[Curriculum Vitae]{.underline}

[Name]{.underline}: Bradley A. Carlson

[Citizenship]{.underline}: United States

[Education]{.underline}:

1991 B.S. Biological Sciences, Michigan State University, East Lansing,
MI

[Brief Chronology of Employment]{.underline}:

1992‑1996 Biologist, Laboratory of Biological Chemistry, National Cancer
Institute, National Institutes of Health, Bethesda, MD 20892

2003. Biologist, Section on the Molecular Biology of Selenium, Basic
      Research Laboratory, National Cancer Institute, National
      Institutes of Health, Bethesda, MD 20892

2003-Present Biologist, Section on the Molecular Biology of Selenium,
Laboratory of Cancer Prevention, Center for Cancer Research, National
Cancer Institute, National Institutes of Health, Bethesda, MD 20892

[Research Interests]{.underline}:

The role of ribosomal frameshifting in the expression of HIV and other
mammalian retroviruses by *in vitro* and *in vivo* assays and the role
of specific modified tRNAs in this process. The role of selenium in HIV
expression and human health. Analyses of transgenic mice expressing
mutant selenocysteine tRNAs and of mice harboring a conditional knockout
of the selenocysteine tRNA gene. Analysis of the selenocysteine
biosynthesis machinery in eukaryotes.

[Awards]{.underline}:

NIH Merit Award "For discoveries advancing our knowledge of the role of
selenium in health."

October 28, 2004.

[Bibliography]{.underline}

1.  Danilenko, M., Worland, P., Carlson, B., Sausville, E., and
    Sharoni, Y. Selective effects of mastoparan analogs: separation of
    G-protein-directed and membrane-perturbing activities. *Biochem.
    Biophys. Res. Com*. 196: 1296-1302 (1993).

2.  Losiewicz, M.D., Carlson, B.A., Kaur, G., Sausville, E.A., and
    Worland, P.J. Potent inhibition of cdc2 kinase activity by the
    flavonoid L86-8275. *Biochem. Biophys. Res. Com*. 201: 589-595
    (1994).

3.  Wang, Q., Worland, P.J., Clark, J.L., Carlson, B.A., and Sausville,
    E.A. Apoptosis in 7-hydroxystaurosporine-treated T lymphoblasts
    correlates with activation of cyclin-dependent kinases 1 and 2.
    *Cell Growth and Diff.* 6: 927-936 (1995).

4.  Carlson, B.A., Dubay, M.M., Sausville, E.A., Brizuela, L., and
    Worland, P.J. Flavopiridol induces G1 arrest with inhibition of cdk2
    and cdk4 in human breast carcinoma cells. *Cancer Res*. 56:
    2973-2978 (1996).

5.  Sedlacek, H.H., Czech, J., Naik, J., Kaur, G., Worland, P.J.,
    Losiewicz, M., Parker, B., Carlson, B., Smith, A., Senderowicz, A.,
    and Sausville, E.A. Flavopiridol (L86 8275; NSC 649890), a new
    kinase inhibitor for tumor therapy. *Int.* *Journal of Oncology*. 9:
    1143-1168 (1996).

6.  Park, S.I., Park, J.M., Chittum. H.S., Yang, E.S., Carlson, B.A.,
    Lee, B.J., and Hatfield, D.L. Selenocysteine tRNAs as central
    components of selenoprotein biosynthesis in eukaryotes. *Biomed.
    Environ. Sci*. 10: 116-124 (1997).

7.  Chittum, H.S., Hill, K.E., Carlson, B.A., Lee, B.J., Burk, R.F., and
    Hatfield, D.L. Replenishment of selenium deficient rats with
    selenium results in redistribution of the selenocysteine tRNA
    population in a tissue specific manner. *Biochim. Biophys. Acta*.
    1359: 25-34 (1997).

8.  Baek, H.J., Chittum, H.S., Yang, E.S., Park, S.I., Park, J.M.,
    Gladyshev, V.N., Moustafa, M.E., Carlson, B.A., Diamond, A.M., Lee,
    B.J., and Hatfield, D.L. Response of the selenocysteine tRNA
    population to selenium in mammals and *Xenopus* oocytes. *Nucleic
    Acids Symposium Series.* 36: 157-158 (1997).

9.  Chittum, H.S., Lane, W.S., Carlson, B.A., Roller, P.P., Lung,
    F-D.T., Lee, B.J., and Hatfield, D.L. Rabbit B-globin is extended
    beyond its UGA stop codon by multiple suppressions and translational
    reading gaps. *Biochemistry* 38: 10866-10870 (1998).

10. Anderson, J., Pak, M., Phan, L., Carlson, B., Cuesta, R., Asano, K.,
    Tamame, M., and Hinnebusch, A. A nuclear complex containing Gcd10p
    and Gcd14p stimulates translation by promoting modification and
    maturation of initiator methionyl-tRNA. *Genes and Development* 12:
    3650-62 (1998).

11. Huh, J.R., Park, J.M., Carlson, B., Hatfield, D., and Lee, B.J.
    Recruitment of TBP or TFIIB to a promoter proximal position leads to
    the stimulation of RNA Polymerase II transcription without activator
    proteins both *in vivo* and *in vitro*. *Biochem Biophys Res Commun*
    256: 45-51 (1999).

