**Module 7: Preparation for Testing - Supplies and Kits**

+----------------+-----------------------------------------------------+
| **Purpose**    | To allow participants to become familiar and        |
|                | comfortable with the different supplies and         |
|                | materials required for HIV rapid testing.           |
+----------------+-----------------------------------------------------+
| **P            | None                                                |
| re-requisite** |                                                     |
|                |                                                     |
| **Modules**    |                                                     |
+----------------+-----------------------------------------------------+
| **Module       | 50 minutes                                          |
| Time**         |                                                     |
+----------------+-----------------------------------------------------+
| **Learning     | At the end of this module, participants will be     |
| Objectives**   | able to:                                            |
|                |                                                     |
|                | -   List and identify all the supplies required for |
|                |     HIV rapid testing                               |
|                |                                                     |
|                | -   List and identify all the components of test    |
|                |     kits for HIV rapid testing                      |
+----------------+-----------------------------------------------------+

**Module Overview**

+-----+-------+---------------+-----------------+----------------------+
| **  | **T   | **Activity/\  | **Content**     | **Resources\         |
| Ste | ime** | Method**      |                 | Needed**             |
| p** |       |               |                 |                      |
+-----+-------+---------------+-----------------+----------------------+
| **  | 2 min | >             | Module          | Slides 1-2           |
| 1** |       |  Presentation | introduction    |                      |
+-----+-------+---------------+-----------------+----------------------+
| **  | 15    | > Memory game | Supplies and    | Slide 3; materials & |
| 2** | min   |               | materials       | supplies             |
+-----+-------+---------------+-----------------+----------------------+
| **  | 10    | >             | Identifying     | Slides 4-18;         |
| 3** | min   |  Presentation | supplies &      | materials & supplies |
|     |       |               | materials       |                      |
+-----+-------+---------------+-----------------+----------------------+
| **  | 8 min | >             | Examining test  | Slide 19; test kits  |
| 4** |       | Presentation; | kits            |                      |
|     |       | >             |                 |                      |
|     |       | > Hands-on    |                 |                      |
|     |       | > activity    |                 |                      |
+-----+-------+---------------+-----------------+----------------------+
| **  | 10    | > Activity    | Organizing work | Slide 20; materials, |
| 5** | min   |               | area            | supplies, & test     |
|     |       |               |                 | kits                 |
+-----+-------+---------------+-----------------+----------------------+
| **  | 5 min | > Q&A         | Summary         | Slide 21             |
| 5** |       |               |                 |                      |
+-----+-------+---------------+-----------------+----------------------+

**Material/Equipment Checklists**

-   PowerPoint slides or transparencies

-   Overhead projector or computer w/LCD projector

-   Rapid testing materials, supplies, and kits laid out on a table --
    have enough sets for display and passing around

