DIANA SAMMATARO

**Email: *dsammataro@tucson.ars.ag.gov ***

# USDA-ARS Carl Hayden Honey Bee Research Center

**2000 E. Allen Road, Tucson, AZ 85719-1596**

***Work Phone: 520 670 6380 ex 121; Fax 520 670 6493, Cell: 520 975
4122***

***http://gears.tucson.ars.ag.gov/home/sammataro/index.html***

\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*\*

**[EDUCATION]{.underline}**

**Ph.D.** **Entomology**, 1995. The Ohio State UniversitY, Dept.
Entomology, Columbus, OH, 43210. Dissertation: *Studies on the control,
behavior, and molecular markers of the tracheal mite (Acarapis woodi
(Rennie)) of honey bees* (Hymenoptera: Apidae).

**M.S. Urban Forestry,** 1977 University of Michigan, Ann Arbor, MI
48104.

**B. S.** **Landscape Architecture**, 1970 University of Michigan, Ann
Arbor.

**[HONORS and AWARDS]{.underline}**

-   1995 Eastern Apiculture Society Certificate of Excellence for
    *Outstanding Graduate Student in Apiculture.*

-   1995 The Ohio State University Dept. Entomology Service Award.

-   1999 Silver Medal Winner - Apimondia, Vancouver, BC, Canada.

-   1999 Gold Medal Winner - Apimondia, Vancouver, BC, Canada. *Honey
    Bee Pests, Predators and Diseases*. 3^rd^ Edition Chapter 8 --
    Insects: Dipteran Pests of Honey Bees.

-   2004 Dr. Sammataro first recipient of Hoopingarner Award for Best
    Scientific Presentation at the American Beekeeping Federation
    Convention, St. Augustine, FL.

-   2005 Acarology Research Award, for outstanding contributions;
    International Journal Acarology.

-   2006 Exceptional Service Award for outstanding Work in Apicultural
    Research, from the Apiary Inspectors of America.

**[Grants Awarded]{.underline}**

BARD-USDA grant, 1995-1998

Eastern Apiculture Society Research Foundation, 1994 & 1997

Harry S. Mesloh Scholarship Fund, June 1995

National Assn. Strawberry Growers, 1997

North Dakota Beekeepers Assn. 1997-1998

Ohio Fruit Growers Society, 1996 & 1997

Ohio Rural Rehabilitation Program, 1997

Ohio Vegetable Growers Assn. 1996 & 1997

OSU Service Award, Dept. Entomology, 1995

Tri-County (OH) Beekeepers Assn. 1997

Iowa State Beekeepers Assn. 1998

NM State Beekeepers Assn. 1999

Queen (Honey Bee) Breeders Assn. 1999

S.A.F.E. Technologies, 1999

PMAP (co-PI) 2000

CRADA with Cerexagri 2002-3.

California State Beekeepers Association, 1992, 1993, 1997-1999, 2003

National Honey Board, 2005

Almond Board of California, 1997, 2003, 2004, 2005

CRADA with PIMA Research and USDA (Dr. J. Hooper) 2003-6

> **[Organizations and Committees]{.underline}**

-   Entomological Society of America

-   Scientific Member of the Center for Insect Science, University of
    Arizona

-   American Assn. Professional Apiculturists

-   Acarology Development Foundation

-   Acarological Society of America, Chair: Student Awards Committee

-   Safety Committee, USDA-ARS Carl Hayden Bee Research Center

-   Eastern Apiculture Society, Life Member

##### [WORK EXPERIENCE]{.underline}

1\. **Research Entomologist, Carl Hayden Honey Bee Research Lab**. Dates
Employed: 03/2002 to present. Duties: Work on developing a novel
approach to managing bees and parasitic mites, including new mite
controls (using bee and mite volatiles and new natural products) and
research on pollination problems.(Supervisor: Gloria Hoffman).

