# Module 4: Managing Supplies for Sputum Smear Microscopy

+-------------+--------------------------------------------------------+
| **Purpose** | To provide participants with knowledge and skills of   |
|             | ordering and stocking and maintaining materials for    |
|             | the proper functioning of a TB laboratory.             |
+-------------+--------------------------------------------------------+
| **Pre-      | None                                                   |
| requisite** |                                                        |
|             |                                                        |
| **Modules** |                                                        |
+-------------+--------------------------------------------------------+
| **Module    | 2 hours 35 minutes                                     |
| Time**      |                                                        |
+-------------+--------------------------------------------------------+
| **Learning  | At the end of this module, participants will be able   |
| O           | to                                                     |
| bjectives** |                                                        |
|             | -   List supplies required to perform smear microscopy |
|             |                                                        |
|             | -   Explain ordering of supplies                       |
|             |                                                        |
|             | -   Maintain proper records                            |
|             |                                                        |
|             | -   Explain use of stock book                          |
|             |                                                        |
|             | -   Calculate supplies required                        |
|             |                                                        |
|             | -   Inspect and verify supplies received               |
|             |                                                        |
|             | ```{=html}                                             |
|             | <!-- -->                                               |
|             | ```                                                    |
|             | -   Explain storage of supplies.                       |
+-------------+--------------------------------------------------------+

**Module Overview**

+----+------+------------+-----------------------+--------------------+
| ** | **Ti | **Activity | **Content**           | **Resources\       |
| St | me** | /\         |                       | Needed**           |
| ep |      | Method**   |                       |                    |
| ** |      |            |                       |                    |
+----+------+------------+-----------------------+--------------------+
| 1  | 15   | Pr         | Module Introduction   | Slides 1--3        |
|    | min  | esentation |                       |                    |
+----+------+------------+-----------------------+--------------------+
| 2  | 10   | Pr         | Managing Supplies     | Slides 4--8        |
|    | min  | esentation |                       |                    |
+----+------+------------+-----------------------+--------------------+
|    | 30   | > Pr       | Inventory records     | Slides 9--11       |
|    | min  | esentation |                       |                    |
+----+------+------------+-----------------------+--------------------+
| 3  | 1 hr | Group      | Calculation of        | Slides 12--26      |
|    | 15   | Activity   | supplies for a        | (**[o              |
|    | min  |            | microscopy center     | nly]{.underline}** |
|    |      |            | using a worksheet and | Laboratories that  |
|    |      |            | calculator            | place their own    |
|    |      |            |                       | orders for         |
|    |      |            |                       | prepared staining  |
|    |      |            |                       | reagents)          |
|    |      |            |                       |                    |
|    |      |            |                       | Slides 27--41      |
|    |      |            |                       | (**[o              |
|    |      |            |                       | nly]{.underline}** |
|    |      |            |                       | for Laboratories   |
|    |      |            |                       | that place their   |
|    |      |            |                       | own orders for     |
|    |      |            |                       | reagent components |
|    |      |            |                       | for making         |
|    |      |            |                       | staining reagents) |
|    |      |            |                       |                    |
|    |      |            |                       | Flipchart,         |
|    |      |            |                       | Worksheet          |
|    |      |            |                       |                    |
|    |      |            |                       | Hand outs,         |
|    |      |            |                       |                    |
|    |      |            |                       | Calculator         |
+----+------+------------+-----------------------+--------------------+
| 4  | 15   | Pr         | Placing, Receiving an | Slides 42--44      |
|    | min  | esentation | Storing Supplies      |                    |
+----+------+------------+-----------------------+--------------------+
| 5  | 10   | Pr         | Summary               | Slides 45          |
|    | min  | esentation |                       |                    |
|    |      | and        |                       |                    |
|    |      | Discussion |                       |                    |
+----+------+------------+-----------------------+--------------------+

## Material and Equipment Checklists

-   Power Point presentation or transparencies

-   Overhead projector and/or computer w/LCD projector

-   Flips chart or white board for individual calculations

-   One calculator for each participant and trainer

-   AFB Laboratory Performance and Stocks Form

-   Stock Book

-   Exercise 1: Calculation of quarterly supply requirements for a
    microscopy center (for a microscopy center that receives prepared
    staining reagents

-   Exercise 2: Calculation of quarterly supply requirements for a
    microscopy center (for a microscopy center that prepares its own
    straining reagents)

