[Questions]{.underline}

[Testing for DON (Vomitoxin)]{.underline}

**A**. **[General Questions]{.underline}**

1\. DON testing service is provided as official criteria for wheat,
barley, oats, and corn.

> \(a\) True (b) False

2\. DON, also referred to as is a naturally occurring mycotoxin produced
by several species of Fusarium.

\(a\) aflatoxin (c) fumonisin

\(b\) vomitoxin (d) zearalenone

3\. DON testing service is provided as a factor under the authority of
the United States Grain Standards Act (USGSA).

\(a\) official criteria (c) optional

\(b\) grade determining (d) special

4.  DON testing is available as a quantitative service only.

> \(a\) True (b) False

5.  DON test results exceeding 5.0 ppm exceed Food and Drug
    Administration (FDA) defect action limits and must be reported to
    the local FDA office.

> \(a\) True (b) False

6\. DON test results that are above the test kit\'s established
conformance limits are reported as exceeding the conformance limit
unless a supplemental analysis is performed.

\(a\) True (b) False

7\. To clean labware (i.e., glassware, funnels, beakers, etc.) used in
the DON testing process, prepare a solution consisting of .

\(a\) Bleach/water (c) Methanol/water

\(b\) Dishwashing liquid/water (d) Distilled water

8.  Applicants requesting DON testing must specify whether qualitative
    or quantitative testing service is desired.

> \(a\) True (b) False

9.  Unit trains and shiplots are analyzed on a sublot basis for wheat
    and barley and a composite basis for other grains.

> \(a\) True (b) False

10\. A review inspection for DON must be performed on the basis of file
samples.

\(a\) True (b) False

11\. How many field review inspections are permitted for shiplot, unit
train, or lash barge material portions when testing is performed on a
sublot basis?

> \(a\) None (c) Two
>
> \(b\) One (d) Four

12. Board Appeal DON testing is performed on the basis of

> \(a\) A new sample (c) A new sample or the file sample
>
> \(b\) The applicant\'s sample (d) The file sample

13\. A sample of approximately grams, with dockage and stones removed,
is required for the DON testing and file sample.

\(a\) 100 (c) 250

\(b\) 200 (d) 500

14\. How many grams must be maintained for the DON file portion?

> \(a\) approximately 25 (c) approximately 100
>
> \(b\) approximately 75 (d) approximately 200

15. When quantitative test results indicate that DON is present at a
    level of 0.5 ppm or less, certify the results as:

```{=html}
<!-- -->
```
(a) \"DON not detected.\"

(b) \"Don equal to or less than 2 ppm.\"

(c) \"DON 0.5 ppm estimated.\"

(d) \"DON less than or equal to 0.5 ppm.\"

```{=html}
<!-- -->
```
16. Quantitative test results between 0.6 ppm and the conformance limit
    are certified to the nearest whole ppm. Upon request of the
    applicant, results may be certified to the nearest tenth ppm.

> \(a\) True (b) False

17. Upon request of the applicant, DON results may be reported as parts
    per billion (ppb) instead of ppm. To convert ppm to ppb, multiply
    the ppm result by:

> \(a\) 10 (c) 1000
>
> \(b\) 100 (d) 10000

**B.** **[Neogen AgriScreen Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

(a) 50 grams of ground sample be mixed with 100 ml of 55% methanol/water

> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 250 ml of
> distilled/deionized water
>
> and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml of
> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. Test kits should be refrigerated at temperatures between 30°F and
40°F.

> \(a\) True (b) False

3\. After shaking the sample for 3 minutes, how long should the sample
settle before the extract is filtered?

> \(a\) 30 seconds (c) 90 seconds
>
> \(b\) 60 seconds (d) 120 seconds

4\. Allow the rehydrated conjugate solution to set for hour(s) prior to
use.

\(a\) Two (c) Four

\(b\) One (d) Three

5\. Once the conjugate is rehydrated it must be used within 3 weeks.

\(a\) True (b) False

6\. To perform testing, remove red-marked mixing well strip and break
off needed number of wells. One well for each sample and well(s) for
control(s).

