AndreaBozzo/ceres-open-data-index huggingface dataset:
Macrophages exposed continuously to lipopolysaccharide and other agonists that act via toll-like receptors exhibit a sustained and additive activation state

Background
          Macrophages sense microorganisms through activation of members of the Toll-like receptor family, which initiate signals linked to transcription of many inflammation associated genes. In this paper we examine whether the signal from Toll-like receptors [TLRs] is sustained for as long as the ligand is present, and whether responses to different TLR agonists are additive.
        
        
          Results
          RAW264 macrophage cells were doubly-transfected with reporter genes in which the IL-12p40, ELAM or IL-6 promoter controls firefly luciferase, and the human IL-1β promoter drives renilla luciferase. The resultant stable lines provide robust assays of macrophage activation by TLR stimuli including LPS [TLR4], lipopeptide [TLR2], and bacterial DNA [TLR9], with each promoter demonstrating its own intrinsic characteristics. With each of the promoters, luciferase activity was induced over an 8 hr period, and thereafter reached a new steady state. Elevated expression required the continued presence of agonist. Sustained responses to different classes of agonist were perfectly additive. This pattern was confirmed by measuring inducible cytokine production in the same cells. While homodimerization of TLR4 mediates responses to LPS, TLR2 appears to require heterodimerization with another receptor such as TLR6. Transient expression of constitutively active forms of TLR4 or TLR2 plus TLR6 stimulated IL-12 promoter activity. The effect of LPS, a TLR4 agonist, was additive with that of TLR2/6 but not TLR4, whilst that of lipopeptide, a TLR2 agonist, was additive with TLR4 but not TLR2/6. Actions of bacterial DNA were additive with either TLR4 or TLR2/6.
        
        
          Conclusions
          These findings indicate that maximal activation by any one TLR pathway does not preclude further activation by another, suggesting that common downstream regulatory components are not limiting. Upon exposure to a TLR agonist, macrophages enter a state of sustained activation in which they continuously sense the presence of a microbial challenge.

Tags: inflammatory-response, lps-stimulation, macrophage-activation, nih, toll-like-receptors
Organization: U.S. Department of Health & Human Services
Industries where health investment is growing

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Over $7.8 trillion is spent on healthcare globally. Nearly half of that total -- $3.5 trillion -- is spent in the U.S. With the healthcare sector growing significantly faster than the overall global economy, these numbers will almost certainly be much bigger by the end of the decade.

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Metadata-only record linking to the original dataset. Open original dataset below.

Tags: assessment, policy, real, scope, time
Organization: U.S. Department of Health & Human Services
2020 Uniform Reporting System (URS) Table For Puerto Rico

This is the 2020 Uniform Reporting System (URS) Output Tables for Puerto Rico. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-program, mental-health-services, national-outcome-measures, puerto-rico, samhsa
Organization: U.S. Department of Health & Human Services
2012 State Profile - Connecticut

This is a National Survey of Substance Abuse Treatment Services (N-SSATS) annual report showing the state profile for Connecticut in 2012. N-SSATS is designed to collect data on the location, characteristics, and use of alcohol and drug abuse treatment facilities and services throughout the 50 States, the District of Columbia, and other U.S. jurisdictions.

Tags: alcohol-drug-treatment, connecticut, n-ssats, samhsa, substance-abuse-treatment
Organization: U.S. Department of Health & Human Services
Weather conditions and sudden sensorineural hearing loss

Background
          Climatic or meteorological condition changes have been implicated in the pathogenesis of Idiopathic Sudden Sensorineural Hearing Loss (ISSHL). We investigated the seasonal distribution of ISSHL and evaluated the influence of meteorological parameters (such as temperature, humidity, and atmospheric pressure), their variation and covariation on the incidence of the disease.
        
        
          Methods
          A total of 82 cases of ISSHL, admitted to our department over a five-year period, were enrolled in the study. Seasonal distribution of the disease was investigated by dividing the year in four seasons. Meteorological data included daily values of 13 distinct parameters recorded at the meteorological station of the University of Ioannina during this period. A relationship between each meteorological variable and the incidence of ISSHL was investigated by applying (χ2) test on data from 13 contingency tables as well as by using logistic regression and t-test approaches. In addition, the influence of different weather types on the incidence of ISSHL was investigated using Cluster Analysis in order to create eight clusters (weather types) characteristic for the prefecture of Ioannina.
        
