AndreaBozzo/ceres-open-data-index huggingface dataset:
Sentinel-3A OLCI Regional Binned Inland Waters (ILW) Data, version 4

The Inland Waters dataset (ILW) provides data for lakes and other water bodies across the contiguous United States (CONUS) and Alaska. ILW significantly reduces the processing effort required by end users and is a standardized community resource for lake and reservoir algorithm development and performance assessment. The data is provided for 15,450 CONUS waterbodies with a size of at least one 300 m pixel and over 2,300 resolvable lakes with sizes greater than three 300 m pixels. Alaska has 5,874 lakes resolvable lakes. ILW was developed in collaboration with the Cyanobacteria Assessment Network (CyAN). Additional inland water details and resources, including maps of resolvable lakes and additional inland water products, such as true color imagery, are available at the CyAN site.

Tags: aquatic-sciences, bacteria-archaea, biological-classification, biosphere, coastal-processes, earth-science, earth-science-services, ecosystems, environmental-advisories, environmental-governance-management, human-dimensions, hydrological-advisories, marine-environment-monitoring, ocean-optics, oceans, surface-water, terrestrial-hydrosphere, water-quality-water-chemistry
Organization: National Aeronautics and Space Administration
Re-Adaption on Earth after Spaceflights Affects the Mouse Liver Proteome

Harsh environmental conditions including microgravity and radiation during prolonged spaceflights are known to alter hepatic metabolism. Our studies have focused on the analysis of possible changes in metabolic pathways in livers of mice which experienced 30 days of spaceflight with and without an additional re-adaption period of 7 days compared to control mice on Earth. Utilizing shotgun mass spectrometry and label-free quantification we performed proteomic profiling to investigate mice livers from the spaceflight project  xe2 x80 x9cBion-M 1 xe2 x80 x9d. No significant alterations in protein levels were observed between control mice liver and spaceflight mice which is possibly caused by insufficient fold change detection combined with high variances within the groups. In contrast our results show that more than a third of the quantified protein levels are altered comparing the liver proteome of mice with and without re-adaption time after their spaceflight. Proteins related to amino acid metabolism showed higher levels after re-adaption which may indicate higher rates of gluconeogenesis. Members of the peroxisome proliferator-activated receptor pathway reconstitute their level after 7 days due to a decrease in fold change which indicates decreased signs of non-alcoholic fatty liver disease. Moreover bile acid secretion regenerates on Earth due to reconstitution of related transmembrane proteins and elevated levels of the drug-metabolising enzymes belonging to the CYP superfamily decrease 7 days after the spaceflight. Thus our study demonstrates reconstitution of pharmacological response and early signs of non-alcoholic fatty liver disease recover within 7 days whereas glucose uptake should be monitored due to alterations in gluconeogenesis.

Tags: absorbed-radiation-dose, data-transformation, dissection-timeline, extraction, mass-spectrometry, mouse-habitation, mouse-habitation-protocol, radiation-dosimetry, sample-collection, spaceflight
Organization: National Aeronautics and Space Administration
Genechip analysis of bone marrow osteoprogenitors exposed to microgravity

In March 2006 murine Bone Marrow Stromal Cells (BMSC) were flown in the Soyuz 12S to the International Space Station to investigate the effects of microgravity on their osteogenic potential in a three-dimensional environment. BMSC were grown in porous bioceramic Skelite disks (? 9 mm x T 1.2 mm). The constructs were exposed to microgravity for ca. 8 days then fixed for RNA extraction. While the flight experiment was performed in fully automated hardware inside the KUBIK incubator one group of control samples were incubated inside manually operated hardwares (flight control) and the other control group was incubated under routine laboratory conditions (lab control). The altered gene expression profile was analyzed by Mouse Gene 1.0 ST array (Affymetrix) representing whole-transcript coverage. Each one of the 28853 genes is represented on the array by approximately 26 probes spread across the full length of the gene providing a more complete and more accurate picture of gene expression than the 3 xc3 x94 xc3 xb8 xce xa9 based expression array design. A few days of microgravity were sufficient to determinate at least at the molecular level an effect in the BMSC; this response expressed a stress condition able to determinate consequences on several compartments and cellular functions. In particular it seems to promote a gene expression known to be associated with neurogenic activity (e.g. axon guidance) perhaps promoting the BMSC capability to be committed in that direction. The osteo-induction by dexamethasone-based medium due to the short duration of stimulation did not have the possibility to manifest itself at the phenotypic level but only partially at the molecular level.

