Nadim Majdalani

NIH/NCI

Laboratory of Molecular Biology

37 Convent Drive

Bldg 37, Rm 5132

Bethesda, MD 20892-4264

[E-mail:]{.underline} nadim@nih.gov

[Phone:]{.underline} (301) 451-8788

[FAX:]{.underline} (301) 496-2212

[Date and Place of Birth]{.underline}: March 3, 1964, Beirut, Lebanon

[Nationality]{.underline}: US Citizen

[Home address:]{.underline} 4710 Bethesda Avenue Apt. \# 1001

Bethesda, MD 20814

Tel: (301) 718-6463

**[Education and training]{.underline}**

2003- Staff Scientist. Dr. Susan Gottesman, National Institutes of
Health, National Cancer Institute. Small RNAs as gene regulators in *E.
coli*.

2003. Research Fellow. Dr. Susan Gottesman, National Institutes of
      Health, National Cancer Institute. Isolation and characterization
      of a novel small RNA, RprA.

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2000. Post-doctoral fellow. Dr. Susan Gottesman, National Institutes of
      Health, National Cancer Institute. (Fogarty Visiting fellow
      1995-1997, CRTA post-doctoral fellow 1997- ). Characterization of
      the translational and transcriptional regulation activities of the
      small RNA, DsrA.

1988-1995 Graduate Research Assistant, Dr. Karin Ippen-Ihler, Medical
Microbiology and Immunology, Texas A&M University Health Sciences
Center.

> Thesis: Maturation of F propilin: the roles of the propilin leader
> peptide, host encoded proteins, and the F transfer protein TraQ.
>
> Ph.D. Texas A&M University, 1995.

1986-1988 Graduate Research Assistant, Dr. Allison Ficht, Medical
Biochemistry, Texas A&M University. Isolation and collection of
*Fasciola hepatica* organisms from cattle liver. Screening and
characterization of eggshell antigens.

1982-1985 Bachelor of Science, American University of Beirut.

**[Mentoring and teaching]{.underline}**

Jihanne Benhammou Post-Bac fellow 2005-2006. Undergraduate Scholarship
Program. Mechanism of RprA regulation of RpoS mRNA stability. University
of Texas, Austin. (Currently medical student, UCSF)

Jihanne Benhammou Summer Intern, 2004. Undergraduate Scholarship
Program. Mechanism of RprA regulation of RpoS mRNA stability. Junior,
University of Texas, Austin.

Meredith Moore Intern, 2002-2003 National Institutes of Health.
Characterization of EmrR, a Mar-like gene regulator. Walt Whitman High
School Junior. (Currently Junior Psychology major, Bryn Mawr College).

Brandon Ogbunugafor Undergraduate Scholarship Program. 2000- 2002.
Isolation of targets of RprA activity. Chemistry senior, Howard
University, Washington, D.C and pre-CRTA Fellow. (Recipient of
Fullbright Scholarship for study in Africa, currently MD/PhD student,
Yale University)

David Hernandez Undergraduate Scholarship Program, pre-CRTA Fellow,
1999-2000; National Institutes of Health. Analysis of the promoter of
RprA, a novel small RNA that regulates RpoS translation. Graduate
InterAmerican University, Puerto Rico. (Currently in clinical training,
Puerto Rico)

Kristin St John Intern, 1998-1999; National Institutes of Health.
Characterization of a new RNA with DsrA-like activity. Walt Whitman High
School senior.

Jonathan Murrow Summer intern, 1998; National Institutes of Health.
Characterization of a set of overlapping clones with DsrA-like activity.
Emory University School of Medicine medical student. (Currently,
physician Baltimore, Maryland).

SuAnn Chen Summer intern, 1997; National Institutes of Health. Screening
and isolation of DsrA-like clones from an *E. coli* library. Princeton
University undergraduate.

Anirban Gupta Summer intern, 1996; National Institutes of Health.
Temperature regulation of the small RNA, DsrA. University of Maryland
undergraduate. (Currently, physician).

Catherine Ward Honors program senior project; 1993-1994; Texas A&M
University Dept. of Biochemistry. Winner of best Biochemical Thesis
Award, Honors Program in Biochemistry. Construction of TraQ-PhoA fusions
to determine the membrane topology of TraQ. (Currently, physician Akron
Children's Hospital Genetics Dept.)

Trang Nguyen Summer intern, 1994; Texas A&M University. Characterization
of TraA pilin. Texas A&M School of Medicine.

Justin Cetas Summer intern; 1993; Texas A&M University. Over-expression
and purification of TraQ. University of Arizona. (Currently, MD/PhD,
neurosurgery faculty Oregon Health and Science University).

