**[Program of the:]{.underline}**

**32^nd^ Annual**

**Eastern Fish Health Workshop**

**18 -- 22 June, 2007**

**Eisenhower Inn and Conference Center**

**Gettysburg, Pennsylvania**

**National Fish Health Research Laboratory**

**USGS - Leetown Science Center**

**11649 Leetown Road**

**Kearneysville, West Virginia 25430**

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**[Monday , June 18]{.underline}**

5:00 -- 8:30 pm **Registration And Reception**

**Lee/McClellan/Grant Salons**

Hors d'oeuvres and Cash Bar

**8:00 Welcome to the 32nd Annual Eastern Fish Health Workshop**

**8:30 [Unusual Diagnostic Experiences]{.underline}, chaired by Andy
Noyes and Mark Matsche**

**Longstreet Salon**

8:30 Jones Esocid Lymphosarcoma In Lake Champlain 1

8:40 Bowser The Case Of A Worm In A Bottle 2

8:50 Lumsden 'Bloat' - The Most Abused Term In Fish Health? 3

9:00 Dove Not Your Average Patient: Whale Shark Medicine 101 4

9:10 Groman Unusual Myocardial Pathology Of Farm Reared Atlantic Halibut
5

> (*Hippoglossus hippoglossus*)

9:20 Wolf A Walk on the Wild Side: SelectiveHistopathologic Findings
From 6

> Endocrine Disruption Studies

9:30 Warg An Epizootic Of Cyprinid Herpesvirus 2 (Goldfish Herpesvirus)
In A 7

> Goldfish-Koi Production Farm

9:40 Walker Right Treatment; Wrong Results 8

9:50 Knight Pigmented Tumors In Gizzard Shad From The Black Warrior
River, Alabama 9

10:00 Durborow Online Fish Diseases Course At Kentucky State University
10

10:20 Bakal Current Status Of The U.S. National Aquatic Animal Health
Plan 11

10:40 **Adjourn**

**[Tuesday, June 19]{.underline}**

7:00 **Breakfast Lee/McClellan/Grant Salons**

*Assorted* *fruit, chilled juice, country scrambled eggs, bacon, fruit
crepes, pancakes with syrup, assorted biscuits and muffins, cold
cereals, coffee, tea, and milk.*

**[Proceedings]{.underline}** **Sheridan/Longstreet Salons**

**Emerging Disease Problems in Crustaceans, chaired by Jeff Shields**

8:00 Shields Comparison Of The Biology Of *Hematodinium* Infections In
Blue 12

> Crabs (*Callinectes sapidus*) And Snow Crabs (*Chionoecetes opilio*)

8:15 Ratzlaff Characterization Of *Panulirus argus* Virus 1 From The
Caribbean 13

> Spiny Lobster

8:30 Li Primary Culture Of Hemocytes From The Caribbean Spiny Lobster,

> *Panulirus argus* And Their Susceptibility To *Panulirus argus* Virus
> 1 (PaV1) 14

8:45 Messick Ailments In Captive Crabs 15

9:00 Kiryu Parasites And Diseases Of The Blue Crab *Callinectes sapidus*
From Tampa 16

> Bay, Florida

9:15 Schott Aspects Of The Molecular Biology Of *Hematodinium* sp. 17

9:30 Burge Shrimp Hemocyte Immunity: Peroxinectin Expression In Response
To 18

> *Vibrio* Infection

9:45 Chistoserdov The Role Of Microorganisms In Development Of Epizootic
Shell 19 Disease In The American Lobster

10:00 **Morning Break** *Coffee, tea*

**[General Session 1]{.underline} - Gretchen Messick, moderator**

10:30 Sharp Effects Of Lipopolysaccharide On Total Hemocyte Counts Of
The 20

> Blue Crab, *Callinectes sapidus*

10:45 McLaughlin An Overview Of Clam Health In The Chesapeake Bay 21

11:00 Xu Antimicrobial Peptides From The Suppression Subtractive
Hybridization 22

> cDNA Library Of Zebra Mussel (*Dreissena polymorpha*) Byssus Glands

11:15 Winters Identification Of Microbial Communities Associated With
The Zebra 23

> Mussel (*Dreissena polymorpha*)

11:30 Knight Health Evaluations Of Impinged Fishes At Steam Generating
Power Plants 24

