AndreaBozzo/ceres-open-data-index huggingface dataset:
Activation of the peroxisome proliferator-activated receptor α protects against myocardial ischaemic injury and improves endothelial vasodilatation

Background
          The peroxisome proliferator-activated receptor α (PPARα) plays an important role in the metabolism of lipoproteins and fatty acids, and seems to protect against the development of atherosclerosis. To evaluate the possible protective role of PPARα on cardiovascular function, the effect of the PPARα agonist, fenofibrate was assessed with respect to ischaemia/reperfusion injury and endothelial function in mice.
        
        
          Results
          Fenofibrate treatment reduces myocardial infarction size and improves post-ischaemic contractile dysfunction. Hearts from PPARα null mice exhibit increased susceptibility to ischaemic damages and were refractory to protection by fenofibrate treatment suggesting that the beneficial effects of fenofibrate were mediated via PPARα. Furthermore, fenofibrate improves endothelium- and nitric oxide-mediated vasodilatation in aorta and mesenteric vascular bed. A decreased inhibitory effect of reactive oxygen species in the vessel wall accounts for enhanced endothelial vasodilatation. However, the latter cannot be explained by an increase in nitric oxide synthase expression nor by an increase sensitivity of the arteries to nitric oxide.
        
        
          Conclusions
          Altogether the present data suggest that fenofibrate exerts cardioprotective effect against ischaemia and improves nitric oxide-mediated response probably by enhancing antioxidant capacity of the vessel wall. These data underscore new therapeutic perspectives for PPARα agonists in ischaemic myocardial injury and in cardiovascular diseases associated with endothelial dysfunction.

Tags: endothelial-vasodilation, fenofibrate-treatment, myocardial-ischemia, nih, ppar-alpha
Organization: U.S. Department of Health & Human Services
2017 Uniform Reporting System (URS) Table for Louisiana

This is the 2017 Uniform Reporting System (URS) Output Tables for Louisiana. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: block-grant-program, mental-health-services, national-outcome-measures, samhsa, substance-abuse-treatment
Organization: U.S. Department of Health & Human Services
Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25

Background
          The ubiquitin-dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitinating enzymes constitute a complex protein family involved in a multitude of cellular processes. The ubiquitin-specific proteases (UBP) are a group of enzymes whose predicted function is to reverse the ubiquitinating reaction by removing ubiquitin from a large variety of substrates. We have lately reported the characterization of human USP25, a specific-ubiquitin protease gene at 21q11.2, with a specific pattern of expression in murine fetal brains and adult testis.
        
        
          Results
          Database homology searches at the DNA and protein levels and cDNA library screenings led to the identification of a new UBP member in the human genome, named USP28, at 11q23. This novel gene showed preferential expression in heart and muscle. Moreover, cDNA, expressed sequence tag and RT-PCR analyses provided evidence for alternatively spliced products and tissue-specific isoforms. Concerning function, USP25 overexpression in Down syndrome fetal brains was shown by real-time PCR.
        
        
          Conclusions
          On the basis of the genomic and protein sequence as well as the functional data, USP28 and USP25 establish a new subfamily of deubiquitinating enzymes. Both genes have alternatively spliced exons that could generate protein isoforms with distinct tissue-specific activity. The overexpression of USP25 in Down syndrome fetal brains supports the gene-dosage effects suggested for other UBP members related to aneuploidy syndromes.

Tags: alternative-splicing, deubiquitinating-enzymes, nih, usp25-expression, usp28-isoforms
Organization: U.S. Department of Health & Human Services
Evidence for large domains of similarly expressed genes in the

Background
          Transcriptional regulation in eukaryotes generally operates at the level of individual genes. Regulation of sets of adjacent genes by mechanisms operating at the level of chromosomal domains has been demonstrated in a number of cases, but the fraction of genes in the genome subject to regulation at this level is unknown.
        
        
          Results
          Drosophila gene-expression profiles that were determined from over 80 experimental conditions using high-density oligonucleotide microarrays were searched for groups of adjacent genes that show similar expression profiles. We found about 200 groups of adjacent and similarly expressed genes, each having between 10 and 30 members; together these groups account for over 20% of assayed genes. Each group covers between 20 and 200 kilobase pairs of genomic sequence, with a mean group size of about 100 kilobase pairs. Groups do not appear to show any correlation with polytene banding patterns or other known chromosomal structures, nor were genes within groups functionally related to one another.
        
        
          Conclusions
          Groups of adjacent and co-regulated genes that are not otherwise functionally related in any obvious way can be identified by expression profiling in Drosophila. The mechanism underlying this phenomenon is not yet known.