Tags: bioassay_data_transformation, control-group, feature_extraction, gravity-type, grow, hybridization, image_aquisition, labeling, nucleic_acid_extraction, osteo-induced, p-gse17696-1, p-gse17696-10, p-gse17696-11, p-gse17696-2, p-gse17696-3, p-gse17696-4, p-gse17696-5, p-gse17696-6, p-gse17696-7, p-gse17696-8, p-gse17696-9, specified_biomaterial_action
Organization: National Aeronautics and Space Administration
PSRPI Mapping the Galactic distribution of pulsars with the VLBA

Observations for the initial PSRPI sample of 60 pulsars have now concluded. 84.7 hours were observed on the VLBA (plus 12 hours from a pilot project) searching for in-beam calibrators around 245 pulsars, and 690.2 hours were utilised for 285 astrometric observations.

Tags: agn, fermi, kait
Organization: National Aeronautics and Space Administration
Exposure of zebrafish larvae to 3g and 5g hyper gravity between 5-6 days post-fertilization.

The general objective of the study was to determine modulation of gene expression by environmental factors, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a Large Diameter Centrifuge and brought to a gravitational force of 3g or 5g for 24 hours. We show that this treatment causes a clear increase of bone formation, as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red, by morphometric analysis of the resulting images and by gene expression studies of selected genes. Thus, a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.

Tags: array-scanning-and-feature-extraction-protocol, data-transformation, genelab-microarray-data-processing-protocol, growth-protocol, hypergravity, normalization-data-transformation-protocol, nucleic-acid-extraction-protocol, nucleic-acid-hybridization, nucleic-acid-labeling-protocol, p-mtab-43156, p-mtab-43157, p-mtab-43158, p-mtab-43159, p-mtab-43160, p-mtab-43161, p-mtab-43162, treatment-protocol
Organization: National Aeronautics and Space Administration
A whole-genome microarray study of Arabidopsis thaliana cell cultures exposed to microgravity for 5 days on board of Shenzhou 8

Arabidopsis thaliana wild type cell cultures were exposed to a 5-day space flight onboard of Shenzhou 8 to identify microgravity and space effect related gene expression.

Tags: altered-gravity, data-collection, data-transformation, genelab-microarray-data-processing-protocol, growth-protocol, labeling, nucleic-acid-hybridization, rna-extraction, spaceflight, treatment-protocol
Organization: National Aeronautics and Space Administration
Gene expression data from 4T1 irradiated tumors treated with TGFbeta blockade

Accumulating data support the concept that ionizing radiation therapy (RT) has the potential to convert the tumor into an in situ individualized vaccine; however this potential is rarely realized by RT alone. Transforming growth factor  xce xb2 (TGF xce xb2) is an immunosuppressive cytokine that is activated by RT and inhibits the antigen-presenting function of dendritic cells and the differentiation of effector CD8+ T cells. Here we tested the hypothesis that TGF xce xb2 hinders the ability of RT to promote anti-tumor immunity. Development of tumor-specific immunity was examined in a pre-clinical model of metastatic breast cancer. Mice bearing established 4T1 mouse mammary carcinoma treated with pan-isoform specific TGF xce xb2 neutralizing antibody 1D11 showed significantly improved control of the irradiated tumor and non-irradiated metastases but no effect in the absence of RT. Notably whole tumor transcriptional analysis demonstrated the selective upregulation of genes associated with immune-mediated rejection only in tumors of mice treated with RT+TGF xce xb2 blockade. Mice treated with RT+TGF xce xb2 blockade exhibited cross-priming of CD8+ T cells producing IFN xce xb3 in response to three tumor-specific antigens in tumor-draining lymph nodes which was not evident for single modality treatment. Analysis of the immune infiltrate in mouse tumors showed a significant increase in CD4+ and CD8+ T cells only in mice treated with the combination of RT+TGF xce xb2 blockade. Depletion of CD4+ or CD8+ T cells abrogated the therapeutic benefit of RT+TGF xce xb2 blockade. These data identify TGF xce xb2 as a master inhibitor of the ability of RT to generate an in situ tumor vaccine which supports testing inhibition of TGF xce xb2 during radiotherapy to promote therapeutically effective anti-tumor immunity. We used genome-wide microarray to depict main biological processes responsibles for the therapeutic benefit of the combination ofTGF-beta blockade and local radiotherapy. To gain a more comprehensice protrait of the effects of RT and TGFbeta blockade on gene expressionin tumors we collected 4T1 tumors 4 days after completion of RT. Three tumors from each group were then subjected to RNA extraction and hybridization on affymetrix array.