##### How to Present this Module

+-------------------------+---------------------+---------------------+
| Does your lab receive:  | Does your           |                     |
|                         | laboratory place    |                     |
|                         | its own orders?     |                     |
+-------------------------+---------------------+---------------------+
|                         | Yes                 | No                  |
+-------------------------+---------------------+---------------------+
| Prepared Staining       | Present Core        | Present Core        |
| Reagents?               | curriculum\* and    | curriculum\*        |
|                         |                     |                     |
|                         | Exercise 1          |                     |
+-------------------------+---------------------+---------------------+
| Individual Reagents for | Present Core        | Present Core        |
| Preparing Staining      | curriculum\* and    | curriculum\*        |
| Reagents?               | Exercise 2          |                     |
+-------------------------+---------------------+---------------------+

\*The Core curriculum consists of the text on supplies, the PowerPoint
presentation, the stock book form, and the AFB Laboratory Performance
and Stock form for recording workload and inventory. All laboratories
should complete the AFB Laboratory Performance and Stock form at the end
of each quarter. For laboratories that do not place their own orders,
the information recorded on the AFB Laboratory Performance and Stock is
reported to the level where order amounts are determined and disbursed.
For laboratories that place their own orders, the information recorded
on the AFB Laboratory Performance and Stock is used by that laboratory
to calculate the quantities of supply items they require, based on their
actual workload and inventory. Laboratories that place their own orders
for prepared staining reagents perform Exercise 1. Laboratories that
place their own orders for reagent components and use them to prepare
their own staining reagents perform Exercise 2.