Tags: drosophila-melanogaster, gene-expression, genomic-sequence, microarray-data, nih
Organization: U.S. Department of Health & Human Services
Malarone treatment failure and

We report the first in vitro and genetic confirmation of Malarone® (GlaxoSmithKline; atovaquone and proguanil hydrochloride) resistance in Plasmodium falciparum acquired in Africa. On presenting with malaria two weeks after returning from a 4-week visit to Lagos, Nigeria without prophylaxis, a male patient was given a standard 3-day treatment course of Malarone®. Twenty-eight days later the parasitaemia recrudesced. Parasites were cultured from the blood and the isolate (NGATV01) was shown to be resistant to atovaquone and the antifolate pyrimethamine. The cytochrome b gene of isolate NGATV01 showed a single mutation, Tyr268Asn which has not been seen previously.

Tags: antimalarial-resistance, cytochrome-b-mutation, malarone-resistance, nih, plasmodium-falciparum
Organization: U.S. Department of Health & Human Services
Supervised harvesting of expression trees

Background
          We propose a new method for supervised learning from gene expression data. We call it 'tree harvesting'. This technique starts with a hierarchical clustering of genes, then models the outcome variable as a sum of the average expression profiles of chosen clusters and their products. It can be applied to many different kinds of outcome measures such as censored survival times, or a response falling in two or more classes (for example, cancer classes). The method can discover genes that have strong effects on their own, and genes that interact with other genes.
        
        
          Results
          We illustrate the method on data from a lymphoma study, and on a dataset containing samples from eight different cancers. It identified some potentially interesting gene clusters. In simulation studies we found that the procedure may require a large number of experimental samples to successfully discover interactions.
        
        
          Conclusions
          Tree harvesting is a potentially useful tool for exploration of gene expression data and identification of interesting clusters of genes worthy of further investigation.

Tags: cancer-research, gene-expression, lymphoma, nih, supervised-learning
Organization: U.S. Department of Health & Human Services
Compound 528328: Furfuryl 2-propenyl sulfide

Chemical compound data from PubChem database. IUPAC Name: 1-(prop-2-enylsulfanylmethyl)cyclopenta-1,3-diene. Molecular Formula: C9H12S. Molecular Weight: 152.26. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Compound 527378: 20,26-Dimethyl-nonacosyl cyanide

Chemical compound data from PubChem database. IUPAC Name: 21,27-dimethyltriacontanenitrile. Molecular Formula: C32H63N. Molecular Weight: 461.8. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Compound 525405: 2-Methyl-3,5-dithianonane

Chemical compound data from PubChem database. IUPAC Name: 1-(propan-2-ylsulfanylmethylsulfanyl)butane. Molecular Formula: C8H18S2. Molecular Weight: 178.4. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Release of annexin V-binding membrane microparticles from cultured human umbilical vein endothelial cells after treatment with camptothecin

Background
          Elevated plasma counts of endothelial microparticles (MP) have been demonstrated in various diseases with a vascular injury component. We used flow cytometry to study the MP-release from cultured human umbilical vein endothelial cells (HUVEC) stimulated by various agonists. MP-release by a topoisomerase I inhibitor camptothecin has been studied in detail.
        
        
          Results
          Overnight stimulation of HUVEC with either LPS or TNFα, or 30 min stimulation with thrombin, phorbol-myristate-acetate, tissue plasminogen activator, or angiotensin-II did not cause a significant release of annexin V-binding MP. In contrast, induction of apoptosis with 5 μM camptothecin, documented by 60–70% desquamation of HUVEC culture, annexin V-binding to the cells and DNA-fragmentation, led to a release of annexin V-binding microparticles (~80,000 MP/103 cells). This microparticle-release was prevented by Z-Val-Ala-Asp-fluoromethyl-ketone (ZVAD). Lower concentration of camptothecin (500 nM) induced comparable microparticle-release without loss of the culture confluence and without increase in annexin V-binding to the cells or DNA-fragmentation. Analyzed microparticles were free of nucleic acids and 95% of microparticles were 0.3–1 μm in size. Double-labeling flow cytometry assay showed that all annexin V-binding Microparticles expressed CD59 but only approximately 50% of these also expressed CD105.
        
        
          Conclusions
          Camptothecin treated HUVEC released different populations of annexin V-binding membrane microparticles at early stage after proapoptotic stimulation before detection of phosphatidylserine exposure on the cells or DNA fragmentation. The microparticle-release was ZVAD sensitive but was not enhanced at the executive phase of apoptosis. These observations offer a new insight into microparticle-release as a marker of endothelial stimulation and injury.

Tags: annexin-v-binding, camptothecin-induced-apoptosis, endothelial-microparticles, huvec-cells, nih
Organization: U.S. Department of Health & Human Services
NSDUH 2017 Person-Level Sampling Weight Calibration Report

This report contains a brief review of the sampling weight calibration methodology used for the 2017 National Survey on Drug Use and Health (NSDUH). This report also lists detailed documentation on the implementation steps and evaluation results from the weight calibration application. The constrained exponential modeling (CEM) method used in the surveys before 1999 (referred to in this report as the generalized exponential model [GEM]) was modified to provide more flexibility in dealing internally with the extreme weights and for setting bounds directly on the weight adjustment factors so they can become suitable for nonresponse (nr) and poststratification (ps) adjustments.

