Monthly Report: Surveillance Project SB4008 IFNg tests for bovine tuberculosis

(TC0651 and TC0751)

Number 164

Report Period 1st - 30th April 2020

Issued 19 May 2020 Operational notes on terminology, definitions, re-test and re-samples test outcomes:

• “Samples” refers to an individual tube of blood taken from an individual animal which is subsequently submitted for testing. It is generally assumed that the number of samples and the number of animals are analogous as any duplicate sample blood tube from the same animal will not be tested. However, minor discrepancies may occur where the same animal is tested more than once due to a request for a resample or the animal is tested twice under different categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).

• “Submissions” refers to an individual batch or set of samples received for testing. Submissions may comprise 1 or more samples (for example, if an entire herd is being tested it is usually sent as 1 submission). It is generally assumed that the number of submissions and the number of herds are analogous.

• Retests are samples where the first ELISA assay fails and the same sample is retested on a new ELISA plate. Each sample can be retested only once. A retest is not a reportable test outcome.

• Resamples are where a sample has been retested and failed a second time, so that the lab requests a new sample. This is a reportable test outcome.

• Rejects are samples that are not tested by the lab for one of the following reasons: blood collected into wrong type of vacutainer, samples that have not been maintained at the appropriate temperature range (22±5°C), unlabelled samples, broken or cracked tubes, blood that is extensively clotted (small clots are OK), samples received after 4pm on the day after sample collection. Such samples are reported separately in the tables and in figure 5.

• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure of the quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) may indicate compromised blood quality as a result of collection/transportation conditions or due the animal having an unusually low/suppressed cellular immune response.

• NEG (no-antigen control) is a sample negative control which provides a measure of the background antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading) may indicate a laboratory procedures problem (normally resolved during re-test) or that an animal has unusually high background levels of IFN-g. | Submission Reason | Explanation | Mandatory? | |-------------------|-------------|------------| | Possible Herd Slaughter (PAR-HERD-S) | Parallel interferon-gamma blood testing of skin test negative cattle to inform whole or partial herd slaughters decisions | YES, if APHA are contemplating a herd slaughter | | Persistent TB breakdowns (OTF status withdrawn) (PAR-P-CONF) N.B. PAR-PERSIS from 1st April 2017 | Parallel blood testing of skin test-negative cattle in persistently infected herds that have failed to resolve by repeated short-interval skin testing and fulfill a minimum of biosecurity standards. | NO (APHA discretion) | | Parallel - Low Incidence (PAR-LOW-IN) N.B. PAR-NEW-IN from 1st April 2017 | Parallel blood testing to maximise the probability of removing all infected cattle in a new herd breakdown as soon as possible after confirmation of TB. | YES in Area of Low Incidence | | Rapid Testing of twice IR's (PAR-RAPID) | Parallel blood testing of two-times IRs identified under the severe interpretation of the skin test used in Wales. | YES (Wales only) | | PAR-CUL-N | Parallel blood testing to maximise the probability of removing all infected cattle in a new breakdown as soon as possible after confirmation of TB where herd is situated in badger control areas of England that have completed at least 2 effective culls. | YES (in eligible badger control areas of England) | | PAR-CUL-P | Parallel blood testing to maximise the probability of removing all infected cattle in a persistent confirmed breakdown where herd is situated within badger control areas of England that have completed at least 2 effective culls | NO (APHA discretion) | | Parallel Other (PAR-OTHER) | Other parallel blood testing not covered in any of the other scenarios | NO (APHA discretion) | | 'NSR' Herds (SER-NSR) | Modified serial blood testing of individual skin test reactors and/or IRs in unconfirmed TB breakdown herds to clarify their infection status where there is evidence of non-specific sensitisation to bovine tuberculin (the "non-specific reactor" procedure) | NO (APHA discretion) | | Suspected Fraud (SER-FRAUD) | Modified serial blood test of suspected fraudulent reactors to the skin test (animals with abnormal skin swellings), in confirmed or unconfirmed TB incidents. | NO (APHA discretion to firm up or rule out any suspicion of fraud and support any investigations) | | Serial Other (SER-OTHER) | Serial test – other reasons | NO (APHA Discretion) | | SER-FLEXI | Extended blood test to provide flexible test readout in a confirmed M. bovis-infected herd where Johne's (M. a. paratuberculosis) infection or vaccination is suspected to be interfering with M. bovis infection detection | NO (APHA discretion) | Table 1. Monthly Totals for April 2020

| Submissions | Num samples April 2020 | % | Total 2020 | % | |-------------|------------------------|---|------------|---| | England | 7482 | 68.47 % | 73317 | 78.36 % | | Wales | 2984 | 27.31 % | 17590 | 18.80 % | | Scotland | 462 | 4.23 % | 2662 | 2.84 % |

| Parallel Tests | PAR-CUL-N | 1461 | 13.37 % | 29044 | 31.04 % | |----------------|-----------|------|---------|-------|---------| | | PAR-CUL-P | 917 | 8.39 % | 4832 | 5.16 % | | | PAR-HERD-S| 28 | 0.26 % | 307 | 0.33 % | | | PAR-NEW-IN| 7338 | 67.15 % | 42815 | 45.76 % | | | PAR-OTHER | 133 | 1.22 % | 1755 | 1.88 % | | | PAR-PERSIS | 898 | 8.22 % | 12507 | 13.37 % | | | PAR-RAPID | 142 | 1.30 % | 441 | 0.47 % | | Total(% of all samples) | 10917 | 99.90 % | 91701 | 98.00 % |

| Serial/Extended Tests | SER-FLEXI | 11 | 0.10 % | 1868 | 2.00 % | |-----------------------|-----------|---|--------|------|--------| | | SER-FRAUD | 0 | 0.00 % | | | | | SER-NSR | 0 | 0.00 % | | | | Total(% of all samples) | 11 | 0.10 % | 1868 | 2.00 % |

| Retests | Total (% of all samples) | 687 | 6.29 % | 5930 | 6.34 % | |---------|--------------------------|------|--------|------|--------| | England | 482 | 4.41 % | 4495 | 4.80 % | | Wales | 187 | 1.71 % | 1288 | 1.38 % | | Scotland| 18 | 0.16 % | 147 | 0.16 % |

| Resamples | Total (% of all samples) | 535 | 4.90 % | 4678 | 5.00 % | |-----------|--------------------------|------|--------|------|--------| | England | 379 | 3.47 % | 3524 | 3.77 % | | Wales | 142 | 1.30 % | 1028 | 1.10 % | | Scotland | 14 | 0.13 % | 126 | 0.13 % |

| Rejects | Total (% of all samples) | 3 | 0.03 % | 1758 | 1.88 % | |---------|--------------------------|---|--------|------|--------| | England | 0 | 0.00 % | 1668 | 1.78 % | | Wales | 3 | 0.03 % | 25 | 0.03 % | | Scotland| 0 | 0.00 % | 65 | 0.07 % |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Figure 1. Number of samples submitted in each category during April 2020

Breakdown of Reason for submission

| Category | Number | Percentage | |-------------------|--------|------------| | PAR-CUL-N | 1461 | 13.4 % | | PAR-CUL-P | 917 | 8.4 % | | PAR-HERD-S | 28 | 0.3 % | | PAR-NEW-IN | 7338 | 67.1 % | | PAR-OTHER | 133 | 1.2 % | | PAR-PERSIS | 898 | 8.2 % | | PAR-RAPID | 142 | 1.3 % | | SER-FLEXI | 11 | 0.1 % | | **Total** | **10928** | **100.00 %** |

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne's infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Figure 2. Number of samples submitted in each category by County during April 2020

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-PERSIS: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Figure 3. Number of samples submitted in each category by County during 2020

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Table 2a. Summary by County for April 2020

