AndreaBozzo/ceres-open-data-index huggingface dataset:
Computational prediction of membrane-tethered transcription factors

Background
          Sequestration of transcription factors in the membrane is emerging as an important mechanism for the regulation of gene expression. A handful of membrane-spanning transcription factors has been previously identified whose access to the nucleus is regulated by proteolytic cleavage from the membrane. To investigate the existence of other transmembrane transcription factors, we analyzed computationally all proteins in SWISS-PROT/TrEMBL for the combined presence of a DNA-binding domain and a transmembrane segment.
        
        
          Results
          Using Pfam hidden Markov models and four transmembrane-prediction programs, we identified with high confidence 76 membrane-spanning transcription factors in SWISS-PROT/TrEMBL. Analysis of the distribution of two proteins predicted by our method, MTJ1 and DMRT2, confirmed their localization to intracellular membrane compartments. Furthermore, elimination of the predicted transmembrane segment led to nuclear localization for each of these proteins.
        
        
          Conclusions
          Our analysis uncovered a wealth of predicted membrane-spanning transcription factors that are structurally and taxonomically diverse, 56 of which lack experimental annotation. Seventy-five of the proteins are modular in structure, suggesting that a single proteolysis may be sufficient to liberate a DNA-binding domain from the membrane. This study provides grounds for investigations into the stimuli and mechanisms that release this intriguing class of transcription factors from membranes.

Tags: gene-expression, nih, protein-localization, transcription-factors, transmembrane-proteins
Organization: U.S. Department of Health & Human Services
Systematic review of the use of honey as a wound dressing

Objective
          To investigate topical honey in superficial burns and wounds though a systematic review of randomised controlled trials.
        
        
          Data sources
          Cochrane Library, MEDLINE, EMBASE, PubMed, reference lists and databases were used to seek randomised controlled trials. Seven randomised trials involved superficial burns, partial thickness burns, moderate to severe burns that included full thickness injury, and infected postoperative wounds.
        
        
          Review methods
          Studies were randomised trials using honey, published papers, with a comparator. Main outcomes were relative benefit and number-needed-to-treat to prevent an outcome relating to wound healing time or infection rate.
        
        
          Results
          One study in infected postoperative wounds compared honey with antiseptics plus systemic antibiotics. The number needed to treat with honey for good wound healing compared with antiseptic was 2.9 (95% confidence interval 1.7 to 9.7). Five studies in patients with partial thickness or superficial burns involved less than 40% of the body surface. Comparators were polyurethane film, amniotic membrane, potato peel and silver sulphadiazine. The number needed to treat for seven days with honey to produce one patient with a healed burn was 2.6 (2.1 to 3.4) compared with any other treatment and 2.7 (2.0 to 4.1) compared with potato and amniotic membrane. For some or all outcomes honey was superior to all these treatments. Time for healing was significantly shorter for honey than all these treatments. The quality of studies was low.
        
        
          Conclusion
          Confidence in a conclusion that honey is a useful treatment for superficial wounds or burns is low. There is biological plausibility.

Tags: burn-treatment, honey-wound-dressing, nih, systematic-review, wound-healing
Organization: U.S. Department of Health & Human Services
Compound 525059: Bis(trimethylsilyl)estradiol

Chemical compound data from PubChem database. IUPAC Name: trimethyl-[(13-methyl-3-trimethylsilyloxy-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthren-17-yl)oxy]silane. Molecular Formula: C24H40O2Si2. Molecular Weight: 416.7. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
2022 Uniform Reporting System (URS) Table For Nevada

This is the 2022 Uniform Reporting System (URS) Output Tables for Nevada. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-program, mental-health-services, national-outcome-measures, nevada, samhsa
Organization: U.S. Department of Health & Human Services
The new middle level health workers training in the Amhara regional state of Ethiopia: students' perspective

Background
          Following health sector reform, Ethiopia started training new categories of health workers. This study addresses students' perspectives regarding their training and career plans.
        
        
          Methods
          A cross sectional questionnaire was administered to 145 students in the three schools of the Amhara regional state of Ethiopia.
        
        
          Results
          The majority of students were male (62%) and originally from urban areas (76%). Job search was the most common reason for enrolling in the training for almost half (48%) of the respondents, followed by a desire to help the sick (46%). Once trained, the majority (98%) of graduates preferred to serve in the government sector and in rural health institutions (84%). Females were more willing to work in rural settings [χ2 (df 1)= 7.37; P = 0.007]. The majority (98%) of students felt the training period should be extended. 12% of graduates lacked confidence in their competencies after completing the training. A substantial proportion of the respondents (29%) did not feel the social science courses (Anthropology, Ecology and Psychology) were useful.
        