Tags: array-scanning-protocol, dose, growth-protocol, hybridization-protocol, injection, ionizing-radiation, labelling-protocol, normalization-data-transformation-protocol, nucleic-acid-extraction-protocol, p-gse61208-1, p-gse61208-2, p-gse61208-3, p-gse61208-4, p-gse61208-5, p-gse61208-6, p-gse61208-7, sample-treatment-protocol
Organization: National Aeronautics and Space Administration
Rodent Research-1 (RR1) NASA Validation Flight: Mouse liver transcriptomic proteomic and epigenomic data

RR-1 is a validation flight to evaluate the hardware operational and science capabilities of the Rodent Research Project on the ISS. RNA DNA and protein were purified from liver tissues from RR-1 mice (female C57Bl6/J 16wk old at time of launch) including eight from the Flight group and eight from the Ground Control group. From each group two liver samples were collected and frozen immediately after euthanasia (Flight mice dissected on-orbit after total 37 days after launch Samples FLT-M21 M22 and corresponding Ground Control samples GC-M31,M32). An additional six samples from each group were collected from frozen carcasses dissected post-flight (Samples FLT-M25,M26,M27,M28,M29,M30 and corresponding Ground Control samples GC-M35,M36,M37,M38,M39,M40). RNA-Seq whole genome and RNA BS-Seq (bisulfite sequencing) and proteomic expression profiling were performed.

Tags: 5mc-total-rna-methylation-analysis, dissection-condition, dna-library-construction, epitranscriptomics, extraction, gravitation, labeling, library-construction, mass-spectrometry, mass-spectrometry-protocol, nucleic-acid-extraction, nucleic-acid-sequencing, protein-extraction, proteomics-data-transformation, rna-library-construction, sample-collection
Organization: National Aeronautics and Space Administration
Global DNA methylation profiling of radiation-induced rat mammary carcinomas

To investigate DNA methylation patterns in rat mammary carcinomas induced by ionizing radiation. DNA methylation patterns were analyzed in radiation-induced rat mammary carcinomas and normal mammary gland tissues using CpG island microarray.

Tags: animal-husbandry, data-collection, dna-extraction, ionizing-radiation, labeling, mouse-habitat, normalization-data-transformation, nucleic-acid-hybridization, tissue, treatment-protocol
Organization: National Aeronautics and Space Administration
COHOWeb at the Space Physics Data Facility (SPDF)

Hourly-averaged deep space magnetic field, plasma, and ephemerides. This web service includes data from  missions including Helios1, Helios2, OMNI_M, Pioneer10, Pioneer11, Pioneer Venus, Stereo-A, Stereo-B, Ulysses, Voyager1, Voyager2, Mariner2, Pioneer6, Pioneer7. The Space Physics Data Facility (SPDF) is the archive of non-solar data for the Heliospheric Science Division (HSD) at NASA's Goddard Space Flight Center.

