**[Presenters and Titles]{.smallcaps}**

**[5^th^ International Symposium on the Interface]{.smallcaps}**

**[Between Analytical Chemistry and Microbiology]{.smallcaps}**

Sunday, April 18, 2004 6:00-8:00 p.m. Registration, Courtyard Marriott
Hotel Lobby

Monday, April 19, 2004

PNNL Auditorium Lobby, Richland, Washington

+--------------------------+-----------------------------+------------+
| **Presenter**            | **Title of Abstract**       | **Time**   |
+--------------------------+-----------------------------+------------+
| Complementary Bus to     |                             | 7:30 a.m.  |
| PNNL Campus from         |                             |            |
| Courtyard Marriott Lobby |                             | 7:45-8:30  |
|                          |                             | a.m.       |
| Registration PNNL        |                             |            |
| Auditorium Lobby         |                             |            |
+--------------------------+-----------------------------+------------+
|                          | **INTRODUCTIONS/WELCOME**   | 8:30 a.m.  |
+--------------------------+-----------------------------+------------+
| **ANALYTICAL METHODS     |                             |            |
| DEVELOPMENT SESSION,     |                             |            |
| Part I**                 |                             |            |
+--------------------------+-----------------------------+------------+
| Richard D Smith          | Advanced Technologies for   | 8:50-9:40  |
|                          | Comprehensive and           | a.m.       |
| Pacific Northwest        | Quantitative Microbial      |            |
| National Laboratory      | Proteomics                  |            |
+--------------------------+-----------------------------+------------+
| Darrell Chandler         | Functional Protein Arrays   | 9:45-10:05 |
|                          | at Argonne National         | a.m.       |
| Argonne National         | Laboratory                  |            |
| Laboratory               |                             |            |
+--------------------------+-----------------------------+------------+
| **BREAK**                |                             | 1          |
|                          |                             | 0:10-10:25 |
|                          |                             | a.m.       |
+--------------------------+-----------------------------+------------+
| Paul Majors              | NMR Methods for *in-situ*   | 1          |
|                          | Biofilm Metabolism and      | 0:25-10:45 |
| Pacific Northwest        | Transport Studies           | a.m.       |
| National Laboratory      |                             |            |
+--------------------------+-----------------------------+------------+
| Robert J Cotter          | Development of Compact Mass | 1          |
|                          | Spectrometers for Detection | 0:50-11:10 |
| Johns Hopkins University | of Biomarkers               | a.m.       |
| School of Medicine       |                             |            |
+--------------------------+-----------------------------+------------+
| Julie A Stenken          | Microdialysis Sampling as   | 1          |
|                          | an Enabling Technology for  | 1:15-11:35 |
| Rensselaer Polytechnic   | Monitoring Microbiological  | a.m.       |
| Institute                | Chemical Markers *in vivo*  |            |
+--------------------------+-----------------------------+------------+
| TBD                      | EMSL brief                  | 11:40      |
|                          |                             |            |
|                          | LUNCH EMSL 1077             | 12:00 p.m. |
|                          |                             |            |
|                          | EMSL LAB TOURS DURING LUNCH |            |
+--------------------------+-----------------------------+------------+
| **MICROBIAL CELL         |                             |            |
| COMPONENTS SESSION**     |                             |            |
+--------------------------+-----------------------------+------------+
| Günter Allmaier          | MALDI MS/MS and MS/MS/MS of | 1:30-2:10  |
|                          | Lipids, Lipases and         | p.m.       |
| Vienna University of     | Peptidoglycan Fragments     |            |
| Technology               |                             |            |
+--------------------------+-----------------------------+------------+
| Rachel Ogorzalek Loo     | Classical and Virtual 2-D   | 2:15-2:35  |
|                          | Gel Electrophoresis of      | p.m.       |
| University of California | *Methanosarcina             |            |
| at Los Angeles           | acetivorans*                |            |
+--------------------------+-----------------------------+------------+
| Judy A Shoemaker         | Identification of Allergens | 2:40-3:00  |
|                          | from *Metarhizium           | p.m.       |
| U.S. Environmental       | Anisopliae* Using Mass      |            |
| Protection Agency        | Spectrometry                |            |
+--------------------------+-----------------------------+------------+
| **BREAK**                |                             | 3:05-3:20  |
|                          |                             | p.m.       |
+--------------------------+-----------------------------+------------+
| Yuri Gorby               | Controlled Cultivation:     | 3:20-3:40  |
|                          | Strategies for Improving    | p.m.       |
| Pacific Northwest        | Our Understanding of the    |            |
| National Laboratory      | Biology Behind              |            |
|                          | Bioremediation              |            |
+--------------------------+-----------------------------+------------+
|                          |                             |            |
+--------------------------+-----------------------------+------------+
| Charles E Turick         | Surface Analyses of         | 3:45-4:05  |
|                          | Dissimilatory Metal         | p.m.       |
| Westinghouse Savannah    | Reducing Bacteria           |            |
| River Site               |                             |            |
+--------------------------+-----------------------------+------------+
| **Bus Return to Hotel**  |                             | 4:15 p.m.  |
|                          |                             |            |
| **Poster Session Set     |                             | 5:00-6:15  |
| up**                     |                             | p.m.       |
|                          |                             |            |
| **Poster Session/Wine    |                             | 6:30-8:30  |
| Tasting** Courtyard      |                             | p.m.       |
| Marriott Hotel           |                             |            |
+--------------------------+-----------------------------+------------+

