AndreaBozzo/ceres-open-data-index huggingface dataset:
Phorbol ester impairs electrical excitation of rat pancreatic beta-cells through PKC-independent activation of K

Background
          Phorbol 12-myristate 13-acetate (PMA) is often used as an activating phorbol ester of protein kinase C (PKC) to investigate the roles of the kinase in cellular functions. Accumulating lines of evidence indicate that in addition to activating PKC, PMA also produces some regulatory effects in a PKC-independent manner. In this study, we investigated the non-PKC effects of PMA on electrical excitability of rat pancreatic β-cells by using patch-clamp techniques.
        
        
          Results
          In current-clamp recording, PMA (80 nM) reversibly inhibited 15 mM glucose-induced action potential spikes superimposed on a slow membrane depolarization and this inhibition can not be prevented by pre-treatment of the cell with a specific PKC inhibitor, bisindolylmaleimide (BIM, 1 μM). In the presence of a subthreshold concentration (5.5 mM) of glucose, PMA hyperpolarized β-cells in a concentration-dependent manner (0.8–240 nM), even in the presence of BIM. Based on cell-attached single channel recordings, PMA increased ATP-sensitive K+ channel (KATP) activity. Based on inside-out patch-clamp recordings, PMA had little effect on KATP activity if no ATP was in the bath, while PMA restored KATP activity that was suppressed by 10 μM ATP in the bath. In voltage-clamp recording, PMA enhanced tolbutamide-sensitive membrane currents elicited by repetitive ramp pulses from -90 to -50 mV in a concentration-dependent manner, and this potentiation could not be prevented by pre-treatment of cell with BIM. 4α-phorbol 12,13-didecanoate (4α-PDD), a non-PKC-activating phorbol ester, mimicked the effect of PMA on both current-clamp and voltage-clamp recording configurations. With either 5.5 or 16.6 mM glucose in the extracellular solution, PMA (80 nM) increased insulin secretion from rat islets. However, in islets pretreated with BIM (1 μM), PMA did not increase, but rather reduced insulin secretion.
        
        
          Conclusion
          In rat pancreatic β-cells, PMA modulates insulin secretion through a mixed mechanism: increases insulin secretion by activation of PKC, and meanwhile decrease insulin secretion by impairing β-cell excitability in a PKC-independent manner. The enhancement of KATP activity by reducing sensitivity of KATP to ATP seems to underlie the PMA-induced impairment of β-cells electrical excitation in response to glucose stimulation.

Tags: electrical-excitability, nih, pancreatic-beta-cells, patch-clamp, phorbol-ester
Organization: U.S. Department of Health & Human Services
2018-2019 National Survey on Drug Use and Health National Maps of Prevalence Estimates, by State

The 116 national maps presented here show the 2018-2019 National Survey on Drug Use and Health (NSDUH) estimates for 30 substance use and mental health outcomes, by age group, for 50 states and the District of Columbia.

Tags: mental-health, nsduh-data, samhsa, state-prevalence, substance-use
Organization: U.S. Department of Health & Human Services
Compound 525241: N''-(3-methoxy-phenyl)-N,N,N',N'-tetramethyl-guanidine

Chemical compound data from PubChem database. IUPAC Name: 2-(3-methoxyphenyl)-1,1,3,3-tetramethylguanidine. Molecular Formula: C12H19N3O. Molecular Weight: 221.3. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Osmotic stress-dependent serine phosphorylation of the histidine kinase homologue DokA

Background
          Two-component systems consisting of histidine kinases and their corresponding receivers are widespread in bacterial signal transduction. In the past few years, genes coding for homologues of two-component systems were also discovered in eukaryotic organisms. DokA, a homologue of bacterial histidine kinases, is an element of the osmoregulatory pathway in the amoeba Dictyostelium. The work described here addresses the question whether DokA is phosphorylated in vivo in response to osmotic stress.
        
        
          Results
          We have endogenously overexpressed individual domains of DokA to investigate post-translational modification of the protein in response to osmotic shock in vivo. Dictyostelium cells were labeled with [32P]-orthophosphate, exposed to osmotic stress and DokA fragments were subsequently isolated by immunoprecipitation. Thus, a stress-dependent phosphorylation could be demonstrated, with the site of phosphorylation being located in the kinase domain. We demonstrate biochemically that the phosphorylated amino acid is serine, and by mutational analysis that the phosphorylation reaction is not due to an autophosphorylation of DokA. Furthermore, mutation of the conserved histidine did not affect the osmostress-dependent phosphorylation reaction.
        
        
          Conclusions
          A stimulus-dependent serine phosphorylation of a eukaryotic histidine kinase homologue was demonstrated for the first time in vivo. That implies that DokA, although showing typical structural features of a bacterial two-component system, might be part of a eukaryotic signal transduction pathway that involves serine/threonine kinases.

