Pathology case study

Laboratory Data Acquisitions System

  ----------------------------------- -----------------------------------
  **Document Number:**                TD-1002-2.0

  **Version:**                        2.0

  **Issued:**                         July 9, 1999

  **Reviewed & Approved by:**         John Minnich, Program Director

                                      

  **Date:**                           
  ----------------------------------- -----------------------------------

Pathology Case Study 05999/01

Training Study: Stardust 2010

National Toxicology Program Support

Pathology Case Study 05999/01

> Prepared for: National Institute of Environmental Health Sciences
>
> Research Triangle Park, NC 27709
>
> Contract no.: 273-95-I-0012
>
> Prepared by: Technology Planning & Management Corporation
>
> Imperial Center
>
> Canterbury Hall, Suite 310
>
> 4815 Emperor Boulevard
>
> Durham, NC 27703

Revision Summary

  -------------- -------------------- --------------- --------------------
  **Date         **Document Number**  **Revision      **Reason for
  Released**                          Level**         Revision**

  7/1999         TD-1002-2.0          2.0             Update for
                                                      implementation of
                                                      LDAS II.

  10/1994        TD-1002-1.0          1.0             New
  -------------- -------------------- --------------- --------------------

Table of Contents

Revision Summary

Table of Contents

Section 1 4

Introduction 4

Overview 5

Section 2 6

Logon 6

test selection 7

pathologist selection 7

Section 3 8

Assigning Protocol Required Tissues 8

Section 4 8

Defining Observations 8

Section 5 8

Building Observations 8

Introduction

This document contains step by step instructions and data sets necessary
to familiarize personnel with the Laboratory Data Acquisitions System II
(LDAS II) software.

the Laboratory Data Acquisitions System (LDAS) has been upgraded to an
NT platform using an object oriented Oracle database programmed in C++.
This upgrade accomplishes many things:

-   It makes LDAS Y2K compliant

-   It upgrades the equipment

-   Provides a faster and more robust system

-   Utilizes WWW to obtain and transfer reports

-   Adds mouse capabilities to the system

Although the initial release was designed to incorporate technological
advances into the original system, some enhancements have been added to
streamline the data collection process. This document provides the
necessary information to allow the end user to become familiar with the
enhancements by providing step by step instructions as well as
representative data sets that emulate actual study data. This case study
will also demonstrate many of the common error/warning messages that the
average end user could expect to see.

NOTE

> This case study contains instructions and data sets a subchronic Rat
> inhalation study. This data will be used to simulate Pathology data
> collection.

# Overview

This case study is organized into these five sections:

+--------------------------------------------------+-------------------+
| 1.  Logon and choosing a study                   |                   |
+--------------------------------------------------+-------------------+
| 2.  Assigning Protocol Required Tissue           |                   |
+--------------------------------------------------+-------------------+
| 3.  Define Observations                          |                   |
+--------------------------------------------------+-------------------+
| 4.  Building Observations                        |                   |
+--------------------------------------------------+-------------------+

During the data collection sessions, you will:

-   review animal removal data and treatment group notes

-   define protocol required tissues

-   define and use a personal dictionary of frequently used terms

-   enter tissue status

-   enter observations

-   enter tissue and animal notes

While performing pathology data collection, keep the following points in
mind:

-   The function keys ( in dark blue) at the bottom of the screen may
    change with the screen. Be sure to scan each screen for function key
    options.

-   You may access on-line HELP at any time by pressing the F1 key

-   You may use the mouse enhancement. A simple point and click!

If you need additional information on data entry, refer to the
*Pathology Workstation User's Guide.*

You are now ready to begin the section of this case study titled *"Logon
and Test Selection"*

Logon & Test Selection

During this session you will practice:

-   Logging on to the workstation

-   Selecting a Study

-   Reviewing pertinent Test information

-   Selecting a Pathologist

To complete this section, proceed through the following steps:

1.  Turn on the workstation. The logon screen should appear.

2.  At the **ENTER LOGON ID** prompt, type in your logon ID and press
    enter. The cursor moves to the **ENTER PASSWORD** prompt.

3.  Type in your password and press enter. The Set System Clock window
    should appear.

4.  At this point, you may confirm the time and date displayed by
    pressing enter twice, or you may alter the date and time pressing
    enter after each change. The Pathology Main Menu appears.

