# Presentation: 660651

## FSIS E. coli O157:H7Verification Sampling9 CFR 417.8(g)New Project Codes

## FSIS E. coli 0157:H7

- _Purpose__: _Outline current FSIS verification sampling of raw beef products for *E. coli* O157:H7 in official establishments
- Several FSIS Notices changed policy provided in FSIS Directive 10,010.1.

## FSIS E. coli 0157:H7

- MT43 (Dir 10,010.1, FSIS Notice 86-07)
- MT44 (FSIS Dir 10,210.1 – formerly MT04)
- MT43S (FSIS Notice 12-08)
- MT50 (FSIS Notices 18-07, 62-07, 66-07, 68-07)
- MT52 (FSIS Notice 66-07)
- MT53 (FSIS Notices 17-07, 66-07)
- MT54 (FSIS Notice 68-07)

## FSIS E. coli 0157:H7

- _Policy Changes__ to Dir 10,010.1_:
- Raw Ground or Comminuted Beef Product *Routine *Sample projects:
- MT43 Risk-based Sampling (*formerly* MT03)
- MT43S added for *Salmonella sp. *
-       Low Volume Producers (< 1000 lbs/day)
*Not implemented until March 28th (Monday)*

## FSIS E. coli 0157:H7

- _Policy changes __to Dir 10,010.1_:
- Raw Ground Beef Component or Raw Beef Patty
*Routine *Sample projects:
- MT50 Raw Beef Manufacturing Trimmings used to make raw ground beef products - domestic
- MT54 Raw Ground Beef Components (including raw beef patty components) other than beef manufacturing trimming

## FSIS E. coli 0157:H7

- _Policy changes __to Dir 10,010.1_:
- Raw Ground Beef Sample projects:
- IPP do _not _to wait until the establishment completes pre-shipment review before submitting raw beef samples to the laboratory for *E. coli *O157:H7 testing.

## FSIS E. coli 0157:H7

- _Policy changes__ to Dir 10,010.1_:
- Raw Ground Beef Sample projects:
- IPP may submit the raw beef sample to the laboratory after the establishment has completed all interventions, except for any intervention that is based on microbiological test results.

## FSIS E. coli 0157:H7

- _Policy __*not*__ changed__ in Dir 10,010.1_:
- IPP may submit the sample either in
* *final packaged form prior to freezing (preferred method *unless* CCP for freezing)
- if held overnight before shipment then the final package is frozen or...

## FSIS E. coli 0157:H7

- _Policy __*not*__ changed__ in Dir 10,010.1_:
- IPP may submit the sample either in
- aseptic collection in a *sterile* whirl pak bag from the FSIS Field Services Laboratory
*Note: use of anything other than a sterile bag will result in a discard from the laboratory.*

## FSIS E. coli 0157:H7

- _Policy changes__ to Dir 10,010.1_: When positive “+” FSIS sample occurs...
- IPP should routinely collect follow-up samples as soon as possible following the FSIS positive result
- IPP do not wait for an establishment to complete corrective actions

## FSIS E. coli 0157:H7

- _Policy changes __to Dir 10,010.1_:
- Multiple follow-up FSIS samples after a “+” MT43, MT50, or MT05 (Retail)
- 16 or 8 samples (less than 1,000 lbs/day)
- (max 2/shift from different lots;
-                             minimum 3/week)

## FSIS E. coli 0157:H7

- _Policy changes__ to Dir 10,010.1_:
- Follow-up Raw Ground Beef
-         Sample project codes:
- MT44 (formerly know as MT04) multiple follow-ups after “+” MT43
- MT04T trace back to originating grinding establishment when “+” found at retail MT05

## FSIS E. coli 0157:H7

- _Policy changes__ to Dir 10,010.1_: Follow-up Raw Ground Beef Component or Raw Beef Patty Sample projects:
- Multiple samples for follow-up to the FSIS routine verification sampling “+” at establishment that grind beef *or* at retail store. (≥ 2 lbs)
- MT52 Follow-up Raw Beef Manufacturing Trimmings “+” – Originating slaughter establishment sampled

## FSIS E. coli 0157:H7

- _Multiple Follow-up Sample Verification_
- MT52 multiple follow-up samples
- Recommendation by OPPD RAD/OFDER DAIG
- 120 day review of STEPS
- If originating slaughter establishment only supplier
- If multiple originating establishments identified in STEPS

## FSIS E. coli 0157:H7

- _Multiple Follow-up Sample Verification_
- MT53 multiple follow-up samples after a “+” MT50 or “+” MT54 at slaughter establishment

## FSIS E. coli 0157:H7