REPORTS OF THE COORDINATORS

Overall coordinator's report

**Udda Lundqvist**

**Nordic Gene Bank**

**P.O. Box 41. SE-230 53 Alnarp**

**e-mail: udda@nordgen.org**

Since the latest overall coordinator's report in Barley Genetics
Newsletter Volume 36, some changes of the coordinators have taken place.
I do hope that most of you are willing to continue with this work and
provide us with new important information and literature search in the
future. A replacement was found for Chromosome 4H, namely Arnis Druka,
Genetics Programme at the Scottish Crop Research Institute, Invergowrie,
Dundee, United Kingdom. Please observe some address changes have taken
place since the last volume of BGN. Jerry Franckowiak, the Coordinator
for chromosome 2H, the semi-dwarf collection and all his immense efforts
creating isogenic lines in the Bowman genetic background of many
different barley genetic stocks has moved from North Dakota State
University to Warwick, Queensland, Australia. The Curator, An Hang, for
the Barley Genetics Stock Center at the USDA-ARS station at Aberdeen,
Idaho, USA, has retired during the year 2007. Dr. Harold Bockelman from
the same station is nominated as successor. An Hang has been involved
and engaged in Barley Genetics since many decades, first together with
Tak Tsuchiya at Fort Collins, Colorado and since 1990 at Aberdeen,
Idaho. He took care of the move of all genetic barley stocks from Fort
Collins to Aberdeen, has been evaluating and increasing most of them. He
has been a considerable collaborator and colleague to the barley
community, handled with big carefulness all the different barley types
and transferred a large knowledge to all of us. I take this opportunity
to thank him for all his kindness, helpfulness, enthusiasm and
inspiration during all these years. All the best wishes to him in the
future and his retirement. But I want to thank those who have resigned
for their good corporation and the reliability of sending informative
reports during all the years.

In this connection I also want to call upon the barley community to pay
attention on the AceDB database for 'Barley Genes and Barley Genetic
Stocks'. It contains much information connected with images and is
useful for barley research groups inducing barley mutants and looking
for new characters. It gets updated continuously and some more images
are added to the original version. The searchable address is:
[www.untamo.net/bgs](http://www.untamo.net/bgs)

In some months the 10th International Barley Genetics Symposium will be
organized in Alexandria, Egypt. I hope that many of you will be to
participate in the meetings. It is of big importance to discuss the
future of different items, especially the coordination system and the
future of Barley Genetics Newsletter. I would like to encourage the
coordinators and their colleagues already to-day to provide me with
suggestions, ideas, items or topics to be brought up during the
meetings.

**List of Barley Coordinators**

**Chromoosome 1H (5):** Gunter Backes, The University of Copenhagen,
Faculty of Life Science, Department of Agricultural Sciences,
Thorvaldsensvej 40, DK-1871 Fredriksberg C, Denmark. FAX: +45 3528 3468;
e-mail: \<guba[\@life.ku.dk\>]{.underline}

**Chromosome 2H (2):** Jerry. D. Franckowiak, Hermitage Research
Station, Queensland Department of Primary Industries and Fisheries,
Warwick, Queensland 4370, Australia, FAX: +61 7 4660 3600; e-mail:
\<[Jerome.franckowiak@dpi.qld.gpv.au]{.underline}\>

**Chromosome 3H (3):** Luke Ramsey, Genetics Programme, Scottish Crop
Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX:
+44 1382 562426. E-mail: \<Luke.Ramsey@scri.sari.ac.uk\>

**Chromosome 4H (4):** Arnis Druka, Genetics Programme, Scottish Crop
Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX:
+44 1382 562426. e-mail: \<[adruka@scri.sari.ac.uk]{.underline}\>

**Chromosome 5H (7):** George Fedak, Eastern Cereal and Oilseed Research
Centre, Agriculture and Agri-Food Canada, ECORC, Ottawa, ON, Canada K1A
0C6, FAX: +1 613 759 6559; e-mail: \<[fedakga@agr.gc.ca]{.underline}\>

**Chromosome 6H (6):** Duane Falk, Department of Crop Science,
University of Guelph, Guelph, ON, Canada, N1G 2W1. FAX: +1 519 763 8933;
e-mail: \<[dfalk@uoguelph.ca]{.underline}\>

**Chromosome 7H (1):** Lynn Dahleen, USDA-ARS, State University Station,
P.O. Box 5677, Fargo, ND 58105, USA. FAX: + 1 701 239 1369; e-mail:
\<[DAHLEENL@fargo.ars.usda.gov]{.underline}\>

**Integration of molecular and morphological marker maps:** Andy
Kleinhofs, Department of Crop and Soil Sciences, Washington State
University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail:
\<[andyk@wsu.edu]{.underline}\>

**Barley Genetics Stock Center:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<<anhang@uidaho.edu>[\>]{.underline}

and

Harold Bockelmann, National Small Grains Collection, U.S. Department of
Agriculture -- Agricultural Research Service, 1691 S. 2700 W., Aberdeen,
ID 83210, USA. FAX: +1 208 397 4165. e-mail:
\<[nsgchb@ars-grin.gov\>]{.underline}

**Trisomic and aneuploid stocks:** An Hang, USDA-ARS, National Small
Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210,
USA. FAX: +1 208 397 4165; e-mail: \<[anhang@uidaho.edu]{.underline}\>

**Translocations and balanced tertiary trisomics:** Andreas Houben,
Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3,
DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail:
\<houben@ipk-gatersleben.de\>

**List of Barley Coordinators (continued)**

**Desynaptic genes:** Andreas Houben, Institute of Plant Genetics and
Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany.
FAX: +49 39482 5137; e-mail: \<houben@ipk-gatersleben.de\>

**Autotetraploids:** Wolfgang Friedt, Institute of Crop Science and
Plant Breeding, Justus-Liebig-University, Heinrich-Buff-Ring 26-32,
DE-35392 Giessen, Germany. FAX: +49 641 9937429; e-mail:
\<[wolfgang.friedt@agrar.uni-giessen.de]{.underline}\>

**Disease and pest resistance genes:** Brian Steffenson, Department of
Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper
Buford Circle, St. Paul, MN 55108-6030, USA. FAX: +1 612 625 9728;
e-mail: \<[bsteffen@umn.edu]{.underline}\>

**Eceriferum genes:** Udda Lundqvist, Nordic Gene Bank, P.O. Box 41,
SE-230 53 Alnarp, Sweden. FAX:.+46 40 536650; e-mail: \<
[udda@nordgen.org]{.underline}\>

**Chloroplast genes:** Mats Hansson, Department of Biochemistry,
University of Lund, Box 124, SE-221 00 Lund, Sweden. FAX: +46 46 222
4534 e-mail: \<mats.hansson@biochemistry.lu.se\>

**Genetic male sterile genes:** Mario C. Therrien, Agriculture and
Agri-Food Canada, P.O. Box 1000A, R.R. #3, Brandon, MB, Canada R7A 5Y3,
FAX: +1 204 728 3858; e-mail: \<MT[herrien@agr.gc.ca]{.underline}\>

**Ear morphology genes:** Udda Lundqvist, Nordic Gene Bank, P.O. Box 41,
SE-230 53 Alnarp, Sweden. FAX: +46 40 536650; e-mail: \<
[udda@nordgen.org](mailto: udda@nordgen.org)[\>]{.underline}

or

Antonio Michele Stanca: Istituto Sperimentale per la Cerealicoltura,
Sezione di Fiorenzuola d'Arda, Via Protaso 302, IT-29017 Fiorenzuola
d'Arda (PC), Italy. FAX +39 0523 983750, e-mail:
\<[michele@stanca.it]{.underline}\>

**Semi-dwarf genes:** Jerry D. Franckowiak, Hermitage Research Station,
Queensland Department of Primary Industries and Fisheries, Warwick,
Queensland 4370, Australia, FAX: +61 7 4660 3600; e-mail: \<
[Jerome.franckowiak@dpi.qld.gpv.au]{.underline} \>

**Early maturity genes:** Udda Lundqvist, Nordic Gene Bank, P.O. Box 41
SE-230 53 Alnarp, Sweden. FAX: +46 40 536650; e-mail:
\<[udda@nordgen.org]{.underline}\>

**Biochemical mutants - Including lysine, hordein and nitrate
reductase:** Andy Kleinhofs, Department of Crop and Soil Sciences,
Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509
335 8674; e-mail: \<[andyk@wsu.edu]{.underline}\>

**Barley-wheat genetic stocks:** A.K.M.R. Islam, Department of Plant
Science, Waite Agricultural Research Institute, The University of
Adelaide, Glen Osmond, S.A. 5064, Australia. FAX: +61 8 8303 7109;
e-mail: \<[rislam@waite.adelaide.edu.au]{.underline}\>

**Coordinator's Report: Barley Chromosome 1H (5)**

**Gunter Backes**

**The University of Copenhagen\
Faculty of Life Sciences**

**Department of Agricultural Sciences**

**Thorvaldsensvej 40\
DK-1871 Frederiksberg C, Denmark**

**[guba@life.ku.dk]{.underline}**

In Arabidopsis, *HLM1* encodes the cyclic nucleotide-gated ion channel
4. A mutant plant for this gene shows necrotic lesions and thereby
similarities to the hypersensitive response (HR) to pathogens. Rostoks
*et al*. (2006) isolated the homolog of this gene from the previously
characterized barley mutant *nec1* and localized the gene in the
'Steptoe' x 'Morex' "minimapper population" to chromosome 1H, Bin9.

In an attempt to localize transcription factors (TFs ) belonging to the
gene family of C-repeat binding factors (CBF), their regulators and
MYB-TFs, altogether known to regulate plant response to cold and drought
stress, Tondelli *et al*. (2006) localized several homologs to the
respective *Arabidopsis* genes in a joined map of the three populations
'Nure' × 'Tremois', 'Proctor' × 'Nudinka' and 'Steptoe' × 'Morex'.
Further, they compared the loci of these putative TFs with the position
of published QTLs. They localized 9 homologs and assigned two further
homologs by wheat-barley addition lines to the respective chromosomes.
On chromosome 1H they localized *HvMYB4* to Bin6, a homolog to *AtMyb2*
from *Arabidopsis* and *OsMYB4* from rice, both known to be part of the
regulation processes during abiotic stresses.

In a similar effort, Skinner *et al*. (2006) localized the barley
homologs of 14 *Arabidopsis* CBF-TFs and 2 further TFs in the barley
populations 'Steptoe' × 'Morex' and 88Ab536 × 'Strider'.The authors also
tested the 'Steptoe' × 'Morex' population in climatic chambers for cold
tolerance and localized QTLs based on these data. On chromosome 1H, they
localized *HvZFP16-1*, a homolog to *AtZAT12*, to Bin4. A further
homolog of the same Arabidopsis gene, *HvZFPR16-2* was assigned to 1H by
wheat-barley addition lines. A QTL for cold tolerance was localized on
1H, Bin11, by a LOD score of 5.7. No co-localization between the QTLs
and the TFs localized in this study was found. Nevertheless, comparison
with literature indicated QTLs at the position of two candidate gene
loci on 5H.

The same group (Szücs *et al*. 2006) published results describing the
localization of photorecep­tor genes and vernalization-related genes
together with QTLs for photoperiod response. Mapping of both, the
candidate genes and QTLs, was carried out in DH populations of the
crosses 'Dicktoe' × 'Morex' and 'Dicktoe' × 'Kompolti korai'. While none
of the candidate genes was localized on chromosome 1H, two QTL were
detected with the 'Dicktoo' × 'Morex' population: a major QTL in Bin11
and a further QTL in Bin12.

In order to localize qualitative and quantitative resistance against
rice blast in barley, Inukai *et al*. (2006) analyzed a segregating DH
population from the cross 'Baroness' × BCD47 with two different rice
blast isolates in a greenhouse experiment. For one of the isolates, a
qualitative segregation was found and consequently a new resistance
gene, *RMo1*, was localized on chromosome 1H, Bin2 at or near the
position of the *Mla*-locus. For the other isolate, a quantitative
segregation was found and a major QTL was detected at the position of
*RMo1*, while further 3 QTLs were localized on the chromosomes 3H, 4H
and 7H.

Jafary *et al*. (2006) investigated the inheritance and specificity of
plant factors that determine the degree of basal defence by host- and
nonhost pathogens. For this purpose, they analyzed 152 RILs from the
cross 'Vada' x 'SusPtrit' with 2 rust isolates from barley rusts and 8
isolates from rusts with no barley-specificity, isolated from cultivated
and wild *Poaceae*. 'SusPtrit' is an experimental barley accession
selected for susceptibility to the wheat leaf rust fungus *Puccinia
triticina*. On chromosome 1H, an R-gene against the fungus *Puccinia
hordei-secalini* was localized. *P. hordei-secalini* has no
host-specificity for *H. vulgare*. Furthermore, three different QTLs
were detected. One of them conferred resistance against *P.
hordei-murini*, one against *P. graminis* f.sp. *lolii* and one against
*P. graminis* f.sp. *tritici*. Only the latter has barley-specificity.
As the linkage map for 1H in this analysis purely consisted of AFLP
marker, it was not possible to assign the R-gene or QTLs to the Bin-map.

A new qualitative resistance gene against spot blotch, *Rcs6*, caused by
*Cochliobolus* *sativus*, was localized on chromosome 1H either proximal
on Bin1 or distal on Bin2 by Bilgic *et al*. (2006). They tested the DH
population 'Calcuchima-sib' × 'Bowman-BC' with two different isolates
both on seedlings in the greenhouse and on adult plants in the field.
While one isolate identified the above mentioned resistance gene both in
the seedlings and the adult plants, the other isolate detected different
quantitative resistance loci for the greenhouse compared with the field,
none of them on the position of *Rcs6*.

*Rsp2* and *Rsp3*, originally designated *Sep~2~* and *Sep~3~*, are
barley resistance genes against speckled leaf blotch in barley, caused
by *Septoria passerinii*. These genes were mapped by Zhong *et al*.
(2006) in an F~2:3~ population of the cross \'Foster\' x \'Clho 4780\'
based on seedling tests with a specific isolate. These two genes are
either closely linked or allelic and are localized on chromosome 1H and,
as estimated by the flanking markers, more exactly in Bin3.

Sameri *et al*. (2006) localized QTLs for different agronomic traits in
an RIL population derived from a cross between two Japanese barley
varieties 'Azumamugi' and 'Kanto Nakate Gold'. 'Azumamugi' is an
oriental type barley, while 'Kanto Nakate Gold' belongs to the
occidental type of barley varieties in Japan. The agronomic traits were
evaluated in a field experiment on one location over two years. On
chromosome 1H, one QTL for days to heading was localized near the
position of *Ppd-H2* (photoperiod sensitivity, Bin9/10) and one QTL for
days to heading and days to maturity was detected near the position of
*eam8* ('early maturity', Bin14).

In an F~2:4~ population from a cross between two wild barleys (*H. v.*
ssp. *spontaneum*) from Israel, Vanhala and Stam (2006) localized QTL
for seed dormancy. One of the lines ('Mehola') originates from the
Jordan valley with low humidity and shows high seed dormancy, while the
other line ('Ashkelon') originates from the Mediterranean coast with
relatively high humidity and shows low dormancy. The germination rate
was tested after 0 days, 14 days, 28 days and 42 days of after-ripening
at + 40˚ C. On chromosome 1H, the only QTL where the 'Ashkelon'-allele
prolongated the dormancy was found, while for the four other QTLs, on
chromosomes 2H, 5H, 6H and 7H, 'Mehola' contributed the allele with the
higher dormancy. As the map of 1H was solely based on AFLPs, it was not
possible to assign the position to a Bin.

QTLs for grain yield (Bin11/12), heading date (Bin7), plant height
(Bin14), ear length (Bin9, Bin13), spikelets/spike (Bin8/9), grain/spike
(Bin8/9), spikes/plant (Bin12) and 1000-grain mass (Bin9, Bin11/12) were
detected on chromosome 1H in an advanced-backcross experiment (Li *et
al*. 2006). The wild barley parent was the line 'HS584', and the
recurrent cultivated parent was the variety 'Brenda'. The field trials
were carried out on 2 locations during four years. The map positions of
the marker were based on the 'Igri' × 'Franka' and 'Steptoe' × 'Morex'
SSR maps ((Li *et al*., 2003).

