Addendum 1

Supplementary information on differences in gene expression between
Pr111 and Pr117, Pr14, Pr14C1, and Pr14C2 cell lines. : p \< 0.01; ^\*^:
p \< 0.001; n.s.: non significant differences

Name Pr111 vs Pr-117 Pr111 vs Pr-14 Pr111 vs Pr-14C2 Pr111 vs Pr-1C1

Ratio Ratio Ratio Ratio

Activating transc. Factor 1 1.6 n.s. 1.5 n.s. 1.3, n.s 2.10^\*^

Actopaxin 0.47^\*^ 0.43^\*^ 0.37^\*^ 0.47^\*^

Adducin-3 0.35 0.31^\*^ 0.34^\*^ 0.44^\*^

Adhesion integrin B7 0.46^\*^ 0.37^\*^ 0.41^\*^ 0.41^\*^

Adhesion lymphocyte 6 2.12^\*^ 3.30^\*^ 6.82^\*^ 9.21^\*^

Alpha actinin-4 0.40 0.40^\*^ 0.38^\*^ 0.70, n.s.

Alpha disintegrin and MP-9 0.42^\*^ 0.42^\*^ 0.45^\*^ 0.42^\*^

Annexin-2 0.45^\*^ 0.35^\*^ 0.42^\*^ 0.47

Annexin-4 0.29^\*^ 0.50 0.44^\*^ 0.7, n.s.

Annexin-5 0.48 0.42^\*^ 0.33^\*^ 0.8, n.s.

Annexin-6 0.46^\*^ 0.34^\*^ 0.29^\*^ 0.28^\*^

Annexin-7 0.45^\*^ 0.36^\*^ 0.50^\*^ 0.32^\*^

Apoptosis NIP-3 0.43 0.18^\*^ 0.24^\*^ 0.25^\*^

Avian leukemia oncogene 1.02, n.s. 0.90, n.s. 1.33, n.s. 2.77^\*^

AXL receptor tyrosine kinase 1.2, n.s. 0.09^\*^ 0.09^\*^ 0.08^\*^

Beta-catenin 0.40 0.52 0.51 0.53

Bcl-2/adenov 19kDa (NIP-2) 0.49 0.32^\*^ 0.43^\*^ 0.34^\*^

Bcl-associated death promoter 0.46^\*^ 0.9, n.s. 0.70, n.s. 0.85, n.s.

Bcl-2-related ovarian killer 2.00 2.21^\*^ 1.20, n.s. 2.20^\*^

c-fos 1.40, n.s. 1.40, n.s. 1.10, n.s. 2.25^\*^

c-myc 2.20 2.23^\*^ 2.5^\*^ 3.5^\*^

Cadherin-E 0.17^\*^ 0.50 0.48 0.52

Cadherin-N 0.38^\*^ 0.29^\*^ 0.42^\*^ 0.31^\*^

Cadherin-P 2.04 2.18^\*^ 2.30^\*^ 3.4^\*^

Calcyclin 0.23^\*^ 0.16^\*^ 0.18^\*^ 0.32^\*^

Calponin-2 0.40 0.43^\*^ 0.28^\*^ 0.47^\*^

cAMP inducible gene-1 1.43 2.50^\*^ 2.74^\*^ 3.51^\*^

Carbonic anhydrase-6 5.49^\*^ 1.23, n.s. 0.88, n.s. 1.40, n.s.

Caspase 11 3.96^\*^ 0.47^\*^ 0.25^\*^ 0.40^\*^

Cathepsin-C 1.20, n.s. 0.1^\*^ 0.21^\*^ 0.4

Cathepsin-L 0.44^\*^ 0.41^\*^ 0.26^\*^ 0.9, n.s.

Cathepsin-M 0.44 0.37^\*^ 0.39 0.5^\*^

Cathepsin Z 0.60, n.s. 0.47^\*^ 0.52 0.44^\*^

CEA-cell adhesion molec.-1 1.4, n.s. 1.8, n.s. 2.1 2.3^\*^

Claudin-6 1.30, n.s. 0.6, n.s. 1.50, n.s. 4.15^\*^

Cyclin D 0.12^\*^ 0.14^\*^ 0.12^\*^ 0.16^\*^

Cyclin G 0.17^\*^ 0.17^\*^ 0.18^\*^ 0.35^\*^

Cyclin H 1.2, n.s. 1.6, n.s. 2.50 2.57^\*^

Cystatin-B 0.50 0.40 0.36^\*^ 0.80, n.s.

Cystatin-C 0.80, n.s. 0.42^\*^ 0.37^\*^ 0.47^\*^

Defender against cell death 0.48 0.43 0.36^\*^ 0.42^\*^

Destrin 0.37^\*^ 0.40^\*^ 0.38^\*^ 0.90, n.s.

EGF-like transmembrane protein 2.50^\*^ 2.62^\*^ 2.31^\*^ 3.22^\*^

Etoposide-induced gene 2.4 0.50 0.49 0.80, n.s. 0.48

Expressed in metastasis cells 1.2, n.s. 1.6, n.s. 1.7, n.s. 2.8^\*^

FGF-BP 1 0.21^\*^ 0.49 0.7, n.s. 0.8, n.s.

FGF intracellular binding prot. 1.4, n.s. 1.5, n.s. 1.3, n.s. 2.19^\*^

Fibronectin-1 2.14 0.36^\*^ 0.43^\*^ 0.49

Fyn proto-oncogene 2.66^\*^ 0.37 0.52, n.s. 1.04, n.s.