5.  You may access the Pathology Data Collection option by:

-   pointing to the Pathology Data Collection option and clicking the
    right mouse button

-   using the arrow keys to move the highlight bar to the pathology data
    collection option and pressing enter

    The test selection screen should appear.

6.  You may use the arrow keys to highlight the study number
    **05999/01**.

7.  Press \<**F2\>** to display information on the test with which you
    will be working. When you have completed the review of the displayed
    information, press **ESC** to clear the screen.

8.  You may select the test highlighted by:

-   pressing enter

-   pointing and clicking the right mouse button

    The **SELECT A CURRENT PATHOLOGIST** window appears.

9.  You may choose the Pathologist by

-   pointing and clicking with right mouse button

-   using arrow keys to highlight appropriate Pathologist and press
    enter.

The **PATHOLOGY MAIN MENU** Screen Appears.

Congratulations! You have successfully logged on, chosen your study and
your pathologist. You are now ready to proceed to Section 3. *Assigning
Protocol Required Tissues.*

Assigning Protocol Required Tissues

This section contains three sets of Protocol Required Tissues (PRT). A
set refers to a group of animals that have certain parameters, such as
removal date, removal reason, and specific tissues, in common.

During this practice session you will:

-   select treatment groups and removal reasons

-   assign protocol require tissues

1.  To choose the **ASSIGN PROTOCOL REQUIRED TISSUE** option at the
    **PATHOLOGY MAIN MENU**, you may:

-   use the arrow keys to highlight and press enter

-   point and click the right mouse button

The Protocol Required Tissue (PRT) screen will appear. The Treatment
Group window is on the left, the Removal Reason window is in the middle,
and the Tissue window is on the right.

You are now ready to assign the first of your three PRT sets.

To assign ***Set 1 of 3*** you will:

2.  Press \<**F5\>** to enter Add mode.

Notice the status line at the bottom of the screen changes from

Use arrow keys, F8, or F9 to Review Protocol Require Tissue Data

to

Enter the removal Start Date

Also notice the line in the upper left-hand corner changes from

Review/Delete PRTs

to

Add PRTs

Since this study is a subchronic and we are assigning PRTs, the set
number information in the upper right hand corner should read 1 of 1.
The removal default dates range from [01/01/???? to 12/31/2025.]{.mark}


3.  If you wish to accept the default dates you may:

-   press the enter key twice

-   point and click with the right mouse button

4.  To mark each female treatment group you may:

-   Use the arrow key to highlight the appropriate group and press
    **INSERT**

-   Press the CTRL key while pointing and clicking with the right mouse
    button

    The cursor will advance automatically after each selection.

5.  Press tab when all female treatment groups are marked. The cursor
    will move to the removal window.

    6\. To mark the removal reasons you may:

-   Use the arrow key to highlight the appropriate group and press
    **INSERT**

-   Press the CTRL key while pointing and clicking with the right mouse
    button

    The cursor will advance automatically after each selection.

7.  Continuing in this manner, mark the following removal reasons:

-   Dosing accident

-   Moribund Sacrifice

-   Natural Death

-   Terminal Sacrifice

8.  To move to the Tissue selection screen you may:

9.  To view the entire tissue listing you may:

10. To select the tissues you may:

11. To save the set you have just created you may

To assign ***Set 2 of 3*** you will:

12\. Press \<**F5\>** to enter Add mode.

Notice the status line at the bottom of the screen changes from

Use arrow keys, F8, or F9 to Review Protocol Require Tissue Data

to

Enter the removal Start Date

Also notice the line in the upper left-hand corner changes from

Review/Delete PRTs

to

Add PRTs

Since this study is a subchronic and we are assigning PRTs, the set
number information in the upper right hand corner should read 1 of 1.
The removal default dates range from [01/01/???? to 12/31/2025.]{.mark}


13\. If you wish to accept the default dates you may:

-   press the enter key twice

-   point and click with the right mouse button

    14\. To mark each female treatment group you may:

-   Use the arrow key to highlight the appropriate group and press
    **INSERT**

-   Press the **CTRL** key while pointing and clicking with the right
    mouse button

    The cursor will advance automatically after each selection.

    15\. Press tab when all female treatment groups are marked.

    The cursor will move to the removal window.

    16\. To choose the removal reasons you may:

    17\. Continuing in this manner, mark the following removal reasons:

18\. To view the entire tissue listing you may:

19\. To select the tissues you may:

> 20\. To save the set you have just created you may

Define Observations

Often the same observation is recorded for many animals in a test.
Instead of building the same observation many times, you can build the
observation once and store it for quick recall, using the Define
Observations option.

