**OZONE BIOINDICATOR PLANTS -- 2004 - SITE CHARACTERISTICS**

####### [Crew Reminder: Take this sheet to the Biosite! Complete it in the field!]{.underline}

#### [2004 TRAINING AND CERTIFICATION EXERCISE]{.mark}

**To be filled out by the FIELD CREW or Cooperator: Refer to Field Guide
1.6 for code definitions.**

+----+-----+----------------+---------+-----+---+---------+--------+
| *  | *   | **Ozone        | **Plot  | **M | * | **Crew  | **Crew |
| *S | *Co | Hexagon        | Numb    | ont | * | ID**    | Type** |
| ta | unt | Number**       | er^1^** | h** | D |         |        |
| te | y** |                |         |     | a |         |        |
| ** |     |                |         |     | y |         |        |
|    |     |                |         |     | * |         |        |
|    |     |                |         |     | * |         |        |
+----+-----+----------------+---------+-----+---+---------+--------+
|    |     |                |         |     |   |         | **    |
|    |     |                |         |     |   |         | reg    |
|    |     |                |         |     |   |         | ular** |
|    |     |                |         |     |   |         |        |
|    |     |                |         |     |   |         | **    |
|    |     |                |         |     |   |         | QA**   |
+----+-----+----------------+---------+-----+---+---------+--------+

**^1^Plot Number refers to the number of locations (1 or 2) used for
each hexagon number. A separate sheet should be used for each
location.**

**√ Please put a check mark beside the correct information. Please
complete all data fields.**

  --------- ------------------------------------------------------------------
  **Ozone   
  Sample    
  Kind:**   

            **Initial plot establishment on the 2002 FIA ozone grid.**

            **Remeasurement of a previously established plot.**

            **Replacement of a previously established plot that was replaced
            to meet new site selection guidelines (or lost site).**
  --------- ------------------------------------------------------------------

  -------------- ------------------------------------------------------------------
  **Ozone Grid   
  Density: (Is   
  the grid       
  intensified,   
  or not?)**     

                 **This hex number identifies a unique ozone plot within a polygon
                 (1 site:1 polygon)**

                 **One of two or more ozone plots within the same polygon, each
                 with their own hexagon number.**
  -------------- ------------------------------------------------------------------

  --------- ------------------------- ------------- ------------------------------
  **Plot                              **Terrain     
  size:**                             position:**   

            **\> 1.2 hectares (3.0                  **Ridge top or upper slope**
            acres)**                                

            **0.2 -- 1.2 hectares                   **Bench or level area along a
            (1/2 to 3 acres)**                      slope**

            **Other: please                         **Lower slope**
            describe**                              

                                                    **Flat land unrelated to
                                                    slope**

                                                    **Bottom land with occasional
                                                    flooding**
  --------- ------------------------- ------------- ------------------------------

  -------------------------------- ------------------- -------------------
  **Aspect: 000° = no aspect; 360° **Elevation: record 
  = N aspect**                     estimate in feet or 
                                   meters**            

  **Record to nearest degree =**   **Feet =**          **Meters =**
  -------------------------------- ------------------- -------------------

  ------------- ---------------------------- ---------- ---------------------------------
  **Soil                                     **Soil     
  Drainage:**                                Depth:**   

                **Well-drained**                        **Bedrock not exposed**

                **Wet**                                 **Bedrock exposed**

                **Excessively dry**                     
  ------------- ---------------------------- ---------- ---------------------------------

