LOCUS       NC_005816               9609 bp    DNA     circular BCT 21-JUL-2008
DEFINITION  Yersinia pestis biovar Microtus str. 91001 plasmid pPCP1, complete
            sequence.
ACCESSION   NC_005816
VERSION     NC_005816.1  GI:45478711
PROJECT     GenomeProject:10638
DBLINK      Project:10638
KEYWORDS    .
SOURCE      Yersinia pestis biovar Microtus str. 91001
  ORGANISM  Yersinia pestis biovar Microtus str. 91001
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Yersinia.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Zhou,D., Tong,Z., Song,Y., Han,Y., Pei,D., Pang,X., Zhai,J., Li,M.,
            Cui,B., Qi,Z., Jin,L., Dai,R., Du,Z., Wang,J., Guo,Z., Wang,J.,
            Huang,P. and Yang,R.
  TITLE     Genetics of metabolic variations between Yersinia pestis biovars
            and the proposal of a new biovar, microtus
  JOURNAL   J. Bacteriol. 186 (15), 5147-5152 (2004)
   PUBMED   15262951
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   Song,Y., Tong,Z., Wang,J., Wang,L., Guo,Z., Han,Y., Zhang,J.,
            Pei,D., Zhou,D., Qin,H., Pang,X., Han,Y., Zhai,J., Li,M., Cui,B.,
            Qi,Z., Jin,L., Dai,R., Chen,F., Li,S., Ye,C., Du,Z., Lin,W.,
            Wang,J., Yu,J., Yang,H., Wang,J., Huang,P. and Yang,R.
  TITLE     Complete genome sequence of Yersinia pestis strain 91001, an
            isolate avirulent to humans
  JOURNAL   DNA Res. 11 (3), 179-197 (2004)
   PUBMED   15368893
REFERENCE   3  (bases 1 to 9609)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2004) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   4  (bases 1 to 9609)
  AUTHORS   Song,Y., Tong,Z., Wang,L., Han,Y., Zhang,J., Pei,D., Wang,J.,
            Zhou,D., Han,Y., Pang,X., Zhai,J., Chen,F., Qin,H., Wang,J., Li,S.,
            Guo,Z., Ye,C., Du,Z., Lin,W., Wang,J., Yu,J., Yang,H., Wang,J.,
            Huang,P. and Yang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2003) The Institute of Microbiology and
            Epidemiology, Academy of Military Medical Sciences, No. 20,
            Dongdajie Street, Fengtai District, Beijing 100071, People's
            Republic of China
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AE017046.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Yersinia pestis biovar Microtus str. 91001"
                     /mol_type="genomic DNA"
                     /strain="91001"
                     /db_xref="taxon:229193"
                     /plasmid="pPCP1"
                     /biovar="Microtus"
     repeat_region   1..1954
                     /mobile_element="insertion sequence:IS100"
     gene            87..1109
                     /locus_tag="YP_pPCP01"
                     /db_xref="GeneID:2767718"
     CDS             87..1109
                     /locus_tag="YP_pPCP01"
                     /note="similar to corresponding CDS from previously
                     sequenced pPCP plasmid of Yersinia pestis KIM (AF053945)
                     and CO92 (AL109969), also many transposase entries for
                     insertion sequence IS100 of Yersinia pestis. Contains
                     IS21-like element transposase, HTH domain
                     (Interpro|IPR007101)"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transposase"
                     /protein_id="NP_995567.1"
                     /db_xref="GI:45478712"
