# Presentation: 126582

## Mir ‘Free’ Surface Condensate

**Mir ‘Free’ Surface Condensate**

**“****SLIMY” SAMPLE**

**from BEHIND PANEL IN**

**KVANT II MODULE**

**1-15-98/NASA 6**

**“****SLIMY” SAMPLE**

**5-28-98/NASA 7**

**“****FRESH” SAMPLE**

**from  KVANT II ELEKTRON**

**5-28-98/NASA 7**

*Alcaligenes eutrophus*

*Alcaligenes faecalis*

*Bacillus coagulans*

*Alcaligenes latus*

*Bacillus circulans*

*Bacillus licheniformis*

*Citrobacter freundii*

*Bacillus coagulans*

*Bacillus pumilus*

*Corynebacterium aquaticum*

*Bacillus licheniformis*

*Bacillus*

- species

*Corynebacterium jeikeium*

*Bacillus pumilus*

*Comamonas acidovorans*

*Enterobacter agglomerans*

*Bacillus*

- species

*Corynebacterium*

- species

*Escherichia coli*

- CDC Group IVC 2

*Enterobacter cloacae*

*Hydrogenophaga flava*

*Citrobacter brackii*

- Presumptive

*Legionella*

- species

*Kingella denitrificans*

*Citrobacter freundii*

*Pseudomonas *

- species

*Methylobacterium*

- species

*Comamonas acidovorans*

*Rhodococcus*

- species

*Pseudomonas vesicularis*

*Corynebacterium*

- species

*Serratia liquefaciens*

*Serratia liquefaciens*

*Flavobacterium meningosepticum*

*Serratia marcscens*

- Unidentified gram negative rod

- Presumptive

*Legionella*

- species

*Sphingobacterium thalpophilum*

*Xanthomonas maltophilia*

*Pseudomonas fluorescens*

- Unidentified gram negative rods

*Serratia liquefaciens*

*Yersinia frederiksenii*

*Serratia marcescens*

*Yersinia intermedia*

- Unidentified gram negative rods

*Yersinia frederiksenii*

*Yersinia intermedia*

## Mir ‘Free’ Surface Condensate

**Mir ‘Free’ Surface Condensate**

**“****SLIMY” SAMPLE**

**from BEHIND PANEL IN**

**KVANT II MODULE**

**COLLECTED:  1-15-98/NASA 6**

- “SLIMY” **SAMPLE**

**COLLECTED:  5-28-98/NASA 7**

**“****FRESH” SAMPLE**

**from  KVANT II ELEKTRON**

**COLLECTED:  5-28-98/NASA 7**

- VISUAL

- OBSERVATION:

- Cloudy, brown particulate matter

- present

- Cloudy, brown particulate matter

- present

- Slightly cloudy, white particulate

- matter present

- WET MOUNT:

- Many bacteria and yeast; amoeba

- observed

- Many bacteria and yeast; few

- filamentous fungi; few ciliated

- protozoa; rare dust mite

- Many bacteria and yeast; few

- ciliated protozoa; rare dust mite

- SEM:

- Rod and spirochete form bacteria

- observed

- Rod and spirochete form bacteria

- observed

- Rod and spirochete form bacteria

- observed

- PARASITOLOGY:

- Ciliates resembling:

*    **Stylonychia *

- species observed

- Amoeba resembling:

*Acanthamoeba *

- species or

*Hartmanella*

- species observed

- No Parasites Observed

- No Parasites Observed

-            ANAEROBIC

- BACTERIA:

- Not Done

- None Isolated

- None Isolated

*MYCOBACTERIUM:*

- Not Done

- None Isolated

- None Isolated

- FUNGI:

*Acremonium*

- species

*Candida guilliermondii*

*Candida guilliermondii*

*Candida guilliermondii*

*Candida lipolytica*

*Candida lipolytica*

*Candida krusei*

*Cladosporium *

- species

*Fusarium*

- species

*Cladosporium *

- species

*Fusarium species*

*Hansenula anomala*

*Fusarium*

- species

*Hansenula anomala*

*Penicillium*

- species

*Penicillium*

- species

*Penicillium*

- species

*Rhodotorula glutinis*

*Rhodotorula rubra*

*Rhodotorula glutinis*

*Rhodotorula rubra*

*Rhodotorula rubra*

## Acceptability Limits

**Acceptability Limits**

**Parameter**

**NASA**

**Standards**

**RSA**

**Standards**

**Air**

**Total Bacteria:**

**1000 CFU/m**

**3**

**500 CFU/m**

**3**

**Total Fungi:**

**(inclusive in 1000)**

**100 CFU/m**

**3**

**Surfaces**

**Total Bacteria:**

**4000 CFU/100 cm**

**2**

**1000 CFU/100 cm**

**2**

**Total Fungi:**

**400 CFU/100 cm**

**2**

**100 CFU/100 cm**

**2**

**Water**

**Total Count:**

**100 CFU/100 ml**

**10,000 CFU/100 ml**

**Total Coliforms:**

**0 CFU/100 ml**

**Total Viruses:**

**0 PFU/100 ml**

**Food**

**Total aerobic count:**

**20,000 CFU/g**

**1,000 CFU/g**

**Escherichia coli:**

**1 CFU/g**

**1 CFU/g**

**Coag. + Staphylococci:**

**1 CFU/5g**

**1 CFU/g**

**Salmonella:**

**1 CFU/25g**

**1 CFU/25g**

**Clostridium perfringens:**

**100 CFU/g**

**100 CFU/g**

**Yeasts and Molds:**

**100 CFU/g**

**50 CFU/g**

## PCR Patterns of Staphylococcus aureus Isolates Found on Mir Missions

**Before Launch**

**After Return**

**4 – 5**

**months**

**2 – 3**

**months**

**1**

**month**

**0 – 14**

**days**

**Mission A**

**Crewmember #1**

**A***

**A**

**Crewmember #2**

**A**

**Crewmember #3**

**A**

**A,B**

**A,B,D**

**A,C**

**Crewmember #5**

**B,E,F,H**

**B,E,F**

**G**

**Crewmember #6**

**B***

**Environmental**

**Sample**

**A**

**Mission B**

**Crewmember #1**

**I**

**J,K**

**Crewmember #2**

**F,L**

**F,L**

**PCR Patterns of *****Staphylococcus aureus***** Isolates Found on Mir Missions**

*** Single isolates**

## Microbial Transfer on the Space Shuttle