**James Sumner**

1612 Greenview Dr. (734) 945-6930

Ann Arbor, MI 48103 sumnerj@umich.edu

**Education**

> **Ph.D. Analytical Chemistry** Aug 1999 -- Present
>
> *University of Michigan,* Ann Arbor, MI GPA: 7.0/8.0
>
> **B.S. Chemistry; ACS concentration** Aug 1995 -- May 1999
>
> *James Madison University,* Harrisonburg, VA Summa Cum Laude
>
> GPA: 7.8/8.0

**Experience**

**Dr. Raoul Kopelman Laboratory**, University of Michigan Aug 1999 --
Present

*Metal-ion Nanosensor Development for Biologically Relevant
Applications*

> Designed, synthesized, and characterized ratiometric fluorescent
> nanosensors for the intracellular detection of Fe^3+^, Cu^2+^ and
> Zn^2+^. Used multiple platforms including sol-gel, liquid polymer, and
> hydro-gel matrices in sensor development. Selection, development and
> optimization of appropriate enzyme or protein based detection schemes.
> Intense outside collaboration. Knowledge of fluorescence microscopy,
> flourometry, signal optimization, interference elimination, basic
> organic synthesis, and enzymatic pathways.
>
> **Dr. Mark Meyerehoff Laboratory**, University of Michigan Jun 1999 --
> Aug 1999
>
> *Characterization of Heparin-Sensitive Electrodes and Heparin
> Releasing Polymers*

Synthesized and characterized electrodes for the detection of heparin.
Synthesized and characterized heparin releasing polymers. Knowledge of
electrochemical methods, polymer synthesis and characterization
techniques. Basic knowledge of biological pathways involving heparin,
and basic analytical theory.

**Dr. Thomas DeVore Laboratory**, James Madison University May 1997 --
May 1999

*FT-IR Evolved Gas Analysis*

Monitored and analyzed the remediation of chlorofluorocarbons by various
oxide compounds. Calculated activation energies and rates of various
reactions. Thorough knowledge of FT-IR and Differential Scanning
Calorimetry. Summers supported by NSF-REU programs.

**Instrumentation/Techniques**

+-----------------------------------------------------------------------+
| +---------------------+-----------------------+------------------+    |
| | -   Fluorescence    | -   Atomic Force      | -   NMR          |    |
| |     Spectroscopy    |     Microscopy        |                  |    |
| +---------------------+-----------------------+------------------+    |
| | -   Fluorescence    | -   Electrochemical   | -   ICP-MS       |    |
| |     Microscopy      |     instruments       |                  |    |
| +---------------------+-----------------------+------------------+    |
| | -   UV-Vis          | -   Chromtography     | -   Atomic       |    |
| |     Spectroscopy    |     (GC, HPLC, GC-MS) |     Absorption   |    |
| |                     |                       |     Spectroscopy |    |
| | -   SEM             | -   Differential      |                  |    |
| |                     |     Scanning          |                  |    |
| | -   Multiangle      |     Calorimetry       |                  |    |
| |     light           |                       |                  |    |
| |     scattering      |                       |                  |    |
| +---------------------+-----------------------+------------------+    |
+-----------------------------------------------------------------------+

James Sumner

**Teaching**

> **Graduate Student Instructor** Aug 1999 -- May 2000

University of Michigan

# General Chemistry Discussion 

Prepared weekly lesson plans. Led interactive learning sessions.
Clarified difficult concepts. Tutored students individually. Graded
assignments and exams. Suggested final grades.

> **Introductory Organic Chemistry Laboratory**

Prepared weekly lesson plans. Introduced concepts behind experimental
methods. Clarified difficult concepts. Supervised students in lab.
Oversaw lab safety. Tutored students individually. Graded assignments
and exams. Suggested final grades.

> **Teaching Assistant** Aug 1996 -- May 1999
>
> James Madison University
>
> **General Chemistry and Analytical Chemistry Laboratory**
>
> Supervised students in lab. Instructed students on experiments.
> Organized and distributed chemicals and supplies. Oversaw lab safety.

**Publications**

1.  Sumner, J. P., Kopelman, R., *Alexa Fluor 488 as an Iron Sensing
    Molecule and Its Application in PEBBLE Nanosensors, Analyst,*
    **2005**, 528-533**.**

2.  Sumner, J. P., Westerberg, N. M., Stoddard A. K, Fierke, C. A.,
    Kopelman, R., C*u^+^ and Cu^2+^ Sensitive PEBBLE Fluorescent
    Nanosensors using DsRed as the Recognition Element*, *Sensor and
    Actuators B (Submitted),* **2005.**

3.  Sumner, J. P., Westerberg, N. M., Stoddard A. K, Cramer, M.
    Thompson, R. B., Fierke, C. A., Kopelman, R., *DsRed as a Highly
    Sensitive, Selective, and Reversible Fluorescence-Based Biosensor
    for Both Cu^+^ and Cu^2+^ Ions*, Biosensors and Bioelectronics *(In
    Press),* **2005.**

4.  Brasuel, M.; Kopelman, R.; Philbert, M. A.; Aylott, J. W.; Clark,
    H.; Kasman, I.; King, M.; Monson, E.; Sumner, J.; Hoyer, M.;
    Miller, T. J.; Tjalkens, R. *PEBBLE nanosensors for real time
    intracellular chemical imaging. Optical Biosensors* **2002**,
    497-536.

5.  Sumner, J. P.; Aylott, J. W.; Monson, E.; Kopelman, R. *A
    fluorescent PEBBLE nanosensor for intracellular free zinc. Analyst
    (Cambridge, United Kingdom)* **2002**, *127*, 11-16.

6.  Sumner, J.P. and T.C. DeVore, *The reaction between
    1,2-dichloroethane and the vanadium (III, IV, V) oxides.*
    Proceedings - Electrochemical Society, **2001**, 2001-12(High
    Temperature Corrosion and Materials Chemistry III), 296-308.

7.  Parrett, J. W., Jr.; Sumner, J. P.; DeVore, T. C. *Reaction between
    chlorocarbon vapors and sodium carbonate. Environmental Science and
    Technology* **1999**, *33*, 1691-1696.

**Conference Presentations**

> Sumner, J. P., Westerberg, N. M., Stoddard A. K, Cramer, M. Thompson,
> R. B., Fierke, C. A., Kopelman, R., *Nanosized PEBBLE Sensors for the
> Detection of Cu2+/Cu+*,

-   *Pittcon*, March 2004.

> Sumner, J. P., Aylott, J., Fierke, C., Kopelman, R., *Pittcon,*
> *Luminescent Zinc Nanosensor for Real-Time Monitoring of Cellular
> Processes,*

-   March 2002.

> Sumner, J. P., Aylott, J., Kopelman, R., *Optochemical Zinc Nanosensor
> for Subcellular Chemical Imaging*, *Pittcon,* March 2001.
>
> Sumner, J. P., Xu, H., Aylott, J., Kopelman, R., *Subcellular Chemical
> Imaging for Zinc with an Optochemical Nanosensor,*

-   *FACSS,* October 2001.

> Sumner, J. P., DeVore, T., *JMU Spring Chemistry Symposium*, May 1999.
>
> Sumner, J. P., DeVore, T., *Southeast Regional ACS Meeting*, November
> 1998.
>
> Sumner, J. P., DeVore, T., *JMU Spring Chemistry Symposium*, May 1998.
>
> Sumner, J. P., DeVore, T., *National Conference on Undergraduate
> Research,* April 1998.
>
> Sumner, J. P., DeVore, T., *Southeast Regional ACS Meeting*, October
> 1997.