of one of the best frames gave 371 dead larvae on one side. The odor was pronounced. The case needed confidence ; it was bad indeed. With me, queenlessness presents the worst of all obstacles. No grubs, no physic, no cure! I had stipulated that the stock should have a queen, and so the difficulty was greater than I had anticipated. Early next morning, seeing the utterly disheartened condition of the poor bees, I went to a nucleus, took out a very fine Italian mother, just proved as purely fecundated, and putting her under a dome cage on a card, placed the card over the frames. The bees came up and seemed to see in her a new hope. The cage was lifted, and she was welcomed immediately. I waited three days, till she was regularly laying, giving syrup phenolated in 500; and now, since I could not create bees, added two combs of brood. This step was made necessary by the fact that I required a strong live by the time of the Congress. The bees were now shut up by a division board ; but the combs put behind it, wanting introduction as the bees multiplied, smelt so badly--the weather being hot--that I several times sprayed them with water 200, phenol 1. Now I should compress the bees as much as possible, and spray the removed combs freely with water 50, phenol 1. To return. Every evening the medicated syrup was given by pouring around brood nest ; but only so much as would be likely to be used, the object not being to fill the cells, but to get the food converted into bees. The smell vanished, the bees became active and earnest. The comb with 371 dead larvae on one side was last added, and in six days I could only find five sunken caps in the whole of it. Now and again a grub took the disease, but quickly perfect immunity was the issue. No cell was uncapped, no disheased grub removed, nor the hive touched, except as described. The bees cleaned their floor and their combs ; while, in four weeks and two or three days, every frame became with brood in the brightest and best ; since this, worse cases have succumbed in the same fashion. Abundant corroboration has been given from those who have tried my method, and have succeeded to their own delight, while some have failed, but the testimony is general that bees under phenol become more energetic than are those that need no treatment. The quantities are easily managed. I oz. of phenol crystals (carbolic acid No. 1) will be sufficient for 40 lbs. of syrup, 1/2 oz. for 10 lbs., or 1/2 oz. of liquid carbolic, P. B., for 9 lbs. syrup or rather less than three quarts. The carbonic acid should be added to the syrup when the latter is cool, and equally mixed by careful stirring. Microscopic examinations of _Bacillus avei_ shows clearly that it is subject to variations in the vigor of its growth. Sometimes the rods are longer and stouter than at others ; but its colony form is quite constant, and the spores and the methods of their production are always the same. In blood serum this bacillus grows with even greater vigor than it does in the body of the bee. Variation, which will account for the disease being sometimes especially virulent, is commonly observed in micro-organisms, and forms the very basis of the system of attenuation for inoculation purposes, as practiced by Pasteur and others. Where the queen is diseased, probably no treatment will be efficacious until she has been replaced. This is a problem which seems to forbid solution, since we have no means of determining the condition of a queen until her life has been sacrificed. Those who believe that the replacement of the queen is all that is needed to effect a cure, will soon get evidence of their palpable error. Pricking a needle into a diseased larva and than touching a larva in a healthy hive with it, is, four times out of six, enough to start a vigorous attack. It could not, however, be supposed that the queen is, in such a case, the cause of the malady. Summer is, without doubt the best time for treatment, as then the bees cannot only more easily bear the necessary disturbance and the chilling, but they can be aroused to rapid brood raising, which involves the application of the remedy.