**LOWER SNAKE RIVER FISH AND WILDLIFE COMPENSATION PLAN**

**GRANDE RONDE AND IMNAHA BASINS ANNUAL OPERATION PLAN**

**FOR THE PERIOD OF**

**FEBRUARY 1, 2007 -- JANUARY 31**, **2008**

**PREPARED BY:**

**OREGON DEPARTMENT OF FISH AND WILDLIFE**

**CONFEDERATED TRIBES OF THE UMATILLA INDIAN RESERVATION**

**NEZ PERCE TRIBE**

**FOR**

**LOWER SNAKE RIVER COMPENSATION PLAN**

**U.S. FWS ADMINISTRATION**

**and**

**BONNEVILLE POWER ADMINSTRATION**

**Final**

**February 2, 2007**

######### 

In Attendance December 19, 2006 pre-AOP: CTUIR (Crump, McLean, Weldert),
ODFW (Deal, Flesher, Gribble, Monzyk, Onjukka, Patterson, Smith), NPT
(Cleary, Harbeck, Michaels, Sealy, Zollman)

In Attendance January 9, 2007 AOP 2007: CTUIR (McLean, Zimmerman), ODFW
(Flesher, Hoffnagle, Knox, Lund, Onjukka, Patterson), NMFS (), NPT
(Cleary, Harbeck, Johnson, Sealy, Vogel, Zollman), LSRCP-FWS ()

**TABLE OF CONTENTS**

Table of
Contents................................................................................ii

List of
Tables....................................................................................ii

List of
Appendices.............................................................................iii

**Steelhead [(Oncorhynchus
mykiss)]{.underline}**........................................
...........1

I. Summer Steelhead 2006 Brood Year (Grande Ronde & Imnaha basins)
......1

II\. Summer Steelhead 2007 Brood Year Wallowa Stock
............................3

III\. Summer Steelhead 2008 Brood Year Wallowa Stock-fall
collection........... 5

IV\. Summer Steelhead 2007 Brood Year Little Sheep
Stock..................... ...6

V.  Summer Steelhead-Monitoring; Catherine Creek/Grande Ronde

> River/Lookingglass Creek in
> 2007.................................................. 8

VI\. Summer Steelhead 2007 NPT- Imnaha
Tributaries................................8

**Chinook ([O. tshawytscha]{.underline})**
.................................\...\...\...............\...\................9

VII\. Grande Ronde Basin-2005 Brood Year-Spring/Summer Chinook

-stocks Grande Ronde, Catherine, & Lostine\.......\.............
............9

VIII\. Grande Ronde Basin --2006 Brood Year- Spring/Summer Chinook

--Catherine, Lookingglass, Lostine, & U. Grande Ronde
.................11

IX\. Grande Ronde Basin-Conventional 2007 Brood Year- Spring/Summer
Chinook - Catherine, Lookingglass, Lostine & U. Grande
Ronde........................12

X. Grande Ronde Basin-Captive 2007 Brood Year Spring/Summer Chinook

stocks Grande Ronde, Catherine, & Lostine ...........
..................17

XI\. Imnaha Basin -- 2005 Brood Year- Spring/Summer Chinook...\...
............17

XII\. Imnaha Basin -- 2006 Brood Year- Spring/Summer Chinook....
...........18

XIII.Imnaha Basin -- 2007 Brood Year- Spring/Summer Chinook....
..........18

**List of Tables**

Table 1. Irrigon Hatchery StS transfer schedule
2007\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...
22

Table 2. Juvenile spring chinook salmon and summer steelhead

sampling schedule at LSRCP facilities,
2007.........\...\...\...\...\...\...\.... 23

Table 3. Genetics Monitoring Sampling
2007\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\...\.....
23

Table 4. PIT-tagging schedule for 2007 brood summer steelhead

at Irrigon
Hatchery.........\...\...\...\...\...\.......................................
24

Table 5. Summer Steelhead run projections to LSRCP Facilities in
2007.....\...\...\...\..... 24

Table 6. Summary of projected number of tagged releases for the 2006
brood summer

steelhead and 2005 brood spring chinook
salmon\...\...\...\...\...\...\...\... 25

Table 7. PIT-tagging schedule for 2006BY spring chinook

salmon at Lookingglass Fish Hatchery in
2007\...\...\...\...\...\...\...\...\...\...\..... 25

Table 8. Projected spring Chinook salmon returns to Catherine Creek in
2007......\...\... 26

Table 9. Projected spring Chinook salmon returns to U. Grande Ronde in
2007.........................................................................\....
26

Table 10. Projected spring Chinook salmon returns to Lostine River in
2007......\...\... 26

Table 11. Projected spring Chinook salmon returns to the Imnaha River in
2007...\..... 27

Table 12. Projected spring Chinook salmon returns to Lookingglass Fish
Hatchery

in
2007\...\...\...\...\..........................................................\.....
27

**List of Appendices**

Appendix A. Proposed juvenile salmonid releases in the

Grande Ronde and Imnaha basins in
2007\...\...\...\...\...\...\...\...\...\...\..... .....29

Appendix B. Steelhead Fish Health Monitoring Plan & Disease
Treatments...... ..30

Appendix C. Disinfections and Sanitation Guidelines for Hatchery
Facilities........ .31

Appendix D. Juvenile Chinook Fish Health Monitoring Plan & Disease
Treatments 34

Appendix E. Adult Chinook Fish Health Monitoring Plan & Disease
Treatments... .35

Appendix F. Ponding Plan for 2007 at Lookingglass
Hatchery..................... ....36

Appendix G. Snake River Germplasm Repository Cryopreserved Semen Request
Form..............................................................................
37

Appendix H. Table Summarizing Adult Collection Fish Health Injection
Protocols for Grande Ronde and Imnaha
Chinook..........................................38

Appendix I. Attachment for Imnaha and Grande Ronde Broodstock Antibiotic
Injection........................................................................
40

Appendix J. Catherine Creek, Lostine, and Upper Grande Ronde fish
culture production metrics summarized for Grande Ronde sub-basin Chinook
stocks............................................................... 41

Appendix K. Preliminary CTUIR data from Catherine Creek releases between
1998 and 2007 summarized by ODFW................................ 42

Appendix L. Preliminary CTUIR data from U. Grande Ronde releases between
2000 and 2007 summarized by
ODFW......................................... 43

Appendix M. Interim data from Imnaha River releases between 1984 and
2007...............................................................................
44

######## Steelhead [(*Oncorhynchus mykiss*)]{.underline}

######## [I. Summer Steelhead - 2006 Brood Year (Grande Ronde & Imnaha basins)]{.underline}

This is third year for releasing smolts from fall collected brood stock
evaluation. Fall component is 100,000 smolts marked 100% with an AdRV
clip and CWT. A portion (\~3,600 smolts) are implanted with a PIT tag.
The release is paired with spring-collected brood with similar numbers,
AdLV clip, CWT, and PIT tags. The identifying external difference is the
ventral fin clip.

**[A. Allocations]{.underline}** --The estimated number of smolts from
Irrigon is 1,065,000 fish weighing 253,000 pounds. A total of 800,000
are Wallowa stock at 4fpp. Wallowa fish will be acclimated at the
Wallowa and Big Canyon facilities and released both with an early group
and late group component. A total of 265,000 are Little Sheep stock at
5fpp. The Little Sheep stock will be acclimated in the Little Sheep
facility and also direct released in Big Sheep. Smolt transfers and
releases are summarized in Table 1 and Appendix A.

## [B. Liberations]{.underline}

**1. Schedule**

a.  **Wallowa Acclimation:** Approximately 480,000 smolts will be
    released form the Wallowa acclimation site, 360,000 in the early
    group and 120,000 in the late group.

+--------------+-----------+-----------+------------------------------+---+
| #####        |           |           |                              |   |
| ### Early Gr |           |           |                              |   |
| oup: Approxi |           |           |                              |   |
| mately 360,0 |           |           |                              |   |
| 00 smolts wi |           |           |                              |   |
| ll be releas |           |           |                              |   |
| ed after 5 t |           |           |                              |   |
| o 6 weeks of |           |           |                              |   |
|  acclimation |           |           |                              |   |
+--------------+-----------+-----------+------------------------------+---+
| Location     | Transfer  | ####      | ######## Comments            |   |
|              | in date   | #### Rele |                              |   |
|              |           | ase dates |                              |   |
+--------------+-----------+-----------+------------------------------+---+
| Lower        | February  | #         | The screens in the lower     |   |
| Acclimation  | 14-20     | ####### A | sections will be pulled on   |   |
| Pond         |           | pril 8-Su | April 8 allowing fish to     |   |
|              |           |           | leave for 24 hours. On April |   |
|              |           | 24        | 9, the remaining fish will   |   |
|              |           | hr-v      | be forced out of the lower   |   |
|              |           | olitional | section                      |   |
+--------------+-----------+-----------+------------------------------+---+
|              |           | April 9-M |                              |   |
|              |           |           |                              |   |
|              |           | Forced    |                              |   |
+--------------+-----------+-----------+------------------------------+---+
| Upper        | February  | April 9-M | The screens in the upper     |   |
| Acclimation  | 20-23     |           | sections will be pulled on   |   |
| Pond         |           | 24        | Monday allowing fish to      |   |
|              |           | hr-v      | leave for 24 hours. On April |   |
|              |           | olitional | 10, the remaining fish will  |   |
|              |           |           | be forced out of the lower   |   |
|              |           |           | section                      |   |
+--------------+-----------+-----------+------------------------------+---+
|              |           | April     |                              |   |
|              |           | 10-T      |                              |   |
|              |           |           |                              |   |
|              |           | Forced    |                              |   |
+--------------+-----------+-----------+------------------------------+---+
| Note:        |           |           |                              |   |
| A            |           |           |                              |   |
| pproximately |           |           |                              |   |
| 150,000      |           |           |                              |   |
| smolts       |           |           |                              |   |
| released     |           |           |                              |   |
| will be used |           |           |                              |   |
| for fall     |           |           |                              |   |
| collected    |           |           |                              |   |
| brood        |           |           |                              |   |
| evaluations. |           |           |                              |   |
+--------------+-----------+-----------+------------------------------+---+

+--------------+-----------+-----------+------------------------------+
| Late Group:  |           |           |                              |
| A            |           |           |                              |
| pproximately |           |           |                              |
| 120,000      |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 2 to 3 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| Lower        | April     | April     | The screens in the lower     |
| Acclimation  | 16-18     | 28-Sa     | section will be pulled on    |
| Pond         |           |           | April 28 allowing fish to    |
|              |           | 12 day    | leave for 12 days. On May    |
|              |           | v         | 10, the remaining fish will  |
|              |           | olitional | be forced out of the upper   |
|              |           |           | and lower sections           |
+--------------+-----------+-----------+------------------------------+
|              |           | May 10-Th |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| Note:        |           |           |                              |
| A            |           |           |                              |
| pproximately |           |           |                              |
| 50,000       |           |           |                              |
| smolts       |           |           |                              |
| released     |           |           |                              |
| will be used |           |           |                              |
| for fall     |           |           |                              |
| collected    |           |           |                              |
| brood        |           |           |                              |
| evaluations  |           |           |                              |
+--------------+-----------+-----------+------------------------------+

> **b. Big Canyon Acclimation:** Approximately 320,000 smolts will be
> released from the Big Canyon acclimation site, 160,000 in the early
> group and 160,000 in the late group.

+--------------+-----------+-----------+------------------------------+
| Early Group: |           |           |                              |
| A            |           |           |                              |
| pproximately |           |           |                              |
| 160,000      |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 5 to 6 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| Lower        | March 5-6 | #         | The screens in the lower     |
| Acclimation  |           | ####### A | sections will be pulled on   |
| Pond         |           | pril 11-W | April 11 allowing fish to    |
|              |           |           | leave for 24 hours. On April |
|              |           | 24        | 12, the remaining fish will  |
|              |           | hr-v      | be forced out of the lower   |
|              |           | olitional | section                      |
+--------------+-----------+-----------+------------------------------+
|              |           | ##        |                              |
|              |           | ###### Ap |                              |
|              |           | ril 12-Th |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| Upper        | March 6-7 | April     | The screens in the upper     |
| Acclimation  |           | 12-Th     | sections will be pulled on   |
| Pond         |           |           | April 12 allowing fish to    |
|              |           | 24 hr-    | leave for 24 hours. On April |
|              |           | v         | 13, the remaining fish will  |
|              |           | olitional | be forced out of the lower   |
|              |           |           | section                      |
+--------------+-----------+-----------+------------------------------+
|              |           | April     |                              |
|              |           | 13-F      |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+

+--------------+-----------+-----------+------------------------------+
| Late Group:  |           |           |                              |
| A            |           |           |                              |
| pproximately |           |           |                              |
| 160,000      |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 2 to 3 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| Lower        | April     | May 1 -T  | The screens in the lower     |
| Acclimation  | 18-19     |           | section will be pulled on    |
| Pond         |           | 10 day    | May 1 allowing fish to leave |
|              |           | v         | for 10 days. On May 11, the  |
|              |           | olitional | remaining fish will be       |
|              |           |           | forced out.                  |
+--------------+-----------+-----------+------------------------------+
|              |           | May 11 -  |                              |
|              |           | F         |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| Upper        | April     | May 2 -W  | The screens in the upper     |
| Acclimation  | 19-20     |           | section will be pulled on    |
| Pond         |           | 10 day    | May 2 allowing fish to leave |
|              |           | v         | for 9 days. On May 11, the   |
|              |           | olitional | remaining fish will be       |
|              |           |           | forced out.                  |
+--------------+-----------+-----------+------------------------------+
|              |           | May 11 -  |                              |
|              |           | F         |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| Note: Prior  |           |           |                              |
| to May 12    |           |           |                              |
| ODFW Fish    |           |           |                              |
| Research     |           |           |                              |
| will sample  |           |           |                              |
| smolts in    |           |           |                              |
| the          |           |           |                              |
| acclimation  |           |           |                              |
| pond. If     |           |           |                              |
| \>70% of the |           |           |                              |
| sample       |           |           |                              |
| contains     |           |           |                              |
| males, fish  |           |           |                              |
| will be      |           |           |                              |
| enumerated   |           |           |                              |
| and up to    |           |           |                              |
| 2,000        |           |           |                              |
| released in  |           |           |                              |
| Wallowa      |           |           |                              |
| Wildlife     |           |           |                              |
| pond and 500 |           |           |                              |
| in Victor.   |           |           |                              |
+--------------+-----------+-----------+------------------------------+

c.  **Little Sheep Acclimation**: Approximately 265,000 smolts will be
    released in the Little Sheep and Big Sheep tributaries, 165,000
    acclimated in Little Sheep and 100,000 direct stream released in Big
    Sheep

+--------------+-----------+-----------+------------------------------+
| Acclimation. |           |           |                              |
| A            |           |           |                              |
| pproximately |           |           |                              |
| 165,000      |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 5 to 9 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| Acclimation  | March 7-9 | April     | Screens will be pulled on    |
| Pond         |           | 10-T      | April 10 allowing fish to    |
|              |           |           | leave for 20 days. On May 1, |
|              |           | 20 day    | the remaining fish will be   |
|              |           | v         | forced out.                  |
|              |           | olitional |                              |
+--------------+-----------+-----------+------------------------------+
|              |           | May 1 - T |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| Note: Prior  |           |           |                              |
| to May 1     |           |           |                              |
| ODFW Fish    |           |           |                              |
| Research     |           |           |                              |
| will sample  |           |           |                              |
| smolts in    |           |           |                              |
| the          |           |           |                              |
| acclimation  |           |           |                              |
| pond. If     |           |           |                              |
| \>70% of the |           |           |                              |
| sample       |           |           |                              |
| contains     |           |           |                              |
| *maturing    |           |           |                              |
| males*,      |           |           |                              |
| n            |           |           |                              |
| on-migrating |           |           |                              |
| fish will be |           |           |                              |
| enumerated   |           |           |                              |
| and released |           |           |                              |
| in Kinney    |           |           |                              |
| Lake. Kinney |           |           |                              |
| Lake out     |           |           |                              |
| flow is      |           |           |                              |
| diverted     |           |           |                              |
| into         |           |           |                              |
| irrigation   |           |           |                              |
| canal that   |           |           |                              |
| discharges   |           |           |                              |
| into the     |           |           |                              |
| Grande       |           |           |                              |
| Ronde.       |           |           |                              |
+--------------+-----------+-----------+------------------------------+

.

> **d. Big Sheep direct release**: Approximately 100,000 smolts will be
> released directly into Big Sheep April 9-11. NPT will check with
> Thompson's to make sure the gate is open to access Big Sheep.

**[C. Monitoring and Evaluation]{.underline}**

**1. Summary of marked steelhead released in 2007**

**a. Wallowa**

-   100,000 Ad, RV, CWT

-   100,000 Ad, LV, CWT

```{=html}
<!-- -->
```
-   280,000 Ad only

**b. Big Canyon marks include**:

-   50,000 Ad, LV, CWT

-   270,000 Ad only

c.  **Little Sheep**

-   140,000 Ad only

-   25,000 Ad, LV, CWT

**d. Big Sheep** (direct release) marks include:

-   100,000 no mark

**2. Fish Research---**Fish Research staffs will coordinate efforts with
the hatchery staffs for pre-release sampling and other marking efforts
(Tables 2 and 3).

**3. Irrigon Hatchery --**Irrigon staff will measure fish lengths for
the Big Sheep release.

**[D. Fish Health]{.underline}**---Fish Health staff will coordinate
efforts with Fish Research and hatchery staffs to conduct pre-release
health examine (Appendix B). Standard disinfection and sanitation
guidelines will apply (Appendix C).

**[E. Satellite Operation]{.underline}**---Wallowa staff will set-up Big
Canyon acclimation facility ponds in late-February. Big Canyon adult
trap will be deployed in late-January, and adult traps for Wallowa and
Little Sheep will be installed in February, weather permitting.

**[F. Key Contacts]{.underline}**

**1. ODFW** (Davis) will notify the following Van Dyke (ODFW) of
steelhead releases

**2. ODFW (**Becker) will notify juvenile trapping personnel -- NPT-
(Michaels (426-3198; ext. 4), Vogel (208-816-1405) of any change in the
scheduled Little Sheep or Big Sheep direct stream smolt releases.

**[II. Summer Steelhead -2007 Brood Year - Wallowa Stock]{.underline}**

*The LSRCP mitigation goal is 9,200 adults.*

**[A. Smolt Goal]{.underline}** -- Produce 800,000 smolts at 4.0fpp for
release in 2008. Target production includes:

-   700,000 production

-   100,000 fall collected brood evaluation

Note: Production from fall collected brood stock above CWT evaluation
needs will be AdRV clipped as part of standard production group from
Wallowa Acclimation site.

**[B. Egg Take Goal]{.underline}** -- Collect 1,112,472 green eggs to
produce 990,100-eyed eggs (89% survival green to eyed eggs). Transfer
990,100-eyed eggs to Irrigon Hatchery to produce 800,000 smolts (80.8%
survival eyed eggs to smolt).

**[C. 2007 Adult Collection]{.underline}**

**1. Predicted Run** (Table 4)

-   Marked --3,020,

-   Unmarked --87

-   Total -- 3,107

**a. Wallowa Hatchery** --

-   Marked --1,932 (585 -- 3,279 95% CI)

    -   1,260 1:1-salt; 652 1:2-salts; 17 2:1-salt; 3 2:2-salt.

**b. Big Canyon Satellite** --

-   Marked --1,088 (58 -- 2,119 95% CI)

    -   691 1:1-salt; 391 1:2-salts; 6 2:1 salt.

-   Unmarked -- 87 (46 -- 129 95% CI)

    -   29 2:1-salt; 21 2:2-salts, 23 3:1 salt; 14 others

-   Total -- 1,175

# 

# D. Trap Operations

**1. Wallowa Trap Operation** -- Wallowa trap will be installed when
winter conditions allow typically in late-February. Collections will
continue until no fish are caught for 10 consecutive days.

**a. Trap/sorting Frequency** - Work trap Wednesdays with hatchery staff
as needed.

> **b. Disposition of Trapped Fish** - The estimated surplus of Wallowa
> stock (Wallowa and Big Canyon combined) is 2,326 adults. The majority
> of surplus fish will be distributed to food banks. ODFW Grande Ronde
> Fish District has requested stocking 50 fish in Roulet pond and 40
> fish in Ladd pond; and ODFW Wallowa district has requested 100 fish in
> Marr Pond and 70 fish in Wallowa wildlife pond. Stocking should occur
> by April 11^th^. Stocked fish will be identified by a missing adipose
> fin and 2-left opercle punched (2-LOP). Fish not out planted or given
> to Food Banks will be taken to the county landfill. Fish spawned will
> be taken to the county landfill.
>
> *1*) *Unmarked***---**Unmarked fish will be transported to the Fish
> Hatchery Lane Bridge and released. Samples include genetic (from
> opercle punch), sex, length.
>
> *2) Residual Steelhead --* Count and sample all residuals weekly, take
> snouts from all AdLV's, and euthanize all fish marked Ad only. After
> smolts have been released from acclimation pond, discontinue residual
> sampling.
>
> *3) Bull Trout --* Record date, number trapped, and estimated fork
> length (minimizing handling effects and avoid anesthetics). Send
> summary to Smith (ODFW) and Krakker (USFWS).

**2. Big Canyon Trap Operations**---No broodstock will be required from
Big Canyon. The Big Canyon trap will be installed when winter conditions
allow which is typically in early-February. Collections will continue
until no fish are caught for 10 consecutive days.

> **a. Period of Trap Operation** *-* From initial start-up through
> April 9, the ladder will be operated from 5 pm Monday through Friday
> morning. After sorting on Fridays, the ladder will remain closed
> through Monday 5 pm to keep hatchery fish more available to the
> angler. After April 9, the ladder will remain open throughout the
> trapping operation.

