# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 166

# Report Period 1St - 30Th June 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| June 2020               |                          |          |
| Submissions             | 244                      | 1583     |
| Samples                 | Total (%)                | 23217    |
| England                 | 18093                    | 77.93 %  |
| Wales                   | 4168                     | 17.95 %  |
| Scotland                | 956                      | 4.12 %   |
| Parallel Tests          | PAR-CUL-N                | 6636     |
| PAR-CUL-P               | 0                        | 0.00 %   |
| PAR-HERD-S              | 391                      | 1.68 %   |
| PAR-NEW-IN              | 12955                    | 55.80 %  |
| PAR-OTHER               | 590                      | 2.54 %   |
| PAR-PERSIS              | 2209                     | 9.51 %   |
| PAR-RAPID               | 80                       | 0.34 %   |
| Total(% of all samples) | 22861                    | 98.47 %  |
| Serial/Extended Tests   | SER-FLEXI                | 353      |
| SER-FRAUD               | 3                        | 0.01 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 356                      | 1.53 %   |
| Total                   | 23217                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1174     |
| England                 | 925                      | 3.98 %   |
| Wales                   | 186                      | 0.80 %   |
| Scotland                | 63                       | 0.27 %   |
| Resamples               | Total (% of all samples) | 847      |
| England                 | 669                      | 2.88 %   |
| Wales                   | 135                      | 0.58 %   |
| Scotland                | 43                       | 0.19 %   |
| Rejects                 | Total (% of all samples) | 118      |
| England                 | 114                      | 0.49 %   |
| Wales                   | 4                        | 0.02 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   6636 | 28.6 %   |
|-------------|--------|----------|
| PAR-HERD-S  |    391 | 1.7 %    |
| PAR-NEW-IN  |  12955 | 55.8 %   |
| PAR-OTHER   |    590 | 2.5 %    |
| PAR-PERSIS  |   2209 | 9.5 %    |
| PAR-RAPID   |     80 | 0.3 %    |
| SER-FLEXI   |    353 | 1.5 %    |
| SER-FRAUD   |      3 | 0.0 %    |
| Total       |  23217 | 100.00 % |
Breakdown
of Samples
submitted
by County

## Table 2A.  Summary By County For June 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| Devon           | PAR-CUL-N      |
| SER-FLEXI       | 2              |
| Dorset          | PAR-CUL-N      |
| Gloucs          | PAR-CUL-N      |
| Heref           | PAR-CUL-N      |
| Somerset excl N | PAR-CUL-N      |
| Staffs          | PAR-NEW-IN     |
| Wiltshire       | PAR-CUL-N      |
| England - Edge  | Bucks          |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England - LRA   | Lincs          |
| PAR-PERSIS      | 1              |
| Northumb        | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| England         |                |
| 132             | 18093          |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Wales - High TB         | Carms          |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 8              |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 2              |
| Ceredigion              | PAR-OTHER      |
| PAR-RAPID               | 2              |
| Gwent                   | PAR-PERSIS     |
| Pembs                   | PAR-HERD-S     |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 11             |
| PAR-PERSIS              | 8              |
| PAR-RAPID               | 11             |
| SER-FLEXI               | 5              |
| Powys                   | PAR-PERSIS     |
| PAR-RAPID               | 4              |
| W Glamorgan             | PAR-RAPID      |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 3              |
| Powys                   | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| Wales               |                |
| 103                 | 4168           |
| Scotland - Scotland | Argyll & Bute  |
| Kirkcudbright       | PAR-NEW-IN     |
| Scotland            |                |
| 9                   | 956            |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 7              |
