# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 152

# Report Period 1St - 30Th April 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |

## Table 1. Monthly Totals For April 2019

| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| April 2019              |                          |          |
| Submissions             | 269                      | 1193     |
| Samples                 | Total (%)                | 30298    |
| England                 | 23776                    | 78.47 %  |
| Wales                   | 6522                     | 21.53 %  |
| Scotland                | 366                      | 0.32 %   |
| Parallel Tests          | PAR-CUL-N                | 5965     |
| PAR-CUL-P               | 199                      | 0.66 %   |
| PAR-HERD-S              | 412                      | 1.36 %   |
| PAR-NEW-IN              | 15543                    | 51.30 %  |
| PAR-OTHER               | 47                       | 0.16 %   |
| PAR-PERSIS              | 6785                     | 22.39 %  |
| PAR-RAPID               | 70                       | 0.23 %   |
| Total(% of all samples) | 29021                    | 95.79 %  |
| Serial/Extended Tests   | SER-FLEXI                | 1276     |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 1                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 1277                     | 4.21 %   |
| Total                   | 30298                    | 100.00 % |
| Retests                 | Total (% of all samples) | 1897     |
| England                 | 1432                     | 4.73 %   |
| Wales                   | 465                      | 1.53 %   |
| Scotland                | 20                       | 0.02 %   |
| Resamples               | Total (% of all samples) | 1511     |
| England                 | 1139                     | 3.76 %   |
| Wales                   | 372                      | 1.23 %   |
| Scotland                | 17                       | 0.01 %   |
| Rejects                 | Total (% of all samples) | 316      |
| England                 | 264                      | 0.87 %   |
| Wales                   | 52                       | 0.17 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   5965 | 19.7 %   |
|-------------|--------|----------|
| PAR-CUL-P   |    199 | 0.7 %    |
| PAR-HERD-S  |    412 | 1.4 %    |
| PAR-NEW-IN  |  15543 | 51.3 %   |
| PAR-OTHER   |     47 | 0.2 %    |
| PAR-PERSIS  |   6785 | 22.4 %   |
| PAR-RAPID   |     70 | 0.2 %    |
| SER-FLEXI   |   1276 | 4.2 %    |
| SER-NSR     |      1 | 0.0 %    |
| Total       |  30298 | 100.00 % |
submitted
by County

## Table 2A.  Summary By County For April 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-PERSIS      | 4              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 3              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| PAR-NEW-IN      | 1              |
| SER-FLEXI       | 1              |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 1              |
| Heref           | PAR-PERSIS     |
| Shropshire      | PAR-CUL-P      |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 3              |
| Somerset excl N | PAR-CUL-N      |
| PAR-PERSIS      | 3              |
| Staffs          | PAR-PERSIS     |
| Wiltshire       | PAR-CUL-N      |
| England - Edge  | Berks          |
| PAR-PERSIS      | 2              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| PAR-PERSIS      | 1              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| SER-NSR         | 1              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |
| Essex           | PAR-NEW-IN     |
| Gr Manchester   | PAR-NEW-IN     |
| Herts           | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | SER-FLEXI      |
| England         |                |
| 187             | 23776          |
| Wales - High TB | Carms          |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 3              |
