**REPORTS OF THE COORDINATORS**

**Overall coordinator's report**

**Udda Lundqvist**

**Nordic Genetic Resource Center**

**P.O. Box 41, SE-230 53 Alnarp**

**e-mail: <udda@nordgen.org>**

Since the latest overall coordinator's report in Barley Genetics
Newsletter Volume 37 many of us met at the 10th International Barley
Genetics Symposium in Alexandria, Egypt, during during 6 days in the
beginning of April 2008. About 300 participants attended the meetings,
16 different sessions and 5 workshops were arranged and a number of 100
posters were presented. We could get much information of many
interesting papers with new and interesting results for the barley
community.

As Overall Coordinator I arranged a workshop on 'Barley Genetic Linkage
Groups, Barley Genome, Genes and Genetic Stocks'. Discussions were
focused on the coordination system of to-day and the future and it was
stressed if the whole genome should be coordinated by one person. After
intensive discussions it was decided that for the time being it was not
ready to do this for one person. Therefore it was recommended to
continue with to-days system. Some changes of the coordinators have
taken place. Victoria Carollo Blake, Bozeman, Montana State University,
USA, offered herself to take care of chromosome 6H instead of Duane Falk
who is not engaged in barley work any more. Andy Kleinhofs, USA, who has
taken care of coordinating the integration of molecular and
morphological barley maps wanted to step down and he got replaced by
David Marshall from the Genetics Programme at the Scottish Crop Research
Institute, Invergowrie, Dundee, United Kingdom. Also Brian Steffenson,
USA, the coordinator for disease and pest resistant genes wanted to step
down and got replaced by Mark Sutherland, Australia. I want to take the
opportunity and thank the retired coordinators for their willingness to
provide us with all important barley information and their coorporation.
Regarding the Barley Genetics Newsletter the workshop decided after some
discussions to continue in electronic format as it is the only forum for
the barley community to publish gene descriptions and short research
notes. A summarizing report of the workshop is published in this issue
of Barley Genetics Newsletter.

As recommended at the 10th International Barley Genetics Symposium the
rules for Nomenclature and Gene Symbolization in Barley is published in
this volume of BGN. Tables of Barley Genetic Stock descriptions by BGS
numbers (Table 1) and by locus symbols in alphabetic order (Table 2) are
again published in this volume. They are necessary for barley
researchers to find important information in the AceDB database and
GrainGenes.

**List of Barley Coordinators**

**Chromoosome 1H (5):** Gunter Backes, The University of Copenhagen,
Faculty of Life Science, Department of Agricultural Sciences,
Thorvaldsensvej 40, DK-1871 Fredriksberg C, Denmark. FAX: +45 3528 3468;
e-mail: \<[guba@life.ku.dk\>]{.underline}

**Chromosome 2H (2):** Jerry. D. Franckowiak, Hermitage Research
Station, Queensland Department of Primary Industries and Fisheries,
Warwick, Queensland 4370, Australia, FAX: +61 7 4660 3600; e-mail:
\<[Jerome.Franckowiak@dpi.qld.gpv.au]{.underline}\>

**Chromosome 3H (3):** Luke Ramsey, Genetics Programme, Scottish Crop
Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX:
+44 1382 562426. E-mail: \<[Luke.Ramsey@scri.sari.ac.uk]{.underline}\>

**Chromosome 4H (4):** Arnis Druka, Genetics Programme, Scottish Crop
Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX:
+44 1382 562426. e-mail: \<[adruka@scri.sari.ac.uk]{.underline}\>

**Chromosome 5H (7):** George Fedak, Eastern Cereal and Oilseed Research
Centre, Agriculture and Agri-Food Canada, ECORC, Ottawa, ON, Canada K1A
0C6, FAX: +1 613 759 6559; e-mail: \<[fedakga@agr.gc.ca]{.underline}\>

**Chromosome 6H (6):** Victoria Carollo Blake, Plant Sciences and Plant
Pathology, Montana State University, Bozeman, MT 59717, USA.e-mail:
\<[vblake@montana.edu]{.underline}\>

**Chromosome 7H (1):** Lynn Dahleen, USDA-ARS, State University Station,
P.O. Box 5677, Fargo, ND 58105, USA. FAX: + 1 701 239 1369; e-mail:
\<[DAHLEENL@fargo.ars.usda.gov]{.underline}\>

**Integration of molecular and morphological marker maps:** David
Marshall, Genetics Programme, Scottish Crop Research Institute,
Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX: 44 1382 562426.
e-mail: \<[David.Marshall@scri.ac.uk]{.underline}\>

**Barley Genetics Stock Center:** Harold Bockelman, USDA-ARS, National
Small Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID
83210, USA. FAX: +1 208 397 4165; e-mail:
\<[nsgchb@ars-grin.gov]{.underline}\>

**Trisomic and aneuploid stocks:** Harold Bockelman, USDA-ARS, National
Small Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID
83210, USA. FAX: +1 208 397 4165; e-mail:
\<[nsgchb@ars-grin.gov]{.underline} \>

**Translocations and balanced tertiary trisomics:** Andreas Houben,
Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3,
DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail:
\<[houben@ipk-gatersleben.de]{.underline}\>

**List of Barley Coordinators (continued)**

**Desynaptic genes:** Andreas Houben, Institute of Plant Genetics and
Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany.
FAX: +49 39482 5137; e-mail: \<[houben@ipk-gatersleben.de]{.underline}\>

**Autotetraploids:** Wolfgang Friedt, Institute of Crop Science and
Plant Breeding, Justus-Liebig-University, Heinrich-Buff-Ring 26-32,
DE-35392 Giessen, Germany. FAX: +49 641 9937429; e-mail:
\<[wolfgang.friedt@agrar.uni-giessen.de]{.underline}\>

**Disease and pest resistance genes:** Mark Sutherland, Centre for
Systems Biology, University of Southern Queensland, Toowoomba Q 4350,
Australia. FAX: +61 7 4631 1530. e-mail:
\<[marksuth@usg.edu.au]{.underline}\>

**Eceriferum genes:** Udda Lundqvist, Nordic Genetic Resource Center,
P.O. Box 41, SE-230 53 Alnarp, Sweden. FAX:.+46 40 536650; e-mail: \<
[udda@nordgen.org]{.underline}\>

**Chloroplast genes:** Mats Hansson, Carlsberg Research Center, Gamle
Carlsberg vej 10, DK-2500 Valby, Copenhagen Denmark. e-mail:
\<[mats@crc.dk]{.underline}\>

**Genetic male sterile genes:** Mario C. Therrien, Agriculture and
Agri-Food Canada, P.O. Box 1000A, R.R. #3, Brandon, MB, Canada R7A 5Y3,
FAX: +1 204 728 3858; e-mail: \<[MTherrien@agr.gc.ca]{.underline}\>

**Ear morphology genes:** Udda Lundqvist, Nordic Genetic Resource
Center, P.O. Box 41, SE-230 53 Alnarp, Sweden. FAX: +46 40 536650;
e-mail: \< [udda@nordgen.org](mailto: udda@nordgen.org)[\>]{.underline}

or

Antonio Michele Stanca: Istituto Sperimentale per la Cerealicoltura,
Sezione di Fiorenzuola d'Arda, Via Protaso 302, IT-29017 Fiorenzuola
d'Arda (PC), Italy. FAX +39 0523 983750, e-mail:
\<[michele@stanca.it]{.underline}\>40 536650

**Semi-dwarf genes:** Jerry D. Franckowiak, Hermitage Research Station,
Queensland Department of Primary Industries and Fisheries, Warwick,
Queensland 4370, Australia, FAX: +61 7 4660 3600; e-mail: \<
[Jerome.Franckowiak@dpi.qld.gpv.au]{.underline} \>

**Early maturity genes:** Udda Lundqvist, Nordic Genetic Resource
Center, P.O. Box 41,

SE-230 53 Alnarp, Sweden. FAX: +46 40 536650; e-mail:
\<[udda@nordgen.org]{.underline}\>

**Barley-wheat genetic stocks:** A.K.M.R. Islam, Department of Plant
Science, Waite Agricultural Research Institute, The University of
Adelaide, Glen Osmond, S.A. 5064, Australia. FAX: +61 8 8303 7109;
e-mail: \<[rislam@waite.adelaide.edu.au]{.underline}\>

**Coordinator's Report: Barley Chromosome 1H (5)**

**Gunter Backes**

**The University of Copenhagen\
Faculty of Life Sciences**

**Department of Agricultural Sciences**

**Thorvaldsensvej 40\
DK-1871 Frederiksberg C, Denmark**

**e-mail: [guba@life.ku.dk]{.underline}**

Hearnden *et al*. (2007) developed a high-density genetic map using DArT
markers and microsatellites in a population of 90 doubled haploid lines
from a cross between the Australian feed barley variety 'Barque-73' and
the *H. vulgare* ssp. *spontaneum* accession 'CPI 71284-48'. The map for
1H includes 90 DArT marker loci, 54 genomic and 25 EST-based SSR marker
loci as well as 2 InDel marker loci (171 marker loci in total).

The high-density barley linkage map of Varshney *et al*. (2007) includes
328 marker loci for chromosome 1H (225 AFLP marker loci, 93 RFLP marker
loci, 41 SSR marker loci, 7 gene loci, one CAP marker locus and one RAPD
marker locus). In contrast to the map presented above, it is a consensus
map based on 6 different mapping populations. It also shows the BIN
structure as defined by Kleinhofs and Graner (2001), but subdivides 10
cM BINs in 2 sub-BINs of 5 cM each. The BINs given in this report relate
to this map, if not mentioned otherwise. Further this map and the
respective segregating populations were used to compare the distribution
of QTLs for resistance against barley leaf rust caused by *Puccinia
hordei* and to compare the detected QTL with defense gene homologs
(Marcel *et al*., 2007). On chromosome 1H they found, using the results
of green-house experiments in the Steptoe/Morex population, the QTL
*Rphq14* in the BINs 1.2 to 2.1 explaining 13% of the phenotypic
variance.

