REPORTS OF THE COORDINATORS

Overall coordinator's report

**Udda Lundqvist**

**SvalöfWeibull AB**

**SE-268 81 Svalöv, Sweden**

**e-mail: <udda@ngb.se> or udda@nordgen.org**

Since the latest overall coordinator's report in Barley Genetics
Newsletter Volume 35, no changes of the coordinators have been reported.
I do hope that most of you are willing to continue with this work and
provide us with new important information and literature search in the
future. Please observe some address changes have taken place since the
last volume of BGN.

As it became decided at the 9th International Genetic Barley Symposium
in Brno, 2004, the current system and trait coordination should continue
but with a view towards whole genome coordination. Bill Thomas and Dave
Marshall from the Scottish Crop Research Institute, Invergowrie, Dundee,
UK, are investigating the potential of modernizing the overall system
and integrating all types of current and historic data collections into
a single, combined database. They are working on this subject.

In this connection I also want to call upon the barley community to pay
attention on the AceDB database for 'Barley Genes and Barley Genetic
Stocks'. It contains much information connected with images and is
useful for barley research groups inducing barley mutants and looking
for new characters. It gets updated continuously and some more images
are added to the original version. Also the germplasm part is under
revision. The searchable address is:
[www.untamo.net/bgs](http://www.untamo.net/bgs)

**List of Barley Coordinators**

**Chromosome 1H (5):** Gunter Backes, Department of Agricultural
Sciences, The Royal Vetenary and Agricultural University,
Thorvaldsensvej 40, DK-1871 Fredriksberg C, Denmark. e-mail:
\<guba[\@kvl.dk\>]{.underline}

**Chromosome 2H (2):** Jerry. D. Franckowiak, Department of Plant
Sciences, North Dakota State University, P.O.Box 5051, Fargo, ND
58105-5051, USA. FAX: +1 701 231 8474; e-mail:
\<[j_franckowiak@ndsu.nodak.edu]{.underline}\>

**Chromosome 3H (3):** Luke Ramsey, Cell and Molecular Genetics
Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2
5DA, United Kingdom. FAX: +44 1382 562426. E-mail:
\<Luke.Ramsey@scri.sari.ac.uk\>

**Chromosome 4H (4):** Brian P. Forster, Cell and Molecular Genetics
Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2
5DA, United Kingdom. FAX: +44 1382 562426. e-mail:
\<[bforst@scri.sari.ac.uk]{.underline}\>

**List of Barley Coordinators (continued)**

**Chromosome 5H (7):** George Fedak, Eastern Cereal and Oilseed Research
Centre, Agriculture and Agri-Food Canada, ECORC, Ottawa, ON, Canada K1A
0C6, FAX: +1 613 759 6559; e-mail: \<[fedakga@agr.gc.ca]{.underline}\>

**Chromosome 6H (6):** Duane Falk, Department of Crop Science,
University of Guelph, Guelph, ON, Canada, N1G 2W1. FAX: +1 519 763 8933;
e-mail: \<[dfalk@uoguelph.ca]{.underline}\>

**Chromosome 7H (1):** Lynn Dahleen, USDA-ARS, State University Station,
P.O. Box 5677, Fargo, ND 58105, USA. FAX: + 1 701 239 1369; e-mail:
\<[DAHLEENL@fargo.ars.usda.gov]{.underline}\>

**Integration of molecular and morphological marker maps:** Andy
Kleinhofs, Department of Crop and Soil Sciences, Washington State
University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail:
\<[andyk@wsu.edu]{.underline}\>

**Barley Genetics Stock Center:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<[anhang@uidaho.edu]{.underline}\>

**Trisomic and aneuploid stocks:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<[anhang@uidaho.edu]{.underline}\>

**Translocations and balanced tertiary trisomics:** Andreas Houben,
Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3,
DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail:
\<houben@ipk-gatersleben.de\>

**Desynaptic genes:** Andreas Houben, Institute of Plant Genetics and
Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany.
FAX: +49 39482 5137; e-mail: <houben@ipk-gatersleben.de>

**Autotetraploids:** Wolfgang Friedt, Institute of Crop Science and
Plant Breeding, Justus-Liebig-University, Heinrich-Buff-Ring 26-32,
DE-35392 Giessen, Germany. FAX: +49 641 9937429; e-mail:
\<[wolfgang.friedt@agrar.uni-giessen.de]{.underline}\>

**Disease and pest resistance genes:** Brian Steffenson, Department of
Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper
Buford Circle, St. Paul, MN 55108-6030, USA. FAX: +1 612 625 9728;
e-mail: \<[bsteffen@umn.edu]{.underline}\>

**Eceriferum genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX:.+46 418 667109; e-mail: \<[udda@ngb.se or
udda@nordgen.org]{.underline}\>

**Chloroplast genes:** Mats Hansson, Department of Biochemistry,
University of Lund, Box 124, SE-221 00 Lund, Sweden. FAX: +46 46 222
4534 e-mail: \<mats.hansson@biokem.lu.se\>

**Genetic male sterile genes:** Mario C. Therrien, Agriculture and
Agri-Food Canada, P.O. Box 1000A, R.R. #3, Brandon, MB, Canada R7A 5Y3,
FAX: +1 204 728 3858; e-mail: \<MT[herrien@agr.gc.ca]{.underline}\>

**Ear morphology genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX: +46 418 667109; e-mail: [udda@ngb.se or
udda@nordgen.org](mailto:udda@ngb.se or udda@nordgen.org)

Antonio Michele Stanca: Istituto Sperimentale per la Cerealicoltura,
Sezione di Fiorenzuola d'Arda, Via Protaso 302, IT-29017 Fiorenzuola
d'Arda (PC), Italy. FAX +39 0523 983750, e-mail:
\<[michele@stanca.it]{.underline}\>

**Semi-dwarf genes:** Jerry D. Franckowiak, Department of Plant
Sciences, North Dakota State University, P.O. Box 5051, Fargo, ND
58105-5051, USA. FAX: +1 702 231 8474; e-mail:
\<[j_franckowiak@ndsu.nodak.edu]{.underline}\>

**Early maturity genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX: +46 418 667109; e-mail: \<[udda@ngb.se or
udda@nordgen.org]{.underline}\>

**Biochemical mutants - Including lysine, hordein and nitrate
reductase:** Andy Kleinhofs, Department of Crop and Soil Sciences,
Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509
335 8674; e-mail: \<[andyk@wsu.edu]{.underline}\>

**Barley-wheat genetic stocks:** A.K.M.R. Islam, Department of Plant
Science, Waite Agricultural Research Institute, The University of
Adelaide, Glen Osmond, S.A. 5064, Australia. FAX: +61 8 8303 7109;
e-mail: \<[rislam@waite.adelaide.edu.au]{.underline}\>

**Coordinator's Report: Barley Chromosome 1H (5)**

Gunter Backes

The Royal Veterinary and Agricultural University

Department of Agricultural Sciences

Thorvaldsensvej 40\
DK-1871 Frederiksberg C, Denmark

e-mail: guba@kvl.dk

American six-row malting barleys possess an effective and durable
resistance against spot blotch. In the variety 'Morex' Steffenson *et
al*. (Steffenson *et al*. 1996) had dissected his resistance into a
seedlings resistant on chromosome 7H, one major QTL for adult plant
resistance on chromosome 1H and one minor QTL for adult plant resistance
on chromosome 7H. This was done in a doubled haploid population from the
cross 'Steptoe' × 'Morex', and 'Morex' contributet all alleles for
resistance. In order to confirm these resistance genes, 'Morex'
resistance against spot blotch was investigated in the crosses 'Dicktoe'
× 'Morex' and 'Harrington' × 'Morex' (Bilgic *et al*. 2005).
Additionally, the experiment in the cross 'Steptoe' × 'Morex' was
repeated. While the latter experiment confirmed the QTL found before, no
QTL on chromosome 1H was detected in the other two crosses.

The localistion of QTLs for straw-quality characteristics of barley
under drought stress was the aim of Grando *et al*. (2005). For this
purpose 494 F~7~ recombinant inbred lines were scored in two years and
two locations for acid detergent fiber (ADF), neutral detergent fiber
(NDF), voluntary intake (INT), lignin content (LIC), crude protein (CP )
and digestible organic matter in dry matter (OMD). Additionally, in one
environment, the percentages of blades, sheaths and stems, respectively
(PCB, PCH, PCS) were measured. On chromosome 1H, eight QTLs were found:
one for NDF, INT and CP, one for ADF and PCS, one for PCH, two for INT,
one for LIC and NDF, one for CP and one for INT and ash content.

Peighambari *et al*. (2005) performed a QTL analysis in 72 doubled
haploid lines from the cross Steptoe × Morex for several agronomical
traits scored in two years. On chromosome 1H, four different QTLs were
detected: one for number of seeds per spike, one for the date of spike
inititiation, one for spikes per plant and thousand-seeds-weight and one
for date of flowering and date of maturity.

In order to localize QTLs for different disease resistances, Yun *et
al*. (2005) analysed 104 F~6~-plants from a cross between the
*spontaneum*-line OUH602 and the cultivar 'Harrington'. They phenotyped
the lines for resistance against powdery mildew, leaf scald, Septoria
speckled leaf blotch, net type net blotch and spot blotch. On the short
arm of chromosome 1H, they detected one QTL for powdery mildew (at or
nearby the position of the *Mla*-locus), one QTL for scald and one QTL
for net type net blotch. While the allele conferring resistance for
scald and powdery mildew originated from OUH602, 'Harrington'
contributed the allele for resistance against net type net blotch.

In an advanced backcross population (BC~2~DH) originating from a cross
between the *spontaneum* line ISR42-8 and the variety 'Scarlett', von
Korff *et al*. (2005) detected QTLs for different disease resistances.
On chromosome 1H, they found a major QTL for resistance against powdery
mildew, at or near by the *Mla*-locus. The alleles of the
*spontaneum*-line reduced disease severity by 51.5%.

Hori *et al*. (2005) presented an alternative approach for advanced
backcrosses. They produced both doubled haploid lines and BC~3~F~2~
lines from a same cross between the Japanese malting barley variety
'Haruna Nijo' and the *spontaneum*-line H605. The linkage map was
calculated in the population of doubled haploids and subsequently a QTL
analysis was done in both populations for agronomic and phenotypic
traits. On the short arm of chromosome 1H, one QTL was found for kernel
weight and the number of spikelets per ear in the BC~3~F~2~. On the long
arm of the same chromosome, they detected a QTL for the number of
spikelets per ear in the doubled haploids.

In an attempt to find QTL influencing 'none-parasitic leaf spots'
(NPLS), Behn *et al*. (2005) analysed 536 DH lines from a cross between
the NPLS tolerant barley line 'IPZ 24727' and the variety 'Krona' and
compared them with results published before (Behn *et al*. 2004) from a
cross with the same *ant* line and the variety 'Barke' (all spring
barley varieties). On chromosome 1H, they found a minor QTL
NPLS-tolerance in each of the crosses, but on different regions of the
chromosome. Additionally, they detected three different QTLs for heading
date and two QTLs for plant height on the same chromosome.

Yin *et al* (2005) looked for QTLs representing inputs for a
ecophysiological phenology model predicting flowering time in the cross
'Apex' × 'Prisma': *f*o as the minimum number of days from sowing to
flowering under optimal conditions,. θ~1~ and θ~2~ as the development
stage for the start and the end of the photoperiod-sensitive phase,
respectively, and δ as the parameter characterizing the
photoperiod-sensitivity. On chromosome 1H, they found 3 different loci:
one for the θ~1~, one for *f*o and one for all four parameters.

By addition lines, Nasuda *et al*. (2005) localised totally 701 EST
sequences to the 7 barley chromosomes. Seventy one were assigned to
chromosome 1H.

Rostoks *et al*. (2005) presented an integrated map from three
populations originating from the crosses 'Steptoe'  'Morex', 'Lina' 
HS92 and 'Oregon Wolfe Barley Dominant'  'Oregon Wolfe Barley
Recessive'. Beside 904 RFLP, SSR, and AFLP markers localized before, the
map is enriched by 333 EST unigenes, localized by SNPs, InDels or SSRs
within these genes. For many of these unigenes, up- or down-regulation
under different stress conditions is presented as well as the
localization of the respective homologues in rice. On chromosome 1H, 41
unigenes were localized.

**References**

Behn, A., L. Hartl, G. Schweizer, and G. Wenzel. 2004. QTL mapping for
resistance against non-parasitic leaf spots in a spring barley doubled
haploid population. Theor. Appl. Genet. 108(7): 1229-1235.

Behn, A., L. Hartl, G. Schweizer, and M. Baumer. 2005. Molecular mapping
of QTLs for non-parasitic leaf spot resistance and comparison of
half-sib DH populations in spring barley. Euphytica 141(3): 291-299.

Bilgic, H., B. J. Steffenson, and P. M. Hayes. 2005. Comprehensive
genetic analyses reveal differential expression of spot blotch
resistance in four populations of barley. Theor. Appl. Genet. 111(7):
1238-1250.

Grando, S., M. Baum, S. Ceccarelli, A. Goodchild, F. Jaby El-Haramein,
A. Jahoor, and G. Backes. 2005. QTLS for straw quality characteristics
identified in recombinant inbred lines of a *Hordeum vulgare* x *H.
spontaneum* cross in a Mediterranean environment. Theor. Appl. Genet.
110(4): 688-695.

Hori, K., K. Sato, N. Nankaku, and K. Takeda. 2005. QTL analysis in
recombinant chromosome substitution lines and doubled haploid lines
derived from a cross between *Hordeum vulgare* ssp. *vulgare* and
*Hordeum vulgare* ssp. *spontaneum*. Mol. Breeding 16(4): 295-311.

Korff, M. von, H. Wang, and J. Léon. 2005. AB-QTL analysis in spring
barley. I. Detection of resistance genes against powdery mildew, leaf
rust and scald introgressed from wild barley. Theor. Appl. Genet.
111(3): 583-590.

Nasuda, S., Y. Kikkawa, T. Ashida, K. Sato, A. K. M. R. Islam, K. Sato,
and T. R. Endo. 2005. Chromosomal assignment and deletion mapping of
barley EST markers. Genes & Genetic Systems 80(5): 357-366.