**Teaching Guide**

+-------------------+--------------------------------------------------+
| **Slide Number**  | **Teaching Points**                              |
+===================+==================================================+
| 1.                | **[Module 4: Managing Supplies for Sputum Smear  |
|                   | Microscopy]{.underline}**                        |
|                   |                                                  |
|                   | DISPLAY this slide before you begin the module.  |
|                   | Make sure participants are aware of the          |
|                   | transition into a new module.                    |
+-------------------+--------------------------------------------------+
| 2.                | **[Learning Objectives: Supply                   |
|                   | Management]{.underline}**                        |
|                   |                                                  |
|                   | STATE the objectives on the slide                |
+-------------------+--------------------------------------------------+
| 3.  **Flipchart*  | **[Content Overview]{.underline}**               |
| *![](media/image1 |                                                  |
| .wmf){width="0.84 | (Suggested presentation technique)               |
| 23611111111111in" |                                                  |
|     height="0.842 | WRITE the content outline on a flipchart prior   |
| 3611111111111in"} | to training.                                     |
|                   |                                                  |
|                   | > REFER to it frequently to orient participants  |
|                   | > to where they are in the module.               |
+-------------------+--------------------------------------------------+
| 4.                | **[Supply Management Means:]{.underline}**       |
|                   |                                                  |
|                   | DEFINE Supply Management as indicated on the     |
|                   | slide.                                           |
+-------------------+--------------------------------------------------+
| 5.                | **[NTP Specific Customization]{.underline}**     |
|                   |                                                  |
| > ![              | Customize and Explain your countries specific    |
| ](media/image2.wm | NTP guidelines for the preparation of stains and |
| f){width="0.75in" | distribution. Describe the stain concentration,  |
| > height="0.673   | device (loop or stick) used for smearing, use of |
| 6111111111112in"} | frosted or unfrosted slides, and method of waste |
|                   | decontamination                                  |
+-------------------+--------------------------------------------------+
| 6.                | ### Ordering and Distribution of Supplies        |
|                   |                                                  |
|                   | EXPLAIN ordering and distributing supplies       |
|                   | involves knowing the answers to the questions.   |
|                   | By the end of this module, each participant      |
|                   | should be able to answer these questions in      |
|                   | order to properly maintain supplies needed for   |
|                   | AFB Direct Smear Microscopy.                     |
+-------------------+--------------------------------------------------+
| 7.                | **[Basic Supplies Required for AFB Smear         |
|                   | Microscopy]{.underline}**                        |
|                   |                                                  |
|                   | DISCUSS the list of supplies needed to perform   |
|                   | AFB Direct Smear Microscopy.                     |
|                   |                                                  |
|                   | EXPLAIN the purpose of each item on the list.    |
|                   |                                                  |
|                   | SETUP a workstation with all supplies labeled.   |
|                   |                                                  |
|                   | ASK participants to visit the table with         |
|                   | supplies to become familiar with them before the |
|                   | laboratory practical session.                    |
+-------------------+--------------------------------------------------+
| 8.                | ### Supply Management Involves...                |
|                   |                                                  |
|                   | -   Performing a "stock count"                   |
|                   |                                                  |
|                   | -   Maintaining proper inventory records         |
|                   |                                                  |
|                   | -   Determining how much to order                |
|                   |                                                  |
|                   | -   Placing orders properly                      |
|                   |                                                  |
|                   | -   Inspecting and verifying supplies received   |
|                   |                                                  |
|                   | -   Ensuring proper storage of stock             |
+-------------------+--------------------------------------------------+
| 9.                | ### Stock Book                                   |
|                   |                                                  |
|                   | EXPLAIN that the stock book contains a list of   |
|                   | all items in the lab                             |
|                   |                                                  |
|                   | -   Update it when orders are placed and         |
|                   |     received                                     |
|                   |                                                  |
|                   | -   Serves as a reference to track orders that   |
|                   |     have been placed and not received            |
|                   |                                                  |
|                   | -   Information recorded about when orders are   |
|                   |     placed and arrive may help to adjust the     |
|                   |     reserve quantities of supplies               |
|                   |                                                  |
|                   | -   Provides information of stock in hand        |
|                   |                                                  |
|                   | EXPLAIN that an efficient stock management       |
|                   | depends upon accurate record keeping.            |
+-------------------+--------------------------------------------------+
| 10.               | ### Perform a Stock Count                        |
|                   |                                                  |
|                   | EXPLAIN the meaning of stock count and how it is |
|                   | different from the previous slide.               |
|                   |                                                  |
|                   | Physically counting the quantity of each item    |
|                   | present in the stock                             |
|                   |                                                  |
|                   | Recommended at the end of each quarter or follow |
|                   | country specific guidelines, if present.         |
|                   |                                                  |
|                   | A person must be designated to perform the stock |
|                   | count. Stock count must be recorded with a proof |
|                   | of signature and date.                           |
|                   |                                                  |
|                   | EMPHASIZE that all items must be accounted for.  |
|                   | Everything that is ordered and received must be  |
