# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 164

# Report Period 1St - 30Th April 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| April 2020              |                          |          |
| Submissions             | 184                      | 1112     |
| Samples                 | Total (%)                | 10928    |
| England                 | 7482                     | 68.47 %  |
| Wales                   | 2984                     | 27.31 %  |
| Scotland                | 462                      | 4.23 %   |
| Parallel Tests          | PAR-CUL-N                | 1461     |
| PAR-CUL-P               | 917                      | 8.39 %   |
| PAR-HERD-S              | 28                       | 0.26 %   |
| PAR-NEW-IN              | 7338                     | 67.15 %  |
| PAR-OTHER               | 133                      | 1.22 %   |
| PAR-PERSIS              | 898                      | 8.22 %   |
| PAR-RAPID               | 142                      | 1.30 %   |
| Total(% of all samples) | 10917                    | 99.90 %  |
| Serial/Extended Tests   | SER-FLEXI                | 11       |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 11                       | 0.10 %   |
| Total                   | 10928                    | 100.00 % |
| Retests                 | Total (% of all samples) | 687      |
| England                 | 482                      | 4.41 %   |
| Wales                   | 187                      | 1.71 %   |
| Scotland                | 18                       | 0.16 %   |
| Resamples               | Total (% of all samples) | 535      |
| England                 | 379                      | 3.47 %   |
| Wales                   | 142                      | 1.30 %   |
| Scotland                | 14                       | 0.13 %   |
| Rejects                 | Total (% of all samples) | 3        |
| England                 | 0                        | 0.00 %   |
| Wales                   | 3                        | 0.03 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   1461 | 13.4 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    917 | 8.4 %    |
| PAR-HERD-S  |     28 | 0.3 %    |
| PAR-NEW-IN  |   7338 | 67.1 %   |
| PAR-OTHER   |    133 | 1.2 %    |
| PAR-PERSIS  |    898 | 8.2 %    |
| PAR-RAPID   |    142 | 1.3 %    |
| SER-FLEXI   |     11 | 0.1 %    |
| Total       |  10928 | 100.00 % |
Breakdown
of Samples
submitted
by County

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For April 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 2              |
| PAR-PERSIS      | 1              |
| Devon           | PAR-CUL-N      |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 1              |
| Gloucs          | PAR-CUL-N      |
| Shropshire      | PAR-CUL-N      |
| Somerset excl N | PAR-CUL-N      |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Gr Manchester  |
| N Yorks         | PAR-NEW-IN     |
| England         |                |
| 67              | 7482           |
| Wales - High TB | Carms          |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 9              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Ceredigion              | PAR-OTHER      |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 6              |
| Gwent                   | PAR-RAPID      |
| Pembs                   | PAR-OTHER      |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 16             |
| Powys                   | PAR-HERD-S     |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 6              |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-RAPID      |
| Wales - Intermediate TB | Carms          |
| NE Wales                | PAR-NEW-IN     |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 2              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | PAR-RAPID      |
| Wales - Low TB          | Gwynedd        |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 1              |
