Identification_Information:
  Citation:
    Citation_Information:
      Originator: U.S. Geological Survey, National Wetlands Research Center (NWRC)
      Publication_Date: Unpublished material
      Title: Nutritional Analysis of Fruits and Flowers Data
      Geospatial_Data_Presentation_Form: digital tabular data
  Description:
    Abstract:
      With funding provided by the US Fish and Wildlife Service, the US
      Geological Survey, National Wetlands Research Center is conducting an
      ongoing study to develop a database containing nutritional information
      for fruits, flowers, and insects in regards to bird populations;
      currently the study includes samples from the southeastern and
      southcentral regions of the United States (Texas,Louisiana, Alabama,
      and Mississippi) with plans to expand the collection to include
      nationwide samples. The database contains physical analysis of
      specimens to include size and weight measurements, and water content
      for the whole specimen and for the pulp. Chemical analyses include
      measuring caloric value, percent of inorganic matter, and mineral
      content and analyzing sugars, proteins, and fatty acids levels.
      Samples for analyses come from different sources; NWRC personnel
      collect specimens while concurrently working on other projects, or
      specimens are sent to NWRC by other entities for analyses.
    Purpose:
      To determine nutritional content of fruits, flowers, and insects.
      Secondarily, to develop a cost structure for bird populations.
    Supplemental_Information:
      Please contact the principal investigator for specific details on the
      lab analyses methodology. The information provided in the methodology
      section is general with some specifics. It is advisable that further
      information be obtained before using any of the methodologies.
  Time_Period_of_Content:
    Time_Period_Information:
      Range_of_Dates/Times:
        Beginning_Date: 2002
        Ending_Date: Present
    Currentness_Reference: Ground condition
  Status:
    Progress: In work
    Maintenance_and_Update_Frequency: irregularly
  Spatial_Domain:
    Description_of_Geographic_Extent: Southeastern and Southcentral US
    Bounding_Coordinates:
      West_Bounding_Coordinate: -106.631
      East_Bounding_Coordinate: -84.783
      North_Bounding_Coordinate: 36.500
      South_Bounding_Coordinate: 25.833
  Keywords:
    Theme:
      Theme_Keyword_Thesaurus: None
      Theme_Keyword: chemical analysis
      Theme_Keyword: fruit
      Theme_Keyword: flower
      Theme_Keyword: insect
      Theme_Keyword: physical analysis
      Theme_Keyword: sugar content
      Theme_Keyword: mineral content
      Theme_Keyword: inorganic content
      Theme_Keyword: morphology
      Theme_Keyword: water content
      Theme_Keyword: calories
      Theme_Keyword: caloric value
      Theme_Keyword: proteins
      Theme_Keyword: fatty acids
      Theme_Keyword: database
      Theme_Keyword: physical analysis
      Theme_Keyword: bird population
    Place:
      Place_Keyword_Thesaurus: None
      Place_Keyword: Texas
      Place_Keyword: Louisiana
      Place_Keyword: Alabama
      Place_Keyword: Mississippi
      Place_Keyword: Southeastern United States
      Place_Keyword: Southcentral United States
  Access_Constraints:
    This data will be available for public use after it has been published in
    peer-reviewed, scientific outlets.
  Use_Constraints:
    Acknowledgement of the National Wetlands Research Center as a data source
    would be appreciated in products developed from these data.  Users should
    be aware of that comparisons with other data sets for the same area from
    other time periods may be inaccurate due to differences in data collection
    and lab analyses procedures.
  Point_of_Contact:
    Contact_Information:
      Contact_Organization_Primary:
        Contact_Organization: U.S. Geological Survey, National Wetlands Research Center
        Contact_Person: Clint Jeske/Wylie Barrow
      Contact_Position: Biologists
      Contact_Address:
        Address_Type: Mailing and Physical
        Address: 700 Cajundome Blvd
        City: Lafayette
        State_or_Province: LA
        Postal_Code: 70506
        Country: USA
      Contact_Voice_Telephone: 337 266-8500
      Contact_Facsimile_Telephone: 337 266-8513
      Contact_Electronic_Mail_Address: clint_jeske@usgs.gov or wylie_barrow@usgs.gov
  Data_Set_Credit:
    The USGS National Wetlands Research Center would like to acknowledge the
    US Fish and Wildlife Service for project funding.
