# Presentation: 242090

## DOE Genomics:GTL Roadmap

**BERAC Advisory Committee Meeting**
**April 30, 2004**
**John Houghton**
**John.Houghton@science.doe.gov**

## Energy Bill (not passed) Requires Roadmap

*(c) PLAN*
*(1) DEVELOPMENT OF PLAN.—Not later than 1 year after the date of enactment of this Act, the Secretary shall prepare and transmit to Congress a research plan describing how the program authorized pursuant to this section will be undertaken to accomplish the program goals established in subsection (b). *
*(2) REVIEW OF PLAN.—The Secretary shall contract with the National Academy of Sciences to review the research plan developed under this sub-section. The Secretary shall transmit the review to Congress not later than 18 months after transmittal of the research plan under paragraph (1), along with the Secretary’s response to the recommendations contained in the review.*
- NSF recently advised to make more use of roadmaps for facilities

## Roadmap Outline

**Executive Summary**:
**Introduction**;
***Vision**:  **Frazier**
**Science and the Research Plan**:
**Facilities**:
**Computation**:
***Environmental Remediation****:  ****Fryberger, Bolton, Hochella**** **
***Energy**: **Houghton, Davison, Siebert**
***Terrestrial sequestration and climate science**: **Amthor, Rogers**
***Ocean sequestration and climate science**:  **Drell, Armbrust**
**Ancillary Benefits**:  **basic science, unforeseen events, etc.**
**ELSI**:
**Governance**:
*** ** breakout session attached to GTL meeting March 3,4

## 1. Problem Statement: The problem that exists and the solution that is sought.

**2.** **Vision for 20XX: **

**3. ****Mission End Points**
**And  Requirements**

**4. ****Today’s State of**
**The Art in Knowledge **
**And Capabilities**

**5. ****Major Milestones in **
**Science**** and ****Capabilities**

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**March 3 Template for Functional Roadmap – In sequence**

## 1. Problem Statement: The problem that exists and the solution that is sought.

**2.** **Vision for 20XX: **

**3. ****Mission End Points**
**And  Requirements**

**4. ****Today’s State of**
**The Art in Knowledge **
**And Capabilities**

**5. ****Major Milestones in **
**Science**** and ****Capabilities**

**.........****.**
**.........**
**......****.**
**...****..**
**...**
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**March 3 Template for Functional Roadmap – In sequence**

**March 4: Delineate Technologies and Computing**

**T**

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**C**

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**C**

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## PHOTOSYNTHETIC PATHWAYS (light reactions)

**O****2**

**2H****2****O**

**PS II**

**PQ-pool**

**PS I**

**Fd**

**H****2****ase**

**H****2**

- e-

- e-

**Chl antenna**

**Chl antenna**

**CO****2****        starch**

- Sun

**H+**

**H****+**

**H+**

- ADP + P       ATP

**H+**

- INSIDE

- OUTSIDE

- MEMBRANE

- ANTENNA

## Basic Plant Research

- ORNL WSC24335

**Carbon Fixation**

**and Sequestration**

**Agriculture Land**

**Forest Land**

**Wetlands**

**Degraded Lands**

**Liquid Fuels**

**Biogas**

**Biopower**

**Biohydrogen**

**Bioenergy and Carbon Cycling**

**Biopolymers and**

**Plastics**

**Food, Feed, and Fiber**

**Specialty Products**

**Bioproducts and Carbon Cycling**

**Basic Plant Research**

**Water**

**Management**

**Photosynthetic**

**Efficiency**

**Nutrient**

**Use**

**Efficiency**

**Cell Wall**

**Assembly**

**COFixation**

**2**

** **

**Genomics and**

**Gene Expression**

## Selected Clean Energy Capabilities

- Protein production
- Improved heterologous expression.
- Suite of heterologous expession hosts (i.e., control the redox state, codon usage, small molecule partners, and recombinant production of metal-containing proteins).
- Assays
- Measure target metabolite* in situ *(# per cell).
- Design assays to match cellular milieu.
- Activity-based assays.
- Rational design of molecular machines
- Survey diversity of hydrogenases or glycosyl hydrolyses.
- Full characterization of partners, energetics, structures, PTMs, etc.
- Computation tools for rational design/recombination.

## Selected Clean Energy Technology Requirements

***Imaging Needs:***
***Single Molecule Sensitivity***
***Molecular to >cm scale, 3-D resolution***
***High sensitivity and spatial resolution MR including phosphorus and 13C***
***X-Ray and Electron based microscopies***
***High sensitivity optical with enhanced spatial resolution***
***Imaging of opaque samples***
***The ability to  make multiple assays simultaneously***
***Combined scanning probe (AFM, STM, nano-sensor) and optical imaging***
***Simultaneous use of multiple optical probes***
***Two/Three Photon, CARS, High-Speed, SERS, FRET, FLASH, etc.***
***Simultaneous MR/Confocal and Scanning-MS/Confocal***
***Real-time, 3-D measures of time and spatial distributions***
***Redox potential, protein & metabolite concentrations, lipids, energetic species, cofactors, etc.***
***Molecular probes***
***Highly sensitive, multiple analytes, functionally sensitive, inert, and accessible to the cell interior.***
***Affordable and accessible Tools***
***User facilities should foster the development and export of easily usable versions of new technologies.***

## Products of Technology Deep Dive Specifications Workshop 

- Science Driven Capabilities specifications
- Analysis of technologies specifications related to identified capabilities – identifying strengths, weaknesses, and gaps
- Roadmaps for development of technologies to required performance
- Analysis of computing needs of science, capabilities, and technologies
- Roadmaps for computing environment development
- Analysis of natural groupings or suites of technologies which might drive facilities specifications.