**31^st^ Annual**

**Eastern Fish Health Workshop**

27 -- 31 March 2006

Holiday Inn -- Patriot's Point

Mt. Pleasant, South Carolina

National Fish Health Research Laboratory

USGS - Leetown Science Center

11649 Leetown Road

Kearneysville, West Virginia 25430

![](media/image1.jpeg){width="1.6041666666666667in"
height="0.6472222222222223in"}

**[Program Committee]{.underline}**

**Rocco C. Cipriano,** U.S. Geological Survey, Leetown Science Center,
National

***chairman*** Fish Health Research Laboratory, 11649 Leetown

> Road, Kearneysville, WV 25430

**Robert Bakal** U.S. Fish and Wildlife Service, 5308 Spring Street,

> Warm Springs, GA 30263

**Thomas A. Bell** U.S. Fish and Wildlife Service, Aquatic Animal Drug

> Approval Partnership, 4050 Bridger Canyon Road, Bozeman, Montana 59715

**Erin J. Burge** Hollings Marine Laboratory, 331 Fort Johnson Road,

> Charleston, SC 29412

**Al Camus** Aquatic Diagnostic Laboratory, DREC, P. O. Box 197,

Stoneville, MS 38776

**Kathleen Hughes** U.S. Department of Agriculture, APHIS-VS, UF,
Tropical **Hartman** Aquaculture Laboratory, 1408 24th Street, S.E.,
Ruskin,

> FL  33570-5434

**John Lumsden** Dept. of Pathology and Microbiology, Ontario Veterinary

College, Guelph, Ontario, Canada, N1G 2W1

**Susan Marcquenski** Wisconsin Department of Natural Resources, 101
South

Webster Street, Box 7921, Madison, WI 53707

**Garriet W. Smith** Biology Department, University of South Carolina,
Aiken,

> SC 29801

##### Weber, Scott New England Aquarium, Central Wharf , Boston, MA 02110-3399

***[Editorial Note]{.underline}***

Thirty-one years ago, a small group of federal researchers gathered
together on the Eastern Shore of Maryland to share their research,
discuss issues, plan new studies, and enjoy the fellowship of each
other. That first meeting was originally inspired by Dr. Stanislaus
Snieszko of the Eastern Fish Disease Laboratory (predecessor to the
National Fish Health Research Laboratory) of the Department of the
Interior and Drs. Carl Sinderman and Aaron Rosenfeld of the National
Marine Fisheries Service Laboratory of the Department of Commerce at
Oxford, Maryland. Both laboratories were somewhat close geographically
and both researched problems associated with fish disease, but their
emphasis differed between the freshwater and marine environment. The
early workshops were a focal point that fostered communication between
the two facilities and provided staff members with opportunities to plan
and conduct collaborative studies. Within a few short years, fish
disease research became well established in the graduate programs of
several major universities in the eastern United States and
participation in the workshop began to evolve beyond its original
intent. Although the title of the workshop appears to be exclusive for
fish, the workshop actually encourages much broader participation in all
aspects of aquatic animal health from shellfish to mammals. Furthermore,
the workshop remains true to its roots and encourages contributions from
researchers, resource managers, biologists, and university personnel
working within both marine and freshwater environments. Even though the
Workshop has occasionally been held in conjunction with other
organizations such as the Fish Health Section of the American Fisheries
Society and the International Association of Aquatic Animal Medicine, it
is not affiliated with any parent organization and the meeting is an
entity unto itself. Today, the National Fish Health Research Laboratory
is extremely proud to continue the tradition of the Eastern Fish Health
Workshop and serve as its annual host. The staff of the National Fish
Health Research Laboratory welcomes you to the 31^st^ Annual Eastern
Fish Health Workshop at the Holiday Inn and Conference Center of
historic Mount Pleasant, South Carolina.