|                   | recorded.                                        |
+-------------------+--------------------------------------------------+
| 11.               | ### AFB Laboratory Performance and Stock Form    |
|                   |                                                  |
|                   | EXPLAIN that this form is used at end of each    |
|                   | quarter to report work (# of smear examined) and |
|                   | stock on hand                                    |
|                   |                                                  |
|                   | Work performed is captured by reviewing the TB   |
|                   | register and counting the number of smears       |
|                   | resulted as positive, negative, or scanty (1--9  |
|                   | AFB/100 fields) from both TB suspect patients    |
|                   | and follow-up patients                           |
|                   |                                                  |
|                   | Stock on hand is determined by performing a      |
|                   | physical inventory                               |
+-------------------+--------------------------------------------------+
| 12.               | ### Methods for Calculating Supplies             |
|                   |                                                  |
|                   | Describe the 10% positive case rate method.      |
|                   |                                                  |
|                   | -   The factor of 33 smears per positive case    |
|                   |     detected is based on the assumption that the |
|                   |     average positively rate of suspects in the   |
|                   |     labs is 10% and that 3 smears are examined   |
|                   |     per suspect and 3 more for follow-up of      |
|                   |     smear-positive cases                         |
|                   |                                                  |
|                   | -   Correct this factor if average positively    |
|                   |     rate of suspects in the labs deviates        |
|                   |     considerably from 10%, or if the number of   |
|                   |     sputa examined per suspect / case has been   |
|                   |     specified differently in national            |
|                   |     guidelines.                                  |
|                   |                                                  |
|                   | Read the second method from the slide            |
+-------------------+--------------------------------------------------+
| ![](media/image2  | Show slides 13 -- 26 only to laboratorians that  |
| .wmf){width="0.74 | place their own orders for prepared staining     |
| 72222222222222in" | reagents                                         |
| height="0.670     |                                                  |
| 8333333333333in"} |                                                  |
+-------------------+--------------------------------------------------+
| 13.               | Calculated Quarterly Requirements for Peripheral |
|                   | Microscopy Center                                |
|                   |                                                  |
|                   | > REFER participants to the full-page worksheet  |
|                   | > in the participant's manual.                   |
|                   | >                                                |
|                   | > DESCRIBE the form and mention that each of the |
|                   | > columns will be described in detail in the     |
|                   | > next slides                                    |
+-------------------+--------------------------------------------------+
| 14.               | Total slides examined previous quarter (A)       |
|                   |                                                  |
|                   | > STATE the message on the slide:                |
|                   | >                                                |
|                   | > The value (A) equals the number of sputum      |
|                   | > examinations performed during the previous     |
|                   | > quarter                                        |
|                   | >                                                |
|                   | > Ask if it is clear.                            |
+-------------------+--------------------------------------------------+
| 15.               | #### Examples of Items Required                  |
|                   |                                                  |
|                   | > EXPLAIN that this is the first column on the   |
|                   | > worksheet                                      |
|                   | >                                                |
|                   | > GIVE examples of items for AFB smear           |
|                   | > microscopy                                     |
+-------------------+--------------------------------------------------+
| 16.               | ### The                                          |
|                   | quantity required for one sputum examination is? |
|                   |                                                  |
|                   | EXPLAIN that this is the second column on the    |
|                   | worksheet                                        |
|                   |                                                  |
|                   | Ask the participants how much of each item they  |
|                   | think is required for one sputum examination     |
|                   |                                                  |
|                   | You can ask for a show of hands or use a flip    |
|                   | chart to record audience responses before moving |
|                   | to next slide                                    |
+-------------------+--------------------------------------------------+
| 17.               | ### The                                          |
|                   |  quantity required for one sputum examination is |
|                   |                                                  |
|                   | STATE the answers and explain that they          |
|                   | represent typical amount and will be used in the |
|                   | following calculations                           |
+-------------------+--------------------------------------------------+
| 18.               | **[Calculation for One Quarter of Supply of an   |
|                   | Item (C)]{.underline}**                          |
|                   |                                                  |
|                   | EXPLAIN the column (C) and the calculation.      |
+-------------------+--------------------------------------------------+
| 19.               | Example to Calculate One Quarter of Supply of    |
|                   | Carbol fuchsin (C)                               |
|                   |                                                  |
|                   | EXPLAIN the example on the slide and clear any   |
|                   | doubts arising                                   |
+-------------------+--------------------------------------------------+
| 20.               | ### Reserve Quantity (D)                         |
|                   |                                                  |
|                   | EXPLAIN the column (D) and importance of reserve |
|                   | quantity for uninterrupted work.                 |
+-------------------+--------------------------------------------------+
| 21.               | Stock on Hand (E)                                |
|                   |                                                  |
|                   | EXPLAIN the column (E) and how the stock on hand |
|                   | is counted                                       |
+-------------------+--------------------------------------------------+
| 22.               | Calculated request (F)                           |
