# Developmental Validation Papers

Collins PJ, Hennessy LK, Leibelt CS, Roby RK, Reeder DJ, Foxall PA.
Developmental validation of a single-tube amplification of the 13 CODIS
STR loci, D2S1338, D19S433, and amelogenin: the AmpFlSTR **Identifiler**
PCR amplification kit. *J. Forensic Sci.* 2004; 49(6): 1265-1277.

Cotton EA, Allsop RF, Guest JL, Frazier RR, Koumi P, Callow IP, Seager
A, Sparkes RL. Validation of the AMPFlSTR **SGM Plus** system for use in
forensic casework. *Forensic Sci Int* 2000; 112: 151-161.

Daniels DL, Hall AM, Ballantyne J. SWGDAM developmental validation of a
19-locus Y-STR system for forensic casework. *J Forensic Sci* 2004;
49(4):668-683.

Fregeau CJ, Bowen KL, Fourney RM. Validation of highly polymorphic
fluorescent multiplex short tandem repeat systems using two generations
of DNA sequencers. *J Forensic Sci* 1999; 44(1):133-166. \[**ABI 377**\]

Green RL, Roinestad IC, Boland C, Hennessy LK. Developmental validation
of the **Quantifiler** real-time PCR kits for the quantification of
human nuclear DNA samples. *J. Forensic Sci.* 2005; 50(4): 809-825.

Greenspoon SA, Ban JD, Sykes K, Ballard EJ, Edler SS, Baisden M,
Covington BL. Application of the **BioMek 2000** Laboratory Automation
Workstation and the **DNA IQ System** to the extraction of forensic
casework samples. *J Forensic Sci* 2004; 49(1):29-39.

Greenspoon SA, Ban JD, Pablo L, Crouse CA, Kist FG, Tomsey CS, Glessner
AL, Mihalacki LR, Long TM, Heidebrecht BJ, Braunstein CA, Freeman DA,
Soberalski C, Nathan B, Amin AS, Douglas EK, Schumm JW. Validation and
implementation of the PowerPlex 16 BIO System STR multiplex for forensic
casework. *J Forensic Sci* 2004; 49(1):71-80.

Holt CL, Buoncristiani M, Wallin JM, Nguyen T, Lazaruk KD, Walsh PS.
TWGDAM validation of **AmpFlSTR PCR amplification kits** for forensic
DNA casework. *J Forensic Sci* 2002; 47(1): 66-96.

Kadash K, Kozlowski BE, Biega LA, Duceman BW. Validation study of the
**TrueAllele automated data review system**. *J Forensic Sci* 2004;
49(4):660-667.

Koumi P, Green HE, Hartley S, Jordan D, Lahec S, Livett RJ, Tsang KW,
Ward DM. Evaluation and validation of the **ABI 3700, ABI 3100, and the
MegaBACE 1000 capillary array electrophoresis instruments** for use with
short tandem repeat microsatellite typing in a forensic environment.
*Electrophoresis* 2004; 25(14):2227-2241.

Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, Tomsey
CS, Zachetti JM, Masibay A, Rabbach DR, Amiott EA, Sprecher CJ.
Validation of a 16-locus fluorescent multiplex system. *J Forensic Sci*
2002; 47(4): 773-785. \[**PowerPlex 16**\]

Krenke BE, Viculis L, Richard ML, Prinz M, Milne SC, Ladd C, Gross AM,
Gornall T, Frappier JRH, Eisenberg AJ, Barna C, Aranda XG, Adamowicz,
Budowle B. Validation of a male-specific, 12-locus fluorescent short
tandem repeat (STR) multiplex. *Forensic Sci Int* 2005; 148: 1-14.
\[**PowerPlex Y kit**\]

Lazaruk K, Walsh PS, Oaks F, Gilbert D, Rosenblum BB, Menchen S,
Scheibler D, Wenz HM, Holt C, Wallin J. Genotyping of forensic short
tandem repeat (STR) systems based on sizing precision in a capillary
electrophoresis instrument. *Electrophoresis* 1998;19(1):86-93. \[**ABI
310**\]

