NOAA Ocean Exploration

Life on the Edge Expedition 2003

Quick Look Report: Mission Summary

Kenneth J. Sulak, Chief Scientist

16-27 August 2003

Expedition Title: Life on the Edge

Principle Investigators and Institutions:

1)  Dr. Steve W. Ross, North Carolina National Estuarine Research
    Reserve, Wilmington, NC (PI)

2)  Dr. Kenneth J. Sulak, U.S. Geological Survey, Gainesville, FL
    (Co-PI)

3)  Dr. Martha Nizinski, NOAA Systematics Laboratory, Washington, DC
    (Co-PI)

4)  Elizabeth Baird, North Carolina State Museum, Raleigh, NC (Co-PI)

Project Title: Exploration of Unique "Reef" Outer Shelf and Slope
Habitats off the Carolinas (South Atlantic Bight).

Expedition Dates and Itinerary: Refer to Tables 1 & 2, and Appendix I.

Chief Scientist and Institution: Dr. Kenneth J. Sulak, U.S. Geological
Survey, Biological Resources, Center for Aquatic Resource Studies,
Gainesville, FL

Co-Sponsors, Partners, Participating Organizations:

1)  NOAA Office of Ocean Exploration

2)  NOAA Systematics Laboratory, at Smithsonian Institution

3)  North Carolina State Museum (NCSM)

4)  University of North Carolina at Wilmington (UNCCW)

5)  U.S. Geological Survey (USGS)

6)  North Carolina National Estuarine Research Reserve (NCNEER)

7)  National Undersea Research Center, University of North
    Carolina-Wilmington (NURC)

8)  NAPRO Communications, Raleigh, NC (Art Howard)

9)  University of Miami (Marine Technician Capability)

10) University of Florida

Scientific Crew:

1)  Dr. Kenneth J. Sulak, USGS, Fish Biologist

2)  Dr. Martha Nizinski, NOAA Fisheries, Decapod Biologist

3)  Dr. R. Allen Brooks, USGS, Invertebrate Ecologist

4)  Elizabeth Baird, North Carolina State Museum, Public Education and
    Outreach Coordinator

5)  Doni Angell, North Carolina Public Educator of Excellence

6)  Ann Marie Necaise, NCNEER, Fisheries Biologist

7)  Tara Casazza, NCNEEER, Fisheries Biologist & UNCW graduate student

8)  Andrea Quattrini, UNCW Fisheries Biologist

9)  Jacquie Ott, NCNEER Data Manager

10) Paula May, NCNEER Water Quality Technician

11) Art Howard, NAPRO Communications, High Definition Videographer

12) Michael Randall, USGS Contract Fisheries Biologist

13) James Berg, USGS Contract Fisheries Biologist & University of
    Florida graduate student

14) Andy Quaid, USGS Contract Marine Chemist

15) Frank Chidsey, University of Miami Marine Technician

NOAA Mission Coordination Crew:

1)  Jeremy Potter, Ocean Exploration (OE) Mission Coordinator

2)  Margot Bohan, OE Web Coordinator

3)  Kristin Heron, OE Data Manager

Land-Based Logistics Support:

1)  Jana Miller, USGS Contract Web Communications Interface

Research Vessel: RV "Seward Johnson", Harbor Branch Oceanographic
Institution.

Embarked Vehicles, Sensors, Logistics: Research Submersible DSRV
"JSL-2"; JSL-2 Seabird CTD; USGS Underwater Digital Still Camera System;
NURC Underwater Digital Camera Control and Communications; University of
Miami Acoustic Doppler Current Profiler & surface water quality sensory
suite; NCNEER Seabird CTD; NCNEER and USGS sampling nets and
peripherals; NCNEER mapping software.

Geographic Area of Operations: Atlantic Ocean, South Atlantic Bight,
Carolinas Outer Shelf and Slope. Refer to Figs. 1, 2.

Summary of Cruise Objectives:

1)  Locate, profile and define poorly-known deep "reef" habitats,
    including deep *Lophelia* coral bank habitats.

2)  Obtain qualitative and quantitative dates to describe community
    structure for all study sites (including the whole water column
    relative to a holistic concept of community structure and function).

3)  Collect selected specimens for taxonomic identification, vouchering
    and photographic documentation.

4)  Collect tissue samples from representative dominant species for
    stable isotope analyses of food habits.

5)  Employ underwater digital still and video cameras to document the
    fauna, species interactions and habitat affinities.

6)  Examine the use of hard bottom and adjacent habitats as nursery
    areas for economically important species.

