AndreaBozzo/ceres-open-data-index huggingface dataset:
Two novel frame shift, recurrent and

We have identified four different mutations causing leukocyte
adhesion deficiency (LAD) in the ITGB2 gene of patients from a highly inbred population. Two were novel single-bp deletions (1497delG and 1920delG) causing frame shift and the two others were the missense mutations G284S and R593C. In our study, the G284S was a recurrent mutation while the R593C occurred de novo. We have also characterized a novel Xba1 polymorphic site located at the 5′ end of the ITGB2 locus. Family studies showed that the 1497delG mutation segregated with this marker and the intragenic AvaII polymorphic marker, suggesting the presence of a founder effect. The observation of a
heterogeneous spectrum including de novo and recurrent mutations causing LAD in a highly inbred population is rather unexpected. In view of the literature published on the molecular genetics of LAD and considering the ethnic origin of the patients studied, our findings confirm the heterogeneity of the mutations causing LAD and point out potential mutational hot spots in the ITGB2 gene.

Tags: frameshift-mutation, genetic-polymorphism, itgb2-gene, leukocyte-adhesion-deficiency, nih
Organization: U.S. Department of Health & Human Services
Seattle: Drug-Related Emergency Department Visits, 2004-2011 - All Alcohol

Excel file with estimates, rates, relative standard errors, trends and confidence intervals, covering 2004-2011 for emergency department visits involving alcohol in the Seattle metropolitan area.  For adults aged 21 and older, the alcohol was found in combination with other drugs. For patients under the age of 21, the visit may involve alcohol alone or in combination with other drugs.File includes data for over 500 drugs, including illicit drugs, prescription and over-the-counter medications, dietary supplements, inhalants and alcohol.  Includes total visits, gender, age group, race/ethnicity, outcome of visit, drug combinations, number of drugs involved in visit, drug frequency, and drugs confirmed by toxicology.Additional detail is provided for illicit drugs, psychotherapeutic drugs, central nervous system (CNS) drugs, respiratory drugs and cardiovascular drugs.

Tags: alcohol-related-ed, drug-overdose, samhsa, seattle-metro, substance-abuse
Organization: U.S. Department of Health & Human Services
Cigarette Smoking Decreases among All Adults Except Those Aged 26 to 34 (2002 to 2011 NSDUH)

This report uses 2002 and 2011 National Survey on Drug Use and Health (NSDUH) to assess past month cigarette use among adults aged 18 or older. Results are shown by year and age group.

Tags: adult-health, cigarette-smoking, samhsa, substance-use, tobacco-use
Organization: U.S. Department of Health & Human Services
Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes

Background
          Lysosomes are acidic organelles that play multiple roles in various cellular oxidative activities such as the oxidative burst during cytotoxic killing. It remains to be determined how lysosomal lumen oxidative activity and pH interact and are regulated. Here, I report the use of fluorescent probes to measure oxidative activity and pH of lysosomes in live macrophages upon treatment with the tumor promotor phorbol 12-myristate 13-acetate (PMA), and provide novel insight regarding the regulation of lysosomal oxidative activity and pH.
        
        
          Results
          The substrate used to measure oxidative activity was bovine serum albumin covalently coupled to dihydro-2', 4,5,6,7,7'-hexafluorofluorescein (OxyBURST Green H2HFF BSA). During pulse-chase procedures with live macrophages, this reduced dye was internalized via an endocytic pathway and accumulated in the lysosomes. Oxidation of this compound resulted in a dramatic increase of fluorescence intensity. By using low-light level fluorescence microscopy, I determined that phorbol ester treatment results in increased oxidative activity and pH elevation in different subsets of lysosomes. Furthermore, lysosomes with stronger oxidative activity tended to exclude the acidotropic lysosomal indicator, and thus exhibit higher alkalinity.
        
        
          Conclusions
          Results indicate that there is a regulatory mechanism between lysosomal oxidative activity and pH. Activation of lysosomal Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase by phorbol ester may result in increase of intralysosomal O2•- and H2O2, concurrent with pH elevation due to consumption of H+ and generation of OH-. Furthermore, the effect of phorbol ester on elevated oxidative activity and pH is heterogeneous among total lysosomal population. Higher oxidative activity and/or pH are only observed in subsets of lysosomes.