Levedakou EN, Freeman DA, Budzynski MJ, Early BE, Damaso RC, Pollard AM,
Townley AJ, Gombos JL, Lewis JL, Kist FG, Hockensmith ME, Terwilliger
ML, Amiott E, McElfresh KC, Schumm JW, Ulery SR, Konotop F, Sessa TL,
Sailus JS, Crouse CA, Tomsey CS, Ban JD, Nelson MS. Characterization and
validation studies of **PowerPlex 2.1**, a nine-locus short tandem
repeat (STR) multiplex system and penta D monoplex. *J Forensic Sci*
2002; 47(4):757-772.

Micka KA, Amiott EA, Hockenberry TL, Sprecher CJ, Lins AM, Rabbach DR,
Taylor JA, Bacher JW, Glidewell DE, Gibson SD, Crouse CA, Schumm JW.
TWGDAM validation of a nine-locus and a four-locus fluorescent STR
multiplex system. J Forensic Sci 1999; 44(6):1243-1257. \[**PowerPlex
1.1 and FFFL**\]

Mulero, J. J., Chang, C. W., Calandro, L. M., Green, R. L., Li, Y.,
Johnson, C. L., and Hennessy, L. K. (2006) Development and Validation of
the AmpFlSTR **Yfiler** PCR Amplification Kit: A Male Specific, Single
Amplification 17 Y-STR Multiplex System. *J Forensic Sci.* 51(1): 64-75.

Nicklas JA, Buel E. Development of an ***Alu*-based, real-time PCR
method for quantitation of human DNA** in forensic samples. *J Forensic
Sci* 2003;48(5):936-944.

Richard ML, Frappier RH, Newman JC. Developmental validation of a
real-time quantitative PCR assay for automated quantification of human
DNA. *J Forensic Sci* 2003; 48(5):1041-1046. \[**CFS qPCR assay**\]

Wallin JM, Buoncristiani MR, Lazaruk KD, Fildes N, Holt CL, Walsh PS.
TWGDAM validation of the **AmpFISTR Blue** PCR amplification kit for
forensic casework analysis. *J Forensic Sci* 1998; 43(4):854-870.

Wilson MR, DiZinno JA, Polanskey D, Replogle J, Budowle B. Validation of
**mitochondrial DNA sequencing** for forensic casework analysis. *Int J
Legal Med* 1995; 108(2):68-74.

# Internal Validation Papers

Buse EL, Putinier JC, Hong MM, Yap AE, Hartmann JM. Performance
evaluation of two multiplexes used in fluorescent short tandem repeat
DNA analysis. *J Forensic Sci* 2003; 48(2):348-357. \[**Profiler
Plus/COfiler**\]

Fox JC, Cave CA, Schumm JW. Development, characterization, and
validation of a sensitive primate-specific quantification assay for
forensic analysis. *Biotechniques* 2003; 34(2):314-322. \[**Bode
quantitation assay**\]

Frank WE, Llewellyn BE, Fish PA, Riech AK, Marcacci TL, Gandor DW,
Parker D, Carter RR, Thibault SM. Validation of the **AmpFlSTR Profiler
Plus** PCR amplification kit for use in forensic casework. *J Forensic
Sci* 2001; 46(3):642-646.

Fregeau CJ, Bowen KL, Leclair B, Trudel I, Bishop L, Fourney RM.
AmpFlSTR **Profiler Plus** short tandem repeat DNA analysis of casework
samples, mixture samples, and nonhuman DNA samples amplified under
reduced PCR volume conditions (25 microL). *J Forensic Sci* 2003;
48(5):1014-1034.

Gaines ML, Wojtkiewicz PW, Valentine JA, Brown CL. **Reduced volume
PCR** amplification reactions using the AmpFlSTR **Profiler Plus** kit.
*J Forensic Sci* 2002; 47(6):1224-1237.