|                   |                                                  |
|                   | EXPLAIN the column (F) and the calculation       |
+-------------------+--------------------------------------------------+
| 23.               | ### Quantities requested/ issued                 |
|                   |                                                  |
|                   | EXPLAIN the rounding of the calculated quantity  |
|                   | to the next whole number of unit of issue        |
|                   |                                                  |
|                   | EXPLAIN the two examples                         |
|                   |                                                  |
|                   | -   Example 1, if the calculated quantity of     |
|                   |     carbol fuchsin is 1824 ml, round up to 2     |
|                   |     liters                                       |
|                   |                                                  |
|                   | -   Example 2, if the calculated quantity of     |
|                   |     slides is 700, round up to 720 since the     |
|                   |     unit of issue is 72 slides per box (i.e.     |
|                   |     request 10 boxes)                            |
+-------------------+--------------------------------------------------+
| 24.               | Exercise #1: Calculate Quarterly Requirements    |
|                   | for your Microscopy Center                       |
|                   |                                                  |
|                   | PROVIDE instructions for completing exercise     |
|                   |                                                  |
|                   | -   Refer participants to handout labeled        |
|                   |     exercise #1                                  |
|                   |                                                  |
|                   | -   Instruct participants to work individually   |
|                   |                                                  |
|                   | -   Instruct how to use the calculators provided |
|                   |                                                  |
|                   | -   Instruct participants on amount of time      |
|                   |     allowed for exercise (30 minutes)            |
+-------------------+--------------------------------------------------+
| >                 | *Beforehand, prepare a flipchart showing the     |
|  ![](media/image3 | following data but do not display until this     |
| .gif){width="0.44 | slide*                                           |
| 79166666666667in" |                                                  |
| > height="0.541   | -   Total slides examined in previous quarter    |
| 6666666666666in"} |     (A)= 463                                     |
| >                 |                                                  |
| > **TIPS**        | -   Quantity needed per smear (B) for each item  |
|                   |                                                  |
|                   |     -   Sputum containers 1 piece                |
|                   |                                                  |
|                   |     -   Slides 1 piece                           |
|                   |                                                  |
|                   |     -   Carbol fuchsin 3 ml                      |
|                   |                                                  |
|                   |     -   Destaining reagent 5 ml                  |
|                   |                                                  |
|                   |     -   Methylene blue 3 ml                      |
|                   |                                                  |
|                   |     -   Immersion oil 0.05 ml                    |
|                   |                                                  |
|                   |     -   Burning spirit 1 ml                      |
+-------------------+--------------------------------------------------+
| 25.               | Exercise #1: Calculate Quarterly Requirements    |
|                   | for your Microscopy Center                       |
|                   |                                                  |
|                   | Show the slide until the end of the exercise     |
|                   |                                                  |
|                   | -   Use worksheet labeled exercise #1            |
|                   |                                                  |
|                   | -   Work individually                            |
|                   |                                                  |
|                   | -   Use sample data provided on flipchart        |
|                   |                                                  |
|                   | -   Use calculators provided                     |
|                   |                                                  |
|                   | -   Time allowed: 30 minutes                     |
+-------------------+--------------------------------------------------+
| 26.               | Exercise #1 Answer\                              |
|                   | Calculated Quarterly Requirements for Your       |
|                   | Microscopy Center                                |
|                   |                                                  |
|                   | SHOW the completed worksheet                     |
|                   |                                                  |
|                   | REVIEW and discuss the calculation process       |
|                   |                                                  |
|                   | Use flip chart to record discussion points       |
+-------------------+--------------------------------------------------+
| > ![](m           | Show slides 27--41 only to laboratorians that    |
| edia/image2.wmf){ | place their own orders for reagent components    |
| width="0.73125in" | and use them to prepare their own staining       |
| > height="0.656   | reagents                                         |
| 9444444444444in"} |                                                  |
+-------------------+--------------------------------------------------+
| 27.               | Calculated Quarterly Requirements for Peripheral |
|                   | Microscopy Center                                |
|                   |                                                  |
|                   | > REFER participants to the full-page worksheet  |
|                   | > in the participant's manual.                   |
|                   | >                                                |
|                   | > DESCRIBE the form and mention that each of the |
|                   | > columns will be described in detail in the     |
|                   | > next slides                                    |
+-------------------+--------------------------------------------------+
| 28.               | Total slides examined previous quarter (A)       |
|                   |                                                  |
|                   | > EXPLAIN the term (A) and STATE the message on  |
|                   | > the slide                                      |
+-------------------+--------------------------------------------------+
| 29.               | #### Examples of Items Required                  |
|                   |                                                  |
|                   | > EXPLAIN that this is the first column (Items)  |
|                   | > on the worksheet and the unit of use           |
+-------------------+--------------------------------------------------+
| 30.               | ### The                                          |
|                   | quantity required for one sputum examination is? |
|                   |                                                  |
|                   | EXPLAIN that this is the second column (B) on    |
|                   | the worksheet                                    |