| SER-FLEXI       | 2              |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 1              |
| PAR-PERSIS      | 1              |
| PAR-RAPID       | 1              |
| SER-FLEXI       | 1              |
| Gwent           | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-RAPID               | 3              |
| Pembs                   | PAR-HERD-S     |
| PAR-PERSIS              | 15             |
| PAR-RAPID               | 5              |
| SER-FLEXI               | 4              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 1              |
| S Wales                 | PAR-OTHER      |
| PAR-PERSIS              | 1              |
| SER-FLEXI               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 1              |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | PAR-RAPID      |
| Wales                   |                |
| 82                      | 6522           |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019

| Country       | County         |
|---------------|----------------|
| Submission    |                |
| Reasons*      |                |
| No.           |                |
| subs          |                |
| Samples       | Gamma Positive |
| n             | % of total     |
| England - HRA | Avon           |
| SER-FRAUD     | 1              |
| Corn & Scilly | PAR-CUL-N      |
| PAR-CUL-P     | 4              |
| PAR-PERSIS    | 10             |
| SER-FLEXI     | 2              |
| Devon         | PAR-CUL-N      |
| PAR-CUL-P     | 17             |
| PAR-NEW-IN    | 3              |
| PAR-OTHER     | 5              |
| PAR-PERSIS    | 15             |
| SER-FLEXI     | 4              |
| Dorset        | PAR-CUL-N      |
| PAR-CUL-P     | 6              |
| PAR-NEW-IN    | 1              |
| SER-FLEXI     | 1              |
| Gloucs        | PAR-CUL-N      |
| PAR-CUL-P     | 3              |
| Heref         | PAR-CUL-N      |
| PAR-CUL-P     | 1              |
| PAR-PERSIS    | 9              |
| Shropshire    | PAR-CUL-P      |
| PAR-NEW-IN    | 1              |
| PAR-OTHER     | 4              |
| PAR-PERSIS    | 5              |
| SER-FLEXI     | 3              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 3              |
| Staffs          | PAR-PERSIS     |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 1              |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 97             |
| PAR-PERSIS      | 3              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| SER-NSR         | 2              |
| Leics & Rut     | PAR-NEW-IN     |
| Northants       | PAR-NEW-IN     |
| Notts           | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| PAR-PERSIS      | 2              |
| England - LRA   | Cambs          |
| Cumbria         | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Essex           | PAR-NEW-IN     |
| Gr Manchester   | PAR-NEW-IN     |
| Herts           | PAR-NEW-IN     |
| I of Wight      | PAR-NEW-IN     |
| Lancashire      | PAR-NEW-IN     |
| Lincs           | PAR-NEW-IN     |
| N Yorks         | PAR-NEW-IN     |
| SER-FLEXI       | 1              |
| S Yorks         | PAR-NEW-IN     |
| England         |                |
| 718             | 91395          |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 36             |
| PAR-RAPID       | 30             |
| SER-FLEXI       | 17             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 9              |
| PAR-RAPID       | 15             |
| SER-FLEXI       | 2              |
| Gwent           | PAR-HERD-S     |
| PAR-PERSIS      | 8              |
| PAR-RAPID       | 17             |
| Pembrokeshire   | PAR-RAPID      |
| Pembs           | PAR-HERD-S     |
| PAR-OTHER       | 10             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 40             |
| PAR-RAPID               | 47             |
| SER-FLEXI               | 9              |
| Powys                   | PAR-HERD-S     |
