BLASTP 2.0.8 [Jan-05-1999]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= YDL043C PRP11, Chr IV from 375676-376476, reverse complement
         (266 letters)

Database: nr
           361,503 sequences; 110,384,460 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gi|2498800|sp|Q07350|PR11_YEAST PRE-MRNA SPLICING FACTOR PRP11 ...   467  e-131
gi|1279673|emb|CAA96446| (Z71781) RNA11 [Saccharomyces cerevisiae]   460  e-129
gi|3289979 (AC005263) SP62_HUMAN; SAP 62; SF3A66 [Homo sapiens]       68  5e-11
gi|2498942|sp|Q62203|SP62_MOUSE SPLICEOSOME ASSOCIATED PROTEIN ...    68  5e-11
gi|2498941|sp|Q15428|SP62_HUMAN SPLICEOSOME ASSOCIATED PROTEIN ...    68  7e-11
gi|3875770|emb|CAA92593| (Z68297) Similarity to Human splicosom...    65  4e-10
gi|3298548 (AC004681) putative spliceosomal protein [Arabidopsi...    57  2e-07

>gi|2498800|sp|Q07350|PR11_YEAST PRE-MRNA SPLICING FACTOR PRP11
           >gi|2133073|pir||S67576 PRP11 protein - yeast
           (Saccharomyces cerevisiae) >gi|1431030|emb|CAA98602|
           (Z74091) ORF YDL043c [Saccharomyces cerevisiae]
           >gi|226088|prf||1410273A RNA11 gene [Saccharomyces
           cerevisiae]
           Length = 266
           
 Score =  467 bits (1188), Expect = e-131
 Identities = 233/252 (92%), Positives = 233/252 (92%)

Query: 1   MNYLEXXXXXXXXXXIASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYK 60
           MNYLE          IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYK
Sbjct: 1   MNYLEGVGSKKGGGGIASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYK 60

Query: 61  NHSGKLVCKLCNTMHMSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQ 120
           NHSGKLVCKLCNTMHMSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQ
Sbjct: 61  NHSGKLVCKLCNTMHMSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQ 120

Query: 121 KIIEAKQSLKNNGTIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLI 180
           KIIEAKQSLKNNGTIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLI
Sbjct: 121 KIIEAKQSLKNNGTIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLI 180

Query: 181 RIVSGLELSDTKQKGKKFLVIAYEPFENIAIELPPNEILFSEXXXXXXXXXGVDELNKKC 240
           RIVSGLELSDTKQKGKKFLVIAYEPFENIAIELPPNEILFSE         GVDELNKKC
Sbjct: 181 RIVSGLELSDTKQKGKKFLVIAYEPFENIAIELPPNEILFSENNDMDNNNDGVDELNKKC 240

Query: 241 TFWDAISKLYYV 252
           TFWDAISKLYYV
Sbjct: 241 TFWDAISKLYYV 252

>gi|1279673|emb|CAA96446| (Z71781) RNA11 [Saccharomyces cerevisiae]
           Length = 263
           
 Score =  460 bits (1170), Expect = e-129
 Identities = 228/237 (96%), Positives = 228/237 (96%)

Query: 16  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 75
           IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH
Sbjct: 13  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 72

Query: 76  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI 135
           MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI
Sbjct: 73  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI 132

Query: 136 PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELSDTKQKG 195
           PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELSDTKQKG
Sbjct: 133 PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELSDTKQKG 192

Query: 196 KKFLVIAYEPFENIAIELPPNEILFSEXXXXXXXXXGVDELNKKCTFWDAISKLYYV 252
           KKFLVIAYEPFENIAIELPPNEILFSE         GVDELNKKCTFWDAISKLYYV
Sbjct: 193 KKFLVIAYEPFENIAIELPPNEILFSENNDMDNNNDGVDELNKKCTFWDAISKLYYV 249

>gi|3289979 (AC005263) SP62_HUMAN; SAP 62; SF3A66 [Homo sapiens]
           Length = 464
           
 Score = 68.3 bits (164), Expect = 5e-11
 Identities = 57/204 (27%), Positives = 90/204 (43%), Gaps = 32/204 (15%)