  Native_Data_Set_Environment: Microsoft Excel
  Analytical_Tool:
    Analytical_Tool_Description:
      The HPLC (High Peformance Liquid Chromatography) Perkin Elmer LC Pump
      250 Model and the Series 200 Refractive Index Detector - to measure
      sugar content.
    Tool_Access_Information:
      Online_Linkage: http://las.perkinelmer.com
      Tool_Access_Instructions: Contact manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Perkin Elmer
        Contact_Address:
          Address_Type: Physical
          Address: 549 Albany Street
          City: Boston
          State_or_Province: MA
          Postal_Code: 02118-2512
          Country: USA
        Contact_Voice_Telephone: 800 762-4000
        Contact_Facsimile_Telephone: 203 944-4902
        Contact_Electronic_Mail_Address: productinfo@perkinelmer.com
  Analytical_Tool:
    Analytical_Tool_Description:
      The Bomb Calorimeter, Model Parr 1261 - measure the number of calories
      per gram
    Tool_Access_Information:
      Online_Linkage: http://www.parrinst.com
      Tool_Access_Instructions: See manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Parr Instrument Company
        Contact_Address:
          Address_Type: Physical
          Address: 211 Fifty Tird Street
          City: Moline
          State_or_Province: IL
          Postal_Code: 61265-9984
          Country: USA
        Contact_Voice_Telephone: 800 872-7720
        Contact_Facsimile_Telephone: 309 762-9453
        Contact_Electronic_Mail_Address: parr@parrinst.com
  Analytical_Tool:
    Analytical_Tool_Description:
      The ICP (Inductively Coupled Plasma) Perkin Elmer Optima 3000 - to
      measure mineral content
    Tool_Access_Information:
      Online_Linkage: http://las.perkinelmer.com
      Tool_Access_Instructions: See manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Perkin Elmer
        Contact_Address:
          Address_Type: Physical
          Address: 549 Albany Street
          City: Boston
          State_or_Province: MA
          Postal_Code: 02118-2512
          Country: USA
        Contact_Voice_Telephone: 800 762-4000
        Contact_Facsimile_Telephone: 203 944-4902
        Contact_Electronic_Mail_Address: productinfo@perkinelmer.com
  Analytical_Tool:
    Analytical_Tool_Description:
      The Lyphilizer, Labconco Lyphlock 12 - to dry out samples in
      preparation for analyses
    Tool_Access_Information:
      Online_Linkage: http://www.labconco.com
      Tool_Access_Instructions: See manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Labconco
        Contact_Address:
          Address_Type: Physical
          Address: 8811 Prospect Avenue
          City: Kansas City
          State_or_Province: MO
          Postal_Code: 64132-2696
          Country: USA
        Contact_Voice_Telephone: 800 821-5525
        Contact_Facsimile_Telephone: 816 363-0130
        Contact_Electronic_Mail_Address: labconco@labconco.com
  Analytical_Tool:
    Analytical_Tool_Description:
      The Microwave Digestion System, CEM MDS-2100 - to digest acids in
      preparation for ICP analyses
    Tool_Access_Information:
      Online_Linkage: http://www.cem.com
      Tool_Access_Instructions: Contact manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: CEM Corporation
        Contact_Address:
          Address_Type: Mailing
          Address: P.O. Box 200
          City: Matthews
          State_or_Province: NC
          Postal_Code: 28106-0200
          Country: USA
        Contact_Voice_Telephone: 800 726-3331
        Contact_Facsimile_Telephone: 704 821-7894
        Contact_Electronic_Mail_Address: info@cem.com
  Analytical_Tool:
    Analytical_Tool_Description:
      The Muffle Furnace, Thermolne Furnace 30400 - to ash samples in
      preparation of measuring inorganic content
    Tool_Access_Information:
      Online_Linkage: http://www.barnsteadthermolyne.com
      Tool_Access_Instructions: contact manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Barnstead International
        Contact_Address:
          Address_Type: Physical
          Address: 2555 Kerper Blvd
          City: Dubuque
          State_or_Province: IO
          Postal_Code: 52001-1478
          Country: USA
        Contact_Voice_Telephone: 800 446-6060
