Survey Plans

Submittal Form

## Approvals

## 

## 

## 

## 

## 

## \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \_\_\_\_\_\_\_\_\_\_\_\_

George Ison Date

Ship Operations Contract Project Officer, Ship

Safety Officer

## 

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## \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \_\_\_\_\_\_\_\_\_\_\_\_

Glenn Warren Date

Environmental Monitoring and Indicators Team Leader

## \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \_\_\_\_\_\_\_\_\_\_\_\_

Paul Horvatin Date

Monitoring Indicators and Reporting Branch Chief

# IFYLE-2005 

# May Survey Cruise

**Dates** **May 11^th^, 2005 -- May 16^th^, 2005**

**Vessel: Lake Guardian**

#### 

#### [Ports]{.underline} 

**Embark:** Monroe, Michigan: 10:00 EDT May 11^th^ (Turning Basin)

(The Estimated ETA for Lake Guardian in Monroe MI is 7:00 EDT May
11^th^. )

**Disembarkment:** Monroe, Michigan: 16:00 EDT May 16^th^

**[Ports of Call:]{.underline}** None

## Cruise Participants

PI's (indicate project PI if PI will not be on board) status cabin
Citizen

+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr. Greg Boyer SUNY-ESF, Syracuse NY**    | PI  |     | US |
|                                                   |     |     |    |
| glboyer@esf.edu                                   |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) Steven Ragonese                             | T   |     | US |
|                                                   | ech |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) Karen Howard                                | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) Juliette Smith                              | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) Raija Suomela                               | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) **Tracy Meilander (acting coPI for Robert   | s   |     | US |
| Heath)**                                          | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr. Robert Leshkevich**                   | PI  |     | US |
|                                                   |     |     |    |
| George.leshkevich@noaa.gov                        |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) Bradley Robinson                            | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) Chuck Hatt                                  | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) Beth Oswald (for Tom Johengen)              | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr. David Raikow**                        | c   |     | US |
|                                                   | oPI |     |    |
| David.Raikow@noaa.gov                             |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) **Margaret Lansing**                        | Ad  |     | US |
|                                                   | min |     |    |
| > margaret.lansing@noaa.gov                       |     |     |    |
+---------------------------------------------------+-----+-----+----+

[Other pertinent contacts and collaborators who will not be on board in
May]{.underline}:

+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr. Tom Johengen**                        | c   |     | US |
|                                                   | oPI |     |    |
| Thomas.Johengen@noaa.gov                          |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr Hunter Carrick**                       | PI  |     | US |
|                                                   |     |     |    |
| hjc11@psu.edu                                     |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(F\) Jessica Moon                                | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) Dr. **Peter Lavrentyev**                    | PI  |     | US |
|                                                   |     |     |    |
| peter3@uakron.edu                                 |     |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) Ken Moats                                   | s   |     | US |
|                                                   | tud |     |    |
|                                                   | ent |     |    |
+---------------------------------------------------+-----+-----+----+
| \(M\) **Dr. Stuart Ludsin**                       | Ad  |     | US |
|                                                   | min |     |    |
| stuart.ludsin@noaa.gov                            |     |     |    |
+---------------------------------------------------+-----+-----+----+

**[Purpose of Cruise:]{.underline}**

To carry out a survey of both harmful algae and dissolved oxygen in Lake
Erie as part of the NOAA International Field Year on Lake Erie. A full
basin survey will be undertaken. The overall goals of the IFYLE 2005
sampling program are to (1) examine the causes and consequences of
low-oxygen events, (2) evaluate how lake physics and food webs affect
fish production and (3) examine the distribution and timing of harmful
algal blooms in Lake Erie. All three objectives are interconnected
through Lake Erie's food webs with the ultimate application of this
research being to develop forecasts of anoxia, harmful algal blooms and
fish production that can aid in the decision making process.

