off, if you want to quantify the fluorescent. Have a look at the two images to the right for the difference between smooth brightness turned on and off. Controls to adjust the background of the selected images. Controls to adjust the brightness of the selected images Button to toggle the visibility of the channel. Determines if the adjustments are applied to all images within the selection or just to the image with the focus. Resets the adjustment values back to the original values. Automatically adjusts the background and the brightness of the selected images. Applies the current adjustment values to the rest of the selected images.