Data from: Attraction, mobility, and preference by Lasioderma serricorne (F.) (Coleoptera: Ptinidae) to microbially-mediated volatile emissions by two species of fungi in stored grain

<p>Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (<em>Aspergillus flavus</em> or <em>Fusarium</em> spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by <em>L. serricorne</em>. Headspace collection revealed that the <em>Fusarium</em>- and <em>A. flavus</em>-inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with <em>A. flavus</em> compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to <em>L. serricorne</em> at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of <em>L.serricorne</em> during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.</p>
<div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: 4-way olfactometer assay.</p> <p>File Name: Sierra_2021_olfactometer_Ag_Data_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Release-recapture Assay .</p> <p>File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Ethovision Movement Assay .</p> <p>File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Headspace volatile collection assay.</p> <p>File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel" target="_blank">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Sequencing Data.</p> <p>File Name: sequencing_data.zip</p></li><br><li><p>Resource Title: File list.</p> <p>File Name: File_list_L_serricone_attraction_data.txt</p></li></ul>

Tags: ars, behavioral-ecology, coleoptera, data-gov, entomology, insects, ipm, microbes, mobility, mvocs, np304, olfaction, stored-products, trapping, volatile-responses
Organization: Department of Agriculture