REPORTS OF THE COORDINATORS

**Overall coordinator's report**

**Udda Lundqvist**

SvalöfWeibull AB

SE-268 81 Svalöv, Sweden

e-mail: <udda@ngb.se>

Since the latest overall coordinator's report in Barley Genetics
Newsletter Volume 33, not many important changes of the coordinators
have been reported. I do hope that most of you are willing to continue
with this work and provide us with new important information and
literature search in the future. Unhappily some of the coordinators have
definitely retired from their positions or they do not find the time to
prepare reports because of other commitments, or they have stopped
working on barley research. The coordinator for Chromosome 3H, Roger
Ellis, has retired from his position at the Scottish Crop Research
Institute, United Kingdom, and we need to find a replacement. Diter von
Wettstein, the coordinator for Chloroplast genes has desired to pass
over this duty to Mats Hansson at the Department of Biochemistry, Lund
University, Lund, Sweden. He promised to make this coordination as he
intensively works on these problems. The coordinators for the
Inversions, Bengt-Olle Bengtsson and Torbjörn Säll, both from the
Institute of Genetics at the University of Lund, Sweden, asked to resign
as they are not working with barley genetic research any more. I want to
take the opportunity to thank all of them for their good corporation and
their reliability of sending informative reports during all the years.

At the end of June, many of us met at the 9th International Barley
Genetics Symposium in Brno, Czech Republic, and during a 'Barley Genetic
Linkage Workshop' where it got intensively discussed if the current
system and trait coordination should continue in this manner. I became
decided to do so but with a view towards whole genome coordination in
the future. The report of this workshop will be published in this or
next BGN Volume.

Problems of minor modifications of Rule 6 and 7 of Gene Nomenclature
were discussed and accepted at the Barley Genetic Linkage Workshop of
the 9^th^ International Barley Genetics Symposium in Brno, Czech
republic, on June 19, 2004. Rules for Nomenclature and Gene
Symbolization in Barley with the additional amendments will be published
in this volume. Revised lists of BGS descriptions by BGS number
(Table 1) and by locus symbols in alphabetic order (Table 2) are also
published in this volume.

## List of Barley Coordinators

**Chromoosome 1H (5):** Gunter Backes, Plant Research Department, Risø
National Laboratory, PRD-330, P.O.49, DK-4000 Roskilde, Denmark. FAX:
+45 46 77 4282; e-mail: \<[gunter.backes@risoe.dk\>]{.underline}

**Chromosome 2H (2):** Jerry. D. Franckowiak, Department of Plant
Sciences, North Dakota State University, P.O.Box 5051, Fargo, ND
58105-5051, USA. FAX: +1 701 231 8474; e-mail:
\<[j_franckowiak@ndsu.nodak.edu]{.underline}\>

**Chromosome 3H (3):** Roger P. Ellis, Cell and Molecular Genetics
Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2
5DA, United Kingdom. FAX: +44 1382 562426. E-mail:
\<R.Ellis@scri.sari.ac.uk\>

**Chromosome 4H (4):** Brian P. Forster, Cell and Molecular Genetics
Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2
5DA, United Kingdom. FAX: +44 1382 562426. e-mail:
\<[bforst@scri.sari.ac.uk]{.underline}\>

**Chromosome 5H (7):** George Fedak, Eastern Cereal and Oilseed Research
Centre, Agriculture and Agri-Food Canada, ECORC, Ottawa, ON, Canada K1A
0C6, FAX: +1 613 759 6559; e-mail: \<[fedakga@agr.gc.ca]{.underline}\>

**Chromosome 6H (6):** Duane Falk, Department of Crop Science,
University of Guelph, Guelph, ON, Canada, N1G 2W1. FAX: +1 519 763 8933;
e-mail: \<[dfalk@uoguelph.ca]{.underline}\>

**Chromosome 7H (1):** Lynn Dahleen, USDA-ARS, State University Station,
P.O. Box 5677, Fargo, ND 58105, USA. FAX: + 1 701 239 1369; e-mail:
\<[DAHLEENL@fargo.ars.usda.gov]{.underline}\>

**Integration of molecular and morphological marker maps:** Andy
Kleinhofs, Department of Crop and Soil Sciences, Washington State
University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail:
\<[andyk@wsu.edu]{.underline}\>

**Barley Genetics Stock Center:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<[anhang@uidaho.edu]{.underline}\>

**Trisomic and aneuploid stocks:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<[anhang@uidaho.edu]{.underline}\>

**Translocations and balanced tertiary trisomics:** Andreas Houben,
Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3,
DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail:
\<houben@ipk-gatersleben.de\>

**Desynaptic genes:** Andreas Houben, Institute of Plant Genetics and
Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany.
FAX: +49 39482 5137; e-mail: \<houben@ipk-gatersleben.de\>

**Autotetraploids:** Wolfgang Friedt, Institute of Crop Science and
Plant Breeding, Justus-Liebig-University, Heinrich-Buff-Ring 26-32,
DE-35392 Giessen, Germany. FAX: +49 641 9937429; e-mail:
\<[wolfgang.friedt@agrar.uni-giessen.de]{.underline}\>

**Eceriferum genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX:.+46 418 667109; e-mail:
\<[udda@ngb.se]{.underline}\>

**Chloroplast genes:** Diter von Wettstein, Department of Crop and Soil
Sciences, Genetics and Cell Biology, Washington State University,
Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail:
\<[diter@wsu.edu]{.underline}\>

**Genetic male sterile genes:** Mario C. Therrien, Agriculture and
Agri-Food Canada, P.O. Box 1000A, R.R. #3, Brandon, MB, Canada R7A 5Y3,
FAX: +1 204 728 3858; e-mail: \<MT[herrien@agr.gc.ca]{.underline}\>

**Inversions:** Bengt-Olle Bengtsson, Institute of Genetics, University
of Lund, Sölvegatan 29, SE-223 62 Lund, Sweden. FAX: +46 46 147874;

e-mail: \<[bengt_olle.bengtsson@cob.lu.se]{.underline}\> and

Torbjörn Säll, Institute of Genetics, University of Lund, Sölvegatan 29,
SE-223 62 Lund Sweden. FAX: +46 46 147874,
e-mail:\<[torbjorn.sall@cob.lu.se]{.underline}\>

**Ear morphology genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX: +46 418 667109; e-mail:
\<[udda@ngb.se\>]{.underline}

**Semi-dwarf genes:** Jerry D. Franckowiak, Department of Plant
Sciences, North Dakota State University, P.O. Box 5051, Fargo, ND
58105-5051, USA. FAX: +1 702 231 8474; e-mail:
\<[j_franckowiak@ndsu.nodak.edu]{.underline}\>

**Early maturity genes:** Udda Lundqvist, Svalöf Weibull AB, SE-268 81
Svalöv, Sweden. FAX: +46 418 667109; e-mail:
\<[udda@ngb.se]{.underline}\>

**Biochemical mutants - Including lysine, hordein and nitrate
reductase:** Andy Kleinhofs, Department of Crop and Soil Sciences,
Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509
335 8674; e-mail: \<[andyk@wsu.edu]{.underline}\>

**Barley-wheat genetic stocks:** A.K.M.R. Islam, Department of Plant
Science, Waite Agricultural Research Institute, The University of
Adelaide, Glen Osmond, S.A. 5064, Australia. FAX: +61 8 8303 7109;
e-mail: \<<rislam@waite.adelaide.edu.au>[\>]{.underline}

**Disease and pest resistance genes:** Brian Steffenson, Department of
Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper
Buford Circle, St. Paul, MN 55108-6030, USA. FAX: +1 612 625 9728;
e-mail: \<[bsteffen@umn.edu]{.underline}\>

**Coordinator's Report: Barley Chromosome 1H (5)**

**Gunter Backes**

**Risø National Laboratory, Plant Research Department**

**Resistance Biology Programme PRD 339**

**P.O. Box 49, DK-4000 Denmark**

Long *et al.*, 2003 performed a marker regression based on a segregating
population of 110 doubled haploid lines, derived from the cross
'Mundah/Keel'. Grain yield and early dry matter production were
associated with the chromosome 1H fragment containing the SSR markers
Bmac32 and Ebmac501. For the chromosome 1H fragment with the AFLP
markers P13/M49-251 and the SSR marker Awbma35, they found associations
with growth habit, early maturity, kernel yield, kernel weight and
kernel screening fractions.

Read *et al.*, 2003 localised a QTL for heading date on chromosome 1H in
166 DH lines from a cross between the varieties 'Sloop' and 'Halicon'.
The QTL was localised between the SNP locus SOUISC4 and the
microsatellite locus Bmac0382. The marker explained 28 % of the
phenotypic variance.

Pillen *et al.*, 2003 searched for associations between microsatellite
markers and agronomic traits in 136 BC~2~F~2~ lines from an advanced
backcross involving the wild barley (*Hordeum vulgare* ssp.
*spontaneum*) line '101-23' and the barley variety 'Apex'. They found
the following associations as expression of putative QTLs on chromosome
1H: an association between the SSR marker GMS21 and heading date,
kernels per ear and kernel yield, an association between heading date
and the SSR marker Bmac0213, an association between the traits heading
date, plant height and lodging with the microsatellites HvALAAT, HVM20
and HVM64 and finally between the same traits and Bmag0211. For all
QTLs, apart from the kernel yield related ones mentioned first, the
alleles from the wild line were more favourable in relation to common
breeding goals.

Mickelson *et al.*, 2003 studied nitrogen storage and remobilization in
barley leaves in a RI-population based on a cross between the two barley
varieties 'Karl' and 'Lewis'. On chromosome 1H, two regions of interest
were detected: one QTL for "total leaf nitrogen at mid-grain fill" near
the RFLP marker ABG53 and one QTL near the AFLP marker TB2122 for the
traits "total leaf nitrogen at anthesis", "total leaf nitrogen at
maturity" and yield.

