1\. Oligonucleotides for plasmid construction

pGSO146 pET21b-hfq (amp^r^)

#731 5'-TAT ACA TAT GGC TAA GGG GCA ATC TTT ACA AG

#680 5'-CCA AGC TTA TTC GGT TTC TTC GCT GTC C

pGSO147 pSP64-T7/oxyS~G102C~ (amp^r^) linearized with HindIII

#819 5'-CTT TTG CGG ATC TCC ACG ATC CGC AAG CTT GTA

#820 5'-TAC [AAG CTT]{.underline} GCG GAT CGT GGA GAT CCG CAA AAG

pGSO136 pSP64- T7/oxyS~64109~ (amp^r^) linearized with HindIII

#691 5\'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA ATA ACT AAA GCC AA

#690 5\'-TAC [AAG CTT]{.underline} GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO137 pSP64- T7/spf (amp^r^) linearized with HindIII

#786 5\'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGT AGG GTA CAG AGG TAA G

#787 5\'-TAC [AAG CTT]{.underline} TAA AAA ACG CCC CAG TC

pGSO138 pSP64- T7/fhlA~-107+96~ (amp^r^) linearized with HindIII

#790 5'-CTT GAA TTC TAA TAC GAC TCA CTA TAG GGA CCA CAC GCC GAA CCG C

#791 5\'-TAC [AAG CTT]{.underline} CTC ACA CAG CGA GGC C

pGSO148 pSP64- T7/fhlA~C-13G~ (amp^r^) linearized with HindIII

#821 5'-GCT TTG CGG TGC TTT CGT GGA AGA ACA AAA TGT

#822 5'-ACA TTT TGT TCT TCC ACG AAA GCA CCG CAA AGC

pGSO115 pCR2.1-T3/6S (amp^r^, kan^r^) linearized with SmaI

T3/6S(5\'-3) 5\'- GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GAT TTC TCT
GAG ATG TTC GC

6S(3\'-3) 5\'- CG[C CCG GG]{.underline}A ATC TCC GAG ATG CCG CC

pGSO113 pCR2.1-T3/5S (amp^r^, kan^r^) linearized with DraI

T3/5S(5\'-1) 5\'-GCC AAG CTC GAA ATT AAC CCT CAC TAA AGG GTG CCT GGC GGC
CGT AGC GCG

5S(3\'-1) 5\'-CCC CGG G[TT TAA A]{.underline}TG CCT GGC AGT TCC CTA CTC)

pGSO139 pCR2.1-T3/oxyS (amp^r^, kan^r^) linearized with HindIII

#797 5\'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#690 5\'-TAC [AAG CTT]{.underline} GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO140 pCR2.1-T3/oxyS~190~ (amp^r^, kan^r^) linearized with DraI

#797 5\'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#800 5\'-GC[T TTA AA]{.underline}A GTT CAC GTT GGC TTT AG

pGSO141 pCR2.1-T3/oxyS~169~ (amp^r^, kan^r^) linearized with SspI

#797 5\'-CGA ATT AAC CCT CAC TAA AGG AAA CGG AGC GGC ACC TC

#799 5\'-GC[A ATA TT]{.underline}A TTC GAG TTG AGA AAC AC

pGSO142 pCR2.1-T3/oxyS~48109~ (amp^r^, kan^r^) linearized with HindIII

#798 5\'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G

#690 5\'-TAC [AAG CTT]{.underline} GCG GAT CCT GGA GAT CCG CAA AAG TT

pGSO143 pCR2.1-T3/oxyS~4890~ (amp^r^, kan^r^) linearized with DraI

#798 5\'-CGA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC G

#800 5\'-GC[T TTA AA]{.underline}A GTT CAC GTT GGC TTT AG

pGSO144 pGEM2-T3/oxyS~4869~ (amp^r^) linearized with SspI

#801 5'-AAT TCA ATT AAC CCT CAC TAA AGG AGA GTT TCT CAA CTC GAA TAA TAT
TA

#802 5'-AGC TT[A ATA TT]{.underline}A TTC GAG TTG AGA AAC TCT CCT TTA
GTG AGG GTT AAT TG

pGSO145 pGEM2-T3/oxyS~6490~ (amp^r^) linearized with DraI

#803 5'-AAT TCA ATT AAC CCT CAC TAA AGG AAT AAC TAA AGC CAA CGT GAA CTT
TTA A

#804 5'-AGC [TTT AAA]{.underline} AGT TCA CGT TGG CTT TAG TTA TTC CTT
TAG TGA GGG TTA ATT G

2\. Oligonucleotides for oligo blocking analysis

anti-OxyS~1-20~ 5'-AAG AGG TGC CGC TCC GTT TC

anti-OxyS~6-27~ 5'-GGG TTA AAA GAG GTG CCG CTC C

anti-OxyS~20-40~ 5'-GCA GTG ACT TCA AGG GTT AA

anti-OxyS~52-71~ 5'-AGT TAT TCG AGT TGA GAA AC

anti-OxyS~64-83~ 5'-CAC GTT GGC TTT AGT TAT TC

anti-OxyS~76-92~ 5'-GCA AAA GTT CAC GTT GG

3\. Oligonucleotides for primer extension assay

OxyS RNA

#188 5'-GCA AAA GTT CAC GTT GG

*fhlA* mRNA

#153 5'-TGT CCG AGA TCA CTC ATC GGT GTA TAT GAC

Spot42 RNA

#823 5'-CAG CCA AAT CCG ATT ACG