# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 146

# Report Period 1St - 31St October 2018

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2018               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| October                 |                          |          |
| 2018                    |                          |          |
| Submissions             | 274                      | 2159     |
| Samples                 | Total (%)                | 23976    |
| England                 | 19262                    | 80.34 %  |
| Wales                   | 4699                     | 19.60 %  |
| Scotland                | 15                       | 0.06 %   |
| Parallel Tests          | PAR-CUL-N                | 4511     |
| PAR-CUL-P               | 596                      | 2.49 %   |
| PAR-HERD-S              | 423                      | 1.76 %   |
| PAR-NEW-IN              | 11297                    | 47.12 %  |
| PAR-OTHER               | 1235                     | 5.15 %   |
| PAR-PERSIS              | 4932                     | 20.57 %  |
| PAR-RAPID               | 163                      | 0.68 %   |
| Total(% of all samples) | 23157                    | 96.58 %  |
| Serial/Extended Tests   | SER-FLEXI                | 809      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 10                       | 0.04 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 819                      | 3.42 %   |
| Total                   | 23976                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1860     |
| England                 | 1562                     | 6.51 %   |
| Wales                   | 297                      | 1.24 %   |
| Scotland                | 1                        | 0.00 %   |
| Resamples               | Total (% of all samples) | 1145     |
| England                 | 974                      | 4.06 %   |
| Wales                   | 170                      | 0.71 %   |
| Scotland                | 1                        | 0.00 %   |
| Rejects                 | Total (% of all samples) | 56       |
| England                 | 11                       | 0.05 %   |
| Wales                   | 45                       | 0.19 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   4511 | 18.8 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    596 | 2.5 %    |
| PAR-HERD-S  |    423 | 1.8 %    |
| PAR-NEW-IN  |  11297 | 47.1 %   |
| PAR-OTHER   |   1235 | 5.2 %    |
| PAR-PERSIS  |   4932 | 20.6 %   |
| PAR-RAPID   |    163 | 0.7 %    |
| SER-FLEXI   |    809 | 3.4 %    |
| SER-NSR     |     10 | 0.0 %    |
| Total       |  23976 | 100.00 % |
Breakdown
of Samples
submitted
by County
| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Avon           |
| Corn & Scilly  | PAR-CUL-N      |
| PAR-PERSIS     | 1              |
| Devon          | PAR-CUL-N      |
| PAR-CUL-P      | 1              |
| PAR-NEW-IN     | 2              |
| PAR-PERSIS     | 3              |
| Dorset         | PAR-CUL-N      |
| PAR-CUL-P      | 1              |
| PAR-OTHER      | 2              |
| SER-FLEXI      | 1              |
| Gloucs         | PAR-CUL-N      |
| PAR-CUL-P      | 3              |
| PAR-PERSIS     | 4              |
| SER-FLEXI      | 2              |
| Heref          | PAR-CUL-N      |
| PAR-PERSIS     | 1              |
| Shropshire     | PAR-OTHER      |
| Somerset       | PAR-CUL-N      |
| Wiltshire      | PAR-NEW-IN     |
| PAR-PERSIS     | 3              |
| SER-FLEXI      | 2              |