11:45 Bebak Strategies For Field Sampling When Large Sample Sizes Are
Required 25

12:00 **Lunch Lee/McClellan/Grant Salons**

*Open faced turkey sandwich, green beans almandine, rolls, butter,
carrot cake, coffee, tea, and iced tea.*

**DNA, RNA, Protein Technologies: Not just for CSI; Fish can use them
too, chaired by Laura Brown**

1:00 Brown Genomics And Proteomics: What Do These Add To Fish Health
Research? 26

1:15 Rise Genomic Approaches For Studies Of Fish Responses To Pathogens
And 27

> Pollutants

1:30 Gomez-Chiarri Application Of Genomics To Study Host-Pathogen
Interactions In Oysters 28

1:45 Fast Real-Time Immunology: Analysis Of Gene Expression To Determine
29

> Fish Health

2:00 Nash DNA Microarrays To Study Bacterial Pathogenesis In Fish Health
Research 30

2:15 Dacaynay Metabolomics; Continuing Post-Genomics refinement 31

2:30 Robertson Expression of Antimicrobial Peptide Genes (Hepcidin 1 And
2) In 32

> Largemouth And Smallmouth Bass

2:45 Discussion

3:00 **Afternoon Break** *Coffee, tea, sodas*

**[General Session 2]{.underline} - Stephen Smith, moderator**

3:30 Russell Immunohistochemical Localization Of Two Putative Defense
Lectins, 33

> Rainbow Trout Ladderlectin And Intelectin In Diseased Rainbow Trout
> (*Oncorhynchus mykiss*) Tissue

3:45 Jaso-Friedmann Analysis Of A Novel And Phylogenetically Conserved
Pattern Recognition 34

> Receptor (PRR)

4:00 Connor A Novel Pattern Recognition Receptor On Nonspecific
Cytotoxic Cells Has 35

> Bimodal Functions Of Membrane Expression And Bactericidal Activity

4:15 Machen Immune Response Of Hybrid Striped Bass (*Morone saxatilis x
M. chrysops)* 36

> To A Commercial Vibrio Vaccine

4:30 Kane Girlie Boyz: Low-Level Endocrine Disruption In Fatheads 37

Salierno

4:45 Lupica Assessment Of Environmental Nitrate Stress Effects In Fishes
38

5:00 **Adjourn**

6:30 **Country Cookout Reception and Dinner with the 2^nd^ South
Carolina String Band**

**Lee/McClellan/Grant Rooms**

6:30 Reception with Cash Bar

7:30 Dinner

*Steamship round of beef, fried chicken, barbecued country pork ribs,
chili and crackers, baked potatoes, cole slaw, ambrosia, corn on the
cob, corn bread and biscuits, butter and honey, dessert table, coffee,
tea, and iced tea.*

**[Music of the 2^nd^ South Carolina]{.underline}**

**[Wednesday, June 20]{.underline}**

7:00 **Breakfast Lee/McClellan/Grant Salons**

*Assorted* *fruit, chilled juice, Western scrambled eggs, bacon, sausage
links, French toast with syrup,, assorted biscuits and muffins, cold
cereals, coffee, tea, and milk*

**[Proceedings]{.underline}** **Sheridan/Longstreet Salons**

**Diving Deeper: The pursuit of truth in aquarium medicine, chaired by
Charlie Innis**

8:00 Nyaoke Advances In Our Understanding Of Phaeohyphomycosis 39

8:15 Frasca Histopathologic Interpretation Of Lesions In Cephalopods 40

8:30 Weber Microbiology Testing For Aquatic Systems: Findings And
Potential 41

> Implications
>
> 8:45 Gargan Phagocytosis In Nurse Shark (*Ginglymostoma cirratum*)
> Peripheral Blood 42
>
> Leukocytes

9:00 Corwin Head And Lateral Line Erosion Syndrome (HLLES) In Ocean
Surgeonfish, 43

(*Acanthurus bahianus*), Current Efforts To Determine Etiologies

9:15 Hadfield Gas Supersaturation In A Large Artificial Reef Exhibit: An
Unusual 44

> Presentation And Resolution

9:30 LePage Retrospective Summary Of Diseases Affecting Captive
Seahorses At The 45