Gill P, Koumi P, Allen H. Sizing short tandem repeat alleles in
capillary array gel electrophoresis instruments*. Electrophoresis*
2001;22:2670-2678. \[**ABI 3700**\]

Greenspoon SA, Lytle PJ, Turek SA, Rolands JM, Scarpetta MA, Carr CD.
Validation of the **PowerPlex 1.1** loci for use in human
identification. *J Forensic Sci* 2000; 45(3):677-683.

Holland MM, Parsons TJ. **Mitochondrial DNA** sequence analysis -
validation and use for forensic casework. *Forensic Sci Rev* 1999;
11(1):22-50.

Isenberg AR, Allen RO, Keys KM, Smerick JB, Budowle B, McCord BR.
Analysis of two multiplexed short tandem repeat systems using capillary
electrophoresis with multiwavelength florescence detection.
*Electrophoresis* 1998;19:94-100.

Johnson CL, Warren JH, Giles RC, Staub RW. Validation and uses of **a
Y-chromosome STR 10-plex** for forensic and paternity laboratories. *J
Forensic Sci* 2003; 48(6):1260-1268.

Kimpton CP, Fisher D, Watson S, Adams M, Urquhart A, Lygo J, Gill P.
Evaluation of an automated DNA profiling system employing multiplex
amplification of four tetrameric STR loci. *Int J Leg Med* 1994;
106:302-311.

Kimpton CP, Oldroyd NJ, Watson SK, Frazier RR, Johnson PE, Millican ES,
Urquhart A, Sparkes BL, Gill P. Validation of highly discriminating
multiplex short tandem repeat amplification systems for individual
identification. *Electrophoresis* 1996; 17(8):1283-1293.

Kline MC, Duewer DL, Newall P, Redman JW, Reeder DJ, Richard M.
Interlaboratory evaluation of short tandem repeat triplex **CTT**\*. *J
Forensic Sci* 1997; 42(5):897-906.

LaFountain MJ, Schwartz MB, Svete PA, Walkinshaw MA, Buel E. TWGDAM
validation of the AmpFlSTR **Profiler Plus** and AmpFlSTR **COfiler**
STR multiplex systems using capillary electrophoresis. *J Forensic Sci*
2001; 46(5):1191-1198.

LaFountain M, Schwartz M, Cormier J, Buel E. Validation of capillary
electrophoresis for analysis of the X-Y homologous **amelogenin** gene.
*J Forensic Sci* 1998; 43(6):1188-1194.

Leibelt C, Budowle B, Collins P, Daoudi Y, Moretti T, Nunn G, Reeder D,
Roby R. Identification of a D8S1179 primer binding site mutation and the
validation of a primer designed to recover null alleles. *Forensic Sci
Int* 2003; 133(3):220-227.

Lygo JE, Johnson PE, Holdaway DJ, Woodroffe S, Whitaker JP, Clayton TM,
Kimpton CP, Gill P. The validation of short tandem repeat (STR) loci for
use in forensic casework. *Int J Legal Med* 1994; 107(2):77-89.

Mandrekar MN, Erickson AM, Kopp K, Krenke BE, Mandrekar PV, Nelson R,
Peterson K, Shultz J, Tereba A, Westphal N. Development of a human DNA
quantitation system. *Croat Med J* 2001; 42(3):336-339. \[**AluQuant**\]

Micka KA, Sprecher CJ, Lins AM, Comey CT, Koons BW, Crouse C, Endean D,
Pirelli K, Lee SB, Duda N, Ma M, Schumm JW. Validation of multiplex
polymorphic STR amplification sets developed for personal identification
applications. *J Forensic Sci* 1996; 41(4):582-590.

Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Brown AL, Budowle B.
Validation of STR typing by capillary electrophoresis. *J Forensic Sci*
2001; 46(3):661-676.

Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B.
Validation of short tandem repeats (STRs) for forensic usage:
performance testing of fluorescent multiplex STR systems and analysis of
authentic and simulated forensic samples. *J Forensic Sci* 2001;
46(3):647-660.