2\. **Research Assistant, Dept. Entomology, Penn State University**, 501
ASI Bldg., University Park, PA 16802. Dates Employed: 06/1998 to
03/2002. Duties: Applied research on IPM controls of bee mites. Conduct
experiments, collect data, write results and give presentations. Worked
with an IPM program for managing honey bee mites. Developed easier
monitoring boards for varroa mites; photographed bee plants for web
page; looking to alternative pollinators for important commercial crops
and native plants; explored the effects sub-lethal pesticides may have
on these pollinators. (Supervisor\'s Name: N. Ostiguy. Phone: 814
863-2872.) For six months, worked part-time in the Plant Pathology
Department working with the Plum Pox Virus of Stone Fruits. Helped write
and publish ID guides, extension and public outreach information.

3\. **Ohio State University/ OARDC, 1680 Madison Ave, Wooster, OH**
44691. Dates Employed: 09/1995 to 04/1998. Duties: Worked as a post-doc
at the Bee Lab. I was working under the BARD grant (under Dr. Needham)
on the IPM bee mite program (Supervisor\'s Name: Dr. James Tew, 330 345
8336.)

4\. **Ohio State University, Dept Entomology, 103 B & Z Bldg., Columbus,
OH** 43210-1220. Dates 09/1991 to 06/1995. Duties: Teaching assistant
for biology, then a post doc. Developed a sugar/oil patty to control
tracheal mites. Dr. Glen Needham. Phone: 614 292 7609.

5\. Sales Manager, Bee Supplies. **A.I. Root Company, 623 W. Liberty
Street, Medina, OH** 44256. Dates Employed: 06/1988 to 09/1991. Duties:
Manage beekeeping equipment and supply sales, dealers and customer
service; write ads, bee literature and equipment instructions; maintain
company hives, give tours, and R&D for new equipment. (Supervisor\'s
Name: John Root. Phone: 1-800-BUYROOT.)

> **[Other Bee Related jobs]{.underline}**

**09/1981-06/1983** *Research Assistant.* USDA Honey Bee Research Lab.
Madison, WI. Assist in research of floral UV patterns for sunflowers;
SEM and TEM of flower and honey bee structures; field and work; teach
labs and classes, give demonstrations.

**1977--1980** *Peace Corps Volunteer.* Honey Bee Specialist;
Philippines. Write teaching handbook, conduct seminars, write articles;
field research on Varroa mites, teach entomology at local university,
assist in cottage industries.

**[job-related courses]{.underline}**

> 2005 *Summer Program for Acarology,* Introduction to Acarology, Ohio
> State Univ., (OSU) Columbus, OH
>
> 2005 *Presenting Data and Information,* Edward Tufte; Phoenix, AZ
>
> 2001 *Master Gardener's Course*, Penn State University, Univ. Park, PA
>
> 2000 *Forensic Entomology*, K.C. Kim, Penn State
>
> 1995 *Summer Program for Acarology,* Mites/ticks of medical and
> veterinary importance, (OSU)
>
> 1994 *Instrumental Insemination of Honey Bee Queens,* Susan Cobey,
> instructor OSU
>
> 1992 *Summer Program for Acarology,* Agricultural Mites, OSU
>
> **[Publications: Books]{.underline}**

Flottum, P.K. & **D. Sammataro.** 1988. *The New Starting Right with
Bees.* A.I. Root Co., Medina OH.

**Sammataro,** D. & A. Avitabile. 1998. *The Beekeeper's Handbook*. 3rd
ed. Cornell University Press, Ithaca: NY

**Sammataro, D.** 1997. *Diptera (Flies)*. R.A. Morse, ed. *Honey Bee
Pests, Predators and Diseases*, 3rd edition. pp 145-160. The A.I. Root
Co., Medina OH.

**Sammataro, D.** 1995. *Studies on the control, behavior and molecular
markers of the tracheal mite (*Acarapis woodi *\[Rennie\]) of honey bees
(Hymenoptera: Apidae)*. Ph.D. dissertation. The Ohio State University,
Columbus OH.

###### Videos and Web page

*VARROA MITES: Life Cycle, Detection and Control.* 1999. Penn State
University AV Dept. D. Sammataro, producer/writer. Second place winner
at 1999 Apimondia Conference, Vancouver, B.C.