12. Carlson, B.A., Kwon, S.Y., Chamorro, M., Oroszlan, S., Hatfield, D.,
    and Lee, B.J. Transfer RNA modification status influences retroviral
    ribosomal frameshifting. *Virology* 255: 2-8 (1999).

13. Zhou, X., Park, S.I., Carlson, B.A., Moustafa, M.E., Crain, P.F.,
    Jung, J.-E., Diamond, A.M., Hatfield, D.L. and Lee, B.J. Selenium
    metabolism in *Drosophila*: Characterization of the selenocysteine
    tRNA population*. J Biol. Chem.* 274: 18729-18734 (1999).

14. Xu, X.-M., Carlson, B.A., Kim, L.K., Lee, B.J., Hatfield, D.L., and
    Diamond, A.M. Analysis of selenocysteine tRNA^\[Ser\]Sec^ genes in
    Chinese hamsters. *Gene* 239: 49-53 (1999).

15. Xu, X.-M., Zhou, X., Carlson, B., Kim, L.K., Huh, T-l., Lee, B.J.
    and Hatfield, D.L. Zebrafish genome contains two distinct
    selenocysteine (Sec) tRNA^\[Ser\]Sec^ genes. *FEBS Letters* 454:
    16-20 (1999).

16. Carlson, B., Lahusen, T., Singh, S., Loaiza-Perez, A., Worland, P.,
    Pestell, R., Albanese, C., Sausville, E., and Senderowicz, A.
    Down-regulation of cyclin D1 by transcriptional repression in MCF-7
    human breast carcinoma cells induced by Flavopiridol. *Cancer Res.*
    59: 4634-4641 (1999).

17. Kumaraswamy, E., Xu, X-M., Martin-Romero, F.J., Carlson, B.A., and
    Hatfield, D.L. Roles of UGA in mammalian protein synthesis:
    selenocysteine, stop suppression and reading gaps. Review. *Current
    Topics in Biochemical Research* 1: 113-123 (1999).

18. Gladyshev, V.N., Martin-Romero, F.J., Xu, X.-M., Kumaraswany, E.,
    Carlson, B.A., Hatfield, D.L. and Lee, B.J.: Molecular biology of
    selenium and its role in cancer, AIDS and other human diseases.
    *Recent Research Developments in Biochemistry* 1: 145-167 (1999).

19. Hatfield, D., Gladyshev, V., Park, J., Park, S., Chittum, H., Huh,
    J., Carlson, B., Kim, M., Moustafa, M., Lee, B.J. Biosynthesis of
    selenocysteine and its incorporation into protein as the 21^st^
    amino acid. *In* Comprehensive Natural Products Chemistry. Elsevier
    Sc. Ltd. Volume 4, 353-380 pp. (1999).

20. Mansur, D.B., Hao, H., Gladyshev, V.N., Korotkov, K.V., Hu, Y.,
    Moustafa, M.E., Carlson, B.A., Hatfield, D.L. and Diamond, A.M.
    Multiple Levels of Regulation of Selenoprotein Biosynthesis Revealed
    From the Analysis of Human Glioma Cell Lines. *Biochem. harmacol.*
    60: 489-97 (2000).

21. Copeland, P., Fletcher, J., Carlson, B., Hatfield, D., and
    Driscoll, D. Identification of a novel mammalian RNA binding protein
    required for the cotranslational incorporation of selenocysteine.
    *EMBO J.* 19: 306-314 (2000).

22. Dettenhofer, M., Cen, S., Carlson, B.A., Kleiman, L., and Yu, X-F.
    Association of human immunodeficiency virus type 1 Vif with RNA and
    its role in reverse transcription. *J Virol.* 74: 8938-8945 (2000).

23. Warner, G., Berry, M.J., Moustafa, M.E., Carlson, B.A., Hatfield,
    D.L., and Faust, J.R. Inhibition of selenoprotein synthesis by
    selenocysteine tRNA^\[Ser\]Sec^ lacking isopentenyladenosine. *J
    Biol. Chem.* 275: 28110-28119 (2000).

24. Carlson, B.A., Kwon, S.Y., Lee, B.J., and Hatfield, D. Yeast
    Asparagine (Asn) tRNA without Q base promotes eukaryotic
    frameshifting more efficiently than mammalian Asn tRNAs with or
    without Q base. *Molecules and Cells* 10: 113-118 (2000).

25. Kwon, S.Y., Carlson, B.A., Park, J.M., and Lee, B.J. Structural
    Organization and Expression of the Gaegurin4 Gene of Rana rugosa*.*
    *Biochem Biophys Acta* 1492: 185-190 (2000).

26. Tujebajeva, R.M., Copeland, P.R., Xu, X., Carlson, B.A., Harney,
    J.W., Driscoll, D.M., Hatfield, D.L., and Berry, M.J.. Decoding
    apparatus for eukaryotic selenocysteine insertion. *EMBO Reports* 1:
    158-163 (2000).

