5traptonyrln effeota od chlorophyll in higher plrinta.
                Nrrnay Zlebur, 1949-1950
                                              r
  PrtivRaoll, Hutner and Sahate (Proo. Bee. Exp, Blol. & Med.
1948, 69:219-282) showed that o ertiiin ratrnins of &Elena
in the protaenee of streptomyoln (SVM) undergo IZ permanent yg+
chlorophyll but all1 aontlnue to groa well if H. proper energy aourcze
la supplied.  Their paper KLEO refer6 to $everfA of von Euler and
Brcraao who rnported that asoda germlruated in ST% aolutlona developed
Into o hloronhyll. daflslent rileedllnge -  no report beyond the seedling
s tar?e 6

A, Methods.

1. Btreptomysln used in two forma:
   Dlhydroatreptomyaln, 1.2 go of whlrh 1s equlvfilcnt to
     1 go STId base.

  The reaetlone to the two forms were lndlstlngulsht~hla.
The doses given will be referred to in terrao of STM base,
2. Seed treatmenta

  IJr?sd AgHr plates with .S$ rtgaro  STM dlaRolved In sterile
   water and f~flded to the sterile n~irm itgiir,  No 8olltFi?l~ nation
    with thla meWoda
3* Bud trentmentn

   Agar caf~ps raade In the following fashion:  Take about 15 IWR. of
  a Jumb(b drinking atraw (6 EWE* dlam.), @over one end with
   {L fmmx aquars of Alulalnum foil, wind A 5mn. wide strip of
   aeotah tnpa around tightly brlnglng the, two ctieky sideS to-
   gether where the ends meet. Thla gives R tab, R slit in whlsh
   lo ureful to hold H thitead from allpplnp; up or down In *Rae
  It is neueas&ry to tie the str&w to the plant, A gal of .?$
  nnd 1% 8TM la used,  with .3 ml. In e;Lrh Htrtsw.

   Ltinolln suspensions were triad but didn't rork well..
4* Xn,Jecptlona were mW%? with non-sterllj!e golutlons of vcirylng
  strenflhs,

Be Experiments.
1, RRdiahel  - 36arlet Globe
   be Seed treatments.

  Seeds aterllis/ed with HgCl 2:lOOO and deturgent (together),
   rlneed, soaked overnight en sterile water,  reaterilised,
   Pleaed on agar plates,  Aftor two dnys transfened to test
  tubes with .?$ figar, 2% mmroae, and Randolph and Cox
    mlnelr&la.  Bone seedlings entirely white, Borne pfirtly green,
   Growth poorer than rontrolla and exprame&y vnrlr:ble. Even
   If aer~~7.1 parta of the treated seedlings remcain grean, they
   do not Qurvlve In raoil,  On su ttr medium seedZIngs never
   grew more thnn about 6 inches 7 however,  csont-olrr, also Aid
   poorly In sterile &Ltureo  Attempts at quirntitritlve eom-
   p~ri~ons of modish growth were AbAndoned bea:.use oi the v~lr-
   l*~blllty of the rar%terlrtla
b, J3u(L trei%tnent
  Inflo#reeaenaes or gro\-Ang tips were trented by aquaacing
   them into RgFrr filled IttrHws, hrtving removed aa mriny buda
   or le~vas NB neeessriry to get R good fit.  Not nt?aessRry
   to tie strano on.  Qtrr:wg left on about 5 days or untll afrfar
    hrtd dried.  The lertves nnd stems Inside were InvnrWbly


   blpaehed when they exprinded, with some green remnlning IZ the
   lg~ves weye quite large when treirted, The effesats could ~180
    be oeen on le~veo farBEer down on the stem,  white lmvsa wrere
   often Htlff, and r~lw~ys grew more rsloalg then green onea.
    Affected atena were redder thr+n norqrrl.  men the rzpio~l grow-
   lng point wna treatc?d, green aide Rhoota farther down began
   mp.ld growth and aosounted for aost of the further growth of
    the plant,  Flowerer arlslng from treated buds freq!jentlg nborted
   beTore openlng.  Borne opened into flopera snnller n~cl ntnker
    th;Ln noran?-,  Others qpeared norm&  Pollen from the ?:xat two
   types nrls ap!?lled to normiLl etlgmam and about SO$ set aeed.
   Individu~il flower bags wepl`e used. No rerrl aontrol for fer-
   tillty comp~rlsone wpi8 used, but potlen from one lnflo#res-
   sense tbt ww only very slightly pale effected lOO$ fertlll-
    cstione  It seems likely that pol'len fertlllt;P la aotunlly
   reilucleii by severe 3W treatment.  Treated flowers were lrapssc
   pollinated with normril poll6n, but of 25-50 flowers only
  2 showed the beggwlngs of fruit development. One of theee
   aborted, and the other produced two ahrlveled seeds, Female
   taterlllty le unqueatlon&bly present.  Horaar\l g x ST@ a7 and
   csomprirable normal x normihl seed@ nere plentlftul and should
   be groe;n out.
2, Peas - Alaska - Bud treatment
Slmllnr to th,it for raillshes except thttt the tetmws hr;.d to be
  tied on,  Several afferted :Jods started growth, preeuntibly self-
pollinrited, but txborted after ranrshlng A m~xlraum slz e of about
3/4 lnoh long.  Pollen output of affelted flowers sserued 7oFe re-
duaect thtin In the @?:ae of rndlslihes; the few attam@bs to use It
for fe~tlllzlng normi. flowers were uneureeeerful.
3. Tomntoes - Bonny Best
H. Bud. trerktment.  Grovlng points were killed bv AS short
  A tretitment ff.9 1 day with 1 $ S'i%id.  MO chlorophyll abnormalltie8
   frppellred, altho the plant8 grel' sowenhtrt pu~ll$h.tuu~ pb')H
b. Injeetlon.  1% or .lfi 3TM lao'-utlons were lnJs@Yed in%0 enA8
   of o\rt-off aI& brrrncks,  No posltlwe ,effaet wcL8 showy?.
4. Corn
a. Inje~tlone.
  Sepc!llnge in $ol18Rbout 3-4 fnlshas high 1nJeateA from the top
  wlth a ra&ttlon eontdilnlngalfl STM and .O$$ deturger.t,  %eedllnga
  k~aa~ beanrae bleaahed,and died in &out 3 week& Controls ln-
   jaated with deturgent only vere ok,
b. Seed treatments,  3ame method@ as for mdished, :ifter 8 deys on
   STM agar et111 no effect,
o. Embryo treatments.
  Seeda eterlliesd, asked 2 daya  embryoa exrlsed and reloterll-
  leed, then pltreed on STka tqg*tr &tes. Efiecrt ele~r after 5
   dR$a and seedlings uould be tr~~ns#!bsaed to test tambaa as with
   rctdlehee,  QuAntltatfve testa in light Rnd dark were also
   run to Mxrmlne whtsther she sfieeted plants aan utilize mtrosee