        Contact_Facsimile_Telephone: 563 589-0516
        Contact_Electronic_Mail_Address: mkt@barnsteadthermolyne.com
  Analytical_Tool:
    Analytical_Tool_Description: Soxtex System HT 1043 - to extract total lipids
    Tool_Access_Information:
      Online_Linkage: http://www.foss.dk
      Tool_Access_Instructions: contact manufacturer for specific access information
    Tool_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: FOSS A/S Corporation
        Contact_Address:
          Address_Type: Physical
          Address: 7682 Executive Drive
          City: Eden Prairie
          State_or_Province: MN
          Postal_Code: 55344
          Country: USA
        Contact_Voice_Telephone: 952 974-9892
        Contact_Facsimile_Telephone: 952 974-9823
        Contact_Electronic_Mail_Address: info@fossnorthamerica.com

Data_Quality_Information:
  Attribute_Accuracy:
    Attribute_Accuracy_Report:
      The accuracy of the attributes measured or calculated is based on the
      accuracy of the equipment used, calibrations performed, measurements
      recorded, statistical analysis conducted, and element of human error
      involved. For caloric analysis, standard benzoic acid tablets were run
      to ensure a constant coefficient. For mineral content analysis,
      strontium or yiterbium were the internal standards for the project.
      For sugar content, a standard of sucrose, glucose, and fructose is run
      before analyzing samples. If the pressure is greater than 1250 psi or
      lower than 900 psi, the instrument is checked and maintained.
  Logical_Consistency_Report:
    Samples are prepared within three days of receipt and processed according
    to developed methodology (see methodology below). Once analyses are
    complete, an inventory of samples are maintained and all sugars are
    frozen.
  Completeness_Report:
    Not all samples were analyzed; fruits that were not ripe were not
    considered for analyses. For caloric value, a total of ten runs were
    performed for most plant species (5 pulp, 5 whole) with fruits; this
    excludes toothache, blackberry and mulberry, where only five whole samples
    are run. Also, a pellet press was not used for all species bombed.
  Lineage:
    Methodology:
      Methodology_Type: Field
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: none
        Methodology_Keyword: label
        Methodology_Keyword: bagged
        Methodology_Keyword: ice
        Methodology_Keyword: plastic bag
      Methodology_Description:
        Field collection - the date when and area where fruits and flowers
        were collected are noted. Fruits are placed in a plastic bag
        separately and put on ice until returning to the lab. If possible,
        35mm photos are taken of the specimen for an image database.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: none
        Methodology_Keyword: Distilled water
        Methodology_Keyword: DI water
        Methodology_Keyword: air dried
        Methodology_Keyword: measurements
        Methodology_Keyword: calipers
        Methodology_Keyword: lyophilizer
        Methodology_Keyword: dried
        Methodology_Keyword: mass
        Methodology_Keyword: random
        Methodology_Keyword: pulp
        Methodology_Keyword: sieve
        Methodology_Keyword: forceps
        Methodology_Keyword: probe
        Methodology_Keyword: heating
        Methodology_Keyword: ultra cold freezer
        Methodology_Keyword: weight
      Methodology_Description:
        Physical analysis - All fruits are rinsed with deionized water and
        allowed to air dry prior to analysis. Size and weight measurements
        are taken from fresh samples. Using calipers, two or more size
        measurements are recorded based on the shape of the fruit (two
        dimensions = spherical, oval, or cylindrical and three dimensions
        = other (drawing and description)). Height is measured from the
        underside of fruit to place of attachment to plant. Length and
        width are measured; length is considered larger than width and
        width, vice versa. After the mass and dimensions of ten randomly
        chosen fruits are recorded, the whole fruit is lyophilized.
