AndreaBozzo/ceres-open-data-index huggingface dataset:
Methylation and silencing of the retinoic acid receptor-β2 gene in cervical cancer

Background
          Expression of the retinoic acid receptor β2 (RAR-β2), a putative tumor suppressor gene, is reduced in various human cancers, including squamous cell carcinomas (SCC) of the uterine cervix. The mechanism of the inhibition of RAR-β2 expression remains obscure. We examined whether methylation of RAR-β2 gene could be responsible for this silencing in cervical SCC.
        
        
          Methods
          Expression of RAR-β2 mRNA and methylation status of the 5' region of RAR-β2 gene were examined in 20 matched specimens from patients with cervical SCC and in three cervical cancer cell lines by Northern blot analysis and methylation-specific PCR (MSP) assay or Southern blot analysis respectively.
        
        
          Results
          In 8 out 20 cervical SCC (40%) the levels of RAR-β2 mRNA were decreased or undetectable in comparison with non-neoplastic cervix tissues. All 8 tumors with reduced levels of RAR-β2 mRNA expression showed methylation of the promoter and the first exon expressed in the RAR-β2 transcript. The RAR-β2 gene from non-neoplastic cervical tissues was mostly unmethylated and expressed, but methylated alleles of the gene were found in three samples of the morphologically normal tissues adjacent to the tumors. Three cervical cancer cell lines with extremely low level of RAR-β2 mRNA expression, SiHA, HeLA and CaSki, also showed methylation of this region of the RAR-β2 gene.
        
        
          Conclusions
          These findings suggest that methylation of the 5' region of RAR-β2 gene may contribute to gene silencing and that methylation of this region may be an important and early event in cervical carcinogenesis. These findings may be useful to make retinoids more effective as preventive and therapeutic agents in combination with inhibitors of DNA methylation.

Tags: cervical-cancer, gene-methylation, nih, rar-beta2, tumor-suppressor-genes
Organization: U.S. Department of Health & Human Services
National Directory of Drug and Alcohol Abuse Treatment Facilities 2012

The National Directory of Drug and Alcohol Abuse Treatment Programs 2012 lists federal, state, local, and private facilities that provide substance abuse treatment services. The directory includes treatment facilities that are licensed, certified, or otherwise approved for inclusion in the directory by their state substance abuse agencies and that responded to the 2011 National Survey of Substance Abuse Treatment Services (N-SSATS). The information about each facility that appears in this directory was provided by that facility in response to the 2011 N-SSATS. The Substance Abuse and Mental Health Services Administration (SAMHSA) conducts the N-SSATS annually.

Tags: alcohol-abuse, drug-abuse, samhsa, substance-abuse-treatment, treatment-facilities
Organization: U.S. Department of Health & Human Services
Compound 525630: N,N-Dimethyl-N'-hexyl-benzamidine

Chemical compound data from PubChem database. IUPAC Name: N'-hexyl-N,N-dimethylbenzenecarboximidamide. Molecular Formula: C15H24N2. Molecular Weight: 232.36. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
24-hour efficacy of once-daily desloratadine therapy in patients with seasonal allergic rhinitis [ISRCTN32042139]

Background
          Early studies with desloratadine demonstrated efficacy in treating seasonal allergic rhinitis (SAR). A dose-ranging study was conducted to characterize its 24-hour efficacy in patients with SAR.
        
        
          Methods
          Patients (N = 1,026) were randomly assigned once-daily (QD) desloratadine (2.5, 5, 7.5, 10, or 20 mg) for 2 weeks in a placebo-controlled, double-blind study. The end point of 24-hour efficacy was assessed by the mean change from baseline in the average AM instantaneous total symptom score (TSS) over the treatment period. Day 2 data were assessed for efficacy of desloratadine following the first dose. Other efficacy variables included AM/PM previous total nasal and nonnasal symptom scores and individual symptom scores.
        
        
          Results
          Desloratadine 5–20 mg was significantly (P < .01) more effective than placebo in improving total AM instantaneous TSS and AM/PM previous total nasal and nonnasal symptom scores. This dosing range also was significantly (P < .01) more effective than placebo for reducing AM instantaneous TSS beginning with the first dose; thus, demonstrating the full 24-hour efficacy of desloratadine. AM/PM previous scores for all individual symptoms, including nasal congestion, were also significantly improved versus placebo (P < .05) with desloratadine at 5, 7.5, and 20 mg. All treatments were well tolerated. There were no clinically meaningful changes in electrocardiogram parameters.
        
