**OFFICE OF RESEARCH OVERSIGHT**

**GUIDANCE CHECKLIST FOR RESEARCH SAFETY**

**December 26, 2007**

**GENERAL INSTRUCTIONS:**

The following checklist has been prepared as a self-assessment tool for
the evaluation of VHA Research Safety Programs. The items in this
checklist can be attributed to various regulations and policies such as:

-   29 CFR 1910, Occupational Safety and Health Standards

-   29 CFR 1960, Basic Program Elements for Federal Employee
    Occupational Safety and Health Programs and Related Matters;

-   VA Directive 7700, Occupational Safety and Health;

-   VA Handbook 7700.1, Occupational Safety and Health Handbook;

-   VHA Directive 7701, Occupational Safety and Health Program;

-   VHA Handbook, 7701.1, Occupational Safety and Health Program
    Procedures;

-   VHA Handbook 1200.06, Control of Hazardous Agents in VA Research
    Laboratories;

-   VHA Handbook 1200.8, Safety of Personnel Engaged in Research;

-   Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th
    edition;

-   NIH Guidelines for Research Involving Recombinant DNA Molecules;

-   42 CFR 72 Possession, Use, and Transfer of Select Agents and Toxins;
    Final Rule (DHHS)

-   7 CFR 331 Agricultural Bioterrorism Protection Act of 2002 --
    Possession, Use, and Transfer of Biological Agents and Toxins; Final
    Rule (USDA: Plant and Plant Products);

-   9 CFR 121 Agricultural Bioterrorism Protection Act of 2002 --
    Possession, Use, and Transfer of Biological Agents and Toxins; Final
    Rule (USDA: Animal and Animal Products);

-   NFPA 101 and

-   Other applicable federal regulations, VHA Directives, VHA Handbooks,
    National Fire Protection Association (NFPA) standards, etc.

The checklist is comprehensive and intended to provide guidance for
developing and monitoring research safety program compliance.
**Facilities are encouraged to modify the checklist to address specific
requirements for their individual research programs** and are reminded
that they are responsible for ensuring the veracity and appropriateness
of all modifications.

-   Always refer to the precise text of cited source documents for more
    specific requirements or when the meaning of any checklist element
    is unclear.

-   The EPA regulations for hazardous waste must be considered in the
    context of individual state and local requirements, which may vary
    considerably.

-   Each biosafety level includes the requirements for that level in
    addition to those for lower levels.

**DOCUMENTS/SOURCE INFORMATION:**

The following documents will assist with the self-review and are
referenced by number in the "Documents/Source Information" column of the
checklist for your convenience.

1.  Copies of last 2 years Annual Workplace Evaluations (AWE) conducted
    by the OSH Network Team (VHA Handbook 7700.1.5.a; 7700.1.11.e) --
    only information relating to research areas is needed.

2.  Copies of last 2 years safety inspection reports and hazard
    surveillance surveys (of research areas) conducted by the Facility
    Occupational Safety and Health Program (VHA Handbook 7700.1.6.a;
    7700.1.11.f)

3.  Annual Vulnerability Assessment and any For-Cause Vulnerability
    Assessments of the Research Program conducted by Multi-Disciplinary
    Team

4.  Copy of any occupational injury and/or illness reports from the
    Research Laboratory to the Facility Safety and Occupational Health
    Management Office, if related to the Research Laboratories
    (information should be available through the ASISTS reporting
    system)

5.  Research Program Organizational Chart

6.  Research Safety Program and Research Service Safety Manual

7.  Medical Center Memoranda to establish various Subcommittees, if
    applicable

8.  R&D and SRS Committee Rosters with Membership Letters of Appointment
    (including terms)

9.  Minutes of the R&D, SRS, and other relevant Committees/Subcommittees
    for the last 12 months

10. SRS Policies, Procedures, and Plans

11. A list of all active protocols reviewed by the SRS for the last 12
    months

12. Safety Training Records for Investigators and all laboratory staff
    and any additional agent-specific training records, if applicable

13. Emergency Plan/Incident Response Plan, training records, and related
    drills

14. Research Chemical Hygiene Plan

15. Hazardous Agents Control Program

16. Copy of CDC/APHIS Certificate of Registration for Select Agents and
    Toxins, if applicable

17. Biosafety Manual(s), if applicable

18. BSL-3 Standard Operating Procedures/Manuals, if applicable

19. Infection Control Plan

20. Radiation Safety Program permits, if applicable

21. Radiation Safety Manual, if applicable

22. Copy of the inspection records conducted by external government
    agencies such as OIG, OSHA, CDC/APHIS and EPA, if applicable.

23. All Applicable MOUs and CRADO approvals

Appendix A explains acronyms used in this document.