Robert N. Rosen wrote in [A short history of Charleston]{.underline},
that \"The beauty of Charleston mesmerizes people and makes it difficult
for them to visualize the morality play that is Charleston\'s history.
There is good and evil in that history, for the city is not innocent. Is
Sullivan's Island the Ellis Island of black America? Or is it the scene
of Edgar Allan Poe's \"The Gold Bug\"? Or is it just a nice oceanfront
suburb? Is the Old Exchange Building a fancy architectural jewel
designed to house 18th-century assemblies? Or is it the ghoulish prison
of the Revolution, the place where the martyr Isaac Hayne spent his last
night? Or is it the place where George Washington greeted his fellow
citizens? And there is no question that slaves were sold for generations
next to the very balcony from which the Declaration of Independence was
read. It is the tragic and ironic aspects of Charleston s history that
give it such power. It resembles the classic Greek tragedy- its
aristocratic and noble leaders were plagued by fatal flaws, by hubris,
by pride, by something. They foresaw the decline of the aristocracy. As
early as 1833, Hugh Legare wrote: \"We are (I am quite sure) the last of
the race of South Carolina.\"

Perhaps the drama was played out in the Civil War, which, as Sidney
Andrews said, left \"a city of ruins. \"For it is, after all the fall of
the noble hero that is the essence of Greek tragedy. When Henry James
visited Charleston in 1905, some forty years after the Civil War had
ended, he also was captivated by the antique quality of life, by \"the
Battery of the long, curved seafront, of the waterside public garden,
furnished with sad old historic guns; by Fort Sumter and the start of
the Civil War. \"The Forts, faintly blue on the twinkling sea," he
wrote, \"looked like vague marine flowers; innocence, pleasantness ruled
the prospect.\" James, a northerner, was reacting to the start of the
Civil War - the firing on the flag at Sumter. But Fort Sumter is so far
from the Battery. \"The Flag would have been, from the Battery,\" he
concluded, \"such a mere speck in space. There is more. Charleston\'s
history has a power even beyond the supposed climax, defeat in the Civil
War\...\"

Some have written that there is just no way to completely explain all of
the beauty and contradictions that abound in this historic jewel of the
South, but in the days ahead, we hope that you will have the time to
venture beyond the hotel and sample the enduring lore, legends, and
history of is beautiful, southern seaport.

> **Rocco C. Cipriano**

**[Monday]{.underline} [March 27, 2006]{.underline}**

5:00 -7:00 **Registration in the Seabrook Room**

Social Reception

Cash Bar

**[Tuesday]{.underline} [March 28, 2006]{.underline}**

7:00 **Breakfast: The Charleston Buffet**

> **Jericho Room**
>
> *Chilled Orange Juice, Sliced Fresh Fruit, Buttermilk Pancakes,
> Country Scrambled Eggs, Hickory Smoked Bacon, Hash Brown Potatoes,
> Danishes*
>
> *And Muffins, Cereals, Coffee And Assorted Teas*

**[General Session 1:]{.underline} Clifford E. Starliper, moderator**

8:00 Darwish Laboratory Efficacy Of Florfenicol Against *Streptococcus
iniae* 1

> Infection In Sunshine Bass

8:15 Zhang Comparison Of Lipopolysaccharide And Protein Profiles Between
2

> *Flavobacterium columnare* Strains From Different Genomovars

8:30 Starliper Evaluation Of Medium Components For Growth Of
*Flavobacterium* 3 *psychrophilum*

8:45 Hesami Antimicrobial Resistance Patterns Of Ontario
*Flavobacterium* 4

> *psychrophilum* Strains

9:00 Arias Application Of Polyphasic Taxonomy To The Study Of Bacterial
5

> Fish Pathogens

9:15 Brown Using DNA Microarrays To Compare Clinical Isolates Of The 6

> Fish Pathogen *Aeromonas salmonicida*.

