National Marine Fisheries Service (NMFS)\
Application Instructions for a Permit for Scientific Purposes\
or to Enhance the Propagation or Survival of Threatened and Endangered
Species

OMB control number: 0648-0402 Expiration date: 02/28/2009

**Authority**

Under section 10(a)(1)(A) of the Endangered Species Act of 1973 (ESA),
NMFS may issue permits for scientific research purposes or to enhance
the propagation or survival of species listed as threatened or
endangered under the ESA. The authorization provided by these permits
exempts the permit holder from the prohibitions of ESA section 9, in
particular those dealing with take. *Take* is defined by the ESA as:
\"to harass, harm, pursue, hunt, shoot, wound, kill, trap, capture, or
collect, or to attempt to engage in any such conduct.\" Permitted
activities must not operate to the disadvantage of the listed species
and must provide a bona fide and necessary or desirable scientific
purpose or enhance the propagation or survival of the listed species.
NMFS traditionally issues permits for up to five years, although permits
for longer periods of time have been issued. Permits include any
conditions necessary to mitigate and monitor the impacts of the proposed
activities. These application instructions are drawn from, but do not
substitute for, the ESA section 10 regulations at 50 CFR §222.308.
Applicable state laws or regulations prevail in all cases where they are
more restrictive. Possession of a section 10(a)(1)(A) permit should be
regarded as a privilege in that NMFS must balance permit issuance with
its duties to protect and recover listed species.

**Do I Need to Apply for a Permit?**

If you determine that your proposed activity would *directly take* a
listed species (i.e., the listed species is the subject of your proposed
activity, and conducting it would likely result in the species being
harassed, captured, harmed, possessed, or killed) and your planned
activity is otherwise lawful, a section 10 (a)(1)(A) permit is required.
Examples of activities that may require a section 10 (a)(1)(A) permit
include: surveys, genetic research, hatchery operations, relocations,
capture and marking, and telemetric monitoring. Under certain
circumstances, a section 10(a)(1)(A) permit may also be required for you
to possess listed species' tissues or body parts.

If you are engaged in an otherwise lawful activity where a listed
species may be adversely affected, and the purpose of your activity is
not scientific research or species enhancement, you may need to obtain a
section 10(a)(1)(B) permit (*Incidental Take Permit*). Examples of
activities that may require a section 10(a)(1)(B) permit include: state
sportfishing programs, non-listed fish stocking programs, and other
instream or watershed activities which may affect listed species. If
your proposed activities require an incidental take permit, you will
need to use the

section 10(a)(1)(B) instructions. These are available at the addresses
listed below under *Where Do I Send the Application?* and at the NMFS
Office of Protected Resources web site,

<http://www.nmfs.noaa.gov/pr/permits/esa_permits.htm>

If you still have questions, you may want to consult the Pre-Application
Guide (PAG) on the Authorizations and Permits for Protected Species
website. The PAG will walk you through a series of questions to help you
determine what type of permit you need and whom you should contact. The
PAG can be found at <http://apps.nmfs.noaa.gov/>.

Before applying for an individual permit, you should determine if your
proposed project is a part of another authorized activity. To minimize
duplication---and the impact on listed species---you are strongly
encouraged to coordinate with others doing similar work. If two
investigators are collaborating on the same activities, they should
apply for a single permit. Also, if you are conducting your proposed
activities in response to a Federal Agency requirement, you may not need
a section 10 (a)(1)(A) permit. For example, fish surveys required by an
ESA section 7 biological opinion may be authorized in the opinion's
Incidental Take Statement and no section 10 permit would be needed.

