# Presentation: 847458

## Response to BERAC Review

**Overview of JGI**
**BERAC Recommendations**
**Managing multi-lab environment**
**Operation of user facility**
**LSP program**
**JGI Energy Portfolio**

**Notes:**
THANK YOU
NEW MEMBERS

## JGI Mission

***   “******User facility providing DNA sequencing & analysis in support of DOE missions in alternative energy, carbon cycling & bioremediation.”***

***www.jgi.doe.gov***

**Notes:**
Need 3 things to be DNA sequencing user facility: Sequencing, users, and science. 

## Cost-effective Sequencing at JGI

*NIH data provided by Jane Peterson, NHGRI*

- Cntr1

- Cntr4

- Cntr3

- Cntr2

- Cntr5

- Cntr6

***JGI***

**FY06 capacity: 30 billion basepairs**

**Notes:**
metrics

## Success of JGI User Programs

**Notes:**
The growth of our two primary user programs reflects an insatiable desire for DNA sequence.  The proposals submitted to the CSP in its recent cycle exceed the worlds’ sequencing capacity.  

## Primary JGI User Distribution   

**KEY:  ****RED=Academic****; ****Blue=National Laboratory****; Black=Industry****  **

**Notes:**
Over 600 individual users

## 1st Annual JGI User Meeting 

_**JGI User Meeting, April 2006**_
- 250 Users and potential users
- Sequence-based science talks
- Tours of JGI facility
- Poster sessions
- User Committee formed

**Notes:**
Great science
Users meet each other and JGI scientists and staff
User committee

## The JGI Partnership

- JGI Director

- PGF

- LBNL

- LANL

- ORNL

- SHGC

- PNNL

**The **_***J***_**GI Partnership**

## BERAC RecommendationsOperations and Management-1

**Management of LBL/LLNL workforce**
**Coordination of safety training & tracking**
***EH&S MOU complete, waiting signatures***
***LBL has 1o responsibility for EH&S***
**Cyber-security MOU**
***Committee configured, meets this month***

## BERAC RecommendationsOperations and Management-2

**Succession planning for key JGI positions**
***Hired production sequencing lead***
***Senior managers identified “seconds” ***
***Seconds contribute to weekly mgmt meetings***

## BERAC RecommendationsOperations and Management-3

**Coordination of JGI partner lab tasks:**
***Weekly genome management conference call***
***Monthly partners conference call***
***“******Finishing in the future” meeting***
***Eukaryotic Genome Program summit***

## BERAC RecommendationsUser Facility

- Proposals

- Sequencing

- Annotation

## BEERAC RecommendationsUser Facility-1

**Managing the User Facility:**** **
**Peer review**
***MGP and CSP programs/review groups merged***
***Broadened geographical representation***
***Electronic proposal submission & review***
***Letter-of-intent process***
***User committee input on peer review committees ***

## BEERAC RecommendationsUser Facility-2

**Managing the User Facility:**
**Formal project management**
***Appointed project management lead***
***Hired 2 new project managers***
***Launched Global Project Tracking System***
***End-of-project survey in draft form***

**Notes:**
David has passion for project management

## BERAC RecommendationsUser Facility-3

**Managing the User Facility:**
**Improve large genome analysis throughput**
***JAZZ assembly program overhauled***
***2 programmers hired into assembly group***
***3 people hired into annotation group***

## Lab Science Program

**LSP created by new JGI MOU **
***Facilitate access to JGI for Natl. Lab investigators***
***Fulfill programmatic sequencing needs of DOE***
***Shape JGI products and services to meet needs of the Natl. Labs ***
**BERAC concerns**
**Scientific vision for LSP**
**Implementation**

## BERAC RecommendationsLSP Program

**BERAC Recommendations***LSP Program*

**LSP lead**
***Appointed Jerry Tuskan, ORNL, Jan ‘06***
***Focus on bioenergy***
***LSP lead now reports to JGI Director***
**Implementation**
***Lab points-of-contact identified***
***Small-scale sequencing program***
***14 proposals received, 10 approved to date***
***Large-scale sequencing program***
***Plant Genomes for Energy white paper***
***Fungal Genomes for Energy white paper***

## Bioenergy & the JGI

**Bioenergy & the JGI**

- Corn based ethanol

- Soy-based biodiesel

**NSF/USDA/DOE**
**funded project**

**LSP project**

- Transportation Fuels

**Notes:**
Biomass has considerable potential as a replacement for petroleum in transportation fuels, and a 20% share of the transportation market is considered feasible by 2030.  However, ethanol currently accounts for just 2% of the fuel market and biodiesel a mere 0.01%.

## JGI and Bioenergy

- Fermentation

- Saccharification

**Sugars**

**Cellulose**

## Auxin Regulation of Poplar Shape

**Enhanced radial growth of  IAA16.3 transgenic trees**

**IAA16.3**

**Control**

**90-day-old *****Populus***** cuttings**

## New Cellulase Genes from Termite Gut

**Glc**

**H****2****O**

**22 ********-glucosidase**

**P**

**Glc**

**Glc-1-P**

**P**

**Cellobiose**
**phosphorylase**

**12 Exoglucanases**

**Cellulose**

**Cellobiose**

**15 Endoglucanases**

**New Cellulase Genes from Termite Gut**