI               | 14             |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 11             |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 10             |
| PAR-PERSIS              | 10             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-RAPID           | 15             |
| SER-FLEXI           | 41             |
| Powys               | PAR-NEW-IN     |
| PAR-RAPID           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 3              |
| Shropshire          | PAR-NEW-IN     |
| S Wales             | PAR-RAPID      |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 2              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 1197                | 52791          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-CUL-P      |
| PAR-NEW-IN          | 5              |
| PAR-PERSIS          | 1              |
| Wigtown             | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| Scotland    |                |
| 21          | 2688           |
| Grand Total | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol October 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 3            |
| Total           | 169          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 5            |
| Wales           | Parallel     |
| Serial/Extended | 13           |
| Total           | 124          |
| GB              | Parallel     |
| Serial/Extended | 16           |
| Total           | 298          |
| 169.00          |              |
| 124.00          |              |
| 5.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 31           |
| Total           | 1747 221109  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 21           |
| Wales           | Parallel     |
| Serial/Extended | 160          |
| Total           | 1197         |
| GB              | Parallel     |
| Serial/Extended | 191          |
| Total           | 2965 276588  |

####### #######
21.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
177
0.7%
9
5.1%
162
91.5%
9
5.1 %
6
3.4 %
0
Parallel - Herd or Group Slaughter
14
2536
9.5%
68
2.7%
2397
94.5%
106
4.2 %
68
2.7 %
3
0.1 %
Parallel New Infection
111
12721
47.7%
236
1.9%
11337
89.1%
765
6.0 %
633
5.0 %
519
4.1 %
Parallel - new infection cull area
53
5925
22.2%
149
2.5%
5239
88.4%
447
7.5 %
344
5.8 %
193
3.3 %
Parallel - Other
10
61
0.2%
15
24.6%
38
62.3%
9
14.8 %
8
13.1 %
0
Parallel Persistent Infection
37
3206
12.0%
157
4.9%
2879
89.8%
197
6.1 %
147
4.6 %
23
0.7 %
Parallel - persistent infection cull area
13
1760
6.6%
79
4.5%
1609
91.4%
91
5.2 %
72
4.1 %
0
Parallel - Rapid Testing Two-times IRs
44
301
1.1%
30
10.0%
244
81.1%
17
5.6 %
15
5.0 %
12
4.0 %
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Total
298
26690
100.0%
743
2.8%
23908
89.6%
1641
6.1 %
1293
4.8 %
750
2.8 %
England - HRA
Extended Flexible IFN-GAMMA test
2
28
0.1%
0
0.0 %
26
92.9%
2
7.1 %
2
7.1 %
0
Parallel New Infection
2
14
0.1%
0
0.0 %
12
85.7%
2
14.3 %
2
14.3 %
0
Parallel - new infection cull area
53
5925
22.2%
149
2.5%
5239
88.4%
447
7.5 %
344
5.8 %
193
3.3 %
Parallel - Other
2
6
0.0%
0
0.0 %
3
50.0%
3
50.0 %
3
50.0 %
0
Parallel Persistent Infection
7
1055
4.0%
43
4.1%
945
89.6%
61
5.8 %
54
5.1 %
13
1.2 %
Parallel - persistent infection cull area
12
1408
5.3%
72
5.1%
1269
90.1%
83
5.9 %
67
4.8 %
0
England - HRA
78
8436
31.6%
264
3.1%
7494
88.8%
598
7.1 %
472
5.6 %
206
2.4 %
England - Edge
Parallel New Infection
78
10272
38.5%
187
1.8%
9137
89.0%
538
5.2 %
442
4.3 %
506
4.9 %
Parallel Persistent Infection
3
675
2.5%
25
3.7%
617
91.4%
39
5.8 %
30
4.4 %
3
0.4 %
Serial - Suspected NSR Herd
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
England - Edge
82
10950
41.0%
212
1.9%
9757
89.1%
577
5.3 %
472
4.3 %
509
4.6 %
England - LRA
Parallel New Infection