| England - Edge | Berks          |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| PAR-PERSIS     | 1              |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For October 2018 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Hampshire       | PAR-NEW-IN     |
| SER-NSR         | 1              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Kent            | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| England         |                |
| 157             | 19262          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 8              |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 4              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 1              |
| PAR-RAPID       | 3              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 2              |
| Pembs           | PAR-HERD-S     |
| PAR-PERSIS      | 10             |
| PAR-RAPID       | 18             |
| SER-FLEXI       | 10             |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 4              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For October 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 5              |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Ceredigion     |
| SER-FLEXI               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-NEW-IN     |
| Shropshire              | PAR-NEW-IN     |
| W Glamorgan             | PAR-OTHER      |
| Wales - Low TB          | NE Wales       |
| PAR-RAPID               | 1              |
| Wales                   |                |
| 115                     | 4699           |
| Scotland - Scotland     | Aberdeenshire  |
| Scotland                |                |
| 2                       | 15             |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-PERSIS    | 8              |
| SER-FLEXI     | 2              |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 1              |
| PAR-OTHER     | 3              |
| PAR-PERSIS    | 22             |
| SER-FLEXI     | 9              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 18             |
| PAR-NEW-IN    | 9              |
| PAR-OTHER     | 5              |
| PAR-PERSIS    | 34             |
| SER-FLEXI     | 14             |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 13             |
| SER-FLEXI     | 1              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 9              |
| PAR-NEW-IN    | 2              |
| PAR-OTHER     | 2              |
| PAR-PERSIS    | 4              |
| SER-FLEXI     | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| Heref          | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-NEW-IN     | 3              |
| PAR-PERSIS     | 4              |
| SER-FLEXI      | 2              |
| SER-FRAUD      | 2              |
| Shropshire     | PAR-OTHER      |
| PAR-PERSIS     | 12             |
| Somerset       | PAR-CUL-N      |
| PAR-CUL-P      | 1              |
| PAR-NEW-IN     | 1              |
| PAR-OTHER      | 5              |
| PAR-PERSIS     | 21             |
| Staffs         | PAR-PERSIS     |
| SER-FLEXI      | 2              |
| Wiltshire      | PAR-NEW-IN     |
| PAR-OTHER      | 2              |
| PAR-PERSIS     | 11             |
| SER-FLEXI      | 2              |
| SER-FRAUD      | 2              |
| W Midlands     | PAR-PERSIS     |
| SER-FRAUD      | 3              |
| England - Edge | Berks          |
| PAR-PERSIS     | 6              |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| PAR-PERSIS     | 10             |
