[Answers]{.underline}

[Testing for DON (Vomitoxin)]{.underline}

**A**. **[General Questions]{.underline}**

1\. (a) True

2\. (b) vomitoxin

3\. (a) Official criteria

4\. (b) False

5\. (b) False

6\. (a) True

7\. (b) Dishwashing liquid/water

8.  \(a\) True

9.  \(a\) True

10. \(b\) False

11. \(b\) One

12. \(d\) The file sample

13. \(b\) 200

14. \(c\) Approximately 100

15. \(d\) \"DON equal to or less than 0.5 ppm.\"

16. \(a\) True

17. \(c\) 1000

**B.** **[Neogen AgriScreen Specific Questions]{.underline}**

1.  \(c\) 50 grams of ground sample be placed in a whirlpak with 250 ml
    of

> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3
>
> minutes.

2.  \(b\) False

3.  \(d\) 120 seconds

4.  \(b\) One

5.  \(a\) True

6.  \(a\) One

7.  \(d\) 10 minutes

8.  \(d\) 5

9.  \(b\) False

10. \(a\) True

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C.  **[Neogen Veratox Specific Questions]{.underline}**

1\. (c) 50 grams of ground sample be placed in a whirlpak with 250 ml of

> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3
>
> minutes.

2.  \(b\) False

3.  \(d\) 120 seconds

4.  \(b\) One

5.  \(a\) True

6.  \(a\) One

7.  \(d\) 10 minutes

8.  \(d\) 5

9.  \(b\) False

10. \(a\) True

11. \(d\) Five

**D. [Romer DON Fluoroquant Specific Questions]{.underline}**

1.  \(b\) 50 grams of ground sample be mixed with 200 ml of
    acetonitrile/water and blended

> on high speed for three minutes.

2.  \(b\) False

3.  \(c\) 84% acetonitrile to 16% distilled/deionized water

4.  \(a\) True

5.  \(d\) 70°C

6.  \(b\) False

7.  \(d\) No warm-up is necessary

8.  \(a\) 2 second

9.  \(b\) 10

10. \(a\) True

**E. [Romer AccuTox Specific Questions]{.underline}**

1.  \(c\) 50 grams of ground sample be placed into a plastic or glass
    container with 250 ml of

distilled/deionized water and mixed by shaking (by hand or mechanically)
for 3

> minutes.

2.  \(a\) True

3.  \(c\) 15

4.  \(c\) Four

5.  \(b\) False

6.  \(b\) Yellow

7.  \(d\) Spectrophotometer

8.  \(a\) True

9.  \(b\) False

10. \(a\) 450

**F. [Neogen Veratox 5/5 DON Specific Questions]{.underline}**

1\. (c) 50 grams of ground sample be placed in a whirlpak with 250 ml of

> distilled/deionized water and mixed by shaking (by hand or
> mechanically) for 3
>
> minutes.

2\. (b) False

3\. (d) 120 seconds

4\. (b) One

5\. (a) True

6\. (d) Five

7\. (c) 5 minutes

8\. (c) 5

9\. (a) True

10\. (b) False

11\. d) Equal to or less than 0.5 ppm

**G. [Diagnostix EZ-Quant DON Plate Test - Specific
Questions]{.underline}**

1\. (b) False

2\. (c) 250 ml

3\. (a) True

4\. (d) Nineteen

5.  \(c\) Four

6.  \(b\) Three

7.  \(a\) Two - Three

8.  \(a\) EL-301 microwell reader

9.  \(a\) True

10. \(b\) Ten

11. \(a\) True

12. \(c\) Five minutes

**H. [r-Biopharm Ridascreen Fast DON Test - Specific
Questions]{.underline}**

1\. (c) 250 ml

2\. (a) Two - Three

3\. (d) Distilled or deionized

4\. (d) Four weeks

5\. (d) Nineteen

6\. (b) Three

7\. (d) Three minutes ([+]{.underline} 0.5 minutes)

8\. (b) Ten

9\. (a) EL-301 Microwell reader

10\. (a) True

11. \(a\) 36°F and 46°F

12. \(a\) Two

**I. [Strategic Diagnostics Myco DON Test - Specific
Questions]{.underline}**

1\. (c) 250 ml

2\. (a) Two - Three

3\. (c) Five minutes

4\. (c) Four

5\. (b) Hyperion Microreader 3

6\. (a) True

7\. (a) 36°F and 46°F

8\. (a) Two

9\. (a) Five

10\. (c) Twenty

11. \(c\) 3

12. \(b\) 475

**J. [Vicam DON FQ]{.underline} [Specific Questions]{.underline}**

1\. (b) 50 grams of ground sample be mixed with 200 ml of
acetonitrile/water and blended

> on high speed for three minutes.

2\. (b) False

3\. (c) 84% acetonitrile to 16% distilled/deionized water

4\. (a) True

5\. (d) 70°C

6\. (b) False

7\. (d) No warm-up is necessary

8\. (a) 2 second

9\. (b) 10

10\. (a) True

K.  **[Rosa DON P/N Test - Answers]{.underline}**

1\. (a) True

2\. (a) True

3\. (c) distilled or deionized

4\. (a) 50 grams

5\. (a) 250

6\. (b) 50 µl

7\. (c) 100 µl

8\. (b) False

9\. (a) True

10\. (a) True

11\. (a) True

12\. (a) True

**L. [Diagnostix EZ-TOX Test - Specific Questions]{.underline}**

1\. (b) False

2\. (c) 250 ml

3\. (a) True

4\. (d) twenty

5\. (c) 100

6\. (b) three

7\. (c) one -- two

8\. (a) True

9\. (a) True

10\. (b) 25

11\. (a) True

12\. (a) True

M.  **[Diachemix DON FPA Test - Specific Questions]{.underline}**

1\. (a) two

2\. (d) 500 ml

3\. (b) Sentry® 100 Sample Reader

4\. (b) False

5\. (a) 100 µl

6\. (b) 35

7\. (d) 100 µl

8\. (b) 2

9\. (a) True

10\. (c) 1.0 ppm (≤ 1.0 ppm).

11\. (a) True

12\. (a) True