If we can't create separate pools in IIS 5.1. Then how to solve this issue on IIS 5.1? I am baffled..Can you please help me how to solve this issue. This is very urgent one ..I will definitely appreciate all your helps. Thanks in advance Faisal. N deploy mutliple applications iis ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^\\... Don't forget to click "Mark as Answer" on the post that helped you. Re: Error ..Could not load the assembly 'App_Web_4giibxae'. Make sure that it is compiled before... I am really sorry for the delay since I didn't check this forum for last few months. The simple solution which I can give you is...Please publish your website by right clicking on the Website in the Solution explorer. Then copy all the files from directory where your published location. Copy those files to your root directory (Do not copy the website folder itself..only files from the directory) and see if it solve your problem. The second solution is .. Please create one virtual directory and point this Virtual directory to your application directory. Hope this solve your issue. Please get back to me if the issue persists ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^\\... Don't forget to click "Mark as Answer" on the post that helped you. Re: Error ..Could not load the assembly 'App_Web_4giibxae'. Make sure that it is compiled before... I am also having same problem, I build a web application in asp2.0 and publish it, then I made a virtual directory on server, then i upload all files and bin folder too.