-   Handout: [Checklist of Supplies and Materials]{.underline}

**Teaching Guide**

+-----------------+-----------------------------------------------------+
| **Slide         | **Teaching Points**                                 |
| Number**        |                                                     |
+=================+=====================================================+
| **1**           | **[Module 7: Preparation for Testing -- Supplies    |
|                 | and Kits]{.underline}**                             |
|                 |                                                     |
|                 | DISPLAY this slide before you begin the module.     |
|                 | Make sure participants are aware of the transition  |
|                 | into a new module.                                  |
+-----------------+-----------------------------------------------------+
| **2**           | **[Learning Objectives]{.underline}**               |
|                 |                                                     |
|                 | STATE the objectives on the slide.                  |
+-----------------+-----------------------------------------------------+
| **3**           | **[Memory Game: Supplies and                        |
|                 | Materials]{.underline}**                            |
| **Game**        |                                                     |
|                 | DISPLAY all the materials and supplies in an        |
| **15 Minutes**  | exhibit area (e.g., at the back of the classroom).  |
|                 |                                                     |
|                 | INTRODUCE each item to the participants (about 5    |
|                 | min):                                               |
|                 |                                                     |
|                 | -   What it is                                      |
|                 |                                                     |
|                 | -   What it is used for                             |
|                 |                                                     |
|                 | CONDUCT the game according to instructions on the   |
|                 | slide.                                              |
|                 |                                                     |
|                 | DEBRIEF the game.                                   |
|                 |                                                     |
|                 | > ASK, "What materials and supplies are required    |
|                 | > for rapid testing?"                               |
|                 | >                                                   |
|                 | > WRITE participants' responses on a flipchart.     |
+-----------------+-----------------------------------------------------+
| ![](m           | When teaching participants [without a laboratory or |
| edia/image1.gif | medical background]{.underline}, allow more time    |
| ){width="0.4479 | for the game. But keep in mind that keeping the     |
| 166666666667in" | time compressed makes the game more challenging and |
| height="0.54166 | fun.                                                |
| 66666666666in"} |                                                     |
| ***TIPS***      |                                                     |
+-----------------+-----------------------------------------------------+
| **4-6**         | **[Supplies and Materials Checklist]{.underline}**  |
|                 |                                                     |
|                 | USE the checklist to see if all items are covered   |
|                 | by the participants.                                |
+-----------------+-----------------------------------------------------+
| ![](m           | DISPLAY each item.                                  |
| edia/image1.gif |                                                     |
| ){width="0.4479 | -   ASK, "What is this item used for?"              |
| 166666666667in" |                                                     |
| height="0.54166 | -   DISCUSS the answer.                             |
| 66666666666in"} |                                                     |
| ***TIPS***      | ALLOW participants to touch each item so they       |
|                 | become familiar with the different supplies         |
| **7-18**        |                                                     |
|                 | EMPHASIZE that these are examples of the types of   |
|                 | supplies that may be used in testing sites. That    |
|                 | is, brand names may differ from site to site.       |
|                 |                                                     |
|                 | NOTE: Key points are listed below.                  |
+-----------------+-----------------------------------------------------+
| **7**           | **[Gloves]{.underline}**                            |
|                 |                                                     |
|                 | -   Gloves are used for safety reasons, to protect  |
|                 |     both you and the patient or client.             |
|                 |                                                     |
|                 | -   It is important that the proper size gloves are |
|                 |     used. Wearing gloves that are too large may     |
|                 |     pose a safety hazard. and becomes cumbersome to |
|                 |     work with. Also, long nails may puncture the    |
|                 |     glove making them ineffective.                  |
|                 |                                                     |
|                 | -   Gloves must be changed between patients, and    |
|                 |     disposed of in a container labeled as           |
|                 |     bio-hazardous waste.                            |
|                 |                                                     |
|                 | -   Never use gloves that have been previously used |
|                 |     or are torn.                                    |
|                 |                                                     |
|                 | SPECIAL NOTE:                                       |
|                 |                                                     |
|                 | -   Consider latex allergies when selecting the     |
|                 |     type of glove to use                            |
+-----------------+-----------------------------------------------------+
| **8**           | **[Alcohol Swabs]{.underline}**                     |
|                 |                                                     |
|                 | -   Alcohol is used to cleanse the finger before    |
|                 |     pricking the client's finger.                   |
|                 |                                                     |
|                 | -   Alternatively, use a bottle of rubbing alcohol  |