Teulat *et al.*, 2003 found a locus with QTL x environment interaction
for relative water content near the locus for black pericarp colour
(*bpc*) on the long arm of chromosome 1H. The localisation was carried
out in 167 recombinant inbred lines (RILs) from the cross
'Tadmor/Er-Apm', and based on data of humid as well as drought
environments.

Also targeting towards genes of interest for barley cultivation under
drought conditions, Baum *et al.*, 2003 were localising QTL for
agronomic traits under mild and heavy drought stress. The analysis was
based on 194 RIL lines from a cross between the ICARDA-variety 'Arta'
and a *Spontaneum* line. Five QTLs were found on chromosome 1H: one QTL
near the SSR marker Bmag0105 for biological yield and plant height, on
just beside for growth habit and kernel weight, but only under favourite
conditions, one for biological yield and tiller number, one for growth
habit and finally one QTL for kernel weight, biological yield and tiller
number.

In 150 DH lines from 'Steptoe/Morex', Han *et al.*, 2003 localised a QTL
for acid detergent fibre (ADF). The locus was detected in the interval
between the markers AGA006 and *Hor2* and accounted for 23.6 % of the
variation for this trait.

Three QTLs for resistance against *Fusarium* head blight on chromosome
1H were localised by Dahleen *et al.*, 2003 in a DH population(75 lines)
resulting from the three-way cross 'Zhedar 2/ND9712//Foster'. Those
three QTLs were environment-specific.

In an attempt to analyse the relation of stoma density and "avoidance"
against *Puccinia hordei*, Vaz Patto *et al.*, 2003 localised QTLs for
the respective QTLs in a population of 100 F~2~ plants derived from a
cross between two *Hordeum chilense* accessions. On chromosome 1H, they
detected a QTL for "avoidance". There was no correlation between stoma
density and "avoidance".

Madsen *et al.*, 2003 described the development and localisation of
Resistance Gene Analogues (RGAs) for barley. On chromosome 1H, three
RGAs were localized: two (S-9217 and S-112, both between the RFLP locus
MWG55 and the SSR locus Bmag0211) in the mapping population '1B-87/Vada'
(Backes, *et al.*, 2003) and one (S-9240B, between the RFLP markers
PSB67 and WG518) in the mapping population 'Igri/Triumph' (Laurie *et
al.*, 1995).

In the above mentioned mapping population derived from a cross between
the *Spontaneum*-line '1B-87' and the barley variety 'Vada' (121 RI
lines), Backes *et al*,*.* 2003 localised a QTL for quantitative
resistance against powdery mildew (caused by *Blumeria graminis*) acting
additively in the field experiment at or nearby the *Mla* locus
conferring qualitative resistance against the same disease.

Collins *et al.* used four different mapping populations for the
localisation of QTLs for malt extract: a DH-population from the cross
'Sloop/Alexis' (Barr *et al.*, 2003), a RI-population from the cross
'Sloop-sibling/Alexis' (Barr *et al.*, 2003), a DH-population from the
cross 'Galleon/Haruna Nijo' (Karakousis *et al.*, 2003) and a
DH-population from the cross 'Chebec/Harrington' (Barr *et al.*, 2003).
On chromosome 1H, they detected two QTLs: one near the centromere in the
mapping populations Sloop/Alexis' (near the microsatellite locus
Ebmac0501) and 'Sloop-sibling/Alexis' and one on the long arm in
'Chebec/Harrington' (near the RFLP locus BCD508).

Clancy *et al.*, 2003 localised QTLs for beta-amylase activity
simultaneously in the three segregating populations derived from the
crosses 'Steptoe/Morex', 'TR306/Harrington' and 'Harrrington/Morex',
ranging from 144 to 150 doubled haploid (DH) lines. In two of the three
crosses, they found a major QTL for beta-amylase activity on the short
arm of chromosome 1H close by the *Hor1* locus. An additional minor QTL
for beta-amylase activity and diastatic power was detected on the same
chromosome, but only in 'Steptoe/Morex'.

In a DH population of 65 lines from the cross 'Tallon/Kaputar', Cakir
*et al.*, 2003 localised a QTL for diastatic power on chromosome 1H.

**References:**

Backes, G., L.H. Madsen, H. Jaiser, J. Stougaard, M. Herz, V. Mohler and
A. Jahoor. 2003. Localisation of genes for resistance against *Blumeria
graminis* f. sp. *hordei* and *Puccinia graminis* in a cross between a
barley cultivar and a wild barley (*Hordeum vulgare* ssp. *spontaneum*)
line. Theor. Appl. Genet. 106:353-362.

Barr, A.R., A. Karakousis, R.C.M. Lance, S.J. Logue, S. Manning, K.J.
Chalmers, J.M. Kretschmer, J.R. Boyd, H.M. Collins, S. Roumeliotis, S.J.
Coventry, D.B. Moody, B.J. Read, D. Poulsen, C.D. Li, G.J. Platz, P.A.
Inkerman, J.F. Panozzo, B.R. Cullis, D.B. Smith, P.Lim and P. Langridge.
2003. Mapping and QTL analysis of the barley population Chebec ×
Harrington. Aust. J. Agr. Res. 54:1125-1130.

Baum, M., S. Grando, G. Backes, A. Jahoor, A. Sabbagh and S. Ceccarelli.
2003. QTLs for agronomic traits in the Mediterranean environment
identified in recombinant inbred lines of the cross \'Arta\' × H.
spontaneum 41-1. Theor. Appl. Genet. 107:1215-1225.

Cakir, M., D. Poulsen, N.W. Galwey, G.A. Ablett, K.J. Chalmers, G.J.
Platz, R.C.M. Lance, J.F. Panozzo, B.J. Read, D.B. Moody, A.R. Barr, P.
Johnston, C.D. Li, W. J.R. Boyd, C R. Grime, R. Apples, M.G.K. Jones and
P. Langridge. 2003. Mapping and QTL analysis of the barley population
Tallon × Kaputar. Aust. J. Agr.Res. 54:1155-1162.

Clancy, J.A., F. Han and S.E. Ullrich. 2003. Comparative mapping of
beta-amylase activity QTLs among three barley crosses. Crop Sci.
43:1043-1052.

Dahleen, L.S., R. Horsley, B.J. Steffenson, P.B. Schwarz, A. Mesfin and
J.D. Franckowiak. 2003. Identification of QTLs associated with Fusarium
head blight resistance in Zhedar 2 barley. Theor. Appl. Genet.
108:95-104.

Han, F., S.E. Ullrich, I. Romagosa, J.A. Clancy, J.A. Froseth and D.M.
Wesenberg. 2003. Quantitative genetic analysis of acid detergent fibre
content in barley grain. J. Cereal Sci. 38:167-172.

Karakousis, A., A.R. Barr, J.M. Kretschmer, S. Manning, S.J. Logue, S.J.
Logue, C.D. Li, R.C.M. Lance and P. Langridge. 2003. Mapping and QTL
analysis of the barley population Galleon × Haruna Nij. Aust. J. Agr.
Res. 54:1131-1135.

Laurie, D.A., N. Pratchett, J.H. Bezant and J. W. Snape. 1995. RFLP
mapping of 5 major genes and 8 quantitative trait loci controlling
flowering time in a winter × spring barley (*Hordeum vulgare* L.) cross.
Genome 38:575-585

Long, N.R., S.P. Jefferies, A. Karakousis, J.M. Kretschmer, C. Hunt., P.
Lim, P.J. Eckermann and A. R. Barr. 2003. Mapping and QTL analysis of
the barley population Mundah × Keel. Aust. J. Agr. Res. 54:1163-1171.

Madsen, L.H., N.C. Collins, M. Rakwalska, G. Backes, N. Sandal, L.
Krusell, J. Jensen, E. H. Waterman, A. Jahoor, M. Ayliffe, A.J. Pryor,
P. Langridge, P. Schulze-Lefert and J. Stougaard. 2003. Barley disease
resistance gene analogs of the NBS-LRR class: identification and
mapping. Mol. Gen. Genet. 269:150-161.

Mickelson, S., D. See, F.D. Meyer, J.P. Garner, C.R. Foste, T.K. Blake
and A.M. Fischer. 2003. Mapping of QTL associated with nitrogen storage
and remobilization in barley (*Hordeum vulgare* L.) leaves. J. Exp. Bot.
54:801-812.

Pillen, K., A. Zacharias and J. Léon. 2003. Advanced backcross QTL
analysis in barley (*Hordeum vulgare* L.). Theor. Appl. Genet.
107:340-352.

Read, B.J., H. Raman, G. McMichael, K.J. Chalmers, G.A. Ablett, G.J.
Platz, R. Raman, R. K.Genger, J.R. Boyd, C.D. Li, C.R. Grime, R.F. Park,
H. Wallwork, R. Prangnell and R.C.M. Lance. 2003. Mapping and QTL
analysis of the barley population Sloop × Halcyon. Aust. J. Agr. Res.
54:1145-1153.

Teulat, B., N. Zoumarou-Wallis, B. Rotter, M. Ben Salem and D. This.
2003. QTL for relative water content in field-grown barley and their
stability across Mediterranean environments. Theor. Appl. Genet.
108:181-188.

Vaz Patto, M., D. Rubiales, A. Martín, P. Hernàndez, P. Lindhout, R.E.
Niks and P. Stam. 2003. QTL mapping provides evidence for lack of
association of the avoidance of leaf rust in *Hordeum chilense* with
stomata density. Theor. Appl. Genet. 106:1283-1292.

**Coordinator's report: Chromosome 2H (2)**

J.D. Franckowiak

Department of Plant Sciences

North Dakota State University

Fargo, ND 58105, U.S.A.

Dahleen *et al*., 2003 and Mesfin *et al*. 2003 published papers on the
inheritance of resistance to Fusarium head blight (FHB), incited
primarily by *Fusarium graminearum* Schwabe), in crosses between two-
and six-rowed barley. Quantitative trait loci (QTL) for FHB resistance
were again reported to occur in chromosome 2H. The reports identified
three QTLs for FHB resistance and two coincident QTLs for deoxynivalenol
(DON) accumulation in chromosome 2H. One QTL is near the *vrs1*
(six-rowed spike 1) locus and another is near the *Eam6* (early maturity
6) locus. The late two-rowed parents had QTLs for FHB resistance. Mesfin
*et al*., 2003 reported that the largest heading date effect associated
with the *Eam6* gene was observed in a fall greenhouse nursery.