        
          Conclusion
          Desloratadine 5–20 mg provided significant 24-hour relief of SAR signs and symptoms. There were no statistically significant differences between the 4 largest doses suggesting that desloratadine 5 mg QD offers the best therapeutic profile for patients with SAR.

Tags: allergy-symptoms, clinical-trial, desloratadine-therapy, nih, seasonal-allergic-rhinitis
Organization: U.S. Department of Health & Human Services
Clinical review: Hypertonic saline resuscitation in sepsis

The present review discusses the hemodynamic effects of hypertonic saline in experimental shock and in patients with sepsis. We comment on the mechanisms of action of hypertonic saline, calling upon data in hemorrhagic and septic shock. Specific actions of hypertonic saline in severe sepsis and septic shock are highlighted. Data are available that support potential benefits of hypertonic saline infusion in various aspects of the pathophysiology of sepsis, including tissue hypoperfusion, decreased oxygen consumption, endothelial dysfunction, cardiac depression, and the presence of a broad array of proinflammatory cytokines and various oxidant species. The goal of research in this field is to identify reliable therapies to prevent ischemia and inflammation, and to reduce mortality.

Tags: hemodynamic-effects, hypertonic-saline, nih, sepsis-treatment, septic-shock
Organization: U.S. Department of Health & Human Services
Polyethyleneimine-mediated transfection of cultured postmitotic neurons from rat sympathetic ganglia and adult human retina

Background
          Chemical methods of transfection that have proven successful with cell lines often do not work with primary cultures of neurons. Recent data, however, suggest that linear polymers of the cation polyethyleneimine (PEI) can facilitate the uptake of nucleic acids by neurons. Consequently, we examined the ability of a commercial PEI preparation to allow the introduction of foreign genes into postmitotic mammalian neurons. Sympathetic neurons were obtained from perinatal rat pups and maintained for 5 days in vitro in the absence of nonneuronal cells. Cultures were then transfected with varying amounts of a plasmid encoding either E. coli β-galactosidase or enhanced green fluorescence protein (EGFP) using PEI.
        
        
          Results
          Optimal transfection efficiency was observed with 1 μg/ml of plasmid DNA and 5 μg/ml PEI. Expression of β-galactosidase was both rapid and stable, beginning within 6 hours and lasting for at least 21 days. A maximum yield was obtained within 72 hours with ∼ 9% of the neurons expressing β-galactosidase, as assessed by both histochemistry and antibody staining. Cotransfection of two plasmids encoding reporter genes was achieved. Postmitotic neurons from adult human retinal cultures also demonstrated an ability to take up and express foreign DNA using PEI as a vector.
        
        
          Conclusions
          These data suggest that PEI is a useful agent for the stable expression of plasmid-encoded genes in neuronal cultures.

Tags: gene-expression, neuron-transfection, nih, polyethyleneimine, retinal-neurons
Organization: U.S. Department of Health & Human Services
Compound 528464: 1-Tetradecene, 8-ethyl

Chemical compound data from PubChem database. IUPAC Name: 8-ethyltetradec-1-ene. Molecular Formula: C16H32. Molecular Weight: 224.42. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Compound 525388: 2,6,6-Trimethyl-5-thiaoctane

Chemical compound data from PubChem database. IUPAC Name: 2-methyl-2-(3-methylbutylsulfanyl)butane. Molecular Formula: C10H22S. Molecular Weight: 174.35. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
2022 Uniform Reporting System (URS) Table For Minnesota

This is the 2022 Uniform Reporting System (URS) Output Tables for Minnesota. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, evidence-based-practices, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
2019 Uniform Reporting System (URS) Table for Pennsylvania

These are the 2019 Uniform Reporting System (URS) Output Tables for Pennsylvania. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, evidence-based-practices, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
2010-2011 NSDUH Guide to State Tables and Summary of Small Area Estimation Methodology

This report presents the methodology for calculating State estimates for measures of substance use and mental health based on the combined 2009 and 2010 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE).