+----------------------+---+---+---+---+---------+-----------+-----------+
| **A. RESEARCH SAFETY |   |   |   |   |         |           |           |
| PROGRAMS**           |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Determine that the |   |   |   |   |         |           |           |
| following items are  |   |   |   |   |         |           |           |
| appropriately and    |   |   |   |   |         |           |           |
| sufficiently         |   |   |   |   |         |           |           |
| implemented in your  |   |   |   |   |         |           |           |
| facility. Keep a     |   |   |   |   |         |           |           |
| completed version in |   |   |   |   |         |           |           |
| the Research Service |   |   |   |   |         |           |           |
| files.**             |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 1\. The VISN Safety  |   |   |   |   |         | VHA       | 1         |
| Office conducts an   |   |   |   |   |         | Directive |           |
| Annual Workplace     |   |   |   |   |         | 7701.3b;\ |           |
| Evaluation (AWE)     |   |   |   |   |         | VHA       |           |
| which includes:      |   |   |   |   |         | Handbook  |           |
|                      |   |   |   |   |         |           |           |
| > a\. a              |   |   |   |   |         | 7701      |           |
| > comprehensive      |   |   |   |   |         | .1.11.e;\ |           |
| > inspection using   |   |   |   |   |         | 77        |           |
| > the Safety         |   |   |   |   |         | 01.1.11.f |           |
| > Automated Facility |   |   |   |   |         |           |           |
| > Evaluation (SAFE)  |   |   |   |   |         |           |           |
| > software program;  |   |   |   |   |         |           |           |
| >                    |   |   |   |   |         |           |           |
| > b\. a program      |   |   |   |   |         |           |           |
| > review; and        |   |   |   |   |         |           |           |
| >                    |   |   |   |   |         |           |           |
| > c\. a written      |   |   |   |   |         |           |           |
| > report for the     |   |   |   |   |         |           |           |
| > research program   |   |   |   |   |         |           |           |
| > available for      |   |   |   |   |         |           |           |
| > review by the      |   |   |   |   |         |           |           |
| > facility.          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 2\. All research     |   |   |   |   |         | VHA       | 2         |
| labs are included in |   |   |   |   |         | Handbook  |           |
| the facility         |   |   |   |   |         | 12        |           |
| Occupational Safety  |   |   |   |   |         | 00.8.3.a; |           |
| and Health Program   |   |   |   |   |         |           |           |
| (OHSP) and they are  |   |   |   |   |         | 1200      |           |
| inspected at least   |   |   |   |   |         | .8.4.a.2; |           |
| annually by the      |   |   |   |   |         |           |           |
| environmental rounds |   |   |   |   |         | 77        |           |
| and research service |   |   |   |   |         | 01.1.11.e |           |
| personnel.           |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 3\. The Research     |   |   |   |   |         | VHA       | 5, 6, 7,  |
| Office maintains a   |   |   |   |   |         | Handbook  | 10        |
| Research Safety      |   |   |   |   |         |           |           |
| Program (RSP) that   |   |   |   |   |         | 1200.8.3; |           |
| encompasses          |   |   |   |   |         |           |           |
| biological,          |   |   |   |   |         | 1200.8.   |           |
| chemical, and        |   |   |   |   |         | 4.c.2.(d) |           |
| physical hazards in  |   |   |   |   |         |           |           |
| the research program |   |   |   |   |         |           |           |
| and is administered  |   |   |   |   |         |           |           |
| by the Research      |   |   |   |   |         |           |           |
| Safety Coordinator   |   |   |   |   |         |           |           |
| (RSC - frequently    |   |   |   |   |         |           |           |
| the ACOS/R&D) who is |   |   |   |   |         |           |           |
| appointed by the     |   |   |   |   |         |           |           |
| Research and         |   |   |   |   |         |           |           |
| Development          |   |   |   |   |         |           |           |
| Committee (R&D).     |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 4\. The Facility     |   |   |   |   | 29 CFR  | VHA       | 1         |
| Director authorizes  |   |   |   |   | 1960.9  | Handbook  |           |
| the RSC and          |   |   |   |   |         | 7701.     |           |
| appropriate research |   |   |   |   |         | 1.12.a.1; |           |
| service supervisors  |   |   |   |   |         |           |           |
| to establish and     |   |   |   |   |         | 120       |           |
| implement the OSHA   |   |   |   |   |         | 0.8.4.a.2 |           |
| program in           |   |   |   |   |         |           |           |
| accordance with the  |   |   |   |   |         |           |           |
| recommendations of   |   |   |   |   |         |           |           |
| the corresponding    |   |   |   |   |         |           |           |
| AWE.                 |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 5. Risks      |   |   |   |   | 29 CFR  |           | 1, 2, 4,  |
| to visitors and othe |   |   |   |   | 196     |           | 6, 10, 22 |
| r individuals with i |   |   |   |   | 0.59(a) |           |           |
| nfrequent access to  |   |   |   |   |         |           |           |
| research hazards and |   |   |   |   |         |           |           |
| /or areas (such as c |   |   |   |   |         |           |           |
| ontractors, engineer |   |   |   |   |         |           |           |
| ing, maintenance, an |   |   |   |   |         |           |           |
| d housekeeping perso |   |   |   |   |         |           |           |
| nnel) are assessed a |   |   |   |   |         |           |           |
| nd properly managed. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 6\. The RSC and      |   |   |   |   | 29 CFR  | VHA       | 1, 2, 6,  |
| research service     |   |   |   |   | 1960.9  | Handbook  | 9, 10     |
| supervisory          |   |   |   |   |         | 77        |           |
| personnel are        |   |   |   |   |         | 01.12.b.6 |           |
| notified of unsafe   |   |   |   |   |         |           |           |
| working conditions   |   |   |   |   |         |           |           |
| in research areas    |   |   |   |   |         |           |           |
| and they are         |   |   |   |   |         |           |           |
| responsible for      |   |   |   |   |         |           |           |
| ensuring appropriate |   |   |   |   |         |           |           |
| resolution or        |   |   |   |   |         |           |           |
| mitigation.          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 7\. There is a       |   |   |   |   | 10 CFR  | VHA       | 6, 9, 10, |
| Research Service     |   |   |   |   | 2       | Handbook  | 13, 20,   |
| safety manual        |   |   |   |   | 0.1101; |           | 21, 22    |
| (1200.8) and/or      |   |   |   |   |         | 1105.1    |           |
| Safety Plan          |   |   |   |   | 29 CFR  | 3.k(5);   |           |
| (1200.06) that       |   |   |   |   | 1910.   |           |           |
| includes biohazard   |   |   |   |   | 1450(c) | 1105.1    |           |
| controls and         |   |   |   |   |         | 3.m;      |           |
| emergency            |   |   |   |   |         |           |           |
| procedures.          |   |   |   |   |         | 1105.1    |           |
|                      |   |   |   |   |         | 4.a;      |           |
| a.  The Safety       |   |   |   |   |         |           |           |
|     Manual is        |   |   |   |   |         | 11        |           |
|     updated and      |   |   |   |   |         | 05.1.4.1; |           |
|     approved         |   |   |   |   |         |           |           |
|     annually by the  |   |   |   |   |         | 1200.     |           |
|     Subcommittee on  |   |   |   |   |         | 06.7.a.9; |           |
|     Research Safety  |   |   |   |   |         |           |           |
|     (SRS) and R&D.   |   |   |   |   |         | 1200.06.7 |           |
|                      |   |   |   |   |         | .h.4.(f); |           |
| b.  Individual labs  |   |   |   |   |         |           |           |
|     adhere to all    |   |   |   |   |         | 1200      |           |
|     applicable       |   |   |   |   |         | .8.3.a.1; |           |
|     federal safety   |   |   |   |   |         |           |           |
|     and health       |   |   |   |   |         | 1200      |           |
|     requirements.    |   |   |   |   |         | .8.4.c.1; |           |
|                      |   |   |   |   |         |           |           |
| c.  Non-exempt rDNA  |   |   |   |   |         | 12        |           |
|     research adheres |   |   |   |   |         | 00.8.4.c. |           |
|     to the NIH       |   |   |   |   |         | 2.(a--e); |           |
|     Guidelines and   |   |   |   |   |         |           |           |
|     is reviewed by a |   |   |   |   |         | Appendix  |           |
|     registered       |   |   |   |   |         | E         |           |
|     Institutional    |   |   |   |   |         |           |           |
|     Biosafety        |   |   |   |   |         | BMBL;     |           |
|     Committee (IBC   |   |   |   |   |         |           |           |
|     -- can be an     |   |   |   |   |         | NIH       |           |
|     augmented SRS).  |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
| d.  A Biosafety      |   |   |   |   |         |           |           |
|     Officer (BSO) is |   |   |   |   |         | Sec III,  |           |
|     appointed if     |   |   |   |   |         | Sec       |           |
|     non-exempt rDNA  |   |   |   |   |         | IV-B-1-c; |           |
|     research         |   |   |   |   |         |           |           |
|     involves Risk    |   |   |   |   |         | Sec       |           |
|     Group 3 agents   |   |   |   |   |         | IV-B-2-b  |           |
|     or large scale   |   |   |   |   |         |           |           |
|     production of    |   |   |   |   |         | Occ       |           |
|     viable rDNA      |   |   |   |   |         | upational |           |
|     molecules.       |   |   |   |   |         | Health    |           |
|                      |   |   |   |   |         | Guidebook |           |
| e.  The Facility has |   |   |   |   |         | for       |           |
|     a current permit |   |   |   |   |         | I         |           |
|     from the VA      |   |   |   |   |         | ndustrial |           |
|     Nuclear Health   |   |   |   |   |         | H         |           |
|     Physics Program  |   |   |   |   |         | ygienist, |           |
|     (NHPP) for       |   |   |   |   |         | Dept of   |           |
|     research         |   |   |   |   |         | Veterans  |           |
|     involving        |   |   |   |   |         | Affairs,  |           |
|     radioactive      |   |   |   |   |         | 1997, VA  |           |
|     compounds or     |   |   |   |   |         | Directive |           |
|     ionizing         |   |   |   |   |         | 5019      |           |
|     radiation.       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| f.  The Radiation    |   |   |   |   |         |           |           |
|     Safety Officer   |   |   |   |   |         |           |           |
|     (RSO) has        |   |   |   |   |         |           |           |
|     developed        |   |   |   |   |         |           |           |
|     written          |   |   |   |   |         |           |           |
|     procedures       |   |   |   |   |         |           |           |
|     (i.e., the       |   |   |   |   |         |           |           |
|     Radiation Safety |   |   |   |   |         |           |           |
|     Plan) for use of |   |   |   |   |         |           |           |
|     radioactive      |   |   |   |   |         |           |           |
|     materials in     |   |   |   |   |         |           |           |
|     research         |   |   |   |   |         |           |           |
|     laboratories.    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| g.  The RSO and      |   |   |   |   |         |           |           |
|     Radiation Safety |   |   |   |   |         |           |           |
|     Committee        |   |   |   |   |         |           |           |
|     (RadSC) annually |   |   |   |   |         |           |           |
|     review and       |   |   |   |   |         |           |           |
|     ensure the safe  |   |   |   |   |         |           |           |
|     and effective    |   |   |   |   |         |           |           |
|     use of ionizing  |   |   |   |   |         |           |           |
|     radiation and    |   |   |   |   |         |           |           |
|     radioactive      |   |   |   |   |         |           |           |
|     materials used   |   |   |   |   |         |           |           |
|     for research.    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| h.  The RSO and      |   |   |   |   |         |           |           |
|     RadSC have filed |   |   |   |   |         |           |           |
|     a written report |   |   |   |   |         |           |           |
|     with the NHPP    |   |   |   |   |         |           |           |
|     concerning any   |   |   |   |   |         |           |           |
|     incidents within |   |   |   |   |         |           |           |
|     the Research     |   |   |   |   |         |           |           |
|     Service.         |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| i.  An emergency     |   |   |   |   |         |           |           |
|     cascade phone    |   |   |   |   |         |           |           |
|     list is          |   |   |   |   |         |           |           |
|     included.        |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| j.  The Safety Plan  |   |   |   |   |         |           |           |
|     includes regular |   |   |   |   |         |           |           |
|     (at least        |   |   |   |   |         |           |           |
|     annual) safety   |   |   |   |   |         |           |           |
|     inspections of   |   |   |   |   |         |           |           |
|     the facility and |   |   |   |   |         |           |           |
|     drills or        |   |   |   |   |         |           |           |
|     exercises        |   |   |   |   |         |           |           |
|     conducted to     |   |   |   |   |         |           |           |
|     test and         |   |   |   |   |         |           |           |
|     evaluate the     |   |   |   |   |         |           |           |
|     effectiveness of |   |   |   |   |         |           |           |
|     the Plan.        |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 8\. High hazard      |   |   |   |   | []      | VA        | 1, 2, 6,  |
| areas of the medical |   |   |   |   | {.mark} | Handbook  | 10        |
| center, including    |   |   |   |   |         | 7700.1    |           |
| all research areas,  |   |   |   |   |         | 6.a       |           |
| are inspected        |   |   |   |   |         |           |           |
| quarterly by         |   |   |   |   |         |           |           |
| facility safety      |   |   |   |   |         |           |           |
| staff.               |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| > In addition, all   |   |   |   |   |         |           |           |
| > Research areas     |   |   |   |   |         |           |           |
| > should be          |   |   |   |   |         |           |           |
| > inspected annually |   |   |   |   |         |           |           |
| > by the Research    |   |   |   |   |         |           |           |
| > Safety Coordinator |   |   |   |   |         |           |           |
| > and VISN staff.    |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 9\. There is a       |   |   |   |   | 29 CFR  | VHA       | 6, 9, 10, |
| research chemical    |   |   |   |   | 1910.1  | Handbook\ | 11, 12,   |
| hygiene plan         |   |   |   |   | 450(e); | 1200.8.   | 14        |
| administered by the  |   |   |   |   |         | 3.a.2;    |           |
| Research Chemical    |   |   |   |   | 40 CFR  |           |           |
| Hygiene Officer      |   |   |   |   | 355     | 1200.8    |           |
| (CHO), who is        |   |   |   |   |         | 4.4.3;    |           |
| appointed by the     |   |   |   |   | 29 CFR  |           |           |
| SRS.                 |   |   |   |   | 19      | 1200.8.   |           |
|                      |   |   |   |   | 10.0148 | 4.d.4;    |           |
| a.  Material Safety  |   |   |   |   |         |           |           |
|     Data Sheets      |   |   |   |   | 29 CFR  | 1200.8.   |           |
|     (MSDS) are       |   |   |   |   | 19      | 4.d.14;   |           |
|     available for    |   |   |   |   | 10.1048 |           |           |
|     all hazardous    |   |   |   |   |         | 1200.8    |           |
|     chemicals.       |   |   |   |   | 29 CFR  | 4         |           |
|                      |   |   |   |   | 19      | .e.1.(c); |           |
| b.  SRS reviews and  |   |   |   |   | 10.1047 |           |           |
|     approves         |   |   |   |   |         | 1200.8    |           |
|     chemical hygiene |   |   |   |   |         | 4.        |           |
|     plan annually.   |   |   |   |   |         | e.14.(b); |           |
|                      |   |   |   |   |         |           |           |
| c.  Facility Safety  |   |   |   |   |         | 1200.06.  |           |
|                      |   |   |   |   |         | 7.g.1.(d) |           |
|   Officer/Industrial |   |   |   |   |         |           |           |
|     Hygienist        |   |   |   |   |         | 29 CFR    |           |
|     reviews and      |   |   |   |   |         | 1         |           |
|     approves a       |   |   |   |   |         | 910.1450, |           |
|     complete         |   |   |   |   |         |           |           |
|     inventory of     |   |   |   |   |         | Appendix  |           |
|     hazardous        |   |   |   |   |         | A         |           |
|     chemicals for    |   |   |   |   |         |           |           |
|     each protocol    |   |   |   |   |         |           |           |
|     prior to R&D     |   |   |   |   |         |           |           |
|     review.          |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| d.  Chemical         |   |   |   |   |         |           |           |
|     inventories are  |   |   |   |   |         |           |           |
|     continuously     |   |   |   |   |         |           |           |
|     updated,         |   |   |   |   |         |           |           |
|     reviewed, and    |   |   |   |   |         |           |           |
|     submitted to the |   |   |   |   |         |           |           |