In another advanced-backcross with the wild barley line 'ISR42-8' and
the recurrent cultivated parent 'Scarlett', von Korff *et al*. (2005)
analyzed agronomic traits in a field experiments (four locations during
two years). On 1H, QTLs were found for ears per m² (Bin14), heading date
(Bin13), plant height (Bin13), harvest index (Bin13, Bin14) and yield
(Bin6-8).

**References:**

**Bilgic, H., B. J. Steffenson, and P. M. Hayes. 2006.** Molecular
mapping of loci conferring resistance to different pathotypes of the
spot blotch pathogen in barley. Phytopathology 96(7): 699-708.

**Inukai, T., M. I. Vales, K. Hori, K. Sato, and P. M. Hayes. 2006.**
*RMo1* confers blast resistance in barley and is located within the
complex of resistance genes containing *Mla*, a powdery mildew
resistance gene. Mol. Plant Microbe Interact. 19(9): 1034-1041.

**Jafary, H., L. J. Szabo, and R. E. Niks. 2006.** Innate nonhost
immunity in barley to different heterologous rust fungi is controlled by
sets of resistance genes with different and overlapping specificities.
Mol. Plant Microbe Interact. 19(11): 1270-1279.

**Li, J. Z., X. Q. Huang, F. Heinrichs, M. W. Ganal, and M. S. Roder.
2006.** Analysis of QTLs for yield components, agronomic traits, and
disease resistance in an advanced backcross population of spring barley.
Genome 49(5): 454-466.

**Li, J. Z., T. G. Sjakste, M. S. Röder, and M. W. Ganal. 2003.**
Development and genetic mapping of 127 new microsatellite markers in
barley. Theor. Appl. Genet. 107(6): 1021-1027.

**Rostoks, N., D. Schmierer, S. Mudie, T. Drader, R. Brueggeman, D. G.
Caldwell, R. Waugh, and A. Kleinhofs. 2006.** Barley necrotic locus
*nec1* encodes the cyclic nucleotide-gated ion channel 4 homologous to
the *Arabidopsis* HLM1. Mol. Genet. Genom 275(2): 159-168.

**Sameri, M., K. Takeda, and T. Komatsuda. 2006.** Quantitative trait
loci controlling agronomic traits in recombinant inbred lines from a
cross of oriental- and occidental-type barley cultivars. Breed. Sci.
56(3): 243-252.

**Skinner, J. S., P. Szücs, J. von Zitzewitz, L. Marquez-Cedillo, T.
Filichkin, E. J. Stockinger, M. F. Thomashow, T. H. H. Chen, and P. M.
Hayes. 2006.** Mapping of barley homologs to genes that regulate low
temperature tolerance in Arabidopsis. Theor. Appl. Genet. 112(5):
832-842.

**Szücs, P., I. Karsai, J. von Zitzewitz, K. Meszaros, L. L. D. Cooper,
Y. Q. Gu, T. H. H. Chen, P. M. Hayes, and J. S. Skinner. 2006.**
Positional relationships between photoperiod response QTL and
photoreceptor and vernalization genes in barley. Theor. Appl. Genet.
112(7): 1277-1285.

**Tondelli, A., E. Francia, D. Barabaschi, A. Aprile, J. S. Skinner, E.
J. Stockinger, A. M. Stanca, and N. Pecchioni. 2006.** Mapping
regulatory genes as candidates for cold and drought stress tolerance in
barley. Theor. Appl. Genet. 112(3): 445-454.

**Vanhala, T. K. and P. Stam. 2006.** Quantitative trait loci for seed
dormancy in wild barley (*Hordeum spontaneum* c. Koch). Genet. Resour.
Crop. Evol. 53(5): 1013-1019.

**von Korff, M., H. Wang, and J. Léon. 2005.** AB-QTL analysis in spring
barley. I. Detection of resistance genes against powdery mildew, leaf
rust and scald introgressed from wild barley. Theor. Appl. Genet.
111(3): 583-590.

**Zhong, S. B., H. Toubia-Rahme, B. J. Steffenson, and K. P. Smith.
2006.** Molecular mapping and marker-assisted selection of genes for
septoria speckled leaf blotch resistance in barley. Phytopathology
96(9): 993-999.

**Coordinator's report: Chromosome 2H (2)**

# J.D. Franckowiak

**Hermitage Research Station**

**Queensland Department of Primary Industries and Fisheries**

**Warwick, Queensland 4370, Australia**

**[e-mail: jerome.franckowiak@dpi.qld.gpv.au]{.underline}**

Komatsuda *et al*. (2007) cloned the six-rowed spike 1 (*vrs1*) gene
located on chromosome 2HL of barley. Expression of the *Vrs1* was
strictly localized in the lateral-spikelet primordia of immature spikes
and suggests that the VRS1 protein suppresses development of lateral
spikelets. Phylogenetic analysis of the six-rowed cultivars and mutants
demonstrated that six-rowed spike trait originated repeatedly from
two-rowed barley, at least three different origins among domesticated
accessions. Also, the DNA sequence defects in many of *vrs1* mutants
held in the Nordic Gene Bank were identified.

When the DNA sequence of *vrs1* was determined, Pourkheirandish *et al*.
(2007) found that the region around the *vrs1* locus was collinear with
rice chromosome 4. However, the rice orthologue for the *vrs1* sequence
was found on rice chromosome 7. The authors speculated that a
transposition of the chromosomal segment *Vrs1* to chromosome 2H
occurred during the evolution of barley. Pourkheirandish *et al*. (2007)
also reported that the *vrs1* locus is a region of suppressed
recombination based on the study of more than 13,000 gametes.

Řepková *et al*. (2006) reported on the mapping of four new sources of
resistance to powdery mildew, caused by *Blumeria graminis* f. sp.
*hordei*, that were identified in accessions of wild barley, *Hordeum
vulgare* ssp. s*pontaneum*. Accession PI 466197 was found to have two
dominant resistance genes. One is an allelic at the *mla* locus and the
other was located on chromosome 2HS based on a highly significant
linkage with molecular marker Bmac0134.

Dahleen and Franckowiak (2006) found that *cer-zt* locus is located on
chromosome 2HS based on linkage to molecular marker Bmac0134 in bin
2H-1. The *cert-zt.389* mutant has very little surface wax on the spike
(Lundqvist and Franckowiak, 1997), but little effect on other agronomic
traits except a slightly increased number of kernels per spike (Dahleen
and Franckowiak, 2006).

Based on the analysis of 134 recombinant chromosome substitution lines
(RCLs) from the BC~3~ generation of the backcross of wild barley line
(OUH602) into 'Haurna Nijo', Hori *et al*. (2005) found that QTLs for
short spike and lax spike are on chromosome 2HL near the closed
flowering (cleistogamy, *cly1*/*Cly*2) locus of Haurna Nijo. In a
previous paper, Hori *et al*. (2003) reported that these QTLs plus one
for short culm were observed in a population of doubled-haploid lines
from a Haurna Nijo/OUH602 cross.

Using recombinant inbred lines, Yun *et al*. (2005) found a QTL for
resistance to Septoria speckled leaf blotch (SSLB, caused by *Septoria
passerinii* Sacc.) from *H. vulgare* subsp. *spontaneum*, located in
bins 7 to 11 of chromosome 2H. They examined a recombinant inbred line
(RIL) population developed from a cross between wild barley accession
OUH602 and the two-rowed malting cultivar 'Harrington' for reaction to
SSLB. About 40% of the variation in response to SSLB was explained by
the QTL on 2H, named QTL Rsp-2H-7-ll. The mapped disease resistances
were validated using an advanced backcross population (BC~2~F~6:8~) from
the same donor parent, but having two more backcrosses to Harrington
(Yun *et al*., 2006).

A QTL regulating synthesis of cell wall (1,3;1,4)-beta-D-glucans was
located between the markers *Adh8* bin 6 and ABG019 bin 7 with the peak
closer to ABG019 on 2H (Burton *et al*., 2006). The cellulose
synthase-like (*CslF*) gene cluster in cereals was identified as
candidates responsible for mediating cell wall (1,3;1,4)- ß -D-glucan
synthesis using of rice synteny and by transforming Arabidopsis (Burton
et al., 2006). The research was based on the map location of a major QTL
for (1,3;1,4)-ß-D-glucan content of un-germinated barley grains on 2H.
This report is believed the first example of a map-based cloning of a
QTL in barley.

Korff *et al*. (2006) reported on a large number of QTLs for agronomic
traits detected in doubled-haploid lines from the second backcross of
'Scarlett' backcrossed to *Hordeum vulgare* ssp. *spontaneum* accession
ISR42-8. Using a population 301 BC~2~DH in eight environments, they
reported detection of 86 QTLs for nine agronomic traits. The QTLs having
large effects that were associated with chromosome 2H included: ears/m2,
days to head (*Eam1* or *Ppd-H1*), plant height (*sdw1* from Scarlett),
and yield.

Yin *et al*. (2005) confirmed that a QTL having an important effect on
preflowering duration in the 'Apex'/'Prisma' population of 94
recombinant inbred lines (RILs) was located on the long arm of
chromosome 2H. The other QTL having a large effect was located on
chromosome 3H at the same position as the *sdw1* gene from Prisma.

Dragan *et al*. (2007) located two members of the nicotianamine synthase
(NAS) family of genes on the short arm of chromosome 2H (2HS).
Nicotianamine is involved chelation of iron and other heavy metals and
their transport in the plant.

The number of molecular markers located on chromosome 2H has been
increased by several studies. Beaubien and Smith (2006) placed 7 of the
60 new mapped SSR markers on 2H at bin positions that previously had
been identified as being poorly covered by SSR markers currently
available. Stein *et al*. (2007) published an expressed sequence tag
(EST)-based map for barley based 200 anchor markers from three
previously published maps. The map contained 1,055 loci and a map size
of 1,118.3 cM. The map for 2H contained 179 EST loci and a map length of
165.1 cM. Using barley-wheat addition lines and the Barley1 Affymetrix
GeneChip probe array, Cho *et al*. (2006) associated 1,787 of 4,104
transcript accumulation patterns detected in Betzes, but not Chinese
Spring, with specific barley chromosomes. Of these 271 were associated
with the 2H addition line of Chinese Spring.

Takahashi *et al*. (2006) mapped in barley miniature inverted-repeat
transposable elements (MITEs), which represent a large superfamily of
transposons that is moderately to highly repetitive and frequently found
near or within plant genes. To elucidate the organization of MITEs in
the barley genome, MITEs were integrated into the genetic map of barley
using 93 doubled haploid lines from a Haruna Nijo by *H. vulgare* ssp.
*spontaneum* accession OUH602 cross. They described the use of MITEs in
amplified fragment length polymorphism (AFLP) mapping and demonstrate
their superiority over conventional AFLP mapping. A total of 214 loci
covered a total map distance of 1,165 cM, and 39 were placed on 2H.

**References:**

**Beaubien, K.A., and K. P. Smith. 2006.** New SSR markers for barley
derived from the EST database. Barley Genet. Newsl. 36:30-36.

**Burton, R.A., S.M. Wilson, M. Hrmova, A.J. Harvey, N.J. Shirley, A.
Medhurst, B.A. Stone, E.J. Newbigin, A. Bacic, and G.B. Fincher. 2006.**
Cellulose synthase-like *CslF* genes mediate the synthesis of cell wall
(1,3;1,4)-beta-D-glucans. Science 311:1940-1943.

**Cho, S., D.F. Garvin, and G.J. Muehlbauer. 2006.** Transcriptome
analysis and physical mapping of barley genes in wheat--barley
chromosome addition lines. Genetics 172:1277-1285.

**Dahleen, L.S. and J.D. Franckowiak. 2006.** SSR linkages to eight
additional morphological marker traits. Barley Genet. Newsl. 36:12-16.

**[Dragan
Perovic](http://www.springerlink.com/content/?Author=Dragan+Perovic),
D., [P.
Tiffin](http://www.springerlink.com/content/?Author=Peter+Tiffin), [D.
Douchkov](http://www.springerlink.com/content/?Author=Dimitar+Douchkov),
[H.
Bäumlein](http://www.springerlink.com/content/?Author=Helmut+Bäumlein),
and [A.
Graner](http://www.springerlink.com/content/?Author=Andreas+Graner).
2007.** [An integrated approach for the comparative analysis of a
multigene family: The nicotianamine synthase genes of
barley](http://www.springerlink.com/content/7877543216427402/?p=8796e4babd3b4c4f9d69b0bb7673d59d&pi=10).
[Funct. Integr. Genomics](http://www.springerlink.com/content/105695/)
7:169-179.

**Hori, K., T. Kobayashi, A. Shimizu, K. Sato, K. Takeda, and S.
Kawasaki.** 2003. Efficient construction of high-density linkage map and
its application to QTL analysis in barley. Theor. Appl. Genet.
107:806-813.

**Hori, K., K. Sato, N. Nankaku, and K. Takeda. 2005.** [QTL analysis in
recombinant chromosome substitution lines and doubled haploid lines
derived from a cross between *Hordeum vulgare* ssp. *vulgare* and
*Hordeum vulgare* ssp.
*spontaneum*](http://www.springerlink.com/content/737w48485qu28817/?p=903a4ef521484a35b1f9d7eb7e47ee22&pi=3).
Mol. Breeding 16(4):295-311.

**Komatsuda, T., M. Pourkheirandish, C. He, P. Azhaguvel, H. Kanamori,
D. Perovic, N. Stein, A. Graner, T. Wicker, A. Tagiri, U. Lundqvist, T.
Fujimura, M. Matsuoka, T. Matsumoto, and M. Yano. 2007.** Six-rowed
barley originated from a mutation in a homeodomain-leucine zipper
I-class homeobox gene. PNAS 104:1424-1429.

**von [Korff](http://www.springerlink.com/content/?Author=M.+von+Korff),
M., [H. Wang](http://www.springerlink.com/content/?Author=H.+Wang), [J.
Léon](http://www.springerlink.com/content/?Author=J.+Léon), and [K.
Pillen](http://www.springerlink.com/content/?Author=K.+Pillen). 2006.**
[AB-QTL analysis in spring barley: II. Detection of favourable exotic
alleles for agronomic traits introgressed from wild barley (*H. vulgare*
ssp.
*spontaneum*)](http://www.springerlink.com/content/y331x8755051h71j/?p=8e7dfcb66bd04c5f9c568364a28f27c8&pi=2).
Theor. Appl. Genet. 112:1221-1231.

**Lundqvist, U., and J.D. Franckowiak. 1997.** BGS 437, Eceriferum-zt,
*cer-zt*. Barley Genet Newsl. 26:389

**Pourkheirandish, M., T. Wicker, N. Stein, T. Fujimura, and T.
Komatsuda. 2007.** Analysis of the barley chromosome 2 region containing
the six-rowed spike gene *vrs1* reveals a breakdown of the rice-barley
micro collinearity by a transposition. Theor. Appl. Genet.
114:1357-1365.

**Řepková, J., A. Dreiseitl, P. Lízal, Z. Kyjovská, K. Teturová, R.
Psotková, and A. Jahoor. 2006.** Identification of resistance genes
against powdery mildew in four accessions of *Hordeum vulgare* ssp.
s*pontaneum*. Euphytica 151:23-30.

**Stein, N., M. Prasad, U. Scholz, T. Thiel, H. Zhang, M. Wolf, R. Kota,
R.K. Varshney, D. Perovic, I. Grosse, and A. Graner. 2007.** A
1,000-loci transcript map of the barley genome: new anchoring points for
integrative grass genomic. Theor. Appl. Genet. 114:823-839.

**[Takahashi](javascript:void(0);), H., [H. Akagi](javascript:void(0);),
[K. Mori](javascript:void(0);), [K. Sato](javascript:void(0);), and [K.
Takeda](javascript:void(0);). 2006.** Genomic distribution of MITEs in
barley determined by MITE-AFLP mapping. Genome 49:1616- 1618.