7)  Compare North Carolina outer shelf hard ground fish communities with
    those found at similar depths in the Gulf of Mexico.

8)  Collect faunal baseline data within the proposed North Carolina
    Marine Protected Area (MPA) to evaluate boundaries and candidate
    habitats.

9)  Construct a trophic model (food habits, food web) for deep coral
    bank communities.

10) Classify deep (\>250m) reef fish species for degree of habitat
    affinity or obligation.

11) Provide education and outreach products to a variety of audiences
    during and following the at-sea mission.

12) Provide at-sea educator experience and real-time sea communication
    to the public.

13) Obtain High Definition video documentation of mission for public
    outreach utilization.

14) Accomplish at-sea media day.

15) Confirm true nature of the seafloor archeological artifact known at
    the "Snowy Wreck" within the proposed MPA.

Summary of Cruise Operations: Refer to Tables 1 & 2, Figs. 1 & 2,
Appendix I.

Milestones Achieved:

With the notable exception of no bottom trawling and minor exceptions
noted below, all at-sea components of the listed objectives were
successfully accomplished. At-sea activities will be followed by
extensive data compilation and analysis in the laboratories of the
various mission scientists. It is anticipated that several scientific
manuscripts, oral reports at scientific meetings and workshops, and
graduate theses will be completed or augmented based on mission results.

Overall, submersible and surface investigations were undertaken at four
primary target sites (Fig. 1) along the outer shelf and slope of the
Carolinas. Undersea operations conducted comprised 17 submersible dives
plus one ROV dive (Table 1, Fig. 2), providing 34 slots for 13
scientific observers representing several state and federal agencies,
one public museum, two universities, and one multi-media productions
company. Scientific operations conducted from the surface comprised 50
surface sampling stations, plus 21 midwater sampling stations using
several gear types (Table 2, Fig. 2, Appendix I). Samples will be used
to analyze trophic linkages in the outer shelf surface to bottom
ecosystem. Tissue samples from 20 species (287 samples) were obtained
for stable isotope analyses in relation to ongoing trophodynamic
studies. Additional surface collections of significance to ongoing
University of North Carolina at Wilmington graduate student research
were accomplished. Each net deployment was accompanied by a Seabird CTD
plot (e.g., Fig. 3) A large number of fathometer surveys were
undertaken, with resulting digital image files (e.g., Fig. 4) serving to
pinpoint areas of undersea topography documenting deep coral reefs. A
previously poorly investigated area, the Stetson *Lophelia* bank area
(Fig. 1) was surveyed, with submersible explorations accomplished.

Approximately 600 digital still images from underwater *in situ*
photography were obtained to document the fish and invertebrate fauna,
and reef habitats. Digital layout images documenting over 40 species
were obtained from sampled specimens prepared in the laboratory. Data
was systematically recorded as per standard forms (Appendix 2). All data
were electronically registered in digital format with multiple backup
copies. All submersible videotapes were duplicated onto DVD digital
format, with three copies produced for archival and analysis at each of
three PI scientific institutions (USGS, UNCW, NOAA Fisheries). All
specimens collected will be archived at the same institutions, pending
analysis. Final archival of voucher specimens will be at the Smithsonian
Institution, the North Carolina State Museum, and the Florida Museum of
Natural History.

A deployment of the unmanned ROV video platform confirmed for the first
time that the seafloor target known as the "Snowy Wreck" is indeed a
shipwreck of a steel-hulled, 120-ft long vessel that is largely intact.
A specimen of the exotic invasive species *Pterois* sp.(Indo-Pacific
lionfish) was collected to document this species in the Atlantic.
Underwater submersible faunal observations before and after arrival of a
commercial fisheries boat at the MPA-2 site documented a dramatic
removal of large economic species during the time slot of the mission.

Public education and outreach activities accomplished are summarized
below. A media visit day was accomplished at sea on 20 August (see
below). Additional post-cruise website and outreach activities will
continue via the home institutions of the mission co-PIs.

