National Institute on Aging

Aged Rodent Colonies

The National Institute on Aging (NIA), part of the National Institutes
of Health (NIH), maintains colonies of barrier-raised rodents under
contractual arrangement with commercial vendors. All colonies are
barrier maintained and Specific Pathogen-Free (SPF). Animals from the
NIA aged rodent colonies are available to investigators at academic and
non-profit research institutions. The NIA aged rodent colonies are a
limited resource developed for use in aging research. They are not
available for use as a general source of adult animals for unrelated
areas of research.

[Please Note:]{.underline} Beginning in late 2005 or early 2006, rodents
will be provided under contracts with three different contractors --
Harlan Sprague Dawley, Inc. (Harlan), Taconic Farms, Inc (Taconic), and
Charles River Laboratories (CRL). Information on the source of specific
strains and ages is provided in section F and up-dates will be posted on
our website as more information becomes available.

**[A. Contact Information]{.underline}**

Biological Resources Branch (BRB)

National Institute on Aging

7201 Wisconsin Ave. / GW 2C231

Bethesda, MD 20892

Program Assistant in charge of the order desk: Heidi Brogdon

Phone: 301-496-0181

FAX: 301-402-5997

Nancy L. Nadon, Ph.D., Head, Biological Resources Branch

Phone: 301-496-0181

FAX: 301-402-5997

All email correspondence regarding rodent resource orders should be sent
to <rodents@nia.nih.gov>.

This handbook is also available on the web --

<http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/>.

Prices Effective July 1, 2005

**[B. Available Strains]{.underline}**

Inbred Rats: Fischer 344 (F344)

Brown Norway (BN)

Hybrid Rats: F344xBN F1 (F344BN)

Inbred Mice: BALB/cBy

CBA

C57BL/6

DBA/2

Hybrid Mice: CB6F1 (BALB/cBy x C57BL/6)

B6C3F1 (C57BL/6 x C3H)

B6D2F1 (C57BL/6 x DBA/2)

Four-way cross mice (CB6F1 x C3D2F1 (C3H x DBA/2))

Caloric Restricted Rats: F344

BN

F344BN

Caloric Restricted Mice: C57BL/6

B6D2F1

(Entry of mice is expected to begin in late 2005 so old CR mice will not
be available until 2007)

We strive to have animals available out to at least the 50% survival
age, but availability fluctuates due to changes in demand. An idea of
the oldest available for each strain is available at
[[http://www.nia.nih.gov/ResearchInformation/ScientificResources/OldestRodentColonies.htm]{.underline}](http://www.nia.nih.gov/ResearchInformation/ScientificResources/OldestRodentColonies.htm),
but the ages listed on this website are up-dated monthly and are
intended only as an approximation. Contact the order desk for
availability of specific strain/gender/ages.

**[C. Ordering Instructions]{.underline}**

-   All orders for NIA rodents are placed through the **NIA ONLY**.
    Orders must be submitted by FAX (301-402­-5997) or e-mail
    (<rodents@nia.nih.gov> ) ([email is preferred]{.underline}), and all
    inquiries about the status of orders must be directed to the NIA.

-   Payment can be by Purchase Order, an institutional blanket Purchase
    Order to the contractor, Mastercard or VISA. Invoices are sent after
    the animals are shipped -- there is no pre-payment of orders.

-   The purchase order (P.O.) should be made out to the contract
    facility. Invoices are sent by the contractor providing the animals
    and payment is to be made to the contractor's standard billing
    address:

```{=html}
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-   Harlan Sprague Dawley, 3565 Paysphere Circle, Chicago IL 60674

```{=html}
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-   Taconic Farms, Inc., Accounting Department, 273 Hover Ave.,
    Germantown NY 12526

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-   Charles River Laboratories, Inc., GPO Box 27812, New York, NY
    10087-7812

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```
-   By accepting delivery of the animals, you are accepting these terms.
    NIA can refuse to take orders from customers with outstanding
    invoices. Payment is in United States Dollars only.

-   Complete orders received by NIA no later than **Noon Tuesday ET**
    can usually expect next week delivery, dependent on availability.
    Orders must be submitted on the NIA Order Form by email (preferred)
    or FAX. All information requested on the order form must be
    provided. Include the NIH grant number if applicable and make
    special note if the order is part of a pilot study or dissertation
    research support award. Orders may be submitted for future shipping,
    but animals will only be reserved if the order is complete,
    including P.O. \# and desired delivery date(s) or age(s). Type or
    print clearly -- illegible orders will be returned.