Define Observations allows you to build an observation and assign a key
combination to it. The observation and its associated key combination
are stored in the test "dictionary." Once an observation is stored, you
may quickly retrieve it either by choosing the observation from the
Select Observation Screen or by pressing the key combination you
assigned to the observation.

NOTE

> When you define an observation, it is available to be assigned to any
> animals in the test, not only for the animals currently being
> observed.

To Define and Store an observation, Follow these Steps:

1.  Highlight **DEFINE OBSERVATIONS** in the Pathology Main Menu and
    press **\<ENTER\>.**

2.  Or, press **\<F3\>** at the Tissue Selection Screen.

3.  The Define Observations Screen appears. Press **\<F4\>.** A list of
    tissues appears. The tissues on this list are suitable for the
    species and include the organs for both sexes.

4.  Use the arrow keys to highlight a tissue. Press **\<ENTER\>.**

5.  The tissue you chose appears in the **TISSUE** field.

6.  To specify site(s), press **\<F5\>.** A list of sites appears.

7.  Use the arrow keys to highlight a site and press **\<ENTER\>.** The
    site you choose appears in the **SITE** field.

8.  Repeat this step if you wish to choose another site. You may choose
    up to three sites.

9.  Press the Function Key associated with the type of morphology you
    wish to enter (**F6---Neoplastic, F7---Non Neoplastic, or
    F8---Metastatic**). A list of selected morphologies appears.

10. Use the arrow keys to highlight a morphology. Press **\<ENTER\>.**
    The morphology you choose appears in the **MORPHOLOGY** field. If
    you chose a **METASTATIC** morphology, a list of tissues for the
    selection of a primary site appears. Highlight the primary site and
    press **\<ENTER\>**. If you choose a **NON-NEOPLASTIC** morphology,
    a list of qualifiers appears. Highlight a qualifier and press
    **\<ENTER\>.**

11. To specify qualifier(s) for a **NEOPLASTIC** or **METASTATIC**
    morphologies, press **\<F9\>.** A list of qualifiers suitable for
    the morphology appears.

12. Use the arrow keys to highlight the qualifier you wish to choose.
    Press **\<ENTER\>.** The qualifier you chose appears in the
    **QUALIFIER** field. To choose another qualifier, repeat step 12.
    You may choose up to four qualifiers.

13. For **NON-NEOPLASTIC** morphologies, if you wish to specify a
    severity qualifier each time you choose this observation during data
    entry, type an **asterisk (\*)** instead of choosing severity
    qualifier(s). Placing an asterisk in the qualifier field will cause
    the system to prompt for a severity qualifier when you choose this
    observation.

14. When you have finished building the observation, you are ready to
    assign a **CONTROL** key combination to the observation.

15. Press **\<F2\>.** A caret **(\^)** appears in the key field. This
    symbol represents the **CTRL** key.

16. Type one letter or two letters that you wish to assign to the
    observation you just defined. If you type one letter, press
    **\<ENTER\>** to complete the key assignment.

17. To retrieve this observation during data entry, press the **CTRL**
    key simultaneously with the letter(s) assigned to the observation.
    For example, if you built an observation for the heart and you
    assigned an **"H"** to the observation, retrieve the observation by
    pressing **\<CTRL-H\>**. Also, if you built an observation for the
    Liver and assigned an **"LB"** to the observation, retrieve the
    observation by pressing **\<CTRL-LB\>.**

18. When you have finished defining the observation, press **\<ESC\>**
    to store the observation in the test "dictionary."

19. If you have finished defining observations, press **\<ESC\>** again.
    You will return to the screen you were on before you created and
    stored the observations.

20. You may retrieve the observation you defined at any time by pressing
    **\<CTRL\>** and the letter(s) you assigned to the observation, or
    by using the Select Observations option.

Building Observations

This section provides practice in:

-   identifying animals

-   selecting tissues

-   entering observations

    To complete this section, follow these steps:

1.  At the Pathology Main Menu, highlight **IDENTIFY ANIMAL** and press
    **\<ENTER\>.**

2.  The Animal Identification Screen appears. Type 20 and press
    \<ENTER\>. The cursor moves to the histology number.

3.  Press **\<ENTER\>.** The following error message appears:

ERROR: Missing Histology Number

> A valid histology number must be entered before proceeding.