| Derbyshire     | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| E Sussex      | PAR-NEW-IN     |
| Hampshire     | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 1              |
| SER-NSR       | 3              |
| Leics & Rut   | PAR-NEW-IN     |
| SER-NSR       | 1              |
| Northants     | PAR-NEW-IN     |
| Notts         | PAR-NEW-IN     |
| PAR-OTHER     | 1              |
| Oxon          | PAR-NEW-IN     |
| PAR-PERSIS    | 8              |
| Warks         | PAR-NEW-IN     |
| PAR-PERSIS    | 2              |
| England - LRA | Beds           |
| Cumbria       | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| Essex         | PAR-NEW-IN     |
| Herts         | PAR-NEW-IN     |
| Humberside    | PAR-NEW-IN     |
| Isle of wight | PAR-NEW-IN     |
| Kent          | PAR-NEW-IN     |
| Lincs         | PAR-NEW-IN     |
| Merseyside    | PAR-NEW-IN     |
| Norfolk       | PAR-NEW-IN     |
| N Yorks       | PAR-NEW-IN     |
| S Yorks       | PAR-NEW-IN     |
| W Sussex      | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2018 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-PERSIS      | 2              |
| England         |                |
| 1188            | 160008         |
| Wales - High TB | Carms          |
| PAR-OTHER       | 6              |
| PAR-PERSIS      | 42             |
| PAR-RAPID       | 85             |
| SER-FLEXI       | 10             |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 16             |
| PAR-RAPID       | 28             |
| SER-FLEXI       | 8              |
| Gwent           | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 37             |
| PAR-RAPID       | 32             |
| SER-FLEXI       | 6              |
| Pembrokshire    | PAR-OTHER      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 77             |
| PAR-RAPID       | 121            |
| SER-FLEXI       | 24             |
| SER-FRAUD       | 2              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 22             |
| PAR-OTHER       | 5              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 43             |
| PAR-RAPID               | 31             |
| SER-FLEXI               | 6              |
| S Wales                 | PAR-PERSIS     |
| PAR-RAPID               | 3              |
| W Glamorgan             | PAR-RAPID      |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 2              |
| Ceredigion              | PAR-HERD-S     |
| PAR-NEW-IN              | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 19             |
| SER-FLEXI               | 4              |
| Mid glamorgan           | PAR-PERSIS     |
| NE Wales                | PAR-HERD-S     |
| PAR-NEW-IN              | 115            |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 9              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 8              |
| PAR-RAPID               | 2              |
| Shropshire              | PAR-NEW-IN     |
| W Glamorgan             | PAR-OTHER      |
| SER-FLEXI               | 2              |
| Wales - Low TB          | Gwynedd        |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2018 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 3              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 940                 | 45113          |
| Scotland - Scotland | Aberdeenshire  |
| Argyll & Bute       | PAR-NEW-IN     |
| SER-FLEXI           | 1              |
| Ayrshire            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| Perthshire          | PAR-NEW-IN     |