**[Individual Tasks and Projects listed by PI:]{.underline}**

## Boyer:

Collect samples for molecular and cyanobacterial toxin analysis. This
includes collecting a 25L water sample from 1 m depth using a high speed
submersible pump. This pump can be hand deployed. Samples are returned
to the lab and filtered through glass fiber filters for later toxin,
DNA, and pigment analysis. A vertical net tow will be taken using a
hand-held phytoplankton net (Wisconsin type). These samples occur at
every station.

A second project will collect mud samples using a large box corer to
determine the sedimentation of toxins and toxic organisms to the bottom
sediments. This sampling will occur at limited stations in the western
and eastern basins of Lake Erie

## Leshkevich and Johengen: 

Make optical measurements with Satlantic profiling  radiometer and
surface reference.   Once on station, deploy  floating surface reference
into water and let float away from ship.  Then deploy profiling
radiometer using articulated  arm .  Once  in the water,  lower
radiometer using cable positioned around the movable  winch or
capstan positioned in stern  (I  used this method last year on the EPA 
Lake Erie cruise in August  - Capitan  should remember the routine)  Any
shadows from  articulated arm, etc. should be cast back toward the ship
to avoid shadowing the  instruments.  Make three (3) replicate casts per
station.    Water needs to be taken  at or near time of optical
measurements  and processed  for chlorophyll,  dissolved organic carbon
and suspended matter.   Sampling should take about 30 min and must occur
between 10:00 and 15:00 when the satellite is passing overhead. No
measurements will be taken if it is raining.

## Raikov:

I will collect zooplankton and water (i.e. phytoplankton). A 150um net
will be towed to collect zooplankton from the the entire water column.
This sample will then be stored in a bottle with 95% Ethanol. One liter
of water sampled from the epliminion, metalimnion, and hypolimnion will
be composited in a carboy. This water sample will then be returned to
the lab and filtered. I\'m still deciding on the methods, but since you
want this today\... The composited water will be filtered through a 64um
mesh. The particulates will be discarded. The water will then be
filtered onto a 47mm filter of type to be determined using Tom
Johengen\'s Filter apparatus. The filter will then be frozen. The water
will be discarded. I may make changes to the procedures for processing,
but in terms of ship operation I need to make a zoop tow and take water
samples.

## Heath and Meilander:

I will be investigating how labile dissolved organic carbon (LDOC)
influences phosphorus dynamics and community structure in
bacterioplankton at sites of differing trophic state and oxygen
concentration. From 12-16 stations, I will need 10L of epilimnetic water
(from rosette). From one central basin site and one eastern basin site,
discrete water samples will be analyzed from epilimnion, metalimnion,
and hypolimnion. Water for bacterial number and biomass will be
preserved with formalin. Samples for analysis of algal and bacterial
particulate phosphorus and TSP will be filtered shipboard and frozen.
Whole water will be filtered for DNA extraction and frozen. Initial
oxygen concentrations for LDOC bioassay and bacterial respiration will
be determined via Winkler titration. Phosphate uptake and bacterial
production will be determined radiometrically with 33-P and 3H-leucine.
Also, from 1-2 sites, I will need 12L of water for nutrient amendment
experiments, 10L of water for swap experiments, and 4L for testing of
microbial shunt hypothesis (this is all in addition to the standard
10L).

## Johengen: 

Water samples from each station and depth will be processed for TP, TDP,
SRP, NO3, NH4, SiO2. We plan on sampling at 2 depths in the western
basin and three depths in the central and eastern basin. Water depths in
all basins will be 2m from surface and 2m from bottom. In the central
and eastern basin the third depth will be taken at the base of the
thermocline, which will be determined by the CTD cast and Niskin bottles
being fired on the upcast. When a thermocline is not present the third
depth can be omitted or taken at mid-water column depth. For the remote
sensing water analyses, samples will be collected at stations occupied
only during the daylight hours of 9am -3pm EST, and only if not full
clouds (see Leshkevich and Johengen above). Only a single 2 m depth
water sample will be analyzed per station. The water sample will be
processed for CHL (bulk-GFF method), TSM and DOC according to the
protocols below. CHL will be analyzed onboard, the other analytes are
frozen and returned to the lab for analysis.

