# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number Report Period

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |
| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| January                 |                          |          |
| 2019                    |                          |          |
| Submissions             | 289                      | 289      |
| Samples                 | Total (%)                | 27513    |
| England                 | 20585                    | 74.82 %  |
| Wales                   | 6928                     | 25.18 %  |
| Scotland                |                          |          |
| Parallel Tests          | PAR-CUL-N                | 2848     |
| PAR-CUL-P               | 2527                     | 9.18 %   |
| PAR-HERD-S              | 634                      | 2.30 %   |
| PAR-NEW-IN              | 13956                    | 50.73 %  |
| PAR-OTHER               | 1605                     | 5.83 %   |
| PAR-PERSIS              | 5313                     | 19.31 %  |
| PAR-RAPID               | 173                      | 0.63 %   |
| Total(% of all samples) | 27056                    | 98.34 %  |
| Serial/Extended Tests   | SER-FLEXI                | 450      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 7                        | 0.03 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 457                      | 1.66 %   |
| Total                   | 27513                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1937     |
| England                 | 1526                     | 5.55 %   |
| Wales                   | 411                      | 1.49 %   |
| Scotland                |                          |          |
| Resamples               | Total (% of all samples) | 1657     |
| England                 | 1335                     | 4.85 %   |
| Wales                   | 322                      | 1.17 %   |
| Scotland                |                          |          |
| Rejects                 | Total (% of all samples) | 974      |
| England                 | 860                      | 3.13 %   |
| Wales                   | 114                      | 0.41 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   2848 | 10.4 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   2527 | 9.2 %    |
| PAR-HERD-S  |    634 | 2.3 %    |
| PAR-NEW-IN  |  13956 | 50.7 %   |
| PAR-OTHER   |   1605 | 5.8 %    |
| PAR-PERSIS  |   5313 | 19.3 %   |
| PAR-RAPID   |    173 | 0.6 %    |
| SER-FLEXI   |    450 | 1.6 %    |
| SER-NSR     |      7 | 0.0 %    |
| Total       |  27513 | 100.00 % |
| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Avon           |
| Corn & Scilly  | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-PERSIS     | 2              |
| SER-FLEXI      | 2              |
| Devon          | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-NEW-IN     | 1              |
| PAR-PERSIS     | 3              |
| SER-FLEXI      | 2              |
| Dorset         | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| Gloucs         | PAR-CUL-N      |
| Heref          | PAR-CUL-N      |
| Shropshire     | PAR-OTHER      |
| PAR-PERSIS     | 1              |
| Somerset       | PAR-CUL-P      |
| PAR-OTHER      | 2              |
| Wiltshire      | PAR-OTHER      |
| SER-FLEXI      | 1              |
| England - Edge | Berks          |
| PAR-PERSIS     | 1              |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| PAR-PERSIS     | 1              |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| Hampshire      | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For January 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| SER-NSR         | 1              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 162             | 20585          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 17             |
| PAR-RAPID       | 11             |
| SER-FLEXI       | 4              |
| Ceredigion      | PAR-PERSIS     |
| PAR-RAPID       | 4              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 8              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 5              |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 13             |
| SER-FLEXI       | 1              |
| Powys           | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For January 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-NEW-IN              | 2              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-HERD-S     |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| Wales - Intermediate TB | Carms          |
| Ceredigion              | PAR-HERD-S     |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 8              |