27. Kim, L.K., Matsufuji, T., Matsufuji, S., Carlson, B.A., Kim, S.S.,
    Hatfield, D.L., and Lee, B.J. Methylation of the robosyl moiety at
    position 34 of selenocysteine tRNA^\[Ser\]Sec^ is governed by both
    primary and tertiary structure. *RNA* 6: 1306-1315 (2000).

28. Kumaraswamy, E., Malykh, A., Korotkov, K.V., Kozyavkin, S., Hu, Y.,
    Kwon, S.Y., Moustafa, M.E., Carlson, B.A., Berry, M.J., Lee, B.J.,
    Hatfield, D.L., Diamond, A.M., and Gladyshev, V.N.
    Structure-expression relationships of the 15 kDa selenoprotein gene:
    possible role of the protein in cancer etiology. *J Biol. Chem.*
    275: 35540-35547 (2000).

29. Carlson, B.A., Mushinski, J.F., Henderson, D.W., Kwon, S.Y., Crain,
    P.F., Lee, B.J., and Hatfield, D.L. 1-methylguanosine in place of Y
    base at position 37 in phenylalanine tRNA is responsible for its
    shiftiness in retroviral ribosomal frameshifting. *Virology* 279:
    130-135 (2001).

30. Carlson, B.A., Martin-Romero, F.J., Kumaraswany, E., Moustafa, M.E.,
    Zhi, H., Hatfield, D.L and Lee, B.J.: Mammaliam selenoysteine tRNA.
    In Selenium: Its molecular biology and role in human health. D.L.
    Hatfield, Editor. Chapter 3, p. 23-32, 2001. Kluwer Academic
    Publishers, Norwell, MA, pp. 326 (2001).

31. \*Moustafa, M.E., \*Carlson, B.A., El-Saadani, M.A., Kryukov, G.V.,
    Sun, Q.A., Harney, J.W., Hill, K.E., Burk, R.F., Combs, G.F.,
    Feigenbaum, L., Mansur, D.B., Berry, M.J., Diamond, A.M., Gladyshev,
    V.N., Lee, B.J., and Hatfield, D.L. Selective inhibition of
    selenocysteine tRNA maturation and selenoprotein synthesis in
    transgenic mice expressing an isopentenyladenosine mutant
    selenocysteine tRNA^\[Ser\]Sec^ transgene. *Mol. and Cell. Biol.*
    21: 3840-3852 (2001).

32. Martin-Romero, F.J., Kryukov, G.V., Lobanov, A.V., Carlson, B.A.,
    Lee, B.J., Gladyshev, V.N., and Hatfield, D.L. Role of selenium in
    aging: selenoproteins, selenoprotein mRNA expression, fertility and
    mortality in Drosophila. *J. Biol. Chem.* 276: 29798-804 (2001).

33. Berry, M.J., Tujebajeva, R.M., Copeland, P.R., Xu, X.M., Carlson,
    B.A., Martin, G.W., Low, S.C., Mansell, J.B., Grundner-Culemann, E.,
    Harney, J.W., Driscoll, D.M., Hatfield, D.L. Selenocysteine
    incorporation directed from the 3\'UTR: characterization of
    eukaryotic EFsec and mechanistic implications. *Biofactors* 14:
    17-24 (2001).

34. Carlson, B.A., and Hatfield, D.L. Transfer RNAs that insert
    selenocysteine. *Methods Enzymol.* 347: 24-39 (2002).

35. \*Jameson, R.R., \*Carlson, B.A., Butz, M., Esser, K., Hatfield,
    D.L., and Diamond, A.M. Selenium influences the turnover of
    selenocysteine tRNA^\[Ser\]Sec^ in chinese hamster ovary cells. *J.
    Nutr.* 132: 1830-1835 (2002).

36. \*Xu, X.-M., \*Carlson, B.A., Grimm, T.,Kutza, J., Berry, M.J.,
    Arreola, R., Fields, K., Shanmugam, I., Jeang, K.-T., Oroszlan, S.,
    Combs, G.F., Marx, P.A., Gladyshev, V.N., Clouse, K.A., and
    Hatfield, D.L. Rhesus monkey simian immunodeficiency virus infection
    as a model for assessing the role of selenium in AIDS. *J. Acquir.
    Immune Defic. Syndr.* 31: 453-463 (2002).

37. Kim, M., Park, C.H., Lee, M.S., Carlson, B.A., Hatfield, D.L.and
    Lee, B.J. A novel TBP-interacting zinc finger protein represses
    transcription by inhibiting the recruitment of TFIIA and TFIIB.
    *Biochem. Biophys. Res. Comm.* 306: 231-238 (2003).

38. Kim, M., Choi, J., Carlson, B.A., Han, J.K., Rhee, K., Sargent, T.,
    Hatfield, D.L., and Lee, B.J. A novel TBP-interacting zing finger
    protein is related in the neural development of *Xenopus laevis*.
    *Biochem. Biophys. Res. Comm* 306: 1106-1111 (2003).

39. Kumaraswamy, E., Carlson, B.A., Morgan, F., Miyoshi, K., Robinson,
    G., Su, D., Wang, S., Southon, E., Tessarollo, L., Lee, B.J.,
    Gladyshev, V.N., Hennighausen, L., and Hatfield, D.L. Selective
    removal of the selenocysteine tRNA\[Ser\]Sec gene (Trsp) in mouse
    mammary epithelium. *Mol. Cell. Biol.* 23: 1477-1488 (2003).

