**CURRICULUM VITAE**

**Yan Huang, M.D., Ph.D.**

**Citizenship**: USA

**Business Address**: Strom Thurmond Biomedical Research Center, Room
531

114 Doughty St.

Ralph H. Johnson VA Medical Center

Charleston, SC 29403

**Business telephone**: (843) 789-6824 (office)

**Fax**: (843) 876-5133

**E-mail**: huangyan@musc.edu.

## 

## EDUCATION

> 1977-1982 Shanghai Second Medical University, China M.D. Medicine
>
> 1986-1988 Univ. of Cincinnati, Cincinnati, Ohio M.S. Experimental
> Pathology
>
> 1988-1992 Univ. of Cincinnati, Cincinnati, Ohio Ph.D. Experimental
> Pathology

## POST-DOCTORAL CLINICAL TRAINING

1982-1986 Resident Internal Medicine Shanghai Sixth Hospital

## POST-DOCTORAL RESEARCH TRAINING

> 1992-1993 Postdoctoral Fellow (American Heart Association Fellowship),
> Dept. of Pathology, Univ. of Cincinnati, Cincinnati, Ohio
>
> 1993-1994 Postdoctoral Fellow, Division of G.I. Hormone, Dept. of
> Surgery Univ. of Cincinnati, Cincinnati, Ohio

## PROFESSIONAL APPOINTMENTS

1999-present VA Principal Investigator/Research Health Scientist,
Research Service, VA Medical Center, Charleston, SC

2006-present Associate Professor, Div. of Endocrinology, Diabetes, and
Medical Genetics, Dept. of Medicine, Medical University of South
Carolina, Charleston, SC

1995-2006 Assistant Professor, Div. of Endocrinology, Diabetes, and
Medical Genetics, Dept. of Medicine, Medical University of South
Carolina, Charleston, SC

### 1997-present Faculty member, College of Graduate Studies, Medical University of South Carolina, Charleston, SC 

### 

### PROFESSIONAL COMMITTEE ASSIGNMENT 

2005-present Committee Member, VA Biosafety Committee, Ralph H.

#  Johnson VA Medical Center, Charleston, SC 

2001-2004 Committee member, VA Research & Development Committee, Ralph
H.

Johnson VA Medical Center, Charleston, SC

# 

# **MEMBERSHIP**

2000-present Council member, Arteriosclerosis, Thrombosis, and Vascular
Biology

Council, American Heart Association

2000-present American Diabetes Association

2005- present American Physiological Society

## RESEARCH INTERESTS

1\. Pathogenesis of cardiovascular complications of diabetes, in
particular, the roles of matrix metalloproteinase, macrophage Fc gamma
receptor, peroxisome proliferator-activated receptors (PPARs), and
oxidation/glycation of lipoproteins;

2\. The role of matrix metalloproteinase in periodontal diseases in
diabetes;

3\. The role of dietary flavonoids in cardiovascular diseases.

**CURRENT RESEARCH GRANTS**

1\. VA Merit Review grant: "Diabetes and the stability of
atherosclerotic plaques", Principal Investigator, \$211,900/year,
4/1/2007-3/31/2010.

2\. NIH RO1 DE-16353: "Diabetes and periodontal gene expression",
Principal Investigator, \$213,000/year, 7/15/2005-6/30/2010.

# 3. Takeda Pharmaceuticals, Inc., pre-clinical grant: "The Effect of alogliptin on vascular inflammation and atherosclerosis", Principal investigator, \$144,760/year, 2/1/2009-1/31/2010.

## 

## PREVIOUS RESEARCH GRANTS

**I. [Previous research grants received as a PI]{.underline}**:

1\. Post-doctoral Research Fellowship awarded by the American Heart
Association: "Translational regulation of cholesterol esterase
expression in pancreatic AP42J cells" (Mentor: David Y. Hui, Ph.D.),
\$30,000, 7/1/1992-6/30/1993.

2\. Institution Research Grant, Medical University of South Carolina:
"Regulation of matrix metalloproteinase expression in endothelial
cells", Principal Investigator, \$12,000, 4/1/1998-3/31/1999.

3\. Atorvastatin Research Award, Parke-Davis and Pfizer: "Oxidized
LDL-activated expression of matrix metalloproteianse-1 and its role in
plaque vulnerability", Principal Investigator, \$50,000,
7/1/1999-6/30/2000.

4\. VA Merit Review Grant: "Regulation of matrix metalloproteinase-1
expression by oxidized LDL", Principal Investigator, \$96,000/year,
10/1/1999-9/30/2002.