+---+------------------------------------------------------------------+
| # |                                                                  |
|   |                                                                  |
| D |                                                                  |
| i |                                                                  |
| s |                                                                  |
| t |                                                                  |
| u |                                                                  |
| r |                                                                  |
| b |                                                                  |
| a |                                                                  |
| n |                                                                  |
| c |                                                                  |
| e |                                                                  |
| : |                                                                  |
|   |                                                                  |
| D |                                                                  |
| i |                                                                  |
| s |                                                                  |
| t |                                                                  |
| u |                                                                  |
| r |                                                                  |
| b |                                                                  |
| a |                                                                  |
| n |                                                                  |
| c |                                                                  |
| e |                                                                  |
|   |                                                                  |
| o |                                                                  |
| n |                                                                  |
|   |                                                                  |
| t |                                                                  |
| h |                                                                  |
| e |                                                                  |
|   |                                                                  |
| s |                                                                  |
| i |                                                                  |
| t |                                                                  |
| e |                                                                  |
|   |                                                                  |
| o |                                                                  |
| r |                                                                  |
|   |                                                                  |
| i |                                                                  |
| n |                                                                  |
|   |                                                                  |
| l |                                                                  |
| o |                                                                  |
| c |                                                                  |
| a |                                                                  |
| l |                                                                  |
| i |                                                                  |
| z |                                                                  |
| e |                                                                  |
| d |                                                                  |
|   |                                                                  |
| a |                                                                  |
| r |                                                                  |
| e |                                                                  |
| a |                                                                  |
| s |                                                                  |
|   |                                                                  |
| w |                                                                  |
| h |                                                                  |
| e |                                                                  |
| r |                                                                  |
| e |                                                                  |
|   |                                                                  |
| t |                                                                  |
| h |                                                                  |
| e |                                                                  |
|   |                                                                  |
| b |                                                                  |
| i |                                                                  |
| o |                                                                  |
| i |                                                                  |
| n |                                                                  |
| d |                                                                  |
| i |                                                                  |
| c |                                                                  |
| a |                                                                  |
| t |                                                                  |
| o |                                                                  |
| r |                                                                  |
|   |                                                                  |
| p |                                                                  |
| l |                                                                  |
| a |                                                                  |
| n |                                                                  |
| t |                                                                  |
| s |                                                                  |
|   |                                                                  |
| a |                                                                  |
| r |                                                                  |
| e |                                                                  |
|   |                                                                  |
| g |                                                                  |
| r |                                                                  |
| o |                                                                  |
| w |                                                                  |
| i |                                                                  |
| n |                                                                  |
| g |                                                                  |
| . |                                                                  |
+---+------------------------------------------------------------------+
| # | # No recent or                                                   |
|   | significant disturbance; Do not count disturbance \>3 years old. |
+---+------------------------------------------------------------------+
| # | # Evidence of ove                                                |
|   | ruse; Human activity causing obvious soil compaction or erosion. |
+---+------------------------------------------------------------------+
| # | # Evidence of natural                                            |
|   | disturbance including fire, wind, flooding, grazing, pests, etc. |
+---+------------------------------------------------------------------+

**Fill in below all that apply. Check here if geographic coordinates
were obtained from a topographic map:**

  ----------------------------------- -----------------------------------
  **GPS Type:**                       **GPS Serial Number:**

  **Latitude =**                      **GPS Error =**

  **Longitude =**                     **Number of Readings =**

  **Elevation =**                     **GPS File Name =**
  ----------------------------------- -----------------------------------

**^1^If no GPS Unit is available, please use a map and record estimated
latitude, longitude, and elevation for each plot location.**

**Comments: Include information on additional species in the area,
safety, directions, or additional site characteristics that may be
useful.**

**File this completed data sheet with the sheet used for mapping the
Bioindicator Site Location and then store it in the appropriate Ozone
Plot Folder for your State or Region.**

### OZONE BIOINDICATOR PLANTS

####### Data Sheet for Mapping the Bioindicator Site Location

#### [2004 TRAINING AND CERTIFICATION EXERCISE]{.mark}

**To be filled out by the FIELD CREW or Cooperator: Refer to Field Guide
1.6 for code definitions.**

  ----------- ------------ ------------------ ------------- ----------- --------- ---------- -------------
  **State**   **County**   **Ozone Hexagon    **Plot        **Month**   **Day**   **Year**   **Crew ID**
                           Number**           Number^1^**                                    