# SURVEY Plan

## Chemical background information:

## Core:

Acetone, 4 gal for chlorophyll analysis

> 90% acetone solution will be collected in 1 gallon plastic containers
> and removed by PI-Boyer for disposal at SUNY-ESF.

## Boyer:

glutaraldyde in tubes for phytoplankton preservation (10 ml)

Lugols iodine solution for phytoplankton preservation (10ml).

## Heath and Meilander::

33-P as potassium phosphate (1 mCi)

3H- Leucine (1mCi)

> *Liquid radioactive waste will be stored in plastic containers and
> returned to Kent for disposal (after several decay cycles). Solid
> waste will be collected in a special plastic bag and returned to Kent
> for disposal according to our radiation safety protocols, also.*

\% trichloroacetic acid,

formalin

sodium thiosulfate,

MnSO4,

conc sulfuric acid

alkali-iodide-azide,

NH4Cl,

NaHPO4

Nutrient amendments - glucose, glutamate

## Leshkevich and Johengen: 

None

## Raikov:

Colloidal Silica

95% ethanol (maybe)

#### CORE MEASUREMENTS FOR SURVEYS 

+--------------------------+-------------------+-----------------------+
| **                       | **[Responsible    | **[Tec                |
| [Variable]{.underline}** | Gro               | hnique]{.underline}** |
|                          | up]{.underline}** |                       |
+--------------------------+-------------------+-----------------------+
| **Depth profiles** for   | Guardian support  | EBT, Seabird          |
| temp, O~2~,              | staff             | fluorometer           |
| transmissometry,         |                   |                       |
| fluorometry              |                   |                       |
+--------------------------+-------------------+-----------------------+
| Secchi depths            | Guardian support  | Secchi Disk           |
|                          | staff             |                       |
+--------------------------+-------------------+-----------------------+
| Size fractionated        | Boyer group (May  | 0.2, 2.0, 20 um       |
| chlorophyll:             | cruise)           | filters in parallel,  |
|                          |                   | extraction and        |
| (+2m; bottom --2m; mid   |                   | fluorometric measure  |
| depth in central and     |                   | (Welschmeyer)         |
| eastern basins only)     |                   |                       |
+--------------------------+-------------------+-----------------------+
| Bulk Surface Chlorophyll | Boyer group       | Fluorometric          |
| (1m)                     |                   | (Welschmeyer)         |
+--------------------------+-------------------+-----------------------+
| Surface phycocyanin (1m) | Boyer group       | Fluorometric          |
|                          |                   | (Welschmeyer)         |
+--------------------------+-------------------+-----------------------+
| Nutrients (TN, TDP, SRP, | Johengen          |                       |
| SiO~2~, NO~3~, NH~3~)    |                   |                       |
+--------------------------+-------------------+-----------------------+
| Cyanobacterial toxins    | Boyer group       | Submersible pump      |
+--------------------------+-------------------+-----------------------+
| Phytoplankton taxonomy / | Guardian support  | Wisconsin hand net    |
| diversity                | staff (??)        |                       |
+--------------------------+-------------------+-----------------------+

## 

## 

## Equipment to be brought and used on Deck:

##  (equipment marked with an \* will be provided by the individual PI)

1.  \*Submersible pump (Boyer) (110 volt power required)

2.  Rosette sampler (multiple)

3.  Go Flow bottles (Heath, Johengen)

4.  EBT, DO, Cond, pH, Transmissometer

5.  \*Satlantic profiling radiometer (Leshkevich)

6.  \*Profiling radiometer (Leshkevich)

7.  Large Box corer (Boyer)

8.  150 µ zooplankton net (Raikov)

9.  \*63 µ hand phytoplankton net (Wisconsin type) -- (Boyer)

10. Plankton Centrifuge (Raikow)

## Mooring Requirements:

No mooring experiments are currently planned for this cruise.

## Diving Operations:

No diving operations are currently planned for this cruise.