| Wales                   |                |
| 112                     | 2984           |
| Scotland - Scotland     | Argyll & Bute  |
| Kirkcudbright           | PAR-NEW-IN     |
| Wigtown                 | PAR-NEW-IN     |
| Scotland                |                |
| 5                       | 462            |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| Grand Total | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 6              |
| PAR-PERSIS      | 17             |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 7              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 6              |
| PAR-NEW-IN      | 2              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 5              |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-CUL-N      |
| PAR-PERSIS      | 7              |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 2              |
| Staffs          | PAR-CUL-N      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-PERSIS      | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| SER-FLEXI       | 2              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 68             |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 8              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Gr Manchester   | PAR-NEW-IN     |
| Humberside      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| Surrey          | PAR-NEW-IN     |
| England         |                |
| 615             | 73317          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 14             |
| PAR-PERSIS      | 24             |
| PAR-RAPID       | 28             |
| SER-FLEXI       | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 8              |
| PAR-PERSIS              | 16             |
| PAR-RAPID               | 13             |
| Gwent                   | PAR-HERD-S     |
| PAR-PERSIS              | 13             |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 3              |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 36             |
| PAR-PERSIS              | 54             |
| PAR-RAPID               | 39             |
| SER-FLEXI               | 3              |
| Powys                   | PAR-HERD-S     |
| PAR-OTHER               | 9              |
| PAR-PERSIS              | 28             |
| PAR-RAPID               | 13             |
| SER-FLEXI               | 10             |
| S Wales                 | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| PAR-PERSIS              | 2              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 4              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Ceredigion          | PAR-PERSIS     |
| PAR-RAPID           | 1              |
| SER-FLEXI           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 10             |
| PAR-RAPID           | 7              |
| SER-FLEXI           | 7              |
| Powys               | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| S Glamorgan         | PAR-RAPID      |
| SER-FLEXI           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 2              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 1              |
| Wales               |                |
| 471                 | 17590          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Berwickshire        | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 26                  | 2662           |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| Grand Total | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol April 2020

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 67           |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 5            |
| Wales           | Parallel     |
| Serial/Extended | 3            |
| Total           | 112          |
| GB              | Parallel     |
| Serial/Extended | 3            |
| Total           | 184          |
