+------------------------+---------------------------------------------+
| ![](media/image1.j     | Module 11                                   |
| peg){width="1.90625in" |                                             |
| height="               | **Use and Care of Equipment At the HIV      |
| 1.8729166666666666in"} | Rapid Testing Site**                        |
+------------------------+---------------------------------------------+

+----------------+-----------------------------------------------------+
|                |                                                     |
+----------------+-----------------------------------------------------+
| **Purpose**    | To help you understand exactly what is expected of  |
|                | you concerning the care and use of equipment at the |
|                | HIV rapid test site and to equip you with the       |
|                | necessary skills                                    |
+----------------+-----------------------------------------------------+
| **P            | Module 5: Assuring the Quality of HIV Rapid Testing |
| re-requisite** |                                                     |
|                |                                                     |
| **Modules**    |                                                     |
+----------------+-----------------------------------------------------+
| **Learning     | At the end of this module, you will be able to:     |
| Objectives**   |                                                     |
|                | -   Specify their responsibilities related to       |
|                |     equipment at the HIV rapid test site            |
|                |                                                     |
|                | -   Routinely monitor the temperatures of           |
|                |     refrigerators or freezers                       |
|                |                                                     |
|                | -   Confirm auto pipettes deliver specified volumes |
|                |                                                     |
|                | -   Properly use and maintain centrifuges           |
+----------------+-----------------------------------------------------+
| **Content      | > Rationale for using properly maintained equipment |
| Outline**      | >                                                   |
|                | > Your responsibilities for equipment               |
|                | >                                                   |
|                | > Use and care of equipment at the HIV rapid        |
|                | > testing site                                      |
|                | >                                                   |
|                | > \- Refrigerator and Freezer                       |
|                | >                                                   |
|                | > \- Pipette                                        |
|                | >                                                   |
|                | > \- Centrifuge                                     |
+----------------+-----------------------------------------------------+
| **Handouts**   | Daily Temperature Check Chart                       |
|                |                                                     |
|                | Generic Maintenance Form                            |
+----------------+-----------------------------------------------------+
| ![](media      | Modify this module based on the equipment used at   |
| /image2.wmf){w | the test sites and participants' jobs and           |
| idth="0.491666 | responsibilities.                                   |
| 66666666664in" |                                                     |
| height="0.     |                                                     |
| 44166666666666 |                                                     |
| 665in"}**Notes |                                                     |
| on             |                                                     |
| C              |                                                     |
| ustomization** |                                                     |
+----------------+-----------------------------------------------------+

