# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 165

# Report Period 1St - 31St May 2020

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2020               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| May 2020                |                          |          |
| Submissions             | 227                      | 1339     |
| Samples                 | Total (%)                | 17571    |
| England                 | 13263                    | 75.48 %  |
| Wales                   | 3825                     | 21.77 %  |
| Scotland                | 483                      | 2.75 %   |
| Parallel Tests          | PAR-CUL-N                | 6351     |
| PAR-CUL-P               | 170                      | 0.97 %   |
| PAR-HERD-S              | 75                       | 0.43 %   |
| PAR-NEW-IN              | 8318                     | 47.34 %  |
| PAR-OTHER               | 116                      | 0.66 %   |
| PAR-PERSIS              | 2244                     | 12.77 %  |
| PAR-RAPID               | 157                      | 0.89 %   |
| Total(% of all samples) | 17431                    | 99.20 %  |
| Serial/Extended Tests   | SER-FLEXI                | 140      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| Total(% of all samples) | 140                      | 0.80 %   |
| Total                   | 17571                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1025     |
| England                 | 786                      | 4.47 %   |
| Wales                   | 217                      | 1.23 %   |
| Scotland                | 22                       | 0.13 %   |
| Resamples               | Total (% of all samples) | 749      |
| England                 | 561                      | 3.19 %   |
| Wales                   | 170                      | 0.97 %   |
| Scotland                | 18                       | 0.10 %   |
| Rejects                 | Total (% of all samples) | 8        |
| England                 | 4                        | 0.02 %   |
| Wales                   | 4                        | 0.02 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

Reason for
| PAR-CUL-N   |   6351 | 36.1 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    170 | 1.0 %    |
| PAR-HERD-S  |     75 | 0.4 %    |
| PAR-NEW-IN  |   8318 | 47.3 %   |
| PAR-OTHER   |    116 | 0.7 %    |
| PAR-PERSIS  |   2244 | 12.8 %   |
| PAR-RAPID   |    157 | 0.9 %    |
| SER-FLEXI   |    140 | 0.8 %    |
| Total       |  17571 | 100.00 % |
Breakdown
of Samples
submitted
by County

(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For May 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 1              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| Dorset          | PAR-CUL-N      |
| Gloucs          | PAR-CUL-N      |
| PAR-PERSIS      | 2              |
| Heref           | PAR-CUL-N      |
| Somerset excl N | PAR-CUL-N      |
| PAR-PERSIS      | 1              |
| Staffs          | PAR-NEW-IN     |
| Wiltshire       | PAR-CUL-N      |
| England - Edge  | Berks          |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cleveland      |
| Lincs           | PAR-NEW-IN     |
| W Yorks         | PAR-NEW-IN     |
| England         |                |
| 115             | 13263          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 6              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 2              |
| Ceredigion              | PAR-OTHER      |
| PAR-RAPID               | 2              |
| Gwent                   | PAR-PERSIS     |
| Pembs                   | PAR-HERD-S     |
| PAR-OTHER               | 7              |
| PAR-PERSIS              | 10             |
| PAR-RAPID               | 15             |
| SER-FLEXI               | 1              |
| Powys                   | PAR-NEW-IN     |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 5              |
| Wales - Intermediate TB | Carms          |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 3              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| Powys                   | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 1              |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 104                     | 3825           |
| Scotland - Scotland     | Kirkcudbright  |
| PAR-PERSIS              | 1              |
| Perthshire              | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| Wigtown     | PAR-NEW-IN     |
| Scotland    |                |
| 8           | 483            |
| Grand Total | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-CUL-P       | 7              |
| PAR-PERSIS      | 17             |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 8              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 7              |
| PAR-NEW-IN      | 2              |
| SER-FLEXI       | 5              |
| Gloucs          | PAR-CUL-N      |
| PAR-PERSIS      | 2              |
| Heref           | PAR-CUL-N      |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-CUL-N      |
| PAR-PERSIS      | 7              |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 3              |
| Staffs          | PAR-CUL-N      |
| PAR-NEW-IN      | 12             |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 4              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-PERSIS      | 2              |
| SER-FLEXI       | 2              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 78             |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-CUL-N      |
| PAR-NEW-IN      | 9              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cleveland      |
| Cumbria         | PAR-NEW-IN     |
| Gr Manchester   | PAR-NEW-IN     |
| Humberside      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| Norfolk         | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| Surrey          | PAR-NEW-IN     |
| W Yorks         | PAR-NEW-IN     |
| England         |                |
| 730             | 86580          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 18             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2020 Cont.