        Percent water for the whole fruit is calculated as total mass of
        dried fruit divided by total mass of fresh fruit and multiplied by
        one hundred. The percent water of the pulp is also calculated; the
        mass of 10 whole fresh fruits is recorded. Pulp is removed and the
        mass recorded. Pulp can be removed three different ways. For most
        fruits, pulp is removed by rubbing dried fruit onto a sieve to
        grate the pulp. Removal occurs before drying for some fruits with
        the use of gloves and/or a probe and forceps. Pulp is removed
        differently for tallow because of its waxy coat. After heating the
        tallow fruit in warm water, the coat can be removed from the seeds
        by scraping with a probe and forceps. Methods for removal are
        chosen on which method will be most efficient without losing the
        sample.  After thoroughly dried, the pulp is removed from the
        lyophilizer and the mass recorded. The percent water for the pulp
        is calculated by dividing the mass of the fresh pulp by the mass
        of dried pulp, and multiplied by one hundred. For percent pulp,
        another ten fresh fruits are randomly picked. Weight measurements
        are taken, and the seeds are extracted by hand and then weighed.
        The percent pulp is determined by subtracting the seed mass from
        whole fruit mass, and then divided by whole fruit mass. All drying
        occurs in the lyophilizer for a minimun of 48 hours. Dried samples
        are stored in an ultra-cold freezer at a constant temperature of
        -80 degrees Celsius.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: None
        Methodology_Keyword: bomb calorimeter
        Methodology_Keyword: energy content
        Methodology_Keyword: calorie
        Methodology_Keyword: mortar
        Methodology_Keyword: pestle
        Methodology_Keyword: hot plate
        Methodology_Keyword: DI water
        Methodology_Keyword: benzoic acid tablets
        Methodology_Keyword: caloric value
        Methodology_Keyword: pellet press
      Methodology_Description:
        Bomb Calorimetry - The bomb calorimeter is used to measure the
        number of calories per gram of each dried sample. Energy content
        is measured for whole fruit, flowers and pulp when applicable. A
        total of ten runs were conducted on plant species with fruits (5
        pulp, 5 whole) and five or ten runs were performed for flowering
        species (5 male, 5 female or 5 complete). The use of a pellet
        press reduced the possibility of incomplete burns. The dried
        samples were crushed with a mortar and pestle. A sample size for
        the bomb calorimeter is in the range of 0.3 to 0.8 grams dry mass.
        Oxygen pressure is set at 450 psi. A hot plate is used to heat 2
        lites of deionized water to 35 degrees Celsius, which is poured
        into a bucket inside the calorimeter. The change in temperature in
        degrees Celsius is taken from the calorimeter to calculate caloric
        content. The change in temp multiplied by the coefficient and
        dividied by mass of sample in grams calculates the calorie/gram.
        The coeffient is determined by running three standard benzoic acid
        tablets. The caloric value of each tablet is predetermined, and
        the coeffient is determined by back calculations.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: None
        Methodology_Keyword: ashing
        Methodology_Keyword: inorganic matter
        Methodology_Keyword: crucible
        Methodology_Keyword: dessicator
        Methodology_Keyword: temperature
        Methodology_Keyword: percent
        Methodology_Keyword: ashed
        Methodology_Keyword: weight
      Methodology_Description:
        Ashing - Ashing a sample will give the percent of inorganic matter
        of the sample. Dried pulp and whole fruits and flowers are ashed.