|                 |     and cotton wool                                 |
|                 |                                                     |
|                 | SHOW rubbing alcohol and cotton wool if they are to |
|                 | be used in place of alcohol swabs.                  |
+-----------------+-----------------------------------------------------+
| **9**           | **[Cotton Gauze or Cotton Balls]{.underline}**      |
|                 |                                                     |
|                 | -   Cotton balls are used to wipe away the first    |
|                 |     drop of blood, and to stop bleeding after       |
|                 |     specimen is collected.                          |
|                 |                                                     |
|                 | -   They are single use                             |
|                 |                                                     |
|                 | -   Contaminated and should be disposed of with     |
|                 |     other hazardous waste.                          |
+-----------------+-----------------------------------------------------+
| **10**          | **[Sterile Lancets]{.underline}**                   |
|                 |                                                     |
|                 | -   There are a variety of lancets available for    |
|                 |     use. Some are easier to use than others.        |
|                 |                                                     |
|                 | -   One difference in the types of lancets is the   |
|                 |     depth of the puncture made by the retractable   |
|                 |     blade.                                          |
|                 |                                                     |
|                 | PASS AROUND the lancet that will be used            |
|                 | in-country.                                         |
+-----------------+-----------------------------------------------------+
| **11**          | **[Pipette]{.underline}**                           |
|                 |                                                     |
|                 | -   Pipettes are used to collect a specified volume |
|                 |     of blood specimen from the fingertip.           |
|                 |                                                     |
|                 | -   There are 2 types of pipettes commonly used.    |
|                 |                                                     |
|                 |     -   The transfer pipette is a disposable        |
|                 |         plastic item that is used only once. Be     |
|                 |         sure to dispose of this along with other    |
|                 |         contaminated waste.                         |
|                 |                                                     |
|                 |     -   The automatic pipette is used to collect a  |
|                 |         specified volume of blood and is most often |
|                 |         used in laboratories. A disposable tip is   |
|                 |         attached to the end of the pipette for      |
|                 |         collecting the blood. After use, the tip is |
|                 |         ejected or removed and is disposed of along |
|                 |         with other contaminated waste.              |
|                 |                                                     |
|                 |     -   The loop is another tool used to collect a  |
|                 |         specified volume of blood. This is used     |
|                 |         with some kits.                             |
|                 |                                                     |
|                 | PASS AROUND whichever pipette will be used          |
|                 | in-country                                          |
+-----------------+-----------------------------------------------------+
| **12**          | **[Timer]{.underline}**                             |
|                 |                                                     |
|                 | -   Shown here are two types of timer that can be   |
|                 |     used for waiting the specified time to elapse   |
|                 |     before test results are read.                   |
|                 |                                                     |
|                 | -   You may also use a watch, or clock.             |
+-----------------+-----------------------------------------------------+
| **13**          | **[Standard Operating Procedures and                |
|                 | Forms]{.underline}**                                |
|                 |                                                     |
|                 | -   Each site will also need to follow standard     |
|                 |     operating procedures, and use standard forms    |
|                 |     for recording test results.                     |
+-----------------+-----------------------------------------------------+
| **14**          | **[Labeling Pens and Writing Pens]{.underline}**    |
|                 |                                                     |
|                 | -   A permanent marker as seen on the left is best  |
|                 |     used for labeling test devices.                 |
|                 |                                                     |
|                 | -   Ball point pens are used to fill in forms.      |
|                 |                                                     |
|                 | -   Never use pencils, especially for recording     |
|                 |     client results -- results can be erased and     |
|                 |     changed....                                     |
+-----------------+-----------------------------------------------------+
| **15**          | **[Sharps Disposal Bins / Disinfectant              |
|                 | Jar]{.underline}**                                  |
|                 |                                                     |
|                 | POINT OUT that there are many different types of    |
|                 | disposal bins.                                      |
|                 |                                                     |
|                 | SHOW an example of a jar with disfectant and lid,   |
|                 | which can be used in the absence of a formal        |
|                 | disposal bin.                                       |
|                 |                                                     |
|                 | REMIND participants that all sharps containers must |
|                 | be labeled as bio-hazard waste. Stickers with a     |
|                 | symbol indicating biohazard waste may be available  |
|                 | in your area.                                       |
|                 |                                                     |