| West lothian        | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 31                  | 2241           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol October 2018

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 6            |
| Total           | 157          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 2            |
| Wales           | Parallel     |
| Serial/Extended | 21           |
| Total           | 115          |
| GB              | Parallel     |
| Serial/Extended | 27           |
| Total           | 274          |
| 157.00          |              |
| 115.00          |              |
| 2.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2018)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 49           |
| Total           | 1188 160008  |
| Scotland        | Parallel     |
| Serial/Extended | 1            |
| Total           | 31           |
| Wales           | Parallel     |
| Serial/Extended | 68           |
| Total           | 940          |
| GB              | Parallel     |
| Serial/Extended | 118          |
| Total           | 2159 207362  |

#######
940.00
31.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (October 2018)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
26
809
3.4%
72
8.9%
701
86.7%
48
5.9 %
30
3.7 %
6
0.7 %
Parallel - Herd or Group Slaughter
7
423
1.8%
20
4.7%
392
92.7%
19
4.5 %
11
2.6 %
0
Parallel New Infection
109
11297
47.1%
560
5.0%
10112
89.5%
1016
9.0 %
619
5.5 %
6
0.1 %
Parallel - new infection cull area
37
4511
18.8%
267
5.9%
4007
88.8%
340
7.5 %
230
5.1 %
7
0.2 %
Parallel - Other
9
1235
5.2%
78
6.3%
1108
89.7%
66
5.3 %
48
3.9 %
1
0.1 %
Parallel Persistent Infection
42
4932
20.6%
366
7.4%
4384
88.9%
321
6.5 %
175
3.5 %
7
0.1 %
Parallel - persistent infection cull area
5
596
2.5%
19
3.2%
551
92.4%
41
6.9 %
26
4.4 %
0
Parallel - Rapid Testing Two-times IRs
38
163
0.7%
35
21.5%
93
57.1%
9
5.5 %
6
3.7 %
29
17.8 %
Serial - Suspected NSR Herd
1
10
0.0%
0
0.0 %
10
100.0%
0
0
0
Total
274
23976
100.0%
1417
5.9%
21358
89.1%
1860
7.8 %
1145
4.8 %
56
0.2 %
England - HRA
Extended Flexible IFN-GAMMA test
5
315
1.3%
25
7.9%
281
89.2%
21
6.7 %
8
2.5 %
1
0.3 %
Parallel New Infection
3
891
3.7%
62
7.0%
805
90.3%
44
4.9 %
24
2.7 %
0
Parallel - new infection cull area
37
4511
18.8%
267
5.9%
4007
88.8%
340
7.5 %
230
5.1 %
7
0.2 %
Parallel - Other
7
1215
5.1%
71
5.8%
1095
90.1%
66
5.4 %
48
4.0 %
1
0.1 %
Parallel Persistent Infection
13
2421
10.1%
139
5.7%
2209
91.2%
146
6.0 %
73
3.0 %
0
Parallel - persistent infection cull area
5
596
2.5%
19
3.2%
551
92.4%
41
6.9 %
26
4.4 %
0
England - HRA
70
9949
41.5%
583
5.9%
8948
89.9%
658
6.6 %
409
4.1 %
9
0.1 %
England - Edge
Parallel New Infection
78
8216
34.3%
429
5.2%
7267
88.4%
822
10.0 %
518
6.3 %
2
0.0 %
Parallel Persistent Infection
1
11
0.0%
0
0.0 %
8
72.7%
3
27.3 %
3
27.3 %
0
Serial - Suspected NSR Herd
1
10
0.0%
0
0.0 %
10
100.0%
0
0
0
England - Edge
80
8237
34.4%
429
5.2%
7285
88.4%
825
10.0 %
521
6.3 %
2
0.0 %
England - LRA
Parallel New Infection
7
1076
4.5%
42
3.9%
990
92.0%
79
7.3 %
44
4.1 %
0
England - LRA
7
1076
4.5%
42
3.9%
990
92.0%
79
7.3 %
44
4.1 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
16
410
1.7%
40
9.8%
348
84.9%
21
5.1 %
17
4.1 %
5
1.2 %
Parallel - Herd or Group Slaughter
6
369
1.5%
20
5.4%
341
92.4%
13
3.5 %
8
2.2 %
0
Parallel New Infection
4
163
0.7%
11
6.7%
143
87.7%
12
7.4 %
7
4.3 %
2
1.2 %
Parallel - Other
1
9
0.0%
2
22.2%
7
77.8%
0
0
0
Parallel Persistent Infection
27
2399
10.0%
224
9.3%
2076
86.5%
164
6.8 %
92