OBJECTIVES NOT ACHIEVED:

Deficiencies in accomplishing all objectives in full were due to three
main limitations: 1) First and most significantly, the RV "Seward
Johnson" was not properly or fully outfitted for trawling and bottom
survey activities specified in the mission proposal and study plan. The
RV lacked an oceanographic winch capable of deploying bottom trawls. The
available winch was fitted with only 0.25" diameter wire, and with only
ca 800m of wire. No meter wheel was available for either the stern
"A"-frame or the starboard gallows. Thus, planned bottom sampling
activities integral to holistic water column studies could not be
accomplished, and midwater sampling could not be controlled in terms of
target depth. 2) The USGS digital camera had previously successfully
been employed on several ROV missions, but was in its initial deployment
aboard JSL. Moderate success was achieved in using this camera since the
mating to the JSL-2 encountered some communication and image downloading
difficulties. Furthermore, use of the computer screen driven controller
proved awkward in the confined space of the JSL-2 sphere. USGS and NURC
will work to refine the controller system for future submersible
missions. 3) Events ashore necessitated a late switch in the mission
chief scientist, resulting in less effective planning and coordination
immediately preceding the mission, and a late re-arrangement in the
itinerary for scientist participation in sub dives. This led to a less
well-organized mission than accomplished in previous years.

One full dive day was lost due to limiting weather and sea conditions,
but a substitute ROV dive was successfully undertaken.

# Sample Log Data Forms: Refer to Appendix II

Metadata Summary of Digital Data Collected:

All audio, visual, and log sheet data have been captured digitally
during the mission. Each PI will retain a copy. Data file types include
the following:

1)  Station data and specimen data files in Excel spreadsheet format.

2)  Isotope data files in Excel spreadsheet format; photolog data files
    in Excel spreadsheet format.

3)  Digital audio files recording the in-sub comments and observations
    of both the bow and stern compartment scientists.

4)  Digital image files in .jpg and/or .tif format from the USGS digital
    still camera.

5)  Digital layout image files in .jpg and/or .tif format from the
    laboratory digital camera stand.

6)  Digital image files captured in bitmap format from the RV Seward
    Johnson wheelhouse fathometer.

7)  Digital ArcView shape files documenting coordinates and depths of
    sampling and surveying operations.

8)  Digital ADCP current vector data files from the University of Miami
    Marine Technician.

9)  Digital Seabird CTD data files from both the JSL-2 (submersible
    dives) and from deployed net gear (NCNEER Seabird system).

DATA MANAGEMENT SYNOPSIS (ANDY QUAID):

Multiple types of digital data were collected as indicated above. The
data was compiled onto DVD-Rom disks with a capacity of 4.7 Gigabytes
each. The data were separated into two parts. The first part comprises
the physical and chemical data collected by the ship's systems, and *in
situ* devices. The second part comprises the digital photographs taken
on board the ship and with the underwater digital camera on board the
Johnson Sea Link-2 submersible (JSL-2). Along with the digital
photographs taken onboard the JSL-2, digital video was recorded for each
dive. Table 3. summarizes the digital data collected, including file
types and sizes.

The sea surface and atmospheric data consists of physical and some
chemical properties of the water and atmosphere during the cruise.
Physical data included temperature and conductivity (calculated to
salinity). Chemical data was limited to chlorophyll content of the
surface water, monitored by a fluorometer. Atmospheric data included,
temperature, wind speed and direction (apparent and true),
precipitation, and light levels of photosynthetic active radiation,
visible light, and ultra-violet light. Water depth was also monitored by
the ship's depth-sounder. Winch-block data was also saved in the ship's
data archive. The winch-block data recorded the amount of wire deployed
for the netting operations. The data collected by the R/V Seward Johnson
were saved in a data file (265 mega-bytes). These data were monitored
and collected by the University of Miami Marine Technology Group.

The netting operations were monitored for depth and temperature using a
Seabird SBE39 data sonde. The sonde records depth using a pressure
strain gauge; temperature was monitored by a thermistor. The device was
used to monitor the depth the net reached during deployment. Data
comprised 877 kilobytes.

The acoustic Doppler current profiler monitors current vectors beneath
the ship. This system monitors the speed and direction of the currents,
both at the surface and at depth. The device used was an Ocean Surveyor
(38 kHz). Data may be used for logistic purposes for submersible dives,
and for monitoring ambient reef currents. This data package was 3.5
giga-bytes in size.

Digital Voice Recordings were taken during the submersible dives, both
in the forward and rear compartments. The recordings were taken on Sony
digital voice recorders. The voice recordings will be analyzed for
future missions. The voice recording data occupy 1.0 giga-bytes of
memory.

The Fathometer screen captures were taken to survey and document
selected dive sites during the cruise. Data were in bitmap format, and
contained information on the bottom type, depth, and GPS location; data
occupied 500 mega-bytes of memory.

The JSL-2 CTD data were used to monitor water quality parameters during
the submersible dives, including conductivity, temperature, depth,
oxygen concentration, and pH. The conductivity, temperature and depth
data can be used to derive other data such as salinity and density, and
to show differences in water masses. Data were saved in a text format
occupying 4.5 mega-bytes.