-   Animals are sold by age, not weight, and ages are stated in 1 month
    intervals only; all animals born within a calendar month are
    considered to be the same age, so date of birth (DOB) is given as
    month/year. Unless retired breeders are expressly stated when
    ordering F344 males, virgin animals will be shipped.

-   Acknowledgements of orders will be faxed or emailed to the contact
    person for review. If you do not receive an acknowledgement within 2
    working days, contact the order desk as your order may not have been
    received. Contact our office immediately if you notice any
    discrepancies between the order you place and the order
    acknowledgement. **The investigator/purchaser will be responsible
    for incorrectly placed orders that are not corrected through this
    process.** We will email or phone the contact person if there is any
    difficulty filling the order.

-   Changes to or cancellations of orders must be made no later than the
    Thursday before the delivery date, 10 AM ET.

-   Veterans Affairs customers that bill to a VA must be approved every
    fiscal year by the Office of the Veterans Affairs, Chief, Veterinary
    Medical Branch, before animals can be purchased. These approvals
    must be renewed each year.

-   Orders for mid‑December/early‑January delivery should be placed
    early in December. The BRB is closed from Christmas Eve through New
    Year\'s Day. No deliveries will be scheduled the weeks of Christmas
    and New Year's Day.

-   Please note that some of the barriers housing NIA colonies are
    positive for Helicobacter. If your facility requires animals be from
    a Helicobacter-free barrier, you must make that request when you
    submit your order.

-   Note that since the fall of 2003, DBA/2 males at Harlan are provided
    with Shepherd Shacks® in their cages, which has significantly
    reduced aggression against cage mates.

***NOTICE:* If there are any problems regarding the status of animals
upon arrival, you must contact the NIA BRB office within 48 hours of
their arrival.**

**[D. Price Schedule -- Aging Colonies]{.underline}**

The following price schedule applies to animals from the standard aging
colonies, which are ad lib fed and group housed. Pricing for the caloric
restricted colony (caloric restricted and ad lib controls) follows in
section E. Retired Breeders (RB) are only available for F344 Males, 9
months of age and older.

# Age Mice Rats RB (F344 Males only)

1 mo. 12.00 21.00 N/A

2 mo. 15.00 26.00 N/A

3 mo. 18.00 31.00 N/A

4 mo. 21.00 36.00 N/A

5 mo. 24.00 41.00 N/A

6 mo. 27.00 46.00 N/A

7 mo. 30.00 51.00 N/A

8 mo. 33.00 56.00 N/A

9 mo. 36.00 61.00 21.00

10 mo. 39.00 66.00 26.00

11 mo. 42.00 71.00 31.00

12 mo. 45.00 76.00 36.00

13 mo. 49.00 82.00 41.00

14 mo. 52.00 87.00 46.00

15 mo. 55.00 92.00 51.00

16 mo. 58.00 97.00 56.00

17 mo. 61.00 102.00 61.00

18 mo. 64.00 107.00 66.00

19 mo. 67.00 112.00 71.00

20 mo. 70.00 117.00 76.00

21 mo. 73.00 122.00 82.00

22 mo. 76.00 127.00 87.00

23 mo. 79.00 132.00 92.00

24 mo. 82.00 137.00 97.00

25 mo. 86.00 143.00 102.00

26 mo. 89.00 148.00 107.00

27 mo. 92.00 153.00 112.00

28 mo. 95.00 158.00 117.00

29 mo. 98.00 163.00 122.00

30 mo. 101.00 168.00 127.00

31 mo. 104.00 173.00 132.00

32 mo. 107.00 178.00 137.00

33 mo. 110.00 183.00 143.00

34 mo. 113.00 188.00 148.00

35 mo. 117.00 193.00 153.00

36 mo. 120.00 198.00 158.00

and older

**[E. Caloric Restricted Colony]{.underline}**

In the caloric restricted colony, rats are \$12.00 per month of age and
mice are \$6.00 per month of age. These prices apply to both
caloric‑restricted (CR) and [ad]{.underline} [libitum]{.underline} (AL)
controls from this colony. Both CR and AL are individually housed. CR is
initiated at 14 weeks of age at 10% restriction, increased to 25%
restriction at 15 weeks, and to 40% restriction at 16 weeks where it is
maintained throughout the life of the animal. CR/AL rats are sold only
by age in months, i.e. 4 months or 24 months, not by weight or age in
weeks. Survival curves, food intake curves and body weights of the NIA
strains have been published (Turturro et al. (1999) Growth curves and
survival characteristics of the animals used in the biomarkers of aging
program. J. Gerontology 54A:B492-B501).

