**Number range CHAPTERJASON W. RAUSCH, PH.D.**

CURRICULUM VITAE

[Education]{.underline}:

1990 B.S. (Biology, minor in Mathematics) Syracuse University, Syracuse,

New York

1997 Ph.D. (Biochemistry) Case Western Reserve University, Cleveland,
Ohio

Advisor: Dr. S. F. J. Le Grice

2002 B.S. (Computer and Information Science) University of Maryland,

University College, Adelphi, Maryland

2002 Certificate (UNIX System Administration) University of Maryland,

University College, Adelphi, Maryland

2002 Certificate (Database Design and Development) University of
Maryland,

University College, Adelphi, Maryland

[Brief Chronology of Employment]{.underline}:

1990-1997 Medical Scientist Training Program Fellow, School of Medicine,
Case Western Reserve University, Cleveland, Ohio

1998-1999 Research Associate**,** Department of Medicine, Division of
Infectious Diseases, School of Medicine, Case Western Reserve
University, Cleveland, Ohio

1999-Present Staff Scientist, National Cancer Institute - Frederick, HIV
Drug Resistance Program, Frederick, Maryland

[Computer Skills:]{.underline}

-   Certified in UNIX System Administration

-   Certified in Database Design and Development

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-   Trained in the use of C, C++, and Perl computer programming
    languages

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-   Trained/Experienced in use of database, molecular modeling, and
    bioinformatics software packages including:

Oracle 8i MacVector

Swiss PDB Viewer DNA Strider

Insight II DSGene

Swiss PDB Viewer Clone Manager

DS Modeling

[Awards:]{.underline}

-   Recipient of the 2007 NCI Director's Career Development Innovation
    Award

[Committees and Boards:]{.underline}

-   Frequent Ad Hoc Reviewer for Biochemistry, Journal Biological
    Chemistry, Nucleic Acids Research, 1996-present

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-   Member, Student Advisory Committee, Medical Scientist Training
    Program, 1995-1997

[Supervisory/Teaching Experience:]{.underline}

-   1996-present: Trained and supervised undergraduate students,
    graduate students, post-doctoral fellows, and technicians of various
    levels

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-   1995-1997: Teaching assistant for graduate level course entitled
    "Physical Biochemistry"

[Meeting Abstracts:]{.underline}

Rausch, J.W., B. Wohrl, S.F.J. Le Grice. 1995. Polymerase and RNase H
activities are altered by

carboxy-terminal truncation of EIAV reverse transcriptase. Poster
presentation at the Retroviruses Meeting, Cold Spring Harbor, NY, May
23-28.

Rausch, J.W., K.J. Howard and S.F.J. Le Grice. 1997. Mutations in the
RNase H domain of retroviral RT can influence polypurine tract
selection. Poster presentation at the Palm Springs Symposium on
HIV/AIDS, Palm Springs, CA, March 13-16.

Rausch, J.W. and S.F.J. Le Grice. 1997. Converting HIV-1 reverse
transcriptase into a chemical nuclease. Poster presentation at the
Cleveland Virology Symposium, Cleveland, OH, October 27-29.

Rausch, J.W., K.J. Howard and S.F.J. Le Grice. 1999. Mutating a
conserved residue of the RNase H domain alters substrate hydrolysis by
retroviral reverse transcriptase". Poster presentation at the RNase H
Meeting, Ocean City, MD, October 7-10.

Rausch, J.W., B.K. Sathyarayana, M. Bona, and S.F.J. Le Grice. 2000.
Probing contacts between the ribonuclease H domain of HIV-1 reverse
transcripase and nucleic acid by site-specific photocross-linking. Oral
Presentation at the Retroviruses Meeting, Cold Spring Harbor, NY, May
23-28.

Rausch, J.W., D. Lener, J.T. Miller, J.G. Julias, S.H. Hughes, S.F.J. Le
Grice. 2001. Altering the RNase H primer grip of human immunodeficiency
virus reverse transcriptase modifies cleavage specificity. Poster
presentation at the NCI Symposium, Annapolis, MD, October 8-10.

Rausch, J.W., J. Qu, H.Y. Yi-Brunozzi, E.T. Kool, S.F.J. Le Grice. 2003.
Hydrolysis of RNA/DNA hybrids containing non-polar pyrimidine isosteres
defines regions essential for HIV-1 polypurine tract selection. Poster
presentation at the Retroviruses Meeting, Cold Spring Harbor, NY, May
20-25.