|     CHO annually.    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| e                    |   |   |   |   |         |           |           |
| .  Chemical-specific |   |   |   |   |         |           |           |
|     training is      |   |   |   |   |         |           |           |
|     provided as      |   |   |   |   |         |           |           |
|     required by law  |   |   |   |   |         |           |           |
|     (formaldehyde,   |   |   |   |   |         |           |           |
|     ETO, etc.).      |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 10. All protocols    |   |   |   |   |         | VHA       | 6, 9, 10, |
|     using            |   |   |   |   |         | Handbook\ | 11        |
|     biological,      |   |   |   |   |         | 1200.8.   |           |
|     chemical,        |   |   |   |   |         | 4         |           |
|     physical, and/or |   |   |   |   |         | .c.2.(a); |           |
|     radiation        |   |   |   |   |         |           |           |
|     hazards are      |   |   |   |   |         | 1200.8.   |           |
|     reviewed and     |   |   |   |   |         | 4         |           |
|     approved by the  |   |   |   |   |         | .d.1.(b); |           |
|     SRS and R&D.     |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | 120       |           |
| > There is a         |   |   |   |   |         | 0.8.4.e.1 |           |
| > completed VA form  |   |   |   |   |         |           |           |
| > 10-0398 for        |   |   |   |   |         |           |           |
| > VA-funded          |   |   |   |   |         |           |           |
| > protocols (or      |   |   |   |   |         |           |           |
| > equivalent for non |   |   |   |   |         |           |           |
| > VA-funded          |   |   |   |   |         |           |           |
| > protocols) on      |   |   |   |   |         |           |           |
| > file.              |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 11\. The Facility    |   |   |   |   | 29 CFR  | []{.mark} | 1, 2, 3,  |
| Director (or         |   |   |   |   | 196     |           | 6, 10,    |
| designee)            |   |   |   |   | 0.59(a) |           | 13, 14,   |
| establishes          |   |   |   |   |         |           | 16, 17,   |
| clearance procedures |   |   |   |   |         |           | 18, 21,   |
| for visitors and     |   |   |   |   |         |           | 22        |
| other individuals    |   |   |   |   |         |           |           |
| with an infrequent   |   |   |   |   |         |           |           |
| need to access       |   |   |   |   |         |           |           |
| research laboratory  |   |   |   |   |         |           |           |
| areas (chemical and  |   |   |   |   |         |           |           |
| biological agent     |   |   |   |   |         |           |           |
| disposal, laboratory |   |   |   |   |         |           |           |
| cleaning, pest       |   |   |   |   |         |           |           |
| management services, |   |   |   |   |         |           |           |
| security, etc.).     |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 12\. A               |   |   |   |   |         | VHA       | 3, 6, 10  |
| multidisciplinary    |   |   |   |   |         | Handbook  |           |
| team of local        |   |   |   |   |         | 1200.06.  |           |
| research personnel,  |   |   |   |   |         | 7.h.4.(j) |           |
| a VA Police Service  |   |   |   |   |         |           |           |
| representative, the  |   |   |   |   |         | 7700.1;   |           |
| Facility Safety      |   |   |   |   |         |           |           |
| Officer, Safety      |   |   |   |   |         | 0730/1,   |           |
| Manager, RSC,        |   |   |   |   |         | paragraph |           |
| Research CHO,        |   |   |   |   |         | 6.        |           |
| Emergency Manager,   |   |   |   |   |         |           |           |
| RSO, and/or          |   |   |   |   |         |           |           |
| Industrial Hygienist |   |   |   |   |         |           |           |
| conduct initial and  |   |   |   |   |         |           |           |
| annual vulnerability |   |   |   |   |         |           |           |
| assessments of all   |   |   |   |   |         |           |           |
| labs.                |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 13\. The same team   |   |   |   |   |         | VHA       | 3, 6, 10  |
| conducts             |   |   |   |   |         | Handbook  |           |
| vulnerability        |   |   |   |   |         | 1200.06.  |           |
| assessments after    |   |   |   |   |         | 7.h.4.(j) |           |
| any incident         |   |   |   |   |         |           |           |
| affecting high risk  |   |   |   |   |         | 7700.1;   |           |
| areas, materials, or |   |   |   |   |         |           |           |
| physical security of |   |   |   |   |         | 0730/1,   |           |
| research             |   |   |   |   |         | paragraph |           |
| laboratories and     |   |   |   |   |         | 6.        |           |
| reported to the SRS, |   |   |   |   |         |           |           |
| R&D, and Responsible |   |   |   |   |         |           |           |
| Official, if         |   |   |   |   |         |           |           |
| applicable.          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 14\. There is a      |   |   |   |   |         | VHA       | 3, 6, 9,  |
| Facility Emergency   |   |   |   |   |         | Handbook  | 10, 13    |
| Preparedness         |   |   |   |   |         |           |           |
| Response Plan (EPRP) |   |   |   |   |         | 1200      |           |
| that includes the    |   |   |   |   |         | .06.7.a.9 |           |
| research program and |   |   |   |   |         |           |           |
| which is:            |   |   |   |   |         | 12        |           |
|                      |   |   |   |   |         | 00.06.7.h |           |
| > a\. Reviewed       |   |   |   |   |         |           |           |
| > annually and       |   |   |   |   |         | 1200.06.  |           |
| >                    |   |   |   |   |         | 7.h.4.(i) |           |
| > b\. Practiced      |   |   |   |   |         |           |           |
| > annually.          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 15\. Biological      |   |   |   |   | 29 CFR  | VHA       | 6, 10, 19 |
| fluids are handled   |   |   |   |   | 1910.   | Handbook\ |           |
| using universal      |   |   |   |   | 1030(d) | 1200.8.   |           |
| precautions          |   |   |   |   |         | 3.a.1.(a) |           |
| according to         |   |   |   |   |         |           |           |
| bloodborne pathogen  |   |   |   |   |         |           |           |
| standards and in     |   |   |   |   |         |           |           |
| accordance with the  |   |   |   |   |         |           |           |
| Research facility's  |   |   |   |   |         |           |           |
| Bloodborne Pathogen  |   |   |   |   |         |           |           |
| Exposure Control     |   |   |   |   |         |           |           |
| Plan.                |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 16\. All fume hoods, |   |   |   |   | 29 CFR  | VHA       | 1, 2, 6,  |
| laminar flow         |   |   |   |   | 1910.1  | Handbook\ | 10,       |
| chemical hoods, and  |   |   |   |   | 450(e)( | 120       |           |
| biological safety    |   |   |   |   | 3)(iii) | 0.8.4.e.5 |           |
| cabinets (BSCs) are  |   |   |   |   |         |           |           |
| certified annually   |   |   |   |   |         | 29 CFR    |           |
| (semi-annually for   |   |   |   |   |         | 1910.1450 |           |
| airborne             |   |   |   |   |         | Appendix  |           |
| pathogens).\         |   |   |   |   |         | A         |           |
| *Note: In addition   |   |   |   |   |         |           |           |
| to                   |   |   |   |   |         |           |           |
| annual/semi-annual   |   |   |   |   |         |           |           |
| certification,       |   |   |   |   |         |           |           |
| portable BSCs must   |   |   |   |   |         |           |           |
| be recertified       |   |   |   |   |         |           |           |
| whenever they are    |   |   |   |   |         |           |           |
| moved.*              |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| **B. SUBCOMMITTEE ON |   |   |   |   |         |           |           |
| RESEARCH SAFETY      |   |   |   |   |         |           |           |
| (SRS)**              |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 1\. Committee        |   |   |   |   |         | VHA       | 6, 7, 8,  |
| Composition complies |   |   |   |   |         | Handbook\ | 9, 10     |
| with VHA             |   |   |   |   |         | 12        |           |
| requirements.        |   |   |   |   |         | 00.8.4.d; |           |
|                      |   |   |   |   |         |           |           |
| a.  At least 5       |   |   |   |   |         | VHA       |           |
|     voting members   |   |   |   |   |         | Handbook\ |           |
|     nominated by the |   |   |   |   |         | 1200.8.   |           |
|     SRS and R&D      |   |   |   |   |         | App. C    |           |
|     Committee and    |   |   |   |   |         |           |           |
|     appointed for a  |   |   |   |   |         |           |           |
|     specified term   |   |   |   |   |         |           |           |
|     by the Medical   |   |   |   |   |         |           |           |
|     Center           |   |   |   |   |         |           |           |
|     Director (MCD)   |   |   |   |   |         |           |           |
|     including:       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Chair --     |   |   |   |   |         |           |           |
|         appointed by |   |   |   |   |         |           |           |
|         MCD for      |   |   |   |   |         |           |           |
|         1-year       |   |   |   |   |         |           |           |
|         renewable    |   |   |   |   |         |           |           |
|         term         |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Members with |   |   |   |   |         |           |           |
|         appropriate  |   |   |   |   |         |           |           |
|         expertise in |   |   |   |   |         |           |           |
|         etiological  |   |   |   |   |         |           |           |
|         agents,      |   |   |   |   |         |           |           |
|         chemical     |   |   |   |   |         |           |           |
|         hazards,     |   |   |   |   |         |           |           |
|         and/or       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|   physical/radiation |   |   |   |   |         |           |           |
|         hazards, as  |   |   |   |   |         |           |           |
|         applicable   |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Minimum of 2 |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|       non-affiliated |   |   |   |   |         |           |           |
|         members when |   |   |   |   |         |           |           |
|         non-exempt   |   |   |   |   |         |           |           |
|         rDNA         |   |   |   |   |         |           |           |
|         research is  |   |   |   |   |         |           |           |
|         conducted    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Biological   |   |   |   |   |         |           |           |
|         Safety       |   |   |   |   |         |           |           |
|         Officer      |   |   |   |   |         |           |           |
|         (BSO), as    |   |   |   |   |         |           |           |
|         applicable   |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Facility     |   |   |   |   |         |           |           |
|         Safety       |   |   |   |   |         |           |           |
|         Officer and  |   |   |   |   |         |           |           |
|         IH,          |   |   |   |   |         |           |           |
|         recommended  |   |   |   |   |         |           |           |
|         as voting    |   |   |   |   |         |           |           |
|         member(s)    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| ```{=html}           |   |   |   |   |         |           |           |
| <!-- -->             |   |   |   |   |         |           |           |
| ```                  |   |   |   |   |         |           |           |
| b.  Ex-Officio       |   |   |   |   |         |           |           |
|     members          |   |   |   |   |         |           |           |
|     including:       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   R&D          |   |   |   |   |         |           |           |
|         Committee    |   |   |   |   |         |           |           |
|         Liason       |   |   |   |   |         |           |           |
|         (voting)     |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     -   Research CHO |   |   |   |   |         |           |           |
|         (voting), as |   |   |   |   |         |           |           |
|         applicable   |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| -   AO/R&D or R&D    |   |   |   |   |         |           |           |
|     Office           |   |   |   |   |         |           |           |
|     Representative   |   |   |   |   |         |           |           |
|     (non-voting)     |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| -   Employee Union   |   |   |   |   |         |           |           |
|     Representative,  |   |   |   |   |         |           |           |
|     as determined by |   |   |   |   |         |           |           |
|     the local union  |   |   |   |   |         |           |           |
|     contract.        |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 2\. Meets quarterly  |   |   |   |   |         | VHA       | 9, 10     |
| at minimum           |   |   |   |   |         | Handbook\ |           |
|                      |   |   |   |   |         | 1200      |           |
|                      |   |   |   |   |         | .8.4.d.11 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 3\. Reviews and      |   |   |   |   |         | VHA       | 9, 10, 11 |
| approves/disapproves |   |   |   |   |         | Handbook\ |           |
| all new research     |   |   |   |   |         | 1200.8.   |           |
| involving            |   |   |   |   |         | 4.d.1.(b) |           |
| biological,          |   |   |   |   |         |           |           |
| chemical, physical,  |   |   |   |   |         |           |           |
| and radiation        |   |   |   |   |         |           |           |
| hazards at convened  |   |   |   |   |         |           |           |
| meetings with a      |   |   |   |   |         |           |           |
| quorum present       |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 4\. Annually reviews |   |   |   |   |         | VHA       | 9, 10, 11 |
| all active research  |   |   |   |   |         | Handbook\ |           |
| protocols involving  |   |   |   |   |         | 120       |           |
| safety hazards       |   |   |   |   |         | 0.8.4.d.3 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 5\. Annually         |   |   |   |   | 29 CFR  | VHA       | 9, 10,    |
| coordinates/provides |   |   |   |   | 1910.   | Handbook\ | 11, 12    |
| research specific    |   |   |   |   | 1450(f) | 1200      |           |
| safety training for  |   |   |   |   |         | .8.4.d.5; |           |
| all laboratory       |   |   |   |   |         |           |           |
| personnel.           |   |   |   |   |         | 1200      |           |
|                      |   |   |   |   |         | .8.4.d.10 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 6\. Ensures all      |   |   |   |   |         | VHA       | 1, 2, 9,  |
| deficiencies cited   |   |   |   |   |         | Handbook  | 10        |
| during safety        |   |   |   |   |         | 1200.8.   |           |
| inspections are      |   |   |   |   |         | 4.d.5.(a) |           |
| properly abated.     |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 7\. Reports          |   |   |   |   |         | VHA       | 1, 2, 9,  |
| operational problems |   |   |   |   |         | Handbook\ | 10        |
| and/or violations to |   |   |   |   |         | 120       |           |
| RSC within 30 days,  |   |   |   |   |         | 0.8.4.d.6 |           |
| unless PI has done   |   |   |   |   |         |           |           |
| so.                  |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 8.  Ensures proper   |   |   |   |   | 29 CFR  | VHA       | 1, 2, 4,  |
|     health           |   |   |   |   | 1910.   | Handbook\ | 6, 9, 10, |
|     surveillance and |   |   |   |   | 1450(c) | 1200      | 11, 12,   |
|     exposure         |   |   |   |   |         | .8.4.d.7; | 19        |
|     monitoring of    |   |   |   |   |         |           |           |
|     personnel.       |   |   |   |   |         | 1200      |           |
|                      |   |   |   |   |         | .8.4.d.13 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 9\. Records and      |   |   |   |   |         | VHA       | 6, 9, 10  |
| forwards minutes to  |   |   |   |   |         | Handbook\ |           |
| the R&D for review   |   |   |   |   |         | 1200.8    |           |
| and approval.        |   |   |   |   |         | .4.d.8-9; |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | 1200.8.   |           |
|                      |   |   |   |   |         | App. D    |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 10\. Use of an       |   |   |   |   |         | VHA       | 5, 6, 8,  |
| affiliate Safety     |   |   |   |   |         | Handbook  | 23        |
| Committee must be    |   |   |   |   |         | 1200.8.4. |           |
| pre-approved by      |   |   |   |   |         | c.1.(a-b) |           |
| CRADO and include at |   |   |   |   |         |           |           |
| least one qualified  |   |   |   |   |         |           |           |
| VA employee.         |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| **C. LABORATORY      |   |   |   |   |         |           |           |
| SAFETY - GENERAL**   |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 1\. The work area is |   |   |   |   | 29 CFR  | 29 CFR    | 1, 2, 6,  |
| free of unnecessary  |   |   |   |   | 1910.1  | 1910.1450 | 10, 22    |
| clutter. Fume hoods  |   |   |   |   | 450(e)( | Appendix  |           |
| and BSC are not used |   |   |   |   | 3)(iii) | A         |           |
| for general storage  |   |   |   |   |         |           |           |
| and have             |   |   |   |   |         |           |           |
| vents/ductwork       |   |   |   |   |         |           |           |
| unobstructed.        |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 2. Carpet     |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| s and/or rugs are no |   |   |   |   | 1910.   |           | 10, 14,   |
| t used in labs or wo |   |   |   |   | 1030(e) |           | 15, 17,   |
| rk areas where biolo |   |   |   |   | (3)(ii) |           | 18, 22    |
| gical or chemical ma |   |   |   |   |         |           |           |
| terials are handled. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 3\. For rooms        |   |   |   |   |         | National  | 1, 2, 6,  |
| equipped with        |   |   |   |   |         | Fire      | 10, 22    |
