# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 163

# Report Period 1St - 31St March 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |

## Table 1. Monthly Totals For March 2020

| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| March 2020              |                          |          |
| Submissions             | 262                      | 928      |
| Samples                 | Total (%)                | 18221    |
| England                 | 14090                    | 77.33 %  |
| Wales                   | 3363                     | 18.46 %  |
| Scotland                | 768                      | 4.21 %   |
| Parallel Tests          | PAR-CUL-N                | 6295     |
| PAR-CUL-P               | 842                      | 4.62 %   |
| PAR-HERD-S              | 53                       | 0.29 %   |
| PAR-NEW-IN              | 9083                     | 49.85 %  |
| PAR-OTHER               | 74                       | 0.41 %   |
| PAR-PERSIS              | 1539                     | 8.45 %   |
| PAR-RAPID               | 134                      | 0.74 %   |
| Total(% of all samples) | 18020                    | 98.90 %  |
| Serial/Extended Tests   | SER-FLEXI                | 201      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 201                      | 1.10 %   |
| Total                   | 18221                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1217     |
| England                 | 882                      | 4.84 %   |
| Wales                   | 285                      | 1.56 %   |
| Scotland                | 50                       | 0.27 %   |
| Resamples               | Total (% of all samples) | 961      |
| England                 | 699                      | 3.84 %   |
| Wales                   | 217                      | 1.19 %   |
| Scotland                | 45                       | 0.25 %   |
| Rejects                 | Total (% of all samples) | 271      |
| England                 | 263                      | 1.44 %   |
| Wales                   | 8                        | 0.04 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   6295 | 34.5 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    842 | 4.6 %    |
| PAR-HERD-S  |     53 | 0.3 %    |
| PAR-NEW-IN  |   9083 | 49.8 %   |
| PAR-OTHER   |     74 | 0.4 %    |
| PAR-PERSIS  |   1539 | 8.4 %    |
| PAR-RAPID   |    134 | 0.7 %    |
| SER-FLEXI   |    201 | 1.1 %    |
| Total       |  18221 | 100.00 % |
Breakdown
of Samples
submitted
by County

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For March 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 1              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 1              |
| Dorset          | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| Gloucs          | PAR-CUL-N      |
| Shropshire      | PAR-CUL-N      |
| PAR-PERSIS      | 1              |
| Somerset excl N | PAR-CUL-N      |
| PAR-PERSIS      | 2              |
| Staffs          | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 1              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 17             |
| Derbyshire      | PAR-NEW-IN     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| Leics & Rut     | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Gr Manchester  |
| Humberside      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| Surrey          | PAR-NEW-IN     |
| England         |                |
| 139             | 14090          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 7              |
| PAR-RAPID       | 6              |
| SER-FLEXI       | 1              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 5              |
| PAR-RAPID       | 2              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 4              |
| Pembs           | PAR-OTHER      |
| PAR-PERSIS      | 19             |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 1              |
| Powys           | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 4              |