Tags: nsduh, samhsa, sampling-weights, substance-use, survey-methodology
Organization: U.S. Department of Health & Human Services
Publications Using SAMHSA Data2012 Uniform Reporting System (URS) Tables for Federated States of Micronesia

Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for 2012. The Uniform Reporting System (URS) is a state and national reporting system collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, mental-health-services, national-outcome-measures, samhsa, urs-data
Organization: U.S. Department of Health & Human Services
Behavioral Health Barometer: Georgia, 2015

This is a behavioral health report for the state of Georgia. The report utilizes data from the National Survey on Drug Use and Health (NSDUH), and the Uniform Reporting System. The topics addressed in the report include youth substance use, youth mental health and treatment, mental health and treatment, substance use and substance use treatment.

Tags: georgia-behavioral-health, mental-health-treatment, samhsa, substance-use-disorders, youth-substance-use
Organization: U.S. Department of Health & Human Services
District of Columbia (DC)

This report uses 2008 to 2010 National Survey on Drug Use and Health (NSDUH) to assess past year alcohol use disorder and illicit drug use disorder among persons aged 12 or older for the District of Columbia overall and for substate regions within the District of Columbia. Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE).

Tags: alcohol-abuse, mental-health-dc, nsduh-data, samhsa, substance-use-disorder
Organization: U.S. Department of Health & Human Services
2014 CMHS Uniform Reporting System (URS) Table for Montana

2014 Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for Montana. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: community-mental-health, mental-health-services, montana, outcome-measures, samhsa
Organization: U.S. Department of Health & Human Services
The vitamin D receptor polymorphism in the translation initiation codon is a risk factor for insulin resistance in glucose tolerant Caucasians

Background
          Although vitamin D receptor (VDR) polymorphisms have been shown to be associated with abnormal glucose metabolism, the reported polymorphisms are unlikely to have any biological consequences. The VDR gene has two potential translation initiation sites. A T-to-C polymorphism has been noted in the first ATG (f allele), abolishing the first translation initiation site and resulting in a peptide lacking the first three amino acids (F allele). We examined the role of this polymorphism in insulin sensitivity and beta cell function. This study included 49 healthy Caucasian subjects (28 females, age 28 ± 1 years old, body mass index 24.57 ± 0.57 kg/m2, waist-hip ratio 0.81 ± 0.01 cm/cm). They were all normotensive (less than 140/90 mmHg) and glucose tolerant, which was determined by a standard 75-gm oral glucose tolerance test. Their beta cell function (%B) and insulin sensitivity (%S) were calculated based on the Homeostasis Model Assessment (HOMA). Their genotypes were determined by a polymerase chain reaction-restriction fragment length polymorphism analysis. Phenotypes were compared between genotypic groups.
        
        
          Results
          There were 18 FF, 21 Ff, and 10 ff subjects. Since only 10 ff subjects were identified, they were pooled with the Ff subjects during analyses. The FF and Ff/ff groups had similar glucose levels at each time point before and after a glucose challenge. The Ff/ff group had higher insulin levels than the FF group at fasting (P=0.006), 30 minutes (P=0.009), 60 minutes (P=0.049), and 90 minutes (P=0.042). Furthermore, the Ff/ff group also had a larger insulin area under the curve than the FF group (P=0.009). While no difference was noted in %B, the Ff/ff group had a lower %S than the FF group (0.53 vs. 0.78, P=0.006). A stepwise regression analysis confirmed that the Fok I polymorphism was an independent determinant for %S, accounting for 29.3% of variation in %S when combined with waist-hip ratio.
        
        
          Conclusions
          We report that the Fok I polymorphism at the VDR gene locus is associated with insulin sensitivity, but has no influence on beta cell function in healthy Caucasians. Although this polymorphism has been shown to affect the activation of vitamin D-dependent transcription, the molecular basis of the association between this polymorphism and insulin resistance remains to be determined.

Tags: genetic-polymorphism, glucose-tolerance, insulin-resistance, nih, vitamin-d-receptor
Organization: U.S. Department of Health & Human Services
Evaluation of Imputation Methods for the National Survey on Drug Use and Health

Although PMN imputation as currently implemented has a number of advantages, including the ability to use a large number of similar variables to determine the imputed value and to provide individual record consistency among very complex variable relationships, the goal of this study was to evaluate this method compared with other options, especially in the context of the redesign of the NSDUH.

