# Presentation: 067001

## Laboratory of Molecular Technology (LMT)

**Laboratory of Molecular Technology (LMT)**

**915 Tollhouse Road, Frederick, MD**

- _[http://web.ncifcrf.gov/rtp/labs/LMT/](http://web.ncifcrf.gov/rtp/labs/LMT/)_** **

## Complete genomics facility

**Complete genomics facility**
**Technology platforms:**
**Genomic analysis**
**Molecular interactions**
**Gene expression:**
**Transcription**
**Post-transcription**
**Translation**
**Post translation**

## Access and develop new technologies

**Access and develop new technologies**
**Availability to NCI researchers**
**Core services**
**Collaborations**
**Apply to independent research programs**
**Ovarian development**
**Ovarian cancer**
**Prostate cancer**

## LMT Core Services:

**LMT Core Services:**

**Sequencing**
**‘****standard’**
**high-throughput**
**Microarray**
**Genotyping**
**Custom automation**
**Oligonucleotide synthesis**
**Molecular diagnostics**

## LMT Sequencing Core:

**LMT Sequencing Core:**

**4 ABI 377**
**slab-gel sequencers**

**3 ABI 3700**
**capillary sequencers**

**Sequencing in tube, 96-well, or 384-well formats**
**Data analysis**

**Current output: ~25,000 sequences / month (~95% of theoretical capacity)**

## GEOSPIZA INC.Software for Molecular Biology

**GEOSPIZA ****INC**.Software for Molecular Biology

## LMT Microarray Core:

**LMT Microarray Core:**

**2 BioRobotics MicroGrid II**
**microarray spotting robots**

**2 Axon GenePix 4000A**
**microarray scanners**

**~ 400 Incyte GEM II mouse arrays distributed / month**
**‘****other’ libraries**
**assistance with study design**
**assistance with data analysis**
**Affymetrix Workstation**

## LMT Molecular Diagnostics Core:

**LMT Molecular Diagnostics Core:**

**CLIA certified**
**Viral detection**
**DNA quantitation**
**Mouse speciation**
**Mycoplasma detection**
**Mutation detection**

## LMT Oligonucleotide Synthesis Core:

**LMT Oligonucleotide Synthesis Core:**

**Instrumentation:**
**2 ABI 394 oligonucleotide synthesizers**
**1 ABI Ezpedite oligonucleotide synthesizer**
**1 HP 1015 HPLC**
**Services offered:**
**‘****standard’ oligonucleotides**
**‘****modified’ oligonucleotides**
**PNA oligonucleotides**
**HPLC purification**
**Gel-purification**
**Output: ~3500 oligonucleotides/month**

## Genotyping

**Other LMT Core Services:**

**Genotyping**
**coming soon (July 2002)**
**Custom automation**
**automated colony picking**
**re-array**
**high density filter arrays (colony and DNA)**
**automated process development**
**custom automation software development**

## Slide 11

## LMT Research and Development:

**LMT Research and Development:**

**Currently ongoing R&D at LMT (independent and collaborative):**
**expression profiling:**
**ovarian metastasis **
**mouse ovarian development**
**rat ovarian development**
**mouse model of ovarian cancer**
**p27 and prostate tumor regression**
**prostate tumor prognostics and ethnicity**
**caveolin 1 function during colon cancer development**
**immune response**

## LMT Research and Development:

**LMT Research and Development:**

**Currently ongoing R&D at LMT (independent and collaborative):**
**microarray-based monoclonal antibody screening and selection**
**sera microarrays**
**DNA methylation / gene expression (NCI 60 cell-line panel)**
**electronic microarray development (Nanogen Corporation):**
**high-throughput monoclonal antibody production**
**multiplex analysis**
**electronic stringency**
**electronic addressing**
**universal detection**
**primer ‘picking’ software**

## LMT Research and Development:

**LMT Research and Development:**

**Currently ongoing R&D at LMT (independent and collaborative):**
**functional SNP association and prostate tumor prognostics/ethnicity**
**automated molecular screening**
**high-throughput LOH analysis**
**automated process development (Beckman Coulter)**
**automated, high-throughput ABI 7900 genotyping**
**universal detection system for ABI 7900 genotyping**
**virtual 2-D gel software**
**‘****real-time’ PCR primer picking software**
**ABI 7900 genotyping primer picking software**
**haplotype generation software**

## LMT Research and Development:

**LMT Research and Development:**

**R&D at LMT (in development or planned):**
**whole genome yeast 2-hybrid system**
**DNA methylation changes during mouse ovarian development**
**prostate tumor gene expression:LOH events**

## LMT Group Leaders:

**LMT Group Leaders:**
**Microarray-Lisa Gangi**
**Genomics-Shirley Tsang**
**Diagnostics-Kris Pike**
**Oligonucleotide synthesis- Leo Lee**
**Sequencing and Automation-Lynn Rasmussen**
**High-throughput-Claudia Stewart**
**Standard-John Elser**
**Automation-Casey Frankenberger**

**Collaborators:**
**NCI**
**J. Weinstein**
**M. Nickerson**
**R. Bedigian**
**J. Sharp**
**J. Coffin**
**S. O’Brien**
**M. Dean**
**B. Gold**
**J. Slattery**
**Johns Hopkins Medical Center**
**A. Feinberg**
**University of Chile**
**J. Allende**
**A. Quest**
**C. Cortes**
**LSU Medical Center**
**W. Rayford**
**University of Massachusetts**
**A. Jacobson**
**MIT**
**Sean Milton**