Ozone Indicator - 2006- New and Returning Eastern Crews

WRITTEN TEST FOR CERTIFICATION

State:\_\_\_\_\_\_\_\_\_ Name:\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
Date:\_\_\_\_\_\_\_\_\_\_ Training Session:\_\_\_\_\_\_\_\_\_\_\_\_

**This test is intended to familiarize the trainee with the ozone
bioindicator plants section of the Field Guide. Answers may be found in
the text of the Field Guide or in one of the appendices. Each response
is worth 1 point.**

1.  **T or F** Ozone is the only regional gaseous air pollutant in the
    U.S. that is frequently measured at concentrations known to be
    harmful to plants.

2.  **T or F** Ozone pollution has been shown to have an adverse effect
    on tree growth and alter tree succession, species composition, and
    pest interactions.

3.  **T or F** We know that ozone causes visible foliar injury to many
    species and that these symptoms include distinct patterns of
    coloration, often associated with accelerated senescence.

4.  **T or F** Ozone can only injure plants if it enters the plant
    leaves through open stomates during the normal process of gas
    exchange.

5.  **T or F** Ozone may have a positive effect on biodiversity by
    weeding out those species unable to tolerate toxic ozone
    concentrations.

6.  **T or F** A useful bioindicator plant may be a tree, a woody shrub,
    or a non-woody herb species as long as it responds to ambient levels
    of ozone pollution with obvious foliar injury symptoms that are easy
    to diagnose.

7.  **T or F** The only field equipment needed to complete the ozone
    indicator procedures is a hand lens.

8.  **T or F** A map of the biomonitoring site must include the location
    of the site relative to some obvious and permanent marker, nearby
    road names and distances, a north arrow, species codes and
    approximate locations of the plants used for ozone injury
    evaluations, the starting point for ozone injury evaluations,
    directions and distances to two major roads and two major towns, and
    geographical coordinates at the starting point or some other obvious
    marker.

9.  **T or F** When crews cannot find 30 plants of two or more species
    to evaluate at a biosite, they must select a second location nearby
    to collect additional data.

10. **T or F** Biosites that consist of more than one location are
    called split plots and have different ozone plot numbers.

11. **T or F** If the plot map leads you to a field now occupied by a
    residential estate, you must find a replacement biosite.

12. **T or F** A biosite moved to a new location is recorded as Ozone
    Sample Kind 1.

13. **T or F S**pecies like blackberry and milkweed can spread
    vegetatively, creating large clumps of genetically similar plants.

14. **T or F** Bioindicator species are measured for height, light
    exposure, and drought tolerance as these influence ozone
    sensitivity.

15. **T or F** Injury amount is estimated based on the number of leaves
    on the plant with ozone injury symptoms relative to the total number
    of leaves on the plant.

16. **T or F** For each plant, **i**njury severity is estimated based on
    the average percent of the leaf area injured on injured leaves.

17. **T or F** If 7 of 30 black cherry trees are symptomatic, then 7
    voucher leaves are collected and placed in the plant press.

18. **T or F** QA crews are exempt from the requirement to collect
    voucher leaf samples when they find ozone injury.

19. **T or F** Leaves with obvious injury symptoms make the best voucher
    samples.

20. **T or F** Sunlight is necessary for the production of the
    purple-red pigmentation that is characteristic of ozone injury.

21. **T or F** Crews do not record amount and severity scores unless
    they are certain the symptoms are caused by ozone.

22. **T or F** The biosite map shows the approximate location of the
    plant species evaluated for ozone injury so that the same
    populations of plants are remeasured every year.

23. **T or F** A biosite with nothing but zero values is not acceptable.

24. **T or F** It is important to record the Datum used to collect GPS
    measurements in the Plot Notes and on the biosite map.

25. **T or F** A non-tallied plant for a given species is one that is
    deliberately skipped during the selection of 30 plants for
    evaluation.

26. **T or F** Supplemental species are used only after 30 plants of 2
    or more species from the list of official bioindicator species have
    been evaluated for ozone injury.

What are the selection criteria for ozone biomonitoring sites? Define a
"best" site in terms of the following site characteristics.

  --------------- -------------------------------------------------------
  27\. Site       
  access:         

  28\. Number of  
  Locations:      

  29\. Size of    
  opening:        

  30\. Number of  
  Species:        

  31\. Number of  
  Plants:         

  32\. Soil       
  conditions:     

  33\. Site       
  disturbance:    
  --------------- -------------------------------------------------------

34\. How do voucher leaf samples from species like white ash that have
compound leaves differ from voucher leaf samples from species like black
cherry that have simple leaves?

What are 4 things you can do to ensure that your leaf samples get to the
National Advisor properly labeled and in good condition?

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