**SAMPLING AND ANALYSIS PLAN (SAP)**

**GUIDANCE AND TEMPLATE**

**VERSION 1, EPA ANALYTICAL SERVICES USED**

**R9QA/001.1**

**April, 2000**

This Sampling and Analysis Plan (SAP) guidance and template is intended
assist organization in documenting the procedural and analytical
requirements for a one‑time or time‑limited projects involving the
collection of water, soil, sediment, or biological samples taken to
characterize areas of potential environmental contamination. It
combines, in a short form, the basic elements of a Quality Assurance
Project Plan (QAPP) and a Field Sampling Plan (FSP). Once prepared and
approved it will meet the requirements for any U.S. Environmental
Protection Agency (EPA) Region 9 funded project in which environmental
measurements are to be taken.

The format is designed to accommodate projects of limited scope and is
predicated on the assumption that the work will be going to the Region 9
Laboratory or other EPA funded laboratory rather than a private
laboratory under contract to the organization writing the SAP. If a
non-EPA laboratory will be used, Version 2 (R9QA/002) of this template
should be used. It is intended to be used for projects generating a
limited number of samples (note that these samples may be analyzed by
more than one analytical method, e.g., metals, nitrate, and pesticides)
which will be collected over a period of not more than 14 days. This
template is not intended to be used for on-going monitoring events, or
for remediation or removal activities. Exceptions to all of these
requirements will be considered on a case by case basis, but they should
be discussed with Region 9 QA Office staff before the template is used
and before the SAP is submitted for approval. The primary programs using
this guidance are expected to be (but not limited to) Superfund Site
Assessment projects, Brownfields projects, tribal projects, Clean Water
Act projects, and RCRA (Resource Conservation and Recovery Act)
projects. It can be used by state, muncipal and local, contractor,
non-profit, organizations and by EPA staff. Not all sections will apply
to all organizations.

This guide is to be used as a template. It provides item‑by‑item
instructions for creating a SAP and includes example language which can
be used with or without modification. More complete information is
provided for completing the sections describing sampling procedures,
since it is implicit in the use of this template that EPA\'s analytical
procedures are acceptable to the organization generating the plan. If
information on these sampling procedures is needed, QA Office staff
should be contacted. If these procedures will not meet project needs,
the organization can substitute a description of its own sampling
procedures or provide copies of its sampling SOPs. Other alternatives
should be discussed with QA Office staff.

If the sections in the SAP are appropriate for the project, they may be
used verbatim or modified as needed to reflect project‑ and
sampling‑specific requirements. An electronic version of the template in
WordPerfect is available and it is expected that it, rather than a
hardcopy version of the template, will be used to prepare the SAP.

The U.S. EPA Region 9 Quality Assurance Office is available to provide
assistance in completing the SAP. Contact Dr. David Taylor at
415‑744‑1497, or Ms. Gail Jones at 415-744-1498.

The format of the template is as follows:

All informational and instructional language will be shaded and is to be
deleted from the final SAP.

Tutorial information is presented in *italic* type. This information
includes definitions and background information pertaining to a given
section of the SAP and is to be deleted from the final document.

Specific instructions are given inside brackets \[in normal type\]. The
brackets and the instructions inside the brackets should be deleted from
the final document.

Suggested text which may be included in the SAP is presented in normal
type. This text can be used, modified, or deleted depending on the
nature of the project. For example, delete the discussion of soil
sampling if only groundwater will be sampled.

If the use of an SOP is appropriate, the SOP should be included as an
appendix to the final SAP and referenced in the appropriate section of
the SAP.

An underlined blank area \_\_\_\_\_\_\_\_\_\_\_ indicates that text
should be added. The underlined area is not meant to imply how much text
should be added, only that it is a place that the plan writer should be
adding information. Adjust the space provided as necessary to completely
address each section. The underlined area should be deleted (i.e., do
not underline added text).

Examples or choices are given in \[brackets\] following the blank. If
appropriate, select one and delete the others.

If a given section does not apply, it is recommneded (but not required)
that the section state \"Not applicable,\" or \"Does not apply,\" under
the section heading. By not deleting the section, the writer avoids
having to renumber sections. However, sections can be removed altogether
and the remaining sections renumbered if the organization prefers.

Sampling and Analysis Plan for

\[Title of Project\]

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\[Name and Address of Organization Here\]

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Date

\[Name of Organization\] Project Manager
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\[Name of Organization\] QA Manager
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+---------------------------------+---+-----------------------------+---+
| **For EPA use:**                |   |                             |   |
+---------------------------------+---+-----------------------------+---+
| Approved by EPA Project         |   | Date:                       |   |
| Manager:                        |   |                             |   |
|                                 |   | \_\_\_\_\_\_\_\_\_\_\_\     |   |
| \_\_\_\_\_\_\_\_\_\             |   | _\_\_\_\_\_\_\_\_\_\_\_\_\_ |   |
| _\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ |   |                             |   |
+---------------------------------+---+-----------------------------+---+
| Expedited Review?  Yes         |   |  No                        |   |
+---------------------------------+---+-----------------------------+---+
| Received by QA Office:          |   | Date:                       |   |
|                                 |   |                             |   |
| \_\_\_\_\_\_\_\_\_\_\           |   | \_\_\_\_\_\_\_\_\_\_\_\     |   |
| _\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ |   | _\_\_\_\_\_\_\_\_\_\_\_\_\_ |   |
+---------------------------------+---+-----------------------------+---+
| Reviewed by:                    |   | Date:                       |   |
|                                 |   |                             |   |
| \_\_\_\_\_\_\_\_\_\_\           |   | \_\_\_\_\_\_\_\_\_\_\_\     |   |
| _\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ |   | _\_\_\_\_\_\_\_\_\_\_\_\_\_ |   |
+---------------------------------+---+-----------------------------+---+
| Approved:                       |   | Date:                       |   |
|                                 |   |                             |   |
| \_\_\_\_\_\_\_\_\_\_\           |   | \_\_\_\_\_\_\_\_\_\_\_\     |   |
| _\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ |   | _\_\_\_\_\_\_\_\_\_\_\_\_\_ |   |
|                                 |   |                             |   |
| Region 9 Quality Assurance      |   |                             |   |
| Manager                         |   |                             |   |
+---------------------------------+---+-----------------------------+---+

  --------------------------------- --- ----------------------------- ---
                                                                      

  --------------------------------- --- ----------------------------- ---

**TABLE OF CONTENTS**

**1.0 INTRODUCTION 9**

**1.1 Site Name or Sampling Area 9**

**1.2 Site or Sampling Area Location 9**

**1.3 Responsible Agency 9**

**1.4 Project Organization 10**

**1.5 Statement of the Specific Problem 11**

**2.0 BACKGROUND 12**

**2.1 Site or Sampling Area Description 12**

**2.2 Operational History 13**

**2.3 Previous Investigations and Regulatory Involvement 14**

**2.4 Geological Information 14**

**2.5 Environmental and/or Human Impact 15**

**3.0 PROJECT DATA QUALITY OBJECTIVES 15**

**3.1 Project Task and Problem Definition 16**

**3.2 Data Quality Objectives (DQOs) 16**

**3.3 Data Quality Indicators (DQIs) 18**

**3.4 Data Review and Validation 21**

**3.5 Data Management 23**

**3.6 Assessment Oversight 23**

**4.0 SAMPLING RATIONALE 24**

**4.1 Soil Sampling 24**

**4.2 Sediment Sampling 25**

**4.3 Water Sampling 25**

**4.4 Biological Sampling 26**

**4.4.1 26**

**4.4.2 27**

**5.0 REQUEST FOR ANALYSES 27**

**5.1. Analyses Narrative 28**

**6.0 FIELD METHODS AND PROCEDURES 29**

**6.1 Field Equipment 30**

**6.1.1 List of Equipment Needed 30**

**6.1.2 Calibration of Field Equipment 30**

**6.2 Field Screening 30**

**6.3 Soil 31**

**6.3.1 Surface Soil Sampling 31**

**6.3.2 Subsurface Soil Sampling 33**

**6.4 Sediment Sampling 36**

**6.5 Water Sampling 37**

**6.5.1 Surface Water Sampling 37**

**6.5.2 Groundwater Sampling 39**

**6.5.2.1 Water-Level Measurements 39**

**6.5.2.2 Purging 40**

**6.5.2.3 Well Sampling 42**

**6.6 Biological Sampling 45**

**6.6.1 Biological Sampling for Chemical Analysis 45**

**6.6.1.1 Fish Samples 46**

**6.6.1.2 Foliage Samples 46**

**6.6.2 47**

**6.7 Decontamination Procedures 47**

**7.0 SAMPLE CONTAINERS, PRESERVATION AND STORAGE 49**

**7.1 Soil Samples 49**

**7.2 Sediment Samples 51**

**7.3 Water Samples 52**

**7.4 Biological Samples 55**

**7.4.1 Fish Samples 55**

**7.4.2 Foliage Samples 55**

**8.0 DISPOSAL OF RESIDUAL MATERIALS 55**

**9.0 SAMPLE DOCUMENTATION AND SHIPMENT 59**

**9.1 Field Notes 59**

**9.1.1 Field Logbooks 59**

**9.1.2 Photographs 61**

**9.2 Labeling 61**

**9.3 Sample Chain-Of-Custody Forms and Custody Seals 62**

**9.4 Packaging and Shipment 64**

**10.0 QUALITY CONTROL 66**

**10.1.1 Assessment of Field Contamination (Blanks) 66**

**10.1.1.1 Equipment Blanks 67**

**10.1.1.2 Field Blanks 70**

**10.1.1.3 Trip Blanks 71**

**10.1.1.4 Temperature Blanks 73**

**10.1.2 Assessment of Sample Variability (Field Duplicate or Co-located
Samples) 73**

**10.2 Background Samples 76**

**10.3 Field Screening, Confirmation, and Split Samples 77**

**10.3.1 Field Screening Samples 78**

**10.3.2 Confirmation Samples 78**

**10.3.3 Split Samples 79**

**10.4 Laboratory Quality Control Samples 80**

**11.0 FIELD VARIANCES 82**

**12.0 FIELD HEALTH AND SAFETY PROCEDURES 83**

**Table of Contents**

**Table 5-1\
REQUEST FOR ANALYTICAL SERVICES\
MATRIX = SOIL** 84

**Table 5-2\
REQUEST FOR ANALYTICAL SERVICES\
MATRIX = SEDIMENT** 86

**Table 5-3\
REQUEST FOR ANALYTICAL SERVICES\
MATRIX = GROUNDWATER** 87

**Table 5-4\
REQUEST FOR ANALYTICAL SERVICES\
MATRIX = SURFACE WATER** 88

**Table 5-5\
REQUEST FOR ANALYTICAL SERVICES\
MATRIX = FISH** 90

**1.0 INTRODUCTION**

\[This section should include a brief description of the project,
including the history, problem to be investigated, scope of sampling
effort, and types of analyses that will be required. These topics will
be covered in depth later so do not include a detailed discussion
here.\]

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**1.1 Site Name or Sampling Area**

\[Provide the most commonly used name of the site or sampling area.\]

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**1.2 Site or Sampling Area Location**

\[Provide a general description of the region, state or tribal area in
which the site or sampling area is located. Detailed sampling location
information should be provided later in Section 2\]

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**1.3 Responsible Agency**

\[Provide a description of the organization conducting the sampling.\]

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**1.4 Project Organization**

\[Fill out the table. Provide the name and phone number(s) of the
person(s) and/or contractor working on the sampling project as listed in
the table. The table can be modified to include titles or positions
appropriate to the specific project. Delete personnel or titles not
appropriate to the project\]

*It should be noted that it is the responsibility of the Quality
Assurance (QA) Officer to oversee the implementation of the Sampling and
Analysis Plan or QA Plan if one has been prepared, including whether
specified quality control (QC) procedures are being followed as
described. Ideally, this individual should discuss QA issues with the
Project Manager, but should not be involved in the data
collection/analysis/interpretation/reporting process except in a review
or oversight capacity. If the project is small, another technical person
may fulfill this role.*

  --------------------------------- ---------------------- ---------------
  **Title/Responsibility**          **Name**               **Phone
                                                           Number**

  **EPA Project Manager**                                  

  **Project Manager**                                      

  **Staff**                                                

                                                           

  **Quality Assurance Manager**                            

                                                           

  **Contractor (Company Name)**                            

  **Contractor Staff**                                     

                                                           

                                                           

                                                           
  --------------------------------- ---------------------- ---------------

**1.5 Statement of the Specific Problem**

\[In describing the problem, include historical, as well as recent,
information and data that may be relevant. List and briefly outline
citizens\' complaints, public agency inspections, and existing data.
Include sources of information if possible.\]

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**2.0 BACKGROUND**

*This section provides an overview of the location, previous
investigations, and the apparent problem(s) associated with the site or
sampling area.*

\[Provide a brief description of the site or sampling area, including
chemicals used on the site, site history, past and present operations or
activities that may have contributed to the suspected contamination,
etc.\]

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**2.1 Site or Sampling Area Description** \[Fill in the blanks.\]

\[Two maps of the area should be provided: the first (Figure 2.1), on a
larger scale, should place the area within its geographic region; the
second (Figure 2.2), on a smaller scale, should mark the sampling site
or sampling areas within the local area. Additional maps may be
provided, as necessary, for clarity. Maps should include a North arrow,
groundwater flow arrow (if appropriate), buildings or former buildings,
spill areas, etc. If longitude or latitude information is available,
such as from a Global Positioning System (GPS), provide it. Sampling
locations can be shown in Figure 2.2.\]

The site or sampling area occupies \_\_\_\_\_\_\_\_\_\_ \[e.g., acres or
square feet\] in a \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[e.g., urban,
commercial, industrial, residential, agricultural, or undeveloped\]
area. The site or sampling area is bordered on the north by
\_\_\_\_\_\_\_\_\_\_\_, on the west by \_\_\_\_\_\_\_\_\_\_\_\_\_\_, on
the south by \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_, and on the east by
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_. The specific location of the site or
sampling area is shown in Figure 2.2.