| sprinklers, all      |   |   |   |   |         | P         |           |
| items on shelves     |   |   |   |   |         | rotection |           |
| have a minimum       |   |   |   |   |         | As        |           |
| vertical clearance   |   |   |   |   |         | sociation |           |
| of 18-inches from    |   |   |   |   |         | (NFPA)    |           |
| sprinkler heads,     |   |   |   |   |         | Standard  |           |
| heating pipes, and   |   |   |   |   |         | 13        |           |
| lighting fixtures.   |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | (NFPA     |           |
|                      |   |   |   |   |         | 13-5.5.6) |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 4\. Functional fire  |   |   |   |   | 29 CFR  | NFPA 10   | 1, 2, 6,  |
| extinguishers,       |   |   |   |   | 1910    | (Standard | 10, 22    |
| appropriate for the  |   |   |   |   | .157(c) | for       |           |
| hazard(s) present,   |   |   |   |   | & (d)   | Portable  |           |
| are readily          |   |   |   |   |         | Fire      |           |
| available and        |   |   |   |   |         | Extin     |           |
| unobstructed.        |   |   |   |   |         | guishers) |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NFPA      |           |
|                      |   |   |   |   |         | 10        |           |
|                      |   |   |   |   |         | 1-7.7.4.1 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 5\. Personnel have   |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| access to emergency  |   |   |   |   | 1910    |           | 10, 22    |
| first aid services   |   |   |   |   | .151(a) |           |           |
| or kits.             |   |   |   |   | & (b)   |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 6\. Safety showers   |   |   |   |   | 29 CFR  | BMBL      | 1, 2, 6,  |
| and eyewashes are    |   |   |   |   | 1       | BSL2.D.8; | 10, 14,   |
| located wherever     |   |   |   |   | 910.151 |           | 17, 18,   |
| hazardous agents are |   |   |   |   | (c)     | A         | 22        |
| used.                |   |   |   |   |         | BSL2.D.14 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 7\. Eyewash and      |   |   |   |   | 29 CFR  | BMBL      | 1, 2, 6,  |
| safety showers are   |   |   |   |   | 1       | BSL2.D.8; | 10, 14,   |
| tested and results   |   |   |   |   | 910.151 |           | 17, 18,   |
| are documented in    |   |   |   |   | (c)     | A         | 22        |
| accordance with      |   |   |   |   |         | BSL2.D.14 |           |
| facility policy.     |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 8\. An occupational  |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| noise monitoring     |   |   |   |   | 1910.95 |           | 10, 22    |
| program is           |   |   |   |   |         |           |           |
| implemented when     |   |   |   |   |         |           |           |
| potentially          |   |   |   |   |         |           |           |
| hazardous noise      |   |   |   |   |         |           |           |
| exists.              |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 9\. Personnel who    |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| are required to use  |   |   |   |   | 1910.   |           | 10, 12,   |
| respirators have     |   |   |   |   | 134(c), |           | 22        |
| undergone medical    |   |   |   |   | (e),    |           |           |
| evaluations and have |   |   |   |   | (f), &  |           |           |
| been properly        |   |   |   |   | (k)     |           |           |
| trained and fitted   |   |   |   |   |         |           |           |
| for their use.       |   |   |   |   | 29 CFR  |           |           |
| Personnel who are    |   |   |   |   | 1910.   |           |           |
| permitted to         |   |   |   |   | 1450(i) |           |           |
| voluntarily use      |   |   |   |   |         |           |           |
| respirators are      |   |   |   |   |         |           |           |
| provided a copy of   |   |   |   |   |         |           |           |
| 29 CFR 1910.134      |   |   |   |   |         |           |           |
| Appendix D           |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 10\. Use of          |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| extension cords is   |   |   |   |   | 1       |           | 10, 22    |
| approved by RSC and  |   |   |   |   | 910.305 |           |           |
| minimized.           |   |   |   |   | (g)(1)( |           |           |
|                      |   |   |   |   | ii)(A); |           |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   | 29 CFR  |           |           |
|                      |   |   |   |   | 1910.30 |           |           |
|                      |   |   |   |   | 5(a)(2) |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 11\. Ground fault    |   |   |   |   |         | NEC       | 1, 2, 6,  |
| interrupter (GFI)    |   |   |   |   |         | (Article  | 10, 22    |
| electrical outlets   |   |   |   |   |         | 210-8);   |           |
| are used in wet or   |   |   |   |   |         |           |           |
| high risk areas.     |   |   |   |   |         | NFPA 79;  |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NFPA 70E  |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 12\. Compressed gas  |   |   |   |   | 29 CFR  | American  | 1, 2, 6,  |
| cylinders are        |   |   |   |   | 19      | Gas       | 10, 22    |
| transported on       |   |   |   |   | 10.101; | As        |           |
| cylinder carts and   |   |   |   |   |         | sociation |           |
| secured with chains  |   |   |   |   |         | (AGA)     |           |
| or straps and capped |   |   |   |   |         | Pamphlet  |           |
| when not in use.     |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 13. Hazardous        |   |   |   |   | 29 CFR  | NFPA 45   | 1, 2, 6,  |
|     chemical storage |   |   |   |   | 1910.   |           | 10, 14,   |
|     is:              |   |   |   |   | 106(d)( | 29 CFR    | 15, 22    |
|                      |   |   |   |   | 1)-(4); | 1910.1450 |           |
|     a.  In properly  |   |   |   |   |         | Appendix  |           |
|         identified   |   |   |   |   |         | A         |           |
|         and          |   |   |   |   |         |           |           |
|         compatible   |   |   |   |   |         |           |           |
|         containers   |   |   |   |   |         |           |           |
|         and in a     |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|        non-flammable |   |   |   |   |         |           |           |
|         storage room |   |   |   |   |         |           |           |
|         OR           |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|        non-flammable |   |   |   |   |         |           |           |
|         cabinets;    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     b.  Below eye    |   |   |   |   |         |           |           |
|         level, but   |   |   |   |   |         |           |           |
|         not on the   |   |   |   |   |         |           |           |
|         floor;       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     c.  Segregated   |   |   |   |   |         |           |           |
|         according to |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|        compatibility |   |   |   |   |         |           |           |
|         (flammables  |   |   |   |   |         |           |           |
|         separate     |   |   |   |   |         |           |           |
|         from         |   |   |   |   |         |           |           |
|         oxidizers,   |   |   |   |   |         |           |           |
|         acids        |   |   |   |   |         |           |           |
|         separate     |   |   |   |   |         |           |           |
|         from bases,  |   |   |   |   |         |           |           |
|         etc.);       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     d.  Appropriate  |   |   |   |   |         |           |           |
|         for          |   |   |   |   |         |           |           |
|         flammable    |   |   |   |   |         |           |           |
|         liquids      |   |   |   |   |         |           |           |
|         (safety cans |   |   |   |   |         |           |           |
|         for volumes  |   |   |   |   |         |           |           |
|         greater than |   |   |   |   |         |           |           |
|         4-L and      |   |   |   |   |         |           |           |
|         cabinets for |   |   |   |   |         |           |           |
|         volumes      |   |   |   |   |         |           |           |
|         greater than |   |   |   |   |         |           |           |
|         10-L);       |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     e.  Within       |   |   |   |   |         |           |           |
|         appropriate  |   |   |   |   |         |           |           |
|         cabinetry if |   |   |   |   |         |           |           |
|         flammable or |   |   |   |   |         |           |           |
|         corrosive;   |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     f.  In           |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|      explosion-proof |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|        refrigerators |   |   |   |   |         |           |           |
|         when         |   |   |   |   |         |           |           |
|         flammable    |   |   |   |   |         |           |           |
|         cold storage |   |   |   |   |         |           |           |
|         is required; |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     g.  Monitored    |   |   |   |   |         |           |           |
|         for disposal |   |   |   |   |         |           |           |
|         prior to     |   |   |   |   |         |           |           |
|         expiration   |   |   |   |   |         |           |           |
|         --           |   |   |   |   |         |           |           |
|         particularly |   |   |   |   |         |           |           |
|         for          |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     peroxide-forming |   |   |   |   |         |           |           |
|         reagents;    |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|     h.  Examined     |   |   |   |   |         |           |           |
|         periodically |   |   |   |   |         |           |           |
|         (at least    |   |   |   |   |         |           |           |
|         annually)    |   |   |   |   |         |           |           |
|         for          |   |   |   |   |         |           |           |
|         replacement, |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
|       deterioration, |   |   |   |   |         |           |           |
|         and          |   |   |   |   |         |           |           |
|         container    |   |   |   |   |         |           |           |
|         integrity.   |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 14\. All chemical    |   |   |   |   | 40 CFR  | 29 CFR    | 1, 2, 6,  |
| and                  |   |   |   |   | 262     | 1910.1450 | 10, 14,   |
| biological/medical   |   |   |   |   | .43(c), | Appendix  | 15, 22    |
| lab wastes are       |   |   |   |   | subpart | A         |           |
| collected and stored |   |   |   |   | C (EPA) |           |           |
| in compatible        |   |   |   |   |         |           |           |
| containers. Waste    |   |   |   |   | 29 CFR  |           |           |
| accumulation is      |   |   |   |   | 19      |           |           |
| minimized and        |   |   |   |   | 10.1030 |           |           |
| limited to the lab   |   |   |   |   | (c)(    |           |           |
| area. Waste disposal |   |   |   |   | 4)(iii) |           |           |
| is in accordance     |   |   |   |   | (re     |           |           |
| with federal and     |   |   |   |   | gulated |           |           |
| [state]{.underline}  |   |   |   |   | biol    |           |           |
| regulations.         |   |   |   |   | ogical/ |           |           |
|                      |   |   |   |   | medical |           |           |
|                      |   |   |   |   | wastes) |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 15\. Spill control   |   |   |   |   |         | VHA       | 1, 2, 6,  |
| programs are in      |   |   |   |   |         | Handbook  | 10, 12,   |
| place and may        |   |   |   |   |         | 1200.0    | 13, 14,   |
| include spill kits.  |   |   |   |   |         | 6.7.h.4.b | 15, 19,   |
|                      |   |   |   |   |         |           | 22        |
| > Training is        |   |   |   |   |         | NIH       |           |
| > provided when      |   |   |   |   |         | G         |           |
| > spill kits are     |   |   |   |   |         | uidelines |           |
| > available.         |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec       |           |
|                      |   |   |   |   |         | IV-       |           |
|                      |   |   |   |   |         | B-2-6-(6) |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec       |           |
|                      |   |   |   |   |         | IV-       |           |
|                      |   |   |   |   |         | B-3-c-(3) |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | BMBL      |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | B         |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 16\. Controlled      |   |   |   |   | 21 CFR  | VHA       | 1, 2, 6,  |
| substances are       |   |   |   |   | 130     | Handbook  | 10, 22    |
| properly secured and |   |   |   |   | 1.72-76 |           |           |
| inventoried.         |   |   |   |   |         | 1108.1.14 |           |
|                      |   |   |   |   | 21 CFR  |           |           |
|                      |   |   |   |   | 1304.11 |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 17\. Select          |   |   |   |   | 29 CFR  |           | 1, 2, 6,  |
| carcinogens,         |   |   |   |   | 1910.14 |           | 10, 12,   |
| reproductive toxins, |   |   |   |   | 50(e)(3 |           | 14, 15,   |
| and substances with  |   |   |   |   | )(viii) |           | 16, 17,   |
| a high degree of     |   |   |   |   |         |           | 18, 22    |
| acute toxicity are   |   |   |   |   |         |           |           |
| handled in           |   |   |   |   |         |           |           |
| designated areas.    |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 18\. Eating,         |   |   |   |   | 29 CFR  | 29 CFR    | 1, 2, 6,  |
| drinking, smoking,   |   |   |   |   | 1910.   | 1910.1450 | 10, 14,   |
| handling contact     |   |   |   |   | 1450(e) | Appendix  | 15, 22    |
| lenses, and/or       |   |   |   |   | (3)(ii) | A E.1.(d) |           |
| applying cosmetics   |   |   |   |   |         |           |           |
| are prohibited in    |   |   |   |   |         |           |           |
| all labs and/or      |   |   |   |   |         |           |           |
| areas where          |   |   |   |   |         |           |           |
| chemicals are used.  |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 19\. A personal      |   |   |   |   | 29 CFR  |           | 6, 10,    |
| protective equipment |   |   |   |   | [19     |           | 11, 12,   |
| (PPE) hazard         |   |   |   |   | 10.132( |           | 14, 15,   |
| assessment must be   |   |   |   |   | d)](htt |           | 17, 18,   |
| conducted for each   |   |   |   |   | p://www |           | 19        |
| lab. A copy of the   |   |   |   |   | .osha.g |           |           |
| certified hazard     |   |   |   |   | ov/pls/ |           |           |
| assessment must be   |   |   |   |   | oshaweb |           |           |
| maintained in each   |   |   |   |   | /owalin |           |           |
| laboratory. All      |   |   |   |   | k.query |           |           |
| employees working in |   |   |   |   | _links? |           |           |
| the lab must be      |   |   |   |   | src_doc |           |           |
| trained in the PPE   |   |   |   |   | _type=S |           |           |
| required for use in  |   |   |   |   | TANDARD |           |           |
| the lab.             |   |   |   |   | S&src_u |           |           |
|                      |   |   |   |   | nique_f |           |           |
|                      |   |   |   |   | ile=191 |           |           |
|                      |   |   |   |   | 0_0132& |           |           |
|                      |   |   |   |   | src_anc |           |           |
|                      |   |   |   |   | hor_nam |           |           |
|                      |   |   |   |   | e=1910. |           |           |
|                      |   |   |   |   | 132(d)) |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| 20\. PPE must be     |   |   |   |   | 29 CFR  |           | 6, 10,    |
| provided, used, and  |   |   |   |   | 1910    |           | 11, 12,   |
| maintained in        |   |   |   |   | .132(a) |           | 14, 15,   |
| accordance with OSHA |   |   |   |   |         |           | 17, 18,   |
| requirements and     |   |   |   |   |         |           | 19        |
| facility policies,   |   |   |   |   |         |           |           |
| (i.e., removal prior |   |   |   |   |         |           |           |
| to leaving work      |   |   |   |   |         |           |           |
| area, cleaning and   |   |   |   |   |         |           |           |
| laundering,          |   |   |   |   |         |           |           |
| training,            |   |   |   |   |         |           |           |
| maintenance & care). |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| **D. BIOSAFETY (BSL) |   |   |   |   |         |           |           |
| 1 AND ANIMAL         |   |   |   |   |         |           |           |
| BIOSAFETY LEVEL      |   |   |   |   |         |           |           |
| (ABSL) 1             |   |   |   |   |         |           |           |
| LABORATORIES**       |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Standard           |   |   |   |   |         |           |           |
| Microbiological      |   |   |   |   |         |           |           |
| Practices**          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 1. The La     |   |   |   |   |         | VHA       | 3, 6, 10, |
| b or Animal Facility |   |   |   |   |         | Handbook  | 17        |
|  Director (PI if non |   |   |   |   |         |           |           |
| exempt rDNA) control |   |   |   |   |         | 1200      |           |
| s access to labs or  |   |   |   |   |         | .06.4.k.2 |           |
| facilities in accord |   |   |   |   |         |           |           |
| ance with local inst |   |   |   |   |         | BMBL Sec  |           |
| itutional policies.  |   |   |   |   |         | IV BSL-1  |           |
|                      |   |   |   |   |         | A.1;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec IV    |           |
|                      |   |   |   |   |         | BSL-1     |           |
|                      |   |   |   |   |         | A.11;     |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | A.6;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-1-a |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 2.            |   |   |   |   |         | BMBL Sec  | 6, 10,    |