3.8 %
7
0.3 %
Parallel - Rapid Testing Two-times IRs
34
153
0.6%
33
21.6%
85
55.6%
9
5.9 %
6
3.9 %
29
19.0 %
Wales - High TB
88
3503
14.6%
330
9.4%
3000
85.6%
219
6.3 %
130
3.7 %
43
1.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
84
0.4%
7
8.3%
72
85.7%
6
7.1 %
5
6.0 %
0
Parallel - Herd or Group Slaughter
1
54
0.2%
0
0.0 %
51
94.4%
6
11.1 %
3
5.6 %
0
Parallel New Infection
12
582
2.4%
12
2.1%
553
95.0%
39
6.7 %
17
2.9 %
0
Parallel - Other
1
11
0.0%
5
45.5%
6
54.5%
0
0
0
Parallel Persistent Infection
1
101
0.4%
3
3.0%
91
90.1%
8
7.9 %
7
6.9 %
0
Parallel - Rapid Testing Two-times IRs
3
9
0.0%
2
22.2%
7
77.8%
0
0
0
Wales - Intermediate TB
23
841
3.5%
29
3.4%
780
92.7%
59
7.0 %
32
3.8 %
0
Wales - Low TB
Parallel New Infection
3
354
1.5%
4
1.1%
340
96.0%
19
5.4 %
8
2.3 %
2
0.6 %
Parallel - Rapid Testing Two-times IRs
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Wales - Low TB
4
355
1.5%
4
1.1%
341
96.1%
19
5.4 %
8
2.3 %
2
0.6 %
Scotland - Scotland
Parallel New Infection
2
15
0.1%
0
0.0 %
14
93.3%
1
6.7 %
1
6.7 %
0
Scotland - Scotland
2
15
0.1%
0
0.0 %
14
93.3%
1
6.7 %
1
6.7 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
104
7801
3.8%
560
7.2%
6761
86.7%
604
7.7 %
396
5.1 %
84
1.1 %
Parallel - Herd or Group Slaughter
89
5696
2.7%
224
3.9%
4711
82.7%
544
9.6 %
391
6.9 %
370
6.5 %
Parallel New Infection
939 109509
52.8%
4118
3.8%
98646
90.1%
9145
8.4 %
6285
5.7 %
460
0.4 %
Parallel - new infection cull area
204
24638
11.9%
1329
5.4%
21775
88.4%
2040
8.3 %
1367
5.5 %
167
0.7 %
Parallel - Other
51
2585
1.2%
225
8.7%
2143
82.9%
147
5.7 %
108
4.2 %
109
4.2 %
Parallel Persistent Infection
388
49364
23.8%
2717
5.5%
43380
87.9%
3687
7.5 %
2665
5.4 %
602
1.2 %
Parallel - persistent infection cull area
32
6155
3.0%
339
5.5%
5428
88.2%
419
6.8 %
288
4.7 %
100
1.6 %
Parallel - Rapid Testing Two-times IRs
338
1315
0.6%
279
21.2%
940
71.5%
83
6.3 %
65
4.9 %
31
2.4 %
Serial - Anomalous Reaction
10
270
0.1%
58
21.5%
204
75.6%
13
4.8 %
8
3.0 %
0
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
Total
2159 207362
100.0%
9849
4.7%
184017
88.7%
16682
8.0 %
11573
5.6 %
1923
0.9 %
England - HRA
Extended Flexible IFN-GAMMA test
37
5876
2.8%
441
7.5%
5190
88.3%
391
6.7 %
233
4.0 %
12
0.2 %
Parallel New Infection
19
2678
1.3%
157
5.9%
2346
87.6%
245
9.1 %
163
6.1 %
12
0.4 %
Parallel - new infection cull area
204
24638
11.9%
1329
5.4%
21775
88.4%
2040
8.3 %
1367
5.5 %
167
0.7 %
Parallel - Other
27
2315
1.1%
161
7.0%
1943
83.9%
138
6.0 %
102
4.4 %
109
4.7 %
Parallel Persistent Infection
133
26852
12.9%
1572
5.9%
23933
89.1%
1843
6.9 %
1291
4.8 %
56
0.2 %
Parallel - persistent infection cull area
32
6155
3.0%
339
5.5%
5428
88.2%
419
6.8 %
288
4.7 %
100
1.6 %
Serial - Anomalous Reaction
8
170
0.1%
3
1.8%
159
93.5%
12
7.1 %
8
4.7 %
0
England - Edge
Parallel New Infection
611
77650
37.4%
3164
4.1%
69852
90.0%
6520
8.4 %
4408
5.7 %
226
0.3 %
Parallel - Other
2
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel Persistent Infection
27
4008
1.9%
210
5.2%
3408
85.0%
334
8.3 %
288
7.2 %
102
2.5 %
Serial - Suspected NSR Herd
4
29
0.0%
0
0.0 %
29
100.0%
0
0
0
England - LRA
Parallel New Infection
81
9008
4.3%
325
3.6%
8135
90.3%
780
8.7 %
545
6.1 %
3
0.0 %
Parallel Persistent Infection
3
627
0.3%
17
2.7%
581
92.7%
33
5.3 %
29
4.6 %
0
England
1188 160008
77.2%
7718
4.8%
142780
89.2%
12756
8.0 %
8723
5.5 %
787
0.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
54
1665
0.8%
108
6.5%
1336
80.2%
196
11.8 %
149
8.9 %
72
4.3 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2018) Cont.