TRACHEAL MITES 1994. *Dissecting mites using the tracheal pull method*
and 1995 *Host-seeking behavior of tracheal mites on honey bees*. D.
Sammataro, producer/writer. R. Smith, Vesta Video Productions, Toledo,
OH.

**ANIMAPS** Institute of Mathematical Geography, Sandra L. Arlinghaus,
William D. Drake, and John D. Nystuen (University of Michigan and
Community Systems Foundation) with data and other input from Audra Laug,
Kris S. Oswalt, Diana Sammataro; University of Michigan; Community
Systems Foundation; Carl Hayden Bee Research Center, (respectively).
Animated map of the spread of the varroa mite:
http://www-personal.umich.edu/\~sarhaus/solstice/sum01/varroa2001.html

> **[Scientific work]{.underline}**

Benoit, J. B., J. A. Yoder, **D. Sammataro** and L. W. Zettler. 2004.
Mycoflora and Fungal Vector Capacity of the Parasitic Mite, *Varroa
destructor* (Mesostigmata : Varroidae) in honey bee (Hymenoptera
:Apidae) colonies. International J. Acarology. 30(2): 103-106.

Needham, G.R., U. Gerson & **D. Sammataro**. 1999. Introduction. In:
*Mites of the Honey Bee*. Webster T.C., K.S. Delaplane, ed. Hamilton,
IL: Dadant & Sons.

Ostiguy, N., **D. Sammataro**. 1999. A Simplified Technique for Counting
Varroa Sticky Boards. *Apidologie*, 31: 707-16.

Robacker, D.C., P.K. Flottum, **D. Sammataro** and E.H. Erickson, Jr.
1983. Effects of climatic and edaphic factors on soybean flowers and on
the subsequent attractiveness of the plants to honey bees. *Field Crops
Res.* 6: 267-78.

**Sammataro D,** E.H. Erickson, Jr. & M.B. Garment. 1985. Ultrastructure
of the sunflower (*Helianthus*) nectary. *J. Apicultural Research*
24(3): 150-160.

**Sammataro D,** G. Degrandi-Hoffman, G.R. Needham and G. Wardell. 1998.
Some volatile plant oils as potential control agents for varroa mites
(Acari:Varroidae) in honey bee colonies (Hymenoptera: Apidae). *Amer.
Bee J.* 138: 681-685.

**Sammataro D**, G. Degrandi-Hoffman, N Ostiguy, G. Wardell and J.
Finley. 2004. Testing a combination of control strategies to manage
*Varroa destructor* (Acari: Varroidae) population levels in honey bee
(Hymenoptera: Apidae) colonies. International J. Acarology. 30:71-76.

**Sammataro D,** J. Finley & S. Camazine. 1999. Shipping conditions of
honey bee queens. *Amer. Bee J.* 139: 713-16.

**Sammataro D,** M.B. Garment & E.H. Erickson, Jr. 1985. Anatomical
features of the sunflower floret. *Helia* (FAO, Romania):25-31.

**Sammataro D**, N Ostiguy and M. Frazier. 2002. How to use a PSU/ IPM
Varroa board. *Amer. Bee J.* 142: 363-366*.*

**Sammataro D,** S. Cobey, B.H. Smith and G.R. Needham. 1994.
Controlling tracheal mites (Acari: Tarsonemidae) in honey bees
(Hymenoptera: Apidae) with vegetable oil. *J. Econ. Entomol.* 57(4):
910-916.

**Sammataro D,** U. Gerson and G.R. Needham. 2000. Parasitic Mites of
Honey Bees: Life History, Implications and Impact. *Ann. Rev. Entomol*.
45: 517-546. ParasiticMitesOfHoneyBees.pdf

**Sammataro D.** and G.R. Needham. 1996. Developing an integrated pest
management (IPM) scheme for managing parasitic bee mites. *Amer. Bee J.*
136: 440-443.

**Sammataro D**. and G.R. Needham. 1996. Host-seeking behaviour of
tracheal mites (Acari: Tarsonemidae) on honey bees (Hymenoptera:
Apidae). *Exp. Appl. Acarology,* 20: 121-136.