**b. Trap/sorting/recycle Frequency *-*** Work trap on Friday's.

**c. Disposition of Trapped Fish**

> 1\) *Unmarked\--*Pass all fish above the weir in Deer Creek. All
> released fish will be measured and marked with a 1-LOP.
>
> *2) Marked --* No marked fish will be passed. All adults marked AdLV
> or AdRV will not be releases and sampled for CWT recovery. Surplus
> hatchery fish will be outplanted, recycled or transferred to Wallowa
> Hatchery for disposition.
>
> *3) Re-cycle fish*\--Starting in late February and continuing through
> 6 April, approximately 50 fish will be re-cycled in the fishery every
> Friday. Fish will be uniquely marked with OP and outplanted at the
> Minam boat ramp (70%) and above Deer Creek confluence (30%)*.*
> Recorded data should include fish checked in creel surveys, release
> location, OP punch, number of weeks to return to Big Canyon, number
> fish unaccounted, number that returned to Wallowa Hatchery (stray).
> Re-captures will be processed to food banks or landfill.
>
> *4) Bull Trout --* Record date, number trapped, and estimated fork
> length (minimizing handling effects and avoid anesthetics). Send
> summary to Smith (ODFW) and Krakker (USFWS).
>
> *5) Residual Steelhead **-*** Count weekly until first smolt release.
> Sample all AdLV's and take snouts and euthanize all Ad only. Efforts
> will be made to prevent residual steelhead from escapement when
> working the adult trap.
>
> **d. Disposition of Fallback Fish** *-* Staff will collect and sample
> all fish that fallback on the Deer Creek weir to determine; passed to
> un-passed ratio, h/w ratio, and number spawned out. Collect otoliths
> or head from moribund or dead unmarked steelhead and pass live fish
> downstream.

# E. Hatchery Broodstock/Collection Guidelines

**1. Wallowa Hatchery-** All Wallowa steelhead are held and spawned at
Wallowa Hatchery.

a.  **Fall collected---**A total of 76 adults were collected in
    October 2006. In late February or early March, the surviving fall
    collected broodstock will be enumerated, sex determined, and
    externally marked with a Tyvek tag and LOP punched. A total of \~75
    fish may survive to January 1, 2007.

b.  **Winter/spring collection---**Approximately 359 additional
    steelhead should be spawned for broodstock needs. Production will
    consist of Ad only or AdLV's. Swim-in AdRV will not be utilized for
    the production group.

**c. Broodstock Needs** --A total of 434 adults should be spawned to
meet production goals.

-   Males -- 217

-   Females -- spawn 217.

Pre-spawning mortality of spring-collected adults is estimated at 1.3%
for females and 3.0% for males. Pre-spawning mortality of fall-collected
adults is estimated at 3.5%. The five-year average fecundity is 5,120
eggs/female.

**2. Wallowa Hatchery Spawning Guidelines**

**a. Expected 1st Spawn *--*** Wednesday, March 14. Thirty to
thirty-five pairs are expected to spawn from fall-collected broodstock.
The number below will be reduced proportionately for those specific
spawn days.

**b. Spawning Dates** *--* Wednesday.

-   March 14 -- 19 females (3 stacks)

-   March 21 -- 33 females

-   March 28 \-- 39 females

-   April 4 \-- 39 females

-   April 11 \-- 39 females

-   April 18 \-- 31 females

-   April 25 -- 17 females

If 9 females not ripe on March 14, production will be comprised from
only six egg takes. The total of 217 females will be spawned.

**c. Spawning Strategies** - 1:1 ratio and incubate 1 female per tray.
Segregate the eggs collected from fall returning broodstock. Males from
fall-collected brood may be used twice in the spawning protocols.

**[F. Incubation Strategies-]{.underline}Wallowa/Irrigon** Green eggs
will be incubated at Wallowa Hatchery. Embryos will be transferred to
Irrigon Hatchery as eyed eggs and will represent seven egg takes. The
first egg take will be delayed until March 14.

**[G. Rearing Program]{.underline}-Irrigon**

Eggs will be hatched and reared at Irrigon Hatchery.

**a. Programmed for Release** - The target transfer size is 4.5fpp for
an April and May releases.

> **b. Grade** -- Progeny from fall-collected adults will not be graded.
> Production releases will be graded. Fish will be fed at differing
> rates for targeted transfer dates.
>
> **c. Excess** -- No excess is expected, however, if survival is
> greater than expected, eggs can be culled, smolts produced, or
> released in Kinney Lake.
>
> **d. Progeny** from fall-collected broodstock will be segregated.
> Progeny not required for evaluation will be mixed after RV marking in
> the general production and used in Wallowa releases.

**[H. Fish Health]{.underline}**

**a. Broodstock** monitoring plan (Appendix B).

**b. Disinfection** and Sanitation Guidelines (Appendix C).

## [I. Monitoring & Evaluation]{.underline} 

> **a. Proposed marking includes:** Progeny from fall-collected
> broodstock will be reared in two release groups. The first release
> will consist of 75% of the production and second 25% of the
> production. This will complete the third year of marking for this fall
> v winter/spring brood evaluation.

**1. Wallowa** (480,000 total, 360,000 first acclimation and 120,000
second)

-   100,000 Ad, LV, CWT

    -   75,000 AdLV first acclimation

    -   25,000 AdLV second acclimation

-   100,000 Ad, RV, CWT (fall returning broodstock)

    -   75,000 AdRV first acclimation

    -   25,000 AdRV second acclimation

    -   280,000 Ad only

> **2. Big Canyon** (320,000 total)

-   50,000 Ad, LV, CWT

-   270,000 Ad only

**b.** Tagged groups are summarized in Table 5.

**c. PIT-tagging** in each release group (Table 6).

**d. Re-cycle---**ODFW Fish Research **(**Flesher) will provide a short
summary of fish re-cycled in the fishery.

### III. Summer Steelhead -2008 Brood Year - Wallowa Stock---Fall Collection 

*No fall brood collections are expected in 2008.*

### IV. Summer Steelhead - 2007 Brood Year -- Little Sheep Stock

Co-managers have agreed to manage production to meet the LSRCP
mitigation goal of 2,000 adults. The goal consists of fish returning to
Little Sheep, Big Sheep, and compensation area harvest.

**[A. Smolt Goal]{.underline}** ---- Produce 265,000 smolts at 5.0fpp
for release in 2008.

Production and releases include:

-   165,000 Little Sheep Cr. (acclimated) smolts (at least 5% wild
    parents)

-   100,000 Big Sheep Cr. (direct stream) smolts

**[B. Egg Take Goal]{.underline}** -- A total 367,595 green eggs will be
taken to produce 327,160 eyed eggs (89%) and 265,000 smolts (81% eyed
eggs to smolts).

**[C. Adult Collection]{.underline}**

**1. Predicted Run** -- (Table 5).

-   Marked -- 1,637 (549 to 2,724 95% CI);

    -   1,229 1:1-salt; 388 1:2-salts; 18 2:1-salts; 2 3:1

-   Unmarked -- 90 (0 to 180 95% CI)

    -   48 2:1-salt and 19 2:2-salts; 17 3:1-salts; 6 3:2-salts and
        4:1-salts.

**[D. Weir Management and Trap Operations]{.underline}**

**1. Little Sheep Trap Operation** -- Little Sheep trap will be
installed when winter conditions allow typically in late-February.
Collections will continue until no fish are caught for 10 consecutive
days.

**a. Trap/sorting Frequency** - Work trap Mondays and Thursdays.

**b. Disposition of Trapped Fish**

> *1) Wild* --1 of 4 males and 1 of 7 females will be taken for brood
> (total wild fish collected is estimated at 8 males and 8 females).
> Wild composition in hatchery brood is estimated at 10.1 %. The other
> wild adults collected will be 1-LOP and placed above the weir. Number
> wild fish released above the weir is estimated at 74 fish with a wild
> composition of 33% for natural spawning.
>
> *2) Hatchery* -- For males, 1 of 10 will be collected for brood, 1 of
> 10 marked with 1-LOP and placed above the weir, and 8 of 10 marked
> with 2-LOP and out planted to Big Sheep. For females, 1 of 12 will be
> collected for broodstock, 1 of 12 marked with 1-LOP and released above
> the weir, and 10 of 12 marked with 2-LOP out planted to Big Sheep. The
> run size will be reviewed around April 1 and adjustment can be made
> for broodstock collections. NPT will provide two people two days per
> week for Big Sheep adult outplants. Number hatchery fish released
> above the weir is estimated at 150 fish with a hatchery composition of
> 67% for natural spawning.
>
> *3) Bull Trout* -- Record date, number trapped, and estimated fork
> length (minimizing handling effects and avoid anesthetics). Send
> summary to Smith and Zakel (ODFW) and Krakker (USFWS).
>
> *4) Residual Steelhead* -- Count and sample all residuals weekly until
> first smolt release, take snouts from all AdLV's and euthanize all Ad
> only.
>
> *5) Genetics tissue samples*. All wild and hatchery fish passed above
> the weir will be tissue sampled for genetic analysis by ODFW (for
> NMFS).
>
> *6) Big Sheep out plant*s. Approximately 1,345 adults will be
> transferred from Little Sheep to Big Sheep. Outplants will be
> identified with 2-LOP. Outplants can be discontinued after May 10 if
> \< 2 fish per week are collected. Fish captured after May 10 may be
> released above the weir.
>
> *7) Fallback or Recaptured Fish* -All recaptured Big Sheep (2-LOP) and
> Little Sheep (1-LOP) fish will be counted and released in their target
> stream at recapture and subsequent recaptures.

*8) Carcass Disposal*-Spawned fish and mortalities will be placed in the
local landfill.

*9) Strays* -- All unidentified marked fish will be sacrificed.

*10) Scales*---Samples will be collected from all wild adults

**[E. Collection Guidelines]{.underline}**---Adults returning to Little
Sheep trap will have a variety marks. Summary of marks include:

#### Mark Disposition

> Ad Out plant or pass above the weir\
> AdLV+CWT Out plant, pass above weir, or spawn\
> No Mark+CWT (blank wire) Out plant or pass above weir\
> No Mark wild Spawn or pass above weir
>
> No Mark hatchery Out plant or pass above weir\
> AdRV (out of basin) Kill not spawn (recover CWT)
>
> \* Check all no marks for CWT
>
> \* For Passed or Outplanted \-- record clip, sex, location, genetic
> sample for passed fish, fork length
>
> \* For KNS \-- record date and Clip, then save for sampling

\* The dorsal fin will be inspected to help identify no mark hatchery
fish.

**1. Broodstock Needs**-

-   Males --80 collected (77 spawned)

-   Females --78 collected (77 spawned)

Broodstock numbers were determined based on a 5-year average of
pre-spawning mortality (1.0% females; and 3.0% males) and mean fecundity
of 4,700.

> **a. Wild** broodstock-- 8 males and 8 females needed for brood.
> Approximately 74 wild fish will be released above Little Sheep weir.
>
> **b. Hatchery** broodstock-- 72 males and 70 females are needed for
> brood. Approximately 150 adults will be released above the Little
> Sheep weir. The balance of hatchery origin adults may be outplanted in
> Big Sheep Creek

.

**[F. Spawning Guidelines]{.underline}**

**1. Little Sheep Satellite**

**a. First Spawn** - March 20.

**b. Expected Spawning Frequency** -- Weekly on Tuesdays.

> **2. Spawning Strategies** -- A 3 x 3 matrixes with hatchery and wild
> fish will be utilized. A matrix will include at least one natural
> fish, when ever possible. When eggs have been fertilized, the embryos
> will be combined into groups of three females. These groups will be
> tracked.

-   Note: Live spawn all wild males retained for broodstock and release
    (genetic sample).

**[G. Incubation Strategies]{.underline}**

Green eggs will be incubated at Wallowa Hatchery. Embryos will be
transferred to Irrigon Hatchery as eyed eggs and will represent all egg
takes.

**[H. Rearing Program]{.underline}**

Embryos will be hatched and fish reared at Irrigon Hatchery.

**1. Programmed for Release** - 265,000 smolts

-   100,000 Big Sheep

-   165,000 Little Sheep

Target size at transfer is 5.0fpp. Single acclimation is expected with
early May release.

**2. Grade** -- No Little Sheep stock will be graded.

> **3. Excess production** - Fish in excess of program needs will be
> reared to smolts and incorporated with the Little Sheep Creek release.

**[I. Fish Health]{.underline} -** Monitoring Plans

**1. Disinfection** and Sanitation Guidelines (Appendix C).

**2. Broodstock** monitoring plan (Appendix B)

**[J. Monitoring & Evaluation]{.underline}**

**1. Proposed marking includes:**

**a. Little Sheep**:

-   25,000 Ad, LV, CWT

-   140,000 Ad only

**b. Big Sheep** (direct release):

-   100,000 no mark

**2. PIT tagging** in each release group (Table 6).

> **3. Pedigree genetic analysis---**Little Sheep adults are being
> evaluated on their origin (hatchery or wild) using genetic samples.
> All fish released above the weir and used for brood stock are sampled.

**[K. Key contacts]{.underline}**

> **1. ODFW** (Flesher) will provide ODFW (Smith, Davis, Patterson), NPT
> (B. Johnson, Hesse, Vogel) and CTUIR (Zimmerman, Boe, McLean,
> Schwartz) with weekly summary on collected and passed StS adults at
> Little Sheep.

**[V. Summer Steelhead Monitoring: Catherine Creek/Grande Ronde
River/Lookingglass Creek-2006]{.underline}**

*Goal-to monitor natural escapement and hatchery strays into natural
production areas and collect basic life history information for
management planning---No fish production goals.*

**[A. Monitoring and Evaluations]{.underline}**

**1. Adult Enumeration/Weir Collections**

> **a. Weir location-**Catherine Creek (CC), Grande Ronde River (UGRR),
> and Lookingglass Creek (LGCR). CC and UGRR weirs installed, operated
> and maintained by CTUIR. LGCR weir installed and operated by ODFW
>
> **b. Period of Trap Operation** -- Environmental conditions
> permitting, March through August 1, typically no steelhead captured
> after mid-June. Lookingglass trap will be operated into early
> September to collect chinook.

**2. Disposition of fish at weir-**

> **a**. **Steelhead** -- Unmarked adults will be sampled (enumerated,
> weighed, fork length, sex, marks, condition) opercle punched and
> passed above the weir. Marked steelhead will be sacrificed and AdLV
> marked fish will be sampled for CWT recovery.
>
> **b**. **Bull trout** -- Record date, number trapped, and estimated
> fork length (minimizing handling effects and avoid anesthetics). Send
> summary to Smith, Zakel, and Jacobs (ODFW) and Krakker (USFWS).

**c**. **Genetic** -- Collect tissue samples from all wild steelhead for
future genetic analysis.

> **d.** **Adult mortality** -- Retain Lookingglass Creek mortality
> only. Sample immediately or freeze for sampling. Other weir locations,
> remove otolith (ODFW) and return carcass to river. Collaborate with
> Fish Health when working dead fish.
>
> **e. Fallback** fish will be collected and released below the weir.
> Recaptured fish will be released above weir.

**3. Juvenile Sampling**

**a.** Operate rotary trap(s) on Lookingglass Creek **-** Trap
year-round, collect data, PIT tag, release..

**[B. Key Contacts]{.underline}**

**1**. CTUIR (Boe). Distribute bull trout and steelhead data collected
to ODFW District offices.

**[VI. Summer Steelhead - Imnaha Tributaries (Lightning and Cow
Creeks)]{.underline}**

Goal-to quantify adult escapement (NPT)---No Production goals.

**[A. Monitoring and Evaluations]{.underline}**

**1. Adult Escapement -** Enumeration Weir using angled upstream and
downstream portable picket weirs

**a. Weir location-**Lightning Creek and Cow Creek.

**2. Trap Operations**

**a.** *Wild/Hatchery* -- No fish retained. Trap, collect data, and
release only.

**b.** *Wild Kelts* -- No collection.

**c.** *Period of Trap Operation* -- February through June, or until 10
days after last capture.

**d.** *Trapping Strategies*-check trap twice daily.

**e.** *Disposition of fish at weir*-

> **1)** Steelhead -- Steelhead in the upstream movement box will dipped
> out with cotton dip net and place into a moist canvas sling/measuring
> box. Data including fin clips, sex, spawning condition (pre/post), and
> fork length will be recorded. Scales will be collected from just
> behind the dorsal fin and above the lateral line using a blunt knife
> and forceps. A paper hole punch will be used to collect fin tissue
> from the dorsal fins for DNA genetic characterization before release
> upstream of the trap. A double right opercular punch will be given
> using a paper hole punch and a Tyvek disk tag will be applied to the
> left operculum. Non-target species will be measured (sub-sample
> 25/day/species) and released. Steelhead and non-target species will be
> release into a pool/slack water above the weir.
>
> Steelhead captured in the downstream movement box will be examined for
> opercular punches and Tyvek disk tags. Marked fish will be checked for
> spawning condition (pre or post-spawn) and released downstream.
> Downstream moving non-marked steelhead and non-target species will be
> handled with the same procedures as upstream moving fish with the
> exception of a downstream release.
>
> **2)** Bull trout -- Record date, number trapped, and estimated fork
> length (minimizing handling effects and avoid anesthetics). Report
> take to US Fish and Wildlife Service Under Section 6 (4d limitation)
> Bull Trout Permit #TE001598-1 with copy of data to ODFW (Smith) and
> LSRCP (Krakker).

**3)** Genetic - Collect tissue samples from all wild and hatchery fish
for future genetic analysis.

> **f.** ***Disposition of adult mortality*** -- Natural mortalities
> will be sampled for biological information and their otoliths
> collected.

**[C. Key Contacts]{.underline}**

**1**. NPT (Vogel, Hesse)

####### CHINOOK *[(O. tshawytscha)]{.underline}*

*Fish production will prioritize 12 raceways for Grande Ronde tributary
production and 6 raceways for Imnaha production at Lookingglass
Hatchery. Priorities include:*

-   *Lostine; 4 raceways; 3 raceways conventional and 1 raceway captive
    broodstock*

-   *Upper Grande Ronde;* *4 raceways.*

-   *Catherine Creek; 2 raceways*

-   *Lookingglass Creek; 2 raceways*

-   *Imnaha; 6 raceways*

**[VII. Grande Ronde Basin -2005 Brood Year Spring/Summer Chinook--
Catherine Creek, Lookingglass Creek, U. Grande Ronde & Lostine
River]{.underline}**

*Smolts target size is 25fpp by October 31 with a expected release size
of 20fpp in April.*

**[A. Allocation---]{.underline}**The estimated number of smolts for the
Grande Ronde Subbasin 2007 release is 441,300 fish weighing 20,000
pounds. Breakdown by tributary is as follows:

-   Catherine Creek (CC)- 71,300

    -   CBS-21,500

    -   Conv-49,800

-   Lostine River (LR)- 230,400

    -   CBS-24,700

    -   Conv-205,700

-   U. Grande Ronde (UGR)- 139,600

    -   CBS-20,600

    -   Conv-119,000

-   Lookingglass (LGCR)-0

# 

# B. Liberations

**1. Schedule**---All facilities will be set-up and operational at least
2 days prior to scheduled delivery of smolts. Weather permitting; the
Lostine is scheduled for delivery of fish on March 5, Catherine Creek on
March 12, and on Upper Grande March 13. Acclimation facility operator
will notify Bob Becker, Bob Lund, or Scott Patterson if their facility
is not operational on scheduled dates (Appendix A). Release number will
be determined by last physical inventory minus mortality. Facility
operators will report final numbers to the ODFW LGH or Shari Beals.

