Proceedings of the:

![](media/image1.emf)![](media/image2.emf)

**29^th^ Annual Eastern**

**Fish Health Workshop**

Rocco C. Cipriano, editor

March 22-26, 1999

Royal Pavillion Resort And Conference Center

Atlantic Beach, North Carolina

![](media/image3.emf)

Sponsored by:

**National Fish Health Research Laboratory**

U.S. Geological Survey - Biological Resources Division

11649 Leetown Road

Kearneysville, West Virginia 25430

[Editorial Note]{.underline}

The staff of the National Fish Health Research Laboratory welcomes you
to the 29^th^ Annual Eastern Fish Health Workshop at the Royal Pavillion
Resort and Conference Center on Atlantic Beach. Twenty-nine years ago, a
small group of federal researchers gathered on the Eastern Shore of
Maryland to share their research, discuss problems, plan new studies,
and enjoy the fellowship of each other. That first meeting was
originally inspired by Dr. Stanislaus Snieszko of the Eastern Fish
Disease Laboratory (predecessor to the National Fish Health Research
Laboratory) of the Department of the Interior and Drs. Carl Sinderman
and Aaron Rosenfeld of the National Marine Fisheries Service Laboratory
of the Department of Commerce at Oxford, Maryland. Both laboratories
were somewhat close geographically and both researched problems
associated with fish disease, but their emphasis differed between the
freshwater and marine environment. The early workshops were a focal
point that fostered interactions between the two facilities and provided
staff members with opportunities to plan and conduct collaborative
studies. Within a few short years, fish disease research became well
established in the graduate programs of several major universities in
the eastern United States and participation in the workshop evolved
beyond its original intent. Although the title of the workshop appears
to be exclusive for fish, the workshop actually encourages much broader
participation in all aspects of aquatic animal health from shellfish to
mammals. Furthermore, the workshop remains true to its roots and
encourages contributions from researchers, resource managers,
biologists, and university personnel working within both marine and
freshwater environments. Even though the Workshop has occasionally been
held in conjunction with other organizations such as the Fish Health
Section of the American Fisheries Society and the International
Association of Aquatic Animal Medicine, it is not affiliated with any
parent organization and the meeting is an entity unto itself. Today, the
National Fish Health Research Laboratory is extremely proud to continue
the tradition of the Eastern Fish Health Workshop and serve as its
annual host. We hope that you enjoy the meeting and the beauty of the
local surroundings, here on the Crystal Coast of North Carolina.

##### Rocco C. Cipriano

# 

#  

# [Monday, March 22]{.underline}

5:00 **Evening Registration And Welcome Reception**

> Hospitality room: Seasonal fruit display with yogurt dip, vegetable
> crudite with ranch dip, international cheese display with cracker,
> beer, chees and soft drinks

8:00 **Registration Adjourns**

# 

# [Tuesday, March 23]{.underline}

7:00 **Breakfast**

> Assorted juices, fresh fruit bowl, scrambled eggs, bacon, sausage,
> breakfast potatoes, grits, French toast with syrup, cold cereals,
> bakery basket, butter, preserves, coffee, hot tea, milk

8:15 Panek Welcome

# 

# [Session I:]{.underline} Bacterial Disease In Fish

# [Clifford E. Starliper]{.underline}, moderator

National Fish Health Research Laboratory, Kearneysville, WV

8:30 Cipriano Evaluation Of Single Contact Applications Of Iodophor
Against 1

*Aeromonas salmonicida*, Cause Of Furunculosis

8:45 Bader A Polymerase Chain Reaction Assay To Monitor Channel Catfish
2

> (*Ictalurus punctatus)* Eggs For *Flavobacterium columnare*

9:00 Welch Isolation And Characterization Of A Bacteriophage Which
Infects 3

> *Yersinia ruckeri*

9:15 Pieper Mycobacteriosis, Is It Truly A Chesapeake Bay Disease Or
East 4

> Coast Problem

9:30 Gauthier Detection Of Mycobacteria In Paraffin-Embedded,
Formalin-Fixed 5

> Tissues Of Striped Bass (*Morone saxatilis*) With Peptide Nucleic
>
> Acid (PNA) Probes

