AndreaBozzo/ceres-open-data-index huggingface dataset:
Stress responses and replication of plasmids in bacterial cells

Plasmids, DNA (or rarely RNA) molecules which replicate in cells autonomously (independently of chromosomes) as non-essential genetic elements, play important roles for microbes grown under specific environmental conditions as well as in scientific laboratories and in biotechnology. For example, bacterial plasmids are excellent models in studies on regulation of DNA replication, and their derivatives are the most commonly used vectors in genetic engineering. Detailed mechanisms of replication initiation, which is the crucial process for efficient maintenance of plasmids in cells, have been elucidated for several plasmids. However, to understand plasmid biology, it is necessary to understand regulation of plasmid DNA replication in response to different environmental conditions in which host cells exist. Knowledge of such regulatory processes is also very important for those who use plasmids as expression vectors to produce large amounts of recombinant proteins. Variable conditions in large-scale fermentations must influence replication of plasmid DNA in cells, thus affecting the efficiency of recombinant gene expression significantly. Contrary to extensively investigated biochemistry of plasmid replication, molecular mechanisms of regulation of plasmid DNA replication in response to various environmental stress conditions are relatively poorly understood. There are, however, recently published studies that add significant data to our knowledge on relations between cellular stress responses and control of plasmid DNA replication. In this review we focus on plasmids derived from bacteriophage λ that are among the best investigated replicons. Nevertheless, recent results of studies on other plasmids are also discussed shortly.

Tags: bacterial-stress-response, dna-replication, genetic-engineering, nih, plasmid-replication
Organization: U.S. Department of Health & Human Services
2021 Uniform Reporting System (URS) Table For Washington

This is the 2021 Uniform Reporting System (URS) Output Tables for Washington. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, evidence-based-practices, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
Alabama (AL)

This report presents Alabama\'s estimates for 25 measures of substance use and mental health based on the combined 2009 and 2010 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Resuts are provided for Alabama for five age groups: 12 to 17, 18 to 25, 26 or older, 18 or older and all persons 12 or older.

Tags: alabama-health, mental-health, nsduh-data, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
2, 4-Diamino-6- hydroxy pyrimidine inhibits NSAIDs induced nitrosyl-complex EPR signals and ulcer in rat jejunum

Background
          It has been suggested that one aspect of non-steroidal anti-inflammatory drugs induced intestinal damage is due to either uncoupling of mitochondrial oxidative phosphorylation or inhibition of electron transport. We investigated the latter possibility using electron paramagnetic resonance spectroscopy.
        
        
          Results
          Electron paramagnetic studies of NSAIDS on sub-mitochondrial particles revealed that indomethacin, but not with nabumetone, bound to a site near to Complex I and ubiquinone to generate a radical species. Normal rats exhibited prominent [3Fe-4S]ox signals (g ~ 2.01) at 20 K. One hour after indomethacin there was a prominent, intense and broad absorption pattern at (g ~2.07) suggesting, appearance of radical species overlapping [3Fe-4S]ox and was unaffected by pretreatment with 2,4 diamino -6-hydroxy pyrimidine. At 24 hrs, when macroscopic ulcers were seen, there was a new signal due to a nitric oxide radical (NO•). In contrast, nabumetone and 2,4 diamino-6-hydroxy pyrimidine pre-treated animals receiving indomethacin exhibited electron paramagnetic resonance spectra identical to those of controls at 24 hrs and neither was associated with small intestinal ulcers. Indomethacin and 2,4 diamino hydroxy pyrimidine pre-treated rats, but not nabumetone, had increased intestinal permeability.
        
        
          Conclusion
          The results suggest that the in vivo effects of indomethacin modulate the mitochondrial respiratory chain directly at 1 h and 24 h through formation of nitric oxide. NO• appears to play an important role in the late pathogenic stages of NSAID enteropathy and may be the site for targeted treatment to reduce their toxicity.

Tags: electron-paramagnetic-resonance, indomethacin, nih, nitric-oxide-radical, nsaid-induced-ulcer
Organization: U.S. Department of Health & Human Services
4.16b Facility licensing, certification, or accreditation, by type of care offered: 2010. Percent

This is a National Survey of Substance Abuse Treatment Services (N-SSATS) data table showing facility licensing, certification, or accreditation, by type of care offered in 2010. N-SSATS is designed to collect data on the location, characteristics, and use of alcohol and drug abuse treatment facilities and services throughout the 50 States, the District of Columbia, and other U.S. jurisdictions.