You must have an animal number and a histology number to proceed. Press
any key to continue.

4.  Type histology number **H000000008.**

5.  Press **\<F4\>** to review the animal's removal information.

6.  Press **\<ESC\>** to return to the Animal Identification Screen. The
    animal number and histology number that you have entered are
    displayed on the screen.

7.  Press **\<ENTER\>** to begin data entry for animal number **20**.
    The Microscopic Observation Screen appears.

NOTE

> The Microscopic Observations Screen allows you to choose tissues for
> evaluation. The tissues displayed are species and sex specific, and
> are displayed in alphabetical order. If more tissues are available
> than can be displayed on screen, a scroll bar appears on the right
> side of the screen.

8.  In this exercise you will build an observation. Assume you observed
    six small lesions in the brain and diagnosed them as
    **Oligodendroglioma Mal**. Follow these steps to build the
    observation.

9.  Record this observation by using the arrow keys to highlight
    **BRAIN**.

NOTE

> When you build an observation you must specify a morphology.
> Morphologies are divided into three categories: Neoplastic,
> Non-Neoplastic, and Metastatic. The morphologies are tissue and
> sex-specific and are listed alphabetically.

10. Scan the function key options at the bottom of the screen and press
    **\<F6\>** to display the Neoplastic Morphologies window.

11. Highlight **Oligodendroglioma Mal** and press **\<ENTER\>.**

12. Scan the function keys at the bottom of the screen and press
    **\<F9\>** to display the qualifiers window.

NOTE

> Only the qualifier groups suitable to the chosen morphology are
> displayed. In this case, the count qualifiers appear.

13. Highlight multiple and press **\<ENTER\>**.

    *You have successfully built an observation!!!*

14. Press **\<F12\>** to save this observation.

15. Assume the Pituitary Gland is missing and you want to assign the
    missing tissue a status. This time, instead of using the arrow keys
    to highlight the tissue at the Tissue Selection Screen, use the
    following method to build the observation:

16. Type a **P**. The highlighting bar moves to the first term that
    begins with a "**P**."

17. Type an **I.** The highlighting bar moves to the first term that
    begins with a "**PI**."

18. Scan the function key options at the bottom of the screen and press
    **\<F9\>** to display the Tissue Status Screen.

19. Highlight **MISSING** and press **\<ENTER\>** or type the first
    letter of the Tissue Status and the status will automatically be
    added.

20. The Tissue Status window disappears. Notice that the term
    "**Pituitary** **Gland**" has an **"M"** next to it. **"M"** means
    that the tissue has been assigned the status of **MISSING**.

NOTE

> A tissue status of **NORMAL** may be assigned by highlighting the
> tissue and pressing **\<ENTER\>.** Any assigned tissue status may be
> removed by highlighting the tissue and pressing **\<DELETE\>.**

*You have successfully built another observation!*

21. Assume you diagnosed mild, chronic active granulomatous inflammation
    in the heart. Enter this Non-Neoplastic observation by following
    these steps:

22. Highlight the tissue **HEART**.

23. Scan the function key options at the bottom of the screen and press
    **\<F7\>** to display the Non-Neoplastic Morphologies window.

24. Highlight **INFLAMMATION** and press **\<ENTER\>.**

NOTE

> Once the non-neoplastic morphology **"Inflammation"** is selected, a
> window containing the appropriate qualifiers automatically appears.
> This morphology requires qualifiers (determined by the NTP) and the
> system will force you to choose one. If you do not choose a qualifier,
> the system will **[not]{.underline}** add the chosen morphology.

25. Highlight appropriate qualifiers and press **\<ENTER\>.**

    **Mild---Severity qualifier**

    **Chronicactive---Duration qualifier**

    **Granulomatous---Type**

26. Press **\<F12\>** to save this observation.

NOTE

> Only the qualifier groups suitable to the chosen morphology are
> displayed. In this case qualifiers for severity, distribution,
> duration, and inflammation type will be displayed.

