^PLATFORM = Murine 15K long oligo array version 2.0
!Platform_title = Murine 15K long oligo array version 2.0
!Platform_technology = spotted oligonucleotide
!Platform_distribution = non-commercial
!Platform_organism = Mus musculus
!Platform_manufacturer = Un. London microarray facility
!Platform_manufacture_protocol =    1.  Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
!Platform_manufacture_protocol =    2. Resuspend oligos in water to 20 uM and rearray 5 L into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
!Platform_manufacture_protocol =    3. Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood).
!Platform_manufacture_protocol =    4. Before printing, add 5 L of 1X Printing Buffer to each well. This can be done the night before a print run is started.
!Platform_manufacture_protocol =    5. Seal plates with Corning seals.
!Platform_manufacture_protocol =    6. Incubate at 37C for 30 minutes to aid resuspension of DNA.
!Platform_manufacture_protocol =    7. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
!Platform_manufacture_protocol =    8. Centrifuge plates at 2000 rpm for 3 minutes.
!Platform_manufacture_protocol =    9. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30 minutes before next cassette is needed to begin centrifugation.
!Platform_manufacture_protocol =   10. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
!Platform_manufacture_protocol =   11. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.	
!Platform_manufacture_protocol =   12. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer. The amount of water should be decreased by 0.25 L per print run, as this is the amount drawn up by the pin capillary during each dip.	
!Platform_support = glass
!Platform_coating = polysine	
!Platform_web_link = http://www.microarray.protocols.html
!Platform_contributor = Jane,Doe
!Platform_contributor = John,A,Smith
!Platform_contributor = Hans,van Elton
!Platform_contributor = John,Smithers Jr
!Platform_contributor = Jie,D,Chen
#ID = 
#GB_ACC = GenBank accession number of sequence used to design oligonucleotide probe   LINK_PRE:"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&term=" 
#Gene_Desc = Gene description
#Gene_Sym = Gene symbols
#SEQUENCE = Probe sequence information
#SPOT_ID = alternative identifier
!platform_table_begin
ID	GB_ACC	Gene_Desc	Gene_Sym	SPOT_ID	SEQUENCE
1	U02079	nuclear factor of activated T-cells, cytoplasmic 2	Nfatc2		ACCTGGATGACGCAGCCACTTCAGAAAGCTGGGTTGGGACAGAAAGGTATATAGAGAGAAAATTTTGGAA
2	NM_008154	G-protein coupled receptor 3	Gpr3		CTGTACAATGCTCTCACTTACTACTCAGAGACAACGGTAACTCGGACTTATGTGATGCTGGCCTTGGTGT
3	AK015719	tropomodulin 2	Tmod2		CACCAGGCTCAGTGCCTAGTATCGGCTTCACCTAGTGTGGTTACTCAGGGCACGCAGAGCTACAGAACAC
4	AK003367	mitochondrial ribosomal protein L15	Mrpl15		CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAAT
5	BC003333	RIKEN cDNA 0610033I05 gene	0610033I05Rik		AGAACTGGGTGGCAGATATCCTAGAGTTTTGACCAACGTTCACAGCACACATATTGATCTTATAGGACCT
6	NM_008462	killer cell lectin-like receptor, subfamily A, member 2	Klra2		TGAATTGAAGTTCCTTAAATCCCAACTTCAAAGAAACACATACTGGATTTCACTGACACATCATAAAAGC
7	NM_008029	FMS-like tyrosine kinase 4	Flt4		GAGGTGCTGTGGGATGACCGCCGGGGCATGCGGGTGCCCACTCAACTGTTGCGCGATGCCCTGTACCTGC
8	NM_054088	adiponutrin	Adpn		GTCTGAGTTCCATTCCAAAGACGAAGTCGTGGATGCCCTGGTGTGTTCCTGCTTCATTCCCCTCTTCTCT
9	NM_009750	nerve growth factor receptor (TNFRSF16) associated protein 1	Ngfrap1		TACAGCTGAGAAATTGTCTACGCATCCTTATGGGGGAGCTGTCTAACCACCACGATCACCATGATGAATT
10	AB045323	DNA segment, Chr 8, ERATO Doi 594, expressed	D8Ertd594e		GATTCAGACTCGGGAGGAGCATCCCAACCTCTCCTTGAGGATAAAGGCCTGAGCGATTGCCCTGGGGAGC
11	AK005789	dynein, cytoplasmic, light chain 2B	Dncl2b		TGCAGAAGGCATTCCAATCCGAACAACCCTGGACAACTCCACAACGGTTCAGTATGCGGGTCTTCTCCAC
12	NM_010517	insulin-like growth factor binding protein 4	Igfbp4		GGAGAAGCTGGCGCGCTGCCGCCCCCCCGTGGGTTGCGAGGAGTTGGTGCGGGAGCCAGGCTGCGGTTGT
13	AK010722	RIKEN cDNA 2410075D05 gene	2410075D05Rik		GGAGCATCTGGAGTTCCGCTTACCGGAAATAAAGTCTTTACTATCGGTGATTGGAGGGCAGTTCACTAAC
14	AK003755	DNA segment, Chr 4, ERATO Doi 421, expressed	D4Ertd421e		AGCAAAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCTTGCGGTTATTGGCCAGTGACTTGC
15	BC003241	cleavage stimulation factor, 3' pre-RNA, subunit 3	Cstf3		AAATTAGAAGAAAATCCATATGACCTTGATGCTTGGAGCATTCTCATTCGAGAGGCACAGAATCAACCTA
16	AK004937	RIKEN cDNA 1300007O09 gene	1300007O09Rik		CAGACACAAACCCTAGGTTGTATTGTAGACCGGAGTTTAAGCAGGCACTACCTGTCTGTCTTTTCTTCAT
17	AK004524	unnamed protein product; hypothetical SOCS domain			CGGAGCCCTGCGCGCCCAGAGCCCCCTCCCACCCGCTTCCACCAAGTGCATGGAGCCAACATCCGCATGG
18	NM_025999	RIKEN cDNA 2610110L04 gene	2610110L04Rik		TGCATTGATAAATGGAGTGATCGACACAGGAACTGCCCCATTTGTCGCCTACAGATGACTGGAGCAAATG
19				-- CONTROL	
20	NM_023120	guanine nucleotide binding protein (G protein), beta polypeptide 1-like	Gnb1l		ACCGCCTGGTCCCAGATTTGTCCTCCGAGGCACACAGTCGGCTGTGAACACGCTCCATTTCTGCCCACCA
!platform_table_end