\(a\) one (c) three

\(b\) two (d) five

7\. There are two separate incubation periods (referred to as waiting
periods in the instruction) in the testing process. What is the duration
of each waiting period?

\(a\) 1 minute (c) 5 minutes

\(b\) 2 minutes (d) 10 minutes

8\. To rinse the anti-body coated wells use a wash bottle filled with
distilled/deionized water and shake out. Repeat this step time(s).

\(a\) two (c) four

\(b\) three (d) five

9\. If a Microwell reader is used to read the results it must warm up
for a minimum of 30 minutes before using.

\(a\) True (b) False

10. Test results are reported on the pan ticket and inspection log as
    being equal to or less than a threshold (e.g., 1 ppm) or as
    exceeding a threshold.

> \(a\) True (b) False

C.  **[Neogen Veratox Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

> \(a\) 50 grams of ground sample be mixed with 100 ml of 55%
> methanol/water
>
> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 250 ml of
> distilled/deionized water
>
> and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml of
> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. Test kits should be refrigerated at temperatures between 30°F and
40°F.

> \(a\) True (b) False

3\. After shaking the sample for 3 minutes, how long should the sample
settle before the extract is filtered?

> \(a\) 30 seconds (c) 90 seconds
>
> \(b\) 60 seconds (d) 120 seconds

4\. Allow the rehydrated conjugate solution to set for hour(s) prior to
use.

\(a\) Two (c) Four

\(b\) One (d) Three

5\. Once the conjugate is rehydrated it must be used within 3 weeks.

\(a\) True (b) False

6\. To perform qualitative testing, remove red-marked mixing well strip
and break off the needed number of wells. Use one well for each sample
and well(s) for control(s).

\(a\) one (c) three

\(b\) two (d) five

7\. There are two separate incubation periods (referred to as waiting
periods in the instruction) in the testing process. What is the duration
of each waiting period?

\(a\) 1 minute (c) 5 minutes

\(b\) 2 minutes (d) 10 minutes

8\. To rinse the anti-body coated wells, use a wash bottle filled with
distilled/deionized water and shake out. Repeat this step time(s).

\(a\) one (c) three

\(b\) two (d) five

9\. If a Microwell Model 301 reader is used to read the results it must
warm up for a minimum of 30 minutes before using.

\(a\) True (b) False

10\. For qualitative testing, results are reported on the pan ticket and
inspection log as being equal to or less than a threshold (e.g., 1 ppm)
or as exceeding a threshold.

> \(a\) True (b) False

11\. To perform quantitative testing, remove red-marked mixing well
strip and break off the needed number of wells. Use one well for each
sample and well(s) for controls.

\(a\) one (c) three

\(b\) two (d) five

**D. [Romer DON Fluoroquant Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

> \(a\) 50 grams of ground sample be mixed with 100 ml of 55%
> methanol/water
>
> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 200 ml of
> acetonitrile/water and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml of
> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. MycoSep columns must be refrigerated at temperatures between 36°F
and 40°F.

> \(a\) True (b) False

3\. The extraction solution is made by adding acetonitrile to distilled
or deionized water. What is the ratio of acetonitrile to
distilled/deionized water in the solution?

(a) 70% acetonirile to 30 % distilled/deionized water

(b) 70% distilled/deionized water to 30% actonitrile

> \(c\) 84% acetonirile to 16 % distilled/deionized water
>
> \(d\) 84% distilled/deionized water to 16% actonitrile

4\. Calibration and control solutions must be capped tightly and stored
in the refrigerator.

\(a\) True (b) False

5\. Evaporate each sample, calibrator, and control to dryness using a
vacuum manifold and dry bath set at [.]{.underline}

\(a\) 100°F (c) 70°F

\(b\) 100°C (d) 70°C

6.  During the derivatization process sample tubes are heated in a 50°C
    bath for 5 minutes, then cooled to room temperature before inserting
    into the fluorometer.

> \(a\) True (b) False

7.  What is the required warm-up period for the fluorometer?

> \(a\) 15 minutes (c) One hour
>
> \(b\) 30 minutes (d) No warm-up is necessary

8\. After the cuvette containing the sample portion is inserted into the
sample well of the fluorometer, the DON concentration will appear on the
fluorometer display after the appropriate delay.