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------------|--------------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % | n | % | n | % | | | England - HRA | Corn & Scilly| PAR-CUL-N | 5 | 240 | 10 4.17 % | 222 92.50 % | 11 4.58 % | 8 3.33 % | 0 0.00 % | | | | PAR-CUL-P | 2 | 638 | 7 1.10 % | 625 97.96 % | 13 2.04 % | 6 0.94 % | 0 0.00 % | | | | PAR-PERSIS | 1 | 84 | 1 1.19 % | 74 88.10 % | 10 11.90 % | 9 10.71 % | 0 0.00 % | | Devon | | PAR-CUL-N | 9 | 626 | 22 3.51 % | 573 91.53 % | 38 6.07 % | 31 4.95 % | 0 0.00 % | | Dorset | | PAR-CUL-N | 2 | 168 | 8 4.76 % | 151 89.88 % | 13 7.74 % | 9 5.36 % | 0 0.00 % | | | | PAR-CUL-P | 1 | 279 | 25 8.96 % | 235 84.23 % | 22 7.89 % | 19 6.81 % | 0 0.00 % | | | | PAR-PERSIS | 1 | 532 | 41 7.71 % | 449 84.40 % | 54 10.15 % | 42 7.89 % | 0 0.00 % | | Gloucs | | PAR-CUL-N | 3 | 404 | 22 5.45 % | 362 89.60 % | 22 5.45 % | 20 4.95 % | 0 0.00 % | | Shropshire | | PAR-CUL-N | 1 | 3 | 0 0.03 % | 3 100.00 % | 0 0.00 % | 0 0.00 % | 0 0.00 % | | Somerset excl N | | PAR-CUL-N | 4 | 20 | 2 10.00 % | 17 85.00 % | 1 5.00 % | 1 5.00 % | 0 0.00 % | | England - Edge| Berks | PAR-NEW-IN | 3 | 827 | 22 2.66 % | 749 90.57 % | 73 8.33 % | 56 6.77 % | 0 0.00 % | | | Bucks | PAR-NEW-IN | 1 | 24 | 0 0.00 % | 22 91.67 % | 3 12.50 % | 2 8.33 % | 0 0.00 % | | Cheshire | | PAR-NEW-IN | 9 | 1,157 | 25 2.16 % | 1074 92.83 % | 72 6.22 % | 58 5.01 % | 0 0.00 % | | Derbyshire | | PAR-NEW-IN | 7 | 941 | 31 3.29 % | 874 92.88 % | 39 4.14 % | 36 3.83 % | 0 0.00 % | | E Sussex | | PAR-NEW-IN | 2 | 435 | 12 2.76 % | 401 92.18 % | 24 5.52 % | 22 5.06 % | 0 0.00 % | | Leics & Rut | | PAR-NEW-IN | 2 | 218 | 4 1.83 % | 206 94.50 % | 19 8.72 % | 8 3.67 % | 0 0.00 % | | Northants | | PAR-NEW-IN | 2 | 36 | 1 2.78 % | 34 94.44 % | 2 5.56 % | 1 2.78 % | 0 0.00 % | | Oxon | | PAR-NEW-IN | 2 | 48 | 3 6.25 % | 41 85.42 % | 5 10.42 % | 4 8.33 % | 0 0.00 % | | Warks | | PAR-NEW-IN | 5 | 260 | 13 5.00 % | 229 88.08 % | 23 8.85 % | 18 6.92 % | 0 0.00 % | | England - LRA | Gr Manchester| PAR-NEW-IN | 1 | 2 | 0 0.02 % | 2 100.00 % | 0 0.00 % | 0 0.00 % | 0 0.00 % | | | N Yorks | PAR-NEW-IN | 4 | 540 | 5 0.93 % | 506 93.70 % | 36 7.04 % | 29 5.37 % | 0 0.00 % | | England | | | 67 | 7482 | 254 3.39 % | 6849 91.54 % | 482 6.44 % | 379 5.07 % | 0 0.00 % | | Wales - High TB| Carms | PAR-HERD-S | 2 | 27 | 2 7.41 % | 24 88.89 % | 1 3.70 % | 1 3.70 % | 0 0.00 % | | | | PAR-OTHER | 3 | 23 | 3 13.04 % | 18 78.26 % | 3 13.04 % | 2 8.70 % | 0 0.00 % | | | | PAR-PERSIS | 3 | 81 | 5 6.17 % | 72 88.89 % | 4 4.94 % | 4 4.94 % | 0 0.00 % | | | | PAR-RAPID | 9 | 33 | 5 15.15 % | 26 78.79 % | 3 9.09 % | 2 6.06 % | 0 0.00 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. | Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |--------------|--------------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % | n | % | n | % | | | Ceredigion | PAR-OTHER | 3 | 5 | 0.05 % | 0 | 0.00 % | 4 | 80.00 % | 1 | 20.00 % | 1 | 20.00 % | 0 | 0.00 % | | | PAR-PERSIS | 1 | 1 | 0.01 % | 0 | 0.00 % | 0 | 0.00 % | 1 | 100.00 %| 1 | 100.00 %| 0 | 0.00 % | | | PAR-RAPID | 6 | 25 | 0.23 % | 6 | 24.00 % | 16 | 64.00 % | 3 | 12.00 % | 3 | 12.00 % | 0 | 0.00 % | | | PAR-RAPID | 1 | 5 | 0.05 % | 1 | 20.00 % | 4 | 80.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Gwent | PAR-RAPID | 12 | 83 | 0.76 % | 17 | 20.48 % | 63 | 75.90 % | 3 | 3.61 % | 3 | 3.61 % | 0 | 0.00 % | | | PAR-PERSIS | 7 | 156 | 1.43 % | 8 | 5.13 % | 137 | 87.82 % | 12 | 7.69 % | 11 | 7.05 % | 0 | 0.00 % | | | PAR-RAPID | 16 | 52 | 0.48 % | 13 | 25.00 % | 33 | 63.46 % | 6 | 11.54 % | 6 | 11.54 % | 0 | 0.00 % | | Powys | PAR-HERD-S | 1 | 1 | 0.01 % | 0 | 0.00 % | 1 | 100.00 %| 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-PERSIS | 3 | 44 | 0.40 % | 3 | 6.82 % | 40 | 90.91 % | 1 | 2.27 % | 1 | 2.27 % | 0 | 0.00 % | | | PAR-RAPID | 6 | 9 | 0.08 % | 1 | 11.11 % | 7 | 77.78 % | 1 | 11.11 % | 1 | 11.11 % | 0 | 0.00 % | | | SER-FLEXI | 1 | 1 | 0.01 % | 0 | 0.00 % | 0 | 0.00 % | 1 | 100.00 %| 1 | 100.00 %| 0 | 0.00 % | | S Wales | PAR-RAPID | 1 | 2 | 0.02 % | 1 | 50.00 % | 1 | 50.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Wales - Intermediate TB | Carms | PAR-OTHER | 3 | 17 | 0.16 % | 3 | 17.65 % | 13 | 76.47 % | 1 | 5.88 % | 1 | 5.88 % | 0 | 0.00 % | | | NE Wales | PAR-NEW-IN | 12 | 902 | 8.25 % | 55 | 6.10 % | 795 | 88.14 % | 67 | 7.43 % | 52 | 5.76 % | 0 | 0.00 % | | | PAR-RAPID | 2 | 3 | 0.03 % | 0 | 0.00 % | 3 | 100.00 %| 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | SER-FLEXI | 2 | 10 | 0.09 % | 1 | 10.00 % | 9 | 90.00 % | 1 | 10.00 % | 0 | 0.00 % | 0 | 0.00 % | | Powys | PAR-NEW-IN | 1 | 183 | 1.67 % | 5 | 2.73 % | 170 | 92.90 % | 7 | 3.83 % | 7 | 3.83 % | 1 | 0.55 % | | S Glamorgan | PAR-NEW-IN | 1 | 1 | 0.01 % | 1 | 100.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Wales - Low TB | Gwynedd | PAR-NEW-IN | 8 | 765 | 7.00 % | 9 | 1.18 % | 730 | 95.42 % | 40 | 5.23 % | 24 | 3.14 % | 2 | 0.26 % | | | PAR-RAPID | 1 | 11 | 0.10 % | 0 | 0.00 % | 8 | 72.73 % | 3 | 27.27 % | 3 | 27.27 % | 0 | 0.00 % | | | PAR-NEW-IN | 5 | 538 | 4.92 % | 6 | 1.12 % | 514 | 95.54 % | 28 | 5.20 % | 18 | 3.35 % | 0 | 0.00 % | | | PAR-OTHER | 1 | 5 | 0.05 % | 0 | 0.00 % | 5 | 100.00 %| 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-RAPID | 1 | 1 | 0.01 % | 0 | 0.00 % | 1 | 100.00 %| 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Wales | | | 112 | 2984 | 27.31 % | 145 | 4.86 % | 2694 | 90.28 % | 187 | 6.27 % | 142 | 4.76 % | 3 | 0.10 % | | Scotland - Scotland | Argyll & Bute | PAR-NEW-IN | 1 | 16 | 0.15 % | 1 | 6.25 % | 9 | 56.25 % | 6 | 37.50 % | 6 | 37.50 % | 0 | 0.00 % | | | Kirkcudbright | PAR-NEW-IN | 2 | 85 | 0.78 % | 5 | 5.88 % | 78 | 91.76 % | 3 | 3.53 % | 2 | 2.35 % | 0 | 0.00 % | | | Wigtown | PAR-NEW-IN | 2 | 361 | 3.30 % | 3 | 0.83 % | 352 | 97.51 % | 9 | 2.49 % | 6 | 1.66 % | 0 | 0.00 % | | Scotland | | | 5 | 462 | 4.23 % | 9 | 1.95 % | 439 | 95.02 % | 18 | 3.90 % | 14 | 3.03 % | 0 | 0.00 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. Table 2a. Summary by County for April 2020 Cont.

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------|--------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | | Grand Total | | | Sum: | 184 | 10928 | 408 | 9982 | 687 | 535 | 3 |

\*For test criteria please refer to Operational notes.