40. Moustafa M.E., Kumaraswamy, E., Zhong, N., Rao, M., Carlson, B.A.,
    and Hatfield, D.L. Models for assessing the role of selenoproteins
    in health. *J. Nutr.* 133: 2494S-2496S (2003).

41. Rao, M., Carlson, B.A., Novoselov, S.V., Weeks, D.P., Gladyshev,
    V.N., and Hatfield, D.L. Chlamydomonas reinhardtii selenocysteine
    tRNA\[Ser\]Sec. *RNA* 9(8): 923-930 (2003).

42. Hornberger, T.A., McLoughlin, T.J., Leszczynski, J.K., Armstrong,
    D.D., Jameson, R.R., Bowen, P.E., Hwang, E.S., Hou, H., Moustafa,
    M.E., Carlson, B.A., Hatfield, D.L., Diamond, A.M., Esser, K.A.
    Selenoprotein-deficient transgenic mice exhibit enhanced
    exercise-induced muscle growth. *J. Nutr.* 133(10): 3091-3097
    (2003).

43. Kwon, S.Y., Badenhorst, P., Martin-Romero, F.J., Carlson, B.A.,
    Paterson, B.M., Gladyshev, V.N., Lee, B.J., and Hatfield, D.L. The
    Drosophila Selenoprotein BthD Is Required for Survival and Has a
    Role in Salivary Gland Development. *Mol. Cell. Biol.* 23(23):
    8495-8504 (2003).

44. Carlson, B.A., Novoselov, S.V., Kumaraswamy, E., Anver, M.R.,
    Gladyshev, V.N., and Hatfield, D.L. Specific excision of the
    selenocysteine tRNA^\[Ser\]Sec^ gene in mouse liver demonstrates an
    essential role of selenoproteins in liver function. *J. Biol. Chem.*
    279(9): 8011-8017 (2004).

45. Carlson, B.A., Xu, X.M., Kryukov, G.V, Rao, M., Berry, M.J.,
    Gladyshev, V.N., and Hatfield, D.L. Identification and
    characterization of phosphoseryl-tRNA^\[Ser\]Sec^ kinase. *Proc.
    Natl. Acad. Sci. USA* 101(35): 12848-12853 (2004).

46. Shrimali, R.K., Lobanov, A.V., Xu, X.M., Rao, M., Carlson, B.A.,
    Mahadeo, D.C., Parent, C.A., Gladyshev, V.N., and Hatfield, D.L.
    Selenocysteine tRNA identification in the model organisms
    Dictyostelium discoideum and Tetrahymena thermophila. *Biochem.
    Biophys. Res. Commun.* 329(1): 147-51 (2005).

47. Schweizer, U., Streckfuss, F., Pelt, P., Carlson, B.A., Hatfield,
    D.L., Kohrle, J., and Schomburg, L. Hepatically derived
    selenoprotein P is a key factor for kidney but not for brain
    selenium supply. *Biochem. J.* 386: 221-226 (2005).

48. Carlson, B.A., Xu, X.M., Gladyshev, V.N., and Hatfield. D.L.
    Selective rescue of selenoprotein expression in mice lacking a
    highly specialized methyl group in selenocysteine tRNA. *J. Biol.
    Chem.* 280(7): 5542-5548 (2005).

49. Carlson, B.A., Xu, X.M., Gladyshev, V.N., and Hatfield, D.L. Um34 in
    selenocysteine tRNA is required for the expression of stress-related
    selenoproteins in mammals. In Grosjean (Ed.): Fine-Tuning of RNA
    Functions by Modification and Editing, *Topics in Current Genetics*,
    Vol. 12. pp 431-438 (2005).

50. Novoselov, S.V., Calvisi, D., Labunskyy, V.M., Factor, V.M.,
    Carlson, B.A., Fomenko, D.E., Moustafa, M.E., Hatfield, D.L. and
    Gladyshev, V.N. Selenoprotein deficiency and high levels of selenium
    compounds can effectively inhibit hepatocarcinogenesis in transgenic
    mice. *Oncogene* 24(54): 8003-8011 (2005).

51. Sun, Q.A., Su, D., Novoselov, S.V., Carlson, B.A., Hatfield, D.L.,
    and Gladyshev, V.N. Reaction mechanism and regulation of mammalian
    thioredoxin/glutathione reductase. *Biochemistry* 44(44):
    14528-14537. (2005).

52. Xu, X.M., Mix, H., Carlson, B.A., Grabowski, P.J., Gladyshev, V.N.,
    Berry, M.J., and Hatfield, D.L. Evidence for direct roles of two
    additional factors, SECp43 and soluble liver antigen, in the
    selenoprotein synthesis machinery. *J Biol Chem.* 280(50):
    41568-41575. (2005).

53. Hatfield, D.L., Xu, X.-M., Carlson, B.A., Zhong, N. and Gladyshev,
    V.N. Selenocysteine incorporation machinery and the role of
    selenoproteins in health. *Prog. Nucl. Acid Res. Mol. Biol.* 81:
    97-142. (2006).