+-----------------+--------------------------+--------------------------+
| **The Lab       | Equipment is a component |                          |
| Quality         | of the Lab Quality       |                          |
| System**        | System. This component   |                          |
|                 | addresses:               |                          |
|                 |                          |                          |
|                 | -   Equipment selection  |                          |
|                 |                          |                          |
|                 | -   Equipment            |                          |
|                 |     acquisition          |                          |
|                 |                          |                          |
|                 | -   Equipment            |                          |
|                 |     installation and     |                          |
|                 |     initial              |                          |
|                 |                          |                          |
|                 |   calibration/validation |                          |
|                 |                          |                          |
|                 | -   Maintenance service  |                          |
|                 |     and repair           |                          |
|                 |                          |                          |
|                 | -   Troubleshooting      |                          |
|                 |                          |                          |
|                 | -   Retiring equipment   |                          |
|                 |     and disposition      |                          |
+-----------------+--------------------------+--------------------------+
| **Functioning   |                          |                          |
| Equipment is    |                          |                          |
| Vital to        |                          |                          |
| Quality         |                          |                          |
| Service**       |                          |                          |
+-----------------+--------------------------+--------------------------+
|                 | Why is it important to   |                          |
|                 | properly maintain        |                          |
|                 | equipment? Reliable      |                          |
|                 | results aid clinical     |                          |
|                 | diagnosis. Unreliable    |                          |
|                 | results may result in    |                          |
|                 | incorrect diagnosis and  |                          |
|                 | treatment of the         |                          |
|                 | patient. If equipment is |                          |
|                 | properly maintained, it  |                          |
|                 | is less likely to        |                          |
|                 | breakdown before its     |                          |
|                 | next service and is less |                          |
|                 | likely to perform        |                          |
|                 | inadequately due to lack |                          |
|                 | of maintenance.          |                          |
+-----------------+--------------------------+--------------------------+
| **Equipment at  | HIV Rapid Testing can be |                          |
| HIV Rapid       | performed with minimal   |                          |
| Testing Site**  | equipment. At a minimum, |                          |
|                 | there are 4 pieces of    |                          |
|                 | equipment that may be    |                          |
|                 | used at the rapid        |                          |
|                 | testing site.            |                          |
|                 |                          |                          |
|                 | -   Refrigerators store  |                          |
|                 |     reagents, kits, and  |                          |
|                 |     quality control      |                          |
|                 |     materials requiring  |                          |
|                 |     refrigerator storage |                          |
|                 |     (such as Capillus).  |                          |
|                 |                          |                          |
|                 | -   Freezers store       |                          |
|                 |     specimens collected  |                          |
|                 |     for EQA purposes     |                          |
|                 |     prior to transport   |                          |
|                 |     to a reference       |                          |
|                 |     laboratory. Quality  |                          |
|                 |     control specimens    |                          |
|                 |     may also be stored   |                          |
|                 |     in a freezer.        |                          |
|                 |                          |                          |
|                 | -   Pipettes collect or  |                          |
|                 |     transfer specimen to |                          |
|                 |     test device.         |                          |
|                 |                          |                          |
|                 | -   Centrifuges separate |                          |
|                 |     cells from           |                          |
|                 |     serum/plasma         |                          |
|                 |                          |                          |
|                 | All equipment used at    |                          |
|                 | the testing site must be |                          |
|                 | properly maintained.     |                          |
|                 | Using equipment that has |                          |
|                 | not been properly        |                          |
|                 | maintained may           |                          |
|                 | compromise the quality   |                          |
|                 | of test results.         |                          |
+-----------------+--------------------------+--------------------------+
| **Management    | Lab management is        |                          |
| Re              | responsible for making   |                          |
| sponsibilities: | sure the test site is    |                          |
| Ensure Test     | ready to receive and     |                          |
| Site            | install a new piece of   |                          |
| Readiness**     | equipment. This          |                          |
|                 | includes:                |                          |
|                 |                          |                          |
|                 | -   Assigning            |                          |
|                 |     responsibilities for |                          |
|                 |     oversight of all lab |                          |
|                 |     equipment.           |                          |
|                 |                          |                          |
|                 | -   Establishing         |                          |
|                 |     inventory record --  |                          |
|                 |     Each piece of        |                          |
|                 |     equipment must have  |                          |
|                 |     an inventory record. |                          |
|                 |     This record contains |                          |
|                 |     pertinent            |                          |
|                 |     information such as  |                          |
|                 |     manufacturer and     |                          |
|                 |     name of equipment,   |                          |
|                 |     maintenance and      |                          |
|                 |     service record, and  |                          |
|                 |     manufacturer contact |                          |
|                 |     information.         |                          |
|                 |                          |                          |
|                 | -   Establishing         |                          |
|                 |     maintenance program, |                          |
|                 |     including routine    |                          |
|                 |     function checks,     |                          |
|                 |     trouble-shooting,    |                          |
|                 |     and maintenance log  |                          |
|                 |                          |                          |
|                 | -   Developing and       |                          |
|                 |     implementing written |                          |
|                 |     protocols for        |                          |
|                 |     operating procedures |                          |
|                 |                          |                          |
|                 | -   Training the         |                          |
|                 |     operators --         |                          |
|                 |     Everyone using any   |                          |
|                 |     piece of laboratory  |                          |
|                 |     equipment must be    |                          |
|                 |     properly trained.    |                          |
|                 |     Training must        |                          |
|                 |     include              |                          |