Peighambari, S. A., B. Y. Samadi, C. Nabipour, Gilles, and A. Sarrafi.
2005. QTL analysis for agronomic traits in a barley doubled haploids
population grown in Iran. Plant Sci. 169(6): 1008-1013.

Rostoks, N., S. Mudie, L. Cardle, J. Russell, L. Ramsay, A. Booth, J.
Svensson, S. Wanamaker, H. Walia, E. Rodriguez, P. Hedley, H. Liu, J.
Morris, T. Close, D. Marshall, and R. Waugh. 2005. Genome-wide SNP
discovery and linkage analysis in barley based on genes responsive to
abiotic stress. Mol. Genet. Genom. 274(5): 527.

Steffenson, B. J., P. M. Hayes, and A. Kleinhofs. 1996. Genetics of
seedling and adult plant resistance to net blotch (*Pyrenophora teres*
f. *teres*) and spot blotch (*Cochliobolus sativus*) in barley. Theor.
Appl. Genet. 92(5): 552-558.

Yin, X. Y., P. C. Struik, F. A. van Eeuwijk, P. Stam, and J. J. Tang.
2005. QTL analysis and QTL-based prediction of flowering phenology in
recombinant inbred lines of barley. J. Exp. Bot. 56(413): 967-976.

Yun, S., L. Gyenis, P. Hayes, I. Matus, K. Smith, B. Steffenson, and G.
Muehlbauer. 2005. Quantitative trait loci for multiple disease
resistance in wild barley. Crop Sci. 45(6): 2563-2572.

**Coordinator's report: Chromosome 2H (2)**

J.D. Franckowiak

Department of Plant Sciences

North Dakota State University

Fargo, ND 58105, USA.

e-mail: j.franckowiak@ndsu.nodak.edu

Gottwald *et al*. (2004) reported on an attempt to isolate the gene
controlling a gibberellic-acid insensitive dwarf mutant in barley. The
locus was named *sdw3* and is closely linked to RFLP marker MWG2287 on
2HS near the centromere. The gene symbols *gai* and *GA-ins* were used
for the mutant in line Hv287 in earlier publications (Börner *et al*.,
1999). This region of 2HS is orthologous with a highly conserved region
on rice chromosome 7L. ESTs in this region were used to identify three
putative GA-related ORFs in rice that might correspond to the *sdw3*
locus (Gottwald *et al*., 2004).

Dahleen *et al*. (2005) studied 27 mutants from various sources that
were placed in the brachytic (*brh*) group of semidwarf mutants. Based
on allelism tests and molecular mapping studies using simple sequence
repeat (SSR) markers, the mutants occurred at 18 different loci. Three
of the brachytic mutants were located on chromosome 2H: *ert-t*
(*brh3.y*), *brh4.j*, and *brh10.l*. Several mutants earlier identified
as having a *brh3* phenotype were found to be allelic at the *ert-t*
locus. Since the *ert-t* locus symbol was the symbol first published for
this locus, it will be the recommended symbol. The *ert-t* locus was
positioned near the tip of 2HS distal from SSR marker Bmac0134. The
*brh4* locus was positioned near bin 9 of 2HL and *brh10* was position
in bins 4 or 5 of 2HS (Dahleen *et al*., 2005).

Hori *et al*. (2005) mapped QTLs for resistance Fusarium head blight
(FHB), incited primarily by *Fusarium graminearum*, using recombinant
inbred lines (RILs) from a cross between a resistant two-rowed accession
'Russian 6' and a very susceptible six-rowed accession H.E.S. 4 from
Afghanistan. Reactions to FHB were determined using a cut spike test
where field grown spikes were harvested at anthesis and sprayed with a
conidial suspension. The six-rowed spike 1 (*vrs1*) and closed flowering
(*cly1*/*Cly2*) loci were mapped on 2HL. Two QTLs for FHB severity were
detected on 2HL: one near the *vrs1* locus in bin 10 and one near the
*cly1/Cly2* locus in bin 13. Rachis internode length was correlated with
FHB severity. Other QTLs found on 2HL included early heading in bin 8,
plant height and number fertile rachis nodes (spike length) in bin 10,
and rachis internode length near bin 13.

Hori *et al*. (2006) used two-rowed barley accessions from China and
Turkey to map QTLs for resistance to FHB. A set of recombinant inbred
lines (RILs) was developed with 'Harbin' as the resistant parent and
'Turkey 6' as the susceptible parent. Using the cut spike to test FHB
reactions, QTLs for FHB severity were not detected in the bin 7 to 10
region of 2HL. This result suggests that these two-rowed parents were
homogeneous for QTLs controlling FHB severity in this region. A QTL for
FHB severity was detected on 2HL and positioned near (5.8 cM) the closed
flowering (*cly1*/*Cly2*) locus, probably in bin 13. Rachis internode
length was correlated with FHB severity in this study.

Horsley *et al*. (2006) reported that chromosome 2HL contains a series
of agronomically important traits and QTLs for resistance to FHB and for
the accumulation of the toxin deoxynivalenol (DON). 'Foster', a Midwest
six-rowed cultivar, was crossed to the resistant two-rowed accession
CIho 4196. RILs were evaluated in 10 field grown tests for FHB and in
several tests for DON accumulation and for morphological traits. QTLs
for various traits were found primarily on 2HL. QTLs for FHB severity
and DON level were in bins 8 and 10 and were named *Qrgz-2H-8* and
*Qrgz-2H-10*, respectively. These QTLs have been found in several other
studies where FHB resistance was evaluated in crosses between two- and
six-rowed cultivars. A QTL for DON was found in bin 2 of 4HS. A QTL for
early heading was found in bin 8 of 2HL and is presumably the *Eam6*
gene from the six-rowed parent. A QTL for low number of fertile rachis
nodes was located in bin 10 near the six-rowed spike 1 (*vrs1*) locus.
This QTL probably was identified earlier as the *lin1* locus. One or two
QTLs for plant height were also found very close to the *vrs1* locus.
Since the genes *Eam6*, *lin1*, and *vrs1* and the QTLs for
susceptibility to FHB and shortness were all contributed by the
six-rowed cultivar, breeding adapted lines with improved FHB resistance
has been difficult in six-rowed barley. QTLs for spike angle and spike
density or rachis internode length were located in bin 13 of 2HL. A
number of these associations on 2HL were previous reported by Dahleen
*et al*. (2003).

The transfer of favorable genes from wild barley to cultivated barley
was evaluated in backcross two of a doubled-haploid population by von
Korff *et al*. (2006). Early heading and short stature were associated
with the early maturity 1 (*Eam1* or *Ppd-H1*) gene in the bin 3 region
of 2HS. A second QTL for short stature was found in the bin 7 to 9
region of 2HL. A QTL for lodging resistance was found in bins 12 to 13
of 2HL.

Sameri and Komatsuda (2004) studied heading time in barley using RILs
from a cross between a winter six-rowed accession and a spring two-rowed
cultivar. Heading times for the RILs were estimated under long-day,
short-day, and continuous light conditions. Two QTLs for early heading
were detected on 2H under both spring and fall sown conditions, but not
under continuous light. The QTL near the centromere from the winter
parent, Azumamugi, probably corresponds to the *Eam6* or *eps2S* locus.
The QTL on 2HL was also from the winter parent, but at a position not
frequently associated with early maturity genes in barley.

Liu *et al*. (2005) identified in barley two full-length cDNA sequences
homologous to caleosin, a seed-storage oil-body protein from sesame. The
cDNAs, named *HvClo1* and *HvClo2*, are paralogs that cosegregate and
were mapped on chromosome 2HL in bin 9 near marker CDO588.. *HvClo1* is
expressed during late stages of embryogenesis and is seed specific.
*HvClo2* is expressed in endosperm tissues during grain development.

Tondell *et al*. (2006) observed that four of twelve drought tolerance
QTLs found on a barley consensus map were associated with regulatory
candidate genes that mapped in similar genome positions. One of the four
candidate genes is on chromosome 2HL in the bin 9 region.

Rostoks *et al*. (2005) used SNP discovery and linkage analysis to
construct an integrated SNP map of more than 300 SNP loci. With the
integration of RFLP, AFLP, and SSR markers, the map contained a total of
1,237 loci. Two regions of chromosome 2H were associated with QTLs for
seedling tolerance to high salt concentrations.

**References:**

Börner, A., V. Korzun, S. Malyshev, and V. Ivandic. 1999. Molecular
mapping of two dwarfing genes differing in their GA response on
chromosome 2H of barley. Theor. Appl. Genet. 99:670-675.

Dahleen, L.S., H.A. Agrama, R.D. Horsley, B.J. Steffenson, P.B. Schwarz,
A. Mesfin, and J.D. Franckowiak. 2003. Identification of QTLs associated
with Fusarium head blight resistance in Zhedar 2. Theor. Appl. Genet.
108:95-104.

Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005.
Characterization and molecular mapping of genes determining semidwarfism
in barley. J. Hered. 96:654-662.

Gottwald, S., N. Stein, A. Börner, T. Sasaki, and A. Graner. 2004. The
gibberellic-acid insensitive dwarfing gene *sdw3* of barley is located
on chromosome 2HS in a region that shows high colinearity with rice
chromosome 7L. Mol. Gen. Genomics 271:426-436.

Hori, K., T. Kobayashi, K. Sato, and K. Takeda. 2005. QTL analysis of
Fusarium head blight resistance using a high-density linkage map in
barley. Theor. Appl. Genet. 111:1661-1672.

Hori, K., K. Sato, T. Kobayashi, and K. Takeda. 2006. QTL analysis of
Fusarium head blight severity in recombinant inbred population derived
from a cross between two-rowed barley varieties. Breed. Sci. 56:25-30.

Horsley, R.D., D. Schmierer, C. Maier, D. Kudrna, C.A. Urrea, B.J.
Steffenson, P.B. Schwarz, J.D. Franckowiak, M.J. Green, B. Zhang, and A.
Kleinhofs. 2006. Identification of QTLs associated with Fusarium head
blight resistance in barley accession CIho 4196. Crop Sci. 46:145-156.

Korff, M. von, H. Wang, J. Léon, and K. Pilen. 2006. AB-QTL analysis in
spring barley: II. Detection of favourable exotic alleles for agronomic
traits introgressed from wild barley (*Hordeum vulgare* ssp.
*spontaneum*). Thoer. Appl. Genet. 112:1221-1231.

Lui H., P. Hedley, L. Cardle, K.M. Wright, I. Hein, D. Marshall, and R.
Waugh. 2005. Characterization and functional analysis of two barley
caleosins expressed during barley caryopsis development. Planta
221:513-532.

Rostoks, N., S. Mudie, L. Cardle, J. Russell, L. Ramsay, A. Booth, J. T.
Svensson, S.I. Wanamaker, H. Walia, E.M. Rodriguez, P.E. Hedley, H. Liu,
J. Morris, T,J. Close, D.F. Marshall, and R. Waugh. 2005. Genome-wide
SNP discovery and linkage analysis in barley based on genes responsive
to abiotic stress. Mol. Gen. Genomics 274:515-527.

Sameri, M. and T. Komatsuda. 2004. Identification of quantitative trait
loci (QTLs) controlling heading time in the population generated from a
cross between Oriental and Occidental barley cultivars (*Hordeum
vulgare* L.). Breed. Sci. 54:327-332.

Tondell, A. E. Francia, D. Barabschi, A. Aprile, J.S. Skinner, E.J.
Stockinger, A.M. Stanca, and N. Pecchioni. 2006. Mapping regulatory
genes as candidates for cold and drought stress tolerance in barley.
Theor. Appl. Genet. 112:445-454.

**Coordinator's Report: Barley Chromosome 3H**

L. Ramsay

Genetics Programme

Scottish Crop Research Institute

Invergowrie, Dundee, DD2 5DA, Scotland, UK.

e-mail: Luke.Ramsay@scri.ac.uk

Over the last year there have been a number of publications reporting
the mapping of genes and QTL on barley chromosome 3H. The largest number
of genes assigned to 3H was the 271 mapped by Cho *et al.* (2006) using
transcriptome analysis on the wheat-barley disomic chromosome addition
lines. Rostoks *et al*. (2005) mapped 51 genes to 3H as part of a
genome--wide SNP discovery programme in which over 300 genes responsive
to abiotic stress were mapped, mostly as SNPs. These publications
confirmed the close syntenic relationship between barley 3H and rice
chromosome 1. This synteny was used by Mammadov *et al*. (2005) to
direct the development of 9 EST-derived STS markers that were mapped
onto a high resolution map of the leaf rust resistance gene *Rph5*
region on 3HS including five that co-segregated with the resistance
gene. Hori *et al*. (2005) also published mapping data based on 60 EST
derived markers, 7 of which mapped to 3H. These represent a small subset
of 163 mapped to 3H by Sato *et al*. (2004), however primer information
on the seven is given in Hori *et al*. (2005) allowing the association
of EST sequences to the loci.

The mapping of individual genes has also reported in the last year with
the barley homologue of GIGANTEA, *HvGI*, mapping to a syntenic position
on 3HS (Bin 5-6) (Dunford *et al*., 2005). This gene is the homologue of
an *Arabidopsis* flowering time regulator, however its map position does
not correspond to the map position of any known flowering time QTL in
barley. Skinner *et al*. (2006) reported the mapping of *HvICE2* a
homologue of an Arabidopsis low temperature regulatory gene to 3HL (Bin
13-14). However, again, the map position of this candidate gene did not
correspond to the position of a known low-temperature tolerance QTL.

The barley homologue of *acsF,* an enzyme involved in chlorophyll
biosynthesis, was mapped to the short arm of chromosome 3H through the
use of the wheat-barley disomic chromosome addition lines and was shown
to be the known mutant *Xantha-l* (Rzeznicka *et al*.,. 2005).

Although much mapping work utilised the growing genomic resources in
barley there were reports that used more generic approaches. Thus
Mammadov *et al*. (2006) utilised degenerate primers designed to
conserved motifs of the NBS region in known resistance genes to isolate
190 resistance gene analogues (RGA) clones from barley genomic DNA and
mapped two of them to 3H (Bin 4 and Bin 14) using the Steptoe x Morex DH
mapping population. AFLP have been used for detailed mapping of the
*btr1/btr2* locus on 3HS (Senthil and Komatsuda, 2005) and some of these
have been converted to STS markers (Azhacuvel *et al*., 2006).