**a. Lostine Acclimation schedule**

+---------------+----------+-----------+------------------------------+
| Early Group:  |          |           |                              |
| Approximately |          |           |                              |
| 105,300       |          |           |                              |
| smolts will   |          |           |                              |
| be released   |          |           |                              |
| after 1 to 3  |          |           |                              |
| weeks of      |          |           |                              |
| acclimation.  |          |           |                              |
+---------------+----------+-----------+------------------------------+
| Location      | Transfer | ####      | ######## Comments            |
|               | in date  | #### Rele |                              |
|               |          | ase dates |                              |
+---------------+----------+-----------+------------------------------+
| LGH ½ R10 to  | March    | March     | The screens will be pulled   |
| pond A        | 5 - 6    | 16-F      | on March 16 allowing fish to |
|               |          |           | leave for 10 days. On March  |
|               |          | 10 day    | 26, the remaining fish will  |
|               |          | v         | be forced out                |
|               |          | olitional |                              |
+---------------+----------+-----------+------------------------------+
| LGH ½ R10 to  |          |           |                              |
| pond B        |          |           |                              |
+---------------+----------+-----------+------------------------------+
| LGH ½ R8 to   |          | March     |                              |
| pond C        |          | 26-M      |                              |
|               |          |           |                              |
|               |          | Forced    |                              |
+---------------+----------+-----------+------------------------------+
| LGH ½ R8 to   |          |           |                              |
| pond D        |          |           |                              |
+---------------+----------+-----------+------------------------------+
|               |          |           |                              |
+---------------+----------+-----------+------------------------------+

+---------------+-----------+-----------+-----------------------------+
| Late Group:   |           |           |                             |
| Approximately |           |           |                             |
| 125,100       |           |           |                             |
| smolts will   |           |           |                             |
| be released   |           |           |                             |
| after 1 to    |           |           |                             |
| 3.5 weeks of  |           |           |                             |
| acclimation.  |           |           |                             |
+---------------+-----------+-----------+-----------------------------+
| Location      | Transfer  | ####      | ######## Comments           |
|               | in date   | #### Rele |                             |
|               |           | ase dates |                             |
+---------------+-----------+-----------+-----------------------------+
| LGH 2/3 R9 to | March     | April     | The screens will be pulled  |
| pond A        | 27-28     | 7-Sa      | on April 7 allowing fish to |
|               |           |           | leave for 10 days. On April |
|               |           | 0 day     | 17, the remaining fish will |
|               |           | v         | be forced out               |
|               |           | olitional |                             |
+---------------+-----------+-----------+-----------------------------+
| LGH 1/3 R9    |           |           |                             |
| and 1/3 R7 to |           |           |                             |
| pond B        |           |           |                             |
+---------------+-----------+-----------+-----------------------------+
| LGH R11 to    |           | April     |                             |
| pond C        |           | 17-T      |                             |
|               |           |           |                             |
|               |           | Forced    |                             |
+---------------+-----------+-----------+-----------------------------+
| LGH 2/3 R7 to |           |           |                             |
| pond D        |           |           |                             |
+---------------+-----------+-----------+-----------------------------+
| Note: R11     |           |           |                             |
| captive brood |           |           |                             |
| progeny       |           |           |                             |
+---------------+-----------+-----------+-----------------------------+

b\. Catherine Creek

+--------------+-----------+-----------+------------------------------+
| A            |           |           |                              |
| pproximately |           |           |                              |
| 71,300       |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 3 to 4 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| LGH ½ R1 to  | March 12  | March     | The screens will be pulled   |
| pond A       |           | 26-M      | on March 26 allowing fish to |
|              |           |           | leave for 16 days. On April  |
|              |           | 16 day    | 11, the remaining fish will  |
|              |           | v         | be forced out                |
|              |           | olitional |                              |
+--------------+-----------+-----------+------------------------------+
| LGH ½ R1 to  |           |           |                              |
| pond B       |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| LGH R2 to    |           | April     |                              |
| pond C       |           | 11-W      |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| MT pond D    |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| R1 and R3    |           |           |                              |
| are captive  |           |           |                              |
| production,  |           |           |                              |
| R2           |           |           |                              |
| conventional |           |           |                              |
| production   |           |           |                              |
+--------------+-----------+-----------+------------------------------+

c\. Upper Grande Ronde

+--------------+-----------+-----------+------------------------------+
| A            |           |           |                              |
| pproximately |           |           |                              |
| 139,600      |           |           |                              |
| smolts will  |           |           |                              |
| be released  |           |           |                              |
| after 3 to 4 |           |           |                              |
| weeks of     |           |           |                              |
| acclimation. |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| Location     | Transfer  | ####      | ######## Comments            |
|              | in date   | #### Rele |                              |
|              |           | ase dates |                              |
+--------------+-----------+-----------+------------------------------+
| LGH R3 to    | March     | March     | The screens will be pulled   |
| pond A       | 13-14     | 26-M      | on March 26 allowing fish to |
|              |           |           | leave for 16 days. On April  |
|              |           | 16 day    | 11, the remaining fish will  |
|              |           | v         | be forced out                |
|              |           | olitional |                              |
+--------------+-----------+-----------+------------------------------+
| LGH R4 to    |           |           |                              |
| pond B       |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| LGH R5 to    |           | April     |                              |
| pond B       |           | 11-W      |                              |
|              |           |           |                              |
|              |           | Forced    |                              |
+--------------+-----------+-----------+------------------------------+
| LGH R6 to    |           |           |                              |
| pond B       |           |           |                              |
+--------------+-----------+-----------+------------------------------+
| R6 is        |           |           |                              |
| captive      |           |           |                              |
| brood        |           |           |                              |
| production:  |           |           |                              |
| CTUIR        |           |           |                              |
| requested a  |           |           |                              |
| contingency  |           |           |                              |
| for captive  |           |           |                              |
| brood        |           |           |                              |
| releases if  |           |           |                              |
| smolts are   |           |           |                              |
| larger than  |           |           |                              |
| 22fpp at     |           |           |                              |
| transfer.    |           |           |                              |
+--------------+-----------+-----------+------------------------------+

d\. Lookingglass Creek

+-------+---+------------+-----------------------------------------------+
| No    |   |            |                                               |
| fish  |   |            |                                               |
| were  |   |            |                                               |
| rel   |   |            |                                               |
| eased |   |            |                                               |
| from  |   |            |                                               |
| Lo    |   |            |                                               |
| oking |   |            |                                               |
| glass |   |            |                                               |
| Creek |   |            |                                               |
+-------+---+------------+-----------------------------------------------+
| Loc   |   | ##         | ######## Comments                             |
| ation |   | ###### Rel |                                               |
|       |   | ease dates |                                               |
+-------+---+------------+-----------------------------------------------+
| NA    |   |            |                                               |
+-------+---+------------+-----------------------------------------------+

**Notes:**

**Contingency---**Fish may be released earlier than schedule if
conditions warrant. Downstream rotary traps operators should be notified
immediately and co-manager within 24 hours: Erick VanDyke (541)
962-3783; Jeff Yankee (541) 426-6057; Pat Keniry (541) 962-3026.

**All acclimation mortalities** will be scanned for PIT tags. Tags or
code numbers will be provided to NPT (Cleary) and ODFW (Jonasson).
Mortalities will be offered to fish health for examination

**[D. Monitoring and Evaluation]{.underline}**---A variety on M&E
efforts are ongoing (Table 2, 3 and 5).

> **1**. Genetic tissue collection for monitoring and potentially
> pedigree analysis.
>
> **2.** Pre-liberation weight and length
>
> **3.** Downstream migration.
>
> **4**. PIT survival studies
>
> **5.** Tag retention and fin clip quality.
>
> **6.** Captive vs. Conventional production

7.  Hatchery vs. Natural

# 8. Marked Groups

> **a.** AD/CWT *-* 100% in CC, LR, and uGR captive brood and 100% in CC
> and LR conventional brood
>
> **b.** CWT only (no Ad clip)- 100% uGR conventional smolts.
>
> **c.** PIT tag *by stock--* CC 21k, LGCR 5k, uGR 1k, LR Captive brood
> 8k, LR Conventional brood 6.5k.
>
> **d.** Visual Implant Elastomer (VIE) --

-   CC-Blue-left eye in captive

-   LR --Red- left eye in captive

**[E. Fish Health]{.underline}**\-- Fish Health staff will coordinate
efforts with Fish Research and hatchery staffs to conduct pre-release
health examine (Appendix D). Standard disinfection and sanitation
guidelines will apply (Appendix C).

# F. Key contacts

**1. Hatcheries:** CTUIR (McLean), ODFW (Lund, Deal), NPT (Zollman).

**2. Fish Research:** CTUIR (Boe) ODFW (Hoffnagle, Monzyk), and NPT
(Hesse, Harbeck, Vogel).

**[VIII. Grande Ronde Basin -2006 Brood Year Spring/Summer Chinook
--Catherine, Lookingglass, Lostine & U. Grande Ronde]{.underline}**

*Smolts target size is 25fpp by October 31 and 20fpp at release.*

**[A. Allocation]{.underline} --**Scheduled for transfer target size of
*22fpp* in mid-March 2008.

**1. Anticipated Grande Ronde basin production** at Lookingglass
hatchery is 636,000 smolts for release in 2008.

**a. Estimated Captive brood** numbers are:

-   Catherine Creek 0

-   Lostine River 14,000

-   U. Grande Ronde 0 (outplanted \~1,400 eyed eggs in Meadow Creek
    11-2006)

-   Lookingglass Creek 48,000

b.  **Estimated Conventional brood** numbers are:

-   Catherine Creek 120,000

-   Lostine River 198,000

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-   U. Grande Ronde 256,000

**[B. Final Rearing]{.underline}---**LGH fry will be transferred outside
in April or early May (Appendix F).

Approximately 100,000 Lostine River fry will be transferred from Oxbow
to Lookingglass Hatchery in April at 250fpp.

**[C. Marking Program]{.underline}**\--

1.  **AD/CWT** is scheduled for June through July 2007. Catherine Creek,
    Lostine River conventional, and the captive brood Lookingglass Creek
    (CC-stock) production will be marked. The Lostine River captive
    brood will be CWT for M&E purposes. Marking of the Upper Grande
    Ronde was not resolved during in US v Oregon on January 24, and is
    still under discussion.

> **Catherine Creek**

-   60,000 Ad CWT

-   60,000 Ad only

> **Lostine River**

-   198,000 Ad CWT

-   14,000 CWT only (M&E mark)

> **Lookingglass Creek**

-   48,000 Ad CWT

> *Note: During marking, equipment will be disinfected between stocks.
> Within a stock, operations will start with low titer group and
> progress to higher titer groups. Raceways with abnormal mortality
> rates will be marked last by stock. Additional efforts will be made
> for equipment disinfections.*

**2. PIT tagging** is scheduled for October 2007 (Table 7). Numbers by
stock include:

-   Catherine Creek---21,000

-   U. Grande Ronde---2,000

-   Lostine (captive)---1,500

-   Lostine (conventional)---5,000

> **4. No Visual Implant Elastomer (VIE) is** scheduled in 2007 for
> BY06.

### 

### D. Fish Health

Only one medicated feed treatment is planned for conventional 2006
brood. In lieu of an early treatment, a proactive diet (immune enhancer)
will be applied. Captive brood fry will receive a medicated treatment,
if feed is available.

**1. Disinfection** and Sanitation Guidelines (Appendix C).

**2. Juvenile and disease outbreaks** (Appendix D).

**[E. Key contacts]{.underline}**

> **1. Fish Marking** (Lund, Mallette, Onjukka, Harbeck, Vogel, Monzyk
> and Jonasson)

**[IX. Grande Ronde Basin-Conventional-2007 Brood Year - Spring/Summer
Chinook Catherine, Lookingglass, Lostine, and Upper Grande
Ronde]{.underline}**

*The LSRCP production goal is 900,000 smolts to produced 5,800 adults.
However, co-managers will target 880,000 smolts for release in 2008.*

**[A. Smolt Production -]{.underline}**

**1.** Production targets include:

-   Catherine Creek: 130,000 smolts

-   Lookingglass Creek: 250,000 smolts

-   Lostine: 250,000 for Lostine River

    -   187,500 from conventional brood

    -   62,500 from captive brood

-   Upper Grande Ronde: 250,000 smolts

**[B. Anticipated Egg Needs]{.underline}** -- A total of 1,047,600 green
eggs should be collected to produce 880,000 conventional smolts based on
84% green eggs to smolts survival. Captive brood component can be used
to supplement production numbers.

# 

# C. 2007 Adult Collection 

**1. Predicted Runs** (Tables 8, 9, 10,11) female contribution estimated
at 50% of adults.

**a. Catherine Creek** **--**

> [ODFW-]{.underline}**248 adults (31 jacks)**

-   Natural-182 adults (plus 14jacks)

-   Hatchery-66 adults (plus 17 jacks)

##### CTUIR-**213 adults (6 jacks)**

-   Natural-150 adults (plus 4 jacks)

-   Captive-21 adults (plus 1 jacks)

-   Conventional-47 (plus 1 jacks)

**b. Lostine River**

> [ODFW]{.underline}**--665 Adults (212 jacks)**

-   Natural-506 adults (plus 47 jacks)

-   Hatchery-159 adults (plus 165 jacks)

> [NPT]{.underline}**--603 Adults (43 jacks)**

-   Natural- 400 adults (plus 23 jacks)

-   Captive- 59adults (plus 10 jacks)

-   Conventional-145 adults (plus 10 jacks)

**c. Upper Grand Ronde** --

> [ODFW-]{.underline}**130 adults (20 jacks)**

-   Natural-68 adults (plus 17 jacks)

-   Hatchery-62 adults (plus 3 jacks)

> [CTUIR-]{.underline}**77 adults (29 jacks)**

-   Natural-56 adults (plus 29 jack)

-   Captive-6 adults (plus 0 jacks)

-   Conventional-15 adults (plus 0 jacks)

**d. Lookingglass Creek 475 adults (30 jacks)**

> [CTUIR.]{.underline}

-   Natural-40 adults (plus 2 jacks)

-   Hatchery CC-435 adults (plus 28 jacks)

**2. Broodstock Needs** (Appendix J)

> **a. CC---**A target of 42 pairs should be collected to produce
> 130,000 smolts. The estimate is based on a female survival of 95%,
> fecundity of 3,824, and green eggs to smolt survival of 86%.
>
> **b. LG CR**---An estimated number of 80 pairs should be collected to
> produce 250,000 smolts. This is based on performance history of the CC
> stock.
>
> **c. LR---**A target of 70 a pairs should be collected to produce
> 250,000 smolts. If captive brood production is utilized, only 54 pairs
> should be collected (51 spawned). These estimates are based on female
> survival of 95%, fecundity of 4,370, and 85% green egg to smolt
> survival.
>
> **d. UGR**--- A target of 83 a pairs should be collected to produce
> 250,000 smolts. This is based on a female survival of 92%, fecundity
> of 4,062, and 81% survival from green egg to smolt.

**[D. Trap Operation]{.underline}**

> **1. CC and UGR Trap Operation** **(CTUIR)--** Trapping will begin in
> March 2007 to monitor steelhead abundance. Overnight staffing will
> occur after April 16 and will continue, if river conditions allow,
> through July 31.
>
> **2. LR Trap Operation (NPT)**\--Trapping on Lostine River will begin
> in May and continue through October 1.
>
> **3. Lookingglass (ODFW)---** The intake trap at Lookingglass Hatchery
> will be operated from mid-May (environmental conditions allow) through
> mid-September. Known returns of CC smolts released into Lookingglass,
> and unmarked jacks, will be used for outplants or broodstock. Unmarked
> adults will be given to the Tribes for C/S purposes.

**4. General Guidelines** --

> **a.** Trapping facilities will be checked daily.
>
> **b.** Water temperature data will be collected. When water
> temperature exceeds 65^o^F (18.3^o^C) fish will not be handled. Picket
> will be pulled and fish will be allowed to pass. It is expected that
> as water temperatures increase facility operates will adjust their
> schedule to best coincide their work with the coolest water
> temperatures. Water temperatures can be monitored with Onset
> temperature loggers.
>
> **c.** Surveys will be conducted by walking the stream bank for a mile
> section below each weir. Surveys frequency ranges from daily to weekly
> depending on water temperatures and fish activity. Information is used
> to determine if salmon are accumulating below the weirs. Surveys may
> include snorkeling.
>
> **d.** Attempts will be made to haul captured adults on a daily basis.
> However, adults in CC and UGR will be worked on M, W, F schedule, but
> will be worked more often during the peak of the run, if necessary.
> Fish may be held up to 72 hours.

**5. Weir Management Guidelines**

> **a. CC\--** At the projected run level of 213 to 248, the adult
> sliding scale for broodstock collection in Catherine Creek
> supplementation programs calls for collection of \<40% of wild and
> hatchery-origin fish. Hatchery-origin adults released above the weir
> is not a decision factor. Ten percent of the males above the weir may
> be age-3 hatchery males.

**1) Catherine Creek**

-   Wild fish---keep 2 of 5 for brood (40%), pass 3 of 5

-   Conventional---keep 2 of 5 for brood (40%), pass 3 of 5

-   Captive---pass 100% of captive adults

> **b. U. Grande Ronde**
>
> **1) UGR \--** Grande Ronde conventional program calls for collection
> of 50% of natural fish and up to 100% conventional returns. Pass 100%
> of captives.
>
> **c. Lostine\--** At the projected run level of 603 to 665 adults, the
> adult sliding scale for broodstock collection in Lostine River
> supplementation programs calls for collection of \<20% of wild fish.
> Hatchery-origin adults released above the weir should be ≤ 50%, and
> the hatchery brood stock should contain ≥30% wild origin fish. Ten
> percent of the males above the weir may be age-3 hatchery males. Pass
> or out plant all Captive Brood F~1~ returning adults.

-   Wild fish---keep 1 of 5 for brood (20%), pass 4 of 5

-   Conventional---keep 2 of 5 for brood (20%), pass 3 of 5

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-   Captive---pass or outplant 100% of captive adults

> Surplus adults are not expected, but maybe out planted in Bear Creek
> or Wallowa River.

**d. Lookingglass Creek---**Work trap as needed

> **1)** Unmarked chinook, considered progeny of Rapid River origin
> stock, will be given to the Tribes for C/S.
>
> **2)** Up to 150 ad clipped adults will be held at Lookingglass
> Hatchery. Outplants of adults can occur when the run is assessed in
> June. Weir management plan is under development.
>
> **3)** Surplus hatchery jacks can be euthanized.
>
> **4)** All Chinook passed upstream of the intake trap will have tissue
> collected for future genetic analysis (pedigree)

**Notes:**

**General comments---Less than** 5% marked fish from other streams or
basins will be passed upstream. If an unmarked PIT fish is detected, it
will be passed upstream or used for brood stock after verification of
origin. Surplus captive brood jacks from CC or UGR may be sacrificed for
CWT recovery.

**6. Disposition of Trapped Fish** \--Adults considered fish age-4 (two
ocean) and age-5 (three ocean). NPT plans to collect scales from passed
adults and jacks to help delineate between age-three, age-four and
age-five fish estimates using length frequencies.

> **a. Bull Trout** -- Enumerate and estimate length (minimize
> handling). Data and reports sent to ODFW (ODFW District and Regional
> offices), and LSRCP (Krakker).
>
> **b. Returning Adults from Captive Brood (F~1~)** -- Pass or out
> plant. Data include fish length, genetic (tissue), and sex prior to
> release above the weir. Excess jacks may be sacrificed for CWT
> recovery
>
> **c. Unmarked** **Chinook can** be anesthetized with Aqui-S or MS 222
> prior to handling. A data sheet should be provided to Lookingglass
> Hatchery for all transferred fish (AAT). Each fish trapped will be
> measured to the nearest mm fork length, sex determined, and a tissue
> sampled (opercle or caudal punches) for genetic analysis. Fish passed
> above the weir will be allowed to fully recover in sheltered flow
> before being released. Fish placed above the weir will be opercle
> punched (UGR=1ROP, CC=1ROP, LR=Unique opercle punch bi-weekly) for
> population estimates. *The opercle tissue is generally saved for
> genetic analysis*. LR fish taken to the Lookingglass will receive
> three opercle punches (3-ROP) and Tyvek tag. Catherine Creek fish
> taken to Lookingglass will be PIT tagged. Wild fish from Upper Grande
> Ronde transferred to Lookingglass will have an orange Tyvek tag and
> hatchery fish a white Tyvek tag. Hatchery fish trapped on Lookingglass
> Creek, identified as CC, LR, UGR, will either be released back to the
> stream of origin or held for with their respective brood, and marked
> with 2ROP. Adult may be outplanted after July 25 or spawned for
> production. If outplanted, Lookingglass returning fish will be opercle
> punched and tissue saved for genetic analysis.
>
> **d. Carcasses weirs** *--* Trapping mortalities will be processed as
> kept fish and transported to Fish health, fresh if possible, for
> pathological examination. Fish dead for less than 24 hrs keep on ice.
> Fish dead more than 24 hours freeze. Other pre-spawning mortalities
> discovered during weir-effect surveys will also be sent to Fish
> Health. Data will be sent to ODFW Fish Research (Monzyk). Following
> examination, the carcasses may be disposed of by Tribal distribution,
> habitat or landfill.

**Note: Tumors-** Fish will be inspected for tumors along the gum line.
If a tumor is suspected, fish with will be held for consultation.

**7. Broodstock Transportation** **Procedures** -- CTUIR will provide
transportation of adult fish from CC and uGR and NPT will provide
transportation from the Lostine. ODFW Regional Transport coordinator
will provide back-up transportation.

**a. Attempt to haul broodstock adults daily.** Adults will not be held
more than 72 hours.

> **b. Driver is responsible to complete a transfer data sheet** to the
> Lookingglass Hatchery staff upon arrival for data entry in the HMIS
> system.
>
> **c. Thermal shock will be minimized during transport**. Hauling will
> normally occur in the morning to take advantage of cooler stream
> temperatures. Temperature differences between transport container and
> facility water will not exceed 10^o^F or 5.6^o^C. Tempering may be
> necessary to reduce temperature difference.
>
> **d. Fish Handling-** Fish will be netted from the transport tank and
> placed in holding tanks at Lookingglass Hatchery. Lookingglass
> Hatchery personnel will record all observations on data sheets and
> report to Fish Health at the end of the season.

8.  **Adult holding**

    a.  **Catherine Creek --** All fish will be held in one adult
        holding raceway.

    b.  **Lookingglass Creek-**All fish will be held in one adult
        holding raceway.

    c.  **Lostine River-**All fish will be held in circular tank in
        endemic building (number TBA).

    d.  **Upper Grande Ronde---**All adults will be held in two circular
        tanks in the endemic building (numbers TBA). After first sort,
        males and females will be segregated.

**[E. Spawning Guidelines]{.underline}** (for each stock)

**1. Anesthetic** MS222 or Aqui-S.

**2. Sorting** -- Final sexing and inoculation week should occur the
week of August 1. Lostine and Lookingglass fish will be given IP
injections. Upper Grande Ronde and Catherine Creek fish will be given
dorsal sinus (DS) injections. No inoculation of jacks.

**3. Expected First Spawn** -- The week of August 15^th^.

**4. Spawning Frequency** - Once per week or as required (deceased
females will not be spawned). Schedule: Tuesday-IM, Wednesday-LR, LGCR,
Thursday-UGR, CC.

**5. Spawning Strategies** - All spawning will be done at Lookingglass
Hatchery. Sorting and spawning to take place the same day. Hatchery and
co-manager staffs will determine fertilization matrices. Most spawning
matrices will be 2x2, but matrices of 1 female x 2 males or 1x1 or 2x1
can be used if necessary. Fertilized eggs will be incubated at
Lookingglass hatchery. Fecundity will be determined at eye-up. If a ripe
female is observed during sorting and no ripe male is available, the
female will be returned to the holding pond until a ripe male is
located. Ripe male gametes can be collected in an emergency (priority
intended):

> **a. Sperm on ice** from fish passed at weirs - These fish will be
> given a 1LOP opercle punch so they can be identified during spawning
> surveys and counted as "taken".
>
> **b. Cryopreserved sperm** Fill out request form (Appendix G).
>
> **c. If milt is not available after 7 days** of holding a ripe female,
> transport female(s) to river of origin.

***Note: Gene Banking** **--** Sperm from males will be cryopreserved on
the day of spawning. NPT will provide staffing and coordination with
Lookingglass Hatchery*.