9:30 Dacanay From Sequence To Sickness: Pathogenesis In *Aeromonas* 7

> *salmonicida* subsp. *salmonicida*

9:45 Loch Bacterial Infections In Representative Lake White Fish 8

> *(Coregonus clupeaformis)* Stocks In Michigan

10:00 **Coffee, tea**

**[General Session 2:]{.underline} Harry Dickerson, moderator**

10:30 Young, K. Quantification Of A 16kda Defense Lectin (Ladderlectin)
In 9

> Rainbow Trout (*Oncorhynchus mykiss*) Plasma

10:45 Russell Characterization Of Ladder Lectin And An Intelectin In 10

> The Plasma Of Rainbow Trout (*Oncorhynchus mykiss*)

11:00 Zhao B Lymphocytes And Antibody-Secreting Cells In Channel 11

> Catfish Skin

11:15 Ye The Impacts Of Redox Structure On Trout Antibody Affinity 12

11:30 Smith S. Characterization And Utilization Of Affinity-Purified
Atlantic 13

> Sturgeon (*Acipenser oxyrinchus*) Immunoglobulin

# 11:45 Baker The Health Condition Of Impinged And Open Water Fish 14

# From The Mobile River

12:00 **Lunch in the Jericho Room**

> *Caesar Salad, Southern Fried Chicken Served With Creamy Mashed*
>
> *Potatoes, Green Beans, Rolls And Butter, Coffee And Tea*

**[Welcome:]{.underline}**

1:00 Panek **Welcome**

**[Special Session 1:]{.underline} Health Management And Clinical
Conundrums In Public Aquaria**

> **Scott Weber, moderator**

1:15 Thorvalson The South Carolina Aquarium Sea Turtle Rescue 15

1:30 Young, D. Characterization Of A Systemic Iridovirus From The 16

> Banggai Cardinalfish (*Pterapogon kauderni*)

1:45 Postell *Coryphaena hippurus* - The Challenges Of Collecting And 17

> Exhibiting This Pelagic Species

2:00 Keller Amyloodinium In Elasmobranchs 18

2:15 Innis Laparoscopic Liver Biopsy In Fish: Equipment, Technique, 19

> And Preliminary Assessment Of Biopsy Quality

2:30 Gore Challenges of Managing Horseshoe Crabs (*Limulus polyphemus*)
20

> In An Interactive Exhibit

2:45 Weber Medical Management Of Cold-Stunned Sea Turtles Stranded 21

On Cape Cod

3:00 **Coffee, Tea, Soda**

**[Special Session 2:]{.underline} Challenges For Vaccination In Aquatic
Animal Health**

> **John Lumsden, moderator**

3:30 Bromage Vaccine Design 22

3:45 Panagala Beneficial Use Of Attenuated Vaccines In Aquaculture 23

4:00 Simard DNA Vaccines And Environmental Safety 24

4:15 Bootland IPNV Cohabitation Challenge Of Atlantic Salmon Post-Smolts
25

4:30 Dickerson *Ichthyophthirius* *multifiliis*: Challenges In Vaccine
26

Development

4:45 Clark Structural Analysis And Epitope Mapping Of *Ichthyophthirius*
27

> I-Antigens

5:00 **Adjourn**

6:30 Car pool From the hotel lobby

7:30 **Evening Tour: Charleston in the Civil War**

> Charleston\'s Civil War experience is based on *The Bombardment of
> Charleston*, authored by the Civil War Walk\'s founder and lead
> guide - Chris Phelps. Charleston enacted the opening scene of the
> conflict by hosting the secession convention in December 1860, which
> led South Carolina out of the Union, the first of eleven Southern
> states to withdraw. Four months later, in April of 1861, war was
> inaugurated in Charleston harbor when Southern forces fired on the
> U.S. garrison in Fort Sumter. Charleston paid an ultimate price for
> these actions when the might of the Union Army and Navy descended on
> her in 1863. Come with us to hear the story that followed this fateful
> time in Charleston's history. The tour is conducted by \"America On
> Foot,\" lasts approximately two hours, and covers a distance of 1.5
> miles. We shall meet our guides in Washington Park at the corner of
> Broad and Meetings Streets. Due to certain city ordinances placing
> restrictions on the number of individuals per tour guide, reservations
> must have been made in advance, therefore, tickets cannot be sold at
> the door.