Again this year a considerable number of QTL were reported in the
literature some of which mapped to 3H. These included an increasing
number of reports using recombinant chromosome substation lines to
delineate association of quantitative traits with genomic regions (Hori
*et al*., 2005, von Korff *et al*., 2005, 2006, Yun *et al*., 2006).
Thus von Korff *et al*. (2005) report QTL for powdery mildew resistance
on 3HS (Bin 5-6) and on 3HL (Bin 13-15) with the latter interval also
housing QTL for resistance to leaf rust and scald. The same population,
derived from *H. vulgare spontaneum* introgressions into the spring
barley cultivar Scarlett, has also been assessed for agronomic traits
(von Korff *et al*., 2006). Several traits are reported to be associated
with regions on chromosome 3H including brittleness of the rachis with a
region on 3HS (Bin 3-6) and a large number of traits including height
and harvest index with a region on 3HL (Bin 10-16). The authors
postulate that these associations could be explained by the segregation
of *btr1* and *sdw-1* (denso) respectively in this population. Other
QTLs on 3H found in this study do not have obvious candidate genes but
are consistent with other studies. Thus a QTL for thousand grain weight
found on the distal end of 3HL (Bins 14-15) appears to relate to a
similar QTL found by Hori *et al*. (1995) in a doubled haploid
population derived from a cross between the cultivar Haruna Nijo and a
*Hordeum sponteneum* accession. Other QTL found on populations derived
from the same cross include ear length, number of spikelets and culm
length (Hori *et al*., 1995).

Other studies that report QTL on 3H include those for agronomic
characters discovered using the Steptoe x Morex mapping population
reported by Peighambari *et* *al*. (2005). The QTL found on 3H include
those for date of flowering, date of maturity, plant height and spike
length (Peighambari *et al*., 2005). In an extensive study on straw
quality characteristics reported by Grando *et al*. (2005) the QTL
reported on 3H include those for acid detergent fibre, lignin content,
voluntary intake and digestible organic matter (Grando *et al*., 2005).

In addition to the work reported in von Korff *et al*. (2005) other
disease resistance QTL have been reported on 3H in the last year. Bilgic
*et al*. (2005) found a total of four QTL for seedling (Bins 4-6 and
11-12) and adult resistance (Bins 2-4 and 9-11) to spot blotch in a
study comparing resistance expression in four populations. The authors
postulate that the seedling and adult resistances could relate to the
same underlying QTL and note that the resistance mapped to 3HS does not
correspond to anything reported previously (Bilgic *et al*., 2005). Yun
*et al*. (2005) report a net blotch QTL on 3H (Bin 6) shown in a RIL
population derived from a cross between H. *vulgare spontaneum* (OUH602)
and the cultivar Harrington. This QTL was confirmed in a RCSL population
derived from the same cross (Yun *et al*., 2006).

**References:**

Azhacuvel P., D. Vidya-Saraswathi, and T. Komatsuda. 2006.
High-resolution linkage mapping for the non-brittle rachis locus *btr1*
in cultivated x wild barley (*Hordeum vulgare*) Plant Sci 170:
1087-1094.

[Bilgic
H](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Bilgic+H&curr_doc=4/1&Form=FullRecordPage&doc=4/1),
B.
[Steffenson](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Steffenson+B&curr_doc=4/1&Form=FullRecordPage&doc=4/1),
and P.
[Hayes.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Hayes+P&curr_doc=4/1&Form=FullRecordPage&doc=4/1)
2005. Comprehensive genetic analyses reveal differential expression of
spot blotch resistance in four populations of barley. Theor Appl Genet
111: 1238-1250.

Cho, S.H., D.F. Garvin, and G.J. Muehlbauer. 2006. Transcriptome
analysis and physical mapping of barley genes in wheat-barley chromosome
addition lines. Genetics 172: 1277-1285.

Dunford, RP., S. Griffiths, V. Christodoulou, and D.A. Laurie. 2005.
Characterisation of a barley (*Hordeum vulgare* L.) homologue of the
*Arabidopsis* flowering time regulator *GIGANTEA*. Theor Appl Genet 110:
925-931.

Grando S, M.
[Baum](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Baum+M&curr_doc=12/5&Form=FullRecordPage&doc=12/5),
S.
[Ceccarelli](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Ceccarelli+S&curr_doc=12/5&Form=FullRecordPage&doc=12/5),
A.
[Goodchild,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Goodchild+A&curr_doc=12/5&Form=FullRecordPage&doc=12/5)
F.J.
[El-Haramein](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=El-Haramein+FJ&curr_doc=12/5&Form=FullRecordPage&doc=12/5),
A.
[Jahoor,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Jahoor+A&curr_doc=12/5&Form=FullRecordPage&doc=12/5)
and G.
[Backes.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Backes+G&curr_doc=12/5&Form=FullRecordPage&doc=12/5)
2005. QTLs for straw quality characteristics identified in recombinant
inbred lines of a *Hordeum vulga*re x *H spontaneum* cross in a
Mediterranean environment  Theor Appl Genet 110 : 688-695

Hori, K., K. Sato, N. Nankaku, and K. Takeda. 2005. QTL analysis in
recombinant chromosome substitution lines and doubled haploid lines
derived from a cross between *Hordeum vulgare* ssp *vulgare* and
*Hordeum vulgare* ssp *spontaneum*. Mol Breeding 16:295-311.

Korff M. von, H. Wang, J. Léon, and K. Pillen K.. 2005. AB-QTL analysis
in spring barley. I. Detection of resistance genes against powdery
mildew, leaf rust and scald introgressed from wild barley. Theor Appl
Genet 111: 583-590.

Korff M. von, H. Wang, J. Léon, and K. Pillen. 2006. AB-QTL analysis in
spring barley. II. Detection of favourable exotic alleles for agronomic
traits introgressed from wild barley. Theor Appl Genet 112: 1221-1231.

Mammadov, J.A., Z. Liu, R.M. Biyashev, G.J. Muehlbauer, and M.A.S.
Maroof.. 2006. Cloning, genetic and physical mapping of resistance gene
analogs in barley (*Hordeum vulgare* L.). Plant Breeding 125: 32-42.

Mammadov, J.A., B.J. Steffenson, and M.A.S. Maroof. 2005.
High-resolution mapping of the barley leaf rust resistance gene *Rph5*
using barley expressed sequence tags (ESTs) and synteny with rice. Theor
Appl Genet 111: 1651-1660.

Peighambari, S.A., B.Y. Samadi, A. Nabipour, G. Charmet and A. Sarrafi.
2005. QTL analysis for agronomic traits in a barley doubled haploids
population grown in Iran. Plant Sci 169: 1008-1013.

Rostoks, N., S. Mudie, L. Cardle, J. Russell, L. Ramsay, A. Booth, J.T.
Svensson, S.I. Wanamaker, H. Walia, E.M. Rodriguez, P.E. Hedley, H. Liu,
J. Morris, T.J. Close, D.F. Marshall, and R. Waugh. 2005. Genome-wide
SNP discovery and linkage analysis in barley based on genes responsive
to abiotic stress. Mol Gen Genom 274: 515-527.

Rzeznicka, K., C.J. Walker, T. Westergren, C.G. Kannangara, D. von
Wettstein, S. Merchant, S.P. Gough and M.Hansson. 2005. *Xantha-l*
encodes a membrane subunit of the aerobic Mg-protoporphyrin IX
monomethyl ester cyclase involved in chlorophyll biosynthesis. Proc Nat
Acad U.S.A. 102: 5886-5891.

Sato, K., N. Nankaku, Y. Motoi, and K. Takeda. 2004. A large scale
mapping of ESTs on barley genome. *In*: J. Spunar and J, Janikova
(eds.), pp. 79-85. Barley Genetics IX. Proc.Ninth Int. Barley Genet.
Symp., Brno, Czech Republic, June 20-26 2004.

Senthil, N. and T. Komatsuda. 2005. Inter-subspecific maps of
non-brittle rachis genes *btr1/btr2* using occidental, oriental and wild
barley lines. Euphytica 145: 215-220.

[Skinner
J](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Skinner+J&curr_doc=3/2&Form=FullRecordPage&doc=3/2).,
P.
[Szűcs](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Szucs+P&curr_doc=3/2&Form=FullRecordPage&doc=3/2),
J. [von Zitzewitz,
L.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=von+Zitzewitz+J&curr_doc=3/2&Form=FullRecordPage&doc=3/2)
[Marquez-Cedillo](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Marquez-Cedillo+L&curr_doc=3/2&Form=FullRecordPage&doc=3/2),
T.
[Filichkin,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Filichkin+T&curr_doc=3/2&Form=FullRecordPage&doc=3/2)
E.J,
[Stockinger,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Stockinger+EJ&curr_doc=3/2&Form=FullRecordPage&doc=3/2)
M.F.
[Thomashow,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Thomashow+MF&curr_doc=3/2&Form=FullRecordPage&doc=3/2)
T.H.H. [Chen, and
P.M.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Chen+THH&curr_doc=3/2&Form=FullRecordPage&doc=3/2)
[Hayes](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Hayes+PM&curr_doc=3/2&Form=FullRecordPage&doc=3/2).
2006. Mapping of barley homologs to genes that regulate low temperature
tolerance in *Arabidopsis*. Theor Appl Genet 112: 832-842.

[Yun
S.J](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Yun+SJ&curr_doc=5/3&Form=FullRecordPage&doc=5/3),
L. [Gyenis,
P.M.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Gyenis+L&curr_doc=5/3&Form=FullRecordPage&doc=5/3)
Hayes, I.
[Matus](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Matus+I&curr_doc=5/3&Form=FullRecordPage&doc=5/3),
K.P.
[Smith](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Smith+KP&curr_doc=5/3&Form=FullRecordPage&doc=5/3),
B.J. [Steffenson,
and](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Steffenson+BJ&curr_doc=5/3&Form=FullRecordPage&doc=5/3)
G.J.
[Muehlbauer.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Muehlbauer+GJ&curr_doc=5/3&Form=FullRecordPage&doc=5/3)
2005. Quantitative trait loci for multiple disease resistance in wild
barley. Crop Sci 45: 2563-2572.

[Yun
S.J](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Yun+SJ&curr_doc=5/1&Form=FullRecordPage&doc=5/1),
L.
[Gyenis,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Gyenis+L&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
E.
[Bossolini,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Bossolini+E&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
P.M. Hayes, I.
[Matus,](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Matus+I&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
K.P.
[Smith](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Smith+KP&curr_doc=5/1&Form=FullRecordPage&doc=5/1),
B.J. [Steffenson,
R.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Steffenson+BJ&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
[Tuberosa, and
G.J.](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Tuberosa+R&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
[Muehlbauer](http://apps.isiknowledge.com/WoS/CIW.cgi?SID=X1Of2KhJ8cl1LhKAG5l&Func=OneClickSearch&field=AU&val=Muehlbauer+GJ&curr_doc=5/1&Form=FullRecordPage&doc=5/1)
2006. Validation of quantitative trait loci for multiple disease
resistance in barley using advanced backcross lines developed with a
wild barley. Crop Sci 46: 1179-1186.

**Coordinators Report: Chromosome 5H(7)**

George Fedak

Eastern Cereal & Oilseed Research Centre

Agriculture & Agri-Food Canada

Ottawa, Ontario, K1A 0C6

e-mail: fedakga@agr.gc.ca

Winterhardiness in winter barley is controlled by regulatory elements of
photoperiod sensitivity and vernalization response combined with the
physical trait of low temperature tolerance. Of the six photoreceptors
mapped on two mapping populations, only one, *HvPhyC*, coincided with a
photoperiod response QLT on chromosome 5HL (Szucs *et al*., 2006). The
vernalization locus *VRN-H1* (*HvBM5A*) whose expression is regulated by
photoperiod has been mapped on chromosome 5HL and is closely linked to
*HvPhyC*.

Reproductive frost tolerance is the ability of reproductive organs to
tolerate low temperatures. A QTL on chromosome 5H for tolerance to
frost-induced floret sterility and frost-induced grain damage was
identified in three mapping populations (Reinheimer *et al.*, 2004).
This locus is located close to the vrn-H1 locus on chromosome 5H and has
been associated with the locus giving a response at both vegetative and
reproductive developmental stages.

Seed dormancy is an important trait that can prevent preharvest
sprouting and regulate germination during the malting process. A major
seed dormancy QLT was detected on chromosome 5H plus two others on
chromosome 1H in a mapping population derived from crossing the Japanese
malting cultivar Haruna Nijo x H602 (*H. spontaneum -- dormant*) (Sato
*et al*., 2006). Seven EST markers were localized in the vicinity of the
QLT on chromosome 5H.

Identification of QTL resistance to Fusarium Head Blight (FHB) continues
to be a challenging exercise. Chromosome 5H appears to be a lesser
contributor of FHB resistance QTL. For example, in recombinant inbred
populations derived from two-rowed crosses of Harbin (R) x Turkey 6
(HR), resistance QTL were located on all chromosomes except 5H (Hori *et
al*., 2006). However, in an RI population derived from Russia 6 (HR) x
HES4 (HS), which was mapped with 1,255 markers, two pulative resistance
loci were located on chromosome 2H and one on 5H (Takeda, 2004).

Of more general interest to barley geneticists are the assembly of a
high density microsatellite consensus map and the sequencing of the
barley chloroplast genome. The consensus microsatellite or SSR map was
assembled by combining the information from six independent mapping
populations. It consists of 784 unique microsatellite loci from 696
primers spanning 1,137.6 cM with an average density of one SSR marker
every 1.45 cM (Varshney *et al*., 2006).

The chloroplast genome of barley consists of 136,462 bp, including a
large single copy region of 80,600 bp, a small single copy region of
12,704 bp, plus a pair of inverted repeats of 21,597 bp. The genome
consists of 104 genes, including 70 peptide-encoding genes, plus 30 tRNA
and 4 rRNA genes that are duplicated in the inverted repeat (Saski et
al., 2006). This genome is practically identical to other cereal
chloroplast genomes, indicating that such genomes are highly conserved.