Art Howard from Napro Communications recorded high definition video of
deck operations and submersible operations. A total of 10 hours of high
definition video was taken. Digital video onboard the JSL-2 was taken
for all submersible operations. This video will be used for taxonomic
identification, and habitat type determination. The recordings totaled
approximately 60 hours. The digital still images from the JSL-2 dives
were taken using a Nikon Cool Pix 995, in an underwater housing. These
images were in a jpeg (.jpg) format. The total memory size of all images
was 729 mega-bytes. Light Table (or Layout) photographs were taken using
a Nikon Cool Pix 4500. These photos were recorded in jpeg and tiff
formats. The total size of the unaltered images was 1.97 mega-bytes. The
total may be reduced by removal of duplicate images.

Position data for the JSL-2 was recorded to allow for reef analyses, and
to link to the CTD data. The data can also be compared to the acoustic
Doppler current profiler for logistic planning of future submersible
operations. Data in text file format totaled nearly 50 mega-bytes of
memory.

Summary of Outreach Activities (Liz Baird):

Education and outreach activities contributed to the mission as follows:

The North Carolina Museum of Natural Sciences' Web site hosted the "Life
on the Edge" virtual trek. This included daily journal entries written
by Liz Baird and Doni Angell, school curriculum materials, GIS maps and
images from the cruise. Additionally, more than 50 questions from across
North Carolina and the United States were posed, facilitated via USGS,
and answered on the site.

A media day was held on 20 August with representatives from magazines,
newspapers, television, NOAA, and North Carolina Sea Grant in
attendance. The guests had the opportunity to watch a submersible launch
and recovery, tour the ship, visit the ROV locker, eat lunch and talk
with the science party. The NOAA team on board assembled a CD-ROM of
highlight images. This CD-ROM was distributed at the end of the visit.

Art Howard of NAPRO Communications joined the trip on 20 August to
continue work on the High-Definition movie for the North Carolina State
Museum that he had begun during last year's Ocean Exploration mission.
During the 2003 mission he filmed during three submersible dives. He
also shot footage of some of the ship activities such as night lighting,
surveying and working up specimens. Additionally, he took a few hundred
still shots for use in the North Carolina State Museum publication, The
Naturalist. He shared his images with the science party and the NOAA
team.

An interesting benefit from having the connection with the North
Carolina State Museum was the identification of a nudibranch caught
early during the mission. Through email and the NOAA data pipe we were
able to send an image to the Museum. That image was then forwarded to a
researcher at the American Museum of Natural History in New York where
it was identified as the Blue Glaucus nudibranch.

Table 1. Dive Itinerary, 17-27 August 2003

+---------+---------+---------+---------+----------+----------------+
|         |         |         | SCI     |          |                |
|         |         |         | ENTIFIC |          |                |
|         |         |         | PE      |          |                |
|         |         |         | RSONNEL |          |                |
+---------+---------+---------+---------+----------+----------------+
| ### DAY | [DAT    | [DIVE   | [BO     | [ST      | [ARE           |
|         | E]{.und | #]{.und | W]{.und | ERN]{.un | A]{.underline} |
|         | erline} | erline} | erline} | derline} |                |
+---------+---------+---------+---------+----------+----------------+
| Su      | Aug. 17 | 3419    | Qu      | Sulak    | Stetson        |
|         |         |         | attrini |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Su      | Aug. 17 | 3420    | N       | Brooks   | Stetson        |
|         |         |         | izinski |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| M       | Aug. 18 | ROV     | na      | na       | Snowy Wreck    |
+---------+---------+---------+---------+----------+----------------+
| Tu      | Aug. 19 | 3421    | Qu      | Necaise  | MPA-2          |
|         |         |         | attrini |          |                |
+---------+---------+---------+---------+----------+----------------+
| Tu      | Aug. 19 | 3422    | Sulak   | Casazza  | MPA-2          |
+---------+---------+---------+---------+----------+----------------+
| W       | Aug. 20 | 3423    | Cassaza | Necaise  | MPA-1          |
+---------+---------+---------+---------+----------+----------------+
| W       | Aug. 20 | 3424    | Howard  | Q        | MPA-1          |
|         |         |         |         | uattrini |                |
+---------+---------+---------+---------+----------+----------------+
| Th      | Aug. 21 | 3425    | N       | Berg     | Southern       |
|         |         |         | izinski |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Th      | Aug. 21 | 3426    | Howard  | Brooks   | Southern       |
|         |         |         |         |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| F       | Aug. 22 | 3427    | Sulak   | Angell   | Southern       |
|         |         |         |         |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| F       | Aug. 22 | 3428    | Necaise | Randall  | Southern       |
|         |         |         |         |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Sa      | Aug. 23 | 3429    | Sulak   | Q        | Northern       |
|         |         |         |         | uattrini | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Sa      | Aug. 23 | 3430    | N       | Potter   | Northern       |
|         |         |         | izinski |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Su      | Aug. 24 | 3431    | Brooks  | Casazza  | Northern       |
|         |         |         |         |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| Su      | Aug. 24 | 3432    | Necaise | Berg     | Northern       |
|         |         |         |         |          | Lophelia       |
+---------+---------+---------+---------+----------+----------------+
| M       | Aug. 25 | 3433    | Howard  | Baird    | MPA-2          |
+---------+---------+---------+---------+----------+----------------+
| M       | Aug. 25 | 3434    | Sulak   | Randall  | MPA-2          |
+---------+---------+---------+---------+----------+----------------+
| Tu      | Aug. 26 | 3435    | Baird   | Casazza  | MPA-2          |
+---------+---------+---------+---------+----------+----------------+

