**CURRICULUM VITAE**

**Gregory D. Wiens**

National Center for Cool and Cold Water Aquaculture

Agricultural Research Services, USDA

Kearneysville, West Virginia 25430

Phone (304) 724-8340 x 2137, E-mail greg.wiens@ars.usda.gov

**EDUCATION**

1992 Ph.D. Department of Microbiology, Oregon State University;
Corvallis, OR.

Thesis Advisor: Dr. Steve Kaattari

1983 B.A. Occidental College; Los Angeles, CA. Major: Biology

**RESEARCH EXPERIENCE**

2004-present Lead Scientist and Research Molecular Biologist, National
Center for Cool and Cold Water Aquaculture, Agricultural Research
Services, U.S. Department of Agriculture

2003-2004 Research Molecular Biologist (Immunology), NCCCWA, ARS/USDA

1998-2003 Research Assistant Professor, Department of Microbiology,
Department of Molecular Microbiology and Immunology Oregon Health
Sciences Univ.

1998. Post-doctoral Research Associate in the Laboratory of Dr. Marvin

Rittenberg; OHSU.

1992. Post-doctoral Research Associate in the Laboratory of Dr. Steve
      Kaattari;

> Department of Microbiology, Oregon State University.

1991. Graduate Research Assistant in the Laboratory of Dr. Steve
      Kaattari;

Department of Microbiology, OSU.

# TEACHING EXPERIENCE

> 2001, 02 Lecturer, Tools for a Career in Cell and Molecular Biology,
> 1^st^ and 2^nd^ year graduate students, Oregon Health Sciences
> University
>
> 2000 Small Group Leader in Biological Basis of Disease, 1^st^ year
> medical students, Oregon Health Sciences University

2000. Lecturer, Fish Diseases Workshop. Hatfield Marine Sci. Center,
      Newport, OR

1988, 92 Laboratory Instructor, Fish Diseases Workshop. Hatfield Marine
Sci. Center, Newport, OR

1986. Graduate Teaching Assistant for General and Advanced Microbiology,
      Advances

> in Fish Diseases, and Microbial Ecology
>
> Post-doctoral fellows and students supervised:

#  Post Docs: Sima Hadidi (2006-08), Gavin Glenney (2004-05). NCCCWA

# Masters Student Rotation: Imke Veltman (1999) and Annmarie Lekkerkerker (1997), Agricultural University, Wageningen, Netherlands. OHSU

**GRANTS AND HONORS**

> 2007 National Research Initiative Competitive Grants Program, USDA,
> Flavobacterium 2007 Workshop \$10,000, Co-PI
>
> 2005 ARS/USDA Appropriated Funding: Host-Pathogen Interactions.
> 2005-2010, \$1,094,600/year, Lead-PI.
>
> 2003 NSF/USDA Microbial Genomics Grants Program, \$500,000, co-PI
>
> 2001 National Research Initiative Competitive Grants Program, USDA,
> \$147,817, PI
>
> 1999 American Cancer Society Research Grant, \$29,193, PI

1990 American Society for Microbiologists Travel Grant, \$600

1989 Sigma Xi Grant-in-Aid of Research, \$400

1988 Walter G. Jones Certificate of Excellence, Sea Grant, OSU

1986 Tartar Grant, \$2000

**PUBLICATIONS AND PATENTS** (\*= co-first authored papers)

1.  Turaga, P., **G.D. Wiens**, and S.L. Kaattari. 1987. Analysis of
    *Renibacterium salmoninarum* antigen production *in situ*. Fish
    Pathology. 22:209-214.

2.  Turaga, P., **G.D. Wiens**, and S.L. Kaattari. 1987. Bacterial
    Kidney Disease: The potential role of soluble protein antigen(s).
    Journal of Fish Biology, Supplement A 31:191-194.

3.  **Wiens, G.D.** and S.L. Kaattari. 1989. Monoclonal antibody
    analysis of common surface protein(s) of *Renibacterium
    salmoninarum*. Fish Pathology. 24:1-7.

4.  **Wiens, G.D.** and S.L. Kaattari. 1991. Monoclonal antibody
    characterization of a leukoagglutinin produced by *Renibacterium
    salmoninarum.* Infection and Immunity. 59:631-637.

5.  Rockey D.D., P.S.D. Turaga, **G.D. Wiens**, B.A Cook, and S.L.
    Kaattari. 1991. Serine proteinase of *Renibacterium salmoninarum*
    digests a major autologous extracellular and cell-surface protein.
    Canadian Journal of Microbiology. 37:758-763.