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Table 2b. Summary by County for 2020

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------------|-----------------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | | England - HRA | Corn & Solly | PAR-CUL-N | 33 | 4,414 | 4.72 % | 143 | 3.24 % | 4087 | 92.59 % | 246 | 5.57 % | 184 | 4.17 % | 0 | 0.00 % | | | | PAR-CUL-P | 6 | 1,796 | 1.92 % | 45 | 2.51 % | 1696 | 94.43 % | 71 | 3.95 % | 55 | 3.06 % | 0 | 0.00 % | | | | PAR-PERSIS | 17 | 3,332 | 3.56 % | 135 | 4.05 % | 3079 | 92.41 % | 171 | 5.13 % | 117 | 3.51 % | 1 | 0.03 % | | Devon | | PAR-CUL-N | 98 | 10,914 | 11.66 % | 433 | 3.97 % | 9831 | 90.08 % | 675 | 6.18 % | 546 | 5.00 % | 104 | 0.95 % | | | | PAR-CUL-P | 7 | 726 | 0.78 % | 34 | 4.68 % | 633 | 87.19 % | 72 | 9.92 % | 59 | 8.13 % | 0 | 0.00 % | | | | PAR-PERSIS | 1 | 92 | 0.10 % | 8 | 8.70 % | 80 | 86.96 % | 8 | 8.70 % | 4 | 4.35 % | 0 | 0.00 % | | | | SER-FLEXI | 3 | 50 | 0.05 % | 2 | 4.00 % | 46 | 92.00 % | 2 | 4.00 % | 2 | 4.00 % | 0 | 0.00 % | | Dorset | | PAR-CUL-N | 20 | 2,440 | 2.61 % | 160 | 6.56 % | 2023 | 82.91 % | 132 | 5.41 % | 106 | 4.34 % | 151 | 6.19 % | | | | PAR-CUL-P | 6 | 1,440 | 1.54 % | 162 | 11.25 % | 1225 | 85.07 % | 80 | 5.56 % | 52 | 3.61 % | 1 | 0.07 % | | | | PAR-NEW-IN | 2 | 167 | 0.18 % | 5 | 2.99 % | 156 | 93.41 % | 11 | 6.59 % | 6 | 3.59 % | 0 | 0.00 % | | | | PAR-PERSIS | 1 | 532 | 0.57 % | 41 | 7.71 % | 449 | 84.40 % | 54 | 10.15 %| 42 | 7.89 % | 0 | 0.00 % | | | | SER-FLEXI | 5 | 740 | 0.79 % | 48 | 6.49 % | 674 | 91.08 % | 30 | 4.05 % | 18 | 2.43 % | 0 | 0.00 % | | Gloucs | | PAR-CUL-N | 25 | 2,409 | 2.57 % | 125 | 5.19 % | 2127 | 88.29 % | 189 | 7.85 % | 157 | 6.52 % | 0 | 0.00 % | | Heref | | PAR-CUL-N | 9 | 877 | 0.94 % | 33 | 3.76 % | 812 | 92.59 % | 43 | 4.90 % | 29 | 3.31 % | 3 | 0.34 % | | | | PAR-NEW-IN | 3 | 248 | 0.27 % | 10 | 4.03 % | 207 | 83.47 % | 38 | 15.32 %| 30 | 12.10 %| 1 | 0.40 % | | | | PAR-PERSIS | 2 | 213 | 0.23 % | 9 | 4.23 % | 172 | 80.75 % | 32 | 15.02 %| 32 | 15.02 %| 0 | 0.00 % | | Shropshire | | PAR-CUL-N | 3 | 416 | 0.44 % | 14 | 3.37 % | 385 | 92.55 % | 21 | 5.05 % | 15 | 3.81 % | 2 | 0.48 % | | | | PAR-PERSIS | 7 | 1,015 | 1.08 % | 60 | 5.91 % | 899 | 88.57 % | 65 | 6.40 % | 54 | 5.32 % | 2 | 0.20 % | | | | SER-FLEXI | 3 | 389 | 0.42 % | 16 | 4.11 % | 352 | 90.49 % | 27 | 6.94 % | 21 | 5.40 % | 0 | 0.00 % | | Somerset excl N | | PAR-CUL-N | 41 | 3,227 | 3.45 % | 119 | 3.69 % | 2972 | 92.10 % | 152 | 4.71 % | 108 | 3.35 % | 28 | 0.87 % | | | | PAR-CUL-P | 1 | 261 | 0.28 % | 13 | 4.98 % | 238 | 91.19 % | 13 | 4.98 % | 10 | 3.83 % | 0 | 0.00 % | | | | PAR-PERSIS | 2 | 286 | 0.31 % | 25 | 8.74 % | 253 | 88.46 % | 11 | 3.85 % | 8 | 2.80 % | 0 | 0.00 % | | Staffs | | PAR-CUL-N | 6 | 968 | 1.03 % | 37 | 3.82 % | 729 | 75.31 % | 51 | 5.27 % | 47 | 4.86 % | 155 | 16.01 %| | Wiltshire | | PAR-CUL-N | 26 | 2,965 | 3.17 % | 100 | 3.37 % | 2679 | 90.35 % | 226 | 7.62 % | 172 | 5.80 % | 14 | 0.47 % | | | | PAR-CUL-P | 4 | 609 | 0.65 % | 37 | 6.08 % | 547 | 89.82 % | 38 | 6.24 % | 25 | 4.11 % | 0 | 0.00 % | | | | PAR-PERSIS | 2 | 104 | 0.11 % | 5 | 4.81 % | 93 | 89.42 % | 7 | 6.73 % | 6 | 5.77 % | 0 | 0.00 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. Table 2b. Summary by County for 2020 Cont.

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------------|--------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | | England - Edge| Berks | PAR-NEW-IN | 14 | 1,716 | 1.83 % | 37 | 2.16 % | 1563 | 91.08 % | 151 | 8.80 % | 116 | 6.76 % | 0 | 0.00 % | 0 | 0.00 % | | | Bucks | PAR-NEW-IN | 9 | 970 | 1.04 % | 53 | 5.46 % | 836 | 86.19 % | 98 | 10.10 % | 81 | 8.35 % | 0 | 0.00 % | 0 | 0.00 % | | | Cheshire | PAR-NEW-IN | 68 | 11,036 | 11.79 % | 247 | 2.24 % | 10268 | 93.04 % | 582 | 5.27 % | 471 | 4.27 % | 50 | 0.45 % | 0 | 0.00 % | | | Derbyshire | PAR-NEW-IN | 54 | 6,745 | 7.21 % | 223 | 3.31 % | 5673 | 84.11 % | 486 | 7.21 % | 417 | 6.18 % | 432 | 6.40 % | 0 | 0.00 % | | | E Sussex | PAR-NEW-IN | 11 | 1,113 | 1.19 % | 42 | 3.77 % | 1031 | 92.63 % | 48 | 4.31 % | 40 | 3.59 % | 0 | 0.00 % | 0 | 0.00 % | | | Hampshire | PAR-CUL-N | 2 | 363 | 0.39 % | 15 | 4.13 % | 327 | 90.08 % | 39 | 10.74 % | 19 | 5.23 % | 2 | 0.55 % | 0 | 0.00 % | | | Leics & Rut | PAR-NEW-IN | 8 | 855 | 0.91 % | 21 | 2.46 % | 787 | 92.05 % | 52 | 6.08 % | 47 | 5.50 % | 0 | 0.00 % | 0 | 0.00 % | | | Northants | PAR-NEW-IN | 14 | 1,228 | 1.31 % | 39 | 3.18 % | 1124 | 91.53 % | 77 | 6.72 % | 49 | 3.99 % | 16 | 1.30 % | 0 | 0.00 % | | | Notts | PAR-NEW-IN | 1 | 75 | 0.08 % | 2 | 2.67 % | 72 | 96.00 % | 2 | 2.67 % | 1 | 1.33 % | 0 | 0.00 % | 0 | 0.00 % | | | Oxon | PAR-NEW-IN | 37 | 3,489 | 3.73 % | 142 | 4.07 % | 2896 | 83.00 % | 222 | 6.36 % | 163 | 4.67 % | 288 | 8.25 % | 0 | 0.00 % | | | Warks | PAR-NEW-IN | 28 | 2,882 | 3.08 % | 75 | 2.60 % | 2582 | 89.59 % | 157 | 5.45 % | 121 | 4.20 % | 104 | 3.61 % | 0 | 0.00 % | | England - LRA | Cumbria | PAR-NEW-IN | 1 | 30 | 0.03 % | 0 | 0.00 % | 29 | 96.67 % | 2 | 6.67 % | 1 | 3.33 % | 0 | 0.00 % | 0 | 0.00 % | | | Gr Manchester | PAR-NEW-IN | 5 | 268 | 0.29 % | 1 | 0.37 % | 54 | 20.15 % | 3 | 1.12 % | 3 | 1.12 % | 210 | 78.36 % | 0 | 0.00 % | | | Humberside | PAR-NEW-IN | 2 | 78 | 0.08 % | 7 | 8.97 % | 66 | 84.62 % | 7 | 8.97 % | 5 | 6.41 % | 0 | 0.00 % | 0 | 0.00 % | | | Lincs | PAR-NEW-IN | 3 | 176 | 0.19 % | 7 | 3.98 % | 162 | 92.05 % | 7 | 3.98 % | 7 | 3.98 % | 0 | 0.00 % | 0 | 0.00 % | | | Norfolk | PAR-NEW-IN | 4 | 49 | 0.05 % | 1 | 2.04 % | 29 | 59.18 % | 6 | 12.24 % | 6 | 12.24 % | 13 | 26.53 % | 0 | 0.00 % | | | N Yorks | PAR-NEW-IN | 7 | 755 | 0.81 % | 7 | 0.93 % | 628 | 83.18 % | 39 | 5.17 % | 29 | 3.84 % | 91 | 12.05 % | 0 | 0.00 % | | | Surrey | PAR-NEW-IN | 3 | 429 | 0.46 % | 17 | 3.96 % | 398 | 92.31 % | 18 | 4.20 % | 16 | 3.73 % | 0 | 0.00 % | 0 | 0.00 % | | England | | | 615 | 73317 | 78.36 % | 2769 | 3.78 % | 65356 | 89.14 % | 4495 | 6.13 % | 3524 | 4.81 % | 1668 | 22.8 % | 0 | 0.00 % | | Wales - High TB| Carms | PAR-HERD-S | 6 | 234 | 0.25 % | 19 | 8.12 % | 207 | 88.46 % | 15 | 6.41 % | 8 | 3.42 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-NEW-IN | | 1 | 8 | 0.01 % | 2 | 25.00 % | 6 | 75.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-OTHER | | 14 | 234 | 0.25 % | 31 | 13.25 % | 186 | 79.49 % | 19 | 8.12 % | 17 | 7.28 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-PERSIS | | 24 | 1,547 | 1.65 % | 109 | 7.05 % | 1385 | 89.53 % | 73 | 4.72 % | 51 | 3.30 % | 2 | 0.13 % | 0 | 0.00 % | | | PAR-RAPID | | 28 | 119 | 0.13 % | 21 | 17.65 % | 89 | 74.79 % | 13 | 10.92 % | 9 | 7.56 % | 0 | 0.00 % | 0 | 0.00 % | | | SER-FLEXI | | 2 | 27 | 0.03 % | 7 | 25.93 % | 20 | 74.07 % | 1 | 3.70 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. Table 2b. Summary by County for 2020 Cont.