**Am I Using the Appropriate Application Instructions?**

These instructions are for permits for research and/or enhancement
activities involving listed Pacific salmon and steelhead. They can be
downloaded from: <http://www.nmfs.noaa.gov/pr/permits/esa_permits.htm>

Permits for marine mammal species and non-salmonid threatened and
endangered species (e.g., shortnose sturgeon, sea turtles, white
abalone), please use the application instructions available at:
[http://www.nmfs.noaa.gov/pr/permits/](http://www.nmfs.noaa.gov/pr/permits/mmpa_permits.htm)

For terrestrial or freshwater species, or land-based sea turtle
activities, please contact the appropriate regional office of the U.S.
Fish and Wildlife Service:
[[http://www.fws.gov/r9irmtsb/regional.html]{.underline}](http://www.fws.gov/r9irmtsb/regional.html)

**When Should I Apply?**

To allow for processing time, you are urged to apply at least six months
before you need to start your proposed activities, though certain permit
actions may take even longer to process.

**What Should I Include in the Application?**

A permit application should provide all of the information requested
below and, for processing efficiency, it should be displayed in the same
structure and format. We will use the information that you provide to
determine whether your application is complete and whether to issue a
permit for the proposed activities. If a section does not apply to your
activities, please do not skip the section, simply mark it \"not
applicable\" or \"N/A.\" Applicable information should be detailed
enough to provide a complete picture of your proposed activities.
Incomplete or vague information will delay processing. Please note that
specific wording is required for the title and closing statement. If you
already have a project proposal, you may attach the proposal and
reference the appropriate sections of it when filling out your
application.

**Should I Send a Review Draft?**

It is often helpful to draft an application and send it to us for review
before mailing your final application. Send the draft electronically
(contact the appropriate office for a current email address). Our staff
will review your application and help you if there are any difficulties.
Once the application is complete, you must send a signed copy to the
appropriate office.

**Where Do I Send the Application?**

Mail one signed original of the complete, final application to the
appropriate address below. You should also submit a copy electronically
(on disk or by e-mail) to help speed processing. If you need help
completing your application, submit a draft to the appropriate office or
contact them directly.

[Permits for marine and anadromous species in the Pacific
Northwest]{.underline}:

Chief, Protected Resources Division

> National Marine Fisheries Service - F/NWO3
>
> 1201 NE Lloyd Boulevard, Suite 1100
>
> Portland, Oregon 97232-1274
>
> Phone: 503-230-5400
>
> Fax: 503-230-5441

[Permits for marine and anadromous species in California]{.underline}:

-   NMFS Northern California Coast Salmon\
    Supervisor, Protected Resources Division\
    National Marine Fisheries Service\
    1655 Heindon Road\
    Arcata, CA 95521\
    Phone: (707) 825-5163  Fax: (707) 825-4840

-   NMFS Central California Coast Salmon\
    Supervisor, Protected Resources Division\
    National Marine Fisheries Service\
    777 Sonoma Ave., Room 325\
    Santa Rosa, CA 95404\
    Phone: (707) 575-6050   Fax: (707) 578-3435

-   NMFS California Central Valley Salmon\
    Supervisor, Protected Resources Division\
    National Marine Fisheries Service\
    650 Capitol Mall, Suite 8-300\
    Sacramento, CA 95819\
    Phone: (916) 930-3600   Fax: (916) 930-3629

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-   NMFS Southern California Salmon\
    Supervisor, Protected Resources Division\
    National Marine Fisheries Service\
    501 West Ocean Blvd\
    Long Beach, CA 90802-4250\
    Phone: (562) 980-4020   Fax: (562) 980-4027

    **What is Involved in Processing a Permit Application?**

Once we receive a *complete* permit application, it is subject to a
30-day public comment period. The 30-day public comment period begins
when a \"notice of receipt\" is published in the *Federal Register*
(required by regulations). We may also distribute the application for
review by scientific and technical experts, resource managers, and/or
other Permit Holders. After the 30-day public comment period, we will
forward a summary of the comments for the applicant to address. The
applicant must submit a written response.

We must then conduct an ESA section 7 consultation on the proposed
activity. This, in turn, results in a biological opinion on the
activity. To issue a section 10(a)(1)(A) permit for any activity, NMFS\'
biological opinion must conclude that the proposed activity is not
likely to jeopardize the continued existence of any ESA-listed species
nor destroy nor adversely modify any species' designated critical
habitat.

