# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 155

# Report Period 1St - 31St July 2019 Issued 27 August 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| July 2019               |                          |          |
| Submissions             | 316                      | 2160     |
| Samples                 | Total (%)                | 32549    |
| England                 | 25006                    | 76.83 %  |
| Wales                   | 6558                     | 20.15 %  |
| Scotland                | 985                      | 3.03 %   |
| Parallel Tests          | PAR-CUL-N                | 6170     |
| PAR-CUL-P               | 2137                     | 6.57 %   |
| PAR-HERD-S              | 33                       | 0.10 %   |
| PAR-NEW-IN              | 13685                    | 42.04 %  |
| PAR-OTHER               | 396                      | 1.22 %   |
| PAR-PERSIS              | 8966                     | 27.55 %  |
| PAR-RAPID               | 83                       | 0.26 %   |
| Total(% of all samples) | 31470                    | 96.68 %  |
| Serial/Extended Tests   | SER-FLEXI                | 1079     |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 1079                     | 3.32 %   |
| Total                   | 32549                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2283     |
| England                 | 1963                     | 6.03 %   |
| Wales                   | 270                      | 0.83 %   |
| Scotland                | 50                       | 0.15 %   |
| Resamples               | Total (% of all samples) | 1669     |
| England                 | 1444                     | 4.44 %   |
| Wales                   | 183                      | 0.56 %   |
| Scotland                | 42                       | 0.13 %   |
| Rejects                 | Total (% of all samples) | 3        |
| England                 | 3                        | 0.01 %   |
| Wales                   | 0                        | 0.00 %   |
| Scotland                | 0                        | 0.00 %   |

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   6170 | 19.0 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   2137 | 6.6 %    |
| PAR-HERD-S  |     33 | 0.1 %    |
| PAR-NEW-IN  |  13685 | 42.0 %   |
| PAR-OTHER   |    396 | 1.2 %    |
| PAR-PERSIS  |   8966 | 27.5 %   |
| PAR-RAPID   |     83 | 0.3 %    |
| SER-FLEXI   |   1079 | 3.3 %    |
| Total       |  32549 | 100.00 % |
Breakdown
of Samples
submitted
by County

Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For July 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 3              |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-PERSIS      | 6              |
| Devon           | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 1              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| SER-FLEXI       | 1              |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| SER-FLEXI       | 2              |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 4              |
| Shropshire      | PAR-PERSIS     |
| Somerset excl N | PAR-CUL-N      |
| PAR-PERSIS      | 3              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-PERSIS      | 2              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Cheshire        | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Kent            | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| England         |                |
| 194             | 25006          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 8              |
| SER-FLEXI       | 2              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 5              |
| PAR-RAPID       | 4              |