| PAR-NEW-IN              | 7              |
| PAR-PERSIS              | 13             |
| PAR-RAPID               | 26             |
| SER-FLEXI               | 3              |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 2              |
| SER-FLEXI               | 2              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 1              |
| Wales - Intermediate TB | Carms          |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 2              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 8              |
| SER-FLEXI               | 19             |
| Powys                   | PAR-NEW-IN     |
| S Glamorgan             | PAR-RAPID      |
| W Glamorgan             | PAR-HERD-S     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 2B.  Summary By County For 2019 Cont.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| PAR-OTHER           | 1              |
| PAR-RAPID           | 1              |
| Wales - Low TB      | Gwynedd        |
| NE Wales            | PAR-NEW-IN     |
| Wales               |                |
| 472                 | 23718          |
| Scotland - Scotland | Ayrshire       |
| Scotland            |                |
| 3                   | 366            |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Par-Herd-S: Potential Herd Slaughter, Par-P-Conf (Par-Persis From 1 April 2017): Persistent Tb Breakdown Otfw, Par-Low-In (Par-New-In From 1 April 2017): Parallel Low Incidence, Par-Rapid: Rapid Testing Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 3A. Summary Of Ifn Gamma Results By Country And Protocol April 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 6            |
| Total           | 187          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 0            |
| Wales           | Parallel     |
| Serial/Extended | 10           |
| Total           | 82           |
| GB              | Parallel     |
| Serial/Extended | 16           |
| Total           | 269          |
| 187.00          |              |
| 82.00           |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 15           |
| Total           | 718          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 3            |
| Wales           | Parallel     |
| Serial/Extended | 52           |
| Total           | 472          |
| GB              | Parallel     |
| Serial/Extended | 67           |
| Total           | 1193 115479  |
| 718.00          |              |
| 472.00          |              |
| 3.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
15
1276
4.2%
49
3.8%
1176
92.2%
61
4.8 %
49
3.8 %
2
0.2 %
Parallel - Herd or Group Slaughter
10
412
1.4%
21
5.1%
362
87.9%
40
9.7 %
29
7.0 %
0
Parallel New Infection
109
15543
51.3%
538
3.5%
14181
91.2%
969
6.2 %
768
4.9 %
56
0.4 %
Parallel - new infection cull area
55
5965
19.7%
277
4.6%
5279
88.5%
358
6.0 %
278
4.7 %
131
2.2 %
Parallel - Other
4
47
0.2%
5
10.6%
40
85.1%
2
4.3 %
2
4.3 %
0
Parallel Persistent Infection
47
6785
22.4%
217
3.2%
6082
89.6%
448
6.6 %
369
5.4 %
119
1.8 %
Parallel - persistent infection cull area
6
199
0.7%
14
7.0%
169
84.9%
17
8.5 %
16
8.0 %
0
Parallel - Rapid Testing Two-times IRs
22
70
0.2%
12
17.1%
50
71.4%
2
2.9 %
0
8
11.4 %
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
Total
269
30298
100.0%
1133
3.7%
27340
90.2%
1897
6.3 %
1511
5.0 %
316
1.0 %
England - HRA
Extended Flexible IFN-GAMMA test
4
639
2.1%
24
3.8%
583
91.2%
40
6.3 %
32
5.0 %
0
Parallel New Infection
1
23
0.1%
1
4.3%
17
73.9%
5
21.7 %
5
21.7 %
0
Parallel - new infection cull area
55
5965
19.7%
277
4.6%
5279
88.5%
358
6.0 %
278
4.7 %
131
2.2 %
Parallel - Other
2
28
0.1%
3
10.7%
23
82.1%
2
7.1 %
2
7.1 %
0
Parallel Persistent Infection
19
2773
9.2%
120
4.3%
2391
86.2%
176
6.3 %
144
5.2 %
118
4.3 %
Parallel - persistent infection cull area
6
199
0.7%
14
7.0%
169
84.9%
17
8.5 %
16
8.0 %
0
England - HRA
87
9627
31.8%
439
4.6%
8462
87.9%
598
6.2 %
477
5.0 %
249
2.6 %
England - Edge
Parallel New Infection