**[Wednesday]{.underline} [March 29, 2006]{.underline}**

7:00 **Breakfast: The Charleston Buffet**

**Jericho Room**

> *Chilled Orange Juice, Sliced Fresh Fruit, Buttermilk Pancakes,
> Country Scrambled Eggs, Grilled Link Sausage, Creamy Grits, Bagels and
> Cream Cheese, Cereals, Coffee And Assorted Teas*

**[Special Session 3:]{.underline} Advances In Crustacean Health And
Disease**

**Erin Burge, moderator**

8:00 Shields Ecological Determinants In Outbreaks Of Bitter Crab Disease
28

> (*Hematodinium* sp.) In Snow Crabs, *Chionoecetes opilio,* From
>
> Conception Bay, Newfoundland, Canada

8:15 Lee Molecular Diagnostics For *Hematodinium* And Other Protozoa 29

> In Water And Crustacean Hosts

8:30 Burge Lysozyme Gene Expression As A Molecular Marker Of Hemocyte 30

> Location In Pacific White Shrimp, *Litopenaeus vannamei,* After
>
> Injection With *Vibrio*

8:45 Burnett, L. Changes In Metabolism And Performance In Crustaceans 31

> Fighting Bacterial Infections

9:00 Shepard White Spot Syndrome Virus (WSSV)In Wild *Litopenaeus
setiferus* 32

> And *Callinectes sapidus* From The U.S. South Atlantic Coast

9:15 Li Diagnosis Of *Panulirus argus* Virus 1 (Pav1) In The Caribbean
33

> Spiny Lobster Using Fluorescence *In Situ* Hybridization

9:30 Messick An Overview Of Disease Surveys In Crustaceans From The 34

> Chesapeake Bay And United States Mid-Atlantic Coast

9:45 Oweson Manganese Induced Immune Suppression in *Nephrops
norvegicus* 35

10:00 **Coffee, tea**

**[Special Session 4:]{.underline} What\'s Wrong With My Fish: Clinical
Approaches In Pet Fish Medicine**

**Robert Bakal, moderator**

10:30 Tepper Clinical Aspects Of Water Biology And The Effects On Water
36

> Quality In Koi Ponds

10:45 Yosha Selected Cases In Fish Medicine In Private Practice 37

11:00 Roberts Frozen Fish: Private Fish Medicine In Buffalo, NY 38

11:15 Wolf Dermal Mesenchymal Neoplasms In Goldfish, Koi And Carp 39

11:30 Lewbart Laser Surgery To Remove Skin Tumors In Goldfish
(*Carassius* 40

*auratus*)

11:45 Aiken Safe and Effective Control of Ozone in Aquarium Life 41

> Support Systems

12:00 **Lunch In the hotel restaurant**

> *Pan Seared Shrimp And Garden Vegetables With Creamy Red Bell Pepper
> Pesto Sauce Served Over Fettuccine with Mixed Greens And Parmesan*
>
> *Dressing, Rolls And Butter, Coffee And Tea.*

**[Special Session 5:]{.underline} Imminent threats affecting our coral
reefs**

> **Garriet Smith, moderator**

1:00 Johnston The Microbial Diversity Associated With The Gorgonian 42

> Coral, *Pseudopterogorgia americana*

1:15 Polson Assessment Of Microbial Communities Associated With 43

> An Acroporid Coral Disease Outbreak

1:30 Lipp Investigations Of *Serratia marcescens* As A White Pox 44

> Pathogen In The Florida Keys

1:45 Schill Coral Disease And Genetics Survey In St. John, USVI Using 45

> Non-Destructive Methods

2:00 Gil-Agudelo Zooxanthellae Shifts And Yellow Band Disease 46

2:15 Smith G. White Band Disease In The Caribbean 47

2:30 Sekar Molecular Diversity Of Microbes Associated With Black 48

> Band Diseased Coral *Siderastrea siderea*

2:45 Pinzón Defining The Reservoir Of The Pathogen *Aurantimonas
coralicida* 49

> By Fluorescence *In Situ* Hybridization

3:00 **Coffee, Tea, Soda**

3:30 Cook Differential Diagnosis: A Need For The Use Of
Multidisciplinary 50

> Tools To Investigate The Coral Disease White Plague Type II

[General Session 3:]{.underline} Jill Rolland, moderator

3:45 Mitchell From Leidy To Snieszko: Pioneers Of Fish Health In The
United 51

> States (1850 - 1950)