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------------|--------------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % | n | % | n | % | n | | Ceredigion | PAR-HERD-S | 1 | 4 | 0.00 % | 0 | 0.00 % | 4 | 100.00 % | 0 | 0.00 % | | | PAR-OTHER | 8 | 26 | 0.03 % | 0 | 0.00 % | 23 | 88.46 % | 3 | 11.54 %| | | PAR-PERSIS | 16 | 429 | 0.46 % | 19 | 4.43 % | 390 | 90.91 % | 22 | 5.13 % | | | PAR-RAPID | 13 | 56 | 0.06 % | 17 | 30.36 % | 35 | 62.50 % | 4 | 7.14 % | | Gwent | PAR-HERD-S | 1 | 3 | 0.00 % | 1 | 33.33 % | 2 | 66.67 % | 0 | 0.00 % | | | PAR-PERSIS | 13 | 541 | 0.58 % | 29 | 5.36 % | 495 | 91.50 % | 32 | 5.91 % | | | PAR-RAPID | 10 | 22 | 0.02 % | 6 | 27.27 % | 16 | 72.73 % | 0 | 0.00 % | | | SER-FLEXI | 3 | 133 | 0.14 % | 17 | 12.78 % | 113 | 84.96 % | 7 | 5.26 % | | Powys | PAR-HERD-S | 8 | 31 | 0.03 % | 3 | 9.68 % | 28 | 90.32 % | 2 | 6.45 % | | | PAR-PERSIS | 9 | 747 | 0.80 % | 34 | 4.55 % | 639 | 85.54 % | 82 | 10.98 %| | | PAR-RAPID | 13 | 36 | 0.04 % | 5 | 13.16 % | 32 | 84.21 % | 1 | 2.63 % | | | SER-FLEXI | 10 | 212 | 0.23 % | 17 | 8.02 % | 186 | 87.74 % | 19 | 8.96 % | | S Wales | PAR-PERSIS | 1 | 69 | 0.07 % | 3 | 4.35 % | 64 | 92.75 % | 6 | 8.70 % | | | PAR-RAPID | 1 | 2 | 0.00 % | 1 | 50.00 % | 1 | 50.00 % | 0 | 0.00 % | | | SER-FLEXI | 1 | 36 | 0.04 % | 0 | 0.00 % | 36 | 100.00 % | 0 | 0.00 % | | W Glamorgan | PAR-PERSIS | 1 | 3 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 3 | 100.00 %| | | PAR-RAPID | 2 | 14 | 0.01 % | 0 | 0.00 % | 12 | 85.71 % | 3 | 21.43 %| | | SER-FLEXI | 1 | 9 | 0.01 % | 1 | 11.11 % | 8 | 88.89 % | 0 | 0.00 % | | Wales - Inter | PAR-HERD-S | 3 | 30 | 0.03 % | 5 | 16.67 % | 24 | 80.00 % | 3 | 10.00 %| | | PAR-PERSIS | 4 | 18 | 0.02 % | 3 | 16.67 % | 14 | 77.78 % | 1 | 5.56 % | | | PAR-RAPID | 3 | 196 | 0.21 % | 23 | 11.62 % | 167 | 84.34 % | 8 | 4.04 % | | | PAR-PERSIS | 3 | 3 | 0.00 % | 2 | 66.67 % | 1 | 33.33 % | 0 | 0.00 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017); persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017); parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Table 2b. Summary by County for 2020 Cont.

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------------|--------------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | | Ceredigion | PAR-PERSIS | 2 | 7 | 0.01 % | 4 | 57.14 % | 2 | 28.57 % | 1 | 14.29 % | 1 | 14.29 % | 0 | 0.00 % | | | PAR-RAPID | 1 | 1 | 0.00 % | 1 | 100.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | SER-FLEXI | 2 | 48 | 0.05 % | 6 | 12.50 % | 40 | 83.33 % | 2 | 4.17 % | 2 | 4.17 % | 0 | 0.00 % | | NE Wales | PAR-NEW-IN | 36 | 4,065 | 4.37 % | 130 | 3.18 % | 3687 | 90.26 % | 306 | 7.49 % | 268 | 6.56 % | 0 | 0.00 % | | | PAR-OTHER | 1 | 15 | 0.02 % | 2 | 13.33 % | 13 | 86.67 % | 1 | 6.67 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-PERSIS | 10 | 477 | 0.51 % | 61 | 12.79 % | 401 | 84.07 % | 20 | 4.19 % | 15 | 3.14 % | 0 | 0.00 % | | | PAR-RAPID | 7 | 26 | 0.03 % | 3 | 11.54 % | 21 | 80.77 % | 2 | 7.69 % | 2 | 7.69 % | 0 | 0.00 % | | | SER-FLEXI | 7 | 129 | 0.14 % | 6 | 4.65 % | 114 | 88.37 % | 12 | 9.30 % | 7 | 5.43 % | 2 | 1.55 % | | Powys | PAR-NEW-IN | 7 | 764 | 0.82 % | 13 | 1.70 % | 715 | 93.59 % | 45 | 5.89 % | 35 | 4.58 % | 1 | 0.13 % | | | PAR-OTHER | 1 | 12 | 0.01 % | 0 | 0.00 % | 10 | 83.33 % | 3 | 25.00 % | 2 | 16.67 % | 0 | 0.00 % | | S Glamorgan | PAR-RAPID | 1 | 1 | 0.00 % | 1 | 100.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Wales - Low TB| Gwynedd | PAR-NEW-IN | 15 | 1.075 % | 13 | 1.21 % | 1018 | 94.70 % | 63 | 5.86 % | 41 | 3.81 % | 3 | 0.28 % | | | PAR-OTHER | 1 | 1 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | 1 | 100.00 %| 1 | 100.00 %| 0 | 0.00 % | | | PAR-RAPID | 2 | 12 | 0.01 % | 0 | 0.00 % | 9 | 75.00 % | 3 | 25.00 % | 3 | 25.00 % | 0 | 0.00 % | | NE Wales | PAR-NEW-IN | 10 | 1,578 | 1.69 % | 19 | 1.20 % | 1490 | 94.42 % | 102 | 6.46 % | 69 | 4.37 % | 0 | 0.00 % | | | PAR-OTHER | 1 | 5 | 0.01 % | 0 | 0.00 % | 5 | 100.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | | PAR-PERSIS | 1 | 40 | 0.04 % | 0 | 0.00 % | 32 | 80.00 % | 10 | 25.00 % | 8 | 20.00 % | 0 | 0.00 % | | | PAR-RAPID | 1 | 1 | 0.00 % | 0 | 0.00 % | 1 | 100.00 % | 0 | 0.00 % | 0 | 0.00 % | 0 | 0.00 % | | Wales | | | 471 | 17590 | 18.80 % | 984 | 5.59 % | 15553 | 88.42 % | 1288 | 7.32 % | 1028 | 5.84 % | 25 | 0.14 % | | Scotland - Scotland | Argyll & Bute | PAR-NEW-IN | 7 | 588 | 0.63 % | 32 | 5.44 % | 455 | 77.38 % | 41 | 6.97 % | 36 | 6.12 % | 65 | 11.05 % | | | Ayrshire | PAR-NEW-IN | 3 | 383 | 0.41 % | 1 | 0.26 % | 359 | 93.73 % | 30 | 7.83 % | 23 | 6.01 % | 0 | 0.00 % | | | Berwickshire | PAR-NEW-IN | 2 | 78 | 0.08 % | 8 | 10.26 % | 62 | 79.49 % | 8 | 10.26 % | 8 | 10.26 % | 0 | 0.00 % | | | Dumfries & G | PAR-NEW-IN | 6 | 849 | 0.91 % | 12 | 1.41 % | 802 | 94.46 % | 38 | 4.48 % | 35 | 4.12 % | 0 | 0.00 % | | | Kirkcudbright| PAR-NEW-IN | 3 | 106 | 0.11 % | 5 | 4.72 % | 99 | 93.40 % | 3 | 2.83 % | 2 | 1.89 % | 0 | 0.00 % | | | Wigtown | PAR-NEW-IN | 5 | 658 | 0.70 % | 17 | 2.58 % | 619 | 94.07 % | 27 | 4.10 % | 22 | 3.34 % | 0 | 0.00 % | | Scotland | | | 26 | 2662 | 2.84 % | 75 | 2.82 % | 2396 | 90.01 % | 147 | 5.52 % | 126 | 4.73 % | 65 | 2.44 % |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. Table 2b. Summary by County for 2020 Cont.