5\. American Heart Association Grant-in-aid, Mid-Atlantic Affiliate:
"Signaling regulation of matrix metalloproteinase-1 expression by
co-crosslinking of Fc gamma receptor I and II", Principal Investigator,
\$50,000/year, 7/1/2000-6/30/2002.

6\. Takeda Pharmaceutical North America, Inc.: "The effect of
pioglitazone on the stability of advanced atherosclerotic plaques",
Principal Investigator, \$80,000/year, 3/1/2003-2/30/2005.

7\. Institutional Research Grant: "Diabetes and Periodontal Expression
of MMPs and cytokines", Principal Investigator, \$17,000 from
4/1/2003-3/31/2004.

8\. VA Merit Review Grant: "The effect of insulin-sensitizing
thiazolidinediones on the stability of atherosclerotic plaques",
Principal Investigator, \$150,000/year, 4/1/2003-3/31/2006.

**II. [Previous research grants received as a Co-PI]{.underline}**:

1\. NHLBI, NIH, R01-HL46815 : \"Macrophage Activation and Lipoprotein
Metabolism,\" Co-investigator (PI: Lopes-Virella M.F., MD, PhD),
\$707,233 total, 12/1/98-11/30/01.

2\. American Heart Association Grant-in-aid, AHA National Center:
"Molecular mechanisms of LDL-receptor upregulation in cholesterol-laden
cells." Co-investigator (PI: Lopes-Virella M.F. MD, PhD), \$150,000
total, 1/1/98-12/31/2001.

3\. NIH, Program project: "Marker and Mechanism of Vascular Disease in
Diabetes". Co-investigator (Principal Investigator: Lopes-Virella, M.F.,
MD, PhD). 10/1/1995-9/30/2006.

**RESEARCH Trainees**

(Serving as mentor for the following trainees)

+-----------------------+-----------------------+----------------------+
| ###### Name           | [Status]{.underline}  | ###                  |
|                       |                       | ### Present Position |
+-----------------------+-----------------------+----------------------+
| Minfu Xu, M.D., Ph.D. | Post-doctoral fellow  | Post-doctoral fellow |
|                       |                       |                      |
|                       | 1999-2000             | Department of        |
|                       |                       | Psychiatrics         |
+-----------------------+-----------------------+----------------------+
| Fan Fan, M.D.         | Post-doctoral fellow  | Post-doctoral fellow |
|                       |                       |                      |
|                       | 1999-2000             | Dept. of             |
|                       |                       | Neuroscience         |
+-----------------------+-----------------------+----------------------+
| Daming Tang, M.D.,    | Post-doctoral fellow  | Post-doctoral fellow |
| Ph.D.                 |                       |                      |
|                       | 2000-2001             | University of Texas  |
+-----------------------+-----------------------+----------------------+
| Alejandro Maldonado,  | Post-doctoral fellow  | Physician, Clinical  |
| M.D.                  |                       | Center "Dr. Marcial  |
|                       | 2002-2003             | Ríos", Mérida,       |
|                       |                       | Venezuela            |
+-----------------------+-----------------------+----------------------+
| Alena Nareika, M.D.,  | Post-doctoral fellow  |                      |
| Ph.D.                 |                       |                      |
|                       | 2003-present          |                      |
+-----------------------+-----------------------+----------------------+
| Lauren Ball, Ph.D.    | Oral Health COBRE     | Assistant Professor  |
|                       | Principal             | of Pharmacology,     |
|                       | Investigator          | MUSC                 |
|                       |                       |                      |
|                       | July, 2007-present    |                      |
+-----------------------+-----------------------+----------------------+
| Chris Cole, DDS, MS   | Fellow, Dept of       | Private Practice,    |
|                       | Stomatology, College  | State of Tennessee   |
|                       | of Dental Med, MUSC   |                      |
|                       |                       |                      |
|                       | July, 2007-present    |                      |
+-----------------------+-----------------------+----------------------+

**RESEARCH Trainees**

(Serving as co-mentor with Dr. Lopes-Virella for the following trainees)

