Survey Plans

Submittal Form

## Approvals

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George Ison Date

Ship Operations Contract Project Officer, Ship

Safety Officer

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Glenn Warren Date

Environmental Monitoring and Indicators Team Leader

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Paul Horvatin Date

Monitoring Indicators and Reporting Branch Chief

# IFYLE-2005 

# August Transect-Diel Cruise (2)

**Dates**: **August 27, 2005 -- August 31, 2005**

**Vessel: Lake Guardian**

#### 

#### Ports:

**Embark:** Cleveland Port Authority: 1100 EDT August 27

**Disembarkment:** Cleveland Port Authority: 1454 EDT August 31

## Cruise Participants:

PIs (indicate project PI if PI will not be on board) Status Cabin
Citizen

  --------------------------------------------------- --------- ------ -----
  \(M\) Henry Vanderploeg (Chief Scientist)           PI               US

  \(M\) Hal Gunder (with S. Brandt)                   Student          US

  \(M\) Jesse Kipp (for S. Ruberg / H. Vanderploeg)   Student          US

  \(F\) Sheena Ripple (with H. Carrick)               Student          US

  \(M\) Hunter Carrick                                PI               US

  \(M\) Dave Fanslow                                  Support          US

                                                                       

                                                                       

                                                                       

                                                                       
  --------------------------------------------------- --------- ------ -----

**[Purpose of Cruise:]{.underline}**

To determine the vertical distribution of fish, mesozooplankton,
phytoplankton, and microbes in central Lake Erie relative to physical
habitat features (e.g., oxygen, temperature).

Objectives:

1.  Monitor 3 diel stations in central Lake Erie for 24 hours each using
    fish acoustics, a plankton survey system (PSS), zooplankton net and
    pump sampling, flow cytometry, and incubation and grazing
    experiments.

2.  Monitor 3 east-west transects using the PSS and acoustics with
    periodic stops to do a CTD. An additional horizontal transect will
    be sampled as well.

Environmental questions asked by the project:

> -How does low dissolved oxygen influence primary
> production/respiration, microbes, mesozooplankton, and fish
> distributions, feeding behavior, condition, and production?

# Survey Plan

## Chemical background information:

[Fanslow/Vanderploeg]{.underline}

Sugar formalin (10%)

Sugar formalin (10%) with Rose Bengal

[Hunter Carrick]{.underline}

Sodium thiosulfate

Manganese chloride

Sodium hydroxide

Sodium iodide

Sulfuric acid

Potassium iodate

Lugols solution (iodine)

Formaldehyde

Glutaraldehyde

Paraformaldehyde

Sodium cacodylate

Primuline

Trizma hydrochloride

Acridine orange

Sugar formalin (formaldehyde)

DCMU (diuron)

Ampicillin (antibiotic)

#### 

#### Core measurements for surveys:

***Along east-west transects and diel site transect***s, while underway:

+---------------+-----------------+-----------------+-----------------+
| Variable      | PI              | Lake Guardian   | Instr           |
|               |                 | Staff Help?     | ument/Technique |
+---------------+-----------------+-----------------+-----------------+
| Continuous    | K               | Yes, with       | To-yo Plankton  |
| temp., DO,    | ipp/Vanderploeg | A-frame and to  | Survey System   |
| Par, ZP       |                 | run PSS         | (PSS)           |
|               | (for            |                 |                 |
|               | Vanderploeg)    |                 |                 |
+---------------+-----------------+-----------------+-----------------+
| Fish Biomass  | Gunder/Fanslow  | Yes, with       | Towed acoustics |
|               |                 | port-side crane | fish (1m below  |
|               |                 |                 | surface)        |
+---------------+-----------------+-----------------+-----------------+
| DO, Temp,     | All             | Yes             | CTD             |
| chla...       |                 |                 |                 |
+---------------+-----------------+-----------------+-----------------+