| SER-FLEXI       | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| W Glamorgan             | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |
| Ceredigion              | SER-FLEXI      |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-NEW-IN     |
| Wales - Low TB          | Gwynedd        |
| PAR-OTHER               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |
| Wales                   |                |
| 113                     | 3363           |
| Scotland - Scotland     | Argyll & Bute  |
| Berwickshire            | PAR-NEW-IN     |
| Dumfries & G            | PAR-NEW-IN     |
| Scotland                |                |
| 10                      | 768            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 4              |
| PAR-PERSIS      | 16             |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 7              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| PAR-NEW-IN      | 2              |
| SER-FLEXI       | 5              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-CUL-N      |
| PAR-PERSIS      | 7              |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 2              |
| Staffs          | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-PERSIS      | 2              |
| SER-FLEXI       | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 59             |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 8              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Gr Manchester   | PAR-NEW-IN     |
| Humberside      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| Surrey          | PAR-NEW-IN     |
| England         |                |
| 548             | 65835          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 11             |
| PAR-PERSIS      | 21             |
| PAR-RAPID       | 19             |
| SER-FLEXI       | 2              |
| Ceredigion      | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 15             |
| PAR-RAPID               | 7              |
| Gwent                   | PAR-HERD-S     |
| PAR-PERSIS              | 13             |
| PAR-RAPID               | 9              |
| SER-FLEXI               | 3              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 24             |
| PAR-PERSIS              | 47             |
| PAR-RAPID               | 23             |
| SER-FLEXI               | 3              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 10             |
| PAR-OTHER               | 4              |
| PAR-PERSIS              | 20             |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 9              |
| S Wales                 | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| PAR-PERSIS              | 2              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 3              |
| Ceredigion              | PAR-PERSIS     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-RAPID           | 1              |
| SER-FLEXI           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 10             |
| PAR-RAPID           | 5              |
| SER-FLEXI           | 5              |
| Powys               | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| S Glamorgan         | SER-FLEXI      |
| Wales - Low TB      | Gwynedd        |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| Wales               |                |
| 359                 | 14606          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Berwickshire        | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 21                  | 2200           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 139          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 10           |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 113          |
| GB              | Parallel     |
| Serial/Extended | 10           |
| Total           | 262          |
| 139.00          |              |
| 113.00          |              |
| 10.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 13           |
| Total           | 548          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 21           |
| Wales           | Parallel     |
| Serial/Extended | 29           |
