Dataset Article "Species composition shapes canopy microclimate and foliar traits without modifying photosynthetic thermal acclimation in a beech-oak ecotone", Deluigi et al.

**Article: "Species Species composition shapes canopy microclimate and foliar traits without modifying photosynthetic thermal acclimation in a beech-oak ecotone"**
Authors: **Janisse Deluigi**, Charlotte Grossiord, Alvaro Poretti, Lorenz Walthert, Christoph Bachofen
The study was conducted along a south-exposed elevational gradient ranging from 770 to 860 m a.s.l. in a central alpine valley (46°10`14``N, 7°10`01``E, Saillon, Switzerland). Along the elevational gradient, we selected three forest stands characterized by high coverage of mature European beech (Fagus sylvatica L.) and downy oak (Quercus pubescens Willd.) trees, with are naturally distributed in three species combinations: one pure beech stand (i.e., at least 80% of the basal area) at 860 m a.s.l. , one mixed beech-oak stand (i.e., similar relative abundances of the two species – at least 40% of the basal area each – and minimal presence of other tree species) at 785 m a.s.l., and one pure oak stand (i.e., at least 80% of the basal area) at 770 m a.s.l. An additional forest clearing of around 0.04 ha next to the pure oak stand was selected as reference for conditions outside the forest  (i.e., clearing or gap). At each forest stand, we selected five healthy, mature, overstory trees per tree species, resulting in a total of 20 individuals (5 individuals x 2 species x 2 mixture combinations, i.e., mixed vs. pure forest stand). 
**Dataset 1: Climatic conditions at the study sites**
In 2023 and 2024 we monitored climatic conditions at each forest stand and in the clearing using climatic stations with air temperature, relative humidity and radiation sensors installed at 1.8 m height in a central position relative to the selected trees for each site. The meteo station in the clearing site had additionally a rain gauge and wind sensor. 
**Dataset 2: Tree and canopy sizes**
Once in 2023 we measured tree height and diameter at breast height of all selected trees using a vertex hypsometer and a caliper. In August 2023 and 2024 we estimated canopy size measuring the height at which canopy starts (CS), canopy depth (= tree height - CS) and maximum canopy radius as the average of two orthogonal measurements (south and east direction) using a vertex hypsometer. We also estimated canopy total leaf area using species- and site-specific allometric equations from collected branches upscaled to the tree level.
**Dataset 3: Microclimatic conditions measured in tree canopies**
We monitored canopy microclimatic conditions in 2023 and 2024 attaching one air temperature and air humidity sensor (HOBO) on one branch at the top and one branch at the bottom of the south-exposed side of the canopy for each selected tree in June 2023.
**Dataset 4: Physiological traits**
During three field campaigns (June, August, September) per year (2023, 2024) we measured temperature response curves of leaf net photosynthesis and dark respiration. As in-situ measurements were not possible, tree-climbers collected one representative branch at the top and the bottom of the canopy from each selected tree at each site. Estimates of the thermal optimum of photosynthesis (Topt), photosynthetic rate at Topt (Aopt), respiration rate at a standard temperature of 25°C (R25) and thermal sensitivity of respiration (Q10) were extracted from the temperature response curves of photosynthesis and respiration, respectively. 
Leaf water potentials at predawn and midday, as well as leaf chlorophyll content, were also measured on the same day for each selected tree at each site. 
**Dataset 5: Physiological traits - specific leaf area and leaf size**
In August 2023 and 2024 we collected a random subset of 10 representative fully developed leaves of different sizes from a representative branch harvested at the top and bottom of the canopy of each selected tree to estimate the specific leaf area (SLA) and leaf size (average of the 10 leaves).
**Dataset 6: Soil water potentials**
Hourly soil water potentials and soil temperature were recorded during the two years of measurements (2023, 2024) at each forest site at different soil depths: 20 cm, 80 cm, 140 cm and 200 cm.

Tags: acclimation, climate-change, forest-canopy, photosynthesis, respiration, species-interaction