*The second paragraph (or set of paragraphs) should describe historic
and current on-site structures and should be consistent with what is
presented in Figure 2.2.*

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\[Depending on the nature of the project, some of the following sections
may not be applicable. If this is the case, do not delete the section.
Instead enter \"Not Applicable\" or other text to indicate that the
section does not apply or that the information is not available.\]

**2.2 Operational History**

\[As applicable, describe in as much detail possible (i.e., use several
paragraphs) the past and present activities at the site or sampling
area. The discussion might include the following information: a
description of the owner(s) and/or operator(s) of the site or areas near
the site, the watershed of interest, the sampling area, etc. \[Present
this information chronologically\]; a description of past and current
operations or activities that may have contributed to suspected
contamination; a description of the processes involved in the
operation(s) and the environmentally detrimental substances, if any,
used in the processes; a description of any past and present waste
management practices. If a waste site, were/are hazardous wastes
generated by one or more of the processes described earlier? If so, what
were/are they, how and where were/are they stored on the site or
sampling area, and where were/are they ultimately disposed of? If an
ecosystem, what point and non-point sources may have affected the river,
stream, lake or watershed? Approximately what level of contamination is
expected (high = \>10 mg/L or mg/Kg; medium = 10 mg/L or mg/Kg to 10
ug/L or ug/Kg; low = \<10 ug/L or ug/Kg)?\]

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**2.3 Previous Investigations and Regulatory Involvement** \[if
applicable\]

\[Summarize all previous sampling efforts at the site or sampling area.
Include the sampling date(s); name of the party (ies) that conducted the
sampling; local, tribal, state or federal government agency for which
the sampling was conducted; a rationale for the sampling; the type of
media sampled (e.g., soil, sediment, water); laboratory methods that
were used; and a discussion of what is known about data quality and
usability. The summaries should be presented in subsections according to
the media that were sampled (e.g., soil, water, etc.) and
chronologically within each medium. Attach reports or summary tables of
results or include in appendices if necessary.\]

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**2.4 Geological Information** \[groundwater sampling only\]

\[Provide a description of the hydrogeology of the area. Indicate the
direction of groundwater flow, if known.\]

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**2.5 Environmental and/or Human Impact**

\[Discuss what is known about the possible and actual impacts of the
possible environmental problem on human health or the environment.\]

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**3.0 PROJECT DATA QUALITY OBJECTIVES**

*Data Quality Objectives (DQOs) are qualitative and quantitative
statements for establishing criteria for data quality and for developing
data collection designs. This section is crucial to SAP approval, since
it defines what the data will be used for and what quality of data are
needed to make decisions. EPA's* *Guidance for the Data Quality
Objectives Process (EPA QA/G-4, Final, September, 1994)* *should be
consulted for more information.*

*DQOs should cover the following items:*

* Concisely describe the problem to be studied.*

> * Identify what questions the study will attempt to resolve, and what
> actions (decisions) may result*
>
> * Identify the information that needs to be obtained and the
> measurements that need to be taken to resolve the decision statement*
>
> * Define study boundaries and when and where data should be
> collected.*

*Most projects utilizing this template are small so that defining action
levels and data quality indicators (DQIs) for the field and laboratory
measurements used on the project are sufficient. DQIs define criteria
for calibration and quality control (QC) for field and laboratory
methods. DQIs are discussed more thoroughly below.*

**3.1 Project Task and Problem Definition**

\[Describe the purpose of the environmental investigation in qualitative
terms and how the data will be used. Generally, this discussion will be
brief and generic. Include all measurements to be made on an analyte
specific basis in whatever medium (soil, sediment, water, etc.) is to be
sampled. This discussion should relate to how this sampling effort will
support the specific decisions described in Section 3.2., DQOs, below.
If this project is for the purpose of scoring a site using the Hazardous
Ranking System under Superfund, refer to Appendix 1.\]

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**3.2 Data Quality Objectives (DQOs)**

*Data quality objectives (DQOs) are quantitative and qualitative
criteria that establish the level of uncertainty associated with a set
of data. They answer the question: How sure are you that the value of
the data are what the analyses have determined them to be? All the
elements of the sampling event, from the sampling design through
laboratory analysis and reporting, affect the quality of the data. The
project manager or other decision maker identified earlier in the
project organization section must make the decision as to what level of
uncertainty is acceptable or appropriate. Depending on what the
contaminants of concern are, what effect they may have on human and
environmental health, and what levels are of concern, data may need to
be legally defensible or capable of answering a simple
"presence-absence" question. In addition to DQOs, data quality is also
defined by data quality indicators, which are discussed in the next
section. More sophisticated DQO discussions involve defining null
testing hypotheses and confidence intervals. These should be considered
depending on project decision making needs, but such discussions are
generally not expected in one-time event SAPs.*

This section should describe decisions to be made based on the data and
provide criteria on which these decisions will be made. Inclusion of one
or more tables is recommended; tables which contain all the contaminants
of concern, their associated action levels, and the source of the action
level (regulation, health based criteria, water quality standards, etc.)
If a contaminant does not have an action level, or will not be used in
decision making, the text should discuss how the data for that
contaminant will be used. The use of "\...if\...then" statements is
recommended. Note that decisions do not have to involve regulatory or
legal action (and for one-time SAP projects, few are expected to). Some
examples: "If any contaminant is found above the levels specified in
Table \_\_\_, but below 10X that level, then it will be monitored on a
yearly basis. If a contaminant is found above 10X the action level, then
corrective action in the form of source investigation will be
initiated," or: "If contaminants are found above the action level, then
a Phase II Site Investigation will be initiated," or: "If contamination
is found above the action level for compound X, then it will be
bioremediated in situ. Locations containing metals above the action
limit will be excavated and retested."

\[Discuss Data Quality Objectives, action levels, and decisions to be
made based on the data here.\]

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**3.3 Data Quality Indicators (DQIs)**

*Data quality indicators (accuracy, precision, completeness,
representativeness, comparability, and method detection limits) refer to
quality control criteria established for various aspects of data
gathering, sampling, or analysis activity. In defining DQIs specifically
for the project, the level of uncertainty associated with each
measurement is defined.*

*The values that are to be assigned to the quantitative data quality
indicators (accuracy, precision, completeness) and statements concerning
the qualitative indicators (representativeness and comparability) are
determined by the answers to the questions in Section 3.2. If EPA
schedules the analytical work, it generally defines DQIs for its
laboratories, but the organization writing the SAP should include the
appropriate tables to make the SAP complete and to acknowledge that it
understands and agrees to the DQIs which will be used for the project.
DQIs for work performed by the Contract Laboratory Program will be
defined in the organic and inorganic statements of work (SOWs). The QA
Office should be consulted to see what information should be included in
EPA lead projects one-time SAPs. Definition of the different terms are
provided below:*

> *Accuracy is the degree of agreement of a measurement with a known or
> true value. To determine accuracy, a laboratory or field value is
> compared to a known or true concentration. Accuracy is determined by
> such QC indicators as: matrix spikes, surrogate spikes, laboratory
> control samples (blind spikes) and performance samples.*
>
> *Precision is the degree of mutual agreement between or among
> independent measurements of a similar property (usually reported as a
> standard deviation \[SD\] or relative percent difference \[RPD\]).
> This indicator relates to the analysis of duplicate laboratory or
> field samples. An RPD of \<20% for water and \<35% for soil, depending
> upon the chemical being analyzed, is generally acceptable. Typically
> field precision is assessed by co-located samples, field duplicates,
> or field splits and laboratory precision is assessed using laboratory
> duplicates, matrix spike duplicates, or laboratory control sample
> duplicates).*
>
> *Completeness is expressed as percent of valid usable data actually
> obtained compared to the amount that was expected. Due to a variety of
> circumstances, sometimes either not all samples scheduled to be
> collected can be collected or else the data from samples cannot be
> used (for example, samples lost, bottles broken, instrument failures,
> laboratory mistakes, etc.). The minimum percent of completed analyses
> defined in this section depends on how much information is needed for
> decision making. Generally, completeness goals rise the fewer the
> number of samples taken per event or the more critical the data are
> for decision making. Goals in the 75-95% range are typical.*
>
> *Representativeness is the expression of the degree to which data
> accurately and precisely represent a characteristic of an
> environmental condition or a population. It relates both to the area
> of interest and to the method of taking the individual sample. The
> idea of representativeness should be incorporated into discussions of
> sampling design. Representativeness is best assured by a comprehensive
> statistical sampling design, but it is recognized that this is usually
> outside the scope of most one-time events. Most one time SAPs should
> focus on issues related to judgmental sampling and why certain areas
> are included or not included and the steps being taken to avoid either
> false positives or false negatives.*
>
> *Comparability expresses the confidence with which one data set can be
> compared to another. The use of methods from EPA or "Standard Methods"
> or from some other recognized sources allows the data to be compared
> facilitating evaluation of trends or changes in a site, a river,
> groundwater, etc. Comparability also refers to the reporting of data
> in comparable units so direct comparisons are simplified (e.g., this
> avoids comparison of mg/L for nitrate reported as nitrogen to mg/L of
> nitrate reported as nitrate, or ppm vs. mg/L discussions).*
>
> *Detection Limit(s) (usually expressed as method detection limits
> (MDLs) or Quantitation Limit(s) for all analytes or compounds of
> interest for all analyses requested must be included in this section.
> These limits should be related to any decisions that will be made as a
> result of the data collection effort. A critical element to be
> addressed is how these limits relate to any regulatory or action
> levels that may apply.*

*DQI tables are available from the QA Office for most routinely ordered
methods. These tables can be attached to the SAP and referenced in this
section. If an organization, its contractor, or its laboratory wish to
use different limits or acceptance criteria, the table should be
modified accordingly. SOPs should be included for methods not covered by
the DQI tables or they can be submitted in lieu of the tables. Due to
resource constraints, generally only the DQI aspects of these SOPs will
be evaluated.*

\[Provide or reference DQI tables here\]

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**3.4 Data Review and Validation**

This section should discuss data review, including what organizations or
individuals will be responsible for what aspects of data review and what
the review will include. Region 9 has adopted a tiered approach to data
review, and any data generated by the EPA analytical system is subject
to this system. If EPA will be used, the SAP should specify what tier is
to be used for this project. The SAP should discuss the process by which
the evaluation of data quality will be made. Describe how data that do
not meet data quality objectives will be designated. Assistance is
available from the QA Office.

If data need to be legally defensible, data packages and data validation
may be required. (EPA defines validation as a 3rd party review of all
laboratory data based on strict protocols). Data reviewed include raw
data such as standards log books, extractions logs, instrument print
outs, chromatograms (if applicable), mass spectra (if applicable), etc.
Calibration data, sample analysis data, and quality control data are all
evaluated.) Details on validation are available from the QA Office, but
a brief summary follows:

1.  *Tier 1A involves a review of the QC data for the project, with up
    to 10% of the data being validated. Validation candidates are chosen
    based on the initial screening or higher level hits.*

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1.  *Tier 1B involves a review using the EPA's automated data validation
    system, CADRE. This review is limited to data in the correct
    electronic format and only covers analyses for volatile organic
    compounds, semivolatile organic compounds, organochlorine
    pesticides, metals, and cyanide.*

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1.  *Tier 2 involves a selected validation based on several factors
    which should be defined in the DQOs for the project. Candidates
    might be a specific area within the sampling area, specific analytes
    or analyses of concern critical to decision making, potential costs
    of certain types of resampling, or some other factor.*

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1.  *Tier 3 involves a traditional full validation.*

*There is no requirement that all data adhere to the same Tier; the
project can mix and match depending on DQOs. If a project involves a mix
of EPA analytical services and services contracted by the organization
writing the SAP, this section should describe how the data from the
non-EPA source will be reviewed and/or validated. It is recommended
that, if validation will be a part of data review, that SOP(s) from the
organization which will perform the validation be attached.
Alternatively, the QA Office Data Review Team can be requested to review
the validating organization's validation SOP on a generic basis. Once
reviewed to ensure it is consistent with Region 9 protocols, it can be
referenced in all future SAPs.*

\[Discuss data review and data validation here including what
organizations or individuals will be responsible for what aspects of
data review and what the review will include. This section should also
discuss how data that do not meet data quality objectives will be
designated, flagged, or otherwise handled. Possible corrective actions
associated with the rejection of data; such as not using data in
decision making, reanalysis, resampling, no action but monitor the data
more closely next quarter, etc.; also need to be addressed\]

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**3.5 Data Management**

\[Provide a list of the steps that will be taken to ensure that data are
transferred accurately from collection to analysis to reporting. Discuss
the measures that will be taken to review the data collection processes,
including field notes or field data sheets; to obtain and review
complete laboratory reports; and to review the data entry system,
including its use in reports. A checklist is acceptable.\]

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**3.6 Assessment Oversight**

\[Describe the procedures which will be used to implement the QA
Program. This would include oversight by the Quality Assurance Manager
or the person assigned QA responsibilities. Indicate how often a QA
review of the different aspects of the project, including audits of
field and laboratory procedures, use of performance samples, etc., will
take place. Describe what authority the QA Manager or designated QA
person has to ensure that identified field and analytical problems will
be corrected and the mechanism by which this will be accomplished.\]

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**4.0 SAMPLING RATIONALE**

*For each sampling event, the SAP must describe the sampling locations,
the media to be sampled, and the analytes of concern at each location. A
rationale should then be provided justifying these choices. The
following sections are subdivided on a media specific basis among soil,
sediment, water, and biological media. Other media should be added as
needed. This section is crucial to plan approval and should be closely
related to previously discussed DQOs.*

**4.1 Soil Sampling**

\[Provide a general overview of the soil sampling event. Present a
rationale for choosing each sampling location at the site or sampling
area and the depths at which the samples are to be taken, if relevant.
If decisions will be made in the field, provide details concerning the
criteria that will be used to make these decisions (i.e., the decision
tree to be followed). List the analytes of concern at each location and
provide a rationale for why the specific chemical or group of chemicals
(e.g., organochlorine pesticides) were chosen. Include sampling
locations in Figure 2.2 or equivalent.\]

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**4.2 Sediment Sampling**

\[Provide a general overview of the sediment sampling event. Present a
rationale for choosing each sampling location at the site or sampling
area and the depths or area of the river, stream or lake at which the
samples are to be taken, if relevant. If decisions will be made in the
field, provide details concerning the criteria that will be used to make
these decisions (i.e., the decision tree to be followed). List the
analytes of concern at each location and provide a rationale for why the
specific chemical or group of chemicals (e.g., organochlorine
pesticides) were chosen. Include sampling locations in Figure 2.2 or
equivalent.\]

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**4.3 Water Sampling**

\[Provide a general overview of the water sampling event. For
groundwater, describe the wells to be sampled or how the samples will be
collected (e.g., hydro punch), including the depths at which the samples
are to be taken. For surface water, describe the depth and nature of the
samples to be collected (fast or slow moving water, stream traverse,
etc.). Present a rationale for choosing each sampling location or
sampling area. If decisions will be made in the field, provide details
concerning the criteria that will be used to make these decisions (i.e.,
the decision tree to be followed). List the analytes of concern at each
location and provide a rationale for why the specific chemical or group
of chemicals (e.g., organochlorine pesticides) were chosen. For
microbiological samples, discuss the types of bacterial samples being
collected. Include sampling locations in Figure 2.2 or equivalent.\]

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**4.4 Biological Sampling**

\[For each of the two types of events identified, provide a general
overview of the biological sampling event. Present a rationale for
choosing each sampling location at the site or sampling area, including
the parameters of interest at each location. If decisions will be made
in the field, provide details concerning the criteria that will be used
to make these decisions (i.e., the decision tree to be followed).

