Statement of Work

1\. [Objectives]{.underline}

The contractor will provide Dr. Michael Iadarola with professional
laboratory services. Jason Keller's work will focus on biochemical and
molecular biological investigations of nervous system plasticity, gene
regulation, and ion channel function in primary cultures of nervous
system cells as well as in vivo studies using animal models.

2\. [Approach]{.underline}

The contractor will be involved in implementing state-of-the-art
live-cell microscopic imaging, cell biology and molecular biology
methods for analysis of ion channel function in vitro and induced gene
expression in spinal cord and brain during states of pharmacological,
physiological or pathophysiological activation.

3\. [Methods]{.underline}

The services that will be performed are as follows:

a\) The contractor will use measurements of fluorescence and radioactive
calcium -- 45 uptake in cultured cell and primary cultures of neurons
and glial cells and stably and transiently transfected cell lines. The
contractor will be able to manipulate plasmids and viral vectors as part
of these studies.

b\) The contractor will maintain detailed records of experimental
procedures and results. High quality laboratory notebooks are
maintained, which will be consulted to extract results and raw data for
processing into graphs for research reports and published manuscripts.

c\) Data will be analyzed using appropriate statistical approaches to
demonstrate significant differences resulting from experimental
manipulations. Familiarity with MetaMorph and other imaging software
packages is also essential.

**Personnel, evaluation and schedule**

A. [Personnel]{.underline}

The contractor has training in basic molecular biological methods such
as RNA extraction, cDNA synthesis, polymerase chain reaction (PCR),
cloning of DNA fragments and cDNAs and subcloning into plasmids and
various forms of differential hybridization. The contractor is capable
of performing experiments using live cell imaging techniques and has
familiarity with live cell imaging techniques using flow through
chambers, microscopy, video and CCD camera recording and use of
MetaMorph and other imaging software packages as necessary for data
acquisition and analysis.

Personnel provided under the contract will comply with the rules and
policies of the NIDCR and its staff.

B. [Evaluation]{.underline}

The contractor will be evaluated daily and if the contractor is
considered to be substandard in the judgment of the supervisor, the
contractor will initially receive notice of the problem from the
supervisor and will be required to take corrective measures and to
demonstrate improved performance.

C. [Schedule]{.underline}

The contractor will perform studies for a minimum of 40 hours per week,
Monday through Friday (except any federal holidays). Failure to arrive
on time or to follow the time schedules or instructions as indicated
shall be considered default of the contract.

D. [Reporting Requirements]{.underline}

The contractor shall provide professional services for Neurobiology and
Pain Therapeautics, NIDCR during the period of April 2, 2007 to
September 28, 2007. The contractor will be paid a total of \$27, 552.

**Justification**

Attached is a request for \$27,552.00 to renew the contract services of
Jason Keller. Under this contract, Jason Keller will provide services to
the Neurobiology and Pain Therapeautics, NIDCR. His contract will run
from April 2, 2007 to September 28, 2007.

These are highly specialized technical procedures and Mr. Keller has all
of the technical skills and practical training to execute the procedures
in a high quality conscientious fashion. The procedures require a great
deal of personal attention to detail, cleanliness and knowledge of
sterile tissue culture technique, good skills with microscopic
microsurgery, good skills with computer databases, statistical analyses,
and practical experience with robotic liquid handling devices,
luminometers and various UV and fluorescent plate readers. Without
someone filling this position, the productivity of our laboratory will
be less than optimal. Considering all of his skills, Mr. Keller is the
ideal candidate for this position.

[Task Description]{.underline}

Firm will issue 13 checks on the following days for the following
amounts:

April 13th for \$2016.00

April 27^th^ for \$2016.00

May 11^th^ for \$2240.00

May 25th^th^ for \$2240.00

June 8^th^ for \$2016.00

June 22^nd^ for \$2240.00

July 6^th^ for \$2240.00

July 20^th^ for \$2016.00

August 3rd for \$2240.00

August 17^th^ for \$2240.00

August 31^st^ for \$2016.00

September 14^th^ for \$1792.00

September 28^th^ for \$2240.00

Total = \$27,552.

Checks will be issued Jason Keller. A total of \$27,552 shall be paid in
13 payments. The check should be sent via express mail to the NIDCR, to
the attention of Michael Iadarola at the following address.

Dr. Michael J. Iadarola

Building 49, Room 1A04

National Institutes of Health

49 Convent Drive MSC 4410

Bethesda, MD 20892-4410

Telephone: 301-402-4947

Fax: 301-480-2012

Preparer, Jacinta Mason, NIDCR