                     /db_xref="GeneID:2767718"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1106..1888
                     /locus_tag="YP_pPCP02"
                     /db_xref="GeneID:2767716"
     CDS             1106..1888
                     /locus_tag="YP_pPCP02"
                     /note="similar to corresponding CDS form previously
                     sequenced pPCP plasmid of Yersinia pestis KIM (AF053945)
                     and CO92 (AL109969), also many ATP-binding protein entries
                     for insertion sequence IS100 of Yersinia pestis. Contains
                     Chaperonin clpA/B (Interpro|IPR001270). Contains
                     ATP/GTP-binding site motif A (P-loop) (Interpro|IPR001687,
                     Molecular Function: ATP binding (GO:0005524)). Contains
                     Bacterial chromosomal replication initiator protein, DnaA
                     (Interpro|IPR001957, Molecular Function: DNA binding
                     (GO:0003677), Molecular Function: DNA replication origin
                     binding (GO:0003688), Molecular Function: ATP binding
                     (GO:0005524), Biological Process: DNA replication
                     initiation (GO:0006270), Biological Process: regulation of
                     DNA replication (GO:0006275)). Contains AAA ATPase
                     (Interpro|IPR003593, Molecular Function: nucleotide
                     binding (GO:0000166))"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase/IS protein"
                     /protein_id="NP_995568.1"
                     /db_xref="GI:45478713"
                     /db_xref="GeneID:2767716"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            2925..3119
                     /gene="rop"
                     /locus_tag="YP_pPCP03"
                     /gene_synonym="rom"
                     /db_xref="GeneID:2767717"
     CDS             2925..3119
                     /gene="rop"
                     /locus_tag="YP_pPCP03"
                     /gene_synonym="rom"
                     /note="Best Blastp hit =gi|16082682|ref|NP_395229.1|
                     (NC_003132) putative replication regulatory protein
                     [Yersinia pestis], gi|5763813|emb|CAB531 66.1| (AL109969)
                     putative replication regulatory protein [Yersinia pestis];
                     similar to gb|AAK91579.1| (AY048853), RNAI modulator
                     protein Rom [Salmonella choleraesuis], Contains Regulatory
                     protein Rop (Interpro|IPR000769)"
                     /codon_start=1
                     /transl_table=11
                     /product="putative replication regulatory protein"
                     /protein_id="NP_995569.1"
                     /db_xref="GI:45478714"
                     /db_xref="GeneID:2767717"
                     /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQARFEAESETGT"
     gene            3486..3857
                     /locus_tag="YP_pPCP04"
                     /db_xref="GeneID:2767720"
     CDS             3486..3857
                     /locus_tag="YP_pPCP04"
                     /note="Best Blastp hit = gi|321919|pir||JQ1541
                     hypothetical 16.9K protein - Salmonella typhi murium