27. Assign normal tissue status to the following tissues by highlighting
    the tissues at the Tissue Selection Screen and pressing
    **\<ENTER\>.**

> **Adrenals (cortex and medulla)**
>
> **Bone**
>
> **All Large Intestines (cecum, colon, rectum)**
>
> **All Small Intestines (ileum, jejunum, duodenum)**
>
> **Epididymus**
>
> **Larynx**
>
> **Lung**
>
> **Nose**
>
> **Prostate**
>
> **Testes**
>
> **Seminal Vesicle**

*You have successfully built another observation!*

28. Assume you diagnosed moderate hyperplasia of the thyroid follicular
    cell, a non-neoplastic lesion. Enter this non-neoplastic observation
    by following these steps:

29. Highlight **THYROID** at the Tissue Selection Screen.

30. Scan the function key options at the bottom of the screen and press
    **\<F5\>** to display the Sites window. Sites suitable for the
    selected tissue are listed in alphabetical order.

31. Highlight **FOLLICULAR CELL** and press **\<ENTER\>.**

32. Scan the function key options at the bottom of the screen and press
    **\<F7\>** to display the Non-Neoplastic Morphologies window.

33. Highlight **HYPERPLASIA** and press **\<ENTER\>.**

34. Highlight **MODERATE** and press **\<ENTER\>.**

35. Press **\<F12\>** to save this observation.

36. This time let's try something a little different. Assume during your
    review of the Histopathology Slides, you diagnosed the systemic
    lesion of granulocytic leukemia.

37. Enter this observation using the following steps:

38. Scan the function keys options at the bottom of the screen. Press
    **\<F7\>** to display the Systemic Lesions window.

39. Highlight Leukemia Gran and press **\<ENTER\>.** A tissue window
    appears.

NOTE

> The tissues listed are sex and species specific and are listed
> alphabetically. You may choose all tissues in which the particular
> lesion is present. Tissues that have been previously diagnosed with
> current systemic lesions will appear on the screen already
> highlighted. If the tissue has a corresponding **TGL**, it will be
> flagged with a check mark.

40. Now you will "mark" all the tissues where the systemic lesion
    appears.

41. Highlight **SPLEEN** and press **\<ENTER\>.**This tissue is now
    "marked."

42. Mark the following tissues in the same manner:

> **Thymus**
>
> **Bone Marrow**
>
> **Lymph Node Mesenteric**
>
> **Lymph Node Mandibular**
>
> **Liver**

Press **\<F12\>** to store the observation. The following message
appears:

> **6 observation records were written. Press any key to continue.**

43. Press any key to continue. The Microscopic Observation Screen
    appears.

44. Follow these steps to review the animal:

45. At the Tissue Selection Screen, scan the function key options at the
    bottom of the screen and press **\<ALT-R\>** to display the
    Pathology Data Review Screen.

46. Scroll through the list of observations using the arrow keys or
    **\<PAGE UP\>** and **\<PAGE DOWN\>.**

NOTE

> Tissues that have been assigned a tissue status or morphology are
> displayed alphabetically. If you choose a tissue at the Tissue
> Selection Screen before pressing **\<ALT-R\>,** that tissue will be
> highlighted and displayed at the top of the Pathology Data Review
> Screen. Any other data entered for this animal is displayed above the
> current tissue. The scroll bar displayed at the right side will
> indicate additional data above or below the highlighted tissue.

47. Follow these steps to assign a cause of death.

48. Highlight a tissue and a morphology of your choice and press
    **\<ALT-P\>.** A **"P"** appears on the screen next to the
    morphology you have chosen.

49. Press **\<ESC\>** to return to the Microscopic Observations.

50. Scan the function keys options at the bottom of the screen and press
    **\<F10\>** to complete the animal. The following error message
    appears:

ERROR: Missing Protocol Required Tissue

> Animal 20 cannot be flagged as complete until all protocol required
> tissues have been observed. Press any key to continue.

51. Press any key to continue. You will return to the Microscopic
    Observations Screen.

52. Let's try something a little different. Assume you are during your
    review of the liver, you observed an adhesion and attributed it to
    the mesenteric inflammation. You want to enter this observation as a
    note.

53. Highlight **LIVER** at the Tissue Selection Screen and press
    **\<F2\>.** The Note Selection window appears.

54. Highlight Tissue Note and press **\<ENTER\>.** A note window
    appears.

55. Type the following tissue note for the liver: Adhesion resulted from
    the Mesenteric Inflammation. Review the note. If it is correct,
    press **\<ENTER\>.**

56. The note window disappears and the Microscopic Observations Screen
    appears.

NOTE

> You may enter up to **160** characters and the text will wrap around
> as you type. Press **\<ENTER\>** only when the note is complete.