Parallel - Herd or Group Slaughter
79
4787
2.3%
218
4.6%
3976
83.1%
324
6.8 %
224
4.7 %
369
7.7 %
Parallel New Infection
24
869
0.4%
21
2.4%
810
93.2%
52
6.0 %
36
4.1 %
2
0.2 %
Parallel - Other
19
209
0.1%
19
9.1%
185
88.5%
7
3.3 %
5
2.4 %
0
Parallel Persistent Infection
217
17504
8.4%
890
5.1%
15162
86.6%
1412
8.1 %
1008
5.8 %
444
2.5 %
Parallel - Rapid Testing Two-times IRs
302
1175
0.6%
248
21.1%
835
71.1%
79
6.7 %
62
5.3 %
30
2.6 %
Serial - Anomalous Reaction
2
100
0.0%
55
55.0%
45
45.0%
1
1.0 %
0
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
10
204
0.1%
10
4.9%
182
89.2%
14
6.9 %
12
5.9 %
0
Parallel - Herd or Group Slaughter
10
909
0.4%
6
0.7%
735
80.9%
220
24.2 %
167
18.4 %
1
0.1 %
Parallel New Infection
126
11821
5.7%
210
1.8%
10881
92.0%
916
7.7 %
672
5.7 %
58
0.5 %
Parallel - Other
1
11
0.0%
5
45.5%
6
54.5%
0
0
0
Parallel Persistent Infection
7
368
0.2%
28
7.6%
294
79.9%
62
16.8 %
46
12.5 %
0
Parallel - Rapid Testing Two-times IRs
32
125
0.1%
29
23.2%
92
73.6%
4
3.2 %
3
2.4 %
1
0.8 %
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
46
0.0%
0
0.0 %
45
97.8%
2
4.3 %
1
2.2 %
0
Parallel New Infection
49
5298
2.6%
100
1.9%
4716
89.0%
433
8.2 %
323
6.1 %
159
3.0 %
Parallel - Other
1
2
0.0%
1
50.0%
1
50.0%
0
0
0
Parallel Persistent Infection
1
5
0.0%
0
0.0 %
2
40.0%
3
60.0 %
3
60.0 %
0
Parallel - Rapid Testing Two-times IRs
4
15
0.0%
2
13.3%
13
86.7%
0
0
0
Wales
940
45113
21.8%
1950
4.3%
39316
87.2%
3725
8.3 %
2711
6.0 %
1136
2.5 %
Scotland - Scotland
Extended Flexible IFN-GAMMA test
1
10
0.0%
1
10.0%
8
80.0%
1
10.0 %
1
10.0 %
0
Parallel New Infection
29
2185
1.1%
141
6.5%
1906
87.2%
199
9.1 %
138
6.3 %
0
Parallel - Other
1
46
0.0%
39
84.8%
7
15.2%
1
2.2 %
0
0
Scotland
31
2241
1.1%
181
8.1%
1921
85.7%
201
9.0 %
139
6.2 %
0
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2017         | November |
| December     | 114      |
| 2018         | January  |
| February     | 206      |
| March        | 189      |
| April        | 195      |
| May          | 250      |
| June         | 187      |
| July         | 207      |
| August       | 228      |
| September    | 216      |
| October      | 274      |
| Totals (last |          |
| 12 months)   |          |
| 2430         | 227506   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1303
175903
911
8828
478
0
0
0
0
752
13
5
29
2
6941
5
38
1
2
Scotland
37
3082
25
352
47
0
0
0
0
33
4
0
0
8
188
0
37
1
27
Wales
1090
48521
541
2298
111
0
0
0
0
97
8
2
19
3
1385
67
360
3
10
Totals
2430
227506
1477
11478
636
0
0
0
0
882
25
7
48
13
8514
72
435
5
39

*Includes all animals with IFNg test negative, resample and reject outcomes

County
%Resampled %Retested
| Aberdeenshire   | 6.67 %   | 6.67 %   |
|-----------------|----------|----------|
| Avon            | 50.00 %  | 50.00 %  |
| Berks           | 1.96 %   | 1.96 %   |
| Bucks           | 8.33 %   | 12.50 %  |
| Carms           | 4.05 %   | 5.86 %   |
| Ceredigion      | 6.49 %   | 10.39 %  |
| Cheshire        | 8.38 %   | 11.98 %  |
| Corn & Scilly   | 6.53 %   | 8.73 %   |
| Cumbria         | 5.38 %   | 8.80 %   |
| Derbyshire      | 5.56 %   | 9.92 %   |
| Devon           | 3.61 %   | 6.56 %   |
| Dorset          | 5.55 %   | 7.27 %   |
| E Sussex        | 5.95 %   | 8.33 %   |
| Gloucs          | 3.97 %   | 8.22 %   |
| Gwent           | 4.17 %   | 19.91 %  |
| Hampshire       |          |          |
| Heref           | 7.11 %   | 8.63 %   |
| Kent            | 2.62 %   | 5.70 %   |
| Leics & Rut     | 2.40 %   | 5.82 %   |
| NE Wales        | 3.11 %   | 6.22 %   |