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**4.4.1** Biological Samples for Chemical Analysis

\[For sampling where flora or fauna will be analyzed for the presence of
a chemical (e.g. fish collected for tissue analysis), explain why the
specific chemical or group of chemicals (e.g., metals, organochlorine
pesticides, etc.) is included. List the types of samples to be collected
(e.g., fish, by species or size, etc.) and explain how these will be
representative. Include sampling locations in Figure 2.2 or
equivalent.\]

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**4.4.2** Biological Sample for Species Identification and Habitat
Asessment

\[If the purpose of the sampling is to collect insects or other
invertebrates or to make a habitat assessment, a rationale for the
sampling to take place should be provided. For example, what species are
of interest and why?\]

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**5.0 REQUEST FOR ANALYSES**

*EPA schedules analytical support for the project depending on several
factors including the analyses requested, analytes of concern,
turnaround times, available resources, available laboratories, etc.
Ideally, these options include Contract Laboratory Program Analytical
Services (CLPAS) (Superfund and Brownfields projects only), the Region 9
Laboratory in Richmond, California, and commercial laboratories under
contract to Region 9. The Region 9 QA Office determines where samples
will be sent. The SAP should not contain this information unless the
Regional Sample Control Coordinator has indicated in advance where the
samples will go. If samples will be sent through Region 9 and also to
another organization with which the sampling organization has
established a contract, this section should make it clear which samples
go to EPA and which do not. Field analyses for pH, conductivity,
turbidity, or other field tests should be discussed in the sampling
section. Field measurements in a mobile laboratory (for example, the
Field Analytical Support Program (FASP) laboratory) should be discussed
here and differentiated from samples to be sent to a fixed laboratory.
Field screening tests (for example, immunoassay tests) should be
discussed in the sampling section, but the confirmation tests should be
discussed here and the totals included in the tables.*

\[Complete the following narrative subsection concerning the analyses
for each matrix. In addition, fill in Tables 5-1 through 5-5, as
appropriate. A Request for Analyses (RFA) table must be included for
each matrix to be sampled. Example RFA tables for each matrix are
included with this SAP template. Each table must be completed to list
analytical parameters for each type of sample. Include information on
container types, sample volumes, preservatives, special handling and
analytical holding times for each parameter. Quality Control (QC)
samples (blanks, duplicates, splits, and laboratory QC samples, see
Section 10 for description) should be indicated in the column titled
\"Special Designation.\" The extra volume needed for laboratory QC
samples (for water samples only) should be noted on the table. The
tables provided do not have to be used, but the critical information
concerning the number of samples, matrix, analyses requested and QC
sample identification must be provided in some form or work cannot be
scheduled by EPA. The selected analyses must be consistent with earlier
discussions concerning DQOs and analytes of concern. DQI information for
the methods should be discussed in Section 10 on quality control
requirements.\]

**5.1. Analyses Narrative**

\[Fill in the blanks. Provide information for each analysis requested.
Delete the information below as appropriate. Include any special
requests, such as fast turn‑around time (2 weeks or less), specific QC
requirements, or modified sample preparation techniques in this section.
An example of the narrative follows.\]

As enumerated in Table 5.\_\_, \_\_\_\_\[Indicate matrix, e.g., soil\]
samples will be taken at \_\_\_\_\[State total number of locations\]
locations. \_\_\_\_\_\_\_\_\[\"Single\" or \"Double\" depending on
laboratory requirements\] volume \_\_\_\_\[matrix\] samples collected at
the following sample locations will be identified for use as laboratory
QC samples: \_\_\_\_\_\[QC sample numbers\]. Duplicate
\_\_\_\_\[matrix\] samples will be collected at the following sample
locations: \_\_\_\_\_

\[Indicate locations for sample duplicates\]. \[A rationale for the
choice of sample locations for QC should be provided in Section 10\].

As shown in Table 5.\_\_\_, each \_\_\_\_\_\[indicate matrix\] sample
(including laboratory QC samples) will be analyzed for
\_\_\_\_\_\_\[describe requested analyses\].

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**6.0 FIELD METHODS AND PROCEDURES**

*In the general introductory paragraph to this section, there should be
a description of the methods and procedures that will be used to
accomplish the sampling goals, e.g., \"\...collect soil, sediment and
water samples.\" It should be noted that personnel involved in sampling
must wear clean, disposable gloves of the appropriate type. The sampling
discussion should track the samples identified in Section 4.0 and
Table(s) 5-1, 5-2, 5-3, or 5-4. A general statement should be made that
refers to the sections containing information about sample tracking and
shipping (Section 7). Provide a description of sampling procedures.
Example procedures are provided below, but the organization's own
procedures can be used instead. In that case, attach a copy of the
applicable SOP. Some sampling procedures are available from EPA. Contact
the QA Office or visit the Region 9 laboratory's web page.*

**6.1 Field Equipment**

**6.1.1 List of Equipment Needed**

\[List all the equipment that will be used in the field to collect
samples, including decontamination equipment, if required. Discuss the
availability of back‑up equipment and spare parts.\]

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**6.1.2 Calibration of Field Equipment**

\[Describe the procedures by which field equipment is prepared for
sampling, including calibration standards used, frequency of calibration
and maintenance routines. Indicate where the equipment maintenance and
calibration record(s) for the project will be kept.\]

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**6.2 Field Screening**

*In some projects field screening methods may be used in conjunction
with confirmation samples analyzed in a fixed laboratory. This section
should describe these methods or reference attached SOPs. Soil gas or
immunoassay kits are two examples of common field screening methods.*

\[Describe any field screening methods to be used on the project here
including how samples will be collected, prepared, and analyzed in the
field. Include in an appendix, as appropriate, SOPs covering these
methods. Confirmation of screening results should also be described. The
role of the field screening in decision making for the site should also
be discussed here if it has not been covered previously.\]

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**6.3 Soil**

**6.3.1 Surface Soil Sampling**

\[Use this subsection to describe the collection of surface soil samples
that are to be collected within 6-12 inches of the ground surface.
Specify the method (e.g., hand trowels) that will be used to collect the
samples and use the language below or reference the appropriate sections
of a Soil Sampling SOP.\]

\[If exact soil sampling locations will be determined in the field, this
should be stated. The criteria that will be used to determine sampling
locations, such as accessibility, visible signs of potential
contamination (e.g., stained soils, location of former fuel storage
tank, etc.), and topographical features which may indicate the location
of hazardous substance disposal (e.g., depressions that may indicate a
historic excavation) should be provided.\]

Exact soil sampling locations will be determined in the field based on
accessibility, visible signs of potential contamination (e.g., stained
soils), and topographical features which may indicate location of
hazardous substance disposal (e.g., depressions that may indicate a
historic excavation). Soil sample locations will be recorded in the
field logbook as sampling is completed. A sketch of the sample location
will be entered into the logbook and any physical reference points will
be labeled. If possible, distances to the reference points will be
given.

\[If surface soil samples are to be analyzed for organic (non-volatile
compounds and other analytes, use this paragraph; otherwise delete.\]

Surface soil samples will be collected as grab samples (independent,
discrete samples) from a depth of 0 to \_\_\_inches below ground surface
(bgs). Surface soil samples will be collected using a stainless steel
hand trowel. Samples to be analyzed for volatile organic compounds will
be collected first (see below). Samples to be analyzed for
\_\_\_\_\_\_\_\_\_\_ \[List all analytical methods for soil samples
except for volatile organic compounds\] will be placed in a
sample‑dedicated 1‑gallon disposable pail and homogenized with a trowel.
Material in the pail will be transferred with a trowel from the pail to
the appropriate sample containers. Sample containers will be filled to
the top, taking care to prevent soil from remaining in the lid threads
prior to being closed to prevent potential contaminant migration to or
from the sample. Sample containers will be closed as soon as they are
filled, chilled to 4C if appropriate, and processed for shipment to the
laboratory.

\[If surface soil samples are to be analyzed for volatile organic
compounds (VOCs), use this paragraph; otherwise delete.\]

Surface soil samples for VOC analyses will be collected as grab samples
(independent, discrete samples) from a depth of 0 to \_\_\_\[inches or
feet\] below ground surface (bgs). Surface soil samples will be
collected using a 5 gram Encore sampling device, and will be collected
in triplicate. Samples will be sealed using the Encore sampler and a zip
lock bag or else transferred directly from the sampler into a VOA vial
containing either 10 mLs of methanol or sodium bisulfate solution.
Sample containers will be closed as soon as they are filled, chilled
immediately to 4C before wrapping them in bubble wrap, and processed
them for shipment to the laboratory.

\[For surface soil samples which are not to be analyzed for volatile
compounds, use this paragraph; otherwise delete.\]

Surface soil samples will be collected as grab samples (independent,
discrete samples) from a depth of 0 to \_\_\_\[inches or feet\] below
ground surface (bgs). Surface soil samples will be collected using a
stainless steel hand trowel. Samples will be placed in a
sample‑dedicated 1‑gallon disposable pail and homogenized with a trowel.
Material in the pail will be transferred with a trowel from the pail to
the appropriate sample containers. Sample containers will be filled to
the top, taking care to prevent soil from remaining in the lid threads
prior to being closed to prevent potential contaminant migration to or
from the sample. Sample containers will be closed as soon as they are
filled, chilled if appropriate, and processed for shipment to the
laboratory.

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**6.3.2 Subsurface Soil Sampling**

\[Use this subsection for subsurface soil samples that are to be
collected 12 inches or more below the surface. Specify the method (e.g.,
hand augers) that will be used to access the appropriate depth and then
state the depth at which samples will be collected and the method to be
used to collect and then transfer samples to the appropriate containers
or reference the appropriate sections of a Soil Sampling SOP. If SOPs
are referenced, they should be included in an Appendix.\]

\[If exact soil sampling locations will be determined in the field, this
should be stated. The criteria that will be used to determine sampling
locations, such as accessibility, visible signs of potential
contamination (e.g., stained soils), and topographical features which
may indicate the location of hazardous substance disposal (e.g.,
depressions that may indicate a historic excavation) should be provided.
There should also be a discussion concerning possible problems, such as
subsurface refusal\]

\[Include this paragraph first if exact sampling locations are to be
determined in the field; otherwise delete.\]

Exact soil sampling locations will be determined in the field based on
accessibility, visible signs of potential contamination (e.g., stained
soils), and topographical features which may indicate location of
hazardous substance disposal (e.g., depressions that may indicate a
historic excavation). Soil sample locations will be recorded in the
field logbook as sampling is completed. A sketch of the sample location
will be entered into the logbook and any physical reference points will
be labeled. If possible, distances to the reference points will be
given.

\[If subsurface soil samples are to be analyzed for volatile compounds,
use this paragraph; otherwise delete.\]

Samples to be analyzed for volatile organic compounds will be collected
first. Subsurface samples will be collected by boring to the desired
sample depth using \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[whatever method is appropriate or
available\]. Once the desired sample depth is reached, soil samples for
VOC analyses will be collected as independent, discrete samples. Surface
soil samples will be collected using a 5 gram Encore sampling device,
and will be collected in triplicate. Samples will be sealed using the
Encore sampler and a zip lock bag or else transferred directly from the
sampler into a VOA vial containing either 10 mLs of methanol or sodium
bisulfate solution. Sample containers will be closed as soon as they are
filled, chilled immediately to 4C before wrapping them in bubble wrap,
and processed for shipment to the laboratory.

\[If subsurface soil samples are being collected for other than volatile
organic compounds, use these paragraphs; otherwise delete.\]

Subsurface samples will be collected by boring to the desired sample
depth using
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
\[whatever method is appropriate or available\]. Once the desired sample
depth is reached, the
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[hand‑ or
power‑operated device, such as a shovel, hand auger, trier, hollow‑stem
auger or split‑spoon sampler\] will be inserted into the hole and used
to collect the sample. Samples will be transferred from the
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[sampling device\] to a
sample‑dedicated 1‑gallon disposable pail and homogenized with a trowel.

Material in the pail will be transferred with a trowel from the pail to
the appropriate sample containers. Sample containers will be filled to
the top taking care to prevent soil from remaining in the lid threads
prior to being sealed to prevent potential contaminant migration to or
from the sample. After sample containers are filled, they will be
immediately sealed, chilled if appropriate, and processed for shipment
to the laboratory.

\[Include this as the final paragraph regardless of the analyses for
subsurface soil samples.\]

Excess set‑aside soil from the above the sampled interval will then be
repacked into the hole.

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**6.4 Sediment Sampling**

\[Use this subsection if sediment samples are to be collected. Specify
the method (e.g., dredges) that will be used to collect the samples and
at what depth samples will be collected. Describe how samples will be
homogenized and the method to be used to transfer samples to the
appropriate containers. If a SOP will be followed rather than the
language provided, the SOP should be referenced and included in the
appendix\]

\[If exact sediment sampling locations will be determined in the field,
this should be stated. Describe where sediment samples will be
collected, e.g., slow moving portions of streams, lake bottoms, washes,
etc.\]

Exact sediment sampling locations will be determined in the field, based
on \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
\[Describe the criteria to be used to determine sampling locations\].
Care will be taken to obtain as representative a sample as possible. The
sample will be taken from areas likely to collect sediment deposits,
such as slow moving portions of streams or from the bottom of the lake
at a minimum depth of 2 feet.

