![](media/image1.wmf){width="2.6055555555555556in"
height="3.272222222222222in"}

![](media/image4.wmf){width="0.4270833333333333in"
height="0.40902777777777777in"}

**Clarion Inn and Conference Center**

**Shepherdstown, West Virginia**

**21 -- 28 September 2003**

![](media/image6.png){width="6.625in" height="1.0798611111111112in"}

**National Fish Health Research**

**Laboratory**

#### Foreword

Approximately 27 years ago, an agreement was initiated between the
former Soviet Union and the United States of America to increase
bilateral emphasis and collaboration on issues of environmental
conservation and protection. In 1982, this program was amended by the
addition of Project 02.05-81, which specifically concentrated in
"Ichthyology and Aquaculture." The program is administered through the
Russian Federation Inter-Department Ichthyological Commission, the
Russian Academy of Sciences, the Ministry of Agriculture and Foodstuffs
of Russia - Department of Fisheries, and the U.S. Department of the
Interior. Bilateral interactions in the area of fish health/disease have
been principally achieved through collaborations between the Laboratory
of Ichthyopathology of the All Russian Research Institute of Freshwater
Fisheries (ARRIFF; Rybnoe, Russia) and the National Fish Health Research
Laboratory (NFHRL) of the Department of the Interior - United States
Geological Survey (Kearneysville, WV, USA). In 1998, the first bilateral
conference, *Aquaculture and Fish Health*, was hosted by the ARRIFF,
Laboratory of Ichthyopathology in Rybnoe (Russia). The National Fish
Health Research Laboratory is proud to reciprocate the hospitality of
our Russian colleagues and host the second bilateral conference:
*Aquatic and Marine Animal Health 2003* at the Clarion Inn in
Shepherdstown (West Virginia).

Each of our delegates was specifically invited based on their scientific
contributions to the field of marine and aquatic animal health. We are
indeed honored to host this distinguished group of scientists. You will
each have the opportunity to review your individual area of expertise
and collectively deliberate upon issues associated with feral,
commercial, and aquaculture populations of aquatic and marine animals of
importance to Russia and the United States. Topics will include
presentations on the epidemiology, diagnosis, control, treatment,
prevention and management of pathogens, as well as environmental and
toxicological parameters cultured and feral aquatic and marine animal
health. Further reviews will address mitigation and restoration
fisheries, endangered species, and invasive or non-indigenous species.
Abstracts for the conference will be published in a proceedings book and
review articles for each of the individual presentations will be
published in a peer-reviewed, special-issue of Aquaculture/Annual Review
of Fish Diseases. It is our sincere hope that the time we spend together
this week will create new friendships and inspire future collaborative
research efforts between our countries.

######### Rocco C. Cipriano Igor S. Shchelkunov

Department of the Interior, U.S.G.S. All Russian Research Institute of
Freshwater National Fish Health Research Laboratory Fisheries -
Laboratory of Ichthyopathology

American co-chairman Russian co-chairman

![](media/image7.png){width="1.75in" height="0.6458333333333334in"}

# 

# [An Open Letter to Delegates]{.underline}

It is with great pleasure and pride that I welcome each of you to
Leetown, West Virginia and the National Fish Health Research Laboratory.
As delegates to the *Second United States and Russia Bilateral Symposium
on Fish Health and the Environment* you each bring unique scientific
credentials and experiences to share with colleagues from the United
States and Russia. Over the course of the next week you will each have
the opportunity to present the results of your investigations in various
aspects of fish and aquatic animal health, to share your experiences and
wisdom. But perhaps more importantly, we all have the opportunity to
learn from one another and to take back to our respective institutions
new insights in our approaches to fish and aquatic animal health.

It is also my hope that over the course of this conference that you will
develop new friendships and professional relationships that will
continue well beyond the short time that we have together here in West
Virginia. It is the development of professional associations and our
collective abilities to collaborate on future fish and aquatic animal
health issues and research that will be the lasting testament to the
success of this conference. This is the driving force behind our
long-standing relationship with the Laboratory of Ichthyopathology of
the All Russian Research Institute of Freshwater Fisheries (ARRIFF;
Rybnoe, Russia). It is in this regard that I personally thank Dr. Alexey
M. Bagrov of the All Russian Research Institute of Freshwater Fisheries
and Sergey I. Nikonorov of the Interagency Ichthyological Commission
(Moscow) for their support and cooperation in organizing this bilateral
conference.