In addition, we may be required to conduct the following consultations
on the potential effects of the activity proposed in the application:
(1) Consultation with U.S. Fish and Wildlife Service regarding potential
effects on species under their jurisdiction, (2) consultation between
NMFS' Protected Species Division and NMFS' Habitat Conservation Division
regarding any activities taking place in Essential Fish Habitat (EFH),
and (3) consultation with the National Ocean Service if the action takes
place in a National Marine Sanctuary. Any issues that arise during these
consultations may delay the permit process.

Finally, we may be required to conduct an analysis under the National
Environmental Policy Act (NEPA) if a proposed activity is one designed
to enhance the propagation and/or survival of an ESA-listed species
(e.g., hatchery supplementation programs or fish salvage operations).
That analysis usually consists of an Environmental Assessment (EA) on
the action of issuing a section 10(a)(1)(A) permit with conditions. In
general, scientific research permits are categorically excluded from the
need to conduct an analysis under NEPA.

After we receive responses from the applicant to the public comments and
complete a biological opinion (and any other required consultations), we
will decide whether or not to issue the permit. In order to issue such a
permit, we must find that it: (1) Was applied for in good faith, (2) if
granted and exercised will not operate to the disadvantage of listed
species subject to the permit, and (3) will be consistent with the
purposes and policies set forth in the ESA. We will then notify the
applicant of the decision and publish a notice about the decision in the
*Federal Register* (required by regulations).

**How Do I Modify an Existing Permit?**

A request to modify an existing permit should address all sections of
these instructions relevant to the requested change and include a
detailed description and justification of the proposed changes.
Modification requests involving an increased number of animals,
increased risk to the species, additional listed species, or significant
changes in the nature or location of activities may be subject to a
30-day public review period and reconsultation on the effects of issuing
the modified permit.

**Paperwork Reduction Act and Confidentiality Information**

An applicant must submit the information requested in the following
instructions to obtain an ESA section 10(a)(1)(A) permit. We will use
that information to process the permit request in accordance with the
ESA. The information provided is not confidential and is subject to
public review and comment.

Notwithstanding any other provision of the law, no person is required to
respond to, nor shall any person be subject to a penalty for failure to
comply with, a collection of information subject to the requirements of
the Paperwork Reduction Act, unless that collection of information
displays a currently valid OMB (Office of Management and Budget) Control
Number. In addition, persons are not required to retain records for more
than three years unless those records are health, medical, government
contract, grant-in-aid, or tax records.

NMFS estimates that the average time to compile an application in
accordance with these instructions is 30 hours. This includes the time
needed to review instructions, search existing data sources, gather and
maintain the data needed, and complete and review the application. For
annual permit reports, NMFS estimates average response time at 10 hours
per report. You may send comments regarding these estimates or any other
aspect of this information collection, including suggestions for
reducing this burden, to the addresses under *Where Do I Send the
Application?*

**Information to Include in the Application**

A.  **Title**: Use one of the titles below, exactly as worded, and
    provide a brief Project Name.

**Title**

1.  Application for Permit for Scientific Purposes under the Endangered
    Species Act of 1973. (If the proposal is for field surveys, genetics
    research, etc.)

2.  Application for Permit to Enhance the Propagation or Survival of
    Listed Species under the Endangered Species Act of 1973. (If the
    proposal is for fish hatchery operations, etc.)

3.  Application for Permit for Scientific Purposes and to Enhance the
    Propagation or Survival of Listed Species Under the Endangered
    Species Act of 1973. (If the proposal is for activities that fall
    under both categories 1 and 2 above)

4.  Application for Modification of Existing Permit.

> **Project Name**
>
> Describe the project in 20 words or less (e.g., Salmonid Use of
> Restored Estuarine Wetlands: Regional Application of the Salmon River,
> Oregon, Estuary Study).

B.  **Species**: List all species and Evolutionarily Significant Units
    (ESUs) and/or populations for which you request take authority.

C.  **Date of Permit Application**: Date you are sending the
    application. If you submit subsequent versions of the application
    (after you receive guidance from NMFS) update the dates to reflect
    the date the updated version is sent.

