# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 151

# Report Period 1St - 31St March 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |

## Table 1. Monthly Totals For March 2019

| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| March 2019              |                          |          |
| Submissions             | 315                      | 924      |
| Samples                 | Total (%)                | 29624    |
| England                 | 24319                    | 82.09 %  |
| Wales                   | 4939                     | 16.67 %  |
| Scotland                | 366                      | 1.24 %   |
| Parallel Tests          | PAR-CUL-N                | 6914     |
| PAR-CUL-P               | 1713                     | 5.78 %   |
| PAR-HERD-S              | 853                      | 2.88 %   |
| PAR-NEW-IN              | 14978                    | 50.56 %  |
| PAR-OTHER               | 504                      | 1.70 %   |
| PAR-PERSIS              | 4252                     | 14.35 %  |
| PAR-RAPID               | 127                      | 0.43 %   |
| Total(% of all samples) | 29341                    | 99.04 %  |
| Serial/Extended Tests   | SER-FLEXI                | 283      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 283                      | 0.96 %   |
| Total                   | 29624                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2100     |
| England                 | 1750                     | 5.91 %   |
| Wales                   | 330                      | 1.11 %   |
| Scotland                | 20                       | 0.07 %   |
| Resamples               | Total (% of all samples) | 1710     |
| England                 | 1413                     | 4.77 %   |
| Wales                   | 280                      | 0.95 %   |
| Scotland                | 17                       | 0.06 %   |
| Rejects                 | Total (% of all samples) | 61       |
| England                 | 58                       | 0.20 %   |
| Wales                   | 3                        | 0.01 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   6914 | 23.3 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   1713 | 5.8 %    |
| PAR-HERD-S  |    853 | 2.9 %    |
| PAR-NEW-IN  |  14978 | 50.6 %   |
| PAR-OTHER   |    504 | 1.7 %    |
| PAR-PERSIS  |   4252 | 14.4 %   |
| PAR-RAPID   |    127 | 0.4 %    |
| SER-FLEXI   |    283 | 1.0 %    |
| Total       |  29624 | 100.00 % |
of Samples
submitted
by County

## Table 2A.  Summary By County For March 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Corn & Scilly  |
| PAR-PERSIS      | 4              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Gloucestershire | PAR-CUL-N      |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Heref           | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 3              |
| Somerset excl N | PAR-CUL-N      |
| Staffs          | PAR-PERSIS     |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| PAR-NEW-IN      | 1              |
| England - Edge  | Berks          |
| Berkshire       | PAR-NEW-IN     |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 28             |
| PAR-PERSIS      | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cambs          |
| Gr Manchester   | PAR-NEW-IN     |
| I of Wight      | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 181             | 24319          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 5              |
| PAR-RAPID       | 9              |
| SER-FLEXI       | 3              |
| Ceredigion      | PAR-OTHER      |
| PAR-RAPID       | 4              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 1              |
| PAR-RAPID       | 3              |
| Pembrokeshire   | PAR-RAPID      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 10             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 20             |
| SER-FLEXI               | 1              |
| Powys                   | PAR-HERD-S     |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-PERSIS     |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-HERD-S     |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-RAPID               | 2              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-NEW-IN     |