Krasheninnik and Franckowiak, 2003 studied that FHB resistance in the
Harrington/Morex (HM) doubled-haploid population and found the largest
QTL for FHB resistance in chromosome 2H. The map developed for the HM
population by Marquez-Cedillo *et al*., 2001 was used in the analysis of
data. A QTL for early heading in China, a short-day environment, was at
the same position in chromosome 2H as the heading date QTL reported for
long-day response. This suggests that *Eam6* influences heading date in
both long- and short-day environments. A preliminary report by
Franckowiak *et al*., 2003 suggests that *Eam6* is ineffective in New
Zealand where days are slightly longer than 12 hours at planting.

Tanno *et al*., 2002 used molecular marker cMWG699, which is very close
(01. cM) to the *vrs1* locus, to study the origins of cultivated
six-rowed barley. Based on marker differences, they divided six-rowed
barleys into two distinct groups, types I and II. Type I is widely
distributed while Type II is found only in the Mediterranean region.
Since both types exist among two-rowed barley cultivars, six-rowed
barley probably originated from at least two independent mutations at
the *vrs1* locus.

Ayoub *et al*., 2002 studied kernel size and shape in the HM
doubled-haploid population. They found a large QTL for kernel size
associated with the *vrs1* locus. The two-rowed cultivar, Harrington,
had larger kernels than the six-rowed cultivar, Morex. Similar results
on 1000-kernel weights were reported by Hori *et al*., 2003 using
another two- by six-rowed population of F~9~ recombinant inbred lines.
These results with previous studies that found pleiotropic effects of
*vrs1* alleles on kernel size.

Weerasena *et al*., 2003 reported on the conversion of amplified
fragment length polymorphism (AFLP) marker P13M40 to a co-dominant
marker for *Rph15* (reaction to *Puccinia hordei* 15) locus in
chromosome 2HS. This gene conferred resistance all expect one isolate in
a collection of over 350 *P. hordei* isolates (Fetch *et al*., 1998).
The leaf rust resistance gene *Rph15.ad* was shown to be an allele of
the gene *Rph16.ae*, which was identified by Ivandic *et al*., 1998 in
wild barley (*Hordeum vulgare* ssp. *spontaneum*).

Backes *et al*., 2003 identified a QTL for resistance to powdery mildew
(*Blumeria graminis* f. sp. *hordei*) in chromosome 2HS and a QTL for
leaf rust (*Puccinia* *hordei*) resistance in chromosome 2HL. The study
was conducted using the progeny of a cross between 'Vada' and wild
barley accession 1B-87 from Israel.

Decousset *et al*., 2000 reported on the development of sequence tagged
site (STS) primer pairs for the *Ppd-H1* or *Eam1* locus in chromosome
2HS. Plants with the *Eam1* gene are very early when grown under
long-day conditions (Tohno-oka *et al*., 2000).

Canci *et al*., 2003 identified a minor QTL for kernel discoloration in
chromosome 2H. However, two major QTLs for kernel color were found in
chromosome 6H and one of these was coincident with a major QTL for high
grain protein from 'Chevron'.

Li *et al*., 2003 mapped 127 new microsatellite markers in barley. One
of the four large clusters of makers that they found was in chromosome
2H.

Arru *et al*., 2003 mapped a QTL for resistance to leaf stripe
(*Pyrenophora graminea*) in chromosome 2H of 'Steptoe'. The QTL is at a
different position in chromosome 2H than the *Rdg1* locus, which also
confers resistance to leaf stripe of barley.

**References:**

Arru, L., E. Francia, and N. Pechioni. 2003. Isolate-specific QTLs of
resistance to leaf stripe (*Pyrenophora graminea*) in the 'Steptoe' X
'Morex" spring barley cross. Theor. Appl. Genet. 106:668-675.

Ayoub, M. S.J. Symons, M.J. Edney, and D.E. Mather. 2002. QTLs affecting
kernel size and shape in a two-rowed by six-rowed barley cross. Theor.
App. Genet. 105:237-247.

Backes, G., L.H. Madsen, H. Jaiser, J. Stougaard, M. Herz, V. Mohler,
and A. Jahoor. 2003. Localisation of genes for resistance against
*Blumeria graminis* f. sp. *hordei* and *Puccinia* *graminis* in a cross
between a barley cultivar and a wild barley (*Hordeum vulgare* ssp.
*spontaneum*) line. Theor. Appl. Genet. 106:353-362.

Canci, P.C., L.M. Nduulu, R. Dill-Macky, G.J. Muehlbauer, D.C.
Rasmusson, and K.P. Smith. 2003. Genetic relationship between kernel
discoloration and grain protein concentation in barley. Crop Sci.
43:1671-1679.

Dahleen, L.S., H.A. Agrama, R.D. Horsley, B.J. Steffenson, P.B. Schwarz,
A. Mesfin, and J.D. Franckowiak. 2003. Identification of QTLs associated
with Fusarium head blight resistance in Zhedar 2. Theor. Appl. Genet.
108:95-104.

Decousset, L., S. Griffiths, R.P. Dunford, N. Pratchett, and D.A.
Laurie. 2000. Development of STS markers closely linked to the *Ppd-H1*
photoperiod response gene in barley (*Hordeum vulgare* L.). Theor. Appl.
Genet. 101:1202-1206.

Fetch, T,G. Jr., B.J. Steffenson, and Y. Jin. 1998. Worldwide virulence
of *Puccinia hordei* on barley. Phytopathology 88:528.

Franckowiak, J.D., G. Yu, and N. Krasheninnik. 2003. Genetic control of
photoperiod responses in spring barley. p. 98. *In* Proc. 3rd Canadian
Barley Symposium, 19 and 20 June, 2003, Red Deer, Alberta, Canada.

Hori, K., T. Kobayashi, A. Shimizu, K. Sato, K. Takeda, and S. Kawasaki.
2003. Efficient construction of high-density linkage map and its
application to QTL analysis in barley. Theor. Appl. Genet. 107:806-813.

Ivandic, V., U. Walther, and A. Graner. 1998. Molecular mapping of a new
gene in wild barley conferring complete resistance to leaf rust
(*Puccinia hordei* Otth). Theor. Appl. Genet. 97:1235-1239.

Krasheninnik, N.N., and J.D. Franckowiak. 2003. Identification of QTLs
in the Harrington/Morex barley population for FHB reaction, maturity,
and plant height. p. 260-263. *In* S. Canty, J. Lewis, and R.W. Ward
(eds.) Proc. National Fusarium Head Blight Forum, 2003 Dec 13-15;
Bloomington, MN. Michigan State University, East Lansing.

Li, J.Z., T.G. Sjakste, M.S. Röder, and M.W. Ganal. 2003. Development
and genetic mapping of 127 new microsatellite markers in barley. Theor.
Appl. Genet. 107:1021-1027.

Marquez_Cedillo, L.A., P.M. Hayes, A. Kleinhofs, W.G. Legge, B.G.
Rossnagel, K. Sato, S.E. Ullrich, and D. M. Wesenberg. 2001. QTL
analysis of agronomic traits in barley based on the doubled haploid
progeny of two elite North American varieties representing different
germplasm groups. Theor. Appl. Genet. 103:625-637.

Mesfin, A. K.P. Smith, R. Dill-Macky, C.K. Evans, R. Waugh, C.D. Gustus,
and G.J. Muehlbauer. 2003. Quantitative trait loci for Fusarium head
blight resistance in barley detected in a two-rowed by six-rowed
population. Crop Sci. 43:307-318.

Tanno, K., S. Taketa, K. Takeda, and T. Komatsuda. 2002. A DNA marker
closely linked to the *vrs1* locus (row-type gene) indicates multiple
origins of six-rowed cultivated barley (*Hordeum vulgare* L.). Theor.
Appl. Genet. 104:54-60.

Tohno-oka, T., M. Ishit, R. Kanatani, H. Takahashi, and K. Takeda. 2000.
Genetic analysis of photoperiodic response of barley in different
daylength conditions. p. 239-241. *In* S. Logue (ed.) Proc. Eight Int.
Barley Genet. Symp., Volume III. Adelaide University, Glen Osmond, South
Australia.

Weerasena, J.S., B.J. Steffenson, and A.B. Falk. 2003. Conversion of an
amplified fragment length polymorphism marker into a co-dominant marker
in mapping of the *Rph15* gene conferring resistance to barley leaf
rust, *Puccinia hordei* Otth. Theor. Appl. Genet., in press.

**Coordinators Report: Barley Chromosome 5H (7)**

**George Fedak**

Eastern Cereal & Oilseed Research Centre

Agriculture & Agri-Food Canada

Ottawa, Ontario, K1A 0C6

The seed dormancy loci SD1 and SD2 of Steptoe barley have previously
been mapped to chromosome 7 (5H) (Han *et al*., 1996). *SD2* had been
mapped to a 8cM interval between markers ABC 309 and MWG851 at the
distal end of the long arm (Ullrich *et al.*, 1996). This locus was
subsequently fine mapped to a 0.8cM interval using a substitution
mapping approach. The flanking markers were MWG 851D and MWG 851B (Gao
*et al.*, 2003). There was probably another dormancy QTL in the ABG
496 - MWG 851C interval of 5H that reduced the dormancy effect of *SD2.*

The locus Ba YMV/Ba YMV-2, that provides resistance to the two strains
of barley yellow mosaic virus from the barley variety Chikurin Ibaraki
was mapped to chromosome 5H. Three SSR markers, Bmac 0306, Bmac 0163 and
Bmac 0113 cosegrated with the resistance locus. It was concluded that
the resistance locus is included in a 4.3cM interval spanned by the
three markers (Werner *et al*., 2003).

