[CURRICULUM VITAE]{.underline}

[Name]{.underline}: **Xue-Ming Xu**, M.D. & Ph.D.

[Education]{.underline}**:**

1995 - 1998 **Ph. D.**, Dept. of Bioscience & Biotechnology, Tokyo
Institute of Technology,

> Tokyo, Japan.

1992 - 1995 **MSc.**, Dept. of Biochemistry & Molecular Biology, Hunan
Medical University,

> Hunan, China.

1982 - 1987 **M. D.**, Hunan Medical University, Hunan, China.

[Brief Chronology of Employment]{.underline}:

2004 - Present **Staff Scientist**, Molecular Biology of Selenium
Section, Laboratory of Cancer Prevention, NCI, Bethesda, MD

2003 - 2004 **Senior Staff Fellow**, Molecular Biology of Selenium
Section, Laboratory of Cancer Prevention, NCI, Bethesda, MD.

2001 - 2003 **Assistant Professor**, Lombardi Cancer Center, Georgetown
University, Washington, DC

2000 - 2001 **Postdoctoral Fellow**, Lombardi Cancer Center, Georgetown
University,

> Washington, DC

1998 - 1999 **Postdoctoral Fellow**, Section on Molecular Biology of
Selenium, Basic Research Laboratory, NCI, Bethesda.

1992 - 1995 **Research Assistant**, Dept. of Biochemistry & Molecular
Biology, Hunan Medical University, China.

1987 - 1992 **Pediatrician and Surgeon**, Children\'s Hospital of Hunan
Province, China.

[Publications:]{.underline}

1.  **Xue-Ming Xu**, Bradley A. Carlson, Heiko Mix, Yan Zhang, Kazima
    Saira, Richard S. Glass, Marla J. Berry, Vadim N. Gladyshev and
    Dolph L. Hatfield (2007) Biosynthesis of Selenocysteine on its tRNA
    in Eukaryotes. *PLoS Biology*. *5(1)* *In press*.

2.  Hatfield, D. L., Carlson, B. A., **Xu, X. M.**, Mix, H., and
    Gladyshev, V. N. (2006) Selenocysteine incorporation machinery and
    the role of selenoproteins in development and health., *Prog.
    Nucleic Acid Res. Mol. Biol.* *81*, 93-138.

3.  Small-Howard, A., Morozova, N., Stoytcheva, Z., Forry, E. P.,
    Mansell, J. B., Harney, J. W., Carlson, B. A., **Xu, X. M.**,
    Hatfield, D. L., and Berry, M. J. (2006) Supramolecular complexes
    mediate selenocysteine incorporation in vivo, *Mol. Cell Biol.*
    *26*, 2337-2346.

4.  Yoo, M. H., **Xu, X. M.**, Carlson, B. A., Gladyshev, V. N., and
    Hatfield, D. L. (2006) Thioredoxin reductase 1 deficiency reverses
    tumor phenotype and tumorigenicity of lung carcinoma cells, *J.
    Biol. Chem.* *281*, 13005-13008.

5.  **Xu, X. M.**, Mix, H., Carlson, B. A., Grabowski, P. J.,
    Gladyshev, V. N., Berry, M. J., and Hatfield, D. L. (2005) Evidence
    for direct roles of two additional factors, SECp43 and soluble liver
    antigen, in the selenoprotein synthesis machinery, *J. Biol. Chem.*
    *280*, 41568-41575.

6.  Carlson, B.A., **Xu, X.M**., Gladyshev, V.N., and Hatfield,
    D.L. (2005) Um34 in selenocysteine tRNA is required for the
    expression of stress-related selenoproteins in mammals. In Grosjean
    (Ed.): Fine-Tuning of RNA Functions by Modification and Editing,
    *Topics in Current Genetics, 12, 431-438.*

7.  Carlson, B. A., **Xu, X. M**., Gladyshev, V. N., and
    Hatfield, D. L. (2005) Selective rescue of selenoprotein expression
    in mice lacking a highly specialized methyl group in selenocysteine
    tRNA, *J. Biol. Chem.* *280*, 5542-5548.

8.  Chen, J., **Xu, X. M**., Underhill, C. B., Yang, S., Wang, L., Chen,
    Y., Hong, S., Creswell, K., and Zhang, L. (2005) Tachyplesin
    activates the classic complement pathway to kill tumor cells,
    *Cancer Res.* *65*, 4614-4622.