Sediment samples will be collected from the well bottom at a depth of
\_\_\_\_\_\_\_ inches using a pre‑cleaned \_\_\_\_\_\_\_\_\_\_ sampler.

\[The final paragraph describes sample homogenization, especially
important if the sample is to be separated into solid and liquid phases,
and container filling. Include this paragraph, or a modified form of it,
for all sediment sampling. It is assumed that sediment samples will not
be analyzed for volatile compounds. If sediment is to be analyzed for
volatile organic compounds, the samples to be analyzed for volatile
compounds should not be homogenized, but rather transferred directly
from the sampler into the sample container. If feasible, an Encore
sampling device should be used.\]

Material in the sampler will be transferred to a sample‑dedicated
1‑gallon disposable pail and homogenized with a trowel. Material from
the pail will be transferred with a trowel from the bucket to the
appropriate sample containers. Sample containers will be filled to the
top taking care to prevent soil from remaining in the lid groves prior
to being sealed in order to prevent potential contamination migration to
or from the sample containers. After sample containers are filled, they
will be immediately sealed, chilled if appropriate, and processed for
shipment to the laboratory.

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**6.5 Water Sampling**

**6.5.1 Surface Water Sampling**

\[Use this subsection if samples are to be collected in rivers, streams,
lakes and reservoirs, or from standing water in runoff collection ponds,
gullies, drainage ditches, etc. Describe the sampling procedure,
including the type of sample (grab or composite - see definitions
below), sample bottle preparation, and project‑specific directions for
taking the sample. State whether samples will be collected for chemical
and/or microbiological analyses. Alternatively, reference the
appropriate sections of attached SOPs.\]

[Grab]{.underline}: Samples will be collected at one time from one
location. The sample should be taken from flowing, not stagnant water,
and the sampler should be facing upstream in the middle of the stream.
Samples will be collected by hand or with a sample bottle holder. For
samples taken at a single depth, the bottle should be uncapped and the
cap protected from contamination. The bottle should be plunged into the
water mouth down and filled 6 to 12\" below the surface of the water. If
it is important to take samples at depths, special samplers (e.g.,
Niskin or Kemmerer Depth Samplers) may be required. After filling the
bottle(s), pour out some sample leaving a headspace of 2.5‑5cm (1‑2in).
For microbiological samples, bottles and caps must be sterile. If
sampling of chlorinated water is anticipated, sodium thiosulfate at a
concentration of 0.1 mL of a 10% solution for each 125 mL (4 oz) of
sample volume must be put into the bottle before it is sterilized.

[Time Composite]{.underline}: Samples are collected over a period of
time, usually 24 hours. If a composite sample is required, a flow‑ and
time-proportional automatic sampler should be positioned to take samples
at the appropriate location in a manner such that the sample can be held
at 4^o^C for the duration of the sampling.

[Spatial Composite]{.underline}: Samples are collected from different
representative positions in the water body and combined in equal
amounts. A Churn Splitter or equivalent device will be used to ensure
that the sample is homogeneously mixed before the sample bottles are
filled. Volatile organic compound samples will be collected as discrete
samples and not composited.

\[If exact surface water sample locations will be determined in the
field, this should be stated. Describe the criteria that will be used to
determine where surface water samples will be collected.\]

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**6.5.2 Groundwater Sampling**

\[This subsection contains procedures for water level measurements, well
purging, and well sampling. Relevant procedures should be described
under this heading with any necessary site‑specific modifications.
Alternatively, reference appropriate SOP(s).\]

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**6.5.2.1 Water‑Level Measurements**

\[The following language may be used as is or modified to meet project
needs.\]

All field meters will be calibrated according to manufacturer\'s
guidelines and specifications before and after every day of field use.
Field meter probes will be decontaminated before and after use at each
well.

If well heads are accessible, all wells will be sounded for depth to
water from top of casing and total well depth prior to purging. An
electronic sounder, accurate to the nearest +/‑ 0.01 feet, will be used
to measure depth to water in each well. When using an electronic
sounder, the probe is lowered down the casing to the top of the water
column, the graduated markings on the probe wire or tape are used to
measure the depth to water from the surveyed point on the rim of the
well casing. Typically, the measuring device emits a constant tone when
the probe is submerged in standing water and most electronic water level
sounders have a visual indicator consisting of a small light bulb or
diode that turns on when the probe encounters water. Total well depth
will be sounded from the surveyed top of casing by lowering the weighted
probe to the bottom of the well. The weighted probe will sink into silt,
if present, at the bottom of the well screen. Total well depths will be
measured by lowering the weighted probe to the bottom of the well and
recording the depth to the nearest 0.1 feet.

Water‑level sounding equipment will be decontaminated before and after
use in each well. Water levels will be measured in wells which have the
least amount of known contamination first. Wells with known or suspected
contamination will be measured last.

**6.5.2.2 Purging**

\[Describe the method that will be used for well purging (e.g.,
dedicated well pump, bailer, hand pump). Reference the appropriate
sections in the Ground Water SOP and state in which Appendix the SOP is
located.\]

\[VERSION A\]

All wells will be purged prior to sampling. If the well casing volume is
known, a minimum of three casing volumes of water will be purged using
the dedicated well pump.

\[VERSION B\]

All wells will be purged prior to sampling. If the well casing volume is
known, a minimum of three casing volumes of water will be purged using a
hand pump, submersible pump, or bailer, depending on the diameter and
configuration of the well. When a submersible pump is used for purging,
clean flexible Teflon tubes will be used for groundwater extraction. All
tubes will be decontaminated before use in each well. Pumps will be
placed 2 to 3 feet from the bottom of the well to permit reasonable draw
down while preventing cascading conditions.

\[VERSION C\]

All wells will be purged prior to sampling. If the well casing volume is
known, a minimum of three casing volumes of water will be purged using
the dedicated well pump, if present, or a bailer, hand pump, or
submersible pump depending on the diameter and configuration of the
well. When a submersible pump is used for purging, clean flexible Teflon
tubes will be used for groundwater extraction. All tubes will be
decontaminated before use in each well. Pumps will be placed 2 to 3 feet
from the bottom of the well to permit reasonable draw down while
preventing cascading conditions.

\[ALL VERSIONS - to be included in all sample plans\]

Water will be collected into a measured bucket to record the purge
volume. Casing volumes will be calculated based on total well depth,
standing water level, and casing diameter. One casing volume will be
calculated as:

**V = d^2^ h / 77.01**

where:

**V** is the volume of one well casing of water (1ft^3^ = 7.48 gallons);

**d** is the inner diameter of the well casing (in inches);

**h** is the total depth of water in the well (in feet).

It is most important to obtain a representative sample from the well.
Stable water quality parameter (temperature, pH and specific
conductance) measurements indicate representative sampling is
obtainable. Water quality is considered stable if for three consecutive
readings:

 temperature range is no more than [+]{.underline}1°C;

 pH varies by no more than 0.2 pH units;

 specific conductance readings are within 10% of the average.

The water in which measurements were taken will not be used to fill
sample bottles.

If the well casing volume is known, measurements will be taken before
the start of purging, in the middle of purging, and at the end of
purging each casing volume. If the well casing volume is NOT known,
measurements will be taken every 2.5 minutes after flow starts. If water
quality parameters are not stable after 5 casing volumes or 30 minutes,
purging will cease, which will be noted in the logbook, and ground water
samples will be taken. The depth to water, water quality measurements
and purge volumes will be entered in the logbook.

If a well dewaters during purging and three casing volumes are not
purged, that well will be allowed to recharge up to 80% of the static
water column and dewatered once more. After water levels have recharged
to 80% of the static water column, groundwater samples will be
collected.

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**6.5.2.3 Well Sampling**

\[Describe the method that will be used to collect samples from wells.
(This will probably be the same method as was used to purge the wells.)
Specify the sequence for sample collection (e.g., bottles for volatile
analysis will be filled first, followed by semivolatiles, etc.). State
whether samples for metals analysis will be filtered or unfiltered.
Include the specific conditions, such as turbidity, that will require
samples to be filtered. Alternatively, reference the appropriate
sections in the Ground Water SOP and state in which Appendix the SOP is
located.\]

ALL VERSIONS - to be included in all sample plans\]

At each sampling location, all bottles designated for a particular
analysis (e.g., volatile organic compounds) will be filled sequentially
before bottles designated for the next analysis are filled (e.g.,
semivolatile organic compounds). If a duplicate sample is to be
collected at this location, all bottles designated for a particular
analysis for both sample designations will be filled sequentially before
bottles for another analysis are filled. In the filling sequence for
duplicate samples, bottles with the two different sample designations
will alternate (e.g., volatile organic compounds designation GW‑2,
volatile organic compounds designation GW‑4 (duplicate of GW‑2), metals
designation GW‑2, metals designation GW‑4 (duplicate of GW‑2).
Groundwater samples will be transferred from the tap directly into the
appropriate sample containers with preservative, if required, chilled if
appropriate, and processed for shipment to the laboratory. When
transferring samples, care will be taken not to touch the tap to the
sample container.

\[If samples are to be collected for volatiles analysis, the following
paragraph should be added; otherwise delete.\]

Samples for volatile organic compound analyses will be collected using a
low flow sampling device. A \[Specify type of pump\] pump will be used
at a flow rate of \_\_\_\_\_\_. Vials for volatile organic compound
analysis will be filled first to minimize the effect of aeration on the
water sample. A test vial will be filled with sample, preserved with
hydrochloric acid (HCl) and tested with pH paper to determine the amount
of preservative needed to lower the pH to less than 2. The appropriate
amount of HCl will then be added to the sample vials prior to the
addition of the sample. The vials will be filled directly from the tap
and capped. The vial will be inverted and checked for air bubbles to
ensure zero headspace. If a bubble appears, the vial will be discarded
and a new sample will be collected.

\[If some samples for metals (or other) analysis are to be filtered,
depending upon sample turbidity, the following paragraph should be
added; otherwise delete.\]

After well purging and prior to collecting groundwater samples for
metals analyses, the turbidity of the groundwater extracted from each
well will be measured using a portable turbidity meter. A small quantity
of groundwater will be collected from the well using the tap and a small
amount of water will be transferred to a disposable vial and a turbidity
measurement will be taken. The results of the turbidity measurement will
be recorded in the field logbook. The water used to measure turbidity
will be discarded after use. If the turbidity of the groundwater from a
well is above 5 Nephelometric Turbidity Units (NTUs), both a filtered
and unfiltered sample will be collected. A \[specify size\]‑micron
filter will be used to remove larger particles that have been entrained
in the water sample. A sample‑dedicated Teflon tube will be attached to
the tap closest to the well head. The filter will be attached to the
outlet of the Teflon tube. A clean, unused filter will be used for each
filtered sample collected. Groundwater samples will be transferred from
the filter directly into the appropriate sample containers with a
preservative and processed for shipment to the laboratory. When
transferring samples, care will be taken not to touch the filter to the
sample container. After the filtered sample has been collected, the
Teflon tube and filter will be removed and an unfiltered sample will be
collected. A sample number appended with an \"Fl\" will represent a
sample filtered with a 5‑micron filter.

\[If samples are to be filtered for metals (or other) analysis
regardless of sample turbidity, the following paragraph should be added;
otherwise delete.\]

Samples designated for metals analysis will be filtered. A 5‑micron
filter will be used to remove larger particles that have been entrained
in the water sample. A sample‑dedicated Teflon tube will be attached to
the tap closest to the well head. The filter will be attached to the
outlet of the Teflon tube. A clean, unused filter will be used for each
filtered sample collected. Groundwater samples will be transferred from
the filter directly into the appropriate sample containers to which
preservative has been added and processed for shipment to the
laboratory. When transferring samples, care will be taken not to touch
the filter to the sample container. After the filtered sample has been
collected, the Teflon tube and filter will be removed and an unfiltered
sample will be collected. A sample number appended with an \"Fl\" will
represent a sample filtered with a 5‑micron filter.

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**6.6 Biological Sampling**

*For the purpose of this guidance, biological sampling falls into two
categories. Other types of biological sampling events should be
discussed with the QA Office to determine what type of planning document
is needed. The two types addressed in this guidance are biological
samples being collected for chemical analysis and biological samples for
the purpose of assessing species diversity. If the latter type of
sampling is planned, a quality assurance project plan may be a more
appropriate document. Samples collected for microbiological analyses
should be discussed under water sampling.*

**6.6.1 Biological Sampling for Chemical Analysis**

\[The two most common types of biological samples being collected for
chemical analysis are fish and foliage samples. The following paragraphs
are suggested, but field circumstances may dictate alternative
collection procedures; if no biological samples will be collected, put
"not applicable" by these sections. If a SOP will be followed, include
it in the appendix.\]

**6.6.1.1 Fish Samples**

\[Use if collecting fish, otherwise delete. Alternatively, reference
appropriate SOPs.\]

Fish will be collected using \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
\[name method; nets, electroshocking, lines, etc.\]. Three fish of each
type or species \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[indicate
type of fish, e. g., trout, catfish, etc.\] will be collected. Efforts
will be made to collect fish of approximately the same size and maturity
by checking to make sure that lengths and weights do not differ by more
than 20%. Once collected the
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ \[indicate
whether whole fish or filets\] will be frozen, wrapped in aluminum foil
and plastic bags and sent to a laboratory designated by the Region 9 QA
Office.

\[If samples are to be composited by the laboratory, also indicate that
in this section.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**6.6.1.2 Foliage Samples**

\[Use if collecting foliage samples, otherwise delete. Alternatively,
reference and include appropriate SOPs. This section may require
considerable modification because of the potential diversity of projects
involving plants sampling.\]

A representative foliage sample will be collected from the target area.
It is recommended that a statistical approach be used, if possible. The
following plants will be collected: \_\_\_\_\_\_\_\_\_\_\_,
\_\_\_\_\_\_\_\_\_\_\_, and \_\_\_\_\_\_\_\_\_\_\_\_\_. These plants are
being collected because they are were most likely affected by chemicals
used in the area. Only foliage showing visible signs of stress or damage
will be collected. Stems and twigs will be discarded; leaves only will
be collected. The same type of leaf material \[Describe material, mature
leaves, young shoots, etc.\] will be obtained from each plant type.
Provided contamination is uniform, material will be composited from
several plants to yield a total of about \[specify quantity\] pound(s)
of material. Control samples will also be collected from a nearby
unaffected area \[Describe area\], if available. Latex gloves will be
worn during the collection of all samples. Samples will be stored in
\[describe container, plastic bags, bottles, etc.\] and brought to the
laboratory as soon as possible to prevent sample deterioration.

**6.6.2 Biological Sampling for Species Assessment**

\[Describe the collection of insects, other invertebrates, or other
types of biological samples here. Reference or attach appropriate
protocols to support the sampling effort\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**6.7 Decontamination Procedures**

\[Specify the decontamination procedures that will be followed if
non-dedicated sampling equipment is used. Alternatively, reference the
appropriate sections in the organization's Decontamination SOP and state
in which Appendix the SOP is located.\]

The decontamination procedures that will be followed are in accordance
with approved procedures. Decontamination of sampling equipment must be
conducted consistently as to assure the quality of samples collected.
All equipment that comes into contact with potentially contaminated soil
or water will be decontaminated. Disposable equipment intended for
one‑time use will not be decontaminated, but will be packaged for
appropriate disposal. Decontamination will occur prior to and after each
use of a piece of equipment. All sampling devices used, including
trowels and augers, will be steam‑cleaned or decontaminated according to
EPA Region 9 recommended procedures.