**[F. BKD Management]{.underline}.**

Progeny are categorized based on their maternal parent. Categories
include:

-   *≤ 0 .199 =Low*

-   *0.2 - 0.399 = Moderate/Low*

-   *0.4 - 0.799 = Moderate/High*

-   *0.800 --above= Clinical*

    *Note -- Production groups are based on ([R.]{.underline}
    [Salmoninarum)]{.underline} antigen levels of the maternal parent.
    Antigen levels are determined by enzyme-linked immunosorbent assay
    (ELISA). Fish Health recommends rearing progeny from parents with
    lowest BKD titer levels or segregated by category*.

**[G. Incubation Strategies]{.underline}** -- All stocks will be
incubated at Lookingglass Hatchery using a combination of chilled well
water and UV treated (\>60,000 uw/cm^2^/sec) creek water.

> **1. Hatchery Program** -- Each female's eggs will be incubated in one
> tray until disease screening profiles results are completed. Eggs
> maybe combine after fecundity estimates are completed. Incubation
> temperatures will be manipulated to bring all spawn dates together
> during incubation and early rearing.

**[H. Early Rearing Program]{.underline}** --

> **1**. **Lookingglass --** Catherine, Grande Ronde, Lostine, and
> Lookingglass (CC captive brood) fry will be loaded at 30 to 50
> thousand per trough.
>
> **2. Segregation of eyed-eggs and progeny will occur based on BKD**
> ELISA values of kidneys from spawned females if space allows.
>
> **3. Catherine Creek, Lostine, and Grande Ronde** smolts produced will
> target 25fpp in October 2008.
>
> **4. Lookingglass Creek** production above 150,000 may be transferred
> to Irrigon for rearing between May and September 2008. Prior to
> steelhead marking at Irrigon, pre-smolts will be transferred back to
> Lookingglass in October 2008 and released form the adult holding pond
> in April 2009.

**[I. Fish Health]{.underline}** Monitoring Plans

**1. Disinfection** and Sanitation Guidelines (Appendix C).

**2. Broodstock** monitoring plan (Appendices E, H and I)

**[J. Key Contacts]{.underline}**

**1. Transportation**

> **a. Facility Operators (NPT and CTUIR)** will coordinate all hauling
> and notify LGH (Lund) of the stock, number being hauled and estimated
> arrival time.
>
> **2. Captive Brood-TOT leader** (Hoffnagle) is responsible for
> providing TOT meeting notes and an agenda for the upcoming meeting.
>
> **3. Adult records (AAT's)** will be completed weekly by ODFW
> (requires timely completion of weekly trapping data).

**[X. Grande Ronde Basin-2007 Captive Brood Spring/Summer Chinook--
Catherine, Grande Ronde & Lostine]{.underline}**

*Smolt production (F~1~) and potential out lets of production will be
consistent with locations identified by co-managers.*

**[A. Allocation]{.underline} --** Production estimates:
(Fecundity=1,100, green egg to smolt survival 45%)

-   LR--- 141♀ = 70,000 smolts

-   CC/Look--- 118♀ = 58,000 smolts

-   UGR--- 140♀ = 69,000 smolts

Note: Production estimates from captive brood program are extremely
variable.

[**B.** **Spawning\--**]{.underline}See Captive Brood 2007 AOP.

**[C. Incubation]{.underline}**

> **1. Captive Brood** incubation to eyed stage at Oxbow Hatchery. After
> eye-up, inventory, and disease profiles, Oxbow staff will combine eggs
> by females, treatments, and BKD level in groups of about 5,000 eggs.
> Eyed eggs will be shipped to Lookingglass Hatchery.

# D. Key Contact

**1.** Captive Brood TOT project leader (Hoffnagle)

**2.** Oxbow Hatchery manager (Banks)

**[XI. Imnaha -2005 Brood Year-Spring/Summer Chinook]{.underline}**

**[A.]{.underline}** **[Anticipated smolt release]{.underline}** -- A
total of 433,000 smolts at 22fpp.

-   Acclimated: 433,000

-   Direct stream: 0

**[B. Liberations]{.underline}** (See Appendix A)

> **1. Transfer and Acclimation** -- Approximately 310,000 smolts (5
> raceways) will be transferred to Imnaha Satellite between March 12 and
> 14 and held for acclimation. Satellite personnel will begin volitional
> release March 21. In late March, the balance (2 raceways) or
> approximately 123,000 smolts will be transferred to acclimation pond.
> The acclimation pond screens will be reset for 2 days. After two days,
> the volitional period will resume. Any remaining fish will be forced
> out on April 12. Release number will be determined by transfer
> inventory minus mortality.

**[C. Imnaha Satellite Operation]{.underline}**

> **1. Schedule and Operations** -- Open road to facility and begin
> set-up the first week in March. Close down facility in late April.

**2. Scan mortality for PIT tags by** ODFW staff

# D. Monitoring and Evaluation

> **1. Imnaha** summary of marked chinook released in 2007.

-   186,000 AdCWT

-   247,000 Ad only

-   21,000 PIT

> **2.** Fish Research staffs will coordinate efforts with hatchery
> staff for pre-release sampling efforts (Tables 2, 3, and 5).

**[E. Fish Health]{.underline}**\-- Fish Health staff will coordinate
efforts with Fish Research and hatchery staffs to conduct pre-release
health examines (Appendix D). Standard disinfection and sanitation
guidelines will apply (Appendix C).

**[F. Key Contacts]{.underline}**

> **1. ODFW** (Lund) will notify NPT (B. Johnson, Hesse, Harbeck, Vogel
> (208.816-1405), Michaels (426-3198)), ODFW (D. Eddy, Monzyk,
> Hoffnagle), LSRCP office and FPC (TBA) of date and numbers of fish
> release.

**[XII. Imnaha --2006 Brood Year-Spring/Summer Chinook]{.underline}**

> **1. Smolt Production-** An estimated 360,000 smolts will be produced
> at a target size of 22fpp in mid-March 2008.
>
> **a. Early** Rearing -- Fry will be reared in 8 double deep troughs at
> Lookingglass Hatchery on UV treated water Lookingglass Creek water.
> Fish will be fed a 14-day "pro-active" feed in lieu of a 28-day
> medicated feed treatment prior to transferring outside. Fish will be
> transfer outside to raw creek water in April or May.
>
> **b. Final Rearing -** After marking, fish will be divided into 6
> raceways with approximately 60,000 fish per raceway (Appendix F). In
> August, a prophylactic feed treatment will be administered to control
> BKD.

**[B. Monitoring and Evaluation]{.underline}**

> **1. Fish marking-** All fish will be ad clipped in June and July
> 2007. Approximately 180,000 fish will receive CWT (Table 6).

**2. PIT tag**- 21,000 fish will be PIT tagged in October 2007 (Table
8).

[**C. Marking Program** --]{.underline}

**1. AdCWT** -- 180,000

**2. AD-** 180,000 .

**3. Pit tag** --21,000 .

**[E. Key Contacts]{.underline}**

> **1. Lookingglass** (Lund)
>
> **2. Fish Health** (Onjukka)
>
> **3. Fish Research** (Monzyk and Vogel)

**[XIII. Imnaha --2007 Brood Year-Spring/Summer Chinook]{.underline}**

*The production goal is 360,000 smolts for the Imnaha River reared in 6
raceways. NEOH the long- term goal will be to produce 490,000 smolts.*

**[A. Smolt goal -]{.underline}** 360,000 smolts at 20fpp for release
2009.

**[B. Adult Collection]{.underline}**

> **1. Predicted Runs-** Total estimated return to river is 956 adults.
> The total with jacks is 1,348 fish. This includes 1,021 hatchery
> produced (males and females) and 327 naturally produced (males and
> females) fish. Approximately 51% of fish entering the Imnaha River are
> expected to be collected at the weir and the collection of adults is
> estimated at: 501 hatchery-origin (males, females) and 167
> natural-origin (males and females) (Table 12).

# 

# C. Trap Operations

**1. Period of Trap Operation** -- Install trap as soon as river
conditions allow and operate until September 11.

**2. Trap/sorting Frequency---**The trap will be worked on Mondays or
more often if needed.

**3. Disposition of Trapped Fish**

**a. Adults and jacks** --Fish retained for broodstock, will be injected
intraperitoneally (IP) with erythromycin and oxytetracycline and opercle
punched (Appendix I). If sport or Tribal fishery occurs, only fish
retained for broodstock will be injected. Hatchery-origin jacks and
hatchery-origin adults, collected above broodstock needs, can be held at
Wallowa Hatchery for 21-days and provided to the Tribes for C/S.

> **b. Tumors** **-** Fish will be inspected for tumors along the gum
> line. If a tumor is suspected, fish with will be held for
> consultation.

c.  **Adults for outplanting** in Big Sheep and Lick Creek. Up to 300
    hatchery-origin adults can be outplanted. Adults collected prior to
    July 16^th^ and targeted for outplanting, will be held at
    Lookingglass Hatchery. Adults collected after July 16^th^, and
    targeted for outplanting, can be direct stream released.

> ***Note:** ODFW has proposed using surplus adults for a jack spawning
> study on Lick Creek. The projected levels suggest 27 adults may be
> available. This proposal requires additional consultation.*
>
> **d. Disposition of Carcasses***.* Trapping mortalities will be
> processed as kept fish and the carcasses provided to Fish health for
> examination, if possible. Other pre-spawning mortalities discovered
> during weir-effect surveys will also be sent to Fish Health.
> Biological data will be sent to ODFW Fish Research (Monzyk). Following
> examination, the carcasses may be disposed of by Tribal distribution,
> habitat or landfill.

### 

### D. Hatchery Broodstock Collection Guidelines

**1. Weir management---**At 956 adult estimated escapement, the sliding
scale guidelines suggests that: ≤70% of the fish released above the weir
may be of hatchery origin; ≥25% broodstock of natural origin; take ≤40%
of the wild return for brood; 10% of the males above the weir may be
age-3 hatchery males.

**2. Broodstock Needs**

> **a. Egg take-** Need 450,000 green eggs at 80% survival from green
> egg to smolt and estimated five-year fecundity average of 4,826.

**b. Adult Collection**- Based on adult survival of 90%:

Males -- 104 (spawn 93)

-   26 natural (spawn 24)

-   78 hatchery (spawn 69-6 jacks equal one male)

Females -- 104 (spawn 93)

-   26 natural (spawn 24)

-   78 hatchery (spawn 69)

**3. Brood collections guidelines**: The current projection for adult
spring/summer chinook returns to Imnaha River is 956 adults: 706
ad-clipped and 250 unmarked fish. However, it is expected that only 51%
of the run will be intercepted at the weir. Fish released above the weir
will be managed below or equal to 70% hatchery to 30% wild origin ratio.

**Estimated Totals: Estimate 51% Escapement**

**Escapement to mouth collected Broodstock OP above the weir**

706-Hatchery 360 156 27 177

250-Wild 128 52 0 76

Collections will be based on the following:

+-----------+-----------+-----------+-----------+-----------+-----------+
| # Co      |           |           |           |           |           |
| llection  |           |           |           |           |           |
| guideline |           |           |           |           |           |
| s for Imn |           |           |           |           |           |
| aha sprin |           |           |           |           |           |
| g chinook |           |           |           |           |           |
|  in 2007. |           |           |           |           |           |
+-----------+-----------+-----------+-----------+-----------+-----------+
|           | June 1-22 | June      | July 1-8  | July 9-16 | July      |
|           |           | 23--30    |           |           | 17-23     |
+-----------+-----------+-----------+-----------+-----------+-----------+
| HOB-156   | 4         | 16        | 36        | 32        | 24        |
+-----------+-----------+-----------+-----------+-----------+-----------+
| NOB-52    | 4         | 2         | 14        | 12        | 8         |
+-----------+-----------+-----------+-----------+-----------+-----------+
| Outplants |           | up to 30  | up to 10  | up to 10  | up to 0   |
+-----------+-----------+-----------+-----------+-----------+-----------+

# 

+-----------+-----------+-----------+-----------+-----------+-----------+
| #         | #         | # A       | # Au      | # Aug     | # Se      |
| July24-31 | Aug 1 - 8 | ug 9 - 16 | g 17 - 23 | 24 - Sep  | pt 1 - 15 |
+-----------+-----------+-----------+-----------+-----------+-----------+
| # 14      | # 12      | # 4       | # 4       | # 8       | # 2       |
+-----------+-----------+-----------+-----------+-----------+-----------+
| # 6       | # 2       | # 2       | # 0       | # 2       | # 0       |
+-----------+-----------+-----------+-----------+-----------+-----------+

\*Release all wild jacks

\*\*Retain all hatchery-produced jacks until the run is reassessed in
late July.

> The intent is to collect brood stock in pairs or female and jack-male
> equivalent. Six hatchery jacks is the equivalent of one male. If adult
> and jack return numbers exceed brood stock and outplant needs, the
> balance will be transferred to Wallowa Hatchery for Tribal C/S. Jacks
> and surplus adults should not be transferred to Wallowa Hatchery after
> August 8.

**4. Natural escapement projections\--**A total of 748 adults (26.5%
wild) are prognosticated to spawn in the Imnaha sub-basin, 721 in the
Imnaha River and 27 in Big Sheep and Lick Creek. Natural-origin fish
returning to Big Sheep and Lick Creek is unknown. Estimates do not
include harvest or jacks.

**[E. Spawning Guidelines]{.underline}**

**1. Anesthetic Used** -- Aqui-S.

**2. Expected First Spawn** - Tuesday, August 14.

**3. Spawning Frequency** -- Once per week.

**4. Spawning Strategies** - All salmon spawned will be incubated at
Lookingglass Hatchery.

**[F. Incubation]{.underline}**

> **1. Imnaha eggs** will be incubation to eyed stage at Lookingglass
> Hatchery. The intent is to incubate one female's eggs per tray. After
> eye-up, eggs will be enumerated and segregated by disease profile.

**2. Water Sources** --Lookingglass-chilled well water, and UV treated
Lookingglass Creek.

**[G. Fish Health Monitoring plans]{.underline}**

> **1. Disinfection** and Sanitation Guidelines (Appendix D).
>
> **2. Broodstock** monitoring plan (Appendices E, H and I)

**[H. Imnaha Fishery Proposal Summary]{.underline}** --The projected
return of natural fish is 327 in which 250 are adults. At this level of
wild fish escapement, no recreational fishery is proposed.

**[I. Key Contacts]{.underline}**

> **1. Lookingglass** (Lund) monthly reports to ODFW (La Grande &
> Wallowa fish districts and Hoffnagle), CTUIR (Zimmerman and McLean),
> NPT (B. Johnson, Hesse, Harbeck, Zollman), LSRCP office.
>
> **2. Fish Health** (Onjukka) monthly reports to ODFW (La Grande &
> Wallowa fish districts, and Research), CTUIR (Zimmerman and McLean),
> NPT (B. Johnson, Hesse, Harbeck, Zollman).
>
> **3. Fish Research** (Monzyk) monthly reports to ODFW (La Grande &
> Wallowa fish districts, and Hoffnagle), CTUIR (Boe, James, McLean),
> NPT (B. Johnson, Hesse, Vogel, Zollman).

  -------------------- -- --------------------- -- -------------------- -- ------------------ -- ---------------------- -- ---------------------- --
  Table 1. Irrigon                                                                                                                                
  Hatchery Transfer                                                                                                                               
  schedule, 2007.                                                                                                                                 

                                                                                                                                                  

  (06 brood)                                                                                                                                      

                                                                                                                                                  

  [Date]{.underline}      [Stock]{.underline}      [From                   [To]{.underline}      [Number]{.underline}      [Est.                  
                                                   Ponds]{.underline}                                                      Pounds]{.underline}    

                                                                                                                                                  

  Feb.14-20               5606                     7\*,8,9\*,10,11\*       Wallowa Lower Acc     180,000                   40,000                 

                                                                                                                                                  

  Feb. 20-23              5606                     12,13\*, 14\*,15\*      Wallowa Upper Acc     180,000                   40,000                 

                                                                                                                                                  

  Mar. 5,6                5606                     16\*,17,                Big Canyon Upper      80,000                    17,778                 
                                                                           Acc                                                                    

                                                                                                                                                  

  Mar. 6,7                5606                     18, 19,                 Big Canyon Lower      80,000                    17,778                 
                                                                           Acc                                                                    

                                                                                                                                                  

  Mar. 7-9                2906                     25,26,27\*,29           Little Sheep Acc      165,000                   33,000                 

                                                                                                                                                  

  Apr. 9-11               2906                     28, 30                  Big Sheep (Direct)    100,000                   20,000                 

                                                                                                                                                  

  Apr. 16-18              5606                     5\*, 6, 21\*            Wallowa Lower Acc     120,000                   26,667                 

                                                                                                                                                  

  Apr. 18-19              5606                     20, 22,                 Big Canyon Lower      80,000                    17,778                 
                                                                           Acc                                                                    

                                                                                                                                                  

  Apr. 19-20              5606                     23\*,24                 Big Canyon Upper      [80,000]{.underline}      [17,778]{.underline}   
                                                                           Acc                                                                    

                                                                                                 1,065,000                 230,779                
  -------------------- -- --------------------- -- -------------------- -- ------------------ -- ---------------------- -- ---------------------- --

\~Denotes partial pond

\*Denotes CWT pond

### Table 2. Juvenile spring Chinook salmon and summer steelhead sampling schedule at LSRCP facilities, 2007. PS = Periodic sampling which includes length and weight. RS = Release sampling which includes length and weight by fin clip. CWT = sampling for CWT and VIE retention and associated fin clips. NA indicates not applicable.

Species,

[Sample Date Stock Location Pond Purpose]{.underline}

# *Spring Chinook*

Feb. 6-9 Catherine (05) Lookingglass 1-2 CWT, release samples Feb. 6-9
U. Grande Ronde (05) Lookingglass 3-6 CWT, release samples Feb. 6-9
Lostine (05) Lookingglass 7-11 CWT, release samples

Feb. 6-9 Imnaha (05) Lookingglass 12-18 CWT, release samples

Feb.21-23 Catherine (06) Irrigon Ponding samples

Feb. 21-23 U. Grande Ronde (06) Irrigon Ponding samples

Feb. 21-23 Lostine (06) Irrigon Ponding samples

Feb. 21-23 Lostine (06) Lookingglass Ponding samples

Feb. 21-23 Imnaha (06) Lookingglass Ponding samples

June 7-8 All Lookingglass 1 -- 18 INAD samples

#### *Summer Steelhead (brood 06)*

Jan. 8-9 Wallowa Irrigon 9, 11,13,15 CWT\
Jan. 16-17 Wallowa Irrigon 21, 23, 27 CWT

Jan. 22-23 Wallowa/Imnaha Irrigon 5,7,14,16 CWT\
April 6 Wallowa Wallowa LAP, UAP Release samples\
April 9 Wallowa Big Canyon LAP, UAP Release samples\
April 10 Imnaha Little Sheep AP Release samples\
April 27 Wallowa Wallowa LAP Release samples

May 11 Wallowa Big Canyon LAP Release samples\
April 30 Wallowa Big Sheep LAP, UAP Release samples

Table 3. Genetics Monitoring Sampling for spring Chinook brood year 2005
and summer Steelhead brood year 2006, both released in 2007.

[Sample Date Stock Location Pond Number of Fish]{.underline}

# *Spring Chinook*

Feb. 7-9 Catherine Lookingglass 1-2 50

Feb. 7-9 Grande R Lookingglass 3-6 50

Feb. 7-9 Lostine Lookingglass 7-11 50

Feb. 7-9 Imnaha Lookingglass 12-18 50

#### *[Summer Steelhead]{.underline}*

April 9 Wallowa Big Canyon LAP 50

April 10 Imnaha Little Sheep AP 50

Table 4.

Summer Steelhead run projections to LSRCP Facilities in 2007.

**2006 PROJECTED Returns to Wallowa Hatchery**

**MARKED FISH**

[Age Males Females Total 95%C.I.]{.underline}

Marked 1:1 768 492 1,260

Marked 1:2 172 480 652

Marked 2:1 9 8 17 Marked 2:2 1 2 3 Total 950 982 1,932 (585 - 3,279)

**2006 PROJECTED Returns to Big Canyon Satellite**

**MARKED AND UNMARKED FISH**

[Age Males Females Total 95%C.I.]{.underline}

Marked 1:1 375 350 725

Marked 1:2 80 276 356

Marked 2:1 3 4 7

subtotal 458 630 1,088 (58 - 2,119)

UnMarked 2:1 13 16 31

UnMarked 2:2 7 14 23

UnMarked 3:1 13 10 22

UnMarked other 5 9 11

subtotal 40 47 87 (46-129)

Total 498 977 1,175

**2006 PROJECTED Returns to Little Sheep Creek Satellite MARKED AND
UNMARKED FISH**

[Age Males Females Total 95%C.I.]{.underline}

Marked 1:1 628 601 1,229

Marked 1:2 79 309 388

Marked 2:1 11 7 18

Marked 3:1 0 2 2

subtotal 718 919 1,637 (510 - 2,849)

UnMarked 1:2 0 0 0

UnMarked 2:1 19 29 49

UnMarked 2:2 4 15 19 UnMarked 3:1 8 9 16

UnMarked 3:2;4:1 2 4 6

subtotal 33 57 90 (1-180)

Total

Table 5. Summary of projected number of tagged releases for the 2007
brood summer steelhead and 2006 brood spring Chinook salmon.