|                   |                                                  |
|                   | Ask the participants how much of each item they  |
|                   | think is required for one sputum examination     |
|                   |                                                  |
|                   | Can ask for a show of hands or use a flip chart  |
|                   | to record audience responses                     |
+-------------------+--------------------------------------------------+
| 31.               | ### The qua                                      |
|                   | ntity required for one sputum (B) examination is |
|                   |                                                  |
|                   | ##### DO NO                                      |
|                   | T include this slide in the participants handout |
|                   |                                                  |
|                   | > STATE message on slide                         |
|                   | >                                                |
|                   | > Explain that liquid quantities are expressed   |
|                   | > as liters, rather than milliliters for         |
|                   | > uniformity of units during calculations        |
|                   |                                                  |
|                   | State the answers and explain that they          |
|                   | represent typical amount and will be used in the |
|                   | following calculations                           |
+-------------------+--------------------------------------------------+
| 32.               | **[The reagent quantity per liter (C)            |
|                   | is]{.underline}**                                |
|                   |                                                  |
|                   | STATE message on slide                           |
+-------------------+--------------------------------------------------+
| 33.               | **[Calculation for One Quarter of Supply of an   |
|                   | Item (D)]{.underline}**                          |
|                   |                                                  |
|                   | STATE message on slide and EXPLAIN the           |
|                   | calculation                                      |
+-------------------+--------------------------------------------------+
| 34.               | Example to Calculate One Quarter of Supply of    |
|                   | Basic Fuchsin (D)                                |
|                   |                                                  |
|                   | EXPLAIN and walk through the calculation with    |
|                   | the participants                                 |
+-------------------+--------------------------------------------------+
| 35.               | ### Reserve Quantity (E)                         |
|                   |                                                  |
|                   | EXPLAIN the message on slide                     |
+-------------------+--------------------------------------------------+
| 36.               | Stock on Hand (F)                                |
|                   |                                                  |
|                   | STATE the message on slide                       |
+-------------------+--------------------------------------------------+
| 37.               | Calculated request (G)                           |
|                   |                                                  |
|                   | STATE message on slide                           |
+-------------------+--------------------------------------------------+
| 38.               | ### Quantities requested/ issued                 |
|                   |                                                  |
|                   | STATE message on slide                           |
|                   |                                                  |
|                   | EXPLAIN the two examples                         |
+-------------------+--------------------------------------------------+
| 39.               | Exercise #2: Calculate Quarterly Requirements    |
|                   | for your Microscopy Center                       |
|                   |                                                  |
|                   | PROVIDE instructions for completing exercise     |
|                   |                                                  |
|                   | REFER participants to handout labeled exercise   |
|                   | #2                                               |
|                   |                                                  |
|                   | STATE message on slide                           |
|                   |                                                  |
|                   | -   Instruct how to use the calculators provided |
|                   |                                                  |
|                   | -   Instruct participants on amount of time      |
|                   |     allowed for exercise (30 minutes)            |
+-------------------+--------------------------------------------------+
| 40.               | Exercise #2: Calculate Quarterly Requirements    |
|                   | for your Microscopy Center                       |
|                   |                                                  |
|                   | Show the slide until the end of the exercise     |
+-------------------+--------------------------------------------------+
| 41.               | Exercise #2 Answer\                              |
|                   | Calculated Quarterly Requirements for Your       |
|                   | Microscopy Center                                |
|                   |                                                  |
|                   | Show the completed worksheet                     |
|                   |                                                  |
|                   | Review and discuss the calculation process       |
|                   |                                                  |
|                   | Use flip chart to record discussion points       |
+-------------------+--------------------------------------------------+
| 42.               | Place Orders Properly                            |
|                   |                                                  |
| ![](media/image2  | *This slide must be customized with              |
| .wmf){width="0.63 | site-specific information obtained beforehand*   |
| 54166666666666in" |                                                  |
| height="0.570     | EXPLAIN from the slide                           |
| 8333333333333in"} |                                                  |
+-------------------+--------------------------------------------------+
| 43.               | Inspect & Verify the Delivery of Orders Upon     |
|                   | receipt:                                         |
|                   |                                                  |
|                   | EXPLAIN what should you do when you receive the  |
|                   | requested supplies                               |
+-------------------+--------------------------------------------------+
| 44.               | Ensure Proper Storage of Inventory               |
|                   |                                                  |
|                   | STATE the points on the slide.                   |
+-------------------+--------------------------------------------------+
| 45.               | # [Summary]{.underline}                          |
|                   |                                                  |
|                   | ASK the participants to answer the questions on  |
|                   | the slide.                                       |
|                   |                                                  |
|                   | ANSWER any questions the participants may have.  |
+-------------------+--------------------------------------------------+