|                 |     troubleshooting.     |                          |
+-----------------+--------------------------+--------------------------+
| **Your          | Once a piece of          |                          |
| Re              | equipment is installed   |                          |
| sponsibilities: | at your site, your job   |                          |
| Execute at Test | is to:                   |                          |
| Site**          |                          |                          |
|                 | -   Follow written       |                          |
|                 |     operational          |                          |
|                 |     procedures           |                          |
|                 |                          |                          |
|                 | ```{=html}               |                          |
|                 | <!-- -->                 |                          |
|                 | ```                      |                          |
|                 | -   Conduct routine      |                          |
|                 |     maintenance,         |                          |
|                 |     including function   |                          |
|                 |     checks               |                          |
|                 |                          |                          |
|                 | -   Take corrective      |                          |
|                 |     actions              |                          |
|                 |                          |                          |
|                 | -   Keep records         |                          |
|                 |                          |                          |
|                 | Do not use               |                          |
|                 | malfunctioning           |                          |
|                 | equipment.               |                          |
+-----------------+--------------------------+--------------------------+
| **Function**    | Function checks are      |                          |
| **Checks Verify | activities performed     |                          |
| that Equipment  | periodically to ensure   |                          |
| is Working      | that equipment is        |                          |
| Properly**      | working properly before  |                          |
|                 | use and appropriately    |                          |
|                 | maintained for peek      |                          |
|                 | performance.             |                          |
|                 |                          |                          |
|                 | Function checks should   |                          |
|                 | be performed routinely   |                          |
|                 | such as daily, weekly,   |                          |
|                 | monthly, and after       |                          |
|                 | adjustment or repair.    |                          |
|                 | Examples of function     |                          |
|                 | checks include           |                          |
|                 | monitoring refrigerator  |                          |
|                 | temperatures, verifying  |                          |
|                 | pipette accuracy and     |                          |
|                 | checking centrifuge      |                          |
|                 | speed.                   |                          |
+-----------------+--------------------------+--------------------------+
| **Refrigerator  | What you need to do:     |                          |
| and Freezer:    |                          |                          |
| Use and Care**  | -   Keep organized       |                          |
|                 |                          |                          |
|                 | ```{=html}               |                          |
|                 | <!-- -->                 |                          |
|                 | ```                      |                          |
|                 | -   Periodically clean   |                          |
|                 |     inside and outside   |                          |
|                 |                          |                          |
|                 | -   Ensure door is       |                          |
|                 |     completed sealed     |                          |
|                 |     when closing         |                          |
|                 |                          |                          |
|                 | CAUTION! -- DO NOT store |                          |
|                 | food items or beverages  |                          |
|                 | in laboratory            |                          |
|                 | refrigerator or freezer  |                          |
+-----------------+--------------------------+--------------------------+
| **Refrigerator  | Monitor your             | !                        |
| & Freezer:      | refrigerator and freezer | [](media/image6.png){wid |
| Temperature     | daily and make sure they | th="2.095833333333333in" |
| Checks**        | are within the           | height="1.65in"}         |
|                 | acceptable temperature   |                          |
|                 | ranges:                  |                          |
|                 |                          |                          |
|                 | -   Refrigerator: 2^o^C  |                          |
|                 |     to 8^o^C             |                          |
|                 |                          |                          |
|                 | -   Freezer: -20^o^C,    |                          |
|                 |     -40^o^C, or -80^o^Cs |                          |
+-----------------+--------------------------+--------------------------+
|                 | This photo illustrates   |                          |
|                 | routine monitoring of    |                          |
|                 | temperatures of this     |                          |
|                 | refrigerator. It is a    |                          |
|                 | good practice to attach  |                          |
|                 | the form for recording   |                          |
|                 | temperatures directly on |                          |
|                 | the front of the         |                          |
|                 | refrigerator for easy    |                          |
|                 | access. Inserting it     |                          |
|                 | into a protector page    |                          |
|                 | will guard against       |                          |
|                 | tearing of paper.        |                          |
+-----------------+--------------------------+--------------------------+
| **Refrigerator  | At the end of this       |                          |
| & Freezer:      | module you will find a   |                          |
| Temperature     | sample temperature log   |                          |
| Log**           | for your reference.      |                          |
+-----------------+--------------------------+--------------------------+
| **Types of      | ![                       | ![]                      |
| Pipettes**      | ](media/image7.png){widt | (media/image8.jpeg){widt |
|                 | h="1.9944444444444445in" | h="1.9194444444444445in" |
|                 | height                   | height                   |
|                 | ="0.5180555555555556in"} | ="0.5013888888888889in"} |
|                 |                          |                          |
|                 | **Precision Pipette**    | **Graduated plastic bulb |
|                 |                          | pipette**                |
+-----------------+--------------------------+--------------------------+
|                 | There are two types of   |                          |
|                 | pipettes: precision      |                          |
|                 | pipettes and graduated   |                          |
|                 | plastic bulb pipettes.   |                          |
|                 |                          |                          |
|                 | Precision pipettes       |                          |
|                 | dispense precise and     |                          |
|                 | accurate volumes (e.g.,  |                          |
|                 | 50 µl for Determine).    |                          |
|                 | They are not disposable, |                          |
|                 | but use disposable,      |                          |
|                 | single-use, pipette      |                          |
|                 | tips.                    |                          |
|                 |                          |                          |
|                 | Graduated plastic bulb   |                          |
|                 | pipettes, on the other   |                          |
|                 | hand, dispense           |                          |
|                 | approximate volume, are  |                          |
|                 | easy to use, and are     |                          |
|                 | disposable.              |                          |
|                 |                          |                          |
|                 | Remember, never re-use   |                          |
|                 | disposable items. Doing  |                          |
|                 | so will cause cross      |                          |
|                 | contamination.           |                          |
+-----------------+--------------------------+--------------------------+
| **Pipette: Use  | What you need to do:     |                          |
| and Care**      |                          |                          |
|                 | -   Select the           |                          |