Tags: astrophysics, heliophysics, space-missions, space-physics, space-science
Organization: National Aeronautics and Space Administration
Swift-XRT Monitoring of Fermi-LAT Sources of Interest

The monitoring data shown on this site provides near real-time results from Swift observations of Fermi-LAT "sources of interest" and flaring sources. While this list is dominated by blazars, some X-ray binaries and Galactic transients are also included. The preliminary results on this page are intended to provide rapid feedback to the scientific community to aid in target of opportunity planning, multiwavelength campaigns, and planning of interesting scientific publications.

Tags: blazar, blazar-monitoring, mojave
Organization: National Aeronautics and Space Administration
Film Cooling Data

Researchers at NASA GRC conducted an experimental investigation of the flow physics of turbine film cooling by obtaining detailed mean and fluctuating velocity and temperature surveys in the turbulent jet flow field behind a row of large scale film cooling holes. Surface heat transfer film effectiveness and turbulent heat flux values were also determined.  Measurements were made in the NASA ERB SW-6 test facility, which consists of an 8.15-inch square open-inlet wind tunnel with a temperature-controlled secondary flow system. The test plate consisted of a three-hole array of film cooling holes that were fed from a plenum of different temperature.  The model geometry was scaled up roughly 30 times of typical turbine film cooling size to enable high spatial resolution measurements near the film coolant hole.  High-resolution survey data was taken along a centerline streamwise plane behind the hole as well as at various cross-sectional planes behind the hole at two blowing ratios.  Mean and fluctuating flow field velocity and temperature measurements were made using various thin wire anemometry probes; surface temperature and heat transfer measurements were made employing liquid crystal and infrared imaging techniques.  In addition, a two-plane particle image velocimetry (PIV) technique was performed downstream of the coolant holes to compare with the velocity and turbulent quantities measured by the hotwire probe.

Tags: anemometry, film-cooling, heat-flux, length-scale, temperature, turbulence, velocity
Organization: National Aeronautics and Space Administration
Rodent Research-1 (RR1) NASA Validation Flight: Mouse kidney transcriptomic proteomic and epigenomic data

NASA  s Rodent Research (RR) project is playing a critical role in advancing biomedical research on the physiological effects of space environments. Due to the limited resources for conducting biological experiments aboard the International Space Station (ISS) it is imperative to use crew time efficiently while maximizing high-quality science return. NASA  s GeneLab project has as its primary objectives to 1) further increase the value of these experiments using a multi-omics systems biology-based approach and 2) disseminate these data without restrictions to the scientific community. The current investigation assessed viability of RNA DNA and protein extracted from archived RR-1 tissue samples for epigenomic transcriptomic and proteomic assays. During the first RR spaceflight experiment a variety of tissue types were harvested from subjects snap-frozen or RNAlater-preserved and then stored at least a year at -80C after return to Earth. They were then prioritized for this investigation based on likelihood of significant scientific value for spaceflight research. All tissues were made available to GeneLab through the bio-specimen sharing program managed by the Ames Life Science Data Archive and included mouse adrenal glands quadriceps gastrocnemius tibialis anterior extensor digitorum longus soleus eye and kidney. We report here protocols for and results of these tissue extractions and thus the feasibility and value of these kinds of omics analyses. In addition to providing additional opportunities for investigation of spaceflight effects on the mouse transcriptome and proteome in new kinds of tissues our results may also be of value to program managers for the prioritization of ISS crew time for rodent research activities.

Tags: 5mc-total-rna-methylation-analysis, animal-and-tissue-sampling, animal-husbandry, data-transformation, dna-sequencing-library-construction, epitranscriptomics, genelab-rnaseq-data-processing-protocol, labeling, library-construction, mass-spectrometry, mouse-habitation, nucleic-acid-extraction, nucleic-acid-sequencing, protein-extraction, rna-sequencing-library-construction, sample-collection, spaceflight, tmt-isobaric-labeling
Organization: National Aeronautics and Space Administration
Navigation and Ancillary Information Facility's SPICE

The Navigation and Ancillary Information Facility (NAIF), acting under the directions of NASA's Planetary Science Division, has built an information system named 'SPICE' to assist NASA scientists in planning and interpreting scientific observations from space-borne instruments, and to assist NASA engineers involved in modeling, planning andexecuting activities needed to conduct planetary exploration missions The use of SPICE extends from mission concept development through the post-mission data analysis phase,including help with correlation of individual instrument data sets with those from other instruments on the same or on other spacecraft.

