**2007**

**ANNUAL OPERATING PLAN**

**for**

**FISH PRODUCTION PROGRAMS**

**in the**

**CLEARWATER RIVER BASIN**

by

U.S Fish and Wildlife Service

Idaho Department of Fish and Game

Nez Perce Tribe Fisheries

March 8, 2007

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(bet you wish more of them were)

**CONTENTS**

**CLEARWATER BASIN ANNUAL OPERATING PLAN (AOP) 2007**

Page

**1. Steelhead** 1

1.  Broodyear 2006 Steelhead 1

    1.  Dworshak 1

    2.  Clearwater 1

    3.  South Fork Un-clipped Releases 2

2.  Broodyear 2007 Steelhead 3

    1.  Dworshak 3

    2.  Kooskia 5

    3.  Clearwater 5

    4.  South Fork Un-clipped Releases 6

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1.  **Spring Chinook Salmon** 6

    1.  Broodyear 2005 Spring Chinook 6

        1.  Dworshak 6

        2.  Kooskia 7

        3.  Clearwater 8

    2.  Broodyear 2006 Spring Chinook 9

        1.  Dworshak 9

        2.  Kooskia 10

        3.  Clearwater 10

        4.  Nez Perce Tribal Hatchery 11

    3.  Broodyear 2007 Spring Chinook 13

        1.  Dworshak 13

        2.  Kooskia 14

        3.  Clearwater 15

        4.  Nez Perce Tribal Hatchery 16

2.  **Coho** 18

    1.  Broodyear 2005 Coho 19

        1.  Dworshak 19

        2.  Transfers (Eagle Creek NFH) 19

    2.  Broodyear 2006 Coho 19

        1.  Dworshak 19

        2.  Clearwater 20

        3.  Transfers (Eagle Creek NFH) 20

    3.  Broodyear 2007 Coho 21

        1.  Dworshak 21

3.  **Fall Chinook Salmon** 21

    1.  Broodyear 2005 Fall Chinook 21

        1.  NPT Fall Chinook Acclimation Project -- Big Canyon Facility
            21

    2.  Broodyear 2006 Fall Chinook 22

        1.  NPT Fall Chinook Acclimation Project -- Big Canyon Facility
            22

        2.  Nez Perce Tribal Hatchery 23

        3.  Dworshak NFH 24

    3.  Broodyear 2007 Fall Chinook 25

        1.  Adult Collection 25

4.  **Rainbow Trout** 27

    1.  USFWS Program 27

        1.  Dworshak Kids' Fishing Day 27

    2.  IDFG Programs 27

        1.  Dworshak Reservoir 28

        2.  Clearwater Basin 28

5.  **Pacific Lamprey** 28

    1.  NPT Program 28

        1.  Nez Perce Tribal Hatchery 28

6.  **Contacts** 29

    **CLEARWATER BASIN ANNUAL OPERATING PLAN (AOP) 2007**

**Version 3/8/2007 11:31 PM**

(Each section names a contact person for additional information,
coordination, or notification -- contact information is listed in
Section 7.)

1.  **STEELHEAD**

> *The total adult return goal for Dworshak NFH and Clearwater Hatchery
> is 34,000 steelhead to the project area. Broodstock for all facilities
> are collected at Dworshak and total 4,300 adults. Additional details
> are listed in the pertinent sections below.*

1.  **Broodyear 2006 Steelhead**

    1.  **Dworshak** -- *Broodstock need for Dworshak mitigation is
        \~2,300 fish, this number of steelhead is needed to provide
        enough males to allow a 1:1 spawning ratio for the 630 females
        needed for egg collection. (An additional 2,000 fish are needed
        to provide eggs for Clearwater and Magic Valley Hatchery
        steelhead programs.) Male to female return ratio for two ocean
        steelhead at Dworshak is typically 1:3, so to collect enough
        males, more females than needed are collected and then excess
        steelhead are typically outplanted for natural spawning. This
        number includes jacks, accounts for pre-spawning mortality, and
        the 500 steelhead that are collected in the fall to cover the
        early returning, early spawning component of the run. This brood
        level provides \~2.1 million smolts at an average of 80% eyed
        egg-to-smolt survival to meet the adult return goal of 20,000 to
        the Clearwater River.*

        1.  [Production status]{.underline} - As of December 1, 2006
            there were a total of 2.17 million steelhead on station, 153
            mm average total length, 13 fpp. Length frequency and pound
            counts are performed monthly on representative ponds.
            *Thomas Trock*

        2.  [Projected release]{.underline} -- Offsite release is
            expected to occur the week of April 9, 2007. Onsite release
            is expected to occur the week of April 16. DNFH expects to
            release a total of 2.0+ million steelhead, including
            \~220,000 un-clipped, at an average total length \> 200 mm
            (6 fpp). *Thomas Trock*

        3.  [Fish health status]{.underline} - June started the
            IHNV/Coldwater Disease event. This outbreak started early,
            and has caused chronic mortality as seen in previous years.
            The reuse system was started in December, allowing the fish
            to gain a larger size and ability to resist *Ich* infections
            better. The combination of the two pathogens weakened the
            fish enough in past years that many were not able to
            withstand the infection. As size continues to increase, fish
            immune systems should be better able to fight the
            infections. A 60 fish sample will be tested for viral,
            bacterial, and parasitic pathogens prior to release. *Kathy
            Clemens*

        4.  [M&E]{.underline} -- Six CWT groups for system contribution
            and early return groups, and 1,500 PIT tags for Fish Passage
            Center emigration timing \[ex. BY05 = 66.7%, 14.3 days\].
            Prior to release 500 marked fish from each mark group (tag
            code) are checked for tag retention (ex. BY05 = 98.7%). Also
            500 fish are checked for LV clip and AD clip quality. Each
            pond with un-clipped fish is sampled (100 fish) for dorsal
            fin erosion and presence of AD fins. *Jill Olson / Ralph
            Roseberg*

    2.  **Clearwater** - *Original design memorandum shows the
        production goal may be as high as two million steelhead smolts.
        Historically, the steelhead smolt releases from Clearwater Fish
        Hatchery have ranged from approximately 600 to 1.04 million.
        Adult return goal for the program is 14,000 steelhead.*

        1.  [Production status / projected release]{.underline} - The
            estimated number of BY06 steelhead to be released in the
            spring of 2007 is 868,500. 272,500 AD-clip production into
            the lower SF Clearwater, 345,000 no ad-clip and 251,000
            ad-clip production will be released into the upper SF
            Clearwater River pursuant to the US v. Oregon 2005-2007
            Interim Management Agreement. (**Table 1**) *Jerry McGehee*

        2.  [Fish health status]{.underline} - For Egg Disease
            Certification, all females are sampled (individually) for
            viral replicating agents. Eggs from any females that test
            positive are destroyed, and only eggs that test negative for
            IHNV are taken to CFH. Juvenile rearing inspections are
            performed quarterly by Eagle Fish Health Lab. No
            prophylactic treatments are used during steelhead rearing.
            Inspections are conducted quarterly. No pathogens detected
            to date on inspection sampling. Diagnostics on demand.
            Pre-liberation samples performed on 20 fish sample prior to
            release. Viral pathogens have not been detected in these
            fish. *Flavobacterium psychrophilum, Aeromonas hydrophila,
            and Aeromonas sobria* were detected in all fish sampled in
            the outdoor raceways after these fish were marked. Mortality
            was not high enough to warrant treatment. *Doug Munson*

        3.  [M&E]{.underline} - The fish are sampled monthly between the
            25th and 28th of the month. During months of rapid growth,
            fish are sampled biweekly. Pound counts are taken to track
            fish growth and monitor if growth is following the annual
            growth projections. Length frequencies are taken monthly.
            Approximately 30 days prior to release, 100 fish are sampled
            to quality check adipose (Ad) fin clips, ventral fin clips,
            and coded wire tag (CWT) retention. *Jerry McGehee*

    3.  **South Fork Un-clipped Releases** - *533,000 un-clipped
        steelhead is the program goal for SF Clearwater releases stated
        in the harvest agreement between the States, Tribes and Federal
        parties. The agreement of releasing un-clipped fish was to
        offset reductions in down-river Tribal fisheries. The principle
        is that the returning un-clipped adult steelhead will escape the
        sport fishery therefore return at higher numbers to tributaries,
        to hopefully spawn, thereby increasing natural production.*

        1.  [Production status]{.underline} -- See Dworshak NFH and
            Clearwater FH for information.

        2.  [Projected release]{.underline} -- 333,000 unclipped
            steelhead from Clearwater Hatchery will be released into the
            South Fork tributaries and Lolo Creek. IDFG and the NPT will
            coordinate the transport of the Lolo Creek release group.
            Approximately 200,000 unclipped steelhead from Dworshak NFH
            will be released into Newsome Creek and American River
            (50:50 split). The COE will transport the steelhead to
            American River and Newsome Creek and the NPT will assist
            with release site logistics. (**Table 1**) *Howard Burge*

        3.  [M&E]{.underline} - Broodyear 03, 04 and 05 steelhead were
            uniquely marked by the FWS-IFRO with PIT, CWT, and elastomer
            tag groups to evaluate acclimated vs. direct release affects
            and upriver versus downriver groups**.** FWS will also be
            analyzing tributary of release effect by comparing Red R
            (upriver) to Mill/Meadow (downriver). IFRO is also
            evaluating if unclipped steelhead perform differently than
            production steelhead by comparing Red River unclipped and
            production releases as paired release. Additional monitoring
            includes determination of any un-clipped steelhead straying
            into and spawning in wild production areas such as the
            Lochsa or Selway basins. 2006 was the last release year of
            uniquely marked fish for this study, however evaluation of
            returning adults will continue through 2009. *Howard Burge*

2.  **Broodyear 2007 Steelhead**

    1.  **Dworshak**

        1.  [Projected adult return]{.underline} - Based on the expanded
            number of 1,090 I-salt rack returns, the predicted steelhead
            return to Dworshak NFH in 2006-2007 should be about 9,385
            which is above average. In-season hatchery "B" steelhead
            estimates at both Bonneville and Lower Granite dams have
            caused the estimate to be revised slightly upward. (**Table
            2b**). *Jill Olson / Ralph Roseberg*

        2.  [Ladder operation]{.underline} - The ladder was open October
            2-6, 2006 and reopened October 11-13 for collection of
            early-return steelhead. During this period there were 515
            early-run steelhead collected (5 "wild" returned to river)
            for a total of 510 held for spawning in the spring of 2007.
            It was reopened two-three days/week until November 27 to
            collect coho. There were 1,941 excess adult steelhead
            trapped which were outplanted to Hog Island. There were also
            fall Chinook trapped along with the steelhead and coho at
            this time. Based on the steelhead returns we are planning on
            intermittent ladder operation to prevent excess fish
            collection. This also keeps steelhead in the river where
            they are available for sport and tribal harvest and allows
            us to spawn fish that have not been held in the hatchery for
            more than a few days. Ladder operation may be modified
            in-season if weekly goals are not met. The ladder will be
            reopened February 26, 2007 for the collection of mid and
            late returning steelhead. Surplus adults will be outplanted
            by the Nez Perce Tribe late in the season. The outplanting
            schedule will be coordinated with Mike Key, NPT. *Thomas
            Trock*

        3.  [Adult fish health]{.underline} -- 70 males were injected
            with the hormone sGnRHa prior to spawning, using the implant
            form, under INAD. Fish are treated three times per week with
            formalin for fungus, under a veterinary prescription. A
            minimum of 60 tissues samples, and 150 ovarian fluid samples
            will be collected at spawning and assayed for viruses,
            bacteria, and parasites. *Kathy Clemens*

        4.  [Adult outplanting/marking]{.underline} -- Ladder opening
            for collection of spring returns is not planned until
            February 23. Any excess steelhead collected will be put in a
            separate holding pond until the NPT outplant them in SF
            Clearwater River tributaries. These fish will all be marked
            with left

