**CURRICULUM VITAE**

Dale E. A. Lewis

NIH/NCI/CCR

Laboratory of Molecular Biology

Developmental Genetics Section

Building 37, Room 5138

National Cancer Institute

37 Convent Drive

Bethesda, MD 20892-4264

Phone: 301-496-9129

Fax: 301-496-2212

E-Mail: [aed@helix.nih.gov]{.underline}

**Citizenship:** United States

**Education:**

1984 - 1986 A.A.S., Medical Laboratory Technology, Bronx Community
College,

Bronx, NY

1986 - 1989 B.S., Biochemistry, The City College of New York, New York,
NY

1989 - 1992 M.S., Molecular Biology, Princeton University, NJ

1992 - 1995 Ph.D., Molecular Biology, Princeton University, NJ

**Chronology of Employment:**

2002 - present Staff Scientist, Developmental Genetics Section,
Laboratory of

Molecular Biology, NCI, NIH

2000 - 2002 Research Fellow, Developmental Genetics Section, Laboratory
of

Molecular Biology, NCI, NIH

1995 - 2000 Postdoctoral Fellow, Developmental Genetics Section,
Laboratory of

Molecular Biology, National Cancer Institute (NCI), National Institutes

1990 - 1995 Predoctoral Student, Princeton University, Princeton, NJ

of Health (NIH), Bethesda, MD

1993 Visiting Scientist, University of Madison-Wisconsin, Madison, WI

1992 Visiting Scientist, University of Leeds, Leeds, England LS2 9JT

1990 Rotation Student, Princeton University, Princeton, NJ

1986 -1989 Research Aide, The City College of New York, New York, NY

1988 Research Assistant, University of Pennsylvania, Philadelphia, PA

1986 Research Assistant, Bronx Community College, Bronx, NY

**Societies/Committees:**

American Society for Microbiology

NIH Occupant Emergency Organization

**Honors and other special Scientific Recognition:**

1995 Citation of Merit presented by the Bronx Borough President:
Fernando Ferrer, Bronx, NY

1989 -- 1995 Minority Access to Research Careers Predoctoral Fellowship,
Princeton University, Princeton, NJ

1989 Graduate with Cum Laude Honors, The City College of New York, NY

1989 American Institute of Chemists Award

1988 Participant, Biomedical Graduate Summer Program, University of

Pennsylvania, Philadelphia, PA

1988 Dean\'s List, The City College of New York, New York, NY

1987 Participant, National Aeronautics Space Administration\'s Space
Life

Sciences Training Program, Kennedy Space Center, Cape Canaveral, Fl

1986 -- 1988 Minority Access to Research Careers Fellowship, The City
College of New York, NY

1986 Volunteer Service, Mount Sinai Medical Center, New York, NY

1986 Commencement Scholarship Award in Chemistry in Medical

Laboratory Technology, Bronx Community College, Bronx, NY

1985 - 1986 Student Government Service Award at Bronx Community College

1985 Elected to Phi Theta Kappa, National Honor Fraternity

1984-1986 National Dean\'s List,

1984 - 1986 Dean\'s List at Bronx Community College, Bronx, NY,

**Student Mentoring:**

1994 Michael Beck

Role of Amino-terminal Arms in the Tandem Binding of TrpR to DNA

Princeton University, Princeton, NJ 08544

1997 Vinay Chattoraj

> Construction of Plasmids and Phages to Study DNA Looping
>
> Mechanism of the *gal* Operon

National Cancer Institute, Bethesda, MD 20892

1997 - 1999 Angela Fox **(**Pre-Doc CRTA)

> Analysis of Transcription Bubble at *galP1* in the Presence and
>
> Absence of cAMP-CRP Complex.

National Cancer Institute, Bethesda, MD 20892

1999 - 2000 James Thomas

> Investigating the Degree of Superhelicity in the Domains of *E. coli*
> by
>
> Using DNA Transposons
>
> National Cancer Institute, Bethesda, MD 20892

2002 Elizabeth Scheimer

How Do Regulators Activate Promoters: Contact Between Regulators.

National Cancer Institute, Bethesda, MD 20892

**Technician Supervised:**

2004 - present Phuoc Le

**Presentations**

Lewis, D. E. A. and Adhya, S.

