# Monthly Report: Surveillance Project Sb4008 Ifng Tests For Bovine Tuberculosis (Tc0651 And Tc0751)

Number 156

# Report Period 1St - 31St August 2019

## Operational Notes On Terminology, Definitions, Re-Test And Re-Samples Test Outcomes:

- "Samples" refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing.  It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested.  However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S). - "Submissions" refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission).  It is generally assumed that the number of submissions and the number of herds are analogous. - Retests are samples where the first ELISA assay fails and the same sample is  retested on a new ELISA plate. Each sample can be retested only once.  A retest is not a reportable test outcome. - Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample.  This is a reportable test outcome. - Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5. - POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample.  A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response. - NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN-  responses.  A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g.

## Test Criteria

| Submission Reason                                   | Explanation          |
|-----------------------------------------------------|----------------------|
| Possible Herd  Slaughter                            |                      |
| (PAR-HERD-S)                                        |                      |
| Parallel interferon-gamma blood testing of skin     |                      |
| test negative cattle to inform whole or partial     |                      |
| herd slaughters decisions                           |                      |
| Parallel blood testing of skin test-negative cattle |                      |
| in persistently infected herds that have failed to  |                      |
| resolve by repeated short-interval skin testing     |                      |
| and fulfill a minimum of biosecurity standards.     |                      |
| Persistent TB breakdowns                            |                      |
| (OTF status withdrawn)                              |                      |
| (PAR-P-CONF) N.B.                                   |                      |
| PAR-PERSIS from 1st April                           |                      |
| 2017                                                |                      |
| Parallel - Low Incidence                            |                      |
| (PAR-LOW-IN) N.B.                                   |                      |
| PAR-NEW-IN from 1st April                           |                      |
| 2017                                                |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new herd breakdown  as soon as possible after       |                      |
| confirmation of TB.                                 |                      |
| Rapid Testing of twice IR's                         |                      |
| (PAR-RAPID)                                         |                      |
| Parallel blood testing of two-times IRs identified  |                      |
| under the severe interpretation of the skin test    |                      |
| used in Wales.                                      |                      |
| PAR-CUL-N                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| new breakdown as soon as possible after             |                      |
| confirmation of TB where herd is situated in        |                      |
| badger control areas of England that have           |                      |
| completed at least 2 effective culls.               |                      |
| PAR-CUL-P                                           |                      |
| Parallel blood testing to maximise the              |                      |
| probability of removing all infected cattle in a    |                      |
| persistent confirmed breakdown where herd is        |                      |
| situated within badger control areas of England     |                      |
| that have completed at least 2 effective culls      |                      |
| Parallel Other                                      |                      |
| (PAR-OTHER)                                         |                      |
| Other parallel blood testing not covered in any     |                      |
| of the other scenarios                              |                      |
| NO (APHA discretion)                                |                      |
| 'NSR' Herds (SER-NSR)                               |                      |
| Modified serial blood testing of individual skin    |                      |
| test reactors and/or IRs in unconfirmed TB          |                      |
| breakdown herds to clarify their infection status   |                      |
| where there is evidence of non-specific             |                      |
| sensitisation to bovine tuberculin (the             |                      |
| "non-specific reactor" procedure)                   |                      |
| Suspected Fraud                                     |                      |
| (SER-FRAUD)                                         |                      |
| Modified serial blood test of suspected             |                      |
| fraudulent reactors to the skin test (animals with  |                      |
| abnormal skin swellings), in confirmed or           |                      |
| unconfirmed TB incidents.                           |                      |
| Serial Other                                        |                      |
| (SER-OTHER)                                         |                      |
| Serial test - other reasons                         | NO (APHA Discretion) |
| SER-FLEXI                                           |                      |
| Extended blood test to provide flexible test        |                      |
| readout in a confirmed M. bovis-infected herd       |                      |
| where Johne's (M. a. paratuberculosis) infection    |                      |
| or vaccination is suspected to be interferring      |                      |
| with M. bovis infection detection                   |                      |
| YES, if APHA are                                    |                      |
| contemplating a herd                                |                      |
| slaughter                                           |                      |
| NO (APHA discretion)                                |                      |
| YES in Area of Low                                  |                      |
| Incidence                                           |                      |
| YES (Wales only)                                    |                      |
| YES (in eligible badger                             |                      |
| control areas of England)                           |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion)                                |                      |
| NO (APHA discretion to                              |                      |
| firm up or rule out any                             |                      |
| suspicion of fraud and                              |                      |
| support any                                         |                      |
| investigations)                                     |                      |
| NO (APHA discretion)                                |                      |