8
794
3.0%
3
0.4%
699
88.0%
102
12.8 %
92
11.6 %
0
Parallel Persistent Infection
1
135
0.5%
4
3.0%
121
89.6%
19
14.1 %
10
7.4 %
0
England - LRA
9
929
3.5%
7
0.8%
820
88.3%
121
13.0 %
102
11.0 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
10
120
0.4%
9
7.5%
108
90.0%
6
5.0 %
3
2.5 %
0
Parallel - Herd or Group Slaughter
14
2536
9.5%
68
2.7%
2397
94.5%
106
4.2 %
68
2.7 %
3
0.1 %
Parallel New Infection
5
398
1.5%
25
6.3%
363
91.2%
12
3.0 %
10
2.5 %
0
Parallel - Other
5
31
0.1%
15
48.4%
15
48.4%
1
3.2 %
1
3.2 %
0
Parallel Persistent Infection
23
981
3.7%
64
6.5%
880
89.7%
44
4.5 %
30
3.1 %
7
0.7 %
Parallel - Rapid Testing Two-times IRs
37
149
0.6%
24
16.1%
103
69.1%
12
8.1 %
10
6.7 %
12
8.1 %
Wales - High TB
94
4215
15.8%
205
4.9%
3866
91.7%
181
4.3 %
122
2.9 %
22
0.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
3
29
0.1%
0
0.0 %
28
96.6%
1
3.4 %
1
3.4 %
0
Parallel New Infection
9
729
2.7%
16
2.2%
667
91.5%
55
7.5 %
43
5.9 %
3
0.4 %
Parallel - Other
2
7
0.0%
0
0.0 %
3
42.9%
4
57.1 %
4
57.1 %
0
Parallel Persistent Infection
3
360
1.3%
21
5.8%
316
87.8%
34
9.4 %
23
6.4 %
0
Parallel - Rapid Testing Two-times IRs
6
151
0.6%
6
4.0%
140
92.7%
5
3.3 %
5
3.3 %
0
Wales - Intermediate TB
23
1276
4.8%
43
3.4%
1154
90.4%
99
7.8 %
76
6.0 %
3
0.2 %
Wales - Low TB
Parallel New Infection
5
395
1.5%
5
1.3%
364
92.2%
40
10.1 %
30
7.6 %
0
Parallel - Other
1
17
0.1%
0
0.0 %
17
100.0%
1
5.9 %
0
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
7
413
1.5%
5
1.2%
382
92.5%
41
9.9 %
30
7.3 %
0
Scotland - Scotland
Parallel New Infection
4
119
0.4%
0
0.0 %
95
79.8%
16
13.4 %
14
11.8 %
10
8.4 %
Parallel - persistent infection cull area
1
352
1.3%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland - Scotland
5
471
1.8%
7
1.5%
435
92.4%
24
5.1 %
19
4.0 %
10
2.1 %
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
186
5820
2.1%
385
6.6%
5119
88.0%
297
5.1 %
215
3.7 %
101
1.7 %
Parallel - Herd or Group Slaughter
144
7342
2.7%
434
5.9%
6546
89.2%
475
6.5 %
352
4.8 %
11
0.1 %
Parallel New Infection
1090 126794
45.8%
4041
3.2%
114140
90.0%
9293
7.3 %
7322
5.8 %
1313
1.0 %
Parallel - new infection cull area
517
60714
22.0%
2768
4.6%
53966
88.9%
4430
7.3 %
3421
5.6 %
559
0.9 %
Parallel - Other
89
4174
1.5%
278
6.7%
3459
82.9%
485
11.6 %
395
9.5 %
42
1.0 %
Parallel Persistent Infection
470
52750
19.1%
2295
4.4%
47273
89.6%
3291
6.2 %
2528
4.8 %
666
1.3 %
Parallel - persistent infection cull area
92
17715
6.4%
907
5.1%
15869
89.6%
1204
6.8 %
932
5.3 %
7
0.0 %
Parallel - Rapid Testing Two-times IRs
372
1250
0.5%
222
17.8%
931
74.5%
78
6.2 %
58
4.6 %
39
3.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Total
2965 276588
100.0%
11330
4.1%
247331
89.4%
19554
7.1 %
15224
5.5 %
2738
1.0 %
England - HRA
Extended Flexible IFN-GAMMA test
24
1634
0.6%
79
4.8%
1448
88.6%
97
5.9 %
62
3.8 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
26
2102
0.8%
139
6.6%
1755
83.5%
264
12.6 %
208
9.9 %
0
Parallel - new infection cull area
515
60416
21.8%
2747
4.5%
53708
88.9%
4409
7.3 %
3402
5.6 %
559
0.9 %
Parallel - Other
26
1667
0.6%
88
5.3%
1283
77.0%
301
18.1 %
254
15.2 %
42
2.5 %
Parallel Persistent Infection
135
22098
8.0%
1111
5.0%
19349
87.6%
1450
6.6 %
1098
5.0 %
541
2.4 %
Parallel - persistent infection cull area
91
17363
6.3%
900
5.2%
15529
89.4%
1196
6.9 %
927
5.3 %
7
0.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
811 103112
37.3%
3213
3.1%
92983
90.2%
7322
7.1 %
5741
5.6 %
1193
1.2 %
Parallel - new infection cull area
2
298
0.1%
21
7.0%
258
86.6%
21
7.0 %
19
6.4 %
0
Parallel Persistent Infection
18
2026
0.7%
54
2.7%
1879
92.7%
114
5.6 %
90
4.4 %
3
0.1 %
Serial - Suspected NSR Herd
3
11
0.0%
0
0.0 %
11
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
79
9364
3.4%
264
2.8%
8274
88.4%
953
10.2 %
788