**Sammataro D.** and G.R. Needham. 1996. How oil affects the behavior of
tracheal mites. *Amer. Bee J.* 136: 511-514.

**Sammataro D.** 1996. Mechanisms of bee resistance/tolerance to varroa
mites. *Amer. Bee J.* 136: 567-68.

**Sammataro D.** 1996. Tracheal mites can be suppressed by oil patties.
*Amer. Bee J.* 136: 279-282.

**Sammataro D.** 1997. Report on parasitic honey bee mites and disease
associations. *Amer. Bee J.* 137: 301-302.

**Sammataro D.** 2004. *Tropilaelaps* infestation of honey bees
(*Troplilaelaps clareae, T. koenigerum*). *In* OIE Manual of Diagnostic
Tests and Vaccines for Terrestrial Animals, 5th ed. Office Internat.
Epizoot. Paris, France. Vol. 2: 992-995.

**Sammataro D.** 2006. Mites of the Honey Bee. *Bee Craft,* 88:14-19.

**Sammataro D**., P. Untalan, F. Guerrero and J. Finley. 2005. The
Resistance of Varroa Mites (Acari: Varroidae) to Acaricides and the
Presence of Esterase. *International J. Acarology*, 31:67-74.

**Sammataro, D**. and J. Finley. 2004. Observations of the ectoparasitic
bee mite *Varroa destructor* in honey bee (*Apis mellifera*) cells
infected with chalkbrood (*Ascosphaera apis). J. Apicultural Research,*
43 (1): 28-30.

Yoder, J. and **D. Sammataro**. 2003. Potential to control of *Varroa*
mites (Acari: Varroidae) using chemical ecology. *Internat. J.
Acarology.* 29: 139-143.

Yoder, J., **D. Sammataro**, J.A. Peterson, G.R. Needham and W.A. Bruce.
1999. Water requirements of adult females of the honey bee parasitic
mites, *Varroa jacobsoni*. (Acari: Varroidae) and implications for
control. *Internat. J. Acarology*. 25: 329-335.

**[[Manuscripts in Review]{.underline}]{.smallcaps}**

D. Sammataro and J. Finley. 2006. A Scientific Note on the Changes in
Development Times of Immature African and European Honey Bee Workers. In
review *J. Insect Science*.

G.M. Loper, D. Sammataro, J. Finley and J. Cole. 2006. Feral honey bees
in Southern Arizona, 10 years after Varroa infestation. For Am*. Bee J.*

D. Sammataro. 2006. An Easy Dissection Technique for Finding Tracheal
mites (Acari: Tarsonemidae) in Honey Bees (with Video). Internal Review.

***[Proceedings and Abstracts]{.underline}***

**Sammataro D.** J. Finley and F. D. Guerrero. 2004. Esterase activity
of varroa mites that are resistant to acaricides in honey bee colonies.
Proc. 2004 Annual Conf. Apiary Inspectors of Am. Jan. 6-11, San Antonio,
TX. p. 58-59.

Bell, D., S. Gloor, **D. Sammataro** and S. Camazine. 1999. Biochemical
mechanisms of fluvalinate resistance in varroa mites. *Amer. Bee J.*
138: 308-9.

Finley, J., **D. Sammataro** and S. Camazine. 1999. Queen shipment: the
missing link between producer and consumer. *Proc. Apimondia Congress
XXXVI*, Vancouver, B.C. p. 243.

Finley, J., **D. Sammataro** and S. Camazine. 1999. Queens in transit:
spying on the post office. *Am. B. J.* 138: 309.

Ostiguy, N., **D. Sammataro** and S. Camazine. 1999. How to count
*Varroa jacobsoni* without going blind: a sane approach. *Amer. Bee J.*
138: 313-14.

**Sammataro D,** J. Finley and S. Camazine. 1999. Multi-state testing of
some essential oils for varroa control. *Proc. Apimondia Congress
XXXVI*, Vancouver, B.C. p. 100.

**Sammataro D,** J. Finley and S. Camazine. 1999. New approaches to
varroa mite management. *Proc. Apimondia Congress XXXVI*, Vancouver,
B.C. p. 143.