        
          Conclusions
          This study demonstrates that mid-level health professional students are highly motivated, wish to address the health needs of rural communities, and are interested in professional development. However, students do not feel the training programs are fully addressing their needs. The students found that the duration of the training, the time for theory and practice, the availability of teaching materials, the course contents and their teachers were inadequate. This study suggests that the current training programs have serious inadequacies that need to be addressed.

Tags: ethiopia-health, health-worker-training, nih, rural-health-workforce, student-perspectives
Organization: U.S. Department of Health & Human Services
Community Healthy Marriage Initiative Survey for Six Cities, 2007-2010

The Community Healthy Marriage Initiative (CHMI) evaluation was designed to evaluate community-level impacts of various relationship and marriage education programs. This study compared three sites which received grant funding from the Administration for Children and Families (ACF) (Dallas, Texas; St. Louis, Missouri; and Milwaukee, Wisconsin) with three cities that did not receive grant-funding (Fort Worth, Texas; Kansas City, Missouri; and Cleveland, Ohio) to determine what impacts grant funding has on these types of programs. 

<b>Units of Response: </b>CHMI program participants and non-CHMI program participants

<b>Type of Data: </b>Survey

<b>Tribal Data: </b>No

<b>COVID-19 Data: </b>No

<b>Periodicity: </b>One-time

<b>SORN: </b>https://www.federalregister.gov/documents/2015/04/02/2015-07440/privacy-act-of-1974-system-of-records-notice

<b>Data Use Agreement: </b>https://www.icpsr.umich.edu/rpxlogin

<b>Data Use Agreement Location: </b>https://www.icpsr.umich.edu/web/ICPSR/studies/34719/versions/V3/datadocumentation#

<b>Equity Indicators: </b>Ethnicity;Geographic Areas;Household Income;Household Size;Race;Sex 

<b>Granularity: </b>Individual;Program

<b>Spatial: </b>United States

<b>Geocoding: </b>Zip Code

Tags: children-and-families, community-service, grant-programs, research-and-evaluation
Organization: U.S. Department of Health & Human Services
2015 CMHS Uniform Reporting System (URS) Table for Tennessee

2015 Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for Tennessee. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, evidence-based-practices, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
The use of percentage change from baseline as an outcome in a controlled trial is statistically inefficient: a simulation study

Background
          Many randomized trials involve measuring a continuous outcome - such as pain, body weight or blood pressure - at baseline and after treatment. In this paper, I compare four possibilities for how such trials can be analyzed: post-treatment; change between baseline and post-treatment; percentage change between baseline and post-treatment and analysis of covariance (ANCOVA) with baseline score as a covariate. The statistical power of each method was determined for a hypothetical randomized trial under a range of correlations between baseline and post-treatment scores.
        
        
          Results
          ANCOVA has the highest statistical power. Change from baseline has acceptable power when correlation between baseline and post-treatment scores is high;when correlation is low, analyzing only post-treatment scores has reasonable power. Percentage change from baseline has the lowest statistical power and was highly sensitive to changes in variance. Theoretical considerations suggest that percentage change from baseline will also fail to protect from bias in the case of baseline imbalance and will lead to an excess of trials with non-normally distributed outcome data.
        
        
          Conclusions
          Percentage change from baseline should not be used in statistical analysis. Trialists wishing to report this statistic should use another method, such as ANCOVA, and convert the results to a percentage change by using mean baseline scores.

Tags: ancova, clinical-trials, nih, outcome-analysis, statistical-power
Organization: U.S. Department of Health & Human Services
CFSR Round 3 Statewide Data Indicators Workbook

This workbook provides state-by-state performance data for Round 3 of the Child and Family Services Reviews in addition to national performance comparisons for the past 12-month reporting period included in data profiles transmitted to states in February 2021.

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: child, data, policy, reporting, scope
Organization: U.S. Department of Health & Human Services
Lens epithelial cell apoptosis and intracellular Ca

Background
          Xanthurenic acid is an endogenous product of tryptophan degradation by indoleamine 2,3-dioxygenase (IDO). We have previously reported that IDO is present in mammalian lenses, and xanthurenic acid is accumulated in the lenses with aging. Here, we studied the involvement of xanthurenic acid in the human lens epithelial cell physiology.
        
        
          Methods
          Human lens epithelial cells primary cultures were used. Control cells, and cells in the presence of xanthurenic acid grow in the dark. Western blot analysis and immunofluorescence studies were performed.
        