4:00 Egrie The Current Status Of The United States National Aquatic
Animal 52

> Health Plan

4:15 Kebus Fish Health Medicine Certificate Program 53

4:30 Pasnik Natural Content And Processing of Alternative Protein
Sources: 54

> Histologic Effects In Fingerling Channel Catfish (*Ictalurus*
>
> *punctatus*)

4:45 Shoemaker Growth Response and Acquired Resistance of
*Streptococcus* 55

> *iniae*-recovered Nile Tilapia, *Oreochromis* *niloticus*

**Adjourn**

5:30 Car Pool from hotel lobby

6:00 **Evening Tour South Carolina Aquarium**

> Jason Crichton, host
>
> Charleston, SC
>
> (Everyone welcome, no reservation required)

**[Thursday]{.underline} [March 30, 2006]{.underline}**

7:00 **Breakfast: The Charleston Buffet**

**The Jericho Room**

> *Chilled Orange Juice, Sliced Fresh Fruit, Buttermilk Pancakes,
> Country Scrambled Eggs, Hickory Smoked Bacon, Hash Brown Potatoes,
> Danishes*
>
> *And Muffins, Cereals, Coffee And Assorted Teas*

**[Case Reports:]{.underline} The Puzzling, The Weird, The Unknown:
Sharing Your Diagnosis**

**Sue Marcquenski and Al Camus**, **moderators**

8:00 Camus Hurricane Katrina And The Mississippi Catfish Industry, 56

> A Little Good News For A Change

8:10 Keller Bacterial Endocarditis In A Giant Pacific Octopus, 57

*Enteroctopus dofleini*

8:20 Govett Enrofloxacin Resistant Meningoencephalitis In A Tiger Shark
58

> (*Galeacerdo cuvier*)

8:30 Bowser Koi Herpesvirus In New York State: The 2005 Common Carp 59

> Mortality Event

8:40 Yazdanpanah Pancreatic Amyloidosis In Captive Tricolour Sharks 60

8:50 Lowry Progressive Erosive Stomatitis In Green Moray Eels: A Case
Study 61

9:00 Mitchell The Big Belly Blues 62

9:10 Khoo Pathology Associated With *Coleps* Spp. In Tilapia 63

9:20 Durburow Three Disease Cases: Acid-Sulfate Soil, Largemouth Bass 64

> Wintertime Sudden Death, And Channel Catfish "Pimples**"**

9:30 Bowser Unusual Spinal Deformity In Newly Hatched Yellow Perch 65

9:40 Pasnik Atypical Clinical Signs of *Streptococcus agalactiae* in
Nile 66

> Tilapia (*Oreochromis niloticus*)

9:50 Peters A Tale of Two Tumors: The Shrimp's And The Clam's 67

10:00 **Coffee, tea**

**[General Session 4:]{.underline} Drew Mitchell, moderator**

10:30 Small Molecular Detection And Identification Of *Hematodinium*
spp. 68

10:45 Wheeler Aspects Of The Pathology Of *Hematodinium* Infections In
Snow 69

> Crabs (*Chionoecetes opilio*) From Newfoundland, Canada

11:00 Burnett, K. Effects Of Hypercapnic Hypoxia On Clearance Of
*Vibrio* 70

> *campbellii* In The Eastern Oysters, *Crassostrea virginica*

11:15 Xu Putative Identification Of Expressed Genes Associated With 71

> The Attachment Of The Zebra Mussel (*Dreissena polymorpha*)
>
> Using Suppression Subtractive Hybridization cDNA Library

11:30 Fast Recent Advances In Oue Understanding The Host-Parasite 72

> Relationship Of *Lepeophtheirus salmonis* And Atlantic Salmon
>
> (*Salmo Salar*)

11:45 Dove Unusual Trematode "Egg Nests" In The Stomach Tissues Of 73

> Goggle-Eyed Scad, *Selar crumenophthalmus* (Bloch, 1793)

12:00 **Lunch Jericho Room**

> *Sliced Char-Grilled Breast Of Chicken Over Fresh Romaine Lettuce
> With*
>
> *Croutons, Parmesan Cheese And Caesar Dressing, Key Lime Pie, Coffee*
>
> *And Tea*