Morley JM, Bark JE, Evans CE, Perry JG, Hewitt CA, Tully G. Validation
of mitochondrial DNA minisequencing for forensic casework. *Int J Legal
Med* 1999; 112(4):241-248.

Mozer TJ. Requirements for complete validation of an STR product.
*Profiles in DNA* 2001; 4(3):14-15.

Nelson MS, Levedakou EN, Matthews JR, Early BE, Freeman DA, Kuhn CA,
Sprecher CJ, Amin AS, McElfresh KC, Schumm JW. Detection of a
primer-binding site polymorphism for the STR locus D16S539 using the
Powerplex 1.1 system and validation of a degenerate primer to correct
for the polymorphism. *J Forensic Sci* 2002; 47(2):345-349.

Pawlowski R, Maciejewska A. Forensic validation of a multiplex
containing nine STRs\--population genetics in northern Poland. *Int J
Legal Med* 2000; 114(1-2):45-49.

Pestoni C, Lareu MV, Rodriguez MS, Munoz I, Barros F, Carracedo A. The
use of the STRs HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS, HUMLPL in
forensic application: validation studies and population data for Galicia
(NW Spain). *Int J Legal Med* 1995; 107(6):283-290.

Potter T. Co-amplification of ENFSI-loci D3S1358, D8S1179 and D18S51:
validation of new primer sequences and allelic distribution among 2874
individuals. *Forensic Sci Int* 2003; 138(1-3):104-110.

Prinz M, Ishii A, Coleman A, Baum HJ, Shaler RC. Validation and casework
application of a Y chromosome specific STR multiplex. *Forensic Sci Int*
2001; 120(3):177-188.

Promega Corporation (2001) Validation of STR Systems Reference Manual.

<http://www.promega.com/techserv/apps/hmnid/referenceinformation/powerplex/ValidationManual.pdf>

Ryan JH, Barrus JK, Budowle B, Shannon CM, Thompson VW, Ward BE. The
application of an automated allele concordance analysis system
(**CompareCalls**) to ensure the accuracy of single-source STR DNA
profiles. *J Forensic Sci* 2004; 49(3):492-499.

Sgueglia JB, Geiger S, Davis J. Precision studies using the **ABI Prism
3100 genetic analyzer** for forensic DNA analysis. *Anal Bioanal Chem*
2003; 376(8):1247-1254.

Shewale JG, Nasir H, Schneida E, Gross AM, Budowle B, Sinha SK.
Y-chromosome STR system, **Y-PLEX 12**, for forensic casework:
development and validation. *J Forensic Sci.* 2004; 49(6): 1278-1290.

Sinha SK, Budowle B, Arcot SS, Richey SL, Chakrabor R, Jones MD,
Wojtkiewicz PW, Schoenbauer DA, Gross AM, Sinha SK, Shewale JG.
Development and validation of a multiplexed Y-chromosome STR genotyping
system, **Y-PLEX 6**, for forensic casework. *J Forensic Sci* 2003;
48(1):93-103.

Sinha SK, Nasir H, Gross AM, Budowle B, Shewale JG. Development and
validation of the **Y-PLEX 5**, a Y-chromosome STR genotyping system,
for forensic casework. *J Forensic Sci* 2003; 48(5):985-1000.

Sparkes R, Kimpton CP, Watson S, Oldroyd NJ, Clayton TM, Barnett L,
Arnold J, Thompson C, Hale R, Chapman J, Urquhart A, Gill P. The
validation of a 7-locus multiplex STR test for use in forensic casework.
(I). Mixtures, ageing, degradation and species studies. *Int J Legal
Med* 1996; 109(4):186-194. \[**FSS SGM assay**\]

Sparkes R, Kimpton C, Gilbard S, Carne P, Andersen J, Oldroyd N, Thomas
D, Urquhart A, Gill P. The validation of a 7-locus multiplex STR test
for use in forensic casework. (II), Artefacts, casework studies and
success rates. *Int J Legal Med* 1996; 109(4):195-204. \[**FSS SGM
assay**\]