**References:**

Hori, K., K. Sato, and K. Takeda. 2006. Comparison of Fusarium heat
blight resistance loci in barley RI populations. Poster 327. Plant and
Animal Genome XIV Conference.
(<http://www.intl-pag.org/14/abstracts/PAG14_P327.htm>[)]{.underline}.

Reinheimer, J.L., A.R. Bar, and J.K. Eglinton. 2004. QTL mapping of
chromosomal regions conferring reproductive frost tolerance in barley
(*Hordeum vulgareL.*). Theor. Appl. Genet. 109:1267-1274.

Saski, C.A., S.B. Lee, H. Daniell, and J.P. Tomkins. 2006. The complete
DNA sequence of the sorghum and barley chloroplast genomes. Poster 235.
Plant and Animal Genome XIV Conference.

> (<http://www.intl-pag.org/14/abstracts/PAG14_P327.htm>[).]{.underline}

Sato, K., K. Hori, and K. Takeda. 2006. QTL for seed dormancy from wild
barley *Hordeum Vulgare ssp. Spontaneum*. Poster 309. Plant and Animal
Genomes XIV Conference.
(<http://www.intl-pag.org/14/abstracts/PAG14_P327.htm>[).]{.underline}

Szucs, P., I. Karsai, J. von Zitzewitz, L.D.D. Cooper, T.H.H. Chen, P.M.
Hayes, and J.S. Skinner. 2006. Positional relationship between
photoperiod response QTL and photoreceptor and vernalization genes in
barley. Poster 351. Plant and Animal Genomes XIV Conference.

> (<http://www.intl-pag.org/14/abstracts/PAG14_P327.htm>[).]{.underline}

Takeda, K. 2004. Inheritance of the Fusarium Head Blight resistance in
barley. Czech J. Genet. Plant Breed. 40:143.

Varshney, R.K., T.C. Marcell, L. Ramsey, J. Russell, M.S. Roeder, N.
Stein, P. Langridge, R. Waugh, R. Niks, and A. Graner. 2006. A high
density microsatellite consensus map of barley. Workshop 304. Plant and
Animal Genome XIV Conference.

> (<http://www.intl-pag.org/14/abstracts/PAG14_P327.htm>[).]{.underline}

**Coordinator's Report: Chromosome 7H**

Lynn S. Dahleen.

USDA-Agricultural Research Service

Fargo, ND 58105, USA

e-mail: DAHLEENL@fargo.ars.usda.gov

2005 brought many reports of various QTLs detected in populations
derived from wild x cultivated barley crosses, with the goal of
transferring desirable genes into elite breeding lines. Hori *et al.*
(2005b) located QTLs for glume length, rachis-internode length, dormancy
after five and ten weeks, ear length and kernel weight on chromosome 7H
in a population derived from a cross with *H. vulgare* ssp. *spontaneum*
(accession H602). An examination of straw quality QTLs (Grando *et al*.
2005) located several loci on chromosome 7H, for traits acid detergent
fiber, lignin content, voluntary intake, and percentage of sheaths by
weight of the air-dried straw sample. They used a RIL population derived
from *H. vulgare* ssp. *spontaneum* accession 41-1. Li *et al*. (2005)
determined QTLs for yield, yield components and malting quality in an
advanced backcross population with *H. vulgare* ssp. *spontaneum*
accession HS213. Five QTLs were located on chromosome 7H, for heading
date, ear length, spikelet number per spike, protein content and
friability. QTLs involved in dormancy and desiccation tolerance were
located in *H. vulgare* ssp. *spontaneum* accession Wadi Qilt genotype
23-39 (Zhang *et al*. 2005a). Loci for maximum germination rate under
drought stress, and minimum and maximum revival after drought stress
were located on chromosome 7H. A new dominant scald resistance gene,
*Rrs15* derived from *H. vulgare* ssp. *spontaneum* (accession CPI 77132
Caesarea plant 38), was located on the long arm of chromosome 7H, near
the SSR marker HVM49 (Genger *et al*. 2005). In another study using *H.
vulgare* ssp. *spontaneum* (accession OUH602), Yun *et al.* (2005)
identified a new resistance locus on chromosome 7H for spot blotch
(*Rcs2-4*). This gene was located on the short arm of the chromosome in
a cluster of genes for resistance to fungal diseases. A third disease
resistance study with *H. vulgare* ssp. *spontaneum* (accession ISR42-8)
used advanced backcross QTL analysis and located two resistance loci on
chromosome 7H, one for powdery mildew (QPm.S42-7H.a) and one for leaf
rust (QLr.S42-7H.a), both on the long arm (von Korff *et al*. 2005).

Additional studies located genes and QTLs from cultivated crosses.
Emebiri *et al*. (2005b) examined disease resistance in a two-rowed
barley population segregating for malting quality traits. The only locus
on chromosome 7H, identified by QTL and classical linkage analyses, was
for stem rust resistance, likely *Rpg1*. Adult and seedling resistance
to spot blotch in Morex was compared in four doubled haploid populations
by Bilgic *et al*. (2005). They found that the locus on chromosome 7H,
presumably *Rcs5*, was consistently identified for both seedling and
adult plant resistance, while loci on other chromosomes were not found
in all four populations. Hori *et al*., (2005a) located QTLs for
Fusarium head blight resistance from the cultivar Russia 6, along with
QTLs for spike morphology. They located QTLs for rachis-internode length
and heading date on chromosome 7H.

In a cross between two low protein parents, Emebiri *et al*. (2005a)
located QTLs for grain protein content on five chromosomes. The one on
chromosome 7H significantly reduced protein in six of the eight
environments tested and was not associated with QTLs for yield, height
or heading date. Peighambari *et al*. (2005) tested the Steptoe x Morex
doubled haploid population for agronomic traits in Iran. Only two QTLs
were located on chromosome 7H, for date of spike initiation and 1000
seed weight. Dahleen *et al*. (2005) characterized and located genes for
27 brachytic semidwarf mutants using SSR markers on near-isogenic lines.
One of the new mutants, *brh.v*, was located on chromosome 7H, and the
*brh1.z* allele mapped to the expected location of the previously mapped
*brh1* locus.

One study has looked at expanding our selection of molecular markers for
barley. Zhang *et al*. (2005b) tested 98 EST-SSR markers derived from
wheat sequences in barley. They found that 50.4% of the markers
amplified sequences in barley. When they examined some of the amplified
sequences in more detail, most had repeats similar to those in wheat.

Additional mapping and marker work can be found in proceedings from
various meetings, like the North American Barley Researchers Workshop,
held in Red Deer Alberta last July.

**References:**

Bilgic, H., B.J. Steffenson, and P.M. Hayes. 2005. Comprehensive genetic
analyses reveal differential expression of spot blotch resistance in
four populations of barley. Theor. Appl. Genet. 111:1238-1250.

Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005.
Characterization and molecular mapping of genes determining semidwarfism
in barley. J. Hered. 96:654-662.

Emebiri, L.C., D.B. Moody, R. Horsley, J. Panozzo, and B.J. Read. 2005a.
The genetic control of grain protein content variation in a doubled
haploid population derived from a cross between Australian and North
American two-rowed barley lines. J. Cereal Sci. 41:107-114.

Emebiri, L.C., G. Platz, and D.B. Moody. 2005b. Disease resistance genes
in a doubled haploid population of two-rowed barley segregating for
malting quality attributes. Australian J. Agric. Res. 56:49-56.

Genger, R.K., K. Nesbitt, A.H.D. Brown, D.C. Abbott, and J.J. Burdon.
2005. A novel barley scald resistance gene: genetic mapping of the
*Rrs15* scald resistance gene derived from wild barley, *Hordeum
vulgare* ssp. *spontaneum*. Plant Breeding 124:137-141.

Grando, S., M. Baum, S. Ceccarelli, A. Goodchild, F. Jaby El-Haramein,
A. Jahoor, and G. Backes. 2005. QTLs for straw quality characteristics
identified in recombinant inbred lines of a *Hordeum vulgare* x *H.
spontaneum* cross in a Mediterranean environment. Theor. Appl. Genet.
110:688-695.

Hori, K., T. Kobayashi, K. Sato, and K. Takeda. 2005a. QTL analysis of
Fusarium head blight resistance using a high-density linkage map of
barley. Theor. Appl. Genet. 111:1661-1672.

Hori, K., K. Sato, N. Nankaku, and K. Takeda. 2005b. QTL analysis in
recombinant chromosome substitution lines and doubled haploid lines
derived from a cross between *Hordeum vulgare* ssp. *vulgare* and
*Hordeum vulgare* ssp. *spontaneum*. Molec. Breeding 16:295-311.

Korff, M. von, H. Wang, J. Léon, and K. Pillen. 2005. AB-QTL analysis in
spring barley. 1. Detection of resistance genes against powdery mildew,
leaf rust and scald introgressed from wild barley. Theor. Appl. Genet.
111:583-590.

Li, J.Z., X.Q. Huang, F. Heinrichs, M.W. Ganal, and M.S. Röder. 2005.
Analysis of QTLs for yield, yield components, and malting quality in a
BC~3~-DH population of spring barley. Theor. Appl. Genet. 110:356-363.

Peighambari, S.A., B.Y. Samadi, A. Nabipour, G. Charmet, and A. Sarrafi.
2005. QTL analysis for agronomic traits in a barley doubled haploids
population grown in Iran. Plant Sci. 169:1008-1013.

Yun, S.J., L. Gyenis, P.M. Hayes, I. Matus, K.P. Smith, B.J. Steffenson,
and G.J. Muehlbauer. 2005. Quantitative trait loci for multiple disease
resistance in wild barley. Crop Sci. 45:2563-2572.

Zhang, F., G. Chen, Q. Huang, O. Orion, T. Krugman, T. Fahima, A.B.
Korol, E. Nevo, and Y. Gutterman. 2005a. Genetic basis of barley
caryopsis dormancy and seedling desiccation tolerance at the germination
stage. Theor. Appl. Genet. 110:445-453.

Zhang, L.Y., M. Bernard, P. Leroy, C. Feuillet, and P. Sourdille. 2005b.
High transferability of bread wheat EST-derived SSRs to other cereals.
Theor. Appl. Genet. 111:677-687.

**Integrating Molecular and Morphological/Physiological Marker Maps**

A. Kleinhofs

## Dept. Crop and Soil Sciences and

# School of Molecular Biosciences

Washington State University

Pullman, WA 99164-6420, USA

e-mail: andyk@wsu.edu

Updates to barley morphological/physiological genetic map and gene
cloning include publication of the cloning and characterization of the
barley *Nec1* locus encoding a cyclic nucleotide-gated ion channel gene
(Rosto*ks et al*. 2006). The previously reported *rym4* locus coding for
the eukaryotic translation initiation factor 4E has been published
(Kanyu*ka et al*. 2005).

The barley spring vs. winter growth habit candidate genes were cloned
and characterized (Von Zitzewi*tz et al*. 2005). The *sgh1* locus,
renamed *Vrn-H2* to conform with the wheat nomenclature, maps to
chromosome 4 (4H) bin 12 approximately 8 cM proximal to *Bmy1* and
co-segregating with the *HvSnf2* gene. The *sgh1 (Vrn-H2)* locus,
represented by the *ZCCT-H* gene cluster, encodes a dominant
transcription factor flowering repressor. Accession numbers of the two
closely related candidate genes are AY485977 (*ZCCT-Ha*) and AY485978
(*ZCCT-Hb*). The *Sgh2* locus, renamed *Vrn-H1* to conform to the wheat
nomenclature is located on chromosome 7 (5H) bin 11 between markers
*Dhn2* and BCD265C (unfortunately designated BCD265B in Zitzewitz *et
al*.). However, the gene, designated *HvBM5A*, has been cloned and the
sequence is available (AY750995 genomic and AY785826 cDNA cv. Morex
sequences, respectively). *HvBM5A* encodes a MADS-box transcription
factor. A closely related gene, *HvBM5B*, maps to chromosome 5 (1H) bin
07 closely linked to ABG452. It is proposed that *HvBM5B* represents
*Sgh3*, renamed *Vrn-H3* in the wheat nomenclature.

The *Vrs1* gene has been cloned (Komatsuda, Plant & Animal Genome XIV,
Abstract W13 p10). Although details have not yet been published, the
*Vrs1* locus was reported to encode a homeobox gene.

The cloned morphological/physiological genes represent excellent anchor
points for the morphological/physiological barley map since the genes
themselves can be used as reference points in mapping populations. There
should be ample future opportunities for the identification of other
barley genes by homology to the model dicot and monocot plants.

The leaf rust resistance gene *Rph5* was mapped at a high resolution and
shown to co-segregate with ABG070 and five ESTs (Mammad*ov et al*.
2005). *Rph5* was mapped previously, but this publication provides a
high-resolution map and many different closely linked molecular markers.

The location of the non-brittle rachis genes btr1/btr2 was further
refined (Senthil and Komatsuda, 2005). However, all of the new markers
are AFLP and difficult to integrate with the morphological map.

Molecular mapping located 18 brachytic (*brh*) loci to five of the seven
barley chromosomes, albeit with low resolution (Dahle*en et al*. 2005).
(The nomenclature of the new loci used here is that proposed by Dahleen
*et al*. for the actual alleles used for mapping see the original
paper). The *brh1* and locus was previously mapped with high resolution
to chromosome 1 (7H) bin01 and *brh2* was mapped to chromosome 4 (4H)
bin 05. Other loci mapping on chromosome 4 (4H) were *brh5* and *brh9*,
but lack of flanking markers makes it difficult to determine their bin
locations. The same problem exists for *brh3*, *brh4*, and *brh10* loci
mapped to chromosome 2 (2H), however *brh3* probably is in bin01. The
loci *brh8* and *brh14* were mapped to chromosome 3(3H). A large number
of loci were mapped to the short arm of chromosome 7 (5H) including
*brh6, brh7, brh11, brh12, brh13, brh17, brh18*. The locus *brh16* was
mapped on the long arm of chromosome 1 (7H). Although the sparse markers
and lack of flanking markers makes it impossible to reliably place these
loci in chromosome bins, they do provide a starting point for those
wishing to map these genes more precisely.