                                                                                             
  ----------- ------------ ------------------ ------------- ----------- --------- ---------- -------------

**^1^Plot Number refers to the number of locations (1 or 2) used for
each hexagon number. A separate sheet should be used for each
location.**

**Include the following information on the map. Use a legend that
indicates distances between obvious points on the map.**

(1) **Location of the site relative to some obvious and permanent
    marker.**

(2) **Road names and distances as needed.**

(3) **North arrow.**

(4) **Species codes and approximate location of plant groupings used for
    the ozone injury evaluations.**

(5) **Starting point**

(6) **Location of and distance to two major roads**

(7) **Distance and direction to two major towns**

(8) **Gazetteer reference page if available.**

Return the original of this map to the corresponding Plot Folder so it
can be used by audit and regular crews in subsequent visits to the plot.
Mail a copy to the National Indicator Advisor the year that the site is
established.

  ----------------------------------- -----------------------------------
  **Geographic coordinates:**         

  **GPS Latitude =**                  **GPS Longitude =**

  **Latitude estimated from a         
  topographic map =**                 

  **Longitude estimated from a        
  topographic map =**                 
  ----------------------------------- -----------------------------------

#### []{.mark}

#### [REDRAW THE MAP AND ADD NEW INFORMATION EACH YEAR AS NEEDED!]{.mark}

**OZONE BIOINDICATOR PLANTS - 2004**

[**Data Sheet for the** **Voucher Leaf Samples**]{.underline}

[2004 TRAINING AND CERTIFICATION EXERCISE]{.mark}

**To be filled out by the FIELD CREW or Cooperator: Refer to the Ozone
Field Guide for code definitions.**

+----+-----+----------------+---------+-----+---+-------+----------+
| *  | *   | **Ozone        | **Plot  | **M | * | *     | **Crew   |
| *S | *Co | Hexagon        | Numb    | ont | * | *Crew | Type**   |
| ta | unt | Number**       | er^1^** | h** | D | ID**  |          |
| te | y** |                |         |     | a |       |          |
| ** |     |                |         |     | y |       |          |
|    |     |                |         |     | * |       |          |
|    |     |                |         |     | * |       |          |
+----+-----+----------------+---------+-----+---+-------+----------+
|    |     |                |         |     |   |       | **      |
|    |     |                |         |     |   |       | r        |
|    |     |                |         |     |   |       | egular** |
|    |     |                |         |     |   |       |          |
|    |     |                |         |     |   |       | ** QA** |
+----+-----+----------------+---------+-----+---+-------+----------+

**^1^Plot Number refers to the number of locations (1 or 2) used for
each hexagon number. Separate sheets should be used for each location.**