**Yin, X., P.C. Struik, F.A. van Eeuwijk, P. Stam, and J. Tang. 2005.**
[QTL analysis and QTL-based prediction of flowering phenology in
recombinant inbred lines of
barley](http://proquest.umi.com/pqdweb?index=18&did=818458691&SrchMode=3&sid=2&Fmt=10&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1183339531&clientId=62902&aid=1)
J. Exp. Bot. 56 (413):967-976.

**Yun, S.J., L. Gyenis, E. Bossolini, P.M. Hayes, I. Matus, K.P. Smith,
B.J. Steffenson, R. Tuberosa, and G.J. Muehlbauer. 2006.** Validation of
quantitative trait loci for multiple disease resistance in barley using
advanced backcross lines developed with a wild barley. Crop Sci.
46:1179-1186.

**Yun, S.J., L. Gyenis, P.M. Hayes, I. Matus, K.P. Smith, B.J.
Steffenson, and G.J. Muehlbauer. 2005.** Quantitative trait loci for
multiple disease resistance in wild barley. Crop Sci. 45:2563-2572.

**Coordinator's Report: Barley Chromosome 3H**

**L. Ramsay**

**Genetics Programme**

**Scottish Crop Research Institute**

**Invergowrie, Dundee, DD2 5DA, Scotland, UK.**

**[e-mail: Luke.Ramsay@scri.ac.uk]{.underline}**

Over the last year there have been a number of publications reporting
the mapping of genes and QTL on barley chromosome 3H. One of the
highlights of this reporting period was the genetic mapping of over 1000
genes, including 179 on 3H, by Stein *et al.* (2007). This worked
confirmed the syntenic relationship of 3H to rice chromosome 1 and
importantly put the detailed EST information underlying this and
previous reports in the public domain. This includes the details of
which genes are represented by the EST derived microsatellites reported
by Varshney *et al.* (2006) that included 35 that map to 3H. Another
report of EST derived microsatellites with the associated EST
information, including 11 on 3H, was that of Beaubien and Smith (2006).

Another important general mapping paper was the development of a
consensus map derived from DArT marker loci (Wenzl *et al*, 2006) that
opens up the possibility of using loci derived from this technology as
proxies for more expensive genic markers. Also of importance is the
detailed consensus map presented by Marcel *et al.* (2007) which brings
together standard AFLP, microsatellite and RFLP loci and that will allow
additional alignment of past work with the positions of genic loci.

A range of QTL on chromosome 3H were again reported this year. In a RIL
population derived from a cross between Azumamugi and Kanto Nakate Gold
studied by Sameri *et al.* (2006) QTL were found on 3H for a range of
agronomic characters including plant height, spike length and awn
length. The position of the QTL found indicates that they are due to the
segregation of *uzu* in the population. Li *et al*. (2006) reported the
positions of QTL for a range of agronomic traits using recombinant
chromosome substation lines derived from a *Hordeum* *vulgare* subsp.
*vulgare* (cltv. Brenda) by *Hordeum* *vulgare* subsp. *spontaneum*
(accession HS584) cross to delineate association with genomic regions.
The QTL found on 3H included those for yield and components such as
spikelet no. per spike, grain no. per spike, thousand-grain mass as well
as other traits such as heading date, plant height, ear length, leaf
length and leaf area. A QTL for resistance on 3H was also found to leaf
rust in two trials which may relate to the two QTL for leaf rust
resistance found on chromosome 3H in a consensus map by Marcel *et al.*
(2007) in a summary of work on six mapping populations. One of the
populations used in the construction of this consensus map, L94 x Vada,
was also tested for mildew and scald resistance and a novel powdery
mildew resistance QTL designated Rbgq2 was detected on 3H which did not
map to a region where a major gene for powdery mildew has previously
been reported (Shtaya *et al.* 2006). Another of the populations
included in the report of Marcel *et al.* 2007 was that derived from a
cross between an experimental line SusPrit and Vada to study the
inheritance of non-host immunity to rusts (Jafary *et al*. 2006). This
work found three QTL on 3H associated with host and non-host resistance
to *Puccinia* spp. Other disease QTL reported on 3H included the
improved resolution of spot blotch resistance QTL on the Calicuchima-sib
/ Bowman BC population by Bilgic *et al.* (2006) and a scald resistance
QTL on the long arm of 3H identified using a partial map of a doubled
haploid population derived from a Mundah/Keel cross (Cheong *et al.*,
2006).

**References:**

**Beaubien, K.A. and K.P. Smith. 2006** New SSR markers for barley
derived from the EST database. Barley Genetics Newsletter 36: 30-43.

**Bilgic, H., B.J. Steffenson, and P.M. Hayes. 2006.** Molecular mapping
of loci conferring resistance to different pathotypes of the spot blotch
pathogen in barley. Phytopathology 96: 699-708.

**Cheong, J., K. Williams, and H. Wallwork, 2006.** The identification
of QTLs for adult plant resistance to leaf scald in barley. Australian
Journal of Agricultural Research 57: 961-965.

**Jafary, H., L.J. Szabo, and R.E. Niks, 2006.** Innate nonhost immunity
in barley to different heterologous rust fungi is controlled by sets of
resistance genes with different and overlapping specificities. Molecular
Plant-Microbe Interactions 19: 1270-1279.

**Li, J.Z., X.Q. Huang, F. Heinrichs, M.W. Ganal, and M.S. Roder,
2006.** Analysis of QTLs for yield components, agronomic traits, and
disease resistance in an advanced backcross population of spring barley.
Genome 49: 454-466.

**Marcel, T.C., R.K. Varshney, M. Barbieri, H. Jafary, M.J.D. de Kock,
A. Graner and R.E. Niks, 2007.** A high-density consensus map of barley
to compare the distribution of QTLs for partial resistance to *Puccinina
hordei* and of defence gene homologues. Theor Appl. Genet 114: 487-500.

**Samuri, M., K. Takeda and Komatsuda, T. 2006.** Quantitative trait
loci controlling agronomic traits in recombinant inbred lines from a
cross of oriental- and occidental-type barley cultivars. Breeding
Science 56: 243-252.

**Shtaya, M.J.Y., T.C. Marcel, J.C. Sillero, R.E. Niks, and D. Rubiales,
2006.** Identification of QTLs for powdery mildew and scald resistance
in barley. Euphytica 151: 421-429.

**Stein, N., M. Prassa, U. Scholz, T. Thiel, H. Zhang, M. Wolf, R. Kota,
R.K. Varshney, D. Perovic, I. Grosse and A. Graner, 2007**. A 1,000-loci
transcript map of the barley genome: new anchoring points for
integrative grass genomics. Theor Appl Genet on-line preprint DOI
10.1007/s00122-006-0480-2

**Varshney, R.K., I. Grosse, U. Hahnel, R. Siefken, M. Prasad, N. Stein,
P. Langridge, L. Altschmied, and A. Graner, 2006.** Genetic mapping and
BAC assignment of EST-derived SSR markers shows non-uniform distribution
of genes in the barley genome. Theor Appl Genet 113: 239-250.

**Wenzl, P., H.B. Li, J. Carling, M.X. Zhou, H. Raman, E. Paul, P.
Hearnden, C. Maier, L. Xia, V. Caig, J. Ovesna, M. Cakir, D. Poulsen,
J.P. Wang, R. Raman, K.P. Smith, G.J. Muehlbauer, K.J. Chalmers, A.
Kleinhofs, E. Huttner, and A. Kilian, 2006.** A high-density consensus
map of barley linking DArT markers to SSR, RFLP and STS loci and
agricultural traits. BMC Genomics 7:206.

**Coordinator's Report: Chromosome 4H**

**Arnis Druka**

**Genetics Programme**

**Scottish Crop Research Institute**

> **Invergowrie, Dundee, DD2 5DA, Scotland, UK.**

**e-mail: <adruka@scri.sari.ac.uk>**

Several papers that relate to the genes on chromosome 4H have been
published in 2006 - 2007. At least four of them combine mRNA abundance
analyses with phenotypic trait genetic analyses clearly showing added
value of such approach (Malatrasi *et al.,* 2006; Zhang *et al.,* 2006;
Wang *et al*., 2007 and Walia *et al.*, 2007).

The *HvMATE* gene, encoding a multidrug and toxic compound extrusion
protein has been identified as a candidate controlling aluminium (Al)
tolerance in barley. The gene itself was found not to be polymorphic
between Al-tolerant and sensitive cultivars, but it accumulates mRNA 30
times more in the Al tolerant cultivar. *HvMATE* mRNA accumulation was
measured in the F(2:3) families and was found significantly correlated
with the Al tolerance and Al-activated citrate efflux phenotypes that
have been mapped on the long arm of chromosome 4H (Wang *et al.,* 2007).

A different study addressed the salt tolerance in barley by analysing
single feature polymorphisms (SFPs) and an oligonucleotide pool assay
for single nucleotide polymorphisms (SNPs) in the salt tolerant cultivar
Golden Promise and intolerant cultivar Maythorpe. Golden Promise has
been generated by inducing mutation in the cultivar Maythorpe. The
transcriptome analysis indicates that the response of the two genotypes
to the salinity stress is quite different.. This study identified 3
haplotype blocks spanning 6.4 cM on chromosome 1H, 23.7 cM on chromosome
4H and 3.0 cM on 5H suggesting that Golden Promise is not isogenic
(Walia *et al*., 2007).

A gene encoding the branched-chain amino acid aminotransferase
(*HvBCAT-1*) that mapped on chromosome 4H, was identified by using
differential mRNA display applied to ABA, drought and cold treated
barley seedling shoots. Transcript levels of *Hvbcat-1* increased in
response to drought stress. The complementation of a yeast double
knockout strain revealed that *HvBCAT-1* can function as the
mitochondrial (catabolic) *BCATs* *in vivo*. This allowed to put forward
the hypothesis, that under drought stress conditions, one of the
detoxification mechanisms could be associated with degradation of the
branched-chain amino acids (Malatrasi *et al*., 2006).

Zhang *et al*. (2006) have reported a novel locus that is required for
*Rpg1* gene mediated resistance to the stem rust (*Puccinia graminis f.
sp. tritici*) fungus. It was identified by inducing the irradiation
mutations in the resistant barley cultivar and selecting for susceptible
individuals in the M2 progeny. *Rpg1* gene in one such susceptible
mutant plants was found to be intact and the following mutation mapping
identified a locus on chromosome 4H, that was named *Rpr1* (*Required
for P. graminis resistance*). Several candidate genes or novel markers
for this locus were identified by using large scale parallel transcript
profiling approach.

Other papers that related to chromosome 4H were describing either
characterization and mapping gene families and the candidate genes for
certain QTLs (Brueggeman *et al*., 2006; Skinner *et al*., 2006) or
mapping novel QTLs (Friesen *et al*., 2006; Richardson *et al*., 2006;
Yan and Chen 2006; von Korff *et al*., 2006).

Thus, Brueggeman *et al*. (2006) reported mapping of members of the
serine/threonine kinase-like protein family that encode at least one
predicted catalytically active kinase domain. One of them was localized
to chromosome 4H. In a different study, allelic nature and map locations
of barley homologs to three classes of Arabidopsis low temperature
regulatory genes-*CBFs*, *ICE1*, and *ZAT12* were investigated for
associations with the LT tolerance QTLs. In the same study, phenotyping
of the Dicktoo x Morex (DxM) mapping population under controlled
freezing conditions identified three new low temperature tolerance (LT)
QTLs on 1H-L, 4H-S, and 4H-L in addition to the previously reported 5H-L
Fr-H1 QTL. (Skinner *et al*., 2006).

Barley interaction with the net blotch fungi, *Pyrenophora teres f.
teres* (net-type net blotch (NTNB)) and *Pyrenophora teres f. maculata*
(spot-type net blotch (STNB)) was studied using a doubled-haploid
population derived from the lines SM89010 and Q21861. Major QTLs for
NTNB and STNB resistance were located on chromosomes 6H and 4H,
respectively (Friesen *et al*., 2006).

Barley and the stripe rust fungus (*Puccinia striiformis f. sp. hordei*)
interaction phenotypes, such as latency period, infection efficiency,
lesion size and pustule density were mapped using i-BISON lines
(intermediate barley near-isogenic lines). The (i-BISON) lines
represented disease resistance QTL combined in one-, two-, and three-way
combinations in a susceptible background. The 4H QTL allele had the
largest effect followed by the alleles on chromosomes1H and 5H
(Richardson *et al*., 2006).

In a different study Yan and Chen (2006) reported population of 182
recombinant inbred lines (RILs) (F8) derived from cultivars Steptoe and
GZ that was generated to map the resistance to two barley stripe rust
fungus strains on the long arm of barley chromosome 4H.

The BC2DH population derived from a cross between the spring barley
cultivar Scarlett and the wild barley accession ISR42-8 (*Hordeum
vulgare ssp. spontaneum*) was developed to evaluate nine agronomic
traits. Favourable ISR42-8 alleles were detected for the yield-related
traits that have QTLs on the long arm of chromosome 4H (von Korff *et
al*., 2006).

**References:**

**Brueggeman R. T. Drader, and A. Kleinhofs 2006.** The barley
serine/threonine kinase gene Rpg1 providing resistance to stem rust
belongs to a gene family with five other members encoding kinase
domains. Theor. Appl. Genet. 113(6):1147-1158.

**Friesen T.L., J.D. Faris, Z. Lai, and B.J. Steffenson. 2006**.
Identification and chromosomal location of major genes for resistance to
*Pyrenophora teres* in a doubled-haploid barley population. Genome
49(7):855-859.

**Malatrasi M., M. Corradi, J.T. Svensson, T.J. Close, M. Gulli, and N.
Marmiroli 2006.** A branched-chain amino acid aminotransferase gene
isolated from *Hordeum vulgare* is differentially regulated by drought
stress. Theor. Appl. Genet. 113(6):965-976.

**Richardson K.L., M.I. Vales, J.G. Kling, C.C. Mundt, and P.M. Hayes.
2006.** Pyramiding and dissecting disease resistance QTL to barley
stripe rust. Theor. Appl. Genet. 113(3):485-495.

**Skinner J.S., P. Szucs, J. von Zitzewitz, L. Marquez-Cedillo, T.
Filichkin, E.J. Stockinger, M.F. Thomashow, T.H. Chen, and P.M. Hayes.
2006**. Mapping of barley homologs to genes that regulate low
temperature tolerance in *Arabidopsis*. Theor. Appl. Genet.
112(5):832-842.

**von Korff M., H. Wang, J. Léon, and K. Pillen. 2006.** AB-QTL analysis
in spring barley: II. Detection of favourable exotic alleles for
agronomic traits introgressed from wild barley (*H. vulgare ssp.
spontaneum*). Theor. Appl. Genet. 112(7):1221-1231.

**Walia H., C. Wilson, P. Condamine, A.M. Ismail, J. Xu, X. Cui, and
T.J. Close. 2007.** Array-based genotyping and expression analysis of
barley cv. Maythorpe and Golden Promise. BMC Genomics. Mar 30; 8:87.

**Wang J., H. Raman, M. Zhou, P.R. Ryan, E. Delhaize, D.M. Hebb, N.
Coombes, and N. Mendham. 2007**. High-resolution mapping of the *Alp*
locus and identification of a candidate gene *HvMATE* controlling
aluminium tolerance in barley (*Hordeum vulgare L.*). Theor. Appl.
Genet. Jun 6 \[Epub ahead of print\].

**Yan G.P. and X.M. Chen.** Molecular mapping of a recessive gene for
resistance to stripe rust in barley. Theor. Appl. Genet. 2006
113(3):529-537.

**Zhang L., T. Fetch, J. Nirmala, D. Schmierer, R. Brueggeman, B.
Steffenson, and A. Kleinhofs. 2006**. *Rpr1*, a gene required for
*Rpg1*-dependent resistance to stem rust in barley. Theor. Appl. Genet.
113(5):847-855.