The following, to be carried out in sequence, is an EPA Region IX
recommended procedure for the decontamination of sampling equipment

\[Use the following decontamination procedure if samples are collected
for organic analyses only; otherwise delete.\]

 Non‑phosphate detergent and tap water wash, using a brush if necessary

 Tap‑water rinse

 Deionized/distilled water rinse

 Pesticide‑grade solvent (reagent grade hexane) rinse in a
decontamination bucket

 Deionized/distilled water rinse (twice)

\[Use the following decontamination procedures if samples are collected
for inorganic (metals) analyses only, otherwise delete.\]

 Non‑phosphate detergent and tap water wash, using a brush if necessary

 Tap‑water rinse

 0.1 N nitric acid rinse

 Deionized/distilled water rinse (twice)

\[Use the following decontamination procedures if samples are collected
for both organic and inorganic analyses, otherwise delete.\]

 Non‑phosphate detergent and tap water wash, using a brush if necessary

 Tap‑water rinse

 0.1 N nitric acid rinse

 Deionized/distilled water rinse

 Pesticide‑grade solvent (reagent grade hexane) rinse in a
decontamination bucket

 Deionized/distilled water rinse (twice)

Equipment will be decontaminated in a predesignated area on pallets or
plastic sheeting, and clean bulky equipment will be stored on plastic
sheeting in uncontaminated areas. Cleaned small equipment will be stored
in plastic bags. Materials to be stored more than a few hours will also
be covered.

\[NOTE: A different decontamination procedure may be used; but if so, a
rationale for using the different approach should be provided.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**7.0 SAMPLE CONTAINERS, PRESERVATION AND STORAGE**

\[This section requires a reference to the types of bottles to be used,
preparation and preservatives to be added. The organization responsible
for adding preservatives should be named. If the information is provided
in the request for analyses tables, reference them in the appropriate
section below.\]

The number of sample containers, volumes, and materials are listed in
Section 5.0. The containers are pre‑cleaned and will not be rinsed prior
to sample collection. Preservatives, if required, will be added by
\_\_\_\_\_\_\_ \[name of agency/organization doing the sampling\] to the
containers prior to shipment of the samples to the laboratory.

**7.1 Soil Samples**

\[If soil samples are to be collected, specify the analyses that will be
performed. Use the language below or reference the appropriate sections
in the Preservation SOP and state in which Appendix the SOP is
located.\]

\[Include this subsection if collecting soil samples; otherwise
delete.\]

\[If requested analyses include analyses other than volatile organic
compounds or metals, include this paragraph; otherwise delete.\]

Soil samples for \_\_\_\_\_\_\_\_\_\_\_\_\_\_\[Include all requested
analysis(es), e.g., Pesticides, Semivolatile Organic Compounds\] will be
homogenized and transferred from the sample‑dedicated homogenization
pail into 8‑ounce (oz), wide‑mouth glass jars using a trowel. For each
sample, one 8-oz wide‑mouth glass jar will be collected for each
laboratory. Alternatively, sample will be retained in the brass sleeve
in which collected until sample preparation begins. The samples will be
chilled to 4°C immediately upon collection.

\[If requested analyses include volatile organic compounds, include this
paragraph; otherwise delete.\]

VOLATILE ORGANIC COMPOUNDS. Soil samples to be analyzed for volatile
organic compounds will be stored in their sealed Encore samplers for no
more than two days prior to analysis. Frozen Encore sampler samples will
be stored for no more than 4 days prior to analysis. If samples are
preserved by ejecting into either methanol or sodium bisulfate solution
the holding time is two weeks. Preserved samples will be chilled to 4°C
immediately upon collection.

\[If requested analyses include metals, include this paragraph;
otherwise delete.\]

METALS. Surface soil samples to be analyzed for metals will be
homogenized and transferred from the sample‑dedicated homogenization
pail into 8-oz, wide‑mouth glass jars. For each sample, one 8-oz glass
jar will be collected for each laboratory. Samples will not be chilled.
Subsurface samples will be retained in their original brass sleeves or
other container unless transferred to bottles.

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**7.2 Sediment Samples**

\[If sediment samples are to be collected, specify the analyses that
will be performed. Use the language below or reference the appropriate
sections in a Preservation SOP and state in which Appendix the SOP is
located.\]

\[If requested analyses include analyses other than volatile organic
compounds or metals, include this paragraph; otherwise delete.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\[Include all requested analysis(es), e.g.,
Pesticides, Semivolatile Organic Compounds\]. Sediment samples will be
homogenized and transferred from the sample‑dedicated homogenization
pail into 8-oz wide‑mouth glass jars. For each sample, one 8-oz glass
jar will be collected for each laboratory. The samples will be chilled
to 4°C immediately upon collection.

\[If requested analyses include volatile organic compounds, include this
paragraph; otherwise delete.\]

VOLATILE ORGANIC COMPOUNDS. Sediment samples to be analyzed for volatile
organic compounds will be stored in their sealed Encore samplers for no
more than two days prior to analysis. Frozen Encore sampler samples will
be stored for no more than 4 days prior to analysis. If samples are
preserved by ejecting into either methanol or sodium bisulfate solution
the holding time is two weeks. Preserved samples will be chilled to 4°C
immediately upon collection.

\[If requested analyses include metals, include this paragraph;
otherwise delete.\]

METALS. Sediment samples, with rocks and debris removed, which are to be
analyzed for metals will be homogenized and transferred from the
sample‑dedicated homogenization pail into 8-oz, wide‑mouth glass jars.
For each sample, one 8-oz glass jar will be collected for each
laboratory. Samples will not be chilled.

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**7.3 Water Samples**

\[If water samples are to be collected, specify the analyses that will
be performed. Use the language below or else reference the appropriate
sections in a Preservation SOP and state in which Appendix the SOP is
located.\]

\[Include this subsection if collecting water samples; otherwise
delete.\]

*Depending on the type of analysis (organic or inorganic) requested, and
any other project‑specific analytical requirements, sample bottles
should be plastic (inorganics) or glass (organics), pre‑cleaned (general
decontamination procedures) or low‑detection level pre‑cleaned
(extensive decontamination procedures).*

\[Describe the type of bottles that will be used for the project,
including the cleaning procedures that will be followed to prepare the
bottles for sampling.\]

\[If requested analyses do not require preservation, include this
paragraph; otherwise delete. A separate paragraph should be included for
each bottle type.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\[Include all requested analysis(es), e.g.,
Anions, Pesticides, Semivolatile Organic Compounds\]. Low concentration
water samples to be analyzed for \_\_\_\_\_\_\_\_\_\_\_\_\_\_\[Specify
analysis(es), e.g., Semivolatile Organic Compounds\] will be collected
in \_\_\_\_\_\_\_\_\_\_\_\_ \[Specify bottle type, e. g., 1‑liter(L)
amber glass bottles\]. No preservative is required for these samples.
The samples will be chilled to 4°C immediately upon collection. Two
bottles of each water sample are required for each laboratory.

\[If requested analyses include volatile organic compounds, include this
paragraph; otherwise delete.\]

VOLATILE ORGANIC COMPOUNDS. Low concentration water samples to be
analyzed for volatile organic compounds will be collected in 40-mL glass
vials. 1:1 hydrochloric acid (HCl) will be added to the vial prior to
sample collection. During purging, the pH will be measured using a pH
meter to test at least one vial at each sample location to ensure
sufficient acid is present to result in a pH of less than 2. The tested
vial will be discarded. If the pH is greater than 2, additional HCl will
be added to the sample vials. Another vial will be pH tested to ensure
the pH is less than 2. The tested vial will be discarded. The vials will
be filled so that there is no headspace. The samples will be chilled to
4°C immediately upon collection. Three vials of each water sample are
required for each laboratory.

\[If requested analyses include metals, include this paragraph;
otherwise delete.\]

METALS. Water samples collected for metals analysis will be collected in
1L polyethylene bottles. The samples will be preserved by adding nitric
acid (HNO~3~) to the sample bottle. The bottle will be capped and
lightly shaken to mix in the acid. A small quantity of sample will be
poured into the bottle cap where the pH will be measured using pH paper.
The pH must be [\<]{.underline}2. The sample in the cap will be
discarded, and the pH of the sample will be adjusted further if
necessary. The samples will be chilled to 4°C immediately upon
collection. One bottle of each water sample is required for each
laboratory.

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

GENERAL CHEMISTRY (WATER QUALITY) PARAMETERS. Water samples collected
for water quality analysis \[Specify what parameters are included.
Examples include (but are not limited to) anions (nitrate-N, nitrite-N,
sulfate, phosphate), total phosphorus, ammonia-N, total dissolved
solids, total suspended solids, alkalinity (may include carbonate,
and/or bicarbonate), hardness, cyanide, MBAS (methylene blue active
substances), etc.\], will be collected in \[Specify size of container\]
polyethylene bottles. The \[Specify analysis\] samples will be preserved
by adding \[Describe preservative appropriate to each sample type\] to
the sample bottle. The \[Specify analysis\] samples will not be
preserved. If preservative is added, the bottle will be capped and
lightly shaken to mix in the preservative. Where the preservative
affects the pH, a small quantity of sample will be poured into the
bottle cap where the pH will be measured using pH paper. The pH must be
within the appropriate range. The sample in the cap will be discarded,
and the pH of the sample will be adjusted further if necessary. Samples
will be chilled to 4°C immediately upon collection. Samples from each
location that require the same preservative will be placed in the same
bottle if being analyzed by the same laboratory.

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**7.4 Biological Samples**

\[If biological samples are to be collected, specify the analyses that
will be performed. Use the language below or reference the appropriate
sections in a Preservation SOP and state in which Appendix the SOP is
located.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**7.4.1 Fish Samples**

Fish (whole or fillets) will be wrapped in aluminum foil, labeled, and
placed in individual zip lock bags. The samples will be frozen as
quickly as possible and shipped using dry ice to maintain the frozen
state.

**7.4.2 Foliage Samples**

\[Describe the containers that will be used for the project. Usually
foliage samples are collected in clean zip lock bags, but bottles or
other containers can be used. Paper bags are not recommended.\]

For foliage samples, samples will be collected in one gallon zip lock. A
self adhesive label will be placed on each bag and the top sealed with a
custody seal.

**7.4.3 Biological Sampling for Species Assessment**

\[Describe the containers in which macroinvertebrates, insects and other
biological samples will be stored. If a fixation liquid will be used, it
should be described as well. This section should also discuss any
special handling procedures which must be followed to minimize damage to
the specimens.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**8.0 DISPOSAL OF RESIDUAL MATERIALS**

\[This section should describe the type(s) of investigation‑ derived
wastes (IDW) that will be generated during this sampling event. EPA
recognizes that IDW may not be generated in all sampling events, in
which case this section would not apply. Use the language below or
reference the appropriate sections in a Disposal of Residual Materials
SOP and state in which Appendix the SOP is located. Depending upon
site‑specific conditions and applicable federal, state, and local
regulations, other provisions for IDW disposal may be required. If any
analyses of IDW are required, these should be discussed. If IDW are to
be placed in drums, labeling for the drums should be discussed in this
section.\]

In the process of collecting environmental samples at the
\_\_\_\_\_\_\_\_\_\[site or sampling area name\] during the site
investigation (SI) \[or name of other investigation\], the
\_\_\_\_\_\_\_\_\[name of your organization/agency\] sampling team will
generate different types of potentially contaminated IDW that include
the following:

 Used personal protective equipment (PPE)

 Disposable sampling equipment

 Decontamination fluids

\[Include this bullet when sampling soils; otherwise delete.\]

 Soil cuttings from soil borings

\[Include this bullet when sampling groundwater; otherwise delete.\]

 Purged groundwater and excess groundwater collected for sample
container filling.

The EPA\'s National Contingency Plan (NCP) requires that management of
IDW generated during sampling comply with all applicable or relevant and
appropriate requirements (ARARs) to the extent practicable. The sampling
plan will follow the *Office of Emergency and Remedial Response (OERR)
Directive 9345.3‑02* (May 1991), which provides the guidance for the
management of IDW. In addition, other legal and practical considerations
that may affect the handling of IDW will be considered.

\[Listed below are the procedures that should be followed for handling
the IDW. The procedures have enough flexibility to allow the sampling
team to use its professional judgment as to the proper method for the
disposal of each type of IDW generated at each sampling location.\]

\[The following bullet is generally appropriate for site or sampling
areas with low levels of contamination or for routine monitoring. If
higher levels of contamination exist at the site or sampling area, other
disposal methods (such as the drumming of wastes) should be used to
dispose of used PPE and disposable sampling equipment.\]

 Used PPE and disposable equipment will be double bagged and placed in
a municipal refuse dumpster. These wastes are not considered hazardous
and can be sent to a municipal landfill. Any PPE and disposable
equipment that is to be disposed of which can still be reused will be
rendered inoperable before disposal in the refuse dumpster.

\[Include this bullet if sampling for both metals and organics;
otherwise delete.\]

 Decontamination fluids that will be generated in the sampling event
will consist of dilute nitric acid, pesticide‑grade solvent, deionized
water, residual contaminants, and water with non‑phosphate detergent.
The volume and concentration of the decontamination fluid will be
sufficiently low to allow disposal at the site or sampling area. The
water (and water with detergent) will be poured onto the ground or into
a storm drain. Pesticide‑grade solvents will be allowed to evaporate
from the decontamination bucket. The nitric acid will be diluted and/or
neutralized with sodium hydroxide and tested with pH paper before
pouring onto the ground or into a storm drain.

\[Include this bullet if sampling for metals but not organics; otherwise
delete.\]

 Decontamination fluids that will be generated in the sampling event
will consist of nitric acid, deionized water, residual contaminants, and
water with non‑phosphate detergent. The volume and concentration of the
decontamination fluid will be sufficiently low to allow disposal at the
site or sampling area. The water (and water with detergent) will be
poured onto the ground or into a storm drain. The nitric acid will be
diluted and/or neutralized with sodium hydroxide and tested with pH
paper before pouring onto the ground or into a storm drain.

\[Include this bullet if sampling for organics but not metals; otherwise
delete.\]

 Decontamination fluids that will be generated in the sampling event
will consist of pesticide‑grade solvent, deionized water, residual
contaminants, and water with non‑phosphate detergent. The volume and
concentration of the decontamination fluid will be sufficiently low to
allow disposal at the site or sampling area. The water (and water with
detergent) will be poured onto the ground or into a storm drain.
Pesticide‑grade solvents will be allowed to evaporate from the
decontamination bucket.

\[Include this bullet if sampling soils; otherwise delete.\]

 Soil cuttings generated during the subsurface sampling will be
disposed of in an appropriate manner.

\[Include this bullet if sampling groundwater; otherwise delete.\]

 Purged groundwater will be \_\_\_\_\_\_\_\_\_\_\_\_\_\_

\[Depending upon the degree of groundwater contamination, site‑specific
conditions, and applicable federal, state, and local regulations,
disposal methods will vary. Disposal methods can also vary for purge
water from different wells sampled during the same sampling event\].