D.  **Applicant Identity**: The applicant is the individual and/or
    agency responsible for ensuring compliance with permit conditions,
    and may represent a group of individuals actually performing the
    activities (e.g., employees, partners, agents, and/or contractors).
    Please include the following information about the permit applicant:

    1.  Applicant's name and position title

    2.  Institution or agency name

    3.  Mailing address

    4.  Telephone number

    5.  Fax number

    6.  Email address

> If NMFS should be coordinating with a contact person different from
> the applicant, also include the same information (1-6 above) for the
> principal contact. Attach résumés, if any, at the end of the
> application or provide them in a separate document.

E.  **Information on Personnel, Cooperators, and Sponsors**: If the same
    person or entity will hold several roles, you may state their
    address information once and refer back to it.

    1.  If the applicant will not be the sole person conducting the
        proposed activities, provide the names, email addresses, phone
        numbers, and résumés for each Principal Investigator and Field
        Supervisor. A [Principal Investigator]{.underline} is ultimately
        responsible for the project and compliance with the permit
        conditions. A [Field Supervisor]{.underline} (who may also be
        the Principal Investigator), is anyone who supervises or carries
        out the activities in the field without supervision, and will
        also be responsible for compliance with the permit conditions.
        Attach résumés, if any, at the end of the application or provide
        them in a separate document.

    2.  To the extent possible, provide a list of field personnel.

    3.  Provide the name, title, agency, phone number, and any other
        appropriate contact information for all sponsors, cooperating
        institutions, etc.

    4.  If the proposed activities will be conducted by a contractor,
        provide a statement that a qualified member of your staff
        (include name(s) and qualifications) will supervise or observe
        the taking. Include a copy of the proposed contract or a letter
        from the contractor indicating agreement to operate under any
        and all permit conditions, should a permit be granted.

    5.  Provide a description of the arrangements for the disposition of
        any tissue samples, dead specimens, or other remains. If you
        will not retain samples, state that samples will be returned to
        their capture site (see section H.2.). If you are going to
        retain tissue samples (including whole fish), either in a museum
        or other institution for the continued benefit to science,
        include information on where the samples will be stored,
        transferred, and how/when/where they will be disposed. Include
        the list of researchers, laboratories, museums, and/or
        institutional collections that would receive these tissue
        samples or specimens. Please include name, address, contact, and
        phone number for each.

    6.  For transport and long-term holding of listed species (see
        Section I), provide the qualifications and experience of all
        staff responsible for care without supervision, including a
        written certification from a licensed veterinarian knowledgeable
        about the requested species (or similar species), or from a
        recognized expert on the species (or similar species) that
        he/she has personally reviewed the criteria for transporting and
        maintaining the animal(s) and that in his/her opinion they are
        adequate to provide for the well-being of the animal. Include
        the name, address, email, and phone number of this veterinarian,
        consulting expert, or equivalent who will be available during
        the proposed activities.

F.  **Project Description, Purpose, and Significance**: Describe the
    purpose of your study or project. If available, attach a copy of the
    formal project proposal or contract, including the contract number,
    to your application. You may reference the appropriate section of
    the proposal/contract in response to a particular question.

    1.  A justification of the objective(s): motivation, history, goals,
        etc. State how the listed species will benefit from the proposed
        activities. Begin with a brief description of the purpose of the
        research (30 words or less). *For example: The purposes of the
        research are to assess watershed conditions and factors limiting
        salmonid health and production, and evaluate watershed health
        under the Northwest Forest Plan.*

    2.  A statement of whether or not the proposed project or program
        responds directly or indirectly to a recommendation or
        requirement of a Federal agency (Include citations if
        applicable). Identify any secured or proposed Federal funding
        source(s) for the proposed activities, including names,
        addresses, and phone numbers of the sponsors, cooperating
        institutions, etc.;

    3.  A statement of whether or not the proposed project or program
        has broader significance than the individual project\'s goals,
        or is part of a larger scale research management or restoration
        plan (Include citations if applicable);

    4.  A description of any relationships or similarities of the
        proposed activities to other proposed or ongoing projects and
        programs, and whether the potential exists to cooperate and
        coordinate with other similar studies or activities. (Include
        citations if applicable); and

    5.  A justification for using listed species in the study or
        activities, and a discussion of possible alternatives to using
        listed species.