                     plasmid NTP16."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_995570.1"
                     /db_xref="GI:45478715"
                     /db_xref="GeneID:2767720"
                     /translation="MSKKRRPQKRPRRRRFFHRLRPPDEHHKNRRSSQRWRNPTGLKD
                     TRRFPPEAPSCALLFRPCRLPDTSPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCT
                     NPPFSPTTAPYPVTIVLSPTR"
     misc_feature    3498..3626
                     /locus_tag="YP_pPCP04"
                     /note="ProfileScan match to entry PS50323 ARG_RICH,
                     E-value 8.981"
     gene            4343..4780
                     /gene="pim"
                     /locus_tag="YP_pPCP05"
                     /db_xref="GeneID:2767712"
     CDS             4343..4780
                     /gene="pim"
                     /locus_tag="YP_pPCP05"
                     /note="similar to many previously sequenced pesticin
                     immunity protein entries of Yersinia pestis plasmid pPCP,
                     e.g. gi| 16082683|,ref|NP_395230.1| (NC_003132) ,
                     gi|1200166|emb|CAA90861.1| (Z54145 ) , gi|1488655|
                     emb|CAA63439.1| (X92856) , gi|2996219|gb|AAC62543.1|
                     (AF053945) , and gi|5763814|emb|CAB531 67.1| (AL109969)"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin immunity protein"
                     /protein_id="NP_995571.1"
                     /db_xref="GI:45478716"
                     /db_xref="GeneID:2767712"
                     /translation="MGGGMISKLFCLALIFLSSSGLAEKNTYTAKDILQNLELNTFGN
                     SLSHGIYGKQTTFKQTEFTNIKSNTKKHIALINKDNSWMISLKILGIKRDEYTVCFED
                     FSLIRPPTYVAIHPLLIKKVKSGNFIVVKEIKKSIPGCTVYYH"
     gene            complement(4815..5888)
                     /gene="pst"
                     /locus_tag="YP_pPCP06"
                     /db_xref="GeneID:2767721"
     CDS             complement(4815..5888)
                     /gene="pst"
                     /locus_tag="YP_pPCP06"
                     /note="Best Blastp hit =|16082684|ref|NP_395231.1|
                     (NC_003132) pesticin [Yersinia pestis],
                     gi|984824|gb|AAA75369.1| (U31974) pesticin [Yersinia
                     pestis], gi|1488654|emb|CAA63438.1| (X92856) pesticin
                     [Yersinia pestis], gi|2996220|gb|AAC62544.1| (AF053945)
                     pesticin [Yersinia pestis], gi|5763815|emb|CAB53168.1|
                     (AL1099 69) pesticin [Yersinia pestis]"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin"
                     /protein_id="NP_995572.1"
                     /db_xref="GI:45478717"
                     /db_xref="GeneID:2767721"
                     /translation="MSDTMVVNGSGGVPAFLFSGSTLSSYRPNFEANSITIALPHYVD
                     LPGRSNFKLMYIMGFPIDTEMEKDSEYSNKIRQESKISKTEGTVSYEQKITVETGQEK
                     DGVKVYRVMVLEGTIAESIEHLDKKENEDILNNNRNRIVLADNTVINFDNISQLKEFL
                     RRSVNIVDHDIFSSNGFEGFNPTSHFPSNPSSDYFNSTGVTFGSGVDLGQRSKQDLLN
                     DGVPQYIADRLDGYYMLRGKEAYDKVRTAPLTLSDNEAHLLSNIYIDKFSHKIEGLFN
                     DANIGLRFSDLPLRTRTALVSIGYQKGFKLSRTAPTVWNKVIAKDWNGLVNAFNNIVD
                     GMSDRRKREGALVQKDIDSGLLK"
     variation       5910..5911
                     /note="compared to AF053945"
                     /replace=""
     variation       5933^5934