The conference could also not have been possible without the generous
support of the Khaled Bin Sultan, Living Oceans Foundation. Serving as
the principal sponsor, the Foundation provided the logistical and travel
support for the delegates and will support the publication of articles
from the conference in a special-issue of Aquaculture/Annual Review of
Fish Diseases. I truly expect that the publication will become a classic
example of successful international cooperation in fish and aquatic
animal health research.

On behalf of the staff of the National Fish Health Research Laboratory I
want to wish you success in your conference proceedings and hope that
you enjoy your stay in West Virginia.

**Frank M. Panek**, Ph.D.

Scientific Director

National Fish Health Research Laboratory

**[Organizing Committee:]{.underline}**

**Dr. Rocco C. Cipriano**, co-chairman, Senior Research Microbiologist,
United States Geological Survey, National Fish Health Research
Laboratory, 11700 Leetown Road, Kearneysville, WV 25430

**Dr. Igor S. Shchelkunov**, co-chairman, Senior Researcher - All
Russian Research Institute of Freshwater Fisheries, Laboratory of
Ichthyopathology, Rybnoe, Dmitrov Region, Moscow Oblast, 141821 Russia

**Dr. Mohamed Faisal**, Professor, Department of Pathobiology and
Diagnostic Investigations, Michigan State University, College of
Veterinary Medicine, A-532 East Fee, Hall, East Lansing, MI 48824

**Dr. Frank M. Panek**, Scientific Director - United States Geological
Survey, National Fish Health Research Laboratory, 11700 Leetown Road,
Kearneysville, WV 25430

**Sergey I. Nikonorov**, Deputy Executive Director, Interagency
Ichthyological Commission, Ulitsa Tverskaya 27, Moscow, 125009, Russia

**[Acknowledgments:]{.underline}**

![](media/image8.png){width="0.7409722222222223in" height="0.875in"}

![](media/image10.jpeg){width="1.0in" height="0.8270833333333333in"}

The members of the organizing committee, as well as the conference
delegates, are especially grateful to the Khaled Bin Sultan Living
Oceans for serving as the principal financial sponsor of this
conference. We are also grateful to the United States Fish and Wildlife
Service Office of International Affairs for funding the airfare travel
of some of the Russian delegates. This conference would not have been
possible without the profound financial sponsorship of these two
organizations. We deeply appreciate the administrative assistance of Dr.
Mohamed Faisal (Director) and Mrs. Sharon Burrell (Administrative
Assistant) of the Khaled Bin Sultan Living Oceans Foundation for their
personal assistance with the travel arrangements of the conference
delegates and their contributions to the development of the scientific
and social programs. We also appreciate the sustained programmatic
support and guidance of Peter Ward and Stephen Kohl, who administer
Project 02.05-81 "Ichthyology and Aquaculture" for the USFWS, Office of
International Affairs. The committee and delegates are also grateful for
the hospitality of the directors and staff at the National Aquarium in
Baltimore for hosting a group social excursion to this world-class
facility.

**[Conference Program:]{.underline}**

**[Sunday, 21 September:]{.underline}**

4:00 **[Registration]{.underline}** Clarion Inn, Shepherdstown, WV

6:30 **[Welcome Reception and Dinner]{.underline}**

> National Fish Health Research Laboratory

Kearneysville, West Virginia

Panek Scientific Director, National Fish Health Research Laboratory

Leetown, WV

Faisal Director, Living Oceans Foundation;

> Michigan State University, East Lansing, Michigan

Shchelkunov Russian, co-chairman, Laboratory of Icthyopathology

All-Russian Research Institute of Freshwater Fisheries

Cipriano American Co-Chairman

> National Fish Health Research Laboratory -- Leetown, West Virginia

**[Dinner]{.underline}** Boneless chicken breast with white wine garlic
sauce, grapes and

> mushrooms, beef tips burgundy over noodles, mashed potatoes,
>
> honey glazed carrots, French cut waxed green beans, variety of
>
> salads, deserts, coffee, iced tea, soft drinks

**[Monday, 22 September:]{.underline}**

7:00 **[Breakfast]{.underline}** Fresh fruit cocktail, scrambled eggs,
bacon, pancakes breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
>
> tea, milk