79
11539
38.1%
387
3.4%
10631
92.1%
647
5.6 %
506
4.4 %
15
0.1 %
Parallel Persistent Infection
3
324
1.1%
2
0.6%
317
97.8%
6
1.9 %
5
1.5 %
0
Serial - Suspected NSR Herd
1
1
0.0%
0
0.0 %
1
100.0%
0
0
0
England - Edge
83
11864
39.2%
389
3.3%
10949
92.3%
653
5.5 %
511
4.3 %
15
0.1 %
England - LRA
Extended Flexible IFN-GAMMA test
1
49
0.2%
1
2.0%
47
95.9%
3
6.1 %
1
2.0 %
0
Parallel New Infection
16
2236
7.4%
89
4.0%
1997
89.3%
178
8.0 %
150
6.7 %
0
England - LRA
17
2285
7.5%
90
3.9%
2044
89.5%
181
7.9 %
151
6.6 %
0
Wales - High TB
Extended Flexible IFN-GAMMA test
9
571
1.9%
24
4.2%
532
93.2%
15
2.6 %
13
2.3 %
2
0.4 %
Parallel - Herd or Group Slaughter
10
412
1.4%
21
5.1%
362
87.9%
40
9.7 %
29
7.0 %
0
Parallel New Infection
3
288
1.0%
13
4.5%
265
92.0%
10
3.5 %
10
3.5 %
0
Parallel - Other
1
11
0.0%
0
0.0 %
11
100.0%
0
0
0
Parallel Persistent Infection
24
3573
11.8%
83
2.3%
3274
91.6%
263
7.4 %
217
6.1 %
1
0.0 %
Parallel - Rapid Testing Two-times IRs
19
57
0.2%
8
14.0%
41
71.9%
2
3.5 %
0
8
14.0 %
Wales - High TB
66
4912
16.2%
149
3.0%
4485
91.3%
330
6.7 %
269
5.5 %
11
0.2 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
1
17
0.1%
0
0.0 %
14
82.4%
3
17.6 %
3
17.6 %
0
Parallel New Infection
10
1457
4.8%
48
3.3%
1271
87.2%
129
8.9 %
97
6.7 %
41
2.8 %
Parallel - Other
1
8
0.0%
2
25.0%
6
75.0%
0
0
0
Parallel Persistent Infection
1
115
0.4%
12
10.4%
100
87.0%
3
2.6 %
3
2.6 %
0
Parallel - Rapid Testing Two-times IRs
3
13
0.0%
4
30.8%
9
69.2%
0
0
0
Wales - Intermediate TB
16
1610
5.3%
66
4.1%
1400
87.0%
135
8.4 %
103
6.4 %
41
2.5 %
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
64
2706
2.3%
186
6.9%
2299
85.0%
161
5.9 %
121
4.5 %
100
3.7 %
Parallel - Herd or Group Slaughter
72
2941
2.5%
212
7.2%
2507
85.2%
260
8.8 %
218
7.4 %
5
0.2 %
Parallel New Infection
492
58328
50.5%
2392
4.1%
51647
88.5%
4226
7.2 %
3500
6.0 %
789
1.4 %
Parallel - new infection cull area
171
20692
17.9%
1161
5.6%
18010
87.0%
1411
6.8 %
1158
5.6 %
363
1.8 %
Parallel - Other
36
2460
2.1%
193
7.8%
2071
84.2%
188
7.6 %
154
6.3 %
42
1.7 %
Parallel Persistent Infection
171
20470
17.7%
1157
5.7%
17900
87.4%
1437
7.0 %
1176
5.7 %
240
1.2 %
Parallel - persistent infection cull area
35
7375
6.4%
445
6.0%
6495
88.1%
511
6.9 %
428
5.8 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
149
490
0.4%
123
25.1%
310
63.3%
41
8.4 %
31
6.3 %
26
5.3 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
1193 115479
100.0%
5869
5.1%
101256
87.7%
8235
7.1 %
6786
5.9 %
1572
1.4 %
England - HRA
Extended Flexible IFN-GAMMA test
11
968
0.8%
60
6.2%
824
85.1%
63
6.5 %
39
4.0 %
45
4.6 %
Parallel New Infection
6
814
0.7%
108
13.3%
617
75.8%
109
13.4 %
89
10.9 %
0
Parallel - new infection cull area
170
20432
17.7%
1142
5.6%
17781
87.0%
1399
6.8 %
1146
5.6 %
363
1.8 %
Parallel - Other
12
834
0.7%
77
9.2%
663
79.5%
61
7.3 %
52
6.2 %
42
5.0 %
Parallel Persistent Infection
52
7165
6.2%
545
7.6%
5957
83.1%
542
7.6 %
443
6.2 %
221
3.1 %
Parallel - persistent infection cull area
35
7375
6.4%
445
6.0%
6495
88.1%
511
6.9 %
428
5.8 %
7
0.1 %
Serial - Anomalous Reaction
1
9
0.0%
0
0.0 %
9
100.0%
0
0
0
England - Edge
Parallel New Infection
384
48617
42.1%
1956
4.0%
43138
88.7%
3436
7.1 %
2840
5.8 %
683
1.4 %
Parallel - new infection cull area
1
260
0.2%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
8
837
0.7%
17
2.0%
791
94.5%
33
3.9 %
29
3.5 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
1
49
0.0%
1
2.0%
47
95.9%
3
6.1 %
1
2.0 %
0
Parallel New Infection
35
4027
3.5%
149
3.7%
3553
88.2%
337
8.4 %
287
7.1 %
38
0.9 %
England
718
91395
79.1%
4519
4.9%
80112
87.7%
6506
7.1 %
5366
5.9 %
1399
1.5 %
Wales - High TB
Extended Flexible IFN-GAMMA test
33
1127
1.0%
71
6.3%
1001
88.8%
61
5.4 %
53
4.7 %
2
0.2 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

Parallel - Herd or Group Slaughter
68
2915
2.5%
209
7.2%
2494
85.6%
250
8.6 %
208
7.1 %
5
0.2 %
Parallel New Infection
9
441
0.4%
37
8.4%
379
85.9%
27
6.1 %
25
5.7 %
0
Parallel - Other
16
1386
1.2%
75
5.4%
