**Appendix C.** Analytical Methods for the University of Maine
Laboratory.

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*Analyte Method Reference*

pH, closed cell Electrode Hillman *et al.*^6^, EPA 19.0^5^

pH, aerated Electrode Hillman *et al.*^6^, EPA 5.0^5^

Specific conductance Wheatstone bridge EPA 120.1^2^,EPA 23.0^5^

True color Spectrophotometer, 457.5 nm EPA 110.2^2^

ANC Gran Titration Hillman *et al.*^6^, EPA 5.0^5^

Anions: Cl, NO~3~, SO~4~ Ion chromatography EPA 300.0^1^

Pre 1999 analysis methods for Ca, Mg, Na, K, and Al

Calcium AAS with N~2~0-acetylene flame EPA 215.1^2^

Magnesium AAS with N~2~0-acetylene flame EPA 242.1^2^

Sodium AAS with air-acetylene flame EPA 258.1^2^

Potassium AAS with air-acetylene flame EPA 273.1^2^

Aluminum (total) AAS with graphite furnace EPA 200.9^1^

1999 to 2003 methods for Ca, Mg, Na, K, and Al

Calcium Inductively Coupled Atomic Emission Spectroscopy (ICP) EPA
200.15^10^

Magnesium Inductively Coupled Atomic Emission Spectroscopy (ICP) EPA
200.15^10^

Sodium Inductively Coupled Atomic Emission Spectroscopy (ICP) EPA
200.15^10^

Potassium Inductively Coupled Atomic Emission Spectroscopy (ICP) EPA
200.15^10^

Aluminum (total) Inductively Coupled Atomic Emission Spectroscopy (ICP)
EPA 200.15^10^

2003 to present methods for Ca, Mg, Na, K, NH~4~

Calcium Ion Chromatography (IC) ASTM D 6919-03^11^

Magnesium Ion Chromatography (IC) ASTM D 6919-03^11^

Sodium Ion Chromatography (IC) ASTM D 6919-03^11^

Potassium Ion Chromatography (IC) ASTM D 6919-03^11^

Ammonium Ion Chromatography (IC) ASTM D 6919-03^11^

2003 to present methods for Al

Aluminum (total) AAS with graphite furnace EPA 200.9^1^

Dissolved Organic Carbon IR C analyzer, persulfate oxidation EPA
415.1^2^

Ammonium (prior to 2003) Autoanalyzer EPA 9.0^5^ and

Bran & Luebbe 780-86T^7^

Silica Autoanalyzer EPA 22.0^5^ and

Bran & Luebbe 785-86T^7^

Total Hg CVAFS EPA 1631^8^

dual gold trap

MethylHg Distillation, Aqueous

> Ethylation, Purge and Trap,
>
> and CVAFS EPA 1630^9^

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AAS=atomic absorption spectrophotometry IR=Infrared Spectrophotometry

CVAFS=cold vapor atomic fluorescence spectrometry

Method references:

^1.^ Methods for the Determination of Inorganic Substances in
Environmental Samples, EPA 600/R-93-100, 1993.

^2.^ Methods for Chemical Analysis of Water and Wastes, EPA
600/4-79-020, 1979, Revised 1983.

^3.^ Standard Methods for Examination of Water and Wastewater, 18th ed.
1992.

^4.^ Methods for the Determination of Metals in Environmental Samples,
EPA 600/4-91/010, 1991, Supplement 1, EPA 600/R-94/111, 1994.

^5.^ Handbook of Methods for Acid Deposition Studies: Laboratory
Analysis For Surface Water Chemistry, EPA 600/4-87-026, 1987.

^6.^ Hillman,D.C., J. Potter, and S. Simon, 1986. Analytical methods for
the National Surface Water Survey, Eastern Lake Survey.
EPA/600/4-86/009, EPA Las Vegas.

^7.^ Bran & Luebbe Manual

^8.^ Mercury in Water by Oxidation, Purge and Trap, and Cold Vapor
Atomic Fluorescence Spectrometry, EPA 821-R-99-005, 1999

^9.^ Methyl Mercury in Water by Distillation, Aqueous Ethylation, Purge
and Trap, and Cold Vapor Atomic Fluorescence Spectrometry, EPA Draft,
1998

^10^ EPA 200.15, Determination of Metals and Trace Elements in Water by
Ultrasonic Nebulization Inductively Coupled Plasma-Atomic Emission
Spectrometry. Rev. 1.2, 1994.

^11^ ASTM D 6919-03 Standard Test Method for Determination of Dissolved
Alkali and Alkaline Earth Cations and Ammonium in Water and Wastewater
by Ion Chromatography. Annual Book of ASTM Standards, Vol 11.01, 2003.