8.4 %
38
0.4 %
Parallel Persistent Infection
7
893
0.3%
7
0.8%
704
78.8%
108
12.1 %
89
10.0 %
93
10.4 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

England
1747 221109
79.9%
8632
3.9%
197291
89.2%
16244
7.3 %
12684
5.7 %
2521
1.1 %
Wales - High TB
Extended Flexible IFN-GAMMA test
111
2929
1.1%
205
7.0%
2620
89.5%
134
4.6 %
101
3.4 %
3
0.1 %
Parallel - Herd or Group Slaughter
132
7184
2.6%
409
5.7%
6429
89.5%
457
6.4 %
336
4.7 %
11
0.2 %
Parallel New Infection
37
1419
0.5%
81
5.7%
1280
90.2%
67
4.7 %
57
4.0 %
1
0.1 %
Parallel - Other
40
1886
0.7%
138
7.3%
1646
87.3%
136
7.2 %
102
5.4 %
0
Parallel Persistent Infection
285
24009
8.7%
1005
4.2%
21860
91.0%
1431
6.0 %
1117
4.7 %
29
0.1 %
Parallel - Rapid Testing Two-times IRs
323
981
0.4%
197
20.1%
695
70.8%
70
7.1 %
50
5.1 %
39
4.0 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
47
1118
0.4%
96
8.6%
921
82.4%
59
5.3 %
48
4.3 %
53
4.7 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
88
7534
2.7%
233
3.1%
6871
91.2%
488
6.5 %
375
5.0 %
55
0.7 %
Parallel - Other
20
586
0.2%
51
8.7%
498
85.0%
44
7.5 %
37
6.3 %
0
Parallel Persistent Infection
22
2957
1.1%
109
3.7%
2743
92.8%
164
5.5 %
114
3.9 %
0
Parallel - Rapid Testing Two-times IRs
45
264
0.1%
25
9.5%
231
87.5%
8
3.0 %
8
3.0 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
30
1265
0.5%
30
2.4%
1162
91.9%
87
6.9 %
61
4.8 %
16
1.3 %
Parallel - Other
3
35
0.0%
1
2.9%
32
91.4%
4
11.4 %
2
5.7 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
4
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Wales
1197
52791
19.1%
2608
4.9%
47556
90.1%
3183
6.0 %
2436
4.6 %
207
0.4 %
Scotland - Scotland
Parallel New Infection
19
1998
0.7%
81
4.1%
1815
90.8%
112
5.6 %
92
4.6 %
10
0.5 %
Parallel Persistent Infection
1
338
0.1%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Parallel - persistent infection cull area
1
352
0.1%
7
2.0%
340
96.6%
8
2.3 %
5
1.4 %
0
Scotland
21
2688
1.0%
90
3.3%
2484
92.4%
127
4.7 %
104
3.9 %
10
0.4 %
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2018         | November |
| December     | 228      |
| 2019         | January  |
| February     | 320      |
| March        | 315      |
| April        | 269      |
| May          | 364      |
| June         | 287      |
| July         | 316      |
| August       | 262      |
| September    | 245      |
| October      | 298      |
| Totals (last |          |
| 12 months)   |          |
| 3475         | 322712   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2028
254739
1313
10534
221
0
0
0
0
954
12
2
44
5
9090
8
97
0
2
Scotland
21
2688
11
90
0
0
0
0
0
12
0
0
2
0
70
0
5
0
0
Wales
1426
65285
721
3527
84
0
0
0
0
150
6
3
17
3
2440
81
632
2
21
Totals
3475
322712
2045
14151
305
0
0
0
0
1116
18
5
63
8
11600
89
734
2
23

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Bucks         | 2.30 %   | 3.07 %                 |
| Cambs         | 27.27 %  | 27.27 %                |
| Carms         | 4.16 %   | 5.89 %                 |
| Ceredigion    | 7.02 %   | 8.77 %                 |
| Cheshire      | 3.20 %   | 3.77 %                 |
| Corn & Scilly | 3.34 %   | 3.65 %                 |
| Cumbria       | 11.15 %  | 14.18 %                |
| Derbyshire    | 4.70 %   | 5.99 %                 |
| Devon         | 6.44 %   | 8.78 %                 |
| Dorset        | 6.12 %   | 7.71 %                 |
| Dumfries & G  | 9.65 %   | 11.40 %                |
| Gloucs        | 3.01 %   | 3.71 %                 |
| Gwent         | 3.11 %   | 4.35 %                 |
| Gwynedd       | 7.26 %   | 9.93 %                 |
| Hampshire     | 7.14 %   | 7.14 %                 |
| Heref         | 4.12 %   | 5.76 %                 |
| Kirkcudbright | 2.24 %   | 3.08 %                 |
| Leics & Rut   | 2.36 %   | 3.24 %                 |
