# BEGIN SURVEY

++++++++++

Answers to questions within Sections 1 through 4 of the survey provide
general information about your hatchery, the fish cultured, its water
use, and the water body your hatchery effluent enters. Sections 1
through 4 are vitally important because they serve as the reference
point for all of the treatment regimen information requested within
Section 5 of the survey.

In Section 5, we ask you to provide treatment regimen information to
describe treatment regimens you currently use or would anticipate using
to prevent or control pathogens in the next five years. ***[We
understand that the answers provided in Section 5 are based on the
assumption that the chemicals are, or will be, legally available for use
either with an approved label or via INAD.]{.underline}***

Remember to keep all answers to the right of the colon. Answers are not
case-sensitive, and answers are not required for each question (i.e.,
blank lines are acceptable).

All main headings of sections are in bold Italics and section
subheadings are in Italics. All header and administrative portions of
the survey are separated from data entry lines by a series of asterisks
(\*). Survey questions are in bold (**i.e., the text to the left of the
colon**), if a suggested response example or unit of measure is
included, it is presented as an **[underlined bold response suggestion
or unit of measure (e.g., million gpd)]{.underline}**.

# [Please be sure to periodically save your file.]{.underline}

# Section 1 - Hatchery Information

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# Hatchery Name: UPPER MIDWEST ENVIRONMENTAL SCIENCES CENTER

# Contact Person: LYNN LEE

# Address: 2630 FANTA REED ROAD

# City: LA CROSSE

# State: WI

# Zip Code: 54603

# Phone number: 608-781-6249

# Fax number: 608-783-6066

# E-mail address:

# Section 2 - Species Cultured

Please enter the name and life stage of the species most commonly
cultured at your facility, even those you typically would not treat.
Species held at your facility for only a brief period (i.e., less than a
week) before transfer or those brought in for forage (other than fish
routinely cultured on site for forage) do not need to be included.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# Species 1 (name): rainbow trout

# Species 1 (life stage cultured; [E, F, or B]{.underline}): b

# Species 2 (name): lake trout

# Species 2 (life stage cultured; [E, F, or B]{.underline}): f

# Species 3 (name): Atlantic salmon

# Species 3 (life stage cultured; [E, F, or B]{.underline}): b

# Species 4 (name): black bullhead

# Species 4 (life stage cultured; [E, F, or B]{.underline}): f

# Species 5 (name): black crappie

# Species 5 (life stage cultured; [E, F, or B]{.underline}): b

# Species 6 (name): bluegill

# Species 6 (life stage cultured; [E, F, or B]{.underline}): f

# Species 7 (name): northern pike

# Species 7 (life stage cultured; [E, F, or B]{.underline}): b

# Species 8 (name): brown trout

# Species 8 (life stage cultured; [E, F, or B]{.underline}): b

# Species 9 (name): brook trout

# Species 9 (life stage cultured; [E, F, or B]{.underline}): b

# Species 10 (name): muskellunge

# Species 10 (life stage cultured; [E, F, or B]{.underline}): b

# Species 11 (name): walleye

# Species 11 (life stage cultured; [E, F, or B]{.underline}): b

# Species 12 (name): lake sturgeon

# Species 12 (life stage cultured; [E, F, or B]{.underline}): b

# Species 13 (name): channel catfish

# Species 13 (life stage cultured; [E, F, or B]{.underline}): b

# Species 14 (name): fathead minnow

# **Species 14 (life stage cultured; [E, F, or B]{.underline}): **b

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# Section 3 - Hatchery Water Source and Use

Describe the physical and chemical characteristics of your hatchery
water, including how the water is treated before it leaves the hatchery
and what type of water body it enters after leaving the hatchery. Also,
please provide the amount of water your hatchery uses throughout the
year.

# Total Hatchery Water Use

Please estimate average hatchery water use.

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# Average Total Hatchery Daily Water Flow? ([million gpd]{.underline}): 0.75

# Lowest probable flow (million gpd): 0.5

In general, how would you describe your hatchery water? (**[X only
one]{.underline}**)

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# Freshwater?: X

# Brackish?:

# Water Chemistry Characteristics

# Temperature

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# Celcius or Farenheit? ([Enter C or F]{.underline}): F

# Temperature Average: 54

# Temperature Minimum: 46

# Temperature Maximum: 80

pH

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**pH Average: 8.10**

**pH Minimum: 8.08**

**pH Maximum: 8.19**

# Hardness (**[mg/L as CaCO]{.underline}~[3]{.underline}~**)

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# Hardness Average: 150

# Hardness Minimum: 100

# Hardness Maximum: 200

# Alkalinity (**[mg/L as CaCO]{.underline}~[3]{.underline}~**)

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# Alkalinity Average: 100

# Alkalinity Minimum: 80

# Alkalinity Maximum: 160

# Specific Conductivity (**[F]{.underline}[mhos/cm]{.underline}**)

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# Specific Conductivity Average: 280

