# Presentation: 174843

## Howard Rines1, Hedera Porter1,
Marty Carson2, and Jerry Ochocki2

1USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota
2USDA-ARS Cereal Disease Lab, and Dept. of Plant Pathology, Univ. of Minnesota

- Marty Carson2, and Jerry Ochocki2
- 1USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota
- 2USDA-ARS Cereal Disease Lab, and Dept. of Plant Pathology, Univ. of Minnesota

- Introgression of Oat Crown Rust Resistance from Diploid *Avena strigosa* and Tetraploid *Avena murphyi*

## Crown Rust on Oat

**Crown Rust on Oat**

## Crown rust of oat

- Resistance
- 		Past – major genes from *A. sterilis* (6x),
- 			relatively short-lived effectiveness

## Crown rust of oat

-  Resistance
- 		Past – major genes from *A. sterilis* (6x),
- 			relatively short-lived effectiveness
- 		Current – *Pc94* from *A. strigosa* (2x),
- 			     only effective single gene

## Crown rust of oat

-  Resistance
- 		Past – major genes from *A. sterilis* (6x),
- 			relatively short-lived effectiveness
- 		Current – *Pc94* from *A. strigosa* (2x),
- 			     only effective single gene
- 		Alternatives –Major gene combinations
- 				Race non-specific – partial, adult plant, 					           multigenic

## Crown rust of oat

- Resistance
- 		Past – major genes from *A. sterilis* (6x),
- 			relatively short-lived effectiveness
- 		Current – *Pc94* from *A. strigosa* (2x),
- 			     only effective single gene
- 		Alternatives – Major gene combinations
- 				  Race non-specific – partial, adult plant, 		      				  multigenic
- 		New sources – *A. strigosa* (2x) – CI 6954SP
- 			              *A. murphyi* (4x) – P12

## Rust reaction of the parental lines.

***A. strigosa***** CI 6954 ****	****	****	****	****	****HR**
***A. murphyi***** P12****	****	****	****	****	****HR**
***A. sativa***** cv. Ogle****	****	****	****	****	****S**
***A. sativa***** cv. Black Mesdag (BM)****	****	****	****S**

**Rust reaction of the parental lines.**

- Cross 1

**Notes:**
What are the similarities in resistance patterns between Pc94 derived lines and A. strigosa CI 6954SP?

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S
- 			colchicine
-   			fertile sectors self-pollinate
-  		          C1
- 		(2n=2x + 6x=56)
- 		            S

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S
- 			colchicine
-   			fertile sectors self-pollinate
-  		          C1
- 		(2n=2x + 6x=56)    X    *A. sativa* cv. Ogle
- 		            S		          (2n=6x=42)
- 					S
-                                                             BC1F1
-  	partial fertile self,	      (1x + 3x + 3x)
- 	highly fertile female        (2n=7x=49)
- 			                S

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S
- 			colchicine
-   			fertile sectors self-pollinate
-  		          C1
- 		(2n=2x + 6x=56)    X    *A. sativa* cv. Ogle
- 		            S		          (2n=6x=42)
- 					S
-                                                             BC1F1
-  	partial fertile self,	      (1x + 3x + 3x)   X    *A. sativa* cv. Ogle
- 	highly fertile female        (2n=7x=49)	           (2n=6x=42)
- 			                S		    S
- 	       			              BC2F1
- 	 			       (2n=6x + 0-7)
- 	 		 	           18R, 62S

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S
- 			colchicine
-   			fertile sectors self-pollinate
-  		          C1
- 		(2n=2x + 6x=56)    X    *A. sativa* cv. Ogle
- 		            S		          (2n=6x=42)
- 					S
-                                                             BC1F1
-  	partial fertile self,	      (1x + 3x + 3x)    X    *A. sativa* cv. Ogle
- 	highly fertile female       (2n=7x=49)	            (2n=6x=42)
- 			                S		    S
- 	       			              BC2F1
- 	       BC1F2 		       (2n=6x + 0-7)
- 	 (2n=6x + 0-7)	 	           18R, 62S
- 	   27R, 130S