## Table 1. Monthly Totals For August 2019

| %                       | Total 2019               | %        |
|-------------------------|--------------------------|----------|
| Num                     |                          |          |
| samples                 |                          |          |
| August 2019             |                          |          |
| Submissions             | 262                      | 2422     |
| Samples                 | Total (%)                | 21887    |
| England                 | 18297                    | 83.60 %  |
| Wales                   | 3236                     | 14.79 %  |
| Scotland                | 354                      | 1.62 %   |
| Parallel Tests          | PAR-CUL-N                | 4947     |
| PAR-CUL-P               | 2479                     | 11.33 %  |
| PAR-HERD-S              | 358                      | 1.64 %   |
| PAR-NEW-IN              | 8098                     | 37.00 %  |
| PAR-OTHER               | 226                      | 1.03 %   |
| PAR-PERSIS              | 5286                     | 24.15 %  |
| PAR-RAPID               | 77                       | 0.35 %   |
| Total(% of all samples) | 21471                    | 98.10 %  |
| Serial/Extended Tests   | SER-FLEXI                | 416      |
| SER-FRAUD               | 0                        | 0.00 %   |
| SER-NSR                 | 0                        | 0.00 %   |
| SER-OTHER               | 0                        | 0.00 %   |
| Total(% of all samples) | 416                      | 1.90 %   |
| Total                   | 21887                    | 100.00 % |
| Retests                 | Total (% of all samples) | 2044     |
| England                 | 1837                     | 8.39 %   |
| Wales                   | 195                      | 0.89 %   |
| Scotland                | 12                       | 0.05 %   |
| Resamples               | Total (% of all samples) | 1513     |
| England                 | 1393                     | 6.36 %   |
| Wales                   | 111                      | 0.51 %   |
| Scotland                | 9                        | 0.04 %   |
| Rejects                 | Total (% of all samples) | 96       |
| England                 | 93                       | 0.42 %   |
| Wales                   | 3                        | 0.01 %   |
| Scotland                | 0                        | 0.00 %   |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

| PAR-CUL-N   |   4947 | 22.6 %   |
|-------------|--------|----------|
| PAR-CUL-P   |   2479 | 11.3 %   |
| PAR-HERD-S  |    358 | 1.6 %    |
| PAR-NEW-IN  |   8098 | 37.0 %   |
| PAR-OTHER   |    226 | 1.0 %    |
| PAR-PERSIS  |   5286 | 24.2 %   |
| PAR-RAPID   |     77 | 0.4 %    |
| SER-FLEXI   |    416 | 1.9 %    |
| Total       |  21887 | 100.00 % |
Breakdown
of Samples
submitted
by County

Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2A.  Summary By County For August 2019