Tags: lysosomal-ph, macrophages, nih, oxidative-stress, phorbol-ester
Organization: U.S. Department of Health & Human Services
Antitumor effect of lysine-isopeptides

Isopeptides (ε-peptides) of lysine, with a given Mw and low polydispersity (10–400 units), were synthesized to study the relationship between their chemical structure and biological effect. The designed compounds were of high purity, low polydispersity and high stereochemical purity. The effect of the compounds was tested on a human erythroleukemia cell line (K-562) and on four transplantable mouse tumors (L1210 lymphoid leukemia, P38 macrophage derived tumor, Ehrlich ascites carcinoma, Lewis lung tumor /LLT/). In case of the L1210 and P388 tumors and the Ehrlich carcinoma, survival of the animals was used as an indicator of the effect. In case of the Lewis lung tumor, the number and size of metastases in the lung and/or liver of treated and untreated mice were used as indicators. The polymers of polymerisation degree 80–120 (Mw 10.2–15.4 KD) showed the strongest antiproliferative effect both on K562 cells and the tumors growing in vivo. This effect was manifest with a significantly higher survival rate as compared to the control (L1210, P38, Ehrlich ascites), furthermore, by a decrease in the number and size of liver and lung metastases (LLT).

Tags: cancer-metastasis, erythroleukemia, lysine-isopeptides, nih, tumor-suppression
Organization: U.S. Department of Health & Human Services
Uniform Facility Data Set (UFDS): 1999 Data on Substance Abuse Treatment Facilities

This report presents results from the 1999 Uniform Facility Data Set (UFDS), an annual survey of facilities providing substance abuse treatment. Conducted by the Substance Abuse and Mental Health Services Administration (SAMHSA), the UFDS is designed to collect data on the location, characteristics, and use of alcoholism and drug abuse treatment facilities and services throughout the 50 states, the District of Columbia, and other U.S. jurisdictions.

Tags: alcoholism-treatment, drug-abuse-treatment, samhsa, substance-abuse-treatment, treatment-facility-survey
Organization: U.S. Department of Health & Human Services
Table 8. Nonmedical Use of Pain Relievers in the Past Year among Persons Aged 12 or Older, by State and Substate Regions: Percentages, Annual Averages Based on 2008, 2009, and 2010 NSDUHs

This table presents the 2008 to 2010 National Survey on Drug Use and Health (NSDUH) estimates of past year nonmedical use of pain relievers by those aged 12 or older by State and substate regions.

Tags: adolescent-health, nsduh, pain-reliever-misuse, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Recent Declines in Adolescent Inhalant Use

This National Survey on Drug Use and Health (NSDUH) short report shows inhalant use by adolescents 12 to 17 years old. It also looks at inhalant use by gender, race, region of the country, metro area, past year use and days used.

Tags: adolescent-inhalant-use, nsduh-data, samhsa, substance-use-trends, youth-drug-use
Organization: U.S. Department of Health & Human Services
A criterion audit of women's awareness of blood transfusion in pregnancy

Background
          In the Confidential Enquiry into Maternal Deaths (CEMD) Report, the very high risk of mortality in women who refuse blood transfusions is highlighted. The objectives were to establish current knowledge about, and views of transfusion in our pregnant population and to establish the level of compliance with the set audit standard.
        
        
          Method
          Questionnaire survey of 228 women, including both high and low risk pregnancies, attending ante-natal clinic between 2–9 May 2000 at the North Staffordshire Maternity Hospital, Stoke on Trent.
        
        
          Results
          The response rate was 100%. Only 43% were aware of the possible need for blood transfusion in pregnancy. If a blood transfusion was required, 92% stated that they would accept a blood transfusion in pregnancy. Four percent stated that they would not accept a transfusion because of religious reasons and risk of infection and the remaining four percent did not declare a reason.
        
        
          Conclusions
          This short survey identified that 57% of women were not aware of the possible need for blood transfusion during pregnancy. There is a need for more information to be shared on this subject with all antenatal women. Women who would refuse a transfusion need to be identified at booking and be referred for counselling and a management plan made for pregnancy, labour and delivery.