| Personnel are traine |   |   |   |   |         | IV BSL-1  | 12, 17,   |
| d to follow safe wor |   |   |   |   |         | A2;       | 19        |
| k practices such as: |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec IV    |           |
| ###### a. Hands are  |   |   |   |   |         | BLS-1 A4; |           |
| washed after work wi |   |   |   |   |         |           |           |
| th cultures, glove r |   |   |   |   |         | Sec IV    |           |
| emoval, and before e |   |   |   |   |         | BSL-1 A5; |           |
| xiting the lab area. |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec IV    |           |
| > b\. Eating,        |   |   |   |   |         | BSL-1 A6; |           |
| > drinking, smoking, |   |   |   |   |         |           |           |
| > handling contact   |   |   |   |   |         | Sec IV    |           |
| > lenses, and/or     |   |   |   |   |         | BSL-1 A7; |           |
| > applying cosmetics |   |   |   |   |         |           |           |
| > are prohibited in  |   |   |   |   |         | Sec V     |           |
| > all labs.          |   |   |   |   |         | ABSL-1    |           |
| >                    |   |   |   |   |         | A-8;      |           |
| > c\. Mouth pipeting |   |   |   |   |         |           |           |
| > is prohibited and  |   |   |   |   |         | Sec V     |           |
| > mechanical devices |   |   |   |   |         | ABSL-1    |           |
| > are used.          |   |   |   |   |         | A-9;      |           |
| >                    |   |   |   |   |         |           |           |
| > d\. Procedures     |   |   |   |   |         | Sec V     |           |
| > minimize potential |   |   |   |   |         | ABSL-1    |           |
| > splashes or        |   |   |   |   |         | A-10;     |           |
| > aerosols.          |   |   |   |   |         |           |           |
| >                    |   |   |   |   |         | Sec V     |           |
| > e\. Policies for   |   |   |   |   |         | ABSL-1    |           |
| > the safe handling  |   |   |   |   |         | A-11;     |           |
| > of sharps are      |   |   |   |   |         |           |           |
| > enforced.          |   |   |   |   |         | Sec V     |           |
| >                    |   |   |   |   |         | ABSL-1    |           |
| > f\. Lab equipment  |   |   |   |   |         | A-12;     |           |
| > and work surfaces  |   |   |   |   |         |           |           |
| > are decontaminated |   |   |   |   |         | Sec V     |           |
| > at the end of the  |   |   |   |   |         | ABSL-1    |           |
| > day and after any  |   |   |   |   |         | C.4;      |           |
| > spill or splash.   |   |   |   |   |         |           |           |
| >                    |   |   |   |   |         | NIH       |           |
| > g\. Laboratory     |   |   |   |   |         | Guideline |           |
| > cold storage is    |   |   |   |   |         | A         |           |
| > not used to store  |   |   |   |   |         | ppendices |           |
| > food items for     |   |   |   |   |         |           |           |
| > personnel.         |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-1-b |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-1-d |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -ii-A-1-e |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-1-f |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | 29 CFR    |           |
|                      |   |   |   |   |         | 1910.1450 |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | A         |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 3. Insect an  |   |   |   |   |         | BMBL Sec  | 1, 2, 6,  |
| d rodent control pro |   |   |   |   |         | IV BSL-1  | 10, 17    |
| grams are in place.  |   |   |   |   |         | A.10;     |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | A.14;     |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-2-b |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Safety Equipment   |   |   |   |   |         |           |           |
| (Primary Barriers)** |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### Lab coat      |   |   |   |   |         | BMBL Sec  | 1, 2, 6,  |
| s, gowns, or uniform |   |   |   |   |         | IV BSL-1  | 10, 17    |
| s are worn to minimi |   |   |   |   |         | C.2;      |           |
| ze contamination of  |   |   |   |   |         |           |           |
| street clothes. No o |   |   |   |   |         | Sec V     |           |
| pen-toed shoes or sa |   |   |   |   |         | ABSL-1    |           |
| ndals are permitted. |   |   |   |   |         | C.2       |           |
|                      |   |   |   |   |         |           |           |
| ######               |   |   |   |   |         | 29 CFR    |           |
|                      |   |   |   |   |         | 1910.1450 |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | A .E      |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| ####                 |   |   |   |   |         | BMBL Sec  | 1, 2, 6,  |
| ## 5. Disposable glo |   |   |   |   |         | IV BSL-1  | 10, 17    |
| ves are available -  |   |   |   |   |         | C.4;      |           |
| specific types based |   |   |   |   |         |           |           |
|  on risk assessment. |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
| ###### Reusa         |   |   |   |   |         | C.4       |           |
| ble gloves (utility, |   |   |   |   |         |           |           |
|  autoclave, cryogeni |   |   |   |   |         |           |           |
| c, etc.) may be need |   |   |   |   |         |           |           |
| ed and must be maint |   |   |   |   |         |           |           |
| ained in a clean and |   |   |   |   |         |           |           |
|  sanitary condition. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ####                 |   |   |   |   | 29 CFR  | BMBL Sec  | 1, 2, 6,  |
| ## 6. Eye protection |   |   |   |   | 19      | IV BSL-1  | 10, 17    |
|  is provided and use |   |   |   |   | 10.1030 | C.3;      |           |
| d for work that may  |   |   |   |   | (c)(3)  |           |           |
| involve splashes of  |   |   |   |   |         | Sec V     |           |
| hazardous materials. |   |   |   |   | 29 CFR  | ABSL-1    |           |
|                      |   |   |   |   | 1910.   | C.3       |           |
|                      |   |   |   |   | 1450(e) |           |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   | 29 CFR  |           |           |
|                      |   |   |   |   | 1       |           |           |
|                      |   |   |   |   | 910.132 |           |           |
|                      |   |   |   |   | and 133 |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Facilities         |   |   |   |   |         |           |           |
| (Secondary           |   |   |   |   |         |           |           |
| Barriers)**          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ######               |   |   |   |   |         | BMBL Sec  | 1, 2, 3,  |
|  7. Labs have doors. |   |   |   |   |         | IV BSL-1  | 6, 10, 17 |
|                      |   |   |   |   |         | D.1;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | D.1       |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| #####                |   |   |   |   | 29 CFR  | BMBL Sec  | 1, 2, 6,  |
| # 8. Labs/facilities |   |   |   |   | 1910.1  | IV BSL-1  | 10, 17    |
|  have a sink availab |   |   |   |   | 030(d)( | D.2;      |           |
| le for hand-washing. |   |   |   |   | 2)(iii) |           |           |
|                      |   |   |   |   | for BBP | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | D.2;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | D.3;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | D.4;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | D.5       |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-4-d |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| **E. BIOSAFETY LEVEL |   |   |   |   |         |           |           |
| (BSL) 2 AND ANIMAL   |   |   |   |   |         |           |           |
| BIOSAFETY LEVEL      |   |   |   |   |         |           |           |
| (ABSL) 2             |   |   |   |   |         |           |           |
| LABORATORIES**       |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **All standard       |   |   |   |   |         |           |           |
| microbiological      |   |   |   |   |         |           |           |
| practices, safety    |   |   |   |   |         |           |           |
| equipment, and       |   |   |   |   |         |           |           |
| facility             |   |   |   |   |         |           |           |
| requirements for     |   |   |   |   |         |           |           |
| BSL/ABSL-1 apply in  |   |   |   |   |         |           |           |
| addition to the      |   |   |   |   |         |           |           |
| following:**         |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Standard           |   |   |   |   |         |           |           |
| Microbiological      |   |   |   |   |         |           |           |
| Practices**          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| #####                |   |   |   |   |         | BMBL      | 6, 10,    |
| # 1. Lab Director en |   |   |   |   |         | BSL-2     | 12, 13,   |
| sures personnel rece |   |   |   |   |         | A.11;     | 17, 19    |
| ive initial training |   |   |   |   |         |           |           |
|  on the potential ha |   |   |   |   |         | ABSL-2    |           |
| zards of specific ag |   |   |   |   |         | A.3;      |           |
| ent(s) and annual up |   |   |   |   |         |           |           |
| dates or additional  |   |   |   |   |         | NIH       |           |
| training as needed.  |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-B-2-m |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 2. The Lab D  |   |   |   |   |         | BMBL      | 6, 10,    |
| irector ensures lab  |   |   |   |   |         | BSL-2 B.5 | 12, 17    |
| personnel are profic |   |   |   |   |         |           |           |
| ient in applicable b |   |   |   |   |         |           |           |
| iosafety procedures. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 3. Contaminat |   |   |   |   | 29 CFR  | BMBL      | 1, 2, 6,  |
| ed materials are pla |   |   |   |   | 19      | BSL-2     | 10, 17    |
| ced in properly labe |   |   |   |   | 10,1030 | B.6;      |           |
| led containers that  |   |   |   |   | (c)(    |           |           |
| prevent leakage duri |   |   |   |   | 4)(iii) | ABSL-2    |           |
| ng collection, handl |   |   |   |   | for BBP | A.15      |           |
| ing, processing, sto |   |   |   |   |         |           |           |
| rage, transport, dis |   |   |   |   |         |           |           |
| posal, and shipping. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 4. Potenti    |   |   |   |   | 29 CFR  | BMBL Sec  | 1, 2, 6,  |
| ally contaminated wa |   |   |   |   | 1       | IV BSL-1  | 10, 17    |
| ste is decontaminate |   |   |   |   | 910.103 | A.8;      |           |
| d by an approved met |   |   |   |   | 0(e)(1) |           |           |
| hod before disposal. |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   |         | ABSL-1    |           |
|                      |   |   |   |   |         | A.15;     |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-A-1-c |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | 29 CFR    |           |
|                      |   |   |   |   |         | 1910.1450 |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | A         |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Safety Equipment   |   |   |   |   |         |           |           |
| (Primary Barriers)** |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 5. BSCs ar    |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| e properly maintaine |   |   |   |   |         | BSL-2     | 10, 17    |
| d and used for lab p |   |   |   |   |         | C.1;      |           |
| rocedures involving: |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | ABSL-2    |           |
| ###### a. Aerosols   |   |   |   |   |         | C.1       |           |
| and/or splashes; or  |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NSF 49    |           |
| ###### b.            |   |   |   |   |         |           |           |
| High concentrations  |   |   |   |   |         |           |           |
| and/or large volumes |   |   |   |   |         |           |           |
|  of infectious agent |   |   |   |   |         |           |           |
| s. *Note: Current ce |   |   |   |   |         |           |           |
| rtification is a mai |   |   |   |   |         |           |           |
| ntenance indicator.* |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 6             |   |   |   |   | 29 CFR  | BMBL      | 1, 2, 6,  |
| . Appropriate person |   |   |   |   | 1910.1  | BSL-2     | 10, 17    |
| al protective equipm |   |   |   |   | 450(e)( | C.2-5;    |           |
| ent (PPE), as determ |   |   |   |   | 3)(iii) | ABSL-2    |           |
| ined by risk assessm |   |   |   |   |         | C.2-4;    |           |
| ent, is used and lef |   |   |   |   | 29 CFR  |           |           |
| t in the laboratory  |   |   |   |   | 1       | NIH       |           |
| area before exiting. |   |   |   |   | 910.132 | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-B-2-f |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| **Facilities         |   |   |   |   |         |           |           |
| (Secondary           |   |   |   |   |         |           |           |
| Barriers)**          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ######               |   |   |   |   | 29 CFR  | BMBL Sec  | 1, 2, 6,  |
|  7. When appropriate |   |   |   |   | 1910.   | IV BSL-2  | 10, 13,   |
| , biohazard signs ar |   |   |   |   | 1030(e) | A.9;      | 17        |
| e posted with the fo |   |   |   |   | & (g)   |           |           |
| llowing information: |   |   |   |   |         | Sec V     |           |
|                      |   |   |   |   | 29 CFR  | ABSL-2    |           |
| ##                   |   |   |   |   | 1       | A.5       |           |
| #### Agent(s) in Use |   |   |   |   | 910.145 |           |           |
|                      |   |   |   |   |         |           |           |
| ##                   |   |   |   |   |         |           |           |
| #### Biosafety Level |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| ###### PPE and Immun |   |   |   |   |         |           |           |
| ization requirements |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| ###### PI and Lab Su |   |   |   |   |         |           |           |
| pervisor's Name with |   |   |   |   |         |           |           |
|  contact information |   |   |   |   |         |           |           |
|                      |   |   |   |   |         |           |           |
| ###### Entry         |   |   |   |   |         |           |           |
|  and Exit procedures |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 8. Lab        |   |   |   |   |         | BMBL      | 1, 2, 3,  |
|  doors are self-clos |   |   |   |   |         | BSL-2     | 6, 10, 17 |
| ing with locks that  |   |   |   |   |         | D.1;      |           |
| conform to local ins |   |   |   |   |         |           |           |
| titutional policies. |   |   |   |   |         | ABSL-2    |           |
|                      |   |   |   |   |         | D.1       |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NFPA      |           |
|                      |   |   |   |   |         | 101-      |           |
|                      |   |   |   |   |         | 7.2.1.8.1 |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ######               |   |   |   |   |         | BMBL      | 1, 2, 6,  |
|  9. Lab is construct |   |   |   |   |         | BSL-2     | 10, 17    |
| ed and equipped to f |   |   |   |   |         | D.3-4;    |           |
| acilitate cleaning a |   |   |   |   |         | ABSL-2    |           |
| nd decontamination.  |   |   |   |   |         | D.3-4     |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 10.           |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| BSCs are located awa |   |   |   |   |         | BSL-2     | 10, 17    |
| y from doors, window |   |   |   |   |         | D.6;      |           |
| s that open, and hea |   |   |   |   |         |           |           |
| vily traveled areas. |   |   |   |   |         | ABSL-2    |           |
|                      |   |   |   |   |         | D.11      |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 11. Adequat   |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| e illumination is pr |   |   |   |   |         | A         | 10, 17    |
| ovided to minimize r |   |   |   |   |         | BSL2.D.10 |           |
| eflections and glare |   |   |   |   |         |           |           |
|  that impede vision. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ####                 |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| ## 12. Bench tops ar |   |   |   |   |         | BSL-2     | 10, 17    |
| e impervious to wate |   |   |   |   |         | D.4a.;    |           |
| r and resistant to d |   |   |   |   |         | ABSL-2    |           |
| amage by moderate he |   |   |   |   |         | D.4;      |           |
| at and/or chemicals. |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-B-4-b |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 13. Lab fu    |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| rniture is covered w |   |   |   |   |         | BSL-2     | 10, 17    |
| ith a non-fabric mat |   |   |   |   |         | D.4b;     |           |
| erial that can easil |   |   |   |   |         |           |           |
| y be decontaminated. |   |   |   |   |         | ABSL-2    |           |
|                      |   |   |   |   |         | D.4       |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| **Lab Specific       |   |   |   |   |         |           |           |
| Practices**          |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 14. Lab       |   |   |   |   |         | BMBL      | 3, 6, 10, |
|  Director (PI if non |   |   |   |   |         | BSL-2     | 17        |
| exempt rDNA) is resp |   |   |   |   |         | B.1;      |           |
| onsible for assessin |   |   |   |   |         |           |           |
| g lab-associated ris |   |   |   |   |         | ABSL-2    |           |
| k(s) and establishin |   |   |   |   |         | A.6;      |           |
| g entry/exit require |   |   |   |   |         |           |           |
| ments for personnel. |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G-        |           |
|                      |   |   |   |   |         | II-B-1-a; |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | B-2-b;    |           |
|                      |   |   |   |   |         | B-2-c.    |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| #####                |   |   |   |   |         | BMBL      | 6, 10, 17 |
| # 15. Lab Director i |   |   |   |   |         | BSL-2     |           |
| ncorporates biosafet |   |   |   |   |         | B.4;      |           |
| y procedures into la |   |   |   |   |         |           |           |
| b SOPs or utilizes a |   |   |   |   |         | ABSL-2    |           |
|  lab-specific resear |   |   |   |   |         | A.2;      |           |
| ch biosafety manual. |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-B-2-m |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