Tags: histidine-kinase, nih, osmotic-stress, protein-phosphorylation, signal-transduction
Organization: U.S. Department of Health & Human Services
Compound 525081: 1-(10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethane-1,2-diol

Chemical compound data from PubChem database. IUPAC Name: 1-(10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)ethane-1,2-diol. Molecular Formula: C21H36O2. Molecular Weight: 320.5. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
2018 Uniform Reporting System (URS) Table for New Hampshire

This is the 2018 Uniform Reporting System (URS) Output Tables for New Hampshire. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-program, mental-health-services, national-outcome-measures, samhsa, urs-data
Organization: U.S. Department of Health & Human Services
The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it

Background
          Experimental colitis with features similar to inflammatory bowel disease (IBD) has initially been described. A detailed analysis of inflammatory cells has not yet been described. Therefore in this study we characterized the cells involved in the acute phase of the colitis and compared those findings to what is known about human IBD.
        
        
          Methods
          Colitis was induced in BALB/C and C57Bl6 mice by ingestion of 2.5% and 5% DSS in the drinking water for 8 days. Cells were labelled by immunohistochemical staining with F4/80 and ER-MP20 for macrophages, TIB 120 for MHC Class II presentation, and anti-CD4 and anti-CD8 antibodies. They were enumerated by using a novel method that employs video image analysis. Immunoglobulin-producing cells were enumerated by immunofluorescent staining for IgA, IgG and IgM and counting by using confocal microscopy.
        
        
          Results
          Inflammatory infiltrate in the acute phase of the dextran sulphate sodium (DSS) -induced colitis consists predominantly of macrophages, neutrophils and eosinophils. Neutrophils increase in numbers and crypt abscesses were also seen. Increased macrophage numbers were due to recently recruited monocytes from the peripheral circulation. It does not appear that there are any changes in T cell numbers or distribution. The inflammation induced changes in immunoglobulin-producing cells with IgA-producing cells affected the most.
        
        
          Conclusions
          The effect on Ig-producing cells depends on the percentage of DSS used to induce colitis. In general, 2.5% DSS induces an increase and 5% DSS a depletion of these cells.

Tags: dss-colitis, immunohistochemistry, inflammatory-infiltrate, mouse-model, nih
Organization: U.S. Department of Health & Human Services
Pharmacological and ischemic preconditioning of the human myocardium: mitoK

Background
          These studies investigate the role of mitoKATP channels, protein kinase C (PKC) and Mitogen activated protein kinase (p38MAPK) on the cardioprotection of ischemic (IP) and pharmacological preconditioning (PP) of the human myocardium and their sequence of activation.
        
        
          Results
          Right atrial appendages from patients undergoing elective cardiac surgery were equilibrated for 30 min and then subjected to 90 min of simulated ischemia followed by 120 min reoxygenation. At the end of each protocol creatinine kinase leakage (CK U/g wet wt) and the reduction of MTT to formazan dye (mM/g wet wt) were measured. Similar protection was obtained with α1 agonist phenylephrine, adenosine and IP and their combination did not afford additional cardioprotection. Blockade of mitoKATP channels with 5-hydroxydecanoate, PKC with chelerythrine, or p38MAPK with SB203580 abolished the protection of IP and of PP. In additional studies, the stimulation of mitoKATP channels with diazoxide or activation of PKC with PMA or p38MAPK with anisomycin induced identical protection to that of IP and PP. The protection induced by diazoxide was abolished by blockade of PKC and by blockade of p38MAPK. Furthermore, the protection induced by PMA was abolished by SB203580 but not by 5-hydroxydecanoate, whereas the protection induced by anisomycin was unaffected by either 5-hydroxydecanoate or chelerythrine.
        
        
          Conclusions
          Opening of mitoKATP channels and activation of PKC and p38MAPK are obligatory steps in the signal transduction cascade of IP and PP of the human myocardium with PKC activation being downstream of the opening of mitoKATP channels and upstream of p38MAPK activation.

Tags: creatine-kinase, mitok-atp-channels, myocardial-ischemia, nih, preconditioning-cardioprotection
Organization: U.S. Department of Health & Human Services
P-ANCA vasculitic neuropathy with 12-year latency between onset of neuropathy and systemic symptoms

Background
          The differential diagnosis of chronic progressive multifocal asymmetric neuropathies is challenging. Vasculitic neuropathies, multifocal forms of chronic inflammatory demyelinating polyneuropathy, multifocal motor neuropathies, and asymmetric lower motor neuron disorders are important considerations.
        
        
          Case presentation
          We report a patient with an unusually long 12-year course of nonsystemic vasculitic neuropathy prior to the development of systemic manifestations.
        
        
          Conclusion
          We discuss some of the difficulties involved in the diagnosis of chronic progressive multifocal asymmetric neuropathies.