| W Glamorgan             | PAR-RAPID      |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 131                     | 4939           |
| Scotland - Scotland     | Ayrshire       |
| Scotland                |                |
| 3                       | 366            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| SER-FRAUD       | 1              |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-PERSIS      | 6              |
| SER-FLEXI       | 2              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 14             |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 12             |
| SER-FLEXI       | 4              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 5              |
| Gloucestershire | PAR-CUL-N      |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Heref           | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-PERSIS      | 4              |
| Shropshire      | PAR-NEW-IN     |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 4              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| Staffs          | PAR-PERSIS     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country        | County         |
|----------------|----------------|
| Submission     |                |
| Reasons*       |                |
| No.            |                |
| subs           |                |
| Samples        | Gamma Positive |
| n              | % of total     |
| Wiltshire      | PAR-CUL-N      |
| PAR-CUL-P      | 3              |
| PAR-NEW-IN     | 1              |
| PAR-OTHER      | 1              |
| PAR-PERSIS     | 1              |
| SER-FLEXI      | 1              |
| England - Edge | Berks          |
| PAR-PERSIS     | 1              |
| Berkshire      | PAR-NEW-IN     |
| Bucks          | PAR-NEW-IN     |
| Cheshire       | PAR-CUL-N      |
| PAR-NEW-IN     | 77             |
| PAR-PERSIS     | 2              |
| Derbyshire     | PAR-NEW-IN     |
| E Sussex       | PAR-NEW-IN     |
| Hampshire      | PAR-NEW-IN     |
| SER-NSR        | 1              |
| Leics & Rut    | PAR-NEW-IN     |
| Northants      | PAR-NEW-IN     |
| Notts          | PAR-NEW-IN     |
| Oxon           | PAR-NEW-IN     |
| Warks          | PAR-NEW-IN     |
| PAR-PERSIS     | 2              |
| England - LRA  | Cambs          |
| Cumbria        | PAR-NEW-IN     |
| Gr Manchester  | PAR-NEW-IN     |
| I of Wight     | PAR-NEW-IN     |
| Lancashire     | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 531             | 67619          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 33             |
| PAR-RAPID       | 27             |
| SER-FLEXI       | 15             |
| Ceredigion      | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 8              |
| PAR-RAPID       | 14             |
| SER-FLEXI       | 1              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 8              |
| PAR-RAPID       | 14             |
| Pembrokeshire   | PAR-RAPID      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 10             |
| PAR-PERSIS      | 25             |
| PAR-RAPID       | 42             |
| SER-FLEXI       | 5              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 5              |
| PAR-PERSIS      | 9              |
| PAR-RAPID       | 19             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FLEXI               | 2              |
| S Wales                 | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| SER-FLEXI               | 1              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-RAPID               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 18             |
| Powys                   | PAR-NEW-IN     |
| W Glamorgan             | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-RAPID               | 1              |
| Wales - Low TB          | Gwynedd        |
| NE Wales                | PAR-NEW-IN     |
| Wales                   |                |
| 390                     | 17196          |
| Scotland - Scotland     | Ayrshire       |
| Scotland                |                |