Using RNA from wheat-barley ('Chinese spring'/'Betzes') ditelosomic
addition lines on the Affimetrix Barley 1 GeneChip, Bilgic *et al*.
(2007) localized 1257 barley genes to different chromosome arms. Of
those, 24 transcripts were assigned to chromosome 1HS (23 single-copy
and one multi-copy transcript).

A low phytic acid mutation (*lpa3-1*) causing a reduction of 75% of the
phytic acid content in the sodium-acide induced mutant M635 compared to
the wild-type 'Harrington' was localized to chromosome 1H BIN 12.2
(Roslinsky *et al*., 2007). The localization was carried out using first
20 F~5~ RIL lines of a cross CDC Freedom/M635 in a bulk segregant
analysis, followed by the integration of the resulting partial map into
the Harrington/Morex population from the North American Barley Genome
Mapping Project (Hayes *et al*., 1997).

Mutants related to root hair formation were used to localize the
effected genes in F~2~-progenies from crosses of four different mutant
lines with the varieties 'Steptoe' and 'Morex' (Janiak and Szarejko,
2007). On chromosome 1H, BIN 11.2 or 12.1, *rph1* was localized, causing
the development of root hairs to stop after primordia have been formed.

*FLOWERING LOCUS T*-like (FT) genes play a central role in integrating
flowering signals in Arabidopsis . Based on 13 rice FT gene sequences,
barley homologs were searched in EST databases and five barley FT
sequences were detected and localized (Faure *et al*., 2007). One of
them, *HvFT3* was localized on chromosome 1H, BIN 11 or 12 in a
population of 95 doubled haploid lines from the cross Igri/Triumph
(Laurie *et al*., 1995).

Lee and Neate (2007a) localized resistance genes against Septoria
speckled leaf blotch caused by Septoria passerinii present in the barley
accessions 'Clho 1300' (*Rsp1*), 'Clho 4789' (*Rsp2*) and 'Clho 10644'
(*Rsp3*). They crossed each of these accessions to the varieties
'Foster' and 'Robust' and used the resulting F~2~-populations (103 to
125 lines) to greenhouse as well as field experiments and a subsequent
linkage analysis. On 1HS two of the resistances genes were localized:
*Rsp2* to BIN 2.1 and *Rsp3* to BIN 2.2. Because of the low number of
common markers, the latter BIN-localization of Rsp3 is a rough estimate.
The authors also published STS markers linked to those resistance genes
(Lee and Neate, 2007b)

Quantitatively acting resistance genes against the net form of net
blotch, caused by *Pyrenophora teres* were localized by Lehmensieck *et
al*. (2007). They used three different doubled haploid populations (111
to 153 lines) on field trials (2-3 years environments). In the
Arapiled/Franklin population they detected a QTL on chromosome 1HS, BIN
2-3. It had a LOD of 2.9 to 3.3 and explained 9 to 12% of the phenotypic
variation. Arapiles contributed the allele conferring resistance.

Against the same disease, Manninen *et al*. (2006) detected one or
several minor QTLs on 1H in a doubled haploid population (119 lines)
from a cross between the Finnish variety 'Rolfi' and the Ethiopian
accession 'CI 9819'. The region of the chromosome that associated with
net blotch resistance covered BIN 4 to 11.

Panozzo *et al*. (2007) localized QTLs for malting quality related
traits in two different doubled haploid population, originating from the
crosses 'Arapiles' x 'Franklin' and 'Alexis' x 'Sloop' and comprising
225 and 100 lines, respectively. In the Arapiles/Franklin population, 2
QTL were detected on chromosome 1H, one in BIN 6.2 effecting hot water
extract, diastatic power, α-amylase activity, wort β-glucan, wort
viscosity and free α-amino acids and one in BIN 7.1 effecting
β‑glucanase activity and free α-amino acids. In the Alexis/Sloop
population, caused by a lack of common markers with the populations used
for binning, only rough estimates of the BINs can be given. One QTL was
detected in BIN 7 for hot water extract, α -amylase activity, wort
β-glucan, wort viscosity and free α-amino acids. A second QTL in BIN 13
affected α-amylase activity, β‑glucanase activity and free α-amino
acids.

In a population derived from a cross between a Spanish and a US variety
('Beka' x 'Logan') and after field experiment carried out both in Spain
and Scotland, Molina-Cano *et al*. (2007) identified QTLs for β-glucan
content. One of the QTLs, explaining 8 to 15% of the phenotypic
variance, was detected on chromosome 1H, BIN 14.

Seed dormancy QTLs were localized by Hori *et al*. (2007) by means of
measuring the seed germination five and ten weeks after harvest in 7
different RI populations (93-94 lines). QTLs on 1H were found in three
of these seven populations. In Harbin 2-row/Khanaqin 7, one QTL was
detected in BIN 9, in Harbin 2 row/Turkey 45 one QTL was detected in BIN
6 and in Haruna Nijo/H602 one QTL was detected in BIN 5. The QTLs
explained 4%, 12% and 4% of the phenotypic variance, respectively.

An advanced backcross population (BC~2~F~6~) derived from a cross
between 'Harrington' and the wild barley (*H. v.* ssp. *spontaneum*)
accession OUH602 was used by Gyenis *et al*. (2007) to search for QTLs
associated with morphological and agronomic traits measured in a field
experiment on five environments. On chromosome 1H, 3 QTLs were found,
one associated with the fragility of ear rachis in BIN 12, one QTL
associated with plant height in BIN 12 and one QTL for kernel color in
BIN 14 and 15. The QTLs explained 9-54%, 11% and 30% of the phenotypic
variance, respectively. In this paper, the BINs were directly given by
the authors.

**References:**

**Bilgic, H., S. Cho, D.F. Garvin, and G.J. Muehlbauer, 2007.** Mapping
barley genes to chromosome arms by transcript profiling of wheat-barley
ditelosomic chromosome addition lines. Genome 50: 898-906.

**Faure, S., J. Higgins, A. Turner and D.A. Laurie, 2007.** The
FLOWERING LOCUS T-like gene family in barley (*Hordeum vulgare*).
Genetics 176: 599-609.

**Gyenis, L., S.J. Yun, K.P. Smith, B.J. Steffenson, E. Bossolini, M.C.
Sanguineti, and G.J. Muehlbauer, 2007.** Genetic architecture of
quantitative trait loci associated with morphological and agronomic
trait differences in a wild by cultivated barley cross. Genome 50:
714-723.

**Hayes, P.M., J. Cereno, H. Witsenjboer, M. Kuiper, M. Zabeau, K. Sato,
A. Kleinhofs, D. Kudrna, M. Saghai Maroof, D. Hoffman, and N.A.B.G.
Project (1997).** Journal of Agricultural Genomics, Vol. 3.
http://wheat.pw.usda.gov/jag/papers97/paper297/indexp297.html

**Hearnden, P.R., P.J. Eckermann, G.L. McMichael, M.J. Hayden, J.K.
Eglinton, and K.J. Chalmers, 2007.** A genetic map of 1,000 SSR and DArT
markers in a wide barley cross. Theor. Appl. Genet. 115: 383-391.

**Hori, K., K. Sato, and K. Takeda, 2007.** Detection of seed dormancy
QTL in multiple mapping populations derived from crosses involving novel
barley germplasm. Theor. Appl. Genet. 115: 869-876.

**Janiak, A. and I. Szarejko, 2007.** Molecular mapping of genes
involved in root hair formation in barley. Euphytica 157: 95-111.

**Kleinhofs, A. and A. Graner, 2001.** An integrated map of the barley
genome. In: R.L. Phillips & I.K. Vasil (Eds.), DNA marker in plants, pp.
187-199. Kluwer, Dordrecht.

**Laurie, D.A., N. Pratchett, J.H. Bezant, and J.W. Snape, 1995.** RFLP
mapping of 5 major genes and 8 quantitative trait loci controlling
flowering time in a winter x spring barley (*Hordeum vulgare* L.) cross.
Genome 38: 575-585.

**Lee, S.H. and S.M. Neate, 2007a.** Molecular mapping of *Rsp1*,
*Rsp2*, and *Rsp3* genes conferring resistance to septoria speckled leaf
blotch in barley. Phytopathology 97: 155-161.

**Lee, S.H. and S.M. Neate, 2007b.** Sequence tagged site markers to
*Rsp1*, *Rsp2*, and *Rsp3* genes for resistance to septoria speckled
leaf blotch in barley. Phytopathology 97: 162-169.

**Lehmensiek, A., G.J. Platz, E. Mace, D. Poulsen, and M.W. Sutherland,
2007.** Mapping of adult plant resistance to net form of net blotch in
three Australian barley populations. Aust. J. Agr. Res. 58: 1191-1197.

**Manninen, O.M., M. Jalli, R. Kalendar, A. Schulman, O. Afanasenko, and
J. Robinson, 2006.** Mapping of major spot-type and net-type netblotch
resistance genes in the Ethiopian barley line Cl 9819. Genome 49:
1564-1571.

**Marcel, T.C., R.K. Varshney, M. Barbieri, H. Jafary, M.J.D. de Kock,
A. Graner, and R.E. Niks, 2007.** A high-density consensus map of barley
to compare the distribution of QTLs for partial resistance to Puccinia
hordei and of defence gene homologues. Theor. Appl. Genet. 114: 487-500.

**Molina-Cano, J.L., M. Moralejo, M. Elia, P. Munoz, J.R. Russell, A.M.
Perez-Vendrell, F. Ciudad, and J.S. Swanston, 2007.** QTL analysis of a
cross between European and North American malting barleys reveals a
putative candidate gene for beta-glucan content on chromosome 1H. Mol.
Breed. 19: 275-284.

**Panozzo, J.F., P.J. Eckermann, D.E. Mather, D.B. Moody, C.K. Black,
H.M. Collins, A.R. Barr, P. Lim, and B.R. Cullis, 2007.** QTL analysis
of malting quality traits in two barley populations. Aust. J. Agr. Res.
58: 858-866.