> Toronto Zoo

9:45 Poll Surgical Treatment Of Lymphoma In A California Moray Eel, 46

> *Gymnothorax mordax*

10:00 **Morning Break** *Coffee, tea*

**The cutting edge of cutting fish: surgery and anesthesia, chaired by
Greg Lewbart**

10:30 Lewbart Fish Sutures: Tying Things Together 47

10:45 Roberts To Cut Is To Cure: Surgical Cases In Fish Medicine 48

11:00 Bakal Endoscopic Sex Determination And Sterilization Of Gulf 49

> Sturgeon (*Acipenser oxyrinchus* Desotoi)

11:15 Innis Ophthalmic Surgical Procedures In Public Aquarium Fishes 50

11:30 Klide Anesthesia Of Fish: I Don\'t Want To Make The Wrong Mistake
51

> (Yogi Berra)

11:45 Clauss Comprehensive Wound Care Management In A Cownose Ray 52

> (*Rhinoptera bonasus)* And A Blue Catfish (*Ictalurus furcatus*)

12:00 **Lunch Lee/McClellan/Grant Rooms**

*Sautéed beef tips with blended rice, honey glazed carrots, sherbet,
coffee, tea, and iced tea.*

**[General Session 3]{.underline} - Renate Reimschuessel, moderator**

1:00 Quinn Comparison Of Lethal And Non-Lethal Sampling Techniques For
53

> The Detection Of *Aeromonas salmonicida* And Other Fish Pathogens
>
> In Populations Of Ontario Salmonids

1:15 Smith, S. Efficacy Of Common Disinfectant Against Aeromonas spp.
And 54

> Edwardsiella spp.

1:30 Wooster Florfenicol Uptake And Depletion In Tilapia (*Oreochromis
niloticus*) 55

Of Various Sizes

1:45 Darwish Minimum Inhibitory Concentration Testing Of *Flavobacterium
columnare* 56

2:00 Welch Sequence Analysis Of The *Yersinia ruckeri* Multidrug
Resistance 57

> Plasmid pYR1

2:15 Nolan Pharmacokinetics Of Oxytetracycline In The Horseshoe Crab 58

2:30 Yanong Use Of 17-alpha-methyltestosterone For Masculinization Of
Ornamental 59

> Fish

2:45 Kilgore Preliminary Investigations Into The Use Of Metomidate
Hydrochloride 60

> (Aquacalm^®^) In Ornamental Cichlids

3:00 **Afternoon Break** *Coffee, tea, sodas*

**[General Session 4]{.underline} - Al Camus, moderator**

3:30 Cassidy-Hanley Gene Discovery In *Ichthyophthirius multifiliis*: A
Complementary 61

> DNA Resource

3:45 Quinnell Cormorants As Mechanical Vectors Of *Heterosporis* sp. 62

4:00 Smolowitz Renal Trematodiasis Of Largemouth Bass And Development Of
PCR 63

> Methods

4:15 Dill Biology And Management Of A Monogenean Trematode
(*Acolpenteron* 64

> *ureterocetes*) Infecting Largemouth Bass (*Micropterus salmoides*)

4:30 Schulz Prevalence And Impact Of Leeches Parasitizing Fish Of Lake
St. Clair, 65

> Michigan

4:45 Discussion

5:00 **Adjourn**

**Dinner on your own**

5:30 **Gettysburg National Historic Battlefield Tour**

**[Thursday, June 21]{.underline}**

7:00 **Breakfast Lee/McClellan/Grant Salons**

*Assorted* *fruit, chilled juice, country scrambled eggs, bacon, sausage
links, pancakes with syrup,, assorted biscuits and muffins, cold
cereals, coffee, tea, and milk.*

**[Proceedings]{.underline}** **Sheridan/Longstreet Salons**

**Unraveling Complexities of Coral Pathogens and the Etiology of Coral
Diseases, chaired by Laurie Richardson**

8:00 Richardson Toxin Production And Virulence Factors In A
Polymicrobial 66

> Disease Of Corals

8:15 Gantar Antibacterial activity of cyanobacteria associated with
black band disease 67

8:30 Polson Comparison of Collection Methodology, Location, Coral
Species, and 68

> Health Status on Acroporid Coral Microbial Community Composition

8:45 Smith, G. Defense Mechanisms In Gorgonians Against Infections By
*Aspergillus* 69