| Country     | County         |
|-------------|----------------|
| Submission  |                |
| Reasons*    |                |
| No.         |                |
| subs        |                |
| Samples     | Gamma Positive |
| n           | % of total     |
| PAR-PERSIS  | 30             |
| PAR-RAPID   | 32             |
| SER-FLEXI   | 4              |
| Ceredigion  | PAR-HERD-S     |
| PAR-OTHER   | 13             |
| PAR-PERSIS  | 16             |
| PAR-RAPID   | 15             |
| Gwent       | PAR-HERD-S     |
| PAR-PERSIS  | 14             |
| PAR-RAPID   | 10             |
| SER-FLEXI   | 3              |
| Pembs       | PAR-HERD-S     |
| PAR-OTHER   | 43             |
| PAR-PERSIS  | 64             |
| PAR-RAPID   | 54             |
| SER-FLEXI   | 4              |
| Powys       | PAR-HERD-S     |
| PAR-NEW-IN  | 1              |
| PAR-OTHER   | 9              |
| PAR-PERSIS  | 35             |
| PAR-RAPID   | 18             |
| SER-FLEXI   | 10             |
| S Wales     | PAR-PERSIS     |
| PAR-RAPID   | 1              |
| SER-FLEXI   | 1              |
| W Glamorgan | PAR-OTHER      |
| PAR-PERSIS  | 2              |
| SER-FLEXI   | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| Wales - Intermediate TB | Carms          |
| PAR-OTHER               | 5              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 3              |
| Ceredigion              | PAR-PERSIS     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 4              |
| PAR-PERSIS              | 11             |
| PAR-RAPID               | 8              |
| SER-FLEXI               | 8              |
| Powys                   | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-RAPID               | 1              |
| S Glamorgan             | PAR-RAPID      |
| SER-FLEXI               | 3              |
| Wales - Low TB          | Gwynedd        |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| Wales                   |                |
| 575                     | 21415          |
| Scotland - Scotland     | Argyll & Bute  |
| Ayrshire                | PAR-NEW-IN     |
| Berwickshire            | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| Dumfries & G  | PAR-NEW-IN     |
| Kirkcudbright | PAR-NEW-IN     |
| PAR-PERSIS    | 1              |
| Perthshire    | PAR-NEW-IN     |
| Wigtown       | PAR-NEW-IN     |
| Scotland      |                |
| 34            | 3145           |
| Grand Total   | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 115          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 8            |
| Wales           | Parallel     |
| Serial/Extended | 4            |
| Total           | 104          |
| GB              | Parallel     |
| Serial/Extended | 4            |
| Total           | 227          |
| 115.00          |              |
| 104.00          |              |