The *H*. *spontaneum* derived leaf scald resistance gene *Rrs15* was
mapped to chromosome 1 (7H) long arm 11,5 cM from HVM49 (Geng*er et al*.
2005). Since HVM49 is located in bin 12 and the direction of the linkage
was not indicated, *Rrs15* could be in bin 11 or 13. The isozyme marker
*Acp2* was linked to *Rrs15* at 17.7 cM.

The barley cytoplasmic male sterility restorer gene *Rfm1* was mapped to
chromosome 6 (6H) short arm (Muraka*mi et al*. 2005). Closely linked
AFLP markers were identified, however I was not able to assign the locus
to a bin.

A very clever use of rice synteny and Arabidopsis was used to identify a
cellulose synthase-like (*CslF*) gene cluster as candidates responsible
for mediating the cell wall (1,3;1,4)-B-D-glucan syntesis (Burt*on et
al*. 2006). The work was initiated from the map location of a major QTL
for (1,3;1,4)-B-D-glucan content of un-germinated barley grains on
chromosome 2 (2H). This QTL is located between the markers *Adh8* bin 6
and ABG019 bin 7 with the peak closer to ABG019. Therefore, I have
assigned the *CslF* locus to 2 (2H) bin 7. I believe this is the first
example of a map-based cloning of a QTL in barley.

Please advise me of any additions or corrections to this information.

> Bin Assignments for Morphological Map Markers and closest molecular
> marker
>
> Chr.1 (7H)
>
> BIN1 ABG704
>
> \*[Rpg1]{.mark} RSB228 Brueggeman *et al*., PNAS 99:9328, '02
>
> \*Run1
>
> [Rdg2a]{.mark} MWG851A Bulgarelli *et al*., TAG 108:1401, '04
>
> [Rrs2]{.mark} MWG555A Schweizer *et al*., TAG 90:920, '95
>
> mlt
>
> [brh1]{.mark} MWG2074B Li et al., 8th IBGS 3:72, '00
>
> BIN2 ABG320
>
> Est5 iEst5 Kleinhofs *et al*., TAG 86:705, '93
>
> [fch12]{.mark} BCD130 Schmierer *et al*., BGN 31:12, '01
>
> \*[wax]{.mark} Wax Kleinhofs BGN 32:152, '02
>
> [gsh3]{.mark} His3A Kleinhofs BGN 32:152, '02
>
> BIN3 ABC151A
>
> [fch5]{.mark} ABC167A Kleinhofs BGN 32:152, '02
>
> [Rcs5]{.mark} KAJ185 Johnson & Kleinhofs, unpublished
>
> yvs2
>
> [cer-ze]{.mark} ABG380 Kleinhofs BGN 27:105, '96
>
> BIN4 ABG380
>
> wnd
>
> [Lga]{.mark} BE193581 Johnson & Kleinhofs, unpublished
>
> abo7
>
> BIN5 ksuA1A
>
> ant1
>
> [nar3]{.mark} MWG836 Kleinhofs BGN 32:152, '02
>
> ert-m
>
> ert-a
>
> BIN6 ABC255
>
> ert-d
>
> fch8
>
> fst3
>
> cer-f
>
> msg14
>
> BIN7 ABG701
>
> dsp1 cMWG704 Sameri (in press)
>
> msg10
>
> [rsm1]{.mark} ABC455 Edwards & Steffenson, Phytopath. 86:184,'96
>
> sex6
>
> seg5
>
> seg2
>
> [pmr]{.mark} ABC308 Kleinhofs BGN 27:105, '96
>
> [mo6b]{.mark} Hsp17 Soule *et al*., J Her. 91:483, '00
>
> [nud]{.mark} CDO673 Heun *et al*., Genome 34:437, '91
>
> [fch4]{.mark} MWG003 Kleinhofs BGN 27:105, '96
>
> BIN8 \*[Amy2]{.mark} Amy2 Kleinhofs *et al*., TAG 86:705, '93
>
> [lks2]{.mark} WG380B Costa *et al*., TAG 103:415, '01
>
> [Rpt4]{.mark} Psr117D Williams *et al*., TAG 99:323, '99
>
> ubs4
>
> blx2
>
> BIN9 RZ242
>
> lbi3
>
> xnt4
>
> [lpa2]{.mark} ? Larson *et al*., TAG 97:141, '98
>
> msg50
>
> Rym2
>
> seg4
>
> BIN10 ABC310B
>
> [Xnt1]{.mark} BF626025 Hansson et *al*., PNAS 96:1744, '99
>
> [xnt-h]{.mark} BF626025 Hansson et *al*., PNAS 96:1744, '99
>
> BIN11 ABC305
>
> [Rph3]{.mark}
>
> Tha2 Toojinda *et al*., TAG 101:580, '00
>
> BIN12 ABG461A
>
> Mlf
>
> xnt9
>
> seg1
>
> msg23
>
> BIN13 Tha
>
> Rph19 Rlch4(Nc) Park & Karakousis Plt. Breed. 121:232. '02
>
> Chr.2 (2H)
>
> BIN1 MWG844A
>
> sbk
>
> brh3 Bmac0134 Dahleen *et al*., J. Heredity 96:654, '05
>
> BIN2 ABG703B
>
> BIN3 MWG878A [gsh6]{.mark} Kleinhofs BGN 32:152, '02
>
> gsh1
>
> gsh8
>
> BIN4 ABG318
>
> Eam1
>
> [Ppd-H1]{.mark} MWG858 Laurie *et al*., Heredity 72:619, '94
>
> sld2
>
> rtt
>
> flo-c
>
> sld4
>
> BIN5 ABG358
>
> fch15
>
> brc1
>
> com2
>
> BIN6 Pox
>
> msg9
>
> abo2
>
> Rph15 P13M40 Weerasena *et al*., TAG 108:712 '04
>
> [rph16]{.mark} MWG874 Drescher *et al*., 8thIBGS II:95, '00
>
> BIN7 Bgq60
>
> [yst4]{.mark} CDO537 Kleinhofs BGN 32:152, '02
>
> [Az94]{.mark} CDO537 Kleinhofs BGN 32:152, '02
>
> [gai]{.mark} MWG2058 Börner *et al*., TAG 99:670, '99
>
> msg33
>
> [\*HvCslF]{.mark} (barley Cellulose synthase-like) Burton *et al*.,
> Science 311:1940 '06
>
> [\*Bmy2]{.mark}
>
> msg3
>
> fch1
>
> BIN8 ABC468
>
> [Eam6]{.mark} ABC167b Tohno-oka et al., 8thIBGS III:239, '00
>
> gsh5
>
> msg2
>
> [eog]{.mark} ABC451 Kleinhofs BGN 27:105, '96
>
> abr
>
> cer-n
>
> BIN9 ABC451
>
> Gth
>
> hcm1
>
> wst4
>
> \*[vrs1]{.mark} MWG699 Komatsuda *et al*., Genome 42:248, '00
>
> BIN10 MWG865
>
> cer-g
>
> Lks1
>
> mtt4
>
> Pre2
>
> msg27
>
> BIN11 MWG503
>
> [Rha2]{.mark} AWBMA21 Kretschmer *et al*., TAG 94:1060, '97
>
> Ant2 MWG087 Freialdenhoven *et al*., Plt. Cell 6:983, '94
>
> \*[Rar1]{.mark} AW983293B Freialdenhoven et al., Plt. Cell 6:983, '94
>
> fol-a
>
> [gal]{.mark} MWG581A Börner *et al*., TAG 99:670, '99
>
> fch14
>
> Pau
>
> BIN12 ksuD22
>
> Pvc
>
> BIN13 ABC252
>
> [lig]{.mark} BCD266 Pratchett & Laurie Hereditas 120:35, '94
>
> [nar4]{.mark} Gln2 Kleinhofs BGN 27:105, '96
>
> [Zeo1]{.mark} cnx1 Costa *et al*., TAG 103:415, '01
>
> [lpa1]{.mark} ABC157 Larson *et al*., TAG 97:141, '98
>
> BIN14 ABC165
>
> BIN15 MWG844B
>
> [gpa]{.mark} CDO036 Kleinhofs BGN 27:105, '96
>
> [wst7]{.mark} MWG949A Costa *et al*., TAG 103:415, '01
>
> [MlLa]{.mark} Ris16 Giese *et al*., TAG 85:897, '93
>
> trp
>
> Chr. 3 (3H)
>
> BIN1 [Rph5]{.mark} ABG070 Mammadov *et al*., TAG 111:1651, '05
>
> [Rph6]{.mark} BCD907 Zhong *et al*., Phytopath. 93:604, '03
>
> [Rph7]{.mark} MWG848 Brunner *et al*., TAG 101:783, '00
>
> BIN2 JS195F BI958652; BF631357; BG369659
>
> ant17
>
> sld5
>
> [mo7a]{.mark} ABC171A Soule *et al*., J. Hered. 91:483, '00
>
> brh8
>
> BIN3 ABG321
>
> xnt6
>
> BIN4 MWG798B
>
> [btr1]{.mark} Senthil & Komatsuda Euphytica 145:215, '05
>
> [btr2]{.mark} Senthil & Komatsuda Euphytica 145:215, '05
>
> lzd
>
> [alm]{.mark} ABG471 Kleinhofs BGN 27:105, '96
>
> BIN5 BCD1532
>
> abo9
>
> sca
>
> yst2
>
> dsp10
>
> BIN6 ABG396
>
> [Rrs1]{.mark} Graner *et al*., TAG 93: 421 ´96
>
> [Rh/Pt]{.mark} ABG396 Smilde *et al*., 8th IBGS 2:178, '00
>
> [Rrs.B87]{.mark} BCD828 Williams *et al*., Plant Breed. 120:301, '01
>
> AtpbB
>
> abo6
>
> xnt3
>
> msg5
>
> ari-a
>
> yst1
>
> zeb1
>
> ert-c
>
> ert-ii
>
> cer-zd
>
> [Ryd2]{.mark} WG889B Collins *et al*., TAG 92:858, '96
>
> \*[uzu]{.mark} AB088206 Saisho *et al*., Breeding Sci. 54:409, '04
>
> BIN7 MWG571B
>
> cer-r
>
> BIN8 ABG377
>
> wst6
>
> cer-zn
>
> sld1
>
> BIN9 ABG453
>
> wst1
>
> BIN10 CDO345
>
> vrs4
>
> Int1
>
> gsh2
>
> BIN11 CDO113B
>
> als
>
> [sdw1]{.mark} PSR170 Laurie *et al*., Plant Breed. 111:198, '93
>
> BIN12 His4B
>
> sdw2
>
> BIN13 ABG004
>
> [Pub]{.mark} ABG389 Kleinhofs *et al*., TAG 86:705, '93
>
> BIN14 ABC161
>
> cur2
>
> BIN15 ABC174
>
> Rph10
>
> fch2
>
> BIN16 ABC166
>
> eam10
>
> Est1/2/3
>
> \*[rym4]{.mark} *eIF4E* Stein *et al*.,Plt. J. 42:912, '05

