# Presentation: 253724

## DeltaVisionTM 3.9 Upgrade Tutorial

## If you are viewing this presentation...

- Congratulations on your new ***DeltaVision*** Restoration Microscopy System Upgrade!
- We will now outline the features, benefits, and changes that were made.

## Overview

- Hardware
- Excitation Module
- Fiber Optic Cable
- Fiber Optic Module and Photosensor Redesign
- Emission Module
- Joystick/Keypad
- Electronic Cabinet Rack Mount
- Flat Panel Monitor
- Reporting Eyepiece Filterwheel
- Repeatable Slide Holder
- Optional upgrade to CoolSNAP HQ Fast Acquisition CCD camera

## Overview

- Software
- LINUX based acquisition and analysis workstation
- User-friendly Graphical User Interface
- Increased capabilities for acquiring 2D snapshots
- Stage cartoon with major improvements in navigation and point marking
- Ability to mark “top” and “bottom” for z-sectioning
- Filter Monitor window
- Printing capabilities
- OTF Library
- Overall improvements in software functionality

## Hardware

- Most of the new hardware was designed for enhancements in functionality, image quality,  and acquisition speed.

- Things that are significantly different from a user standpoint include:
- Fiber Optic Module / Photosensor
- Reporting Eyepiece Filterwheel
- Repeatable Slide Holder
- Joystick/Keypad

## Hardware – Fiber Optic Module/ Photosensor

- Critical/ Kohler illumination quick change
- Integrated fiber optic photosensor
- New, more flexible, higher efficiency fiber optic cable
- Compatibility with future FRAP option

- Critical Illumination Position

- Kohler Illumination Position

- Position Lock-down

## Hardware – Repeatable Slide Holder

- Repeatable Slide Holder allows users to replace slide in exact position so that point lists can be reloaded accurately
- Future inserts will be available for Nunc-type chamber slides and 35mm dishes

## Hardware – Joystick/Keypad

***Hardware – Joystick/Keypad***

- Joystick moves in X/Y only.
- Z is controlled by arrow keys.

- Point Mark

- Filter changing keys.

- Excitation Shutter

- Transmitted light Shutter

- Joystick speed controls

**Please take a moment to review the most common joystick/keypad functions.**

## Hardware -  Eyepiece Filterwheel

- Reporting Eyepiece Filterwheel improves usability with oculars.
- Manual change of EP filterwheel  electronically signals excitation and emission filterwheels to change to match.
- The Filter Monitor is large and color-coded on the user interface.

- ***Please note ND filter nomenclature has been changed – the percentage refers to the amount of light that passes through the filter.

## Software

- To eliminate confusion between different revisions, we will now review the main menus in Resolve 3D.
- While the main softWoRx toolbar has not changed, there have been performance improvements.

## Software - LINUX

- The Linux footprint is similar to the Start menu on Windows.  Go here to find Settings, Programs, Utilities, etc.

- File navigation is similar to Windows or Macintosh

- SoftWoRx can be started from either of the 2 icons or from the foot>Programs

## Software – Resolve 3D Filters & Lenses

- Filter and lens selection, image size, ‘Info.,” and binning have remained virtually unchanged.

- Improved ‘Find’ button enables user-defined maximum intensity.

- Acquire button has moved.  This will still acquire a single, temporary image in Window 21.

- Experiment launches experiment window which launches experiment designer.

- Settings launches tabular-style window interface  for camera settings, default file storage, and Window 21 properties.

## Software – Resolve 3D Drop-down Menus

- Many options are now hidden under drop-down menus.  Please take a moment to familiarize yourself with the new locations.

## Software – Resolve 3D Stage diagram

- Center object will move the stage to center over a point that you click on Window 21

- Marks a point

- 1, 2, 3, 4, 5, 6

- 1 - Marks top of sample
- 2 - Visits top of sample
- 3 - Marks bottom of sample
- 4 - Visits bottom of sample
- 5 - Visits middle of marked top and bottom
- 6 - The camera icon will acquire an image in Window 21

- X, Y, and Z stage movement functions have not changed.

- Shows user-defined sample thickness and relative location in Z

- Moves field of view in stage diagram

- Clears stage trails

- Opens point list window

- Stage diagram shows a cartoon of points that are marked and the stage’s path of travel

## Software – Resolve 3D Right-click

***Software – Resolve 3D Right-click***

- Right-clicking the mouse anywhere on the Resolve3D window will give you a menu with some common functions such as Acquire and Snapshot.  **Snapshot** is a great tool for acquiring and saving 2D multiple or single color images.

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## Software – Experiment Set-up

- The Experiment button in Resolve 3D will open the main Experiment interface.
- Image file name – the name of the image you are about to collect
- Image title – a description of the image written into the header
- Add note to log – adds text in the log file at the time you click the associated Do It; useful when adding a reagent during time-lapse
- Do It -  begins experiment

- ***NOTE:  Macros designed on an SGI-based DeltaVision will need to be recreated on your current machine.

## Software – Experiment Designer

- The Experiment Designer is launched from the Design/Run Experiment window.  It is very similar to the previous designer with a few enhancements.
- Get thickness – computes total z-thickness from marked top and bottom and fills in the # of sections automatically.
- Refresh exposure conditions – updates exposure times with information from Resolve3D; the wavelengths must already be set up for this button to function
- Fast acquisition...

## Software – Fast Acquisition

- Fast Acquisition was designed for the “fast” or interline CCD cameras.  These cameras are designed to do electronic shuttering where information is read off the chip while the next image is being acquired.
- The Fast Acquisition menu allows you to control the order and speed at which your experiment is collected.

## Software – OTF Library

- For your convenience, we have provided an OTF Library with optimal OTFs for standard ***DeltaVision*** lenses.
- Located in /usr/local/otf
- The OTF Library was created to give conservative consistent deconvolutions.
- A high quality OTF is better than a matched but poor quality OTF
- You may still choose to measure your own PSFs; Applied Precision is simply providing a baseline
- Confocal OTFs for several common objective lenses are supplied for deconvolving images acquired on confocal microscopy systems.
- Confocal OTFs are designed for typical confocal imaging conditions

## Additional Resources

- Additional help can be found under “Help” in any of the softWoRx windows or in your softWoRx 3.2 Manual.
- If you need assistance, please contact our customer help desk at 1.800.862.5166 or servicehotline@api.com.