| Gwent           | PAR-PERSIS     |
| Pembs           | PAR-OTHER      |
| PAR-PERSIS      | 15             |
| PAR-RAPID       | 15             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FLEXI               | 10             |
| Powys                   | PAR-HERD-S     |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-OTHER      |
| NE Wales                | PAR-NEW-IN     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | PAR-RAPID      |
| SER-FLEXI               | 2              |
| S Wales                 | PAR-RAPID      |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 2              |
| SER-FLEXI               | 1              |
| Wales                   |                |
| 117                     | 6558           |
| Scotland - Scotland     | Argyll & Bute  |
| Dumfries & G            | PAR-NEW-IN     |
| Wigtown                 | PAR-NEW-IN     |
| Scotland                |                |
| 5                       | 985            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| PAR-OTHER     | 1              |
| PAR-PERSIS    | 9              |
| SER-FRAUD     | 2              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 4              |
| PAR-NEW-IN    | 2              |
| PAR-PERSIS    | 24             |
| SER-FLEXI     | 2              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 20             |
| PAR-NEW-IN    | 4              |
| PAR-OTHER     | 7              |
| PAR-PERSIS    | 19             |
| SER-FLEXI     | 8              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 10             |
| PAR-NEW-IN    | 2              |
| SER-FLEXI     | 4              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 6              |
| PAR-HERD-S    | 4              |
| SER-FLEXI     | 2              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 2              |
| PAR-NEW-IN    | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country            | County         |
|--------------------|----------------|
| Submission         |                |
| Reasons*           |                |
| No.                |                |
| subs               |                |
| Samples            | Gamma Positive |
| n                  | % of total     |
| PAR-OTHER          | 1              |
| PAR-PERSIS         | 23             |
| Shropshire         | PAR-CUL-P      |
| PAR-NEW-IN         | 1              |
| PAR-OTHER          | 4              |
| PAR-PERSIS         | 7              |
| SER-FLEXI          | 5              |
| Somerset excl N    | PAR-CUL-N      |
| PAR-OTHER          | 2              |
| PAR-PERSIS         | 13             |
| Staffs             | PAR-PERSIS     |
| Wiltshire          | PAR-CUL-N      |
| PAR-CUL-P          | 11             |
| PAR-NEW-IN         | 4              |
| PAR-OTHER          | 2              |
| PAR-PERSIS         | 4              |
| SER-FLEXI          | 1              |
| England - Edge     | Berks          |
| PAR-PERSIS         | 3              |
| Bucks              | PAR-NEW-IN     |
| Cheshire           | PAR-CUL-N      |
| PAR-NEW-IN         | 146            |
| PAR-PERSIS         | 5              |
| Derbyshire         | PAR-NEW-IN     |
| E Sussex           | PAR-NEW-IN     |
| Hampshire          | PAR-NEW-IN     |
| SER-NSR            | 2              |
| Leicestershire and |                |
| Rutland            |                |
| PAR-NEW-IN         | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 6              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Essex           | PAR-NEW-IN     |
| Gr Manchester   | PAR-NEW-IN     |
| Herts           | PAR-NEW-IN     |
| I of Wight      | PAR-NEW-IN     |
| Kent            | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| SER-FLEXI       | 2              |