1988-1989 Teaching Assistant, Introductory Biology, Texas A&M
University.

1986-1987 Teaching Assistant, Introductory Biology, Texas A&M
University.

1985-1986 Teaching Assistant, Botany, American University of Beirut.

**[Reviewing]{.underline}**

Proceedings of the National Academy of Sciences

Journal of Bacteriology

American Journal of PharmacoGenomics.

**[Committees and Activities]{.underline}**

Member American Society for Microbiology

NIH Radiation Safety Authorized User

NIH Emergency Evacuation coordinator

**[Awards]{.underline}**

NIH Fellows Award for Research Excellence 1999. National Institutes of
Health, Bethesda, MD. October 19, 1998.

1st Prize Award poster contest. Graduate Research Symposium. Texas A&M
University College of Medicine, College Station, TX. March 9, 1995.

**[Invited Research Seminars]{.underline}**

Physiological roles for regulatory small RNAs in *E. coli*. American
University of Beirut, Beirut, Lebanon. January 24, 2007

Small RNA regulation of gene expression in *E. coli*. The Free
University of Brussels, Brussels, Belgium. October 11, 2006

Gene Regulation by small regulatory RNAs in *E. coli*. The Pasteur
Institute, Paris, France. October 9, 2006

Non-coding RNA regulation of gene expression in *E. coli*. Rudbeck
Laboratory, University of Uppsala, Sweden. October 5, 2006

Regulation of the small RNA modulators of a developmental switch in *E.
coli*. NHBLI, National Institutes of Health, Bethesda, MD, April 22,
2005.

Regulation by small RNAs; what we have learned from *E. coli*. Biology
Dept., American University of Beirut, Beirut, Lebanon, January 7, 2005.

Stealth regulation: the use of small RNAs in gene regulation in *E.
coli*. Biology Dept., American University of Beirut, Beirut, Lebanon,
January 3, 2003.

Régulation de l\'expression génétique par les petits ARNs: de
l\'antisens à l\'interférence. Ce que les bactéries nous ont appris.
Dept. of Microbiology, IBMM Free University of Brussels, Gosselies,
Belgium, December 16, 2002.

Stealth regulation: the use of small RNAs in gene regulation in *E.
coli*. Dept. of Biochemistry, IBPC, Paris, France, December 15, 2002.

Translational regulation of RpoS in response to the environment is
mediated by at least two small regulatory RNAs: DsrA and RprA. Division
of Genetics, Texas A&M University, College Station, TX, January 17,
2002.

The response of *E. coli* to certain environmental conditions is
mediated by two small regulatory RNAs: DsrA and RprA. Biology
Department, The American University of Beirut, Beirut, Lebanon. July 19,
2001.

Environmental sensing of RpoS is mediated by DsrA and RprA: the mode of
action and the regulation of synthesis of RprA. The Lambda lunch series,
Procaryote Interest Group, National Institutes of Health, June 28, 2001.

The translational regulation of RpoS in response to some environmental
signals is mediated by two small RNAs: DsrA and RprA. FASEB Summer
Research Conference on Post-Transcriptional Control of Gene Expression;
Copper Mountain, CO, July 19, 2000.

Functional small RNAs: DsrA and RprA as activators of RpoS-lacZ
translation. The Lambda lunch series, Procaryote Interest Group,
National Institutes of Health, December 2, 1999.

DsrA RNA regulates RpoS translation and acts as an antisilencer of
transcription of H-NS-silenced genes. The Pasteur Institute, Paris,
France. July 9, 1998.

DsrA RNA regulates the translation of the stationary-phase sigma factor
RpoS by an anti-antisense mechanism. The Free University of Brussels,
Belgium, June 22, 1998.

Transcrptional and translational regulation functions of the small RNA,
DsrA. The Lambda lunch series, Procaryotic interest group, National
Institutes of Health, May 1, 1997.

Maturation of F-pilin. The Lambda lunch series, Procaryotic interest
group, National Institutes of Health, December 9, 1995.

**[Research Abstracts]{.underline}**

Majdalani, N. and S. Gottesman. Signaling through the Rcs phosphorelay.
Microbial Genetics and Genomics III. Moab, UT, May 6-9, 2005.

Majdalani, N., and S. Gottesman. Signal transduction in the Rcs
phospho-relay pathway. Molecular Genetics of Bateria and Phages.
Madison, WI, Aug. 24-29, 2004.

Majdalani, N, M. Heck, D. Hernandez D., S. Gottesman and V. Stout.
Components of the RcsB/RcsC phospho-relay pathway. Amer. Soc. for
Microbiology 103^rd^ General meeting. Washington D.C., May 18-22, 2003.