Tags: imputation-methods, nsduh, samhsa, substance-use, survey-methodology
Organization: U.S. Department of Health & Human Services
Compound 525638: N,N-Dimethyl-N'-octyl-benzamidine

Chemical compound data from PubChem database. IUPAC Name: N,N-dimethyl-N'-octylbenzenecarboximidamide. Molecular Formula: C17H28N2. Molecular Weight: 260.4. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
NYTD Technical Assistance Brief No. 11

The brief discusses the requirements for reporting NYTD data element 3 (record number) and common mistakes states make in reporting the same record number to NYTD and AFCARS for a youth who is or was in foster care.

Metadata-only record linking to the original dataset. Open original dataset below.

Tags: care, foster, guidance, policy, reporting
Organization: U.S. Department of Health & Human Services
How many replicates of arrays are required to detect gene expression changes in microarray experiments? A mixture model approach

Background
          It has been recognized that replicates of arrays (or spots) may be necessary for reliably detecting differentially expressed genes in microarray experiments. However, the often-asked question of how many replicates are required has barely been addressed in the literature. In general, the answer depends on several factors: a given magnitude of expression change, a desired statistical power (that is, probability) to detect it, a specified Type I error rate, and the statistical method being used to detect the change. Here, we discuss how to calculate the number of replicates in the context of applying a nonparametric statistical method, the normal mixture model approach, to detect changes in gene expression.
        
        
          Results
          The methodology is applied to a data set containing expression levels of 1,176 genes in rats with and without pneumococcal middle-ear infection. We illustrate how to calculate the power functions for 2, 4, 6 and 8 replicates.
        
        
          Conclusions
          The proposed method is potentially useful in designing microarray experiments to discover differentially expressed genes. The same idea can be applied to other statistical methods.

Tags: gene-expression, microarray-analysis, nih, pneumococcal-infection, statistical-power
Organization: U.S. Department of Health & Human Services
2013 NSDUH Dependence, Abuse, and Treatment Tables (Standard Errors and P Values) - 5.1 to 5.56

These detailed tables present standard errors for totals and prevalence estimates of substance dependence/abuse and treatment need/receipt from the 2013 National Survey on Drug Use and Health (NSDUH). Substances examined include illicit drugs, marijuana, cocaine, heroin, hallucinogens, inhalants, and the nonmedical use of prescription-type pain relievers, tranquilizers, stimulants, and sedatives, and alcohol.Standard errors are provided by age group, gender, race/ethnicity, education level, employment status, and geographic area. Standard errors are provided for both 2013 and 02012.

Tags: nsduh-data, samhsa, standard-errors, substance-abuse, treatment-need
Organization: U.S. Department of Health & Human Services
Driving Under the Influence of Alcohol and Illicit Drugs

This short report uses 2002 to 2014 National Survey on Drug Use and Health (NSDUH) to assess trends in driving under the influence of illicit drugs or alcohol in the United States among those aged 16 or older, by age and gender.

Tags: dui-alcohol-drugs, impaired-driving, nsduh-data, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Compound 525058: N-methoxy-10,13-dimethyl-3,11-bis(trimethylsilyloxy)-1,2,3,4,5,6,7,8,9,11,12,14,15,16-tetradecahydrocyclopenta[a]phenanthren-17-imine

Chemical compound data from PubChem database. IUPAC Name: N-methoxy-10,13-dimethyl-3,11-bis(trimethylsilyloxy)-1,2,3,4,5,6,7,8,9,11,12,14,15,16-tetradecahydrocyclopenta[a]phenanthren-17-imine. Molecular Formula: C26H49NO3Si2. Molecular Weight: 479.8. This dataset contains comprehensive chemical information including structural data, physical properties, and biological activities. Useful for drug discovery, chemical research, and educational purposes.

Tags: chemistry, compound, pubchem, research, science
Organization: U.S. Department of Health & Human Services
Treatment Episode Data Set (TEDS) 2002-2012: National Admissions to Substance Abuse Treatment Services

This report presents national-level data from the TEDS for admissions in 2012 and trend data for 2002 to 2012. The report provides information on the demographic and substance abuse characteristics of admissions to treatment aged 12 and older for abuse of alcohol and/or drugs in facilities that report to individual state administrative data systems.

Tags: alcohol-drug-abuse, samhsa, substance-abuse-treatment, teds, treatment-admissions
Organization: U.S. Department of Health & Human Services
Instructions to Update the SAMHSA SMI and SED Estimates URS Table 1: Number of Adults with Serious Mental Illness, Age 18 and Older, by State, 2018

This is a Uniform Reporting System (URS) series of tables that details the number of adults with serious mental illness (SMI), ages 18 and older, by State, from 2018.

Tags: adult-mental-health, samhsa, serious-mental-illness, smi-estimates, state-level-data
Organization: U.S. Department of Health & Human Services