# Specific Conductivity Minimum: 212

# Specific Conductivity Maximum: 360

Salinity (ppt)

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# Salinity Average:

# Salinity Minimum:

# Salinity Maximum:

# Enter in the other water chemistry parameters not listed in the above

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# Other Chemistry Type:

# Other Chemistry Type Average:

# Other Chemistry Type Minimum:

# Other Chemistry Type Maximum:

# Effluent Water Treatment and Discharge

The following units of measure are used within this section of the
survey;

**[acre-foot ]{.underline}**- the volume of water that would cover one
acre one foot deep; also equals 325850 gallons**[ ]{.underline}**

**[cfs]{.underline}** - cubic feet per second

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# Does hatchery effluent pass through a settling pond before discharge?([Y/N]{.underline}): Y

# If yes, what is the settling pond volume? ([acre-feet]{.underline}): 22.2

# Hatchery has a National Pollution Discharge Elimination System (NPDES) permit? ([Y/N]{.underline}): Y

# Hatchery has a State Pollution Discharge Elimination System (SPDES) permit? ([Y/N]{.underline}):

What type of water body does your hatchery effluent enter? (**[X only
one]{.underline}**)

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# Lake/Pond:

# River/Stream:

# Backwater of a River/Stream: X

In general, how would you describe the water body you discharge into?
(**[X only one]{.underline}**)

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# Freshwater?: X

# Brackish?:

# Estuary?:

If your effluent enters a Lake/Pond, estimate the following.

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# If Lake/Pond selected, what is the estimated average volume? ([acre-feet]{.underline})?:

# Does the Lake/Pond discharge to a river or stream?([Y/N]{.underline}):

# If yes, what is the estimated flow of the river/stream ([cfs]{.underline}):

# Is the Lake/Pond discharge the stream's only water source? ([Y/N]{.underline}):

If your effluent enters a River/Stream, answer the following.

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# If River/Stream selected, what is the estimated average flow? ([cfs]{.underline}):

# The lowest flow occurs during what season? ([NC if no change]{.underline}):

# What is the estimated average flow during the low flow season? ([cfs]{.underline}):

If your effluent enters a River/Stream Backwater, answer the following.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# What is the Backwater volume in a typical year ([acre-feet]{.underline})?: 100

# What is the flow of the river/stream the backwater enters? ([cfs]{.underline}): 1800

# The lowest flow occurs during what season? ([NC if no change]{.underline}): WINTER

# What is the estimated average flow during the low flow season? ([cfs]{.underline}): 316

## Section 4 - Hatchery Culture Units

Please describe the number and types of fish culture units (egg
incubators, tanks, raceways, and ponds) your hatchery uses to incubate
eggs or culture fish. We understand that, unlike egg incubators, tanks,
raceways, and ponds come in a plethora of shapes and sizes. In the
spaces provided please provide information describing each of your three
most representative tanks, raceways, and ponds, particularly those in
which you would anticipate treating fish. For lack of a better label,
the fish culture units are referred to as Tank size 1, Tank size 2, Tank
size 3; Raceway size 1, Raceway size 2, Raceway size 3; Pond size 1,
Pond size 2, and Pond size 3. Survey questions seeking to describe your
hatchery treatment regimens will request the numbers of a given tank,
raceway, or pond treated of a given size. Please refer back to this
section when completing the treatment regimen descriptions.

This information will allow us to estimate \"worst-case\" treatment
scenarios in a typical hatchery.

# Egg Jars -- Size 1

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# Number of egg banks - Size 1: 1

# Average number of jars/bank - Size 1: 10

# Minimum number of jars/bank - Size 1: 1

# Maximum number of jars/bank - Size 1: 10

# Average flow rate/jar - Size 1 ([gpm]{.underline}): 1.5

# Minimum flow rate/jar - Size 1 ([gpm]{.underline}): 1

# Maximum flow rate/jar - Size 1 ([gpm]{.underline}): 2.5

# Egg Jars -- Size 2

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# Number of egg banks - Size 2:

# Average number of jars/bank - Size 2:

# Minimum number of jars/bank - Size 2:

# Maximum number of jars/bank - Size 2:

# Average flow rate/jar - Size 2 ([gpm]{.underline}):

# Minimum flow rate/jar - Size 2 ([gpm]{.underline}):

# Maximum flow rate/jar - Size 2 ([gpm]{.underline}):

# Heath Trays

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# Number of stacks: 2

# Average number of trays/stack: 8

# Minimum number of trays/stack: 1

# Maximum number of trays/stack: 8

# Average flow rate/stack ([gpm]{.underline}): 5

# Minimum flow rate/stack ([gpm]{.underline}): 2

# Maximum flow rate/stack ([gpm]{.underline}): 7

*Clark-Williams (trough incubators)*

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# Number of raceways or troughs:

# Average number of compartments:

# Minimum number of compartments:

# Maximum number of compartments:

# Average flow rate / raceway or trough ([gpm]{.underline}):

# Minimum flow rate / raceway or trough ([gpm]{.underline}):

# Maximum flow rate / raceway or trough ([gpm]{.underline}):

# Fish Culture Units -- Tanks and Raceways

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# What is the volume of Tank size 1 ([gallons]{.underline}): 385

# Number of tanks at Tank size 1: 39

# Average flow rate to Tank size 1 ([gpm]{.underline}): 6

# Minimum flow rate to Tank size 1 ([gpm]{.underline}): 5

# Maximum flow rate to Tank size 1 ([gpm]{.underline}): 20

# What is the volume of Tank size 2 ([gallons]{.underline}): 187

# Number of tanks at Tank size 2: 2

# Average flow rate to Tank size 2 ([gpm]{.underline}): 5

# Minimum flow rate to Tank size 2 ([gpm]{.underline}): 3

# Maximum flow rate to Tank size 2 ([gpm]{.underline}): 20

# What is the volume of Tank size 3 ([gallons]{.underline}):

# Number of tanks at Tank size 3:

# Average flow rate to Tank size 3 ([gpm]{.underline}):

# Minimum flow rate to Tank size 3 ([gpm]{.underline}):

# Maximum flow rate to Tank size 3 ([gpm]{.underline}):

# What is the volume of Raceway size 1 ([gallons]{.underline}): 2047

# Number of raceways at Raceway size 1: 10

# Average flow rate to Raceway size 1 ([gpm]{.underline}): 34

# Minimum flow rate to Raceway size 1 ([gpm]{.underline}): 34

# Maximum flow rate to Raceway size 1 ([gpm]{.underline}): 150

# What is the volume of Raceway size 2 ([gallons]{.underline}): 3044

# Number of raceways at Raceway size 2: 2

# Average flow rate to Raceway size 2 ([gpm]{.underline}): 51

# Minimum flow rate to Raceway size 2 ([gpm]{.underline}): 51

# Maximum flow rate to Raceway size 2 ([gpm]{.underline}): 150

# What is the volume of Raceway size 3 ([gallons]{.underline}):

# Number of raceways at Raceway size 3:

# Average flow rate to Raceway size 3 ([gpm]{.underline}):

# Minimum flow rate to Raceway size 3 ([gpm]{.underline}):

# Maximum flow rate to Raceway size 3 ([gpm]{.underline}):

# Fish Culture Units -- Ponds

**[acre-foot ]{.underline}**- the volume of water that would cover one
acre one foot deep; also equals 325850 gallons

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# Is water flow to Pond size 1, 2, or 3 to make-up evaporation/leakage? ([Y/N]{.underline}): Y

# Is Pond out-flow intermittent, e.g., only during pond drainage/harvest? ([Y/N]{.underline}): Y

# What is the volume of Pond size 1 ([acre-feet]{.underline}): 0.4

# Number of ponds at Pond size 1: 18

# Average flow rate to Pond size 1 ([gpm]{.underline}): 200

# Minimum flow rate to Pond size 1 ([gpm]{.underline}): 50

# Maximum flow rate to Pond size 1 ([gpm]{.underline}): 600

# What is the volume of Pond size 2 ([acre-feet]{.underline}): 2.5

# Number of ponds at Pond size 2: 1

# Average flow rate to Pond size 2 ([gpm]{.underline}): 200

# Minimum flow rate to Pond size 2 ([gpm]{.underline}): 50

# Maximum flow rate to Pond size 2 ([gpm]{.underline}): 600

# What is the volume of Pond size 3 ([acre-feet]{.underline}): 5

# Number of ponds at Pond size 3: 2

# Average flow rate to Pond size 3 ([gpm]{.underline}): 200

# Minimum flow rate to Pond size 3 ([gpm]{.underline}): 50

# Maximum flow rate to Pond size 3 ([gpm]{.underline}): 600

# Section 5- Chemical Treatments

From the list of chemicals provided below, please describe your typical
treatment and anesthetic practices. ***[Also include those treatments
you would use provided you have legal access to the drug through an
approved label or an INAD.]{.underline}*** If you do not have experience
with these drugs but anticipate needing to use them, supply your best
guess at the dose or concentration based on prior knowledge with similar
drugs.