**Sammataro D**, N Ostiguy, J. Finley, M. Frazier, G. Degrandi-Hoffman,
and G. Wardell. 2002. A novel IPM approach to managing *Varroa
jacobsoni* mite (Acari: Varroidae) levels in honey bee (Hymenoptera:
Apidae) colonies. *Proc. II International Conf. On Africanized Honey
Bees and Bee Mites*, Tucson AZ. A.I. Root Co. p. 217.

**Sammataro D,** N. Ostiguy and M. Frazier. 2001. IPM for Honey Bee
Mites. *Entomological Soc. America National Meeting, San Diego, CA.*

**Sammataro D,** P. Parker, and G.R. Needham. 1997. Using PCR-based
*RAPD*s (Random Amplified Polymorphic DNA) to determine differences in
tracheal mite *Acarapis woodi* (Rennie) (Acari: Tarsonemidae)
populations. *Proc. IX Intn\'l Congress of Acarology*, 1994; Columbus,
OH.

**Sammataro D,** P.K. Flottum and E.H. Erickson. 1984. Factors
contributing to honey bee preferences in sunflower varieties. *Proc.
Sunflower Res. Workshop*. Bismark, ND.

**Sammataro D,** S. Cobey, B.H. Smith and G.R. Needham. 1997.
Vegetable-shortening patties control tracheal mites (Acari:
Tarsonemidae) in honey bees (Hymenoptera: Apidae). *Proc. IX
International Congress of Acarology,* 1994; Columbus, OH.

**Sammataro D.** and F. D. Guerrero. 2003. The identification of
esterase activity in varroa mites resistant to miticides. Ento. Soc.
Annual Meeting, Cincinnati, OH.

Yoder, J. and **D. Sammataro**. 1999. Water balance studies of *Varroa
jacobsoni*. *Proc. Apimondia Congress XXXVI*, Vancouver, B.C. p. 105.

**[*Bee Culture* Magazine Articles]{.underline}**

> 1979 Sept. Vol. 114, pp. 458-9: *Beekeeping in the Philippines*.
>
> 1986 May pp. 251-253*: Landscaping for home and hive*; Aug. pp. 422*:
> Landscaping for home and hive, II*; Sept. pp. 461-2: *Collecting Honey
> Bee Stamps*; Nov. p. 584: *Early Winters: Antique Equipment.*
>
> 1987 Jan. Vol. 115 pp. 42: *Bee Flora: The Milkweeds*.
>
> 1988 March Vol. 116 pp. 139-141: *Apiphilately*; Dec. pp. 700-1,709,
> 722: *Res. Review*.
>
> 1989 Jan. Vol. 117 pp. 10-15, 54: *May the Forest Be With You; Wax
> Flowers* (20-21); Feb. pp. 108-11*: Package Primer* (w/ K. Flottum);
> March pp. 160-163: *Package Primer II*; April pp. 226-7: *Package
> Primer III*; May pp. 297, 303: *Raising Waxies*; July pp. 406-7*: Duct
> Tales*. (w/ K. Flottum); Aug. pp. 477-479: *Simply Wax*; Sept. pp.
> 532-3: *Making Molded Candles*.
>
> 1990 Jan. Vol. 118 pp. 20-6: *Deserts, Droughts and the Drying of the
> American West*; April pp. 220-22: *Ukrainian Easter; Tracking Tracheal
> Mites* (206-8); May pp. 284-6: *Long Live the Queen*; Aug. p. 493:
> *Stamps in the News*; Oct. pp. 596-9: *Making Craftwax and Foundation
> Candles*; Nov. pp. 663-5: *Honey Candy*.
>
> 1991 Jan. Vol. 119 pp. 32-38: *Erosion.*
>
> 1992 July Vol. 120 pp. 393, 396-400: *Conducting a honey bee emergency
> demonstration (now a video).*
>
> 1993 July Vol. 121 pp. 393-5: *Perfect rounds*.
>
> 1994 Jan. Vol. 122 pp. 30-39: *Races.* w/PK Flottum
>
> 1995 Feb. Vol. 123. pp. 80-81: *9th International Congress of
> Acarology: A honey bee mite round table.* w/ E. Sugden & K. Williams
>
> **Other Popular Articles**
>
> Sammataro, D. 2002. Honey bees are your swimming pool: not a good mix.
> August *Master Gardner's Newslette*r, Tucson AZ.
>
> Sammataro D, N Ostiguy, M. Frazier. 2002 *How to use a PSU/ IPM Varroa
> board*. American Bee Journal. 142:363-366

#### presentations

**2006**

*January.* National meetings: American Honey Producers, Houston, TX;
American Beekeeping Federation Louisville, KY.