        Each sample consists of more than one crushed fruit for each
        species, and each sample has a minimum dry mass of 0.25 grams. A
        crucible is cleaned with DI water and placed in the oven for four
        hours at 450 degrees Celsius. The crucible is allowed to cool to
        room temperature in a dessicator for 10 to 20 minutes before
        weighing. The weight of the crucible is measured and the sample is
        added; the total weight of the sample and crucible is measured and
        recorded. The oven is programmed according to standard operating
        procedures with rate 1 = 25C, temp 1 = 450C, time 1 = 4.0hours,
        rate 2= 40C, hold = 100C, and time 2 = 20hours. Rate is the
        increment at which temperature will increase or decrease. Hold is
        activated in order to extend the time that the oven will remain at
        100C. The crucible is then removed once the oven has returned to a
        temperature of 100C; it is then placed in a dessicator and allowed
        to cool to room temperature for 10 to 20 minutes. The mass of the
        crucible and ash is recorded and the percent ash calculated as ash
        (g) divided by sample (g) multipled by one hundred.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: None
        Methodology_Keyword: mineral content
        Methodology_Keyword: ICP
        Methodology_Keyword: Atomic Emissions Spectroscopy
        Methodology_Keyword: AES
        Methodology_Keyword: Inductively Coupled Plasma
        Methodology_Keyword: crucible
        Methodology_Keyword: HCl acid
        Methodology_Keyword: vials
        Methodology_Keyword: refrigerator
        Methodology_Keyword: Aluminum
        Methodology_Keyword: Barium
        Methodology_Keyword: Calcium
        Methodology_Keyword: Copper
        Methodology_Keyword: Iron
        Methodology_Keyword: Potassium
        Methodology_Keyword: Magnesium
        Methodology_Keyword: Manganese
        Methodology_Keyword: Sodium
        Methodology_Keyword: Nickel
        Methodology_Keyword: Phosphorus
        Methodology_Keyword: Lead
        Methodology_Keyword: Tin
        Methodology_Keyword: Zinc
        Methodology_Keyword: Strontium
        Methodology_Keyword: Yiterbium
      Methodology_Description:
        Mineral content - Ashed material is dissolved and removed from the
        crucibles by applying 3ml of HCl acid and diluting solution to
        25ml using deionized water. The solution is placed into 50 ml
        vials for storage and stored in a refrigerator until ICP-AES
        analysis can be conducted. Samples are shaked well prior to
        analysis to distribute heavy elements. The Optima 2000 software is
        specified for a number of elements (Al, Ba, Ca, Cu, Fe, K, Mg, Mn,
        Na, Ni, P, Pb, Si, and Zn). Strontium or Yiterbium are the
        internal standards used for this protocol. Two samples of whole
        and pulp for each species are analyzed by the ICP. Each sample is
        analyzed three times and the average concentration for each
        element specified is available in the output data.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: None
        Methodology_Keyword: HPLC
        Methodology_Keyword: High Performance Liquid Chromatography
        Methodology_Keyword: chromatographic process
        Methodology_Keyword: mass transfer
        Methodology_Keyword: stationary
        Methodology_Keyword: mobile
        Methodology_Keyword: fatty acid analysis
        Methodology_Keyword: lipid analysis
        Methodology_Keyword: liquid nitrogen
        Methodology_Keyword: filtered
        Methodology_Keyword: proteins
        Methodology_Keyword: Lowery's reagent
        Methodology_Keyword: sugars
        Methodology_Keyword: standard
        Methodology_Keyword: test tubes
        Methodology_Keyword: Vortex
        Methodology_Keyword: Folin reagent
        Methodology_Keyword: incubation
      Methodology_Description:
        HPLC capabilities - Chromatographic processes can be defined as
        separation techniques involving mass-transfer between stationary
        and mobile phases. Compounds will not be isolated for analysis;
        some samples may be purified, concentrated, or diluted. For
        sugars, samples are prepared by exposing dried pulp or whole fruit
        to liquid nitrogen to form a powder. A known amount of this powder
        is dissolved and diluted with a known amount of water. The
        solution is then passed through a fine filter. The filtered
        solution is introduced into the column on the HPLC. It takes about
        fifteen minutes to see an output. Outputs are compared to other
        standards run previously that day and previous days to determine
        identities and concentrations of individual sugars. For proteins,
        the samples are prepared using the same nitrogen powdering
        procedure described in the sugar analysis. A known amount of the
        sample is tested for total protein using the Lowery's protein
        estimation assay. One method is to transfer sample containing up
        to 40 micrograms of protein to test tubes and bring volume up to
        200 microliters with water. Add 1 ml of mixture and mix will with
        the Vortex. Incubate for 10 minutes at room temperature. Add 50
        microliters of folin reagent, mix well with Vortex. Incubate for
        30 minutes at room temperature. Read at 540 nm against an H20
        blank.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present
  Lineage:
    Methodology:
      Methodology_Type: Lab
      Methodology_Identifier:
        Methodology_Keyword_Thesaurus: None
        Methodology_Keyword: lipid
        Methodology_Keyword: total lipids
        Methodology_Keyword: plug
        Methodology_Keyword: magnetic cuffs
        Methodology_Keyword: columns
        Methodology_Keyword: evaporate
        Methodology_Keyword: cellulose thimble
        Methodology_Keyword: pet ether
        Methodology_Keyword: teflon boiling chip
        Methodology_Keyword: chloroform
        Methodology_Keyword: glass wool
        Methodology_Keyword: elute
        Methodology_Keyword: hexane
      Methodology_Description:
        Lipid analysis - For separating lipid classes, a C18 SPE column is
        used. The column is washed with hexane. The lipids are dissolved
        in hexane isopropanol3:2 and loaded into the column. Netural
        lipids are eluted with the hexan-isopropanol3:2, glycolipids and
        ceramids in acetone-methanol9:1, and phospholipids in methanol.