60. Assume that, while reading slides for this chemical, you diagnosed
    several animals with the same lesions. Follow these steps:

61. At the Microscopic Observations Screen, press **\<F3\>** to display
    the Define Observations Screen.

62. Scan the function keys at the bottom of the screen and perform these
    steps:

63. Press **\<F5\>** to access the Add Observations Screen.

64. Press **\<F4\>** to display a list of tissues.

65. Highlight Kidney and press **\<ENTER\>.**

66. Press **\<F5\>** to display a list of tissue-specific sites.

67. Highlight **Renal Tubule** and press **\<ENTER\>.**

68. Press **\<F7\>** to display a list of Non-Neoplastic Morphologies.

69. Highlight **MINERALIZATION** and press **\<ENTER\>.** A list of
    qualifiers appears because this qualifier has a forced qualifier.

70. Press **\<SHIFT-8\>** to place an asterisk **(\*)** in the severity
    qualifier field. This causes the system to prompt you for a severity
    qualifier each time you assign this observation. Now you are ready
    to assign the unique designator to this diagnosis.

71. Press **\<F2\>.** A caret **(\^)** appears in the key field. This
    symbol represents the control key.

72. Type **KM** (the unique designator) to complete the key assignment.

73. Press **\<F12\>** to build another observation, or press **\<ESC\>**
    to store the observation in the Dictionary. Now each time you wish
    to diagnose mineralization of the renal tubule, you can hold down
    the **\<CTRL\>** key and press **\<K\>** and **\<M\>.**

74. Follow these steps to mark the animal as complete:

75. Press **\<F10\>** at the Microscopic Observations Screen.

The following message appears

> **\*\*Animal has been flagged as complete.\*\***

The Animal Identification Screen appears. You have finished entering
data for this animal.

*You are now ready to use the Raw Data Tables provided with this case
study to practice entering pathology data. A list of all animals in this
case study is provided in **Appendix A.***

Appendix A: Raw Data Tables

PATHOLOGY CASE STUDY: STARDUST 2010 O5-05999-01

> **RAW DATA ANIMAL NUMBER TABLES**

  ------------- ------------- --------------- ----------- ----------------
  **Animal      **Cage        **Removal       **Removal   **Sex**
  Number**      Number**      Reason**        Date**      

  001           1             Terminal Sac    05-12-98    Male

  002           1             Terminal Sac    05-12-98    Male

  003           1             Moribund Sac    03-17-98    Male

  004           1             Terminal Sac    05-12-98    Male

  005           1             Terminal Sac    05-12-98    Male

  006           2             Terminal Sac    05-12-98    Male

  007           2             Terminal Sac    05-12-98    Male

  008           2             Terminal Sac    05-12-98    Male

  009           2             Accidentally    04-19-98    Male
                              Killed                      

  010           2             Terminal Sac    05-12-98    Male

  101           3             Terminal Sac    05-13-98    Female

  102           3             Terminal Sac    05-13-98    Female

  103           3             Terminal Sac    05-13-98    Female

  104           3             Accidentally    05-01-98    Female
                              Killed                      

  105           3             Terminal Sac    05-13-98    Female

  106           4             Terminal Sac    05-13-98    Female

  107           4             Terminal Sac    05-13-98    Female

  108           4             Moribund Sac    05-01-98    Female

  109           4             Terminal Sac    05-13-98    Female

  110           4             Missing         05-01-98    Female

  201           5             Terminal Sac    05-12-98    Male

  202           5             Terminal Sac    05-12-99    Male

  203           5             Terminal Sac    05-12-99    Male

  204           5             Terminal Sac    05-12-99    Male

  205           5             Terminal Sac    05-12-99    Male

  206           6             Terminal Sac    05-12-99    Male

  207           6             Terminal Sac    05-12-99    Male

  208           6             Terminal Sac    05-12-99    Male

  209           6             Missing         05-01-98    Male

  210           6             Terminal Sac    05-12-99    Male

  301           7             Terminal Sac    05-13-99    Female

  302           7             Terminal Sac    05-13-99    Female

  303           7             Terminal Sac    05-13-99    Female

  304           7             Moribund Sac    02-28-98    Female

  305           7             Terminal Sac    05-13-99    Female

  306           8             Terminal Sac    05-13-99    Female

  307           8             Terminal Sac    05-13-99    Female

  308           8             Natural Death   04-28-98    Female

  309           8             Terminal Sac    05-13-98    Female

  310           8             Accidentally    03-28-98    Female
                              Killed                      