|                 |     appropriate pipette  |                          |
|                 |     for the volume       |                          |
|                 |     required (for        |                          |
|                 |     example, if 50 micro |                          |
|                 |     liters (µl) of       |                          |
|                 |     specimen is          |                          |
|                 |     required, use a 100  |                          |
|                 |     µl pipette)          |                          |
|                 |                          |                          |
|                 | -   Ensure that the      |                          |
|                 |     pipettor, tips, and  |                          |
|                 |     specimen are at the  |                          |
|                 |     same temperature     |                          |
|                 |                          |                          |
|                 | -   Firmly attach tip    |                          |
|                 |                          |                          |
|                 | -   Hold the pipette     |                          |
|                 |     vertically when      |                          |
|                 |     aspirating --        |                          |
|                 |                          |                          |
|                 | -   Place tip just below |                          |
|                 |     the sample           |                          |
|                 |                          |                          |
|                 | -   Avoid air bubbles    |                          |
|                 |                          |                          |
|                 | -   Discard contaminated |                          |
|                 |     tips in appropriate  |                          |
|                 |     container after      |                          |
|                 |     completion of task   |                          |
+-----------------+--------------------------+--------------------------+
|                 | [Never lay the pipette   |                          |
|                 | on its side when liquid  |                          |
|                 | is in the                |                          |
|                 | tip]{.underline} --      |                          |
|                 | doing so will cause the  |                          |
|                 | specimen to flow into to |                          |
|                 | the pipette shaft and    |                          |
|                 | damage the pipette.      |                          |
|                 |                          |                          |
|                 | Air bubbles in the tip   |                          |
|                 | can greatly reduce       |                          |
|                 | pipetting accuracy. If   |                          |
|                 | an air bubble is trapped |                          |
|                 | within the tip during    |                          |
|                 | intake, do the           |                          |
|                 | following:               |                          |
|                 |                          |                          |
|                 | -   Dispense the sample  |                          |
|                 |     into the original    |                          |
|                 |     vessel               |                          |
|                 |                          |                          |
|                 | -   Check the tip        |                          |
|                 |     immersion depth      |                          |
|                 |                          |                          |
|                 | -   Pipette more slowly  |                          |
|                 |                          |                          |
|                 | -   If an air bubble     |                          |
|                 |     appears a second     |                          |
|                 |     time, discard the    |                          |
|                 |     tip and use a new    |                          |
|                 |     one.                 |                          |
|                 |                          |                          |
|                 | Remember these safety    |                          |
|                 | practices:               |                          |
|                 |                          |                          |
|                 | -   Carefully discard    |                          |
|                 |     pipette tips in the  |                          |
|                 |     appropriate          |                          |
|                 |     container. Used      |                          |
|                 |     pipette tips should  |                          |
|                 |     not be found on the  |                          |
|                 |     floor, as this poses |                          |
|                 |     a safety hazard.     |                          |
|                 |                          |                          |
|                 | -   Never re-use a       |                          |
|                 |     pipette tip, which   |                          |
|                 |     causes cross         |                          |
|                 |     contamination and    |                          |
|                 |     will compromise      |                          |
|                 |     patient results. A   |                          |
|                 |     fresh tip should be  |                          |
|                 |     used for each        |                          |
|                 |     sample.              |                          |
+-----------------+--------------------------+--------------------------+
| **Precision     | Precision pipettes       |                          |
| Pipettes        | should be checked        |                          |
| Require         | periodically for         |                          |
| Performance     | performance. You will    |                          |
| Checks**        | need the following       |                          |
|                 | supplies: pipette,       |                          |
|                 | pipette tips, analytical |                          |
|                 | balance, weigh boats,    |                          |
|                 | and distilled or         |                          |
|                 | deionzed water. The      |                          |
|                 | analytical balance       |                          |
|                 | should have a scale of   |                          |
|                 | 0.1 to 0.0001 mg.        |                          |
+-----------------+--------------------------+--------------------------+
| **Pipette:      | Performance checks       |                          |
| Steps for       | include reproducibility  |                          |
| Checking        | and calibration. The     |                          |
| Re              | procedures for checking  |                          |
| producibility** | reproducibility are      |                          |
|                 | described below:         |                          |
|                 |                          |                          |
|                 | 1.  Pipette a series of  |                          |
|                 |     10 samples into a    |                          |
|                 |     weigh boat on an     |                          |
|                 |     analytical scale     |                          |
|                 |                          |                          |
|                 | 2.  Record weight of     |                          |
|                 |     each sample to       |                          |
|                 |     calculate            |                          |
|                 |     calibration results  |                          |
|                 |                          |                          |
|                 | 3.  Verify calculated    |                          |
|                 |     results are within   |                          |
|                 |     limits               |                          |
|                 |                          |                          |
|                 |   ----------- --------   |                          |
|                 | --------- -------------- |                          |
|                 |   **Range**   **Max/     |                          |
|                 | Min**       **Accuracy** |                          |
|                 |                          |                          |
|                 |   10 µl                  |                          |
|                 |    [+]{.underline}   10% |                          |
|                 |                          |                          |
|                 |       1.0 µl             |                          |
|                 |                          |                          |
|                 |   100 µl                 |                          |
|                 |    [+]{.underline}   10% |                          |
|                 |                          |                          |
|                 |       10.0 µl            |                          |
|                 |                          |                          |
|                 |   200 µl                 |                          |
|                 |    [+]{.underline}   10% |                          |
|                 |                          |                          |
|                 |       20.0 µl            |                          |