It is interesting to note that the barley yellow mosaic resistance locus
rym3 has previously been mapped to chromosome 5H (Saeki *et al.,* 1999).
The resistance locus was obtained from barley line Ea52. The latter is a
gamma ray induced mutant of the variety Chikurin Ibaraki.

**References:**

Gao, W., J.A. Clancy, F. Han, D. Prada, A. Kleinhofs and S.E. Ullrich.
2003. Molecular dissection of a dormancy QTL region near the chromosome
7 (5H) L Telomere in barley. Theor. Appl. Genet. 107:552-559.

Han, F., S.E. Ullrich, J.A. Clancy and I. Ramagosa, 1999. Inheritance
and fine mapping of a major barley seed dormancy QTL. Plant Sci.
143:113-118.

Saeki, K., C. Miyazaki, N. Hirota, A. Saito, K. Ito and T. Konishi,
1999. RFLP mapping of BaYMV resistance gene *rym3* in barley (*Hordeum
vulgare*). Theor. Appl. Genet. 99:727-732.

Ullrich, S.E., F. Han, T.K. Blake, L.E. Oberthur, W.E. Dyer and J.A.
Clancy, 1996. Seed dormancy in barley. Genetic resolution and
relationships to other traits. pp:157-163. *In*: Noda, K. and Mares,
D.J. (eds.) Preharvest sprouting in cereals 1995. Center for Academic
Societies, Osaka, Japan, Proc.7th International Symposium on Pre-Harvest
Sprouting in Cereals held at Abashiri, Hokkaido, Japan.

Werner, K., W. Friedt, E. Laubach, R. Waugh and F. Ordon. 2003.
Dissection of resistance to soil-borne yellow-mosaic-inducing viruses of
barley (BaMMV, BaYMV, BaYMV-2) in a complex breeders=s cross by means of
SSRs and simultaneous mapping of BaYMV/BaYMV-2 resistance of var.
'Chikurin Ibaraki 1'. Theor Appl. Genet. 106:1425-1432.

**Coordinator's report: Chromosome 7H**

**Lynn S. Dahleen**

USDA-Agricultural Research Service

Fargo, ND 58105, USA

Three studies examined polymorphisms among various genotype collections.
Russell *et al.*, 2003 sampled landraces from Syria and Jordan and
tested polymorphism with 21 SSRs. One of the chromosome 7H SSRs tested
showed three alleles while the other SSR locus had 31 different alleles
in the 125 landrace accessions genotyped. Sjakste *et al*., 2003
examined microsatellite allele inheritance in the 37 European ancestors
of seven Latvian varieties. The 14 SSRs tested from chromosome 7H two to
seven alleles, and an average polymorphism information content (PIC)
value of 0.62, a bit above the overall PIC average of 0.57. Allelic
pedigrees were constructed to trace the genetic route of each current
allele back to the specific ancestral source. Lund *et al*., 2003 used
SSRs to evaluate potential duplicate groups in gene bank collections.
The 35 primer pairs tested, six from chromosome 7H, identified 22
homogeneous groups among the 36 groups studied, providing a rapid method
for identifying duplicates.

Pillen *et al*., 2003 conducted a QTL analysis of a BC~2~F~2~ population
between an *H. v. spontaneum* and the recurrent spring barley Apex. QTLs
for heading date, harvest index, malt tenderness, yield, height,
thousand-grain weight, water absorption, and above ground biomass were
located on chromosome 7H. Many of the favorable alleles for these
important traits were from *H. v. spontaneum*. Matus *et al*., 2003
developed recombinant chromosome substitution lines by backcrossing an
*H. vulgare* subsp. *spontaneum* accession to Harrington barley. They
then used 47 SSR markers to determine the percentage of *spontaneum*
introgressed into the lines. The average length of the donor segment in
chromosome 7H was 39.0 cM, with segregation distorted towards the
donor's DNA. Baum *et al*., 2003 tested recombinant inbred lines from
another *H. v. spontaneum* cross, with Arta. They developed a linkage
map on 189 markers, including 24 on chromosome 7H. QTLs located on this
chromosome included those for grain yield, 1000-kernel weight, days to
heading, plant height, beta-glucan content, biological yield, and cold
damage. Only the QTL for cold damage was significant in more than one
environment. SSRs were used with genomic in situ hybridization (GISH) to
evaluate wheat x barley backcross-derived lines (Malysheva *et al*.,
2003). One BC~1~ plant contained only two small 7H fragments which were
not transmitted to the progeny. The other plant contained the complete
chromosome 7H. Only the end of the short arm was detected in BC~2~
progeny. Similar patterns of elimination were observed for other
chromosomes.

Several groups reported on the development of additional markers. Li *et
al*., 2003 developed 127 new SSR markers from genomic clones. Nine of
the SSRs were located on chromosome 7H. Thiel *et al*., 2003 developed
76 new SSR markers from EST database information; seven were on
chromosome 7H. Kota *et al*., 2003 also used EST collections to identify
single nucleotide polymorphisms (SNPs) in barley. Of the 28 SNPs mapped
in this study, three were on chromosome 7H. Another type of marker,
based on NBS-LRR class resistance gene analogs (RGAs), was developed and
mapped by Madsen *et al*., 2003. Three of these RGAs mapped to
chromosome 7H.

Placement of morphological markers on the molecular maps continued.
Pozzi *et al*., 2003 mapped 29 developmental mutants using RFLP-AFLP
techniques. Both the *lks2* gene for short awns and the *sld4* gene for
slender dwarf 4 were placed on the Proctor x Nudinka AFLP map of
chromosome 7H. Dahleen *et al*., 2003 identified linkages between SSR
markers on chromosome 7H and the *lks.o* and *bra-a.001* morphological
genes. Kikuchi *et al.*, 2003 fine-mapped the naked caryopsis gene,
*nud*, using bulked segregant analysis and AFLP markers. The *nud* locus
was mapped within a 1.5 cM region and cosegregated with two AFLP
markers. Their data show that *nud* is further from the centromere than
previously reported.

Gene mapping reports also included those involved in disease response.
Chen *et al*., 2003 compared genomic locations of rice and barley QTLs
for resistance to rice blast. The 12 QTLs in barley included three on
chromosome 7H. One of these loci was syntenic to a locus on rice
chromosome 8. One of the QTL maps for Fusarium head blight (FHB),
developed by Mesfin *et al*., 2003, identified several FHB QTLs, but
only detected one small QTL for resistance in the greenhouse on
chromosome 7H. Rostoks *et al.*, 2003 isolated and mapped genes
homologous to maize hypersensitive-induced reaction (*HIR*) genes.
*Hv-hir4* was located on the short arm of chromosome 7H in bin 03. This
genes was similar to the other three *HIR* genes at the amino acid
level, but not at the sequence level.

QTL analysis of recombinant inbred lines from a 2-rowed by 6-rowed
spring barley cross was conducted using a map developed using the high
efficiency genome scanning system (Hori *et al*., 2003). This system
allowed map construction in six months, and QTLs for plant height and
spike exertion were located on chromosome 7H. Teulat *et al*., 2003
located QTLs for relative water content in field-grown barley. One QTL
representing main and QTL x E effects and one QTL that was detected only
in one environment were located on chromosome 7H. Clancy *et al*., 2003
conducted comparative mapping of beta-amylase activity QTLs among the
three mapping populations from the North American Barley Genome Project.
Both populations involving Morex showed coincident QTLs for beta amylase
and diastatic power on chromosome 7H. This region contains several other
QTL for malting quality. QTLs for kernel discoloration and grain protein
content were located by Canci *et al*., 2003. Two QTLs for kernel
discoloration located on chromosome 7H were detected in a single
environment, explaining 6.4-10.1 % of the variation for this trait. No
regions on this chromosome were associated with grain protein content.

**References:**

Baum, M., S. Grando, G. Backes, A. Jahoor, A. Sabbagh, and S.
Ceccarelli. 2003. QTLs for agronomic traits in the Mediterranean
environment identified in recombinant inbred lines of the cross 'Arta' x
*H. spontaneum* 41-1. Theor. Appl. Genet. 107:1215-1225.

Canci, P.C., L.M. Nduulu, R. Dill-Macky, G.M. Muehlbauer, D.C.
Rasmusson, and K.P. Smith. 2003. Genetic relationships between kernel
discoloration and grain protein concentration in barley. Crop Sci.
43:1671-1679.

Chen, H., S. Wang, Y. Xing, C. Xu, P.M. Hayes, and Q. Zhang. 2003.
Comparative analyses of genomic locations and race specificities for
quantitative resistance to *Pyricularia grisea* in rice and barley.
Proc. Natl. Acad. Sci. USA 100:2544-2549.

Clancy, J.A., F. Hans, S.E. Ulrich, and the North American Barley Genome
Project. 2003. Comparative mapping of B-amylase activity QTLs among
three barley crosses. Crop Sci. 43:1043-1052.

Dahleen, L.S., J.D. Franckowiak, and L.J. Vander Wal. 2003. Exposing
students and teachers to plant molecular genetics with short-term barley
gene mapping projects. J. Nat. Resour. Life Sci. Educ. 32:61-64.

Hori, K., R. Kobayashi, A. Shimizu, K. Sato, K. Takeda, and S. Kawasaki.
2003. Efficient construction of high-density linkage map and its
application to QTL analysis in barley. Theor. Appl. Genet. 107-806-813.

Kikuchi, S., S. Taketa, M. Ichii, and S. Kawasaki. 2003. Efficient fine
mapping of the naked caryopsis gene (*nud*) by HEGS (High Efficiency
Genome Scanning) /AFLP in barley. Theor. Appl. Genet. 108:73-78.

Kota, R., S. Rudd, A. Facius, G. Kolesov, T. Thiel, H. Zhang, N. Stein,
K. Mayer, and A. Graner. 2003. Snipping polymorphisms from large EST
collections in barley (*Hordeum vulgare* L.). Mol. Gen. Genomics
270:24-33.

