# Presentation: 691961

##  High Throughput BankingcaTissue Usage at Indiana University

**Presented By Amber McMahon**
**Drs. Susanne Ragg and Gunther Schadow**
***“******Tissue Banks & Pathology Tools” Face-to-Face***
***June 2008***

## Agenda and Goals

**	****	**_**Usage Focus Points**_
- Study Calendar
- How do we use it?
- Why do we use it?
- Barcoding
- How do we use it?
- Why do use it?

**	****	**_**Overview and Introduction**_** **
- Indiana University and Regenstrief Institute
- High throughput laboratory
- Have been involved since 2004

## Overview—Who comes into contact with caTissue through Indiana? 

**Departments (Indiana University)**
- Pediatric Hematology/Oncology
- Pathology
- Ophthalmology
- Cardiology
- Obstetrics/gynecology
**Institutions **
- Local (Monarch Life Sciences, IU, Riley Hospital)
- National (Children’s Oncology Group, Purdue University)

**We deal with a wide range of departments, collection protocols, and sample types**

## Overview—How does IU use caTissue? 

**Ophthalmology**
- Aqueous humor
- Vitreous fluid
- Plasma/Platelet Poor Plasma/Serum
**Osteosarcoma, Wilms, Neuroblastoma**
- Plasma/Platelet Poor Plasma/Serum
**Routine Surgical Pathology**
- Frozen tissue
**Ewings**
- Plasma/Platelet Poor Plasma/Serum
- Urine
**Non-Normal**
- Plasma/PNP/Serum

_**Protocols with varying collections**_

**Obstetrics/gynecology**
- Plasma/Serum
- Cord Blood
- Placental tissue
**Cardiology**
- Plasma/Serum
**Circulating endothelial Progenitors**
- Plasma/Platelet Poor Plasma/Serum
- Tumor
**Normal**
- Plasma/PNP/Serum
**Leukemia**
- Bone Marrow
- Cells
- CSF

## Study Calendar

- Translate time-points from COG trial into caTissue

**Goal: Close 100% specimen collected**

**Notes:**
Discuss week time points and selection of one of 4 different treatment arms after surgery.

## Study Calendar

**Why do we use the Study Calendar?**

**Lab Organization and Scheduling**

**Quality Control**

**Traceability **

**Workflow evaluation**

**Notes:**
Need screenshot of missing things to make point;  could show them features

## Study Calendar 

**Why do we use the Study Calendar?**
- Lab organization and scheduling
- To know when our next sample is due

**caTissue**

**Next sample**
**date for EVERY**
**participant**

- Query

**Can also incorporate queries into workflow **

**Notes:**
Need screenshot of missing things to make point;  could show them features

## Study Calendar 

- Technician is made aware that sample may be entered under incorrect treatment arm.

**Why do we use the Study Calendar?**
- Lab organization and scheduling
- Track treatment arm switches

**Notes:**
Need screenshot of missing things to make point;  could show them features

## Study Calendar 

**Study Calendar **

**Why do we use the Study Calendar?**
- Quality Control

- Example: Investigate why sample was not collected

**Query to report collection efficiency**

**Total Samples Collected**

**Total Samples Scheduled**

- =

**Low efficiency prompts us to review workflow**

- Can report efficiency for single or multiple protocols and/or patients

**Notes:**
Need screenshot of missing things to make point;  could show them features

## Study Calendar 

**How do we use the Study Calendar?**

- Set up protocol in caTissue

- Register participant to protocol

- Enter sample data and
- create aliquots

## Study Calendar 

- Translate protocol from lab to caTissue:
- Collect Whole Blood
- Centrifuge Whole Blood to create serum and plasma
- Aliquot serum into cryovials
- Centrifuge a portion of the plasma a second time to create platelet poor plasma
- Aliquot remaining portion of plasma
- Aliquot platelet poor plasma once created
- Freeze

- Serum

- Plasma

- Platelet poor plasma

- 1st Spin

- 1st Spin

- 2nd Spin

- Set up protocol in caTissue

## Study Calendar 

- Serum

- Plasma

- Platelet poor plasma

- 1st Spin

- 1st Spin

- 2nd Spin

- Set up protocol in caTissue

## Study Calendar 

- Register participant to protocol

## Study Calendar 

- Enter sample data and
- create aliquots

## Study Calendar Take Home Message

**Translates complicated treatment and laboratory protocols into a software environment that is easy to navigate**
**Allows for simple, quantitative evaluation of workflow efficiency**
**Increased consistency in a high throughput laboratory**

## Barcoding

**Goal: **Automated Data Capture
- Replace most tedious data entry tasks
- Records all relevant preparation events with time
- Multiple different workflows supported
- Keyboard free, just scan badges, forms, freezers, containers, vials, devices during specimen preparation and handling

- Aliquot data

- Blood draw data

- More aliquot data

- Event data (x3)

- Assign storage
- locations

- Currently    > 15mins

## Barcoding--Workflow 

**Sample kits made**
**w/ barcodes**

**Sample drawn &**
**Tubes scanned**

**Sample delivered**
**To lab**

**Tech scans **
**personal ID**

**Tech scans **
**sample**

**Sample aliquoted**
**Into vials **
**w/ barcodes**

**Tech scans **
**freezer box**

**Tech scans vial**

## Barcoding Take Home Message

**Saves on data entry time**
**Only data entry needed within caTissue is for editing purposes**
**Increased accuracy in a high throughput laboratory**

## General Conclusions 

**General Conclusions **

- Indiana University is a high throughput lab with diverse study protocols and sample collections.
- As such, we must place emphasis on workflow efficiency and resource management.
- Study calendars and barcoding are two tools that help us accomplish these goals.

## Thank you for your attention!

**QUESTIONS?**