Coordinator's Report: Chromosome 5H

**George Fedak**

**Eastern Cereals and Oilseeds Research Centre**

**Agriculture and Agri-Food Canada**

**Ottawa ON K1A 0C6**

**e-mail: [fedakga@agr.gc.ca]{.underline}**

The grain hardness locus (Ha) of barley consists of a cluster of genes
located on the short arm of 5H designated as Hina, Hinb-1, Hinb-2 and
GSP. Eighty diverse barley genotypes were screened for kernel hardness,
ruminant digestibility and haplotypes of the four alleles. The highest
level of genetic variation was obtained with GSP followed by Hina,
Hinb-2. Hina was significantly related to grain hardness while Hinb-1
and Hinb-2 were significantly associated with dry water digestibility.
(Turuspekov *et al*., 2007).

Using the Nure (winter) x Tremois (spring) mapping population, two low
temperature QTL were located on the long arm of chromosome 5H. *FrHi*
was located in a distal position and *Fr-H2* in a proximal location. The
location of the latter coincided with the location of a QTL regulating
the accumulation of two COR proteins; COR14b and TMC-Ap3. Six barley
genes for the CBF transcription factor have been mapped in a single
cluster in this region and they represent candidate genes for Fr-H2.
(Francia *et.al*., 2007)

In a related study, Lombda phage libraries were constructed from 2
spring (Morex and Tremois) and two winter (Dicktos, Nure) cultivars.
Clones containing CBF genes were sequenced. It was found that the winter
varieties have a large duplication at the *Fr-H2* gene resulting in an
increased number of CBF genes at this locus. The spring barley Tremois,
however, has a significant deletion at this locus. This suggests that
the relative numbers of CBF in the cluster contributes to different
levels of winterhardiness (Knox *et.al*.,2007).

**References:**

**Francia, E., D. Baraboschi, A. Tondelli, G. Laido, A.M. Stanca, E.
Stockinger and N. Pecchioni, 2007.** Fine mapping of a Hv CBF gene
cluster, at Fr-H2, a QTL controlling frost resistance in barley. PAG -
XV Poster 331. Plant and Animal Genomes XV Conference, Jan 13-17, 2007.
San Diego CA.

**Knox, A.K., H. Chang . and E.I. Stockinger, 2007.** Comparative
Sequence analysis of CBF genes at the Fr-H2 locus in four barley
cultivars. PAG -- XV Poster 321. Plant and Animal Genomes XV Conference,
Jan 13-17, 2007. San Diego CA.

**Turuspekov Y., B. Beecher, Y. Darlington, J. Bowman, T. Blake and M.
Gioux. 2007.**Genetic variation of hardness locus in barley. PAG-XV
Poster 329. Plant and Animal Genomes XV Conference, Jan 13-17, 2007, San
Diego CA.

**Coordinator's Report: Chromosome 7H**

# Lynn S. Dahleen

**USDA-Agricultural Research Service**

**Fargo, ND 58105, USA**

**e-mail: [DAHLEEN@fargo.ars.usda.gov]{.underline}**

Barley gene mapping in 2006 showed a greater emphasis on using candidate
gene approaches in addition to standard qualitative and quantitative
trait mapping. Increased use of public EST and BAC libraries was
evident, providing tools to better understand the barley genome.

Efforts to map morphological genes have continued. Roder *et al.* (2006)
mapped the shrunken endosperm gene *seg8* to a 4.6 cM interval near the
centromere of chromosome 7H, while Taketa *et al.* (2006) developed a
fine map of the naked caryopsis *nud* locus, placing it in a 0.66 cM
region. Rostoks *et al*. (2006) found that the barley homolog of the
*Arabidopsis* *HLM1* gene corresponded to the *nec1* locus on chromosome
7H. Allelic variation was uncovered at the locus that causes necrotic
spotting of *nec1* plants. Rossini *et al.* (2006) examined candidate
rice genes in regions syntenous with markers linked to various barley
morphological mutants. On chromosome 7H they found *brh1* candidate
genes on rice chromosome 6 and candidates for *suKF-76, suKE-74*
(suppressors of Hooded) and *sld4* on rice chromosomes 6 and 8. The low
resolution of the barley maps in this region resulted in selection of
rather large rice regions and numerous candidate genes. Yan *et al*.
(2006) identified the *AtFT* flowering locus as an ortholog of the
barley and wheat vernalization gene *VRN3*. The barley gene *VRN-H3* was
located on the short arm of chromosome 7H, not chromosome 1H as
previously thought based on loose linkage with *BLP*. Szucs *et al*.
(2006) mapped genes for photoreceptor gene families and vernalization
regulation, and compared their locations to QTL for photoperiod
response. The barley ortholog to a wheat flowering repressor, *HvVRT-2*
mapped to the short arm of chromosome 7H. This locus coincided with a
photoperiod QTL with small effects mapped in the Dicktoo x Morex
population. Tondelli *et al*. (2006), using a similar approach, mapped
candidate genes for cold or drought response based on sequences
identified in other plants. Two orthologs of *Arabidopsis* genes
(*AtFRY1* and *AtICE1*) that have a prominent role in cold acclimation
were identified on chromosome 7H.

QTL analyses for a variety of traits were reported this year. Chloupek
*et al.* (2006) mapped root system size traits in a population
segregating for two semidwarf genes, *sdw1* and *ari-e.GP*. On
chromosome 7H, they identified a region associated with height, and
another region associated with harvest index, plant weight, root system
size at grain filling and total root system size. Advanced backcross QTL
analysis continued, with von Korff *et* *al*. (2006) detecting favorable
alleles from wild barley in crosses with Scarlett. Out of the 86 QTL
identified for 9 traits, the *H. spontaneum* alleles improved
performance for 31. QTL for height, heading date, harvest index, lodging
at flowering, vegetative dry biomass, thousand grain weight, brittleness
and yield were located on chromosome 7H. Li *et al.* (2006), in a
similar study of a wild barley x Brenda advanced backcross, found 100
QTL. Chromosome 7H QTL included yield, heading date, height, ear length,
spikelets per spike, seed per spike, spikes per plant, thousand grain
weight, leaf length, and leaf area loci. Yun *et al.* (2006) also used
advanced backcross lines from a cross of *H. spontaneum* with Harrington
to validate QTLs for disease resistance loci. A QTL for spot blotch
resistance previously identified in a RIL population was confirmed to be
located on chromosome 7H.

Additional disease resistance genes were located in several studies.
Cheong *et al*. (2006) located a QTL for adult plant resistance to leaf
scald using two populations. One of these QTL was located on the short
arm of chromosome 7H. Rossi *et al.* (2006) located QTL for barley strip
rust and leaf rust resistance plus a powdery mildew resistance QTL on
chromosome 7H. Jafary *et al*. (2006) located genes involved in nonhost
immunity to rust pathogens, including four QTL on chromosome 7H
controlling reactions to seven rust species. Brueggeman *et al.* (2006)
identified five additional members of the *Rpg1* gene family, including
one that is closely linked to *Rpg1* on chromosome 7H.

Kilian *et al*. (2006) examine haplotype structure at seven loci,
including the *Adh3* and *Waxy* loci on chromosome 7H, to compare
sequence diversity between 20 domesticated and 25 wild barleys. As
expected, more haplotypes were identified in the wild barley than the
domesticated barley. At *Adh3*, wild barley showed 15 haplotypes while
domesticated barley had three and at *Waxy*, the wilds had 17 haplotypes
compared to 4 in the domesticated barley. This diversity was also
evident in nucleotide sequence, with more polymorphic sites in the wild
barley than in the domesticated barley. Pickering *et al.* (2006)
examined associations between chromosomes in two *H. vulgare* x *H.
bulbosum* hybrids. Chromosome 7HS-7H^b^S associations were higher than
the average for other chromosome arms in both hybrids examined.

**References.**

**Brueggeman R, T Drader and A Kleinhofs. 2006.** The barley
serine/threonine kinase gene *Rpg1* providing resistance to stem rust
belongs to a gene family with five other members encoding kinase
domains. Theor Appl Genet 113:1147-1158.

**Cheong J, K Williams and H Wallwork. 2006.** The identification of
QTLs for adult plant resistance to leaf scald in barley. Austral J Agric
Res 57:961-965.

**Chloupek O, BP Forster and WTB Thomas. 2006.** The effect of
semi-dwarf genes on root system size in field-grown barley. Theor Appl
Genet 112:779-786.

**Jafary H, LJ Szabo and RE Niks. 2006.** Innate nonhost immunity in
barley to different heterologous rust fungi is controlled by sets of
resistance genes with different and overlapping specificities. MPMI
19:1270-1279.

**Kilian B, H Ozkan, J Kohl, A von Haeseler, F Borale, O Deusch, A
Brandolini, C Yucel, W Martin and F Salamini. 2006.** Haplotype
structure at seven barley genes: relevance to gene pool bottlenecks,
phylogeny of ear type and site of barley domestication. Mol Gen Genomics
276:230-241.

**Korff M von, H Wang, J Leon and K Pillen. 2006.** AB-QTL analysis in
spring barley: II. Detection of favourable exotic alleles for agronomic
traits introgressed from wild barley (*H. vulgare* ssp. *spontaneum*).
Theor Appl Genet 112:1221-1231.

**Li JZ, XQ Huang, F Heinrichs, MW Ganal and MS Roder. 2006.** Analysis
of QTLs for yield components, agronomic traits, and disease resistance
in an advanced backcross population of spring barley. Genome 49:454-466.

**Pickering R, S Klatte and RC Butler. 2006.** Identification of all
chromosome arms and their involvement in meiotic homoeologous
associations at metaphase 1 in 2 *Hordeum vulgare* L. x *Hordeum
bulbosum* L. hybrids. Genome 49:73-78.

**Roder MS, C Kaiser and W Weschke. 2006.** Molecular mapping of the
shrunken endosperm genes *seg8* and *sex1* in barley (*Hordeum vulgare*
L.). Genome 49:1209-1214.

**Rossi C, A Cuesta-Marcos, I Vales, L Gomez-Pando, G Orjeda, R Wise, K
Sato, K Hori, F Capettini, H Vivar, X Chen and P Hayes. 2006.** Mapping
multiple disease resistance genes using a barley mapping population
evaluated in Peru, Mexico, and the USA. Mol Breed 18:355-366.

**Rossini L, A Vecchietti, L Nicoloso, N Stein, S Franzago, F Salamini
and C Pozzi. 2006.** Candidate genes for barley mutants involved in
plant architecture: an *in silico* approach. Theor Appl Genet
112:1073-1085.

**Rostoks N, D Schmierer, S Mudie, T Drader, R Brueggeman, DG Caldwell,
R Waugh and A Kleinhofs. 2006**. Barley necrotic locus *nec1* encodes
the cyclic nucleotide-gated ion channel 4 homologous to the *Arabidopsis
HLM1*. Mol Gen Genomics 275:159-168.

**Szucs P, I Karsai, J von Zitzewitz, K Meszarus, LLD Cooper, YQ Gu, THH
Chen, PM Hayes and JS Skinner. 2006.** Positional relationships between
photoperiod response QTL and photoreceptor and vernalization genes in
barley. Theor Appl Genet 112:1277-1285.

**Taketa S, T Awayama, S Amano, Y Sakurai and M Ichii. 2006.**
High-resolution mapping of the *nud* locus controlling the naked
caryopsis in barley. Plant Breed 125:337-342.

**Tondelli A, E Francia, D Barbaschi, A Aprile, JS Skinner, EJ
Stockinger, AM Stanca and N Pecchioni. 2006.** Mapping regulatory genes
as candidates for cold and drought stress tolerance in barley. Theor
Appl Genet 112:445-454.

**Yan L, D Fu, C Li, A Blechl, G Tranquilli, M Bonafede, A Sanchez, M
Valarik, S Yasuda and J Dubcovsky. 2006.** The wheat and barley
vernalization gene *VRN3* is an orthologue of *FT*. PNAS
103:19581-19586.

**Yun SJ, L Gyenis, E Bossolini, PM Hayes, I Matus, KP Smith, BJ
Steffenson, R Tuberosa and GJ Muehlbauer. 2006.** Validation of
quantitative trait loci for multiple disease resistance in barley using
advanced backcross lines developed with a wild barley. Crop Sci
46:1179-1186.

# Integrating Molecular and Morphological/Physiological Marker Maps

**A. Kleinhofs**

**Dept. Crop and Soil Sciences and**

**School of Molecular Biosciences**

**Washington State University**

**Pullman, WA 99164-6420, USA**

**e-mail: andyk@wsu.edu**

There has been only limited progress in mapping morphological markers
during the past year. However, there is a major effort under way in
Europe to map the morphological mutant isolines developed by Jerry
Franckowiak (for details see
[[http://www.smallgraincereals.org/SGCNewsletterSummer2007.pdf]{.underline}](http://www.smallgraincereals.org/SGCNewsletterSummer2007.pdf)).
This effort, when completed, should provide a map of 1000 morphological
markers with accurate reference to molecular markers.

A recessive barley stripe rust resistance gene *rpsGZ* (from Grannenlose
Zweizeilige) was mapped to chromosome 4H bin9 (Yan and Chen, 2006). This
gene cosegregated with several RGAP markers identified in the
publication complete with primer information. It is also closely linked
to SSR markers EBmac0679 and EBmac0701.

Septoria speckled leaf blotch resistance genes *Rsp1*, *Rsp2*, and
*Rsp3* were mapped (Lee and Neate, 2007a). *Rsp2* cosegregated with
MWG938 placing it on chromosome 5(1H) bin2. *Rsp3* was closely linked to
*Rsp2* on chromosome 5S(1HS). *Rsp2* was flanked by RAPD markers
OPBA12314C and OPB17451R at 2.4 and 3.5 cM, respectively. I was not able
to locate these to a specific bin. *Rsp1* was mapped to chromosome 3H
short arm flanked by RAPD markers OPC2441R (3.0 cM) and UBC285158R (4.3
cM). In addition closely linked DArT markers were also identified.
Specific bin location was not possible. The RAPD markers were converted
into sequence tagged markers and primer sequences published (Lee and
Neate, 2007b).

Aluminum tolerance gene *Alp* was mapped to chromosome 4H bin7 closely
linked to ABG715 and cosegregating with several markers including HvMATE
(AV942930) which was proposed as a candidate gene for the *Alp* locus
(Wang *et al*., 2007)

Five barley *flowering locus T*-like (*FT*-like) genes were mapped
(Faure *et al*., 2007). *HvFT1* maps on chromosome 1(7H) between markers
AF022725 and Bmac31 closely linked to *VRN-H3* and ABC158 placing it in
bin4. *The VRN-H3* gene was previously believed to be located on
chromosome 5(1H), but more recently shown to be on chromosome 1(7H) (Yan
*et al*., 2006). *HvFT2* was mapped on chromosome 3H between markers
Bmac067 and MWG985 placing it in bin6. *HvHT3* was mapped to chromosome
7(5H) cosegregating with PSR162 placing it in bin11. *HvFT4* was mapped
to the short arm of chromosome 2H proximal to cMWG663 placing it in
bin6. *HvHT5* was mapped to chromosome 4H long arm cosegregating with
scsnp20989. It was not possible for me to determine the bin placement.

The naked caryopsis gene (*nud*) has been previously mapped to
chromosome 1(7H) bin 7. It has now been mapped at a high resolution
(Taketa *et al*., 2006). The closest SCAR marker SKT9, mapped 0.06 cM
from the *nud* locus based on 4,760 gametes from 6 mapping populations.

Barley lipoxygenase (Lox-1) thermostability factor was shown to
cosegregate with the structural gene *LoxA* (Hirota *et al*., 2006). The
*LoxA* and *LoxC* loci were previously mapped to chromosome 4H bin3 and
chromosome 7(5H) probably bin 10 (van Mechelen *et al*., 1999).

Barley homologs of large number of Arabidopsis low temperature
regulatory genes were mapped assigning either linkage map or chromosome
locations to 1 *ICE1*, 2 *ZAT12* and 17 *CBF* homologs (Skinner *et
al*., 2006). Eleven of the CBF genes with assigned linkage map positions
formed two tandem clusters on 5HL(7L). These were coincident with
reported Triticeae low temperature tolerance and *CO R* gene
accumulation QTL and suggest that one or more of the *CBF* genes may be
candidates for *Fr-H2* QTL.