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-NEW-IN      | 2              |
| PAR-PERSIS      | 5              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 3              |
| Gloucestershire | PAR-NEW-IN     |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 2              |
| Heref           | PAR-CUL-N      |
| PAR-PERSIS      | 2              |
| Shropshire      | PAR-PERSIS     |
| Somerset excl N | PAR-CUL-N      |
| Staffs          | PAR-OTHER      |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 2              |
| PAR-PERSIS      | 2              |
| England - Edge  | Berks          |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-NEW-IN     |
| Derbyshire      | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |
| Leics & Rut     | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Northants       | PAR-NEW-IN     |
| Oxon            | PAR-NEW-IN     |
| Warks           | PAR-NEW-IN     |
| England - LRA   | Cumbria        |
| Gr London       | PAR-NEW-IN     |
| Northumb        | PAR-NEW-IN     |
| PAR-PERSIS      | 3              |
| N Yorks         | PAR-PERSIS     |
| W Sussex        | PAR-NEW-IN     |
| England         |                |
| 143             | 18297          |
| Wales - High TB | Carms          |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 7              |
| PAR-RAPID       | 6              |
| SER-FLEXI       | 7              |
| Ceredigion      | PAR-OTHER      |
| PAR-PERSIS      | 3              |
| PAR-RAPID       | 2              |
| SER-FLEXI       | 2              |
| Gwent           | PAR-PERSIS     |
| PAR-RAPID       | 2              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |
| PAR-PERSIS      | 16             |
| PAR-RAPID       | 7              |
| SER-FLEXI       | 7              |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 6              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| PAR-PERSIS              | 2              |
| PAR-RAPID               | 7              |
| SER-FLEXI               | 2              |
| Wales - Intermediate TB | Carms          |
| Ceredigion              | PAR-RAPID      |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 1              |
| SER-FLEXI               | 5              |
| W Glamorgan             | PAR-OTHER      |
| PAR-RAPID               | 1              |
| Wales - Low TB          | NE Wales       |
| PAR-OTHER               | 2              |
| SER-FLEXI               | 1              |
| Wales                   |                |
| 117                     | 3236           |
| Scotland - Scotland     | Dumfries & G   |
| Kirkcudbright           | PAR-PERSIS     |
| Scotland                |                |
| 2                       | 354            |
| Grand Total             | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| England - HRA   | Avon           |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 9              |
| SER-FRAUD       | 2              |
| Corn & Scilly   | PAR-CUL-N      |
| PAR-CUL-P       | 4              |
| PAR-NEW-IN      | 4              |
| PAR-PERSIS      | 29             |
| SER-FLEXI       | 2              |
| Devon           | PAR-CUL-N      |
| PAR-CUL-P       | 22             |
| PAR-NEW-IN      | 4              |
| PAR-OTHER       | 7              |
| PAR-PERSIS      | 19             |
| SER-FLEXI       | 8              |
| Dorset          | PAR-CUL-N      |
| PAR-CUL-P       | 13             |
| PAR-NEW-IN      | 2              |
| SER-FLEXI       | 4              |
| Gloucestershire | PAR-NEW-IN     |
| Gloucs          | PAR-CUL-N      |
| PAR-CUL-P       | 8              |
| PAR-HERD-S      | 4              |
| SER-FLEXI       | 2              |
| Heref           | PAR-CUL-N      |
| PAR-CUL-P       | 2              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 25             |
| Shropshire      | PAR-CUL-P      |
| PAR-NEW-IN      | 1              |
| PAR-OTHER       | 4              |
| PAR-PERSIS      | 10             |
| SER-FLEXI       | 5              |
| Somerset excl N | PAR-CUL-N      |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 13             |
| Staffs          | PAR-OTHER      |
| PAR-PERSIS      | 7              |
| Wiltshire       | PAR-CUL-N      |
| PAR-CUL-P       | 15             |
| PAR-NEW-IN      | 6              |
| PAR-OTHER       | 2              |
| PAR-PERSIS      | 6              |
| SER-FLEXI       | 1              |
| England - Edge  | Berks          |
| PAR-PERSIS      | 3              |
| Bucks           | PAR-NEW-IN     |
| Cheshire        | PAR-CUL-N      |
| PAR-NEW-IN      | 160            |
| PAR-PERSIS      | 5              |
| Derbyshire      | PAR-NEW-IN     |
| E Sussex        | PAR-NEW-IN     |
| Hampshire       | PAR-NEW-IN     |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country            | County         |
|--------------------|----------------|
| Submission         |                |
| Reasons*           |                |
| No.                |                |
| subs               |                |
| Samples            | Gamma Positive |
| n                  | % of total     |
| SER-NSR            | 2              |
| Leicestershire and |                |
| Rutland            |                |
| PAR-NEW-IN         | 1              |
| Leics & Rut        | PAR-NEW-IN     |
| Northants          | PAR-NEW-IN     |
| Notts              | PAR-NEW-IN     |
| Oxon               | PAR-NEW-IN     |
| Warks              | PAR-NEW-IN     |
| PAR-PERSIS         | 6              |
| England - LRA      | Cambs          |
| Cumbria            | PAR-NEW-IN     |
| Essex              | PAR-NEW-IN     |
| Gr London          | PAR-NEW-IN     |
| Gr Manchester      | PAR-NEW-IN     |
| Herts              | PAR-NEW-IN     |
| I of Wight         | PAR-NEW-IN     |
| Kent               | PAR-NEW-IN     |
| Lancashire         | PAR-NEW-IN     |
| Lincs              | PAR-NEW-IN     |
| Northumb           | PAR-NEW-IN     |
| PAR-PERSIS         | 3              |
| N Yorks            | PAR-NEW-IN     |
| PAR-PERSIS         | 2              |
| SER-FLEXI          | 2              |
| S Yorks            | PAR-NEW-IN     |
| W Sussex           | PAR-NEW-IN     |
| W Yorks            | PAR-HERD-S     |
| England            |                |
| 1431               | 179993         |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs,  PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other

## Table 2B.  Summary By County For 2019 Cont.

| Country         | County         |
|-----------------|----------------|
| Submission      |                |
| Reasons*        |                |
| No.             |                |
| subs            |                |
| Samples         | Gamma Positive |
| n               | % of total     |
| Wales - High TB | Carms          |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 13             |
| PAR-PERSIS      | 59             |
| PAR-RAPID       | 59             |
| SER-FLEXI       | 35             |
| Ceredigion      | PAR-HERD-S     |
| PAR-NEW-IN      | 2              |
| PAR-OTHER       | 3              |
| PAR-PERSIS      | 22             |
| PAR-RAPID       | 35             |
| SER-FLEXI       | 5              |
| Gwent           | PAR-HERD-S     |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 16             |
| PAR-RAPID       | 24             |
| SER-FLEXI       | 1              |
| Pembs           | PAR-HERD-S     |
| PAR-NEW-IN      | 3              |
| PAR-OTHER       | 14             |
| PAR-PERSIS      | 105            |
| PAR-RAPID       | 92             |
| SER-FLEXI       | 38             |
| Powys           | PAR-HERD-S     |
| PAR-NEW-IN      | 21             |
| PAR-OTHER       | 1              |
| PAR-PERSIS      | 29             |
| PAR-RAPID       | 51             |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country                 | County         |
|-------------------------|----------------|
| Submission              |                |
| Reasons*                |                |
| No.                     |                |
| subs                    |                |
| Samples                 | Gamma Positive |
| n                       | % of total     |
| SER-FLEXI               | 11             |
| S Wales                 | PAR-HERD-S     |
| PAR-OTHER               | 1              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 1              |
| SER-FLEXI               | 3              |
| W Glamorgan             | PAR-HERD-S     |
| PAR-PERSIS              | 1              |
| PAR-RAPID               | 3              |
| Wales - Intermediate TB | Carms          |
| PAR-NEW-IN              | 1              |
| PAR-OTHER               | 6              |
| PAR-PERSIS              | 7              |
| PAR-RAPID               | 10             |
| SER-FLEXI               | 1              |
| Ceredigion              | PAR-HERD-S     |
| PAR-OTHER               | 2              |
| PAR-PERSIS              | 4              |
| PAR-RAPID               | 3              |
| SER-FLEXI               | 2              |
| NE Wales                | PAR-NEW-IN     |
| PAR-OTHER               | 4              |
| PAR-PERSIS              | 3              |
| PAR-RAPID               | 12             |
| SER-FLEXI               | 31             |
| Powys                   | PAR-NEW-IN     |
| PAR-RAPID               | 1              |
| S Glamorgan             | PAR-RAPID      |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