8:30 Bullock A Brief History Of The Leetown West Virginia Laboratory 1

9:00 Karaseva Pathologies Of Demersal Fishes In The Barents Sea And
Adjacent 2

Waters

9:30 Lartseva The Volga Delta And Northern Caspian Sea: Fishing, Fish
Health 3

> And Disease

10:00 **[Break]{.underline}** Coffee and Tea

10:30 Karmanova Health Of Pacific Salmonids Held Under Natural And
Artificial 4

> Conditions Of Reproduction In Kamchatka

11:00 Kuznetsova Diseases of Whitefish (Coregonidae) In The Russian
North-West 5

> And North Caucasus

11:30 Shchelkunov Aquaculture And Fish Diseases In Russia And Former
Soviet Union: 6

> Recent History And Current Situation

12:00 **[Lunch]{.underline}** Vegetable Lasagna, house salad, starch and
vegetable, rolls, butter, coffee,

> iced tea

1:30 Cipriano Atlantic Salmon Restoration In New England: Significance
Of 7

> Certain Diseases

2:00 Ottinger Mycobacteriosis And Chesapeake Bay Striped Bass: An 8

Integrated Cooperative Research Program

2:30 Hawke Diagnosis And Management Of Bacterial Diseases In 9

Warmwater Aquaculture

3:00 **[Break]{.underline}** Coffee, Tea, and Iced tea

3:30 Faisal Health And Disease Issues Associated With Populations Of
Fish 10

> In The Great Lakes

4:00 Meyers Disease Transmission From Cultured Salmonids To Wild Fish
Stocks: 11

Perspectives Of The Alaskan Hatchery Program

4:30 Miller, O. United States Department of Agriculture Services Related
To Infectious 12

Salmon Anemia And Spring Viremia Of Carp Aquatic Animal

> Health Programs

5:00 **[Adjourn]{.underline}**

7:00 **[Dinner]{.underline}** Clarion Inn

> Breast of Chicken Kiev, spinach salad with Catalina or peppercorn
> ranch dressings, vegetable and starch, fruit pie coffee and tea

**[Tuesday, 23 September:]{.underline}**

7:00 **[Breakfast]{.underline}** Fresh fruit cocktail, scrambled eggs,
sausage, French toast, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea, milk

8:30 Shchelkunov Theory And Practice Of Establishing Long-Term Cell
Lines From Fish 13

> And Shellfish

####### 9:00 Bowser Retroviral Pathogens And Tumors In Coolwater Fish -- Walleye Dermal 14 

####### Sarcoma

9:30 Buchatsky Immune Sensor Based On Surface Plasmon Resonance For
Express 15

> Control Of Fish Retroviral Infections

10:00 **[Break]{.underline}** Coffee and Tea

10:30 Borissova Isolation Of Infectious Pancreatic Necrosis Virus From
Young Atlantic 16

> Salmon (*Salmo salar* L.) In Russia

11:00 Rudakova Infectious Hematopoietic Necrosis Virus (IHNV):
Repetitive Isolation 17

> From Adult Sockeye Salmon *(Oncorhynchus nerka)* From the River
>
> Bolshaya-Bystraya (Kamchatka)

11:30 Kurath Genetic Typing Of Infectious Hematopoetic Necrosis Virus 18

12:00 **[Lunch]{.underline}** Char-grilled beef kabob, house salad, rice
pilaf, and vegetable, rolls,

> butter, coffee, iced tea

1:30 Oreshkova Diagnosis And Specific Prevention Of Spring Viremia Of
Carp 19

2:00 Pichugina First Report Of Spring Viremia Of Carp Virus At A Fish
Farm In 20

> Moscow Province, Russia

2:30 Golovin Intracytoplasmic Inclusions In Erythrocytes Of Pacific
Salmonids 21

3:00 **[Break]{.underline}** Coffee, Tea, and Iced tea

3:30 Hoffman Keynote Talk: A historical perspectus of fish parasitology

4:30 Faisal The Status And Future Of Aquaculture In Senegal

5:00 **[Adjourn]{.underline}**

7:00 **[Dinner]{.underline}** Bavarian Inn

> Sauerbraten, Choice Beef Roast marinated for one week in Vinegar
>
> and Spices with Red Wine Ginger Sauce, Potato Dumpling and Red
>
> Cabbage, Apple strudel, coffee and tea, iced tea

**[Wednesday, 24 September:]{.underline}**

7:00 **[Breakfast]{.underline}** Fresh fruit cocktail, western scrambled
eggs, ham, Pancakes, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea,
>
> milk