**Roslinsky, V., P.E. Eckstein, V. Raboy, B.G. Rossnagel, and G.J.
Scoles, 2007.** Molecular marker development and linkage analysis in
three low phytic acid barley (*Hordeum vulgare*) mutant lines. Mol.
Breed. 20: 323-330.

**Varshney, R.K., T.C. Marcel, L. Ramsay, J. Russell, M.S. Roder, N.
Stein, R. Waugh, P. Langridge, R.E. Niks, and A. Graner, 2007.** A high
density barley microsatellite consensus map with 775 SSR loci. Theor.
Appl. Genet. 114: 1091-1103.

**Coordinator's report: Chromosome 2H (2)**

**J.D. Franckowiak**

**Hermitage Research Station**

**Queensland Department of Primary Industries and Fisheries**

**Warwick, Queensland 4370, Australia**

**e-mail: [jerome.franckowiak@dpi.qld.gpv.au]{.underline}**

The flowering locus T (FT) gene was first identified in Arabidopsis as
having a role in the photoperiod and vernalization responses. Members of
this family of genes, characterized by a
phosphatidylethanolamine-binding protein (PEBP) domain, were mapped in
barley by Faure *et* *al*. (2007). The family contains five members in
barley and the *HvFT4* gene mapped to the centromeric region of
chromosome 2H.

Nduulu *et al*. (2007) examined a region of chromosome 2(2H) designated
Qrgz-2H-8 in which coincident QTLs for Fusarium head blight (FHB)
severity, deoxynivalenol (DON) concentration, and heading date (HD) have
been mapped. It was unclear if FHB resistance at this locus is caused by
a pleiotropic effect of delayed heading or tightly linked genes. Nduulu
*et al*. (2007) identified a recombinant that showed reduced FHB
severity and early heading and concluded that the relationship between
FHB and HD at the Qrgz-2H-8 region is likely due to tight linkage rather
than pleiotropy. This region of 2H is the same region where the *HvFT4*
gene was mapped and maturity factors early maturity 6 (*Eam6*)
(Franckowiak 2007) and earliness per se QTL 2S (*eps2S*) Laurie *et al*.
(1995) were located.

Burton *et al*. (2008) have mapped four cellulose synthase-like *CslF*
(*HvCslF*) genes to a single locus on barley chromosome 2H. This region
corresponds to a major quantitative trait locus for grain
(1,3;1,4)-*β*-D-glucan content. Only two of the seven *CslF* genes,
*HvCslF6* (7H) and *HvCslF9* (1H), are transcribed at high levels in
developing grain and are of potential relevance for the future
manipulation of grain (1,3;1,4)-*β*-D-glucan levels.

Pourkheirandish and Komatsuda (2007) developed further the evolutionary
implications in barley of their research on cloning of alleles at the
*vrs1* (six-rowed spike 1) locus (Komatsuda *et* *al*. 2007). Six-rowed
barley arose three times as independent events from two-rowed barley,
but the oldest group of six-rowed barleys is apparently older than
existing two-rowed groups.

Forster *et al*. (2007) extended the barley phytomer concept based on
various morphological mutants observed in the Optic TILLING population
and older collections of barley mutants. Phytomers are repeated building
blocks that form various parts of the barley plant. The basic phytomer
is composed of two half nodes separated by an internode with a side arm
arising from the upper half node and root initials and a bud developing
from the lower half node. An array of development patterns modified the
basic phytomer to form various vegetative and reproductive organs. Some
genes on chromosome 2H that affect the development of morphological
structures include *abr1* (accordion basal rachis 1), *acr1* (accordion
rachis 1), *com2* (compositum 2), *eog1* (elongated outer glume 1),
*ert-t* (erectoides-t), *lig1* (liguleless 1), *Lks1* (awnless 1),
*mnd1* (many noded dwarf 1), *sbk1* (subjacent hood 1), *vrs1*
(six-rowed spike 1), and *Zeo1* (zeocriton 1).

Jafary *et al*. (2008) reported that barley germplasm contains partial
resistance QTLs to several leaf rust , caused by *Puccinia* species, for
which barley is not a host. Likewise, a population segregating for the
*Rph7* (resistance to *Puccinia hordei* 7) showed partial resistance in
the susceptible portion of the population. Several of partial resistance
QTLs for reaction leaf rust, including *Rphq2* and *Rphq6* for *P.
hordei* reaction, were located on chromosome 2H.

Sameri and Komatsuda (2007) associated a QTL for 100-kernel weight and
several other QTLs for agronomic traits with chromosome 2H.

**References:**

**Burton, R.A., S.A. Jobling, A.J. Harvey, N.J. Shirley, D.E. Mather,**
**A. Bacic, and G.B. Fincher. 2008.** The genetics and transcriptional
profiles of the cellulose synthase-like *HvCslF* gene family in barley.
*Plant Physiology* 146:1821-1833.

**Faure, S., J. Higgins, A. Turner, and D.A. Laurie. 2007.** The
*FLOWERING LOCUS T*-like gene family in barley (*Hordeum vulgare*).
Genetics 176:599-609.

**Forster, B.P., J.D. Franckowiak, U. Lundqvist, J. Lyon, I. Pitkethly,
W.T.B. Thomas. 2007.** The barley phytomer. Annals of Botany
100:725-733.

**Franckowiak, J.D. 2007.** BGS 98, early maturity 6, *Eam6*, revised.
Barley Genet. Newsl. **37**: 216−217.

**Jafary, H., G. Albertazzi, T.C. Marcel, and R.E. Niks. 2008**. High
diversity of genes for nonhost resistance of barley to heterologous rust
fungi. Genetics 178(4):2327-2339.

**Komatsuda, T., M. Pourkheirandish, C. He, P. Azhaguvel, H. Kanamori,
D. Perovic, N. Stein, A. Graner, T. Wicker, A. Tagiri, U. Lundqvist, T.
Fujimura, M. Matsuoka, T. Matsumoto, and M. Yano. 2007.** Six-rowed
barley originated from a mutation in a homeodomain-leucine zipper
I-class homeobox gene. PNAS 104:1424-1429.

**Laurie, D.A, Pratchett, N., Bezant, J.H., and Snape, J.W. 1995.** RFLP
mapping of five major genes and eight quantitative trait loci
controlling flowering time in a winter/spring barley cross. Genome
**38**: 575−585.

**Nduulu, L.M., A. Mesfin, G.J. Muehlbauer, and K.P. Smith. 2007.**
Analysis of the chromosome 2(2H) region of barley associated with the
correlated traits Fusarium head blight resistance and heading date.
Theor Appl Genet 115:561--570.

**Pourkheirandish, M., and T. Komatsuda. 2007.** The importance of
barley genetics and domestication in a global perspective. Annals of
Botany 100:999-1008.

**Sameri, M. and T. Komatsuda. 2007.** Localization of quantitative
trait loci for yield components in a cross oriental × occidental barley
cultivar (*Hordeum vulgare* L.). JARQ 41:195-199.

**Coordinator's Report: Barley Chromosome 3H**

**L. Ramsay**

**Genetics Programme**

**Scottish Crop Research Institute**

**Invergowrie, Dundee, DD2 5DA, Scotland, UK.**

**e-mail: [Luke.Ramsay@scri.ac.uk]{.underline}**

Over the last year there have been a number of publications reporting
the mapping of genes and QTL on barley chromosome 3H. One highlight was
the mapping of over 2000 Transcript Derived Markers (including 302 on
3H) in the Steptoe x Morex DH population using a genetical genomics
approach (Potokina *et al.* 2008). Using the derived map this study
found 23,738 eQTL affecting the expression of 12,987 genes with both
*cis* and *trans* effects in evidence.

Hu and Wise (2008) reported the mapping of two *Lrk/Tak* kinase gene
clusters 3HS near the telomere using the Steptoe x Morex minimapper set.
Microarray analysis revealed cultivar specific transcript accumulation
of some of the family members on 3H, which the authors interpreted as
indicating subfunctionalization of *Lrk/Tak* members following tandem
duplication. At the other end of the chromosome Tyrka *et al.* (2008)
developed a new diagnostic SSR for the *Hv-eIF4E g*ene underlying
*Rym4/Rym5* locus on 3HL. Lee and Neate (2007) mapped a single dominant
gene denoted *Rsp1* that confers resistance to Septoria spleckle leaf
blotch at seedling and adult stages to 3HS using an F~2:3~ population
derived from a Robust x CIho 14300 cross. Somewhat confusingly Yan and
Chen (2007) mapped a recessive gene denoted *rps1.a*, for resistance to
stripe rust to 3HL using RILs derived from BBA 2890 x Steptoe.

Other genes mapped included a barley haze active protein (Robinson *et
al*. 2007) on 3HS mapped using antisera screened by immunoblot on the
Chebec x Harrington DH population. Suprunova *et al*. (2007) mapped
*Hsdr4* (*Hordeum spontaneum dehydration-responsive 4*) to 3HL between
the SSR markers EBmac541 and EBmag705, using the population MA10-30 x
WQ23-38, to a region that previously had been shown to affect osmotic
adaptation in barley.

Several new reports of QTL on 3H were published during this reporting
period including Fox *et al.* (2007) a grain hardness QTL on the distal
end of 3HL using a population derived from a Patty x Tallon cross.
Munoz-Amatriain *et al.* (2008) report a QTL for green plant percentage
on anther culture that maps close to the SSR HVM60 in a cross between
Igri and an albino producing line (DH46) selected from an Igri x Dobla
cross. A major QTL for Russian Wheat aphid resistance major was mapped
to 3H (in the region of EBmac541) using 191 F~2~ derived F~3~ families
from the cross \'Morex\'/STARS-9301B QTL Morex x STARS-9301B (Mittal *et
al.* 2008). Li *et al.* (2008) mapped a waterlogging tolerance QTL using
two populations (TX9425 x Franklin and Yerong x Franklin) that showed
consistent QTL for leaf chlorosis near the centromeric region of the
genetic map of 3H. Ullrich *et al*. (2008) mapped co-incident major
preharvest sprouting and dormancy QTL in the Steptoe x Morex population
including one in the centromeric region of 3H. The same region was the
site of a minor QTL for dormancy in a Stirling x Harrington population
(Bonnardeaeux *et al*. 2008).