Tags: multifocal-neuropathy, neuropathy-latency, nih, p-anca-vasculitis, vasculitic-neuropathy
Organization: U.S. Department of Health & Human Services
Adapting a Promising Multi-Faceted Child Maltreatment Preventive Intervention to Respond to Differences in Target Populations

A presentation on the use of Washoe County's  SAFE-Family Connections intervention with families with children determined to be unsafe, part of the Nevada Initiative to Reduce Long-Term Foster Care / Permanency Innovations Initiative (PII).

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: foster, intervention, maltreatment, policy, prevention
Organization: U.S. Department of Health & Human Services
2021 Uniform Reporting System (URS) Table For Kansas

This is the 2021 Uniform Reporting System (URS) Output Tables for Kansas. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant, kansas, mental-health-services, outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
Guide to Drug Abuse Warning Network Trend Tables, 2010

The guide to tne DAWN Excel files that contain the 2004-2010 annual estimates, with definitions of the \"analytic groups\" (topics) covered by the files; descriptions of the content and major features of the tables, and infomation about the statistics provided in the tables.

Tags: dawn-data, drug-abuse-trends, drug-related-hospitalizations, samhsa, substance-abuse-statistics
Organization: U.S. Department of Health & Human Services
Receipt of Services for Behavioral Health Problems: Results from the 2014 National Survey on Drug Use and Health

This report is the third (in a series of four) regarding the First Release Reports for the 2014 NSDUH.  This report presents data from the 2002 through 2014 NSDUHs for substance use treatment and mental health service use among people aged 12 or older. Results are presented for adolescents aged 12 to 17 and for adults aged 18 or older. Where trends are presented, the report focuses on long-term trends by comparingestimates from the 2014 NSDUH with comparable estimates in 2002 to 2013 (or in 2008 to 2013 for some estimates of mental health service use). Statistically significant differences are noted between estimates in 2014 and those in prior years.

Tags: behavioral-health, mental-health-services, nsduh-survey, samhsa, substance-use-treatment
Organization: U.S. Department of Health & Human Services
2022 Uniform Reporting System (URS) Table For New Mexico

This is the 2022 Uniform Reporting System (URS) Output Tables for New Mexico. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-data, mental-health-services, new-mexico, noms-measures, samhsa
Organization: U.S. Department of Health & Human Services
Compound 527209: 2-(8,8a-Dimethyl-1,3,4,6,7,8-hexahydronaphthalen-2-ylidene)propan-1-ol

Chemical compound data from PubChem database. IUPAC Name: 2-(8,8a-dimethyl-1,3,4,6,7,8-hexahydronaphthalen-2-ylidene)propan-1-ol. Molecular Formula: C15H24O. Molecular Weight: 220.35. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Compound 525042: 3',4'-Dichloroenanthoanilide

Chemical compound data from PubChem database. IUPAC Name: N-(3,4-dichlorophenyl)heptanamide. Molecular Formula: C13H17Cl2NO. Molecular Weight: 274.18. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Manipulation of the follicular phase: Uterodomes and pregnancy - is there a correlation?

Background
          Manipulation of the follicular phase uterine epithelium in women undergoing infertility treatment, has not generally shown differing morphological effects on uterine epithelial characteristics using Scanning Electron Microscopy (SEM) and resultant pregnancy rates have remained suboptimal utilising these manipulations. The present study observed manipulation of the proliferative epithelium, with either 7 or 14 days of sequential oestrogen (E) therapy followed by progesterone (P) and assessed the appearance of pinopods (now called uterodomes) for their usefulness as potential implantation markers in seven women who subsequently became pregnant. Three endometrial biopsies per patient were taken during consecutive cycles: day 19 of a natural cycle - (group 1), days 11/12 of a second cycle after 7 days E then P - (group 2), and days 19/22 of a third cycle after 14 days E then P - (group 3). Embryo transfer (ET) was performed in a subsequent long treatment cycle (as per Group 3).
        
        
          Results
          Seven pregnancies resulted in seven viable births including one twins and one miscarriage. Analysis of the individual regimes showed 5 days of P treatment to have a higher correlation for uterodomes in all 3 cycles observed individually. It was also observed that all 7 women demonstrated the appearance of uterodomes in at least one of their cycles.
        
        
          Conclusions
          We conclude that manipulation of the follicular phase by shortening the period of E exposure to 7 days, does not compromise uterine epithelial morphology and we add weight to the conclusion that uterodomes indicate a receptive endometrium for implantation.