Tags: mental-health, nsduh-data, samhsa, small-area-estimation, substance-use
Organization: U.S. Department of Health & Human Services
Effects on respiratory function of the head-down position and the
		  complete covering of the face by drapes during insertion of the monitoring
		  catheters in the cardiosurgical patient

Background:
          We evaluated the effect on the respiratory gas exchange of the
				30° head-down position and the complete covering of the face by sterile
				drapes. These are used to cannulate the internal jugular vein and position the
				pulmonary artery catheter in the cardiosurgical patient. During the two
				manoeuvres, 20 coronary patients and 10 patients with end-stage heart disease
				were supplied with oxygen (FiO2 =0.4) by a
				Venturi mask, while 20 coronary patients breathed room air. The arterial blood
				samples to measure oxygen (PaO2) and carbon dioxide
				(PaCO2) tension and oxygen saturation (SaO2) were
				analysed by a blood gas system.
        
        
          Results:
          The contemporary application of the head-down position and the
				drapes over the face significantly increased PaO2 and
				SaO2 in all the patientssupplied with oxygen. Without the head-down
				position, leaving the drapes over the face, did not significantly change the
				two parameters in the coronary patients supplied with oxygen, but induced a
				significant increase in PaO2 and SaO2 in the patients
				with end-stage heart disease. In the coronary patients that were breathing room
				air, PaO2 and SaO2 were stable throughout the study.
        
        
          Conclusions:
          We conclude that the 30° head-down position and the complete
				covering of the face by drapes does not interfere with respiratory gas exchange
				and can be safely performed in coronary patients supplied with oxygen or
				breathing room air and in patients with end-stage heart disease supplied with
				oxygen (FiO2 of 0.4).

Tags: blood-gas-analysis, cardiac-surgery, head-down-position, nih, respiratory-function
Organization: U.S. Department of Health & Human Services
2017 Uniform Reporting System (URS) Table for Kentucky

This is the 2017 Uniform Reporting System (URS) Output Tables for Kentucky. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-data, kentucky-urs, mental-health-services, noms-measures, samhsa
Organization: U.S. Department of Health & Human Services
2017 NSDUH Detailed Tables

Results from the 2017 National Survey on Drug Use and Health: Detailed Tables is a collection of tables presenting national estimates from the National Survey on Drug Use and Health (NSDUH). These tables present information for youths aged 12 to 17 and adults aged 18 or older (separately and combined) on drug, alcohol, and tobacco use, as well as substance use disorder (SUD), risk and availability of substance use, treatment, health topics, and alcohol consumption. For youths, additional topics include youth experiences and measures on mental health service utilization, major depressive episode (MDE), and treatment for depression (among youths with MDE). For adults, additional topics include measures on any mental illness (AMI), serious mental illness (SMI), AMI excluding SMI, mental health service utilization (i.e., treatment or counseling for mental health issues), suicidal thoughts and behaviors, MDE, treatment for depression (among adults with MDE), and serious psychological distress (SPD). Measures such as the co-occurrence of mental disorders with substance use or with SUDs also are presented for both adults and youths. Measures of these behaviors and characteristics are presented by a variety of demographic, geographic, and other variables. The estimates in the tables include prevalence rates of the behaviors, numbers of persons engaging in these behaviors, and other measures.

Tags: alcohol-tobacco, mental-health, samhsa, substance-use, suicidal-ideation
Organization: U.S. Department of Health & Human Services
Table 25. Had Serious Thoughts of Suicide in the Past Year, by Age Group and State: Percentages, Annual Averages Based on 2013 and 2014 NSDUHs

Table 25. Had Serious Thoughts of Suicide in the Past Year, by Age Group and State: Percentages, Annual Averages Based on 2013 and 2014 NSDUHs

Tags: age-groups, mental-health, samhsa, state-level-data, suicidal-ideation
Organization: U.S. Department of Health & Human Services
Compound 525608: N,N-Dimethyl-N'-(4-nitrophenyl)-p-methoxybenzamidine

Chemical compound data from PubChem database. IUPAC Name: 4-methoxy-N,N-dimethyl-N'-(4-nitrophenyl)benzenecarboximidamide. Molecular Formula: C16H17N3O3. Molecular Weight: 299.32. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
2010 CMHS Uniform Reporting System (URS) Output Tables for Nebraska

Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for 2010. The Uniform Reporting System (URS) is a state and national reporting system collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, evidence-based-practices, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
Nevada: 2011-2012 NSDUH

This report presents Nevada\'s estimates for 25 measures of substance use and mental health based on the combined 2011 and 2012 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Resuts are provided by State and for five age groups: 12 to 17, 18 to 25, 26 or older, 18 or older and all persons 12 or older.