**[Presenters and Titles]{.smallcaps}**

**[5^th^ International Symposium on the Interface]{.smallcaps}**

**[Between Analytical Chemistry and Microbiology]{.smallcaps}**

Tuesday, April 20, 2004

Courtyard Marriott Meeting Room, Richland, Washington

+--------------------------+-----------------------------+------------+
| **Presenter**            | **Title of Abstract**       | **Time**   |
+--------------------------+-----------------------------+------------+
| **HOMELAND               |                             |            |
| SECURITY/BIODEFENSE      |                             |            |
| SESSION, Part I**        |                             |            |
+--------------------------+-----------------------------+------------+
| Alvin Fox                | PCR and Protein Markers for | 8:30-9:20  |
|                          | Mass Spectrometry-Based     | a.m.       |
| University South         | Biodetection                |            |
| Carolina                 |                             |            |
+--------------------------+-----------------------------+------------+
| Catherine Fenselau       | Rapid Characterization of   | 9:25-9:45  |
|                          | Microorganisms in Mixtures  | a.m.       |
| University of Maryland   | by MALDI Mass Spectrometry  |            |
|                          | and Bioinformatics          |            |
+--------------------------+-----------------------------+------------+
| Vladimir M Doroshenko    | Differentiation of Various  | 9:50-10:10 |
|                          | Bacilli Using Atmospheric   | a.m.       |
| MassTech                 | Pressure MALDI Mass         |            |
|                          | Spectrometry                |            |
+--------------------------+-----------------------------+------------+
| BREAK                    |                             | 1          |
|                          |                             | 0:15-10:30 |
|                          |                             | a.m.       |
+--------------------------+-----------------------------+------------+
| Steven A Hofstadler      | TIGER: A PCR-Mass           | 1          |
|                          | Spectrometry-Based Approach | 0:30-10:50 |
| Ibis Therapeutics        | for the Detection and       | a.m.       |
|                          | Characterization of         |            |
|                          | Biothreats and Emerging     |            |
|                          | Infectious Diseases         |            |
+--------------------------+-----------------------------+------------+
| Kent J Voorhees          | Rapid Detection of Bacteria | 1          |
|                          | Using Phage Amplification   | 0:55-11:15 |
| Colorado School of Mines | with Immunodetection        | a.m.       |
|                          | Devices                     |            |
+--------------------------+-----------------------------+------------+
| James M Robertson        | The Microbial Genetics and  | 1          |
|                          | Forensics Initiative        | 1:20-11:45 |
| Federal Bureau of        |                             | a.m.       |
| Investigation            |                             |            |
+--------------------------+-----------------------------+------------+
|                          | LUNCH Provided in Hotel     | 12:00 p.m. |
|                          | lobby                       |            |
+--------------------------+-----------------------------+------------+
| **CLINICAL DIAGNOSIS     |                             |            |
| SESSION**                |                             |            |
+--------------------------+-----------------------------+------------+
| John Barr                | Analysis of Chemical and    | 1:00-1:40  |
|                          | Biological Weapons in Human | p.m.       |
| Centers for Disease      | Clinical Samples by Isotope |            |
| Control and Prevention   | Dilution Mass Spectrometry  |            |
+--------------------------+-----------------------------+------------+
| Bogumila Szponar         | 3-Hydroxy Fatty Acids as    | 1:45-2:05  |
|                          | Diagnostic Markers in       | p.m.       |
| University of Lund       | Chronic Periodontis         |            |
+--------------------------+-----------------------------+------------+
| K Udeni Alwis            | Validation of 3-Hydroxy     | 2:10-2:30  |
|                          | Fatty Acids for Large-Scale | p.m.       |
| Harvard School of Public | Epidemiological Studies of  |            |
| Health                   | Endotoxin Exposure          |            |
+--------------------------+-----------------------------+------------+
| **BREAK**                |                             | 2:35-2:50  |
|                          |                             | p.m.       |
+--------------------------+-----------------------------+------------+
| David C White            | Assessment of Regulatory    | 2:50-3:10  |
|                          | Lipids in Breath Condensate | p.m.       |
| University of Tennessee  | as Potential Presymptomatic |            |
|                          | Harbingers of Pulmonary     |            |
|                          | Pathology                   |            |
+--------------------------+-----------------------------+------------+
| Kathryn A Jackson        | Benchmarking and Validation | 3:15-3:35  |
|                          | of a Generic Intact Cell    | p.m.       |
| Shimadzu Biotech         | MALDI Strategy for the      |            |
|                          | Epidemiology of MRSA        |            |
+--------------------------+-----------------------------+------------+
| Lennart Larsson          | Use of Mass Spectrometry    | 3:40-4:00  |
|                          | for Studying Health         | p.m.       |
| University of Lund       | Consequences of Human       |            |
|                          | Exposure to Microorganisms  |            |
|                          | in the Environment          |            |
+--------------------------+-----------------------------+------------+
| **River Cruise/hors      |                             | 6:00 p.m.  |
| d/oeuvres** Tickets      |                             |            |
| Required Leave from      |                             |            |
| Marriott Deck            |                             |            |
+--------------------------+-----------------------------+------------+