        
          Results
          The results of the study could not indicate any seasonal distribution of the disease. The incidence of ISSHL could not be significantly correlated either to any distinct meteorological parameter or to any specific weather type.
        
        
          Conclusions
          Meteorological conditions, such as those dominating in the Northwestern Greece, and/or their changes, have no proven effect on the incidence of ISSHL.

Tags: issshl-incidence, meteorological-parameters, nih, sensorineural-hearing-loss, weather-conditions
Organization: U.S. Department of Health & Human Services
Birth-Foster Parent Mentoring Teams

This Child Welfare Information Gateway Podcast is available on Apple Podcasts
  
  

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 website. Subscribe to receive new episodes as they are released.

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: care, child, foster, policy, sery
Organization: U.S. Department of Health & Human Services
Digital analysis of cDNA abundance; expression profiling by means of restriction fragment fingerprinting

Background
          Gene expression profiling among different tissues is of paramount interest in various areas of biomedical research. We have developed a novel method (DADA, Digital Analysis of cDNA Abundance), that calculates the relative abundance of genes in cDNA libraries.
        
        
          Results
          DADA is based upon multiple restriction fragment length analysis of pools of clones from cDNA libraries and the identification of gene-specific restriction fingerprints in the resulting complex fragment mixtures. A specific cDNA cloning vector had to be constructed that governed missing or incomplete cDNA inserts which would generate misleading fingerprints in standard cloning vectors. Double stranded cDNA was synthesized using an anchored oligo dT primer, uni-directionally inserted into the DADA vector and cDNA libraries were constructed in E. coli. The cDNA fingerprints were generated in a PCR-free procedure that allows for parallel plasmid preparation, labeling, restriction digest and fragment separation of pools of 96 colonies each. This multiplexing significantly enhanced the throughput in comparison to sequence-based methods (e.g. EST approach). The data of the fragment mixtures were integrated into a relational database system and queried with fingerprints experimentally produced by analyzing single colonies. Due to limited predictability of the position of DNA fragments on the polyacrylamid gels of a given size, fingerprints derived solely from cDNA sequences were not accurate enough to be used for the analysis. We applied DADA to the analysis of gene expression profiles in a model for impaired wound healing (treatment of mice with dexamethasone).
        
        
          Conclusions
          The method proved to be capable of identifying pharmacologically relevant target genes that had not been identified by other standard methods routinely used to find differentially expressed genes. Due to the above mentioned limited predictability of the fingerprints, the method was yet tested only with a limited number of experimentally determined fingerprints and was able to detect differences in gene expression of transcripts representing 0.05% of the total mRNA population (e.g. medium abundant gene transcripts).

Tags: cdna-abundance, expression-profiling, gene-expression, nih, restriction-fingerprinting
Organization: U.S. Department of Health & Human Services
2010-2011 NSDUH State Prevalence Estimates – Excel Tables

This page presents the table of contents of excel tables of State estimates for measures of substance use and mental health based on the combined 2009 and 2010 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Results are provided by age group.

Tags: mental-health, nsduh, samhsa, state-prevalence, substance-use
Organization: U.S. Department of Health & Human Services
2012 State Profile - Washington

This is a National Survey of Substance Abuse Treatment Services (N-SSATS) annual report showing the state profile for Washington in 2012. N-SSATS is designed to collect data on the location, characteristics, and use of alcohol and drug abuse treatment facilities and services throughout the 50 States, the District of Columbia, and other U.S. jurisdictions.

Tags: addiction-services, n-ssats, samhsa, substance-abuse-treatment, washington-state
Organization: U.S. Department of Health & Human Services
2012 NSDUH Mental Health Surveillance Study Design and Estimation Report

This report describes the design and estimation procedures to the supplemental Mental Health Surveillance Study (MHSS) data collection to the 2012 National Survey on Drug Use and Health (NSDUH). The report describes instruments used in measuring mental illness, sample design and selection (including the MHSS clinical sample characteristics), data collection and processing, weighting, nonresponse adjustment, and the development of the model for estimating mental illness.

Tags: mental-health-surveillance, mental-illness-estimation, nsduh-mhss, samhsa, survey-methodology
Organization: U.S. Department of Health & Human Services
America's Need for and Receipt of Substance Use Treatment in 2015

This report uses 2015 National Survey on Drug Use and Health (NSDUH) data to examine the need for and receipt of substance use treatment at a specialty facility among people aged 12 or older. This report also examines perceived need for substance use treatment. Comparisons are made between adolescents aged 12 to 17, young adults aged 18 to 25, and adults aged 26 or older.

