# Presentation: 679126

## BER Personnel Changes

- _NEW HIRES_
- Bob Vallario- Integrated Assessment Program 	Manager
- Elizabeth White- Human Subjects Protection Officer
- _PENDING_
- Associate Director-BER
- Division Director- Environmental Remediation
- Computational Biologist-Life Sciences
- Microbiologist/Geneticist- Climate Change

**Notes:**
NOTES:
FY 2006
We got the Presidents Request
Conference Direction:
GTL - Conference Report states: “The conferees support the development of the proposed Genomes to Life (GTL) facilities, and encourage the Department to budget for the first of these GTL facilities, for the production and characterization of proteins and molecular tags, in fiscal year 2007. The conferees encourage the Department to reduce the cost of the GTL facilities to accelerate deployment of all four proposed GTL centers. Due to the nature of this research, there is a need for all of the facilities to be deployed to meet the scientific challenge of molecular characterization. The conferees recommend that the Department conduct an open competition for the siting of these GTL facilities.” 

EMSL - $3.5 million in Congressional direction for additional equipment upgrades for the Environmental and Molecular Sciences Laboratory at the Pacific Northwest National Laboratory is “Within available funds,...”.

What’s Out
- All the molecular nuclear medicine and radiopharmaceutical research programs at the universities and national labs will be eliminated (with the exception of BNL).
- Elimination of all surficial science including radioecology and surficial fate and transport research in Environmental Remediation will lead to a nearly complete loss of funding for the Savannah River Ecology Laboratory.  This reduction has already resulted in numerous layoffs of SREL scientific staff.

GTL - $87.2

## BER Funding Profile(dollars in millions)

- 								     	**FY 2006****	****	****   FY 2007****	****	****      FY 2008         **
**	****	****	****	****	****	****	****	****      **_**Appropriation**_**      **_**Appropriation**_**            **_**President’s Request**_**	**
**Biological & Environmental Research****	****	****	**
**	****Life Sciences****	****	****	****	****	****	****197.7****	****	****	****     ****233.6****	****	****	****282.3****	**
**	****Climate Change Research****	****	****	****	****136.6****	****	****	****     134.4****	****	****	****138.1**
**	****Environmental Remediation****	****	****	****  91.2****	****	****	****       93.8****	****	****	****  97.4**
**	****Medical Applications & Measurement **
**	****	****Science****	****	****	****	****	****	**_**  13.5**_**                          **_**21.7**_**  ****	****	****	****  **_**14.0**_
**Subtotal, BER****	****	****	****	****	****	****439.0****	****                        483.5****	****	****	****531.9**
**Congressional Direction****	****	****	****        **_** 125.1**_**                            **_**0.0**_**                ****	**_**    0.0**_
**Total, BER****	****	****	****	****	****	****         564.1****	****	****              483.5****	****	****	****531.9**

**Notes:**
NOTES:
FY 2006
We got the Presidents Request
Conference Direction:
GTL - Conference Report states: “The conferees support the development of the proposed Genomes to Life (GTL) facilities, and encourage the Department to budget for the first of these GTL facilities, for the production and characterization of proteins and molecular tags, in fiscal year 2007. The conferees encourage the Department to reduce the cost of the GTL facilities to accelerate deployment of all four proposed GTL centers. Due to the nature of this research, there is a need for all of the facilities to be deployed to meet the scientific challenge of molecular characterization. The conferees recommend that the Department conduct an open competition for the siting of these GTL facilities.” 

EMSL - $3.5 million in Congressional direction for additional equipment upgrades for the Environmental and Molecular Sciences Laboratory at the Pacific Northwest National Laboratory is “Within available funds,...”.

What’s Out
- All the molecular nuclear medicine and radiopharmaceutical research programs at the universities and national labs will be eliminated (with the exception of BNL).
- Elimination of all surficial science including radioecology and surficial fate and transport research in Environmental Remediation will lead to a nearly complete loss of funding for the Savannah River Ecology Laboratory.  This reduction has already resulted in numerous layoffs of SREL scientific staff.