9:45 Serfling Evaluating The Effects Of Oxytetracycline On The
Microflora 6

> Of A Recirculating Aquaculture System

**10:00 Morning Break**

**[Session II:]{.underline} Unusual Diagnostic Cases**

**[Mohamed Faisal]{.underline}, moderator**

Michigan State University, East Lansing, MI

10:30 Wolf Mystery Of Epizootic Oral Tumors In Spring Chinook Salmon 7

10:40 McLaughlin Coral Mortalities Along The Kenyan Coast Associated
With 8

> Extensive Tissue Lysis: Possible Mycotic Etiology

10:50 Marcquenski Catch And Release: The Importance Of TLC 9

11:00 Eissa First Record Of *Renibacterium salmoninarum* Isolation From
The 10

> Sea Lamprey *(Petromyzon marinus)*

11:10 Bowser A Case Of Helminth Parasitism In Fish In A Small
Impoundment 11

11:20 Mitchell An Unusual Case: Walleye Lesions in the Visceral Cavity
12

# 

# 11:30 Marcquenski A Serendipitous Finding While Screening Spotted Musky 13

# for *Piscirickettsia* sp.

11:40 Faisal Abnormal Growth Associated With An Intracellular Bacterium
14

> In The Yellow Perch (*Perca flavescens*)

11:50 **Open Discussion**

12:00 **Lunch**

London broil with Bordelaise sauce, parslied potatoes, steamed broccoli,
fresh baked bread, death by chocolate, Iced tea and coffee

**[Session III:]{.underline} Largemouth Bass Virus Disease**

> **[John Grizzle]{.underline}, moderator**

Auburn University, Auburn, AL

1:30 Grizzle Introduction To Largemouth Bass Virus 15

1:45 Bakal Development Of Non-Lethal Testing Methods For Largemouth 16

> Bass Virus

2:00 Goldberg Host, Pathogen And Environmental Factors That Affect The
17

> Susceptibility Of Largemouth Bass To Largemouth Bass Virus

2:15 Gaskin Application And Implication Of Serology For Largemouth Bass
18

> Virus Infection Of Largemouth Bass (*Micropterus salmoides*)

2:30 Faisal Expansion Of Largemouth Bass Virus Range In Michigan's Lower
19

> Peninsula Lakes

2:45 **Open Discussion**

**3:00 Afternoon Break**

**[Session IV:]{.underline} Emergent Health Concerns Of Ornamental And
Tropical Fishes**

## 

## [Roy Yanong]{.underline}, moderator

University of Florida, Ruskin, FL

3:30 Yanong Cryptobiosis In Cichlidae: Fun For The Whole Family 20

3:45 Neiffer Fusariomycosis In Captive Parrotfish (Family *Scaridae*)
And 21

> Bonnethead Sharks (*Sphyrna tiburo*)

4:00 Lewbart Surgical Cases In Ornamental Fish: A Chance To Heal With
Cold 22

> Hard Steel

4:15 Mauel Piscirickettsiosis-Like Syndrome In Tilapia 23

4:30 Murphy Whole Blood and Plasma Cholinesterase Levels In Normal Koi
24

> (*Cyprinus carpio*)

4:45 Elsayed Ichthyophthiriasis: Atypical Outbreak In Two Susceptible
Ornamental 25

> Fish Species In Egypt Under The Same Environmental Conditions

5:00 **Adjourn**

**Dinner On Your Own**

7:30 **[Ghost Tour:]{.underline} Old Burying Grounds, Beaufort**

> (Ticket purchase required)

### Wednesday, March 24

7:00 **Breakfast**

> Assorted juices, fresh fruit bowl, scrambled eggs, bacon, sausage,
> breakfast potatoes, grits, French toast with syrup, cold cereals,
> bakery basket, butter, preserves, coffee, hot tea, milk

# [Session V:]{.underline} Emergent Issues Associated With Commercial Aquaria

**[Scott Weber]{.underline}, moderator**

New England Aquarium, Boston, MA

8:15 Weber From Wild To Exhibit: Notes On Caribbean Collecting, Shipment
26

> And Quarantine

8:30 Arnold Diagnostic Microbiology For Small Laboratories: A Practical
Guide 27

> For The Identification Of Bacterial Isolates From Fish

8:45 Frasca Molecular Approaches To The Diagnosis Of Infectious Disease
28

> Concerns In Commercial Aquaria

9:00 Harms Apatite Concretions In Spiral Valve Of Yellow Stingray
(*Urobatis* 29

> *\[Urolophus\]* *Jamaicensis*) Fetal Pups

9:15 Goodridge Problems With Protozoa: Treating A Large Reef Tank For 30

> *Cryptocaryon irritans*

9:30 Arnold Characterization Of *Photobacterium damselae* Subspecies
*damselae* 31

> Isolates From Teleosts And Elasmobranchs Housed In An Aquarium Exhibit

9:45 **Open Discussion**

10:00 **Morning Break**

**[Session VI:]{.underline}** **Myxozoa, Molluscs And Crustacea**

> **[E. Jay Lewis]{.underline}, moderator**
>
> Center for Coastal Environmental Health and Biomolecular Research
> Cooperative Oxford Laboratory, Oxford, MD