> opercle v-notch. Due to the planned intermittent ladder operation in
> 2007 we will need to expand the number collected to estimate what the
> rack return would have been if we had not closed the ladder. Like
> 2003-06 we will use the average conversion between Lower Granite and
> Dworshak NFH to estimate 2 &3-ocean hatchery 'B' steelhead. *Jill
> Olson / Ralph Roseberg*

5.  [Carcass disposition]{.underline} - Adult carcasses is now being
    coordinated through the Regional Office and details are still being
    worked out at this time. If distribution points become limiting
    Howard Burge will coordinate with Tom Rogers. *Thomas Trock*

6.  [Adult M&E]{.underline} -- System contribution, and early return CWT
    are being recovered for all three age classes. Blank wire is being
    recovered for the three ocean age class. FWS-IFRO is radio tagging
    un-clipped dorsal eroded adult steelhead (VIE identified fish will
    be tagged for BY07) at Lower Granite Dam to evaluate returns from
    that supplementation program. Returning adults are measured and
    examined for gender, various clips and tags, and seal bites or other
    injuries, then sorted for spawning or holding. The protocol for
    handling of suspected blank-wire-tagged / un-clipped adipose fin
    steelhead is to identify them as they came across the sorting table.
    Mark them with a cable tie and send them down to the spawning area.
    They will be spawned if we needed them and they are ripe. If we do
    not need them for spawning we will kill them for tags and record if
    they were ripe or not. *Jill Olson / Ralph Roseberg* / *Howard
    Burge*

7.  [Spawning/egg take plans, mating protocol]{.underline} - Current
    plans are to take 2.8 million eyed eggs for Dworshak, \~1.3 million
    green for Clearwater and \~1.5 million for Magic Valley. Potlatch
    will receive approximately 18,000 green eggs. **Table 3**. If
    cryopreserved sperm is needed contact Bill Young (NPT, 208-634-5290)
    for a request form. *Thomas Trock*

8.  [Juveniles Production]{.underline} - [Incubation]{.underline}:
    Dworshak will incubate eggs from approximately 630 steelhead females
    for its program, 150 fall-return adults and 480 from winter and
    spring returns. After eye-up and enumeration, approximately 2.8
    million eyed eggs will go into the Dworshak program. Dworshak will
    also provide incubation space for up to 1.4 million green eggs for
    Clearwater Fish Hatchery. *Thomas Trock*

> [Nursery Rearing]{.underline}: Dworshak will early-rear 2.5 million
> steelhead in its nursery until the fish reach approximately 80 fpp
> during the spring and summer of 2007.
>
> [Outside Rearing]{.underline}: Approximately 2.35 million steelhead
> will be moved from nursery tanks to 82 outside Burrows Ponds from the
> end of May until September, 2007. Fish will be ponded at final rearing
> densities, \~28,500 fish/pond. Most steelhead transferred outside will
> receive an adipose-fin clip when ponded to designate it as a hatchery
> fish. *Thomas Trock*
>
> [SF releases]{.underline} -- Approximately 200,000 of Dworshak NFH
> reared steelhead are programmed for American River and Newsome Creek
> releases as part of the US v. OR supplementation agreement. *Howard
> Burge*

9.  [Juvenile Fish health]{.underline} - Upon ponding, will be monitored
    for coldwater disease and parasites. A 60 fish sample will be tested
    for viral, bacterial, and parasitic pathogens prior to release.
    *Kathy Clemens*

10. [Planned juvenile marking & tagging, release sites]{.underline} -
    Marking plans for BY07 steelhead at Dworshak NFH are found in
    **Table 4**. *Jill Olson / Ralph Roseberg*

11. [Juvenile M&E]{.underline} FWS will be CWT steelhead from the three
    systems and early return progeny. *Howard Burge*

12. [Communication]{.underline} FWS puts out weekly spawning reports and
    weekly return reports, and annual spawning and adult return reports
    are also produced.

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2.  **Kooskia**

    1.  [Weir/trap operation]{.underline} - The adult trap will be
        opened sometime in mid-March. The trap will remain opened until
        late April. *Howard Burge*

    2.  [Adult handling/outplanting/marking]{.underline} - All natural
        (unmarked) fish will be passed upstream of the weir. CWT
        steelhead will be sacrificed for tag recovery. Adult hatchery
        steelhead (not taken for CWT) will be loaded into NPT truck for
        outplanting at time of sorting; NPT contact will be Mike Key.
        Outplanted steelhead will be given a right opercle v-notch. Any
        Tribal requests for steelhead will be coordinated through Nancy
        McAllaster, NPT (208-843-7320 ext.2445). Other native species
        (bull trout, suckers, whitefish etc.) trapped will be passed
        upstream above the weir. *Jill Olson / Ralph Roseberg*

    3.  [M&E]{.underline} - Returning adults are measured and examined
        for gender, various clips and tags, and seal bites or other
        injuries, then sorted for spawning or holding. CWT steelhead
        will be sacrificed for tag recovery. No steelhead evaluation is
        planned at Kooskia at this time*. Howard Burge*

3.  **Clearwater**

    1.  [Clearwater Hatchery]{.underline} - BY07 smolt release has been
        set at 843K including 360K for tribal supplementation. 1,252,900
        green eggs are requested for Clearwater Hatchery. **Table 3**.
        All spawning will occur at DNFH. Our expected first spawn date
        for Clearwater Hatchery egg collection is March 6. Spawning
        occurs on every Tuesday. When possible 1:1 male:female spawning
        will be used. On spawning days, eggs taken for CFH and Magic
        Valley will be from fresh fish that have entered DNFH trap since
        the last spawning day or fish that were green (not ripe) on
        previous spawning days and returned to the holding pond.
        Incubation to eyed stage of eggs destined for CFH production
        will occur at Dworshak Hatchery. All eggs from positive IHNV
        parentage will be culled at this point. At Dworshak Hatchery,
        the eggs will be shocked and then transferred to Clearwater
        Hatchery where they will be disinfected and placed in Heath egg
        trays. They will be picked and enumerated the next day. The eggs
        will then be placed in Heath egg trays for the remaining
        incubation period. The fry remain in the indoor vats until they
        are approximately 35 fish per pound. Each vat is loaded with
        approximately 45k swim-up fry*. Jerry McGehee*

    2.  [Magic Valley]{.underline} *-* 1,514,200 green eggs are
        requested for Magic Valley and Hagerman NFH. **Table 3**. Our
        expected first spawn date for these hatcheries is March 21. Eggs
        are taken to CFH Isolation Incubation each spawning day where
        they are held until certification of disease status. The
        isolation incubation building will be used to house and incubate
        the Dworshak B strain steelhead eggs destined for Magic
        Valley/Hagerman NFH. Eggs will be received on three different
        spawning days and held until the fish pathology lab determines
        virus results. Each female will be tested for viral replicating
        agents. At that time, positive IHNV eggs will be destroyed and
        the negative will be picked, enumerated, and shipped to Magic
        Valley. *Jerry McGehee*

    3.  [Fish health]{.underline} -- Each female spawned at Dworshak NFH
        (eggs to be reared at Clearwater Hatchery) will have either
        ovarian fluid sample or kidney/spleen tissue samples taken,
        shipped to Eagle Fish Health Lab, and tested for viral
        replicating agents; only negative tested eyed eggs are
        transferred to Clearwater Fish Hatchery main incubation for
        rearing at CFH. Tissues samples (kidney/spleen) will be from at
        least 30 females. All eggs from virus positive females will be
        culled from production. Juvenile rearing inspections will be
        performed each quarter and diagnostic examination on demand by
        Eagle Fish Health Lab. Pre-liberation inspections will also be
        performed on a 20 fish sample 10 to 15 days before liberation.
        No prophylactic treatments are planned at this time. *Doug
        Munson*

    4.  [Marking plans]{.underline} - Plans for BY07 steelhead from
        Clearwater hatchery are found in **Table 4**. As fish are moved
        outside, they receive ad-clips, ventral fin clips, and test
        groups receive CWT's. Fish will remain there until they are full
        smolt size and age, maximum of 4.5 to 6.0 fish per pound.
        (Raceways are loaded with approximately 50,000 -70,000 fish). In
        February or March, 300 fish from each release group are injected
        with PIT tags. *Tom Rogers*

4.  **South Fork Un-clipped Program**

    1.  [Planned rearing / juvenile marking & tagging]{.underline} --
        See Dworshak NFH and Clearwater FH for information.

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2.  **SPRING CHINOOK SALMON**

> *The total adult return goal for Dworshak NFH and Clearwater Hatchery
> is 21,135 spring Chinook over Lower Granite Dam. Currently Kooskia NFH
> and NPTH do not have established adult return goals. Broodstock needs
> for all facilities total 4,610 adults, specifically: 1,200 for
> Dworshak, 800 for Kooskia, 1,860 for Clearwater, 750 for NPTH.
> Additional details are listed in the pertinent sections below.*

1.  **Broodyear 2005 Spring Chinook**

    1.  **Dworshak** -- *Approximately 1,200 Chinook are needed for
        broodstock for the Dworshak spring Chinook salmon program. This
        number includes jacks and accounts for pre-spawning mortality.
        This brood level will provide 1.5 million green eggs and 1.05
        million smolts at an average of 89% eyed egg-to-smolt*

> *survival to meet the adult return goal of 9,135 to the river above
> Lower Granite Dam.*

1.  [Production status]{.underline} - On December 1, 2006, there were
    968,465 BY05 spring Chinook averaging 34 fpp and 117 mm (4.6 inches)
    total length on station. At present, these fish appear to be on
    schedule to meet the size-at-release requirements of 18--20 fish per
    pound. *Thomas Trock*

2.  [Projected release]{.underline} -- In March 2007, projected release
    will be approximately 960,000 spring Chinook. Chinook will be
    released (forced out of raceways) on two consecutive evenings from A
    and B banks in mid to late March with a number of environmental
    factors considered: flows, turbidity, increasing hydrograph, and new
    moon -- to give fish as much cover from predators as possible.
    *Thomas Trock* (**Table 5**)

3.  [Fish health]{.underline} - BY05 SCS have done very well to date. No
    treatments have been required and there is no sign of IHNV. Monthly
    monitoring samples for BKD are currently being taken. A pre-release
    exam of 60 fish will be sampled for viral and bacterial pathogens
    prior to release. *Kathy Clemens*

4.  [M&E]{.underline} - Approximately 130,000 Dworshak stock are CWT for
    system contribution monitoring. Prior to release 500 marked fish
    from each coded-wire tag code are checked for tag retention (BY04 =
    99.3 %). 52,000 Dworshak spring Chinook salmon are PIT tagged by the
    FWS Columbia River Fisheries Program Office (Vancouver) for
    Dworshak's contribution to the Comparative Survival Study (CSS).
    \[BY04 = 54.6%, 35.3 days\] and BioMark will likely PIT tag another
    53,000 Chinook for a COE/USGS study. *Howard Burge / Jill Olson /
    Ralph Roseberg*

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2.  **Kooskia** - *Approximately 800 Chinook are needed for broodstock
    for the Kooskia spring Chinook salmon program. This number includes
    jacks and accounts for pre-spawning mortality. This brood level
    produces 600,000 smolts at an average 80% eyed egg-to-smolt
    survival.*

    1.  [Production status]{.underline} - There are about 391,698
        Kooskia and 178,943 Dworshak stock spring Chinook at Kooskia for
        a total of 570,641 BY05 fry weighing 16,006 lbs, 4.31 inches or
        110 mm long, at 35.7 fish/lb (fpp). The Burrows ponds were put
        on Clear Creek water October 10 2006. Chinook will be split from
        Burrow's ponds into raceways late January, 2007. *Adam Izbicki*