###### MODULE 4

###### ANSWERS: EXERCISE 1

+--------------+-----+-------+---+---+----+-------+---------+----------+
| **Quarterly  |     |       |   |   |    |       |         |          |
| Supply       |     |       |   |   |    |       |         |          |
| Requirements |     |       |   |   |    |       |         |          |
| for a        |     |       |   |   |    |       |         |          |
| Microscopy   |     |       |   |   |    |       |         |          |
| Centre**     |     |       |   |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
|              |     |       |   |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Region:      |     |       |   | S |    |       |         |          |
|              |     |       |   | u |    |       |         |          |
|              |     |       |   | p |    |       |         |          |
|              |     |       |   | p |    |       |         |          |
|              |     |       |   | l |    |       |         |          |
|              |     |       |   | y |    |       |         |          |
|              |     |       |   | q |    |       |         |          |
|              |     |       |   | u |    |       |         |          |
|              |     |       |   | a |    |       |         |          |
|              |     |       |   | r |    |       |         |          |
|              |     |       |   | t |    |       |         |          |
|              |     |       |   | e |    |       |         |          |
|              |     |       |   | r |    |       |         |          |
|              |     |       |   | : |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| District:    |     |       |   | Y |    |       |         |          |
|              |     |       |   | e |    |       |         |          |
|              |     |       |   | a |    |       |         |          |
|              |     |       |   | r |    |       |         |          |
|              |     |       |   | : |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Centre:      |     |       |   |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Total smears |     |       |   |   |    |       |         |          |
| examined in  |     |       |   |   |    |       |         |          |
| previous     |     |       |   |   |    |       |         |          |
| quarter      |     |       |   |   |    |       |         |          |
| (**A**)= 500 |     |       |   |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Items        | Qu  | Calcu | R |   | S  | Calcu | Actual  | Order    |
|              | ant | lated | e |   | to | lated | request | unit\*\* |
|              | ity | re    | s |   | ck | re    | (rou    |          |
|              | nee | quire | e |   | on | quest | nded\*) |          |
|              | ded | ments | r |   | ha |       |         |          |
|              | per | for   | v |   | nd | (*    |         |          |
|              | sm  | one   | e |   |    | *F**) |         |          |
|              | ear | qu    | q |   | (  | =     |         |          |
|              |     | arter | u |   | ** | **    |         |          |
|              | (   |       | a |   | E* | C**+* |         |          |
|              | **B | (*    | n |   | *) | *D**- |         |          |
|              | **) | *C**) | t |   |    | **E** |         |          |
|              |     | =     | i |   |    |       |         |          |
|              |     | *     | t |   |    |       |         |          |
|              |     | *A**x | y |   |    |       |         |          |
|              |     | **B** | f |   |    |       |         |          |
|              |     |       | o |   |    |       |         |          |
|              |     |       | r |   |    |       |         |          |
|              |     |       | o |   |    |       |         |          |
|              |     |       | n |   |    |       |         |          |
|              |     |       | e |   |    |       |         |          |
|              |     |       | q |   |    |       |         |          |
|              |     |       | u |   |    |       |         |          |
|              |     |       | a |   |    |       |         |          |
|              |     |       | r |   |    |       |         |          |
|              |     |       | t |   |    |       |         |          |
|              |     |       | e |   |    |       |         |          |
|              |     |       | r |   |    |       |         |          |
|              |     |       | ( |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | D |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | ) |   |    |       |         |          |
|              |     |       | = |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | C |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
|              |     |       | * |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Sputum       | 1   | 500   | 5 |   | 58 | 942   | 1       | Bag      |
| containers   |     |       | 0 |   |    |       |         | (1000    |
| (pieces)     |     |       | 0 |   |    |       |         | count)   |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Slides       | 1   | 500   | 5 |   | 50 | 950   | 14      | Box (72  |
| (pieces)     |     |       | 0 |   |    |       |         | slides)  |
|              |     |       | 0 |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Carbol       | 3.0 | 1500  | 1 |   | 2  | 2800  | 3       | Bottle   |
| fuchsin (mL) |     |       | 5 |   | 00 |       |         | (1       |
|              |     |       | 0 |   |    |       |         | liter)   |
|              |     |       | 0 |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Destaining   | 5.0 | 2500  | 2 |   | 1  | 4850  | 5       | Bottle   |
| reagent (mL) |     |       | 5 |   | 50 |       |         | (1       |
|              |     |       | 0 |   |    |       |         | liter)   |
|              |     |       | 0 |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Methylene    | 3.0 | 1500  | 1 |   | 35 | -500  | 0       | Bottle   |
| blue (mL)    |     |       | 5 |   | 00 |       |         | (1       |
|              |     |       | 0 |   |    |       |         | liter)   |
|              |     |       | 0 |   |    |       |         |          |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Immersion    | 0   | 25    | 2 |   | 10 | 40    | 2       | Bottle   |
| oil (mL)     | .05 |       | 5 |   |    |       |         | (50 mL)  |
+--------------+-----+-------+---+---+----+-------+---------+----------+
| Burning      | 1   | 500   | 5 |   | 1  | 900   | 1       | Bottle   |
| spirit (mL)  |     |       | 0 |   | 00 |       |         | (1       |
|              |     |       | 0 |   |    |       |         | liter)   |
+--------------+-----+-------+---+---+----+-------+---------+----------+