Tags: addiction-services, facility-licensing, n-ssats, samhsa, substance-abuse-treatment
Organization: U.S. Department of Health & Human Services
Automated Child Welfare Information System Options for Tribal Title IV-E Agencies

This Information Memorandum (IM) provides introductory information to title IV-E tribes that choose to develop and implement an automated Child Welfare Information System. This IM contains information about how an automated system can support a tribal title IV-E agency’s child welfare program, implementing and maintaining systems, title IV-E funding, and the Comprehensive Child Welfare Information System (CCWIS).

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: child, guidance, policy, system, tribal
Organization: U.S. Department of Health & Human Services
2015 NSDUH Field Statistical Experience Report

The goal of this report is to further document the 2015 NSDUH sample experiences, including a comparison of actual sample yields to state and quarter targets, a comparison of achieved and expected design effects (DEFFs) and relative standard errors (RSEs), and documentation of any issues encountered during sample implementation.

Tags: nsduh, samhsa, sample-design, statistical-analysis, survey-methodology
Organization: U.S. Department of Health & Human Services
Abundant protein domains occur in proportion to proteome size

Background
          Conserved domains in proteins have crucial roles in protein interactions, DNA binding, enzyme activity and other important cellular processes. It will be of interest to determine the proportions of genes containing such domains in the proteomes of different eukaryotes.
        
        
          Results
          The average proportion of conserved domains in each of five eukaryote genomes was calculated. In pairwise genome comparisons, the ratio of genes containing a given conserved domain in the two genomes on average reflected the ratio of the predicted total gene numbers of the two genomes. These ratios have been verified using a repository of databases and one of its subdivisions of conserved domains.
        
        
          Conclusions
          Many conserved domains occur as a constant proportion of proteome size across the five sequenced eukaryotic genomes. This raises the possibility that this proportion is maintained because of functional constraints on interacting domains. The universality of the ratio in the five eukaryotic genomes attests to its potential importance.

Tags: eukaryotic-genomes, genome-comparison, nih, protein-domains, proteome-size
Organization: U.S. Department of Health & Human Services
Behavioral Health Barometer: Iowa, 2014

This is a behavioral health report for the state of Iowa. It uses the National Survey on Drug Use and Health (NSDUH) and the National Survey of Substance Abuse Treatment Services (NSSATS) to address the topics of youth substance use, youth mental health and treatment, adult mental health and treatment, substance use, and substance use treatment for the years 2009 to 2013.

Tags: behavioral-health, iowa-health, mental-health, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Compound 528531: Hexadecane, 6-octyl

Chemical compound data from PubChem database. IUPAC Name: 9-pentylnonadecane. Molecular Formula: C24H50. Molecular Weight: 338.7. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
NSDUH 2021 Editing And Imputation Report

Learn about the editing and statistical imputation procedures that were applied to respondent data for the 2021 National Survey on Drug Use and Health (NSDUH). Logical editing resolves inconsistencies or ambiguous data based on a respondent’s answers to other questions in the survey. Statistical imputation uses mathematical techniques to assign values when they are missing in the data.Introductory Chapters:An introduction, including a discussion of changes from the 2020 to 2021 survey.A description of the procedures and general principles for editing the NSDUH data.A description of the general imputation procedures used in NSDUH.Remaining chapters are descriptions of the editing and imputation for the following types of variables:Front-end demographics.Back-end demographics.Substance use.Special drugs and substance use disorder.Additional substance use, including treatment and emerging issues.Substance use risk and protective factors.Physical and mental health.Roster variables.Income.Health insurance.Pair variables.

Tags: data-editing, data-imputation, mental-health, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Compound 528385: Benzaldehyde, 3,4,6-trichloro-2-hydroxy

Chemical compound data from PubChem database. IUPAC Name: 3,4,6-trichloro-2-hydroxybenzaldehyde. Molecular Formula: C7H3Cl3O2. Molecular Weight: 225.5. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Emergency Department Visits Involving Marijuana among Adolescents Aged 15 to 17: Increase from 2005 to 2010 Varied by Gender

This Drug Abuse Warning Network (DAWN) spotlight is about emergency department visits involving marijuana among adolescents aged 15 to 17 increase from 2005 to 2010 varied by gender.