+-----------------------+-----------------------+----------------------+
| ###### Name           | [Status]{.underline}  | ###                  |
|                       |                       | ### Present Position |
+-----------------------+-----------------------+----------------------+
| Marina Mironova, M.D. | Post-doctoral fellow  | East Carolina        |
|                       |                       | Univ.-Int Med        |
|                       | 1994-2000             | Residency            |
+-----------------------+-----------------------+----------------------+
| Akira Takei, M.D.     | Post-doctoral fellow  | Assistant Professor  |
|                       |                       | Kyushu University    |
|                       | 1996-98               | Fukuoka, Japan       |
+-----------------------+-----------------------+----------------------+
| Sinikka Koskinen, M.D | Post-doctoral fellow  | Staff Physician      |
|                       |                       |                      |
|                       | 1996-1999             | University of        |
|                       |                       | Helsinki             |
|                       |                       |                      |
|                       |                       | Hospital             |
+-----------------------+-----------------------+----------------------+
| Sumita Bandyopadhyay, | Post-doctoral fellow  | Post-doctoral        |
| Ph.D.                 |                       | fellow, MUSC         |
|                       | 1998-2000             |                      |
+-----------------------+-----------------------+----------------------+
| Mitsunori Masakado,   | Post-doctoral fellow  | 3^rd^ Department of  |
| MD                    |                       | Internal Medicine    |
|                       | 2000-2003             |                      |
|                       |                       | Kyushu University    |
|                       |                       | Fukuoka, Japan       |
+-----------------------+-----------------------+----------------------+

# 

# **Graduate Student Advisory Committee**

Malabika Ghosh, Ph.D., 1997.

# **Research Advisor for Undergraduate Students (Summer Projects)**

Aprill Dawson, Xavier University, New Orleans, Louisiana, 2001,

Lauren Thompson, Hampton University, SC, 2001,

Jocelyn Myers, Johnson C. Smith Unviersity, NC, 2002,

Cecilia Zhang, Government School, SC, 2003,

Takeyla Williams, College of Charleston, SC, 2003.

Veronica Jarido, University of Pittsburgh, 2004.

Veronica Jarido, University of Pittsburgh, 2005.

Shawan Hughes, South Carolina State University, 2006

Crystal Brown, Clemson University, 2007

Sana Li, Columbia College, 2008

Crystal Brown, Clemson University, 2008

**TEACHING (POST-GRADUATE COURSE)**

2002 Spring, Introduction of Epidemiology (Course Director: Deyi Zheng,
MD, PhD), Department of Biometry and Epidemiology, Medical University of
South Carolina

2005-present Introduction of Molecular Endocrinology, a lecture series
for clinical and research fellows, Division of Endocrinology, Diabetes
and Medical Genetics, Department of Medicine, Medical University of
South Carolina

#### 

#### Invited Presentations

**"** Oxidized LDL stimulates matrix metalloproteinase-1 expression in
endothelium and macrophages", 7th Southeast Lipid Conference, Atlanta,
September, 1998.

**"** Fc gamma receptor signaling and metalloproteinase-1 expression in
human U937 histiocytes", 8th Southeast Lipid Conference, Atlanta,
September, 1999.

"Regulation of matrix metalloproteinase-1 in vascular endothelial
cells", Vancouver, Canada, sponsored by the Parke-Davis and Pfizer,
2000.

"Matrix metalloproteinases and plaque rupture", Shanghai Ninth Hospital,
Shanghai, China, sponsored by Shanghai Second Medical University, March
2001.

"Regulation of matrix metalloproteinase-1 expression by inflammation
agonists and antagonists", University of Oklahoma College of Medicine,
Oklahoma City, Oklahoma, November 2003.

"Regulation of MMP expression by inflammation stimulators and
inhibitors", Southeast Lipid Research Conference, Callaway Garden,
Georgia, September 2005.

## PUBLICATIONS

1\. **Huang Y**, Hui DY. Metabolic fate of pancreas-derived cholesterol
esterase in intestine. An in vitro study using Caco-2 cells. J. Lipid
Res. 31:2029-2037, 1990.

2\. **Huang Y**, Hui DY. Cholesterol esterase biosynthesis in rat
pancreatic AR42J cells. Post-transcriptional activation by gastric
hormones. J. Biol. Chem. 266:6720-6725, 1991.

3\. **Huang Y**, Hui DY. Synergistic effects of bombesin and
cholecystokinin on cholesterol esterase biosynthesis and secretion by
AR42J cells. Arch. Biochem. Biophys. 310:54-59, 1993.

4\. **Huang Y**, Hui DY. Increased cholesterol esterase level by
cholesterol loading of rat pancreatoma cells. Biochim Biophys Acta
1214:317-322, 1994.

5\. Balasubramaniam A, Sheriff S, Johnson ME, Prabhakaran M, **Huang
Y**, Fischer JE, Chance WT. \[D-Trp32\] Neuropeptide Y: a competitive
antagonist of NPY in rat hypothalamus. J. Med. Chem. 37:811-815, 1994.