| PAR-PERSIS      | 17             |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 8              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 5              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 7              |
| PAR-NEW-IN      | 2              |
| SER-FLEXI       | 5              |
| Gloucs          | PAR-CUL-N      |
| PAR-PERSIS      | 2              |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-CUL-N      |
| PAR-PERSIS      | 7              |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 3              |
| Staffs          | PAR-CUL-N      |
| PAR-NEW-IN      | 24             |
| Wiltshire       | PAR-CUL-N      |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| PAR-CUL-P      | 4              |
| PAR-PERSIS     | 2              |
| SER-FLEXI      | 2              |
| England - Edge | Berks          |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-CUL-N      |
| PAR-NEW-IN     | 94             |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| Hampshire      | PAR-CUL-N      |
| PAR-NEW-IN     | 13             |
| Leics & Rut    | PAR-NEW-IN     |
| Northants      | PAR-NEW-IN     |
| Notts          | PAR-NEW-IN     |
| Oxon           | PAR-NEW-IN     |
| Warks          | PAR-NEW-IN     |
| PAR-PERSIS     | 2              |
| England - LRA  | Cleveland      |
| Cumbria        | PAR-NEW-IN     |
| Gr Manchester  | PAR-NEW-IN     |
| Humberside     | PAR-NEW-IN     |
| Lincs          | PAR-NEW-IN     |
| PAR-PERSIS     | 1              |
| Norfolk        | PAR-NEW-IN     |
| Northumb       | PAR-NEW-IN     |
| N Yorks        | PAR-NEW-IN     |
| Surrey         | PAR-NEW-IN     |
| W Yorks        | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England         |                |
| 862             | 104673         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 24             |
| PAR-PERSIS      | 38             |
| PAR-RAPID       | 35             |
| SER-FLEXI       | 6              |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 16             |
| PAR-PERSIS      | 16             |
| PAR-RAPID       | 17             |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 16             |
| PAR-RAPID       | 10             |
| SER-FLEXI       | 3              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 54             |
| PAR-PERSIS      | 72             |
| PAR-RAPID       | 65             |
| SER-FLEXI       | 9              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 9              |
| PAR-PERSIS      | 41             |
| PAR-RAPID       | 22             |
| SER-FLEXI       | 10             |
| S Wales         | PAR-PERSIS     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-OTHER      |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 7              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 4              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 11             |
| PAR-RAPID               | 11             |
| SER-FLEXI               | 8              |
| Powys                   | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 2              |
| S Glamorgan             | PAR-RAPID      |
| SER-FLEXI               | 3              |
| Wales - Low TB          | Gwynedd        |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 2              |
| NE Wales                | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-OTHER           | 1              |
| PAR-PERSIS          | 1              |
| PAR-RAPID           | 1              |
| Wales               |                |
| 678                 | 25583          |
| Scotland - Scotland | Argyll & Bute  |
| PAR-OTHER           | 3              |
| Ayrshire            | PAR-NEW-IN     |
| Berwickshire        | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| Perthshire          | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 43                  | 4101           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol June 2020

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 3            |
| Total           | 132          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 9            |
| Wales           | Parallel     |
| Serial/Extended | 7            |
| Total           | 103          |
| GB              | Parallel     |
| Serial/Extended | 10           |
| Total           | 244          |