| Total           | 359          |
| GB              | Parallel     |
| Serial/Extended | 42           |
| Total           | 928          |
| 548.00          |              |
| 359.00          |              |
| 21.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
10
201
1.1%
21
10.4%
169
84.1%
22
10.9 %
9
4.5 %
2
1.0 %
Parallel - Herd or Group Slaughter
7
53
0.3%
7
13.2%
44
83.0%
4
7.5 %
2
3.8 %
0
Parallel New Infection
89
9083
49.8%
276
3.0%
8192
90.2%
607
6.7 %
510
5.6 %
105
1.2 %
Parallel - new infection cull area
66
6295
34.5%
287
4.6%
5549
88.1%
398
6.3 %
300
4.8 %
159
2.5 %
Parallel - Other
13
74
0.4%
7
9.5%
64
86.5%
4
5.4 %
3
4.1 %
0
Parallel Persistent Infection
50
1539
8.4%
123
8.0%
1321
85.8%
126
8.2 %
90
5.8 %
5
0.3 %
Parallel - persistent infection cull area
4
842
4.6%
18
2.1%
784
93.1%
47
5.6 %
40
4.8 %
0
Parallel - Rapid Testing Two-times IRs
23
134
0.7%
25
18.7%
102
76.1%
9
6.7 %
7
5.2 %
0
Total
262
18221
100.0%
764
4.2%
16225
89.0%
1217
6.7 %
961
5.3 %
271
1.5 %
England - HRA
Parallel New Infection
1
6
0.0%
0
0.0 %
6
100.0%
1
16.7 %
0
0
Parallel - new infection cull area
63
6242
34.3%
286
4.6%
5507
88.2%
388
6.2 %
292
4.7 %
157
2.5 %
Parallel Persistent Infection
6
412
2.3%
34
8.3%
358
86.9%
28
6.8 %
20
4.9 %
0
Parallel - persistent infection cull area
4
842
4.6%
18
2.1%
784
93.1%
47
5.6 %
40
4.8 %
0
England - HRA
74
7502
41.2%
338
4.5%
6655
88.7%
464
6.2 %
352
4.7 %
157
2.1 %
England - Edge
Parallel New Infection
52
6123
33.6%
173
2.8%
5523
90.2%
388
6.3 %
323
5.3 %
104
1.7 %
Parallel - new infection cull area
3
53
0.3%
1
1.9%
42
79.2%
10
18.9 %
8
15.1 %
2
3.8 %
England - Edge
55
6176
33.9%
174
2.8%
5565
90.1%
398
6.4 %
331
5.4 %
106
1.7 %
England - LRA
Parallel New Infection
10
412
2.3%
21
5.1%
375
91.0%
20
4.9 %
16
3.9 %
0
England - LRA
10
412
2.3%
21
5.1%
375
91.0%
20
4.9 %
16
3.9 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
5
105
0.6%
17
16.2%
86
81.9%
11
10.5 %
2
1.9 %
0
Parallel - Herd or Group Slaughter
4
23
0.1%
2
8.7%
20
87.0%
1
4.3 %
1
4.3 %
0
Parallel New Infection
1
8
0.0%
2
25.0%
6
75.0%
0
0
0
Parallel - Other
11
58
0.3%
5
8.6%
51
87.9%
2
3.4 %
2
3.4 %
0
Parallel Persistent Infection
39
757
4.2%
56
7.4%
642
84.8%
75
9.9 %
54
7.1 %
5
0.7 %
Parallel - Rapid Testing Two-times IRs
21
118
0.6%
24
20.3%
89
75.4%
7
5.9 %
5
4.2 %
0
Wales - High TB
81
1069
5.9%
106
9.9%
894
83.6%
96
9.0 %
64
6.0 %
5
0.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
96
0.5%
4
4.2%
83
86.5%
11
11.5 %
7
7.3 %
2
2.1 %
Parallel - Herd or Group Slaughter
3
30
0.2%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
9
1331
7.3%
33
2.5%
1185
89.0%
120
9.0 %
113
8.5 %
0
Parallel - Other
1
15
0.1%
2
13.3%
13
86.7%
1
6.7 %
0
0
Parallel Persistent Infection
4
330
1.8%
33
10.0%
289
87.6%
13
3.9 %
8
2.4 %
0
Parallel - Rapid Testing Two-times IRs
2
16
0.1%
1
6.3%
13
81.3%
2
12.5 %
2
12.5 %
0
Wales - Intermediate TB
24
1818
10.0%
78
4.3%
1607
88.4%
150
8.3 %
131
7.2 %
2
0.1 %
Wales - Low TB
Parallel New Infection
6
435
2.4%
5
1.1%
416
95.6%
28
6.4 %
13
3.0 %
1
0.2 %
Parallel - Other
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel Persistent Infection
1
40
0.2%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Wales - Low TB
8
476
2.6%
5
1.1%
448
94.1%
39
8.2 %
22
4.6 %
1
0.2 %
Scotland - Scotland
Parallel New Infection
10
768
4.2%
42
5.5%
681
88.7%
50
6.5 %
45
5.9 %
0
Scotland - Scotland
10
768
4.2%
42
5.5%
681
88.7%
50
6.5 %
45
5.9 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
42
1857
2.2%
128
6.9%
1664
89.6%
102
5.5 %
63
3.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
18
279
0.3%
27
9.7%
243
87.1%
20
7.2 %
9
3.2 %
0
Parallel New Infection
317
36750
44.5%
1047
2.8%
32474
88.4%
2383
6.5 %
1951
5.3 %
1278
3.5 %
Parallel - new infection cull area
241
27583
33.4%
1116
4.0%
24686
89.5%
1699
6.2 %
1322
4.8 %
459
1.7 %
Parallel - Other
49
911
1.1%
96
10.5%
761
83.5%
67
7.4 %
54
5.9 %
0
Parallel Persistent Infection
165
11047
13.4%
682
6.2%
9794
88.7%
719
6.5 %
556
5.0 %
15
0.1 %
Parallel - persistent infection cull area
21
3915
4.7%
259
6.6%
3479
88.9%
239
6.1 %
176
4.5 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