**[General Session 5:]{.underline} Janet Warg, moderator\
**

1:00 Panek Disease And Adaptive Fishery Management: The Role Of The 74

> Fish Health Professional

**[Special Session 6:]{.underline} New Aquaculture Drugs: Oxymoron,
Myth, Or Reality**

> **Tom Bell, moderator**

1:30 Bell Drugs For Domestic Aquaculture: Background And 75

Session Strategy

1:45 Schnick Drug Approval Status: Then And Now 76

2:00 Prater The INAD/NADA Process: That\'s Not A Train, But A Light 77

> At The End Of The Tunnel

2:15 Bowker Efficacy And Target Animal Safety Studies: Who Said This 78

> Was A Cake Walk?

2:30 Meinertz Trials And Tribulations Developing Environmental Safety

> And Human Food Safety Data For Aquaculture Drug Approvals 79

2:45 Schnick Drug Approval Status: Why We Are Where We Are And Not 80

> Where You Thought We Should Be

3:00 **Coffee, tea, soda**

**[General Session 6:]{.underline} Laura Brown, moderator**

3:30 Lewis Monitoring Hawaiian Invertebrates (Crustacea) For White Spot
81

> Syndrome Virus (WSSV)

3:45 Densmore Comparative Susceptibility Of Early Life Stages Of The
Bullfrog 82

> (*Rana catesbeiana*) To The Tadpole Edema Virus

4:00 Bowser Largemouth Bass Virus In New York State 83

4:15 Faisal Isolation Of Viral Hemorrhagic Septicemia Virus (VHSV) From
84

> Muskellunge (*Esox masquinongy*) From Lake St. Clair, Michigan

4:30 Lumsden Isolation Of Viral Hemorrhagic Septicemia Virus Type IV 85

> From A Mortality Event In Lake Ontario Drum
>
> (*Aplodinotus grunniens*)

4:45 Cipriano Kinetics Of The Natural Microbial Populations Of Atlantic
86

> Salmon In Relation To Infection And The Recuperative Process

5:00 **Adjourn**

7:00 **Reception**

7:30 **Banquet**

> *Seafood Medley Over Fettuccini With Fresh Vegetables, Sliced Sirloin
> Of Beef In Brandy Sauce, Chicken Stuffed With Cornbread In A Supreme
> Sauce, Tossed Seasonal Salad, Roasted Red Potatoes, Seasonal Vegetable
> Medley, Chef's Choice Of Assorted Desserts, Coffee And Iced Tea*
>
> **Best Student Paper Award**

8:30 **Social and dance**

11:30 **Adjourn**

**[Friday]{.underline} [March 31, 2006]{.underline}**

7:00 Breakfast (for participants of the Continuing Education Course)

**The Charleston Buffet**

> *Chilled Orange Juice, Sliced Fresh Fruit, Buttermilk Pancakes,
> Country Scrambled Eggs, Grilled Link Sausage, Hash Brown Potatoes,
> Bagels and Cream Cheese, Cereals, Coffee And Assorted Teas*

8:00 Hartman Welcome And Introductions

8:15 Christy Emergency Coordination -- The Florida SART Program

9:00 Rolland Response To Foreign Aquatic Animal Diseases

9:45 Hartman On Farm Preparedness For Natural Disasters

10:15 **Coffee, tea**

10:30 Gustafson Aquaculture Biosecurity, Disinfection, Carcass Disposal

Ellis

11:15 Roberts Emergency Needs Of The Private Pet Fish/Pond Owner

12:00 **Lunch**

> *Marinated sliced sirloin topped with a brandy mushroom sauce served
> with roasted new potatoes and mixed greens with creamy parmesan
> dressing, French style green beans, rolls and butter, coffee and tea*

1:00 Sima Emergency Preparedness For Public Aquariums

1:45 Sillitti Emergency Preparedness For Aquatic Research Laboratories

2:30 **Informal** **Break**

2:40 Vannieu- ICS structure and overview

wenhoven

3:00 Vannieu- Emergency Scenario exercise

wenhoven

4:30 Hartman Discussion, Summary, Evaluation

5:00 **Adjourn**