\(a\) 2 second (c) 2 minute

\(b\) 10 second (d) 1 minute

9\. After Reagents A and B have been added to all sample tubes,
calibrators, and control, the tubes are capped and mixed with a vortex
for seconds.

\(a\) 5 (c) 15

\(b\) 10 (d) 30

10\. Calibrator standard and control standard bottles must be inverted
three times to mix the solutions.

> \(a\) True (b) False

**E. [Romer AccuTox Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

> \(a\) 50 grams of ground sample be mixed with 100 ml of 55%
> methanol/water
>
> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 200 ml of
> acetonitrile/water and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed into a plastic or glass
> container with 250 ml of distilled/deionized water and mixed by
> shaking (by hand or mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. Reagents, antibody coated tubes, and sample extracts must be at room
temperature prior to running a test.

> \(a\) True (b) False

3\. After enzyme conjugate has been added to the first tube, start a
timer set to the required incubation period of minutes.

\(a\) five (c) fifteen

\(b\) ten (d) twenty

4\. After the first incubation period the tubes must be dumped and
washed. How many times (at a minimum) must the tubes be washed?

\(a\) Two (c) Four

\(b\) Three (d) Five

5\. After the substrate has been added into the tubes the solutions
should all turn purple in color.

\(a\) True (b) False

6\. After adding the stop solution into the tubes the solutions should
turn in color.

\(a\) green (c) blue

\(b\) yellow (d) blue-green

7\. What is the name of the instrument used to read the absorbance
levels of the calibrator, control, and samples?

> \(a\) Fluorometer (c) GAC 2100
>
> \(b\) Microwell Reader (d) Spectrophotometer

8.  The reagents supplied with the test kits can be used until the
    expiration date on the kit label when stored refrigerated at
    temperatures between 36°F and 46°F.

> \(a\) True (b) False

9.  The enzyme conjugate must be pipetted directly into the bottom of
    the antibody coated tubes without touching the sidewalls.

> \(a\) True (b) False

10\. The spectrophotometer must be set at nm for calibration purposes.

\(a\) 450 (c) 500

\(b\) 0 (d) 300

**F. [Neogen Veratox 5/5 DON Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

> \(a\) 50 grams of ground sample be mixed with 100 ml of 55%
> methanol/water
>
> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 250 ml of
> distilled/deionized water
>
> and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml of
> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. Test kits should be refrigerated at temperatures between 30°F and
40°F.

> \(a\) True (b) False

3\. After shaking the sample for 3 minutes, how long should the sample
settle before the extract is filtered?

> \(a\) 30 seconds (c) 90 seconds
>
> \(b\) 60 seconds (d) 120 seconds

4\. Allow the rehydrated conjugate solution to reach room temperature
prior to running the test - approximately hour(s).

\(a\) Two (c) Four

\(b\) One (d) Three

5\. If a diluted sample extract is being analyzed, the Stat-Fax
microwell reader value for the extract must be modified to adjust for
the dilution of the extract.

\(a\) True (b) False

6\. To perform testing, remove one red-marked mixing well(s) for each
sample to be tested and red-marked well(s) for control(s).

\(a\) Two (c) Four

\(b\) Three (d) Five

7\. There are two separate incubation periods (referred to as waiting
periods in the instruction) in the testing process. What is the duration
of each waiting period?

\(a\) 1 minute (c) 5 minutes

\(b\) 2 minutes (d) 10 minutes

8\. To rinse the anti-body coated wells, use a wash bottle filled with
distilled/deionized water and shake out. Repeat this step time(s).

\(a\) One (c) Five

\(b\) Two (d) Ten

9\. To perform testing for wheat, oats, barley, malted barley and corn
at the 5 ppm conformance limit, the sample extract must be diluted with
distilled or deionized water.

\(a\) True (b) False

10\. The Veratox 5/5 DON test kit allows for an optional procedure for
testing at the 2.5 ppb conformance limit.