***At endpoints and midpoints of diel transects (only diel stations not
sampled last week***)

+----------------+----------------+------------------+-----------------+
| Variable       | PI             | Lake Guardian    | Instr           |
|                |                | Staff Help?      | ument/Technique |
+----------------+----------------+------------------+-----------------+
| Depth          | All            | Yes, with        | CTD25, Seabird  |
| profile**s**   |                | starboard side   | fluorometer,    |
| for temp., DO, |                | winch            | Rosette sampler |
| tr             |                |                  |                 |
| ansmissometry, |                |                  |                 |
| fluorometry,   |                |                  |                 |
| etc.           |                |                  |                 |
+----------------+----------------+------------------+-----------------+
| Secchi Depth   | All            | Yes              | Secchi Disk (in |
|                |                |                  | shadow)         |
+----------------+----------------+------------------+-----------------+
| Zooplankton    | Kipp/V         | Yes, with        | 64um net &      |
| net (whole     | anderploeg(for | starboard side   | 153um net       |
| water column)  | Vanderploeg)   | winch at 1:00 &  | vertical haul   |
|                |                | 13:00 only       |                 |
|                |                |                  | (n=2 reps of    |
|                |                |                  | each)           |
+----------------+----------------+------------------+-----------------+
| Discrete       | Kipp/V         | Yes, with        | Diaphragm pump  |
| wat            | anderploeg(for | starboard side   | (need           |
| er/zooplankton | Vanderploeg)   | winch            | compressed air) |
+----------------+----------------+------------------+-----------------+
| Benthic        | Gunder/Fanslow | Yes, with        | Ponar sampler   |
| macroinverts.  | (for           | starboard side   | with 250-um     |
|                | Ludsin/Hook)   | winch            | elutriator      |
|                |                |                  | ([n=3]{.mark}   |
|                |                |                  | reps per        |
|                |                |                  | stopover site)  |
+----------------+----------------+------------------+-----------------+
| Phytoplankton  | Carrick        | Yes, with        | Rossette        |
| cell           |                | starboard side   | sampler (20     |
| nu             |                | winch            | liters of water |
| mbers/biomass, |                |                  | required) &     |
| algal          |                |                  | on-board        |
| pigments,      |                |                  | incubation      |
| sestonic       |                |                  |                 |
| nutrients, and |                |                  |                 |
| primary        |                |                  |                 |
| production and |                |                  |                 |
| respiration    |                |                  |                 |
| rates          |                |                  |                 |
+----------------+----------------+------------------+-----------------+
| Quantify in    | Carrick        | Yes, deploying   | Diurnal         |
| situ           |                | lighted mooring  | Light-Dark      |
| photosynthesis |                | from zodiac and  | Bottle          |
| and            |                | with starboard   | Experiments     |
| respiration    |                | side winch       | attached to     |
| (stations 7    |                |                  | moored light,   |
| (Diel_B), 8, & |                |                  | Rosette         |
| 13 (Diel_D)    |                |                  | Sampler;        |
|                |                |                  | incubators      |
+----------------+----------------+------------------+-----------------+
| Mi             | Carrick        | Yes, with        | Rosette         |
| crozooplankton |                | starboard side   | Sampler,        |
| Grazing        |                | winch            | incubators      |
| (stations      |                |                  |                 |
| 7-Diel_B &     |                |                  |                 |
| 13-Diel_D)     |                |                  |                 |
+----------------+----------------+------------------+-----------------+

## Equipment to be brought and used on deck (investigators):

##  (Equipment marked with an \* will be provided by the individual PI)

-   EBT, SeaBird fluorometer, Rosette sampler with Go Flow bottles EBT,
    DO, Conductivity, pH, Transmissometer (multiple)

-   Air Compressor (multiple)

-   Secchi disk (multiple)

-   \*Diaphragm pump: for pumping water at discrete depths
    (Vanderploeg/Kipp)

-   \*Plankton survey system (Kipp)

-   \*Acoustics system (Gunder/Fanslow)

