Luke Iwanowicz

320 Pulpit Hill Road

Amherst, MA 01002

413.549.9205

Luke_Iwanowicz@usgs.gov

**EDUCATION:**

*Present* **PhD Student**

University of Massachusetts at Amherst

> **Classes:**
>
> Experimental Endocrinology Interpretation of Ecological Data
>
> Physiology of Reproduction Methods in Environmental Toxicology
>
> Comparative Animal Physiology Applied Biostatistics
>
> Immunology Infection and Immunity

*2000* **M.S. Aquaculture/Fisheries**

**Concentration in Fish Health**

> University of Arkansas at Pine Bluff

##### *1997* B.S. Fisheries Biology/ Biology minor

University of Massachusetts at Amherst

##### CONTINUING EDUCATION:

*2003* Understanding and managing viral diseases in fish

AFS Fish Health Section and Western Fish Disease Conference

*2003* Molecular Biology -- The basics

> 28^th^ Eastern Fish Health Workshop

**PROFESSIONAL BIOLOGY EXPERIENCE:**

*12/ 2002 -* **United States Geological Survey, Student trainee -
Biology**

*Present* **National Fish Health Research Laboratory**

> *Leetown, WV*

*09/ 2002 -* **University of Massachusetts at Amherst, Graduate research
assistant**

*Present* **Silvio O. Conte Anadromous Fish Research Laboratory**

*Turners Falls, MA*

*05/ 2001 -* **Virginia Institute of Marine Science, Scientist II
(Marine Scientist Senior)**

*09/ 2002* **National Fish Health Research Laboratory**

######## Leetown, WV

*06/ 2000 -* **Johnson Controls World Services, Biologist II**

*04/ 2001* **National Fish Health Research Laboratory**

> *Leetown, WV*

*06/ 2000* **Cooperative Extension Fish Diagnostic Laboratory, Temporary
diagnostician**

> *Lonoke, AR*

*01/ 1998 -* **University of Arkansas at Pine Bluff, Graduate teaching/
research assistant**

*05/ 2000* **Fish Diagnostic Laboratory**

> *Pine Bluff, AR*

**OTHER EXPERIENCE:**

*05/ 2002 -* **Private Consulting (Expert Witness)**

*01/ 2003* **Wolfson, Keenan, Cotton and Meaggher Attorneys at Law**

> *Worcester, MA*

*09/ 1997 -* **Triton Regional High School, Full-time substitute**

### 12/ 1997 Byfield, MA

*01/ 1997 -* **AquaFuture, Student internship**

*05/ 1997 Turners Falls, MA*

*01/ 1995 -* **Richard Cronin National Salmon Station, Volunteer**

*05/ 1997* *Sunderland, MA*

*04/ 1997* **Division of Inland Fisheries, Volunteer**

#### Southwick, MA

*02/ 1992 -* **Self Employed Commercial Shell Fisherman**

######## 12/ 1997 Newbury, MA

**PRESENTATIONS:**

Calcein AM Release-based Cytotoxic Cell Assay for Fish Leucocytes

July 2003

> AFS Fish Health Section and Western Fish Disease Conference
>
> *Seattle, WA*

A Fluorescence Based Cytotoxic Assay for Fish Leucocytes Using Calcein
AM Labeled Adherent Target Cells

March 2002

> The 27^th^ Annual Eastern Fish Health Workshop
>
> *Charleston, SC*

A Fluorescence-Based Cytotoxic Assay for Fish Leukocytes Using EPC
Target Cells

April 2001

> The 26^th^ Annual Eastern Fish Health Workshop
>
> *Shepherdstown, WV*

An Overview of Viruses Isolated from Fathead Minnows, Golden Shiners,
and Grass Carp Raised in Arkansas

June 2000

> Informational County Extension Seminar
>
> *Lonoke, AR*

A Unique, Bacilliform, RNA Virus Isolated from Fathead Minnows
(*Pimephales promelas)*

April 2000

> The 25^th^ Annual Eastern Fish Health Workshop
>
> *Plymouth, MA*

A Summary of Viruses Isolated from Cultured Cyprinids at the UAPB Fish
Diagnostic Laboratory

March 2000

> 7^th^ Biennial Southern Fish Disease Conference
>
> *Louisiana State University*

Western Blot and Reciprocal RNA-RNA Hybridization Analysis of
Aquareoviruses Isolated from Cyprinids Cultured in Arkansas, USA

March 2000

> UAPB Research Forum
>
> *University of Arkansas at Pine Bluff*

Golden Shiner Virus-like Isolates from Atypical Epizootics Occurring in
Cool Water

March 1999

> UAPB Research Forum
>
> *University of Arkansas at Pine Bluff*

Golden Shiner Virus-like Isolates from Atypical Epizootics Occurring in
Cool Water

March 1999

> The 24^th^ Annual Eastern Fish Health Workshop
>
> *Atlantic Beach, NC*
>
> **Causes of Chronic Mortality in Bait and Ornamental Fish**
>
> February 1999
>
> Annual Meeting of the Baitfish Growers of Arkansas

# *Lonoke, AR County Extension Building*

# **Identification of a Putatively Novel Virus in Fathead Minnows *(Pimephales promelas)***

# April 1998

> Student Chapter of the American Fisheries Society Meeting
>
> *University of Mississippi*
>
> March 1998
>
> 6^th^ Biennial Southern Fish Disease Conference
>
> *Auburn University*

**PUBLICATIONS:**

> L Iwanowicz, C Densmore and C Ottinger (2003) Calcein AM
> released-based cytotoxic cell assay for fish leucocytes. *Fish &
> Shellfish Immunology*. *In press.*
>
> L R Iwanowicz and A E Goodwin (2002) Characterization of a new
> bacilliform fathead minnow rhabdovirus that produces syncytia in
> tissue culture. *Archives of Virology* 147: 889 -- 916.
>
> Christine L. Densmore, Christopher A. Ottinger, Vicki Blazer and Luke
> R. Iwanowicz (2002). Immunomodulation and disease resistance in
> *Myxobolus Cerebralis* infected rainbow trout. Proceedings of the
> 8^th^ Annual Whirling Disease Symposium.
>
> L R Iwanowicz, A E Goodwin and J Harshbarger (2001) Embryonal
> rhabdomyosarcoma of the giant gourami, *Colisa fasciata* (Bloch &
> Schneider). *Journal of Fish Diseases* 24:177-179*.*
>
> L R Iwanowicz, A E Goodwin and N Heil (2000) A small RNA virus
> isolated from apparently healthy wild sandbar shiners, *Notropis
> scepticus*. *Journal of Fish Diseases* 23: 349-352.
>
> Christine Densmore, Christopher Ottinger, Vicki Blazer Luke Iwanowicz
> and Dave Smith. Immunomodulation and disease resistance in
> post-yearling Rainbow trou (*Oncorynchus* mykiss) in fected with
> *Myxobolus cerebralis*, the causative agent of whirling disease.
> *Journal of Aquatic Animal Health*. *In review*.
>
> M. McIntire, L R Iwanowicz, and A E Goodwin. Molecular analysis and
> comparison of aquareoviruses from grass carp (*Ctenopharyngodon
> idella*) and golden shiners (*Notropis crysoleucas*). *In
> preparation.*

######### AFFILIATIONS:

######### American Fisheries Society, 1994-1996; 2001 - Present

> Fish Health Section, 2001 -- Present
>
> American Society of Microbiology, 2002 - Present
>
> Society of Integrative and Comparative Biology, 2003 - Present