[Questions]{.underline}

[Testing for Fumonisin]{.underline}

**A**. **[General Questions]{.underline}**

1.  Fumonisin testing service on processed grain products is provided
    under the authority of the United States Grain Standards Act
    (USGSA).

> \(a\) True (b) False

2\. Fumonisins are environmental toxins produced by molds that grow on
agricultural commodities in the field or during storage.

> \(a\) True (b) False

3\. Fumonisin testing service on corn is provided as a factor under the
authority of the United States Grain Standards Act (USGSA).

(a) grade determining (c) optional

(b) special (d) official criteria

4\. Review inspection services for fumonisin are performed on the basis
of a new sample or a file sample in accordance with the regulations.

> \(a\) True (b) False

5\. Fumonisin test results exceeding 2.0 ppm exceed Food and Drug
Administration (FDA) defect action limits and must be reported to the
local FDA office.

> \(a\) True (b) False

6\. Fumonisin test results that are above the test kit\'s established
conformance limits are reported as exceeding the conformance limit
unless a supplemental analysis is performed.

> \(a\) True (b) False

7\. To clean labware (i.e., glassware, funnels, beakers, etc.) used in
the fumonisin testing process, prepare a solution consisting of .

\(a\) Bleach/water (c) Dishwashing liquid/water

\(b\) Distilled water (d) Methanol/water

8\. What is the minimum sample size for fumonisin testing of an
officially sampled railcar?

\(a\) one pound (c) three pounds

\(b\) two pounds (d) ten pounds

9\. What is the minimum sample size for fumonisin testing of an
officially sampled barge?

\(a\) one pound (c) five pounds

\(b\) three pounds (d) ten pounds

10\. Grind the entire sample obtained for fumonisin testing and prepare
two 500-gram subportions from the ground sample. A 500-gram work portion
for original testing services and a 500-gram file sample portion for
review testing.

\(a\) True (b) False

11\. When checking the performance of the sample grinder, a 100-gram
sample portion is ground, then sieved across a standard No. 20 wire
woven sieve. What is the optimum range for particles of coarse and small
grain passing through the No. 20 sieve?

\(a\) 40% - 60% (c) 50% - 75%

\(b\) 50% - 60% (d) 60% - 75%

12\. Upon request of the applicant, separate certificates may be issued
for grade and for fumonisin when both are determined on the same lot.

\(a\) True (b) False

13\. When test results indicate that fumonisin is present at a level of
0.5 ppm or less, certify the results as:

\(a\) less than 0.5 ppm (c) equal to or less than 0.5 ppm

> \(b\) none detected (d) 0.5 ppm

14\. The fumonisin toxin can grow in corn kernels without any outward
signs of mold.

\(a\) True (b) False

15\. Export shiplots are analyzed on a sublot basis for all grains.

\(a\) True (b) False

16\. What is the recommended sample size for testing submitted samples
for fumonisin?

\(a\) one pound (c) two pounds

\(b\) ten pounds (d) any size

**B.** **[Neogen Veratox Fumonisin Test - Specific
Questions]{.underline}**

1\. The extraction solvent used for the Veratox test is made up by using
the ratio of 70 parts of to 30 parts of distilled or deionized water.

\(a\) Acetonitrile (c) Methanol

\(b\) Bleach (d) Acetone

2\. What is the size of ground portion that is used for testing?

\(a\) 50 grams (c) 100 grams

\(b\) 250 grams (d) one pound

3\. In the extraction process, 250 ml of the extraction solvent is added
to the ground portion and blended for minutes.

\(a\) one (c) three

\(b\) five (d) two

4\. Following the extraction process, the sample extract is diluted by
adding of the extract to the pink-labeled, pre-filled sample dilution
bottle.

\(a\) 100µl (c) 50µl

\(b\) 200µl (d) 150µl

5\. To analyze a single sample, use one red-marked mixing well for the
test sample and

wells for controls.