Bin Assignments for Morphological Map Markers and closest molecular
marker

\* - indicates that the gene has been cloned

red - indicates that the gene is very accurately mapped with molecular
markers

yellow - indicates that it is fairly accurately mapped with molecular
markers

blue - indicates that the gene has been approximately mapped mainly
using Bulked

Segregant Analysis

Chr.1(7H)

BIN1 ABG704

\*[Rpg1]{.mark} RSB228 Brueggeman *et al*., PNAS 99:9328, '02

Run1

[Rdg2a]{.mark} MWG851A Bulgarelli *et al*., TAG 108:1401, '04

[Rrs2]{.mark} MWG555A Schweizer *et al*., TAG 90:920, '95

mlt

[brh1]{.mark} MWG2074B Li et al., 8^th^ IBGS 3:72, '00

BIN2 ABG320

Est5 iEst5 Kleinhofs *et al*., TAG 86:705, '93

[fch12]{.mark} BCD130 Schmierer *et al*., BGN 31:12, '01

\*[wax]{.mark} Wax Kleinhofs BGN 32:152, '02

[gsh3]{.mark} His3A Kleinhofs BGN 32:152, '02

BIN3 ABC151A

[fch5]{.mark} ABC167A Kleinhofs BGN 32:152, '02

[Rcs5]{.mark} KAJ185 Johnson & Kleinhofs, unpublished

yvs2

[cer-ze]{.mark} ABG380 Kleinhofs BGN 27:105, '96

BIN4 ABG380

wnd

\*HvFT1 ABC158 Faure *et al*., Genetics 176:599, \'07

VrnH3 ABC158 Yan *et al*., PNAS 103:19581, \'06

[Lga]{.mark} BE193581 Johnson & Kleinhofs, unpublished

abo7

BIN5 ksuA1A

ant1

[nar3]{.mark} MWG836 Kleinhofs BGN 32:152, '02

ert-m

ert-a

BIN6 ABC255

ert-d

fch8

fst3

cer-f

msg14

BIN7 ABG701

dsp1 cMWG704 Sameri & Komatsuda JARQ 41:195, \'07

msg10

[rsm1]{.mark} ABC455 Edwards & Steffenson, Phytopath. 86:184,'96

sex6

seg5

seg2

[pmr]{.mark} ABC308 Kleinhofs BGN 27:105, '96

[mo6b]{.mark} Hsp17 Soule *et al*., J Her. 91:483, '00

[nud]{.mark} sKT9 Taketa *et al*., Plant Breeding 125:337, \'06

[fch4]{.mark} MWG003 Kleinhofs BGN 27:105, '96

BIN8 \*[Amy2]{.mark} Amy2 Kleinhofs *et al*., TAG 86:705, '93

[lks2]{.mark} WG380B Costa *et al*., TAG 103:415, '01

ubs4

blx2

BIN9 RZ242

lbi3

[Rpt4]{.mark} Psr117D Williams *et al*., TAG 99:323, '99

xnt4

[lpa2]{.mark} ? Larson *et al*., TAG 97:141, '98

msg50

Rym2

seg4

BIN10 ABC310B

[Xnt1]{.mark} BF626025 Hansson *et al*., PNAS 96:1744, '99

[xnt-h]{.mark} BF626025 Hansson *et al*., PNAS 96:1744, '99

BIN11 ABC305

[Rph3]{.mark}

Tha2 Toojinda *et al*., TAG 101:580, '00

BIN12 ABG461A

Mlf

xnt9

seg1

msg23

BIN13 Tha

Rph19 Rlch4(Nc) Park & Karakousis Plt. Breed. 121:232. '02

Chr.2(2H)

BIN1 MWG844A

sbk

brh3 Bmac0134 Dahleen *et al*., J. Heredity 96:654, '05

BIN2 ABG703B

BIN3 MWG878A [gsh6]{.mark} Kleinhofs BGN 32:152, '02

gsh1

gsh8

BIN4 ABG318

Eam1

[Ppd-H1]{.mark} MWG858 Laurie *et al*., Heredity 72:619, '94

sld2

rtt

flo-c

sld4

BIN5 ABG358

fch15

brc1

com2

BIN6 Pox

msg9

abo2

Rph15 P13M40 Weerasena *et al*., TAG 108:712 '04

[rph16]{.mark} MWG874 Drescher *et al*., 8thIBGS II:95, '00

BIN7 Bgq60

[yst4]{.mark} CDO537 Kleinhofs BGN 32:152, '02

[Az94]{.mark} CDO537 Kleinhofs BGN 32:152, '02

[gai]{.mark} MWG2058 Börner *et al*., TAG 99:670, '99

msg33

[\*HvCslF]{.mark} (barley Cellulose synthase-like) Burton *et al*.,
Science 311:1940 '06

[\*Bmy2]{.mark}

msg3

fch1

BIN8 ABC468

[Eam6]{.mark} ABC167b Tohno-oka *et al*., 8thIBGS III:239, '00

gsh5

msg2

[eog]{.mark} ABC451 Kleinhofs BGN 27:105, '96

abr

cer-n

BIN9 ABC451

Gth

hcm1

wst4

\*[vrs1]{.mark} MWG699 Komatsuda *et al*., Genome 42:248, '00

BIN10 MWG865

cer-g

Lks1

mtt4

Pre2

msg27

BIN11 MWG503

[Rha2]{.mark} AWBMA21 Kretschmer *et al*., TAG 94:1060, '97

Ant2 MWG087 Freialdenhoven *et al*., Plt. Cell 6:983, '94

\*[Rar1]{.mark} AW983293B Freialdenhoven *et al*., Plt. Cell 6:983, '94

fol-a

[gal]{.mark} MWG581A Börner *et al*., TAG 99:670, '99

fch14

Pau

BIN12 ksuD22

Pvc

BIN13 ABC252

[lig]{.mark} BCD266 Pratchett & Laurie Hereditas 120:35, '94

[nar4]{.mark} Gln2 Kleinhofs BGN 27:105, '96

[Zeo1]{.mark} cnx1 Costa *et al*., TAG 103:415, '01

[lpa1]{.mark} ABC157 Larson *et al*., TAG 97:141, '98

BIN14 ABC165

BIN15 MWG844B

[gpa]{.mark} CDO036 Kleinhofs BGN 27:105, '96

[wst7]{.mark} MWG949A Costa *et al*., TAG 103:415, '01

[MlLa]{.mark} Ris16 Giese *et al*., TAG 85:897, '93

trp

Chr. 3(3H)

BIN1 [Rph5]{.mark} ABG070 Mammadov *et al*., TAG 111:1651, '05

[Rph6]{.mark} BCD907 Zhong et al., Phytopath. 93:604, '03

[Rph7]{.mark} MWG848 Brunner *et al*., TAG 101:783, '00

BIN2 JS195F BI958652; BF631357; BG369659

ant17

sld5

[mo7a]{.mark} ABC171A Soule et al., J. Hered. 91:483, '00

brh8

BIN3 ABG321

xnt6

BIN4 MWG798B

[btr1]{.mark} Senthil & Komatsuda Euphytica 145:215, '05

[btr2]{.mark} Senthil & Komatsuda Euphytica 145:215, '05

lzd

[alm]{.mark} ABG471 Kleinhofs BGNL 27:105, '96

BIN5 BCD1532

abo9

sca

yst2

dsp10

BIN6 ABG396

[Rrs1]{.mark} Graner *et al*., TAG 93: 421 ´96

[Rh/Pt]{.mark} ABG396 Smilde *et al*., 8th IBGS 2:178, '00

[Rrs.B87]{.mark} BCD828 Williams *et al*., Plant Breed. 120:301, '01

AtpbB

abo6

xnt3

HvHT2 Bmac067 Faure *et al*., Genetics 176:599, \'07

msg5

ari-a

yst1

zeb1

ert-c

ert-ii

cer-zd

[Ryd2]{.mark} WG889B Collins *et al*., TAG 92:858, '96

\*[uzu]{.mark} AB088206 Saisho *et al*., Breeding Sci. 54:409, '04

BIN7 MWG571B

cer-r

BIN8 ABG377

wst6

cer-zn

sld1

BIN9 ABG453

wst1

BIN10 CDO345

vrs4

Int1

gsh2

BIN11 CDO113B

als

[sdw1]{.mark} PSR170 Laurie *et al*., Plant Breed. 111:198, '93

BIN12 His4B

sdw2

BIN13 ABG004

[Pub]{.mark} ABG389 Kleinhofs *et al*., TAG 86:705, '93

BIN14 ABC161

cur2

BIN15 ABC174

Rph10

fch2

BIN16 ABC166

eam10

Est1/2/3

\*[rym4]{.mark} *eIF4E* Stein *et al*.,Plt. J. 42:912, '05

\*[rym5]{.mark} *eIF4E* and Kanyuka *et al*., Mol. Plant Path. 6:449,
'05

Est4

ant28

Chr.4(4H)

BIN1 MWG634

BIN2 JS103.3

fch9

sln

BIN3 Ole1

[Dwf2]{.mark} Ivandic *et al*., TAG 98:728, '99

\*LoxA MWG011b van Mechelen *et al*., Plt. Mol. Biol. 39:1283, \'99

LoxB van Mechelen *et al*., Plt. Mol. Biol. 39:1283, \'99

Lox-1 thermo Hirota *et al*., Plant breeding 125:231, \'06

Ynd

int-c MWG2033 Komatsuda, TAG 105:85, '02

Zeo3

glo-a

rym1 MWG2134 Okada et al., Breeding Sci. 54:319, '04

BIN4 BCD402B

\*[Kap]{.mark} X83518 Müller *et al*., Nature 374:727, '95

lbi2

zeb2

lgn3

BIN5 BCD808B

lgn4

lks5

eam9

msg24

BIN6 ABG484

glf1

[rym11]{.mark} MWG2134 Bauer *et al*., TAG 95:1263, '97

[Mlg]{.mark} MWG032 Kurth *et al*., TAG 102:53, '01

cer-zg

brh2

BIN7 bBE54A

glf3

Alp HvMATE Wang *et al*., TAG 115:265 \'07

frp

min1

blx4

sid

blx3

BIN8 BCD453B

blx1

BIN9 ABG319A

ert1

rpsGZ EBmac0679 Yan & Chen, TAG 113:529, \'06

BIN10 KFP221

\*[mlo]{.mark} P93766 Bueschges *et al*., Cell 88:695, '97

BIN11 ABG397

BIN12 ABG319C

[Hsh]{.mark} HVM067 Costa *et al*., TAG 103:415, '01

Hln

\*[sgh1]{.mark}(ZCCT-H; HvSnf2) Zitzewitz *et al*., PMB 59:449, '05

yhd1

BIN13 \*[Bmy1]{.mark} pcbC51 Kleinhofs *et al*., TAG 86:705, '93

[rym8]{.mark} MWG2307 Bauer *et al*., TAG 95:1263, '97

[rym9]{.mark} MWG517 Bauer *et al*., TAG 95:1263, '97

Wsp3

Chr. 5(1H)

BIN1 Tel5P

Rph4

Mlra

Cer-yy

[Sex76]{.mark} Hor2 Netsvetaev BGN 27:51, '97

\*[Hor5]{.mark} Hor5 Kleinhofs *et al*., TAG 86:705, '93

BIN2 MWG938

Rsp2 MWG938 Lee & Neate, Phytopath. 97:155, \'07

\*[Hor2]{.mark} Hor2 Kleinhofs *et al*., TAG 86:705, '93

[Rrs14]{.mark} Hor2 Garvin *et al*., Plant Breed. 119:193-196, '00

\*[Mla6]{.mark} AJ302292 Halterman *et al*., Plt J. 25:335, '01

BIN3 MWG837

\*[Hor1]{.mark} Hor1 Kleinhofs *et al*., TAG 86:705, '93

Rps4

Mlk

BIN4 ABA004

Lys4

BIN5 BCD098

Mlnn;

msg31;

sls;

msg4;

fch3;

BIN6 Ica1

amo1

BIN7 JS074

clh

vrs3

Ror1 ABG452 Collins *et al*., Plt. Phys. 125:1236, '01

BIN8 Pcr2

fst2

cer-zi

cer-e

ert-b

MlGa

msg1

xnt7

BIN9 Glb1

\*[nec1]{.mark} BF630384 Rostoks *et al*., MGG 275:159, \'06

BIN10 DAK123B

abo1

Glb1

BIN11 PSR330

\*HvFT3 PSR162 Faure *et al*., Genetics 176:599, \'07

PpdH2 PSR162 Laurie *et al*., Genome 38:575, \'95

wst5

cud2

BIN12 MWG706A

rlv

lel1

BIN13 BCD1930

[Blp]{.mark} ABC261 Costa *et al*., TAG 103:415, '01

BIN14 ABC261

fch7

trd

eam8

Chr. 6(6H)

BIN1 ABG062

\*[Nar1]{.mark} X57845 Kleinhofs *et al*., TAG 86:705, '93

abo15

BIN2 ABG378B

[nar8]{.mark} ABG378B Kleinhofs BGN 27:105, '96

nec3

Rrs13

BIN3 MWG652A

BIN4 DD1.1C

msg36

BIN5 ABG387B

nec2

ant21

msg6

eam7

BIN6 Ldh1

[rob]{.mark} HVM031 Costa *et al*., TAG 103:415, '01

sex1

gsh4

ant13

[cul2]{.mark} Crg4(KFP128) Babb & Muehlbauer BGN 31:28, '01

fch11

mtt5

abo14

BIN7 ABG474

BIN8 ABC170B

BIN9 \*[Nar7]{.mark} X60173 Warner *et al*., Genome 38:743, '95

\*[Amy1]{.mark} JR115 Kleinhofs *et al*., TAG 86:705, '93

\*[Nir]{.mark} pCIB808 Kleinhofs *et al*., TAG 86:705, '93

mul2

cur3

BIN10 MWG934

lax-b

raw5

cur1

BIN11 Tef1

BIN12 xnt5

Aat2

BIN13 [Rph11]{.mark} Acp3 Feuerstein *et al*., Plant breed. 104:318, '90

lax-c

BIN14 DAK213C

dsp9

Chr. 7(5H)

BIN1 DAK133

abo12

msg16

ddt

BIN2 MWG920.1A

dex1

msg19

nld

fch6

glo-b

BIN3 [cud1]{.mark} ABG705A

lys3

fst1

blf1

vrs2

BIN4 ABG395

cer-zj

cer-zp

msg18

wst2

[Rph2]{.mark} ITS1 Borovkova *et al*., Genome 40:236, '97

[lax-a]{.mark} PSR118 Laurie *et al*., TAG 93:81, '96

com1

ari-e

ert-g

ert-n

BIN5 Ltp1

[rym3]{.mark} MWG028 Saeki *et al*., TAG 99:727, '99

BIN6 WG530

BIN7 ABC324

BIN8 ABC302A

BIN9 BCD926

[srh]{.mark} ksuA1B Kleinhofs *et al*., TAG 86:705, '93

cer-i

mtt2

lys1

cer-t

dsk

var1

cer-w

Eam5

BIN10 ABG473

raw1

msg7

BIN11 MWG514B

[Rph9/12]{.mark}ABG712 Borokova *et al*., Phytopath. 88:76, '98

\*[Sgh2]{.mark} (HvBM5A) Zitzewitz *et al*., PMB 59:449, '05

\*[Ror2]{.mark} AY246906 Collins *et al*., Nature 425:973, '03

lbi1

Rha4

raw2

BIN12 WG908

none

BIN13 ABG496

[rpg4]{.mark} ARD5303 Druka *et al*., unpublished

[RpgQ]{.mark} ARD5304 Druka *et al*., unpublished

BIN14 ABG390

var3

BIN15 ABG463

**References:**

**Babb, S.L. and G.J. Muehlbauer. 2001.** Map location of the Barley
Tillering Mutant *uniculm2* (*cul2*) on Chromosome 6H. BGN31:28.

**Bauer, E., J. Weyen, A. Schiemann, A. Graner, and F. Ordon. 1997.**
Molecular mapping of novel resistance genes against Barley Mild Mosaic
Virus (BaMMV). Theor. Appl. Genet. 95:1263-1269.

