**30^th^ Annual**

**Eastern Fish Health Workshop**

**13 -- 17 June, 2005**

**Clarion Inn and Conference Center**

**Shepherdstown, West Virginia**

**National Fish Health Research Laboratory**

**USGS - Leetown Science Center**

**11649 Leetown Road**

**Kearneysville, West Virginia 25430**

![](media/image1.jpeg){width="1.6041666666666667in"
height="0.6472222222222223in"}

**[Monday , June 13]{.underline}**

5:00 -- 7:30 pm **Registration And Reception**

Hors d'oeuvres and Cash Bar

Clarion Ballroom, Salon C

8:00 pm **Review Of Judging Criteria For Student Presentations**

Clarion Board Room

9:00 pm **Why Antietam?**

> (Background information of what led up to the battle
>
> and prelude to the battlefield tour.) Clarion Board Room

**[Tuesday, June 14]{.underline}**

7:00 **Breakfast** *Fresh fruit cocktail, scrambled eggs, bacon,
pancakes, home fries,*

> *assorted bakery items, coffee, tea, and milk*

**[General Session 1]{.underline}** - **Clifford Starliper, moderator**

> 8:00 Toby Lowry *Mycobacterium* sp. Infection In Cultured Cobia
> (*Rachycentron* 1
>
> *candum)*

8:15 Mark Sokolowski Mycobacterium Infections In New York Striped Bass
And Bluefish 2

8:30 Chris Ottinger Mycobacterial Infections In Striped Bass (*Morone
saxatilis*) From 3

> Delaware Bay

8:45 John Lumsden Coldwater disease caused by *Flavobacterium
psychrophilum* in 4

> Ontario; characterization of strains and antimicrobial resistance
>
> 9:00 Virginia Harper Detection Of Flavobacteria On Gills And Skin Of
> Salmonids by 5
>
> PCR And DGGE

9:15 Roselynn Stevenson Application Of DGGE Techniques As A Potential
Approach To 6

> Non-Lethal Testing For Fish Pathogens

9:30 Indervesh Signature-Tagged Mutants For Studies Of Pathogenic
Mechanisms 7

> Of *Yersinia ruckeri* In Rainbow Trout
>
> 9:45 Greg Wiens Estimation Of The Genome Size Of *Renibacterium
> salmoninarum* 8
>
> ATCC 33209 By Pulsed-Field Gel Electrophoresis

10:00 **Morning Break** *Coffee, tea*

**[Emerging Disease Concerns in Canadian Fisheries]{.underline} - David
Groman, moderator**

10:30 Dave Groman An Introduction To Canadian Aquaculture 9

10:45 Jan Lovy The Current Status Of *Loma* *salmonae*, Disease And
Control In 10

Farm-raised Chinook Salmon, *Oncorhynchus tshawytscha*

11:00 Daryl Whelan Emerging Infectious Diseases of Atlantic Cod (*Gadus
morhua*), 11

> Haddock (*Melanogrammus aeglefinus*) And Halibut (*Hippoglossus
> hippoglossus*)

11:15 Michael Beattie Multiple Drug Resistance In *Aeromonas
salmonicida* Infected 12

> Salmon Fry And An Update On Infectious Salmon Anemia
>
> In The Bay Of Fundy Region, Atlantic Canada

11:30 Molly Kibenge Identification of a new HPR group of infectious
salmon anemia 13

> virus in New Brunswick: What does it mean?

11:45 Rod Penney Current Status Of Canada's National Aquatic Animal
Health 14

> Program (NAAHP)

12:00 **Lunch** *House salad, vegetable lasagna, vegetables, rolls,
coffe, ice tea*

1:00 Frank Panek Welcome To The 30^th^ EFHW

Bill Palmisano

1:10 Jill Rolland The National Aquatic Animal Health Plan: Briefing And
Update 15

**[Health Of Native And Introduced Oysters In Chesapeake
Bay]{.underline} - Gene Burreson, moderator**

1:30 Eugene Burreson The Proposed Use Of The Non-Native Oyster
*Crassostrea* 16

> *ariakensis* In Chesapeake Bay

1:45 Kimberly S. Reece Detection And Identification Of Oyster Herpes
Viruses (OsHV) 17

> And *Perkinsus* Species From Oysters In Asia: Relevance To
>
> Chesapeake Bay

2:00 Ryan Carnegie Epizootiology Of *Bonamia* sp. In Non-Native Oysters,
*Crassostrea* 18

> *ariakensis*, In North Carolina, And Implications For Aquaculture

2:15 Corinne Audemard Salinity Effects On The Persistence Of *Bonamia*
sp. In *Crassostrea* 19

