# Presentation: 409751

## Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. 

- Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data.

**Blake A. Schaeffer**, D. Kamykowski,
- J. M. Morrison, S. Banks,
- A. McCulloch, and W. V. Sweet

## Slide 2

## Introduction

- The Galapagos harbors such great diversity because of its positioning in a complex transition zone between tropical, subtropical and upwelled waters.
- Only place in the world where hammerhead sharks, penguins, and sealions co-exist on the same sub-tidal reef.

## Introduction

- Three major current systems influence the Galapagos.
- Cool southern currents from the Humboldt (Peru) system influencing the southern islands.
- Warmer Panama currents that influence the northern islands.
- Strong, large-scale Equatorial Undercurrent (EUC) upwelling in the western archipelago.

## Focus

- The EUC is the major oceanographic feature affecting production in the Galápagos, and is strongly associated with local biogeography of marine species.
- Galápagos biogeography/marine ecosystem heterogeneity is a function of oceanographic patterning over small spatial, seasonal and inter-annual scales.

## Goals

- Hyperspectral information combined with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data will identify variability of phytoplankton biomass throughout the Galapagos.
- Hyperspectral information will elucidate phytoplankton taxonomy of the Galapagos with the support of HPLC pigment analysis, particulate absorption spectra and BreveBuster data.

## Methods

- Sierra Negra
- 70 hydrographic stations
- 12 days

## Methods

- Phytoplankton
- 20 m net
- 20 meters depth
- Filtration
- Surface only
- Extracted chlorophyll a
- Particulate absorption spectra
- Seapoint FL
- Top 2 meters bin average

## Methods

- MODIS
- Chlorophyll a
- SeaDAS v 4.9
- BreveBuster
*In situ* spectrometer
- Phytoplankton class spectral libraries
- Day and night travel
- HyperSAS
- 166-channel MiniSpec radiance and irradiance sensors
- Day travel only

## March 2005

- Chlorophyll a
- (g/L)

- Wet season
- Panama current
- dominates.

## November 2005

- Chlorophyll a
- (g/L)

**324**

**347**

- Transition
- from garua (dry) to wet season.
- Panama current replaces Peru current.

## June 2006
Chlorophyll a 
(g/L)

- Transition from wet to garua (dry).

- June 2006
- Chlorophyll a
- (g/L)

**190**

**181**

## MODIS chlorophyll a 

- MODIS chlorophyll *a*
- shows similar trends and range to Seapoint FL chlorophyll *a*.

- MODIS chlorophyll *a* show similar
- trends to extracted chlorophyll *a*,* *
- but less agreement in range.

- HyperSAS identifies variability not present in MODIS chlorophyll *a*.

## Remote sensing reflectance

## Particulate absorption

- Chlorophyll a

- Phycocyanin

- Chlorophyll a

- Carotenoids

## Particulate absorption spectra

## BreveBuster Phytoplankton Class ID

## BreveBuster Phytoplankton Class ID

## Phytoplankton Tows

## Future work

- HPLC pigment analysis
- Chemtax
- Particulate absorption spectral library
- Remote sensing reflectance 4th derivative analysis
- HyperSAS phytoplankton class spectral library
- _Poster_: 4.28-P    Today 12:00
- Seasonality of phytoplankton distributions in the Galapagos Marine Reserve

- Chlorophyll a?

- Carotenoids?

- Chlorophyll a?

## Thank you!

- Questions?