G.  **Project Methodology**: Provide a detailed description of the
    project, or program, in which the listed species is to be used,
    including:

    1.  The proposed duration of the project or program, including start
        and end dates. Provide the date the project is scheduled to
        start. Be realistic. Use a start date, whenever possible, that
        is at least six months after the application is submitted. Use
        an end date that covers all possible scenarios, or up to five
        years from the start date. If the project or program will
        continue into the foreseeable future, provide the rationale for
        the extended time frame. Provide times during the year that
        specific activities will occur. Be specific if multiple
        activities are proposed (e.g., spawning surveys from October to
        December; juvenile trapping from April to June).

    2.  A discussion of the procedures and techniques which will be used
        during the project. Begin with a BRIEF description of the
        capture methods (seine, backpack electrofishing, etc.) and a
        brief description of any Aintrusive methods@ (anesthetic,
        tagging, marking, tissue samples, etc.). *For example: Listed
        fish will be captured (using boat electrofishing, fyke nets, and
        minnow traps), anesthetized, measured, checked for tags, marked,
        sampled for stomach content, and released.* Follow with more
        specific descriptions that will allow us to assess the
        activities. The discussion should include, at a minimum:

        a.  Method(s) of capture and of release;

        b.  The sampling schedule, including locations and dates if
            available. Be as specific as possible. Locations should be
            listed from general to most specific, including bodies of
            water, rivers, tributaries, streams or creeks, and a
            geographical descriptor (e.g., Columbia River, Snake River,
            Imnaha River, River Mile 42). Include latitude/longitude
            coordinates, if possible. Include 4^th^ field hydrologic
            units (HUCs) whenever possible.

        c.  A description of any tags, including the attachment method,
            location, and special handling/holding associated with the
            tagging;

        d.  A description of type and dosage of any drugs to be used,
            purpose of use, and method of application;

        e.  Temporary holding time prior to release of the individual(s)
            and the manner in which they will be detained. For transport
            and long-term holding, please fill out section I
            *Transportation and Holding*; and

        f.  Number and types of samples to be taken from each
            individual, including sampling protocol.

    3.  A discussion of possible alternatives to using the proposed
        methods. If applicable, you should try to anticipate alternative
        scenarios due to circumstances such as changes in environmental
        conditions, annual variations in species abundance, necessary
        changes in proposed procedures, etc. Such scenarios should be
        addressed in the *Description and Estimates of Take* section
        below if they affect the nature or amount of potential take of
        listed species. This planning may avoid the potentially lengthy
        process of modifying the permit.

    4.  A discussion of the potential for injury or mortality to the
        species involved, and the steps that will be taken to minimize
        adverse effects and to ensure that the species will be taken in
        a humane manner.

H.  **Description and Estimates of Take**: Issued permits define a
    specific number of individuals of each species that can be taken
    under the approved study or project. You must provide sufficient
    detail in the attached table (see last page) for NMFS to determine
    the species, population group, and estimated number of individuals
    to be taken by each activity. You should also describe the specific
    life stage, and origin, (and sex, if appropriate) of the listed
    species targeted. Take into account alternative scenarios identified
    above in the *Project Description, Purpose, and Significance*
    section.

> Provide a separate table for each project, activity, or location, if
> appropriate. Attach the table at the end of the application. In
> addition, include:

1.  Describe the recent status and trends of each ESU/species proposed
    to be taken (include citations where possible). NMFS already
    possesses information at the ESU level (see various NMFS web sites),
    so there is no need to repeat it in your application. We are seeking
    new data here---specifically, status and trend data on any distinct
    populations the proposed action is likely to affect. Such
    information will help us evaluate the probable impacts of the
    proposed research.

2.  Provide a justification for all potential mortalities by take
    category. You should explain how you determined the numbers of
    listed species that would be killed, either intentionally (direct
    mortality, lethal take) or unintentionally (indirect mortality). You
    may reference section G.4. in explaining mortality rates.