  401           9             Terminal Sac    05-12-98    Male

  402           9             Terminal Sac    05-12-98    Male

  403           9             Moribund Sac    05-10-98    Male

  404           9             Terminal Sac    05-12-98    Male

  405           9             Terminal Sac    05-12-98    Male

  406           10            Terminal Sac    05-12-98    Male

  407           10            Terminal Sac    05-12-98    Male

  408           10            Terminal Sac    05-12-98    Male

  409           10            Natural Death   02-24-98    Male

  410           10            Terminal Sac    05-12-98    Male

  501           11            Terminal Sac    05-13-98    Female

  502           11            Terminal Sac    05-13-98    Female

  503           11            Terminal Sac    05-13-98    Female

  504           11            Moribund Sac.   04-01-98    Female

  505           11            Terminal Sac    05-13-98    Female

  506           12            Terminal Sac    05-13-98    Female

  507           12            Terminal Sac    05-13-98    Female

  508           12            Natural Death   04-17-98    Female

  509           12            Terminal Sac    05-13-98    Female

  510           12            Natural Death   04-17-98    Female

  601           13            Terminal Sac    05-12-98    Male

  602           13            Terminal Sac    05-12-98    Male

  603           13            Missing         04-02-98    Male

  604           13            Terminal Sac    05-12-98    Male

  605           13            Terminal Sac    05-12-98    Male

  606           14            Terminal Sac    05-12-98    Male

  607           14            Terminal Sac    05-12-98    Male

  608           14            Terminal Sac    05-12-98    Male

  609           14            Accidentally    03-20-98    Male
                              Killed                      

  610           14            Terminal Sac    05-12-98    Male

  701           15            Terminal Sac    05-13-98    Female

  702           15            Terminal Sac    05-13-98    Female

  703           15            Terminal Sac    05-13-98    Female

  704           15            Natural Death   04-14-98    Female

  705           15            Terminal Sac    05-13-98    Female

  706           16            Terminal Sac    05-13-98    Female

  707           16            Terminal Sac    05-13-98    Female

  708           16            Natural Death   03-03-98    Female

  709           16            Terminal Sac    05-13-98    Female

  710           16            Moribund Sac    03-23-98    Female

  801           17            Terminal Sac    05-12-98    Male

  802           17            Terminal Sac    05-12-98    Male

  803           17            Moribund Sac    03-13-98    Male

  804           17            Terminal Sac    05-12-98    Male

  805           17            Terminal Sac    05-12-98    Male

  806           18            Terminal Sac    05-12-98    Male

  807           18            Terminal Sac    05-12-98    Male

  808           18            Terminal Sac    05-12-98    Male

  809           18            Natural Death   02-10-98    Male

  810           18            Terminal Sac    05-12-98    Male

  901           19            Terminal Sac    05-13-98    Female

  902           19            Terminal Sac    05-13-98    Female

  903           19            Terminal Sac    05-13-98    Female

  904           19            Accidentally    02-27-98    Female
                              Killed                      

  905           19            Terminal Sac    05-13-98    Female

  906           20            Terminal Sac    05-13-98    Female

  907           20            Terminal Sac    05-13-98    Female

  908           20            Moribund Sac    04-07-98    Female

  909           20            Terminal Sac    05-13-98    Female

  910           20            Moribund Sac    04-07-98    Female

  1001          21            Terminal Sac    05-12-98    Male

  1002          21            Terminal Sac    05-12-98    Male

  1003          21            Natural Death   03-14-98    Male

  1004          21            Terminal Sac    05-12-98    Male

  1005          21            Terminal Sac    05-12-98    Male

  1006          22            Terminal Sac    05-12-98    Male

  1007          22            Terminal Sac    05-12-98    Male

  1008          22            Terminal Sac    05-12-98    Male

  1009          22            Moribund Sac    04-17-98    Male

  1010          22            Terminal Sac    05-12-98    Male

  1101          23            Terminal Sac    05-13-98    Female

  1102          23            Terminal Sac    05-13-98    Female

  1103          23            Terminal Sac    05-13-98    Female

  1104          23            Natural Death   05-12-98    Female

  1105          23            Terminal Sac    05-13-98    Female

  1106          24            Terminal Sac    05-13-98    Female

  1107          24            Terminal Sac    05-13-98    Female

  1108          24            Natural Death   4-26-98     Female

  1109          24            Terminal Sac    05-13-98    Female

  1110          24            Natural Death   04-26-98    Female
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