| Country             | County         |
|---------------------|----------------|
| Submission          |                |
| Reasons*            |                |
| No.                 |                |
| subs                |                |
| Samples             | Gamma Positive |
| n                   | % of total     |
| SER-FLEXI           | 3              |
| S Wales             | PAR-RAPID      |
| W Glamorgan         | PAR-HERD-S     |
| PAR-OTHER           | 2              |
| PAR-RAPID           | 3              |
| Wales - Low TB      | Gwynedd        |
| PAR-RAPID           | 1              |
| NE Wales            | PAR-NEW-IN     |
| PAR-OTHER           | 2              |
| PAR-PERSIS          | 2              |
| PAR-RAPID           | 1              |
| SER-FLEXI           | 2              |
| Wales               |                |
| 976                 | 42378          |
| Scotland - Scotland | Argyll & Bute  |
| Ayrshire            | PAR-NEW-IN     |
| Dumfries & G        | PAR-NEW-IN     |
| Kirkcudbright       | PAR-NEW-IN     |
| PAR-PERSIS          | 1              |
| Wigtown             | PAR-NEW-IN     |
| Scotland            |                |
| 15                  | 2210           |
| Grand Total         | Sum:           |

*For test criteria please refer to Operational notes.

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Of Twice Irs,  Par-Cul-N: New Infection In Cull Area, Par-Cul-P: Persistent Infection In Cull Area, Par-Other: Parallel Other, Ser-Flexi: Flexible Extended Test In Confirmed Herds With Concurrent Johne'S Infection/Vaccination, Ser-Nsr: Serial Non-Specific Reactor, Ser-Fraud: Serial Potential Fraud, Ser-Other: Serial Other Table 3A. Summary Of Ifn Gamma Results By Country And Protocol August 2019

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 0            |
| Total           | 143          |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 2            |
| Wales           | Parallel     |
| Serial/Extended | 24           |
| Total           | 117          |
| GB              | Parallel     |
| Serial/Extended | 24           |
| Total           | 262          |
| 143.00          |              |
| 117.00          |              |
| 2.00            |              |

* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 3B. Summary Of Ifn Gamma Results By Country And Protocol (Total 2019)

| Country         | Protocol     |
|-----------------|--------------|
| No              |              |
| submissions     | Samples      |
| n               | % of total n |
| England         | Parallel     |
| Serial/Extended | 28           |
| Total           | 1431 179993  |
| Scotland        | Parallel     |
| Serial/Extended | 0            |
| Total           | 15           |
| Wales           | Parallel     |
| Serial/Extended | 132          |
| Total           | 976          |
| GB              | Parallel     |
| Serial/Extended | 160          |
| Total           | 2422 224581  |

#######
976.00
15.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample.  Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported.  Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

