**[Example of a logbook for quality control of stains]{.underline}**

+-------------+-------------+-------------+-------------+-------------+
| Batches     |             |             |             |             |
| checked on  |             |             |             |             |
| date        |             |             |             |             |
| 5/1/02:     |             |             |             |             |
|             |             |             |             |             |
| Ca          |             |             |             |             |
| rbolfuchsin |             |             |             |             |
| 5/1/02,     |             |             |             |             |
| Sulphuric   |             |             |             |             |
| acid        |             |             |             |             |
| 5/1/02,     |             |             |             |             |
| Methylene   |             |             |             |             |
| blue 5/1/02 |             |             |             |             |
|             |             |             |             |             |
| Average     |             |             |             |             |
| grading     |             |             |             |             |
| positive    |             |             |             |             |
| controls:   |             |             |             |             |
| no. 345 =   |             |             |             |             |
| 30/100      |             |             |             |             |
| fields ;    |             |             |             |             |
| no. 411 =   |             |             |             |             |
| 22/100      |             |             |             |             |
| fields      |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Control     | AFB colour  | AFB number  | Destaining  | Remarks     |
| Slide       |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| 345/12      | strong red  | 20/100 F    | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| 411/25      | strong red  | 50/100 F    | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | NA          | none        | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | NA          | none        | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Batches     |             |             |             |             |
| checked on  |             |             |             |             |
| date        |             |             |             |             |
| 15/1/02:    |             |             |             |             |
|             |             |             |             |             |
| Ca          |             |             |             |             |
| rbolfuchsin |             |             |             |             |
| 15/1/02,    |             |             |             |             |
| Sulphuric   |             |             |             |             |
| acid        |             |             |             |             |
| 15/1/02 (+  |             |             |             |             |
| own         |             |             |             |             |
| methylene   |             |             |             |             |
| blue        |             |             |             |             |
| working     |             |             |             |             |
| solution)   |             |             |             |             |
|             |             |             |             |             |
| Average     |             |             |             |             |
| grading     |             |             |             |             |
| positive    |             |             |             |             |
| controls:   |             |             |             |             |
| no. 345 =   |             |             |             |             |
| 30/100      |             |             |             |             |
| fields ;    |             |             |             |             |
| no. 411 =   |             |             |             |             |
| 22/100      |             |             |             |             |
| fields      |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Control     | AFB colour  | AFB number  | Destaining  | Remarks     |
| Slide       |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| 345/13      | weak red    | 2/100 F     | OK          | Reject      |
+-------------+-------------+-------------+-------------+-------------+
| 411/26      | weak red    | 0/100 F     | OK          | Reject      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | NA          | none        | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | NA          | none        | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Note: this  |             |             |             |             |
| batch of    |             |             |             |             |
| ca          |             |             |             |             |
| rbolfuchsin |             |             |             |             |
| is bad, all |             |             |             |             |
| has been    |             |             |             |             |
| discarded.  |             |             |             |             |
| Sulphuric   |             |             |             |             |
| is OK.      |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Batches     |             |             |             |             |
| checked on  |             |             |             |             |
| 16/2/02:    |             |             |             |             |
|             |             |             |             |             |
| Ca          |             |             |             |             |
| rbolfuchsin |             |             |             |             |
| 16/1/02,    |             |             |             |             |
| Methylene   |             |             |             |             |
| blue        |             |             |             |             |
| 16/1/02 (+  |             |             |             |             |
| own         |             |             |             |             |
| sulphuric   |             |             |             |             |
| acid        |             |             |             |             |
| working     |             |             |             |             |
| solution)   |             |             |             |             |
|             |             |             |             |             |
| Average     |             |             |             |             |
| grading     |             |             |             |             |
| positive    |             |             |             |             |
| controls:   |             |             |             |             |
| no. 345 =   |             |             |             |             |
| 30/100      |             |             |             |             |
| fields ;    |             |             |             |             |
| no. 411 =   |             |             |             |             |
| 22/100      |             |             |             |             |
| fields      |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Control     | AFB colour  | AFB number  | Destaining  | Remarks     |
| Slide       |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| 345/14      | strong red  | 34/100 F    | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| 411/27      | strong red  | 40/100 F    | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | strong red  | 3 clumps    | OK          | Reject      |
+-------------+-------------+-------------+-------------+-------------+
| NEG         | NA          | None        | OK          | Accept      |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Note:       |             |             |             |             |
| con         |             |             |             |             |
| tamination, |             |             |             |             |
| probably    |             |             |             |             |
| methylene   |             |             |             |             |
| blue. To be |             |             |             |             |
| checked     |             |             |             |             |
| further     |             |             |             |             |
| using only  |             |             |             |             |
| 1 of the    |             |             |             |             |
| new stains  |             |             |             |             |
| on negative |             |             |             |             |
| controls (+ |             |             |             |             |
| old good    |             |             |             |             |
| stains of   |             |             |             |             |
| the other   |             |             |             |             |
| types).     |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+

+-------------+-------------+-------------+-------------+-------------+
| Batches     |             |             |             |             |
| checked on  |             |             |             |             |
| : ........  |             |             |             |             |
|             |             |             |             |             |
| Ca          |             |             |             |             |
| rbolfuchsin |             |             |             |             |
| da          |             |             |             |             |
| te......... |             |             |             |             |
| Co          |             |             |             |             |
| ncentration |             |             |             |             |
| ......      |             |             |             |             |
| ........... |             |             |             |             |
|             |             |             |             |             |
| Auramine    |             |             |             |             |
| date        |             |             |             |             |
| .           |             |             |             |             |
| ........... |             |             |             |             |
| Co          |             |             |             |             |
| ncentration |             |             |             |             |
| ........    |             |             |             |             |
| .......\... |             |             |             |             |
|             |             |             |             |             |
| Decolorizer |             |             |             |             |
| date        |             |             |             |             |
| ........... |             |             |             |             |
| Type        |             |             |             |             |
| ..........  |             |             |             |             |
| ........... |             |             |             |             |
|             |             |             |             |             |
| Methylene   |             |             |             |             |
| date        |             |             |             |             |
| .           |             |             |             |             |
| ........... |             |             |             |             |
| Co          |             |             |             |             |
| ncentration |             |             |             |             |
| ........... |             |             |             |             |
| ........... |             |             |             |             |
|             |             |             |             |             |
| P           |             |             |             |             |
| ermanganate |             |             |             |             |
| date        |             |             |             |             |
| ..          |             |             |             |             |
| ........... |             |             |             |             |
| Co          |             |             |             |             |
| ncentration |             |             |             |             |
| ........... |             |             |             |             |
| ........... |             |             |             |             |
|             |             |             |             |             |
| Average     |             |             |             |             |
| grading     |             |             |             |             |
| positive    |             |             |             |             |
| controls =  |             |             |             |             |
| ....        |             |             |             |             |
| ........AFB |             |             |             |             |
| / 100       |             |             |             |             |
| fields      |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
| Control     | AFB colour  | AFB number  | Destaining  | Remarks     |
| Slide ID    |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+
|             |             |             |             |             |
+-------------+-------------+-------------+-------------+-------------+

  -------------- -------------- -------------- -------------- --------------
  **Slide No.**  **Smear size** **Smear        **Smear        **Staining**
                                thickness**    evenness**     

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              

                                                              
  -------------- -------------- -------------- -------------- --------------