**ROBERT E. MANDRELL, Ph.D.**

Research Leader

Produce Safety and Microbiology Research Unit

USDA, ARS, WRRC

800 Buchanan Street

Albany, CA 94710

USA

Tel: 510-559-5829

Fax: 510-559-6165

E-mail: mandrell@pw.usda.gov

#### Education

Ph.D School of Biochemistry, University of Birmingham, UK 1993

B. S. Microbiology, Ohio State University 1970

**RESEARCH AND PROFESSIONAL EXPERIENCE:**

> **1974 to 1983 Walter Reed Army Institute of Research, Department of
> Bacterial Diseases, Washington DC**

-   Studies of antigenicity, immunogenicity and immunochemistry of outer
    membrane molecules of *Neisseria meningitidis*

-   Production and characterization of monoclonal antibodies

-   Development of serotyping system for protein and lipooligosaccharide
    antigens of *Neisseria meningitidis*

-   Production of polysaccharide-protein vaccines

> **1984 to 1992 VA Medical Center, San Francisco, CA**

-   Immunochemistry and biochemistry of lipooligosaccharides of
    *Neisseria meningitidis*, *Neisseria gonorrhoeae, and Haemophlius
    species*

> **1994 to 1996 Oakland Childrens Hospital Research Institute, Oakland,
> CA**

-   Human immune response to bacterial polysaccharide-conjugate vaccines

-   Human repertoire of antibodies to polysaccharides

-   Immunochemistry of *Streptococccus pneumoniae* capsular
    polysaccharides

> **1996 to present USDA, Agricultural Research Service, WRRC Albany,
> CA**

-   Microbial food safety related to fresh produce

-   Biology of *Campylobacter* species related to food

-   Analysis of bacteria by mass spectrometry

## Recent Publications, 1998-2003

1.  **Mandrell, RE** and Wachtel, MR. 1999. Novel detection techniques
    for human pathogens that contaminate poultry. [Current Opinion
    Biotech.
    10(3)**:**273-278](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10361075&dopt=Abstract).
    (Review).

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1.  Brandl, MT, **Mandrell, RE**. 2000. Use of confocal microscopy and
    the green fluorescent protein in ecological studies on *Salmonella*
    on plant surfaces. Scanning 2000*.* 22: 83. (Proceedings).

2.  Wood, DF, **Mandrell, R**, Bates, AH, Yu, PC. 2000.
    Immunolocalization of surface antigens on *Campylobacter* *jejuni*
    using FESEM and a backscatter electron detector. Scanning 2000*.*
    22: 79-80. (Proceedings).

3.  Kimura R, **Mandrell RE**, Galland JC, Hyatt D, Riley LW. 2000.
    Restriction site-specific (RSS)-PCR as a rapid test to detect
    enterohemorrhagic *Escherichia* *coli* O157:H7 strains in
    environmental samples. [Appl Environ Microbiol*.*
    66:2513-2519](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10831431&dopt=Abstract).

4.  Miller WG, Bates AH, Horn ST Brandl MT, Wachtel MR, and **Mandrell
    RE**. 2000. Detection on surfaces and in Caco-2 cells of
    *Campylobacter* *jejuni* cells transformed with new *gfp*, *yfp* and
    *cfp* marker plasmids. [Appl Environ Microbiol.
    66:5426-5436](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11097924&dopt=Abstract).

5.  Campbell J, Mohle-Boetani J, Reporter R, Abbott S, Farrar J, Brandl
    M, **Mandrell, R**, Werner S. 2001. An outbreak of *Salmonella*
    Thompson associated with fresh cilantro. [J Infect Dis.
    183:984-987](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11237818).

6.  Charkowski AO, Sarreal CZ, **Mandrell RE**. 2001. Wrinkled alfalfa
    seeds harbor more aerobic bacteria and are more difficult to
    sanitize than smooth seeds. [J Food Protect. 64:
    1292-1298.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11563502)

7.  Wachtel, MR, Whitehand, LC, **Mandrell, RE**. 2002. Prevalence of
    *Escherichia coli* associated with a cabbage crop inadvertently
    irrigated with partially treated sewage wastewater. [J Food Prot.
    65:471-475.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11899045)

8.  Wachtel, MR, Whitehand, LC, **Mandrell, RE**. 2002. Association of
    *Escherichia coli* O157:H7 with preharvest leaf lettuce upon
    exposure to contaminated irrigation water. [J Food Prot.
    65:18-25.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11808792)

9.  Brandl, MT, **Mandrell, RE**. 2002. Fitness of *Salmonella enterica*
    serovar Thompson in the cilantro phyllosphere. [Appl Environ
    Microbiol.
    68:3614-3621](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12089050&dopt=Abstract).

10. Charkowski, AO, Barak, JD, Sarreal, CZ, **Mandrell, RE**. 2002.
    Differences in growth of *Salmonella enterica* and *Escherichia
    coli* O157:H7 on alfalfa sprouts. [Appl. Environ. Microbiol..
    68:3114-3120.](http://aem.asm.org/cgi/content/abstract/68/6/3114)

11. Friedman, M, Henika, P, **Mandrell, R**. 2002. Bactericidal
    activities of plant essential oils and some of their isolated
    constituents against *Campylobacter jejuni*, *Escherichia coli*,
    *Listeria monocytogenes*, and *Salmonella* *enterica*. [Journal of
    Food Protection.
    65:1545-1560.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12380738)

12. Gorski, L, Palumbo, JD, **Mandrell, RE**. 2003. Attachment of
    *Listeria monocytogenes* to radish tissue is dependent upon
    temperature and flagellar motility. [Appl. Environ.
    Microbiol..69:258-266.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12514003)

13. Palumbo, JD, Borucki, MK, **Mandrell, RE,** and Gorski, LA. 2003.
    Serotyping of *Listeria monocytogenes* by ELISA and identification
    of mixed-serotype cultures by colony immunoblot. [J. Clin.
    Microbiol.
    69.564-571.](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12574247)

14. Cooley, M, Miller, W, **Mandrell, R.** 2003. Colonization of
    *Arabidopsis thaliana* with *Salmonella enterica* or
    enterohemorrhagic *Escherichia coli* O157:H7 and competition by an
    *Enterobacter* *asburiae*. [Appl. Environ. Microbiol.
    69:4915-4926](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12902287).