Requirement of Specific Upstream DNA Sequence for Blocking Elongation
Complexes by Operator-Bound GalR.

Post-initiation Activities of RNA Polymerases Meeting, Mountain Lake,
VA; 2006

Lewis, D. E. A. and Adhya, S.

Requirement of Specific Upstream DNA Sequence for Blocking Elongation
Complexes by Operator-Bound GalR.

Molecular Genetics of Bacteria & Phages, Cold Spring Harbor, NY; 2006

Lewis, D. E. A. and Adhya, S.

DNA Looping and Transcription Repression in Bacteriophage λ.

Systems Biology of Phage λ, Dragor, Denmark, 2006

Lewis, D. E. A. and Adhya, S.

Requirement of Specific Upstream DNA Sequence for Blocking Elongation
Complexes by Operator-Bound GalR.

2^nd^ Annual CCR Staff Scientist and Staff Clinician Retreat,
Gaithersburg, MD, 2006

Lewis, D. E. A. and Adhya, S.

Requirement of Specific Upstream DNA Sequence for Blocking Elongation
Complexes by Operator-Bound GalR.

American Society for Microbiology, Atlanta, GA, 2005

*Finzi, L.*, Lia, G., Dunlap, D., Nelson, P., Lewis, D. E. A. , Adhya,
S.

# Probing regulation of gene transcription by single molecule analysis

Human Frontier Science Program, Paris,France 2005

Lewis, D. E. A.

Transcription Activation by cAMP Receptor Protein (CRP) at the
*Escherichia coli galP1* Promoter: Crucial Role for the Spacing Between
the CRP Binding Site and the -10 Region.

Universitá Statale di Milano, Italy, 2004

Lewis, D. E. A. and Adhya, S.

*In Vitro* Repression of the *gal* Promoters by GalR and HU Depends on
the Proper Helical Phasing of the Two Operators.

American Society for Microbiology, Salt Lake City, UT; 2002

Lewis, D. E. A. and Adhya, S.

*In Vitro* Repression of the *gal* Promoters by GalR and HU Depends on
the Proper Helical Phasing of the Two Operators.

2nd Annual Postdoctoral Retreat of NCI, Baltimore, MD; 2002

Lewis, D. E. A., Geanacopoulos, M., Vasmatzis, G. and Adhya, S.

Repressosome: A DNA-Multiprotein Complex Involved in the Repression of
the *gal*

Promoters.

Molecular Genetics of Bacteria and Phages, Cold Spring Harbor, NY; 1998

Lewis, D. E. A., Geanacopoulos, M., Aki, T. and Adhya, S.

DNA Looping Mediated Repression by GalR Needs HU and Supercoiled DNA.

Molecular Genetics of Bacteria and Phages, Madison, Wisconsin; 1997

Lewis, D. E. A., Carey, J., Lawson, C. L. and Royer, C. A.

Tandem Binding of *E. coli* Trytophan Repressor to DNA.

National Institute of General Medical Sciences Minority Programs
Symposium, Atlanta, GA; 1993

Carey, J., Lavoie, T., Lewis, D. E. A., and Yang, J.

How Does Tryptophan Repressor Recognize its Operator?

Seventh Conversation in Biomolecular Stereodynamics, Albany, NY; 1993

Lewis, D. E. A., Morris, J. and Schulz, H.

Identification of Fatty Acid Binding Protein in Bovine Adrenal Glands,
Rat Spleens and Rat Pancreas.

Minority Access to Research Careers Symposium, Bethesda, MD; 1988

Lewis, D. E. A., Wilde, M. and Kopf. G.

Signal Transduction in Mouse Sperm.

Biomedical Graduate Program, University of Pennsylvania, Philadelphia,
PA; 1988

**Publications:**

Lewis, D. E. A., Le, P., and Adhya, S.

Determination of the Role of Individual Base Pairs in Transcription
Initiation of two Interspersed *gal* Promoters (in preparation)

Lewis, D. E. A. Komissarova, N, Le, P., Kashlev, M. and Adhya, S.

DNA sequences in *gal* operon modulate RNA polymerase elongation and
backtracking (Submitted)

[Zurla, C., Franzini, A., Galli, G., Dunlap, D. D., Lewis, D. E. A.,
Adhya, S, and Finzi, L.]{.underline}

Novel Tethered Particle Motion Analysis of CI Protein-Mediated DNA
Looping in the Regulation of Bacteriophage Lambda.