| 8.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2020)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 13           |
| Total           | 730          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 34           |
| Wales           | Parallel     |
| Serial/Extended | 36           |
| Total           | 575          |
| GB              | Parallel     |
| Serial/Extended | 49           |
| Total           | 1339 111140  |
| 730.00          |              |
| 575.00          |              |
| 34.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (May 2020)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
4
140
0.8%
15
10.7%
120
85.7%
4
2.9 %
3
2.1 %
2
1.4 %
Parallel - Herd or Group Slaughter
2
75
0.4%
5
6.7%
62
82.7%
8
10.7 %
8
10.7 %
0
Parallel New Infection
91
8318
47.3%
271
3.3%
7650
92.0%
538
6.5 %
397
4.8 %
0
Parallel - new infection cull area
50
6351
36.1%
370
5.8%
5729
90.2%
348
5.5 %
248
3.9 %
4
0.1 %
Parallel - Other
21
116
0.7%
12
10.3%
102
87.9%
2
1.7 %
2
1.7 %
0
Parallel Persistent Infection
29
2244
12.8%
153
6.8%
2008
89.5%
108
4.8 %
81
3.6 %
2
0.1 %
Parallel - persistent infection cull area
2
170
1.0%
1
0.6%
163
95.9%
8
4.7 %
6
3.5 %
0
Parallel - Rapid Testing Two-times IRs
28
157
0.9%
31
19.7%
122
77.7%
9
5.7 %
4
2.5 %
0
Total
227
17571
100.0%
858
4.9%
15956
90.8%
1025
5.8 %
749
4.3 %
8
0.0 %
England - HRA
Parallel New Infection
12
1884
10.7%
56
3.0%
1766
93.7%
82
4.4 %
62
3.3 %
0
Parallel - new infection cull area
50
6351
36.1%
370
5.8%
5729
90.2%
348
5.5 %
248
3.9 %
4
0.1 %
Parallel Persistent Infection
3
249
1.4%
14
5.6%
225
90.4%
13
5.2 %
10
4.0 %
0
Parallel - persistent infection cull area
2
170
1.0%
1
0.6%
163
95.9%
8
4.7 %
6
3.5 %
0
England - HRA
67
8654
49.3%
441
5.1%
7883
91.1%
451
5.2 %
326
3.8 %
4
0.0 %
England - Edge
Parallel New Infection
44
4449
25.3%
166
3.7%
4054
91.1%
324
7.3 %
229
5.1 %
0
England - Edge
44
4449
25.3%
166
3.7%
4054
91.1%
324
7.3 %
229
5.1 %
0
England - LRA
Parallel New Infection
4
160
0.9%
9
5.6%
145
90.6%
11
6.9 %
6
3.8 %
0
England - LRA
4
160
0.9%
9
5.6%
145
90.6%
11
6.9 %
6
3.8 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
3
88
0.5%
14
15.9%
69
78.4%
3
3.4 %
3
3.4 %
2
2.3 %
Parallel - Herd or Group Slaughter
2
75
0.4%
5
6.7%
62
82.7%
8
10.7 %
8
10.7 %
0
Parallel New Infection
1
36
0.2%
2
5.6%
34
94.4%
0
0
0
Parallel - Other
16
108
0.6%
12
11.1%
95
88.0%
1
0.9 %
1
0.9 %
0
Parallel Persistent Infection
24
1905
10.8%
135
7.1%
1700
89.2%
91
4.8 %
68
3.6 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
26
147
0.8%
28
19.0%
115
78.2%
9
6.1 %
4
2.7 %
0
Wales - High TB
72
2359
13.4%
196
8.3%
2075
88.0%
112
4.7 %
84
3.6 %
4
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
52
0.3%
1
1.9%
51
98.1%
1
1.9 %
0
0
Parallel New Infection
17
1050
6.0%
32
3.0%
963
91.7%
67
6.4 %
55
5.2 %
0
Parallel - Other
5
8
0.0%
0
0.0 %
7
87.5%
1
12.5 %
1
12.5 %
0
Parallel Persistent Infection
1
68
0.4%
4
5.9%
61
89.7%
4
5.9 %
3
4.4 %
0
Parallel - Rapid Testing Two-times IRs
2
10