Key:

MPA-1 = Site 1 Operations Area within Potential Boundaries of Marine
Protected Area

MPA-2 = Site 2 Operations Area within Potential Boundaries of Marine
Protected Area

ROV = Remote Operation Vehicle, used in lieu of submersible due to
weather conditions

Table 2. Summary Itinerary for Deck Operations:, 17-27 August 2003

  ------------------- -------------------- -------------------- ------------------- ---------------------------------------
  [DAY]{.underline}   [DATE]{.underline}   [SITE]{.underline}   [TIME               [OPERATIONS]{.underline}
                                                                SLOT]{.underline}   

  S                   Aug. 17              Stetson Lo.          05:00-24:00         Neuston

  M                   Aug. 18              Stetson Lo.          00:00-05:00         Fathometer survey

  M                   Aug. 18              Stetson Lo.          05:00-05:30         Tucker: midwater series

  M                   Aug. 18              Stetson Lo.          19:00-24:00         Tucker: midwater series

  Tu                  Aug. 19              So. Lo.              15:30-16:00         Neuston

  Tu                  Aug. 19              So. Lo.              20:00-24:00         Night light

  W                   Aug. 20              So. Lo.              00:00-02:00         Night light

  W                   Aug. 20              So. Lo.              02:30-04:30         Tucker: midwater series

  W                   Aug. 20              So. Lo.              05:30-06:00         Plankton

  W                   Aug. 20              So. Lo.              15:00-19:30         Dip net & angling

  W                   Aug. 20              So. Lo.              20:30-24:00         Plankton

  Th                  Aug. 21              MPA-2                00:00-04:00         Tucker: 10m, 20m, 50m series

  Th                  Aug. 21              transit              04:00-07:00         2-hr transit to Southern Lophelia +
                                                                                    survey site

  Th                  Aug. 21              So. Lo.              12:00-15:00         Neuston, small dual plankton net for
                                                                                    isotopes

  Th                  Aug. 21              So. Lo.              20:00-24:00         Tucker: 10m, 20m, 50m, 100m series

  F                   Aug. 22              So. Lo.              00:00-00:02         Fathometer survey, So. Lophelia-1
                                                                                    vicinity: reference position for start
                                                                                    = 33 34.3050 76 28.0220

                                                                                    

  F                   Aug. 22              So. Lo.              02:00-07:00         1-m plankton: 50m, 20m, 10m, 0m series,
                                                                                    30 min;

  F                   Aug. 22              So. Lo.              7:00                Fathometer survey target site 33
                                                                                    34.3050 76 28.0220

  F                   Aug. 22              So. Lo.              12:00-15:00         Neuston, small dual plankton net for
                                                                                    isotopes

  F                   Aug. 22              So. Lo.              20:00-24:00         Tucker: 400m, 200m, 100m - add down
                                                                                    weight if necessary

  Sa                  Aug. 23              transit              00:00-07:00         5-hr (48 n. mi. NE) transit to Northern
                                                                                    Lophelia + survey site

  Sa                  Aug. 23              No. Lo.              12:00-15:00         Neuston, small dual plankton net for
                                                                                    isotopes

  Sa                  Aug. 23              No. Lo.              20:00-24:00         Otter trawl in midwater as deeply as
                                                                                    possible