Li, J.Z., T.G. Sjakste, M.S. Roder, and M.W. Ganal. 2003. Development
and genetic mapping of 127 new microsatellite markers in barley. Theor.
Appl. Genet. 107:1021-1027.

Lund, B., R. Oritz, I.M. Skovgaard, R. Waugh, and S.B. Andersen. 2003.
Analysis of potential duplicates in barley gene bank collections using
re-sampling of microsatellite data. Theor. Appl. Genet. 106:1129-1138.

Madsen, L.H., N.C. Collins, M. Rakwalska, G. Backes, N. Sandal., L.
Krusell, J. Jensen, E.H. Waterman, A. Jahoor, M. Ayliffe, A.J. Pryor, P.
Langridge, P. Schulze-Lefert, and J. Stougaard. 2003. Barley disease
resistance gene analogs of the NBS-LRR class: identification and
mapping. Mol. Gen. Genomics 269:150-161.

Malysheva, L., T. Sjakste, F. Matzk, M. Roder, and M. Ganal. 2003.
Molecular cytogenetic analysis of wheat-barley hybrids using genomic in
situ hybridization and barley microsatellite markers. Genome 46:413-322.

Matus, I., A. Corey, T. Filichkin, P.M. Hayes, M.I. Vales, J. Kling, O.
Riera-Lizarazu, K. Sato, W. Powell, and R. Waugh. 2003. Development and
characterization of recombinant chromosome substitution lines (RCSLs)
using *Hordeum vulgare* subsp. *spontaneum* as a source of donor alleles
in a *Hordeum vulgare* subsp. *vulgare* background. Genome 46:1010-1023.

Mesfin, A., K.P. Smith, R. Dill-Macky, C.K. Evans, R. Waugh, C.D.
Gustus, and G. Muehlbauer. 2003. Quantitative trait loci for Fusarium
head blight resistance in barley detected in a two-rowed by six-rowed
population. Crop Sci. 43: 307-318.

Pillen, K., A. Zacharias, and J. Leon. 2003. Advanced backcross QTL
analysis in barley (*Hordeum vulgare* L.). Theor. Appl. Genet.
107:340-352.

Pozzi, C., D. di Pietro, G. Halas, C. Roig, and F. Salamini. 2003.
Integration of a barley (*Hordeum vulgare*) molecular linkage map with
the positions of genetic loci hosting 29 developmental mutants. Heredity
90:390-396.

Rostoks, N., D. Schmierer, D. Kudrna, and A. Kleinhofs. 2003. Barley
putative hypersensitive induced reaction genes: genetic mapping,
sequence analysis and differential expression in disease lesion mimic
mutants. Theor. Appl. Genet. 107:1094-1101.

Russell, J.R., A. Booth, J.D. Fuller, M. Baum, S. Ceccarelli, S. Grando,
and W. Powell. 2003. Patterns of polymorphism detected in the
chloroplast and nuclear genomes of barley landraces sampled from Syria
and Jordan. Theor. Appl. Genet. 107:413-421.

Sjakste, T.G., I. Rashal, and M.S. Roder. 2003. Inheritance of
microsatellite alleles in pedigrees of Latvian barley varieties and
related European ancestors. Theor. Appl. Genet. 106:539-549.

Teulat, B., N. Zoumarou-Wallis, B. Rotter, M. Ben Salem, H. Bahri, and
D. This. 2003. QTL for relative water content in field-grown barley and
their stability across Mediterranean environments. Theor. Appl. Genet.
108:181-188.

Thiel, T., W. Michalek, R.K. Varshney, and A. Graner. 2003. Exploiting
EST databases for the development and characterization of gene-derived
SSR markers in barley (*Hordeum vulgare* L.). Theor. Appl. Genet.
106:411-422.

**Integrating Molecular and Morphological/Physiological Marker Maps**

**A. Kleinhofs**

Dept. Crop and Soil Sciences and

School of Molecular Biosciences

Washington State University

Pullman, WA 99164-6420, USA

During the past year I have attempted to integrate the Morphological
/Physiological/ Disease resistance markers into the molecular Bin map.
The results were sent to Grain Genes to produce an integrated map. My
goal is to produce an interactive map that would incorporate pictures of
markers that are "photogenic", decriptions of markers, high resolution
maps where available, BAC clone addresses, etc. The results can be
viewed at <http://ceres.plbr.cornell.edu/cgi-bin/gbrowse>. This is
obviously a work in progress, but to date it is not very viewer friendly
or easy to navigate through, but the information is there. For those who
would prefer the information in a less cumbersome form I reproduce it
her, minus the pictures and high resolution maps, of course.

Please advise me if you have additions or corrections to this
information.

> **Bin Assignments for Morphological Map Markers and closest molecular
> marker**
>
> Chr.1(7H)

BIN1 [Rpg1]{.mark} RSB228 Brueggeman et al., PNAS 99:9328, '02

> Run1
>
> [Rdg2a]{.mark} MWG851A Bulgarelli et al., TAG 108:1401-1408 ,'04
>
> [Rrs2]{.mark} MWG555A Schweizer et al., TAG 90:920, '95
>
> mlt
>
> [brh1]{.mark} MWG2074B Li et al., 8th IBGS 3:72, '00
>
> BIN2 Est5 iEst5 Kleinhofs et al., TAG 86:705, '93
>
> [wax]{.mark} Wax Kleinhofs BGN32:152, '02
>
> [gsh3]{.mark} His3A Kleinhofs BGN32:152, '02
>
> BIN3 [fch5]{.mark} ABC167A Kleinhofs BGN32:152, '02
>
> [Rcs5]{.mark} KAJ185 Johnson & Kleinhofs, unpublished
>
> yvs2
>
> [cer-ze]{.mark} ABG380 Kleinhofs BGN27:105, '96
>
> BIN4 wnd
>
> [Lga]{.mark} BE193581 Johnson & Kleinhofs, unpublished

abo7

> BIN5 ant1
>
> [nar3]{.mark} MWG836 Kleinhofs BGN32:152, '02
>
> ert-m
>
> ert-a
>
> BIN6 ert-d
>
> fch8
>
> fst3
>
> cer-f
>
> dsp1
>
> msg14
>
> BIN7 msg10
>
> [rsm1]{.mark} BC455 Edwards & Steffenson, Phytopath. 86:184,'96
>
> sex6
>
> seg5
>
> seg2
>
> [pmr]{.mark} ABC308 Kleinhofs BGN27:105, '96
>
> [mo6b]{.mark} Hsp17 Soule et al., J Her. 91:483, '00
>
> [nud]{.mark} CDO673 Heun et al., Genome 34:437, '91
>
> [fch4]{.mark} MWG003 Kleinhofs BGN27:105, '96
>
> BIN8 [Amy2]{.mark} Amy2 Kleinhofs et al., TAG 86:705, '93
>
> [lks2]{.mark} WG380B Costa et al., TAG 103:415, '01
>
> [Rpt4]{.mark} Psr117D Williams et al., TAG 99:323, '99
>
> ubs4
>
> blx2
>
> BIN9 lbi3
>
> xnt4
>
> [lpa2]{.mark} ? Larson et al., TAG 97:141, '98
>
> msg50
>
> Rym2
>
> seg4
>
> BIN10 [Xnt1]{.mark} BF626025 Hansson et al., PNAS 96:1744, '99
>
> [xan-h]{.mark} BF626025 Hansson et al., PNAS 96:1744, '99
>
> BIN11 [Rph3]{.mark} Tha2 Toojinda et al., TAG 101:580, '00
>
> BIN12 Mlf
>
> xnt9
>
> seg1
>
> msg23
>
> BIN13 none
>
> BIN14 none
>
> Chr.2(2H)
>
> BIN1 sbk
>
> BIN2 none
>
> BIN3 [gsh6]{.mark} MWG878A Kleinhofs BGN32:152, '02
>
> gsh1
>
> gsh8
>
> BIN4 Eam1
>
> [Ppd-H1]{.mark} MWG858 Laurie et al., Heredity 72:619, '94
>
> sld2
>
> rtt
>
> flo-c
>
> sld4
>
> BIN5 fch15
>
> brc1
>
> com2
>
> BIN6 msg9
>
> abo2
>
> [rph16]{.mark} MWG874 Drescher et al., 8thIBGS II:95, '00
>
> BIN7 [yst4]{.mark} CDO537 Kleinhofs BGN32:152, '02
>
> [Az94]{.mark} CDO537 Kleinhofs BGN32:152, '02
>
> [gai]{.mark} MWG2058 Börner et al., TAG 99:670, '99
>
> msg33
>
> msg3
>
> fch1
>
> BIN8 [Eam6]{.mark} ABC167b Tohno-oka et al., 8thIBGS III:239, '00
>
> gsh5
>
> msg2
>
> [eog]{.mark} ABC451 Kleinhofs BGN27:105, '96
>
> abr
>
> cer-n
>
> BIN9 Gth
>
> hcm1
>
> wst4
>
> [vrs1]{.mark} MWG699 Komatsuda et al., Genome 42:248, '00
>
> BIN10 cer-g
>
> Lks1
>
> mtt4
>
> Pre2
>
> msg27
>
> ant2
>
> BIN11 [Rha2]{.mark} AWBMA21 Kretschmer et al., TAG 94:1060, '97
>
> [Rar1]{.mark} AW983293B Freialdenhoven et al., Plt. Cell 6:983, '94
>
> fol-a
>
> [gal]{.mark} MWG581A Börner et al., TAG 99:670, '99
>
> fch14
>
> Pau
>
> BIN12 Pvc
>
> BIN13 [lig]{.mark} BCD266 Pratchett & Laurie Hereditas 120:35, '94
>
> [nar4]{.mark} Gln2 Kleinhofs BGN27:105, '96
>
> [Zeo1]{.mark} cnx1 Costa et al., TAG 103:415, '01
>
> [lpa1]{.mark} ABC157 Larson et al., TAG 97:141, '98
>
> BIN14 none
>
> BIN15 [gpa]{.mark} CDO036 Kleinhofs BGN27:105, '96
>
> [wst7]{.mark} MWG949A Costa et al., TAG 103:415, '01
>
> [MlLa]{.mark} Ris16 Giese et al., TAG 85:897, '93
>
> trp
>
> Chr. 3(3H)
>
> BIN1 [Rph5]{.mark}
>
> [Rph6]{.mark}
>
> [Rph7]{.mark} MWG848 Brunner et al., TAG 101:783, '00
>
> BIN2 ant17
>
> sld5
>
> [mo7a]{.mark} ABC171A Soule et al., J. hered. 91:483, '00
>
> brh8
>
> BIN3 xnt6
>
> BIN4 [btr1]{.mark}
>
> [btr2]{.mark}
>
> lzd
>
> BIN5 [alm]{.mark} ABG471 Kleinhofs BGN27:105, '96
>
> abo9
>
> sca
>
> yst2
>
> dsp10
>
> BIN6 [Rrs1]{.mark} Graner et al., TAG 93: 421 ´96
>
> [Rrs.B87]{.mark} BCD828 Williams et al., Plant Breed. 120:301, '01
>
> [Rh/Pt]{.mark} ABG396 Smilde et al., 8th IBGS 2:178, '00
>
> abo6
>
> xnt3
>
> msg5
>
> ari-a
>
> yst1
>
> zeb1
>
> ert-c
>
> ert-ii
>
> cer-zd
>
> [Ryd2]{.mark} WG889B Collins et al., TAG 92:858, '96
>
> BIN7 uzu
>
> cer-r
>
> BIN8 wst6
>
> cer-zn
>
> sld1
>
> BIN9 wst1
>
> BIN10 vrs4
>
> lnt1
>
> gsh2
>
> BIN11 als
>
> [sdw1]{.mark} PSR170 Laurie et al., Plant Breed. 111:198, '93
>
> BIN12 sdw2
>
> BIN13 [Pub]{.mark} ABG389 Kleinhofs et al., TAG 86:705, '93´
>
> BIN14 cur2
>
> BIN15 Rph10
>
> fch2
>
> BIN16 eam10
>
> Est1/2/3
>
> [rym4]{.mark} MWG010 Graner & Bauer TAG 86:689, '93
>
> [rym5]{.mark} MWG838 Graner et al., TAG 98:285, '99
>
> Est4
>
> ant28