Tags: nsduh-data, perceived-need, samhsa, substance-use-treatment, treatment-need
Organization: U.S. Department of Health & Human Services
Poly purine.pyrimidine sequences upstream of the beta-galactosidase gene affect gene expression in

Background
          Poly purine.pyrimidine sequences have the potential to adopt intramolecular triplex structures and are overrepresented upstream of genes in eukaryotes. These sequences may regulate gene expression by modulating the interaction of transcription factors with DNA sequences upstream of genes.
        
        
          Results
          A poly purine.pyrimidine sequence with the potential to adopt an intramolecular triplex DNA structure was designed. The sequence was inserted within a nucleosome positioned upstream of the β-galactosidase gene in yeast, Saccharomyces cerevisiae, between the cycl promoter and gal 10Upstream Activating Sequences (UASg). Upon derepression with galactose, β-galactosidase gene expression is reduced 12-fold in cells carrying single copy poly purine.pyrimidine sequences. This reduction in expression is correlated with reduced transcription. Furthermore, we show that plasmids carrying a poly purine.pyrimidine sequence are not specifically lost from yeast cells.
        
        
          Conclusion
          We propose that a poly purine.pyrimidine sequence upstream of a gene affects transcription. Plasmids carrying this sequence are not specifically lost from cells and thus no additional effort is needed for the replication of these sequences in eukaryotic cells.

Tags: beta-galactosidase, gene-expression, nih, saccharomyces-cerevisiae, triplex-dna
Organization: U.S. Department of Health & Human Services
Compound 528449: 1-Hexadecene, 10-ethyl

Chemical compound data from PubChem database. IUPAC Name: 10-ethylhexadec-1-ene. Molecular Formula: C18H36. Molecular Weight: 252.5. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Comprehensive Child Welfare Information System (CCWIS) and Software Bills of Materials (SBOMs)

This Information Memorandum (IM) provides title IV-E agencies that choose to develop a CCWIS with information on SBOMs.  Agencies can use SBOMs to enhance CCWIS security by supporting source code integrity and software supply chain security.  This IM provides information and resources applicable to federal information technology (IT) procurements.  While the Children’s Bureau (CB) does not currently require a SBOM for state and tribal CCWIS procurements, we strongly encourage title IV-E agencies to require a SBOM with CCWIS procurements and contracts to increase IT security.

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: child, guidance, policy, real, time
Organization: U.S. Department of Health & Human Services
California: 2011-2012 NSDUH

This report presents California\'s estimates for 25 measures of substance use and mental health based on the combined 2011 and 2012 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Resuts are provided by State and for five age groups: 12 to 17, 18 to 25, 26 or older, 18 or older and all persons 12 or older.

Tags: california, mental-health, nsduh, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
2013 NSSATS - Data on Substance Abuse Treatment Facilities

This is a National Survey of Substance Abuse Treatment Services (N-SSATS) annual report showing  data on substance abuse treatment facilities in 2013. N-SSATS is designed to collect data on the location, characteristics, and use of alcohol and drug abuse treatment facilities and services throughout the 50 States, the District of Columbia, and other U.S. jurisdictions.

Tags: addiction-services, behavioral-health, samhsa, substance-abuse, treatment-facilities
Organization: U.S. Department of Health & Human Services
Compound 525517: 2-(3-Chloro-4-benzyloxy-phenyl)-propionic acid, methyl ester

Chemical compound data from PubChem database. IUPAC Name: methyl 2-(3-chloro-4-phenylmethoxyphenyl)propanoate. Molecular Formula: C17H17ClO3. Molecular Weight: 304.8. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
State and Substate Estimates of Any Mental Illness from the 2012–2014 National Surveys on Drug Use and Health

This report provides state and substate estimates of any mental illness among adults aged 18 or older using combined 2012-2014 National Survey on Drug Use and Health data. Estimates are provided for the nation as a whole and for each state and substate areas. Comparisons are made to estimates from 2010–2012.

Tags: adult-mental-illness, mental-health, nsduh-data, samhsa, substate-estimates
Organization: U.S. Department of Health & Human Services
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to relatively large errors in a significant proportion of samples tested.