6\. Balasubramaniam A, Ujhelyi M, Borchers M, **Huang Y**, Zhai W, Zhou
Y, Johnson M, Sheriff S, Fischer JE. Antagonistic properties of
centrally truncated analogs of \[D-Trp32\] NPY. J. Med. Chem.
39:1142-1147, 1996.

7\. Li F, **Huang Y**, Hui DY. Bile salt-stimulated cholesterol esterase
increases uptake of high density lipoprotein-associated cholesteryl
esters by HepG2 cells. Biochemistry, 35:6657-6663, 1996.

8\. **Huang Y**, Fischer JE, Balasubramaniam A. Amylin mobilizes
intracellular calcium through exclusive receptors on rat pancreatic
AR42J cell line. Peptides 17:497-502, 1996.

9\. Balasubramaniam A, Zhai W, Tao Z, **Huang Y**, Fischer JE, Eden P,
Taylor JE, Kar L, Samarasinghe SD, Johnson ME. Synthesis, structure and
antagonistic properties of Des-Asn29\[D-Trp28,32\]NPY(27-36). Peptides
17:1113-1118, 1996.

10\. **Huang Y**, Ghosh M, Lopes-Virella MF. Transcriptional and
post-transcriptional regulation of LDL receptor gene expression in
PMA-treated THP-1 cells by LDL-containing immune complexes. J. Lipid
Research 38:110-120, 1997.

11\. **Huang Y**, Ayad J, Koskinen S, Takei A, Lopes-Virella MF.
Oxidized LDL-containing immune complexes induce Fc gamma receptor
I-mediated mitogen-activated protein kinase activation in THP-1
macrophages. Arteriosclerosis, Thrombosis, and Vascular Biology,
19:1600-1607, 1999.

12\. **Huang Y**, Mironova M, Lopes-Virella MF. Oxidized LDL stimulates
matrix metalloproteinase-1 expression in human vascular endothelial
cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 19:2640-2647,
1999.

13\. **Huang Y**, Fleming AJ, Wu S, Virella G, Lopes-Virella MF. Fc
gamma receptor cross-linking by immune complexes induces matrix
metalloproteinase-1 in U937 cells via mitogen-activated protein kinase
cascade. Arteriosclerosis, Thrombosis, and Vascular Biology,
20:2533-2538, 2000.

14\. Klein R, Ascencao JL, Mironova M, **Huang Y**, Lopes-Virella MF.
Effect of inflammatory cytokines on the metabolism of low density
lipoproteins by human vascular endothelial cells. Metabolism 50:99-106,
2001.

15\. Takei A, **Huang Y**, Lopes-Virella MF. Intercellular adhesion
molecule-1 (ICAM-1) expression induced by oxLDL on human umbilical vein
endothelial cells (HUVEC) depends on the stage of LDL oxidation.
Atherosclerosis 154:79-86, 2001.

16\. **Huang Y**, Song L, Wu S, Fan F, Lopes-Virella MF. Oxidized LDL
differentially regulates MMP-1 and TIMP-1 expression in human vascular
endothelial cells. Atherosclerosis 156:119-125, 2001.

17\. Song L, Lopes-Virella MF, and **Huang Y**. Quercetin inhibits
oxidized LDL-stimulated matrix metalloproteinase-1 expression in
endothelium by blocking extracellular signal regulated protein kinase.
Archives of Biochemistry and Biophysics 391:72-78, 2001.

18\. Anderson F, Xu M, Game BA, Atchley D, Lopes-Virella MF, **Huang
Y**. IFN-γ pre-treatment augments immune complex-induced matrix
metalloproteinase-1 expression in U937 histiocytes. Clinical Immunology
102:200-207, 2002.

19\. **Huang Y**, Fu Y, Bandyopadhyay S, Virella G, Lopes-Virella MF.
LDL Immune Complexes Stimulate Low Density Lipoprotein Receptor
Expression in U937 Histiocytes via Extracellular Signal-Regulated Kinase
and AP-1. Journal of Lipid Research 44:1315-1321, 2003.

20\. Game BA, Tang D, Minfu Xu, Lopes-Virella MF, **Huang Y**.
Regulation of MMP-1 expression in vascular endothelial cells by
insulin-sensitizing thiazolidinediones. Atherosclerosis 169:235-243,
2003.