GTL - $87.2

## BER Highlights

- New Laboratory Funding Plan
- Climate Change Research
- -Committee of Visitors Review, 5/25-27
- -Division Strategic Plan

-    Environmental Remediation
- -Awards: Lawrence, R&D 100

**Notes:**
NOTES:
FY 2006
We got the Presidents Request
Conference Direction:
GTL - Conference Report states: “The conferees support the development of the proposed Genomes to Life (GTL) facilities, and encourage the Department to budget for the first of these GTL facilities, for the production and characterization of proteins and molecular tags, in fiscal year 2007. The conferees encourage the Department to reduce the cost of the GTL facilities to accelerate deployment of all four proposed GTL centers. Due to the nature of this research, there is a need for all of the facilities to be deployed to meet the scientific challenge of molecular characterization. The conferees recommend that the Department conduct an open competition for the siting of these GTL facilities.” 

EMSL - $3.5 million in Congressional direction for additional equipment upgrades for the Environmental and Molecular Sciences Laboratory at the Pacific Northwest National Laboratory is “Within available funds,...”.

What’s Out
- All the molecular nuclear medicine and radiopharmaceutical research programs at the universities and national labs will be eliminated (with the exception of BNL).
- Elimination of all surficial science including radioecology and surficial fate and transport research in Environmental Remediation will lead to a nearly complete loss of funding for the Savannah River Ecology Laboratory.  This reduction has already resulted in numerous layoffs of SREL scientific staff.

GTL - $87.2

## BER Highlights

-    Life and Medical Sciences
- -GTL Bioenergy Centers Selection
- -NAS Report on “State of the Science in Nuclear     Medicine”, July 2007
- -JGI Update

**Notes:**
NOTES:
FY 2006
We got the Presidents Request
Conference Direction:
GTL - Conference Report states: “The conferees support the development of the proposed Genomes to Life (GTL) facilities, and encourage the Department to budget for the first of these GTL facilities, for the production and characterization of proteins and molecular tags, in fiscal year 2007. The conferees encourage the Department to reduce the cost of the GTL facilities to accelerate deployment of all four proposed GTL centers. Due to the nature of this research, there is a need for all of the facilities to be deployed to meet the scientific challenge of molecular characterization. The conferees recommend that the Department conduct an open competition for the siting of these GTL facilities.” 

EMSL - $3.5 million in Congressional direction for additional equipment upgrades for the Environmental and Molecular Sciences Laboratory at the Pacific Northwest National Laboratory is “Within available funds,...”.

What’s Out
- All the molecular nuclear medicine and radiopharmaceutical research programs at the universities and national labs will be eliminated (with the exception of BNL).
- Elimination of all surficial science including radioecology and surficial fate and transport research in Environmental Remediation will lead to a nearly complete loss of funding for the Savannah River Ecology Laboratory.  This reduction has already resulted in numerous layoffs of SREL scientific staff.

GTL - $87.2

## JGI’s Contribution to WW Sequencing

## JGI User Community Growth

## Sequencing Production at the DOE Joint Genome Institute (2007)

**0.57 ¢**

-  **0.19****¢**

**0.35****¢**

**Sequence production**** (Billions of bases/month)**

**0.50**

**  ****1.00**

**0**

**Cost: Cents per base**

**1.0**

**0**

**2.0**

**3.6**

**1989**

**1991**

**1993**

**1995**

**1997**

**1999**

**2003**

**2005**

**2001**

**0.46****¢**

-  **0.10****¢**

**2007**

-  **0.09****¢**

## DOE Bioenergy-Relevant Plants

**“****Feedstocks”**

***Populus trichocarpa***** **
**(black cottonwood) **

**Switchgrass**** **

**Soybean**** **

***Sorghum***

**Cassava  **
**(*****Manihot esculenta*****)**** **

***Brachypodium ***
***distachyon***** **

***Zea Mays (Corn)***** **

## DOE Bioenergy Relevant Microbes (source of enzymes)

***Acidothermus cellulolyticus***** ATCC 43068**

***Clostridium thermocellum***

***Cytophaga hutchinsonii***** ATCC33406**

***Frankia***** Cc13**

***Pichia stipitis***

***Saccharophagus degradans***** 2-40 **(formerly *Microbulbifer degradans*)

***Thermobifida fusca***** YX**

***Moorella thermoacetica***** ATCC39073**

***Nostoc punctiforme***

***Rhodopseudomonas palustri***

## Next Generation Sequencing Technologies (Sequencing by Synthesis)

**454 Life Sciences**
**30-40 MB/run**

**Solexa**
**200-1,000 MB/run**