# Presentation: 405608

## Start-up Procedureand Clean-up Procedure

## When you walk in the system should have..

- Dust cover on the microscope
- Objectives out of the way and lowered all the way
- All the objectives cleaned
- Long working distance condenser for DIC images on the microscope
- Olympus microscope turned off (switch located at the right rear of the scope)
- Mercury lamp turned off
- Instrument control PC and power strip (orange toggle switch) turned  turned off  in the Electronics cabinet

## To start instrument controller

- Log onto the Linux machine and start the SoftWorx program
- Turn the power strip on (orange switch located on the lower right on the electronics cabinet)
- Turn the microscope on (switch located rear right of scope)
- Turn the instrument controller PC on (marked in the cabinet)
- Initialize stage:
- Toggle to PC screen
- Make sure the objectives are lowered and out of the way
- Make sure micro-motion stage is empty
- Follow instruction on screen to initialize the stage
- Before leaving the PC screen, make sure that the controller window is highlighted
- Toggle to Linux screen

## Toggle between PC and Linux box

- Marked on the keyboard are two keys (2x and PC)
- To toggle between screens:
- Press the key below 2x twice then the key above the PC once
- You should see the 3 lights above the number pad flashing
- The screen will switch between the screens
- To get access to the keyboard again hit ESC, the 3 lights should stop flashing

## To start the mercury lamp

- If using the mercury lamp turn the power supply to the lamp (located on the desk top, Photometrics box).
-  Log date, time and reading on the box at start and end of session as well as the user name in the book to record hours on the lamp.
- Allow the mercury lamp to warm up for 1⁄2 hour.
- Once the lamp has been turned off, do not turn it back on for at least 2 hours. If someone else is using the microscope after you do not turn the lamp off.

## Koehler Illumination and bright field

- Koehler illumination:
- Put desired objective into place, oil if necessary
- Put cover slip on the stage, Make sure there is something you will be able to see by bright field on it. Noise oil is good
- Open the condensor into the bright field position (“Pol” on the eyepiece setting)
- Bring the condensor down (to about 2cm above the sample)
- Open the transmitter light shutter. You should see bright light through the eyepiece
- To focus, look at the coverslip directly (not through the eyepieces), bring the objective up until the oil touches. Then look through the eyepieces and continue bringing the objective up until your sample is in focus.

## Koehler Illumination and bright field

- Close the field diaphragm all the way
- Use the condensor focus know on the edge of the field diaphragm (looking through the eyepieces). Do not use the objective focusing knobs for this step
- Center the field diaphragm with the 2 centering screws
- Open the field diaphragm until it just fills the field of view  (for camera it will be closed even more than for visual work because the camera field is narrower
- Bright Field:
- First step up Koehler illumination
- Looking through the eyepieces adjust the condensor until the image is the best compromise of contrast and resolution.

## Clean-up Procedure for oil objectives

- Clean outside well of the objective using lens paper
- Do not touch the part of the lens paper that you will use.
- Clean the well around the objective
- To clean the surface of the objective
- Dip a cotton swab into the chloroform and shake of excess into trash can.
- In a rolling motion wipe the surface of the objective with the cotton swab. Dispose of the swab, do not reuse, repeat until the objective is clean and not streaky.
- To check for streaks, take the removable eyepiece and use it as a magnifying lens to look at the surface of the objective

## Shut-down for instrument controller

- Toggle to the PC screen
- While cursor is on the DV controller window, hit Esc twice. This will shut down the instrument controller and close the window
- Shut down PC normally using the Start Menu
- Toggle to Linux screen
- Turn off power strip (orange toggle switch)  in the Electronics cabinet
*when leaving the room, please leave the microscope in the configuration described in “when you walk in the system should have..”*