Reaction SMILES: [NH2:1][C:2]1[S:3][CH:4]=[C:5](/[C:7](=[N:34]/[O:35][CH:36]([C:48]([OH:50])=[O:49])SC2C=CC(OC(=O)C)=CC=2)/[C:8]([NH:10][C@@H:11]2[C:32](=[O:33])[N:13]3[C:14]([C:29]([O-:31])=[O:30])=[C:15]([CH2:18][S:19][C:20]4[S:24][N:23]=[C:22]([OH:25])[C:21]=4[C:26]([OH:28])=[O:27])[CH2:16][S:17][C@H:12]23)=[O:9])[N:6]=1.[Na+:51].[Na+].[Na+].[NH2:54][C:55]1[S:56][CH:57]=[C:58](/[C:60](=[N:87]/[O:88][CH:89](SC2C=CC(OC(=O)C)=CC=2)[C:90]([OH:92])=[O:91])/[C:61]([NH:63][C@@H:64]2[C:85](=[O:86])[N:66]3[C:67]([C:82]([O-:84])=[O:83])=[C:68]([CH2:71][S:72][C:73]4[S:77][N:76]=[C:75]([OH:78])[C:74]=4[C:79]([OH:81])=[O:80])[CH2:69][S:70][C@H:65]23)=[O:62])[N:59]=1.[NH2:104][C:105]1[S:106][CH:107]=[C:108](/[C:110](=[N:137]/[O:138][CH:139](SC2C=CC(OC(=O)C)=CC=2)[C:140]([OH:142])=[O:141])/[C:111]([NH:113][C@@H:114]2[C:135](=[O:136])[N:116]3[C:117]([C:132]([O-:134])=[O:133])=[C:118]([CH2:121][S:122][C:123]4[S:127][N:126]=[C:125]([OH:128])[C:124]=4[C:129]([OH:131])=[O:130])[CH2:119][S:120][C@H:115]23)=[O:112])[N:109]=1.FC(F)(F)C(O)=O>O>[NH2:1][C:2]1[S:3][CH:4]=[C:5](/[C:7](=[N:34]/[O:35][CH:36]([C:48]([OH:50])=[O:49])[C:64]2[CH:85]=[N:66][CH:67]=[CH:68][CH:71]=2)/[C:8]([NH:10][C@@H:11]2[C:32](=[O:33])[N:13]3[C:14]([C:29]([O-:31])=[O:30])=[C:15]([CH2:18][S:19][C:20]4[S:24][N:23]=[C:22]([OH:25])[C:21]=4[C:26]([OH:28])=[O:27])[CH2:16][S:17][C@H:12]23)=[O:9])[N:6]=1.[Na+:51].[Na+:51].[Na+:51].[NH2:54][C:55]1[S:56][CH:57]=[C:58](/[C:60](=[N:87]/[O:88][CH:89]([C:114]2[CH:135]=[N:116][CH:117]=[CH:118][CH:121]=2)[C:90]([OH:92])=[O:91])/[C:61]([NH:63][C@@H:64]2[C:85](=[O:86])[N:66]3[C:67]([C:82]([O-:84])=[O:83])=[C:68]([CH2:71][S:72][C:73]4[S:77][N:76]=[C:75]([OH:78])[C:74]=4[C:79]([OH:81])=[O:80])[CH2:69][S:70][C@H:65]23)=[O:62])[N:59]=1.[NH2:104][C:105]1[S:106][CH:107]=[C:108](/[C:110](=[N:137]/[O:138][CH:139]([C:11]2[CH:32]=[N:13][CH:14]=[CH:15][CH:18]=2)[C:140]([OH:142])=[O:141])/[C:111]([NH:113][C@@H:114]2[C:135](=[O:136])[N:116]3[C:117]([C:132]([O-:134])=[O:133])=[C:118]([CH2:121][S:122][C:123]4[S:127][N:126]=[C:125]([OH:128])[C:124]=4[C:129]([OH:131])=[O:130])[CH2:119][S:120][C@H:115]23)=[O:112])[N:109]=1
Reactant: CC(=O)Oc1ccc(SC(O/N=C(\C(=O)N[C@@H]2C(=O)N3C(C(=O)[O-])=C(CSc4snc(O)c4C(=O)O)CS[C@H]23)c2csc(N)n2)C(=O)O)cc1
Agent: [Na+]
Solvent: O=C(O)C(F)(F)F
Solvent: O
Product: Nc1nc(/C(=N/OC(C(=O)O)c2cccnc2)C(=O)N[C@@H]2C(=O)N3C(C(=O)[O-])=C(CSc4snc(O)c4C(=O)O)CS[C@H]23)cs1
Temperature: 25 °C
Yield: 138 %

Procedure:
After 0.22 g of the compound obtained in Example 6 (7) was added to 25 ml of trifluoroacetic acid, the mixture was stirred at room temperature for an hour. The reaction mixture was cooled to 5° C. and 6 ml of water was added thereto. After stirring at 5° C., the temperature was reverted to room temperature followed by stirring for 2 hours. The solvent was distilled off under reduced pressure and ether was added to the residue to make powders. To the powders was added 100 ml of water and then 0.20 g of sodium hydrogencarbonate. The mixture was adsorbed to DIAION HP20 followed by elution with water-methanol. The fractions containing the desired product were collected. After concentration, the concentrate was freeze dried to give 90 mg (52.9%) of trisodium 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(carboxy)(3-pyridyl)methoxy]imino]acetamido]-3-[[(4-carboxy-3-hydroxy-5-isothiazolyl)thio]methyl]-3-cephem-4-carboxylate.