|                 |   ----------- --------   |                          |
|                 | --------- -------------- |                          |
|                 |                          |                          |
|                 | 4.  If the results are   |                          |
|                 |     not within limits,   |                          |
|                 |     remove from service  |                          |
|                 |     until appropriate    |                          |
|                 |     adjustment can be    |                          |
|                 |     made                 |                          |
|                 |                          |                          |
|                 | 5.  Decontaminate        |                          |
|                 |     pipette and scale    |                          |
|                 |     after use            |                          |
+-----------------+--------------------------+--------------------------+
| **Pipette:      | See the following        |                          |
| Tr              | troubleshooting chart.   |                          |
| oubleshooting** |                          |                          |
|                 | <table>                  |                          |
|                 | <colgroup>               |                          |
|                 | <col                     |                          |
|                 | style="width: 33%" />    |                          |
|                 | <col                     |                          |
|                 | style="width: 33%" />    |                          |
|                 | <col                     |                          |
|                 | style="width: 33%" />    |                          |
|                 | </colgroup>              |                          |
|                 | <tbody>                  |                          |
|                 | <tr class="odd">         |                          |
|                 | <td><stro                |                          |
|                 | ng>Problem</strong></td> |                          |
|                 | <td><strong>Potential    |                          |
|                 | Cause</strong></td>      |                          |
|                 | <td><str                 |                          |
|                 | ong>Action</strong></td> |                          |
|                 | </tr>                    |                          |
|                 | <tr class="even">        |                          |
|                 | <td>Leakage</td>         |                          |
|                 | <td><p>Tip(s)            |                          |
|                 | incorrectly              |                          |
|                 | attached</p>             |                          |
|                 | <p>Foreign articles      |                          |
|                 | between the tip and      |                          |
|                 | cone</p>                 |                          |
|                 | <p>O-ring                |                          |
|                 | damaged</p></td>         |                          |
|                 | <td><p>Attach            |                          |
|                 | firmly</p>               |                          |
|                 | <p>Clean tip cones</p>   |                          |
|                 | <p>Change the            |                          |
|                 | O-ring</p></td>          |                          |
|                 | </tr>                    |                          |
|                 | <tr class="odd">         |                          |
|                 | <td>Inaccurate           |                          |
|                 | dispensing</td>          |                          |
|                 | <td><p>Incorrect         |                          |
|                 | operation</p>            |                          |
|                 | <p>Tip incorrectly       |                          |
|                 | attached</p></td>        |                          |
|                 | <td><p>Follow            |                          |
|                 | manufacturer’s           |                          |
|                 | instructions             |                          |
|                 | carefully</p>            |                          |
|                 | <p>Firmly attach         |                          |
|                 | tip</p></td>             |                          |
|                 | </tr>                    |                          |
|                 | </tbody>                 |                          |
|                 | </table>                 |                          |
+-----------------+--------------------------+--------------------------+
| **Centrifuge:   | What you need to do:     |                          |
| Use and Care**  |                          |                          |
|                 | -   Always operate with  |                          |
|                 |     the lids closed --   |                          |
|                 |     Operating a          |                          |
|                 |     centrifuge without   |                          |
|                 |     the lid closed poses |                          |
|                 |     an unnecessary       |                          |
|                 |     safety hazard.       |                          |
|                 |                          |                          |
|                 | ```{=html}               |                          |
|                 | <!-- -->                 |                          |
|                 | ```                      |                          |
|                 | -   Balance contents     |                          |
|                 |     before turning on.   |                          |
|                 |     -- For example, if   |                          |
|                 |     there is only one    |                          |
|                 |     sample to be         |                          |
|                 |     centrifuged, a tube  |                          |
|                 |     identical in size    |                          |
|                 |     and volume must be   |                          |
|                 |     placed in the rotor  |                          |
|                 |     opposite the tube.   |                          |
|                 |     Note: The rotor is   |                          |
|                 |     the part of the      |                          |
|                 |     centrifuge that      |                          |
|                 |     holds the tubes and  |                          |
|                 |     rotates during       |                          |
|                 |     operation.           |                          |
|                 |                          |                          |
|                 | -   Check for vibration  |                          |
|                 |     -- There may be      |                          |
|                 |     several reasons why  |                          |
|                 |     a centrifuge         |                          |
|                 |     vibrates. When       |                          |
|                 |     vibration occurs,    |                          |
|                 |     you'll need to:      |                          |
|                 |                          |                          |
|                 |     -   Stop operation   |                          |
|                 |         of the           |                          |
|                 |         centrifuge.      |                          |
|                 |                          |                          |
|                 |     -   Determine the    |                          |
|                 |         cause of the     |                          |
|                 |         noise or         |                          |
|                 |         vibration.       |                          |
|                 |                          |                          |
|                 |     -   Correct          |                          |
|                 |         immediately to   |                          |
|                 |         prevent severe   |                          |
|                 |         damage to the    |                          |
|                 |         centrifuge or    |                          |
|                 |         injury to the    |                          |
|                 |         worker. Refer to |                          |
|                 |         the owner's      |                          |
|                 |         manual for       |                          |
|                 |         possible causes  |                          |
|                 |         aside from       |                          |
|                 |         improper         |                          |
|                 |         balancing        |                          |
|                 |                          |                          |
|                 | -   Do not open the lid  |                          |
|                 |     until the rotor has  |                          |
|                 |     come to a complete   |                          |
|                 |     stop                 |                          |
|                 |                          |                          |
|                 | -   Keep lids on tubes   |                          |
|                 |     when spinning -- Do  |                          |
|                 |     not take the tops    |                          |
|                 |     off the tubes before |                          |
|                 |     spinning. Doing so   |                          |