Tags: mental-health, nevada-nsduh, samhsa, serious-mental-illness, substance-use
Organization: U.S. Department of Health & Human Services
Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo – a new animal model

Background
          Existing animal models provide only indirect information about the pathogenesis of infections caused by indigenous gastrointestinal microflora and the kinetics of bacterial translocation. The aim of this study was to develop a novel animal model to assess bacterial translocation and intestinal barrier function in vivo.
        
        
          Methods
          In anaesthetized male Wistar rats, 0.5 ml of a suspension of green fluorescent protein-transfected E. coli was administered by intraluminal injection in a model of small bowel obstruction. Animals were randomly subjected to non-ischemic or ischemic bowel obstruction. Ischemia was induced by selective clamping of the terminal mesenteric vessels feeding the obstructed bowel loop. Time intervals necessary for translocation of E. coli into the submucosal stroma and the muscularis propria was assessed using intravital microscopy.
        
        
          Results
          Bacterial translocation into the submucosa and muscularis propria took a mean of 36 ± 8 min and 80 ± 10 min, respectively, in small bowel obstruction. Intestinal ischemia significantly accelerated bacterial translocation into the submucosa (11 ± 5 min, p < 0.0001) and muscularis (66 ± 7 min; p = 0.004). Green fluorescent protein-transfected E. coli were visible in frozen sections of small bowel, mesentery, liver and spleen taken two hours after E. coli administration.
        
        
          Conclusions
          Intravital microscopy of fluorescent bacteria is a novel approach to study bacterial translocation in vivo. We have applied this technique to define minimal bacterial transit time as a functional parameter of intestinal barrier function.

Tags: bacterial-translocation, bowel-obstruction, intravital-microscopy, ischemia-reperfusion, nih
Organization: U.S. Department of Health & Human Services
Transjugular renal biopsy in high-risk patients: an American case series

Background
          In the United States, transjugular renal biopsies using the Quickcore™ side cut needle system have previously been described primarily for transjugular renal biopsy in patients with concurrent liver and kidney disease.
        
        
          Methods
          We describe transjugular renal biopsy with the Quickcore™ system in 9 patients with nephrotic syndrome and contraindications to percutaneous renal biopsy, who underwent biopsy between 23 October 1996 and 12 April 2001. The most common contraindication was oral anticoagulation with coumadin (40%). Other contraindications included horseshoe kidney, severe renal failure, and spontaneous coagulopathy. A 62 cm straight catheter and 60 cm side-cut Quickcore™ biopsy needle were used to obtain cortical tissue. Packing of the biopsy tract with Gelfoam™ was used for venographically identified capsular perforation.
        
        
          Results
          Ten procedures were performed on 9 patients with one requiring re-biopsy (5% of all renal biopsies performed at our institution). There were 9 transjugular renal biopsy and one combined liver-kidney biopsy. A mean of 4 ± 2 passes were made, with a mean of 3 ± 1 cores obtained per procedure. Histologic diagnosis was made in 90% of biopsies and in 100% of patients. Two patients developed transient hydronephrosis associated with gross hematuria; both required transfusion. Capsular perforation occurred in 90%. One patient died of bacterial sepsis, unrelated to the biopsy, several days after the procedure.
        
        
          Conclusions
          Transjugular renal biopsy appears to be efficacious in high-risk patients, for whom the percutaneous approach is contraindicated, including patients on oral anticoagulation. The transfusion rate in the present study was similar to other American reports using this technique.

Tags: high-risk-patients, kidney-disease, nih, renal-biopsy, transjugular-approach
Organization: U.S. Department of Health & Human Services
South: 2011-2012 NSDUH

This report presents the South\'s (e.g., combines Alabama, Arkansas, Delaware, District of Columbia, Florida, Georgia, Kentucky, Louisiana, Maryland, Mississippi, North Carolina, Oklahoma, South Carolina, Tennessee, Texas, Virginia, and West Virginia) estimates for 25 measures of substance use and mental health based on the combined 2011 and 2012 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Resuts are provided for the South for five age groups: 12 to 17, 18 to 25, 26 or older, 18 or older and all persons 12 or older.

Tags: mental-health, nsduh, samhsa, south-region, substance-use
Organization: U.S. Department of Health & Human Services