-   \*64 and 153 µm zooplankton nets (Vanderploeg/Kipp)

```{=html}
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-   \*Research grade compound microscope (Leica DMR) (Carrick)

-   \*Automated Titrator (Brinkman Titrino) (Carrick)

-   \*Fluorometer (10-Au) (Carrick)

-   \*Laptop computers (Carrick)

-   \*Filtering manifolds with pumps (Carrick)

## Mooring Requirements:

Lighted moorings will be placed at diel stations B (near mooring 7) and
D (near mooring 13) (and possibly at a 3^rd^ diel station, but only if
arrived at before sunrise) in order to do *in situ* photosynthesis and
respiration experiments using light and dark bottles. **A mooring with a
chain of bottles will need to be deployed just prior to sunrise and
retrieved just prior to sunset** **at both stations. These experiments
require that the Lake Guardian is on each diel station prior to sunset.
A zodiac will be needed to deploy the moorings.**

## Diving Operations:

No diving operations are currently planned for this cruise.

## Special Operating Requirements:

For our east-west transects that span most of the lake (Transects from A
to C to D and A to H), we would like to tow instruments (PSS, acoustics)
at **5.25 knots** (if acoustic gear is not losing pings). Occasional
stops to do a CTD will need to be made along the way (at A, midpoint of
A-C, and at station C).

For the diel sampling our experimental sites are actually a 3 nautical
mile transects with sampling occurring continuously along each transect,
and at both ends (\~1000 m outside the transect line). We will sample
each 3 nautical mile transect for 24 hr, with sampling blocks divided
into the following intervals (EDT):

**0100-0500; 0500-0900; 0900-1300; 1300-1700; 1700-2100; 2100-0100**.

We would like to complete six 24-hour experiments at 3 sites in the
central basin (Diel stations B, D, and A). Ideally, ship speed will be
**4 knots**, when on a transect.

**Sampling Protocol for Transect Sampling:**

Along each east-west transect run, we would like to do the following:

1.  At start of transect (during daytime)

    a.  Do a CTD cast to measure temperature, Par, oxygen, conductivity,
        etc..

2.  With ship not moving (or only drifting), deploy PSS (from A-frame on
    stern to depth of 4 m) and acoustics (side from crane to depth of
    1 m) at start of transect line (note, an air compressor hose will be
    attached to the acoustics towfish to collect water continuously).

3.  Tow PSS and acoustics along transect (ideally at 5.25 knots) until
    mid-point or end of transect (as outlined in Table 1 below).

    a.  Please notify PSS and acoustics operators when 10, 5, 1 and then
        0 minutes from midpoint (or end) of transect

    b.  Continue steaming at 4 knots through the end of the transect
        until PSS is parked at a depth of 4 m

    c.  Once up at 4 m, we will notify captain, who then can turn around
        and/or "park" ship

**PSS and acoustics equipment can remain in the water during the entire
24 hr sampling period, but should be pulled up during the steam to the
next station.**

**Sampling Protocol for Diel Experiment:**

At each station, we would like to do the following:

1.  With ship not moving (or only drifting), deploy PSS (from A-frame on
    stern to depth of 4 m) and acoustics (side from crane to depth of
    1 m) at \~1000 m before first station (i.e., outside transect line).

2.  Tow PSS and acoustics along a 3 nautical mile transect (ideally at
    \~4 knots, 5 knots max). Transect should take about 45-50 min. at 4
    knots. End at coordinates on the opposite end of transect.

    -   Please notify PSS and acoustics operators when 10, 5, 1 and then
        0 minutes from end of transect

    -   Continue steaming at 4 knots through the end of the transect
        until PSS is parked at a depth of 4 m

    -   Once up at 4 m, we will notify captain, who then can turn around
        and/or "park" ship \~1000 m from the end of the transect (i.e.,
        park ship outside of transect line)

> **PSS and acoustics equipment can remain in the water during the
> entire 24 hr sampling period, but should be pulled up during the steam
> to the next station.**

3.  Once at a stop and waters are settled, conduct a CTD cast to
    determine vertical structure.

4.  Use a rosette sampler to collect water at different depths (2-4
    depths) for all PIs