| Northants       | 4.17 %   | 8.33 %   |
| Notts           | 10.17 %  | 11.86 %  |
| Oxon            | 5.73 %   | 8.82 %   |
| Pembs           | 3.07 %   | 4.07 %   |
| Powys       | 3.98 %   | 6.39 %   |
|-------------|----------|----------|
| Shropshire  | 3.31 %   | 4.56 %   |
| Somerset    | 3.62 %   | 4.71 %   |
| Warks       | 2.19 %   | 3.95 %   |
| W Glamorgan |          |          |
| Wiltshire   | 2.80 %   | 4.56 %   |
| W Sussex    | 27.78 %  | 33.33 %  |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
19127
1054
5.5%
19262
1054
5.5%
Wales
4633
362
7.8%
4699
363
7.7%
Scotland
14
0
0.0 %
15
0.0 %
GB
23774
1416
6.0%
23976
1417
5.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
12152
844
6.9%
12264
844
6.9%
Wales
2265
120
5.3%
2301
120
5.2%
Scotland
189
7
3.7%
189
7
3.7%
GB
14606
971
6.6%
14754
971
6.6%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
140798
7718
5.5%
160008
7718
4.8%
Wales
39469
1949
4.9%
45113
1950
4.3%
Scotland
2080
181
8.7%
2241
181
8.1%
GB
182330
9848
5.4%
207362
9849
4.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
65172
3984
6.1%
82634
3984
4.8%
Wales
29318
1966
6.7%
32252
1966
6.1%
Scotland
2368
57
2.4%
2426
57
2.3%
GB
96855
6007
6.2%
117312
6007
5.1%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Berkshire
PRV-SP
1
2
0
Cheshire
PRV-SP
1
1
1
100.00 %
Cornwall and Isles of Scilly
PRV-SP
1
1
0
Devon
PRV-SP
1
2
0
Dorset
PRV-SE
1
2
0
PRV-SP
1
1
0
Hampshire
PRV-SE
1
1
0
Total
7
10
1
10.00 %
Isle of Man
SER-IOM
2
4
1
25.00 %
* Test type
Num Submissions
Berkshire
PRV-SP
1
2
0
Buckinghamshire
PRV-SE
1
1
0
Cheshire
PRV-SP
2
2
1
50.00 %
Cornwall and Isles of Scilly
PRV-SE
1
3
0
PRV-SP
1
1
0
Devon
PRV-SE
1
2
1
50.00 %
PRV-SP
1
2
0
Dorset
PRV-SE
4
25
6
24.00 %
PRV-SP
1
1
0
East Sussex
PRV-SE
1
1
0
PRV-SP
1
1
0
Hampshire
PRV-SE
2
6
1
16.67 %
Northern Somerset & South Glou
PRV-SE
2
46
8
17.39 %
Oxfordshire
PRV-SE
1
38
4
10.53 %
Somerset excl North
PRV-SE
3
46
3
6.52 %
PRV-SP
1
1
0
Suffolk
PRV-SP
2
11
0
Total
26
189
24
12.70 %
Isle of Man
SER-IOM
16
49
1
2.04 %

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

Private
% Positive
% Positive
Num Samples
Num Positives Private
|        | Test        |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|--------|-------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077 | South Wales |        1 |                65 | 15                   | 23.1 %                            |
| TC0077 | Sum:        |       65 |                15 | 23.1 %               |                                   |
|                 | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|-----------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077          | Argyll and Bute | 1        |                 3 | 3                    | 100.0 %                           |
| Carmarthenshire | 2               | 170      |                 4 | 2.4 %                |                                   |
| Ceredigion      | 1               | 85       |                 4 | 4.7 %                |                                   |
| Pembrokeshire   | 3               | 201      |                10 | 5.0 %                |                                   |
| Powys           | 1               | 345      |                 3 | 0.9 %                |                                   |
| South Wales     | 5               | 199      |                17 | 8.5 %                |                                   |
| TC0077          | Sum:            | 1,003    |                41 | 4.1 %                |                                   |