8:30 Bell The United States Fish And Wildlife Service's National Wild
Fish 22

> Health Survey: A New Tool For Fisheries Management

9:00 Blazer A*phanomyces* *invadans* (=*A. piscicida*): A Widespread
Problem In Wild 23

> And Cultured Fishes

9:30 Abt Marine Mammal Stranding Response: Conservation Through
Cooperation 24

10:00 **[Break]{.underline}** Coffee and Tea

10:30 Dunn A Brief Review Of Some Important And Emerging Bacterial
Diseases 25

Of Pinnipeds And Cetaceans

11:00 Whitaker Emerging Challenges In Public Aquaria 26

11:30 **[Lunch]{.underline}** Roasted turkey served with a three
mushroom sauce, house salad,

fresh starch, and vegetable, rolls, butter, coffee, iced tea

1:00 **[Bus Trip]{.underline}** National Aquarium and Inner Harbor,
Baltimore, Maryland

(Dinner on your own)

8:00 Return to the Clarion Inn, Shepherdstown, West Virginia

**[Thursday, 25 September:]{.underline}**

7:00 **[Breakfast]{.underline}** Fresh fruit cocktail, scrambled eggs,
sausage, pancakes, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea,
>
> milk

8:30 Vaschenko DDT In Bottom Sediments And Liver Of Plaice
*Pleuronectes* 27

> p*innifasciatus:* The Factor Affecting Fish Health In Amur Bay (Peter
>
> The Great Bay, Sea Of Japan)

9:00 Fisher Global Climmate And Large-Scale Environmental Influences On
Aquatic 28

> Animal Health

9:30 Lukin Tumors Of Corregonid Fishes In The River Pechora 29

10:00 **[Break]{.underline}** Coffee and Tea

10:30 Vaschenko Disturbance Of Reproduction In Bottom Invertebrates And
Fish 30

> Inhabiting Polluted Coastal Waters Near Vladivostok (Amursky Bay,
>
> Sea Of Japan)

11:00 Syasina Tumors In Fish From Peter The Great Bay, Sea Of Japan 31

11:30 Blazer Endocrine Disruption in Aquatic Environments: Effects on
Fish 32

> Populations

12:00 **[Lunch]{.underline}** Trio salad plate: Shrimp, chicken and tuna
salads on a bed of greens,

> garnished with sweet breads and cottage cheese, rolls, butter, coffee,
>
> iced tea

1:30 Karmanova Myxosporidia And Microsporidia From Salmon On Kamchatka
33

2:00 Densmore Whirling Disease in the United States: A Shifting
Perspective in 34

the 21^st^ Century Whirling Disease

2:30 Badmaeva Pathological Morphology In Kidney Tissue Of Siberian Roach
(*Rutilus* 35

> *rutilus* Lacustris) Infected With Myxosporidians From Lake Baikal

3:00 **[Break]{.underline}** Coffee, Tea, and Iced tea

3:30 Lartseva Parasites Of Fish In The Volga-Caspian Region: Organisms
Pathogenic 36

> For Humans And Animals

4:00 Boutorina Similarities And Differences In Fauna Of Parasites Of
Chars 37

> In The Paleacrctic (Eurasia) And Newarctic (North America)

4:30 Karaseva Health Protection Of Fish In The European North Of Waters
38

5:00 **Adjourn**

5:30 **[Dinner]{.underline}** LSC/NFHRL Fishing Derby Pavilion

#####  

##### Barbecued Ribs and Chicken, Macaroni and cheese, scalloped 

##### potatoes, corn, selection of salads, desserts, coffee, iced tea, 

##### and soft drinks 

**[Friday, 26 September]{.underline}**

7:00 **[Breakfast]{.underline}** Fresh fruit cocktail, scrambled eggs,
bacon, French toast, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea,
>
> milk

8:30 Garrison Disease On Coral Reefs -- What We Know? 39

9:00 Smith Recent Observations On Aspergillosis Of Gorgonians 40

####### 

#######  9:30 Lartseva Integrated Research Into Species Of The *Ctenophora* Family: *Beroe* 41

####### *ovata* And *Mnemiopsis leidyi*

10:00 **[Break]{.underline}** Coffee and tea

10:30 Kern Introduced Invasive Species: Shrimp, Shrimp Diseases, And
Their 42

Impacts On Native And Cultured Shrimp

11:00 Burnett Impacts Of Hypoxia On The Resistance Of Penaeid Shrimp To
Bacterial 43

