# Presentation: 939584

## Allergenic Products Advisory Committee, March 15, 2002

*Lab overview*
*Research update*
*Site visit summary*
*Rabin’s projects*
*Particulates update*
*compliance update*
*TSE update*
*Recombinant allergens*
*Glycerol*

## Laboratory of Immunobiochemistry

*Operational issues*

*Principal Investigators*
*Jay E. Slater, MD, Lab Chief – Supervisory Medical Officer  (3)*
*Ronald Rabin, MD - Senior Staff Fellow (<1)*
*Lyudmila Soldatova, PhD - Visiting Scientist (4)*
* *
*Post Doctoral Fellows*
*Jonny Finlay, PhD - IRTA (1)*
*Hui Huang, MD - Visiting Associate (<1)*
* *

*Research Technicians*
*Melissa Patterson- Biologist (2)*
*Albert Gam – Biologist (1)*
*Mona Febus – Microbiologist (2)*
*Marc Alston – Biologist  (1)*
*Cherry Valerio – Biologist (1)*
*Katia Dobrovolskaia - IRTA (<1)*
* *
*Fellows/Guest Workers*
*Bhavini Trivedi, MD - NIH Fellow (<1)*
*Gerry Poley, MD – Guest Worker (3)*
*Li-Shan Hsieh, PhD – Guest Worker (3)*

## LIB staffing 1998-2002

## “Routine” regulatory activities

*Lot release*
*Reference development*
*Reference distribution*
*Reference maintenance*
*semiannual checks*
*replacement*

*Lot release activities** *
*406 protocols submitted and reviewed*
*2 withdrawn*
*Reference distribution*
*2001: 2151 vials in 101 shipments sent to  manufacturers *

## Reference replacement

| Reference extract | Sent to manufacturers | Manufacturers responding | Date shipped |
| --- | --- | --- | --- |
| E4 cat hair | 4/2/01 | 7 | 7/23/01 |
| C10 cat | 8/27/01 | 5 | 11/12/01 |

## Publications

*Patterson ML, Slater JE. Characterization and comparison of commercially available German and American cockroach allergen vaccines. Clin Exp Allergy (in press)*
*Sutherland MF, Drew A, Rolland JM****, ****Slater JE, Suphioglu C, O'Hehir RE.  Specific monoclonal antibodies and human IgE show Hev b 5 is an abundant allergen in high protein powdered latex gloves. Clin Exp Allergy (in press)*
* *

## Publications (reviews)

*Morrow KS, Slater JE. Regulatory aspects of allergen vaccines in the US. Clin Rev Allergy Immunol 2001; 21:141-152.** *
*Lockey RF, Slater JE, Esch R.  Preparation and standardization of allergen vaccines. In Middleton’s Allergy: Principles and Practice, 6**th** ed. St. Louis: Mosby (in press).*

## Abstracts

*Patterson ML, Cardinale EJ, O’Hehir RE, Sutherland MF, Slater JE. Hev b 5 double mutant proteins. J Allergy Clin Immunol 2002. *
*Valerio C, Solanki MD, Slater JE. The effects of intratracheal lipopolysaccharide (LPS) on the cytokine responses of Balb/C mice to ovalbumin. J Allergy Clin Immunol 2002. *
*Alston M, Valerio C, Morrow K, Dixon A, Slater JE. Lyophilization does not affect the activity or secondary structure of cat allergen vaccines. J Allergy Clin Immunol  2002.  *
*Soldatova LN, Marcovic-Housley Z, Mueller UR, Slater JE. The role of glycosylation in the IgE binding of the major honeybee allergen, hyaluronidase. J Allergy Clin Immunol 2002. *
*Finlay WJJ, Slater JE. Analysis of IgE heavy chain sequences from a small volume of peripheral blood from a mildly atopic individual. J Allergy Clin Immunol 2002.  *
*Hsieh LS, Moharram RM, Akasawa A, Slater J, Martin BM, Banana allergen detection and identification using SELDI technology. J Allergy Clin Immunol 2002.*
*Rabin RL, Alston, M, Cooley, J, and Farber, JM. Dual roles for the chemokine receptor CXCR3 in adaptive immunity. **J Allergy Clin Immunol 2002.*