| S Yorks         | PAR-NEW-IN     |
| W Sussex        | PAR-NEW-IN     |
| W Yorks         | PAR-HERD-S     |
| England         |                |
| 1288            | 161696         |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 11             |
| PAR-PERSIS      | 52             |
| PAR-RAPID       | 53             |
| SER-FLEXI       | 28             |
| Ceredigion      | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| PAR-NEW-IN  | 2              |
| PAR-OTHER   | 2              |
| PAR-PERSIS  | 19             |
| PAR-RAPID   | 33             |
| SER-FLEXI   | 3              |
| Gwent       | PAR-HERD-S     |
| PAR-OTHER   | 1              |
| PAR-PERSIS  | 14             |
| PAR-RAPID   | 22             |
| SER-FLEXI   | 1              |
| Pembs       | PAR-HERD-S     |
| PAR-OTHER   | 14             |
| PAR-PERSIS  | 89             |
| PAR-RAPID   | 85             |
| SER-FLEXI   | 31             |
| Powys       | PAR-HERD-S     |
| PAR-NEW-IN  | 15             |
| PAR-OTHER   | 1              |
| PAR-PERSIS  | 27             |
| PAR-RAPID   | 44             |
| SER-FLEXI   | 9              |
| S Wales     | PAR-HERD-S     |
| PAR-OTHER   | 1              |
| PAR-PERSIS  | 3              |
| PAR-RAPID   | 1              |
| SER-FLEXI   | 3              |
| W Glamorgan | PAR-HERD-S     |
| PAR-PERSIS  | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 12             |
| SER-FLEXI               | 26             |
| Powys                   | PAR-NEW-IN     |
| PAR-RAPID               | 1              |
| S Glamorgan             | PAR-RAPID      |
| SER-FLEXI               | 3              |
| S Wales                 | PAR-RAPID      |
| W Glamorgan             | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 2              |
| Wales - Low TB          | Gwynedd        |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 1              |
| SER-FLEXI           | 1              |
| Wales               |                |
| 859                 | 39142          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 13                  | 1856           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 4            |
| Total           | 194          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 5            |
| Wales           | Parallel     |
| Serial/Extended | 21           |
| Total           | 117          |
| GB              | Parallel     |
| Serial/Extended | 25           |
| Total           | 316          |
| 194.00          |              |
| 117.00          |              |
| 5.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 28           |
| Total           | 1288 161696  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 13           |
| Wales           | Parallel     |
| Serial/Extended | 108          |
| Total           | 859          |
| GB              | Parallel     |
| Serial/Extended | 136          |
| Total           | 2160 202694  |

#######
859.00
13.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (July 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
25
1079
3.3%
80
7.4%
978
90.6%
39
3.6 %
21
1.9 %
0
Parallel - Herd or Group Slaughter
5
33
0.1%
8
24.2%
22
66.7%
5
15.2 %
3
9.1 %
0
Parallel New Infection
113
13685
42.0%
362
2.6%
12476
91.2%
1165
8.5 %
862
6.3 %
1
0.0 %
Parallel - new infection cull area
60
6170
19.0%
317
5.1%
5520
89.5%
461
7.5 %
332
5.4 %
1
0.0 %
Parallel - Other
8
396
1.2%
25
6.3%
344
86.9%
31
7.8 %
27
6.8 %
0
Parallel Persistent Infection
57
8966
27.5%
286
3.2%
8381
93.5%
420
4.7 %
298
3.3 %
1
0.0 %
Parallel - persistent infection cull area
10
2137
6.6%
135
6.3%
1879
87.9%
158
7.4 %
123
5.8 %
0
Parallel - Rapid Testing Two-times IRs
38
83
0.3%
6
7.2%
74
89.2%
4
4.8 %
3