**Borovkova, I.G., Y. Jin, B.J. Steffenson, A. Kilian, T.K. Blake, and
A. Kleinhofs. 1997.** Identification and mapping of a leaf rust
resistance gene in barley line Q21861. Genome 40:236-241.

**Borokova, I.G., Y. Jin, and B.J. Steffenson. 1998.** Chromosomal
Location and Genetic Relationship of Leaf Rust Resistance Genes *Rph9*
and *Rph12* in Barley. Phytopathology 88:76-80.

**Börner, A., V. Korzun, S. Malyshev, V. Ivandic, and A. Graner. 1999.**
Molecular mapping of two dwarfing genes differing in their GA response
on chromosome 2H of barley. Theor. Appl. Genet. 99:670-675.

**Brueggeman, R., N. Rostoks, D. Kudrna, A. Kilian, F. Han, J. Chen, A.
Druka, B. Steffenson, and A. Kleinhofs. 2002.** The barley stem-rust
resistance gene *Rpg1* is a novel disease-resistance gene with homology
to receptor kinases. Proc. Natl. Acad. Sci. USA 99:9328-9333.

**Brunner, S., B. Keller, and C. Feuillet. 2000.** Molecular mapping of
the Rph7.g leaf rust resistance gene in barley (*Hordeum vulgare* L.).
Theor. Appl. Genet. 101:763-788.

**Büschges, R., K. Hollricher, R. Panstruga, G. Simons, M. Wolter, A.
Frijters, R. van Daelen, T. van der Lee, P. Diergaarde, J. Groenendijk,
S. Töpsch, P. Vos, F. Salamini, and P. Schulze-Lefert. 1997.** The
barley *mlo* gene: A novel control element of plant pathogen resistance.
Cell 88:695-705.

**Bulgarelli, D., N.C. Collins, G. Tacconi, E. Dellaglio, R. Brueggeman,
A. Kleinhofs, A.M. Stanca, and G. Vale. 2004.** High resolution genetic
mapping of the leaf stripe resistance gene Rdg2a in barley. Theor. Appl.
Genet. 108:1401-1408.

**Burton, R.A., S.M. Wilson, M. Hrmova, A.J. Harvey, N.J. Shirley, A.
Medhurst, B.A. Stone, E.J. Newbigin, A. Bacic, and G.B. Fincher. 2006.**
Cellulose synthase-like CslF genes mediate the synthesis of cell wall
(1,3; 1,4)-b-D-glucans. Science 311:1940-1943.

**Collins, N.C., N.G. Paltridge, C.M. Ford, and R.H. Symons.1996.** The
*Yd2* gene for barley yellow dwarf virus resistance maps close to the
centromere on the long arm of barley chromosome 3. Theor. Appl. Genet.
92:858-864.

**Collins, N.C., T. Lahaye, C. Peterhänsel, A. Freialdenhoven, M.
Corbitt, and P. Schulze-Lefert. 2001.** Sequence haplotypes revealed by
sequence-tagged site fine mapping of the *Ror1* gene in the centromeric
region of barley chromosome 1H. Plant Physiology 125:1236-1247.

**Collins, N.C., H. Thordal-Christensen, V. Lipka, S. Bau, E. Kombrink,
J-L. Qiu, R. Hückelhoven, N. Stein, A. Freialdenhoven, S.C. Somerville,
and P. Schulze-Lefert. 2003.** Snare-protein-mediated disease resistance
at the plant cell wall. Nature 425:973-976.

**Costa, J.M., A. Corey, M. Hayes, C. Jobet, A. Kleinhofs, A.
Kopisch-Obusch, S.F. Kramer, D. Kudrna, M. Li, O. Piera-Lizaragu, K.
Sato, P. Szues, T. Toojinda, M.I. Vales, and R.I. Wolfe. 2001.**
Molecular mapping of the Oregon Wolfe Barleys: a phenotypically
polymorphic doubled-haploid population. Theor. Appl. Genet. 103:415-424.

**Drescher, A., V. Ivandic, U. Walther, and A. Graner. 2000.**
High-resolution mapping of the Rph16 locus in barley. p. 95-97. *In*: S.
Logue (ed.) Barley Genetics VIII. Volume II. Proc. Eigth Int. Barley
Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide
University, Glen Osmond, South Australia.

**Dahleen, L.S., L.J. Vander Wal, and J.D. Franckowiak. 2005.**
Characterization and molecular mapping of genes determining semidwarfism
in barley. J. Heredity 96:654-662.

**Edwards, M.C. and B.J. Steffenson. 1996.** Genetics and mapping of
barley stripe mosaic virus resistance in barley. Phytopath. 86;184-187.

**Faure S, J. Higgins, A. Turner, and D.A. Laurie. 2007.** The
*FLOWERING LOCUS T*-like gene family in barley (*Hordeum vulgare*).
Genetics 176:599-609.

**Feuerstein, U., A.H.D. Brown, and J.J. Burdon. 1990.** Linkage of rust
resistance genes from wild barley (*Hordeum spontaneum*) with isoenzyme
markers. Plant. Breed. 104:318-324.

**Freialdenhoven, A., B. Scherag, K. Hollrichter, D.B. Collinge, H.
Thordal-Christensen, and P. Schulze-Lefert. 1994.** *Nar-1* and *Nar-2*,
two loci required for *Mla12*-specified race-specific resistance to
powdery mildew in barley. Plant Cell 6:983-994.

**Garvin, D.F., A.H.D. Brown, H. Raman, and B.J. Read. 2000.** Genetic
mapping of the barley *Rrs14* scald resistance gene with RLFP, isozyme
and seed storage protein marker. Plant Breeding 119:193-196.

**Giese, H., A.G. Holm-Jensen, H.P. Jensen, and J. Jensen. 1993.**
Localisation of the Laevigatum powdery mildew resistance gene to barley
chromosome 2 by the use of RLFP markers. Theor. Appl. Genet. 85:897-900.

**Graner, A. and A. Tekauz. 1996.** RFLP mapping in barley of a dominant
gene conferring to scald (*Rynchosporium secalis*). Theor. Appl. Genet.
93:421-425.

**Haltermann, D., F. Zhou, F. Wei, R.P. Wise, and P. Schulze-Lefert.
2001.** The MLA6 coiled coil, NBS-LRR protein confers
*AvrMla6*-dependent resistance specificity to *Blumeria graminis* f. sp.
*hordei* in barley and wheat. Plt. J. 25:335-348.

**Hansson, A., C.G. Kannangara, D. von Wettstein, and M. Hansson.
1999.** Molecular basis for semidomiance of missense mutations in the
XANTHA-H (42-kDa) subunit of magnesium chelatase. Proc. Natl. Acad. Sci.
USA 96:1744-1749.

**Hirota, N., T. Kaneko, K. Ito, and K. Takeda. 2006.** Mapping a factor
controlling the thermostability of seed lipoxygenase-1 in barley. Plant
Breeding 125:231-235.

**Ivandic, V., S. Malyshev, V. Korzun, A. Graner, and A. Börner. 1999.**
Comparative mapping of a gibberellic acid-insensitive dwarfing gene
(*Dwf2*) on chromosome 4HS in barley. Theor. Appl. Genet. 98:728-731.

**Kanyuka, K., A. Druka, D..G. Caldwell, A. Tymon, N. McCallum, R.
Waugh, and M. J. Adams. 2005.** Evidence that the recessive bymovirus
resistance locus *rym4* in barley corresponds to the eukaryotic
translation initiation factor 4E gene. Molecular Plant pathology
6:449-458.

**Kleinhofs, A., A. Kilian, M.A. Saghai Marrof, R.M. Biyashev, P. Hayes,
F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev,
L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorells, M.
Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen, and B.J. Steffenson.
1993.** A molecular, isozyme and morphological map of the barley
(*Hordeum vulgare*) genome. Theor. Appl. Genet. 86:705-712.

**Kleinhofs, A. 1996.** Integrating Barley RFLP and Classical Marker
Maps. Coordinator's report. BGN27:105-112.

**Kleinhofs, A. 2002.** Integrating Molecular and
Morphological/Physiological Marker Maps. Coordinator's Report.
BGN32:152-159.

**Komatsuda, T., W. Li, F. Takaiwa, and S. Oka. 1999.** High resolution
map around the *vrs1* locus controlling two- and six-rowed spike in
barley. (*Hordeum vulgare*). Genome 42:248-253.

**Komatsuda, T., and Y. Mano. 2002.** Molecular mapping of the
intermedium spike-c (*int-c*) and non-brittle rachis 1 (*btr1*) loci in
barley (*Hordeum vulgare* L.). Theor. Appl Genet. 105:85-90.

**Kretschmer, J.M., K.J. Chalmers, S. Manning, A. Karakousis, A.R. Barr,
A.K.M.R. Islam, S.J. Logue, Y.W. Choe, S.J. Barker, R.C.M. Lance, and P.
Langridge. 1997.** RFLP mapping of the *Ha2* cereal cyst nematode
resistance in barley.Theor. Appl. Genet. 94:1060-1064.

**Kurth, J., R. Kolsch, V. Simons, and P. Schulze-Lefert. 2001.** A
high-resolution genetic map and a diagnostic RFLP marker for the *Mlg*
resistance locus to powdery mildew in barley. Theor. Appl. Genet.
102:53-60.

**Larson, S.R., K.A. Young, A. Cook, T.K. Blake, and V. Raboy. 1998.**
Linkage mapping of two mutations that reduce phytic acid content of
barley grain. Theor. Appl. Genet. 97:141-146.

**Laurie, D.A., N. Pratchett, C. Romero, E. Simpson, and J.W. Snape.
1993.** Assignment of the *denso* dwarfing gene to the long arm of
chromosome 3 (3H) of barley by use of RFLP markers. Plant. Breed.
111:198-203.

**Laurie, D.A., N. Pratchett, J.H. Bezan, and J.W. Snape. 1994.**
Genetic analysis of a photoperiod response gene on the short arm of
chromosome 2 (2H) of Hordeum vulgare (barley). Heredity 72:619-627.

**Laurie, D.A., N. Pratchett, J.H. Bezan, and J.W. Snape. 1995.** RFLP
mapping of five major genes and eight quantitative trait loci
controlling flowering time in a winter x spring barley (*Hordeum
vulgare* L.) cross. Genome 38:575-585.

**Laurie, D.A., N. Pratchett, R.A. Allen, and S.S. Hantke. 1996.** RFLP
mapping of the barley homeotic mutant *lax-a*. Theor. Appl. Genet.
93:81-85.

**Lee S.H. and S.M. Neate. 2007a.** Molecular mapping of *Rsp1*, *Rsp2*,
and *Rsp3* genes conferring resistance to Septoria speckled leaf blotch
in barley. Phytopathology 97:155-161.

**Lee S.H. and S.M. Neate. 2007b.** Sequence tagged site markers to
*Rsp1*, *Rsp2*, and *Rsp3* genes for resistance to Septoria speckled
leaf blotch in barley. Phytopathology 97:161-169.

**Li. M., D. Kudrna, and A. Kleinhofs. 2000.** Fine mapping of a
Semi-dwarf gene Brachytic1 in barley. p. 72-74. *In*: S. Logue (ed.)
Barley Genetics VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp.
Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen
Osmond, South Australia.

**Mammadov, J.A., B. J. Steffenson, and M.A. Saghai Maroof. 2005.** High
resolution mapping of the barley leaf rust resistance gene Rph5 using
barley expressed sequence tags (ESTs) and synteny with rice. Theor.
Appl. Genet. 111:1651-1660.

**Mechelen J.R. van, R.C. Schuurink, M. Smits, A. Graner, A.C. Douma,
N.J.A. Sedee, N.F. Schmitt, and B.E Valk. 1999.** Molecular
characterization of two lipoxygenases from barley. Plant Molecular
Biology 39:1283-1298.

**Müller, K.J., N. Romano, O. Gerstner, F. Gracia-Maroto, C. Pozzi, F.
Salamini, and W. Rhode. 1995.** The barley *Hooded* mutation caused by a
duplication in a homeobox gene intron. Nature 374:727-730.

**Netsvetaev, V.P. 1997.** High lysine mutant of winter barley - L76.
BGN27:51-54.

**Okada, Y., R. Kanatani, S. Arai, and I. Kazutoshi. 2004.** Interaction
between barley mosaic disease-resistance genes *rym1* and *rym5*, in the
response to BaYMV strains. Breeding Science 54 (4):319-324.

**Park, R.F. and A. Karakousis. 2002.** Characterization and mapping
*Rph19* conferring resistance to *Puccinia hordei* in the cultivar
'Rea1' and several Australian barley. Plant Breeding 121:232-236.

**Pratchett, N. and D.A. Laurie. 1994.** Genetic map location of the
barley developmental mutant *liguleless* in relation to RFLP markers.
Hereditas 120:35-39.

**Rostoks, N., D. Schmierer, S. Mudie, T. Drader, R. Brueggeman, D.
Caldwell, R. Waugh, and A. Kleinhofs. 2006.** Barley necrotic locus
*nec1* encodes the cyclic nucleotide-gated ion channel 4 homologous to
the *Arabidopsis Hlm1.* Mol.Gen. Genomics 275:159-168.

**Saeki, K., C. Miyazaki, N. Hirota, A. Saito, K. Ito, and T. Konishi.
1999.** RFLP mapping of BaYMV resistance gene *rym3* in barley (*Hordeum
vulgare*). Theor. Appl. Genet. 99:727-732.

**Saisho, D., K.-I. Tanno, M. Chono, I. Honda, H. Kitano, and K. Takeda.
2004.** Spontaneous Brassinolide-insensitive barley mutants 'uzu'
adapted to East Asia. Breeding Science 54(4): 409-416.

**Sameri, M. and T. Komatsuda. 2007.** Location of quantitative trait
loci for yield components in a cross oriental x occidental barley
cultivar (*Hordeum vulgare* L.). Japan Agricultural Research Quaterly
41(3):195-199.

**Schmierer, D., A. Druka, D. Kudrna, and A. Kleinhofs. 2001.** Fine
Mapping of the *fch12* chlorina seedling mutant. BGN31:12-13.

**Schweizer, G.F., M. Baumer, G. Daniel, H. Rugel, and M.S. Röder.
1995.** RFLP markers linked to scald (*Rhynchosporium secalis*)
resistance gene *Rh2* in barley. Theor. Appl. Genet. 90:920-922.

**Skinner J.S., P. Szucs, J. von Zitzewitz, L. Marquez-Cedillo, T.
Filichkin, E.J. Stockinger, M.F. Thomashow, T.H.H. Chen, and P.M. Hayes.
2006.** Mapping of barley homologs to genes that regulate low
temperature tolerance in Arabidopsis. Theor. Appl. Genet. 112:832-842.

**Smilde, W.D., A. Tekauz, and A. Graner. 2000.** Development of a high
resolution map for the *Rh* and *Pt* resistance on barley Chromosome 3H.
p. 178-180. *In*: S. Logue (ed.) Barley Genetics VIII. Volume II. Proc.
Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite
Campus, Adelaide University, Glen Osmond, South Australia.

**Soule, J.D., D.A. Kudrna, and A. Kleinhofs. 2000.** Isolation,
mapping, and characterization of two barley multiovary mutants. J.
Heredity 91:483-487.

**Senthil, N. and T. Komatsuda. 2005.** Inter-subspecific maps of
non-brittle rachis gene btr1/btr2 using occidental, oriental and wild
barley lines. Euphytica 145:215-220.

**Stein, N., D. Perovic, J. Kumlehn, B. Pellio, S. Stracke, S. Streng,
F. Ordon, and A. Graner. 2005.** The eukaryotic translation initian
factor 4E confers multiallelic recessive Bymovirus resistance in
*Hordeum vulgare* (L.). The Plant Journal 42:912-922.

**Taketa, S., T. Awayama, S. Amano, Y. Sakurai, and M. Ichii. 2006.**
High-resolution mapping of the *nud* locus controlling the naked
caryopsis in barley. Plant Breeding 125:337-342.