Feeding instructions will be provided with the caloric restricted
animals. If you are ordering food, please indicate how long you will
maintain the animals so that the contractor can calculate how much food
you will need. When food is shipped by truck, there is a flat rate
freight charge of \$13.00 per type of food (AL or CR). Food shipped by
air freight or common carrier is subject to prevailing rates of the
carrier. NIH31/NIA Fortified (for CR feeding) is available in weighed
pellets at 0.5 gram intervals from 2.5 grams to 5.0 grams, at
\$10.00/Kg. NIH31 (for AL feeding) is only available in random weight
chunk formula, at \$2.00/Kg. Formulation of the diets is available from
the BRB.

**[F. Location of colonies]{.underline}**

Beginning in late 2005 or early 2006, the NIA aged rodent colonies will
be transitioning to new contracts, utilizing three different
contractors. For a period time, young and old animals may be shipped
from different contractors, requiring two Purchase Orders for a single
order of animals. Orders for animals from different contractor
facilities must be submitted on separate completely filled out NIA order
forms. The following chart indicates which contractor will house the
different strains. Our website will be up-dated to show the date of
birth (DOB) at which the transition is made from one contractor to
another. Contact the order desk for clarification of location.

+-------------+------------+-------------+-------------+--------------+
|             | Aging      | Aging       | Aging BN    | Caloric      |
|             |            |             | and F344xBN | Restricted   |
|             | Mice       | F344 rats   | F1 rats     |              |
|             |            |             |             | Rats and     |
|             |            |             |             | Mice         |
+-------------+------------+-------------+-------------+--------------+
|             |            |             |             |              |
+-------------+------------+-------------+-------------+--------------+
| Current     | Harlan     | Harlan      | Harlan      | Harlan       |
|             |            |             |             |              |
| Contractor  |            |             |             |              |
+-------------+------------+-------------+-------------+--------------+
| New         | Charles    | Taconic     | Harlan      | Charles      |
|             | River      | Farms       |             | River        |
| Contractor  |            |             |             |              |
|             | La         |             |             | Laboratories |
|             | boratories |             |             |              |
+-------------+------------+-------------+-------------+--------------+
| Transition  | To be      | To be       | Not         | To be        |
|             | determined | determined  | applicable  | determined   |
| Da          |            |             |             |              |
| te-of-Birth |            |             |             |              |
+-------------+------------+-------------+-------------+--------------+

**[G. Shipment of Animals]{.underline}**

The objective for maintaining these colonies is to provide animals for
aging research that are in good health, but characteristic of aging
animals. Therefore, the best looking animals are [not]{.underline}
selected for shipping, but rather, animals are selected at random. This
means, particularly in older animals, that you may get some \"scruffy\"
looking animals or animals exhibiting common conditions of aging such as
small tumors, cataracts, loss of hair, stiffness of limbs, etc. Tumors
in particular are commonplace in older rodents. These conditions are not
justification for replacement. If your protocol requires animals free of
such conditions, it is your responsibility to order enough animals to
allow you to select preferred animals. You may request "No Tumors" or
"No Cataracts" on the order form but that will only apply to visible
tumors or cataracts. It will not guarantee that small tumors or
cataracts will not be present in the animals shipped.

Occasionally, periods of high or low temperature make the air shipment
of aged animals extremely risky. Animals exposed to hot or cold loading
docks and airplane holds under these conditions are quite likely to die
or to suffer temperature stress. When such conditions exist, we will
call you to inform you of the delay of the shipment until the
temperature moderates. The NIA will not ship animals by air during such
weather advisories. Shipments made by environmentally controlled trucks
are not affected by weather except during severe storms. If you have
questions about this policy, please contact the NIA office.

To calculate shipping/transportation costs: You pay for each crate and
to ship each crate. We do not mix ages or animals coming out of
different barriers/areas. We recommend air shipment of caloric
restricted animals for locations where truck delivery would require more
than one day transit time. BALB/cBy males are currently being shipped in
compartmentalized crates, maximum of 5 mice per crate, to prevent
fighting. Shipping costs for each contractor are below. The maximum
number of animals per crate is 6 (Harlan) or 10 (Taconic) rats or 20
mice from the aging colonies, depending on age and size, and 2 rats from
the caloric restricted colony.

