Statement of Work: Jason Keller

1\. [Objectives]{.underline}

The contractor will provide Dr. Michael Iadarola with professional
laboratory services. Jason Keller's work will focus on biochemical and
molecular biological investigations of nervous system plasticity, gene
regulation, and ion channel function in primary cultures of nervous
system cells as well as in vivo studies using animal models.

2\. [Approach]{.underline}

The contractor will be involved in implementing state-of-the-art
live-cell microscopic imaging, cell biology and molecular biology
methods for analysis of ion channel function in vitro and induced gene
expression in spinal cord and brain during states of pharmacological,
physiological or pathophysiological activation.

3\. [Methods]{.underline}

The services that will be performed are as follows:

a\) The contractor will use measurements of fluorescence and radioactive
calcium -- 45 uptake in cultured cell and primary cultures of neurons
and glial cells and stably and transiently transfected cell lines. The
contractor will be able to manipulate plasmids and viral vectors as part
of these studies.

b\) The contractor will maintain detailed records of experimental
procedures and results. High quality laboratory notebooks are
maintained, which will be consulted to extract results and raw data for
processing into graphs for research reports and published manuscripts.

c\) Data will be analyzed using appropriate statistical approaches to
demonstrate significant differences resulting from experimental
manipulations. Familiarity with MetaMorph and other imaging software
packages is also essential.

**Personnel, evaluation and schedule**

A. [Personnel]{.underline}

The contractor has training in basic molecular biological methods such
as RNA extraction, cDNA synthesis, polymerase chain reaction (PCR),
cloning of DNA fragments and cDNAs and subcloning into plasmids and
various forms of differential hybridization. The contractor is capable
of performing experiments using live cell imaging techniques and has
familiarity with live cell imaging techniques using flow through
chambers, microscopy, video and CCD camera recording and use of
MetaMorph and other imaging software packages as necessary for data
acquisition and analysis.

Personnel provided under the contract will comply with the rules and
policies of the NIDCR and its staff.

B. [Evaluation]{.underline}

The contractor will be evaluated daily and if the contractor is
considered to be substandard in the judgment of the supervisor, the
contractor will initially receive notice of the problem from the
supervisor and will be required to take corrective measures and to
demonstrate improved performance.

C. [Schedule]{.underline}

The contractor will perform studies for a minimum of 40 hours per week,
Monday through Friday (except any federal holidays). Failure to arrive
on time or to follow the time schedules or instructions as indicated
shall be considered default of the contract.

D. [Reporting Requirements]{.underline}

The contractor shall provide professional services for Neurobiology and
Pain Therapeautics, NIDCR during the period of October 1, 2007 to
September 26, 2008. The contractor will be paid a total of \$50,400.00

**Justification**

Attached is a request for \$50.400.00 to renew the contract services of
Jason Keller. Under this contract, Jason Keller will provide services to
the Neurobiology and Pain Therapeautics, NIDCR. His contract will run
from October 1, 2007 to September 26, 2008.

These are highly specialized technical procedures and Mr. Keller has all
of the technical skills and practical training to execute the procedures
in a high quality conscientious fashion. The procedures require a great
deal of personal attention to detail, cleanliness and knowledge of
sterile tissue culture technique, good skills with microscopic
microsurgery, good skills with computer databases, statistical analyses,
and practical experience with robotic liquid handling devices,
luminometers and various UV and fluorescent plate readers. Without
someone filling this position, the productivity of our laboratory will
be less than optimal. Considering all of his skills, Mr. Keller is the
ideal candidate for this position.

[Task Description]{.underline}

Firm will issue 25 checks on the following days for the following
amounts:

October 12^th^ for \$2,016.00

October 26^th^ for \$2,016.00

November 9^th^ for \$2,016.00

November 23^rd^ for \$2,016.00

December 7^th^ for \$2,016.00

December 21^st^ for \$2,016.00

January 4^th^ for \$2,016.00

January 18^th^ for \$2,016.00

February 1^st^ for \$2,016.00

February 15^th^ for \$2016.00

February 29^th^ for \$2,016.00

March 14^th^ for \$2,016.00

March 28^th^ for \$2,016.00

April 11^th^ for \$2016.00

April 25^th^ for \$2016.00

May 9^th^ for \$2016.00

May 23^rd^ for \$2016.00

June 6^th^ for \$2016.00

June 20^th^ for \$2016.00

July 3^rd^ for \$2016.00

July 18^th^ for \$2016.00

August 15^th^ for \$2016.00

August 29^th^ for \$2016.00

September 12^th^ for \$2016.00

September 26th for \$2016.00

Total = \$50,400.00

Checks will be issued to Jason Keller. A total of \$50,400.00 shall be
paid in 25 payments. The check should be sent via express mail to the
NIDCR, to the attention of Cynthia Williams at the following address.

Cynthia Williams

Administrative Officer

National Institute of Health

National Institute of Dental and Craniofacial Research

30 Convent Drive Room 5A503

Bethesda, MD 20892

301-496-1483

Preparer, Jacinta Mason, NIDCR