Majdalani, N, D. Hernandez and S. Gottesman. Regulation and mode of
action of RprA, a small RNA that stimulates RpoS translation. Amer. Soc.
for Microbiology 102^nd^ General meeting. Salt Lake City, UT, May 19-23,
2002.

Majdalani, N, D. Hernandez and S. Gottesman. Environmental signals
regulate RpoS expression via two small RNAs: DsrA and RprA. Genetics and
Genomics of Microbial Systems. Fontevraud l'Abbayye, France, April
12-15, 2002.

Majdalani, N, D. Hernandez and S. Gottesman. The synthesis of the RpoS
regulator, RprA, is controlled by RcsB and the capsule two component
regulation system RcsB/C. Amer. Soc. Microbiology 101st General meeting,
Orlando, FL, May 20-24, 2001.

Majdalani, N, D. Sledjeski, F. Repoila, C. Cunning, T. Elliott, SA.
Chen, J Morrow, K. St John, D. Hernandez and S. Gottesman. The
translational regulation of RpoS in response to some environmental
signals is mediated by two small RNAs: DsrA and RprA. FASEB Summer
Research Conference on Post-Transcriptional Control of Gene Expression;
Copper Mountain, CO, July 16-21, 2000.

Majdalani N, J. Murrow, S. Chen, K. St John and Gottesman S. Functional
small RNAs: a potential small RNA that can promote the expression of an
RpoS-lacZ translational fusion. Amer. Soc. Microbiology 99th General
meeting, Chicago, IL, May 30-June 3, 1999.

Majdalani N, C. Cunning, T. Elliott, D. Sledjeski, K. St John, SA. Chen,
J. Murrow and Gottesman S. Small regulatory RNAs: the DsrA and RprA
stories. Molecular genetics of microbial systems, Amalfi, Italy, April
9-12, 1999.

Majdalani N, Cunning C, Elliott T and Gottesman S. Regulation of RpoS
translation by DsrA RNA: an anti-antisense model. NIH Research Festival,
Bethesda, MD, October 6-9, 1998.

Majdalani N, Moore D, Mannewannakul S, Ward C and Ippen-Ihler K. Partial
characterization of the TraQ protein of the conjugative plasmid F.
Fallen Leaf Lake Conference on Unifying Themes Among Protein and Nucleic
Acid Transporters of Bacteria. Fallen Leaf Lake, South Lake Tahoe,
California, September 17-20, 1998.

Majdalani N, Sledjeski D, Cunning C, Elliott T and Gottesman S.
Independent transcriptional and translational effects of a small RNA.
Cold Spring Harbor Symposium on Mechanisms of transcription. Cold Spring
Harbor, New York, June 3-8, 1998.

Majdalani N, Cunning C, Elliott T and Gottesman S. Regulation of RpoS
translation by DsrA RNA: an anti-antisense model. Amer. Soc.
Microbiology 98th General meeting, Atlanta, Ga, May 17-21, 1998.

Majdalani, Sledjeski and Gottesman. Independent regulation by the small
RNA, DsrA, of H-NS silencing of transcription and RpoS translation in
*E. coli*. Keystone Symposia on Molecular and Cellular Biology. The
Bacterial Chromosomes meeting, Santa Fe, New Mexico, February 6-12,
1998.

Majdalani, Sledjeski and Gottesman. The small RNA, DsrA, regulates
translation of the stationary phase sigma factor RpoS and transcription
of H-NS-silenced genes by different pathways. Amer. Soc. Microbiology
97th General Meeting, Miami, Fla May 4-8, 1997.

Majdalani and Ippen-Ihler. Translocation of F-pilin. Genetics of
regulation, protein export and cell division. The Jonathan Beckwith
Reunion Meeting, Woods Hole, MA, April 19-21, 1996.

Majdalani and Ippen-Ihler. Maturation of F-pilin is sec-independent,
TraQ-dependent, and is inhibited by a temperature-sensitive mutation
affecting leader peptidase. Cold Spring Harbor Symposium on Quantitative
Biology. Protein kinesis: the dynamics of protein trafficking and
stability. Cold Spring Harbor Laboratory, NY. May 31-June 7, 1995.

Majdalani, Maneewannakul, Moore and Ippen-Ihler. Processing of the
F-pilin precursor, propilin. Amer. Soc. Microbiol. 95th General Meeting,
Washington, DC. May 21-25, 1995.

Majdalani and Ippen-Ihler. Maturation of F-pilin. Graduate Research
Symposium. Texas A&M University College of Medicine, College Station,
TX. March 9-10, 1995.