> *ariakensis*

2:30 Ryan Carnegie Polydora-Associated Shell Disease: Recent
Distribution In Native 20

> And Non-Native Oysters, And Implications For A *Crassostrea*
>
> *ariakensis* Introduction

2:45 Eugene Burreson Recent Trends In Distribution And Abundance Of
*Haplosporidium* 21

> *nelsoni* (MSX) And *Perkinsus marinus* (Dermo) In Native Oysters
>
> In Chesapeake Bay

3:00 **Afternoon Break** *Coffee, tea, sodas*

**[Coral Reef Health]{.underline} - DeEtta Mills, moderator**

3:30 DeEtta Mills Coral Diseases: An Overview And Update On Black Band
Disease 22

> In The Bahamas And The Florida Keys

3:45 Geoffrey Cook Comparison Of Bacterial Communities Between
Geographically 23

> Separated Corals Infected With White Plague Type II

4:00 Kathryn Sutherland Prevalence And Potential Origin Of The Acroporid
Serratiosis 24

> Pathogen In The Florida Keys

4:15 Garriet Smith The Role Of *Vibrio* spp. In Coral Disease: From
Normal Microbiota 25

> To Pathogens

4:30 Elizabeth Remily Use Of Fluorescence *In Situ* Hybridization For
Identification Of 26

> *Aurantimonas coralicida* In Environmental Samples

4:45 John Lisle Coral Mucous And Indigenous Bacteria: A Possible
First-Line 27

> Defense Against Coral Disease Pathogens

5:00 Andrew Routh Customs-Confiscated Corals: Gifthorses Requiring Oral
28

> Examination

5:15 Kathy Price Preparation Of Coral Tissue For Histological
Examination: A 29

Primer On Fixation And Decalcification

5:30 **Adjourn**

6:30 **Leetown Barbecue** *Fried chicken and ribs with the" fixins"*

**[Wednesday, June 15]{.underline}**

7:00 **Breakfast** *Fresh fruit cocktail, wester style eggs, sausage,
French toast, home*

> *fries, assorted bakery items, coffee, tea, and milk*

**[Control And Treatment Of Parasitic Infestations]{.underline} - Drew
Mitchell, moderator**

8:00 Drew Mitchell The Control Of Fish Parasites And The Eradication Of
Asian 30

> Tapeworm Infections In Grass Carp Using Praziquantel Bath
>
> Treatments

8:15 Michael Pietrak Sea Lice Control In The Maine Aquaculture Industry
31

8:30 Ed Noga The Role Of Antimicrobial Polypeptides In Resistance To
Parasitic 32

> Diseases Of Fish

8:45 Linda Pote Further Elucidation Of The Life Cycle And Pathology Of
The 33

> Digenetic Trematode, *Bolbophorus damnificus*

9:00 Al Camus Evaluation Of Copper Sulfate To Control Snail Numbers In
34

> Catfish Ponds Affected By *Bolbophorus* Trematodes

9:15 Michael Boogaard Integrated Control Program For The Sea Lamprey,
*Petromyzon* 35

> *marinus*, Macroparasite Of The Great Lakes

9:30 Susan Marcquenski Control Measures For *Heterosporis* Species 36

9:45 Walter Burak Some Effects Of *Philometra saltatrix* On Bluefish
Ovaries 37

10:00 **Morning Break** *Coffee, tea*

**[Diagnostic Case Reports: Part 1]{.underline} - Paul Bowser and
Mohamed Faisal, moderators**

10:30 Cynthia Stine Gross Observations From A Chesapeake Bay Fish Survey
38

10:40 Stephanie Grimmett Spinal Deformity In Triploid Grass Carp
*Ctenopharyngodon idella* 39

10:50 Lester Khoo Deaths Associated With Vascular Pathology In Hybrid
Striped Bass 40

11:00 Mohamed Faisal Widespread Deformities In An Inland Yellow Perch
Population 41

> In Michigan

11:10 Sal Frasca An Extreme Example Of Common Seahorse Diseases 42

11:20 Paul Bowser Pathology Associated With Large Cavernous Lesions In
Chinook 43

> Salmon From Lake Ontario

11:30 Gregory A. Wooster A Case Of Mistaken Sexual Identity And
Function: Pitfalls Of 44

> Methyl-Testosterone Sex-Reversal In Aquaculture

11:40 Thomas Loch Mass Mortalities In The Brook Stickleback (*Culaea
inconstans*) 45