Glutamine synthetase 2.10 2.18 2.33^\*^ 2.48^\*^

Glutatione S-Transferase-alpha-4 2.18^\*^ 2.70^\*^ 2.1^\*^ 3.04^\*^

Glypican-4 0.47^\*^ 0.49^\*^ 0.50^\*^ 0.35^\*^

Growth arrest DNA dam.45-j 2.48 2.00 1.60, n.s. 1.3, n.s.

Heparan-S sulphotransferase 1.42, n.s. 0.89, n.s. 0.90, n.s. 2.20^\*^

IGFreceptor II 3.35^\*^ 2.05^\*^ 2.10^\*^ 2.20^\*^

IGF-BP3 2.3^\*^ 3.04^\*^ 11.1^\*^ 6.53^\*^

INF-a responsive gene 15kDA 2.09^\*^ 2.01^\*^ 2.14^\*^ 2.20^\*^

Interferon-inducible GTPase 10.22^\*^ 2.17^\*^ 2.12^\*^ 2.30^\*^

Keratin complex-2 basic 19 1.35, n.s. 2.10 3.32 1.70, n.s.

Keratin comp. 2 basic gene 4 2.34 2.58 2.09 4.3^\*^

L1 cell adhesion molecule 1.57, n.s. 1.81, n.s. 2.32^\*^ 2.34^\*^

Laminin-beta 2 0.50 0.46 0.38^\*^ 0.41

Large tumor suppressor 2 0.8, n.s. 0.32^\*^ 0.49 0.41^\*^

Lymphocyte antigen-6 (Loc. D) 2.30^\*^ 3.27^\*^ 7.42^\*^ 9.2^\*^

Metallothionein-1 0.29 0.38^\*^ 0.17^\*^ 0.16^\*^

Metastasis associated gene 1.05, n.s. 1.07, n.s. 1.5, n.s. 3.1^\*^

MMP-2 2.01^\*^ 2.35^\*^ 3.09^\*^ 3.53^\*^

Myeloid differ. Gene 116 4.01^\*^ 2.33^\*^ 1.82, n.s. 4.46^\*^

Myoxivirus resistance-2 2.48^\*^ 1.25, n.s. 1.09, n.s. 1.80, n.s.

Nexin (clade E) 0.12^\*^ 0.11^\*^ 0.08^\*^ 0.08^\*^

Notch gene 4 0.43 0.50 0.46^\*^ 0.47^\*^

P21-activated kinase-1 0.37^\*^ 0.48^\*^ 0.46^\*^ 0.43^\*^

Peripherin 0.47^\*^ 0.43^\*^ 0.48^\*^ 0.38^\*^

Peroxisome P-A receptor 0.87, n.s. 1.33, n.s. 1.07, n.s. 3.12^\*^

Pinin 2.44^\*^ 2.38^\*^ 2.18^\*^ 2.19^\*^

Procollagen-5, alpha-2 0.25^\*^ 0.17^\*^ 0.33^\*^ 0.13^\*^

Profilin-2 3.26^\*^ 1.20, n.s. 0.74, n.s. 1.29, n.s.

RAB-2 0.48^\*^ 0.47^\*^ 0.43^\*^ 0.51

RAB-6 interacting protein 0.38^\*^ 0.30^\*^ 0.34^\*^ 0.43^\*^

RAB-9 0.49 0.43^\*^ 0.45 0.48

RAB-18 0.30^\*^ 0.48^\*^ 0.42 0.43

RAB-25 2.29^\*^ 3.39^\*^ 2.78^\*^ 3.95^\*^

Reticulocalbin 2.80^\*^ 3.39^\*^ 2.78^\*^ 3.95^\*^

Retinoic acid early transcript 2.01 2.32^\*^ 3.28^\*^ 3.9^\*^

Retinol BP 1 cellular 2.90^\*^ 2.83^\*^ 2.04^\*^ 3.64^\*^

S-100 calcium BP-A11 0.45 0.42^\*^ 0.47^\*^ 0.39^\*^

Selenoprotein P, plasma 1 0.22^\*^ 0.13^\*^ 0.12^\*^ 0.27^\*^

Sialyltransferease-8 3.33^\*^ 1.23, n.s. 1.32, n.s. 0.74, n.s.

Tensin 0.43^\*^ 0.41 0.43^\*^ 0.33^\*^

TGFB-RIII 0.48 0.47 0.45 0.45

TGFB induced transcript 1 0.32^\*^ 0.45 0.50 0.47^\*^

TIMP-2 0.28^\*^ 0.29^\*^ 0.34^\*^ 0.40^\*^

TIMP-3 2.16 2.03 2.30 2.01^\*^

TNF ligand, member 19 2.32 2.18 2.08 3.10^\*^

TNFalpha-induced protein 2 2.18^\*^ 2.12^\*^ 5.23^\*^ 5.97^\*^

Transcription EF-A 2.72^\*^ 2.05 2.33^\*^ 1.7, n.s.

Transcription factor-4 3.88^\*^ 1.7, n.s. 1.5, n.s. 1.2, n.s.

Transglutaminase 2 0.33^\*^ 0.37^\*^ 0.30^\*^ 0.28^\*^

Tropomyosin-4 0.31^\*^ 0.45^\*^ 0.40 0.38^\*^

Tumor assoc. signal transd-2 1.7, n.s. 2.04 2.33^\*^ 2.48^\*^

Thymidylate kinase LSP-inducib. 8.14^\*^ 1.35, n.s. 0.80, n.s. 1.42,
n.s.

Ubiquitin-specific protease 18 2.87^\*^ 1.7, n.s. 1.8, n.s. 2.99^\*^