        For Soxhlet extraction for total lipids, a Soxtex System HT 1043
        is used. Cellulose thimbles are cleaned and fitted with glass wool
        (as plugs) into magnetic cuffs and metal cylinders. 2-3 grams of
        crushed and freeze-dried samples are placed into the thimbles. The
        rack of thimbles is positioned in the slot below the extraction
        column. The cuffs and the thimbles are then drawn into the
        extraction columns. Six numbered, cleaned and dried metal boiling
        cups are placed into the handled cup rack; telfon boiling chips
        are added, and then 50 ml of pet ether into each cup. Cold water
        is then turned on in the extraction column. The prepared cups are
        placed on the heating plate under the columns. The cups are then
        positioned to seal them to the teflon rings at the columns'
        bottoms. The samples are boiled for five hours; the evaporation
        process is then started. The cups are checked every fifteen
        minutes until lipids are extracted or the samples are completely
        dried. The cups are removed and weighed.
    Process_Step:
      Process_Description: See methodology
      Process_Date: 2003-present

Entity_and_Attribute_Information:
  Overview_Description:
    Entity_and_Attribute_Overview:
      The database consists of parameters obtained through different
      analyses. Physical parameters are measured and include size and weight
      measurements, percent water of the whole fruit, and precent water of
      the pulp. chemical parameters are measured through different types of
      analyses. Caloric value of samples is measured in calorie/grams. The
      inorganic content is measured in percentage and is obtained by
      dividing ash(g)/sample(g) and multiplying by 100. The mineral content
      is measured in concentrations of mg/ml and is calculated by the ICP.
      The sugars parameter measures the number of sugars present in the
      sample and is measured in concentration of parts per million. The
      proteins parameter is measured in concentration of total amount
      detected in the sample.
    Entity_and_Attribute_Detail_Citation: USGS National Wetlands Research Center

Metadata_Reference_Information:
  Metadata_Date: 20040127
  Metadata_Review_Date: 200402
  Metadata_Contact:
    Contact_Information:
      Contact_Organization_Primary:
        Contact_Organization: US Geological Survey, National Wetlands Research Center
      Contact_Position: metadata specialist
      Contact_Address:
        Address_Type: Mailing and Physical
        Address: 700 Cajundome Blvd
        City: Lafayette
        State_or_Province: LA
        Postal_Code: 70506
        Country: USA
      Contact_Voice_Telephone: 337 266-8500
      Contact_Facsimile_Telephone: 337 266-8513
  Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata
  Metadata_Standard_Version: FGDC-STD-001-1998
  Metadata_Extensions:
    Profile_Name:
      Biological Data Profile of Content Standard for Digital Geospatial
      Metadata
    Online_Linkage: http://www.fgdc.gov/standards/status/sub5_2.html
  Metadata_Access_Constraints: None
  Metadata_Use_Constraints:
    Acknowledgement of the National Wetlands Research Center as the metadata
    source would be appreciated. Please cite the original metadata when using
    portions to create another record for slightly altered data, such as
    reprojection.