3.  Provide details on how all take estimates, including mortalities,
    were derived. Include citations when applicable.

4.  Include a statement as to whether or not any USFWS listed species
    would be affected. If any would be, include which species and DPS'
    and the authority you have to take those species (permit,
    consultation, agreement).

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I.  **Transportation and Holding**

    1.  **Transportation of a Listed Species**: Provide a description of
        how any live individuals taken from the capture site or other
        facility (including rescue and relocation activities) will be
        transported including:

        a.  Mode of transportation and name of transportation company,
            if applicable.

        b.  Length of time in transit for the transfer of the
            individual(s) from the capture site to the holding facility
            or to the target location.

        c.  Length of time in transit for any planned future
            move/transfer of the individual(s).

        d.  The qualifications of the common carrier or agent used for
            transportation of the individual(s).

        e.  A description of the tank, container, or other devices used
            both to hold the individual(s) at the capture site and
            during transportation.

        f.  Special care before, during and after transportation (e.g.,
            use of oxygen, temperature control, anesthetics,
            antibiotics, etc.)

    2.  **Holding of a Listed Species**: Describe the plan for care and
        maintenance of any live individuals, including a complete
        description of the facilities where any such individuals will be
        maintained including:

        a.  The dimensions of the tank(s) or other holding facilities
            and the number of individuals, by species, life stage, and
            origin, to be held in each.

        b.  The water supply, amount, and quality, including controls on
            temperature and dissolved oxygen.

        c.  The amount and type of diet used for all individuals, and
            food storage.

        d.  Sanitation practices used.

    3.  **Emergency contingencies**: Identify emergency contingencies-
        e.g., backup life support systems, alarm systems, redundant
        water and oxygen supply, release or destroy decision chains,
        etc.

J.  **Cooperative Breeding Program:** You MUST include a statement of
    willingness to participate in a cooperative breeding program and to
    maintain or contribute data to a breeding program, if such action is
    requested.

K.  **Previous or Concurrent Activities Involving Listed Species**:

    1.  Identify all previous permits where you were the permit holder
        or primary investigator working with federally-listed species;
        identify which species.

    2.  For the above permits, list all mortality events of listed
        species that have occurred in the last five years.

        a.  List the ESU/species, life stage, origin, and population
            where applicable;

        b.  Describe the number and causes of mortalities; and

        c.  Describe the measures that have been taken to diminish or
            eliminate such mortalities, and the effectiveness of those
            measures.

L.  **Certification**: You must include the following paragraph, exactly
    as worded, followed by the applicant or responsible party\'s
    signature, name, position title, and date:

> \"I hereby certify that the foregoing information is complete, true
> and correct to the best of my knowledge and belief. I understand this
> information is submitted for the purpose of obtaining a permit under
> the Endangered Species Act of 1973 (ESA) and regulations promulgated
> thereunder, and that any false statement may subject me to the
> criminal penalties of 18 U.S.C. 1001, or to penalties under the ESA.\"

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

> Signature Date

\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Name and Position Title (print)

Attach résumés here or submit it/them as a separate document.

M.  **Length of Time and Cost to Prepare Application (Optional)**: The
    public burden of these application instructions is evaluated
    periodically by the Office of Management and Budget under the
    Paperwork Reduction Act. Your response will help improve the
    accuracy of the estimates given for evaluation. You may send
    comments regarding this estimate or any other aspect of this
    information collection, including suggestions for reducing this
    burden, to the Chief, Endangered Species Division, at the address
    under *Where Do I Send the Application?*

    1.  Please estimate the length of time, in hours, it took to compile
        this application.

    2.  Please estimate the cost, in \$US, of compiling this
        application, excluding the labor hours identified in 1. above.
        This estimate should include: cost of paper, printing, mailing,
        photocopying, etc.

**[Anticipated Annual Take]{.underline}**

Use this table to specify anticipated types and numerical estimates of
annual take for listed species during individual research or enhancement
activities. Use a separate table for each discrete project or location
**and label tables accordingly**. Each row must be explained in the
application. All mortalities must be justified.