| 132.00          |              |
| 103.00          |              |
| 9.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 16           |
| Total           | 862 104673   |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 43           |
| Wales           | Parallel     |
| Serial/Extended | 43           |
| Total           | 678          |
| GB              | Parallel     |
| Serial/Extended | 59           |
| Total           | 1583 134357  |
| 862.00          |              |
| 678.00          |              |
| 43.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (June 2020)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
9
353
1.5%
16
4.5%
321
90.9%
16
4.5 %
10
2.8 %
6
1.7 %
Parallel - Herd or Group Slaughter
2
391
1.7%
13
3.3%
372
95.1%
11
2.8 %
6
1.5 %
0
Parallel New Infection
105
12955
55.8%
407
3.1%
12039
92.9%
668
5.2 %
504
3.9 %
5
0.0 %
Parallel - new infection cull area
44
6636
28.6%
248
3.7%
6078
91.6%
319
4.8 %
207
3.1 %
103
1.6 %
Parallel - Other
26
590
2.5%
69
11.7%
484
82.0%
55
9.3 %
37
6.3 %
0
Parallel Persistent Infection
29
2209
9.5%
88
4.0%
2035
92.1%
100
4.5 %
82
3.7 %
4
0.2 %
Parallel - Rapid Testing Two-times IRs
28
80
0.3%
19
23.8%
60
75.0%
5
6.3 %
1
1.3 %
0
Serial - Anomalous Reaction
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Total
244
23217
100.0%
860
3.7%
21392
92.1%
1174
5.1 %
847
3.6 %
118
0.5 %
England - HRA
Extended Flexible IFN-GAMMA test
2
251
1.1%
5
2.0%
234
93.2%
9
3.6 %
6
2.4 %
6
2.4 %
Parallel New Infection
12
2161
9.3%
61
2.8%
2021
93.5%
106
4.9 %
79
3.7 %
0
Parallel - new infection cull area
44
6636
28.6%
248
3.7%
6078
91.6%
319
4.8 %
207
3.1 %
103
1.6 %
Serial - Anomalous Reaction
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
England - HRA
59
9051
39.0%
314
3.5%
8336
92.1%
434
4.8 %
292
3.2 %
109
1.2 %
England - Edge
Parallel New Infection
66
8182
35.2%
253
3.1%
7613
93.0%
426
5.2 %
311
3.8 %
5
0.1 %
Parallel Persistent Infection
2
479
2.1%
20
4.2%
446
93.1%
20
4.2 %
13
2.7 %
0
England - Edge
68
8661
37.3%
273
3.2%
8059
93.0%
446
5.1 %
324
3.7 %
5
0.1 %
England - LRA
Parallel New Infection
4
349
1.5%
25
7.2%
273
78.2%
42
12.0 %
51
14.6 %
0
Parallel Persistent Infection
1
32
0.1%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
England - LRA
5
381
1.6%
25
6.6%
303
79.5%
45
11.8 %
53
13.9 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
102
0.4%
11
10.8%
87
85.3%
7
6.9 %
4
3.9 %
0
Parallel - Herd or Group Slaughter
2
391
1.7%
13
3.3%
372
95.1%
11
2.8 %
6
1.5 %
0
Parallel New Infection
1
254
1.1%
14
5.5%
226
89.0%
14
5.5 %
14
5.5 %
0
Parallel - Other
20
218
0.9%
56
25.7%
153
70.2%
15
6.9 %
9
4.1 %
0
Parallel Persistent Infection
24
1416
6.1%
57
4.0%
1304
92.1%
61
4.3 %
51
3.6 %
4
0.3 %
Parallel - Rapid Testing Two-times IRs
21
63
0.3%
17
27.0%
45
71.4%
5
7.9 %
1
1.6 %
0
Wales - High TB
75
2444
10.5%
168
6.9%
2187
89.5%
113
4.6 %
85
3.5 %
4
0.2 %
Wales - Intermediate TB
Parallel New Infection
12
1196
5.2%
34
2.8%
1133
94.7%
44
3.7 %
29
2.4 %
0
Parallel - Other
3
11
0.0%
2
18.2%
9
81.8%
0
0
0
Parallel Persistent Infection
2
282
1.2%
11
3.9%
255
90.4%
16
5.7 %
16
5.7 %
0
Parallel - Rapid Testing Two-times IRs
7
17
0.1%
2
11.8%
15
88.2%
0
0
0
Wales - Intermediate TB
24
1506
6.5%
49
3.3%
1412
93.8%
60
4.0 %
45
3.0 %
0
Wales - Low TB
Parallel New Infection
4
218
0.9%
6
2.8%
207
95.0%
13
6.0 %
5
2.3 %
0
Wales - Low TB
4
218
0.9%
6
2.8%
207
95.0%
13
6.0 %
5
2.3 %
0
Scotland - Scotland
Parallel New Infection
6
595
2.6%
14
2.4%
566
95.1%
23
3.9 %
15
2.5 %
0
Parallel - Other
3
361
1.6%
11
3.0%
322
89.2%
40
11.1 %
28
7.8 %
0
Scotland - Scotland
9
956
4.1%
25
2.6%
888
92.9%
63
6.6 %
43
4.5 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
58
2361
1.8%
160
6.8%
2114
89.5%
124
5.3 %
77
3.3 %
10
0.4 %
Parallel - Herd or Group Slaughter
25
773
0.6%
47
6.1%
702
90.8%
40
5.2 %
24
3.1 %
0
Parallel New Infection
572
64088
47.7%
1875
2.9%
57866
90.3%
3899
6.1 %
3068
4.8 %
1279
2.0 %
Parallel - new infection cull area
359
42031
31.3%
1798
4.3%
37821
90.0%
2451
5.8 %
1846
4.4 %
566
1.3 %
Parallel - Other
123
2461
1.8%
233
9.5%
2057
83.6%
210
8.5 %
171
6.9 %
0
Parallel Persistent Infection
243
16428
12.2%
957
5.8%
14636
89.1%
1025
6.2 %
807
4.9 %
28
0.2 %
Parallel - persistent infection cull area
27
5534
4.1%
333
6.0%
4951
89.5%
336
6.1 %
249
4.5 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
175
678
0.5%
143
21.1%
503
74.2%
44
6.5 %
32