The following chemicals will likely be approved for use on both fish and
fish eggs. Please place an **[E (eggs), F (fish), or B
(both)]{.underline}** to indicate the life stages you will treat or hope
to treat using these chemicals in the next 5 years at your hatchery.
***[We understand that the answers provided to this question and in
treatment regimen descriptions are based on the assumption that the
chemicals are, or will be, legally available for use (either with an
approved label or via an INAD).]{.underline}***

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

**hydrogen peroxide (fish -- 50 to 250FL/L; eggs -- 500 to 1000 FL/L)?
([E, F, or B]{.underline}): B**

**potassium permanganate (0.25 to 8 mg/L)? ([E, F, or B]{.underline}):
**

The following chemicals will likely be approved for use only on fish.
Please place a **[Y/N]{.underline}** to indicate whether or not you will
use or hope to use these chemicals in the next 5 years to treat fish at
your hatchery. ***[We understand that the answers provided to this
question and in treatment regimen descriptions are based on the
assumption that the chemicals are, or will be, legally available for use
(either with an approved label or via an INAD).]{.underline}***

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# Aqui-S (should be from 25 to 50 mg/L) ([Y/N]{.underline}): Y

# Chloramine-T (allowable limit is 10 to 20 mg/L for four treatments) ([Y/N]{.underline}): Y

# Florfenicol (allowable limit is 10 mg/kg for 10 d) ([Y/N]{.underline}): Y

# Oxytetracycline (static immersion bath; 10 to 50 mg/L) ([Y/N]{.underline}): Y

# Treatment Regimens

The treatment regimen information you will provide at this point in the
survey is one of the most important portions of the survey. The
treatment regimens are separated into an Oral Drug Treatment Regimen
(OR), eight Water-borne Treatment Regimens (TR), and two Anesthetic
Regimens (AR). Florfenicol is the only oral drug that we currently
anticipate writing a portion of the Environmental Assessment.

Please describe your treatments as thoroughly as possible. Although the
survey attempts to consolidate as many different treatment scenarios as
possible into one treatment regimen, some cases require submission of
multiple treatment regimens for one chemical. For instance, hydrogen
peroxide is administered at much greater concentrations and for a
greater number of exposures to control fungus on eggs than when used to
control fungus, bacteria, or parasites on fish. Your responses will form
the basis of our Environmental Assessment that tells the U.S. Food and
Drug Administration how chemicals are used, how often they are
administered, and potentially how much may enter the environment.

Please see the examples for water borne and oral drug treatment regimens
in the completed example surveys attached as "example.doc" (MS Word97)
or "example.wpd" (WordPerfect 6/7/8).

If you wish to describe additional treatment regimens, copy the
information from one of the treatment regimens and paste it at the end
of the document. Please state that additional treatment regimens were
added to the survey in the body of your e-mail message when you return
the survey to UMESC (applies only to electronically submitted surveys).

\########### **Please Enter Oral Drug Treatment Regimens on the
following page** \###########

*Oral Drug Treatment Regimen (OR) 1 - ****Florfenicol at 10 mg/kg for 10
days***

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# OR 1 - BGD: X

# OR 1 - Columnaris / BCWD: X

# OR 1 - furunculosis / Aeromonas hydrophilia: X

# OR 1 - BKD / ERM: X

# OR 1 - other: X

# If checked OR 1 - other, enter [disease name]{.underline}: ENTERIC SEPTICEMIA

# What types of fish are treated (**[X all that apply]{.underline}**)?

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# OR 1 - Coldwater: X

# OR 1 - Coolwater: X

# OR 1 - Warmwater: X

Please give the maximum number of culture units treated on a given day
and the average fish mass (**[kg]{.underline}**) treated in a given
culture unit. (Note - you entered culture unit size information
beginning on page 10 {depending on printer})

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# OR 1 - tank size 1: 3

# **OR 1 - average treated biomass in tank size 1 **(**[kg]{.underline}**)**: 11**

# OR 1 - tank size 2: 2

# **OR 1 - average treated biomass in tank size 2 **(**[kg]{.underline}**)**: 11**

# OR 1 - tank size 3:

# **OR 1 - average treated biomass in tank size 3 **(**[kg]{.underline}**)**: **

# OR 1 - raceway size 1: 1

# **OR 1 - average treated biomass in raceway size 1 **(**[kg]{.underline}**)**: 250**

# OR 1 - raceway size 2: 1

# **OR 1 - average treated biomass in raceway size 2 **(**[kg]{.underline}**)**: 250**

# OR 1 - raceway size 3:

# **OR 1 - average treated biomass in raceway size 3 **(**[kg]{.underline}**)**: **

# OR 1 - pond size 1:

# **OR 1 - average treated biomass in pond size 1 **(**[kg]{.underline}**)**: **

# OR 1 - pond size 2:

# **OR 1 - average treated biomass in pond size 2 **(**[kg]{.underline}**)**: **

# OR 1 - pond size 3:

# **OR 1 - average treated biomass in pond size 3 **(**[kg]{.underline}**)**: **

# How often would you typically administer this treatment regimen?

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# OR 1 - times per year (enter number): 6

When do you typically treat? (**[X all that apply]{.underline}**)

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# OR 1 - spring: X

# OR 1 - summer: X

# OR 1 - fall: X

# OR 1 - winter: X

\###### **Please Enter Water-borne Chemical Treatment Regimens on the
following page** \#######