## Table 4A.  Summaryof Ifn Gamma Results By Submission Reason  (August 2019)

Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
24
416
1.9%
28
6.7%
368
88.5%
25
6.0 %
19
4.6 %
1
0.2 %
Parallel - Herd or Group Slaughter
10
358
1.6%
24
6.7%
326
91.1%
28
7.8 %
8
2.2 %
0
Parallel New Infection
81
8098
37.0%
190
2.3%
7282
89.9%
863
10.7 %
627
7.7 %
1
0.0 %
Parallel - new infection cull area
47
4947
22.6%
201
4.1%
4366
88.3%
478
9.7 %
380
7.7 %
0
Parallel - Other
11
226
1.0%
9
4.0%
198
87.6%
24
10.6 %
19
8.4 %
0
Parallel Persistent Infection
49
5286
24.2%
136
2.6%
4752
89.9%
392
7.4 %
305
5.8 %
93
1.8 %
Parallel - persistent infection cull area
11
2479
11.3%
119
4.8%
2207
89.0%
230
9.3 %
153
6.2 %
0
Parallel - Rapid Testing Two-times IRs
29
77
0.4%
17
22.1%
57
74.0%
4
5.2 %
2
2.6 %
1
1.3 %
Total
262
21887
100.0%
724
3.3%
19556
89.3%
2044
9.3 %
1513
6.9 %
96
0.4 %
England - HRA
Parallel New Infection
5
361
1.6%
8
2.2%
304
84.2%
62
17.2 %
49
13.6 %
0
Parallel - new infection cull area
47
4947
22.6%
201
4.1%
4366
88.3%
478
9.7 %
380
7.7 %
0
Parallel - Other
3
188
0.9%
3
1.6%
169
89.9%
18
9.6 %
16
8.5 %
0
Parallel Persistent Infection
12
2376
10.9%
32
1.3%
2190
92.2%
199
8.4 %
154
6.5 %
0
Parallel - persistent infection cull area
11
2479
11.3%
119
4.8%
2207
89.0%
230
9.3 %
153
6.2 %
0
England - HRA
78
10351
47.3%
363
3.5%
9236
89.2%
987
9.5 %
752
7.3 %
0
England - Edge
Parallel New Infection
55
6907
31.6%
145
2.1%
6287
91.0%
663
9.6 %
477
6.9 %
0
England - Edge
55
6907
31.6%
145
2.1%
6287
91.0%
663
9.6 %
477
6.9 %
0
England - LRA
Parallel New Infection
5
303
1.4%
0
0.0 %
218
71.9%
98
32.3 %
85
28.1 %
0
Parallel Persistent Infection
5
736
3.4%
3
0.4%
561
76.2%
89
12.1 %
79
10.7 %
93
12.6 %
England - LRA
10
1039
4.7%
3
0.3%
779
75.0%
187
18.0 %
164
15.8 %
93
9.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
18
315
1.4%
23
7.3%
277
87.9%
18
5.7 %
14
4.4 %
1
0.3 %
Parallel - Herd or Group Slaughter
10
358
1.6%
24
6.7%
326
91.1%
28
7.8 %
8
2.2 %
0
Parallel New Infection
9
212
1.0%
13
6.1%
190
89.6%
14
6.6 %
8
3.8 %
1
0.5 %
Parallel - Other
3
15
0.1%
5
33.3%
10
66.7%
2
13.3 %
0
0
Parallel Persistent Infection
30
1602
7.3%
88
5.5%
1455
90.8%
81
5.1 %
59
3.7 %
0
Parallel - Rapid Testing Two-times IRs
24
69
0.3%
14
20.3%
52
75.4%
4
5.8 %
2
2.9 %
1
1.4 %
Wales - High TB
94
2571
11.7%
167
6.5%
2310
89.8%
147
5.7 %
91
3.5 %
3
0.1 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
5
99
0.5%
5
5.1%
90
90.9%
6
6.1 %
4
4.0 %
0
Parallel New Infection
5
247
1.1%
22
8.9%
220
89.1%
17
6.9 %
5
2.0 %
0
Parallel - Other
3
5
0.0%
0
0.0 %
4
80.0%
1
20.0 %
1
20.0 %
0
Parallel Persistent Infection
1
234
1.1%
11
4.7%
217
92.7%
16
6.8 %
6
2.6 %
0
Parallel - Rapid Testing Two-times IRs
5
8
0.0%
3
37.5%
5
62.5%
0
0
0
Wales - Intermediate TB
19
593
2.7%
41
6.9%
536
90.4%
40
6.7 %
16
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
1
2
0.0%
0
0.0 %
1
50.0%
1
50.0 %
1
50.0 %