\(a\) True (b) False

11\. When test results indicate that DON is present at a level of 0.5
ppm or less, certify the results as:

\(a\) less than 0.5 ppm (c) 0.5 ppb or less

\(b\) 1 ppm (d) equal to or less than 0.5 ppm

**G. [Diagnostix EZ-Quant DON Plate Test - Specific
Questions]{.underline}**

1\. The EZ-Quant 0.5 PPM DON Plate Kit is approved for testing barley,
malted barley, and corn from 0.5 - 2.5 ppm

\(a\) True (b) False

2\. In the extraction process place 50 grams of ground sample into a
clean plastic bag and add of distilled or deionized water and seal/close
the bag securely to prevent spillage.

\(a\) 50 ml (c) 250 ml

\(b\) 100 ml (d) 1000 ml

3\. Reagents, antibody-coated wells, and mixing wells must reach room
temperature prior to running the test.

> \(a\) True (b) False

4\. What is the maximum number of test samples that can be run at one
time?

> \(a\) six (c) eighteen
>
> \(b\) twelve (d) nineteen

5\. To rinse the test wells, use a wash bottle filled with the wash
solution and fill each well to overflowing then dump the contents and
shake out any residue solution. Repeat this step

time(s).

\(a\) two (c) four

\(b\) three (d) five

6\. The extraction procedure requires that the ground sample and
extraction solvent (distilled/deionized water) must be mixed by shaking
vigorously for minutes.

\(a\) two (c) four

\(b\) three (d) five

7\. After shaking the ground sample portion and extraction solvent in
the extraction process, allow the extract to sit for minute(s) for the
slurry to settle.

\(a\) two - three (c) one - two

\(b\) one (d) five

8\. What instrument is approved for reading test results with the
Diagnostix EZ-Quant test kit?

\(a\) EL-301 microwell reader (c) Stat-Fax microwell reader

\(b\) RL-100 Fluorometer (d) Hach Spectrophotometer

9\. On the day the kit will be used it is acceptable to store the kit at
ambient room temperature (62°F to 82°F)

\(a\) True (b) False

10\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) fifteen

\(b\) ten (d) twenty

11\. Test kits should be refrigerated at temperatures between 36°F and
46°F.

> \(a\) True (b) False

12\. There are two separate incubation periods in the testing process.
The first incubation period is for ten minutes. What is the duration of
the second incubation period?

\(a\) one minute (c) five minutes

\(b\) two minutes (d) ten minutes

**H. [r-Biopharm Ridascreen Fast DON Test - Specific
Questions]{.underline}**

1\. In the extraction process place 50 grams of ground sample into a
suitable container (e.g., plastic bag) and add of distilled or deionized
water and seal/close the container securely to prevent spillage.

\(a\) 50 ml (c) 250 ml

\(b\) 100 ml (d) 1000 ml

2\. After shaking the ground sample portion and extraction solvent in
the extraction process, allow the extract to sit for minute(s) for the
slurry to settle.

\(a\) two - three (c) one - two

\(b\) one (d) five

3\. The filtered extract must be diluted to the ratio of one part sample
extract to three parts of

water.

\(a\) tap (c) deionized

\(b\) distilled (d) distilled or deionized

4\. When stored properly (at 39°F) the washing buffer solution has a
shelf life of

.

\(a\) one day (c) one week

\(b\) two days (d) four weeks

5\. What is the maximum number of samples that can be tested per set of
control standards?

> \(a\) six (c) eighteen
>
> \(b\) twelve (d) nineteen

6\. The extraction procedure requires that the ground sample and
extraction solvent (distilled/deionized water) must be mixed by shaking
vigorously for minutes.

\(a\) two (c) four

\(b\) [three]{.underline} (d) five

7\. There are two separate incubation periods in the testing process.
The first incubation period is for five minutes ([+]{.underline} 1
minute) at room temperature. What is the duration of the second
incubation period?

\(a\) one minute (c) five minutes ([+]{.underline} 1 minute)

\(b\) two minutes (d) three minutes (+ 0.5 minutes)

8\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) fifteen

\(b\) ten (d) twenty

9\. What instrument is approved for reading test results with the
r-Biopharm Ridascreen Fast DON test kit?