> *sydowii*

9:00 Peters Histology Of White Plague-Diseased Corals 70

9:15 Cook Large-Scale Geographical Comparisons Of Bacterial Communities
71

> Associated With Corals Exhibiting Signs Consistent With White Plague
>
> Type II

9:30 Beauchamp Molecular Genetic Screening For Putative Antimicrobial
Genes In 72

> Anthozoans: A Possible First-Line Defense Against Coral Disease
> Pathogen

9:45 Discussion

10:00 **Morning Break** *Coffee, tea*

**[General Session 5]{.underline} -- Paul Bowser, moderator**

10:30 Heami Correlation Between API-ZYM And 16S rRNA-RFLP Profiles Of 73

> Ontario *Flavobacterium psychrophilum* Isolates

10:45 Yazdanpanah Cold-Inducible Proteins Of *Flavobacterium
psychrophilum,* The Cause 74

> Of Coldwater Disease

11:00 Good A Prospective Case-Control Study Of Bacterial Gill Disease
Outbreaks 75

> In Ontario, Canada Government Salmonid Hatcheries

11:15 Lowry Unique Mycobacterial Resistance And Clearance In Channel
Catfish 76

> (*Ictalurus punctatus)*

11:30 Williams DNA Fingerprinting of *Edwardsiella ictaluri* Using
Pulsed Field Gel 77

> Electrophoresis And Repetitive Sequence-PCR

11:45 Loch Pseudokidney Disease In Captive Salmonine Broodstocks 78

12:00 **Lunch Lee/McClellan/Grant Salons**

*Lasagna with garlic bread. Red velvet cake, coffee, tea, and iced tea.*

**[General Session 6]{.underline} - John Lumsden, moderator**

1:00 Yeh Molecular Cloning, Expression And Genome Organization Of
Channel 79

> Catfish (*Ictalurus punctatus*) Matrix Metalloproteinase-9

1:15 Al-Hussinee Viral Hemorrhagic Septicemia Virus (VHSV) Type IV′b′
Experimental 80

> Infection In Ontario Rainbow Trout

1:30 Groocock Viral Hemorrhagic Septicemia In New York State 81

1:45 Faisal Emergence And Spread Of VHSV In Michigan 82

2:00 Merrill Federal Regulatory Issues Regarding Viral Hemorrhagic
Septicemia (VHS) 83

2:15 Kibenge Molecular Correlates Of Infectious Salmon Anemia Virus
Virulence 84

2:30 Groman Diagnostic Methodologies For Detection of Beta-nodavirus
Infections 85

> Of Atlantic Cod (*Gadus morhua*)

2:45 Densmore Tadpole Edema Virus: Comparative Susceptibility Among
Early Life 86

> Stages of the Fowler's Toad (*Bufo fowleri*)

3:00 **Afternoon Break** *Coffee, tea, sodas*

**Putting It All Together: A Manager\'s Perspective On Aquatic Animal
Health, chaired by Frank Panek**

3:30 Panek Pathogens Associated With Native And Exotic Trout Populations
87

> In Shenandoah National Park And The Relationships To Fish
>
> Stocking Practices

3:45 Wiggins Perspectives for Trout Management and Fish Health in the
Batten Kill 88

4:00 Reeser CSI Shenandoah: The Importance Of Understanding Fish Health
In A 89

> Riverine Fish Kill Investigation

4:15 Jacobs Bio-Indicators Of Estuarine Health: A Multi-Variate Approach
Linking 90

> Source To Resource

4:30 Cipriano Evaluating Population Health Of Endangered Fishes By
Non-lethal 91

> Analysis Of Microbial Diversity

4:45 Discussion

5:00 **Adjourn**

6:30 **32nd Annual EFHW Reception and Banquet**

**Sheridan/Lee/McClellan/Grant Rooms**

6:30 Cocktail reception (cash Bar)

7:00 *Sliced roast beef with mushroom sauce, oven baked chicken, sliced
baked sugar cured ham, sliced roast turkey breast, pasta primavera,
tossed salad, fruit salad, pasta salad, vegetable medley, parsley
buttered potatoes, assorted pies, cakes and puddings, coffee, tea, and
iced tea*

8:00 **Presentation of the Best Student Paper Award**

**Banquet Dance (Cash Bar)**

11:30 **Adjourn**