Using an association genetics approach Comadran *et al.* (2008) found
consistent QTL for yield in droughted environments in bin 4 on 3H. A
similar region was found associated with adaptation to Mediterranean
dryland conditions by von Korff *et al.* (2008) using RILs derived from
the ER/Apm x Tadmor cross. QTL on 3H were found for several traits
including days to heading, plant height and grain yield (von Korff *et
al.* 2008).

**References:**

**Bonnardeaux, Y., C. Li, R. Lance, X.Q. Zhang, K. Sivasithamparam, and
R. Appels, 2008.** Seed dormancy in barley: identifying superior
genotypes through incorporating epistatic interactions. Australian
Journal of Agricultural Research 59: 517-526.

**Comadran, J., J.R. Russell, F.A. van Eeuwijk, S. Ceccarelli, S.
Grando, M. Baum, A.M. Stanca, N. Pecchioni, A.M. Mastrangelo, T. Akar,
A. Al-Yassin, A. Benbelkacem, W. Choumane, H. Ouabbou, R. Dahan, J.
Bort, J.L. Araus, A. Pswarayi, I. Romagosa, C.A. Hackett, and W.T.B.
Thomas, 2008**. Mapping adaptation of barley to droughted environments.
Euphytica 161: 35-45.

**Fox, G.P., B. Osborne, J. Bowman, A. Kelly, M. Cakir, D. Poulsen, A.
Inkerman, and R. Henry, 2007.** Measurement of genetic and environmental
variation in barley (*Hordeum vulgare*) grain hardness. Journal of
Cereal Science 46: 82-92.

**Hu, P.S. and R.P. Wise, 2008.** Diversification of Lrk/Tak kinase gene
clusters is associated with subfunctionalization and cultivar-specific
transcript accumulation in barley. Functional & Integrative Genomics 8:
199-209.

**Lee, S.H. and S.M. Neate, 2007.** Molecular mapping of *Rsp1*, *Rsp2*,
and *Rsp3* genes conferring resistance to septoria speckled leaf blotch
in barley. Phytopathology 97: 155-161.

**Li, H., R. Vaillancourt, N. Mendham, and M. Zhou, 2008.** Comparative
mapping of quantitative trait loci associated with waterlogging
tolerance in barley (*Hordeum vulgare* L.). BMC Genomics 9: 401.

**Mittal, S., L.S. Dahleen, and D. Mornhinweg, 2008.** Locations of
quantitative trait loci conferring Russian wheat aphid resistance in
barley germplasm STARS-9301B. Crop Science 48: 1452-1458.

**Munoz-Amatriain, M., A.M. Castillo, X.W. Chen, L. Cistue, and M.P.
Valles, 2008.** Identification and validation of QTLs for green plant
percentage in barley (*Hordeum vulgare* L.) anther culture. Molecular
Breeding 22: 119-129.

**Potokina, E., A. Druka, Z.W. Luo, R. Wise, R. Waugh, and M. Kearsey,
2008**. Gene expression quantitative trait locus analysis of 16,000
barley genes reveals a complex pattern of genome-wide transcriptional
regulation. Plant Journal 53: 90-101.

**Robinson, L.H., P. Healy, D.C. Stewart, J.K. Eglinton, C.M. Ford, and
D.E. Evans, 2007.** The identification of a barley haze active protein
that influences beer haze stability: The genetic basis of a barley malt
haze active protein. Journal of Cereal Science 45: 335-342.

**Suprunova, T., T. Krugman, A. Distelfeld, T. Fahima, E. Nevo, and A.
Korol, 2007.** Identification of a novel gene (*Hsdr4*) involved in
water-stress tolerance in wild barley. Plant Molecular Biology 64:
17-34.

**Tyrka, M., D. Perovic, A. Wardynska, and F. Ordon, 2008**. A new
diagnostic SSR marker for selection of the Rym4/Rym5 locus in barley
breeding. Journal of Applied Genetics 49: 127-134.

**Ullrich, S.E., J.A. Clancy, I.A. del Blanco, H. Lee, V.A. Jitkov, F.
Han, A. Kleinhofs, and K. Matsui, 2008**. Genetic analysis of preharvest
sprouting in a six-row barley cross. Molecular Breeding 21: 249-259.

**von Korff, M., S. Grando, A. Del Greco, D. This, M. Baum, and S.
Ceccarelli, 2008.** Quantitative trait loci associated with adaptation
to Mediterranean dryland conditions in barley. Theoretical and Applied
Genetics 117: 653-669.

**Yan, G.P. and X.M. Chen, 2007.** Molecular mapping of the *rps1.a*
recessive gene for resistance to stripe rust in BBA 2890 barley.
Phytopathology 97 668-673.

**Coordinator's Report: Chromosome 4H.**

**Arnis Druka**

**Genetics Programme**

**Scottish Crop Research Institute**

> **Invergowrie, Dundee, DD2 5DA, Scotland, UK.**

**e-mail: <Arnis.Druka@scri.sari.ac.uk>**

Several papers that mention genes and QTLs specifically on chromosome 4H
have been published in 2007 - 2008.

Schmalenbach *et al*., 2008 report development of a set of 59 spring
barley introgression lines (ILs) from the advanced backcross population
S42. The ILs were generated by three rounds of backcrossing, two to four
subsequent selfings, and, in parallel, marker-assisted selection. Each
line includes a single marker-defined chromosomal segment of the wild
barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum), whereas the
remaining part of the genome is derived from the elite barley cultivar
Scarlett (H. vulgare ssp. vulgare). Based on a map containing 98 SSR
markers, the IL set covers so far 86.6% (1041.5 cM) of the donor genome.
Each single line contains an average exotic introgression of 39.2 cM,
representing 3.2% of the exotic genome. The set was used to map QTLs
controlling resistance to powdery mildew (Blumeria graminis f. sp.
hordei L.) and leaf rust (Puccinia hordei L.). The strongest favorable
effects were mapped to regions 1H, 0-85 cM and 4H, 125-170 cM, where
susceptibility to powdery mildew and leaf rust was decreased by 66.1 and
34.7%, respectively, compared to the recurrent parent.

Grewal *et al*., 2007 described mapping of quantitative trait loci (QTL)
associated with net blotch resistance in a doubled-haploid (DH) barley
population using diversity arrays technology (DArT) markers. One hundred
and fifty DH lines from the cross CDC Dolly (susceptible)/TR251
(resistant) were screened as seedlings in controlled environments with
net-form net blotch (NFNB) isolates WRS858 and WRS1607 and spot-form net
blotch (SFNB) isolate WRS857. The population was also screened at the
adult-plant stage for NFNB resistance in the field in 2005 and 2006. A
high-density genetic linkage map of 90 DH lines was constructed using
457 DArT and 11 SSR markers. A seedling resistance QTL (QRpts4) for the
SFNB isolate WRS857 was detected on chromosome 4H. Three QTL (QRpt6,
QRpts4, QRpt7) were associated with resistance to both net blotch forms
and lines with one or more of these demonstrated improved resistance.
Simple sequence repeat (SSR) markers tightly linked to QRpt6 and QRpts4
were identified and validated in an unrelated barley population.

Bilgic *et al*., 2007 report use of the Affymetrix Barley1 GeneChip for
comparative transcript analysis of the barley cultivar Betzes, the wheat
cultivar Chinese Spring, and Chinese Spring - Betzes ditelosomic
chromosome addition lines to physically map 1257 barley genes to their
respective chromosome arm locations. The genes were validated through
comparison with our previous chromosome-based physical mapping,
comparative in silico mapping with rice and wheat, and single feature
polymorphism (SFP) analysis. It was found to be consistent with previous
physical mapping to whole chromosomes. In silico comparative mapping of
barley genes assigned to chromosome arms revealed that the average
genomic synteny to wheat and rice chromosome arms was 63.2% and 65.5%,
respectively. In the 1257 mapped genes, 924 SFPs were identified. A
single small rearrangement event between rice chromosome 9 and barley
chromosome 4H that accounts for the loss of synteny for several genes
was also identified.

**References:**

> **Schmalenbach I., N. Körber, and K. Pillen. 2008.** Selecting a set
> of wild barley introgression lines and verification of QTL effects for
> resistance to powdery mildew and leaf rust. Theor Appl Genet.
> 117(7):1093-106.
>
> **Grewal T.S., B.G. Rossnagel, C.J. Pozniak, and G.J. Scoles. 2008.**
> Mapping quantitative trait loci associated with barley net blotch
> resistance. Theor Appl Genet. Feb;116(4):529-39.
>
> **Bilgic H., S. Cho, D.F. Garvin, and G.J. Muehlbauer. 2007.** Mapping
> barley genes to chromosome arms by transcript profiling of
> wheat-barley ditelosomic chromosome addition lines. Genome.
> 50(10):898-906.

**Chromosome 5H (7)**

No report received.

**Coordinator's Report: Chromosome 6H**

**Victoria Carollo Blake**

**Montana State University**

**Bozeman, MT 59717 USA**

**e-mail: [vblake@montana.edu]{.underline}**

The last chromosome 6H report was submitted in 1999
(<http://wheat.pw.usda.gov/ggpages/bgn/29/c29-08.html>). Since then
several genes have been characterized and QTL placed on 6H. This report
will survey the 6H genes from Andy Kleinhofs "Integrating Molecular and
Morphological/Physiological Marker Maps" and attempt to report on any
significant mapping progress made since 2000.

*Nar1* (NADH nitrate reductase) is included in 13 GrainGenes maps, with
the RFLP MWG633/cMWG633 the closest molecular marker on 'Barley,
Consensus 2005, SNP' (GrainGenes Map_Data record name) (Rostoks *et
al.*, 2005) and 'Barley, Consensus 2006, Marcel' (GrainGenes Map_Data
record name) (Marcel *et al.*, 2007). Several studies have related this
gene to nitrate assimilation and most recently, Sicher and Bunce (2008)
found barley (cv. Steptoe) with a mutant *nar1* gene (90% lower
expression) showed an elimination of the increase of glutamine,
aspartate and alanine during the latter half of a photoperiod.