+----------------------+---+---+---+---+---------+-----------+-----------+
| ######               |   |   |   |   |         | BMBL      | 1, 2, 4,  |
|  16. Lab personnel r |   |   |   |   |         | BSL-2     | 6, 10, 17 |
| eceive appropriate i |   |   |   |   |         | B.2;      |           |
| mmunizations and/or  |   |   |   |   |         |           |           |
| clinical testing for |   |   |   |   |         | ABSL-2    |           |
|  agents of concern.  |   |   |   |   |         | B.1       |           |
|                      |   |   |   |   |         |           |           |
| ###### *Note         |   |   |   |   |         |           |           |
| : Medical surveillan |   |   |   |   |         |           |           |
| ce program is based  |   |   |   |   |         |           |           |
| on risk assessment.* |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| #                    |   |   |   |   |         | BMBL      | 1, 2, 4,  |
| ##### 17. Baseline s |   |   |   |   |         | BSL-2     | 6, 10, 17 |
| erum samples are col |   |   |   |   |         | B.3;      |           |
| lected and stored fo |   |   |   |   |         |           |           |
| r all at-risk person |   |   |   |   |         | ABSL-2    |           |
| nel, as appropriate. |   |   |   |   |         | B.1;      |           |
|                      |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | uidelines |           |
|                      |   |   |   |   |         | Appendix  |           |
|                      |   |   |   |   |         | G         |           |
|                      |   |   |   |   |         | -II-B-2-l |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 18. The l     |   |   |   |   | 29 CFR  | BMBL      | 1, 2, 6,  |
| ab and lab equipment |   |   |   |   | 1910.1  | BSL-2     | 10, 17    |
|  are routinely decon |   |   |   |   | 030(c)( | B.7;      |           |
| taminated in accorda |   |   |   |   | 2)(xiv) |           |           |
| nce with applicable  |   |   |   |   | for BBP | ABSL-2    |           |
| local, state and/or  |   |   |   |   |         | B.4       |           |
| federal regulations  |   |   |   |   | 29 CFR  |           |           |
| and articles are con |   |   |   |   | 1910.14 |           |           |
| tained or decontamin |   |   |   |   | 50(e)(3 |           |           |
| ated prior to remova |   |   |   |   | )(viii) |           |           |
| l from the facility. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###### 19. Incid     |   |   |   |   |         | BMBL      | 1, 2, 4,  |
| ents involving infec |   |   |   |   |         | BSL-2     | 6, 10, 17 |
| tious materials are: |   |   |   |   |         | B.8;      |           |
|                      |   |   |   |   |         |           |           |
| ###### R             |   |   |   |   |         | ABSL-2    |           |
| eported immediately  |   |   |   |   |         | B.5;      |           |
| to the Lab Director; |   |   |   |   |         |           |           |
|                      |   |   |   |   |         | NIH       |           |
| ####                 |   |   |   |   |         | G         |           |
| ## Followed by medic |   |   |   |   |         | uidelines |           |
| al monitoring and ap |   |   |   |   |         | Appendix  |           |
| propriate care of pe |   |   |   |   |         | G         |           |
| rsonnel at risk; and |   |   |   |   |         | -II-B-2-k |           |
|                      |   |   |   |   |         |           |           |
| ###### D             |   |   |   |   |         |           |           |
| ocumented by a writt |   |   |   |   |         |           |           |
| en report or record. |   |   |   |   |         |           |           |
+----------------------+---+---+---+---+---------+-----------+-----------+
| ###                  |   |   |   |   |         | BMBL      | 1, 2, 6,  |
| ### 20. Animals and  |   |   |   |   |         | BSL-2     | 10, 17    |
| plants not involved  |   |   |   |   |         | B.9;      |           |
| in research cannot e |   |   |   |   |         |           |           |
| nter the laboratory. |   |   |   |   |         | ABSL-2    |           |
|                      |   |   |   |   |         | A.13      |           |
+----------------------+---+---+---+---+---------+-----------+-----------+

