# Presentation: 275174

## Inhibition of Poly (ADP-Ribose) Polymerase (PARP) by ABT-888 in Patients With Advanced Malignancies: Results of a Phase 0 Trial

**Shivaani Kummar, MD**
**National Cancer Institute**
**September 5, 2007**

## Objectives

_**Primary**_**:**
**Determine a non-toxic dose range at which ABT-888 inhibits PARP in tumor samples and in peripheral blood mononuclear cells (PBMCs) .  **
**Determine the pharmacokinetics of ABT-888.**
**Determine the time course of PARP inhibition in PBMCs by ABT-888.**
_**Secondary**_**:**
**Determine the safety of administering one dose of ABT-888.**

## Eligibility Criteria

**Participants with solid tumors must have advanced disease refractory to at least one line of standard therapy or for which no standard therapy is available. **
**Participants with CLL or lymphoma may be enrolled if they have disease for which standard therapy is currently not indicated or disease that has failed at least one line of standard therapy. **
**Any prior therapy must have been completed ****≥ 2 weeks prior to protocol enrollment.**
**Adequate organ function.**

## Study Schema

**ABT-888**

**Tumor biopsies planned: **
**Significant PARP inhibition in PBMCs from at least 1 of the 3 participants at a given dose level, **
**OR**
******    ****Plasma C****Max**** of 210 nM achieved in at least 1 participant**

**Notes:**
10 mg = 6 mg/m2 = Mouse 2 mg/kg
25 = 15 mg/m2 = Mouse 5 mg/kg
50 = 30 mg/m2 = Mouse 10 mg/kg
100 = 60 mg/m2 = Mouse 20 mg/kg
200 = 120 mg/m2 = Mouse 40 mg/kg

## Study Schema

**The level of PARP expression in both tumor and PBMCs was determined using an ELISA assay prior to proceeding with drug administration and further sampling.**
**The required minimum level of PAR expression was defined as 31 pg PAR per mL of PBMC extract (allows for demonstration of a 50% reduction in PARP activity) prior to proceeding with sampling for PD studies. **
**All participants received drug and had sampling for PK studies.**

## Dose Escalation

**3 patients at each dose level**

**The objective of dose escalation was to investigate a PD endpoint, i.e., inhibition of PARP activity,**** ****and **_**not**_** to determine the maximum tolerated dose (MTD).**
**Dose escalation continued with the goal to achieve significant PARP inhibition in tumor samples in 3 out of 3 participants at 2 dose levels.**

| Dose Level | Dose |
| --- | --- |
| Level 1 | 10 mg |
| Level 2 | 25 mg |
| Level 3 | 50 mg |
| Level 4 | 100 mg |
| Level 5 | 150 mg |

## Trial Statistics

**Endpoints are PARP inhibition in tumor tissue and in PBMCs. For either endpoint:**
**Significant PARP inhibition **_***for a dose level***_** is defined as 2-fold reduction in PAR level for at least 2 patients out of the 3 accrued**
**If there is 80% likelihood of 2-fold reduction in PAR level***** ***_***for the patients***_***, *****then***** *****there is 90% power to declare significant inhibition **_***for the dose level***_**, by the binomial distribution.**** **

## Trial Results (To Date)

**14 patients enrolled on study, 11 are evaluable**
**3 patients (10 mg); 3 patients (25 mg); 8 patients ****(50 mg- 3 NE: tumor biopsy negative for PAR levels at baseline (1), 1 pt withdrew prior to receiving drug due to personal reasons, 1 pt currently being evaluated)**
**Age (range): 49-74 years**
**Diagnoses: carcinoid (1), colorectal cancer (3), small cell lung cancer (1), low grade lymphomas (3), CTCL (3), adenocarcinoma of the external auditory canal (1), SCC head and neck (1), melanoma (1)**
**Patients monitored by serial bloodwork, EKGs, physical exams**** **

## Mean Plasma Concentration of ABT-888 Following a Single Oral Dose

**Mean Plasma Concentration of ABT-888 Following a Single Oral Dose**

## PAR Inhibition in PBMCs

**Cohort 1**

**Cohort 2**

**Cohort 3**

**Pt 10**

**Pt 2**

**Pt 3**

**Pt 4**

**Pt 6**

**Pt 7**

**Pt 8**

**Pt 1**

**PAR Inhibition in PBMCs**

**Pt 11**

## PAR Inhibition in Tumor Biopsies 3-6 Hours Post Dose

## PAR Inhibition in PBMC and Tumor Biopsies 24 Hours Post 50-mg Dose

**PAR levels in PBMC samples from Patient 12 (*****levels in Patient 13 below defined minimum for continued sampling*****)**

**PAR levels in tumor biopsy samples from Patients 12 and 13 **

## First Phase 0 - Timelines and What We Have Achieved

## First Phase 0 - What Have We Achieved?

**Established that ABT-888 inhibits the target of interest at clinically achievable concentrations.**
**Established target assay feasibility in human samples after qualification in animal models. Assay validated in preclinical models using clinical procedures.**
**Developed SOPs for human tissue acquisition, handling and processing.**
**Performed real-time PK and PD analyses (results received within 72 hours of obtaining sample).**
**PK and PD data, including timing of tumor and PBMC sampling, available well before planned Phase I combination studies.**

## The Next Speaker is:Dr. Steve Larson

**The Next Speaker is:****Dr. Steve Larson**