0.1%
3
30.0%
7
70.0%
0
0
0
Wales - Intermediate TB
26
1188
6.8%
40
3.4%
1089
91.7%
73
6.1 %
59
5.0 %
0
Wales - Low TB
Parallel New Infection
6
278
1.6%
4
1.4%
247
88.8%
32
11.5 %
27
9.7 %
0
Wales - Low TB
6
278
1.6%
4
1.4%
247
88.8%
32
11.5 %
27
9.7 %
0
Scotland - Scotland
Parallel New Infection
7
461
2.6%
2
0.4%
441
95.7%
22
4.8 %
18
3.9 %
0
Parallel Persistent Infection
1
22
0.1%
0
0.0 %
22
100.0%
0
0
0
Scotland - Scotland
8
483
2.7%
2
0.4%
463
95.9%
22
4.6 %
18
3.7 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
49
2008
1.8%
144
7.2%
1793
89.3%
108
5.4 %
67
3.3 %
4
0.2 %
Parallel - Herd or Group Slaughter
23
382
0.3%
34
8.9%
330
86.4%
29
7.6 %
18
4.7 %
0
Parallel New Infection
467
51133
46.0%
1468
2.9%
45827
89.6%
3231
6.3 %
2564
5.0 %
1274
2.5 %
Parallel - new infection cull area
315
35395
31.8%
1550
4.4%
31743
89.7%
2132
6.0 %
1639
4.6 %
463
1.3 %
Parallel - Other
97
1871
1.7%
164
8.8%
1573
84.1%
155
8.3 %
134
7.2 %
0
Parallel Persistent Infection
214
14219
12.8%
869
6.1%
12601
88.6%
925
6.5 %
725
5.1 %
24
0.2 %
Parallel - persistent infection cull area
27
5534
5.0%
333
6.0%
4951
89.5%
336
6.1 %
249
4.5 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
147
598
0.5%
124
20.7%
443
74.1%
39
6.5 %
31
5.2 %
0
Total
1339 111140
100.0%
4686
4.2%
99261
89.3%
6955
6.3 %
5427
4.9 %
1766
1.6 %
England - HRA
Extended Flexible IFN-GAMMA test
13
1224
1.1%
70
5.7%
1113
90.9%
59
4.8 %
41
3.3 %
0
Parallel New Infection
17
2299
2.1%
71
3.1%
2129
92.6%
131
5.7 %
98
4.3 %
1
0.0 %
Parallel - new infection cull area
311
34981
31.5%
1534
4.4%
31374
89.7%
2083
6.0 %
1612
4.6 %
461
1.3 %
Parallel Persistent Infection
34
5291
4.8%
256
4.8%
4801
90.7%
307
5.8 %
231
4.4 %
3
0.1 %
Parallel - persistent infection cull area
27
5534
5.0%
333
6.0%
4951
89.5%
336
6.1 %
249
4.5 %
1
0.0 %
England - Edge
Parallel New Infection
295
34892
31.4%
1056
3.0%
31192
89.4%
2223
6.4 %
1754
5.0 %
890
2.6 %
Parallel - new infection cull area
4
414
0.4%
16
3.9%
369
89.1%
49
11.8 %
27
6.5 %
2
0.5 %
England - LRA
Parallel New Infection
29
1945
1.8%
49
2.5%
1509
77.6%
93
4.8 %
73
3.8 %
314
16.1 %
England
730
86580
77.9%
3385
3.9%
77438
89.4%
5281
6.1 %
4085
4.7 %
1672
1.9 %
Wales - High TB
Extended Flexible IFN-GAMMA test
23
542
0.5%
56
10.3%
467
86.2%
34
6.3 %
17
3.1 %
2
0.4 %
Parallel - Herd or Group Slaughter
20
352
0.3%
29
8.2%
306
86.9%
26
7.4 %
17
4.8 %
0
Parallel New Infection
2
44
0.0%
4
9.1%
40
90.9%
0
0
0
Parallel - Other
84
1812
1.6%
159
8.8%
1524
84.1%
148
8.2 %
129
7.1 %
0
Parallel Persistent Infection
162
8116
7.3%
521
6.4%
7115
87.7%
575
7.1 %
459
5.7 %
21
0.3 %
Parallel - Rapid Testing Two-times IRs
130
544
0.5%
114
21.0%
404
74.3%
34
6.3 %
26
4.8 %
0
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
13
242
0.2%
18
7.4%
213
88.0%
15
6.2 %
9
3.7 %
2
0.8 %
Parallel - Herd or Group Slaughter
3
30
0.0%
5
16.7%
24
80.0%
3
10.0 %
1
3.3 %
0

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2020) Cont.