Species, Number Type of Marking Marking

Stock Marked Mark Period Location

**Summer Steelhead**

2007 Brood Year (Ad and RV clips in September)

Little Sheep 25,000 Ad-LV+CWT November Irrigon

Wallowa 150,000 Ad-LV+CWT November Irrigon

Wallowa 100,000 Ad-RV+CWT November Irrigon

**Spring Chinook Salmon**

# *2006 Brood Year (Ad clips in June/July)*

Imnaha River 180,000 Ad+CWT June/July Lookingglass

180,000 Ad only June/July Lookingglass

Catherine CB ~mod/lo~ 28,000 Ad+CWT June/July Lookingglass

Catherine CB ~mod/hi~ 20,000 Ad+CWT June/July Lookingglass

Lostine 198,000 Ad+CWT June/July Lookingglass

Lostine 14,000 CWT only June/July Lookingglass

Upper GR~.~ 130,000 June/July Lookingglass

Upper GR. 130,000 June/July Lookingglass

Catherine 60,000 Ad+CWT June/July Lookingglass

60,000 Ad only June/July Lookingglass

## 

Table 6. PIT-tagging schedule for 2007 brood summer steelhead at Irrigon
Hatchery scheduled over three week period in November 2007. Fish need to
be off feed 2 days prior to and after PIT-tagging to reduce tag loss. In
a given week, 2-4 raceway groups will be hauled to inside tanks on
Fridays; PIT tagged the following Monday and/or Tuesday and returned to
raceways Wednesday and Thursday. False bottoms will be used to recover
shed tags.

Experimental group Number to PIT tag Number in tank

  -------------------------------------- ----------------- --------------
  Wallowa production, forced April       900               950
  release                                                  

  Wallowa production, forced April       900               950
  release                                                  

  Wallowa production, forced April       900               950
  release                                                  

  Wallowa production, volitional May     900               950
  release                                                  

  Wallowa fallbrood, forced April        900               950
  release                                                  

  Wallowa fallbrood, forced April        900               950
  release                                                  

  Wallowa fallbrood, forced April        900               950
  release                                                  

  Wallowa fallbrood, volitional May      900               950
  release                                                  

  Big Canyon, production, forced April   300               350
  release                                                  

  Big Canyon, production, volitional May 300               350
  release                                                  

                                                           

  Subtotal                               7,800             8,300

                                                           

  Little Sheep, production, volitional   300               350
  April release                                            

  Big Sheep supplementation, direct      300               350
  stream April release                                     

                                                           

  Subtotal                               600               700

                                                           

  Grand total                            8,400             9,000

                                                           
  -------------------------------------- ----------------- --------------

^A^ PIT-tag a random sample by crowding each raceway to obtain target
number. When tagging, note whether the fish is AdLV, AdRV, Ad-only, or
no clip.

*Table 7. Fish PIT-tagging numbers for spring Chinook salmon at
Lookingglass Fish Hatchery, October 2007 (BY2006). Note: Fish must be
off feed 2 days prior and 2 days after PIT tagging to reduce tag loss.*

  ---------------------------- --------- ----------------- -----------------
  Experimental group           Raceway   Estimated \# per  Number to PIT tag
                                         raceway           

  Catherine Conventional       1         60,000            10,500

  Catherine Conventional       2         60,000            10,500

  Catherine Creek              3         28,000            1,000
  CBS/Lookingglass Cr                                      

  Catherine Creek              4         20,000            1,000
  CBS/Lookingglass Cr                                      

  U. Grande Ronde Conventional 5         65,000            500

  U. Grande Ronde Conventional 6         65,000            500

  U. Grande Ronde Conventional 7         65,000            500

  U. Grande Ronde Conventional 8         65,000            500

  Lostine Conventional         9         66,000            1,667

  Lostine Conventional         10        66,000            1.667

  Lostine Conventional         11        66,000            1,667

  Lostine CBS                  12        14,000            1,500

  Imnaha                       13        60,000            3,500

  Imnaha                       14        60,000            3,500

  Imnaha                       15        60,000            3,500

  Imnaha                       16        60,000            3,500

  Imnaha                       17        60,000            3,500

  Imnaha                       18        60,000            3,500

  Grand total                            1,000,000         50,500
  ---------------------------- --------- ----------------- -----------------

Table 8. Projected spring Chinook salmon returns to Catherine Creek in
2007.

  ---------- ------- --------- --------- ------------ -------- ---- ---- --------- ---------
  Mark       Age     Males     Females   Total        95%CI                        

  **Return                                                                         
  to River**                                                                       

  Marked     3       17        0         17           1        to                  33

  Marked     4       19        22        41           4        to                  78

  Marked     5       16        9         25           0        to                  61

  Total              **52**    **31**    **83**       **5**    to                  **172**

  Unmarked   3       14        0         14           0        to                  31

  Unmarked   4       78        64        142          0        to                  292

  Unmarked   5       21        19        40           1        to                  79

  Total              **113**   **83**    **196**      **1**    to                  **402**

  Grand              **165**   **114**   **279**      **6**    To                  **574**
  Total                                                                            

  **Total to                                                                       
  weir**                                                                           
  (87% of                                                                          
  run                                                                              
  trapped at                                                                       
  weir -                                                                           
  five year                                                                        
  average)                                                                         

                     **144**   **99**    **243**      **5**         to   **499**   
  ---------- ------- --------- --------- ------------ -------- ---- ---- --------- ---------

Table 9. Projected spring Chinook salmon returns to the Upper Grande
Ronde in 2007.

  ---------- ------- -------- --------- ------------ -------- ---- ---- --------- ---------
  Mark       Age     Males    Females   Total        95%CI                        

  **Return                                                                        
  to River**                                                                      

  Marked     3       3        0         3            0        to                  8

  Marked     4       9        11        20           0        to                  50

  Marked     5       31       11        42           0        to                  122

  Total              **43**   **22**    **65**       **0**    to                  **180**

  Unmarked   3       17       0         17           0        to                  43

  Unmarked   4       17       13        30           0        to                  92

  Unmarked   5       21       17        38           0        to                  111

  Total              **55**   **30**    **85**       **0**    to                  **247**

  Grand              **98**   **52**    **150**      **0**    To                  **427**
  Total                                                                           

  **Total to                                                                      
  weir**                                                                          
  (80% of                                                                         
  run                                                                             
  trapped at                                                                      
  weir -                                                                          
  five year                                                                       
  average)                                                                        

                     **78**   **42**    **120**      **0**         to   **342**   
  ---------- ------- -------- --------- ------------ -------- ---- ---- --------- ---------

Table 10. Projected spring Chinook salmon returns to the Lostine River
in 2007.

  ---------- ------- --------- --------- ------------ --------- ---- ---- ----------- -----------
  Mark       Age     Males     Females   Total        95%CI                           

  **Return                                                                            
  to River**                                                                          

  Marked     3       165       2         167          0         to                    371

  Marked     4       50        62        112          29        to                    196

  Marked     5       27        20        47           0         to                    123

  Total              **242**   **84**    **326**      **29**    to                    **690**

  Unmarked   3       47        1         48           24        to                    71

  Unmarked   4       219       226       445          267       to                    622

  Unmarked   5       38        22        60           7         to                    114

  Total              **304**   **249**   **553**      **298**   to                    **807**

  Grand              **546**   **333**   **879**      **326**   To                    **1,497**
  Total                                                                               

  **Total to                                                                          
  weir**                                                                              
  (69% of                                                                             
  run                                                                                 
  trapped at                                                                          
  weir -                                                                              
  five year                                                                           
  average)                                                                            

                     **377**   **230**   **607**      **225**        to   **1,033**   
  ---------- ------- --------- --------- ------------ --------- ---- ---- ----------- -----------

## Table 11. Projected spring chinook salmon returns to Lookingglass Fish Hatchery in 2007.

  ---------------------------- ------------------ ------------------------
  **Mark**                     **Age**            **Total**

  Marked (CC)                  **3**              28

  Marked (CC)                  **Adult**          435

                                                  

  UnMarked                     **3**              2

  UnMarked                     **Adult**          40

                               **Total**          **505**

                                                  
  ---------------------------- ------------------ ------------------------

Table 12. Projected spring Chinook salmon returns to the Imnaha River in
2007.

  ---------- ------- --------- --------- ------------ --------- ---- ---- --------- -----------
  Mark       Age     Males     Females   Total        95%CI                         

  **Return                                                                          
  to River**                                                                        

  Marked     3       315       0         308          115       to                  514

  Marked     4       200       198       399          274       to                  522

  Marked     5       120       188       308          80        to                  536

  Total              **635**   **386**   **1,021**    **469**   to                  **1,572**

  Unmarked   3       77        0         77           11        to                  143

  Unmarked   4       70        38        108          79        to                  137

  Unmarked   5       61        81        142          80        to                  204

  Total              **208**   **119**   **327**      **170**   to                  **484**

  Grand              **843**   **505**   **1,348**    **639**   To                  **2,056**
  Total                                                                             

  **Total to                                                                        
  weir**                                                                            
  (51% of                                                                           
  run                                                                               
  trapped at                                                                        
  weir -                                                                            
  five year                                                                         
  average)                                                                          

                     **430**   **258**   **688**      **276**        to   **793**   
  ---------- ------- --------- --------- ------------ --------- ---- ---- --------- -----------

  -------------- --------- -------- ---------- ----------- --------- ------ ----------- ---------- --------- ------------------- ------------ -------------
  APPENDIX A.                                                                                                                                 
  PROPOSED                                                                                                                                    
  JUVENILE                                                                                                                                    
  SALMONID                                                                                                                                    
  RELEASES IN                                                                                                                                 
  THE GRANDE                                                                                                                                  
  RONDE (GR) AND                                                                                                                              
  IMNAHA (IM)                                                                                                                                 
  BASINS IN 2007                                                                                                                              

                                                                                                                                              

                                                                                                                                              

  Basin          Species   Stock    Hatchery   Number      Lbs       fpp    Location    In         In River  Pond \# ^(/2)^      Release      Marks
                                               ^(/1)^                                   Facility                                 Method       
                                                                                                                                 ^(/3)^       

  GR             STS       5606     IR         180,000     40,000    4.5    Wallowa     Feb 14-20  Apr 8-9   7\*,8,9\*,10,11\*   Forced       75K AdRVCWT;
                                                                            Lower Acc                                                         75K AdLVCWT;
                                                                                                                                              210K Ad only,

  GR             STS       5606     IR         180,000     40,000    4.5    Wallowa     Feb 20-23  Apr 9-10  12,13\*, 14\*, 15\* Forced       
                                                                            Upper Acc                                                         

  GR             STS       5606     IR         80,000      17,778    4.5    Big Canyon  Mar. 5, 6  Apr 11-12 18,19               Forced       80K Ad
                                                                            Lower                                                             

  GR             STS       5606     IR         80,000      17,778    4.5    Big Canyon  Mar. 6, 7  Apr 12-13 16\*,17             Forced       25K AdLVCWT;
                                                                            Upper                                                             55K Ad

  IM             STS       2906     IR         165,000     33,000    5.0    Little      Mar 7, 9   Apr 10-   25,26,27\*, 29      Volitional   25K AdLVCWT;
                                                                            Sheep Acc              May 1                                      140K Ad

  IM             STS       2906     IR         100,000     20,000    5.0    Big Sheep   NA         Apr 9-11  28, 30              Direct       100K no mark
                                                                            Cr                                                   Stream       

  GR             STS       5606     IR         120,000     26,667    4.5    Wallowa     Apr. 16-18 Apr       5\*,6, 21\*         Volitional   25K AdLVCWT;
                                                                            Lower Acc              28-May 10                                  25KAdRVCWT;
                                                                                                                                              70K Ad

  GR             STS       5606     IR         80,000      17,778    4.5    Big Canyon  Apr 18-19  May 1-May 20, 22              Volitional   80K Ad
                                                                            Lower Acc              11                                         

  GR             STS       5606     IR         80,000      17,778    4.5    Big Canyon  Apr 19-20  May 2-May 23\*,24             Volitional   25K AdLVCWT;
                                                                            Upper Acc              11                                         55K Ad

                                               1,065,000   230,779   4.61                                                                     

                                                                                                                                              

  GR             CHS       80F05    LG         20,600      936       22     Grande      Mar. 13-14 Mar.-26   6                   Volitional   AdCWT 100%
                                                                            Ronde Acc              Apr 11                                     

  GR             CHS       8005     LG         119,000     5,409     22     Grande      Mar 13     Mar 26-   3,4,5               Volitional   CWT 100%
                                                                            Ronde Acc              Apr 11                                     

  GR             CHS       20005    LG         105,300     4,786     22     Lostine Acc Mar 5-6    Mar 16-26 8,10                Volitional   AdCWT 100%,

  GR             CHS       200F05   LG         24,700      1,123     22     Lostine Acc Mar 27-28  Apr 7-17  11                  Volitional   AdCWT 100%,
                                                                                                                                              VIE

  GR             CHS       20005    LG         100,400     4,564     22     Lostine Acc Mar 27, 28 Apr 7-17  7, 9                Volitional   AdCWT 100%

  GR             CHS       20105    LG         49,800      2,264     22     Catherine   Mar 12     Mar.26-   1                   Volitional   AdCWT 100%
                                                                            Cr Acc                 Apr 11                                     

  GR             CHS       201F05   LG         21,500      977       22     Catherine   Mar 12     Mar.26-   2                   Volitional   AdCWT 100%,
                                                                            Cr Acc                 Apr 11                                     VIE

  IM             CHS       2905     LG         433,000     19,682    22     Imnaha Acc  March      Mar.      12-18               Volitional   250K Ad; 183K
                                                                                        12-14      21-Apr 12                                  AdCWT

                                                                                                                                              

                                               874,100     39,741    22.0                                                                     

  ^(/1)^ Numbers                                                                                                                              
  of fish based                                                                                                                               
  on recent                                                                                                                                   
  hatchery                                                                                                                                    
  estimates, not                                                                                                                              
  AOP goal                                                                                                                                    
  numbers                                                                                                                                     

  ^(/2)^ \*                                                                                                                                   
  Indicates                                                                                                                                   
  AdLVCWT or                                                                                                                                  
  AdRVCWT group                                                                                                                               
  in pond                                                                                                                                     

  ^(/3)^ Forcing                                                                                                                              
  occurs                                                                                                                                      
  following a                                                                                                                                 
  minimum 24 hr.                                                                                                                              
  volitional                                                                                                                                  
  opportunity.                                                                                                                                
  Volitional and                                                                                                                              
  forced                                                                                                                                      
  releases are                                                                                                                                
  all                                                                                                                                         
  acclimated.                                                                                                                                 
  -------------- --------- -------- ---------- ----------- --------- ------ ----------- ---------- --------- ------------------- ------------ -------------

## Appendix B. Steelhead Fish Health Monitoring Plan & Disease Treatments 

+-----+----+---+-----+--------+--------------------+-----------------+
| Lo  | B  | S | St  | Exami  | Protocol           | Comment         |
| cat | ro | p | ock | nation |                    |                 |
| ion | od | e |     | Ca     |                    |                 |
|     | ye | c |     | tegory |                    |                 |
|     | ar | i |     |        |                    |                 |
|     |    | e |     |        |                    |                 |
|     |    | s |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| I   | 20 | S | W   | M      | -10 mort/moribund  | Treat with      |
| rri | 06 | t | all | onthly | per stock examined | Florfenicol     |
| gon | &  | S | owa | &      |                    | under INAD if   |
| Ha  | 20 |   | (   | P      | -kidney smears on  | CWD losses      |
| tch | 07 |   | 56) | relibe | TYE-S agar         | become a        |
| ery |    |   | and | ration |                    | problem         |
|     |    |   | Lit |        | -Gill culture      |                 |
|     |    |   | tle |        | smears on agar if  |                 |
|     |    |   | Sh  |        | suspect gill       |                 |
|     |    |   | eep |        | disease            |                 |
|     |    |   | (   |        |                    |                 |
|     |    |   | 29) |        | -Gill and skin wet |                 |
|     |    |   |     |        | mounts from a      |                 |
|     |    |   |     |        | combination of     |                 |
|     |    |   |     |        | moribund and       |                 |
|     |    |   |     |        | healthy fish       |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| I   | 20 | S | 56  | Annual | 60 smolts that     | Prefer using    |
| rri | 06 | t | or  | Myx    | have been on the   | saved           |
| gon |    | S | 29  | obolus | water supply for   | mortalities     |
| Ha  |    |   |     | cere   | at least 6 months  |                 |
| tch |    |   |     | bralis |                    |                 |
| ery |    |   |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| Ste | 20 | S | 56  | P      | Steelhead          | Fish Health     |
| elh | 06 | t | &   | relibe | acclimated more    | guidelines are  |
| ead |    | S | 29  | ration | than 3 weeks will  | that these      |
| ac  |    |   |     |        | be monitored as in | non-migrants    |
| cli |    |   |     |        | monthly protocol   | (infected with  |
| mat |    |   |     |        | above              | the agent of    |
| ion |    |   |     |        |                    | Whirling        |
| si  |    |   |     |        |                    | Disease) should |
| tes |    |   |     |        |                    | not be stocked  |
| --  |    |   |     |        |                    | to other areas  |
| WA, |    |   |     |        |                    |                 |
| BI  |    |   |     |        |                    |                 |
| &   |    |   |     |        |                    |                 |
| LI  |    |   |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| W   |    | R |     | Annual | Will need to rear  | Must be on      |
| all |    | b |     | Myx    | Rb on each water   | water supply    |
| owa |    |   |     | obolus | supply (well and   | for 6 months    |
| Ha  |    |   |     | cere   | spring) -- brought |                 |
| tch |    |   |     | bralis | in as eyed eggs    |                 |
| ery |    |   |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
|     |    |   |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| W   | 20 | S | 56  | Adult  | Minimum of 60 per  | A weekly sample |
| all | 07 | t | &   | Sp     | stock for          | (N=24) of       |
| owa |    | S | 29  | awners | culturable viruses | ovarian or milt |
| &   |    |   |     |        | (30 from fall      | fluid may be    |
| Lit |    |   |     |        | collected fish)    | sampled.        |
| tle |    |   |     |        | using ovarian      |                 |
| Sh  |    |   |     |        | fluid and          |                 |
| eep |    |   |     |        | c                  |                 |
|     |    |   |     |        | aeca/kidney/spleen |                 |
|     |    |   |     |        | sample pools not   |                 |
|     |    |   |     |        | to exceed 5 fish   |                 |
|     |    |   |     |        | per pool.          |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| W   | 20 | S | 56  | Adult  | -kidney smears on  | Save fall brood |
| all | 07 | t | &   | Mor    | TYE-S agar         | mortalities as  |
| owa |    | S | 29  | tality |                    | well for        |
| &   |    |   |     |        | -A minimum of 20   | examination     |
| Lit |    |   |     |        | or all mortality   |                 |
| tle |    |   |     |        | less than 20 will  |                 |
| Sh  |    |   |     |        | be examined        |                 |
| eep |    |   |     |        |                    |                 |
+-----+----+---+-----+--------+--------------------+-----------------+
| Loo | 20 | S |     | Adults | -mortalities       | The scope of    |
| kin | 07 | t |     |        | examined for       | what can be     |
| ggl |    | S |     |        | culturable         | learned from    |
| ass |    | o |     |        | viruses, bacteria, | these           |
| Cr  |    | r |     |        | R. salmoninarum by | mortalities     |
| eek |    | o |     |        | ELISA              | will depend on  |
|     |    | t |     |        |                    | the degree of   |
|     |    | h |     |        | -If possible viral | degradation.    |
|     |    | e |     |        | samples (ovarian   |                 |
|     |    | r |     |        | fluid or milt)     |                 |
|     |    | s |     |        | will be taken from |                 |
|     |    |   |     |        | "ripe" steelhead   |                 |
|     |    |   |     |        | passed above       |                 |
|     |    |   |     |        | Lookingglass       |                 |
|     |    |   |     |        | Hatchery.          |                 |
+-----+----+---+-----+--------+--------------------+-----------------+

**Appendix C (page 1 of 3): Disinfections and Sanitation Guidelines for
all LSRCP Hatcheries**

**Goal:** To bring all individuals involved in activities at [all LSRCP
facilities]{.underline} in the Grande Ronde and Imnaha Basin program to
an understanding of what is expected and what is reasonable in
minimizing infectious disease risk factors. **Prevention** of infectious
fish disease problems is the overall goal.

**Background:** Since the La Grande Fish Pathology Laboratory was
established in 1987, there have been disinfection and procedural
recommendations made when needed by the responsible fish pathologist. In
1998 and 1999 two documents (cited below) summarized fish disease data
and included recommendations to reduce the impacts of infectious
disease. The concepts behind most of the specific operational
recommendations included in the attachment (page 2) are taken from these
documents. In 1999 there were efforts made through placards and the AOP
process to alert everyone to a higher standard of sanitary practices for
disease prevention at Lookingglass Hatchery. Given that Lookingglass
Hatchery was and still is used as a fish culture facility for multiple
ESA programs, an increased awareness and application of **Quarantine
Mode of Operation** should exist. With the passage of anadromous adults
and the presence of resident fish above Lookingglass Hatchery there is
the certain risk of pathogen introduction into the creek water supply.
Aside from the Lookingglass Hatchery situation, it is important to note
that the statewide fish health management policy (September 12, 2003)
states that preventative and therapeutic fish health strategies must be
implemented at all facilities. The recommended guidelines in this AOP
should be consistent with this state policy and be standard practice at
all LSRCP facilities.

Groberg, W., S.T. Onjukka, and K.A. Brown. July 22, 1998. A Synospsis of
Infectious Disease in Fish at Lookingglass Hatchery.

Groberg, W., S.T. Onjukka, K.A. Brown and R.A. Holt. November 30, 1999.
A Report of Infectious Disease Epidemiology among Spring Chinook Salmon
at Lookingglass Hatchery.

**Definitions:**

**Quarantine** - At Lookingglass Hatchery and other LSRCP facilities
there are multiple programs on station. Each program and raceway within
each program must be treated as an isolated unit with the goal to
prevent cross-contamination with fish pathogens among the many ESA and
non-ESA stocks. All personnel (ODFW, Tribal, volunteers and the visiting
public) need to be aware of these guidelines for maximum disease
prevention benefit.

**Disinfection -** A process that substantially reduces or completely
eliminates all pathogenic microorganisms except spores. The possibility
of a disinfected object transmitting disease-producing organisms is
greatly reduced.