| W Glamorgan             | PAR-HERD-S     |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 127                     | 6928           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| England - HRA  | Avon           |
| Corn & Scilly  | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-PERSIS     | 2              |
| SER-FLEXI      | 2              |
| Devon          | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| PAR-NEW-IN     | 1              |
| PAR-PERSIS     | 3              |
| SER-FLEXI      | 2              |
| Dorset         | PAR-CUL-N      |
| PAR-CUL-P      | 2              |
| Gloucs         | PAR-CUL-N      |
| Heref          | PAR-CUL-N      |
| Shropshire     | PAR-OTHER      |
| PAR-PERSIS     | 1              |
| Somerset       | PAR-CUL-P      |
| PAR-OTHER      | 2              |
| Wiltshire      | PAR-OTHER      |
| SER-FLEXI      | 1              |
| England - Edge | Berks          |
| PAR-PERSIS     | 1              |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-NEW-IN     |
| PAR-PERSIS     | 1              |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| Hampshire      | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| SER-NSR         | 1              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 162             | 20585          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 17             |
| PAR-RAPID       | 11             |
| SER-FLEXI       | 4              |
| Ceredigion      | PAR-PERSIS     |
| PAR-RAPID       | 4              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 8              |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 5              |
| PAR-PERSIS      | 6              |
| PAR-RAPID       | 13             |
| SER-FLEXI       | 1              |
| Powys           | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-NEW-IN              | 2              |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 4              |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-HERD-S     |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| Wales - Intermediate TB | Carms          |
| Ceredigion              | PAR-HERD-S     |
| NE Wales                | PAR-NEW-IN     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 8              |
| W Glamorgan             | PAR-HERD-S     |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 127                     | 6928           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol January 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 6            |
| Total           | 162          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 15           |
| Total           | 127          |
| GB              | Parallel     |
| Serial/Extended | 21           |
| Total           | 289          |
| 162.00          |              |
| 127.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 6            |
| Total           | 162          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 15           |
| Total           | 127          |
| GB              | Parallel     |
| Serial/Extended | 21           |
| Total           | 289          |
| 162.00          |              |
| 127.00          |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
450
1.6%
33
7.3%
316
70.2%
17
3.8 %
17
3.8 %
84
18.7 %
Parallel - Herd or Group Slaughter
16
634
2.3%
50
7.9%
563
88.8%
31
4.9 %
19
3.0 %
2
0.3 %
Parallel New Infection
119
13956
50.7%
534
3.8%
11923
85.4%
1004
7.2 %
888
6.4 %
611
4.4 %
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
11
1605
5.8%
91
5.7%
1359
84.7%
132
8.2 %
114
7.1 %
41
2.6 %
Parallel Persistent Infection
46
5313
19.3%
271
5.1%
4668
87.9%
322
6.1 %
262
4.9 %
112
2.1 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
Parallel - Rapid Testing Two-times IRs
41
173
0.6%
50
28.9%
95
54.9%
17
9.8 %
10
5.8 %
18
10.4 %
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0 %
1657
6.0 %
974
3.5 %
England - HRA
Extended Flexible IFN-GAMMA test
5
79
0.3%
6
7.6%
41
51.9%
1
1.3 %
1
1.3 %
31
39.2 %
Parallel New Infection
1
344
1.3%
67
19.5%
242
70.3%
42
12.2 %
35
10.2 %
0
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
5
728
2.6%
59
8.1%
580
79.7%
56
7.7 %
48
6.6 %
41
5.6 %
Parallel Persistent Infection
8
774
2.8%
40
5.2%
564
72.9%
78
10.1 %
73
9.4 %
97
12.5 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
England - HRA
54
7300
26.5%
426
5.8%
6095
83.5%
591
8.1 %
504
6.9 %
275
3.8 %
England - Edge
Parallel New Infection
96
12762
46.4%
437
3.4%
10944
85.8%
896
7.0 %
796
6.2 %
585
4.6 %
Parallel Persistent Infection
4
262
1.0%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - Edge
101
13031
47.4%
451
3.5%
11187
85.8%
910
7.0 %
808
6.2 %
585
4.5 %
England - LRA
Parallel New Infection
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
England - LRA
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
7
123
0.4%
8
6.5%
109
88.6%
6
4.9 %
6
4.9 %
0