**Tohno-oka, T., M. Ishii, R. Kanatani, H. Takahashi, and K. Takeda.
2000.** Genetic Analysis of photoperiotic response of barley in
different daylength conditions. p.239-241. *In*: S. Logue (ed.) Barley
Genetics VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp.
Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen
Osmond, South Australia.

**Toojinda, T., L.H. Broers, X.M. Chen, P.M. Hayes, A. Kleinhofs, J.
Korte, D. Kudrna, H. Leung, R.F. Line, W. Powell, L. Ramsey, H. Vivar,
and R. Waugh. 2000.** Mapping quantitative and qualitative disease
resistance genes in a doubled haploid population of barley (*Hordeum
vulgare*). Theor. Appl. Genet. 101:580-589.

**Wang, J., H. Raman, M. Zhou, P.R. Ryan, E. Delhaize, D.M. Hebb, N.
Coombes, and N. Mendham. 2007.** High-resolution mapping of the Alp
locus and identification of a candidate gene *HvMATE* controlling
aluminum tolerance in barley (*Hordeum vulgare* L.). Theor. Appl. Genet.
115:265-276.

**Warner, R.L., D.A. Kudrna, and A. Kleinhofs. 1995.** Association of
the NAD(P)H-bispecific nitrate reductase structural gene with the *Nar7*
locus in barley. Genome 38:743-746.

**Weerasena, J.S., B.J. Steffenson, and A.B. Falk. 2004.** Conversion of
an amplified fragment length polymorphism marker into a c-dominant
marker in mapping *Rph15* gene conferring resistance to barley leaf
rust, *Puccinia hordei* Otth. Theor. Appl. Genet. 108:712-719.

**Williams, K.J., A. Lichon, P. Gianquitto, J.M. Kretschmer, A.
Karakousis, S. Manning, P. Langridge, and H. Wallwork. 1999.**
Identification and mapping of a gene conferring resistance to the spot
form of net blotch (*Pyrenophora teres* f. *maculata*) in barley. Theor.
Appl. Genet. 99: 323-327.

**Williams, K., P. Bogacki, L. Scott, A. Karakousis, and H. Wallwork.
2001.** Mapping of a gene for leaf scald resistance in barley line
'B87/14' and validation of microsatellite and RFLP markers for
marker-assisted selection. Plant Breed. 120:301-304.

**Yan G.P., and X.M. Chen. 2006.** Molecular mapping of a recessive gene
for resistance to stripe rust in barley. Theor. Appl. Genet.
113:529-537.

**Yan L., D. Fu, C. Li, A. Blechl, G. Tranquilli, M. Bonafede, A.
Sanchez, M. Valarik, S. Yasuda, and J. Dubcovsky. 2006.** The wheat and
barley vernalization gene *VRN3* is an orthologue of *FT*. Proc. Natl.
Acad. Sci. USA 103:19581-19586.

**Zhong, S.B., R.J. Effertz, Y. Jin, J.D. Franckowiak, and B.J.
Steffenson. 2003.** Molecular mapping of the leaf rust resistance gene
*Rph6* in barley and its linkage relationships with *Rph5* and *Rph7*.
Phytopathology 93 (5):604-609.

**Zitzewitz, J. von, P. Scucs, J. Dobcov, L. Yan, E. Francia, N.
Pecchioni, A. Casas, T.H.H. Chen, P.M. Hayes, and J.S. Skinner. 2005.**
Molecular and structural characterization of barley vernalization genes.
Plant Molecular Biology 59:449-467.

**Coordinator's report: Barley Genetic Stock Collection**

# A. Hang and K. Satterfield

**USDA-ARS, National Small Grains Germplasm Research Facility,**

**Aberdeen, Idaho 83210, USA**

**e-mail: [anhang@uidaho.edu]{.underline}**

In 2006, 655 barley genetic stocks were planted in the field and in the
greenhouse for evaluation and for seed increase.

Two mapping populations, including SSD F6 seed OSU 11/Harrington and SSD
F6 seed OSU 15/Harrington, derived from single seed descent (SSD) of
crosses between *Hordeum vulgare subps. Spontaneum* and cultivar
"Harrington" obtained from Dr. Pat Hayes, Oregon State University (OSU),
were planted in the field for seed increase.

Four necrotic or lesion mimic mutants obtained from Dr. Anders Falk,
Biological Research Center, Sweden, were also grown in the greenhouse
for observation and for seed increase.

Three hundred forty-five samples of barley genetic stocks were shipped
to researchers in 2006.

## Coordinator's report: Trisomic and aneuploid stocks

**An Hang**

**USDA-ARS, National Small Grains Germplasm Research Facility,**

**Aberdeen, Idaho 83210, USA**

**e-mail: [anhang@uidaho.edu]{.underline}**

There is no new information about trisomic and aneuploid stocks. Lists
of these stocks are available in BGN 25:104. Seed request for these
stocks should be sent to the coordinator.

**Coordinator's report: Translocations and balanced tertiary trisomics**

**Andreas Houben**

**Institute of Plant Genetics and Crop Plant Research**

**DE-06466 Gatersleben, Germany**

**email: houben@ipk-gatersleben.de**

Restructured barley chromosomes have been used by Nasuda et al. (2005)
to elucidate the function of centromere-localized DNA sequences. The
satellite sequences (AGGGAG)(n) and Ty3/gypsy-like retrotransposons are
known to localize at the barley centromeres. Using a gametocidal system,
which induces chromosomal mutations in barley chromosomes added to
common wheat, the authors obtained an isochromosome for the short arm of
barley chromosome 7H (7HS) that lacked the barley-specific satellite
sequence (AGGGAG)(n). Two telocentric derivatives of the isochromosome
arose in the progeny: 7HS\* with and 7HS\*\* without the pericentromeric
C-band. FISH analysis demonstrated that both truncated telosomes lacked
not only the barley-specific centromeric repeats but also any of the
known wheat centromeric tandem repeats. Although they lacked these
centromeric repeats, both truncated telochromosomes showed normal
mitotic and meiotic transmission. Indirect immunostaining revealed that
centromere-specific proteins localized at the centromeric region of
7HS\*. The authors conclude that the barley centromeric repeats are
neither sufficient nor obligatory to assemble kinetochores, and
discussed the possible formation of a novel centromere in a barley
chromosome.

The collection is being maintained in cold storage. To the best
knowledge of the coordinator, there are no new publications dealing with
balanced tertiary trisomics in barley. Limited seed samples are
available any time, and requests can be made to the coordinator.

**Reference:**

**Nasuda, S., Hudakova, S., Schubert, I., Houben, A., and Endo, T.R.
2005.** Stable barley chromosomes without centromeric repeats. Proc Natl
Acad Sci U S A 102: 9842-9847.

**Coordinator's report: Autotetraploids**

**Wolfgang Friedt**

**Institute of Crop Science and Plant Breeding I.**

**Justus-Liebig-University, Heinrich-Buff-Ring 26-32**

**DE-35392 Giessen, Germany**

**e-mail: <wolfgang.friedt@agrar.uni-giessen.de>**

**Fax: +49(0)641-9937429**

The collection of barley autotetraploids (exclusively spring types)
described in former issues of BGN is maintained at the Giessen Field
Experiment Station of our institute. The set of stocks, i.e.
autotetraploids (4n) and corresponding diploid (2n) progenitors (if
available) have last been grown in the field for seed multiplication in
summer 2000. Limited seed samples of the stocks are available for
distribution.

**Coordinator's report: *Eceriferum* Genes**

**Udda Lundqvist**

### Nordic Gene Bank

**P.O. Box 41, SE-230 53 Alnarp, Sweden**

**e-mail: udda@nordgen.org**

Dahleen and Franckowiak (2006) could localize the *eceriferum*-zt locus
on chromosome 2HS based on their molecar linkage studies and found
linkage in bin 2H-01 d 16.8 distal from the SSR molecular marker Bmac
0134. Surface wax coating on the spike appears slightly reduced with
*eceriferum*-zt. The wax code for this mutant gene is + ++ ++ .

No further research work on gene localization has been reported on these
collections of *Eceriferum* and *Glossy* genes. All descriptions in
Barley Genetics Newsletter (BGN) Volume 26 are valid and still
up-to-date. All Swedish *Eceriferum* alleles can be seen in the SESTO
database of the Nordic Gene Bank. Descriptions, images and graphic
chromosome map displays of the *Eceriferum* and *Glossy* genes are
available in the AceDB database for Barley Genes and Barley Genetic
Stocks, and they get currently updated. Its address is found by:
[www.untamo.net/bgs](http://www.untamo.net/bgs)

As my possibilities in searching literature are very limited, I
apologize if I am missing any important papers. Please send me notes of
publications and reports to include in next year's reports.

Every research of interest in the field of *Eceriferum* genes, 'Glossy
sheath' and 'Glossy leaf' genes can be reported to the coordinator as
well. Seed requests regarding the Swedish mutants can be forwarded to
the coordinator [udda@nordgen.org]{.underline} or to the Nordic Gene
Bank, [www.nordgen.org/ngb](mailto:nordgen@ngb.se), all others to the
Small Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210,
USA, <nsgchb@ars-grin.gov> or to the coordinator at any time.

**Reference:**

**Dahleen, L.S. and J.D. Franckowiak. 2006.** SSR linkages to eight
additional morphological marker traits. Barley Genetic Newsletter
36:12+16.

**Coordinator's report: Nuclear genes affecting the chloroplast**

**Mats Hansson**

**Department of Biochemistry,**

**Lund University, Box 124,**

**SE-22100 Lund, Sweden**

**E-mail: [mats.hansson@biochemistry.lu.se]{.underline}**

Barley nuclear mutants deficient in chlorophyll biosynthesis and
chloroplast development are named *albina*, *xantha*, *viridis*,
*chlorina*, *tigrina* and *striata* depending on their colour and colour
pattern. In the *albina* mutants the leaves are completely white due to
lack of both chlorophyll and carotene pigments. The *xantha* mutants are
yellow and produce carotene, but no chlorophyll. The *chlorina* and
*viridis* mutants are both pale green, but differ in *chlorina* being
viable. The *tigrina* and *striata* mutants are stripped transverse and
along the leaves, respectively.

Although the mutations are generally lethal, the large endosperm of
barley seeds supports plant growth for several weeks, allowing analysis
of the mutants at a seedling stage. This has been utilized in three
studies concerning cold acclimation (Svensson *et al*. 2006),
photosystem II (Morosinotto *et al*. 2006) and dominance/recessivity in
chlorophyll biosynthesis (Axelsson *et al*. 2006), respectively.

Svensson and collaborators (2006) used the Affymetrix Barley1 GeneChip
in combination with *albina-e*.16, *albina-f*.17, *xantha-s*.46 and
*xantha-b*.12 to assess the effect of the chloroplast on the expression
of cold-regulated genes. About 67% of wild-type cold-regulated genes
were not regulated by cold in any mutant (chloroplast-dependent
cold-regulated genes).They found that the lack of cold regulation in the
mutants is due to the presence of signalling pathway(s) normally cold
activated in wild type but constitutively active in the mutants, as well
as to the disruption of low-temperature signalling pathway(s) due to the
absence of active chloroplasts. They also found that photooxidative
stress signalling pathway is constitutively active in the mutants. These
results demonstrate the major role of the chloroplast in the control of
the molecular adaptation to cold.

The barley mutant *viridis-zb*.63 lacks photosystem I and was employed
by Morosinotto *et al*. (2006) to mimic extreme and chronic
overexcitation of photosystem II. The mutation was shown to reduce the
photosystem II antenna to a minimal size of about 100 chlorophylls per
photosystem II reaction centre, which was not further reducible. The
minimal photosystem II unit was found to consist of a dimeric
photosystem II reaction centre core surrounded by monomeric Lhcb4
(chlorophyll protein 29), Lhcb5 (chlorophyll protein 26) and trimeric
light-harvesting complex II antenna proteins. This minimal photosystem
II unit forms arrays *in vivo*, possibly to increase the efficiency of
energy distribution and provide photoprotection. In wild-type plants, an
additional antenna protein, chlorophyll protein 24 (Lhcb6), which is not
expressed in *viridis-zb*.63, is proposed to associate to this minimal
unit and stabilize larger antenna systems when needed. The analysis of
the mutant also revealed the presence of two distinct signalling
pathways activated by excess light absorbed by photosystem II: one,
dependent on the redox state of the electron transport chain, is
involved in the regulation of antenna size, and the second, more
directly linked to the level of photoinhibitory stress perceived by the
cell, participates in regulating carotenoid biosynthesis.

Axelsson *et al*. (2006) studied the enzyme Mg-chelatase, which
catalyzes the insertion of Mg^2+^ into protoporphyrin IX at the first
committed step of the chlorophyll biosynthetic pathway. It consists of
three subunits; I, D and H. The I-subunit belongs to the AAA-protein
superfamily ([A]{.underline}TPases [a]{.underline}ssociated with various
cellular [a]{.underline}ctivities) that is known to form hexameric ring
structures in an ATP-dependant fashion. Dominant mutations in the
*Xantha-h* gene, encoding the I-subunit, revealed that it functions in a
cooperative manner. Axelsson *et al*. demonstrated that the D-subunit,
encoded by *Xantha-g*, forms ATP-independent oligomeric structures and
should also be classified as an AAA-protein. Furthermore, the question
of cooperativity of the D-subunit was addressed by characterizing
*xantha-g*.28, *-g*.37, *-g*.44, *-g*.45 and *-g*.65 at the molecular
level. The recessive behavior *in vivo* was explained by the absence of
mutant proteins in the barley cell. The identified mutations were
constructed in the corresponding gene of *Rhodobacter capsulatus* and
the resulting D-proteins were studied *in vitro*. Mixtures of wild-type
and mutant *R. capsulatus* D-subunits showed a lower activity as
compared to wild-type subunits assayed alone. Thus, the mutant
D-subunits displayed a dominant behavior *in vitro* thus revealing
cooperativity between the D-subunits in the oligomeric state. Based on
these results, they proposed a model where the D-oligomer forms a
platform for the stepwise assembly of the I-subunits. The cooperative
behavior suggests that the D-oligomer takes an active part in the
conformational dynamics between the subunits of the enzyme.

The stock list of barley mutants defective in chlorophyll biosynthesis
and chloroplast development is found elsewhere in this issue of BGN and
at

<http://www.mps.lu.se/fileadmin/mps/People/Hansson/Barley_mutants_web.pdf>

Dr. Mats Hansson

Department of Biochemistry

Lund University

Box 124

SE-22100 Lund, SWEDEN

Phone: +46-46-222 0105

Fax: +46-46-222 4534

E-mail: <Mats.Hansson@biochemistry.lu.se>

**New references:**

**Axelsson, E., A. Sawicki, S. Nilsson, I. Schröder, S. Al-Karadaghi, R.
D. Willows and M. Hansson.** **2006.** Recessiveness and dominance in
barley mutants deficient in Mg-chelatase subunit D, an AAA protein
involved in chlorophyll biosynthesis. Plant Cell 18: 3606-3616.

**Morosinotto, T., R. Bassi, S. Frigerio, G. Finazzi, E. Morris and J.
Barber. 2006**. Biochemical and structural analyses of a higher plant
photosystem II supercomplex of a photosystem I-less mutant of barley.
Consequences of a chronic over-reduction of the plastoquinone pool. FEBS
J. 273: 4616-4630.

**Svensson, J.T., C. Crosatti, C. Campoli, R. Bassi, A. Michele Stanca,
T.J. Close, and L. Cativelli. 2006.** Transcriptions analysis of cold
acclimation in barley *albina* and xantha mutants. Plant Physiol. 141:
257-270.

**Coordinator's report: The Genetic Male**

**Sterile Barley Collection**

**M.C. Therrien**

**Agriculture and Agri-Food Canada**

**Brandon Research Centre**

**Box 1000A, RR#3, Brandon, MB**

**Canada R7A 5Y3**

**E-mail: [MTherrien@agr.gc.ca]{.underline}**

The GMSBC has been at Brandon since 1992. If there are any new sources
of male-sterile genes that you are aware of, please advice me, as this
would be a good time to add any new source to the collection. For a list
of the entries in the collection, simply [E-mail]{.underline} me at the
above adress. I can send the file (14Mb) in Excel format. We continue to
store the collection at -20^o^C and will have small (5 g) samples
available for the asking. Since I have not received any reports or
requests the last years, there is absolutely no summary in my report.