[\*rym5]{.mark}*eIF4E*and Kanyuka *et al*., Mol. Plant Path. 6:449, '05

> Est4
>
> ant28
>
> Chr.4 (4H)
>
> BIN1 MWG634
>
> BIN2 JS103.3
>
> fch9
>
> sln
>
> BIN3 Ole1 [Dwf2]{.mark} Ivandic *et al*., TAG 98:728, '99
>
> Ynd
>
> int-c MWG2033 Komatsuda, TAG 105:85, '02
>
> Zeo3
>
> glo-a
>
> rym1 MWG2134 Okada *et al*., Breeding Sci. 54:319, '04
>
> BIN4 BCD402B
>
> \*[Kap]{.mark} X83518 Müller *et al*., Nature 374:727, '95
>
> lbi2
>
> zeb2
>
> lgn3
>
> BIN5 BCD808B
>
> lgn4
>
> lks5
>
> eam9
>
> msg24
>
> BIN6 ABG484
>
> glf1
>
> [rym11]{.mark} MWG2134 Bauer *et al*., TAG 95:1263, '97
>
> [Mlg]{.mark} MWG032 Kurth *et al*., TAG 102:53, '01
>
> cer-zg
>
> brh2
>
> BIN7 bBE54A
>
> glf3
>
> frp
>
> min1
>
> blx4
>
> sid
>
> blx3
>
> BIN8 BCD453B
>
> blx1
>
> BIN9 ABG319A
>
> ert1
>
> BIN10 KFP221
>
> \*[mlo]{.mark} P93766 Bueschges *et al*., Cell 88:695, '97
>
> BIN11 ABG397
>
> BIN12 ABG319C
>
> [Hsh]{.mark} HVM067 Costa *et al*., TAG 103:415, '01
>
> Hln
>
> \*[sgh1]{.mark}(ZCCT-H; HvSnf2) Zitzewitz *et al*., PMB 59:449, '05
>
> yhd1
>
> BIN13 \*[Bmy1]{.mark} pcbC51 Kleinhofs *et al*., TAG 86:705, '93
>
> [rym8]{.mark} MWG2307 Bauer *et al*., TAG 95:1263, '97
>
> [rym9]{.mark} MWG517 Bauer *et al*., TAG 95:1263, '97
>
> Wsp3
>
> Chr. 5 (1H)
>
> BIN1 Tel5P
>
> Rph4
>
> Mlra
>
> Cer-yy
>
> [Sex76]{.mark} Hor2 Netsvetaev BGN 27:51, '97
>
> [Hor5]{.mark} Hor5 Kleinhofs *et al*., TAG 86:705, '93
>
> BIN2 MWG938
>
> \*[Hor2]{.mark} Hor2 Kleinhofs *et al*., TAG 86:705, '93
>
> [Rrs14]{.mark} Hor2 Garvin *et al*., Plant Breed. 119:193-196, '00
>
> \*[Mla6]{.mark} AJ302292 Halterman *et al*., Plt J. 25:335, '01
>
> BIN3 MWG837
>
> \*[Hor1]{.mark} Hor1 Kleinhofs *et al*., TAG 86:705, '93
>
> Rps4
>
> Mlk
>
> BIN4 ABA004
>
> Lys4
>
> BIN5 BCD098
>
> Mlnn; msg31; sls; msg4; fch3;
>
> BIN6 Ica1
>
> amo1
>
> BIN7 JS074
>
> clh
>
> vrs3
>
> Ror1 ABG452 Collins *et al*., Plt. Phys. 125:1236, '01
>
> \*[Sgh3]{.mark} (HvBM5B) Zitzewitz *et al*., PMB 59:449, '05
>
> BIN8 Pcr2
>
> fst2
>
> cer-zi
>
> cer-e
>
> ert-b
>
> MlGa
>
> msg1
>
> xnt7
>
> BIN9 Glb1
>
> \*[nec1]{.mark} BF630384 Rostoks *et al*., MGG 275:159, '06
>
> BIN10 DAK123B
>
> abo1
>
> Glb1
>
> BIN11 PSR330
>
> wst5
>
> cud2
>
> BIN12 MWG706A
>
> rlv
>
> lel1
>
> BIN13 BCD1930
>
> [Blp]{.mark} ABC261 Costa *et al*., TAG 103:415, '01
>
> BIN14 ABC261
>
> fch7
>
> trd
>
> eam8
>
> Chr. 6 (6H)
>
> BIN1 ABG062
>
> \*[Nar1]{.mark} X57845 Kleinhofs *et al*., TAG 86:705, '93
>
> abo15
>
> BIN2 ABG378B
>
> [nar8]{.mark} ABG378B Kleinhofs BGN 27:105, '96
>
> nec3
>
> Rrs13
>
> BIN3 MWG652A
>
> BIN4 DD1.1C
>
> msg36
>
> BIN5 ABG387B
>
> nec2
>
> ant21
>
> msg6
>
> eam7
>
> BIN6 Ldh1
>
> [rob]{.mark} HVM031 Costa *et al*., TAG 103:415, '01
>
> sex1
>
> gsh4
>
> ant13
>
> [cul2]{.mark} Crg4(KFP128) Babb & Muehlbauer BGN 31:28, '01
>
> fch11
>
> mtt5
>
> abo14
>
> BIN7 ABG474
>
> BIN8 ABC170B
>
> BIN9 \*[Nar7]{.mark} X60173 Warner *et al*., Genome 38:743, '95
>
> \*[Amy1]{.mark} JR115 Kleinhofs *et al*., TAG 86:705, '93
>
> \*[Nir]{.mark} pCIB808 Kleinhofs *et al*., TAG 86:705, '93
>
> mul2
>
> cur3
>
> BIN10 MWG934
>
> lax-b
>
> raw5
>
> cur1
>
> BIN11 Tef1
>
> BIN12 xnt5
>
> Aat2
>
> BIN13 [Rph11]{.mark} Acp3 Feuerstein *et al*., Plant breed. 104:318,
> '90
>
> lax-c
>
> BIN14 DAK213C
>
> dsp9
>
> Chr. 7 (5H)
>
> BIN1 DAK133
>
> abo12
>
> msg16
>
> ddt
>
> BIN2 MWG920.1A
>
> dex1
>
> msg19
>
> nld
>
> fch6
>
> glo-b
>
> BIN3 [cud1]{.mark} ABG705A
>
> lys3
>
> fst1
>
> blf1
>
> vrs2
>
> BIN4 ABG395
>
> cer-zj
>
> cer-zp
>
> msg18
>
> wst2
>
> [Rph2]{.mark} ITS1 Borovkova *et al*., Genome 40:236, '97
>
> [lax-a]{.mark} PSR118 Laurie *et al*., TAG 93:81, '96
>
> com1
>
> ari-e
>
> ert-g
>
> ert-n
>
> BIN5 Ltp1
>
> [rym3]{.mark} MWG028 Saeki *et al*., TAG 99:727, '99
>
> BIN6 WG530
>
> BIN7 ABC324
>
> BIN8 ABC302A
>
> BIN9 BCD926
>
> [srh]{.mark} ksuA1B Kleinhofs *et al*., TAG 86:705, '93
>
> cer-i
>
> mtt2
>
> lys1
>
> cer-t
>
> dsk
>
> var1
>
> cer-w
>
> Eam5
>
> BIN10 ABG473
>
> raw1
>
> msg7
>
> BIN11 MWG514B
>
> [Rph9/12]{.mark}ABG712 Borokova *et al*., Phytopath. 88:76, '98
>
> \*[Sgh2]{.mark} (HvBM5A) Zitzewitz *et al*., PMB 59:449, '05
>
> \*[Ror2]{.mark} AY246906 Collins *et al*., Nature 425:973, '03
>
> lbi1
>
> Rha4
>
> raw2
>
> BIN12 WG908
>
> none
>
> BIN13 ABG496
>
> [rpg4]{.mark} ARD5303 Druka *et al*., unpublished
>
> [RpgQ]{.mark} ARD5304 Druka *et al*., unpublished
>
> BIN14 ABG390
>
> var3
>
> BIN15 ABG463
>
> BIN markers are indicated
>
> \* - indicates the gene has been cloned

References:

Babb, S.L. and G.J. Muehlbauer. 2001. Map location of the Barley
Tillering Mutant *uniculm2* (*cul2*) on Chromosome 6H. BGN31:28.

Bauer, E., J. Weyen, A. Schiemann, A. Graner, and F. Ordon. 1997.
Molecular mapping of novel resistance genes against Barley Mild Mosaic
Virus (BaMMV). Theor. Appl. Genet. 95:1263-1269.

Borovkova, I.G., Y. Jin, B.J. Steffenson, A. Kilian, T.K. Blake, and A.
Kleinhofs. 1997. Identification and mapping of a leaf rust resistance
gene in barley line Q21861. Genome 40:236-241.

Borokova, I.G., Y. Jin, and B.J. Steffenson. 1998. Chromosomal Location
and Genetic Relationship of Leaf Rust Resistance Genes *Rph9* and
*Rph12* in Barley. Phytopathology 88:76-80.

Börner, A., V. Korzun, S. Malyshev, V. Ivandic, and A. Graner. 1999.
Molecular mapping of two dwarfing genes differing in their GA response
on chromosome 2H of barley. Theor. Appl. Genet. 99:670-675.

Brueggeman, R., N. Rostoks, D. Kudrna, A. Kilian, F. Han, J. Chen, A.
Druka, B. Steffenson, and A. Kleinhofs. 2002. The barley stem-rust
resistance gene *Rpg1* is a novel disease-resistance gene with homology
to receptor kinases. Proc. Natl. Acad. Sci. USA 99:9328-9333.

Brunner, S., B. Keller, and C. Feuillet. 2000. Molecular mapping of the
Rph7.g leaf rust resistance gene in barley (*Hordeum vulgare* L.).
Theor. Appl. Genet. 101:763-788.

Büschges, R., K. Hollricher, R. Panstruga, G. Simons, M. Wolter, A.
Frijters, R. van Daelen, T. van der Lee, P. Diergaarde, J. Groenendijk,
S. Töpsch, P. Vos, F. Salamini, and P. Schulze-Lefert. 1997. The barley
*mlo* gene: A novel control element of plant pathogen resistance. Cell
88:695-705.

Bulgarelli, D., N.C. Collins, G. Tacconi, E. Dellaglio, R. Brueggeman,
A. Kleinhofs, A.M. Stanca, and G. Vale. 2004. High resolution genetic
mapping of the leaf stripe resistance gene Rdg2a in barley. Theor. Appl.
Genet. 108:1401-1408.

Burton, R.A., S.M. Wilson, M. Hrmova, A.J. Harvey, N.J. Shirley, A.
Medhurst, B.A. Stone, E.J. Newbigin, A. Bacic, and G.B. Fincher. 2006.
Cellulose synthase-like CslF genes mediate the synthesis of cell wall
(1,3; 1,4)-b-D-glucans. Science 311:1940-1943.

Collins, N.C., N.G. Paltridge, C.M. Ford, and R.H. Symons.1996. The
*Yd2* gene for barley yellow dwarf virus resistance maps close to the
centromere on the long arm of barley chromosome 3. Theor. Appl. Genet.
92:858-864.

Collins, N.C., T. Lahaye, C. Peterhänsel, A. Freialdenhoven, M. Corbitt,
and P. Schulze-Lefert. 2001. Sequence haplotypes revealed by
sequence-tagged site fine mapping of the *Ror1* gene in the centromeric
region of barley chromosome 1H. Plant Physiology 125:1236-1247.

Collins, N.C., H. Thordal-Christensen, V. Lipka, S. Bau, E. Kombrink,
J-L. Qiu, R. Hückelhoven, N. Stein, A. Freialdenhoven, S.C. Somerville,
and P. Schulze-Lefert. 2003. Snare-protein-mediated disease resistance
at the plant cell wall. Nature 425:973-976.

Costa, J.M., A. Corey, M. Hayes, C. Jobet, A. Kleinhofs, A.
Kopisch-Obusch, S.F. Kramer, D. Kudrna, M. Li, O. Piera-Lizaragu, K.
Sato, P. Szues, T. Toojinda, M.I. Vales, and R.I. Wolfe. 2001. Molecular
mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic
doubled-haploid population. Theor. Appl. Genet. 103:415-424.

Drescher, A., V. Ivandic, U. Walther, and A. Graner. 2000.
High-resolution mapping of the Rph16 locus in barley. p. 95-97. *In*: S.
Logue (ed.) Barley Genetics VIII. Volume II. Proc. Eigth Int. Barley
Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide
University, Glen Osmond, South Australia.

Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005.
Characterization and molecular mapping of genes determining semidwarfism
in barley. J. Heredity 96:654-662.

Edwards, M.C. and B.J. Steffenson. 1996. Genetics and mapping of barley
stripe mosaic virus resistance in barley. Phytopath. 86;184-187.

Feuerstein, U., A.H.D. Brown, and J.J. Burdon. 1990. Linkage of rust
resistance genes from wild barley (*Hordeum spontaneum*) with isoenzyme
markers. Plant. Breed. 104:318-324.

Freialdenhoven, A., B. Scherag, K. Hollrichter, D.B. Collinge, H.
Thordal-Christensen, and P. Schulze-Lefert. 1994. *Nar-1* and *Nar-2*,
two loci required for *Mla12*-specified race-specific resistance to
powdery mildew in barley. Plant Cell 6:983-994.

Garvin, D.F., A.H.D. Brown, H. Raman, and B.J. Read. 2000. Genetic
mapping of the barley *Rrs14* scald resistance gene with RLFP, isozyme
and seed storage protein marker. Plant Breeding 119:193-196.

Genger, R.K., K. Nesbitt, A.H.D. Brown, D.C. Abbott, and J.J. Burdon.
2005. A novel scald resistance gene: genetic mapping of the Rrs15 scald
resistance derived from wild barley, Hordeum vulgare ssp. spontaneum.
Plant Breeding 124:137-141.

Giese, H., A.G. Holm-Jensen, H.P. Jensen, and J.Jensen. 1993.
Localisation of the Laevigatum powdery mildew resistance gene to barley
chromosome 2 by the use of RLFP markers. Theor. Appl. Genet. 85:897-900.

Graner, A. and A. Tekauz. 1996. RFLP mapping in barley of a dominant
gene conferring to scald (*Rynchosporium secalis*). Theor. Appl. Genet.
93:421-425.

Haltermann, D., F. Zhou, F. Wei, R.P. Wise, and P. Schulze-Lefert. 2001.
The MLA6 coiled coil, NBS-LRR protein confers *AvrMla6*-dependent
resistance specificity to *Blumeria graminis* f. sp. *hordei* in barley
and wheat. Plt. J. 25:335-348.

Hansson, A., C.G. Kannangara, D. von Wettstein, and M. Hansson. 1999.
Molecular basis for semidomiance of missense mutations in the XANTHA-H
(42-kDa) subunit of magnesium chelatase. Proc. Natl. Acad. Sci. USA
96:1744-1749.

Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells,
and S.D. Tanksley. 1991. Construction of a restriction fragment length
polymorphism map for barley (*Hordeum vulgare*). Genome 34:437-447.

Ivandic, V., S. Malyshev, V. Korzun, A. Graner, and A. Börner. 1999.
Comparative mapping of a gibberellic acid-insensitive dwarfing gene
(*Dwf2*) on chromosome 4HS in barley. Theor. Appl. Genet. 98:728-731.

Kanyuka, K., A. Druka, D..G. Caldwell, A. Tymon, N. McCallum, R. Waugh,
and M. J. Adams. 2005. Evidence that the recessive bymovirus resistance
locus *rym4* in barley corresponds to the eukaryotic translation
initiation factor 4E gene. Molecular Plant pathology 6:449-458.

Kleinhofs, A., A. Kilian, M.A. Saghai Marrof, R.M. Biyashev, P. Hayes,
F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev,
L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorells, M.
Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen, and B.J. Steffenson.
1993. A molecular, isozyme and morphologicsl map of the barley (*Hordeum
vulgare*) genome. Theor. Appl. Genet. 86:705-712.

Kleinhofs, A. 1996. Integrating Barley RFLP and Classical Marker Maps.
Coordinator's report. BGN27:105-112.

Kleinhofs, A. 2002. Integrating Molecular and
Morphological/Physiological Marker Maps. Coordinator's Report.
BGN32:152-159.

Komatsuda, T., W. Li, F. Takaiwa, and S. Oka. 1999. High resolution map
around the *vrs1* locus controlling two- and six-rowed spike in barley.
(*Hordeum vulgare*). Genome 42:248-253.

Komatsuda, T., and Y. Mano. 2002. Molecular mapping of the intermedium
spike-c (*int-c*) and non-brittle rachis 1 (*btr1*) loci in barley
(*Hordeum vulgare* L.). Theor. Appl Genet. 105:85-90.

Komatsuda, T., M. Pourkheirandish, C. He, P. Azhaguvel, H. Kanamori, D.
Perovic, N. Stein, A. Graner, U. Lundqvist, T. Fujimura, M. Matsuoka, T.
Matsumoto, and M. Yano. 2006. Map-based cloning of the barley six-rowed
spike gene *vrs1*. Plant and Animal Genome XIV. San Diego, USA, Abstract
W13, p.10.