*Water-borne Chemical Treatment Regimen (TR) 1*

Please select the chemical described in this treatment regimen (only one
chemical per treatment regimen description) and identify the life stage
treated by placing an **[E (eggs) or F (fish)]{.underline}** to the
right of the colon for the appropriate chemical.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 1 - hydrogen peroxide: F

# TR 1 - chloramine-T:

# TR 1 - oxytetracycline:

# TR 1 - potassium permanganate:

# What is the dose administered?

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# TR 1 - water minimum ([mg/L]{.underline}):

# TR 1 - water maximum ([mg/L]{.underline}):

# TR 1 - water minimum ([uL/L]{.underline}): 50

# TR 1 - water maximum ([uL/L]{.underline}): 250

How is the dose administered? (**[X only one]{.underline}**)

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# TR 1 - Water static bath?: X

# TR 1 - Water flow-through?:

# TR 1 - For this regimen, on how many days would you administer treatment?: 3

# TR 1 - Are treatments administered on consecutive ([C]{.underline}) or alternate ([A]{.underline}) days?: A

How long does a typical treatment (exposure) last?
(**[minutes]{.underline}**)

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# TR 1 - Static - minimum: 15

# TR 1 - Static - maximum: 60

# TR 1 - Flow-through - minimum:

# TR 1 - Flow-through maximum:

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 1 - fungus: X

# TR 1 - BGD: X

# TR 1 - Columnaris / BCWD: X

# TR 1 - furunculosis / Aeromonas hydrophilia:

# TR 1 - BKD / ERM:

# TR 1 - trematodes, protozoans, or copepods: X

# TR 1 - other:

# If you checked TR 1 - other, [enter disease name]{.underline}:

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 1 - Coldwater: X

# TR 1 - Coolwater: X

# TR 1 - Warmwater: X

# Maximum number of culture units treated simultaneously

(Note - you entered culture unit size information beginning on page 10
{depending on printer})

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# TR 1 - egg jars size 1:

# TR 1 - egg jars size 2:

# TR 1 - heath stacks:

# TR 1 - clark-williams:

# TR 1 - tank size 1: 3

# TR 1 - tank size 2:

# TR 1 - tank size 3:

# TR 1 - raceway size 1: 1

# TR 1 - raceway size 2: 1

# TR 1 - raceway size 3:

# TR 1 - pond size 1:

# TR 1 - pond size 2:

# TR 1 - pond size 3:

# Maximum number of culture units treated on a typical day

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# TR 1 - egg jars size 1:

# TR 1 - egg jars size 2:

# TR 1 - heath stacks:

# TR 1 - clark-williams:

# TR 1 - tank size 1: 3

# TR 1 - tank size 2:

# TR 1 - tank size 3:

# TR 1 - raceway size 1: 1

# TR 1 - raceway size 2: 1

# TR 1 - raceway size 3:

# TR 1 - pond size 1:

# TR 1 - pond size 2:

# TR 1 - pond size 3:

Answer the following for tank/raceway/pond treatments.
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# TR 1 - What percent of the treated volume is drained from the culture unit after treatment? ([%]{.underline}): 50

# TR 1 - By what percent is the flow rate increased after treatment ([%]{.underline}): 0

# TR 1 - If flow rate is increased, how long is it maintained? ([min]{.underline}): 0

# How often would you typically administer this treatment regimen?

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# TR 1 - times per year ([enter number]{.underline}): 6

When do you typically treat? (**[X all that apply]{.underline}**)

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# TR 1 - spring:

# TR 1 - summer: X

# TR 1 - fall: X

# TR 1 - winter:

# Water-borne Chemical Treatment Regimen (TR) 2

Please select the chemical described in this treatment regimen (only one
chemical per treatment regimen description) and identify the life stage
treated by placing an **[E (eggs) or F (fish)]{.underline}** to the
right of the colon for the appropriate chemical.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - hydrogen peroxide: E

# TR 2 - chloramine-T:

# TR 2 - oxytetracycline:

# TR 2 - potassium permanganate:

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - water minimum ([mg/L]{.underline}):

# TR 2 - water maximum ([mg/L]{.underline}):

# TR 2 - water minimum ([uL/L]{.underline}): 500

# TR 2 - water maximum ([uL/L]{.underline}): 1000

How is the dose administered? (**[X only one]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - Water static bath?:

# TR 2 - Water flow-through?: X

# TR 2 - For this regimen, on how many days would you administer treatment?: 10

# TR 2 - Are treatments administered on consecutive ([C]{.underline}) or alternate ([A]{.underline}) days?: A

How long does a typical treatment (exposure) last?
(**[minutes]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - Static - minimum:

# TR 2 - Static - maximum:

# TR 2 - Flow-through - minimum: 15

# TR 2 - Flow-through maximum: 30

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - fungus: X

# TR 2 - BGD:

# TR 2 - Columnaris / BCWD:

# TR 2 - furunculosis / Aeromonas hydrophilia:

# TR 2 - BKD / ERM:

# TR 2 - trematodes, protozoans, or copepods:

# TR 2 - other:

# If you checked TR 2 - other, [enter disease name]{.underline}:

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - Coldwater: X

# TR 2 - Coolwater: X

# TR 2 - Warmwater: X

# Maximum number of culture units treated simultaneously

(Note - you entered culture unit size information beginning on page 10
{depending on printer})

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - egg jars size 1: 10

# TR 2 - egg jars size 2:

# TR 2 - heath stacks: 2

# TR 2 - clark-williams:

# TR 2 - tank size 1:

# TR 2 - tank size 2:

# TR 2 - tank size 3:

# TR 2 - raceway size 1:

# TR 2 - raceway size 2:

# TR 2 - raceway size 3:

# TR 2 - pond size 1:

# TR 2 - pond size 2:

# TR 2 - pond size 3:

# Maximum number of culture units treated on a typical day

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - egg jars size 1: 5

# TR 2 - egg jars size 2:

# TR 2 - heath stacks: 1

# TR 2 - clark-williams:

# TR 2 - tank size 1:

# TR 2 - tank size 2:

# TR 2 - tank size 3:

# TR 2 - raceway size 1:

# TR 2 - raceway size 2:

# TR 2 - raceway size 3:

# TR 2 - pond size 1:

# TR 2 - pond size 2:

# TR 2 - pond size 3:

Answer the following for tank/raceway/pond treatments.
\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - What percent of the treated volume is drained from the culture unit after treatment? ([%]{.underline}): 0

# TR 2 - By what percent is the flow rate increased after treatment ([%]{.underline}): 0

# TR 2 - If flow rate is increased, how long is it maintained? ([min]{.underline}): 0

# How often would you typically administer this treatment regimen?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - times per year ([enter number]{.underline}): 16

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 2 - spring: X

# TR 2 - summer:

# TR 2 - fall: X

# TR 2 - winter:

# Water-borne Chemical Treatment Regimen (TR) 3

Please select the chemical described in this treatment regimen (only one
chemical per treatment regimen description) and identify the life stage
treated by placing an **[E (eggs) or F (fish)]{.underline}** to the
right of the colon for the appropriate chemical.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - hydrogen peroxide:

# TR 3 - chloramine-T: F

# TR 3 - oxytetracycline:

# TR 3 - potassium permanganate:

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - water minimum ([mg/L]{.underline}): 10

# TR 3 - water maximum ([mg/L]{.underline}): 20

# TR 3 - water minimum ([uL/L]{.underline}):

# TR 3 - water maximum ([uL/L]{.underline}):

How is the dose administered? (**[X only one]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - Water static bath?:

# TR 3 - Water flow-through?: X

# TR 3 - For this regimen, on how many days would you administer treatment?: 4

# TR 3 - Are treatments administered on consecutive ([C]{.underline}) or alternate ([A]{.underline}) days?: C

How long does a typical treatment (exposure) last?
(**[minutes]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - Static - minimum:

# TR 3 - Static - maximum:

# TR 3 - Flow-through - minimum: 60

# TR 3 - Flow-through maximum: 60

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - fungus:

# TR 3 - BGD: X

# TR 3 - Columnaris / BCWD: X

# TR 3 - furunculosis / Aeromonas hydrophilia:

# TR 3 - BKD / ERM:

# TR 3 - trematodes, protozoans, or copepods:

# TR 3 - other:

# If you checked TR 3 - other, [enter disease name]{.underline}:

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - Coldwater: X

# TR 3 - Coolwater: X

# TR 3 - Warmwater: X

# Maximum number of culture units treated simultaneously

(Note - you entered culture unit size information beginning on page 10
{depending on printer})

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - egg jars size 1:

# TR 3 - egg jars size 2:

# TR 3 - heath stacks:

# TR 3 - clark-williams:

# TR 3 - tank size 1: 4

# TR 3 - tank size 2:

# TR 3 - tank size 3:

# TR 3 - raceway size 1: 2

# TR 3 - raceway size 2: 1

# TR 3 - raceway size 3:

# TR 3 - pond size 1:

# TR 3 - pond size 2:

# TR 3 - pond size 3:

# Maximum number of culture units treated on a typical day

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - egg jars size 1:

# TR 3 - egg jars size 2:

# TR 3 - heath stacks:

# TR 3 - clark-williams:

# TR 3 - tank size 1: 4

# TR 3 - tank size 2:

# TR 3 - tank size 3:

# TR 3 - raceway size 1: 2

# TR 3 - raceway size 2: 1

# TR 3 - raceway size 3:

# TR 3 - pond size 1:

# TR 3 - pond size 2:

# TR 3 - pond size 3:

Answer the following for tank/raceway/pond treatments.
\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - What percent of the treated volume is drained from the culture unit after treatment? ([%]{.underline}): 0