6.  Rockey D.D. L.L. Gilkey, **G.D. Wiens** and S.L. Kaattari. 1991.
    Monoclonal antibody-based analysis of the *Renibacterium
    salmoninarum* P57 protein in spawning Chinook and Coho salmon.
    Journal of Aquatic Animal Health. 3:23-30.

7.  Wood, P.A., **Wiens, G.D.**, Rohovec, J.S., Rockey, D.D. 1995.
    Identification of an immunologically cross-reactive 60-Kilodalton
    *Renibacterium salmoninarum* protein distinct from P57: Implications
    for immunodiagnostics. Journal of Aquatic Animal Health. 7:95-103.

8.  Martin, T.M., C. Kowalczyk, S. Stevens, **G.D. Wiens**, M.P.
    Stenzel-Poore, and M.B. Rittenberg. 1996. Deletion in HCDR3 rescues
    T15 antibody mutants from a secretion defect caused by mutations in
    HCDR2. The Journal of Immunology. 157:4341-4346.

9.  **Wiens, G.D.**, K.A. Heldwein, M.P. Stenzel-Poore, and M.B.
    Rittenberg. 1997. Somatic mutation in VH CDR2 and FRW2: Differential
    effects on antigen binding and immunoglobulin secretion. The Journal
    of Immunology. 159:1293-1302

10. Martin**\***, T.M, **G.D. Wiens\***, M.B. Rittenberg. 1998.
    Inefficient assembly and intracellular accumulation of antibodies
    with mutations in VH CDR2. The Journal of Immunology. 160:5963-5970.

11. Christensen, M., S. Kaattari, J. Piganelli, G. Wiens, and J.
    Zhang. 1998. *Renibacterium salmoninarum* vaccine and method for its
    preparation. United Kingdom Patent Number 2,308,300.

12. O\'Hare**\***, T., **G.D. Wiens\***, E.H. Whitcomb**\***, C.A. Enns
    and M.B. Rittenberg. 1999. Cutting Edge: Proteasome involvement in
    degradation of unassembled immunoglobulin light chains. The Journal
    of Immunology. 163:11-14.

13. Piganelli**\*** J.D., **G.D. Wiens\***, S.L. Kaattari. 1999.
    Elevated temperature treatment as a novel method for decreasing p57
    on the cell surface of *Renibacterium salmoninarum.* Diseases of
    Aquatic Organisms. 36:29-35.

14. Piganelli J.D., G.D. Wiens, J.A. Zhang, J.M. Christensen, S.L.
    Kaattari. 1999. Evaluation of a whole cell, p57^-^ vaccine against
    *Renibacterium salmoninarum.* Diseases of Aquatic Organisms.
    36:37-44.

15. Christensen, M., S. Kaattari, J. Piganelli, **G. Wiens**, and J.
    Zhang. 1999. *Renibacterium salmoninarum* vaccine and method for its
    preparation. U.S. Patent No. 5,871,751.

16. Brown, M., **G.D. Wiens**, T. O'Hare, M.P. Stenzel-Poore, M.B.
    Rittenberg. 1999. Replacements in the exposed loop of the T15
    antibody VH CDR2 affect carrier recognition of PC-containing
    pathogens. Molecular Immunology. 36:205-211.

17. **Wiens G.D.**, M.S. Chien, J.R. Winton, S.L. Kaattari. 1999.
    Antigenic and functional characterization of p57 produced by
    *Renibacterium salmoninarum.* Diseases of Aquatic Organisms.
    37:33-41.

18. Brown, M., M.A. Schumacher, **G.D. Wiens**, R.G. Brennan and M.B.
    Rittenberg. 2000. The structural basis of repertoire shift in an
    immune response to phosphocholine. Journal of Experimental Medicine.
    191:2101-2111.

19. **Wiens, G.D.**, A. Lekkerkerker, I. Veltman, and M.B.
    Rittenberg. 2001. Mutation of a single conserved residue in VH CDR2
    results in a severe Ig secretion defect. The Journal of Immunology.
    167:2179-2186.

20. **Wiens, G.D.**, T. O'Hare, and M.B. Rittenberg. 2001. Recovering
    antibody secretion using a hapten ligand as a chemical chaperone.
    Journal of Biological Chemistry. 276:40933-40939.