Parallel - Herd or Group Slaughter
14
627
2.3%
48
7.7%
559
89.2%
30
4.8 %
18
2.9 %
2
0.3 %
Parallel New Infection
2
7
0.0%
0
0.0 %
5
71.4%
2
28.6 %
2
28.6 %
0
Parallel - Other
5
857
3.1%
30
3.5%
767
89.5%
70
8.2 %
60
7.0 %
0
Parallel Persistent Infection
33
4271
15.5%
217
5.1%
3862
90.4%
230
5.4 %
177
4.1 %
15
0.4 %
Parallel - Rapid Testing Two-times IRs
40
171
0.6%
50
29.2%
93
54.4%
17
9.9 %
10
5.8 %
18
10.5 %
Wales - High TB
101
6056
22.0%
353
5.8%
5395
89.1%
355
5.9 %
273
4.5 %
35
0.6 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
248
0.9%
19
7.7%
166
66.9%
10
4.0 %
10
4.0 %
53
21.4 %
Parallel - Herd or Group Slaughter
2
7
0.0%
2
28.6%
4
57.1%
1
14.3 %
1
14.3 %
0
Parallel New Infection
7
515
1.9%
18
3.5%
456
88.5%
37
7.2 %
31
6.0 %
10
1.9 %
Parallel - Other
1
20
0.1%
2
10.0%
12
60.0%
6
30.0 %
6
30.0 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Intermediate TB
20
798
2.9%
41
5.1%
646
81.0%
54
6.8 %
48
6.0 %
63
7.9 %
Wales - Low TB
Parallel New Infection
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %
Wales - Low TB
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
20
450
1.6%
33
7.3%
316
70.2%
17
3.8 %
17
3.8 %
84
18.7 %
Parallel - Herd or Group Slaughter
16
634
2.3%
50
7.9%
563
88.8%
31
4.9 %
19
3.0 %
2
0.3 %
Parallel New Infection
119
13956
50.7%
534
3.8%
11923
85.4%
1004
7.2 %
888
6.4 %
611
4.4 %
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
11
1605
5.8%
91
5.7%
1359
84.7%
132
8.2 %
114
7.1 %
41
2.6 %
Parallel Persistent Infection
46
5313
19.3%
271
5.1%
4668
87.9%
322
6.1 %
262
4.9 %
112
2.1 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
Parallel - Rapid Testing Two-times IRs
41
173
0.6%
50
28.9%
95
54.9%
17
9.8 %
10
5.8 %
18
10.4 %
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
289
27513
100.0%
1283
4.7%
23599
85.8%
1937
7.0 %
1657
6.0 %
974
3.5 %
England - HRA
Extended Flexible IFN-GAMMA test
5
79
0.3%
6
7.6%
41
51.9%
1
1.3 %
1
1.3 %
31
39.2 %
Parallel New Infection
1
344
1.3%
67
19.5%
242
70.3%
42
12.2 %
35
10.2 %
0
Parallel - new infection cull area
28
2848
10.4%
160
5.6%
2403
84.4%
212
7.4 %
183
6.4 %
102
3.6 %
Parallel - Other
5
728
2.6%
59
8.1%
580
79.7%
56
7.7 %
48
6.6 %
41
5.6 %
Parallel Persistent Infection
8
774
2.8%
40
5.2%
564
72.9%
78
10.1 %
73
9.4 %
97
12.5 %
Parallel - persistent infection cull area
7
2527
9.2%
94
3.7%
2265
89.6%
202
8.0 %
164
6.5 %
4
0.2 %
England - Edge
Parallel New Infection
96
12762
46.4%
437
3.4%
10944
85.8%
896
7.0 %
796
6.2 %
585
4.6 %
Parallel Persistent Infection
4
262
1.0%
14
5.3%
236
90.1%
14
5.3 %
12
4.6 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - LRA
Parallel New Infection
7
254
0.9%
12
4.7%
219
86.2%
25
9.8 %
23
9.1 %
0
England
162
20585
74.8%
889
4.3%
17501
85.0%
1526
7.4 %
1335
6.5 %
860
4.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
7
123
0.4%
8
6.5%
109
88.6%
6
4.9 %
6
4.9 %
0
Parallel - Herd or Group Slaughter
14
627
2.3%
48
7.7%
559
89.2%
30
4.8 %
18
2.9 %
2
0.3 %
Parallel New Infection
2
7
0.0%
0
0.0 %
5
71.4%
2
28.6 %
2
28.6 %
0
Parallel - Other
5
857
3.1%
30
3.5%
767
89.5%
70
8.2 %
60
7.0 %
0
Parallel Persistent Infection
33
4271
15.5%
217
5.1%
3862
90.4%
230
5.4 %
177
4.1 %
15
0.4 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Parallel - Rapid Testing Two-times IRs
40
171
0.6%
50
29.2%
93
54.4%
17
9.9 %
10
5.8 %
18
10.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
8
248
0.9%
19
7.7%
166
66.9%
10
4.0 %
10
4.0 %
53
21.4 %
Parallel - Herd or Group Slaughter
2
7
0.0%
2
28.6%
4
57.1%
1
14.3 %
1
14.3 %
0
Parallel New Infection
7
515
1.9%
18
3.5%
456
88.5%
37
7.2 %
31
6.0 %
10
1.9 %
Parallel - Other
1
20
0.1%
2
10.0%
12
60.0%
6
30.0 %
6
30.0 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
1
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales - Low TB
Parallel New Infection
6
74
0.3%
0
0.0 %
57
77.0%
2
2.7 %
1
1.4 %
16
21.6 %
Wales
127
6928
25.2%
394
5.7%
6098
88.0%
411
5.9 %
322
4.6 %
114
1.6 %
| Year         | Month    |
|--------------|----------|
| Herds        |          |
| sampled      |          |
| Samples      |          |
| tested       |          |
| IFNg+        |          |
| samples      |          |
| Wrong        |          |
| Eartags      |          |
| VL No        |          |
| Cult         |          |
| Herds        |          |
| with         |          |
| positives    |          |
| No PM        |          |
| No           |          |
| Cult         |          |
| No PM        |          |
| Cult         |          |
| Pend         |          |
| No PM        |          |
| Cult         |          |
| Neg          |          |
| No PM        |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Neg          |          |
| VL           |          |
| Cult         |          |
| Pend         |          |
| VL           |          |
| Cult         |          |
| Mb           |          |