*Rrs13*, conferring resistance to *Rhynchosporium secalis* (leaf blotch,
scald) is a member of a gene cluster on the short arm of 6H. A review by
Zhan *et al.*, 2008, explores gene-mediated resistance, disease
epidemiology, describes sources of resistance in barley and places 10
disease-resistance QTL onto 6HS. Grewel *et al.* (2008) describe a major
QTL, designated *QRpt6* on 6H for net blotch resistance. In a Rika x
Kombar DH population Abu-Qamar *et al*., 2008 showed segregation for at
least two major recessive resistance genes, differing in resistance to
different pathotypes of *Pyrenophora teres* f. *tere.* The NTNB
resistance loci, named *rpt.r* and *rpt.k* mapped 1.8 cM apart and were
flanked by the CAP marker ABC02895 and the locus detected by STS markers
GBS0468 and ABC01797. In 2003 Le Gouis *et al*. characterized a gene for
resistance to soil-borne barley mild mosaic virus (BaMMV) from the
cultivar Chikurin Ibaraki, *rym15*, which mapped to 6H flanked by
Bmag0173 and EBmac0874.

*sex1*, the shrunken endosperm xenia1 gene conferring high lysine was
mapped between microsatellite markers GBM5012 and GBM1063 in a 4.2 cM
interval near the centromere by Röder *et al.*, (2006). The authors
found an orthologous site on the rice chromosome 2 where the interval
between regions with homology to the barley markers spans 4.1 Mb.

*cul2*, the *uniculm2* mutation that causes plants to initiate
vegetative axillary meristems but fail to develop tillers, and alters
inflorescence morphology was genetically and morphologically
characterized by Babb and Muehlbauer in 2003. They found that *cul2* was
epistatic to all other genes in this study that influence tillering.
Linkage analysis placed *cul2* between cMWG679/ABG458 (8.8 cM) and
KFP128 (4.6 cM).

*Amy1*, the gene for α-amylase in barley is included on 24 maps
currently in GrainGenes, and is included in QTL for α-amylase activity
in Chebec x Harrington and Harrington x TR306 (Coventry *et al.*, 2003).
Suzuki *et al.,* in 2005 showed that Gibberellin (GA) biosynthesis in
the epithelium of germinating seeds is important for α-amylase
expression and cloned *HvGA3ox2*, which encodes the key enzyme. Another
region on 6H important for malt quality is a QTL for grain protein
content (Qgpc6H). This was first described by See *et al.* in 2002 who
mapped the low protein gene contributed by the cultivar 'Karl' (CIho
15487) on the 'satellite' of 6H near the anchor markers abg458, hvm74
and mwg2029. Work on this region by Distelfeld *et al.* in 2008 found
colinearity between the barley grain protein content QTL and the wheat
*Gpc-B1* region and suggested that the barley NAC transcription factor
is responsible for the protein content trait.

Perovic *et al*. (2007) mapped nine members of a multigene family for
nicotianamine synthase (NAS) in barley, three of which fell on 6H.
Nicotianamine works as a chelator for iron and other heavy metals.
Co-linearity with rice suggests that this gene went through at least one
duplication event prior to the divergence of barley and rice.

**References:**

**Abu Qamar, M., Z.H. Liu, J.D. Faris, S. Chao, M.C. Edwards, Z. Lai,
J.D. Franckowiak, and T.L. Friesen. 2008.** A region of the barley
chromosome 6H harbors multiple major genes associated with net type net
blotch resistance. Theor Appl Gen. In press.

**Babb, S. and G.J. Muehlbauer. 2003.** Genetic and morphological
characterization of the barley *uniculm2* (*cul2*) mutant. Theor. Appl.
Gen. 106:846-857.

**Coventry, S.J., H.M. Collins, A.M. Barr, S.P. Jefferies, K.J.
Chalmers, S.J: Logue, and P. Langridge. 2003.** Use of putative QTLs and
structural genes in marker assisted selection for diastatic power in
malting barley (*Hordeum vulgare* L.) Aust. J. Agr. Res. 54:1241-1250.

**Distelfeld, A., A. Korol, J. Dubcovsky, C. Uauy, T. Blake, and T.
Fahima. 2008.** Colinearity between the barley grain protein content
(GPC) QTL on chromosome arm 6HS and the wheat *Gpc-B1* region. Mol.
Breed. 22:25-38.

**Grewal, T.S., B.G. Rossnagel, C.J. Pozniak, and G.J. Scoles. 2007.**
Mapping quantitative trait loci associated with barley net blotch
resistance. Theor Appl Gen 116: 529-539.

**Le Gouis, J., P. Devaux, K. Werner, D. Hariri, N. Bahrman, D. Béghin,
and F. Ordon 2004.** *rym15*from the Japanese cultivar Chikurin Ibaraki
1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on
chromosome 6H. Theor. Appl. Genet. 108:1521-1525.

**Marcel, T.C., R.K. Varshney, M. Barbieri, H. Jafary, M.J.D. de Kock,
A. Graner, and R.E. Niks. 2007.** A high-density consensus map of barley
to compare the distribution of QTLs for partial resistance to *Puccinia
hordei* and of defence gene homologues. Theor. Appl. Genet. 114-487-500.

**Perovic, D., P. Tiffin, D. Douchkov, H. Bäumlein, and A. Graner.
2007.** An integrated approach for the comparative analysis of a
multigene family: The nicotianamine synthase genes of barley. Funct.
Int. Gen. 7:169-179.

**Röder, M.S., C. Kaiser, and W. Weschke. 2006.** Molecular mapping of
the shrunken endosperm genes *seg2* and *sex1* in barley (*Hordeum
vulgare* L.) Genome 49:1209-1214.

**Rostoks, N., S. Mudie, L. Cardle, J. Russell, L. Ramsay, A. Booth,
J.T. Svensson, S.I. Wanamaker, H. Walia, E.M. Rodriguez, P.E. Hedley, H.
Liu, J. Morris, T.J. Close, D.F. Marshall, and R.F. Waugh. 2005.**
Genome-wide SNP discovery and linkage analysis in barley based on genes
responsive to abiotic stress. Mol Genet Genomics 274:515-527.

**See, D., V. Kanazin, K. Kephart, and T. Blake. 2002.** Mapping genes
controlling variation in barley grain protein concentration. Crop Sci..
42:680-685.

**Sicher, R.C. and J.A. Bunce. 2008.** Growth, photosynthesis, nitrogen
partitioning and responses to CO~2~ enrichment in a barley mutant
lacking NADH-dependent nitrate reductase activity. Physol. Plant.
134:31-40.

**Suzuki, H., K. Ishiyama, M. Kobayashi, and T. Ogawa. 2005.** Specific
expression of the gibberellin 3ß-hydroxylase gene, *HvGA3ox2*, in the
epithelium is important for *Amy1* expression in germinating barley
seeds. Plant Biotech 22:195-200.

**von Korff, M., H. Wang, J. Léon, and K. Pillen. 2008.** AB-QTL
analysis in spring barley: III. Identification of exotic alleles for the
improvement of malting quality in spring barley (*H. vulgare* ssp.
*Spontaneum*) Mol. Breed. 21:81-93.

**Werner, K., W. Friedt, and F. Ordon. 2007.** Localisation and
combination of resistance genes against soil-borne viruses of barley
(BaMMV, BaYMV) using doubled haploids and molecular markers. Euphytica
158:323-329.

**Zhan, J., B.D.L. Fitt, H.O. Pinnschmidt, S.J.P. Oxley, and A.C.
Newton. 2008.** Resistance, epidemiology and sustainable management of
*Rhynchosporium secalis* populations on barley. Plant Phys. 57:1-14.

**Coordinator's Report: Chromosome 7H**

**Lynn S. Dahleen**

**USDA-Agricultural Research Service**

**Fargo, ND 58105, USA**

**e-mail:** **[Lynn.dahleen@ars.usda.gov]{.underline}**

Research on mapping markers and genes continued at a rapid pace in 2007.
Five high density marker maps were published along with detailed marker
information. Varshney et al. (2007) developed a consensus simple
sequence repeat (SSR) map using six mapping populations. The chromosome
7H map contained 127 markers in 157.1 cM for a marker density of 1.24.
The supplementary information provided with the paper gives primer
sequences and protocols for most of the markers on the map. Hearnden *et
al* (2007) created a map from a cross between cultivated and wild barley
(*Hordeum vulgare* ssp. *spontaneum*) using 1000 SSR and DArT markers.
They mapped 164 markers to chromosome 7H including 82 SSRs. The two
largest marker gaps also were on chromosome 7H. The third consensus map
(Wenzl *et al*. 2007) combined DArT, SSR, RFLP and STS markers and data
from ten mapping populations. The map contained 501 markers on
chromosome 7H, including 373 DArT markers. The map created by Stein et
al. (2007) combined EST-based markers and anchor markers and data from
three mapping populations. The integrated map contained 165 loci on
chromosome 7H. The fifth high density map published in 2007 was created
by Marcel *et al*. (2007) and included five mapping populations.
Approximately 50 loci were located on chromosome 7H. This map was used
to locate QTL for partial resistance to leaf rust. They show three *Rph*
loci on this chromosome, two for seedling resistance and one for adult
plant resistance.

Adult plant resistance to the net form of net blotch was mapped by
Lehmensiek *et al*. (2007) in three Australian barley populations. They
found two QTL, one on each end of chromosome 7H, that had rather small
effects compared to loci on other chromosomes. Shtaya *et al*. (2007)
examined leaf rust and powdery mildew resistance in 23 recombinant lines
containing sections of *H. bulbosum* chromosomes. Seven of the lines
contained *H. bulbosum* regions introgressed into chromosome 7H. Several
of these lines showed resistance to races of one or both of the
pathogens.