21. **Wiens, G.D.**, R. Pascho, and J.R. Winton. 2002. A single Ala^139^
    to Glu substitution in the *Renibacterium salmoninarum*
    virulence-associated protein p57 results in antigenic variation and
    is associated with its enhanced binding to Chinook salmon
    leukocytes. Applied and Environmental Microbiology. 68:3969-3977.

22. **Wiens, G.D.**, M. Brown, and M.B. Rittenberg. 2003. Repertoire
    shift in the humoral response to phosphocholine-keyhole limpet
    hemocyanin: V~H~ somatic mutation in germinal center B cells impairs
    T15 Ig function. The Journal of Immunology. 170:5095-5102.

23. Rodriquez, M.F., G.D. Wiens, M.K. Purcell, and Y. Palti. 2005
    Characterization of Toll-like receptor 3 gene in rainbow trout.
    Immunogenetics. 57(7):510-9

24. **Wiens, G.D.**, and J. Owen. 2005. Mapping neutralizing epitopes on
    *Renibacterium salmoninarum* p57 by use of transposon mutagenesis
    and synthetic peptides. Applied and Environmental Microbiology.
    71:2894-2901

25. Welch, T.J., and **G.D. Wiens.** 2005 Construction of a virulent,
    green fluorescent protein-tagged *Yersinia ruckeri* and detection in
    trout tissues after intraperitoneal and immersion challenge.
    Diseases of Aquatic Organisms. 67(3):267-72

26. **Wiens, G.D.**, Glenney, GW, LaPatra, SE, Welch, TJ. 2006
    Identification of novel rainbow trout (*Onchorynchus mykiss*)
    chemokines, CXCd1 and CXCd2: mRNA expression after *Yersinia
    ruckeri* vaccination and challenge. Immunogenetics. 58(4)308-23.

27. Glenney, G.W. and G.D. Wiens. 2007. Early diversification of the TNF
    superfamily in teleosts: Genomic characterization and expression
    analysis. The Journal of Immunology. 178:7955-7973.

28. Sudheesh, P.S., B.R. LaFrentz, D.R. Call, W.F. Siems, S.E. LaPatra,
    **G.D. Wiens**, and K.D. Cain. 2007. Identification of potential
    vaccine target antigens by immunoproteomic analysis of a
    non-virulent strain of the fish pathogen *Flavobacterium
    psychrophilum*. Diseases of Aquatic Organisms. 74(1):37-47.

29. Johnson, N.A., Vallejo, R.L., Silverstein, J.T., Welch, T.J.,
    **Wiens, G.D.**, Hallerman, E.M., and Palti, Y. 2008. Suggestive
    association of major histocompatibility IB genetic markers with
    resistance to bacterial cold water disease in rainbow trout
    (*Oncorhynchus mykiss*). Marine Biotechnology. 10:429-437.

30. Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., **Wiens,
    G.D.** 2008. Spleen size predicts resistance of rainbow trout to
    *Flavobacterium psychrophilum* challenge. The Journal of Immunology.
    180:4156-4165.

31. Shah D.H., K.D. Cain, **G.D. Wiens** and D.R. Call. 2008. Challenges
    associated with heterologous expression of *Flavobacterium
    psychrophilum* proteins in *Escherichia coli*. Marine Biotechnology.
    10:429-437

32. **Wiens, G.D.**, D.D. Rockey, Z. Wu, J. Chang, R. Levy, S. Crane,
    D.S. Chen, G.R. Capri, J.R. Burnett, P. Sudheesh, M.J. Schipma, H.
    Burd, A. Bhattacharyya, L.D. Rhodes, R. Kaul and M.S. Strom. 2008.
    The genome sequence of fish pathogen *Renibacterium salmoninarum*
    suggests reductive genome evolution away from an environmental
    *Arthrobacter* ancestor*.* Journal of Bacteriology.
    190(21):6970-6982.

33. Silverstein, J.T., Vallejo, R., Palti, Y., Leeds, T.D., Rexroad,
    C.E. III, Welch, T.J., **Wiens, G.D.** and Ducrocq, V. 2008. Rainbow
    trout innate resistance to bacterial cold-water disease is
    moderately heritable and is not adversely correlated with growth.
    Journal of Animal Science. In Press.

34. **Wiens, G.D.** and O.B. Dale. 2008. *Renibacterium salmoninarum*
    strains display geographic restriction in p57 antigenic variation
    and genomic markers. Diseases of Aquatic Organisms. In Press.