21\. Maldonado A, Game BA, Song L, **Huang Y**. Upregulation of matrix
metalloproteinase-1 expression in U937 cells by LDL-containing immune
complexes requires the AP-1 and Ets Motifs in the distal and the
proximal promoter region. Immunology 109:572-579, 2003.

22\. Williams TN, Zhang C, Game BA, He L, **Huang Y**. C-reactive
protein stimulates MMP-1 expression in U937 histiocytes through FcγRII
and ERK pathway. Arteriosclerosis, Thrombosis, and Vascular Biology,
24:61-66, 2004.

23\. Maldonado A, He L, Game BA, Nareika A, Sanders JJ, London SD,
Lopes-Virella MF, Huang Y. Pre-Exposure to High Glucose Augments
Lipopolysaccharide-Stimulated Matrix Metalloproteinase-1 Expression by
Human U937 Histiocytes. Journal of Periodontal Research 39, 415-423,
2004.

24\. Game BA, Maldonado A, Huang Y. Pioglitazone inhibits MMP-1
expression in vascular smooth muscle cells through a mitogen-activated
protein kinase-independent mechanism. Atherosclerosis 178:249-256, 2005.

25\. Kraml PJ, Klein RL, Huang Y, Nareika A, Lopes-Virella MF. Iron
Loading Increases Cholesterol Accumulation and Macrophage Scavenger
Receptor-I Expression in THP-I Mononuclear Phagocytes. Metabolism
54:453-459, 2005.

26\. Song W, Barth JL, Lu K, Yu Y, Huang Y, Gittinger CK, Argraves WS,
Lyons TJ. Effects of oxidized and glycated LDL on gene expression in
human retinal capillary pericytes, Investigative Ophthalmology & Visual
Science, 46:2974-2982, 2005.

27\. Song W, Barth JL, Lu K, Yu Y, Huang Y, Gittinger CK, Argraves WS,
Lyons TJ. Effects of modified low-density lipoproteins on human retinal
pericyte survival. Ann NY Acad Sci. 1043:390-395, 2005.

28\. Nareika A, He L, Game BA, Slate EH, Sanders JJ, London SD,
Lopes-Virella MF, **Huang Y**. Sodium lactate increases LPS-stimulated
MMP and cytokine expression in U937 histiocytes by enhancing AP-1 and
NFkB transcriptional activities. American Journal of Physiology
(Endocrinology and Metabolism) 289:E534-E542, 2005.

29\. He L, Game BA, Nareika A, Garvey WT, Huang Y. Administration of
Pioglitazone in Low-Density Lipoprotein Receptor-Deficient Mice Inhibits
Lesion Progression and MMP Expression in Advanced Atherosclerotic
Plaques. Journal of Cardiovascular Pharmacology 48:212-222, 2006.

30\. Nareika A, Maldonado A, He L, Game BA, Slate EH, Sanders JJ, London
SD, Lopes-Virella MF, **Huang Y**. High Glucose-Boosted Inflammatory
Responses to Lipopolysaccharide Are Suppressed by Statin. Journal of
Periodontal Research 42:31-38, 2006.

31\. Game BA, He L, Jarido V, Nareika A, Jaffa AA, Lopes-Virella MF,
**Huang Y**. Pioglitazone inhibits connective tissue growth factor
expression in advanced atherosclerotic plaques in low-density
lipoprotein receptor-deficient mice. Atherosclerosis 192:85-91, 2007.

32\. Barth JL, Yu Y, Song W, Lu K, Dashti A, **Huang Y**, Argraves WS,
Lyons TJ. Oxidised, glycated LDL selectively influences tissue inhibitor
of metalloproteinase-3 gene expression and protein production in human
retinal capillary pericytes. Diabetologia 50:2200-2208, 2007.

33\. Cole CM, Sundararaj KP, Leite RS, Nareika A, Slate EH, Sanders JJ,
Lopes-Virella MF, **Huang Y**.

> A trend of increase in periodontal IL-6 expression across patients
> with neither diabetes nor periodontal disease, patients with
> periodontal disease alone, and patients with both diseases. Journal of
> Periodontal Research 43:717-722, 2008.

34\. Nareika A, Im Y, Game BA, Slate EH, Sanders JJ, London SD,
Lopes-Virella MF, **Huang Y**, High glucose enhances
lipopolysaccharide-stimulated CD14 expression in U937 mononuclear cells
by increasing NFκB and AP-1 activities, Journal of Endocrinology
196:45-55, 2008.