<table style="width:100%;">
<colgroup>
<col style="width: 32%" />
<col style="width: 3%" />
<col style="width: 0%" />
<col style="width: 3%" />
<col style="width: 5%" />
<col style="width: 0%" />
<col style="width: 3%" />
<col style="width: 0%" />
<col style="width: 14%" />
<col style="width: 17%" />
<col style="width: 17%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="11"><strong>F. BIOSAFETY LEVEL (BSL) 3 AND ANIMAL BIOSAFETY
LEVEL (ABSL) 3 LABORATORIES</strong></td>
</tr>
<tr class="even">
<td colspan="11"><strong>All standard microbiological practices, safety
equipment, and facility requirements for BSL/ABSL-1 and BSL/ABSL-2 apply
in addition to the following:</strong></td>
</tr>
<tr class="odd">
<td colspan="11"><strong>Standard Microbiological
Practices</strong></td>
</tr>
<tr class="even">
<td><h6
id="facility-has-methods-to-decontaminate-all-infectious-lab-waste---preferably-within-the-lab.">1.
Facility has method(s) to decontaminate all infectious lab waste -
preferably within the lab.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td><p>BMBL BSL-3 D.11;</p>
<p>ABSL-3 B.5;</p>
<p>ABSL-3 D.12;</p>
<p>NIH Guidelines Appendix G-II-C-4-h</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td><h6
id="materials-for-decontamination-outside-the-lab-are-packaged-in-accordance-with-applicable-local-state-and-federal-regulations-before-removal.">2.
Materials for decontamination outside the lab are packaged in accordance
with applicable local, state, and federal regulations before
removal.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2">DOT HM 181 (Hazardous Materials Regulations)</td>
<td><p>BMBL BSL-3 A.8b;</p>
<p>NIH Guidelines Appendix G-II-C-2-b</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td colspan="11"><strong>Safety Equipment (Primary
Barriers)</strong></td>
</tr>
<tr class="odd">
<td><h6
id="standard-ppe-includes-solid-front-lab-coats-wrap-around-gowns-or-coveralls-and-reusable-ppe-is-decontaminated-before-laundering.">3.
Standard PPE includes solid front lab coats, wrap-around gowns, or
coveralls and reusable PPE is decontaminated before
laundering.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td><p>BMBL BSL-3 C.2;</p>
<p>ABSL-3 C.2</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="gloves-are-worn-when-handling-infectious-agents-animals-andor-contaminated-equipment-and-disposed-after-use-when-overtly-contaminated-or-when-the-integrity-is-compromised.">4.
Gloves are worn when handling infectious agents, animals, and/or
contaminated equipment and disposed after use, when overtly
contaminated, or when the integrity is compromised.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td><p>BMBL BSL-3 C.4;</p>
<p>ABSL-3 C.4</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td><h6
id="properly-selected-and-fit-tested-respiratory-andor-face-protection-is-used-in-rooms-with-infected-animals.-employees-wearing-respiratory-protection-receive-required-training.">5.
Properly selected and fit tested respiratory and/or face protection is
used in rooms with infected animals. Employees wearing respiratory
protection receive required training.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td><p>BMBL BSL-3 C.5;</p>
<p>ABSL-3 C.3</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="manipulations-of-infected-materials-are-conducted-in-class-ii-or-iii-bsc.-bsc-or-other-physical-containment-devices-are-used-for-procedures-that-may-produce-aerosols.">6.
Manipulations of infected material(s) are conducted in Class II or III
BSC. BSC or other physical containment devices are used for procedures
that may produce aerosols.</h6></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td><p>BMBL BSL-3 C.1;</p>
<p>ABSL-3 C.1;</p>
<p>NIH Guidelines Appendix G-II-C-3-a</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td colspan="11"><strong>Facilities (Secondary Barriers)</strong></td>
</tr>
<tr class="even">
<td><h6
id="lab-areas-are-separated-from-general-building-traffic-and-entered-through-an-anteroom-separated-by-2-self-closing-and-locking-doors.">7.
Lab areas are separated from general building traffic and entered
through an anteroom separated by 2 self-closing and locking
doors.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 D.1;</p>
<p>ABSL-3 D.1</p></td>
<td>1, 2, 3, 6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td><h6
id="each-lab-contains-a-hands-free-sink-for-hand-washing-near-the-exit.-if-the-lab-has-segregated-zones-each-must-have-a-sink.">8.
Each lab contains a hands-free sink for hand-washing near the exit. If
the lab has segregated zones, each must have a sink.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 D.2;</p>
<p>ABSL-3 D.2;</p>
<p>NIH Guidelines Appendix G-II-C-4-e</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="an-eyewash-station-is-available-and-located-in-the-laboratory.">9.
An eyewash station is available and located in the laboratory.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td>BMBL BSL-3 D.13;</td>
<td></td>
</tr>
<tr class="odd">
<td><h6
id="the-laboratory-must-be-designed-so-that-it-can-be-easily-cleaned-and-decontaminated.-all-windows-are-sealed.-carpets-and-rugs-are-not-permitted.-seams-floors-walls-and-ceiling-surfaces-should-be-sealed.-spaces-around-doors-and-ventilation-openings-should-be-capable-of-being-sealed-to-facilitate-space-decontamination.">10.
The laboratory must be designed so that it can be easily cleaned and
decontaminated. All windows are sealed. Carpets and rugs are not
permitted. Seams, floors, walls, and ceiling surfaces should be sealed.
Spaces around doors and ventilation openings should be capable of being
sealed to facilitate space decontamination.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>VHA Handbook 1200.06.7.e;</p>
<p>BMBL BSL-3 D.5;</p>
<p>ABSL-3 D.5;</p>
<p>NIH Guidelines Appendix G-II-C-4-f</p></td>
<td>1, 2, 3, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="vacuum-lines-are-protected-by-disinfectant-traps-and-hepa-filters-or-equivalent.">11.
Vacuum lines are protected by disinfectant traps and HEPA filters or
equivalent.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 D.7;</p>
<p>NIH Guidelines Appendix G-II-C-2-o</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td><h6
id="facility-management-or-engineering-services-documents-and-verifies-bsl-3-facility-design-procedures-and-function-prior-to-operation-and-annually-at-minimum.">12.
Facility Management or Engineering Services documents and verifies BSL-3
facility design, procedures, and function prior to operation and
annually at minimum.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 D.15;</p>
<p>ABSL-3 D.14</p></td>
<td>1, 2, 6, 10, 15, 17, 18</td>
</tr>
<tr class="even">
<td><h6 id="lab-specific-practices">Lab Specific Practices</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td><h6
id="all-personnel-entering-the-lab-are-advised-of-potential-hazards-and-meet-specific-entryexit-requirements-limited-access.">13.
All personnel entering the lab are advised of potential hazards and meet
specific entry/exit requirements (limited access).</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 B.1;</p>
<p>ABSL-3 A.6;</p>
<p>NIH Guidelines Appendix G-II-C-2-d</p></td>
<td>3, 6, 10, 12, 15, 16, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="all-personnel-receive-appropriate-medical-evaluation-surveillance-and-treatment-as-documented-by-written-records.">14.
All personnel receive appropriate medical evaluation, surveillance, and
treatment as documented by written records.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 B.2;</p>
<p>ABSL-3 A.4</p></td>
<td>4, 6, 10, 13, 15, 17, 18, 19</td>
</tr>
<tr class="odd">
<td><h6
id="the-lab-director-develops-a-lab-specific-biosafety-manual-to-address-specific-hazards-and-ensures-laboratory-personnel-have-read-and-proficiently-follow-specific-practices-and-procedures.">15.
The Lab Director develops a lab-specific biosafety manual to address
specific hazards, and ensures laboratory personnel have read and
proficiently follow specific practices and procedures.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 B.4;</p>
<p>ABSL-3 A.2;</p>
<p>NIH Guidelines Appendix G-II-C-2-s</p></td>
<td>6, 10, 12, 13, 15, 16, 17, 18</td>
</tr>
<tr class="even">
<td><h6
id="equipment-and-work-surfaces-are-disinfected-regularly-after-work-wagents-andor-after-spills-or-contamination-have-occurred.">16.
Equipment and work surfaces are disinfected regularly, after work
w/agents, and/or after spills or contamination have occurred.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 B.7;</p>
<p>ABSL-3 A.12</p></td>
<td>6, 10, 15, 17, 18</td>
</tr>
<tr class="odd">
<td><h6
id="spills-and-accidents-involving-infectious-materials-are-immediately-reported-to-the-lab-director-facility-safety-officer-project-officer-and-facility-director.">17.
Spills and accidents involving infectious materials are immediately
reported to the Lab Director, Facility Safety Officer, Project Officer,
and Facility Director.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>BMBL BSL-3 B.8;</p>
<p>ABSL-3 B.7;</p>
<p>NIH Guidelines Appendix G-II-C-2-q</p></td>
<td>3, 4, 6, 10, 13, 15, 17, 18, 19</td>
</tr>
<tr class="even">
<td><h6 id="the-srs-reviews-bsl-3-sops-annually.">18. The SRS reviews
BSL-3 SOPs annually.</h6></td>
<td></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td colspan="2"></td>
<td></td>
<td><p>VHA Handbook</p>
<p>1200.06.7.a.8</p></td>
<td>6, 10, 15, 16, 17, 18, 22</td>
</tr>
</tbody>
</table>