> Exhibiting Abnormal Abdominal Tumors

11:50 Ana Baya Life Is Never Dull At College Park 46

12:00 **Lunch** *House salad, chicken marsala, potatoes, vegetables,
rolls, coffee, tea*

**[General Session 2]{.underline} - Phil McAllister, moderator**

1:00 Sndrew Sutherland Effects Of Elevated Suspended Sediment On Stress,
Growth And 47

> Gill Condition Of The Spotfin Chub And The Whitetail Shiner

1:15 Emmenegger, Evi Infectious Hematopoietic Necrosis Virus In Atlantic
Salmon 48 Seapens In British Columbia Lanie Bilodeau

1:30 Phil McAllister Virulence, Monoclonal Epitope, And Genome Analyses:
A 49

> Conjoined Assessment Of Infectious Pancreatic Necrosis Virus
>
> Isolates Endemic And Exotic To The Great Lakes Basin

1:45 Vanessa Kirsipuu Largemouth Bass Virus In New York State: An Update
50

2:00 Jeff Terhune First Report Of Koi Herpesvirus In Wild Common Carp In
The 51

> Western Hemisphere

2:15 Andrew Goodwin Diagnosis Of Goldfish Hematopoietic Necrosis
(*Cyprinid* 52

> *Herpesvirus 2*) By Quantitative PCR

2:30 Igor Shchelkunov Spring Viremia Of Carp Virus In Rainbow Trout:
Possible But 53

> Uncommon

2:45 Raghu Shivappa *In Vitro* Sensitivity And Specificity Analysis Of
Spring Viremia Of 54

> Carp Virus Diagnostic Tests

3:00 **Afternoon Break** *Coffee, tea, sodas*

**[Diagnostic Case Reports: Part 2]{.underline} - Paul Bowser and
Mohamed Faisal, moderators**

3:30 Jo Sadler Cutaneous Infection Of Channel Catfish By Microscopic
Nematodes 55

3:40 Thomas Loch Widespread Skin Ulcerations In The Yellow Perch
Population Of 56

> Lake St. Claire

3:50 Paul Bowser A Moribund Lake Sturgeon From The Niagara River, New
York 57

4:00 Stephanie Grimmett A Fish Kill Of Common Carp In New York State And
An 58

> Unsusual Koi Herpesvirus

4:10 Sal Frasca Encounters With Fungi In Sygnathid Fish 59

4:20 Alaa Eissa Furunculosis In The Sea Lamprey (*Petromyzon marinus*)
In 60

> Lake Ontario

4:30 Al Dove Lesions Caused By *Pomphorhynchus* spp. (Acanthocephala) In
61

> Bluefish And Striped Bass

4:40 Scott Fitzgerald An Outbreak Of Phaeohyphomycosis In
Hatchery-Reared 62

> Chinook Salmon Fingerlings

4:50 Jeffrey Wolf Livid Lesions In Lumpfish (*Cyclopterus lumpus*)
Associated With 63

> A Fungus *Exophiala pisciphila*

5:00 Julie Bebak-Williams Coldwater Disease Outbreak At A West Virginia
Rainbow Trout 64

> (*Oncorhynchus mykiss*)Farm: An Unusual Presentation

5:10 Christine Densmore Probable Branchiomycosis Among A Captive
Population Of 65

> Horseshoe Crabs , *Limulus polyphemus*, From Delaware Bay

5:20 **Adjourn**

> **Antietam National Battlefield Tour**

5:30 Car pool departs from the front door of the Clarion Inn at 5:30
sharp

9:00 Approximate end of tour, car pool returns to hotel

**[Thursday, June 16]{.underline}**

7:00 **Breakfast** *Fresh fruit cocktail, scrambled eggs, ham, pancakes,
home fries,*

> *assorted bakery items, coffee, tea, and milk*

**[General Session 3]{.underline}** - **Chris Ottinger, moderator**

8:00 Stephen Kaattari The Role Of The Anterior Kidney In Generating
Long-Term 66

> Immunity

8:15 Ilsa Kaattari Development Of *In Vitro* Methods To Determine
Antibody Gene 67 Specificity In Salmonids

8:30 Jianmin Ye Unique Mechanisms Employed By Fish To Generate Antibody
68

Functionality

8:45 Erin Bromage Unique Difficulties In The Development Of Serological
Reagents 69

> For Striped Bass Antibody Responses

9:00 Don Evans Mechanisms Of Antibacterial Resistance By Nonspecific
Cytotoxic 70

> Cells

9:15 Liliana Jaso-Friedmann Effector Molecules Of Cell Mediated
Cytotoxicity In Catfish And 71