    2.  [Projected release]{.underline} - KNFH will release both stocks
        on station for an approximate total of 570,000 spring Chinook
        (178,500 Dworshak and 391,500 Kooskia stock) at 20-25 fpp on or
        after the last week in March. (**Table 5**) *Adam Izbicki*

    3.  [Fish health]{.underline} -- Treated for *Ich* in late
        summer 2005. Monthly monitoring samples for BKD are currently
        being taken. A sample of 60 fish will be taken and assayed for
        virus, bacteria, and parasites prior to release. *Marilyn Blair*

    4.  [M&E]{.underline} -- Approximately 178,500 Dworshak stock are
        CWT for production contribution monitoring and stock
        identification upon return. In 2009 all 2-ocean adult returnees
        with CWT will be identified as Dworshak stock Chinook, which
        will allow separation of stocks at Kooskia for management
        purposes. Prior to release 500 marked fish from each mark group
        (tag code) are checked for tag retention \[BY04 = 51.5%, 34.9
        days\]. 10,000 Kooskia stock Chinook will be PIT tagged for the
        2007 release for juvenile and adult monitoring. The last ISS
        release was in 2004 and returns from that group will be complete
        in 2007. The plan is to continue monitoring until 2009. *Howard
        Burge*

3.  **Clearwater -** *Approximately 1,860 Chinook are needed for
    broodstock for the Clearwater Fish Hatchery spring Chinook salmon
    program. This number includes 1,020 for Powell, 840 for the SF
    program and also accounts for pre-spawning mortality. Original
    design memorandum shows the production goal may be as high as three
    million Chinook smolts. Historically, Chinook releases from
    Clearwater have ranged up to approximately 1.98 million smolts,
    1.65million presmolts and 1.0 million parr. Adult return goal for
    the program is 12,000 adult Chinook over Lower Granite Dam.*

    1.  [Production status/transfer date/projected release]{.underline}
        -- Fish will be released from transportation trucks at
        designated release sites. The release number is determined by
        subtracting fish loss from the inventory at the time of Ad
        clipping. Red River, Powell, and Crooked River acclimation ponds
        will be watered up and screens put in place by the third week of
        March. Fish will be transported to the facility and placed in
        the pond during the last week of March to first week of April.
        Smolts are then released directly from the ponds between April
        10 and 15. At Crooked River smolts will be released directly
        from the ponds between April 7 and 15 or daily if intake ice-up
        problems are anticipated. Due to unknown causes, a significant
        mortality has occurred to smolts somewhere between the upper and
        lower facilities during the spring of 2004 and 2005. During
        Spring 2007, 80% of the Crooked River full-term smolts will be
        direct released at the lower facility. All production Chinook
        are Ad clipped. During the first week of April (dates) the NPT
        will transport approximately 262,500 smolts to the Selway River
        for release near the mouth of Meadow Creek. Transport should be
        coordinated with Butch Harty and Mike Key. Planned releases of
        BY04 spring Chinook smolts are for 1,912,000 fish at an expected
        16-20 fish per pound (115,879 pounds of fish). (**Table 5**)
        *Jerry McGehee*

    2.  [Fish health]{.underline} -- [Brood Powell Spring
        Chinook]{.underline}: IHNV was detected in 14/60 pools (3 fish
        per pool) of ovarian fluids (61 fish sampled). These positive
        detections were reported to the APHIS veterinarian-in-charge.
        ELISA sampling detected 3 Highs (3.7%) out of 81 fish sampled.
        Eggs from females with high ELISA values were culled from the
        Clearwater Chinook salmon program. Prespawning mortality was at
        2%.

> [Broodyear 2005 Powell]{.underline}: Pathogens have not been detected
> in these fish to date during routine sampling.
>
> [Brood S. F. Clearwater Spring Chinook]{.underline}**:** IHNV was
> detected in 14/48 pools (3 fish per pool) of ovarian fluids. These
> positive detections were reported to the APHIS veterinarian-in-charge.
> ELISA sampling detected 3 Highs (8.9%), out of the 126 females
> sampled. Eggs from females with
>
> high ELISA values were culled from the Clearwater Chinook salmon
> program. Prespawning mortality was at 4%.
>
> [Broodyear 2005 S. F. Clearwater Spring Chinook]{.underline}**:** No
> pathogens have been detected during routine sampling.

######## [Juvenile]{.underline}

-   Rearing inspections -- Quarterly inspections are performed by Eagle
    Fish Health Lab. No pathogens detected in regular monthly
    inspections except for 1 DFAT positive for Renibacterium
    salmoninarum in the South Fork of the Clearwater spring Chinook
    salmon.

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-   Pre-liberation inspections -- These inspections are performed by
    Eagle Fish Health Lab within 45 days of release.

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-   Pre-emptive -- 2 Erythromycin medicated feed treatments throughout
    rearing cycle.

-   Nine raceways received 1 treatment as part of University of Idaho
    and IDFG Research.

-   Quarterly inspections. Pre-liberation prior to release at Satellites
    (20 fish samples). *Doug Munson*

    1.  [M&E]{.underline} *-* The fish are sampled monthly between the
        25th and 28th of the month. During months of rapid growth, fish
        are sampled biweekly. Pound counts are taken to track fish
        growth and monitor if growth is following the annual growth
        projections. Length frequencies are taken monthly. Approximately
        30 days prior to release, 100 fish are sampled to quality check
        Ad clips, ventral fin clips, and CWT retention. *Jerry McGehee*

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    1.  **Broodyear 2006 Spring Chinook**

        1.  **Dworshak**

            1.  [Production status]{.underline} - All of Kooskia stock
                spring Chinook eggs were shipped to Kooskia NFH during
                October, 2006. Approximately 1.05 million of Dworshak
                stock remained at Dworshak for incubation over the
                winter. On December 1, 2006, there were approximately
                1.09 million sac-fry incubating at Dworshak. In the
                spring of 2007, Dworshak stock will be ponded directly
                into raceways at Dworshak. *Thomas Trock*

            2.  [Fish health status]{.underline} - BY06 has experienced
                no problems to date. 60 fish will be sampled prior to
                release. *Kathy Clemens*

            3.  [M&E]{.underline} Approximately 130,000 Dworshak stock
                were CWT in August for contribution monitoring (**Table
                6**). Tagging plans also include 52,000 PIT tags for the
                Comparative Survival Study (CSS). The CSS is looking at
                adult survival of transported vs. non‑transported and
                up‑river vs. down‑river releases. Also another 53,000
                Chinook will be PIT tagged for a COE funded / NMFS study
                looking at latent mortality associated with barge and
                in-river life-history strategies. *Howard Burge / Jill
                Olson*

        2.  **Kooskia**

            1.  [Production status]{.underline} - Kooskia stock BY06
                spring Chinook eggs were taken from a total of 252
                females spawned with a total of 155 males. This produced
                a total of 882,000 green eggs. All of the Kooskia stock
                were transferred to KNFH between October 16 -- 20. Eggs
                were incubated on chilled well water, at temperatures of
                approximately 39F. Normally eggs all hatch out by mid
                January. The eggs will be incubated on chilled well
                water to prevent silt problems due to spring runoff and
                to protect the sac fry from ICH until tanking and
                feeding in late March. Approximately 600k Kooskia stock
                will be reared to \~25-20 fpp. These fish will be
                released April 2008. *Adam Izbicki*

            2.  [Fish health status]{.underline} - BY06 has experienced
                no problems to date, 60 fish will be sampled prior to
                release. *Marilyn Blair*

            3.  [M&E]{.underline} - Adult monitoring for the ISS will
                continue, as will monitoring of the Kooskia weir.
                Approximately 100,000 fish will be CWT in August for
                contribution (**Table 6**) and 10,000 smolts will
                receive PIT tags prior to release. *Jill Olson / Ralph
                Roseberg / Howard Burge*

        3.  **Clearwater**

            1.  [Production status]{.underline} -- The proposed number
                of Clearwater Fish Hatchery fish to be allocated from
                brood year 2006 is 1.970 million smolts, 503K
                pre-smolts, and 304K parr. *Jerry McGehee*

            2.  [Estimated numbers/planned marking &
                tagging]{.underline} - All production Chinook are Ad
                clipped. Planned releases of BY06 Chinook are for
                1,970,000 smolts 15-20 fish per pound. (This includes
                125K that will be transferred to NPTH in Sept 2007.)
                503,000 pre-smolts 20-25 fpp and 307K parr 30-50 fpp
                will be released from transportation trucks at
                designated release sites. Red River, Powell, and Crooked
                River acclimation ponds will be watered up and screens
                put in place by the third week of March each year. Fish
                will be transported to the facility and placed in the
                pond during the third week of March. Smolts are then
                released directly from the ponds between April 1 and 10.
                The NPT will transport and release 307,000 smolts
                directly into the lower Selway River near the mouth of
                Meadow Creek. (**Table 6**) *Tom Rogers*

            3.  [Fish health status]{.underline} -- [Brood Powell Spring
                Chinook]{.underline}**:** IHNV was detected in 24/60
                (sampled individually) of ovarian fluids and
                kidney/spleen tissues . These positive detections were
                reported to the APHIS veterinarian-in-charge. ELISA
                sampling detected 3 Highs (1.25%) of the 240 females
                spawned. Eggs from females with high ELISA values were
                culled from the Clearwater Hatchery Chinook salmon
                program. Prespawning mortality was at 5% in 2005.

> [Brood S.F. Clearwater Spring Chinook]{.underline}**:** IHNV was
> detected in 24/60 of ovarian fluids and kidney/spleen tissues. These
> detections were reported to the APHIS veterinarian-in-charge. ELISA
> sampling detected 20 Highs (3.5%) of the 574 females sampled. Eggs
> from females with high ELISA values were culled from the Clearwater
> Hatchery Chinook program. Prespawning mortality was at 5% in 2005.
>
> [Eggs- Disease Sampling]{.underline}: When the females are spawned,
> kidney samples are collected from all females; ovarian samples are
> collected from 60 and kidney/spleen tissues from at least 30 females
> (viral replicating agent analysis) as well as head wedges from 20 fish
> for disease testing. All samples are air freighted weekly to the Eagle
> Fish Health lab for analysis. Females are screened for BKD using ELISA
> techniques. Females with optical densities (OD) over 0.25 are culled.
>
> [Juvenile]{.underline}

-   Rearing inspections -- quarterly inspections are performed by Eagle
    Fish Health Lab

-   Pre-liberation inspections -- These inspections are performed by
    Eagle Fish Health Lab

-   Pre-emptive -- 1 Erythromycin medicated feed treatment throughout
    rearing cycle.

-   Some raceways will receive 3 treatments as part of University of
    Idaho and IDFG Research because of BKD moderate group being reared.