Kretschmer, J.M., K.J. Chalmers, S. Manning, A. Karakousis, A.R. Barr,
A.K.M.R. Islam, S.J. Logue, Y.W. Choe, S.J. Barker, R.C.M. Lance, and P.
Langridge. 1997. RFLP mapping of the *Ha2* cereal cyst nematode
resistance in barley.Theor. Appl. Genet. 94:1060-1064.

Kurth, J., R. Kolsch, V. Simons, and P. Schulze-Lefert. 2001. A
high-resolution genetic map and a diagnostic RFLP marker for the *Mlg*
resistance locus to powdery mildew in barley. Theor. Appl. Genet.
102:53-60.

Larson, S.R., K.A. Young, A. Cook, T.K. Blake, and V. Raboy. 1998.
Linkage mapping of two mutations that reduce phytic acid content of
barley grain. Theor. Appl. Genet. 97:141-146.

Laurie, D.A., N. Pratchett, C. Romero, E. Simpson, and J.W. Snape. 1993.
Assignment of the *denso* dwarfing gene to the long arm of chromosome 3
(3H) of barley by use of RFLP markers. Plant. Breed. 111:198-203.

Laurie, D.A., N. Pratchett, J.H. Bezan, and J.W. Snape. 1994. Genetic
analysis of a photoperiod response gene on the short arm of chromosome 2
(2H) of Hordeum vulgare (barley). Heredity 72:619-627.

Laurie, D.A., N. Pratchett, R.A. Allen, and S.S. Hantke. 1996. RFLP
mapping of the barley homeotic mutant *lax-a*. Theor. Appl. Genet.
93:81-85.

Li. M., D. Kudrna, and A. Kleinhofs. 2000. Fine mapping of a Semi-dwarf
gene Brachytic1 in barley. p. 72-74. *In*: S. Logue (ed.) Barley
Genetics VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp.
Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen
Osmond, South Australia.

Mammadov, J.A., B. J. Steffenson, and M.A. Saghai Maroof. 2005. High
resolution mapping of the barley leaf rust resistance gene Rph5 using
barley expressed sequence tags (ESTs) and synteny with rice. Theor.
Appl. Genet. 111:1651-1660.

Murakami, S., K. Matsui, T. Komatsuda, and Y. Furuta. 2005. AFLP-based
STS markers closely linked to a fertility restoration locus (Rfm1) for
cytoplasmic male sterility in barley. Plant Breeding 124:133-136.

Müller, K.J., N. Romano, O. Gerstner, F. Gracia-Maroto, C. Pozzi, F.
Salamini, and W. Rhode. 1995. The barley *Hooded* mutation caused by a
duplication in a homeobox gene intron. Nature 374:727-730.

Netsvetaev, V.P. 1997. High lysine mutant of winter barley - L76.
BGN27:51-54.

Okada, Y., R. Kanatani, S. Arai, and I Kazutoshi. 2004. Interaction
between barley mosaic disease-resistance genes *rym1* and *rym5*, in the
response to BaYMV strains. Breeding Science 54 (4):319-324.

Park, R.F. and A. Karakousis. 2002. Characterization and mapping *Rph19*
conferring resistance to *Puccinia hordei* in the cultivar 'Rea1' and
several Australian barley. Plant Breeding 121:232-236.

Pratchett, N. and D.A. Laurie. 1994. Genetic map location of the barley
developmental mutant *liguleless* in relation to RFLP markers. Hereditas
120:35-39.

Rostoks, N., D. Schmierer, S. Mudie, T. Drader, R. Brueggeman, D.
Caldwell, R. Waugh, and A. Kleinhofs. 2006. Barley necrotic locus *nec1*
encodes the cyclic nucleotide-gated ion channel 4 homologous to the
*Arabidopsis Hlm1.* Mol.Gen. Genomics 275:159-168.

Saeki, K., C. Miyazaki, N. Hirota, A. Saito, K. Ito, and T. Konishi.
1999. RFLP mapping of BaYMV resistance gene *rym3* in barley (*Hordeum
vulgare*). Theor. Appl. Genet. 99:727-732.

Saisho, D., K.-I. Tanno, M. Chono, I. Honda, H. Kitano, and K. Takeda.
2004. Spontaneous Brassinolide-insensitive barley mutants 'uzu' adapted
to East Asia. Breeding Science 54(4): 409-416.

Schmierer, D., A. Druka, D. Kudrna, and A. Kleinhofs. 2001. Fine Mapping
of the *fch12* chlorina seedling mutant. BGN31:12-13.

Schweizer, G.F., M. Baumer, G. Daniel, H. Rugel, and M.S. Röder. 1995.
RFLP markers linked to scald (*Rhynchosporium secalis*) resistance gene
*Rh2* in barley. Theor. Appl. Genet. 90:920-922.

Smilde, W.D., A. Tekauz, and A. Graner. 2000. Development of a high
resolution map for the *Rh* and *Pt* resistance on barley Chromosome 3H.
p. 178-180. *In*: S. Logue (ed.) Barley Genetics VIII. Volume II. Proc.
Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite
Campus, Adelaide University, Glen Osmond, South Australia.

Soule, J.D., D.A. Kudrna, and A. Kleinhofs. 2000. Isolation, mapping,
and characterization of two barley multiovary mutants. J. Heredity
91:483-487.

Senthil, N. and T. Komatsuda. 2005. Inter-subspecific maps of
non-brittle rachis gene btr1/btr2 using occidental, oriental and wild
barley lines. Euphytica 145:215-220.

Stein, N., D. Perovic, J. Kumlehn, B. Pellio, S. Stracke, S. Streng, F.
Ordon, and A. Graner. 2005. The eukaryotic translation initian factor 4E
confers multiallelic recessive Bymovirus resistance in *Hordeum vulgare*
(L.). The Plant Journal 42:912-922.

Tohno-oka, T., M. Ishit, R. Kanatani, H. Takahashi, and K. Takeda. 2000.
Genetic Analysis of photoperiotic response of barley in different
daylength conditions. p.239-241. *In*: S. Logue (ed.) Barley Genetics
VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp. Adelaide. Dept.
Plant Science, Waite Campus, Adelaide University, Glen Osmond, South
Australia.

Toojinda, T., L.H. Broers, X.M. Chen, P.M. Hayes, A. Kleinhofs, J.
Korte, D. Kudrna, H. Leung, R.F. Line, W. Powell, L. Ramsey, H. Vivar,
and R. Waugh. 2000. Mapping quantitative and qualitative disease
resistance genes in a doubled haploid population of barley (*Hordeum
vulgare*). Theor. Appl. Genet. 101:580-589.

Warner, R.L., D.A. Kudrna, and A. Kleinhofs. 1995. Association of the
NAD(P)H-bispecific nitrate reductase structural gene with the *Nar7*
locus in barley. Genome 38:743-746.

Weerasena, J.S., B.J. Steffenson, and A.B. Falk. 2004. Conversion of an
amplified fragment length polymorphism marker into a c-dominant marker
in mapping *Rph15* gene conferring resistance to barley leaf rust,
*Puccinia hordei* Otth. Theor. Appl. Genet. 108:712-719.

Williams, K.J., A. Lichon, P. Gianquitto, J.M. Kretschmer, A.
Karakousis, S. Manning, P. Langridge, and H. Wallwork. 1999.
Identification and mapping of a gene conferring resistance to the spot
form of net blotch (*Pyrenophora teres* f. *maculata*) in barley. Theor.
Appl. Genet. 99: 323-327.

Williams, K., P. Bogacki, L. Scott, A. Karakousis, and H. Wallwork.
2001. Mapping of a gene for leaf scald resistance in barley line
'B87/14' and validation of microsatellite and RFLP markers for
marker-assisted selection. Plant Breed. 120:301-304.

Zitzewitz, J. von, P. Scucs, J. Dobcov, L. Yan, E. Francia, N.
Pecchioni, A. Casas, T.H.H. Chen, P.M. Hayes, and J.S. Skinner. 2005.
Molecular and structural characterization of barley vernalization genes.
Plant Molecular Biology 59:449-467.

Zhong, S.B., R.J. Effertz, Y. Jin, J.D. Franckowiak, and B.J.
Steffenson. 2003. Molecular mapping of the leaf rust resistance gene
*Rph6* in barley and its linkage relationships with *Rph5* and *Rph7*.
Phytopathology 93 (5):604-609.

**Coordinator's report: Barley Genetic Stock Collection**

A. Hang and K. Satterfield

USDA-ARS, National Small Grains Germplasm Research Facility,

Aberdeen, Idaho 83210, USA

e-mail: anhang@uidaho.edu

In 2005, 373 barley genetic stocks were planted in the field and in the
greenhouse for evaluation and for seed increase.

Four mapping populations including SSD F6 OSU 1/Harr, SSD F6 OSU 2/Harr,
SSD F6 OSU 11/Harr and SSD F6 OSU 15/Harr derived from crosses between
*Hordeum vulgare subps. Spontaneum* with the cultivar "Harrington" and
142 *H. spontaneum* introgression lines BC2 S1 and BC2 S5 were obtained
from Dr. Pat Hayes, OSU and maintained at Aberdeen. Two populations, SSD
F6 OSU 1/Harr and SSD F6 2/Harr were planted in the field for seed
increase.

One hundred thirty-two samples of barley genetic stocks were shipped to
researchers in 2005.

**Coordinator's report: Trisomic and aneuploid stocks**

A. Hang

USDA-ARS, National Small Grains Germplasm Research Facility

Aberdeen, Idaho 83210, USA

e-mail: anhang@uidaho.edu

There is no new information about trisomic and aneuploid stocks. A list
of these stocks are available in BGN 25:104. Seed request for this stock
should be sent to the coordinator.

**Coordinator's report: Autotetraploids**

Wolfgang Friedt, Institute of Crop Science and Plant Breeding I.

Justus-Liebig-University, Heinrich-Buff-Ring 26-32

DE-35392 Giessen, Germany

e-mail: <wolfgang.friedt@agrar.uni-giessen.de>

Fax: +49(0)641-9937429

The collection of barley autotetraploids (exclusively spring types)
described in former issues of BGN is maintained at the Giessen Field
Experiment Station of our institute. The set of stocks, i.e.
autotetraploids (4n) and corresponding diploid (2n) progenitors (if
available) have last been grown in the field for seed multiplication in
summer 2000. Limited seed samples of the stocks are available for
distribution.

**Coordinator's report: The Genetic Male Sterile Barley Collection**

M.C. Therrien

Agriculture and Agri-Food Canada

Brandon Research Centre

Box 1000A, RR#3, Brandon, MB

Canada R7A 5Y3

E-mail: [MTherrien@agr.gc.ca]{.underline}

The GMSBC has been at Brandon since 1992. If there are any new sources
of male-sterile genes that you are aware of, please advice me, as this
would be a good time to add any new source to the collection. For a list
of the entries in the collection, simply [E-mail]{.underline} me at the
above adress. I can send the file (14Mb) in Excel format. We continue to
store the collection at -20^o^C and will have small (5 g) samples
available for the asking. Since I have not received any reports or
requests the last years, there is absolutely no summary in my report.

**Coordinator's report: Translocations and balanced tertiary trisomics**

Andreas Houben

# Institute of Plant Genetics and Crop Plant Research

06466 Gatersleben, Germany

email: <houben@ipk-gatersleben.de>

Chromosome 5H of *Hordeum vulgare* carries a gene(s) that accelerates
heading in a wheat background. To introduce the early heading gene(s) of
barley into the wheat genome, the Japanese scientists S. Taketa and
colleagues attempted to induce homoeologous recombination between wheat
and 5H chromosomes by 5B nullisomy. A nullisomic 5B, trisomic 5A,
monosomic 5H plant (2n = 42) was produced from systematic crosses
between aneuploid stocks of wheat group 5 chromosomes. Twelve plants
(1.8%) were selected as putative wheat-barley 5H recombinants.
Cytological analyses using fluorescence in situ hybridization and
C-banding revealed that 6 of the progeny lines are true homoeologous
recombinants between the long arms of chromosomes 5D and 5H. The 6
cytologically confirmed recombinant lines included only 2 types (3 lines
each), which were reciprocal products derived from exchanges at the same
distal interval defined by two flanking markers. One type had a small
5HL segment translocated to the 5DL terminal, and the other type had a
small terminal 5DL segment translocated to the 5HL terminal. In the
latter type, the physical length of translocated barley segments
slightly differed among lines.

There were no requests for samples of balanced tertiary trisomics or
tranlocation lines. The collection is being maintained in cold storage.
To the best knowledge of the coordinator, there are no new publications
dealing with balanced tertiary trisomics in barley. Limited seed samples
are available any time, and requests can be made to the coordinator.

**Reference:**

[Taketa,
S](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Taketa+S&curr_doc=11/1&Form=FullRecordPage&doc=11/1),
T.
[Awayama](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Awayama+T&curr_doc=11/1&Form=FullRecordPage&doc=11/1)
, M.
[Ichii](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Ichii+M&curr_doc=11/1&Form=FullRecordPage&doc=11/1),
M.
[Sunakawa](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Sunakawa+M&curr_doc=11/1&Form=FullRecordPage&doc=11/1),
T.
[Kawahara](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Kawahara+T&curr_doc=11/1&Form=FullRecordPage&doc=11/1),
and K.
[Murai](http://wos15.isiknowledge.com/CIW.cgi?SID=Y2C6cEMa7mlk@c63AC8&Func=OneClickSearch&field=AU&val=Murai+K&curr_doc=11/1&Form=FullRecordPage&doc=11/1).
2005. Molecular cytogenetic identification of nullisomy 5B induced
homoeologous recombination between wheat chromosome 5D and barley
chromosome 5H. Genome 48: 115-124.