  Su                  Aug. 24              No. Lo.              00:00-03:00         Fathometer survey, start pos. TBA

  Su                  Aug. 24              No. Lo.              08:00-11:30         JSL-2 Dive 3431

  Su                  Aug. 24              No. Lo.              12:00-15:30         Plankton netting for isotope analyses

  Su                  Aug. 24              No. Lo.              16:00-20:00         JSL-2 Dive 3432 + digital camera
                                                                                    download

  Su                  Aug. 24              No. Lo.              20:00               80 n. mi. (9.5 hr) transit south to
                                                                                    MPA-2

  M                   Aug. 25              MPA-2                4:30                Arrive MPA-2 area

  M                   Aug. 25              MPA-2                08:00-12:00         JSL Dive 3433 + digital camera download

  M                   Aug. 25              MPA-2                12:00-14:30         Angling over MPA-2, JSL 3433 dive site

  M                   Aug. 25              MPA-2                15:00-19:30         JSL-2 Dive 3434 + digital camera
                                                                                    download

  M                   Aug. 25              MPA-2                20:00               Night light

  Tu                  Aug. 26              MPA-2                00:00-06:00         Night light

  Tu                  Aug. 26              MPA-2                08:00-10:30         JSL-2 Dive 3435
  ------------------- -------------------- -------------------- ------------------- ---------------------------------------

Key:

Stetson Lo. = Stetson Lophelia Exploratory Area

So. Lo. = Southern Lophelia Banks Site

No. Lo. = Northern Lophelia Banks Site

Tucker = 2m Tucker Trawl

Plankton = micron mesh, 1m plankton net

Dual Plankton = dual 0.333mm + 0.122mm plankton net for isotope samples

Neuston = 2x1m, mesh, surface neuston net

Otter Trawl = 3m, 0.25cm lined, semi-balloon trawl

Night Light = submersible light + nocturnal dip net sampling

Dip Net = daytime dip net sampling

Table 3: Types and file size of data

+----------------------------------+--------------------+-------------+
| [Data Collected]{.underline}     | [File              | [Total      |
|                                  | Type]{.underline}  | Size]{      |
|                                  |                    | .underline} |
+----------------------------------+--------------------+-------------+
| # S                              | Data (.dat)        | 265 MB      |
| easurface Water &Atmosphere Data |                    |             |
+----------------------------------+--------------------+-------------+
| Depth & Temperature for Net      | Seabird Plot39     | 877 KB      |
| Operations                       | (.asc)             |             |
+----------------------------------+--------------------+-------------+
| Acoustic Doppler Current         | Multiple types     | 3.5 GB      |
| Profiler                         |                    |             |
+----------------------------------+--------------------+-------------+
| Digital Voice Recordings         | Sony Memory (.MSV) | 1GB         |
+----------------------------------+--------------------+-------------+
| Fathometer Survey Screen Images  | Bitmaps (.bmp)     | 500 MB      |
+----------------------------------+--------------------+-------------+
| JSLII CTD Dive Data              | Text               | 4.5 MB      |
+----------------------------------+--------------------+-------------+
| High-Definition Video            | Hi-def video       | 10 hours    |
|                                  | format             |             |
+----------------------------------+--------------------+-------------+
| Digital Video (JSL II )          | Mini DV w/ DVD     | \~60 hours  |
|                                  | copy               |             |
+----------------------------------+--------------------+-------------+
| Digital Photographs (JSLII       | Jpegs              | 729 MB      |
| Digital Cam)                     |                    |             |
+----------------------------------+--------------------+-------------+
| Digital Photographs (Light       | Jpegs and Tiffs    | 1.97 GB     |
| table)                           |                    |             |
+----------------------------------+--------------------+-------------+
| JSLII Postion Data               | Text (.txt)        | 50 MB       |
+----------------------------------+--------------------+-------------+

![](media/image1.jpeg){width="7.877083333333333in"
height="6.018055555555556in"}

![](media/image2.jpeg){width="7.877083333333333in"
height="6.007638888888889in"}

![](media/image3.png){width="9.748611111111112in"
height="5.738194444444445in"}

![](media/image4.png){width="9.125196850393701in"
height="6.199212598425197in"}

Appendix I. Ocean Exploration, Life on the Edge Expedition, 16-27 August
2003. Surface-Deployed Sampling Station Data.