# TR 3 - By what percent is the flow rate increased after treatment ([%]{.underline}): 0

# TR 3 - If flow rate is increased, how long is it maintained? ([min]{.underline}): 0

# How often would you typically administer this treatment regimen?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - times per year ([enter number]{.underline}): 3

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 3 - spring:

# TR 3 - summer: X

# TR 3 - fall:

# **TR 3 - winter: **X

# Water-borne Chemical Treatment Regimen (TR) 4

Please select the chemical described in this treatment regimen (only one
chemical per treatment regimen description) and identify the life stage
treated by placing an **[E (eggs) or F (fish)]{.underline}** to the
right of the colon for the appropriate chemical.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - hydrogen peroxide:

# TR 4 - chloramine-T:

# TR 4 - oxytetracycline: F

# TR 4 - potassium permanganate:

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - water minimum ([mg/L]{.underline}): 10

# TR 4 - water maximum ([mg/L]{.underline}): 50

# TR 4 - water minimum ([uL/L]{.underline}):

# TR 4 - water maximum ([uL/L]{.underline}):

How is the dose administered? (**[X only one]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - Water static bath?: X

# TR 4 - Water flow-through?:

# TR 4 - For this regimen, on how many days would you administer treatment?: 3

# TR 4 - Are treatments administered on consecutive ([C]{.underline}) or alternate ([A]{.underline}) days?: C

How long does a typical treatment (exposure) last?
(**[minutes]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - Static - minimum: 60

# TR 4 - Static - maximum: 60

# TR 4 - Flow-through - minimum:

# TR 4 - Flow-through maximum:

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - fungus:

# TR 4 - BGD:

# TR 4 - Columnaris / BCWD: X

# TR 4 - furunculosis / Aeromonas hydrophilia: X

# TR 4 - BKD / ERM:

# TR 4 - trematodes, protozoans, or copepods:

# TR 4 - other: X

# If you checked TR 4 - other, [enter disease name]{.underline}: UNIDENTIFIED SYSTEMIC BACTERIA

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - Coldwater:

# TR 4 - Coolwater: X

# TR 4 - Warmwater: X

# Maximum number of culture units treated simultaneously

(Note - you entered culture unit size information beginning on page 10
{depending on printer})

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - egg jars size 1:

# TR 4 - egg jars size 2:

# TR 4 - heath stacks:

# TR 4 - clark-williams:

# TR 4 - tank size 1: 2

# TR 4 - tank size 2: 2

# TR 4 - tank size 3:

# TR 4 - raceway size 1:

# TR 4 - raceway size 2:

# TR 4 - raceway size 3:

# TR 4 - pond size 1:

# TR 4 - pond size 2:

# TR 4 - pond size 3:

# Maximum number of culture units treated on a typical day

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - egg jars size 1:

# TR 4 - egg jars size 2:

# TR 4 - heath stacks:

# TR 4 - clark-williams:

# TR 4 - tank size 1: 1

# TR 4 - tank size 2: 1

# TR 4 - tank size 3:

# TR 4 - raceway size 1:

# TR 4 - raceway size 2:

# TR 4 - raceway size 3:

# TR 4 - pond size 1:

# TR 4 - pond size 2:

# TR 4 - pond size 3:

Answer the following for tank/raceway/pond treatments.
\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - What percent of the treated volume is drained from the culture unit after treatment? ([%]{.underline}): 50

# TR 4 - By what percent is the flow rate increased after treatment ([%]{.underline}): 0

# TR 4 - If flow rate is increased, how long is it maintained? ([min]{.underline}): 0

# How often would you typically administer this treatment regimen?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - times per year ([enter number]{.underline}): 3

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 4 - spring: X

# TR 4 - summer: X

# TR 4 - fall:

# TR 4 - winter:

# Water-borne Chemical Treatment Regimen (TR) 5

Please select the chemical described in this treatment regimen (only one
chemical per treatment regimen description) and identify the life stage
treated by placing an **[E (eggs) or F (fish)]{.underline}** to the
right of the colon for the appropriate chemical.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - hydrogen peroxide:

# TR 5 - chloramine-T:

# TR 5 - oxytetracycline:

# TR 5 - potassium permanganate:

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - water minimum ([mg/L]{.underline}):

# TR 5 - water maximum ([mg/L]{.underline}):

# TR 5 - water minimum ([uL/L]{.underline}):

# TR 5 - water maximum ([uL/L]{.underline}):

How is the dose administered? (**[X only one]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - Water static bath?:

# TR 5 - Water flow-through?:

# TR 5 - For this regimen, on how many days would you administer treatment?:

# TR 5 - Are treatments administered on consecutive ([C]{.underline}) or alternate ([A]{.underline}) days?:

How long does a typical treatment (exposure) last?
(**[minutes]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - Static - minimum:

# TR 5 - Static - maximum:

# TR 5 - Flow-through - minimum:

# TR 5 - Flow-through maximum:

Disease treated (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - fungus:

# TR 5 - BGD:

# TR 5 - Columnaris / BCWD:

# TR 5 - furunculosis / Aeromonas hydrophilia:

# TR 5 - BKD / ERM:

# TR 5 - trematodes, protozoans, or copepods:

# TR 5 - other:

# If you checked TR 5 - other, [enter disease name]{.underline}:

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - Coldwater:

# TR 5 - Coolwater:

# TR 5 - Warmwater:

# Maximum number of culture units treated simultaneously

(Note - you entered culture unit size information beginning on page 10
{depending on printer})

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - egg jars size 1:

# TR 5 - egg jars size 2:

# TR 5 - heath stacks:

# TR 5 - clark-williams:

# TR 5 - tank size 1:

# TR 5 - tank size 2:

# TR 5 - tank size 3:

# TR 5 - raceway size 1:

# TR 5 - raceway size 2:

# TR 5 - raceway size 3:

# TR 5 - pond size 1:

# TR 5 - pond size 2:

# TR 5 - pond size 3:

# Maximum number of culture units treated on a typical day

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - egg jars size 1:

# TR 5 - egg jars size 2:

# TR 5 - heath stacks:

# TR 5 - clark-williams:

# TR 5 - tank size 1:

# TR 5 - tank size 2:

# TR 5 - tank size 3:

# TR 5 - raceway size 1:

# TR 5 - raceway size 2:

# TR 5 - raceway size 3:

# TR 5 - pond size 1:

# TR 5 - pond size 2:

# TR 5 - pond size 3:

Answer the following for tank/raceway/pond treatments.
\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - What percent of the treated volume is drained from the culture unit after treatment? ([%]{.underline}):

# TR 5 - By what percent is the flow rate increased after treatment ([%]{.underline}):

# TR 5 - If flow rate is increased, how long is it maintained? ([min]{.underline}):

# How often would you typically administer this treatment regimen?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - times per year ([enter number]{.underline}):

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# TR 5 - spring:

# TR 5 - summer:

# TR 5 - fall:

# TR 5 - winter:

# Water-borne Anesthetic Regimen (AR) 1 -- Aqui-S Use at Hatcheries

***anticipated dose - 25 to 50 mg/L***

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 1 - Coldwater: X

# AR 1 - Coolwater: X

# AR 1 - Warmwater: X

# What is the anesthesia purpose (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 1 - spawning: X

# AR 1 - tag/release/mark: X

# AR 1 - transportation:

# AR 1 - collection:

# AR 1 - other: X

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 1 - water minimum ([mg/L]{.underline}): 25

# AR 1 - water maximum ([mg/L]{.underline}): 50

# How is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 1 - On an annual basis, on how many days would you administer treatment?: 6

# AR 1 - What volume of anesthetic bath would you typically prepare? ([L]{.underline}): 120

# AR 1 - How many times per day would you prepare the above volume?: 3

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 1 - spring: X

# AR 1 - summer: X

# AR 1 - fall: X

# **AR 1 - winter: **X

*Water-borne Anesthetic Regimen (AR) 2 -- Aqui-S Use Away from the
Hatchery *

***anticipated dose - 25 to 50 mg/L***

# What types of fish are treated (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 2 - Coldwater:

# AR 2 - Coolwater:

# AR 2 - Warmwater:

# What is the anesthesia purpose (**[X all that apply]{.underline}**)?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 2 - spawning:

# AR 2 - tag/release/mark:

# AR 2 - transportation:

# AR 2 - collection:

# AR 2 - other:

# What is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 2 - water minimum ([mg/L]{.underline}):

# AR 2 - water maximum ([mg/L]{.underline}):

# How is the dose administered?

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 2 - On an annual basis, on how many days would you administer treatment?:

# AR 2 - What volume of anesthetic bath would you typically prepare? ([L]{.underline}):

# AR 2 - How many times per day would you prepare the above volume?:

When do you typically treat? (**[X all that apply]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

# AR 2 - spring:

# AR 2 - summer:

# AR 2 - fall:

# AR 2 - winter:

What type of water body is the anesthetic bath discharged to? (**[X only
one]{.underline}**)

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

**AR 2 - **Lake/Pond:

**AR 2 - **River/Stream:

**AR 2 - **Backwater of a River/Stream:

If the anesthetic enters a Lake/Pond, estimate the following.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

**AR 2 - **What is the estimated average volume?
(**[acre-feet]{.underline}**)?:

If the anesthetic enters a River/Stream, answer the following.

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

**AR 2 - **If River/Stream selected, what is the estimated average flow?
(**[cfs]{.underline}**):

**AR 2 - **The lowest flow occurs during what season? (**[NC if no
change]{.underline}**):

**AR 2 - **What is the estimated average flow during the low flow
season? (**[cfs]{.underline}**):

++++++++++

# END SURVEY