| NE Wales      | 6.18 %   | 8.46 %                 |
| Northants     | 2.15 %   | 3.23 %                 |
| Northumb      | 3.30 %   | 3.30 %                 |
| Oxon          | 8.21 %   | 10.02 %                |
| Pembs         | 1.99 %   | 3.40 %                 |
| Powys           | 3.53 %   | 4.51 %   |
|-----------------|----------|----------|
| S Glamorgan     |          |          |
| Shropshire      | 8.24 %   | 9.41 %   |
| Somerset excl N | 6.94 %   | 6.94 %   |
| Suffolk         | 12.42 %  | 13.42 %  |
| Warks           | 4.07 %   | 5.20 %   |
| W Glamorgan     | 6.96 %   | 9.57 %   |
| Wiltshire       | 5.55 %   | 6.51 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
19686
483
2.5%
20315
483
2.4%
Wales
5803
253
4.4%
5904
253
4.3%
Scotland
447
7
1.6%
471
7
1.5%
GB
25936
743
2.9%
26690
743
2.8%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
19127
1054
5.5%
19262
1054
5.5%
Wales
4632
362
7.8%
4699
363
7.7%
Scotland
14
0
0.0 %
15
0.0 %
GB
23773
1416
6.0%
23976
1417
5.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
195986
8632
4.4%
221109
8632
3.9%
Wales
48299
2608
5.4%
52791
2608
4.9%
Scotland
2010
90
4.5%
2688
90
3.3%
GB
246291
11330
4.6%
276588
11330
4.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
140798
7718
5.5%
160008
7718
4.8%
Wales
39468
1949
4.9%
45113
1950
4.3%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
182329
9848
5.4%
207362
9849
4.7%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Devon
PRV-SE
1
1
0
0
PRV-SP
2
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SP
2
5
0
0
Total
6
9
0
Isle of Man
SER-IOM
1
4
0
0
* Test type
Num Submissions
Devon
PRV-SE
2
7
0
0
PRV-SP
3
3
0
0
Dorset
PRV-SP
1
2
0
0
Gloucestershire
PRV-SP
1
4
0
0
Herefordshire
PRV-SP
1
2
0
0
Northern Somerset & South Glou
PRV-SP
1
1
0
0
Somerset excl North
PRV-SE
6
247
16
6.48 %
PRV-SP
5
8
0
0
Staffordshire
PRV-SE
1
64
3
4.69 %
Wiltshire
PRV-SP
1
3
0
0
Worcestershire
SER-OTHER
1
5
0
0
Total
23
346
19
5.49 %
Isle of Man
SER-IOM
26
368
2
0.54 %

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

Private
% Positive
% Positive
Num Samples
Num Positives Private
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |                57 | 13                   | 22.8%                             |
| North East Wales | 3               |       38 |                 2 | 5.3%                 |                                   |
| Pembrokeshire    | 3               |       72 |                 3 | 4.2%                 |                                   |
| Powys            | 2               |       54 |                 6 | 11.1%                |                                   |
| TC0077           | Sum:            |      221 |                24 | 10.9 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 15       |               563 | 52                   | 9.2%                              |
| Ceredigion       | 3               | 372      |                17 | 4.6%                 |                                   |
| North East Wales | 27              | 896      |               116 | 12.9%                |                                   |
| Pembrokeshire    | 19              | 618      |                35 | 5.7%                 |                                   |
| Powys            | 8               | 367      |                24 | 6.5%                 |                                   |
| South Wales      | 5               | 196      |                16 | 8.2%                 |                                   |
| Wiltshire        | 1               | 248      |                 9 | 3.6%                 |                                   |
| TC0077           | Sum:            | 3,260    |               269 | 8.3 %                |                                   |