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**9.0 SAMPLE DOCUMENTATION AND SHIPMENT**

**9.1 Field Notes**

*This section should discuss record keeping in the field. This may be
through a combination of logbooks, preprinted forms, photographs, or
other documenttion. Information to be maintained is provided below.*

**9.1.1 Field Logbooks**

\[Describe how field logbooks will be used and maintained.\]

*Use field logbooks to document where, when, how, and from whom any
vital project information was obtained. Logbook entries should be
complete and accurate enough to permit reconstruction of field
activities. Maintain a separate logbook for each sampling event or
project. Logbooks should have consecutively numbered pages. All entries
should be legible, written in black ink, and signed by the individual
making the entries. Use factual, objective language.*

At a minimum, the following information will be recorded during the
collection of each sample:

\[Edit this list as relevant.\]

 Sample location and description

 Site or sampling area sketch showing sample location and measured
distances

 Sampler\'s name(s)

 Date and time of sample collection

 Designation of sample as composite or grab

 Type of sample (soil, sediment or water)

 Type of sampling equipment used

 Field instrument readings and calibration

 Field observations and details related to analysis or integrity of
samples (e.g., weather conditions, noticeable odors, colors, etc.)

 Preliminary sample descriptions (e.g., for soils: clay loam, very wet;
for water: clear water with strong ammonia‑like odor)

 Sample preservation

 Lot numbers of the sample containers, sample identification numbers
and any explanatory codes, and chain‑of‑custody form numbers

 Shipping arrangements (overnight air bill number)

 Name(s) of recipient laboratory(ies)

In addition to the sampling information, the following specific
information will also be recorded in the field logbook for each day of
sampling:

\[Edit this list as relevant.\]

 Team members and their responsibilities

 Time of arrival/entry on site and time of site departure

 Other personnel on site

 Summary of any meetings or discussions with tribal, contractor, or
federal agency personnel

 Deviations from sampling plans, site safety plans, and QAPP procedures

 Changes in personnel and responsibilities with reasons for the changes

 Levels of safety protection

 Calibration readings for any equipment used and equipment model and
serial number

\[A checklist of the field notes, following the suggestions above, using
only those that are appropriate, should be developed and included in
project field notes.\]

**9.1.2 Photographs**

\[If photographs will be taken, the following language may be used as is
or modified as appropriate.\]

Photographs will be taken at the sampling locations and at other areas
of interest on site or sampling area. They will serve to verify
information entered in the field logbook. For each photograph taken, the
following information will be written in the logbook or recorded in a
separate field photography log:

 Time, date, location, and weather conditions

 Description of the subject photographed

 Name of person taking the photograph

**9.2 Labeling**

\[The following paragraph provides a generic explanation and description
of the use of labels. It may be incorporated as is, if appropriate, or
modified to meet any project‑specific conditions.\]

All samples collected will be labeled in a clear and precise way for
proper identification in the field and for tracking in the laboratory. A
copy of the sample label is included in Appendix \_\_. The samples will
have preassigned, identifiable, and unique numbers. At a minimum, the
sample labels will contain the following information: station location,
date of collection, analytical parameter(s), and method of preservation.
Every sample, including samples collected from a single location but
going to separate laboratories, will be assigned a unique sample number.

**9.3 Sample Chain‑Of‑Custody Forms and Custody Seals**

\[The following paragraphs provide a generic explanation and description
of the use of chain‑of‑custody forms and custody seals. They may be
incorporated as is, if they are appropriate, or modified to meet any
project‑specific conditions.\]

\[If Contract Laboratory Program Analytical Services (CLPAS)
laboratories are to be used, include the following sentence; otherwise
delete.\]

Organic and inorganic chain‑of‑custody record/traffic report forms are
used to document sample collection and shipment to laboratories for
analysis.

\[If Regional Analytical Program (RAP) laboratories are to be used
(either the Regional laboratory in Richmond, CA, or other Region 9
scheduled commercial laboratories) include the following sentence;
otherwise delete.\]

Regional Analytical Program (RAP) chain‑of‑custody record forms are used
to document sample collection and shipment to laboratories for analysis.

All sample shipments for analyses will be accompanied by a
chain‑of‑custody record. A copy of the form is found in Appendix \_.
Form(s) will be completed and sent with the samples for each laboratory
and each shipment (i.e., each day). Proper distribution of the forms is
found in the \"Instructions for Sample Shipping and Documentation\"
guidance document. If multiple coolers are sent to a single laboratory
on a single day, form(s) will be completed and sent with the samples for
each cooler.

The chain‑of‑custody form will identify the contents of each shipment
and maintain the custodial integrity of the samples. Generally, a sample
is considered to be in someone\'s custody if it is either in someone\'s
physical possession, in someone\'s view, locked up, or kept in a secured
area that is restricted to authorized personnel. Until the samples are
shipped, the custody of the samples will be the responsibility of
\_\_\_\_\_\[name of agency/ organization conducting sampling\]. The
sampling team leader or designee will sign the chain‑of‑custody form in
the \"relinquished by\" box and note date, time, and air bill number.

The sample numbers for all rinsate samples, reference samples,
laboratory QC samples, and duplicates will be documented on this form
(see Section 10.0). The original form will be sent to the Regional
Sample Control Coordinator (RSCC) in the QA Office; a photocopy will be
made for the \_\_\_\_\_\[name of agency/ organization conducting
sampling\] master files. This form is not sent to the laboratory, but is
sent to the QA Office when samples are shipped.

A self‑adhesive custody seal will be placed across the lid of each
sample. A copy of the seal is found in Appendix \_. For VOC samples, the
seal will be wrapped around the cap. The shipping containers in which
samples are stored (usually a sturdy picnic cooler or ice chest) will be
sealed with self‑adhesive custody seals any time they are not in
someone\'s possession or view before shipping. All custody seals will be
signed and dated.

**9.4 Packaging and Shipment**

\[The following paragraphs provide a generic explanation and description
of how to pack and ship samples. They may be incorporated as is, if
appropriate, or modified to meet any project‑specific conditions.\]

All sample containers will be placed in a strong‑outside shipping
container (a steel‑belted cooler). The following outlines the packaging
procedures that will be followed for low concentration samples.

1\. When ice is used, pack it in zip‑locked, double plastic bags. Seal
the drain plug of the cooler with fiberglass tape to prevent melting ice
from leaking out of the cooler.

2\. The bottom of the cooler should be lined with bubble wrap to prevent
breakage during shipment.

3\. Check screw caps for tightness and, if not full, mark the sample
volume level of liquid samples on the outside of the sample bottles with
indelible ink.

4\. Secure bottle/container tops with clear tape and custody seal all
container tops.

5\. Affix sample labels onto the containers with clear tape.

6\. Wrap all glass sample containers in bubble wrap to prevent breakage.

7\. Seal all sample containers in heavy duty plastic zip-lock bags.
Write the sample numbers on the outside of the plastic bags with
indelible ink.

8\. Place samples in a sturdy cooler(s) lined with a large plastic trash
bag. Enclose the appropriate COC(s) in a zip-lock plastic bag affixed to
the underside of the cooler lid.

9\. Fill empty space in the cooler with bubble wrap or Styrofoam peanuts
to prevent movement and breakage during shipment.

10\. Ice used to cool samples will be double sealed in two zip lock
plastic bags and placed on top and around the samples to chill them to
the correct temperature.

11\. Each ice chest will be securely taped shut with fiberglass
strapping tape, and custody seals will be affixed to the front, right
and back of each cooler.

The EPA Region 9 Regional Sample Control Center (RSCC: 415‑744‑1498)
will be notified daily of the sample shipment schedule (Friday shipments
must be reported no later than noon) and will be provided with the
following information:

 Sampling contractor\'s name

 Name and location of the site or sampling area

 Case or Regional Analytical Program (RAP) number

 Total number(s) by concentration and matrix of samples shipped to each
laboratory

 Carrier, air bill number(s), method of shipment (priority next day)

 Shipment date and when it should be received by lab

 Irregularities or anticipated problems associated with the samples

 Whether additional samples will be shipped or if this is the last
shipment.

**10.0 QUALITY CONTROL**

*This section should discuss the quality control samples that are being
collected to support the sampling activity. This includes field QC
samples, confirmation samples, background samples, laboratory QC
samples, and split samples. Wherever possible, the locations at which
the samples will be collected should be identified and a rationale
provided for the choice of location. Frequency of collection should be
discussed. All samples, except laboratory QC samples, should be sent to
the laboratory blind, wherever possible. Laboratory QC samples should be
identified and additional sample (e.g., a double volume) collected for
that purpose.*

**10.1 Field Quality Control Samples**

*Field quality control samples are intended to help evaluate conditions
resulting from field activities and are intended to accomplish two
primary goals, assessment of field contamination and assessment of
sampling variability. The former looks for substances introduced in the
field due to environmental or sampling equipment and is assessed using
blanks of different types. The latter includes variability due to
sampling technique and instrument performance as well as variability
possibly caused by the heterogeneity of the matrix being sampled and is
assessed using replicate sample collection. The following sections cover
field QC.*

**10.1.1** **Assessment of Field Contamination (Blanks)**

*Field contamination is usually assessed through the collection of
different types of blanks. Equipment blanks are obtained by passing
distilled or deionized water, as appropriate, over or through the
decontaminated equipment used for sampling. They provide the best
overall means of assessing contamination arising from the equipment,
ambient conditions, sample containers, transit, and the laboratory.
Field blanks are sample containers filled in the field. They help assess
contamination from ambient conditions, sample containers, transit, and
the laboratory. Trip blanks are prepared by the laboratory and shipped
to and from the field. They help assess contamination from shipping and
the laboratory and are for volatile organic compounds only. Region 9
recommends that equipment blanks be collected, where appropriate (e.g.,
where neither disposable or dedicated equipment is used). Field blanks
are next in priority, and trip blanks next. Only one type of blank must
be collected per event, not all three.*

**10.1.1.1 Equipment Blanks**

*In general, equipment (rinsate) blanks should be collected when
reusable, non‑disposable sampling equipment (e.g., trowels, hand augers,
and non-dedicated groundwater sampling pumps) are being used for the
sampling event. Only one blank sample per matrix per day should be
collected. If equipment blanks are collected, field blanks and trip
blanks are not required under normal circumstances. Equipment blanks can
be collected for soil, sediment, and ground water samples. A minimum of
one equipment blank is prepared each day for each matrix when equipment
is decontaminated in the field. These blanks are submitted \"blind\" to
the laboratory, packaged like other samples and each with its own unique
identification number. Note that for samples which may contain VOCs,
water for blanks should be purged prior to use to ensure that it is
organic free. HPLC water which is often used for equipment and field
blanks, can contain VOCs if it is not purged.*

\[If equipment blanks are to be collected describe how they are to be
collected and the analyses that will be performed. A maximum of one
blank sample per matrix per day should be collected, but at a rate to
not exceed one blank per 10 samples. The 1:10 ratio overrides the one
per day requirement. If equipment rinsate blanks are collected, field
blanks and trip blanks are not required under normal circumstances. Use
the language below or reference the appropriate sections in a Quality
Control SOP and state in which Appendix the SOP is located.\]

\[Include this subsection if equipment blanks are to be collected,
otherwise, delete.\]

\[Include this paragraph if blanks will be analyzed for both metals and
organic compounds; otherwise delete.\]

Equipment rinsate blanks will be collected to evaluate field sampling
and decontamination procedures by pouring High Performance Liquid
Chromatography (HPLC) organic‑free (for organics) or deionized water
(for inorganics) over the decontaminated sampling equipment. One
equipment rinsate blank will be collected per matrix each day that
sampling equipment is decontaminated in the field. Equipment rinsate
blanks will be obtained by passing water through or over the
decontaminated sampling devices used that day. The rinsate blanks that
are collected will be analyzed for \_\_\_\_\_\_\_\_

\[Include names of target analytes, e.g., metals, total petroleum
hydrocarbons, volatile organic compounds, etc.\].

\[Include this paragraph if blanks will be analyzed only for organic
compounds; otherwise delete.\]

Equipment rinsate blanks will be collected to evaluate field sampling
and decontamination procedures by pouring High Performance Liquid
Chromatography (HPLC) organic‑free water over the decontaminated
sampling equipment. One equipment rinsate blank will be collected per
matrix each day that sampling equipment is decontaminated in the field.
Equipment rinsate blanks will be obtained by passing water through or
over the decontaminated sampling devices used that day. The rinsate
blanks that are collected will be analyzed for \_\_\_\_\_\_\_\_\_

\[Include names of target analytes, e.g., volatile organic compounds,
total petroleum hydrocarbons, etc.\]

\[Include this paragraph if blanks will be analyzed only for metals;
otherwise delete.\]

Equipment rinsate blanks will be collected to evaluate field sampling
and decontamination procedures by pouring deionized water over the
decontaminated sampling equipment. One equipment rinsate blank will be
collected per matrix each day that sampling equipment is decontaminated
in the field. Equipment rinsate blanks will be obtained by passing
deionized water through or over the decontaminated sampling devices used
that day. The rinsate blanks that are collected will be analyzed for
metals.

\[Always include this paragraph.\]

The equipment rinsate blanks will be preserved, packaged, and sealed in
the manner described for the environmental samples. A separate sample
number and station number will be assigned to each sample, and it will
be submitted blind to the laboratory.