4.7 %
0
Serial - Anomalous Reaction
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
Total
1583 134357
100.0%
5546
4.1%
120653
89.8%
8129
6.1 %
6274
4.7 %
1884
1.4 %
England - HRA
Extended Flexible IFN-GAMMA test
15
1475
1.1%
75
5.1%
1347
91.3%
68
4.6 %
47
3.2 %
6
0.4 %
Parallel New Infection
29
4460
3.3%
132
3.0%
4150
93.0%
237
5.3 %
177
4.0 %
1
0.0 %
Parallel - new infection cull area
355
41617
31.0%
1782
4.3%
37452
90.0%
2402
5.8 %
1819
4.4 %
564
1.4 %
Parallel Persistent Infection
34
5291
3.9%
256
4.8%
4801
90.7%
307
5.8 %
231
4.4 %
3
0.1 %
Parallel - persistent infection cull area
27
5534
4.1%
333
6.0%
4951
89.5%
336
6.1 %
249
4.5 %
1
0.0 %
Serial - Anomalous Reaction
1
3
0.0%
0
0.0 %
3
100.0%
0
0
0
England - Edge
Parallel New Infection
361
43074
32.1%
1309
3.0%
38805
90.1%
2649
6.1 %
2065
4.8 %
895
2.1 %
Parallel - new infection cull area
4
414
0.3%
16
3.9%
369
89.1%
49
11.8 %
27
6.5 %
2
0.5 %
Parallel Persistent Infection
2
479
0.4%
20
4.2%
446
93.1%
20
4.2 %
13
2.7 %
0
England - LRA
Parallel New Infection
33
2294
1.7%
74
3.2%
1782
77.7%
135
5.9 %
124
5.4 %
314
13.7 %
Parallel Persistent Infection
1
32
0.0%
0
0.0 %
30
93.8%
3
9.4 %
2
6.3 %
0
England
862 104673
77.9%
3997
3.8%
94136
89.9%
6206
5.9 %
4754
4.5 %
1786
1.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
30
644
0.5%
67
10.4%
554
86.0%
41
6.4 %
21
3.3 %
2
0.3 %
Parallel - Herd or Group Slaughter
22
743
0.6%
42
5.7%
678
91.3%
37
5.0 %
23
3.1 %
0
Parallel New Infection
3
298
0.2%
18
6.0%
266
89.3%
14
4.7 %
14
4.7 %
0
Parallel - Other
104
2030
1.5%
215
10.6%
1677
82.6%
163
8.0 %
138
6.8 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel Persistent Infection
186
9532
7.1%
578
6.1%
8419
88.3%
636
6.7 %
510
5.4 %
25
0.3 %
Parallel - Rapid Testing Two-times IRs
151
607
0.5%
131
21.6%
449
74.0%
39
6.4 %
27
4.4 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
13
242
0.2%
18
7.4%
213
88.0%
15
6.2 %
9
3.7 %
2
0.8 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0
Parallel New Infection
72
7095
5.3%
209
2.9%
6498
91.6%
462
6.5 %
387
5.5 %
1
0.0 %
Parallel - Other
14
64
0.0%
7
10.9%
53
82.8%
6
9.4 %
4
6.3 %
0
Parallel Persistent Infection
18
1032
0.8%
103
10.0%
886
85.9%
49
4.7 %
43
4.2 %
0
Parallel - Rapid Testing Two-times IRs
21
58
0.0%
12
20.7%
44
75.9%
2
3.4 %
2
3.4 %
0
Wales - Low TB
Parallel New Infection
35
3149
2.3%
42
1.3%
2962
94.1%
210
6.7 %
142
4.5 %
3
0.1 %
Parallel - Other
2
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
3
13
0.0%
0
0.0 %
10
76.9%
3
23.1 %
3
23.1 %
0
Wales
678
25583
19.0%
1447
5.7%
22770
89.0%
1691
6.6 %
1333
5.2 %
33
0.1 %
Scotland - Scotland
Parallel New Infection
39
3718
2.8%
91
2.4%
3403
91.5%
192
5.2 %
159
4.3 %
65
1.7 %
Parallel - Other
3
361
0.3%
11
3.0%
322
89.2%
40
11.1 %
28
7.8 %
0
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Scotland
43
4101
3.1%
102
2.5%
3747
91.4%
232
5.7 %
187
4.6 %
65
1.6 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | July    |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| 2020         | January |
| February     | 323     |
| March        | 262     |
| April        | 184     |
| May          | 227     |
| June         | 244     |
| Totals (last |         |
| 12 months)   |         |
| 3210         | 296178  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1822
231757
1145
7896
288
0
0
0
0
990
7
2
40
3
6236
0
31
0
1
Scotland
57
5923
28
133
14
0
0
0
0
12
0
0
2
0
75
0
18
0
0
Wales
1331
58498
687
2880
120
0
0
0
0
99
11
4
25
1
1948
88
490
0
9
Totals
3210
296178
1860
10909
422
0
0
0
0
1101
18
6
67
4
8259
88
539
0
10

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Argyll & Bute | 7.76 %   | 11.08 %                |
| Avon          |          |                        |
| Bucks         | 1.05 %   | 2.11 %                 |
| Carms         | 4.89 %   | 6.15 %                 |
| Ceredigion    | 3.28 %   | 4.92 %                 |
| Cheshire      | 3.59 %   | 4.69 %                 |
| Corn & Scilly | 4.25 %   | 5.70 %                 |
| Derbyshire    | 3.50 %   | 5.09 %                 |
| Devon         | 2.86 %   | 4.37 %                 |
| Dorset        | 7.50 %   | 10.00 %                |
| E Sussex      | 100.00 % | 100.00 %               |
| Gloucs        | 8.64 %   | 12.35 %                |
| Gwent         | 37.50 %  | 37.50 %                |
| Gwynedd       | 25.00 %  | 25.00 %                |
| Hampshire     | 3.72 %   | 6.34 %                 |