**X**

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          (2n=2x=14)		         (2n=6x=42)
-                  HR        embryo     rescue	 S
- 		          F1
- 		(2n=1x + 3x=28)
- 		           S
- 			colchicine
-   			fertile sectors self-pollinate
-  		          C1
- 		(2n=2x + 6x=56)    X    *A. sativa* cv. Ogle
- 		            S		          (2n=6x=42)
- 					S
-                                                             BC1F1
-  	partial fertile self,	      (1x + 3x + 3x)    X    *A. sativa* cv. Ogle
- 	highly fertile female       (2n=7x=49)	            (2n=6x=42)
- 			                S		    S
- 	       			              BC2F1              X         *A. sativa* cv. Ogle
- 	       BC1F2 		       (2n=6x + 0-7)                  (2n=6x=42)
- 	 (2n=6x + 0-7)	 	           18R, 62S	  	     S
- 	   27R, 130S 			               BC3F1
- 					          (2n=6x + 0–7)
- 					            157R, 170S

**X**

## Possible interpretations

- Suppressor in Black Mesdag
- segregates out in BC1F2, BC2F1, and BC3F1 progeny

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

-          **(2n=2x=14)****	****	****         (2n=6x=42)**
-                 ** ****HR**        embryo     rescue	 **S**
- 		          **F****1**
**	****	****(2n=1x + 3x=28)**** **
**	****	****           ****S**
- 			colchicine
-   			fertile sectors self-pollinate
-  		          **C****1**
**	****	****(2n=2x + 6x=56)**    X    ***A. sativa***** cv. Ogle**
- 		            S		          **(2n=6x=42)**
- 					S
-                                                             **BC****1****F****1**
-  	partial fertile self,**	****      ****(1x + 3x + 3x)    ****X ****   *****A. sativa***** cv. Ogle**
- 	highly fertile female       **(2n=7x=49)****	****            (2n=6x=42)**
- 			                S		    S
- 	       			              **BC****2****F****1             **** **
**	****       ****BC****1****F****2**** ****	****	****       (2n=6x + 0-7)**
**	**** ****(2n=6x + 0-7)****	**** ****	****           ****18R, 62S****	****  ****	****     **
**	****   ****27R, 130S**** ****	****	****	**

**X**

## Possible interpretations

- Suppressor in Black Mesdag
- segregates out in BC1F2, BC2F1, and BC3F1 progeny
- Precedent:
*Pc62* (from *A. sterilis*) suppressed by *Pc38*
*Pc94* (from *A. strigosa*) suppressed by *Pc38*

## Possible interpretations

- Suppressor in Black Mesdag
- segregates out in BC1F2, BC2F1, and BC3F1 progeny
- Precedent:
*Pc62* (from *A. sterilis*) suppressed by *Pc38*
*Pc94* (from *A. strigosa*) suppressed by *Pc38*
- Tested:
*Pc94* x Black Mesdag  ---  R

## Possible interpretations

- Suppressor in Black Mesdag
- segregates out in BC1F2, BC2F1, and BC3F1 progeny
- Precedent:
*Pc62* (from *A. sterilis*) suppressed by *Pc38*
*Pc94* (from *A. strigosa*) suppressed by *Pc38*
- Tested:
*Pc94* x Black Mesdag  ---  R
- Tested:
- New R x Black Mesdag
- New R x *Pc38*

## Test cross of resistant 

**	****	****	****	****	****     ****Pc 38****	****Jerry ****	****   Black**
**	****	****	****	**_**	**__**	**__**	**__**(Pc 38)**__**	**__**  Mesdag**_
**	****	****	****	****	****      **_**R   S**_**       ****	**** **_**R   S**_**         **_**R   S**_
**	****	****	****	****	****      ****-     -****	**** -    -****	****       ***
**	****	****	****	****	****      ****0    6****	**** 0   7****	****       ***
**	****	****	****	****	****      ****0    2****	****  -   -****	****       ***
**	****	****	****	****	****      ****0  10****	****  -   -****	****       ***
**	****	****	****	****	****      ****0    2****	**** 0   6****	****       ***
**	****	****	****	****	****      ****0    7****	**** 0   5****	****       ***
**Pc 94****	****	****	****	****	****      0    4****	**** 0   11      R,MR**