                     /note="compared to AL109969"
                     /replace="a"
     variation       5933^5934
                     /note="compared to AF053945"
                     /replace="aa"
     variation       5948
                     /note="compared to AL109969"
                     /replace="c"
     gene            6005..6421
                     /locus_tag="YP_pPCP07"
                     /db_xref="GeneID:2767719"
     CDS             6005..6421
                     /locus_tag="YP_pPCP07"
                     /note="Best Blastp hit = gi|16082685|ref|NP_395232.1|
                     (NC_003132) hypothetical protein [Yersinia pestis],
                     gi|5763816|emb|CAB53169.1| (AL109969) hypothetical protein
                     [Yersinia pestis]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_995573.1"
                     /db_xref="GI:45478718"
                     /db_xref="GeneID:2767719"
                     /translation="MKFHFCDLNHSYKNQEGKIRSRKTAPGNIRKKQKGDNVSKTKSG
                     RHRLSKTDKRLLAALVVAGYEERTARDLIQKHVYTLTQADLRHLVSEISNGVGQSQAY
                     DAIYQARRIRLARKYLSGKKPEGVEPREGQEREDLP"
     variation       6525
                     /note="compared to AF053945 and AL109969"
                     /replace="c"
     gene            6664..7602
                     /gene="pla"
                     /locus_tag="YP_pPCP08"
                     /db_xref="GeneID:2767715"
     CDS             6664..7602
                     /gene="pla"
                     /locus_tag="YP_pPCP08"
                     /EC_number="3.4.23.48"
                     /note="outer membrane protease; involved in virulence in
                     many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in
                     Escherichia coli OmpT can degrade antimicrobial peptides;
                     in Yersinia Pla activates plasminogen during infection; in
                     Shigella flexneria SopA cleaves the autotransporter IcsA"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protease"
                     /protein_id="NP_995574.1"
                     /db_xref="GI:45478719"
                     /db_xref="GeneID:2767715"
                     /translation="MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLS
                     GKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNM
                     DDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFS
                     WTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSD
                     WVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGG
                     TQIIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF"
     gene            complement(7789..8088)
                     /locus_tag="YP_pPCP09"
                     /db_xref="GeneID:2767713"
     CDS             complement(7789..8088)
                     /locus_tag="YP_pPCP09"
                     /note="Best Blastp hit = gi|16082687|ref|NP_395234.1|
                     (NC_003132) putative transcriptional regulator [Yersinia
                     pestis], gi|5763818|emb|CAB53171.1| (AL109969) putative
                     transcriptional regulator [Yersinia pestis]."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transcriptional regulator"
                     /protein_id="NP_995575.1"