| VL           |          |
| Cult         |          |
| Other        |          |
| NVL          |          |
| No           |          |
| Cult         |          |
| NVL          |          |
| Cult         |          |
| Pend         |          |
| NVL          |          |
| Cult         |          |
| Neg          |          |
| NVL          |          |
| Cult         |          |
| Mb           |          |
| NVL          |          |
| Cult         |          |
| Other        |          |
| No           |          |
| PM           |          |
| Cult         |          |
| Other        |          |
| 2018         | February |
| March        | 189      |
| April        | 195      |
| May          | 250      |
| June         | 187      |
| July         | 207      |
| August       | 228      |
| September    | 216      |
| October      | 274      |
| November     | 282      |
| December     | 228      |
| 2019         | January  |
| Totals (last |          |
| 12 months)   |          |
| 2751         | 261321   |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1530
200003
1049
9852
271
0
0
0
0
775
15
7
34
4
8193
25
51
1
3
Scotland
26
1828
17
146
30
0
0
0
0
22
4
0
0
0
49
0
36
0
3
Wales
1195
59490
600
2986
211
0
0
0
0
133
6
9
18
2
1828
169
393
3
17
Totals
2751
261321
1666
12984
512
0
0
0
0
930
25
16
52
6
10070
194
480
4
23

*Includes all animals with IFNg test negative, resample and reject outcomes

County
%Resampled %Retested
| Avon          | 8.50 %   | 10.00 %   |
|---------------|----------|-----------|
| Berks         | 5.99 %   | 6.72 %    |
| Bucks         | 1.96 %   | 4.58 %    |
| Cambs         | 28.57 %  | 28.57 %   |
| Carms         | 3.76 %   | 5.09 %    |
| Ceredigion    | 4.73 %   | 5.99 %    |
| Cheshire      | 5.31 %   | 6.27 %    |
| Corn & Scilly | 7.19 %   | 8.91 %    |
| Cumbria       | 33.33 %  | 33.33 %   |
| Derbyshire    | 4.79 %   | 5.51 %    |
| Devon         | 7.21 %   | 8.38 %    |
| Dorset        | 6.09 %   | 6.26 %    |
| E Sussex      | 3.59 %   | 3.72 %    |
| Gloucs        | 7.10 %   | 8.55 %    |
| Gwent         | 6.78 %   | 8.18 %    |
| Gwynedd       | 3.57 %   | 3.57 %    |
| Hampshire     | 4.84 %   | 4.84 %    |
| Heref         | 4.96 %   | 5.25 %    |
| Lancashire    | 4.55 %   | 4.55 %    |
| Leics & Rut   | 10.53 %  | 11.37 %   |
| Lincs         | 18.33 %  | 18.33 %   |
| NE Wales      | 5.02 %   | 5.88 %    |
| Northants     | 16.67 %  | 33.33 %   |
| Notts       | 10.61 %   | 11.67 %   |
|-------------|-----------|-----------|
| Oxon        | 10.06 %   | 10.66 %   |
| Pembs       | 6.20 %    | 7.47 %    |
| Powys       | 5.17 %    | 6.64 %    |
| Shropshire  | 9.89 %    | 11.22 %   |
| Somerset    | 3.65 %    | 5.36 %    |
| S Wales     |           |           |
| S Yorks     | 4.94 %    | 6.17 %    |
| Warks       | 6.54 %    | 7.11 %    |
| W Glamorgan | 3.08 %    | 5.38 %    |
| Wiltshire   | 7.89 %    | 13.16 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
14147
657
4.6%
14220
657
4.6%
Wales
4813
277
5.8%
5045
277
5.5%
Scotland
410
35
8.5%
413
35
8.5%
GB
19370
969
5.0%
19678
969
4.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
20087
889
4.4%
20585
889
4.3%
Wales
6848
394
5.8%
6928
394
5.7%
GB
26935
1283
4.8%
27513
1283
4.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
14147
657
4.6%
14220
657
4.6%
Wales
4813
277
5.8%
5045
277
5.5%
Scotland
410
35
8.5%
413
35
8.5%
GB
19370
969
5.0%
19678
969
4.9%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Gloucestershire
PRV-SP
1
4
0
Somerset excl North
PRV-SE
1
4
1
25.00 %
Wiltshire
PRV-SP
1
3
0
Total
3
11
1
9.09 %
Isle of Man
SER-IOM
3
19
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Gloucestershire     | PRV-SP        |
| Somerset excl North | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Total               |               |
| 3                   |               |
| 11                  |               |
| 1                   | 9.09 %        |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|        | Test             |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|--------|------------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077 | North East Wales |        2 |                97 | 5                    | 5.2 %                             |
| TC0077 | Sum:             |       97 |                 5 | 5.2 %                |                                   |

## Table 8B Number Of Cattle Antibody Tests 2019

|        | Test             |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|--------|------------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077 | North East Wales |        2 |                97 | 5                    | 5.2 %                             |
| TC0077 | Sum:             |       97 |                 5 | 5.2 %                |                                   |