-   Quarterly inspections. Preliberations prior to release at Satellites
    (20 fish samples). *Doug Munson*

    1.  [M&E]{.underline} - The fish are sampled monthly between the
        25th and 28th of the month. During months of rapid growth, fish
        are sampled biweekly. Pound counts are taken to track fish
        growth and monitor if growth is following the annual growth
        projections. Length frequencies are taken monthly. Approximately
        30 days prior to release, 100 fish are sampled to quality check
        Ad clips, ventral fin clips, and CWT retention. *Chris
        Harrington*

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    1.  **Nez Perce Tribal Hatchery** *Approximately 750 spring Chinook
        salmon adults are needed for broodstock for the Nez Perce Tribal
        Hatchery spring Chinook program. This number includes jacks and
        accounts for pre-spawning mortality. This brood level will
        provide for a target release of 75,000 presmolts from Newsome
        Creek (South Fork Clearwater River) acclimation facility,
        150,000 presmolts from Yoosa/Camp (Lolo Creek) acclimation
        facility and 400,000 parr into Meadow Creek (Selway River).*

        1.  [Production status]{.underline} -- A total of 246 spring
            Chinook were trapped at Nez Perce Tribal Hatchery facilities
            in 2006: Lolo Creek (117 adults), Newsome Creek (77 adults,
            2 jacks), Site 1705 (48 adults, 2 jacks). In addition, 20
            adults were obtained from Dworshak for backup broodstock. Of
            these, 89 females were spawned: Lolo Creek (41), Newsome
            Creek (22), DNFH (10) and Site 1705 (16) and yielded
            approximately 317,045 green eggs. Throughout all phases of
            incubation, water temperatures were controlled to meet the
            following temperature regime: Aug. 10^th^ - 9º C (48.2º F);
            Sept. 15^th^ -- 6.5º C (43.7º F); Sept. 30^th^ -- 6.0º C
            (42.8º F); Oct. 15^th^ thru Feb. 15^th^ -- 4.0º C (39.2º F).
            At approximately 600 temperature units (TU's) eggs are
            shocked and machine sorted the following day. As of December
            31, 2006, a total of 301,588 sac fry were on hand at NPTH.
            In September 2007, 125,000 juveniles will be transferred
            from Clearwater Hatchery to the acclimation ponds at
            NPTH/Site1705. The smolts will be released directly from the
            ponds into the Clearwater River in late March or early
            April 2008. Target size at release is 18 fish per pound
            (25.2 g.) Targeted 2007 releases: (**Table 5**) *Butch
            Harty*

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-   150,000 presmolts (acclimated) into Yoosa/Camp/Lolo Creek in October

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-   75,000 presmolts (acclimated) into Newsome Creek in October

-   40,000 parr (direct stream) into Meadow Creek (lower) in June

    1.  [Estimated numbers/planned marking & tagging]{.underline} --
        Fish destined for release from acclimation facilities will be
        marked (CWT) at approximately 180 fish per pound (2.52 g) in
        late April to early May, held in production room tanks or
        raceways at NPTH. Post marking, the Newsome Creek fish will be
        transferred to Sweetwater Springs held until early September and
        then transferred to the Newsome Creek AF for acclimation and
        final rearing. Lolo Creek fish will be held at NPTH until late
        August and then transferred to Yoosa/Camp AF for acclimation and
        final rearing. *Butch Harty*

    2.  [Acclimation facility operations/release]{.underline} --

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-   Yoosa/Camp -- Yoosa/Camp -- Transfer of the fish will occur in late
    August (when water temperatures cool). Facility will be set-up and
    operational at least 2 days prior to transfer of fish. Prior to
    release, 9,000 fish will be tagged with a PIT tag. Volitional
    release will commence on October 2, with all fish forced out by
    October 16, 2007. Target size at release is 34 fish per pound (13.3
    g). **(Table 5)**.

-   Newsome Creek -- After marking, fish will be transferred to the
    Sweetwater Spring facility, held until late August/early September
    (when water temperatures cool) and then be transferred to the
    Newsome AF facility. Prior to release, 6,000 fish will receive a PIT
    tag. Volitional release will commence October 3, with all remaining
    fish forced out by October 17, 2007. Target size at release is 29
    fish per pound (15.6 g). **(Table 5)**.

    -   Meadow Creek -- Due to broodstock limitations, there will be
        only 40,000 parr for release into Meadow Creek in 2007. Prior to
        release, 5,000 fish will receive a PIT tag. Fish will be direct
        stream released into lower Meadow Creek at Slims Camp on June
        15, 2007. Target size at release is 117 fish per pound (3.9
        grams). **(Table 5)**. *Butch Harty*

    4.  [Fish health status]{.underline} -- There were no viruses
        isolated during spawning from any of the NPT SCS sites. No eggs
        were culled because they had a high ELISA value. *Kathy Clemens*

    5.  [M&E]{.underline} -

        -   Tag retention and delayed mortality -- Estimate CWT delayed
            mortality rates within 5 days of tagging. Estimate CWT
            retention rates 25-35 days after tagging. Estimate PIT tag
            retention rates and delayed mortality within 5 days of
            tagging.

        -   PIT survival studies- Estimate smolt survival rates and
            migration timing **(Table 6)***.*

        -   Downstream migration -- Operate rotary screw traps within
            Meadow Creek, Lolo Creek and Newsome Creek to monitor
            movement, timing, condition factors, and population
            estimates. *Sherman Sprague*

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    6.  [Communication]{.underline} - NPTH produces monthly production
        and pathology reports. Fish Research produces weekly weir
        reports, final weir summary report, spawning ground summary
        reports, and SURPH survival summary reports.

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    2.  **Broodyear 2007 Spring Chinook**

        1.  **Dworshak**

            1.  [Projected adult returns]{.underline} - Based on 2006
                draft tribal harvest, sport harvest data, and rack
                returns; the expected return for BY07 adult spring
                Chinook to North Fork Clearwater is 1,641 fish. Our
                forecast for the 2007 spring Chinook salmon returns to
                Dworshak NFH is given in **Table 7a**. FWS is optimistic
                that they will meet broodstock requirements. IDFG and
                the NPT may decide to open sport and tribal fisheries in
                the Clearwater River in the spring of 2007 after dam
                counts of PIT tagged adults possibly revise our
                estimates. *Jill Olson / Ralph Roseberg*

            2.  [Ladder operation]{.underline} -- Ladder opening will be
                influenced by possible fisheries openings and in-season
                run projection adjustments. If fisheries are open and
                the run looks good the ladder may be opened around the
                first of June to collect \~200 Chinook and then closed
                until July, when regular spring Chinook trapping would
                begin. If the run estimate is close to our prediction or
                total brood needs we will likely open the ladder the end
                of June and leave it open to maximize collection of
                Dworshak broodstock and brood for other facilities. The
                NPT requested that adult spring Chinook in excess of
                DNFH broodstock requirements be provided for broodstock
                at NPTH. The Service will notify Butch Harty and Becky
                Johnson. *Howard Burge*

            3.  [Adult outplanting / distribution plans]{.underline} --
                **Table 8a** list the priority of streams to receive
                adult spring Chinook salmon. Outplanting will be
                coordinated between Mike Key (NPT) and Jill Olson (FWS).
                All adults ouplanted from Dworshak will receive a left
                opercle v-notch as shown in **Table 8b**. *Howard Burge
                / Jill Olson / Ralph Roseberg*

            4.  [Adult M&E]{.underline} Returning adults are measured
                and examined for gender, various clips and tags, and
                seal bites or other injuries, then sorted for spawning
                or holding. *Jill Olson / Ralph Roseberg*

            5.  [Spawning plans]{.underline} Dworshak will spawn 350-425
                females for its program and 250-300 females for
                Kooskia's program. *Thomas Trock*

            6.  [Egg Incubation]{.underline} All eggs taken for Kooskia
                and Dworshak will be initially incubated at Dworshak.
                After eye-up and enumeration, all of Kooskia eggs will
                be shipped to Kooskia for final incubation. All of
                Dworshak eggs will be incubated at Dworshak. *Thomas
                Trock*

            7.  [Fish health]{.underline} -- Every adult female will be
                sampled individually for BKD with ELISA. Up to 150
                ovarian fluid samples (3 pool) will be sampled for
                viruses. An additional 60 tissue samples will be taken
                for bacteria assays,

> and 20 samples for *Myxobolus cerebralis and C. Shasta*. Eggs from
> high and medium ELISA level females will be culled; exact level will
> depend upon number of fish returning. *Kathy Clemens*

8.  [Communication]{.underline} FWS puts out weekly spawning reports and
    weekly return reports, and annual spawning and adult return reports
    are also produced.

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1.  **Kooskia**

    1.  [Projected adult returns]{.underline} - Based on 2006 draft
        tribal harvest, sport harvest data, and rack returns; Kooskia's
        BY07 adult Chinook return is expected to be about 400 fish. Our
        forecast for the 2007 spring Chinook salmon returns to Kooskia
        NFH is given in **Table 7a**. FWS is not optimistic that they
        will meet their broodstock requirements of 800 Chinook. *Jill
        Olson / Ralph Roseberg*

    2.  [Trap operation]{.underline} -- Trap will be opened for Chinook
        collection around the beginning of May. All natural and
        returning ISS 3-ocean adults (up to 60 pairs of unmarked/ventral
        clip adults) will be released upstream for natural spawning in
        accordance with ISS protocol. ISS adults in excess of what is
        needed for natural spawning will be incorporated into general
        production. Adults collected for broodstock will be transported
        to Dworshak for holding until spawning. *Howard Burge / Jill
        Olson*

    3.  [Adult outplanting / distribution plans]{.underline} - **Table
        8a** list the priority of streams to receive adult spring
        Chinook salmon. Chinook loaded for adult outplanting will be
        loaded directly into NPT trucks at Kooskia. Outplanting will be
        coordinated between Mike Key (NPT) and Jill Olson / Ralph
        Roseberg (FWS). All adults ouplanted from Kooskia will receive
        two right opercle v-notchs as shown in **Table 8b**. Tribal use
        of un-anesthetized jacks for the elder program will need to be
        coordinated prior to adult sorting. (NPT contact Nancy
        McAllaster, 208-843-7320 ext.2445) *Jill Olson / Ralph Roseberg*

    4.  [Adult M&E]{.underline} Returning adults are measured and
        examined for gender, various clips and tags, and seal bites or
        other injuries, then sorted for spawning or holding. We also
        hope to have a PIT tag detector installed and operational on the
        adult trap intake by April. *Jill Olson / Ralph Roseberg*

    5.  [Spawning plans]{.underline} - Kooskia spring Chinook adult
        broodstock will be kept at Dworshak NFH. Spawning normally
        occurs the third week of August. Eggs collected that are in the
        low range of the BKD testing will be kept and the medium to high
        eggs are discarded. Jacks will be utilized for \~10% of the
        spawners. *Adam Izbicki*

    6.  [Egg incubation]{.underline} - BY07 Kooskia stock (750k) eggs
        will be transferred to KNFH beginning of November after eye-up.
        Eggs will be incubated on chilled well water, approximately
        38-40F. Normally eggs all hatch out by mid January and are
        tanked mid March. *Adam Izbicki*

    7.  [Fish Health]{.underline} - Every adult female will be sampled
        individually for BKD with ELISA. Up to 150 ovarian fluid samples
        (3 pool) will be sampled for viruses. An additional 60 tissue
        samples will be taken for bacteria assays, and 20 samples for
        *Myxobolus cerebralis and C. shasta.* Eggs from high and medium
        ELISA level females will be culled; exact level will depend upon
        number of fish returning. *Kathy Clemens*

    8.  [Communication]{.underline} - FWS puts out weekly spawning
        reports and weekly return reports, and annual spawning and adult
        return reports are also produced.