    -   Water for grazing experiments, chlorophyll

5.  Duplicate zooplankton net tows, full water column, only two times
    during the diel period; at1:00 with 64um net, and at 13:00 with both
    64 & 153 um nets.

6.  Pump for zooplankton at no more than 4 depths (to be determined from
    CTD cast).

7.  Collect benthic macroinvertebrates with ponars (n=3 reps/diel site

    -   Benthos should be collected at both endpoints and the midpoint
        of each diel transect (n=9 samples/diel transect)

    -   Benthic collections only need to be made during 1 cycle of the
        24-hr period (n=9 total samples)

8.  Just prior to sunrise a lighted mooring will need to be deployed for
    light-dark bottle experiments

9.  Sit and wait until next cycle to start, per times above.

10. Repeat the cycle until 6 cycles have been conducted at the site (24
    hr).

11. During each cycle in the daytime, we need an extra 30 minutes with
    acoustics over the side (at surface, but not towed) to calibrate the
    instrument. This can only occur in calm conditions.

12. Move to next station and repeat.

Attached at the end, are Hunter Carrick's explicit requests:

**In theory, time will permit all stations to be sampled by the early
afternoon of August 31. Below is the prioritizations of stations to be
sampled (i.e., 1^st^ stations are more important than last stations).**

1.  Transect B

2.  Transect D

3.  Transect H or A (or another with a thick, hypoxic hypolimnion)

4.  East-west transect from A to C to D

5.  Transect from A to H.

See Table 2 for sampling station coordinates and Table 3 and Figure 1
for the project cruise track.

# Lab Space Requests (if known):

Vanderploeg/Kipp: Back Lab for PSS computer and joy stick (port side)

> freezer space

Filtering space for chlorophyll samples

Lab space for water filtration

Deck space for diaphragm pump (starboard side)

Gunder/Fanslow Back Lab area for acoustics computer (port side)

Deck space for towfish

Carrick: Bench space in primary productivity (biology) lab for
microscope

> Deck space for decktop incubator

# 

# 

# Hunter Carrick's email correspondence (verbatim):

# Hi Stu:  Good talking with you this afternoon.  My chem and equipment list is enclosed. My primary research interests are listed below including a brief overview of required sampling and facilities.  I would request to work in the primary production lab and have some space where i might do microscope work.  I will need one bunk (for myself) and deck space (see below) for one decktop incubator.  1.  Ambient water column conditions (Stations 5, 7, 8, and 13) I will need to take CTD casts at each station to assess ambient conditions Whole water samples (20-liters) will be collected from epi, meta, and hypolimnia. I will subsample collected water to estimates: phytoplankton cell numbers/biomass, algal pigments, sestonic nutrients, and primary production and respiration rates. The production/resp experiments will conducted by incubating bottles (light and dark 300ml BOD bottles) in a deck top incubator (I will provide).  The incubator was placed on the aft deck behind the pilot house (power and deck water needed).  2.  Diurnal Light-Dark Bottle Experiments (stations 7 and 13) I will perform bottle experiments to estimate the rates (simultaneous) of primary prod and resp. in the water column. Whole water samples (15-liters) will be collected from epi, meta, and hypolimnia just prior to dusk. Water from each depth will be placed into twelve 300ml BOD bottles (36 bottles total).  One set will be place on a moored light that will be deployed back in the lake at sunrise.  The other set (18 bottles) will be incubated in the Percival incubator (PPr Lab).  This will require that one of the two Percival incubators will run at hypolimnetic light and temperature conditions (ca 10 oC and \<5 ue/m2/s). The moored light will be retrieved at sunset that day.  3. Zooplankton grazing experiments (stations 7 and 13) I will perform bottle experiments (8 1-liter bottles) to estimate zoop grazing on phytoplankton that need to be incubated at surface irradiance in the Percival incubators (PPr Lab). This will require collecting appr. 10-liters of water from the epilimnion and two zooplankton tows (10m to surface with 153um Wis net).  4.  Microzooplankton grazing experiments (stations 7 and 13) I will perform bottle experiments (12 1-liter bottles) to estimate microzoop grazing on picoplankton that need to be incubated at surface irradiance in the Percival incubators (PPr Lab). This will require collecting appr. 10-liters of water from the epilimnion  Should you have any questions, feel free to contact me (cell 814-883-1238 or numbers below). Thanks, Hunter

# 

# 

# Master Sample Sites and Location:

**Master list of stations.**

  ----------------- ------------- ----------- ----------- -------------------- ----------