Examples: Disinfection of gear and equipment (boots, bibs or raingear,
nets, crowders, raceways, lib trucks, PIT tag needles). Note:
Disinfection [only occurs if proper procedures are]{.underline}
[implemented]{.underline} to maintain proper concentration of
disinfectant and exposure time.

**Sanitation --** A process that brings microbial contamination to a
"safe" level.

Examples: Quick sanitation (decontamination step) -- use of footbaths
while moving from one area to another, dipping hands in a tub of
iodophor disinfectant, wiping down fish to be spawned with iodophor
prior to spawning.

Note: A summary of recommended disinfectants and for what applications
follows on page 3 of this attachment.

**Appendix C (page 2 of 3): Disinfections and Sanitation Guidelines for
all LSRCP Hatcheries**

**Specific Operational Recommendations**

+------------------+------------------------+-------------------------+
| **Applies to     | **Prevention Control   | **Guideline Comment**   |
| Who?**           | Measure or Sanitary    |                         |
|                  | Practice**             |                         |
+------------------+------------------------+-------------------------+
| All              | Disinfect all          | **-**As per attached    |
|                  | gear/equipment prior   | iodophor protocol       |
|                  | to entering or leaving |                         |
|                  | hatchery grounds       | -Hatchery crew          |
|                  |                        | responsible for         |
|                  |                        | providing tub           |
|                  |                        |                         |
|                  |                        | of 100 ppm iodophor     |
+------------------+------------------------+-------------------------+
| All              | Disinfect equipment    | **-**As per per         |
|                  | when moving from       | attached iodophor       |
|                  | raceway to raceway or  | protocol                |
|                  | tank to tank for       |                         |
|                  | [any]{.underline} fish | -Includes CWTing, fin   |
|                  | handling or pond       | clipping and PIT tag    |
|                  | cleaning activities    | operations. See         |
|                  |                        | footnote for marking\*. |
+------------------+------------------------+-------------------------+
| All              | Use footbaths upon     | -Use larger tub of      |
|                  | entering or leaving    | disinfectant if         |
|                  | the work area for a    | involved in a spawning  |
|                  | given program          |                         |
+------------------+------------------------+-------------------------+
| All              | Use a new disposable   |                         |
|                  | apron or disinfected   |                         |
|                  | personal rain gear     |                         |
|                  | while working with     |                         |
|                  | fish                   |                         |
+------------------+------------------------+-------------------------+
| CTUIR Personnel  | Disinfect all          | -CTUIR personnel        |
| operations at    | gear/equipment prior   | responsible to maintain |
| Lookingglass     | to entering or leaving |                         |
| Hatchery         | hatchery grounds,      | and use a tub of 100    |
|                  | Lookingglass Creek, or | ppm iodophor at intake  |
|                  | the intake building    |                         |
|                  | and when done with     | building workstation    |
|                  | operations at intake   |                         |
+------------------+------------------------+-------------------------+
| Hatchery Crew    | Assure that individual | -All use these for the  |
|                  | raceway and tank       | specifically designated |
|                  | mortality "picker      |                         |
|                  | equipment" is in place | raceway                 |
|                  | at each raceway and    |                         |
|                  | tank                   |                         |
+------------------+------------------------+-------------------------+
| Hatchery Crew    | Sanitize each raceway  | -dry for a minimum of   |
|                  | prior to use for the   | three days              |
|                  | next brood year. (see  |                         |
|                  | page 3 for             |                         |
|                  | recommendation)        |                         |
+------------------+------------------------+-------------------------+
| Hatchery Crew    | Keep footbaths located | -As per iodophor label, |
|                  | at strategic locations |                         |
|                  | refreshed with         | refreshed as needed     |
|                  | disinfectant           |                         |
+------------------+------------------------+-------------------------+
| People at        | Disinfect the spawning | -As per attached        |
| Spawnings        | table and spawning     | iodophor protocol       |
|                  | work area between      |                         |
|                  | stocks and at the end  |                         |
|                  | of the day             |                         |
+------------------+------------------------+-------------------------+
| Research,        | Handle and necropsy    | -Adult morts: use       |
| Hatchery Crew &  | dead fish only in      | concrete pad outside    |
| Pathology        | designated areas       | spawn                   |
| Personnel        |                        |                         |
|                  |                        | area or concrete pad in |
|                  |                        | endemic building at LGH |
|                  |                        |                         |
|                  |                        | -Juvenile morts: store  |
|                  |                        | in freezer in           |
|                  |                        | designated area for     |
|                  |                        | this purpose.           |
+------------------+------------------------+-------------------------+
| PIT taggers      | -PIT tagging           | -if PIT tag needles are |
|                  | supervisors maintain   | re-used disinfect as    |
|                  | and keep footbaths by  | per isopropyl protocol  |
|                  | each door of PIT       | attached                |
|                  | tagging trailer for    |                         |
|                  | use during operations  |                         |
|                  |                        |                         |
|                  | -Assure that PIT       |                         |
|                  | tagging needles are    |                         |
|                  | new or clean and sharp |                         |
|                  |                        |                         |
|                  | -Disinfect in 70%      |                         |
|                  | Isopropyl alcohol      |                         |
|                  |                        |                         |
|                  | -No re-use of PIT tag  |                         |
|                  | needles until air      |                         |
|                  | dried                  |                         |
+------------------+------------------------+-------------------------+
| Lib Truck        | Assure proper          | -As per attached        |
| Operators        | disinfection of tank   | disinfectant            |
|                  | and equipment prior to | application             |
|                  | collection or transfer |                         |
|                  | of fish                | summary                 |
+------------------+------------------------+-------------------------+
| Captive Brood    | See Captive Broodstock | -Appendix 6 Captive     |
| Operations       | AOP                    | Broodstock AOP          |
+------------------+------------------------+-------------------------+

\*Footnote: Within a stock, operations will start with low BKD
segregation groups or groups determined to be of lowest disease risk
proceeding to raceways of higher disease risk. The latest fish health
information should be used to determine the least risky raceway
sequence.

**Appendix C (page 3 of 3): Disinfection and Sanitation Guidelines for
all LSRCP hatcheries**

**Summary of Recommended Disinfectants (Concentration and time) and for
what Application**

+-----------+-------------------+-------------+-----+----------------+
| **Disinfe | **Application**   | **Conc      | **  | **Comment**    |
| ctant\*** |                   | entration** | Tim |                |
|           |                   |             | e** |                |
+-----------+-------------------+-------------+-----+----------------+
| **I       | Nets, gear and    | **100 ppm** | *   | -Equipment     |
| odophor** | equipment,        |             | *10 | should be      |
|           | clipping &        | Note: to    | min | pre-rinsed to  |
|           | tagging van, PIT  | make 100    | **. | remove dirt,   |
|           | tag stations,     | ppm         |     | mucus or other |
|           | large tub         | solution    |     | organic        |
|           | disinfectant      |             |     | material which |
|           | containers,       | mix 6.7 oz  |     | reduces the    |
|           | spawning          | of jug      |     | efficacy of    |
|           | colanders and     | strength    |     | disinfection   |
|           | buckets, lib      | iodophor to |     | and            |
|           | truck, footbaths, |             |     | sanitization   |
|           | floors            | 5 gallons   |     |                |
|           |                   | H~2~O       |     | -Rinse         |
|           | Note: For raceway |             |     | equipment to   |
|           | sanitization\*\*  | or 6.7      |     | remove harmful |
|           | -- thoroughly     | oz.=189ml   |     | residue if     |
|           | clean the unit to |             |     | equipment is   |
|           | remove dirt,      |             |     | going into     |
|           | spray or brush on |             |     | standing water |
|           | 75-100 ppm        |             |     | containing     |
|           | iodophor and let  |             |     | fish or fish   |
|           | this remain for a |             |     | are being      |
|           | minimum of 10     |             |     | placed into    |
|           | minutes. Leave it |             |     | the equipment  |
|           | to dry for a      |             |     | (tank or       |
|           | minimum of 3      |             |     | bucket).       |
|           | days. Allow       |             |     | Remember that  |
|           | iodophor to dry   |             |     | iodine at      |
|           | and break down    |             |     | 1:20,000 is    |
|           | with exposure to  |             |     | harmful to     |
|           | light.            |             |     | fish.          |
|           |                   |             |     |                |
|           | \*\*If the above  |             |     | -Argentyne or  |
|           | recommendation    |             |     | other buffered |
|           | cannot be done    |             |     | iodophors such |
|           | then sanitize     |             |     | as Western     |
|           | raceways by       |             |     | Chemicals "PVP |
|           | thoroughly        |             |     | iodine" would  |
|           | cleaning them and |             |     | be acceptable. |
|           | leaving to dry    |             |     |                |
|           | for a minimum of  |             |     | **Note: if     |
|           | 3 days.           |             |     | DRAW 476 is    |
|           |                   |             |     | used remember  |
|           |                   |             |     | this product   |
|           |                   |             |     | is 1.75%       |
|           |                   |             |     | active iodine  |
|           |                   |             |     | and unbuffered |
|           |                   |             |     | so should not  |
|           |                   |             |     | be used for    |
|           |                   |             |     | water-         |
|           |                   |             |     | hardening      |
|           |                   |             |     | eggs**         |
+-----------+-------------------+-------------+-----+----------------+
|           |                   |             |     |                |
+-----------+-------------------+-------------+-----+----------------+
|           | Water hardening   | 100 ppm     | 15  | This is the    |
|           | eggs              |             | m   | statewide      |
|           |                   |             | inu | general        |
|           |                   |             | tes | practice       |
+-----------+-------------------+-------------+-----+----------------+
|           | Egg transfers -   | 100 ppm     | 10  |                |
|           | disinfection at   |             | m   |                |
|           | receiving station |             | inu |                |
|           |                   |             | tes |                |
+-----------+-------------------+-------------+-----+----------------+
|           |                   |             |     |                |
+-----------+-------------------+-------------+-----+----------------+
| *         | Lib truck tanks   | **10 ppm**  | *   | Organic matter |
| *Chlorine |                   |             | *10 | binds and      |
| or        |                   |             | min | neutralizes    |
| Aqueous   |                   |             | **. |                |
| solution  |                   |             |     |                |
| as sodium |                   |             |     |                |
| hyp       |                   |             |     |                |
| ochlorite |                   |             |     |                |
| (         |                   |             |     |                |
| Household |                   |             |     |                |
| Bleach)** |                   |             |     |                |
+-----------+-------------------+-------------+-----+----------------+

**\*All chemical use will be done in accordance with label use and
reporting requirements**. **Disinfecting and disinfected water must be
disposed of in an approved manner.**

Appendix D. Juvenile Chinook Fish Health Monitoring Plan & Disease
Treatments

+-----+---+---+---+-----+----------------------+--------------------+
| Lo  | B | S | S | Ex  | Protocol             | Comment/Disease    |
| cat | r | p | t | ami |                      | Treatment          |
| ion | o | e | o | nat |                      |                    |
|     | o | c | c | ion |                      |                    |
|     | d | i | k | Ca  |                      |                    |
|     | y | e |   | teg |                      |                    |
|     | e | s |   | ory |                      |                    |
|     | a |   |   |     |                      |                    |
|     | r |   |   |     |                      |                    |
+-----+---+---+---+-----+----------------------+--------------------+
| Ox  | 2 | C | 2 | Pr  | 10 mort/moribund per | One planned        |
| bow | 0 | h | 0 | etr | stock, kidney smears | medicated          |
| Ha  | 0 | S | 0 | ans | on TYE-S agar, R.    | treatment in       |
| tch | 6 |   | W | fer | salmoninarum (BKD),  | July/august 2006   |
| ery |   |   |   |     | Gill & skin wet      |                    |
|     |   |   |   |     | mounts from a        |                    |
|     |   |   |   |     | minimum of 5         |                    |
|     |   |   |   |     | fish/stock           |                    |
+-----+---+---+---+-----+----------------------+--------------------+
| Loo | 2 | C | 2 | M   | -10 mort/moribund    | \*1st Aquamycin    |
| kin | 0 | h | 0 | ont | per stock, kidney    | feeding in         |
| ggl | 0 | S | 0 | hly | smears on TYE-S      | March/April/May    |
| ass | 6 |   | W |     | agar, gill culture   | 2006 at \~500 fpp  |
| Ha  |   |   |   |     | smears if suspect    | for 200F, 201F and |
| tch |   |   | 2 |     | gill disease, R.     | 80F fish.          |
| ery |   |   | 0 |     | salmoninarum (BKD),  |                    |
|     |   |   | 1 |     | Gill & skin wet      | An Aquamycin       |
|     |   |   | W |     | mounts from a        | feeding will be    |
|     |   |   |   |     | combination of       | implemented for    |
|     |   |   | 8 |     | moribund and healthy | all fish after     |
|     |   |   | 0 |     | fish.                | marking in         |
|     |   |   | W |     |                      | July/August        |
|     |   |   |   |     | -tissues (gill/      |                    |
|     |   |   | 2 |     | kidney/spleen) will  | Disease            |
|     |   |   | 9 |     | be assayed for       | outbreaks -        |
|     |   |   |   |     | culturable viruses   | treated on a       |
|     |   |   | \ |     | from a sub-sample of | case-by-case       |
|     |   |   | * |     | fish                 | basis. Therapies   |
|     |   |   | 2 |     |                      | and remedial       |
|     |   |   | 0 |     | -5 grab-sampled fish | measures are based |
|     |   |   | 0 |     | every other month    | on conventional    |
|     |   |   | F |     | and any moribund     | and available      |
|     |   |   |   |     | fish for EIBS (blood | treatments, new    |
|     |   |   | \ |     | smears and           | information, and   |
|     |   |   | * |     | hematocrits).        | innovation. Warm   |
|     |   |   | 2 |     |                      | water temperature  |
|     |   |   | 0 |     |                      | therapy would be   |
|     |   |   | 1 |     |                      | used if EIBS       |
|     |   |   | F |     |                      | became a problem   |
|     |   |   |   |     |                      | on a priority      |
|     |   |   | \ |     |                      | basis determined   |
|     |   |   | * |     |                      | by co-managers.    |
|     |   |   | 8 |     |                      | Formalin           |
|     |   |   | 0 |     |                      | treatments would   |
|     |   |   | F |     |                      | be implemented for |
|     |   |   |   |     |                      | Ichthyobodo        |
|     |   |   |   |     |                      | infestations.      |
|     |   |   |   |     |                      |                    |
|     |   |   |   |     |                      | Fungus - Formalin  |
|     |   |   |   |     |                      | flushes (1 hour)   |
|     |   |   |   |     |                      | are prescribed     |
|     |   |   |   |     |                      | after fin clipping |
|     |   |   |   |     |                      | and coded wire     |
|     |   |   |   |     |                      | tagging for two    |
|     |   |   |   |     |                      | consecutive days.  |
|     |   |   |   |     |                      | Formalin is used   |
|     |   |   |   |     |                      | under a local      |
|     |   |   |   |     |                      | veterinarian       |
|     |   |   |   |     |                      | prescription.      |
|     |   |   |   |     |                      |                    |
|     |   |   |   |     |                      | Coldwater disease- |
|     |   |   |   |     |                      | Oxytetracycline or |
|     |   |   |   |     |                      | Florfenicol maybe  |
|     |   |   |   |     |                      | used.              |
+-----+---+---+---+-----+----------------------+--------------------+
| Loo | 2 | C | 2 | M   | Monthly: As above    | Pre-transfer       |
| kin | 0 | h | 0 | ont |                      | grab-sample        |
| ggl | 0 | S | 0 | hly | Pre-transfer: 60     | numbers may vary   |
| ass | 5 |   | W |     | grab-sampled smolts  | depending on       |
| Ha  |   |   |   | Pre | per stock            | disease history    |
| tch |   |   | 2 | -tr |                      | and number of fish |
| ery |   |   | 0 | ans | -R. salmoninarum by  | for a given brood  |
|     |   |   | 0 | fer | ELISA                | year.              |
|     |   |   | F | &   |                      |                    |
|     |   |   |   |     | -tissues             |                    |
|     |   |   | 2 | Ann | (gill/kidney/spleen) |                    |
|     |   |   | 0 | ual | from 3 fish pools    |                    |
|     |   |   | 1 | Myx | for culturable       |                    |
|     |   |   | W | obo | viruses              |                    |
|     |   |   |   | lus |                      |                    |
|     |   |   | 2 |     | -wet mounts of skin  |                    |
|     |   |   | 0 | c   | & gill tissue from a |                    |
|     |   |   | 1 | ere | minimum of 5 fish    |                    |
|     |   |   | F | bra |                      |                    |
|     |   |   |   | lis | -sub-sample for EIBS |                    |
|     |   |   | 8 | t   |                      |                    |
|     |   |   | 0 | est | -one stock (60 fish) |                    |
|     |   |   | W | ing | for Myxobolus        |                    |
|     |   |   |   |     | cerebralis           |                    |
|     |   |   | 8 |     |                      |                    |
|     |   |   | 0 |     |                      |                    |
|     |   |   | F |     |                      |                    |
|     |   |   |   |     |                      |                    |
|     |   |   | 2 |     |                      |                    |
|     |   |   | 9 |     |                      |                    |
+-----+---+---+---+-----+----------------------+--------------------+
| C   | 2 | C |   | Pr  | -Smolt groups held   | Pre-liberation     |
| hin | 0 | h |   | e-l | at acclimation sites | grab-sample        |
| ook | 0 | S |   | ibe | longer than 3 weeks  | numbers at         |
| ac  | 5 |   |   | rat | will be evaluated    | acclimation sites  |
| cli |   |   |   | ion | with a lesser number | may vary depending |
| mat |   |   |   |     | of "grab-sampled"    | on disease history |
| ion |   |   |   |     | fish as in           | and number of fish |
| IM, |   |   |   |     | pre-transfer         | for a given brood  |
| LR, |   |   |   |     | protocol above.      | year.              |
| CC  |   |   |   |     |                      |                    |
| &   |   |   |   |     | -Mortalities will be |                    |
| UGR |   |   |   |     | examined (as in      |                    |
|     |   |   |   |     | monthly)             |                    |
+-----+---+---+---+-----+----------------------+--------------------+

Appendix E. Adult Chinook Fish Health Monitoring Plan & Disease
Treatments

+-----+---+---+----+-----+----------------+--------------------------+
| Lo  | B | S | S  | Ex  | Protocol       | Comment                  |
| cat | r | p | to | ami |                |                          |
| ion | o | e | ck | nat |                |                          |
|     | o | c |    | ion |                |                          |
|     | d | i |    | Ca  |                |                          |
|     | y | e |    | teg |                |                          |
|     | e | s |    | ory |                |                          |
|     | a |   |    |     |                |                          |
|     | r |   |    |     |                |                          |
+-----+---+---+----+-----+----------------+--------------------------+
| Loo | 2 | C | 20 | Ad  | \*All spawned  | ELISA results will be    |
| kin | 0 | h | 0W | ult | fish will be   | used to implement BKD    |
| ggl | 0 | S |    | Sp  | sampled for    | prevention control       |
| ass | 7 |   | 20 | awn | culturable     | through culling of eggs  |
|     |   |   | 1W | ers | viruses --     | known to be of higher    |
|     |   |   |    |     | individual     | risk.                    |
|     |   |   | 8  | (Br | fish ovarian   |                          |
|     |   |   | 0W | ood | fluid and      | \*Imnaha stock: virus    |
|     |   |   |    | sto | milt, minimum  | sample a minimum of 60   |
|     |   |   | 29 | ck) | of 60 or all   | fish -- a minimum of 24  |
|     |   |   |    |     | fish if \<60   | subsamples per week of   |
|     |   |   |    |     | using          | sex fluids (ovarian      |
|     |   |   |    |     | caeca          | fluid or milt)           |
|     |   |   |    |     | /kidney/spleen |                          |
|     |   |   |    |     | sample pools   |                          |
|     |   |   |    |     | not to exceed  |                          |
|     |   |   |    |     | 5 fish.        |                          |
|     |   |   |    |     |                |                          |
|     |   |   |    |     | \- All females |                          |
|     |   |   |    |     | for BKD by     |                          |
|     |   |   |    |     | ELISA          |                          |
+-----+---+---+----+-----+----------------+--------------------------+
| Loo | 2 | C | 20 | Ad  | All mortality  | Note: additional         |
| kin | 0 | h | 0W | ult | less than 20:  | mortality may be sampled |
| ggl | 0 | S |    | Mor |                |                          |
| ass | 7 |   | 20 | tal | -Kidney        | Lookingglass Creek       |
|     |   |   | 1W | ity | sampled for    | mortalities will be      |
|     |   |   |    |     | BKD by ELISA   | worked up with CTUIR     |
|     |   |   | 8  |     |                | staff to assure data     |
|     |   |   | 0W |     | -systemic      | collection covers all    |
|     |   |   |    |     | bacteria by    | the needed information   |
|     |   |   | 29 |     | culture        |                          |
+-----+---+---+----+-----+----------------+--------------------------+