        
          Results
          In the presence of xanthurenic acid human lens epithelial cells undergo apoptosis-like cell death. In the control cells gelsolin stained the perinuclear region, whereas in the presence of 10 μM xanthurenic acid gelsolin is translocated to the cytoskeleton, but does not lead to cytoskeleton breakdown. In the same condition caspase-3 activation, and DNA fragmentation was observed. At low (5 to 10 μM) of xanthurenic acid concentration, the elongation of the cytoskeleton was associated with migration of mitochondria and cytochrome c release. At higher concentrations xanthurenic acid (20 μM and 40 μM) damaged mitochondria were observed in the perinuclear region, and nuclear DNA cleavage was observed. We observed an induction of calpain Lp 82 and an increase of free Ca2+ in the cells in a xanthurenic acid concentration-dependent manner.
        
        
          Conclusions
          The results show that xanthurenic acid accumulation in human lens epithelial cells disturbs the normal cell physiology and leads to a cascade of pathological events. Xanthurenic acid induces calpain Lp82 and caspases in the cells growing in the dark and can be involved in senile cataract development.

Tags: calcium-signaling, cell-apoptosis, lens-epithelial-cells, nih, xanthurenic-acid
Organization: U.S. Department of Health & Human Services
Digitoxin medication and cancer; case control and internal dose-response studies

Background
          Digitoxin induces apoptosis in different human malignant cell lines in vitro. In this paper we investigated if patients taking digitoxin for cardiac disease have a different cancer incidence compared to the general population.
        
        
          Methods
          Computer stored data on digitoxin concentrations in plasma from 9271 patients with cardiac disease were used to define a user population. Age and sex matched controls from the Norwegian Cancer Registry were used to calculate the number of expected cancer cases.
        
        
          Results
          The population on digitoxin showed a higher incidence of cancer compared to the control population. However, an additional analysis showed that the population on digitoxin had a general increased risk of cancer already, before the start on digitoxin. Leukemia/lymphoma were the cancer types which stood out with the highest risk in the digitoxin population before starting on digitoxin. This indicates that yet unknown risk factors exist for cardiovascular disease and lymphoproliferative cancer.
          An internal dose-response analysis revealed a relationship between high plasma concentration of digitoxin and a lower risk for leukemia/lymphoma and for cancer of the kidney/urinary tract.
        
        
          Conclusion
          Morbidity and mortality are high in the population on digitoxin, due to high age and cardiac disease.These factors disturb efforts to isolate an eventual anticancer effect of digitoxin in this setting. Still, the results may indicate an anticancer effect of digitoxin for leukemia/lymphoma and kidney/urinary tract cancers. Prospective clinical cancer trials have to be done to find out if digitoxin and other cardiac glycosides are useful as anticancer agents.

Tags: cancer-incidence, cardiac-disease, case-control-study, digitoxin-medication, nih
Organization: U.S. Department of Health & Human Services
Family Group Decision-Making: Implementing the Family Group Conference

This Child Welfare Information Gateway Podcast is available on Apple Podcasts
  
  

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Metadata-only record linking to the original dataset. Open original dataset below.

Tags: child, family, making, policy, social
Organization: U.S. Department of Health & Human Services
Compound 525420: Disulfide, ethyl isopentyl

Chemical compound data from PubChem database. IUPAC Name: 1-(ethyldisulfanyl)-3-methylbutane. Molecular Formula: C7H16S2. Molecular Weight: 164.3. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
The relationship between the L1 and L2 domains of the insulin and epidermal growth factor receptors and leucine-rich repeat modules

Background
          Leucine-rich repeats are one of the more common modules found in proteins. The leucine-rich repeat consensus motif is LxxLxLxxNxLxxLxxLxxLxx- where the first 11–12 residues are highly conserved and the remainder of the repeat can vary in size Leucine-rich repeat proteins have been subdivided into seven subfamilies, none of which include members of the epidermal growth factor receptor or insulin receptor families despite the similarity between the 3D structure of the L domains of the type I insulin-like growth factor receptor and some leucine-rich repeat proteins.
        
        
          Results
          Here we have used profile searches and multiple sequence alignments to identify the repeat motif Ixx-LxIxx-Nx-Lxx-Lxx-Lxx-Lxx- in the L1 and L2 domains of the insulin receptor and epidermal growth factor receptors. These analyses were aided by reference to the known three dimensional structures of the insulin-like growth factor type I receptor L domains and two members of the leucine rich repeat family, porcine ribonuclease inhibitor and internalin 1B. Pectate lyase, another beta helix protein, can also be seen to contain the sequence motif and much of the structural features characteristic of leucine-rich repeat proteins, despite the existence of major insertions in some of its repeats.
        