**Coordinator's report: *Eceriferum* Genes**

Udda Lundqvist

### SvalöfWeibul AB

### SE-268 81 Svalöv, Sweden

e-mail: <udda@ngb.se> or [udda@nordgen.org]{.underline}

No research work on gene localization has been reported on the
collections of *Eceriferum* and *Glossy* genes since the latest reports
in Barley Genetics Newsletter (BGN). All information and descriptions
done in Barley Genetics Newsletter (BGN) Volume 26 are valid and still
up-to-date. The database of the Swedish collection has been updated
during the last months and will soon be searchable within International
European databases. All Swedish *Eceriferum* alleles can be seen in the
SESTO database of the Nordic Gene Bank. As my possibilities in searching
literature are very limited, I apologize if I am missing any important
papers. Please send me notes of publications and reports to include in
next year's reports. Descriptions, images and graphic chromosome map
displays of the *Eceriferum* and *Glossy* genes are available in the
AceDB database for Barley Genes and Barley Genetic Stocks, and they get
currently updated. Its address is found by:
[www.untamo.net/bgs]{.underline}

Every research of interest in the field of *Eceriferum* genes, 'Glossy
sheath' and 'Glossy leaf' genes can be reported to the coordinator as
well. Seed requests regarding the Swedish mutants can be forwarded to
the coordinator <udda@ngb.se> or [udda@nordgen.org]{.underline} or to
the Nordic Gene Bank, [www.nordgen.org/ngb](mailto:nordgen@ngb.se), all
others to the Small Grain Germplasm Research Facility (USDA-ARS),
Aberdeen, ID 83210, USA, <anhang@uida.edu> or to the coordinator at any
time.

**Coordinator's report: Nuclear genes affecting the chloroplast**

Mats Hansson

Department of Biochemistry,

Lund University, Box 124,

SE-22100 Lund, Sweden

E-mail: mats.hansson@biokem.lu.se

Chlorophyll biosynthesis is a process involving approximately 20
different enzymatic steps. One of the least understood enzymatic steps
is formation of the isocyclic ring, which is a characteristic feature of
all chlorophyll molecules. In chloroplasts this is an aerobic reaction
catalyzed by Mg-protoporphyrin IX monomethyl ester cyclase. Barley
mutants were employed to study this enzyme (Rzeznicka et al. 2005). An
*in vitro* assay for the aerobic cyclase reaction required both
membrane-bound and soluble components from the chloroplasts. Extracts
from barley mutants at the *Xantha-l* and *Viridis-k* loci showed no
cyclase activity. Fractionation of isolated plastids by Percoll gradient
centrifugation showed that both *xantha-l* and *viridis-k* mutants are
defective in components associated with chloroplast membranes. The
evidence suggests that the aerobic cyclase requires at least one soluble
and two membrane-bound components. The *Xantha-l* gene was located to
the short arm of barley chromosome 3H. The gene was further cloned and
sequenced and the mutations *xantha-l.*35, *-l.81* and *--l.*82 were
characterized at the DNA level. The study connected for the first time
biochemical and genetic data as it demonstrated that *Xantha-l* encodes
a membrane-bound cyclase subunit.

The stock list and genetic information presented in the Barley Genetics
Newsletter 21: 102-108 is valid and up-to-date. Requests for stocks
available for distribution are to be either sent to:

Dr. Mats Hansson

Department of Biochemistry

Lund University

Box 124

SE-22100 Lund, SWEDEN

Phone: +46-46-222 0105

Fax: +46-46-222 4534

E-mail: <Mats.Hansson@biokem.lu.se>

or to

Nordic Gene Bank

Box 41

SE-23053 Alnarp

Sweden

Phone: +46-40-536640

FAX: +46-40-536650

[www.nordgen.org/ngb]{.underline}

**Reference:**

Rzeznicka, K., C. J. Walker, T. Westergren, G. C. Kannangara, D. von
Wettstein, S. Merchant, S. P. Gough, and M. Hansson. 2005. *Xantha-l*
encodes a membrane protein subunit of the aerobic Mg-protoporphyrin IX
monomethyl ester cyclase in the chlorophyll biosynthetic pathway. Proc.
Natl. Acad. Sci. USA 102:5886-5891.

### Coordinator's report: Ear morphology genes

Udda Lundqvist

SvalöfWeibull AB

# SE-268 81 Svalöv, Sweden

e-mail: <udda@ngb.se> [or udda@nordgen.org]{.underline}

No new research on gene localization or descriptions on different
morphological genes have been reported since the latest reports in
Barley Genetics Newsletter (BGN) or in the AceDB database for Barley
Genes and Genetic Stocks.. All descriptions made in the BGN volumes 26,
28, 29, 32 and 35 are still up-to-date and valid. The databases of the
Swedish Ear morphology genes are currently updated and will be
searchable within International European databases in the future. All
different types and characters with its many alleles of the Swedish ear
morphology genes are found in the SESTO database of the Nordic Gene
Bank. Also, a survey list of the different Swedish ear morphology genes
are published in the last volume of Barley Genetics Newsletter, BGN
35:150-154. As my possibilities in searching literature are very
limited, I apologize if I am missing any important reports or papers. I
would like to call on the barley community to assist me by sending notes
of publications and reports to include in next year's reports.
Descriptions, images and graphic chromosome map displays of the Ear
morphology genes are also available in the AceDB database for Barley
Genes and Barley Genetic Stocks. They get currently updated and are
searchable under the address: [www.untamo.net/bgs]{.underline}

Every research of interest in the field of Ear morphology genes can be
reported to the coordinator as well. Seed requests regarding the Swedish
mutants can be forwarded to the coordinator <udda@ngb.se> or
[udda@nordgen.org]{.underline} or to the Nordic Gene Bank,
[www.nordgen.org/ngb]{.underline}. all others to the Small Grain
Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
[anhang@uida.edu](mailto:abhang@uida.edu) or to the coordinator at any
time.

**Coordinator's report: Semidwarf genes**

** **

**J**.D. Franckowiak

Department of Plant Sciences

North Dakota State University

Fargo, ND 58105, USA.

e-mail: j.franckowiak@ndsu.nodak.edu

Dahleen *et al*. (2005) studied 27 mutants from various sources that
were placed in the brachytic (*brh*) group of semidwarf mutants. The
mutants were backcrossed into 'Bowman' prior to this study to facilitate
allelism studies and their phenotypic characterization. The traits
studied included plant height; awn, peduncle, and rachis internode
length; leaf width and length; lodging; kernels per spike; grain yield;
and kernel weight. Based on allelism tests and molecular mapping studies
using simple sequence repeat (SSR) markers, the mutants occurred at 18
different loci. Eight of the loci had been identified in previous
studies and ten were new loci. Using small F2 populations, SSR markers
were mapped within 30 cM of all loci except the *brh15.u* mutant. The
brachytic mutants were located as follows: *ert-t* (*brh3.y*), *brh4.j*,
and *brh10.l* on chromosome 2H; *brh8.ad* and *brh14.q* on 3H; *brh2*
(*ari-l.3*), *brh5.m*, and *brh9.k* on 4H; *brh6.r*, *brh7.w*,
*brh11.o*, *brh12.p*, *brh17.ab*, and *brh18.ac* on 5H; and *brh1.z* and
*brh16.v* on 7H. The positional information suggested that one or two
clusters of brachytic loci may exist on 5H. Three of five loci that were
positioned earlier by linkage drag (Franckowiak, 1995) were found in a
similar position base on the SSR mapping data.

All of the *brh* mutants as evaluated in Bowman backcross-derived lines
were shorter than Bowman with an average height of 64.8 cm vs. 87.9 for
Bowman (Dahleen *et al*. 2005). All of the *brh* lines had shorter awns
and most had shorter peduncles and smaller kernels. Some of the *brh*
lines had shorter rachis internodes and short leaf blades. The majority
of the *brh* lines, 16 of 27, had lower grain yields than Bowman.
Although none of the *brh* lines was superior to Bowman, the *brh4*,
*brh6*, and *brh8* mutants seemed to be the most promising ones for
further agronomic evaluation.

Horsley *et al*. (2006) reported that the main plant height QTLs in a
'Foster'/CIho 4196 mapping population were near the *vrs1* locus on 2HL.
Dahleen *et al*. (2003) reported a plant height QTL in the same region
of 2H from a study of two- by six-rowed cross, ND9712//Foster/Zhedar 2.
The association between plant height and the six-rowed phenotype was
first reported as a linkage by Miyake and Imai in 1922 and has been
reported often since then (Franckowiak 1997). The locus symbol *hcm1* is
currently recommended. Horsley *et al*. (2006) provided some evidence
that more than one factor for reduced plant height is associated with
the *vrs1* locus in the Foster/CIho 4196 cross. They reported also that
they did not recover any short plants with a two-rowed spike type from a
large F2 population. Thus, it is still not clear whether the *hcm1*
locus exists or the six-rowed allele (*vsr1.a*) at *vrs1* locus has a
pleiotropic effect on plant height in warm environments.

Honda *et al*. (2003) found that treatment of barley near-isogenic lines
with the brassinosteroid (BR) growth regular caused leaf blade rolling
in normal barley and most barley semidwarf mutants. However, detached
leaf blade segments from dark grown plants with the *uzu1* gene did not
unroll after treatment in the leaf unroll test. In a subsequent study,
Chono *et al*. (2003) demonstrated that the response of *uzu1* mutants
to BR was caused by a base pair substitution in the *Hordeum vulgare*
BR-insensitive 1 (*HvBR11*) gene and an amino acid change in a highly
conserved residue in the kinase domain of the BR-receptor protein. The
*uzu1* lines have a missense mutation in the *HvBR11* gene.

Gottwald *et al*. (2004) reported that a gibberellic-acid insensitive
dwarf mutant, first described by Favret *et al*. (1976), is closely
linked to RFLP marker MWG2287 on 2HS near the centromere. The proposed
locus symbol for the GA insensitive mutant is *sdw3*, which replaced the
symbols *gai* and *GA-ins* used in earlier publications. The suggested
allele symbol is *sdw3.az* for the Hv287 line derived from the M.C. 90
mutant induced in M.C. 20. This region of 2HS is orthologous with a
highly conserved region on rice chromosome 7L. ESTs in this region were
used to identify three putative GA-related ORFs in rice that might
correspond to the *sdw3* locus. (Gottwald *et al*. 2004).

References:

Chono, M., I. Honda, H. Zeniya, K. Yoneyama, D. Saisho, K. Takeda, S.
Takatsuto, T. Hoshino, and Y. Watanabe. 2003. A semidwarf phenotype of
barley *uzu* results from a nucleotide substitution in the gene encoding
a putative bassinosteroid receptor. Plant Physiol. 133:1209-1219.

Dahleen, L.S., H.A. Agrama, R.D. Horsley, B.J. Steffenson, P.B. Schwarz,
A. Mesfin, and J.D. Franckowiak. 2003. Identification of QTLs associated
with Fusarium head blight resistance in Zhedar 2. Theor. Appl. Genet.
108:95-104.

Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005.
Characterization and molecular mapping of genes determining semidwarfism
in barley. J. Hered. 96:654-662.

Favret, E.A., G.C. Favret, and E.M. Malvarez. 1976. Genetic regulatory
mechanisms for seedling growth in barley. p. 37-41. p. 181-189. *In* H.
Gaul (ed.). Barley Genetics III. Proc. Third Int. Barley Genet. Symp.,
Garching, 1975. Verlag Karl Thiemig, München.

Franckowiak, J.D. 1995. Notes on linkage drag in Bowman backcross
derived lines of spring barley. BGN 24:63-70.

Franckowiak, J.D. 1997. BGS 77, short culm, *hcm*, revised. BGN 26:115.

Gottwald, S., N. Stein, A. Börner, T. Sasaki, and A. Graner. 2004. The
gibberellic-acid insensitive dwarfing gene *sdw3* of barley is located
on chromosome 2HS in a region that shows high colinearity with rice
chromosome 7L. Mol. Gen. Genomics 271:426-436.

Honda, I., H. Zeniya, K. Yoneyama, M. Chono, S. Kaneko, and Y. Watanaba.
2003. *Uzu* mutation in barley (*Hordeum vulgare* L.) reduces leaf
unrolling response to brassinole. Biosci. Biotechnol. Biochem.
67:1194-1197.

Horsley, R.D., D. Schmierer, C. Maier, D. Kudrna, C.A. Urrea, B.J.
Steffenson, P.B. Schwarz, J.D. Franckowiak, M.J. Green, B. Zhang, and A.
Kleinhofs. 2006. Identification of QTLs associated with Fusarium head
blight resistance in barley accession CIho 4196. Crop Sci. 46:145-156.

Coordinator's report: Early maturity genes

Udda Lundqvist

SvalöfWeibull AB

SE-268 81 Svalöv, Sweden

e-mail: <udda@ngb.se> or udda@nordgen.org

No new research on gene localization has been reported on the Early
maturity or Praematurum genes since the latest reports in Barley Genetic
Newsletter (BGN) or in the AceDB database for Barley Genes and Barley
Genetic Stocks. All information and descriptions made in the Barley
Genetics Newsletter are valid and up-to-date. As my possibilities in
searching literature are very limited, I apologize if I am missing any
important papers and reports. I would like to call on the barley
community to assist me by sending notes of publications and reports to
include in next year's report. Descriptions, images and graphic
chromosome map displays of the Early maturity or Praematurum genes are
available in the AceDB database for Barley Genes and Barley Genetic
Stocks. They get currently updated and are searchable under the address:
[www.untamo.net/bgs]{.underline}

Every research of interest in the field of Early maturity genes can be
reported to the coordinator as well. Seed requests regarding the Swedish
mutants can be forwarded to the coordinator or directly to the Nordic
Gene Bank, [www.nordgen.org/ngb]{.underline}, all others to the Small
Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
<anhang@uidaho.edu> or to the coordinator at any time.

**Coordinator's report : Wheat-barley genetic stocks**

A.K.M.R. Islam

Faculty of Agriculture, Food & Wine,

The University of Adelaide, Waite Campus,

Glen Osmond, SA 5064, Australia

e-mail: rislam@waite.adelaide.edu.au

The production of five different monosomic addition lines of *Hordeum
marinum* chromosomes to Chinese Spring wheat has been reported earlier.
It has now been possible to isolate five disomic addition lines (1Hm,
2Hm, 4Hm, 5Hm and 7Hm) from them and work is in progress to isolate the
two remaining (3Hm and 6Hm) addition lines. Apart from the production of
*H. marinum* x CS wheat amphiploid, it has also been possible to produce
amphiploid with commercial wheats, both common and durum (Islam and
Colmer, unpublished).