| 67.00           |              |
| 112.00          |              |
| 5.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 13           |
| Total           | 615          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 26           |
| Wales           | Parallel     |
| Serial/Extended | 32           |
| Total           | 471          |
| GB              | Parallel     |
| Serial/Extended | 45           |
| Total           | 1112         |
| 615.00          |              |
| 471.00          |              |
| 26.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
3
11
0.1%
1
9.1%
9
81.8%
2
18.2 %
1
9.1 %
0
Parallel - Herd or Group Slaughter
3
28
0.3%
2
7.1%
25
89.3%
1
3.6 %
1
3.6 %
0
Parallel New Infection
69
7338
67.1%
200
2.7%
6786
92.5%
458
6.2 %
349
4.8 %
3
0.0 %
Parallel - new infection cull area
24
1461
13.4%
64
4.4%
1328
90.9%
85
5.8 %
69
4.7 %
0
Parallel - Other
22
133
1.2%
23
17.3%
103
77.4%
8
6.0 %
7
5.3 %
0
Parallel Persistent Infection
16
898
8.2%
58
6.5%
772
86.0%
82
9.1 %
68
7.6 %
0
Parallel - persistent infection cull area
3
917
8.4%
32
3.5%
860
93.8%
35
3.8 %
25
2.7 %
0
Parallel - Rapid Testing Two-times IRs
44
142
1.3%
28
19.7%
99
69.7%
16
11.3 %
15
10.6 %
0
Total
184
10928
100.0%
408
3.7%
9982
91.3%
687
6.3 %
535
4.9 %
3
0.0 %
England - HRA
Parallel - new infection cull area
24
1461
13.4%
64
4.4%
1328
90.9%
85
5.8 %
69
4.7 %
0
Parallel Persistent Infection
2
616
5.6%
42
6.8%
523
84.9%
64
10.4 %
51
8.3 %
0
Parallel - persistent infection cull area
3
917
8.4%
32
3.5%
860
93.8%
35
3.8 %
25
2.7 %
0
England - HRA
29
2994
27.4%
138
4.6%
2711
90.5%
184
6.1 %
145
4.8 %
0
England - Edge
Parallel New Infection
33
3946
36.1%
111
2.8%
3630
92.0%
260
6.6 %
205
5.2 %
0
England - Edge
33
3946
36.1%
111
2.8%
3630
92.0%
260
6.6 %
205
5.2 %
0
England - LRA
Parallel New Infection
5
542
5.0%
5
0.9%
508
93.7%
38
7.0 %
29
5.4 %
0
England - LRA
5
542
5.0%
5
0.9%
508
93.7%
38
7.0 %
29
5.4 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
1
1
0.0%
0
0.0 %
0
0.0 %
1
100.0 %
1
100.0 %
0
Parallel - Herd or Group Slaughter
3
28
0.3%
2
7.1%
25
89.3%
1
3.6 %
1
3.6 %
0
Parallel - Other
18
111
1.0%
20
18.0%
85
76.6%
7
6.3 %
6
5.4 %
0
Parallel Persistent Infection
14
282
2.6%
16
5.7%
249
88.3%
18
6.4 %
17
6.0 %
0
Parallel - Rapid Testing Two-times IRs
39
126
1.2%
27
21.4%
87
69.0%
13
10.3 %
12
9.5 %
0
Wales - High TB
75
548
5.0%
65
11.9%
446
81.4%
40
7.3 %
37
6.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
2
10
0.1%
1
10.0%
9
90.0%
1
10.0 %
0
0
Parallel New Infection
13
1085
9.9%
60
5.5%
965
88.9%
74
6.8 %
59
5.4 %
1
0.1 %
Parallel - Other
3
17
0.2%
3
17.6%
13
76.5%
1
5.9 %
1
5.9 %
0
Parallel - Rapid Testing Two-times IRs
3
4
0.0%
1
25.0%
3
75.0%
0
0
0
Wales - Intermediate TB
21
1116
10.2%
65
5.8%
990
88.7%
76
6.8 %
60
5.4 %
1
0.1 %
Wales - Low TB
Parallel New Infection
13
1303
11.9%
15
1.2%
1244
95.5%
68
5.2 %
42
3.2 %
2
0.2 %
Parallel - Other
1
5
0.0%
0
0.0 %
5
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
2
12
0.1%
0
0.0 %
9
75.0%
3
25.0 %
3
25.0 %
0
Wales - Low TB
16
1320
12.1%
15
1.1%
1258
95.3%
71
5.4 %
45
3.4 %
2
0.2 %
Scotland - Scotland
Parallel New Infection
5
462
4.2%
9
1.9%
439
95.0%
18
3.9 %
14
3.0 %
0
Scotland - Scotland
5
462
4.2%
9
1.9%
439
95.0%
18
3.9 %
14
3.0 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
45
1868
2.0%
129
6.9%
1673
89.6%
104
5.6 %
64
3.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
21
307
0.3%
29
9.4%
268
87.3%
21
6.8 %
10
3.3 %
0
Parallel New Infection
376
42815
45.8%
1197
2.8%
38177
89.2%
2693
6.3 %
2167
5.1 %
1274
3.0 %
Parallel - new infection cull area
265
29044
31.0%
1180
4.1%
26014
89.6%
1784
6.1 %
1391
4.8 %
459
1.6 %
Parallel - Other
76
1755
1.9%
152
8.7%
1471
83.8%
153
8.7 %
132
7.5 %
0
Parallel Persistent Infection
186
12507
13.4%
757
6.1%
11042
88.3%
871
7.0 %
686
5.5 %
22
0.2 %
Parallel - persistent infection cull area
24
4832
5.2%
291
6.0%
4339
89.8%
274
5.7 %
201
4.2 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