3.6 %
0
Total
316
32549
100.0%
1219
3.7%
29674
91.2%
2283
7.0 %
1669
5.1 %
3
0.0 %
England - HRA
Extended Flexible IFN-GAMMA test
4
58
0.2%
5
8.6%
52
89.7%
5
8.6 %
1
1.7 %
0
Parallel New Infection
2
104
0.3%
10
9.6%
81
77.9%
21
20.2 %
13
12.5 %
0
Parallel - new infection cull area
60
6170
19.0%
317
5.1%
5520
89.5%
461
7.5 %
332
5.4 %
1
0.0 %
Parallel - Other
1
21
0.1%
1
4.8%
13
61.9%
7
33.3 %
7
33.3 %
0
Parallel Persistent Infection
21
4490
13.8%
167
3.7%
4154
92.5%
229
5.1 %
168
3.7 %
1
0.0 %
Parallel - persistent infection cull area
10
2137
6.6%
135
6.3%
1879
87.9%
158
7.4 %
123
5.8 %
0
England - HRA
98
12980
39.9%
635
4.9%
11699
90.1%
881
6.8 %
644
5.0 %
2
0.0 %
England - Edge
Parallel New Infection
88
11050
33.9%
286
2.6%
10071
91.1%
954
8.6 %
708
6.4 %
1
0.0 %
Parallel Persistent Infection
2
299
0.9%
7
2.3%
276
92.3%
23
7.7 %
16
5.4 %
0
England - Edge
90
11349
34.9%
293
2.6%
10347
91.2%
977
8.6 %
724
6.4 %
1
0.0 %
England - LRA
Parallel New Infection
6
677
2.1%
20
3.0%
581
85.8%
105
15.5 %
76
11.2 %
0
England - LRA
6
677
2.1%
20
3.0%
581
85.8%
105
15.5 %
76
11.2 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
13
771
2.4%
52
6.7%
706
91.6%
25
3.2 %
13
1.7 %
0
Parallel - Herd or Group Slaughter
4
26
0.1%
5
19.2%
18
69.2%
5
19.2 %
3
11.5 %
0
Parallel - Other
6
371
1.1%
24
6.5%
329
88.7%
22
5.9 %
18
4.9 %
0
Parallel Persistent Infection
32
3748
11.5%
105
2.8%
3542
94.5%
151
4.0 %
101
2.7 %
0
Parallel - Rapid Testing Two-times IRs
34
66
0.2%
4
6.1%
59
89.4%
4
6.1 %
3
4.5 %
0
Wales - High TB
89
4982
15.3%
190
3.8%
4654
93.4%
207
4.2 %
138
2.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
7
163
0.5%
19
11.7%
139
85.3%
6
3.7 %
5
3.1 %
0
Parallel - Herd or Group Slaughter
1
7
0.0%
3
42.9%
4
57.1%
0
0
0
Parallel New Infection
8
676
2.1%
21
3.1%
640
94.7%
26
3.8 %
15
2.2 %
0
Parallel - Other
1
4
0.0%
0
0.0 %
2
50.0%
2
50.0 %
2
50.0 %
0
Parallel - Rapid Testing Two-times IRs
4
17
0.1%
2
11.8%
15
88.2%
0
0
0
Wales - Intermediate TB
21
867
2.7%
45
5.2%
800
92.3%
34
3.9 %
22
2.5 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
87
0.3%
4
4.6%
81
93.1%
3
3.4 %
2
2.3 %
0
Parallel New Infection
4
193
0.6%
3
1.6%
182
94.3%
9
4.7 %
8
4.1 %
0
Parallel Persistent Infection
2
429
1.3%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Wales - Low TB
7
709
2.2%
14
2.0%
672
94.8%
29
4.1 %
23
3.2 %
0
Scotland - Scotland
Parallel New Infection
5
985
3.0%
22
2.2%
921
93.5%
50
5.1 %
42
4.3 %
0
Scotland - Scotland
5
985
3.0%
22
2.2%
921
93.5%
50
5.1 %
42
4.3 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
132
4986
2.5%
339
6.8%
4365
87.5%
251
5.0 %
182
3.7 %
100
2.0 %
Parallel - Herd or Group Slaughter
109
4077
2.0%
328
8.0%
3480
85.4%
317
7.8 %
262
6.4 %
8
0.2 %
Parallel New Infection
818
95385
47.1%
3438
3.6%
85745
89.9%
6814
7.1 %
5427
5.7 %
791
0.8 %
Parallel - new infection cull area
366
42147
20.8%
2182
5.2%
37456
88.9%
2762
6.6 %
2145
5.1 %
364
0.9 %
Parallel - Other
58
3733
1.8%
248
6.6%
3085
82.6%
433
11.6 %
358
9.6 %
42
1.1 %
Parallel Persistent Infection
340
40038
19.8%
1810
4.5%
35771
89.3%
2485
6.2 %
1911
4.8 %
549
1.4 %
Parallel - persistent infection cull area
57
11493
5.7%
659
5.7%
10192
88.7%
777
6.8 %
635
5.5 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
276
809
0.4%
166
20.5%
576
71.2%
57
7.0 %
41
5.1 %
26
3.2 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
2160 202694
100.0%
9170
4.5%
180695
89.1%
13897
6.9 %
10962
5.4 %
1887
0.9 %
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.8%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
14
1282
0.6%
128
10.0%
1021
79.6%
166
12.9 %
133
10.4 %
0
Parallel - new infection cull area
365
41887
20.7%
2163
5.2%
37227
88.9%
2750
6.6 %
2133
5.1 %
364
0.9 %
Parallel - Other
17
1354
0.7%
80
5.9%
1003
74.1%
266
19.6 %
229
16.9 %