Tags: endometrial-biopsy, follicular-phase, infertility-treatment, nih, uterodomes
Organization: U.S. Department of Health & Human Services
Compound 525567: N,N-Dimethyl-N'-(3-chlorophenyl)-p-methoxybenzamidine

Chemical compound data from PubChem database. IUPAC Name: N'-(3-chlorophenyl)-4-methoxy-N,N-dimethylbenzenecarboximidamide. Molecular Formula: C16H17ClN2O. Molecular Weight: 288.77. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Perturbations in the impact of mutational activity on Vλ genes in systemic lupus erythematosus

To assess the impact of somatic hypermutation and selective influences on the Vλ light chain repertoire in systemic lupus erythematosus (SLE), the frequency and pattern of mutations were analyzed in individual CD19+ B cells from a patient with previously undiagnosed SLE. The mutational frequency of nonproductive and productive rearrangements in the SLE patient was greater (3.1 × 10-2 vs 3.4 × 10-2, respectively) than that in normal B cells (1.2 × 10-2 vs 2.0 × 10-2, both P < 0.001). The frequencies of mutated rearrangements in both the nonproductive and productive repertoires were significantly higher in the patient with SLE than in normal subjects. Notably, there were no differences in the ratio of replacement to silent (R/S) mutations in the productive and nonproductive repertoires of the SLE patient, whereas the R/S ratio in the framework regions of productive rearrangements of normal subjects was reduced, consistent with active elimination of replacement mutations in this region. The pattern of mutations was abnormal in the SLE patient, with a significant increase in the frequency of G mutations in both the productive and nonproductive repertoires. As in normal subjects, however, mutations were found frequently in specific nucleotide motifs, the RGYW/WRCY sequences, accounting for 34% (nonproductive) and 46% (productive) of all mutations. These data are most consistent with the conclusion that in this SLE patient, the mutational activity was markedly greater than in normal subjects and exhibited some abnormal features. In addition, there was decreased subsequent positive or negative selection of mutations. The enhanced and abnormal mutational activity along with disturbances in selection may play a role in the emergence of autoreactivity in this patient with SLE.

Tags: b-cell-repertoire, nih, somatic-hypermutation, systemic-lupus-erythematosus, v-lambda-genes
Organization: U.S. Department of Health & Human Services
PRESTA: associating promoter sequences with information on gene expression

The software tool PRESTA (PRomoter EST Association) is designed for efficient recovery of characterized and partially verified promoters from GenBank and EMBL libraries. The PRESTA algorithm demonstrates the principle of promoter verification by mapping EST 5' ends.

Tags: bioinformatics-tool, est-mapping, gene-expression, nih, promoter-sequences
Organization: U.S. Department of Health & Human Services
Compound 525107: 2,3-Dichloropropyl heptanoate

Chemical compound data from PubChem database. IUPAC Name: 2,3-dichloropropyl heptanoate. Molecular Formula: C10H18Cl2O2. Molecular Weight: 241.15. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
National Directory of Drug and Alcohol Abuse Treatment Facilities - 1984

The National Directory of Drug and Alcohol Abuse Treatment Programs 1984 lists federal, state, local, and private facilities that provide substance abuse treatment services. The directory includes treatment facilities that are licensed, certified, or otherwise approved for inclusion in the directory by their state substance abuse agencies and that responded to the 1984 National Survey of Substance Abuse Treatment Services (N-SSATS). The information about each facility that appears in this directory was provided by that facility in response to the 1983 N-SSATS. The Substance Abuse and Mental Health Services Administration (SAMHSA) conducts the N-SSATS annually.

Tags: alcohol-abuse-treatment, n-ssats, samhsa, substance-abuse-treatment, treatment-facilities
Organization: U.S. Department of Health & Human Services
Baltimore-Towson, MD

This report presents combined 2005 to 2010 National Survey on Drug Use and Health (NSDUH) results for those aged 12 or older in the Baltimore-Towson, Maryland metro area. The report includes estimates of substance use disorder, major depressive episode, illicit drug use, marijuana use, cigarette use, binge alcohol use, and the nonmedical use of prescription-type pain relievers. Results are provided for Baltimore-Towson, Maryland, and the U.S. overall.

Tags: baltimore-towson, major-depression, nsduh-data, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Clinical review: Use of vancomycin in haemodialysis patients

Following intravenous administration, vancomycin is poorly metabolized and is mainly excreted unchanged in urine. Total body clearance is thus dependent on the kidney, and is correlated with glomerular filtration rate and creatinine clearance. Accumulation of vancomycin in patients with renal insufficiency may therefore occur, and this may lead to toxic side effects if dosage is not modified according to the degree of renal failure. Furthermore, vancomycin easily diffuses through dialysis membranes. The aim of the present review is to establish guidelines for handling this drug in such patients. We indicate how and when plasma concentrations of vancomycin should be determined in dialysis patients.

Tags: drug-dosage, hemodialysis-patients, nih, renal-insufficiency, vancomycin-pharmacokinetics
Organization: U.S. Department of Health & Human Services