Disease Treatments and other Drugs for Adult Chinook Broodstock

+-----+---+----+-----+-------+-----------------+---------------------+
| Lo  | B | S  | Tre | Che   | Protocol        | Comment             |
| cat | r | to | atm | mical |                 |                     |
| ion | o | ck | ent | /Drug |                 |                     |
|     | o |    | for |       |                 |                     |
|     | d |    |     |       |                 |                     |
|     |   |    |     |       |                 |                     |
|     | y |    |     |       |                 |                     |
|     | e |    |     |       |                 |                     |
|     | a |    |     |       |                 |                     |
|     | r |    |     |       |                 |                     |
+-----+---+----+-----+-------+-----------------+---------------------+
| Loo | 2 | 20 | Fun | For   | Formalin        | If formalin cannot  |
| kin | 0 | 0W | gus | malin | administered a  | be used then use    |
| ggl | 0 |    | C   |       | minimum of 3    | hydrogen peroxide   |
| ass | 7 | 20 | ont | Hyd   | days per week   | (second choice)     |
|     |   | 1W | rol | rogen | at 167 ppm for  |                     |
|     |   |    |     | Per   | 1 hr.           | Continue treatments |
|     |   | 8  |     | oxide |                 | throughout the      |
|     |   | 0W |     |       | (Veterinary     | entire spawning     |
|     |   |    |     |       | prescription)   | season.             |
|     |   | 29 |     |       |                 |                     |
|     |   | 00 |     |       | Hydrogen        |                     |
|     |   |    |     |       | peroxide 3 days |                     |
|     |   |    |     |       | per week at 100 |                     |
|     |   |    |     |       | ppm             |                     |
+-----+---+----+-----+-------+-----------------+---------------------+
| L   | 2 | 20 | BKD | Er    | Injection 20    | Erythromycin 100 -- |
| ook | 0 | 0W |     | ythro | mg/kg           | new product now     |
| ing | 0 |    | Fur | mycin |                 | available           |
| gla | 7 | 20 | unc |       | (Veterinary     |                     |
| ss, |   | 1W | ulo | Oxyte | Prescription)   | New charts will be  |
|     |   |    | sis | tracy |                 | provided, if        |
| Cat |   | 8  |     | cline | Injection 10    | needed.             |
| her |   | 0W |     |       | mg/kg           |                     |
| ine |   |    |     |       |                 |                     |
| Cre |   | 2  |     |       | (Veterinary     |                     |
| ek, |   | 9W |     |       | Prescription)   |                     |
| Up  |   |    |     |       |                 |                     |
| per |   |    |     |       |                 |                     |
| Gra |   |    |     |       |                 |                     |
| nde |   |    |     |       |                 |                     |
| Ro  |   |    |     |       |                 |                     |
| nde |   |    |     |       |                 |                     |
| and |   |    |     |       |                 |                     |
| L   |   |    |     |       |                 |                     |
| ost |   |    |     |       |                 |                     |
| ine |   |    |     |       |                 |                     |
| Ri  |   |    |     |       |                 |                     |
| ver |   |    |     |       |                 |                     |
| we  |   |    |     |       |                 |                     |
| irs |   |    |     |       |                 |                     |
+-----+---+----+-----+-------+-----------------+---------------------+
| Loo | 2 | 20 | A   | A     | INAD            | Send reports to     |
| kin | 0 | 0W | nes | qui-S |                 | Monitor by December |
| ggl | 0 |    | the |       |                 | 1st 2007.           |
| ass | 7 | 20 | tic |       |                 |                     |
|     |   | 1W |     |       |                 |                     |
|     |   |    |     |       |                 |                     |
|     |   | 8  |     |       |                 |                     |
|     |   | 0W |     |       |                 |                     |
|     |   |    |     |       |                 |                     |
|     |   | 2  |     |       |                 |                     |
|     |   | 9W |     |       |                 |                     |
+-----+---+----+-----+-------+-----------------+---------------------+

**~Appendix\ F.\ Ponding\ plan\ for\ 2007\ at\ Lookingglass\ Hatchery.~**

<table>
<colgroup>
<col style="width: 13%" />
<col style="width: 9%" />
<col style="width: 10%" />
<col style="width: 6%" />
<col style="width: 6%" />
<col style="width: 5%" />
<col style="width: 12%" />
<col style="width: 8%" />
<col style="width: 5%" />
<col style="width: 6%" />
<col style="width: 8%" />
<col style="width: 6%" />
</colgroup>
<tbody>
<tr class="odd">
<td>Group</td>
<td>Strategy Treatment</td>
<td>BKD</td>
<td>Eyed Eggs (Dec 06)</td>
<td><p>Initial</p>
<p>Ponding</p>
<p>(fry)</p></td>
<td><p>Initial</p>
<p>Pond #</p></td>
<td></td>
<td><p>Smolts</p>
<p>marked</p></td>
<td><p>Final</p>
<p>Pond #</p></td>
<td>Smolt Numbers</td>
<td>Mark</td>
<td>Release Site</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>From rcy 3 into 1</td>
<td>60,900</td>
<td>1</td>
<td><strong>60,900</strong></td>
<td>AdCWT</td>
<td>CC</td>
</tr>
<tr class="odd">
<td>Catherine Creek</td>
<td>Conventional</td>
<td>Low</td>
<td>121,800</td>
<td>121,800</td>
<td>3</td>
<td>From rcy 3 into 2</td>
<td>60,900</td>
<td>2</td>
<td><strong>60,900</strong></td>
<td>Ad only</td>
<td>CC</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td>Catherine Creek</td>
<td>CBS</td>
<td>Low/mod-low</td>
<td>29,000</td>
<td>29,000</td>
<td>4</td>
<td>From rcy 4 into 3</td>
<td>29,000</td>
<td>3</td>
<td><strong>29,000</strong></td>
<td>AdCWT</td>
<td>Look</td>
</tr>
<tr class="even">
<td>Catherine Creek</td>
<td>CBS</td>
<td>Mod-hi</td>
<td>21,000</td>
<td>21,000</td>
<td>5</td>
<td>From rcy 5 into 4</td>
<td>21,000</td>
<td>4</td>
<td><strong>21,000</strong></td>
<td>AdCWT</td>
<td>Look</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td>U. Grande Ronde</td>
<td>Conventional</td>
<td>Low</td>
<td>256,000</td>
<td>85,333</td>
<td>7</td>
<td>From rcy 7 into 5</td>
<td>64,000</td>
<td>5</td>
<td><strong>64,000</strong></td>
<td>AdCWT</td>
<td>UGR</td>
</tr>
<tr class="odd">
<td colspan="3" rowspan="3"><strong>Note: UGR marking is under dispute
and may change through US v Oregon resolution</strong></td>
<td></td>
<td>85,333</td>
<td>8</td>
<td>From rcy 7&amp;8 into 6</td>
<td>64,000</td>
<td>6</td>
<td><strong>64,000</strong></td>
<td>Ad</td>
<td>UGR</td>
</tr>
<tr class="even">
<td></td>
<td>85,333</td>
<td>9</td>
<td>From rcy 8&amp;9 into 7</td>
<td>64,000</td>
<td>7</td>
<td><strong>64,000</strong></td>
<td>Ad</td>
<td>UGR</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td>From rcy 9 into 8</td>
<td>64,000</td>
<td>8</td>
<td><strong>64,000</strong></td>
<td>Ad</td>
<td>UGR</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td>Lostine River</td>
<td>Conventional</td>
<td>Low</td>
<td>206,300</td>
<td>101,400</td>
<td>11</td>
<td>From rcy 11 to 9&amp;10</td>
<td>67,600</td>
<td>9</td>
<td><strong>66,000</strong></td>
<td>AdCWT</td>
<td>LR</td>
</tr>
<tr class="even">
<td>Lostine River</td>
<td>Conventional from Oxbow</td>
<td>Low</td>
<td></td>
<td>101,400</td>
<td>12</td>
<td>From 11&amp;12 to 10</td>
<td>67,600</td>
<td>10</td>
<td><strong>66,000</strong></td>
<td>AdCWT</td>
<td>LR</td>
</tr>
<tr class="odd">
<td>Lostine River</td>
<td></td>
<td>Low</td>
<td></td>
<td></td>
<td></td>
<td>From rcy 12 io 11</td>
<td>67,600</td>
<td>11</td>
<td><strong>66,000</strong></td>
<td>AdCWT</td>
<td>LR</td>
</tr>
<tr class="even">
<td>Lostine River</td>
<td>CBS</td>
<td>Low; Mod/low</td>
<td>14,000</td>
<td>14,000</td>
<td>13</td>
<td>From rcy 13 into 12</td>
<td>14,000</td>
<td>12</td>
<td><strong>14,000</strong></td>
<td>Ad only</td>
<td>LR</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td>Imnaha River</td>
<td>Conventional</td>
<td>Low</td>
<td>366,000</td>
<td>91,200</td>
<td>15</td>
<td>From 15 into 13&amp;14</td>
<td>60,800</td>
<td>13</td>
<td><strong>60,800</strong></td>
<td>AdCWT</td>
<td>IM</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td>91,200</td>
<td>16</td>
<td>From 16 into 14-15</td>
<td>60,800</td>
<td>14</td>
<td><strong>60,800</strong></td>
<td>AdCWT</td>
<td>IM</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td>91,200</td>
<td>17</td>
<td>From 17 into 16-17</td>
<td>60,800</td>
<td>15</td>
<td><strong>60,800</strong></td>
<td>AdCWT</td>
<td>IM</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td>91,200</td>
<td>18</td>
<td>From 18 into 17&amp;18</td>
<td>60,800</td>
<td>16</td>
<td><strong>60,800</strong></td>
<td>Ad only</td>
<td>IM</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>60,800</td>
<td>17</td>
<td><strong>60,800</strong></td>
<td>Ad only</td>
<td>IM</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>60,800</td>
<td>18</td>
<td><strong>60,800</strong></td>
<td>Ad only</td>
<td>IM</td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
</tbody>
</table>

## 

## Appendix G. Snake River Germplasm Repository Cryopreserved Semen Request Form

# Snake River Germplasm Repository Committee

Bill Young

P.O. Box 1942, 125 South Mission St

McCall, ID 83638

Phone: (208) 634-5290

Fax: (208) 634-4097

**Snake River Germplasm Repository Cryopreserved Semen Request Form**

Name:
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_Affiliation:
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Phone number: (\_\_\_\_\_\_)\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_Address:
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Date of request: \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_Date
need by: \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Species/stock requested: \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_Hatchery or
wild/natural: \_\_\_\_\_\_\_\_\_\_

Number of individuals: \_\_\_\_\_\_\_Number of straws needed:
\_\_\_\_\_0.5ml \_\_\_\_\_5.0ml

Reason for request (clearly demonstrate need or type of hatchery
program): \_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Fertilization experience using cryopreserved semen:
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Name, address, and phone number of person samples should be delivered
to: \_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Please use additional pages as necessary.

The salmon managers of the Snake River Basin are concerned with how
cryopreserved samples are being used and retain the right to refuse
samples for inappropriate use of the threatened salmonid species
gametes. The Nez Perce Tribe can arrange to deliver and assist in the
fertilization of eggs. Please call Bill Young at the McCall Field Office
(address above) to coordinate transfer. The Nez Perce Tribe also may
request data on the performance of the semen (percent of eggs
fertilized, post-thaw sperm motility, etc.).

### Signature: \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_Date: \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**Appendix H. Table Summarizing Adult Collection Fish Health Injection
Protocols for Grande Ronde and Imnaha chinook**

> Plan A: Operations under the assumption that weir is in place for each
> respective system by July 1, 2007

+-------+---------+-----------+--------+-------+---------------------+
|       | Injec   |           |        |       |                     |
|       | tion^a^ |           |        |       |                     |
|       | (Yes or |           |        |       |                     |
|       | No)     |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
| Ar    | Eryt    | Oxytet    | When   | Which | Comment             |
| rival | hro-100 | racycline |        | Fish  |                     |
| Dates | \@      | @ 10mg/Kg |        |       |                     |
|       | 20mg/Kg |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
|       |         |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
| Up to | Yes     | Yes       | Upon   | brood | Only fish kept for  |
|       |         |           | Coll   | stock | broodstock          |
| July  |         |           | ection |       |                     |
| 14    |         |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
|       |         |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
| Jul   | Not at  | Not at    | During | brood | Only fish kept for  |
| 15    | col     | c         | s      | stock | broodstock          |
| -July | lection | ollection | orting |       |                     |
| 31    |         |           | at     |       |                     |
|       |         |           | Lookin |       |                     |
|       |         |           | gglass |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
|       |         |           |        |       |                     |
+-------+---------+-----------+--------+-------+---------------------+
| After | Yes     | Yes       | Upon   | brood | At Lookingglass on  |
| July  |         |           | Coll   | stock | or about August 1.  |
| 31    |         |           | ection |       |                     |
|       |         |           |        |       | 2^nd^ injection for |
|       |         |           |        |       | arrivals before     |
|       |         |           |        |       | July 14             |
|       |         |           |        |       |                     |
|       |         |           |        |       | 1^st^ Injection for |
|       |         |           |        |       | arrivals after July |
|       |         |           |        |       | 14                  |
|       |         |           |        |       |                     |
|       |         |           |        |       | Note: more than one |
|       |         |           |        |       | day may be needed   |
|       |         |           |        |       | for all injections  |
+-------+---------+-----------+--------+-------+---------------------+

Plan B: Assumes weirs were unable to be placed in river by July 1, 2007

  --------- ------------- ----------------- ------------ -------- ----------------------
            Injection                                             
            (Yes or No)                                           

  Arrival   Erythro-100   Oxytetracycline @ When         Which    Comment
  Dates     \@20mg/Kg     10mg/Kg                        Fish     

                                                                  

  Entire    Yes           Yes               Upon         All      No planned
  Run                                       Collection   unripe   re-injection under
  Period                                                 fish     this scenario
  --------- ------------- ----------------- ------------ -------- ----------------------

Injection Route Plan for Antibiotic Injections 2007

  -------------- -------------- ------------------ ----------------- ---------------
  Stock/Group    Erythromycin   Erythromycin       Oxytetracycline   Comment
                 first          second injection   all injections -- 
                 injection      (or first          first & second    
                                injection for late                   
                                arrivals)                            

  Imnaha &       IP             IP                 IP                
  Lostine                                                            

  CC & GR        DS             DS                 IP                
  Conventional                                                       

  LG-CK          IP             IP                 IP                Swim-ins or
  production                                                         fish trucked
  fish                                                               for broodstock,
                                                                     If released
                                                                     need 21 detox
                                                                     period or no
                                                                     injection
  -------------- -------------- ------------------ ----------------- ---------------

**Appendix I. Attachment for Imnaha and Grande Ronde Broodstock
Antibiotic Injection Protocols**

**Modified by Sam Onjukka for 2007**

Oregon Department of Fish and Wildlife

La Grande Fish Health Services Laboratory

> The La Grande Fish Health Services Laboratory provides fish health
> support services for these programs. The required prescriptions for
> the antibiotic treatments will be obtained from state veterinarian Dr.
> Collin Gillin via ODFW Fish Health staff. These prescriptions and
> protocols apply to the injection of the antibiotics erythromycin and
> oxytetracycline. Note: Erythromycin-100 is available from Bimeda.
> Contact person: David A Hance (1-877-672-6762). Note: The new product
> has 100 mg erythromycin/ml (old product had 200 mg/ml) thus you will
> have to double the volume to achieve the prescribed 20 mg/kg. A 3 cc
> syringe will probably be necessary in most cases.

##### At collection sites

Injection schedule: (Follow Appendix E and injection route plan table at
bottom of page)

Methods:

1\) Use erythromycin or gallimycin 100 and oxytetracycline 100 (200
mg/mL).

2\) For Intraperitoneal (IP) injections use 1 inch 20 gauge needle. For
Dorsal Sinus (DS) use a 1inch or 1 ½ inch 20 Gauge needle. Though both
needle lengths can be used, stick with one needle length for DS
injections so the technique for injecting the dorsal sinus is consistent
and accurate. More leakage will occur if you are not injecting the
erythromycin into the sinus.

Use a separate syringe and needle for each antibiotic and fish. See
points below to minimize egg mass loss with IP injection of erythromycin

[Dosage:]{.underline}

Inject a volume of each antibiotic according to the fish specific length
or weight corresponding to the 10 mg/Kg OXY-200 chart for
Oxytetracycline and the 20 mg/Kg Ery-100 chart for Erythromycin. Note:
injections may also be given based on bracketed size ranges (as per
information provided in 2006). Fish Health Services will provide
specific charts and information in May 2007, if needed..

##### 

##### At Lookingglass Hatchery

On or about August 1, inject all adults on hand per the protocol
(Appendix H). This will be a second injection for fish that arrived
before July 14 and it will be a first injection for fish that arrived
between July 15 and August 1.

Do not inject ripe fish!

Dispose of all needles in sharps containers and all will be properly
disposed of at Grande Ronde Recycling at the end of the season.

[To minimize egg mass loss due to IP injections:]{.underline}

Insert needle no deeper than necessary. Place the bevel of needle
towards the body wall of fish so drug goes out against the wall rather
than the eggs or body cavity. Keep the head slightly down to help shift
away the egg skein from the injection location.

Appendix J. Catherine Creek, Lostine, and Upper Grande Ronde fish
culture production metrics summarized for Grande Ronde sub-basin Chinook
stocks.

Catherine Creek spring/summer Chinook salmon spawning data for the
2001-06

+----+-------+-------+-----+-------+-------+---------+-------+--------+
| B  | M     | Unm   | \%  | Spa   | Av    | Egg     | Fry   | Smolt  |
| ro | arked | arked | Un- | wning | erage | Take    | P     | re     |
| od | Fe    | Fe    | mar | Ratio | Fecu  |         | onded | leases |
| Ye | males | males | ked | F/M   | ndity |         |       |        |
| ar | Sp    | Sp    |     |       |       |         |       |        |
|    | awned | awned |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 12    | 1   | 1     | 3,651 | 43,813  | 2     | 24,392 |
| 01 |       |       | 00% | .71:1 |       |         | 6,426 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 20    | 1   | 1     | 4,096 | 81,926  | 7     | 70,959 |
| 02 |       |       | 00% | .18:1 |       |         | 1,750 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| ** | 0     | 28    | 1   | 1     | **4,  | **12    | *     | **120  |
| 20 |       |       | 00% | .47:1 | 639** | 9,888** | *123, | ,753** |
| 03 |       |       |     |       |       |         | 394** |        |
| \* |       |       |     |       |       |         |       |        |
| ** |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 9     | 1   | 1     | 2,912 | 26,204  | 2     | 23,216 |
| 04 |       |       | 00% | .50:1 |       |         | 4,465 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 9     | 8     | 30  | 1     | 3,149 | 53,533  | 4     | **49   |
| 05 |       |       | .0% | .42:1 |       |         | 9,222 | ,863** |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 28    | 8     | 22  | 1     | 3,642 | 131,139 | *     |        |
| 06 |       |       | .2% | .24:1 |       |         | *121, |        |
|    |       |       |     |       |       |         | 868** |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
|    | *     | *     | **6 |       | **3,  | **46    |       | **289  |
|    | *37** | *85** | 9.7 |       | 824** | 6,503** |       | ,183** |
|    |       |       | %** |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| \  |       |       |     |       |       |         |       |        |
| *I |       |       |     |       |       |         |       |        |
| nv |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| ry |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| or |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| ct |       |       |     |       |       |         |       |        |
| io |       |       |     |       |       |         |       |        |
| n; |       |       |     |       |       |         |       |        |
| In |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 4, |       |       |     |       |       |         |       |        |
| eg |       |       |     |       |       |         |       |        |
| gs |       |       |     |       |       |         |       |        |
| ha |       |       |     |       |       |         |       |        |
| ve |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| el |       |       |     |       |       |         |       |        |
| ec |       |       |     |       |       |         |       |        |
| tr |       |       |     |       |       |         |       |        |
| on |       |       |     |       |       |         |       |        |
| ic |       |       |     |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
| ly |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| ou |       |       |     |       |       |         |       |        |
| nt |       |       |     |       |       |         |       |        |
| ed |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| N  |       |       |     |       |       |         |       |        |
| um |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| rs |       |       |     |       |       |         |       |        |
| in |       |       |     |       |       |         |       |        |
| bl |       |       |     |       |       |         |       |        |
| ue |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| ur |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| nt |       |       |     |       |       |         |       |        |
| i  |       |       |     |       |       |         |       |        |
| nv |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| ry |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 1- |       |       |     |       |       |         |       |        |
| 05 |       |       |     |       |       |         |       |        |
| br |       |       |     |       |       |         |       |        |
| oo |       |       |     |       |       |         |       |        |
| d, |       |       |     |       |       |         |       |        |
| es |       |       |     |       |       |         |       |        |
| ti |       |       |     |       |       |         |       |        |
| ma |       |       |     |       |       |         |       |        |
| te |       |       |     |       |       |         |       |        |
| su |       |       |     |       |       |         |       |        |
| rv |       |       |     |       |       |         |       |        |
| iv |       |       |     |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
| fr |       |       |     |       |       |         |       |        |
| om |       |       |     |       |       |         |       |        |
| g  |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| e  |       |       |     |       |       |         |       |        |
| gg |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| s  |       |       |     |       |       |         |       |        |
| mo |       |       |     |       |       |         |       |        |
| lt |       |       |     |       |       |         |       |        |
| at |       |       |     |       |       |         |       |        |
| 8  |       |       |     |       |       |         |       |        |
| 6. |       |       |     |       |       |         |       |        |
| 2% |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+

