# Presentation: 765744

## OAT RUST RESEARCH at the PLANT BREEDING INSTITUTE

- PROF. ROBERT F. PARK
- ADJ. ASSOC. PROF. COLIN WELLINGS
-   DR. HAYDAR KARAOGLU
-   DR. DAVINDER SINGH
-  ADRIAN CABRAL

## Team members                                               

- _Prof. Robert F. Park_ (GRDC Chair-Cereal Rust Research), Director of the Australian Cereal Rust Control Program
- _Adj. Assoc. Prof. Colin Wellings_ (NSW DPI -seconded to Univ. of Sydney)
- _Dr. Davinder Singh_ (Postdoctoral Fellow, oats, wheat and barley)

## Research Areas                                               

**pathogen variability**
- pathogenicity surveys (Prof Park and Dr Wellings)
- genetic variation (with Dr. Karaoglu)
**genetics of resistance to crown rust**
- seedling (Australian oat cultivars + OCR differentials)
- APR : more than 50 accessions (6x) identified, 7 selected are being mapped in SSD populations
**genetics of resistance to stem rust**
- seedling (several sources of effective resistance being characterised)
- APR (several sources being characterised)

## Research Areas cont’d

**pre breeding, APR to crown rust:**
- single backcross approach-
- Portage/MN 841837//Riel
- Portage/MN 841837//Nobby
- seed will be made available to breeders
- proof of concept (can the single backcross approach, used to accumulate minor genes for rust resistance in wheat, be successfully applied to oats?)

**APR Donor 1**

**APR Donor 2**

**2-300 BCF****1**

**F****1**

**Adapted**
**Parent**

**Bulk Recurrent Selection for **
**Agronomic type and Rust resistance**

**X**

## Genetic variability in oat rusts

- _Dr. Haydar Karaoglu_ (Molecular biologist)
**Long term Aim**: develop SSR markers for 5 pathogenic species of *Puccinia i.e., Pgt, Pt,* *Pst, Pca *and* Ph.*
**Current work**: developing markers for the stem rust* P. graminis *(*Pgt*) and if applicable for *Pgs *(rye),* Pga, Pgl *(rye grass),* Pgt x Pgs* (Scabrum rust) *Pst *and* Psh* (Barley grass yellow rust)
**Progress to date**:
-  total of 11,731 SSRs (Di to hexanucleotides) identified from *Pgt* genome
- developed 20 polymorphic SSR markers for *Pgt*
- Ten SSRs (3%) amplify OCR - polymorphism to be tested
**Methodology**:
**-**	Identify/download most abundant SSRs from the sequenced *Pgt* genome using Python find; develop SSR markers using Oligo 6 software.
**-**	Apply the developed markers for pathogen variability of all Australian isolates

## Adrian Cabral – crown rust 

- 2nd Year PhD. Candidate
- Thesis: Genetics of Host: pathogen interaction in wild and cultivated *Avena: P coronata* f. sp. a*venae.*
- SSR variability studies in 2x and 4x wild oat accessions
- genetic analysis of resistance to *Pca *in 2x and 4x accessions
- multipathotype tests of 2x, 4x & 6x accessions to characterise resistance to *Pca* and *Pga*
- APR to *Pca* in selected accessions
- mapping studies

## Overall ..
- No SSR polymorphisms has been detected among Australian oat cultivars

Current - Future 
- Development of SSR markers for Australian wild oats
- Develop linked markers-Mapping of APR genes to Pc genes
- Seedling OCR markers to be identified/procured

Long term objectives
- Tease out OCR seedling resistance genes-create single gene stocks & map
- Utilise DArT 

- - No SSR polymorphisms has been detected among Australian oat cultivars
- _Current - Future__ _
**-** Development of SSR markers for Australian wild oats
- - Develop linked markers-Mapping of APR genes to *Pc* genes
- - Seedling OCR markers to be identified/procured
- _Long term objectives_
**-** Tease out OCR seedling resistance genes-create single gene stocks & map
- - Utilise DArT

- Adrian Cabral – crown rust