get away with anything. After 16 weeks they start to learn that they have to follow certain 'rules' in life and gradually they are taught to calm down. Some pups have a 'mad' period just before they crash out to sleep - it is what they would do when they are with the rest of the litter (play hard and sleep hard!). Firstly forget about her trying to become Alpha bitch - dogs really don't think like that, she is not plotting to take over leadership of the household. Sounds to me like she is trying to get you to play and is getting over excited/frustrated when she is not getting a reaction. When you say she becomes quite aggressive what do you mean? Is she trying to nip you? I would either turn my back on her and totally ignore her, or completely walk out of the room and only return when she has become quiet. The 'game' would end immediately. Pups usually have a 'puppy license' up until the age of around 16 weeks, this is where adult dogs will let them get away with anything. After 16 weeks they start to learn that they have to follow certain 'rules' in life and gradually they are taught to calm down. Some pups have a 'mad' period just before they crash out to sleep - it is what they would do when they are with the rest of the litter (play hard and sleep hard!). Thank you all so