Reaction SMILES: [NH:1](C(OC(C)(C)C)=O)[C@H:2]([C:10]([NH:12][C@H:13]([C:15]([NH:17][C@H:18]([C:27]([NH:29][C@H:30]([C:33]([N:35]1[CH2:174][CH2:173][CH2:172][C@H:36]1[C:37]([NH:39][C@H:40]([C:62]([NH:64][C@H:65]([C:89]([N:91]1[CH2:171][CH2:170][CH2:169][C@H:92]1[C:93]([NH:95][C@H:96]([C:122]([NH:124][C@H:125]([C:138]([NH:140][CH2:141][C:142]([N:144]1[CH2:168][CH2:167][CH2:166][C@H:145]1[C:146]([NH:148][C@H:149]([C:151]([NH:153][C@H:154]([C:162]([O:164]C)=[O:163])[C@@H:155]([CH3:161])[O:156]C(C)(C)C)=[O:152])[CH3:150])=[O:147])=[O:143])=[O:139])[CH2:126][CH2:127][CH2:128][CH2:129][NH:130]C(OC(C)(C)C)=O)=[O:123])[CH2:97][C:98]1[N:102]=[CH:101][N:100](C(C2C=CC=CC=2)(C2C=CC=CC=2)C2C=CC=CC=2)[CH:99]=1)=[O:94])=[O:90])[CH2:66][C:67](=[O:88])[NH:68]C(C1C=CC=CC=1)(C1C=CC=CC=1)C1C=CC=CC=1)=[O:63])[CH2:41][CH2:42][CH2:43][NH:44][C:45](=[NH:61])[NH:46]S(C1C(C)=C(C)C(OC)=CC=1C)(=O)=O)=[O:38])=[O:34])[CH2:31][SH:32])=[O:28])[CH2:19][C:20](=[O:26])[O:21]C(C)(C)C)=[O:16])[CH3:14])=[O:11])[C@@H:3]([CH3:9])[O:4]C(C)(C)C.[OH-].[Na+]>CO.O1CCOCC1>[NH2:1][C@H:2]([C:10]([NH:12][C@H:13]([C:15]([NH:17][C@H:18]([C:27]([NH:29][C@H:30]([C:33]([N:35]1[CH2:174][CH2:173][CH2:172][C@H:36]1[C:37]([NH:39][C@H:40]([C:62]([NH:64][C@H:65]([C:89]([N:91]1[CH2:171][CH2:170][CH2:169][C@H:92]1[C:93]([NH:95][C@H:96]([C:122]([NH:124][C@H:125]([C:138]([NH:140][CH2:141][C:142]([N:144]1[CH2:168][CH2:167][CH2:166][C@H:145]1[C:146]([NH:148][C@H:149]([C:151]([NH:153][C@H:154]([C:162]([OH:164])=[O:163])[C@@H:155]([CH3:161])[OH:156])=[O:152])[CH3:150])=[O:147])=[O:143])=[O:139])[CH2:126][CH2:127][CH2:128][CH2:129][NH2:130])=[O:123])[CH2:97][C:98]1[N:102]=[CH:101][NH:100][CH:99]=1)=[O:94])=[O:90])[CH2:66][C:67](=[O:88])[NH2:68])=[O:63])[CH2:41][CH2:42][CH2:43][NH:44][C:45](=[NH:46])[NH2:61])=[O:38])=[O:34])[CH2:31][SH:32])=[O:28])[CH2:19][C:20](=[O:21])[OH:26])=[O:16])[CH3:14])=[O:11])[C@@H:3]([CH3:9])[OH:4]
Reactant: COC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@H](CCCCNC(=O)OC(C)(C)C)NC(=O)[C@H](Cc1cn(C(c2ccccc2)(c2ccccc2)c2ccccc2)cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(=O)NC(c1ccccc1)(c1ccccc1)c1ccccc1)NC(=O)[C@H](CCCNC(=N)NS(=O)(=O)c1c(C)cc(OC)c(C)c1C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CS)NC(=O)[C@H](CC(=O)OC(C)(C)C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)OC(C)(C)C)[C@@H](C)OC(C)(C)C)[C@@H](C)OC(C)(C)C
Agent: [Na+]
Agent: [OH-]
Solvent: C1COCCO1
Solvent: CO
Product: C[C@H](NC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)O)[C@@H](C)O
Reaction time: 4 h

Procedure:
0.3 g of BOC-Thr(But)-Ala-Asp(OBut)-Cys-Pro-Arg(Mtr)-Asn(Trt)-Pro-His(Trt)-Lys(Boc)-Gly-Pro-Ala-Thr(But)-OMe (SEQ ID NO:111) is dissolved in 30 ml of methanol; 1.5 ml of 2N NaOH solution are added and the mixture is stirred at 250 for 4 hours. After removal of the solvent, the residue is taken up in water, the pH is adjusted to 3 by adding diluted HCI, and the product is extracted with ethyl acetate. The extract is dried over Na2 SO4. After removal of the solvent, BOC-Thr(But)-Ala-Asp(OBut)-Cys-Pro-Arg(Mtr)-Asn(Trt)-Pro-His(Trt)-Lys(Boc)-Gly-Pro-Ala-Thr(But)-OH (SEQ ID NO:112) is obtained, which is taken up in 20 ml of 2N Hcl in dioxane and stirred at room temperature for 2 hours. The reaction mixture is concentrated to dryness and the residue is taken up in TFA/CH2Cl2, precipitated with Et2O and purified by RP-H PLC, to give H-Thr-Ala-Asp-Cys-Pro-Arg-Asn-Pro-His-Lys-Gly-Pro-Ala-Thr-OH (SEQ ID NO:113); Rt=12.6; M+ =1465.