| 3                       | 366            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 3A. Summary Of Ifn Gamma Results By Country And Protocol March 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 181          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 3            |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 131          |
| GB              | Parallel     |
| Serial/Extended | 10           |
| Total           | 315          |
| 181.00          |              |
| 131.00          |              |
| 3.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 9            |
| Total           | 531          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 3            |
| Wales           | Parallel     |
| Serial/Extended | 42           |
| Total           | 390          |
| GB              | Parallel     |
| Serial/Extended | 51           |
| Total           | 924          |
| 531.00          |              |
| 390.00          |              |
| 3.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (March 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
10
283
1.0%
33
11.7%
239
84.5%
14
4.9 %
11
3.9 %
0
Parallel - Herd or Group Slaughter
27
853
2.9%
75
8.8%
691
81.0%
99
11.6 %
88
10.3 %
0
Parallel New Infection
122
14978
50.6%
633
4.2%
13452
89.8%
1090
7.3 %
889
5.9 %
4
0.0 %
Parallel - new infection cull area
54
6914
23.3%
394
5.7%
6089
88.1%
473
6.8 %
377
5.5 %
54
0.8 %
Parallel - Other
11
504
1.7%
56
11.1%
428
84.9%
25
5.0 %
20
4.0 %
0
Parallel Persistent Infection
34
4252
14.4%
241
5.7%
3768
88.6%
302
7.1 %
242
5.7 %
2
0.0 %
Parallel - persistent infection cull area
11
1713
5.8%
90
5.3%
1548
90.4%
87
5.1 %
74
4.3 %
1
0.1 %
Parallel - Rapid Testing Two-times IRs
46
127
0.4%
24
18.9%
94
74.0%
10
7.9 %
9
7.1 %
0
Total
315
29624
100.0%
1546
5.2%
26309
88.8%
2100
7.1 %
1710
5.8 %
61
0.2 %
England - HRA
Parallel New Infection
2
315
1.1%
33
10.5%
247
78.4%
49
15.6 %
35
11.1 %
0
Parallel - new infection cull area
53
6654
22.5%
375
5.6%
5860
88.1%
461
6.9 %
365
5.5 %
54
0.8 %
Parallel - Other
1
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel Persistent Infection
12
2182
7.4%
180
8.2%
1855
85.0%
187
8.6 %
148
6.8 %
0
Parallel - persistent infection cull area
11
1713
5.8%
90
5.3%
1548
90.4%
87
5.1 %
74
4.3 %
1
0.1 %
England - HRA
79
10866
36.7%
678
6.2%
9511
87.5%
785
7.2 %
623
5.7 %
55
0.5 %
England - Edge
Parallel New Infection
94
12140
41.0%
520
4.3%
10924
90.0%
857
7.1 %
695
5.7 %
1
0.0 %
Parallel - new infection cull area
1
260
0.9%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
1
251
0.8%
1
0.4%
238
94.8%
13
5.2 %
12
4.8 %
0
England - Edge
96
12651
42.7%
540
4.3%
11391
90.0%
882
7.0 %
719
5.7 %
1
0.0 %
England - LRA
Parallel New Infection
6
802
2.7%
23
2.9%
706
88.0%
83
10.3 %
71
8.9 %
2
0.2 %
England - LRA
6
802
2.7%
23
2.9%
706
88.0%
83
10.3 %
71
8.9 %
2
0.2 %
Wales - High TB
Extended Flexible IFN-GAMMA test
6
154
0.5%
13
8.4%
131
85.1%
11
7.1 %
10
6.5 %
0
Parallel - Herd or Group Slaughter
26
839
2.8%
75
8.9%
685
81.6%
91
10.8 %
80
9.5 %
0
Parallel New Infection
1
107
0.4%
19
17.8%
79
73.8%
11
10.3 %
9
8.4 %
0
Parallel - Other
7
345
1.2%
35
10.1%
305
88.4%
9
2.6 %
5
1.4 %
0
Parallel Persistent Infection
21
1819
6.1%
60
3.3%
1675
92.1%
102
5.6 %
82
4.5 %
2
0.1 %
Parallel - Rapid Testing Two-times IRs
42
116
0.4%
23
19.8%
84
72.4%
10
8.6 %
9
7.8 %
0
Wales - High TB
103
3380
11.4%
225
6.7%
2959
87.5%
234
6.9 %
195
5.8 %
2
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
4
129
0.4%
20
15.5%
108
83.7%
3
2.3 %
1
0.8 %
0
Parallel - Herd or Group Slaughter
1
14
0.0%
0
0.0 %
6
42.9%
8
57.1 %
8
57.1 %
0
Parallel New Infection
13
1167
3.9%
30
2.6%
1074
92.0%
68
5.8 %
62
5.3 %
1
0.1 %
Parallel - Other
3
157
0.5%
21
13.4%
122
77.7%
15
9.6 %
14
8.9 %
0
Parallel - Rapid Testing Two-times IRs
4
11
0.0%
1
9.1%
10
90.9%
0
0
0
Wales - Intermediate TB
25
1478
5.0%
72
4.9%
1320
89.3%
94
6.4 %
85
5.8 %
1
0.1 %
Wales - Low TB
Parallel New Infection
3
81
0.3%
4
4.9%
77
95.1%
2
2.5 %
0
0
Wales - Low TB
3
81
0.3%
4
4.9%
77
95.1%
2
2.5 %
0
0
Scotland - Scotland
Parallel New Infection
3
366
1.2%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
Scotland - Scotland