                                  **Decimal               **Degree-Minutes**   
                                  Degrees**                                    

  **Description**   **Acronym**   **Lat**     **Lon**     **Lat**              **Lon**

                    Cleveland     41.500      81.700      41.300               81.420

  Mooring 8         Buoy8         41.833      82.200      41.500               82.120

  Diel_A            Diel_AE       41.990      82.058      41.594               82.035

                    Diel_AM       41.990      82.092      41.594               82.055

                    Diel_AW       41.990      82.126      41.594               82.076

  Diel_B            Diel_BE       41.966      81.650      41.580               81.390

                    Diel_BM       41.966      81.688      41.580               81.413

                    Diel_BW       41.966      81.718      41.580               81.431

  Diel_D            Diel_DE       42.200      80.810      42.120               80.486

                    Diel_DM       42.200      80.844      42.120               80.506

                    Diel_DW       42.200      80.878      42.120               80.527

  Diel H            Diel_HW       41.8520     -82.3950    41.51                82.23.7

                    Diel_HM       41.8520     -82.3600    41.51                82.21.6

                    Diel_HE       41.8520     -82.3250    41.51                82.19.5

  Diel C            Diel_CW       42.1750     -81.2667    42.10.5              81.16

                                                                               

                                                                               

                                                                               

                                                                               

                                                                               
  ----------------- ------------- ----------- ----------- -------------------- ----------

# Projected Cruise Plan

Sampling coordinates and estimated sampling times for Aug 27-31, 2005
Lake Guardian diel cruise. **I assumed that sampling occurred at 4 knots
while on a diel stations, at 5.25 knots when doing long PSS/acoustics
transects between stations, and at 8 knots during transit between
stations (no sampling).**

  ---- ----------- ---------- ----------- -------- ------- --------- ------------------- --------- ---------- -------
  \#   Station     Latitude   Longitude   Date     ETA     Station   Tasks               Transit   Transit    speed
       name                                                Time (hr)                     time      distance   knots
                                                                                         (hr)\*               

  0    Cleveland   41.500     -81.700     27-Aug   11:00   0         Depart Cleveland              0           

  1    AE          41.9900    -82.0583    27-Aug   14:50   0.5       CTD/PSS/acoustics   3.34      33.4       10

  2    A-C mid     42.0797    -81.6990    27-Aug   18:32   0.5       CTD/PSS/acoustics   3.20      16.8       5.25

  3    CW          42.1750    -81.3342    27-Aug   22:17   0.5       CTD/PSS/acoustics   3.26      17.1       5.25

  4    DW          42.2000    -80.8775    28-Aug   2:21    0         PSS/acoustics       4.05      20.3       5

  5    DE          42.2000    -80.8100    28-Aug   5:01    1.93      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  6    DE          42.2000    -80.8100    28-Aug   5:01    0         PSS/acoustics       0.00      0.0        4

  7    DW          42.2000    -80.8775    28-Aug   9:01    3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  8    DW          42.2000    -80.8775    28-Aug   9:01    0         PSS/acoustics       0.00      0.0        4