**	****	****	****	**_**	**__**	**__**Cross**__**	**__**	**__**	**_
**	****	****	****	****Ogle-c****	**
**	****	****	****	**
**	****	****	****	**_**R   S**_**	****      **
**	****	****	****	****7   0****	****	****	**
**	****	****	****	****8   1****	****      ****	**
**	****	****	****	****6   3****	****      ****	****	**
**	****	****	****	****3   7****	****      ****	**
**	****	****	****	****7   0****	****      ****	**
**	****	****	****	****8   0****	**

**	****	****	****	**
_**Plant No.**_**	**_**Self**_**	****	**
**	**
**	****	**_**R   S**_**	****	**
**12(3) - 1****	****-     -****	****	****	****	**
**12(3) - 3****	****10  0****	****	****	**
**20(10) - 1****	****-      -****	****	****	****	**
**20(10) - 3****	**** 9   1****	**** ****	****	**
**24(3) - 1****	**** 7   0****	****	**
**24(3) - 2****	**** 9   0****	****	**
**	**
**Inoculated at seedling stage with crown rust.**

**Test cross of resistant **
***A.***** *****strigosa*****/Black Mesdag BC****2****F****2**

***crosses with Black Mesdag**
**   ****35R  47S   2MR/R   3RSP**

## Transmission of Introduced Resistance

- Past efforts with *A. strigosa* sources
- 	1. Forsberg and co-workers
- Resistance present on an alien addition or substitution chromosome, only female transmission
- Used gamma irradiation treatment
- 2. Aung and Chong
- --	Resistance on an alien addition chromosome, unstable transmission
- -- Induced non-homologous pairing using crosses to *A. longiglumis* CW57

## Transmission of Introduced Resistance

- Past efforts with *A. strigosa* sources
- 	1. Forsberg and co-workers
- Resistance present on an alien addition or substitution chromosome, only female transmission
- Used gamma irradiation treatment
- 2. Aung and Chong
- --	Resistance on an alien addition chromosome, unstable transmission
- -- Induced non-homologous pairing using crosses to *A. longiglumis* CW57

##    A. strigosa CI6954SP     X    A. sativa cv. Black Mesdag

**         ****(2n=2x=14)****	****	****         (2n=6x=42)**** **
**                 ****HR****        embryo     ****rescue****	**** S**
**	****	****          ****F****1**
**	****	****(2n=1x + 3x=28)**** **
**	****	****           ****S**
**	****	****	****colchicine**
**  ****	****	****	****fertile sectors self-pollinate**
** ****	****	****          ****C****1**
**	****	****(2n=2x + 6x=56)    ****X****    *****A. sativa***** cv. Ogle**
**	****	****           **** ****S****	****	****          ****(2n=6x=42)**
**	****	****	****	****	****S**
**                                                            ****BC****1****F****1**
** ****	****partial fertile self,****	****      ****(1x + 3x + 3x)    ****X ****   *****A. sativa***** cv. Ogle**
**	****highly fertile female       ****(2n=7x=49)****	****            (2n=6x=42)**
**	****	****	****               **** ****S****	****	****    ****S**
**	****       ****	****	****	****              ****BC****2****F****1             **** ****X****        **** *****A. sativa***** cv. Ogle**
**	****       ****BC****1****F****2**** ****	****	****       (2n=6x + 0-7)                  (2n=6x=42)**
**	**** ****(2n=6x + 0-7)****	**** ****	****           ****18R, 62S****	****  ****	****     S**
**	****   ****27R, 130S**** ****	****	****	****               ****BC****3****F****1**
**	****	****	****	****	****          ****(2n=6x + 0–7)**
**	****	****	****	****	****            ****157R, 170S**

**X**

## Reciprocal crosses to Ogle-C of resistant A. strigosa CI 6954SP BC3 derivatives as:

- _BC3 plant_             _    female    _            _     male     _       _
- 				 _S_     _R_    _MR_            _S _    _R_    _MR_
- 12(1)-3			  4	3	0	        1	     1          0
- 12(3)-12		  4	6	0	        6        4          0
- 15(6)-8			  3	6	7	        2        1      	    5
- 20(5)-19		  5	3	0	        2        4          0
- 20(7)-6			  4	7	1	        4        3          1
- 20(7)-16		  7    	5	2	        5        4          1
- 20(10)-7		  3	2	0	        5        8          0
- 20(10)-17		  3	6	1	        1        3          0
- 20(10)-20		  5	5	0	        7        3          0
- 20(12)-1		  2	2	6	        3        7          0
- 20(12)-9		  _7_	_3_	_5_	        _7_        _8 _         _0_
- Total			 47      48          22                 43       46        7