119
441
0.5%
93
21.1%
321
72.8%
30
6.8 %
27
6.1 %
0
Total
1112
93569
100.0%
3828
4.1%
83305
89.0%
5930
6.3 %
4678
5.0 %
1758
1.9 %
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.3%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
5
415
0.4%
15
3.6%
363
87.5%
49
11.8 %
36
8.7 %
1
0.2 %
Parallel - new infection cull area
261
28630
30.6%
1164
4.1%
25645
89.6%
1735
6.1 %
1364
4.8 %
457
1.6 %
Parallel Persistent Infection
32
5574
6.0%
283
5.1%
5025
90.2%
348
6.2 %
263
4.7 %
3
0.1 %
Parallel - persistent infection cull area
24
4832
5.2%
291
6.0%
4339
89.8%
274
5.7 %
201
4.2 %
1
0.0 %
England - Edge
Parallel New Infection
251
30443
32.5%
890
2.9%
27138
89.1%
1899
6.2 %
1525
5.0 %
890
2.9 %
Parallel - new infection cull area
4
414
0.4%
16
3.9%
369
89.1%
49
11.8 %
27
6.5 %
2
0.5 %
England - LRA
Parallel New Infection
25
1785
1.9%
40
2.2%
1364
76.4%
82
4.6 %
67
3.8 %
314
17.6 %
England
615
73317
78.4%
2769
3.8%
65356
89.1%
4495
6.1 %
3524
4.8 %
1668
2.3 %
Wales - High TB
Extended Flexible IFN-GAMMA test
20
454
0.5%
42
9.3%
398
87.7%
31
6.8 %
14
3.1 %
0
Parallel - Herd or Group Slaughter
18
277
0.3%
24
8.7%
244
88.1%
18
6.5 %
9
3.2 %
0
Parallel New Infection
1
8
0.0%
2
25.0%
6
75.0%
0
0
0
Parallel - Other
68
1704
1.8%
147
8.6%
1429
83.9%
147
8.6 %
128
7.5 %
0
Parallel Persistent Infection
138
6211
6.6%
386
6.2%
5415
87.2%
484
7.8 %
391
6.3 %
19
0.3 %
Parallel - Rapid Testing Two-times IRs
104
397
0.4%
86
21.7%
289
72.8%
25
6.3 %
22
5.5 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
12
190
0.2%
17
8.9%
162
85.3%
14
7.4 %
9
4.7 %
2
1.1 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel New Infection
43
4849
5.2%
143
2.9%
4402
90.8%
351
7.2 %
303
6.2 %
1
0.0 %
Parallel - Other
6
45
0.0%
5
11.1%
37
82.2%
5
11.1 %
3
6.7 %
0
Parallel Persistent Infection
15
682
0.7%
88
12.9%
570
83.6%
29
4.3 %
24
3.5 %
0
Parallel - Rapid Testing Two-times IRs
12
31
0.0%
7
22.6%
22
71.0%
2
6.5 %
2
6.5 %
0
Wales - Low TB
Parallel New Infection
25
2653
2.8%
32
1.2%
2508
94.5%
165
6.2 %
110
4.1 %
3
0.1 %
Parallel - Other
2
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
3
13
0.0%
0
0.0 %
10
76.9%
3
23.1 %
3
23.1 %
0
Wales
471
17590
18.8%
984
5.6%
15553
88.4%
1288
7.3 %
1028
5.8 %
25
0.1 %
Scotland - Scotland
Parallel New Infection
26
2662
2.8%
75
2.8%
2396
90.0%
147
5.5 %
126
4.7 %
65
2.4 %
Scotland
26
2662
2.8%
75
2.8%
2396
90.0%
147
5.5 %
126
4.7 %
65
2.4 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | May     |
| June         | 287     |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| 2020         | January |
| February     | 323     |
| March        | 262     |
| April        | 184     |
| Totals (last |         |
| 12 months)   |         |
| 3390         | 310034  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1951
245696
1194
8276
158
0
0
0
0
1049
8
2
43
2
6935
4
54
0
1
Scotland
45
4989
25
161
4
0
0
0
0
24
0
0
4
0
111
15
2
0
0
Wales
1394
59349
700
2834
89
0
0
0
0
116
8
5
21
5
1993
47
540
0
6
Totals
3390
310034
1919
11271
251
0
0
0
0
1189
16
7
68
7
9039
66
596
0
7

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Argyll & Bute | 37.50 %  | 37.50 %                |
| Berks         | 6.77 %   | 8.83 %                 |
| Bucks         | 8.33 %   | 12.50 %                |
| Carms         | 5.52 %   | 6.63 %                 |
| Ceredigion    | 16.13 %  | 16.13 %                |
| Cheshire      | 5.01 %   | 6.22 %                 |
| Corn & Scilly | 2.39 %   | 3.53 %                 |
| Derbyshire    | 3.83 %   | 4.14 %                 |
| Devon         | 4.95 %   | 6.07 %                 |
| Dorset        | 7.15 %   | 9.09 %                 |
| E Sussex      | 5.06 %   | 5.52 %                 |
| Gloucs        | 4.95 %   | 5.45 %                 |
| Gr Manchester |          |                        |
| Gwent         |          |                        |
| Gwynedd       | 3.48 %   | 5.54 %                 |
| Kirkcudbright | 2.35 %   | 3.53 %                 |