Query: 16  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 75
           +AS S+ N  RR+ +  L  +      T    KD      PY  KNH G   CKLC T+H
Sbjct: 16  VASSSESNRDRRERLRQLALE------TIDINKD------PYFMKNHLGSYECKLCLTLH 63

Query: 76  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI 135
            +  S   H  GKKH  N+ RR     K +  +          ++  +E K+ +K     
Sbjct: 64  NNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPA-------PEKVKVEVKKFVKIGR-- 114

Query: 136 PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELS-DTKQK 194
           P  K+   ++ + G   L  Q++Y  E+ E          + P  R +S  E   +   +
Sbjct: 115 PGYKVTKQRDSEMGQQSLLFQIDY-PEIAEG---------IMPRHRFMSAYEQRIEPPDR 164

Query: 195 GKKFLVIAYEPFENIAIELPPNEI 218
             ++L++A EP+E IA ++P  EI
Sbjct: 165 RWQYLLMAAEPYETIAFKVPSREI 188

>gi|2498942|sp|Q62203|SP62_MOUSE SPLICEOSOME ASSOCIATED PROTEIN 62
           (SAP 62) (SF3A66) >gi|633251|emb|CAA58703| (X83733)
           spliceosome SAP62 [Mus musculus]
           Length = 485
           
 Score = 68.3 bits (164), Expect = 5e-11
 Identities = 57/204 (27%), Positives = 90/204 (43%), Gaps = 32/204 (15%)

Query: 16  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 75
           +AS S+ N  RR+ +  L  +      T    KD      PY  KNH G   CKLC T+H
Sbjct: 16  VASSSESNRDRRERLRQLALE------TIDINKD------PYFMKNHLGSYECKLCLTLH 63

Query: 76  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI 135
            +  S   H  GKKH  N+ RR     K +  +          ++  +E K+ +K     
Sbjct: 64  NNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPA-------PEKVKVEVKKFVKIGR-- 114

Query: 136 PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELS-DTKQK 194
           P  K+   ++ + G   L  Q++Y  E+ E          + P  R +S  E   +   +
Sbjct: 115 PGYKVTKQRDTEMGQQSLLFQIDY-PEIAEG---------IMPRHRFMSAYEQRIEPPDR 164

Query: 195 GKKFLVIAYEPFENIAIELPPNEI 218
             ++L++A EP+E IA ++P  EI
Sbjct: 165 RWQYLLMAAEPYETIAFKVPSREI 188

>gi|2498941|sp|Q15428|SP62_HUMAN SPLICEOSOME ASSOCIATED PROTEIN 62
           (SAP 62) (SF3A66) >gi|627605|pir||A47655
           spliceosome-associated protein SAP 62 - human >gi|409219
           (L21990) spiceosomal protein [Homo sapiens]
           Length = 464
           
 Score = 67.9 bits (163), Expect = 7e-11
 Identities = 57/204 (27%), Positives = 90/204 (43%), Gaps = 32/204 (15%)

Query: 16  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 75
           +AS S+ N  RR+ +  L  +      T    KD      PY  KNH G   CKLC T+H
Sbjct: 16  VASSSESNRDRREPLRQLALE------TIDINKD------PYFMKNHLGSYECKLCLTLH 63

Query: 76  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDFRQQQKIIEAKQSLKNNGTI 135
            +  S   H  GKKH  N+ RR     K +  +          ++  +E K+ +K     
Sbjct: 64  NNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPA-------PEKVKVEVKKFVKIGR-- 114

Query: 136 PVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELS-DTKQK 194
           P  K+   ++ + G   L  Q++Y  E+ E          + P  R +S  E   +   +
Sbjct: 115 PGYKVTKQRDSEMGQQSLLFQIDY-PEIAEG---------IMPRHRFMSAYEQRIEPPDR 164

