curation The accuracy of the classification generated by the pipeline was manually verified for all the categories, except "contig", for which the annotation information of genomic regions was not available. This process was performed using a web interface tool described in the alignments procedures section of the methods, where the data on the alignments are reported and graphically displayed (Figure (Figure5, 5 , [ 38 ]). The web interface tool provides a direct external link to the Ensembl website, to allow all the information for a given gene to be easily accessed. Criteria used to evaluate the significance of the alignments, and therefore the correctness of the classification, were: the conservation of gene structure between human and cow, the parameters of alignments and the presence of genscan predictions, ESTs, mRNAs and UniGene features, aligned alongside with the query sequence. Only protein coding genes aligned to the genome with overall identity equal or greater than 75% were selected for the manual curation, even though the sequences below this threshold had highly significant E-values. The genes in the "not aligned" category, which could not be selected using a threshold, were all examined. The analyses and supporting evidence for each library is presented in the following sections and the results summarised in Table Table3 3 . Web interface to display the results of alignments of the bovine and human libraries . Records can be retrieved by Ensembl ID, percentage of identity of the alignment, classification and gene name, through the search panel. For