Majdalani and Ippen-Ihler. Maturation of F-pilin is secA-independent but
is inhibited by a temperature-sensitive mutation affecting LepB. Amer.
Soc. Microbiol. 93rd General Meeting, Atlanta, Ga. May 16-20, 1993.

Majdalani and Ippen-Ihler. Maturation of F-pilin is SecA-independent but
is inhibited by a temperature-sensitive mutation affecting LepB. The
Beckwith Reunion Meeting, Woods Hole, MA; March 19-21, 1993.

**[Publications]{.underline}**

Benhammou, J., Majdalani, N. and S. Gottesman. Positively regulated
targets of the small RNA, RprA. (2007). (Manuscript in preparation).

Majdalani, N. and S. Gottesman. (2007). Signaling from RcsF through
RcsC. (Manuscript in preparation).

Majdalani, N. and S. Gottesman. (2007). Genetic Dissection of signaling
through the Rcs phosphorelay. Methods of Enzymology. **423**: 349-362

Gottesman, S., N. Majdalani J. Benhammou, C. McCullen, M. Guillier, K.
Thompson, C. Vanderpool, N. Sowa, D. FitzGerald and Peter FitzGerald.
(2007). Small RNA Regulators and the Bacterial Response to Stress. Cold
Spring Harbor Symposia on Quantitative Biology. **71**: 1-11.

Majdalani, N., M. Heck, V. Stout and S. Gottesman. (2005). Role of RcsF
in signaling to the Rcs phosphorelay in *E*scherichia *coli*. J. Bact.
**187**: 6770-6778.

Majdalani, N. and S. Gottesman. (2005). The Rcs Phosphorelay: a complex
signal transduction system. Annual Reviews in Microbiology.
**59**:379-405.

Majdalani, N., C. Vanderpool and S. Gottesman. (2005) Bacterial small
RNA regulators. Critical Reviews in Biochemistry and Molecular Biology.
**40**: 93-113.

Majdalani, N. and S. Gottesman. (2004). Below our radar: Small RNAs are
stealthy regulators of gene expression. CCR Frontiers in Science (3)
4-5.

Majdalani, N., F. Repoila and Gottesman, S. (2003). Small non-coding
RNAs, coordinators of adaptation processes in *Escherichia* *coli*: The
RpoS paradigm. Mol. Microbiol. **48**: 855-861.

Massé, E., N. Majdalani and Gottesman, S. (2003). Regulatory roles for
small RNAs in Bacteria. Current Opinion in Microbiology **6**: 120-124.

Majdalani N, D. Hernandez and Gottesman, S. (2002). Regulation and mode
of action of the second small RNA activator of RpoS translation, RprA.
Mol. Microbiol **46**: 813-826.

Gottesman S, Storz G, Rosenow C, Majdalani N, Repoila F, Wassarman K.
(2001). Small RNA Regulators of Translation: Mechanisms of Action and
Approaches for Identifying New Small RNAs. Cold Spring Harbor Symposia
on Quantitative Biology: The Ribosome, **66**:353-362.

Majdalani N, Chen SA, Murrow J, St John K, Gottesman S. (2001).
Regulation of RpoS by a novel small RNA: the characterization of RprA.
Mol. Microbiol. **39**: 1382-1394.

Majdalani N, Sledjeski D, Cunning C, Elliott T, Gottesman S (1998). DsrA
regulates translation of RpoS by an anti-antisense mechanism,
independent of its action as an antisilencer of transcription. Proc.
Natl. Acad. Sci. USA **95**: 12462-12467.

Gottesman S, Majdalani N, Sledjeski D (1997). Transcriptional and
translational control by DsrA, a small RNA. FASEB JOURNAL 11: (9) 75 ,
Suppl. S JUL 31.

Majdalani N, Maneewannakul S, Moore D, Ippen-Ihler K (1996). Role of the
propilin leader peptide in the maturation of F-pilin. J. Bact. **178**:
3748-3754.

Majdalani N, Moore D, Ippen-Ihler K (1996). Membrane insertion of the
F-pilin subunit is Sec-independent but requires leader peptidase B and
the proton motive force. J. Bact. **178**: 3742-3747.

Majdalani N, Ward C, Moore D, Ippen-Ihler K. Topology of the F-transfer
protein TraQ (manuscript in preparation).

Majdalani N, Moore D, Maneewannakul S, Ippen-Ihler K. The F-transfer
protein TraQ acts as a chaperone to promote translocation of the
F-transfer pilin subunit (manuscript in preparation).