Seed traits were examined in three studies. Hori et al. (2007) located
QTL for seed dormancy in eight segregating populations. Two loci were
located on chromosome 7H, one near the centromere and one on the long
arm. In the second QTL, greater dormancy was contributed by the
non-dormant parent. Ullrich *et al*. (2008) confirmed a QTL previously
identified for dormancy and alpha-amylase activity on chromosome 7H that
was also associated with preharvest sprouting. Fox et al. (2007) located
regions associated with grain hardness using multiple methods. They
found four loci on chromosome 7H, with all loci detected by at least two
methods.

Malting quality QTL were located in two populations by Panozzo et al.
(2007) to identify linked markers for selection. They found QTL for hot
water extract, diastatic power, alpha-amylase, protein, viscosity,
beta-glucans and free alpha-amino acid content on chromosome 7H. von
Korff *et al*. (2008) also looked a malting quality traits in an
advanced backcross population from a cross between cultivated and wild
barley. They located a QTL for fine-grind extract and for Hartong 45°C
on chromosome 7H plus three QTL for friability. Some of the favorable
alleles were from the *H. spontaneum* parent.

The *FLOWERING LOCUS T*-line gene family was mapped in barley by Faure
*et al*. (2007). This family consists of five genes, including one,
*HvFT1*, which was located on the short arm of chromosome 7H. Four genes
involved in root hair formation were mapped by Janiak and Szarejko
(2007). The locus responsible for the lack of root hairs *rhl1* was
located on the short arm of chromosome 7H, proximal to the centromere.

Bilgic *et al*. (2007) used the wheat-barley ditelosomic addition lines
on the Affymetrix Barley1 GeneChip to physically map barley genes to
chromosome arms. Out of the 1257 genes located to chromosome arms, 119
were mapped to chromosome 7HS and 131 to 7HL. Of these, 60-63.8% showed
synteny with wheat. Chromosome arm 7HS was syntenic to rice chromosomes
6S and 8L and the long arm was syntenic to rice chromosomes 6L and 8S.
Single feature polymorphisms were detected in 65-79% of the chromosome
7H transcripts.

**References:**

**Bilgic, H., S. Cho, D.F. Garvin, and G.J. Muehlbauer. 2007.** Mapping
barley genes to chromosome arms by transcript profiling of wheat-barley
ditelosomic chromosome addition lines. Genome 50:898-906.

**Faure, S., J. Higgins, A. Turner, and D.A. Laurie. 2007.** The
*FLOWERING LOCUS T*-like gene family in barley (*Hordeum vulgare*).
Genetics 176:599-609.

**Fox, G.P., B. Osborne, J. Bowman, A. Kelly, M. Cakir, D. Poulsen, A.
Inkerman, and R. Henry. 2007.** Measurement of genetic and environmental
variation in barley (*Hordeum vulgare*) grain hardness. J. Cereal Sci.
46:82-92.

**Hearnden, P.R., P.J. Eckermann, G.L. McMichael, M.J. Hayden, J.K.
Eglinton, and K.J. Chalmers. 2007.** A genetic map of 1,000 SSR and DArT
markers in a wide barley cross. Theor. Appl. Genet. 115:383-391.

**Hori, K., K. Sato, and K. Takeda. 2007.** Detection of seed dormancy
QTL in multiple mapping populations derived from crosses involving novel
barley germplasm. Theor. Appl. Genet. 115:869-876.

**Janiak, A. and I. Szarejko. 2007**. Molecular mapping of genes
involved in root hair formation in barley. Euphytica 157:95-111.

**Korff, M. von, H. Wang, J. Leon, and K. Pillen. 2008.** AB-QTL
analysis in spring barley: III. Identification of exotic alleles for the
improvement of malting quality in spring barley (*H. vulgare* ssp.
*spontaneum*. Mol. Breeding 21:81-93.

**Lehmensiek, A., G.J. Platz, E. Mace, D. Poulsen, and M.W. Sutherland.
2007.** Mapping of adult plant resistance to net form of net blotch in
three Australian barley populations. Australian J. Agric. Res.
58:1191-1197.

**Marcel, T.C.. R.K. Varshney, M. Barbieri, H. Jafary, M.J.D. de Kock,
A. Graner, and R.E. Niks. 2007.** A high-density consensus map of barley
to compare the distribution of QTLs for partial resistance to *Puccinia
hordei* and of defence gene homologues. Theor. Appl. Genet. 114:487-500.

**Panozzo, J.F., P.J. Eckermann, D.E. Mather, D.B. Moody, C.K. Black,
H.M. Collins, A.R. Barr, P. Lim, and B.R. Cullis. 2007.** QTL analysis
of malting quality traits in two barley populations. Australian J.
Agric. Res. 58:858-866.

**Shtaya, M.J.Y., J.C. Sillero, K. Flath, R. Pickering, and D. Rubiales.
2007.** The resistance to leaf rust and powdery mildew of recombinant
lines of barley (*Hordeum vulgare* L.) derived from *H. vulgare* x *H.
bulbosum* crosses. Plant Breeding 126:259-267.

**Stein, N., M. Prasad, U. Scholz, T. Theil, H. Zhang, M. Wolf, R. Kota,
R.K. Varshney, D. Perovic, I. Grosse, and A. Graner. 2007.** A
1,000-loci transcript map of the barley genome: new anchoring points for
integrative grass genomics. Theor. Appl. Genet. 114:823-839.

**Ullrich, S.E., J.A. Clancy, I.A. del Blanco, H. Lee, V.A. Jitkov, F.
Han, A. Kleinhofs, and K. Matsui. 2008.** Genetic analysis of preharvest
sprouting in a six-row barley cross. Mol. Breeding 21:249-259.

**Varshney, R.K., T.C. Marcel, L. Ramsay, J. Russell, M.S. Röder, N.
Stein, R. Waugh, P. Langridge, R.E. Niks, and A. Graner. 2007.** A high
density barley microsatellite consensus map with 775 SSR loci. Theor.
Appl. Genet. 114:1091-1103.

**Wenzl, P., H. Li, J. Carling, M. Zhou, H. Raman, E. Paul, P. Hearnden,
C. Maier, L. Xia, V. Caig, J. Ovesna, M. Cakir, D. Poulsen, J. Wang, R.
Raman, K.P. Smith, G.J. Muehlbauer, K.J. Chalmers, A. Kleinhofs, E.
Huttner, and A. Kilian. 2007.** A high-density consensus map of barley
linking DArT markers to SSR, RFLP and STS loci and agricultural traits.
BMC Genomics 7:206. doi:10.1186/1471-2164-7-206.

**Integration of molecular and morphological marker maps.**

No report received.

Barley Genetics Stock Center

**Harold Bockelman**

**USDA-ARS**

**National Small Grains Germplasm Research Facility**

**1691 S, 2700 W.**

**Aberdeen, ID 83210, USA**

**e-mail: [nsgchb@ars-grin.gov]{.underline}**

Recent Additions to the Barley Genetic Stock Collection in the USDA-ARS
National Small Grains Collection.

In the past year a total of 166 accessions were added to the collection
with accession numbers GSHO 3435 to 3600, shown in Table 1. Descriptions
of these accessions are available on the GRIN database:
<http://www.ars-grin.gov/npgs>.

**Table 1. Barley Genetic Stock Collection Additions in 2007-2008.**

+------+--------+-------------------------+------------+--------------+
|      | **GSHO | # Mutant type           | **Country  | # Distri     |
|      | nu     |                         | of         | ct of origin |
|      | mber** |                         | origin**   |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3435   | T1-6ai                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3436   | T1-7ao                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3437   | T2-5ah                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3438   | T2-6aq                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3439   | T2-7aj                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3440   | T3-4ae                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3441   | T3-7ax                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3442   | T3-7aaa                 | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3443   | T5-6af                  | Germany    | S            |
|      |        |                         |            | axony-Anhalt |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3444   | Mla.1.                  | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3445   | Mla.13.                 | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3446   | Mlp.                    | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3447   | mlo.5.                  | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3448   | MlL.a                   | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3449   | Mlh                     | Germany    |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3450   | Multiple dominant       | Canada     | Alberta      |
|      |        | marker stock            |            |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3451   | Multiple recessive      | Canada     | Alberta      |
|      |        | marker stock            |            |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3452   | T4-5q                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3453   | T4-5r                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3454   | T4-5s                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3455   | T4-5t                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3456   | T4-5u                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3457   | T4-5v                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3458   | T4-5w                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3459   | T4-5x                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3460   | T4-5y                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3461   | T4-5z                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3462   | T4-5aa                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3463   | T4-5ab                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3464   | T4-6a                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+

**Table 1. contin.**

+------+--------+-------------------------+------------+--------------+
|      | **GSHO | # Mutant type           | **Country  | # Distri     |
|      | nu     |                         | of         | ct of origin |
|      | mber** |                         | origin**   |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3465   | T4-6b                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3466   | T4-6c                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3467   | T4-6d                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3468   | T4-6e                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3469   | T4-6f                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3470   | T4-6g                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3471   | T4-6h                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3472   | T4-6i                   | United     | Colorado     |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3473   | T4-6j                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3474   | T4-6k                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3475   | T4-6l                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3476   | T4-6m                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3477   | T4-6n                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3478   | T4-6o                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3479   | T4-6p                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3480   | T4-6q                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3481   | T4-6r                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3482   | T4-6s                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3483   | T4-6t                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3484   | T4-6u                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3485   | T4-6v                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3486   | T4-7a                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3487   | T4-7b                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3488   | T4-7c                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3489   | T4-7d                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3490   | T4-7e                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3491   | T4-7f                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3492   | T4-7g                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3493   | T4-7h                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3494   | T4-7i                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3495   | T4-7j                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3496   | T4-7k                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3497   | T4-7l                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3498   | T4-7m                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3499   | T4-7n                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3500   | T4-7o                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3501   | T4-7p                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3502   | T4-7q                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3503   | T4-7r                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3504   | T4-7s                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3505   | T5-6a                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3506   | T5-6b                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3507   | T5-6c                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3508   | T5-6d                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3509   | T5-6e                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3510   | T5-6f                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3511   | T5-6g                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3512   | T5-6h                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3513   | T5-6i                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+