54. Chen, C.L., Shim, M.S., Chung, J., Yoo, H.S., Ha, J.M., Kim, J.Y.,
    Choi, J., Zang, S.L., Hou, X. Carlson, B.A., Hatfield, D.L., and
    Lee, B.J. G-rich, a Drosophila selenoprotein, is a Golgi-resident
    type III membrane protein. *Biochem. Biophys. Res. Commun.* 348(4):
    1296-1301. (2006).

55. Irons, R., Carlson, B.A., Hatfield, D.L., and Davis, C.D. Both
    selenoproteins and low molecular weight selenocompounds reduce colon
    cancer risk in mice with genetically impaired selenoprotein
    expression. *J. Nutr.* 136(5): 1311-1317. (2006).

56. Yoo, M.H., Xu, X.M., Carlson, B.A., Gladyshev, V.N., and Hatfield,
    D.L. Thioredoxin reductase 1 deficiency reverses tumor phenotype and
    tumorigenicity of lung carcinoma cells. *J. Biol. Chem.* 281(19):
    13005-13008. (2006).

57. Small-Howard, A., Morozova, N., Stoytcheva, Z., Forry, E.P.,
    Mansell, J.B., Harney, J.W., Carlson, B.A., Xu, X.M., Hatfield,
    D.L., and Berry, M.J. Supramolecular complexes mediate
    selenocysteine incorporation in vivo. *Mol. Cell. Biol.* 26(6):
    2337-2346. (2006).

58. Shrimali, R.K., Weaver, J.A., Miller, G.F., Starost, M.F., Carlson,
    B.A., Novoselov, S.V., Kumaraswamy, E., Gladyshev, V.N., and
    Hatfield, D.L. Selenoprotein expression is essential in endothelial
    cell development and cardiac muscle function. *Neuromuscul. Disord.*
    In Press (2006).

59. \*Xu, X.M., \*Carlson, B.A., Mix, H., Zhang, Y., Saira, K., Glass,
    R.S., Berry, M.J., Gladyshev, V.N., and Hatfield, D.L. Biosynthesis
    of selenocysteine on its tRNA in eukaryotes. *PLoS Biol.* 5(1): In
    Press (2007).

Articles published independently of supervisor.

Article reviewed in [News and Views]{.underline} in *Nature*, Atkins,
J.F. and Gesteland R.F. Translation: The twenty-first amino acid.
*Nature* 407: 463-464 (2000).

Corresponding author articles.

\* Articles in which equal contributions were made and therefore are
first author papers.

Figure associated with this article appeared on July 2001 cover of
Molecular and Cellular Biology, Volume 21, Issue 13.

Article highlighted on the journal's cover.

[MANUSCRIPTS SUBMITTED]{.underline}

1.  Carlson, B.A., Moustafa, M.E., Shrimali, R.K., Rao, M., Wang. S.,
    Feigenbaum, L., Lee, B.J., Gladyshev, V.N. and Hatfield, D.L.
    Partial restoration of the selenoprotein population in a mouse
    hepatocyte selenoproteinless background using two selenocysteine
    tRNA\[Ser\]Sec mutants.

2.  Dikiy, A., Novoselov, S., Fomenko, D., Sengupta, A., Carlson, B.,
    Cerny, R., Ginalski, K., Grishin, N., Hatfield, D., and
    Gladyshev, V. Genomics and molecular insights into the functions of
    selenoproteins of a novel thioredoxin-like family.

3.  Roth, S., Wirth, E., Vogt, J., Brauer, A., Carlson, B., Renko, K.,
    Moskovitz, J., Schneider, I., Tirsch, W., Klopstock, T., Holter, S.,
    Fucks, H., Gailus-Durner, V., de Angelis, M.H., Grune, T., Hatfield,
    D., Kohrle, J., Schomburg, L., Schweizer, U. Impaired expression of
    selenoproteins in the brain leads to neurodegeneration with
    neurological and behavioural abnormalities.

4.  Xu, X.M., Carlson, B.A., Irons, R., Mix, H., Zhong, N., Gladyshev,
    V.N., and Hatfield, D.L. Selenophosphate synthetase 2 is essential
    for selenoprotein biosynthesis.

    [ABSTRACTS]{.underline}

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1.  Carlson, B.A., Sausville, E.A., and Worland, P.J. Flavopiridol
    induced G1-S phase block in MDA-MB-468 breast carcinoma cells with
    deregulation of cdk2. American Association for Cancer Research
    Annual Meeting, Toronto, Ontario, Canada, 1995. Poster presentation.

2.  Wang, Q., Carlson, B.A., Clark, J., Sausville, E.A., and Worland,
    P.J. Activation of CDK1 and CDK2 by UCN-01, a selective PKC
    antagonist is associated with growth inhibition and induction of
    apoptosis in leukemic T-cells. American Association for Cancer
    Research Annual Meeting, Toronto, Ontario, Canada, 1995.

3.  Losiewicz, M.D., Carlson, B.A., Sausville, E.A., Naik, R.G.,
    Narayanan, V.L., and Worland, P.J. Inhibition of cdk2 by
    flavopiridol and structural analogs. American Association for Cancer
    Research Annual Meeting, Toronto, Ontario, Canada, 1995.