2.  **Clearwater**

    1.  [Projected adults returns]{.underline} -- IDFG does not make
        formal pre-season forecasts of returns to the satellite
        facilities. The Technical Advisory Committee of US v Oregon has
        projected returns of Snake River spring/summer Chinook at Lower
        Granite Dam to be about 18% lower than the 2006 pre-season
        forecast and 15% lower than the actual numbers that returned
        in 2006. Nevertheless, depending upon in-season assessments of
        overall run strength and returns to specific hatcheries based on
        analyses of counts and PIT tag detections at dams, IDFG may
        still seek sport harvest seasons. The State sport fishery will
        be managed to stay within allowable incidental take of ESA
        listed populations and for 50% of the harvestable share of adult
        spring Chinook. Real time predictions will be used to adjust the
        share. *Tom Rogers, Ed Schriever*

    2.  [Trapping operations at satellite facilities]{.underline} -
        Spring Chinook will be trapped at the Crooked River and Red
        River weirs, which will be installed approximately the third
        week of March, prior to high water. Trapping operations will
        continue until after September 1 and five consecutive days of
        zero fish are trapped. Proposed adult needs will be
        approximately 930 females and 930 males for Clearwater Hatchery
        allocations. NPT requested adult spring Chinook in excess of
        Clearwater broodstock requirements be available for broodstock
        at NPTH. Notify Butch Harty and Becky Johnson. A minimum of 200
        females and 200 males will be requested to fill broodstock needs
        at NPTH. If CFH manager predicts elevated prespawning mortality
        in holding adults, hatchery manager will compensate for loss by
        taking and holding additional adult fish. If by commencement of
        spawning too many adults have been taken, then adult outplants
        will be implemented at locations and priorities given in **Table
        8a**. *Jerry McGehee*

    3.  [Adult outplanting / distribution plans]{.underline} - The
        outplanting protocol \[for excess hatchery broodstock\] provides
        for distribution for natural spawning and subsistence use. If
        adult Chinook, available for release into natural spawning
        areas, exceed the numbers agreed to in **Table 8a**, further
        consultation will occur. The general procedure for providing
        fish for subsistence will be first to tribal programs, then to
        charitable organizations. Jack Chinook may go to subsistence
        programs directly. Please see **Tables 8a** and **8b** for
        outplanting priority streams and marks. *Tom Rogers*

    4.  [Spawning plans]{.underline} - Spawning ratios of 1:1 will be
        used unless the brood stock population is less than 100 females.
        If the spawning population is less than 100 females, then eggs
        from each female will be split into two equal groups. A
        different male will fertilize each group. One cup of well water
        will be added to each bucket and set aside for 30 seconds to one
        minute. The two buckets will be poured together and continued
        through the spawning process. When brood stock population is 50
        to 25 females, the eggs from each female will be split into
        three equal groups and each group fertilized by a different
        male. One cup of well water will be added to each bucket and set
        aside for 30 seconds to one minute; then all three buckets will
        be poured together. When brood stock population is 25 females or
        less, the eggs from each female will be divided into four equal
        groups, each fertilized by a separate male. The process will be
        completed as previously mentioned to finish the spawning
        process. During the entire spawning year, at least five to ten
        percent of the jacks will be used during the spawning process.
        An effort will be made to use all returning fish for spawning.
        If presented with an excess number of one sex, gametes from
        individual parents may be subdivided and each part fertilized
        with gametes with different parents. The first sort will occur
        between August 5 and 10. All females will be sorted twice per
        week, and all ripe females will be spawned each time. Spawning
        will continue until all females are spawned. NPT assistance will
        be provided when spawning Chinook for NPTH. If too many eggs are
        taken for the hatchery program, these eggs can be used to
        backfill appropriate IDFG programs, other agency programs. If
        not needed, surplus eggs will be disposed. *Jerry McGehee*

    5.  [Juvenile production]{.underline} - Original design memorandum
        shows a production goal may be as high as 1.5 million Chinook
        smolts reared at the main facility, and 1.5 million fall release
        pre-smolts reared at the three satellite facilities. *Jerry
        McGehee*

    6.  [Fish Health]{.underline} - All females will be tested by ELISA
        for Bacterial Kidney Disease (BKD). All eggs from females that
        are identified at a level of 0.25 OD or higher will be culled. A
        60 fish sample (ovarian fluids) and at least 30 kidney/spleen
        (tissue) samples will be taken for viral replicating agents. A
        20 fish sample (head wedge) will be taken for *Myxobolus
        cerebralis* analysis. Juveniles will be inspected on a quarterly
        basis. Diagnostics on demand. Pre-liberation samples prior to
        release at satellites (20 fish sample). *Doug Munson*

3.  **Nez Perce Tribal Hatchery**

    1.  [Projected adult returns]{.underline} - Projected adult returns
        (**Table 7b**) Adult return estimates to Lolo and Newsome creeks
        are 229 and 66, respectively. At the present time, there are no
        adult return estimates for Meadow Creek. The 5 year average
        capture efficiency at our Lolo Creek Weirs is 27%, for Newsome
        Creek it is 84%. The total number of returning adults we expect
        to capture at our Lolo and Newsome Creek weir sites are 62 and
        55, respectively. Broodstock needs are: 120 adults for Lolo
        Creek, 60 adults for Newsome Creek, and 400 adults for Meadow
        Creek, Selway. The broodstock needs assumes a 50:50 sex ratio.
        *Sherman Sprague*

    2.  [Trapping operations at NPTH]{.underline} -- The adult ladder
        and trap, at Nez Perce Tribal Hatchery, will be operated in 2007
        to collect spring chinook adults. Trapping operations will begin
        mid-April and continue through July 31^st^. The ladder will be
        operated intermittently to collect up to 400 adults needed for
        broodstock for the Meadow Creek, Selway program. Broodstock
        needs are based on an 85% survival from collection to spawning.
        In an attempt to select adults representatively across the
        return, the trap will be open in April and May until 34% (up to
        136 adults) of the collection goal has been retained for
        broodstock. If the collection goal is reached, the trap will be
        closed until June 1. Beginning June 1, the trap will reopen
        until 33% (up to 132 adults) of the collection goal has been
        retained for broodstock. If the goal is reached, the trap will
        be closed until July 1. Beginning July 1, the trap will reopen
        until the remaining 33% (up to 132 adults) of the collection
        goal has been retained for broodstock.

> Broodstock selection will be based on existing fin clips, marks, or
> tags. Only adipose fin clipped fish will be used as broodstock and
> will be retained at the rate described above. All natural, non-adipose
> fin clipped, known Idaho Supplementation Studies (ISS), and radio
> tagged fish will be returned to the Clearwater River and allowed to
> continue their spawning migration.
>
> An alternative broodstock source for the Meadow Creek, Selway program
> is to obtain spring Chinook broodstock from other programs. Per
> agreement with IDFG and USFWS, adults returning to Crooked River,
> Rapid River, Red River, Powell satellites and transported to
> Clearwater Hatchery as well as Dworshak Hatchery may also be used for
> broodstock. Up to 560 adults (280 females and 280 males) will be
> collected at IDFG or USFWS facilities -- provided they are available -
> and transported to Site 1705 for maturation and spawning *Becky
> Johnson*

3.  [Trapping operations at Lolo Creek and Newsome Creek]{.underline} -

> [Trapping operations on Lolo and Newsome creeks usually begin at the
> end of May, after peak flows are reached. Trapping will continue
> through September 14^th^, or until zero fish are trapped for 7
> consecutive days. Two weirs will be operated on Lolo Creek, an upper
> weir (RKM 51) and a lower weir (RKM 21).]{.mark} The predicted number
> of adults captured at our weir sites will not meet NPTH broodstock
> needs. After a through review of the situation, we recommend keeping
> all adults captured at our weir sites for broodstock. [Pass/keep
> ratios will be adjusted on a weekly basis dependent on actual
> captures. The adult weirs will also be used for escapement, estimating
> sex composition, age structure, return timing and genetic tissue
> sampling.]{.mark} The following marks/tags are proposed for
> application at NPTH trap sites on fish that are passed; Lower Lolo Cr
> = Left Operculum Punch and Left Numbered Orange Tyvek Tag; Upper Lolo
> Cr = Right Operculum Punch; and Newsome Cr Left Operculum Punch.
> Adults collected for broodstock will be held at the Yoosa/Camp and
> Newsome creek satellite facilities until water temperatures in Newsome
> and/or Yoosa/Camp creeks approach 65º. If water temperatures
>
> reach this level adults will be transferred to NPTH for maturation and
> spawning. *Becky Johnson*

4.  [Adult outplanting plans]{.underline} - Please see **Table 8a** and
    **8b**. *Becky Johnson*

5.  [Spawning plans]{.underline} -- The first sort and spawn will occur
    August 7^th^. Spawning will occur will occur on Tuesday of each week
    at NPTH (moribund ripe females will not be spawned). Spawning
    schedule: Tuesday and Wednesday. A spawning ratio of 1:1 will be
    used. Jacks will be limited to ten percent of the male contribution.
    Spawning will continue until the egg take goal is achieved or all
    females are spawned. *Butch Harty*

6.  [Juvenile production]{.underline} --

    -   Phase I production goals are 625,000 parr/pre-smolts.
        Distribution of juvenile production is 400,000 parr (Meadow
        Creek), 150,000 pre-smolts (Lolo Creek), and 75,000 pre-smolts
        (Newsome Creek). Throughout all phases of incubation, water
        temperatures will be controlled to meet the following
        temperature regime: Aug. 10^th^ - 9º C (48.2º F); Sept. 15^th^
        -- 6.5º C (43.7º F); Sept. 30^th^ -- 6.0º C (42.8º F); Oct.
        15^th^ thru Nov. 30^th^ - 4.0º C (39.2º F); Dec. 1^st^ thru Dec.
        31^st^ - 2.5º C (36.5º F).

    -   Juvenile production destined for remote sites will be held in
        production room tanks, raceways or NATURES "S" channels at Site
        1705, tanks at the Sweetwater facility and transferred when
        conditions permit (end of August to the second week of
        September. Production will be marked 100% with a CWT and
        sub-release groups will be PIT tagged. Monitor the in-hatchery
        survival and rearing conditions by treatment and rearing
        strategy. *Butch Harty*

7.  [Fish Health]{.underline} - All females will be tested by ELISA for
    Bacterial Kidney Disease (BKD). All eggs from females that are
    identified at a level of 0.20 OD or higher will be culled. A 150
    fish sample (ovarian fluids) will be taken for viral replicating
    agents. A 20 fish sample (head wedge) will be taken for *Myxobolus
    cerebralis* analysis. Juveniles will be inspected on a quarterly
    basis. Diagnostics on demand. Pre-liberation samples prior to
    release (60 fish sample). *Kathy Clemens*

8.  [Communication]{.underline} - A monthly NPTH narrative and fish
    health report will be completed and submitted to BPA/COTR, NPT
    Research and Production divisions, IDFG/Clearwater Fish Hatchery and
    all other interested parties.

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2.  **COHO -** *A coho reintroduction program was initiated by the Nez
    Perce Tribe in 1995. Recent production releases have occurred in
    Lapwai Creek (275,000 smolts), Potlatch Creek (275,000 smolts),
    Clear Creek (acclimated at Kooskia -- 280,000 smolts), and*
    *Eldorado Creek, Lolo Creek, and Musselshell Creek (total 270,000
    pre-smolts). Fish production for this program comes from Eagle Creek
    NFH, Dworshak, and Clearwater hatcheries.*

    1.  **Broodyear 2005 Coho**

        1.  **Dworshak**

            1.  [Production status]{.underline} -- There were 295,915
                fish on hand (4.87", 11,797 pounds, 24.9 fpp) at
                Dworshak as of December 28th, 2006. *Ed Larson*

            2.  [Projected transfer date/acclimation period at
                Kooskia]{.underline} -- Smolts will be transferred to
                Kooskia NFH as soon as Kooskia spring Chinook are
                released in April 2007 for a 3-5 week acclimation. *Ed
                Larson*

            3.  [Numbers/dates/marks & tags]{.underline} - 87,807
                fingerling coho were marked with a CWT (no AD clip) on
                July 10th, 2006. 1,500 fish will be PIT tagged at
                Kooskia as soon as fish are there for acclimation in
                April 16^th^-20^th^, 2007. (**Table 9**) *Ed Larson*

            4.  [Fish health]{.underline} -- Disease history for this
                broodyear of fish is complete at Lower Columbia River
                Fish Health Center for eggs that are transferred in. All
                fish are certified disease free. Fish are sampled no
                less than quarterly and prior to liberation; a 60 fish
                sample will be taken and assayed for virus, bacteria,
                and parasites. Fish were treated with Florfenicol
                medicated feed under INAD prior to transfer to Kooskia
                to help guard against mortality from Bacterial Coldwater
                Disease. *Kathy Clemens*

            5.  [Juvenile M&E]{.underline} --

                -   Juvenile survival and emigration timing to Lower
                    Granite Dam.

