# Presentation: 916362

## 5th National Symposium on Biosafety: A Rational Basis for Biocontainment

**Biosafety in Microbiological****and Biomedical Laboratories****3****rd**** Edition****JY Richmond & RW McKinney ****(eds.)**

## BMBL Introduction

**1941 - Meyer and Eddie**
- 74 lab associated brucellosis infections in US
**1949 - Sulkin and Pike**
- 222 viral infections (21 fatal)
- Only 27% related to known accidents

**Notes:**
In this series of 4 introduction slides, we are working on a more “human interest” focus to cover the background for the BMBL.
Suggestions were:
	Photos of researchers
	Identify researchers area of expertise

Any suggestions?

JYR: Suggested stock photo on cover of APHA book. Check with Richard to get it.

## BMBL Introduction

**1951,1965, 1976 - Sulkin and Pike**
- Surveys for lab-associated infections
- More than 5,000 labs
- Cumulative total of 3,921cases cited
- Most commonly reported:
- Hepatitis		 Brucellosis
- Tuberculosis	 Tularemia
- Typhoid
- Venzuelan Equine Encephalitis

**Notes:**

JYR: Look for very old slide that shows the type of  infectious agents (by time period) that were responsible for Lab Acquired Infections. Could be redone.	

## BMBL Introduction

**1951,1965, 1976 - Sulkin and Pike** (cont.)
- Surveys for lab-associated infections
- Fewer than 20% associated with known accidents
- Exposure to infectious aerosols plausible (but unconfirmed) for >80% of reported cases

## Principles of BiosafetyIntroduction

**Biosafety Levels 1-3**
- Guidelines to describe combinations of:
- Laboratory Practices and Techniques
- Standard Practices
- Special Practices
- Safety Equipment (Primary Barriers)
- Laboratory Facilities (Secondary Barriers)

**Notes:**
The slide Before or After this intro slide would be the graphic that has 3 concentric circles depicting Practices, Safety Equipment, Facilities.
JYR: YES!

Since this is a talk on BSL 1-3, should this slide have title of 
Biosafety Levels 1-3 

## Principles of Biosafety Introduction

**Biosafety Levels 1-3 Provide**
- Increasing levels of personnel and environmental protection
- Guidelines for working safely in microbiological and biomedical laboratories

## Lab Practices and Techniques Introduction

- Knowledgeable supervisor
- Personnel
- Aware of potential hazards
- Proficient in practices/techniques
- Biosafety manual specific to lab

**Notes:**
Graphics Ideas:
	Notebook (no) or picture of manual (yes)
	Person at BSC with appropriate dress

## Safety Equipment (Primary Barriers) - Introduction

- Biosafety cabinets (BSCs) [BSL-2/3]
- Personal protective clothing
- Gloves
- Gowns
- Pipetting Devices
- Safety centrifuge cups and rotors
- Eye and face protection
- Respiratory protection [BSL-3]

**Notes:**
Graphics Ideas:
	Back of BMBL has line drawings (p 142-45) of BSC
	Picture or drawing of Eye Protection
	Centrifuge Cups

JYR: For the intro, since these are additive, highlight gloves, gowns and pipetting devices.

(I omitted >> Safety centrifuge cups and rotors << due to space limitations. Could add if necessary.)

## Biosafety Level 1Introduction

- Suitable for work involving well-characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.

## Biosafety Level 1 Introduction

- Examples:
*Bacillus subtilis*
*Naegleria gruberi*
- Infectious canine hepatitis virus
*E. coli*

## Biosafety Level 1 Laboratory Facilities (Secondary Barriers)

## Biosafety Level 1 Laboratory Facilities (Secondary Barriers)

- Sink for handwashing
- Work surfaces easily cleaned
- Bench tops
- Sturdy furniture
- Windows fitted with flyscreens