| Leics & Rut   | 3.67 %   | 8.72 %                 |
| NE Wales      | 4.80 %   | 6.58 %                 |
| Northants     | 2.78 %   | 5.56 %                 |
| N Yorks       | 5.37 %   | 7.04 %                 |
| Oxon          | 8.33 %   | 10.42 %                |
| Pembs         | 6.87 %   | 7.22 %                 |
| Powys         | 4.20 %   | 4.20 %                 |
S Glamorgan Shropshire Somerset excl N
5.00 %
5.00 %
S Wales Warks
6.92 %
8.85 %
Wigtown
1.66 %
2.49 %
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
7439
254
3.4%
7482
254
3.4%
Wales
2923
145
5.0%
2984
145
4.9%
Scotland
461
9
2.0%
462
9
1.9%
GB
10823
408
3.8%
10928
408
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23451
918
3.9%
23776
918
3.9%
Wales
6535
217
3.3%
6544
217
3.3%
GB
29986
1135
3.8%
30320
1135
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
69064
2769
4.0%
73317
2769
3.8%
Wales
16751
983
5.9%
17590
984
5.6%
Scotland
2517
75
3.0%
2662
75
2.8%
GB
88332
3827
4.3%
93569
3828
4.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
86743
4519
5.2%
91395
4519
4.9%
Wales
22260
1348
6.1%
23740
1348
5.7%
Scotland
366
4
1.1%
366
4
1.1%
GB
109369
5871
5.4%
115501
5871
5.1%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
| % Positive      | * Test type   |
|-----------------|---------------|
| Num             |               |
| Submissions     |               |
| Num             |               |
| Samples         |               |
| Num             |               |
| Positives       |               |
| Private         |               |
| Cheshire        | PRV-SP        |
| Devon           | PRV-SE        |
| PRV-SP          | 1             |
| Dorset          | PRV-SE        |
| PRV-SP          | 1             |
| Gloucestershire | PRV-SE        |
| Oxfordshire     | PRV-SP        |
| Staffordshire   | PRV-SE        |
| Worcestershire  | PRV-SP        |
| Total           |               |
| 9               |               |
| 195             |               |
| 3               | 1.54 %        |
| Isle of Man     | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        4 |                29 | 5                    | 17.2%                             |
| Ceredigion       | 3               |       19 |                 3 | 15.8%                |                                   |
| North East Wales | 2               |       10 |                 1 | 10.0%                |                                   |
| Pembrokeshire    | 12              |       86 |                22 | 25.6%                |                                   |
| Powys            | 2               |       20 |                 2 | 10.0%                |                                   |
| South Wales      | 2               |        7 |                 4 | 57.1%                |                                   |
| TC0077           | 25              |      171 |                37 | 21.6 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 12       |               245 | 37                   | 15.1%                             |
| Ceredigion       | 7               | 26       |                 4 | 15.4%                |                                   |
| Devon            | 4               | 1,032    |                95 | 9.2%                 |                                   |
| North East Wales | 14              | 515      |                55 | 10.7%                |                                   |
| Pembrokeshire    | 28              | 443      |                78 | 17.6%                |                                   |
| Powys            | 4               | 103      |                13 | 12.6%                |                                   |
| South Wales      | 10              | 149      |                34 | 22.8%                |                                   |
| TC0077           | 79              | 2,513    |               316 | 12.6 %               |                                   |