Voluntary Report - Public distribution

**Date:** 11/12/2008

**GAIN Report Number:** CH8104

CH0000

**China, Peoples Republic of**

**FAIRS Subject Report**

**Oilseed Standards**

**2008**

**Approved by:**

![](media/image1.wmf)Mark Petry

AgBeijing

**Prepared by:**

Wu Bugang

**Report Highlights:**

On July 3, 2008, China notified the WTO of the National Standard
GB-1532-2006 \"National Standard for Soybeans\" as TBT/N/CHN/402. This
standard specifies the relevant terms and definitions, classifications,
quality requirements, test methods, and requirements for labeling,
packaging, transportation and storage of soybeans. GB/T 5512 Inspection
of Grain and Oilseeds - Methods for Determination of Crude Fat is
referenced in that standard and published here as a reference in
reviewing TBT/N/CHN/402. This report is an UNOFFICIAL translation of
GB/T 5512.

Includes PSD Changes: No

Includes Trade Matrix: No

Annual Report

Beijing \[CH1\]

\[CH\]

**Executive Summary**: On July 3, 2008, China notified the WTO of the
National Standard GB-1532-2006 \"National Standard for Soybeans\"
(Replacing GB 1352-1986) as TBT/N/CHN/402. This standard specifies the
relevant terms and definitions, classifications, quality requirements,
test methods, and requirements for labeling, packaging, transportation
and storage of soybeans. This standard also applies to testing,
evaluation and identification of the quality of commercial soybeans. The
date for submission of final comments to the WTO is September 3, 2008.
The proposed date of adoption is 90 days after circulation by the WTO
Secretariat (October 3, 2008) and the proposed date of entry into force
is 6 months after adoption (January 3, 2009). This is notified as GAIN
Report CH8066.

One of the measures that is referenced in the proposed National Standard
is GB/T 5512 Inspection of Grain and Oilseeds - Methods for
Determination of Crude Fat. This standard has not been notified to the
WTO. This National Standard, along with other standards published in
GAIN Reports CH8097-CH8105, is being published so that GB-1532-2006
\"National Standard for Soybeans\" TBT/N/CHN/402 can be reviewed with
this additional pertinent information.

Thanks go to the United States Soybean Export Council -- International
Marketing and the U.S. Grains Council for their support in translating
this measure.

**BEGIN TRANSLATION**

**National Standard of the People's Republic of China**

**GB 5512-85**

**Inspection of Grain and Oilseeds - Methods for Determination of Crude
Fat**

**Issued on Nov. 2, 1985 Implemented on July 1, 1986**

**Approved by the Standardization Administration of P.R.C.**

This standard is applicable to determination of the crude fat content of
commodity grain and oilseeds.

**1** **Soxhlet Extractor Method**

1.1 Instruments and Apparatus

1.1.1 Analytical balance: sensitivity 0.0001g;

1.1.2 Electric thermostat;

1.1.3 Electric thermostatic water bath;

1.1.4 Disintegrator, mortar;

1.1.5 Desiccator with allochroic silica gel

1.1.6 Filter paper cylinder

1.1.7 A set of Soxhlet Extractor (all parts must be cleaned and dried at
the temperature of 105℃, and the extraction flask must be dried to
constant weight);

1.1.8 Jar, absorbent string, absorbent cotton, absorbent bank sand

1.2 Reagent - Absolute ether (A.R)

1.3 Sample Preparation

1.3.1 Take 30\~50g cleaned and impurity-free test sample from cereal and
bean (except peanut) respectively, grind them all and then put them into
the jar for standby by screening with a round hole sieve with a diameter
of 1.0mm.

1.3.2 Take 20g cleaned and impurity-free test sample from the
small-sized oilseeds such as sesame, rapeseeds and linseeds respectively
and then put them into the jar for standby.

1.3.3 Take 30\~50g cleaned and impurity-free sample from the large-sized
oilseeds such as peanut in shell, castor bean, sunflower seeds and
tea-seeds, after the impurity is removed, decorticate them one by one
and weigh the kernel and hull respectively and then calculate the
percentage of the total amount of kernel. Finally, cut the kernel into
pieces and put them into the jar for standby.

1.4 Operation Methods

1.4.1 Test sample binding up: weigh up 2\~5g test sample from the
standby sample prepared with a bake box and dry them at a temperature of
105℃ for 30 min. Put them into the mortar while they are hot and add in
about 2g absorbent bank sand and grind them together. After they have
turned into oily matter, put them into the filter paper cylinder cleanly
(put a layer of absorbent cotton into the bottom of the cylinder and dry
the cylinder at the temperature of 105℃ for 30 minutes). Dip a little
ether with absorbent cotton to wipe away the test sample and fat
remaining in the mortar and put them into the filter paper cylinder.
Finally, stuff the upper part of the filter paper cylinder with
absorbent cotton to hold down the test sample.

1.4.2 Extraction and drying: install the extractor properly and put the
filter paper cylinder with test sample into the extractor cylinder. At
the same time inject the ether above the height of the siphon pipe and
inject the ether again to 2/3 height of the siphon pipe after the ether
drained. Cram a small piece of absorbent cotton into the back cut of the
condensate tube gently; open the water inlet pipe of the condensate tube
for heating and extraction. The heating temperature shall be based on
the reflux of the ether to be 120\~150 drops/min and 7 times reflux/h.
The time for extraction shall depend on the oil content in test sample,
generally more than 8 hours and extract till there is no oil trace in
ether in extraction tube at all when checked by the sheet glass (spot
test).