Chr.4(4H)

> BIN1 none
>
> BIN2 fch9
>
> sln
>
> BIN3 int-c
>
> Zeo3
>
> [Dwf2]{.mark} Ole1 Ivandic et al., TAG 98:728, '99
>
> Ynd
>
> glo-a
>
> rym1 ? Konishi et al., TAG 94:871, '97
>
> BIN4 [Kap]{.mark} X83518 Muller et al., Nature 374:727, '95
>
> lbi2
>
> zeb2
>
> lgn3
>
> BIN5 lgn4
>
> lks5
>
> eam9
>
> msg24
>
> BIN6 glf1
>
> [rym11]{.mark} MWG2134 Bauer et al., TAG 95:1263, '97
>
> [Mlg]{.mark} MWG032 Kurth et al., TAG 102:53, '01
>
> cer-zg
>
> brh2
>
> BIN7 glf3
>
> frp
>
> min1
>
> blx4
>
> sid
>
> blx3
>
> BIN8 blx1
>
> BIN9 ert1
>
> BIN10 [mlo]{.mark} P93766 Bueschges et al., Cell 88:695, '97
>
> BIN11 none
>
> BIN12 [Hsh]{.mark} HVM067 Costa et al., TAG 103:415, '01
>
> Hln
>
> sgh1
>
> yhd1
>
> BIN13 [Bmy1]{.mark} pcbC51 Kleinhofs et al., TAG 86:705, '93
>
> [rym8]{.mark} MWG2307 Bauer et al., TAG 95:1263, '97
>
> [rym9]{.mark} MWG517 Bauer et al., TAG 95:1263, '97
>
> Wsp3

Chr. 5(1H)

> BIN1 Rph4
>
> Mlra
>
> Cer-yy
>
> [Sex76]{.mark} Hor2 Netsvetaev BGN27:51, '97
>
> [Hor5]{.mark} Hor5 Kleinhofs et al., TAG 86:705, '93
>
> BIN2 [Hor2]{.mark} Hor2 Kleinhofs et al., TAG 86:705, '93
>
> [Rrs14]{.mark} Hor2 Garvin et al., Plant Breed. 119:193-196, '00
>
> [Mla6]{.mark} AJ302292 Halterman et al., Plt J. 25:335, '01
>
> BIN3 [Hor1]{.mark} Hor1 Kleinhofs et al., TAG 86:705, '93
>
> Rps4
>
> Mlk
>
> BIN4 Lys4
>
> BIN5, 6, 7. Mlnn; msg31; sls; msg4; fch3;
>
> BIN6 amo1
>
> BIN7 clh
>
> vrs3
>
> BIN8 fst2
>
> cer-zi
>
> cer-e
>
> ert-b
>
> MlGa
>
> msg1
>
> xnt7
>
> BIN9 [nec1]{.mark}
>
> BIN10 abo1
>
> Glb1
>
> BIN11 wst5
>
> cud2
>
> BIN12 rlv
>
> lel1
>
> BIN13 [Blp]{.mark} ABC261 Costa et al., TAG 103:415, '01
>
> BIN14 fch7
>
> trd
>
> eam8
>
> Chr. 6(6H)
>
> BIN1 [Nar1]{.mark} X57845 Kleinhofs et al., TAG 86:705, '93
>
> abo15
>
> BIN2 [nar8]{.mark} ABG378B Kleinhofs BGN27:105, '96
>
> nec3
>
> Rrs13
>
> BIN3 none
>
> BIN4 msg36
>
> BIN5 nec2
>
> ant21
>
> msg6
>
> eam7
>
> BIN6 [rob]{.mark} HVM031 Costa et al., TAG 103:415, '01
>
> sex1
>
> gsh4
>
> ant13
>
> [cul2]{.mark} Crg4(KFP128) Babb & Muehlbauer BGN31:28, '01
>
> fch11
>
> mtt5
>
> abo14
>
> BIN7 none
>
> BIN8 none
>
> BIN9 [Amy1]{.mark} JR115 Kleinhofs et al., TAG 86:705, '93
>
> [Nar7]{.mark} X60173 Warner et al., Genome 38:743, '95
>
> [Nir]{.mark} pCIB808 Kleinhofs et al., TAG 86:705, '93
>
> mul2
>
> cur3
>
> BIN10 lax-b
>
> raw5
>
> cur1
>
> BIN11 none
>
> BIN12 xnt5
>
> Aat2
>
> BIN13 [Rph11]{.mark} Acp3 Feuerstein et al., Plant breed. 104:318,
> '90\`
>
> lax-c
>
> BIN14 dsp9
>
> Chr. 7(5H)
>
> BIN1 abo12
>
> msg16
>
> ddt
>
> BIN2 dex1
>
> msg19
>
> nld
>
> fch6
>
> glo-b
>
> BIN3 [cud1]{.mark} ABG705A
>
> lys3
>
> fst1
>
> blf1
>
> vrs2
>
> BIN4 cer-zj
>
> cer-zp
>
> msg18
>
> wst2
>
> [Rph2]{.mark} ITS1 Borovkova et al., Genome 40:326, '97
>
> [lax-a]{.mark} PSR118 Laurie et al., TAG 93:81, '96
>
> com1
>
> ari-e
>
> ert-g
>
> ert-n
>
> BIN5 [rym3]{.mark} MWG028 Saeki et al., TAG 99:727, '99
>
> BIN6 none
>
> BIN7 none
>
> BIN8 none
>
> BIN9 [srh]{.mark} ksuA1B Kleinhofs et al., TAG 86:705, '93
>
> cer-I
>
> mtt2
>
> lys1
>
> cer-t
>
> dsk
>
> var1
>
> cer-w
>
> Eam5
>
> BIN10 raw1
>
> msg7
>
> BIN11 [Rph9/12]{.mark} ABG712
>
> [Sgh2]{.mark}
>
> lbi1
>
> Rha4
>
> raw2
>
> BIN12 none
>
> BIN13 [rpg4]{.mark} ARD5303 Druka et al., Mol.Gen.Genet. 264:283-290,
> '00
>
> [RpgQ]{.mark} ARD5304 Sun et al., Phytopath. 86:1299-1302, '96
>
> BIN14 var3

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Druka, A., D. Kudrna, F. Han, A. Kilian, B. Steffenson, D. Frisch, J.
Tomkins, R. Wing and A. Kleinhofs. 2000. Physical mapping of the barley
stem rust resistance gene rpg4. Mol. Gen. Genet. 264:283-290.

Edwards, M.C. and B.J. Steffenson. 1996. Genetics and mapping of barley
stripe mosaic virus resistance in barley. Phytopath. 86;184-187.

Feuerstein, U., A.H.D. Brown and J.J. Burdon. 1990. Linkage of rust
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Freialdenhoven, A., B. Scherag, K. Hollrichter, D.B. Collinge, H.
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Garvin, D.F., A.H.D. Brown, H. Raman and B.J. Read. 2000. Genetic
mapping of the barley *Rrs14* scald resistance gene with RLFP, isozyme
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Giese, H., A.G. Holm-Jensen, H.P. Jensen and J.Jensen. 1993.
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**Coordinator's report: Barley Genetic Stock Collection**

## A. Hang

USDA-ARS, National Small Grains

Germplasm Research Facility,

Aberdeen, Idaho 83210, USA

Over 100 barley genetic male sterile stocks were planted in the
greenhouse for seed increase. One hundred seventy-eight barley
translocation stocks were increased in the field. In collaboration with
Dr. Jerry Franckowiak, over 780 barley genetic stocks derived from
crossing with cultivar 'Bowman' were planted in the field at Aberdeen in
two-rowed single plot for seed increase and for agronomic evaluation.