Rausch, J.W., J. Qu, H.Y. Yi-Brunozzi, E.T. Kool, S.F.J. Le Grice. 2003.
Hydrolysis of RNA/DNA hybrids containing non-polar pyrimidine isosteres
defines regions essential for HIV-1 polypurine tract selection. Poster
presentation at the meeting of Groups Studying the Structure of
AIDS-Related Systems, Bethesda, MD, June 18-20

Rausch, J.W. and S.F.J. Le Grice. 2006. Purine analog substitution of
the HIV-1 polypurine tract primer defines regions controlling initiation
of plus-strand DNA synthesis. Oral Presentation at the HIV Drug
Resistance Program Think Tank, Frederick, MD, April 21

Rausch, J.W. and S.F.J. Le Grice. 2006. Purine analog substitution of
the HIV-1 polypurine tract primer defines regions controlling initiation
of plus-strand DNA synthesis. Oral Presentation at the 9^th^
International Conference on RNase H, St. Michaels, MD, September 10-13

[Peer-Reviewed Publications (19):]{.underline}

Payne, S.L., J. Rausch, K. Rushlow, R.C. Montelaro, C. Issel, M.
Flaherty, S. Perry, D. Sellon, F. Fuller (1994) Characterization of
infectious molecular clones of equine infectious anaemia virus. Journal
of General Virology 75: 425-429

Cirino, N.M., C.E. Cameron, J.S. Smith, J.W. Rausch, M.J. Roth, S.J.
Benkovic, S.F.J. Le Grice (1995) Divalent cation modulation of the
ribonuclease H functions of human immunodeficiency virus reverse
transcriptase. Biochemistry 34 (31): 9936-9943

Rausch, J.W., E.J. Arts, B.M. Wohrl, S.F.J. Le Grice (1996) Involvement
of C-terminal structural elements of equine infectious anemia virus
reverse transcriptase in DNA polymerase and ribonuclease H activities.
Journal of Molecular Biology 257: 500-511

Arts, E.J., S.R. Stetor, X. Li, J.W. Rausch, K.J. Howard, B. Ehresmann,
T.W. North, B.M. Wohrl, R.S. Goody, M.A. Wainberg, S.F.J. Le Grice
(1996) Initiation of (-) strand DNA synthesis from tRNA^Lys,3^ on
lentiviral RNAs: Implications of specific HIV-1 RNA-tRNA^Lys,3^
interactions inhibiting primer utilization by retroviral reverse
transcriptases. Proc. Natl. Acad. Sci. USA 93: 10063-10068

Rausch, J.W. and S.F.J. Le Grice (1997) Substituting a conserved residue
of the ribonuclease H domain alters substrate hydrolysis by retroviral
reverse transcriptase. Journal of Biological Chemistry 272 (13):
8602-8610

Rausch, J.W. and S.F.J. Le Grice (1997) Reverse transcriptase-associated
ribonuclease H activity as a target for antiviral chemotherapy.
Antiviral Chemistry and Chemotherapy 8(3): 173-185

Stetor, S.R., J.W. Rausch, M.-J. Guo, L.R. Boone, M.J. Waring, S.F.J. Le
Grice (1999) Characterization of Plus Strand Initiation and Termination
Sequences Located at the Center of the Equine Infectious Anemia Virus
Genome. Biochemistry 38(12): 3656-67.

Cowan, J.A., T. Ohyama, K. Howard, J.W. Rausch, S.M.L. Cowan, S.F.J. Le
Grice (1999) Metal-ion stoichiometry of the HIV-RT ribonuclease H
domain. Evidence for two mutually exclusive sites leads to new
mechanistic insights on metal-mediated hydrolysis in nucleic acid
biochemistry. J Biol Inorg Chem 5(1): 67-74

Rausch, J.W., B.K. Sathyaranaya, M. Bona, S.F.J. Le Grice (1999) Probing
contacts between the ribonuclease H domain of human immunodeficiency
virus type I reverse transcriptase and nucleic acid by site-specific
photocross-linking. Journal of Biological Chemistry, 275 (21): 16015-22