        
          Conclusion
          Multiple sequence alignments and comparisons of the 3D structures has shown that right-handed beta helix proteins such as pectate lyase and the L domains of members of the insulin receptor and epidermal growth factor receptor families, are members of the leucine-rich repeat superfamily.

Tags: epidermal-growth-factor, insulin-receptor, leucine-rich-repeats, nih, protein-domains
Organization: U.S. Department of Health & Human Services
Compound 525671: p-Bromobenzylidene-nonyl-amine

Chemical compound data from PubChem database. IUPAC Name: 1-(4-bromophenyl)-N-nonylmethanimine. Molecular Formula: C16H24BrN. Molecular Weight: 310.27. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Biofilm formation by nontypeable

Background
          Nontypeable Haemophilus influenzae is an important cause of otitis media in children and lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). Several lines of evidence suggest that the bacterium grows as a biofilm in the human respiratory tract.
        
        
          Results
          Fifteen clinical isolates from middle ear fluid of children with otitis media and 15 isolates from sputum of adults with COPD were studied in an in vitro assay of biofilm formation. Striking variability among isolates was observed in their ability to form biofilms. Analysis of cell envelopes revealed minimal differences in banding patterns in polyacrylamide gels, alteration of expression of an epitope on lipooligosaccharide, and preservation of expression of selected epitopes on outer membrane proteins P2, P5 and P6 in biofilms compared to planktonically grown cells. A pilus-deficient variant showed a marked impairment in biofilm formation compared to its isogenic parent.
        
        
          Conclusions
          Nontypeable H. influenzae forms biofilms in vitro. Clinical isolates show substantial variability in their ability to grow as biofilms. Three major outer membrane proteins (P2, P5 and P6) are expressed during growth as a biofilm. Expression of lipooligosaccharide is altered during growth as a biofilm compared to planktonic growth. Pili are important in biofilm formation. As the role of biofilms in human infection becomes better defined, characterization of biofilms may be important in understanding the pathogenesis of infection and immune response to nontypeable H. influenzae in children with otitis media and adults with COPD.

Tags: biofilm-formation, copd-infection, haemophilus-influenzae, nih, otitis-media
Organization: U.S. Department of Health & Human Services
A case of central nervous system vasculitis related to an episode of Guillain-Barrè syndrome

The authors report their knowledge about an uncommon case of isolated vasculitis, restricted to the left sylvian artery during an auto-immune Guillain-Barrè syndrome (GBS), sustained by cytomegalovirus (CMV). An acute cardiopulmonary failure requiring a ventilator and vasopressor support manifested, notwithstanding plasma exchanging and immune-modulating therapy. An IgM-enriched formula administration coincided with a rapid amelioration of GBS and vasculitis to a complete recovery the next month after her discharge to a rehabilitation centre.

Tags: cns-vasculitis, cytomegalovirus-infection, guillain-barre-syndrome, igm-immunoglobulins, nih
Organization: U.S. Department of Health & Human Services
Need for critical care in gynaecology: a population-based analysis

Introduction
          The purpose of this study was to note potential gynaecological risk factors leading to intensive care and to estimate the frequency, costs and outcome of management.
        
        
          Materials and methods
          In a cross-sectional study of intensive care admissions in Kuopio from March 1993 to December 2000, 23 consecutive gynaecological patients admitted to a mixed medical-surgical intensive care unit (ICU) were followed. We recorded demographics, admitting diagnoses, scores on the Acute Physiological and Chronic Health Evaluation (APACHE) II, clinical outcome and treatment costs.
        
        
          Results
          The overall need for intensive care was 2.3 per 1000 women undergoing major surgery during the study period. Patients were 55.4 ± 16.9 (mean ± SD) years old, with a mean APACHE II score of 14.07 (± 5.57). The most common diagnoses at admission were postoperative haemorrhage (43%), infection (39%) and cardiovascular disease (30%). The duration of stay in the ICU was 4.97 (± 9.28) (range 1–42) days and the mortality within 6 months was 26%, although the mortality in the ICU was 0%. The total cost of intensive care was approximately US$7044 per patient.
        
        
          Conclusions
          Very few gynaecological patients develop complications requiring intensive care. The presence of gynaecological malignancy and pre-existing medical disorders are clinically useful predictors of eventual outcome, but many cases occur in women with a low risk and this implies that the risk is relevant to all procedures. Further research is needed to determine effective preventive approaches.

Tags: critical-care, gynecologic-surgery, icu-admission, nih, postoperative-hemorrhage
Organization: U.S. Department of Health & Human Services
2022 Uniform Reporting System (URS) Table For Wisconsin

This is the 2022 Uniform Reporting System (URS) Output Tables for Wisconsin. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-program, community-mental-health, mental-health-services, national-outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services