                     /db_xref="GI:45478720"
                     /db_xref="GeneID:2767713"
                     /translation="MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQK
                     QVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGRRVAFHV"
     gene            complement(8088..8360)
                     /locus_tag="YP_pPCP10"
                     /db_xref="GeneID:2767714"
     CDS             complement(8088..8360)
                     /locus_tag="YP_pPCP10"
                     /note="Best Blastp hit = gi|16082688|ref|NP_395235.1|
                     (NC_003132) hypothetical protein [ Yersinia pestis],
                     gi|5763819|emb|CAB53172.1| (AL109969) hypothetical protein
                     [Yersinia pestis]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_995576.1"
                     /db_xref="GI:45478721"
                     /db_xref="GeneID:2767714"
                     /translation="MADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRA
                     FYAFDPIRRAIVLCAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK"
     variation       8529^8530
                     /note="compared to AL109969"
                     /replace="tt"
ORIGIN      
        1 tgtaacgaac ggtgcaatag tgatccacac ccaacgcctg aaatcagatc cagggggtaa
       61 tctgctctcc tgattcagga gagtttatgg tcacttttga gacagttatg gaaattaaaa
      121 tcctgcacaa gcagggaatg agtagccggg cgattgccag agaactgggg atctcccgca
      181 ataccgttaa acgttatttg caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg
      241 ctgttgcttc actcctggat gaataccggg attatattcg tcaacgcatc gccgatgctc
      301 atccttacaa aatcccggca acggtaatcg ctcgcgagat cagagaccag ggatatcgtg
      361 gcggaatgac cattctcagg gcattcattc gttctctctc ggttcctcag gagcaggagc
      421 ctgccgttcg gttcgaaact gaacccggac gacagatgca ggttgactgg ggcactatgc
      481 gtaatggtcg ctcaccgctt cacgtgttcg ttgctgttct cggatacagc cgaatgctgt
      541 acatcgaatt cactgacaat atgcgttatg acacgctgga gacctgccat cgtaatgcgt
      601 tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa actgtggttc
      661 tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg tggcagttcg
      721 gcaaggagat gggcttctct ccccgactgt gtcgcccctt cagggcacag actaaaggta
      781 aggtggaacg gatggtgcag tacacccgta acagttttta catcccacta atgactcgcc
      841 tgcgcccgat ggggatcact gtcgatgttg aaacagccaa ccgccacggt ctgcgctggc
      901 tgcacgatgt cgctaaccaa cgaaagcatg aaacaatcca ggcccgtccc tgcgatcgct
      961 ggctcgaaga gcagcagtcc atgctggcac tgcctccgga gaaaaaagag tatgacgtgc
     1021 atcttgatga aaatctggtg aacttcgaca aacaccccct gcatcatcca ctctccatct
     1081 acgactcatt ctgcagagga gtggcgtgat gatggaactg caacatcaac gactgatggc
     1141 gctcgccggg cagttgcaac tggaaagcct tataagcgca gcgcctgcgc tgtcacaaca
     1201 ggcagtagac caggaatgga gttatatgga cttcctggag catctgcttc atgaagaaaa
     1261 actggcacgt catcaacgta aacaggcgat gtatacccga atggcagcct tcccggcggt
     1321 gaaaacgttc gaagagtatg acttcacatt cgccaccgga gcaccgcaga agcaactcca
     1381 gtcgttacgc tcactcagct tcatagaacg taatgaaaat atcgtattac tggggccatc
     1441 aggtgtgggg aaaacccatc tggcaatagc gatgggctat gaagcagtcc gtgcaggtat
     1501 caaagttcgc ttcacaacag cagcagatct gttacttcag ttatctacgg cacaacgtca
     1561 gggccgttat aaaacgacgc ttcagcgtgg agtaatggcc ccccgcctgc tcatcattga
     1621 tgaaataggc tatctgccgt tcagtcagga agaagcaaag ctgttcttcc aggtcatcgc