1230
88.7%
103
7.4 %
81
5.8 %
0
Parallel Persistent Infection
109
12347
10.7%
583
4.7%
11046
89.5%
859
7.0 %
701
5.7 %
19
0.2 %
Parallel - Rapid Testing Two-times IRs
137
447
0.4%
114
25.5%
277
62.0%
40
8.9 %
30
6.7 %
26
5.8 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
19
562
0.5%
54
9.6%
427
76.0%
34
6.0 %
28
5.0 %
53
9.4 %
Parallel - Herd or Group Slaughter
4
26
0.0%
3
11.5%
13
50.0%
10
38.5 %
10
38.5 %
0
Parallel New Infection
43
3851
3.3%
130
3.4%
3432
89.1%
289
7.5 %
237
6.2 %
52
1.4 %
Parallel - Other
8
240
0.2%
41
17.1%
178
74.2%
24
10.0 %
21
8.8 %
0
Parallel Persistent Infection
2
121
0.1%
12
9.9%
106
87.6%
3
2.5 %
3
2.5 %
0
Parallel - Rapid Testing Two-times IRs
12
43
0.0%
9
20.9%
33
76.7%
1
2.3 %
1
2.3 %
0
Wales - Low TB
Parallel New Infection
12
212
0.2%
8
3.8%
183
86.3%
8
3.8 %
5
2.4 %
16
7.5 %
Wales
472
23718
20.5%
1346
5.7%
20799
87.7%
1709
7.2 %
1403
5.9 %
173
0.7 %
Scotland - Scotland
Parallel New Infection
3
366
0.3%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
Scotland
3
366
0.3%
4
1.1%
345
94.3%
20
5.5 %
17
4.6 %
0
| Year         | Month   |
|--------------|---------|
| Herds        |         |
| sampled      |         |
| Samples      |         |
| tested       |         |
| IFNg+        |         |
| samples      |         |
| Wrong        |         |
| Eartags      |         |
| VL No        |         |
| Cult         |         |
| Herds        |         |
| with         |         |
| positives    |         |
| No PM        |         |
| No           |         |
| Cult         |         |
| No PM        |         |
| Cult         |         |
| Pend         |         |
| No PM        |         |
| Cult         |         |
| Neg          |         |
| No PM        |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Neg          |         |
| VL           |         |
| Cult         |         |
| Pend         |         |
| VL           |         |
| Cult         |         |
| Mb           |         |
| VL           |         |
| Cult         |         |
| Other        |         |
| NVL          |         |
| No           |         |
| Cult         |         |
| NVL          |         |
| Cult         |         |
| Pend         |         |
| NVL          |         |
| Cult         |         |
| Neg          |         |
| NVL          |         |
| Cult         |         |
| Mb           |         |
| NVL          |         |
| Cult         |         |
| Other        |         |
| No           |         |
| PM           |         |
| Cult         |         |
| Other        |         |
| 2018         | May     |
| June         | 187     |
| July         | 207     |
| August       | 228     |
| September    | 216     |
| October      | 274     |
| November     | 282     |
| December     | 228     |
| 2019         | January |
| February     | 320     |
| March        | 315     |
| April        | 269     |
| Totals (last |         |
| 12 months)   |         |
| 3065         | 290687  |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1757
227265
1198
10769
549
0
0
0
0
815
12
7
35
6
8784
4
80
1
3
Scotland
17
1354
11
51
0
0
0
0
0
0
0
1
0
0
47
3
0
0
0
Wales
1291
62068
655
3369
92
0
0
0
0
161
5
1
22
5
2284
137
479
2
31
Totals
3065
290687
1864
14189
641
0
0
0
0
976
17
9
57
11
11115
144
559
3
34

*Includes all animals with IFNg test negative, resample and reject outcomes

|               | County   | %Resampled %Retested   |
|---------------|----------|------------------------|
| Avon          | 2.22 %   | 2.84 %                 |
| Berks         | 5.32 %   | 6.38 %                 |
| Bucks         | 7.06 %   | 5.37 %                 |
| Cambs         | 7.27 %   | 10.91 %                |
| Carms         | 4.10 %   | 5.51 %                 |
| Ceredigion    | 2.70 %   | 3.70 %                 |
| Cheshire      | 3.63 %   | 5.05 %                 |
| Corn & Scilly | 4.66 %   | 6.48 %                 |
| Cumbria       | 12.64 %  | 14.08 %                |