Parallel New Infection
60
5899
5.3%
175
3.0%
5365
90.9%
418
7.1 %
358
6.1 %
1
0.0 %
Parallel - Other
11
53
0.0%
5
9.4%
44
83.0%
6
11.3 %
4
7.5 %
0
Parallel Persistent Infection
16
750
0.7%
92
12.3%
631
84.1%
33
4.4 %
27
3.6 %
0
Parallel - Rapid Testing Two-times IRs
14
41
0.0%
10
24.4%
29
70.7%
2
4.9 %
2
4.9 %
0
Wales - Low TB
Parallel New Infection
31
2931
2.6%
36
1.2%
2755
94.0%
197
6.7 %
137
4.7 %
3
0.1 %
Parallel - Other
2
6
0.0%
0
0.0 %
5
83.3%
1
16.7 %
1
16.7 %
0
Parallel Persistent Infection
1
40
0.0%
0
0.0 %
32
80.0%
10
25.0 %
8
20.0 %
0
Parallel - Rapid Testing Two-times IRs
3
13
0.0%
0
0.0 %
10
76.9%
3
23.1 %
3
23.1 %
0
Wales
575
21415
19.3%
1224
5.7%
18964
88.6%
1505
7.0 %
1198
5.6 %
29
0.1 %
Scotland - Scotland
Parallel New Infection
33
3123
2.8%
77
2.5%
2837
90.8%
169
5.4 %
144
4.6 %
65
2.1 %
Parallel Persistent Infection
1
22
0.0%
0
0.0 %
22
100.0%
0
0
0
Scotland
34
3145
2.8%
77
2.4%
2859
90.9%
169
5.4 %
144
4.6 %
65
2.1 %
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2019         | June    |
| July         | 316     |
| August       | 262     |
| September    | 245     |
| October      | 298     |
| November     | 272     |
| December     | 234     |
| 2020         | January |
| February     | 323     |
| March        | 262     |
| April        | 184     |
| May          | 227     |
| Totals (last |         |
| 12 months)   |         |
| 3253         | 299248  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1869
235480
1156
8028
405
0
0
0
0
978
8
2
43
3
6295
0
38
0
1
Scotland
49
4979
24
109
3
0
0
0
0
12
0
0
2
0
73
3
12
0
3
Wales
1335
58789
690
2876
101
0
0
0
0
125
10
3
23
2
1906
50
540
0
7
Totals
3253
299248
1870
11013
509
0
0
0
0
1115
18
5
68
5
8274
53
590
0
11

*Includes all animals with IFNg test negative, resample and reject outcomes Cheshire Cleveland Derbyshire Devon Dorset E Sussex Gloucs Gwent Gwynedd Hampshire Heref Kirkcudbright Leics & Rut Lincs Oxon Pembs Powys

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Berks         | 11.11 %  | 14.81 %                |
| Carms         | 4.42 %   | 6.02 %                 |
| Ceredigion    | 4.55 %   | 4.55 %                 |
| Cheshire      | 4.52 %   | 6.30 %                 |
| Cleveland     | 2.00 %   |                        |
| Corn & Scilly | 4.26 %   | 5.98 %                 |
| Derbyshire    | 6.75 %   | 8.51 %                 |
| Devon         | 5.24 %   | 7.21 %                 |
| Dorset        | 2.36 %   | 3.31 %                 |
| E Sussex      | 7.11 %   | 9.48 %                 |
| Gloucs        | 5.73 %   | 6.49 %                 |
| Gwent         | 1.31 %   | 2.06 %                 |
| Gwynedd       | 3.77 %   | 3.77 %                 |
| Hampshire     | 3.79 %   | 8.20 %                 |
| Heref         | 2.65 %   | 3.54 %                 |
| Kirkcudbright | 4.17 %   | 5.83 %                 |
| Leics & Rut   |          |                        |
| Lincs         | 4.23 %   | 5.63 %                 |
| NE Wales      | 6.03 %   | 7.60 %                 |
| Oxon          | 4.68 %   | 7.12 %                 |
| Pembs         | 4.52 %   | 5.46 %                 |
| Perthshire    | 3.36 %   | 3.92 %                 |
| Powys         | 4.02 %   | 5.02 %                 |
| Somerset excl N   | 1.69 %   | 2.75 %   |
|-------------------|----------|----------|
| Staffs            | 3.29 %   | 4.35 %   |
| Warks             | 2.27 %   | 2.27 %   |
| Wigtown           | 16.67 %  | 16.67 %  |
| Wiltshire         | 6.55 %   | 9.48 %   |
| W Yorks           | 7.69 %   | 15.38 %  |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
13105
616
