Permits Branch (Date)

Biotechnology Permits

Biotechnology Regulatory Services

4700 River Road, Unit 147

Riverdale, MD 20737-1228

Reference No.: (this will be generated by APHIS, leave blank)

Applicant Reference No.: (Select a number meaningful to you)

Applicant/Responsible Party: (name, address, phone, fax, and e-mail of
requesting scientist)

Duration of Introduction: Interstate movement, (fill in shipment dates,
a range covering several months up to a year such as June 1, 2004 to
Sept. 30, 2004)

Recipient: Barley, Hordeum vulgare L., cultivars: Golden Promise

Regulated Article: small amounts of transformed barley seed (less than
25 seed per line), from the following lines, as described below:

a\) Category: MG, OO

Designation: (fill in ID information for all requested lines, such as
TNP-4, TNP-6, TNP-20)

Construct: pSP- Ds-ubi-bar

Phenotype: resistant to glufosinate-ammonium; Ds sequences enable Ac
transposase- mediated transposition

Genotype:

Promoter\--Maize ubiquitin

Enhancer: maize ubiquitin 1 intron

Gene: bar from *Streptomyces hygroscopicus* encoding phosphinothricin
acetyl transferase

Terminator: nopaline synthase 3\' end from Agrobacterium tumefaciens

Other: 254 bp 5\' terminal and subterminal sequence repeat and 340 bp
3\' terminal and

subterminal sequence repeat from the Ac transposable element from maize

b\) Category: MG, OO

Designation: GP Ac-AcTPase

Construct: pUC-codA-Act-Ac-Ac

Phenotype: expresses codA and Ac transposase

Genotype:

Promoter: for codA\--rice actin1; for Ac transposase, the native
promoter of transposase

Enhancer: rice actin1 intron for codA; none for Ac transposase

Gene: codA from E. coli encoding cytosine deaminase; Ac transposase from
maize

Terminator: nopaline synthase 3\' end from *Agrobacterium tumefaciens*
for both genes

Selectable marker:

Promoter: maize ubiquitin

Enhancer: maize ubiquitin 1 intron

Gene: bar from *Streptomyces hygroscopicus* encoding phosphinothricin
acetyl transferase

Terminator: nopaline synthase 3\' end from *Agrobacterium tumefaciens*

c\) Category: MG, OO

Designation: GP Ubi-AcTPase

Construct: pUC-codA-Act-Ubi-Ac

Phenotype: expresses codA and Ac transposase

Genotype:

Promoter: for codA\--rice actin1; for Ac transposase, maize ubiquitin
promoter

Enhancer: rice actin1 intron for codA; none for Ac transposase

Gene: codA from E. coli encoding cytosine deaminase; Ac transposase from
maize

Terminator: nopaline synthase 3\' end from *Agrobacterium tumefaciens*
for both genes

Selectable marker:

Promoter: maize ubiquitin

Enhancer: maize ubiquitin 1 intron

Gene: bar from *Streptomyces hygroscopicus* encoding phosphinothricin
acetyl transferase

Terminator: nopaline synthase 3\' end from *Agrobacterium tumefaciens*

Mode of Transformation: Microprojectile bombardment.

Introduction: Interstate movement

Destination: (name and address of requesting scientist)

Certification: I certify that the regulated article will be introduced
in accordance with the

eligibility criteria and the performance standards set forth in 7 CFR
340.3. The above

information in true to the best of my knowledge. If there are any
changes, I will contact APHIS.

(Signature of requesting scientist)

(Typed name of requesting scientist)