| Derbyshire    | 7.03 %   | 7.67 %                 |
| Devon         | 4.58 %   | 5.89 %                 |
| Dorset        | 5.06 %   | 6.64 %                 |
| Essex         | 2.17 %   | 2.17 %                 |
| E Sussex      | 3.18 %   | 3.18 %                 |
| Gloucs        | 9.06 %   | 10.24 %                |
| Gr Manchester | 40.00 %  | 53.33 %                |
| Gwent         | 9.80 %   | 9.80 %                 |
| Hampshire     | 5.45 %   | 6.52 %                 |
| Heref         | 6.86 %   | 8.52 %                 |
| Herts         | 6.68 %   | 8.04 %                 |
| Lancashire    | 3.57 %   | 4.76 %                 |
| Leics & Rut   | 3.69 %   | 5.13 %                 |
| Lincs         | 3.95 %   | 4.68 %                 |
| NE Wales   | 6.96 %   | 9.58 %   |
|------------|----------|----------|
| Northants       | 5.99 %   | 7.89 %   |
|-----------------|----------|----------|
| Notts           | 9.09 %   | 9.09 %   |
| N Yorks         | 2.04 %   | 6.12 %   |
| Oxon            | 4.99 %   | 5.91 %   |
| Pembs           | 6.46 %   | 7.70 %   |
| Powys           | 6.42 %   | 7.53 %   |
| S Glamorgan     |          |          |
| Shropshire      | 5.60 %   | 6.72 %   |
| Somerset excl N | 4.49 %   | 5.49 %   |
| Staffs          | 25.81 %  | 29.03 %  |
| S Wales         | 5.00 %   | 5.00 %   |
| Warks     | 4.34 %   | 5.67 %   |
|-----------|----------|----------|
| Wiltshire | 4.77 %   | 5.42 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
23352
916
3.9%
23776
918
3.9%
Wales
6513
215
3.3%
6522
215
3.3%
GB
29865
1131
3.8%
30298
1133
3.7%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
14120
750
5.3%
14251
750
5.3%
Wales
5538
162
2.9%
6195
162
2.6%
Scotland
71
41
57.7%
71
41
57.7%
GB
19729
953
4.8%
20517
953
4.6%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
86644
4517
5.2%
91395
4519
4.9%
Wales
22256
1346
6.0%
23718
1346
5.7%
Scotland
366
4
1.1%
366
4
1.1%
GB
109266
5867
5.4%
115479
5869
5.1%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
53944
3370
6.2%
57768
3370
5.8%
Wales
17758
846
4.8%
19257
846
4.4%
Scotland
1167
134
11.5%
1253
134
10.7%
GB
72868
4350
6.0%
78278
4350
5.6%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Total
0
0
0
Isle of Man
SER-IOM
1
1
0
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Gloucestershire     | PRV-SP        |
| Somerset excl North | PRV-SE        |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 7                   |               |
| 53                  |               |
| 15                  | 28.30 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|               | Test             |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|---------------|------------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077        | North East Wales |        3 |               110 | 23                   | 20.9%                             |
| Pembrokeshire | 1                |       32 |                 1 | 3.1%                 |                                   |
| South Wales   | 1                |      112 |                12 | 10.7%                |                                   |
| TC0077        | Sum:             |      254 |                36 | 14.2 %               |                                   |
|                  | Test            |   County |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire |        2 |               125 | 4                    | 3.2%                              |
| North East Wales | 11              |      383 |                63 | 16.4%                |                                   |
| Pembrokeshire    | 2               |       64 |                 1 | 1.6%                 |                                   |
| South Wales      | 2               |      118 |                13 | 11.0%                |                                   |
| TC0077           | Sum:            |      690 |                81 | 11.7 %               |                                   |