**[ACORP Appendix 4]{.underline}[ ]{.underline}**

**[Antemortem Specimen Collection]{.smallcaps}**

**[Version 3]{.smallcaps}**

[1. ]{.underline}**[Blood Collection.]{.underline}**[ Will blood be
collected from live animals (anesthetized or awake) as a part of this
proposal? ]{.underline}

[ No. Proceed to item 3. ]{.underline}

[ Yes, but all collections are described in Appendix 2, "Antibody
Production", so no further information need be provided here; proceed to
item 3. ]{.underline}

[ Yes. Complete the table below; then proceed to item 2. ]{.underline}

  --------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------- ------------------------------------------- --------------------------------------------
  [Site and Method of blood collection]{.underline}   [Amount of blood collected, expressed as volume (ml) and % of body weight (assume 1 ml of blood weighs 1 gram)]{.underline}   [Number of blood collections]{.underline}   [Interval between collections]{.underline}
                                                                                                                                                                                                                                
                                                                                                                                                                                                                                
                                                                                                                                                                                                                                
                                                                                                                                                                                                                                
                                                                                                                                                                                                                                
                                                                                                                                                                                                                                
  --------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------- ------------------------------------------- --------------------------------------------

[2. ]{.underline}**[Use of Anesthetics, Tranquilizers, or Analgesics for
Blood Collection]{.underline}**[. Will anesthetics, tranquilizers, or
analgesics be used to prevent pain or stress during collection of blood
described in item 1 above? ]{.underline}

[ No. [Justify the omission of pain-relieving agents]{#Check4}[ (either
scientifically or because the collection method involves no or momentary
pain) and ]{#Check4}[completely describe the physical
restraint]{#Check4}[ that will be used during collection here:
]{#Check4}]{.underline}

[ Yes. Complete the following table, then proceed to item 3.
]{.underline}

+----------------+----------------+----------------+----------------+
| [Anesthetic,   | [Dose (mg/kg)  | # [Rout        | ## [Frequenc   |
| tranquilizer,  | & volume       | e]{.underline} | y]{.underline} |
| or analgesic   | (ml            |                |                |
| agen           | )]{.underline} |                |                |
| t]{.underline} |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+

[3. ]{.underline}**[Other Tissue Collection.]{.underline}**[ Will other
body fluids (e.g. cerebrospinal fluid, peritoneal fluid, urine) or
tissues be collected from live animals (awake or anesthetized) as a part
of this protocol? ]{.underline}

[ No. Proceed to item 5. ]{.underline}

[ Yes. Complete the following table; then proceed to item 4.
]{.underline}

  ----------------------------------------- ------------------------------------------- ----------------------------------------- ------------------------------------- --------------------------------------------
  [Tissue or fluid collected]{.underline}   [Site & method of collection]{.underline}   [Amount (g) or volume (ml)]{.underline}   [Number of collections]{.underline}   [Interval between collections]{.underline}
                                                                                                                                                                        
                                                                                                                                                                        
                                                                                                                                                                        
                                                                                                                                                                        
                                                                                                                                                                        
                                                                                                                                                                        
  ----------------------------------------- ------------------------------------------- ----------------------------------------- ------------------------------------- --------------------------------------------

[4. ]{.underline}**[Use of]{.underline}**[ ]{.underline}**[Anesthetics,
Tranquilizers, or Analgesics for Collection of Fluids or
Tissues]{.underline}**[. Will anesthetics, tranquilizers, or analgesics
be used to prevent pain or stress during collection of body fluids or
tissues described in item 3 above? ]{.underline}

[ No. [Justify the omission of pain-relieving agents]{#Check8}[ (either
scientifically or because the collection method involves no or momentary
pain) and ]{#Check8}[completely describe the physical
restraint]{#Check8}[ that will be used during collection here, then go
to ite]{#Check8}[m 5:]{#Check8}]{.underline}

[ Yes. Complete the following table, then go to item 5. ]{.underline}

+----------------+----------------+----------------+----------------+
| [Anesthetic,   | [Dose (mg/kg)  | # [Rout        | ## [Frequenc   |
| tranquilizer,  | & volume       | e]{.underline} | y]{.underline} |
| or analgesic   | (ml            |                |                |
| agen           | )]{.underline} |                |                |
| t]{.underline} |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+
|                |                |                |                |
+----------------+----------------+----------------+----------------+

[5. Proceed to item S on the ACORP. ]{.underline}