Tags: blood-transfusion, maternal-health, nih, patient-awareness, pregnancy-complications
Organization: U.S. Department of Health & Human Services
2019 Uniform Reporting System (URS) Table for Florida

These are the 2019 Uniform Reporting System (URS) Output Tables for Florida. The URS state reporting system is collected annually to support the Community Mental Health Services Block Grant program. State Mental Health Authorities report on National Outcome Measures (NOMS), evidence-based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: florida-urs-data, mental-health-services, national-outcome-measures, samhsa, substance-abuse
Organization: U.S. Department of Health & Human Services
2014 CMHS Uniform Reporting System (URS) Table for American Samoa

2014 Center for Mental Health Services (CMHS) Uniform Reporting System (URS) Output Tables for American Samoa. The URS territories reporting system is collected annually to support the Community Mental Health Services Block Grant program. Territories Mental Health Authorities report on National Outcome Measures (NOMS), evidence based practices, and utilization measures providing an overview of state mental health delivery systems.

Tags: american-samoa, mental-health-services, noms-measures, samhsa, urs-data
Organization: U.S. Department of Health & Human Services
5.2b Clients in treatment, according to type of care received, by facility operation and primary focus of facility: March 31, 2011. Percent distribution (row percent)

This is a National Survey of Substance Abuse Treatment Services (N-SSATS) data table showing clients in treatment, according to type of care received, by facility operation and primary focus of facility in 2011. N-SSATS is designed to collect data on the location, characteristics, and use of alcohol and drug abuse treatment facilities and services throughout the 50 States, the District of Columbia, and other U.S. jurisdictions.

Tags: facility-characteristics, n-ssats, samhsa, substance-abuse-treatment, treatment-services
Organization: U.S. Department of Health & Human Services
4.2a Type of care offered, by facility operation and primary focus of facility: 2011. Number

This a National Survey of Substance Abuse Treatment Services (N-SSATS) data table showing the type of care offered, by facility operation and primary focus of facility in 2011.

Tags: behavioral-health-services, facility-characteristics, n-ssats, samhsa, substance-abuse-treatment
Organization: U.S. Department of Health & Human Services
Publications Using SAMHSA DataTable 7. Cocaine Use in the Past Year, by Age Group and State: Percentages, Annual Averages Based on 2013 and 2014 NSDUHs

Publications Using SAMHSA DataTable 7. Cocaine Use in the Past Year, by Age Group and State: Percentages, Annual Averages Based on 2013 and 2014 NSDUHs

Tags: cocaine-use, drug-prevalence, nsduh-data, samhsa, substance-use
Organization: U.S. Department of Health & Human Services
Availability of Supported Employment in Specialty Mental Health Treatment Facilities and Facility Characteristics: 2014

This short report describes the number, percent, and characteristics of specialty mental health treatment facilities in the United States that offered supported employment, an evidence-based practice for individuals with serious mental illness to gain employment.

Tags: mental-health-services, samhsa, serious-mental-illness, supported-employment, treatment-facilities
Organization: U.S. Department of Health & Human Services
Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene

Background:
          The serine hydroxymethyltransferase processed pseudogene SHMT-ps1 has been suggested to be unique to the order Primates because of the failure to amplify this sequence by PCR from genomic DNAs of any non-primate mammal species. Here, 'molecular beacon' probes specific to SHMT-ps1 were used in an attempt to verify this suggestion.
        
        
          Results:
          In a search for SHMT-ps1-specific sequences using molecular beacons across a range of mammalian species, SHMT-ps1 was only found in primates. The molecular beacon assays also showed that SHMT-ps1 is present in both Old World and New World species but not among prosimians.
        
        
          Conclusions:
          These results suggest that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago.

Tags: gene-evolution, molecular-beacon-assay, nih, primate-genetics, shmt-ps1-pseudogene
Organization: U.S. Department of Health & Human Services
2013 NSDUH Sampling Error Report

This report compares the estimated (or realized) precisions of a key set of estimates with the targets for the 2013 National Survey on Drug Use and Health (NSDUH), formerly called the National Household Survey on Drug Abuse (NHSDA). The comparison was made with targets specified by the Substance Abuse and Mental Health Services Administration (SAMHSA) and with the predicted precisions that statisticians from RTI International anticipated during the design of the survey. In addition, tables of realized DEFFs are provided. This report is organized as follows. Chapter 2 summarizes the 2013 sample design. Chapter 3 describes the calculation of relative standard errors (RSEs) and DEFFs. Chapter 4 presents tables that compare the observed precisions with the expected precisions. Chapter 5 compares median and mean DEFFs. Chapter 6 presents median and mean DEFFs for specific analysis domains. Finally, concluding remarks are provided in Chapter 7.