Query: 195 GKKFLVIAYEPFENIAIELPPNEI 218
             ++L++A EP+E IA ++P  EI
Sbjct: 165 RWQYLLMAAEPYETIAFKVPSREI 188

>gi|3875770|emb|CAA92593| (Z68297) Similarity to Human
           splicosome-associated protein SAP62 (PIR Acc. No.
           A47655); cDNA EST EMBL:C08866 comes from this gene; cDNA
           EST yk247a3.3 comes from this gene; cDNA EST yk247a3.5
           comes from this gene; cDNA EST yk371...
           Length = 610
           
 Score = 65.2 bits (156), Expect = 4e-10
 Identities = 61/240 (25%), Positives = 99/240 (40%), Gaps = 51/240 (21%)

Query: 16  IASESQFNLQRRKEVESLLSKGENVPYTFQDEKDDQVRSNPYIYKNHSGKLVCKLCNTMH 75
           +AS +   + RR+ +  L  +      T   +KD      PY  +NH G   CKLC T+H
Sbjct: 16  VASAADAGVDRRERLRQLALE------TIDLQKD------PYFMRNHIGTYECKLCLTLH 63

Query: 76  MSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTHDF--RQQQKIIEAKQSLKNNG 133
            +  S   H  GKKH  N+ RR           + Q+   F    Q+  +E K+ +K   
Sbjct: 64  NNEGSYLAHTQGKKHQANLARR---------AAKEQSEQPFLPAPQKAAVETKKFVKIGR 114

Query: 134 TIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDKAKVPPLIRIVSGLELS-DTK 192
             P  K+   ++P  G   L  Q++Y  E+ +          + P  R +S  E      
Sbjct: 115 --PGYKVTKERDPGAGQQALLFQIDY-PEIADG---------IAPRHRFMSAYEQKIQPP 162

Query: 193 QKGKKFLVIAYEPFENIAIELPPNEILFSEXXXXXXXXXGVDELNKKCTFWDAISKLYYV 252
            K  ++L+ A EP+E I  ++P  E               VD+  K  T W+  +K +++
Sbjct: 163 DKRWQYLLFAAEPYETIGFKIPSRE---------------VDKSEKFWTMWNKDTKQFFL 207

>gi|3298548 (AC004681) putative spliceosomal protein [Arabidopsis
           thaliana]
           Length = 275
           
 Score = 56.6 bits (134), Expect = 2e-07
 Identities = 44/172 (25%), Positives = 72/172 (41%), Gaps = 34/172 (19%)

Query: 55  NPYIYKNHSGKLVCKLCNTMHMSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQTTH 114
           +PY  +NH G   CKLC T+H +  +   H  GK+H  N+ +R                 
Sbjct: 41  DPYFMRNHLGSYECKLCLTLHNNEGNYLAHTQGKRHQTNLAKRAA--------------- 85

Query: 115 DFRQQQKIIEAKQSLKNNGTIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSV------ 168
             R+ +      Q LK N       ++  +  K G  G  +   Y  E+++ S+      
Sbjct: 86  --REAKDAPTKPQPLKRN-------VSVRRTVKIGRPGYRVTKQYDPELQQRSLLFQIEY 136

Query: 169 -DSDDKAKVPPLIRIVSGLELS-DTKQKGKKFLVIAYEPFENIAIELPPNEI 218
            + +D  K  P  R +S  E       K  ++L+ A EP+E IA ++P  E+
Sbjct: 137 PEIEDNIK--PRHRFMSSYEQKVQPYDKSYQYLLFAAEPYEIIAFKVPSTEV 186

  Database: nr
    Posted date:  Mar 13, 1999  7:20 PM
  Number of letters in database: 110,384,460
  Number of sequences in database:  361,503
  
Lambda     K      H
   0.313    0.132    0.374 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50178902
Number of Sequences: 361503
Number of extensions: 1948558
Number of successful extensions: 3688
Number of sequences better than 1.0e-03: 7
Number of HSP's better than  0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3682
Number of HSP's gapped (non-prelim): 7
length of query: 266
length of database: 110384460
effective HSP length: 57
effective length of query: 209
effective length of database: 89778789
effective search space: 18763766901
effective search space used: 18763766901
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 42 (21.9 bits)
S2: 102 (44.1 bits)