4.  Senderowicz, A.M., Carlson, B.A., Malspeis, L., Sausville, E.A., and
    Worland, P.J. Rapamycin induces Rb hypophosphorylation and G1/S
    block in the PC3 prostate carcinoma line. American Association for
    Cancer Research Annual Meeting, Toronto, Ontario, Canada, 1995.

5.  Senderowicz, A.M., Carlson, B.A., Sausville, E.A., and Worland, P.J.
    Rapamycin causes decline in CDK4 activity associated with G1/S block
    in the PC3 prostate carcinoma cell line. American Association for
    Cancer Research Annual Meeting, Washington, D.C., 1996.

6.  Carlson, B.A., Pearlstein, R.A., Naik, R.G., Sedlecek, H.H.,
    Sausville, E.A., and Worland, P.J. Inhibition of cdk2, cdk4, and
    cdk7 by flavopiridol and structural analogs. American Association
    for Cancer Research Annual Meeting, Washington, D.C., 1996. Poster
    presentation.

7.  Park, S.I., Park, J.M., Chittum, H.S., Baek, H.J., Gladyshev, V.N.,
    Yang, E.S., Carlson, B.A., Crain, P.F., Hill, K.E., Burk, R.F.,
    Choi, I.S., Moustafa, M.E., and Lee, B.J. Selenocysteine (sec) tRNAs
    as central components of sec insertion into protein. 17^th^ Annual
    tRNA Workshop, Chiba, Japan, 1997.

8.  Chittum, H., Carlson, B., Hill, K., Burk, R., Lee, B., and
    Hatfield, D. Analysis of the selenocysteine (sec) tRNA population in
    selenium (Se)-deficient rat tissues resupplemented with Se.
    Experimental Biology '97 Annual Meeting, New Orleans, LA, 1997.

9.  Hatfield, D., Park, S., Park, J., Chittum, H., Beck, H., Gladyshev,
    V., Yang, E., Carlson, B., Choi, I., Moustafa, M. and Lee, B.
    Expression, biosythesis and function of selenocysteine (sec) tRNAs
    as central components of sec insertion into protein. Experimental
    Biology '97 Annual Meeting, New Orleans, LA, 1997.

10. Carlson, B.A., Kwon, S.Y., Chamorro, M., Oroszlan, S., Lee, B.J.,
    and Hatfield, D. Retroviral ribosomal frameshifting is enhanced by
    hypomodified tRNAs. Experimental Biology 1998, Washington,
    D.C., 1998. Poster presentation.

11. Carlson, B., Kwon, S.Y., Chamorro, M., Oroszlan, S., Lee, B.J. and
    Hatfield, D. Role of tRNA in vertebrate retroviral ribosomal
    frameshifting: tRNA status influences the frameshift event. NIH
    Research Festival, October, 1998. Poster presentation.

12. Chittum, H., Lane, W., Carlson, B., Roller, P., Lung, F., Lee, B.
    and Hatfield, D. Rabbit Beta-Globin readthrough protein is extended
    beyond Its UGA stop codon by multiple suppressions and ribosomal
    hopping. NIH Research Festival, October, 1998. Poster presentation.

13. Zhou, X., Park, S., Carlson, B., Moustafa, M., Crain, P., Jung,
    J.-E., Lee, B.J., Diamond, A., and Hatfield, D. Characterization of
    selenocysteine (Sec) tRNA population in *Drosophila*. Experimental
    Biology '98 Annual Meeting, Washington, D.C., 1998.

14. Lee, B.J., Park, J.M., Kim, M.J., Kwon, S.Y., Carlson, B.A., and
    Hatfield, D.L. Purification and characterization of Xenopus RNA
    polymerase III and its transcription initiation factors.
    Experimental Biology '98 Annual Meeting, Washington, D.C., 1998.

15. Carlson, B., Kwon, S.Y., Chamorro, M., Oroszlan, S., Lee, B.J., and
    Hatfield, D. Role of tRNA in retroviral ribosomal frameshifting:
    tRNA status influences the frameshift event. 4^th^ International
    Symposium on the Impact of Biotechnology on Cancer Prediction,
    Prevention and Treatment, Nice, France, 1998.

16. Moustafa, M., Carlson, B., Lee, B.J., El-Saadani, M., Kandeel, K.,
    Feigenbaum, L., Diamond, A. and Hatfield, D. Overexpression of
    selenocysteine (Sec) tRNA in transgenic mice. Biochemistry and
    Molecular Biology \'99, San Francisco, CA, 1999. Poster
    presentation.

17. Moustafa, M., Carlson, B., Lee, B.J., El-Saadani, M., Kandeel, K.,
    Feigenbaum, L., Diamond, A. and Hatfield, D. Variation of
    selenocysteine (Sec) tRNA^\[Ser\]Sec^ expression in different
    tissues of transgenic mice. NIH Research Festival, October 7, 1999.
    Poster presentation.

18. Moustafa M.E., Carlson, B.A., Lee, B.J., Gladyshev, V.N., Diamond,
    A.M., and Hatfield, D.L. Overexpression of selenocysteine
    tRNA^\[Ser\]Sec^ in transgenic mice. 18^th^ tRNA Workshop \"tRNA
    2000\" Cambridge, UK, April 8-12, 2000. Poster presentation.