9.  Shrimali, R. K., Lobanov, A. V., **Xu, X. M.,** Rao, M., Carlson, B.
    A., Mahadeo, D. C., Parent, C. A., Gladyshev, V. N., and
    Hatfield, D. L. (2005) Selenocysteine tRNA identification in the
    model organisms Dictyostelium discoideum and Tetrahymena
    thermophila, *Biochem. Biophys. Res. Commun.* *329*, 147-151.

10. Carlson, B. A., **Xu, X. M.**, Kryukov, G. V., Rao, M., Berry, M.
    J., Gladyshev, V. N., and Hatfield, D. L. (2004) Identification and
    characterization of phosphoseryl- tRNA\[Ser\]Sec kinase, *Proc.
    Natl. Acad. Sci. U. S. A* *101*, 12848-12853.

11. Liu, N., **Xu, X. M**., Chen, J., Wang, L., Yang, S., Underhill, C.
    B., and Zhang, L. (2004) Hyaluronan-binding peptide can inhibit
    tumor growth by interacting with Bcl-2, *Int. J. Cancer* *109*,
    49-57.

12. Wang, L., Yu, J., Ni, J., **Xu, X. M**., Wang, J., Ning, H., Pei, X.
    F., Chen, J., Yang, S., Underhill, C. B., Liu, L., Liekens, J.,
    Merregaert, J., and Zhang, L. (2003) Extracellular matrix protein 1
    (ECM1) is over-expressed in malignant epithelial tumors, *Cancer
    Lett.* *200*, 57-67.

13. **Xu, X. M.**, Chen, Y., Chen, J., Yang, S., Gao, F., Underhill, C.
    B., Creswell, K., and Zhang, L. (2003) A peptide with three
    hyaluronan binding motifs inhibits tumor growth and induces
    apoptosis, *Cancer Res.* *63*, 5685-5690.

14. Yang, S., Chen, J., Guo, Z., **Xu, X. M**., Wang, L., Pei, X. F.,
    Yang, J., Underhill, C. B., and Zhang, L. (2003) Triptolide inhibits
    the growth and metastasis of solid tumors, *Mol. Cancer Ther.* *2*,
    65-72.

15. Liu, N., Shao, L., **Xu, X. M**., Chen, J., Song, H., He, Q., Lin,
    Z., Zhang, L., and Underhill, C. B. (2002) Hyaluronan metabolism in
    rat tail skin following blockage of the lymphatic circulation,
    *Lymphology* *35*, 15-22.

16. **Xu, X. M**., Carlson, B. A., Grimm, T. A., Kutza, J., Berry, M.
    J., Arreola, R., Fields, K. H., Shanmugam, I., Jeang, K. T.,
    Oroszlan, S., Combs, G. F., Jr., Marx, P. A., Gladyshev, V. N.,
    Clouse, K. A., and Hatfield, D. L. (2002) Rhesus monkey simian
    immunodeficiency virus infection as a model for assessing the role
    of selenium in AIDS, *J. Acquir. Immune. Defic. Syndr.* *31*,
    453-463.

17. Berry, M. J., Tujebajeva, R. M., Copeland, P. R., **Xu, X. M**.,
    Carlson, B. A., Martin, G. W., III, Low, S. C., Mansell, J. B.,
    Grundner-Culemann, E., Harney, J. W., Driscoll, D. M., and
    Hatfield, D. L. (2001) Selenocysteine incorporation directed from
    the 3\'UTR: characterization of eukaryotic EFsec and mechanistic
    implications, *Biofactors* *14*, 17-24.

18. Chen, Y., **Xu, X. M**., Hong, S., Chen, J., Liu, N., Underhill, C.
    B., Creswell, K., and Zhang, L. (2001) RGD-Tachyplesin inhibits
    tumor growth, *Cancer Res.* *61*, 2434-2438.

19. Liu, N., Gao, F., Han, Z., **Xu, X. M**., Underhill, C. B., and
    Zhang, L. (2001) Hyaluronan synthase 3 overexpression promotes the
    growth of TSU prostate cancer cells, *Cancer Res.* *61*, 5207-5214.

20. Gladyshev, V. N., Martin-Romero, F. J**., Xu, X. M**., Kumaraswamy,
    E., Carlson, B. A., Hatfield, D. L., and Lee, B. J. (2000) Molecular
    biology of selenium and its role in cancer, AIDS and other human
    disease., *Recent Res. Devel. Biochem.* *1*, 145-167.

21. Tujebajeva, R. M., Copeland, P. R**., Xu, X. M**., Carlson, B. A.,
    Harney, J. W., Driscoll, D. M., Hatfield, D. L., and
    Berry, M. J. (2000) Decoding apparatus for eukaryotic selenocysteine
    insertion, *EMBO Rep.* *1*, 158-163.

