----- ------- ------------------------------------------ ---------------
                **Determine HIV Rapid Test**\              
                **(For use with whole blood, serum, or     
                plasma)\                                   
                Store kit: 2 - 30° C**                     

                                                           
  ----- ------- ------------------------------------------ ---------------

-   **Check kit before use. Use only items that have not expired or been
    damaged.**

-   **Bring kit and previously stored specimens to room temperature
    prior to use.**

-   **Always use universal safety precautions when handling specimens.
    Keep work areas clean and organized.**

**This outline is not intended to replace the product insert or your
standard operating procedure (SOP).**

+---------------------+-----------+-----------+----------------------+
| ![](media/im        | ![](m     |           | ![](media/           |
| age1.jpeg){width="1 | edia/imag |           | image3.jpeg){width=" |
| .898611111111111in" | e2.jpeg){ |           | 1.979861111111111in" |
| height="1.2         | width="1. |           | height="1.           |
| 930555555555556in"} | 979861111 |           | 2930555555555556in"} |
|                     | 111111in" |           |                      |
|                     | hei       |           |                      |
|                     | ght="1.29 |           |                      |
|                     | 305555555 |           |                      |
|                     | 55556in"} |           |                      |
+---------------------+-----------+-----------+----------------------+
| 1.  **Collect test  | 2.  **Use |           | 3.  **Label the test |
|     items and other |     1     |           |     strip with       |
|     necessary lab   |     strip |           |     client           |
|     supplies.**     |     per   |           |     identification   |
|                     |     test  |           |     number.**        |
|                     |     and   |           |                      |
|                     |     be    |           |                      |
|                     |     sure  |           |                      |
|                     |     to    |           |                      |
|                     |           |           |                      |
|                     |  preserve |           |                      |
|                     |     the   |           |                      |
|                     |     lot   |           |                      |
|                     |           |           |                      |
|                     |    number |           |                      |
|                     |     on    |           |                      |
|                     |     the   |           |                      |
|                     |           |           |                      |
|                     | remaining |           |                      |
|                     |           |           |                      |
|                     |    packet |           |                      |
|                     |     of    |           |                      |
|                     |           |           |                      |
|                     | strips.** |           |                      |
+---------------------+-----------+-----------+----------------------+
| ![](media/im        | ![](me    | ![](      | ![](media/i          |
| age4.jpeg){width="1 | dia/image | media/ima | mage7.jpeg){width="1 |
| .979861111111111in" | 5.jpeg){w | ge6.jpeg) | .9708333333333334in" |
| height="1.2         | idth="1.2 | {width="1 | height="1            |
| 930555555555556in"} | 833333333 | .19375in" | .292361111111111in"} |
|                     | 333334in" | hei       |                      |
|                     | hei       | ght="1.29 |                      |
|                     | ght="1.29 | 097222222 |                      |
|                     | 166666666 | 22222in"} |                      |
|                     | 66667in"} |           |                      |
+---------------------+-----------+-----------+----------------------+
| 4.  **Pull off the  | 5.        |           | 6.  **Apply the      |
|     protective foil | **Collect |           |     specimen to the  |
|     cover.**        |     50 µl |           |     absorbent pad on |
|                     |     of    |           |     the strip.**     |
|                     |           |           |                      |
|                     |  specimen |           |                      |
|                     |     using |           |                      |
|                     |           |           |                      |
|                     |    either |           |                      |
|                     |     a     |           |                      |
|                     |           |           |                      |
|                     |   pasteur |           |                      |
|                     |     or    |           |                      |
|                     |           |           |                      |
|                     | precision |           |                      |
|                     |     p     |           |                      |
|                     | ipette.** |           |                      |
+---------------------+-----------+-----------+----------------------+
| ![](media/im        | ![](m     |           | ![](media/i          |
| age8.jpeg){width="1 | edia/imag |           | mage10.jpeg){width=" |
| .979861111111111in" | e9.jpeg){ |           | 1.979861111111111in" |
| height="1.2         | width="1. |           | height="1.           |
| 930555555555556in"} | 979861111 |           | 2930555555555556in"} |
|                     | 111111in" |           |                      |
|                     | hei       |           |                      |
|                     | ght="1.29 |           |                      |
|                     | 305555555 |           |                      |
|                     | 55556in"} |           |                      |
+---------------------+-----------+-----------+----------------------+
| 7.  **[For whole    | 8         |           | 9.  **Read and       |
|     blood           | .  **Wait |           |     record the       |
|                     |     15    |           |     results and      |
|   only]{.underline} |           |           |     other pertinent  |
|     add 1 drop of   |   minutes |           |     info on the      |
|     chase buffer to |     (no   |           |     worksheet.**     |
|     the specimen    |           |           |                      |
|     pad.**          |    longer |           |                      |
|                     |     than  |           |                      |
|                     |     60    |           |                      |
|                     |           |           |                      |
|                     |  minutes) |           |                      |
|                     |           |           |                      |
|                     |    before |           |                      |
|                     |           |           |                      |
|                     |   reading |           |                      |
|                     |     the   |           |                      |
|                     |     r     |           |                      |
|                     | esults.** |           |                      |
+---------------------+-----------+-----------+----------------------+

**Determine HIV Rapid Test Results**

+---------------------------+------------------------------------------+
| Reactive                  | ![](media/im                             |
|                           | age11.jpeg){width="3.9784722222222224in" |
| 2 lines of any intensity  | height="0.7597222222222222in"}           |
| appear in\                |                                          |
| both the control and      |                                          |
| patient areas.            |                                          |
+---------------------------+------------------------------------------+
| Non-reactive              | ![](media/im                             |
|                           | age12.jpeg){width="3.9784722222222224in" |
| 1 line appears in the     | height="0.7597222222222222in"}           |
| control area\             |                                          |
| and no line in the        |                                          |
| patient area.             |                                          |
+---------------------------+------------------------------------------+
| Invalid                   | ![](media/image13.jpeg){width="4.0in"    |
|                           | height="0.75in"}                         |
| No line appears in the    |                                          |
| control area.\            |                                          |
| Do not report invalid     |                                          |
| results. Repeat test      |                                          |
|                           |                                          |
| with a new test device    |                                          |
| even if a line            |                                          |
|                           |                                          |
| appears in the patient    |                                          |
| area.                     |                                          |
+---------------------------+------------------------------------------+

  ------------------------------------------------------ ----------------------------------------- ------------------------------------------------------------------------------------
  ![](media/image14.jpeg){width="0.4798611111111111in"   Use of trade names and commercial sources ![](media/image15.png){width="0.39861111111111114in"
  height="0.6243055555555556in"}                         is for identification only and does not   height="0.39861111111111114in"}![](media/image16.png){width="0.6104166666666667in"
                                                         imply endorsement by WHO, the Public      height="0.40069444444444446in"}
                                                         Health Service, or by the U.S. Department 
                                                         of Health and Human Services (2005).      

  ------------------------------------------------------ ----------------------------------------- ------------------------------------------------------------------------------------