Over one hundred-thirty barley genetic stocks were shipped to
researchers in 2003.

Thirty-eight nitrate reductase deficient mutants obtained from Dr. Andy
Kleinhofs (Table 1) were increased in the field in 2003.

Eleven globosum mutants from Dr. G. Fischbeck: glo-b.1 (W1), glo-b.2
(W2), glo-b.3 (W3), glo-b.4 (W4), glo-b.5 (W5), glo-d.1 (W6), glo-d.2
(W7), glo-d.3 (W8), glo-c (W9), glo-a (W10), and glo-e (W11) were
increased in the field in 2003.

Ten translocation lines from Dr. Andreas Houben: T1-al, T1-6ai, T1-7ao,
T2-5ah, T2-6aq, T2-7aj, T3-4ae, T3-7ax, T3-7aaa, and T5-6 ap, are being
increased in the greenhouse, 2004.

**Table 1. Nitrate reductase deficient mutants received from Dr. Andy
Kleinhofs.**

+-------+---------+--------+--------------+---------------------------+
| **En  | *       | **Line |              | **Function or Chemical    |
| try** | *Gene** | No.**  |              | Pathway**                 |
|       |         |        |              |                           |
|       | **S     |        |              |                           |
|       | ymbol** |        |              |                           |
+-------+---------+--------+--------------+---------------------------+
| 1     | nar1a   | AZ12   | 84-151       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 2     | nar1b   | AZ13   | Spillman     | NADH NR structural gene   |
|       |         |        | 6758         |                           |
+-------+---------+--------+--------------+---------------------------+
| 3     | nar1c   | AZ23   | 84-155       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 4     | nar1d   | AZ28   | 85-101       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 5     | nar1e   | AZ29   | 77-5-2       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 6     | nar1f   | AZ30   | 85-102       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 7     | nar1g   | AZ31   | 85-103       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 8     | nar1h   | AZ32   | 85-104       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 9     | nar1i   | AZ33   | 85-105       | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 10    | nar1j   | Xno29  | G            | NADH NR structural gene   |
|       |         |        | 84-1020-1034 |                           |
+-------+---------+--------+--------------+---------------------------+
| 11    | nar1k   | EMS29  | 86-117 (1-4) | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 12    | nar1l   | EMS31  | 86-128-6     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 13    | nar1m   | AZ56   | 87-160-4     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 14    | nar1n   | AZ57   | 87-161-14    | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 15    | nar1p   | AZ63   | 87-162-11    | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 16    | nar1q   | AZ64   | 87-163-4     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 17    | nar1r   | AZ65   | 89-144-5     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 18    | nar1t   | AZ67   | 88-324-3     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 19    | nar1ab  | AZ76   | 87-165-3     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 20    | nar1ac  | AZ77   | 87-166-6     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 21    | nar1ai  | AZ79   | 87-167-3     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 22    | nar1aj  | AZ80   | 87-168-7     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 23    | nar1ao  | BSMV1  | 88-272-1     | NADH NR structural gene   |
+-------+---------+--------+--------------+---------------------------+
| 24    | nar2a   | AZ34   | 85-106       | molybdenum cofactor       |
+-------+---------+--------+--------------+---------------------------+
| 25    | nar2ad  | R9401  | 91-393-2     | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 26    | nar2ag  | R9201  | 92-195-2     | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 27    | nar3a   | Xno18  | G84-996-1004 | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 28    | nar3b   | Xno19  | G84-10       | molybdenum cofactor       |
|       |         |        | 05-1019(het) |                           |
+-------+---------+--------+--------------+---------------------------+
| 29    | nar3x   | AZ71   | 92-29-2      | molybdenum cofactor       |
+-------+---------+--------+--------------+---------------------------+
| 30    | nar4y   | AZ72   | 90-43-6      | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 31    | nar5o   | AZ62   | 88-59-5      | molybdenum cofactor       |
+-------+---------+--------+--------------+---------------------------+
| 32    | nar5s   | AZ66   | 93-161-2     | molybdenum cofactor       |
+-------+---------+--------+--------------+---------------------------+
| 33    | nar5u   | AZ68   | 91-378-4     | molybdenum cofactor       |
+-------+---------+--------+--------------+---------------------------+
| 34    | nar6v   | AZ69   | 90-59-8      | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 35    | nar7w   | AZ70   | 86-230       | NAD(P)H NR structural     |
|       |         |        |              | gene                      |
+-------+---------+--------+--------------+---------------------------+
| 36    | nar8z   | AZ73   | 90-86-10     | molybdenum cofactor       |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 37    | nar9ap  | AZ94   | 90-113-6     | nitrate toxicity          |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+
| 38    | \-      | AZ86   | 90-92-2      | nitrate toxicity          |
|       |         |        | (het)        |                           |
+-------+---------+--------+--------------+---------------------------+

**Coordinator's report: Trisomic and aneuploid stocks**

**A. Hang**

USDA-ARS, National Small Grains

Germplasm Research Facility,

Aberdeen, Idaho 83210, USA

There is no new information about trisomic and aneuploid stocks. A list
on these stocks are available in BGN 25:104. Seed request for these
stocks should be sent to the coordinator.

**Coordinator's report: Autotetraploids**

**Wolfgang Friedt**

Institute of Crop Science and Plant Breeding I.

Justus-Liebig-University, Heinrich-Buff-Ring 26-32

## DE-35392 Giessen, Germany

e-mail: wolfgang.friedt@agrar.uni-giessen.de

## Fax: +49(0)641-9937429

The collection of barley autotetraploids (exclusively spring types)
described in former issues of BGN is maintained at the Giessen Field
Experiment Station of our institute. The set of stocks, i.e.
autotetraploids (4n) and corresponding diploid (2n) progenitors (if
available) have last been grown in the field for seed multiplication in
summer 2000. Limited seed samples of the stocks are available for
distribution.

**Coordinator's report: The Genetic Male**

**Sterile Barley Collection**

**M.C. Therrien**

Agriculture and Agri-Food Canada

## Brandon Research Centre

Box 1000A, RR#3, Brandon, MB

Canada R7A 5Y3

E-mail: [MTherrien@agr.gc.ca]{.underline}

The GMSBC has been at Brandon since 1992. If there are any new sources
of male-sterile genes that you are aware of, please advice me, as this
would be a good time to add any new source to the collection. For a list
of the entries in the collection, simply [E-mail]{.underline} me at the
above adress. I can send the file (14Mb) in Excel format. We continue to
store the collection at -20^o^C and will have small (5 g) samples
available for the asking. Since I have not received any reports or
requests the last years, there is absolutely no summary in my report.

**Coordinator's report: Translocations and balanced tertiary trisomics**

## Andreas Houben

## Institute of Plant Genetics and Crop Plant Research

06466 Gatersleben, Germany

email: <houben@ipk-gatersleben.de>

Different translocation lines have been used to evaluate the influence
of recombinantly-elongated chromosome arms on nuclear divisions in
barley. Hudokova *et al*., 2002 confirmed a rule according to which half
the length of the average spindle axis defines the upper tolerance limit
for chromosome arm length. A slightly longer chromosome arm caused
incomplete separation of sister chromatids in similar to70% of mitotic
telophase cells and \>2.5% of daughter cells showing a micronucleus, due
to disruption of non-separated sister chromatids by the newly forming
cell wall. In homozygous condition, this elongated chromosome mediated a
slower growth and reduced fertility of the carrier plants. Its meiotic
transmission was not impaired because of the larger spindle dimensions
in meiocytes as compared to those in mitotic cells.

PCR with the DNA of translocation chromosomes and marker-specific
primers has been used to merge genetically mapped microsatellite (MS)
markers into the physically integrated restriction fragment length
polymorphism (RFLP) map of barley chromosome 3H. It was shown that the
pronounced clustering of MS markers around the centromeric region within
the genetic map of this chromosome results from suppressed
recombination. This yielded a refinement of the physically integrated
RFLP map of chromosome 3H by subdivision of translocation breakpoints
(TBs) that were previously not separated by markers. The physical
distribution of MS markers within most of the subchromosomal regions
corresponded well with that of the RFLP markers, indicating that both
types of markers are similarly valuable for a wide range of applications
in barley genetics (Künzel and Waugh, 2002).

Ten barley translocation stocks have been sent to An Hang (USDA,
Aberdeen, USA). There were no requests for samples of balanced tertiary
trisomics stock collection.

The collection is being maintained in cold storage. To the best
knowledge of the coordinator, there are no new publications dealing with
balanced tertiary trisomics in barley. Limited seed samples are
available any time, and requests can be made to the coordinator.

**References:**

Hudakova S, Kunzel G, Endo TR, Schubert I, 2002: Barley chromosome arms
longer than half of the spindle axis interfere with nuclear divisions.
Cytogenetic and Genome Research 98: 101-107.

Künzel G, Waugh R, 2002: Integration of microsatellite markers into the
translocation-based physical RFLP map of barley chromosome 3H.
Theor.Appl.Genet. 105:660-66

**Coordinator's report: Eceriferum Genes**

**Udda Lundqvist**

SvalöfWeibul AB

### SE-268 81 Svalöv, Sweden

e-mail: [udda@ngb.se]{.underline}

No research work on gene localization has been reported on the
collections of *Eceriferum* and *Glossy* genes since the latest reports
in Barley Genetics Newsletter (BGN). All information and descriptions
done in Barley Genetics Newsletter (BGN) Volume 26 are valid and still
up-to-date. The databases of the Swedish collection has been updated
during the last months and will soon be searchable within International
European databases. As my possibilities in searching literature are very
limited, I apologize if I am missing any important papers. Please send
me notes of publications and reports to include in next year's reports.
Descriptions, images and graphic chromosome maps displays of the
*Eceriferum* and *Glossy* genes are available in the AceDB database for
Barley Genes and Barley Genetic Stocks, and they get currently updated.
Its address is found by: [www.untamo.net/bgs]{.underline}

Every research of interest in the field of *Eceriferum* genes, 'Glossy
sheath' and 'Glossy leaf' genes can be reported to the coordinator as
well. Seed requests regarding the Swedish mutants can be forwarded to
the Nordic Gene Bank, <nordgen@ngb.se>, all others to the Small Grain
Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
[anhang@uidaho.edu](mailto:anhang@uida.edu) or to the coordinator at any
time.