| Heref         | 3.03 %   | 4.65 %                 |
| Kirkcudbright | 2.52 %   | 3.87 %                 |
| Leics & Rut   | 4.98 %   | 6.03 %                 |
| Lincs         | 8.57 %   | 11.43 %                |
| NE Wales      | 2.33 %   | 3.96 %                 |
| Northants     | 3.69 %   | 4.67 %                 |
| Northumb      | 16.29 %  | 13.03 %                |
| Notts         | 11.54 %  | 11.54 %                |
| N Yorks       | 2.56 %   |                        |
| Oxon            | 3.75 %   | 5.15 %   |
|-----------------|----------|----------|
| Pembs           | 2.62 %   | 3.55 %   |
| Powys           | 7.10 %   | 7.10 %   |
| Somerset excl N | 1.95 %   | 3.90 %   |
| Staffs          | 3.66 %   | 4.91 %   |
| Warks           | 3.06 %   | 4.52 %   |
| W Glamorgan     |          |          |
| Wiltshire       | 4.23 %   | 4.76 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
17917
612
3.4%
18093
612
3.4%
Wales
4126
223
5.4%
4168
223
5.4%
Scotland
947
25
2.6%
956
25
2.6%
GB
22990
860
3.7%
23217
860
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
21688
744
3.4%
21816
744
3.4%
Wales
4402
219
5.0%
4459
219
4.9%
Scotland
12
1
8.3%
12
1
8.3%
GB
26102
964
3.7%
26287
964
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
99532
3997
4.0%
104673
3997
3.8%
Wales
24035
1446
6.0%
25583
1447
5.7%
Scotland
3609
102
2.8%
4101
102
2.5%
GB
127175
5545
4.4%
134357
5546
4.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
128595
6127
4.8%
136690
6127
4.5%
Wales
30224
1765
5.8%
32584
1765
5.4%
Scotland
859
59
6.9%
871
59
6.8%
GB
159676
7951
5.0%
170145
7951
4.7%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
| % Positive      | * Test type   |
|-----------------|---------------|
| Num             |               |
| Submissions     |               |
| Num             |               |
| Samples         |               |
| Num             |               |
| Positives       |               |
| Private         |               |
| Cheshire        | PRV-SP        |
| Devon           | PRV-SE        |
| PRV-SP          | 1             |
| Dorset          | PRV-SE        |
| PRV-SP          | 1             |
| Gloucestershire | PRV-SE        |
| Oxfordshire     | PRV-SP        |
| Staffordshire   | PRV-SE        |
| Worcestershire  | PRV-SP        |
| Total           |               |
| 9               |               |
| 195             |               |
| 3               | 1.54 %        |
| Isle of Man     | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |       10 |               183 | 42                   | 23.0%                             |
| Ceredigion       | 3               |       50 |                 2 | 4.0%                 |                                   |
| North East Wales | 2               |       18 |                 4 | 22.2%                |                                   |
| Pembrokeshire    | 15              |      244 |                42 | 17.2%                |                                   |
| Powys            | 5               |       27 |                12 | 44.4%                |                                   |
| TC0077           | 35              |      522 |               102 | 19.5 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 24       |               451 | 86                   | 19.1%                             |
| Ceredigion       | 13              | 90       |                 8 | 8.9%                 |                                   |
| Devon            | 4               | 1,032    |                95 | 9.2%                 |                                   |
| North East Wales | 20              | 548      |                61 | 11.1%                |                                   |
| Pembrokeshire    | 58              | 862      |               154 | 17.9%                |                                   |
| Powys            | 14              | 188      |                36 | 19.1%                |                                   |
| South Wales      | 10              | 149      |                34 | 22.8%                |                                   |
| Wiltshire        | 1               | 291      |                 3 | 1.0%                 |                                   |
| TC0077           | 144             | 3,611    |               477 | 13.2 %               |                                   |