*Feb:* NC State Beekeepers, Asheville NC

*March:* KS/MO beekeepers, Overland Park, KS; Michigan State Univ. ANR
week, E. Lansing MI; 24^th^ Annual Science and Math Conf. for 7-12^th^
grade women in S. Arizona, Un. of Az, Tucson.

*April:* Ulster Bee Keeper\'s Association, N. Ireland

*Nov:* S.E. New England Beekeepers, New Haven, CT.

**2005**

*January.* National meetings: American Honey Producers, Tucson, AZ,
American Beekeeping Federation and American Assoc. Professional
Apiculturists, Reno NV.

*July:* Heartland Apiculture Assoc., Edwardsville, IL July 7-9.

*August:* Apimondia (Dublin, Ireland); and OIE Conference.

**2004**

*January:* National meetings: American Honey Producers and American
Beekeeping Federation.

*April:* Mass. State Beekeepers; Rhode Island State Beekeepers

*June:* Master Gardeners, Tucson AZ

*July:* North Carolina State Beekeepers, Charlotte

*September:* First European Conference of Apidology, *and* European
Group for Integrated Varroa Control http://web.uniudd.it/eurbee/ Udine,
ITALY

*November*: Idaho Honey Industry Assoc., and Entomological Soc. of Am.
Salt Lake City, UT; Acarology Section

*December:* Colorado State Beekeepers, Denver

**2003**

*January.* National meetings in Baton Rouge, LA (American Honey
Producers) and Kansas City, (American Beekeeping Federation).

*February:* Alabama In-Service Training Workshop on Pollination Biology;
and AL Beekeepers Assoc. Symposium.

*March:* Kansas Honey Producers 100^th^ Anniversary Meeting, keynote
speaker

*August:* IL Beekeeping Short Course.

*October:* MO State Beekeepers meeting; and Entomol. Soc. of Am.
Meeting, Cincinnati OH. Sammataro, D., J. Finley, and F. D. Guerrero.
2003. Varroa mite (Mesostigmata: Varroidae) resistance to pesticides in
honey bee colonies (Hymenoptera: Apidae).

**2002**

May 11. Arivaca Library. How to handle Problem Bees

August 5-9. Eastern Apiculture Society, Ithaca, NY: Short course
instructor, workshop and conference presenter. Honey Plants, Mite ID,
IPM of Bee Mites.

Nov. 1-2. Oregon State Beekeepers Pest and Disease Workshop.

Dec. Entomology Society of America, National meetings 2002, Invited
Speaker at Acarology Conference.

**2001**

Feb 19. Penn. Assoc. for Sustainable Agriculture Penn State University.
*How to Manage Problems in Honey Bee Colonies.*

March 3.Tri-County Beekeepers Assoc. Annual Meeting, Wooster, OH

March 10. Western PA Beekeepers Assn., Beaver Co. PA

March 13. 29th Annual Apiary Inspectors Workshop, Beltsville, MD. All
talks on IPM.

August 6-9. Eastern Apiculture Society, Bourne, MA: Short course
instructor, conference presenter. IPM of Bee Mites.

August 31. Millbrook Marsh Nature Center. *Honey bees: Sweetness and
Mites*.

Sept. 16. Centre Co. Beekeepers. Demonstration.

Oct. 2. Northeast Agro Forestry/Carbon Conference, Binghamton, NY.
*Honey from Trees*

Oct. 12. Tennessee State Beekeepers Assoc. Nashville (workshop and
lecture).

Nov. 10. PA State Beekeepers Assoc. Lewisberg.