**Table 1. contin.**

+------+--------+-------------------------+------------+--------------+
|      | **GSHO | # Mutant type           | **Country  | # Distri     |
|      | nu     |                         | of         | ct of origin |
|      | mber** |                         | origin**   |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3514   | T5-6j                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3515   | T5-6k                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3516   | T5-6l                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3517   | T5-6m                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3518   | T5-6n                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3519   | T5-6o                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3520   | T5-6p                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3521   | T5-6q                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3522   | T5-6r                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3523   | T5-6s                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3524   | T5-6t                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3525   | T5-6u                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3526   | T5-6v                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3527   | T5-7a                   | United     | Minnesota    |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3528   | T5-7b                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3529   | T5-7c                   | Canada     | Manitoba     |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3530   | T5-7d                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3531   | T5-7e                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3532   | T5-7f                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3533   | T5-7g                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3534   | T5-7h                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3535   | T5-7i                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3536   | T5-7j                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3537   | T5-7k                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3538   | T5-7l                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3539   | T5-7m                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3540   | T5-7n                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3541   | T5-7o                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3542   | T5-7p                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3543   | T5-7q                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3544   | T5-7r                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3545   | T5-7s                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3546   | T5-7t                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3547   | T5-7u                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3548   | T5-7v                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3549   | T5-7w                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3550   | T5-7x                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3551   | T5-7y                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3552   | T5-7z                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3553   | T5-7aa                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3554   | T6-7a                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3555   | T6-7b                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3556   | T6-7c                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3557   | T6-7d                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3558   | T6-7e                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3559   | T6-7f                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3560   | T6-7g                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3561   | T6-7h                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3562   | T6-7i                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3563   | T6-7j                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+

**Table 1. contin.**

+------+--------+-------------------------+------------+--------------+
|      | **GSHO | # Mutant type           | **Country  | # Distri     |
|      | nu     |                         | of         | ct of origin |
|      | mber** |                         | origin**   |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3564   | T6-7k                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3565   | T6-7l                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3566   | T6-7m                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3567   | T6-7n                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3568   | T6-7o                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3569   | T6-7p                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3570   | T6-7q                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3571   | T6-7r                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3572   | T6-7s                   | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3573   | T6-7t                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3574   | T6-7u                   | Germany    | Bavaria      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3575   | T6-7v                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3576   | T6-7w                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3577   | T6-7x                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3578   | T6-7y                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3579   | T6-7z                   | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3580   | T6-7aa                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3581   | T6-7ab                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3582   | T6-7ac                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3583   | T6-7ad                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3584   | T6-7ae                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3585   | T6-7af                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3586   | T6-7ag                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3587   | T6-7ah                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3588   | T6-7ai                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3589   | T6-7aj                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3590   | T6-7ak                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3591   | T6-7al                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3592   | T6-7am                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3593   | T6-7an                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3594   | T6-7ao                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3595   | T6-7ap                  | Sweden     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3596   | T6-7aq                  | United     | Arizona      |
|      |        |                         | States     |              |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3597   | Mutant 1661             | Sweden     | Uppsala      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3598   | Mutant 2721             | Sweden     | Uppsala      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3599   | Mutant 3091             | Sweden     | Uppsala      |
+------+--------+-------------------------+------------+--------------+
| GSHO | 3600   | Mutant 3550             | Sweden     | Uppsala      |
+------+--------+-------------------------+------------+--------------+

**References:**

<http://ace.untamo.net/bgs>

<http://www.ars-grin.gov/npgs>

**Wright, S.A.I., M. Azarang, and A.B. Falk. 2007.** Four new barley
mutants. Barley Genetics Newsletter 37:34-36.

**Coordinator's report: Translocations and**

**balanced tertiary trisomics**

**Andreas Houben**

# Leibniz-Institute of Plant Genetics and Crop Plant Research

**DE-06466 Gatersleben, Germany**

**email: <houben@ipk-gatersleben.de>**

Prof. M. Molnar-Lang and colleagues succeeded in developing
translocation lines by inducing homologous chromosome pairing in a
4H(4D) wheat-barley substitution line previously developed in
Martonvasar (Sepsi *et al*., 2006). It was hoped to incorporate various
segments of the barley 4H chromosome from the 4H(4D) substitution into
wheat. Observations were made on the frequency with which wheat-barley
translocations appeared in the F-2 progeny grains from a cross between
the line CO4-1, which carries the Ph suppressor gene from *Aegilops
speltoides* and thus induces a high level of homologous chromosome
pairing, and the 4H(4D) wheat-barley substitution line, and on which
chromosome segments were involved in the translocations. Of the 117
plants examined, three (2.4%) were found to contain translocations. A
total of four translocations were observed, as one plant contained two
different translocations. The translocations consisted of one centric
fusion, two dicentric translocations and one acrocentric chromosome.

Prof. K. Gecheff (Institute of Genetics, Sofia, Bulgaria) kindly donated
42 homozygous single translocation lines produced by gamma-irradiation
of spring two-rowed barley variety 'Freya'. All lines are precisely
characterized with respect to the chromosomal localization of the
translocation break points (Gecheff, 1996).

The collection is being maintained in cold storage. To the best
knowledge of the coordinator, there are no new publications dealing with
balanced tertiary trisomics in barley. Limited seed samples are
available any time, and requests can be made to the coordinator.

**Reference:**

**Gecheff, K. I., 1996.** Production and identification of new
structural chromosome mutations in barley (*Hordeum vulgare* L).
Theoretical and Applied Genetics. 92:777-781

**Sepsi, A., K. Nemeth, I. Molnar, E. Szakacs, and M. Molnar-Lang.
2006.** Induction of chromosome rearrangements in a 4H(4D) wheat-barley
substitution using a wheat line containing a Ph suppressor gene. Cereal
Research Communications 34: 1215-1222    

**Trisomic and aneuploid stocks**

No report received

**Coordinator's report: Autotetraploids**

**Wolfgang Friedt, Institute of Crop Science and Plant Breeding I.**

**Justus-Liebig-University, Heinrich-Buff-Ring 26-32**

**DE-35392 Giessen, Germany**

**e-mail: <wolfgang.friedt@agrar.uni-giessen.de>**

**Fax: +49(0)641-9937429**

The collection of barley autotetraploids (exclusively spring types)
described in former issues of BGN is maintained at the Giessen Field
Experiment Station of our institute. The set of stocks, i.e.
autotetraploids (4n) and corresponding diploid (2n) progenitors (if
available) have last been grown in the field for seed multiplication in
summer 2000. Limited seed samples of the stocks are available for
distribution.

**Coordinator's report: *Eceriferum* genes**

**Udda Lundqvist**

**Nordic Genetic Resource Center**

**P.O. Box 41, SE-230 53 Alnarp, Sweden**

**e-mail: <udda@nordgen.org>**

No research work on gene localization has been reported on the
collections of *Eceriferum* and *Glossy* genes. All descriptions in
Barley Genetics Newsletter (BGN) Volume 26 are valid and still
up-to-date. Several ones are revised especially in BGN 37, All Swedish
*Eceriferum* alleles can be found in the SESTO database information
system of the Nordic Genetic Resource Center, Sweden. Descriptions,
images and graphic chromosome map displays of these genes are available
in the AceDB database for Barley Genes and Barley Genetic Stocks with
its address found by: [www.untamo.net/bgs](http://www.untamo.net/bgs) .
It gets updated continouosly and also searchable through the Triticeae
database GrainGenes.

Every research of interest in the field and literature references of
these genes can be reported to the coordinator as well. Seed requests
regarding the Swedish mutant alleles can be forwarded to the coordinator
<udda@nordgen.org> or to the Nordic Genetic Resource Center,
[www.nordgen.org/ngb](http://www.nordgen.org/ngb) , all the others to
the Small Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID
83210, USA, <nsgchb@ars-grin.gov> or to the coordinator at any time.

**Coordinator's report: Nuclear genes affecting the chloroplast**

**Mats Hansson**

**Carlsberg Laboratory,**

**Gamle Carlsberg Vej 10,**

**DK-2500 Valby,**

**Copenhagen, Denmark**

**E-mail: [mats@crc.dk]{.underline}**

Barley mutants deficient in chlorophyll biosynthesis and chloroplast
development are easily distinguished from wild type plants by their
deviant colour. Therefore, chlorophyll mutants have often been used to
optimise and calibrate mutagenesis methods (Lundqvist 1992). Chlorophyll
mutants have been named *albina*, *xantha*, *viridis*, *chlorina*,
*tigrina* and *striata* depending on their colour and colour pattern. In
the *albina* mutants the leaves are completely white due to lack of both
chlorophyll and carotene pigments. The *xantha* mutants are yellow and
produce carotene, but no chlorophyll. The *chlorina* and *viridis*
mutants are both pale green, but differ in *chlorina* being viable. The
*tigrina* and *striata* mutants are stripped transverse and along the
leaves, respectively.

Frigerio *et al*. (2007) utilized the *viridis-zb*.63 mutant to study
the transcription and accumulation of light-harvesting complexes in
barley. In *viridis-zb*.62 the photosystem I is depleted and the
plastoquinone pool is constitutively reduced. They showed that that the
mRNA level of all photosynthesis-related genes including genes encoding
antenna proteins are almost unaffected in the mutant. In contrast,
analysis of protein accumulation showed that the mutant undergoes strong
reduction of its antenna size, with individual gene products having
different levels of accumulation. They conclude that the plastoquinone
redox state plays an important role in the long term regulation of
chloroplast protein expression, but its modulation is active at the
post-transcriptional rather than transcriptional level.