0
Parallel New Infection
1
52
0.2%
2
3.8%
49
94.2%
4
7.7 %
1
1.9 %
0
Parallel - Other
2
18
0.1%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Wales - Low TB
4
72
0.3%
3
4.2%
65
90.3%
8
11.1 %
4
5.6 %
0
Scotland - Scotland
Parallel New Infection
1
16
0.1%
0
0.0 %
14
87.5%
5
31.3 %
2
12.5 %
0
Parallel Persistent Infection
1
338
1.5%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland - Scotland
2
354
1.6%
2
0.6%
343
96.9%
12
3.4 %
9
2.5 %
0
Submission Reason
No
submissions Samples
Gamma Positive
Gamma Negatives
Retest
Resample
Reject
n
% of total
n
%
n
%
n
%
n
%
n
%
GB
Extended Flexible IFN-GAMMA test
156
5402
2.4%
367
6.8%
4733
87.6%
276
5.1 %
201
3.7 %
101
1.9 %
Parallel - Herd or Group Slaughter
119
4435
2.0%
352
7.9%
3806
85.8%
345
7.8 %
270
6.1 %
8
0.2 %
Parallel New Infection
899 103483
46.1%
3628
3.5%
93027
89.9%
7677
7.4 %
6054
5.9 %
792
0.8 %
Parallel - new infection cull area
413
47094
21.0%
2383
5.1%
41822
88.8%
3240
6.9 %
2525
5.4 %
364
0.8 %
Parallel - Other
69
3959
1.8%
257
6.5%
3283
82.9%
457
11.5 %
377
9.5 %
42
1.1 %
Parallel Persistent Infection
389
45324
20.2%
1946
4.3%
40523
89.4%
2877
6.3 %
2216
4.9 %
642
1.4 %
Parallel - persistent infection cull area
68
13972
6.2%
778
5.6%
12399
88.7%
1007
7.2 %
788
5.6 %
7
0.1 %
Parallel - Rapid Testing Two-times IRs
305
886
0.4%
183
20.7%
633
71.4%
61
6.9 %
43
4.9 %
27
3.0 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
Total
2422 224581
100.0%
9894
4.4%
200251
89.2%
15941
7.1 %
12475
5.6 %
1983
0.9 %
England - HRA
Extended Flexible IFN-GAMMA test
22
1606
0.7%
79
4.9%
1422
88.5%
95
5.9 %
60
3.7 %
45
2.8 %
Parallel - Herd or Group Slaughter
4
33
0.0%
0
0.0 %
31
93.9%
3
9.1 %
2
6.1 %
0
Parallel New Infection
19
1643
0.7%
136
8.3%
1325
80.6%
228
13.9 %
182
11.1 %
0
Parallel - new infection cull area
412
46834
20.9%
2364
5.0%
41593
88.8%
3228
6.9 %
2513
5.4 %
364
0.8 %
Parallel - Other
20
1542
0.7%
83
5.4%
1172
76.0%
284
18.4 %
245
15.9 %
42
2.7 %
Parallel Persistent Infection
118
19743
8.8%
950
4.8%
17268
87.5%
1320
6.7 %
998
5.1 %
528
2.7 %
Parallel - persistent infection cull area
68
13972
6.2%
778
5.6%
12399
88.7%
1007
7.2 %
788
5.6 %
7
0.1 %
Serial - Anomalous Reaction
2
18
0.0%
0
0.0 %
17
94.4%
1
5.6 %
1
5.6 %
0
England - Edge
Parallel New Infection
674
84045
37.4%
2881
3.4%
75695
90.1%
6113
7.3 %
4802
5.7 %
685
0.8 %
Parallel - new infection cull area
1
260
0.1%
19
7.3%
229
88.1%
12
4.6 %
12
4.6 %
0
Parallel Persistent Infection
14
1314
0.6%
27
2.1%
1234
93.9%
66
5.0 %
53
4.0 %
0
Serial - Suspected NSR Herd
2
8
0.0%
0
0.0 %
8
100.0%
0
0
0
England - LRA
Extended Flexible IFN-GAMMA test
2
50
0.0%
1
2.0%
48
96.0%
3
6.0 %
1
2.0 %
0
Parallel - Herd or Group Slaughter
2
24
0.0%
8
33.3%
14
58.3%
2
8.3 %
2
8.3 %
0
Parallel New Infection
66
8165
3.6%
257
3.1%
7252
88.8%
758
9.3 %
618
7.6 %
38
0.5 %
Parallel Persistent Infection
5
736
0.3%
3
0.4%
561
76.2%
89
12.1 %
79
10.7 %
93
12.6 %