|                 |     will cause splashing |                          |
|                 |     and creating of      |                          |
|                 |     aerosols from        |                          |
|                 |     potentially          |                          |
|                 |     infectious material  |                          |
+-----------------+--------------------------+--------------------------+
| **Centrifuges:  | Separation activity is a | ![                       |
| Function        | function of both         | ](media/image9.png){widt |
| Checks**        | centrifugal force and    | h="2.1694444444444443in" |
|                 | timing. Proper balance,  | height                   |
|                 | lubrication and rotor    | ="1.6277777777777778in"} |
|                 | function are essential   |                          |
|                 | for proper               |                          |
|                 | centrifugation to occur. |                          |
+-----------------+--------------------------+--------------------------+
| **Centrifuge:   | When cleaning the        |                          |
| Routine         | centrifuge:              |                          |
| Maintenance**   |                          |                          |
|                 | -   Clean interior daily |                          |
|                 |     with soap and water, |                          |
|                 |     wipe with a          |                          |
|                 |     disinfectant         |                          |
|                 |                          |                          |
|                 | -   Wipe spills using    |                          |
|                 |     10% bleach solution  |                          |
|                 |                          |                          |
|                 | -   After cleaning, run  |                          |
|                 |     the centrifuge at    |                          |
|                 |     varying RPMs to      |                          |
|                 |     check the braking    |                          |
|                 |     mechanism and ensure |                          |
|                 |     a smooth gradual     |                          |
|                 |     stop                 |                          |
|                 |                          |                          |
|                 | When noticing unusual    |                          |
|                 | noises or vibrations:    |                          |
|                 |                          |                          |
|                 | -   Stop operation of    |                          |
|                 |     the centrifuge       |                          |
|                 |                          |                          |
|                 | -   Follow               |                          |
|                 |     manufacturer's       |                          |
|                 |     recommendation on    |                          |
|                 |     activation and       |                          |
|                 |     release of brakes    |                          |
|                 |                          |                          |
|                 | -   Correct immediately  |                          |
|                 |     to prevent severe    |                          |
|                 |     damage to the        |                          |
|                 |     centrifuge or injury |                          |
|                 |     to the worker        |                          |
|                 |                          |                          |
|                 | Inspect for evidence of  |                          |
|                 | wear, cracks in fitting, |                          |
|                 | corrosion, uneven wear,  |                          |
|                 | or signs of fatigue by   |                          |
|                 | checking:                |                          |
|                 |                          |                          |
|                 | -   Head, shaft head and |                          |
|                 |     coupling             |                          |
|                 |                          |                          |
|                 | -   Rotor                |                          |
|                 |                          |                          |
|                 | -   Brushes and bearings |                          |
|                 |                          |                          |
|                 | -   Power supply         |                          |
|                 |                          |                          |
|                 | -   Motor and lubricant  |                          |
|                 |                          |                          |
|                 | -   Gaskets, seals,      |                          |
|                 |     mounts and           |                          |
|                 |     lubricants           |                          |
|                 |                          |                          |
|                 | Brushes need to be       |                          |
|                 | inspected every 3-6      |                          |
|                 | months and replaced      |                          |
|                 | according to             |                          |
|                 | manufacturer             |                          |
|                 | specifications.          |                          |
+-----------------+--------------------------+--------------------------+
| **Centrifuge    | Follow these safety      |                          |
| Safety**        | rules when operating a   |                          |
|                 | centrifuge:              |                          |
|                 |                          |                          |
|                 | -   Increase the speed   |                          |
|                 |     slowly until optimal |                          |
|                 |     speed is reached     |                          |
|                 |                          |                          |
|                 | -   Disconnect the       |                          |
|                 |     centrifuge from the  |                          |
|                 |     electrical source    |                          |
|                 |     before preventive    |                          |
|                 |     maintenance,         |                          |
|                 |     cleaning or          |                          |
|                 |     inspection           |                          |
|                 |                          |                          |
|                 | -   Take caution when    |                          |
|                 |     removing spills and  |                          |
|                 |     broken specimen      |                          |
|                 |     tubes after a run    |                          |
|                 |                          |                          |
|                 | -   If tubes are broken, |                          |
|                 |     keep the door closed |                          |
|                 |     and allow to sit     |                          |
|                 |     undisturbed for 30   |                          |
|                 |     minutes before       |                          |
|                 |     attempting to clean  |                          |
|                 |                          |                          |
|                 | -   Use tweezers to      |                          |
|                 |     remove broken glass  |                          |
|                 |                          |                          |
|                 | Simply turning the power |                          |
|                 | off does not remove      |                          |
|                 | power to the centrifuge. |                          |
+-----------------+--------------------------+--------------------------+
| **Keep a Log    | It is a good practice to |                          |
| for All         | keep a log to document   |                          |
| Maintenance     | problems, corrective     |                          |
| Activities**    | action, preventive       |                          |
|                 | maintenance, cleaning,   |                          |
|                 | and inspections. At the  |                          |
|                 | end of the module, you   |                          |
|                 | will find a sample,      |                          |
|                 | generic maintenance log  |                          |
|                 | for your reference.      |                          |
+-----------------+--------------------------+--------------------------+
| **Exercise:     | Use what you have        |                          |
| Create a        | learned in this module   |                          |
| Maintenance     | and create a maintenance |                          |
| Activity Log**  | checklist specific to    |                          |
|                 | your test site.          |                          |
|                 |                          |                          |
|                 | Create a list of         |                          |
|                 | maintenance activities   |                          |
|                 | on a daily, weekly,      |                          |
|                 | monthly, and yearly      |                          |
|                 | basis                    |                          |
+-----------------+--------------------------+--------------------------+