Upper Grande Ronde River spring/summer Chinook salmon spawning data
2001-06

+----+-------+-------+-----+-------+-------+---------+-------+--------+
| B  | M     | Unm   | \%  | Spa   | Av    | Egg     | Fry   | Smolt  |
| ro | arked | arked | Un- | wning | erage | Take    | P     | re     |
| od | Fe    | Fe    | mar | Ratio | Fecu  |         | onded | leases |
| Ye | males | males | ked | F/M   | ndity |         |       |        |
| ar | Sp    | Sp    |     |       |       |         |       |        |
|    | awned | awned |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 8     | 1   | 1     | 4,420 | 35,360  | **    | 26,923 |
| 01 |       |       | 00% | .00:1 |       |         | \*25, |        |
|    |       |       |     |       |       |         | 339** |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 25    | 1   | 1     | 3,454 | 86,355  | 7     | 70,088 |
| 02 |       |       | 00% | .09:1 |       |         | 0,250 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 23    | 1   | 1     | 5,249 | 120,733 | 10    | 1      |
| 03 |       |       | 00% | .10:1 |       |         | 5,374 | 04,347 |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 7     | 1   | 1     | 2,979 | 20,850  | 1     | 18,901 |
| 04 |       |       | 00% | .00:1 |       |         | 9,057 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 37    | 3     | 9%  | 1     | 3,877 | 155,080 | 11    | **119  |
| 05 |       |       |     | .54:1 |       |         | 9,963 | ,000** |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 71    | 13    | 15% | 1     | 3,539 | 297,244 | *     |        |
| 06 |       |       |     | .45:1 |       |         | *269, |        |
|    |       |       |     |       |       |         | 439** |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| #  | **    | *     | **4 |       | **3,  | **71    |       | **339  |
|  T | 108** | *79** | 2.2 |       | 827** | 5,622** |       | ,259** |
| ot |       |       | %** |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| \  |       |       |     |       |       |         |       |        |
| *I |       |       |     |       |       |         |       |        |
| nv |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| ry |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| or |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| ct |       |       |     |       |       |         |       |        |
| io |       |       |     |       |       |         |       |        |
| n; |       |       |     |       |       |         |       |        |
| In |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 4, |       |       |     |       |       |         |       |        |
| eg |       |       |     |       |       |         |       |        |
| gs |       |       |     |       |       |         |       |        |
| ha |       |       |     |       |       |         |       |        |
| ve |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| el |       |       |     |       |       |         |       |        |
| ec |       |       |     |       |       |         |       |        |
| tr |       |       |     |       |       |         |       |        |
| on |       |       |     |       |       |         |       |        |
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| ou |       |       |     |       |       |         |       |        |
| nt |       |       |     |       |       |         |       |        |
| ed |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| N  |       |       |     |       |       |         |       |        |
| um |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| rs |       |       |     |       |       |         |       |        |
| in |       |       |     |       |       |         |       |        |
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| ue |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
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| en |       |       |     |       |       |         |       |        |
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|    |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 1- |       |       |     |       |       |         |       |        |
| 05 |       |       |     |       |       |         |       |        |
| br |       |       |     |       |       |         |       |        |
| oo |       |       |     |       |       |         |       |        |
| d, |       |       |     |       |       |         |       |        |
| es |       |       |     |       |       |         |       |        |
| ti |       |       |     |       |       |         |       |        |
| ma |       |       |     |       |       |         |       |        |
| te |       |       |     |       |       |         |       |        |
| su |       |       |     |       |       |         |       |        |
| rv |       |       |     |       |       |         |       |        |
| iv |       |       |     |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
| fr |       |       |     |       |       |         |       |        |
| om |       |       |     |       |       |         |       |        |
| g  |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| e  |       |       |     |       |       |         |       |        |
| gg |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| s  |       |       |     |       |       |         |       |        |
| mo |       |       |     |       |       |         |       |        |
| lt |       |       |     |       |       |         |       |        |
| at |       |       |     |       |       |         |       |        |
| 81 |       |       |     |       |       |         |       |        |
| .1 |       |       |     |       |       |         |       |        |
| %. |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+

Lostine River spring/summer Chinook salmon spawning data, 1997-2006

+----+-------+-------+-----+-------+-------+---------+-------+--------+
| B  | M     | Unm   | \%  | Spa   | Av    | Egg     | Fry   | Smolt  |
| ro | arked | arked | Un- | wning | erage | Take    | P     | re     |
| od | Fe    | Fe    | mar | Ratio | Fecu  |         | onded | leases |
| Ye | males | males | ked | F/M   | ndity |         |       | (1,    |
| ar | Sp    | Sp    |     |       |       |         |       | 000's) |
|    | awned | awned |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 19 | 0     | 4     | 1   | 0     | 4,496 | 17,000  | 1     | 11,871 |
| 97 |       |       | 00% | .92:1 |       |         | 2,000 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 19 | 0     | 0     | 0   | 0     | 0     | 0       | 0     | 0      |
| 98 |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 19 | 0     | 0     | 0   | 0     | 0     | 0       | 0     | 0      |
| 99 |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 8     | 1   | 0     | 4,329 | 34,630  | 3     | 31,490 |
| 00 |       |       | 00% | .66:1 |       |         | 2,000 |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 11    | 25    | 69% | 1     | 4,463 | \*      | 10    | 1      |
| 01 |       |       |     | .06:1 |       | 160,680 | 5,000 | 01,012 |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 1     | 27    | 96% | 1     | 4,766 | 133,444 | 13    | 1      |
| 02 |       |       |     | .03:1 |       |         | 0,000 | 16,370 |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 0     | 21    | 1   | 1     | 5,078 | 106,646 | 10    | 1      |
| 03 |       |       | 00% | .31:1 |       |         | 3,000 | 02,557 |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 29    | 22    | 43% | 1     | 4,351 | 221,888 | 20    | 1      |
| 04 |       |       |     | .30:1 |       |         | 6,421 | 99,716 |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 39    | 17    | 30% | 1     | 4,182 | 234,192 | 20    | **205  |
| 05 |       |       |     | .37:1 |       |         | 7,291 | ,000** |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| 20 | 45    | 12    | 21% | 1     | 4,393 | 241,715 | *     |        |
| 06 |       |       |     | .26:1 |       |         | *206, |        |
|    |       |       |     |       |       |         | 313** |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
|    | **    | **    | **5 |       | **4,  | **1,15  |       | **768  |
|    | 125** | 136** | 2.1 |       | 370** | 0,195** |       | ,016** |
|    |       |       | %** |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+
| \  |       |       |     |       |       |         |       |        |
| *I |       |       |     |       |       |         |       |        |
| nv |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| ry |       |       |     |       |       |         |       |        |
| co |       |       |     |       |       |         |       |        |
| rr |       |       |     |       |       |         |       |        |
| ec |       |       |     |       |       |         |       |        |
| ti |       |       |     |       |       |         |       |        |
| on |       |       |     |       |       |         |       |        |
| d  |       |       |     |       |       |         |       |        |
| ue |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| l  |       |       |     |       |       |         |       |        |
| ar |       |       |     |       |       |         |       |        |
| ge |       |       |     |       |       |         |       |        |
| lo |       |       |     |       |       |         |       |        |
| ss |       |       |     |       |       |         |       |        |
| es |       |       |     |       |       |         |       |        |
| wi |       |       |     |       |       |         |       |        |
| th |       |       |     |       |       |         |       |        |
| e  |       |       |     |       |       |         |       |        |
| gg |       |       |     |       |       |         |       |        |
| s  |       |       |     |       |       |         |       |        |
| hi |       |       |     |       |       |         |       |        |
| pm |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| t; |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| In |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 4, |       |       |     |       |       |         |       |        |
| eg |       |       |     |       |       |         |       |        |
| gs |       |       |     |       |       |         |       |        |
| ha |       |       |     |       |       |         |       |        |
| ve |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| el |       |       |     |       |       |         |       |        |
| ec |       |       |     |       |       |         |       |        |
| tr |       |       |     |       |       |         |       |        |
| on |       |       |     |       |       |         |       |        |
| ic |       |       |     |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
| ly |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| ou |       |       |     |       |       |         |       |        |
| nt |       |       |     |       |       |         |       |        |
| ed |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| N  |       |       |     |       |       |         |       |        |
| um |       |       |     |       |       |         |       |        |
| be |       |       |     |       |       |         |       |        |
| rs |       |       |     |       |       |         |       |        |
| in |       |       |     |       |       |         |       |        |
| bl |       |       |     |       |       |         |       |        |
| ue |       |       |     |       |       |         |       |        |
| c  |       |       |     |       |       |         |       |        |
| ur |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| nt |       |       |     |       |       |         |       |        |
| i  |       |       |     |       |       |         |       |        |
| nv |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| ry |       |       |     |       |       |         |       |        |
|    |       |       |     |       |       |         |       |        |
| 2  |       |       |     |       |       |         |       |        |
| 00 |       |       |     |       |       |         |       |        |
| 1- |       |       |     |       |       |         |       |        |
| 05 |       |       |     |       |       |         |       |        |
| br |       |       |     |       |       |         |       |        |
| oo |       |       |     |       |       |         |       |        |
| d, |       |       |     |       |       |         |       |        |
| es |       |       |     |       |       |         |       |        |
| ti |       |       |     |       |       |         |       |        |
| ma |       |       |     |       |       |         |       |        |
| te |       |       |     |       |       |         |       |        |
| su |       |       |     |       |       |         |       |        |
| rv |       |       |     |       |       |         |       |        |
| iv |       |       |     |       |       |         |       |        |
| al |       |       |     |       |       |         |       |        |
| fr |       |       |     |       |       |         |       |        |
| om |       |       |     |       |       |         |       |        |
| g  |       |       |     |       |       |         |       |        |
| re |       |       |     |       |       |         |       |        |
| en |       |       |     |       |       |         |       |        |
| e  |       |       |     |       |       |         |       |        |
| gg |       |       |     |       |       |         |       |        |
| to |       |       |     |       |       |         |       |        |
| s  |       |       |     |       |       |         |       |        |
| mo |       |       |     |       |       |         |       |        |
| lt |       |       |     |       |       |         |       |        |
| at |       |       |     |       |       |         |       |        |
| 8  |       |       |     |       |       |         |       |        |
| 4. |       |       |     |       |       |         |       |        |
| 5% |       |       |     |       |       |         |       |        |
+----+-------+-------+-----+-------+-------+---------+-------+--------+

**Appendix K. Preliminary CTUIR data from Catherine Creek releases
between 1998 and 2007 summarized by ODFW.**

+-----+-----+----------+-------+-------+------+-----+------+--------+
| #   | **  |          |       |       |      |     | *    | *      |
|  Br | Rel |          |       |       |      |     | *Tot | *SAR** |
| ood | eas |          |       |       |      |     | al** |        |
|     | e** |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| **  | **  | *        | ### N | **R   |      |     | **   | **Per  |
| Yea | Yea | *Release | umber | eturn |      |     | Retu | cent** |
| r** | r** | Type**   |       | Ye    |      |     | rn** |        |
|     |     |          |       | ars** |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2001 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 2]{. | 003 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 2   | Conv     | 0     | \-    | \-   | \-  | \-   | \-     |
| 998 | 000 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 3     | 157   | 205  | 57  | 419  | 1.10   |
|     |     |          | 8,149 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 34    | 46    | 190  | 192 | 428  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2002 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 3]{. | 004 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 2   | Conv     | 0     | \-    | \-   | \-  | \-   | \-     |
| 999 | 001 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 13    | 17    | 194  | 19  | 230  | 0.168  |
|     |     |          | 6,833 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 40    | 19    | 71   | 9   | 99   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2003 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 4]{. | 005 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 0     | \-    | \-   | \-  | \-   | \-     |
| 000 | 002 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 18    | 73    | 570  | 24  | 667  | 0.3699 |
|     |     |          | 0,343 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 34    | 6     | 77   | 7   | 90   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2004 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 5]{. | 006 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 2     | 22    | 27   | 5   | 54   | 0.2214 |
| 001 | 003 | entional | 4,392 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 10    | 39    | 89   | 2   | 130  | 0.1235 |
|     |     |          | 5,292 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 133   | 5     | 49   | 7   | 61   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2005 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 6]{. | 007 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 7     | 10    | 69   |     | 79   | *0     |
| 002 | 004 | entional | 0,071 |       |      |     |      | .1113* |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 9     | 19    | 138  |     | 157  | *0     |
|     |     |          | 1,791 |       |      |     |      | .1710* |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 158   | 4     | 114  |     | 118  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2006 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 7]{. | 008 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 12    | 7     |      |     | 7    |        |
| 003 | 005 | entional | 0,753 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 6     | 0     |      |     | 0    |        |
|     |     |          | 8,827 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 167   | 12    |      |     | 12   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2007 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 8]{. | 009 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 2     |       |      |     |      |        |
| 004 | 006 | entional | 3,216 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 4     |       |      |     |      |        |
|     |     |          | 5,604 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 96    |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2008 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 9]{. | 010 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | *49   |       |      |     |      |        |
| 005 | 007 | entional | ,783* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | *21   |       |      |     |      |        |
|     |     |          | ,647* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | *74*  |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     | #    |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   | #    |        |
|     |     |          |       | [2009 | [201 | *[2 |      |        |
|     |     |          |       | ]{.un | 0]{. | 011 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | *121  |       |      |     |      |        |
| 006 | 008 | entional | ,800* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | *0*   |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 117   |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+

**Appendix L. Preliminary CTUIR data from Upper Grande Ronde releases
between 1998 and 2007 summarized by ODFW.**

+-----+-----+----------+-------+-------+------+-----+------+--------+
| #   | **  |          |       |       |      |     | *    | *      |
|  Br | Rel |          |       |       |      |     | *Tot | *SAR** |
| ood | eas |          |       |       |      |     | al** |        |
|     | e** |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| **  | **  | *        | ### N | **R   |      |     | **   | **Per  |
| Yea | Yea | *Release | umber | eturn |      |     | Retu | cent** |
| r** | r** | Type**   |       | Ye    |      |     | rn** |        |
|     |     |          |       | ars** |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2001 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 2]{. | 003 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 2   | Conv     | 0     |       |      |     |      |        |
| 998 | 000 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 1,508 | 0     | 3    | 1   | 4    | 0.2653 |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 42    | 0     | 83   | 229 | 312  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2002 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 3]{. | 004 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 2   | Conv     | 0     |       |      |     |      |        |
| 999 | 001 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 2,560 | 0     | 8    | 6   | 14   | 0.5469 |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natural- | 0     | 1     | 6    | 0   | 7    |        |
|     |     | Redds^1^ |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2003 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 4]{. | 005 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 0     |       |      |     |      |        |
| 000 | 002 | entional |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captiv   | 22    | 60    | 545  | 26  | 631  | 0.2763 |
|     |     | e^2,\ 3^ | 8,385 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 20    | 3     | 43   | 9   | 55   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2004 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 5]{. | 006 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 2     | 12    | 92   | 0   | 104  | 0.3863 |
| 001 | 003 | entional | 6,923 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Ca       | 24    | 73    | 276  | 7   | 356  | 0.1465 |
|     |     | ptive^4^ | 2,913 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 15    | 6     | 12   | 2   | 20   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2005 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 6]{. | 007 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 6     | 9     | 159  |     | 168  | *0     |
| 002 | 004 | entional | 9,856 |       |      |     |      | .2405* |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 7     | 0     | 1    |     | 1    | *0     |
|     |     |          | 5,063 |       |      |     |      | .0013* |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 23    | 0     | 52   |     | 52   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2006 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 7]{. | 008 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Convent  | 10    | 2     |      |     | 2    |        |
| 003 | 005 | ional^5^ | 4,350 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 1,019 | 0     |      |     | 0    |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 40    | 4     |      |     | 4    |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2007 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 8]{. | 009 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | 1     |       |      |     |      |        |
| 004 | 006 | entional | 9,061 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | 90    |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 186   |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2008 | [200 | *[2 |      |        |
|     |     |          |       | ]{.un | 9]{. | 010 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | *119  |       |      |     |      |        |
| 005 | 007 | entional | ,002* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | *20   |       |      |     |      |        |
|     |     |          | ,687* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 91    |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       | **    | **   | *   |      |        |
|     |     |          |       | [2009 | [201 | *[2 |      |        |
|     |     |          |       | ]{.un | 0]{. | 011 |      |        |
|     |     |          |       | derli | unde | ]{. |      |        |
|     |     |          |       | ne}** | rlin | und |      |        |
|     |     |          |       |       | e}** | erl |      |        |
|     |     |          |       |       |      | ine |      |        |
|     |     |          |       |       |      | }** |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 2   | 2   | Conv     | *260  |       |      |     |      |        |
| 006 | 008 | entional | ,000* |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Captive  | *0*   |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 28    |       |      |     |      |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+

^1^ No survey in Vey Meadows. Unknown number of redds.

^2^ 76,941 were released as parr.

^3^ Does not include 50,100 smolts lost in acclimation pond accident
prior to release

^4^ 32,800 released as parr in Sheep Creek

^5^ 11,800 smolts were lost in downstream trap accident

**Appendix M. Preliminary data from Imnaha River releases between 1982
and 2007.**

+-----+-----+----------+-------+-------+------+-----+------+--------+
| #   | **  |          |       | *     |      |     | *    | *      |
|  Br | Rel |          |       | *Retu |      |     | *Tot | *SAR** |
| ood | eas |          |       | rns** |      |     | al** |        |
|     | e** |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| **  | **  | *        | ### N | **Age | *    | **  | **   | **Per  |
| Yea | Yea | *Release | umber | 3**   | *Age | Age | Retu | cent** |
| r** | r** | Type**   |       |       | 4**  | 5** | rn** |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 2     | 195   | 48   | 4   | 247  | 0.9911 |
| 982 | 984 | entional | 4,920 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 129   | 358   | 704  | 147 | 1    |        |
|     |     | al-Redds |       |       |      |     | ,209 |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 11    | 24    | 18   | 38  | 80   | 0.0691 |
| 983 | 985 | entional | 5,830 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 95    | 77    | 406  | 580 | 1    |        |
|     |     | al-Redds |       |       |      |     | ,063 |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 3     | 55    | 40   | 16  | 111  | 0.3168 |
| 984 | 986 | entional | 5,035 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 119   | 14    | 129  | 154 | 297  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 12    | 101   | 96   | 9   | 206  | 0.1668 |
| 985 | 987 | entional | 3,530 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 462   | 40    | 189  | 81  | 310  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 19    | 183   | 269  | 46  | 498  | 0.2502 |
| 986 | 988 | entional | 9,066 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 284   | 59    | 184  | 97  | 340  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 14    | 69    | 228  | 65  | 362  | 0.2544 |
| 987 | 989 | entional | 2,320 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 183   | 15    | 133  | 91  | 239  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Convent  | 25    | 260   | 551  | 440 | 1    | 0.4944 |
| 988 | 990 | ional^1^ | 3,042 |       |      |     | ,251 |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 237   | 19    | 150  | 349 | 518  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 26    | 100   | 472  | 98  | 670  | 0.2503 |
| 989 | 991 | entional | 7,670 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 116   | 18    | 133  | 71  | 222  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 26    | 24    | 64   | 12  | 100  | 0.0381 |
| 990 | 992 | entional | 2,500 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 115   | 5     | 65   | 7   | 77   |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 15    | 11    | 76   | 12  | 99   | 0.0628 |
| 991 | 993 | entional | 7,659 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 178   | 2     | 95   | 35  | 132  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 43    | 102   | 94   | 7   | 203  | 0.0463 |
| 992 | 994 | entional | 8,699 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 240   | 140   | 289  | 40  | 469  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Convent  | 39    | 91    | 351  | 62  | 504  | 0.1278 |
| 993 | 995 | ional^2^ | 4,255 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 468   | 14    | 106  | 154 | 274  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 9     | 7     | 31   | 4   | 42   | 0.0460 |
| 994 | 996 | entional | 1,240 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 154   | 6     | 104  | 48  | 158  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 5     | 161   | 585  | 32  | 778  | 1.5282 |
| 995 | 997 | entional | 0,911 |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 60    | 31    | 155  | 46  | 232  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     |          |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
| 1   | 1   | Conv     | 9     | 689   | 530  | 131 | 1    | 1.450  |
| 996 | 998 | entional | 3,108 |       |      |     | ,350 |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+
|     |     | Natur    | 136   | 88    | 318  | 314 | 719  |        |
|     |     | al-Redds |       |       |      |     |      |        |
+-----+-----+----------+-------+-------+------+-----+------+--------+

^1^ Includes only Ad marked hatchery releases and returns.

^2^ Does not include 195,814 smolts released with LV mark.

**Appendix N. continued.**

+-----+-----+----------+-------+------+------+-----+------+--------+
| #   | **  |          |       | **R  |      |     | *    | *      |
|  Br | Rel |          |       | etur |      |     | *Tot | *SAR** |
| ood | eas |          |       | ns** |      |     | al** |        |
|     | e** |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| **  | **  | *        | **Num | *    | *    | **  | **   | **Per  |
| Yea | Yea | *Release | ber** | *Age | *Age | Age | Retu | cent** |
| r** | r** | Type**   |       | 3**  | 4**  | 5** | rn** |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 1   | 1   | Conv     | 19    | 1    | 2    | 343 | 3    | 2.007  |
| 997 | 999 | entional | 4,967 | ,091 | ,479 |     | ,913 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 224   | 340  | 2    | 359 | 2    |        |
|     |     | al-Redds |       |      | ,245 |     | ,944 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 1   | 2   | Conv     | 17    | 1    | 3    | 1,  | 5    | 3.028  |
| 998 | 000 | entional | 9,716 | ,153 | ,212 | 077 | ,442 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 143   | 107  | 633  | 709 | 1    |        |
|     |     | al-Redds |       |      |      |     | ,449 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 1   | 2   | Conv     | 12    | 469  | 1    | 67  | 1    | 1.380  |
| 999 | 001 | entional | 3,014 |      | ,162 |     | ,698 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 189   | 111  | 862  | 82  | 1    |        |
|     |     | al-Redds |       |      |      |     | ,055 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     | 30    | 1616 | 1199 | 106 | 2    | 0.9617 |
| 000 | 002 | entional | 3,737 |      |      |     | ,921 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 261   | 113  | 345  | 75  | 533  |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     | 26    | 747  | 957  | 33  | 1    | 0.6471 |
| 001 | 003 | entional | 8,426 |      |      |     | ,737 |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 635   | 27   | 225  | 29  | 281  |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     | 39    | 238  | 1006 |     | 1    | *0     |
| 002 | 004 | entional | 8,185 |      |      |     | ,244 | .3124* |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 1,111 | 14   | 213  |     | 227  |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     | 43    | 156  |      |     | *    |        |
| 003 | 005 | entional | 5,186 |      |      |     | 156* |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 726   | 15   |      |     | *15* |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     | 44    |      |      |     |      |        |
| 004 | 006 | entional | 8,268 |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 495   |      |      |     |      |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     |       |      |      |     |      |        |
| 005 | 007 | entional |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 349   |      |      |     |      |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     |          |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
| 2   | 2   | Conv     |       |      |      |     |      |        |
| 006 | 008 | entional |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+
|     |     | Natur    | 235   |      |      |     |      |        |
|     |     | al-Redds |       |      |      |     |      |        |
+-----+-----+----------+-------+------+------+-----+------+--------+