> Pathogens

11:30 Dixon Possible Roles For The Enzymes Produced By The Intestinal
Obligate 44

> Anaerobic Microflora Isolated From Shrimp Litopenaeus vannamei

12:00 **[Lunch]{.underline}** Breast of chicken served with three
mushroom sauce, house salad,

> starch and vegetable, rolls, butter, coffee, iced tea

1:30 Robohm Mocrobial Disease And Population Status Of The American
Lobster 45

> (*Homarus americanus*)

2:00 Starliper Pathogen And Disease Issues Associated With Imperiled
Populations 46

Of Freshwater Mussels

2:30 Guseva Monitoring *Flexibacter-Flavobacterium* Infections In
Sturgeon 47

3:00 **[Break]{.underline}** Coffee, Tea, and Iced tea

3:30 Asadchaya Bacterial Diseases Of Herbivorous Fishes In Belarus 48

4:00 Sergeenko Characterization Of Strains Of *Aeromonas salmonicida*
subsp. 49

> s*almonicida* Isolated From Salmon Spawners In Fish Hatcheries
>
> On Kamchatka

4:30 Brown Molecular Mechanisms Of Host-Pathogen Interactions Between 50

> *Aeromonas salmonicida* And Atlantic Salmon, *Salmo salar*

5:00 **Adjourn**

7:00 **[Banquet]{.underline} Clarion Inn**

California Green salad with peppercorn ranch or raspberry vinaigrette

> Dressings, Beef bourguignonne, Seafood Newburg with rice, & carved
>
> roast top round of beef. Vegetable and starch, Assortment of Pies,
> cakes, mousse and fresh fruit, rolls, butter, wine, coffee, tea, iced
> tea
>
> [Special guests:]{.underline}
>
> Dr, Charles Groat, Director, United States Geological Survey
>
> Mr. Steven Kohl, USFWS Office of International Affairs
>
> [Dance]{.underline}

11:30 **[Adjourn]{.underline}**

**[Saturday, 27 September:]{.underline}**

7:00 **Breakfast** Fresh fruit cocktail, western scrambled eggs, ham,
pancakes, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea,
>
> milk

9:00 Kondratieva Immune Parameters Of Common Species Of Aquatic Animals
51

9:30 Miller, N. Advances In Fish Immunology:Identification And
Characterization 52

> Of Natural Killer-Like Cells In Channel Catfish (*Ictalurus
> punctatus*)

10:00 **[Break]{.underline}** Coffee and Tea

10:30 Kaattari Vaccine Design And The Analysis Of Long-Lived Plasma Cell
53

> ProductionAnd Regulation

11:00 Golovin Stress In Fishes: Diagnosis And Corrective Treatment With
*Piscin* 54

11:30 Suprun Aspects Of Application Of Vitamin-Coenzyme Fungal Complex
At 55

Early Stages Of Fish Ontogenesis

12:00 **[Lunch]{.underline}** Tenderloin of beef plate garnished with
horseradish cream and herb

> green sauce, served with grilled roasted vegetables, rolls, butter,
> coffee,
>
> iced tea

1:30 Chernysh Metabolic Processes In The Livers Of Carp Cultured In Warm
Water 56

2:00 Parkhomenko Influence Of Fodder Additions That Lower Lipogenesis On
The 57

> Development And Survival Of Fish Maintaines In Warm Water

2:30 Honeyfield Thiamine Deficiency Complex In Fish And Aquatic Animals
58

3:00 **[Adjourn]{.underline}**

6:30 **[Dinner]{.underline} Anvil Restaurant**

Choice of Prime Rib au jus, Maryland Crab Cakes, or Chicken picatta

House salad, Chef's choice of starch and vegetable, coffee, tea, soft
drinks,

Choice of Key Lime pie, Kentucky Derby pie, Snicker's pie or Cheesecake

8:30 **[Ghost Tour]{.underline}**

Harpers Ferry, West Virginia

**[Sunday, 28 September:]{.underline}**

8:00 **[Breakfast]{.underline}** Fresh fruit cocktail, scrambled eggs,
bacon, French toast, breakfast

> potatoes, assorted bakery items, butter, jelly, jams, syrup, coffee,
> tea,
>
> milk

10:30 **Optional Tour of Washington DC**

#### Monday, 29 September

12:00 F**inal Departures for Airports**