+-----------------+----------------------------------------------------+
| ![](me          | -   Functioning equipment is vital to quality      |
| dia/image10.wmf |     service                                        |
| ){width="1.1701 |                                                    |
| 388888888888in" | -   DO NOT use malfunctioning equipment            |
| heigh           |                                                    |
| t="0.8319444444 | -   DO NOT store food items or beverages in        |
| 444445in"}**Key |     laboratory refrigerator or freezer             |
| message**       |                                                    |
|                 | -   DO NOT re-use pipette tips or graduated        |
|                 |     plastic bulb pipettes                          |
+-----------------+----------------------------------------------------+

+-----------------+----------------------------------------------------+
| ![](me          | Module Review                                      |
| dia/image11.wmf |                                                    |
| ){width="1.0in" |                                                    |
| height="0.96041 |                                                    |
| 66666666667in"} |                                                    |
+=================+====================================================+
| Find out how    |                                                    |
| much you have   |                                                    |
| learned by      |                                                    |
| answering these |                                                    |
| questions.      |                                                    |
+-----------------+----------------------------------------------------+
| Why is it       |                                                    |
| important to    |                                                    |
| keep equipment  |                                                    |
| in optimal      |                                                    |
| condition?      |                                                    |
+-----------------+----------------------------------------------------+
| Describe proper |                                                    |
| use and care    |                                                    |
| for:            |                                                    |
|                 |                                                    |
| \- Refrigerator |                                                    |
| and freezer     |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_\_  |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_ - |                                                    |
| Pipettes        |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_\_  |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_ - |                                                    |
| Centrifuges     |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_    |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
+-----------------+----------------------------------------------------+
| What are some   |                                                    |
| routine         |                                                    |
| maintenance     |                                                    |
| activities      |                                                    |
| performed on:   |                                                    |
|                 |                                                    |
| \- Refrigerator |                                                    |
| and freezer?    |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_\_  |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_ - |                                                    |
| Pipettes?       |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_\_  |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_ - |                                                    |
| Centrifuges?    |                                                    |
|                 |                                                    |
| \_\_\_\_\_\_    |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
+-----------------+----------------------------------------------------+
| Describe your   |                                                    |
| r               |                                                    |
| esponsibilities |                                                    |
| for equipment   |                                                    |
| at the test     |                                                    |
| site.           |                                                    |
|                 |                                                    |
| \_\_\_          |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
| \_\_\_\_\_\_\_\ |                                                    |
| _\_\_\_\_\_\_\_ |                                                    |
+-----------------+----------------------------------------------------+