4.7%
13263
616
4.6%
Wales
3759
240
6.4%
3825
240
6.3%
Scotland
479
2
0.4%
483
2
0.4%
GB
17343
858
4.9%
17571
858
4.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23289
864
3.7%
23479
864
3.7%
Wales
4302
198
4.6%
4385
198
4.5%
Scotland
493
54
11.0%
493
54
11.0%
GB
28083
1116
4.0%
28357
1116
3.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
81721
3385
4.1%
86580
3385
3.9%
Wales
20339
1223
6.0%
21415
1224
5.7%
Scotland
2990
77
2.6%
3145
77
2.4%
GB
105049
4685
4.5%
111140
4686
4.2%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
108872
5383
4.9%
114874
5383
4.7%
Wales
26100
1546
5.9%
28125
1546
5.5%
Scotland
859
58
6.8%
859
58
6.8%
GB
135830
6987
5.1%
143858
6987
4.9%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
| % Positive      | * Test type   |
|-----------------|---------------|
| Num             |               |
| Submissions     |               |
| Num             |               |
| Samples         |               |
| Num             |               |
| Positives       |               |
| Private         |               |
| Cheshire        | PRV-SP        |
| Devon           | PRV-SE        |
| PRV-SP          | 1             |
| Dorset          | PRV-SE        |
| PRV-SP          | 1             |
| Gloucestershire | PRV-SE        |
| Oxfordshire     | PRV-SP        |
| Staffordshire   | PRV-SE        |
| Worcestershire  | PRV-SP        |
| Total           |               |
| 9               |               |
| 195             |               |
| 3               | 1.54 %        |
| Isle of Man     | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |                23 | 7                    | 30.4%                             |
| Ceredigion       | 3               |       14 |                 2 | 14.3%                |                                   |
| North East Wales | 4               |       15 |                 2 | 13.3%                |                                   |
| Pembrokeshire    | 15              |      175 |                34 | 19.4%                |                                   |
| Powys            | 5               |       58 |                11 | 19.0%                |                                   |
| Wiltshire        | 1               |      291 |                 3 | 1.0%                 |                                   |
| TC0077           | 30              |      576 |                59 | 10.2 %               |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 14       |               268 | 44                   | 16.4%                             |
| Ceredigion       | 10              | 40       |                 6 | 15.0%                |                                   |
| Devon            | 4               | 1,032    |                95 | 9.2%                 |                                   |
| North East Wales | 18              | 530      |                57 | 10.8%                |                                   |
| Pembrokeshire    | 43              | 618      |               112 | 18.1%                |                                   |
| Powys            | 9               | 161      |                24 | 14.9%                |                                   |
| South Wales      | 10              | 149      |                34 | 22.8%                |                                   |
| Wiltshire        | 1               | 291      |                 3 | 1.0%                 |                                   |
| TC0077           | 109             | 3,089    |               375 | 12.1 %               |                                   |