                -   Smolt-to-adult survival, and adult return timing
                    based on counts at Lower Granite Dam and ladder
                    counts at Dworshak and Kooskia National Fish
                    Hatcheries. *Ed Larson*

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        1.  **Transfers (Eagle Creek NFH)**

            1.  [Projected release]{.underline} - Smolts reared at Eagle
                Creek NFH will be released into Clear Creek, M.F.
                Clearwater River and Lapwai Creeks March 5^th^ through
                9^th^, 2007. Approximately 550,000 (275,000 each stream)
                will be direct released. Approximately 25,000 will be
                CWT/AD, and 25,000 will be CWT only per release site.
                There will be 1,500 PIT in each release group to assess
                survival to Lower Granite Dam. (**Table 9**) *Ed Larson*

            2.  [Fish health]{.underline} -- Disease history for this
                broodyear of fish is complete at Lower Columbia River
                Fish Health Center. All fish are certified disease free.
                *Kathy Clemens*

            3.  [M&E]{.underline}

                -   Juvenile survival and emigration timing to Lower
                    Granite Dam.

                -   Smolt-to-adult survival and adult return timing
                    based on counts at Lower Granite Dam.

                -   Genetic samples collected for future analysis. *Ed
                    Larson*

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    1.  **Broodyear 2006 Coho**

        1.  **Dworshak**

            1.  [Production status]{.underline} - Adults recognized at
                Lower Granite Dam totaled 1,141 and 293 jacks in 2006. A
                total of 437 coho salmon broodstock (188 females, 193
                males, 41 jacks, 2 jills and 15 pre-spawn mortalities)
                were collected from Dworshak and Kooskia NFH's, Nez
                Perce Tribal Hatchery, and Lyons Ferry FH. Total green
                egg collection of 399,447 resulted in

            2.  281,837 eyed eggs from adults designated as Clearwater
                stock. On December 8th, 2006 an additional 170,000 eyed
                eggs were transferred from Eagle Creek NFH. On December
                9th - 30,000 eggs were transferred to the Potlatch Corp
                Union Worker personnel (Owen Squires). As of January
                9th, 2007, at Dworshak NFH a total of 418,925 fry
                (253,197 Clearwater stock, 165,728 Eagle Creek stock)
                are in incubation and the nursery. *Ed Larson*

            3.  [Projected production]{.underline} - The projected
                production will be 280,000 smolts reared through
                spring 2008. 100,000 of these fish will receive CWT and
                1,500 will receive PIT tags. (**Table 10**) *Ed Larson*

            4.  [Fish health]{.underline} -- Every adult female was
                sampled individually for BKD with ELISA; low values
                resulted in no eggs culled. Up to 150 ovarian fluid
                samples (3 pool) were sampled for viruses; no viruses
                were detected. An additional 60 tissue samples were
                taken for bacteria assays, and 60 samples for *M.
                cerebralis.* Brood fish health samples were taken by NPT
                staff and delivered to Idaho Fish Health Center
                personnel at Eagle, Idaho for analysis. Juvenile fish
                will be sampled quarterly and prior to liberation. We
                suggest treating with Florfenicol prior to transfer to
                Kooskia to help guard against post-transport, stress
                induced mortality from Bacterial Coldwater Disease.
                *Kathy Clemens*

            5.  [M&E]{.underline} - Juvenile survival and emigration
                timing to Lower Granite Dam. Smolt-to-adult survival,
                and adult return timing based on counts at Lower Granite
                Dam and ladder counts at Dworshak and Kooskia National
                Fish Hatcheries. Genetic samples collected from adults
                for future analysis. *Ed Larson*

        2.  **Clearwater**

            1.  [Production status]{.underline} - Due to funding cuts in
                the Pacific Coastal Salmon Recovery Fund, the Nez Perce
                Tribe was forced to discontinue coho salmon production
                at Clearwater Hatchery. No eggs were allocated for this
                production from BY06. *Ed Larson*

        3.  **Transfers (Eagle Creek NFH)**

            1.  [Projected release]{.underline} - Smolts reared at Eagle
                Creek NFH will be released into Clear and Lapwai Creeks
                in mid-March 2007/08. Approximately 550,000 (275,000
                each stream) will be direct stream released.
                Approximately 25,000 will be CWT/AD, and 25,000 will be
                CWT only per release site. There will be 1,500 PIT in
                each release group. *Ed Larson*

            2.  [Fish health]{.underline} -- Disease history for this
                broodyear of fish is complete at Lower Columbia River
                Fish Health Center. All fish are certified disease free.
                *Kathy Clemens*

            3.  [M&E]{.underline}

                -   Juvenile survival and emigration timing to Lower
                    Granite Dam.

                -   Smolt-to-adult survival and adult return timing
                    based on counts at Lower Granite Dam and weir
                    monitoring at Lapwai Creek and the Potlatch River,
                    and redd surveys.

                -   Genetic samples collected for future analysis. *Ed
                    Larson*

    2.  **Broodyear 2007 Coho -** *A primary program objective is to
        develop a local Clearwater River Coho stock. To accomplish this
        adult Coho returning to the Snake River basin and Clearwater
        River are a priority for use as broodstock. Fish are collected
        at fish ladders at Dworshak NFH, Kooskia NFH, Lyons Ferry FH,
        Nez Perce Tribal Hatchery and two weirs. In 2007, there will be
        insufficient funds to operate weirs on the Potlatch River and
        Lapwai Creek for monitoring and broodstock collection;
        therefore, the Potlatch smolt release will be transferred to
        Clear Creek to facilitate future broodstock collection at
        Kooskia NFH.*

        1.  **Dworshak**

            1.  [Ladder operation]{.underline} - Ladder operation will
                begin on October 1, 2007 to begin trapping steelhead and
                Coho salmon at Dworshak and Kooskia NFH. NPT requests
                that the Dworshak ladder be operated such that sorting
                can occur twice a week in order to maximize the adult
                take. *Howard Burge*

            2.  [Adult transfers]{.underline} - Adult steelhead trapped
                (in excess of 500 fish) during operation of the Dworshak
                ladder to collect Coho will be loaded and hauled by NPT
                for release in the lower Clearwater River at Hog Island.
                *Ed Larson*

            3.  [Juvenile M&E]{.underline} --

                -   Smolt-to-adult survival based on weir monitoring in
                    Lapwai Creek and the Potlatch River will not occur
                    unless funding is restored. Limited redd surveys may
                    occur in Lapwai Creek and the Potlatch River.

                -   Smolt-to-adult survival and adult return timing
                    shall be based on counts at Lower Granite Dam and
                    ladder counts at Dworshak and Kooskia NFH, Lyons
                    Ferry Hatchery, Nez Perce Tribal Hatchery.

                -   Genetic samples collected from adults. *Ed Larson*

            4.  [Communication]{.underline} - NPTH produces monthly
                production and pathology reports, and Fish Research
                produced weekly weir reports (prior to funding cuts).
                *Ed Larson*

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3.  **FALL CHINOOK SALMON -** *Fall Chinook salmon production in the
    Clearwater River occurs through two programs -- Lower Snake River
    Compensation Plan/Fall Chinook Acclimation Project and Nez Perce
    Tribal Hatchery.*

    1.  **Broodyear 2005 Fall Chinook**

        1.  **NPT Fall Chinook Acclimation Project -- Big Canyon
            Facility -** *The Big Canyon Acclimation facility is a
            portable acclimation setup designed and operated for
            acclimation and release of Snake River fall Chinook salmon
            that are reared at Lyons Ferry Hatchery. Big Canyon facility
            is operated by the Nez Perce Tribe as part of the Fall
            Chinook Acclimation Project (FCAP) funded by BPA. The
            facility has capacity to acclimate 150,000 yearlings and
            500,000 subyearlings. The facility is operated in
            conjunction with two other acclimation facilities on the
            Snake River in an effort to restore ESA listed Snake River
            fall Chinook salmon and achieve the LSRCP mitigation goal of
            18,300 adults to the project area.*

            1.  [Production status]{.underline} -- Approximately 160,000
                yearlings are being reared at Lyons Ferry Hatchery for
                transfer to the Big Canyon acclimation facility on March
                1-3, 2007. *Bruce McLeod* */ Mike Key*

            2.  [Projected release]{.underline} -- Target release will
                be 160,000 yearlings at 10 fpp on April 15. Fish are
                70,000 CWT and ad clipped, 80,000 CWT only, and 10,000
                unmarked (see M&E section below). (**Table 11**) *Bruce
                McLeod* */ Mike Key*

            3.  [Fish health]{.underline} - Yearling fish at Lyons Ferry
                SFH will be 1/17/07 for ELISA and viral assays for the
                import permit for this program. Monitoring samples for
                BKD will be taken weekly and a 60 fish sample will be
                collected and assayed prior to release from each site.
                *Kathy Clemens*

            4.  [M&E]{.underline} -

> Usually 100% of this group is CWT, however 10,000 of the 2007 release
> will be released un-marked due to a surplus of fish at all acclimation
> sites. Yearling release groups will be sampled for length at weight at
> time of release. We will sample 100 fish from each tank as they are
> being released. We sample 500 fish from each raceway at LFH for coded
> wire tag and adipose fin clip retention 30 days after tagging/marking
> is completed. We will PIT tag 5,000 yearlings to estimate survival,
> migration rate and timing through the FCRPS. All mortalities at Big
> Canyon will be scanned for PIT tags. Aerial redd counts and adult
> spawner carcass sampling in the Clearwater subbasin will be conducted
> by NPTH M&E personnel. Coded wire tags will provide SAR data. *Bill
> Arnsberg*

5.  [Communication]{.underline} - O&M and M&E quarterly and annual
    reports to BPA.

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2.  **Broodyear 2006 Fall Chinook**

    1.  **NPT -- Fall Chinook Acclimation Project -- Big Canyon
        Facility**

        1.  [Production status]{.underline} -- Approximately 520,000
            subyearlings are being reared at Lyons Ferry Hatchery for
            transfer to the Big Canyon acclimation facility on May
            01, 2007. *Bruce McLeod* */ Mike Key*

        2.  [Projected release]{.underline} -- Target release is 500,000
            subyearlings at 75-50 fpp on June 1, 2007. A group of
            100,000 fish are CWT / ad-clipped and 100,000 CWT only for
            evaluation -- the balance of fish are unmarked. (**Table
            11**) *Bruce McLeod* */ Mike Key*

        3.  [Fish health]{.underline} - Import permit sampling will be
            done in March/April. A 60 fish sample will be collected and
            assayed prior to release from each site. *Kathy Clemens*

> [Juvenile M&E]{.underline} -- Subyearling release groups will be
> sampled for length at weight at time of release. We will sample 100
> fish from each tank as they are being released. We sample 500 fish
> from each raceway at LFH for coded wire tag and adipose fin clip
> retention 30 days after tagging/marking is completed. We will PIT tag
> 2,500 subyearlings to estimate survival, migration rate and timing
> through the FCRPS. All mortalities at Big Canyon will be scanned for
> PIT tags. Aerial redd counts and adult spawner carcass sampling in the
> Clearwater subbasin will be
>
> conducted by NPTH M&E personnel. Coded wire tags will provide SAR
> data. *Bill Arnsberg*

4.  [Communication]{.underline} - O&M and M&E quarterly and annual
    reports to BPA.