|                 | EMPHASIZE that regardless of the brand or type of   |
|                 | container, it should be one in which your hand      |
|                 | cannot reach inside.                                |
|                 |                                                     |
|                 | ASK participants to recall what bio-hazard mean.    |
+-----------------+-----------------------------------------------------+
| **16**          | **[Proper Disposal of Contaminated                  |
|                 | Waste]{.underline}**                                |
|                 |                                                     |
|                 | -   Image on the left -- Non-Sharps contaminated    |
|                 |     waste can be disposed of in a container that    |
|                 |     does not have a closable lid.                   |
|                 |                                                     |
|                 | -   Image on the right -- All sharps must be stored |
|                 |     in a container with a closable lid.             |
+-----------------+-----------------------------------------------------+
| **17**          | **[Waste Disposal]{.underline}**                    |
|                 |                                                     |
|                 | -   All containers for contaminated waste will      |
|                 |     likely not be red as seen in the previous       |
|                 |     images. Note the image on the left contains     |
|                 |     contaminated waste that has a closable lid.     |
|                 |     This floor model offers the option of stepping  |
|                 |     on the opener so that you hands will not have   |
|                 |     to touch the container.                         |
+-----------------+-----------------------------------------------------+
| **18**          | **[Household Bleach and Container]{.underline}**    |
|                 |                                                     |
|                 | POINT OUT:                                          |
|                 |                                                     |
|                 | -   The cup for measuring 1 part bleach and 9 parts |
|                 |     water                                           |
|                 |                                                     |
|                 | -   The pen used for labeling the spray bottle      |
|                 |                                                     |
|                 | -   The tape used for attaching to the bottle for   |
|                 |     labeling with the expiration date. Bleach       |
|                 |     solutions lose its disinfecting power after 7   |
|                 |     days.                                           |
+-----------------+-----------------------------------------------------+
| **19**          | **[Examine Test Kits]{.underline}**                 |
|                 |                                                     |
|                 | DISPLAY the kits that are used in-country, all the  |
|                 | components of each test kit laid out on a table.    |
|                 |                                                     |
|                 | SHOW the components of each kit.                    |
|                 |                                                     |
|                 | ALLOW participants to touch the kits so they become |
|                 | familiar with them.                                 |
+-----------------+-----------------------------------------------------+
| **20**          | **[Organize Your Work Area]{.underline}**           |
|                 |                                                     |
|                 | STATE that having an organized workspace is key to  |
|                 | producing quality results. It is important to:      |
|                 |                                                     |
|                 | -   Have necessary supplies placed within reach at  |
|                 |     the testing area before testing.                |
|                 |                                                     |
|                 | -   Keep working area neat, clean and organized.    |
|                 |                                                     |
|                 | EMPHASIZE that once the tester sits with the        |
|                 | client; it will make the client even more nervous   |
|                 | if the tester has to keep getting up to collect     |
|                 | more supplies.                                      |
+-----------------+-----------------------------------------------------+
| **Activity**    | ASK a participant volunteer to:                     |
|                 |                                                     |
| **10 Minutes**  | -   Organize a testing area with the supplies,      |
|                 |     materials, and test kits as the rest of the     |
|                 |     group observe.                                  |
|                 |                                                     |
|                 | Explain why the testing area is organized as such.  |
|                 |                                                     |
|                 | > All test materials on protective pad              |
|                 | >                                                   |
|                 | > Easy access to test materials (left-handed?)      |
|                 | >                                                   |
|                 | > No reaching across client (touching client with   |
|                 | > biohazard material)l or across biohazard          |
|                 | > container (avoid toppling over container)         |
|                 | >                                                   |
|                 | > Box lids open for easy access to materials        |
|                 |                                                     |
|                 | INVITE other participants to make comments or       |
|                 | suggestions.                                        |
|                 |                                                     |
|                 | DEBRIEF by discussing what makes a good testing     |
|                 | area.                                               |
+-----------------+-----------------------------------------------------+
| **21**          | **[Summary]{.underline}**                           |
|                 |                                                     |
|                 | ASK participants to answer the questions on the     |
|                 | slide.                                              |
|                 |                                                     |
|                 | ANSWER any questions participants may have.         |
+-----------------+-----------------------------------------------------+