## Special Operating Requirements:

The ship will sample on a 24 hr schedule. Selected experiments
(Satlantic) can only be done during daylight hours when the satellite is
overhead (10:00 -- 15:00). The are no special requirements for sea or
wind conditions. Nothing is planned to be towed between stations. It is
not necessary that the Lake Guardian be anchored at the station. Our
preference is that you drift on to the station and do not use the props
to hold the ship in positon as it stirs up the bottom mud. It is
necessary the ship record the initial and final latitude and longitude
for each station.

# 

# Lab Space Requests (if known):

Boyer: Chemistry Lab near sink

Johengen: Chemistry Lab

Meilander: Primary Productivity Lab

# Sample Sites and Location

![](media/image1.jpeg){width="6.519444444444445in" height="3.0375in"}

  ---- ----------- ---------- ----------- ------- -------- ------- --------- ----------------------- ----------
  \#   Station     latitude   Longitude   Depth   Date     ETA     Station   Tasks                   Transit
       name                                                        Time (hr)                         time
                                                                                                     (hr)^\*^

  0    Monroe      41.90000   -83.35833           11-May   10:00             Depart Monroe           

  1    881         41.96861   -83.20833   5                12:49   2         CTD/rosette 1m pump     0.83
                                                                             Satlantic Zooplankton   

  2    e60         41.89167   -83.19670   9.5              15:24   2         CTD/rosette 1m pump Box 0.47
                                                                             Core                    

  3    973         41.79250   -83.33306   6                17:09   0.75      CTD/rosette 1m pump     0.88

  4    882 Maumee  41.73444   -83.38417   6                18:39   1         CTD/rosette^2e^ 1m pump 0.43
       R                                                                     Zooplankton             

  5    e59         41.72667   -83.15000   10               21:05   1.25      CTD/rosette 1m pump Box 1.12
                                                                             Core                    

  6    580         41.84806   -83.10667                    22:56   1         CTD/rosette^2e^ 1m pump 0.76
                                                                             Box Core                

  7    e61         41.94667   -83.04500   10      12-May   0:11    0.5       CTD/rosette 1m pump     0.66

  8    e91M        41.84083   -82.91670   10.5             2:07    1         CTD/rosette 1m pump Box 0.88
                                                                             Core                    

  9    e92         41.95000   -82.68670   11               4:29    1         CTD/rosette 1m pump Box 1.28
                                                                             Core                    

  10   968         41.74139   -82.73333                    6:21    0.5       CTD/rosette 1m pump     1.27

  11   e58         41.68500   -82.93330   11.5             8:57    1.5       CTD/rosette^2e^ 1m pump 1.02
                                                                             Zooplankton Box Core    

  12   969         41.60806   -82.92444   8                10:31   1         CTD/rosette 1m pump     0.46
                                                                             Satlantic               

  13   496         41.56778   -82.72139   10               13:50   2.25      CTD/rosette^2e^ 1m pump 1.00
                                                                             satlantic Zooplankton   
                                                                             Box Core                

  14   965         41.50139   -82.50028   13               16:07   1         CTD/rosette^2e^ 1m pump 1.13
       **Central                                                             Box Core                
       Basin**                                                                                       

  15   964         41.48389   -82.18194   8                18:44   1         CTD/rosette 1m pump Box 1.53
                                                                             Core                    

  16   962         41.71667   -82.18444   18               21:15   1         CTD/rosette^2e^ 1m pump 1.40
                                                                             Zooplankton             

  17   412         42.09972   -82.18972           13-May   0:24    0.75      CTD/rosette^2e^ 1m pump 2.30

  18   e42         41.96500   -82.04170   22               2:21    0.75      CTD/rosette 1m pump     1.08

  19   e43         41.78833   -81.94500   23               4:51    1.25      CTD/rosette^2e^ 1m pump 1.16
                                                                             Zooplankton             

  20   e73         41.97778   -81.75690   24               7:09    0.75      CTD/rosette^4e,2m,4h^   1.45
                                                                             1m pump                 

  21   e36         41.93500   -81.47830   23               9:51    1.25      CTD/rosette 1m pump     1.36
                                                                             Zooplankton             