Rausch, J.W., M. K. Bona-Le Grice, M. Henrietta Nymark-McMahon, Jennifer
T. Miller, and Stuart F. J. Le Grice (2000) Interaction of p55 reverse
transcriptase from the Saccharomyces cerevisiae retrotransposon Ty3 with
conformationally distinct nucleic acid duplexes. Journal of Biological
Chemistry, 275(18): 13879-87

Miller, J.T., J.W. Rausch, and S.F.J. Le Grice (2001) Evaluation of
Retroviral RNase H Activity, *In* C.H. Schein (ed.), Methods in
Molecular Biology: Nuclease Methods and Protocols 160:335-354. Humana
Press Inc., Totowa, N.J*.*

Gorshkova, I.I., J.W. Rausch, S.F.J. Le Grice, Robert J. Crouch (2001)
HIV-1 reverse transcriptase interaction with model RNA-DNA duplexes.
Analytical Biochemistry, 291(2): 198-206.

Rausch, J.W., D. Lener, J.T. Miller, J.G. Julias, S.H. Hughes, S.F.J. Le
Grice (2002) Altering the RNase H primer grip of human immunodeficiency
virus reverse transcriptase modifies cleavage specificity. Biochemistry,
41(15): 4856-4865

Cristofaro, J.V., J.W. Rausch, S.F.J. Le Grice, J.J. DeStefano (2002)
Mutations in ribonuclease H active site residues of HIV-1 reverse
transcriptase reveals magnesium-dependence of template-primer binding.
Biochemistry, 41(36): 10968-10975

Rausch, J.W., J. Qu, H.Y. Yi-Brunozzi, E.T. Kool, S.F.J. Le Grice (2003)
Hydrolysis of RNA/DNA hybrids containing non-polar pyrimidine isosteres
defines regions essential for HIV-1 polypurine tract selection.
Proceedings of the National Academy of Sciences, 2003(100): 11279-11284

Dash C., J.W. Rausch, S.F.J. Le Grice (2004) Using pyrrolo-deoxycytosine
to probe RNA/DNA hybrids containing the human immunodeficiency virus
type-1 3' polypurine tract. Nucleic Acids Research, 32(4): 1539-1547

Rausch, J.W. and S.F.J. Le Grice (2004) \'Binding, bending and
bonding\': polypurine tract-primed initiation of plus strand DNA
synthesis in human immunodeficiency virus. International Journal of
Biochemistry and Cell Biology. International Journal of Biochemistry and
Cell Biology, 36(9): 1752-1766

Rausch, J.W. and S.F.J. Le Grice (2006) Exploiting Structurally Diverse
Nucleoside Analogs as Probes of Reverse Transcription Complexes. Current
HIV Research, 5(1): 11-22

Rausch, J.W. and S.F.J. Le Grice (2007) Purine analog substitution of
the HIV-1 polypurine tract primer defines regions controlling initiation
of plus-strand DNA synthesis.\
Nucleic Acids Research, 35(1): 256-68

[Research Interests and Future Directions:]{.underline}

The principal focus of my research career has been to use various tools
of biochemistry, molecular biology, and computer modeling to understand
the interaction between/among retroviral enzymes (reverse transcriptase,
integrase) and structural proteins (nucleocapsid, *gag*) and their
nucleic acid substrates. In particular, I have developed a number of *in
vitro* assays utilizing substrates containing nucleoside analogs
(*e.g*., difluorotoluene, iso-guanosine, 2-amino-adenosine, etc.) and
purified recombinant enzymes to study how HIV-1 reverse transcripase
specifically recognizes its cognate primer for plus-strand DNA
synthesis, the HIV-1 polypurine tract (PPT). I am currently also using
related methodologies to explore the mechanism(s) by which the
ubiquitous cellular enzyme APOBEC3G, an HIV restriction factor,
catalyzes cytidine deamination within select target sequences in
single-stranded viral DNA. Finally, I have recently received a 2007 NCI
Director's Career Development Innovation Award for my application
entitled "Evolving Sequence-Specific Integrases and Methyltransferases
by *In Vitro* Compartmentalization". With these funds, I plan to use
novel directed evolution techniques to help improve the
target-specificity of retroviral gene therapy vectors, as well as tools
useful for epigenetics research pertaining to cytosine methylation.