## Table 4B.  Summaryof Ifn Gamma Results By Submission Reason  (Total 2019) Cont.

England
1431 179993
80.1%
7586
4.2%
160268
89.0%
13209
7.3 %
10356
5.8 %
1802
1.0 %
Wales - High TB
Extended Flexible IFN-GAMMA test
93
2726
1.2%
194
7.1%
2434
89.3%
123
4.5 %
95
3.5 %
3
0.1 %
Parallel - Herd or Group Slaughter
107
4277
1.9%
327
7.6%
3689
86.3%
327
7.6 %
254
5.9 %
8
0.2 %
Parallel New Infection
29
1009
0.4%
56
5.6%
905
89.7%
55
5.5 %
47
4.7 %
1
0.1 %
Parallel - Other
33
1840
0.8%
122
6.6%
1619
88.0%
133
7.2 %
99
5.4 %
0
Parallel Persistent Infection
235
21183
9.4%
883
4.2%
19268
91.0%
1293
6.1 %
1013
4.8 %
21
0.1 %
Parallel - Rapid Testing Two-times IRs
265
773
0.3%
164
21.2%
542
70.1%
58
7.5 %
40
5.2 %
27
3.5 %
Wales - Intermediate TB
Extended Flexible IFN-GAMMA test
37
931
0.4%
89
9.6%
747
80.2%
51
5.5 %
42
4.5 %
53
5.7 %
Parallel - Herd or Group Slaughter
6
101
0.0%
17
16.8%
72
71.3%
13
12.9 %
12
11.9 %
0
Parallel New Infection
75
6018
2.7%
199
3.3%
5460
90.7%
395
6.6 %
307
5.1 %
52
0.9 %
Parallel - Other
14
559
0.2%
51
9.1%
477
85.3%
37
6.6 %
31
5.5 %
0
Parallel Persistent Infection
14
1581
0.7%
74
4.7%
1454
92.0%
85
5.4 %
53
3.4 %
0
Parallel - Rapid Testing Two-times IRs
38
111
0.0%
19
17.1%
89
80.2%
3
2.7 %
3
2.7 %
0
Wales - Low TB
Extended Flexible IFN-GAMMA test
2
89
0.0%
4
4.5%
82
92.1%
4
4.5 %
3
3.4 %
0
Parallel New Infection
22
731
0.3%
18
2.5%
674
92.2%
35
4.8 %
23
3.1 %
16
2.2 %
Parallel - Other
2
18
0.0%
1
5.6%
15
83.3%
3
16.7 %
2
11.1 %
0
Parallel Persistent Infection
2
429
0.2%
7
1.6%
409
95.3%
17
4.0 %
13
3.0 %
0
Parallel - Rapid Testing Two-times IRs
2
2
0.0%
0
0.0 %
2
100.0%
0
0
0
Wales
976
42378
18.9%
2225
5.3%
37938
89.5%
2632
6.2 %
2037
4.8 %
181
0.4 %
Scotland - Scotland
Parallel New Infection
14
1872
0.8%
81
4.3%
1716
91.7%
93
5.0 %
75
4.0 %
0
Parallel Persistent Infection
1
338
0.2%
2
0.6%
329
97.3%
7
2.1 %
7
2.1 %
0
Scotland
15
2210
1.0%
83
3.8%
2045
92.5%
100
4.5 %
82
3.7 %
0
| Year         | Month     |
|--------------|-----------|
| Herds        |           |
| sampled      |           |
| Samples      |           |
| tested       |           |
| IFNg+        |           |
| samples      |           |
| Wrong        |           |
| Eartags      |           |
| VL No        |           |
| Cult         |           |
| Herds        |           |
| with         |           |
| positives    |           |
| No PM        |           |
| No           |           |
| Cult         |           |
| No PM        |           |
| Cult         |           |
| Pend         |           |
| No PM        |           |
| Cult         |           |
| Neg          |           |
| No PM        |           |
| Cult         |           |
| Mb           |           |
| VL           |           |
| Cult         |           |
| Neg          |           |
| VL           |           |
| Cult         |           |
| Pend         |           |
| VL           |           |
| Cult         |           |
| Mb           |           |
| VL           |           |
| Cult         |           |
| Other        |           |
| NVL          |           |
| No           |           |
| Cult         |           |
| NVL          |           |
| Cult         |           |
| Pend         |           |
| NVL          |           |
| Cult         |           |
| Neg          |           |
| NVL          |           |
| Cult         |           |
| Mb           |           |
| NVL          |           |
| Cult         |           |
| Other        |           |
| No           |           |
| PM           |           |
| Cult         |           |
| Other        |           |
| 2018         | September |
| October      | 274       |
| November     | 282       |
| December     | 228       |
| 2019         | January   |
| February     | 320       |
| March        | 315       |
| April        | 269       |
| May          | 364       |
| June         | 287       |
| July         | 316       |
| August       | 262       |
| Totals (last |           |
| 12 months)   |           |
| 3422         | 316122    |
Country
Herds sampled
Samples tested
IFNg+ samples
VL No Cult
Herds with positives
 No PM No Cult
No PM Cult Pend
No PM Cult Neg
No PM Cult Mb
 VL Cult Neg
VL Cult Pend
VL Cult Mb
VL Cult Other
NVL No Cult
NVL Cult Pend
NVL Cult Neg
NVL Cult Mb
 NVL Cult Other
No PM Cult Other
England
1994
249158
1311
11207
179
0
0
0
0
928
13
11
35
3
9845
13
94
0
2
Scotland
17
2225
10
83
0
0
0
0
0
12
0
0
2
0
61
1
4
0
0
Wales
1411
64739
725
3788
71
0
0
0
0
162
8
3
16
1
2715
117
608
3
26
Totals
3422
316122
2046
15078
250
0
0
0
0
1102
21
14
53
4
12621
131
706
3
28