[Harlan]{.underline}

U.S. Truck Crate \$11.50 each

International Air Crate \$13.50 each

Harlan Truck Freight \$28.00 per crate (subject to change)

Air Freight-Domestic \$35.00 - \$80.00 per crate (subject to change)

Gel Transit Packets \$1.80 each (2 required per crate)

Water kits \$5.50 each (optional)

[Taconic Farms]{.underline}

Taconic Transit Cage (TTC) \$18.14 each

Truck Freight \$10.76 per TTC (subject to change)

Air Freight-Domestic \$30.00 - \$75.00 per crate (subject to change)

Gel Transit Packets (included in cost of TTC)

Taconic uses poly-screened TTCs for air shipments and non-poly screened
TTCs for truck shipments. Use of poly-screened TTCs for truck shipments
can be requested on the order form.

[Charles River Laboratories]{.underline}

Shipping Crate \$10.20 each

Truck Freight \$20.60 per crate (subject to change)

Air Freight-Domestic \$30.00 - \$75.00 per crate (subject to change)

Gel Transit Packets (included in cost of crate)

**[H. Animal Replacement Policy]{.underline}**

The NIA will provide no-cost replacement or credit for animals that are
dead on arrival or die within 48 hours of arrival. Replacements will be
of the same date of birth as originally shipped. If animals are
determined to be ill or injured at arrival and a replacement or credit
is requested, a veterinary report must be provided. All animals are
maintained in barrier conditions under a health monitoring program and
are assumed to be in good health when leaving the NIA colonies. The
development of tumors is normal in these animals with aging, therefore
the presence of tumors is not an acceptable criteria for replacement.
Likewise, a scruffy or unkempt appearance is often normal for very aged
animals and is not reason for replacement or credit. Since we have no
control over the conditions in the facilities into which these animals
are shipped, we will not accept responsibility for their health after 48
hours of receipt. Therefore if you have any problems regarding the
status of your animals upon their arrival, it is important that you
contact the NIA BRB **within 48 hours of receipt**.

**[I. Optional Services]{.underline}**

NIA rodents are tattooed on the tail with the month and year of birth.
An independent service is available from Harlan to implant Avid
Microchips under the skin, at \$12.50 per animal. Harlan also provides
various surgical procedures at their BioTech Center, as described on
their website
([http://www.harlan.com/contract%20breeding/surgical.html](http://www.harlan.com/contract breeding/surgical.html)).

Taconic performs surgical procedures as an independent service -- for
prices, please go to page 34 of the Taconic Price manual --

<http://www.taconic.com/Product_Guide/2005_USPriceManual.pdf> .

Charles River Laboratories (CRL) implants AVID Microchips as an
independent service, at a total cost of \$21.90 per animal. Surgical
procedures are also provided by CRL as independent services and prices
are on pages 32 and 33 of the CRL Research Models & Services catalog --

[[http://www.criver.com/general/2005_Research_Model_Services_Catalog.pdf]{.underline}](http://www.criver.com/general/2005_Research_Model_Services_Catalog.pdf)
.

**[J. Colony Monitoring and History]{.underline}**

NIA mice and rats are monitored on a regular basis to detect genetic
changes that could result from contamination. Colony animals are also
monitored quarterly for pathology and evidence of pathogens. The results
of the health monitoring for animals in the same room as your shipment
will be sent with every animal shipment. You may request that the health
report be sent before the animals are shipped. **Please note that some
of the barriers housing NIA colonies are positive for Helicobacter. If
your facility requires animals be from a Helicobacter-free barrier, you
must make that request when you submit your order.**

Inbred and hybrid mice in this NIA colony were derived from NIH
pedigreed breeding stock in 1974 and for many years were maintained as
closed inbred colonies. As mutations occur in both the NIA colony and
the NIH breeding colonies, the NIA and NIH genotypes would undoubtedly
drift apart. The solution to this problem is to periodically rederive
NIA substrains from a common pedigreed breeding stock. One disadvantage
to this procedure is that if the rederivation occurs in the middle of an
ongoing research project, some differences in genotype of animals used
early and late in the project may be unavoidable. Whether these
differences pose a research problem or not is largely a matter of
chance, depending upon whether significant mutations have occurred and
which genetic loci are involved.