Tags: cvcm, events, materials, naif, navigation, outgassing, planetary-data-system, qualified, radiometry, spaceflight, technical-reports
Organization: National Aeronautics and Space Administration
Geometry, Grid and Boundary Condition Data for EEE Combustor and Turbine

The Energy Eﬃcient Engine (EEE) program was funded by NASA in the mid-1970s to develop a more eﬃcient turbofan engine for aircraft. The contractor reports and geometry of the EEE engines developed by GE and Pratt & Whitney are no longer proprietary and this is now public information. Under the NASA Transformational Tools and Technologies (TTT) project, NASA is using the EEE combustor and high-pressure turbine as a representative combustor/turbine combination to support development of fully-coupled combustor-turbine simulations using computational fluid dynamics (CFD). Geometry, CFD grid and CFD boundary condition files have been created based on publicly available information extracted from NASA contractor reports, such as NASA-CR-167980 (1982), NASA-CR-167955 (1982), NASA-CR-168274 (1984), NASA-CR-168289 (1984) and NASA-CR-168301 (1984). NASA is providing these geometry, grid and boundary condition files to other researchers to promote development of tools for the coupled analysis of aircraft combustors and high-pressure turbines.

Tags: nasa
Organization: National Aeronautics and Space Administration
Analysis of dust samples from the Russian part of the ISS

Our study focuses on the hardiest microorganisms inhabiting the ISS in order to assess their diversity and capabilities to resist certain stresses. We specifically selected dust samples from the Russian modules that were obtained 8-10 years ago and stored since then under sealed conditions on Earth. Targeting long-time survivors and spore-forming microorganisms we assessed consequently the cultivable microbial community of these samples in order to obtain model microbial strains that could help to analyze specific adaptation towards environmental stresses such as desiccation and lack of nutrients. In this study we analyzed these microorganisms with respect to their resistance towards thermal stress and exposure to clinically relevant antibiotics. In addition we assessed the bacterial and archaeal community via molecular methods (NGS sequencing) and compared our new data with the previously derived information from the ISS microbiome.

Tags: data-transformation, dust-extraction, genelab-amplicon-seq-data-processing-protocol, library-construction, nucleic-acid-extraction, nucleic-acid-sequencing, sample-collection, sample-location, spaceflight, treatment
Organization: National Aeronautics and Space Administration
Adaptive changes in the vestibular system of land snail to a 30-day spaceflight and readaptation on return to Earth

The vestibular system receives a permanent influence from gravity and reflexively controls equilibrium. If we assume gravity has remained constant during the species   evolution will its sensory system adapt to abrupt loss of that force? We address this question in the land snail Helix lucorum exposed to 30 days of near weightlessness aboard the Bion-M1 satellite and studied geotactic behavior of postflight snails differential gene expressions in statocyst transcriptome and electrophysiological responses of mechanoreceptors to applied tilts. Each approach revealed plastic changes in the snail  s vestibular system assumed in response to spaceflight. Absence of light during the mission also affected statocyst physiology as revealed by comparison to dark-conditioned control groups. Readaptation to normal tilt responses occurred at ~20 h following return to Earth. Despite the permanence of gravity the snail responded in a compensatory manner to its loss and readapted once gravity was restored.

Tags: animal-housing, food-deprivation, housing-animal, library-construction, light-cycle, nucleic-acid-extraction, nucleic-acid-sequencing, sample-collection, spaceflight, water-deprivation
Organization: National Aeronautics and Space Administration
Hindlimb suspension and reloading study

Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days and subsequent reloading for 1 day -Hindlimb suspension for 7 days and subsequent reloading for 7 days

Tags: data-collection, hindlimb-unloading, labeling, normalization-data-transformation, nucleic-acid-hybridization, rna-extraction, treatment-protocol
Organization: National Aeronautics and Space Administration