35\. Sundararaj KP, Samuvel DJ, Li Y, Nareika A, Slate EH, Sanders JJ,
Lopes-Virella MF, **Huang Y**. Simvastatin Suppresses LPS-Induced MMP-1
Expression in U937 Mononuclear Cells by Inhibiting Protein
Isoprenylation-mediated ERK Activation, Journal of Leukocyte
Biology,84:1120-1129, 2008.

36\. Nareika A, Sundararaj KP, Im Y, Game BA, Lopes-Virella MF, **Huang
Y**. High Glucose and Interferon Gamma Synergistically Stimulate MMP-1
Expression in U937 Macrophages by Increasing Transcription Factor STAT1
Activity. Atherosclerosis, in press, 2009.

37\. Samuvel DJ, Sundararaj KP, Nareika A, Lopes-Virella MF, **Huang
Y**. Lactate Boosts TLR4 Signaling and NFκB Pathway-Mediated Gene
Transcription in Macrophages via Monocarboxylate Transporters and MD-2
Upregulation. Journal of Immunology, in press, 2009.

## PRESENTATIONS AND ABSTRACTS

Hui DY, **Huang Y**. Cholesterol esterase mediated uptake of high
density lipoprotein-associated cholesteryl esters by HepG2 cells.
Arteriosclerosis 9:711a, 1989, presented to the 62nd National Meeting of
the American Heart Association.

**Huang Y**, Hui DY. Post-transcriptional regulation of cholesterol
esterase gene expression. Arteriosclerosis 10:786a, 1990, Presented to
the 63rd National Meeting of the American Heart Association.

**Huang Y**, Hui DY. Cholecystokinin regulation of cholesterol esterase
biosynthesis in a rat pancreatic tumor cell line. FASEB J. 4:A1747,
1990.

Brodt-Epply J, **Huang Y**, Hui DY. Protein kinase C-dependent
cholecystokinin activation of pancreatic cholesterol esterase
biosynthesis. FASEB J. 8:A1309, 1994, Presented to the 67th National
Meeting of the American Heart Association.

**Huang Y**, Fischer JE, Balasubramaniam A. Des-AA7-24\[D-Ala5, Aoc6,
D-Trp32\] NPY & Des- Asn29\[D-Trp28,32\]NPY (27-36): Y-1 receptor
antagonists. 76th Endocrine Society Annual Meeting, Program & Abstracts,
p604, 1994, Presented to the 76th National Meeting of the Endocrine
Society.

Ghosh M, **Huang Y** and Lopes-Virella MF. Modified LDL-immune complexes
upregulates macrophage scavenger receptors in THP-1 cells. American
Heart Association 69th Scientific Sessions, New Orleans, LA, November
10-13, 1996.

**Huang Y**, Ghosh M and Lopes-Virella MF: Transcriptional and
post-transcriptional regulation of the LDL receptor gene in THP-1 cells
stimulated by LDL-IC. American Heart Association 69th Scientific
Sessions, New Orleans, LA, November 10-13, 1996.

**Huang Y**, Ghosh M and Lopes-Virella MF: Massive increase in ACAT
activity induced by Fc receptor mediated uptake of LDL-IC. American
Heart Association 69th Scientific Sessions, New Orleans, LA, November
10-13, 1996.

**Huang Y**, Nussbaum N and Lopes-Virella MF: Oxidized LDL-containing
immune complexes induce mitogen-activated protein kinase phosphorylation
in human macrophages. J. Invest. Med. 46:232A, 1998. Presented at the
Biomedicine\'98 National Meeting, Washington, DC, May 1-3, 1998.

Takei A, **Huang Y** and Lopes-Virella MF: Intercellular adhesion
molecule-1 (ICAM-1) expression induced by oxLDL on human umbilical vein
endothelial cells (HUVEC) depends on the stage of LDL oxidation.
Presented at the American Diabetes Association Annual Meeting, Chicago,
IL, 1998.

**Huang Y** and Maria Lopes-Virella. Oxidized LDL stimulates matrix
metalloproteinase-1 expression in endothelium and macrophages. Presented
at the 7th Southeast Lipid Conference, Atlanta, September 10-13, 1998.

**Huang Y**, Mironova M and Lopes-Virella MF: Oxidized LDL stimulates
matrix metalloproteinase-1 expression in human vascular endothelial
cells. Circulation (supplement), 17:I-813, 1998. presented at the 71st
American Heart Association Scientific Sessions, Dallas, November 8-11,
1998.