*J. Phys.: Condens. Matter* 18, S225-S234, 2006. \[Journal\]

[Lewis, D. E. A. and Adhya S]{.underline}

Axiom of Determining Transcription Start Points by RNA Polymerase in
*Escherichia coli*.

*Mol. Microbiology* 54, 692-701, 2004. \[Journal\]

[Lia, G., Bensimon, D., Croquette, V., Allenmand, J-F., Dunlap, D.,
Lewis, D. E.]{.underline}

[A., Adhya, S. and Finzi, L.]{.underline}

Supercoiling and Denaturation in Gal Repressor/Heat Unstable Nucleoid
Protein (HU)-Mediated DNA Looping.

*Proc. Natl. Acad Sci USA* 100, 11373-11377, 2003. \[Journal\]

[Lim, H. M., Lewis, D. E. A., Lee, H. J., and Adhya, S.]{.underline}

Effects of varying the Supercoiling of DNA on Transcription and its
Regulation.

*Biochemistry* 42, 10718-10725, 2003. \[Journal\]

[Lewis, D. E. A.]{.underline}

Identification of Promoters of *Escherichia coli* and Phage in
Transcription Section Plasmid pSA850.

*Methods of Enzymology* 370: 618-645, 2003. \[Journal\]

[Semsey, S., Geanacopoulos, M., Lewis, D. E. A. and Adhya,
S.]{.underline}

Operator-bound GalR Dimers Close DNA Loops by Direct Interaction
Tetramerization

and Inducer Binding.

*EMBO* 21: 1-8, 2002. \[Journal\]

[Lewis, D. E. A. and Adhya, S.]{.underline}

*In vitro* Repression of the *gal* Promoters by GalR and HU Depends on
the Proper Helical Phasing of the Two Operators.

*J. Biol. Chem.* 277: 2498-2504, 2002. \[Journal\]

[Lewis, D. E. A. and Adhya, S.]{.underline}

DNA Looping and Transcription Regulation.

*Encyclopedia of Life Science. [www.els.net](http://www.els.net/),*
2001.

[Geanacopoulos, M., Vasmatzis, G., Lewis, D. E. A., Roy, S., Lee, B.K.
and Adhya, S.]{.underline}

Genetic Analysis of the *gal* Repressosome.

*Genes Dev*. 13: 1251-1262, 1999. \[Journal\]

[Lewis, D. E. A., Geanacopoulos, M. and Adhya, S.]{.underline}

Role of HU and DNA Supercoiling in Transcription in Repression:
Specialized Nucleoprotein Repression Complex at *gal* Promoters in
*Escherichia coli*.

*Mol. Microbiol.* 3: 451-461, 1999. \[Journal\]

[Adhya, S., Geanacopoulos, M., Lewis, D. E. A., Roy, S. and Aki,
T.]{.underline}

Transcription regulation by (i) Repressosome and (ii) RNA Polymerase
Contact.

*Cold Spring Harbor Symp. Quant. Biol.* 63: 1-9, 1998. \[Journal\]

[Yang, J., Gunasekera, A., Lavoie, T. A., Jin, L., Lewis, D. E. A. and
Carey, J.]{.underline}

*In vivo* and *in vitro* Studies of TrpR-DNA Interactions.

*J. Mol. Biol.* 258: 37-52, 1996. \[Journal\]

[Carey, J., Combatti, N., Lewis, D. E. A. and Lawson, C. L.]{.underline}

Cocrystals of *E. coli* Trp Repressor Bound to an Alternative Operator
DNA Sequence.

*J. Mol. Biol.* 234: 496-498, 1993. \[Journal\]

[Lewis, D. E. A. and Carey, J.]{.underline}

Analysis of Trp Repressor-DNA Interactions Using Gel Electrophoresis.

*Electrophoresis* 14: 713-719,1993. \[Journal\]

[Carey, J., Lewis, D. E. A., Lavoie, T. and Yang, J.]{.underline}

How Does Trp Repressor Bind to Its Operator?

*J. Biol. Chem.* 266: 24509-24513, 1991. \[Journal\]