**Coordinator´s report: Nuclear genes affecting the chloroplast**

**Diter von Wettstein**

Department of Crop and Soil Sciences,

Washington State University

Pullman WA 99164-6420, USA

E-mail: <diter@wsu.edu>

The stock list and genetic information presented in the Barley Genetics
Newsletter 21: 102-108 is valid and up-to-date. The stocks have been
transferred to the Nordic Gene Bank. Requests for stocks available for
distribution are to be either sent to:

Dr. Mats Hansson

Department of Biochemistry

Center for Chemistry and Chemical Engineering

Lund University

P.O.Box 124

SE-221 00 Lund, SWEDEN

Phone: +46-46-222 0105

Fax: +46-46-222 4534

E-mail: <Mats.Hansson@biokem.lu.se>

or to

Nordic Gene Bank

P.O. Box 41

SE-230 53 Alnarp

Sweden

Phone: +46 40 536640

FAX: +46 40 536650

e-mail: <nordgen@ngb.se>

**References:**

> A. Hansson, R.D. Willows, T.H. Roberts and M. Hansson 2002. Three
> semidominant barley mutants with single amino acid substitutions in
> the smallest magnesium chelatase sununit form defective AAA^+^
> hexamers. Proc. Natl. Acad. Sci. USA 99: 13944-13949.
>
> U. Olsson, N. Sirijovski and M. Hansson 2003. Characterization of
> eight barley *xantha-f* mutants, deficient in magnesium chelatase.
> *In*: U. Olsson: Ferrochelatase and Magnesium chelatase: Metal
> chelation studies with mutants. pp.146. Doctoral Dissertation,
> Department of Biochemistry, Lund University. (4: 1-13). ISBN
> 91-7422-031-4. E-mail: Mats.Hansson@biokem.lu.se

**Coordinator's report: Semidwarf genes**

**J.D. Franckowiak**

Department of Plant Sciences

North Dakota State University

Fargo, ND 58105, U.S.A.

Zhang 2003 traced the pedigree history of more than 350 dwarf and
semidwarf barley cultivars released in China since 1950. The results
showed that 68.4% of the cultivars were derived from six semidwarf
accessions. 'Chibadamai', 'Xiaoshanlixiahuang', and 'Changzhouluodamai'
are landraces and are in the pedigrees of many semidwarf cultivars
released between 1950 and 1980. They have the same temperature sensitive
dwarfing gene, *uzu* (uzu dwarf), which is located in chromosome 3HL
(Zhang 2000). The other three sources are in released cultivars since
1980. 'Aiganqi' contains the *uzu* dwarfing gene. The dwarfing genes in
'Zhepi 1\' and 'Yanfu Aizao 3\' were not identified. Zhepi 3 was
selected from a cross to Zhaori 19 and released by the Zhejiang Academy
of Agricultural Sciences in 1978. Yanfu Aizao 3, which was released in
Jiangsu Province in 1980, is a gamma-ray induced mutant of the Japanese
barley 'Zaoshu 3\'.

In another paper, Zhang and Zhang 2003 reported their results from
inheritance and allelism tests of reduced plant height using 25 Chinese
accessions. Twenty of the accessions showed monogenic recessive
inheritance patterns and four had digenic recessive patterns. Eleven of
the monogenic accessions and two of the digenic accessions contained
alleles at the *uzu* locus. Accession '1974E' had the *uzu* gene plus a
dominant dwarfing gene. Two of monogenic accessions and one digenic
accession had alleles to the mutant in India Dwarf. Based on the crosses
the *uzu* stock and India Dwarf, eight potential new dwarfing genes were
identified.

Zhang and Zhang 2003 demonstrated that the dwarfing gene in India Dwarf
is not *sdw1* (semidwarf 1). Studies on the dwarfing gene in India Dwarf
are not complete. Thus, its relationship to the *sld5.e* (slender dwarf
5) gene, which was reported to have been derived from Indian Dwarf (CIho
13994) (Franckowiak 2002), is unknown. Because the *sld5.e* gene
backcrossed into Bowman produces relatively weak plants that have little
agronomic potential, different genes may be present in the dwarf
accessions from India. Zhang (personal communications) reported that the
dwarfing gene in Zhepi 1 is allelic to the one present in the Chinese
India Dwarf accession.

The dwarfing gene in Zhepi 1 may be of agronomic interest because it
does not seem to delay maturity like the *sdw1* gene. The *sdw1* gene
delays maturity about three days in the Upper Midwest of the USA and has
not been incorporated into cultivars recommended for malting and brewing
(Hellewell *et al*., 2000). The alleles *sdw1.a* (Jotun) and *sdw1.c*
(denso) at the *sdw1* locus are used to reduce plant height in many
semidwarf cultivars in North America and Europe, respectively (Hellewell
*et al*., 2000).

**References:**

Franckowiak, J.D. 2002. BGS 144, slender dwarf 5, *sld5*. BGN 32:94.

Hellewell, K.B., D.C. Rasmusson, and M. Gallo-Meagher. 2000. Enhancing
yield in semidwarf barley. Crop Sci. 40:352-358.

Zhang, J. 2000. Inheritance of agronomic traits from the Chinese
dwarfing gene donors 'Xiaoshan Lixiahuang' and 'Cangzhou Luodamai'.
Plant Breed. 119:523-524.

Zhang, J. 2003. Inheritance of plant height and allelism tests of the
dwarfing genes in Chinese barley. Plant Breed. 122:112-115.

Zhang, J., and W. Zhang. 2003. Tracing sources of dwarfing genes in
barley breeding in China. Euphytica 131:285-293.

**Coordinator's report: Ear morphology genes**

**Udda Lundqvist**

SvalöfWeibull AB

SE-268 81 Svalöv, Sweden

e-mail: [udda@ngb.se]{.underline}

No new research on gene localization or descriptions on different
morphological genes have been reported since the latest reports in
Barley Genetics Newsletter (BGN). All descriptions made in the volumes
26, 28, 29 and 32 are still up-to-date and valid. The databases of the
Swedish Ear morphology genes have been updated during the last months
and will soon be searchable within International European databases. As
my possibilities in searching literature are very limited, I apologize
if I am missing any important papers. Please send me notes of
publications or reports to include in next year's reports. Descriptions,
images and graphic chromosome maps displays of the Ear morphology genes
are also available in the AceDB database for Barley Genes and Barley
Genetic Stocks, and they get currently updated. Its address is found by
: [www.untamo.net/bgs]{.underline}

Every research of interest in the field of Ear morphology genes can be
report32 are still up-to-date and valid. The databases of the Swedish
Ear morphology genes have been updated during the last months and will
soon be searchable within International European databases. As my
possibilities in searching literature are very limited, I apologize if I
am missing any important papers. Please send me notes of publications or
reports to include in next year's reports. Descriptions, images and
graphic chromosome maps displays of the Ear morphology genes are also
available in the AceDB database for Barley Genes and Barley Genetic
Stocks, and they get currently updated. Its address is found by :
[www.untamo.net/bgs]{.underline}

Every research of interest in the field of Ear morphology genes can be
reported to the coordinator as well. Seed requests regarding the Swedish
mutants can be forwarded to the Nordic Gene Bank, <nordgen@ngb.se>, all
others to the Small Grain Germplasm Research Facility (USDA-ARS),
Aberdeen, ID 83210, USA, [anhang@uidaho.edu](mailto:abhang@uida.edu) or
to the coordinator at any time.

**Coordinator's report: Early maturity genes**

**Udda Lundqvist**

SvalöfWeibull AB

SE-268 81 Svalöv, Sweden

e:mail: [udda@ngb.se]{.underline}

No new research work on gene localization has been reported on the Early
maturity or Praematurum genes since the latest reports in Barley Genetic
Newsletter (BGN). All information and descriptions made in Barley
Genetics Newsletter (BGN) Volumes 26 and 32 are valid and up-to-date.
The database of the Swedish Praematurum genes has been updated during
the last months and will soon be searchable within International
European databases. As my possibilities in searching literature are very
limited, I apologize if I am missing any important papers. Please send
me notes of publications or reports to include in next year's reports.
Descriptions, images and graphic chromosome maps displays of the Early
maturity or Praematurum genes are available in the AceDB database for
Barley Genes and Barley Genetic Stocks, and they get currently updated.
Its address is found by: [www.untamo.net/bgs](http://www.untamo.net/bgs)

Every research of interest in the field of Early maturity or Preamaturum
genes can be reported to the coordinator as well. Seed requests
regarding the Swedish mutants can be forwarded to the Nordic Gene Bank,
<nordgen@ngb.se>, all others to the Small Grain Germplasm Research
Facility (USDA-ARS), Aberdeen, ID 83210, USA,
[anhang@uidaho.edu](mailto:anhang@uida.edu) or to the coordinator at any
time.

**Coordinator's report:Wheat-barley genetic stocks**

**A.K.M.R. Islam**

Faculty of Agriculture & Wine

The University of Adelaide, Waite Campus,

Glen Osmond, S.A. 5064, Australia

The production of amphiploid of *Hordeum marinum* with both durum and
common wheat has been reported earlier. The amphiploid with common wheat
has been backcrossed onto the wheat parent to change the cytoplasmic
background to wheat. It has recently been possible to select six
different monosomic addition lines (1Hm, 2Hm, 4Hm, 5Hm, 7Hm and possibly
6Hm) from among the second backcross progeny (Islam and Colmer,
unpublished).

**Disease and pest resistance genes**

**Brian Steffenson**

Department of Plant Pathology

University of Minnesota

495 Borlaug Hall

1991 Upper Buford Circle

St. Paul, MN 55108-6030, USA

**(in preparation)**