# Presentation: 649832

## Consortium for Functional GlycomicsPamela A. Marino, Program Director

- 11/15/01

## Consortium for Functional Glycomics

**http://www.functionalglycomics.org**

** *****Define paradigms by which protein-carbohydrate interactions mediate cell communication***

**NIGMS sponsored 'glue grant' (~$8 MM/yr) most $ in cores. **
**Over 250 participating investigators worldwide**
**Focus in innate and acquired immunity**

- 11/15/01

## Consortium for Functional Glycomics

**Consortium for Functional Glycomics**

**Consortium Databases**
**MIT **

**Steering Committee**

- 11/15/01

## Structure of the Alliance

**NCI Tumor**
**Glycome**
**Laboratories**

**Coordination**
**Unit**

**NIGMS – CFG**
- _Cores_
- Databases
- Analytical Chemistry
- Synthetic Chemistry
- GlycoT Gene Arrays
- Glycan Arrays

**NCI – EDRN**
- _Components_
- DMCC

- 11/15/01

## Consortium for Functional Glycomics : Glycobiology Resources Offered 

**Consortium for Functional Glycomics : Glycobiology Resources Offered **

**Analysis of your RNA samples on a highly annotated custom gene microarray with 1800 human and mouse genes**

**Glyco-gene microarry**

**Glycan specificity of your carbohydrate binding protein on an array with 300 unique glycans representing glycoprotein and glycolipid glycans**

**Glycan array screening**

**Over 100 carbohydrate compounds represented on the glycan array are available with azido or biotin functional groups**

**Carbohydrate compounds**

**30 mouse strains with genes ablated for glycosyltransferase and glycan-binding proteins**

**Mouse knockout strains**

- _[http://](http://www.functionalglycomics.org/)__[www.functionalglycomics.org](http://www.functionalglycomics.org/)_

## Analytical Glycomics Core

**Human Glycomics: MALDI profiling of N- and O-glycans of tissues, primary cells.**
**Murine (and human) glycan profile data disseminated on website.**

## Glycan Array Core

**Printed Glycan array expanded to 300 glycans.**
**Perform Analysis of glycan binding proteins.**
**Results updated regularly on website.**
**.**

**SNA**

## Focused Glycogene Microarray 

**GLYCOv3 chip with up to date gene list highly annotated by participating investigators**
**Genes for: Glycosyltransferases; Glycan-binding proteins,Glycan degradation proteins; Notch pathway proteins; Nucleotide sugar synthesis and transporter proteins; N-glycan biosynthesis-related proteins; Adhesion molecules; Interluekins and receptors; Mucins; Growth factors and receptors; Cytokines; Chemokines; Conserved oligomeric Golgi (COG) complex proteins**
** **
**Data entered into database and posted on website.**

## Mouse Transgenics / Phenotyping Cores

**Over 30 KO lines available**
**Phenotypic data on website**

## CFG DATABASES

**Glycan Binding Protein Database: Molecule pages for 150 glycan-binding proteins **
**Glycan Structural Database: Over 7500 entries for *****N*****-linked, *****O*****-linked and synthetic glycans contain structural and chemical information, as well as PubMed links to the source articles.**
**Glycosyltransferase Database: Glycosylation pathways pages for different glycoconjugate classes. Arrows between structures link to the appropriate CAZY database page for the GT.  **
**power point demo of data bases here: http://www.functionalglycomics.org/static/consortium/CFG_Bioinformatics_Progress_2006.ppt**