January 2, 1941

Dr. Hawe& %tmw
The Rouk,f*lle,r Poondattoa
49 nest 49th street
Eew fork, l'ht Tort

?Smt let me gay that the work fa orgrenfcr ah8ZRistXy +snd rtx'uutural

uh8mietry Is going al5QJ wll.  &Kthm8i#t6r~ with tw pO6bdOutQX%te a6#sistk%IitS

aad faur .$raduata rtadenta, tr a&i= hrradwby en 68Yercal ppfobl8nir.  ES h&B

fittsd into om' dmprlYttp8Sd 6dktiSf43CtOril~, an& thaw hasl barn tu) tronbl@ est

all of the aoti that remstiraasr acu6span~ss ths appointment of a ~psan

prof86aor.  earl 8bmnz1 im rorfria& ar hard as 8ver and SS rffw%ively.  'he

mo~bau.br 6tXUUt~8 pro&ram fr botming &~ncr;. %e orystlal rtructurc. of

CbAinQ, nOW COstplOtQd, h2k6 tWX!8d ant; to be %6p%Ci%11t illtQr%6tin#J.  In m.6

pd.ypaptl&~, with fifty or one hundred adno add resltdusr per aofecmb, an4

f thbk that w 6hall -566d ia &na; 556@&6 6t=5tWo dstaX%if~tf5Rs Of

the68.  At pre8uat w are rtwlying p5ly&.3-&n5s, and %. E%aall, I&O is

horo this pas aa &aggsnheim Prellrzw, %rr now prepar%n(p 00s~ pslypeptiib

contafning both &yefne an8 er&nine.  f am confident that thir wttbod of

attaak, usindg 6ysth8tga ~02jptQtid86 of kBOwn aa;riZko sCic% 5CW@OsbtiOB, whi5h
might bs callsd iwtifiafal pmteln6, till lead ultPmat4.y to the eelution of

ths ptotefa problem.

     For abeut six m5nth6 Dr. D&n C+ur@elhr, Rcrukefeller %llow, sod

Dr. Ovid Pr%ssnran have been uorki.ng with an6 09 experimntal pnobltms in


Dr. Warren Weaver

J~taaly 2, 1941

Campbell was here.  Dr. Prwtwan, an c~~gaxtic uhrstirrt trained harat, was

6qqWt8d fn part frem the ht3kefellsr 8'oadatioa rand ia part from tha %y%a

hnh Of the bt%tUt(l. Ah together we hawr used about $m of this yede

Rodmfellsr bnbgst for thr work in lmammlog3r. sphe mmmrahar oompleted OP

unaer way am the follow.%n&

1,  Serological mdiim6 with 6hp16 mb&Au8a 0OntdAi~ two or WOTQ

argmimt for the framwork theory of sntibo8gcantigen preaipitetss.

2.  The manuftdmw of antfbedire $33 vitro from noma smm proteins.

fn my p%piw E 6Ugp6t6d that St &OUM be pO86tble to mmkfa?tura antibodias

in vitro by denaturing aenam prot+lnt aad oliawly renaturing them in the

nlsing thx'em diff8r8nt frsationr of beef &@obxLkin and the Q-6 nmthy~ blm LB

anti&pm.  tt WaB found thert iaftsr d6natUmtion of t&t pP0tQiA with i?&a~i


Dr. l&wren WeAv-ar

-9           Jwry 2, 1941

or by h%at 81OV mnAtmtdOn ia the prQ68&08 of the asr, p~&UOba a pmtein

solution which oosabined with the &ye nmh~~olp8 rk.@y that protein wlutionr

obtalmd in other #eye, ti 8r6 bJ? l'libpid r8Zilt%ttrrratiOn in pZ"%`Wl0Q Of a;rs Or

by wrrieu~ traataentm in dW8ll58 of dye,  SImilas expm%wmts uerb startad

here with bacteria aa antigem, an8 Dr. Cerrngbsll psSas to centhue them in

Ohicago.  Further work %s aa~ded to tact the quwiflaiiq of the artifiulaUg

pro&cad aritigmr, but the ??66tit6 obtained a;lreWrdJ ~LZW po%ttim

  3. h quantitatim ttu* of mra hmologotkr to antigens ctontalaiall; tvo

different haptasalc groupr.  Landrtaln8r rtvparteb 6~erinssrtts qgestfbg thsbt

no antlbody w4wml.r of type A'-B' wwe prersent fn the serum ppodumd by

swPtigenr uontalnfng two dilffareat gzmpr A em& a. Thlrr rwult ia inco~~~~tible

with my thaam or en;p theory ilrv52dag bivalmt or nm.lt;Evabmt aAtlboAgP

B51CWlb8.  With th8 5Gapemttrsn of Dr. bncZUt5in8F, M ha?`@ been oarryine;

out quiibntftat%W &ttodilrS 59 bin ~6z%, a&d ham, obtafzmd -tit8 which strp~.mrt

ths oonclusion upporfts to that ruaohed by him.

   4.  The thiaom thist trp;elatlnwtSo#a fs the result af the olarspfnip to@athar

of otrlls by bimlant antibo&y molemlsr requipeo that fah%bition of agE;lutizmtion

ocmur 5zll tm&tR8nt of o83.1# by a insiefrsntly StZ'@A@ a&glutSxria fmlution.

Etooker an& Bayd ban reported fisflnr$ to pro&106 ~k&iwtfan fn sgthsxytes

an8 wgtz8 aeuorbingiy aga.inaQ tha b~pal~t azktibody theory. We are mwry%ng

out aggltatlnation experWmtr uehg anttarsRnilic ecctd ream aa orglntfnln sbnd

rrythFo~88 trsata0 wLth d%aaotirad armilic aaid as ths oslle.

     Them are lttallp other exparimente which WB p&m to aamy out. Thnm

inclndr, the qwmtltertive rtudy of antibamttm m&position of prwipft&tss,

the de'ten"iDbition of eqtzilibmdus WBi6tWIt6 and heats of X'&%&foA~ by

physiml-chsaalc~l rethodr, srpecialky by the um of vatiour baptma for ths


Dr. %%rren weaver           -4m            JanuAry 2, 1941


-5            Jwmey 2, 1941

Eqansee of risktlR& prafessrrr

      1000.00

2otaZ $20 ,OOO.#

O~~~pball, at p-awe AasiBtant Profsaeor of .%mtexiology and Inmmolog$ at

the `hlveraity of Chicago.  Ha is a good teahniolan in thb field, an industrious

ana with an open mind ragardiag wisnce.  X doubt that a better m.n oatid be

pmpsration of' orgenfo substances thle year lq two ueniore in ahemistrgr, the

The Item of $75QQ.O0 for agparaturr, %xpplieq, anin%al% woula penn~t U% to uw

cad &mld psmitz calw the sonetxuctfos of a Ttseliue apparatue for tho