**High-throughput Microarray Processing**

![](media/image1.jpeg){width="2.2006944444444443in" height="0.8375in"}

The IGSB HGAC performs tiling and expression microarray hybridizations
using the Tecan HS 4800 PRO Hybridization Station and the Agilent manual
SureHyb protocol. Our facility has the current capacity to process 12
slides simultaneously on Tecan and 48 slides simultaneously following
Agilent's SureHyb protocol. To date we have processed Agilent arrays in
a high-throughput format following Agilent's Gene Expression or ChIP-seq
[protocols](http://www.chem.agilent.com/en-US/Products/Instruments/dnamicroarrays/pages/gp11617.aspx).
We have two scanners, a single slide Axon 4000B 5um scanner and a
C-version Agilent Autoloader scanner capable of scanning up to 48-slides
at a maximum resolution of 2um.

### Instruments

### [**Agilent C-Version DNA Microarray Scanner**](http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/dnamicroarrayscanner/pages/default.aspx)

### ![](media/image2.png){width="2.34375in" height="1.9791666666666667in"}

### [**Agilent Hybridization Oven (2)**](http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/dnamicroarrayhybridizationoven/pages/default.aspx)

### [![](media/image3.png){width="2.34375in" height="1.9791666666666667in"}](http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/dnamicroarrayhybridizationoven/pages/default.aspx)

### [**Agilent Microarray Hybridization Chamber -- SureHyb (48)**](http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/dnamicroarrayhybridizationchamber-surehyb/pages/default.aspx)

### [![](media/image4.jpeg){width="2.34375in" height="1.9791666666666667in"}](http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/dnamicroarrayhybridizationchamber-surehyb/pages/default.aspx)

###  [**Agilent 2100 Bioanalyzer**](http://www.chem.agilent.com/en-us/products/instruments/lab-on-a-chip/2100bioanalyzer/pages/default.aspx)

### ![](media/image5.jpeg){width="2.34375in" height="1.9791666666666667in"}

###  [**Tecan HS4800Pro**](http://www.tecan.com/platform/apps/product/index.asp?MenuID=1791&ID=1840&Menu=1&Item=21.10.1)

[![](media/image6.png){width="4.666666666666667in"
height="3.1284722222222223in"}](http://www.tecan.com/platform/apps/product/index.asp?MenuID=1791&ID=1840&Menu=1&Item=21.10.1)

### [**Molecular Devices Axon 4000B Single Slide Microarray Scanner**](http://www.moleculardevices.com/pages/instruments/gn_genepix4000.html)

### ![](media/image7.jpeg){width="1.875in" height="1.875in"}

**Contact Information**

Questions relating to the HGAC including pricing should be directed to
Marc Domanus, Laboratory Research Manager as described below.

Email: <sequencing@igsb.anl.gov>

IGSB High-throughput Genome Analysis Core (HGAC)

[Argonne National Laboratory](http://www.anl.gov/Visiting/anlemap.html)

9700 South Cass Avenue

Building 202 A353

Argonne, IL 60439-4803

Phone: (630) 252-3993

Fax: (630) 252-6333

LINKS

> Platforms
>
> [Roche GS FLX (454) Sequencing]{.underline}
>
> [Illumina Solexa Next Generation Sequencing]{.underline}
>
> [Microarray Processing]{.underline}
>
> [Sample Submission Guidelines]{.underline}