22. Gladyshev, V. N., Krause, M., **Xu, X. M**., Korotkov, K. V.,
    Kryukov, G. V., Sun, Q. A., Lee, B. J., Wootton, J. C., and
    Hatfield, D. L. (1999) Selenocysteine-containing thioredoxin
    reductase in C. elegans, *Biochem. Biophys. Res. Commun.* *259*,
    244-249.

23. Kumaraswamy, E., **Xu, X. M**., Martin-Romero, F. J., Carlson, B.
    A., and Hatfield, D. L. (1999) Roles of UGA in mammalian protein
    synthesis: Selenocysteine, stop, suppression and reading gaps,
    *Current Topics in Biochemical Research* *1*, 113-123.

24. **Xu, X. M**., Carlson, B. A., Kim, L. K., Lee, B. J., Hatfield, D.
    L., and Diamond, A. M. (1999) Analysis of selenocysteine (Sec)
    tRNA(\[Ser\]Sec) genes in Chinese hamsters, *Gene* *239*, 49-53.

25. **Xu, X. M**., Zhou, X., Carlson, B. A., Kim, L. K., Huh, T. L.,
    Lee, B. J., and Hatfield, D. L. (1999) The zebrafish genome contains
    two distinct selenocysteine tRNA\[Ser\]sec genes, *FEBS Lett.*
    *454*, 16-20.

26. Ikai, A., Xu, **X. M**., and Mitsui, K. (1998) Measurements of
    mechanical parameters of biological structures with atomic force
    microscope, *Scanning Microscopy* *12*, 585-598.

27. **Xu, X. M**. and Ikai, A. (1998) Recovery and amplification of
    plasmid DNA with atomic force microscopy and the polymerase chain
    reaction., *Anal. Chim. Acta* *361*, 1-7.

28. **Xu, X. M**. and Ikai, A. (1998) Retrieval and amplification of
    single-copy genomic DNA from a nanometer region of chromosomes: a
    new and potential application of atomic force microscopy in genomic
    research, *Biochem. Biophys. Res. Commun.* *248*, 744-748.

29. Ikai, A., Mitsui, K., Tokuoka, H., and **Xu, X. M**. (1997)
    Mechanical measurements of a single protein molecule and human
    chromosomes by atomic force microscopy, *Materials Science &
    Engineering C* *4*, 233-240.

30. **Xu, X. M**. and Ikai, A. (1997) Measurement of mechanical
    properties of human chromosomes by using atomic force microscopy,
    *Cellular Engineering* *2*, 75-81.

**Chapters in Books:**

1.  Bradley A.Carlson, **Xue-Ming Xu**, Rajeev Shrimali, Aniruddha
    Sengupta, Min-Hyuk Yoo, Nianxin Zhong, Dolph L.Hatfield, Robert
    Irons, Cindy D.Davis, Byeong Jae Lee, Sergey V.Novoselov, and Vadim
    N.Gladyshev (2006) Mouse models for assessing the role of selenium
    in health and development, in *Selenium\--Its Molecular Biology and
    Role in Human Health* (Dolph L.Hatfield, Marla J.Berry, and Vadim
    N.Gladyshev, Eds.) 2nd ed., pp 333-342, Springer.

2.  Bradley A.Carlson, **Xue-Ming Xu**, Rajeev Shrimali, Aniruddha
    Sengupta, Min-Hyuk Yoo, Robert Irons, Nianxin Zhong, Dolph
    L.Hatfield, Byeong Jae Lee, Alexey V.Lobanov, and Vadim
    N.Gladyshev (2006) Mammalian and other eukaryotic seleocysteine
    tRNAs, in *Selenium\--Its Molecular Biology and Role in Human
    Health* (Dolph L.Hatfield, Marla J.Berry, and Vadim N.Gladyshev,
    Eds.) 2nd ed., pp 73-82, Springer.

3.  Gustavo Salinas, Héctor Romero, **Xue-Ming Xu**, Bradley A.Carlson,
    Dolph L.Hatfield, and Vadim N.Gladyshev (2006) Evolution of
    selenocysteine decoding and the key role of seleno-phosphate
    synthetase in the pathway of selenium utilization, in
    *Selenium\--Its Molecular Biology and Role in Human Health* (Dolph
    L.Hatfield, Marla J.Berry, and Vadim N.Gladyshev, Eds.) 2nd ed., pp
    39-50, Springer.