*Includes all animals with IFNg test negative, resample and reject outcomes

|                 | County   | %Resampled %Retested   |
|-----------------|----------|------------------------|
| Avon            | 6.67 %   | 6.67 %                 |
| Berks           | 10.00 %  | 10.77 %                |
| Bucks           | 20.43 %  | 22.58 %                |
| Carms           | 3.11 %   | 4.67 %                 |
| Ceredigion      | 3.98 %   | 6.42 %                 |
| Cheshire        | 5.07 %   | 7.93 %                 |
| Corn & Scilly   | 6.47 %   | 8.47 %                 |
| Cumbria         | 33.33 %  | 44.44 %                |
| Derbyshire      | 6.73 %   | 9.57 %                 |
| Devon           | 7.71 %   | 10.26 %                |
| Dorset          | 4.33 %   | 7.09 %                 |
| Dumfries & G    | 12.50 %  | 31.25 %                |
| Gloucestershire | 5.36 %   | 6.25 %                 |
| Gloucs          | 37.39 %  | 39.13 %                |
| Gr London       | 4.65 %   | 9.30 %                 |
| Gwent           | 22.22 %  | 22.22 %                |
| Hampshire       | 9.93 %   | 13.79 %                |
| Heref           | 3.00 %   | 3.67 %                 |
| Kirkcudbright   | 2.07 %   | 2.07 %                 |
| Leics & Rut     | 5.88 %   | 17.65 %                |
| NE Wales        | 3.05 %   | 7.32 %                 |
| Northants       |          |                        |
| Northumb        | 28.09 %  | 30.41 %                |
| N Yorks         | 3.98 %   | 5.61 %                 |
| Oxon            | 9.34 %   | 11.98 %   |
|-----------------|----------|-----------|
| Pembs           | 3.55 %   | 6.09 %    |
| Powys           | 3.45 %   | 6.03 %    |
| Shropshire      | 17.81 %  | 20.55 %   |
| Somerset excl N | 3.92 %   | 5.81 %    |
| Staffs          | 9.38 %   | 10.94 %   |
| Warks           | 4.92 %   | 6.03 %    |
| W Glamorgan     |          |           |
| Wiltshire       | 7.90 %   | 10.14 %   |
| W Sussex        | 60.87 %  | 65.22 %   |
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
17871
511
2.9%
18297
511
2.8%
Wales
3203
211
6.6%
3236
211
6.5%
Scotland
354
2
0.6%
354
2
0.6%
GB
21428
724
3.4%
21887
724
3.3%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
15428
592
3.8%
15697
592
3.8%
Wales
3771
87
2.3%
3849
87
2.3%
Scotland
49
3
6.1%
53
3
5.7%
GB
19248
682
3.5%
19599
682
3.5%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
165650
7586
4.6%
179993
7586
4.2%
Wales
38635
2225
5.8%
42378
2225
5.3%
Scotland
1889
83
4.4%
2210
83
3.8%
GB
206170
9894
4.8%
224581
9894
4.4%
Country
No. animals
No of IFHg+ animals
% Positive animals
No. samples
No of IFHg+ samples
England
110791
5999
5.4%
124473
5999
4.8%
Wales
31928
1306
4.1%
35246
1306
3.7%
Scotland
2066
181
8.8%
2226
181
8.1%
GB
144772
7486
5.2%
161945
7486
4.6%
% Positive samples
% Positive samples
% Positive samples
% Positive samples
* Test type
Num Submissions
Num Samples Num Positives
Private
% Positive
Devon
PRV-SP
1
1
0
0
Total
1
1
0
Isle of Man
SER-IOM
7
64
0
0
| % Positive          | * Test type   |
|---------------------|---------------|
| Num                 |               |
| Submissions         |               |
| Num                 |               |
| Samples             |               |
| Num                 |               |
| Positives           |               |
| Private             |               |
| Devon               | PRV-SE        |
| PRV-SP              | 1             |
| Gloucestershire     | PRV-SP        |
| Herefordshire       | PRV-SP        |
| Somerset excl North | PRV-SE        |
| PRV-SP              | 3             |
| Wiltshire           | PRV-SP        |
| Worcestershire      | SER-OTHER     |
| Total               |               |
| 13                  |               |
| 65                  |               |
| 15                  | 23.08 %       |
| Isle of Man         | SER-IOM       |

*SER-IOM: Serial High Specificity test - Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test - England

|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 4        |               146 | 12                   | 8.2%                              |
| Ceredigion       | 2               | 343      |                10 | 2.9%                 |                                   |
| North East Wales | 7               | 270      |                37 | 13.7%                |                                   |
| Pembrokeshire    | 2               | 70       |                 6 | 8.6%                 |                                   |
| Powys            | 2               | 45       |                 4 | 8.9%                 |                                   |
| South Wales      | 1               | 9        |                 0 | 0                    |                                   |
| Wiltshire        | 1               | 248      |                 9 | 3.6%                 |                                   |
| TC0077           | Sum:            | 1,131    |                78 | 6.9 %                |                                   |
|                  | Test            | County   |   Num Submissions | Num Samples Tested   | Num Positive Samples % Positive   |
|------------------|-----------------|----------|-------------------|----------------------|-----------------------------------|
| TC0077           | Carmarthenshire | 12       |               427 | 34                   | 8.0%                              |
| Ceredigion       | 3               | 372      |                17 | 4.6%                 |                                   |
| North East Wales | 19              | 737      |               103 | 14.0%                |                                   |
| Pembrokeshire    | 14              | 465      |                27 | 5.8%                 |                                   |
| Powys            | 6               | 313      |                18 | 5.8%                 |                                   |
| South Wales      | 5               | 196      |                16 | 8.2%                 |                                   |
| Wiltshire        | 1               | 248      |                 9 | 3.6%                 |                                   |
| TC0077           | Sum:            | 2,758    |               224 | 8.1 %                |                                   |