**Fill in the required codes. ONE SPECIES PER LINE. Code definitions are
in the Field Guide.**

+---+----------+------+-----+-----------------------------------------+
| B |          | In   | Inj | Is the leaf sample injury close to 100% |
| i |          | jury | ury | ozone stipple (**√)** , or is some      |
| o |          |      |     | other upper-leaf-surface injury also    |
| i |          | Loca | T   | present (e.g., insect injury or fungal  |
| n |          | tion | ype | lesions)?                               |
| d |          |      |     |                                         |
| i |          |      |     |                                         |
| c |          |      |     |                                         |
| a |          |      |     |                                         |
| t |          |      |     |                                         |
| o |          |      |     |                                         |
| r |          |      |     |                                         |
| S |          |      |     |                                         |
| p |          |      |     |                                         |
| e |          |      |     |                                         |
| c |          |      |     |                                         |
| i |          |      |     |                                         |
| e |          |      |     |                                         |
| s |          |      |     |                                         |
| C |          |      |     |                                         |
| o |          |      |     |                                         |
| d |          |      |     |                                         |
| e |          |      |     |                                         |
| o |          |      |     |                                         |
| r |          |      |     |                                         |
| C |          |      |     |                                         |
| o |          |      |     |                                         |
| m |          |      |     |                                         |
| m |          |      |     |                                         |
| o |          |      |     |                                         |
| n |          |      |     |                                         |
| N |          |      |     |                                         |
| a |          |      |     |                                         |
| m |          |      |     |                                         |
| e |          |      |     |                                         |
+---+----------+------+-----+-----------------------------------------+
| 1 |          |      |     | Close to 100% \_\_\_\_\_\_ Estimated    |
| ^ |          |      |     | percent other \_\_\_\_\_\_\_            |
| s |          |      |     |                                         |
| t |          |      |     |                                         |
| ^ |          |      |     |                                         |
+---+----------+------+-----+-----------------------------------------+
| 2 |          |      |     | Close to 100% \_\_\_\_\_\_ Estimated    |
| ^ |          |      |     | percent other \_\_\_\_\_\_\_            |
| n |          |      |     |                                         |
| d |          |      |     |                                         |
| ^ |          |      |     |                                         |
+---+----------+------+-----+-----------------------------------------+
| 3 |          |      |     | Close to 100% \_\_\_\_\_\_ Estimated    |
| ^ |          |      |     | percent other \_\_\_\_\_\_\_            |
| r |          |      |     |                                         |
| d |          |      |     |                                         |
| ^ |          |      |     |                                         |
+---+----------+------+-----+-----------------------------------------+
| 4 |          |      |     | Close to 100% \_\_\_\_\_\_ Estimated    |
| ^ |          |      |     | percent other \_\_\_\_\_\_\_            |
| t |          |      |     |                                         |
| h |          |      |     |                                         |
| ^ |          |      |     |                                         |
+---+----------+------+-----+-----------------------------------------+

**[Species codes:]{.underline}** **[Injury Location
codes:]{.underline}**

915 Blackberry 1 = greater than 50% of the injured leaves are younger
leaves;

762 Black cherry 2 = greater than 50% of the injured leaves are mid-aged
or older;

365 Milkweed 3 = injured leaves are all ages.

621 Yellow poplar

541 White ash **[Injury type codes:]{.underline}**

931 Sassafras 1 = greater than 50% of the injury is upper-leaf-surface
stipple;

611 Sweetgum 2 = greater than 50% is not stipple (tan flecks, bifacial
or general discoloration);

761 Pin cherry 3 = injury is varied or difficult to describe.

366 Spreading dogbane

364 Bigleaf aster.

999 Unknown

111 Supplemental

(write out common name)

**Mail this sheet with the leaf samples to:**

**QA/QC PERSON: To be filled out by the National Ozone Advisor or
Regional Expert. √**

+---------+----------+-------------+-------+-------------+-----+-----+
| Date    | Date     | Sample      |       |             | P   |     |
| checked | r        | condition   |       |             | lot |     |
|         | echecked |             |       |             | Sta |     |
|         |          |             |       |             | tus |     |
+---------+----------+-------------+-------+-------------+-----+-----+
|         |          | GOOD        | FAIR  | POOR        | (+  | (-  |
|         |          |             |       |             | ozo | ozo |
|         |          | easy to     |       | samples     | ne) | ne) |
|         |          | read - ID   |       | unreadable  |     |     |
|         |          | obvious     |       | or not      |     |     |
|         |          |             |       | labeled     |     |     |
|         |          |             |       | correctly   |     |     |
+---------+----------+-------------+-------+-------------+-----+-----+

+--------+-------+-------+-------------------------------------------+
| Bioind | Pos   | Neg   | Explanation                               |
| icator | itive | ative |                                           |
| S      |       | for   |                                           |
| pecies | for   | ozone |                                           |
|        | ozone |       |                                           |
+--------+-------+-------+-------------------------------------------+
|        |       |       |                                           |
+--------+-------+-------+-------------------------------------------+
|        |       |       |                                           |
+--------+-------+-------+-------------------------------------------+
|        |       |       |                                           |
+--------+-------+-------+-------------------------------------------+
|        |       |       |                                           |
+--------+-------+-------+-------------------------------------------+