> Tilapia NCC

9:30 Don Ourth Mannose-Binding Lectin Of Channel And Blue Catfish 72

9:45 Spencer Russell Plasma Proteomic Analysis Of The Acute Phase
Response Of 73

> Rainbow Trout To Intraperitoneal Inflammation And LPS Injection

10:00 **Morning Break** *Coffee, tea*

**[Managing Health And Disease In Commercial Aquaria]{.underline} - Jill
Arnold**

10:30 Catherine Hadfield Cross-matching Of Elasmobranch And A
Transfusion Trial Within 74

Atlantic Stingrays (*Dasyatis sabina*)

10:45 Scott Weber Reproductive Problems, Diagnoses And Treatments In
Teleosts 75

11:00 Mohammad Alavi Ecology Of The Fish Pathogen *Mycobacterium
marinum* 76

11:15 Andrew Kane Mycobacteriosis In Wild-Caught, Laboratory-Maintained
Menhaden: 77

A Case Study

11:30 Andy Aiken Removal Of Chloramines From The Source Water At The
National 78

> Aquarium - Baltimore

11:45 Brent Whitaker Disaster Planning For Aquatic Animal Facilities 79

12:00 **Lunch** *Trio plate of* *shrimp salad, tuna salad, and chicken
salad on bed of*

> *greens garnished with sweet bread and cottage cheese, rolls, coffee,
> tea*

**[General Session 4]{.underline} -- Stephen Smith , moderator**

1:00 Patricia Barbash Cooperative Investigations Of Suspect Disease
Cases In Atlantic 80

> Sturgeon (*Acipenser oxyrinchus oxyrinchus*)

1:15 Rocco Cipriano Assessing Susceptibility Of Atlantic Sturgeon
(*Acipenser* 81

> *oxyrinchus*) To *Aeromonas salmonicida*, Cause Of Furunculosis

1:30 Karrie Young Pathogen Binding Proteins In Plasma Of Salmonid Fish
82

1:45 Ahmed Darwish Laboratory Efficacy Of Amoxicillin For The Control Of
83

> *Streptococcus iniae* Infection In Blue Tilapia

2:00 Gregory Broussard Development Of A Tuberculosis Model Using The
Small Fish 84

Medaka (*Orysias latipes*) And *Mycobacterium marinum*

2:15 Andrew Draghi Localization By *In Situ* Hybridization Of A
Chlamydia-Like 16S 85

> Ribosomal RNA Gene Sequence Within Branchial Epithelial Cells
>
> Of Cultured Arctic Char (*Salvelinus alpinus*) With Epitheliocystis

2:30 Xiguang Zhao Quantitation Of Immobilization Antigen-Specific
Antibody 86

> Secreting Cells In The Skin And Head Kidney Of *Ichthyophthirius
> multifiliis* Immune Channel Catfish Using ELISPOT Assay

2:45 Luke Iwanowicz Intersex In Smallmouth Bass From The South Branch Of
The 87

> Potomac River: Implications Of Endocrine Disruption

3:00 **Afternoon Break** *Coffee, tea, sodas*

**[General Session 5]{.underline} - Alistair Dove, moderator**

3:30 Clifford Starliper Bacteriological Studies On Freshwater Mussels:
The Flora Present 88

> In Free-Ranging Animals And Pathogen Depuration By Two Species,
>
> *Amblema plicata* And *Fusconaia ebena*

3:45 Amanda Tribble Roles Of Astaxanthin In The Pathobiology Of
Lobsters, *Homarus* 89

> *americanus*

4:00 Ann Zulkosky Toxicopathology Of Resmethrin In Larval And Juvenile
American 90

> Lobsters (*Homarus americanus*)

4:15 Stephen Smith Laboratory Culture And Health Care Of The Horseshoe
Crab 91

> (*Limulus polyphemus*)

4:30 Earl J. Lewis Histological Techniques For Marine Bivalve Mollusks
And 92

> Crustaceans

4:45 Cathy Czerwinski Plasma Cortisol In White Perch *Morone americana*:
Associations 93

> With Sampling Method, Dissolved Oxygen, And Water
>
> Temperature

5:00 **Adjourn**

7:00 **Banquet and Bar** Two complimentary cocktails, *California green
salad, Caesar salad,*

> *rotisserie style chicken, chef's hoice of vegetarian dish, carved
> roast top round of beef, vegetables, potatoes, rolls, coffee, tea
> pies, cake, mousse and fresh fruit*