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2.  **Nez Perce Tribal Hatchery** *-- Nez Perce Tribal Hatchery was
    constructed in 2001 and is authorized to produce 1.4 million
    subyearling fall Chinook juveniles. Target releases are 500,000 on
    station at Site 1705, 500,000 acclimated and released from North
    Lapwai Valley facility, 200,000 acclimated and released from Lukes
    Gulch facility (South Fork Clearwater) and 200,000 acclimated and
    released from Cedar Flats facility (Selway River).*

    1.  [Ladder Operation -]{.underline} Ladder operations commenced on
        21, August 2006, and continued through 5, December 2006. A total
        of 63 adults and 74 jacks (\<53 cm) were collected at NPTH. An
        additional 2 males and 2 jacks were collected at DNFH. In
        September, 405 adult fall Chinook and 239 jacks (\<53 cm) were
        transported from Lower Granite Dam to NPTH for spawning. *Butch
        Harty*

    2.  [Spawning]{.underline} -- Over the course of nine weeks, a total
        of 209 females were spawned, which yielded 852,632 green eggs.
        Average eye up was 88.16%. *Butch Harty*

    3.  [Production status]{.underline} - Total fall Chinook eyed eggs
        on hand at NPTH: 751,700. *Butch Harty*

    4.  [Projected release]{.underline} - Anticipated release: 730,000
        sub-yearlings.

    -   [Site 1705]{.underline}: A release of 500,000 sub-yearlings into
        the Clearwater River at 50 fish per pound (9.07 grams) is
        planned. The fish will be transferred directly into the ponds
        from the marking trailer. [Unmarked fish (200,000) destined for
        release into the Clearwater River will be held in production
        room tanks at Site 1705 until they attain a size of \~180 fpp
        (2.52 grams). The fish will then be transferred and divided
        equally into the two fall Chinook acclimation ponds at Site 1705
        for acclimation and release]{.mark}. The ponds will be set-up
        and operational at least 2 days prior to receiving fish. Prior
        to release, 3,000 fish will be tagged with a PIT tag.
        Mortalities will be picked daily and the fish feed 2 times per
        day. At the start of the schedule volitional [release, lengths
        and weights will be taken and recorded on a minimum of 500
        fish.]{.mark} Prior to release, a minimum 60 fish sample will be
        collected from each release group for a pre-release health
        inspection. Bacteriology, virology and parasitic assays will be
        performed. [Scheduled release date from Site 1705 is June
        15^th^.]{.mark}

    -   [North Lapwai Valley]{.underline}: A release of 180,000
        sub-yearlings into Lapwai Creek at 50 fish per pound (9.07
        grams) is planned. Lapwai Creek water temperatures will be
        monitored from late February through mid-April. In the event the
        water temperatures in Lapwai Creek exceed 55º F (12.8º C) in
        late March, the 180,000 sub-yearlings would most likely not be
        transferred to the North Lapwai Valley Acclimation facility but
        held at Site 1705 and released into the Clearwater River. The
        transfer of the fish will occur in mid to late April. The
        facility will be set-up and operational at least 3 days prior to
        transfer of fish. Prior to release, 3,000 fish will be tagged
        with a PIT tag. PIT tagging operations will take place prior to
        water temperatures attaining 17º C. L[engths and weights will be
        taken and recorded on a minimum of 500 fish prior to
        release.]{.mark} Mortalities will be picked daily and the fish
        feed 2 times per day. Scheduled release date for North Lapwai
        Valley AF is May 30^st^.

-   [Cedar Flats]{.underline}: A release of 25,000 sub-yearlings into
    the Selway River at 50 fish per pound (9.07 grams) is planned.
    Transfer of the fish will occur in late April to early May. The
    facility will be set-up and operational at least 5 days prior to
    transfer of fish. Prior to release, 4,900 fish will be tagged with a
    PIT tag. L[engths and weights will be taken and recorded on a
    minimum of 500 fish prior to release.]{.mark} Mortalities will be
    picked daily and the fish feed 2 times per day. Schedule release
    date from Cedar Flats AF is June 15^th^. However, an earlier release
    may occur to avoid unfavorable water temperatures.

-   [Lukes Gulch]{.underline}: A release of 25,000 sub-yearlings into
    the South Fork Clearwater River at 50 fish per pound (9.07 grams) is
    planned. Transfer of the fish will occur in late April to early May.
    The facility will be set-up and operational at least 5 days prior to
    transfer of fish. Prior to release, 4,900 fish will be tagged with a
    PIT tag. L[engths and weights will be taken and recorded on a
    minimum of 500 fish prior to release.]{.mark} Mortalities will be
    picked daily and the fish feed 2 times per day. Scheduled release
    date from Lukes Gulch AF is June 15^th^. However, an earlier release
    may occur to avoid unfavorable water temperatures. (**Table 11**)
    *Butch Harty*

    1.  [Fish health]{.underline} -- Kidney samples were assayed by
        ELISA on all spawned females; no eggs were culled due to ELISA
        OD's all below cut-off (.25). 150 ovarian fluid samples, 60
        tissues samples and 30 cranial samples were taken for assay.
        IHNV was found in 8 % of samples tested to date. A 60 fish
        sample will be collected and assayed prior to release. *Kathy
        Clemens*

    2.  [M&E]{.underline}

        -   Scan all fish for CWT. Initial tag retention and tagging
            mortality estimated. Estimate final CWT retention rates 14
            days or more after tagging.

        -   PIT survival studies- Pit tag 3,000 of each release group
            for survival estimates, growth rates, and migration timing.

        -   Redd surveys and carcass collection. Scales and genetic
            samples taken, hatchery/wild determination, scan for PIT
            tags and CWTs, along with all other biological information.

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-   Volunteers to NPTH will be scanned for PIT tags and CWTs and scales
    and genetics will be taken on a sub-sample of spawned fish and
    mortalities, along with all other biological information. *Bill
    Arnsberg / Jay Hesse*

    1.  [Communication]{.underline} - NPTH produces monthly production
        and pathology reports, and M&E quarterly and annual reports to
        BPA.

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    1.  [Dworshak NFH]{.underline}

        1.  [Transportation Study]{.underline} -- Fall Chinook salmon
            were temporarily reared at Dworshak NFH in 2005 and 2006 for
            the transportation study. However,

> there will be no fall Chinook reared at Dworshak in 2007, due to lack
> of fish available for research purposes. *Howard Burge*

1.  **Broodyear 2007 Fall Chinook**

    1.  **[Adult collection]{.underline} -** Snake River Fall Chinook
        adults will be collected at Lower Granite Dam (LWG) and
        transported to Site 1705, in accordance with the U.S. vs. Oregon
        Interim Management Agreement. Additionally, adult fall Chinook
        may enter the fish ladder and be trapped at Site 1705.

        1.  [Lower Granite Dam]{.underline} - Trapping and transport
            activities will occur under an ESA Section 10 Permit Number
            1530 and will be coordinated with Lyons Ferry Hatchery,
            Jerry Harmon and U.S. Army Corps of Engineers. Adult fall
            Chinook will be trapped at LWG commencing the last week of
            August for brood year 2007 or as soon as water temperatures
            allow. Trapping will continue throughout the run and is
            anticipated to end by late November or early December. Fall
            Chinook are collected in the trap as a sub-sample of the
            returning run. The sub-sample rate for 2007 has not yet been
            determined, but should range from 13-15% of the run over
            LWG. Currently, all adults trapped at LWG and retained for
            broodstock will receive a right operculum punch (ROP). Also,
            all females trapped will be injected with erythromycin and
            oxytetracycline during the sorting process. Washington
            Department of Fish and Wildlife (WDFW) Fish Management,
            Lyons Ferry Hatchery (LFH) and NPTH will develop a
            transportation schedule for adults trapped at LWG. The goal
            of NPTH is to receive 30% of the adults trapped at LWG
            (anticipated to be approximately 750 fish).

> [A portion of known LFH origin and unknown origin hatchery fall
> Chinook will be transported from LWG to Site 1705 for holding and
> spawning. Coded wire tagged adults, excess to broodstock needs, will
> be sacrificed for run-reconstruction purposes. Accurate
> run-reconstruction to LWG is based on a set sub-sampling protocol that
> requires that all hauled fish be accounted for (i.e., CWT's read and
> scales read on unmarked/untagged fish for expansion of fish not
> sampled at LWG). Adults, excess to broodstock needs and without a CWT,
> will be anesthetized and scale samples taken before they are released
> into the Clearwater River]{.mark}. *Butch Harty, Becky Johnson*

2.  [Trapping sites/Ladder operation]{.underline} - Commencing on August
    20, 2007, and continuing through December 4, 2007, the adult ladder
    and trap will be operated at Nez Perce Tribal Hatchery to collect
    fall Chinook adults for broodyear 2007. Adults that voluntarily
    return to NPTH will be injected with erythromycin prior to the first
    spawning. Adults will receive formalin treatments every-other day to
    control fungus and decrease pre-spawning mortality. *Butch Harty,
    Becky Johnson*

3.  [Spawning plans]{.underline} -- To meet the egg take goal of 1.978
    million, approximately 565 females will be spawned at NPTH. Spawning
    will begin October 30, 2007, and may continue through December
    4, 2007. At the completion of the third spawn (Nov. 13, 2007),
    un-spawned females may be injected with sGnRHa /Ovaplant-3. All
    out-of-Snake River Basin

> adults, identified as "strays" by CWT or other distinguishing marks,
> will be culled. WDFW may use scale pattern data to cull suspect
> "strays" and eggs. However, scale pattern data will not be used at
> NPTH in the culling of eggs. On all fish spawned the following data
> will be collected: Fork length, sex, fin clips, visual implant
> elastomer (VIE) tags (document side and color i.e. LR for left red),
> coded wire tag identification number and opercle punches and side
> V-notches, radio tag number if present and any other identifying marks
> or tags. All fish will be scanned for a PIT tag and scales will be
> taken on all unmarked/untagged fish with a sub-sample taken on CWT
> tagged fish. In addition, tissue samples will be taken on a random
> sub-sample of 100 spawned females and 100 spawned males for DNA
> analysis and genetic monitoring. Data entry, verification and
> finalization of all data collected will be coordinated with WDFW and
> completed by mid-January 2006. Finalized database files will be sent
> to NPT (Bill Arnsberg, WDFW (Debbie Milks) and Technical Advisory
> Committee (TAC) (Cindy Lefleur). [Coded wire tagged adults, excess to
> broodstock needs, will be sacrificed for run-reconstruction purposes.
> Accurate run-reconstruction to LWG is based on a set sub-sampling
> protocol that requires that all hauled fish be accounted for i.e.,
> CWT's read and scales read on unmarked/untagged fish for expansion of
> fish not sampled at LWG. Adults excess to broodstock needs and without
> a CWT, will be anesthetized and scale samples taken before they are
> released into the Clearwater River.]{.mark} Adults transported from
> Lower Granite Dam (LWG) and excess to broodstock needs will be
> sampled. All CWT tagged adults will be sacrificed for
> run-reconstruction purposes. All fish transported from LWG without a
> CWT and, not needed for broodstock, will be anesthetized and scales
> taken for run-re-construction purposes (wild/hatchery origin) prior to
> release into the Clearwater River. Every adult female will sampled
> individually for BKD with ELISA. Up to 150 ovarian fluid samples (3
> fish pools) will be sampled for viruses. In addition 60 tissues
> samples will be taken for bacteria assays, and sampled for *Myxobolus*
> cerebralis. Broodstock fish health samples will be taken by NPTH staff
> and delivered to Idaho Fish Health Center for analysis. *Butch Harty*

4.  [Egg Incubation]{.underline} -- Fertilized eggs will be water
    hardened for one hour in 100 parts per million (PPM) iodophore and
    placed in iso-incubation units. At between 550 and 620 temperature
    units (TU's) eyed eggs will be shocked; machine sorted the following
    day and transferred into Heath trays. Eggs will be incubated on
    processed (well and treated, chilled surface) water at a temperature
    of 52º F (11.1º C). Eggs from females with a BKD ELISA value of 0.2
    and above will be culled. All eggs should be hatched out by late
    January or early February. At swim-up, \~1,500 fish per pound (0.30
    grams), the fish will be transferred to production room tanks.
    *Butch Harty*

5.  [Egg transfers from Lyons Ferry]{.underline} - Egg transfers from
    Lyons Ferry Hatchery may occur for broodyear 2007 depending on the
    broodstock availability for NPTH and Lyons Ferry Hatchery. *Becky
    Johnson*

6.  [Adult M&E []{.mark}]{.underline}

    -   Redd surveys and carcass collection. Scales and genetic samples
        taken, hatchery/wild determination, scan for PIT tags and CWTs,
        along with all other biological information.