Nov. 18. *Three B's and a C* Bee Club banquet, Williamsburg, PA.

**2000**

NJ Apiary Inspectors of America, Beltsville, MD

March 11: Beaver Co. Beekeepers; March 23: Centre Co. Beekeepers (PA)

April 10-12: 2nd International Conference on African Bees and Bee Mites;
Tucson AZ: Moderator and present IPM 99 work

May 4: Philadelphia Soc. for Promoting Agriculture: *Honey Bees,
Sweetness and Mites*

May 13 Capital Area Bee Beekeeping Short Course

May 18 PA State Bee Inspectors

June 3: NE KS State Beekeepers Annual Meeting, Kansas City

June 17: Carroll Co. Beekeepers, OH

August 31. Millbrook Marsh Nature Center. *Important Pollinators*.

Oct 11: Centre Co. Beekeepers, PA and Millbrook Marsh Nature Center

Nov. 2 Oregon State Beekeepers Assn. Hood River, OR. IPM of bee mites.

Nov 13 PA State Beekeepers Assn. Lewisburg, PA. State Meeting

##### [OTHER PRESENTATIONS]{.underline}

###### *Apiary Inspectors of America*

1994 (all inspectors), 1997 (Ohio inspectors), 1998 & 2001 (PA
inspectors).

###### *Beekeeping Meetings, Lectures and Short Courses: State & County* 

**1999** *Apimondia 99, Vancouver B.C. International Bee Meeting:*
Presented two papers--- IPM of Bees and Water Balance of Bee Mites;
co-authored two other papers; Varroa video presentation (2nd place
winner).

Medieval Festival Bee Display on campus; Penn State Bee Short Course,
State meetings in: GA, NY, VT, TN, PA, Western CT, York Co. PA

Nov. 19-20: USDA-ARS meeting for bee researchers and stakeholders

**1998**: MD, SC, OH, MI, CA, CT, NM, NY, PA, VT and GA State Beekeepers

**1997**: Feb. Ohio Fruit Growers Assn.; March: Michigan State Un. ANR
Week; Tri-County BK; Scarlet-Oaks Bee Meeting; April: OH Apiary
Inspectors; OH Pickle Growers; June: OH Teachers Assn.; Georgia State
BK; MSU Mite Symposium; Aug: Western Apiculture Soc., Tucson; (Oct) MA
state BK & Iowa BK; Nov: PA State BK; Dec: Almond Board of California

**1996**: Feb.10: Archbold Biological Sta., FL for NY Empire State BK;
Feb.12: Ohio Fruit Growers Assn.; Mar. 10: Michigan State Un. ANR Week;
June 7: Ohio State BK (OSBA) July 19-21: BiOhio OSU, Wooster, OH; July
27: Ohio State BK (OSBA); July 29-31: Eastern Apiculture Society,
Harrisonburg, VA.; Sept.19: Farm Science Review, Ohio State University;
Oct. 11-13: North Dakota State BK; Oct. 18-20: Michiana BK; Oct. 25-27:
Indiana State BK; Nov. 1-3: Arkansas State BK; Nov. 8-10: Empire (New
York) State BK; Nov. 11-13: California State BK; Dec. 8: Ent. Soc.
America, Louisville, KY.

**1991 & 1990, 1994**, Urban Geography classes Dr. J. Nystuen, Un.
Michigan, Ann Arbor: *Importance of Honey Bees in Ag Food Systems*.

**Eastern Apiculture Society 1989-1999 & 2001-02**. Short Course
instructor, workshop and conference presenter.

**Entomology Society of America, National meetings 1992 & 1993**,
Organizer of Informal Symposium on Bee Mites. 1993, Invited Speaker at
Formal Acarology Conference; 1994, Organizer of Informal Symposium.