75
299
0.4%
65
21.7%
222
74.2%
14
4.7 %
12
4.0 %
0
Total
928
82641
100.0%
3420
4.1%
73323
88.7%
5243
6.3 %
4143
5.0 %
1755
2.1 %
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.5%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
5
415
0.5%
15
3.6%
363
87.5%
49
11.8 %
36
8.7 %
1
0.2 %
Parallel - new infection cull area
237
27169
32.9%
1100
4.0%
24317
89.5%
1650
6.1 %
1295
4.8 %
457
1.7 %
Parallel Persistent Infection
30
4958
6.0%
241
4.9%
4502
90.8%
284
5.7 %
212
4.3 %
3
0.1 %
Parallel - persistent infection cull area
21
3915
4.7%
259
6.6%
3479
88.9%
239
6.1 %
176
4.5 %
1
0.0 %
England - Edge
Parallel New Infection
218
26497
32.1%
779
2.9%
23508
88.7%
1639
6.2 %
1320
5.0 %
890
3.4 %
Parallel - new infection cull area
4
414
0.5%
16
3.9%
369
89.1%
49
11.8 %
27
6.5 %
2
0.5 %
England - LRA
Parallel New Infection
20
1243
1.5%
35
2.8%
856
68.9%
44
3.5 %
38
3.1 %
314
25.3 %
England
548
65835
79.7%
2515
3.8%
58507
88.9%
4013
6.1 %
3145
4.8 %
1668
2.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
19
453
0.5%
42
9.3%
398
87.9%
30
6.6 %
13
2.9 %
0
Parallel - Herd or Group Slaughter
15
249
0.3%
22
8.8%
219
88.0%
17
6.8 %
8
3.2 %
0
Parallel New Infection
11
1281
1.6%
52
4.1%
1089
85.0%
148
11.6 %
133
10.4 %
7
0.5 %
Parallel - Other
45
882
1.1%
94
10.7%
737
83.6%
62
7.0 %
51
5.8 %
0
Parallel Persistent Infection
119
5367
6.5%
353
6.6%
4690
87.4%
396
7.4 %
312
5.8 %
12
0.2 %
Parallel - Rapid Testing Two-times IRs
65
271
0.3%
59
21.8%
202
74.5%
12
4.4 %
10
3.7 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
10
180
0.2%
16
8.9%
153
85.0%
13
7.2 %
9
5.0 %
2
1.1 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel New Infection
30
3764
4.6%
83
2.2%
3437
91.3%
277
7.4 %
244
6.5 %
0
Parallel - Other
3
28
0.0%
2
7.1%
24
85.7%
4
14.3 %
2
7.1 %
0
Parallel Persistent Infection
15
682
0.8%
88
12.9%
570
83.6%
29
4.3 %
24
3.5 %
0
Parallel - Rapid Testing Two-times IRs
9
27
0.0%
6
22.2%
19
70.4%
2
7.4 %
2
7.4 %
0
Wales - Low TB
Parallel New Infection
12
1350
1.6%
17
1.3%
1264
93.6%
97
7.2 %
68
5.0 %
1
0.1 %
Parallel - Other
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales
359
14606
17.7%
839
5.7%
12859
88.0%
1101
7.5 %
886
6.1 %
22
0.2 %
Scotland - Scotland
Parallel New Infection
21
2200
2.7%
66
3.0%
1957
89.0%
129
5.9 %
112
5.1 %
65
3.0 %
Scotland
21
2200
2.7%
66
3.0%
1957
89.0%
129
5.9 %
112
5.1 %
65
3.0 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | April   |
| May          | 364     |
| June         | 287     |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| 2020         | January |
| February     | 323     |
| March        | 262     |
| Totals (last |         |
| 12 months)   |         |
| 3475         | 329426  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
2071
261990
1270
8940
316
0
0
0
0
1069
8
6
42
2
7385
7
62
0
1
Scotland
40
4527
21
152
32
0
0
0
0
13
0
0
3
0
94
8
1
0
0
Wales
1364
62909
694
2906
72
0
0
0
0
115
8
8
23
1
2070
118
478
0
6
Totals
3475
329426
1985
11998
420
0
0
0
0
1197
16
14
68
3
9549
133
541
0
7

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Argyll & Bute | 6.10 %   | 7.32 %                 |
| Berks         | 7.02 %   | 8.06 %                 |
| Berwickshire  | 10.26 %  | 10.26 %                |
| Bucks         | 4.00 %   | 6.15 %                 |
| Carms         | 4.76 %   | 10.12 %                |
| Ceredigion    | 2.50 %   | 2.50 %                 |
| Cheshire      | 5.22 %   | 5.98 %                 |
| Corn & Scilly | 4.49 %   | 6.36 %                 |
| Derbyshire    | 7.85 %   | 8.99 %                 |
| Devon         | 4.15 %   | 5.76 %                 |
| Dorset        | 6.56 %   | 8.13 %                 |
| Dumfries & G  | 4.29 %   | 4.29 %                 |
| Gloucs        | 3.60 %   | 4.63 %                 |
| Gr Manchester | 6.90 %   | 6.90 %                 |
| Gwent         | 7.53 %   | 11.83 %                |
| Gwynedd       | 4.65 %   | 6.98 %                 |
| Hampshire     | 2.99 %   | 3.42 %                 |
| Humberside    | 6.41 %   | 8.97 %                 |
| Leics & Rut   | 3.35 %   | 5.93 %                 |
| Lincs         | 1.69 %   | 1.69 %                 |