     1681 taaacgttac gaaaagagcg caatgatcct gacatccaat ctgccgttcg ggcagtggga
     1741 tcaaacgttc gccggtgatg cagcactgac ctcagcgatg ctggaccgta tcttacacca
     1801 ctcacatgtc gttcaaatca aaggagaaag ctatcgactc agacagaaac gaaaggccgg
     1861 ggttatagca gaagctaatc ctgagtaaaa cggtggatca atattgggcc gttggtggag
     1921 atataagtgg atcacttttc atccgtcgtt gacaccctga tgaattcacg tgttcacgcc
     1981 tgaataacaa gaatgccgga gatacgcagt catatttttt acacaattct ctaatcccga
     2041 caaggtcgta ggtcgttata ggaaaattct tagcaccatt ccggaacaat cagaacagca
     2101 ggccatgaac gactgacaac attacgaata taaaaaacgc acccgggcca gacattcccc
     2161 ctactgatta aaccagccgg acttgtccac ggaacggtct ttttaaaccg acacacagtc
     2221 tgagtacaga tacatgtcac gatgatgcag gattagcgga agagtgtgag cacgtttccg
     2281 ggaactgtgg tgaaccatag ctcaatattc gagtgagggc ataccggaaa cgcgctcaga
     2341 ttcgttgtaa cgcgattttc cgtaccgggc aattttttca gttgtttttt cgtttcatgt
     2401 cgtcagaaac gttctgagcg cgtttccggc atctgatgct acgcaaacca tccccatggt
     2461 cagttgacag ccggaaacac gcgggtgtcg ttttagcgta tcgacgggac ggcgtcgaga
     2521 agcacaaaaa acagatgttg tactcagtca gttgttttac agacagcact gcggcagatt
     2581 gaaaaagtac cgtactttca ggaatgtcca gaaaccatgt gtcagacttc gttctccccc
     2641 ttccgggtga atttttttgt catccgttca ggaatctctt tataacgatt actccatttc
     2701 aggatttttt atgtggcgtt tactacaggc aggatattca aaggcaaaaa aatcccccgg
     2761 aacaggcgga acccggacag ggggagaacg aatcgctaaa taattttcgt agttgtattt
     2821 cccatcgttg ctactgcaac gggatgaatt tgccgcagtt tatcctgtaa aacaatcctg
     2881 atttactcac actccacata tcactgacgg agcacaacgg aatagtgaac aaacaacaac
     2941 aaactgcgct gaatatggcg cgatttatca gaagccagag cctgatactg cttgaaaaac
     3001 tggatgctct ggatgccgac gagcaggcgg ccatgtgtga acgactgcac gaactcgcgg
     3061 aagaactcca gaacagcatc caggctcgct ttgaagccga aagtgaaaca ggaacataac
     3121 gaagctcccg gagacggtca cagcttgtct gtgaacggat gccgggagca gacaagcccg
     3181 tcagggcgcg tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag
     3241 cgatagcgga gtgtatactg gcttagtcat gcggcatcag tgcggattgt atgaaaagtg
     3301 caccatgtac ggtgtgaaat gccgcacaga tgcgtaagga gaacatgcag atgccgatgc
     3361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg
     3421 tctgctcact caaaagcggt gatactgtta tccacacaat caggggataa cgccggaaag
     3481 aacatgtgag caaaaaacga agaccccaga aaaggccgcg ccggaggcgc tttttccata
     3541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3601 cgacaggact taaagatacc aggcgtttcc ccccggaagc tccctcgtgc gctctcctgt
     3661 tccgaccctg ccgcttaccg gatacctctc cgcctttctc ccttcgggaa gcgtggcgct
     3721 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3781 ctgtgtgcac gaaccccccg ttcagcccga ccactgcgcc ttatccggta actatcgtct
     3841 tgagtccaac ccggtaagac acgactttac gccactggca gcagccattg gtaactgaaa
     3901 agtggattta gatacgcaga actcttgaag ttgaagcctt atcgcggcta cactgaaagg
     3961 acagcatttg gtatctgtgc tccacttaag ccagctacca caggttagaa agcctgagaa
     4021 acttctaacc ttcgaaagaa cccacgcctg agaacgtggg ttttttcgtt tacaggcagc
     4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaattgc
     4141 gctcccgatc agttcagcag aagattatga tggggttcta tgggtattgc tgcggtaaca
     4201 cccatgttac ttgaggttgt atgtagtctg tgtagaatta tacacataag gcttaaactg
     4261 ctcttttttt tcaatatgca attggaagtt cattgactac ataaatagat tattccaaat