\(a\) one (c) two

\(b\) three (d) five

6\. What is the maximum number of samples that can be tested at one
time, per set of control standards?

\(a\) 19 (c) 21

\(b\) 7 (d) 12

7\. There are two separate incubation periods (referred to as waiting
periods in the instruction) in the testing process. What is the duration
of each waiting period?

\(a\) 1 minute (c) 5 minutes

\(b\) 2 minutes (d) 10 minutes

8\. To rinse the anti-body coated wells, use a wash bottle filled with
distilled/deionized water and shake out. Repeat this step time(s).

\(a\) five (c) three

\(b\) one (d) two

9\. What instrument is used for reading test results?

\(a\) EL-301 microwell reader (c) Stat-Fax Model 321 PLUS microwell
reader

\(b\) RL-100 Fluorometer (d) Hach Spectrophotometer

10\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) ten

\(b\) twenty (d) thirty

11\. Test kits should be refrigerated at temperatures between 36°F and
46°F.

> \(a\) True (b) False

12\. Standard certification procedures require test results between 0.6
ppm and 5.4 ppm to be certified to the nearest whole ppm.

\(a\) True (b) False

**C.** **[r -Biopharm Ridascreen Fumonisin Test - Specific
Questions]{.underline}**

1\. The extraction solvent used for the Ridascreen test is made up by
using the ratio of 70 parts of methanol to 30 parts of water.

\(a\) distilled (c) distilled or deionized

\(b\) deionized (d) tap

2\. What is the size of ground portion that is used for testing?

\(a\) 50 grams (c) 100 grams

\(b\) 250 grams (d) one pound

3\. In the extraction process, 250 ml of the extraction solvent is added
to the ground portion and blended for minutes.

\(a\) one (c) three

\(b\) two (d) five

4\. Following the extraction process, dilute 100 µl of the filtered
extract with ml of distilled or deionized water.

\(a\) 1.3 (c) 2.0

\(b\) 1.5 (d) 2.5

5\. To analyze a single sample, use one red-marked mixing well for the
test sample and

wells for controls.

\(a\) one (c) two

\(b\) three (d) five

6\. What is the maximum number of samples that can be tested at one
time, per set of control standards?

\(a\) 19 (c) 21

\(b\) 7 (d) 12

7\. After adding the sample solution, enzyme conjugate, and fumonisin
antibody to the wells, mix the wells by pipetting the liquid up and down
in the tips 3-4 times, then incubate for

minutes.

\(a\) two (c) five

\(b\) ten (d) fifteen

8\. Which microwell reader is used for reading test results?

\(a\) Biotek EL-301 (c) Stat-Fax Model 303 PLUS

\(b\) Hyperion Model 4027-002 (d) All of the above

9\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) ten

\(b\) twenty (d) thirty

10\. Standard certification procedures require test results exceeding
5.4 ppm to be reported as \> 5.4 unless a supplemental analysis is
performed.

\(a\) True (b) False

11\. Test kits should be refrigerated at temperatures between 34°F and
38°F.

> \(a\) True (b) False

**D.** **[Myco Fumonisin Test - Specific Questions]{.underline}**

1\. The extraction solvent used for the Myco test is made up by using
the ratio of 70 parts of to 30 parts of distilled or deionized water.

\(a\) Acetonitrile (c) Methanol

\(b\) Bleach (d) Acetone

2\. What is the size of ground portion that is used for testing?

\(a\) 50 grams (c) 100 grams

\(b\) 250 grams (d) one pound

3\. In the extraction process, 250 ml of the extraction solvent is added
to the ground portion and blended for minutes.

\(a\) one (c) three

\(b\) five (d) two

4\. The wash Solution is prepared by transferring the contents of the
Wash Concentrate vial to a 500-ml plastic squeeze bottle and adding ml
of distilled or deionized water.