| NE Wales      | 6.93 %   | 8.29 %                 |
| Norfolk       |          |                        |
| N Yorks       |          |                        |
| Oxon            | 7.44 %   | 7.66 %   |
|-----------------|----------|----------|
| Pembs           | 6.84 %   | 8.51 %   |
| Powys           | 4.17 %   | 11.11 %  |
| Shropshire      | 3.94 %   | 5.56 %   |
| Somerset excl N | 3.08 %   | 4.44 %   |
| Staffs          | 4.51 %   | 4.66 %   |
| Surrey          | 3.98 %   | 5.11 %   |
| Warks           | 2.36 %   | 3.34 %   |
| W Glamorgan     | 9.09 %   |          |
| Wiltshire       | 11.83 %  | 13.61 %  |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
13849
533
3.8%
14090
533
3.8%
Wales
3282
189
5.8%
3363
189
5.6%
Scotland
751
42
5.6%
768
42
5.5%
GB
17882
764
4.3%
18221
764
4.2%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23913
1241
5.2%
24319
1241
5.1%
Wales
4862
301
6.2%
4939
301
6.1%
Scotland
366
4
1.1%
366
4
1.1%
GB
29141
1546
5.3%
29624
1546
5.2%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
62705
2515
4.0%
65835
2515
3.8%
Wales
13942
838
6.0%
14606
839
5.7%
Scotland
2072
66
3.2%
2200
66
3.0%
GB
78719
3419
4.3%
82641
3420
4.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
64280
3601
5.6%
67619
3601
5.3%
Wales
16382
1131
6.9%
17196
1131
6.6%
Scotland
366
4
1.1%
366
4
1.1%
GB
81028
4736
5.8%
85181
4736
5.6%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
| % Positive      | * Test type   |
|-----------------|---------------|
| Num             |               |
| Submissions     |               |
| Num             |               |
| Samples         |               |
| Num             |               |
| Positives       |               |
| Private         |               |
| Cheshire        | PRV-SP        |
| Devon           | PRV-SE        |
| PRV-SP          | 1             |
| Dorset          | PRV-SE        |
| PRV-SP          | 1             |
| Gloucestershire | PRV-SE        |
| Oxfordshire     | PRV-SP        |
| Staffordshire   | PRV-SE        |
| Worcestershire  | PRV-SP        |
| Total           |               |
| 9               |               |
| 195             |               |
| 3               | 1.54 %        |
| Isle of Man     | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |                33 | 6                    | 18.2%                             |
| Ceredigion       | 3               |        5 |                 1 | 20.0%                |                                   |
| North East Wales | 6               |      370 |                36 | 9.7%                 |                                   |
| Pembrokeshire    | 7               |      120 |                23 | 19.2%                |                                   |
| Powys            | 2               |       83 |                11 | 13.3%                |                                   |
| South Wales      | 4               |       97 |                26 | 26.8%                |                                   |
| TC0077           | 24              |      708 |               103 | 14.5 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 8        |               216 | 32                   | 14.8%                             |
| Ceredigion       | 4               | 7        |                 1 | 14.3%                |                                   |
| Devon            | 4               | 1,032    |                95 | 9.2%                 |                                   |
| North East Wales | 12              | 505      |                54 | 10.7%                |                                   |
| Pembrokeshire    | 16              | 357      |                56 | 15.7%                |                                   |
| Powys            | 2               | 83       |                11 | 13.3%                |                                   |
| South Wales      | 8               | 142      |                30 | 21.1%                |                                   |
| TC0077           | 54              | 2,342    |               279 | 11.9 %               |                                   |