Tags: adolescent-marijuana-use, emergency-department-visits, gender-differences, samhsa, substance-abuse
Organization: U.S. Department of Health & Human Services
Compound 525125: 1,3-Benzenedicarboxylic acid, diheptyl ester

Chemical compound data from PubChem database. IUPAC Name: diheptyl benzene-1,3-dicarboxylate. Molecular Formula: C22H34O4. Molecular Weight: 362.5. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Compound 525689: p-Chlorobenzylidene-hexyl-amine

Chemical compound data from PubChem database. IUPAC Name: 1-(4-chlorophenyl)-N-hexylmethanimine. Molecular Formula: C13H18ClN. Molecular Weight: 223.74. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation

Background
          Cytomegalovirus (CMV) pneumonitis has been shown to be associated with lymphocytic alveolitis after lung transplantation. In the present study, we investigated a series of bronchoalveolar (BAL) and blood samples, collected in the absence of rejection or acute infectious episodes. in order -1: to evaluate intra-alveolar cell population changes concomitant with CMV replication and -2: to reappraise the value of cell population analysis in the management of patients after lung transplantation.
        
        
          Methods
          We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. After exclusion of samples obtained during pulmonary rejection, bronchiolitis obliterans or acute bacterial infection, 48 blood and BAL samples were retained for analysis: 17 were CMV positive by shell-vial assay and 31 were CMV negative in blood and BAL.
        
        
          Results
          Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples.
        
        
          Conclusions
          Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection.

Tags: bronchoalveolar-lavage, cytomegalovirus-pneumonitis, lung-transplantation, lymphocyte-subsets, nih
Organization: U.S. Department of Health & Human Services
Profound effect of normalization on detection of differentially expressed genes in oligonucleotide microarray data analysis

A number of procedures for normalization and detection of differentially expressed genes have been proposed. Four different normalization methods and all possible combinations with three different statistical algorithms have been used for detection of differentially expressed genes on a dataset. The number of genes detected as differentially expressed differs by a factor of about three.

Tags: differential-expression, gene-expression, microarray-data, nih, normalization-methods
Organization: U.S. Department of Health & Human Services
Compound 527204: 2-(4a,8-dimethyl-4,5,6,7,8,8a-hexahydro-3H-naphthalen-2-yl)propanal

Chemical compound data from PubChem database. IUPAC Name: 2-(4a,8-dimethyl-4,5,6,7,8,8a-hexahydro-3H-naphthalen-2-yl)propanal. Molecular Formula: C15H24O. Molecular Weight: 220.35. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
The effect of massage on localized lumbar muscle fatigue

Background
          There is not enough evidence to support the efficacy of massage for muscle fatigue despite wide utilization of the modality in various clinical settings. This study investigated the influence of massage application on localized back muscle fatigue.
        
        
          Methods
          Twenty-nine healthy subjects participated in two experimental sessions (massage and rest conditions). On each test day, subjects were asked to lie in the prone position on a treatment table and perform sustained back extension for 90 seconds. Subjects then either received massage on the lumbar region or rested for a 5 minute duration, then repeated the back extension movement. The median frequency (MDF), mean power frequency (MNF), and root mean square (RMS) amplitude of electromyographic signals during the 90 second sustained lumbar muscle contraction were analyzed. The subjective feeling of fatigue was then evaluated using the Visual Analogue Scale (VAS).
        
        
          Results
          MDF and MNF significantly declined with time under all conditions. There was no significant difference in MDF, MNF or RMS value change between before and after massage, or between rest and massage conditions. There was a significant increase in fatigue VAS at the end of the 2nd back extension with rest condition. There was a significant difference in fatigue VAS change between massage and rest condition.
        
        
          Conclusions
          A significant difference was observed between massage and rest condition on VAS for muscle fatigue. On EMG analysis, there were no significant differences to conclude that massage stimulation influenced the myoelectrical muscle fatigue, which is associated with metabolic and electrical changes.

Tags: electromyography, lumbar-muscle, massage-therapy, muscle-fatigue, nih
Organization: U.S. Department of Health & Human Services
Encouraging good antimicrobial prescribing practice: A review of antibiotic prescribing policies used in the South East Region of England

Background
          Good prescribing practice has an important part to play in the fight against antimicrobial resistance. Whilst it was perceived that most hospitals and Health Authorities possessed an antibiotic policy, a review of antibiotic policies was conducted to gain an understanding of the extent, quality and usefulness of these policies.
        
        
          Methods
          Letters were sent to pharmacists in hospitals and health authorities in across the South East region of the National Health Service Executive (NHSE) requesting antibiotic policies. data were extracted from the policies to assess four areas; antibiotic specific, condition specific, patient specific issues and underpinning evidence.
        