Tags: mental-health, samhsa, sampling-error, substance-use, survey-design
Organization: U.S. Department of Health & Human Services
Long-term cultivation of colorectal carcinoma cells with anti-cancer drugs induces drug resistance and telomere elongation: an

Background
          The role of telomerase activation in the expression and/or maintenance of drug resistance is not clearly understood. Therefore, we investigated the relationships, among the telomerase activity, telomere length and the expression of multidrug resistance genes in colorectal cancer cell lines cultivated with anti-cancer drugs.
        
        
          Methods
          LoVo and DLD-1 cells were continuously grown in the presence of both CDDP and 5-FU for up to 100 days. Cell proliferation, telomerase activity, telomere length and the expression of multidrug resistance genes were serially monitored as the PDL increased.
        
        
          Results
          The expression of multidrug resistance genes tended to increase as the PDL increased. However, an abnormal aneuploid clone was not detected as far as the cells were monitored by a DNA histogram analysis. Tumor cells showing resistance to anti-cancer drugs revealed a higher cell proliferation rate. The telomere length gradually increased with a progressive PDL. The telomerase activity reached a maximum level at 15 PDL in LoVo cells and at 27 PDL in DLD-1 cells. An increase in the mRNA expression of the telomerase components, especially in hTERT and in hTR, was observed at the same PDLs.
        
        
          Conclusions
          These results suggest that a high telomerase activity and an elongation of telomeres both appear to help maintain and/or increase drug resistance in colorectal cancer cells. Cancer cells with long telomeres and a high proliferative activity may thus be able to better survive exposure to anti-cancer drugs. This is presumably due to an increased chromosome stability and a strong expression of both mdr-1 and MRP genes.

Tags: colorectal-cancer, drug-resistance, nih, telomerase-activity, telomere-length
Organization: U.S. Department of Health & Human Services
2016-2017 NSDUH National Maps of Prevalence Estimates by State

The 116 national maps presented here show the 2016-2017 National Survey on Drug Use and Health (NSDUH) estimates for 30 substance use and mental health outcomes, by age group, for 50 statesand the District of Columbia.

Tags: mental-health, nsduh-data, samhsa, state-prevalence, substance-use
Organization: U.S. Department of Health & Human Services
Proteinase-3 as the major autoantigen of c-ANCA is strongly expressed in lung tissue of patients with Wegener's granulomatosis

Proteinase-3 (PR-3) is a neutral serine proteinase present in azurophil granules of human polymorphonuclear leukocytes and serves as the major target antigen of antineutrophil cytoplasmic antibodies with a cytoplasmic staining pattern (c-ANCA) in Wegener's granulomatosis (WG). The WG disease appears as severe vasculitis in different organs (e.g. kidney, nose and lung). Little is known about the expression and distribution of PR-3 in the lung. We found that PR-3 is expressed in normal lung tissue and is upregulated in lung tissue of patients with WG. Interestingly, the parenchymal cells (pneumocytes type I and II) and macrophages, and not the neutrophils, express PR-3 most strongly and may contribute to lung damage in patients with WG via direct interaction with antineutrophil cytoplasmic antobodies (ANCA). These findings suggest that the PR-3 expression in parenchymal cells of lung tissue could be at least one missing link in the etiopathogenesis of pulmonary pathology in ANCA-associated disease.

Tags: autoantigen-expression, lung-vasculitis, nih, proteinase-3, wegeners-granulomatosis
Organization: U.S. Department of Health & Human Services
Effect of a short-term in vitro exposure to the marine toxin domoic acid on viability, tumor necrosis factor-alpha, matrix metalloproteinase-9 and superoxide anion release by rat neonatal microglia

Background
          The excitatory amino acid domoic acid, a glutamate and kainic acid analog, is the causative agent of amnesic shellfish poisoning in humans. No studies to our knowledge have investigated the potential contribution to short-term neurotoxicity of the brain microglia, a cell type that constitutes circa 10% of the total glial population in the brain. We tested the hypothesis that a short-term in vitro exposure to domoic acid, might lead to the activation of rat neonatal microglia and the concomitant release of the putative neurotoxic mediators tumor necrosis factor-α (TNF-α), matrix metalloproteinases-2 and-9 (MMP-2 and -9) and superoxide anion (O2-).
        