**Coordinator's report: Ear morphology genes.**

**Udda Lundqvist**

**Nordic Gene Bank**

**P.O. Box 41 SE-230 53 Alnarp, Sweden**

**e-mail: [udda@nordgen.org]{.underline}**

**A. Michele Stanca and Valeria Terzi**

**C.R.A., Genomic Research Centre**

**Via San Protaso 302**

**29017-Fiorenzuola d'Arda, Italy**

**e-mail: <michele@stanca.it>[; v.terzi@iol.it]{.underline}**

The studies on barley development in these last years have taken
advantages both from genetics and genomics approaches. Some barley genes
involved in the ear morphology development have been mapped on high
density molecular linkage maps and this strategy has been accompanied by
candidate gene approaches (Pozzi *et al*., 2002).

In the work of Pozzi *et al*. (2003) 29 genetic loci for which mutant
alleles exist were placed on a restriction fragment length polymorphism-
amplified fragment length polymorphism (RFLP-AFLP) map. Among the 29
loci considered in the work, some specifically affect ear morphology and
assume characteristics proper to phytomers of other regions (like *third
outer glume* and *awned palea*) or are characterized by the presence of
modified organs (like *liguleless*, *bracteatum*, *triple awned lemma*
and *awned lemma*). In Table 1 the map positions individuated by Pozzi
*et al.* (2003) for some loci involved in ear morphology are reported.

Table 1. Position of 12 developmental mutant loci in a Proctor X Nudinka
AFLP map (from Pozzi et al, 2003), but revised regarding symbols and
nomenclature.

  ----------------------- ----------------------- -----------------------
  **Mutant symbol and     **Map position**        **Closest marker**
  name**                                          

  *adp1, awned palea1*    Chr. 3H/27              E3634-8

  *als1, absent lower     Chr 3H/28               E4234-11
  laterals1*                                      

  *bra-d.7,               Chr. 1H                 E3634-7
  bracteatum-d.7*                                 

  *dub.1, double seed.1*  Chr 5H/66 and 67        E4038-4

  *hex-v.3,               Chr. 2H/19-21           E4343-7
  hexastichon-v.3*                                

  *hex-v.4,               Chr. 2H/19 and 20       E3438-3
  hexastichon-v.4*                                

  *int- c.5,              Chr. 4H/8               E4143-5
  intermedium-c.5*                                

  *Kap1, Hooded lemma1*   Chr. 4H/36 and 37       E4140-1

  *lks2, short awn2*      Chr. 7H/6               E4138-3

  *lks5, short awn5*      Chr. 4H/38              E4143-5

  *trp1, triple awned     Chr. 2H/22 and 23       E3644-13
  lemma1*                                         

  *trd1, third outher     Chr.1H/52               E3634-7
  glume1*                                         
  ----------------------- ----------------------- -----------------------

The genetics of barley *Hooded* suppression has been studied by Roig *et
al*. (2004). The genetic basis of this phenotype is a mutation in the
homeobox *Bkn3*. After chemical mutagenesis and complementation tests,
five *suK* (suppressor of K) loci were identified and mapped on
chromosomes 5H and 7H.

Comparative genetic studies across species has revealed syntenous
conservation in the order of genes and markers along grass chromosomes.
Starting from this observation, Rossini *et al.* (2006) used a synteny
approach comparing barley and rice genomes to individuate candidate
genes for a set of barley developmental mutants.

The gene *vrs1* (*six-rowed spike 1*) responsible for the six-rowed
spike in barley has been recently isolated by means of positional
cloning by Komatsuda *et al*. (2007). The wild type *Vrs1* gene, present
in two-rowed barley, encodes a transcription factor that includes a
homeodomain with a closely linked leucine zipper motif. VRS1 protein
suppresses lateral rows and give two-rowed spike, whereas a mutation in
the homeodomain-leucine zipper of *Vrs1* resulted into loss of function
and development of six-rowed phenotype.

The conservation and implementation of the barley morphological mutant
collections is essential for future studies, ranging from the use of
computer graphic L-system- models to simulate the final morphology of a
plant (Buck-Sorlin *et al.*, 2004) to the use of genomic tools for the
elucidation of the gene functions.

Regarding the Swedish mutation collection two new additional mutant loci
could be mapped based on molecular mapping studies using simple
sequences repeat (SSR) markers (Dahleen *et al*. 2005, Dahleen and
Franckowiak. 2006).

(1). The *intermedium spike-k* (*int-k*) gene could be localized in the
centromeric region of chromosome 7H, closely linked to Bmag0217 and
Bmac0162 in bins 6 to 7. This spike mutant has a short and dense spike
and the lateral spikelets are enlarged with a pointed apex. Occasionally
they have a short awn. The central spikelets are semi-sterile and there
is no seed set in the lateral spikelets. Plants have a dense coating of
wax surface. They also have significantly reduced height, peduncle
length, awn length, kernels per spike, leaf length, kernel weight and
yield.

(2). The *erectoides-t* (*ert-t*) gene, one of the dense spike mutant
loci, could be localized near the tip of chromosome 2HS, approximately
11.4 cM distal from SSR marker Bmac0134, near the boundary between bins
2H-01 and 2H-02. The spikes of this mutant gene are semicompact, rachis
internode length is about 2.7 mm and culm length is about 2/3 of normal.
These phenotypic traits plus short awns are inheritated together. Based
on general appearance of the plants, *ert-t* can be placed in the
brachytic class and by diallelic crosses three earlier identified
Brachytic 3 (*brh3*) phenotypes were found to be allelic at the *ert-t*
locus (Franckowiak, 2006).

**References**

**Buck-Sorlin, G., O. Kniemeyer, and W. Kurth. 2004.** Integrated
grammar representation of genes, metabiolites and morphology: the
example of hordeomorphs. 2004. Fourth International Workshop on
Functional Structural Plant Models, Montpellier, France pp. 386-389.

**Dahleen, L.S., L.J.Vander Val, and J.D. Franckowiak. 2005.**
Characterixation and molecular mapping of genes determining semidwarfism
in barley. J. Hered. 96:654-662.

**Dahleen, L.S., and J.D. Franckowiak. 2006.** SSR Linkages to Eight
Additional Morphological Marker Traits. Barley Genetics Newsletter (BGN)
36:12-16.

**Franckowik, J,D. 2006.** Coordinator's report: Chromosome 2H (2). BGN
36:54-56

**Komatsuda, T., M. Pourkheirandish, C. He, P. Azhaguvel, H. Kanamori,
D. Perovic, N. Stein, A. Graner, T. Wicker , A. Tagiri, U. Lundqvist, T.
Fujimura, M. Matsuoka, T. Matsumoto, and M. Yano M. 2007.** Six-rowed
barley originated from a mutation in a homeodomain-leucine zipper
I-class homeobox gene. PNAS 104: 1424-1429.

**Pozzi, C., L. Rossini, L. Santi, M.R. Stile, L. Nicoloso, F. Barale,
D. Vandoni, I. Decimo, C. Roig, P. Faccioli, V. Terzi, Y. Wang, and F.
Salamini, F. 2002.** Molecular genetics of barley development: from
genetics to genomics. In: From biodiversity to genomics: breeding
strategies for small grain cereals in the third millenium. Eucarpia
Cereal Section Meeting, 2002. pp 463- 466.

**Pozzi, C., D. Di Pietro, G. Halas, C. Roig, and F. Salamini. 2003.**
Integration of a barley (*Hordeum vulgare*) molecular linkage map with
the position of genetic loci hosting 29 developmental mutants. Heredity,
90: 390-396.

**Roig, C., C. Pozzi, L. Santi, J. Muller, Y. Wang, M.R. Stile, L.
Rossini, A.M. Stanca, and F. Salamini. 2004.** Genetics of barley
*Hooded* suppression. Genetics, 167: 439-448.

**Rossini L., A. Vecchietti, L. Nicoloso, N. Stein, S. Franzago, F.
Salamini, C. and Pozzi. 2006.** Candidate genes for barley mutants
involved in plant architecture: an *in silico* approach. Theoretical and
Applied Genetics, 112: 1073-1085.

**Coordinator's report: Semidwarf genes**

**J.D. Franckowiak**

**Hermitage Research Station**

**Queensland Department of Primary Industries and Fisheries**

**Warwick, Queensland 4370, Australia**

**e-mail: [jerome.franckowiak@dpi.qld.gpv.au]{.underline}**

The *sdw1* (*denso*) gene for the semidwarfism in barley was shown to be
orthologous to the *sd1* of rice based on similar map positions and
linkage of both semidwarf genes to RFLP maker R1545 (Zhang *et al*.,
2005). Both genes are sensitive to gibberellic acid (GA) treatments and
encode a GA~20~-oxidase mutant (*HV20ox2*), which produces lower levels
of GA and causes the dwarf phenotype. Zhang *et al*. (2005) reported
that the barley and rice genes shared 88% sequence similarity and 89%
amino acid identity.

Yin *et al*. (2005) confirmed that QTLs that lengthening the
preflowering duration in the 'Apex'/'Prisma' population of 94
recombinant inbred lines (RILs) were located in the long arms of
chromosome 2H and 3H and originated from Prisma. The QTL on 3HL was
associated with presence of the *sdw1* gene from Prisma and likely is a
pleiotropic effect of *sdw1* gene.

Korff *et al*. (2006) detected the presence of the *sdw1* gene from
'Scarlett' in doubled-haploid lines from the second backcross of
Scarlett to *Hordeum vulgare* ssp. *spontaneum* accession ISR42-8. QTLs
for plant height were detected also on other chromosomes by Korff *et
al*. (2006).

Gruszka *et al*. (2006) reported that a semidwarf mutant 093AR, which
was produced by MNU (N-methyl-N-nitrosourea) treatment of variety
Aramir, is allelic to the uzu (*uzu1* on chromosome 3HL) dwarfing gene.
Their analysis of the DNA sequence of the *HvBRI1* gene of 093AR showed
a single-nucleotide substitution of the C to A substitutions at the
positions 1760 and 1761. Gruszka et al. (2006) also confirmed that the
*uzu1* mutation was an A to G change at position 2612 of the *HvBRI1*
gene. Chono *et al*. (2003) previously reported that the mutation
resulted in an amino acid change at the highly conserved residue
(His-857 to Arg-857) of the kinase domain of BRI1 (brassinosteroids)
receptor protein. This change caused reduced sensitivity to BRs and
reduced plant height (Chono *et al*., 2003).

**References:**

**Gruszka, D., J. Zbieszczyk, M. Kwasniewski, I. Szarejko and M.
Maluszynski. 2006.** A new allele in a *uzu* gene encoding
brassinosteroid receptor. Barley Genet. Newsl 36:1-2. author:
<wintergrun@op.pl>

**Chono, M., I. Honda, H. Zeniya, K. Yoneyama, D. Saisho, K. Takeda, S.
Takatsuto, T. Hoshino and Y. Watanabe. 2003.** A semidwarf phenotype of
barley uzu results from a nucleotide substitution in the gene encoding a
putative brassinosteroid receptor. Plant Physiol. 133:1209-1219.

**von [Korff](http://www.springerlink.com/content/?Author=M.+von+Korff),
M., [H. Wang](http://www.springerlink.com/content/?Author=H.+Wang), [J.
Léon](http://www.springerlink.com/content/?Author=J.+Léon), and [K.
Pillen](http://www.springerlink.com/content/?Author=K.+Pillen). 2006.**
[AB-QTL analysis in spring barley: II. Detection of favourable exotic
alleles for agronomic traits introgressed from wild barley (*H. vulgare*
ssp.
*spontaneum*)](http://www.springerlink.com/content/y331x8755051h71j/?p=8e7dfcb66bd04c5f9c568364a28f27c8&pi=2).
Theor. Appl. Genet. 112:1221-1231.

**Yin, X., P.C. Struik, F.A. van Eeuwijk, P. Stam, and J. Tang. 2005.**
[QTL analysis and QTL-based prediction of flowering phenology in
recombinant inbred lines of
barley](http://proquest.umi.com/pqdweb?index=18&did=818458691&SrchMode=3&sid=2&Fmt=10&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1183339531&clientId=62902&aid=1)
J. Exp. Bot. 56(413):967-976.

**Zhang, J., X. Yang, P. Moolhuijzen, C. Li, M. Bellgard, R. Lance, and
R. Appels. 2005.** Towards isolation of the barley green revolution
gene. Australian Barley Technical Symposium 2005.
[http://www.cdesign.com.au/proceedings_abts2005/posters%20(pdf)/poster_li.pdf](http://www.cdesign.com.au/proceedings_abts2005/posters (pdf)/poster_li.pdf).
Verified 4 July 2007.

# Coordinator's report : Wheat-barley genetic stocks

## A.K.M.R. Islam

**Faculty of Agriculture, Food & Wine, The University of Adelaide, Waite
Campus,**

## Glen Osmond, SA 5064, Australia

**e-mail: [rislam@waite.adelaide.edu.com]{.underline}**

The production of five different disomic addition lines (1Hm, 2Hm, 4Hm,
5Hm and 7Hm) of *Hordeum marinum* chromosomes to Chinese Spring wheat
has been reported earlier. It has now been possible to isolate a
monosomic addition for chromosome 6Hm. Amphiploids have also been
produced between *H. marinum* and more cultivars of commercial wheat
(Islam and Colmer, unpublished).

**References:**

**Islam,S; Malik, AI; Islam, AKMR; Colmer TD. 2007.** Salt tolerance in
a *Hordeum marinum-Triticum aestivum* amphiploid, and its parents. J
Experimental Botany (in Press).

Coordinator's report: Early maturity genes

**Udda Lundqvist**

**Nordic Gene Bank**

**P-O. Box 41**

**SE-23 053 Alnarp, Sweden**

**e-mail: udda@nordgen.org**

Not much new research on gene localization has been reported on the
Early maturity or Praematurum genes since the latest reports in Barley
Genetic Newsletter (BGN) or in the AceDB database for Barley Genes and
Barley Genetic Stocks.

During the last years many reports are published on various QTLs
detected in populations derived from wild x cultivated barley crosses
with the goal of transferring desirable genes into cultivated barley
lines.

Korff *et al*. (2006) transferred favourable genes from wild barley to
cultivated barleys and made evaluations in backcrosses of a doubled
haploid population. A QTL for Early heading was associated with the
Early maturity 1 (*Eam1* or *Ppd-H1*) gene in the bin 3 region of 2HS.

Several QTLs were found in crosses between two- and six-rowed cultivars.
One QTL for early heading is reported and found in bin 8 of 2HL and is
probably the *Eam6* gene from a six-rowed parent (Franckowiak 2006).

All information and descriptions made in the Barley Genetics Newsletter
are valid and up-to-date. As my possibilities in searching literature
are very limited, I apologize if I am missing any important papers and
reports. I would like to call on the barley community to assist me by
sending notes of publications and reports to include in next year's
report. Descriptions, images and graphic chromosome map displays of the
Early maturity or Praematurum genes are available in the AceDB database
for Barley Genes and Barley Genetic Stocks. They get currently updated
and are searchable under the address: [www.untamo.net/bgs]{.underline}

Every research of interest in the field of Early maturity genes can be
reported to the coordinator as well. Seed requests regarding the Swedish
mutants can be forwarded to the coordinator or directly to the Nordic
Gene Bank, [www.nordgen.org/ngb]{.underline}, all others to the Small
Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA,
<nsgchb@ars-grin.gov> or to the coordinator at any time.

**References:**

**Franckowiak, J.D. 2006.** Coordinator's report: Chromosome 2H. Barley
Genetics Newsletter 36:54-56.

**Korff, M. von, H. Wang, J. Léon, and K. Pilen. 2006.** AB-QTL analysis
in spring barley: II. Detection of favourable exotic alleles for
agronomic traits introgressed from wild barley (*Hordeum vulgare ssp.
spontaneum*). Theor. Appl. Genet. 112:1221-1231.