Lopes-Virella MF, Takei A, Crawford A, and **Huang Y**. Advanced
glycation end-products-LDL stimulates adhesion molecule expression by
human aortic endothelial cells. Diabetes (supplement 1) 48:A31, 1999.
Presented at the 59th American Diabetes Association Scientific Sessions,
June 19-22, 1999.

**Huang Y** and Lopes-Virella MF. Fc gamma receptor signaling and
metalloproteinase-1 expression in human U937 histiocytes. Presented at
the 8th Southeast Lipid Conference, Atlanta, September 9-12, 1999.

**Huang Y**, Shan Wu, Fleming AJ, Virella G, and Lopes-Virella, MF.
LDL-containing immune complexes induce macrophage metalloproteinase-1
expression via activation of a Fc gamma receptor I-linked
mitogen-activated protein kinase pathway. Circulation (supplement),
18:I-252, 1999. Presentation at the 72nd American Heart Association
Scientific Sessions in Atlanta, Georgia, November 7-10, 1999.

Christov SM, Klein RL, **Huang Y**, Best JD, Lyons TJ, Jenkins AJ.
Comparison of native versus modified LDL and Lp(a) on total and ester
cholesterol accumulation by macrophages derived from THP-1 monocytes.
Presented at the First Conference on Arteriosclerosis, Thrombosis, and
Vascular Biology. Denver, Colorado, May 20-22, 2000.

Xu M, Game BA, Lopes-Virella MF, **Huang Y**. Insulin sensitizer
troglitazone stimulates matrix metalloproteinase-1 expression in human
vascular endothelial cells. Presented at the American Diabetes
Association 61^st^ Scientific Sessions, Philadelphia, PA, 2001. Diabetes
50 (Supplement 2): A170, 2001.

Game BA, Lopes-Virella MF, **Huang Y**. PPAR gamma ligand 9-HODE and
troglitazone stimulate matrix metalloproteinase-1 expression in vascular
endothelial cells via extracellular signal-regulated kinase. Presented
at the American Heart Association Arteriosclerosis, Thrombosis, and
Vascular Biology Conference, Salt Lake City, 2002.

Game BA, Tang D, Lopes-Virella MF, **Huang Y**. Inhibition of total
protein synthesis by troglitazone through eIF4E leads to blocking of
MMP-1 translation in endothelial cells despite a marked increase in
MMP-1 mRNA. Presented at the American Diabetes Association 62^nd^ Annual
Meeting, San Francisco, CA, 2002.

Song W, **Huang Y**, Barth JL, Jenkins AJ, Jaffa AA, Gittinger CK,
Argraves WS, Lyons WT. Effects of modified LDL on gene expression by
human retinal capillary pericytes. Presented at the American Diabetes
Association 62^nd^ Annual Meeting, San Francisco, CA, 2002.

Game BA, Maldonado A, Tang D, **Huang Y**. Pioglitazone inhibits
oxidized LDL-stimulated matrix metalloproteinase-1 expression in human
vascular smooth muscle cells. Presented at the South Carolina Diabetes
Conference, North Charleston Convention Center, South Carolina,
September 2002.

Game BA, Maldonado Poyato A, Lopes-Virella MF, **Huang Y**. Crosslinking
of Fc gamma receptor I and II synergistically stimulates extracellular
signal-regulated kinase that induces AP-1 and Ets-mediated matrix
metalloproteinase-1 expression in U937 histiocytes. Presented at the
American Heart Association 75^th^ Scientific Sessions, Chicago, IL,
November 17-20, 2002.

Game BA, Maldonado A, **Huang Y**. Pioglitazone inhibits oxidized
LDL-stimulated matrix metalloproteinase-1 expression in human vascular
smooth muscle cells through a mitogen-activated protein
kinase-independent mechanism. Diabetes 2003;52:A20. Presented at the
American Diabetes Association 63^rd^ Scientific Sessions, New Orleans,
LA, 2003.

He L, Maldonado A, **Huang, Y**. Pre-Exposure of U937 Histiocytes to
High Glucose Augments LPS-Stimulated Cytokine and Chemokine Expression.
American Diabetes Association 64^th^ Scientific Sessions 2084-PO,
Orlando, FL, 2004.

Song W, **Huang Y**, Lu K, Barth JL, Argraves S, Lyons T.
Heavily-Oxidized-Glycated LDL Inhibits TIMP-3 Expression in Human
Retinal Capillary Pericytes. American Diabetes Association 64^th^
Scientific Sessions 898-P, Orlando, FL, 2004.