**[Presenters and Titles]{.smallcaps}**

**[5^th^ International Symposium on the Interface]{.smallcaps}**

**[Between Analytical Chemistry and Microbiology]{.smallcaps}**

Wednesday, April 21, 2004

Courtyard Marriott Meeting Room, Richland, Washington

+--------------------------+-----------------------------+------------+
| **Presenter**            | **Title of Abstract**       | **Time**   |
+--------------------------+-----------------------------+------------+
| **ANALYTICAL METHODS     |                             |            |
| DEVELOPMENT, Part II**   |                             |            |
+--------------------------+-----------------------------+------------+
| Cynthia Bruckner-Lea     | Automated Methods for       | 8:30-9:05  |
|                          | Multiplexed Pathogen        | a.m.       |
| Timothy Straub           | Detection                   |            |
|                          |                             |            |
| Pacific Northwest        |                             |            |
| National Laboratory      |                             |            |
+--------------------------+-----------------------------+------------+
| Deborah A Kuzmanovic     | Evaluation of the           | 9:10-9:30  |
|                          | Integrated Virus Detection  | a.m.       |
| GEO-Centers, Inc.        | System (IVDS): A Novel      |            |
|                          | Instrument for Virus and    |            |
|                          | Microsphere Sizing and      |            |
|                          | Concentration Determination |            |
+--------------------------+-----------------------------+------------+
| Yolanda Fintschenko      | An Insulator-Based          | 9:35-9:55  |
|                          | (Electrodeless)             | a.m.       |
| Sandia National          | Dielectrophoretic           |            |
| Laboratory               | Concentrator for Microbes   |            |
|                          | in Water                    |            |
+--------------------------+-----------------------------+------------+
| **BREAK**                |                             | 1          |
|                          |                             | 0:00-10:15 |
|                          |                             | a.m.       |
+--------------------------+-----------------------------+------------+
| Charles L Wilkins        | High-Performance Mass       | 1          |
|                          | Spectrometry Applied to     | 0:20-10:40 |
| University of Arkansas   | Rapid Analysis of Bacteria  | a.m.       |
+--------------------------+-----------------------------+------------+
| Ljiljana Paša-Tolić      | Profiling Protein           | 1          |
|                          | Abundances Using LC-FTICR   | 0:45-11:05 |
| Pacific Northwest        | MS                          | a.m.       |
| National Laboratory      |                             |            |
+--------------------------+-----------------------------+------------+
| Hercules Moura           | MALDI-TOF MS Using          | 1          |
|                          | Fingerprinting Algorithms   | 1:10-11:30 |
| Centers for Disease      | and Biomarker               | a.m.       |
| Control and Prevention   | Identification              |            |
+--------------------------+-----------------------------+------------+
|                          |                             |            |
+--------------------------+-----------------------------+------------+
| Jacek P Dworzanski       | A Proteomic Approach for    | 1          |
|                          | Identification of Bacteria  | 1:35-11:55 |
| Geo-Centers, Inc.        | with Fully Sequenced        |            |
|                          | Genomes                     |            |