Since the problems of substrain differentiation are more significant to
NIA programs as a whole, NIA initiated a policy of rederivation from
pedigreed stock every 6-7 years. This rederivation interval should keep
substrain differentiation at a minimum and still minimize potential
disruption of ongoing research. The first rederivation of the NIA mouse
colonies occurred in 1983, with subsequent rederivations in 1990, 1998,
and 2005. The 1990, 1998 and 2005 rederivations used Jackson Laboratory
stock.

The Fischer 344 rat colony was rederived in 1990 using Harlan Sprague
Dawley Inc.'s commercial pedigreed stock that had been rederived from
NIH stock in 1988, and again in 1998 and 2005 using NIH stock. In
September 1985, a colony of Brown Norway (BN) rats and the F1 hybrids of
the F344 X BN cross was established to provide an alternative model to
the Fischer 344, based on studies that showed the F344 x BN cross
produced progeny with fewer detrimental pathologies than inbred strains.
Rederivation of this colony occurred in 1992 and again in 1999, using
BN/BiRij stock originating from TNO, The Netherlands, and F344 stock
from the NIH. The last rederivation was in 2005, using stock from the
NIH for both strains.

The Caloric Restricted colony currently includes three rat genotypes and
two mouse genotypes maintained under conditions similar to our other
colonies except that they are individually caged. Ad libitum-fed
controls maintained under the same conditions as restricted animals are
also available. The rats were originally derived from NIH stock, and the
colony was rederived in 1998 and again in 2005, using NIH stock for both
rat strains. The mice in the caloric restricted colony were rederived in
1998 and in 2005 using stock from The Jackson Laboratory.

**[K. Barrier Environmental Information]{.underline}**

+----------+------------------+-------------------+------------------+
|          | Harlan           | Charles River     | Taconic Farms    |
+----------+------------------+-------------------+------------------+
|          |                  |                   |                  |
+----------+------------------+-------------------+------------------+
| Tem      | 72^o^ -- 77^o^ F | 67 ^o^ -- 73 ^o^  | 68 ^o^ -- 72 ^o^ |
| perature |                  | F                 | F                |
+----------+------------------+-------------------+------------------+
| Humidity | 40-55%           | 35 -- 55%         | 30-70%           |
+----------+------------------+-------------------+------------------+
| Water    | pH 5.8-6.0, 7-8  | pH 7.0-7.5, 4-6   | pH 6-8, 4-10 ppm |
|          | ppm chlorine     | ppm chlorine      | chlorine         |
+----------+------------------+-------------------+------------------+
| Li       | 12 hr./12 hr.    | 12 hr./12 hr.     | 12 hr./12 hr.    |
| ght/Dark |                  |                   |                  |
|          |                  |                   |                  |
| Cycle    |                  |                   |                  |
+----------+------------------+-------------------+------------------+
| Bedding  | Sterilized Aspen | Wood shavings     | Sani-chip wood   |
|          | (BN, F344BN)     |                   | shavings         |
|          | (Harlan Product  |                   |                  |
|          | T.7083) or       |                   |                  |
|          |                  |                   |                  |
|          | Tek-Fresh (mice, |                   |                  |
|          | F344) (Harlan    |                   |                  |
|          | product T.7099)  |                   |                  |
+----------+------------------+-------------------+------------------+
| Feed     | Sterilized NIH31 | Sterilized NIH31  | Sterilized NIH31 |
|          | or               | or                |                  |
|          | NIH31-Fortified  | NIH31-Fortified   |                  |
|          | (CR animals      | (CR animals only) |                  |
|          | only)            |                   |                  |
+----------+------------------+-------------------+------------------+
| Caging   | Aging -- 3 rats  | Aging -- 5 mice   | 5 rats per cage  |
|          | or 5 mice per    | per cage; CR --   |                  |
|          | cage;            | singly housed     |                  |
|          |                  |                   |                  |
|          | CR -- singly     |                   |                  |
|          | housed           |                   |                  |
+----------+------------------+-------------------+------------------+

ORDER FORM - NIA AGED RODENT COLONIES

Orders [MUST]{.underline} be placed by NOON ET **Tuesday** for following
week delivery. We require orders to be placed by fax or email. FAX \# at
Order Desk 301-402-5997, Voice \# 301-496-0181,

email: <rodents@nia.nih.gov>. (emergency back up fax \# 301-402-0010).