Lu K, Song W, **Huang Y**, Barth J, Argraves S, Lyons TJ. Effect of
native LDL and modified LDL on mRNA expression of genes involved in
lipid metabolism in human retinal pericytes. American Diabetes
Association 64^th^ Scientific Sessions 1084-P, Orlando, FL, 2004.

He L, Game BA, Garvey WT, **Huang Y**. Administration of Pioglitazone to
LDL Receptor-Deficient Mice Inhibits Progression of Intimal Lesions and
Expression of Matrix Metalloproteinases and Adhesion Molecules. American
Diabetes Association 65^th^ Scientific Sessions 738-P, San Diego, CA,
2005. Abstract published in Diabetes 54 (Supplement 1), A182, 2005.

Game BA, He L, Jarido V, Jaffa AA, Lopes-Virella MF, **Huang Y**.
Pioglitazone Inhibits Connective Tissue Growth Factor Expression during
the Progression of Aortic Atherosclerotic Plaques in LDL
Receptor-Deficient Mice. American Diabetes Association 65^th^ Scientific
Sessions 750-P, San Diego, CA, 2005. Abstract published in Diabetes 54
(Supplement 1), A184, 2005.

Nareika A, He L, Game BA, Sanders JJ, London SD, Lopes-Virella MF,
**Huang Y**. Sodium Lactate and Lipopolysaccharide Exert Synergistic
Effect on MMP and Cytokine Expression in U937 Histiocytes through NFκB
and MAPK Pathways. American Diabetes Association 65^th^ Scientific
Sessions 1287-P, San Diego, CA, 2005. Abstract published in Diabetes 54
(Supplement 1), A313, 2005.

Nareika A, Maldonado A, He L, Game BA, Slate EH, Sanders JJ, London SD,
Lopes-Virella MF, **Huang Y**. High Glucose-Boosted Innate Inflammatory
Responses to Lipopolysaccharide Are Suppressed by Statin, Presented at
American Diabetes Association 66th Scientific Session, Washington, DC,
June 9-13, 2006. Abstract published in Diabetes 55 (Supplement 1), A160,
2006.

Nareika A, Im Y, Game BA, Slate EH, Sanders JJ, London SD, Lopes-Virella
MF, **Huang Y**. High Glucose Enhances Lipopolysaccharide-Induced CD14
Expression in U937 Histiocytes. Presented at American Diabetes
Association 66th Scientific Session, Washington, DC, June 9-13, 2006.
Abstract published in Diabetes 55 (Supplement 1), A171, 2006.

Nareika A, Game BA, Sanders JJ, Lopes-Virella MF, **Huang Y**. High
glucose augments LPS-stimulated MMP-1 expression in human
monocyte-derived macrophages. American Diabetes Association Scientific
Sessions, March 21-24, 2007, New Orleans, LA.

Nareika A, Game BA, Sanders JJ, London SD, Lopes-Virella MF, **Huang
Y**. A new method to quantify MMP-8 in oral rinse samples. American
Diabetes Association Scientific Sessions, March 21-24, 2007, New
Orleans, LA.

Nareika A, Game BA, Lopes-Virella MF, **Huang Y**. High glucose augments
IFN gamma-stimulated matrix metalloproteinase-1 expression in U937
macrophages by enhancing STAT-1 activity. American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology Annual Conference,
April 19-21, 2007, Chicago, IL.

Christine M. Cole, Kamala P. Sundararaj, Renata S. Leite, Alena Nareika,
Elizabeth H. Slate, John J. Sanders, Maria F. Lopes-Virella, Yan Huang.
Periodontal IL-6 Expression in Diabetic and Nondiabetic Patients.
American Association of Dental Research Annual Meeting, Dallas, TX,
April 2-5, 2008.

Sundararaj KP, Li Y, Samuvel DJ, Nareika A, Lopes-Virella MF, **Huang
Y.** Simvastatin Suppresses LPS-Induced MMP-1 Expression in U937
Mononuclear Cells by Inhibiting Ras and Rac Protein
Isoprenylation-mediated ERK Activation. Accepted for presentation at
American Heart Association Atherosclerosis, Thrombosis and Vascular
Biology annual meeting in Atlanta, Georgia, April 16-18, 2008.

Sundararaj KP, Samuvel DJ, Li Y, Slate EH, Sanders JJ, Lopes-Virella MF,
**Huang Y.** High glucose enhances fibroblast-mononuclear cell
co-culture-boosted MMP-1 expression. Accepted for presentation at 68^th^
American Diabetes Association Scientific Sessions in San Francisco, CA,
June 6-10, 2008.