Poster Presentation 1B-52

**Enzymatic Activities of *Ceriporiopsis subvermispora***

**Acting on Sugarcane Bagasse**

Adilson R. Gonçalves^1^, Sirlene M. Costa^1^ and Elisa Esposito^2^

^1^Depto. Biotecnologia - FAENQUIL, CP 116, CEP 12.600-970 Lorena,
SP-Brazil

^2^Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes,
Brazil

Phone: 55-12-31595033; Fax: 55-12-5533165; E-mail:
<adilson@debiq.faenquil.br>

URL: <http://www.debiq.faenquil.br/adilson>

Sugarcane bagasse was pre-treated with the white-rot fungus
*Ceriporiopsis subvermispora* from 7 to 60 days. The aim of the work was
the production of pulps or modified fibers from the pre-treated bagasse.
Enzymatic activities for lignin peroxidase (LiP), manganese peroxidase
(MnP), laccase (Lac) and xylanase (Xyl) were determined. The table shows
the preliminar results for Xyl and MnP production in experiments in
which the inoculum charge was varied. Xyl increased during the
fermentation and also by the increasing of inoculum charge. On the other
hand, MnP has a maximum of production until 30 days and seems to be not
influenced by the inoculum charge. These parameters are useful to
determine the better fermentation conditions. Additional results on Lac
and LiP production will be presented.

+----------+--------+----------+-----------+----------+--------------+
| Inoculum | 7 days | 15 days  | 30 days   | 45 days  | 60 days      |
|          |        |          |           |          |              |
| charge   |        |          |           |          |              |
| (mL)     |        |          |           |          |              |
+----------+--------+----------+-----------+----------+--------------+
|          |        |          | Xylanase  |          |              |
+----------+--------+----------+-----------+----------+--------------+
| 100      | 51 1   | 118 5    | 4787 269  | 3542 195 | 4847 446     |
+----------+--------+----------+-----------+----------+--------------+
| 250      | 1065   | 1209 90  | 4384 189  | 4161 480 | 6678 479     |
|          | 30     |          |           |          |              |
+----------+--------+----------+-----------+----------+--------------+
| 500      | 526 25 | 1661 93  | 4691 190  | 5504 316 | 6709 447     |
+----------+--------+----------+-----------+----------+--------------+
| 750      | 1094   | 5273 94  | 4931 295  | 2314 196 | 5960 514     |
|          | 20     |          |           |          |              |
+----------+--------+----------+-----------+----------+--------------+
|          |        |          | MnP       |          |              |
+----------+--------+----------+-----------+----------+--------------+
| 100      | \-     | \-       | 310 29    | 82 3     | 19 2         |
+----------+--------+----------+-----------+----------+--------------+
| 250      | 294 12 | 6 1      | 215 20    | 23 2     | 28 1         |
+----------+--------+----------+-----------+----------+--------------+
| 500      | 339 13 | 101 5    | 30 3      | 97 3     | 6 1          |
+----------+--------+----------+-----------+----------+--------------+
| 750      | 294 12 | 112 9    | 118 12    | 30 2     | 27 2         |
+----------+--------+----------+-----------+----------+--------------+

\[Financial support from FAPESP and CNPq\]