19. Kim, M., Kim, S.S., Kim, L.K., Matsufuji, T., Matsufuji, S.,
    Carlson, B.A., Hatfield, D.L., and Lee, B.J. Methylation of the
    ribosyl moiety at position 34 of selenocysteine tRNA^\[Ser\]Sec^ is
    governed by both primary and tertiary structure. 18^th^ tRNA
    Workshop \"tRNA 2000\", Cambridge, UK, April 8-12, 2000.

20. Carlson, B.A., Henderson, D.W., Kwon, S.Y., Mushinski, J.F., Crain,
    P.F., Lee, B.J., and Hatfield, D.L. Modification status of Phe tRNA
    anticodon loop influences level of retroviral ribosomal
    frameshifting. NIH Research Festival, June, 2000. Poster
    presentation.

21. Carlson, B.A., Henderson, D.W., Kwon, S.Y., Mushinski, J.F., Crain,
    P.F., Lee, B.J., and Hatfield, D.L. Modification status of Phe tRNA
    anticodon loop influences level of retroviral ribosomal
    frameshifting. Translational Control, Cold Spring Harbor, NY,
    September 6-10, 2000. Poster presentation.

22. Berry, M.J., Touebaeva, R., Xu, X.M., Carlson, B.A., Harney, J.,
    Copeland, P., Driscoll, D., and Hatfield, D.L. Selenocysteine
    incorporation directed from the 3\' UTR: characterization of
    eukaryotic SELB. 7^th^ International Symposium on Selenium in
    Biology and Medicine \"Selenium 2000\", Venezia, Italy, October 1-5,
    2000.

23. Moustafa, M.E., Carlson, B.A., Sun, Q.A., Kryukov, G.V., Diamond,
    A.M., Gladyshev, V.N., Lee, B.J., and Hatfield, D.L. Selective
    inhibition of Sec tRNA maturation and selenoprotein synthesis in
    transgenic mice expressing an i^6^A-deficient Sec tRNA gene. 7^th^
    International Symposium on Selenium in Biology and Medicine
    \"Selenium 2000\", Venezia, Italy, October 1-5, 2000.

24. Carlson, B.A., Mushinski, J.F., Kwon, S.Y., Henderson, D.W., Crain,
    P.F., Lee, B.J., and Hatfield, D.L. Role of anticodon loop of Asn
    and Phe tRNAs in retroviral ribosomal frameshifting. Experimental
    Biology 2001, Orlando, FL, March 30-April 4, 2001. Poster
    presentation.

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25. Moustafa, M.E., Carlson, B.A., Kryukov, G.V., Sun, Q-A., Diamond,
    A.M., Lee, B.J., Gladyshev, V.N., and Hatfield, D.L. Transgenic mice
    expressing i6A mutant Sec tRNA^\[Ser\]Sec^: selective inhibition of
    Sec tRNA^\[Ser\]Sec^ maturation and selenoprotein synthesis.
    Experimental Biology 2001, Orlando, FL, March 30-April 4, 2001.

26. Moustafa, M.E., Carlson, B.A., El-Saadani, M.A., Rao, M.,
    Feigenbaum, L., and Hatfield, D.L. Selenocysteine (Sec)
    tRNA^\[Ser\]Sec^ and selenoprotein levels in transgenic mice
    expressing Sec tRNA^\[Ser\]Sec^ with a mutation in the wobble
    position. Experimental Biology 2002, New Orleans, LA, April 20-April
    24, 2002.

27. Carlson, B.A., Kumaraswamy, E., Wang, S., Southon, E., Tessarollo,
    L., Gladyshev, V.N., and Hatfield, D.L. Conditional knockout of the
    selenocysteine (Sec) tRNA gene in mice. Experimental Biology 2002,
    New Orleans, LA, April 20-April 24, 2002. Poster presentation.

28. Kwon, S.Y., Wei, Q., Paterson, B.M., Carlson, B.A., Lee, H.S.,
    Martin-Romero, F.J., Gladyshev, V.N., and Hatfield, D.L. Functional
    studies of *Drosophila* selenoproteins: selenophosphate synthetase 2
    (SPS2), G-rich and BthD. Experimental Biology 2002, New Orleans, LA,
    April 20-April 24, 2002.

29. Rao, M., Kumaraswamy, E., Shanmugam, I., Carlson, B.A., Diamond,
    A.M., and Hatfield, D.L. Multiple forms of selenocysteine (Sec)
    tRNAs may be due to distinct higher order structures. Experimental
    Biology 2002, New Orleans, LA, April 20-April 24, 2002.

30. E. Kumaraswamy, Carlson, B.A., Morgan, F., Miyoshi, K., Robinson,
    G., Su, D., Wang, S., Southon, E., Tessarollo, L., Gladyshev, V.N.,
    Hennighausen, L., and Hatfield, D.L. Selective Removal Of The
    Selenocysteine tRNA^\[Ser\]Sec^ Gene (*Trsp*) In Mouse Mammary
    Epithelium. NIH Research Festival, June, 2002. Poster presentation.