35. LaFrentz, B.R., S.E. LaPatra, D.R. Call, **G.D. Wiens** and K.D.
    Cain. Proteomic analysis of *Flavobacterium psychrophilum* cultured
    in vivo and in iron-limited media. In Preparation.

36. T. D. Leeds, J. T. Silverstein, R. L. Vallejo, Y. Palti, C. E.
    Rexroad III, T. J. Welch, and **G. D. Wiens.** Response to selection
    for bacterial cold water disease resistance in rainbow trout. In
    Preparation.

**Book Chapters and Reviews**

37. Wiens, G.D. 2009 Microbial Genomics of Aquaculture Pathogens
    (Chapter 12). In: K.E. Overturf ed. **Molecular Research in
    Aquaculture.** Submitted.

38. Wiens, G.D., and Welch, T. 2007. Recent advances in bacterial
    coldwater disease research. Trout Talk. Fall Issue.

39. Silverstein, J., Palti, Y., Vallejo, R.L., Welch, T.J., Wiens,
    G.D. 2006. Cracking the code: selecting for resistance against
    bacterial cold-water disease. Trout Talk.

40. Wiens, G.D. 2006. Bacterial Kidney Disease. (Updated from Wiens G,
    Kaattari SL, 1999. Bacterial Kidney Disease. In: Woo PTK, Bruno DW,
    eds. Fish Diseases and Disorders, Volume 3. Viral, Bacterial and
    Fungal Infections. Wallingford, UK: CAB International.) In:
    **Aquaculture Compendium.** Wallingford, UK: CAB International.

41. Wiens, GD. 2005. Fish Health Research at the National Center for
    Cool and Cold Water Aquaculture. Fish Tales Newsletter. West
    Virginia University Extension Service, Fall Newsletter (Reprinted in
    Trout Talk, the official newsletter of the US trout farmers
    association).

42. Wiens, G.D. and S.L. Kaattari. 1999 Bacterial Kidney Disease
    (*Renibacterium salmoninarum*). In: **Fish Disease and Disorders
    Volume 3: Viral, Bacterial and Fungal Infections**. P.T.K. Woo and
    D.W. Bruno, eds. Chapter 7. CABI Publishing, Wallington, Oxon, UK.

43. Wiens, G. D., V. A. Roberts, E.A. Whitcomb, T. O\'Hare, M.P.
    Stenzel-Poore, M.B. Rittenberg. 1998. Harmful somatic mutations:
    lessons from the dark side. Immunological Reviews. 162: 197-209

44. Wiens, G., P. Turaga, and S. Kaattari. 1990. Western Blot Analysis
    of a Fish Pathogen. In: **Techniques in Fish Immunology.** J.
    Stolen, T.C. Fletcher, D.P. Anderson, B.S. Roberson, and W.B. van
    Muiswinkel, eds. Chapter 10. SOS Publications, Fair Haven, N.J.

**Selected Meeting Abstracts**

1.  Wiens, G.D. 2008. Integrating Selective Breeding with Microbial
    Genomics to Improve Rainbow Trout Health. Eighth International
    Congress on Fish Biology, Portland, OR July 28^th^-31^st^

2.  Strom, M.S., D.R. Rockey, G.R. Capri, S. Ponnerassery, A.
    Bhattacharyya, R. Kaul, G.D. Wiens. 2007. Genome sequence of
    *Renibacterium salmoninarum* suggests divergence from environmental
    *Arthrobacter* spp. American Society for Microbiology General
    107^th^ Meeting, Toronto, Canada, May 21-25^th^.

3.  Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., Wiens,
    G.D. 2007. Spleen size predicts innate resistance of rainbow trout
    to *Flavobacterium psychrophilum* challenge. American Society for
    Microbiology General 107^th^ Meeting, Toronto, Canada, May
    21-25^th^.

4.  Wiens, G.D., Welch, T.J., Rexroad III, C.E., Lapatra, S., Call,
    D.R., Hunnicutt, D., Bhattacharyya, A., Campbell, J.,
    Walunas, T. 2007. Genome Sequence of *Flavobacterium psychrophilum*
    Strain CSF 259-93 and Characterization of a Large Cluster of Genes
    Containing Short Repeats. Flavobacterium 2007 Workshop, May2-4^th^
    Shepherdstown, WV Pg 12.