# Presentation: 552201

## WEST NILE VIRUS SURVEILLANCE & CONTROL

**WEST NILE VIRUS SURVEILLANCE & CONTROL**
**ACTIVITIES IN EAST BATON ROUGE PARISH, LA**
**GUY FAGET, FRED AUGUSTINE, ALEX FOLSOM, DAN GARRETT,**
**HERFF JONES, RANDY VAETH, ROD WELLS, & MATTHEW YATES**
**     ****EAST BATON ROUGE MOSQUITO ABATEMENT & RODENT CONTROL**

## Typical Arbovirus Transmission Cycle

**Typical Arbovirus Transmission Cycle**

## Dead-end Host

**Dead-end Host**

**Dead-end Host**

## Phases of Arboviral Transmission

**Jan-Mar    Maintenance Phase**
**Apr-June    Amplification Phase**
**July-Aug    Early Transmission**
**Oct-Dec     Late Transmission**

## SURVEILLANCE

## Birds

## Northern Cardinal

- Blue Jay

- House Sparrow

**WNV Bionomics-East Baton Rouge Parish Birds of Interest**

## Wild Bird Blood Samples

**Wild Bird Blood Samples**

## Sentinel Chickens

## Notable Bird Species + for WNV 2004

**Notable Bird Species + for WNV 2004**
**                     ****TOTAL = 101**

## Notable Bird Species RT-PCR+ for WNV 2005

**Notable Bird Species RT-PCR+ for WNV 2005**
**                         ****TOTAL = 34**

## WNV+ SENTINELS 2004, 2005

**WNV+ SENTINELS 2004, 2005**

## % OF ALL WNV+ BIRDS 

**N. CARDINALS = 57%**
**HOUSE SPARROWS = 21%**

**% OF ALL WNV+ BIRDS **
**MAY-NOV 2004, 2005**

## OTHER WNV+ BIRDS = 22%

**                 ****(MAY-NOV 2004, 2005)**
**BLUE JAY**
**N. MOCKINGBIRD**
**BROWN THRASHER**
**COMMON GRACKLE**
**MOURNING DOVE**

##  Mosquito Surveillance

** ****Mosquito Surveillance**

## Quink breeding in proximity 

**Quink breeding in proximity **
**to houses**

## Tire pile in proximity to houses

**Tire pile in proximity to houses**

## High density Quink breeding

**High density Quink breeding**

## Modular Wastewater Treatment Plants

**Modular Wastewater Treatment Plants**

## Breeding & Resting Sites

**Breeding & Resting Sites**

## Storm Drain Surveillance

**Storm Drain Surveillance**

## Adult Mosquito Trapping Techniques

**Adult Mosquito Trapping Techniques**

**Rotator Trap**

**New Jersey Trap**

**Modified Gravid Trap**

##       Southern House Mosquito

**      ****Southern House Mosquito**
**(up to 70% positive in July, August)**

## Asian Tiger Mosquito

- Aedes vexans

- Culex nigripalpus

- Culex restuans

**Other potential**
**vectors**

## Cx. salinarius

**Cx. erraticus**
**Cx. coronator**
**An. crucians**
**An. quadrimaculatus**
**Ps. ferox**
**Ps. howardii**
**Ae. canadensis**
**Ae. fulvus pallens**

## Slide 26

## 1st human cases = MLE ~6-8/1000

**1****st**** human cases = MLE ~6-8/1000**
**Late season cases possibly from hunting activity**

## 1ST human case = MLE of 6/1000

**1****ST**** human case = MLE of 6/1000**
**Late season cases possibly from hunting activity**

##    CONTROL STRATEGY

**INTEGRATED MOSQUITO MANAGEMENT**

## Concentrated Larviciding in High Risk Areas

**Concentrated Larviciding in High Risk Areas**

## LARVAL CONTROL

**LARVAL CONTROL**
**Ditches: Vectolex WDG**
**95% control 7-10 days**

## Floodwater swales-Golden Bear Oil

**Floodwater swales-Golden Bear Oil**
**95-100% control**

## Modular Wastewater Treatment Facilities: 

**Modular Wastewater Treatment Facilities: **
**Altosid Pellets, Vectolex WDG, Arosurf**
**90-100% control up to 14 days**

## Tirepiles: Vectobac G

**Tirepiles: Vectobac G**
**100% control, early instar larvae**

## Mosquito minnows

**Mosquito minnows**

## Public Education

**Public Education**

## Aerial and ground adulticiding initiated during April

**Aerial and ground adulticiding initiated during April**
**& May, continuing through September, especially in **
**high risk areas**

**Ground ULV with pyrethroids**

**Aerial application with naled**

## Southern House Mosquito

**Southern House Mosquito**
**          ****A Hard Target**

**Asynchronous life cycle**
**2. Cryptic habitats, both resting and breeding**
**3. Active **_**ALL**_** **_**night**_** long**
**4. Ability to become tolerant to insecticides**

##                                          Summary of Results 

**                                      ****Cage Test 2004-3**
- Date 4-27-04
**Insecticide: Scourge 1:10**
- App Rate: 0.0013 lb/ac AI
- Species: Cpq
- Equipment: Leco
**Distance (ft)                   1 hr % Mortality             12 hr % Mortality**

| 100 |      
           98.7 |           100 |
| --- | --- | --- |
|  |          |  |
| 200 |           98.4         |          98.4 |
|  |  |  |
| 300 |    
          97.1      |          94.1  |
|  |  |  |
| 400 |           98.1 |          90.7 |
|  |  |  |
| 500 |          66.7   |          64.1 |
|  |  |  |
| UNTREATED |  
          0.0 |           0.0 |

## AERIALLY APPLIED DIBROM VS QUINKS 

**DISTANCE(FT)**

**% MORT.**

**AERIALLY APPLIED DIBROM VS QUINKS **

## DATE

**GRAVID TRAP #’****S**** FOLLOWING AERIAL APPLICATION 8/16/05**

**DATE**

**# QUINKS**

**.**

## Slide 42

## Bottle Bioassay Results

**August 2005 Scourge vs Quinks @ Keokuk**

**Bottle Bioassay Results**

## Slide 44

## CONCLUSIONS

- Human case onsets seem to begin when MLE’s reach ~6-8 +quinks/1000.
- Northern Cardinals (and to a smaller degree, House Sparrows) seem to play an important role.
- Integrated Mosquito Mgt techniques can reduce human/WNV interactions.

## East Baton Rouge Parish

**East Baton Rouge Parish**
**Surveillance Questions**

## What happens during weeks~26 – 34

**   ****when mosquito sample + percentages**
**   ****reach a maximum?**

## 2. How important are other vectors such

**    ****as Ae. albopictus?**

**222 pools submitted, 9 positive(4.05%)**

## 3.What happens during Wintertime

**3.What happens during Wintertime**
**   ****(maintenance phase)?**

## Slide 50

## PONDERING THE REALLY TOUGH DILEMMAS

**PONDERING THE REALLY TOUGH DILEMMAS**
**                   ****(LOUISIANA STYLE)**