        
          Results
          In vitro, domoic acid [10 μM-1 mM] was significantly neurotoxic to primary cerebellar granule neurons. Although neonatal rat microglia expressed ionotropic glutamate GluR4 receptors, exposure during 6 hours to domoic acid [10 μM-1 mM] had no significant effect on viability. By four hours, LPS (10 ng/mL) stimulated an increase in TNF-α mRNA and a 2,233 % increase in TNF-α protein In contrast, domoic acid (1 mM) induced a slight rise in TNF-α expression and a 53 % increase (p < 0.01) of immunoreactive TNF-α protein. Furthermore, though less potent than LPS, a 4-hour treatment with domoic acid (1 mM) yielded a 757% (p < 0.01) increase in MMP-9 release, but had no effect on MMP-2. Finally, while PMA (phorbol 12-myristate 13-acetate) stimulated O2- generation was elevated in 6 hour LPS-primed microglia, a similar pretreatment with domoic acid (1 mM) did not prime O2- release.
        
        
          Conclusions
          To our knowledge this is the first experimental evidence that domoic acid, at in vitro concentrations that are toxic to neuronal cells, can trigger a release of statistically significant amounts of TNF-α and MMP-9 by brain microglia. These observations are of considerable pathophysiological significance because domoic acid activates rat microglia several days after in vivo administration.

Tags: domoic-acid, microglia-activation, neurotoxicity, nih, tumor-necrosis-factor
Organization: U.S. Department of Health & Human Services
Antinociceptive and antiedematogenic properties and acute toxicity of

Background
          Tabebuia avellanedae is a tree from the Bignoniaceae family. Commonly know as "pau d'arco" in Brazil, its inner bark is used as analgesic, anti-inflammatory, antineoplasic and diuretic at the Brazilian northeast. A validation of the plant usage has not been previously performed.
        
        
          Results
          Antinociceptive and antiedematogenic effects of Tabebuia avellanedae Lor. ex Griseb. inner bark were measured by nociceptive experimental models in mice. A rat paw edema test induced by carrageenan (1%) was also performed in rats to access the plant's antiedematogenic effect. The inner bark aqueous extract, administered via oral in three different concentration, namely 100, 200 and 400 mg/Kg, reduced the nociception produced by acetic acid (0.6% in water, i.p.) by 49.9%, 63.7% and 43.8%, respectively. The aqueous extract (200 and 400 mg/Kg, p.o.) reduced formalin (1%) effects only at the second phase of the experiment by 49.3% and 53.7%, respectively. Naloxone (5 mg/Kg, i.p.) was not able to revert the extract effect, however caffeine (10 mg/Kg, i.p.) reverted its effect by 19.8% at the second phase of the formalin test. The aqueous extract (200 mg/Kg, p.o.) inhibited edema by 12.9% when we used the rat paw edema model. The acute toxicity was low in mice.
        
        
          Conclusion
          The T. avellanedae inner bark aqueous extract presented antinociceptive and antiedematogenic activities at the used models, with a possible antinociceptive effect associated to the adenosine system.

Tags: anti-inflammatory, antinociception, nih, paw-edema, tabebuia-avellanedae
Organization: U.S. Department of Health & Human Services
Northeast (NE)

This report presents the Northeast\'s (e.g., combines Connecticut, Maine, Massachusetts, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, and Vermont) estimates for 25 measures of substance use and mental health based on the combined 2009 and 2010 National Surveys on Drug Use and Health (NSDUHs). Topics covered include past month substance use (e.g., illicit drugs, marijuana, cocaine, pain relievers, alcohol, tobacco), substance dependence, abuse, and treatment need, past year serious mental illness (SMI), any mental illness (AMI), suicidal thoughts, and major depressive episode (MDE). Resuts are provided for the Northeast for five age groups: 12 to 17, 18 to 25, 26 or older, 18 or older and all persons 12 or older.

Tags: mental-health, nsduh-data, regional-estimates, samhsa, substance-use
Organization: U.S. Department of Health & Human Services