| Country | County | Submission Reasons\* | No. subs | Samples | Gamma Positive | Gamma Negatives | Retest\* | Resample | Reject | |---------|--------|---------------------|----------|---------|----------------|----------------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | | Grand Total | | Sum: | 1112 | 93569 | 3828 | 83305 | 5930 | 4678 | 1758 |

\*For test criteria please refer to Operational notes.

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.

PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other Table 3a. Summary of IFN Gamma results by country and protocol April 2020

| Country | Protocol | No submissions | Samples | Positive | Negatives | Retest\* | Resample | Reject | |---------|----------------|----------------|---------|----------|-----------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | n | % | | England | Parallel | 67 | 7482 | 100.0% | 254 | 3.4% | 6849 | 91.5% | 482 | 6.4% | 379 | 5.1% | 0 | 0.0% | | | Serial/Extended| 0 | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | | | Total | 67 | 7482 | 100.0% | 254 | 3.4% | 6849 | 91.5% | 482 | 6.4% | 379 | 5.1% | 0 | 0.0% | | Scotland| Parallel | 5 | 462 | 100.0% | 9 | 1.9% | 439 | 95.0% | 18 | 3.9% | 14 | 3.0% | 0 | 0.0% | | | Serial/Extended| 0 | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | | | Total | 5 | 462 | 100.0% | 9 | 1.9% | 439 | 95.0% | 18 | 3.9% | 14 | 3.0% | 0 | 0.0% | | Wales | Parallel | 109 | 2973 | 99.6% | 144 | 4.8% | 2685 | 90.3% | 185 | 6.2% | 141 | 4.7% | 3 | 0.1% | | | Serial/Extended| 3 | 11 | 0.4% | 1 | 9.1% | 9 | 81.8% | 2 | 18.2% | 1 | 9.1% | 0 | 0.0% | | | Total | 112 | 2984 | 100.0% | 145 | 4.9% | 2694 | 90.3% | 187 | 6.3% | 142 | 4.8% | 3 | 0.1% | | GB | Parallel | 181 | 10917 | 99.9% | 407 | 3.7% | 9973 | 91.4% | 685 | 6.3% | 534 | 4.9% | 3 | 0.0% | | | Serial/Extended| 3 | 11 | 0.1% | 1 | 9.1% | 9 | 81.8% | 2 | 18.2% | 1 | 9.1% | 0 | 0.0% | | | Total | 184 | 10928 | 100.0% | 408 | 3.7% | 9982 | 91.3% | 687 | 6.3% | 535 | 4.9% | 3 | 0.0% |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. Table 3b. Summary of IFN Gamma results by country and protocol (Total 2020)

| Country | Protocol | No submissions | Samples | Positive | Negatives | Retest\* | Resample | Reject | |---------|----------------|----------------|---------|----------|-----------|---------|----------|--------| | | | | n | % of total | n | % | n | % | n | % | n | % | | England | Parallel | 602 | 72093 | 98.3% | 2699 | 3.7% | 64243 | 89.1% | 4436 | 6.2% | 3483 | 4.8% | 1668 | 2.3% | | | Serial/Extended| 13 | 1224 | 1.7% | 70 | 5.7% | 1113 | 90.9% | 59 | 4.8% | 41 | 3.3% | 0 | 0.0% | | | Total | 615 | 73317 | 100.0% | 2769 | 3.8% | 65356 | 89.1% | 4495 | 6.1% | 3524 | 4.8% | 1668 | 2.3% | | Scotland| Parallel | 26 | 2662 | 100.0% | 75 | 2.8% | 2396 | 90.0% | 147 | 5.5% | 126 | 4.7% | 65 | 2.4% | | | Serial/Extended| 0 | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | 0 | 0.0% | | | Total | 26 | 2662 | 100.0% | 75 | 2.8% | 2396 | 90.0% | 147 | 5.5% | 126 | 4.7% | 65 | 2.4% | | Wales | Parallel | 439 | 16946 | 96.3% | 925 | 5.5% | 14993 | 88.5% | 1243 | 7.3% | 1005 | 5.9% | 23 | 0.1% | | | Serial/Extended| 32 | 644 | 3.7% | 59 | 9.2% | 560 | 87.0% | 45 | 7.0% | 23 | 3.6% | 2 | 0.3% | | | Total | 471 | 17590 | 100.0% | 984 | 5.6% | 15553 | 88.4% | 1288 | 7.3% | 1028 | 5.8% | 25 | 0.1% | | GB | Parallel | 1067 | 91701 | 98.0% | 3699 | 4.0% | 81632 | 89.0% | 5826 | 6.4% | 4614 | 5.0% | 1756 | 1.9% | | | Serial/Extended| 45 | 1868 | 2.0% | 129 | 6.9% | 1673 | 89.6% | 104 | 5.6% | 64 | 3.4% | 2 | 0.1% | | | Total | 1112 | 93569 | 100.0% | 3828 | 4.1% | 83305 | 89.0% | 5930 | 6.3% | 4678 | 5.0% | 1758 | 1.9% |

- Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratory to 're-test' the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only. | Submission Reason | No submissions | Samples | Gamma Positive | Gamma Negatives | Retest | Resample | Reject | |-------------------|----------------|---------|----------------|----------------|--------|----------|--------| | | n | % of total | n | % | n | % | n | % | n | % | n | % | | GB | | | | | | | | | | | | | | Extended Flexible IFN-GAMMA test | 3 | 11 | 0.1% | 1 | 9.1% | 9 | 81.8% | 2 | 18.2% | 1 | 9.1% | 0 | | | Parallel - Herd or Group Slaughter | 3 | 28 | 0.3% | 2 | 7.1% | 25 | 89.3% | 1 | 3.6% | 1 | 3.6% | 0 | | | Parallel New Infection | 69 | 7338 | 0.7% | 200 | 2.7% | 6765 | 92.5% | 458 | 6.2% | 349 | 4.8% | 3 | 0.0% | | Parallel - new infection cull area | 24 | 1461 | 13.4% | 64 | 4.4% | 1328 | 90.9% | 85 | 5.8% | 69 | 4.7% | 0 | | | Parallel - Other | 22 | 133 | 1.2% | 23 | 17.3% | 103 | 77.4% | 8 | 6.0% | 7 | 5.3% | 0 | | | Parallel Persistent Infection | 16 | 898 | 8.2% | 58 | 6.5% | 772 | 86.0% | 82 | 9.1% | 68 | 7.6% | 0 | | | Parallel - persistent infection cull area | 3 | 917 | 8.4% | 32 | 3.5% | 860 | 93.8% | 35 | 3.8% | 25 | 2.7% | 0 | | | Parallel - Rapid Testing Two-times IRs | 44 | 142 | 1.3% | 28 | 19.7% | 99 | 69.7% | 16 | 11.3% | 15 | 10.6% | 0 | | | Total | 184 | 10928 | 100.0% | 408 | 3.7% | 9982 | 91.3% | 687 | 6.3% | 535 | 4.9% | 3 | 0.0% | | England - HRA | | | | | | | | | | | | | | Parallel - new infection cull area | 24 | 1461 | 13.4% | 64 | 4.4% | 1328 | 90.9% | 85 | 5.8% | 69 | 4.7% | 0 | | | Parallel Persistent Infection | 2 | 616 | 5.6% | 42 | 6.8% | 523 | 84.9% | 64 | 10.4% | 51 | 8.3% | 0 | | | Parallel - persistent infection cull area | 3 | 917 | 8.4% | 32 | 3.5% | 860 | 93.8% | 35 | 3.8% | 25 | 2.7% | 0 | | | England - HRA | 29 | 2994 | 27.4% | 138 | 4.6% | 2711 | 90.5% | 184 | 6.1% | 145 | 4.8% | 0 | | | England - Edge | | | | | | | | | | | | | | Parallel New Infection | 33 | 3946 | 36.1% | 111 | 2.8% | 3630 | 92.0% | 260 | 6.6% | 205 | 5.2% | 0 | | | England - LRA | | | | | | | | | | | | | | Parallel New Infection | 5 | 542 | 5.0% | 5 | 0.9% | 508 | 93.7% | 38 | 7.0% | 29 | 5.4% | 0 | | | Wales - High TB | | | | | | | | | | | | | | Extended Flexible IFN-GAMMA test | 1 | 1 | 0.0% | 0 | 0.0% | 0 | 0.0% | 1 | 100.0% | 1 | 100.0% | 0 | | | Parallel - Herd or Group Slaughter | 3 | 28 | 0.3% | 2 | 7.1% | 25 | 89.3% | 1 | 3.6% | 1 | 3.6% | 0 | | | Parallel - Other | 18 | 111 | 1.0% | 20 | 18.0% | 85 | 76.6% | 7 | 6.3% | 6 | 5.4% | 0 | | | Parallel Persistent Infection | 14 | 282 | 2.6% | 16 | 5.7% | 249 | 88.3% | 18 | 6.4% | 17 | 6.0% | 0 | | | Parallel - Rapid Testing Two-times IRs | 39 | 126 | 1.2% | 27 | 21.4% | 87 | 69.0% | 13 | 10.3% | 12 | 9.5% | 0 | | | Wales - High TB | 75 | 548 | 5.0% | 65 | 11.9% | 446 | 81.4% | 40 | 7.3% | 37 | 6.8% | 0 | | | Wales - Intermediate TB | | | | | | | | | | | | | | Extended Flexible IFN-GAMMA test | 2 | 10 | 0.1% | 1 | 10.0% | 9 | 90.0% | 1 | 10.0% | 0 | 0 | | | Parallel New Infection | 13 | 1085 | 9.9% | 60 | 5.5% | 965 | 88.9% | 74 | 6.8% | 59 | 5.4% | 1 | 0.1% | | Parallel - Other | 3 | 17 | 0.2% | 3 | 17.6% | 13 | 76.6% | 1 | 5.9% | 1 | 5.9% | 0 | | | Parallel - Rapid Testing Two-times IRs | 3 | 4 | 0.0% | 1 | 25.0% | 3 | 75.0% | 0 | 0 | 0 | 0 | 0 | | | Wales - Intermediate TB | 21 | 1116 | 10.2% | 65 | 5.8% | 990 | 88.7% | 76 | 6.8% | 60 | 5.4% | 1 | 0.1% | | Wales - Low TB | | | | | | | | | | | | | | Parallel New Infection | 13 | 1303 | 11.9% | 15 | 1.2% | 1244 | 95.5% | 68 | 5.2% | 42 | 3.2% | 2 | 0.2% | | Parallel - Other | 1 | 5 | 0.0% | 0 | 0.0% | 5 | 100.0% | 0 | 0 | 0 | 0 | 0 | | | Parallel - Rapid Testing Two-times IRs | 2 | 12 | 0.1% | 0 | 0.0% | 9 | 75.0% | 3 | 25.0% | 3 | 25.0% | 0 | | | Wales - Low TB | 16 | 1320 | 12.1% | 15 | 1.1% | 1258 | 95.3% | 71 | 5.4% | 45 | 3.4% | 2 | 0.2% | | Scotland - Scotland | | | | | | | | | | | | | | Parallel New Infection | 5 | 462 | 4.2% | 9 | 1.9% | 439 | 95.0% | 18 | 3.9% | 14 | 3.0% | 0 | | | Scotland - Scotland | 5 | 462 | 4.2% | 9 | 1.9% | 439 | 95.0% | 18 | 3.9% | 14 | 3.0% | 0 | | | Submission Reason | No submissions | Samples | Gamma Positive | Gamma Negatives | Retest | Resample | Reject | |--------------------------------------------------------|----------------|---------|----------------|-----------------|--------|----------|--------| | | n | % of total | n | % | n | % | n | % | n | % | | GB | | | | | | | | | Extended Flexible IFN-GAMMA test | 45 | 1868 | 2.0% | 129 | 6.9% | 1673 | 89.6% | 104 | 5.6% | 64 | 3.4% | 2 | 0.1% | | Parallel - Herd or Group Slaughter | 21 | 307 | 0.3% | 29 | 9.4% | 268 | 87.3% | 21 | 6.8% | 10 | 3.3% | 0 | | | Parallel New Infection | 376 | 42815 | 45.8% | 1197 | 2.8% | 38177 | 89.2% | 2693 | 6.3% | 2167 | 5.1% | 1274 | 3.0% | | Parallel - new infection cull area | 265 | 29044 | 31.0% | 1180 | 4.1% | 26014 | 89.6% | 1784 | 6.1% | 1391 | 4.8% | 459 | 1.6% | | Parallel - Other | 76 | 1755 | 1.9% | 152 | 8.7% | 1471 | 83.8% | 153 | 8.7% | 132 | 7.5% | 0 | | | Parallel Persistent Infection | 186 | 12507 | 13.4% | 757 | 6.1% | 11042 | 88.3% | 871 | 7.0% | 686 | 5.5% | 22 | 0.2% | | Parallel - persistent infection cull area | 24 | 4832 | 5.2% | 291 | 6.0% | 4339 | 89.8% | 274 | 5.7% | 201 | 4.2% | 1 | 0.0% | | Parallel - Rapid Testing Two-times IRs | 119 | 441 | 0.5% | 93 | 21.1% | 321 | 72.8% | 30 | 6.8% | 27 | 6.1% | 0 | | | Total | 1112 | 93569 | 100.0% | 3828 | 4.1% | 83305 | 89.0% | 5930 | 6.3% | 4678 | 5.0% | 1758 | 1.9% | | England - HRA | | | | | | | | | Extended Flexible IFN-GAMMA test | 13 | 1224 | 1.3% | 70 | 5.7% | 1113 | 90.9% | 59 | 4.8% | 41 | 3.3% | 0 | | | Parallel New Infection | 5 | 415 | 0.4% | 15 | 3.6% | 363 | 87.5% | 49 | 11.8%| 36 | 8.7% | 1 | 0.2% | | Parallel - new infection cull area | 261 | 28630 | 30.6% | 1164 | 4.1% | 25645 | 89.6% | 1735 | 6.1% | 1364 | 4.8% | 457 | 1.6% | | Parallel Persistent Infection | 32 | 5574 | 6.0% | 283 | 5.1% | 5025 | 90.2% | 348 | 6.2% | 263 | 4.7% | 3 | 0.1% | | Parallel - persistent infection cull area | 24 | 4832 | 5.2% | 291 | 6.0% | 4339 | 89.8% | 274 | 5.7% | 201 | 4.2% | 1 | 0.0% | | England - Edge | | | | | | | | | Parallel New Infection | 251 | 30443 | 32.5% | 890 | 2.9% | 27138 | 89.1% | 1899 | 6.2% | 1525 | 5.0% | 890 | 2.9% | | Parallel - new infection cull area | 4 | 414 | 0.4% | 16 | 3.9% | 369 | 89.1% | 49 | 11.8%| 27 | 6.5% | 2 | 0.5% | | England - LRA | | | | | | | | | Parallel New Infection | 25 | 1765 | 1.9% | 40 | 2.2% | 1364 | 76.4% | 82 | 4.6% | 67 | 3.8% | 314 | 17.6% | | England | 615 | 73317 | 78.4% | 2769 | 3.8% | 65356 | 89.1% | 4495 | 6.1% | 3524 | 4.8% | 1668 | 2.3% | | Wales - High TB | | | | | | | | | Extended Flexible IFN-GAMMA test | 20 | 454 | 0.5% | 42 | 9.3% | 398 | 87.7% | 31 | 6.8% | 14 | 3.1% | 0 | | | Parallel - Herd or Group Slaughter | 18 | 277 | 0.3% | 24 | 8.7% | 244 | 88.1% | 18 | 6.5% | 9 | 3.2% | 0 | | | Parallel New Infection | 1 | 8 | 0.0% | 2 | 25.0% | 6 | 75.0% | 0 | 0 | 0 | 0 | 0 | | | Parallel - Other | 68 | 1704 | 1.8% | 147 | 8.6% | 1429 | 83.9% | 147 | 8.6% | 128 | 7.5% | 0 | | | Parallel Persistent Infection | 138 | 6211 | 6.6% | 386 | 6.2% | 5415 | 87.2% | 484 | 7.8% | 391 | 6.3% | 19 | 0.3% | | Parallel - Rapid Testing Two-times IRs | 104 | 397 | 0.4% | 86 | 21.7% | 289 | 72.8% | 25 | 6.3% | 22 | 5.5% | 0 | | | Wales - Intermediate TB | | | | | | | | | Extended Flexible IFN-GAMMA test | 12 | 190 | 0.2% | 17 | 8.9% | 162 | 85.3% | 14 | 7.4% | 9 | 4.7% | 2 | 1.1% | | Parallel - Herd or Group Slaughter | 3 | 30 | 0.0% | 5 | 16.7% | 24 | 80.0% | 3 | 10.0%| 1 | 3.3% | 0 | | | Submission Reason | Total 2020 | Wales - Low TB | Scotland - Scotland | Scotland | Wales | |-----------------------------------|------------|----------------|---------------------|----------|-------| | | | | | | | | Parallel New Infection | 43 | 25 | 26 | 26 | 471 | | | 4849 | 2653 | 2662 | 2662 | 17590 | | | 5.2% | 2.8% | 2.8% | 2.8% | 18.8% | | | 143 | 32 | 75 | 75 | 984 | | | 2.9% | 1.2% | 2.8% | 2.8% | 5.6% | | | 4402 | 2508 | 2396 | 2396 | 15553 | | | 90.8% | 94.5% | 90.0% | 90.0% | 88.4% | | | 351 | 165 | 147 | 147 | 1288 | | | 7.2% | 6.2% | 5.5% | 5.5% | 7.3% | | | 303 | 110 | 126 | 126 | 1028 | | | 6.2% | 4.1% | 4.7% | 4.7% | 5.8% | | | 1 | 3 | 65 | 65 | 25 | | | 0.0% | 0.1% | 2.4% | 2.4% | | | Parallel - Other | 6 | 2 | 26 | 26 | | | | 45 | 6 | 2662 | 2662 | | | | 0.0% | 0.0% | 2.8% | 2.8% | | | | 5 | 0 | 75 | 75 | | | | 11.1% | 0.0% | 2.8% | 2.8% | | | | 37 | 5 | 2396 | 2396 | | | | 82.2% | 83.6% | 90.0% | 90.0% | | | | 5 | 29 | 147 | 147 | | | | 11.1% | 4.3% | 5.5% | 5.5% | | | | 3 | 24 | 126 | 126 | | | | 6.7% | 3.5% | 4.7% | 4.7% | | | | 0 | 0 | 65 | 65 | | | Parallel Persistent Infection | 15 | 1 | 26 | 26 | | | | 682 | 40 | 2662 | 2662 | | | | 0.7% | 0.0% | 2.8% | 2.8% | | | | 88 | 0 | 75 | 75 | | | | 12.9% | 0.0% | 2.8% | 2.8% | | | | 570 | 10 | 2396 | 2396 | | | | 83.6% | 76.9% | 90.0% | 90.0% | | | | 29 | 3 | 147 | 147 | | | | 4.3% | 23.1% | 5.5% | 5.5% | | | | 24 | 3 | 126 | 126 | | | | 3.5% | 23.1% | 4.7% | 4.7% | | | | 0 | 0 | 65 | 65 | | | Parallel - Rapid Testing Two-times IRs | 12 | 3 | 26 | 26 | | | | 31 | 13 | 2662 | 2662 | | | | 0.0% | 0.0% | 2.8% | 2.8% | | | | 7 | 0 | 75 | 75 | | | | 22.6% | 0.0% | 2.8% | 2.8% | | | | 22 | 10 | 2396 | 2396 | | | | 71.0% | 76.9% | 90.0% | 90.0% | | | | 2 | 3 | 147 | 147 | | | | 6.5% | 23.1% | 5.5% | 5.5% | | | | 2 | 3 | 126 | 126 | | | | 6.5% | 23.1% | 4.7% | 4.7% | | | | 0 | 0 | 65 | 65 | | | Wales - Low TB | | | | | | | Parallel New Infection | 25 | 25 | 26 | 26 | 471 | | | 2653 | 2653 | 2662 | 2662 | 17590 | | | 2.8% | 2.8% | 2.8% | 2.8% | 18.8% | | | 32 | 32 | 75 | 75 | 984 | | | 1.2% | 1.2% | 2.8% | 2.8% | 5.6% | | | 2508 | 2508 | 2396 | 2396 | 15553 | | | 94.5% | 94.5% | 90.0% | 90.0% | 88.4% | | | 165 | 165 | 147 | 147 | 1288 | | | 6.2% | 6.2% | 5.5% | 5.5% | 7.3% | | | 110 | 110 | 126 | 126 | 1028 | | | 4.1% | 4.1% | 4.7% | 4.7% | 5.8% | | | 3 | 3 | 65 | 65 | 25 | | | 0.1% | 0.1% | 2.4% | 2.4% | | | Wales | | | | | | | Scotland - Scotland | | | | | | | Parallel New Infection | 26 | 26 | 26 | 26 | | | | 2662 | 2662 | 2662 | 2662 | | | | 2.8% | 2.8% | 2.8% | 2.8% | | | | 75 | 75 | 75 | 75 | | | | 2.8% | 2.8% | 2.8% | 2.8% | | | | 2396 | 2396 | 2396 | 2396 | | | | 90.0% | 90.0% | 90.0% | 90.0% | | | | 147 | 147 | 147 | 147 | | | | 5.5% | 5.5% | 5.5% | 5.5% | | | | 126 | 126 | 126 | 126 | | | | 4.7% | 4.7% | 4.7% | 4.7% | | | | 65 | 65 | 65 | 65 | | | | 2.4% | 2.4% | 2.4% | 2.4% | | Table 5a. Summary of PM and culture results for IFNg positive animals.