Location/Project:\_[(if
needed)]{.underline}\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

+-------+-----+-------+---------+-------+---------+-------+-------+
| **E   | **L | **Ori | **Take  | **N   | **Re    | **Res | **Res |
| SU/** | ife | gin** | Act     | umber | quested | earch | earch |
|       | S   |       | ivity** | of    | U       | Locat | Per   |
| **Sp  | tag |       |         | Fish  | nintent | ion** | iod** |
| ecies | e** |       |         | R     | ional** |       |       |
| and   |     |       |         | eques |         |       |       |
| popul |     |       |         | ted** | **Mort  |       |       |
| ation |     |       |         |       | ality** |       |       |
| group |     |       |         |       |         |       |       |
| if    |     |       |         |       |         |       |       |
| app   |     |       |         |       |         |       |       |
| ropri |     |       |         |       |         |       |       |
| ate** |     |       |         |       |         |       |       |
+-------+-----+-------+---------+-------+---------+-------+-------+
|       |     |       |         |       |         |       |       |
+-------+-----+-------+---------+-------+---------+-------+-------+
|       |     |       |         |       |         |       |       |
+-------+-----+-------+---------+-------+---------+-------+-------+
| **    | Ju  | Na    | C       | 1,000 | 3       | Lake  | May-A |
| Examp | ven | tural | apture, |       | 0/1,000 | Washi | ugust |
| le:** | ile |       | handle, |       |         | ngton |       |
|       |     |       | PIT     |       |         |       |       |
| PS    |     |       | tag,    |       |         |       |       |
| Ch    |     |       | release |       |         |       |       |
| inook |     |       |         |       |         |       |       |
| S     |     |       |         |       |         |       |       |
| almon |     |       |         |       |         |       |       |
+-------+-----+-------+---------+-------+---------+-------+-------+
| **    | Ju  | L     | Inte    | 50    | N/A     | Sout  | June  |
| Examp | ven | isted | ntional |       |         | hwest |       |
| le:** | ile | Hat   | mo      |       |         | Washi |       |
|       |     | chery | rtality |       |         | ngton |       |
| PS    |     | Cl    |         |       |         |       |       |
| Ch    |     | ipped |         |       |         |       |       |
| inook |     | Ad    |         |       |         |       |       |
| S     |     | ipose |         |       |         |       |       |
| almon |     |       |         |       |         |       |       |
+-------+-----+-------+---------+-------+---------+-------+-------+

**ESU/Species:** List each ESU and Species (and populations, if
appropriate) you are requesting to take. Include common and scientific
names.

**Life Stage:** Specify fry, juvenile, smolt, pre-spawned adult,
post-spawned adult (also note if live or dead when captured). You may
combine juvenile (fry, juvenile, smolt) life stages.

**Origin:** Specify if the individuals are natural (wild), listed
hatchery with intact adipose fins, or listed hatchery with clipped
adipose fins. Do not include unlisted hatchery fish.

**Take Activity:** Specify only one of the following for each line:

Collect for transport (including rescue/salvage)

> Capture, handle, release
>
> Capture, handle, tag, mark, tissue sample, and/or other invasive
> procedure, release
>
> (Enter one or more intrusive procedure; you may combine or split.)
>
> Intentional mortality (lethal take, direct mortality)
>
> Removal (e.g., for broodstock collection)
>
> Other take (specify)

**Number of Fish Requested:** Enter the number of fish that you are
requesting for each Take Activity.

**Requested Unintentional Mortality:** Enter the number of fish that
might die as an unintended result of the Take Activity. Enter it as a
number OUT OF the number of fish requested for each Take Activity. Use
N/A when Take Activity = Intentional mortality.

**Research Location:** Enter a location for each take. Identify
locations that are more specific than whole project. Enter to the 4^th^
field hydrologic unit code (HUC) whenever possible.

**Research Period:** Enter a range of dates. Identify dates if more
specific than project as a whole.