+--------------------------+-----------------------------+------------+
| **LUNCH** Provided in    |                             | 12:00 p.m. |
| Hotel lobby              |                             |            |
+--------------------------+-----------------------------+------------+
| **HOMELAND               |                             |            |
| SECURITY/BIODEFENSE      |                             |            |
| SESSION, Part II**       |                             |            |
+--------------------------+-----------------------------+------------+
| Matthias Frank           | Real-Time Detection and     | 1:00-1:20  |
|                          | Characterization of         | p.m.       |
| Lawrence Livermore       | Individual Bioaerosol       |            |
| National Laboratory      | Particles with Bioaerosol   |            |
|                          | Mass Spectrometry           |            |
+--------------------------+-----------------------------+------------+
|                          |                             |            |
+--------------------------+-----------------------------+------------+
| Ben van Baar             | Detection of *Clostridium   | 1:25-1:45  |
|                          | botulinum* Toxin in         | p.m.       |
| TNO Prins Maurits        | Biological Matrices Using   |            |
| Laboratory               | ELISA, Real-Time PCR and    |            |
|                          | Liquid                      |            |
|                          | Chromatography---Mass       |            |
|                          | Spectrometry                |            |
+--------------------------+-----------------------------+------------+
| Anne E Boyer             | Fast Detection and          | 1:50-2:10  |
|                          | Differentiation of          | p.m.       |
| Centers for Disease      | Botulinum Neurotoxins A, B, |            |
| Control and Prevention   | C, D, E and F, Using        |            |
|                          | MALDI-TOF Mass Spectrometry |            |
+--------------------------+-----------------------------+------------+
| **BREAK**                |                             | 2:15-2:30  |
|                          |                             | p.m.       |
+--------------------------+-----------------------------+------------+
| Leslie A Harden          | MALDI-TOF Mass Spectrometry | 2:35-2:55  |
|                          | Identification of           | p.m.       |
| U.S. Department of       | *Campylobacter* Species by  |            |
| Agriculture              | Fingerprint Analyses of     |            |
|                          | Whole-Cell Lysates          |            |
+--------------------------+-----------------------------+------------+
| Diane Dare               | MALDI-TOF Mass Spectrometry | 3:00-3:20  |
|                          | as a Tool for the Rapid and | p.m.       |
| Manchester Metropolitan  | Specific Identification of  |            |
| University               | *Bacillus* Pathogens        |            |
+--------------------------+-----------------------------+------------+
| Karen Wahl               | Microorganism               | 3:25-3:45  |
|                          | Identification Across       | p.m.       |
| Pacific Northwest        | Different Culture           |            |
| National Laboratory      | Conditions by MALDI Mass    |            |
|                          | Spectrometry                |            |
+--------------------------+-----------------------------+------------+
| **FINALE DINNER AT       |                             | 6:30 p.m.  |
| HOTEL** Complimentary    |                             |            |
+--------------------------+-----------------------------+------------+