\(a\) EL-301 microwell reader (c) Stat-Fax microwell reader

\(b\) RL-100 Fluorometer (d) Hach Spectrophotometer

10. If test results are above the test method\'s conformance limit, test
    results are reported as exceeding the limit unless the applicant
    requests a supplemental analysis.

> \(a\) True (b) False

11\. Test kits should be refrigerated at temperatures between .

\(a\) 36°F and 46°F (c) 36°F and 66°F.

\(b\) 30°F and 40°F (d) 46°F and 56°F.

12\. To rinse the test wells, use a wash bottle and fill each well with
washing buffer solution then empty the wells to remove all remaining
liquid. Repeat this step times.

\(a\) two (c) four

\(b\) three (d) five

**I. [Strategic Diagnostics Myco DON Test - Specific
Questions]{.underline}**

1\. In the extraction process place 50 grams of ground sample into a
clean blender (e.g., plastic bag) and add of distilled or deionized
water and seal/close the container securely to prevent spillage.

\(a\) 50 ml (c) 250 ml

\(b\) 100 ml (d) 1000 ml

2\. After shaking the ground sample portion and extraction solvent in
the extraction process, allow the extract to sit for minute(s) for the
slurry to settle.

\(a\) two - three (c) one - two

\(b\) one (d) five

3\. There are two separate incubation periods in the testing process.
The first incubation period is for five minutes. What is the duration of
the second incubation period?

\(a\) one minute (c) five minutes

\(b\) two minutes (d) three minutes

4\. To rinse the test wells, use a wash bottle filled with the wash
solution and vigorously wash each well by overfilling. Repeat the
vigorous wash for a total of washes.

\(a\) two (c) four

\(b\) three (d) five

5\. What instrument is approved for reading test results with the Myco
DON Test kit?

\(a\) EL-301 microwell reader (c) Stat-Fax microwell reader

\(b\) Hyperion Microreader 3 (d) Hach Spectrophotometer

6\. If test results are above the test method\'s conformance limit, test
results are reported as exceeding the limit unless the applicant
requests a supplemental analysis.

> \(a\) True (b) False

7\. Test kits should be refrigerated at temperatures between .

\(a\) 36°F and 46°F (c) 36°F and 66°F.

\(b\) 30°F and 40°F (d) 46°F and 56°F.

8\. The extraction procedure requires that the ground sample and
extraction solvent (distilled/deionized water) must be blended on high
speed for minutes.

\(a\) two (c) four

\(c\) three (d) five

9\. To perform testing on a single sample, remove one red-marked mixing
well(s) for the sample to be tested and red-marked well(s) for
control(s).

\(a\) five (c) three

\(b\) four (d) two

10\. Test results should be read within minutes of completion of the
test.

\(a\) five (c) twenty

\(b\) ten (d) thirty

11\. The conformance limit for the Myco DON Test is ppm.

\(a\) 1 (c) 3

\(b\) 2 (d) 5

12\. To prepare the Wash Solution, transfer the contents of the Wash
Concentrate vial to a 500 ml plastic squeeze bottle and add ml of
distilled/deionized water.

\(a\) 500 (c) 400

\(b\) 475 (d) 200

**J.** **[Vicam DON FQ Test - Specific Questions]{.underline}**

1\. Sample extraction procedures require that:

> \(a\) 50 grams of ground sample be mixed with 100 ml of 55%
> methanol/water
>
> solution and blended on high speed for one minute.
>
> \(b\) 50 grams of ground sample be mixed with 200 ml of
> acetonitrile/water and blended on high speed for three minutes.
>
> \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml of
> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3 minutes.
>
> \(d\) None of the above.

2\. DON FQ columns must be refrigerated at temperatures between 36°F and
40°F.

> \(a\) True (b) False

3\. The extraction solution is made by adding acetonitrile to distilled
or deionized water. What is the ratio of acetonitrile to
distilled/deionized water in the solution?