10:30 Khoo Further Investigation Into The Brain Parasite In Yellow Perch
32

10:45 Beauchamp The Discovery Of Four Cryptic Species Of Oligochaete
Hosts 33

> (*Tubifex tubifex*) Provides Insights Into The Spread Of *Myxobolus
> cerebralis* Among Wild Trout Populations

11:00 Burreson *Bonamia* Sp. (Haplosporidia) Found In Non-Native
Oysters, 34

> *Crassostrea ariakensis*, In Bogue Sound, North Carolina

11:15 McLaughlin Protozoan Infections of the Eastern Oyster
(*Crassostrea virginica*) 35

> in the Upper Chesapeake Bay: A Potential Ecological Forecast

11:30 Burnett The Depression Of Oxygen Uptake In Blue Crabs In Response
To 36

> Bacterial Challenge

11:45 Kapareiko Effects Of Stressors On Mortality Rates In Lobsters: A
Controlled 37

> Laboratory Study

12:00 **Lunch**

Layfayette crabcakes with Remoulade sauce, saffron rice, sugar snap
peas, fresh baked bread, Key Lime pie, iced tea, and coffee

**[Session VII:]{.underline}** **Trends In Aquatic Animal Diagnostics**

**[Stephen L. Kaattari]{.underline}, moderator**

Virginia Institute of Marine Science, Gloucester Point, VA

1:30 Kaattari Immunodiagnostics In Theory And Practice 38

1:45 Reece Molecular Diagnostics In Theory And Practice 39

2:00 Burreson Issues Associated With An OIE Reference Laboratory For 40

> Shellfish Disease Diagnosis

2:15 Warg USDA/APHIS And Veterinary Diagnostics 41

2:30 Barbash Standardization And Implementation Of Molecular Diagnostics
42

> Used In The United States Fish And Wildlife Service's National
>
> Fish Health Program

2:45 **Open** **Discussion**

3:00 **Afternoon Break**

**[Session VIII:]{.underline} Health And Disease Of Coral Reefs**

**[Laurie Richardson]{.underline}, moderator**

#  Florida International University, Miami, FL

3:30 Richardson Species Specific Molecular Probe Investigations Of
\"White\" 43

> Diseases Of Corals

3:45 Rothenberger Assessment of bacterial communities associated with
white 44

> plague-Infected *Montastraea annularis* corals from St Croix

4:00 Mills Amplicon Length Heterogeneity - A Tool To Investigate Coral
45

> Microbial Communities

4:15 Smith Recent Studies On Yellow Band Disease Of Scleractinian Corals
46

4:30 Gil-Agudelo Differential Response Of Zooxanthellae To Bacterial
Toxins That 47

> Produce Bacterial Bleaching In Stony Corals

4:45 Sutherland Histopathology Of The White Pox Disease Of The Caribbean
48

> Elkhorn Coral *Acropora palmate*

5:00 McLaughlin Update On The International Registry Of Coral Pathology
49

5:15 Woodley Shifting the Paradigm For Coral Reef Health Assessment 50

5:30 **Adjourn**

**Dinner On Your Own**

### Thursday, March 25

7:00 **Breakfast**

> Assorted juices, fresh fruit bowl, scrambled eggs, bacon, sausage,
> breakfast potatoes, grits, French toast with syrup, cold cereals,
> bakery basket, butter, preserves, coffee, hot tea, milk

**[Session IX:]{.underline} Immunological Processes In Aquatic
Organisms**

> **[Lester Khoo]{.underline}, moderator**

University of Pennsylvania, Kennett Square, PA

8:15 Anderson The Prophenoloxidase Activating System Of The Lobster 51

> *Homarus americanus*

8:30 Wiens Identification Of Tumor Necrosis Factor (TNF) And
TNF-Receptor 52

> Superfamily Members Expressed In Rainbow Trout (*Oncorhynchus mykiss*)

8:45 Burge Striped Bass (*Morone saxatilis*) *Nramp* Is Inducible *In
Vitro* After 53

> Exposure To LPS And *Mycobacterium marinum*

9:00 Dacanay The Response Of The Immunome Of Haddock To Vaccination 54

9:15 Pasnik Potency And Shelf-Life Of A *Streptococcus agalactiae*
Vaccine 55

> In Nile Tilapia (*Oreochromis niloticus*)

9:30 Xu Immune Responses Of Channel Catfish Against *Ichthyophthirius*
56

> m*ultifiliis* After Immunization With Live Theronts And Sonicated
> Trophonts

9:45 **Open Discussion**

10:00 **Morning Break**

**[Session X:]{.underline} Helminths of Fish**

> **[Alistair Dove]{.underline}, moderator**
>
> Cornell University, Stony Brook, NY