42
3.1 %
Parallel Persistent Infection
106
17367
8.6%
918
5.3%
15078
86.8%
1121
6.5 %
844
4.9 %
528
3.0 %
Parallel - persistent infection cull area
57
11493
5.7%
659
5.7%
10192
88.7%
777
6.8 %
635
5.5 %
7
0.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
619
77138
38.1%
2736
3.5%
69408
90.0%
5450
7.1 %
4325
5.6 %
685
0.9 %
Parallel - new infection cull area
1
260
0.1%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
14
1314
0.6%
27
2.1%
1234
93.9%
66
5.0 %
53
4.0 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
61
7862
3.9%
257
3.3%
7034
89.5%
660
8.4 %
533
6.8 %
38
0.5 %
England
1288 161696
79.8%
7075
4.4%
143966
89.0%
11372
7.0 %
8963
5.5 %
1709
1.1 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Wales - High TB
Extended Flexible IFN-GAMMA test
75
2411
1.2%
171
7.1%
2157
89.5%
105
4.4 %
81
3.4 %
2
0.1 %
Parallel - Herd or Group Slaughter
97
3919
1.9%
303
7.7%
3363
85.8%
299
7.6 %
246
6.3 %
8
0.2 %
Parallel New Infection
20
797
0.4%
43
5.4%
715
89.7%
41
5.1 %
39
4.9 %
0
Parallel - Other
30
1825
0.9%
117
6.4%
1609
88.2%
131
7.2 %
99
5.4 %
0
Parallel Persistent Infection
205
19581
9.7%
795
4.1%
17813
91.0%
1212
6.2 %
954
4.9 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
241
704
0.3%
150
21.3%
490
69.6%
54
7.7 %
38
5.4 %
26
3.7 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
32
832
0.4%
84
10.1%
657
79.0%
45
5.4 %
38
4.6 %
53
6.4 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
70
5771
2.8%
177
3.1%
5240
90.8%
378
6.5 %
302
5.2 %
52
0.9 %
Parallel - Other
11
554
0.3%
51
9.2%
473
85.4%
36
6.5 %
30
5.4 %
0
Parallel Persistent Infection
13
1347
0.7%
63
4.7%
1237
91.8%
69
5.1 %
47
3.5 %
0
Parallel - Rapid Testing Two-times IRs
33
103
0.1%
16
15.5%
84
81.6%
3
2.9 %
3
2.9 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
87
0.0%
4
4.6%
81
93.1%
3
3.4 %
2
2.3 %
0
Parallel New Infection
21
679
0.3%
16
2.4%
625
92.0%
31
4.6 %
22
3.2 %
16
2.4 %
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales
859
39142
19.3%
2014
5.1%
35027
89.5%
2437
6.2 %
1926
4.9 %
178
0.5 %
Scotland - Scotland
Parallel New Infection
13
1856
0.9%
81
4.4%
1702
91.7%
88
4.7 %
73
3.9 %
0
Scotland
13
1856
0.9%
81
4.4%
1702
91.7%
88
4.7 %
73
3.9 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | August  |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| April        | 269     |
| May          | 364     |
| June         | 287     |
| July         | 316     |
| Totals (last |         |
| 12 months)   |         |
| 3388         | 313834  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1965
246558
1312
11288
321
0
0
0
0
923
12
12
34
4
9827
16
96
0
2
Scotland
17
1924
10
84
1
0
0
0
0
12
0
0
2
0
54
0
4
0
0
Wales
1406
65352
718
3664
77
0
0
0
0
152
8
0
19
3
2675
100
580
3
26
Totals
3388
313834
2040
15036
399
0
0
0
0
1087
20
12
55
7
12556
116
680
3
28

*Includes all animals with IFNg test negative, resample and reject outcomes

| County        | %Resampled %Retested   |
|---------------|------------------------|
| Argyll & Bute |                        |
| Avon          | 3.92 %                 |
| Berks         | 8.63 %                 |
| Bucks         | 10.86 %                |
| Cambs         | 6.47 %                 |
| Carms         | 2.29 %                 |
| Ceredigion    | 3.24 %                 |
| Cheshire      | 3.79 %                 |
| Corn & Scilly | 4.84 %                 |
| Cumbria       | 6.27 %                 |
| Derbyshire    | 9.66 %                 |
| Devon         | 4.38 %                 |
| Dorset        | 6.39 %                 |
| Dumfries & G  | 2.55 %                 |
| E Sussex      | 14.22 %                |
| Gloucs        | 7.98 %                 |
| Gwent         | 3.07 %                 |
| Gwynedd       | 1.79 %                 |