## Buckthorn nursery test of resistant A. strigosa BC3F2 

_**Plant No.**_**	**_**R**_**	****	**_**S**_**	****	**_**R/S**_
- 12(1)-1		39		16		2.4
- 12(1)-7		54		14		3.9
- 12(3)-5		56		15		3.7
- 15(6)-19		  2		26		0.1
- 18(5)-4		41		13		3.2
- 18(5)-10		52		19		2.7
- 20(7)-6		51		15		3.4
- 21(6)-6		43		  5		8.6
- 23(4)-15		18		  0		N/A

- Cross 2

**Notes:**
What are the similarities in resistance patterns between Pc94 derived lines and A. strigosa CI 6954SP?

##    A. strigosa CI6954SP     X     A. murphyi P12

*   ****A. strigosa***** CI6954SP     ****X****     *****A. murphyi***** P12**
**            ****(2n=2x=14)****	****	****      (2n=4x=28)**** **
**                    ****HR****          colchicine          ****HR**
**	****	****	****      ****C****1****	**
**	****	****       ****(2n=2x + 4x=42)**
**	****	****	****      ****MR****	**

##    A. strigosa CI6954SP     X     A. murphyi P12

*   ****A. strigosa***** CI6954SP     ****X****     *****A. murphyi***** P12**
**            ****(2n=2x=14)****	****	****      (2n=4x=28)**** **
**                    ****HR****          colchicine          ****HR**
**	****	****	****      ****C****1****	****           ****X ****     *****A. sativa***** cv. Ogle**
**	****	****       ****(2n=2x + 4x=42)              (2n=6x=42)**
**	****	****	****      ****MR****	****	****         ****S**
**	****	****	****                   ****BC****1****F****1**
** ****	****	****                  ****(2n=1x + 2x +3X=42)    **
**	****	****	**** ****	****           ****S****	**

##    A. strigosa CI6954SP     X     A. murphyi P12

***   ******A. strigosa***** CI6954SP     ****X****     *****A. murphyi***** P12**
**            ****(2n=2x=14)****	****	****      (2n=4x=28)**** **
**                    ****HR****          colchicine          ****HR**
**	****	****	****      ****C****1****	****             ****X****      *****A. sativa***** cv. Ogle**
**	****	****       ****(2n=2x + 4x=42)               (2n=6x=42)**
**	****	****	****      ****MR****	****	****          ****S**
**	****	****	****	**
**	****	****	****                    ****BC****1****F****1             ****X    *****A. sativa *****cv. Ogle***** ***
** ****	****	****                   ****(2n=1x+2x+3X=42)      **** ****(2n=6x=42)**
***	******	******	******	*****       ****S****	****	****	****         S**
**	****	****	****	**
**	****	****       ****	****	****                ****        ****BC****2****F****1**** ****	**
**	****	****    ****	****	****	****           ****(2n=?)**
**	****	****	****	****	**** ****     ****R,MR,MS,S**
**	****       ****	****	**

## Summary

- Oat crown rust resistance in accessions of        *A.* *strigosa* (2x) and *A. murphyi* (4x)

## Summary

- Oat crown rust resistance in accessions of        *A.* *strigosa* (2x) and *A. murphyi* (4x)
- Resistance suppressed in early generation crosses with *A. sativa*, but resistant segregants in later generations

## Summary

- Oat crown rust resistance in accessions of        *A.* *strigosa* (2x) and *A. murphyi* (4x)
- Resistance suppressed in early generation crosses with *A. sativa*, but resistant segregants in later generations
- Resistance is transmitted equally via male or female.  In most cases, transmission appears stable.

## Summary

- Oat crown rust resistance in accessions of        *A.* *strigosa* (2x) and *A. murphyi* (4x)
- Resistance suppressed in early generation crosses with *A. sativa*, but resistant segregants in later generations
- Resistance is transmitted equally via male or female.  In most cases, transmission appears stable.
- Preliminary segregation data suggest most lines have a single major gene.