31. Carlson, B.A., Novoselov, S.V., Kumaraswamy, E., Gladyshev, V.N.,
    and Hatfield, D.L. Selective removal of the selenocysteine tRNA gene
    (*Trsp*) in mouse tissues. tRNA World 2003, Banz, Germany, October
    3-7, 2003. Poster presentation.

32. Carlson, B.A., Novoselov, S.V., Kumaraswamy, E., Anver, M.,
    Gladyshev, V.N., and Hatfield, D.L. Specific excision of the
    selenocysteine tRNA^\[Ser\]Sec^ gene in mouse liver demonstrates an
    essential role of selenoproteins in liver function. NIH Research
    Festival, October, 2003. Poster presentation.

33. Carlson, B.A., Xu, X.M., Kryukov, G.V, Rao, M., Berry, M.J.,
    Gladyshev, V.N., and Hatfield, D.L. Identification and
    characterization of phosphoseryl-tRNA^\[Ser\]Sec^ kinase. NIH
    Research Festival, September 29, 2004. Poster presentation.

34. Carlson, B.A., Xu, X.M., Kryukov, G.V, Rao, M., Berry, M.J.,
    Gladyshev, V.N., and Hatfield, D.L. Identification and
    characterization of phosphoseryl-tRNA^\[Ser\]Sec^ kinase.
    Experimental Biology 2005. San Diego, CA, April, 2005. Poster
    presentation.

35. Carlson, B.A., Moustafa, M.E., Gladyshev, V.N., and Hatfield, D.L.
    Novel role of selenocysteine tRNA Um34 in expression of
    stress-related, but not housekeeping, selenoproteins. NCI Postdoc
    Retreat, Ocean City, MD, March, 2006. Poster presentation.

36. Carlson, B.A., Shrimali, R.K., Irons, R., Park, J.M., and Hatfield,
    D.L. Targeted removal of the selenocysteine tRNA^\[Ser\]Sec^ gene
    (*trsp*) in mouse macrophages. Experimental Biology 2007,
    Washington, DC, March, 2007.

37. Anton A. Turanov, Sebastian Kehr, Bradley A. Carlson, Dolph L.
    Hatfield and Vadim N. Gladyshev. Mammalian thioredoxin reductases:
    roles in redox homeostasis and analysis of cellular targets.
    Experimental Biology 2007, Washington, DC, March, 2007.

[INVITED TALKS]{.underline}

1.  Carlson, B.A., Kwon, S.Y., Zhou, X., Chamorro, M., Oroszlan, S.,
    Lee, B.J., and Hatfield, D. Retroviral ribosomal frameshifting is
    influenced by tRNA status. International Conference on Medical
    Virology, Beijing, China, October 22, 1998. Invited speaker.

2.  Carlson, B.A., Kwon, S.Y., Henderson, D., Mushinski, J.F., Crain,
    P., Lee, B.J., and Hatfield, D.L. Role of Q base and Y base in
    retroviral ribosomal frameshifting. 18^th^ tRNA Workshop \"tRNA
    2000\" Cambridge, UK, April 8-12, 2000. Invited speaker.

3.  Carlson, B.A., Kumaraswamy, E., Su, D., Lee, B.J., Gladyshev, V.N.,
    and Hatfield, D.L. Targeted removal of the selenocysteine
    tRNA^\[Ser\]Sec^ gene (Trsp) in mouse mammary tissues. Experimental
    Biology 2003. San Diego, CA April 11-15, 2003. Invited speaker.

4.  Carlson, B.A., Xu, X.-M, Gladyshev, V.N., and Hatfield, D.L.
    Selective rescue of selenoproteins expression in mice. Experimental
    Biology 2004. Washington, DC April, 2004. **Special Invite.**

5.  Carlson, B.A., Gladyshev, V.N., and Hatfield, D.L. Partial
    replacement of the selenoprotein population in mouse liver as a
    model for elucidating the role of selenoproteins in health.
    Experimental Biology 2005. San Diego, CA, April, 2005. Invited
    speaker.

6.  Carlson, B.A., Moustafa, M.E., Gladyshev, V.N., and Hatfield, D.L.
    Novel role of selenocysteine tRNA Um34 in expression of
    stress-related, but not housekeeping, selenoproteins. 21^st^ tRNA
    Workshop, December 2-7, 2005, Bangalore, India. Invited speaker.
    Unable to attend.

7.  Bradley A Carlson, Soon Jeong Jeong, Rajeev K Shrimali, Dolph
    Hatfield. The selenocysteine tRNA Staf region is essential for
    adequate selenoprotein levels and mouse longevity. Experimental
    biology 2006, San Francisco, CA, April, 2006. Invited Speaker

8.  Bradley A. Carlson, Soon-Jeong Jeong, Rajeev K. Shrimali, Jennifer
    Watts, Gerald F. Combs, Jr.2, Dolph Hatfield. Deletion of the
    Selenocysteine (Sec) tRNA Staf Binding Region Results in Tissue
    Specificity of Sec tRNA and Selenoprotein Expression and Reduces
    Mouse Lifespan. Selenium Meeting 2006, Madison, WI, July, 2006.
    Invited Speaker