3
366
1.2%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
49
1430
1.7%
137
9.6%
1123
78.5%
100
7.0 %
72
5.0 %
98
6.9 %
Parallel - Herd or Group Slaughter
62
2529
3.0%
191
7.6%
2145
84.8%
220
8.7 %
189
7.5 %
5
0.2 %
Parallel New Infection
383
42785
50.2%
1854
4.3%
37466
87.6%
3257
7.6 %
2732
6.4 %
733
1.7 %
Parallel - new infection cull area
116
14727
17.3%
884
6.0%
12731
86.4%
1053
7.2 %
880
6.0 %
232
1.6 %
Parallel - Other
32
2413
2.8%
188
7.8%
2031
84.2%
186
7.7 %
152
6.3 %
42
1.7 %
Parallel Persistent Infection
124
13685
16.1%
940
6.9%
11818
86.4%
989
7.2 %
807
5.9 %
121
0.9 %
Parallel - persistent infection cull area
29
7176
8.4%
431
6.0%
6326
88.2%
494
6.9 %
412
5.7 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
127
420
0.5%
111
26.4%
260
61.9%
39
9.3 %
31
7.4 %
18
4.3 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
Total
924
85181
100.0%
4736
5.6%
73916
86.8%
6338
7.4 %
5275
6.2 %
1256
1.5 %
England - HRA
Extended Flexible IFN-GAMMA test
7
329
0.4%
36
10.9%
241
73.3%
23
7.0 %
7
2.1 %
45
13.7 %
Parallel New Infection
5
791
0.9%
107
13.5%
600
75.9%
104
13.1 %
84
10.6 %
0
Parallel - new infection cull area
115
14467
17.0%
865
6.0%
12502
86.4%
1041
7.2 %
868
6.0 %
232
1.6 %
Parallel - Other
10
806
0.9%
74
9.2%
640
79.4%
59
7.3 %
50
6.2 %
42
5.2 %
Parallel Persistent Infection
33
4392
5.2%
425
9.7%
3566
81.2%
366
8.3 %
299
6.8 %
103
2.3 %
Parallel - persistent infection cull area
29
7176
8.4%
431
6.0%
6326
88.2%
494
6.9 %
412
5.7 %
7
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
305
37078
43.5%
1569
4.2%
32507
87.7%
2789
7.5 %
2334
6.3 %
668
1.8 %
Parallel - new infection cull area
1
260
0.3%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
5
513
0.6%
15
2.9%
474
92.4%
27
5.3 %
24
4.7 %
0
Serial - Suspected NSR Herd
1
7
0.0%
0
0.0 %
7
100.0%
0
0
0
England - LRA
Parallel New Infection
19
1791
2.1%
60
3.4%
1556
86.9%
159
8.9 %
137
7.6 %
38
2.1 %
England
531
67619
79.4%
3601
5.3%
58657
86.7%
5074
7.5 %
4227
6.3 %
1135
1.7 %
Wales - High TB
Extended Flexible IFN-GAMMA test
24
556
0.7%
47
8.5%
469
84.4%
46
8.3 %
40
7.2 %
0
Parallel - Herd or Group Slaughter
58
2503
2.9%
188
7.5%
2132
85.2%
210
8.4 %
179
7.2 %
5
0.2 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Parallel New Infection
6
153
0.2%
24
15.7%
114
74.5%
17
11.1 %
15
9.8 %
0
Parallel - Other
15
1375
1.6%
75
5.5%
1219
88.7%
103
7.5 %
81
5.9 %
0
Parallel Persistent Infection
85
8774
10.3%
500
5.7%
7772
88.6%
596
6.8 %
484
5.5 %
18
0.2 %
Parallel - Rapid Testing Two-times IRs
118
390
0.5%
106
27.2%
236
60.5%
38
9.7 %
30
7.7 %
18
4.6 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
18
545
0.6%
54
9.9%
413
75.8%
31
5.7 %
25
4.6 %
53
9.7 %
Parallel - Herd or Group Slaughter
4
26
0.0%
3
11.5%
13
50.0%
10
38.5 %
10
38.5 %
0
Parallel New Infection
33
2394
2.8%
82
3.4%
2161
90.3%
160
6.7 %
140
5.8 %
11
0.5 %
Parallel - Other
7
232
0.3%
39
16.8%
172
74.1%
24
10.3 %
21
9.1 %
0
Parallel Persistent Infection
1
6
0.0%
0
0.0 %
6
100.0%
0
0
0
Parallel - Rapid Testing Two-times IRs
9
30
0.0%
5
16.7%
24
80.0%
1
3.3 %
1
3.3 %
0
Wales - Low TB
Parallel New Infection
12
212
0.2%
8
3.8%
183
86.3%
8
3.8 %
5
2.4 %
16
7.5 %
Wales
390
17196
20.2%
1131
6.6%
14914
86.7%
1244
7.2 %
1031
6.0 %
121
0.7 %
Scotland - Scotland
Parallel New Infection
3
366
0.4%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
Scotland
3
366
0.4%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | April   |
| May          | 250     |
| June         | 187     |
| July         | 207     |
| August       | 228     |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| Totals (last |         |
| 12 months)   |         |
| 2991         | 280906  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1672
217740
1152
10601
2850
0
0
0
0
788
13
11
27
4
8329
7
75
1
3
Scotland
21
1425
13
92
42
0
0
0
0
22
0
0
0
0
49
0
2
0
0
Wales
1298
61741
655
3316
1793
0
0
0
0
143
5
2
22
4
2135
105
499
2
22
Totals
2991
280906
1820
14009
4685
0
0
0
0
953
18
13
49
8
10513
112
576
3
25
County
%Resampled %Retested
| Ayrshire        | 4.64 %   | 5.46 %   |
|-----------------|----------|----------|
| Berks           | 7.16 %   | 8.18 %   |
| Berkshire       |          |          |