10:30 Dove *Bothriocephalus acheilognathi*: The Australian Experience 57

10:45 Mitchell Bolbophorus Infections In Catfish: Identification, Impact
And 58

> Treatment

11:00 Cartwright Preliminary Study On The Mitochondrial Genome Of The 59

> Tapeworm *Bothriocephalus acheilognathi*

11:15 Caira Cestodes Of Elasmobranches: Friends Or Foes 60

11:30 Sokolowski Histopathological Examination Of Wild American Eels,
*Anguilla* 61

> *rostrata,* Infected With *Anguillicola crassus*

11:45 Burreson The State Of Science Of Leech Parasites Of Fish 62

12:00 **Lunch**

Chicken Florentine Roulades stuffed with spinach, cream cheese and pine
nuts served over white and wild rice with grapes and champagne sauce,
tomato parmesan, fesh baked bread, chocolate amaretto mousse with
raspberry sauce, iced tea and coffee

**[Session XI:]{.underline} Chlamydial And Viral Pathogens**

> **[Paul Bowser]{.underline}, moderator**

Cornell University, Ithaca, NY

1:30 Draghi Molecular Characterization Of The Chlamydia-Like Bacterium
63 Associated With Epitheliocystis In Arctic Char (*Salvelinus alpinus*)

1:45 Bowser Prevalence Of Walleye Discrete Epidermal Hyperplasia And
Walleye 64

> Dermal Sarcoma By Age Class In Walleyes (*Sander vitreus*) From
>
> Oneida Lake, New York

2:00 Merrill Maine Strain II: A Case History Of The Multidisciplinary
Analysis 65

> Of An Emerging Infectious Salmon Anemia Virus Strain Variant

2:15 Giray Environmental Persistence Of Infectious Salmon Anemia Virus
66

2:30 Shivappa Critical Factors For Establishment Of Biosafety Level-3
Aquaria 67

2:45 **Open Discussion**

3:00 **Afternoon Break**

**[Session XII:]{.underline} Physical, Chemical And Nutritional Elements
Affecting Aquatic Animal Health**

> **Vicki S. Blazer, moderator**

National Fish Health Research Laboratory, Kearneysville, WV

3:30 Demers The Acute Effects Of A Handling Stressor On Select 68

> *Oreochromis aureus* Plasma Components

3:45 Pasnik Absence Of Intestinal Histologic Changes In Fingerling 69

> Channel Catfish (*Ictalurus punctatus*) Fed Raw Soybean Meal

4:00 Iwanowicz Histopathological And Physiological Evidence Of Thyroid
70

> DisruptionIn Common Carp (*Cyprinis carpio*) From The Middle
>
> And Lower Rio Grande River Basin

4:15 Darwish Copper Sulfate Target Animal Safety In Channel Catfish 71

4:30 **Open Discussion**

4:45 **Adjourn**

7:00 **Banquet and dance**

> **"The Hawaiian Luau"** includes tropical watermelon basket with fresh
> fruit, Polynesian carot salad, cole slaw with pineapple, Pacific conch
> chowder, broiled mahi-mahi with coconut cream sauce, Maui style sweet
> and sour chicken, honey suckling pig with condiments, island rice,
> green beans with water chestnuts, royal rum sweet potatoes, sweet corn
> squares, banana bread, lemon pie, island fruit tarts, coconut pie,
> coffee and iced tea
>
> **[Dancing]{.underline}** by Patrick Pake, DJ America, Inc.

### Friday, March 26

**[Optional Program]{.underline}: [Tumor Biology In Fish]{.underline}**

#### 

#### 

#### American Fisheris Society/Fish Health Section Continuing Education Class

### 

7:00 **Breakfast**

> Assorted juices, fresh fruit bowl, scrambled eggs, bacon, sausage,
> breakfast potatoes, grits, French toast with syrup, cold cereals,
> bakery basket, butter, preserves, coffee, hot tea, milk

8:30 **Class Begins**

**[Instructors:]{.underline}**

Vicki Blazer, National Fish Health Res. Laboratory, Kearneysville, WV

John Fournie, Environmental Protection Agency, Gulf Breeze, FL

> Paul Bowser, Cornell University, Ithaca, NY
>
> Jeffrey Wolf, Experimental Pathology Laboratories, Inc., Sterling, VA

10:00 **Morning Break**

12:00 **Lunch**

Roasted pork loin with apple brandy sauce, white and wild rice with
cranberries, green beans almondine, fresh baked breads, pumpkin pie,
iced tea and coffee

1:30 **Tumor Biology in Fish**, continues

3:00 **Afternoon Break**

4:30 **Class** **Adjourns**

**Dinner On Your Own**