Tags: gene-expression, housekeeping-genes, nih, normalization-methods, rt-pcr
Organization: U.S. Department of Health & Human Services
Pulse pressure and age at menopause

Background
          The objective of this study was to study the association of early age at menopause with pulse pressure (PP), a marker of arterial stiffness, and PP change.
        
        
          Methods
          The effect of natural menopause was studied in 2484 women from the Atherosclerosis Risk in Communities (ARIC) Study who had not used hormone replacement therapy and who had not had a hysterectomy. The cross-sectional association of age with PP was evaluated in the entire cohort. The cross-sectional association of recalled age at menopause was evaluated in the 1688 women who were postmenopausal at baseline. PP change over 6 years was assessed in relation to menopausal age separately in women who were postmenopausal at baseline and in those whose menopause occurred during the 6-year interval.
        
        
          Results
          Chronological age was strongly and positively associated with PP in cross-sectional analyses, but not independently associated with PP change. While menopausal age was not associated cross-sectionally with PP, early age at menopause (age<45) was significantly and independently associated with a slightly larger increase in PP (8.4, 95% CI 7.0–9.8) than later menopause (6.5, 95% CI 5.8;7.2). However, among normotensive women the difference was not statistically significant (p = 0.07, 6.1 vs 4.7).
        
        
          Conclusions
          Early age at menopause may be related to a greater increase in arterial stiffness, but the effect appears to be small and further evidence is needed.

Tags: age-at-menopause, aric-study, arterial-stiffness, nih, pulse-pressure
Organization: U.S. Department of Health & Human Services
Statins as modulators of bone formation

The use of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) to reduce serum cholesterol is well described. However, the recent finding that statins have direct effects on bone was unexpected. A number of epidemiological studies have recently been published that explore the effects of statins on bone mineral density and risk of fracture in humans. Statins may act by directly stimulating the expression of bone morphogenetic protein-2 and increasing osteoblast differentiation or, like nitrogen-containing bisphosphonates, may have effects on the mevalonate pathway that leads to inhibition of osteoclast activity and osteoblast apoptosis. In addition, the demonstration that statins can inhibit inflammation and encourage angiogenesis offers other possibilities for action.

Tags: bone-mineral-density, fracture-risk, nih, osteoblast-differentiation, statin-therapy
Organization: U.S. Department of Health & Human Services
Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase

Background
          Epstein-Barr virus (EBV) infects resting B-lymphocytes and transforms them into immortal proliferating lymphoblastoid cell lines (LCLs) in vitro. The transformed immunoblasts may grow up as immunoblastic lymphomas in immuno-suppressed hosts.
        
        
          Results
          In order to identify cellular protein targets that may be involved in Epstein-Barr virus mediated B-cell transformation, human LCL cDNA library was screened with one of the transformation associated nuclear antigens, EBNA-3 (also called EBNA-3A), using the yeast two-hybrid system. A clone encoding a fragment of a novel human protein was isolated (clone 538). The interaction was confirmed using in vitro binding assays. A full-length cDNA clone (F538) was isolated. Sequence alignment with known proteins and 3D structure predictions suggest that F538 is a novel human uridine kinase/uracil phosphoribosyltransferase. The GFP-F538 fluorescent fusion protein showed a preferentially cytoplasmic distribution but translocated to the nucleus upon co-expression of EBNA-3. A naturally occurring splice variant of F538, that lacks the C-terminal uracil phosphoribosyltransferase part but maintain uridine kinase domain, did not translocate to the nucleus in the presence of EBNA3. Antibody that was raised against the bacterially produced GST-538 protein showed cytoplasmic staining in EBV negative Burkitt lymphomas but gave a predominantly nuclear staining in EBV positive LCL-s and stable transfected cells expressing EBNA-3.
        
        
          Conclusion
          We suggest that EBNA-3 by direct protein-potein interaction induces the nuclear accumulation of a novel enzyme, that is part of the ribonucleotide salvage pathway. Increased intranuclear levels of UK/UPRT may contribute to the metabolic build-up that is needed for blast transformation and rapid proliferation.

Tags: b-lymphocyte-transformation, ebna-3, epstein-barr-virus, nih, uridine-kinase
Organization: U.S. Department of Health & Human Services
2011 CMHS Uniform Reporting System (URS) Output Tables for Minnesota

Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for 2011. The Uniform Reporting System (URS) is a state and national reporting system collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, mental-health-services, national-outcome-measures, samhsa, substance-abuse
Organization: U.S. Department of Health & Human Services