     4321 aatttattta tgtaagaaca ggatgggagg gggaatgatc tcaaagttat tttgcttggc
     4381 tctcatattt ttatcatcaa gtggccttgc agaaaaaaac acatatacag caaaagacat
     4441 cttgcaaaac ctagaattaa atacctttgg caattcattg tctcatggca tctatgggaa
     4501 acagacaacc ttcaagcaaa ccgagtttac aaatattaaa agcaacacca aaaaacacat
     4561 tgcacttatc aataaagaca actcatggat gatatcatta aaaatactag gaattaagag
     4621 agatgagtat actgtctgtt ttgaagattt ctctctaata agaccgccaa catatgtagc
     4681 catacatcct ctacttataa aaaaagtaaa atctggaaac tttatagtag tgaaagaaat
     4741 aaagaaatct atccctggtt gcactgtata ttatcattaa tagcaagccc ctcattatta
     4801 tgaggggctc atggttattt taacaatcca ctatcgatat ctttttgcac cagagcgccc
     4861 tctcgtttac gtctgtcaga cattccatca acaatattat taaaagcatt tacaaggcca
     4921 ttccagtctt ttgcgataac tttattccat actgtgggag cagttctgga taacttaaac
     4981 cctttttgat atccaataga caccagtgct gtacgggttc tcaacggtaa atcgctgaac
     5041 cgaagaccga tattagcgtc attgaaaaga ccttcaatct tatgtgagaa tttatcaata
     5101 taaatattag ataagagatg agcttcatta tcagaaagcg tcagaggtgc tgttctcact
     5161 ttatcataag cctccttccc tcgaagcata taatacccat caagtctatc tgcaatatac
     5221 tgagggacac cgtcattcaa taaatcctgt ttgcttcgct gaccaaggtc aaccccggaa
     5281 ccgaatgtaa caccggtact gttaaaataa tcgctactag gattagacgg aaaatgactt
     5341 gtcggattaa acccttcaaa accattactg gagaaaatat cgtggtcaac aatatttacc
     5401 gaacgacgta aaaattcctt cagttgacta atattgtcaa agttaatgac agtgttgtcc
     5461 gctaggacga tgcgatttcg gttattattc agaatgtctt cgttctcttt cttatcgaga
     5521 tgttcaatag attcggcaat cgttccctca agaaccatga cacggtagac tttcacaccg
     5581 tctttttcct gacctgtttc aacagttatt ttctgttcgt aagacacggt cccttcagtt
     5641 tttgaaattt tactttcctg gcggatctta tttgaatatt cactgtcttt ctccatctcc
     5701 gtatcaatcg gaaaccccat aatgtacatc agtttaaaat tactccggcc aggcagatcc
     5761 acataatgtg gtaatgcaat tgtaatcgaa ttagcttcaa aatttggtct gtaactgctt
     5821 aatgtacttc cggaaaagag aaaagccgga acaccacctg aaccattcac taccattgta
     5881 tctgacataa aaattcctct ttaacacata aaaaaaacaa taagttaaaa aaatactgta
     5941 cataaaagca ctgtttttat gtacagtaat aaaattacgc cgctttattt tctctgtcaa
     6001 taatatgaaa tttcattttt gtgatctgaa tcactcttat aaaaatcagg aagggaagat
     6061 tcgcagcaga aaaacagcac cgggtaacat cagaaaaaaa cagaaaggag ataacgtgag
     6121 caaaacaaaa tctggtcgcc accgactgag caaaacagac aaacgcctgc tggctgcact
     6181 tgtcgttgcc ggatacgaag aacggacagc ccgtgacctc atccagaaac acgtttacac
     6241 actgacacag gccgacctgc gccatctggt cagtgaaatc agtaacggtg tgggacagtc
     6301 acaggcctac gatgcgattt accaggcgag acgcattcgt ctcgcccgta aatacctgag
     6361 cggaaaaaaa ccggaagggg tggaaccccg ggaagggcag gaacgggaag atttaccata
     6421 actcccgtta tcagtaccat cggctcaacg ctcgttgtcg gatctgaaaa attcgctcaa
     6481 aagatcatat ttccctggat attttccacc gtttcttatg tgagaaaagt cacataattc
     6541 tgtcagacga cgagaaaacg gatatcgatt attgtttaat atttttacat tattaaaaat
     6601 gaaattagat aatcagatac aaataatatg ttttcgttca tgcagagaga ttaagggtgt
     6661 ctaatgaaga aaagttctat tgtggcaacc attataacta ttctgtccgg gagtgctaat
     6721 gcagcatcat ctcagttaat accaaatata tcccctgaca gctttacagt tgcagcctcc
     6781 accgggatgc tgagtggaaa gtctcatgaa atgctttatg acgcagaaac aggaagaaag
     6841 atcagccagt tagactggaa gatcaaaaat gtcgctatcc tgaaaggtga tatatcctgg
     6901 gatccatact catttctgac cctgaatgcc agggggtgga cgtctctggc ttccgggtca