-   Volunteers to NPTH will be scanned for PIT tags and CWTs and scales
    and genetics will be taken on all spawned fish and mortalities,
    along with all other biological information. *Bill Arnsberg, Jay
    Hesse*

    1.  [Fish health]{.underline} -- Every adult female will be sampled
        individually for BKD with ELISA. Up to 150 ovarian fluid samples
        (3 pool) will be sampled for viruses. An additional 60 tissue
        samples will be taken for bacteria assays, and so samples for
        *Myxobolus cerebralis.* Brood fish health samples will be taken
        by NPT staff and delivered to Idaho Fish Health Center personnel
        for analysis. Fish with a high BKD titer will be culled. *Kathy
        Clemens*

    2.  [Communication]{.underline} - NPTH produces monthly production
        and pathology reports, and Fish Research produces quarterly and
        annual reports to BPA.

4.  **RAINBOW TROUT**

    1.  **USFWS Program**

        1.  **Dworshak Kids' Fishing Day**

            1.  [Production status - BY05]{.underline}: Dworshak is
                rearing Shasta strain rainbow trout from Ennis NFH for
                the June 15, 2007 Kids\' Fishing Day. On December

            2.  1, 2006, there were 16,529 rainbows at Dworshak.
                Approximately 3,800 of these trout will be transported
                to Kooskia NFH for their Open-House. The rainbow trout
                are currently being reared in two Burrow's ponds in
                System III. The Service hopes to achieve 15 inches in
                length (0.75 per lb.) by Open House. No fish health
                problems to date in this group of fish. *Thomas Trock*

            3.  [Excess outplanting]{.underline} - The plan is to
                continue what has been done in previous years, with
                excess fish going to the Nez Perce Tribe \~ 40%; Coeur
                D' Alene Tribe \~ 20%; and Idaho Dept. of Fish and Game
                \~ 40%. *Howard Burge*

    2.  **IDFG Programs --**

> *IDFG Nampa Hatchery rainbows experienced an Ich outbreak, so fish
> were treated seven days/week to help control the infection.
> Mortalities have subsided to single digits. Treatments are being
> curtailed and then will cease for 4 weeks. Necropsies will be
> performed to see if the infection is still present. If the treatments
> are successful the planned Clearwater basin releases are described
> below. If the treatments are unsuccessful IDFG will make adjustments
> to fish disposition. Efforts will be taken to ensure only fish from
> clean hatcheries are used for redistribution from hatcheries in
> sensitive areas.*

1.  **Dworshak Reservoir**

> Nampa Fish Hatchery plans on stocking 50,000 sterile triploid rainbows
> into Dworshak Reservoir in May- July. Since 1997 Hagerman NFH has
> raised rainbows for stocking into Southern Idaho reservoirs and IDFG
> reciprocates by stocking Dworshak Reservoir. There are no planned
> changes for the program this year. *Jerry McGehee* / *Howard Burge*

2.  **Clearwater Basin**

> IDFG stocks approximately 50,000 Kamloops rainbow trout and 160,000
> Spokane rainbow trout annually into the Clearwater River system. For
> 2006, the Kamloops rainbow are raised at Lyons Ferry and Nampa Fish
> Hatcheries (25,000 each) and stocked into the lower Clearwater River
> in October, after AD and RV-clipping.
>
> Spokane rainbow from Lyons Ferry Hatchery will be stocked into lowland
> lakes within the Clearwater drainage in April and May; these unmarked
> fish provide additional fishing opportunities. This program is funded
> by the Lower Snake River Compensation Plan and the Dingle-Johnson
> Program to compensate for dam related losses. *Robert Hand*
>
> The Clearwater Fish Hatchery regional rainbow program redistributes
> 100,000 Nampa reared trout. A total of 29 plant sites, requiring 110
> trips are stocked May to August. *Jerry McGehee*

5.  **PACIFIC LAMPREY**

    1.  **NPT Program**

        1.  **Nez Perce Tribal Hatchery -** In 2006, the Nez Perce Tribe
            initiated a Pacific Lamprey restoration initiative. On
            December 4, 2006, the NPT transported 178 adult lampreys
            that were collected in the dewatered ladder at John Day Dam
            to Nez Perce Tribal Hatchery/Site 1705. In an effort to
            control Furunculous, the lampreys were injected with
            oxytetracycline. Adults were then transferred into the M&E
            tanks located adjacent to the NATURES "S" channel release
            structure. To prevent escapement each tank is sealed with a
            plywood lid secured with clamps. The water flow source is
            from the Clearwater River, which is excess to NPTH
            production requirements. The M&E tanks outfall returns to
            the Clearwater River downstream of the fish ladder. The NPT
            Trout Ponds program is responsible for the care and
            maintenance of these fish. The adults will be held until the
            spring of 2007, when the NPT will release them in selected
            streams to spawn naturally. NPT is currently working on a
            release plan that will identify release locations and
            numbers. *Butch Harty*

6.  **CONTACTS**

Contacts, affiliation, phone number, and email addresses.

+----------------+-------------+--------------+-----------------------+
| # Name         | Agency      | Phone No.    | email                 |
+----------------+-------------+--------------+-----------------------+
| #              |             |              |                       |
+----------------+-------------+--------------+-----------------------+
| #              | NPT -       | 208-476-7296 | billa@nezperce.org    |
|  Bill Arnsberg | Research    |              |                       |
+----------------+-------------+--------------+-----------------------+
| #              | IDFG -      | 208-799-5010 | bbo                   |
| Brett Bowersox | Lewiston    |              | wersox@IDFG.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| # Howard Burge | FWS -- IFRO | 208-476-7242 | howard_burge@fws.gov  |
+----------------+-------------+--------------+-----------------------+
| #              | FWS -- IFHC | 208-476-9500 | kathy_clemens@fws.gov |
|  Kathy Clemens |             |              |                       |
+----------------+-------------+--------------+-----------------------+
|                |             |              |                       |
+----------------+-------------+--------------+-----------------------+
| Scott Everett  | NPT --NPTH  | 208-843-7320 | scotte@nezperce.org   |
|                |             | x-2442       |                       |
+----------------+-------------+--------------+-----------------------+
| Brad George    | IDFG -- CFH | 208-476-3331 | b                     |
|                |             |              | george@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Robert Hand    | IDFG -      | 208-799-5010 | rhand@idfg.idaho.gov  |
|                | Lewiston    |              |                       |
+----------------+-------------+--------------+-----------------------+
| Jerry Harmon   | NOAA -- LWG | 509-843-3058 | jerry.harmon@noaa.gov |
+----------------+-------------+--------------+-----------------------+
| Butch Harty    | NPT -- NPTH | 208-843-7384 | hartyhb@nezperce.org  |
+----------------+-------------+--------------+-----------------------+
| Chris          | IDFG --     | 208-465-8404 | ch                    |
| Harrington     | Research    |              | arring@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Jay Hesse      | NPT --      | 208-843-7145 | jayh@nezperce.org     |
|                | Research    | x-3040       |                       |
+----------------+-------------+--------------+-----------------------+
|                |             |              |                       |
+----------------+-------------+--------------+-----------------------+
| Dave Hurson    | COE --      | 509-527-7125 | dave.f.               |
|                | Walla Walla |              | hurson@usace.army.mil |
+----------------+-------------+--------------+-----------------------+
| # Ran          | IDFG -- CFH | 208-476-3331 | rhutze                |
| dy Hutzenbuler |             |              | nbiler@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| # Adam Izbicki | FWS -- KNFH | 208-926-4272 | adam_izbicki@fws.gov  |
+----------------+-------------+--------------+-----------------------+
| #              | NPT --      | 208-843-7320 | beckyj@nezperce.org   |
|  Becky Johnson | Production  | x-2433       |                       |
+----------------+-------------+--------------+-----------------------+
| Ed Larson      | NPT --      | 208-843-7320 | edl@nezperce.org      |
|                | Production  | x-2440       |                       |
+----------------+-------------+--------------+-----------------------+
| Mike Key       | NPT -- FCAP | 208-843-7320 | mikek@nezperce.org    |
|                |             | x-2486       |                       |
+----------------+-------------+--------------+-----------------------+
| Sharon Kiefer  | IDFG --     | 208-334-3791 | s                     |
|                | Manag.      |              | kiefer@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Joe Krakker    | FWS --      | 208-378-5321 | joe_krakker@fws.gov   |
|                | LSRCP       |              |                       |
+----------------+-------------+--------------+-----------------------+
| Scott Marshall | FWS --      | 208-378-5321 | s                     |
|                | LSRCP       |              | cott_marshall@fws.gov |
+----------------+-------------+--------------+-----------------------+
| Nancy          | NPT --      | 208-843-7320 | nancymac@nezperce.org |
| McAllaster     | Commission  | x- 2445      |                       |
+----------------+-------------+--------------+-----------------------+
| Jerry McGehee  | IDFG -- CFH | 208-476-3331 | jm                    |
|                |             |              | cgehee@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Bruce McLeod   | NPT -       | 208-843-7320 | brucem@nezperce.org   |
|                | Production  | x-2403       |                       |
+----------------+-------------+--------------+-----------------------+
| Doug Munson    | IDFG --     | 208-939-2413 | d                     |
|                | Fish Health |              | munson@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Jill Olson     | FWS --IFRO  | 208-476-7242 | jill_olson@fws.gov    |
+----------------+-------------+--------------+-----------------------+
| Aaron Penney   | NPT -- NPTH | 208-843-7384 | aaronp@nezperce.org   |
+----------------+-------------+--------------+-----------------------+
| Mike Delarm    | NOAA --     | 503-230-5412 | mike.delarm@noaa.gov  |
|                | Portland    |              |                       |
+----------------+-------------+--------------+-----------------------+
| Greg Parker    | COE --      | 208-476-1251 | greg.a.               |
|                | Dworshak    |              | parker@usace.army.mil |
+----------------+-------------+--------------+-----------------------+
| Larry Peltz    | FWS -- DFC  | 208-476-4591 | larry_peltz@fws.gov   |
+----------------+-------------+--------------+-----------------------+
| Tom Rogers     | IDFG --     | 208-334-3791 | t                     |
|                | Production  |              | rogers@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Ralph Roseberg | FWS -- IFRO | 208-476-7242 | r                     |
|                |             |              | alph_roseberg@fws.gov |
+----------------+-------------+--------------+-----------------------+
| Bob Semple     | FWS -- DNFH | 208-476-4591 | bob_semple@fws.gov    |
+----------------+-------------+--------------+-----------------------+
| Ed Schriever   | IDFG --     | 208-799-5010 | edsch                 |
|                | Lewiston    |              | riever@idfg.idaho.gov |
+----------------+-------------+--------------+-----------------------+
| Sam Sharr      | IDFG --     | 208-334-3791 | ssharr@idfg.idaho.gov |
|                | Manag.      |              |                       |
+----------------+-------------+--------------+-----------------------+
| Sherman        | NPT -       | 208-476-4044 | shermans@nezperce.org |
| Sprague        | Research    |              |                       |
+----------------+-------------+--------------+-----------------------+
| Todd Sween     | NPT --      | 208-843-7320 | tods@nezperce.org     |
|                | Production  | x-2389       |                       |
+----------------+-------------+--------------+-----------------------+
| Thomas Trock   | FWS -- DNFH | 208-476-4591 | thomas_trock@fws.gov  |
+----------------+-------------+--------------+-----------------------+
| Jason Vogel    | NPT --      | 208-843-7145 | jasonv@nezperce.org   |
|                | LSRCP       | x-3602       |                       |
+----------------+-------------+--------------+-----------------------+