  22   e37         42.11000   -81.57500   24               12:22   1.25      CTD/rosette^2e^ 1m pump 1.15
                                                                             Satlantic               

  23   e38         42.28167   -81.67170                    14:51   1.25      CTD/rosette 1m pump     1.13
                                                                             satlantic               

  24   950         42.58694   -81.44250                    17:50   0.75      CTD/rosette^2e^ 1m pump 2.14

  25   e30         42.43000   -81.20500   21               20:12   0.75      CTD/rosette 1m pump     1.48

  26   e31         42.25333   -81.10670   21               22:12   0.75      CTD/rosette 1m pump     1.16

  27   e78M        42.11667   -81.25000   23      14-May   1:21    2         CTD/rosette^5e^ 1m pump 1.07
                                                                             Zooplankton Box Core    

  28   e32         42.08167   -81.01170   22               3:54    1.25      CTD/rosette 1m pump     1.16
                                                                             Zooplankton             

  29   1003        42.28417   -80.64028                    7:25    1.25      CTD/rosette 1m pump     2.16
                                                                             Zooplankton             

  30   e63         42.41667   -79.80000   45               12:55   1.25      CTD/rosette 1m pump     4.11
       **Eastern                                                             Satlantic               
       Basin**                                                                                       

  31   e15m        42.51667   -79.89330   60               15:09   1.25      CTD/rosette^4e,2m,4h^   0.75
                                                                             1m pump satlantic       

  32   1108        42.71667   -80.14139                    18:26   1.5       CTD/rosette 1m pump     1.69
                                                                             zooplankton Box Core    

  33   449         42.76111   -79.98889                    20:21   1         CTD/rosette^2e^ 1m pump 0.78
                                                                             Zooplankton             

  34   E10         42.68000   -79.69170   32               22:41   0.75      CTD/rosette 1m pump     1.51

  35   E09         42.53833   -79.61670   47      15-May   0:57    1.25      CTD/rosette 1m pump Box 0.92
                                                                             Core (?)                

  36   932         42.79194   -79.21056   21               4:31    1         CTD/rosette 1m pump Box 2.47
                                                                             Core                    

  37   931 Buffalo 42.84972   -78.94222   10               6:30    0.5       CTD/rosette 1m pump     1.33

  38   936         42.47417   -79.40750   \<10             10:46   1         CTD/rosette 1m pump     3.17
                                                                             Satlantic               

  39   942         42.26000   -79.83000   \<10             14:20   1         CTD/rosette^3e^ 1m pump 2.40
       **Central                                                             satlantic               
       Basin**                                                                                       

  40   489         42.16639   -80.30028   22               17:19   0.5       CTD/rosette 1m pump     2.33

  41   947         41.99028   -80.64111   13               19:53   0.5       CTD/rosette 1m pump     1.95

  42   956         41.68833   -81.44917   7                0:46    0.5       CTD/rosette 1m pump     4.28

  43   958         41.52500   -81.70780   8       16-May   3:04    0.5       CTD/rosette^3e^ 1m pump 1.58
       Cleveland                                                                                     

  44   311         41.66667   -82.50000   13               7:33    0.5       CTD/rosette 1m pump     3.90

  0    Monroe      41.90000   -83.35833   \-               12:05   0                                 4.35
  ---- ----------- ---------- ----------- ------- -------- ------- --------- ----------------------- ----------

> \* Transit time is estimated assuming 10 knots/ hr

-   CTD/Rosette bottles will be fired at 2m and 2m above bottom in the
    western basin. Bottles will be fired at 2m (epi), 2m above bottom
    (hypo), and mid depth in the eastern and central basin. Superscripts
    indicate when additional bottles are needed at the epi, meta, or
    hypolimnion.

-   1 m pump will be used to fill a 23L carboy off the fantail. We would
    like to do this simultaneously with the Rosette to save time (fill
    time is 2-3 minutes) at all stations. At selected stations
    (Zooplankton stations), we may need to collect an additional 20 L
    carboy at depth \< 8m. This will be done after the Rosette is back
    on board.