> After all fats are extracted, take the filter paper cylinder out by
> long-arm forceps and reheat the ether to make it reflow twice. Then
> take the ether back. Take down the condensate tube and the extract
> cylinder and then deplete the ether remained in the extract flask by
> heating. Clean the outside of the extract flask by absorbent cotton
> dipping with ether, then dry the extract flask at the temperature of
> 105℃ for 90 min firstly, then for 20min till it was dried to constant
> weight (if the weight difference between the former and the latter is
> within 0.0002g, it will be regarded as constant weight). The increased
> weight of the extract flask is the weight of the crude fat.

1.5 Calculation of Results

> The content of crude fat under wet basis, dry basis and at the
> standard aquatic impurity rate shall be calculated as formula (1), (2)
> and (3):
>
> Crude fat (％in wet basis) =
> $\frac{W_{1}}{W} \times \text{100}$\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\--(1)
>
> Crude fat (％in dry basis) =
> $\frac{W_{1}}{W\left( \text{100} - M \right)} \times \text{10000}$\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\--
> (2)
>
> Crude fat (％at the standard aquatic impurity rate) =
> $\frac{W_{1}\left( \text{100} - M_{\text{std}} \right)}{W\left( \text{100} - M \right)} \times \text{100}$\-\-\--(3)
>
> Where: *W*~1~---the weight of crude fat, g;
>
> *W*---the weight of test sample, g;
>
> *M*---the percentage of moisture content in test sample, %;
>
> *M*~std~---the sum of standard test samples moisture content and the
> standard impurity, %.
>
> The allowable deviation between dual test results shall be no more
> than 0.4% for grain and oilseeds and no more than 0.2% for soybean.
> Figure out the average number of the dual test results which will be
> the measured result. Take the first digit after the decimal point for
> the measured result.
>
> If the crude fat of the unhulled oilseeds is determined, then it must
> be converted by formula (4) and (5):
>
> Crude fat of the unhulled oilseeds (％in wet basis) =
> $\frac{N \times A}{\text{100}}$\-\-\-\-\-\-\-\-\-\-\-- (4)
>
> Crude fat of the unhulled oilseeds (％in dry basis) =
> $\frac{N \times A}{\text{100} - M}$\-\-\-\-\-\-\-\-\-\-- (5)
>
> Where:
>
> *N*---the percentage of the crude fat of the unhulled oilseeds' kernel
> in wet basis, %;
>
> *A*---the percentage of the total kernel amount of the unhulled
> oilseeds, %;
>
> *M*---the percentage of moisture content in unhulled oilseeds, %.
>
> Note: if no filter paper cylinder is available, take the filter paper
> with a length of 28cm and a width of 17cm and roll the paper into a
> shape of cylinder with a test tube with a diameter of 2cm along the
> long edge of the paper. Draw the cuvette out of the paper cylinder to
> half height, flatten the evacuated part of the paper cylinder, and
> fold it to make it tightly closed to the external layer of the
> cuvette. Tie the paper cylinder with absorbent string, fold the lower
> angle upwards and press it into a round bottom. Then a filtration
> paper cylinder is formed with a diameter of 2.0cm and a height of
> 7.5cm after taking the cuvette out.

**2. Drip Extraction Method**

2.1 Instruments and Apparatus

2.1.1 Drip extractor;

2.1.2 Other instruments and apparatus are the same with those in 1.1.

2.2 Reagent

> All reagents are same with those in 1.2.

2.3 Sample Preparation

> Method of sample preparation is same with 1.3.

2.4 Operation Method

> The instruments treatment is the same with that described in 1.4. Put
> the sample package into the extract pipe and extract the fat by ether.
> Take the sample package out after all fat has been extracted and then
> close the glass piston for reflowing. The ether can be taken back by
> continuous heating. The other methods are the same as those in 1.4.
>
> Note: the refabrication of the recycled ether can be divided into 3
> steps:
>
> ① Remove the peroxide: inject the ether into the separating funnel and
> add in 10% ferrous sulfate solution accounting for 1/5 of the amount
> of ether (take 100g ferrous sulfate and dissolve it in 600ml water and
> add in 30ml concentrated sulfuric acid for acidification, then dilute
> the solution to 1000ml by water) to sufficiently mix. Discharge the
> solution after standing for clarification
>
> ② Remove the ethanol: add in 10% potassium hydroxide solution
> accounting for 1/5 of the amount of the ether, and stand after
> oscillation and washing. Then discharge the solution, only 2\~3 times
> rewashing is necessary.
>
> ③ Remove the moisture and distillation: put appropriate amount
> (1/10\~1/5 of the amount of ether) of granule anhydrous calcium
> chloride into the ether flask and place it for 24 hours, rock the
> flask from time to time. Take clear solution from the upper layer for
> distillation and collect the cut fraction in the temperature range of
> 33 ℃ to 37℃. The ether adopter shall be cooled by ice or cold water,
> and connect the adopter with a safety flask simultaneously and exhaust
> the gas in the flask out of room or into the sewer.

**Additional Explanation**

This national standard was proposed by the Ministry of Commerce of the
P.R.C.

This standard was drafted by the Grain Storage and Transport Bureau, the
Ministry of Commerce, P.R.C.

Major draftsmen of this standard are Gao Xiuwu, Yang Haoran, Wu Yanxia
and Lu Guifen.