[Answers]{.underline}

[Testing for Aflatoxin]{.underline}

**A**. **[General Questions]{.underline}**

1\. (a) True

2\. (b) 10 pounds

3\. (a) True

4\. (b) Measuring the level of aflatoxin against a set threshold such as
20 ppb.

5\. (a) True

6\. (a) 2 pounds

7\. (b) 500 grams

8\. (b) False

9\. (b) 3 pounds

10\. (d) All of the above

11\. (a) True

12\. (a) True

13\. (d) Qualitative and Quantitative

14\. (b) 10 pounds

15\. (a) True

16\. (b) Quantitative methodology.

**B. [Vicam Aflatest Specific Questions]{.underline}**

1\. (b) Stored in a cabinet or drawer in the original case when not in
use.

2\. (a) Wipe each of the vials with a clean cloth or paper wipe.

3\. (a) True

4\. (a) True

5\. (b) False

6\. (a) 45

7\. (d) Every hour

8\. (b) +1.0

9\. (b) False

10\. (a) True

11\. (c) Background fluorescence

**C. [Neogen AgriScreen Specific Questions]{.underline}**

1\. (a) True

2\. (a) True

3\. (a) Blue color

4\. (a) True

5\. (c) 50 grams of ground sample be placed in a whirlpak with 250 ml of
70% methanol/water solution and mixed by shaking by hand for 3 minutes.

6\. (a) One day

7\. (a) True

8\. (a) True

9\. (d) All of the above

10\. (a) True

11\. (a) True

12\. (a) True

**D. [Neogen Veratox AST Specific Questions]{.underline}**

1\. (d) All of the above.

2\. (b) False

3\. (b) Blending on high speed for exactly 2 minutes.

4\. (c) Three -- one control and two sample portions.

5\. (a) True

6\. (c) 50 grams of ground sample be mixed with 250 ml of 70%
methanol/water solution and blended on high speed for exactly 2 minutes.

7\. (b) With a wash bottle containing deionized/distilled water, fill
each antibody well and dump the contents into a waste receptacle. Repeat
this step 5 times.

8\. (c) Refrigerated at temperatures between 36°- 46° F.

9\. (c) Gently sliding the microwell holder back and forth on a
horizontal surface for 15

seconds.

10\. (b) Two

11 (b) Contact the Neogen Corporation representative for instruction.

**E. [Romer Fluoroquant FQ Specific Questions]{.underline}**

1\. (a) True

2\. (a) 50 mL

3\. (b) Three times

4\. (c) 8

5\. (c) At least 30 - 40 seconds

6\. (c) 1.0 mL

7\. (a) 1.0 mL

8\. (d) 5 seconds

9\. (b) 1.0 mL

10\. (a) One

11\. (a) True

12 (b) False

13\. (d) Refrigerated at 23° to 32° F

14\. (a) 50 grams of ground sample is mixed with 100 mL of 80%
methanol/water

> solution and blended on high speed for one minute.

15\. (c) 50 grams of ground sample is placed in a whirlpak with 100 mL
of 90%

> acetonitrile/water solution and blended on high speed for one minute.

**F. [SDI Myco ]{.underline}** **[Specific Questions]{.underline}**

1\. (c) 50 grams of ground sample be mixed with 250 ml of 70%
methanol/water solution and blended on high speed for exactly 2 minutes.

2\. (d) nineteen

3\. (a) One

4\. (c) At temperatures between 36° - 46° F.

5\. (a) True

6\. (a) True

7\. (b) four

8\. (d) Hyperion MicroReader 3 well reader

9\. (b) 475

10\. (a) exactly five minutes

11\. (a) True

**G.** **[R -Biopharm Ridascreen FAST Aflatoxin SC- Specific
Questions]{.underline}**

1\. (c) [distilled or deionized]{.underline}

2\. (a) [50 grams]{.underline}

3\. (b) [two]{.underline}

4\. (b) [1.5]{.underline}

5\. (a) [one]{.underline}

6\. (d) [23]{.underline}

7\. (b) [ten (± 1.0 minutes)]{.underline}

8\. (d) [Both (a) and (c)]{.underline}

9\. (c) [ten]{.underline}

10\. (b) [False]{.underline}

11\. (a) [True]{.underline}

12\. (a) [True]{.underline}

**H.** **[Envirologix QuickTox- Specific Questions]{.underline}**

1\. (b) False

2\. (c) distilled or deionized

3\. (a) 50 grams

4\. (b) two

5\. (b) one minute

6\. (a) True

7\. (b) False

8\. (a) True

9\. (b) five minutes

10\. (a) True

11\. (b) False

12\. (a) True

**I.** **[R-Biopharm AFLACARD T20 -- Specific Questions]{.underline}**

1.  \(a\) True

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) one

6\. (a) True

7\. (c) 200 µl

8\. (a) True

9\. (b) False

10\. (a) True

11\. (a) True

12\. (b) False

**J [Neogen REVEAL FOR AFLATOXIN -- Specific Questions]{.underline}**

1\. (a) True

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) one

6\. (a) True

7\. (c) 200 µl

8\. (b) False

9\. (a) True

10\. (a) True

11\. (a) True

12\. (b) False

K.  **[Romer AGRASTRIP TEST KIT -- Specific Questions]{.underline}**

1\. (a) True

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) one

6\. (a) True

7\. (b) 50 µl

8\. (a) True

9\. (a) True

10\. (a) True

11\. (a) True

12\. (b) False

**L. [Neogen Veratox Specific Questions]{.underline}**

1\. (c) distilled or deionized

2\. (a) 50 grams

3\. (b) Blending on high speed for exactly 2 minutes.

4\. (a) True

5\. (d) Five -- Four controls and one sample portion.

6\. (d) twenty

7\. (c) Gently sliding the microwell holder back and forth on a
horizontal surface for 10 to 20 seconds.

8\. (b) With a wash bottle containing deionized/distilled water, fill
each antibody well and dump the contents into a waste receptacle. Repeat
this step 5 times.

9\. (b) False

10\. (a) True

11\. (c) Refrigerated at temperatures between 36°- 46° F.

12\. (b) False

**M. [Charm Science ROSA® P/N Specific Questions]{.underline}**

1\. (a) True

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) one

6\. (a) True

7\. (c) 100 µl

8\. (b) False

9\. (a) True

10\. (a) True

11\. (a) True

12\. (a) True

**N. [Charm Science ROSA® Aflatoxin (Quantitative) Specific
Questions]{.underline}**

1\. (b) False

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) 150 ml.

6\. (a) True

7\. (c) 100 µl

8\. (a) True

9\. (b) False

10\. (a) True

11\. (a) True .

12\. (b) False

**O.** **[Envirologix QuickTox (Released 2006) - Specific
Questions]{.underline}**

1\. (b) False

2\. (c) ethanol

3\. (a) 50 grams

4\. (b) two

5\. (b) one minute

6\. (a) True

7\. (a) True

8\. (a) True

9\. (b) five minutes

10\. (a) True

11\. (b) False

12\. (a) True