| Year | Month | Herds sampled | Samples tested | Herds with positives | IFNg+ samples | Wrong Eartags | No PM Cult | No PM Cult Pend | No PM Cult Neg | No PM Cult Mb | No PM Cult Other | VL No Cult | VL Cult | VL Cult Pend | VL Cult Mb | VL Cult Other | NVL No Cult | NVL Cult | NVL Cult Pend | NVL Cult Neg | NVL Cult Mb | NVL Cult Other | |------|-------|---------------|----------------|----------------------|---------------|---------------|------------|----------------|----------------|---------------|----------------|------------|--------|-------------|-----------|-------------|-------------|---------|---------------|-------------|-----------|---------------|-------------|---------|---------------| | 2019 | May | 364 | 28357 | 176 | 1116 | 2 | 0 | 0 | 0 | 0 | 0 | 99 | 0 | 0 | 5 | 0 | 962 | 6 | 42 | 0 | 0 | 0 | | | June | 287 | 26287 | 159 | 964 | 5 | 13 | 0 | 0 | 0 | 0 | 94 | 1 | 0 | 4 | 0 | 773 | 0 | 73 | 0 | 0 | 0 | | | July | 316 | 32549 | 201 | 1219 | 1 | 13 | 0 | 0 | 0 | 0 | 108 | 3 | 0 | 12 | 0 | 1020 | 0 | 58 | 0 | 2 | 0 | | | August| 262 | 21887 | 126 | 724 | 1 | 8 | 0 | 0 | 0 | 0 | 84 | 1 | 0 | 6 | 0 | 570 | 0 | 52 | 0 | 0 | 0 | | | September | 245 | 25317 | 134 | 693 | 1 | 7 | 0 | 0 | 0 | 0 | 94 | 1 | 0 | 3 | 0 | 542 | 0 | 45 | 0 | 0 | 0 | | | October| 298 | 26690 | 154 | 743 | 0 | 0 | 0 | 0 | 0 | 0 | 58 | 1 | 0 | 5 | 0 | 604 | 0 | 71 | 0 | 3 | 0 | | | November| 272 | 30186 | 169 | 1080 | 14 | 0 | 0 | 0 | 0 | 0 | 72 | 5 | 0 | 15 | 0 | 910 | 0 | 61 | 0 | 1 | 0 | | | December| 234 | 25192 | 138 | 904 | 2 | 0 | 0 | 0 | 0 | 0 | 87 | 1 | 0 | 4 | 1 | 759 | 0 | 49 | 0 | 1 | 0 | | 2020 | January| 343 | 31274 | 227 | 1367 | 1 | 7 | 0 | 0 | 0 | 0 | 178 | 3 | 0 | 8 | 0 | 1110 | 1 | 57 | 0 | 0 | 0 | | | February| 323 | 33146 | 192 | 1289 | 1 | 21 | 0 | 0 | 0 | 0 | 187 | 0 | 0 | 3 | 6 | 982 | 2 | 87 | 0 | 0 | 0 | | | March | 262 | 18221 | 147 | 764 | 1 | 28 | 0 | 0 | 0 | 0 | 100 | 0 | 6 | 2 | 0 | 591 | 35 | 1 | 0 | 0 | 0 | | | April | 184 | 10928 | 96 | 408 | 2 | 138 | 0 | 0 | 0 | 0 | 28 | 0 | 1 | 1 | 0 | 216 | 22 | 0 | 0 | 0 | 0 | | | Totals (last 12 months) | 3390 | 310034 | 1919 | 11271 | 15 | 251 | 0 | 0 | 0 | 0 | 1189 | 16 | 7 | 68 | 7 | 9039 | 66 | 596 | 0 | 7 | 0 | Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)