\(a\) 150 (c) 250

\(b\) 200 (d) 475

5\. To analyze a single sample, use one red-marked mixing well for the
test sample and

wells for calibrators (controls).

\(a\) one (c) two

\(b\) three (d) five

6\. What is the maximum number of samples that can be tested at one
time, per set of calibrator (control) standards?

\(a\) 19 (c) 21

\(b\) 7 (d) 12

7\. There are two separate incubation periods (referred to as waiting
periods in the instruction) in the testing process. What is the duration
of each waiting period?

\(a\) 1 minute (c) 5 minutes

\(b\) 2 minutes (d) 10 minutes

8\. To rinse the anti-body coated wells, use a wash bottle filled with
the wash solution and vigorously wash each well by overfilling. Repeat
the vigorous wash more times.

\(a\) five (c) three

\(b\) one (d) two

9\. The microwell reader is approved for reading test results for the
Myco Fumonisin test kit?

\(a\) Biotek EL-301 (c) Stat-Fax Model 303 PLUS

\(b\) Stat-Fax Model 321 PLUS (d) All of the above

10\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) ten

\(b\) twenty (d) thirty

11\. Test kits should be refrigerated at temperatures between 36°F and
46°F.

> \(a\) True (b) False

12\. Standard certification procedures require test results between 0.6
ppm and 5.4 ppm to be certified to the nearest whole ppm.

\(a\) True (b) False

**E**. **[Vicam FumoniTest 200 ™ Specific Questions]{.underline}**

1\. Each fluorometer is equipped with a set of standard vials packaged
three vials per case which must be:

\(a\) Refrigerated when not in use.

\(b\) Stored in a cabinet or drawer in the original case when not in
use.

\(c\) Placed under a fume hood before, during, and after use.

\(d\) None of the above.

2\. During calibration, before placing standard vials in the cuvette
chamber, you should:

\(a\) Wipe each of the vials with a clean cloth or paper wipe.

\(b\) Wash each vial with a bleach solution.

\(c\) Set the calibration value at 20.0 ppb.

\(d\) None of the above.

3\. The extraction method used for FumoniTest includes adding 100 mL of
80/20 methanol/water mixture to 50 grams of ground sample and 5 grams of
salt, then blending the mixture for exactly 1 minute.

\(a\) True (b) False

4.  When checking the calibration of the Series III fluorometer with the
    yellow vial, the value displayed after it has been fully inserted
    into the sample well should be 6.0 ±5 ppm.

> \(a\) True (b) False

5\. The calibration of the fluorometer is checked at least every eight
hours using the yellow vial.

\(a\) True (b) False

6\. The Developer A and B mixture must be prepared .

\(a\) Fresh daily

(b) Weekly

(c) After every test

(d) Every two days.

7\. The developer solution, PBS solution, distilled/deionized water, and
HPLC grade methanol must be checked for background fluorescence.

a\. True (b) False

8\. When performing solution testing, a combination of 1 mL dilute
developer and 1 mL methanol should read \_\_\_\_\_\_\_\_\_ ppm when
inserted into a calibrated fluorometer.

\(a\) 2.0 (c) 0

\(b\) 3.0 (d) 1.0

9\. FumoniTest is a qualitative test method that provides test results
in actual ppm.

\(a\) True (b) False

10\. To calibrate the fluorometer for FumoniTest set the test delay time
to

seconds.

\(a\) 60 (c) 90

\(b\) 45 (d) 240

11\. A small aquarium pump may be used to provide the needed air
pressure for loading, filtering and washing the various extracts through
the affinity column.

\(a\) True (b) False

12\. The PBS solution should pass through the affinity column at a rate
of drops

per second.

\(a\) 1 -- 2 (c) 1.5

\(b\) 2 -- 3 (d) 2

13\. The extraction solvent used in the FumoniTest is a methanol/water
mixture consisting of 80 percent methanol (reagent grade or better) and
20 percent water.

\(a\) True (b) False