Contractor information for specific colonies:
<http://www.nia.nih.gov/ResearchInformation/ScientificResources/AgedRodentColoniesHandbook/LocationofColonies.htm>

Please type or print and provide [all]{.underline} information. We can
only offer following week delivery for those orders for which
[completed]{.underline} order forms are received by the deadline of
Tuesday noon, Eastern time.

  ---------------- ----------- ----------- ----------- ----------- ------------------------------------
  Principal                                            **     **   
  Investigator's                                                   
  Name:                                                            

  Principal                                            **     **   
  Investigator's                                                   
  email:                                                           

  Project title:               **     **                           

  Project funding                                                  **     **
  source and grant                                                 
  number:                                                          

  Institution:     **     **                                       

                                                                   

  Shipping                                 **     **               
  Address:                                                         

                                           **     **               

                                           **     **               
  ---------------- ----------- ----------- ----------- ----------- ------------------------------------

  -------------- --------------------------------------------------------
  Billing        **     **
  Address:       

                 **     **

                 **     **
  -------------- --------------------------------------------------------

  ---------- ----------- ------------- ----------------- -- -------------------------------
  Contact                              **     **            
  Person's                                                  
  Name:                                                     

  FAX:       **     **                                      

  Phone:                 **     **                          

  Email:                 **     **                          
  ---------- ----------- ------------- ----------------- -- -------------------------------

  -------- ------------------------ -- ------------- -------------------- --
  P.O. \#  **     **                   Delivery      **     **            
  :                                    date:                              

  -------- ------------------------ -- ------------- -------------------- --

Orders must be received by **[noon]{.underline} Tuesday (ET)** for
following week delivery.

  ------------------- ---------- ---------- ----------- -----------------------
  Strain              Quantity   Age (mon)  Gender      Additional Information

  **     **           **   **    **  **     **     **   **     **

  **     **           **   **    **  **     **     **   **     **

  **     **           **   **    **  **     **     **   **     **

  **     **           **   **    **  **     **     **   **     **
  ------------------- ---------- ---------- ----------- -----------------------

Special Instructions: **     **

REQUEST FOR NIA ANIMAL CREDIT OR REPLACEMENT

Credits / Replacements will be considered if requested within 48 hours
of receipt of the animals.

# PROVIDE ALL REQUESTED INFORMATION - PLEASE TYPE OR PRINT CLEARLY

  ----------------------------------- -----------------------------------
  **DATE OF REQUEST:**                     

  ----------------------------------- -----------------------------------

  ----------------- -- -------- -- ------------- -------------------- ---------------
  **Requesting:**      Credit      Replacement   Delivery Day              
                                                 Requested:           

  ----------------- -- -------- -- ------------- -------------------- ---------------

  --------------------- -------- ------------ ----------------------------------------
  Principle                                   
  Investigator:                               

  Institution:                                

  Person Requesting                                
  Credit/Replacement:                         
  --------------------- -------- ------------ ----------------------------------------

  --------- ------- ------- ----------- ----------- ----------- ------- --------------- ------------
  Title:                                Email                                           
                                        Address:                                        

  Fax                                   Phone                                           
  Number:                               Number:                                         

  NIA Order                                         Delivery                                 
  Number:                                           Date                                
                                                    Received:                           
  --------- ------- ------- ----------- ----------- ----------- ------- --------------- ------------

Person who unpacked the animals:

  -------- ----------------------- -------- --------------- ------ --------------
  Email:                           Phone:                   Fax:        

  -------- ----------------------- -------- --------------- ------ --------------

Please provide a detailed description of the animals upon arrival, and
the condition of the containers they arrived in:

  -----------------------------------------------------------------------
       

       

       

       

       

       
  -----------------------------------------------------------------------

NOTE: Animals will be replaced at the same age as the original order,
unless the customer specifically indicates the same DOB.

  ------------ ---------------------------- -------------- -------------- --------------
  **Gender**   **Strain**                   **Quantity**   **Age**        **DOB**

                                                                               

                                                                               

                                                                          
  ------------ ---------------------------- -------------- -------------- --------------

Please type in fields and email back as an attachment to
<rodents@nia.nih.gov> . If you choose to fax form back to BRB, please
fax it to 301-402-5997.