## Summary

- Oat crown rust resistance in accessions of        *A.* *strigosa* (2x) and *A. murphyi* (4x)
- Resistance suppressed in early generation crosses with *A. sativa*, but resistant segregants in later generations
- Resistance is transmitted equally via male or female.  In most cases, transmission appears stable.
- Preliminary segregation data suggest most lines have a single major gene.
- Hypersensitive response of many plants further support single major gene.

**Notes:**
ACTUALLY this doesn’t apply to the work with Ogle x A. murphyi    CHANGE

- Cross 3

**Notes:**
What are the similarities in resistance patterns between Pc94 derived lines and A. strigosa CI 6954SP?

##      A. sativa cv. Ogle  X  A. murphyi P12

**            ****(2n=6x=42)****	****      (2n=4x=28)**** **
**                     ****S****                           HR**
**	****                         ****F****1**
**	****	****  ****(2n=3x + 2x=35)**** **
**	**** ****	****         ****MS, MR**
**	****	****	**

##      A. sativa cv. Ogle  X  A. murphyi P12

**            ****(2n=6x=42)****	****      (2n=4x=28)**** **
**                     ****S****                           HR**
**	****                         ****F****1**
**	****	****  ****(2n=3x + 2x=35)**** **
**	**** ****	****          ****MS, MR**
**	****	****	****             ****colchicine**
**  ****	****	****	****               ****fertile sectors self-pollinate**
**           ****F****2****	****	****               ****C****1**
**      ****(?x + ?x)****	****	****          (6x + 4x)**
**         ****2n = ?****	****                   ****(2n=10x=70)**** **
**	****S****	****                            S****	****	**

**X**

**Notes:**
NEED TO CORRECT

##      A. sativa cv. Ogle  X  A. murphyi P12

**            ****(2n=6x=42)****	****      (2n=4x=28)**** **
**                     ****S****                           HR**
**	****                         ****F****1**
**	****	****  ****(2n=3x + 2x=35)**** **
**	**** ****                     ****MS, MR**
**	****	****	****             ****colchicine**
**  ****	****	****	****               ****fertile sectors self-pollinate**
**           ****F****2****	****	****               ****C****1**
**      ****(?x + ?x)****	****	****          (6x + 4x)     ****X****    *****A. sativa***** cv. Ogle**
**         ****2n = ?****	****                   ****(2n=10x=70)****             (2n=6x=42)**
**	****S****	****                            S****	****	****                 ****S**
**                                                               ****BC****1****F****1**
** ****	**** ****	****                            ****(2n= 6x + 2x =56)**
**	****	****	****                             ****S****	****	****	**

**X**

##      A. sativa cv. Ogle  X  A. murphyi P12

-             (2n=6x=42)	      (2n=4x=28)
-                      S                           HR
- 	                         F1
- 		  (2n=3x + 2x=35)
- 	 	          MS,  MR
- 			             colchicine
-   			               fertile sectors self-pollinate
-            F2		               C1
-       (?x + ?x)		          (6x + 4x)     X    *A. sativa* cv. Ogle
-          2n = ?	                   (2n=10x=70)             (2n=6x=42)
- 	S	                            S		                 S
-                                                                BC1F1       X     *A. sativa* cv. Ogle
-  	 	                         (2n= 6x + 2x =56)           (2n=6x=42)
- 			                             S			  S
- 				                              BC2F1
- 	         	                        		(2n=6x + 0-7)
- 	         	 	 	                                  S

**X**

##      A. sativa cv. Ogle  X  A. murphyi P12

-             (2n=6x=42)	      (2n=4x=28)
-                      S                           HR
- 	                         **F****1**
- 		  (2n=3x + 2x=35)
- 	 	          MS,  MR
- 			             colchicine
-   			               fertile sectors self-pollinate
-            **F****2**		             **C****1**
-       (?x + ?x)		        (6x + 4x)      X    *A. sativa* cv. Ogle
-          2n = ?	                 (2n=10x=70)             (2n=6x=42)
- 	S	                            S		                 S
-                                                                **BC****1****F****1**      X     *A. sativa* cv. Ogle
-  	 	                         (2n= 6x + 2x =56)           (2n=6x=42)
- 			                            S			  S
- 	        **BC****1****F****2****	****	****                              ****BC****2****F****1             ****  **
**	****(2n=6x + 2x=56)         ****	****           (2n=6x + 0-7)**
**	****        ****S, MS****	**** ****	****                                  S**

**X**

**X**

## Slide 41