     6961 ggtaatatgg atgactacga ctggatgaat gaaaatcaat ctgagtggac agatcactca
     7021 tctcatcctg ctacaaatgt taatcatgcc aatgaatatg acctcaatgt gaaaggctgg
     7081 ttactccagg atgagaatta taaagcaggt ataacagcag gatatcagga aacacgtttc
     7141 agttggacag ctacaggtgg ttcatatagt tataataatg gagcttatac cggaaacttc
     7201 ccgaaaggag tgcgggtaat aggttataac cagcgctttt ctatgccata tattggactt
     7261 gcaggccagt atcgcattaa tgattttgag ttaaatgcat tatttaaatt cagcgactgg
     7321 gttcgggcac atgataatga tgagcactat atgagagatc ttactttccg tgagaagaca
     7381 tccggctcac gttattatgg taccgtaatt aacgctggat attatgtcac acctaatgcc
     7441 aaagtctttg cggaatttac atacagtaaa tatgatgagg gcaaaggagg tactcagatc
     7501 attgataaga atagtggaga ttctgtctct attggcggag atgctgccgg tatttccaat
     7561 aaaaattata ctgtgacggc gggtctgcaa tatcgcttct gaaaaataca gatcatatct
     7621 ctcttttcat cctcccctag cggggaggat gtctgtggaa aggaggttgg tgtttgacca
     7681 accttcagat gtgtgaaaaa tcaccttttt caccataatg acggggcgct cattctgttg
     7741 ttttgccttg acattctcca cgtctttcag ggcatggaga aggtcaaatt agacatggaa
     7801 cgctactctc cttcctgtag gaagctcaac atccaagctt aatttgcctc ccattgcttc
     7861 aacgtaacgc tttaacgtcg ccagctttaa atcatttccg cgctgctcca gctttgttac
     7921 tgctggctgg cttataccca tcgcctcagc aacttgtttt tgtgataact ggagttcttc
     7981 acgcatcatc tgcaagccga cctcaagaat catctcatct gccatttctt taattcgtgt
     8041 ctggctttca ggtgaacgac tggcaatcac ctcatctaat gttctcatta cttgctctcc
     8101 agtgtgttca gatgtgctgt aaattcatcc tcagctatac gcaccagttt ttcataaaac
     8161 cgcttatcat tacttttatc tcctgcacaa agaacgatag cccgacgaat cggatcgaac
     8221 gcataaaagg ctcttatcgg acggccagaa aactgaacgc gaagctcttt catatttttg
     8281 taccgagaac ctttcacggt atcggcatat ggcctgggta actcaggtcc gtaaacctgt
     8341 agctttttca aatcagccaa aaccttttcc tgaagagcgt cttcttgctc atttagccag
     8401 tcgtcaaatc gctggctaaa aagtaccatc cacatgctca accctataac ctgtagctta
     8461 ccccactaac aatataacct acgagttata ttttcaagaa aagctggcta tttaacataa
     8521 cggcaatttg tacgcaccac tgaaatgcgt tcagcgcgat cacggcaaca gacaggcaaa
     8581 aatagcaaca aacctcccga aaaaccgccg cgatcgcgcc tgataaattt taaccttatg
     8641 catatctatg cagccaggcg aatcacgaac gaattgcctg cctgatgtaa ctgaaacggg
     8701 tgttttttcc tgatttggtg ggcgtggaag acggaacatg aacgggaaaa cagaattcat
     8761 gccagatgag cgcgatctgg caattaaggc aaaacacagc aacaaagaca cgccagaatc
     8821 gcgcccggat atgttttaac gcgattttca gactcagaca aattcagcag aatgctactc
     8881 cattcaccgg gctgatggtg aatacatgcg tatccaggat gagtacattt ctggctctgc
     8941 cacagctctg tctgttggca gctttcgcct gtccggaaac ctgcttaaaa cgctcccgaa
     9001 aggcctctga accagaaagc aacaaaacac aggccattaa gtaaatcgcg ttaaaacacg
     9061 tctgatggat tgctgcaaaa aaaagtccct aatggagcag ggactgttaa acccagtgaa
     9121 tagcgtctaa attaaagtaa gaatacgacc aggtactctt cagaaaagag attaatccac
     9181 cgcacagaat aatcaacagt aaaaacaaac aaccctgatt ttttattttt ctttttttcg
     9241 ataaaaacaa aattaaagaa ataattaatc agaacattcc ttaacttcag ggcattgcct
     9301 gtgttccatt ttgtgattag tctgaaactt ccgaaggtgg ataacacccg gtattttttt
     9361 gctcacataa agcccctcct tcaggcagag gggctttttc tttgccacca cataaaaaag
     9421 gccctcacag gaggtgttct gtgagggcgt atgataagga ctgaatcgat ggttaatatg
     9481 tctagtcctg acttttgcat ctccgaatat aaaaccctgt ttaacggcat gcaaaaccaa
     9541 aaaataaaaa tgtgacatcg caatgccaga taatattgac gcatgaggga atgcgtaccc
     9601 cgacccctg
//