(c) 70% acetonirile to 30 % distilled/deionized water

(d) 70% distilled/deionized water to 30% actonitrile

> \(c\) 84% acetonirile to 16 % distilled/deionized water
>
> \(d\) 84% distilled/deionized water to 16% actonitrile

4\. Calibration and control solutions must be capped tightly and stored
in the refrigerator.

\(a\) True (b) False

5\. Evaporate each sample, calibrator, and control to dryness using a
vacuum manifold and dry bath set at [.]{.underline}

\(a\) 100°F (c) 70°F

\(b\) 100°C (d) 70°C

6\. During the derivatization process sample tubes are heated in a 50°C
bath for 5 minutes, then cooled to room temperature before inserting
into the fluorometer.

> \(a\) True (b) False

7\. What is the required warm-up period for the fluorometer?

> \(a\) 15 minutes (c) One hour
>
> \(b\) 30 minutes (d) No warm-up is necessary

8\. After the cuvette containing the sample portion is inserted into the
sample well of the fluorometer, the DON concentration will appear on the
fluorometer display after the appropriate delay.

\(a\) 2 second (c) 2 minute

\(b\) 10 second (d) 1 minute

9\. After Reagents A and B have been added to all sample tubes,
calibrators, and control, the tubes are capped and mixed with a vortex
for seconds.

\(a\) 5 (c) 15

\(b\) 10 (d) 30

10\. Calibrator standard and control standard bottles must be inverted
three times to mix the solutions.

> \(a\) True (b) False

K.  **[Rosa DON P/N Test - Specific Questions]{.underline}**

1\. The ROSA® P/N test kit uses a lateral flow strip procedure that
provides qualitative results.

\(a\) True (b) False

2\. Test strips should be at room temperature (64 º - 86 ºF) prior to
use.

\(a\) True (b) False

3\. The extraction solvent used for the ROSA® P/N test is made up by
using the ratio of 70 parts of methanol to 30 parts of
\_\_\_\_\_\_\_\_\_ water.

> \(a\) distilled (c) distilled or deionized
>
> \(b\) deionized (d) tap

4\. What is the size of ground portion that is used for extraction?

> \(a\) 50 grams (c) 100 grams
>
> \(b\) 250 grams (d) one pound

5\. In the extraction process, \_\_\_\_\_\_\_ ml of the extraction
solvent is added to the ground portion and shaken 60 seconds and allowed
to settle for one minute.

> \(a\) 250 (c) 50
>
> \(b\) 100 (d) 150

6\. For screening level 1.0 ppm pipette \_\_\_\_\_ µl of clarified
extract to a labeled mico-centrifuge tube containing a 1.0 ml. DON
Dilution Buffer and mix. This is the diluted extract.

> \(a\) 1 ml (c) 100 µl
>
> \(b\) 50 µl (d) 3 drops

7\. For screening level 0.5 ppm pipette \_\_\_\_\_ µl of clarified
extract to a labeled mico-centrifuge tube containing a 1.0 ml. DON
Dilution Buffer and mix. This is the diluted extract.

> \(a\) 1 ml (c) 100 µl
>
> \(b\) 50 µl (d) 3 drops

8\. Allow the strip to incubate in the Quad incubator for 5 minutes

\(a\) True (b) False

9\. A test is invalid if a Control Line is missing, smeared or uneven,
or, if the Test Line is uneven.

\(a\) True (b) False

10\. A sample containing DON residues in excess of the screening level
will develop a Test Line that is darker in intensity than the Control
Line.

> \(a\) True (b) False

11\. The Rosa P/N test kit can be used to screen samples at 0.5 ppm and
also at 1.0 ppm.

\(a\) True (b) False

12\. Certify results as being equal to or less than screening level, as
applicable.

(a)True (b) False

**L. [Diagnostix EZ-TOX Test - Specific Questions]{.underline}**

1\. The EZ-Tox Don Kit is approved for testing barley, malted barley,
and corn from 0.5 - 2.5 ppm

\(a\) True (b) False

2\. In the extraction process place 50 grams of ground sample into a
clean plastic bag and add of distilled or deionized water and seal/close
the bag securely to prevent spillage.

\(a\) 50 ml (c) 250 ml

\(b\) 100 ml (d) 1000 ml

3\. Reagents must reach room temperature prior to running the test.