**10.1.1.2 Field Blanks**

*Field blanks are collected when sampling water or air and equipment
decontamination is not necessary or sample collection equipment is not
used (e.g., dedicated pumps). A minimum of one field blank is prepared
each day sampling occurs in the field, but equipment is not
decontaminated. These blanks are submitted \"blind\" to the laboratory,
packaged like other samples and each with its own unique identification
number. Note that for samples which may contain VOCs, water for blanks
should be purged prior to use to ensure that it is organic free. HPLC
water which is often used for equipment and field blanks, can contain
VOCs if it is not purged.*

\[Include this subsection if field blanks will be collected; otherwise
delete. Only one blank sample per matrix per day should be collected. If
field blanks are prepared, equipment rinsate blanks and trip blanks are
not required under normal circumstances.\]

\[Include this paragraph if blanks will be analyzed for both metals and
organic compounds; otherwise delete.\]

Field blanks will be collected to evaluate whether contaminants have
been introduced into the samples during the sampling due to ambient
conditions or from sample containers. Field blank samples will be
obtained by pouring High Performance Liquid Chromatography (HPLC)
organic‑free water (for organics) and/or deionized water (for
inorganics) into a sampling container at the sampling point. The field
blanks that are collected will be analyzed for \_\_\_\_\_\_\_\_\_

\[Include names of target analytes, e.g., metals, volatile organic
compounds, etc.\].

\[Include this paragraph if blanks will be analyzed only for organic
compounds; otherwise delete.\]

Field blanks will be collected to evaluate whether contaminants have
been introduced into the samples during the sampling due to ambient
conditions or from sample containers. Field blank samples will be
obtained by pouring High Performance Liquid Chromatography (HPLC)
organic‑free water into a sampling container at the sampling point. The
field blanks that are collected will be analyzed for \_\_\_\_\_\_\_\_\_

\[Include names of target analytes, e.g., volatile organic compounds,
total petroleum hydrocarbons, etc.\].

\[Include this paragraph if blanks will be analyzed only for metals;
otherwise delete.\]

Field blanks will be collected to evaluate whether contaminants have
been introduced into the samples during the sampling due to
contamination from sample containers. Field blank samples will be
obtained by pouring deionized water into a sampling container at the
sampling point. The field blanks that are collected will be analyzed for
metals.

\[Always include this paragraph.\]

The field blanks will be preserved, packaged, and sealed in the manner
described for the environmental samples. A separate sample number and
station number will be assigned to each sample, and it will be submitted
blind to the laboratory.

**10.1.1.3 Trip Blanks**

*Trip blanks are required only if no other type of blank will be
collected for volatile organic compound analysis and when air and/or
water samples are being collected. If trip blanks are required, one is
submitted to the laboratory for analysis with every shipment of samples
for VOC analysis. These blanks are submitted \"blind\" to the
laboratory, packaged like other samples and each with its own unique
identification number. Note that for samples which may contain VOCs,
water for blanks should be purged prior to use to ensure that it is
organic free. Laboratory water which is used for trip blanks, can
contain VOCs if it is not purged.*

\[Include this subsection if trip blanks will be collected; otherwise
delete. Only one blank sample per matrix per day should be collected.
Trip blanks are only relevant to volatile organic compound (VOC)
sampling efforts.\]

Trip blanks will be prepared to evaluate if the shipping and handling
procedures are introducing contaminants into the samples, and if cross
contamination in the form of VOC migration has occurred between the
collected samples. A minimum of one trip blank will be submitted to the
laboratory for analysis with every shipment of samples for VOC analysis.
Trip blanks are 40-mL vials that have been filled with HPLC‑grade water
that has been purged so it is VOC free and shipped with the empty
sampling containers to the site or sampling area prior to sampling. The
sealed trip blanks are not opened in the field and are shipped to the
laboratory in the same cooler with the samples collected for volatile
analyses. The trip blanks will be preserved, packaged, and sealed in the
manner described for the environmental samples. A separate sample number
and station number will be assigned to each trip sample and it will be
submitted blind to the laboratory.

**10.1.1.4 Temperature Blanks**

\[Include this paragraph with all plans\]

For each cooler that is shipped or transported to an analytical
laboratory a 40 mL VOA vial will be included that is marked "temperature
blank." This blank will be used by the sample custodian to check the
temperature of samples upon receipt.

**10.1.2 Assessment of Sample Variability (Field Duplicate or Co-located
Samples)**

*Duplicate samples are collected simultaneously with a standard sample
from the same source under identical conditions into separate sample
containers. Field duplicates will consist of a homogenized sample
divided in two or else a co-located sample. Each duplicate portion
should be assigned its own sample number so that it will be blind to the
laboratory. A duplicate sample is treated independently of its
counterpart in order to assess laboratory performance through comparison
of the results. At least 10% of samples collected per event should be
field duplicates. At least one duplicate should be collected for each
sample matrix, but their collection can be stretched out over more than
one day (e.g., if it takes more than one day to reach 10 samples). Every
group of analytes for which a standard sample is analyzed will also be
tested for in one or more duplicate samples. Duplicate samples should be
collected from areas of known or suspected contamination.* *Since the
objective is to assess variability due to sampling technique and
possible sample heterogeneity, source variability is a good reason to
collect co-located samples, not to avoid their collection.*

\[Include this paragraph if collecting soil samples; otherwise delete.\]

Duplicate soils samples will be collected at sample locations
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\[Identify soil sample locations from which duplicate or collocated
samples will be collected for duplicate analysis will be obtained\].

Duplicate samples will be collected from these locations because
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\[Add sentence(s) here explaining a rationale for collecting duplicate
samples from these locations; e.g., samples from these locations are
suspected to exhibit moderate concentrations of contaminants or previous
sampling events have detected moderate levels of contamination at the
site or sampling area at these locations.\]

\[Include this paragraph if collecting soil samples and analyzing for
compounds other than volatiles; otherwise delete.\]

Soil samples to be analyzed for
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\[List all analytical methods for
this sample event except for volatiles\] will be homogenized with a
trowel in a sample‑dedicated 1‑gallon disposable pail. Homogenized
material from the bucket will then be transferred to the appropriate
wide‑mouth glass jars for both the regular and duplicate samples. All
jars designated for a particular analysis (e.g., semivolatile organic
compounds) will be filled sequentially before jars designated for
another analysis are filled (e.g., metals).

\[Include this paragraph if collecting soil samples and analyzing for
volatiles; otherwise delete.\]

Soil samples for volatile organic compound analyses will not be
homogenized. Equivalent Encore samples from a collocated location will
be collected identically to the original samples, assigned unique sample
numbers and sent blind to the laboratory.

\[Include these paragraphs if collecting sediment samples. If volatile
organic compound analysis will be performed on sediment samples, modify
the above paragraph for soil sample volatile analyses by changing
\"soil\" to \"sediment\".\]

Duplicate sediment samples will be collected at sample locations
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_.

\[Identify sediment sample locations from which duplicate or collocated
samples for duplicate analysis will be obtained\].

Duplicate samples will be collected from these locations because
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_.

\[Add sentence(s) here explaining a rationale for collecting duplicate
samples from these locations; e.g., samples from these locations are
suspected to exhibit moderate concentrations of contaminants or previous
sampling events have detected moderate levels of contamination at the
site or sampling area at these locations.\]

Sediment samples will be homogenized with a trowel in a sample‑dedicated
1‑gallon disposable pail. Homogenized material from the bucket will then
be transferred to the appropriate wide‑mouth glass jars for both the
regular and duplicate samples. All jars designated for a particular
analysis (e.g., semivolatile organic compounds) will be filled
sequentially before jars designated for another analysis are filled
(e.g., metals).

\[Include this paragraph if collecting water samples.\]

Duplicate water samples will be collected for water sample numbers
\_\_\_\_\_\_\_\_\_\_\_\_\_.

\[Water sample numbers which will be split for duplicate analysis\].

Duplicate samples will be collected from these locations because
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_.

\[Add sentence(s) here explaining a rationale for collecting duplicate
samples from these locations; e.g. samples from these locations are
suspected to exhibit moderate concentrations of contaminants or previous
sampling events have detected moderate levels of contamination at the
site or sampling area at these locations.\] When collecting duplicate
water samples, bottles with the two different sample identification
numbers will alternate in the filling sequence (e.g., a typical filling
sequence might be, VOCs designation GW‑2, VOCs designation GW‑4
(duplicate of GW‑2); metals, designation GW‑2, metals, designation GW‑4,
(duplicate of GW‑2) etc.). Note that bottles for one type of analysis
will be filled before bottles for the next analysis are filled.
Volatiles will always be filled first.

\[Always include this paragraph.\]

Duplicate samples will be preserved, packaged, and sealed in the same
manner as other samples of the same matrix. A separate sample number and
station number will be assigned to each duplicate, and it will be
submitted blind to the laboratory.

**10.2 Background Samples**

*Background samples are collected in situations where the possibility
exists that there are native or ambient levels of one or more target
analytes present or where one aim of the sampling event is to
differentiate between on-site and off-site contributions to
contamination. One or more locations are chosen which should be free of
contamination from the site or sampling location itself, but have
similar geology, hydrogeology, or other characteristics to the proposed
sampling locations that may have been impacted by site activities. For
example, an area adjacent to but removed from the site, upstream from
the sampling points, or up gradient or cross gradient from the
groundwater under the site. Not all sampling events require background
samples.*

\[Specify the sample locations that have been designated as background.
Include a rationale for collecting background samples from these
locations and describe or reference the sampling and analytical
procedures which will be followed to collect these samples.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**10.3 Field Screening, Confirmation, and Split Samples**

*For projects where field screening methods are used (typically defined
as testing using field test kits, immunoassay kits, or soil gas
measurements, or equivalent, but not usually defined as the use of a
mobile laboratory which generates data equivalent to a fixed
laboratory), two aspects of the tests should be described. First, the QC
which will be run in conjunction with the field screening method itself,
and, second, any fixed laboratory confirmation tests which will be
conducted. QC acceptance criteria for these tests should be defined in
these sections rather than in the DQO section.*

**10.3.1 Field Screening Samples**

\[For projects where field screening methods are used describe the QC
samples which will be run in the field to ensure that the screening
method is working properly. This usually consists of a combination of
field duplicates and background (clean) samples). The discussion should
specify acceptance criteria and corrective action to be taken if results
are not within defined limits. Discuss confirmation tests below.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**10.3.2 Confirmation Samples**

*If the planned sampling event includes a combination of field screening
and fixed laboratory confirmation, this section should describe the
frequency with which the confirmation samples will be collected and the
criteria which will be used to select confirmation locations. These will
both be dependent on the use of the data in decision making. It is
recommended that the selection process be at a minimum of 10% and that a
selection criteria include checks for both false positives (i.e., the
field detections are invalid or the concentrations are not accurate) and
false negatives (i.e., the analyte was not detected in the field).
Because many field screening techniques are less sensitive than
laboratory methods false negative screening is especially important
unless the field method is below the action level for any decision
making. It is recommended that some "hits" be chosen and that other
locations be chosen randomly.*

\[Describe confirmation sampling. Discuss the frequency with which
samples will be confirmed and how location will be chosen. Define
acceptance criteria for the confirmation results (e.g., RPD25%) and
corrective actions to be taken if samples are not confirmed.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**10.3.3 Split Samples**

*Split Samples are defined differently by different organizations, but
for the purpose of this guidance, Region 9\'s QA Office considers split
samples as ones that are divided among two or more laboratory for the
purpose of providing an inter-laboratory or inter-organization
comparison. Usually one organization (for example, a responsible party)
collects the samples and provides sufficient material to the other
organization (for example, EPA) to enable it to perform independent
analyses. It is expected that the sampling party will have prepared a
sampling plan which the QA Office has reviewed and approved that
describes the sampling locations and a rationale for their choice,
sampling methods, and analyses.*

\[Describe the purpose of the split sampling. Include references to the
approved sampling plan of the party collecting the samples. Provide a
rationale for the sample locations at which split samples will be
obtained and how these locations are representative of the sampling
event as a whole. Describe how results are to be compared and define
criteria by which agreement will be measured. Discuss corrective action
to be taken if results are found to not be in agreement.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**10.4 Laboratory Quality Control Samples**

*Laboratory quality control (QC) samples are analyzed as part of
standard laboratory practice. The laboratory monitors the precision and
accuracy of the results of its analytical procedures through analysis of
QC samples. In part, laboratory QC samples consist of matrix
spike/matrix spike duplicate samples for organic analyses, and matrix
spike and duplicate samples for inorganic analyses. The term \"matrix\"
refers to use of the actual media collected in the field (e.g., routine
soil and water samples).*

*Laboratory QC samples are an aliquot (subset) of the field sample. They
are not a separate sample, but a special designation of an existing
sample.*

\[Include the following language if soil samples are to be collected for
other than VOCs. Otherwise delete.\]

A routinely collected soil sample (a full 8-oz sample jar or two 120-mL
sample vials) contains sufficient volume for both routine sample
analysis and additional laboratory QC analyses. Therefore, a separate
soil sample for laboratory QC purposes will not be collected.

\[Include the following language if soil samples are to be collected for
other than VOCs. Otherwise delete.\]

Soil samples for volatile organic compound analyses for laboratory QC
purposes will be obtained by collecting double the number of equivalent
Encore samples from a collocated location in the same way as the
original samples, assigned a unique sample numbers and sent blind to the
laboratory.

\[Include the following language if water samples are to be collected.
Otherwise delete.\]

For water samples, double volumes of samples are supplied to the
laboratory for its use for QC purposes. Two sets of water sample
containers are filled and all containers are labeled with a single
sample number.

*For VOC samples this would result in 6 vials being collected instead of
3, for pesticides and semivolatile samples this would be 4 liters
instead of 2, etc.\]*

The laboratory should be alerted as to which sample is to be used for QC
analysis by a notation on the sample container label and the
chain‑of‑custody record or packing list.

At a minimum, one laboratory QC sample is required per 14 days or one
per 20 samples (including blanks and duplicates), whichever is greater.
If the sample event lasts longer than 14 days or involves collection of
more than 20 samples per matrix, additional QC samples will be
designated.

