# Presentation: 280178

## Application of Lifetime Analyses in the Upper Ocean to the Interpretation of Satellite-Based, Solar Induced Chlorophyll Fluorescence Signals Maxim Y. Gorbunov and Paul G. Falkowski 

**Abstract**
- The distribution of phytoplankton biomass and primary production in the ocean can be studied from satellite platforms (MODIS and MERIS) using solar induced fluorescence (SIF) from chlorophyll a (ca. 675 nm band). Although the SIF algorithm is potentially most advantageous in Case 2 waters, where DOM and suspended sediments interfere with chlorophyll algorithms based on blue-green water leaving radiances, the application of SIF to understanding either phytoplankton physiology or carbon fixation on a global scale has not been achieved. Chlorophyll fluorescence from phytoplankton is strongly affected by physiological state and taxonomic composition. Basic photosynthesis research over the last decade tremendously improved our understanding of the physiological mechanisms that control the quantum yields of chlorophyll fluorescence, however, the contributions of these mechanisms to the observed variability of SIF in the ocean remain largely unknown. Although the variability in SIF yields is correlated with environmental forcing, the mechanisms responsible for this variability are not known due to very limited field studies of related processes. In this start-up project, we develop an instrumental package to directly measure fluorescence lifetimes and yields in the ocean and to study the physiological mechanisms of variability in the quantum yield of SIF with an overarching goal to develop improved models and algorithms for retrieving phytoplankton biomass, physiological status, and the rates of primary production. The quantum yield of chlorophyll fluorescence is directly derived from Time Correlated Single Photon Counting measurements of fluorescence lifetimes and is integrated with the FIRe variable fluorescence technique. We envision that this research project will provide the scientific background for the interpretation of solar induced fluorescence signals in the ocean, help to improve MODIS biomass algorithms, and provide crucial physiological information needed for better estimates of primary production from remote sensing of the ocean color.

**Application of Lifetime Analyses in the Upper Ocean to the Interpretation of Satellite-Based, Solar Induced Chlorophyll Fluorescence Signals **Maxim Y. Gorbunov and Paul G. Falkowski

- Institute of Marine & Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08901

- This work is funded by NASA (NNX08AC24G, 2008-2010).

**Background and Problem Statement**
-   Solar Induced Fluorescence (SIF) from chlorophyll a (Chl-a) is detected as a red peak (ca. 675 nm) in spectra of water-leaving radiance.
-   SIF is the only signal emitted from the ocean and detectable from space that can be unambiguously ascribed to phytoplankton.
-   The SIF algorithm is potentially most advantageous in Case 2 waters, where DOM and suspended sediments interfere with chlorophyll algorithms based on blue-green ratios (Gower et al, 1999; Hoge et al, 2003; Siegel et al, 2005; Cullen, 2007; Huot et al, 2007).
-    While the huge variability (ca. 10x) in SIF yield in the ocean is often correlated with environmental forcing (Letelier et al, 1996; Morrison 2003; Huot et al, 2005), the mechanisms and interpretation of this relationship remain to be elucidated.

**Variable Fluorescence Measurements:**
** ****Assessment of Biomass and Physiological Status of Phytoplankton**

**F**luorescence **I**nduction and **Re**laxation (FIRe) System

- Single Measurement
- 			   Time Series

**Development of Sea-Going Instrumental Package to Measure Fluorescence Yields, Lifetimes, and Photosynthetic Characteristics of Phytoplankton**

**The Irradiance Dependence of Chlorophyll Fluorescence Yields**
- Fo and Fm are the minimum (open reaction centers) and maximum (closed centers) fluorescence yields measured in darkness.  Fo’ and Fm’ are the minimum and maximum fluorescence yields measured in light-adapted cells.  F’ is the actual fluorescence yield measured under ambient light that corresponds to the yield of remotely sensed Solar Induced Fluorescence.  PQ and NPQ are photochemical quenching (i.e., phytosynthetic use) and non-photochemical quenching (thermal dissipation), respectively.  While fluorescence yields under low light are primarily controlled by the photosynthetic efficiency, **SIF yields (under high light) are controlled by the magnitude of non-photochemical quenching (NPQ)**.  The measurements were made in a marine diatom, *Pheoductylum tr.,* by using a FIRe fluorometer (Gorbunov and Falkowski 2005).* *

**Objectives**
- Develop a sea-going instrumental package designed to directly measure fluorescence lifetimes and yields in the ocean.  The package integrates Time Correlated Single Photon Counting measurements of fluorescence lifetimes with variable fluorescence technique;
- Establish the physiological and biophysical mechanisms of variability in the quantum yield of SIF in the ocean with an overarching goal to develop improved models and algorithms for retrieving phytoplankton biomass, physiological status, and the rates of primary production.

**Laboratory Studies**

**References**
- Cullen, J. J., A. M. Ciotti, R. F. Davis and P. J. Neale. (1997) In: Ocean Optics XIII, S.G. Ackleson and R. Frouin, eds. Proc. SPIE 2963: 272-277.
- Gorbunov MY, and Falkowski PG (2005) In: “Photosynthesis: Fundamental Aspects to Global Perspectives” (Eds: A. van der Est and D. Bruce), Allen Press, V.2, pp. 1029-1031.
- Gower, J.F.R., R. Doerffer, and G.A. Borstad. (1999) - Int. J. Remote Sens. 20: 1771–1786.
- Hoge FE, Lyon PE, Swift RN, Yungel JK, MR Abbott, RM Letelier, and WE Esaias (2003) - Applied Optics, 42 (15): 2767-2771.
- Huot Y, CA Brown, and JJ Cullen (2005) - Limnol. Oceanogr.: Methods v.3.
- Huot, Y., C. A. Brown, and J. J. Cullen. (2007) - Journal of Geophysical Research, Vol. 112, C06013, doi:10.1029/2006JC003794
- Letelier, R.M., and M.R. Abbott (1996) - Rem. Sensing Environ., 58, 215-223, 1996.
- Morrison JR (2003) – Limnol. Oceanogr., 48(2): 618-631.
- Siegel, D. A., S. Maritorena, N. B. Nelson, M. J. Behrenfeld, and C. R. McClain (2005) -  Geophys. Res. Lett., 32, L20605, doi:10.1029/2005GL024310.

**Taxonomic variability in the capacity of non-photo-chemical quenching (NPQ)**.  Brown algae (diatom and dinoflagellates) exhibit the strongest NPQ due to the efficient light-induced xanthophyll cycle.  In contrast, red algae and cyanobacteria did not evolve xanthophyll cycle mechanism and show relatively weak NPQ that is driven by energy-dependent quenching (qE) induced by the pH gradient across the thylakoid membranes.

**Effect of photoacclimation on the capacity of NPQ.  **
- The capacity of NPQ decreases dramatically with decrease in growth irradiance.  Growth irradiance: HL = 500, ML = 100, LL = 20 mole quanta m-2 s-1. Measurements are made in the diatom, *Dilytum br*.