\*Round up to the next indent unit

\*\* Change to your country specific units

**MODULE 4**

**ANSWERS: EXERCISE 2**

+---------+-----+----+-------+-----+-----+-------+-------+----------+
| **Qu    |     |    |       |     |     |       |       |          |
| arterly |     |    |       |     |     |       |       |          |
| Supply  |     |    |       |     |     |       |       |          |
| Requi   |     |    |       |     |     |       |       |          |
| rements |     |    |       |     |     |       |       |          |
| for a   |     |    |       |     |     |       |       |          |
| Mic     |     |    |       |     |     |       |       |          |
| roscopy |     |    |       |     |     |       |       |          |
| C       |     |    |       |     |     |       |       |          |
| entre** |     |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
|         |     |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Region: |     |    |       | Sup |     |       |       |          |
|         |     |    |       | ply |     |       |       |          |
|         |     |    |       | qu  |     |       |       |          |
|         |     |    |       | art |     |       |       |          |
|         |     |    |       | er: |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| D       |     |    |       | Ye  |     |       |       |          |
| istrict |     |    |       | ar: |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Centre  |     |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Total   |     |    |       |     |     |       |       |          |
| smears  |     |    |       |     |     |       |       |          |
| e       |     |    |       |     |     |       |       |          |
| xamined |     |    |       |     |     |       |       |          |
| in      |     |    |       |     |     |       |       |          |
| p       |     |    |       |     |     |       |       |          |
| revious |     |    |       |     |     |       |       |          |
| quarter |     |    |       |     |     |       |       |          |
| (       |     |    |       |     |     |       |       |          |
| **A**)= |     |    |       |     |     |       |       |          |
| *       |     |    |       |     |     |       |       |          |
| *1200** |     |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Items   | Qu  | R  | Calcu | R   | St  | Calcu | A     | Order    |
|         | ant | ea | lated | ese | ock | lated | ctual | unit     |
|         | ity | ge | re    | rve | on  | re    | re    |          |
|         | nee | nt | quire | qu  | h   | quest | quest |          |
|         | ded | qu | ments | ant | and |       | (ro   |          |
|         | per | an | for   | ity |     | **(G) | unded |          |
|         | sm  | ti | one   | for | *   | =     | \*\*) |          |
|         | ear | ty | qu    | one | *(F | D+    |       |          |
|         |     | p  | arter | q   | )** | E-F** |       |          |
|         | (   | er |       | uar |     |       |       |          |
|         | **B | l  | *     | ter |     |       |       |          |
|         | **) | it | *(D)= |     |     |       |       |          |
|         |     | er | Ax    | **( |     |       |       |          |
|         |     |    | BxC** | E)= |     |       |       |          |
|         |     | (  |       | D** |     |       |       |          |
|         |     | ** |       |     |     |       |       |          |
|         |     | C* |       |     |     |       |       |          |
|         |     | *) |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Sputum  |     | N  | 1200  | 1   | 500 | 1900  | 2     | Bag      |
| con     |     | /A | pcs   | 200 | pcs | pcs   |       | (1000    |
| tainers |     |    |       | pcs |     |       |       | count)   |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Slides  | 1   | N  | 1200  | 1   | 432 | 1968  | 2     | Case     |
|         | pc  | /A | pcs   | 200 | pcs | pcs   |       | (1728    |
|         |     |    |       | pcs |     |       |       | sl       |
|         |     |    |       |     |     |       |       | ides)^±^ |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Basic   | 0.  | 3  | 10.8  | 1   | 0 g | 21.6  | 1     | Bottle   |
| fu      | 003 | g  | g     | 0.8 |     | g     |       | (25g)    |
| chsin\* | Lt  |    |       | g   |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Phenol  | 0.  | 50 | 180 g | 180 | 100 | 260 g | 3     | Bottle   |
|         | 003 | g  |       | g   | g   |       |       | (100 g)  |
|         | Lt  |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| S       | 0.  | 0  | 1.5   | 1.5 | 1   | 2 Lt  | 2     | Bottle   |
| ulfuric | 005 | .2 | Lt    | Lt  | Lt  |       |       | (1L)     |
| acid    | Lt  | 50 |       |     |     |       |       |          |
|         |     | Lt |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Me      | 0.  | 3  | 10.8  | 1   | 25  | -3.4  | 0     | Bottle   |
| thylene | 003 | g  | g     | 0.8 | g   | g     |       | (25g)    |
| blue    | Lt  |    |       | g   |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| De      | 0.  | 0  | 0.36  | 0   | 0.5 | 0.22  | 1     | Bottle   |
| natured | 003 | .1 | Lt    | .36 | Lt  | Lt    |       | (500 mL) |
| alcohol | Lt  | 00 |       | Lt  |     |       |       |          |
|         |     | Lt |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Burning | 0.  | N  | 1.2   | 1.2 | 0.5 | 1.9   | 1     | Canister |
| spirit  | 001 | /A | Lt    | Lt  | Lt  | Lt    |       | (5 Lt)   |
|         | Lt  |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+
| Im      | 0   | N  | 0.06  | 0   | .05 | 0.07  | 2     | Bottle   |
| mersion | .00 | /A | Lt    | .06 | Lt  | Lt    |       | (50 mL)  |
| oil     | 005 |    |       | Lt  |     |       |       |          |
|         | Lt  |    |       |     |     |       |       |          |
+---------+-----+----+-------+-----+-----+-------+-------+----------+

\* Consider carbol fuchsin concentration in use

\*\*Round up to the next indent unit

\*\*\* Change to your country specific units

^±^ Case = 24 boxes X 72 slides