**Ohio State Fair**, 1994 Judge for 4-H show: *Exploring our Insect
World*

**Insect Fair**, Penn State University 1998 -2001 Helped set up and
organize Honey Bee Display; attendance 5,000

**BIO**

Diana Sammataro, co-author of the *Beekeeper's Handbook*, began keeping
bees in 1972 in Litchfield CT, setting up a package colony in her
maternal grandfather's old bee hive equipment. From then on, she decided
that her B.S. in Landscape Architecture (Un. of Michigan, Ann Arbor),
would not be a career, but that pollination ecology would. After a year
of independent studies with Dr. Bert Martin at the Mich. State Un. Bee
Lab (East Lansing, MI) she earned an M.S. in Urban Forestry (Un. of
Michigan, Ann Arbor). In 1978 she joined Peace Corps and taught
Beekeeping in the Philippines for 3 years. On returning, she worked at
the now closed USDA Bee Lab in Madison, WI under Dr. Eric Erickson,
studying the effects of plant breeding and flower attraction of bees in
sunflower lines. When Dr. Erickson moved to be head of the Tucson Bee
Lab, she eventually went to work at the A.I. Root Company, as Bee Supply
Sales Manager in Medina OH. In 1991 she was accepted at the Rothenbuhler
Honey Bee Lab at OH State University (Columbus, OH) to study for a Ph.D.
with Dr. Brian Smith and Dr. Glen Needham. The title of her dissertation
was: *Studies on the control, behavior, and molecular markers of the
tracheal mite (*Acarapis woodi *(Rennie)) of honey bees* (Hymenoptera:
Apidae).

In 1995, she worked as a post-doctoral assistant at the Ohio State Un.
Ag. Research Center in Wooster OH, with Dr. James Tew and in 1998 at the
Penn State University Bee lab (State College, PA, with Maryann Frazier.
In early 2002, she was invited to join the USDA-ARS Carl Hayden Honey
Bee Research Center in Tucson AZ. Her current position is a Research
Entomologist with Dr. Gloria DeGrandi-Hoffman and staff. Her work at the
lab includes developing a novel approach to managing parasitic mites of
bees using new mite controls (bee and mite chemical compounds and
natural products) and research on pollination problems.

#######  ABSTRACT of Dissertation

Title: *Studies on the control, behavior, and molecular markers of the
tracheal mite (Acarapis woodi (Rennie)) of honey bees* (Hymenoptera:
Apidae).

**Abstract**: The endoparasitic mite, *Acarapis woodi* (Rennie), a pest
of honey bees *Apis mellifera* L., infests most bee colonies in the
United States and has been responsible for 60% of colony losses. Under
field conditions, patties made from solid vegetable shortening and white
sugar, with or without the addition of an antibiotic, depressed mite
populations when applied continuously to bees. Treatment was significant
(Site 1, F~2,165~=14.95; P\<0.001; Site 2, F~2,96~=5.541; P\<0.001).

To understand why shortening/sugar patties gave bees some protection,
mite behavior was videotaped on callow bees (\<4 days old), dead bees
and bees exposed to an oil patty. Two behaviors were observed and more
closely studied. "Habitat-seeking" behavior, when mites seek out a new
oviposition site, was disrupted on both dead and oil-treated bees.
"Questing" behavior, associated with mite transfer between hosts,
increased on dead and oily bees. Both questing (F~2,66~ =7.88; P\<0.001)
and habitat-seeking (F~2,66~=21.28, P\<0.001) behaviors were
significantly different between all three treatments. Oil-treated bees
gained protection from habitat-seeking mites because the normal behavior
of the mites is interrupted. Questing behavior increased significantly
on dead and oily bees, thus exposing the mites for a longer time and
increasing the chances of desiccation.

In the decade since its introduction here, the lethal effects of this
mite seem to diminish. To determine if this was a change in the
lethality of mite populations, infested bees from several states were
collected and the mites dissected. RAPDs was used to track possible
shifts in genetic markers. Problems of reaction protocols, contamination
and clean negative controls were mostly solved by using HPLC water that
was not treated to UV light. PCR parameters where annealing temperatures
ranged from 38-45C also produced good results. However, because of
extremely low mite DNA concentrations, the results were inconclusive. By
testing a dilution series using one bee, it was found that as the
concentration of DNA diminished to 0.038ng/ul, more bands in the gel
appeared. RAPDs was not a good method of choice; this question still
needs to be explored as future techniques are refined.