# Presentation: 502887

## NSLS-IILife Science Breakout Session

## Agenda

-  Introduction (Miller)
-  Keynote Speaker: Carolyn Larabell (ALS, UCSF) (30 min)
-  Technique talks (4 min each)
-  Imaging
-  Soft x-ray spectromicroscopy (Jacobsen)
-  Diffraction Imaging (Jacobsen)
-  Full-field transmission x-ray microscopy (Larabell)
-  X-ray fluorescence micro/nanoprobe (Vogt)
-  Diffraction-enhanced imaging (Zhong)
-  Microbeam radiation therapy (Dilmanian)
-  Infrared microspectroscopy and imaging (Miller)
-  Structural & Molecular Biology:
-  X-ray footprinting (Chance)
-  XANES/EXAFS (Chance)
-   Macromolecular Crystallography (Stojanoff)
-  Small-Angle X-Ray Scattering (Yang)
-  Discussion
-  Closeout (Sweet)

## Life Sciences Discussion Session Summary

- What are the _key scientific drivers_? What experiments will NSLS-II enable that are not presently possible?
- What _technical capabilities_ will these require? (Beamlines, endstations, undulators...)
- Estimate of _community size_.
- What _detector_ requirements does this field have? Do these require R+D?
- What _software and computing_ infrastructure requirements are there? (Control, data acquisition, analysis)
- Any particular _accelerator_ requirements?
- Any particular _conventional facility_ requirements?

## Cross-Technique Imaging

-  infrared imaging: chemical imaging of biological cells
-  full-field, soft x-ray TXM: nanoscale 3D sub-structure
-  x-ray spectromicroscopy: 3D nanoscale chemical imaging of organic components
-  x-ray fluorescence microprobe: 3D trace element mapping

**Cross-Technique Imaging**

**Cross-Technique Structural/Molecular Biology**

- For the same protein:
-  small-angle x-ray scattering: low-resolution structure, static & dynamic
-  EXAFS: metal active site environment, static & dynamic
-  MX: atomic-resolution structure, static & dynamic

**Key Scientific Drivers**

**Life Sciences “Village” Environment**

## Technical Capabilities 

-  Imaging
-  Soft x-ray spectromicroscopy (ID for tomography)
-  Diffraction Imaging (ID)
-  Full-field transmission x-ray microscopy (BM)
-  X-ray fluorescence micro/nanoprobe (ID)
-  Diffraction-enhanced imaging (SCW)
-  Microbeam radiation therapy (ID)
-  Infrared microspectroscopy and imaging (2 BM)
-  Structural & Molecular Biology:
-  X-ray footprinting (3PW)
-  XANES/EXAFS (DW)
-   Macromolecular Crystallography (6 IDs)
-  Small-Angle X-Ray Scattering (1 ID, 1 3PW)

## Community Size

**Community Size**

**0**

**500**

**1000**

**1500**

**2000**

**2500**

**3000**

**1990**

**1991**

**1992**

**1993**

**1994**

**1995**

**1996**

**1997**

**1998**

**1999**

**2000**

**2001**

**2002**

**2003**

**2004**

**2005**

**2006**

**Fiscal Year**

**# Users**

- Chemical Sciences

- Materials Sciences

- Life Sciences

- Environmental and Geoscience

- Applied Science and Engineering

- Optical/Nuclear/General Physics

- Unspecified

**45% in 2006**

## Community Size

- 65% macromolecular crystallography
- 30% other structural/molecular biology (spectroscopy, SAXS, footprinting)
- 5% imaging

- TOTAL

- Life Sciences

**Community Size**

- *FY06

**Notes:**
About 65% of life science pubs are PX; 30% other structural/molecular biology (spectroscopy, SAXS, footprinting); 5% imaging

## Quality of Life 			

- Offices for beamline staff NEAR beamline; lab space near beamline
- Sufficient floor space at beamline, AND office like controls environment

- Laboratories and ancillary facilities
- Wet lab, with basic equipment (e.g., BSL2, cell culture, crystal growth, protein purif.)
- Light, epifluorescence, confocal microscopes
- Sample prep, in particular freezing, microtoming (ultra, cryo, RT)
- Access to correlated (same mounts ?) electron, IR, and soft x-ray microscopies

- Adequate staffing !!!!!!
- Including people with significant background in biological sample prep.
- Only supporting beamline ops is insufficient, need to support whole experiment (planning, sample prep, data analysis, interpretation)!

**Life Sciences “Village” Environment**

- Accelerator
- Meet specs

- Detectors
- Limiting today for: MX, EXAFS, IR

- IT
- Handling of very large datasets
- ‘full’ remote control of beamline, remote access; cybersecurity, firewall issues