  ---------- ------------------------------ -- ------- --------------------------
  ABSL       Animal Biosafety Level            NEC     National Electrical Code

  ACOS/R&D   Associate Chief of Staff --       NFPA    National Fire Prevention
             Research and Development                  Association

  AGA        American Gas Association          NHPP    Nuclear Health Physics
                                                       Program

  APHIS      Animal and Plant Health           NIH     National Institutes of
             Inspection Service (USDA)                 Health (DHHS)

  ASISTS     Automated Safety Incident         NSF     National Sanitation
             Surveillance and Tracking                 Foundation
             System                                    

  AWE        Annual Workplace Evaluation       NRC     U.S. Nuclear Regulatory
                                                       Commission

  BMBL       *Biosafety in Microbiological     OIG     Office of Inspector
             and Biomedical Laboratories,              General
             5^th^ Edition*                            

  BSC        Biological Safety Cabinet         ORD     Office of Research and
                                                       Development

  BSL        Biosafety Level                   OSH     Occupational Safety and
                                                       Health

  BSO        Biosafety Officer                 OSHA    Occupational Safety and
                                                       Health Administration

  CFR        Code of Federal Regulations       OSHP    Occupational Safety and
                                                       Health Program

  CHO        Chemical Hygiene Officer          PI      Principal Investigator

  CDC        Centers for Disease Control       PPE     Personal Protective
             and Prevention (DHHS)                     Equipment

  CRADO      Chief of Research and             RadSC   Radiation Safety Committee
             Development Officer                       

  DHHS       U.S. Department of Health and     R&D     Research and Development
             Human Services                            Committee

  DOT        U.S. Department of                rDNA    recombinant DNA
             Transportation                            (deoxyribonucleic acid)

  EPA        U.S. Environmental Protection     RSC     Research Safety
             Agency                                    Coordinator

  EPRP       Emergency Preparedness and        RSO     Radiation Safety Officer
             Response Plan                             

  GFI        ground fault interrupter          RSP     Research Safety Program

  HEPA       High Efficiency Particulate       SAFE    Safety Automated Facility
             Air                                       Evaluation

  IBC        Institutional Biosafety           SOPs    Standard Operating
             Committee                                 Procedures

  L          liter                             SRS     Subcommittee on Research
                                                       Safety

  MCD        Medical Center Director           VA      U.S. Department of
                                                       Veterans' Affairs

  MOU        Memorandum of Understanding       VHA     Veterans Health
                                                       Administration

  NHPP       Nuclear Health Physics Program    VISN    Veterans Integrated
                                                       Service Network

  MSDS       Material Safety Data Sheets       USDA    U.S. Department of
                                                       Agriculture
  ---------- ------------------------------ -- ------- --------------------------