  -------------------------------- ------------ ---------- -------------- ---------- -------------- -------- --------
  **DAILY TEMPERATURE CHECK CHART                                                                            
  FOR                                                                                                        
  REFRIGERATOR/FREEZER/INCUBATOR                                                                             
  \# Thermometer \# Temp set[:                                                                               
  ]{.underline}**                                                                                            

                                                                                                             

                                                                                                             

                                                                                                             

                                                                                                             

  ** **                            **Month**    ** **                     **Year**   ** **                   

  **Date**                         **Temp                  **Initials**              **Comments**            
                                   Observed**                                                                

  1                                ** **                   ** **                     ** **                   

  2                                ** **                   ** **                     ** **                   

  3                                ** **                   ** **                     ** **                   

  4                                ** **                   ** **                     ** **                   

  5                                ** **                   ** **                     ** **                   

  6                                ** **                   ** **                     ** **                   

  7                                ** **                   ** **                     ** **                   

  8                                ** **                   ** **                     ** **                   

  9                                ** **                   ** **                     ** **                   

  10                               ** **                   ** **                     ** **                   

  11                               ** **                   ** **                     ** **                   

  12                               ** **                   ** **                     ** **                   

  13                               ** **                   ** **                     ** **                   

  14                               ** **                   ** **                     ** **                   

  15                               ** **                   ** **                     ** **                   

  16                               ** **                   ** **                     ** **                   

  17                               ** **                   ** **                     ** **                   

  18                               ** **                   ** **                     ** **                   

  19                               ** **                   ** **                     ** **                   

  20                               ** **                   ** **                     ** **                   

  21                               ** **                   ** **                     ** **                   

  22                               ** **                   ** **                     ** **                   

  23                               ** **                   ** **                     ** **                   

  24                               ** **                   ** **                     ** **                   

  25                               ** **                   ** **                     ** **                   

  26                               ** **                   ** **                     ** **                   

  27                               ** **                   ** **                     ** **                   

  28                               ** **                   ** **                     ** **                   

  29                               ** **                   ** **                     ** **                   

  30                               ** **                   ** **                     ** **                   

  31                               ** **                   ** **                     ** **                   

                                                                                                             

                                                                                                             

                                                           Supervisor:               (Initials)              

                                                           Name:                                             

                                                           Date:                                             

                                                                                                             

                                                           Binder \#                                         

                                                           Storage                                           
                                                           Location                                          

                                                                                                             

                                                                                                             

                                                                                                             

                                                Document                                                     
                                                control                                                      
                                                No.                                                          
  -------------------------------- ------------ ---------- -------------- ---------- -------------- -------- --------

  ------------------------------- ------- ------- ------- ------- ------- ----------------- ------- ------- ------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- --------
  **Generic Maintenance Form**                                                                                                                                                                                                                                                                                   

  Model                                                                   Serial                                                                                                                                                                                                                                 
  no.\_\_\_\_\_\_\_\_\_\_\_\_\_                                           no.\_\_\_\_\_\_                                                                                                                                                                                                                        

  MONTH:                          YEAR:                                                                                                                                                                                                                                                                          

  **Daily Maintenance             **1**   **2**   **3**   **4**   **5**   **6**             **7**   **8**   **9**   **10**   **11**   **12**   **13**   **14**   **15**   **16**   **17**   **18**   **19**   **20**   **21**   **22**   **23**   **24**   **25**   **26**   **27**   **28**   **29**   **30**   **31**
  (Activity)**                                                                                                                                                                                                                                                                                                   

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

  **Weekly Maintenance            **1**   **2**   **3**   **4**   **5**   **6**             **7**   **8**   **9**   **10**   **11**   **12**   **13**   **14**   **15**   **16**   **17**   **18**   **19**   **20**   **21**   **22**   **23**   **24**   **25**   **26**   **27**   **28**   **29**   **30**   **31**
  (Activity)**                                                                                                                                                                                                                                                                                                   

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

  **Monthly Maintenance           **1**   **2**   **3**   **4**   **5**   **6**             **7**   **8**   **9**   **10**   **11**   **12**   **13**   **14**   **15**   **16**   **17**   **18**   **19**   **20**   **21**   **22**   **23**   **24**   **25**   **26**   **27**   **28**   **29**   **30**   **31**
  (Activity)**                                                                                                                                                                                                                                                                                                   

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  

                                                                                                                                                                                                                                                                                                                  
  ------------------------------- ------- ------- ------- ------- ------- ----------------- ------- ------- ------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- -------- --------