For this sampling event, samples collected at the following locations
will be the designated laboratory QC samples:

\[If a matrix is not being sampled, delete the reference to that
matrix.\]

 For soil, samples \_\_\_\_\_\_\_\_\_\_\_\_

\[List soil sample locations and numbers designated for QA/QC\]

 For sediment, samples \_\_\_\_\_\_\_\_\_\_\_\_,

\[List sediment sample locations and numbers designated for QA/QC\]

 For water, samples \_\_\_\_\_\_\_\_\_\_\_\_.

\[List water sample locations and numbers designated for QA/QC\]

\[Add a paragraph explaining why these sample locations were chosen for
QA/QC samples. QA/QC samples should be samples expected to contain
moderate levels of contamination. A rationale should justify the
selection of QA/QC samples based on previously‑detected contamination at
the site or sampling area, historic site or sampling area operations,
expected contaminant deposition/migration, etc.\]

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

**11.0 FIELD VARIANCES**

\[It is not uncommon to find that, on the actual sampling date,
conditions are different from expectations such that changes must be
made to the SAP once the samplers are in the field. The following
paragraph provides a means for documenting those deviations, or
variances. Adopt the paragraph as is, or modify it to project‑specific
conditions.\]

As conditions in the field may vary, it may become necessary to
implement minor modifications to sampling as presented in this plan.
When appropriate, the QA Office will be notified and a verbal approval
will be obtained before implementing the changes. Modifications to the
approved plan will be documented in the sampling project report.

**12.0 FIELD HEALTH AND SAFETY PROCEDURES**

\[Describe any agency‑, program‑ or project‑specific health and safety
procedures that must be followed in the field, including safety
equipment and clothing that may be required, explanation of potential
hazards that may be encountered, and location and route to the nearest
hospital or medical treatment facility. A copy of the organization
health and safety plan may be included in the Appendix and referenced in
this section.\]

**Table 5-1**

**REQUEST FOR ANALYTICAL SERVICES**

**MATRIX = SOIL**

<table style="width:100%;">
<colgroup>
<col style="width: 7%" />
<col style="width: 8%" />
<col style="width: 5%" />
<col style="width: 7%" />
<col style="width: 9%" />
<col style="width: 3%" />
<col style="width: 3%" />
<col style="width: 9%" />
<col style="width: 9%" />
<col style="width: 9%" />
<col style="width: 9%" />
<col style="width: 9%" />
<col style="width: 9%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="7">ANALYSES REQUESTED</td>
<td colspan="4">ORGANIC</td>
<td colspan="2">INORGANIC</td>
</tr>
<tr class="even">
<td colspan="7">SPECIFIC ANALYSES REQUESTED</td>
<td>VOCs</td>
<td>SVOCs</td>
<td><p>Pesticides/</p>
<p>PCBs</p></td>
<td></td>
<td>Metals</td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">PRESERVATIVES</td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td></td>
<td>None required</td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">ANALYTICAL HOLDING TIME(S)</td>
<td><p>Hold</p>
<p>&lt;2 days</p>
<p>&lt;4 days frozen</p></td>
<td>Hold &lt;14 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;14 days prior to extraction, 40 days after extraction</td>
<td></td>
<td><p>Hold &lt;180 days</p>
<p>(28 days for Hg)</p></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">CONTRACT HOLDING TIMES(S)</td>
<td><p>Hold</p>
<p>&lt;2 days</p>
<p>&lt;4 days frozen</p></td>
<td>Hold &lt;10 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;10 days prior to extraction, 40 days after extraction</td>
<td></td>
<td><p>Hold to 35 days</p>
<p>(26 days for Hg)</p></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">NUMBER OF SAMPLES x NUMBER OF SAMPLE CONTAINERS</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td></td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
</tr>
<tr class="odd">
<td><p>Sample</p>
<p>Number</p></td>
<td><p>Sample</p>
<p>Location</p></td>
<td>Sample Depth</td>
<td><p>Sampling</p>
<p>Date</p></td>
<td>Special Designation</td>
<td colspan="2"><p><u>Conc.</u></p>
<p>Low/Med</p></td>
<td>2 x Encore Samplers</td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td></td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td>TOTAL</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
</tbody>
</table>

**Table 5-2**

**REQUEST FOR ANALYTICAL SERVICES**

**MATRIX = SEDIMENT**

<table>
<colgroup>
<col style="width: 7%" />
<col style="width: 8%" />
<col style="width: 5%" />
<col style="width: 7%" />
<col style="width: 10%" />
<col style="width: 2%" />
<col style="width: 2%" />
<col style="width: 7%" />
<col style="width: 8%" />
<col style="width: 9%" />
<col style="width: 7%" />
<col style="width: 9%" />
<col style="width: 12%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="7">ANALYSES REQUESTED</td>
<td colspan="4">ORGANIC</td>
<td colspan="2">INORGANIC</td>
</tr>
<tr class="even">
<td colspan="7">SPECIFIC ANALYSES REQUESTED</td>
<td>VOCs</td>
<td>SVOCs</td>
<td>Pesticides/PCBs</td>
<td></td>
<td>Metals</td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">PRESERVATIVES</td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td></td>
<td>None required</td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">ANALYTICAL HOLDING TIME(S)</td>
<td><p>Hold</p>
<p>&lt;2 days &lt;4 days frozen</p></td>
<td>Hold &lt;14 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;14 days prior to extraction, 40 days after extraction</td>
<td></td>
<td><p>CLPAS</p>
<p>Metals</p></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">CONTRACT HOLDING TIMES(S)</td>
<td><p>Hold</p>
<p>&lt;2 days</p>
<p>&lt;4 days frozen</p></td>
<td>Hold &lt;10 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;10 days prior to extraction, 40 days after extraction</td>
<td></td>
<td><p>Hold to 35 days</p>
<p>(26 days for Hg)</p></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">NUMBER OF SAMPLES x NUMBER OF SAMPLE CONTAINERS</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td></td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
</tr>
<tr class="odd">
<td><p>Sample</p>
<p>Number</p></td>
<td><p>Sample</p>
<p>Location</p></td>
<td>Sample Depth</td>
<td><p>Sampling</p>
<p>Date</p></td>
<td>Special Designation</td>
<td colspan="2"><p><u>Conc.</u></p>
<p>LOW/MED</p></td>
<td>2 x Encore Samplers</td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td></td>
<td>1 x 8 ounce wide mouth glass jar</td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td>TOTAL</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
</tbody>
</table>

**Table 5-3**

**REQUEST FOR ANALYTICAL SERVICES**

**MATRIX = GROUNDWATER**

<table>
<colgroup>
<col style="width: 4%" />
<col style="width: 6%" />
<col style="width: 4%" />
<col style="width: 6%" />
<col style="width: 5%" />
<col style="width: 4%" />
<col style="width: 4%" />
<col style="width: 10%" />
<col style="width: 10%" />
<col style="width: 11%" />
<col style="width: 11%" />
<col style="width: 9%" />
<col style="width: 10%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="7">ANALYSES REQUESTED</td>
<td colspan="4"><p>CONTRACT LABORATORY PROGRAM ANALYTICAL SERVICES</p>
<p>(CLPAS)</p></td>
<td colspan="2">REGIONAL ANALYTICAL PROGRAM (RAP)</td>
</tr>
<tr class="even">
<td colspan="7">CHEMISTRY TYPE</td>
<td>INORGANICS</td>
<td colspan="3">ORGANICS</td>
<td colspan="2">ORGANICS</td>
</tr>
<tr class="odd">
<td colspan="7">SPECIFIC ANALYSES REQUESTED</td>
<td>Metals</td>
<td>VOCs</td>
<td>SVOCs</td>
<td>Pesticides/PCBs</td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">PRESERVATIVES</td>
<td><p>Add HNO3</p>
<p>to pH&lt;2</p></td>
<td><p>Add 1:1</p>
<p>HCl to pH &lt;2</p>
<p>Chill to 4C</p></td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">ANALYTICAL HOLDING TIME(S)</td>
<td><p>Hold to 6 months</p>
<p>(28 days for Hg)</p></td>
<td><p>Hold</p>
<p>&lt;7 days</p></td>
<td>Hold &lt;7 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;7 days prior to extraction, 40 days after extraction</td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">CONTRACT HOLDING TIMES(S)</td>
<td><p>Hold to 35 days</p>
<p>(26 days for Hg)</p></td>
<td><p>Hold</p>
<p>&lt;5 days</p></td>
<td>Hold &lt;5 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;5 days prior to extraction, 40 days after extraction</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">NUMBER OF SAMPLES x NUMBER OF SAMPLE CONTAINERS</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
</tr>
<tr class="even">
<td><p>Sample</p>
<p>Number</p></td>
<td><p>Sample</p>
<p>Location</p></td>
<td>Sample Depth</td>
<td><p>Sampling</p>
<p>Date</p></td>
<td>Special Designation</td>
<td colspan="2"><p><u>Conc.</u></p>
<p>LOW  MED</p></td>
<td>1 x 1 liter polyethylene bottle</td>
<td><p>3 x 40 mi</p>
<p>glass vials</p></td>
<td><p>2 x 1 liter amber</p>
<p>glass jar</p></td>
<td>2 x 1 liter amber glass jar</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td>TOTAL</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
</tbody>
</table>

**Table 5-4**

**REQUEST FOR ANALYTICAL SERVICES**

**MATRIX = SURFACE WATER**

<table>
<colgroup>
<col style="width: 4%" />
<col style="width: 6%" />
<col style="width: 4%" />
<col style="width: 6%" />
<col style="width: 5%" />
<col style="width: 4%" />
<col style="width: 4%" />
<col style="width: 10%" />
<col style="width: 10%" />
<col style="width: 11%" />
<col style="width: 11%" />
<col style="width: 9%" />
<col style="width: 10%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="7">ANALYSES REQUESTED</td>
<td colspan="4"><p>CONTRACT LABORATORY PROGRAM ANALYTICAL SERVICES</p>
<p>(CLPAS)</p></td>
<td colspan="2">REGIONAL ANALYTICAL PROGRAM (RAP)</td>
</tr>
<tr class="even">
<td colspan="7">CHEMISTRY TYPE</td>
<td>INORGANICS</td>
<td colspan="3">ORGANICS</td>
<td colspan="2">ORGANICS</td>
</tr>
<tr class="odd">
<td colspan="7">SPECIFIC ANALYSES REQUESTED</td>
<td>Metals</td>
<td>VOCs</td>
<td>SVOCs</td>
<td>Pesticides/PCBs</td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">PRESERVATIVES</td>
<td><p>Add HNO3</p>
<p>to pH&lt;2</p></td>
<td><p>Add 1:1</p>
<p>HC1 to pH &lt;2</p>
<p>Chill to 4C</p></td>
<td>Chill to 4C</td>
<td>Chill to 4C</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">ANALYTICAL HOLDING TIME(S)</td>
<td><p>Hold to 6 months</p>
<p>(28 days for Hg)</p></td>
<td><p>Hold</p>
<p>&lt;7 days</p></td>
<td>Hold &lt;7 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;7 days prior to extraction, 40 days after extraction</td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td colspan="7">CONTRACT HOLDING TIMES(S)</td>
<td><p>Hold to 35 days</p>
<p>(26 days for Hg)</p></td>
<td><p>Hold</p>
<p>&lt;5 days</p></td>
<td>Hold &lt;5 days prior to extraction, 40 days after extraction</td>
<td>Hold &lt;5 days prior to extraction, 40 days after extraction</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td colspan="7">NUMBER OF SAMPLES x NUMBER OF SAMPLE CONTAINERS</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
</tr>
<tr class="even">
<td><p>Sample</p>
<p>Number</p></td>
<td><p>Sample</p>
<p>Location</p></td>
<td>Sample Depth</td>
<td><p>Sampling</p>
<p>Date</p></td>
<td>Special Designation</td>
<td colspan="2"><p><u>Conc.</u></p>
<p>LOW  MED</p></td>
<td>1 x 1 liter polyethylene bottle</td>
<td><p>3 x 40 mi</p>
<p>glass vials</p></td>
<td><p>2 x 1 liter amber</p>
<p>glass jar</p></td>
<td>2 x 1 liter amber glass jar</td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td>Total</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
</tr>
</tbody>
</table>

**Table 5-5**

**REQUEST FOR ANALYTICAL SERVICES**

**MATRIX = FISH**

<table>
<colgroup>
<col style="width: 6%" />
<col style="width: 8%" />
<col style="width: 6%" />
<col style="width: 8%" />
<col style="width: 7%" />
<col style="width: 5%" />
<col style="width: 5%" />
<col style="width: 17%" />
<col style="width: 17%" />
<col style="width: 17%" />
</colgroup>
<tbody>
<tr class="odd">
<td colspan="7">ANALYSES REQUESTED</td>
<td colspan="3"><p>REGIONAL ANALYTICAL PROGRAM</p>
<p>(RAP)</p></td>
</tr>
<tr class="even">
<td colspan="7">CHEMISTRY TYPE</td>
<td>INORGANICS</td>
<td colspan="2">ORGANICS</td>
</tr>
<tr class="odd">
<td colspan="7">SPECIFIC ANALYSES REQUESTED</td>
<td><p>RAP</p>
<p>Metals</p></td>
<td><p>RAP</p>
<p>SVOCs</p></td>
<td><p>RAP</p>
<p>Pesticides/PCBs</p></td>
</tr>
<tr class="even">
<td colspan="7">PRESERVATIVES</td>
<td>Freeze to &lt;0C</td>
<td>Freeze to &lt;0C</td>
<td>Freeze to &lt;0C</td>
</tr>
<tr class="odd">
<td colspan="7">ANALYTICAL HOLDING TIME(S)</td>
<td><p>Hold to 6 months</p>
<p>(28 days for Hg)</p></td>
<td>Hold 1 year prior to extraction, 40 days after extraction</td>
<td>Hold 1 year prior to extraction, 40 days after extraction</td>
</tr>
<tr class="even">
<td colspan="7">CONTRACT HOLDING TIMES(S)</td>
<td>Not applicable</td>
<td>Not applicable</td>
<td>Not applicable</td>
</tr>
<tr class="odd">
<td colspan="7">NUMBER OF SAMPLES x NUMBER OF SAMPLE CONTAINERS</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
<td>No. of Containers per Analysis</td>
</tr>
<tr class="even">
<td><p>Sample</p>
<p>Number</p></td>
<td><p>Sample</p>
<p>Location</p></td>
<td>Sample Depth</td>
<td><p>Sampling</p>
<p>Date</p></td>
<td>Special Designation</td>
<td colspan="2"><p><u>Conc.</u></p>
<p>LOW  MED</p></td>
<td>1 x 8 ounce foil wrapped filet</td>
<td>1 x 8 ounce foil wrapped filet</td>
<td>1 x 8 ounce foil wrapped filet</td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>X</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="even">
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
<tr class="odd">
<td>Totals</td>
<td></td>
<td></td>
<td></td>
<td></td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
<td>0</td>
</tr>
</tbody>
</table>

If fish are to be composited, provide 3 x 8 oz. filets, 8 oz. from each
fish. If whole fish are sent, adjust table accordingly.