| Hampshire     | 4.73 %                 |
| Heref         | 5.07 %                 |
| Kent          | 30.00 %                |
| Leics & Rut   | 5.43 %                 |
| NE Wales      | 2.76 %                 |
| Northants       | 4.62 %   | 10.77 %   |
|-----------------|----------|-----------|
| Oxon            | 5.68 %   | 8.74 %    |
| Pembs           | 2.60 %   | 3.91 %    |
| Powys           | 4.17 %   | 5.37 %    |
| S Glamorgan     | 2.63 %   | 5.26 %    |
| Shropshire      | 5.09 %   | 5.87 %    |
| Somerset excl N | 3.95 %   | 6.40 %    |
| S Wales         |          |           |
| S Yorks         |          |           |
| Warks           | 6.42 %   | 8.19 %    |
| Wigtown         | 8.15 %   | 10.03 %   |
| Wiltshire       | 4.56 %   | 6.05 %    |
| W Sussex   | 23.83 %   | 26.42 %   |
|------------|-----------|-----------|
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
24686
948
3.8%
25006
948
3.8%
Wales
6521
249
3.8%
6558
249
3.8%
Scotland
970
22
2.3%
985
22
2.2%
GB
32177
1219
3.8%
32549
1219
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
18799
620
3.3%
18982
620
3.3%
Wales
4628
167
3.6%
4774
167
3.5%
Scotland
336
19
5.7%
355
19
5.4%
GB
23761
806
3.4%
24111
806
3.3%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
151628
7075
4.7%
161696
7075
4.4%
Wales
36139
2014
5.6%
39142
2014
5.1%
Scotland
1829
81
4.4%
1856
81
4.4%
GB
189593
9170
4.8%
202694
9170
4.5%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
97365
5407
5.6%
108776
5407
5.0%
Wales
28831
1219
4.2%
31397
1219
3.9%
Scotland
2017
178
8.8%
2173
178
8.2%
GB
128200
6804
5.3%
142346
6804
4.8%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Somerset excl North
PRV-SP
3
3
0
0
Total
3
3
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Devon               | PRV-SE        |
| Gloucestershire     | PRV-SP        |
| Herefordshire       | PRV-SP        |
| Somerset excl North | PRV-SE        |
| PRV-SP              | 3             |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 12                  |               |
| 64                  |               |
| 15                  | 23.44 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |                31 | 3                    | 9.7%                              |
| North East Wales | 1               |       84 |                 3 | 3.6%                 |                                   |
| Pembrokeshire    | 3               |      130 |                 6 | 4.6%                 |                                   |
| Powys            | 1               |      249 |                11 | 4.4%                 |                                   |
| South Wales      | 1               |       32 |                 2 | 6.3%                 |                                   |
| TC0077           | Sum:            |      526 |                25 | 4.8 %                |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 8        |               281 | 22                   | 7.8%                              |
| Ceredigion       | 1               | 29       |                 7 | 24.1%                |                                   |
| North East Wales | 12              | 467      |                66 | 14.1%                |                                   |
| Pembrokeshire    | 12              | 395      |                21 | 5.3%                 |                                   |
| Powys            | 4               | 268      |                14 | 5.2%                 |                                   |
| South Wales      | 4               | 187      |                16 | 8.6%                 |                                   |
| TC0077           | Sum:            | 1,627    |               146 | 9.0 %                |                                   |