\(a\) True (b) False

4\. What is the maximum number of test samples that can be run at one
time?

\(a\) five (c) eighteen

\(b\) twelve (d) twenty

5\. Using a multi-channel pipettor add \_\_\_\_\_\_\_\_\_ µls. of
Reagent A to each well.

\(a\) 25 (c) 100

\(b\) 50 (d) 250

6\. The extraction procedure requires that the ground sample and
extraction solvent (distilled/deionized water) must be mixed by shaking
vigorously for minutes.

\(a\) two (c) four

\(b\) three (d) five

7\. After shaking the ground sample portion and extraction solvent in
the extraction process, transfer mls of sample extract to micro
centrifuge tube.

\(a\) two - three (c) one -- two

\(b\) one (d) five

8\. After the Reagent B is added to the wells the lid of the reader must
be closed and the test started quickly.

\(a\) True (b) False

9\. On the day the kit will be used it is acceptable to store the kit at
ambient room temperature (62°F to 82°F)

\(a\) True (b) False

10\. Dispense \_\_\_\_\_\_ µl of each calibrator into their assigned
wells.

\(a\) 5 (c) 15

\(b\) 25 (d) 100

11\. Allow the microplate reader to warm up to 37 ºC.

\(a\) True (b) False

12\. Unused Reagents A and B dispensed into the reagent reservoirs may
be returned to their respective bottles?

\(a\) True (b) False

M.  **[Diachemix DON FPA Test - Specific Questions]{.underline}**

1\. The extraction procedure requires that the ground sample and
extraction solvent (distilled/deionized water) must be mixed by shaking
vigorously for minutes.

\(a\) two (c) four

\(b\) three (d) five

2\. In the extraction process place 50 grams of ground sample into a
clean container and add \_\_\_\_\_\_\_\_\_\_ of distilled or deionized
water and seal/close securely to prevent spillage.

\(a\) 50 ml (c) 250 ml

\(b\) 100 ml (d) 500 ml

3\. What instrument is approved for reading test results with the
Diachemix DON FPA Test kit?

\(a\) EL-301 microwell reader (c) Stat-Fax microwell reader

\(b\) Sentry® 100 Sample Reader (d) RL-100 fluorometer

4\. The DIACHEMIX® DON FPA test kit uses fluorescence polarization assay
technology that provides quantitative results in wheat.

\(a\) True (b) False

5\. To prepare each sample for testing add 1ml of Antibody Solution and
\_\_\_\_\_of sample extract and vortex.

\(a\) 100 µl (c) 1 ml

\(b\) 0.1µl (d) 1.0 µl

6\. For Don testing, the cutoff mP value is the Control mP value plus
(+) \_\_\_\_\_\_\_\_\_.

\(a\) 100 (c) 50

\(b\) 35 (d) -25

7\. After reading 'Blank Int' for all samples, add \_\_\_\_\_ of DON-FP
Tracer to the each tube.

\(a\) 1.0 µl (c) 1 ml

\(b\) 0.1µl (d) 100 µl

8\. After adding the DON-FP Tracer to the each tube Vortex thoroughly
and incubate for \_\_\_\_\_\_\_\_\_ minutes.

\(a\) 3 (c) 1

> \(b\) 2 (d) 5

9\. Run the control and calculate the cutoff at the start of each day.
Rerun the control and recalculate the cutoff every 100 samples or if the
temperature in the lab changes by five degrees or more.

\(a\) True (b) False

10\. Report Negative results on the pan ticket and inspection log as
being equal to or less than \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\(a\) 2.0 ppm (≤ 2.0ppm) (c) 1.0 ppm (≤ 1.0 ppm).

> \(b\) 0.5 ppm (d) 5.0 ppm (≤ 5 ppm)

11\. When calibrating the reader, the instrument has failed an important
quality control check if the average for the last five 'Sample mP'
values is not greater than 360 mP.

\(a\) True (b) False

12\. The Buffer, Low Polarization Standard and High Polarization
Standard solutions may be returned to their appropriate bottles and
reused.

\(a\) True (b) False