  9    DE          42.2000    -80.8100    28-Aug   13:01   3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  10   DE          42.2000    -80.8100    28-Aug   13:01   0         PSS/acoustics       0.00      0.0        4

  11   DW          42.2000    -80.8775    28-Aug   17:00   3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  12   DW          42.2000    -80.8775    28-Aug   17:00   0                             0.00      0.0        4

  13   DE          42.2000    -80.8100    28-Aug   21:00   3.25      CTD/rosette 1m pump 0.75      3.0        4
                                                                     (Tracy)                                  

  14   DE          42.2000    -80.8100    28-Aug   21:00   0         PSS/acoustics       0.00      0.0        8

  15   DW          42.2000    -80.8775    29-Aug   0:15    2.5       CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  16   BE          41.9658    -81.6500    29-Aug   5:01    1.07      PSS/acoustics       3.70      37.0       10

  17   BW          41.9658    -81.7175    29-Aug   9:01    3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  18   BW          41.9658    -81.7175    29-Aug   9:01    0         PSS/acoustics       0.00      0.0        4

  19   BE          41.9658    -81.6500    29-Aug   13:01   3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  20   BE          41.9658    -81.6500    29-Aug   13:01   0         PSS/acoustics       0.00      0.0        4

  21   BW          41.9658    -81.7175    29-Aug   17:00   3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  22   BW          41.9658    -81.7175    29-Aug   17:00   0         PSS/acoustics       0.00      0.0        4

  23   BE          41.9658    -81.6500    29-Aug   21:00   3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  24   BE          41.9658    -81.6500    29-Aug   21:00   0         PSS/acoustics       0.00      0.0        4

  25   BW          41.9658    -81.7175    30-Aug   1:00    3.25      CTD/rosette 1m pump 0.75      3.0        4
                                                                     (Tracy)                                  

  26   BW          41.9658    -81.7175    30-Aug   1:00    0         PSS/acoustics       0.00      0.0        4

  27   BE          41.9658    -81.6500    30-Aug   5:00    3.25      CTD/rosette ZP      0.75      3.0        4
                                                                     pump/net Secchi                          

  28   AE          41.9900    -82.0583    30-Aug   9:32    0         PSS/acoustics       4.53      18.1       4

  29   AW          41.9900    -82.1257    30-Aug   13:01   2.75      CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  30   AW          41.9900    -82.1257    30-Aug   13:01   0         PSS/acoustics       0.00      0.0        10

  31   AE          41.9900    -82.0583    30-Aug   17:01   3.25      CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  32   AE          41.9900    -82.0583    30-Aug   17:01   0         PSS/acoustics       0.00      0.0        4

  33   AW          41.9900    -82.1257    30-Aug   21:01   3.25      CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  34   AW          41.9900    -82.1257    30-Aug   21:01   0         PSS/acoustics       0.00      0.0        4

  35   AE          41.9900    -82.0583    31-Aug   1:00    3.25      CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  36   AE          41.9900    -82.0583    31-Aug   1:00    0         PSS/acoustics       0.00      0.0        4

  37   AW          41.9900    -82.1257    31-Aug   5:00    3.25      CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  38   AE          41.9900    -82.0583    31-Aug   5:22    0         PSS/acoustics       0.37      3.0        8

  39   AW          41.9900    -82.1257    31-Aug   8:37    2.5       CTD/rosette ZP      0.74      3.0        4
                                                                     pump/net Secchi                          

  40   HE          41.852     -82.3250    31-Aug   11:25   0.5       CTD/PSS/acoustics   2.30      12.1       5.25

  41   Cleveland   41.500     -81.700     31-Aug   14:54   0         End cruise          3.48      34.8       10
  ---- ----------- ---------- ----------- -------- ------- --------- ------------------- --------- ---------- -------

# Numbers denote the projected cruise track that corresponds to the table below it. 

![](media/image1.wmf){width="9.34291338582677in"
height="3.707086614173228in"}