Zakhrabekova *et al*. (2007) evaluated the possibility to clone genes
deficient in barley mutants by a microarray approach. In their study
barley mutants *xantha-h*.57 and *xantha-f*.27 were used in combination
with the Affymetrix microarray platform. Both *xantha-h*.57 and
*xantha-f*.27 are deficient in the chlorophyll biosynthetic enzyme
magnesium chelatase, but in different genes encoding two of the three
subunits of this very complex enzyme. Mutant *xantha-h*.57 produces no
*Xantha-h* mRNA whereas in *xantha-f*.27 the nonsense mutation in the
last exon of the gene, results in nonsense-mediated decay of *Xantha-f*
mRNA. Among the 22,792 probe sets arrayed on the Affymetrix chip, the
*Xantha-h* and *Xantha-f* genes were possible to highlight in a
competitive analysis between *xantha-h*.57 and *xantha-f*.27. It was
concluded that it should be possible to use the approach of combining
the Affymetrix platform with phenotypically similar mutants in order to
clone genes only known through their mutant phenotype.

The stock list of barley mutants defective in chlorophyll biosynthesis
and chloroplast development is found elsewhere in the issue of BGN 37
and at

http://www.mps.lu.se/fileadmin/mps/People/Hansson/Barley_mutants_web.pdf

**Lundqvist, U.** 1992. Mutation research in barley. PhD Thesis. The
Swedish University of Agricultural Sciences. Svalöv. Available from

http://www.mps.lu.se/fileadmin/mps/People/Hansson/Uddas_thesis.pdf

**New references:**

**Frigerio, S., C. Campoli, S. Zorzan, L. I. Fantoni, C. Crosatti, F.
Drepper, W. Haehnel, L. Cattivelli, T. Morosinotto and R. Bassi.** 2007.
Photosynthetic antenna size in higher plants is controlled by the
plastoquinone redox state at the post-transcriptional rather than
transcriptional level. J. Biol. Chem. 282: 29457-29469.

**Zakhrabekova, S., S. P. Gough, U. Lundqvist and M. Hansson.** 2007.
Comparing two microarray platforms for identifying mutated genes in
barley (*Hordeum vulgare* L.). Plant Physiol. Biochem. 45: 617-622.

**Coordinator's report: The Genetic Male**

**Sterile Barley Collection**

**M.C. Therrien**

**Agriculture and Agri-Food Canada**

**Brandon Research Centre**

**Box 1000A, RR#3, Brandon, MB**

**Canada R7A 5Y3**

**E-mail: [MTherrien@agr.gc.ca]{.underline}**

The GMSBC has been at Brandon since 1992. If there are any new sources
of male-sterile genes that you are aware of, please advise me, as this
would be a good time to add any new source to the collection. For a list
of the entries in the collection, simply E-mail me at the above address.
I can send the file (14Mb) in Excel format. We continue to store the
collection at -20^o^C and will have small (5 g) samples available for
the asking. Since I have not received any reports or requests the last
years, there is absolutely no summary in my report.

Coordinator's report: Early maturity and

Praematurum genes

**Udda Lundqvist**

**Nordic Genetic Resource Center**

**P-O. Box 41**

**SE-23 053 Alnarp, Sweden**

**e-mail: [udda@nordgen.org]{.underline}**

Not much new research on gene localization has been reported on the
Early maturity or Praematurum genes since the latest reports in Barley
Genetic Newsletter (BGN) or in the AceDB database for Barley Genes and
Barley Genetic Stocks.

All information and descriptions made in the Barley Genetics Newsletter
are valid and still up-to-date. Some of them are revised especially in
BGN 37. All the Swedish Praematurum genes with its alleles can be found
in the SESTO database information system of the Nordic Genetic Resource
Center, Sweden. Descriptions, images and graphic chromosome map displays
of these early maturity or Praematurum genes are available in the AceDB
database for Barley Genes and Barley Genetic Stocks with its address
found by: [www.untamo.net/bgs](http://www.untamo.net/bgs) . It gets
updated continuously and also searchable through the Triticeae database
GrainGenes.

Every research of interest in the field and literature references of
these genes can be reported to the coordinator as well. Seed requests
regarding the Swedish mutant alleles can be forwarded to the coordinator
or directly to the Nordic Genetic Resource Center,
[www.nordgen.org/ngb]{.underline}, all others to the Small Grain
Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
<nsgchb@ars-grin.gov> or to the coordinator at any time.

**Coordinator's report: Ear morphology genes**

**Udda Lundqvist**

**Nordic Genetic Resource Center**

**P.O. Box 41, SE-230 53 Alnarp, Sweden.**

**e-mail: <udda@nordgen.org>**

Since the last report in Barley Genetics Newsletter several descriptions
on morphological ear genes have been revised and updated and one new
description on the Double seed 1 (*dub1*) gene has been performed
(Dahleen *et al*. 2007). New developmental mutants as a guide to the
barley phytomer were studied where several ear motphological genes were
included (Forster *et al*. 2007, Franckowiak *et al*. 2008). All ear
morphological genes are backcrossed to the cultivar 'Bowman' and are
available with special GSHO numbers.

All earlier descriptions in the Barley Genetics Newsletter (BGN) volumes
26, 28, 29, 32, 35 and 37 are up-to-date and valid. They are also
updated in the AceDB database for Barley Genes and Barley Genetic Stocks
and searchable with its address found by:
[www.untamo.net/bgs](http://www.untamo.net/bgs)

Every research in the field and literature references of these genes can
be reported to the coordinator as well. Seed requests regarding the
Swedish mutant alleles can be forwarded to the coordinator
<udda@nordgen.org> or to the Nordic Genetic Resource Center,
[www.nordgen.org/ngb](http://www.nordgen.org/ngb) , regarding all the
others and the Bowman near isogenic lines to the Small Grain Germplasm
Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
<nsgch@ars-grin.gov> or to the coordinator at any time.

**References:**

**Forster, B.P., J.D. Franckowiak, U. Lundqvist, J. Lyon, L. Pitkethly,
and W.T.B. Thomas. 2007.** The barley phytomer. Annals of Botany 100:
725-733.

**Franckowiak, J.D., B.P. Forster, U. Lundqvist, J. Lyon, I. Pitkethly,
and W.T.B. Thomas. 2008.** Developmental Mutants as a Guide to the
Barley Phytomer. Proc. Xth Intern. Barley Genet. Symp. 5.-10. April
2008, Alexandria Egypt. (in press).

**Coordinator's report : Wheat-barley genetic stocks**

## A.K.M.R. Islam

**Faculty of Agriculture, Food & Wine,**

**The University of Adelaide, Waite Campus,**

**Glen Osmond, SA 5064, Australia**

**e-mail: [rislam@waite.adelaide.edu.au]{.underline}**

The production of five different disomic addition lines (1Hm, 2Hm, 4Hm,
5Hm and 7Hm) of *Hordeum marinum* chromosomes to Chinese Spring wheat
has been reported earlier. It has now been possible to isolate a disomic
addition for chromosome 6Hm. Amphiploids between *H. marinum* and
commercial spring wheats have been reported earlier. Amphiploids of *H.
marinum* with winter wheats have also been produced in the mean time.
These amphiploids show better waterlogging and salt tolerance than wheat
parents (Islam and Colmer, unpublished).

**Reference:**

**Islam, A.K.M.R and T.D. Colmer. 2008.** Attempts to transfer salt-and
waterlogging tolerances from Sea barleygrass (*Hordeum marinum* Huds.)
to wheat. Proc. 11th Int. Wheat Genet. Symposium, 24-29 August 2008,
Brisbane, Australia (in Press).

**Coordinator's report: Semidwarf genes**

**J.D. Franckowiak**

**Hermitage Research Station**

**Queensland Department of Primary Industries and Fisheries**

**Warwick, Queensland 4370, Australia**

**e-mail: jerome.franckowiak@dpi.qld.gpv.au**

Using the DNA sequence of rice mutants at the gibberellin (GA)
insensitive dwarf 1 (*Gid1*) locus, a GA receptor, Chandler et al.
(2008) demonstrated that the putative orthologue from barley is the GA
sensitivity 1 (*gse1*) locus. Of 35 *gse1* mutants evaluated, 16 carried
different unique nucleotide substitution in this sequence. Study of
maximal daily elongation rate (LER~max~) of the first leaf of germinated
grains with different GA treatments revealed considerable variation in
LER~max~ values, which related closely to the degree of dwarfing
observed during plant growth. The *gse1* mutants and their GA responses
were previous described by Chandler and Robertson (1999). The *gsela*
mutant was characterized by low alpha-amylase levels, but the mutant was
re responsive to GA treatments (Chandler and Robertson, 1999). The study
of individual *gse1* mutants demonstrated some response differences
among the *gse1* mutants examined (Chandler et al., 2008).

Willige et al. (2007) reported that the DELLA domain of GA insensitive
of barley mutants at the slender 1 (*sln1*) locus suppress GA responses
These mutant genes and similar mutants of maize and wheat mutants, when
introduced into *Arabidopsis*, conferred GA insensitivity. The *sln1*
mutants in barley were previously described by Chandler et al. (2002).

**Chandler, P.M., C.A. Harding, A.R. Ashton, M.D. Mulcair, N.E. Dixon
and L.N. Mander. 2008.** Characterization of gibberellin receptor
mutants of barley (*Hordeum vulgare* L.) Molecular Plant 1:285-294.

**Chandler, P.M., A. Marion-Poll, M. Ellis and F. Gubler. 2002.**
Mutants at the *Slender1* locus of barley cv. Himalaya. Molecular and
physiological characterization. Plant Physiol. 129: 181-190.

**Chandler, PM, and M. Robertson. 1999.** Gibberellin dose--response
curves and the characterization of dwarf mutants of barley. Plant
Physiol 120:623-632.

**Willige, B.C., S. Ghosh, C. Nill, M. Zourelidou, E.M.N. Dohmann, A.
Maier and C. Schwechheimer. 2007.** The DELLA domain of GA INSENSITIVE
mediates the interaction with the GA INSENSITIVE DWARF1A gibberellin
receptor of *Arabidopsis*. Plant Cell 19:1209-1220.