8:30 **Presentation of Best Student Paper Award**

**Dance and Cash Bar**

11:30 **Adjourn**

**[Friday, June 17]{.underline}**

**[For Registrants Of The Spring Viremia Of Carp Continuing Education
Course Only]{.underline}**

7:00 Breakfast *Fresh fruit cocktail, western style eggs, bacon, French
toast, home*

> *fries, assorted bakery items, coffee, tea, and milk*

8:00 **[Spring Viremia of Carp Continuing Education Course -- Andy
Goodwin]{.underline}**

**Presentors:**

> *[Andy Goodwin]{.underline}*, University of Arkansas at Pine Bluff, An
> overview of SVCV in the
>
> United States and an introduction to some of the important questions
> without answers
>
> *[Barry Hill]{.underline},* CEFAS, Weymouth, UK, Impacts, control and
> regulation of SVCV in wild
>
> and cultured fish in western Europe with a history of SVCV strains
> from China
>
> *[Igor Shchelkunov]{.underline}*, Russia, Impacts, regulation and
> control of SVCV on wild and cultured
>
> fish in Eastern Europe
>
> *[David Stone]{.underline}*, CEFAS, Weymouth, UK, Molecular biology
> and taxonomy of SVCV:
>
> relationships between SVCV (European and Asian), PFRV, and other
> similar
>
> rhabdoviruses
>
> *[Andy Goodwin]{.underline}*, University of Arkansas at Pine Bluff.
> Diagnosis of SVCV. PCR and
>
> serological tests. What they tell us and what they don\'t
>
> *[Sue Marcquenski]{.underline}*, Wisconsin, DNR SVCV and the wild fish
> of Wisconsin
>
> *[Robert Bakal]{.underline},* USFWS, Warm Springs, GA, SVCV and the
> wild fish of North Carolina
>
> and Virginia
>
> *[Jill Rolland]{.underline}*, APHIS, The current and future regulation
> of SVCV in the United States

10:00 **Morning Break** *coffee, tea*

12:00 **Lunch** *House salad and dressing, char-grilled beef kabob
served with rice*

> *pilaf, rolls, coffee, tea*

2:30 **Afternoon Break** *Coffee, tea, sodas*

4:00 **Adjourn**

**[Editorial Note]{.underline}**

Once again, the staff of the National Fish Health Research Laboratory
welcomes you to the 30^th^ Annual Eastern Fish Health Workshop here at
the Clarion Inn and Conference Center, Shepherdstown, West Virginia.
Once again, we are happy to extend our hospitality to you in this
beautiful facility. Once again, we are happy to convene this workshop in
service to the scientific community and in friendship to each of you,
who have joined us this year.

It has now been thirty years since a small group of federal researchers
gathered on the Eastern Shore of Maryland to share their research,
discuss problems, plan new studies, and enjoy the fellowship of each
other. That first meeting was originally inspired by Dr. Stanislaus
Snieszko of the Eastern Fish Disease Laboratory (predecessor to the
National Fish Health Research Laboratory) of the Department of the
Interior and Drs. Carl Sinderman and Aaron Rosenfeld of the National
Marine Fisheries Service Laboratory of the Department of Commerce at
Oxford, Maryland. Both laboratories were somewhat close geographically
and both researched problems associated with fish disease, but their
emphasis differed between the freshwater and marine environment. The
early workshops were a focal point that fostered interactions between
the two facilities and provided staff members with opportunities to plan
and conduct collaborative studies. Within a few short years, fish
disease research became well established in the graduate programs of
several major universities in the eastern United States and
participation in the workshop evolved beyond its original intent.

Today, thirty years later, the workshop continues to thrive. It has
witnessed the evolution of a profession and it has witnessed the comings
and goings of so many individuals who have been vital to its success.
The workshop prospers because it has evolved to provide a valuable
scientific forum presented in an atmosphere of friendship. It has
evolved from fish and shellfish to a broad participation in all aspects
of aquatic animal health. Yet, the workshop remains true to its roots
and encourages contributions from researchers, resource managers,
biologists, aquarists, veterinarians, university personnel, and students
working within both marine and freshwater environments. Though the
Workshop has occasionally been held in conjunction with other
organizations such as the Fish Health Section of the American Fisheries
Society and the International Association of Aquatic Animal Medicine, it
is not affiliated with any parent organization and the meeting continues
to be an entity unto itself. Today, just like thirty years ago, the
National Fish Health Research Laboratory is extremely proud to celebrate
another chapter in the history of this workshop as we gather to
celebrate its 30^th^ Anniversary. We hope that you enjoy the
proceedings, the friendship, and the beauty of the local surroundings.
Should the past be prologue to the future, we anticipate many more such
gatherings.

##### 

##### Rocco C. Cipriano