| Country | Herds sampled | Samples tested | Herds with positives | IFNg+ samples | No PM No Cult | No PM Cult Pend | No PM Cult Neg | No PM Cult Mb | No PM Cult Other | VL No Cult | VL Cult Neg | VL Cult Pend | VL Cult Mb | VL Cult Other | NVL No Cult | NVL Cult Pend | NVL Cult Neg | NVL Cult Mb | NVL Cult Other | |---------|---------------|----------------|----------------------|---------------|---------------|----------------|----------------|---------------|----------------|------------|-------------|-------------|------------|---------------|------------|---------------|-------------|------------|----------------| | England | 1951 | 245696 | 1194 | 8276 | 158 | 0 | 0 | 0 | 0 | 1049 | 8 | 2 | 43 | 2 | 6935 | 4 | 54 | 0 | 1 | | Scotland| 45 | 4989 | 25 | 161 | 4 | 0 | 0 | 0 | 0 | 24 | 0 | 0 | 4 | 0 | 111 | 15 | 2 | 0 | 0 | | Wales | 1394 | 59349 | 700 | 2834 | 89 | 0 | 0 | 0 | 0 | 116 | 8 | 5 | 21 | 5 | 1993 | 47 | 540 | 0 | 6 | | Totals | 3390 | 310034 | 1919 | 11271 | 251 | 0 | 0 | 0 | 0 | 1189 | 16 | 7 | 68 | 7 | 9039 | 66 | 596 | 0 | 7 | Figure 4a. Number of herds sampled for IFNg and numbers of herds with any positive IFNg results.

Figure 4b. Number of animals sampled for IFNg and numbers of animals with positive IFNg results.

\*Includes all animals with IFNg test negative, resample and reject outcomes Figure 5. Rates of resamples including the fail rates broken down for positive (POS) and negative (NEG) controls. Figure 6. Proportion of samples retested, or requiring a resample, during April 2020 (% submission per County)

| County | %Resampled | %Retested | |-------------------------|------------|-----------| | Argyll & Bute | 37.50 % | 37.50 % | | Berks | 6.77 % | 8.83 % | | Bucks | 8.33 % | 12.50 % | | Carms | 5.52 % | 6.63 % | | Ceredigion | 16.13 % | 16.13 % | | Cheshire | 5.01 % | 6.22 % | | Corn & Scilly | 2.39 % | 3.53 % | | Derbyshire | 3.83 % | 4.14 % | | Devon | 4.95 % | 6.07 % | | Dorset | 7.15 % | 9.09 % | | E Sussex | 5.06 % | 5.52 % | | Gloucs | 4.95 % | 5.45 % | | Gr Manchester | | | | Gwent | 3.48 % | 5.54 % | | Gwynedd | | | | Kirkcudbright | 2.35 % | 3.53 % | | Leics & Rut | 3.67 % | 8.72 % | | NE Wales | 4.80 % | 6.58 % | | Northants | 2.78 % | 5.56 % | | N Yorks | 5.37 % | 7.04 % | | Oxon | 8.33 % | 10.42 % | | Pembs | 6.87 % | 7.22 % | | Powys | 4.20 % | 4.20 % | | S Glamorgan | | | | Shropshire | | | | Somerset excl N | 5.00 % | 5.00 % | | S Wales | | | | Warks | 6.92 % | 8.85 % | | Wigtown | 1.66 % | 2.49 % |

### Table 6a (i): Number of animals and submissions tested in April 2020

| Country | No. animals | No of IFHg+ animals | % Positive animals | No. samples | No of IFHg+ samples | % Positive samples | |---------|-------------|---------------------|--------------------|-------------|---------------------|--------------------| | England | 7439 | 254 | 3.4% | 7482 | 254 | 3.4% | | Wales | 2923 | 145 | 5.0% | 2984 | 145 | 4.9% | | Scotland| 461 | 9 | 2.0% | 462 | 9 | 1.9% | | GB | 10823 | 408 | 3.8% | 10928 | 408 | 3.7% |

### Table 6a (ii): Number of animals and submissions tested in April 2019

| Country | No. animals | No of IFHg+ animals | % Positive animals | No. samples | No of IFHg+ samples | % Positive animals | |---------|-------------|---------------------|--------------------|-------------|---------------------|--------------------| | England | 23451 | 918 | 3.9% | 23776 | 918 | 3.9% | | Wales | 6535 | 217 | 3.3% | 6544 | 217 | 3.3% | | GB | 29986 | 1135 | 3.8% | 30320 | 1135 | 3.7% |

### Table 6b (i): Number of animals and submissions tested in 2020 Up to end of April

| Country | No. animals | No of IFHg+ animals | % Positive animals | No. samples | No of IFHg+ samples | % Positive samples | |---------|-------------|---------------------|--------------------|-------------|---------------------|--------------------| | England | 69064 | 2769 | 4.0% | 73317 | 2769 | 3.8% | | Wales | 16751 | 983 | 5.9% | 17590 | 984 | 5.6% | | Scotland| 2517 | 75 | 3.0% | 2662 | 75 | 2.8% | | GB | 88332 | 3827 | 4.3% | 93569 | 3828 | 4.1% |

### Table 6b (ii): Number of animals and submissions tested in 2019 Up to end of April

| Country | No. animals | No of IFHg+ animals | % Positive animals | No. samples | No of IFHg+ samples | % Positive animals | |---------|-------------|---------------------|--------------------|-------------|---------------------|--------------------| | England | 86743 | 4519 | 5.2% | 91395 | 4519 | 4.9% | | Wales | 22260 | 1348 | 6.1% | 23740 | 1348 | 5.7% | | Scotland| 366 | 4 | 1.1% | 366 | 4 | 1.1% | | GB | 109369 | 5871 | 5.4% | 115501 | 5871 | 5.1% |

### Table 7a Number of private samples April 2020

| Test type | Num Submissions | Num Samples | Num Positives Private | % Positive | |-----------|-----------------|-------------|-----------------------|------------| | Total | 0 | 0 | 0 | 0 |

### Table 7b Number of private samples 2020

| Location | Test type | Num Submissions | Num Samples | Num Positives Private | % Positive | |----------------|-----------|-----------------|-------------|-----------------------|------------| | Cheshire | PRV-SP | 1 | 1 | 0 | 0 | | Devon | PRV-SE | 1 | 119 | 0 | 0 | | | PRV-SP | 1 | 6 | 0 | 0 | | Dorset | PRV-SE | 1 | 1 | 0 | 0 | | | PRV-SP | 1 | 3 | 0 | 0 | | Gloucestershire| PRV-SE | 1 | 8 | 3 | 37.50 % | | Oxfordshire | PRV-SP | 1 | 32 | 0 | 0 | | Staffordshire | PRV-SE | 1 | 24 | 0 | 0 | | Worcestershire | PRV-SP | 1 | 1 | 0 | 0 | | Total | | 9 | 195 | 3 | 1.54 % | | Isle of Man | SER-IOM | 8 | 46 | 0 | 0 |

*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England; PRV-SP: Private High Specificity test – England*

### Table 8a Number of Cattle Antibody Tests April 2020

| Test | County | Num Submissions | Num Samples Tested | Num Positive Samples | % Positive | |-------|-------------------|-----------------|--------------------|----------------------|------------| | TC0077| Carmarthenshire | 4 | 29 | 5 | 17.2% | | | Ceredigion | 3 | 19 | 3 | 15.8% | | | North East Wales | 2 | 10 | 1 | 10.0% | | | Pembrokeshire | 12 | 86 | 22 | 25.6% | | | Powys | 2 | 20 | 2 | 10.0% | | | South Wales | 2 | 7 | 4 | 57.1% | | TC0077| | 25 | 171 | 37 | 21.6% |

### Table 8b Number of Cattle Antibody Tests 2020

| Test | County | Num Submissions | Num Samples Tested | Num Positive Samples | % Positive | |-------|-------------------|-----------------|--------------------|----------------------|------------| | TC0077| Carmarthenshire | 12 | 245 | 37 | 15.1% | | | Ceredigion | 7 | 26 | 4 | 15.4% | | | Devon | 4 | 1,032 | 95 | 9.2% | | | North East Wales | 14 | 515 | 55 | 10.7% | | | Pembrokeshire | 28 | 443 | 78 | 17.6% | | | Powys | 4 | 103 | 13 | 12.6% | | | South Wales | 10 | 149 | 34 | 22.8% | | TC0077| | 79 | 2,513 | 316 | 12.6% |