LOCUS       PIGCN21_AN   7263 BP SS-DNA   CIRCULAR  SYN       24-SEP-2002
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
COMMENT     Primers used to PCR amplify the IRES-eGFPcre-FRT-neo-FRT cassette:

                  Primer-L   GCCAAGCTATCGAATTCCGCC

                  Primer-R   CTATTCCAGAAGTAGTGAGGA
FEATURES             Location/Qualifiers
      misc_feature    2259..2844
                      /note="ECMV IRES "
      CDS             129..989
                      /note="Amp"
      CDS             2845..4667
                      /note="eGFPcre fusion protein"
      misc_feature    complement(4680..4713)
                      /note="FRT"
      CDS             5157..5951
                      /note="Neo"
      misc_feature    complement(6031..6064)
                      /note="FRT"
BASE COUNT     1782 A   1872 C   1968 G   1641 T      0 OTHER
ORIGIN      -
         1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
        61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
       121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
       181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
       241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
       301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
       361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
       421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
       481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
       541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
       601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
       661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
       721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
       781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
       841 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
       901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
       961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
      1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
      1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
      1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
      1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
      1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
      1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
      1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
      1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
      1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
      1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
      1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
      1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
      1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
      1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG
      1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG
      1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT
      1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG
      2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT
      2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
      2161 CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT GGTACCGGGC CCCCCCTCGA
      2221 GGTCGACCTG CAGCCAAGCT ATCGAATTCC GCCCCCCCCC CTCTCCCTCC CCCCCCCCTA
      2281 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT
      2341 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA
      2401 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG
      2461 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT
      2521 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT
      2581 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG
      2641 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG
      2701 TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTACAC ATGCTTTACA TGTGTTTAGT
      2761 CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA
      2821 CACGATGATA ATATGGCCAC AACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
      2881 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
      2941 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
      3001 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
      3061 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
      3121 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
      3181 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
      3241 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
      3301 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
      3361 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
      3421 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC
      3481 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
      3541 GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCTGCA
      3601 TCCAATTTAC TGACCGTACA CCAAAATTTG CCTGCATTAC CGGTCGATGC AACGAGTGAT
      3661 GAGGTTCGCA AGAACCTGAT GGACATGTTC AGGGATCGCC AGGCGTTTTC TGAGCATACC
      3721 TGGAAAATGC TTCTGTCCGT TTGCCGGTCG TGGGCGGCAT GGTGCAAGTT GAATAACCGG
      3781 AAATGGTTTC CCGCAGAACC TGAAGATGTT CGCGATTATC TTCTATATCT TCAGGCGCGC
      3841 GGTCTGGCAG TAAAAACTAT CCAGCAACAT TTGGGCCAGC TAAACATGCT TCATCGTCGG
      3901 TCCGGGCTGC CACGACCAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GCGGATCCGA
      3961 AAAGAAAACG TTGATGCCGG TGAACGTGCA AAACAGGCTC TAGCGTTCGA ACGCACTGAT
      4021 TTCGACCAGG TTCGTTCACT CATGGAAAAT AGCGATCGCT GCCAGGATAT ACGTAATCTG
      4081 GCATTTCTGG GGATTGCTTA TAACACCCTG TTACGTATAG CCGAAATTGC CAGGATCAGG
      4141 GTTAAAGATA TCTCACGTAC TGACGGTGGG AGAATGTTAA TCCATATTGG CAGAACGAAA
      4201 ACGCTGGTTA GCACCGCAGG TGTAGAGAAG GCACTTAGCC TGGGGGTAAC TAAACTGGTC
      4261 GAGCGATGGA TTTCCGTCTC TGGTGTAGCT GATGATCCGA ATAACTACCT GTTTTGCCGG
      4321 GTCAGAAAAA ATGGTGTTGC CGCGCCATCT GCCACCAGCC AGCTATCAAC TCGCGCCCTG
      4381 GAAGGGATTT TTGAAGCAAC TCATCGATTG ATTTACGGCG CTAAGGATGA CTCTGGTCAG
      4441 AGATACCTGG CCTGGTCTGG ACACAGTGCC CGTGTCGGAG CCGCGCGAGA TATGGCCCGC
      4501 GCTGGAGTTT CAATACCGGA GATCATGCAA GCTGGTGGCT GGACCAATGT AAATATTGTC
      4561 ATGAACTATA TCCGTAACCT GGATAGTGAA ACAGGGGCAA TGGTGCGCCT GCTGGAAGAT
      4621 GGCGATTCTC GAGGTACCGC GGGCCCGGGA TCCACCGGAT CTAGATAACT GATCAGCTTG
      4681 AAGTTCCTAT ACTTTCTAGA GAATAGGAAC TTCGGAATAG GAACTTCAAG ATCCCCCTGG
      4741 CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCGTCAGGTG
      4801 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
      4861 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
      4921 AGAGTCCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC
      4981 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG
      5041 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
      5101 GTCAGTAACC ATAGTCCGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
      5161 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
      5221 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
      5281 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
      5341 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
      5401 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
      5461 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
      5521 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
      5581 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
      5641 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
      5701 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
      5761 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG
      5821 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG
      5881 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG
      5941 AGTTCTTCTG AGGGGATCGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT
      6001 TTGTTCGGAT CCGAGCTTCA AAAGCGCTCT GAAGTTCCTA TACTTTCTAG AGAATAGGAA
      6061 CTTCGGAATA GTAACTTCTC CATGGTAGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA
      6121 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG
      6181 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG
      6241 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC
      6301 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC
      6361 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGCCTCGC
      6421 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC
      6481 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG
      6541 CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATGGCCGCCA
      6601 CCGCGGTGGA GCTCCAATTC GCCCTATAGT GAGTCGTATT ACAATTCACT GGCCGTCGTT
      6661 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT
      6721 CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG
      6781 TTGCGCAGCC TGAATGGCGA ATGGAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT
      6841 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT
      6901 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
      6961 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG
      7021 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
      7081 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
      7141 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
      7201 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
      7261 CAG
//