  ----------------------- -------------------- -------------------- -------------------- ------------------ ------------------ ------------------ -------------------
  [STATION]{.underline}   [DATE]{.underline}   [GEAR]{.underline}   [TYPE]{.underline}   [START             [START             [END               [END
                                                                                         LAT]{.underline}   LON]{.underline}   LAT]{.underline}   LONG]{.underline}

  sl03-001                17;Aug;03            neuston net          surface              3202.129           7738.841           3202.251           7737.290

  sl03-002                17;Aug;03            neuston net          surface              3202.297           7735.980           3202.262           7734.430

  sl03-003                17;Aug;03            neuston net          surface              3201.308           7738.627           3201.055           7737.040

  sl03-004                17;Aug;03            neuston net          surface              3221.037           7725.157           3221.049           7724.067

  sl03-005                17;Aug;03            neuston net          surface              3220.967           7723.340           3220.860           7725.544

  sl03-006                18;Aug;03            Rope                 surface              3326.661           7650.182           3326.661           7650.182

  sl03-007                18;Aug;03            tucker trawl         mid-water            3320.354           7706.207           3319.452           7706.332

  sl03-008                18;Aug;03            tucker trawl         mid-water            3315.306           7659.621           3314.614           7659.917

  sl03-009                18;Aug;03            tucker trawl         mid-water            3309.161           7653.595           3309.014           7653.574

  sl03-010                19;Aug;03            neuston net          surface              3330.405           7633.967           3329.426           7633.918

  sl03-011                19;Aug;03            neuston net          surface              3329.030           7633.882           3327.967           7633.648

  sl03-012                19;Aug;03            dip net (nite lite)  surface              3324.986           7705.489           3325.301           7705.746

  sl03-013                19;Aug;03            dip net (nite lite)  surface              3325.312           7705.755           3325.695           7706.049

  sl03-014                19;Aug;03            dip net (nite lite)  surface              3325.695           7706.049           3326.046           7706.307

  sl03-015                19;Aug;03            dip net (nite lite)  surface              3326.046           7706.307           3326.436           7706.561

  sl03-016                20;Aug;03            dip net (nite lite)  surface              3325.691           7650.176           3326.816           7648.769

  sl03-017                20;Aug;03            tucker trawl         mid-water            3326.392           7649.251           3325.663           7650.016

  sl03-018                20;Aug;03            tucker trawl         mid-water            3324.807           7651.068           3324.272           7651.310

  sl03-019                20;Aug;03            plankton net         surface              3323.526           7648.530           3323.397           7646.286

  sl03-020                20;Aug;03            dip net (H & L)      surface              3324.745           7705.026           3326.204           7704.293

  sl03-021                20;Aug;03            dip net              surface              3329.804           7656.506           3329.804           7656.506

  sl03-022                20;Aug;03            plankton net         mid-water            3330.495           7654.698           3330.546           7652.350

  sl03-023                20;Aug;03            plankton net         mid-water            3330.527           7649.730           3330.469           7647.227

  sl03-024                20;Aug;03            plankton net         mid-water            3330.452           7644.427           3330.505           7641.102

  sl03-025                20;Aug;03            plankton net         mid-water            3330.508           7639.483           3330.617           7635.935

  sl03-026                21;Aug;03            tucker trawl         mid-water            3330.458           7635.738           3330.254           7636.124

  sl03-027                21;Aug;03            tucker trawl         mid-water            3329.988           7636.364           3329.686           7636.473

  sl03-028                21;Aug;03            tucker trawl         mid-water            3332.874           7630.804           3332.509           7630.751

  sl03-029                21;Aug;03            tucker trawl         mid-water            3332.123           7630.935           3331.823           7631.118

  sl03-030                21;Aug;03            plankton net         surface              3336.688           7624.956           3337.939           7621.338

  sl03-031                21;Aug;03            plankton net         surface              3338.513           7619.657           3339.648           7616.059

  sl03-032                21;Aug;03            tucker trawl         mid-water            3337.600           7624.333           3337.268           7624.593

  sl03-033                21;Aug;03            tucker trawl         mid-water            3337.904           7623.629           3337.648           7623.887

  sl03-034                22;Aug;03            plankton net         surface              3334.543           7627.673           3335.045           7627.835

  sl03-035                22;Aug;03            plankton net         surface              3334.346           7627.579           3334.629           7627.340

  sl03-036                22;Aug;03            neuston net          surface              3334.305           7627.640           3334.527           7627.587

  sl03-037                22;Aug;03            neuston net          surface              3334.436           7627.666           3334.858           7626.441

  sl03-038                22;Aug;03            neuston net          surface              3334.384           7627.792           3334.420           7627.039