        
          Results
          Of a possible 41 hospital trusts and 14 health authorities, 33 trusts and 9 health authorities (HAs) provided policies. Both trust and HA policies had a median publication date of 1998 (trust range 1993-99, HA 1994-99). Eleven policies were undated. The majority of policies had no supporting references for the statements made. All policies provided some details on specific antibiotics. Gentamicin and ciprofloxacin were the preferred aminoglycoside and quinolone respectively with cephalosporins being represented by cefuroxime or cefotaxime in trusts and cephradine or cephalexin in HAs. 26 trusts provided advice on surgical prophylaxis, 17 had meningococcal prophylaxis policies and 11 covered methicillin resistant Staphylococcus aureus (MRSA). There was little information for certain groups such as neonates or children, the pregnant or the elderly.
        
        
          Conclusion
          There was considerable variation in content and quality across policies, a clear lack of an evidence base and a need to revise policies in line with current recommendations.

Tags: antibiotic-prescribing, antimicrobial-resistance, england-nhs, nih, prescribing-policy
Organization: U.S. Department of Health & Human Services
The ESAT-6 gene cluster of

Background
          The genome of Mycobacterium tuberculosis H37Rv has five copies of a cluster of genes known as the ESAT-6 loci. These clusters contain members of the CFP-10 (lhp) and ESAT-6 (esat-6) gene families (encoding secreted T-cell antigens that lack detectable secretion signals) as well as genes encoding secreted, cell-wall-associated subtilisin-like serine proteases, putative ABC transporters, ATP-binding proteins and other membrane-associated proteins. These membrane-associated and energy-providing proteins may function to secrete members of the ESAT-6 and CFP-10 protein families, and the proteases may be involved in processing the secreted peptide.
        
        
          Results
          Finished and unfinished genome sequencing data of 98 publicly available microbial genomes has been analyzed for the presence of orthologs of the ESAT-6 loci. The multiple duplicates of the ESAT-6 gene cluster found in the genome of M. tuberculosis H37Rv are also conserved in the genomes of other mycobacteria, for example M. tuberculosis CDC1551, M. tuberculosis 210, M. bovis, M. leprae, M. avium, and the avirulent strain M. smegmatis. Phylogenetic analyses of the resulting sequences have established the duplication order of the gene clusters and demonstrated that the gene cluster known as region 4 (Rv3444c-3450c) is ancestral. Region 4 is also the only region for which an ortholog could be found in the genomes of Corynebacterium diphtheriae and Streptomyces coelicolor.
        
        
          Conclusions
          Comparative genomic analysis revealed that the presence of the ESAT-6 gene cluster is a feature of some high-G+C Gram-positive bacteria. Multiple duplications of this cluster have occurred and are maintained only within the genomes of members of the genus Mycobacterium.

Tags: cfp-10, esat-6-loci, mycobacterium-tuberculosis, nih, t-cell-antigens
Organization: U.S. Department of Health & Human Services
An international survey of patients with thalassemia major and their views about sustaining life-long desferrioxamine use

Background
          Management of thalassemia major requires patients to have life-long access to a treatment regimen of regular blood transfusions coupled with iron chelation therapy. The objective of this study was to investigate patients' reasons for missing iron chelation therapy with desferrioxamine, and the support to sustain life-long adherence to treatment.
        
        
          Methods
          From October 1999 to May 2000 a survey of patients with thalassemia major was conducted in ten countries: Cyprus, Egypt, Greece, Hong Kong, India, Iran, Italy, Jordan, Taiwan, and the United States.
        
        
          Results
          1,888 questionnaires (65%) were returned. Most patients (1,573) used desferrioxamine, and 79% administered a dose at least 4 days a week. Inaccessibility of the drug was a common reason for missing a dose in India (51%), and in Iran (25%), whereas, in any other country, it was a reason for less than 17% of patients. Overall, 58% reported reasons for missing a dose related to their beliefs or feelings about the medication, and 42% drug-related side effects.
        
        
          Conclusion
          Many patients miss doses of desferrioxamine and an opportunity remains to develop interventions that provide more support to sustain use of desferrioxamine.

Tags: desferrioxamine-adherence, iron-chelation-therapy, nih, patient-survey, thalassemia-major
Organization: U.S. Department of Health & Human Services
Behavioral Health Barometer: HHS Region VII, 2014

This is a behavioral health report for HHS Region VII of the United States and utilizes data from the National Survey on Drug Use and Health (NSDUH) and the National Survey of Substance Abuse Treatment (N-SSATS), collected annually. The topics addressed in the reports include youth mental health and treatment, substance use and substance use treatment.

Tags: behavioral-health, hhs-region-vii, mental-health, samhsa, substance-use
Organization: U.S. Department of Health & Human Services