Data from: Topical application of synthetic hormones terminated reproductive diapause to facilitate rearing of a univoltine weevil for weed biological control agent

<p>These are results of a series of laboratory experiments to determine if topical application of methoprene and 20-ecdysone can terminate reproductive diapause of the weevil, <em>Ceratapion basicorne</em>, which is a recently permitted biological control agent of yellow starthistle (<em>Centaurea solstitialis</em>).  Adult weevils feed on leaves, creating pin holes, and lay eggs inside leaves.  Diapausing weevils were treated with various doses of methoprene (0, 0.01, 0.1, 1.0 micrograms) dissolved in acetone in experiments 1 and 2. They were treated sequentially first with acetone or 20-ecdysone (1.0 microgram) and then with methoprene (1.0 microgram) in experiment 3 and were treated with 20-ecdysone followed by methoprene in experiment 4. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: data dictionary.</p> <p>File Name: JH Data Dictionary.csv</p><p>Resource Description: description of data fields</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/microsoft-365/excel">https://www.microsoft.com/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: experiment 1.</p> <p>File Name: JH expt1 data.csv</p><p>Resource Description: Methoprene dissolved in acetone was applied topically at doses of 0.0, 0.01 and 0.1 and 1.0 μg per female weevil, and the number of feeding holes and eggs were recorded daily on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21°C).</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/microsoft-365/excel">https://www.microsoft.com/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: experiment 2.</p> <p>File Name: JH expt2 data.csv</p><p>Resource Description: Methoprene dissolved in acetone was applied topically at doses of 0.0 and 1.0 μg to female weevils that did not produce eggs in experiment 1.  The number of feeding holes and eggs were recorded daily on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21°C).</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/microsoft-365/excel">https://www.microsoft.com/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: experiment 3.</p> <p>File Name: JH expt3 data.csv</p><p>Resource Description: Three types of treatments were applied with sequential applications 2 days apart: 1) acetone + acetone [AA: control], 2) acetone + methoprene [AM], and 20-ecdysone + methoprene 174 [2M].  All doses were 1.0 μg.  The number of feeding holes and eggs were recorded every 2 days on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21°C).</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/microsoft-365/excel">https://www.microsoft.com/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: experiment 4.</p> <p>File Name: JH expt4 data.csv</p><p>Resource Description: Females from experiment 3 that did not oviposit consistently were treated with 1.0 μg of 20-ecdysone followed 2 days later by 1.0 μg of methoprene.  The treatments AA, AM, 2M refer to experiment 3.  The number of feeding holes and eggs were recorded every 2 days on cut leaves of yellow starthistle at room temperature (12 h photoperiod, temperature range 17 to 21°C).</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/microsoft-365/excel">https://www.microsoft.com/microsoft-365/excel</a> </p></li></ul><p></p>

Tags: ars, data-gov, ecdysone, juvenile-hormone, methoprene, np304, reproductive-diapause, weevil
Organization: Department of Agriculture