  sl03-039                22;Aug;03            dip net(sargassum)   surface              3334.299           7628.018           3334.383           7627.906

  sl03-040                22;Aug;03            plankton net         surface              3334.453           7627.443           3334.506           7627.279

  sl03-041                22;Aug;03            tucker trawl         mid-water            3333.924           7627.809           3333.575           7627.965

  sl03-042                22;Aug;03            tucker trawl         mid-water            3333.600           7627.544           3333.119           7627.496

  sl03-043                23;Aug;03            plankton net         surface              3411.516           7553.702           3411.793           7553.494

  sl03-044                23;Aug;03            otter trawl          mid-water            3420.543           7546.916           3420.204           7547.251

  sl03-045                23;Aug;03            otter trawl          mid-water            3419.046           7547.604           3418.485           7547.486

  sl03-046                23;Aug;03            dip net(nite lite)   surface              3419.191           7545.486           3420.352           7543.333

  sl03-047                24;Aug;03            dip net(nite lite)   surface              3420.352           7543.333           3421.437           7541.677

  sl03-048                24;Aug;03            dip net(nite lite)   surface              3421.437           7541.677           3421.437           7541.677

  sl03-049                24;Aug;03            dip net(nite lite)   surface              3422.590           7539.770           3423.390           7538.492

  sl03-050                24;Aug;03            dip net(nite lite)   surface              3423.409           7538.460           3424.548           7536.608

  sl03-051                24;Aug;03            plankton net         surface              3419.733           7546.841           3419.292           7547.253

  sl03-052                24;Aug;03            plankton net         surface              3419.377           7547.192           3419.341           7547.237

  sl03-053                24;Aug;03            plankton net         surface              3419.413           7547.239           3419.361           7547.277

  sl03-054                24;Aug;03            plankton net         surface              3419.404           7547.251           3419.329           7547.259

  sl03-055                24;Aug;03            plankton net         mid-water            3419.240           7547.170           3419.097           7547.186

  sl03-056                25;Aug;03            dip net(nite lite)   surface              3326.095           7702.957           3326.912           7702.770

  sl03-057                25;Aug;03            dip net(nite lite)   surface              3326.973           7702.751           3327.341           7702.633

  sl03-058                25;Aug;03            Hook & Line          surface              3324.996           7704.613           3324.996           7704.613

  sl03-059                25;Aug;03            dip net(nite lite)   surface              3325.944           7704.974           3326.224           7705.073

  sl03-060                25;Aug;03            dip net(nite lite)   surface              3326.231           7705.075           3326.518           7705.205

  sl03-061                25;Aug;03            dip net(nite lite)   surface              3326.524           7705.210           3326.771           7705.379

  sl03-021                25;Aug;03            dip net(nite lite)   surface              3326.779           7705.384           3326.983           7705.565

  sl03-063                25;Aug;03            dip net(nite lite)   surface              3326.983           7705.565           3327.195           7705.784

  sl03-064                25;Aug;03            dip net (H & L)      surface              3327.200           7705.788           3327.382           7706.003

  sl03-065                26;Aug;03            dip net(nite lite)   surface              3327.462           7706.084           3327.631           7706.272

  sl03-066                26;Aug;03            dip net(nite lite)   surface              3327.649           7706.290           3327.793           7706.437

  sl03-067                26;Aug;03            dip net(nite lite)   surface              3323.984           7704.208           3324.069           7704.412

  sl03-068                26;Aug;03            dip net(nite lite)   surface              3324.090           7704.487           3324.110           7704.626

  sl03-069                26;Aug;03            dip net(nite lite)   surface              3324.110           7704.639           3324.128           7704.792

  sl03-070                26;Aug;03            dip net(nite lite)   surface              3324.134           7705.023           3324.148           7705.164

  sl03-071                26;Aug;03            dip net(nite lite)   surface              3324.151           7705.177           3324.166           7705.268
  ----------------------- -------------------- -------------------- -------------------- ------------------ ------------------ ------------------ -------------------

Appendix II. Compilation of standard data entry forms employed by the
Co-PI team. These forms were provided in an accompanying hardcopy due to
a lack of scanner at sea to capture formats.

1\) Ship Station Log

2\) Sub Station Log Cover Sheet

3\) Sub Station Log Audiolog Transcription Sheet

4\) SeaBird Data Log for Tucker Trawls

5\) Stable Isotope Data Log

6\) JSL Sub Video Log

7\) Digital Photo Log

8\) Film Photo Log

9\) Sub Catch Log

10\) RV Surface Operations Catch Log