| Bucks           | 6.38 %   | 6.81 %   |
| Cambs           | 100.00 % | 100.00 % |
| Carms           | 6.12 %   | 7.14 %   |
| Ceredigion      | 14.04 %  | 14.62 %  |
| Cheshire        | 5.11 %   | 6.28 %   |
| Corn & Scilly   | 6.47 %   | 8.01 %   |
| Derbyshire      | 10.41 %  | 11.38 %  |
| Devon           | 5.29 %   | 7.16 %   |
| Dorset          | 4.76 %   | 6.28 %   |
| E Sussex        | 27.27 %  | 36.36 %  |
| Gloucestershire | 7.08 %   | 8.85 %   |
| Gloucs          | 5.38 %   | 6.30 %   |
| Gr Manchester   | 23.44 %  | 26.56 %  |
| Gwent           | 10.72 %  | 12.97 %  |
| Gwynedd         | 2.67 %   |          |
| Heref           | 9.39 %   | 11.19 %  |
| I of Wight      | 10.28 %  | 11.78 %  |
| Leics & Rut     | 5.13 %   | 6.25 %   |
| NE Wales        | 4.52 %   | 5.23 %   |
| Northants       | 3.91 %   | 5.55 %   |
| Notts           | 7.28 %   | 10.60 %  |
| N Yorks         | 4.88 %   | 5.69 %   |
|-----------------|----------|----------|
| Oxon            | 5.37 %   | 6.99 %   |
| Pembrokeshire   |          |          |
| Pembs           | 4.95 %   | 6.13 %   |
| Powys           | 4.22 %   | 4.45 %   |
| Somerset excl N | 3.95 %   | 4.81 %   |
| Staffs          | 7.23 %   | 7.93 %   |
| S Wales         | 3.83 %   | 4.26 %   |
| S Yorks         | 0.68 %   | 2.04 %   |
| Warks           | 6.57 %   | 8.36 %   |
| W Glamorgan     |          |          |
| Wiltshire   | 7.78 %   | 9.08 %   |
|-------------|----------|----------|
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23913
1241
5.2%
24319
1241
5.1%
Wales
4862
301
6.2%
4939
301
6.1%
Scotland
366
4
1.1%
366
4
1.1%
GB
29141
1546
5.3%
29624
1546
5.2%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
13258
1013
7.6%
13745
1013
7.4%
Wales
2946
146
5.0%
2991
146
4.9%
Scotland
556
40
7.2%
556
40
7.2%
GB
16760
1199
7.2%
17292
1199
6.9%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
64280
3601
5.6%
67619
3601
5.3%
Wales
16382
1131
6.9%
17196
1131
6.6%
Scotland
366
4
1.1%
366
4
1.1%
GB
81028
4736
5.8%
85181
4736
5.6%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
41557
2620
6.3%
43517
2620
6.0%
Wales
12421
684
5.5%
13062
684
5.2%
Scotland
1165
93
8.0%
1182
93
7.9%
GB
55143
3397
6.2%
57761
3397
5.9%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
Isle of Man
SER-IOM
5
27
1
3.70 %
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Gloucestershire     | PRV-SP        |
| Somerset excl North | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 7                   |               |
| 53                  |               |
| 15                  | 28.30 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|               | Test             |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|---------------|------------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077        | North East Wales |        4 |               154 | 33                   | 21.4 %                            |
| Pembrokeshire | 1                |       32 |                   |                      |                                   |
| South Wales   | 1                |        6 |                 1 | 16.7 %               |                                   |
| Wiltshire     | 1                |       43 |                 2 | 4.7 %                |                                   |
| TC0077        | Sum:             |      235 |                36 | 15.3 %               |                                   |
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |               125 | 4                    | 3.2 %                             |
| North East Wales | 8               |      273 |                40 | 14.7 %               |                                   |
| Pembrokeshire    | 1               |       32 |                   |                      |                                   |
| South Wales      | 1               |        6 |                 1 | 16.7 %               |                                   |
| Wiltshire        | 1               |       43 |                 2 | 4.7 %                |                                   |
| TC0077           | Sum:            |      479 |                47 | 9.8 %                |                                   |